LOCUS       DAFKMX010000001        70234 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFKMX010000001 DAFKMX010000000
VERSION     DAFKMX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 70234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.001"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1406
                     /locus_tag="LZB08_000001"
     CDS             <1..1406
                     /locus_tag="LZB08_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7313525.1"
                     /translation="IINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKMDT
                     TEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSKII
                     TNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVLLT
                     YPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQSTH
                     KTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGKEL
                     MEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQVEL
                     SNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPREA
                     FYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIVSG
                     LKDVDLNFIQIIDLEDI"
     gene            1537..2226
                     /locus_tag="LZB08_000002"
     CDS             1537..2226
                     /locus_tag="LZB08_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBM7313526.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2229..3164
                     /locus_tag="LZB08_000003"
     CDS             2229..3164
                     /locus_tag="LZB08_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBM7313527.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3161..4054
                     /locus_tag="LZB08_000004"
     CDS             3161..4054
                     /locus_tag="LZB08_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBM7313528.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4132..4878
                     /locus_tag="LZB08_000005"
     CDS             4132..4878
                     /locus_tag="LZB08_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBM7313529.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            4879..5712
                     /gene="rsmI"
                     /locus_tag="LZB08_000006"
     CDS             4879..5712
                     /gene="rsmI"
                     /locus_tag="LZB08_000006"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBM7313530.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            5735..6439
                     /locus_tag="LZB08_000007"
     CDS             5735..6439
                     /locus_tag="LZB08_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBM7313531.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(6627..7055)
                     /locus_tag="LZB08_000008"
     CDS             complement(6627..7055)
                     /locus_tag="LZB08_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBM7313532.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7092..8522)
                     /locus_tag="LZB08_000009"
     CDS             complement(7092..8522)
                     /locus_tag="LZB08_000009"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBM7313533.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            8654..9238
                     /locus_tag="LZB08_000010"
     CDS             8654..9238
                     /locus_tag="LZB08_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBM7313534.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9254..9775
                     /locus_tag="LZB08_000011"
     CDS             9254..9775
                     /locus_tag="LZB08_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBM7313535.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    9841..10087
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10171..12108
                     /gene="metG"
                     /locus_tag="LZB08_000012"
     CDS             10171..12108
                     /gene="metG"
                     /locus_tag="LZB08_000012"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBM7313536.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12138..12908
                     /locus_tag="LZB08_000013"
     CDS             12138..12908
                     /locus_tag="LZB08_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBM7313537.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13246..13677
                     /gene="phnG"
                     /locus_tag="LZB08_000014"
     CDS             13246..13677
                     /gene="phnG"
                     /locus_tag="LZB08_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBM7313538.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            13692..14267
                     /gene="phnH"
                     /locus_tag="LZB08_000015"
     CDS             13692..14267
                     /gene="phnH"
                     /locus_tag="LZB08_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBM7313539.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14270..15355
                     /locus_tag="LZB08_000016"
     CDS             14270..15355
                     /locus_tag="LZB08_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBM7313540.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15375..16244
                     /locus_tag="LZB08_000017"
     CDS             15375..16244
                     /locus_tag="LZB08_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBM7313541.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     DPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16237..17103
                     /locus_tag="LZB08_000018"
     CDS             16237..17103
                     /locus_tag="LZB08_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7313542.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17144..17857
                     /locus_tag="LZB08_000019"
     CDS             17144..17857
                     /locus_tag="LZB08_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7313543.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            17858..19027
                     /locus_tag="LZB08_000020"
     CDS             17858..19027
                     /locus_tag="LZB08_000020"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBM7313544.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19031..19867
                     /locus_tag="LZB08_000021"
     CDS             19031..19867
                     /locus_tag="LZB08_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBM7313545.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            19868..20863
                     /locus_tag="LZB08_000022"
     CDS             19868..20863
                     /locus_tag="LZB08_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7313546.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            20881..22569
                     /locus_tag="LZB08_000023"
     CDS             20881..22569
                     /locus_tag="LZB08_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBM7313547.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            22581..23648
                     /locus_tag="LZB08_000024"
     CDS             22581..23648
                     /locus_tag="LZB08_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7313548.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24085..25080
                     /locus_tag="LZB08_000025"
     CDS             24085..25080
                     /locus_tag="LZB08_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7313549.1"
                     /translation="MSFLQAKNVGKSYGQVKVLKDISIDIEKGEFICLLSPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25098..26786
                     /locus_tag="LZB08_000026"
     CDS             25098..26786
                     /locus_tag="LZB08_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBM7313550.1"
                     /translation="MTTIKNKRRISDSKIQKCFLVFLVIALLVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            26798..27865
                     /locus_tag="LZB08_000027"
     CDS             26798..27865
                     /locus_tag="LZB08_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7313551.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28057..28587
                     /gene="rnmV"
                     /locus_tag="LZB08_000028"
     CDS             28057..28587
                     /gene="rnmV"
                     /locus_tag="LZB08_000028"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBM7313552.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            28758..29627
                     /gene="rsmA"
                     /locus_tag="LZB08_000029"
     CDS             28758..29627
                     /gene="rsmA"
                     /locus_tag="LZB08_000029"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBM7313553.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEIGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            29693..30955
                     /locus_tag="LZB08_000030"
     CDS             29693..30955
                     /locus_tag="LZB08_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313554.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDSEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31106..32221
                     /locus_tag="LZB08_000031"
     CDS             31106..32221
                     /locus_tag="LZB08_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7313555.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYEKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            32407..33303
                     /locus_tag="LZB08_000032"
     CDS             32407..33303
                     /locus_tag="LZB08_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBM7313556.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            33373..34170
                     /locus_tag="LZB08_000033"
     CDS             33373..34170
                     /locus_tag="LZB08_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313557.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34225..35577)
                     /locus_tag="LZB08_000034"
     CDS             complement(34225..35577)
                     /locus_tag="LZB08_000034"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBM7313558.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            35796..36674
                     /gene="purR"
                     /locus_tag="LZB08_000035"
     CDS             35796..36674
                     /gene="purR"
                     /locus_tag="LZB08_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBM7313559.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            36830..37108
                     /gene="spoVG"
                     /locus_tag="LZB08_000036"
     CDS             36830..37108
                     /gene="spoVG"
                     /locus_tag="LZB08_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBM7313560.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37321..38700
                     /gene="glmU"
                     /locus_tag="LZB08_000037"
     CDS             37321..38700
                     /gene="glmU"
                     /locus_tag="LZB08_000037"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBM7313561.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITYDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            38795..39745
                     /locus_tag="LZB08_000038"
     CDS             38795..39745
                     /locus_tag="LZB08_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBM7313562.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      40584..40669
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            40831..41346
                     /locus_tag="LZB08_000039"
     CDS             40831..41346
                     /locus_tag="LZB08_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBM7313563.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGEYKLIKVG
                     SKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTNAALSAKLTSTTKVNNEALYIVL
                     IDNIVMDQQGA"
     gene            41475..43151
                     /locus_tag="LZB08_000040"
     CDS             41475..43151
                     /locus_tag="LZB08_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBM7313564.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43163..44371
                     /locus_tag="LZB08_000041"
     CDS             43163..44371
                     /locus_tag="LZB08_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBM7313565.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            44386..46098
                     /locus_tag="LZB08_000042"
     CDS             44386..46098
                     /locus_tag="LZB08_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBM7313566.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDIQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46091..46999
                     /gene="pilO"
                     /locus_tag="LZB08_000043"
     CDS             46091..46999
                     /gene="pilO"
                     /locus_tag="LZB08_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBM7313567.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47026..47592
                     /locus_tag="LZB08_000044"
     CDS             47026..47592
                     /locus_tag="LZB08_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBM7313568.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            47629..48156
                     /locus_tag="LZB08_000045"
     CDS             47629..48156
                     /locus_tag="LZB08_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBM7313569.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48177..49715
                     /locus_tag="LZB08_000046"
     CDS             48177..49715
                     /locus_tag="LZB08_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313570.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPDQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            49815..50897
                     /locus_tag="LZB08_000047"
     CDS             49815..50897
                     /locus_tag="LZB08_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBM7313571.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            50878..51660
                     /locus_tag="LZB08_000048"
     CDS             50878..51660
                     /locus_tag="LZB08_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBM7313572.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKRKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            51762..52412
                     /locus_tag="LZB08_000049"
     CDS             51762..52412
                     /locus_tag="LZB08_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBM7313573.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            52426..52986
                     /locus_tag="LZB08_000050"
     CDS             52426..52986
                     /locus_tag="LZB08_000050"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBM7313574.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53003..56389
                     /gene="mfd"
                     /locus_tag="LZB08_000051"
     CDS             53003..56389
                     /gene="mfd"
                     /locus_tag="LZB08_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBM7313575.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            56420..57415
                     /locus_tag="LZB08_000052"
     CDS             56420..57415
                     /locus_tag="LZB08_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBM7313576.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            57539..58078
                     /gene="spoVT"
                     /locus_tag="LZB08_000053"
     CDS             57539..58078
                     /gene="spoVT"
                     /locus_tag="LZB08_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBM7313577.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58198..59811
                     /locus_tag="LZB08_000054"
     CDS             58198..59811
                     /locus_tag="LZB08_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBM7313578.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            59918..61366
                     /gene="mazG"
                     /locus_tag="LZB08_000055"
     CDS             59918..61366
                     /gene="mazG"
                     /locus_tag="LZB08_000055"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBM7313579.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            61455..61733
                     /locus_tag="LZB08_000056"
     CDS             61455..61733
                     /locus_tag="LZB08_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBM7313580.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            61805..62047
                     /locus_tag="LZB08_000057"
     CDS             61805..62047
                     /locus_tag="LZB08_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBM7313581.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            62109..62363
                     /locus_tag="LZB08_000058"
     CDS             62109..62363
                     /locus_tag="LZB08_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBM7313582.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            62364..62897
                     /locus_tag="LZB08_000059"
     CDS             62364..62897
                     /locus_tag="LZB08_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313583.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            62903..63187
                     /locus_tag="LZB08_000060"
     CDS             62903..63187
                     /locus_tag="LZB08_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBM7313584.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63300..64220
                     /locus_tag="LZB08_000061"
     CDS             63300..64220
                     /locus_tag="LZB08_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBM7313585.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64310..66682
                     /gene="spoIIE"
                     /locus_tag="LZB08_000062"
     CDS             64310..66682
                     /gene="spoIIE"
                     /locus_tag="LZB08_000062"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBM7313586.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICVSKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67033..68925
                     /gene="pepF"
                     /locus_tag="LZB08_000063"
     CDS             67033..68925
                     /gene="pepF"
                     /locus_tag="LZB08_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBM7313587.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69039..70004
                     /gene="galU"
                     /locus_tag="LZB08_000064"
     CDS             69039..70004
                     /gene="galU"
                     /locus_tag="LZB08_000064"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBM7313588.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        28302 a         7568 c        12676 g        21688 t
ORIGIN      
        1 ttattattaa tgaattaaat aagattgtaa atgaagaagt aatatctttt catatgcctg
       61 gtcataaaaa gggacaggtt tataaaaagt taggatataa agatatacta aaaaatttgt
      121 ataaaatgga tacaactgag atattaggaa cagataattt acactttccg gaaggaatta
      181 taaaaaattc acaaaaaaaa gcatcaagag tatttaaaag tgattatact tgctatttag
      241 taaatggtag tagctgtgga atacaatctg caataatgtc agtatgtagt cctaaaagta
      301 aaataataac aaatagagac tgtcatcagt ctgttataaa tggatgtatt ttaggagata
      361 tagatataga gtatgtgcct tgtgagataa gtgaagagac aaatgtctta aaagggatat
      421 ctataagtaa tgtgattgat ataatagata aaaacttgga tgcaaaagca gtactgctta
      481 cttatcctac atattatgga atgacatatg atttagaata catatgtaat tatgcacata
      541 gcaaaaagat ggtagtgata gtagatgaag cacatggtgc gcatttaggg cttagcgaga
      601 ggcttcctaa aacagctctt gagcaaggcg cagatatagt tgtacagagt acacataaaa
      661 ctcttccagc atttacacaa tcgtcaatga tacatataca gggagaccga gttgatatag
      721 ataaaatatt gaacatgtta agaattacag aatcttcaag cccttcatat ttattattat
      781 cttctctgga attagcagta gatatatatg aaaataaagg aaaagaatta atggaagagc
      841 ttttaaataa tattataact ttcaaaaata aagttgattc aaatattaat atatacgaca
      901 caaatgataa aacaaaaata tttatatcat ctaaaaatat gggtttaaca ggttatgaac
      961 tagaaaatat tttaagaaac aaatataaca tacaagtgga gttatctaat tattatggag
     1021 ttcttttaat atgtacaata ggaaatactg ttgaggattt ctctagttta gaaacagcat
     1081 taaacaatat tgtaaaggaa gactttaaat ttgaaaagtt agaaaataaa aaataccctg
     1141 ttgttattcc aaagaaaata ttaacaccta gagaagcatt ttataaaact aaaaaaagtg
     1201 ttaaaatata tgacagcata ggtaaagttt gtggtgaatg tatagttcca tacccacctg
     1261 gaataagtgt aatatctcct ggtgaagtaa taagtaaaga aataatagac tatctaatgt
     1321 tttgtcattc taaaggcatg attgtgagtg gattgaaaga tgtagattta aattttatac
     1381 aaataataga tttagaagac atttaaaaat tatgatataa aagtagatag aagtagatag
     1441 tttaaaattg tgaagagaaa taaatagact gcttatatag agctatctaa taaaagtact
     1501 aaaaaagtaa aaagtgtttg tatggaggat atgaaaatga aaggtaagtt aataattata
     1561 gagagtggtt cagatgcaag tggaaaggct actcagacag aaatattata tgaaagatta
     1621 aaaaaagaag gctatatgat taaaaaggta gaatatccaa attatgaatc tccatcatct
     1681 gccttggtga aaatgtacct aaaaggagac tttggtaaaa atgcatcaga tgtagaccca
     1741 tatatagcat caactttttt tacagctgat aggtatgcat catttaaaac cgattgggag
     1801 gagttttaca atgaaggtgg aataattatc gcagatagat atacaacctc taatatggta
     1861 catcaggcct ctaaaatgga tatttgtgat agagaagagt atttaaattg gttagctgat
     1921 tatgaattta atttatttaa gataccacaa cctgattgtg ttatattttt agatgtgcct
     1981 attgaattta gtaaaaagct tatggagaat agaaaaaata aaattacagg ggaagaaaaa
     2041 aaggatattc atgaaagtga catagagtat ttagagaaat cttacaacaa tgctttatat
     2101 atagcagata aatatgattg gaaaaaaata aattgtgttt tagataacaa attaagaagt
     2161 attgaaagta tacataatga aatttataaa atagttcttg attcaataaa acctatggag
     2221 agataaatat gtattttgaa aatattatag ggcaagattt tgcaaagaaa tatttaacaa
     2281 attctataaa gaaaaataaa cttaataatg cgtatatgtt tgaaggaatg gatggaatag
     2341 gaaaaaagaa atttgcagat gaattatcaa aactattact tgattatgaa aatctcgaaa
     2401 atagtccaga ctatgtactc ataaaaccag atggaaatag tataaaaata gctcaaatca
     2461 gaaatttaca atcagatata gtaataagac ctcataaaga ctataaaata tatataatca
     2521 ataatgctga aaaaatgact gtggaagctc aaaatgcact tttaaagacg ctagaggaac
     2581 ctccaaacta tgctataata atcttagtca caaacaacaa ggaatcttta ttagaaacta
     2641 taaaatctag atgtgatata ataaagttct cacctatacc aattgaagac ttaaaacgtt
     2701 atttgataaa tacaggtata gaagaagaaa gagctcaatt attggctatt ttttcgagag
     2761 gaagtattga gaatgctcta aatctatctc agtcttctga gttttcaatg atgagagaag
     2821 atatacaaca gtatattcag ataatgttag ataaaaacat agtggagatt ttaaatatac
     2881 caaataatat ggaaaaatat agaggtaaga ttatagcttt attagatatg atgataaact
     2941 attttcgaga tatcatccta ttaaaagaaa atgtaaacaa gaacatgtta ataaatgtag
     3001 ataagctagt tttcattcaa aatatgagtg gaaaaattag ttattctcaa ttgtccaaga
     3061 ttattgatat aatagaagat gcaaagagta aaataaaaag caattgcaat ttcaatataa
     3121 gcatacaagt tatgtcttta aatatatacg aggtgattaa atgataaaga tagtaggtat
     3181 aaggtttaaa agtgctggga aaatatatta ttttgaccct gttgatttta atatagagca
     3241 agatatggat gtagttgtag aaactgcaag aggcttggag tatgggaaag tagtcgttgg
     3301 tccaaaagat atggatgaaa gcgaattagt ttctcctttg aagccaatta taagaatagc
     3361 tacagaagaa gataaaaaag tatatctaga aaataaggaa aaggcaaaag aaacttttga
     3421 gttgtgccaa caaaaaataa aagaacatga gttaacaatg tttttaatag attgtgagta
     3481 tacatttgat aggaataagt tgatttttta ctttacagca gaaggaagaa tagattttag
     3541 agaattggta aaagacttag ctgctatatt taaaactaga atagaactta gacaaatagg
     3601 tgtcagagat gaagctaaat ctataggagg tctagggcct tgtggtagaa aactttgttg
     3661 ttcatcttgg ttaggtgatt ttcaaccagt ttcaataaaa atggcaaaag accaaagtct
     3721 atctctaaat ccaacaaaaa tatctggaat atgtgggaga ttattctgtt gcttaaaata
     3781 tgagcatgat gtatatagtg aagcaataga aaaaatgcct gttgtaggtg cattggtgca
     3841 atcagatgat ggaaaaggaa aagttgtaga agtgaatcct ttattagaac aaattaaagt
     3901 ggaatttcaa gacaagacag taaagatata cacaagagaa gatataaaaa tacttagaga
     3961 gcctaaaaaa tgtgatggct gtggtggtaa atgtaaggat aatgatggat tagatgaagc
     4021 cacactaaga gagctaaaga aattagaaga ttaaatttaa ggtagggctt ttcctacctt
     4081 ttttaaagca attaatgctt aaagttatta atatttgagg agaatttata tatgcaatta
     4141 aaagattcag aaagaataga tgatttacaa ttaaaaggcc ttaaaattat acaagatact
     4201 aatggttttt gttttggtat agatgcagtt ttattagcca attttactaa aataaaaaaa
     4261 gatgccaaag tagtagattt gggtactgga actggaatta tacctatttt gatagctgga
     4321 aaaagtgaag ctaaaaaaat tattggcgtg gaaatacaag aagatgtata tgaaatggca
     4381 actcgttcta taaaattaaa tggattagaa gaaagagttg agattataaa tgaagatata
     4441 aaatacatag acaaagtctt agacataaat ggttaccatg tagtcacatc aaatcctcca
     4501 tatatgcata ttgatggaat aaaaaatcca aatgataaaa aagcaatatc aagacatgaa
     4561 gtaaggtgca atttggaaga tgtaattaga gctgcatcca gattggttat gcctagagga
     4621 aaattcttca tgatacatag accaacaaga ttggttgata taataacttt agggagaaaa
     4681 tataaattag aaccaaaggt tattcaattt gtgcatccaa gacctcaaaa agcaccgaac
     4741 ttagttttgg ttcaatttgt aaaagatgga aggccagagc ttaaaatatt ggacccattg
     4801 tatgtttatg gagaagatgg gaattataca aaagaattaa aagcaatata taataataaa
     4861 gatataggag agttgtaaat gagcgggaaa ttgtacatat gtccaacacc aataggaaac
     4921 ctagaagata taacatatag aactttaaga atattaaaag aagtagactt gattgcagca
     4981 gaagatacaa gacatagtat aaaactttta aatcattttg aaatctcaaa accactaact
     5041 agttatcatg aacataataa ggattcaaag ggagattatt tgataaataa actaattgat
     5101 ggtgaaaata tagctctgat aagtgatgca ggtatgcctg gtatatctga cccaggagaa
     5161 gagataataa aacaagctat acaaaataac atagagatag aggtattgcc aggtgcaaca
     5221 gcctttgtaa cggctttagt tggctctggt atggacacac atagatttgt ttttgagggc
     5281 tttttagata gagataaaaa agttaggaaa gcacaattgg aagaattaaa ggaagaaagt
     5341 agaactataa ttttctatga atcaccacat agattgaagg atacactaaa agatatgctt
     5401 aaaattcttg gtaataggaa tatttctata aatagagaac ttacaaaaaa atatcaagaa
     5461 ataataagag aaaatataga aactaccata aaaatatttg aggaaaaaga agtaagagga
     5521 gagtttgtat taatagtaga gggttttcat ggtgagaagt caaagaaaga aaattatgat
     5581 gggcttacag atagggaata tgttttaaaa ctaattgaaa gtggtatgaa caaaaaagat
     5641 gcaataaaaa tagtttgtaa agatagaaag ttaaagaaag atgtggttta taaacaagtt
     5701 ttagatttat aaaacctgaa tctgaggtga ttttatggaa atagggaaaa ttataataga
     5761 gagtttaaag agggaagata cacataaaat atttatagaa ttagataata agcatgaatt
     5821 agaaaaaata attcctaaaa taagctgtat gaagtctgta ggtgaatgta aatatcatgt
     5881 agtaaattgt tttgaacatt ctataaatgc attaaaagaa ttagaaatag tattaaatga
     5941 taaggatttt tttccaatac atctaagaaa acatgtgtca aactatttaa aaacatatat
     6001 tgaagaagga ataaataagt tacatgtatt aaagctggga acatttttac atgatatagg
     6061 taagcctgac agtatgacct tagatgaaac tggaagagtt catttcacaa atcatgaaaa
     6121 aataggtgct caaataattg acaatatggg aataaagtta gagttatcaa cagagacata
     6181 tagacttata agtaaatatg taagatatca tatgatactt ttgtctctat ataagaaaaa
     6241 tgatttgagt aggaaagaat taattaatgt atttaactta gtagatgagg acactatagg
     6301 tgtaataatg cttgggtatg cagatatagt gtctacagta aaattattgg gcaagagtgg
     6361 agaagtcagt gtgttaaaga catatatgga atatatttta acaaattact tgtataagag
     6421 taattataca catgtataaa tttagttaat ataattgagt tttagaggat taattgaagt
     6481 ttgaaatata cggatttcaa aaacataaaa gaagttgtct ttgttgtaaa attataaaag
     6541 acaacttttt ttatatcaaa aattgaaatt ttattaagga tacttaatgg tctattaatt
     6601 aataaaccat tagtatttaa tttatattac aatattgatt ttgcttgtat tggcgttgat
     6661 agtataacag aggtcttagt agaaccaatt ttctttatag tatcaatcac attttctagc
     6721 tcgtacatat cttttacaat tacttttaaa agtaagcaat catcacctgt tatatggtga
     6781 cattctacaa tcctagggtc ctttgcagct gactcaataa attctgtata tccattgctt
     6841 ggaagagaaa tatgaataaa tgcctttata actctgccta atgaatctgg gttgacaata
     6901 gctttatatc cttctataac accagactct tctaatcttt tgactctttc tgaaactgca
     6961 ggagaagtta aaccaactat ttttcctaag tccttcatag aaattctccc atcatcctgt
     7021 aaaatttcta tgattctgta atctgtaaca tccataaata aacagtcccc cttaaataaa
     7081 aataattaat gctataaaac ttttttagaa tctatcattt gtgtttttaa ataccaaaga
     7141 tttctagata aatcaacctt ataaatgtgt ggttttgaaa gtcttttgta ttgttcccag
     7201 atagtactta attcatattg tacatgattt tttatttcaa gaacagcttt tttattatat
     7261 ttgcatctgc cttttatata caatggttta agaagctctt taactttgta atttgtaaat
     7321 accttcgttt tccatgtata agtaggatgg aagatttcta aaggtttaga ctcatctata
     7381 acttcatcat gtaacataat caaatctgcc tctgccttat tgtcttcgtt atatattctt
     7441 ataactttct tgtaaccagg attatttatt ttttctggat tctcagaaat ctttatctta
     7501 ggttcaagaa ctcctttttc acaagatgca gctaatttat aaactcctcc aagagagggt
     7561 gaatcgaaag atgtaatgag ctttgtacct actccccatg aatttatagt agctccttct
     7621 gctttaagtg atgttatggt atattcgtct aggtcattag atgcagttat gctgatatct
     7681 gaaaagccag cattatctaa ttgtttttta gcttctttag ataaataagc taaatctcca
     7741 gagtcaagtc ttatacccat aggtttgtat cctttttcag aaatattttt gaatactttt
     7801 attgcattag gaactccact gtttaataca tcataagtat ctacaagtag taggcattta
     7861 tctggataaa catcagcata tgcttggaat gcttcaagtt cattatcaaa tttttgtacc
     7921 caactgtgag cttgtgtacc aattataggt atatcaaaca ttttccctgc tagtacgttt
     7981 gcagttccag cacaaccacc aactacggca gccctagccc catatatccc tgcatctgga
     8041 ccttgtgcac gacgtaatcc aaattcaaat acagggtctc cttgagctgc aaagcaaact
     8101 ctagatgctt tagtagcaat aagagactga aaattaacta ttgttaacaa tgctgtttca
     8161 ataagttgag cttgatataa aggtgcctta acagtaatta atggctcatt agggaacatg
     8221 atagttcctt cctcaacagc atatatatct cctgtgaatt taaattcttt taaaaatttt
     8281 aaaaacttgt cagaaaataa atttaagttt tttaagtact ctaaatcttc atctgaaaaa
     8341 tgaagattat ctatgtattc aacaacctga tcaataccac aaactatagt ataccctcca
     8401 tcacaagcgt tttttctaaa aaacatatca aatacaacaa tgtcttcatg aatattcttt
     8461 tcaaaatagc catttaacat agtaagctga tataaatcag taagaagtgt tagatttctc
     8521 attatttctc tccttaatta ttattaaata ttaaaaattg taaaaactcc cacacttata
     8581 ctaaaacata atatagtaca actttcaagt ataaagtatt acctaactat cataaaatta
     8641 tttaaagagg aggatgatta taataggaaa aatatggttt tatatggtat cgataggaat
     8701 aataggaagc ttgatttcta aaaatatggg tgaattaaat aaagttatat taagtgaaac
     8761 atcaaaaggt atagagtttg caataagtct agctggtatc atggcactct ggatggggat
     8821 aatgaatatc gcaaaagaat caggtttaat tgaaaagata ggtcaaaaac taaatcctat
     8881 aatgagaaga ttatttcctt ctattccaaa gggtcataaa gcaatgtctt atatagttat
     8941 gaatattgct ttaaatatgt tgggtgctgg aaatggtgca acagcatttg gtttaaaagc
     9001 aatgagtgag cttcaaactt tgaataacaa gaaagataca gccactaatg atatgattat
     9061 gtttatggta atcaatatat catctataca aataatccca tttaccataa tcaaattaag
     9121 gatggatatg ggggctcaaa atccaagtga aataattttt acgactttat ttgcaaccat
     9181 aatatctaca gcagtagcaa taataacatg taaatttttt caaagaagat atagataaag
     9241 gggatgtaga tacatggaga tattttcaaa cttacttata ccaataataa ttctgtacat
     9301 agtagtttat ggtaaatata aaaaaataga tgtgtatgat agttttgtaa aaggagcaat
     9361 tgatggatta aaagcagcat gggatatcct accatatata ataggtatat ttttggcaat
     9421 aggaatattc aaaactggta aaggtcttga tatgttagaa tggctattta caccaatagc
     9481 aaatatgatg agtataccaa aagaattaat aggtctaata agtgtaaaac ctttatctgg
     9541 aagtggtgct ttagggatgt acagtgaact tgcaaataga gttggaatag gaagtttagt
     9601 agaaaaaatg ggtgctacga ttgtaggttc ttctgagact attttttaca caatggcaat
     9661 atactatgga agtctgaaga taaagaatac tagacataca ttatcatgtg ctatgattag
     9721 ccatgttgca ggtgtcatag cagctgtttt tatttgctat gtaatatttg tataatttgt
     9781 tgacaatatg ggaaaaattt tataatatag tgattatata gaaacaaata gtaatacaaa
     9841 ttacaagcta tgaaaagaag agtaaataaa ggttttttct atcagagagc taggttaggt
     9901 gaaagctagt gtcaaaagct ttattgaaga tggtcttgga gctttttatc tgagcaagtg
     9961 tgctagggta aaacggttgg acacgttata gtctactaag gtatctataa aatgtttcta
    10021 catttcatgt gaaacataac tattatagat gaattagggt ggtaccgtga ataatctcgc
    10081 ccctatgtta actcatagga ggcgattttt tattatttaa atgaaagaat catatgatta
    10141 cgaacgataa aaaattagga ggtaaataac atgagcaaac cgagttttta tgtaacaaca
    10201 ccaatatact atccaagtgg aggattacac ataggacata cttattcaac agtagctgca
    10261 gatacaatag ctagattcaa gcgtttctgt ggatatgatg taaagttctt aacaggaaca
    10321 gatgagcatg gtgaaaaaat tcaaaagaaa gccatagaac aaggaatgtc agagatagag
    10381 tatcttgatg gaatgataaa agatataaag gccttatgga atactatgga tatatcttat
    10441 gatgatttta taagaactac agaaaaaaga catacagaca taatacaaaa gatatttact
    10501 aaactatatg agcaaggtga tatatataaa ggtgaatatg aaggtagata ctgtactcct
    10561 tgtgagagtt tctggacaga atctcaatta ctagaaggaa ataaatgtcc tgactgtgga
    10621 agagaaacat atttagtaaa agaagaatct tatttcttta gattatctaa atatgaagac
    10681 agattaaaag agttatttaa agatgatagt ttctgttttc ctgctgctag aaaaaatgaa
    10741 atggtggcaa actttttaga caaaggatta gaagatttaa gcgtaactag aacgactttt
    10801 gactggggta taaaagttcc ttttgatgaa aaacatgtaa tttatgtgtg ggtagatgct
    10861 ttatgtaact atataacagc attaggttat atgactgata atgatgaaga atttaaaaaa
    10921 tattggcctg ccaatgttca aatagtaggt aaagaaatag tgagattcca tacaataata
    10981 tggccagcgc ttttaatggc tttaggtcta gaagttccaa aacaagtatt tggacatgga
    11041 tggatactat ttgctgatga taaaatgagt aaatcaaaag gtaatgtggt ttatccagaa
    11101 cctataatag aaagatatgg aatagacaca cttaaatact tcttattaag agagtttgca
    11161 tttgggcaag atggaagtta tactcataga aattttgtaa cgagaataaa ttatgattta
    11221 gcaaatgatt taggaaactt aataagtaga actgtagcta tggttgaaaa atacaataat
    11281 ggcataatac caacagcaaa agtttcaact aattttgatg ctgacttaaa agaacaagct
    11341 gtttcaacta gagaaaactt tgaagctgaa atggataaaa tgcaattcca tgaagcatta
    11401 gagagtgtat ggaaattagt tagaagaact aacaaatata tagatgaaac tatgccttgg
    11461 gcactagcta aagatgaaac taagaaagat gaattagaca cagttttata taatttatgt
    11521 gaatctataa gaataatcgc aacactaata aatcctataa tgaatgagac tgctaataaa
    11581 atatatgaac atataggaat aaaaggtcaa gatgatataa ctacatggga aagtacaaaa
    11641 acatttggtc ttattggaga aaatgtaaaa gtatttaaag gtgaaccttt attcccaaga
    11701 ttagatgtag aaaaagagat agaagaatta actaaaatgt tctctgggaa acctccagta
    11761 gaagaaaaac ctttagagca taaagaagaa attactattg atgatttaga caaaatagag
    11821 cttagagtag ggaaaataat aagctgtgaa aaacatccaa aagcaaataa attattagta
    11881 tcacaagtta agattggacc agaaacaaga caaatagtat caggaatagc tgaatactat
    11941 aagccagagg atttagtagg aaaagaggtt acagtggtat gtaacttaaa accagttaaa
    12001 ttaagaggtg tagaatctca aggtatgata ttagcagcag gtgatgatgg agacccatat
    12061 gttttaccat ttacacaagg ggctaaagat ggatgcgagg ttcgttagta ggtaattaaa
    12121 tagtaaggag attaaagatg ttatttgact cacatgcaca tttaaatgat gaaagttttg
    12181 atgaagatag agatgaactt attggttcat taaaagataa aggtgtagat ttggttgtaa
    12241 atccaggagc agatatagag acgtcgataa cagcaattga acttgcgaaa aaatacgact
    12301 ttatatattc agcagttggg gtacatccac atgatgtatc aaaactagat gatacagcaa
    12361 tagaaactct aagaaaactt gcaactgaga atgaaaaagt tgtagctatt ggagagattg
    12421 gattagatta ttattatgat tattctccaa gagaagaaca aaaagagtgg tttaagaagc
    12481 aaattgaatt agctaatgag ctaaaacttc caataataat acatgataga gatgcacatg
    12541 gagatacttt tgagataata aagaatacta aaaatcctga aataggatgt gtacttcatt
    12601 gttatagtgg gaatgtagag ttagctagag aatatgttaa gatgggttgt tatatatcaa
    12661 ttccaggtac ggttactttt aagaataata aaaaaactag agaagtagta agagaaatac
    12721 ctctagaaag attattcata gagactgatt caccatatat gtcaccagag ccacatagag
    12781 gaaagagaaa caatccttcc caagtatctt ttgttgctga taagatagcc caggaaaaag
    12841 ggatatctta tgaagaagta tgtagagtta caaaagaaaa tgcaaagaaa ttttttaata
    12901 taaaatagac aaattaaatc ttataatgat atatataata agtcattata agtataggat
    12961 gagagtgcct caaaatgagt tttttagttt atgaggcact cttttttgta tgaaatcaaa
    13021 tatattttca tcatttcaac aatgaaaaag aagtttatca tattttgaga tagtttgttt
    13081 ttatttattt aattcatatt ttaaaatata ttttcttaac attgttaaaa aagaatttac
    13141 aagatattca ttagaattaa ctcaactcta atattcactt aacttaagtt gtttattata
    13201 ttattaaaga gaagtaaacg aaaggtagat taaggaggtt gaattttgta tacagttgat
    13261 agtactaaga tattgataaa tatgaactta gagaaactag aaaagatata ctcaatgata
    13321 aaagatacat caagtatttc tataattaaa gaacctaact tggctacagt aatggtaaga
    13381 gctaatgaaa gtgtaaaaaa cacaacattt aatttaggag agatattagt tactgaatgt
    13441 agtgtaaagg tagatgaaag cttgggttac ggaatagttt cagaaaacaa taataagaaa
    13501 gccatttact tagcagttat agatgcagtt ttacatagca ataacagtaa atttgatgag
    13561 ttaaagaatt acataaataa aagtgtatat gaagagaact taaggtatga acaagagatt
    13621 attgatgaat ttagcttgat taataaaacg aaagttcaat tcaatgctat ggattaaata
    13681 ggaggagaat catggtaaat tatacacaag aaatatatag aaagatgcta gatagtgctt
    13741 catttcctgg gaaattaaat aatatagata atataaatat agaaaataat acaaaactat
    13801 tgaatggagc aattggtata gcaatcactt tattagacca agaagttacc ttctatatag
    13861 aaaattatac tcaaaaagat ataaaaaata taagagcgct tacaatgtca aatcaaatag
    13921 attatagaaa atcagattat atatttttag atattaattc agaagtggat atattacaga
    13981 ttaaaatagg ttcttttgaa tatcctgatg aaagtgcaac tatagtacat caagtaaatg
    14041 atttatcaac gtactctcaa actaagtata taaagcttga tttgagtggt cctggtataa
    14101 aatctaaaaa tagtatatat ataaatggaa ttagagaaga atttatagaa agattatcta
    14161 ccataaataa agattatcca ataggaatag atttgatttt agtagataaa aaaggagaaa
    14221 ttgcatttat accaaggtca tcaaaattaa gttgggaggt attgtagaaa tggcttatgt
    14281 agcagttaaa ggtggagaag aagccatatt acaggctaaa aatatacttg agttttatag
    14341 ggtaaaagga gatagcttgc ctatagatgt aaagcagatt gaggaacaaa tgtcttatgc
    14401 tgtagacaaa gtaatgtctg aaggaagttt atacaacaag gaactatcag cattggctat
    14461 aaaacaagct caaggtgata tattagaagc ctctttctat gtaagagctt taaggtcaac
    14521 aatgcctaga attacatatt cagaacccat aaatacagaa gaaatgagaa taaaaagaag
    14581 aatatcagct acttttaaag atataccagg aggtcagtat cttggggcaa ctagagacta
    14641 tgaaaataga atattaaatg tagaattatt agaagaagat gaaaaaaaga atagacattt
    14701 tagagatagt tttataaatg aattagaaac agtagatgaa atccctgaat ttacaaaaat
    14761 atctaatata tttagaaaag aaaatttatt acaagaagaa gtttgtgaaa acaaattgga
    14821 agaagttatt gatgatatta caagaaatag tttaagattt ccagtttcta ggtcagcaaa
    14881 gttacaggca ttaactagag gtgaaagtgg agtagtgtca ggttttgctt atgctattgt
    14941 aagaggtttt ggtgatgaac atccatatat aagtgaactt agaactggta atgtatttat
    15001 aaaagtaaaa catcccatct atgaagatga agaaataata ataggggata tgctggttac
    15061 tgaatgtgaa atagtttcaa aagcatcaca aaataagact gaaaattcta aaactaattc
    15121 ggaagataaa aataagatgt ctttaggata tggtctttgt atggggaata atgagaataa
    15181 ggctatatct atggccatac tagacaaatc tttggagcca aatagtgaaa aactagcaca
    15241 agatgaagaa tttgtattgc ttcatataga tggaatagat tcattaggat ttatatctca
    15301 ctttaagctt cctcactatg tagattttaa agcggatgta gaaagaataa aatagtttgt
    15361 ggaggtaagt ttaggtgata tataactatg gttttttaga cgaaaacaca aaaaaagaaa
    15421 taagaagaaa gatattaaaa gcaatatcaa tacctggtta tcaagttccc tttggttcaa
    15481 gagagttgcc tatagctaag ggatggggta ctggaggttt acaattaaca ctatcattaa
    15541 ttggtaaaaa tgatgtagta aaagtaattg accaaggaag tgatgattct acaaatgctt
    15601 gtaatataag gagctttatt gcaagtgtaa gtaatgtaga aaccacaaaa gatacattag
    15661 aagctacatt gattcaaacg agacatagaa taccagaaga aaaacttaaa tctaatcaaa
    15721 tactaatatt tcaagtacca ataccagaaa cccttagaat agtagaacct agtgaagtag
    15781 aaacaagaag aatgcattca gaagaagatt atagtaggat gtgggtatat ctttatgaag
    15841 atatagttag atttaatgat atatctatag cagttgaata tccatgtaaa gtaaatgata
    15901 gatatttaat gaatccatca cctataccta gatttgatat aaaaaaatta aatatgtcag
    15961 acaatttatt cttgtttggt gcaggtagag aaaaaagaat ttatgctatt cctccatata
    16021 caaaggtaga ccctttagag tttgaggatt ataagtttga agaggaaaag tttgaaggaa
    16081 aatactgtag tttatgtaac agtacaaata catttttaga tgaagtatat gacagtgaca
    16141 ctaatgaaaa gtactatagt tgctcagata caagttattg tgaaaaagtt agattaaaaa
    16201 ataacagtat agatgtgaca ataggaggta cttggaatga gtaaaataaa tgactctaaa
    16261 agattagaaa atatagacaa gcaagtatta ataggaagaa atatcaatct tatttatgga
    16321 gatgggtgtg aaaagtgttt tgaaagcaca ggatatgaaa gcaataatat atgtaagtat
    16381 tgtggttcta tagttgcatg taataatgta aatttagaac tttatgaagg agaagtcctt
    16441 ggaattgtag gtgaatctgg ttcaggaaaa agcactttat taaaaatact gtttttccaa
    16501 gaacatgcaa atagtggaga agtatatata tcccattaca ataaagacac aaatatacta
    16561 gagctttcag accaaaaaaa gagatacata aagaatcatt ttatgggtat tgtgtatcaa
    16621 aatccccacc ttggtttaaa tttagacttt agtagtggtg gtaatatagc agaaaaatta
    16681 ttgatggcaa atttatataa tgttgaaaaa ataagagata gaggcaaaga acttttaata
    16741 aaaactaata tacctgtaga aagaatagac cataaaccaa aatactttag tgggggaatg
    16801 caacaacgag ttcaaatagc aaaagctctt tcaaataacc ctccaatatt gctattagat
    16861 gaggttacaa caggtttaga tgtttctgtg caagcggaag tattggattt aataagagaa
    16921 atccaaagag aacttaagat ttctatgata gttgtatccc atgactttga tgttataaag
    16981 atgcttgctg ataggacaat tgtaatgaaa aatggaagtg taattgagtc tggtcttaca
    17041 gaccaaataa tggaggaccc acaacaccca tatactcagc aacttataaa ttcattgcta
    17101 taaatatcgt ttgtaaataa gcaaaatggg ggtttttaat aaaatgaaag aaactatact
    17161 tgaagtaaat aattttagta aagactttga gttacatgca ttgaacaaga ctataacggc
    17221 atgtagtaat ataagttttg aagtttcaaa aggtgagttt ttaggaataa taggtaaatc
    17281 tggagcaggt aagtctacca tattaaaaag tatctataaa acatatatac caacaactgg
    17341 agaaataatt tttaattcag aaatatatgg taatgtagac ctggctaaaa ttggagaccg
    17401 agaaatgata aatcttagaa agaaagaaat aggatatgta tcacagtttt taaagacact
    17461 tccacgaata actgcaattg aactggtagt acattcttta attgagagtg ggtttgaaaa
    17521 agattgttcg tatgatatgg caaaagatat acttaaccaa tttgaaataa aggaaaattt
    17581 atgggatgcg tatccaaata atttttctgg tggagaaaaa ttaagattaa atttagctca
    17641 agctatggtt aaaaagccta gacttttgtt acttgatgaa ccaactgcat cacttgataa
    17701 tcaatctaaa atatatgtaa aagaaaaaat gttggagctt aaaagtcaag gaactacaat
    17761 gataggaatt tttcatgata tagagtttat ggaaactgtt atagataaga cctttactat
    17821 ggcaaaaggg actataagtg aaagtggggt agcctagatg ataaaactaa ttcataatgc
    17881 aaatattgta ttagaagata tgattttaga aaatggtcat ttaatcatag agggagatac
    17941 aataaaaaaa gtatcaaatg ataagattga ttacgcattt gactttaatg agatagatga
    18001 aataatagat gcaaaaaact tatatgtaat acctggtatt atagatattc acagtgatgc
    18061 cattgaaaag gaaatagagc caagaccaag tacattatta ccatttaata tggcatttta
    18121 tgaactagat aaaaaactac ctgcaaatgg aataactaca gtttatcatt ctatatcttt
    18181 aggggatgga gtaggagtta gaagtattga taactcttta aagatgatta aaaatattga
    18241 ttcatataaa aatatagata gtaagagcat aaatcataaa gttcatttaa gatatgaagt
    18301 gttgtattat gaaggtttag aaaaggtttt agaactatta gatgaaaata aaatagacta
    18361 cttatcaata atggaccatt ctccaggaca aggtcagtat acaaatccaa cattttataa
    18421 agagtatgct actaaagtgt ggggagttac agaaaattat gtagatacat ggttagatga
    18481 tttagtaaat ctgcatgata atttagattg gaataaaata gctaatatta taggtatagc
    18541 caaaactaaa aatataaatg ttgcatcaca tgatgatgat acattagaaa aaatggattt
    18601 cataaaaaat ataggcataa atgtatctga atttcctata actcttgaag ttgcaaaaca
    18661 ttcaaagaaa ttgggtgttc taacttgttt aggtgcaccg aatattgtaa gaggtaaatc
    18721 tcataataac aatttaaaag caatggatgc tatattggaa gattgttgtg acattgtgtg
    18781 ttcggattat cttccatcag ctatgataaa gtctatgtgt atagttgcag aaagaataaa
    18841 taatttaaat aaagcagtca gtttatttac ctcaaatcct gctaaagcag ttggtatata
    18901 tgatgaaaga ggtagtataa aagaaaataa aaaagcagat ttagttttag tagatattga
    18961 tagtgaatat ccaaaggttg taaatactat tgtaaatgga aaaacagttt ataaaaggga
    19021 ggtataaaga ttgctaacag agttacattg tcatacaagt atatcagata ataattttac
    19081 tactgaagaa ataataaaaa aagcagcaga aaagaatata aatatcttag caataacaaa
    19141 tcatgacaca ttatctggat tggatgaagc tcttgtgctt ggagagcatt atggaataac
    19201 aataatacca ggtatagaaa tttcagccta tgattataaa aatagaaaaa gagttcatat
    19261 tcttggatat aacatagatt taaacagtca agaaataaaa aatttgtgca atccaatggt
    19321 atgtgacaga cataaagcat ctgttaagat ggtaaataaa attatagatt taggatacaa
    19381 aatatctctg gaagatgtaa agaaatattc atcaaagact ggaatattta aacaacatat
    19441 aatgaaagct cttatagata aaggatatac agataatata tattcaaatc tatataaaga
    19501 attgtttcat agaggaaacg gcaaggtata tgtatcttta aaatatgtgg attacagaga
    19561 tgcaataaaa gctataaaaa attctggtgg aatttgtgtg ttagctcacc cgggtcaaat
    19621 ggataatttt agcgctattg aagagatggt aaaagtagga ttagatggaa tagaagtata
    19681 tcatccatct cacaacaaag aactagaaaa agagtcgctt tcttatgcta agaagtataa
    19741 tctagtggtc acaggaggtt cagattacca cggattttat tctcaacata attatgaact
    19801 tggaagcaag agcttaaata aagaagattt atttaagttt aggttagctt taggaggggt
    19861 attataaatg tcttttttac aagtaaaaaa tgtaggtaaa agttatggac aagttaaggt
    19921 gttaaaagac atatctattg atattgaaaa aggagagttt atttgtttac taggtccaag
    19981 tggttgtgga aagagtacac tacttagaat aattgcaggg cttgaggata aacatggtgg
    20041 aaaaataata ataaatgata aggatatgac taattcacca cctgaaagta gaaattttgg
    20101 tatagttttt caatcatatg cattatttcc aaatatgaat gtttacaaaa acatagcctt
    20161 tggactagaa aataaaaaca taagtaaatc taatattgat aaaaaagtta aagaagttct
    20221 agaagttgta gaattaagtg gatatgagaa aaaatatcca tcacaattat caggtggaca
    20281 acaacaaagg gttgcattag ctcgtgcgat tgctttagag ccagactttt tacttcttga
    20341 tgaaccttta tctgcattag atgcaaaagt aagacttaaa cttagggagc agattagaag
    20401 tttacataga aaactaggca ttacaactat aatggtaacg catgaccaag aagaagctct
    20461 ttgtttagct gataagatgg ttgttatgaa tagaggagaa ataatacaag ttggaacgcc
    20521 aaaggaggta tataaaaatc cagaaacacc ttttgtagct gattttatag gaacaataaa
    20581 ttttatagat gatggtatta ataagatagc tattagacca gaagatataa aagtagagtc
    20641 taatagagat tctaaagata aggatataaa agtgggagaa attttagata tcgaatttag
    20701 aggatttaac tatagaatta cagttgagta tcgttctaag caaatgaaat tagatgtagt
    20761 ttccaaagtt gcagaacaaa tgaaactgtg cataggttct aaaataaact ttaagatacc
    20821 taaagaagga attgtacaat ataaatcaga gggatgtgct taatagctag gggtgaataa
    20881 atgataacat caaaaaataa aagaagaata agtgatagca caatacaaaa atatttcttg
    20941 attttttcgg taattgtact aatagtattt atattatttc cattagtatc attattaaaa
    21001 aatgcattta tggatacaag tggaaatttt ataggaattt caaactttag taaatatgtt
    21061 gaaaatccaa gtcttatagc atcatttaaa aattcaattt ttgtatctac agtctcatct
    21121 ttaatatcac tatgtttagc atttatatat tcatacgcaa taacaagaac aaacataaag
    21181 tttaaagatt tttttaaaac tatgggaatg tttccacttt ttgcaccaac tatgttatat
    21241 ggtatttcac ttatatacct ttttggaaat aagggaatct ttacaaccat gggtctggaa
    21301 gtaccactat atggtccatt aggaataatt atttctcaga ttctatttac atttccacaa
    21361 gcttttttaa tattatctgt agcactttcg atggctgatt atagacttta tgaagcagca
    21421 gatagcttgg gtgctagtga atttaagaaa ttttgcaaaa taacaattcc tggtataaag
    21481 tactcattac ttagttcatt ctttgtatcc tttatacttg catttacaga ttttggagca
    21541 gctaaggtag tcggaggaaa ctacaatgta ctggctactg acatatataa acaagtagtt
    21601 ggacaattta acataccaat gggagctact gtttctatgg taatgttaat tcctgtacta
    21661 atagcattta cattagataa gatggcaagt aagaaacaag gtatgaccat aactgcaaag
    21721 tctatgccat ataagattaa ggaaaataag tttagagata gagtattttt aatagtatgt
    21781 agttttataa cattgctaat ttttatttta atattcgttt cagttatagg ttcagttata
    21841 aagttatggc catataattt aagctttaca atggaacact atacatttaa aggtattttg
    21901 ggaagtggaa taaaaacata cttaagcagt ttgaaaatat caactttaac agcaatattt
    21961 ggaactatat ttgtattttt tagtgcttat atgatagaaa aggtggataa gtttcctaaa
    22021 ttaagacaaa cagcttacct tttatcaatg ataccaatgg ccttacctgg cttagtttta
    22081 ggtatatcat atataacttt ttttaactca gcaagtaatc cattaaattt tttatatgga
    22141 agtacagcaa tattagttat agtaaatgta gttcattttt attcagtttg ttttataacc
    22201 tcaaattcat ctttaaaaat gttggataaa gagtatgaat tagttgcaaa atccataaat
    22261 attccattct ataaagtatt ttttaatata acagttccaa tgtctataac atcaatactt
    22321 gaaatagtag tatattattt tgtaaattca atggttactg tttcagcatt aatattttta
    22381 tatacacctc aaactcagac tgcatcaata tcaatactaa agttagatga gataggatat
    22441 ataggaccat cagctgctat ggcagtatta gtgcttttaa caaatataat agtaagactt
    22501 ttgtacgaat ttgtaacaaa gaaattaaaa aataacacac aaaaatggca gcaaaaagat
    22561 gttgcatagg ggaggtaaaa atgaaattta agaagctagt ttctttagga gtattatcaa
    22621 cacttttatt aagtgtaata acaggatgta gtactgattc atcaggttca gattcaaagt
    22681 ctgatagtaa gacaaaaggg gatttaacag tttacacagc aattgaggaa gatagtatag
    22741 agccatattt agctacattt aaagagaaat atccagatat taaattaaat attgttaggg
    22801 catcaacagg agatataaca gcaagattat tagcagaaaa ggataatcct caagcagatg
    22861 ttgtatgggg agttgcagcc acaagcttat tggttgcaga tgaccagggg atgttagaac
    22921 catatgctcc aaaaggttct gaagaaatag agtctgcatt taaagatgat aaagaagttc
    22981 cttcttgggt tgggatagat gcttggatga caggtataac agtaaacact aaagaattaa
    23041 gtgataagaa catcccagtt cctagttctt acgaagattt aataaagcca gaatataaag
    23101 gtcttatatc tatgcctaat ccatcttctt ctggtacagg gtacttaaca gtatcagccc
    23161 ttatacaaat tatgggtgaa gaaaaagcat ggcagtatat ggataaattg catgagaata
    23221 ttggtgtata cactcaatct ggttcagctc cagctacatc agcagcttca ggagaatatc
    23281 ctattggtat atcttttgga tatagaggta taaagttaaa agaggaaggc tatccagtag
    23341 aagtagtatt tccaaaagaa ggttctggat gggatataga agcaaatgct cttgtgaaaa
    23401 aagataatat aaaagaagca tcaaaagtat ttttagattg ggctataagt aaagatgcaa
    23461 tgaacgaata ctctaagaac tatgcagtta ctactatttc tacaggaaat ccaataccag
    23521 aaggattccc taaaaagcca ttagagcaaa tgatagataa tgatttaaaa tcagcagcaa
    23581 aaaatagaga agatatacta aataaatgga tatcaaaata tgatggtaag acagaaaaag
    23641 agagttaagt aaaactatac aaatggagta aaaagaaaga atttttttaa gtagatttta
    23701 ttataataat ataagttgag aataatatag gtcaagaaaa gtgaatattc tataaaaatg
    23761 tatagagtaa gtataccatt cagtttgaat ggtatatttt ttatttttag agattaattt
    23821 agaaatagat atactacagt ttaaaatagt atattttaat ttcattatta aagtatagat
    23881 attatatata aagtaaataa attattgagc gattattaat aagatatgta taaattttaa
    23941 tttgggaact tgtgttatat atcatgaaaa aagagaaaaa ataaaaggta aattgtttct
    24001 tgataacttg aaattggaag tgagaatatt taactagaga agaattattt aagtttaagt
    24061 cagctttagg aggaggaatt ataaatgtct tttttacaag caaaaaatgt aggtaaaagt
    24121 tatggacaag ttaaggtgct aaaagacata tctattgata ttgaaaaagg ggagtttatt
    24181 tgcttattaa gtccaagtgg ttgtggtaaa agtacattgc ttagaataat tgcaggactt
    24241 gaggataaac atgatggaaa aataataata aatgataagg atatgactaa ctcaccacct
    24301 gaaagtagaa actttggtat tgttttccaa tcatatgcac tatttccaaa tatgaatgta
    24361 tataaaaata tagcctttgg attagaaaat aaaaatataa gtaaatctga tatagataag
    24421 aaggttagag atattttaga agttgtagaa ttaagtggat atgataaaaa atatccatca
    24481 caattatcag gtggacaaca acaaagggtt gcactagctc gtgcgattgc tttagagcca
    24541 gactttttgc ttcttgatga acctttatct gcattagatg caaaagtaag acttaaactt
    24601 agggaacaga ttaaaagttt acatagaaaa ctaggcatca caactataat ggtaacacat
    24661 gaccaagagg aagctctttg tttagctgat aaaatggttg ttatgaataa tggagaggta
    24721 atacagattg gaactcctaa agaagtctat aaaagtccaa aaacaccttt tgtagctgat
    24781 tttataggaa caataaattt tatagatgat gatgtgaata tgatagcaat tagaccagaa
    24841 gatataaagg tagaatttaa tagagacttt aaagaaattg atgtaaaagt aggagagatt
    24901 ttggatatag aatttagagg attcaactat agaattacag ttgaatattg ttctaagcaa
    24961 ataaagttgg acgtagtttc aaaggtagtg gaacaaatga aactatgtgt aggttctaaa
    25021 ataaacttca agatacctaa agaaagaacc atacaatata aatcagaggg atgtgcttaa
    25081 tagctagagg tggataaatg acaacaataa aaaataaaag aagaataagt gatagtaaga
    25141 tacaaaaatg ttttttagtt tttttagtga ttgcattatt agtatttata ttatttccat
    25201 taatatcact attaaaaaat gcatttatgg atacaagtgg aagttttgta ggaatttcaa
    25261 actttagtaa atatattgaa aatcccactc ttatagcatc atttaaaaac tcaatttttg
    25321 tatctacaat ttcatcctta atatcactat gtttagcgtt tatatattca tatgcaataa
    25381 caagaacgaa tataaggttt aaaaactttt ttaaaactat gggcatattt ccgcttttcg
    25441 caccagctat gctatatggt atttcactta tatacctttt tggaaacaag ggaatcttta
    25501 caattatagg catagaagta ccactatatg gtccattagg aataattatt tgtcaaatac
    25561 tgtttacatt tccacaggcc tttttaatat tatctgtagc actttcgatg gctgattata
    25621 gactttatga agcagcagat agcttagggg ctagtgaatt taagaagttc ttcaaaataa
    25681 caattcctgg tataaagtat tcattagtta gttcgttctt tgtatctttt atacttgcat
    25741 ttacagattt tggagcagca aagatagttg gaggaaacta caatgtattg gctactgaca
    25801 tatataaaca agtggtaggt caatttaaca taccaatggg agctactgtc tctatggtaa
    25861 tgttaattcc tgtactaata gcatttacag tagataagat agcaagtaaa aagcaaggga
    25921 tgactataac tgcaaagtct atgccatata agattaagga aaataagttt agagatagag
    25981 tatttttaat agcatgtagt tttataactt tattaatctt tattttgata tttgtttcag
    26041 ttataggctc agttataaaa ttatggccat atgatttaag ctttacaatg gaacactata
    26101 cgtttaaagg tatatcagga aatggaatca aaacatattt aagcagttta aaaatatcaa
    26161 ttttaacagc aatatttgga actatatttg tattttttag tgcttatatg atagaaaaag
    26221 tggataagtt tcctagactg agacaaatat cttatttttt atcaatgata ccaatggcat
    26281 tacctggttt ggctttaggt atatcgtata taactttttt caattcagca agtaatccat
    26341 taaatttttt atatggaagt acaacaataa tggttatagc caatatagtt catttttatt
    26401 cagtttgctt tataacttca aattcatctt taaaaatgtt ggatagagag tatgaattag
    26461 ttgcaaaatc tataaatatt ccattttata aggtgttttt ttatgtaaca cttccaatgt
    26521 ccataacatc aatacttgaa gtggctgtat attacttcgt aaattcaatg gttactgttt
    26581 cagcattaat atttttatat atacctcaaa ctcaaactgc atcaatatcg atactaaagt
    26641 tagaagagat agggtatata gggccatcag ctgctatggc agtattagtg tttttaacaa
    26701 atataatagt aaaacttttg tatgaattga caacaaagaa attaaaaact aagacacaaa
    26761 aatggcaaca aaaagaagtt gcatagagga ggtaaaaatg aaatttagga aattagtctc
    26821 tttaggaata ttatcaacac ttttattgag tgtaataact ggatgtaata atgcttcatc
    26881 aggttcaaat ttaaagtcta ataataagat aaaaggagag ttaacagttt atacagcaat
    26941 tgaagaagat agtatagata catatttaac tacatttaaa gagaaatatc cagatattaa
    27001 attgaatgta cttagattat cagtaggaga tataacagca agattattag cagaaaaaga
    27061 taatcctaaa gcagatgtgt tatggggggt tgcagctaca agtttgataa ttttagatga
    27121 caaagaaatg ctagagccat acgctccgaa aggctttgat aaggtaaatc ctgtatttaa
    27181 agatgataag aaagttcctt cttgggttgg aatagatgct tggatgactg gtataacagt
    27241 aaatactaaa gaattaagtg ataagaacat cccagttcct agttcttatg aagatttaat
    27301 aaagccagaa tataaaggtc ttatatctat gcctaatcca gcttcttctg gaacaggata
    27361 tttaacagta tcagaactta tacaaattat gggtgaagaa aaagcatggc agtatatgga
    27421 taaattgcat aaaaatattg gtgtatacac tcagtctggt tcagctccag ctatattagc
    27481 agcttcagga gaatatccca ttggtatatc ttttggaaat agaggtataa agttaaaagg
    27541 tgaaggttat ccagtagaag tagtatttcc aaaagaaggt tctggatggg agatagaatc
    27601 aaatgcactt gtgaaaaaag ataatataaa agaagcatca aaagtatttt tagattgggc
    27661 tataagtaaa gatgcaatga atgagtattc taagaatttt gcagttacta caattgacac
    27721 gggaaatccg gtgccagaag gatttccaaa gaatccatta gaacagatga tagataatga
    27781 tttaaaatca gcagcaagaa atagagaggc tatactaaat aaatggatag aaaaatatga
    27841 tggaaagaca gaaaaagaga gctaagcctt gcaataaaat ttagtacaaa aagcgcctag
    27901 aatataagtt ttatatctag gctcttttga tatatttatt taaaatgcat atttctatat
    27961 aaaaataaat agtttagtac cttgattaaa ttaaataaaa accttaaaat aaagagatgt
    28021 aaagaaaaac taaaaacgaa taaaaggtga atttatatga taaaagaaat aatagtagta
    28081 gagggtagag atgacgttac agcagttaag agagcaatag atgcagaact tataactaca
    28141 ggtggatttg gatttccaaa aggtgtaatg gagcgtataa aagctgctaa tgaaagacgt
    28201 ggtgttataa tatttacaga cccagatttt gcaggtgaaa aaataagaaa aaaaatagca
    28261 tcagaagttc caggatgtaa acatgcattt ctaccgagag aggaagctaa aaaggatgga
    28321 gatataggta ttgagaatgc tactcctcaa agtataatag cagctctgaa taaagttaga
    28381 actgaaaata cagaaaaaag agatgaattt aagcaagttg acttaattag aaatggtctt
    28441 ataggtaatg aagaagcatc acttagaaga gatgcacttg gaaaaatact tggaattgga
    28501 tatggaaatg caaaacaatt tttaaataga ttgaataatt atggagttga aagagaagaa
    28561 tttatagatg cattaaaaca aatctaaatg taatgtttaa tgtatgaaaa atatgtatat
    28621 aaaataaatt gtggaattac ggttaattta agataaatta tttataatat ataccttatt
    28681 ttaaatcaat gtaaatacat ataaataagt ataattaata aaattaaata tcaaaataag
    28741 ttaaaaagga gtaaataatg gatagactat cttcacataa tgcaacaaag gaagtagtac
    28801 aaaaatataa ttttaaattt tcaaagtctt taggtcaaaa tttcttgata gatagtaata
    28861 taattgataa aatcttatct ggagctagaa tcacaagagg agataatatc atagaaatag
    28921 gacctggtat aggaactctt actcgtgaga tgggtaaaat agcagaaaaa gtagtggcta
    28981 tagaaatcga tagaaattta attcctatat tgaaagatac cttgtcagat ttagataata
    29041 cagaggttgt aaatcaagat atactaaaag tagacataca ggaattagta aaagataaat
    29101 taaatggtgg gccagttaag cttgtagcaa atcttccata ttatataact actccaatag
    29161 ttatgaaatt tttagaggaa gacatacctg taacagacat agtagtcatg gttcaaaaag
    29221 aagttgcaga tagaatgaat gctattccgg gtactaaaga ttatggggca ttatctatag
    29281 ctgttcaata ctattgtgat acagagattg tggcaaaagc tccaagacat atgtttatac
    29341 cacaaccaaa tgtagattcc acagttatag gtttacatgt aagagataaa agaaaatatg
    29401 atgttcataa tgaagacata ttttttaaaa cagtaaaagc atcctttgga caaagaagaa
    29461 aaactcttct taattcgttg ggaggtcttg gttttcttaa taaagatgaa ataagagaaa
    29521 tattaaaaga agctaatata gacgaaaaaa gaaggggtga aactcttagt atagaagagt
    29581 tttcagttct ttcaaatata ataaatacaa aagtatcttc taaatagaag gtactttttt
    29641 taattttgtt aacatatatt gtttagagaa actgtgttaa ggggggattg cagtgggttc
    29701 taaaagaaag tttaaaagag actatataat actagaagct aaagatataa actttagata
    29761 caaagaacga gtactgccaa aagcttttgc aaaagttgaa gtaaatgatg aaaaagcaat
    29821 tattgcatta tatgtagaaa atctaaaata tgttaaagat gggtataaag cagtagcaat
    29881 aaaagatgac catggattaa tagacttagg aaatataatt ttaaatgaac aaggaagagg
    29941 tgaatttgtt ttagatttag aagattatga tgatgagata aaaggtattg cattattgta
    30001 tgaaagaaat atacctttaa ttggatttaa gggaaataag cttgaaaatt atgaggacat
    30061 attatttcaa gaggtagagg aagaagaata tgaagaatat gaagagttgg aagaagaaga
    30121 cgaagagata gaggataatg aggaaagtga tagagacgaa gatagtgaag aagaatatat
    30181 tatagaagaa tatgatgaag atgagtatga agactattgg gaagatgatg aagatagtga
    30241 agaaggagag ttaaaagcaa gaacggaagt agaagattct ggtgatgagg aaatagaaga
    30301 gttcaataat tataaggaaa tagaaaatta taatagggtt aaagtacagg aagaatatat
    30361 agacatagaa tatgatgagg aagaagagga agaagaagtt tttgaagtaa atagagaaga
    30421 agataggttt gtttcctcaa aacctactaa atcaaagaaa gttcaaaaag atcagaataa
    30481 tattgaaaaa ataaaaagag ctaatggagt aagtcaagga gagagtggaa ataaacaaac
    30541 cagtacatta cttatgccaa gacaaataaa aaaaggtttg aaattattta aagaagtaaa
    30601 accttttgta acagattata tagataagac aagatggtgg aaaatagaga taaatcctac
    30661 aacactgtgt ggatatacaa tgccttattt aggatattta aacgtattga attacactat
    30721 gtatagtgat gcagttttac attcatataa atacagacac tatttatttg gagttcagta
    30781 tgatgaatat aataagagaa aacattatat ttatgctatc ccaggaaata gaaatgaaca
    30841 gccagacaat ggaagcacag gatttttaag atatcaagct tgtgataata gaacaaatag
    30901 tttaggatat tggctatgtt ttatagattc tagacaaaga aatatagtta ggtaaaaata
    30961 ataaaaatga aaaaatttag actcaattct tattttactg aaaaaattaa tgaaatgtag
    31021 tacaaaactt taaaaactat tgataatatt taaatattac actacaatat atcttgaaat
    31081 acctattata ttatggagga aaattatgat aaacgagcaa agaattttaa atgagtttct
    31141 tgaattagta cagatagata gtttatcttt aaaagagggg aatgttgcta aagtattagt
    31201 taaaaaatta gaagaaatag gttgttcagt agtaatagac aatgctggtg aaaaagcaaa
    31261 tggagaaact ggtaatataa tagctacatt aaaaggaaat aaagaaggaa agaaaatatt
    31321 atttagttca catatggata cagtaactcc aggtataggg gtaaaaccta tagtggatga
    31381 agcaaatgga ataataaaaa gtgatggtac tacaatatta ggttctgatg ataaagcagg
    31441 tatagcagct atattagaag gcttaagata tataaatgaa aataacatag aacatactga
    31501 tatacaagtt gtattctcta tatgtgagga atgtggatta gttggagcta aaaatttaga
    31561 ctatgaaaaa atagattctg aatatgcatt tatattagat agtggtggtt ctccaggtga
    31621 aataatagta aaagctcctg cacaagatgt aataaatgtt aagatattag gaaagactgc
    31681 tcatgcaggt cttgaacctg aagctggaat aagtgctata atggtagctg ccagagcaat
    31741 agaaaatatg aatttactta gaatagacga agaaacaact gctaacatag gaattataaa
    31801 cggtggaaca gctacaaata tagtaacagg agaagttaat ataattgcag aagccagaag
    31861 tctaaaagaa aataaattag atgttcaaac taaacacatg gtagagactt ttgaaaaagc
    31921 tgcaaaagat tttggagctc aaattgagat agatgtcaat agagcatata caccaataga
    31981 tgttagtgag gactctgaaa taataaaact tgcaaagaag gctttttcaa acttaggtat
    32041 agaaggtcat actgaatcta caggaggagg tagtgataca aatatattaa gtaagaatgg
    32101 tatcgaagct ataactttag gaattggtat gaaaaatgct catacactaa gtgaacatat
    32161 agctataaaa gacttatatg attctgctaa aatggtagtg gagataataa aggaagcata
    32221 aatttaaata atagtgtata atatatatat cgcctagctt aagctaggtg atttttcatt
    32281 ttatagaaga ttgataatct ctattgaaag caaagagtat acatttgcat ataagagagt
    32341 gtttaattta tgttcaaatg taggttctta ataatattta gattgtttca taagggagga
    32401 ataaatatgg aaacaaggta tgaagaagca aataaaataa caatacaatc aatactttgg
    32461 aatgtagtat taacaataat taaggttata gctggtgtta ttggtaattc tagcgctatg
    32521 attgcggatg gattacattc agcatcagat attataagtt ctataggagt actaataggc
    32581 aattatgtat cttctagacc tggagataga gagcataatt atggtcatga gaaagcagag
    32641 acattagtat catttgtgtt atcaatattg cttattttcg tttctataac aataggtata
    32701 gaggcaatta aatctttatt taacttagat gcattaagtg ttccatcaat attaccatta
    32761 gtagtatcag ttatttcaat cctgataaaa gagtatcaat atagaataac aataaaagtt
    32821 gctaaaaaga taaattcacc agccctaaaa gctgatgctt ggcatcatag gtcagatgct
    32881 ttatcttcag tagctgcttt tataggtata ggtggttcta ttttagggtt taaaccatta
    32941 gacccaattg cgtcagttgt agtggctata tttgtagcta aagtaggaat tagtatactt
    33001 ataagttctg ttaatgaatt gatggatgtt tcagttgatg aagaagagat aaaagaatta
    33061 aaatttattg tagcagatac agaaggtgtt aaaaatcttg gtgatataaa gactagaaaa
    33121 catggagcta tggcatatgt agatttgact atatgtgtgg atgaaaattt gacagttaag
    33181 caaggtcatg atattgctac caagcttgaa aaacatataa taaaacatat ggaatttgta
    33241 aaaggcataa cagttcacgt tgaaccatgt actaattgcc aaggaaataa gtgtaataat
    33301 taattaaaac ttaaaaaaaa gtgtaaaaag ttatataatt gttttatgat tataaactct
    33361 aggggtggtt aaatgagcaa gaaaagaaaa ctaaagaaaa aagctgcaat attaatagga
    33421 gtagttgttt tctttataat atactttata tcctttagaa taggaataaa tatggaaaag
    33481 gataaaagtg aagtgccaaa gaaggtagaa aaagaagaag aagataatag gtctttaagg
    33541 actcaaataa aagagaagaa gaaggtttat atatcagata aaaatataga aaacataaaa
    33601 attgaagaag atatgtggga tgaaataaag ttatttttct cagattttaa gaaagtaaga
    33661 aatactactt cttcttacga agcagtttat gaaggttatt ctgatgatgg agttaaattt
    33721 tctactgacc ttaacttttt tagagtatat actgtaaata aagaagaata ttataaagta
    33781 ccagttagtt ctaaggatgc ttttaagaaa cttttagatg agagtatata tacttcgttt
    33841 gaccttttaa gtcattataa agattggaaa gaggttacta taactcatgg aaaagaaatt
    33901 aaaaaagttc atagatggaa atttgatgat ttagcacata agatgatttc aaagagggtt
    33961 gtaggaaaag ttcaacctga gaaaagtaag gaaagaagtg actataattt tactataaat
    34021 ataaaaggtg ataattttga tgtaaaagtt gatacaatgg gaaaagatta tgtaaaagtt
    34081 acttctaaga aaaatgtatc ttactatgag gttcatacaa gtttatttga ttaccttaaa
    34141 aatgagatat ttgaaattgg aaagaaataa gactttaaga aggctatcaa attagatatt
    34201 tgatagccta aattatttta tatattattt ttcaaccaac aagtctgcaa ccttataaat
    34261 tggaccagca ccagcagtta ttactaaatc attatcctta acattttcac gtaagtattt
    34321 tactatatct tcaaattctt ttatatagat ggcatctaca ttgttttgat ataatttctc
    34381 aaccaagtcc ttagaatgta tatctcctgg gtctttttcc ctagcagcat atatatctgt
    34441 tatgataact ttatcagcag catagaatgc ctctgaaaat tcacctaaaa gagattttgt
    34501 tctagtataa gtatgtggct ggaagataca ccataaagtt gattttttta attttttggc
    34561 agcagctaaa gttgctttta actcagttgg atgatgtgca taatcatcta ctactaaagc
    34621 attcttatag taccctttag tttcaaatct tcttccgaca cctttataaa tttttatatt
    34681 ttttcttata gtctctaagt caataccaga aacaagagct gccattatgg cagaagaagc
    34741 attgtatatg ttatgaagac catatacaga aagttcaaat tctcctaaat ctcttccttc
    34801 atattctatt ctaaatattc catgaccact attgtcaaag tgaatatctt ttattacagc
    34861 atcattgtca gaatctctgc cgtattttat aatagtagct ttaacatcat acaatatgtc
    34921 atcaatattt tcatcatcgc catttattat gaaatagcct tctgggggta ataattttcc
    34981 aaatttgtta aaagaagcct tgatttcatc tattccagag aaataatcaa gatggtcttc
    35041 ttcaacattt aaaactatag atatctttgg gttaaaatta aggaaactat ctacatattc
    35101 acatgcttca gttataaagt ggttggaatt acctatctta acattaccac ctatcatact
    35161 taagttacca ccaactagta tagttgggtc taagtctgca tattcaaaga ttgtagataa
    35221 catagatgtt gtagaagttt ttccatgtgt tccagaaact gctatagaat ttttatattc
    35281 cctcatgatt tggcctaaaa aagttgctct gttcattacc aatttatttt tttctttagc
    35341 agccacaagt tcttcattgt ctggatgaac tgctgctgta taaacaacca tatttacatc
    35401 atctgatata ttatcttttt tctgaccaat ataaatagtt gccccttggt ccctaagttt
    35461 gtctaatagg tatgattcgt ttgaatccga acctgaaact tgataacctt tattgataca
    35521 tatttcagct aatgcactca tgctgatacc gccaattcct ataaagtgta tattcataat
    35581 aaaaccacct ttctttttaa ttaagtccta caatatgaac tatttctgtg gtataataat
    35641 ataatgttca atattttata attaatatgc atataatatt atatgtattt tatttttgaa
    35701 tataataaaa cataagtaat attataccac aataagaatt gttatgcatt agttagttta
    35761 acgttatata aaggtacttg taggaggaaa taataatgaa atttaaaaga acagagagaa
    35821 ttggagcaat agttaaaata ctgtctgata atccaaataa aatatatact ttaagttatt
    35881 ttacaaatca atttaatgca gcaaaatcaa caataagtga agatttatta gttgtaaaaa
    35941 atgtatttga gaagctacat ttaggtaaag ttataactat ttcaggagca gcaggaggag
    36001 taaaatatat acctaaaact tctatagcag aaaatgcaga gttcttaatg gaattatgcg
    36061 agagaatatg tgacaagtct agaatactat ctggtggttt tttatacttg attgacctaa
    36121 tttacgaccc tacaatagct gctaagatag gaaagatatt cgcatcaaat attgagtatg
    36181 tagatgcaga ttatgttgtg acaatggaaa caaaaggtat acctatggca cttatgactg
    36241 caaaagctat gaatttacct ctagttataa taagaaaaga tataaaggta tcagaaggtc
    36301 ctacacttag catgacatat gtgagtggaa acagttctaa agttgaaagt atgagtttgc
    36361 caagaaaagc tcttaaacca gatagtaaag ttataattat agatgatttc atgaggggtg
    36421 gaggtactat aaaaggaatg gtagacctta tgaatgaatt tggtgcagag gttataggta
    36481 ctggagtatt catatctaca acgaatcctt cagaaaaaat ggtaaaagat tatatttcat
    36541 tgattcaatt ggatgttaat ggagataaaa tagtagtaga accaaactta aagactttta
    36601 aagatgaata tagaaatgaa gatttagacg aagaagaaga ttcagaattt gaatttgaga
    36661 tagatgaaga ttaataaaaa aaatatttag aaaaatatgt aaaaataaaa agaggatatc
    36721 cctagttgtt catagaatta tttaaaaaac agtttatatt aatttattcc agcgataata
    36781 attttaatat aaattaattt ctgtcaaaat caaaggggga tattttggta tgaagataac
    36841 tgacgtaaga gtaagaaaat taacagaaga aggtaagatg aaatgtatag tttcaataac
    36901 tttcgataat ttgtttgtag tgcatgatat aaaagttata gaaggccata atgggctatt
    36961 tatagcaatg cctagcagaa aagtaggcga aggaaatttc agagatatag ctcatccaat
    37021 aaatgcagaa atgagacaag ttttagaaga tgcagtttta caagcgtatc atgaagcatt
    37081 agttcaatgg gaagtagctg ctgaataata ataaatatat aaaatggaga gagcccgaac
    37141 atttaggctc tttttaaatg tagaatttct attaaaatat gtacttttta gtaaaaaagt
    37201 gctatattat tagtgataat gtatatgttg tatacaaatt gttagataaa tgtattataa
    37261 tgtatattga ttaagagaat aatacgaata aaattaaatt agattgaatg gagtgtatca
    37321 atgaacttta aagctataat ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac
    37381 ccaaaagtta tacataaagt atgtggaaaa gaaatggtta accatattat agatgtttca
    37441 aaaaaatcag gggtaaaaga tactgtcgtg attttaggac atgaagctga tgtagttaaa
    37501 gaaaagcttg cagaagaaat tataatagcc atgcaaacag aacaacttgg gacaggacat
    37561 gctgtaaaga tggctaaaga atatattaat gatgaagata caatagttgt actttgtgga
    37621 gatactccac ttataaaaga agagacacta aagagactat ttgaatatca tatagaaaat
    37681 aaatatcatg ctacagttct tacaacaaga gttggaaatc caacaggtta tggtagaata
    37741 ataagagata agaagggtga ccttcttaaa atagtggaac aaaaagatgc aaattcagaa
    37801 gaaaagatga taagtgagat aaactctgga atttattgtt ttaatggaaa gagtcttaga
    37861 gaagcactag atttgctaaa taataacaat tcacaaggtg aatattattt gactgatact
    37921 gctaaaataa tgagagataa aggattaaaa gttggtgcat ttgcaggttc aactattgaa
    37981 gaacttatgg gcgttaactc aagagtagaa ttatcaaagg cagaagaaat tatgagacga
    38041 agaataaatg aatctcatat ggtaaatgga gtaactataa tagacactaa ctctacctat
    38101 atagaatctg atgttatgat aggtaatgat actataatat atccaggtgt tatgttacaa
    38161 ggaaaaacaa ggataggttc agattgtata attggaatga attcatctat aacaaattca
    38221 gaaataggtg atggtacaga aataaaaaat tcaactataa ttgatagtaa ggttggagaa
    38281 aattctactg ttgggccata tgcatactta agaccaaaga gtgacttagg aaataatgta
    38341 aaaataggcg attttgtaga agtaaaaaat gctattatag aagatggctc aaaagcatct
    38401 cacctttctt atataggaga tgcacatgtc ggtaaaaatg taaatattgg atgtggagta
    38461 gtatttgtaa actatgatgg taaaaataaa tttaaatcaa tagtaaaaga caatgcgttt
    38521 ataggttcaa attctaattt agttgcacca gtagttgtag aagaaaaagg atatatagca
    38581 acaggttcaa caataacata tgatgtccca gatggtgctt tggcaatagc aagagaaaga
    38641 caagttatca aagaaggatg ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa
    38701 ttatgtagtt tattattttg gctaaaaaat ttagctcgaa attattataa attatattca
    38761 ttagtatttt aaaattttca ggagggttac ataaatgaat actagcggaa gcgagattaa
    38821 aattattgca ggtaattcat cgaaagagtt agcacaaaaa atagctgact atataggtgt
    38881 atcagtacta gattgtgaag taggtacatt tagtgatggt gaaatatgtg taaacatgaa
    38941 tgagacagta agaggttgtg atgtgtttgt agtgcaatct actaacagcc cagtaaatga
    39001 taacttaatg gagttattaa tcttaattga tgcgttaaag agagcatcag caggtagaat
    39061 aacagcagtt attccttact atggatatgc aagacaagac agaaaggcta aggcaagaga
    39121 tccaatcact gctaaattag ttgcaaattt aataactgct gctggagcag atagagtatt
    39181 aacaatggat ttacatgcag ctcaaataca aggatatttt gatataccat tagaccactt
    39241 actaggtgga acaatattag caaattattt taatgaaaag aaaatagaag atttagtagt
    39301 agtatctcct gacttaggaa gtgttactag atcaagaaaa tttgctaata ctttaaatgg
    39361 agaagttcca atagctataa tagataaaag aagaccgaaa gctaatgtat gcgaagttat
    39421 gaacttaata ggtgatgtta aaggtaaaaa tgttatacta ttagacgata tgatagatac
    39481 agcaggaaca atagttaatg ctgcaaatgc acttaaagag tttggagcaa aagatgttta
    39541 tgcttgttgt actcatggtg tgttatcagg tccagctatt gaaagaattg ctaattcaga
    39601 aataagtgag ttaatagttc ttgatactat acaacttcct gaagagaaaa gaatagataa
    39661 gataaagata aaaacagtag ctcctttatt tggagatgct ataagaatga tattctctaa
    39721 tgaatcagta agtaaattat tctaatttat tgtatttatg agagttgttt aaaaggatat
    39781 aaaccttttg agcaactctt ttttgatttt ataattatgg aatgttattt ttttcttgtt
    39841 agaaaggtat atattattgt aaaaagagat tatagtttaa aattatgtat atttaagtaa
    39901 aataaactta ataaagggca tattattcaa aatattatga atataaaaaa tattacaaaa
    39961 aagtattgaa aaaattcaga ttatgtagta ttatgtaaat ataaataaag aaagaggtgt
    40021 ttccaatggg aaggttagaa gatgaacccc taataattaa atgattcagt cacaatctaa
    40081 agtgaatata aaaatctatt gaaagtttat ttataagaat tgaaaatagt atctattcta
    40141 tctttagtgt ttgtgagagg gttctggatt atataagatt actgtaaaga agtaattttg
    40201 cacaataagc atatctgtaa atgaagagtt tcgtttatat aatgattgtg gctgaatcat
    40261 aactataata gatagaatgt ttataataga tgattttata aaatactata ttgtaatagc
    40321 ttttagcttt caaataaata ttagcaaagg atggaaatga caatgataaa actaaatgat
    40381 aatttataaa tatctcctta cgatatataa taattgtagg gagatgtttc tttttttgta
    40441 tagcataatt tttctagaaa ttctgtttca atatgtaaaa agttggtata tataaatagt
    40501 ataaacagtg ttgctaaaat atggtagata ttatataata taagtggata tgtatataca
    40561 ttatgagtta tataatttga atttaataaa atagaaacgt tgatttatgt tctgtaatgt
    40621 gggcaccttg gagcatattg agttagtggt gcaaccggct atgaaattgt atttatttat
    40681 aaatactatt attttcatag ctttttttat ttaaaaaatg gttatatgtt agaaataaat
    40741 ttggttagta cgtttaaata attttactta gatttttaac cagatatatt gtaagtaagt
    40801 attaattaaa aaatttaagg gggaataaaa atgaagttaa aaaagaataa aaaaggtttc
    40861 actttagtgg aattattggt agtaattgca attataggta tattagcagt agtggcagtt
    40921 ccagctttat ttagtaatat aaacaaggct aaggtagcaa gtgttgagtc tgattatagc
    40981 tcagttaaga gtgcagcatt atcttattat tcagatacta ataagatacc agttacacca
    41041 gatggtcaaa ctggtttaag tgttttagaa acttatatgg agtctcttcc tgataaagct
    41101 gatataggtg gagaatataa attgattaaa gttggtagta aattggtatt acagataggt
    41161 acaaatactg agggagttac cttaacagaa gcacaatcag caaaattatt gagtgatata
    41221 ggtgaaaata aaatatatac aaatgcagct cttagtgcta aattaacatc tactacaaag
    41281 gtaaataatg aagctttata tatagttctt atagataata ttgtaatgga tcaacaagga
    41341 gcttaaaagt aaattgttat aatgaaaaat ataataaaaa aacaggaaga aaatcttcct
    41401 gtttttttat actattttct ttaatgttca attatgaatg atgatataat tgagatagta
    41461 aaggagtgaa acctgtggct aaaaaagtta gaataggaga caaactggtc gaaaaaggat
    41521 atataacaga agaacagctt aaatgggcac tatcagaaca aaaaaacagt gggaaaagac
    41581 taggagaatt tctagtacaa gaagggctta tagatagtaa cctactaata agtgtattaa
    41641 aagaactctt ggatatagaa agtatatttt tagaagggac agaaatagac actttagcta
    41701 caaagatggt tccagaaaat atatgcaaaa gatatactgt atttccattc aaaatagatg
    41761 gaaataaaat atgtctagcc atgtcagacc cacaagacag agaagcagtt caagatgtaa
    41821 ggcgtatgtc aggtaaagac gtagagatat ttatatcaag tacagaagat ataaacaaag
    41881 caataggtca tgcttatgca cattcagaaa taaataaagc aatgactgag tacaacaaaa
    41941 atagaactgg tggagtaaga gaaacagtaa tacttgaaga agatgtaaat gctgctccaa
    42001 tagtacgatt agtaaataac atactagaaa atgctgttag aatggaagca agtgatatac
    42061 atatagaaca aagtgaaaat tacatgcgtg ttaggtttag gatagatgga atgttaagag
    42121 aatatatgag gatgaactca gcaccatata aggcagtaat cagccgtatc aagataatgt
    42181 cagatataaa tatatctgaa aagagaatac cacaggatgg tagaatctat ttaaaagtgg
    42241 ataataaacc catagatttt agggtttcta caatgccaac taacagggat gaaaagatag
    42301 ctatgagggt tcttgataag agtaatttta tggttagtaa agaagtacta ggaatagacg
    42361 aacatggctc taagatatat gatgaattga taaacacacc ttatggtctt atattggttg
    42421 ttggccctac tggaagtgga aaaaccacta cattgtattc aatgttaaat caactaaata
    42481 cagaaaatag aaatctatta acaatagaag accctataga gtatgaatta cctggtgtaa
    42541 atcaatctca aataaatgaa aaggcagggc ttactttcgc cagcggactt agagctttta
    42601 tgcgtcaaga cccagatata ataatggttg gagagataag agatactgag acagcagaaa
    42661 tagcaattag agcttctctt acaggacatt tagttttaag tacattacat gcaaatacag
    42721 cagttggagc aatttcacgt cttttagata tggatgtgga aagtttttta ataacttcat
    42781 ctgttttagg tgtaatatct caaagactta cacgaaaaat atgtgagcat tgtaaggttt
    42841 catatgaagc agatattgga gaaaaaaagg ctcttggtat tgatgtaaat gaatcagtaa
    42901 ctatttatag aggaaaaggt tgtgaaaggt gtaataatac tggatataaa ggacgtctag
    42961 gtatatttga aatgcttgaa attactccag aaataaaaga gttaatagat tcatctgcaa
    43021 atcaaagaga aattttaaag atggctagaa aacaaggtat ggttagctta aaagaggata
    43081 tagttaaaaa ggttttaaat ggaaagacaa cagtagaaga aatgattaga ataattttga
    43141 tgactgatta aaggatgata ttatgaaaag ttttaaatac acagtaataa aagaggacgg
    43201 aaaaaagcaa actgatgtga ttgaagccaa cgattttaat gaagctagaa atcttcttag
    43261 aaagagaaat cttagagtta ttgaaatcaa agaaagcaaa aataaaaata taagattatc
    43321 tagtagaaag agaaaaaaag acttaggagc tgaccagata agccactttt gtagacaatt
    43381 tgctattata gtatcttctg gtataaatag tatttctgga ttagaaacat tagctagaag
    43441 gtcaactaac attacattaa gagaagaaat taatcgtata gtagcagaaa taaaagtagg
    43501 ttctactatt gcagattcaa tgctttcatc aaaatcaaag tttccaaagt tacttggtgc
    43561 tatggtagct acaggtgaag ctacaggtaa attagatgaa gtattaaaaa gtatggcttc
    43621 attttatgca agtgaacaca gggttaaaca aaagcttaga aatgctgcaa catatccatt
    43681 aatagttttg atttcttcat ttataatgat ttttatattt acaacattta tggtgcctaa
    43741 gatgattaat tcaattacaa cagtaggagc aactcttcca cttataacaa gaattgttat
    43801 ggggtttggt atgtttatga agaagttttg gataatagta cttttagtta ttatattatt
    43861 tatctatcag tttaaaaaat atttaaaaac gtcagttgga agggctcata aagatagagt
    43921 tataaacaag atacctgttt taggaaaagg aataaattgt atggtagcta cacgattttc
    43981 aagagcattg tatctatttg tatcaacagg atacccatta gttcaagggc ttgattatat
    44041 tatagatagt gtaaataaca ctatggcaga gaagatttta gcatcagcta aagatggaat
    44101 aacaagagga gaaggcttag cagaaaattt agagagatat acgtattttg actctgtgtt
    44161 ggtacagatg atagctatag gggagcagac aggggaatta gagaatataa gtagacagat
    44221 ggcagaattt tatgagtatg aatccgagat ttatttaaat aggatggcct caatgataga
    44281 gcctgtttta ataattgcag ttggtattat agttgcgata ttggttgtat caatatttat
    44341 gccaatgctt agtatatatg atgccatgta ggagggaaag gacaaatgaa caaggtttat
    44401 gtatcatatt atggcgattc tatttccata gttgagggca gatataaaca cgataaggat
    44461 aagttttatg taaaaaattt taatgtgttt tcaatagagg atattgcaca agatttttct
    44521 aaggaaaagt atgctctatt gagatacgca ttagaaaatg ttaagttaaa atcaagacat
    44581 gtagtttttt gtttaaatac aagagatgta attttaaaac cccaaaaact tcctaatatg
    44641 gacaaaaagg atttagacgg atttatgaag ctagagatgg acgaaatgat ggctttggag
    44701 tcagatgaat atgtattttc gtatgaagta tctagtgaaa cacctgataa ttcagatggc
    44761 gatgaatcaa gcttaaatct tattattgga gctatacaaa aggaagaggt aaatgcaatt
    44821 gtagagataa tgcatgaatt tgatttagta ttagatagaa tagatacatt atcaacagct
    44881 tacttaagaa tgcttaaaaa tttagactac aatgatataa tggtaaccaa tataagtgac
    44941 aatagcacaa ttataaatat atataagaaa gatactttat ttattagtga taatatacct
    45001 ataaagataa ctgctaaaga tatggacata caattattaa atgtagtaga agaatcaaaa
    45061 ggtcttatga attactattc gtcaagaaac tttggtaagg ttacggatac tatattaata
    45121 ttaggaaaaa gatattccaa taaattaatt tatgaaactt ttagctctgc attttcaagc
    45181 tatgtttctc aaggccttac tgagataatg gatatatctg atatgataac tggagatata
    45241 gatgaagatg atataaattc aattgtagag cttttaggaa gtatgataga acctaaaggg
    45301 aaaagagatt ttgaaaatat aaacttttta ccaatggata tacttagaaa acagataaaa
    45361 gctaaacaaa tgaaaaacat actgaaaatt gctccaatag ttattttgat tcttgctata
    45421 ccttatttat gtttaatttt ttcaaataat ttagcaaata aggaacttga aaatgttaat
    45481 ggtgaaataa gaaatattga atctgcatat tatcagattg gaaatataga aaaaatgata
    45541 cagagtaaaa gcgaagaaat aaaggtatat gatatgttaa ttgggaaaaa ggtaaagtgg
    45601 gaaaatttat tggatgatat agataaaaat actccaagta aagttgaact aacaagttta
    45661 tcaacaactt atcaaaatct tggaactaat aataaagctg ataattcaaa aagtaataag
    45721 actacaacag ataaatcaag taacaatgaa acaaaatcaa gtacgaaaag tgatagtaat
    45781 ggagataaaa cagataatga tttaaatgaa gataagacta caaatgtgtc agaagcatta
    45841 actggaaata gtgagactaa tagtaataac acagataaag atgataaaac tccgttgtat
    45901 gataagatac caaatgttat aaatatagaa ggaaatgcta aagattcctc ttatgtaggt
    45961 caatttttat ataaattaaa gggattatac tatttcaagg atgttaaatt acatagcata
    46021 aaaagtaatg cagaagattc taactataca tttagtatga cattatacct gaaagaaggt
    46081 gtattaacta atgaataaag atatatttaa aaaagatata aaagatatat ttaaaatgga
    46141 agttagtcta aaagggattc ttataaaggt tataatttta ttagctattt tatcatatgg
    46201 ttattttgga ctttaccctc aatatcagaa atatattgct acaaaaaatt ctgtaaaaca
    46261 ggctactgaa aaattaaata tatataaaga gaaaactgca cttatgccaa aatatgaaaa
    46321 acaacttgaa gaagttgaaa gtgaattaaa agaaaaaagc aaaaatcttt tatatgatat
    46381 gcaagatggt ctttttttaa taggtttaga taaaaaaatg aaggcttata atattgactt
    46441 aaccagttat gacgttggaa atgttaaaga atacgaacat ttttatggta tatcaacaac
    46501 aataagtgtt agaggtgatt atagaaatgt caaaaaatta atggcttatt tagaagaaca
    46561 aaaaaatgtt actcaaatac tagattatac aatgacttca tatatagaac cagaaaaaga
    46621 agaggaagaa acaacaaata ataaggattt aattaaaaat gtgaacataa ataaagatac
    46681 aaaaatatac tgggtggaag gcgaaaataa gtggcattta tataaggatt gtccattgat
    46741 aaaagaggga gagattctaa aaaatgacaa acttttagat aggtctacta attatctatg
    46801 taatacttgt gtacttagag ctgcaacaga aaaagaagca aaaaaagaag tcgtagctcc
    46861 aaaggcagaa ggtgtagtag atgctacatt taaatttata atgtacacta aagagaatac
    46921 tacaatgata cttgatacag ataatccatc ttcatggaga ccaggaaaat ataatccatt
    46981 taagtctagt gcgaaataaa gctaagtaag ctttaaagat aggtgatgag tttgtacaaa
    47041 aataatgaaa aaggtcttac tttattagaa gtaataatag ctgtttttat actgacaata
    47101 gttttaagta tttcttataa agtgttcaat ggtataacat cagcagtaaa aaagcaacag
    47161 attattacag atgctcaagt aaatattaat ctaattaata agtatctaaa tagagatttg
    47221 gaaaactgta aagaactaac taaaactggt tcaggtaata attatgaata caatatagag
    47281 atgccagata atgttgtaaa atatgaagtt tctatagaaa ctaaaaaaaa tacagaggta
    47341 tattctgtaa cgagaatcca aaacaataca attgatacag aaaatgaagt tagagaagag
    47401 ataatctaca atcaaccact agttcaaaac aataaagaaa tgaaggaaac accatttaaa
    47461 atagaaaagc aaactggtaa atctatttat actgtaagta tatactataa tgaatcagtg
    47521 caagaaagtc ataaaaatag caatttaaat aacaaaactt atacatttga tgttatgtca
    47581 agaatagggt aataagaatt tattaaaaaa taagtaggtt gataaaatat gagtaaaaag
    47641 aatagtgaaa gaggattttc cttaattgaa gtgttagtag ctatggctat tatgggaatt
    47701 gtactatttg cattttttaa tattatcaat actaataaca aagcaaacac taaaaatgac
    47761 acagatataa cttccttaaa ctatgtccaa agtgaaatag aaaatctaag agaaaagata
    47821 aaaagtggag aatttgattt tgatagttta gataaactag aagatggaac tgtcgtatat
    47881 gaaaaattaa tagataaatc aaaaaaagtt gtatatgata aagttcttag tgaaggtgat
    47941 gtgagcttat atgatactcc atatgaaaag attacaacaa taaaagatga agatggtaat
    48001 ttaatcgaca aaggaaatat tacaaataag ataaagacta ttgtagaaga taaaagtggt
    48061 cagatatata aaatagctgt aactgggaaa agcatgaatg actattcaag taaaaaagag
    48121 gttaaaattg taactgaaat atttaaagat aaataatttt acttgggggt gagattttga
    48181 ggaagtggaa taaatttaaa agtgaaagag gggcagcttt agttttagtt ttggttgtag
    48241 tagcattgct ttctattgtt ggattgatat tttcaaatca gatagctaat agaataaaat
    48301 ctacaaagac tactaatgaa ggtatacaag caaagtattt agcagagaca tgtgtggaaa
    48361 atagtattga taaagcatat gaaaaattgt atgatgagct tgagaaaatg gataatgagt
    48421 ttaaaagtga aaatcaagaa aaatccatat ctaggtcaaa attaagaaat atatcagatg
    48481 aagattttaa taatcaagat gaggaaaata ttgaagcaga aagacttggg tatatgaata
    48541 atataaattt ctatttaaat aaagcttctt cagacttgga aaaagctagt atggaattga
    48601 aaaagctata tgatttagat atgttggact atagagacat tgaatatgta gatgcaaata
    48661 ttatttcaca tagagattct atcttagaaa tttgcaagaa ttatacttct ggagatatta
    48721 gtaaaataaa tgaatatatt ctgaaagaag atatagactc aactacattg attgaggcaa
    48781 agttagtgaa taatgatatt cttttgaaaa tgtttttaga agaaaataaa attgaaaatg
    48841 aacatttaaa ttcagccttt tcacatactt ataaggcatt agataacatt tctttggcga
    48901 tgcaaaatat gattgagtat agacatacgt ttcatataga tgagccaaaa gtagaagtat
    48961 ctaatgggat tccagactct caacaatatt atgaactaat acaaaatcct ataataaata
    49021 gtatggagta tagctggaat agtaaatggg atacattaga aaacctattg gaaattttac
    49081 cagatcaaac tcaaggattt aattctttaa gagttcattt gagaaataat gtaagaaaat
    49141 ttgagaagtt aagcgataat atatctagtg gtaaaaaaaa tacagcaaaa aatttcttaa
    49201 agtataaaga gttattgtat gaaatatcag accaatgtaa tcaactaaaa agtatgtctt
    49261 atgagaaaat acctgttaaa tatgataaca tggctttgat aacaactttt gactatatac
    49321 aaaatgagct tttagcagag ataaaatgca gattaaaaga attaaaacca caagaaattg
    49381 ataaaacaga aggtattact ataaaaatac ctttttataa ggctgactac gacatgacaa
    49441 aagaaggatg gcctaaatta aaagaaaatg gttcaggggc ggagttaagc ttaatggtaa
    49501 ctggtgataa agatggaata aaagaagtag aagttacaga tggtaaaaaa aatattattg
    49561 gtttaggtgt tgaagaaaat tcaaattcaa agtataaggt tgacgccatt gtaaacttta
    49621 atctaaatat agatacaaat gtagttggaa attatgatat aaaagataaa attttgatta
    49681 atcatgatat aagttcatac aaaaaagtaa actaaatata agaagcaact tagtataaat
    49741 aaataataaa ataattttat tatttactac taaaaaaata agtaaactaa tagaagatat
    49801 ggagagtaaa tattatgtat atttatgatt tattagagca aggtataaga ctaaacgcat
    49861 cagatataca cataaccgtt ggaacaaatc ctgtagctag agtaaaaggt ggatttgtaa
    49921 aactaagtga acaaatattg acatcagaag taacaatgca aatggcaaaa gacatagcgg
    49981 gggaatctat gtttaaagta atagaagaac atggagaagc tgatttttca gcttctctaa
    50041 aaacaggaga aagatttagg gtaaatgcat ataggcaaaa aggaaattac gcaatagcaa
    50101 taaggactat aacagcagaa attccaacat ttgaaaaatt aggccttcca gagagtataa
    50161 aatcttttac tgaaaagcat aaaggattag ttttagttac aggtccaaca ggaagtggta
    50221 aaagtacaac acttgcaagt atgataaata taataaatga aaaacaacaa aaacatataa
    50281 ttacacttga agacccgata gaatatgtac atcatcataa acaaagttta gtaaatcaaa
    50341 gagaagtagg aactgataca gaaagttttc attcagccct tagagctatt ttgcgtcagg
    50401 acccagatgt aatactaata ggagagatga gagaccctga aactgtttca atagcattaa
    50461 cagcagcaga aacaggtcat ttagtatttt caacactaca tacagtgggg gcagctaaga
    50521 caattgatag aatagtagac atgtttcaac catcccaaca gcaacaaata aaaacacagt
    50581 tatctacagt ttgtgaaggt gtggtatcac agcagttact tcctacagca gatgggaaag
    50641 gcagaatagc agcaattgaa ttgatgtttg ctactccagc aataaaaaac ctgataaggg
    50701 aaggcaaaac atatcaaatt ccaaacatga tacaaactgg agttaaagta ggtatgaaaa
    50761 ctatggacca agatttaatg gaactttata aaaatggaaa gattacaaaa gatatggcac
    50821 ttagtaggtg tactgaccaa gagtttatga caagaatgat aggtggagta aactacaatg
    50881 gatattataa tcgttaatct atatgtattt atagtgggta tagtgtttgg aagtttcttt
    50941 aacgtctgta tttttaggat tccagaaggt atatctattt ctagacctcc ttctcattgt
    51001 atgagttgta atactagact aaagccaata gacttaatac cagtgttaag ctatttaatg
    51061 agtggcagaa agtgtagata ttgttatgag aaaatttcta gtagatatgc tatagtggaa
    51121 ttaattacag gacttttatt tttaacagta tttatgttat atggaatatc tacatcaact
    51181 atttattatt tggttcttgt gtctttactg attataatta catttataga tattgaccac
    51241 tttataatac cagataaaat attggttttt ggagctatat tttctatgat ttttaacctt
    51301 ttatttaaag aaattccagt taaggattca attctaggtg tttttatttg tggaggaagt
    51361 gtctggatta tagttctttt gattgaattt gtaataaaaa aagagtgtat gggtggagga
    51421 gatatcaaac tatttgcaat gctaggtttt tacatgggtg ttaaaaatgg tcttttgaca
    51481 gccttactaa gcgtttatgt gggagcagtc tatggaatct gtgtaataat tataagtaga
    51541 ataaaaagaa aggaatataa ttctgttata ccatatggac cttttatatc tattggagca
    51601 ttaataacaa tcttatgtgg aaagcaatta cttgaacttt atttttctat ggttatatag
    51661 atgatagtca agttaaataa agttttaatt taaaaaaatg aattaaagta tttaataagg
    51721 acaatattat tattaaccct attactaaag tgctacaaac tatggagtat ataataataa
    51781 tttccttatt ttttgcacat ttgataaata aatctctatg tgttttttgt gatagagaaa
    51841 agaaaaattt atacaatacg ttttttattt acataataac tgtagtttta tctgtattaa
    51901 tatatctaaa atttgggatt agtgtagaat gtgtacaata tatggtttta attccatttt
    51961 taataattat atccattgta gattactaca caatatatgt atatgatata acaatagtta
    52021 gtggtataat agttcaaggt ataatttttt tggtcttttt caatatagtt caatttgatt
    52081 atataaatca tatatttgga ctattgatag gctttattat accttatatt attgctaaac
    52141 taacaaaagg attaggctat ggagatgtag gtctctatag cttatgttgc tttgcattgg
    52201 gaaataatta tgggttttat gtgatagttt tatcatttat tttagcgttt ttgtttttta
    52261 ttattttata cataactaaa atatttaaag atgtagatat aagaaagcaa attcctttta
    52321 caccttttat ttccttgtca acaattttaa taatatttac agagtataat atatttaatt
    52381 tttattataa tttgttatac aaatttatat aacaggaggt aagaaatgta cgtagtagta
    52441 gggttaggta atccaggaaa gaagtatgag aagactagac ataacgttgg atttgatgtt
    52501 atagatattc ttgctaaaga atacaatatt agtgttacta aaataaagca taaagctttg
    52561 attggtgaag ggagagtagg tactgaaaaa gtattattag ttaaaccaca aacttatatg
    52621 aatttaagtg gagaaacatt aatagatata tataaatatt ataaggttga tttaagtaat
    52681 atagtagtag tatatgatga tatagattta gaagttggaa agatacgtat aagaaaaaaa
    52741 ggaagtggag gaactcataa tggcatgaaa tctataacta agtgcctagg ttcaaatgac
    52801 tttccaagag tgagagtagg tgtatctaaa ccagaagctg gtcaagactt agcagacttt
    52861 gtactttcta gatttagaaa agaagaatct gataacataa atgaagcatt agaaaaagcg
    52921 gcagatgcta ttgactcaat aataagagaa aatatagata tgtctatgaa caaatataat
    52981 ggatagtgag agggagattt aaatgaatga tgttttttta tatcctttgc aaaattcaaa
    53041 agagtataaa gatataataa actgtataaa aaatacaaaa ggctcattat tggttaatgg
    53101 acttttacca gtacaaaaac ctcatatatc gtattctata tttaatgatt tatcacgaca
    53161 aatgattttt ataacaagta gtgatttaga agcaaagaaa gtatacgaag atttaagttt
    53221 ttatatggaa gataaagttg aatatttagg ttttcaagat atttattttt atcacttgga
    53281 tgcaaaggac agaaacgaag aagcgaagaa attaaaagta ttgttaaaac tagcaaataa
    53341 agaaaaaatc attttagtaa cttctattga ggcagtttta agaaaataca taccaaaaca
    53401 agttttgctc gatagtgtat ctcattataa ggtgggagat agcttagact tagaaaagtt
    53461 aactgagaaa ttagtttcat tgggatatga aagagtttca aaaatagagg ggtttggtca
    53521 atttagtata agaggtggaa ttattgatgt attttcactt gaatatacaa atccaattcg
    53581 tatggaatta tttgatgatg agatagattc catacgaact tttgacgtat attcacaaaa
    53641 atcaattgat aaattgcaac aattctctat aactccatct agggaattta tttatcccga
    53701 aaaaactaca gatgcattag ttaaattaaa aaaagaaaca actaaaaata ctgatgaagg
    53761 tacatttcaa aatatagatt atatatcaag taagacttat tttgaaggtg tagagaacta
    53821 tatagattat atttatccag aagaaaacaa aagtatattt acttatttag cagatgatgc
    53881 aatagtattt attaatgata taacgagact taaagaaaga tgcgaaaatt atataaatga
    53941 gtttagagaa aattataaat taaatcttga aagaggtctg gctattaaaa accaaggtaa
    54001 attgttatat cactatacag atttagagta cttagtaaaa gacaagtctg tggtattaaa
    54061 ctcgctattg ccaaagtcta taaataactt ttctataaaa tctataataa attttgaaag
    54121 tagagaagtt cctacattta atggaaaggt agatttgctt gtagaagaat taaatcggct
    54181 gaaatataat ggatacaaaa taattttagc aacaaatacc ttagaaagag caaataaatt
    54241 aggaaaagat ttattggaca aaggtttaga aacgaccatt tctaaagata gagatattga
    54301 aattaaatct tcacaagtta taattgtacc tgcacacata aacagtggtt ttcagtataa
    54361 atctataaag tttgttgtta taacagataa cgaaatgata ggtgtttata aaagagcgtc
    54421 taagactagc aataaaaaag ttaaaaaggg aaagaaaata gaatcctttt tagatttaag
    54481 tgttggagat tatgtagtcc atgaaaatag tggtgtaggt agatacacag gaattgaaca
    54541 aattacagtc aatgctatta aaaaagatta tatgaaaata gtatatcaag gtggagataa
    54601 tctatatgtg ccaatagacc agatggacaa agtacaaaaa tatattggtg cagaagtaga
    54661 aaaagttaag ttaaataagc ttggaacaaa tgagtggacg aaagcaaaag caaaagttaa
    54721 aaaagaaatt gaagatatga caaaagactt gattgagctt tatgcaaaac gtgaaaaaat
    54781 acaagggtat aagttttcaa aagatacacc ttggcaggca gagtttgaat cgttgtttcc
    54841 atatcaagaa acagaagacc aattaaaggc tatagaagaa actaaaaaag atatggaatc
    54901 aagtaaggta atggataggc tagtatgtgg agatgttggt tatggaaaaa cagaggtagc
    54961 tattagaagt atttttaaag cttgtatgga ccaaaaacaa gttgctgtat tggttccaac
    55021 aacaatattg gcccaacaac attataatac attcaaagag aggtttgaaa attatccttt
    55081 gagagtagaa gttttaagta ggtttaaaac accaaaacaa caaaaacaga ttattgaaga
    55141 tgctaaaaaa ggattagtag atattttaat tggaacacat agaataatat ccaaagatat
    55201 aaatttacca aacttaggtc ttgtagtcat tgatgaagaa caaagatttg gagtaaaaca
    55261 taaagaagct ttaaagaaaa ttaaatctac agtagatgta cttacacttt cagcaactcc
    55321 tataccaaga acattacata tgtctttaag tggaataaga gacatgagtg ttatagaaga
    55381 acctccacaa gaaagacatc ctgtaataac atatgttaca gagagtaagg aaagcgtaat
    55441 tcaagatgag atagagagag aattaagtag aggtggacag gtattttttg tatacaatag
    55501 agttgaacac attgaagaaa tggcaagtat gattcaaaaa ctcgtaccag atgctagagt
    55561 ggctgttgca catggaagaa tgacttctaa atcattagaa aatataatcc taggattttt
    55621 aaataaagat tatgatgtac tagtctgtac tacaataatt gaaactggta tggacatatc
    55681 aaatgcaaat acaatgatta tatatgatgc agataaaatg ggattggctc aactttacca
    55741 attgagaggt agggttggaa gaagttcaag acaaggatat gcatatcttt tgtatgaaaa
    55801 agataaaact ttaagtgaga tagcggagaa gagattaaaa gctatcagag aatttacaga
    55861 gtttggttct ggttttaaaa tagcaatgag agaccttgaa ataagaggag caggaaatat
    55921 tttaggttct caacaacatg gtcatatggc agttataggt tatgatttgt acgttaaaat
    55981 gcttaatgat gcaattaaaa aagtcaaagg tgaacctatt gtggaggaaa ttgatgttga
    56041 aatagattta agtgtaaatg catacattcc agataattat atcaaagatg agttgattaa
    56101 aattgaaatg tataagaaaa tagcttctat tgaaaacaaa gaagacatgt tggatataca
    56161 agaagaactt gaggatagat tctctgatat acctaaacca gtccagacac ttcttacaat
    56221 agcttatata aaatcgttat gtaagatatt aaaaatagaa aagataagac aattaaaaga
    56281 tgaaatatta ttagttccaa taacaaagta tagaactaaa cagaaaatag gttataatat
    56341 agttacagaa ttagaagaat tattagaaaa gatgtgcaag gtaaaataac ttgtaaattt
    56401 aagaagggat gttttaaatt tgaaaaaagt aataacattg gtaatagcta tgattttggt
    56461 agtatctgtg actgcttgca gttctagtaa aggagaaact gtggcaacag ttgaaggtac
    56521 aaagatatca tcagatgaat ttaaaaaaac tatagctctt tataaagatt ctatggaaca
    56581 aacttatgga aaagatatat gggataagga agttgaaaag ggtgtaaaat acaaggacaa
    56641 gtttaaagat ttaattcttg accaacttat aactacagaa gtaatatatt ctcaagctaa
    56701 aaaagataac ttacttccta aaaaagagga tgttgaaaaa agctttaaag aattaaaaga
    56761 cgctatgggt aaagatgaaa aatataaaga acaacttaag aaattaggta tagatgatga
    56821 gtttttaaga gaccaacaag agaaagattt agcaatgcaa aactatcaaa gtaactttgc
    56881 taaaaagact aaaatatcag atgaagaaat gaaaaaatac tatgatacac ataaagatga
    56941 atttaaaaaa gatgaggtag aagcttctca tattttattg aaaacagtag atgataataa
    57001 caagccactt tcagataagg aaaaagctga agctaagaaa aaggctgaag aggctcttaa
    57061 agaagttaaa tcaggtgagg attttgcaaa agtagcaaaa aaatattcac aagatacttc
    57121 agcaagtgat ggtggtaaat taggattctt ttcaaggggt caaatggttg ctgaatttga
    57181 agatgctgct ttttctatga aaaagggtga agtatctgat ttagttgaaa ctcaatatgg
    57241 ataccacata attaaagtga cagacagaat aaatgagcaa acatcatttg aagatgctaa
    57301 agaaactata aaagaccaac tgttgaaaaa taaatatcaa gaacaaatag aaaaattaac
    57361 taaagaagct aaggtagaaa aagatgaaaa agtaataaat aaaatcacaa tctaagactt
    57421 aaaaaaagag taatatgctt aaaaaagcta tatctggata agatatagct ttttttgtat
    57481 atttttctta acaatggtaa aaataaactt aaagtaattt atggagggat taaaaaatat
    57541 gaaagcaaca ggtatagtta gaagaataga tgatcttgga agggtagtta tcccaaaaga
    57601 gataagaaag acattaagaa ttagagaagg agacccttta gagatattca cagcaaaaga
    57661 tggagaagtc atacttaaaa aatactctcc aataggtgaa ttaaatgaat tttcgcaaga
    57721 atatgctgaa acattagctg aagtactagg atatggagtg ttgataacag acttagattc
    57781 tataatagct gtgtctaagc ttcctaaaaa agattataaa gaaaaaagta taagtgatga
    57841 gttagaagca ataataagtg aaagaacagt aaaatattca aaagataaaa acattttacc
    57901 attattcaag gaagaccaaa aagaatatac tagtcaagtt atcatgccta taattagtgc
    57961 atctggtgac tgtataggtt caattgtagt tgtttcaaag gataaagata caattgcaga
    58021 aagtgaagaa aaatctttaa aaattgctgc taacttttta ggaaaacaag ttcaataatt
    58081 ttatcataaa gaccttttac atgaaggtct tttgaagttt ttgtgtaaaa tattgaatat
    58141 agcattaaaa atatggtata ttaaagtttg ttatataaac agtatggagg ttatgttatg
    58201 aacaataaca aaaataaaga ctcctttcta aaaggagccc ttatacttgg gctagcaggt
    58261 gtaatcgtaa aaataatggg tggatttttt aggattcccc ttggaaacat gattggttca
    58321 gaaggaatgg gatactatca ggctgcttat cctgtgtata ctctattttt aacattagct
    58381 actgctggat ttccaacagc tgtggcaaaa ttggtttcgg aaaaagttgc aattggaaat
    58441 tttaaaggtg caaataagat atttaaggta tcacatacag ttttattcat taccggtata
    58501 atatcatttt gtatattatt ttttggagcc gactacatag taacaaacgt aatgaaaaat
    58561 ccaggtgcgt tatactctat gaaagccatt gctccagcat tactatttgt tcctgcaatg
    58621 tctgcatata gaggatattt tcaagggaga caagatatga ctaagattgc tgtgtctcaa
    58681 gttgcagagc aattttttag agttgtatta ggtcttactt tggcttactt gcttatgaag
    58741 agtctaggtc agacttttgg tgcagcaggt gctatatcag gggctacaat aggttcaata
    58801 gcatctatgc tttatttggt gtttgcttat atgctaggga gaaaagagcg tagagcagag
    58861 atagatgcaa gccaaagatt taaagatgaa agagtgtctt atatttttaa aaagctttta
    58921 actgttgcca ttcctataac aataggagca tcagtaatgc cacttgtaaa tatgatagat
    58981 aatgttattg taataagaag acttatggaa gcaggattta catacaaagt ggcaaattca
    59041 atgtttggtc aattaacagg tatggcaatg gccacaataa atttaccagc agtcataaca
    59101 actgcaatga gtatgagctt agttccagca atttctaaag catatgcatt aggaaataag
    59161 tcaaaagcta gaaaagatac taagagtgca gttaaggtaa ctttacttat agtattgcct
    59221 tgtgcgtttg gtatggcttc tttagcaata ccaataatgg gtcttttatt tccacatgaa
    59281 ccatcttctg taggaactat tttgtttact cttactccat gtgtattatt tttaggatta
    59341 atacagacat tgactggtat aattcaggga atgggaaaac caatagtacc tgtaattgct
    59401 ttatgtgttg gtatgctatg taaaattgta ataagttata ctttaacagg tataccagat
    59461 attaatgtac tgggttcagc atttggtaca gttacagcat actttgtggc agccatgatt
    59521 aacttactat atgttaaaaa acacatgaat gtaaactttt ctaaaaaaga atttataata
    59581 aagccattta taacagttat gacaatgttt ataatggtta agttgagtta tggtgcttta
    59641 gttggattct taggaaacag catatctaca ataattgcaa tatgtgttgg tggaattgta
    59701 tatgtagtag taatacttgg aataggtgga attaaaaaag aagagattct aactatgcca
    59761 aaaggtgata aattatataa attgttaaaa aaagttaaac ttataagata atataggaat
    59821 atatatgcaa ataaaggaaa tactatacaa atagtaaatc tccttatttg tataataata
    59881 taaaaataaa gtgaaatttg ctaataggag tgatgttatg ggtaagataa gtattgtagg
    59941 tcttggacca ggagactatt ctcttataag ccaaggtgtg ttagaatctt taataaatag
    60001 ccctaaagtt ttttttagaa cagagaaaca tccaataatt gataaactta aagaaactat
    60061 acaatacact tctttagact acttttatga aaaagaggaa aattttgata atgtttattt
    60121 gaaaatatct gaatttatca tagaagaatc agcaaagggt gacctagtgt atgctgttcc
    60181 tggtcatcca agagttgcag agaagactgt aagtataata gagagattag ccaatgaaaa
    60241 gcacatagca gtagaaatat tgccttctat gagctttgta gacgctatgt ttaactattt
    60301 ggctatagac ccatctgagg gatttaaact ggtagatgct tttgaaatag aaaattcata
    60361 tatagacttg gatacaagca tgataataac tcaggtatat gataaattta tagcatctaa
    60421 tgtaaagtta aaacttatgg aacattatga ctataatcaa gaagtatgta tagtaaatgg
    60481 agcaggtgtc aaagatttag aaagtaaaaa gtttataaag ctttgtgaat tagacagaaa
    60541 tgaaaacttt tttgactatt taactagctt atatataccc aaaagttcta aaaagatgta
    60601 taatacagta catgacttag aaataatagt taatacactt agaagtccat ctggatgtga
    60661 atgggataaa aaacaaacac atcaatcatt aaaaaattct gttatagagg aagcatatga
    60721 actttgtaat gcaattgata ataatgatat agatgaaatg gtagaagagt taggtgatgt
    60781 tcttttacag gttatattcc attgtcagat aggaaatgag gagggctact ttgatttaaa
    60841 agaagtagta aatggcatat gtaaaaaatt aattcataga catcctcata tatttagtgg
    60901 tgcaaaatta gatatgtcta agtttgagaa aacttgggaa gaattaaaga aagaagagaa
    60961 aggcgaaagt actataacag atggccttag aaggatacca aaacatttac cagctcttat
    61021 gaaggctagt aaagttcaac ataaagcagc acttgtcggc tttgattggg acaatgtaga
    61081 agatgtattt aaaaaaattg aagaagaata tcaggaatta cttgacgaac acagggtcgg
    61141 gaatataaaa tacataaagg aagagctagg cgatttatta ttttcgatag taaatctagc
    61201 tagattttta gacatagact caggagaagc cttgaactgt actacagaaa aattcataaa
    61261 tagatttgac tttatagaaa aaagtgcaat gaacttgaat aaaaaattag aagacatgac
    61321 acttgaagag atggatgagt tttggaatca ggctaaaaaa caataaaatt gtggaaggaa
    61381 atttgtcaaa aatctagaac ttttatcaca aagatagaag tctatattat aaacattttt
    61441 aggaggtaaa tatcgtgaat aaagctgaat tagtatcaaa gatggcagaa aaaagtggat
    61501 taacaaagaa ggaagcagaa gctgcgttaa acgcatttat gagttctgtt caagatgcac
    61561 tagtaaataa tgaaaaagtt caattagttg gatttggaac atttgagaca agagaaagag
    61621 ctgctagaca aggaagaaat ccaagagatc cagagcaagt tatagatata ccagcttcta
    61681 aagcaccagt tttcaaagct ggaaaaggat taaaggatat aataaatgga taattagaaa
    61741 tcttgtgaaa aagtgtggat aaatttgcca cactttttct atagaaccaa ggaaggtgat
    61801 aattatgaga ttagataaat ttttaaaagt atctaggatt ataaaaagaa gaactgttgc
    61861 taaagatgca tgtgataaag ggattgtcac tataaatgga aaagttgcta aatcttcttc
    61921 agaggtgaat attggagata cattagaaat acaatttgga gagaagaaga tgaagtttaa
    61981 gataaatgaa gtaaaagagc atgtattaaa aaatgatgca aaagaaatgt acgaaatttt
    62041 agaataaaat taatagtcct ctagtatatt aatagtagta ttattgaaat atttactggg
    62101 gggatattat ggaacagaac ataactttga aagatagatc aaagctggtt atttcgggag
    62161 tcgagcacat atactctttt aatgacaaaa gagtagagct caaaacttct gtaggcgaga
    62221 tggtaattga aggcgaaaac ctagatatga gcaagctaag tatagacgaa aatataatta
    62281 gcatagatgg aactataaat tctatggtat atgcaaagcc taaaaagcca caagagagtt
    62341 tcttgaaaaa ggtatttaga tagatgatac cttttactca agatgtaagc attttttatg
    62401 ctactattta tggtggaata ctcatagggg tgttgtttga tttttataga gggctaagag
    62461 ggaattttaa attcatcaat tattttgcta taatctttga tgtattgttt tggtttctag
    62521 ctacggttat aatattcgta acaataaacc ttacagaatt ttttgattta agatattatc
    62581 attttgtggc tttgtttatt ggttttattt tatactataa caccataagt aaaattgttt
    62641 tatcaattat caataaaata attcgttttg tgagaaattc ttttaaaaaa gttacacact
    62701 acatagtcag ttttttaaat aatttgtatt acgttataat ctattcacta cacctactat
    62761 ttgatattat attttatata ccaaacatat ttatagcaac aagaaaaagt ataaaaagaa
    62821 aatcaaataa aaaattaaag aacaagaaaa aatcaaaacc taagaagaaa aaaaataaaa
    62881 ctaaaaaaag ggtgtaggaa aaatgaactt aagaaaaaaa ttttcaggac agattattgt
    62941 tatcagttta tttttaggaa tatcaatttt tagtatgatg acaggatttg tatttgagta
    63001 tacaaaagct aaggaatata aaaaagaaat agcaagtcta aataaacaat taaaaaagac
    63061 tgaaatacaa ataaacagct taaagaaaga tgaaaaatca tatgaaggag acttagagga
    63121 tatagcaaga aaaagattga atatggtaaa accaaatgaa acagtttatg tagacataaa
    63181 tcgttgatta aaacttgcta ataagtttac agatgtattg attttaaaat ttaagattta
    63241 ataaaaaagt cattcagtta aggatggctt tttgttttta ttgaggaggg aatttcataa
    63301 tgaaagtagg agctatagat attgggacaa actcaatgag attattgata gcagattata
    63361 gagatgggaa ttttataaac agaaaaaaat ttgttaatac aactagaatt ggttatggtg
    63421 tggatagtaa aggatatata aaagaagatg ctatcaaaag aaatataaaa gcattaaaag
    63481 aattttctga taagtgtaat gatgaaaagt gtgaaaaaat ttattgtatg gggacatctg
    63541 ctcttagaga cagtaaaaat ggagattact ttgttaaatt agctaaagaa gaaactggaa
    63601 tagatgttga tatagttacg ggaatagaag aatcaaattt gggctttaag ggtgttttag
    63661 aaggaataca aggtgatgat actattattg ttatagatat tggaggaggt tcaacagaat
    63721 ttattgtagg tgattcagaa ggaattatat ttaataaaag tgagaatgta ggtgctctta
    63781 ggatgacaga gaggtttttg aagcacgata ttattgaaga tgaagagttt gaagctatga
    63841 gttcatttat atatggtgag attgataaaa cgttaaatca tataaaagac agaggattaa
    63901 aaactcttat aggtatagga ggtacgatta catcattatc agcaatgaat caagaattag
    63961 agatttactc tatggaaaaa atacataata gtaaaataca caaaaaagaa atagaagaaa
    64021 ttctacaaaa attaataaag atgacattaa gtgataaaaa gaacttaaaa gggctacaag
    64081 aaaaacgtgc ggatattata actgctggtg taaaaatatt gaatataata atggaaaaat
    64141 tagaaataga agaaataacc gtaagtgaat acgataattt ggaaggctta atatgtcaaa
    64201 agtcaaaaaa aatgtattaa tattttttga tttttaaaac cgattatgac aaaaaaaatt
    64261 actcctcttt gttacaattc ttaatataaa caaaagagga ggttttttta tgcaaagaag
    64321 tgtagcagtt gtgaagaata agactgtaaa accaaatgtc ataaaattaa taagcggata
    64381 tattgatttt actacggcgg cattcgctgt gatgggattt ttacttagta ggtctattat
    64441 agtagactct gtagctcctt taggaattgc cttttttata tgtgtatcaa agattgataa
    64501 gtacaagata cctgtattca tatcaacatt attaggaata ttgttatcat ctaatgattt
    64561 tggaaatgta gcaaaatatg ctgtgtgcct tacaatattt atggctatga ataaaaaatt
    64621 aaaattatca gattcaattg ctaaggttgc atttataggt gcaataatac tacttccaat
    64681 ctccatggga caagctttct tttcaaatag gtatgtatat ggtcttctta tagcaggagt
    64741 agaagcaata ataatgttta tatctatata tgtgttttct tatgggatag gtttaatggc
    64801 caatgtcaac aataggatgt ctgtacggac agaagaagcc atatcaataa gtttactgct
    64861 aacctttagc ataatgggaa taggagatat atctctgttt ggaatatcaa taaggaccat
    64921 tttatcgacc gtattgatat tgctgacctc aatagtaggt ggagctacca tgggagcatc
    64981 tagtggtgta attattggtg tagcatccat tttaaataat atgacatcag ctgtttatat
    65041 ggggatatat tctttttcag gattaataag tggagcattt aataaaataa ataaatattt
    65101 ctgtatacta ggatatattc taagttggac aataatatac ctatatactt ctggtattac
    65161 ttctaatatg atgcaattaa gagatatact attaggttgt ttgatagtac tagtattgcc
    65221 agagcgatta tttaataaga ttgaaaaatt aataaaaagt aatgtggctt caaatgaaat
    65281 cgtatacgat tatattatga gaagcaaaaa cctaactaat agcagattga acagtattta
    65341 taaaacttat gatgaccttg cagatacatt tgataagata agagaaaaag acaaagtgct
    65401 agaccaaaga gacatagcta atgtaataga tatgattcat aatgatgaat gtaaaagttg
    65461 tagtatgaga agaatgtgct gggagtcaag atttaatcat acatacacta tgatatataa
    65521 cattcttgaa aaaattgagg agaaagggga gttaagtcta aacgatatac ctaaaaattt
    65581 tagaaaggaa tgtatgaaac cagagtcaat agtgaaaata tcaaatcact attacaaaat
    65641 gtttgtatta gattatgatt ggagtgtgaa gttttcagaa agtagaaagt taatagcaaa
    65701 tcagataaga agtatttcaa aatccattaa atccttatct caagatttag aaggtgacat
    65761 aatgctagat atagaaaagg aaaaaaatat atatgaacag ttagaaagat atgatataac
    65821 agttgataaa gtaagttact taacaaaaag taacagtgag tttgaaatat caatagaaaa
    65881 gaaaacttgc catgatggat gtatgtgtga agataaaatt gtaaatataa tttcagacct
    65941 agtaggtgaa aatatgtctg ttagaaaaat aggatgtcac tgtttaggag gtaaatgcaa
    66001 ggcaacattc gttaaatctc aaaaatataa ggctgttaca gaagtttcag caatgtcaag
    66061 ggatgggcat atcctttgtg gagacaatta cacatacatg gagataaatg atggtaaata
    66121 catgatggct ataagtgatg gtatgggaaa aggtaaaaaa gcatatgaag aatcttctgc
    66181 cactattgat atactggaaa aaatgataga tgcaaaaatt aaagatgaga tagtcattga
    66241 tactataaat aatatgttgt tgttaaaatc ttcagaagag atgttttcaa ctctggattt
    66301 gggaatttta gatttaaaac gaggttgttt agaaactata aagatgggag cgtgttctac
    66361 ttatataaaa agagaggatg gagaagttga cttaatatca tcatcatcat tacctgtggg
    66421 tatattatca gatgtaaaaa ttgatagaaa aaatgtaaaa gttaaggaag gagactatgt
    66481 tataatggtt tctgatggaa ttgtagatgc aggaagaaat aacaatttag gagacaattg
    66541 gttaatatac ttcttaaaga atatagagac tacaaatcca aaggaaattt caaatttaat
    66601 attggataga gcattagagt tacaagcgtt acaaatcgaa gatgacatga ctgttttagt
    66661 tacgaaaata tgtacaaaat aaggtttaca agattatgtg tataagatgt ggataacttg
    66721 tgtataatat gttaatagac ttttgaaaaa agtatagtta ttaacaaaaa taacatataa
    66781 atcaacaaaa tttgttgata agtatggcag aaatgttaat aaaatgtaaa aaaagaaaaa
    66841 acaaaagcta ttatcaaaag agattcatgt tgaaataact aaatttctaa gtaaagaaaa
    66901 cctgtgaata tgttgattat tatgtggata actattaatt cgatatttat ttatatagta
    66961 tgaataattg aataatatat ctaaaaaaag ccatcctatt attaagtaaa cttattgaaa
    67021 ggatggcttt ttatgaatag aaaattgtgc aaagcaataa tacctttatt tatttctatt
    67081 ttttgcatgt taataataag tataaaagct gaccaaggat atcaaaagaa taatgacaga
    67141 gcaattactg tcatgaataa taatgatact gaagatgatg ctgaaaaaaa acaagaaata
    67201 gactatgaaa aaacatgttg gaatttgcaa tctttgttta aaagtgatga ccaatggaaa
    67261 aaagaactta agagttttga taaagatact aaagagttga aaaattacat aggtaaagta
    67321 acaaaatcgc caacacattt gtcttttgcc ttaaacatta aagaaaaact tgatataaga
    67381 ctaaataaac tttctgccta tgtaaaatta aaacaagata ctaataaaaa ttcgtataag
    67441 tacttagata tgaatgatag tatgagtaaa tcctatggga attatatggg gatttgttca
    67501 gatttagagt tagaaatact aaaattatca aacaaagatt acaagaaaat tatttcaaat
    67561 aaaaatataa ataggaaata tggtgcatat ttaagagata taagaaggaa taaagtacat
    67621 tatttggatg ataaatcaga agatattcta agcaaagtat cttcaataag tagtcttcca
    67681 agccaagtgt atgaattatt tagaaatatg gatagaaaaa ctaatttgac accagctcaa
    67741 tatgcgagtg aattagagtc atcagataga gaaaatagaa aaaaagctta tcaagatgaa
    67801 cttataactt acaatgataa tataaataca attgcaggtt taattactgg tcaagttaaa
    67861 aagaatattt tttattctca ggaaagaggt tatgagtctt ctttagaaat gtacctggaa
    67921 gctgataatg tagatgaaaa agtatacaat aatttgataa ggactgtaaa tagtaatatg
    67981 gacagtctac acaaatatat agatttaaga aaaaaagtat taaaattaga taaggttcat
    68041 tcatatgata tgtttgtgcc tatggttaag gctgttgata ataatattaa ttatgaaaag
    68101 gcacaaagta tagtatattc agcgttgtca cctttaggaa aagaatacaa tgatgtagta
    68161 tataaagctt tcaatgaaaa atggatagat gtttattcac atgaaaataa agtaagtggt
    68221 ggatactgct tatctgttta tgaaaatcat ccatatatac ttttaaatta taataattca
    68281 ttaggttcag tttcgacttt atcacatgag cttggacatg ctgtatatga atacttaagt
    68341 tctaagaatc agaattattt taattcaaat ccgtctatat ttactcacga agttgcatct
    68401 acaacgaatg aagcattact ttacgagatg cttataaaag aagctaaagg caatgatgaa
    68461 aaagcttatt atataacaca gtatttagac ttgataaaag atactcttta tacacaaact
    68521 atgtatgctg aatttgaaaa aactatacac gagttagtag aaaaaggaaa aagtgtaaac
    68581 gcattagtat taaatgatgt ttggggacaa cttcttaaaa aatattatgg acaaagctat
    68641 gaattagacc aattatctaa ggtcggatgg gctagaatcc cacattttta taatagtttc
    68701 tatgtctata aatatgctac aggttgtagt gcaggagtaa gttttgccca agacatccta
    68761 aagaatggtt cagataacta tatgagcttt cttaaaaagg gaagttcaga ttatccaata
    68821 aacttgttaa aatcaagtgg cataaattta actaatacaa aacccataga aaatacaatc
    68881 aaaaaatttg atagtttagt agcagaactt gaaaagctat tataaacatt attgtaatcc
    68941 acatggtaaa tatatcgaat taatgataaa atatactaaa agtttaaagt cctatcaaga
    69001 taggactttt tatttattaa ttagtgggag atgattctat gcaagttaaa gttaaaaagg
    69061 ctgtaatacc agcagcaggt ttaggaacta gatttttgcc agctacaaag gcacaaccaa
    69121 aagagatgtt accaatagtt gataagccaa ctctacaata cataatagaa gaagctgtgg
    69181 cctcaggaat agaggaaata ctaataataa caggtagaaa taaaaaatct attgaagacc
    69241 actttgataa atctgtagaa ttggaacttg atttagagaa aaaaggtaag aaagaattgt
    69301 tggaggtagt acaaaacata tctaacatga ttaacataca ttatataaga caaaaagagc
    69361 caaaagggtt gggagatgca atttattgtg cgaggcattt tataggtgat gagccgtttg
    69421 cagtaatgtt aggggatgat attgtagata atgatgtacc ttgtctaaaa caactaacag
    69481 atgcatatga ggaatataga actacaatat tgggtgttca aaaggtaaat caagaagata
    69541 ctaataaata tgggataata gaagcaaaaa atatagaagg tagagtttac aaggtaaaag
    69601 acatggtaga aaagccagag tcaggaaaag ctccttcaaa tattgctatt ttaggtagat
    69661 atataataac tcctgaaatc tttgatattc ttaaggattt accaccaggt aaaggtggag
    69721 aggtacaact tacagatgct ttaaaaatat tatctaagaa agaagctatg tatgcataca
    69781 attttgaagg caagagatat gatgttgggg acaagttagg atttttagaa gctactgttg
    69841 actttgctct aaagaaagaa gaccttaagg aagattttat aaaatatttg aaacatgtat
    69901 gtagtgaatt tgataaaaat aacaatgtgt tatacaataa tacagatgaa aaaatagatt
    69961 taattgaagt caatgaagct agagaacttg aaattcaaaa ataattaatg aaaggtgatg
    70021 tatggtggca aaatcaagtc agaaaagaat taatagaata atagctgttg ggattgtggg
    70081 aatcctcgta ataacgagta taacagctgt tttgagtatg attgcaacta tgtaataaga
    70141 taaatgacag cagttgatat acataaatat aaaaaccata tataacttga tgtagtcaaa
    70201 aacttacaat attttagtta tatatggttt ttag
//
LOCUS       DAFKMX010000002        51518 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFKMX010000002 DAFKMX010000000
VERSION     DAFKMX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.002"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            140..352
                     /locus_tag="LZB08_000065"
     CDS             140..352
                     /locus_tag="LZB08_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313589.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(652..1845)
                     /locus_tag="LZB08_000066"
     CDS             complement(652..1845)
                     /locus_tag="LZB08_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7313590.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLGKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYNKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(1925..2128)
                     /locus_tag="LZB08_000067"
     CDS             complement(1925..2128)
                     /locus_tag="LZB08_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBM7313591.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            complement(2549..2806)
                     /locus_tag="LZB08_000068"
     CDS             complement(2549..2806)
                     /locus_tag="LZB08_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313592.1"
                     /translation="MNGTNGNQNDYLENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            complement(2787..3227)
                     /locus_tag="LZB08_000069"
     CDS             complement(2787..3227)
                     /locus_tag="LZB08_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7313593.1"
                     /translation="MEPNRREFQKQCVFQSFCKRVLHNEACNAHDEIRRRRKREVSFC
                     DLALHEERQLYTTDKYFQAETAEPTYQMAGKEITPKLLLEAIRTLPEEKRTAILLYYF
                     EGMTDVEIGKMFNTSRSTIQYRRTSSFELLKKYLEEHADEWNEW"
     gene            complement(3694..4467)
                     /locus_tag="LZB08_000070"
     CDS             complement(3694..4467)
                     /locus_tag="LZB08_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7313594.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGPTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGAEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKEPGTLTVGKKKKAVRKKGW"
     gene            complement(4473..5282)
                     /locus_tag="LZB08_000071"
     CDS             complement(4473..5282)
                     /locus_tag="LZB08_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7313595.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLAFLLPCLVGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSIAFCLISTLSVALFCKLFHVGMVYGMAYKIYMSLIFGVLIVAASL
                     PIVVLIIYFNKSFLLSILLAFFYSIFNWGLMGTIGTSISAAKIAFLNSFPVMCVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTFHTIFIMAITVVLSLWLIIRFYKKWTR"
     gene            complement(5275..6201)
                     /locus_tag="LZB08_000072"
     CDS             complement(5275..6201)
                     /locus_tag="LZB08_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7313596.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGALLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            complement(6338..7255)
                     /locus_tag="LZB08_000073"
     CDS             complement(6338..7255)
                     /locus_tag="LZB08_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7313597.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGTGTEFTLLFPLAN"
     gene            complement(7262..7954)
                     /locus_tag="LZB08_000074"
     CDS             complement(7262..7954)
                     /locus_tag="LZB08_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7313598.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNMGKEGLQKLKER
                     EYQLVILDVMMPGMDGFETLEEIRKESSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIVSLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            complement(7947..8135)
                     /locus_tag="LZB08_000075"
     CDS             complement(7947..8135)
                     /locus_tag="LZB08_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313599.1"
                     /translation="MEYMSCPEAAKKWGISERRVQILCREKRIQGVSKLGYMWLIPKD
                     AEKPIDGRTKQGKELYHE"
     gene            8297..8650
                     /locus_tag="LZB08_000076"
     CDS             8297..8650
                     /locus_tag="LZB08_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313600.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPTQKDTKRRQLDALLDDMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            8899..9228
                     /gene="mobC"
                     /locus_tag="LZB08_000077"
     CDS             8899..9228
                     /gene="mobC"
                     /locus_tag="LZB08_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBM7313601.1"
                     /translation="MNGRKRKVQIKFYVTEEERVLIEQKMKLVPTQNMAAYLRKIAID
                     GYIIQTDHSDIKAMTAEIQKIGVNINQIAKRVNATGSVYQEDIEEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            9189..10538
                     /locus_tag="LZB08_000078"
     CDS             9189..10538
                     /locus_tag="LZB08_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBM7313602.1"
                     /translation="MAVTKIKPVKSTLSKALDYIQNPDKTDGKTLVSSFGCSYETADI
                     EFEYTLSQARQKGNNLAFHLIQSFEPGEVDCEKAHAIGKQLADAATKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKVGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRTKAAKAPKEQRGVSLLIDIENSIKAQESRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEEITAENEKTADALKSVEKRLADMA
                     VLMKHVTTYQKTKPVYEAYRRAKDKDAYRAKNESSLILHEAAAKTLKASGVTKLPNLA
                     AMQEEYGKLQAQKEALYTDYGKLKKQVKEYDVIKQNIDSILRLEKEPERSRETERLQ"
     gene            10599..10868
                     /locus_tag="LZB08_000079"
     CDS             10599..10868
                     /locus_tag="LZB08_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313603.1"
                     /translation="MADQTIPDYETIRAAVAGETWAVEKVLMCYKDEIDRQATVKKRQ
                     PDGTFKLEIDEDMRQYITMKLIEALPQFPLEEMEREEKRNRDKKS"
     gene            10915..11208
                     /locus_tag="LZB08_000080"
     CDS             10915..11208
                     /locus_tag="LZB08_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313604.1"
                     /translation="MKQRQKETISTWLYEEYRRLWIEKGQEPHKRNNREIVAAVMEQI
                     EAAGIWLPEQEVTKYFYRRKTHYRNRIEKELQPKPEMQDKDSQTQKEQGAGSQ"
     gene            complement(11353..11718)
                     /locus_tag="LZB08_000081"
     CDS             complement(11353..11718)
                     /locus_tag="LZB08_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBM7313605.1"
                     /translation="MTGSKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECVCPQ
                     LISYSLLCKWFQTAVLPLDKLLYAELFKTEDKKRCTECGTFFVSKSNSVKYCPDCRKR
                     ITRRQAAERMRKRRAAVTQ"
     gene            complement(11715..11921)
                     /locus_tag="LZB08_000082"
     CDS             complement(11715..11921)
                     /locus_tag="LZB08_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBM7313606.1"
                     /translation="MIRLTVEEMNLLSIYHEGSKAQLMENMTTALPFMDEDMRPFAER
                     TLQKISPLTENEYAELSIFAADEV"
     gene            complement(11924..12868)
                     /locus_tag="LZB08_000083"
     CDS             complement(11924..12868)
                     /locus_tag="LZB08_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313607.1"
                     /translation="MKLQKFENVDVIASLEAIMKQNTAFYQSDFDIDKQILQKAAVSP
                     VPEDKRLLWFSRPSGTCCFRERDVFLKDTRQHNTWRFYGEQTRDTILAYAVELTGTEQ
                     EKIKGNLYELDYLQHFREVIEKSIPADNYTLIYEHGELTKPAGQYFDGNTDPQLGKFI
                     RFEAQPNDPEALKSILREQQKKRTPHTQGDFQLHIAALHDRRIEAEARRIVENVKGLG
                     EPDSPEKTHCAVELSPYFVPLATDKDMERLLSMLPYKSLSFSTLEGRHGVYALIDKSE
                     RNKEMKKSRPSVRAQLQTEKSPKLPKKTAKSKHHELEV"
     gene            complement(12872..13474)
                     /locus_tag="LZB08_000084"
                     /pseudo
     CDS             complement(12872..13474)
                     /locus_tag="LZB08_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
     gene            complement(13802..14008)
                     /locus_tag="LZB08_000085"
                     /pseudo
     CDS             complement(13802..14008)
                     /locus_tag="LZB08_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14990..17257)
                     /locus_tag="LZB08_000086"
                     /pseudo
     CDS             complement(14990..17257)
                     /locus_tag="LZB08_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            complement(17499..19589)
                     /locus_tag="LZB08_000087"
     CDS             complement(17499..19589)
                     /locus_tag="LZB08_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBM7313608.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIVSWCVGHLV
                     GLAEAAAYGEQYKKWSYDSLPILPQEWKYTVAADKEKQFKTLKELIHRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLA
                     LSGAEAASERISDKAEADALKTTCEASRTVCTSLVKEKKTAAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSAFLTDDMGDTAAGIIKLLCGKFSFMAGKD
                     FTPELAKVLNSKKVSDHHAIIPTMELSKTDLAALPESERNILTLAGARLLMATAAPHT
                     FEAVTAIFECAGQSFTARGKRVLSDGWKELDRRYRAALKNKPETDDADSDTEKTLPPF
                     TEGQTFENSAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKAEP
                     AAFMEGIEDMARELVKTYPFLSEADKGRFKEEKPELGKCPRCGSPVYEGKKNYYCSNK
                     ECIFTMWKNDRFFEERKVTFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRIELPKKK"
     gene            complement(19710..20030)
                     /locus_tag="LZB08_000088"
     CDS             complement(19710..20030)
                     /locus_tag="LZB08_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HBM7313609.1"
                     /translation="MASSKEYLEFILGQLSELEEITYRAMIGEFIIYYRGKIVGGIYD
                     DRLLVKPVKSAISYMPTAPYELPYEGAKEMLLVDEVDNKEFLTGLFHAMYDELPAPKP
                     KKKK"
     gene            complement(20299..21027)
                     /locus_tag="LZB08_000089"
     CDS             complement(20299..21027)
                     /locus_tag="LZB08_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBM7313610.1"
                     /translation="MKNKLKKTLTALCAALILMGGFSVPAYAQGAATEPPAEDATNDS
                     GVIVEEKEETPPLTPKGNATLVDDYGGNKQLITVTTKSGNYFYILIDRAAEGEQTVHF
                     LNQVDEADLMALTENGEAAKKPESCNCTEKCEAGKVNTSCPVCVTSMTGCTGKEPTPT
                     PTEQPEEPKEKGGNPGVVLALVLFALLGGGAAFYYFKFLKPKQNVKGDTDLEDFEFED
                     YDEDEPETDTGETPEDEETEEETL"
     gene            complement(21017..21286)
                     /locus_tag="LZB08_000090"
     CDS             complement(21017..21286)
                     /locus_tag="LZB08_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBM7313611.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRLTPQELNAMLSGGGIPGMNAAPATEAADYEEQEENADEE"
     gene            complement(21318..23258)
                     /locus_tag="LZB08_000091"
     CDS             complement(21318..23258)
                     /locus_tag="LZB08_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBM7313612.1"
                     /translation="MTRDGVVEVNETQQTAERISSREADSDFSQPDSAAAERVIEHLD
                     AAHTRKANKKAVKKEQEATALRTSTSRLQFTEEELAAPELERYIEKSNKAADRLDAAK
                     AAIPKQKKLVKERTFDEATGKAKTRLLFEKQEKPIPGGKKGNPLSRPAQEAGIFVHNK
                     IHSVEKDNSGVEGAHKSEELAERGAKYGARKLKQGYRSHKLKPYREAAKAEKAAFKAN
                     VNFQYHKTLHDNPQLTSNPLSRFMQKQQIKRQYAKAAKKGGAKAAAEATRKTAKKTAE
                     ETRKAAAFAARHPAGILIAVAALLLFIMISAGLSSCGAMFSGLTNGVLGTSYTSEDSD
                     LLAVENNYAALENGLQSEIDNIENTHLGYDEYRYDLDSIGHNPHELASYLTALLQSYT
                     PQSAQAELERIFAMQYTLTLTEEVEIRYRTETSTDPETGETTTEEVPYEYYILNVKLT
                     NKPISELAEELLTPQQLEMFHVYLETSGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRP
                     GNQELMELAKQQVGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQ
                     GVPWFQSRGQWGARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGD
                     ACKIKSYDLNYQCIKGYGLMNW"
     gene            complement(23285..24244)
                     /locus_tag="LZB08_000092"
     CDS             complement(23285..24244)
                     /locus_tag="LZB08_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBM7313613.1"
                     /translation="MKTDVIINRDALCALKELPSESVHCCVTSPPYFALRDYGLDAQI
                     GQEDTPEQYIDRLTSVFRELYRVLRKDGTLWLNIADTYCGTGNKGGYADPKNPKGRTG
                     QRIARNSRVTGCKQKDLIGIPWLLAFSLREQGWYLRSDIIWQKQNPMPESCKDRPTRC
                     YEHIFLLSKEKKYYYDAAAIAEPLAPTTAERYRRARSTNSKYTQEIPGQGKVQGLNRP
                     RDGGYYDDALMPTTRNKRDVWLINTVPYKGAHFAAFPPKLAETCILAGCPKGGIVIDP
                     FFGSGTTGFAAKSLDRHYIGIELNAEYCALARARIGGAGLSNN"
     gene            complement(24241..26670)
                     /locus_tag="LZB08_000093"
     CDS             complement(24241..26670)
                     /locus_tag="LZB08_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7313614.1"
                     /translation="MSKGQTKKTREAAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKVAQHKYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKSQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDVLNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEIFAPELNDRILADMLD
                     LETGVIVNLHIRSIDQSEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFSAKREITNAFLITDDDIFICDPEAEYFALVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYRPFLADPDPAKMPILGDLYDELLRQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELNNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            complement(26618..27022)
                     /locus_tag="LZB08_000094"
     CDS             complement(26618..27022)
                     /locus_tag="LZB08_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBM7313615.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSVALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGSRRKQA"
     gene            complement(27064..27933)
                     /locus_tag="LZB08_000095"
     CDS             complement(27064..27933)
                     /locus_tag="LZB08_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313616.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFSMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            complement(27952..28167)
                     /locus_tag="LZB08_000096"
     CDS             complement(27952..28167)
                     /locus_tag="LZB08_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBM7313617.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(28431..28598)
                     /locus_tag="LZB08_000097"
     CDS             complement(28431..28598)
                     /locus_tag="LZB08_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313618.1"
                     /translation="MSRQKTKTDDIRREHYFTKMIENAENALREDLQERGGEQTLYVT
                     EYMQRRSYLLE"
     gene            complement(28618..28785)
                     /locus_tag="LZB08_000098"
     CDS             complement(28618..28785)
                     /locus_tag="LZB08_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313619.1"
                     /translation="MNKRIRKKKDKKITEAINGLVFIAERREQQRQAAIRQFVKRCEA
                     LYEQKRVRREL"
     gene            complement(28778..30568)
                     /locus_tag="LZB08_000099"
     CDS             complement(28778..30568)
                     /locus_tag="LZB08_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBM7313620.1"
                     /translation="MNAINWKKLLLPNIPYLLFVYLFDKVGQAVHLAPGADLSGKVLS
                     LGEGFSAAFANPLPSLAPMDLLIGILGAVLIRLIVYVKGKNAKKYRKGVEYGSARWGT
                     AEDIKPYTDPVFQNNVLLTQTERLSMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMKYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKPDEVFDYYEISESDLQEDTDHE"
     gene            complement(30565..31047)
                     /locus_tag="LZB08_000100"
     CDS             complement(30565..31047)
                     /locus_tag="LZB08_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBM7313621.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDKQKI
                     PHGKQTLKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTETPPRYLVFF
                     KGRDADALTAAFKEFSAKKLTQEQNPSIRKLIVSLKEKAAALNAQRDKVKNKDRGIAR
                     "
     gene            complement(31142..31360)
                     /locus_tag="LZB08_000101"
     CDS             complement(31142..31360)
                     /locus_tag="LZB08_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBM7313622.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            complement(31385..32329)
                     /locus_tag="LZB08_000102"
     CDS             complement(31385..32329)
                     /locus_tag="LZB08_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313623.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPILENPTADNPTSENPTQLNKELLKTNLPNKEKLNTDISSTHSIPFHSLNPSPLED
                     AAQPPERKRKETTDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILALILETVCTKR
                     RTIRIAGDDHPAELVKAKFMKLNSEHIRFVLDCMQENTTKIRNIKQYMKAALFNAPST
                     IGSYYTSLVSHDMADGTFYGKPKKSGIPDYTCDEGESL"
     gene            33004..33174
                     /locus_tag="LZB08_000103"
     CDS             33004..33174
                     /locus_tag="LZB08_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313624.1"
                     /translation="MSALLYGVCNKPISISIVTNMQEFKIGVSMDYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            33717..34595
                     /locus_tag="LZB08_000104"
     CDS             33717..34595
                     /locus_tag="LZB08_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBM7313625.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            34631..36526
                     /locus_tag="LZB08_000105"
     CDS             34631..36526
                     /locus_tag="LZB08_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7313626.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            36794..38701
                     /locus_tag="LZB08_000106"
     CDS             36794..38701
                     /locus_tag="LZB08_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7313627.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDELMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            38691..39140
                     /locus_tag="LZB08_000107"
     CDS             38691..39140
                     /locus_tag="LZB08_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7313628.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            39240..40127
                     /locus_tag="LZB08_000108"
     CDS             39240..40127
                     /locus_tag="LZB08_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7313629.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            40532..41980
                     /locus_tag="LZB08_000109"
     CDS             40532..41980
                     /locus_tag="LZB08_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBM7313630.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIKTDGEILFNDMETHA"
     gene            42006..44396
                     /locus_tag="LZB08_000110"
     CDS             42006..44396
                     /locus_tag="LZB08_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBM7313631.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSDITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDVSRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            44398..45069
                     /gene="pgmB"
                     /locus_tag="LZB08_000111"
     CDS             44398..45069
                     /gene="pgmB"
                     /locus_tag="LZB08_000111"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBM7313632.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLA"
     gene            45108..46508
                     /locus_tag="LZB08_000112"
     CDS             45108..46508
                     /locus_tag="LZB08_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7313633.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKEGIDNVVEEV"
     gene            46825..47667
                     /locus_tag="LZB08_000113"
     CDS             46825..47667
                     /locus_tag="LZB08_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7313634.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            47812..49740
                     /locus_tag="LZB08_000114"
     CDS             47812..49740
                     /locus_tag="LZB08_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7313635.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            49801..51228
                     /gene="ascB"
                     /locus_tag="LZB08_000115"
     CDS             49801..51228
                     /gene="ascB"
                     /locus_tag="LZB08_000115"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBM7313636.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
BASE COUNT        15444 a         9740 c        10829 g        15505 t
ORIGIN      
        1 ctttgcaaca ccttatacaa gtaatataaa tatatcaaac ctagatacag gtataatcct
       61 cattgcagtt ttatttatac agtctttatc taaattagtg ttgtatatat actttgataa
      121 aagggggata tgtaactaag tggcaatatt gatgaaaaca aaaattaaag aatacagaga
      181 aaaactcctt atgacgcaaa atgaacttgc taaattggtt ggggtaagac gtgagactat
      241 tgtacactta gaaaatggga aatataatcc ttctttgaaa ttagctatgg atatcgctaa
      301 agtattcgat actacagtag aaaatttgtt tgaatttatt gaagaagaat agtggaaaat
      361 ttataaacat ttataagatg gataataaaa tatagttatt taattattaa gtttgttaaa
      421 aatatttata taatataaca tgtgattaag gtcagattat aattgtttta ttaaaaatca
      481 caaagtaatt tttgattatc ttttcaaaag ttcttatatg ccggtatagc ctattgttac
      541 aggcttttcc ggcatttttc attgtggaag atactctcaa atcttcttat atccccgtat
      601 gttttggtgt tcaaaagtag tatgtggagt agtaaattct ccgtttacta cttaggcaat
      661 cagccgcttc atttctgtta tcgacgtttc ggaagtcgta tgcgcgtaat agttcagcgt
      721 catattgatg ttggaatgtc ccatgatgta ctgcaatgcc tttgggttca ttcctgcatt
      781 tgctaacgtg gtgcagaatg tgtggcgtaa ggtgtgcggt gtcattacct ttggcaatgc
      841 taccttttgc gttttgttgt atttccttac aagcccccgg aacatacttt catagttgac
      901 tgccactttt ggcaaaccgt tccggttaag gaacagaaac tttgtgtagc cctctaaaat
      961 aacaggctgt gcatatttcc gcttgttcag cacccgctga aatgcttcta acactttttc
     1021 actcatagga ataacccggt tgccgctttt cgtcttaggc gtttccacat agtagcccac
     1081 gtctgcaatc ttcaaaagtt ggtggtctac attgataagc tgtttgccta agtcaatgtc
     1141 agcttccgtc agtccgcaga gttccgaaat gcgaagccct gtccctaaca gtatgataat
     1201 ctcgtcgtag tatttctgat agactttatc atgctgcaca aaggacagaa aagccgcttc
     1261 ctgcgtaggg gatagaggtt cctttggttc cgtatcatct tcaaggactg tgttcaactg
     1321 gaagtcaaat ggatttttac gaatacaatc gtcctgtatg gctgtgtaaa aggcagcctt
     1381 taaggaacgc ttgtggttgt tgatagtctt gaagccgtaa cctttttctt tcatgcgtaa
     1441 cgcccattcc tttgcgtcgg agagcttcac attttcaatc cggcaggctc caagtttatc
     1501 ctcctgcaaa atccgcatga gctgttttcg cccctgcttt gtaccatgcc gcacatttgc
     1561 ccggtggcgt atctgctttg cgtagagttg gcagaccgtc attttctttc cgatatggtc
     1621 gataccgtcg tcaaggtctt tttggatttc tttctctttt tccctcaacg ctatatcgtc
     1681 gcgctttcct gccggggttt tgtccgtagg cactaacttc caagaataca caaactgcgg
     1741 tttcccaaag gtatctatat atttgtaggc atatctcccg tcttttctct ggctctctcc
     1801 ggtgcggaga atacgatttt tattatcccg tcttttttcc gacatcgtta tgctcctttc
     1861 cataatggaa agagccttga tatgcttgac tatattgtac cacattcaag gctcgatttc
     1921 actatcagat tgcgtccacc gtatcaatca ttttttcaaa ctgcttacgc ttaatctgaa
     1981 tacggtttcc gttcatcatt acccagtccg ggacgggatt ttcttctgcc agtttccgca
     2041 gcttgctttc tccgatacgg aaatattttg ctgcttcttc tatggtaaga gtgtactttt
     2101 cccaaatcgg cacgtcgtta ttgttcaatt tgtcgtcccc ctttcttaat gggtcaaaaa
     2161 tcaatctgta acataggctt taatcggacg gctattagcg taacctcatg ggacttgcac
     2221 ccctccggcg gtctgccgaa gccctaccca ttgcctgcga cgctcctaac gctcggacta
     2281 tggctataag gaagtatcat tatgtattct gtgcgttatc gcccgcaggc tgctatctct
     2341 gctttacggt gtggtatttc tcgctcgctt ttctattaag agaaaggtca tggcgtaccc
     2401 gccgcatggc tcggcacaaa aggaacgtat ccgatttgcc ttatactgcg tcgccgttct
     2461 attgcgccgc tttctttatc aaagagcaga gggctgttct gcgatatgga acggaaaagg
     2521 ggaaagaagc gttccaatat cgcataggtt attcagcccg aaaagtgagg atagccctca
     2581 tcagtcttgc ttgcagccgt tcccgaatat cctcgtcaac gccataatag acattgccgc
     2641 gctcgtcgta gagcttccgc atggaaagac ttgctatgta gccgctgaaa tgctgcaaga
     2701 ccattttcat agcgtccggg tcgcctttgc tcgccgccaa aataacggga tagggaacaa
     2761 gggcattttc caggtagtcg ttttgattac cattcgttcc attcatcagc gtgttcctcc
     2821 aaatatttct ttaacagttc aaaagagctt gtccgtctgt actgtatcgt gctgcgcgac
     2881 gtattgaaca tctttccgat ttccacgtca gtcatgccct cgaaatagta cagaagtata
     2941 gcagttctct tttcttccgg cagggtgcgt attgcttcaa gcaataactt cggggttatt
     3001 tcttttcccg ccatttgata agtcggctcg gctgtttcag cttgaaaata tttatccgta
     3061 gtgtaaagct gccgttcctc atgcaaggca aggtcacaaa aagaaacttc ccgtttcctg
     3121 cgtcgccgga tttcatcatg ggcgttacag gcttcattgt gtaacacccg tttacagaaa
     3181 ctttgaaaga cacattgctt ctgaaattct ctgcgattag gttccatgta atcacctcct
     3241 ttctgccgaa aggcggtagt acctcccctt ttcgcgggat aatacaggcg actttttgct
     3301 ttgccaaaaa gagagcaaag tttttgaaaa atcttctgta aaatgcaaaa tgcgcccatc
     3361 tgcaaggcgc agaccgacgc ataggaaata acagcagtat ttaattgatt tgatagttca
     3421 tctgtatagt cggacttgtc cggtggggga gctatacttt ttttaatgaa tgtaacctat
     3481 gtatgctgta tagcaacaaa gaaatcccca tagcggcagc cctcctaaat gccgctaatt
     3541 tatgttatag gttgtcgtga tttagcagta gacaaaaaaa gcggcggctc taaaaatcaa
     3601 agccaccgct tcaaaggcgc gtgaaagtgt atctgctctg cgacagcttt ttttcttttg
     3661 ccgtcgttcg gcaggtacta tcaatcaaat tatttaccaa cccttttttc taactgcttt
     3721 tttcttctta ccaacagtca atgtaccagg ttctttcttt ttatttgcaa gaataatcat
     3781 agaaatcaag aaagtcaaaa ctattgtaat gaaagcgggg aaaatgtgat aagtataacc
     3841 atattccgca ccacaagtag ggtctactaa aatcatgctt gctttaatcg gataataatt
     3901 taacagtgtt ccttcaaaag ttccaaaata tccatacaca aaagctagtg caactccacc
     3961 caaaaaatta tttgccgaac aacagaatat cagaataatc ggcaagactg aaatataaat
     4021 tcctatattc gccccagtaa cacgaataaa catattgagt agattgccaa aatgaactcc
     4081 cggaaatccg acaataatat tgattactaa tgctatcaca caacctataa gactaaagct
     4141 gattgtcaac agcaatagaa taagtaattt tccagaaagt aactgtttat atggaatagg
     4201 aattgttaat atattcttca tagtatcgtc tgtatattcc cttgttatga tataacctgc
     4261 aatcagtgca ataataatcg gaaaaaataa ggtacaatta ctaatcataa cctgttgcat
     4321 aaaatagtca aacgtccatg tcggtccacc gttggctgtg gaataaaaca gtgacagcaa
     4381 cggagaaagt gttgtcataa taattccggc ttttattaca gaatatcgtt ttaattttaa
     4441 aaattcagtt tttattatat ttctcatatt ttttacctcg tccatttttt ataaaagcga
     4501 attatcagcc acaaagaaag tacaactgta attgccataa taaaaattgt atggaaagta
     4561 gttggtacaa tcgcataggc ttccggcaaa agattatctt tttgcaaatt atccatcatt
     4621 aagccgcttg tccagttcat aacacacata actgggaaag aatttaagaa agcaattttt
     4681 gcagcactta tagatgtccc aatcgttccc attaagcccc agttaaaaat agaataaaaa
     4741 aatgcaagca ggatagaaag caaaaaactt ttgttaaagt aaataattaa aactacaatc
     4801 ggcaaagaag ctgctacgat aagaactcca aaaataaggc tcatatatat tttataagcc
     4861 ataccataaa ccattcctac atgaaacagt ttacagaaca atgcgacaga taatgtgctt
     4921 atcagacaaa atgcaatgct gaaaataaag agcatagaaa ttttcgccat aatgagctgt
     4981 gtatttgtaa caggaattgt tctaaggttt ttggaagtat cgcaatctct ttctataaaa
     5041 aacaaaatag cggcaataat accaactaga catgggagca aaaaagctaa gccatatccc
     5101 aacatcattg tataaacata atcaaatcgg gtttgaagat aatgttccgt tgtatctgca
     5161 cctgttcccg cctttgccat atatgccaaa atcaacggaa aaaataacga taacgacatc
     5221 agtgcatata ataatctttt ccgttttaat ttccaaaatt cgcatttgat tagtttaagc
     5281 aattccctcg cctcccgtca cacgcttgaa gtaatcttca agactttctt cacaagtatg
     5341 ggcttctgat acctccaatc cattttctac aaaagcagtc acgatttttc ctacggacaa
     5401 atcaagatta tgcaagcgta aattgtggtc gtcctgtata gagaaatggc tctcatggaa
     5461 agtacgctct aaaattcttg ctgcctgtgc ggtatcagaa agagtaaatc ggatatgttt
     5521 gctgcttttt tgttccagtt ccgcaagact ttcttcttcc aatagtgcgc catggtcgat
     5581 aattccaata tcatctgcta gcaaagaaat ctccgaaagg atatgactgg aaattaaaat
     5641 tgtttttccc tttgtatcgc aaagctcgcg aataaaagaa cgtacttctg caattccaat
     5701 cgggtcaagc ccattgatag gttcatctaa aatcagaagt tcagggtcgt gcataactgc
     5761 aagtgcaatc gcaagccgtt gtttcatacc aagagaatac tgggaaaaca gttttttgtc
     5821 tttataaggc aatcctacca aatccaatgt gtttttaatt gaatgacggt tgggtatgcc
     5881 ccgtaaggta gcaaaaattt gtaagttttc cgtagcagtc aaattagggt aaaaaccggg
     5941 cgactcaatc agactaccga tacggggcaa caatttcttt tcattcccct gcaaagactt
     6001 tccccatatt ttaacttcgc cggaagttgg ctttgtcaat ccaagtagca ttttcatagt
     6061 ggtcgttttt ccggctccgt ttcttccaag cagtccgtaa attctccctt tctgcacatg
     6121 aatatttaag tcagcgacac tcttttgtga gccgtactgt ttggtaagat tttttgtttc
     6181 aatgatataa tttttgtcca tattcaaacc tcctattctg taaagtattc tatcatacca
     6241 accttgcatt aaccttgccg caaccttgca ttaaccttgc aatttgaaat attgtcctaa
     6301 aaataattaa ggctcccgtc aaaaggacgg gagaacatta atttgctaag ggaaaaagca
     6361 aagtaaattc cgttcctgtt ccctgtatgc tatttgctgt tattgtgccg ttcatttttt
     6421 ctacaagttg gtgtgcgata gaaagcccaa gaccgctgcc tttttcagac cgtcccttat
     6481 cgcatttata gagccgttca aaaatatgct tcaaatcttc tttttcaatc cctattccat
     6541 tatctgctaa tcggatttgc atattttttt cctgctttga caggattatt tctattttat
     6601 ccgcatgact gtgagaaatt acattctgaa taagattgtt caatatccgc atatagccgt
     6661 ccgtatcaag ttttacccgg aaaggctgtt cgggaatatc aatattgtaa tctatctgct
     6721 tatcttcaaa tatgggtatc cagtcaatca agatatttcg tgtcagctcc gcagcttcaa
     6781 caatatttat atccatagca aactcattag aatttaattt gaaccagtca aaaagaacat
     6841 caatatattc tttcaaatcg tgagctttgc ggcgggctgt ttcgatatag tcgtccctgt
     6901 cttttcctgt aacaattcct ttatgtgcag cgtcaagata cccaataagc gttgtaagcg
     6961 gtgtccgaac atcatgggaa agacttgtca taagctgtcg gtttgtttct tctgtctgcc
     7021 ggacagtaga aagcctgctc tcataagaca caacaatctc atttatttca taggcaagag
     7081 gggctaccag ttcatttgtt gcagataaaa tacgcctgtt tccatttccg ttttttacat
     7141 caatcaatgc gtctgttatc tcggctatct gttttttaac acgtcgaata gcaaagcagg
     7201 aaacaagaat agagaaaagt gcaattatta tggatagaaa aatgactgct tccatagaaa
     7261 atcaaacctc cttgttaaaa cgataaccga tacctttaat cgtttgaata tattttggac
     7321 ttccgggatt tacctctaat ttcttgcgaa gtcggctgat aatcgccata atgttgctgt
     7381 catcatagaa atattcctct ctccacactt cttcataaat ctgttgcttt gtcagaattt
     7441 tcccttgatt tttcgcgcag tataagagta agtcaaattc ttttggaggc aattcaaaag
     7501 ttccattttc cgtagtaaca gaacgatttt caatatcaat tttcaacccg tcaaattcaa
     7561 gttgctgtga tattccgtcc tgttgattga aacgggtata acggcgtata agggaaacga
     7621 tacgggctat cagttcgtcc atgtcaaaag gttttgtcaa ataatcatca gctcctgccc
     7681 ttaaaccgcg tactttagaa gcactgtcat tttttgatgt aaacatcaaa atcggcaggc
     7741 tgctttcttt tctaatttct tccaatgttt caaatccgtc cataccaggc atcataacgt
     7801 ccagtatcac aagttgatat tctctctctt ttaatttttg taatccctct tttcccatat
     7861 tacaaaaatc agcttctata tcttctgata gcacgctgcg tttaatcagc gcacaaagtt
     7921 ccttatcatc atctataatc aaaactttat tcatggtata gctcctttcc ttgtttggtt
     7981 ctaccgtcaa tcggtttttc cgcgtctttt ggtatcaacc acatataccc aagttttgaa
     8041 acaccctgta tgcgtttctc cctgcaaagt atctgtaccc gcctttcaga aattccccac
     8101 ttctttgctg cttcggggca agacatatat tccataacaa cacccgtcct ttctgaaaat
     8161 cttatcataa taattatagt cggacacacg aacaatagca agtatataaa tatgaattat
     8221 tatggctatt tagtggaact gtatagacat atccaaagag aaaggggaac agtaaaatgt
     8281 aactgggtga ataaatatgg caaaaagacc ggtagaaaaa tatgacttca aggcatttgg
     8341 agaagctata aaagctgcgc ggacggggcg caaagaaagc cgcaaacagg taagtgatga
     8401 aatgtatatt tctcctcgct atcttgcaaa tatcgaaaac aaaggacagc accccagtct
     8461 gcaaatcttt tttgagctta tgcttcgtta caatatatcc gtagaccagt ttcttttaga
     8521 tacgcccacc caaaaagata caaagcggcg gcagctcgac gctctccttg atgatatgag
     8581 cgataatggc atacggattg taacggcaac tgcaaaagaa atttcagaag tcgaaaaaga
     8641 ggacaaataa atgtgtccgg caaattgaat atgatttagg aagcgttgta atctttacag
     8701 attgcagcgt ttttcttttg ctcttttttg gcatatagag cattgcgttg tagtaaccag
     8761 tgaggaaaaa actttcgtag caagcatagg aaaagagtac ccttttccgt attttcgccc
     8821 tcccgtcctg cggtgcgtcg gttgaaaatt gcttgttggg aagtgtccca aaccctcaaa
     8881 gcggaaagga gcgattgcat gaacggacgc aaaagaaaag tacaaatcaa attctatgta
     8941 acagaggaag aaagggtgtt gattgagcag aaaatgaagc tcgtccctac ccaaaacatg
     9001 gcggcgtatc ttcgcaagat tgccattgac ggctatatca ttcagacaga ccatagcgat
     9061 ataaaagcca tgacagcgga gatacagaaa atcggggtca acatcaatca gattgcaaag
     9121 cgtgtcaatg cgacaggcag cgtctaccaa gaggacatag aggaaataaa gggggtgctt
     9181 gcagagatat ggcggttaca aagattaagc ctgttaaaag cacgttgagc aaagcccttg
     9241 attatatcca aaatccggat aagacagacg ggaaaacgct tgtgtcctct tttgggtgca
     9301 gctatgaaac ggcagatatt gagtttgaat ataccttgtc gcaggctcgg cagaaaggga
     9361 acaacttagc ctttcatctg atacaatcgt ttgaaccggg agaggtggac tgtgagaaag
     9421 cccatgcaat cggaaagcag cttgccgacg cagcaacaaa gggacagcac gaatacgtcc
     9481 ttaccaccca catagacaag gggcatatcc ataaccacat cattttttgc gcggtcaact
     9541 ttgttgacta ccataagtac aattccaata agcgcagcta ctacggcata cggaatatga
     9601 gcgacaagct gtgtcgggaa aacgggctgt ccgtcgtcgt tccgggcaaa ggcagcaagg
     9661 gaaaaagcta tgcagaatat caagcggaaa aggtcggtac aagttggaaa ggcaagctaa
     9721 aaattgccgt tgatacgctt atcccccaag tagcaagttt tgaagaattg ttgcagcggt
     9781 tacaggcagc gggctatgag ataaagccgg gaaaatatat ctcctgccgc gctcccggac
     9841 aggaacggtt tacccgctta aaaaccctcg gtgcagatta tacagaggaa gcaatcaaag
     9901 aacggattgc aggcaagcgc acaaaggcag cgaaagctcc caaagagcag cgtggcgtat
     9961 cgctgcttat cgacattgaa aacagtatca aggcacaaga gagccggggc tatgaacagt
    10021 gggcgaaaat ccataatctg aaactggcgg caaagaccat gaatttcttg acagaacatc
    10081 agattgagca gtacgcggat ttagtcagcc ggattgaaga aataactgcg gaaaacgaaa
    10141 agaccgcaga cgcattaaag agtgtggaaa agcgtcttgc agatatggcg gtgctgatga
    10201 agcacgttac cacctatcaa aagacaaaac cagtctatga agcataccgc agggcaaagg
    10261 ataaagacgc ataccgggca aaaaatgaaa gcagtctgat actccatgaa gcggcggcaa
    10321 agacactgaa agcgtcgggc gttaccaagc tccccaatct cgccgccatg caggaagaat
    10381 acgggaagct ccaagcgcag aaagaagccc tttacactga ttatggcaag ctgaaaaaac
    10441 aggtcaagga atacgacgtt atcaaacaga acatagacag tattttaaga ctggaaaaag
    10501 aaccggaacg gagcagggaa accgaacgct tacagtagaa atatctataa aagcgtggta
    10561 ggataaagat attccaatac agacaggagc gtgaaaacat ggcagaccaa acaatacccg
    10621 attatgaaac catacgcgcc gccgttgccg gagaaacatg ggcggtggaa aaggtgctta
    10681 tgtgctacaa ggacgaaatc gacaggcagg caactgtaaa gaaacgtcag ccggacggaa
    10741 cattcaaact ggaaattgat gaagatatgc gccagtatat cacaatgaag ctgatagaag
    10801 ccctccccca atttcccctt gaagaaatgg aaagagagga aaaaagaaat cgggacaaaa
    10861 aatcataaaa aggtagacgg gaagctgctg cggatagata agcagtttcg acatttgaag
    10921 caacggcaaa aagaaactat cagtacatgg ctttatgagg aataccgccg cctttggata
    10981 gaaaagggac aggagccgca taaacgcaac aaccgggaaa tcgttgccgc tgttatggaa
    11041 cagatagaag ctgcggggat atggctgccg gaacaggaag taacaaaata tttttaccgc
    11101 aggaaaaccc attaccggaa ccggatagaa aaagaattac agccgaagcc ggaaatgcag
    11161 gataaagaca gtcagacaca aaaagaacag ggtgcaggaa gccagtaatg acctcccgca
    11221 ccctgttctt ttataggtac tgattttaga atgttacgca gtagaacccc gatttcaagg
    11281 gaaaaggtat tctgtatcgc ctatgccagt ttcacgccgt ttatgccctg taaatcaagg
    11341 cttttttcgc ccttattgcg taaccgccgc gcgtcgtttc ctcatgcgtt ccgctgcctg
    11401 tctgcgggta atccgtttcc ggcagtccgg gcagtatttg acgctgtttg attttgatac
    11461 aaagaacgtg ccgcactcgg tacaacgctt tttatcctcg gtcttgaaaa gctctgcata
    11521 gagcaactta tcaaggggaa gtacggcggt ctgaaaccac ttgcatagaa gtgaatagga
    11581 aataagctgc gggcagacac attcctcccc gtcgtccaat aagatacagt gtccgcgctc
    11641 gcagttgcag cactcctttt ttacaaggct gttgactttc cggctctgtg gcggcgtgag
    11701 ccgcttactc ccggtcatac ttcatcagcg gcgaaaatgg aaagctccgc atactcgttt
    11761 tctgttagtg gggaaatttt ctgtaaggtg cgttctgcaa agggacgcat atcctcgtcc
    11821 atgaatggta gcgcagtcgt catattttcc ataagctgtg ccttgcttcc ctcatggtaa
    11881 atactcaaca ggttcatttc ttcaacggtc agtctaatca tgctcatacc tccaattcgt
    11941 gatgttttga ttttgctgtc tttttcggga gttttgggga tttttctgtt tgtaactgcg
    12001 cccggacaga gggacgggac tttttcatct ctttattcct ctcgctttta tctatcagcg
    12061 cataaacgcc atgcctgccc tccaacgtag aaaaggaaag gcttttgtaa gggagcatgg
    12121 aaagcagccg ttccatatcc ttatccgttg ccagtggaac aaagtaggga gaaagttcta
    12181 ccgcacaatg ggtcttttcg gggctgtccg gttctccaag cccttttaca ttttccacaa
    12241 tccggcgggc ttctgcttca atccgtctgt catgcagggc ggcgatatgt aactgaaaat
    12301 ccccctgcgt atgcggtgtt cgttttttct gctgttccct caaaatgctt tttaaggctt
    12361 ccgggtcgtt tggctgtgct tcaaaacgta taaactttcc aagctgcggg tcagtgttcc
    12421 cgtcaaaata ttgtccggca ggttttgtca gctccccatg ctcataaatc agcgtgtagt
    12481 tgtcggcagg gattgatttt tcgattactt cccggaaatg ctgcaaataa tccagttcat
    12541 aaagattgcc cttgattttt tcctgctctg tgcctgtcag ttccaccgca taggcaagga
    12601 tagtatcgcg ggtctgttcc ccgtaaaacc gccatgtatt atgctgcctt gtgtccttta
    12661 gaaaaacgtc ccgttcccgg aagcagcacg tcccggacgg gcgggaaaac cacaaaagcc
    12721 gtttgtcctc cgggacaggg cttaccgccg ctttctgcaa aatctgtttg tctatgtcaa
    12781 aatcgctctg ataaaaagcg gtattctgtt tcattatggc ttcaagggaa gcgataacgt
    12841 ccacattttc aaatttttgt aacttcatag gtcagctcct ttccaaatcc tgtgttttct
    12901 gtcttgcctg tttctttggt gtctgttctt tcgctgcttt aagctgcgcc cggatagagg
    12961 gcttttcctg tttggcaggc ttgccacgtt ccttatcggt tttcaatgct tcggcgtatt
    13021 ctgcaaggga aatctgtcct ccggcttttg ctttttcttc cagttccgca gcggtcgggg
    13081 tgttgtttat ctgcccgtca atcatgttgt aattttgctc ggtggtctgc tcggcagctt
    13141 tcaaatagtt tttcggctgc aaaaactctg gcacgtcctt ataccccaag ctgtctacat
    13201 agtgggcggt gtcctgcccg ttctgatgaa gcaccaccac gtcggaaacg gaaaggctgt
    13261 gtcccttaaa atctttcggg tggtcgatat tgaagcgggt gtaaatatct tcaagggaca
    13321 ttcccggcgt aaggggtgcg gtatagataa gctcataatt gcctctgtca atgctgtttc
    13381 ccgccgcctg tatgcggtcg taaggctcaa agcgcaagtc ccgcgtttct ttgccgttct
    13441 ttagctgata aattccgaaa gtgtcctcgt tcccatagag taagagagct tcccttgatg
    13501 gctcgctttc ccgtagttct tgcttcatgg cgttgtactc ccgcagggct tcccatgttt
    13561 ccttttccac gccgaacaag ccgccttttt gtgcgtgtgc ctgtaaatcc tccaaggtat
    13621 caacaagccc ctctgtgccg tcctcataga gaagataaac ggacatatcc tcatggaaaa
    13681 ggcgttcagc ggcttcctgc tgaagcggaa gcataccgtc ccaagcatag ccgtattcct
    13741 gcatatcctt gacgctgatt gtagggtctg gcatagggat ttccggcgcg gcggtctgcg
    13801 tctttgcgta aagctccttg acttctccct gtgtcagctc cccggcggta agctgcgcca
    13861 taagctctcg gcatttctcc ggcgttccga tatacaaaac gtcgcctatc tttgctttcc
    13921 cgtcctcctg cggaatgtag gcttgcagca gatacaggtt gtccctgctg tcgctgcggg
    13981 gatttgcgtg taccttgtaa atggcttcct gttcgggctt tttctgcgcc gcttcttttt
    14041 gcgcccggat atgcgcccgc tgtatctcgg taggcttttc cccggtaaag ggtgcaatcg
    14101 ccttgatata atcagccgca tagtaggcgt tactggtaag ctgccgctgc catgcccctg
    14161 ctttgggcga ccaacggaag ccattacttt ttaaggcttc ccgcgtggct tcgtccggct
    14221 tatcttcaaa gaagatttgc agacggttcg cttcggtgtt tgcttccacc ttgccgccgt
    14281 caaattccca acccacaaaa ccgatttcct tttgttgtga aagggatttg atacggtctt
    14341 ttacgcgcct gatttctgcg ctgttgttgg ataacgccca agtcgcaaaa ggcttgtccc
    14401 ccaaatgcca actgctcgcc atgtctgctt tcagtttttc aagctgttcc ggcgacaaat
    14461 gcgggcagcc gtcaagggtc ttatgcttgc ggtagtaggc gtttacggct ttcatggttt
    14521 cctgcgattt ttcaaggttt tccagtttct tctccaactt ctgtactgcc tgcgggtcgt
    14581 ctgcgctgat accgcccatg ccggtgctgc ggattttatc aagaagtcct tgtatgtcct
    14641 gccattcccg gtaattgctg tcgcgggctg cgttctgctt ttctttcttc cttgtgggga
    14701 agttagagcc gcccgcaatg aggatagagg gaacacgcgc gtcaatctca tagcctttgt
    14761 tcatgttcgc cgccagtttc cgggcgtaag tgtcaagcag gctgtcgatt ttctcatggt
    14821 acatcgggtc tacccgttgt ttctgccttt ctgcaagctg tacggcttca tctacatagt
    14881 gcctgtattc agccgtcgcg ctgccttgct tataatcaga aaagctgttc atatcctttg
    14941 cacgtttggc ggcagcttca ttgattgtgt aataaggtgc ggcgggggct gcctgtcccg
    15001 gctgtgtagt ctgcggtgct tcgctgtttg cgcctgtatc agcttccggc tcggtctgtg
    15061 cttctttttc ctgtaccggg gctgtttctt cgggaagctc cggcgcggct gcttctgctt
    15121 cgggctgtat ggtagcttcc tgtggggtct gtgcctgttc ggtggtctgt tccttatcct
    15181 gtgccttttg gatttctgca aaatgtccgt ctatggtatc aatcagactt gcggcggtgc
    15241 tgcggattgt ttcaagagag cctttcagtt ctgcaagttc ttttccgctg ctccacccgg
    15301 cgatataccc aaaagaatag tcggacgtat caagcccgta atgttgacag accgcatagg
    15361 caacgctttc cgcctgtacc tcgcgggtgc ggcggtcggg acgttccggc gcgtctttgt
    15421 ctatgtcatg gagtgttgca tgggcgattt catggattgc ggtcttgatg ttctgcaatt
    15481 cgtccatacc ctcgttgata gagatacgct tttcctcata attgcagcgt ccttttacgc
    15541 cgccgcttag tgcttcaaat cccatagcaa acggggaagt cctttcaagg gcagcgaaaa
    15601 aatcctgata ctgttccacg tcgcccgtaa gctcctttat cccaaggtcg ggcaactcct
    15661 taccctcggt ctgcgacacg tcaaaaacag ataccacacg aaaggcgggg atttggattt
    15721 ccttttcctc ggtaacggct ttcccgttct tatcgaaaac aggcttttgc gtgtccgggt
    15781 cgattttttc cacctgtttc ttaacggtaa agggcgcggg cgcaagtatc ttgatagctt
    15841 tttctcccgg ctttacatgg cggtcaaatt ccttttccca ttgtttatag ccttttacaa
    15901 ggttgccgcc ctgcatggcg ataaggattg tattgtttag gctgtaatca tggaatttag
    15961 acatagtacg cagatagtcg gcgtaacggt cgctttcata aatccccaaa atgccctgtt
    16021 ccagtcggtc ggtaatctct ttcattttgt cggcgggctt ttcggaagat aggataatcg
    16081 ggagaaccgg gggctgctct gtggcagtgg tcggtatggc tgcggcagcg tcaaggtctg
    16141 cctgttcctg cggctgcggc gttacccggt attcctccgg cacgtcgcgc ccgtcatacc
    16201 actgtgtaaa ggtgttgcgg ttgttgtaga tatatccctg ctcggtaaat ctgcccccgt
    16261 cattgatagc agcgtcccgc ccgtattcct catacatgaa ataatttttt gcttcttccg
    16321 gcagctccaa ctcgtccagt tcttcaatca gataatgccc gtaatcttcc tcggttttta
    16381 cggacggata aatccagtaa cagtcaaggt tttgtgcaag gtttataatg tcctgcaagt
    16441 cccttgtatg gtcgccgtat gtgatagccg cagcaaattt ttcccggtcg tcgtcaaact
    16501 gcattttcaa aagttcgctc aaatagttca gttcgtcaag gctttcaagc tcggtcaatt
    16561 tccctgccag tcctgcaata gaggactggt attctgtgat atgcagttcc ccgtagtttt
    16621 tgccgtctat cccgatacgg tcaaagactt ctttcaaatg ctcggcagtc gtggggaatg
    16681 atacccattc gcccgcaggt ctgccctcgg tgtactttcc aagattagaa agatacccgc
    16741 ttaaaatcgt ttctgccatg cgttcactcc tttcgttttc aatcatgcgg tgcataagtt
    16801 cgtagtcctc tatgctcatg ctcccgtcaa atagataggg gtcgggttct tcgccgtccc
    16861 ggtaggcttt ttcagagaaa ggcagccccg cagcgtgggc ggcggctctt gcttcctcga
    16921 taagctccgg cggcagcctg tcgtcgtggg cttcgataaa ttccccgata ttgctatatc
    16981 cgttttcgta gtggcgacgc accccggcgg tcagttcgtc aaggttcctg tcctcgccaa
    17041 gatagccgga ataatagccg tttaccacaa cggcggcagg gtcagcctgc ttgatttctt
    17101 ccatgcggct tctgtcctcc gggtaaaggt agtcgcccat ttcaaggtgg aaatattcgg
    17161 cgttccaaga acgtcccgtt ttccaaaacg ccacccaagc gataccgtcc cgtaattctt
    17221 cttgatagtc ctttacggtg tcccgtaaac ttgccatgtg taacctcctt tccaaagggc
    17281 agcgcggacg ctgcatgttc cggtttcatc attcagtacc ccgcgcccgc gtttttgcca
    17341 aagattttat agattttttc aagatttcag agggtaaaac cctccacaaa acagagggtt
    17401 tgggacactt cccaacaagc aaaaatcccc gccgttctcg gtagagaaag cagggatttt
    17461 acggaaaagg gtactctttt cctatgcttg ctattcagtt attttttctt cggtagttca
    17521 atgcggtagt tgacatattt cccgcccgta tcagccaata cgatagttcc gtcgtaggtt
    17581 ttgcccgtct ttggggaata gagctttttc acatttacct tgccggattt taagagtgca
    17641 gcggcaatct ttggggtaaa ggttactttt cgttcttcaa agaaacggtc atttttccac
    17701 atggtaaaga tacattcctt attactgcaa tagtagtttt tctttccctc atagacagga
    17761 gaaccgcagc ggggacattt cccaagctcc ggcttttcct ctttgaaacg ccccttgtca
    17821 gcttcggaaa gaaacggata ggtctttacc aattcccgcg ccatatcctc gataccctcc
    17881 ataaatgcgg cgggttctgc cttgcctttt gcaatctgcg tcagattgtt ttcccattct
    17941 gcggtaagct gcgggcttgt caaggtgtcc ggcaggacgc acacaaggtt gataccgtcc
    18001 tttgtgggaa gtagctgctt gccttttcgc tccacaaaac cgccctttac cagtttttca
    18061 atgacggcgg cgcgggtggc gggagttcca agccctttgc gttctgcgtc cgggtcggtg
    18121 tcctcgcttc cggcgcgctc catagcagag agcagcgacg cttcgttgtg aggttttggc
    18181 ggtgttgtgt catgctccgt taccgttgcc gccgaatttt caaaggtctg tccctcggta
    18241 aatggcggca gggtcttttc tgtgtcgctg tctgcgtcgt ccgtttcggg cttgttcttt
    18301 aaggctgccc gatagcggcg gtcaagttct ttccacccgt cggacagtac cctttttccc
    18361 cttgcggtaa aggactgtcc ggcacattca aagattgccg taaccgcttc aaaggtatgc
    18421 ggtgcggcgg tcgccatgag cagacgcgcc ccggcaaggg taaggatatt cctttcgctt
    18481 tccggcagcg cagcaaggtc ggtctttgaa agctccatag tcgggataat ggcatggtgg
    18541 tctgatacct ttttactgtt cagcaccttt gcaagctccg gcgtgaagtc ttttcccgcc
    18601 ataaaagaaa atttcccaca aagcagcttg ataatgcccg ccgctgtgtc gcccatgtcg
    18661 tcggttagaa atgcgctgtc ggttctcgga taagtaagca aacgcttttc atacagggct
    18721 tgtgcaaggt caagggtctg ctttgcggta taaccgaaca ggcggtttgc ttccctctgt
    18781 aaagaagtaa ggtcaaagag cttcggcggg gctgcggtct ttttctcttt cacaagggaa
    18841 gtacagactg tccgcgatgc ttcgcaagtc gtttttaatg cgtcggcttc tgccttatct
    18901 gaaattcttt cacttgcagc ttccgcgccg gataaggcaa ggcgcacatg gtagtatttt
    18961 tctttcttga aagtggtaat cgctgcgtcc cggtcaacaa gcatttttaa ggtcggggtc
    19021 tgtacgcgcc ccacgttcaa ggtatgattg taaaggacag agaaaaggcg ggtggcattg
    19081 ataccaatta accagtcagc ctttgccctg cataatgcgg aagcaaagag cgcgtcgtat
    19141 tcctcgccgt tcttcagacg ggaaaagccc tctttgattg cgctgtcctc catagaggaa
    19201 atccaaaggc ggcgcatagg tttcttgcag cccgccattt cataaacaaa acggaaaatg
    19261 agttcaccct cgcgccctgc gtcgcaggca ttgacgactt cggaaacatc tgcccggtgt
    19321 ataagctcct ttaaggtctt gaattgcttt tccttgtcag ccgcaacggt gtacttccat
    19381 tcctgcggca aaatcggtaa gctgtcatag ctccactttt tatactgttc cccgtaggcg
    19441 gcagcttccg caagccctac caaatgtccc acgcaccaag aaacgatata gccgcttcct
    19501 gtgagaaatc cgtctttctt ttcctttgcc ccaagtacgg cagcgattgt ctgcgctaca
    19561 ctgggctttt ctgcgataac taaaatcaat gataaatcct cctttcgggt aaaagaaaag
    19621 agcaaccgat ttttcgattg ctcctacaag tgattgtata aaattgtgcg atatgaaata
    19681 gtaaaataat ttgacaaacc ggaagttgtt tatttcttct ttttcggttt tggggctggc
    19741 agttcatcat acattgcatg aaacaagcct gtcaaaaatt ccttattatc aacttcatct
    19801 accaacaaca tctcttttgc tccctcatag ggtaattcat acggagctgt cggcatataa
    19861 ctaattgccg attttactgg tttaacaagt aatctatcat catagatacc gcctacaatc
    19921 ttgccacgat aataaataat aaattctcct atcatagctc gataagtaat ttcttctaac
    19981 tcagatagct gccctaaaat aaactctaag tattccttgc ttgatgccat atttccacct
    20041 caaattctta tttgtttaac tctgtcattt cctatggttg gaacaattat atcatattcc
    20101 atatcgcaag tacatctttt tatcgttccg tctgtcgttt ggtttccagt tttctgatac
    20161 agtaccccac acaaggggac tgtcccttgt aaggacagga agtgcaagcc gggggataag
    20221 gaacaggcgg cgcattatca cgccgcccgt tcggtttctg tatcatcatc ttttcaaagg
    20281 gattgtcggt aaacagggtc atagggtttc ttcctccgtt tcttcatctt cgggagtttc
    20341 cccggtatct gtttccggct cgtcctcgtc gtaatcctca aattcaaaat cttcaaggtc
    20401 ggtatcgccc tttacattct gcttcggctt tagaaactta aagtagtaga acgctgcgcc
    20461 gccaccaagc agggcaaaga ggactaaggc aagcactaca ccgggattgc cacctttttc
    20521 tttcggttct tccggctgct ctgttggtgt tggggtgggt tctttgcctg tgcagcctgt
    20581 catactggta acacataccg ggcaggacgt gtttaccttt ccggcttcgc atttttccgt
    20641 acagttacag ctttccggct ttttggcagc ttcgccgttt tcggtcagtg ccataaggtc
    20701 ggcttcgtcc acttgattta ggaaatgtac ggtctgttcc ccctcggcgg cgcggtcaat
    20761 gaggatatag aagtagttgc cgcttttggt ggtaacagtg ataagctgct tattgccgcc
    20821 gtaatcgtca accagtgttg cgtttccctt tggggtaagg ggcggcgttt cttctttttc
    20881 ctccacaatc acgccgctgt cattagttgc gtcctctgcg ggcggctctg tggctgcgcc
    20941 ctgtgcatag gcagggacag aaaaaccgcc cataaggata agggcggcac aaagggcagt
    21001 cagggtcttt ttcagtttat tcttcatctg cattttcctc ctgttcttcg tagtctgcgg
    21061 cttcggtagc gggtgcggcg ttcatgccgg gaataccgcc gccggacagc atagcgttaa
    21121 gttcctgcgg ggtcagacgc atagctcgta caagctgtac gatttcaagg ttttccgctt
    21181 cggttttctg cgcttccagt ccttttaact tgttctgata ctctgtgatt ttctcgcggg
    21241 tctttgcgat ttccttattg atacggtcga ttttattgtt tgccataggt catttctcct
    21301 ttctttttgg ggctgcttta ccagttcatc agcccgtagc ctttgataca ttgatagtta
    21361 aggtcatagc tctttatctt gcaggcgtcg ccggaattgc cctcaacggt atagacgcgg
    21421 ctgccgtccc tgccaagcac aagccccaca tggtcggcaa cgccgtctaa atcccaatcg
    21481 aaaaagatag cgtcgcccgg tgcgatattc tcatagccgc gtgcgcccca ttgtcccctt
    21541 gactgaaacc aagggacacc ctgccattcg cagcccgcaa agcgcggttc gctttttccg
    21601 gcttgattgt agcaccatga tacaaaacag gcgcaccatt ccacgcggct gttaaagccg
    21661 taccaagacc agtaagggta gccgcccaca tttccgactt gctgctttgc aagctccata
    21721 agttcttgat ttccggggcg cgtaccgttt acaaactcca cgccgcttaa atcctcggac
    21781 gggcttccgt cgggagaacc gccgccgaaa atcagcggtt tgttgccgct tgtttccaag
    21841 tagacatgga acatttcaag ctgctgtggc gtaagaagtt cctccgcaag ctcggaaatc
    21901 ggcttgtttg tcagtttcac attcaagatg taatactcat aagggacttc ctcggtggtg
    21961 gtttccccgg tttctgggtc ggtgcttgtt tcggtgcggt aacgtatttc aacctcctcg
    22021 gtcagcgtca aggtgtactg catggcaaag atacgttcaa gctctgcctg tgcgctctgc
    22081 ggggtgtagc tctgcaagag ggcggtcaag taagacgcaa gttcatgtgg gttatgtccg
    22141 atactgtcaa ggtcatagcg gtattcatca tagccgaggt gggtgttttc gatattgtca
    22201 atctcacttt gcagcccgtt ttccaatgca gcatagttgt tttcaactgc caaaaggtcg
    22261 ctgtcctcgg acgtgtagga agtgccaagc acgccattag tcaagcccga aaacatagcc
    22321 ccgcaagagg acagccctgc ggaaatcata ataaagagaa gcagcgcggc aactgcaatc
    22381 aaaatgcccg ccgggtgtct tgccgcaaat gcggcggctt tcctcgtttc ctcggcggtt
    22441 ttcttcgccg tctttcttgt ggcttccgct gcggcttttg cccctccttt ctttgccgcc
    22501 tttgcatact ggcgttttat ctgctgtttc tgcatgaaac gggaaagcgg gttagaggta
    22561 agctgcgggt tgtcgtgcag ggtcttgtga tactggaagt tgacgtttgc cttaaatgct
    22621 gccttttctg ccttcgctgc ttcccggtag ggtttcagct tatggctgcg gtagccctgt
    22681 tttaacttcc gcgccccgta tttcgcgccg cgctcggcaa gttcctccga cttatgcgcc
    22741 ccctcaacgc cggaattgtc tttttcaacc gaatgtatct tgttgtggac gaaaatcccc
    22801 gcttcctgtg cagggcggga cagcgggttt ccctttttgc cgccgggtat cggtttttcc
    22861 tgtttttcaa agagtaagcg ggtctttgct tttcctgtgg cttcatcaaa ggtgcgctcc
    22921 ttaaccagtt tcttttgctt tgggatagct gcctttgccg cgtccagtcg gtcagcggct
    22981 ttgtttgatt tctcaatata gcgttccagt tccggggcgg caagttcttc ctcggtaaac
    23041 tgcaagcggg aagtggaagt acgcaaagca gtagcttcct gctctttttt gactgccttt
    23101 ttgtttgcct ttcgggtatg tgctgcgtca agatgttcta tgacgcgttc cgctgcggca
    23161 ctgtccggct gtgaaaagtc gctgtctgct tcccggctgc tgatacgctc ggcggtctgc
    23221 tgtgtttcgt tgacttccac cacgccgtcg cgggtcattt tctgtgtgat tttatcgcgt
    23281 ggttttaatt gtttgatagt cctgcacctc caatccgcgc cctcgcaagg gcgcaatact
    23341 ctgcatttaa ttcaatcccg atataatggc ggtcaaggct ttttgctgca aatcctgtgg
    23401 ttccgcttcc gaaaaacggg tctatgacaa tcccaccttt gggacagccc gccaagatac
    23461 aggtttccgc taattttggg ggaaaagctg caaaatgcgc ccctttatat gggacagtgt
    23521 taatcagcca cacgtcccgc ttgttccgcg tggtcggcat gagcgcgtcg tcatagtagc
    23581 caccgtcccg cggtcggttt agtccctgca cctttccttg tccgggtatt tcctgtgtgt
    23641 atttgctgtt ggtgctgcgc gctctgcgat accgttctgc ggtagtaggt gctaagggtt
    23701 ccgcaatggc ggcagcgtca tagtaatatt tcttttcctt tgaaagcaga aagatatgtt
    23761 catagcagcg ggtagggcgg tctttgcagc tttccggcat aggattttgt ttttgccata
    23821 taatatcgct ccgtagatac cacccctgtt cacgcaggga aaacgccaag agccagggaa
    23881 taccgattaa gtccttttgt ttgcagccgg taacgcggct gttccttgca atccgctgtc
    23941 cggtgcgtcc ctttgggttt tttgggtcag catagccgcc tttatttcct gtgccgcaat
    24001 aggtgtctgc gatattcagc cataacgtac cgtcctttct aagcacccgg taaagctcgc
    24061 gaaaaacaga ggtcagcctg tcaatatact gttccggcgt atcctcctgt ccgatttgtg
    24121 cgtcaagccc atagtcccgc agcgcaaagt acggcgggct tgtaacgcag caatggacgc
    24181 tttcagaggg cagctctttt aaggcacaaa gcgcgtccct gttgataatc acgtcggttt
    24241 tcataggctg ctcacttcct ccggcttcgt cgtcataatg cggtaaagct cggtgtcttt
    24301 cgggaaatgg tctacaaagg gcagcaccac attcccataa aagataagtc cctcgcctgc
    24361 ttcggtgtgg gttacatact tcatctgttg cggggagata ttaagctgct ttgcaaggat
    24421 agcccggtcg ccctgtgcct gattaagcat gaggacaaaa tcagagtttt caaagatatt
    24481 ttccacctcg cgggaagcaa gcaagtcctt tatgttctgt gtgatagctg tcggtatgcc
    24541 gccccattta cgaaaacgct tccaaatctc cacactataa gcggcggtct gttcctcttt
    24601 taagagcaga tgaaattcgt ccatatagta gcgggtagat ttcttttctg cccggttaat
    24661 ggtaacgcgg ttccatatct ggtcttgcac aatgagcata cctaactttt taagctgttt
    24721 tccaagctgc ttaatatcaa agcaaacaag gcggttattc agttccacgt tggttctgtg
    24781 gttgaatacg ttaaggctac cggaaacata cagctccaat gccgccgcaa tgcgggcagc
    24841 ttccggctcc ggctgtctta gcagctcgtc gtaaaggtcg cccaagatag gcatttttgc
    24901 cgggtcgggg tctgcaagga acgggcggta cacatttctt acagccctgt caataaccgt
    24961 cttatctacg ggctgcaagc cctctttgcc gccaatgaca agctcgcaga gggaaaggat
    25021 aaaatcgctt ttcagtgcaa gggggctgtc gtcctcgctg taattaaggt tcatatccat
    25081 agggttcaca tactggggcg taccgtccat gccttttccg gttggggata agcgtatcac
    25141 ttgcccgcca agccgtttta caagggcaaa atattccgct tccgggtcgc agataaaaat
    25201 atcgtcgtcg gtaatgagaa aggcatttgt gatttcccgc tttgctgaaa aggattttcc
    25261 actgcccggt gttcccaaga taagcccatt cgggttttta agctgcttac ggtcgcagag
    25321 tatcatgttg tttgacaggg catttaagcc ataatacagg gcagccccgg tctgaaatag
    25381 ctcctgcgtg atgaacggga taaaaatagc ggtgctgctc gtcgtcaacc ctctctgaat
    25441 agggataagg ttctcgccca aagggataga ggacattagt cccgcttcct gctgataatc
    25501 aagacgggtc aaggcgcagt tgtatttctg tgcaatgccc gccgccgcga aaatgtcatt
    25561 ttccagtttc cgtttcgtgt ctgccatgtt taccacaagg aacgtaagga gaaacattcg
    25621 ctcgttgcgg ctctgtaaat cctgcaataa attctttgct tcatttccaa aagtggcaag
    25681 gtcggacggg attatatcca tgtcataacc gcttctaact gcttttttct gttcctcaat
    25741 cttcatcttg tcaaggtcag tgattttgcg cttaatggtc ttgattgctt ccgactggtc
    25801 gatactgcgg atatgtaagt tgacgattac gcctgtttcc aaatctaaca tatccgctaa
    25861 gatacggtcg tttagctccg gggcaaagat ttcaaggaac gataccgccc ccgtcttttt
    25921 ccccatagca aagtagcgtc cctccccgaa cttgaaagag gacggggcga taaagtcctt
    25981 tgtagtaagc ccggtcgggg tcagccagtc ataagagaat gagaacggct cgcctgccgg
    26041 gtggaatacc ccatgcagca cctttaagcg ttcatagccg gataaggggt gcgccgatac
    26101 cccaagcacc ttgaagttgt tgagtacgtc cgtttcaata cgggaaaggc gcgcccttgc
    26161 tgccgccgga ttgtccgctt caatggaaaa cgtgatgtac ttgtgcttta caagcccgtt
    26221 gttccctttg gcaagctgcg atttcagcat atcggaatac tcggtgcgga tagaattaaa
    26281 atcgtcattt tggggcggga tatggattgc cctcgctgcc tgttcctgct gtgagccttg
    26341 attgatgaaa gaaagctgca cgttcacgct tgcgtcaaag tagttgagaa aatcgcacca
    26401 gttttcaaaa atggcggtct tatcgtctgc ctgtgcaagc tgatagttga tgtcctcaaa
    26461 agcaatgctc tttgaatact tgtgctgtgc cactttgcag ataccgtccg ggtacatggc
    26521 aaggtaggga atggtctgct gtgcggtgtg tgctttgccg tccccctttg cctgccggat
    26581 aaccgccgct atctgtttct tttccgcgcg ggtcagctta cgcctgtttc ctgcggcttc
    26641 ccgcgtcttt tttgtctgtc cttttgacaa tcgctgatac ctccttttct aatgcccgct
    26701 gtttctccaa gagtgcatat aggttttccg tctgatacgg tcgcactttg ggacgggtaa
    26761 attttgtctg aatgatgttc tttacaacca cttcaagggg ctgtccatgc ttttcataca
    26821 tggcaaccaa gaagcaggga agcataacga caatcattgc cattgccgca aggctcgtcc
    26881 ctgcgctgtc tttgagcaaa aagaaaagcg gcagtcccat aagaagtgcc actgaaaaac
    26941 aaaggatttg ccgctttgtc agattgaaag cgacttttgt tttgactttg gataagtctt
    27001 taggtacagg tacatacgcc aagaaaaacc tcctttctgc ggggcttgcc cgcgtgtgct
    27061 gtgttaatgt gcattaaaaa tagattttgc cagtgcgccg gatttgaaca gggaaaaaca
    27121 gaggataacc gtataggctg caagggaaaa aatcgcgctg tgcaggttat ccgctaagac
    27181 catttcgcct accaaaactg cgtaaatgcc gacgcatatc ataatgagga agccttgaaa
    27241 gcccagtgca aacagcccct ttaggtagtt gttcccaatc tgtccccatt cccggtttgt
    27301 catggtggca aaggggatag gggaaactga acagtaggcg tatatctcaa tcattctgcc
    27361 gtagaggata acggttatca gtacggacat gattttcata caaaggctta caaggctcgt
    27421 ttccatgaca agtaacagta gttcggggat ttccatatct tttagtccct cctgcatggc
    27481 ggcaagggct tcggttacgt cgatattcgt atcgccgccg attacccccg ccgcaccgga
    27541 cacaatatgc tgcgccatat caaagactgc cattgttata tcaaaggtat gcgttaccaa
    27601 gaaaacggca acaaaggctt tgaaaaacca cttaaagaac atgaatgttt ctatgtcgtg
    27661 catattgttt ttttccgtta ccatgctgat aagctcataa cagaggacat aggtaatgac
    27721 aagccccgca atgggtacaa tcacattttc cgacaggttt tgtatcatgc tgaaaatgtt
    27781 tgcgttccac ccctgcgggg tcttgcctac ctccgcggca atggtgccta ctttttcgtt
    27841 tacgtccccg aacatagtgg aaaggttgcc gttaatggct ccgataagga tttcttttat
    27901 ccattcgtta atcgcttcca gtatgctctc cataggctac cctccgtaac tttatccgaa
    27961 cagaccggaa agaagcggta caaggatagt gccgataagg gcaacgccgc cgcccgccat
    28021 gagctgctta atgccttgac ttttcgcacc cggattgtcg tttccatatc cctccaagag
    28081 attgataacg ccccaaatgc ctaagcctgc gcccagtgcg ataacaaggg tctgtaatac
    28141 gtcgatagca gagttaaaaa attccataaa gctcctttct tccgcaaatg cggcttgccc
    28201 tataaaaggg cataaaaaac ggcggtcagt tttgtaactg ccgcggtcat aagcccgccg
    28261 gattgtggcg ggttccccgc gctgcctatg tgtctgccgc gcggcggtat tcagttttga
    28321 gtgtgggcgt tatggatttt ccagtagggg cgcgtattct gtaagttgtc tgtttctttg
    28381 ttactgcgtc aattcctcct ttcttttcag ccgggaacac acatttttcg tcattccaaa
    28441 agatagctcc tgcgctgcat atactcggta acgtaaaggg tctgttcccc tccgcgttcc
    28501 tgcaaatcct cccgcagagc gttttctgca ttttctatca tctttgtaaa ataatgttca
    28561 cgacgtatat catcagtttt tgttttctgt ctgctcatag tgtaacctcc cgtaaaatca
    28621 aagttcccgc cgcacccgtt tctgctcata cagggcttca caccgtttca caaactgccg
    28681 gatagctgcc tgccgttgct gttcccgtcg ctcggcaatg aaaacaagcc cgtttatggc
    28741 ttctgtgatt ttcttgtcct ttttctttct gatacgttta ttcatggtcg gtgtcctcct
    28801 gcaaatcgct ttcgctgatt tcgtagtagt caaatacttc gtccggcttc acaagggcgg
    28861 gtctgcgcct taaatgcttc tccatatcaa aggtattttt cttgtcatag tcagacaggt
    28921 atttatagtt ggggtgcttt gttatgtcgt acttatcaga gaaaaacggg cgcacccctc
    28981 gtagctgtaa aatacatttc ccgccgtcca tgaccgcaat ttcatcttcc gtcataagct
    29041 gcttacctaa tttttgatag ttcagcccat gcgatacctc ccgcccccgg ttctcggaag
    29101 tgttgaagct gtcaatggtt tctttcccca agatttcgga catttctttg agggtggttt
    29161 tctccttgcc gcccaagaaa agagtggtgt cgcagttgcc gacgatagta tcagcgttat
    29221 ctttgtatat cgcctttagc tgcgactggc tctgcaaaat gatggaagca gagatttccc
    29281 ggcttcggat agtggctatg agcttttcaa acttcggtat ctgcccgata tttgcaaact
    29341 cgtctaacag acagcgcaca tggacgggca gccgcccgcc gtattcatca tctgccttgt
    29401 cgcaaaggag attgaaaagc tgcgtgtaaa ggattgatac tacaaagtta aaggtatcgt
    29461 cggtatcgct gataatgaca aacagggcgg tcttgcggtc gcctatggtg tcaagctcca
    29521 tttcgtcggt ttccataagg tcgcgcagct ctttaatgtc aaagggtgca agccgcgcac
    29581 cgcaggaaat gaggatagag cttcttgtct ttcccgcaga taacaggaat ttcttatact
    29641 gccggacggc aaagtgttcc gggtcttttt cctccaaccg ttcaaacatg aggtcaacgg
    29701 gggactgaaa ttccgggtcg tcctcgcggg cttcactggc atttatcatt tcaagcagcg
    29761 tcgtgaagtt cttttcttcc tccggggctt cgtaccaaat gtagccgata agggcgcagt
    29821 aaaagagccg ttccgatttt acccaaaaat cttccccggc tttttccccg tcccctttgg
    29881 tgttggcaat caaagtgttt accagtttca aaatgtcttt ctcgctgcgg agataggcaa
    29941 agggattgta tttcatgctt tttttgaagt taatcgtgtt cagcactttt atccggtagc
    30001 cgccccgctg taagagcttc ccgcactcga ctaaaaccgt acctttcggg tcggttacaa
    30061 cgtagcttga gtgcatttgc attaaattcg gcttcacaaa aaatctcgtc ttgccgctgc
    30121 cacttccccc gataacaagg atatttttat tccttgcata cttcggctgc ttcgggcggc
    30181 tgttcatgct aagccgttcc gtctgtgtca gcagcacgtt attttgaaat accgggtcgg
    30241 tgtagggctt aatatcttcg gcagtccccc aacgggcaga gccgtattcc acacctttgc
    30301 gatatttctt tgcgttcttg cctttgacat agacaatcag ccggataagt accgcgccta
    30361 aaattcctat cagtaaatcc ataggggcaa ggctcggaag cgggtttgca aaggcagcgg
    30421 aaaagccctc cccaagcgac agcaccttgc cggacaggtc agcccccggc gcaaggtgta
    30481 ccgcctgccc gactttatca aagagataca caaagagcag atagggaatg ttgggaagca
    30541 gcagcttttt ccagttgata gcgttcatct tgcaatcccc ctgtctttgt tttttacttt
    30601 gtcccgctgt gcgttcagag ccgccgcctt ttctttgagg gaaacaatca gtttgcggat
    30661 agagggattt tgttcctgcg tcagcttctt tgcggaaaac tccttgaaag ctgcggtcag
    30721 tgcgtccgcg tcccgtccct tgaaaaacac caaatagcgg ggcggggttt cggtgctgtc
    30781 tttctttaag gcaaagtcaa tcccgtattt tttcgccgta ctctcaaagg ctttgatatt
    30841 gtcctttgtg atttcaatgt tggaaacgcc cgcgttctgc ttcataagct gctttagagt
    30901 ctgctttcca tgcgggattt tctgtttgtc atgctgcttt ttcatctgtg caagcagggc
    30961 tttcatagcc ttttggagct gctgcgccgt cagctttccg gtcttgatgt aaagggctat
    31021 ggtcttttcg ttggtttcct cctgcaatgg gtttcctcct ttcgtgttct atggtggtat
    31081 gcccgccgcc gggaagtggg gcgggcatga aaaaaagagc gtccgttttc acgacgccct
    31141 gttaaatccg tacccgcagc ccggaaaggt ctgccgcccg aatacccaca agataccagt
    31201 tgtccccata ctccatacgg gtaggcttcc atttgccccg cacaaagacc tccatgcagt
    31261 cgccgcaatg caagcccccg taatagtcgt ttacgtcaaa gcgaatgtcg tagcggtcag
    31321 cggtttcatc aaaaatcaaa gctcctgttt tctgtgtcat tataaaatcc tcctttgggt
    31381 tggtttacag gctttcgccc tcgtcgcagg tgtaatcggg tatgccggat tttttcggtt
    31441 tcccataaaa tgtgccgtct gccatatcgt gagatacaag ggacgtgtaa tagctgccaa
    31501 tcgtagacgg ggcattgaaa agggcagctt tcatgtactg cttgatgttg cggattttgg
    31561 tggtgttttc ctgcatacag tccagtacaa agcggatatg ttcgctgttc agtttcataa
    31621 attttgcttt tacaagctct gccgggtggt cgtccccggc gatacggatt gttcttcgtt
    31681 tggtgcagac ggtttcaaga ataagggcaa ggatttcctc tatcctgtcc ctgtcaaggt
    31741 atctgtcctg tatcagatag tcgtactcga tattgtcctt gatgatttcc tcatacacgc
    31801 tatatgcgtc tgtcgtttcc tttcgcttcc gttccggcgg ctgtgccgcg tcctctaagg
    31861 gagagggatt tagggaatgg aaaggaatgg aatgggtact tgatatatct gtatttaatt
    31921 tttctttgtt tggtaagtta gtctttaata gttctttatt taattgcgtt ggattttccg
    31981 acgtcgggtt atccgctgtt ggattttcca atatcggaca atccaatgcc gggggctgtg
    32041 gctgttcgta aatggtgtac tcgatagcgg tcattttgcc tttctcgtcg cgtccctgcc
    32101 gccttgtgat atatccggct ttttcaagct cccatacagc ggtgcggata gcgtcgatac
    32161 tttcccggtt gataagggac agccctgcaa gggtgtagtc ccaatcttcg ggaagtgaga
    32221 gcatttgcga taacaagccc tttgccttta gggaaagttc cttgttgcgt aaatggtggt
    32281 tgctcataac cgtataacct ttgttctttt ccacgcgaaa aactgccata gcttcgtcac
    32341 tccttttcgt tggatttgtt acgcagtaga aacgcggttt ggagggaaaa ggtatcaacc
    32401 cttgtctttc ttatctccgc gctctaagag ccacataaat caagacttct tttgccctta
    32461 ctgcgtaaca tgagggcata aaaaaagcag cgttcctgtt ctcctgtgta ggagtaagaa
    32521 acgccgcctt ataggtggtg cttattttat tttttcttat cgacgtgccg cgcaaggtgg
    32581 tgtagcggtc tatccggcag cttcgcaggt gcttttgcat atcaaggcgc aatccgttaa
    32641 aagtagtaaa tgagtagtaa aatcaatctg caaacctgcg aatatgcccg taaatcaagc
    32701 atttttcaag acaatcaact aatttattaa aaatgtaata aaatttaatg gagaaatttt
    32761 ataggtagat aaattttaat atttataaga ttatgttaag gaaacactta aaaattagat
    32821 taaaattgtg ggtatttaaa tcaactaaaa gatattgact atttagtcaa tatctttttt
    32881 tattaaaaag gaatatacat ataaataggt tagattaaaa attgggataa atatgattta
    32941 tatagcatca tttaattaaa aggagctata ttagtattca ttcatacaat tggataatat
    33001 acaatgtcag ccttgcttta tggtgtttgt aataaaccta tttcaatttc tattgtaact
    33061 aatatgcaag aatttaagat aggagtatct atggattatg gtgtattagt aattggtata
    33121 tgctatatag cattaggaat aatttttaaa ttagatagaa aaagatttac ataagataac
    33181 aaaggctaat ttataaattg gtgcttagaa aaccaaatca ctttattagc tagagatgaa
    33241 tttggagggt gtcactttgg tgttttatgt aaagtttatg gaagtaaaac attagataat
    33301 cgtgagtatg atttctataa atcatatata ctattggaat ccaagaaaag gtattggcca
    33361 aatagctaat atctttttaa tttttttgac tctaatatag cattaactat tgcatgagta
    33421 tctttaaatt tatagttaat gtatcttaat tgtttattat aaaatctagg aaggctaaag
    33481 cataaaacac ttaaaaagag aagatgtaca tgagaaatat aattaactat gatgaagact
    33541 tctaaaaatt atttagtgaa aaggaactta gagtgtataa taaaagttcc ttttcactaa
    33601 ggaaaacgat tgttattttc tgaccttcat taccctgata ttatttaaat ataaacaaca
    33661 agaaaattaa caagaagcaa attcaaccta aaatttaaaa tatcgggagg tacaatatgt
    33721 taataaacat gaaggaaatg ctaaaagtgg cacaagaaaa tcaatttgct gtaccagcat
    33781 ttaatatagg aagtgggcaa atacttaaag cagtagtaca gagtgctaac gaaaaaaatg
    33841 caccagtaat acttgcaata cacccaaatg agctaagttt tctaggagat agctttgtag
    33901 ctagttgcat agaagaagca aataagagta aagtacctat ggtaatacac ttagatcatg
    33961 gggaaaataa agaacaaata ttaagggcaa taagatgtgg atttacatca gtaatgattg
    34021 atggctcaca tttaccatat gaagagaatg tagctatatc tagggaaata gtagaaatag
    34081 caaaaggatt aaatgtgtcc gtagaaggag aacttggaac aataggaaca acaggaacat
    34141 caagtgaagg tggtactgat gaaataatat atacagattc aaaattagct aaggattttg
    34201 ttgaaaaaac aggagtagac acactagcta ttgcaattgg tacagcacat ggtatatatc
    34261 caaaaggttt taaaccagag cttaaactag atttattaaa agaaataaga gaagtagtag
    34321 atataccttt agttcttcat ggtggttcat caaatccaga tgaagaaata gcacaagcag
    34381 ttaaactagg agtttgtaaa ataaatattt cgagtgatgt taagtcggca tattataaga
    34441 agtgcagaga actattagaa caaaacccaa gcttatatga gccagatact atatatccac
    34501 catgtataaa agctgcaaga gaagttatag agtttaaaat gaatctattc aatgctatag
    34561 ataagttaaa gtgcttttat aaaaataaaa attaattatg aggtaacaaa ttaaaaggag
    34621 gggagaattt atgataggta aaatactacg aagcgattgt attgtactag ataaacaatt
    34681 acaaacaaaa aaagatgtaa ttaattatat ggcaaatcat ttgtaccatg gaaattatat
    34741 aaataataaa gaaaagttta tagctgatat taataaacgt gaaaaagaat catcaactaa
    34801 tatgggtaat ttaatagcta tacctcatgc taagtcaaat gtagtaaatc aacctgtaat
    34861 gatgtattta agaacagata gagagattac agataatgat ggtcaaaaac ttaggtcatt
    34921 ttttatgata gcagttccag aatcaggttc agatgaacat cttaagatta tatctaaact
    34981 agctacatat ctaatggaag aaaattttct taagcatcta ctaagtgcag aaactccata
    35041 cgaaatatta agtatattta ataataaagc tgcagaatca ggagatttta gtaataatca
    35101 aaataaaggt cttatagttg ggataacagg atgtccaaca ggtatagctc atacttttat
    35161 ggcaaaaaaa gcattagagg aagcagggca agaacttgga tatgaagtta aaatagaaac
    35221 tcatggagct ataggtgtag aaaataagtt gactaaagaa gatataaaaa gagctgaata
    35281 cgtaataata gcttgtgata aagaagttca acttagtaga tttataggaa agaaagtgct
    35341 acaaaagaaa gtagtagatg ctaagaatgt ttcattagct aaaaatttaa ttataaacgc
    35401 tgaaaatgga atggctaaag aaattaaacc aggtggaaag accttagaag attcaaaaga
    35461 aatgcttcaa gggtcagaga aaaagggtgt ttataagcac ttaatgagtg gtgtatctga
    35521 aatgattcca tttgtagtga taggtggaat tggtatagcc atagcattta tgtttggtat
    35581 atttgcatca gacccaactc atgaaacata caaccctata gctggattct ttggacaact
    35641 tggaggcgat tcagcattta agttatttat accgatactt gctggtttta tagctaaatc
    35701 aatagcagga agacaaggat ttgctcctgc tatgatagct ggttttatgg cagttattgg
    35761 aggttcaggg tttataggtg gtatggtagc tggttttgca acaggatata tagctttagg
    35821 agtaatgaag ataacagaaa atttaccaga tgtattaaaa ggagtaaatg ctgtattgat
    35881 atgtccactt ttgattgcat ttacgagtgg agctataatg ttctttgtag ttaatacgcc
    35941 agcatcatgg ttaaatgaag ctttacaatc ttggttacaa agtatgtctg gaactaataa
    36001 agtattatta ggtgcaatat tagcagggat gatggcatca gatatgggag gacctattaa
    36061 taaaacagct tcagcttttg gattggctat gttttcaagt aatatatttg aaccatcagc
    36121 agcattaatg gtaggaggaa tggtacctcc attagcgata tcattggcta tgttaatatt
    36181 caaaaataag tttactaaag aagaaagaaa gtcatctcta ccaactgcta taatgggggc
    36241 atctttcata acagaaggcg caataccgtt tgcagctgca gacccattaa gaatgattcc
    36301 agcaaatata attggttctt gtataggtgg ggcaatatgt atgagtttag atataacttt
    36361 aatggcacca catgggggaa tatttgtaat accatttgca tgtagtgcac cagttgcata
    36421 tatagcttgt atattgattg gtacagtaat aacagcattg attatagggg taacaaagcc
    36481 tactataagg agtgaaaata gtgaagttga attacttggt gcttaaatta taaataatag
    36541 taaaaataat taatggaata acaacaagta tatagaataa taataaggat atttagaatc
    36601 agttaactac ttctaaatat ccttaatttt tattttgaaa ttatattatc tattatatca
    36661 atttagtaag gtgtatcata aattctttta tgatacagaa gtatcttagt aattttacat
    36721 agttagtaaa tatgttaaaa tagtacttaa tataaacata gaaatatata aacataggta
    36781 gggagggcta agtatgcttg tgtcgaggca aattgaattt cttaaattga tactaaacga
    36841 aaataaatat agacctataa aatactttaa agataaatta aatgtatctg ataaaacttt
    36901 acaaaaagat ttgaagatga ttgagaaata tttagaaact tttaatataa aaattgatat
    36961 aaaaagaggc tatggaatat taatagaaga tttagcaaag aagaacatag acttaattaa
    37021 tagtttaaat atacaatcga aggaaaataa atctttatca gtagagcaga gaagaatgga
    37081 gattttaaag tatttattgc taaattcaaa taatataact tcaataaatc agttagcaga
    37141 taaatattat gtaagtaaaa cttctatagt aaatgatttt aaatatatag aggcatggat
    37201 aaaagaatac aatttaaaac ttaataaaac tttagaagga actaagatta taggtaaaga
    37261 agtggatata aggaaatcga ttgccaaaat gatggatgat ttattagatg aaaattatga
    37321 acaacatgat atatctgagt taacaagatt agattctgct acatttagtg cattgataaa
    37381 tttatttgat attgattcta ttatatttgt agaaactata attacagaat tagaatacaa
    37441 tttaggatat actataaatc agccctacta tataaattta attacccata tacttatatg
    37501 tttaaagaga atcgaagaag gtaatcaaat agaatctcag gaagaaagag aaatttctgt
    37561 agataatttg gacgagttag tatataaaaa tgtcactaca cttatagata aaatagaaaa
    37621 tagatacaga gtaaaaataa cgaaagatga acttatgtac atatatattt ttttggtatc
    37681 atctggattt agtagcgaaa ataatagtag ttacaatgat gaatcagata gattaagtga
    37741 aagtgaaaaa gtttcatcta taatgataga gaatatgtca cgatttttaa atataaatct
    37801 caaaaatgat gacttactac agaaaagtct agcatctcat gtaaggccta tgttaaacag
    37861 attaagatat gatatccaaa taaaaaaccc gttgttagga gagatacaag agcgattttc
    37921 agatgtgctg ggattatgta tgatgactat aaatattatg acagagtatg ataatctgaa
    37981 gaatattagt atagatgaag tctcgtatct tgccacatat tttcaagcgg caatagaaag
    38041 aaatatgagt agtaaaagag taatagtagt ttgtcataca ggatatggaa catctcagct
    38101 ccttgcagcc agattaaaaa gagaatttcc tgaatggact atagttgata tagttcctat
    38161 gcatcagtta agtaagcgaa atttagatga tgtagacttt gtaatatcta ctgtaaactt
    38221 agatttgaaa gataaattac atatagttgt atcagtacta ttaatggaaa atgatattaa
    38281 taatataaaa aatgctttac tttctaaacc taaaaaaagt atgaacttag atacaaactt
    38341 attaggaatg tatttagaag ataatatata ttttaatttt gatgtagctc agatggaaag
    38401 tttaataggt gtgaatttag ggagtaaata tgaatctatt tcattaggaa aagatttaaa
    38461 tatatatata cataagcaaa gtaaattaaa taaagggata atgaatatca ataatgttac
    38521 tagaacgatt gatatttatt tagaaattag taatcaaagt tttttaaaga atattttaat
    38581 ggaaatatca aatttatata ggcaaaagaa ctatataaac tatataatag attgcaagag
    38641 caaagaggat attaaaatat tatttaagaa taatctattg gagaaaacaa atggaaatta
    38701 gagaaatatt aacagaagaa agcattattt tagatttgaa atcaactaca aaagaaggca
    38761 taatacagga attagtagat aaattatacg atacaggaag tataacttta aaaagtaagt
    38821 ttataaaggc attaaaacat agagaaaaaa taggctctac tagtataggt tatcaaatgg
    38881 ccatgccaca tggaaaaaca aaatatgtaa agaaggcatc catagtaata ggtaaatcta
    38941 aaaaaggtgt agattggcca agtatagatg gcttacctgt aaaggtaata attatgtttg
    39001 ctatgccaca atctggaggc aatgaagata tgatattaat atctaaaata gcttacattt
    39061 taggtggtaa aaatataggt gaaagacttt taaatgcaca tacaaaaaaa gaaatattag
    39121 atatatttta tgaagtgtag gtattgtata aattataaat ttgaggtttt taaaataaaa
    39181 tgtgtattct tttaaatagt ataaattaat ttttagacac tagagaggag aggatatata
    39241 tggcaatata tttagaaatg cctaaattaa tacatgaatt tccttttcgt agtctatcaa
    39301 atgaaggaga agtattaaca acaccacact ggcataagga aatagaaata ttacttataa
    39361 cagaaggtgt agtaaatcta tttataaatg acaagcctat gcaattaaaa aaaggcgaga
    39421 tagtgataat aaaaggagga gatattcatt atatactgcc atcaccggga agtgaaagat
    39481 tagtatttca atttgatata tcttttttta atgatttaat tttattaaat gaggaaaaag
    39541 aaagcttaat taatttattc agtagtataa aaaaatgtag tagagattgg tcagcaaaaa
    39601 taagaaagtc agttttcgat atattgatag atatctacaa tgaagaccta tacaaaatag
    39661 aaggatatag ttatgcaata aaaggtaaaa tgtataattt aattcctata ttatatagac
    39721 agattcctag agaaattgaa gaaaatacag tagaatatga aataaactct aaagagatat
    39781 tagagagatt gaacaatata ttcaaatatg tagaaaaaaa ttataaacaa cctataaaat
    39841 tagaagatgt gtcttatgaa gtaggattta gtgtatatta ttttactaaa tttttcaaaa
    39901 aaaatacagg aaaaacattt ataacttttt taaatgaata tagattagaa aaagcaaagt
    39961 ggatattact taataacaat atatccattg tagatttggt tgaagaagtt ggttttggaa
    40021 gtacaaaaac tttttacaga gtatttaaag aaaaaattgg agtctctcca ttagaattta
    40081 aaaaaagata taatagcttg gagaaaaaag ggagtgtctc aaaatgaact tagttcatga
    40141 ggcactttct tttatatgaa atcaaatata ttttcagtat ttcaacaatg aaaattccat
    40201 tcatttataa agtctaaatt ttagttctca atacaacctc tatttcgaaa taaatccttt
    40261 tatatatata aatatacagc aatatatgag aataagataa caataatgag gatataaaaa
    40321 tgtatttgct atatatataa ttatatacat agatatctaa tattgatatt taatatgaaa
    40381 gtattgaaaa tagacaatat taattatagt gagtaaaaaa tataattgga tttttaaatt
    40441 gaattattta aaaggtatag tatgaaaaaa atcttataac taaatatgag atagaaatta
    40501 taaaacttta tattgtgaaa gaggtagatg catgaaatta gggaaagatt ttttatgggg
    40561 aggggcaatt tcagccaatc aaagtgaagg attttattta gatggaggaa aaggtttatc
    40621 tcagtttgat aaactcccat taaatgataa gagattaaaa gatgtatgtt tagatgagga
    40681 taatatttta aatgaaaata atcaatacta tccaagtcat atagggatta aattttgtga
    40741 aaattacaaa gaagatatag caatgttagc agaaataggt tttaaatgtt ttagattttc
    40801 tatatcttgg tcaagaatat tcccaacagg agaagaaagt gaaccaaatg aaaagggatt
    40861 acaactttat gataatataa taaaagaatt aaaaaaatat aatatagagc cattaataac
    40921 gataagccac tttgatatac cttattctct agttgaaaaa tattctgggt gggctgatag
    40981 aagagtggtt aatttatatg taaaatatgc tgaaattttg atgaaaagat ataaagatga
    41041 tgtcaaatac tggattcctt ttaacgaaat gaatatgata atgcatattc catatatagg
    41101 tgctgggtta acttttatag ctgatgaaaa tcaacttcag aaaaaatatc aagcagcaca
    41161 ccatcaatta ttagctaatg caaaaactat agaaatagga aggtatataa ataaagaatt
    41221 taagtttggg tgtatgttag ctgctggaaa aacatatcca tacacatgta aaccagaaga
    41281 tgtatttgca actttagagc atgaaaggca taatttgttt ttttctgatg ttcaagttag
    41341 gggtgtgtat ccaagcttta tgagttatta tttaaaatca aatcaaataa aattaataat
    41401 aaaagaggaa gatttatcaa cacttaagga taatactgta gattttgtat cttttagcta
    41461 ttattctagt gcttgtagtt cagctagagc tgaaggttta gaaaaatcaa aggcaaacgg
    41521 accagaaacc ataaaaaatc catatcttcc aaaatcaaat agtgtatggc aaaacgaccc
    41581 tgtagggtta agaataacta tgaatcagtt atatgaaagg tatcagttac ctttatttat
    41641 agtagaaaat ggactgggta ctcaagatat tgtaaatgaa gatggaaaaa taattgatga
    41701 ttatcgtata gattacatga aagaacatat taaaaatatg attcttggag taaatgaaga
    41761 tggaatagag cttataggat atcttatgtg gggatgtata gatgtaacca gtgtaagtga
    41821 aggaaagatg acaaaacgat atggcataat tcatgttgat tctgatgact atggaagagg
    41881 ctcatttaaa agaactaaaa aacaaagtgc tttttggtat aaaaaagtaa taaaaacaga
    41941 tggagaaatt ttatttaatg atatggaaac tcatgcataa ttcattttaa gaaaggagtg
    42001 ttattatgag tttaatattt actactaagg accaacttat agagcaaggg tttagtaagg
    42061 atacaataaa atccaatgaa acgctgttta cactagctaa tggacattta ggagttagag
    42121 ggaatattga agagtctgat tttaataatg agtatgtaga taatatgggt acatatgtaa
    42181 atggatttta tgaagaatct ccaattacat atggagaaag cgcttatggc tatgcaaaac
    42241 aaaatcagac tatctgtaag ttacctaatg ctcatatagt aaatttctct attgaaggag
    42301 attattttga tttatcaaaa ggtattacaa gtgaacattc taggatacta gatttaaatg
    42361 aagggattct taaacgttca tttatatggg aaaactctcg tggtagaaaa gttaaagtat
    42421 ctatagaaag attagtatca caagatatag aagaattaat gatgatttca tatacaataa
    42481 caccattgaa ttttaatgga aaaataaaat ttaaaaataa aatggaaaat agtatggaga
    42541 agtttaattg tgaaaaagta gatgacccaa gagtggcatc tataaataag aagcaattta
    42601 aaattaatgt aaaaaaatat aacaataagt attttatgaa tttagttaca gaaagaagta
    42661 gattagggtt atggtgtggg tttgattcag atattacttg tggaaatatt acaaatgaga
    42721 cctataatgt ttcagatgaa tgtattgagc gagaaataga agtctatgcc aaaaaagatg
    42781 aaaggttaaa tttagaaatt gttgtaggct atagtagagt atattctgat ttaaatctaa
    42841 atgaagaacg tttaaaatca ttagaatgtc tggagtcgtt aaaatgttta gaatcagaag
    42901 cacatttaaa atcattagaa ttggttttaa gagaagcatg taatattgga tttgataaag
    42961 tcaaaaatat tcagagaaag aaaatgaatg agttttggac aacctctgat gtagagattg
    43021 aaggcgataa aactcttcaa ctggggataa aaaccaattt atttcatatt tatcaatcag
    43081 caggaagaga tggaaagact aatatatcag caaaaggtct tacaggtgat ggctatgagg
    43141 ggcattattt ctgggataca gaaatgtata tattaccatt ttttatatat actcaaccat
    43201 ccattgctag agctttatta gattatagat actcaatctt agatgtatca agagaaagag
    43261 ctagagaact tggaataaat aaaggttgct tatattcatg gagaactata aatggtaatg
    43321 aatgcagtgc atattatcca gcaggaacag cacagtttca tataaatgca gatatagctt
    43381 atggtattag aacgtatttt gaagcaacta atgatgaaga atttatgatg caaaatggct
    43441 tagaaattct tatagaaaca gcaagatttt gggttgaatt tggagattat atagaatcaa
    43501 aaaataataa attctgtata aatggagtaa caggaccaga tgaatataca gcaatagtaa
    43561 acaataatta ctacactaat ttaatggcta agtataattt aatatatgca gtacagatgg
    43621 tagataaatt taagcataat ttattttcaa atgaagtatt tagaaaagtt aaattacaag
    43681 aatatgaagt gaaaaactgg ataaatgccg ctcaaaatat gtatttacca tatgatgaag
    43741 agaagaaaat aagcaaacaa gacgattcat ttttagaaaa atcagtatgg gattttaaaa
    43801 atacaccaaa agaaaaatat ccattacttc tccattatca tccattaaca atatatagat
    43861 atcaagtaaa taaacaagca gatactgtac tttcagcgtt gcttttccct gatgagttta
    43921 gcttagaaca aaagaagaga gattttgaat actatgaaaa aataactact catgattcat
    43981 ctttgtcaag gtcaatattt ggaatgatgg caagtgaaat tggaaatcat gaaaaagctt
    44041 ataactattt tatggacaca gcattaatgg atattaataa cctacaaaaa aatacaagtg
    44101 atggaataca tgctgctaat atgggtggaa catggatgag tttagtatat ggatttgcag
    44161 gaatgaaaat aaaaaatgat aaattaagtt ttgaaccaag gatacctaaa cactggaaaa
    44221 atattaaatt taatatatta tttagagata atttagtttc tgtaaatata gaagaatata
    44281 aatcttatta taagttaatc aaaggtagaa atataaccat attacatgat aataaagaaa
    44341 taaaattatc aaagaacata atagaagtta ataattgttt taaagggagt aattaatatg
    44401 aaagcagtct tatttgatct tgatggagtt ataacagata ctgctaagta tcattttgaa
    44461 gcttggcatg agatagcaaa atcgctagat atagaaatag atgaaacttt taatgaaaaa
    44521 ttaaaaggca taagcaggga agaatcatta gataaaatat tgttatctgc taataaagta
    44581 gatagttata ctaaagagga aaagttaaag gtgtgtgaag aaaaaaataa catttatata
    44641 aagttaataa actcattaac atataaagat atattaccag ggataaatga gcttataaaa
    44701 gaacttaaaa aaaataatgt gaaattggga atagcatctg ctagtaagaa tgcacctagg
    44761 atattagaat ctttgggaat aaaagatgaa tttgattgta tagtaaatcc atctttatta
    44821 aaaaggggaa aaccatatcc tgatattttt attgaaggga gcaagatgct aaatttaaaa
    44881 caatatgatt gtgtaggaat agaagattct tattcgggta ttaaagctat aaatgctgcc
    44941 aatatgacat caattggtgt ggggaaaaaa gaagtactag gaaaatgtga ccatattgta
    45001 gaagatacta gcttattaaa ttacaataca ctattacaag cttggagtaa aaagattaat
    45061 ttggcctaaa actatattaa aaattatttt aaaagagagg ataaggtatg aagaataaat
    45121 atgaagatgt agctcaattg ataattgatg gacttggagg aaaagataat ataattgact
    45181 taactcattg tatgacacga ttaaggttca tcttaaaaga tgaaaagaaa gtcaatgcag
    45241 ataaattaaa atctatagat aaggtagttg gagtaaactc aacttcaact caatatcagg
    45301 tcataatagg taatgaggta ggtgttgtat ttaatgcaat aataaataag gggataaata
    45361 ccacaggaga tattaaaagc gatataaaaa ataaaaataa aaaaaatagt attttaaata
    45421 atataataga tacaataact ggttgtatga cacctatgat accagccctt acagcatctg
    45481 ggatgataaa agttatatta tctctttgca ccacttttaa ttggctatca tcaaattcaa
    45541 gtacatatag agtattagat ataataggag atgtagcatt ttactttatg cctataatac
    45601 ttgcaataaa tgcagctaaa aaatttaatg ttaatacgtc aatagcagtt atagtagtag
    45661 gtgtgttttt acatccaaat ttctctgcat gggtatcaag tggggaccct atatcattta
    45721 taggagtacc aattcaaggt gttatatatg cagcatctgt tatccctgca cttttaacag
    45781 tatggatgat gtcatatata gaaaaattca ttgataaatt aacacctagc atgttaaaaa
    45841 caattttaaa tccaacttta gtattattaa taagtgcgcc aatagcatta atagtaattg
    45901 gaccaatagg aaatttctta ggagagggat tagctagtat aataaattta ctacaaggaa
    45961 ggttaggatt tataatggtt tgtttattag cagctgcaat gccatttatc gttatgacag
    46021 ggatgcatca tgcattgact cctatattca tatcagcatt tgcaactaca ggacaagaat
    46081 cgctaatatt aatatctcaa gtttgtgcta atttagcgca aggtggcgcg acattagctg
    46141 tagcattaag aagcaaagat agtaagatga aacagttagc atcagctgca tggatatcag
    46201 ctattatggg tataactgaa ccagctcttt atggagttac attgaaactt aaaaaacctg
    46261 ctatgttagc atctatagga gcaggaatcg cagggttatt tgcaggaatt actcatgtga
    46321 ctttatatgt tccacaaaat agtataatgg cagtacttgg attatcaggg gaaaagggga
    46381 tttcgaatat agtaaatgga cttatagtta tatttatagc attggttgct ccatttatta
    46441 tgacatatgt ttttggagtt gaaaaaaatg ttaaggaagg tatagataat gtagtagagg
    46501 aagtataata ttaactattt acattaatag aatttttatt attattgact ataacttgtt
    46561 ttgtatttat attgatgcaa agaaataagt taatattaat aagaataatt attagaactg
    46621 acaaaaagca acacaaaaga ttgatgaagt atatggaaat aaaaagttga caaaatatgt
    46681 ctgatgtttt atcatattaa ttggaattag gtagtaattg tttaagtaca agctaagctt
    46741 attcattaat aatagcagta tcctaggaag gagtatgccc attattatgg ataagctttt
    46801 tttatgctta aaaggaggat aatcatgata attaataaga tattaaacaa taatgttgta
    46861 atcacattag atgataatga tgaagaagtt atcgttatgg gtaaaggaat tggttatcaa
    46921 aaatcaaaag gcaatctaat agataaaact aaagtaaata aagtatttag aatatcaaat
    46981 aaagaaatat caaacaaact acaagaatta ctcaataata ttcctataga acacatgaaa
    47041 ttatcaagtg aaattataga atacgcacaa ataaagttaa acaagaagct aaatgaaagt
    47101 atttacatat ccttaagtga ccatacatat tctgcaattc aaaggatgaa agaaggaata
    47161 aatgtaaaaa atgcaatcct ttgggaaatt aaaagatttt ataaagaaga gtttgaaatt
    47221 ggaatgaaag cactagatat tattgaaaac aaaacaggta taaaattacc agaagatgag
    47281 gctggattta tagcctttca tatagtgaat gctcagttaa gtgaaggtca tactttggca
    47341 tctgatatta cgaagctaat acaagaagta cttagcatag ttaggtatca ttttaggata
    47401 gaatttcatg aagaatcagt gttttattat agatttataa tgcatttaaa gttttttgcg
    47461 caaagattgc ttttagatag cgcacatgaa ggtgagacag acaaagaatt actaagcatt
    47521 attaaatcta aatataataa agagtttgaa tgtgtagtta aaattaaaaa tttcattaaa
    47581 aaacaatata actacatatt aacagatgat gagcttatat atttaacaat acatttagct
    47641 aaagtagtta aagactcaaa tatgtaagtg aaataatttt tcagcagtat attaagaatg
    47701 attgaaagga gtgatactaa caaaattata cttatggttg gatggtaaca ttgagttggc
    47761 caaaccttca aatttcaaaa aattaattta aacgaaatat ggaggagaaa aatgggaaaa
    47821 tacaaacaat tagcacaaga gatagtaaac aatgttggtg gaaaagaaaa tattagtgga
    47881 ttagtacact gtattacaag attacgtttt actttaaaag atgaaagtat agcaaatgac
    47941 aatgtattaa aaaacatgga aggtattgtt actgtaatga aaagtggagg acaatatcaa
    48001 gtagttatag gaaatcatgt tgaagctgtc tataaagatg tagttgaaat aattggttta
    48061 gatgattcaa gtacaagttc agaaactaaa aaatcaggta atatactaga taaaggtata
    48121 gatataatat caggaatttt tcagccagtg ttagggataa tggcagcttg tggtatgttg
    48181 aaaggattta atgcattatt tgtagcaatg ggattatata gtgatgtatc tggtggttat
    48241 atggtaataa atgcagcggg agatgcatta tttacattcc ttcctttgtt cttaggatat
    48301 acttcagcta agaagtttgg attaaaacca atgttgggat tagcactagg agctgcagta
    48361 tgttatcctg ctatacaagg tagcagtata tcagcaggag cagatgtgat gtacacttta
    48421 tttaaaggaa caatgtttgc atcaccagta tatattgatt tttttggact accaattgta
    48481 tcaattgatt atactggaac agttgtacca attatattta tagtgtattt tgcttctagg
    48541 tgtgaaaaat tatttaataa gtgtgttcca gatttagtta agtttttctt tgtgccaatg
    48601 cttacattat taataacagt gcctgttgca cttatagtta taggtcctat agcaacattt
    48661 ggttctacac ttatttcaca aattgtaatt tcaataagag attttagtcc actattggca
    48721 ggtgctatag taggatttac ttggcaaatt cttgttattt ttggtatgca ctggggattt
    48781 attcctgtat atataaacaa tgttatgact ttaggatatg ataatgttat gatgccattc
    48841 tttgcctgta catttgcaag ttcggcagtt gtgttggcaa tatttttcaa gactaaagat
    48901 aaaaaactaa aagagatggc aataccaaac tttatatctg gtatatttgg agttacagag
    48961 ccagcaattt atggaatatt attgccttta aagaaaccat ttataataag ttgtattgct
    49021 agtggtattg gaggcgcttt ctatggattc tttaatctta gaaaatttat aactggagga
    49081 atgggaatat ttgaacttcc agcaatgatt gaaccagatg ggggaatggg taacttaata
    49141 gtagctcttt ctggtatagc tatatcaatg gtcgtagctt ttgtattaac tatgatttta
    49201 tataaagatg agaaagtaga agagtctaaa aaagtaagaa ataagggtaa agaagaaata
    49261 aaagaagtca agtctactaa attagaaaga gaaatagtaa caagtcctat aaaaggtgaa
    49321 gtattaaaat taagtgatat agaggatgca gcatttgcat ctggtgtact aggtcaaggg
    49381 gtggcaataa tacctagtga tggaaaagta gttgctccag tagatggggt agttacaacc
    49441 ttatttccta gtctacatgc tatagggatt ctttctgata caggtgttga agtgcttatt
    49501 catattggat taaatactat tcaattagag ggaagaggtt ttgaggcatg tataaaacaa
    49561 ggtgatagaa ttacaaaagg tcaaactata ttaaactttg atgttgatgc aataaaagag
    49621 ttggggtata gtactgtaac tccaattgtt attacaaatt catcacaatt tttagatgta
    49681 gtagaaacag aaagtaaaaa tattgaatta gaagacaatc taattacagt cttattttag
    49741 taaagattga ggtattagca taatctagtg ctttggttat aattttagga ggctttgact
    49801 atgagtttta agaatgattt tttatgggga ggagctactg cggccaatca atgtgaaggt
    49861 ggatacaatg aagacgaaag aggattggca aatgtagatg tatgcccaac aggaaaagat
    49921 agaacagcag ttattacagg gaaattaaaa atgtttgatt ttgatgatga gcattattat
    49981 ccagctaaaa ctggtattga tatgtatcat aattataaag aagatataaa gttatttgct
    50041 gaaatgggat ttaaagtata tagaatgagc attgcatgga gtcgaatatt tccaaaaggt
    50101 gatgaagaaa caccaaatga aaagggactt caattttatg aaaatatatt taaggagtgt
    50161 aaaaaatata aaatagagcc tttagtcact attacacact ttgattgtcc aatgcattta
    50221 gttaaaaagt atggtgcatg gcgtaatcga aaaatgatag gtttctatga gaaattatgc
    50281 aatgttatct ttagaagata taaaggatta gtgaagtact ggctaacttt caatgaaata
    50341 aatatgattt tacatgctcc atttatgggt gctggtattt gctttgaaga aggtgaagat
    50401 gttgaaaaga taaaatatca agctgcccat catgagcttg tagctagtgc aattgcaact
    50461 aaaatagcac atgaagttga ttcaaataac ttaattggat gtatgtttgc agctggaagt
    50521 gtatatcctt atagttgtaa tcctaatgat gtatgggaag ctacaaagtt agatagagaa
    50581 aattacttct ttgtggatgt acaatctaaa ggtaaatatc ctaactatgc gttaaagtat
    50641 atggagcaaa aaggtattac accagaaatg gaacctggtg atattgaatt attgaataaa
    50701 tatacagtag attttatttc attttcatat tacaatagcc gttgtgttag aaccgatgaa
    50761 aatgcagatg atatggctga aggaaacata tttacatctg ctaaaaatcc atatctttca
    50821 tacagtcaat ggggatggcc aattgaccca ttaggtttga gaattacact aaatcatgta
    50881 tatgatagat atgaaaagcc actatttatt gtagaaaatg gattaggtgc taaagatata
    50941 gctgatgaaa atggttatgt agaagatgat tacagaattg attatataag agaacatata
    51001 aaagctatgc atgatgcagt ttctatagat ggggtcgatt tattaggtta tactacatgg
    51061 gcacctattg atttagtaag tgctggtaca ggtgaaatgg aaaagcgtta tggatttata
    51121 tatgtagata gagacaatag tggtaatgga agtctaagaa gaatgaaaaa gaaaagtttt
    51181 gaatggtata agaaagtaat tgcttcaaat ggtgaagatt tatattagac aagaaatcta
    51241 tgttaaataa gaaatttgta ttaaataaga agagtataaa aattaataat aagtataatt
    51301 tttaactttt ttaactatag aaaatcaata cagaattatt ttacattgta tttatgcaat
    51361 agtatgattc acaaataaaa ttttagaaaa taaaattgtt aagaagtatg ggaagtaagt
    51421 gcaatttact ttgtaataaa agatgaaaaa ggcaagctta ggttttaagc ttgtcttttt
    51481 tattatgtat aaactatgat actttaaaat tctatgaa
//
LOCUS       DAFKMX010000003        50723 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFKMX010000003 DAFKMX010000000
VERSION     DAFKMX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.003"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            61..1056
                     /gene="ilvC"
                     /locus_tag="LZB08_000116"
     CDS             61..1056
                     /gene="ilvC"
                     /locus_tag="LZB08_000116"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBM7313637.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1109..2797
                     /gene="ilvB"
                     /locus_tag="LZB08_000117"
     CDS             1109..2797
                     /gene="ilvB"
                     /locus_tag="LZB08_000117"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBM7313638.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVASMEELGKALDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(3158..4288)
                     /locus_tag="LZB08_000118"
     CDS             complement(3158..4288)
                     /locus_tag="LZB08_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7313639.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(4528..5034)
                     /locus_tag="LZB08_000119"
     CDS             complement(4528..5034)
                     /locus_tag="LZB08_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBM7313640.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(5110..5658)
                     /locus_tag="LZB08_000120"
     CDS             complement(5110..5658)
                     /locus_tag="LZB08_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313641.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            complement(5670..6011)
                     /locus_tag="LZB08_000121"
     CDS             complement(5670..6011)
                     /locus_tag="LZB08_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBM7313642.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            6188..6391
                     /locus_tag="LZB08_000122"
     CDS             6188..6391
                     /locus_tag="LZB08_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313643.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            6439..7248
                     /locus_tag="LZB08_000123"
     CDS             6439..7248
                     /locus_tag="LZB08_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBM7313644.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQPKLPTTYKEALQHLIEQVEVNEKLQLESKMKEKVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            7295..7504
                     /locus_tag="LZB08_000124"
     CDS             7295..7504
                     /locus_tag="LZB08_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313645.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(7514..7888)
                     /locus_tag="LZB08_000125"
     CDS             complement(7514..7888)
                     /locus_tag="LZB08_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313646.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            7964..8161
                     /locus_tag="LZB08_000126"
     CDS             7964..8161
                     /locus_tag="LZB08_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313647.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            8192..8584
                     /locus_tag="LZB08_000127"
     CDS             8192..8584
                     /locus_tag="LZB08_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313648.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            8565..8714
                     /locus_tag="LZB08_000128"
     CDS             8565..8714
                     /locus_tag="LZB08_000128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313649.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            8785..9210
                     /locus_tag="LZB08_000129"
     CDS             8785..9210
                     /locus_tag="LZB08_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313650.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTKAAQV
                     SKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            9222..10388
                     /locus_tag="LZB08_000130"
     CDS             9222..10388
                     /locus_tag="LZB08_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBM7313651.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            10403..10738
                     /locus_tag="LZB08_000131"
     CDS             10403..10738
                     /locus_tag="LZB08_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313652.1"
                     /translation="MALDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            10791..11363
                     /locus_tag="LZB08_000132"
     CDS             10791..11363
                     /locus_tag="LZB08_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBM7313653.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            11386..11958
                     /locus_tag="LZB08_000133"
     CDS             11386..11958
                     /locus_tag="LZB08_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBM7313654.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            11958..13916
                     /locus_tag="LZB08_000134"
     CDS             11958..13916
                     /locus_tag="LZB08_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBM7313655.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            13928..16345
                     /locus_tag="LZB08_000135"
     CDS             13928..16345
                     /locus_tag="LZB08_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313656.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYARTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKINKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            16627..16953
                     /locus_tag="LZB08_000136"
     CDS             16627..16953
                     /locus_tag="LZB08_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBM7313657.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKPRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            16902..18272
                     /locus_tag="LZB08_000137"
     CDS             16902..18272
                     /locus_tag="LZB08_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7313658.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVTANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            18296..18433
                     /locus_tag="LZB08_000138"
     CDS             18296..18433
                     /locus_tag="LZB08_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313659.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            18426..18905
                     /locus_tag="LZB08_000139"
     CDS             18426..18905
                     /locus_tag="LZB08_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313660.1"
                     /translation="MDKESIKEIVRELRKEEKENKKREVFHNTKLLLKHYNDLKSHIA
                     NSISDIEDVKSDYDDLLCLDIEDLYILSIKRSKAKTLIMVAHIDMALETLKVTQNKLQ
                     SLEKYKALELYFFKEKTYEDIAELLHCGVATVRRWVNEMVKELGIYLFGVDGLKIKL"
     gene            19044..19301
                     /locus_tag="LZB08_000140"
     CDS             19044..19301
                     /locus_tag="LZB08_000140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313661.1"
                     /translation="MDMINLEEIFCKAKLLDKKYISLKINITEYPSEEIIIIQNTNFN
                     SKLRYYKEIYDDKLNHKYVDGIKIINSTYANGFKEIELSLE"
     gene            19489..20532
                     /locus_tag="LZB08_000141"
     CDS             19489..20532
                     /locus_tag="LZB08_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7313662.1"
                     /translation="MKKKNILNLIKYHAEKNDAGFRTEAYEIARYFDGIGNYQLSEYI
                     MALLSDANTFSPQISEENEVFFKRVEITNEPLPLPETIKDNIIGIVNAVGHNAGVNKF
                     LFEGPPGTGKTESVKQIARILDRELFVVEFDAIVDSKLGQTSKNIALLFEEIRNLSNP
                     SKVIILFDEIDAIAIDRINSNDLREMGRATSSVLKGLDGLNDSVILIATTNLFNSFDK
                     ALIRRFDSVIDFSQYTKEDLIDIGEILLNDFFNKFKSAGRNMRLFKKILRLLDPIPYP
                     GELKNLIKSSLAFSDPNNEYDYLKRLYKTVTKDIQENNLKEIQEKGFTMRELEILTGI
                     SKSQISRELKGDN"
     gene            20533..22755
                     /locus_tag="LZB08_000142"
     CDS             20533..22755
                     /locus_tag="LZB08_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBM7313663.1"
                     /translation="MNNLLQLKGTFEQKSFEGNIGSPKLPSNAIVKTEHLKKLKDDLV
                     KLASFWEKENFFSGALVSVYYNKIAAKSNRIRGLLSKKVKANSTIVGARFTTEGEKKH
                     IITHYIQLDVLYESIRRLDTCINILIKNFDGAITTKINADINSKKIKFEPKLISKTNF
                     LQIIVDCYFVEKFDVFVDEEEFEENAIITIFKTDNKAPELLERIGIELPHTSVIDDTT
                     ILMKPDDLSLLKQKAPYLISMAVSDLAQLDSTYFDFGEKNTRSIPKPTNEPVIGVIDT
                     LFDKNVYFSEWVEYHDMIPDEIPKQLSDYRHGTAVSSIIVDGPSSNPKMDDGCGRFRV
                     RHFGVATDKQFSSFSILRHIDEIVTKNRDIKVWNLSLGSKLEINKNFISPEAAILDRI
                     QYENDIIFVIAGTNKDINNPQIMRIGAPADSINSLVINSVSSDNKPASYSRCGPVLSF
                     FTKPDISYYGGEKQSPMHVCTPMGEGFVTGTSFAAPWVARKMSYLINILGLSREVAKA
                     LIIHASTGWSKQDIAPTLIGHGIVPIRIEDIIKSPDDEIQFILSGVSEKYNTYNYNIP
                     VPINKDKHPFIAKATLCYFPYCSRNQGVDYTNTELDISFGRLNEKGIKPIDNNYQSSG
                     YEHFILEEEARNNFRKWDNIKHIREVYKKGNKDRKAYDKGIWGVSLKTKERLEEKYGE
                     GIKFGIIVSLKEIHGINRIEEFINQCLLRGWLVNKIDVQNRIDIYNIAEETINFDDLN
                     "
     gene            22913..23596
                     /locus_tag="LZB08_000143"
     CDS             22913..23596
                     /locus_tag="LZB08_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313664.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKGKEETVKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            23574..24827
                     /locus_tag="LZB08_000144"
     CDS             23574..24827
                     /locus_tag="LZB08_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBM7313665.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            24833..26272
                     /locus_tag="LZB08_000145"
     CDS             24833..26272
                     /locus_tag="LZB08_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBM7313666.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDVQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            26262..27755
                     /locus_tag="LZB08_000146"
     CDS             26262..27755
                     /locus_tag="LZB08_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBM7313667.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSIKYYLELEKQYKLAMSNIERDI
                     LIWYNRFTENEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKVISKPWTSDGLNFSERIWGKHRPALVNELHTKLTQSIIRGENPKKLV
                     NDFAKRFKVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            27721..27963
                     /locus_tag="LZB08_000147"
     CDS             27721..27963
                     /locus_tag="LZB08_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313668.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            28273..28836
                     /locus_tag="LZB08_000148"
     CDS             28273..28836
                     /locus_tag="LZB08_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBM7313669.1"
                     /translation="MDWLEELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            28848..29738
                     /locus_tag="LZB08_000149"
     CDS             28848..29738
                     /locus_tag="LZB08_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBM7313670.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            29757..29990
                     /locus_tag="LZB08_000150"
     CDS             29757..29990
                     /locus_tag="LZB08_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313671.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            30000..30401
                     /locus_tag="LZB08_000151"
     CDS             30000..30401
                     /locus_tag="LZB08_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313672.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            30395..30742
                     /locus_tag="LZB08_000152"
     CDS             30395..30742
                     /locus_tag="LZB08_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313673.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEHQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            30742..31167
                     /locus_tag="LZB08_000153"
     CDS             30742..31167
                     /locus_tag="LZB08_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBM7313674.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGMAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            31160..31597
                     /locus_tag="LZB08_000154"
     CDS             31160..31597
                     /locus_tag="LZB08_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313675.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPHSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            31590..31766
                     /locus_tag="LZB08_000155"
     CDS             31590..31766
                     /locus_tag="LZB08_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313676.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            31767..33077
                     /locus_tag="LZB08_000156"
     CDS             31767..33077
                     /locus_tag="LZB08_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBM7313677.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYV
                     IWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDTELLESSLIYWTTGAIAGC
                     DINKSNTNKTYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            33094..33564
                     /locus_tag="LZB08_000157"
     CDS             33094..33564
                     /locus_tag="LZB08_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBM7313678.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            33636..34076
                     /locus_tag="LZB08_000158"
     CDS             33636..34076
                     /locus_tag="LZB08_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBM7313679.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            34417..34578
                     /locus_tag="LZB08_000159"
     CDS             34417..34578
                     /locus_tag="LZB08_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313680.1"
                     /translation="MNNFLLNVIAGVIASLIFCIICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     repeat_region   35316..36353
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=35316..35344
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            36513..36683
                     /locus_tag="LZB08_000160"
     CDS             36513..36683
                     /locus_tag="LZB08_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBM7313681.1"
                     /translation="MSPKKIGRPVVGSPKTNDIKVRVDDETNEKLDEYCKKNNLTKAE
                     AIRQGIHLLLEK"
     gene            36811..37653
                     /locus_tag="LZB08_000161"
     CDS             36811..37653
                     /locus_tag="LZB08_000161"
                     /inference="COORDINATES: protein motif:HMM:NF014548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBM7313682.1"
                     /translation="MKTNLELIKSENFGEVECNFYENDTKDILMTREQIGMALEYTEP
                     RIAISKIHERNKDRLDKFSTVVKLVTVEGNREVLRDTIVYNTKGVMEICRFSRQPKAD
                     TFMDWVWDVVESIFKTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGF
                     DNKSKLLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEII
                     KKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKIRNWIEENNRPAKIAGEKKNFHVV
                     YKIE"
     gene            37708..37863
                     /locus_tag="LZB08_000162"
     CDS             37708..37863
                     /locus_tag="LZB08_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313683.1"
                     /translation="MYENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKEEKNK"
     gene            38420..39190
                     /locus_tag="LZB08_000163"
     CDS             38420..39190
                     /locus_tag="LZB08_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBM7313684.1"
                     /translation="MGLFGSKDNCCICGEKGKQKIADGWLCKECFKKYAVATFTPGNT
                     LYRGLPTKLEVEKAIESKDDKEKELKNFNPTKKILKFMEFDDNNKKFIVLNGFNREKV
                     NLSVYNYSDVIEYELLENGETVTKGGIGRALAGGALFGGVGAVVGGVTAKRTTKAFID
                     SLKIKITLNNLSNPNVYVNLIQLRTKSNSSIYKMAYSSAQEILSVLAIIVKDNEAVNI
                     QNNSNDAIQQVKGLKELLDLGAITEEEFNTKKKELLNL"
     gene            39248..41599
                     /locus_tag="LZB08_000164"
     CDS             39248..41599
                     /locus_tag="LZB08_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBM7313685.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     AGSIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKATDTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLAAKIADAMGKTKDQLKELSSSGAITADVIRNALFKASNEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLGKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITLGAVLIIQGLGGITNAALSVVNVFAQNWNIIEPIVYG
                     VAAAIGIYTIAAIALAVANKVASLSAAWFNFQMTQTVIMHELATGATWMQVAAQYGLN
                     AALYACPLTWIVLGFIAVIAVVFVAVAAINKFAGTSLTVLGVIVGAVFAAVATIQNIM
                     IGLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAGNALGKAF
                     IDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSLNKWVGDA
                     PEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKKKLGLDDL
                     WNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFT
                     GVNIKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            41616..42311
                     /locus_tag="LZB08_000165"
     CDS             41616..42311
                     /locus_tag="LZB08_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBM7313686.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPAQPKTTNETSRPNVKIE
                     PKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKNLS
                     KYTIYKGQVLRLV"
     gene            42304..44196
                     /locus_tag="LZB08_000166"
     CDS             42304..44196
                     /locus_tag="LZB08_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBM7313687.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGVTWEIERRGTPSK
                     LEFTIVMDDILQFCEGNSVRLYYKGTGVFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     AFGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            44210..44470
                     /locus_tag="LZB08_000167"
     CDS             44210..44470
                     /locus_tag="LZB08_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBM7313688.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            44475..44894
                     /locus_tag="LZB08_000168"
     CDS             44475..44894
                     /locus_tag="LZB08_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBM7313689.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            44895..45944
                     /locus_tag="LZB08_000169"
     CDS             44895..45944
                     /locus_tag="LZB08_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBM7313690.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLSATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            45937..46554
                     /locus_tag="LZB08_000170"
     CDS             45937..46554
                     /locus_tag="LZB08_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBM7313691.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITSKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            46566..47591
                     /locus_tag="LZB08_000171"
     CDS             46566..47591
                     /locus_tag="LZB08_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBM7313692.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQIIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARS
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEISKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            47608..49149
                     /locus_tag="LZB08_000172"
     CDS             47608..49149
                     /locus_tag="LZB08_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313693.1"
                     /translation="MATSMNNFNLPDMRKYTKNIAFDPFAGGRQSFDWGGNNHGLQGS
                     SKDTCLSCTYNGSTINWGSGNVWVLGGYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPDVNLIVRVYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSIDGNHKLKIQLNDGYDNFDRIFTFKKIEKGINISTSLVTDSQAKF
                     TVTKTYSELTKIEWYLDEVLKDTFTTDLYSEKTINYELTDNAIHTLRIVATDAENIVE
                     EKVISISKNIMPLQPNASLSDISTKLTEIGQGVRNGKTSIINTLALKNIEASLNNTLV
                     ELSEKIKQSFDSGDASLQDLTSKLTQANNTISQLNTKYKYASGSAATKMNAADTAYLY
                     DTGVYNPDYPQKPIDWLNIKGLNFIPNVFFAECECKLNSPTKFLKYLVFAVCSLPSIS
                     SDKDFTVTVLLDKTYGNQKYTANGQVYINNKGNIYMNNEGIYIPAYNPSLSYTLYNWH
                     AIKFI"
     gene            49163..49456
                     /locus_tag="LZB08_000173"
     CDS             49163..49456
                     /locus_tag="LZB08_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313694.1"
                     /translation="MNRANRIIYDQTGKILFQTGEATGDILEHDTITELHYIDVGYGN
                     IDYSKQYIESINPITKELILKDIPIYLSEEEKRIQELENQLLIAENEKVGGLL"
     gene            49456..49638
                     /locus_tag="LZB08_000174"
     CDS             49456..49638
                     /locus_tag="LZB08_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313695.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNTDYRTAVEN
                     YIIEHSGVVEGIEPTA"
     gene            49672..49863
                     /locus_tag="LZB08_000175"
     CDS             49672..49863
                     /locus_tag="LZB08_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313696.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENNFTSDNVEGALKELATE
                     VNGQRAKGITIANDLIDMI"
     gene            49877..50509
                     /locus_tag="LZB08_000176"
     CDS             49877..50509
                     /locus_tag="LZB08_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313697.1"
                     /translation="MTEKLTDNASLRELMTTLQGVQTDFQNGKSNITTVLGSPFLNTD
                     KLDITKTKIETLKSALVNNLIYKGVSASTQSSFTDLINAINFIVQSILIVKCKGTGRI
                     EEVNNPYVIYNDKPNIKGCLRIKGTLEVYKNWATFASARLEIIYGEKKEYIYLSCDAT
                     ASGRSTNFEKDIIVNNDTQLKIQVLLTDVGTGNSSTAKAYTSVSDVTLLR"
     gene            50520..50723
                     /locus_tag="LZB08_000177"
     CDS             50520..50723
                     /locus_tag="LZB08_000177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313698.1"
                     /translation="MKKQFYYDSLEEKEKIINENNNLYVIELSETLKGKYIILSDNPI
                     YEKLSYEELENELLTNELKGEIL"
BASE COUNT        20345 a         5491 c         9455 g        15432 t
ORIGIN      
        1 tgagtatgaa tttctaataa aaataaatat cataaaaata agaaaaagta ttgggaggaa
       61 atgaaaatgg caagaatgta ttatgaaaaa gatgtggatt tagaggtttt aaaaaataaa
      121 aaggttgcag ttttaggtta tggaagtcaa ggacatgcac atgcacaaaa tcttagagat
      181 aacggtgtac atgtaatgat aggtctttat gacggaagta aatcagcaca aaaagcaaaa
      241 gaagatggat ttgaagttaa gagtgtagca gaagctacaa aagaaagtga tttgacaatg
      301 atgttaatgc ctgatgaaaa acaaaagaaa gtttatgaag agagtgtaaa ggataactta
      361 aaagaaggtc agacattagc ttttgctcat ggattcaata tacattataa tcaagtacaa
      421 ccaccagaat ttgttgatgt tgtaatggta gctcctaaag gacctggtca cttagttaga
      481 aatgtattta caaagggaag tggagttcct gcactatttg cagtatatca agactacact
      541 aaaaaagcta cagagacagt tttagcatat gcaaaaggga taggagcaac aagagcaggt
      601 gttttagaaa ctacttttaa agaagaaaca gaaacagatt tatttggaga acaatcagta
      661 ctttgtggtg gaataagtga acttataaaa ttgggatata aaacgcttgt agatgcaggt
      721 tatcaaaaag aagtagcata ttttgaatgt ttacatgaaa tgaaactaat agtcgacctt
      781 atatatgaag gtggatttga aagaatgaga tatagtataa gtgacacagc agaatatgga
      841 gattatgtat ctggtaaaag ggtaataact gatgctgcaa aacaaggtat gcaaaatgtt
      901 ttagaggata tacaaaatgg aaaatttgca aaagcgtgga ttaaagaaaa tgaagaagga
      961 cgtgaaaact tcctaaaaac tagagaagaa gaatataata cagaaattgc cgaagttggt
     1021 agaaatctaa gaagtatgat gtcattctta aaataaatat atattaaatg gattagtttg
     1081 agggaaagag tggtgtatag tagtgtgtat gaatggagca aaggtaattt tggaatgttt
     1141 aaagaaagag ggaattgaca caatatttgg atatcctggt ggagccgtga tacctcttta
     1201 tgatgctcta tacgattatt cagatgattt taaacatata aggacatccc atgaacaagg
     1261 tttagttcat gctgcagatg ggtacgcaag aagtactaat acagtaggag tttgttttac
     1321 aacatctggt ccaggagcaa caaatgccat aactggaata gcaacagctt ttatggattc
     1381 ttcacctatg gttgtaattt cagggcaggt gccaactagt ttattaggaa aagattcttt
     1441 tcaagaaatt gacataactg gagcaacatt atctatgaca aaacataatt atttagttag
     1501 aaacactaaa gaattagttc ctacaataaa agaagctttt agagttgcaa attcaggtag
     1561 aaaaggacct gtacttgtag atgttccaaa agatttattt ttagcagaaa tggattttag
     1621 tggtgaggac tatgatttat gtcaaataga tgattatatg gattataaga gtgattttga
     1681 tctagatgat gaaactaata ttaagctttt aaatgaagca atagatatca taaaagaatc
     1741 taaaaagcct gtaatatatg caggtggagg tgttaaatct tcagacagtg aggaaattct
     1801 tgaaaaattt gcaactaaga tagatacacc cgtactaaat acacttatgg ggcttggaaa
     1861 tatagataga aagaatgaac tatctcttgg aatggttgga atgcatggaa gtagagaaag
     1921 taatttagca ctttctaatt cagatctagt gatagcgata ggtgcgagat ttagtgatag
     1981 agtgataagt aaaagttctg aatttgctaa aaatgcaaaa ataatacaca tagacataga
     2041 cccatctgaa ataagtaaaa atatagaatc taatgtatct ttggttggag atgtaaagct
     2101 agtccttagt ttattaattg aaagggttga aagtaagaat aatagcaatt ggaaagaaga
     2161 gataaagaca tttagaaaga gtgagggtgt acaaacatat gaatttcatc cacaaaatat
     2221 acttaaaaaa ataaatgaaa aatatgaaac attaaagaaa cccactgtgg tagtaactga
     2281 tgtcggtcaa catcaaatgt gggctgctaa gtactggaat tttaaaggta ataaaagttt
     2341 tataacatca gcagggttag gaactatggg atttggttta ggtgcagcta ttggaactaa
     2401 agttggtaat gtagataaaa atgtagtttt agtcactggt gatggaagtt ttagaatgaa
     2461 ctgtaatgaa ctagctacag ttgcaaatta taatgtacct atgcttattt tactactcaa
     2521 taatagaaca ttaggaatgg taaggcaatg gcaaaagtta ttttccaatc aaagatactc
     2581 tcaaactgac attaatgaaa atgtggatta tgttaaactc gtaaatgcat ataatattga
     2641 tggatataag gtagccagta tggaagaatt aggaaaggcg ctagatgtga tagattttaa
     2701 taaacctgta tttttacaat gtgatataga taaggattat gatgtttatc ctattgtagc
     2761 acctaatgat gcattggaaa atttgatatg taactaaaac ttatactgaa tattgataaa
     2821 attttttatt aaatttttat tggtattcag tttttgttgt ttagagatag attggcattt
     2881 tttatttttg caaataaatc aataactata taattaagaa cttattaaat tttaattata
     2941 caatttagaa tagtattaca acttaagtaa atattaagtt ttatttatta gatttattat
     3001 taatacctta tattagttat ggttgttata tactctaaag ccttgaaatt acaagcgtat
     3061 agctgactat ataaatttga taattttagc ttttaaaata gataaattag attttgccac
     3121 ccatttgcca ccgtattata tttttgggtg gcaaactcta atttgtaatt ttttcaaata
     3181 tatctacagt ttcatttttc attttatcag ttacatgtga ataggtatcc attgtagttg
     3241 atagttggct atgacctaaa cggttttgta tgtctttaat gttagcacca ttttctaata
     3301 atagagtagc atgtgcatgt cttaaagaat gaaaatggaa gtcattgttt aaagctactc
     3361 gaatttgtct tactatagtg tctaaagtgt gagtattcac ttgttgacca ttttctttgg
     3421 tacataccca atcactatca aagtaaaatt ctccatattt taatttcatt ttcttttgat
     3481 ataatttatg ttcttttaat gcctttatta aagtgtcacc tgtaaatata gttctgcaag
     3541 agctttctgt ttttggttgc cctaattcaa acattccatt tggtttttta atcaaagtat
     3601 gctttactgt gatagtttta ttatcaaggt ctatattatc ccattttagt gcaataattt
     3661 cacctcttcg cataccagta tgaaatccaa ttagtaaaac tatacgttga aatgaatctt
     3721 gaggaaatat atttagtatt tgattaaatt cttctaatgt aatagtttta actttattag
     3781 tttctgtttt agatttagtt tttggtatgc ttacatattg catagggttt tctcgtatgt
     3841 gtttgtaagg atggactgct gattttaatg acctatgtaa tatggctttt aatacttgta
     3901 atgtattttg agagtaatcc tctttgtact ttttatttat gaagttttgt agtattgcag
     3961 gagttaaagc ttttactttg taagctccta gttttggctt tatatgtttt tctatgttta
     4021 ttcggtagct ttcttgagtg ttgtatttac agttaagtaa gacatattct ttgtaccaaa
     4081 agtctaagta gtctgataaa ctgatattgc tttcttcaaa tactatgcca gagttttcat
     4141 attcatttag tgcttctctt aaggcttttt cggcttcttt tttagtattg cctccaactc
     4201 tttctacttt ttttcttttt ccttctacta tgcctaggtc aaagtagtaa taccatttgt
     4261 tacttctttt tcttactcca cctttcataa tagtatccct cccttttaga atgtatgttt
     4321 gtttggtgtt tatataaaag agcagattaa ctgctcttta tatacttttg ttatgtacaa
     4381 tattatctta attttgttta tctggttttt acttaattat tttcttaata ttcttacaat
     4441 tttttagctt ttatgtgtag tttattataa taatattgaa atatatattt attaagtaaa
     4501 aaatctataa aagtagaata aatgagttta aaaaatatcc tcatatatgt agaaatttgg
     4561 caaattagtt aatactaaaa atctattatt tcctaagtcc aacatgcttt tcttttttga
     4621 tagaaattct aatcttttta gtaagaagtt gatgctaact tgtaattctt cagctatttc
     4681 gtatacactt gtagcgtgtg aattaataac atgtattatt tcttcttctg ttataagaaa
     4741 ttcacatgcc cattttaatg ctttgttttc agttttgtct atcaagattt tatttttgta
     4801 actgtttttt gaagatacat agttcccaac actggtaaaa tgatgtccta attcttctgc
     4861 taagatttct attagtttag cattgttttg ttttaatgaa ttaagtaatg atataatctt
     4921 tagtccttgt ctgtttatat acaatccttt tatgtcatct gctattttgt cagtgtagta
     4981 aatttctatc tcttcattat ttgctaagtc taaaagtgcg tctagtttgt tcattgaaat
     5041 ccccctataa aaagaatgta tgtgttgctt ttttatatat aaagagcagg agtgaactgc
     5101 tctaaatatt cattttttat atctatcaac taaaaactct atgtagctat taatatgttc
     5161 ttgcgcttct ttaggtaaac tttcataaga tttaattatt ttggatattt catcagtagt
     5221 tatgtaattt tcaattgagg ttttaccaag tagataatca actgacacat taaaaaattc
     5281 tgaaattctt aacaagtctt ctttaacagg ttctcttttg ctagtttcta acatagcaat
     5341 tttgcttagt gaacaattaa gtaattttga taattctgct tgagttaatt cttttttctt
     5401 tcttaattct tttaacctta ttggaaaagt tgaatttaag cattttgtta atttatttga
     5461 atcatctatg ttaatttttc caattaagta atctgtagat actccaaaat agtctgagca
     5521 atctttaata aatgattctt ttggttctct taacccattt tcaattctgg ataaagtgga
     5581 tttatttata tttaagtctg tgcttaattt atctaaagaa attccttttt cagttctcaa
     5641 tttttttaat attaacatag attcaatcct tattttttat atttattcat taaaaattca
     5701 atataatcgt tgagttgttc ttgagcttcc tctggtaaat ctttgtgagg attaactctg
     5761 tgtgcggcta cagtatcgat gtgatttcta acaagagttc tacccaaaag ataatcaatt
     5821 gatacatcaa aaaaatctgc ccaattttct agaatattta tttctggttt tcttttatta
     5881 ttttcatatt gcgatattgt tgatttatta aaacttgtaa agtaaacttt attgaatttt
     5941 gaaactagtt catcttgagt aagttttttc tcttctcgta attgtttgaa acgttctcca
     6001 aaagttgcca caaacaacac ctcccaataa ttaaatgata ctataagttt acagaaacgt
     6061 aaacttatgt ttagaaaaaa ataaaaaagt taacaaaaaa ataaacttat gtattgacaa
     6121 gttttgtttg tgtgaatata ataaaagtat gaagttcaca aaaataaaac attggaaggt
     6181 gaagaacatg aagaaaagaa gtttaaaagc tggtcgaata gaagcaggat atacccaaga
     6241 agaactagca tataagatag gaatagcaaa atcaacatat aatctaaaag aaaatggaaa
     6301 aagaaatttt acggaaaaag aaatgataat gattagctat attttaaata aaacaatgga
     6361 tgaacttttt ttagaaaaaa ggttaacata aattaaaatt tattgttcac aaaaacaatc
     6421 ataaggagga aagaaattat gaataattta caaatattca aaaataagac ttttggagaa
     6481 ataagagtaa tcgaactaaa tggagaattt tggtttgttg gaaaagatat tgcagaacaa
     6541 ttaggatata aagatacatc tgatgctttg aaaagacatg ttgatgatga agataaagga
     6601 gtaggtgaaa taccgactcc tggtggtaat caaaatatga aagtaattaa tgaaagtggt
     6661 ctttattctt taatattaag ttctaagttg ccaagtgcaa aattatttaa acgttgggta
     6721 actaatgaga tattaccaag tatacgtagt actgggacat ataatatgat agatttgcaa
     6781 cctaaattac caactacata taaagaagcg ttacaacatc ttatagagca agtagaagta
     6841 aatgagaaat tacaactaga aagtaaaatg aaagaaaaag taataaaaga actaaaacca
     6901 aaggcagatt atacagatat gatacttaaa aacaaaggtc tagtcactat aactcaaata
     6961 gcaaaggatt atggaatgag tggaaaagaa atgaacaaaa tacttcatga aagagggatt
     7021 caatataaac aaagtggaca atggctttta tataaacaac atcaagggaa gggatacact
     7081 cattcagaaa caatagacat aactagaagt aatggaatgc ctgatgtaaa aatgacaact
     7141 aagtggactc aaaaaggtag attgttttta tatgatttat tgaaaacaaa taacatatta
     7201 ccagatatag aaaaagaata tagttatcaa acttcaatat taggttagta ctttgaaaac
     7261 taaatacaga atattttgga aaggagagat tgaattggaa aataagaaaa tagaagaaat
     7321 agagaaaaga ataaccatgc ttgaaaatca attgcaagca cagttattta gtcagataac
     7381 agctcaatca aatagtgatt ttatagatta tatgtaccaa tgtatagttt tgtatttaca
     7441 tgagatgaat aaaagtgaaa aaagaaaaat tagtgaaatt actaaagaaa gaatcagaaa
     7501 ataagaatga taattacaat ataaaaccta ttcttctagc aggttttgaa gggtcagttt
     7561 tctttactga agttatcata aagtttatct gtgatgtgtg ttgaagaatt tcagaataaa
     7621 ctccatttac tatgccgtag tagtgaatta gacaagggtt atgataacct acttcttcaa
     7681 caatcattaa tacatctttt ccaaagttag tcaattttag tgcaatttca tgttcatcat
     7741 caagttcttt ttcaaattcc tctatagatt taagaataat tttatattga aattcagcat
     7801 agtaaaaata ctctgtgtta gaagtatcag agactgtctt tcttatactt tcttgtaatt
     7861 ctttagatat attgtttgaa aactgcataa tacaccatcc ttttgaaaat tttggaattt
     7921 attccatgtt tatattatac catgtagaac tgaggtgaat acaatgttaa taggcgacaa
     7981 tataaagcaa atactaagaa aaagagatat aaaaccttat aaattggcaa aagaattgga
     8041 tatagatgta agtggtttat ataaattact gagaaataaa agttctaatc caactataga
     8101 tactctaata aaattagctg attatttaga tattacatta gacgaattgg ttggaagata
     8161 aataattgaa aagaggggag agcattttca catggatatt tctgaaagca taacaaaaca
     8221 atttagtgat agcttaaaaa gcttaataga aatagagata agtaaacaag agacagatag
     8281 agttaagagt caaactattg aacaaaaggt aaaggtactg gagcctaaag atatagttgt
     8341 tttaataaaa agaggttatc caaattatct aataacagta gaagaagcaa ggggaatttt
     8401 aaagttagat acagctttta tgcgtaggtt agtaagcaca ggtttgataa aatcactagt
     8461 tagaggtgat ggtagaaaaa tttcaagata tgaagttgat gattttattg aaagaaatca
     8521 aggtaaaaat ttggatgaac ttttaaaagc agtagagagg ggggatgaaa ttgctgagtc
     8581 ttgatactaa taataagaat gtagtaactc ttaaaaaaga tggaaaagtt atagcagata
     8641 tagtatttaa agatattaaa actggtaaga aaatatcggt tggaatatta aataaaaaag
     8701 tgttggtaaa ataaccaaca cacaaaaaaa taaaaaataa aatataacac aagtaaagta
     8761 taacacaaaa aggagagatt taaaatggtt gaagtgaatg taaatgtaaa agttaaagta
     8821 gaagcaccag aatttacaaa tgcactatta gtagtggcga atgctttagg aggtcttaat
     8881 ctaggaaaag caatgcagat agaacctata aatataacag atatgaaaga agagaagaaa
     8941 gttgaagtta aaaaagctga aaagattaaa aaggtagaag taaaagaaga aatagcagaa
     9001 gcaaaggaag aaatagaaaa gaataatgaa aatacaacta gtgaagttaa atacacaaaa
     9061 gaagaggtaa gaactaaggc agcacaagta agtaaggcag gtaagaagga taagcttaag
     9121 gagttatttg gtgaatttgg agctagtaag ttaagtgaag taaaagaaga agattattca
     9181 gcttttatga acaaattaga aagtttatag ggggtacata aatgccatta caacatgcaa
     9241 gacttagtgc gagtggagcc catagatggc ttcactgcac tcctagtata aaattagaag
     9301 aaaactatcc accatcaact agtatatatg cagaagaagg aacagttgca catgaattag
     9361 cagaagttaa attaatgcta gagtatgaaa aaataagtaa aaaggcatat aatgcaagaa
     9421 ttaaaaagat acagaaaagt gagtattata actctgaaat ggaggactat atacaatctt
     9481 atgttgagaa tgtagttgaa ttagtaaatg atagtaaagc tatatgtgat gatgtaatag
     9541 tgatgttaga agaaagactc gattttagtg agtgggttcc agaaggattt ggaacaggag
     9601 acgttgttgt aatatctgat ggtatacttc aagttataga cctaaagtat ggaaaaggtt
     9661 tagaagtttc agctatagag aatcctcaac ttagactata tggtttaggg gcatataatc
     9721 agtttgaaat gctatacgat attgatttaa ttaaaacaac aataattcaa cctagacttg
     9781 ataacatatc aagtgaagaa atagaagtta ctaaattact tacatgggca gataatgtta
     9841 agaagaaggc tcaaatggct tttaatggtg aaggagagtt tgtaagtggc agtcattgtg
     9901 gattttgtag agctaaaaat gattgcagaa aaagagctga ggataacctt aaactagcta
     9961 gaaaatatga ttttgctgac acatttgctc ttaataaata tgagatagca gatattttag
    10021 gttttgctaa gaatatacaa gattggttaa aagatgttca aagttatgca ctagaacaag
    10081 ctgaaaagca tggtgttaaa tatccaggat ataagcttgt agaaggcaga agtaatagaa
    10141 agtatgtaga tgagcaagaa gttgctaagg ttttattaaa ttcagattat gatgaagaaa
    10201 aaatttataa gccaaggacc ttaaaaggaa ttagtgatat ggagaaagct ataggtaaaa
    10261 agagttttgc taaactacta agtgatttaa ttataaaacc agttggaaaa gctactttag
    10321 tagtagaaag tgataaaagg tcagaaatta atagtataga ttcagcgaaa aaagattttg
    10381 aaatataaat gggggatatt acatggcatt agatgcaggt ataaaagata aattagatga
    10441 aagcataagt ataattttag atttaaagga tagaaaaaag agtatcgaaa aagatttaga
    10501 ttatgaaaaa gataatttac tagatatgtt aaagatacta aatattacag aatataactc
    10561 aactgaagaa aatgcaaaag ttatagtgat ggattttaaa agagaaagtt taataaagga
    10621 taaagtgatg tcaactttcc tcgaagtaaa tgacaatcat cttgatagag tgtatatacc
    10681 agaacatata aaagttagtc cagtatgctt tgtatcagta agagcaaagg ataattaaaa
    10741 atatttaatt cctagtagct ataaaaagat ttaaaagggg atataatttt atgagtaatc
    10801 aagaaataac taaactagtt gaaaataatt tgaatcttgt acattttagt ataaatagat
    10861 tttttaagtc ttatgtagag caacatccat acttatatga agagttttat caagaaggat
    10921 gtattgggtt atataaagca gcattaaatt atgatagtaa cagaggcaag ttttctacag
    10981 tggctgtttc ttacataaga atagaaatgt ttaaagcttt gcgttatggc gaaaaacatt
    11041 atagaaactc tatagaaggt ttagatgtaa gtgtatttga agatagtaaa agaccattaa
    11101 aagatataca tggattttat gaaattcaat ttaactatga tgttgattat ataagaaaaa
    11161 ttgcaagaaa gtcatattta aaagatattg ataaaatagt agattggatt ttacaagata
    11221 aaaagatatc tgatatagga gagttattaa atgtttcttc aacagttatt tgtggaagaa
    11281 ttaaattgtt tagtaagcaa gttagagatt tagataaatt tggagaaata acttcaaata
    11341 taaaatcaat ataccaaata taaaaatatt aggaggaaga aaattatgag taattcagta
    11401 caatcaacaa aggtagtaac aggaaaggta agattaagtt attgcaatat ctttaaaagc
    11461 agagcaatgg tagaaggtgc agagcccaag tattcagttt gtattttaat accaaaatca
    11521 gataaggtga ctttaggaag aataaagaag gctattgatg cagctaaaga acaaggtaaa
    11581 acttctaaat ggggtggaaa attaccaggt aatttaaaaa cacctcttcg tgatggagat
    11641 gcagaaagag ctgatgaagc agaggaatat gtaggaatgt attttttaaa tgcaaatagt
    11701 actcaaaagc caggaatagt tgataaagac ttaaatgaaa tattagataa tacagaggta
    11761 tatagtggtt gttatggaag agttagtata aactttttcc catataatag tgcaggaaat
    11821 aaaggaatag gttgtggctt acaaaatgtt caaaagttag cagatggaga agtacttgga
    11881 ggagctagag ctagtgcaga agcagatttc tcagatgact ttgaatatga agatgaagaa
    11941 gaggacttct taagttaatg aggaccttat caattgatat agaaacatat agtgatttag
    12001 atataaaaaa agttggagtc tacagatatg tagactctgc taattttgag atactgttat
    12061 ttgcctatgc ttttgataat gaagaggtaa aagttattga tttagtaaat gatgaagagt
    12121 taccaaaaga agtaatagaa gctttaaatg ataataaagt tataaaatca gcatttaatg
    12181 ctaattttga aagaacatca ataagtaaat ttttaaatat taatttaaaa ccaaatgagt
    12241 ggtcatgtac aatgataaag gcgttaacac taggacttcc aggaagttta gatagtgtgt
    12301 ctaaggcttt aaagtttaat gaagataaac agaaaatgaa agaaggtaaa gcattaatac
    12361 aatatttctg taaaccttgt aaggctacaa aagttaacaa gggaagaact agaaatttac
    12421 caatacatga tatggaaaaa tggaataaat ttaaagaata ttgtaaacaa gatgttgtag
    12481 ttgaaagaga aataagaaac aaacttagta agtataagac taccgaaaga gaaattaaat
    12541 tatggtattt agaccagaga attaatgata ctggtattaa agtggataca gagttaatag
    12601 agaatgcaat agaatgtgat aaaagatata ctgaaaaact tacaaaagaa gcaattaaaa
    12661 taactggtct aaataatcca aatagtccag ctcaattaaa aaaatggtta agtgataaag
    12721 ttggctttga gattacaagc ctaacaaaag aaagtattcc agaaatatta aaacaagttg
    12781 atgatgaaaa tgtagttaga attttagaac ttagaaaatt aatgtccaaa acttctataa
    12841 agaaatatga ggctatgaaa ttagctaaag gtaatgacaa tagagtaaga ggtctactac
    12901 agttttatgg ggctaatagg actggtagat gggcaggaag attagtacaa gtacagaatt
    12961 taccacaaaa tcatatagag gatttagacc tagctagaaa cctattaaaa gaaggagatt
    13021 ttgatttaat agagctttta tatgatagtg taccagatgt cttaagtcaa ttgataagga
    13081 cagcttttat accaagtgaa ggtcatagat ttatagtgtc agattttagt gcaatagaag
    13141 ctagagttat agcttggctt gcaggtgaga agtggagact ggatgtgttt aattctcatg
    13201 gaaaaatata tgaagctagt gccagtcaga tgtttaaaat tccaatcgaa aatattaaaa
    13261 aaggttctga acttagacaa aaaggaaagc tagcagaatt atcgaatgga tatggcggaa
    13321 gtgtaggggc cttaatttct atgggggcta ttaaaatggg gcttaaagaa gaagagcttc
    13381 aacctattgt tactgcatgg agaaatgcta atccaaatat aactaagttt tggtgggatg
    13441 tagataaggc agctaaaaaa gctataaaag ataggactat agtagaaatt caacatggga
    13501 ttaaatttat ttataatcca ggcgttttat ttatagaact accaagtagt agaagattat
    13561 cgtatctaag gcctaagata gagccacata ctacatttag tggggataag ataacatatg
    13621 aaggtatgga acagacaagt aaacaatgga aaagaataga tacttatgga cctaagttag
    13681 ttgagaatat tgttcaagct acagctagag attgcttaag agaggctatg tttaatgtga
    13741 cagatgcagg ttatagcatt gtaatgcatg tacatgatga gcttgtaatt gatgtagata
    13801 aaaaaggagg ttctttagaa gaagttaaca gtattatggg aaaagaaata tcttgggcta
    13861 aaggtcttcc tcttaaagca gatggatatg aatgtgatta ttataagaaa gactaggtga
    13921 ttgatttatg gatataaagg ccagtgaaat tgaacacata aatgtaagac atgatggcca
    13981 actcatgctg gccataggaa aaaataaatt agaaacacat tggaaaaata aaagtatttt
    14041 atggtccgaa cttgtaaata gattaagtaa aacattaaga actcaagaaa catatacaga
    14101 atataggaag atgtcaaaga ctgaaaaaga tagagttaaa gatgttggtg gatttgtagg
    14161 tggaagctta aaaaatggaa gacgaaaagc agagaatatt gcaaatagaa gtattataac
    14221 tttagatata gattatgcga ataaagatat atgggaggat ataacattat taaatgatta
    14281 tgcttgtctt atgtattcta cgcactcaca tactgagaat aatcccagat atagacttgt
    14341 aataccttta actagaccag tactaccaga agaatatcaa gcaatttcca ggatgatagc
    14401 agatactata ggaatagata tgtttgatga tactacatat caacctcata gacttatgta
    14461 ttttccaagt acttcaattg atggagacta tatatttaaa tttcaagatg gagagttttt
    14521 aaacccaaat gaaatactag atttatattt agactggaca gatgtaagct attggccaga
    14581 aagttcgagg gagaggcaaa agtttaatac acaattaaaa aaacaacaag accctattga
    14641 aaaggctgga attataggtg cattttgcag gtcctatagt ataaaagaaa ctatagaaac
    14701 tttcttaaat gaagtatata ttcctggtat tgatgaaacc aggtatacat attcagaagg
    14761 tagtacaagt ggtggagtag ttatttatga tgacaagttt tcatatagtc atcatggtac
    14821 agacccagca agcggaattt tatgcaatgc ttttgattta gttaggatac ataaatttgg
    14881 tgaacttgat gaagatgcta aacccgaaac gcctgtaaat agattacctt catttactcg
    14941 aatgagcgaa tttgcaagca gtgacactaa agtacgaaag actataggaa gagaaaacct
    15001 tgataaagct aaggatgatt ttggtgatat agattttgaa gatgatgaat ggttaactag
    15061 gttagattat gacaataagg gaagttataa gaaaacaaca aacaatatct taatgtttat
    15121 agaaaatgac ccatatttga aaggaaaaat agcttataat gaattttcaa atagagctgt
    15181 cgttttaggt aagttacctt ggagaaaaga tgataaatta aatgattgga atgatagtga
    15241 tgattctggg cttagacatc atatagaaac aatttacaat atctcatcac catcaaaagt
    15301 aaatgatgct ctaataattg cttttgaaaa taatactttt catcctataa aagattattt
    15361 aaattcttta aagtgggatg gcattaagag agtggataca cttttaattg actatttagg
    15421 tgcagaagat aaccactatg caaggactat aataagaaaa gttttagtag cagcagtagc
    15481 aagagtattt aatccaggaa taaagtttga taatatgatg gttttatctg gtcctcaagg
    15541 aatgggaaaa agtactttta ttaaaaaact tggtggagac tggtattctg atagtttaac
    15601 tactgtacaa ggtaaagaag cgtatgaaca attacaagga gtatggttgc tggaaatggg
    15661 tgaaatgatg gctactaaaa aagcagatat tgaggcagtt aagcattttc taagcaaatc
    15721 agaggatata tatagggtcg catatgggaa gagaacctca agatttttac gtcaatgtgt
    15781 agttatagga acaactaatg ataaagaatt tttaagagat aagactggga atagaaggtt
    15841 ttggccaata gatgcaggag taaagaagat taacaagagt atatttaatg gccaacttga
    15901 taatgaaaga aatcaaattt gggcagaagc agtagaatta tataaagcca atgaacagct
    15961 atatctatca gatgaggaga aaaaagaagc tgaaagacaa caaagaactc actcagaaga
    16021 aaatgctaaa tctggaatta tcgaagagta tctaaataaa cctattacta agaattggta
    16081 tgatttaagt atttcagaaa agagagaata tattcatggt tcagattttg gtgatttaaa
    16141 agaagggaca atattaagag aaaaaacgtg tgttatggag atatgggttg agctatttaa
    16201 tggagaacct aaacaactca cgcctatctt atcaagagaa attaatgata tattaaaagg
    16261 attagatgat tggatatcat ataatggaaa tttaaggttt gggaaaatat atggtaggca
    16321 gagagcttat gtacgtaaaa gttagtgtca acagaattaa aaacagagta aatttgaaaa
    16381 ttttaaaagt gtcaacaatg tcaacaaaaa aaatggcttt gttgacgctt atgttgacac
    16441 ctaagatgca gtaattacaa tgtttgaggt attaatgtca acaatgtcaa caaaaattaa
    16501 gtataaagta gtataagtaa tattaggcat atacgtatat acatatgtat gcctaatatg
    16561 tatatataca tatatataga aaatctgttg acattgttga cacttaaatt ttaaggaagt
    16621 gatattttgt tagaatcaaa aatagaaaaa agacttaaaa aagagattga gttgttaggt
    16681 ggaaaggcta tgaagtttat ttcaccaggt gaggcaggtg tgccagatag gattgtctta
    16741 ttaccagaag gacatgttat atttgtagaa cttaaagcac caggtaaaaa accaaggaag
    16801 cttcaacaat ataaaatgcg agaattaaga gaattaggat ttaaagttaa gtgtgtaagc
    16861 acattaaaag agatagatga ttttatcaag gaggttaagg gatggaattt aaaccacatc
    16921 cataccaaga atacacaatt agaaaaacta tagataataa taatataggt ttactattag
    16981 atatgggatt aggtaaaaca gtctgtgctc taacagctat aagtgaatta atgtatgatt
    17041 attttgatat atcaaaagtt ttagttatag cacctttaag agttgcaaga gatacctgga
    17101 gtagtgaagt aaaaaaatgg gagcatctaa agcatctaaa agtatctaaa gttcttggta
    17161 gtaaattaga tagagtgaga gctttaagta cagactcaga tatttatata ataaatagag
    17221 aaatggtacc ctggatagtg gatttttata aaagaaaatg gccatttgat atggttgtga
    17281 tagatgaact tagttctttt aaatcaaata aagcacaacg atttaaaagt ttaaagaaag
    17341 tattgccttt aactaaaaga gtagttgggc ttactggtac accaacacca aatagtttaa
    17401 tagatttatg ggcacagatg tacttacttg atagaggtga aagactaggt aaaactatta
    17461 caggatataa agaacgatac tttgagccag gacaaaaaaa ttatcaaaca ggagctatat
    17521 ataattggca gcctaaagat ggagcagaaa atgcaataca taataaaata aaagatattt
    17581 gtataagctt aaaagcagaa gattatttga atatgcctag aaaaatagat aataaaattg
    17641 aaatacacct tgatagtaaa atacttaaat attacaaaga attagaaaaa gagaagatat
    17701 tagaattaga aaaagatata ataacagctt catcagcagc tgtaacagca aataaacttt
    17761 tacaattagc taatggagca atttatgata atgataaaaa tgtaaaggaa ctccatagag
    17821 aaaaattaga agctttaaaa gaaattatag atgtttcaaa tggtaagcct attatagttt
    17881 tttataacta taaacatgat tataatcgtt taatgaaaga gtttaagagt ttgaaaccaa
    17941 gaacaataga aaactcaaaa gatatatatg attggaataa tggaagaata caattattac
    18001 tttgtcatcc agcaagtaca ggacatggac ttaatttaca atcaggtgga agtataattg
    18061 tttggtttgg attaacttgg agcttagaac tgtatcaaca agccaatgca aggctttata
    18121 gacaggggca aagggaaact gttattatcc atcatctaat ctgtaaaggt acaatagatg
    18181 aacaagtaat ggaggctcta gagaataaag ataaaggaca aagtgcatta cttgaagcag
    18241 tcaaagcaaa attaaaagaa tatagggagt gaaggtatga aagtaaaaag aaaagatgga
    18301 acttataagc ccaggtaaaa tagtaagatg tgcttggtgt ggtagaagat tttataagtt
    18361 ggataaatct aaagttaagt attgcagtag aagttgttcg aagagagcaa gaaggagtga
    18421 tttaagtgga taaagaaagt ataaaagaaa ttgtaagaga gttaagaaaa gaagaaaaag
    18481 aaaataaaaa aagagaagtc tttcataata ctaagctttt gttaaagcac tataacgatt
    18541 taaaaagtca tattgcaaat tctattagtg acattgaaga tgttaaaagt gattatgatg
    18601 atttattatg tctagatata gaggatttat atatattaag tatcaaaaga agtaaggcaa
    18661 aaaccttaat tatggtagca catatagata tggcattaga aacattaaaa gtaacacaaa
    18721 ataagttgca gtccttagaa aaatacaaag ccttagagtt atattttttt aaagaaaaga
    18781 catatgagga tatagcagag cttttacatt gtggagtagc aacagtaaga cgttgggtaa
    18841 atgaaatggt taaagaactt ggaatatacc tatttggagt agatggtcta aaaataaaac
    18901 tgtgataaaa tgatgatatt ttattgttat tttatataag ttataatgat agtatgaaac
    18961 aagtgtataa tatattcccc atttaaaaag actaagctac cccgacttag tctttttctt
    19021 ttttaaacga aggagaataa aaaatggata tgattaatct agaggaaatc ttttgtaaag
    19081 caaagttgtt agataaaaaa tatataagtt taaaaataaa tataacagaa tacccaagtg
    19141 aagaaataat aattattcaa aatacaaact tcaatagtaa acttagatat tataaagaaa
    19201 tatatgatga taagctaaat cataaatatg tagatggaat aaaaataata aactctacat
    19261 atgcaaatgg ttttaaagag atagaattat ctttagaatg aagacttaag tcaattttta
    19321 ataggaaata aagtaaacaa tgttggagga actagcagta tttaaaatta tttagttgaa
    19381 agagtcatct taaataggta gatcttttgt tacgattaaa ataaaaattg tcccaaatat
    19441 tgactttttg tccctggaat gttaacctaa ttgtaggagg tgacacgtat gaagaaaaag
    19501 aatattttaa atttaatcaa atatcatgca gaaaaaaatg atgcaggatt tagaacagaa
    19561 gcatatgaaa ttgcacgtta ttttgatgga atcgggaatt atcaactctc agaatatata
    19621 atggctctct tatctgatgc aaatacattt tcacctcaaa ttagtgaaga aaatgaagtg
    19681 ttttttaaga gagttgagat aaccaatgaa ccattacctt tgccagaaac aataaaagat
    19741 aatattattg ggatagttaa tgctgttggt cataatgcag gtgtaaacaa attcttattt
    19801 gaaggacctc ctggtacagg aaaaaccgaa agtgttaagc aaatagcacg aatacttgat
    19861 agagaattat ttgtagttga atttgatgca attgtggata gtaaattagg tcaaacttct
    19921 aaaaatattg cattactttt tgaagaaatt cgcaacttat caaatccaag taaagtgatt
    19981 attttatttg atgaaataga tgctatagcg attgatagaa taaattctaa tgatctaaga
    20041 gaaatgggta gagcaacttc ttcagtatta aaaggactcg atgggcttaa tgatagtgtc
    20101 atattgattg caacaacaaa cctgtttaac tcatttgata aagcattaat tcgtagattt
    20161 gattcagtaa tagattttag ccaatacact aaggaagatt taattgatat aggcgaaata
    20221 ttattaaatg atttttttaa taagtttaag tcagcaggta gaaacatgcg tttatttaaa
    20281 aagattctta ggcttttaga tccaattcct tatcctggag aacttaaaaa tttaattaaa
    20341 tcttctcttg catttagtga tcctaataat gaatatgatt atcttaaaag attatacaag
    20401 acagtgacta aagatataca agaaaataat ctcaaggaaa tacaagaaaa aggttttact
    20461 atgagagaac ttgaaatatt aactggtatt tcaaaaagtc aaatatctcg agagttgaaa
    20521 ggagacaact aaatgaacaa tcttctacag ttgaaaggta catttgaaca aaaaagtttt
    20581 gaaggaaata taggttctcc taaacttcct tctaatgcta ttgtaaaaac tgaacattta
    20641 aagaagctaa aggatgattt agtaaaattg gcgagttttt gggaaaaaga aaattttttt
    20701 tcaggtgctc ttgtaagtgt atattataat aaaattgcgg caaaaagtaa tcgtataaga
    20761 gggttacttt caaaaaaagt taaagctaat agtactattg taggggctag atttacaaca
    20821 gaaggtgaga aaaaacatat tattactcac tatattcaac tagatgtgtt atatgaatca
    20881 attagacgac tagatacatg tataaatata ttgattaaaa attttgatgg agcaataaca
    20941 acaaaaataa atgcggatat taattctaaa aaaataaaat ttgaaccaaa attaatttca
    21001 aaaacaaatt ttttacaaat aattgttgat tgctattttg ttgaaaaatt tgatgtattt
    21061 gtagatgaag aagaatttga ggaaaatgca attataacga tttttaaaac agataacaaa
    21121 gcaccagaat tactagaaag aataggaatt gagttaccac atactagtgt aatagatgat
    21181 actacaattc tgatgaaacc tgatgattta agtttattaa agcagaaagc accataccta
    21241 atttctatgg cagtgagtga tttagctcaa ttagatagta catactttga ttttggtgaa
    21301 aaaaatacaa gatctattcc taagcctact aatgaacctg ttataggagt tattgatact
    21361 ctttttgata aaaatgtata tttttctgaa tgggttgaat atcatgatat gattccagat
    21421 gaaattccta aacaactttc tgattataga catggaacag ctgtatcttc tattattgtt
    21481 gatggacctt cttctaaccc taaaatggat gatggatgtg gaagatttag agttcgtcat
    21541 tttggtgttg caacagataa acagtttagc tctttttcta ttcttagaca tattgatgaa
    21601 atagtaacaa aaaacagaga tattaaagtt tggaatttat cattaggttc aaaattagaa
    21661 ataaataaaa attttatttc tcctgaagca gctatacttg atagaattca gtatgaaaat
    21721 gatattattt ttgtaatagc aggtactaat aaagatataa ataatccaca aataatgagg
    21781 ataggtgctc ctgctgactc aataaactcg cttgtaataa attcggtatc atctgataat
    21841 aaaccagctt catattcaag gtgtggtcca gttttatcat tttttacaaa accagatatt
    21901 agttattatg gtggtgagaa acaatcacca atgcatgttt gtacaccaat gggagaggga
    21961 tttgtaacag gaacatcatt tgcagcaccc tgggttgcaa gaaaaatgtc atatcttatt
    22021 aatattttag gtctaagccg agaagttgca aaagcattaa ttatacatgc atctacaggt
    22081 tggtcaaaac aagatatagc ccctacttta attggccatg gaattgttcc aatacgaatt
    22141 gaagatatta taaagtcacc agatgatgaa atacaattta tattatcagg tgtttcagaa
    22201 aagtataata cttataacta taatattcca gtaccaataa ataaagataa acacccattt
    22261 attgcaaaag caactttgtg ctattttcct tattgctcta ggaaccaagg tgtagattat
    22321 acaaataccg agttggatat atcttttggg agattgaatg aaaaaggtat taaaccaatt
    22381 gataataact atcaaagttc tggatatgaa cattttattt tggaagagga agctagaaat
    22441 aattttagaa aatgggataa tataaagcat attcgtgaag tatataaaaa aggaaataaa
    22501 gatagaaaag cttatgataa ggggatttgg ggtgttagtt taaaaacaaa ggaacgtctt
    22561 gaagaaaaat atggtgaagg tattaaattt ggtattatag taagtttaaa agaaattcat
    22621 ggtattaata ggatagaaga atttataaac caatgtttac ttcgtggttg gttggttaat
    22681 aaaattgatg ttcagaatag aattgatatt tataacattg cagaggaaac aatcaatttt
    22741 gacgatttaa attaattaag agattatagg tattatttgt tataggtttg ataatggtat
    22801 ttataccaca caaaaactct ataatataaa ataaatttat attacactaa ctaaagaact
    22861 ccaaatgagt tcttttttta ttcccaaaac gacaaacaaa cgaggtggtg atgtgcaaga
    22921 tgtcaaagaa aaggtaaaac aagattacct aaaaggtatg aaacaaaagg aaatatcagc
    22981 aaagtatgac attagtttaa acactttaaa gtcatggata aaaagataca attggtctag
    23041 tgagaaaaag aagggtgcac ctaaaaataa aaggggtgca cccataggta ataaaaatgc
    23101 tactggtcca cctggtaata agaatgctga aaagtttggt ttcttttcaa aatatctacc
    23161 cgaagaaact agggaattga tacaagaaat atccataaaa gataaatttg atattctttg
    23221 ggaacagata acaattcaat acgcagcaat aataagagca cagaagataa tgtatgttaa
    23281 aggcaaggaa gaaacggtta aagaattaaa gaaatatgaa agcacagaaa atggtgagaa
    23341 gatagagtat gaatttcaat ttgcatggga taggcaagca tcttttctta atgcacagag
    23401 tagagctatg agtgaactta gaagtttaat taaacagtat gatgaaatga ttcataagga
    23461 ttggaatttg gctacagagg agcagaaaac aagagttgag aagttgaaat gtgaagttga
    23521 taacctaagt aaagatgata ttggagatga tgagttgaaa ataagtgtag attatggtga
    23581 tagaaatgat agttagagta aattttaatc cagatttcaa ggaagctaat tttactaaaa
    23641 aaagatacag agcaatgaaa ggttcagcag ggagtggaaa atctgttaat gtagcacaag
    23701 actatatact aaagttagga gataagaagt atcaaggagc taatctatta gtagttagaa
    23761 agtcagaagc tacacataag tattcaacgt atgcagagct tacaggagct ataaatcgta
    23821 tttatggtaa acaagctgat aagtattgga aaactacttt aaatccttta gaaattaaga
    23881 gtaaagttac tggtaactct ataattttca gaggagttaa tgatgcaaaa caaagagaaa
    23941 aattaaaatc aattaacttc tcgaaaggaa aattaacatg ggtttggtgt gaagaagcta
    24001 cagaacttat ggaaagtgac atagacatac tagatgaccg tttaagaggt attttaacta
    24061 atcctaacct atactatcaa atgacattta catttaatcc agtctcagct actcattgga
    24121 taaaaagaaa gtattttgac tataaaaatg atgatatatt tactcatcat agtacttatc
    24181 tacaaaatag attcatagat gaggcttact acagaagaat gcaaatgaga aaagagcaag
    24241 acccagaagg gtacaaagtc tatggtcttg gagaatgggg agaaactggt ggagcaatac
    24301 ttaaaaatta tgttatacat gaatttccta cagaatttga gtattttgac aatatgaggt
    24361 tatcacaaga ctttggattt aaccatgcaa atgtagtact tagaattggc tttaaggatg
    24421 gagaattata tatatgtaat gaaatatatg tacatgaaat ggatacttca gaaatcataa
    24481 agattgcaaa tagtagaggt ttagaaaaga ctctatttat gtactgtgat agtgctgaac
    24541 cagatagaat taagatgtgg aagagtgcag gatataaagc taaaggagtt aaaaaaggac
    24601 caggaagtgt taaagctcaa atagattatt tgaaacaatt aagaatacat gtacatccta
    24661 gctgcactaa taccataaaa gaaatacaac aatggaaatg gaaacaagat gaaagaactg
    24721 gattatatct tgatgaacca gttgagttta tggatgatgc aatggctgcg cttagatatt
    24781 ctatagataa taagcttaaa aataatggaa taagcttctt aaagtaaagg aggtgttaaa
    24841 tatttatata agtgaaacag atttaataaa agttcagtta aaaaaagaga gcacctttaa
    24901 cctagtaaaa gtcatagagc attatatttt aaaacataga ccagagaaat ataaacaagg
    24961 agaagaatac tattatggta atactgatgt aaacaataag agaagatatt atctcttaga
    25021 tggagctaag gttgatgatt ttactaaggt taataataaa gcaattaaca actaccataa
    25081 gcttttagtt gaccaaaaag tgggctatag tgttggaaat ccaatcgtat ttaatgcaga
    25141 tgatgataat ctcactaagc ttttaaatga cttactagga gaagagtttg acgatacaat
    25201 aacagaacta tatctcaatg ctagtaataa aggggttgaa tggttacatc catatattaa
    25261 tagaaaaggt gagtttaaat atgtaataat tccagctgaa gaagcaattc ctatttggga
    25321 tagtaaaaga cagagggaat tagttgcatt tattaggttt tattatattg aagatataga
    25381 tggaaataaa ataaaaagag ttgagtacta cacagaaaat gatgtaactt actttgttga
    25441 aagaggtaat agttttgttc aagaattttt atatgatgaa tatggaaaaa tgactgatgt
    25501 acaagaaggt cattttagaa taaataacaa agaacagggt tggggtaaag ttccatttat
    25561 accttttaaa aataatgaaa agtgtgtctc agatttaact ttctataaat cattaataga
    25621 tatatatgac aataatattt ctacactagc agataactta gatgaaatac aagaggttat
    25681 ttatgtatta aaagaatatc caggaacaag tctacaagag tttatagata atataagata
    25741 ctataaatca attaaagtag atggtggagg tggagttgat aaactagaga taaatatacc
    25801 agttgaagct aaaaaggagc ttcttgatag attggaaaag aatataatta tctttggtca
    25861 aggagttaat ccagaatctc aaaacacagg tgacaaatcg ggtgtagcac ttaaattttt
    25921 atattcacta cttgacttaa aatgttctaa gactgaaaag aagtttaaaa aagcaattag
    25981 agagctttta tggtttgtgt gtgagtattt aaagataagt ggtagtaaga gctatgatta
    26041 taaaacagtt caaattactt ttaatcactc tatgataata aatgaagctg aaaagataga
    26101 tatggcagct aaatcaactg gaattgtatc agatgaaact attgtttcta accatccttg
    26161 ggtcgaggat gttaatgacg aacttgaaag acttaaaaaa caggaagata ctcaaaaaga
    26221 gtatgatgat ttaattccta ataatcaaga tggtgttata gatgaaacat aaagattatt
    26281 ggaggaagag atttgaacaa ttagaagaag ctcagaataa taaaagtata aaatattatc
    26341 ttgaattaga aaaacaatat aaactagcaa tgtctaatat agaaagagat atacttatat
    26401 ggtataacag attcactgaa aatgagggaa tatctttatt ggaagctaag aaactgctaa
    26461 atacaagaga actagaagag tttaaatgga gtgtagaaga atatattaaa tatggtaaag
    26521 aaaatgctat aaatcaaaag tggatgaaag agttagaaaa tgctagtgca agagttcata
    26581 taacaaggct tgaagcttta aaactgcaaa tacagcaaca agtagaagtt ttatatggaa
    26641 atgaacttga tggtattgat aaactaatga gagatattta tacaagtgga tactatcata
    26701 cagcttttaa tgttcaacaa ggagtaaacg ttggttggag tttaatgagt cttgatacta
    26761 acagaataaa taaagttatt tctaagcctt ggactagtga tggattaaac ttcagtgaaa
    26821 ggatttgggg taagcataga cctgctttag taaatgaatt acatactaag ctaactcaat
    26881 caattattag aggtgaaaat ccaaagaagc tagtaaatga ctttgctaag agatttaagg
    26941 tatctaagtc acaagctaag aacttaataa tgactgaatc agctttcttt gcatcagctt
    27001 caagaaaaga ttgttttaat gatttagatg tagagaaata tgagattatt gctacattag
    27061 atttaagaac ttcaaatata tgcagagagt tagatggaaa aatatttgat atgaaagatt
    27121 atcaagttgg aataacagct ccaccatttc attgtcgttg taggacaaca acagctcctt
    27181 ggttcgagga tgaagaaggc tatagagcag caagaggaga agatggaaaa acatattatg
    27241 taccatctag tatgaagtat aatgagtggt atgagaagta tgttaaaaat aatagtaaac
    27301 aaactggtgc aaaatatact aaaggtgata tcgagtggaa tataagaaga gaagaagaag
    27361 cagaactata ttacgataat attagaaata gaaaagatga tatttccaaa atatcaaaga
    27421 atacaaattg gtcagaaaaa agtataggtc aaattaaaaa tcatattttc tacaatactc
    27481 atataatgag agatggaact agacgtatgt tggattctga ctatagtatg tcagttgctt
    27541 ggcaaagact tataaatggt acatacgaag atattgatat tctcttatta aaacatgaat
    27601 accttgaaag tatatttgag aaaaagtata atataagtaa cttagaagcc catagaatga
    27661 ctgagaaaaa gcatgattgg tataaagaat taattaaaca gaaaggagag tttgaagaag
    27721 atgattgtct taatgaactt attagaaaag aataatgaat atgttatata tagttatgga
    27781 tatgaagaaa ataagcttga tggaagaata aaaatatatt tagatgattt ttataattat
    27841 gaaataataa aagagtcaaa agatgaacat ataagtaaat cagcaacgtt aaaagctatt
    27901 tctaaactta taaaagctgc taaaaataac gatttgaaaa aagaaatgag ttatcaatgt
    27961 tagaagcact tactgaacaa taaattagta ggtgctttta ttatgtaaaa gtttaaaaaa
    28021 gtaggtaatt ttaatgtaaa tattaactca agttatagct gtagtttgtg tagtacaaat
    28081 ctttattaat tgtattgcta atgtcaatgt aggtattctt tgcaataaat taaaagaaaa
    28141 aaatgaagct aatatagata aagtttctga tgaaattcta aagagagtag gagaagaatt
    28201 aaataaatca ctagacaaaa gtctttaaag acttttttta ttgtgtaaaa aatgaaagga
    28261 gatatttaaa agatggattg gttagaagaa ttgctagaag gaataaaaat agaaaataac
    28321 aaaattgatg tagtttctct tcaaaaatct atagaaaaga aaataaaaga gactacaatt
    28381 actcaagaag attatacaaa tcttgaaaca caacttaata cagctaatga agctattaaa
    28441 aagtttgaag gaggtatgac aaaagaagat gtagagaatc taaaaacaac ttatgaaact
    28501 gataagaaaa ctttggaaga aacctacaaa aaagaaattg aagaaaagga ctttaattac
    28561 tggttaaatg atgcttttaa gtctattaaa tgtagggatg aaatagcgtt aaaagctcat
    28621 ttagatatag aagcactaag aaatagtaaa gatagacaaa aagcttttga agagcaaata
    28681 aaccctttga aacaggataa agattatttg tttaatgcaa cactagaagg tgaagagcct
    28741 aaaatagata ctataacacc agggcaagag cctaagataa atgattttgg ttttaatttt
    28801 actggggtaa gacctcatga aaataataat aaataggagg aaataaaatg gcagcactaa
    28861 attatgcaaa agaatattca aatgttttag cacaagcata tccttatact ttaaacttcg
    28921 gggatttgta tgcaacacca aataatggaa gatatagatg gactggttct aaaacaatag
    28981 aaataccaac tatatctaca actggaagag tagattcaaa cagagataca atagcagtag
    29041 ctcaaagaaa ctatgataat gcttgggaac ctaaggtatt aactaatcaa aggaaatggt
    29101 caacattggt tcatccagca gatataaacc aaactaatta tgtggcttca ataggcaata
    29161 taacaaaagt atataatgag gaacaaaagt ttccagagat ggatgcttac tgtatatcta
    29221 aaatatatgc tgattggacc gcattaggta acacagcaga tacaactgtt cttacaacag
    29281 caaacgtatt agaagtattt gataagttaa tggaaaaaat gacagaagct agagtacctg
    29341 aaaatggaag aatattgtat gttactccag tagtaaatac acttatcaaa aatgcaaaag
    29401 agatacaaag aacagtaaat ataaaggatg caggaacttc tcttaatcgt caaacaactg
    29461 atattgatac agttaaaata attaaagttc catctaacct catgaaaact gcatatgatt
    29521 ttacaactgg atggaaagta ggagcaggag ctaaacaaat ctttatgtcc ttagttcacc
    29581 caagtgcaat aattacacct gtttcttatc agttctctaa gttagacgaa ccaacagcag
    29641 ttacagaggg aaaatacttc tactttgaag aaagttttga ggatgtattt atattaaata
    29701 aaaaagctga tgcaatacaa tttgttgttg aagcatagaa aaaggagtga tatatagtgc
    29761 cacaagtaaa aaaactaaat agaatactaa ccatagaaga atgtaaaata gatgatttct
    29821 tagagatggg atatgatttg atagatgaaa ctggtaaggc agtaaagtat ggcaagtcat
    29881 taagtgtaaa agatttaata gctgaaaata atattttaag gtcaaaagtt gagtctttag
    29941 aagaagaaaa taagcagctt aaagagaaaa ataaacttac taaaaagtag gtgaaaatta
    30001 tggaaaataa tataattgat gaaatagaaa aaagacttga aagttttgga tatatattaa
    30061 aagatggaga taagtggtta ataggttttg taagagaaaa aatagaaaat attattaaac
    30121 tagattgtaa tataaaaact atgccaattg aattgaaaga aattgaagtt gatatgatag
    30181 ttggagagtt cttatttacc aagaaaaata tgggtcaatt agatatagaa agcattaact
    30241 ttgaagctgt agaaaagtct atatcagaag gtgatacaaa ggtagatttt gctataggaa
    30301 gtggttctca aacaccagaa caacgctttg atagcttaat agcttatctt actacttatg
    30361 gcaagaataa gatattaacc tttaggtgct taagatggta agtaaaacta gaaaagcaat
    30421 agaaatgtta tatagagata aatgtactat agttgagcat cagccaatca aagaccctgt
    30481 aacaaaacga actaacaata aagaagtgat tgtattagaa aatcaaccat gtaaactttc
    30541 atataaaaat atagtttctg ctacagaagg gaaagtagct aagctagagc aaactattaa
    30601 actctttata tctccagata tagaaattaa agcaggttca aaacttatta taaatgataa
    30661 agagtatgta agaagtggag aatctgctat atatccaaat catcaagaaa taatacttga
    30721 gctatttaag gataaagcat aatggctaga tggggcagtg ttgattttag agagtttaaa
    30781 agagtttgta aaaagatgga gaagcttaca aagattgatt tagataagtt ttgcaaagat
    30841 gcagcaagag aattagcagc aagactctta ggaaaagtaa ttagaaggac accagttgat
    30901 acaggattct taagacaagg atggaatgga atggcttatg ctaggtcgct tcctgtgtat
    30961 aaacaaggaa ataattatat tatagaagtt gttaatccga ctgaatatgc aagttatgta
    31021 aatttcgggc atagaactaa agatggtaag ggatgggtta aaggacaaca tttcttaact
    31081 atttcagaaa tggaactaca aagtcaagtt gataagataa tagagaaaaa actattaata
    31141 ttgcttaaag gagtgtttga tgcttaataa tattatagat ggaatatcag taaagttaga
    31201 taaatcgttt ggagaaaaat atacaattta tagcgaagat gtggagcaag gtattaatga
    31261 accttgtttt tttattgttc ctttaaatcc aagcaagaca ccatatccaa gcgggagaga
    31321 attaaagaaa aattcttttg atgtacatta tttccctcat tcagaagata agaattttga
    31381 aataaatgag atagctgaga tgctactgga ggaattagag tatatagaaa ttgatggaga
    31441 tttagtcaga ggtacaaata tgaattttga aattatagac aatgttcttc acttctttgt
    31501 tgattataac tattttacta taaaaaataa tgataccaat aagatggata cagtagagtt
    31561 attcggtggt ttgaagagag gtgataattt tgagtaaagc attaagcaaa gaagataact
    31621 acaagtttac taaggagcag atagttaact ctaagaagta tgtaaataga aaagatttat
    31681 taaatgcaat tttaaaagaa aatgagttat attccttctc agaggtagag gatagaataa
    31741 ataaatttat gaaaggagtg agttagatgg ctttaggtgg aggaacattc gtaacgcaaa
    31801 ataaaatact tcctggtgca tatataaatt ttatctcagc taagagggca accagttcat
    31861 tatcagatag aggtattgtt gcaatgcctt tagagttaga ttggggcata gatgaagaag
    31921 tattccaagt aaccagtgat gattttgaga agtattcagt gaagtatttt ggatatgatt
    31981 atactcatga gaagctgaaa ggtttgagag atttattcaa aaatataagg ttgggatatt
    32041 tttataaatt aaataaaggc gttaaagcca gttgtactat agccacagca aaatatagtg
    32101 gtatcagagg aaatgactta aaagtaacag ttacaacaaa tatagatgat aatactaaat
    32161 ttgatgttgt aactttgcta gataataaga aggtagatac tcaaatagca aaggttatta
    32221 cagacttgca ggacaatgac tatgtaattt ggaagaagga tgcaacacta gaagcaagtg
    32281 caggacttgt atttactggt ggaactaatg gcgaagctgt gacaggagca gagtaccaag
    32341 ctttcttgga taaaatagaa agctatagct ttaatgcttt aggatgtttg gctacaacaa
    32401 cagaaattaa aagtttattt gtagagttta caaagagaat gagagataag gtaggagcta
    32461 agtttcaaac agtactatat aagaaaagtg atgcagatta tgaaggtgta gtgtctgtag
    32521 aaaataagat taaagatact gaattattag aatctagctt aatttactgg actactggag
    32581 ctatagcagg atgcgatata aataaatcta atactaataa aacgtatgat ggtgagtttg
    32641 atgttgatgt aaattacaca caaatacaac ttgaagaagc tttaaaaact ggtaaattta
    32701 tattccataa agttggtgat gaagttcatg tgttagagga tataaatact tttgttagtt
    32761 ttacagatga taaaaatgac gatttttcaa gtaaccaaag tgttagagta cttgaccaaa
    32821 ttgctaatga tattgcaact ttatttaatg aaaagtattt aggtaaagtt ccgaatgata
    32881 aggcaggaag aataagtttc tggaatgatg ttgttaaaca ccataaagaa ttagagaata
    32941 taagggcaat agaagatttt aaaactgatg atgttagtgt agagcttgga aatgataaga
    33001 aaactgtcat agtatctgat gctgttaagg ttataaatgc tatgagtaag ctttatatga
    33061 cagtttcagt tagttagaga ggggagtgat aatatggctc aaacaataaa tgctaaagat
    33121 acagttagtg caaagaaagc tgaatgtttt ataactatag aaggcaaaag atataatttt
    33181 atgcaagcta tagatttaga ggctaaaatg gaaaaaaata aaagtgaagt tccaattcta
    33241 ggaagaacaa caaagggaaa taaaacaact gggagtacaa atactggaag tgcaacattt
    33301 cattataata cttctatttt tagagaatta ctttacagat ataaagaaac tggtgaggat
    33361 atttattttg acatacaagt tacaaatgaa gaccctacat ctgctgtagg aagacagaca
    33421 gtagtactta aagattgtaa tatggacagt ggaataatta ctaaatttga tgctgatggt
    33481 gagtatttag atgaagatat ggatttcact tttgaggatt gggaattagt agaaaaattt
    33541 aatttattgg caggaatgga gtaaaataca catttataaa ttatatatgt gtatttttta
    33601 tatgaaaaat taaaataaaa ggagattaga ataatatgag taatttaagt gcttttttaa
    33661 gtcaaaatgc aataaaggtt gataatgtaa aatatgtagc gagtaacaga tttttagata
    33721 aagaagggaa accagttgaa tgggaattaa aagttttatc atctgaagaa gacgaagcac
    33781 taagaagaaa gtgtactaaa agagtaaaag tgattggtaa caatggtaag catactggac
    33841 aatatacaag tgaaattgac tacaatagtt atgtagctga attatgtgta gcatctacag
    33901 tatttccaga tttaaaggat gccgaactcc aaaatagtta tggagtaatg ggagaagctc
    33961 agttattaaa gacaatgctt acagcaggtg agtatgtcaa ttatacagta aaagtgaatg
    34021 aagtcaatgg atttgataca tcttttgagg ataaagtaga agaagcaaaa aactaatcag
    34081 aggtggcgat tttgatgcta gcatcactca ttattgtatt caaaaattaa agtggaagcc
    34141 aagtgaatat atgaatttag aagttaatga gagagcgtta gcagccgcct caatacttat
    34201 aaagatagaa gatgaagagg aagcaatgaa agaagctgaa agagagagaa agaggggacg
    34261 aagaagatag caaaataaaa aaataaatat agaataggta aaatatgtaa taattatatg
    34321 ttataatatt tttagcaaga agatgtaatc tacaatttat agagtggagt tcatactggg
    34381 ataaaaccta cttcctaatg aaaggaggtg ggaagtatga ataacttttt acttaatgta
    34441 atagctggcg ttattgctag tttaatattt tgcataattt gtaaagtatt tctaaaagta
    34501 aaaagccact caactcgtgg caagagtaaa agtggctggg aatttgattt taaaatcaag
    34561 ttccataagt tcaaatagat tcatttaatt atgaacttca ctctaccgca aaatagattg
    34621 tagttcttct tgcttttatt ataccacaaa ttagaaaaaa tattgtttat ataaaataaa
    34681 aaataaaaat ttttattaaa aaattgaaaa cttgattata aagcaattaa tttataaaat
    34741 atatataaat aagtaggtat ttatttactt gaatttcatt gtttatataa aaaaaatgga
    34801 aaaatatgta ataattatat gttataataa ttgtagcaag gataataatc gaaagtgcga
    34861 agggtgatta ttttcatatt aaacgccaaa ttccaaataa ggaaggaggt gaaattatat
    34921 gataggtttt ttattaagca tactagctgg tgttatatca gcttatattt atgacaaaat
    34981 aaaaaatcac ccagacgcca ataagggtga tttaaaaaaa taattcttta aatcaatttt
    35041 gatggaaata gctactcttg tataaagtaa attatttcct tgcttttatt ataccacaaa
    35101 ttggtacaga tattcaaaaa taatattttt atgatataat aaaaatgtag agattttgca
    35161 gtgagcgata tttgttacaa aatatggctt aacacttgaa atctaagatg ttgagggtgt
    35221 gtgataaatg ttatcaattg cactactcat ggttcactgc aaatttgaga gagatgcgta
    35281 tgtgtaggta ttggaaatgc caagtttatt ttggggtttt agattaacta tatggaatgt
    35341 aaattgtggt ggtattttta agtcctaagc attttcgctt ggttttagat taactatatg
    35401 gaatgtaaat gtcggagaag ttgctaggta attattgtat ccattttcgt tttagattaa
    35461 ctatatggaa tgtaaatttc ctcccgcttc cgctgcacgt aaaactggtt ctaggtttta
    35521 gattaactat atggaatgta agtcgtgatt ttataaaaga tttatatagt atgtttttag
    35581 ttttagattg actatatgga atgtaaattt aatagaacct agtaaaacgg ctacactacc
    35641 tttagagttt tagattaact atatggaatg taaattatct tcgctgtccc aaataaacat
    35701 cataaccaac aagttttagc tgcttctata gatagtttta gattaactat atggaatgta
    35761 aattaactaa atgttcttgc agtgtcacta catgtttgag ttttagatta actatatgga
    35821 atgtaaataa aaaatcgtta agattatttt cttcttgacc aaattgtttt agattaacta
    35881 tatggaatgt aaattcataa taatactctt tgactgcata ccaaacatgt tttagattaa
    35941 ctatatggaa tgtaaatcca gtcatgaaac caaaagaatt tagaccagat tcagttttag
    36001 attaactata tggaatgtaa atttttcatc aagtatattt attgttaatt ggtctggaaa
    36061 gttttagatt aactatatgg aatgtaaatt tcgcaactta tgatggtgaa atgattacat
    36121 taacaggttt tatattaact atgtggtatg taaattaatg ttcttttctc tatactgctc
    36181 atgtttacat aggttttata ttaactatgt ggtatgtaaa tgtgcaaaac atttagaagc
    36241 aaaagaagta aaaataagtt ttagattaac tatatggaat gtaaattaaa ttgttgaaat
    36301 ttctatatcg acgcttaata aatcgtttta gattaactat gtggagaaaa actaaataaa
    36361 caaagaaagc acttacaaat atgtaggtgc ttttattttg ctcaaatttg gtcggttggg
    36421 taaaataatt agaaaaaatt gggataagtt attgactttc gagttccaaa actatataat
    36481 ttaattatgg aactcaaaag tgaggtgata acatgagtcc taaaaaaata ggaagacctg
    36541 ttgtaggaag ccctaaaact aatgatatta aggttagagt agatgatgaa actaatgaaa
    36601 aattagatga atactgtaaa aaaaataacc ttacaaaagc agaggctata agacagggaa
    36661 tccatttact tttagagaaa taacaaaata gagcattctc cccgaccaaa gattgaatgc
    36721 tctatttcca acaaagaagt tagcacttct atatgaaata ttttatcata tagagaaact
    36781 tctttcaaat caatttgagg aggaatttat atgaaaacta atttagaatt aatcaaatca
    36841 gaaaactttg gagaagtaga atgtaatttt tatgaaaatg atactaaaga tatattaatg
    36901 actagagaac aaataggaat ggctttagag tatacagaac caagaatagc tataagtaaa
    36961 atacatgaac gtaacaaaga tagacttgat aaattttcaa ctgtagtcaa gttggttaca
    37021 gttgaaggga atagagaagt attaagagat actatagttt ataatacaaa aggagtaatg
    37081 gagatatgtc gttttagtag acagccaaag gctgatactt ttatggattg ggtttgggat
    37141 gtagtagaat ctatattcaa gactggtgca tacataacta acaatgctaa tcccgaaaaa
    37201 ctaagagaaa aagcaagtga gattgagaaa ttacagttag cttataacag cacatctatg
    37261 ttaaaagaac tattagatgg tgcaggcttt gacaacaaat ctaaactatt aacagcaaag
    37321 acattgtaca agaaagcagg cattgattta cctatagaaa ttgaagaaga ggaatctttc
    37381 tttgacacaa aacaaatagc atctaaactg aaaatatatt ctaagagtaa taaaccagca
    37441 cagttggctg tttgtgagat tattaaaaag attgatttag aagaaaatga agtaaaagga
    37501 gtttgggaaa ctaatggaag ttggactggt actgtaaata aatatacaaa gagtgtaata
    37561 gataaaataa gaaattggat agaggaaaat aatagacctg ctaagattgc aggtgagaag
    37621 aagaatttcc atgtagtgta caaaattgag taaatttgat tgtattaaat aatttagttt
    37681 agtttaattt tgagggggat taatacaatg tatgagaatt tacttgatat agatagaata
    37741 gaacttatca aagaacttgg aaatatcttt gaaaaaatga aaaatgaaaa tccggatgaa
    37801 ttttatagat ttgtaagttt agtgaaagaa gagtgtagga aaaaagaaga gaaaaataaa
    37861 taaatagaca aataaagcac ttgaatattt tactgtttca agtgctttat gtagtaaaaa
    37921 atgatataat ataggtagga gttatattaa ctaagtggta tgtaaatgct gtatcctttg
    37981 ccccttttgc tgcgtcactc aaggttttta tattaacaat gtggtatgta aagtcgttta
    38041 tcattgctag cctcgcattc gtactggtca attttatatt aactaagtgg tatgtaaaga
    38101 ttcgcgatac tatttgtgca gtatctaagt aggatggttt tatattaact aagtggtatg
    38161 taaatcattc actacaaaat atatattcta atgtatcatt cgttttatat taactatatg
    38221 gtatgtaaac aatatatata gcaaatctgt tatattaact atgaagcgtt ttatattaac
    38281 tatgtggact taaaattaaa aataattaaa aaacacttac ttaggtaggt gtttttttaa
    38341 ttgaaagtag gtgattataa tgtaaaaatt ttactgatat agtataataa tcttataaaa
    38401 ttgcgtaggg ggtaatatta tgggattatt cggaagtaaa gataattgtt gtatttgtgg
    38461 agaaaaagga aaacaaaaaa tagctgatgg atggttgtgt aaagagtgtt ttaagaaata
    38521 tgcagttgcc acctttactc caggaaatac tttatatcgt gggttaccaa ctaaattaga
    38581 ggtagagaag gctattgaat caaaagatga taaagaaaaa gagcttaaaa attttaatcc
    38641 tacaaaaaaa atattaaaat ttatggaatt tgatgataat aataagaaat ttattgtttt
    38701 aaatgggttt aatagagaaa aagtgaattt aagtgtttat aattacagtg atgttataga
    38761 atatgaactt ctagaaaatg gcgaaactgt gactaaagga gggataggaa gagctttagc
    38821 agggggagct ttatttggag gagtaggtgc tgttgttgga ggcgtaacag ctaaaagaac
    38881 aacaaaagct ttcatagata gtcttaagat aaaaataaca ttaaataatt taagtaatcc
    38941 taatgtttat gtaaatctga tacaattaag aactaaaagt aattcttcaa tttacaaaat
    39001 ggcatattct tctgcacaag aaatattatc tgttttagca ataattgtaa aggataatga
    39061 agctgttaat atacaaaata attctaatga tgcaatacaa caagtaaagg gattaaaaga
    39121 attgttagac ttaggggcaa taacagaaga agaatttaat actaagaaaa aagaactatt
    39181 aaatctttaa acacttacta atgtaggtgt ttttttatat ggaaatttat gaaaggagag
    39241 tgaggaaatg gctacaatac aaacttcaat aaagattttc gacggaatga cacctgcatt
    39301 tcgcaatatg actacatcta ttaacaccac gattaatagc ttggagaggt tacaacaaag
    39361 gttgcacaat ccacttaatg ctggcagcat acaggcatct caacaaagcc taaataatat
    39421 agaaagtatt ctcacaagga tagaacagaa aattggaaga aacacaaacg aacaagaaaa
    39481 ctttaataat aaaataagac aaggaagtga agcaggctct ctattagtgt ctaaattaaa
    39541 aagtatcgct gggatataca ttggaataaa aggaatagaa agtattacaa aagcaacaga
    39601 tacaattgca agtacaaaag cacgtttaaa tctaatgaac gacggattac agacaacaga
    39661 acaattaaac aagatgattt atttgtctgc tcaaagtgct agggctagtt atgcagatac
    39721 ggcagcacaa gtcgctaaac ttggaatatt agcaggagat gcttttggaa gttcggcaga
    39781 ggtgataaag tttacagaac ttatgaataa agcttttgta attggaggaa catcagcaaa
    39841 tgaagctagt gcggctatgt atcagcttac tcaagctatg ggtgcaggga aacttcaagg
    39901 tgatgaattt cgctctataa tggaaaatgc accattgcta gctgctaaaa tagctgatgc
    39961 aatgggaaaa actaaagacc aattaaagga attgtcaagt agtggagcaa taacagcaga
    40021 tgttataaga aatgcactat ttaaagcttc taatgagata gaaaagaaat tcgcaagtat
    40081 gccaatcact ttttctcaag ctcttacaat gatgaaaaat gatgcttata tgatattttc
    40141 tgagactctt ggtaagataa gtggagcatt acaaagtgtt agatttagcg agattgttgt
    40201 atctatgcgg aatgttatga ttgcaatatc ttcaaatatt tatgatacat taaatattat
    40261 aaaaaatata ttaaatagtg attttttctc agattttgtt caaggaatta cattaggagc
    40321 agtcttaata atacaagggc ttggaggaat tactaatgct gcacttagcg ttgttaatgt
    40381 ttttgcacag aactggaaca taatagagcc gattgtttat ggggtggcgg cagcgattgg
    40441 tatatataca atagcagcta tagcacttgc agtagcaaat aaagtagcat ctttatcagc
    40501 tgcttggttt aattttcaga tgactcaaac agtaattatg cacgaattag ccacaggagc
    40561 tacatggatg caagtagcag cacaatatgg attaaatgca gctttatatg cttgtccact
    40621 tacatggata gtgcttggat ttatagcagt aatagctgtg gtttttgttg cagtagctgc
    40681 aataaataaa ttcgcaggaa caagtttgac tgtcttagga gtaattgtgg gtgcagtatt
    40741 tgcagcagtg gcaacaatac aaaatataat gattgggctt ctcaatggat gtatagctgt
    40801 aaatgaaggc attacaaatg gttggaatca gtgtgtatat ttgatgaaac aagcaattgc
    40861 caaaggtgta atctttataa tcgagaaaat ggcatctcta aataattctg taaataatgc
    40921 tggaaatgca cttgggaaag ctttcataga tggggcaaat atcgcaatac gaggtgtaaa
    40981 caaattaatt gacctaataa ataaaatacc agggataaat attggtaaag ttggagaagc
    41041 aacatttacg cctgtcaagg cagataatag ttacatcaaa caacagattg acagtttaaa
    41101 taaatgggta ggagatgcac cagaaaaaat aaaattggat agaatgggat ataaagatat
    41161 tggagcagaa tttcaaaaag gaaatgcact tggaactaaa tggcaaaatg ctataactga
    41221 taaatttaaa gatacttttg acattaataa gatgctagaa gatgcaaaga aaaaattagg
    41281 attagacgat ttgtggaata aacaaaatcc tttaaacaac cttggtggat ttggtggaga
    41341 tttaggaaaa aatgtaaagg acacggcagg caatactgca aagatggcaa agactatgga
    41401 taaaagtcaa gaggacttaa aatacttaag agatatagca gagcaagaga ctatcaatcg
    41461 attcacagga gtaaacatta aaattgatat gaacaataca aacaacataa gtaaagatgc
    41521 agatgttgat ggaatagtaa atgtcttaac tgaaaaatta aatgatgcca tggttgtttc
    41581 agccgaagga atagtttagg aaggagagtg agaaaatggc ttatgatttt tacctagatg
    41641 gagtacaact accaatcgca ccacccaagc ttgaagtcaa agtgacaaat aaaaacaaga
    41701 cagttgattt aataaatgtt ggagaagtaa atatattaaa aaaagaagga ctatctgaga
    41761 taagttttga agcagaattt acacataata aattaccttt ttatcgtgga acttttaggg
    41821 atgttcaatt ctttttaagt aaactagaat tactaaaaac tgattgtaag ccatttcaat
    41881 ttattgtctc gagggaaatg ggtggaaaag tactatttaa cactaatata aaagtatctc
    41941 ttgaagagta tgctatttca gaagacgcag ataatggttc agatacaaaa gttgcaataa
    42001 agttaaagca atatagagat tattcaacta aaaagttagt tcctgctcaa cctaaaacaa
    42061 ctaatgagac tagcagacct aatgtaaaaa tagagcctaa aagggtcgat tcagtcaatg
    42121 caccaaatgc taaaacatac acagtcaagg caggggatag tctttggtca atttgtcaga
    42181 aacaacttgg aaatggttca ctatataaga aagtgtatga gttaaataaa actatgatgg
    42241 ataaggctaa taaaggtaaa aatctatcta agtatactat ttataaaggg caggtgttga
    42301 ggcttgtctg atgatttagt tttggcaaat gatagggata taaggctggt aatagcacat
    42361 tgggaagatt tctatgaacc tgctgtcatt gatggagtta catgggaaat agaaaggcga
    42421 ggtacacctt ctaaacttga atttacaatc gtcatggatg atatactaca gttttgtgaa
    42481 ggtaattcag ttcgtttata ttacaagggt acaggtgtat tttatggata tatatttcaa
    42541 aagaaaagag ataaagaaaa tcacattaaa attgttgctt acgaccagtt gagatatttt
    42601 aagaataaag atacttatgt atatagtaat aaaactgcaa gtgaacttgt aaaaatgttg
    42661 gctaaagatt ttaatttaaa atacaatgtc atagaagata ctaagtataa aatatctagg
    42721 atagaagaaa ataaaacact ctttgacatg atactaacag cactagatga tactctaaga
    42781 gagaaaaaag aaatgtatgt tttatatgat gattttggaa gaataacatt aaagaatgtt
    42841 gcatcaatga aactggatac tgtcatgaac aatgatgtca tagaggactt tgactataat
    42901 tcaagtatag atagtgatac ttatacaaaa atcaaacttg taagagacaa cgaggagtca
    42961 ggaaaaagag atgtgtatat tgctcaagac tcaacgcata tgaggagttg gggaatactt
    43021 caaatgtttg atacagtaga caaaaacatg agtgaagcag agataaaaca aaagtgtgat
    43081 atacttctaa aactatataa taagaaaact aagtcattaa gtttaaaaaa tgcatttgga
    43141 gatattagag tgagagcagg ttgtttagta cctgtttttt taaatctagg agatattaaa
    43201 cttcaaaatt atatgctagt tgagaaagta aaacatactt ttgaaaataa cagtcacttt
    43261 atggatttga ctcttgttga tggagatgaa tttgcttctt attcttcaag ctcatattca
    43321 agtggaaata ctaacaataa ggatgaaaag aaaaatggtc ctgcacaaag tattacgaaa
    43381 aaaaatacag gtaaaaaagt tcctgctata tttactgcat attatccagg gaacaatgca
    43441 atggaaggcg gaaaaacaga ttgcaatgga aagccacttg atgtaaaatc aagaactgtt
    43501 gctggtccaa tgaatcgaga aggagttaag aaaacttggt atactgatga ttttctaaat
    43561 aaacatccag tttttgaata tggagataaa gtaaaaatta tacttcctgg tactgcctat
    43621 gacaacaaag tatatacagt taaagataat ggaggaagaa tatatgttga aacaaacgga
    43681 acatatcata tagatatact attagctaat gctagtgaat gtaaaaaatt tggtagaaag
    43741 aatggctata taattatagg tggagatgaa gaacaaacat atcaagttga aggtaataac
    43801 caaagtaata caaataacaa ttctaaagaa gataaattaa ttagtatagc aaaaagtaaa
    43861 ctgggttgta attatgtgta tggagcagaa ggtcctaata attttgattg cagtgggttt
    43921 actcaatggt gttataaaca aataggtata aaaattcctc gtactgcttc tgcacaaagt
    43981 aaagcaggaa aagcagtaga tttaaaagat agaagcaagt ggaaagcagg agacttatta
    44041 tgtagaattg gtggaggaag tagtaatcat gttgtaatgt acattggaaa caatcaaata
    44101 attcattcac cacaaacagg agatgtggta aaaatagagt ctgttaattc ttatagaaaa
    44161 ggaaaagcat acacacatgt gagaagattt atataagtga ggtggcaata tgagccaaga
    44221 tttattacag ataataaaaa aagctgcaat ggatgcagta gaaacaagca acccaatgag
    44281 ggttgtattt ggaacaatag aaagtattag tcctctaaga gttaagatag aacaaaaact
    44341 atctattggt gaaatttttc taatacaaac agatacattt aaaagatata cagataaaaa
    44401 aataggagat aaattagtct taattcgtat gcaaggaggg caacaatact tgattttaga
    44461 taggatgtga taaagtgtta ccaagtgata atttagatta tgatattgaa gatgtatcaa
    44521 taattaattt tgatgtaaga caagagccaa gtaagacatt taaacttcat atagaaaagt
    44581 ctaagattga tggtatttgt gatgatgtag aagcattaaa acagaccatc tttttgattt
    44641 taaacacaga gagataccaa catctaatat atagttggaa ttatggagtc gagttgaacg
    44701 accttattgg agagcctata tcctttgtaa taccagagtt agagagaaga atcaaagaag
    44761 cactaattca agatgatagg gttgaaaatg tagataattt tgagtttgaa aatataaagg
    44821 gtaaagtaca ttgtaagttc acagtttaca gtaaatatgg aaatataaaa gcagagaagg
    44881 tggtgagtgt ataattgttt gagttaatga catttgaaaa tataattaaa agaatgttag
    44941 atagtgtacc agatactttt gataaaaggg aaggttctat aatatataat gctcttgctc
    45001 ctgttgctat agaacttaca gaaacataca ttgcaatgga tgaattacta gaccaaacat
    45061 tcgtagatac tgctagttat tattacttgg agaaaagatg taaagaacga ggaattacac
    45121 cactatctgc aactaatacc attgcaaaag gagtgtttaa tatagatatt ccacttgatt
    45181 ctaggtttaa tctaggagaa tacaattata ttgcaattga gagaatatct gaaaaaacat
    45241 ataaaatgaa atgtgaaact gctggaccta tatttgagtt aggaaaacta atacctattg
    45301 aatatataga tggtcttgaa actgctgaac taactgaaat cttgataaat ggagaggatg
    45361 aagagtcaga agatagttta agacaaagat attatgatag cctaaattca cagagctttg
    45421 gtgggaatat acaaaactat aaagatgaag ttaacaaaat acaagatgtt ggaggagtta
    45481 aggtttatcc tgtgtgggac ggtggaggaa ctgttaagtt agtaataatt aactctaatt
    45541 tcaaagtacc atcagaggat ttagttaatt tagtgcaaga agaaattgac ccaattggac
    45601 atcaaggaca aggcttagga ttagcaccaa tagggcataa agttactgtt acaggtgttg
    45661 taagtacaac tataaatata tcagcagaga taacatacaa aaatggctac acttgggaga
    45721 atataaaatc aattgcagaa gaagcaatag acgactattt aaatgaactt aacatgagtt
    45781 gggaagatga agaaaactta atagtccgta tatctcaaat tgaaactaga ttacttagta
    45841 ttgatggagt attagacatt gcaaatacaa tgataaatga ggttaaatct aatctaacaa
    45901 taaatagtaa cagtatagta gtgagaggtg aggtagttgg ataaagagat taatctaata
    45961 aattacttac cacaaattct acaagataaa gaagaatata taaaagtatt taatgtagaa
    46021 aataaagaaa taaaaacact acatgataaa ttaaaggacc tatcaaatga ccagttttta
    46081 gaggacctaa ctataagtgg tataaaaaga tgggaaaaga taatgtctat aacttctaaa
    46141 agtaatgaga gtttagaaga tagaaggttt aggattttta gtaaatatat aagtaaacta
    46201 ccttactcag agagattttt aaggaactgg ctagataatg tagttggaga aggcaattat
    46261 gagttaacta taaataatgc tacttataat atacatcttg aaagtgatgc tagaaatcaa
    46321 gattggtttg aggaagttca ttcttttgta agtaatatta agccatgtaa tatgacttta
    46381 gattacacta gagtgcttat aagcaaagac aattatatga attttggtat aacaacccta
    46441 atgggtcaag aaataactat atacccttgg agtccaccag atatagaaac ttatggagaa
    46501 attgatgtat taactggcaa tggagttgga taccaagaga taacaatatt ttaggaggtg
    46561 atatattggc tatagataaa agttattaca ctataattac agatgtagga aaagcaaaga
    46621 tagcaaatgc aagtgtcaca ggtaataaag tgggatttgt aaaaattcaa cttggtgatg
    46681 gaggagggag tgaatatact ccaactgaga gtcagacagc tctcaaaaac gtggtatggg
    46741 aaggcaatat tggaaataca actacagatg aaactgcacc aaattgtata atattagaga
    46801 gtttaatacc atcaagtgta ggcggattta tgataagaga aataggatat ttagatgatg
    46861 aaaataattt aattgccatt tctaaataca aagagtgtta taaaccttct atagaacaag
    46921 gtgcagtggt agacatgaag gttaaaactg tgcttattgt atctaatgta aataatatag
    46981 aacttaaaat tgacccaaca ataatctttg caacactcaa agatatacaa gacttagaaa
    47041 ctaaaatagg tactgttaat actaaaatag atacaactaa aacagaatta acaaataatt
    47101 tagaaactgc taaaacagag ttaaatacta gaattgacac agaaaatgag aaacaaaata
    47161 ttaaaattga ccaattaatc gcaggtggtt caaatgtggc atctactcaa ataataacaa
    47221 ttgatgattg ggttgaggat gcagaaaatg gattcaaagc aactgtaaca catagtttat
    47281 taacacagag aatagttgta aatattatag atgctactac aaaagaaaat gtagttacaa
    47341 actttaaaat tatagatgat aattctatag aaattagaag tgaagcaagg tcagaattaa
    47401 acgtttatgt gataaatgga aatgcagaaa ctcattttat taatgcaact gtggatgata
    47461 acagagtgtc tgaaatgact acttattcgt ctaaaaagat acatgaagaa attagtaagg
    47521 tagcagagca attaacagga attaatagta atattatatc aacagtaaat aatgacatat
    47581 tacctatata gaaaggagag tgaggaaatg gcaacatcaa tgaataactt taatttacct
    47641 gatatgagaa aatatactaa aaatattgcg tttgacccat ttgcaggggg gaggcaaagt
    47701 tttgattggg gtggtaataa ccatggtttg caaggtagct ctaaagacac ttgtttaagt
    47761 tgtacttaca atggttcaac aattaattgg ggctcaggaa atgtttgggt tttgggagga
    47821 tatggtcaat atacatttac ttataattgt gaatcaatgc atgtggatac acaacaaaaa
    47881 tttccatata cttctaatag aataataact ataaaaggta gaccagtaat atctggttca
    47941 gatacttcac ttgggaataa aagaaaagga tttagtgtag attttactgt aagtgatgat
    48001 actccagatg ttaatttgat agtgcgtgtt tatcttgatg ataaattaat acaaaacatt
    48061 actcctgttg tacaaaattc aactttaaca gcaacagtaa cagatagtca actaaactct
    48121 ttatccatag atggaaatca caaactaaaa atacaattaa atgatggata tgataatttt
    48181 gatagaattt ttacttttaa aaaaatagaa aagggaatta atatatcaac gtcattagtg
    48241 actgatagtc aagctaagtt tactgtaaca aaaacataca gtgaattgac aaaaatagaa
    48301 tggtatttag atgaagtatt aaaagatact tttactacag acttgtattc agaaaaaact
    48361 attaactatg aacttacaga taatgcaata cacacattaa gaatagttgc tacagatgca
    48421 gaaaatatag tagaggaaaa agtaataagt ataagtaaga atataatgcc attacaacct
    48481 aatgctagtt taagtgatat atcaactaag ttaacagaga ttgggcaagg agtcagaaat
    48541 ggtaaaacaa gtattataaa cactttagca ttaaagaata tagaagcaag tttaaataat
    48601 acgctagtag agttatcaga gaaaataaag cagtcttttg atagtggaga cgctagttta
    48661 caggatttaa caagcaaatt aacacaagct aataatacta taagtcaatt aaatacaaaa
    48721 tataaatatg cgtcagggag tgctgcaaca aaaatgaatg cagcagatac tgcttattta
    48781 tatgatactg gtgtgtacaa cccagattat ccacaaaaac ctattgactg gcttaatatt
    48841 aaaggtctta attttattcc aaatgtattt tttgctgaat gtgaatgtaa acttaatagc
    48901 ccgactaaat ttcttaaata tcttgttttt gctgtttgtt cgttaccatc aatttctagt
    48961 gataaagatt ttacagttac ggttttatta gacaaaacat atggaaatca aaaatatact
    49021 gctaatggtc aagtctatat aaataataag ggaaatattt atatgaataa tgaaggcatt
    49081 tacattcctg catacaatcc ttctttgtct tatactttgt acaattggca tgctataaaa
    49141 tttatataaa agaggtgata aaatgaatag agcaaataga ataatttacg accaaactgg
    49201 taaaatatta ttccaaacag gagaagcaac aggagatata ttagagcatg atacaataac
    49261 agaattacat tatattgatg ttggatacgg aaatatagac tatagtaaac agtatataga
    49321 atctataaat ccaataacaa aagaacttat tttaaaagat attccaatct atttaagcga
    49381 agaagaaaag agaatacaag agttagaaaa tcaattacta attgcagaaa atgaaaaggt
    49441 aggaggatta ttataatgaa tataaataat gttgtagtaa gaatattagc agagaggatt
    49501 ttaagtagag gcttaaatcc tttaaaaaat cgagaatttg aattagatga tgtaactaac
    49561 acagactata ggacagcagt tgaaaattac attatagaac atagtggagt agtagaagga
    49621 atagaaccaa cagcgtagta ggttcttttt ttattgaaag aaggtgacta aatgactttt
    49681 aaagagttag ttaataaagt tagaaatctt gtattagaag caaagaaagt aactatagaa
    49741 gatacagaga ataactttac aagtgataat gttgagggag cattgaaaga attggcaaca
    49801 gaggtaaatg gacagagagc aaaaggaatt acaatagcaa atgatttaat agatatgata
    49861 taagcgaggt gaaaatatga cggaaaaatt aactgataat gctagtttaa gagaacttat
    49921 gacaacacta caaggtgtac aaactgattt tcagaatggc aaaagtaata taacaactgt
    49981 gttggggagc ccatttttaa acacagataa attagatata actaaaacaa aaatagaaac
    50041 attaaaaagt gcattagtga ataatttaat atataaagga gtgtcagcaa gtacacaaag
    50101 ttcatttact gatttaatta atgcgataaa ttttatagtg cagtcaatat taattgttaa
    50161 atgcaagggt acaggaagaa tagaagaagt aaacaatccg tacgttattt ataatgacaa
    50221 acctaatatc aaaggttgtt taaggattaa aggaacttta gaagtttata aaaattgggc
    50281 tacttttgca agtgctagac tagaaataat atatggagaa aaaaaagaat atatttatct
    50341 ttcatgtgat gctactgcta gtggtcgttc tacgaatttt gaaaaagata taattgtaaa
    50401 taatgataca caattaaaaa tacaagtgtt attaacagat gttggaacgg ggaatagttc
    50461 tactgcaaaa gcttacacat cagtttctga cgttacactg ttaagatagg agattaggta
    50521 tgaaaaaaca attttattac gattctttag aggaaaaaga aaaaataatt aatgaaaata
    50581 acaatttata tgtaatagag ttatcagaaa cattaaaagg taaatatata attttgtcag
    50641 ataatccaat atacgaaaaa cttagttatg aagaattaga aaatgagttg ttaacaaacg
    50701 aattaaaagg cgaaatttta taa
//
LOCUS       DAFKMX010000004        48829 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFKMX010000004 DAFKMX010000000
VERSION     DAFKMX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.004"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            74..1270
                     /locus_tag="LZB08_000178"
     CDS             74..1270
                     /locus_tag="LZB08_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBM7313699.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAIAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1363..1887
                     /locus_tag="LZB08_000179"
     CDS             1363..1887
                     /locus_tag="LZB08_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBM7313700.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1912..2088
                     /gene="rpmF"
                     /locus_tag="LZB08_000180"
     CDS             1912..2088
                     /gene="rpmF"
                     /locus_tag="LZB08_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBM7313701.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2295..2852
                     /gene="fapR"
                     /locus_tag="LZB08_000181"
     CDS             2295..2852
                     /gene="fapR"
                     /locus_tag="LZB08_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBM7313702.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2863..3885
                     /gene="plsX"
                     /locus_tag="LZB08_000182"
     CDS             2863..3885
                     /gene="plsX"
                     /locus_tag="LZB08_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBM7313703.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3911..4897
                     /locus_tag="LZB08_000183"
     CDS             3911..4897
                     /locus_tag="LZB08_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBM7313704.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5006..5935
                     /gene="fabK"
                     /locus_tag="LZB08_000184"
     CDS             5006..5935
                     /gene="fabK"
                     /locus_tag="LZB08_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBM7313705.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5951..6901
                     /gene="fabD"
                     /locus_tag="LZB08_000185"
     CDS             5951..6901
                     /gene="fabD"
                     /locus_tag="LZB08_000185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBM7313706.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6901..7650
                     /gene="fabG"
                     /locus_tag="LZB08_000186"
     CDS             6901..7650
                     /gene="fabG"
                     /locus_tag="LZB08_000186"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBM7313707.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7704..7928
                     /gene="acpP"
                     /locus_tag="LZB08_000187"
     CDS             7704..7928
                     /gene="acpP"
                     /locus_tag="LZB08_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBM7313708.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7957..9195
                     /gene="fabF"
                     /locus_tag="LZB08_000188"
     CDS             7957..9195
                     /gene="fabF"
                     /locus_tag="LZB08_000188"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBM7313709.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9421..11139
                     /locus_tag="LZB08_000189"
     CDS             9421..11139
                     /locus_tag="LZB08_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7313710.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     QYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGKY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11170..13170
                     /locus_tag="LZB08_000190"
     CDS             11170..13170
                     /locus_tag="LZB08_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7313711.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEEKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13521..13766
                     /locus_tag="LZB08_000191"
     CDS             13521..13766
                     /locus_tag="LZB08_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBM7313712.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKRIECKTKPTSCPDQLSLALENYMNIK"
     gene            13801..14526
                     /locus_tag="LZB08_000192"
     CDS             13801..14526
                     /locus_tag="LZB08_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBM7313713.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14548..15861
                     /locus_tag="LZB08_000193"
     CDS             14548..15861
                     /locus_tag="LZB08_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7313714.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            16067..16606
                     /locus_tag="LZB08_000194"
     CDS             16067..16606
                     /locus_tag="LZB08_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7313715.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16695..18680
                     /locus_tag="LZB08_000195"
     CDS             16695..18680
                     /locus_tag="LZB08_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBM7313716.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18904..19872
                     /gene="spoIIIAA"
                     /locus_tag="LZB08_000196"
     CDS             18904..19872
                     /gene="spoIIIAA"
                     /locus_tag="LZB08_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBM7313717.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19862..20383
                     /locus_tag="LZB08_000197"
     CDS             19862..20383
                     /locus_tag="LZB08_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBM7313718.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20400..20597
                     /gene="spoIIIAC"
                     /locus_tag="LZB08_000198"
     CDS             20400..20597
                     /gene="spoIIIAC"
                     /locus_tag="LZB08_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBM7313719.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20613..20999
                     /gene="spoIIIAD"
                     /locus_tag="LZB08_000199"
     CDS             20613..20999
                     /gene="spoIIIAD"
                     /locus_tag="LZB08_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBM7313720.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21084..22289
                     /gene="spoIIIAE"
                     /locus_tag="LZB08_000200"
     CDS             21084..22289
                     /gene="spoIIIAE"
                     /locus_tag="LZB08_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBM7313721.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPIVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22349..22966
                     /locus_tag="LZB08_000201"
     CDS             22349..22966
                     /locus_tag="LZB08_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBM7313722.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22978..23532
                     /locus_tag="LZB08_000202"
     CDS             22978..23532
                     /locus_tag="LZB08_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBM7313723.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23559..24248
                     /locus_tag="LZB08_000203"
     CDS             23559..24248
                     /locus_tag="LZB08_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBM7313724.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKEFQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24358..24720
                     /locus_tag="LZB08_000204"
     CDS             24358..24720
                     /locus_tag="LZB08_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBM7313725.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24805..25311
                     /gene="nusB"
                     /locus_tag="LZB08_000205"
     CDS             24805..25311
                     /gene="nusB"
                     /locus_tag="LZB08_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBM7313726.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25295..26365
                     /locus_tag="LZB08_000206"
     CDS             25295..26365
                     /locus_tag="LZB08_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBM7313727.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26362..27567
                     /locus_tag="LZB08_000207"
     CDS             26362..27567
                     /locus_tag="LZB08_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBM7313728.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27569..27784
                     /gene="xseB"
                     /locus_tag="LZB08_000208"
     CDS             27569..27784
                     /gene="xseB"
                     /locus_tag="LZB08_000208"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBM7313729.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27787..28674
                     /locus_tag="LZB08_000209"
     CDS             27787..28674
                     /locus_tag="LZB08_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBM7313730.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28704..29141
                     /locus_tag="LZB08_000210"
     CDS             28704..29141
                     /locus_tag="LZB08_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBM7313731.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29175..31040
                     /locus_tag="LZB08_000211"
     CDS             29175..31040
                     /locus_tag="LZB08_000211"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBM7313732.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDEGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31173..31985
                     /locus_tag="LZB08_000212"
     CDS             31173..31985
                     /locus_tag="LZB08_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBM7313733.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32005..33684
                     /gene="recN"
                     /locus_tag="LZB08_000213"
     CDS             32005..33684
                     /gene="recN"
                     /locus_tag="LZB08_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBM7313734.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33899..34765
                     /locus_tag="LZB08_000214"
     CDS             33899..34765
                     /locus_tag="LZB08_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313735.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKYGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKFTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34858..35349)
                     /locus_tag="LZB08_000215"
     CDS             complement(34858..35349)
                     /locus_tag="LZB08_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7313736.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35410..35850)
                     /locus_tag="LZB08_000216"
     CDS             complement(35410..35850)
                     /locus_tag="LZB08_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7313737.1"
                     /translation="MKLNVRIADINNWRDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36011..37069
                     /locus_tag="LZB08_000217"
     CDS             36011..37069
                     /locus_tag="LZB08_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBM7313738.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTIVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37211..38035
                     /gene="spo0A"
                     /locus_tag="LZB08_000218"
     CDS             37211..38035
                     /gene="spo0A"
                     /locus_tag="LZB08_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBM7313739.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38448..39569
                     /locus_tag="LZB08_000219"
     CDS             38448..39569
                     /locus_tag="LZB08_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313740.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39587..40462
                     /locus_tag="LZB08_000220"
     CDS             39587..40462
                     /locus_tag="LZB08_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBM7313741.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40486..41178
                     /locus_tag="LZB08_000221"
     CDS             40486..41178
                     /locus_tag="LZB08_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7313742.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41186..42010
                     /locus_tag="LZB08_000222"
     CDS             41186..42010
                     /locus_tag="LZB08_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBM7313743.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42015..43082
                     /locus_tag="LZB08_000223"
     CDS             42015..43082
                     /locus_tag="LZB08_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBM7313744.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43084..43620
                     /locus_tag="LZB08_000224"
     CDS             43084..43620
                     /locus_tag="LZB08_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBM7313745.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43688..44293
                     /locus_tag="LZB08_000225"
     CDS             43688..44293
                     /locus_tag="LZB08_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBM7313746.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIAVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44305..45219
                     /locus_tag="LZB08_000226"
     CDS             44305..45219
                     /locus_tag="LZB08_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7313747.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEVRPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45261..46433
                     /locus_tag="LZB08_000227"
     CDS             45261..46433
                     /locus_tag="LZB08_000227"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBM7313748.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGEVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46480..47292
                     /locus_tag="LZB08_000228"
     CDS             46480..47292
                     /locus_tag="LZB08_000228"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBM7313749.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47496..48821
                     /locus_tag="LZB08_000229"
     CDS             47496..48821
                     /locus_tag="LZB08_000229"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBM7313750.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19743 a         4921 c         9179 g        14986 t
ORIGIN      
        1 tttatttaga catttattac aaatcaaaat ttattacata ttatttataa aaaaatctaa
       61 ggaggaaata ataatgaaaa tattagtatt aaactgtggg agttcttcat taaaatatca
      121 attaatagat atgaataatg aagaagtttt atgtatagga ttggttgaaa gaataggaat
      181 agaaggttct atattaaaac atgaaaaagc aggtagagat gataaatacg ttgttgaaca
      241 accaatgaag gaccacaaag atgcaatagc attagtttta gaagctattg ctcatccaga
      301 atttggtgca gttaaagaaa tgaaagaaat agatgcagta ggacatagag ttgtacatgc
      361 tggagaaaaa tttgcaactt cagtagtaat aactccagaa gtagaagaag ctttaaaaga
      421 atgtatagat ttagctccac ttcataatcc agcaaacata atgggaatag atgcttgtaa
      481 agctatactt ccagatgtac caatggtagg tgtatttgat actgctttcc atcaaacaat
      541 gcctaaatct tcatacttat atggtttacc tcatgagcta tatactaaat atggggtaag
      601 aagatatgga ttccacggaa cttctcataa ttatgtatct caaagagcag cagagatatt
      661 agggaaagat ataaaagatt taaaaatagt aacttgtcac ttaggaaatg gtgcttctat
      721 agctgctgtt gatggtggaa aatgtgtaga tacatctatg ggattcactc cattagaagg
      781 attaataatg ggaactagat gtggagatat agacccagct atattaccat tcttaatgag
      841 aaaagaaggt ttagatgctg atggattgga taaattaatg aataaagaat ctggagtata
      901 tggaatgact ggaatttcta gtgacttcag agatatagaa gatgcagcta aaaatggaga
      961 cgaaagagct caagctacat tagaagcgta tgttaaaaaa gtacaaaaat atataggcgc
     1021 ttatgctgct gaaatgaatg gattggatgt tgtagtattt actgcaggcg ttggtgaaaa
     1081 tggaaaggct ataagagcag atattgcttc taacatggag tttttaggaa tgaaattaga
     1141 taaagaagct aatgatgtta gaggaaaaga aacagtaata tctactgctg attctaaagt
     1201 aaaaatgctt ttaataccaa ctaatgaaga attaatgata gctagagata cattaagatt
     1261 ggtaaaataa ttaaattaaa cctatgccta tcaagtaaaa atacttgaca aacaaatgct
     1321 agatttgtat aataaatttg gtgatatgtt gaggtgaact taatgaaagt tagcatagaa
     1381 aaaataaata gaaaagaaac tgacaaaata gacttgaatt tttgtgaaaa aattgatact
     1441 attagttatt gtgatgagat ttacaaatta gtatcacctg taaatttaaa agggaaagtg
     1501 tcaaagacta ataaaggttt gtatctagat atagatgtca attttacaat tgttgacaat
     1561 tgttcaagat gtcttaaaga agtagaaata ccactagagt attctataca aggtttttta
     1621 gtaaaagaag aagattatga tgaagatgag tttgaggaat ttgatccttt tatattcgat
     1681 ggtgaagaaa tcgaccttat tgatataata gaacagacat tagattttaa tgtacctcat
     1741 aaagttcttt gtagtgaaaa ctgtaaaggt ctttgtcaag tatgcggggc aaacttaaat
     1801 gaagaagagt gttcttgcag tgaaattaca aacgatgagg aatacataga tcctcgtttt
     1861 gctaaattaa aagatttatt taattaaatt ttgactaagg aggtggcgta aatggcagta
     1921 ccaaagcgta aaacgtctaa atcaaacaca aaaatgagaa gagcagctaa ctcaaagatg
     1981 gaagcaacag gatttgtaag ttgcccacaa tgtcatgagc caaaattacc acatagagtg
     2041 tgtccagact gtggatatta taaaggtaaa gaagttgtgt ctaagtaata gcttctaaaa
     2101 agcatgtaga gtatactcta catgtttttt ttgtacatat aaaaaaataa gatgatttac
     2161 tagagaaaat aagtggattt tatgcatatt aaaaaataga aaaataataa tataagtatt
     2221 gaaaattttt gtgtgttact atatactact attagtagct ggtactaatt acagctataa
     2281 aatttggagg gaacatgaaa aagaaaagta aagcccaaag acaaaaagag cttatagata
     2341 tgctaaagac agatcctttt tatactgatg aagaattatc aagtctgttt gatgtaagta
     2401 tacagaccat aagattagat agaatgagtc taaacatacc agagctaaga gaaagagtca
     2461 aatcaatagc agaaactcag agttcaaaag taaagacttt aggagtaaag gaaattacag
     2521 gagaaatcat tgatttatct gtagggcgat taggtatatc tatgttagaa gttacacagg
     2581 acatgattta ctctaagact aatactttaa aagatacgta tattttttct ctagcagatt
     2641 cattggcaat ggctataata gatgcaccta aggttattat gagagttgca aatgtaaaaa
     2701 gttttaagtt gatagaacaa caagatagac ttatagcaaa agcagaagtg tatagaaata
     2761 tagataaaaa gcattatgta aaagttgtta tcaacaataa agcccaagag caaatattta
     2821 gaggaaagtt tatctttgaa gaattagatt aggagggaaa ttatgaaaat agtaattgat
     2881 ggaatgggtg gagataatgc acctaaatct aatgtagaag gcgcagtaaa tgctataaaa
     2941 gaatatcagg ttgatttaat aatcacagga gataaagatt tacttgaaaa ggaattttca
     3001 aattatgaat ttgatagaaa taaattagag atagttcata ctactgagat tatagaaaac
     3061 gaagataaac cagtaaaggc tataagaagt aaaaaagatt catctatggt agtagcatta
     3121 aatcttgtaa aagaaggaaa ggcagatgct ataatatcag ctggaaatac aggagcctta
     3181 cttgctggtg gattatttgt tgttggtaga ataaaaggaa tcgatagacc ttgcttatgt
     3241 tcagcaatac caaatgtaaa gcgaggaatg acacttatag cagactgtgg cgcaaatgca
     3301 gattgtaaac ctaagaatct agttgaattt gcagctatga gtaatattta ttcaagaaaa
     3361 gttttaggtc ttgaaaatcc taaggtagca ttagcaaatg ttggactaga agaaggcaaa
     3421 ggcaatgatt tagtcaaaag gtcatatgaa gaaattaaaa aattagattt aaattttata
     3481 gggaatgtag aagctagaga agttataaat gcatacacag atataatcat atgtgatgga
     3541 tttacaggta atatattact taaatctgct gaaggtgtag cactttcagt tatgagtctt
     3601 ataaaggaaa cttttatggc cagtacaaaa agtaaaatag gagcattact tataaaggat
     3661 gatttaagaa aattaaaaag ttttatagat tattctgagt atgggggagc tccactttta
     3721 ggtctaaatg gaggagttat aaaagctcat ggtagttctg atgctaaagc tataaaaaat
     3781 gctataaatc aaggaataaa attttcaaag ggaaaagtcg ttgaagatat taaccaattt
     3841 ataagcaaat acaatgaaga aaataaaaat aacgaagatg aataaactcg ttttggaggt
     3901 ttaatatata atgaatacaa aggctggaat attaggagta ggaagttatc tacctgagca
     3961 atcatatgat aattttcact tcgaaaaaat aatggataca tcagatgagt ggataagtac
     4021 tagaactggt attaaggaaa gaagatttgc caaagagtct gaagcaactt ctgacttagc
     4081 atctaaagct gccttaaaag ctatagaatg tgcaaagtta aacgtagagg atatagagct
     4141 gattatatta gctacaatta ctcctgatat gtctttgcct tcaactgcat gtatagtaca
     4201 agatgcaata ggagcagtga atgctactgc atttgatata tcagcagctt gttctggatt
     4261 tgtatatgga gtaactattg caaagcaatt tgtggaaaca ggttgctata aaaatgtgct
     4321 tgtaatagga gcagaaactt gttctaagtt tttaaattat gatgatagaa caacagcagt
     4381 attgtttgga gatggagctg gagcagctgt tattggccct gtaaatgagg gtggaatact
     4441 gtctacacat atgggctctg atggtaaagg aaaagattgt ttaaaagtac ctgctggagg
     4501 ttcaagactt aaggcaagta aagagactgt agaagctaat ctacatacaa tagaaatggc
     4561 aggtagtgat gtgtttaaat ttgcagttag aaagatggca gaaacttctt taagagctct
     4621 tgaaaaagca aatctaaata caactgatat agactactta gtgccacatc aagctaatat
     4681 aagaatcata caagcttctt ctaagagatt agagcttgat atgaaaaaag tttatgtaaa
     4741 tatagacaaa tatggaaata tgtctgctgc atctatacca gtagcattag atgaagctta
     4801 tagagaaggt aaaattaaaa agggtgataa tgtagtttta gttggctttg gtggaggtct
     4861 aacttggggg gcttcagttg tcaagtggac tttatagtat aaaatatcta aaatgattta
     4921 attaacaaaa ttaaacttat acacaaaata aagttgatta ataataaaaa gtttactttt
     4981 atgatttcaa gtgaaggaga attgtatgaa taaaatttgc aaaatattaa atataaaata
     5041 cccagttatc caagggggaa tggcatgggt agctactgca tcattagcaa gtgctgtatc
     5101 taatgcagga ggacttggca taatagcagc aggaaacgca ccaaaagaag ctataaagaa
     5161 agaaattgtt gagtgtaaaa aattaacaga taaacctttt ggagtaaatg taatgcttat
     5221 gtcgccattt gttgatgata taattgattt gattatagaa gaaaaagttc aagttattac
     5281 tactggtgct ggaaatcctg caaagtatat ggatagatta aaggaagctg gaacaaaggt
     5341 tattcctgta gtacctacaa tagctttggc acaaagaatg gaaaagctag gagctacagc
     5401 agtaatagca gaaggtactg aaggtggagg acatatagga gaacttacta ctatggtctt
     5461 agttccacaa gttgctgatg ctgtaaacat acctgtaata gctgctggag gaattgtaga
     5521 tggtagagga attgcagcat catttgcatt aggtgccagt gcagttcaag taggaactag
     5581 atttatttgc agtgaagagt gttctgtcca ttcaaactat aaaaacttag tactaaaagc
     5641 aaaagataga gatgcaattg taacaggaag aagtactggt catccagtaa gaacattaaa
     5701 aaataaacta tcaaaagaat ttttaaagat ggaacaaaat ggagctactc ctgaagaact
     5761 ggataaaaaa ggtacaggag ctttaagatt tgcaacagta gatggagaca tagaaaaagg
     5821 ttcatttatg gcaggtcaaa gtgctgctat ggtaaaagaa ataacacctt gtaaggaaat
     5881 tatagaggct atggtaaatc aagcaagaga gattatgcca gcaatagaac tgtaaatcag
     5941 gaggagcaag atgggaaaag ttgcactagt attccctggt caaggagctc aatatgtagg
     6001 tatggccaag gatttatatg aaaataatac tgttgctaaa agtgtaatag atgaggctag
     6061 tgatgcctta aatatggatt taaaaaacct tatgtttaat ggaaatgaag aagaactttc
     6121 taaaactgaa aacactcaac ctgctatggt tacacatagt gtagcagtat taaaagcggt
     6181 acaagctcaa atagatttaa aatataatgc atgcttagga ttatccttag gagagtatag
     6241 tgcgttggtt gcagcagatg caattgactt taaagatgct gtatgtttag ttaaaaaaag
     6301 aggtaagttt atgcaggaaa cagttccagc aggtgttgga gctatggcag caattttagg
     6361 tttagataga aatgtacttg aaaatgtggt taaagatata caaggaggta ttgtagaagt
     6421 agcaaattac aattctcctg gacaaatagt tatatctggt gaaaatgaaa agatagaaga
     6481 agcaatggta aaatgcaaag aagcaggagc aaaaagggca gttaaattaa atgtaagtgg
     6541 accttttcac tcatcaatgt taaaagaagc tggtgtaaaa ttggccagtg aattagaaaa
     6601 agttaacata actaaaccta aagttgatgt ggtggcaaat gtaaatgcag attactataa
     6661 aaacgaaggt aaagacctgc ttataaaaca agtaagttca tctgttcttt gggaagattc
     6721 tatagaaaga cttttaaatg atggatatga tacttttata gaaatgggac ctggaaaaac
     6781 tttaaaagca tttattaaga agatagcttc caataaaaaa gcaaatgtca atatttataa
     6841 tatagatggt atagattctc ttaatgaatt tgtacaaaat tatagaaatg gagagatata
     6901 atgataaatc ttacaggaca agtagcagtt gttactggtg gctctagggg tataggaaaa
     6961 gaaatagcaa aaaaactagc atcttttgga gctgatgtag taatcaatta tacttctaaa
     7021 gaagatgaag cactaaaaac taaaaatgaa atagaaagta tgggggtaaa gtgtacctct
     7081 ataaaatgtg atgtatctaa atttgatgaa gtgaatcaaa tgatagattc tgttgtaagc
     7141 gaatttggaa aaattgatat attggttaat aatgcaggca taactaaaga tggtctgctt
     7201 atgagaatga aagaagaaga ttttgataga gttatagata taaacttaaa aggtgtcttt
     7261 aattgtacaa aagcagttac taaacctatg atgaaaaaga agtatggaag aataataaat
     7321 atgacttcag tagttggaat tatgggtaat gcagggcaaa ctaattattg tgcatcaaaa
     7381 gcaggtgtaa ttggatttac aaaagcttct gcaagagagt tagcatcaag aaacataaat
     7441 ataaatgcag tagcacctgg atttatagaa acagatatga ctaaagtact aagtgatgat
     7501 gtaaaagaat caacactagc aaacatacca aagaaatctt atggtaaacc agaagatgta
     7561 gccaatgccg tagcattttt agttagtgac atgtcaagtt atataacagg acaagtaata
     7621 aatgtagatg gtggaatggt aatgcaataa taatttaaaa agtagagaaa tctattaaaa
     7681 taaaaaattt taggaggtac acaatgttta ataaagttgt agaaataata atagagcaat
     7741 taggagtaga agataaagaa ataactatgg agacatcatt aatgaaggat ttagaagcag
     7801 attcattaga tgcagtagaa ataataatgg cacttgaaga tgaatttggt atagaaatac
     7861 cagatacaga agcagaaaac ttcaaatcta taggagatat agttaagtat atagaagcta
     7921 ataaatagtc gagctttaaa aaagtggagg tatccgatga ataaaagagt agtaataaca
     7981 ggacttggat gtgttacgcc gttgggaaca ggaaaagaag agttctggag taatataaaa
     8041 tctggagtat ctggtataga taaaataact aattttgatg ctagcacata tcaaactcag
     8101 atagcaggag aagttaagaa ttttcatcca gaagaatata taagtaaaaa ggaattgaag
     8161 agattagata aatttgctca atttgcaata gtatctgcta aattagcagt tgaagatgca
     8221 aatcttgatt tagataaagt tgatagagaa cgttttggtg ttataatagg ttctggaata
     8281 ggtggagttg aagctataga aactcaacat aaaattcttt tggaaaaagg aaataaaaga
     8341 gtaagttcac tttttgtacc tatgatgata ggaaatatgg cagctggtca agtttcaata
     8401 tttttaggag caaaagggcc taacactaat gtatgtacag cttgtgcatc aggaactcat
     8461 tcaataggtg atgcttttaa ggtaattcaa agaggtgatg cagacattat ggtagcaggg
     8521 ggttctgaag ctgctgtaac aggtcttgct tttgctggat tctgtaacat gaaggctatg
     8581 tcaactagaa atgatgaccc taaaactgca tcaagaccat ttgataaaga cagagatggt
     8641 tttgtaatgg gagaaggagc aggtattgtt atcttagaag acttagaaca tgctttagca
     8701 agaggtgcta aaatatatgc agaagtagtt ggatatggtt taactgctga tgcatatcat
     8761 atgacaactc cagctgaaaa tggagaggga gcagctaggt ctatgaatat ggctttgaaa
     8821 gatggaaatg ttccattaga agaagtagat tatataaatg ctcatggaac ttcaacttat
     8881 tacaatgatt tatatgaaac tatggctata aaaactgtat ttggagaaaa ggcatatgac
     8941 ttatgtgtgt catcaacaaa atctatgact ggtcacttat taggagcatc aggtgctata
     9001 gaggctgttg tttgtgctat gagtatagaa gatagttttg tgcctccaac tataaacata
     9061 caagaagttg gtgaagattt ggatttagat tatgttccaa atcaaggtaa agaaaaaaat
     9121 attagatatg cactatcaaa ttctttagga tttggtgggc ataatgctac aatagtacta
     9181 aaaaaatatg tataaataaa tattaaatat aaaactaaaa aacaagcact tgttttaact
     9241 gtataaattt atattagctt aaatttaaag tctcctaaat tattttagga gactttttta
     9301 ttaagattta agtctaggat atgttgtaaa tcattttgat atattgtatt attaattatt
     9361 aagaggttat ttatatatgc aagttttcat tctacaatat acaaaaggag gactaaaatt
     9421 ttgaatttta gactaccaaa gaaaaggtta cccaaaaaga taatttttat atgtataata
     9481 cttattagta ttattttgtt tataaagtat gaccaaaatg gattgaaaga aagagaaatt
     9541 ataaataagg gacaatatat gtctgaaagc cttgtggcag ataaggagta tgatgaagca
     9601 aaaaaagtgg ttaagacttc tttcgaatct attccagaaa aaaaatacaa taaagtatat
     9661 aatgaagttt gggatatgtt aaagactatt tcatatgttc cagatggaac gaagatagtc
     9721 attgattctt tagaaaagat gagagaatca gatgtgaaat tatctgatga atgtagattt
     9781 aatatagaaa aaagattagc atcagtgtat ataatggata ataattattc taaggcagta
     9841 gatttaactg taaagtctat aaagttggct gagaaattgg ggaataatta tgaaaaagca
     9901 agactagatg ttgatttggc gagcatattt ttaaagatag gtggctatga aacaggagag
     9961 aaattaataa aggattcttt taaaatagat attgatgaag gtgaaaaaaa tggaatggtt
    10021 agattatatg ctattataaa tctagcagaa atatatgtag aagagggaaa atatgataaa
    10081 gctattgaaa taagtagcag gataaaagat tataaaaagt ttgtaaatac ggataattac
    10141 aatgattttg atataatggc ctctataatg cagtcaaatg cgtatgtggg caaaaataat
    10201 atacaaaagg caaagacttt tttagaaaaa gctgattatc ttataaaatc agatagaaca
    10261 gtttatatat tagataaaga tatgtattat tatatggcaa tgggaaactt agaatataaa
    10321 agcgaaaatt ataattctgc aatagataat tataaaaaaa gtttagagat atctaccaaa
    10381 agaaagttaa ctcaggagaa aataaaaaat ttaaataaga tattaaatat atatgaaaaa
    10441 caaggaaata tagaatcttt aaatgagtat caaaagatgt taataaatga atataaagag
    10501 aacaaaagta taagagattc agagactagt ttctatataa ttgataaagt atcaaacgaa
    10561 agtaagctat ttgaaaaaac taaaaaagaa ataaaatatt ataaaatatt atttacagta
    10621 attttaattg ctatattagg gtctactttt ttatacaacc gattgaagta ttttaaagca
    10681 caaaatttac atgatggtct tacaaatgtt tataatagaa aaagttttga tattatgtat
    10741 gaaaagtaca gagagaaaga tgataatttt gcacttgtga tgatagatat tgataatttc
    10801 aagctcataa atgataatta tggacataaa tttggtgaca ctgttataaa gggaataaca
    10861 aataccattt gtgttatgtt agaacaagat gataaagtct ttaggtacgg aggagaagaa
    10921 ttttctgtat taataagaaa taaatctggt gaggaagtag cagatataat tgacagtata
    10981 agggttgcaa ttgcaaataa aaaatggaat gatgatgtga ctgtgactat aagtgcaggt
    11041 gtagctcata tttcagatag caaagatgta ctagaagaag ctgataaaaa tttatataaa
    11101 gcaaaacaac ttggtagaaa taaagtagta tataagtaat tatatactat ataaataaga
    11161 ggtataaata tgaaacaatc aatagctata gtaactgata aaataactaa actatcatct
    11221 ttcttagaag aaaatatacg tttggtttta ggtaattata ttgatataaa ccactattat
    11281 ctggaagttt taaaagagaa tgataaaata caaggtgatt ttgtactagt aatgaatgat
    11341 gatagattaa acagtatgag aaagcattta gtacaagata gtaagattat agtgataagg
    11401 agaacactta aagaaaatga aatatatgga ttattttcaa taccaaatgg aactgaagta
    11461 ttggttgtaa atgatacaaa agaaactacc ttagaaacga taagcttatt ttacaaaata
    11521 ggagtaaaaa atttaaagtt tactccatat attgaaggaa aaatttataa aaatatagac
    11581 atagctataa cgcctggagt aaagggaaaa gtaccaagtt atataaataa agtaatcgac
    11641 cttggaaata gatatattga tatatctacc tttatagaaa taataaatgg tcttagaata
    11701 gatgttaaag aaatacgtaa aaatttaatg gcatattctg agagtttaat aagtttagat
    11761 aatggagtta aagataatta taggcaactg tttttaaaaa tagaagaaca agatgttata
    11821 ttgaatctat caaaagatgg aataatattt acttcagcag atggtataat aaacacattt
    11881 aataaagaag ctttaaaaat tttaggtata aatgaaaatc cacaaggcaa aaacatagaa
    11941 gatatcattt ctgaagattt aaatatttta cttgaagaga aagaaataat tgatgaagtg
    12001 gtatttacaa ataaaaaata tataaatgta aataaaaaaa gtattttttc tatggggaat
    12061 aaagctggga tatattataa cttacaagaa ataacgtata taaaaaaatt agaacaaaat
    12121 cttactaata aattaagaga acaaggtcag atagcaagat atacatttaa agatataaaa
    12181 actaaaaata aaaatatggt aaagtgtgta gaattggcta aaaaaatatc taaatcagac
    12241 ctttctgtat taataatagg agaaagtggt actggtaaag aattattatc acaatcaatt
    12301 cataatgcct ccaatagaag taatcaacca tttatagcag ttaattgtgc agcagttcca
    12361 gatagtctat tagaaagtca actgtttgga tatgaaaaag gctcttttac aggagcttta
    12421 agagaaggaa aaaagggtct atttgaactt gctaataatg gaactatatt tctggatgaa
    12481 attggagata tgcctccatt gctacagaca aagttactaa gggtattaca agaaaagcag
    12541 gttatgccta taggttccca taatgttata aacatagatg ttagggtcat agcagcaaca
    12601 aacaaaaact tactaaagat gattagagaa ggaaaattta gagaggacct gtattataga
    12661 ctaaatgtat tgccaataga tgtaccatca ttgaggaata gaaaagaaga tataataaca
    12721 cttatgaatt tctttttgaa aaacaatata agactttcgc cagaagttat aactatttta
    12781 gaaaaatata attggccagg aaatataaga gagttacaaa atgttgcttc ttatgtaagt
    12841 ctaatgtgtg atagtatggt ttttagagat gacttaccac catatataag ggtagaatgt
    12901 gctaacaatt atgaagatgg atttggttat acaagaaaag aatttgagga aatactgagg
    12961 attctatatg aaagaagaga gtcatcctct ggaattggaa gagggtttat aatcgaaaag
    13021 cttatacagg aaggtattga gatttctgaa agcaaagtta gaaaggtgct atcttattta
    13081 agtgaatgtg gatacatatc atctaaatct ggtagaggag gaagtcaaat aacctcagag
    13141 gggataaaac tttataataa tataaattaa gctaagtatt tgatattgaa atcacatatt
    13201 ttgaatttag atatttaaat aataataaac ttttatattt atagagatta atttatatta
    13261 tgtcagaaag aatattgtaa tttataatta aatattaata ggtttttaaa ataggattag
    13321 ataggtaata ggttaagtta gaataaccca ttacctattt ttatttgtaa gaaaagtgta
    13381 catagaatta gaagtaaaaa atacaactaa taaaactatt gaaaatacta gataaataat
    13441 aaataaaaat aattatagat tttggcatac atattgctaa ttatagagat aagacgacaa
    13501 aataattggg aggtttaatg atgaaataca aaacaacagg gacttgtgct acagaaattg
    13561 aatttgaagt aaaagaaaat aaagttacaa atgtaaactt tataggtgga tgtgatggaa
    13621 atttaaaagg gctaaaagta ttagttgaag gcatgaatat agaagatgta atacaaaagt
    13681 taaaaagaat agagtgtaaa acaaagccaa catcttgccc agaccaactg tctctagcat
    13741 tggaaaatta tatgaatata aagtaataca taaaacaatc gattagttag gagtttaatt
    13801 atgaaaccaa taatagggat tttaggaaat ttaataataa tggaaaatgg tatgtttcca
    13861 ggtcttgaaa ggtcatatgt aaataatgat tatataaatg cagtgctaaa aggtggcggt
    13921 agtccagtaa taattcctgt aaatacagat aaagaagtta taaaaaaaca gattgaaatg
    13981 gttgatggag tcttaatatc tgggggatgg gatatcaatc ctcaactgta tggagaagaa
    14041 acaagagaag aaacgacatt tatatatcca gaagtggatg aatttgattt aattgcaata
    14101 agtatagcat tagaacttaa aaagcctata ttaggtgtat gtaggggact acaaatttta
    14161 aatgtaagtt taggaggaac tttatatcaa gataataatc ttatagaagg aagttatata
    14221 aaacatacac aatcttctaa aagacatgta gctactcata aagttgatgt taaagaagga
    14281 agtattttag agggaatttt aggaaaacaa ttacttacaa atagttatca tcatcaaagt
    14341 gtcaatcagc ttggaaaagg attaaaagcc atagcatact caaaagatgg tataatagaa
    14401 gctattgaga aagaggatga aaattttgta gtaggagtac agtggcatcc agaaatgatg
    14461 gtagattact gtgataaaat ggaaaaacta tttaaatatt ttataaatat ttgttcaaat
    14521 ttataatata ccttgggggg attgaacatg aacgaaaaga acaaaatggg attaataagt
    14581 attatacttt taggtattaa tgcagtagta ggagctggag tttttctatt accaggagat
    14641 gcaatgaaat catttggtgt agcaagtata tttgtatata tatttgatat gttgttagtt
    14701 ttatctatgg cattctgttt tgctgaggtt gcaggtaaat tcaataaaaa tggtgctgcc
    14761 tatgtatata caaaagaagc ttttggagat ttttgtggtt ttgaagtagg tcttatgaaa
    14821 tgggtaatag gatgtatttc ttggggagct ttaatagtag gttttcctac atcattatca
    14881 gcagtatggg ctccagcagg tgaaccacat atacaaaaaa ttataattgt agtcatgata
    14941 gttgggctta caattataaa cttattaggt gtatctctat caaaaattgt acaaaatgtt
    15001 ataactgtag gtaaactaat tccattaata ctgtttatag gaataggtat attttttata
    15061 aaaggagtta attttacttc ttcaacaatg gtacctccag gagctggagc aacagagttt
    15121 ggagcagcag cacttttaat gttttattca tttacaggat ttgaatctat agcagttgca
    15181 gcagaagata tggagaatcc acaaaaaaat ataccaatag caattataag tgtaatagta
    15241 attgcatcaa taatatatat attaaatcaa gttgtatgtg ttggtatact aggagattcg
    15301 ttatctagta catctacacc agtggcagat gctgcaagaa tatgctttgg aaatatggga
    15361 gctggattgg ttacctttgg aaccttggtt tctgttggtg gaatttgtat gtgtggtgct
    15421 tttgtaaatc caagaagttg tgtagcttta gctgatgata aaatgttacc gagaatattt
    15481 gcaagaaaag ataaaaaagg tacaccatat gtagcgataa tagctactat gttaatcact
    15541 ataccaatag ctctatctgg aagttttgct gaattagcag caataagtgc agttgctaga
    15601 ttcatacaat acataccaac atctttatcc gtattagtgt ttagaaagaa aagaccagaa
    15661 ttagtaggta cttttaaaac tccttttgga gcagtgattc ctctaattgc tgtatgtgtt
    15721 ggaacatggt tattattcca agcatctatg catcaattaa ttatgggatt aggagctttg
    15781 gcaattggag taccattata ctttataatg aagtcttata atagaaaagt ctatggtgaa
    15841 aatttaaaga aaatagttta attatgttat aacagtttat tgagttaaag ttaggtattt
    15901 gcagaaaatt aaatacaaat aagtatctat aaggctatat taaaatatta ttaatttttt
    15961 attttaatat agccttaaat tgttatcaat aagtgaaaaa tattgtaaca atagaagaac
    16021 aatagtatta tgtttataga caaaaaactt attaggaggt aaatttatgc caacaataac
    16081 attaaaaaat ggtgtagacg tacttattag agaaggagta agggaagatg ctcaaagcat
    16141 tatagatttt tataatgaag tgggtggaga aactcatttt ctgtcatttg gaaaggatga
    16201 atataaaata tctttggagg aacaagaaaa tgctatagaa tcagcaaaag catccgataa
    16261 ttctgttaaa ctgattgcat ttatagatgg tgaaatagtt gggattgcaa cgattgattc
    16321 taatcaaaaa gcaaaaggaa agcatgttgg agtattagga attgttgtaa aagaaaaata
    16381 ttggggaatt ggattgggta aaaggcttat gttagacctt atagaatggt gtaaatcaaa
    16441 tggaataacc aagaagataa ctcttgtcac taatgaagaa aattataatg ctataggatt
    16501 atacaaaaaa gttggatttg aagtagaaag catactaaag aaagaatgtt attataatgg
    16561 tgtttatact gatttaatag gaatgtcact tttattggga atataattag tgtacatttt
    16621 atctcgaaaa aagtatatta agtatggtat aattaaaaaa gaatataact taatataatt
    16681 ttggaggacg aattatgaaa aatctgtgta aaatatcaga tgattattta aacagcaagc
    16741 ttaagtattt aaagttatta tcaaaacaat atccaagtat atcaaaagca agtacagaaa
    16801 taataaattt agaggctata ttaaatcttc caaagggaac agagcatttt ataacagatg
    16861 tacatggtga atatgaacca tttgtacatg tattaaaaaa tggttctggt gtaataaaga
    16921 gaaaaataga agaattgttt tcaaatacaa taagagacag cgaaaaaaaa atgttagcaa
    16981 ctcttgtata ttatcctgaa caaaagttag acctaataat taagcaagag gaaaatatag
    17041 atgattttta tagaataaat atttatagac ttatagaact ttgtaaatat gcttctagca
    17101 aatatactag gtctaaagtt agaaaactgt taccagaaaa ttttaaatac ataatagaag
    17161 aacttttgca tgagcatgtt aaaagtgaac ataaagaaga gtactataaa agtatagttg
    17221 aaacaatagt tgacatagga atagctaagg agtttataat tgctatttct acagttatac
    17281 aaaaattggt tgtagataga cttcatgtaa taggagatat ttatgataga ggtccaagac
    17341 cagatataat tgtcgataag cttattgaac atcattgtgt agatattcaa tggggaaatc
    17401 atgatatatt gtggatgggt gcagcctcag gtgaaaaaac ttgtatagca aatgcattga
    17461 ggatatcagc tagatatgcg aatttagaca ttgtagaaga catatatgga ataaatctat
    17521 tgcctttagc cacttttgct atagaaatgt ataaagatga cccttgtaaa gaatttattc
    17581 caaaggttaa tgaccaaagt gtaacaacaa cagaaaagtc tttaatggca aaaatgcaca
    17641 aagcaataag tataattcaa tttaagcttg aaggtgaggt tataagacga agacctgaat
    17701 ttgaaatgga acatagactc ttgcttaata tgataaacta tgatgaaggt acgattaatt
    17761 taaaaggtaa aacatataaa ttaaaagata cttatttacc tacaatagac aaaaaagacc
    17821 catataaatt aactatggaa gaaagaaatg ttattgataa attagtatct tcatttagag
    17881 gtagtgaaaa attacaaaaa catgtttcat ttttgttttc taaaggaagt atatatttaa
    17941 aggctaactc aaatttactg attcatggat gtgtaccgtt aaatgaagat ggaagcttta
    18001 tgtctatgaa tataatggga aaagagtata aaggtaaggc tcttatggat aagatggagt
    18061 ctctagcaag agaaggattt ttctttaaag ataaggcaga agaaaaactg tatggcatgg
    18121 atattatgtg gtatttatgg actgggaaat gttcatcatt atttggtaaa gatgatatga
    18181 ctacatttga aagatacttt atagctgaaa aagagactca taaagaaaat aagaacccat
    18241 attttaaact tagagaaaat gagatggctt gtaaaagatt atttgaagaa tttgatttag
    18301 aacttgatga atctcatata ataaatggtc atgttcctgt ggagagtaaa aatggagaga
    18361 gcccaataaa agctaatggt aaaattctcg ttatagatgg tggtttttct agggcttacc
    18421 aaaaaactac tggtatagct ggatatactt tgatatacaa ttctagaact ttgcaattag
    18481 tatcgcatga accatttaat tctgctgaag aagccattgc aaatgaaagt gatatcttat
    18541 cgacaactgt tgtagtagag cataaggcaa agagaaaaat ggtgagagat actgatgaag
    18601 gagtaaagat acaagaggaa atagaagatt taaaactact tttaatggct tataagaaag
    18661 ggctaataaa agaaatgtag atgttataga ggcacttcat gattgaagtg tctttttgtt
    18721 aatacttatt aaatatatta taaagattaa taatagtagt ttatattata cttactaagt
    18781 gaatccaaga taaatgggtt cacttttttt atttataaac attattaaaa aaatatagat
    18841 gttttggtta tattccttgt cctttataaa tagaattata taaacaaagg aggaggacaa
    18901 gagatgaata aactttctga tgaaataata aactctcttt ctacaactat aagagaaaag
    18961 gtagaaaaag tctcaaacaa taatctgaac atagaagaaa ttaggttacg ctcacaaaaa
    19021 ccattgatac taaatgcaaa ctctaaagat tatttttaca atcaaaaaac tatgacactt
    19081 gatttaaatc aacaaaactc atatgtagta acaagagaag atgtagagca aacttttcaa
    19141 ataatatgta aatattcaat acattcattt atggatgaca taaaaaaagg attcataact
    19201 ttgagaggag gtcatagagt agggttagtt ggaaaagcaa tagtagaaga tggacaagtc
    19261 aaaaatataa agcatatatc atctttaaat ataagggtat caagggaaat tattggatgc
    19321 tcggataaga ttttaagtca tatcataaag ggaaaaaatc agattaataa tactttgata
    19381 atatcaccac ctcaatgtgg aaaaacaact ttaataagag atatagtaag aaatttaagt
    19441 aatggtaatg aagattatgg atttaaaggt ttaaaagtgg ctttagtaga tgagcgtaat
    19501 gagatagcag gagcttatct aggtgttcca caaatggatg tgggaataag gactgatata
    19561 atagaaactt gcccaaaaga cttgggtatt acaatgcttt taaggtctat gtcaccaaat
    19621 gttattgtga cagatgaaat tggaagtgaa aaagaaataa aggcattata cacagcatta
    19681 aatggaggta taggattaat aactactgtg catggagatt caatagagga tatacaaaat
    19741 agaaaagaat taaatagact tctggataaa gagttattta aaaaggttat aattctttct
    19801 gcaaaaagag gagcaggaac aattgaaaaa atttatgatt tagaagaaaa gagatggtat
    19861 tttgcaaatt aaaataatta ttatagcttt cttgatagga agtagttatt tgataggtga
    19921 acaaatatac aaaacttata ctcgtagaca taaacaatta aatgatttaa taagagtttt
    19981 ggaaattttg aggatggatt tatcttttgg actttataca ttagaagaaa tctttaatag
    20041 gataggtgga aataaagagt tttgtttttg gaaatttttc tatcaaatat cagaaggact
    20101 tcacaatgaa caaagtaaaa ctttggaaat aataatatca gaaaatattg atgtcttaag
    20161 taaagaaact tatctaggca ataaagaaat tgaggaactg aaaaatctaa tacttacttt
    20221 agggaaaagc gatatagagt cacaacaaag aatgatagat ttatctatag aaaatttgaa
    20281 aaaacaaacc tatgagacaa aggaagatat aaacaaaaaa ggcgtattgt ataaaaaatt
    20341 agtgacattc ataggtatag gtatatgtat tatattgatt taatatgggg aggtttatta
    20401 tggaaatatc attgatatta aaggttgcag gtgtcggtat tctaatatca gtattgaata
    20461 tgatattgga aaaaacagat agaaaagatt gggcaggtct tactacatta gcaggtgtaa
    20521 taatcgtatt gggtatggta ataacagaaa taagtgactt gtttaatact gtaagaacaa
    20581 tgtttcaact ttactaaaga ggagggatta atttggaaat aatgcaatta ataggaattg
    20641 caatcatttc aactacactc tgtttagtaa ttaaaaaaga tagaccagag atagcaaatt
    20701 tcatagctat aataactgga gtaataatac tattgtcagt tatgtttaag ctaaatttta
    20761 tagtagatag tatacaagat ttggctaaca aggctaacat cccgactatg tatatatcat
    20821 taataataaa attaattggg atagcatact taatggaatt tgcgatacaa ctttgtaaag
    20881 attgtgggga aggaaacatt gcttcaaaac tagaatttgg aggtaaaata atagttatgt
    20941 caatgtcttt ccctatactt ttgtccattg tagaaatggt ggtgaatata attccataga
    21001 tgtgtatctt ggaatgaata taagtatatt agagagcttt cgttgtattg aggtttatag
    21061 agtgaagaga ggcatggttg cttatgaaaa aaagattttt accaatgatt ataggttttt
    21121 tatttacttt cttttttgta aatatctttg ctattatgat atttgctaat gaagtaccaa
    21181 gtagtgaaga taaagaatat ggtgaaacaa aaaatagcat agataagtat atagatggtc
    21241 aattagacaa gctagatata aatgagattc aagactatat aaataaagaa atagttatta
    21301 atgatgttaa tttaaaatca tttgtaaaag atttaattag tggagaaaaa aatatcttag
    21361 atttattcaa taaagatgga ttgaagatat tgatgtttga tgaatttaaa gcaagtttaa
    21421 aggtagtagc agtaattttg gtactggctc tgttatcatc tattttaaag agtctggaaa
    21481 actctttttc atcaggtgca gtaagtcaaa tagcaacata tataatattt atcacaatgg
    21541 tatcacttac acttattggt tttaaagatg tattacagat ttgctatgat gcaatagacc
    21601 atactgtagg tcttatgcaa gttataatgc caatattaat aacattttta cttttaattg
    21661 gattcccaat aacgtcgact actttgaatc ctatattcat aggaggagta acatttataa
    21721 atgtattttt taaaaatttt ctatttgtat ctattactgt tgcatttgga attttaataa
    21781 taaacaattt atcaaaaaat ataaggctaa agagattttt ctcctttgta aaacaaataa
    21841 attatgtttc tattggagct atgtttactg tttacttagg tcttgtatcc atacaaggat
    21901 tgtatgtaac cagttttgat aaattcagtg taaagactgc taaatttgct ataggtaatt
    21961 ttattcctat tgtaggtgga tttgtatctg actctgtgga tattttatta tcttcatctc
    22021 agcttataaa aaatatattt ggaggaatag ggcttatctt acttgttgga atttgcttac
    22081 ttccagtaat aaaaatttta tcagtaatag tagtgtacaa attagcagca ataattgtag
    22141 agcctgttgg agaggatgga atatctaatt ttttaaatga agtggcaaat ttaatgataa
    22201 taatgctagc atcagtaatt gctataacag taatgttctt tgtaactgta gctatcttga
    22261 cctcgataag tgtcgtaagt caagggtaat ttatatatca gtgaaaaata attacattta
    22321 atctaatgtt tttagagagg aggatgctat gttagagggc ataaaggcat ggatagttag
    22381 tgttttgata ggagcattta tagtaaatat agtagatatg attttaccaa gttctaagat
    22441 aaaaccttat gtcaacctag ttttaaactt tatgtttgta tttattgtaa taactccagt
    22501 agtaggattt ttttcaaaag atatgagttt agaagataga atattaaaat ctatgggaaa
    22561 ctacaacaag cagtatgtag atagtacaaa tgctttggca aaagagactg gaaataatag
    22621 tctatccaag ggatatgagg atgggttaaa agaagtactt aaattaaaac ttgatgaata
    22681 tggttatgat ttagaagata tagagcttaa tggggcaaat ataaataata taaaaataaa
    22741 agaaaagaat aacagtgcaa aaagtaataa cagttccaat gaagaaaata aaaataatag
    22801 tactaaaaga gaggaagaaa acataaactc taatgacaaa gaaaattcta aacaagtctt
    22861 caaaaaaggt acagagtatg ggttgaacct aaatgaagaa aaattaaaaa acgacttaat
    22921 aaaggtgctg gatgtttcta tagaagatat acaaattgat aaataggagt tgggagaatg
    22981 ttgaaaaact taaatgacaa agataagaga aaggtatatt cactaataac tatagcagga
    23041 atatgtgtgg tttcattagt attattatct tgttttccta gtagcaaaag cgataaacaa
    23101 gttggaaaaa cagagactaa taaaaatgct gaaaagcaag tcactaaaga acaagaaaaa
    23161 gatgatttag agtcaaaatt aacagcaata ctatctaaaa ttgatggtgc aggagacgtt
    23221 gatgtaatgg ttacatttga atcaagtgaa gaaatacaac ctgcttttaa ttcaaataat
    23281 acaacagaaa caacagagga aaaagatgca caaggtggag aaagaacagt tacgacttct
    23341 agtgaaaata aaacaatgat aacttcaaat tcaagtgacc ctgtagttat aaaaactaca
    23401 gagccaaaga taaagggagt gatagtggta gcaagtggag caagtgaccc aagtgttaaa
    23461 gagacacttt atagtgcagt acaaacatca ttacaagtag ctggtcatca agtagaaata
    23521 tattctaaat aaatacatat aaccgggggg ataaatcaat gaagtttaat tataagggaa
    23581 gaggatttgt aataataact ttaactgcaa tgttagtggt agtaggaaca gtgaattatc
    23641 aattaagtaa gaaatcttta ttagaaacat caaaagagtt taaggcatat gaagaagcac
    23701 agttgcaaaa aaatactgat gagagtgata gtagtaagac agaggattct agtaatatag
    23761 ataaacaaga tggtaaggag agtgctgata ttgatatagt cgatagcaaa gctagtaaag
    23821 taaaagaaaa ggcaactgag acaagtaagg aaataaaagc tcaattatca tcagagaaaa
    23881 atatgaaaaa ggcatcttat attttagata tgaaaatgaa tagagaaaaa caaagaaatg
    23941 agttagttca agatttaaat gaaatgataa ataatccatc tacaacagaa gaatctagaa
    24001 aagaagcttc taatatgaaa ttaaatatag ttaaaattca agaaaaagaa tttcaaatag
    24061 aaaacctttt gagtacaaaa ggatatgaag aagcgttagt ttatataagc gataacaaag
    24121 ttaatgtagt tgtaaatgaa gctaaattag aaaagaaaga tgcagcaaaa atatttgatt
    24181 tagttgcaga acaagccaat gtgaagtatg aaaatattaa acttacaaat aataatagta
    24241 ataagtaaag tataaacctc agaattaact gaggttattt tcttgcaaag tagacttgta
    24301 tttggtatta ttatacttat aaaaatagtt ccttaaaatc aggaggtaag ttttaatatg
    24361 gaagataata aatttggaca agtaaaaata tctaatgatg taatagctac aatagctggt
    24421 ttagcagcat tagaagttga aggcatagaa actacagcaa cattaacaga taagttgtta
    24481 aaaaataatg gagtaaaaat acaaatcgaa gaagaggatg ttaatttaga tgtaatggtt
    24541 acgataaagt atggaatgtc aataccagat acagctttta aagttcaaga aaatgtaaaa
    24601 aatactgttg agacaatgac aggactgaag gtttctcaag taaatataca cattcaagga
    24661 atcagcttta agaaggataa agttgataaa gaagaagcaa aagcaactaa gaaaaattaa
    24721 tattagccct ctgaaaagtc agagggtttt ttgctggtaa ttgattttat atggataatt
    24781 aaacttaaag gagaattttg attgatgaaa aaagataggg cacaaaagag tacaacaaga
    24841 gaatatataa tgaaacttat ataccaaata aatattaata aagaagattt tgaaacttta
    24901 gaagataaag tagataattt tttaaaagat aattcagaac atattataaa tagatataaa
    24961 gaacttgcac ttcaatactc aaaaaataca aatttaaagt tagaagatac tgaaattgaa
    25021 gatgttatag acaaaaaata tataaataca gtatgcaaag cattgaaaga aaatcatgac
    25081 aaaatagatg agttaataaa taagcatgct aaaaactgga cagtagatag aatgccaaag
    25141 gtagatgttt ctatacttag attgtctgtg tgtgaaatac tttatttaga cactcctaat
    25201 aaagtatcaa taaatgaagc agtagaactt gcaaaaatat attgtgatga taagtctcct
    25261 aaatttataa atggtatatt gggaagtgtt gttgatgaaa ttgggaaata atataataat
    25321 tggaattgat actagctgct atactacttc tatagcagct atctctttag ataagaaagt
    25381 tatattcaat gaaaaaataa tgttagaagt aagggataat tcaaaaggtt taaggcaaag
    25441 tgaagctgta tttcaacata tcaataattt agggattctt agtgatagga taaaatcttt
    25501 taaagataag tttaatgtag aaggagtttg ctcatcaaaa aaaccgagac ctgttgaaaa
    25561 ctcatatatg cctgtattca atgtaggtca taattttggg aaattattgt ctagtatata
    25621 tggttgtagg ttttatgaga ctactcatca agagaatcat attgaagcaa gtttattaaa
    25681 tagtaaatta aaaaataata ataaatttat ttctgttcat atgtctggtg gtactacaga
    25741 gatactttta acaagcaaac aagatagtca tcacaatgta tgtgatacta atttaggtaa
    25801 aattgccaaa ataagtatta aaaaagatga taaaagtaag ttatacaata attttggata
    25861 taatatagat ataattggag gaagtaaaga tattagcttt ggtcaactta tagatagagt
    25921 aggaattaag cttggatata aatttccttc aggaaagtat ttggatgaaa atgctttaaa
    25981 ttgtaatctt aaaatagaaa gtggtctgaa aacttctgta agagatgggt atatgaattt
    26041 atcaggatta gaaaatcagg taaataagat tataaatgat aatggagata atactaatca
    26101 aaaagaatat atatctaaat tggtattaga ttcagtagtt agaaatatgt ttaaatcttt
    26161 agtgtattta tgtgaaactt ataatgtaaa tgaagtagta tttgctggtg gagtttctgc
    26221 aagtaagtat attcttagag aattaagcat gaagttaaga aaaaagcata tagaagctta
    26281 ttttacagaa ccacaatatt caactgataa tgctgtcggg tgtgctataa taggattgaa
    26341 taacttttta ggagaaagag tatgaaacta agagctttag atataagtga agcaaactca
    26401 tatattaaaa gaattttaat aaacgaccca atactttcta atcttaaagt taaaggagaa
    26461 atttctaatt ttaaggttca tagtagtggg aatgtatatt tatcacttaa agatgaaaca
    26521 tcaaagttaa actgtgtaat ttttaaaagt aattttaatc gcaatttaaa attagataat
    26581 ggtgtaaaaa ttatagctaa tggatatata tcagtctatg aaagagatgg tgcgtatcaa
    26641 ttatacatta atgaaattga aatagaggga ataggaaatt tacacataga gtttaataga
    26701 ttaaaggaaa aacttaataa agaaggatta tttgacccta aatacaagat acctatacct
    26761 aaaatgccaa actcaattgg cgttataaca tctccaacag gtgctgttat aagagatata
    26821 ataaatgtaa taaaaagaag atatccgaaa gttaatataa agctttatcc agtaacggtt
    26881 caaggagata aatcagctga agaaatctgt gaagcaatta gatttttcaa tcatatgaag
    26941 aatgttgata cacttatagt tggtagaggt gggggttcga tagaagaact ttggtctttt
    27001 aatgatgaaa tggtagcaag agaggttttt aattctcaaa ttccgataat atcagcagtt
    27061 gggcatgaaa cggattttac tatttgtgac tttgtgtctg atatgagagc cccaacgcca
    27121 tcagcagcag ctgagattgc tactccatca ttggatgata taaactataa acttggaaac
    27181 ataaaaagta ggatgagtaa gtctcttaca aatcaaatag agctagacca atataggtta
    27241 gaaacagtat ttaataagat aaataactat ttagattcgt acacaataaa agataaagtt
    27301 atacaattag acaaaatata tgataaaata atttttggaa tagaaaataa tttaaaatta
    27361 gaagatgaaa aattagtaaa aattggagca ttactccata atttaagtcc attagctact
    27421 atggatagag gttatagtat aactcaaaaa aatgggaaag taataaatag tataaaagga
    27481 ttaaaaataa aggatagtat agatatagta ttaaaagatg gaaatttaga atgtatgata
    27541 gataaaattg aaaataaaga gggatagtat gaatttaacc tacgaagaag cttataaaag
    27601 attagaaagt attttaagtg aattagaatc taaaaatgct agcttggatg aatctttaag
    27661 cctatatgaa gaaggtataa gcctttataa acattgtaat aagcttttag atgatgctaa
    27721 attgaaaata agtaaattta atcaattggg aatagaagaa gactttaaaa tagaggagga
    27781 ataaaattgg aatttaaaca atgtctaaaa gaaaaagcaa gttttgtaga aaaagtactt
    27841 aaagaatata tgcctaaaga agaagggtat caaaaaactg ttattgaagc tatgaactat
    27901 agcttaagtg ctggaggtaa aagattaaga ccaatactta cattggaggc ttgtaaaata
    27961 gttggaggta atgaagatga agctattcct tttgctattg caattgaaat gatacataca
    28021 tattcactaa tacatgatga tttgccagct cttgacaatg atgatttaag aagaggaaga
    28081 ccaacaaatc ataaagtata tggagaagct atggggattt tagcaggaga tgctttgtta
    28141 aactatgctt ttgaagtaat gcttgcaggc tcaatcaata aagaaaatcc agaaaagtat
    28201 ctaaaagcta taaatgaaat tgccaaaggt gctggtatat atggaatgat aggtggtcaa
    28261 gtagtagatg tagaaagcga aaataaacaa attgaaaaag aaaaattaga ttatatacat
    28321 atgaataaaa ctgctgcaat gatggtaggc tgtatgagag caggagctac tataggtgga
    28381 gcaaattctg aacaaatgga agaaataact aaatatgcaa aaaatatcgg attatcattc
    28441 cagatagtag atgatatact agacatagta ggagatgaag ctaagttagg taaaaaggtt
    28501 ggaagtgata tagaaaatca taagtcaaca tacccatctc ttttaggttt agataaatct
    28561 aaagaaattg cacataattt gattgatgaa gcaaagaaaa gtatagaaaa attatctgat
    28621 gatgtagatt ttttaaaggg attggctgaa tatataatag atagagaata ctaaaatata
    28681 cagctaaaat cgaggaggga aaaatggatt ttttttcgga gatttttaac aatggggctt
    28741 tgggaataag tctgattgct tgtttcttag cacaatttat aaaaatattt actggtaagg
    28801 aaaaaagaat tgagttatca agaatactta tttcaggagg tatgcctagt tcccatagtt
    28861 cttttgttac aagtttagct acagtggtag gtattgaaaa agggtttaat tctactgatt
    28921 ttgctataat taccgtgctt gccttaatta taatgtatga tgcagcaggt gttagacggg
    28981 ctgttggtaa acaagctact atattaaatc aaatggtggc agatatacag catggaaagc
    29041 atatagagca gaagaaatta aaagaattaa taggtcatac accactagaa gtttggtttg
    29101 gagctttact tggaatagta acagctttaa tattgatgta aattgaaaat ttacagaata
    29161 aaggtgagga taatatgtat aaatatttag ataaagtaaa ttctccaaag gatataaaaa
    29221 atatgagtat agaagaaatg gatttacttg caaaggatat aagaaagttc ttagtaaaat
    29281 ctgtttcaaa gacaggagga cacttagctt ctaatctagg agtggttgag ttaacattag
    29341 cacttcataa agtatttgat agtcctaaag ataagattgt atgggatgta gggcatcaat
    29401 cttatgttca taaaatagtt actggaagaa aagattgttt tgtatctttg agacagttta
    29461 atggactaag tggttttcca aaggagaatg aaagtcctca tgatattttt gatacaggac
    29521 atagcagtac atctatttct atagctacag gtattgcctg tgctagggat ataaaaaaag
    29581 aaaactatag tgttatatct gtgataggag atggttctat aacaggaggt atggcacttg
    29641 aagctttaaa tcaattagga tatatagata ccaatatgat agtaatcctt aatgataatg
    29701 agatgtccat agataaaaat gttggaggaa tgtctaaata tctgtcaagt attataagaa
    29761 actcaacagt tgagaaaatg acagatgaag ttgataaaat tttaaatgtt acacaaacag
    29821 gtgaaatttt atctaagact gcacatagat ttaaagataa actaatgtat agcttttctc
    29881 ctcaggactg ttcatttttt gattcattag gtataagata ttatggacct atagatggcc
    29941 ataatactaa agaactaata gatatattga gaaaagctaa acacaaaaaa gggcctgttc
    30001 ttttacatgt aattacgaaa aaaggaaaag ggtacagatt tgcagaagaa caacctgata
    30061 aatatcatgg agtatcaaag tttgatataa agacaggtgt tacatctgca aaagttaagt
    30121 ctatgtcaat tagtgttgga gaaaaacttg ttgatatggc taatagtaat gaaaatatag
    30181 tagcaataac agctgcaatg ccatctggta cagggttaaa cctatttgaa agtgcatatc
    30241 caaaaagata ttatgatgtt ggtatagctg aacaacatgc aacaggattt gcagcaggtc
    30301 ttgcaaaaaa tggtatgaaa ccttactttg ctgtgtactc atcatttttg caaagggcat
    30361 atgaccaagt tattcatgat gtatgcataa caaaaaagcc agttactttt cttatagata
    30421 gagctggatt agttggaaat gatggagaaa ctcatcatgg tatgtttgat ttaagttact
    30481 taaattctat tccaaatatt gtagtaatgg ctccaaaaga cacaagagag atggagctta
    30541 tgatggattt gtcattaaaa ttagattgtc cactagcaat tagataccca agaggaagta
    30601 gttattacct agataaggga gagtatggag agattgtact gggtaaatat gaggtattag
    30661 atgagggtca agatacagtc atactttgta ttggaagtat ggtaaaacat gctttagagg
    30721 ctaaagaaat attatcaaga gaaggaataa atccgacaat agttaatgct agattcttaa
    30781 agccaattga tgaaggtatg ttaaaagcat tattaaaaaa tcataaaaat gtagttacta
    30841 tagaggataa tattgtaact ggaggatttg gaagtagaat aaataagttt attatagata
    30901 atgaatataa cgtaaatata cttaatatag caataccaga agaatttgta aaacatggca
    30961 atatagatga attatatgac tttgtaggat tatcgccaaa aagtattgct gataagataa
    31021 ggaaattagt tattgagtaa aaatctaatg tagatattgt aaattaaaat ttatataaaa
    31081 tatagaattt atcaattaga ataaatatca agtgtgttac ttcaagctac tttagttatc
    31141 attaaagtag tttttaacag aaaggtaatt gtatgaaaaa aagaatagat ttattattag
    31201 tagaacaagg gtactttgaa agtagagaaa gagccaaaaa agcaataatg gctggattgg
    31261 tttttgttga taatcaaagg tgtgataagg ctggtacaga agtaaaagaa gattgtagta
    31321 tagaagtaaa aggaaatcca ataccatatg tcagtagagg tggtttaaaa cttgaaaaag
    31381 caatgaaaaa ctttgatttg actattgatg gaaaggtatg tatggatata ggggcatcca
    31441 caggtggttt tacagattgt atgctcaaaa atggagcaat aaaagtattt tctatagatg
    31501 ttggttatgg tcaacttgca tggaaattga gacaagatga tagggttgtg tgtatggaaa
    31561 gaaccaatat aagaaatgtg acaatagaag atacaaagca gtttgctgat tttgcgtcaa
    31621 ttgatgtgtc ttttatatcg ttaaagttag tacttccaaa ggcaaaggag ttagtacgtc
    31681 atgatgggga agttgtcgct ttaattaaac ctcaatttga agcaggaaga gaaaaagtag
    31741 gtaaaaaagg agtagttaga gaaaaatcta cacatataga agttataaaa atgatttctg
    31801 atttctcagt cgaaaatgga tttgagattt tagggttaga tttttctcct attaaaggtc
    31861 ctgaaggtaa tatagaatat ctaattcatt tgagaaatgg aaatgaagga tatgaatttg
    31921 atggtgagac atacaataac aaaatagtag aagttgttga agcttctcat aatttagaca
    31981 aataggttaa aagggggctt agatttgatc ctcgaattat atatgaaaaa ttgtgcctta
    32041 gttgaagaac taaggttgaa tattgataaa aacctaaata tacttacagg tgaaactggt
    32101 tcaggaaaat ctataataat agatgcactt ggactttgtt taggtgataa atatgatagg
    32161 tcttttttaa gaaaaggaac agataaggga cttgttgagg ctgtattttt ttcagacaat
    32221 agatatctta aaaaaatatt agaagaaaat gatataagta tggaagatga taatttattg
    32281 gtaataacta ggttgatata ctcagatggt aagagtactg ctagagtaaa tggaaggact
    32341 gttaaagtgt ctcttcttaa agaaattgca tctacactta tagatataca tggtcaacat
    32401 caaaatcaag ctttgtttaa taaagatact catttaaaat ttctagacct ttttggagaa
    32461 aatgaattgg aagagtttaa aattgcttat aaaaatgtgt accataagta tagtgaagta
    32521 aaaaaagctt taaattgctt gacagaaaat aaagatgaga tgcaaattca acgagaaata
    32581 gatttattaa gatttcaaat aaatgaaatt gaagctgcaa atctaaataa gaatgaatat
    32641 gaagatttat taaaacaaag agaagtttat agaaatagtg aaaagatata taataatcta
    32701 aattcgagtt atagcaagtt acataatggt gagtataatg ttattgatct aataggtctt
    32761 gcatctaagg aacttaatga catatctaaa tatgacagtg ttttaagtga atatagtgat
    32821 actgtagaaa gaatcatgta tgagttacaa gacatatctg gtgagataag aaattacaaa
    32881 gataatatag actttgaacc ttatgagcta gagcagattg agcttagaat cgacgagatt
    32941 aataacttaa gaagaaaata tggagacagt atagatgata tatttgagta ttatggaaaa
    33001 attaaagata gattagatga aatattaaac agagatgaaa gagttgaaca gttaagaagc
    33061 aaactgatga atatagaaga agatttaaaa ataaaagcca gtaaattgac taaagcaaga
    33121 atagaagtag caactacttt agaaaaaata cttcttgatg aattaaaaag cttaaatatg
    33181 aaaaatgtaa tgttcaaggt taattttgaa aaaggtccat ttactttaga tggagtagat
    33241 gatattgaat ttatgatatc ttttaactta ggagaagata ttaagcctat atataaagtt
    33301 gcatcaggtg gtgagatgtc cagatttatg ttagcgttta aaaccatatt ggctgatata
    33361 gatgatattg atacattagt gtttgacgag atagatactg gaataagtgg gatagcagca
    33421 cagatagttg gtgagaaatt gagtgatata gcaaagaaaa aacagataat atgtataact
    33481 catttacctc aaattgctgc aaatgcagac actcattatt gtatagagaa agatacttca
    33541 aataatagga cgtttaccaa tgttagtaag ttaaatgaat ctcaacgaaa aaatgaaatt
    33601 gccagactta tagcaggtaa taatataaca gaaaaaacta ttgaacatgc aagtgaaatc
    33661 attgaattgg ctaaaaaatg ttagtgaaaa acaagcattt taataggtgt aaagaatttt
    33721 tatagaaatg ctttacacct attttttata atgaaattat tttaaaatat ctagtgccta
    33781 tattgaagaa aagtaggttt gaaaatggaa taaaatttgt ataaatttgt aactttgaaa
    33841 tttctaaatg cttaagtata atattattta ttaaggtagt agtgagggga ggcataaaat
    33901 gaataaaaaa gatactatgc acttaatttt tataattgta atttttattt tattatggta
    33961 tattgtggta gcagtaagtg agattaataa aaatattgaa agttgtagta acaaattaga
    34021 ttatttgtca caagagctta tgagtactaa gtcggatatt agtaatacta taaatgaaga
    34081 aatgtcaaaa agttatataa caaaaagtat tgacttaaaa gtaaaaaaaa tcgagaaaaa
    34141 agaatgtatt attgatgtag atgttcaatt aagtaaactt ggaaaaaatg gaaaagtatt
    34201 ttttatgtat aaagaagata gtgataaatg gaatgaaact gagatgacaa aatatggaga
    34261 attatcatat gcatgtgaaa taaagataaa tacaggaagt gaatatgact ataaagtagt
    34321 tacaagtgga gctatatctg aaagttctga tgtgcaaaaa tttactaaat ctgattatat
    34381 gcctgaacaa cccatattta attctgggat aagagataat agagaatact ttattgaaat
    34441 agtagaaaat agtaatcttt tcaatcatga gagtgaaaag tcaaaaaata aagcttacga
    34501 taatattaga ttagaaaaag tagatattat tgtaaatgaa ggaaaaaaag acactatata
    34561 caaagccaaa ttagctgaag gcttagatga tggcaaaact aataattcaa atagaaatca
    34621 agtgaattat gaagtaagtt ttcctaaaag ggatattgat gacataattt atattaaagc
    34681 taaacttaca tacaataatg gagtagaata tacaaaggat ataactgaag atataactat
    34741 gggattacaa gatttagaaa aataaataca tatttaaata aatattcaaa tagatttgtt
    34801 caataaaata gagtataaat aaaactgtag tagttttact tatactctat taaaaaatta
    34861 taattttaat tccatatatc tagccccttc aattggatta taagtataag gttctatatc
    34921 ataaaaacca aacttttcat ataatccttg tgcacttttc atgcttggaa gagtatctaa
    34981 tctcatgtgt gtatatccaa attttttagc ttcttcaatg atttcttcca atagtatttt
    35041 tcctattttt aatcctctaa attgatttct tacatataat ctttttaatt cacatatttt
    35101 accttcaagt ttttttaaag caacacaacc tgccaaattt tcatcaacaa atgccaatat
    35161 caatgagcct gaaggttttt tatattttcc tggaagtgtt tttaattcat tattaaagtc
    35221 ttgaaaacat aagtctatat ttagagaatt tgaatattca gtaaatagaa gttttacgtt
    35281 ttcaaggttt tctaaatctt tttcttcaac aaattgtaaa gttatatttt gagccatttt
    35341 atccgccatt atatacacat gtttatataa tgacttcacc tccttgatat atgtatattt
    35401 ttcttaatat caattagatt ttaataccat tactttttca ccaccatagt caagctctcc
    35461 attaaactca aatccaaaat ctttataaag cttaatagcc attttattgt cttcgaatat
    35521 actaagatat agttcatcac agttatattc atttttaaga tgatttacaa gaaaatctaa
    35581 tgatgctttt ccatatcctt taccttgata ttttgaatca atcataaatc tatcaagcca
    35641 aactctatca tcttctaaac gaccatacat agcaaatcct attaaagaat tttcatcata
    35701 tattccaaca ggtacccaag ctgttataaa tttagattca gcaatagaaa gtgcattgct
    35761 ttctatataa tctaactgac ttttatctac agacaaacta actaaatcac gccaattatt
    35821 tatgtctgca attctcacat ttaatttcaa gatattaccg cccttcaaac tttttgttac
    35881 aatctaatac tatcatatta tgtattaaaa atcacttatt aagattttaa ataaactttt
    35941 taaatgaata aattctcagg aaaaaggaat tataaataca taataataca gcataggagt
    36001 agatggatat atgaacaaaa taaataaaaa tttcaaaaat atttggataa tattaggagc
    36061 tgtatcgatt tttaccatag ttataatttt taataaaaat tgtaatgtta ataaagagac
    36121 tcaaatagaa gcttttacaa ttaagaatca tgataaaaaa tttgtatatc ctatgggaaa
    36181 tataattgga gtaaaagcaa atacagatgg tgtattggtc ttaggatatg aggaagagga
    36241 tgtagactat attggaggca tacaaatcgg tgataatata gtaaaaatag ataataaaag
    36301 gataaaaaat agtcaagatg tatcagagat tttgaataag attaaaaaaa gtaaagttga
    36361 agtgactttt gagagaaaaa ataagtataa aacagaagta atagagacta aaaaagaaaa
    36421 tggcaagtat aaacttggat tatgggtaag agataaaata tctggaattg gaactatgac
    36481 tttttacgac cctagtatgg aaaagttcaa agcaatcggt catgccataa aagattcgga
    36541 tactaatgaa ctattaaaga taaagcaagg atatatatat aagccagaac aattaaaaat
    36601 agtaaaagct agtaatgaaa aagtaggtaa aataaaagga gattttaatg acagcaattt
    36661 aatgggtaat ttctcaaata attcagagtt aggtataagt ggtaatatta cagaaaacca
    36721 taataaagag tttaatgtag caaataaaga aaagcaatta atagaagttg gaagaccgca
    36781 agatgtaaaa ataggagatg cagtaattct ttttgaggat aaaaacaaaa atataacaag
    36841 ctatgatata aaaatagaaa gtatagtata tgataaagga aattatagag atatggtaat
    36901 aaaagtagta gatgacaagt tattggaata cacaggaggt atcgtacagg ggatgagtgg
    36961 agctccaata atacaaaata ataaaattat tggtgcaata actcatgttt ttagagataa
    37021 tccgaaaaaa ggatatggta tttttataga tgaaatgata aaattgtagg tgaggcatta
    37081 aaaaatttta ttattttatc aattatctag gaggaatata attttggagt gtcgaatatg
    37141 ctttagagta gataattagg aagcaattgt gtaaaaagtt tagttttctg taataagaag
    37201 atgtttttta atggggggat ttttagtgga aaaaatcaaa atagttttag cagatgacaa
    37261 taaggatttt tgtcaggtat taaaagagta tttgtctaat gaagatgata tcgatatatt
    37321 aggcatagct aaggatggaa ttgaagcatt agacttagta aaaaagacac aaccggactt
    37381 attaatacta gatgtaataa tgccacatct agatggatta ggtgtaattg aaaaattgaa
    37441 tactatggat atacctaaaa tgccaaaaat aatagtacta tcagcagtag gtcaagataa
    37501 gataactcaa agcgcaataa atctaggagc agattactac atagtaaagc catttgattt
    37561 tgttgtgttt ataaacagaa ttagagagct agtttctaat agagttacac aagttgagcc
    37621 aaaacctaga ccagttcaag agactcaaat gactagaagt gattttgtta agaatgtagg
    37681 aaatatagag acagaaatca caaatataat acatgaaata ggagtaccag ctcatataaa
    37741 aggatattta tatttaagag aagcaataaa aatggttatt gataatgttg agcttttagg
    37801 tgcagtaaca aaagagttat atccaagtat agctaaaaaa ttcaatacaa caccaagtag
    37861 agttgaaaga gcaataagac atgcaataga ggttgcatgg agtagaggaa aagttgacac
    37921 aataaatcaa ttatttggat atacggtaca caatactaaa ggaaaaccaa ctaattcaga
    37981 atttatagca atgattgctg ataaattaag actagaacat agtatggtta aataaacaag
    38041 acataaaaag taaggctttt taattaaggc attggctata aatgcgtatt acaagcagcg
    38101 aaacggtata accactaggg ttataccgtt tcgctatttt aataaatata aaaattttct
    38161 ttattatttg catactatat caatataata attttattat actatggata tagtatgtgt
    38221 ctttacaagt tgtaaactga cagtggttta ttttttaata taaatattga ctttgatgca
    38281 ggtaaacttt gtatttttaa gcgtattatg gaatatgtta aataaaaaaa tgatgaaata
    38341 tagtattgta aatgccaaag atgcaaaaca aacttaaaac atttatttta ttgttaagta
    38401 atgctataat ataatgtgat tttaataatg atagtggagg tttaaatatg agagtcgagg
    38461 cccctataaa agtagatcga aaaaccaaaa aacttgctaa aagagttgaa agtggggaaa
    38521 tagcagttat aaatcatata gacatagatg aagttgctgc aaactcttta gtagaagcta
    38581 aaataaaact tgtcataaat gcggctcctt ctataagtgg taggtatccc aataaaggtc
    38641 cagggatatt aactgaaaat aatatactaa taattgataa tgttggagaa gagttatttg
    38701 aagaattaaa agaaggagaa actatagaag ttgtagatgg aaagatatat agaaaaggaa
    38761 aatttttagg tgcaggagaa gttttagaca agtatgaggt ttcacaccaa ataaaggctg
    38821 cctatgagaa tttagctgta gaattggata gatttataga taatactata gattatgcta
    38881 aaaaagaaaa aggatttata cttggagaag tagaaattcc aaaagtgaaa actaattatg
    38941 ctaataaaca tgtattgata gttgtaagag gtcaggacta caaagaagat ttaagtacta
    39001 tgctttctta tatagaagag gtaaagccag tattggttgg tgttgatgga ggagcagacg
    39061 ctttaattga gtttggatat actccagatg tgattgtagg tgatatggat agtgtaagtg
    39121 atgaagcttt aaaaaaggct agtgaaatag tagtacatgc ttatacagat ggaagagcac
    39181 caggtcttaa gagggttgag gaattaggct tagatgcagt tgtatttcct gcgccaggaa
    39241 ctagtgaaga tatagctatg cttatcgctt atgagtataa ggctgagtta atagtagcag
    39301 ttggtacaca ttcaaatatg attgattttc ttgaaaaagg aagaaaaggt atggcaagta
    39361 cttttttagt tagactaaaa ataggttcaa agcttataga tgctaaaggc gttaatttgt
    39421 tatacagaag taaattaaaa ataaagtaca tatgggcatt aatagcaaca gcattatttc
    39481 ctgtgttggt agttgcatca ttgtcaccag gagttcaaca gttcatacaa ctaatgcaat
    39541 taaagtttag agtattatta cagatgtaaa ttttaggagg ataaatatgc atattaatat
    39601 gaaatattat atagtcacta taggggctat atttatagct ttgggtatag gcatgttagt
    39661 aggatttaat ttaaataata atcaacaatt aagtgaacaa caagcaaaca taataaatga
    39721 tttggatgat aagtttgata tcttaaaaga aaaaaatgat aaacttgaca gtgacttagc
    39781 aagtgtaaat agagattatg aagaggcagt taattttata aataaaaatg tagataagat
    39841 tttagctggt tcgctgaatg gtaagagtat aggaataata tctgctaatg aaaatgacga
    39901 ttatacaaaa aatatagaag acataataaa taaatctaat ggaagcattg catttaatat
    39961 aatattaaaa gaaaacataa caaatccaga aaagttaaag gaaatgtcaa ctaaactagg
    40021 tacagatgta aaaaatgcaa atgatgcagt taattatata attgacactc ttaaaaagga
    40081 agatgcaagt gacattttaa catatcttca agaactagat gtaataaagt ttaattttat
    40141 tggagataca tatctaaaat atgattccgt tgtaatagct ggtggaaatg atgcaaaaga
    40201 cagtacaaaa cagtttgaaa aaatagaaaa atttgtagtt tcaaaactta aatcagagaa
    40261 taaatattta gtagaagttc aaaatacagg agttaaaact tcttatgtag aactatattc
    40321 taaaaacaaa gttgcaacta tagacaatat tgatgaaggt ataggaagta tatctctagc
    40381 gattcttcta caacaaggta atatagttgg taattttggt agattggata ctgcaacttc
    40441 attgttgcca tctattaaat agaaataatt ttaaggagag aaaaaatgga ttcttatata
    40501 agtataataa taccagctta taatgaagaa agtagaataa aatatacatt agaaagcata
    40561 ttggacatta agcaaataaa tgaaataata gttgtggatg atggttcaat tgacaatact
    40621 gctaaggttg tatctgagct agaaaatgaa aagattaaat tttttaagtt agataaaaat
    40681 agaggtaaag gatatgcttt aaattatggt atcaaattag caatgaaaaa tgcagatatt
    40741 ataggttttc tggatggaga tttaggaagt tctgctaaag aagttgaaaa attaatagta
    40801 cctatattaa atggtgaggc ggatgttaca atagccaagt ttcctcctgc aaagagaaaa
    40861 ggaggtttgg gatttgtaaa aggtttagct aaaaattctg tgtttgagat gactggtgtt
    40921 gagttagatg caactttgtc aggtcaaaga atatttaaaa gagaagtttt agaaaagttt
    40981 gatgaaatac cttttggata tggagtagaa gttggaatga ccattgatat attaaagtat
    41041 ggatttaaaa ttaaagaagt attagtaaat atgactcata atgaaacagg aagaaattta
    41101 aaaggtttta ttcatagagg gaaacagtat tatcacataa aaaaggtttt aggacaaaaa
    41161 aagaaagaat ggaggtaata tcttagtgaa agattatata ctttatacca ttttattatt
    41221 aactggatta tttggaacct atgctgtaat tccactattt aaaaatttat tgataaatgg
    41281 aaatgtactt agaccaaact ataaaaaaga tatgatacca gtaagtatgg gaattgtatt
    41341 tttgcctatg attgtaatca atggaataat acttgctttc tttactacag aatttaagga
    41401 tttagcgtat atatttatgt ttatgtttgg tatgatatcc atgttttttg cagggatact
    41461 agatgatgtc attggaaata gagatgtaag tggtttaaag gggcatttta aaagtttatt
    41521 taaaggaagt cttacaacag gaggttttaa agctttattt ggtggatttg tagggcttat
    41581 agtttctgtt gctatatcaa aagacataat tgatataata gtaaatacac taataatagc
    41641 tctatcaact aatttaatga atcttttgga tttaagacca ggaagggcaa taaaagcata
    41701 tttattcata atggtaatta tatttttaac attgacagga tttgttcaag ttcttccatt
    41761 acttatagta ccaaatgtac ttgcatactt taattatgat ttaaaagcaa gggctatgat
    41821 gggagatact ggttcaaacg tccttggaat atctattgga atgctcattt cttttgggta
    41881 tccatttaac attagattag gatggttaat atttgtagtt attatgcata tattcactga
    41941 gaaatactca ttaactaaga taatagaaaa aaataaacta ttaaatttta tagataaatt
    42001 gggaaggtag tattatgata agcaaaagag tggggattgt tgaatcaata gtaagtcaaa
    42061 ctgaaactct agatgatata agagtaaata taaatggaga aatacaaaag gcatataatt
    42121 atccaaaaat atctggaact ataaatatag gtgacgaagt ggttttaaat acaacagcag
    42181 tagaactgag cttaggtaca ggtggatacc attttgtaat aactaattta aataatatag
    42241 aatctacttt aactgaaggt ggacatataa tgaaacttag atatacgcct cttcaaataa
    42301 aagtggattc tgttgaggaa caagaaagta tttatcatga taaatttgcg aattttaaag
    42361 gattagaagg tctttctgtt gtagtaggga ctcttcatag tatgttaact ccatttgcag
    42421 cttcatttaa aagaaataat ccaaataaaa aattagtgta tataatgact gacggagcat
    42481 ctcttccaat ttatttaagt aaaaatgtgg atactttaaa agaaaaaaaa ttaatagata
    42541 gtacaataac tataggaaat gcgtttggtg gagattatga atgtataaat atatatactg
    42601 cattaattac agcaaaagaa atactaaaag cggatgcagt atttgtcagt atgggacctg
    42661 gaattgctgg gacaggaaca aaatatggat ttacagggat tgaacaaggc tctattcttg
    42721 atgccgtaaa aaaacttgaa ggaagagcaa ttgcaattcc aagaattagt tttgctgaca
    42781 aacgagaaag acatcaaggt atttcacatc atagtatgac tgtatttaaa gaaattgtaa
    42841 atgtaaatgt agacatacca ataactatat atgatgatga aaaattgaac tttataaaag
    42901 agcaaatcaa tttaaatggt ttagacaaaa agcataatat aatttatata gataataata
    42961 aaacgaaaga ggatttagac tattttaatc taaaagtaaa gagtatggga agaaattatg
    43021 aacaagatga ggcatttttc aaagctgcaa gcacagcagc atattactta atggaggttt
    43081 aagatggtat tagaagaaaa aactattagt agtgataggg tttatacagg taaagtaatt
    43141 actttaaagg ttgatactgt tgaaattcca gggcaaggat atcaaaaaag ggagttagta
    43201 gaagttggtg gagcagtagg aatagttgca atcacagatg ataataaagt tgtgttagtt
    43261 aagcaattta gaaaacctat agaaaagcca atctttgaaa ttccagctgg taaactagaa
    43321 aaaaatgaaa gtccaaaaga atgtgctgaa agagaattaa aagaagaaac tgggtatagt
    43381 gcaaaaaata ttaagctaat acataaattt ttcacttcag caggtttttc taatgagata
    43441 atgtttgtat atttagcaac aggtcttact ccaggagaga ataatttaga tgcagatgag
    43501 tttttagatg tctatgaaat tgagcttgaa gaagcatata acatggtatt aaaaaatgac
    43561 gttgaagatg caaaaacatc tattggatta ttattagtaa aagatatgtt taaaaattaa
    43621 tatttataaa atgaataacc ctttgtccta aagcatatta ttttataaag gacaaggggg
    43681 aattaaattg agaagaacat atagaaaaaa tgattttaga gaattaaata gacaaataat
    43741 tattatagga ttgttgttta tgatgtctat tgctgttggg tcttacataa ataaaatatt
    43801 gccaggttct agtaacaata tattaaacaa tataaatcct gctgtagagt attataatct
    43861 aaatataagc ataaaagata cagtgataca aaatttaaaa tcagatgcaa tctttatggg
    43921 ctcaatagct ctattaagtc tatttgtagt aacaattcca gcagtgttgg tagcttttgt
    43981 attaaaaggg atgtcaattg gttatacaat aaatagttgt atattggcat taaaatttaa
    44041 aagtataaaa atgattttaa taatattatt taaaaatttg attattattc catgtgcaat
    44101 catattagca ttaattagtt taagttattt taaagaaatg gtttatgaat ttaaaaagaa
    44161 aaatagaaaa aatatgcaat ttttaattaa gaggtatata ctaaatatta taattataat
    44221 agcactatca ttggggttgc aattaatatt aaatactgca agtattggta ttattaagtt
    44281 cctagctaaa taaaggagtt tataatggat attatagagg gatatataga ttacataaaa
    44341 gataaaaaaa aattatcaga gaatacagtg gcatcatact ttatggatat aaaaaaatat
    44401 atggaatata taaatcaaaa aagcattaaa ctagtagata tagtagaaaa tgatattata
    44461 agttacttaa taaagttgga aaaagataat gtatcaatag ctacaatagc gaggatgata
    44521 tcctctataa aatcttttca tgattattta tttttaaatc atatatgtac aaataatcct
    44581 gcaaaggata taaaaaaacc taaaataaaa aaagaaaata taaatatact tacagaagaa
    44641 gaaattgaaa gactattaaa ttttccaaag ttaacaacgc ctaaattgat tagggacaag
    44701 gctatttttg aagtattata tggcactggt ataaaagtat ctgaattagt agagatgaat
    44761 atagaggata ttgacctaga tattgattat atatattgta attcatgttg taaaaatcaa
    44821 agagtgatac cactttgtga tattacaaaa ctttacttag aaaagtatct aaaagaagta
    44881 agacctaaaa tggctgtaga aggtgaaaaa tcattattcg taagttcatt aggtcaaagg
    44941 tttacacgac aaggtctttg gaaagtcata aaaaaatatt ctaatctagc caatatagat
    45001 aaaaatataa atcctacaat gcttagacat tcatttgcta ttcatttgtt aaatgaaggt
    45061 gctaacattg ctgttgtaag taaaatttta ggaaatgtta atttatctag tttgcaagta
    45121 tatttaaatc atatagataa aaacgtgaga agagaaataa aggagaaaca tcctagaaat
    45181 gatgttgatg tagaattaca aaaagcagaa gctaaataga ctatatagca aattttataa
    45241 tttagaagga gcatatttat atgagcagag taatttggat tgtaattgat agtgttggga
    45301 taggagcatt acctgatgct gaaaaatttg gtgatagtaa ggatgtaagt acccttggaa
    45361 atatatttaa ggaatatcca gatattcaga ttcctaatat gagaaattta ggtataggaa
    45421 acatagatgg tatagatttt tttgaaagta taaaagaacc aataggttgt tttggaaagt
    45481 gtaaggaaat gtctcaggga aaagatacta ctacaggtca ttgggaaatg actggtataa
    45541 tagttgacaa accatttaag acatttgaac atggattctc taaggagata atagaagagt
    45601 ttgagaaaaa aactggaaga aaagtagtag gaaataaacc agcttcagga actgtaataa
    45661 tagatgagta tggagagcat caaataaaaa ctggagaggt aatagtgtat acatcagctg
    45721 atagtgtatt tcagattgct gctaatgaag aagtaattcc cctagaagaa ctctataata
    45781 tgtgcaaaat cgctagggaa ataatgatgg gggataatgc agtagctaga gtaattgcta
    45841 gaccattcat aggtaagaaa aaaggtgagt ttgtgaggac ttcaaataga agagattatt
    45901 cactagaccc atttgaacca acagttcttg ataatataaa agaatcagga cttgatgttt
    45961 tagcagttgg aaaaatagaa gatattttta atggaaaagg aattacagat gctatccaca
    46021 ctaaaagcaa tatggatgga gtggatgaaa ctttaaatta tatgaaacaa gataataaag
    46081 gtctaatata ttcaaatctc gttgattttg attctaaata tggtcatagg agagacccag
    46141 aaggatataa gaaagctctt gaagagtttg atagtagact tcctgaaata atggctaata
    46201 tgagagaaga tgatatttta ataatcaatg cagaccatgg aaatgaccca acatataaag
    46261 gtacagacca tacaagagaa tatatacctg ttatgattta tggcaataag attaaaaaag
    46321 gttttaattt aggagtaaaa gatacttttg ctgatattgg agcaactgtt gcagatatac
    46381 taaatgtaaa gttgcctaaa catgggtcaa gctttaaaga agatttattt taaactataa
    46441 aatgataaaa acttagctta ataggatgga gagattggaa tgtttgaaaa gatagcacaa
    46501 agtagtaaat ttataaattc taaaagtaat ataaaaccta agattggttt gatattaggt
    46561 tctggactag gtgatttagc aaatgatata gaagaccctg tcacaattaa atatagtgaa
    46621 attccaaact tccctgtatc aacagtagca ggacatgctg gacaacttgt aattggaaaa
    46681 cttgaaggaa aagaagttat tgctatgcaa ggtagatttc attactatga aggatattct
    46741 cagaaagaag ctacttttcc agtaagggtt atgaaagaat taggagtcga gattcttata
    46801 gtaactaacg ctgctggagg cgtaaataaa gaatttaaag caggagattt gatgcttata
    46861 agagaccata taaactttag tggaagtaat ccattagtag gaaaaaatga tgacagattt
    46921 ggtgctagat ttccagatat gtcagatgca tattcttcta aatatgtaga tgtagttaaa
    46981 aactgcgcta aagaatgtaa tatagatgtc aaagaaggcg tttatatgtt ttttagtggt
    47041 ccaaactatg aaacaccagc agaagtgaga atggcacaaa ttttgggagc tgatgctgta
    47101 ggtatgtcta ctgttccaga agtcatagtg gcttctcact ctaatatagg agttattggt
    47161 atatcttgta taacaaatat ggctgctggc atcttaaatc aaccactaag tcatgaggaa
    47221 gtaatagaaa ctactcaaaa agttaaatca gaatttatga atttagttaa aacaacggtt
    47281 aaaaatttat aagttaaagc ttattataga ttttttgagg aagtttataa taaaatagtt
    47341 aatgtttaat ttgaaaagaa ttaataaaaa gctgtcttga tatagaaagt tatttctaat
    47401 aaaggacagt ttttttataa tactacaaca tttgaaagtg ttttaaagtt taatatttat
    47461 ttaaattatt ataggttaga atggagattt caattatgag aatgtatgat attataaaaa
    47521 aaaagagaga taacctagag cttagtaaaa aagagataaa ctactttata gaaaactatt
    47581 caaagggaga tatacctgac tatcaagctt ctgcactatt aatgtcgatt tatttaaata
    47641 agatgaataa acaggaaact gtacatctta cagaagcaat gatgaactct ggagacatga
    47701 taaacctatc tgagatagat ggaataaagg tagataagca tagtactggt ggtgttggag
    47761 ataaaactac catagctctt atacctttgg ttgcttcatg tggagcacca gttgcaaaga
    47821 tgtcaggtag aggtcttggt catactggtg gtactattga taaattggag tctataccag
    47881 gtttttcgac agaaatggag ataagtaaat ttattgattc tgtaaataag tctaaaatag
    47941 cagtttgtgg tcaaagtgca aaggtagcaa ttgcagataa gaaaatatat gctcttagag
    48001 atgttacagc aactgttgat aatatatcat tgatatcatc aagtgtaatg tctaaaaagt
    48061 tagcttcagg agctgatgca attgtgctag atgtaaaaac tggtgatgga gcatttatga
    48121 agacattaga agagtctttt gagttagcta aatctatggt tgatataggt actggtatga
    48181 atagagatac aatagggatt gtaacagata tggatgaacc tttaggtttt gcagtgggaa
    48241 actctttaga agttatagag gcaattgaga cattaaaagg aaatggacct aaagaccttg
    48301 ttgagctatg cgaagtatta ggagcatata tgttggtttt atcaaaggta gcacatgatt
    48361 ttgaagatgg tgttgaaaaa ataagagaat caataaaatc tggtactgct atacaaaaac
    48421 tcaaagagtt tatagagaat caaggtggta ataaagatgt agtagatgat tattctttat
    48481 tcaaaaaatc aaagtataaa tatccagtta aatcaactaa atcaggttat ataagtaaaa
    48541 ttaaagctga agatgttggt gtatcggcta tgattcttgg agcaggtaga gaaactaaag
    48601 atgatatttt agatttatca gcagggataa ttttagaaaa gaaagtagga gattatgtaa
    48661 atgaaggtga agttttagct tacatgtatt ataatgatga acaaaagctt tcattggcaa
    48721 agggaagatt tattgattcc tactctattg tagattcaaa aatagaaaaa aataaattaa
    48781 tatatggtat tgtaactaaa gaagagatta aaaagtttta gaaaatttt
//
LOCUS       DAFKMX010000005        47974 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFKMX010000005 DAFKMX010000000
VERSION     DAFKMX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.005"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..931)
                     /locus_tag="LZB08_000230"
     CDS             complement(<1..931)
                     /locus_tag="LZB08_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBM7313751.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVI"
     gene            1165..1617
                     /locus_tag="LZB08_000231"
     CDS             1165..1617
                     /locus_tag="LZB08_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7313752.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYIINKMKEE"
     gene            1620..1928
                     /locus_tag="LZB08_000232"
     CDS             1620..1928
                     /locus_tag="LZB08_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBM7313753.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            2042..3148
                     /locus_tag="LZB08_000233"
     CDS             2042..3148
                     /locus_tag="LZB08_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBM7313754.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            3223..5901
                     /gene="mngB"
                     /locus_tag="LZB08_000234"
     CDS             3223..5901
                     /gene="mngB"
                     /locus_tag="LZB08_000234"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBM7313755.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(6218..8551)
                     /locus_tag="LZB08_000235"
     CDS             complement(6218..8551)
                     /locus_tag="LZB08_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7313756.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(8526..9242)
                     /locus_tag="LZB08_000236"
     CDS             complement(8526..9242)
                     /locus_tag="LZB08_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7313757.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(9656..10312)
                     /locus_tag="LZB08_000237"
     CDS             complement(9656..10312)
                     /locus_tag="LZB08_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7313758.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(10367..10996)
                     /locus_tag="LZB08_000238"
     CDS             complement(10367..10996)
                     /locus_tag="LZB08_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBM7313759.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(11127..11768)
                     /locus_tag="LZB08_000239"
     CDS             complement(11127..11768)
                     /locus_tag="LZB08_000239"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBM7313760.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(11777..12448)
                     /locus_tag="LZB08_000240"
     CDS             complement(11777..12448)
                     /locus_tag="LZB08_000240"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBM7313761.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(12453..13337)
                     /gene="rsgA"
                     /locus_tag="LZB08_000241"
     CDS             complement(12453..13337)
                     /gene="rsgA"
                     /locus_tag="LZB08_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBM7313762.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(13435..14172)
                     /locus_tag="LZB08_000242"
     CDS             complement(13435..14172)
                     /locus_tag="LZB08_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBM7313763.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(14280..16295)
                     /gene="pknB"
                     /locus_tag="LZB08_000243"
     CDS             complement(14280..16295)
                     /gene="pknB"
                     /locus_tag="LZB08_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBM7313764.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(16312..17064)
                     /locus_tag="LZB08_000244"
     CDS             complement(16312..17064)
                     /locus_tag="LZB08_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBM7313765.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(17084..18115)
                     /gene="rlmN"
                     /locus_tag="LZB08_000245"
     CDS             complement(17084..18115)
                     /gene="rlmN"
                     /locus_tag="LZB08_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBM7313766.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(18123..19448)
                     /gene="rsmB"
                     /locus_tag="LZB08_000246"
     CDS             complement(18123..19448)
                     /gene="rsmB"
                     /locus_tag="LZB08_000246"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBM7313767.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(19510..20211)
                     /locus_tag="LZB08_000247"
     CDS             complement(19510..20211)
                     /locus_tag="LZB08_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBM7313768.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(20280..21032)
                     /locus_tag="LZB08_000248"
     CDS             complement(20280..21032)
                     /locus_tag="LZB08_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBM7313769.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(21042..21971)
                     /locus_tag="LZB08_000249"
     CDS             complement(21042..21971)
                     /locus_tag="LZB08_000249"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBM7313770.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(21986..22426)
                     /gene="def"
                     /locus_tag="LZB08_000250"
     CDS             complement(21986..22426)
                     /gene="def"
                     /locus_tag="LZB08_000250"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBM7313771.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(22444..24969)
                     /gene="priA"
                     /locus_tag="LZB08_000251"
     CDS             complement(22444..24969)
                     /gene="priA"
                     /locus_tag="LZB08_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBM7313772.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPDIEVVDMKDELDKGNRSIFSL
                     KLQKEIHYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(25208..26407)
                     /gene="coaBC"
                     /locus_tag="LZB08_000252"
     CDS             complement(25208..26407)
                     /gene="coaBC"
                     /locus_tag="LZB08_000252"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBM7313773.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(26412..26678)
                     /locus_tag="LZB08_000253"
     CDS             complement(26412..26678)
                     /locus_tag="LZB08_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBM7313774.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(26681..27298)
                     /gene="gmk"
                     /locus_tag="LZB08_000254"
     CDS             complement(26681..27298)
                     /gene="gmk"
                     /locus_tag="LZB08_000254"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBM7313775.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(27328..28209)
                     /locus_tag="LZB08_000255"
     CDS             complement(27328..28209)
                     /locus_tag="LZB08_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBM7313776.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(28213..29043)
                     /locus_tag="LZB08_000256"
     CDS             complement(28213..29043)
                     /locus_tag="LZB08_000256"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBM7313777.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(29330..30364)
                     /gene="ltaE"
                     /locus_tag="LZB08_000257"
     CDS             complement(29330..30364)
                     /gene="ltaE"
                     /locus_tag="LZB08_000257"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBM7313778.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            30660..32435
                     /locus_tag="LZB08_000258"
     CDS             30660..32435
                     /locus_tag="LZB08_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBM7313779.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDNISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(32650..34302)
                     /locus_tag="LZB08_000259"
     CDS             complement(32650..34302)
                     /locus_tag="LZB08_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBM7313780.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCGHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEAKNKPKVSKEMNIK
                     IQDGMDIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(34601..35812)
                     /locus_tag="LZB08_000260"
     CDS             complement(34601..35812)
                     /locus_tag="LZB08_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBM7313781.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFI
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(36096..36629)
                     /gene="pyrR"
                     /locus_tag="LZB08_000261"
     CDS             complement(36096..36629)
                     /gene="pyrR"
                     /locus_tag="LZB08_000261"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBM7313782.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(36989..37936)
                     /locus_tag="LZB08_000262"
     CDS             complement(36989..37936)
                     /locus_tag="LZB08_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBM7313783.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(37905..38351)
                     /gene="lspA"
                     /locus_tag="LZB08_000263"
     CDS             complement(37905..38351)
                     /gene="lspA"
                     /locus_tag="LZB08_000263"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBM7313784.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(38661..39440)
                     /locus_tag="LZB08_000264"
     CDS             complement(38661..39440)
                     /locus_tag="LZB08_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7313785.1"
                     /translation="MKKIIWGICICLLSIIGVYTYSHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(39495..39977)
                     /locus_tag="LZB08_000265"
     CDS             complement(39495..39977)
                     /locus_tag="LZB08_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBM7313786.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENILLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(40243..41664)
                     /locus_tag="LZB08_000266"
     CDS             complement(40243..41664)
                     /locus_tag="LZB08_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBM7313787.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKQQNNHKLAS"
     gene            complement(41810..42580)
                     /locus_tag="LZB08_000267"
     CDS             complement(41810..42580)
                     /locus_tag="LZB08_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7313788.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(42585..43787)
                     /locus_tag="LZB08_000268"
     CDS             complement(42585..43787)
                     /locus_tag="LZB08_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBM7313789.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DTGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            complement(44375..45376)
                     /gene="trpS"
                     /locus_tag="LZB08_000269"
     CDS             complement(44375..45376)
                     /gene="trpS"
                     /locus_tag="LZB08_000269"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBM7313790.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(45399..46097)
                     /locus_tag="LZB08_000270"
     CDS             complement(45399..46097)
                     /locus_tag="LZB08_000270"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBM7313791.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(46140..46380)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46471..47874)
                     /locus_tag="LZB08_000271"
     CDS             complement(46471..47874)
                     /locus_tag="LZB08_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7313792.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
BASE COUNT        15839 a         8385 c         5164 g        18586 t
ORIGIN      
        1 tgattacttc taaaatttct tcaacatact ctataatttt agtatctacc ctattggaaa
       61 cgttatccaa aagagaactt ttagttataa aatgattata tagataaact ctgtctgcct
      121 ttgaaataat tatagtatat ttactctcaa tataattaaa tatattttca acagttgtat
      181 tcgattgaaa atctgttaaa acaacatcta aaacattatc taagcagtaa ccatcaagta
      241 ttctacttat agtaatagct aaatggataa taaagttttt tagattaaaa tctaggaaat
      301 tgatattaaa tttattaatt tcacttaaca ctaccctttg aagctctatt aaatctacat
      361 tattaaataa ttctatttct ctatctgtaa ttccaattat atagttttca aaattcctat
      421 ttatcatatt attagaaata catcttcgaa tatctatttc cttaccttca actttaactc
      481 cataatatgg cttagaaact aatattaaat tatactttga caaaataggc tttattcttt
      541 ttatgtcatt tagtattgta aatttactca catacaagct attagctaaa tcttctagtt
      601 ttatatatgt attgttatag agaatatatt ttaaaatata ttgatttctt tctataggag
      661 aatctggtat ttcacttgaa tccataatat catcagatat taatgctaga tactgttggt
      721 ataatgctaa attaagaata tctatataat atccttctcc tttttttatt ttaataatag
      781 caccattttt ctctaaagtt ccatttatag aagtaatatc atttcttata gttctctcac
      841 tcacttttaa atgattactt aaagttttac aagtaatttt atcatcactt tcatttaata
      901 atttaaacaa cgtgagcaat ctcttgtgca taaatatccc cccttataga atttcttcta
      961 aattatacta tttacactca tataatacaa ttactctaat aaaattgtat tatcataatt
     1021 tatttccact tctatatgga aattagttat tatcataata taaatttcca tgtcgaagtg
     1081 gaaattagtt atatttaatt taattaggat aatgattaca atataaatat aagttaagac
     1141 attatgaatg gagtggaaaa atatatggta ctacaagaaa aatatatatt tatagataaa
     1201 gacttaaaaa gcaaagaaga agtattaaat tttattgcag atagtgctta tatactaggt
     1261 attacaacta ataaagaggc tcttttaaaa gaccttctaa aaagagaaaa tgaatttcca
     1321 acaggaataa aagatggatt tgcgatacct catacaaaaa gtgtaaatgt aaaggaagct
     1381 tccatactct atattaaaac aactaacgag ttagagtgga aaactttaga tgatagtaag
     1441 gttagatatg tatttagttt gttagcccca cataaaaatg aaggaaatat acatttgaaa
     1501 actctatcta aattagccac atgtttaatg gaggatgatt ttacaagtga agttaaaaat
     1561 gctagtgatg caagtcattt agtaaaatat ataattaata aaatgaagga ggaataatta
     1621 tgaaaatagt agcaataaca tcatgtatag caggattagc acacacacct atggcagcaa
     1681 aagctttaga acaagctgca tcaaaaatgg gtcatgaaat caaagtagaa caacaaggtg
     1741 ctatgggacg agtaaatact ataacaaaag aagaagcaca agatgcggac tttatactta
     1801 tagcatctga ccaaactata tctggaatgg aaagatttga tggtaaaaaa gtgataaaag
     1861 tcaaaatagg tatagcgtta aaaaaaccag aagctgtctt aacaaagtgt atagaagcta
     1921 tttcttaata aaaaaatctc actctaatat agaatgagaa aaatcaaaaa ttaaaaaatt
     1981 gtctaatgta attataacat attatcaaaa aatataacaa acttatatag gaggattaaa
     2041 aatgagtaaa aaaaataaat ctcttttaag tgaacttaag ggacacttaa tgacaggtat
     2101 ctcatatatg ttgccacttg taataggctc atctttagtt gtagctatcc caaagcttac
     2161 agctttagca atgggtataa caagtttaga tgcttataaa aatgtatctg gtttttatca
     2221 tattctttac ttaatggaac aagttggttg gactggcata ggtctattaa atacggtatt
     2281 agcaggattt atagcatttt caataggtga gaaaccagct atgggtgcag gttttattgg
     2341 tgggctcata gcaagtaata ctaatgcagg attcttagga gcagtaatag caggtttctt
     2401 tgctgggtat gtttgtaagt ttttaaagaa aaaaattaaa atatctgggt cagcatcagg
     2461 aatgatgcca cttataataa tgccacttat aacagttgga ttaactggtt ttttaatgag
     2521 tgttgtatta gcaggacctt taggaaacat aaatacttca ttaaatgctt gggtagctga
     2581 aatgtgtcaa aatggaacaa attcggtggt attggcttta attttaggcg caatgatagg
     2641 attcgattta ggtggaccag taaataaagc agcttggatg gcaggaaatg cattattgtt
     2701 agagggaatc tatctaccag cgatattggt aaactgcgca atagttatac ctccactagg
     2761 atatggttta gctacattca taagaaaatc aaagttttcc aatgcattag ctgaaacagg
     2821 aaaaggcaat atagttatgg gtataattgg aataacagaa ggtgcaattc ctttcacttt
     2881 aacaaatcca ttaaaattag ttcctgttaa tatgatagga tgcgctttgg gtgtaggatt
     2941 atcagctttg ttaggtgtac atgctataat gcctccagtt ggtggattat atggatttat
     3001 atcaataggt tctggatggg cttacttagt aggtgcaata tttggagctt tagtaatagc
     3061 aatattatca acaactttag tagacttcaa tgaagatgaa actacggaaa atacagaatt
     3121 tgatgaaata gagttagata taatgtaaat taaaaactta attttagata attgagaatt
     3181 gagtgtactc tcaattctca attaaatttt gggaggattt ttatgaaaaa gacaaaagta
     3241 catgtagttc cacacagtca ttgggataga gaatggtatt ttacaacatc aaggtcaaaa
     3301 atatatttaa tgaaagattt tcaagatata ttagacatat tggaagaaaa agaagacttt
     3361 aaatacttta ctatggatgc acaagcttct ctcttagatg attatttaaa atggagacct
     3421 caggataaat atagaataag agatttagtt caaagaagaa gattagttat tggaccttgg
     3481 tatactcaaa cagaccaatt ggttatatca ggagaatcta taattagaaa cctttactat
     3541 ggaatagata gatgcaatga atttggtgaa cctatgaggg ttggatatgt accagattct
     3601 tttgggcaat cagctcaaat gccacaaata tacagaggtt ttggaataga tagttcatta
     3661 ttttggcgtg gagtttctga tgatatggta gatacaacag agtttatatg gaaaggttct
     3721 gatggttcaa aagtattagc agttcaaata ccatttggtt attactatgg aggtaatata
     3781 ccagaagatg attcagattt aaaagaatat ttaaaagata taattggaaa acttaaggtt
     3841 aaagcttcta ctaataacgt atattttcca aatggatttg accaagcacc aataagaaaa
     3901 aaccttcctg atatattaaa aaaagctaat gaaatagata atgaaaatga atacgaaata
     3961 agtgatatag aaaactatat agattcagta agaaatgaaa gaaatgaatt tacagagctt
     4021 aaaggtgaat ttttaaatgc taaacatatg agaatacaca aatccatatt ctcctcaagg
     4081 tcagacctta aaataatgaa taacaaatta gaaaattata ttacaaatgt acttgaacca
     4141 ttattaacat taagttattc acttggaaat gaataccctc atctagttat aaaagatata
     4201 tggaaactta tgtttgaaaa tgcagctcat gacagcatag gttcttgtaa tagtgacaca
     4261 acaaatgagg atgtatacac aagatacaaa caagccagag atttgagtat aaatttatta
     4321 gatttgcata tgagacttat aagtactcaa atcaaaaata ataatgaaga aataacatta
     4381 acagtattca atggattccc agaagtcaga aatgaagttg tagaatttga tacttatatt
     4441 ccaggtcaag attttgtgat aaagaataaa aatggagaaa ttcttaatta tataataaaa
     4501 gaaaaagaag atattacaaa ttatatatta actcaaacaa taagattaaa cccaagtaaa
     4561 aaaatatata tacccaaaac tgtatataga gcaaaaattg cactttcagt aaaagatata
     4621 ccaccaatgg gatatactca actagttttt gaattggata ctaaaaataa atgtgaaata
     4681 gaaacaagta attgcaaaga aatagaaaac aaatactaca aaataacagc caataaaaat
     4741 ggttcattag aaatattaga taaagaatct aataagactt ataaaaacca agctgtaatt
     4801 gaagataatg gagatgatgg agattcatac aactattctc ctccaagaaa agatatttat
     4861 gtttcatctt tagattctgt atcttcagta gaagtgctaa aatcaaacat tcaagaagaa
     4921 atgatactta aatattcatt gaccatacca gcttcactgg aagaaagggc tataggtaaa
     4981 gtttctgtga aaatgccagt aacaatgaaa attaccttag aacctaaaga acaaataatt
     5041 aagtttaatg ttaatataaa aaataaccaa gcactatctc atagagtaag agttttattt
     5101 aatacagaaa tagcatctaa gttttctata gcagatcaac aatttggaat aatacaaaga
     5161 ccaacagtat tagaaaaaga ccttgcattg tggaaaagag acaattactc atggcaagaa
     5221 aaacctataa ctatcgaacc aatgcaaagt tttgttactt tagaggatgg acaaagagga
     5281 atagcattaa taactggttg tgtaagagaa tatcaaatag ttggtgataa tttagataca
     5341 atagcattaa cactattcag gtcatttgga tatatgggaa gagaaaattt actttataga
     5401 ccaggaagag cttctggaga aaaaataata gcaacaccag atgcacaatt acttaaagag
     5461 ttaaactttg actttggtct ttacatatat aacaataaat ttgatgaagc aaatgtagct
     5521 aatacagcta agagattctt aacacctata caaatatatg aatatgctga ttttttaaat
     5581 ggtagactta tatttgcatt caatgacgaa aaacaaatat atgaaaatga atatagtttg
     5641 atgaatgtaa ataattcaaa tttcacagta agtgcaataa agaaagaaga aaaaggagat
     5701 ggaattgtag ttagaatctt caatggtatg aaaaatgagg atgctaaggg aagtcttaac
     5761 atcaataaaa atgtcaatga tgcttatagt gcaatgctcg acgaaatcta tgataatact
     5821 agcaagctta aagtaaatac taaaactgta gaggtaaatt ctttatcaca ttgtaaagta
     5881 caaacaatag taattaaata atataaaata gcttatacaa gatttcaaaa taaattgcta
     5941 gtactatgtt aatagtaaag ccagatatta tgctatttat aagctctgta acactaaata
     6001 acattttgta aaataaaaag gtgaaccttg tatattagga ttcacctttt ttaaataaat
     6061 caacattctg ttttaaaata acatttataa gctgttttga aataatgctt acaaattatt
     6121 ttaaaacaat acctataaat ttttatttaa agaatacaca taaatatttc cagactcttt
     6181 tatcctatag actttataat tattaaagaa gtctaatcta atttttgtta aaagtaattg
     6241 taactttgaa taaatcacca tccacactta tattcatctt accaccctgt aattctataa
     6301 aactttttgc tattgctaaa ccaagaccag aaccttctgt gttacgtgac ttatctcctc
     6361 ttacaaatct ctccattata tcatctacac taaaatcaat ctcagatgca gacatatttt
     6421 taagcataat atttacttta ttatctgttt ctaaaatatc aatatacact cttgaatttg
     6481 gcattgcata tttgcttata tttattataa gattttcaaa tactctaaat gtacgctgac
     6541 tatctaatgg taatattatt ttttcattag aaaagttatt ctttactatt agagatgaat
     6601 ctgcaagttt gtcatctaat tctaaaagtg tctgcttcat tagagataca atatccacat
     6661 ttactatatt caaataaaca tctccactat ttgccttgct tacttcaaac aaatcttcta
     6721 ttagatgttg caatctttgt gactttctgt ctaatgtatc aatatacaac ttacgatttt
     6781 catctgttat actttcatct tttaataaat ctatataagt gattatagat gttagaggtg
     6841 tttttaagtc atgtgataca tttgatataa gctctgtttt catcttttga ctcttaacct
     6901 cttcgtcaac agctttttta aatccagttt gaatatttcc taattcatct tttataggat
     6961 taaaaaatcc taaatctttt tccatattta catctaagtt tccatctgaa attttctttg
     7021 taacatctaa aagttgatta tattcatata ttgtcttttt agagtaatct gacagtatct
     7081 taaataatac tactgaatat attaatgcga gtattattcc aaagaaccac atactacaca
     7141 ttataactat aatcaataaa tttattccca aaattgtaac tatagtttta tttggctttt
     7201 cttttagatt tattgttcta atttgattaa taactgtttt tcctgctttt tttagccata
     7261 caaaaaacat tcctgtcaca gtatgttttg taaaatactc tcttattcca acatttaaaa
     7321 tgtgttttaa aagtattata aatacaaata ttattgagaa tattacaaac caataagtta
     7381 aattcattaa acttaccaat atactagcca ttttagcact tatgtcattg tttataaggt
     7441 actctacata attcccactc aaggtattac ttattataac agtaggatat aaatctacaa
     7501 gcccctgtat aatagcaaga gcaataaaga gtatctctaa cggtattctt gataatgtat
     7561 caaatcctag caactcttta gattttctat atggaaatgc taaagctaca ataacaacta
     7621 tagctaatat aatttttaaa atcattccag aaatcttata ataagaatta ttttctacat
     7681 tgcttatata tgaaattata ccatcatcaa aataagtatt tccataacca tactctacac
     7741 tatcaccttt acttattact ttaggtacag catatactaa tgttatattt tttattggag
     7801 ataacttata tgaaattgaa tttcctccta aacttgtatc cattaaatct tctcttgctt
     7861 tagataacaa catttgatta ataatttttt gatttactcc ataagaatcc acaatattta
     7921 cttttccatc tttatcaaaa ttcattacaa cgtagaatct atagttttct tttatatctg
     7981 taatctgctt ttgtctttcc tcatcattca tttctgtatt ttttgactga ttttcatcag
     8041 aattttcttt tgaagcatca ttttttgttg tatcaagtac tcctaattct ttattatcat
     8101 taaaaaattc aaaattgctt tctttatcaa ctaaatagta ctttaaattc tttcctgacg
     8161 ctttaatgaa atgctcccaa ttttcaaagt tttcattaaa atctgcatta atactatcat
     8221 ctgtatagta agtttctgaa ccatcataag tatctttttc tttacttaca ttttttatat
     8281 agttcatagt ttcttcattg ataaatagct ttgatggttg tatcatctta ccttcttgtt
     8341 cttccttcat attatgatat aaaacataac ttcctttata tatactatct acaaagcttt
     8401 cctgttcaaa tggattctct gctccacctt tagcacttgc ttgtatagta gaatattgac
     8461 taatcatacc taatgatgct gataacataa gtataaaaac tataatagta actatatatt
     8521 tatctttaaa ttttttcaat tttgtatcca acaccccaca ccacctttaa atatttagga
     8581 ttttttgggt caatttctat cttttccctt atattcctca catgtaccat aactgtatct
     8641 gtatttacag cattctcatt ccaaactctt tcatatattt catcagcaga aaaaactctt
     8701 cctgcatttc tcataagtaa acttagtatt ttaaattcta taggagttgt ttttatatac
     8761 tttccatcaa tactaacttc ttttgtattt tcatttaatt ctaatccgcc tattgcaaac
     8821 acactatcgt cactttctac cttttgctct ttatttccaa ccatgtttag atacttagtg
     8881 taccttctaa gttgtgaatt aactctagcc aatagttcta atggcttaaa aggttttgtt
     8941 acataatcgt ctgctcctat attaagcccc attattttgt caacttcctc tgattttgca
     9001 gatagcatta ttactggaaa attatagttt tctctaagct tcattgtaag tgttatacca
     9061 tccattttag gcatcattat atcaactata gctaaatgta tttcttggtt ttctataacc
     9121 ttaagacctt ctaatccatt gtatccctta aaaacattat atccttgatt taatagatat
     9181 atttctatag catctgctat ctccttttca tcttctacca ccaaaatatt atatgaattc
     9241 attctaatct cctttatatt aatttataaa ttttctttac atatatgatt atacaatatg
     9301 atagaagttt ttccatttat atatatattt atatcttttg ttctggttat ttgtatttaa
     9361 tgtccatctt agaaacctcc ttctagttta aatttaatat tttttcattt tgttttatat
     9421 acttaatatt aaacttaaaa acttaagata ttacatatac ttttctaaag attttctaaa
     9481 gatttaaata tatttaaaat taatatacaa aaaaagtagt tcttattgta ttaattaaat
     9541 caatgatttg aaatataaaa ttaatatcaa taaaacctac tttttaattt tttaaatatt
     9601 tatttttaat ttattttgtt tttaatttac ttcgatttta aaatgtcctt gacttttatt
     9661 ttataaatgt acatatttct tctactggtt ttttagatgg actcttcaaa ttgtcctctg
     9721 gcactccaag agctaacatt gcacctaaaa aatactctcc cttttcaaaa ccagtttctg
     9781 cttctaaaac agcttctatc tcatctgctg catagttttg acttgtcaac caacaagaac
     9841 catatccaag ttcaattgct gatagtgtaa agttttcaat tgcagcacct agactctgca
     9901 tacctggatt tcttataaat aatttatcta tagtctcctt aggtgcatct ataagctcta
     9961 attcataata tcctgatgga ttatatacct ttgtaaaaac taatactaaa actggagctt
    10021 ttaagtaaaa tagtgtaaaa tttttaacaa attttctaaa tctattagcc ttatccactg
    10081 atactttatc catttctaca agtatttctt gttgcttttt agttatcaca tctgctatct
    10141 tttccattaa atcacggcgt tttataacaa caaaatgcca gttttgaata ttcttgcctg
    10201 atggtgctgc acctgctgct tttatcattt ttaaaatatc ttcatctgat acatcttgat
    10261 ttttaaactt tcttacactt tgtcttttaa aaatagtatc ttgtaattcc atttttaaat
    10321 ccccccataa cttttttatt attttaatat aatttttaaa ctcatcttat tttaatatat
    10381 catgttctaa caaatcttta aatgaatcta taaaataatc atatacatct acattcccaa
    10441 acccatattt ggcatatata aaaggtatac ctacaaactt agcactattt gcatcacctt
    10501 gcgtatcacc aacatagatt ggatttatta atttatttct ttcaattata agtttattat
    10561 tttctccttt atgcaaacca gtattaccag gacattcaaa atctataaaa tactgttcta
    10621 gtttatgtgc ttttaaaaaa gattctatat atccagcttg acaattactt actataaata
    10681 atttatattt tttagatagt aatttaagag tatcttctat atcatcaaat aatttagctc
    10741 cattttcaga taaatagtca cattctttca aagaacattc attcaataaa ttcattcttt
    10801 cttcttcact taaatttgga aataaacgat ttgctatttc ctcaaaagaa agccccataa
    10861 gacttctaaa taaatctttt gtgacactta attctatttc cttatgctca cttaaaacat
    10921 cctgccagac tttgcatact tcttcagttg aatcccacaa tgttccatct aaatcaaaaa
    10981 taatactatc taccacaaaa aatccccctt atgtcaaagt ttattatata tttataatta
    11041 tatactaaaa agcagataca atcataaata tattttactt gattgtatct gctctacaat
    11101 ataaaaccaa ttgtcaaaat caaattttat aaatttctaa ctacaagtac acagccttgc
    11161 tctactttaa tcttacaaga attactcaac ataacatttg atattccaat aggtacagag
    11221 tatttcatat aataattatc gagagggtat tctaagccag taagtgttac acccttagca
    11281 tcaccattta tagctattac agaaatagtg tcccctgggt ttccatacaa tgaaatttcc
    11341 tcattttcaa ctatatacat ctcttcttta tcagaaagta tcctagaata tataccatct
    11401 tcttttaaat aatataatag ctttatattt gcaagagtgt ggtcaatcct tccccctaaa
    11461 gcaccaaaaa aatcaatatg atttgcttta agtgtcttag ctaaaaaaag acaaagctct
    11521 gtatcagtct catctttttt agatggaaat ttttcaaatt taacaccttt atttttatag
    11581 aagtttatct tttcttcttc tactgaatct aaatctccta atatataatc tggcattatt
    11641 tccattttat aagtgtgatt tgcccctcca tctgcacata ttatgcagtc ataacattcc
    11701 ttaattataa tatctcttgt aacttcgtaa tcttcaattt cgccatttaa aacaatacag
    11761 attttcatta ttaaatttaa actaattctt caagagaagc attctttcta aataaattaa
    11821 ctgtttcttg aatgtcatca cttccaaata ttgcagaacc tgctactatt acattagctc
    11881 ctgcttgtac aacttcggct acgtttttag ggcttatacc accatcaact tctatatcaa
    11941 tgtttaatcc ttgactatct ataatagctt ttaactcttt aatcttatct aaaacacaag
    12001 gaataaatga ttgtcctcca aatccagggt taacagacat taataatacc atatctaaat
    12061 caggtagtac atgtttaatc gtatctactg gtgtagctgg attaagtact acaccagctt
    12121 ttattccatg tgattttata ttttgtatag ttctatgtag atgaacacaa gcctcctggt
    12181 gaacagttat tatatcacat cctgctttta caaattcttc aatatactgg tctggatttt
    12241 ctatcataag atgagcatca aataccatat ttatattttc tttctttaaa cttttgacta
    12301 caagtggtcc taatgtaatg tttggtacaa agtgtccatc cattacatcg atatgaagat
    12361 attcacatcc tgcactttct acttttctta catcttctaa taattttgca aaatctgctg
    12421 ataatattga tggtgctaat ttaatcatgt ttctaatacc tcctactatt attttgtctt
    12481 atttcatgta gcagctgtat ataactatca tatctttctt tagatatttc tccatttgta
    12541 acagcttctt ttatagcaca attaggttca ttttcatgaa tacactttga accaaactta
    12601 caatcattaa atttatcaaa ttctataaaa taatctttaa gttcaacttc ctctatatct
    12661 tcaagggcaa gggaactaaa acctggtgta tctgcaacca ttccaccaaa ttctaatttt
    12721 aaaagttcag cgtgacgagt agtatgtttt ccccttttta ttttatcact tacaactcct
    12781 gtttgaagtt taaaattttc atctatttca tttaataagc ttgattttcc aactccagat
    12841 ggtcctgcaa acactactac attttctttt aattcttctt ttactttatc tatatttaac
    12901 ttagtaatat tgcttacagg aatgacttta tagccactaa gttcatatat atttttaaca
    12961 gtttctaatg tatcattatc atctaaatct gcttttgtaa ggattattac agtttctaaa
    13021 ttttcttttt ctgccaaaac tataaatcta tctaaaagag ataagtttgg tttaggattt
    13081 tttattgcga atactattaa tgctttatct acatttgcaa taggaggtct tataagttct
    13141 gtatctctac tgtcgatttc ttctaagata cccttttttt cattttcatc aacaatgctt
    13201 attttgactc tatcaccaac caaaggtgtt attttctgct ttctaaatat ccctctagcc
    13261 ttacattcat aaataccatt ataagtatct acataataaa atccactaat cccctttata
    13321 atttttcctt ctagcatcaa atgcctccct ttataaaatt tatataaaca catactgttt
    13381 atgagtagta tatcgtgtta ttctttccta tttttatata ataccacatt ttttttaaat
    13441 aacaaatcct ccgtttggac taattacctg accagtgata aaatttgatt tgtctgatgc
    13501 caagaaaata gcacaatttg ctatatcttc tggagttcct agcctcatta gaggggtttc
    13561 ttccacaaga gcgtctatat cttcctcatt atatccagat aacatgtcag tattaatgac
    13621 acctggagct atgctattta ctctaatatt tgatggtcct acttctttag ctaatgcctt
    13681 tgtcatacct attatgcctg cttttgttat agaataatgc acttcacaag acgcacctga
    13741 tataccccag attgaagata tattaattat atttcctttt ttttctgata tcatgtattt
    13801 taaagcaact tgtgaacaat aaaagcttcc ttttaaatta atattcatca tattatccca
    13861 atcttcatca gttatatctg taaacaactt atcttgactt acaccagcat tgtttacaag
    13921 tacatccaat cctccaaatt cttttataca ataatctacc atatcctcta catcttctct
    13981 atttgaaata tcagccttaa aaagttttac agaaaaattt ttttcattta atatagtgta
    14041 caactcttta gcttcatttt ctgacttatt aaaatttact aatacattgt aaccttcttt
    14101 tgcaaaagct ttagacatag ctttaccaat ccctctagct cctcctgtaa ttaaaactgt
    14161 tttcttcttc ataaatactt ttctccttat attataacca attcagtatt ttatacaaaa
    14221 caaactgccc tttaaagaaa atattttcta taaaaggaca gctcttattt tattaatatt
    14281 tattctgttt taccaatgtt tgcgccagtt cctgatgaag ttcctccatt atctcctgag
    14341 tttgagcctc ctgaactatc tccattgtct cctgagtttg aacctcctga acttcctcca
    14401 ttgtctcctg agtttgagcc tcctgaactt cctccattat ctcctgagtt tgagcctcct
    14461 gagtttccac cactatttcc tgagtttgaa cctcctgagt tacttccatt attgcctgag
    14521 tttccaccac tatttcctga atttgagcct cctgaatttc caccactgtt tcctgagttt
    14581 gaaccaggtt ctgttggtgt attattctct ggtgttgttg gttttttatc tggtactgtt
    14641 ggagtctgtg tatttgattt ttctgaacct ttgcttacat ataggtttac ctcatcacct
    14701 tcctgagctc ttgatgagcc agcacccgga ctctgactta aaactgtacc ttctttatat
    14761 attgagctgt attcatactt aacagttccc acttttaagt tattttcttc aataagttgt
    14821 tttgcattac tcaaagtaag tcccactaca ttaggaacag atgcctgact tgaaccttta
    14881 cttacgacaa catttatagt atcacccttt ttgacatttg tacttccacc ttcaggtgtc
    14941 tgtgatatta tacaattttc atcaacttca ctattatact cttcactttt tacactcagg
    15001 tataatcctt ctttttcaac agcactttga gcttcttcta atgtcatatt tacaagatta
    15061 ggaacagtca aagatttatt gccaaaccct ccagcaaata aaaacttata tgctaagaat
    15121 acttgagcac acaatatcag tatcaaaaca gccgcaatta cttttaatct tcttctagat
    15181 tttgggcttt cctgtttttt cttctttttc ttagttctct tacttggagt actcttaggt
    15241 ctctgattct ttttagctct cattatctct tcctcttctt catcttcatc atcatcttct
    15301 tcataaaaat catcatagta atcttcatca tcatccaaat cagctacttc aacaggctta
    15361 accacttttt caggagctgg ttttgcaagt gttggattta caactttatt aatttctttt
    15421 tcatcaattt tcttagttgc aaaatcatca tattctttta taaaatctaa atcaatattt
    15481 ttttctatat actctatatc ttctatcaat tcttcagcag tttgatatct atctgcacta
    15541 gatttttctg taagcttttt tattgtagtt cttacacttt gaggaatcct aactttttct
    15601 tctgatgtaa agtctatatc atcattaatg tgttgaagtg ctatagaaat tggactatct
    15661 cccctaaatg gaacttttcc tatcaacatt tcatataaaa ctatacctag agaatacagg
    15721 tcagcattat tagttacaaa tttaccttta gcttgctctg gtgaaaaata atgcactgaa
    15781 cctattatac taccaatatt agtcattgtt gaatttgaaa cagctttggc tataccaaaa
    15841 tcagccactt ttactactct tccttcattt gatatgagaa tattatgagg ttttatatct
    15901 ctatgtataa tccccttttt gtgcgctgca ctaagtgcca tagctatttg ttttgttata
    15961 tctaatgcag tatattcatc taatatacct tcatcttgta taatctcttt tagattcttc
    16021 ccatctacaa actccattac aatgtagtga acttttccat cttcaccaac atcataaaca
    16081 tttactatat ttggatgtga aagacttgct actgcttctg cctctctttt aaactttgtt
    16141 agaaattcat catcatctac aaattcaggt ctaagtactt taagtgcaac agtcctgttt
    16201 aataatctgt ctttagcctc atatacaaag gccattcctc catcaccaat cttcctgatg
    16261 atttcatatc gatttcctaa aattgtatct cccacaatat caccgtccta attatacttt
    16321 tatcactata accgaaacat tatctttacc agaaacatca ttggccatac ttatcaattc
    16381 ttcacttata ttactagttc tctcatattc ataatctaat attaaatctc ttatatcttc
    16441 atcatcaata aacccagtta atccatctgt cgcaagaagt attatatcac tttttttgat
    16501 ttctttctta aaaatatcta caacaaccat atcatctgtt ccaattgctc tagttattct
    16561 attcctctgt ggatgtcttc ttgcctcttc ttctgtaatg acatgggcca tcataagttc
    16621 ttcaaccacg gaatggtcta tagttatttt atcaaatttt tcatctgtta acaaataaca
    16681 tctgctatct ccaacatttg ccacatataa gacattatta tacactatag ccgtaactaa
    16741 tgtagttccc attccttcaa attctatatc ttccatagat tttttgtgca caattgagtt
    16801 tacattatta taagcctgtt ttaatatatc atctatatag tctattttta cattatcatg
    16861 ttgtaataag ttttccttta gaaaatcaat tatattttct actgctaatt tactggcgac
    16921 ttcacctttt ttatgtccac ccattccatc agctatggca aatattccta ttggaccatg
    16981 ttctgtatct ataacttctc cctcacaata gtcttcattg ttttttctta tttttcctat
    17041 atgggaggca caactataaa ccatacgact tcctccttac aaattacttg tattttcttc
    17101 tcaattgccc acaagctcca ctaatatcag aacccataga catcctaact gtggcaggta
    17161 tattgttctt ctccaaacta tccctaaatt tataaataaa tgctttatct ggtttttcgt
    17221 attctctttc ttcaacttta tttattggaa ttaaatttac atgacatagc atacctttta
    17281 ggagcttagc taatgccttt gcttcttttt ctgagtcatt tacccctttt ataagagaat
    17341 attcaaatgt aacccttcta tttgtctttt ttatataata tctacatgca ttcaatattt
    17401 cttcaataga ataagcattt gctacaggca taatttttct tcgttcttca tcatatggtg
    17461 aatgaagtga taaagctaaa tttatagcta tttctaagtc tgccaactca tacattttag
    17521 gtactattcc acaagttgaa agagttatat gtctataccc tatattaaga ccattttttt
    17581 cgttaattat ttttaaaaat tgttttgtat tttcaaagtt atcaagtggc tcaccactcc
    17641 ccatcaatac aagatttgat accctcttac cagtatcttc ttgaatttta ataacttggt
    17701 ctaaaatttc ccatggctct aagtttctaa ttaatccatc cattgtagat gcacaaaaat
    17761 tacatcccat cctacaacct acttggttag aaacacagac agtaactcta ctatcataat
    17821 ccatcatcac agtttctatt atatttccat catctaataa aaacaaatat ttttttgtat
    17881 tatctacctt agattctaac tttaattcta tatctatatg ccctatacat gaaacctctt
    17941 ctaatttatt tctcaaactt tttggtatat tgttcatatc atcaaaagtt tttgcaccct
    18001 tatatatcca agagaatatt tgacttcctc taaagggctt ttcatctata gttttcataa
    18061 attctttaag ttcatcttca gtaaaatttt ttaatactat ctttttcttt tccattctct
    18121 cactacctta ttctctttaa ttttgctatg aaaaaaccat ctataccatg aatattagga
    18181 tatatcttta aatatccttt atcttgatta tctaagtcta catttactgt atcaattgga
    18241 acaaattcaa agttgtgatt ttcttcaaca aaagaagtaa ctatatttat attctccaca
    18301 tcttgaattg tacatgtact atatactaaa gttccaccaa ctttgacata tttagatgca
    18361 ttttctaaaa ttttcttttg tatagaagtg atgtctttta actcttcttc tttcttatac
    18421 ttaatttctg gtttacgtct tattatacca aaaccagagc aaggaacatc tgctaaaaca
    18481 taatcaaatt tacttatact attttcatca atctcagaag catcaaatct ttctacaaac
    18541 acatttttaa gccccaatct atttacagta gagtttataa gctttagttt atgctcaaaa
    18601 atatctctag ctactacttg accagtattg ttcattaaag tagctaagtg agtagatttc
    18661 cctccaggag cactacatac atccaaaaca aatgaacctt cttttggatt aattactttc
    18721 cctactatca tagaacttat atcttgaatt gtaaataaac cagccttaaa taattcattt
    18781 ttttctatat ttttcatttt ttcgacttta atagcttctt ctaccatagg aacttttaaa
    18841 catacaatac ccatttcact taatttttca ataagttctt ctctacttat tttaagtgta
    18901 ttcgttctta tatagatact aggcttttca ttatttgctt ccaacaaatc ttcagcaaat
    18961 tcttgtccaa atttatctat ccagtttttt ataatccaag agttatagga atactttata
    19021 gataagtatt ttacactatc ttcctcagtt atgtccatta tagtctcttt agctcttatt
    19081 tcatttctaa gaatagcatt tatgaaacca gaagattttt tatcatattt cttagataat
    19141 tttacagttt cattaactgc tgcataatct gatatactat ctaaaaataa aatctgatat
    19201 gttcccattc ttaaaaagat tttaacataa gttgacattt tttttacctt tatttttgaa
    19261 agtttattga ttatataatc taaataatat ttattttcta ttactccata aattaactct
    19321 gttgcaagtc ctctatccat atcacttatt tctaaatttt taaaatgctt atttattgca
    19381 atatttgaat aattgtcatt tttttctata tcacataata ctttaaatcc aacttctctt
    19441 gcatccatat aaatttctcc cttttattaa acataaaaaa gggttgtttc taaacaaccc
    19501 actttacttt tagtcatttc ttctttgagc tattagtaac aatctaagta attgaagtat
    19561 tgaaaccaat gctgctgcaa catatgttaa agctgctgct gaaagtactc ttctactttg
    19621 tcttgactca ctttcatcta taatccctaa attcccaagt tgaacgattg ctctacttga
    19681 agcattaaat tctacaggca atgttattat ttggaataac actgaagccg aaaacaatag
    19741 tataccaatt tttaaaaatg gaccagccat aataaatcct aaaaatatta aaaaccaaga
    19801 tatacttgaa gcaaaattca ctactggtac taaactactt cttatttgaa gaggtgcata
    19861 acctttagca tgttgtatgg catgaccaca ctcatgtgca gctactgcaa cagaagttat
    19921 agatgttcca taatatacat cttctgacaa ccttacagcc tttcttcttg ggtcataatg
    19981 gtcacttaga tgccctctaa ccatttctat ctttacatca tacaaaccat ttgaatcaag
    20041 tactcttctt gctgtttgct ctccagtata tcctcgacgt gtatttactc ttaaatactt
    20101 atttgttgtt gaactcactt taaattgtgc atatatagta agtaatatag ctggaattaa
    20161 tacaaccatt gttgggtcaa atccccaaaa accgccatat acagggtaca taaataacac
    20221 tctcctaaaa tatattttta ttatacatcc tcttaatatt cacttaactt aaaaacagtt
    20281 taattacaaa ctaatatatt ttttcctgtt aaaaagccta tagctttttc aatatccatc
    20341 atgtctacat tagtatttac acaaggtcca ttaggtcttt tgttaaatac acctaaaaca
    20401 ggaatttttt taatatcttg tattccagat gtcaaatctc tttcacatgc aacagctaca
    20461 acagctttag gtttagtgtc cattataatt tttctagcta gggttcctcc tgtagctaca
    20521 aatattttta cattagtttt ttcctttagc ttaattaatt ctcctatgtt gcatcttcca
    20581 cattctttac aattatctat atcattagta acttttaatt tacaactatt ctcttgaata
    20641 caatgaggta ttaaaattat tatatcctct ggattaaaat tatatttatt actgtatata
    20701 tattcattat ttaactttat atatattctt ctaatatcac tttttgacat accaaatgat
    20761 gatgcaatga aagatataat aggaaataat ccactgacaa tattaaaatt tattttcatt
    20821 atatttgagt taacatactt tcctttaatt actctataag taactgctgt tgaacatagt
    20881 attataagta ttagtgatat tattataata tttatcatca aggagaatat ttgagttaaa
    20941 cttattataa aaaaattaac aactcctatc aaaattccta atattaaaat caaaatccca
    21001 acacttgtta aatacttctt tatatctgtc atacttttca cctaacccaa tactaaacca
    21061 gtttctatag tattcccttt tatatattca ccaaccaaca ttctcttttt attcggcatc
    21121 tgaatttctt caattaaaag tacattatct atagtactta cttttatccc atctttgctt
    21181 acatcaatta ttgtacctac atcttttgtg cttttttcat ctaacacttt agttttccaa
    21241 actttcatta tgacatcatt ataggtagta taagcacttg gccatggatt tactcctcta
    21301 actaaattgt gtatttctct ggcacttttt gaaaaatcta tgttacctaa tgatttattc
    21361 attattggcg cataggaaaa ttcttcatga ttttgtactt ctcttggagc atttccttct
    21421 tcaattaatc ttaatgtttc ttttaaagtt tctgctccta tattcatcat tttatcatga
    21481 agttctcctg ctgttatatt ttcatcaaga tttacttctg tctttagtat catatctcca
    21541 gtatctaatc cttcatccat atacatagtt gtaacaccag tcttttcttc accatttatt
    21601 attacccaat ttataggtgc cgcacctcta tattttggaa gtaaagaaac atgaacattt
    21661 atacatccaa gtttaggaat ctctaatatt tcttttggaa gtatctgtcc aaaagctaca
    21721 actactatta catctggatt taaagattta attttatcta taaattcttt atctctagcc
    21781 tttactggtt gatatacagg tatattattt tcaattgcca gttcttttac tggactcata
    21841 cctagttttt tacctcttcc ttttggttta tctggttgag ttacaacacc caatatttca
    21901 tacttctcat ctattatttt ttgtaaacat ggaactgcta tatcaggagt tcccataaat
    21961 actattttca tcaaatcaac tcctactact tttctatttt gtcaacaaat aaaattccat
    22021 ctaagtggtc tatctcatga caaaatgctc tagctaaaag ttcttctccc tctaactcta
    22081 ttgtttcacc atttctattt aaagctctga cctttacata atttggtctt ctaacatttc
    22141 ccgcttctcc aacaacactt aaacatcctt cctcatctat ttgctcccca gatgtttcta
    22201 ttatttcagg atttataagt tctataagct cttctccaat atctattact actactcttt
    22261 ttaatattcc aacttgaggt gcagccagtc caacaccatc tgcatcatac atagtttctg
    22321 ccatatcatc taataattgt attatctttt catcaatttt ttcaaccttt tttgattttt
    22381 ttcttaatac tggttctcct atttgaacta tttgtcttaa agccattata attcctccta
    22441 aatttataat acactgtttg gattaatatc tatagataca ttgatattct tatcaaatat
    22501 caaatctcgt tttgttatac atatatattt tattataccc tttaagagat taatttcaat
    22561 attttcatct ttaaagagta tttgccatct ataattttga tttatttttg atattgaaca
    22621 tggatttgga cctaatataa atcctaaatc atttataccc cttttttcta ataaataaat
    22681 caatgatgca tgcatatttt ttatattttt tatcactaat ctttcatctt caccactaac
    22741 aactacactt aacatattat taaaaggaga atatccaaat gcttttctta tttttatctc
    22801 atcttcataa aaaccctcat aatcatattc aatagcatgc ttaatagcat agtgttcagt
    22861 atcataagtc tgaagtacta cttttccctc tttatcagct ctccctgctc ttccagagac
    22921 ttgagttatc aattgaaatg tagtttcaaa acttttaaag tctggaaaat ttaatatcat
    22981 gtctgcagac aaaataccta ctaaagttac attctcaaaa tccagccctt tacttaacat
    23041 ctgtgttcca ataagtacat ctgcttcttt atctttaaat ttatttagta tctcatctaa
    23101 agcacctttt tttgaagttg tatctttatc cattctcaaa gtttttatat ctggaaaaat
    23161 atattttaat tcctcttcaa ttttttgagt tccaactcca aatggcttaa cataagtact
    23221 tttacattca ggacactctt ttggaatttc tttctcatat ccacaatagt ggcatcttcc
    23281 agtattgctt ttcttatgat aagttaaaga tatatcacaa ttttcacatt gaaacacata
    23341 tccacacttt ctacaagata caaaatttgc ataacctctt ctgtttaaaa atagtattac
    23401 ttgattattt tcttcaatcg cataatgtat ttctttttgt aatttcaaac taaatatact
    23461 tctatttcct ttatctaact catctttcat gtctacaact tcaatgtctg gaagaggctt
    23521 atcatttgct ctacttttta tatttataag tttgtattct ccagattttg ccctataata
    23581 ctcttcaatt gagggcgttg ctgaacctaa aataagagtt atattatttt ttaatgccat
    23641 aaatctagca acttctatag cattaaattt tggattcttc tcagacttat atgatgactc
    23701 atgacattca tcaattatta ttaagcctaa ggaattaaaa ggtgcaaata aagctgacct
    23761 agctccaatt aacactctta ctttacctgc tttgacagct ttgtaaacat catgtttttc
    23821 tccttcagat agtttactgt ggaacactcc aaccaaatct ccaaacctac tctttagcct
    23881 atctattgtt tgaggagtta atgctatctc aggtactaag aatatactgt ctaagccttg
    23941 atttagagca aattctatta tttccatgta aacttcagtt tttccacttc cagttactcc
    24001 atgaattaag tatggttttt tatcatctac aaacatattc gactttatct catcaactgc
    24061 ttcttgttgc tcattgttta attttatact ttttagtacg gatttgtaaa tactttttgg
    24121 ctctctataa taatccttaa attctaaggt tattagcttt ttcttactta gagaatttat
    24181 actttgtttt gaagcattta ataaatccaa taaatcattt atctctacat tttcattatt
    24241 tttaagaaaa cttaatatct ctttttgttt acttcccaca tttattttat tttgctctat
    24301 ataatcgtca atcttatcac tttctaaact caaagatata taacatacct tcttttcatt
    24361 tttgtgattt ttatattccc aacataattt tataattcca tttttattca ttgtgtacaa
    24421 agaactattt aagtttggta tatattttaa cttatcaacc ttaattttac ctttactatc
    24481 aatcactttc tctactattt ctctattttt atcacttaaa gttgatattt tttccttcaa
    24541 ttcaatatca tttaatccac ttaaactttc acctaatagg actactttat aattatttaa
    24601 tttgtatccc tttggatata tcaaatttat acattctata taagtacaca aatatctatt
    24661 tttcatccaa taaaccaact ctaaatcttc ttttctaaat atcggatttt catctaatat
    24721 atcaataatt tcttttattt ttattttttc atctaagcta tctgtaaatt gaaatacaaa
    24781 ggcttctgtt ggtttattac cttttccaaa tggtactaat attctgtgcc caatttttat
    24841 tacatcagat aaaaatacag gaatttgata tgtaaataaa ttatctgtat atatagtatt
    24901 acttctaact attacctttg catatttttt cattataaaa cctctactta aatttaatat
    24961 attgttcata ttttaatata ctttacaaga tattaaaatc tcagaattta taagcaattt
    25021 caagttatta atattaactt tattttaata attaactatt aattatattt tatactttat
    25081 gacaaaataa atgctgaaaa ctacaattta actgacattt gcgagttttt actcgctaaa
    25141 attaaattgc agctttcagc cactttagct gtgataaaat acacaaatat catatttaaa
    25201 aaaggtctta tttctcaagc aacgctttaa ctttatctaa aataacattg gctacttctt
    25261 cttttttcat cttaggatac tcagtaatat ttccctctac atctattatt tttacaatat
    25321 tagtatcagc tctaaaacca gcaccttctt tagttaaatc atttgccaca ataaaatcta
    25381 aatttttctt ttttattttt cctttggcat gttctataag gtcattagtt tcagctgcaa
    25441 atccaattag tatcttatct tcttttattt ttcctatttc ataagcaata tcataatttc
    25501 tacttagttc tattgataaa tcatcattct tctttttaat ttttttgtca gaataagttt
    25561 ttggtttata atctgcaact gctgcacttt gaattactat tttattttca cttaaatttg
    25621 atactactgc ttcatacaaa tcttgtgctg actctatttg aacaaatttt ttaagatttt
    25681 gtggaataga aagatttgta ggacctgata ttaatgttac atcagcacct ctagctatag
    25741 cttctcttgc tatagaatat cccattttac cagaagacct attagttaaa tatcttactg
    25801 ggtctatact ctctacagtt ggaccagctg taactatcat actttgacct tttaaatctt
    25861 gctctttagt aagattagtc aaaacttcct ctactattat atctggatta gctagtttac
    25921 ctgttccaat atctccacat gcaagccttc cactttccgg ttcaataaat ttatatccaa
    25981 agctctttaa agtttgtata tttttttgaa caattggatt ttcatacata ttagtattca
    26041 tagctggtgc tataagaacc tgtgcttttg ttgccataac agtagttgtc aacatatcgt
    26101 cagctatacc atttgcaatc ttgcctataa catttgcact tgctggcact attaagaata
    26161 aatctgcttt ttttgctaat gatatatgtt caacttccca agtttttggt tcttcaaaca
    26221 tatcttctac tacataattt tgactcagcg attgaaatgt aagtggttta acaaattctg
    26281 ttgctgattt tgtcattata acatggatat ttgcatttag tttttttaac ttacttacaa
    26341 catcacaagc cttatatgca gctataccgc cacttatgcc aattacaaca gttttatctt
    26401 ttagcatgac cttactcctc ttctgaaatt tgttggtgtt gctcagcatg atgtctagct
    26461 tcttctatct ctatttcctc ttctgtcaat aatctatatg ttatttttcc acttgcaact
    26521 tctttagttg ctacacatac aggcttttcc ttagttttat ttgaaacata tggttcttct
    26581 ccatcaatta attttcttgc tctctttgaa acagtcccta ctaaatagta tctgttgtct
    26641 attttttcta gtacctcatt gatagatggt tttaacataa ttataattcc tccttaaatt
    26701 tctctattat aatattttta tatctgctaa ctttattctt ttcagatgat attatacctt
    26761 ctatttcttt agcagatgtt tttacatctt cattaaatat aaaataatca taatccttaa
    26821 tcattttaat ttcttcatat gcacagctaa atctcttctc tatttcagct tctgtttctg
    26881 tacctcttcc aacaattcta ttttttaatt ctttaagtga tggtggtagt acaaatataa
    26941 atactccctc tgggcaaact tcttttattt gttttgctcc ttgcatttca atctccagta
    27001 atacatcttg tcctttttct aaacattcca ttattgccgc ttttggagtt ccataaaaat
    27061 tatcataaat ttgagcatac tccaaaaact cacctttttc tatcatctct tcaaactttt
    27121 ccttacttat gaagaaataa tttactccat caacttctcc agttctgggt tttcttgtag
    27181 tagctgatac agaaagtttt attgtatcat tttctttaag tagttctttg caaatagtac
    27241 cttttcctgc acctgatggt cctgatacaa ctagcaataa tccttttttt acaaacattt
    27301 aagcattccc cttctaatat tgtattctta ctctatattt tgtatttgtt ctcttatttt
    27361 ttccaattcg ctttttactt ctactactaa attagttata tttatatcag atgacttcga
    27421 acctatagtg ttagtttctc tattcatctc ttgtattaag aaatctattt ttctacctat
    27481 agagtcgtct ttaaatatag tatttttaag ttgccctata tgacttttaa atctaactat
    27541 ttcttctgtt atactgcttt tatctgcata tattgcaact tcttgtgcta atctattctc
    27601 atctattata cttgggtcat ccagtaattc acttattctc aaattcaatt tttctctata
    27661 atcttctaca acactacttg aatatttttc aatctcttct atatgatttt ttaataaatc
    27721 acatctcatg gcaatatctt cagctaattt tttaccttct tcacttctca tttctcttaa
    27781 tttgattaat gcatcttcaa ctgcttgatt caacattgac cacaataaat cttcatcttc
    27841 ttccttttct tctattttca caatatcagg aaactttgca acattcataa cgctaatatc
    27901 atctataata tcaaatttat tttttatttc ttttagtatg tctatatact gagatgctaa
    27961 tccctcatca aaatttaagt ttacatcttc tttgcctaat aagtcaaact ttatgtataa
    28021 atctactctt cctcttttta cataattttt tactaaattt ctaactttat cctctaaaaa
    28081 tgaaattttt cttggaagac gtatgtttat atctgaatac ttatgattta tcgttttaca
    28141 ctctactaaa aagtaataat tatcatcctt gtattctcct cttccaaaac cagtcatact
    28201 tatagccatt aattaaacct ccaaaatacc ttcacatatt tttactgctg gacctgtcat
    28261 ataagagaaa tctcctacat ctattttaac tgttccacct tgagacgtta catttacact
    28321 actttctacc atattaagta aattgcacac tattgctgat gcagtcatac cagtaccaca
    28381 acctaaagta tatccacaac ctctttccca agtataaaca tgtatattat acttatcttc
    28441 tacctttaca aaatttacat tcgttttttc aggaaatatt ttattatttt caatctcttt
    28501 tccatattta caaacatcat ctacatctaa ttcatctact ataactactg tatgaggaac
    28561 ccccatcaac atagaagtaa ctttaagctc cctatctaaa acctttatac tttcttgcac
    28621 aaaacgttct ttatttgtaa gtacaggaac gtccttagct ataaaacttc cttttcccat
    28681 attgactctt atagagttta tttctctatt ggtaagattt acttttatat cttttatacc
    28741 atctaatgta tctactgtaa aactttcttt tttaactata caattatcat aaacaaattt
    28801 acaaaaacat ctaaggccat ttccacacat agctgctctt gaaccatcag aattataata
    28861 taccatttct acatctgcaa cttttgaatc tttcacaact aacaatccat ccgctcctac
    28921 agaaaatctt ctatggcata ctttttttgc taaagcagta taatcagatg gatttatctg
    28981 gtcgaatctt ccatctattg ctataaaatc atttcctatt ccatgtagtt tccaaaattt
    29041 cataagtatc gctccttcgt aaattaacgc ttaaactatt ataacataaa aaaattccca
    29101 taaacttata atttatggga aaagttttgt aatgacaact ataatttgag gatgtgaggg
    29161 agagttgcca ttacaattag ggtaattata ttatgttttt aagctagttc tttctatgtt
    29221 tatatattaa tatttctata tgaattttat gtatgtttta tttaaactgt ctatgaattt
    29281 tttaattaaa catctggtaa ctagctataa tccttctagt agatatctat taacatacac
    29341 ctattaattc tttaagtgtt tttaaaacat agtctaaatc ttccttagta atatctacat
    29401 gagttacaaa tctatattca ccattttcca ttttattaat ttttatattt ctttcaaaaa
    29461 attcttttat taaaacatcc tcttttataa tatcttcact catcttaaaa aataccatac
    29521 ttatatctgg attaacatta tttacttcaa tgccatcaat tttatttaat tcacaagcta
    29581 aatattttgc attttcatgg tcttcattca atcttaaagt cattttttca atggcaatta
    29641 taccagctgc tgcaactata cctacttgtc tcattccccc acccataact ttttgctttc
    29701 ttctagcttt tgctataaat tctttacttc cagcaagtat agaacccatt ggtgcagcta
    29761 aaccttttga cacacagaac ataacactat cacaacactt tgctatttct ttagcttcta
    29821 cacccaaatt taatgctgcg ttaaataatc ttgctccatc taaatgtact ggtaagttat
    29881 gtttctcagc tattttttta atttctctta aattatcaag actcacaact gttccacctg
    29941 aatatgcatt ttctacacat ataagccctg tctgtggttc atgtatgtca tctcttctaa
    30001 ttgccttttc aacaagttca ggatttagag ctccactttc gtcttcatct atagttctaa
    30061 gctgaactcc agctattaca gcaggtgccc caacctcatg agtcacaata tgataacctt
    30121 taccaataat tatttcttgc cctcttaggc aatgagtaaa tagagctaat tgattcccaa
    30181 aagttccact tggaaggaaa atagcatctt cttttccaac tttagctgct gctaattctt
    30241 caagtttatt tacagtaggg tcatctctaa agattgaatc tccaacagga gcctttgcca
    30301 tagcttctct catttcgtca gtaggcatag taacagtatc acttcttaaa tcgataaatc
    30361 tcattttttc aaccttcttt catatttata tactatagaa ccttaaaatt gattttttct
    30421 gtaaagttca tatactttca tagcttatac tctacacaat aattatacta tatttacttt
    30481 atatttaatc tactttaaat tattaatttt aaaaataaag tttcctctct aataaatata
    30541 aataatcata caatttaaat attaatctct attgtttaaa ttccccctag tcacaaagcg
    30601 tatattatgt gtataattaa tttactgact cattttggtc aaagaaaggg gtgctataaa
    30661 tggcattaga tggattagtt atacattcaa tagtcgatga attatcatca aaacttacag
    30721 gtggaaaaat agataagata catcaacctg aagatgatga ggtaattttt aatataagaa
    30781 ataataaaga aaattttaga ttggttttaa gtgcaagtgc ctcaaatcct agagtttatc
    30841 ttacaagtaa ttatcaaaaa gaaaaccctc taaaagcgcc tatgttttgt atgttactta
    30901 gaaaatacat acaaggtgga aatatagtag aaatttctca aataggtttt gaaagaataa
    30961 taaaaataag tgttgaatct ttagatgaat taaaagaaaa gacagttaaa aatattatga
    31021 ttgaaattat gggaagacat agcaacataa taataactca tggtgaagaa aataaaatta
    31081 tagattctat aaaaagagtc ccttttagca taagtagagt tcgtcaagtt ttacctggtc
    31141 atgattattc attaccccca gaacaaaaca aactcaaccc tcttgataat atatctaagg
    31201 atttgtttat taaaaaccta gaggaattgg aaggacctat ctttaagtct atatattcta
    31261 aatttctagg tataagccct attatagcta aagaaatttg ctacagagca ggtgtaaatc
    31321 aaaatgctat cataaaggat atatctgatg aacaatttga tgctttacac aaagtatttt
    31381 gtaatttatt caatgatata aattctaaca aatactctcc ttgcattatt atagataaaa
    31441 aggtggataa ggtagttgat ttcagttgta taaaccttac attatttagc gatttatcat
    31501 atataaataa ggatagtatg tctcgtattc tggaagattt ctatagaact aaagatatta
    31561 aagatagaat taatcaacgt tcatctgatt taaagaagag tatatctgtt aagcttgata
    31621 gactttataa taaacttaaa aaacaagaag aagaattatc agaatccgaa aatgcagaca
    31681 tttataagat aaaaggagaa ttgattactt catatattta tatggtagaa aaaggtatgg
    31741 aaagtattga agttgctaac ttctatgatg aaaattgtaa tgatgtaaca atagaattaa
    31801 ataaaaactt gacaccatct gaaaatgctc aaaaatactt taaaaagtat aacaaaatga
    31861 aacatgctaa agtcgagata tctcatcaaa tttccttaaa taaggaagaa atagattact
    31921 tagaaaacat aattttaagt attgaaaact gtgaaaattt agctgaatta caagatataa
    31981 aagaagaact tgctaaggtt ggatacatca agactcaaaa gaaaaacagt aaaaaggata
    32041 ctatcccatc tacaaaacct catgaatttg tgtcttctga tggatttaaa atattggttg
    32101 gtaaaaacaa taagcaaaat gactatctta cactaagact tgctgataat gatgacttat
    32161 ggatgcatac aaagaatatt cctggttctc atgtaataat aaagtgtgct ggtaaggaag
    32221 tccctgataa tactgtattt gaaggagcta tgctagcagc attctttagt aagtcaaaac
    32281 tttcttctca ggtgcctgta gactatacta agagaaaaaa tgtgaaaaaa cctagtggtt
    32341 ctaaaccagg tatggttatt tatgaaacta acagtactat ttatgttact cctgaagaag
    32401 aaacagtagc caagcttaag gttaaatctg aataaattct gcactaaata caaaataaat
    32461 gttagacaac tacacattat cattctaaat aaaaatagtg gccttcttat aaagtacaat
    32521 aataaggcca ctttctaatt agcttacttt taattttcta acaaaatact tttctgtttt
    32581 aataactata cttatattcc caacaaatat tttcagtctt cttatataga tgtaattaaa
    32641 aagttaacac taaaagctat tttttaattt attgatttgt tttagtaatt cattatattc
    32701 tgattcatac aattcttttt ttagattatc tttttctata gataacatag atataacagt
    32761 tgtaagtcta ttttctaata ctagaagatt ttcttttttg atttggtttt ctctactagg
    32821 agttttttta tttctgctaa ttgtatattc gtcataatta caatcaaact cccttatttt
    32881 agcatctttt atctctataa tatagttaca aatatgtgat acaaatactc tatcatgaga
    32941 cactataagc atagttttat cagtatttat cagtgccttt tccagtgctt ccatagattt
    33001 tatatctaag taatttgttg gttcatcaaa tactaaaaaa ttattatctt ccaatattat
    33061 tttacatagt gctattttta ccttttctcc accactaagt acttttactt ttttgtagac
    33121 atcatcacct ttaaacccaa ataggcttaa gtttatcctt ataaaagatt catcaaatga
    33181 actattaact ttagtattgt atagaacact cttttcctca tctaataaac tttgattttg
    33241 gtcaaaataa cctactttta ccttgttatt tatttttata ttgtcatttt tatcagccaa
    33301 tatttctttt atcagagtac tttttccaca cccattatca cctaataatg ctatcttttt
    33361 acctcttttt atcttaaagg aaacattatc taacaggagc ttattttcta actttagagt
    33421 catatctttt acctctacta gatttttgct aattatatcc ataccatctt gaatcttaat
    33481 gttcatttct ttagaaactt taggtttatt ctttgcctca agtttatcaa ttctgctttt
    33541 tatagccttc acatttgagt ccaacttttt cttattagtc tgaccaccca ttttgtgcaa
    33601 cctagcttct gaattaccca ttctttttgg tgtttttctt atgccatcac ttaaagcttt
    33661 tttatataaa agagcttttt caagcctttt tttctcttta acatattctt gatactcaaa
    33721 atcagctctt tttacttctt catctttttg cattaaataa tcagtgtaat tgcctttgta
    33781 agcttttagt tttccatctt ctagttctaa tatagttgta cataactcat ccagaaaacg
    33841 tctatcatga gatattaata ataatgctcc ctcatgcctt ttaaacatat cttccaaaac
    33901 cccaatgctt tttttatcta agtttgaagt tggctcatca gctattatga tatttttctt
    33961 attactcagc gcatctgcta tttttaactt aaccttttcc cctcctgata agtgttcttc
    34021 aaatttatct ggagcattta aaagactctt ctccttacta tgaccacatg tttcaatatt
    34081 attttcactc tgacttatat aagaatagct ttctgtaagg taagtatatc cttcatcaat
    34141 ttctatttct cccaatattg cttttaataa tgttgttttt ccttgaccat ttgaaccaac
    34201 tagacctatt ttgtcatttt ctaatatttc aagtttatct atatccaaaa ttaatttatc
    34261 tgcataatat ttctttaaat tctctacttt tactaacaac ataaaaaatc cctccttaac
    34321 acacttaaat ataaggagag attctataaa taggcataac aaataaacgt atagactata
    34381 atctactctc cttaatttaa gcattgcaaa taaagccacc aaaataggct tttcaaaaat
    34441 tacaatactt taaattataa aaaatagatt atattctcat tgtcctagtt accttgccct
    34501 ttctttcatt tatttttaat cattatatca acattaatta caaatgtcta tagaaaatta
    34561 actaatttta gttattcaat atacaactta ttctttaaat ttattcttct tcaactttaa
    34621 agtcctgatt atttaaaatt ctgtttaata caattccaac aattgcagct aaactaagac
    34681 cagttatctt tactgtactt gttataggga ttccaacaga aataccttta ttagataaat
    34741 atgttgttcc aagacctatt aataatataa ctgctatgat taatatattt attttacttt
    34801 ctacacaaga actgtccctt atagttttta atcctacata agttatcatc gaaaatagca
    34861 ttatactaat tccacccata actgaacctg gtatactttg aagaaatcca ccaaacttgc
    34921 ctacacatgc aagcaatata gcaaatattg ctgtacgtct aagtattgaa gggtcatagt
    34981 ttttagttat agcaagtact gctgtatttt ctccataagt tgtatttgct ggtcctccta
    35041 aacaccctgc aactatagtc gcaaatccat ccccaagtaa tgttctattt aatccgggat
    35101 tttctataaa gtttttacct actacagcac cattagtagt catatctcct atatgttcta
    35161 taaatacagc taatacaact ggagatataa tcacaattgc tcctaaactg aacttaggaa
    35221 ctgtaaagtt aggtattgca aataaacttg cttctgtaat agtagttata tctactaatc
    35281 ttaatatcaa acagattata tacccagaaa aaactgctat taatattcct aattgtttta
    35341 taaatcctct accaaactta ttggttaaaa ttgctattgc taaagttata aatgctatca
    35401 caaaattggc tgaagccata ctaaaagctg ttggcaataa atttaatcct ataactacta
    35461 tcatagctcc tgtaacttgt gcaggaaaat ataatttaat tttatttaca ccaactactt
    35521 ttactataat cgacattata atataaatta atcctgctac tattatacca ccttgagcat
    35581 atgctaaatc tccaccatat gcctctttag ctgctagtat aactggtata aacgcaaaac
    35641 ttgaacctaa aaaggcaggt acttttcctt ctgtacaaaa atgaaatatc aatgtaccaa
    35701 caccagcaca gaaaattgca actgacggat taaaacctgt caaaatggga actaatactg
    35761 tcgctccaaa catggctaat agatgttgta aagacattat cgtttttttc ataaaaacct
    35821 ccaaaattat tatatataaa taaatcctct gtttaattaa tatcttttaa ttaattatca
    35881 ttccctagat tctatttttt aacctctata tacttattat ataatttatc attattttac
    35941 attttacgac atcaaaaact caaataaagg agaataaaaa ctacattaag actcacatcc
    36001 ataaaattat aatatttatt ttgtgcaatc tatgtaagct ttatcctcct ccctgactat
    36061 tcaatgctaa gcttttatat aatacttaaa aataattatt ctttgattgt aactgaatct
    36121 tctccatcaa tttctaataa actgacagat ataatttcat gtctagaagt aggtacattt
    36181 tttcctacat aatctgctct tataggcaac tctctatgcc ctctgtccac tagcactgct
    36241 aattgtattg atttaggtct tcctatatct ataatagcat ctagtgaagc tctcacagtt
    36301 cttccagtgt ataaaacatc atcaactaaa actactgtct tatcatttac attaaaatct
    36361 aaataagttc ctttaactac aggttcaaca tgaatttttt ccaaatcatc tctatacaaa
    36421 gttatatcaa tatctcctgt atctactttt gttccttcta tttgttctat tttcttagaa
    36481 attctatttg caattggcac acctctagtc tttattccaa ctaaaactag attttctaca
    36541 cctttatttc tttcaattat ttcatgactt attctagtaa ttgctcttgc tattgccttt
    36601 tcatccatta attgagcttt ttctaccata tcataattat tgtcaaagat ataatatatc
    36661 tatttgacaa atctactcct ttcttttaat tttttataaa tttattttta tttaaaattt
    36721 caatttaaac aacaaaaaaa cttctcatca caagacaaga agctcttata taaaaaatat
    36781 acagtgcaat ttagcaattg tcttacgata tctctgtatc gtttaaagat aattttttaa
    36841 tctatcaaat caaattatat atttatattt caaatttttc aagctttttt aaacaaaata
    36901 ctttaatata agatatttta tttaaatttc actaaattta tttacttata taaaaacaaa
    36961 aaatgattta taagtattat acaaatggtt attttatctt tttaataact tccttaaaat
    37021 actcaggtaa ttctgaatta aattcgatat attccttagt agtaggatgt ataaatccca
    37081 acttcttagc gtgcaaagtt tgtcctttta atttaaattt agtttctttt ggaccatata
    37141 cataatcacc taaaagtggg tgatttatag acaaagcatg aactcttatt tgatgagttc
    37201 taccagtttc aagcctagct ctcatatgtg taaacttatc aaatctttct acaacttcaa
    37261 agtgagtgat tgcatttttt ccatctttta ctacagccat ttttaatcta tccttaggat
    37321 ttctccctat aggtttatca acagttattt tatcttcttt aaccacacca tagcatataa
    37381 attcatactc cctagttata gtatgttcct ttaactgttc tgctagaaaa ttatgtgcat
    37441 aattgttctt agctatcata agaagaccag aagtatcttt gtctattcta tgaactatac
    37501 caggtctaat aactccattt attgatgaca acttgtcttt acaatgatat aaaatcccgt
    37561 ttactaacgt attttcatag tttccaggag caggatggac aaccatatcc tgaggtttat
    37621 ttattattaa gacatcatca tcttcatata caatatctat tggaatatct tgaggtaaaa
    37681 cttctaatac tttaggttct ggtatctcta taacaatatt atcttcctct ttaaccagat
    37741 atctagcttt ttctacttta ttattaacct ttacttttcc atcttttata attttttgta
    37801 tataacttct tgacatatca cctaattgtt ctgctagata aatatcaagt ctaacttctt
    37861 cttcctcttc aagtactaaa aactctttta cttcatccat tatatcacct acttttactt
    37921 tcaaaaaata aaacatatat acataaaaac acagttccta caactacaaa tacatcagct
    37981 atattgaata catattccca tataattcta aagtcgaagt aatctactac aaaacctaat
    38041 cgtactctat caattagatt tcccaatgca ccagatataa ttaatataat tcccaacctt
    38101 ccaaatatat gtactttcct tgtattaaga tagtatagtc caaatactgt tgcaagtaat
    38161 gcaacaatta taaatatcca ttgattattt tgtaataatc caaatgctgc acctctattt
    38221 tcgacataag ttaaatgaaa tacattattt attattggta ttgttgatac atccaccaaa
    38281 ttattcaata cccatatttt agacagttgg tctaaaccta tgagtagaat tattattaat
    38341 atatatagca atgatttttc cccctataaa ttatttaaaa accaatttta tttgaagcac
    38401 actattatta ttatacaaaa aaaatgctct aaagagcaac tttattaaaa ttgtattttc
    38461 ttaataaagc tagaacaaaa tttaaaatag tactactttt tttatattgt agatttctac
    38521 atatttaaaa tcatatcatt atgtataaaa aatttaaaaa taccatacta atcaatattt
    38581 tttgtgtatt ttatagatta aagattaatg atgtctgtaa tttaaactta caatcaccac
    38641 taatcttcaa acaaatattt ttacttttgc acttgcttat ttttatctaa atgttgtata
    38701 agttcagata ctgttacgaa tttatatcct ctctctttta atttaggaat tactacttct
    38761 aaagcttgta tagtttgtga tttatcattt cttttagtgt tataatcatg taaaagtatt
    38821 atattaccat tttggacctt tgtaaggata gtatttataa ttgcatctac tccagggtta
    38881 gaccaatctc tagggtctaa gtttgaccaa agaattatat tcatgccttt agaaactata
    38941 atatcacaga cacttttact catggctcta tatggaggtc taaatatctt tggttctact
    39001 cctattacac tttttataat attttgcgta tctgttattt ctttttcaac actactataa
    39061 ctattttttg caacatttat atgtgtaaat gtatgattcc ctatttcatg cccctcatca
    39121 tattgtcttt ttaataattc tttgttatat tctacttttt ctccaactac aaaaaaagtt
    39181 gcttttacat catttttctt taatatatca agtacttgtg gtgtaaaatc ctcatctgga
    39241 ccatcatcaa aagtcaatgc taaaattttt tcatcctcat ttcctttttt tattattaca
    39301 tcttcataat cgtcttttat attgtcattt aaactatatg cttcttttga actatcttga
    39361 ttatgtgtat tatgactata tgtatataca cctattatag ataataaaca tatacatata
    39421 ccccatatta ttttcttcat gtcattacct cttttctaat tcattctagc aatatatttt
    39481 ataatgtcag ttttttaatc tctcaatatt ccatttttat ttaaatcagt taaatttgtt
    39541 aggtcttctg aataaaagtt agaatcttct acatcagggt tttgcattgc atcacctaat
    39601 aaaatatttt ctttctttgc acaatcatta cataaacttg tatctggcaa tatatccaat
    39661 ctttctaaat ctatttgatt atgacataaa tcacaaatcc catatgttcc attctctatt
    39721 ttagataatg ctaagtctat ttgatacaat cttccctctt catgatttat tagactgttt
    39781 tgcatatttt gcatatataa atctgttccc atgtctccta tatgattatc ataactagat
    39841 aattctcctg atgaatactt ttcacttgta tgtttagttg tatctccaaa taaagtatta
    39901 tctttcatat cctctactaa attagttaaa ttttctcttt ctttcaataa tttatcttta
    39961 tattttttag tatccatttt taacacctct aattttaata tttacatcat aaaaatagta
    40021 tgtggaaaag taaattaaac atacaataaa aataagattt gattcgaaaa atagaatgtc
    40081 tcaaaatgaa atttttagtt catttaaccc tcttttttat ataaaatcaa atatattttt
    40141 atcctttcag caataaaaaa gagacttatc tctattttga gatagcctct tttttgaaca
    40201 taatttaaat acaatttttt aatttttata gttattattt tactaacttg ccaatttatg
    40261 attattttgt tgcttaatat gctttgcttt tttaaagaat aaaagaccaa gaattattgc
    40321 tacaataaca cctattccat tagatatatt aactggtagt ctaaatcctt cttttgcttg
    40381 cattatataa ctaaatgtta ctacagtcat aaaaagtgct ggtaatgtag ttatataatg
    40441 gtttttattt gctttgacta aatatgcaga accagtccac aacattatca ttgcaagtgt
    40501 ttgatttgcc caagagaaat atctccaaat tatattaaaa tctataaatg taagagcaac
    40561 accaactgcg aataatggta aagctattaa aaatctattt ttgactgcat cttgattata
    40621 attcattgca tctgctattg tcagcctagc acttctaaat gctgtatctc cagaagttat
    40681 aggacatgcc acaactccaa gtatagctaa agctcctcca ataggcccca ttaatccaac
    40741 agatactgta tttactactg ttgctgctcc accatcatta aatatcacat taagttgagg
    40801 tatgccatgg aaatatgaca tggctgctgc tgcccaaact aaagctatta taccttctgc
    40861 aaccattgac ccataaaata ctggtcttgc ctcactctct ttctcaacac atctcgccat
    40921 aagtggtgat tgagttgcat gaaaacctga tattgctcca catgctattg ttataaataa
    40981 gtatgggaat aaaggtgttc catttggatt catattttgt aaatttatat ttggtatgtt
    41041 ataccccttt attattaatc ctccagctac acctactgcc ataattaata atgctgctcc
    41101 aaataaagga tatattttac caatcaattt atctattggt aaaacagtag ccataatata
    41161 ataaagtatt atcactccaa cccatatttg tgcgtttaca cctgtcaatg tctttaataa
    41221 tcctgctgga cctgttacaa atacaactcc aaccaatact aaaagaataa cactaaatat
    41281 tctcattgct atttttgcac catttcctag ataaaggcct accaactctg atgcagatgt
    41341 acctttgttt ttcattgata gatatcctga agcaaaatcg tgtactccac cagcaaagat
    41401 tgttccaaac acaatccata aaaatgctga tggtccaaat aatgcccctt gtatagctcc
    41461 aaatatagga cctaaccctg ctatatttag aaactgaatt aaaaagattc tcttccattt
    41521 cattggcaca taatctactc catccctaca agctatagct ggagtttgat ttttgtcatt
    41581 gattccaaaa accttttcta caaaagtacc atagacaaaa taacctacta taagtactac
    41641 tattgaacct aaaaagctta ccataaattc atccccttag tttttatata attgctttat
    41701 aaatttttat gagtttaaga aaactatttc cagttcaaat agtaatactt ttttatgcta
    41761 atataaatat taattacatt aagtgcatat ttaagtacat gaaatgactt tatatatgca
    41821 ttaacagcct aaattctttt actttgcttc tacttacagt tacttcatag tcactatttt
    41881 ttaattttaa tatgtatgta ttattaaacc atggtataat ttcttttact ttggttaagt
    41941 ttacgatata agacctatgt gttctaaaaa atgttttatc atttataaga ttttcgactt
    42001 ctgatatgcc ttcttttaat ataaactttt ctttctcagt atatataaat gtctgccttt
    42061 cattagcttc acagtaatat atatcatcaa tatctattac aactaattta tcacctttcc
    42121 aaaggctaat tttatgtgtt atttcttcta tctcctgatt tactgcttcc ttcttatatt
    42181 tgtataaatt actatttaca ttagatagtt caatatcact catctcactt ttttctaatt
    42241 tattcagcat agaaataatt ctctcttcag aatatggttt caatatataa tcaaatacat
    42301 ccaaagaaaa agcatctact gcataacttt catatgctgt tataaaaaca attttaggtc
    42361 ttgatttaaa ctgatttagt gtttttgcaa gtagcattcc atcaagatga ggtatattta
    42421 tatctaaaaa aattacatct attttatttt cctgaataaa gtctaacacc tctataccat
    42481 tggtaaattc acttactacc tctattccac ttttattttc aataaaatat ctcaattcct
    42541 ttcttgctgg aaattcatct tccactatta tagctctcat acttctactc ctttacataa
    42601 aatactatca aagtcccatt atctattctt cttatattaa gccctcttcc atacatcagt
    42661 ttaagcctta aatgtacatt ataaagccca atcttatttt ctggcatatt atcttgatat
    42721 acattattta taatttcctc acttatacct ataccattat cctctattga tattctcaca
    42781 gtattttcta atgaatacgg taactttttt atggatattt tcactacacc agctctacca
    42841 ctttctaaaa taccatgtat tattgcattt tccactaatg gttgtataat caaacttgga
    42901 atctttatat ttacatcttc tatatcataa gatacagtta gtaattctcc aaacctagct
    42961 ttttctatct ctacaaaaga tttaacttgt tcaatctctt tatttatatc aattaagtta
    43021 tcactatatt caagattata tcttagataa gttgataaat ttattattaa ctctctagcc
    43081 ttatctggac taaatcttat aaatgaagct attgtgttta gggaattaaa caaaaaatgc
    43141 ggattaattt gtctttgaag tgcttttatt tctgctttat ttctcgcttc tatatctttt
    43201 ttatctttct caatcccctc tactatagat ataacaaatc ctattccaat ctgcccgact
    43261 attaaaggta aatatattcc accgattatt tgaattgcta aagaatgtgg atatgatatt
    43321 aagtatataa gtaatatagt aaaactttca gaaatcatgc ctaccaatat tccatacatt
    43381 actctatgtt gttttggtat cctcttatat agaaatcctg ataatacacc tgctagtata
    43441 cttgataaaa gacatggaac agatgttatc cccccagtat ccataaaata tctatgaatc
    43501 cctgaaaaaa ctcctgctgt aatacctacc attggaccaa ataaaatgcc acctgatact
    43561 atagatatta ttctagtgtt tactattgac cccatataat ttataccact ataagttcca
    43621 aatatggcta aaaaagtaaa aactaatgct atacagacca agtcattaaa actatattct
    43681 tccttttgaa atatctgctt aaatattttt aactttgtta ttagaagaaa caatactaca
    43741 ataaggcttg tcttttctat taatgcgatt aaaattgatg ttttcataat ccccctttaa
    43801 aaaataaaac attacttaat ttaagtttat tattcttata ataaattaga tacataaaaa
    43861 taaattccat aattattgta tcatatcacc tatatataaa cataaattca tttaattata
    43921 taaatttaaa aaatattttt ttgatatatt attgacaatt ttcattataa gagttaaaat
    43981 taactcataa attacatatt gggtataaaa tacaatgtat gttagaagtt gtttcagaag
    44041 gctatggttt tctgattttt ttatgctcag tagtgatatg caaaaaagac aaagttgaca
    44101 tcagttaact tttaccttta ttctactttc cccaaagtaa gaataatcta acccctttga
    44161 gatgagcaaa agctcatctt tttttattat caaaagatgc tgtctcaaaa tagagataat
    44221 cctctttttc attgttgaaa tgattaaaat atattttatt tcatacaaaa aagagtgcct
    44281 catgaacaaa agttcacttt gagactctct ctctcatatc atataccttt ttaataactt
    44341 taaaatttat ttatcaagta aagatagcta ataattagta tcttctctct ataaatccaa
    44401 cttttttata aacttttcta agagtttttc tagcttgttt ttcggctttt tctgctccca
    44461 tactatatat tttttctaaa taatctttat tattaagaag gtcaacatat ttttctctta
    44521 taggtcttaa agcttctgat actacttcag ccacatcttc tttaaatatg ccataaccct
    44581 ttccttcata catgtttaca atctcttcta cactcttttt acttaaatta gaatatatat
    44641 caattagatt tttaatacct ggttgttcat cattgtattt tacaacacct aaagaatcag
    44701 taacacttct ttttatcttt ctctttatag aatctgaatc atctgctaat agtataaatg
    44761 cattttcatt tgaatcggat ttactcattt ttttagttgg ctcttgcaaa ctcatcactc
    44821 ttgctccacc ttttgggtag tatccttctg gaactacaaa agtaggtgaa aatctattat
    44881 taaatctatt tgctaaatca cgagctaatt ctaaatgttg tttttggtca tctccaactg
    44941 gaactaggtc agtttgatac aacaatatgt cagcagccat aagaactgga tatgtgaata
    45001 aacctgcatt tagatttgct tcactctttt gtgatttatc tttgaattga gtcattctac
    45061 ttaattcacc catataagtc atagtattca atacccacat aagttcaaca tgctctttga
    45121 catgtgattg tataaatata gtattttttt ctgggtctaa tccacaagct atatattgag
    45181 caagtaattc tatagtattg gctcttaaaa ctttagggtc ttgaggtact gttattgcat
    45241 gcaaatcaac tatactataa taacaatcat aattgtcttg aagttcagtc cagtttttta
    45301 tagctcctaa ataatttcct aaagtcattt tgcctgatgg ctgtgcacca cttaatataa
    45361 cttttttttc actcattttt ttgtacctcc ttatatattt atattgattt taacatattt
    45421 aaaactatgt ctttagagtt attggcagca tcatgtacaa atacatcata agtaacatca
    45481 gcacttccat ctgccttatc agacattgct cttattatta caaatggagt attatttaaa
    45541 tagcatacat gagctatagc tccaccttcc atttctccac aatatccacc aaaatcattt
    45601 actaattctt cttttaattc tttagaattt ataaatttat ctcctgtaac tactcttcct
    45661 tttaatactt tatgagtctt tacttcttcc acagaagatt tgtaagcagc atctataagt
    45721 ctttcatctg ctttaaatac agaagtcttc attcttggta taactccctt agggtctcca
    45781 aatgctgtag tgtctacatc atactgtata gcatcagttg atattactat atcattaaca
    45841 tcaagttttt cgtttaaagc tcctgcaaca cctgtattaa caatagcatc aaccttaaat
    45901 tcgcttatta gtatctgggc acaaagagca gagttaacct tacctatgcc acactttact
    45961 aacactacat tcttaccttc caaaattccc ttataaaatt ctaagctagc tttttttata
    46021 gtctctctaa tatccattaa atcaactaaa atactaactt cttcatccat tgcacctata
    46081 attcctatta cgttcatttt tcctcctaaa ttttctatat taaaaaatcc gtcctaaatc
    46141 aataggacgg atatattttc cgtggtacca cctaaattta tatacactca aaattgaata
    46201 tatatatctt aatatatgct ataacgtgca tacacgatat caaatactct gtaatcttaa
    46261 attacatttc attttactcc tcaaagaccc attcaataaa tactttttat taagttctca
    46321 gcatccttaa ttctctgtgc aaaaatatat ttatttacta tttcttatct caggatttaa
    46381 tttattaatt tctttatttt ataatattcc ttacactata aattgtcaac tatatattat
    46441 aatatccaaa agaaattttt aaaatacatt ttatttagct gttttctttt catatctatt
    46501 ccataaccat agaccagata atataactag aagacctcca ccaatcatct ctatctcata
    46561 tataacattt tcagcaaatg taggcataac tggtgctgca gataataata tacccactac
    46621 agttacaact aaaaccattt ttgctaaacc taggcaagta cttgttgatt ttgccatagt
    46681 ataaggtcta atttcatctg gtctctcttt tcttagttta atataagaag catataacaa
    46741 tacatatggg aataatgcag ttaatgctgt catagttaca agaacattgt atatagcatc
    46801 aacagaaggc attaatgtag tcaccaatag tattatagta actattattg cttgaagtat
    46861 aactgcttta gaaggtatct tgtgttcatt tacctctact aaactatctg gaaatattcc
    46921 ttttttaaca ctaccaaata acatttttat tggtgatgct atgtaaagaa ctattgctcc
    46981 taatactgaa aaagctatac caaaagctat taattgaacc aaccaagaac caattcctaa
    47041 agacgcacaa acagcagaaa gtgcatctaa tacaccttta gaagcagtaa tttggtctgg
    47101 tggtattatc attgttattg caattgaacc taatacatat aatgaagcta ctattccagc
    47161 tgatattaat attgccttag ggaagttctt ttcaggctta tctatttctg ttatgaagtt
    47221 agcagcagtc tctgctcctg ctaaaccaaa cattatagct gatatagcaa caaatgcatc
    47281 catattaagt tttggagtca ttgaagctat tgtgtatgta gaagctatat catgtttttt
    47341 caacacaaat actgctacaa atgaaaatat tataagtata gcacttggta ttgttgaacc
    47401 taatgcacct gtattagtaa atatttttgt aaaacttatt cctttagttg ctataagaga
    47461 taatgcccag aatattatta aagataatat aagtacaaac atcttattat cagcaagggc
    47521 tggatttcct attgtataag aaacatttac tgctaagaaa gttaaaaatg aagaatacca
    47581 aaatatttta gctgtccagt ttaaccaaga aactaaaaat ccatactttt ctccaaaggc
    47641 ttccttaacc caatcgtaaa gacctccatc acctttttga tatgtagaag ctaattcagc
    47701 acaaattaaa gctcctggta caaagtatat aaatgagaat aataaccaca taggtattgc
    47761 acctaatcct aaaccaaagc ttgatgctgt agatttagct atccatctta taccatataa
    47821 agctgataca ttcataaata ccaaactcca taggcctatt tttttctttt ccatcctaat
    47881 ttaatttccc ctctctatta attattttaa atttaatttc aaaattaata aaatagattc
    47941 attattatta aactgatatt tttcaaaata tttg
//
LOCUS       DAFKMX010000006        47850 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFKMX010000006 DAFKMX010000000
VERSION     DAFKMX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.006"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            25..906
                     /locus_tag="LZB08_000272"
     CDS             25..906
                     /locus_tag="LZB08_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBM7313793.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGATSPYQVTKFKNKKDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1219..1998)
                     /gene="hisJ"
                     /locus_tag="LZB08_000273"
     CDS             complement(1219..1998)
                     /gene="hisJ"
                     /locus_tag="LZB08_000273"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBM7313794.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2544..2894)
                     /locus_tag="LZB08_000274"
     CDS             complement(2544..2894)
                     /locus_tag="LZB08_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBM7313795.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2910..3326)
                     /locus_tag="LZB08_000275"
     CDS             complement(2910..3326)
                     /locus_tag="LZB08_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBM7313796.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3345..3536)
                     /locus_tag="LZB08_000276"
     CDS             complement(3345..3536)
                     /locus_tag="LZB08_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBM7313797.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3687..4502)
                     /locus_tag="LZB08_000277"
     CDS             complement(3687..4502)
                     /locus_tag="LZB08_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBM7313798.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNIEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4549..5487)
                     /locus_tag="LZB08_000278"
     CDS             complement(4549..5487)
                     /locus_tag="LZB08_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7313799.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5484..6356)
                     /locus_tag="LZB08_000279"
     CDS             complement(5484..6356)
                     /locus_tag="LZB08_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7313800.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6381..8309)
                     /locus_tag="LZB08_000280"
     CDS             complement(6381..8309)
                     /locus_tag="LZB08_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBM7313801.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGIIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8421..8798)
                     /gene="gcvH"
                     /locus_tag="LZB08_000281"
     CDS             complement(8421..8798)
                     /gene="gcvH"
                     /locus_tag="LZB08_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBM7313802.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8885..11011)
                     /gene="cdhC"
                     /locus_tag="LZB08_000282"
     CDS             complement(8885..11011)
                     /gene="cdhC"
                     /locus_tag="LZB08_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBM7313803.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11068..11874)
                     /locus_tag="LZB08_000283"
     CDS             complement(11068..11874)
                     /locus_tag="LZB08_000283"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBM7313804.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11924..13291)
                     /locus_tag="LZB08_000284"
     CDS             complement(11924..13291)
                     /locus_tag="LZB08_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBM7313805.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13322..14266)
                     /locus_tag="LZB08_000285"
     CDS             complement(13322..14266)
                     /locus_tag="LZB08_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBM7313806.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14286..15056)
                     /locus_tag="LZB08_000286"
     CDS             complement(14286..15056)
                     /locus_tag="LZB08_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7313807.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15112..16497)
                     /gene="lpdA"
                     /locus_tag="LZB08_000287"
     CDS             complement(15112..16497)
                     /gene="lpdA"
                     /locus_tag="LZB08_000287"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBM7313808.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16541..17422)
                     /locus_tag="LZB08_000288"
     CDS             complement(16541..17422)
                     /locus_tag="LZB08_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBM7313809.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKLLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17458..18132)
                     /locus_tag="LZB08_000289"
     CDS             complement(17458..18132)
                     /locus_tag="LZB08_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBM7313810.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18169..19041)
                     /locus_tag="LZB08_000290"
     CDS             complement(18169..19041)
                     /locus_tag="LZB08_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBM7313811.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19133..19765)
                     /locus_tag="LZB08_000291"
     CDS             complement(19133..19765)
                     /locus_tag="LZB08_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBM7313812.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19870..21546)
                     /locus_tag="LZB08_000292"
     CDS             complement(19870..21546)
                     /locus_tag="LZB08_000292"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBM7313813.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21795..22574)
                     /locus_tag="LZB08_000293"
     CDS             complement(21795..22574)
                     /locus_tag="LZB08_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7313814.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22884..24803)
                     /gene="cooS"
                     /locus_tag="LZB08_000294"
     CDS             complement(22884..24803)
                     /gene="cooS"
                     /locus_tag="LZB08_000294"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBM7313815.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25410..26321)
                     /locus_tag="LZB08_000295"
     CDS             complement(25410..26321)
                     /locus_tag="LZB08_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBM7313816.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26437..27015)
                     /locus_tag="LZB08_000296"
     CDS             complement(26437..27015)
                     /locus_tag="LZB08_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBM7313817.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            27241..28164
                     /locus_tag="LZB08_000297"
     CDS             27241..28164
                     /locus_tag="LZB08_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7313818.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(28336..29478)
                     /locus_tag="LZB08_000298"
     CDS             complement(28336..29478)
                     /locus_tag="LZB08_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBM7313819.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29496..31250)
                     /locus_tag="LZB08_000299"
     CDS             complement(29496..31250)
                     /locus_tag="LZB08_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBM7313820.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31386..31592
                     /locus_tag="LZB08_000300"
     CDS             31386..31592
                     /locus_tag="LZB08_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313821.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31597..32520)
                     /locus_tag="LZB08_000301"
     CDS             complement(31597..32520)
                     /locus_tag="LZB08_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313822.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDHDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIADPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(32774..34474)
                     /locus_tag="LZB08_000302"
     CDS             complement(32774..34474)
                     /locus_tag="LZB08_000302"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBM7313823.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(34884..35297)
                     /locus_tag="LZB08_000303"
     CDS             complement(34884..35297)
                     /locus_tag="LZB08_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBM7313824.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(35363..37741)
                     /locus_tag="LZB08_000304"
     CDS             complement(35363..37741)
                     /locus_tag="LZB08_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBM7313825.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     ISQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            38060..39511
                     /locus_tag="LZB08_000305"
     CDS             38060..39511
                     /locus_tag="LZB08_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBM7313826.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(39715..42228)
                     /locus_tag="LZB08_000306"
     CDS             complement(39715..42228)
                     /locus_tag="LZB08_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBM7313827.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALTKGVNYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(42451..43272)
                     /locus_tag="LZB08_000307"
     CDS             complement(42451..43272)
                     /locus_tag="LZB08_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7313828.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(43790..44266)
                     /locus_tag="LZB08_000308"
     CDS             complement(43790..44266)
                     /locus_tag="LZB08_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBM7313829.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(44300..44878)
                     /locus_tag="LZB08_000309"
     CDS             complement(44300..44878)
                     /locus_tag="LZB08_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBM7313830.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(44982..47582)
                     /locus_tag="LZB08_000310"
     CDS             complement(44982..47582)
                     /locus_tag="LZB08_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBM7313831.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14640 a         9169 c         5377 g        18664 t
ORIGIN      
        1 taaatttaaa aaggagaact aaatatgaaa aaaagattaa taataatgat attgtctgtt
       61 gtcttagtac tatcaagtat acttacgata tttgcatatt caaatattaa atataataat
      121 aataaattga tatattctaa tatgatagat aaaaaaactc aaaatagtgt aaaggaaatc
      181 ctaaaagaaa ataaaattaa tgaaaaagac atagatacat ttattaaagc agtcaacaat
      241 tataataaat tgcaagttaa aatacttcaa aataatataa atatatctaa atctggatat
      301 tcttcaatca atgcaaaaca agttccatat aacctagaaa aattgcaaga taattgggtg
      361 aaaaaattcc ctgattatat ggatgtaaat tgtagaataa ctgcttttag actttttaaa
      421 gattttataa attcaaataa gaaatttact ggagactcta ttgatttgaa tgtcgacttg
      481 gacactataa tgaataataa agatgctaaa tttagcacca aagatgtaga aaaatttatc
      541 aacttctttt ctgcaatacc agctaaagac acagatgata caatcaaaat tgcagaacaa
      601 ataaaaaatg aatggaaaaa aagaaaaata tcttttaaaa ataataaaaa tatatctatt
      661 ataaatggat ttcttcgtta tccagaaact aaaaacgtat ttataggtca tacaggcata
      721 tgtattaaaa ctaaaaatgg cattctattt ttagaaaaat acggtgctac atcaccttat
      781 caagttacta aatttaaaaa taaaaaagat gtaaaaaatt atatgtttaa tagattgaag
      841 atgtcagaag gtgaaattga attacctgac cctataataa tggaaaatga taaacttatg
      901 aaataataac tttaaacgca aaatagtatt tataattact aagtcaatat aatagagaag
      961 gtctatagat ttgtgtaaat acaagcttat ctacaatctt atataagaat gtttatataa
     1021 ataagataga ttaattctaa gtcctattat tctattgaat tttattatta ttttatttag
     1081 attctattgt tattttactt agaacttatt attattttat ttaagttcta ttactatttt
     1141 caatcatata aatttacaaa agactgtaca taaatttatt ttatacaaac ttatctacag
     1201 tcttttgtgt gaatatatct aaataatatc gtatttattt atttcctctg cacagtgaga
     1261 atcagaacct aaaactaatg gaatatcgta tttttttacc aactctaata agtatccatc
     1321 tatataaggc tctttgcagt actcttttct atttccagaa acattataat caagttcata
     1381 acctttttct ttaattaact ttattaactc ttcaagaatt gtatttcctt tataatcgta
     1441 tggaaatacc tgattaaact ttctaactaa atttaaatga cctattcttt ttggtttatg
     1501 tatacccaaa tctgaattta cagcttttat caatgtttca taatacttat tataaacagc
     1561 ttcaatactt cctaatttgt cgattaaatt ttgaaactcc tctacactgt aatctacaca
     1621 ataataatca tcatcaatct taattatatg tactgataaa agagaatcct ctaattcctt
     1681 tccatattta tttaaattct cctttatccc ttcttcgtat ccttctataa aatccacttc
     1741 cgacccaaca tttatcttga ttttaccctc atacttttct ttcaacttct tgacatcatt
     1801 aaaatacttt ggtaaatctt caatactcat acaactatca ttttcaggag cagggtctct
     1861 aaatccattt gcatacggaa aatgttctgt aaatgtcatt tctgttattc caacatccat
     1921 tgctctttgt acatattttt caaaatcatc cttacttcca tgaggacaat aaggtgaatg
     1981 tatatgacca tctttcataa ataattcact ccttttttaa agaaaaaatc taatataaat
     2041 gcataaattt tttaacttca aaataatatt tttaatcatg ctaattttta ttatatctaa
     2101 tattagagta attttaaact gccttttaag tatcttatca ttagtactat tttcaactta
     2161 acattaatat tattttttaa cttaacatta atactatctt taaatattaa tactcaatat
     2221 ataattactt aacataaaat aactcaaatt gcttgatgag gcttttgcaa tatttaaaga
     2281 atatataatt atttaacatt aaataatcca atcttgtaat aaaatttact cattaaagta
     2341 tattacagaa aatacctcaa aataaacttt ttagttcatg agatactctt ttttacatga
     2401 aattagatat atttttatca tttaaacaat gaaagggagg cttatatcca tctttagaca
     2461 accccttaga taaaattttt aataattaaa catcacaaaa tataaaaatc gcttatatca
     2521 aacgtataag cgattaatta tatttacata tgttttttag cctctaacaa cattttatta
     2581 gcttcttcca tatactctat agccttttca atactctttg ctgtttcttc ttttccaata
     2641 gctctagcct tttcaaccca ctctctaaat ccttcttcat gagtttcatt gtgatttatc
     2701 caatgtacta aaagtatttt taaagttttt tcatccttag actctgatga ttcctcatga
     2761 ctatgttcgt ggtcgtggtc gtgagcatgg tcatgtggat gttcatgaca tatatctcca
     2821 tgagtatgtt cgtggcagtg gtcatggtca tgatgtccat gatgatatct tacaatctct
     2881 ttatcaaaaa tcattagtta ttccccctgt tattttatat taaactcttt ttttataagg
     2941 tttacagaat cttttacaag tagtgctaat ggtttacctt ctaatccaac taactccaca
     3001 ttttctggca taataggtaa aagtatctta agtgcctcac tgtctgcaat agcttcactt
     3061 atattacaag taacttctcc catcatagaa ttaggtatta ttacagaaat aggtgcaact
     3121 attatatttg cttttttagc tgatactact atagcatttt cccctgtagc tcctttatta
     3181 gcatgagctt tcatcataga actagttgct atagaattag tccctagtgc ataaatttct
     3241 acatatgtag gtaactcttc tctcaaagaa gaaactatct gggctccaat acctccaccc
     3301 attccatcta tcactgctat tatcatatga aattccccta ttaattaatt ttcttgtatt
     3361 attatcttat ggtccatcaa tcttatttct ttaattatcc catctattat cttttgctct
     3421 cctaataaat ccgttaaata aatctttcca tcacaagggt ctatattaac cacattttcc
     3481 ataacctttt ctaactcatt attactttta caaatgaaag ctgatgattc acacatataa
     3541 atctcctcct aatatgattt ttattaaaaa tcaaaaaaag attcccttag gtatttcaat
     3601 acaccaaaga aaccttcatg atttcagttt ttgtatatta ttatacctta agtatatcac
     3661 tttaatttat gtaaagtcaa ttaaagttaa cgctttttat tatactcttc attcttgtat
     3721 ttttcatata gttctttaac taattgcttc tcataatcag tcaaatcgcc ctccacaaac
     3781 tcaatattaa attgttcagc taatcccttt actatgtttt tctgtagaat atctatgtct
     3841 atttttctgc caatttcatt ttctattcca cttgcttttt tagctgtaaa tttcatagct
     3901 ctttctttca attctggtgt cttagttttt attaatttaa acaatttttc tacatcaaaa
     3961 tccagtatta ttgaaccatg ttgtaataaa acaccatctt ttctactttg agcacttcca
     4021 attaccttct tattattaat agtaacttca tatgatgcat gtgcattaaa acatgcagct
     4081 gataaatttt ccctacttat tctctcgcct ctatttaaat tatctgtttc tataccactt
     4141 agattcaacc ctttaactag accttcactt atatatctat aagataggtt gatgctctta
     4201 tcgattaaag gattatcttc tcctataatt atactatagg ttagctcatt atcatgtaat
     4261 acagctcttc cacctgtaat tcttctagta tagtctatat tcatacatct acacttatct
     4321 aaatctatct catcttccaa tttctgaaaa tatcctatac tcaaacaagc tggttcccat
     4381 gtataaaatc taagagttgg cttattatgt cctttcttat atgcagtaaa tatagcttcg
     4441 tcaattgcca tattcattgc tccttcatat gatttattat gtataactct ccactgattc
     4501 atgattatta cgcccccatt tttagtataa atatatccta gaaatcctct atagatttat
     4561 ttctcttatt gctaaatcta tctcactttg atttaaaggt cttgaatagt tgtatatagt
     4621 acttcctggt ctttcattat agtatggtct attacaatcc ttacaacctc ttatttcaaa
     4681 aggatagcct ttgtttatgt cttttaaaat atcattgtca agctttatac tatttaagta
     4741 tccattttta aacttaaaat actcttttgg ataaccttta tttatcatat aagacattaa
     4801 ctgtaccctt ctataacttt ctatgcttgg ttgacttatc ttttccattt tagttcctct
     4861 aactggagta aatgcaaata aactaatcgt tacatcattt tcctttaaat aagtatataa
     4921 attgtatata tcttcatgac tttcacccat accaactatt atatgtgtac ttattttatt
     4981 gggatatgat tttgacattt cagttataaa ttttaacttt tcatcatagt tatttccttt
     5041 tattttttca tatagttctt tatttgctgc atctattgct attcctattt tatcaactcc
     5101 tgctgaaaaa aacttcttta tctgttcatc attttctaat tttgcagata gtgaaattgg
     5161 catgtctatc ttaccactta tatagtttat aaagtctaaa acagagttat gtgcttcttc
     5221 acttgccatt gattgaatac atattctctt tatatttttt cctttataag cttttaaagc
     5281 atctaatatt tcttcttttg aaaactctgg ccaaattact cttgataatt tatcttttct
     5341 agtactactt tctattgatt gagaacaaaa actgcattta tttatgcatt tttctccaat
     5401 cattatatat gctgtggtag gaggtatatc gctttttttg tttaatatcc caagttctat
     5461 tgctgtccca actgatagtc taatcataaa acgcaacact ccttacactc atttattgtc
     5521 atattcattt ctatagcctt attctttgca gaccttgatg gtaatactat tctattgact
     5581 ccacacatta tagataattg gtctagctct tttctataca ctcctcttgg tctcatacaa
     5641 cctagattaa tttcagtatc tggtaaatta attctagctt cacataaaat atctgctacc
     5701 ccttctaact caggaggttc tacattttca tactcagtcc cttttgttgg aataaggact
     5761 ataaatgtaa gcttattagg aggatttttt tgtaggtatt ctataatttc atattcaccc
     5821 tttatttgtc ctccttttaa accaatacat atatgtggtg ctacttctgt atgttcttgt
     5881 attgtattat agacttcaat ataatcttct ttactctttt tcatcttata aacttctctt
     5941 attgtctcat catcaaacac taaatcaaaa gaaactatat ctaaatactt acacaactct
     6001 acaatgctat ctttatccat aagacctaaa tgagcattca atctatatcc ttgctctttt
     6061 aagtttttaa tggtattaat atgagtattt atagggacat ctccctcata actacaaccg
     6121 ccgctcaata gaaagctagt tatattcctt gattgtactt tttcctcata ttcttctatt
     6181 ggcaccatat tgtttaagta gtgaccgttg caatgggcac atttcaaact acattgattg
     6241 cttgtagtac taagagctag tgtctggtta ggataagcaa actctatttt atttccaaaa
     6301 ttctttaatt taatatcaaa tgctattttc attttttcag atagattctt attcatagtt
     6361 tattccttct taagtacatt ttactcttct agtaatttct ttgctgttgg gaactgctct
     6421 atattcgtat gtggtaagaa acatgcagaa ataaattcat ccatataact tggatataca
     6481 ctaagttcaa cataagttat ttgattacct atttcttcaa gtttattttt tgcatcttta
     6541 cttatgagag ctaagtaaga accaacaagt gaactattcc ctatataagt aaatttttct
     6601 ctatcaatat caggaagtaa ccctataatt attgagtttt ctatgtctag gttgtttcct
     6661 ataccacctg ctatataaac cctatctaat atactaaaat ccatacctaa actatctaca
     6721 agagtgtatg cgcctgaata tatagctcct ttagctctga tgaaattatc tatatcaact
     6781 tcattgacta ctatatcatt ttctaaatca aattcttctt taaatgctaa tacatactct
     6841 cctatctcat gctcattaaa tctaactctc ttattatcta aatccctgta tattttacct
     6901 cttctatcta ttataccttt ggtaatcatt tgacagatta aatcaattat accagaacca
     6961 catataccaa ctggtgcaca ttcatcaata atctttaatg ttggctctaa agtttcttta
     7021 tcaataatta ctttctctat tgctcctgct gatgctctca ttccacagct tatccctcca
     7081 ccttcaaaag caggtcctgc tgaacatgca cagctcatca tataatcttt atttccaaat
     7141 acaatctctc cattagttcc taaatctata aataaaacat tttcttcact tgaccatatc
     7201 cctgctgata atacacctgc tgttatatct cctcctacat aacttgcaac agatggagct
     7261 aagtacacat atgcactgtc atttacaaaa agaccaacat tttcacccat aagttttgga
     7321 gattttaaaa atggtggtat gtaaggttct tgtcttaaga agtctgtata cacacccaag
     7381 aataaacttg tcatagttgt attcccggca gccactaatg taactacatc ttccttatct
     7441 attccatttc tttcatatat tgtttttaat agtggattta tagtttcctc aactatagct
     7501 ttatgaagtg tctctaatcc atttttctta gttgaatata taattctatt tataacatct
     7561 gctccatatt ttatctgtgc atttccagaa gatgctttat ctactacctc ttttgagtat
     7621 aggtctacta aacaaacaac tactgaagtt gtaccaatat ctattgcaac tccgtataag
     7681 gaattttctt tatttccttg ttctatattt atgattgtaa gtttcttttg tttttgaaca
     7741 tatgttattg taaccttaaa atcactcttt ctaaatacag ttggcatttt tctaagtata
     7801 tccaatctaa aatctatttc attatatcct aggttatttc taacatatct ttccaatcta
     7861 tctacatcac ttatattatc atctaaattt ggttcttcca tttctatgta tttctttttt
     7921 atatttgttt taaattgaag gttatgttct tcaattattt ttttagctct ttcaaaaatt
     7981 tccctatcct cttttttatt actaccttct atcttcatac catgcataga ggatgatact
     8041 ttagaaggaa cttcaatttc tatatctgat attacctttg tacaacaagc tagtatataa
     8101 ccttgctccc actcatcatc tgttatatgt cttgtttttt ctgtatcaac ttttccattc
     8161 aataatttta ctttacactt cccacaagac acgtttccat tacaaggagc atctatgaat
     8221 atatctgcat ttcttgcaac ctctagtaat atatccccct cattacaata aacctcttta
     8281 ttgtttggtg taaaacttac ttttatcatg ttatccctcc caatttgttc tatatatgat
     8341 aaaaggaaag tatagctaaa tttgcgtgca ctcaaatttt actatacttt ccccaattat
     8401 aataatatct taatctttaa ttattctaat ccagcctcgt attctttatc acttaataac
     8461 gtatcaagtt cagccacatc agctagtttt acttttaata tccagttttc atatgggtct
     8521 tcatttatac attctggctc atcttctaat ttttcattaa cttccaatac ctcaccatta
     8581 acaggagata ttaaatcaga agctacttta gaagactcaa ctactccaaa atctactccc
     8641 ttagttacag tatcttctac ttctggtgtt tcaacaaaca atatttctcc taattgatct
     8701 tgagcatagt ctgttatacc tatatataca acatctccat caattacttt tacccactca
     8761 tgatctttag agtattttaa ttctggtaat aatttcattt cgatagaccc ccaatattat
     8821 attattgatt aaattactta aatcatatta atattatatc atattatttt aaattttcca
     8881 tcaattacat tacactttcc atagctaatg ctggatgacc tttttcttct aagaaagcta
     8941 atactccttc agaatcgtca gctatagttt catcacaaac catatcacag aagttttcta
     9001 ttccagtcat ttctttaact gtagcattta atttatcagc tacataatct tttaattctt
     9061 ttggcatcca aactattctt tctggtccac cttctgcata agcgaatttt ttagaagata
     9121 taaaatgtct tccgtgtccc ataaatcctg gagtttgaac tcctccacct gtcatagaag
     9181 caagttctcc gaaagtcata ccaactggag taacacttgc aaattctctg ttaactacaa
     9241 caaatccatt tgcttctggc attataccac agatacattc gaagcatcca caagaagtca
     9301 ttgggtcttc tagtatagag tataatgtaa cagattcaac tgcaccttga gatatttggt
     9361 ttacagtttc atttactgaa ttccatacac ctgttctatc atctaagcac tcgccttttt
     9421 ctattggttg acaaggacct gttgggtcta attctttagt agccttagca tctaaccagc
     9481 taactgctcc acaaagacca agtctttcag gagtaacaac acaaacgtga gctggtgcaa
     9541 atgattgaca taagttacaa gaatagaaag tgtcaacact ttcatcaact aatgaagcta
     9601 atctttcatc tctagcattg tacttagcta ttgcttcacc ttttaattca gaaacttttt
     9661 cagcatctgt aattatagtt atttcacact tatcaactac tgattcaaat tcatctaaca
     9721 tcttagcata taaaacttct cctatatgct caagtctaaa tcctttatca aatgcatctt
     9781 tagagattct tacccaagcc atatctcttt gtccaacgtg cattacacct tctatatagt
     9841 ttaagaagta gtggaaacgt ctttcaagaa ctggctcgaa atcttcttgc atatttttac
     9901 cagctatttt aactattaca gcaagtggta atcttaatac accatctgca tcaacttcat
     9961 ctatatttgg tcctattatt tctatcttat ggtcttcaac ttctgttgct tctttcttaa
    10021 caacaagctc ccaagcttca gttctgtttc caccaaactc agcaaacata tcgctctttc
    10081 taattctctc accttcaaat gctgctgcaa atgaaactgg gataggtatt tcagtaactt
    10141 ttatttttat tcctctagct tctaatgaag tagctactat cttatcgtaa tctttttgag
    10201 ttaataggtt cattggaact tcaagaactg tttggtcagt tataactggg aatcctaaag
    10261 ctattgctcc agcacctgca gatacaacta attcacttaa tggtccaaat gcattaacaa
    10321 atgcaggaac tctattagct gtgtactcta ataatccgtt taaatcacca ggagttaatc
    10381 caccgaatat taatgctgct cttactgcaa cagatacaac gtgtataaca gaagttacat
    10441 catatcctag aggtataact cttagctcaa gacccatttt tactccagct tctatagctt
    10501 ggtctataac tttaccaact aagaaagtta gtaaaccttt agattggtaa tcttttataa
    10561 cttttgctgc tgattctgca tctggacact caccaagaac aactgcaaca ccaggtatat
    10621 ctcctgtaac taatggtaca ccaagtgatc tgattatagg gtcagttata tgacctgcac
    10681 atggctcact atatggagca tccatagttg aatattttaa tgcttcaatt acttctgctg
    10741 ctaaagctgt agctaaacca gcattaaaag catgttctag taaatgagtt ctatttatta
    10801 aagattttac tacttctaaa gcaccttcta agtcccctaa agtgttcatc ttttgtcctg
    10861 ttgcagcata tatacaaggt aatgaatatg ctgtatcagg gaaagcaact ttatgctctt
    10921 ttccattctt ttctatagct tcagctaaca tagcttgagc cgcacctaaa gcttgttctg
    10981 accctgtaaa gattatatta tatagattca ttctatttcc ccctttaata tttacaccta
    11041 ttttttatta actataccct aaaaacccta gaaagcaaat ccaccttcat ttaactctaa
    11101 gaaagagtct gcatataaag atgccccgtt gattatgtca caagatttta cagttttcat
    11161 taattctggg tcacatggat tcattatagc tgaactaaat ccattagcca ttgccattgc
    11221 taagaatgca ctatctaaaa caggtctaac atgcttagga catccattag atacgttaga
    11281 taatcctcct gtagttaaaa gtcccatctc agtcatcatt tttatagctt ctaaaacttc
    11341 tacttgtttt tcttgcatac cttttataac taagcataat gggtcaaata gtatatcagt
    11401 tggctccatt cctaacatta agcctttttc taatatttct tggcagtaag ccatacgctc
    11461 atcattatct cttgggatac cttcttttgc acataaacct ataaccattg cttcgtattc
    11521 agctgctata tctattaaat caaatcttga gccagcatca gcagagttta ttattggttt
    11581 tgcattagtt ctatcataaa ctttaagtcc agcttcgata gcttttttat tagctgtatc
    11641 taatgctata ggaacattgt tgaactcaga ttgaagtaat ttgattcccc atgtcattat
    11701 ttcttcgcca tctctttcgg caggtcctat attaaaatct atatagtgtg ctcctgcttc
    11761 taattgctct tttgctcttt ttaagattgg agctgggtct ctttctgcta atgcttttct
    11821 tattgaaggt gatatacaat gtattctctc acctataatc ataaattttt ccatttataa
    11881 tgtccccctt taaatttaat attaatagtt agttaatatt ttattagttt gtttggcatg
    11941 ctttttcttg gtatcctttt aagaacttag gtaattccat agattcctct ggtcctataa
    12001 ctacattcca tcctggtaag ttatcttcta tatcaccttt aagtactgca acttttcctg
    12061 gaatgataag gtctttacaa ttagttactt cttctacctt actttcttta atgaaagcag
    12121 agattgagtt tccaccaaat ttaccagcag cccaagatgt aagaactgaa tatccacctg
    12181 catctggtat tactaaccat actggaactt ttgatctttc tatatcacca gcaactatga
    12241 agtaagttaa tgcaaagtca acagtaacta atactggaga attttcatct gggttgttga
    12301 ttggataaat ctttggttca actctcattg gtctttgtgg gtcagtgtat atgttttgtc
    12361 ttagtgcaaa taatggtaat gccttagcat aactaatatc atctattact attatagaac
    12421 cgtattttat agtgaatatt gatgataaag caacttccat catagggttt gaattagata
    12481 atctatttgc aaatactatt gaaggatatc caaatgttct atcttgctct tttaaagctg
    12541 ttcttctaac ttgtatagca ttagtgtaag tatcttttat gttttctcct gttacatcta
    12601 atactaattc tttatatcca gcagcttgaa ttaattcaac tgtttcatat aattcttcta
    12661 agctaccagc ttttacacct aatggtaaac ttgctccttt aactacttct atcatatctt
    12721 tgtagttctc tttagttgct ccatatacca ttggtctagc atcttttaat acttcaacag
    12781 cttctttaac tacttgtgca tcatcacaag ctaaaatata agctacttct aatccactgc
    12841 cttttatttt atctattaat tttaaatacg catctttatc tccacaatac tctaaaacag
    12901 ccatttcaac tttcatttgt tcaccaattc ttacatagtc aacttttttc atgttagcta
    12961 ttttagcatc tactgcttca tcactcatac aagtacagaa tgaaactgca tatctatttc
    13021 tacttactaa tgttttttca tgtctaaata atactgtttc tccacctagt tcatactcag
    13081 atgcaccagc accaactttt aaagctttca ttaaaggtgc agtagcctct gataattttg
    13141 ctatagcgtc atcagacata tgtgggcatt tacctacttc tacagctcct gaagcaacct
    13201 tcatagagaa agccatacaa gttgggaatc cacaatcctt acagtttttc tttggtgtta
    13261 atttaaatat atctaaagct tttaatgcca ttattttttc cccctaacct taacaattta
    13321 attaagctaa tgcgttaacc aattctttta tagtttctat tgattttgga tgacgaagta
    13381 taactgcgtt tgaaccacct actaaaacac ttgctgcagt tgaaacttcc attgctatag
    13441 ttctttcttc tggacaaccc caatctggct catcttctat tggagctata gcttctttaa
    13501 catgacctac ttcaaaagct actggtgtta tgataggcat ttgtaatgtc ttatcatttt
    13561 gaccaaatgc tgcaagtcta attctatcca tagtagatgc aacgtactca tatccataac
    13621 caactgctga acatccaaca ttcataacta tattttcacc tttaacgcct agttgagtta
    13681 acaatacgtt taattgttta gctaggttta tatcaacaga agattcagct cctactttat
    13741 gtgcataagc cataccagct gatgctccta ctcctttgta gttatcttct actgctgaca
    13801 taaataagca gttatgtcca tccaatgttt gagctaattt ttcaaataat ttaccatctt
    13861 tttcatgatt tcctgaacca gctacaacta aaggtaattt tattgcttca attactgcct
    13921 tagctacatc agcacattca tcaactgact tatctaatcc atttgggtca gaaccatcaa
    13981 attttaaaca aataaaatct gcttgtgtat tagcttcaac atatttagcc cactctactg
    14041 gacaattagc tacatctttg tataattcct tatatgaatc agtccagctt tcaggataaa
    14101 cgtctgatat ttgtatccct atttttggag aattacctac ttctccatca aaactataaa
    14161 aaggtaatac attttcccca cctaatttaa ttgctttttc ccctattcct acttcaactt
    14221 ctgatatttt tccagaatat ttttgagtag acattttaaa tgccatattt ttttccccct
    14281 taagcctatt ttaattctaa tttagcaaaa atttctttca tagccacttt agattttgaa
    14341 tcttcaggta aattaactaa aggaatacct gtagaatcat attcatatat caattcatcc
    14401 ataggtacaa caccgattag ctctaggtta tgcttttcaa tttcttcttt aacaccatca
    14461 ttcataaccc catttggaac tttatttaca atcaaaagta ttcttccaac acttaacttt
    14521 agttcctcag ctaaatctct tattctagca acagcttgta tgctacgtct tgagcaatca
    14581 ctaaccaata aaagtgtatc aacatgtcta gtgacttttc tacttagatg ttccatacca
    14641 gcttcgttgt ccatgactaa gtatttgtaa tgaccagata ttttgtttac ttgttctcta
    14701 agtatcccat ttacaaaaca gtaacatcct tcaccttctg acctacccat tactaataag
    14761 tcatatcctt cgccttcttc gattatagaa tttaatctaa attgaagata ttgtgccttt
    14821 gtcataccac cagggaatgc attgcctaac ttttctcttt gatttacttc ttctcttatt
    14881 tctcctattg ttgcttcaac ttcgatacct aatacttcat ttatgttagc attggcatca
    14941 gcatctacaa ctaatactgg tccctttttg tccttaacca aataatcaat caaaagccct
    15001 gtgagacttg ttttaccagt tccacctttt cctgcaacag ctatattgta tcccatttta
    15061 agacttcccc cttaaatcta gccacagagc cttatgtact ctgtctgtct attataattt
    15121 aggtacagaa tgaacacatt ctccatgtac atcatgaagt gcttccatta gcccttcaga
    15181 taaacttgga tgtggatgta ttgcatcccc tacttgttct actgttaatc ctagatgaac
    15241 agcaagtgac aactctgtca ataaatctgt agcatgaggt cctactactg ctgcacctat
    15301 tatcttatca gtttctttgt cagctataac ttttacaaat ccttggaaat gtcctatagc
    15361 ttgtgctttt ccaagacctc taaaatcaaa ttgacctaca ttgtattcta caccttcagc
    15421 ttcaagttgc ttttcagtct taccaacacc agcaacttca ggttcagtat aaacacatct
    15481 tggaattgct ctataatcta ctaccttagt cttacctaaa gcattttcaa ctgctacgat
    15541 accttcttta gaagcaacat gtgctaggaa aggagtatct attatatcgc ctattgcgta
    15601 tataccttct acattagttt caagatgttc attaactact acttttcctc tttccatttc
    15661 aattcctata tcttctactc cagaattgtc aaggttaggt cttctaccaa cacatactaa
    15721 tgcatattgt gcttctataa cctttccatt agaaagagtt gcaacagcct taccatcaac
    15781 tacttcacaa gtttgtactc caatacctgt aataacctta attttatctt tcttaaattg
    15841 tctaagtagt tgtttagcta catctttgtc ttcatttaat agtatttggt caaccatctc
    15901 aactatagtt acttctgttc ctaaggctct aaagaactgc cctatctcac atcctataac
    15961 tccaccacct actattaaca tagactcagg tatctcttca agtccaagaa cttcatcact
    16021 agttattaca acttttccat catatgggaa cattcttggt acaactggaa cagaaccatt
    16081 tgctagtatt atcttatctg cttttatagt ttctactgtt ccatcatctt tagttacttc
    16141 tatagtgttt ttatcaatta attttccaaa accattaact aagtttactc ctcttttttc
    16201 aaataagaac tctataccac ttattaattg gtttacaact ttattttttc tttccattat
    16261 ggcagtgaag tttggcttaa ctgtaccatc tatttctata ccaaaatcct ttgcttcttt
    16321 tacagtattc aatactcctg aagaagcaag aagtgcttta gttggtatac atcctgcatt
    16381 taagcaagtt cctccaactc ttcttttttc aacaactgtt acatctgcac caagcataga
    16441 agcttttata gctgctacat atcctcctgg tccaccacct actactacta tcttcatctt
    16501 taattccccc aaacctaata taatctacaa tcttgcaata ctataaacct gcggcatcca
    16561 gtatagcagg tatattctct tctgccccaa ttgccattat atgaacacca tcacataaat
    16621 cttcttcttt taattgtttg ataagttccc ctgccatttt tattccttca cttacccagt
    16681 tttcttttcc agctgctctt aatctttcga tttgttcatc tggaacaaat attcctggta
    16741 catttgcagt cataaattta gccattcctg gtgattttaa aggtactatt cctgctaata
    16801 ccttgcaatc catatccctt gttaattctc taaactttct cattgtattt atgtcataaa
    16861 gtgcttgtgt ttggaaaaat ttagcccctg cttttatttt ctttttcatt tttaataatt
    16921 gaacttctat tggagaatat tctggagtta ctgatgctcc tagataaaaa tctggagaac
    16981 cttttaactt attaccagcc atgtccgtac ctgccattaa agtctcagca gtttgaagta
    17041 tccccacaga atctaaatca aacactccct tagcttgtgg atggtctcct acgctagtat
    17101 ggtctcctgt tagtgccaac agattgttta tcccaaatac acctgctgac aacatttctc
    17161 cttgtatagc tattctattt ctatctctcc ctgttatttg aataactggc tctaatccag
    17221 catcttttaa taatttacat gtagcaagag aagttgctct cataactgcc gattgaaagt
    17281 ctgttacatt tgctgcatga actcttccaa ctaacagctt tgcacattct attaaatgtg
    17341 aaaggtcagt cccttttgga ggagccattt cagtagtaac tgcaaatttt ccactttcta
    17401 atgtttctct taataagctc atctctaatc ccccttttaa ccttctaaaa aaaactttta
    17461 tgaatttgct gctgcttctt tctttttctt tgcacttaaa cttcttggat tattttgctt
    17521 agaatagtct tttggtggtc ttatttctgc taagttatct aattgtccta tagcttctag
    17581 tctttcatat atctttatcc aagcacaatc attctctgag tttacttcac acttaccatt
    17641 tttagctcca ccacaagccc cattcataag acctttagca cacatagtta ctggacatat
    17701 cccacctgtc caaccaagtt cacagtctcc acaagcctta caggcttctt catattctcc
    17761 aactctttgt acttccccta taaacaaagt attgttggct ggataaactg gcttatcttt
    17821 taaattctta actattgtct gtgtaccatc tccacatgct aaagataaaa ctgcatcagc
    17881 ttccttagtc tcttctttta gagcctttaa atccttttta gactttaaaa ggttacaagc
    17941 agggtcaagc attacatatc ctaagatttt tttgccttca ccttcaagag tttccttcat
    18001 ttttaaaact tcttcttccc caccactttt acaagttgct gcacattgat tacaaccaac
    18061 taaaaccagc ttgtcaaaat cttttaagta ccctagcact tcttctaatg gtttattttc
    18121 agaaattatc atatctactt cccccctaaa ccttccacat atatatatct atttattgtt
    18181 ttgtaattta caagccttaa ctacatgttt tgcaagtata gatgttgtaa ctgaaccaac
    18241 ccccgctgga actggagtta tcattgatac tttgtctaaa actgcatttg tatcaacatc
    18301 tccacataaa tttccttctt catcaacatt tataccaacg tctataacta ccgctccatc
    18361 tttaacaaaa ctttcatcaa ccatcttagc tctaccaata gctgctatta gcacgtctgc
    18421 ctctgctgct acacctgata gatttttagt ttttgaatga catattgtaa cagttgcatg
    18481 ttcacttaat aaaagcattg atactggctt tccaactacc attgatcttc caagaactgt
    18541 taccttactt ccttttaaat caacattata gtgttttaat atctcaacta ctgctgtagg
    18601 tgtacatggt ggaaatccac ttttatcccc ttccattacc ttagcagagt ttattggact
    18661 aaaacaatct acgtcttttt ccggtgcaat aacatatttt ataacttctt catttaagtg
    18721 ttttggaaga ggtctaaaac ataatatacc attaacattt ttgtcatcat taactctctt
    18781 taatacatca atatattcct cttgtgttat atcctcagct aactctaaca cttctgttgt
    18841 tatacctatg ttttgacatc tctttagtgc tcctctttca taagataaat catcacttct
    18901 tgccccaact ctaactattg ttagtttagg atttatacct tctttaacta gtaaatcgac
    18961 ttcttttatt aattcttcac taatcttatc agctacaggt tttcctttta taatttgtcc
    19021 tttagttgac attccttcca tacattcaca actcccctac aagcaaactt aaattaattt
    19081 attctatata ccacatatta ataacatagt atctactttc gtcatttaaa aattattttc
    19141 caagagcttt ttcaactttg gcataaactt catcacaaat tttcacacca tcttcaacta
    19201 acttatctac ttctgttttt accttattaa catattcttt atcttgtata gaatttatat
    19261 ttataactac atttaattgt ccaccaagta tagcagctct taaacactga actcctacac
    19321 caacatcact tatagcaagt cttgaacctt tatcaactaa atcttcatgt aacttaatag
    19381 cttcatagca aactcttact atgtttaatg gtacttcaca agcaactttt aatgcctttt
    19441 ccattgtttc tgcttttatt cttttttctt cttctgtttc tttaggaagc ccgtaagctt
    19501 ttgataatgg taagaaacat tctgcatcat catctatcat ttttaataaa tctttttcta
    19561 actttcctgc cttacttaat atctctttta cactttcttc atactcagca tacttcttct
    19621 tccctattgt aaggttacat accatactac ctaaagccat acctattgca cctactagtg
    19681 cagcagctcc cccaccacct ggtactgctt ctttggaaga tagtacttct acaaaatcaa
    19741 cacaagtttt atctgctatt ttcatgataa tcctccttga aaggatgtag gaaaatatct
    19801 caaattgttc gagatatttt acgaactatt ttaagatatc tcctactctt tatttataaa
    19861 ttaatttcct tagaataatc cagctattac tccattttca tcaacatcta ttttttctgc
    19921 tgctggaact tttggtaatc ctggcatctt cattatttct ccagtaagtg caactataaa
    19981 tccagctcct gctgaaattg ttgcttgtct tacagttatt ctaaatcctg ttggtctacc
    20041 taatttagtt tggtcatcag ttaaagaata ttgagtctta gccatacata ctggaacttt
    20101 agtaaatcca agtttttcta agttagctat ttccttatca gcttcaggag tataatctac
    20161 accatctgct ccataaatct tagtagctat agcatttatc ttgtctttta tagataaatc
    20221 atcttcatag cagaatctaa agttattttc ttcttcatcc aataatctta aaacttcttt
    20281 agctactgca attccacctt ctccaccttt tgcccatact tcagaaagag caacattaac
    20341 acctaattcc ttacacttgt cttcaacaag tttaagttca gcttcagtat caagtgggaa
    20401 tctgttgatt gcaacaactg ctggtaatcc atatacttga gttatgtttt caacatgttt
    20461 taataagttt ggaagtcctt tttcaagagc ttctaggttt tcgttattta agtctgcttt
    20521 agcaactcca ccattgtatt ttaatgctct tacagtagca actattataa cagcatctgg
    20581 ttttaagttt gccattctac attttatatc taagaatttt tctgctccaa ggtcagcacc
    20641 gaaaccagcc tcagttatta cataatctgc aaagtgcata gccattcttg tagctataac
    20701 agagttacat ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg atggtgttcc
    20761 ttcaagagtt tgaactaggt ttggttttaa agcatctttt aataatgcag ccatagcacc
    20821 attagctttt aattgtctag ctgttacagg ttcaccttcg tagctgtatc caactattat
    20881 gtttcctaat ctttctttta aatcagatat atcacttgcc aaacagaatg ctgccattat
    20941 ttcagaagca acagttatat cgaatccatc ttgtctaact gctccatcac cttttttacc
    21001 cataccatca acgatatttc taagttgtct atcgttcatg tctacacatc ttctccaagt
    21061 tattttctta gggtctattc taagtgcatt tccttggtga atatggttat caagcatagc
    21121 agctaaaaga ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt gtaagtttat
    21181 atcttccata ggaacaactt gtgcatatcc tccaccagct gctccacctt taacaccaaa
    21241 tactggtcct agagatggtt ctcttaaagc aactaataca tttttgtcta gtttagcaaa
    21301 tgcatcagca acacctatag tagtagtagt ttttccttca cctgctggag ttgggtttat
    21361 agctgtagtt agtattaact tagctttttt accagtctct ctttttagta gattgtagtc
    21421 aacttttgct ttgtatttac cataaagttc tacatcatct tcacctagtc ctaatttttt
    21481 tgcaacctct ctaatatctt gaggcttagc ttcttgagct atttcaatgt cagatttaaa
    21541 tcccataact gaatctcctc ccacaaccta aatagattta aaatataatt tttgtaaaaa
    21601 ttgtattaat atgtaaaaat tatattttat aagataatta tacatgtaaa attacttaaa
    21661 aactagctac taagctatta aaatattcaa acttaatagc tagttattta tctaattact
    21721 tacttattac ctatctattg cctatgtcta acctttaacc aacttaaaat cccaaatcaa
    21781 aatttctatc tattttattt atccttaagt gacattaatt tatcttttat tgctttaatc
    21841 tgttctttcg ttgtttcact agaatcatat ggagattgat tagctctatc agaatcaata
    21901 acatcttggt tataataaat aaatcctaaa gattctccat cttctaagtt ttggattata
    21961 aattcttcat cttccttatt tctaatttta tttcctacta caaatacttt atttacacct
    22021 atatcatgtc caagtttctt aacttttcta taagtctgta gacttctctc tcctggttct
    22081 acaactacta taaaagcatc tacaccttgg gctgttcctc ttcctaagtg ttcaattccc
    22141 gcttccatat ccataaccac tacatcttta ttttgtagta ttaaatgtga gcagagtctc
    22201 ttcaaaagaa catgctctgg acatacacat ccagtaccac cagaatctac ggttcccaat
    22261 gtaagtaatc ttactccatt gtattcttta cagaaatttt cagggatatc gtctacttta
    22321 ggattcatct taaacatttt tccaaatgaa cctacagaag cagctgttct atctgaaact
    22381 aattttttca tttctgatat aggtactatt gattcatata cttcttttgg aaaacctagt
    22441 gctaaagcta aattagcatc agggtcggca tcaacagcaa caactctgta tccgtcttca
    22501 gcaaacatcc tagagagcat tgaagaaaaa gtcgttttac ctactccacc tttacctgtt
    22561 attgctatct tcataatccc taccaccttt tattactatt tgctttttag tagttaattt
    22621 tacagttaaa tattttttat actctatatt tttatacact aaaaataaac tacagctagt
    22681 ccttacagga accaattaga tttaccctac tagcttttca aagcagcatg tatagtttta
    22741 ttattcttta atattgttta tattttattt attcagtata ttcattatat tttgatgttc
    22801 tttactttaa tatttgactt tattcaactt tactcaacca gaaacttctt ataaaaacgt
    22861 ttattctata acttcactat tttttaagct tcagcctttt ctgccatttt ttcttcaact
    22921 aatgcttcaa agtgaacacg ttttttctct atatcttcta ttattgtagc tgctaactct
    22981 tttggatttg tatttacagt aaatgtagct ccaactttat cttttaattc actagttaat
    23041 atatccactg ctcttgaaga accagctatt ggaggcatta ttcctaaata agtgtctata
    23101 ccagaagcta caacatagca tcctatagca actgcttttt ctgacatcca ctctggagct
    23161 atacctacaa ctggaaggtc acacatatcc atatctaagt aattagctgc tgctccaacc
    23221 aaatctagta tacgacttat atcaacacaa gaacccatat gtagtactgg tgggattcct
    23281 actaatttac agacagttgc aagaccttta cctgcatatt tttcagccgc atcagattcc
    23341 attaaaccaa acttagctgc tgctgaagct ccacaacctg tagtaactac tattatgtcg
    23401 tttttgatta attctttcat tattgttacg tgagcctcat ttgaaactcc tttagcattg
    23461 ttacatccaa ctacaccagc tgctcctctt aatactccag cttttagaca gtctgttaat
    23521 ggcttaactg ttcctgctgg gtctatatga gagtttacaa ctctgtctaa ttgatttact
    23581 actgcttcta cactataccc aactgttgcc cctgatttta attcaggtat aagtactttt
    23641 gatttatctc tatttttaaa gtttaaaata gcttctttaa ctattgcttt agcatcatca
    23701 agtgcttgtt cttctctaaa ttcaatataa gttgaacctg ttatcttagc ttttggagaa
    23761 gttgttacaa atttagtatg gtaacaatct gcaactctag ctaatgcagg gaatatacat
    23821 tgaacgtcaa ctattacagc ttcaactgct ccagttatta ttgcaagttc ttgttgatgg
    23881 aagtctcctg ctatttttac accatgtctc attgtaatct cattacctgt acaacacata
    23941 cctgctaagt ttattccatc agcaccaact tctttagcta aagctactaa gtctggctct
    24001 tctgatgcta aaactatcat ttctgataat gctggctcat gtccatgtaa tattatgtta
    24061 actttatttt cttctaatac tgctaaattc gcctctgttc ttcttggcac tggagttcca
    24121 aataagatat cgcttagacg agtacctatc atagaaccac cccaaccatc agccatagaa
    24181 gttctaagac tcatgtgtat taaactatct atatcaccag tacatcctat atgagtagag
    24241 tgcattattg ttgcaatttc tctatctata gctcttggct ctatagcgta ttctttccat
    24301 attttttgtc ttggttctgg tgcatttttt ataaatctta atgtcccaaa aggttttcca
    24361 aattccatta atgcaatttc tgccacttca tgtgctacat catagatatc tctaccttct
    24421 gtctctacat cccattcttt agctaaagta attaacttag cttcgtctct tattttatag
    24481 tttccatttg gatcagctaa tgctaatgta tgtgctatat ctctagcatg gtctgaatgg
    24541 gcagctgtcc caccagcaac cattcttgca aagtttctac ctactatagt atgctcatct
    24601 gctccacata acccccttgg agtcttaggg cttattctac atggtcccat tccacatatt
    24661 ctacagcaaa caccttgaag accaaaacca cattgtgcct taaagccttc ttttctatca
    24721 tacattgttt ctacgccttc ttctctagct ttagctatca tcttctggct gtttaaatca
    24781 attgttaaca ttttttcatc catattccta tccccttttt ttgattttta aaatccccca
    24841 ggtgactgac ttcatgtcat aaaataataa aataaatgct ttctaagttg attatatact
    24901 aattttcagt gctttgcttt agatttaaat atttttttaa caaaattaga taatacataa
    24961 aaattttatt ttctacactt aatttaagtt gattttatcg cttgtttttg gctatttgtt
    25021 aatatctaaa cctacttttt tgatacttca gtttaattaa attatgtttt gattttttta
    25081 aatcaaattt gaaatttttc tagtcatttt tatattattg cagattgttt gtgtatttat
    25141 tatctttcac tactgtttgg acttattaca gatttttact ccctaaactt aattagtaaa
    25201 aatttcttag aatagcattt tttggactaa tttttttgaa tttttttatt tttcctactt
    25261 taatattaat tatagcttca aattgaacat ttgtatatat tcatttgaag ctataattat
    25321 tttttatata aaactttttt aatacagcat taatttagac attttgacta ttttttctac
    25381 tttaattcat ttatttcaag cgatttatcc tatttcatat ttttagctac aatagcccca
    25441 tatgcaatag aatataattt agattcatgt gaatcagctc ttgacactaa aactatagga
    25501 gcttttgcac ccataattat acctgctgat ttagagtctg ccatatatgt aaaggctttc
    25561 cctattccat taccaacctc tattgttgga accaatatga tatctacatc tccagatact
    25621 tcacttttaa acccttttat ctcactagct tcttttgaaa cagctaaatc aaaagccaga
    25681 ggaccttcta caattacatt ttcaccaaat tctccttctt tacaagcttt tgctaacata
    25741 tctgcatcta cagttgcttg catcttagga tttactttct ctttagctgc caaacatgca
    25801 acttttacag tttccattcc taaagctttt gcagctttta tagaattttt taatatcttt
    25861 gccttttgtt cataatcagg agctatattc atacctccat ctgtagttat aagtaattta
    25921 tggtattttt ctaactcata taccataaca tgacttaata aactatcagt tctaagacca
    25981 tagtctttat ttaacacttc ttttaatatg acagatgtat ctaataatcc tttcattaca
    26041 aagtcggctt ctttacttga tactaattta actgctattt ctgcacactc tttgatatca
    26101 tccttatcca ctattttgat accacttaaa tcaaatccta tttcttttga tatttctctt
    26161 attttgcctt catttccaac caatatagga gtaattatat ttctttctac agcatccttt
    26221 attgccatta aaacttcttt atcatgagct gctgctacag agagtacaat tttctctcct
    26281 cccttaagtt gttctaaaat atcatctaat ttttttatca aaagtaaccc ctcccttata
    26341 tatatttaac taaataatac catatttgct ataccttttc tcaatttatt ttttatgtta
    26401 taaagaattt atataactaa aagacagttt aatattttat tctgggattt catcaatagc
    26461 tcttaaaagc tttgcaggaa caccacctac aatagtattt tctgcaacat tttttgttac
    26521 aactgctcca gcagcgataa ttgcattatc tccaatagta actcctggaa ctacagttac
    26581 attagcacca atccatacat tttttcctat tgtgattgga gaaggataag ttgtaccacg
    26641 tttctctggt gcaataccat ggttaagtgt agccaatacc acattatgcc caatttgcgt
    26701 attatctcca atctcaatcc ccccctggtc ttgaaaatgg caactgctat ttataaaaac
    26761 attttttcct attgtgatgt tttttccaca atctgtataa aatggtggaa acataaaaaa
    26821 tgattcatct accttttttc caattaactc agaaaataaa ctacggactt cttctggttc
    26881 atgataagaa gaatttaact tacttgtgat tttcattgct tcattactca acttaaccat
    26941 aaattcatgt atttctgatt tactagaaac aggttttctt tgattgatat gtgttaaaaa
    27001 ttcattgata ttcattttat acatcctttc aatatgtgct ttaatttatt tgtacattct
    27061 aaataatatt ttaataaaaa ttaatttaat ttatttttat taaaatatca ttgcttcttt
    27121 tagtgtaata tacaattata tctatatcaa atatctatat taaatactta actatactta
    27181 aaaagcatat aaattgatat aattatataa tagataagtt aatattgagg agggataatt
    27241 atggaaataa gagtattaag atatttttta gcaattgcaa gagaagaaag tatatctggt
    27301 gcggcaacct ttttacatat aacacaacca actctttcta gacaaattat ggaacttgaa
    27361 gaagagttgg gagttaaatt attggttcga ggaaatagaa aaatcacctt aacaaatgaa
    27421 ggtaggcttt tacataaaag agcagaagaa attatatcat tagttgataa ggttgaatta
    27481 gaatttgaca atcccaatga gattattagc ggtgatatat atataggaag tggtgaaact
    27541 gaagctatgc gacttatagc tagaactgcc catcattttc atcaaaaata ccccaatgta
    27601 agatatcacc tttttagtgg gaatgctgat gatgtgactg aaaaattaga caagggactg
    27661 cttgattttg gtattttaat tgaacctgca aatatacaga agtatgatta tgcacaaatt
    27721 cctgctactg atgtatgggg gttactaatg cgtaaagata gtgcccttgc atcacaaagc
    27781 acaataaatc caaaagattt atgcaatata cctcttatta tttcaagaca gacacttgtt
    27841 gataaagcaa tttctaaatg gcttcaagac agtaatgaaa aactaaacat tattgcaaca
    27901 tacaatttag tttataacgc ttctcttttg gtagaagaag gtctgggcta cgctctgtgt
    27961 ttagatagac tcgtcaatac tacagaaaac agtaatcttt gttttagacc tctatctcct
    28021 cctttagaag ctaaattaaa tattatatgg aaaaaacatc aaatattttc aaaggcagca
    28081 gaaaaatttt tacttcaaat acaaaaagat ttttcttcta tcatgttgga tacatctaag
    28141 ttggatacac ctaaagaaaa ataaatcata ataaaaagtg agagtgtctc aaaatgaact
    28201 ttgcactgta taaggcactc ttttttatac aaagtaaaat atatgttcat catttcagca
    28261 ctaaaaaaga ggcttatctc tattttgaga tagccccact taaccaataa aattaaatac
    28321 aacttaacaa tatccttact tatttattcc aagttctctt agacaagcaa tagtaatttc
    28381 atcttgtgga tttaattcat aagctctttc tatatagtaa gttgcatcat caatatcacc
    28441 attatttaaa taagccattc ctaaattgca aagtatttct gggtcttcct ttatcttagc
    28501 cgcttgttca aaatactcta ttgctttatc taaattattc ttataagcct cacaaagccc
    28561 caattccact atagtatcca cttgattagg ctttattatc aatatcttct caagatacat
    28621 cttagcctgc tctatttcgc ccataccctt atatccaagt gctatcataa acagcaagtt
    28681 ccaccaatcc atatgttctt cttctaatgg aagtaacttc tctaatcctt ccttaaactt
    28741 accttggaat acaagtgtat atccttcttc atattgaact ttaaagtcca tcttgcccag
    28801 attctcttgt acctcagcta ctaaatcagc atctaaccct agccttaacg cttcttccca
    28861 agtcagctta gtctttaaat attgaccttg attataataa tggtagccta agtgataata
    28921 acctaatgca aaattctcat caacatcaat taccttttca agcttagcca tggcttcaag
    28981 taagaatgca ttcattgcct tatcatccat atctttttga tactgtttag ctatctcttg
    29041 gcaaacaatc gcatagttat acattgcatc taaatcatct ggatacattg ttataaatgc
    29101 ttttatatat attaaagaat ctgtatgttc accaatctca aagtattttc tcgacatata
    29161 tcccatatac gatttcaaat ccaaatcaat atcttttgat aaagcattta aaaacttctc
    29221 atactctgca ttgtttttaa acttactatc tattccaatt atatatatca ttgcatctgc
    29281 tatagacatt gaatttaact tatcttgagc agtcttttct tttatccctt ttactaatac
    29341 ctcatttttt ataggtacat ctaatccacc ttttggcact tcatagcttt ttaatttaaa
    29401 ctctccacca tgttttatag ttataaaagc taattcttct gcttttttta gcaaatattt
    29461 ttctatctta aaatccattt tatcctccta aatacttaaa tctctacttt atgagtttta
    29521 gatttctcaa atattttttt atcatcatac tcaaataaga ctacattttc tacttctctt
    29581 atttctgtat ttatatcatt ttttaaatcc acaacattaa atctaaatgg ctcaaaatga
    29641 acatatttta ttatatgttt agctggttta tcaacaaaac taggtaaata tgttgataag
    29701 ttatcattat cttgtaaaaa tacatggcat ctattcttaa aatcatccac atctagctta
    29761 ttaaaaaact gtggtatatt tgatgtttta cctagtgata tatagtcata ttttaatata
    29821 tcgttgaata catcattatc tatgttgatt ttttcattat agaaatccat caatatttta
    29881 tataactgat tctttccttg tgccaaatca aagtagccat tttcatcaaa atacttggca
    29941 aaaacctcaa agaatttaaa agggcttttt ttataaaatc tgcctataat atatctcata
    30001 gataacacaa agtttttaga attgtagtat ctctccagta tatcttctat atcctttagc
    30061 ttcaaagttt cttcatagct tatataatta ttgtataaaa actcatatgg agcataatcc
    30121 ttatatttaa aaccatgctc atctgcaact tttctcatac ctgtcccttt aatcatcttt
    30181 aaaaagccta attgcagatg ctctatacct agattaaata catcattaaa agaattttca
    30241 aagctcttat aatcttcata aggcagtcct gcaattaagt ctaagtgctg atgtatattt
    30301 ctataagatg ctacagtttg tacaacatga gacaactttt taaaatcatc ccttctgcct
    30361 actgcatcaa gtgttttttc attcgttgac tgaaccccta tttcaaattg aaaaagacct
    30421 tctttacaat cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc tgtaacttca
    30481 aaatgataag ttgtatatcc attatcattt ttcataagga aatccatgat ttccatagcc
    30541 acttttctat ttgcattgaa tgtcctatct ataaacttta tttgtgatac tcttgcatct
    30601 attaaagctt ttaaatcact ctttatcctg tctaaactaa aataccttag acctggaata
    30661 gtagatgata aacagtattg gcagttaaaa gggcaacctc ttgatgattc ataatacact
    30721 attctatttt catattcttt tggattcaag ttttcataag gactaggaat ctcatccaaa
    30781 tcttgaagta attccatagg cttattcact attatctcat tgccttgtct atatacaagt
    30841 ccatttacat catttatttc catctttcct tgcaagtgaa gtactaagtc tctaaaaatt
    30901 aattctccct ctccataaag tatataatcc acaaaatcat actttttcat tgcctcataa
    30961 ctatcataac tcacttccgg tccgcccaaa gctattttag tattaggtct aatttttttt
    31021 agattatcac aaagtttaac tatatctccc acattccaga tatatgttga gaataatatt
    31081 atatcatatt catttttata tatatccttt aaaatatagt ccaaatcgtt atttatcgtg
    31141 tattctttca tattaacttc tatcaaatct cttacaaatt cttttagata tcttatagcc
    31201 aaatttgtat gaataaactt ggaatttaat gttgttaata aaattttcaa atatttctca
    31261 cctcttttta agattatact gtctttttgt accccttata ggttaaaata gtatatgtaa
    31321 agtctaaata atatctatat aattaattta ttaatttcaa gtaatgtgag gaaaggaagt
    31381 tttttatgga attaattgaa aaagtatcta tcaactcttt atcaaaaaga gatttactac
    31441 ttattataaa gtctcttgaa tacactaatg aaaatactca aattagtgat ttcattgacc
    31501 ttaggaataa tatagtaaag gaattatgtt ttttaactga cactaaggaa aaagattttt
    31561 tagattatct agaaaaaaat agcacactct aataacctaa acctttttaa actttaatat
    31621 ataaatacct gatttagaat ttatttttat gcttaatata ttatcattaa aggttttcga
    31681 cataactgtt atttgattaa atatagggtc tgctatagta ttaccttctt caagtcttat
    31741 aagacttctt gggtctatgc ttttattata tgccttaact ccaattttat attttctcaa
    31801 tttaacaaca tattcggaca tagaaaaaat aaaatctata ttatcttcat ttaatttact
    31861 atcttcaccc aaaatggata atatagattt ttttatgcta ttaattcctt cattttctac
    31921 agttctatct tccatatata catcatatag aacatttaaa acatttaata aaatactttc
    31981 ttcatcatga tcttcagatt tcaaatcaat gtacttatca attaattgta ttgctttaat
    32041 ccttgccatc tgaaacttta caacatctga tttgtctaat tctatagaaa atcctattat
    32101 cttgtcttta atactctgta gtaattcgac atactctatt ttactattat ttataagatt
    32161 gtgcataatg gaattataca ccacagaacc taataaataa tcaaataaaa gttcttcttt
    32221 tttaaaataa ataacatact tttttattag tttaataacc tcatcttcac ttttttgaat
    32281 attagtattt gataaaatta gtggtaataa aatatttaaa aaattgatat ttcctatttt
    32341 ttctccataa aatctatagt aaataagttc atctctcagt ctattttcgt ctatatacac
    32401 acatctttgc gttttatcat ctattaagga ttttgtattt attatagtta aatctttaag
    32461 agccctactt gcactactaa aagaagatgt gtagactttt tttagtaatt cgtcattcat
    32521 aaatatatcc tttctgaaaa attgaatgta aataaattgt tattaaaaaa aaagagacag
    32581 cccgtttaag aagctgtcat atataggggg atagtagtgt acaaaaatca ttgtaatcta
    32641 ctatctatat attattatac gatactactt ttttatatgt aaagcatcag ttttcgacat
    32701 ccctatattt tacagttata caaattgcgt actaagttca attttattct ttttatttca
    32761 acattttatc atgctacatt ctttcaggag cgtgcattcc caacaatttc agagaatttt
    32821 ctaaagtttg ttttgttgct aaaactaatg caagtcttgc ttttcttatt tccaaatttt
    32881 ctactaaaat tggattgtca tgataaaatt tattaaacgc ttgagctaaa tctagcataa
    32941 atctagtcac tatatgtggc tcatttttct ttagagcaag taaaatattc ttattgaatg
    33001 attctataac tctaagaact tctgcactat cttcatcagc taatagacta taatctatat
    33061 cagaagtcac ttctacttct gcttttctta aaactgcaca gcatctagca tgtgtatact
    33121 gaacatatgg tccagtctca ccatcaaaac taagcgttct ttcccaagag aaggtatagt
    33181 ctttaattct actattagat aactcttgga atactactgc cccaacacct acttgcttag
    33241 atatttcatc cacattttta gcatttgggt ttttagctag tataatttcc ttagttttat
    33301 ctatagcttg atttaaagca tcttctaaaa atactactct tccttttcta gtcgacatag
    33361 taccttcttc aagtgcaacc ataccaaatc caacatgaat taaatctttt gcccaatcat
    33421 atcccataag ctcaattacc ttaaaccatt gttggaaatg taagttttgt tgagaaccaa
    33481 ctacatatat acacttgtca aaatcataag tttcttttct atatattgca gcagctaaat
    33541 ctcttgtcat atataacgtt gaaccatcat tcttagtaat aagtgctggt ggcattttat
    33601 actcttctaa atcaacaatt cttgctcctt tagactcttt taaaagattt ttctcttcta
    33661 atagttcgat aactctgttc attttatcag aataaaaact ctctcctgct aaagaatcaa
    33721 attctatatc taaaagttta tacactctgt taaactcttt taaactctca tttctaaacc
    33781 attgccataa ttcttgtgct tcttcgtcac cattttctaa tttattaaac catgctcttg
    33841 cttcatcttc catttctggg tgttgttcag cttcatcatg gaatcttacg tataatttta
    33901 gcaattctgg aattggatta gactctacta cttctttttc tccccacttt ttaaaggcca
    33961 ctattaactt accaaactga gttccatagt cacctaaatg atttattctt atagtcttgt
    34021 atccttgcga atcatatatc ttatataaag cattacctat aactgtagtt cttatgtgcc
    34081 ctatatggaa aggttttgct atatttggag aagaatattc aactattaca gttttatctt
    34141 ttccaaaatc actatgtccg taattttctt tttcgtctaa aactttttta attacagttt
    34201 ctgctaattg agatttattt acaaagaaat ttacatatcc gcctaattgt attacttttg
    34261 ttatctctcc acttggttct atactttccg ccaattcact tgctatcatg tttggagctt
    34321 ttctaaacac tttggctaat ttaaagcatg gaaaagcata gtcccccata tccttatttg
    34381 gtggaacttc tataagtgcc tctatctctt ctttcgataa atcttctatt ttttctttaa
    34441 ggcaatttga tattgccacc ttaaaatctt gcattctgtt gctcctctct acattgttcc
    34501 tggtttatat tgtgcttatc tctttatgtt aaatcaaatt tcgtgttttt tcaagtattt
    34561 cattacagtg taaaacactt tagtaattat gttttctgta tatattaaaa atactataat
    34621 tgcagttttt aatcacacta ttattgattt tttataagac ttttttctat tttattgttt
    34681 cttgtactat ccagttgact ttatatactt tttttactct tttatctgtt ttcatacatc
    34741 aatgtcagct ttatatacat ttttttaatc ttttatctat tttcatacat caatgtcagc
    34801 tttatatact ttttttactt ttttatctat ttttatacat caatattagc tttatatact
    34861 ttttttactc ttttatctat tttctataaa tctttttcac tcttaacttt tattccttct
    34921 tcttctaata gccttgctgt tatacctttc cctggtatct ttttacctga aaaactacca
    34981 tcatatatta gattacatcc acaagagggg ctaccctctt ttagtattgc ttcgctacat
    35041 acatataaat ttgctatttt tagagtttca ttagcccctc ttacaaagtt ttctgttaca
    35101 tccttattat ctttagtaat tacttttcca tcaactatct cagcaggtgg tcttggtgta
    35161 gatagaccac ctaactgctc tgggcataca agtgtaaatt ctttgccttt taaatattcc
    35221 ttgacttttt cattatcatt attatcgcca ctatatttac aatttattcc tattaaacat
    35281 gcagatacca atatcataaa aatgtgctcc tatacaaatt atatatattc tcatacaaat
    35341 tactaatgta ttcttataca aattatttta atttaacaat tgttactcct gctccacctt
    35401 ctccatattg accacctctt tgtgatttta catgtctatt tcttcttaaa atgtcttgta
    35461 gccctgcttt taatactcct gtacctattc catgtattac tgttacagac tctaaacctg
    35521 ccacatatgc atcatcaaga tacttttcta cttccattat agcttcttct aggtttaacc
    35581 ctctaaggtc aacttcattt tttacactac cagattttga ccttataatg tttctcgttg
    35641 atttagttac atttgttgaa acatctttgc gtgttttttg taaagattta aaaggtagag
    35701 tcatcttcat tataccaatt tgcactactg cttcttttct ctttttatct actgaaacta
    35761 cactaccatt ttgatttaaa gttattacct taacttcttc acctggcttt aagtctttta
    35821 tctccttatt tgataccttt ggaactatca tactttttac tgttggctga agacttccca
    35881 tagagctagt caattctttt ctaagttctt ctatcttacg attcttttct tttgaagctc
    35941 tttcttgttc aagacttcta agttctttta ttataatatc tacttcttct ttagcttgtc
    36001 ttataattga aaatgcttct gatttagctt tttcaatcat cttatctctt tgactgacca
    36061 atttttcaag tttttcgtca tactctactt tcagtttttc aatctcttct tttaatcttt
    36121 cagcttcttc tctatcttct actgctttga ttctattctt ttcaacattc tgtaacacat
    36181 cttctaaagc tatgttttct gtatttatat attctttagc acgtgaaatc acataatcac
    36241 ttagcccaag ttttctagaa atctcaaacg catttgattt tccgggaaca cctataagta
    36301 acttatatgt tgggcttaaa gtttctatat caaattcaac tgctgcattt tctacacctg
    36361 atttagtaag agcataattt ttaagctcac tatagtgagt ggtagctata catttagctc
    36421 ctacagaatt tatatcttct aatactgcta ttgcaagggc agctccttca acagggtctg
    36481 ttcccgctcc taactcgtca aatataacta aagagtctgc tgttacgttt tgtaatatag
    36541 atactatatt cgtcatatga gatgaaaatg ttgatagact ctgttcaata ctctgctcat
    36601 ctcctatatc tgcaaataca ttatcatata cacacatact acttccataa tcagcaggta
    36661 tatgaagtcc actttgtgtc ataagtgcaa atagacctac agtttttatt gtaacagtct
    36721 taccacctgt gtttggacct gttatcacca atgtgtggaa gtctcttcct aaataaattg
    36781 tatttgctac taccttcttt ttatccaaca gtggatgtct tccatttttt atgtttaaat
    36841 atttatcctc atttaaagtt ggttctattc ctctcatctg tatagaaagt tttccctttg
    36901 aaaatgcaaa gtccaatctt ccaagtatct cttgattaga gattaaatct tcgctcactt
    36961 ctccaaccat agcagaaagc tctgataaaa ttctttctat ttcctcttgc tctcctagtt
    37021 tcaactgcct caattcattg ttcatctcta ctatagtcat tggctctata aaaagtgtcg
    37081 caccagaaga tgactggtca tgcactatac cagctacttg cgacctatat tcagctttta
    37141 caggtactac aaatctatca cctcttaggg aaattattgc atcttgtaag tacttttgat
    37201 aagtagttga acttattatt gaatttagtt ttgacctaat ggattgattt ttttgagata
    37261 ttcttcttct gatatctcta agtgtacttg aagcattgtc agatatttct acttcactaa
    37321 ttatagcatt gtatatctgg tcttctatgt ccttgtatac atatagagaa tttgatagag
    37381 attgtattat tggataatta aaatcttctt catcactact agataagcta tttcttaatc
    37441 ttctagccac tcttaaggta tctgctatca taatcagact tcctgggtca agagaagcac
    37501 ctatataagc tctctttacc ttatcttcta tatcatgaat accttccaac cctactgaac
    37561 ctctctttat aataattgat tgagcttcac tagtttcttc tagcatagat ttcacttcta
    37621 caaaatcagt atttggtact aaattttcta tatattttag acctaaagaa gatgaggctt
    37681 tcttctttaa caaatctatt attttattgt attctagaac tcttaacgat ttctcattca
    37741 ttttctatat tcaccatcct cgatatttta acttttttat ttaattttaa aaatgtatgt
    37801 tttcaagcat ttccaataaa aactgcaagt tttatttctt tttcatttct aaaattatta
    37861 tattttttct cttttagata tattttacac atattttgta ttatactatg tatgtagcta
    37921 tctatatatt atacataaac atgctttatt tgtaatataa tctgttaaaa aatcttcttt
    37981 aatattttta aaggagttta ttttagctta taaaaaaaaa tagctactta tatagataaa
    38041 ttaaggagga acatgagtca tgggaaacgt attagaagga ttaaaaccag aaagtgtttt
    38101 taaaaacttt gagaaaatat ctcaaattcc tagaggttct ggaaatgaaa aaggcataag
    38161 tgatttttta cttagctttg gtaaaaattt aggtcttgaa actattcagg atgaaagctt
    38221 aaacataata ataagaaagc ctgctacaaa aggatatgaa aattgtccag gtgtagttct
    38281 tcaaggacat atggatatgg tttgcgaaaa agaaaagaat gttgaacatg attttcttaa
    38341 agaccctata aaattaagaa ttgatggtga catgatatat gctactggaa ctactctagg
    38401 agctgacaat ggtatagctg ttgctatggg tatggctata ttagaagata atactttaga
    38461 gcatcctgca ctagaagttt tagttactgt taatgaagaa gatggtatga atggtgcaga
    38521 tgcactagac cctagcttaa ttaaaggtca atatatccta aatatggact ccgaggagga
    38581 aggatactta ttagttagct gtgctggtgg taaaacttgt gttgttagtt tacctgttga
    38641 atacaaggaa gttaaaggtg acaaacaagg attattagtt gaggttactg gactattagg
    38701 tggacactct ggtatggaaa ttgttttaca aagagcaaat gctaacaaag caatagctag
    38761 agtattaagt gtattgaatg ttgattatga attagcttct gttgatggtg gtactaagca
    38821 caatgctata cctcgtgaag ctaaatgtgt aatagctgtt aataaagctg atgttgaatc
    38881 tgctaaaaaa caaataaatg acattttaac tgcatttaaa catgagttta ctacttctga
    38941 ccctggcatg acttactcag tagctgaaac ttctgtagac aaagttctta ctaaagattg
    39001 taaagaaaaa gttgttcaaa tgtcttgctt aactcctcat ggagttcaat cagtaagtct
    39061 tgatatagaa ggattagttg aaagttctac taacttcgct ataattgaaa ctaaagaatc
    39121 tactattgaa tttttaactt ctgttagaag ttctgttatg tctattagag atgaaatcgc
    39181 tgatagaata agacttttgg ctcaagcttt aggtgctaat tacgacctta tagctcaata
    39241 cccagcttgg gaatttaaaa aaggctcaaa attagaaaaa atatgcagtg aaacttatga
    39301 aaaattaact ggtaaagttc ctactgttat ggctctacat gctggtcttg agtgtggatt
    39361 attacttgat aaacttcctc atgctgaagc tatctctata ggaccagata tgtttgatgt
    39421 tcatactcca aatgaacatg ttagtatccc ttctgtagct aatgtttggg attatgttat
    39481 tgaaatatta aaatctatga accaatatta aatttaagtt ttgattctaa gtagatttta
    39541 attttaagta atctatataa aattaactct gtaaattaat actactgtta gtataatatt
    39601 tactttcaat attaacttat agaaataaag cctaatgata gataataaca tgtatcatta
    39661 ggctttattg taatttaaat attattaagc tttattctgt tttaaaatta attcttatac
    39721 tattttaaat ctatttcttc cttgtctctt agaatcataa agagcattat ctgctatttg
    39781 atacagttca tcatactctt gaccaggctt tgctataact acccccatac taatagataa
    39841 actttctcca tttggcaact taatgagatg aatatgattg tatacatcat tacatttatt
    39901 aataagttct tccttattta ttatatcatt gatataaaca ctaaattcat ctccacccaa
    39961 acgacctact atatcatttt ttgcaaaact ttcatcaagt acctttgcaa catcaataag
    40021 tactttatct cctaacaagt gaccataagt atcattaatc gacttaaaat aatcaatatc
    40081 tataatcaat aaagcaccat ttttttcagt tgataaattc aacaaatttt gtgcagttaa
    40141 ttttctaata tatgctgcat tataaatatt ggttaaacta tccctttgag ccttatctaa
    40201 caatctattc ttttcctctt tttcagaatc aatattacat attttcccga ttacatacat
    40261 tggtattttt ctatcattat aaattatttt tgaagtaact ctaatccaat gaaaagtatc
    40321 atcaagcata atagaataca tttcagttgt tccattttct tgtgatttta ttacatcaaa
    40381 aaacttttta ttatattctt tccttatttt atctctttca ttttctatat tgccattttc
    40441 actttcctta tcatttttat ccaaaccata ataagtttca ataccctttt ctggcataga
    40501 aattatcaat ttatcttgct tataatcata ctcaaaaaag tattcacttg tcatttcata
    40561 caactgagta tgttttttga gttccaaaga tatcttctcg ttcatacttg tacgagtatg
    40621 taaaccaaat gccataaatc caataataca aattaataaa ctggctacaa taaaaatagt
    40681 ctcaattgga ttctgttgca taatataaag caacgagaaa tcttgtctat atgtagtatt
    40741 ttggtagaca attgattgca ttttttctga tgaaatactt ataatagctt tattaaatat
    40801 attcaataat tcttgtttat cttttttaat tataccaaaa caaatactct ttgctttata
    40861 ggtttgagga actagacgca catttttata cttaggttga ttcatatagt actgcataga
    40921 ataaccttct ccataagtgt aatctgcata gcctttatta acagcttcta tacactcttc
    40981 taaattatca taccaaacta tattctcttc aaactggcca taataattaa cacctttggt
    41041 tagagctata cgttttccat ttagactatt ctcatttaat ttattattaa tcatcatagt
    41101 atattgagta gaaagaaaag gccgagacat agctatatta tgttcacgag agtactcata
    41161 atcataaatc attcctgcta caatatcaat ttcatgtttt ttagccatat cttccaaatc
    41221 ttcttgagat tttacaggca caagttcaaa ttccaaacct gttttatcac atatatgatt
    41281 taacaaatca attgatatac ctcttaattc tcctgtatct ttatctgcat tttgaaacgg
    41341 tggtttgttt aaaagtactc ctacttttaa tttttctgtc tctttaatgt aattagtttc
    41401 ttcatcagat aaaaacattt gattattctc tggcgtaaag tatttctcat ataaagtagt
    41461 tgaaaaatat gggtccttct gttcaatatt attaattgct gaattaagct tttgagttaa
    41521 gtctgtactg ccttttgggg taatgaaata aaatggcttt gatgagaatt ttgctacagt
    41581 acgtacacct tctattgggt tcatactaac attaagaagt acatctgctt taccactctt
    41641 tagtacttct aattgctcgt catttgtttt acaatatacc aattcaggtg acatcagatt
    41701 catcttacaa tactcatcta actctttaag cctagtatca gaattttcta ttacagcaat
    41761 acggattttt ttcacatctt gtgaactcat tatgttatca atatctgtat tttcatataa
    41821 tgcttgtagt actgtatttg ctactccata attattccct gcataattat atattttttg
    41881 cattggctca ttgtataaca tccctcccat tatatcaatt tcaccatttt ctaacatctt
    41941 cattaatttt gtaatagatt catttatctc tccaggaact tgaacaaatt cataattcca
    42001 tccagtatac tgtgcaattt ctagtaaata ttcatatgta tacccaaaat agttaccttg
    42061 ctcatctatt tcagttaaac ctttttgaat aggataagca acacgaatgg tacgattatc
    42121 attacctata gcgttgatac caaaagaaaa tatgcaagaa gtgaatatag atgtaattac
    42181 aataattaat aaaagtaatt tttttaaatt cactacacaa tcctccatat agttaagatt
    42241 tacagtataa attactgcat aaacttgtag acatgaaaaa atagattagt atataaaagg
    42301 atatataaat ctacttagag atttaaatat ccttaatata tacaatatat ttaaattata
    42361 actcatttat atgtctatta caacaaattt accctgctaa atacctactc tatatcccat
    42421 aattttacaa gttgtagtga agtttaagtt ctatctcacc tcaaatttag cagtgtttct
    42481 atttacagtt cttttatatt tcacatctgg ataacccaat ataaatgaag tgataactgc
    42541 ttcgttctcg ctcattccta acagttcttt tatctttggc tctacatttg atgccataac
    42601 aaaaaatcca ttataacaaa ttcctaatcc ttgagaatag gccataagtt ccatattaga
    42661 tgcagccaat cctccatcta cataagcact tccacctaga gacatatcac ctacaactac
    42721 cataagtagt ggggcatcat aaaataaaga atcatttccg ttctctttgt atcttttgta
    42781 cattttctta aatgtatttt tatacataga acgtacaggg tcattgtcat cagtatctaa
    42841 tgccaaatta taaagacctt gtattgccag ttctttcact tcttttagct tttcttttac
    42901 taatatgtat cttataggct gtctattacc acctgttggt gtgtatctac cagcttccac
    42961 tatgtttttt attttttctt cttctacctc tatatcttta tattgtctta tacttcttct
    43021 aaatttaata aaattcaata acttttcggg ttctaattca aaactttcac gattgtattc
    43081 tattacttca tttttatcaa atccttgcat ttctattgca tctgttgggc aaattgccat
    43141 acagtgacca cagttaatac agagaatatt tttaaccttt gctttatcat ccttaacttt
    43201 tattgcattt acaagacagt ctgattcaca catcccacag cctacacatt tttctaaatt
    43261 tatgtctatc attccaaatt attcctcctt ttaaaaaatt tactttagta taaaacaaat
    43321 agttgtctaa gtcaattttt atttttttaa caatatacta gccttaacta cactttaatg
    43381 gagaaaaata tacataaatt ggaacttaaa ttagaactta ttcaaagtaa aagataaaag
    43441 aagtagatgt ctaaaagtga actttttagt taattagata ttctttattg tatgaaatca
    43501 agtggatatt tatcatttca acaataaaaa aggcttacct ctattttgga tatgtccctg
    43561 tcaagtagac atactaaaaa aaggctatct ttatgcactg agatagcccc ttttcccaac
    43621 tactattgtt tatcttaata ttagtcaata aatttaattt gctataacct gtaagttaca
    43681 ttttaatttg atgtaacttt aaattacatt tttaagtaat atctttaaat tatattctta
    43741 aaatatatca ttaaactaca ttttaattaa tctaaaaact atatcttaat taatctaaga
    43801 aacttacatt ataatctttt agtttcccat tatcatatac aatctttgca ctaaagatat
    43861 ttccagtctt agcccaattt aagaacagcc tgtcaccttc ccacatattt aaatcgaata
    43921 ttttatcatt atcaacccaa attaaatttc cttctttaca atcatttaaa tctttttcag
    43981 ataattcacc ttcaaactca tctgagaaat acaaaaacat ataccaatct tcttcatctt
    44041 taaattttgg aaaagttatc aaccctttat atgacaatga tttaagagta agacctgtct
    44101 cttcttttac ttctctaatt acacattctt ctggagtctc tccttgctca aactttcctc
    44161 caactcctac atacttacct tcatgtatat catctttctt tttatttctg tacaacatca
    44221 aagtcttgtc atctttttct atataacaaa tagttgtaag tttcatgtat ctcgccttgc
    44281 tttctcttta tattctcaac taattatttt cagcactttc aagcacatca cttatattct
    44341 tcattgcatt aagagaaagc tctgcaaatt catcaaatgg aatccctata tcttctatac
    44401 ttctcattgc atctctattt acatttgaag caaatctcgt ttctttcatt cttttaataa
    44461 cagacttagt ctttacactg gtaagttttt tatctggata aactaaagct atagcagtta
    44521 taaacccagt tatagggtct gctgcatata ttgctttttc cattaggcta gttcttgtaa
    44581 ctcccattcc atcatgatga ccaagtattg ctctatacat ttcttcgtca ccgaagtttt
    44641 cttttttaag cagttctact gttgttgtag catgtccttc acatatttct cctggctttc
    44701 ttccaactat atctgaatcc aaatcatgta aaagacctgc cactgcccac tcttcttctt
    44761 tttctggctc tagtcttttg gctaattctc tcataacagc ttcaacagca taacaatgtt
    44821 ttctcattct atcagtttct aaatacttaa ataaaatttc taaagcttct tctctcattt
    44881 tcaccaatcc ccctgttttt tcacatatat gatattttta tatattaata cacataaatt
    44941 caagttttta tatatattca tgtatatttt tcatgttata tctattctac acctttatag
    45001 tagtgtcctg caactgttcc aatttcatct aatttcttat acaagtctgt agcctttttt
    45061 ccaagtttat aattattact caatacctca atgtgagctt caataacctt tttaaccatc
    45121 tcactatctt caactgtaaa atctatccta aatacatcta ttccaatttc ctctaactca
    45181 tatagattgt ccaacataca agtaaccttc gaattgtaaa ttgtacttct acagaatata
    45241 tcttgtgtta tcctataact tgcatccgta aagtctctta aagcataact actttcctta
    45301 catttagcaa ctctcttgtc ctttttacaa tctctaacaa ctactcccat aggacagtat
    45361 tctgtaacca tcataggagc atatccatat attactgctt caatttctac atctgtatat
    45421 tttaaagttt gtttaatttc atttaagttt agttcttgtg acaaacaaac tgtactcgct
    45481 ccctcttctt tgaagtgatt tatactttca ctattgaatg catttaagta gcaatctata
    45541 tttaagtctt ttttcttaaa gtattccata gagccccagt taccaatttg aaccttatta
    45601 attcctttta tattatccaa tatctcaaat tgcttgtact ctttatttct aacaatcctt
    45661 ggtgctgaat agattaattt tttgtcagtg actgctgtat tagagtggtt cttattcaaa
    45721 gtatctattt tacttgtatt tgacaaagta tctatgttat ttgtatttga acccattagt
    45781 tctttaggag tacctaaaga cgaagaagta tctaaatcca tagaaatact tgaatctata
    45841 gaaatatttg aatctataga aatacttgag accatttgta aagcttttga taatgtattt
    45901 atatcttcat aatagattaa atctacatta taatttaata ctgcttctaa ttgctccaag
    45961 ttcttaactt taactctaat cttaggagct gtatcaagtt ttttctgttt atttactcta
    46021 tttggctggt attttaatct attcttctta aattttctat cttttatctt tattctttct
    46081 tcatctaaaa gctcaataca atcccttctc atctgattta acacacttat aggcatactt
    46141 acaccttcat ccaggtcaat ttctatctct tttaacttat atggtgtatt tccaagcttt
    46201 tgaatttgtg tttctatttt ttctttactt aaagccacct ttatagcttt ttcaactatt
    46261 ttgtctcctt ctaccaccga tttatttcca caaaaatcac ttataaaaag ctctggcttt
    46321 ttacctagtt taatactcac ttttgcacgt atattagttt ttatgaactc tttatcttgc
    46381 tgaaatgtag cttgtactct atccattaat tcgctgtcag aagtcttata tataagctga
    46441 tttctctttg cttctccaac aaaatctaat tctatcaaat cccctttatg tgctattgta
    46501 gttatctctc catcctttat tattcttcca atagtacctc cacctaagtt aattccatca
    46561 cctttcttaa gcgtattttc tagcattact ctaagtcttt tgttctttct attataatct
    46621 aaaacttttc caatatatag cccttgatta ttaggtctat tagagttcat tatatcctta
    46681 ccaacttcac ctaatatata tccttttgta aactttctat taaatatggt atacaaatta
    46741 tttattattt cctcagaaat acttattttt ttcttatcta caaattcatt aatcgcttct
    46801 ctatatcctg aaaccacagt agctacatac tctggctttt tcattctacc ttcaattttt
    46861 aaagataata cacctgcttc aattatttta tccaaatctt ctatagtact taaatctcta
    46921 ggactcaaca aataatctcc attacttttt actacttctc ctgtatttat atctatcaac
    46981 tcatactttt gtctacatgg ctgtgcacac ctacctctat ttccagacct attgcctatc
    47041 atactactca taaggcattg acccgagtaa catacacata aagctccatg tacaaaaact
    47101 tctatatcta cagttgtatt ttcacaaatc cattttatct catcaacatt taattctctt
    47161 gctaaaacaa ctctatcaaa ccctacactc tgaaggtatt ttacatcttc taaggaatgt
    47221 gctaccattt gagtgcttgc atgtagttca aagtcaggta atagtttttt tataagtctt
    47281 gccattccta tatcctgtac tattaaagca tcaatatcta tatcgtataa aaattttaca
    47341 tactctacaa aatcttctat ttcatactgt tttatcaatg tatttacagt aacaaagact
    47401 tgcactcccc ttatatgaga gtatttaaca gcctcaatca gctcatttct atcaaagtta
    47461 tttgcagatg ctctagcact aaagtcttta ccacctaaat acacagcatt tgctccgttt
    47521 tgaactgctg cttttaatga atcaaaagag cctactggtg caagtaattc tatttttttc
    47581 atatttttct cctctattta tacttgttag gttaatataa ttatatctat aacttaaata
    47641 tttgtattaa atttatatat ttcttactat attttaaatt cttcaatcaa tttatatatt
    47701 agttatttat atttggttca tttgagttaa catatacatt atttactata tttagtatat
    47761 ttctattaac ttatatattg attactgtat tttaaattct tcaattaatt tatatactgc
    47821 ttactaaatt taaaacattt taattaattt
//
LOCUS       DAFKMX010000007        45145 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFKMX010000007 DAFKMX010000000
VERSION     DAFKMX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.007"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(363..2123)
                     /locus_tag="LZB08_000311"
     CDS             complement(363..2123)
                     /locus_tag="LZB08_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBM7313832.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(2186..2329)
                     /locus_tag="LZB08_000312"
     CDS             complement(2186..2329)
                     /locus_tag="LZB08_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7313833.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(2648..2887)
                     /locus_tag="LZB08_000313"
     CDS             complement(2648..2887)
                     /locus_tag="LZB08_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313834.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(2892..3308)
                     /locus_tag="LZB08_000314"
     CDS             complement(2892..3308)
                     /locus_tag="LZB08_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7313835.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(3305..3604)
                     /locus_tag="LZB08_000315"
     CDS             complement(3305..3604)
                     /locus_tag="LZB08_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7313836.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(4655..4822)
                     /locus_tag="LZB08_000316"
     CDS             complement(4655..4822)
                     /locus_tag="LZB08_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313837.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(4835..7600)
                     /gene="mgtA"
                     /locus_tag="LZB08_000317"
     CDS             complement(4835..7600)
                     /gene="mgtA"
                     /locus_tag="LZB08_000317"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBM7313838.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(7766..8077)
                     /locus_tag="LZB08_000318"
     CDS             complement(7766..8077)
                     /locus_tag="LZB08_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBM7313839.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(8324..9001)
                     /locus_tag="LZB08_000319"
     CDS             complement(8324..9001)
                     /locus_tag="LZB08_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBM7313840.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(9139..9642)
                     /locus_tag="LZB08_000320"
     CDS             complement(9139..9642)
                     /locus_tag="LZB08_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7313841.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(9658..12321)
                     /gene="mgtA"
                     /locus_tag="LZB08_000321"
     CDS             complement(9658..12321)
                     /gene="mgtA"
                     /locus_tag="LZB08_000321"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBM7313842.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(12414..12623)
                     /locus_tag="LZB08_000322"
     CDS             complement(12414..12623)
                     /locus_tag="LZB08_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313843.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            12573..12908
                     /locus_tag="LZB08_000323"
     CDS             12573..12908
                     /locus_tag="LZB08_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313844.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(13107..14009)
                     /locus_tag="LZB08_000324"
     CDS             complement(13107..14009)
                     /locus_tag="LZB08_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7313845.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(14026..15033)
                     /locus_tag="LZB08_000325"
     CDS             complement(14026..15033)
                     /locus_tag="LZB08_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBM7313846.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(15030..17234)
                     /locus_tag="LZB08_000326"
     CDS             complement(15030..17234)
                     /locus_tag="LZB08_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7313847.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRSERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(17234..19684)
                     /locus_tag="LZB08_000327"
     CDS             complement(17234..19684)
                     /locus_tag="LZB08_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7313848.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(19662..20060)
                     /locus_tag="LZB08_000328"
     CDS             complement(19662..20060)
                     /locus_tag="LZB08_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7313849.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(20178..20681)
                     /locus_tag="LZB08_000329"
     CDS             complement(20178..20681)
                     /locus_tag="LZB08_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7313850.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(20699..21202)
                     /locus_tag="LZB08_000330"
     CDS             complement(20699..21202)
                     /locus_tag="LZB08_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7313851.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(21121..21417)
                     /locus_tag="LZB08_000331"
     CDS             complement(21121..21417)
                     /locus_tag="LZB08_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7313852.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSYQTIKDVRKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            complement(21500..22141)
                     /locus_tag="LZB08_000332"
     CDS             complement(21500..22141)
                     /locus_tag="LZB08_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBM7313853.1"
                     /translation="MEYKYTKIDLEKWNRGKLFQSYIDDMRIVMSLTVDIDVTNLIEF
                     SKSHDLKFYPAMIWVVSKVVNEHDEFKYSWDNKGNLIKWDYISPSYAEFHKEDENFTK
                     MVTDFSDSIFEFHERFMLDREKHKMERAFVANQPLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELERERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSIS"
     gene            complement(22349..22570)
                     /locus_tag="LZB08_000333"
     CDS             complement(22349..22570)
                     /locus_tag="LZB08_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313854.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(22571..22705)
                     /locus_tag="LZB08_000334"
     CDS             complement(22571..22705)
                     /locus_tag="LZB08_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBM7313855.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            complement(22718..23914)
                     /locus_tag="LZB08_000335"
     CDS             complement(22718..23914)
                     /locus_tag="LZB08_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBM7313856.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLNEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQATLSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(24098..25492)
                     /locus_tag="LZB08_000336"
     CDS             complement(24098..25492)
                     /locus_tag="LZB08_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7313857.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLEKGLTLSPYNLITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNSFYEGMVQRSEEMKQHSNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLKTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(25559..26173)
                     /locus_tag="LZB08_000337"
     CDS             complement(25559..26173)
                     /locus_tag="LZB08_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313858.1"
                     /translation="MSEKLLDLLKIFLEKYLVPTVIAIVFSFIAYYVTPTDNKLLVKF
                     TIWGYAVFLFCVWFLIIELVIWLVQKIQYHNYSKGVEQRNKQRQAEEWQQSLEFLWTD
                     VDNLSSSDYKLLLRFIKNENQPYCSSGTPMGHCLLNSDWVHKTVSKPAKRVLIKSNQR
                     TSSKAIPIPVYETISETYQYILREDIYQMLKYSYEKYGKISHFK"
     gene            complement(26264..26647)
                     /locus_tag="LZB08_000338"
     CDS             complement(26264..26647)
                     /locus_tag="LZB08_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7313859.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQTKK"
     gene            complement(26663..26989)
                     /locus_tag="LZB08_000339"
     CDS             complement(26663..26989)
                     /locus_tag="LZB08_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7313860.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(27228..30281)
                     /locus_tag="LZB08_000340"
     CDS             complement(27228..30281)
                     /locus_tag="LZB08_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBM7313861.1"
                     /translation="MKKMLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGETAYCIDINTDFKNGYKTRTDASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSISGATYGVFSDKDCTKQLATLTTDENGNTNVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSADLLPYGNFRIVESEAPNGYLTDGAKPIDFAITENEKIMDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKNGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELVIDGKRVENDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKIAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDITIIDTVT
                     LEGLEVGTQYKLVGWQMLKEKNAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEEEGQTITFKEKPEVPEEPEKPETPQTPE
                     EPSRPSDSPKTGDSTNVIAFLVMLLTSGGGLAGTYLYKRRKMKKS"
     gene            complement(30526..31890)
                     /gene="rlmD"
                     /locus_tag="LZB08_000341"
     CDS             complement(30526..31890)
                     /gene="rlmD"
                     /locus_tag="LZB08_000341"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBM7313862.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETCVLLQ
                     RRTM"
     gene            complement(32065..33825)
                     /gene="pyk"
                     /locus_tag="LZB08_000342"
     CDS             complement(32065..33825)
                     /gene="pyk"
                     /locus_tag="LZB08_000342"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBM7313863.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(33877..34836)
                     /gene="pfkA"
                     /locus_tag="LZB08_000343"
     CDS             complement(33877..34836)
                     /gene="pfkA"
                     /locus_tag="LZB08_000343"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBM7313864.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(35045..38614)
                     /locus_tag="LZB08_000344"
     CDS             complement(35045..38614)
                     /locus_tag="LZB08_000344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBM7313865.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNSIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(38771..39718)
                     /gene="whiA"
                     /locus_tag="LZB08_000345"
     CDS             complement(38771..39718)
                     /gene="whiA"
                     /locus_tag="LZB08_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBM7313866.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(39735..40160)
                     /locus_tag="LZB08_000346"
     CDS             complement(39735..40160)
                     /locus_tag="LZB08_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBM7313867.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(40229..41335)
                     /locus_tag="LZB08_000347"
     CDS             complement(40229..41335)
                     /locus_tag="LZB08_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBM7313868.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(41347..42204)
                     /gene="rapZ"
                     /locus_tag="LZB08_000348"
     CDS             complement(41347..42204)
                     /gene="rapZ"
                     /locus_tag="LZB08_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBM7313869.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(42235..42948)
                     /locus_tag="LZB08_000349"
     CDS             complement(42235..42948)
                     /locus_tag="LZB08_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBM7313870.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(42952..43866)
                     /gene="murB"
                     /locus_tag="LZB08_000350"
     CDS             complement(42952..43866)
                     /gene="murB"
                     /locus_tag="LZB08_000350"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBM7313871.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(44139..44909)
                     /locus_tag="LZB08_000351"
     CDS             complement(44139..44909)
                     /locus_tag="LZB08_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBM7313872.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        12991 a         9858 c         6935 g        15361 t
ORIGIN      
        1 attttatggt tttaaaaaaa ataattataa gtggatatgt gctttcacat gtttatcaac
       61 atttgtaatg cctatatttg ctaatgaatt ataaaaatat taaataatat tcaactatag
      121 attatgtaaa ttactctatg aaaaaaatga gttgtataaa atctggctac tgtagattga
      181 ctatttcttt taaaattaca attataataa gttttataga attaaaaata aaactttaga
      241 gatttttgat aacattgtag actaaatttg ccaccgaaat cgctttacag tggcaaatcg
      301 gtggcaaata caatatatta ttagtatata atctatataa acattactat tgtttgatta
      361 tcttagctac agtttccaca tgtgtcgtta ctactatcgg aacacatttt atcaacctcg
      421 ttgctgtcat gtgaacacaa ttcattagtc ctatgccgtc ctcttgcatt tttcctttgt
      481 gttttaaatt tagcaccatc tacgctattc ttcaagcctg ttttataaac aaaggtcaac
      541 tgtgcagggt caacatttcc gttttcatca agtccaccta caattacttt ttcaacaatg
      601 ctttcaaaca catagcggtc aaatttatca agaacctcat tctgttccag agttttctta
      661 aactctttca aacgtttttt tatgtctttc tcattatcag aagtttcttg taacttttgt
      721 ctttcttcaa caagctgttc ttgtttactt actaaatcag catatttact ttcgtaggtt
      781 tccttatcaa taatttcttc cagacgcata tccacaagtt tacttttttt cttctctaat
      841 gcgtctatct ccttttccgc ttttgcaagt tgttttgaca ccacactact actaagagta
      901 tcgtccattc tctgcaaaaa ttcttccaga acatctttat tatcatcaca taataaacga
      961 taactttcta caaatgcttc ttcaatagct gtttctggaa tacctttgct atgtttacaa
     1021 aatttctttc ctttcttcgt agccgttaca cattgccata taaccttgct gtattttgag
     1081 ccactgtgcc aattacgtct tgttaaagta ccaccacaaa agccacattc tagcatacag
     1141 ctaaatgcgt atttcctgct atacttctca cgcttaccgc tgcctaccgt tgttcggttc
     1201 ttatttctcc gattaagaat ttcctgtgct ttttcaaaaa cttcttcact aataatcggc
     1261 tcatggtgat ctttcatata aaatttatct tcttcaccca tgttttcaag gcgacgtttg
     1321 gaaatcggat caacagtaaa agttttgcct aagagtatat cacccttgta tttctcattc
     1381 ttaataattc caataaccgt agatggtgcc catgtggaac tcccatactt cgttttataa
     1441 cctagacttt caagctcctg tccaataaca gaaccaccag caccatcaac ataacggttg
     1501 aaaatatatc tgacaatctc tgcttcttct tgatttacag ttattgtctt atcctctggg
     1561 tgataatcat atccaataca accttgaaaa cctaccagct cgccgcgctt cattttcatt
     1621 ttcaaccctt tttttacatt ggctgaaatg ttctcgactt cctgctgtgc aacagaacta
     1681 agaacaacaa gcaataactc tccatccatt gtaagtgtat tgattttttc atcttcaaaa
     1741 taaacggcga tatttctctc ttttagcata cgaacatatt tcaatgtgtc taaagtattt
     1801 ctggcaaatc ttgatatgga ttttgtaaat accatgtcaa tttcaccatt catacagtca
     1861 tttatcatac gctgaaaatc ttctcgtttt gttacttgtg tccccgtaat tgcttcatca
     1921 gcataaatgt cagctaacac ccattccttg tttttcttaa tcatgtcagt ataatactga
     1981 acttgtgatt tataactgtt taactgatct tcgctatctg tacttacacg gcaatatgca
     2041 gctacacgca aacgctcaat ggcttttcca cgggcttgtc ttgccaaaac attttttgct
     2101 tttattactt caacttcctg catagtacac ctccttattt tgtgttcctt tcatattgtt
     2161 attgtactac tttaaaaaac acaattcaag cagttatgtc agaaagcacc ttatagtctt
     2221 tcatcagcct ttgttttaca agagtgtatt ctttttcaga aatcagttta cgggcaagca
     2281 gttgttttaa catagcaatt tgcatactat acctaattat attattcatt aaaaccctcc
     2341 ttgctttttt atttatggaa gtaatcagac ggcttcggca gctccactgg actttcacca
     2401 ttcccattct gatgggtgaa ccgtgtcata cgggtgtatc attattcaga tatacgagtc
     2461 atggcagcaa cctttccaaa gatcgcttgc ggttcactgg cataatcgct cgctgctttc
     2521 cccatttcag tggtcgtggc gttccagtcc gctggctcgt cgtatatcaa acgtatctga
     2581 ttactgtatt cacttgtcaa agaacggaaa agagttaaaa tgaggtggta gatactctct
     2641 gttatattta cttcactttg acttcaaacc ctaagattac tttaatcaac gctgcccgta
     2701 tcctgccttt cagttccata tcaagcacga tatacatatt cccgtgttcg tcatagaacg
     2761 ggcgtaggct ggcttttgat atgtaagcgt cataatggtt caaaatcttt tcaattgcca
     2821 cctcgttacc gtcagctgca gaactgatgg tcgagaatga tggacacttt tttgtagact
     2881 tcatcatgtt tttattcctc ctcgtacata tccttaataa gctttaatgt gtgcagcctg
     2941 tttctacata cggaaaatcg ttccatttcc ataacctctg caatttcagc gtcggtcatt
     3001 tcaagaaagt aggacataag cagaatgtta cgtcggcttt ctgttaattc tttgatagct
     3061 tcacataagc gttcatcata gatatggact tctgaaccaa atacatcaaa tgacgtaaac
     3121 tcaacgcaat attcgtccat ggcaccgatc tggtttaact ccatttcaga taaatcacag
     3181 aacggcgttt cacgtcttgc acgtctgcca agctctcggt catagttctt tacagttcgt
     3241 cctacaacct tgcgagctag acaatcaaat tgaagtctga tagcgccctc gaaagaagat
     3301 ggtttcataa tctcaccccc tttccagcga aagttgctaa agcgaaaaga cttctacctc
     3361 ttttcgcact aacactcacc atgaaaggtg ggatttgata cccaaccgca aaaatttttt
     3421 gaaaaagttt tatgcagtaa aaaagcacaa gggcaataca gacgtactga tcttgtgcta
     3481 aacaaattat tcctgttata tgctgtaaaa aaccacatat aggagcatta tgttccgtag
     3541 aatttaaatg tttttttaat gaactaccga tggtcgaaag ttgttgaata tgtaagtgtt
     3601 tcatacggct acccctagag ccgataaaac aagaccatcc agtaatttgt ccaacgttag
     3661 gcaaaaataa aacggcggct atataaccgt cgtcttattc aaaagttcaa aaagaactaa
     3721 actaaaccct ctaaagcagt atgtatttat aatcagtctt gcaaaataac aaagtagaat
     3781 tgaaatataa ggtagcaatc cttgtgcatc taataaactt ataggggtta cagcagacca
     3841 cggaataata ttttgtatac tttcagatca atatagtcca acaaaaccaa ttcccgatat
     3901 ttagtaacta aaaagtaaac tagagtagaa aacatttctt tgcaaaacac aaataataag
     3961 taatggactc attgacaata gattgagaaa tccagttttt tagacttaga tagattatct
     4021 cacttgtaaa tgatttaagt tctttgactt catatatagc ggtataatct gtcaacgtga
     4081 tcattacaaa ccataatgcc tatataacat actttccaag caaaaacttt attctattag
     4141 ttggggaata caaaatattt atcattgtgc gttcaacata ttctttttgt ataaaaatgt
     4201 aattacaaaa caccactcaa tataagatag ataataattt gaataggaat tatcaatcta
     4261 cgtaaccatt cattacctgt cattgttgtt acaatagatt tgggattctg tttccattac
     4321 ataatttttt tatccttctt tcagatatct cctcatttta tcgaagcttc ttctacagac
     4381 atacattcca taataaaatc tacctttgaa tattatttca cgatttcgtt atagacgcgt
     4441 aaacaaacaa taaaaaacga aatttctttt aagtaccata atttttatat cgaaattaca
     4501 attatccact tatatacagc atttataggt aattcaatga taaaaaaaca catactttac
     4561 tactaaattg ccacaattga atgcgccttg atatgatagg gagattccac aataaaaaac
     4621 tgtgcatctc ccttgattca ttcatactga tatcttattt ataagactct tttgtgtctt
     4681 tttttggttt catggaccaa aatactacat tcattacaat aacaatcagc aacacaactg
     4741 ccatagccac ccaaaagccg atattaagac caagccattc ggtagttcca aaaagggtca
     4801 tccaaagttc tttccagttc atcattccac ctccttaaag cagttctccg aaatgacgga
     4861 cataggcttt ttttaaactt gttgccaaag tcatatacag cagaatacat ggaatcagat
     4921 aagcaaagta tgctgcaggc aatgcaacaa atccaagcat agttccaaac acagtaaacg
     4981 gaataatggt cagaactata attccagtca ttgttagcaa agtcacagga gctgatgcac
     5041 gactctgaac aaaaggtagc tttggagtac gaatcatgtg aatgactaat gtctggctcc
     5101 acatagattc cacgaaccac cctgcttgga acatcccgat atactgtgcc tgaagcattg
     5161 ccagttctgc accgctgaaa tgagcaggca aatcattaaa taatacacca ttcgatacga
     5221 acaatggaca aaatacaaag tacataaaaa tataggttgt aaaatcaaag atagaactgg
     5281 ttggtccaat ccagatcata aaacttccaa cactggaagc gtcccattta cgaggtttgg
     5341 taataaactc ctcatccaca ttatcccaag gaattgcagt acaggataaa tcataaatta
     5401 agtttagaaa tataagatga acactcatca ttggtaagaa aggtagcaaa gcagatgcag
     5461 caagcactga gaacatattg ccgaagttgg atgaagcagt cattttaatg tacttgatca
     5521 tgttggcata tgtctttcgt ccctcaataa taccctgttc caaaaccatc agatcttttt
     5581 ccaatagaat aatgtcagca gattcttttg ccacgtccac ggcggtatcc accgaaatac
     5641 caatatcggc ggctttcata gctgcagcat cattaatacc gtcacccatg aaaccgaccg
     5701 tatgaccatt ttcacggaga acagaaacca cacgggcttt ctgatcaggg gttagctttg
     5761 caaatacatc tgtagactct gccactcttg caagctctgc atcgcccatt tgttccaaat
     5821 cagaaccgag aagcatattg cgaacctcta atcccacctg cttgcagata gttcgagtca
     5881 ctttgtcgtt gtcacctgtc aaaattttgg ttataacacc atggtctttc agagctttaa
     5941 tagcatccgc agtagattct tttggaggat caaggaatgc cagatatcca atcagtacca
     6001 tatcacactc atctttaaca ccaaatgcgc cgattgggga aggattactc ttctgtgcaa
     6061 tagctagtac gcggaatccc ttatcgttaa gattgtccac tgtctccaga attctgtaac
     6121 gaacgtcatc agtcagaggc tgcactcctc cattacactc tgcaaacgca caaatagaga
     6181 gcatttcttc cacagcacct tttgttacca tctgggtctt acccttatta tcctgtacca
     6241 ctgtagtcag acgacgacgt gtgaagtcaa aaggaatctc atctactttc acatagtttt
     6301 cagacaggtc aatgagacgc gaatctgcgg cttcctcttc ctccgtctta tggataattg
     6361 ccaaatccat caggttttta tagccagtct ggaagtaact attgaggtaa gcgtgacgca
     6421 gtactcgcgt atcgtcctcg ccatttacat tcagatggta ttccagcaca accttatcct
     6481 gggtcagcgt gccagtcttg tcagtacaga gaatatcaat agcaccgaag ttctgaatgg
     6541 aattgaggtt tttaacgatt gtctgcttct tgctcatgga aacagcgcct ttcgcaagac
     6601 aggttgttac aatcatcggc aacatttctg gtgtcaaacc aacagcaata gaaacgccga
     6661 ataagaaggc cgccaaccaa tcgcccttgg tgataccatt gacaaagaac accagcggaa
     6721 ccataatgag cataaaacga atcaataccc aagaaacggc attgacaccc ttggtaaagc
     6781 ttgtttcaac cgcttctcca gcaactgcgg acgccataga gccaaacaga gtatgatcgc
     6841 caacacaaac aacaacagct gttgcgctac cagaaattac attactcccc ataaaagcaa
     6901 tgtttgtgta atcagtcacg ctttctttct gagcacttac attgggtgtt ttttcaatag
     6961 gttcactctc accagtaaga cttgcctgac tgacaaacaa atccttcgca tccaaaatac
     7021 gaacatccgc cggaatcata tctccagcag aaagatgcac aatatctccc accaccaaat
     7081 catccatagg aagttctttt ttttcatagt ttctacgagt aactgtacaa gtggtcgtaa
     7141 tcatggctaa caatttctct gctgcatttc cgcttctgga ttcctgtaca aaacgaagcg
     7201 taccggaaat gaataccatt gttaaaatga ttacaacagt cagggaatca aagtcctctg
     7261 gcacactgcc aaacagagaa aaatacggaa aaatcatatc tgtaattgtg gatactaaag
     7321 ccagacaaaa taaaatggcg gtaaatggat tgataaatgc gccaaccagt cgttttgcca
     7381 gagatttctt tttttcatga gtgactttat tggtaccata tttagcacga cttacggata
     7441 cttcatctac ttctaagcca cgaagtgtgg tatgaagttt ttttaaaact tctttaatag
     7501 gattggttgc cgcaaattga atgcggcggt tctgttcgtc acggatgact gctttttcta
     7561 cagtctgacg aatagccata cgattttctt ttttgttcat tggtcttacc tccttgaatt
     7621 ttcatagggg caaggaatat tatatgagcg acacggaatc tccaaattct catataatat
     7681 tctctgcctt cgtggactga catcagagtc catgtctttc acctcacaat cttaaaaaaa
     7741 tatctatgtg aaacctcaat gtggattatt ggttgcgata gcggacactt ttttcgatat
     7801 accagacatc aaagccggca acaatcagcc atgcgattat tgcaaataca aaccagtggt
     7861 ttgaaaatcc cggcaaaaat cctttcatta agaatataat cccatttaac gcaatcattt
     7921 ctccaacatt acgacaaagc gggacaatat taattttatc tttctcctcc tgcggcatat
     7981 ttttccacag ggaaagatga cgatatcctt ttccaaaggc caaccaaaag cctgcaagag
     8041 taaataaaat tccaaaaaag atacatgtaa aatccatcgt ttgttcctcc tttcaatgct
     8101 taaacctgtt tcttagtttt gtcagcttca gaagtttaat gaagagcaca ataatagtaa
     8161 cagcaagaat aaaccagatc acaatactca cctgatacca tatcttaaag attaccattc
     8221 tttgttcaaa gaaattccaa cccaaatagc tacataagat tgtaaataca gggtagaaag
     8281 caaaaagaat tgctcgtttc aaatcttcga tttctctcct cactcacaac acctcccaac
     8341 cagcactaat cacttccgga attaccaata cctgcccaac gattccttct aaaatattgt
     8401 ttttggattt tccgacagaa cagtattctg cgacaatctc aaccttgtca ccaaccacat
     8461 caccgctttc cagattgttc aaaaagagcg tcttacagga attgctgtta atcagcaata
     8521 agcgtatatc ctgctctgcc ttttcctgac atgtaaccga aatacgatac tgcttttcag
     8581 attcatcact ggaagtaatt gggtgaaact ttcttgcaaa gggacgtaga atcaggttgg
     8641 aaccaattaa aatagtagcg gcggtgatcg ccattgcata cattcccgta cttgcaagaa
     8701 ttccaatcgc tgcagtacac cataaggtgg cagcggtatt cattccacgt actgtccctc
     8761 tgtccttgaa aataacacca ctgcataaaa aaccaacacc ggatataata ctgcttccga
     8821 cacggaatac ctgatccgaa ccgtagagca taggaaacaa ggtaaagaaa cttgtaccca
     8881 tgcagattaa cacattgata cgaatacctg caggatgacc agtcaattgc cgttctaatc
     8941 caataagaaa tccaagtgtt aaggagaggg caattctcaa aataaaatct gtgtaaaaca
     9001 tatgaaacac ctccctttaa ctactaacag tatataaaaa atccaaatac aatcctatgg
     9061 ctaaagtctt gcgattttct tgcgatttgg taagaaaata gacggactga aaccgaaaat
     9121 ggtcttagtc cgtctttgtc atatctgatc attaaatttg tatccaatac cccagatggt
     9181 caaaatatat gtgggacaat ccggagcagg ttcaatcttt tttcgcaact tccggataaa
     9241 agccataatg ttgctgttat ccagaatata atcttcctcc catacggcac gataaatctg
     9301 ttcctttgta aaaacttcac ctttgttctg gacaagaaag tacaaaatat caaattcctt
     9361 tggagtcagg ttgacttcag aaccttttat cagtactttt ctgcctcttg gataaatttc
     9421 cagatcccct gccaggattc tatctgacag agtacccact ggccaatcgc tttcttctga
     9481 tgctcctaac aataacgaag atacctctcc acgaatcatc tcctgaatac cagaaagtaa
     9541 atccttcgca caatcactgt ccggtggatc tcttagtagt ttttctaata gccagatttc
     9601 aagagccgaa tccatcggaa tataactaat attctttttc atcatgaatg cctcctctta
     9661 aatcaactct tgatattttt tcttataaaa atatttggct actgaattca gcagcatata
     9721 gagagatacc accagcagta aaaatacgaa ataccaaaga ggtaatttcg tcagtccaaa
     9781 caacgacgca ccgccgacaa atgtaaggga agtaaaagca ataatcccca ctagagttat
     9841 agaaatgacc ggtgcagatg ccctgctctg gaaaaaagga atcttggaag ttcgcagaaa
     9901 atgtaaaatc aaaacctgtg tccacataga ttccaaaaac catccagtct gaaacagtgc
     9961 tacatactga agttgcaggg aaggatctgt aagctgcaga taggttgccc ctccacatag
    10021 catcggacat aggaaataat acaaaaatag gaaggtgaca atatcaaaca gagaactgat
    10081 cggtccgaaa gacagcataa actgtctcag tgttttgccg gaccagtctc ttggggacag
    10141 catctcttcc tcatctacat tgtcccaagg aagcacaata cacaacgtat catacagcag
    10201 attcaaaagc agaatctgaa ttgaagtcat aggcaggaac ggtaaaaagg cactggcaca
    10261 aatgatggaa aaaatatttc caaagttaga gctagcggtg attttaatat atttcagcat
    10321 attggtaaaa gtttttcgcc cctccattac accttgctcc aaaacattca agtccttttg
    10381 cagcaaaacc acatcggcag catcttttgc tgcgtccact gccgtatcta cagagattcc
    10441 cacatttgct tcattcagtg ccggaatgtc gttgactcca tcaccaagaa atcccacgga
    10501 atgaccatta tcctgcatag cagagacaag acgcacctta tgtcccggag taagctctgc
    10561 aaaaacatga gtatgttcca cagccttttt aagttcacag tctgtcattt catccagttg
    10621 agtaccagtt aagatacgct ctgtataaat acccacccgg cggcaaacag acacagcaat
    10681 cgctgcctgg tcccctgtca gaatcttagg gataactttt aatcttttga gtgcagttac
    10741 agactccttt gctgtctgct ttggtgcatc aaagaaggat agatagccaa ctagagtcat
    10801 gccccattcg tcatcaggag ttattttctt ttgcgctccc acattttttc gtgccaccgc
    10861 aatgaccttc attccgtcct gtaacatttc acctactaca gaagatacac tctgtctcgc
    10921 atcctgcccc atggggagaa gttttccacg atattctaca tggttgcagc gtgagaatat
    10981 atgttcaata tcacctttca taataagctg actattccca ttgctatcct gtacaagtgt
    11041 actgacaaac ttacgagcat agtcaaaggg aatttcatca gctttttgga attttgtcag
    11101 aagatttgcg taatgggctt cccgccctgg catggtattg caggcaagaa tggcattatc
    11161 aatggtattg cagacaccag aatgatagca gctattcaga taagcaagat caagaacctc
    11221 tgcattttca tttccaaaaa tatccatata atattccagc aggatgcttt cattggtaag
    11281 tgttcctgtt ttatccatac aaagcacatc catgctacca aagctctgca tggcattaat
    11341 atccttgata atggtctgct ttttgctcat agcaagactt cctttggcaa ggcaggctgt
    11401 aataaccata ggaagcattt ccggcatcag acctacagca actgacaggg cgaaagcaaa
    11461 agattccatc cattttccat cagtaatccc cagtaatata aatacaatgg gaactaatat
    11521 cgccataaaa cgtatcatca cccaggcgat ggaatcggct ccttttttaa aggaattttt
    11581 atcatcagaa tccggtttgg taaatcctcc atacagggta tctttgccta ctgccagtac
    11641 aatgccttcc ccttttccac tgatgactgt ggttgccata aaagcgaggt tttcaagctg
    11701 agtcagtgta tcctgctctc cgtaactgag ttttcggcag ctcttttcaa tgatggcgct
    11761 ctcgccagta atcgcagcct gagagataaa gagatccgta atcttcgtca atctgatatc
    11821 cgccggaaca cgatcacctg cagagaatag tacaatatcc cctaccacta attcttcgcc
    11881 aggaatctct attaactttc catcacgcct cactgtgacg ctttcatgaa ctaggcgatc
    11941 aagctgagca gcagcgctct ttgctcgcag ctcctgaatc agacgaatta cgccactaat
    12001 tacgatcatg gaaaagatga taatggcggt agtagcattt ctcgcaaaat tggatgctac
    12061 gaaaacatcc gtaacaagag aaacaatgcc cagaacaaaa agaatcacat gaaacgggtt
    12121 aataaatgcc cgtctcaacc gttgcatcgt cgtatcattt ttccgcccat tgaagctgtt
    12181 cgctccgtat ttctcccgca ttgaaccgat ttgctcaatg gataaaccat ccggagatga
    12241 gccaatgtct cgataaattt ctgatgttct acagtatgcg tattttttga tacgactatc
    12301 aaatagtgtt gcttttgaca cgaaacatcc cctcctttat aagtataagt aataatattt
    12361 taacacagtg ttctgaaaat gctatattta tattcttgct tttttcttgc aatttacttt
    12421 gatatggcat ttttcagctc gatcaaatcc gtctcgttag ggcgagataa atccttatca
    12481 aagttttcaa ccattccttc taaatttgga gcacggatgg atacggcaag cattttctca
    12541 tagcgctctc gcacataaat aggcatttcc ccttgacaca tatgagtgct gacaaacttc
    12601 ttcttggttt tcaccttgac catgtatcaa atttaaatga actacttaga aaagttgctc
    12661 ataataatat ttgtattatg agtgatagaa tgacaaaagt ggcagtatgg aaaccgagtg
    12721 taaaaacaat acaattagtt tgtatggaat atctgaacta cataattgac cactctgtaa
    12781 tttatgaaaa aatacaatgc gcaaattgga acgggacaga agaaaccaat agtattttta
    12841 ataattcccc ttcaccattg attactctta tctattcttg cgatttacaa caaaataaaa
    12901 caacataaat tttaaatacg ttgactggca taattcatat gtatagtaag tctacaatta
    12961 agatgcgctt tttctaatcc aaatatatta agaaaaaaat tttgcgaagc cattaatact
    13021 ttgtttaaga tgtctttcat aaatataaag aataatgctc ttaacaaaag cgtgcaaatt
    13081 cctaaagaaa tgcacgcttt caataattat cctataatct tccaattgct ctccttatgt
    13141 agcactaatt caaactgcga tacctgtgtt gcctttgtct gattatcaat gaatttcacg
    13201 gcaaccttga ctttcacatt gtccccatca accgtaaaga tagggtttac caattcagaa
    13261 tagaggtagt ccctgccgat agactcaagc acatttccag acacatagta agcaagctcc
    13321 ttttctgtgg ctgttgggta tagcttaaag aaagtttcca gaaatgcagt agcgtcattt
    13381 atggtatccg cgtctacact tgcgtctgct tctggtgtct ttggttcata gtctgatttc
    13441 tcgattgctg gtgcaagggt agggttctga ataattacca tattcccgcc agcgtccaca
    13501 tgaactttta cggtataggt tgccttaacc gctgttgtct gttccccctc ttttatctgc
    13561 tgatctactt cgtaggtagc ggaaaaagtg tccgttccag actgttcaat gctccataca
    13621 atcacatctg taatggcaga gctggtcggt atatccgttc taatggtatc catattcaag
    13681 tcctgcaatt cctttgtcag ataaccgctg attgcctgcg tccttgcgtc aatcgcttct
    13741 ttgctgttac tccatgtgta ataggacttt gcaaaattct tcacgaaatt ttctatcccg
    13801 ttggtgtcct gcaagcgaag ctcaatcact tctttttcat gggtcgtgtg ctggtcaata
    13861 gcggtaaaat tcttatatac tccaaagctc acgcttgcaa taagcaccac ccacaatgca
    13921 atcacggttt tcttatgtgt gcctaccttg acagtacgta cctttttttc tttgacattt
    13981 tctgtctgtt tcttattctt cttaaacata tttttcaagt cctttctatt gttttactcg
    14041 tcctgcaccg attaagtgct gttgccagta actactactt aggtctgtat atcctatcgg
    14101 gtcgcctgca tggtacatct gattatttcc cacatagata ccgacgtggg ttacatacga
    14161 accagcgtta taggtggaat ggaaaaatac caaatcgcca gcctttgcct gcgagagtgg
    14221 aagatgttgg gtagcgtcat actgggcttg tgccgttctc ggtaaggaga taccagcttt
    14281 tccatagcac cattgcgtca gtcccgaaca gtcaaaggaa gtggttggag aagaaccacc
    14341 aaacacatat ttccaccctt gatatttcaa cgcttcattc attacctttt gtgccagctc
    14401 tcccgatacc tgcggaacag ttaaatactg attgactaat tccacataga acatatttcc
    14461 atagccatac cgccagcctc cgttcttcgc aacggctatc gggttggtat aggttacttt
    14521 ctttccaccc gatttctcac gggcgaagct ctctgcaaga ttaaaagtgt gtttctttcc
    14581 gtttcctgcc acatagccca catagccacc gccatagtta taggactgta tcgctacatt
    14641 caaatcgtcg atatcttgat ttttgcagga agaaagcagg gacgcaaaat acttacaccc
    14701 ctgcttgatt gagctttccg tatctaagga actaggcggt aaaccaagac tttctgaact
    14761 ctgcataaca tcttctgccg taccgccact ttctacttga atgatagcca gtagtacatt
    14821 gacgtactcg gagataccgt tttctctggc gtatttttct accataggct gatgtttcaa
    14881 aacttctgcg gataagttca tacccgtaat gccagaagaa aagttgctgt tttcgtcgtc
    14941 gctgtccgca ctaatcagca ccccaaagaa aagcaccaga gaaaagagaa taggaaacag
    15001 acttccaatg atagcgatat gtttcagttt cattttttct tctgtccttt cttcgttaca
    15061 agattacgga ttttctctgt ggtctgctgg ttcttctgga cagtctgggt ctgctgaacc
    15121 tttgtcctac ggtctttggt gtaattctgg cgtgtttcct ttgacacggt aactttttct
    15181 acattctgcc tatatgtcga ttgggtcgct ttcccgtcag gagtactaga agatagcgga
    15241 cgttctttga caccgcttgt cttaactggt tcgcttgctt tcgtagtcgg agttgtggca
    15301 gggcgtttga cttcctgcat tttttccgca cccgtctttg aaacggttga agctgtggct
    15361 ggtcgctcat ggggacgggt agctcccgtt gtcgctgatc cgtcagcctt tcgctgtgcc
    15421 tgccttgctt cttgtgcctt ttgaagctcc atacgcttga cagcgatact tttcttatgc
    15481 tgttcctgct tttccaagcg tcccgtctgt ctggactgct gttcctgcac cataccacgc
    15541 ttgaaatcag acacgctgga ctttgccttt tcctttgcgg aatacaccgc ataagcggtc
    15601 tgtgtgggca tatccttgat attctctttg acagcgtttg ccttgtcttt cactttattt
    15661 ttcgtatcta agacagcacc cacctttgag cctgcacgct gtcccatact ggaagtggta
    15721 ttgccccgat tttctttaga agccgtattt tttctttcgg atctcttgcc agcaacggca
    15781 cttccagcta ccgcaccagc cacaccgccc gaaataccgc cagcacttac agcccttgca
    15841 atacggtgtt ccatacgcct agcccgatgt cgcatgaaca gatacggtct gcggaatatc
    15901 ctgcgtccca tgctttgact gtcgccagcg ttgagagaaa acatactcat taagtcgccc
    15961 agcttcatat agataccagc gaaacatact atctgcaaga acgccaccat gaaaaacgga
    16021 tagtccgtgg atatgttata aaacatactg gaaatgctaa acgcaaccgt tacaatgagc
    16081 gttattcctg cccgtgtcat tatggtatta aataccctca cgattgcctg ctttgccatg
    16141 ctttcatagc tcggtatcat ggaaagtaga aagctgatag gtaaaaacat agcgaagata
    16201 ataaaaagta tctggctgaa caacatcata cccgtaagca agaatacaaa tatcgtaata
    16261 ccaagattaa agaacaagag gaagaacacc atacctaaac ggtttaccac ctgcggtatc
    16321 gtcagattgt tgttgtcgtt gtcctcgatt tctgttttca cgacttcttc tctggtcttt
    16381 ccgtcctcgt cctctggact tgccgatacg agagcttcga cacggtctgt cccgatttcc
    16441 tctgcgttgc tgtttccaaa ctgtaagagt agccacggct gttgtacttg aatagaaaat
    16501 aggctgtccc gtatcaagtc tacgctgtcc ttgccctcgc tgtcagagtt ggggagcatg
    16561 atttttgttc ccaaatcaag cgaagcggta ctgatgtctg atgaaaattc atttatcttc
    16621 ttgatgtagt cgggagcgta ggcaataaac gaagcggaca acacgaacac caccacaaag
    16681 ttgataacag cgtgaagtgc cttgctggtt tcccgtttta tcagtcccgt ataggcaaca
    16741 tacattccca ccactaagat aataagaagc aggaaaccaa catagaagcc cgtagaagaa
    16801 aagccgttct cggtaacgcc tgcaagggtc tgtatgcttt ttccgatact gtctgccata
    16861 tcgttaataa aatcaagttt ataggcttcc tgcaccacat agccagttgc attgcttaaa
    16921 taaaggctta tcgtccagac aaagttggta atgcagtaaa gcccgtactg taccgatttt
    16981 ccgataccgt ccagccagtt ccacggaagc cacgaccagc tattatcgac gaaaaaatca
    17041 agctggtagt tggaaagcgg atattttgaa tatagatttt ctgcgtttat ggtatcgtcc
    17101 acaagccccg tcgcatgagc caccgtcccc agaagtgaaa gcaggataag ggaaagtgcc
    17161 accacgaaca gagccatttt gagaaagtga agtatcttcc tttttgtgaa cgcactctgt
    17221 atcctttctt tcatcactcc acctcatttc tctgtacggg cggtctagta tcaaaggcgt
    17281 gtagtaattc ttcaaagact gggtgtatct gcaccacacc gacacgcccg tataagtcct
    17341 gcaataaaca ttgtccgttc tccaaatcac gcaagcgttt ctggttgttt tcgtcgtcct
    17401 tgtcgatacc gaaaaattct aaggtctgct ttatctcgtt gatgtcagta gaacgaaatg
    17461 caaacttcaa accgatattg tttttcagac tttcctttga cacgtcatag gcagactggg
    17521 taacgaaata aacgcctgcc tgcatagctc gtccagcacg aaccagctta tttgataggg
    17581 tttcgccttg tgccacattt aagaacgccc acgcttcgtc caaatctaca atcttaaaaa
    17641 tgcttctgtc cgaatggata aaatcaaggg caaaggtact aatcacaatc agcatagaca
    17701 ccgacaataa ttcaatggtc gtgtattcct caaaagtggt atctttatct ggcagtacaa
    17761 ggtctgctac ttgaatgata ttgagctggt tatcaaggct tatagcattt tctaccgtcc
    17821 cgtctgaaaa cagcaggtgt gcaaagtcgt agtccgtgaa gctgtcgata tggtctgcga
    17881 tattgcgtga gatagcggta tcttcttttc tcaattcctc aataacgtgt aacagtcctc
    17941 tattttcact ctgggtaaca gaacgcaccg cttttcgtaa cacggggaat ttttcgccgt
    18001 ccctagagga aatacccgta aggaatgtta agatgtcgat tgccagactt tcagcgtctt
    18061 tcacattctt cataatcaca aatgggtcaa gaagtcctgc attgtccttg tcgctggtaa
    18121 ggtttacgat attgatttca tgggctatct ctgggagcgt ttctttccag ttgccacgct
    18181 cacttttagg gtctaacaga agtgcttgac cgccaaacag caccgcataa tatacaagta
    18241 gattgttgca gaatgacttt ccaccgccaa gacttcccac aaaagcagaa gcaagggcat
    18301 tagtaactgt gccttttact ccctgcgaag ctaaagacgg ttgcaggtac acatttcttc
    18361 ccgtatcaac ggaatagccc atatagatac ccgtgttttc ccctaactgc tgggtcgcac
    18421 caaaaccaag cccagccaag aagtctgact ttacatactg cacatagtca ttgatatatc
    18481 gcttgctggc aggtaaaaat tcagaatgaa gtcccaacat atcgccagca ggacgcacca
    18541 gttttacatt gaggtcgtcg taaaagtcct tgacttcatc acaacggcgt ttcagctcgt
    18601 caagatcggg tgcagacacc cgtatgacgt agcttaactt atacatactt tctttgctct
    18661 ggtctaaatc ggtttccagc tcgtccacgc tgtctaaagc gtccaccaca tttgagctgg
    18721 tttcacttcc tgcttgatag gcgtgattat ccaaatcttt caattccttt ttcttgttgc
    18781 ggactgtcgt taatgcctta cggttctcca cgatttctac attcatagaa gtatcaacgg
    18841 ggaatgtgaa ttgctgttgc tggaaataga agatttcaga ggacggaaaa tccaactccc
    18901 cgacaattac attgacggta aaataggaca catagctttc cttgtcctca tgttccaatc
    18961 gtaaataccg ctggctttcc tcaatcacac accttgtcgg acggataagg tcgtagtatt
    19021 ttatcagcgt ttctttcttc aatttcttct ttggtagctg gtactcataa tcttcatagg
    19081 cgataccgtc cctgccgtaa agatgttcca tgagataccc aaaatcatgg atttccaagc
    19141 gacgcacctt aaagcgacgg gagattttat tttccagtaa cttttccatt ttcatgtaac
    19201 ggttgatttc atcattcggc atggaaacaa aatcattcat cagcgtgtgg ttcacttcat
    19261 gaagaaattc cttgaacgtc agccatgccg attttttgat gttcttcaga ttgagctgtt
    19321 cttccgtaac catgagctta aagccaagaa aaaagcggta gtccacttga ttgtccccaa
    19381 tcattgatac taacgcttcg gtctgttcgt ctattttttg gactgccacc tcacgaagtt
    19441 tgcctgttac cagtttcttt gactgctcct gcatacttct aatggagctt tctgtggcaa
    19501 tctgcaacgc atgaattttt ccctcacggg actgtgcgat tagctgtcga aaactgtcat
    19561 gcacgataaa tttctgctct gcggatagaa aagaatagtt gtacggtatc agctcatagt
    19621 aggcgaacac ctcattgtcc ttgttccaga caaggttatt gtcaatatat tttatcggga
    19681 acatagttca cactccttac tgccgtgatt gtttcattca atatctgctt atgcagtttt
    19741 acggcttttc ctgcataagt gattttaggt cgcaaggcat aggttatctg tgatttcaaa
    19801 aagctgtacg gcttctttcc gtcaaaggtt ttctgcgaca taaaccaagt gagagcaacg
    19861 ggaataccga agtatttgag aaatgctccc tcaatcatgg aaagtggggg catatcccca
    19921 aacagaatga tgataaattc cgtaatcaca aaccatgtaa tctgtgtaaa ggtaacggga
    19981 aagggtaagt taaagtcatt gattgcatat aagacttttt ccacgttcca gatacccgtg
    20041 tagcttttaa tctttttcaa gttctttcag cccctttcgt aagttcttca aagagaaaag
    20101 gacagccatt ttcagactgt cccttaaaga gaaatacgct gtcagtagca acaatgcttg
    20161 ttgctgatct tccagcgtta atatggtatc tcacagatac ctcggcttgt ttcaatgaat
    20221 cgaccaccca tatccaaatc tcgcccgtag gcttcatagt caatatagtt ctgtaagtta
    20281 gctggaatgt ccccaagtgc gttcaattcg tctatgtagt agtaggcaac atctgtcatg
    20341 gtttcacaat cgggataaaa ataaatatcg tccttatgtt ccacgacttc ttccagcgtc
    20401 ccatagtggc tgataaattc gtccagacac tctacgatat agtcgggaag ttcctctatc
    20461 atttcataca tttcattgag ttcttcaatg gaaacatatt cccctatgtc gataggaaag
    20521 ttgtcggtat catggacagc gtattcttcg tactgttcat tcaagccgat tttttctttt
    20581 acatcttcct cgtcaatagg gaaagtgaac cacgcaccga ctaaatatcc ctcattgtat
    20641 ctgccaagat tagcaatata aaccgccata tcatcaatca taagcaccac ctcatttcct
    20701 actctggcag ttcaaagata ccgtgttccg ttactaaaaa ttttccgtgt tcctgcaagt
    20761 gagaagcata ggcttcaaag tcaaaatagt attcttgaca gtcctcggat aaatgtttga
    20821 atgtcgggtc gttcatcagc ttttgccttg caacatcaat catgcttttg cagtcgggat
    20881 aaacggtaat cacgttcctg caaatataaa gtgcttctaa attctcatac acggtaagta
    20941 gtgatacata ttcttcctgc atatcacttg aaagctggcg gtacataaaa tctaattcgt
    21001 tgagctgata cacgctggtg tgttcgtgga cttcatcagc ataaggcagt accttttcga
    21061 taatgcggta atccccactt tctgcaccga cacctaattt ttcttcaaac tcggcaacat
    21121 ctatcggcag gtcaaaccag tatgatattg tttcttcgcc agttgttcct ctcgtttcca
    21181 ccagcaccct tgtttcctgc attgttcctc acgtcctttc tgttgtttcc tcacatcttt
    21241 tatggtctgg tacgacatac caaacacata ctttgaaaaa tcttctaact gtttttcttc
    21301 ataatcggca gggttcagtc gtttcatttc atgccacact ttacgcttgt aagagggcag
    21361 gtcggaaatg tattcacagc cgacttttgc ctgcatatcc ttaaaaatat cgttcatttc
    21421 atcactccaa tctgaaattt tggcaagaaa aaagcagtca atcctaagac taactgcttt
    21481 gtgtatatct ggtattagat tatgaaattg agtttatcaa ttcttgtact tcattaaaaa
    21541 atctggataa atgaaaacca tctgcaacgg catgatgtat attcattgta agaggcatta
    21601 tatatctttc tcgctctaat tcgtatttac cccaagtaat aactggtgca agaaatttcc
    21661 cttcgtcaaa aacatgaaca tcaaaatgat tgtattttac ccatggcaaa caagatacat
    21721 caaaaaagtt caaaggttga tttgctacga atgcacgttc cattttatgt ttctctctat
    21781 ctaacataaa tcgttcatga aattcaaata tgctatcaga aaaatctgta accatttttg
    21841 tgaaattctc atcttcttta tgaaattcag cataggacgg ggaaatatag tcccatttaa
    21901 ttaaattacc tttattgtcc caactatatt taaactcatc atgttcatta acgacctttg
    21961 aaacaaccca tatcatagct ggataaaatt tcaaatcatg gctttttgaa aattcaatta
    22021 gatttgttac atcaatatct acggttaagc tcataacgat acgcatatca tcaatgtaac
    22081 tttgaaacaa tttacctcta ttccattttt ctaaatcaat ttttgtatat ttatattcca
    22141 tgtccattct cctttacatt ttgaaattca gtaaaatgca aggctctctg gataacattt
    22201 cactccatgc aagtctattc cattaagcct tgtatggaga taatacaata cacaatttca
    22261 taatatccct ccttatccaa taaaccttag tttattgttt tcatactcag attataccat
    22321 tcatatatag gctctacaat aaaaatcatt atgcaccaat aatcttattg aacagctcta
    22381 acagtacatc tttcacaccg ccagcgttga atactaagcc gacagcaata agggcaacta
    22441 ccaagaagcc aataagtttt gaaaactcac gcttgaaccc taagtagata ccgataacca
    22501 caatcgccat aagcactaag ctctgtgcat ttgataaaaa ccattgatac aaattttgtc
    22561 cgaaattcat ttaattgtcc tcgctttctt ctaattgttt catttcatag tcagcttctt
    22621 tgccgatact caaaatacac atcaagacta cgccgatacc cattcccata gataccagca
    22681 ggaagtcttt tactaattcc cacatttttt atcactccta actttccacc aaatcttttg
    22741 caggggtcgt ctgctgtttg attatcattt catgcttttc actcaaagtt gcctgctttt
    22801 caatcgtttc catatagtca gtcccgtttc ccttatcaat ctttttcagc attttcaagg
    22861 taggtgctac ctgcctttgt acccaccgca atgtacggtc taaggtgtaa ggctctggct
    22921 ttgtcgtcag cttcaagctc tgtctgttat cgccgataaa ccaagcccag cggtcattga
    22981 gtttccagtc attttttcgc ttgtctggtt cttcatcaac aaaccgcaca tactggttga
    23041 tgatagaaaa ggcggtctgt tctgcgtcat aataggtcag caaatctcgt actgcataat
    23101 aggcacgctc atttctaagc cgtatctcaa aacggttgat aatgtcggct tcattaagcg
    23161 gtgtacccaa cttgacgtac tgctcatagt ccttttcata gatacagaaa tatacatctg
    23221 atttcagcga accaagataa agagtgcgtc ccatatattc tctgtcgtcc tctctgtgct
    23281 tgataagctc gcccgactgg taaaacttat aacttctgga ctttccgata tattcccgtt
    23341 tcctgcattt ttccgcaagc tctggaatat ccaaaatgcc cgtatggtcg ttgatagcaa
    23401 ggtcgatacg cttcatcacg ccaccgtcta cgagtgcgtc catgaggaag tcataccagc
    23461 ttctttgctg tgccaacagg taactttcaa actgcctgca accacgcccc tttaactcta
    23521 aaaggacacc tttttcttcg tcagccgacg tatagataaa gatgtcccct aaagagtaat
    23581 gctccgtata gctgtaatgt ccgtaatctt catggagcat atagttgata ttcagtttta
    23641 atatatcttt gatgatgtgc tgtatatcca gcgtgggaaa acgaattttt acataatcaa
    23701 acaacatggt aagcggtgct tctggattga accttgccag ttccttatgc agagtttcca
    23761 gaagttcctt tgacggcttc atttttccgc tttctatctt attgagatat tcccttgtta
    23821 taccagaagc caccgccagc ctgccttgtg agataccgta agcaatccgt ttctcccgta
    23881 attcttttat ccattgttct tcattcagaa ccataccccc aatctgtaaa gtgtgaagtt
    23941 tttaggggcg acttcacaac cgatttttgc taggaaacca tgccagaagc cttatgtttc
    24001 ctatgttttt tgtctattgt ctatttgcaa acgtacccct ctgttagata ccgaggggtt
    24061 ttacctgctg gcgtggctta cgccacacca gcaacggcta gtccgtgccg tcgcctttcg
    24121 cttcgcacgt cgccgtcccg tcctgccttg cttgtgcaag agaaccgata gtcttcaaaa
    24181 aatcatgtcc tttcgggact aagggcgtgt aaaattcgct tatcacgctt gttcctacat
    24241 cacaatagcc acgacccttg atacgcttct ggaaaaactg ctttttcaca tcagaaccga
    24301 acagcatacc atatcctaac tcgctgatac gccccaatcc tacacggaaa ttgaagttat
    24361 ctctgatacc gtccgagaaa tactttgcgt ctggacgctg gcaggcaacg ataaggaaat
    24421 aacctgcttg tcgccccagc ataacgattt tctttaactg gctaagtagg ctcacgcttt
    24481 ctttcgttcc cagcatttca aaaaaagcca catattcatc aaagataaga aagcagggtg
    24541 gcagtcccag ataggcgtag ttttcgcctg tcttgtagtt cgagtgttgc ttcatttcct
    24601 cacttcgttg taccatgccc tcataaaagg aattgacgca atctatcatt tcttctttgg
    24661 tgtggtacac gtttcccatg actgttccca aatctgcaag gtcagcgttc tttgggtcta
    24721 agatgtaaag gacagcgttg gtatgcagta aggcttcaat gagcgtcagc agaaagtaag
    24781 tcttaccgcc accagtccca ccagcaatca gggcgtgagg gagtgcgtca tattcccaga
    24841 caagattttt catcagtcta agacagccgt tttctgcccg tacttcatca atggtaatgc
    24901 ggtttgctat catatcataa agcagggtat attcaatata accgtcatgc agtgttttgt
    24961 cggttagctc acaatacaag ccactttcca atttatcctc taaccgtaaa agctggtctt
    25021 gatatttccc cagcgtaatt tcacagcgga tatgaagcag tcccttttcc atttgataat
    25081 aaatctttgg aaaccagacg attttttccc ttgatctgct ttgcaggtca gtaaaaaaac
    25141 cgctgtcttg tacggtatcg gcttcatacc acttattttc cagtatcatt cttgccagct
    25201 tttgacggtg caggagcttc ttgaaactgt cgtaacagaa gcgataatac agaaaagcga
    25261 ccaatgcaca aacaccagtc gctatcagta tggttatcaa gttgtaaggg gataacgtca
    25321 agcctttttc caacaagctg aaatgttccc aatcggtacg catgagctgt ttgatattca
    25381 gtagcaggag aaccgccacg aatacaaaca gaagcgtccc tatggaaaag tgatagacaa
    25441 ggtgcttgtc gctggctctg atacggtgtc ctttgttcca aatcttacgc ataaatcaat
    25501 cactcctttc tgggtacgaa aaaagcagtc aatcctaaga ctaactgcta gttacgattt
    25561 atttgaaatg tgaaatcttt ccgtattttt cgtatgaata tttaagcatt tgataaatat
    25621 cttctctaag aatatactgg tatgtttctg agattgtttc atacaccgga ataggaattg
    25681 cttttgatga agtcctttgg ttcgatttta tcaacactct ttttgctggc ttagatactg
    25741 ttttatgaac ccaatccgaa ttcaataaac agtgtcccat aggtgtacca gaactgcaat
    25801 atggttgatt ttcattctta ataaatctaa gcaataattt ataatcactt gaagataagt
    25861 tatccacatc tgtccaaaga aattctaaac tctgttgcca ttcttctgct tgtctctgtt
    25921 tgttacgttg ttccacacct tttgaataat tatgatactg tattttctgt accaaccata
    25981 ttactaattc gatgattaga aaccatacgc agaataggaa aacagcatac ccccaaatag
    26041 tgaattttac caataactta ttatccgtag gtgtaacgta ataagctata aaagaaaaaa
    26101 ctattgcaat aactgtcggt actaaatact tttcaagaaa tatctttaat aaatctaata
    26161 acttttcact catgcatcta tgccccttaa ttaaaaaatc acattaaaaa tattatacga
    26221 tttcttaatt gcagaggcaa tcaaaatata tttgatgaac taattatttt ttcgtttgtc
    26281 cctgcggtgt atggttctgg ggattgcctg cgttttgatt gcctttattc ttcaacacaa
    26341 tatcctctgc ctttacatac cagtccacat ctgcacccgt ataggtcttt cttgataccg
    26401 tatcggctac tggattgaca agctccacaa ctgcattgta cggaaattcc cttaacggta
    26461 cttctggcgg tacggacacg gggataattc caccatgcag actgcacttc aaatcataga
    26521 tacgcttttt aagctgagta ctcggtgtcc cgtcctcgtt ctgcaagaac acatcacgca
    26581 ccgctgtaaa ttttaattct ccaaatgttt tctctttgtc aatgacaaaa ccgtttgata
    26641 atctcataaa ttcttcactc ctttactttt cttcaactgc cacaatatca tcagcaacta
    26701 atacataatc ggtatgtccc atatccccga ttgcgtaacc tctgccgtat aacttcggat
    26761 tgacaagttt tactttctgc tcatacttga aatgcttttc accagcctgc acgggaattt
    26821 ctaccacaac attttcacct ttctgtacgt cagaataaag gttatagctt cgtctggcta
    26881 aaaccctgcg gttattttta tctctttcaa agataggctc gctttcgcct gcaaattcaa
    26941 gagttccaaa agactgtgcc atatctggca cgacatattt catttccata ttgttttacc
    27001 tcgtttcttt ctatatttta taagttgatt gattgtgatt tcttattctg gcggtttggg
    27061 aagtaccagc aatcccaccg atagtaaagg gtaaaataaa tgcttacgca cccttgacta
    27121 tccgtgttct tgcaggttgt tggcagacaa gccagaataa gcggaagtct gccgtttgac
    27181 agcttcggcg gagagcgtga ttttcctttt ttcatgctat taccgcccta tgattttttc
    27241 attttacggc gtttgtaaag atatgttcca gcaagcccac caccagatgt aagcaacatc
    27301 acaagaaatg caatgacatt tgtactgtcg cccgttttgg gactgtcgct aggtctgcta
    27361 ggttcttctg gtgtctgtgg cgtttctggt ttctctggtt cttctgggac ttctggtttt
    27421 tccttaaatg taatcgtctg tccctcttcc tcaatgtcct tatgctcggt aactttcttc
    27481 ggctcgtctg gattgcttaa atcgtataat tcctcaaaag ttacaagctg tttgccgtca
    27541 agagaagtag cgtcaaaggt aaaggcaact tccactttca tagtttcgct gtcagcggta
    27601 aaagtataat cactttccac acgttttcca ttgataagaa gctctgcatt tttttctttc
    27661 aacatctgcc agcctacaag tttatactgt gtaccgactt ctaaaccctc taaggttacg
    27721 gtatcaataa ttgtaatgtc ttttccagct tcaagctctt tgttgccgtc cttgtcggta
    27781 gcagtcgtat gtatcttgat gatacgctct gtaatcaata cagtttgccc gtcgtcctca
    27841 atgtccttgt gttccgcaat ttttacgggt tcgtctggat tgcttaaatc gtataattcc
    27901 tcgaatgtta ccagattttt accgccaagc tcggacgcat tgaatgtata agaaatttcc
    27961 actttcattt cttcatcatc agcgacaaag gtatagtcat tttctacacg tttcccgtca
    28021 ataacaagct cggcattttc ttcctttaac atctgccagc cttttaactg gtattttgtg
    28081 ccttttgtaa gtccgtctaa tttgacagta tcgacgattg ttacctcttt tcctgcaagg
    28141 atagtctttt cgccgttctt actggtcgct gtggtatgga tagatatttc tttttcgtat
    28201 tcatcagtca aagtccctaa gtcaatcaca aggttatttc ttgataccac gatttcaaaa
    28261 ggtgggataa gctcaaagcc tttgttactg tcagagcgta attcttcaat gatgtaagta
    28321 tcataaggca atgcaccttt gctgtcgtcg ggttcagaag ttccaaacca tacaccgtcc
    28381 tcacttgttt tgcctgcgtt ggtattgtgc ttatgtgaag cccagtcagc agaagtggag
    28441 aattgcccat tatcatcagt taccacaata tgactttcgc ccgttgtctt gcttgtgatc
    28501 ctaaagggaa catcagcaag acgtttgtgt gtgcctgcac cgatttttac gccctcaata
    28561 tctccacgct taatctgatt gtagatagaa tgggcttcgt cggttaagtc catgattttt
    28621 tcgttttctg tgattgcaaa atcaatcggt tttgcaccgt ctgtcaaata tccgtttgga
    28681 gcttcacttt caacaatacg gaaatttcca taaggtaaaa ggtcggcaga agtagaagcg
    28741 acaccctcaa tatccgtatg gattgttttt accacttcat ttttcttgta tagcttaccc
    28801 tcaaccaata ctgcattatc atttaaggaa atgatgtcaa aagcagtatc tttcaaggta
    28861 gcacttcctt gtggtttggt atcgcccgtt tctaaatctc gtttctgaat tttgacaccg
    28921 ccacggataa ctttatctga tacagaaaac tggttacttc cagataatac ggcaaggtcg
    28981 ccgtcctcgg taatctgtgt tacatataag cccttaatct gttcggactt atcgccagcc
    29041 tgcatatatg caccgtcaag caagtagccg tttggagctt ttgtttcctc aacggttaat
    29101 gttcctaaag gaagaaccgc cttaccgtcc tgcatataga agctgtcgcc agataccttg
    29161 tatgcgtccg ctaattttgt gatgtaatgg gttgtcccgt cgctgtcagt ttcagcgatt
    29221 gtctttgtaa cccatgtacg agtagcttcg gcaggaagat tgtctttgtt atagaagcca
    29281 gcataatact tccatgtaaa ttccgcacct gctagagaag cgttcccctg cggattgtct
    29341 ttctgtgttt ccatgtcaat cttgaaaagc tcaatcaaag tatccgttac ctttggtgta
    29401 tcagatactt ttaaggttgc tgtttcgcca gctttgattg tcaatggata gactgtttta
    29461 tccactttat atcctgctgg tgcagaaagc tctttgatat atactgtccc tgctttgact
    29521 tctacaacat tcgtatttcc gttttcatca gtcgtaaggg tggcaagctg ttttgtgcag
    29581 tccttatcag aaaagacacc ataagttgca ccagagatag agtaatttcc gttaccgtct
    29641 gtaatgctgg tattactgga agtcttttgc agcttcgcat ttccgacatt cagcttcgcc
    29701 cagaattgtc ccaattcctg cccctcgcca gagtagatat aaccgccaca ttcatagcgt
    29761 cctttatttt ctttgacaaa ggcttttgca ccagcgaaaa cttcgtcctg cgtagctttt
    29821 ggtatttcat cataagaagc tcgcacgttg tcgcattgcc agccaagatg tacgcttaat
    29881 ctctgccaga caacacattg ttccaacaga taaacttgtt tgtaattcaa ttccttgtga
    29941 gcttcgccat actgtttgac atactctaag gataacgcca catctgaaat ctggtcggca
    30001 ctcatgcgtg agcttgcgtc agttctggtc ttgtaaccat tcttgaaatc tgtattgatg
    30061 tcaatacaat aggcagtttc gccctcaact ttcatcatgc cctcattgaa tgtagaaccg
    30121 atagaaccgt cattcattac tttttcaatg ataccgacac gctctgcact ttccgtccag
    30181 tattgcttgc tttctgcatg aacgggtgta gtcggtaaag cagtaacgat agttgcaaga
    30241 gctaagaagc ccgtacacaa tcgttttaac atctttttca taaatctaat gctcctttca
    30301 ttttgggtat aaaaatagac gctcatttct gaacgtctta catttaaaaa gggatactat
    30361 tttaacaagt acccctaaag tttagtattt aattgtttgt gttcccacac tctatatcaa
    30421 ctactaccga aacattgata tagtttttta tctctttatt tctgatttta tcgccctcgc
    30481 tcgtaaagac gatatgcttg aaaaagtgcc taaaattaag gatttctaca tagttcgtct
    30541 ttgtagcaac acacaagtct ccacatgtgt agaatgaggg aacatatcta caggttgtgc
    30601 ctcaacacat ttaaatccgt gaacatttag ataatctaaa tcccttgcta aagtagaagg
    30661 attacaagaa acatagacaa ttctttccgg attcattgaa acaatagtct ctaatacctt
    30721 ttcgtcacat ccctttctag gtgggtctac aacgactaca ttggcagtct taccctctct
    30781 atacatctta ggtacaattt cctctgcctt tccaacataa aattcagcat tatgtatatt
    30841 gtttaattta gcatttatct tagcatcttt tatggcatca tctataattt ctattccata
    30901 aaccttttta gccttttgtg ccaaaaataa agatattgta cctatcccac agtaaatatc
    30961 aaaaacagta tcagtatcat ttaaatttgc atattcaaga gccttatcat acaatacttt
    31021 agtttggaca gggtttactt ggaaaaatga taatggtgaa atctcaaaca ctaagtctcc
    31081 tatataatcg tttatttttc catctccata tataactata ttttctcttc ctagaatagc
    31141 attcgtatct ttttgattta catttacaac caaagtctta aatccaggta tattttcctt
    31201 aagcactgaa gctaattctt taagatatgg aagtttgttt ccatttgcta ctaatacaac
    31261 cattacttct ttagttgtaa aacctatttt tgttactaaa tgcctaatca accccgtgtg
    31321 agtcttttca tcatatatgc ttaccttata ggcccttata tatgttttta ttattttaac
    31381 aattttatca tttatctcat gttgtattac gcatttttct gtcgatatta catcatgagt
    31441 cttcttttta tagaagccaa taattggaat accattattc ttttgtattg gaaattgtgc
    31501 tttatttcta tatctatatg gattatccat tcctatagtg tcatgaactt ctacatcctc
    31561 taacttacct attctcgaaa gtacttgttt aacttcattt gttttcattt caagttgttt
    31621 attataatct aagtcttgta tctgacaccc tccacaatcc ctaagattgt cactacacac
    31681 tcttttcacc ctgaatggag acttttctat tatctcaaca atatctccca cagcataatt
    31741 tttctttact ttattaatcc taactttaac tttatcatgt attaatccgc cctctacaaa
    31801 aactgtaata ccattgtact ttcctactcc tactccgcct tgaccaatgt ctactatatc
    31861 tacaatatac atctcgtttt tcgaaagcat aaatcaccct ccaatcaatc tattatattt
    31921 aaatctttac aaactttttt ctttaaattt atattaaaat attgctatta attaaactat
    31981 attttttact aagctacaat attttatttt accaaaaaaa aaggagctta taaagcccct
    32041 tagtaaataa atttattatt tttcttataa aactctagtt gaacctctat ataccacgcc
    32101 tcttgatgca tccacagtta caacttctcc atcttttact agatttgtta tatcagctgc
    32161 agacacaata actggtatat ctaaatttat acctacaata gctgtatgtg atgtcattcc
    32221 accattctca gttactacag ctcctgcttt ttctatataa ttgttcattt cagcatcagt
    32281 agcagtagaa acaagtatgt cgccttcatt aaagtctaca caagcatttc cattctttat
    32341 tatacgaact ttaccttcta ctgtttgagt acctacacct ataccttgca cgatttcttc
    32401 gcttattaca tgaaccttta ttaaattagt agttccactt actcctactg gtactccagc
    32461 agttataact actaattccc cattatctat ataatcagct tgtctagctg cctcaattgc
    32521 cttttctata acttcatctg tatttcctgc aacagaagac ttaactggat atactcccca
    32581 tgtaagagct aatttattca tagtcttttc attatttgta gtagctatta ttggagattt
    32641 tggtctgaat ttagatacca ttctagcagt atatcctgaa gaagtagatg ttattatagc
    32701 tgatgcattt aaatctactg ctgttgtaca agttgcatgg cttattgcat ctgttacagt
    32761 aacattgtta gtaccatttt cctttagtaa tctatcataa tctaaagtct cttctgttct
    32821 ttttgctatt gtagccatca tttttactgc ctctacagga tattttccag cagcagtttc
    32881 tccagataac attatcgcat ctgttccatc atatatagca ttggcaacat ctgtaacttc
    32941 tgctcttgtt ggtcttggat ttcttatcat agaatcaagc atttgagtgg cagtaacaac
    33001 tggctttgct aattcattac atttttttat catcattttt tgaactattg gcatctcttc
    33061 agttggtatc tcaacaccta agtcacctct agccaccatt ataccatcag aaacttttaa
    33121 tatttcatct aggttctcta caccttcttg gttttctatt ttagatatta tttgtatatc
    33181 agttgcatta ttgttctcta atacttctct tattgctagt acatcagatg cctttcttac
    33241 aaaagaagca gctatataat ctattccttg acttattcca aactctatat cacttatatc
    33301 tttaggagtt attgctggta aatttatctt aacaccaggt acatttactc ctttatgatt
    33361 tttaactatt cctgaatttt caacaacaca aactatatct tctccattta tttcttttac
    33421 tcttaagcca actaaaccat catctattag tatagaatct cctgatttaa cgtcttcagc
    33481 aagaccttta tagcttactg tacacatttc tttagtcccc ataatatctt tcatagttat
    33541 agtgaacttt tgcccttctt ctaaaaatac ctctgggtct tcaaagttac ctgttctaat
    33601 ttctggacct ttagtatcta ataatatagc aactggctta tttaattttt ctctaacttt
    33661 ctttgccata tcaattcttt ctttatgttc ttcatgcgaa ccgtgagaaa agttaaatct
    33721 acacacattt agaccatttt gcataagttg agttaaaact tcctcacttt gtgatgctgg
    33781 acctagtgta caaacaattt ttgtcttctt tacattattt aacatatttg ctcctcctga
    33841 gatattattc cacagattta tttttaatac taaaacttat atagataata tttttgccat
    33901 ctcataagct tctttatcaa atactttctt ttgagctaaa gcttcactta tttctaaatc
    33961 aattattttg ttttctttta ttcctacaac tctagctgat tttccatcta ataataattc
    34021 tactgctctt actccaagtc tacttgctag tattctatca gatacagttg ggcttcctcc
    34081 tctttggaca tgccccaata cagtgactct taaatctgct cctgactctt ttttaagttc
    34141 cttttctaaa tcactagcac ttccgactcc ttctgctaaa acgattatac tatgtctctt
    34201 acctcttttt tgagtagtct ttaatcttag agcaacttca tctactgtaa tttctacttc
    34261 tggtactatt atagtttccg ctcctcctgc aagacctgca tataaggcta aatctccaca
    34321 gtgtcttccc ataacttcta ctatgttaac tctttcatga gatgatgatg tatctcttat
    34381 cttacctata gcatctataa tagtattcat tgcagtatca aatcctatag tataatctgt
    34441 atatgctaaa tcgttatcta tagttcctgg tatgcctata gctgggaatc ctaagtcact
    34501 aagtttttga gctcctgcaa atgaaccatc tccaccaatt acaactaagc aatcaatttt
    34561 atacttttta agaattttta ctgcttttaa tctaccttct tctgttttaa attcttcaca
    34621 tctggaagat tttaatatag ttcctcccct gtgtataata tctccaacag aagataagtt
    34681 catttcagtt aaatcttctt ctagtaatcc cttatatcct ctattgattc catatacttt
    34741 gcatccataa tatattgctg atcttacaac cgctctaatc gcagcattca ttcctggagc
    34801 atctcctcca cttgttagta atcctatagt tttcattttt tacctccttg gattttatgt
    34861 caatttttta ttataatcat tgttattgta ttctaatgga ctttttttgt ccctatatca
    34921 ctttttaatc ccatcaagac taaaaaaatt aaatgtcctt aaatgtccat tttgtgatat
    34981 tatatcacaa aacggacaat atttttcata ttattataat atatttagat ttttatataa
    35041 aaaatcaagc ttttactttt atgttatctt taggaagtaa ctcttctaag ttatttataa
    35101 attcatcaca tatacaagtc aatgccccgg tcattttata tgctttacgt ttaccattta
    35161 tattttcatc catagggtac aagtatatac tgtcattacc tggatattgt ttggctattt
    35221 ctacaatact attagttgta tcagtatcag acatactatc tattttcaaa tacaattttt
    35281 taccactttc tggcttaata ttttctacat ttctagttct tcctgaattg tagtttctat
    35341 ctgcaaagtc agtatcatca tttatatctt ttatctctct agctataagt tttgcatttt
    35401 cgccttcttt tatacttaat gttcctttta catatattat attatcttca ttcaaaatga
    35461 cattatactt ttgtaataat tgaggaaaaa ctattatttc tatagcacca tacaaatctt
    35521 ctaattcaat aaatgccata atttcatttc gttttgttgt tttaattgtt ttagttacaa
    35581 tcattccacc cattataaca tctgtttcat tcatttgtag gtatgactct tcttcctctt
    35641 ttaatgaatt taattttcca ttatccatag atgttttttc ttgtagttct ttttcaaatt
    35701 gtgataaagg atgtccactt acatacattc ccaagacttc tttttcaagg ttaagtctct
    35761 ctctttcttc gtactcttgc cttttaggta aactgtatat ttgttgaaaa tcactatctt
    35821 cctcactatc tccaaaagca tcaaataaag aaatttgacc ttgaacattt ttctttctat
    35881 ccatagatac actttctaat aaactttcat aaccagccat caaactagct ctattttcac
    35941 ttatattatc aaaagctcca cattttatta gactttctat tactctctta tttgtatctt
    36001 tttggtctaa tcttttgact aaatctacta aatctacaaa taatccatta gattctcttt
    36061 cagcgattat attgtttatt atatttacac caacattttt tacagctgct agaccaaatc
    36121 ttatattgtc gccttcaact gaaaactttg aaaaactttt gtttatatct ggaggtaata
    36181 catctatttc tagtgcttta cattctctta tatactcaac taccttatca gtatttccca
    36241 ttacactggt tatcaatgct gccataaatt caactggata atatgttttt agataagctg
    36301 tctcatatgc taaaacccca tatgcagctg catgtgattt attaaatgca tatctagcga
    36361 agtctatcat atcatcaaat attttattag ctatttcctc ttctacacca tttctgatac
    36421 accctgcaat ttctatatta ccatcttcat caaatttacc atgaataaaa tattgtcttt
    36481 cttcttccat cacgtccatc tttttcttac tcatggctct tctaactagg tcacttcgtc
    36541 cgtaactata accacctaaa tctctaacga cctgcataac ctgctcttga tataccaaac
    36601 aaccatatgt aacttccatt ataggcttta atttttcatg taaatatgtt acatcttttg
    36661 ggttgctttt attttttata tatgcaggta tagaatccat tggacctggt ctaaataggg
    36721 atattcccgc tacaatatct tcaaaattgt ctggttttag ctgtttcata aaacttctca
    36781 tgccagcact ctccaattga aacaccccta gtgtatttcc tgatgaaagc atttcatata
    36841 ctttcgggtc gtcataatcc atctttgaaa agtctatttt ttctgtgtaa ttttttataa
    36901 ctctattttt ttctattaaa tcaagagtat ctcttataac tgtaagagtt cttagcccaa
    36961 gaaaatccat ctttaaaagc ccaagttctt caagtgttgt catagtaaac tgtgttgtga
    37021 ttgcatcttg atgtttgtac aaaggtacat attcatctac gggatttttt gatataacaa
    37081 ctcctgctgc atgagtagaa gcatgtctta gcattccctc tatctgtttt gatatattta
    37141 ttatttcttt tgtttccggg tcttggtcat acaattcttt aagattagga tttgtatcta
    37201 gggccttatc tattgtcatt cctaaagcaa atggtatttc cttggctacc ttgtcaactt
    37261 tattgtagcc tatatccaat acccttccaa catctcttat tgcagctttt gcacccattg
    37321 ttccaaaagt tattatctgg gctacatggt catcaccata ttttctcttt acataatcta
    37381 taacttcttc tcttctctca taacaaaaat ctatatctat atctggcatg gatatacgtt
    37441 ctggatttaa aaaacgctca aataatagag agtactttat tgggtctata tcagttattt
    37501 taagtgtata tgctactatc gaaccagccg cactacctct acctggtcct actataatat
    37561 tattttcttt agaaaagttt ataaaatccc aaacaattaa aaaatactct acataaccca
    37621 ttttttcaat tacatctaat tcataattta atctctctag tatctcattg ctaggattat
    37681 catatctctc ttttaatcca ttaaaacaca actcccttaa ataagtttct ggtgtatatc
    37741 cttctggaac atcatactta ggaagatgta tagtattaaa atcaaactcc acattacaca
    37801 tatcagctat tttcacagta ttgtcaatag cctctaatgc atatggaaat aattcttcca
    37861 tttcttctct tgattttaaa taaaactcat cactaccaaa tctcattcta tttgggtcat
    37921 ttaatgtttt tcccatttga atacacataa gtacatcatg tattttcgaa tcttctttat
    37981 ttacatagtg aacatcatta gtagcaacta gtggtatacc agtctctttt gataatttta
    38041 ctaaaccaga gtttacttct ttttgctcag gtaaattgtg atcttgaatc tccaaaaaga
    38101 aattttcttc tccaaatatg tctctgtagt caagagcaaa ttgttttgct ttttcataat
    38161 ttctattcat taaagcacgt gctacatctc ctgcaaggca tgcagaaagt gctataatcc
    38221 ctttgttatg ttttttaagt tcttccatgt caactctagg cttatagtaa aaaccttcta
    38281 cgtatgaagt tgaaactatt tttattaaat ttttatatcc ttccatattc ttagctaaca
    38341 gaactaaatg tccttgatat ttgtcatagt ttgggtcttt atctctcaat ccacgtgcag
    38401 ctgtatagac ttcacaccct ataattggct ttattccttc ttttatagct gttttgtaga
    38461 aatctataac accaaacata caaccatggt cagttatagc tattgagctc atgtttagtt
    38521 ccttagccct ttttataagt tttttcaccc tcgaaaaccc atcaagtaaa ctatactcag
    38581 tatgtacatg aagatgagca aaatctttgt tcattaaatc acctcctgct ttataatttt
    38641 aattttacca caaatatatg tactaaaaca aaaacagaga cttttatctc aaaataggaa
    38701 ttaacttcaa catataccat tatatgttat aaattaatgc ttattctgag attgtcccct
    38761 caatttattg ctatgatttt tttttcaaat ctgttgcaat ttcttctatt tttctaagtc
    38821 tatgattgac tccagatttt cctattggtg gttctaacat ctctccaagt tctttgagag
    38881 tcatatcctc atactcgatt ctaatcttag ctatctcttg taaattttct ggcaaactgc
    38941 taattcctat agtctcttgt atgagcttaa tattttctac ttgtcttact gctgcattta
    39001 ctgttttaga aaggttagct gtctcacaat ttactatcct gtttacatta tttctcattt
    39061 cttttactat tttagtattt tgaagactta aaagagcgtg gtgtcctcct ataatactca
    39121 acaaatctga tatttgttca ctctctttga tataaacaac gtgattattt tttctagcca
    39181 caattttact attaaaacct aaagagttta taagttcttt tagagaatct gcaaactcct
    39241 cattattagt tacaaattcc atatgatagt tcttttctgg gtcgcttata gaacctccac
    39301 caagaaaagc tcctcttatg aaagctctct tacatccttc atgcttaatt aagttctctg
    39361 aaattttgtt tatagtaaaa aatccttctt tatcttcaag tatacctaac ttttttaata
    39421 acatttctga acccatttcg cttgttacca ttaatacata actgttattt ctctttagca
    39481 tttgattctt atttactgag attgtagtat taatattaaa attctttttt aataatttaa
    39541 atactttacg ggctattgaa tttaactctg ttgttatctt tagatttagc ttcttatatc
    39601 caacaatttg tatacttccc cctgatttaa ctagagctga caattcagct atattacaac
    39661 attcattccc agatataact cttgctagtt catttttggt ttcagttgaa aaagacatat
    39721 ctatcaccta ttaactaatt tttctttaac ctttttttta tttcttgtaa ggcaaccttt
    39781 attatctcct tttcatcatc atacctagct aagtctacta ctctatctag gtggataaat
    39841 ttagcatcaa taaagccttc ctctttttgt ggaatagtat ccatattctt tgcttgcatc
    39901 aaataccaaa aaactgtttt atgaactgct ctattttcat cccagttttc tttgaatgta
    39961 tagtgtattt cacctaaata tttcaatata tctgctttta ctccagtttc ttcacttact
    40021 tcacgtaatg ctgcctcttg gttattttcc ccttcttcta cttttccctt gggtaacacc
    40081 caatctccat tgaacttcct tagtaaaaga atcgtattgc caaacaggac tacaccacca
    40141 gcactaacct cttctctcat cgatgttcac tccttaaaac taacataatg ataaggattt
    40201 ctacacaatt ttttaaaatc cttttttact aacttttgtt gtaattatga tttagggcta
    40261 actcaataac tatgttagat atatactttg catcatgtct tatataatta ttttttattt
    40321 caattaaatt cttctctaca gtttttatac ccatctcttt taatttatct ttttgctttt
    40381 tatctaacaa gacttgctct gctccatctt gtttatataa gtcaagcatt tcttctggca
    40441 atatctcatt atttgcgatt acataatcta ttaaattctc atcagtgtgt tttactatag
    40501 cattaacatg ctctaataca ttgtaccctt ctgtctcacc tggttgagtc attatatttg
    40561 ctatatatac ttttggagct acagacgatt taatggcgtc tacaatcccc tctacaagta
    40621 aattgggtat tatacttgta tataaacttc caggacccat aattataatg tcagcatcat
    40681 aaatagatgc aataacatcc tttaatggtt ttacatcttt tggatttaaa aaaattttat
    40741 caatagagct tttttgaatt tttgactctt caggtatact ggattctcca tttattatat
    40801 tgccattttc taatttggca actaaattta catcttccaa agttactggt aaaactcttc
    40861 ctgttatagc aaaaatttca cttaatttat atacagcttt ttcaaagttt ccatacaacc
    40921 cattcatagc agccaaaaac aaatttccaa aactctgacc ttttaaaagc ccttctgtaa
    40981 acctatattg cataacttca ttcattgttg gctctatatt tgcaagtgct aatagacagt
    41041 ttcttatgtc tcctggtggg agcattccta agtcttctct caatacccct gaacctccac
    41101 catcatcagc aacagttaca attgcagtta tattttgcgt agtgtgttta agacctctta
    41161 aaaataccga ttgaccagta cctccaccta tcacaaccac atttggttct ctattttcaa
    41221 caatagacct ttgaaatagt tttacttttt cattttttgc ttttttatat ataaataacg
    41281 atacaattaa tgcaatccaa cctaaaattc ctacaataat aagtaattta gccatgtttg
    41341 cctccattat cgcaacatag agtctctatg tttctttaca actctatatc ccttattaga
    41401 taaatcctcc gctataagat ttgttatagt aactgaccta tgtcttccac ctgtgcatcc
    41461 aacaccaatt actagatgtt gttttccttc ctctatatat tgaggtacta aaaaatgaat
    41521 catatctaaa agtttcacat aaaattcttc actgatttta gaattcataa cataatctct
    41581 aacttcttta tcatcaccag ttttagctct taactcttca acataatagg gatttggaag
    41641 aaatcttaca tcaaatacta aatctgcatc agcaagtata ccatgtttaa atccaaaaga
    41701 taccacagaa atagttaggt ttggattatc ttcaccagaa gaatatattt tctttatttc
    41761 ttccttaagg tctttaggtt tcatgttagt tgtatcaatt atacatgtag ataactcctt
    41821 taaaggttcc attatctttc tttccatttt tataccttct ggaatctgca tatcttttgc
    41881 caatggatga tttcttcttg tcattttata tctctttaac aatacatcgt cattacaatc
    41941 caaatatacc atttcatatt cataattttc tttttctaaa taatttaagc tttcatgaag
    42001 cgcttcaaaa aactttctcc ctcttatatc tatccctaga gcaactttat caatacttga
    42061 atttggttga taacataatt ctgcaaattt agttatcaaa gcaggaggta gattatctac
    42121 acaataaaat cccatatcct ctaaagctct cattgtctca ctttttccag atcctgaaag
    42181 acctgtaact ataacaaatt tcattgctaa ctcctccctt ttatcttatg tttattatat
    42241 tataatgttt tctactaacg atatatccag attagagtca gatatagtct tcatagctag
    42301 ttcaaaatca ccaactcttt ctggacaatg tgcatctgag cctattataa atttattttc
    42361 tagccctttt attttattta attgatttac attcaaaaaa gaatggctac tatttatctc
    42421 aagtctagta tttgtctttt ttgctgtctt tgctacttct tctatataca catcgccttt
    42481 atctcctgga tgagtaatta taaaaatatc attattactt atcgcattta ttacagcttt
    42541 tgtattatat tcttttgctt tttcagtttt aaaaatatag ttattacaat gatttaataa
    42601 gcttcttaat gatgttggag tagaaccaaa atgatatcca gccataatat aatcaaaatc
    42661 tctaataaat ttatcatcca tatctatatt tcctttatca tctaatatgt tacactccat
    42721 acctaaaagt atatttatat cagtgtactt atcatttaaa taatctactt cttctctcat
    42781 tttgagaata tcttttttct ttacaccata tgcaatatgt ttataactat ggtcagatat
    42841 tcctattgtt tttattcctt tagaaatggc tacctttaca ttatcctcaa tagtcccttt
    42901 gccatgacta tataatgtat gtgtatgata atcagccagt attttcattt gttaatcctc
    42961 tccaagtatt ttgacttctt cttccaacat aaccccaaac ttggcatata cagcactttt
    43021 tataacatat attaaatcca aaatatcttt agcacttgct tctccttgat tgactacaaa
    43081 tccacaatgc ttctcagata cttgtgctcc tcttaaagta agacctctca atccagtttc
    43141 ttctattaat ttacctgcaa aatatcctgt tggtctctta aatgtacttc ctgcactagc
    43201 aaaatttaat ggctgtttag ttattcttct cgtcgctaaa tcatccatta aactttttat
    43261 ttcatcataa tttccttctt gtaattctaa ttctgctgat aaagctatat atccgttttt
    43321 cgatattata ctttttctat acccaaattc catctgttca tttgataatt caaatatatt
    43381 gccttccata tccatcagtc ttactgactt cactatgtcc ttcatttcac caccatatgc
    43441 tccagcattc atggctaatg ctcctcccaa tgtgccaggt attcctgatg caaactcaaa
    43501 cccttttaat tcttgctttt ggagagcttt ccctaaaact gataataaag ctcctgactg
    43561 agcagtcatc ctagtatcat ttatctcata agaattgaaa ttatcagcta attctattac
    43621 cactcctctt attccaccat ctttaattaa aatgtttgaa ccatttccaa ttattaaata
    43681 cggaatagat tcttttttta ccaacttaag tacatttttt aattgttctt cagttctagg
    43741 tctaactaat atatctgctg gtcctcctac cctaaatgaa atatgttttt tcataggttc
    43801 atcgactttt atatcctctt tacttaatat atttaataaa tttatatata tatcttgatt
    43861 attcattgta tttcctccca aattatactg aactttagct gtcattatta tttaaattac
    43921 attagtatat agtattaact atattcaata atatatgatt ttgcttatca tatagatttt
    43981 atattttaag cttcttaatt acaaactata ttcaataaat atataataaa gtaaaaaaaa
    44041 aaaaataaca acatataaaa gaatataaat aatttaaaaa aagcatcaat atcactgata
    44101 tcaatgcttt aaaaggcaat ttaattcgta ttatttatct aactattttt gacatagcaa
    44161 agataataaa tccctccaac tactatggaa cctccaacga tatttccaag tgttacaaat
    44221 accaaattag ttataaattg aaccatagtt atatttgctc ctaatgccat tcccattggt
    44281 ataaaaaaca tgtttgcaac actatgttgg aatccacata atacaaatag cattattact
    44341 gtccaacatg aagccaattt tgacattata tcttgagatg ctgtagccat ccatacagct
    44401 aaaacgacta gaatattaca aagtattgct tttaaaaaca ttgatgctat agttgaatta
    44461 accttagctt ctgccacagc tatagctttt gtagctgcat ctcctgtcaa tgtagaagaa
    44521 taaaacacaa gtgcaactag caaaattgaa cctactaaat ttgcaaaata aaccagtccc
    44581 cagtttctta acatttttcc taatgttatt tttttgtcca taagtgcaat agccattaat
    44641 gtgtttccag tgaataattc tgctcctgcc actactacaa gcataagacc tactggaaag
    44701 accatagcac ttaaaaagct agctacccct gggtctatct tgccaaaagt ttgactaact
    44761 atcatactcc ccaatgcccc aaaacctata aatgctcctc caaataaacc taatagaatt
    44821 gcgttttgtg tcgaaatatt tgctttttta atgcctgcac ttatagttgc ttgtgcagtt
    44881 tccccaggtg tcaagaatcc tttactcata aatccttccc ccttaaaaat tatactttcc
    44941 cttgtctttt tatggcaata attatataat aacaatatga tatataataa attttattaa
    45001 cataaaaaat atactatata cttatatata cttgtctcat ataattatac tatttattat
    45061 ataacaactt tgttaatttc gaaataattt atattgaatt tgaaaaattt accttttgat
    45121 tatttatttt actttttaat tattg
//
LOCUS       DAFKMX010000008        43763 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFKMX010000008 DAFKMX010000000
VERSION     DAFKMX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.008"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            384..1013
                     /locus_tag="LZB08_000352"
     CDS             384..1013
                     /locus_tag="LZB08_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7313873.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(1127..2641)
                     /gene="glpK"
                     /locus_tag="LZB08_000353"
     CDS             complement(1127..2641)
                     /gene="glpK"
                     /locus_tag="LZB08_000353"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBM7313874.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2885..3427
                     /locus_tag="LZB08_000354"
     CDS             2885..3427
                     /locus_tag="LZB08_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7313875.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3441..5933
                     /locus_tag="LZB08_000355"
     CDS             3441..5933
                     /locus_tag="LZB08_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7313876.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6353..6751
                     /locus_tag="LZB08_000356"
     CDS             6353..6751
                     /locus_tag="LZB08_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7313877.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6968..7177)
                     /locus_tag="LZB08_000357"
     CDS             complement(6968..7177)
                     /locus_tag="LZB08_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313878.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(7209..7418)
                     /locus_tag="LZB08_000358"
     CDS             complement(7209..7418)
                     /locus_tag="LZB08_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313879.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7717..8136
                     /locus_tag="LZB08_000359"
     CDS             7717..8136
                     /locus_tag="LZB08_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313880.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8364..8819
                     /locus_tag="LZB08_000360"
     CDS             8364..8819
                     /locus_tag="LZB08_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313881.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(9027..9287)
                     /locus_tag="LZB08_000361"
     CDS             complement(9027..9287)
                     /locus_tag="LZB08_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313882.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9550..9903)
                     /locus_tag="LZB08_000362"
     CDS             complement(9550..9903)
                     /locus_tag="LZB08_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313883.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9920..12787)
                     /locus_tag="LZB08_000363"
     CDS             complement(9920..12787)
                     /locus_tag="LZB08_000363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313884.1"
                     /translation="MKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKGTHGISV
                     NSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDVGQLGVG
                     DEINRKVFAKINIDNIKSISVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLGIGDNVN
                     RNVFTKINVENVKYVTVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNIFTRIPI
                     NGVRDVYLCNDVSIIVKNDNTCYVCGFVNGYFGFTESSISTFTKINIENVKSVVTAGS
                     EATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTSAILTND
                     NKLFVGGLSGYGSITNNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDTYGISDE
                     YYMDIPVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIHHRYDND
                     KNEALEGSLYSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIFKDNFKY
                     TSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTFTEVNISD
                     VKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVKDISIYN
                     SMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIITTFTKVLTNVKEIKSHNDDHILVI
                     KNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYMITKVDT
                     SKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFEIPQNKI
                     VLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINITENNTDL
                     IVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSYQEIYEL
                     EEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(13124..15244)
                     /locus_tag="LZB08_000364"
     CDS             complement(13124..15244)
                     /locus_tag="LZB08_000364"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313885.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDSSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTMVILGNGDVYGLGNNGYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLINKSASQYT
                     EISISNVTDAYSAYNATFIVVDEKKVYVTGINKNYMLGVGVNNEAYTANLNLLSTWNY
                     INSIGTSYSRVSCTNDITKINNIVIYEYVTIFCTNIGSFLTGYHGTLWTKPTDSSYRV
                     QYQGISYAGYLDSYIYNYYPTRCT"
     gene            complement(15261..16244)
                     /locus_tag="LZB08_000365"
     CDS             complement(15261..16244)
                     /locus_tag="LZB08_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBM7313886.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(16245..16943)
                     /locus_tag="LZB08_000366"
     CDS             complement(16245..16943)
                     /locus_tag="LZB08_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBM7313887.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16940..17992)
                     /locus_tag="LZB08_000367"
     CDS             complement(16940..17992)
                     /locus_tag="LZB08_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBM7313888.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17985..18413)
                     /locus_tag="LZB08_000368"
     CDS             complement(17985..18413)
                     /locus_tag="LZB08_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBM7313889.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(18413..18739)
                     /locus_tag="LZB08_000369"
     CDS             complement(18413..18739)
                     /locus_tag="LZB08_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBM7313890.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18755..20287)
                     /locus_tag="LZB08_000370"
     CDS             complement(18755..20287)
                     /locus_tag="LZB08_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBM7313891.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGTEFSAIFTA
                     YYPGPGIEGGDTDCREKKLNPSKKTCAAPMVGAYEKPYYTKEFLSKHPLFKYGDEVSI
                     VTGVSGRDGLYKVNDNGSAIIIEKDGTYHIDVLVKNAEEMKRFGKRKGKIIIGGYSGN
                     ASNKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKG
                     KKVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARR
                     VL"
     gene            complement(20569..20991)
                     /locus_tag="LZB08_000371"
     CDS             complement(20569..20991)
                     /locus_tag="LZB08_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313892.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20991..23444)
                     /locus_tag="LZB08_000372"
     CDS             complement(20991..23444)
                     /locus_tag="LZB08_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313893.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23444..23611)
                     /locus_tag="LZB08_000373"
     CDS             complement(23444..23611)
                     /locus_tag="LZB08_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313894.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23638..24084)
                     /locus_tag="LZB08_000374"
     CDS             complement(23638..24084)
                     /locus_tag="LZB08_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBM7313895.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMCYGKNAVIEEVKN"
     gene            complement(24210..24638)
                     /locus_tag="LZB08_000375"
     CDS             complement(24210..24638)
                     /locus_tag="LZB08_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBM7313896.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24652..25716)
                     /locus_tag="LZB08_000376"
     CDS             complement(24652..25716)
                     /locus_tag="LZB08_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBM7313897.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25721..26164)
                     /locus_tag="LZB08_000377"
     CDS             complement(25721..26164)
                     /locus_tag="LZB08_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313898.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTLTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(26413..26853)
                     /locus_tag="LZB08_000378"
     CDS             complement(26413..26853)
                     /locus_tag="LZB08_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313899.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26958..27155)
                     /locus_tag="LZB08_000379"
     CDS             complement(26958..27155)
                     /locus_tag="LZB08_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313900.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEENIEEVFFIQS"
     gene            27324..27644
                     /locus_tag="LZB08_000380"
     CDS             27324..27644
                     /locus_tag="LZB08_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBM7313901.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27726..28232
                     /locus_tag="LZB08_000381"
     CDS             27726..28232
                     /locus_tag="LZB08_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313902.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28933..29688
                     /locus_tag="LZB08_000382"
     CDS             28933..29688
                     /locus_tag="LZB08_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBM7313903.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29831..30259)
                     /locus_tag="LZB08_000383"
     CDS             complement(29831..30259)
                     /locus_tag="LZB08_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBM7313904.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30680..30880)
                     /locus_tag="LZB08_000384"
     CDS             complement(30680..30880)
                     /locus_tag="LZB08_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBM7313905.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(31063..32232)
                     /locus_tag="LZB08_000385"
     CDS             complement(31063..32232)
                     /locus_tag="LZB08_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBM7313906.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKSGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32468..33289
                     /locus_tag="LZB08_000386"
     CDS             32468..33289
                     /locus_tag="LZB08_000386"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBM7313907.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33365..34753)
                     /locus_tag="LZB08_000387"
     CDS             complement(33365..34753)
                     /locus_tag="LZB08_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBM7313908.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(34888..35646)
                     /locus_tag="LZB08_000388"
     CDS             complement(34888..35646)
                     /locus_tag="LZB08_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBM7313909.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGVGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(35648..36364)
                     /locus_tag="LZB08_000389"
     CDS             complement(35648..36364)
                     /locus_tag="LZB08_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBM7313910.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(36366..37073)
                     /locus_tag="LZB08_000390"
     CDS             complement(36366..37073)
                     /locus_tag="LZB08_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBM7313911.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(37189..37725)
                     /locus_tag="LZB08_000391"
     CDS             complement(37189..37725)
                     /locus_tag="LZB08_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7313912.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(37827..38897)
                     /locus_tag="LZB08_000392"
     CDS             complement(37827..38897)
                     /locus_tag="LZB08_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBM7313913.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(38890..39948)
                     /locus_tag="LZB08_000393"
     CDS             complement(38890..39948)
                     /locus_tag="LZB08_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBM7313914.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(39935..40267)
                     /locus_tag="LZB08_000394"
     CDS             complement(39935..40267)
                     /locus_tag="LZB08_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBM7313915.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            40466..41587
                     /locus_tag="LZB08_000395"
     CDS             40466..41587
                     /locus_tag="LZB08_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBM7313916.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(41715..43613)
                     /locus_tag="LZB08_000396"
     CDS             complement(41715..43613)
                     /locus_tag="LZB08_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBM7313917.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        14488 a         7232 c         4866 g        17177 t
ORIGIN      
        1 ttttcaaaaa atctttttac cattttttag ttaataatta attattttca cttaaaataa
       61 gggctttaac cttcattttt aaagtatttt tcatttttaa tcactttata tgcaagacag
      121 aatcattttt cttcattcat ttgtttcttt catctatata attatcattc tttgtaagta
      181 aaatatcttt atatttttat aattaattct ttatatagtt ttaagtataa taaaatcatg
      241 tatactaaat gcatgttaaa atattatttt aaaacatgca caatatttgt acatattgta
      301 gacatataaa cctataaatt atacaatagt attgttaaga ttatatattt ttttaacata
      361 aattttgtat tgggggaaaa acaatggata aaaactatac gatgccaata taccaaaaaa
      421 tagcgttgga tattgcaaat aaaatataca cgggtgaaat acaagaggat tcagtactat
      481 ttggacgctc tgttttggct ggtaaataca atgtatctcc agaaacgata agaagagctg
      541 tcaaaatttt agaggacatt ggcgttgtta aaagcataaa gggtaaaggt gttattgtac
      601 tatctccaga caaagcttct tcttttataa aaaaatatag agatattaca aatatatcat
      661 catataaatc aacactttat aatttaatag atactaaatc taatttagaa aatgaaatat
      721 tagatactat aaataaaatt ttagactatt ctaacagact tgaaataatc aatcctttag
      781 tgcccgttca gttcaccata aattctaatt gcaaatatat tggtcaaaca gctgcacaaa
      841 ctaaattttg gcaaaataca ggtgctacaa tagttgcaat taaaagaggt gaagaactta
      901 taatatctcc tggaccatat attgaatttt tagaaggaga cattctctta gtagttggtg
      961 accaacacat ttacaattct ataccaatgt ttttatatga aaacgaaaaa taaattatat
     1021 aatttagtgt gattatataa aaatgcaaga ttaacaacaa aaaaagagct tcatatttta
     1081 cataaagctc tttttaaatt caaataaatt taactttgat taatttttaa atatccaatt
     1141 catcttcttt ttcccagctt aaagctctcg atacagcttt tttccatcct ttatataatt
     1201 tacatctctt ctcttcactc atatttggat taaactcttt ttctactgac cactttgaag
     1261 taatttcatt tcttccaata tagaatccaa cagacaaccc tgctaaataa gcagctccta
     1321 aagctgtagt ttctactatt ttgggtctat ctatatctac atttaatata tctgattgaa
     1381 attgcattaa aaaattatta ttacttgctc caccatcaac ttttaatgat tttagtttta
     1441 attttgaatc gttttgcatt gcttctaaaa catcctttgt ctgataagca attgattcca
     1501 atgtggctct tactatatgt tctttttttg ctcctcttgt aagtcctaaa attgaacccc
     1561 tagcatacat atcccaatat ggagctccaa gacctgtaaa tgctggcact acatatacac
     1621 cattagtatc ctctacccta tttgcataca tttcactatc ttttgcagat tcaattatct
     1681 ttaattcatc tctaagccat tgaacagatg ctcctccaat aaatatactt ccttctaatg
     1741 catatgttac ttttccatca actccccaag caattgttgt aagtaaccca tgcttagatt
     1801 taactatgtt actgcctgta ttcataagta agaaacaacc cgttccataa gtattttttg
     1861 cagtaccttc ttcaacacaa ttttgaccaa ataatgctgc ttgttggtct ccagcacatc
     1921 ccgcaatagg tattttagct cctgataaca tgcgctcatc tgtatgacca taaacatagc
     1981 tagaaggttt tactaatggc aacatgctta ttggtatgtc caattctcgt aatatatcct
     2041 catcccattc taaatcattt atattgtaca tcattgttct agatgcattt gagtaatctg
     2101 ttacatgtac ttttccacga gttaaattcc atattaacca tgtatctata gttccaaata
     2161 ataactctcc ttgttctgct ttttccctag ctccttctac attatctaat atccatttta
     2221 cttttgttgc tgaaaagtat gcatctatta aaagacctgt tttttctttt attgattctt
     2281 ccaatccttt atttttaagt tcatcacata tttctgacgt tctcctacac tgccatacta
     2341 ttgcgttata aataggttta ccagtatacc tactccaaac aaccgtagtc tctctttgat
     2401 tagttatccc tattgaagca atttcttctg gtcttatacc tgctgtttct ataacttctc
     2461 tcattacccc actttgtgac ccccatattt ccatagggtc atgctctacc catccagctt
     2521 ttggataaat ttgattgaac tctttttgtg caattttcac tatttcgcct tttttattaa
     2581 atagtatagc tcttgaactg gttgttcctt ggtctaatgc cattacatac ttcttttcca
     2641 tcaccatatt ctccttatca tataatattt aatttattaa aaagaaaaat ttaatatcac
     2701 atatttcaat aaataatcat acttaaatac aaatttaata taatagatat ttagtaagaa
     2761 gacacttatt ttctatcata tctatataat aatattatac attatagctt acatattgat
     2821 taataaattt taaaggagta taataaacag tgttcataat aattttaata aaagaggtgg
     2881 taatatgcca aagattttag aaaatgtaaa agaagatata ttaaaagtgt ctcgggatat
     2941 gattttagaa gaagattact cgaatattag cataaggaaa atagctcaaa ggtgtggtat
     3001 aagtgcaggg acagtgtata attactttaa ttcaaaacaa gaaataatag aatatatcac
     3061 aaaatctgaa tgggatttat tgataaggcg aattgaatac tccaataaaa acactgaaga
     3121 ttacataaaa aaactaaaca ttatttttac tgaaataaga aattttataa acaaagttca
     3181 taatatccaa tacaatgact ttttaaacac ttttgaaaca gagagatttt ttgaaatgaa
     3241 aaaacacaaa gacgactttc atgaacagct ttcaaataaa gtatatgaag ctttagaaaa
     3301 agaaagtttt tttaataatg acaaattagt atgcaatatc ataataaaaa tgtttttttc
     3361 atattcaaca tatcttcata tagaatttga agatttaaaa ccctatattg aaaaattaat
     3421 acattaaggt ggtgtttacc ttgaatcata atactagtat taagaacaag gacagtaatc
     3481 caaaaatatt tttagtactt acactttatc tattaggtat atttatggga gctattgata
     3541 ctggaatagt atcaccagca agaacgatta tacaaaatag ccttggagta aatgaaaaaa
     3601 cgggaatttg gatgattacc atatacacat taacatacgc ttctgtaatt cctatctctg
     3661 gaaaattagc agataaaata ggaagaaaat atgtgtatct agttagtata tttctatttg
     3721 gtagtggttc attaatatgt ggattatcat cactattttc caatttctat attttattaa
     3781 ttggtcgtgt tattcaagca attggtggtg gtggaatcat gccaattgct acagcagaat
     3841 ttggaactac cttcccagaa aacaaaagag gcatggcttt aggtttagtt ggggcaacat
     3901 atggtattgc caatatttta ggttcaagca ttggtagcac aattctaagt atctttggaa
     3961 ctcaaaattg gaagtggcta ttttttgtaa atcttccaat ttgtttaatc atacttattg
     4021 gtggagtttt ctgtattaaa aacaataagt ctgaatctaa agaaaaaata gataagcttg
     4081 gtacattgat gttgatatgt ataatcgtat cactattata tggtcttatg aatatagact
     4141 tttttaattt taaaaattct atacaagata tatcagttta tccatactta ttgacattta
     4201 ttgtacttat acctattttc atatttattg aaaatagagc taaagaccct attttaagct
     4261 ttgaatattt tttaaatccg agaatattaa tagtacttat tttatcttta attgttggta
     4321 ttggaatgat gggtatggta ttcgttccac agtatgctga aaatgcatta aagataaatg
     4381 ctggttctgg gggatatttt gtagctatat tgggattatt tgcaggtatt gctgctcctt
     4441 taagcggaag acttattgac aaatttgggg caaaaaaaat actattacta ggattttctg
     4501 tatctatgat aggctctctt tacctagttt taattgctct aaaaaccaat actgtattta
     4561 gtgtatgtgt aagtttaata tttatgggat taggaatggg atttactatg ggaactcctc
     4621 taaactatat gatgttatca aatacaagat tagaagaatc taattcagca cttgcaacat
     4681 tatccttgat acgctcaatt ggtacttcaa tttctccagc tataatgatt ggatttatag
     4741 cacatgcagg attatctgtg caagataata ttatggactt agttggaaag ccctcaactc
     4801 ctaaaatagt tcaacttgaa gaacttgaaa acatgataga tgacttaaaa tcagaccctg
     4861 aaatggcaaa aaatttaaaa aatgtctcta tcccaaatat gaatgatact tcaaatataa
     4921 atatggacat gacaggtggc aaacttccaa aatccttact taataaagtt cagtcagcag
     4981 atacaaccaa cattaccaat ataacaaaag aaatatcaac aacaatgttt gatgaaaaag
     5041 ttccatctat aattactaaa atacagaaaa atgtacaaaa aggaatagat ggaacacaaa
     5101 gtggaataaa cggtattgaa gatggtgaaa ataaactaaa tagtggtatt aaaggagttc
     5161 aaactggtat tgacaatatg gtaaaagcta gagctggtat taatcaagga atagatggtg
     5221 taaaaaaagg tattgctggt gttgataaag gtataaaagg tatggaacaa ggtcttgaaa
     5281 aacaagatga ggccatcaag gaacttacaa ctgcttataa taaaatacct actacagaaa
     5341 aaactcctac tgataacgaa aataactcaa atgaacaaaa ccagagtaat accagtaatt
     5401 ctgaatcaga cgacactaag aatgattcta cagaaaataa gaataacaat tccagcgaga
     5461 ataaaccaaa tgtaaatgaa caaaaagaaa gtttaaatgc tcaaatacag caactaaaaa
     5521 agacaagaga tgacttaaat acaaagttac aaaaaaataa aactcaaaag aacgaattaa
     5581 gtaaaaaact aagatcaatg gaatctcaaa agaaagagct acaaagcaag ttaaataatt
     5641 ctataagcca aaagaaagac atggaaaaaa gccttgatac aatgcaacag caaaaaaaat
     5701 cactccaagc agttttagca aagacacaag aggtaaaaac tgaaatacca aaggcttttg
     5761 ataaatcaaa attggattat ataaattcca tagaaaataa tagaacaaaa atagaaaaca
     5821 cactacaatc tactctaaac tcaggattta aacagatgta tataacagta ttctgtgtaa
     5881 atctactagc atttataata ttattgttct ataaagaaaa taaaactaga taaaagagtg
     5941 ttatagtatt atattgtaaa tttatcttat tcttaaatat tttattctaa agaataaatt
     6001 cataaaattt ttgtaattga ttaatataaa ttacaatata atactatttt tctcttaatc
     6061 tttttctctt ttacagacaa tcaaaaagca atcttagttt atcgaaataa atttatacaa
     6121 tctattcata caccccttta catttttata acaattaaac ctattgttat atatacaaat
     6181 caaaataaac catatatttt caactataat atgttaaaat catattatga gcatattaat
     6241 ttaagcaaaa tgtcatttag actttaattt agtagtttta tattataaat ttatatattt
     6301 ttaattttaa atgtcattta gacattactc ataaaagaaa ggagtgtatt ttgtggatac
     6361 tttaggaaaa agaattgctt atttgagaaa ctccaaaaaa cttactcagc gcaaacttat
     6421 ggatatttta aaatttgaaa acttaggcaa gtatgaaaca ggagatagaa aacctaattg
     6481 tgatatacta atgtcaatag ctgattattt taatgttacg actgactggc ttctctatgg
     6541 taaagagaag gtaaatgtca atagtagtgt aaaagaagat aaagaagatt acttacatgt
     6601 tactaatgat gaaatgacga tattaaatct atacagacaa ctaaacgaac gtgataaaat
     6661 aaaaatagaa ggaatacttg aacttaagat atctgaatat aaagacttaa aaaagcattc
     6721 ctctaataat aatgaagata aaatggttta aacatacata atttgatacg attttacatc
     6781 atctattaac cttctttttt tgtagtatat tactaaaata tggatattgg attatttaaa
     6841 aacaagtata gtctaatttc aagcacattt ataatattta gattatctta tccatatttt
     6901 agtttaagta ctttgaaaaa tataatacat ctctatgtat aacttagatt cctaagaaat
     6961 gcattactca tgtttttaat tcttccaaca tctttctata cacaattgta catccttcat
     7021 aattcccctc tttaagaagg attgaacatg tactagaata tttagttaaa gtatttaatc
     7081 tttccatatt taagcttttt atattactga gtgctgtaat aaaatcatca attgctctac
     7141 aacactctat aaccccatct gaatccaatt tgcacacaag aatattcacc tactttatat
     7201 actatttatt aatcatcttc tgaatattct atattaagta attgataaat tgatgattta
     7261 tattttttac ctgttcttct tccatttaaa atagctgaca aatagttttc attaacacct
     7321 attttcttcg ctaattccct ttgagtcata tttaattcaa taagtctttg cttgacttgc
     7381 actcccaaaa aaggtatcca tttttttcga gcatccattt atcaacttcc tttctaagcg
     7441 atttatattt acttttatat tattttagct tacgcacccc ctctatttag tatatatttt
     7501 taatatgtac acatattaag tatataaatt tatttaatac taaataagat tttttgatta
     7561 ctttgtgcta aaattaggat gtaaattcgt atcattaagt taattataaa gtcattttga
     7621 ccttaagtca atggttttta tgtcattttg acctttaatt tgaaactatt tacatcttaa
     7681 aaagtctttt tgacattaat tgaatggaga gtgtttatgg atactttagg agaaagaatt
     7741 gtttatttga gaaaagcaaa aaaccttaaa caatatgaac tagaagaaat gttgggctgt
     7801 gacaacttaa gtaaatttga gagaaacatt agaaaaccaa attatgaaat attaaaatct
     7861 atagctgaga tatttaatgt ttcagtagac tggctattaa atggagataa tctatcacat
     7921 aaatcagatt taatttgcga ttcctcctca aactatccac ttaatagcat aaactctaat
     7981 gaaataaagc ttttaaacaa ttttagaaaa ttaaatgatt atgataaagc taaaatagaa
     8041 ggtatgattg aattaaaact tcatgaatat gaaaaagaga aggatttagg aaaaattgag
     8101 tataacaaaa ataaagatga gaaaatagat aaataatttt tatgttgctt ttgatacgat
     8161 tttacatcat ttctttgttc taatattttt atttcattta ttttcttttt tataatttta
     8221 tgataaaata attagtatat tttgctttac ttgaatgtaa ttatttatac aaaagtcaaa
     8281 cataatataa atgtagtaaa tttttagttt caagtaatat ctcgttatta taactaaata
     8341 tttattaaaa ttaggaggat aaaatgggct taagagacaa attcgcgcaa tcatttgcta
     8401 gatcaaaaac tatgagtggc cctgagaaaa aggctaatga aataatgggt aaattgcttc
     8461 ttaagaaagc tatattacct atcgttttaa tgttcgtgat aataatcgct ggagctatgc
     8521 ttaaaatcaa ttcttgggtt acacttggca taaatttagt tattgctgtt ggtgctttct
     8581 tctacataag aaattctagt aaaaagtatc agaactttaa accttatgtt ggcaatttga
     8641 ttagtttaga gaaaaaaggt aaaaaagagt acgttgctat tataaaacaa ggtaagttac
     8701 cagttaagtt acaaatagct tatggtggtg aagaccttga gcatgttaag aaaaatcaaa
     8761 tggttcaaat aagttataat ccagatgcta aaatagctat tttggttaat agacaataat
     8821 ttaataaaaa aaaggagttg tcttaaaatt gattttttct aatcaaattt aaactcaact
     8881 ccttttttaa tttttatata ttatttttat tacttttttt aattaatatt tttaaatatt
     8941 aaatccttct caataaaata gtgaactatc tacaatacta ctttatcttt tcctaacaat
     9001 atataatctg gtgctactct ttcatgttat acaccgtttt gtaacatacc ttttatatca
     9061 gtaacatctt ccttaatttc ctctacatct gttttcatta ttcccatttg aacaagtata
     9121 tttttgttta tttcttgttg ttgtgtagat aattctataa aattttctac agttttttta
     9181 tacatgtctc tatcttcttt tttctcctgc atagtatttt taaataatag agcacatagt
     9241 atacctattg cccctagact actcaattcg gttattaatt tatccattat agcctcctat
     9301 tgtctaaatt ttttatactt atagctttaa tttttgttgt attcttacat catgcaatat
     9361 tatattttca ggattatcaa tttaatcttc tgtactttaa attgtaggtg taatttatag
     9421 attgaataaa taataaatat atatacaatt tctaatataa caaaaaataa gtacaaggct
     9481 tacttattaa agttaagacc ttgtacttta cataaaaaaa ataactagta tatagttatt
     9541 ctaaatttac tattcaactg gtatttctaa ttctttacat ctaattctaa cttgctctct
     9601 aattactgct ggtacttctt ctattgtttt tttgcctctt aaaattaaag ctacatatac
     9661 ttctaccatt ccactctcac ctccagattt tcttacacta aaagtgctta tattacttgc
     9721 ttcattgaca gaactttgat ttaacaacat ctcatatatt tctgcatttg ctataaaatt
     9781 atttgttata tcatcatctt gctttacttg acctatactt atgtctgaga tatctttata
     9841 ctgcatttct acttttctac cttgtttgat ttgttctatt ttatttttat cgaatttaaa
     9901 caattttcac acctccattt taagcttgtg gctcgttgtt ttcagcatca tatctaaaaa
     9961 tcatagatgg acgttttata gctgtccatt tatctccttt ttctactatg attttaggct
    10021 gagcactttt tatgttggct tcttctaatt catagatttc ttggtaactt gagtctgatt
    10081 ttttgatttc aactagtttc atgtctttga ataagtcaga tttatttatt tttacttgaa
    10141 ctttagtttt atttattaat tgatatatag gattgttttc catcatgtca tcctttaatc
    10201 cctcatttaa tgttactatt aaatcagtgt tattttcagt aatgtttatg attgaatata
    10261 ctttattgtt tatcattaaa atagaatcct tttttacttt aagccctgtt tctgatttaa
    10321 atataaacat acttgaatat aaatcgcctg taggactact ggctttaatc agtatcttat
    10381 tttctcctaa tactatttta ttttgtggta tctcaaatat aatgctattt atttccaaat
    10441 tagtcttagt agatattaat tcatcattta taaacatttc aagtttttct ataacattat
    10501 taggatcttg tactacaact ctcacttgag atattgattt ttcaactatt tctatagatg
    10561 ctttacttgt atctactttt gttatcatgt atattgtatc atctgaaagc tctatatcta
    10621 aaatatcatt tagatgttca ggaattggta ttttagtaaa ttcatataaa tcagtaattg
    10681 atatagatat tttatacata gatttatttt taccagttat ccatagacta ttatcatttt
    10741 taattactag tatgtggtca tcattatgtg acttaatttc tttaacatta gttaatactt
    10801 tagtaaaagt tgttattatt ttatcttttt cactttctcc tagtccaaac aatccatttg
    10861 tattataccc ttgagcgtac aactctccct cattagttaa aacaagtgtc attgagttat
    10921 atatacttat atctttaaca gaaggtaatc cagtaacctt ttgtaactct cttacatcat
    10981 tattgtgacc aactcccaat tggtactcag tattctttcc atatgaccat agagtattat
    11041 ctgtcttaat aatataggag tagttatctc cacaagcaac ttttttgaca tctgatatat
    11101 ttacttcagt aaaagttaat aattctatat cttcaagagt ttgattaatt tctttatatg
    11161 aattacttcc tattccatat aattttccat ccttagcaac agccaatgaa tgtgtttttg
    11221 atatatcaat atcagatacc tcaaatggca atattattga agtatactta aaattatctt
    11281 taaatatgtt tttataattt atatacttat tatttgtttt tatatactta tcattaaaca
    11341 ttactagctt ttcatttttc ttattaaaaa ctattttatc tgatgtgtta tctgttttaa
    11401 aatatgaata tagggaacct tcaagagctt catttttatc attatcatat ctatggtgaa
    11461 tacctaaatg attatatctg ttatctcctg ttgaagaata aaattttcca tatatatctt
    11521 taataaatat agtattgtaa ctagaaaata cagtatcaac atctttcttt atagatactt
    11581 tataatatgg aactggtata tccatataat actcatcaga tatgccataa gtatctctac
    11641 ctgatgatat caaattgtta ttattatcta tataaagtgt attattagct gtcacaaaaa
    11701 catctttaac atcttccaca ctatttgtat tgttattatt tgttatactt ccatatccac
    11761 taagacctcc aacaaatagt ttattgtcat ttgttaaaat ggctgaagta tatccattaa
    11821 ctactatttt ttttgcattt ataatattat taattactcg tatacccttt atatcattag
    11881 tttctgttga aaagaatacc ctctcttttt tccctgtagt ataaatcata ttgtcatttg
    11941 ttataaaaaa tgtagcttca cttcctgctg tcacaacaga ttttacattc tcaatattta
    12001 tttttgtaaa tgtacttata ctactttcag tgaatccaaa atatccattt acaaatccac
    12061 atacataaca tgtattatca tttttgacaa tgattgatac atcattacat agatatacat
    12121 ctctaacacc atttataggt atacgtgtaa atatattcct acttgtagta tcacccaatc
    12181 ctaactgtcc ttttccatta ttacctgctc catataaaag accatcatta gttaataata
    12241 gagatgtgtt tccatataca gttacatatt ttacattttc aacatttatt ttagtaaata
    12301 catttctatt tacattatct cctattccta actgaccaga gttatttaaa ccaacagcat
    12361 aacaggtatt atcatttttt atagcaaatg catgtttact gttgtcagta aatctattta
    12421 cacttataga ttttatatta tcaatattta ttttagcaaa tacctttcta tttatctcat
    12481 ctcccactcc taattgacct acatcattta aaccaacact gtaaagcata ttattattag
    12541 ttaataaata tgtagacttt gaatggctat atatttcttg tatattagaa gatatttcac
    12601 tactgctatt ctttttattg taatttatct tattatttaa atttaaacta ctattaactg
    12661 atataccatg cgtaccctta gagttatatc cacatataaa taagctctta tcagttaata
    12721 ttgcaaatct agttttatct ctacttaaat cataaggagg tataaatata ttttttatat
    12781 ttttcattac ctttttatag ttagttgcat tatatccacc ttgtaagtct gatacatcta
    12841 gagcttttgc actttcagat atattcccaa aaccatatag tgtattagag gatagatata
    12901 ataatgcttt atataaaagt atagctttgt cagaggtaat aggacctgaa cttatattag
    12961 atgatgcaat attactagat gatagattat taaagtaatt ccttccatat tgatgtatat
    13021 aagatgaacc tccagaaata agtaaattat ctggattgac attttttaaa ttacttgatg
    13081 attccccatt atataaataa gcaaaagttg tagaagatga tgattatgta catcttgtag
    13141 gataataatt atatatataa gaatcaagat atcctgcata tgaaattccc tgatattgaa
    13201 ctctatagct tgaatcagtt ggttttgtcc ataaagtacc atgatatcca gttagaaaag
    13261 atccaatgtt tgtacaaaat atagttacat actcatatat gacaatatta ttaattttag
    13321 taatatcatt tgtgcatgaa actctactat aacttgttcc tatagagttt atataattcc
    13381 atgtacttaa taagtttaaa tttgcagtat atgcttcatt gtttacaccc actcctagca
    13441 tatagttttt atttatacca gttacatata cctttttctc atcaaccaca ataaatgttg
    13501 cgttgtatgc actatatgca tctgttacat tgctaatact aatctcagta tattgacttg
    13561 ctgatttatt aataagtcca ttaacataac ttgaactctt tccagaagta tatatttgct
    13621 tattattagt tacaattaat gaaaaccaat ttgataaatc agtatttata acaacttttt
    13681 ttacattatc tatagatatt ttcttaaata tatctgaatt agaaggaaga acacctgcta
    13741 ttgagttact atttggtcca gaataataac aagtatcatc atttttaatg tagaatattc
    13801 ctccagaaat attttttgat gctactatag actttacatt agagatactt aattttgtca
    13861 attcatttaa ttgacttggt aagtcagaac ccattccttt atatccatta tttcctagac
    13921 catatacatc tccattccct aaaataacca tagtacaata aggactaaaa acgacatctc
    13981 ttacagaatg tatatctagt ggaagttcaa tatcctttat ttccatttct tcttttaata
    14041 aagttaattt atcctcagat ttaattaaat ttatataatt actataacta tatttttcac
    14101 tatccatttt cttaaattct acaatgtgca tatcatcaaa aaacgaacct aattgtccat
    14161 aagtattatc tcctgtacaa tatgaatcat tatttgaatc aattagtatg ctatgtgata
    14221 aaccaatgaa aatattctta gctacaatat tttttaaatc tataaatgaa ctatctatat
    14281 tctcagagta atttgctata cccaactggt atatatcatt atttccagca accttcaaaa
    14341 tacctttatt atctataagc atagtattac cattaccaca tgcaatagtt tttatattta
    14401 cttcttctat cttagtaaat agtgatgatt gaagtgcatt tccaaaacat ttttcctttc
    14461 cacaaccata tatatcatta ttatacgtca ttaacacagt gtgttcagaa ccacaagcta
    14521 tagtcttaac atcattaata gaaactaaac taaataaatc tctattattc ttatctccta
    14581 aacctagttg accttgatta ttaaaaccag tagaatagca tttattgtca ttctttatta
    14641 taaaagctga tttattatat gaaaatacag cctttacatt gtcaacatct acttttgtaa
    14701 atgtatttac atttgcagat gctccattag ttcctagacc taattgacca tacaaattat
    14761 ttccagcaac atataaagtt ccatcattgt ataaaagata tgttgattga agtccacatg
    14821 caatatcctt tacgcctttt atttctggaa ccttctcaaa tgtagaaact gttttcccta
    14881 ctttttctaa tcccaatccc atttgaccat ctgtatttat acctgcgaca agtaattctc
    14941 catctgaagt aagtataact gcatgagatt ctccacatgc aactttttta atattggtag
    15001 atatatttgg tattttcgta aataattttc ttgttgtatt gtcttctccc aatccaagtt
    15061 gaaaattact attatctcca catccataca agatattatt attttttaga ccaaatgtat
    15121 agttacaaca tccagtaaaa aaattaataa agtcataatt atcataccct tcatcaaatt
    15181 catcaactac catattttta tcgccaaaga aattacctct ttgaagtagt ttagttcttt
    15241 tcattataaa aacctcctaa ttatcttatt tcatacgaat atttatttaa agttatatct
    15301 gtctgaccaa ttgaatatgc cctattagat tttgtttcaa caaagttatt atccatttca
    15361 tgtatatatc taactctatt tgcattaaat ggtagcacca cattcttaaa tgtaacacta
    15421 ccaagttctc cagttataca ctttctattt aaatctaact taaggccttc tatagctatt
    15481 atgtttttag cattagttaa ggtatcatac caatatccag cttcatctac atctatttct
    15541 gactctaatt ctaagtatat taagatatct aatacttttt cagataattt attgtaacta
    15601 ttttttaata cagatagttc agtttgtatt tgacctccta ttacatcatc aatagatata
    15661 tttttaattt tttctactac ctcatttata gctcctgata agtttttagc ttctgtagat
    15721 aagagttctt ctccaatttt tacttttatt tcttttatat ttttatcaaa cttatcatct
    15781 aaatcttgaa tatccttttg agttacaaaa accaacgtcg ggtctacttc taatgtaaca
    15841 tttgaagtat ttgacaattg taaaatcatt tttacaacca aatctttaat actgccatct
    15901 tcagcacgtg gcttatacgt ctctggatac ttacctatag ctattatatt attctcatta
    15961 tccagaacag cagcttctct tatcataaat ccacctactg ttcctggtaa aatagtctct
    16021 ataactatcc aattaggatt tttttcatca attcttagag aatttatatt tccttcccaa
    16081 actacattct ttaaagcagt ttgttcctct gttggctcat aatattctcc attactatct
    16141 ccaacttgaa acttggttaa tccaacaagc tctcctagtg cagttgcatt tgctattttt
    16201 tctctaccaa ttttggttaa tattatataa aatttttctt ccaattatac cacctccgat
    16261 ttaggataaa ctactacatt ttctacacct gtatcatttg caatagctat attaatctta
    16321 caattatttt caatgcattt aatgcttaaa ggataaacct ttatttcttc tctacttaac
    16381 attccagctc cacagtttac aacagcacta cattttataa ttggttcaaa gtatggatga
    16441 acttttacct cttcactaca taccatacct ccaccacaat agaccatact tttatctagt
    16501 attattggtt ctaaatagtg taataagtga gcaggtttta ctctttcaat catatcgcta
    16561 caatctaaaa gagttttata gtcacagtta tttgctataa aactcaatac gaatgtaaat
    16621 tcatcactat aaacttttat atctgtttct gattttgtat atgcctcaca tatacttttt
    16681 ataacttcaa tagtactagt acctctgctt ctcattttag cttttatatt gctacgtctt
    16741 gtgtcaaaat caagttcttt tttttcaatg cagagtatgt cttcccacat atctaatccc
    16801 catgttgcag tgtcaacaaa taattggtta aaggtatcat caatatcttc tttaagaata
    16861 tttaattctg tatcgtatgc accttgtatc tcctctacaa tgtaatttct atcaaatgat
    16921 ggtagtttat caattagctt cattgatttt ccacctcact aaaattaaca gttgttacac
    16981 taggtatttt atcttcatta atagttatat tatctgtact tccatttata agtaggttac
    17041 ttaaatcgtg tacaccagta gtatttataa gtattcccat tacttttgta tagattatct
    17101 ctcctctaat atctctaaag tatgtattta tactttctag gaaagattct tttacattgt
    17161 ctaatgtata tccatcttct agtttcatta ctgcacttat acttatttct attggtaatg
    17221 gtgtcacaac tgttatagtt ggtccaatag gcttctcttc atctatatgt tgctgacacc
    17281 tttcaattgt ttctgtatca acagcttgat tatttttccc aaatatcaag accttaactg
    17341 ttcccggacc atcccatctt ggataaacct taacattata gactccatct acttccaaag
    17401 cccattcttc atagtgagct ttatttccac ttgtagcttg atttctttgg attttataaa
    17461 atctttcttt tagttcttca tctgtctcta tatctgtacc accttcaaaa cttcttgtgt
    17521 tagtaatctt tgttactcca gatatattat caactagttt aaattcacaa tttgtaggta
    17581 aattatattt cttaccaact tccagagctt gaactgggct attgtcacct tcttcactac
    17641 caatagttac atcttttatt actacaaata gtaaatctct atatgatatt attgtgccat
    17701 tatttattac agtacctttc tctccaataa attcaacttc tccatttgac tctgtaccta
    17761 actttctata tacaccaaat tcattgactc ttttatcaag aaactggtta tatgtgtctt
    17821 gtataaaagc cattttatgc atctttgaaa gttctagata tatctttgaa agttctagat
    17881 tatttccaga taccatgttg tttagaaaag aaccttctcc tttataaata tcaagattaa
    17941 tattttcaag agttctattt tttattactt catatgtctg gtcactatac atttatttcc
    18001 acctccccat agatagtgga tacttttaca tttgcactca atacgtctct gttaaagtta
    18061 gcacttttta cgtttacttc taatatatat ggatttatta gaagggcctc ttttatgaat
    18121 ctactagctt cactttctgt aagacctttt gtatattttt gtcctattag ttctgaaagc
    18181 tctgtcccat attctaaaga gtatatctca tgttcatatc tatttgtctt tatacacttg
    18241 tatatccaaa cttttaaggc ttcttttttt tcaattattt taaagtcacc tttttctaaa
    18301 ataggttcat ctttttcaaa atcccatgcc acttcacgaa agattggcaa ttcttctgtt
    18361 ttaggtaaga tataatcctc tgggacacct ataaaaggaa atattgtact cattataagc
    18421 tcaccaactt acttacaaca gcaaattttt cgcctattct aaacattatt accatatccc
    18481 cagattcaaa attatctata aaaggatttt ttacttcatg ctggtgttct tgatttgttt
    18541 ctgtgtcaaa tgtttctagc tgtctatcaa tcatccaact atctatcaaa atatcttctt
    18601 tttctaatat gatgttattt gtctctattt ttaaatctgg taatttactt ttaatttttc
    18661 caataaaaaa agaaggttga ttatgatact ttccttcttc tcttattatt cctataaatt
    18721 catttattgg attagccatt atatcaccat ccttttataa tactctccta gctgtattaa
    18781 aatctcttct actacttaac ttacttattt taactacatc acctttttgt ggagcatgta
    18841 aaaattctcc attacctata tataatccaa catggctgac tggattatga aaaaatacta
    18901 aatctcctgc ttgaagattt ttttgttcta ctttcttgcc ttttttagat tgttgatttg
    18961 acgttcttgg caaactaaca ttaacttttt taaaacaata gaccattaaa ccagaacagt
    19021 caaaactact tggtccattt ccaccccatt tataaggttt acctagatgt ttttttgctt
    19081 ctgatattac tattttagct ttattagatg cattaccaga ataaccaccg ataataattt
    19141 ttccttttct ttttccaaat cttttcattt cttctgcatt ttttactaaa acatcgatat
    19201 gataagttcc atctttttct ataattatag cagaaccatt atcatttact ttatacagtc
    19261 catcacgtcc agaaactcct gttacaatac ttacttcatc accatattta aataatgggt
    19321 gttttgataa gaactctttt gtataataag gtttttcata agctccaacc ataggtgcag
    19381 cacatgtctt tttagaagga ttaagctttt tctctctaca gtctgtatct ccaccttcta
    19441 taccaggtcc aggataatat gctgtaaata tagcagaaaa ttctgtccct cctgcataat
    19501 cttctccccc taaattactt tcttccttag gttcatcctg tcctgctgac ttttcatcca
    19561 taaggttttg aaaattaagt tcaagctcaa tttgatactc tccattttgc caagtatgtt
    19621 tgtctgtatc tatataaaat agtcctacaa gctttgtata agaatcttta acttttactc
    19681 ctctaccagt tatacaactt acatctccat aacctttaag agaacagctt ttttctatcc
    19741 cattaaactc gctatcaata tctacatctt gattttcttg ttgctgaatt actttttgca
    19801 ttattacatt tacttcctta aaaatttcat tatctatttt ttcgctaatc ttgcttccat
    19861 actggtctac cactattact ttatttttta cattttccat gctctctgaa aaggtggtat
    19921 ttataatatt aaatccctct tcaaacataa cacttaatgt aacagttcct ttttcaataa
    19981 cattaaactt atctaaattg gcctctatca tatacttttt ctttgtcttt ttacttgctt
    20041 ctgtatatgc actcattatt gtgtcataac catttactcc tataaacatc tttgtatact
    20101 tgacattggt ctttgctatt gttccaactg aaagcctatt ttcagcaaat acttgctttg
    20161 ctatgtcttc aactaactta tctttaaaat tgtatgacac ttcactttgt gtaagtaaaa
    20221 atcccatatc tttagataca aaactaattt cattactgct tgaatcttta gacctattaa
    20281 ttatcattcc tctaaagagt tctttctcat ctacatagaa acagactgta ctagctattg
    20341 gtatatcgat ttgttggaaa tttacatcaa atgatgattg tattattgaa aactctagtg
    20401 tccttgatgg agatttataa tcacctgacc atgatacctt gtctactatg tcagttatgt
    20461 catatatact tccgtttttt atgtgtaccc aaatcttaat aataaaaaat cacctgcctt
    20521 ttcatttaat tctatcagca gttatttata acttattatc tataatagtt attgttttgg
    20581 agttactttt ggtatcttta ttcttttata ctcttttaaa tctaatgtaa aatatacatc
    20641 tcgagtccca tctctttctt cataactaaa cccttcaatt atgacttcca tatttatatt
    20701 tgtttccgta attgtaaatc ttaatataaa accttccttc atccattttt ctatcttatt
    20761 tacacaatca tatggtgatg gaaaacctgt atagtcacag aaagtatagt ctttatttgg
    20821 aaaaaaactt gatattgaaa tgcttttaag agctacacca ccaaaagttg ctatttcacc
    20881 tattttgagt atactagaag agtttacagc agcttttcca ttaatactaa aggaagatgg
    20941 aaatactgga aatctaaatc tatcttcagc ttgtctaagc cacatttcca ttataagaca
    21001 ccccctaact gtgccaattg gatacttgca actaattttg atgttatctt ttctatatca
    21061 gcttcttctc taatcactat tgtatcagct aacttagcta tgtttatatt tccaccattt
    21121 tttctgcttc tatattgatt tgcttcttgt ttagttaaaa ccatttcacc ttcatggagt
    21181 ctggtttgat agtcattata aggaacatag ttcaatccaa atgcattatt acctttctta
    21241 cctgaataag ctttaccaga atcaccagcc tttgttccag catatggatt atctttgtaa
    21301 ttttttttac tcttttccac tagatttaaa actgttcctg tgatgcttcc attttttatc
    21361 ttattaatca tagtttgcca ccacttgcat atatctttaa ctattttaca tactttatct
    21421 aatatgttaa aaaaagctcc caatattggc tctataattt tccatccagt ttccaacaga
    21481 ggtcctaagg tctcccatac agttttccaa ataacaccca gtgtctctat aaaattgttt
    21541 atttgaggtg catattcacc taaaaaagta aaaattcttg tgataatagg actaacagct
    21601 tcaactacag gtccgattag ttggaatgct gtagttacta ttgtaccaaa tatacttatt
    21661 aatccttgtg caattggtga tttattgttt atactatcta gtaaattatt tactaagttt
    21721 actactggaa gtaatgaatc tattaatgct gtaaaatcga tattttgtaa tagtgtactt
    21781 cctgcgttaa caattgcttt tggtgcattc cctatcattg ttgctgcatg attttcaaaa
    21841 cgttcttgct tattttcaac catcatttta cgcttttcag cctcattaga aattcgatat
    21901 tgagtagaat cagaaaactc ttttccattt tttacacttc tagattgcat ctggtcatat
    21961 ttgttgccaa taattatctt accattcatt acattgccta ttttaccagc cattttttca
    22021 gataagccag ctaaattgcc taatccactt tctataaaag tttttatttt ctttagtcct
    22081 tgacctaaaa tatcatcaaa aggtttaaca atactcttca agccattgtt aatgcttgtt
    22141 tgagctcctg atattatacc tggtattgta ttactcattt ctttagctaa ccccatctgc
    22201 tttacactta tgccttccaa tgcttcatca aaagttttat aattttctcc taccagagta
    22261 ttaagagatt ccatgtttcc ttttgctgca cttaagaaag ctgctgatac atcttgttca
    22321 ctacttgtat tcatatttaa agctctaaca tctaccatag cttgagttat atctttttct
    22381 tttttgccac cagtaatatt actaatcatt ttagctttag ttcccattgc aacaacatct
    22441 tcagggtcaa aaggcgtatc atttgccatt cttactaaac tgccataata atcatcaaca
    22501 tctatttgat taacttttcc agtatttctt ttcattacac tttctagaac aactttctga
    22561 ttttgttcct gaccaagtaa atcaacaccc tttttcacag cgacacctat agtagcacca
    22621 tttgtcaaat cagatatctt cccttttatc gaaccaagta ttccacttgc catatccttt
    22681 acagctattg caccactcca taccttacct gcaaataatc tcaatttggg aaatacttta
    22741 cttataatgc cacttgccat atcttttata gctattacac cactccaaaa agtattttta
    22801 aacagcatta acttaggcaa aattctaccc atagcactac tcaccatatc tttaagatta
    22861 atacttcctt gataaggacg tttctctact tgttttaatt ttcccaagat actacttatc
    22921 actctcttag tcatatcttt tgcttttatt acgatattcc aaatcttgcc accaatcttt
    22981 tttaaatttc caagtaattt atttttagtt ttttctactt catcactttt agctttaaat
    23041 attaatataa tcataatatt tgaattaaac tcatttttct tttgaatcaa cttattaaaa
    23101 taatcaataa tcatagctat agttattgct agtctcataa tacttccatt taaacttcca
    23161 ataactcctg aaacagcatt tatgctagaa cttaattttg tcatagttga acttacacta
    23221 ctagcagtag tttgaacttt tcttaacgag gaatttaaac tgtttactct attacttgca
    23281 cttccaatag aattttttat gctactagcc attacattag tggaaagtga aacagccaca
    23341 gttgctctcc taaaattatc agttactgat ataatttgat tcatttttgg tgtgtaatta
    23401 tctgtaaggt acatatttat accttcttct ctcacatttc ccattacata ccacctcctg
    23461 tcaaaggaca cactccatat ccatgtttca ttttttcaac ttcttcctca atttctaagg
    23521 cgaaaaaagc ttcaataagc tttaattcgc ctttatccat tgcataaaaa agggacggtc
    23581 ttataccttt tttcttccaa taatagtaca ttattgtagt cctggtatcc gtccctatta
    23641 gttttttacc tcttctatca ctgcattttt accataacac ataacatctt gaagtattct
    23701 atataagttg gtaatttcac ctggtaataa caattttctt gctaattctt ttggtgttgg
    23761 agccttaaat ttatccatta gctccttatt tttaaataaa agctttccat ccaaatcaca
    23821 aacaccttct ataattgtaa gcatctgcat ctccggtaag tctatatccg ttttcatatt
    23881 ttcatcaatt ttaagacaag tattttgtat ctcttcatct ttacttattg ttaaagctct
    23941 acacattact ttaaattcct gcccaaatat agttgataat ctcttaagtt caactatagt
    24001 acttggtctt tctaagtttt ctgtatctgc atttaaaagt aaatctacta tgtttatatt
    24061 ttttaataat ccattttcac tcattattat ttgctccttt atcaatcaaa ttcaatcaac
    24121 taactttttt acttattaac tatctgaatt tattaagtat atatttacta aatatcagta
    24181 taaaaccaaa tataaaacta aatataaaac taaattatat caagaaaatc atactcagta
    24241 aatgtgaaag gtgcttctat ctctcctttt acgccaacct cccaatcagc taaagttaaa
    24301 tcatcaaatg ctatattctt tactgctatt ctttctgctc cataagaatc tgggtcattt
    24361 aatttagata tagcaacaaa tctaggttca gaacctctct ttatcttttc tccaattaac
    24421 ttgtgcattc ttgaactaac atggtataga gttattgagc cttttccttt atatcccata
    24481 tactttgtat cagtacccat ttgacctgct attataatat cttcttttgt aaattccatc
    24541 tttgcttgaa acttctttac ttctgctact ttgtttccat caagccaaag ttctccccaa
    24601 gtaccactca ttacatttct agcttccata tttgccatat taataatcct cctatatttc
    24661 tattgataaa tctatatctt ccatagcatc aagtactttt atttttgctt ttaaaaatac
    24721 tttagaacct gtgttagctt cttttatttc ttgtaatgtc atataagata aatctactcc
    24781 ttttgatttt aaatacgatt tttgtgcttc aaaatctatt tcaacagtag aatcagattc
    24841 tataagtgct gatttttcta attcttctaa ataacttttt atagctacta tcaataaaca
    24901 tttgttgtca taactgttag taacctttcc tatatagtca tctattatca cctttcttat
    24961 gtcactatgt ataatatcta aagtgtcaac tatttttatt ttctggaaca tttctccttt
    25021 ttctgctgtt aattcagtta aagaatttac tcctctagca attcttatag ctcctgcttc
    25081 tttaataagt ataagctctc ctttattaac ctttgattct gcatcaactt tcgtcatctt
    25141 aggtatatcg actacatcac taagtttagt ataagttact gattgactta aaggtgtacc
    25201 tgctataagt ccagccaccc tacttgtaaa ctcatcaaca ctgtatttct tttctccaac
    25261 taacacatct tctgtagtaa aattaattat cccttcatga tttcctacaa cttttcctaa
    25321 tacagcttta accttaacct tatctatatc tctaagttta attatccaat tttttatagc
    25381 agtcttatca gcttctactg cttttggcat acatagataa ttaaattcct tagtctctaa
    25441 aaaatctaat gcagtttgaa tatctgcttc tccttctatt acataaacta ataatttatt
    25501 tggagtgtta acatttccca tcaaggctaa atttatatat tctttatttt cagcagataa
    25561 atcaactggt atatcctctt tttcatgtat ttcattaaga cctagtgcct tagcatcttt
    25621 aagcaccatt gcaattattc ctctagctga acgttcttta acagttgtag ctagctcctt
    25681 aaatgatatg ttgatacttg gtaatcctat agccatacta tcactctcct aaatttaaat
    25741 tgatttcttc catattttca tatactacat cttcttcata tacactatcc agatagttta
    25801 gtgtaagtgt aaagttcaat acataaattg actcttcttt tttaatactt tgttctagct
    25861 ttgttatatt taattttctg tctgttacct ttatatttct gttaaatagc ttatttaatt
    25921 catctgaaac ttcatacatt ctaatactct ttgactgtgg ttttggtaag tattttatag
    25981 agataacaat atctttgtta ttcgtattta acattacagg tgtacaaata actgatagta
    26041 tacttacaaa aaaacaagac ccttcagagc cttgcacact ttcatcatca ataaaaatac
    26101 tttcagtaaa gttttttttg agaatctcaa taattgtttc taatatttcc ttgtatttaa
    26161 gcaaataatc accacactat cactaaatat tcttgcttaa tctgataatc ttactataaa
    26221 tgatataagt catttttaaa tatatatctc atttattcct gtcaagtatc ttctatattt
    26281 ttaggtaata ttattctttt caatctatca cctccttcaa tatggatttc tttcacaata
    26341 acattatctc acatatttta ttgcctattg ttgcaatggt gtatataaag tgttgtaaaa
    26401 ttgtcctatt ttttataata tctcactcaa tttaaatatc atctttttct ttaaattgta
    26461 gcaagatgtt ctatctaagt gtagtttcat agaaatatat gtcatattgt tttttgtctt
    26521 actattataa aaaagattaa aaaattccat ttctatatca tttagacacg ttagagcatt
    26581 ttctatcttc tctttttcta tttctatatc ttctttttcc atttttaatt ttcttaatct
    26641 ttcctctctt tttaacacct cattttccac agaagagctt atgttatatg tgttactagt
    26701 cttttctgta tataccattg ctccacatcc agagtattca ttctcacatc tttttatgtc
    26761 caagttaata ttctttatct ttatatccaa atacttataa ttatgtaatc ttttatctgt
    26821 ttctttaaat aatatatctt ttttgttttc catagtactt ctcctttttt atatacttat
    26881 tattagcttc aaatttacaa taatctcttt agtgcacagt tttttactaa tctcacaact
    26941 ttcaagaaca tattgttcta tgactgtata aaaaaaactt cttctatatt ttcttctaaa
    27001 atactagaaa tcttcttagc cataagaagt ccaggatttt ttatgcctct ttctatttga
    27061 gatatataat catttgtaat tccagccttt ctggcaagtt gaatttgtgt catacctttg
    27121 cattttctat aatatttcaa gttattttta aacattgttt tatacctcca ttactattaa
    27181 gaatattttg ttcttcctca cttagattat atagaattat ttgttcttta tcaatatatt
    27241 ttagaaaaaa atattctttt atattttatt agttttattt tttagaacaa tttgttataa
    27301 tctatacata aggtggtgtt ataatgtttg ccaaaagatt aagagaactt agaaaggaat
    27361 ttggattgac tcaaagagaa cttggagaaa aagtaggcgt ttcccaaaga gtcttagggt
    27421 actatgaaac agaaaacaga tttcctgatg aacacatttt aaataaattg gctgatgtat
    27481 ttaatgtatc agttgattac cttcttggaa gaaccttagt taaggaaaat attgatacag
    27541 tagctgcaca tagaaaaaat ccacatgaag agttacctga agaagcacaa gaacaactta
    27601 atgattatat agaattttta ctaaataaat ataaaaaaaa ataaaccata tgatgagcag
    27661 tctatttttc tgctctttta atataattta aaggcaaaca tacattctat tttaggggga
    27721 tttttatgaa taatttggat aagctttttg aattagcttc tcaagaaggg ataataattc
    27781 attataccac ttatattgca ggtgatttag aaggactata tataaacaaa catggtatta
    27841 aaattatatc attactcagt aatttaaaac aaaactcaaa aaaattaaca tccatcttag
    27901 ctgaagaact tggacatcat ttcactagcc ttggatacta tgtatcctct tataacgatt
    27961 attatacaaa aattattata gataagtgcg aaaataaagc cttaaaatgg gcttgtgagt
    28021 tcttaattac tgaagaagat ataataaaca taattaactc tggcatcacc tgcgtttatg
    28081 aaatggcaga catacttaat gtggatatta ctttctttca aaaaagatta gaatttttat
    28141 cattaaaaaa acagtcttta caacttggaa ataataaata tttaatatta actaatttgc
    28201 cgtatttcta catatttgat ccaatttcat aataaaaata gtagtaattt taactggctt
    28261 aacagtaaaa tctatatatt tattttaaca aattttggtt tctataaatt caatattaat
    28321 atataataat gatatcatat tatttaaaat tctaaaaaca aatatatgct tttgttaaga
    28381 aaggttgttt gatgaatgta taaagaaaag ttgtttgata attattttaa gttcttagca
    28441 ctattatttt ggccaattat gtggtataag tggatagtaa tttcaaatgg tactcttgaa
    28501 aatatgttat ttaccatata tgcaatcgtc gcaattgttt tcatcatttt atattcggtg
    28561 tttatgataa aatataaaga cattacacaa atagatttct tttacaggat aagtactcta
    28621 cttgctttta tatttacact ttttagcttt ttaatatacc caaagagttt atttttcttg
    28681 tatttaaaga ttatttttac tggaatatac ctttattatt caatagtaaa aactcttaaa
    28741 tttaaagatg acgaaggtgt agtgggtatt atgagctcac ttttattaat tgtaataact
    28801 ttgttctact aaatttaatt ctatttgact taatatggct ttaaatatta gacatctaca
    28861 ttattttctg atattattta agatggtagt cttatattaa taatgaaata cactatgaag
    28921 gagttaacta ttatggaaaa aaaagtttta gcaacagttg gagaaaaaga gataactaat
    28981 attgatattg aaaatgcatt aaaatcatta gacccttatc aagctatgca atttaaaact
    29041 gaagaaggaa aaaaacactt attaaatgat ttagtgaatc aagaattatt cttcttagat
    29101 gctaaagaag aaaaattaga tgaagaagaa atatttaaat tagaaatgaa aaagatagaa
    29161 gaaaatgtat taaagcaatt tgctataaat aaagttttat ctagtgttaa tgtaacagaa
    29221 gatgaaaaag taaaattctt tgaagctaat aagagctctt tctctaaacc agaaagtgca
    29281 actgctaaac atatattagt agatagtgat gaaaaagcta aagaaatact tgctcaaata
    29341 aaatcagaag aaatatcttt tgaagatgca gctcttaaac attcttcttg cccatctaaa
    29401 gatatgggtg gtgatttagg aacattcggt agaggacaaa tggttcctga atttgaagaa
    29461 gctgtgttct ctatggctaa aggtgaagta agtgaaccag ttaaaactca atttggatac
    29521 catataataa aattagaaga ccttcaagaa agtacagagt ctacatttga tgaagttaag
    29581 gctgaagttg aaaaaagttt attatatcaa aaacaaaatg aagtttatgg aaataagata
    29641 aatgctctta atgctaagta cggaaacctt gtaaaataca acgattaatc ataaggtcat
    29701 atataaaaaa gctgtctttt cacaatattt attagaaaag acagcttttt atattaaagt
    29761 ttaaatattc agtttcatat tatcattctt ttattgtaaa gaactcagct taaaagttta
    29821 aataagattt ttaattatca ttagctaaat ttttatatcc acgtattatc tcataacagt
    29881 aacctattac atctcttctt tttattatcc caataaatgt gtcttggtca tctataacag
    29941 gaatgaaatt ttgatttaaa gattttataa ctaaatcttc aatatctgcg ttgatggaaa
    30001 caggagaatt atccattttt ctatttatat ctgtcactgg aacatcttcc aatcccttta
    30061 agtccagatt taaatcattt tttaatgtcc ataaaaaatc accttctgtt atagttccaa
    30121 catatttacc atccttactt attattggaa ttgcagagta tttgtgatat tccatttttt
    30181 ctagggcttg ccttatagtg tagtcttcat atatatatgc tacttcactt tttggtgtta
    30241 gaaagaataa tatgttcatt acactaccca ccgttactga aaacttataa atattactca
    30301 atatttataa tgttactttt ttgcagtaac tttctccttt cttattttct aagtattctt
    30361 aatactatac gctgtaacaa gcgtttttcc tttgctcatt ttaatcttat tatgctttat
    30421 taatattttt atatggtcta tcagtatcac ttattctctt tttagaaaat ttactaaaaa
    30481 ttaattgact taaacttagc cataataatt ttactctaca ctattaattt taacatattt
    30541 atctcattat taatgatttt attttaataa ttttaattta ttttgctatt aattttattt
    30601 acaaaaactg aaaaaaccgc taaatgatta gcggcttatc tctcatatta ctagaacact
    30661 aatatatatg aatttttaat tataatctaa ctacgttagc agcttgagga cctttagctc
    30721 catctactac ttcgaactca acgctttgtc cttcttctaa tgttttgtat ccatctccag
    30781 ttatagctga gaaatgtacg aaaacatctc cttcaccttc tacagatatg aatccaaatc
    30841 ctttttcgtt gttaaaccat ttaactacac cagttttcat taataaatcc tcctaaaaaa
    30901 acacaaatat ttagctactc ttttctgtaa ccttattact actctatcat atatagctat
    30961 gtttgtcaat tattattatt tttatacatt aataatcaat ttttttactt tataatttta
    31021 aatgattatt atcttttaca ctttattttt ctttataatt ttttaataat ttatttttat
    31081 tatatggaat cttatggact tatcactcca gcctaaatcc catggcacca acattctatc
    31141 agtattatgg caagttacca aaggataccc tcttgagtct aatcctgtca ctgtcgaaac
    31201 atgtgttatt ctgcctttct tctcataacc aacaaaatcc cctggacgta aattataagc
    31261 ttctttgtat acttcttcat aggaaccttt agcaattaat gaacctcttc cactatacag
    31321 tgcataattt ttaaaagctt gtgcatttac ccatgctttt gtacctgctc tctcgctata
    31381 attccaaatt gagttctttt tgaaccttcc actttcaaac attatctgtg atgcaaagtt
    31441 tgcacaatct ccaccttctg gattataatc cctatagttt ggattaaatt tcataccatg
    31501 ttcttcatca gctgcggctc cacagtattt atgtgcataa tctatagcct ttttttgttc
    31561 atctgtaagc tgtaactcta caccatcttg ttgaccaata tagtttctta tatcatcaga
    31621 ttttatattt tctaaattta aagagtctgc aaaagggtct gtataccact ctttagttat
    31681 gacatattga ttatctataa tttttacatt tagataatga tatgtaccta ttctaaaggc
    31741 attttctttg tccttttgat tttcatatga atacttatat tcagtcgata ctgaacagat
    31801 tataccatag aggtcttttt ctttttcttt tattttttta atcttaaccc ttgtctttat
    31861 gtctttaaat tttacacctt gcttactaga ccagttttct aaatacttca tcttttttac
    31921 ttcattttca taagcccaca gaccagattt tttatccgtc tcataaagac cttttaaaat
    31981 gttttcattt ttctctaata ttgccttatt tctgtagtca aataagtttt caagcaatag
    32041 tttatattgg tcttttacat catcaccttc aacatttgca gattccatga ctttttcttc
    32101 catatttttc atacttaaat cattgattaa aaatacacct gctgtagtag ctaaagacaa
    32161 aaatacaaat gccattatac ctttgatgta cttattctta tttttcagta ttttcaaagc
    32221 tacacctccc aaatagtaca ccttacaaat caaattacaa agaatattct gtcacatata
    32281 gtaattttca tatttaataa ttctctattt tattatgtcc aatattaaaa attaattgta
    32341 tcaatacact gtttttttta caatttttta ttttataatc tacttttttc ttttttttat
    32401 agtaatatat atgtatataa atattttggt atcattataa ttaatacata ctaatggagg
    32461 gttttttatg caaaaaaaag tcgcagcaat taatgactta tctggaatag gaaaatgctc
    32521 attatcggtt gctataccta tactttcagc attaaaggtt caatgttgtc catttccaac
    32581 ggccatactt tcaagtcaaa ctggttatcc tgaatttact ttcttagact ttactgatga
    32641 aatggtaaaa tattcaaatg tttggaaaaa tttaaaagtc aattttgata gtatatatag
    32701 tggttttttg ggttcaaagc atcaaattga gatagtggct aattttataa acgattatcc
    32761 caatgctttt atcgtagttg accctgtgat gggtgataat ggagttatgt atccaatatt
    32821 cacagaagaa atgagacagg aaattaaaga attagttaaa cattcagatt taactactcc
    32881 taacttaaca gaggcttgtt tcttgactgg aaatgattat actaaaagcg attataatag
    32941 agatgagctt atatatattg caaaatctgt atctgatttg ggacctagca aagtcgtgat
    33001 tactggtata ttagaagatg ataatatatt aaatttggct tacgatagag ataatgacca
    33061 tgtattcttt acatcagtca aatataataa ttgttcttac agtggaactg gagatatatt
    33121 cacatctata ctttgtggta tgttagtaaa taaacatgat ttgggagtag cggttaatac
    33181 tgcaacagat tttatatata aaactataaa ttacacatct caatttgata ctgatagaaa
    33241 tgatggagtt atgtttgaaa actttttatc agatttaact aatatataaa gtcaaaaaaa
    33301 ctatccattt tataaatacc tataaatcat aaaatttagg tatttacaaa atggatatta
    33361 tttactaaac tattggtatt tcaattgtta ccatagcacc accagttttc acagaatttt
    33421 caatatataa aatcccatta tgtttttgta caatattatt tacaatatag agtcccattc
    33481 cataatgaga tttagaattt ctactcaaat ctcctgtata aaactgttct gtagcttttg
    33541 acaaatctgc tttagaaaaa ccacttccac aatctgtaac tacaaatttt aagtatttat
    33601 tacatactga tacacttatg tatagtttac tctggcttgg tgaataatct atagcatttg
    33661 aaattatatt cataatagct ctgaataaca gttctctatc tatagttata tttttaggca
    33721 aattatcttg tttataaaac tccacatcta agtttttagt actagctaat gcttctattt
    33781 gcactaagac atcttttata aactcactca tactgattgc ttctttattt aatatatagc
    33841 cattctctgt ttttgatata tctattaaag tattaatata tttttctatt tgagtgacac
    33901 cctttgagat atattccatg tattctaata actcaggatt ttcatttatt tcaattaata
    33961 agtcagtatt tccatgtata attgttagtg gtgtttttat atcatgtgcc agtgcagata
    34021 tctgttcctt tctattttct tctagcatcc attgtttttc taaagaatct tttaacgcca
    34081 acttcatttt atccatagaa aaaaggacat tattaatttc ataaatttta ctagattcta
    34141 caacaaaatc taaatcctgt tgttctattt tttctgtagt attttttaaa agctccattt
    34201 caatattaaa tttcttgcca aatacttttg ataatataac aatttcaata aaaaatatta
    34261 tactaaaaat aatcataccc aatgtctctg gttttggtaa atatgctctt aaaattggag
    34321 aactatactc tgatactatt ttgtatttta ttataaaaac ttcatctttc ttaaaaaact
    34381 ttatataact ttctgaatat gcatgccttt cttcaatatc tctcattaaa ttccatactt
    34441 cttttgattc ttttttatta aaggtacctg aaattaaact cccatccaaa gtataaaccc
    34501 catactcata tatacttgga attatatctt cagttacttt ttcacttgaa ataatctgtt
    34561 ctttatattt attaaattgt ttttctgcat aattagctgg taaaattaca cctgatgata
    34621 ataatacaga aaatgataac attaaaagga gtacaattga aattgtcata ataaagaata
    34681 aagataaata ttttataaaa atagttctta acttttcaac ttttttgttg ttagattttt
    34741 tacctccaag catttgtacc ctgtcctcca atagtttaat taattcataa ttaataccta
    34801 gccagttttt tatttgatta ttttatatct ttttattgct aagttatatt ttaacataat
    34861 aatataaata aacagtcaat ttttactcta ttcttctgat tttttacctt cccatttaga
    34921 aaaccaaata cataaaatta caaatgctaa aattgttaca aaaccacaca ttaaacctgt
    34981 tgattgatat gcttttattg attcaacctt tatatattca accgttttag aacttgaaaa
    35041 agacgaccaa atagttataa atctaacacc ccatgcacaa ggaaaatact gccaaatacc
    35101 agaacccaat ccagttaata ataatgctga aagtaacatt tcaactatac ctactccaat
    35161 tgatgttcct tttccgaacc ttaaacttaa gaataaatgt aatatgtatt caaataaatt
    35221 acacccaatt aaaattagaa ttgaaatcat ataaaagctt gatgtaaagt aagccttaga
    35281 tataccaaat ttaaaaccta atacagacac taatgttgct acaaaaccaa gacatactaa
    35341 gattatatat ttacttataa gagtaagata ttttggatta gatgttgtta acatatgttt
    35401 ataattccct gcataatatt cttgttcaac aaacattgaa cacacaacac ttgacaataa
    35461 aggaaaagct attgctataa cttgtaaata tgctactatc tttagtatat tatcagctcc
    35521 tgaaacctta taataccata aaaacaatcc cacccccatt aaagcaatgc aaatgtgcat
    35581 tagtattata aaatttctct ttaattttat taaatcagaa caaaatattc ttattaactg
    35641 tctcatatta cttcacctct ctattattga accaaaccct tgtagctaaa gcaaataata
    35701 caaataaaac aatagatatt aatactcctg gcaaaataac agcattattt aaaaattcac
    35761 ttccctctgg tacaggaaga ccgtttggca gaatatgcaa tacaacagcc attaatcttg
    35821 aaggaatcga atatggaaac atataccaca tttctgtagt tgagaaaaca gcaactccaa
    35881 caatagtaaa gaacatattt attagaactg taacaaaaat accaaatttt gaagctaaaa
    35941 acatacaaag aggaatctgc cacataaata gtaaaataat taatatagtt gcaatgatat
    36001 tttgacctat tgaaatagtt tctttataga ttatcccaac aaaaactaca cctattgcag
    36061 aaataattga tgctataaaa caacaaataa tacagtaaat catttttcca gtccaaacat
    36121 ctttcatttc aattggcaat ggtaaaatac cacgatattt acttttttca tcttttaata
    36181 ctattaaaga acatattaat gttaatgctc ctggaagaaa tgtcgtatac caccagttga
    36241 aagcagcatt ttgccctcca cctagtccac acaaaagtat acaaaacact ggaactatcc
    36301 atatcaactt cattccaaaa gttcctttca tcttttgaaa ttccactcta ataaatcttt
    36361 ccatattact gccctctctt tctattttca gctacaactt tcataaaaag ttcttctaag
    36421 ttttgttctt ttgatacttc tccttgataa cccattactc cattagaaat tattcctatt
    36481 tcatctacaa cttgttcaac ttcagacaag atatggcttg ataaaataac tgtaattcct
    36541 tgttttggaa aagaacgaat caattctcta agttcttcaa taccaatagg gtcaagacca
    36601 tttgtaggct catctaagat taaaagtttt gggtgattta ataatgcaat agcaatacca
    36661 aggcgttgct tcatgcctag agagaattga cctgaacgct ttttaaaggt atctcttaaa
    36721 tctacagtct ctaaaacttc ttcaatgcga gagtcaggaa gaccaagtaa agtagtatgg
    36781 acttttaaat tttctcgtgc tgttaaattc tcatatatag caggagactc aataagagaa
    36841 ccaatatttg ataaatcctt tctagtccat ctatgtccct caaacattat ttttcctgaa
    36901 gttggattta aaatcccagt tatcattttt aataaagtag atttcccagc tccatttggt
    36961 cctaataatc catatataga attagattta attgctagtg aaatgttatc aactgccatc
    37021 tgctccttaa attttttaga aactcctttt acctgcaaaa tataattact cataattttc
    37081 atcctttctt ttgtcaaact tatttacaag aatcattcaa aatcataaat ataaggattt
    37141 tataagaatt aaaaaacaaa aaaagatgtg ttaaaaacac atcttttttt atcttaattc
    37201 tattaatttt ccattgtagt atatctctac ttcttcaatc caaatgctat tatcttcttc
    37261 ttggatgaca tttttaataa tattatttat agtatatatt atattttgtt cattcaaata
    37321 atcatctttc caagaaactc tacttttttt atctggttct atcaagttta ttttaacaag
    37381 attatctttt tccttaactt ctacttccat gtctaaacca ttaaaatact catttgataa
    37441 tgtatctaag attaatcgca gtttatcctt aagtgaactt tcttcatcaa tctgtaagta
    37501 ctttactatt ttctcatcct ttgaaattat ctctataggt attttagtag tttttttaga
    37561 attaatcatt ttactagttg tttcttcttt tgcatcttca tccttcttta caactgctga
    37621 ttctttagaa tcaactagct caactgcact gatgctataa tccatacatg ccgtactcaa
    37681 taaagtagta gcaattatta aaaaacttac tgctatttta ttcataagta tccctctcct
    37741 aattctcttt cgatacttat tataccatag tatagattat atttttatac ttttaaatca
    37801 atttataaat tattatagta aattatttac aattctaagc tatttgaatt tatttttata
    37861 gtatattctt tctctaaatt cgacagattt ttattcaaaa gcccttttat ttccctcgta
    37921 tttctatact tgttgctatg ttcttcatta tgttcataat catatgaatc attgtcatat
    37981 tcttcatcat tattaaattc ttcaattgct tcttttgact ttatatcaca attaaattta
    38041 tacttattta ctggtatatc aagtcttacc ttagaatttg gaacttctat gtccaggaac
    38101 ttagcaattg gcatatcaga gtacaagtaa aattcacttt cataatttga atttctacct
    38161 attttctctt ttatatttac attttcaggt atatactctt ctatattatt gataaataca
    38221 tcttcattct ctgaagatat atacaatgac tctgattcta tgtttacatt tttaacgcct
    38281 aaaattgatt ttgttgacaa gtgaagattg ttattagata ttaagtttag gttttctaat
    38341 ctatttactt tgttctctgt tatagctcta ctaaaacctc cataagaagt tttatatgtt
    38401 atagtatttg aagctacatt atcgtataca aataagtgtg agcttatact tgatgcattt
    38461 atgtctacat tatttggtac atatattata atttctctat aatcatgtga aaacatacta
    38521 tttatagatg tatttaataa ttggtcgaaa tttttaatct ttttaatttt attttcatat
    38581 tttctttcac cttttacaac taaagtctta tcttttaaag ttacattgta tttgtaaaac
    38641 tcattatttc ctcttgttgt tacacgcaca tcttcacttg aacttttctt tattgtaacg
    38701 tttatattgt caacagctat atctaacttg tcaatattta ctttttcttg ataaagttta
    38761 ttttcttttt taaattcagc ttccttttct aaaccaaagt ttattaggct tggcataact
    38821 aaaatccctg accatgttgt ccctattata cctattacca ataaaactgc agcaaatata
    38881 gcaagttttt tattcatgtt tatcttctcc tttcttattt tctatatctt caaggtcctt
    38941 atatatcgcc tcaaaattta tactatcatc attatattca tcagtttctt ttatgctttc
    39001 ttcttctcta ttgtagtaat cattattata tgaattttca ttaatgtctt tattcaaatc
    39061 ttcatcattt aaagtagctt tttttctttt ttccatcttt tcatttgcct ttatataaat
    39121 attttttctc tttatccaat gtatataatt atttagtaat tgtttaccta gttttatggc
    39181 aaataataaa atttgccatg caagtatctc aagccctaca aatgctatgg ttgcgaaaat
    39241 acaaaacatc ataacatctg gaaccattgc tataaattta atcacaaatg gaattaatat
    39301 aacaaaagca attaatgagc atactaatgc aatagtagca gatacaatag cacatacaaa
    39361 cgcaaatgct ggaacaatta gaaataaaga taacactact aataacccct ttataacttg
    39421 tctggataat cctcctacac cttcttctat acttggtgta agtttatcat ttatattttc
    39481 ttttgattta ttgtactttt ccttagcttc atctttcaag atagagtaat ccttttttac
    39541 atttgattta aatctactaa atttaccatt taatatgtct gtattcatta tttttccatc
    39601 tatattttct tttgatttac tatacttttc ttttgcttca tcttttaaga tagagtaacc
    39661 ttttttcata tctgatttaa atttactaaa cttatcattc aaaacatctg tatttattat
    39721 tttttcctct tcaatatttt tttctttagt ttgtaagata agttcttttg ctatagattt
    39781 tggtgagcca agtgatgcta ttatctccat atcactcttt ccctctataa gaccatctac
    39841 gaaatactct tcataatctc tcaatatatc attaacttcg ctctcagaaa aatctttttt
    39901 tagataatcc tttaaaattt caagaaactc agttttattc aagttcatta cctccctcat
    39961 cttcacttat taaaatctca acagccttta caaattctct ccactcttcc atcagattgg
    40021 ataaattctc ttttcctagt gaagttattc tatagtattt tctagcagga ccttcattag
    40081 attctagtat atatgtttcg aaatacaaat cttttgtaag tcttcttaga ataggatata
    40141 ttgttccttc atttacatct attactttag agatgttatg tactatctca tatccataca
    40201 tatctttctt agatatgaga gcaagtacac aaatttctaa aacacccttt ctaaattgtg
    40261 tgttcatata ctccatcctt tctttaaata cacagtattg tgttttacaa ggtactatgt
    40321 taaaacttct tatatcctca taataacaca gtactatgtt ctgcacaata gttattttta
    40381 aatattcttg ttgattaaat ccttctataa atgtataata ctctaatcag atatatttta
    40441 tgaaagtggt gataaattgg ctaaaatgaa agatgataaa acaatattaa tcctaactgc
    40501 tcaatttggt gctggacata taagtgctgc aaaagcagtc aaagaatgta ttattgaaaa
    40561 atatagtaac tataatgttg ttatacaaaa ctttataaat gcaagtattc ccatgatgaa
    40621 taagcctatg gtaaaacttt atgaaaacaa tacaaaatat actcctggat tatataatta
    40681 ctattattac tttaagaaat cgtttgattc aagacatgat ttatctcata aattatatac
    40741 acctaaactt tctgagtata ttgctgatat aaatccagat ttgattatct ctacatttcc
    40801 actagctgct gcttgtgtaa ataactttaa gataaaaaat cctgatataa atatacctac
    40861 acttacagtt ataacagatg ttgtagatag tatggaatgg gtttttgaaa atacagattt
    40921 gtattttgtt ccttctcctg aaataaaaaa tagatttttt cagaaaggaa taaatccaga
    40981 ctccataaag gttactggag ttcctgttga taagagattt caaattgaaa gtaaagaact
    41041 atgttgtgat aaatatagac tattacttct aggtggagga agaggtctat ttgatataga
    41101 tgaggatttt atgcattgga tagatgaatt tattgaagaa catagtaatt ctatagaaat
    41161 tacaatagtt actggtaaaa acaaaaaatt atatgacaat ctaactcata aaaaacctct
    41221 aaaaaatatt aaagtacttg gatttgtaaa cgacatgtat aatctaataa gagaatgtga
    41281 cctaatgcta actaagcctg gaggagcaac tatatttgaa gctattcaat cacaaactcc
    41341 agtactcgta aaaatgccaa aagttggaca agaaattgaa aacgctaaat ttataataga
    41401 taagggtctt ggcatgattt atagtgatga cctagacctt aaaaacatat tttataaatt
    41461 agtatcaaat gaatttgatt ctataataaa ctttatgaaa aaaaatttag aagaatttaa
    41521 aacagttatt catccagaga aaattgctga ttatatatca gaattaatag ataatcatta
    41581 cagttaaatt gtactattaa aaaatttcaa acataaaatt aaaggcaatc aagtatgctt
    41641 ttacacttga gaatttactc atagtgttta ataatacttg attgccttta tcatatcaaa
    41701 atcaaattgt tatattagta tatatttttc ttgtactgca tatacagttg ttgtatttct
    41761 tccatacttc tctttaattc tatttgtaaa tctgaagcag tctggtaatc cttattttct
    41821 atagcttctc tcactctagt gaatagtgat tctctagtta aagtatcctt cataatattg
    41881 aactttaaat ccgatattga gttccaaact acctcatcaa gcgcatccat attttctttt
    41941 gtgtgtatct tgacaatact tcttattttt tgaatcccag cctctattat tctagttttt
    42001 aatttctttt gccacttatc tatagctcca atcttaaatg attctattat actatcagta
    42061 aatacatcac cttgttttaa agattttaat tttgaagcat atatttctaa gtttttcata
    42121 ttttcataaa ctgtagctgg tggtattcca aatcttgcat ttctttcttc caaactataa
    42181 tgttcaaata catcattttc atctctatat gctctatctt tttctaaata gaaactttct
    42241 tcacctacat ttttagataa ctctttttca agttcttttg tactaaggcc actcttagct
    42301 gctgctttta taccatccaa cattgtttga taacatcctg ctattactaa atacgtgtta
    42361 gatagagggc ttggtgatct taactcaaat cttacagtct ttggattttt catatctctt
    42421 attaatccaa caagcactga cctatttctt gatggaatct cataactatg ccctaaagat
    42481 gtaactatgc atacaggagc ttcaaatcca ggaactaatc tattaaagcc atcattagaa
    42541 gctgttacaa ttgggtttaa tacttcataa ttgtaaagaa gtcccataag agccccatat
    42601 cctagttcac ttagataatc ttcttttaaa tcttttggag caaatagatt ttttatactt
    42661 ccatccttta acttcgcact tactcctaca tgtgtatgtc caccacttcc tgctactcca
    42721 tgaattggtt ttgctttaaa tgtaacttct agtccatgtg atgtaaatac atcctctata
    42781 acatctctta ctaataattc attgtctgct gcttgtaatg gtgtagaaaa cttccaagaa
    42841 acctctaatt gctccatagc atggtttgtt cttccatcta tactaataga actttgtatt
    42901 cctccgacct ctttgtgagc catttcaggg tttacaccta gcttttgaag tataattaaa
    42961 cttttctcta aacaagttct tataatacca tgagttcttt tccagtattg ctcttttagg
    43021 ctttgtgata catataattt ctctaagtca gctttatcct caggagtatt aacccagaac
    43081 tcaagttctg tagctgctgt taacattatt tcctctatat catctactga atctattcct
    43141 atgttattta ttacatgtgg atattctcta aatatttcat acattgactt tttaaagtac
    43201 ttatctgctt tttgaagtac tcctctagaa catacttttt tattatcatg tattaaaaat
    43261 gcaggtatct ttaatgttcc aacaggtaga cctacttctt cgtctatata ttccatattg
    43321 taatctacat accaatgaca acttttatct ggcattaaat ctactttcgc attatttaat
    43381 gtagctatat tataaagctc aacacttgaa ccatcagtct gtatagctga ttccaaaaat
    43441 ttatctatat cctccaaaaa taattctaca ggaatctttt catctgttgc atttcctcct
    43501 aaatcaaccc ccattaagga aacaaatctt atatttttat tgctttttag gatttctctt
    43561 aattcttcat tagtgtgttt atctttctct attacgtata atagactatc catttttcca
    43621 ctctcccata atttttatgt atattttatt ttgttaaata caagataaat ttcattttcc
    43681 ttacatttat gaatttttct tgcaaattat tcgtgtttta ttcgtgtttt tatttatatt
    43741 attatatatc aagtataaaa agt
//
LOCUS       DAFKMX010000009        41103 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFKMX010000009 DAFKMX010000000
VERSION     DAFKMX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.009"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            108..1499
                     /locus_tag="LZB08_000397"
     CDS             108..1499
                     /locus_tag="LZB08_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBM7313918.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            1676..2263
                     /locus_tag="LZB08_000398"
     CDS             1676..2263
                     /locus_tag="LZB08_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7313919.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARFFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            2296..3087
                     /locus_tag="LZB08_000399"
     CDS             2296..3087
                     /locus_tag="LZB08_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7313920.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(3268..5685)
                     /locus_tag="LZB08_000400"
     CDS             complement(3268..5685)
                     /locus_tag="LZB08_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7313921.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VFFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMEIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKNVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(5698..6363)
                     /locus_tag="LZB08_000401"
     CDS             complement(5698..6363)
                     /locus_tag="LZB08_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7313922.1"
                     /translation="MSILQVINLKKYYEQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(6445..7476)
                     /locus_tag="LZB08_000402"
     CDS             complement(6445..7476)
                     /locus_tag="LZB08_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7313923.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALTIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(7466..8152)
                     /locus_tag="LZB08_000403"
     CDS             complement(7466..8152)
                     /locus_tag="LZB08_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7313924.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTVKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(8310..9374)
                     /locus_tag="LZB08_000404"
     CDS             complement(8310..9374)
                     /locus_tag="LZB08_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBM7313925.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            9636..10307
                     /locus_tag="LZB08_000405"
     CDS             9636..10307
                     /locus_tag="LZB08_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7313926.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            10309..11217
                     /locus_tag="LZB08_000406"
     CDS             10309..11217
                     /locus_tag="LZB08_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7313927.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            11294..11980
                     /locus_tag="LZB08_000407"
     CDS             11294..11980
                     /locus_tag="LZB08_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7313928.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            11977..14532
                     /locus_tag="LZB08_000408"
     CDS             11977..14532
                     /locus_tag="LZB08_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7313929.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(14726..15925)
                     /locus_tag="LZB08_000409"
     CDS             complement(14726..15925)
                     /locus_tag="LZB08_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBM7313930.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            16044..16931
                     /locus_tag="LZB08_000410"
     CDS             16044..16931
                     /locus_tag="LZB08_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7313931.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            17121..17678
                     /locus_tag="LZB08_000411"
     CDS             17121..17678
                     /locus_tag="LZB08_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7313932.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(17917..18777)
                     /locus_tag="LZB08_000412"
     CDS             complement(17917..18777)
                     /locus_tag="LZB08_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7313933.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTVLDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            19276..20679
                     /locus_tag="LZB08_000413"
     CDS             19276..20679
                     /locus_tag="LZB08_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7313934.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            20898..23375
                     /locus_tag="LZB08_000414"
     CDS             20898..23375
                     /locus_tag="LZB08_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7313935.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRILIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILFPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            23390..24076
                     /locus_tag="LZB08_000415"
     CDS             23390..24076
                     /locus_tag="LZB08_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7313936.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNEADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
     gene            24350..25183
                     /locus_tag="LZB08_000416"
     CDS             24350..25183
                     /locus_tag="LZB08_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7313937.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            25221..25682
                     /locus_tag="LZB08_000417"
     CDS             25221..25682
                     /locus_tag="LZB08_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBM7313938.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            25773..25961
                     /locus_tag="LZB08_000418"
     CDS             25773..25961
                     /locus_tag="LZB08_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7313939.1"
                     /translation="MAHMDFAVNDLEEAVQYAIHCGATIAEEQFTDDWRVMIDPAGHP
                     FCLCQMKSIMESSHFSLL"
     gene            26414..26734
                     /locus_tag="LZB08_000419"
     CDS             26414..26734
                     /locus_tag="LZB08_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBM7313940.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            26872..27516
                     /gene="trmB"
                     /locus_tag="LZB08_000420"
     CDS             26872..27516
                     /gene="trmB"
                     /locus_tag="LZB08_000420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBM7313941.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            27645..28391
                     /locus_tag="LZB08_000421"
     CDS             27645..28391
                     /locus_tag="LZB08_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313942.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(28520..28798)
                     /locus_tag="LZB08_000422"
     CDS             complement(28520..28798)
                     /locus_tag="LZB08_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313943.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            29073..29519
                     /locus_tag="LZB08_000423"
     CDS             29073..29519
                     /locus_tag="LZB08_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313944.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            29789..30241
                     /gene="accB"
                     /locus_tag="LZB08_000424"
     CDS             29789..30241
                     /gene="accB"
                     /locus_tag="LZB08_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBM7313945.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            30257..31618
                     /gene="accC"
                     /locus_tag="LZB08_000425"
     CDS             30257..31618
                     /gene="accC"
                     /locus_tag="LZB08_000425"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBM7313946.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            31633..32487
                     /locus_tag="LZB08_000426"
     CDS             31633..32487
                     /locus_tag="LZB08_000426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBM7313947.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            32508..33452
                     /locus_tag="LZB08_000427"
     CDS             32508..33452
                     /locus_tag="LZB08_000427"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBM7313948.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            33641..33901
                     /locus_tag="LZB08_000428"
     CDS             33641..33901
                     /locus_tag="LZB08_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBM7313949.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            34054..34425
                     /locus_tag="LZB08_000429"
     CDS             34054..34425
                     /locus_tag="LZB08_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBM7313950.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            34455..35300
                     /locus_tag="LZB08_000430"
     CDS             34455..35300
                     /locus_tag="LZB08_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313951.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            35621..36748
                     /locus_tag="LZB08_000431"
     CDS             35621..36748
                     /locus_tag="LZB08_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7313952.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            36852..37487
                     /gene="lexA"
                     /locus_tag="LZB08_000432"
     CDS             36852..37487
                     /gene="lexA"
                     /locus_tag="LZB08_000432"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBM7313953.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLHPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(37634..38791)
                     /locus_tag="LZB08_000433"
     CDS             complement(37634..38791)
                     /locus_tag="LZB08_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7313954.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILLGTIAESKKNDPCDICYGK"
     gene            complement(39120..39752)
                     /locus_tag="LZB08_000434"
     CDS             complement(39120..39752)
                     /locus_tag="LZB08_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBM7313955.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            complement(39745..41102)
                     /locus_tag="LZB08_000435"
                     /pseudo
     CDS             complement(39745..41102)
                     /locus_tag="LZB08_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        15416 a         5007 c         6042 g        14638 t
ORIGIN      
        1 tgtttatata atttgcatgt tatatactaa ttttaatgac attgataatt tataaacaat
       61 tataaagttg taaacttata agagttattg aaaggtggag tgaagttatg gaaagactcg
      121 ctagaaggga acaacctcat ataaaatttg gaatgtttaa gataagaatt ccatttatac
      181 attacagatt tgagaaacca gaagctattc aaggtataat atcaagtatg acaagtttag
      241 gcacaattgg ccttagtaca caaattttgg gattacccta tgaagtggca tggagtatgg
      301 ctattattaa ctcaatatta tactgtttgc atgtttttat gggagaccca gttgtacctg
      361 gctggattac agcttctata actcttacaa ctgcatattt gttaaagttc agcatgggca
      421 ttgaaagaat acaagcatta acagctttac aaatagattt aggaataata tttattttaa
      481 tgggtataac tggtgtagct ggaaagttag ttagtaagat accaaattct attaaaggtg
      541 gaatactaat gggtgtcggt atatcgacta taatatcaga gtttaatcct aaagggagat
      601 ttgattctta tcctatatct ataacagtag gaattttggt tgcttgtttt gtaatgtttt
      661 ctgaaagatt tgatactcta aaagttaaga ataaattttt atttcattta ggagaatatg
      721 gtgtagtatc agctatttta gtgagtttaa tagtaggtat atttagtaaa gaaatagaaa
      781 ttcctagatt tagttttgac aatttagttt acatagtaga ttttaaaaat ctgataaata
      841 cagtatctcc ttttggaata ggttttccaa gtgtaatgtt atttatacaa gggataccaa
      901 tggcttttat gatatacata attgcctttg gtgattttat aacaggtgaa aacttagtac
      961 tttctgaaag tgaaaataga aaagatgaat atatagattt caattcaaat aggtctaatg
     1021 ttataagtgg gataagaaat atttttatgg caatcttttc tccatatata ccaatgtgcg
     1081 gtccattagc tgcgacactt acaggaagtg ttgctcaaag gtacaaggtt ggaaaggaag
     1141 ccatgcagtc tgtttttagt ggcatgggta cattagtatg ggtttcagca atttttatct
     1201 gtttttatcc aattgcgcag attgctacac ctcttatacc attagcattg tcagtaacac
     1261 ttttagttca aggatattta tgtacaaagc tatcaatgga gctatgtaat acaggtgttg
     1321 agaagggatt gattggactt atgggaggtg taattgctgc taaaggcgga acatggggac
     1381 ttgctgtagg ttttatactg tatttcattt taattgattc taaaaaaaga aaagaaaaag
     1441 atattaattc aatagaagat tgtgtatatg aaatattaga caaaaagaag agtatttaaa
     1501 ggaaaatctg agttgctgtt atctattaat agattaattg caactcatgt tttatttata
     1561 agttttagga attgcaaaaa aatgcaaagc tttttcttga cataatttct tgatatgtta
     1621 ctatagttat ataacacatg tgttttataa aaatttaatg gagaggtgaa catatatgcc
     1681 acctaaagta aagataacaa aagaaatgat tttgaataca gtcttggata ttacaagaga
     1741 agtgggattt gaagctgtaa atgcgaggag tattgcaagt aaattacagt gttccactcg
     1801 accaattttt acttgttatg aaaacatgaa tgaattaaaa aatgaatttc ttgcttttgc
     1861 atatgaatat tataatcagt atgttgttaa ttatcgtaat tctaaaagtg taagccctta
     1921 tttactgctt ccgctttcat acattgaatt tgctcaagaa gaaacgtatt tatttaagtt
     1981 gttatttata aatgatttag atttgatgat gaaagaggcg aatgattttt ataaagaaat
     2041 tgacaatgaa aaaaaggcaa gatttttttc ggaaagcatt ggaatagatt tggaatgtgc
     2101 aaaagtaata tttttagatt tatttcttta tactcatggc atagctgtct taacagcaac
     2161 aaaaaaatta acattagata gatatagtgc tgaaaaaatg ttgaaaaata tattaacagc
     2221 ttttataaga caagaaaaat cagattggaa tttgtctatt tgatgtttat taactaatat
     2281 agaagaggtg aaattatgga aacaaaacaa tacttaattg acttttataa tacttatgat
     2341 gaagatagtc gacttgcatt aaaacatgga atggtggaat ttctcactac tatgcattac
     2401 attgataagt acataaaatc aggtgattgt gtgcttgaaa ttggcgctgc aactggtcgt
     2461 tattctcaca cactggcacg tcagggatat gatgttgacg cagtggaatt agtcgaacat
     2521 aatatagagg ttttccataa aaatactcaa tcaaatgaaa atataagcat tactcagggt
     2581 aatgctatgg atttatctgt tttcccagat aataaatatg atattacgtt actgttagga
     2641 ccactatatc atctttataa caaagaagat aaacaacaag ctttacatga ggcaattcgt
     2701 gtaacaaaac ctggtggtgt ggtttttgct gcatatgtta tatctgatgg ctgtctcatt
     2761 gacgaggggt tccatagagg aaatattaat gtatctgaat atattgaaaa aggtttgatt
     2821 gatccacaga catttgctgc taagtctgaa ccaaaggatt tgtttgagct tgtacgtaaa
     2881 gaaaacattg atgatttgat gtctgcattt aatgtaacta gactgcatta tgtcgcatca
     2941 gatggtttgg ctttatatat gcgtgaagca gtagactcta tggatgatga tgcatttgca
     3001 ttatatttaa aatatcacct tgctacttgt gaacgtgaag atttagtagg ggttacaagt
     3061 catgcaattg acatatttag aaaataagga atatacttgt ttatgtctct ggtgatgttg
     3121 ctcggagata taaggaagtg tatctgtata aaataaaaac aagtataggg atagaaaatt
     3181 atgtaaagtc aaaattttct atccttttag cttttaaaaa attatgttaa aacttgtata
     3241 aaaaatggct attttttata tacattctta ctgagaacgt aagcgttctg taagtgattt
     3301 tttttctata tttcttacaa gtaattcagt cactaaaact tgtataccaa ttaatacgaa
     3361 tgaaaaaatt aaaatagtaa atatagaaaa tttaaaatta ataaagtcta atctaattac
     3421 attttttata aaagtgcaaa gtagatttga accaaaataa cctagtatac ttgaagtaca
     3481 tatagctttg atacttatgt ttaaaccttc tctataaagc atatttcgca gttgctttct
     3541 tgtcattcca acggcttgaa gtaaggcaaa ctcttttttt ctaattaaaa cgctgtttat
     3601 tagggtattg gtcatattta gtataccaaa gcaagaaatt aaaccaacaa aaatgtatga
     3661 agctataatt atatattgaa atgctctagt aatcattcta taatcatctt catatgaata
     3721 aagcattaaa tttgaatttt tctgaattat acttaacaac ttttcttcaa cttctttaga
     3781 atgcttttta tctattaaaa tagaaaaatc taaatttgta ttatatggtg ttagagattc
     3841 taaagttttg tctgaagcaa aaaacatacc tcctgtattg gaattgtcaa tgattcctat
     3901 aatttccatt ttctttttaa taatatcatt tccactaaag atattaaatg taactgtatc
     3961 tccaactttt aaatttaatc cataatattt tgttctatat ttatttataa ttaaactatc
     4021 tgaatctaaa tgtgtatagt ctatttttcc actgacaata tattcttcaa gtgattttga
     4081 aagttcaggc gtaatacctt taattagagt aataaattgt ttttcgtctc tatctttgat
     4141 gtaaggttct ataatattac ttttaattga tttggattca attaattttt ttactccatg
     4201 aatagattta atttcattct taagagaatc tgttagtgga ttattttgtt gtatttctgt
     4261 aatatgctca tataagttat cactcttaat gctaattata taatcttcgt tatagacaaa
     4321 ttgttgagat agttgttttt tgaaatctat tgaattagca agattagcga ttgaaataaa
     4381 taaaactcca ctaatgctta gcgatattaa tgcaatcaaa tttttctttc tatttgactc
     4441 gatattcttt aatgctagat tttttatatt tatataacta aaattttttg ctttttttct
     4501 aaatttagtt tctgtatatt gcatggcttc aatagcagat attttagaaa taattctagc
     4561 tggtttaaat aaagaaattc taacagttaa aaacattacc agtgataaaa ttaaaattat
     4621 aaataaatca gctatcattt gccatgctaa tggttgaatt atgtaagcaa ttatacaaga
     4681 agctataacc cctaatggaa taaaaataat tgacaaaata tttccttgta gagaaataat
     4741 cttttttaac tgaaaagagg tcatacctaa cgatttcaac ttagcataca tctgaactga
     4801 attaacaact gaaatataaa aaatactata tataacaaaa acgctagaaa aaaaaacaac
     4861 tattccaact actaaaactg taataatctg ctcaccatca attaaatttg attcaatata
     4921 gttatcattt aagtatatat tatatttctt aatacctaaa gataaaccaa ttttctttaa
     4981 ctgtgtctta attgaagctg aatctttctc tgtattttct tctgatagtt taactaatac
     5041 atctacagta tcaggtcttg actcgtcaaa agtattactt ttattttgta aaggtgtaga
     5101 agctatattc ttaaaataat catttgaaac gactgtatag tattgcttgg cattatcttg
     5161 ataataattt tctagaaatc caacaataat aaaatcacat tgaacttctt tattcgtaat
     5221 atcatcataa taatttaatt taatgctatc tccaatatct ttatctaaac caaattcctg
     5281 taaatatttt tctgaaatag caatttcatt tttatttgta ggtagtttgc ctttcaatgg
     5341 ttgaaaattc ataagtttaa ttgctgtatc atctgcataa acaatcgaca taatacaatc
     5401 tgtcttttta ttacttccaa ttaatttgta agttcctacg ccactaaagt ttttattttt
     5461 atatagtata ttttttgctt tttcatctac agatttatat atagcatgat atggagatgc
     5521 attaactaaa tctacagaag cattatatga aactaaggtc actgttaata ataaacatgt
     5581 agaaatcatt ataatgatac ctgaaagata tgttttaagt ttattaattt ttaaatcatt
     5641 taaagctatt tttcttataa atgtgcgatt attatttcta aacatatact acacctccta
     5701 gttaacaatt tttccatctt caatacgaat aattctatca gttagttgtg caatttcatt
     5761 attgtgtgta atcattacaa ttgtttggtt gaatttttta cttgtcactt taagtaaact
     5821 cagtacatct gaactagttc tactatcaag gttacctgtt ggttcatcag ctagtataat
     5881 agctggtttt gttacaagtg ctctagctat agcaacacgt tgctgttgac caccagaaag
     5941 attacttggc atatcatata atttttcttc aagagaaagc atatttataa tttcatttat
     6001 aaaatcctta tctatggtct ctccatccaa tcctattggt aagataatat tttcatatac
     6061 attaagtaaa ggaacaagat tataattttg aaatataaaa cctatatttc tgcgtctaaa
     6121 gattgtaagc tgttcatcag tcatagtaga taattcctta ccctttataa taacctttcc
     6181 agatgtagga ttatctaatc ctcccatcat atttaataat gtggatttgc cactaccaga
     6241 tgtacctaca atagaaataa attcgccttt ttcaatagat agagttacac catcaagagc
     6301 ttttgtaatg tttgaacttt gttcataata tttctttaaa ttaattactt gtaaaatact
     6361 cataataata cctccttaac atcttgtaaa tacagtataa aaccaaaatg ttgcagaatc
     6421 attacaaatg aaatatttaa aaatttattt attaggtaag aaaatagaaa aagtcgaccc
     6481 ttttccaata tctgatgtaa ctttaatata gccaccttga agagatataa tttttcttga
     6541 aagatagaga cctattccaa taccttctat gtcatggaca tcttcttcac gataaaatct
     6601 tttaaagatt tctgcttggt gactttctga tattcctttt ccattatcaa taatatctat
     6661 ttttgtatac ataacccaac attctgtagc tacacgtatc ttaccattag aatttgtgta
     6721 ttttacagca ttatctaata tattaaaaat agcttcagaa gtccatttct tgtcatgaat
     6781 tatatataaa tttgggtcac aatcaactgt aacttcaata ttcttttttt cagcatcaaa
     6841 caaaattcca ctaagtgctg tagcaattgt ttcataaatt gaatttttct ttttagatag
     6901 tgtgattata cctgtctcta gtctggaggt ttttaccata gattgcatta aaaaatctaa
     6961 tttatctaat tggttttcca tatccattaa aaaatccttt tgaatttctg ttttaaatgt
     7021 ttgatttagt agagtagtat taatcatttt taaatttgca ataggagttt taatttgatg
     7081 agatatatca gaaactagtt cttgtaaatc agctttctct atagcaatac tattgcgatt
     7141 ttcttgaata acttcataca ttctaattaa tctgtgattg atacgtgata aaatagtttc
     7201 ttcttctaat tcaatttcaa tattatcttc actattcatc atactatcta aagtgtgaca
     7261 tattttagaa caaaatataa ctattttttt atgaagtaaa aatattgtta atgctataaa
     7321 tccacaaagt actattgtat atattaataa tagagcaatt agttttaaat catcagttat
     7381 tagataaatt gagatactaa ataaaatagc tgtaaaaatt ataccaacag caattattaa
     7441 ataaatagaa ctaaaagaaa attttttaaa attcatttct ttcacctaac cacatatatc
     7501 ccataccata taccgttttg atatacttaa gattgtcttt ttcaattttg cttctaatcc
     7561 tacttataac agatgttaaa gtatgttcat ctacaaagtt agcatctata tcccagagtt
     7621 tttctaaaag tactttcctt gttaaaatac ttttaggatt ttcagtaagc agttttaaag
     7681 tacgatattc taatggtgta aatataattt gatttccatt tatattggca gataactcag
     7741 aaaagttaat ttcaagatat ccatccctat agtaatcttg ttttgttttt actgtaagat
     7801 gatttaaaaa agctttaact ttctgtttaa atatattgat attaaatggt tttgttatat
     7861 aatccaaagc tccaagttca tagcctttaa tcatatcaca ttccatatca ttagcagtta
     7921 aaaaaattat tggtatattg taatttccct ttatttcatt acataattca aacccacttt
     7981 tatctggtaa attaatatcc aaaattatta aatcaaattc acattcaaaa atagacttca
     8041 gtgcagattt agctgtaaat tttgaagtaa ttatatatcc atcttcaatt aggttatatg
     8101 atagagtttt atttaatata gaatcatctt ctactactaa tattttttcc atacagttac
     8161 tccttttagt ttaatatttt ataatgtcaa ttatagacta aaaacattgc tgaattgtta
     8221 taaacgagaa aaagtattta aaaatttata tatttaataa agaacctgtc ctatgataaa
     8281 ttctagtaca gattctttat taaataaaat tatttcaacc aagatggatt tccaggtata
     8341 aatattcttt taccaaattt tgaagtatac tcatctaaag ctttttgaat aagctcatta
     8401 ttacccttaa atgaagtagt attctttata gtttcgagat tttggctaaa tgggcaagca
     8461 gatatacaca taccacaatc tgtacctaaa tatctccatt taatatagca tgtctcatgt
     8521 tcaattaccc aattatattt tccatcatca ccaattttaa tatcattaga aagcgaatga
     8581 gaagggcagt taaaagagca ttttttacag accttacaaa aatcttctag tccaaaatca
     8641 acatattcat cttctaaaag aggaatatct gtagtaacaa cacctaatct aagtctagag
     8701 ccataatctt tattcgtaag tatagcattt cttcctatat ctcctaaacc agcatcacga
     8761 gctataaaaa caggcattac taaataatta gcatccatat gatttctagc atcatatcct
     8821 aaatttctta tatagtaact taaaatcatg ccgacgatag atgcatccac atagcacttt
     8881 gatgtttcta taacttcaca tatcattgga gctctattta tcatttcttt atccatctca
     8941 catccaaata caattgcata cttatgagtt agattaattt cttcaccgta attttcttca
     9001 tgtctacccc tatttgtata aatatgatag tcttttaatt tagttatacc aactacagaa
     9061 gctccatact gctttgcaag acccttaatt ttttttgtca ttgactttgc atctacatct
     9121 actttagtat cagaaacttt tccttcgcat aaagatttga tatcagataa aaaatcgaat
     9181 gcactacttg ccattggaga gtttaactca ttgtaagtca ttgttccttc actacataaa
     9241 tttggtctat ttcttatact atcatcaatt tcttttttgt caggattttt cttataatag
     9301 tcattataag cagtactatc ttttttgtaa tttgctcttg caaacatagt atttctctca
     9361 tcaacccttt tcaattctat tccccctagt aaaaatatat ttaaaaaata taactttatt
     9421 gttgtataat ttaattatat aagaataaag ttatattttc aatttaagcg taattaaatt
     9481 gggaaatata taactatatg cttgattatg atgtttttat tttgaaaaca aatttaaata
     9541 caatcttttt agtgaaataa ataaaattaa aatattttta gtatgataat gcaagaaaat
     9601 aattatttat ctaaaaaatc taaaggagag taaaattgaa cttattaatt attgaagatg
     9661 atataaactt aaatgaaggt cttttttatg catttgagaa tgatggattt aatgtattta
     9721 aggcttatac aaaacaagaa ggattaaata tatttaatag taaaaatata gattttataa
     9781 tcttagactg caatcttcca gatggagatg gatttgatgt atgtcaaata ataagggaaa
     9841 agtcagatat accaattata atgcttactg caagagactc agaaattgat gaagtaaaag
     9901 gattagaaat agggcttgat gattatatta caaaaccatt ttctttatct gtattaaagg
     9961 caagagtcaa agtagcaata agaaaaaaat cgaataataa agtaatatat tccaatggaa
    10021 taaaattgga ccaaaagtta ctaaaagtat ataaaaataa agaatgtttg gaacttagct
    10081 ctgtagaata taagcttcta agttatctaa ttgaaaataa aggtcaaata ttattaaagg
    10141 aacaaatttt acaccatatt tgggacagcg aagaaaatta tgttgatgat aatattgtat
    10201 ctgtaaatat aagaagactt agagtaaaag tagaggatga cccatctaat ccaaaatata
    10261 taaaaacagc ttatggaatg ggttatttat ggaatgaggt agaataatat gtttatatta
    10321 gcctttataa tatgtacagt tgttatattt atagtatcta ctaaaataaa tcaaaaaaga
    10381 tatgatgaat taaatttgat gttaaatcaa atattggaag gtaaagaagt tacttatcca
    10441 gatacaaagg atacaagagc gtcaaaaatt tctcaccaag taaaaaaaat taaggatatg
    10501 atagaaatag aggtagaaca gtcaaagtca gaaaaagaag cgattaaagg tttaatatca
    10561 aatatgtcac atcaattaaa aactccactt tcaaatataa ccatttattg tgaacttctg
    10621 gagaatataa atatttccac attacagaag aaggagtttt tacaaaagat gaaaaatgaa
    10681 acttttaaga ttgactggct gttacaatcc ttatttaaaa tgactaaatt agaggatgga
    10741 gttatagaat ttgaagtaga agaactacta attaaagata ctcttattca aagtatcagt
    10801 actatcttta ataaagcaga agctaaaaat ataaggataa atctcgaacc attttcagac
    10861 ataaaattag ttcataataa aaagtggacc atagaagcaa ttgtaaatgt tttagaaaat
    10921 gcaataaaat actcaccatc agatagcacg attactattt ctgtaataaa aatggaacta
    10981 tatacgaaga ttactattaa agatgaggga ataggtattg atagtagaga actaaatgat
    11041 atttttaaaa gattttacag aagtaaaaat gtggaaaacc aaaatggcac agggattggt
    11101 ctatatctta caagattaat tttagaaaaa gaaaatggaa acattatagt agaatctaaa
    11161 ctaggaagtg gatgttgttt tagtatcttc ttacagaatt gtaagagttt aaactaattt
    11221 tgtaggatga gtgtaattat cgtcacttac aatattttat agaacatata tctgtattga
    11281 aaggagaaat taaatgagta tattagtgac gaataattta gtaaaacact atggaaaagg
    11341 tgagactaaa gtaaaagcct taaatggagt aagtatagaa attgaacgag atacatttac
    11401 tgctatagtt ggaacttctg gttctggaaa aagcacattg ttaaatatta taggtggatt
    11461 agataatcca acttcaggtg atgtaataat taaaggaaag aacatatcta aaataggaaa
    11521 aaaagatttg acagtattta gaagaagaaa tataggattt atatttcaaa actacagttt
    11581 aatgcccgta ttaaacactt atgataatat agctcttcct gttacattag ataaaggaaa
    11641 ccatatagac catgaatata ttgaaatgct gatgaagaca ttgggaattt gggataagcg
    11701 tttaaaattt ccaagcgaac tatctggtgg tcaacaacag agggtagcaa ttgctagagc
    11761 tttggcaaat aaacctgcgc ttatattagc agatgaacca acaggaaatc tcgatagtaa
    11821 aactacaatg gaagtggtat gtttattaaa agaaagtagt gcaaaattcc atcaaacgat
    11881 acttatggta acacacaatg aaaatatagc acaagtatgc gactcaatta tccatattga
    11941 agatggagtt gtggtgaata ctggtggtga agtactatga tgtctctagt aaaattttca
    12001 attcaatata taaagcatta taaaaaacaa tctatatcta tagttttaag catagtacta
    12061 tcagtagcat tattgacggg aattggttct ttagttcata gtgcagacaa aagtagaatt
    12121 gaaaagataa gggaagatag tggagattat cattatttct ataaagtaga taaggaacaa
    12181 cttaataaaa ttaaagaaaa taaaaaatca aaggaataca ctgtagatag acttggcatt
    12241 acaaaaccta aaaatcagat agatgaacct tttattatag aatttttaaa tgctgattct
    12301 tcatatttag acatgacagg tagaaaatta ttaaaaggaa ctcttcctag taaagatgga
    12361 gagatagtac ttgacacata ttctctaaac aatttaaatg taaacaaaga aattggaaca
    12421 gaaatcaatt tagatggtaa aatctataag ttatgtggta ttttatcaag tctttcaaac
    12481 ccagaaactg ctatggaggg atttgtaaac gattattttc agcctaaaga aaaggattct
    12541 tatatagttt atgtaaaata tgatgaaaat aaaaatatta aaaagcaaaa ttataaattt
    12601 atgaaaaaat tcaatatatc aaaagaaaaa acttatataa actggcctct ttcagttgaa
    12661 tttggagtaa aaccaccaaa aaatatagaa aaatataata tatttgagtt cttaaaaaat
    12721 ttagaactaa atacaaatgc aattatattg cttgttggag tgtttggtgc agcagcagta
    12781 tatagtattt tccatgtttc aatacttcag agaatatcac aatatggggt tttagaagta
    12841 ttaggcgcta ataataaaca acttttattt ctactatttt tagagttatt tttacttttt
    12901 attgttggat ttcctatagg ttgcttctta ggtattggag tagcgtctac aatatatgaa
    12961 caatttccac atatattttt gagtagtgat attgttccag gagcattttt tataagtgaa
    13021 aaatctattt atttaggctt tttatttatc ttattgttgt taattttaat tgctgtacgt
    13081 gttgtacatc agttgaataa atactctagt atagaatcta tgaaaaactt taagattact
    13141 aataagaaaa agagaatatt agaaagtgta aaacactcta acatgacaaa agtattatct
    13201 cataaataca tgactcaaaa aaagggaatg tttattggaa ttttgttttc attatcactt
    13261 ggtggaatta tatttctatg ttctagttac tctatacagc ttactaaaaa taataatgaa
    13321 cttacaatga aagctgatga tggtttaaat tctgactacc aaataactat gcaaacaaca
    13381 gattttgatg taggtatacc aaaagataaa atttcaagat taaacaatgt tgagggaata
    13441 tcaaaagtct atccagtaag atatttattt ggagctatgc aaattaaaga agaacaacta
    13501 ttttggaaaa atttctttaa acctcttgaa aaagattaca ggataaagac tttttttgat
    13561 ggtatttgta caaagcaaaa tgaaggtggc tatcttttaa aaacaaatat ttatggttat
    13621 aacaatggta tgttagaagg attaaattct tatattatag atggggagat taatacccat
    13681 gatatggtta aaaataataa ggttatagtt agactaccta tggatggaac tggtatgtat
    13741 gatgcagttg atataaatcc aggagataca ataaaagtta aagtgccaaa gacaatgaaa
    13801 ccaacagatg aaattgttaa atttaaagaa gaaaataaga atgattatac aacaaaagaa
    13861 tttgtagttg ctgctactgt aaaacgtgtg atggcaaata acatatattt cattggtgat
    13921 tatggtatgg atatagttat gacgaatggt caaatgaaaa ataactttga tatcgaaaat
    13981 tataattcag taagtataaa aaagacagaa gatagtaaca gtgaggaatt gtctgagaac
    14041 ataaaaagtg ttgtaagtga tgtaaaaaga tgtattgtaa cagattatac cattgctatt
    14101 gaaaaaaata atacctactt aaatcaaaaa ctcttattta tatatggtat tgtatttgta
    14161 cttcttgcaa taagtttgtt ccatataatc aatacagtaa gctacttaat tttttcaaga
    14221 agacatgagt ttggaatttt aagagctatg ggtattacgg ataataagtt tttacttatg
    14281 atgataagag aaggtttttt atatggattc tatgcaagta ttataatggt aattggaagt
    14341 gttattggac aatttatgat ttattttatg gtaaaacgtg tatatttata tataaatcca
    14401 attcttaaaa taaatacacc tctatacata ggtatgatta ttttaaatat cactattagt
    14461 atagttgctg taattattcc agtaagacaa attttgaaga gtgatattat ttcagaaata
    14521 aataagaatt agagttatat aaatttttaa gattatataa ctttctgaca atcttgttta
    14581 aattatttaa atatatctta tctgtgaaca ttaaataaac ctcttaatag atataactct
    14641 tgaaaagtca tatctattaa gaggttttaa taaattttca tgttaatata tataatccaa
    14701 aaaatttaaa tcttattaaa acatactatt ttgtatagtt atcgaaatat ccttgtatgt
    14761 aaataatagg agtaccctta tctccacttc cagatgttaa atctgctaaa gaacctataa
    14821 ggtctgttaa tcttcttgga gtagtacctt gagaaaccat gtttcctgtt aaatcatcag
    14881 atttattatc tttttcaact atgtattttg aaattgcttc ttttaactca tcaccagata
    14941 agtcagcaaa atcattatct gctaagtatt ttaattttac ttcgtttgga gttccctcta
    15001 gcccttttgt ataagcagga gatactactg ggtcagctaa ttcccatatt ttacctacag
    15061 ggtctttaaa agctccatct ccataaacca ttacttctac atctttccct gttatttctt
    15121 ttatattacc ttggattttg tttacaactt catcacaatt tatagggaat aattttactg
    15181 tttcttctgt agctttgttt gaacctaaaa gtccatactg gtcattgtaa ccactaccat
    15241 ctacacttgc tgtcattata tcatcaagtg aatatacttt tttagctcca ttttgtctta
    15301 aaattctctt tgttctttgt cttgtatgta tatcacaagt taaaatactt gttgtatagt
    15361 taagaatagt ttttggattg tttgcaaata ctatttctac ctctgcacca caatccttga
    15421 ttaaattctt ataataatca acataatcta caccagtaaa tgtatgcttg ttatatccaa
    15481 atagttctct gtatttttct tctgttaaaa catctgacca aggatttatt cccttatcat
    15541 caagttcatc aatacttatt aagtgatttc caacttcatc agaagggtag cttaacatta
    15601 atacgacttt tctacatcct tttgcaattc cttttaaaca tatagcaaaa cgatttctac
    15661 ttaaaattgg gaatataacc ccaacagtat catctccaaa tttatctttt acatcttttg
    15721 caatattgtc aacgtgagca taatttccct gtgctctagc tacaacagct tctgtaacgg
    15781 caattacatc tttgtcccta acttcaaagt tttcactttt tgacgcattt aaaacagaat
    15841 caacaactat tttaactaag tcatctcctt gtcttataat aggtgctcta acccctcttg
    15901 aaacagttcc aactaatcta tccattatct aaaatcctcc taaagaatta atattgtcat
    15961 attagtacca tgtatattat aattcaaata taattataag taaaataaat aatactaata
    16021 tttgacataa ggagtaatta tatatgaata taaaacttga actttataag gtttttaatg
    16081 cagttgtaaa taataagagt ttttctcttg ctgctaagga attgtttatg tcacagccag
    16141 ctgtaagcca gtcaataaag caattagaag aacaattaga tactttatta ttttatagaa
    16201 acaataaggg agtaaaatta actccagagg ggaaaattct aagtgaacat gtcactactg
    16261 cattaaagtt aatatcatca ggagaagata gaataaataa atttaaaaaa ttagaatacg
    16321 gttctctgaa aataggagta ggagatacag ctgcacgttt tttcttgtta aaatatttag
    16381 aaatttttca taagaagtat ccacatatac atgtatctac aataaataga acgagtagag
    16441 aacttatatc tttactcaaa gatgggaata tagatatagc aattataaac atgccaatag
    16501 aagatgatac actaaatatt gttgagtgta ttgaaataca tgatatattt gtatgtgcaa
    16561 atgattacat agaatacaaa ggtaggaaaa tatcattgga agaattaaac acattgcctt
    16621 taataatgct agaaaataaa gcaaattcaa gactttatgt aaatgaatat tttttatcaa
    16681 aaggtataaa actaaacccg gatatagaat tgggttctca tgaattactt ttagaatttg
    16741 cgtatataaa tcttggagtg tcttgtgtta tagaagaatt tagcatagat tatttggaaa
    16801 atgagaaatt gtttaaatta gatataaaag agccaatacc taagagaaat ataggttact
    16861 gccatttaaa agatatatct ttatccttag caaccaaaga atttttaagc atgatatcta
    16921 ataatatata atttagaata catatttcac aagtaattta atataaaaat agagataaat
    16981 tcgtaaactt gaaagattca tatagtaatt agtttttatt aaaaataaaa ataaggttaa
    17041 tttgagaaca tttatataat taatatcgta ttttaaatat gaacagtata tttagattaa
    17101 tgtataaagg gggattagaa atgtctgatt ctgaatatcc tcaaatgaaa accttagaaa
    17161 ctagaagttg tatactaaga cctgcatcat tagatgatgt tgaagatttt tttgactgtt
    17221 ataaaaataa aatagtagta aaacatttac catttagtga gcataaatct ttagaagata
    17281 caagaaagtt tataaaatca ttttttctaa atccatataa aaaagggaaa attggccact
    17341 ttgctatagt atataaaaga gacaataaag ttattgggaa tatggggttt aataatataa
    17401 atcctaaagc attagaagca gaaataggta tatgtataaa tccaagctat tggggacatg
    17461 attttgctac tgagataaca aaaagagtaa tacagtatgg atttagagaa ctaaacttaa
    17521 ataaaataat tgcaataacc tatgaagaaa accaaaattc tacaaaatca ttagatttat
    17581 taggatttaa atgtacagga aagtatatca aaaaaattca tactggaaat actgtaaaaa
    17641 atgttccttg ttatcaatat gaattgaaaa aacaatagat ataatcattt aatatctatg
    17701 ctggttattt agtaatgatt gttttctaaa taaaatttca ataaagtggt tgcctcaaaa
    17761 tatagataat cctgttttta ttgttaaaat actgaaaatg cattttattt tatacaaaaa
    17821 agagtgtctc atgaactaaa aagtttatga gacactcttt ttgaataaat cgtattttaa
    17881 ttaaaatagt atatatactt aaaatattaa tttttattag ttctctaaat ttttaaatcc
    17941 ttctccaaga acctcatgta catctgtaac aataacaaat gcagtagggt cagttctttt
    18001 aactaatctc tttagataaa gttcttgttt tttagaaact acgactaata gaacttcttt
    18061 attttctcta gtgtatccac ctttaccatc aagaacagta agacctctat caagttcttc
    18121 agttatagct tgtcttaaac tttcaggctc atcagagata ataaaaaatg cttttgaatg
    18181 gtcaaatcct tccattatca tatctgctat ttttattaat acacaaagtg ctataccaga
    18241 gtaaagagca gtttctaaac tacgatttac tataccagaa gatataacaa ccattccatc
    18301 taggcatgac atgaattttg tagcttttat tcctggaaat ttcttactaa gtatgagtgc
    18361 aattaagtct gttcctccag tagaagcatc tgatttaaac attattccta aacctatacc
    18421 aactaggatt cctcctgcaa tagaagacaa taataagtca ttagtaaatc ttagttttga
    18481 taatgggtct gttaattgaa ctatagctga aaatacaact gttccaaata aagtttttaa
    18541 tgaatttttt gtacccataa ttctaaaagc aagtattact aatgggacac ctataattaa
    18601 catgatagct gatactggaa gtcctgtaac cttgtttagt actaatgata gaccactaag
    18661 tcctccagga gcaattgtat gtggttttaa aaacatattt ataccaatac tcattgatat
    18721 acaaccaaaa aatagtccaa gcatttcaag taaaattggc gacttttgtt ttttcatata
    18781 aacacctcca tgaacttgtc acaatttaat attataacat atttgaccag tttatattat
    18841 ataaaatttc ttgatataag caatttcaag tatttcatta ttcttaatat ttttgaattt
    18901 ttatatagtg ttttcatatc tttactgatg ttgttacact cctatgaagt tttttatata
    18961 ttataatttt tttattcttt tataaagtat cccctataaa gatggttcta tgaaaatttt
    19021 ctctatactg tttaacgtat atagagattt tgtgtataat tataaggtat ttgatggaaa
    19081 aatttatctt acttttatat gttatcaaaa tgatttatta aatacaacag acagcttgat
    19141 aacatataat tatataaata taatttatgt agatagttgt atttttaaat aaatttttac
    19201 atatcaataa ttattgtcta ttttatataa gcaacaatca aactaaaata aaagtttaag
    19261 aaaagagttg attaaatgca taaacctagt tataaaacag tatttcattc atacattatt
    19321 tttttcatga tttttatttg cttaatcctg acagtaatag gtattttctt atcacttatt
    19381 actattcaga aaccaaatgg tgaatttatt cgtagtgatt ggccgaaaaa atttacagaa
    19441 aactttaatg aacaaatcgt atttatagat aataagccac aagtaaaaca gtctggatta
    19501 gaaaaacttc gagaaaatca attgtggttg caaattttag attctagtgg aaatagagtt
    19561 tatgggtttt tagaaccaaa agagcataaa gattattatt ctaattctga attattagca
    19621 ttagctaacc aaaataattc aagtaatgga actgtatact ttaatacaat taaaaataat
    19681 aaaaatgact atatttatat tatatatttt ccattaaata tatctaaagt gaatatgttt
    19741 tttgatggag gtaaatttat aggtggtaag tcaattgtat tttcaatcat agggattata
    19801 tttttaataa ttttggtttc tggaattgct tatggttact gggtaacaaa gtgcatatca
    19861 aatattacat ctgctgttag cgatatttca aaacgaagct atcttccagt taatattaaa
    19921 ggtgtttttg ctgatatata taatagttta aatattttaa atgaagaaat tcatagaagt
    19981 gatgagatac aaaataaaac tgatattatg agagaagagt ggatttcaaa tataactcat
    20041 gatttaaaaa ctccactttc accaataaaa gggtatgcag agcttcttac agatagtagt
    20101 actaattatt cacctgaaaa agtatataaa tatgcttcaa tcatgttaaa gaacatagaa
    20161 tatacaaata cacttataaa cgatttaaaa ttaacctatc aattaaaaaa tggaataatc
    20221 cctttaaata aaaataatta tgatttagta agattcttga aagaattatc cattgatatt
    20281 ttaaattatc cagaatatga atcaaatata gttgaattta agagtaatat agataatatt
    20341 aactttgatt ttgattgtac actattaaaa cgagctttta gtaatcttat tgtcaatgct
    20401 tttacatatg gagataaaaa tactaaagtt tgtgtacatg taacattaaa tgaaagaatt
    20461 gaaatcatta tatctgataa tggaaaaggg atgtcaagag aagaagcatc aaatttgttt
    20521 aatcgttatt atagaggaag caatacaaaa caaaaaatag aaggaactgg tttaggacta
    20581 gcaataacga agcaaattat tgaagttcat ggagggaata tatctgttga aagtatacct
    20641 ggattaggta gctcatttta tatcaccttt cctatttaat taaggttaat taaggttaaa
    20701 taaaaaatat attaaggttg atagattatt atatttatat agtagtttat caatcttttt
    20761 tatttaaggg gtgatttgat taaaaatatt ataaatagaa tgataaaagt atggaggagt
    20821 ctattagaga tttaatttag attaaagtat aaaaatatta gattgaaata tcttagataa
    20881 gggaagaagg tgtaagtttg aaaattatat taaagtatat tttcacaaat attaaagaac
    20941 gaaaaattag aacagcagtt atgttattgt ccatagtttt atcaacagtt ttactttttg
    21001 tttcattctc aattggatta tcttatgaaa gtgcacaaag aaaaatggca aaaggtatgt
    21061 atggtacagc gactatatct gtccaaagta aaaatccaga tatattaacc aatttagaag
    21121 acatacctga tttaaatact ataaaatcaa aggttggtgt attggaaagc tctgctattt
    21181 ataataaagg gggatactat gaagaattta gtcttatatc agcagactta agtcaactaa
    21241 ataaaatcaa caagccacga ttagaaaatg gcgatagtat tactgatttt tctggtgata
    21301 aaattattct tccaaatcgc tttacatcaa aatataaaat aaaaaaaggt gattctatta
    21361 ctttgcagat ttatggaaag tcttatactt ttcaagttag tgatattgca tcttatgata
    21421 cagtattttt gaggaacaca agaggtgtaa atgcactgct acccaaagag actctatcaa
    21481 aaattataaa taaaggcagt ggatatacaa gaattcttat agagtcagaa gaagaaatta
    21541 cagaaaatct agtaaataaa ttatccaaag aactttctac tgaaaaatat atggtatcaa
    21601 atactataaa tgaaactaag ataatttcag atgctagaca aaagacaatg cctttctttt
    21661 taataagttt ttttgcatta acccttagta tatttataat atacagcagt tataaggtta
    21721 ttacattaga acgtcttcct tttattggaa catttagaag tattggtgcc aatgaaaaaa
    21781 ctgtaactcg tatattgatg cttgaaagta tattatacgg aagtatagga ggattaattg
    21841 ctattcctat tggtgtagtt gttttaaatt taatgcttca tgggttagga aattctctcg
    21901 aacaaggaat atcaattcca gtagtaatat cacccattgg agtgattata tctgttatag
    21961 tagcaataat cgtttcttca tttagtgcat atatacctgt taaaaaagct agccatttgc
    22021 caataaaaaa tattgtacta ggaacagtag aagaaaagaa tgtttcaaat cgttctatac
    22081 tttttatagg aagtataatg tttattttat ctattttatt tccaagaata tcccctgaaa
    22141 atacattgta tctagctggt ggattttcat tactgggatt aattgtagct actattgtgt
    22201 tgattcctct tattactgat atcatgtcta tagtttttga gtttgtttat aaaaatattt
    22261 tagggaatga gggtaaatta gctgctagaa atatgaaaaa taacaaaaat ataatacaga
    22321 atatcacttt actatttata agtatttctg cagttattgc aataagtgtt gttggtaact
    22381 ttgtcaaaac ttatataaca gatgtgtttc gtgacgcaga attacaaggt tttgcagatg
    22441 gaaagatgaa tgaagagttt attgaagatg ttagacatat ggatggaatt aaaaaaatat
    22501 tacctcttta tgttatgaat aatgagattt ctggtaatgg tgtaacatta tcaagactag
    22561 agggtacaga taatataaaa ctatataact ctatgtttgg aatcaattat acaaattttg
    22621 agataaaaaa acaagtaata gaagctttta atgataaacg ttctgttatt ttgaatgaag
    22681 atactctaaa aaaagtaggc ttatctatag gagatacaat aactctatct aatgataagt
    22741 atgatttttc ttataaaata gtaggtagtt ttaaatcccg tgcaaatgat gtagaagctg
    22801 ttataccatc acattatgca gttaatgatt ttgataaaac taattatggt tttttagtat
    22861 atacagcagt caatccagat gctgttatga ttcagataag atatttattt ggagatacat
    22921 ataattggag tcgtacagtt gaagaattta ataatgattc tttaaatact ataagtagtt
    22981 ttttgtctcc tatgaataag atgacctatt tcatttttct tcttgctaca gtaggtatta
    23041 ttaataatct actgattaat tatattcaaa aaagacgttc tatagctatg tataaatcta
    23101 taggactaag tgataaacaa aatataaaag taacattgat tgaagggttt acttcaggtt
    23161 tacttggagc tgttattggt attgtgatat ctatacttga gattcaaact atctttatag
    23221 ttgcaggacc aaaaatttca atgaaaccag atttagattt taaaacattt attattgttg
    23281 ggttattggg aattatagta actttaatag gttcgatagt gcctattata aaagggaaga
    23341 aaatgaaact gatagaagaa attaagtttg aataattatg gaggttaata tgaaaattac
    23401 acataatagt ttagctgtag aagcaaaaaa tattatcaaa gaatataaaa ttggaaatac
    23461 aacaactcga gtgttaaaag atgtatcttt acaagtaatg aaaggtgaat ttgtttctat
    23521 catgggtcaa tctggttctg gcaaaagtac tcttttatat attttaggtg gtttagatac
    23581 tccaacgagc ggaaaagtgt atatgaatga agctgatata tcacatttta atgatgagaa
    23641 aatgagcata atacgtcgta gaaatatagg attcgtattt caattttata acttaatacc
    23701 aaatttaaat gtggaggaaa atataatgct tcctttgtta ttagatggta aaaatttaaa
    23761 agattataaa aatcaattag atgaaatctt agatatagtt ggtttaactg atagacgtaa
    23821 acatacacca cgagagttat ctggtggtca gcaacaacgt gttgctatag ctcgagcttt
    23881 aattggaaaa ccagagattt tatttgctga tgagcctact ggaaatttag atagtaaaac
    23941 agggatagaa ataatagatt tactgaataa aataaacaga gacaatggac aaactataat
    24001 tatggtaact cactcaccag aagcagccaa aagtagtagt cgtactatta tagtttcaga
    24061 tggtttaatt gtataaggac taattattgt atttgagata agcattttta attatataaa
    24121 ttagacatat gtaaagtgga tataagttaa atactataag tgtgctataa tattaaaata
    24181 acagataaaa gtactatatt ttttattgat tattttaatt ttatttgttt attaaatatc
    24241 taatgagtat tcatactatc tctattatta aaagcagtta atgatatata gagataatat
    24301 ggattcttct tactttaata aatattcacg ataggacagg atataatata tgttaaaaaa
    24361 agaaagtatt cattttataa ataaaaatag gtcacttata gtttgtgaag acaattttgt
    24421 ttatctcttg ccttgtatac aattaagaaa ttggatatcg aattttacta tatcgtttcc
    24481 aaatcaaaca ataatttctg ataattacac gattattcct catggaagtg taactttggt
    24541 gtttttttat gatgccacag ggttacatag tctattattt ggtccaacta ctaaaccaaa
    24601 aacagtagga aatattgcta atagatgtga tgttatacta attatagaat ttcaacctgc
    24661 tggttttttc ccgcttatag gtatacagca aagtgaactt attgataaag ttgttccatt
    24721 ttctataatt aatacttcat tagatttaga aattaaaaaa atatttaatg aatctttatc
    24781 tatagataaa ttgattttaa agttggaaga attattgatt tctaatatta aaattgaata
    24841 ctcatatgag tttattttgg caattcagtt gattattcaa aactctggaa atatatcttc
    24901 acaagaaatt tctaaaaaag tattttatag ttctaggcat ttaaacagac tctttaatca
    24961 gtacctaggg ttgagtatga agtcattttc tcgtttggta cgtattaata aatctataaa
    25021 acttctaaac aataataaaa cttcattgat gtctatatgt aatgaattag gattctatga
    25081 tataccacat tttattaaag attttaaaat tgtctgtgga ataactcctc aaaaataccg
    25141 agctaatatg tctgattttt acagtgaaat tgcaaaattt taagttataa tacttataac
    25201 aaagaaagga tagtgataag atgaaatttg gaggagtatt gctaattgtc aaagacataa
    25261 ataaatcaaa aaagttttat gaagatgtta tggagcaaaa aatcacatta gatttaggag
    25321 aacatgtatc atttgaaaat ggatttggat tacaaagtaa ttataaagct ataattggat
    25381 atgatttacc aatatatcaa caatcaaata actttcagtt gtattttgag gttaatgaca
    25441 tcaaacaatg ggaaagtaag ataaatagaa taggaaatat tgaatttttg cattatataa
    25501 aagaataccc atggggtcaa agaactttta ggttttatga ttttgataaa aatattatag
    25561 aaatttcaga aagtatggaa agcgtcataa agaggctttt aaaacaaggt ttagctttag
    25621 aagaaatttc aaaacgcact atgtatccag ttgaatttat tacacaattt caacaattat
    25681 aaaatattaa aatatataat tttttcttta tattacaaaa aacaaaggct tggggcataa
    25741 tagaaccaga aaaacctgaa tctcaacagc aaatggctca tatggatttc gctgttaatg
    25801 atttagaaga agcagttcaa tatgcaattc attgtggagc tacaatcgca gaggagcaat
    25861 ttactgatga ctggagagtt atgattgacc cagctggaca ccctttttgc ttatgtcaga
    25921 tgaaatcaat tatggaaagt agccattttt cattgttata gaccaaaata ttgtttatta
    25981 attacaaaaa agcacaacag tatataccgt tgtgctttta cttattatac atgaatacac
    26041 taaaaaatat aaatagtaga gctttatcaa taatgataat ttttaaaaaa tagtattttt
    26101 ttattgaata atttcttaga tattttaaag tatataaatt ttgcatatat gaattggaca
    26161 ttatcaaatg tgttataata ttaaaacaac aaataaaatt attatatttt ttagaataat
    26221 aattttattt gtttactaag tttatgatag ggtatttatt taaggtaata aaatattata
    26281 taaaatattt taaagcccta ggtaatatta ggtagaagtt ttagtaaaat atgcaaaaac
    26341 taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa aatatttgaa
    26401 agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt taaaaagtat
    26461 cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag gtgttggttg
    26521 tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact taacttatga
    26581 agttggagag ttacaatttg taatgagctc tgatgaatat agtcagtatg gagatataat
    26641 aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaaag atcaaggtgg
    26701 aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta agtttagaac
    26761 tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt ggtgagataa
    26821 tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat tgtgagaaga
    26881 agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata caaaatatgt tttaagagac
    26941 gatatagaca aattaaaagg caagtggaat ttaaagttta gaaatgataa tccaatacat
    27001 gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca gaaccctgac
    27061 ataaattata ttgccatgga acttaaggaa gaagttcttt taaaggctgt tgaaaaagca
    27121 gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa tattttggat
    27181 tactttgaag ctaaagaatt atcgagaatt tatataaatt tctgtgaccc atggcctaaa
    27241 aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta caatagggtt
    27301 ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt cgagtttagt
    27361 ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga tttaggaaat
    27421 agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc acaaggtatg
    27481 cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa gtttaatagt
    27541 agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata taacaaggaa
    27601 acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg gagggtacta
    27661 tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt ccaataaatt
    27721 caaatgataa ggttttgagc gaaaatcaac ttaaagatat ttttcctgat gcaaatttaa
    27781 gagcagttgt aaaaagatat ataaacccag atgagatgac tataagcaat ataaaggctt
    27841 tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaaggt atatcatatc
    27901 ttgaaaatgt agacaatttt attttttgga ataataatat aaaagaggtt cctaaggaag
    27961 ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg attgatgacg
    28021 atgtagtaaa ttctctttct cataatggtg tggatgtaaa ttgcgattta aattttattg
    28081 atacaaaaga taatcagtat aaactggact ctaaatatca taatgttgat atagcaaaag
    28141 gtgaaagtat tgatttgaga aaaataatta cgaagaaaat tgatagttat tataaatact
    28201 gggaagttac tgataacctt ccaaaagatt taaattttat tgtatctgta agtgataaga
    28261 gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa gcagtggtaa
    28321 aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta attactgtca
    28381 atgtaaaata atctgtcaac ttattcaatc attctatttt caatttaagt gtataatcaa
    28441 aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc aacctttaaa
    28501 ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc catattccta
    28561 ttaaaattat cagtgaatat attatataca aaactattct tatatattgg acatctaatc
    28621 caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca gatataccaa
    28681 taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt ttacccatcc
    28741 aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta tctaccaaag
    28801 cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa aaccctacaa
    28861 acaacttttg aatccataat aagataactt gattaagcac tatgaaataa gtactataaa
    28921 acttcatcta cattaatata tactacacta tacttcaaat ttcttaaaca taaattaatg
    28981 aaattaacaa aattttattc agaaacatat aaagatatat tttactattt aaatgcatat
    29041 cattatatat acagttttag gaggtgaggt atatggaatc taaatttttt aattggtaca
    29101 ctcaatcctt agggggaact tttggcatta tatcatgtat atgcgcatat ttaaatggat
    29161 acatgtttgt ttatacaaat agtgctagca aatattttga aagtatagga tttctgggaa
    29221 taatttcaag ttatacacta cttccacttt gtatgcttac tttgattttt ggagttatat
    29281 attcttatac tccagaaaca tccgacaaaa gtatttttaa tatacctttt tttactttaa
    29341 ataaaagatt aatacttatt acagtagttt ttggttttat aggtgctaga gtttacttta
    29401 taattcctgc aattctaata acttttaacc tatatagcaa tgttttgata gctaaattaa
    29461 agtacatcct atgtaaaaat aaagaaaatg atactaattt gaaagaaagc gaagtctaaa
    29521 ttgcataata ttggtatttg tcttaaaaat tcccgaggta ttaatttgta ttctcttgta
    29581 ttttgcaaaa aatattgaat tttattcatt ttatacttat aatttatatt aataatggaa
    29641 aattaaaaca aatataagtt actggttgtt aatttaaaat taaaagatat attgatgtgt
    29701 tgattctcta gtcagcactt tttcttttat aaatttgttt tttgtgatat aatattagca
    29761 ccaggtaata atacaagggg gtaacaaaat gaacatcaat gaaattaagg aattattaaa
    29821 ggccatagac tcaactaact tagaatatgt aaaacttgaa agtagcgatt taagattaga
    29881 agtatctaaa aaagctcaat ctacaagttc acctgtttta agtgtacaac aagaatcagt
    29941 agtggatctt tctttagaaa agccagttgt aaacgataca cctgtcacat ctaatgagaa
    30001 tctatcagta gtagtagctc ctttgatggg aacattctat gattctccaa gtccagatgc
    30061 agatagcttt gttaaagttg gcgatgtagt tgaagaaggc gacactttat gtatattaga
    30121 agcaatgaag cttatgaatg agataacaag tgaaattaaa ggtgaaataa ttgaagtatt
    30181 agttagtaat gaagaactag tagaatataa tcaaccttta tttaagataa aaccattgta
    30241 gtaggaggta tttaagttga taaaaaaaat actagttgca aatagaggcg atatagcagt
    30301 tagaatcatc agaacatgta aagaactagg tataaaaact gttgctatct attctgaaat
    30361 agataaagat tgttttcata gatatatagc tgatgaatct atttgtatag gaccaaataa
    30421 tataagtaag agttataata atatagaaaa tataatctat cttgctctta aattaaagtg
    30481 tgatgctatt catccaggct ttggattttt atcagagaat cctgaatttg caaagcaatg
    30541 tgaagataat aatattattt ttataggtcc aacaagagaa caaatgattt tgatgggaga
    30601 taaatctaga gcaagagaaa ctatgatgga attgaatatc cctgttgttc ctggttcaga
    30661 atctgttttg aaaactaagg aagaagcttt agaagttgca agagaaattg gatatcctgt
    30721 tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg agaatcgtta gaaaagaaga
    30781 agaacttttt tcaaattttg atatggcaag ttcagaagct ctagcagcat tttctaatag
    30841 tgatttatat atggaaaagt ttatagaaaa tcctagacat atagaagtac aagtatttgg
    30901 agataaacat tcaaatgcaa tacatttagg tgatagagat tgttctatgc aaaggcgaaa
    30961 tcaaaaagta atagaagaat ctttaagtcc atacctttca gatgaagaaa gacaaaaact
    31021 tcataaaatt gcagtagata tagtaaaagg agtaggttat atcggtgcag gtactattga
    31081 gtttatagtt gacaaagata aaaactttta ttttatagag atgaatacaa gaatacaagt
    31141 tgaacatcca gttacagaga tggtaacaaa tcttgacctt ataaaacttc aaatatctat
    31201 agctaatggg gataaaattc cttttaaaca agaagatatt acttttagag gtcatgctat
    31261 tgaatgtaga ataaatgcag aagattcatc aaagaatttt gctccatcac caggtaagat
    31321 agaatcatta aatttacctg gaggttttgg tgttagattt gatacttttg tatatgcagg
    31381 atatactata ccacctttat atgattctat gataggtaag ttaatatgtt gggctgaaac
    31441 tagagaagaa tgtataagta gaatatatag agctctagac gaaattattg tagaaggtat
    31501 taatacaaat gtagaatttc aaaaggcact tgtaacaagt gaggagttta gagaagatac
    31561 tcatcatacc aaatttatag aagatgtctt tatgaagaaa gaatttgcta cattataagg
    31621 agtaaataga ttatgataaa aaaattttta tcatcaaaag aatctaagta tgttacgatt
    31681 tctttagatg acaactttaa aaagaatagt gtagatgata aattttggac ctattgcaaa
    31741 ggttgtgata gtcatgtatt tagaaaggac atagaagaaa attcatttgt atgtccaaaa
    31801 tgttcaagac attatggctt aagagcaaga aaaagaataa atttattgat tgataaagga
    31861 acttttatgg agtttaattc tgatgtagaa tttcaaaatc cactaaattt tcctaaatac
    31921 aaagaaaaag tagactctta taaggaaaaa acaaaagagt cagaagctgt tgtcacaggt
    31981 tatggaagaa taaatggaat taaaacagtt atttgtgtaa tgaatcctga ttttatgatg
    32041 ggaagtatgg gttctattgt tggggaaaaa ataacttatt caatagagta tgctgctgaa
    32101 aataatttac ctataattat ttgttctgct tcaggaggag ctagaatgca agaaggtatg
    32161 gtatctctta tgcagatggc aaagacttct caagcacttt ctaagcttga agaaaaatca
    32221 ttgccatata tatcagttct aactgaccct actactggag gagtaactgc tagttttgct
    32281 atgcttggtg atattataat ttctgagcca aatacgttga taggttttgc aggtccaaga
    32341 gtaatagagc aaacaataaa tcaaaaactt cctgaaggat ttcaaacttc tgagttttta
    32401 cttgaaaaag gttttattga catgatagta gatagaagaa aaatgaagga agtattatat
    32461 caaattcttg ctatgcacaa gaaatagggc ataaaggagg aactgttttg attgaaaata
    32521 atagtgaaaa aataaaagtc ttagaaaatg atattaaaca attaataact atttctaagg
    32581 caaataatat agatttaagt gataaaataa attcacttaa tgagaaattg gaaaagctta
    32641 aggaagatgc tttttcacat ctatcagcat atgaaaaggt aacattaagt agagatataa
    32701 agagacctac gacattggaa tacatagaac atatttgttc taatttttta gaacttcatg
    32761 gagataggct ttttaaagat gacccttcta tggttggagg aattggtcag ataggaaagt
    32821 ttaatgttac tatagttgga caccaaaaag gaagagatac aaaagaaaat ataaaaagaa
    32881 attttggaat gcctcatcca gaaggataca gaaaagcatt aagacttatg aaacaagctg
    32941 aaaaatttaa tagacctata ataacgttta ttgatacatc aggagctttt tgtggtctag
    33001 aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa cttacttgaa atgagtaaac
    33061 tttctgttcc agtaatcact tttgtaattg gtgaaggtgg tagtggagga gctttaggta
    33121 tcggagtagg aaatgatgtt tgtatgttag aacattcagt ttattctgta atttctccag
    33181 aagggctttc tagtattcta tttaaagatt catctaaagc taaagaagct tgtgatgtta
    33241 tgaagcttac aagtaatgat ttatatgatt taaaaataat agataagatt ataaaagaac
    33301 ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa agaaataaaa gcttatattt
    33361 tagaaagatt aaatcattac aaagatatgg ataaagagga aattatagct caaagatata
    33421 aaaaatttag aaatattgga aaatgcttat agaatataaa gaaatttgta gttttttcat
    33481 aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga ttatagaagt atatttttat
    33541 aaaagtatac tttaaatgat gattgtaatt agtaaatggc aattatgatt tagtacttta
    33601 ggtaatggat actttaacaa attaacaagg agatgttgaa atggaggtac taaaagtttc
    33661 atcaaaatct aatcctaatt ctgtagctgg agccttggct ggagtactaa gagaacgagg
    33721 cgttgcagaa atacaagcta taggagctgg agctctaaat caagcgataa aatcaattgc
    33781 aatagcaaga ggttttgttg cacccagtgg aatggattta gtttgcattc ccgcatttac
    33841 tgatatagaa atcgaaggtg ataagaaaac agccattaaa cttataattg aacctagata
    33901 atatatctct ttattagaat ttaattaagt tttaaaggtt ggctatagaa ataggacact
    33961 atagtgaacc ttttttactt tatatgtttt attttatgta acttttaatg tataatctta
    34021 agtaaacaaa taagattgga ggaaatatac attatgaaat taaaattatt ggtagaagag
    34081 tatgcagtat gtagattaaa taatgacagt aaaataccaa catggataga tacagagaag
    34141 ttttactcta taactagaac tgatgatgaa ttaagtgttg tatgtttaaa taataatatt
    34201 ccttctgatg tcaagtcaga aaaggagtgg agaatactta agatactagg tcctttagac
    34261 ttttcacttg taggaatttt atctaagtta agtagtttgt tagcagacaa caaaataagt
    34321 atatttgcaa tttctactta tgatacagac tatatcttag taaaagaaaa agatatagaa
    34381 aatgcttgta aaattttaag ctgtaatgga tatgaagtag agtagttaaa tacttttaca
    34441 tgaggactta acttatgatt tatataatcg aaattttaaa aataagtgta ataaatattt
    34501 tttgtttcat aggaatattt attgtatttg gattgatttt tagcattaca gaaaatctaa
    34561 ataataaatt tatcttttca tcttttggaa aaactggaat tattgtaact ggagctatag
    34621 gaacgtttgt tcatgaattg agtcatttga taatgtgttt gattttcatg cataaaataa
    34681 attcagttaa gtttttcaga ccaatagaaa gtaaaaatga tggtatacta ggatatgtaa
    34741 gtcatagcta taaaaaagat aatctttatc aaagtatagg aaatttcttt ataggaattg
    34801 cacctattat aggtggaaca ataatgatta ttttaatttt taagatatta ctgccagata
    34861 gttatatgaa agtgactcaa aatatagatt taaagttata tgtaagtatg attaataatt
    34921 taaatataaa tgggtttata cacattattt taggtgatat ttccaatttt ataagaataa
    34981 tgttgttaac acctagtgta tattcattga agtattttat atttatgttt ttaatgtatt
    35041 ctatatctac acatatgtca cttagtagtg ctgatttaaa aaatagctta aatggtctga
    35101 gctttatagt tatagttatt tttgtaataa gttcaattac atacttattt ggatttgata
    35161 acttgaatat atccagttta atagttaagt ataacatatt tataagtttc tttatggcaa
    35221 tagggtttat tttttcaata gtaactctca taatatccta tattttaagc ttagtcagcc
    35281 catttaaaaa ctttagttaa ataaatacat attttactaa tatttggttt cgttagaaac
    35341 atataaaaat attatataaa aaagatttta gtagagcata aaaaatttct caatatattt
    35401 taaaatattt ctataaatat attgagaaat aaaaaagagt acttaaattc aactagtctt
    35461 taaatgtgga tttagggaat agttagtgat aatttcatct aaagcactgc taatctctta
    35521 aaaatgtaaa aaatgattaa attaaatatt tatactcact tggtataaat atcaaaaaat
    35581 aatataaaag atttatattt tagttttagg aggtttaatt ttgaataatg ataagaaaat
    35641 tataaaagta cataataaat cagataaacc tgataatatt gttatcaaag aaaatgaact
    35701 catagaaaaa tgtctacttt tggaaaataa acttcctgta tttatgaagg attattttat
    35761 atatttaaaa ggttctgttg cagtttctac acgacttgct tatttagaag atattaattt
    35821 tttttgttca tatttgattg aaaccaaaga attaacaaat gcagattgtg taaaagatat
    35881 tacagaagat gattttaata caataaaatc aagagatata aatttattct taggtgatta
    35941 ttgcagtaga tattacaaaa atacagaaaa aaacacattg atttttgaaa ataacaatag
    36001 agctttagca agaaaaaaat catccatctc tactctattt aaattcttat acagaaactc
    36061 tcagatagat aataatataa ctgatggatt taatccaata aaacttccta aacctcagcc
    36121 agatgctatt aagcgccttg agatagatga agttgctaag atgcttgaat cagtagaaac
    36181 aggtgaaggg cttacagaga aagaaaaggt atattggaga aaaactaaat tacgtgacaa
    36241 agctatacta gcattatttg ttacatacgg gcttagatta aatgagctta gagaactcaa
    36301 tatctcctct tttaattttt caagaggtga atttaaaata tatagaaaac gtgggaaaga
    36361 agttttaatg cctataaatc atacttgtga acatgtaata aaagattatt tgcaaaacga
    36421 aagaacaaga gatgaactat taaatgatga agaaaaagat gctttatttt tgtctcttca
    36481 aaataagaga attacagcaa aggctataag aacactggta aaaaaatata catctattcc
    36541 acttgatact actagagaca atggatatag tccccataaa ttaagagcaa cggctgcaac
    36601 ttctctaatc caaacaggat tttctatata tgatgttcaa aacttacttg accatgataa
    36661 tgtcacaact actcagcttt atgcagctca taagaaaaat gtaaaacgtg atatagtcaa
    36721 gaattttgaa tggattgagg acgattaaaa atcaggaaca aatgtttgca gatataaatg
    36781 gattatgtta taattaaaaa tataaagtgt atggaaataa aaacaaaata tgtttggaaa
    36841 tgagtgatgt tatgtattta gatctaactg aaaagcaagt cttgatattg gaatttataa
    36901 agtctcaaat catattaaaa ggttatccac ctgctgtaag agaaatatgt actgctgtag
    36961 ggttaagatc tacttcaact gtgcactctc atctaaataa acttgaaaaa ctgggataca
    37021 taagaaagga ccctactaaa ccaagagcta ttgaagtttt agaacgtagt aaagtaaatg
    37081 atgtttctgg agctaatcaa gaaataatag agcttccttt agtaggtcaa ataacagctg
    37141 gagaacccat tttagctcaa caaaacatag aggaatacat tccattccct gccagtttag
    37201 taaagggcag taacaatttt gtattaagag taaaaggcga aagtatgatt aatgcaggaa
    37261 ttttagatga agattacgtt gtagtagaca aaaaaaatac agccttaaat tctcagatag
    37321 tagttgcact tataaatggt gaatctgcta cagttaagag gttttttaaa gaaggaaatt
    37381 taataagact tcatccagaa aatgatttta tggaaccaat tatgctcaat gactcagaag
    37441 ttgaaattgt aggtattgta actggtgtat ttagagttat taaatagcta ttaaaaaata
    37501 cttaaaaaaa taagctattc gtatagataa aatgtgttat ctattgatta tagcttattt
    37561 ttttatgcat ttattttatt ttttaaatga tttaaagttc agaagcttta tagataaatt
    37621 ggttgttttt ttgttatttt ccataacata tatcacatgg gtcatttttc ttactttctg
    37681 caattgtgcc taataatatt tttttacttc ttgaaagccc actacaattt tttgtagagt
    37741 gataactctt accttttgga gtaaaataca ctatttcttc tgaattggat gtattttgtt
    37801 tatttttaaa agaatcattt ttacttgtat tactgtttga ttttccagag tttccatctt
    37861 ctctaacgcc tttattataa cttccatctt tacaacttgt aaatactcca tcacctgttg
    37921 attcaaatat tatatctgaa cactcatcac ttctatgtac ttttactccc atttttttta
    37981 atttattcat tgtctcctga tgtggatgac catatgaatt acccttgcct actaaaagaa
    38041 ccgcatattc aggatttact ttatctaaaa actcttgaga tgaactacta cgagaaccat
    38101 gatgagctac ttttaaaagg tctactttct caagcttagg tagtatttct ttttcggtac
    38161 cttcttcagc atcacctgta aataaaactt tatcattccc attagtatat acaaggacta
    38221 gagattgttc attagtagta tctcccccat ttgtatttag aacttcaaaa taagaatctt
    38281 ctaaatagaa cttattattt tcaagcggta cacttgggga taaaccctta tttgctaaag
    38341 catttataaa gtccctataa ctctttgttt ctgctgaacc attcgataca aaaacatttt
    38401 ctatattaaa gtttttgaca acagaatcaa gtccacctaa atggtctgca tgagaatggg
    38461 tagctataag atatttaata tcttttacac cttgattgtt taggtaatct accattaatc
    38521 cctcatcatc attatctccc ccatctatca atgtaaaagt tttaccatct tgaatcaata
    38581 ttgcatcact atttccagtg tcaataaaat gaatttttac attcttatca cttgatatac
    38641 tactagacaa agaatcatta ttatttgatt gattatttcc attatctgat gtagtacaac
    38701 cagttaaata aatcgatata gatactatca ataaaagtat caaagtttta attttagaat
    38761 tttttttatt ttttatactt atttctatca caaactacgc ctccatattc ataatactct
    38821 agtaattata acatgatatg cgtttttatg atattttttt acataatatt ttaaataata
    38881 gattatagta caataatata gaaaattgac actgtataat ttttatatta tataaaatga
    38941 aatattttac atcagtagta tccagttatg cattttaaac gaccatttac gtttaatata
    39001 ttgcaaactg cttctaaaaa ctataatatg ttaagtatca acaaaaataa ataaactttt
    39061 tccttaaaga aggcttaaag ctcacaccaa ctgccttctt tttttttata aaaatttaat
    39121 tatttatttt gattaatatt tatttcgtat tttagttcta ctcttctaga tgcatctcta
    39181 ttcacgctat tgtcaccatt tttgatgagt tcagcttttg accttcctat tgcaataata
    39241 tgcttagtca ctttatcttt gtatttatag tcacctattt catctcccac tataaattta
    39301 gcaacgctat atgctcttct ttgcgataaa tctaagttgt atatataact tcccacatca
    39361 tctgtatgac cttctataac tattttggat atatagtcac cataatcttt ataaatagtc
    39421 tctatgtact ttggaacaaa catttttagc atttctttag cctctggttt taaaacatca
    39481 ctatctacat caaacatggt tttttcacca aaagttactt gcccattaga tttatctacc
    39541 ttcacaggta ttccactttt tttaaaagtt tcttcaacag aagcattgac attcttagct
    39601 aaatcttgtt caacagaacc tgaaatactt tctgagctaa ataagataag aatcacaacc
    39661 atcaatacag tagataataa atctgtaaat gttggccaaa agctactctt ttcaaattct
    39721 ctgttaacag ttcttctata tttattattc atttaactca tcctcaccta tgacctttat
    39781 ttctttatcc tctatctcat aaatttcttc actaaaagga ttaaatacat tattaacatc
    39841 atccttaaat aattcttcac tatcaagagg aacttcatca ttcataaaag agttattaaa
    39901 cataacatct aacttatctt gaatatctaa agaattttca aacatcatat ccagtttaac
    39961 ttgtaggtca ttagaaaagt tttcaataat acttgagaga ttttttattg agtcatctaa
    40021 ggtaaacttt tcatctaaaa ggattttttg attttgagag attttatttt catgggtcat
    40081 taatttagtt aataagtctt gagcacttct ttcatataga gcaaatcctg catttatatt
    40141 ttttatataa tctctaattt tgaattggct ttcagtggca ttgtatatat tttttgaaga
    40201 ttcttttata tcaaataaaa tctctttatt tatattatct tgattatcat atttatcaaa
    40261 aagctttaca aattttatat ttaaaatatt aaactgttta tctaattttc ttacgctctg
    40321 gtcaaatgtc ttaatagaac tttgagaacc ttctaaaagg ctttttaaag cttctatacc
    40381 actgataaat tcatcattaa agtcatttaa tctttagata ttttatcaaa tttttcaata
    40441 gcttctatag acttacttat ttgttttatt gtatttttta cctcttctac ttttaaatcc
    40501 attctttctg acttaaaatg agttatttca gatgttagta aattttcaga ttttaacatt
    40561 atttgtatca aaacatgttc acaatctttt atcttagtaa agtaacctat aattagagaa
    40621 aatacaactc caaatatact agttgtaaat gcagtttgca tactactaat agttgaagga
    40681 agtgaattta taatgtcttt agtatcaaca cataaaagca ttgtagaaag accaacaaac
    40741 gtacctaaaa ccccaagtaa tatacttatt gaagatgaat tttttatact tctaattttt
    40801 tctaacaatg gcaaattatt atgctttaaa tcagatacaa cttcctctat aaatgaagtt
    40861 atatttacgt ctgcataagg attttcttta ctatatgtat agtagtcatc ccttaccttt
    40921 ttataaaact cacttgtctt atagtctcta ttgatttcgt ctaattttga acttacaaat
    40981 gaatatactc tgttattttc tattatttta aaaacagaat acacagttat tgcaagtaaa
    41041 aacaatatag ttattggatt agtaaatatt gactctatta aattcgttat tatgttactc
    41101 aaa
//
LOCUS       DAFKMX010000010        40301 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFKMX010000010 DAFKMX010000000
VERSION     DAFKMX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.010"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            476..1498
                     /locus_tag="LZB08_000436"
     CDS             476..1498
                     /locus_tag="LZB08_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBM7313956.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANANQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            1794..2714
                     /gene="pyrB"
                     /locus_tag="LZB08_000437"
     CDS             1794..2714
                     /gene="pyrB"
                     /locus_tag="LZB08_000437"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBM7313957.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2753..3445
                     /locus_tag="LZB08_000438"
     CDS             2753..3445
                     /locus_tag="LZB08_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBM7313958.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3438..4340
                     /locus_tag="LZB08_000439"
     CDS             3438..4340
                     /locus_tag="LZB08_000439"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBM7313959.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4425..5009
                     /locus_tag="LZB08_000440"
     CDS             4425..5009
                     /locus_tag="LZB08_000440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBM7313960.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5224..6150
                     /locus_tag="LZB08_000441"
     CDS             5224..6150
                     /locus_tag="LZB08_000441"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBM7313961.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERN
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6202..7092)
                     /locus_tag="LZB08_000442"
     CDS             complement(6202..7092)
                     /locus_tag="LZB08_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7313962.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7280..8311
                     /locus_tag="LZB08_000443"
     CDS             7280..8311
                     /locus_tag="LZB08_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBM7313963.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            8605..9480
                     /locus_tag="LZB08_000444"
     CDS             8605..9480
                     /locus_tag="LZB08_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBM7313964.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9498..10988
                     /gene="cls"
                     /locus_tag="LZB08_000445"
     CDS             9498..10988
                     /gene="cls"
                     /locus_tag="LZB08_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBM7313965.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11181..11465
                     /locus_tag="LZB08_000446"
     CDS             11181..11465
                     /locus_tag="LZB08_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBM7313966.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11498..13126
                     /gene="groL"
                     /locus_tag="LZB08_000447"
     CDS             11498..13126
                     /gene="groL"
                     /locus_tag="LZB08_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBM7313967.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            13388..14128
                     /gene="larB"
                     /locus_tag="LZB08_000448"
     CDS             13388..14128
                     /gene="larB"
                     /locus_tag="LZB08_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBM7313968.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14212..14946
                     /gene="larC"
                     /locus_tag="LZB08_000449"
     CDS             14212..14946
                     /gene="larC"
                     /locus_tag="LZB08_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBM7313969.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            14976..15395
                     /locus_tag="LZB08_000450"
     CDS             14976..15395
                     /locus_tag="LZB08_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBM7313970.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15455..15556
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15777..17312
                     /gene="guaA"
                     /locus_tag="LZB08_000451"
     CDS             15777..17312
                     /gene="guaA"
                     /locus_tag="LZB08_000451"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBM7313971.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            17851..19281
                     /locus_tag="LZB08_000452"
     CDS             17851..19281
                     /locus_tag="LZB08_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7313972.1"
                     /translation="MILNQIFIEVGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVSGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19429..19779)
                     /locus_tag="LZB08_000453"
                     /pseudo
     CDS             complement(19429..19779)
                     /locus_tag="LZB08_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            19774..20295
                     /locus_tag="LZB08_000454"
     CDS             19774..20295
                     /locus_tag="LZB08_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBM7313973.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIVGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(20424..20660)
                     /locus_tag="LZB08_000455"
     CDS             complement(20424..20660)
                     /locus_tag="LZB08_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313974.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     YKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(20970..24398)
                     /gene="uvrA"
                     /locus_tag="LZB08_000456"
     CDS             complement(20970..24398)
                     /gene="uvrA"
                     /locus_tag="LZB08_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBM7313975.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDIGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            24717..26951
                     /locus_tag="LZB08_000457"
     CDS             24717..26951
                     /locus_tag="LZB08_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7313976.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            27207..29237
                     /locus_tag="LZB08_000458"
     CDS             27207..29237
                     /locus_tag="LZB08_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7313977.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAKKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            29234..29686
                     /locus_tag="LZB08_000459"
     CDS             29234..29686
                     /locus_tag="LZB08_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7313978.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            29688..30743
                     /locus_tag="LZB08_000460"
     CDS             29688..30743
                     /locus_tag="LZB08_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBM7313979.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            30810..31118
                     /locus_tag="LZB08_000461"
     CDS             30810..31118
                     /locus_tag="LZB08_000461"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBM7313980.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            31222..32487
                     /locus_tag="LZB08_000462"
     CDS             31222..32487
                     /locus_tag="LZB08_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBM7313981.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            32575..33288
                     /locus_tag="LZB08_000463"
     CDS             32575..33288
                     /locus_tag="LZB08_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBM7313982.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSKKC"
     gene            33721..34446
                     /locus_tag="LZB08_000464"
     CDS             33721..34446
                     /locus_tag="LZB08_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP--phosphocholine cytidylyltransferase"
                     /protein_id="HBM7313983.1"
                     /translation="MKAIILAAGLGTRLKPLTSECPKPLVKVNGKPMIETQIECLLEK
                     GIKDIKVAVGYLKEKFYYLAEKYNVELIYNNKYDIYNNIYTMYLLREHLPDSYVIEGD
                     VYICNNFIDPDIKESTYFTLYKENFNNEFIFKFDENNKVYDMYVGDNKGYILCGVSYW
                     SLTDGNIIAKKLEETIENGDFESLFWDDVVMKNKDALNMYIQKIDFKDCFEIDNEKDL
                     RFSEKCIVQNNKFTMKKILQYSK"
     gene            34464..36527
                     /locus_tag="LZB08_000465"
     CDS             34464..36527
                     /locus_tag="LZB08_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBM7313984.1"
                     /translation="MGILDEIMNQEHFDIKTSLDYINEYEKLLPFDSEIHTARAVTYF
                     FGEEYKLAEEHIRKALKFRPNNYDNNFYLANILIAMGEYPDAIKSAVLSVTFLKHFAK
                     IQGYDYINDKLFYYNEQVKTLIEYCIENIKDTNVLEDLKRFFGFIELSIAKFPVCFYG
                     DKWNILVGEYLNKDLNSEYNDYICFYSDTYNNMLSDFQKKLYTVINNSEISSICPIES
                     FKSMTAKEFSLDNGKYIIPIATTEYNQVIDFKIKNTNFKSQQVDPPGFFKYYRVNNGI
                     SLNSDKDVIIGKPIKLGFKEKNKKLVFTIFIDALSQKYLLETKYKSMPYTKAFFDKGI
                     IFENCYSAGEWTYPSLTSVVTGLYTTNHHFLHSSAGYKYPDDIDLFSEIMKKNEYFTA
                     NISGTYALAPYSSGFRGFDRTVFKNSLGFSDSVIINDAIKHIESFKETNQFVFMSLSD
                     VHKILDDIENYSVNYDIVNQTSLDIETSLSKRSSFVSAWQKYDEEAIKKYDVALRELD
                     RQLKGIYDYILQNYSEEEYLISIFSDHGASFLGKDDFLLKSNITNSVLMVRGSGSASN
                     GNTEYISNMDLIPIILKLANIEHDLSKYDCVLPKTLGGKGREFTYSESIYPGQTYKAM
                     VNNDKYCYILETKGITDIDGTINFDEYSDSVFDKKEDKEVKDNDILEYYREIVYNHIK
                     RNIKY"
     gene            complement(36751..37044)
                     /locus_tag="LZB08_000466"
     CDS             complement(36751..37044)
                     /locus_tag="LZB08_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBM7313985.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(37050..37265)
                     /locus_tag="LZB08_000467"
     CDS             complement(37050..37265)
                     /locus_tag="LZB08_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313986.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            37713..38111
                     /gene="tnpA"
                     /locus_tag="LZB08_000468"
     CDS             37713..38111
                     /gene="tnpA"
                     /locus_tag="LZB08_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBM7313987.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            38108..39271
                     /locus_tag="LZB08_000469"
     CDS             38108..39271
                     /locus_tag="LZB08_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7313988.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(39655..40167)
                     /locus_tag="LZB08_000470"
     CDS             complement(39655..40167)
                     /locus_tag="LZB08_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7313989.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT        15500 a         4377 c         6964 g        13460 t
ORIGIN      
        1 tttcatactt aatttttaat atttatatta tccaaaattt tcatcttttt aacttagaat
       61 ttgagaatca ctattaggaa ataggctttg atttatgtag ggttaaaaat ttcacagaat
      121 atctaacttt atactaaatc catcaaaaaa tatacgccat ataaaagcgc ataaaaaaac
      181 acataaaaca atatttatgc gcactttaac ctcgaatggt acaaaattct ttacttttac
      241 agacccatat tatataatta agtaaataat gtatatttag gtgacatatt ggataagcta
      301 atttatttat tttattaata taataatact ataaaagtta tataaagtgg tttacaaaaa
      361 agttacagat agagctttta ttaatgtatt gtgtattagt attttatcct taggtacact
      421 tcacctacaa aatagaatca taagataatt atgggaaaat tttaggaggt ttatcgtgaa
      481 gaaaaagata ttaataccag tatttgcatc agttatggca ttatcagtaa gttcaattgt
      541 aaatgcagat gaagtaaatg attcatctca aaataaggat gataaaacaa atactgaatt
      601 aaatttaggt gaatataaag aagtaaaata taaagttgca aaaataaaag atggagtagc
      661 tataaagata agagaagaag gtcaagttca aaacatagct tattctggag atgaatttac
      721 ggttttagga actcaaggag aatgggttaa ggttaaagtt gaagatggcg aaggttggtt
      781 agctactaga tatgtagata tatctgaagg tgtaggatat actaacgctg ataaagtaaa
      841 cttaagaaaa gataagagtg aaagttcaga agtaattgaa gagctggaaa aaggaagttc
      901 tttattggtt ttggaagata atggagattg gttaaaagtt aaagatgggg aaacagaagg
      961 atatgtaaaa tcatcttata tatctgataa agctcctgta atagaagagc cacaagtaaa
     1021 cccaagtatt gaccaaaatg tagattctac aaataataat caacaaaaca atgcaaatgc
     1081 taatcaacaa aataataatt caaatgtgcc aacagctaac tctaatgcag ttcaagctgt
     1141 acttaacttg gcatattcta agcaaggttg tccatatgtt tggggagcag aaggaccaaa
     1201 cacttttgac tgttctggat ttacacaata tgtgtataga aatgcagtag gaaagaatat
     1261 accaagaaca tctaaagcac aatctaaata tggacaaact gtaagtaaag caaatttaca
     1321 accaggagac ttagtattct tcacaactaa tggttctggt tctgttagtc atgttggaat
     1381 atatgtaggt gggggtaata tgatacattc accatcaaca ggaaaaacag ttagtgtaac
     1441 tagtataaat tcatcatact atacagcaag atttgttaca gcaaaaagaa tattataatc
     1501 ggtttgtaat atgacaaatt gataaataca taaatatata taaacgctct atattatatt
     1561 taatttaatc atggaaacaa aaaatgatta tattttaaat gtactataga gcgtttttgt
     1621 atcactttaa atctaatcaa gattcaagtt aatatgactg ttaaatagga gccttttcat
     1681 atgatgatta tttgcgcaat cccttgacag gatagggcaa aggtggtaag ctactaacag
     1741 tacaaaaata cttttaattt tagttcagag aggctaaaaa aggaggacga aagatgttaa
     1801 aatcaagaaa tttaatccaa ccagaagact tttcaataga agaaatagat gaaatattag
     1861 aattagctca aaaaataatt gacaatccgt caaaatattc tcgtatatgt gaaggaaaac
     1921 tattagctac attattttat gaaccatcaa caagaacaag attaagcttt gaatctgcta
     1981 tgaacagatt aggaggacgt gtagtaggct tttcagagcc aaattcatcc tctgcatcaa
     2041 aaggtgaaac tctaggagat acaatgagga tagtatctgg ttatgttgat ataatagcta
     2101 tgagacatcc tcaatcaggt gcagcttcag aagcagctag atatacggaa gtgccattta
     2161 taaatgcagg agacggcaaa aaccagcatc caactcaaac acttacagac ttacttacta
     2221 taaaatcatt aaaaggaact ttagaaagcc atacaatagg attatgtggt gatttaaaat
     2281 atggaagaac tgtgcattct ttggttaagg ctatggcaag atataaaaat acaaagtttg
     2341 tatttatagc gcctgaagag ttaaaaatgc cagattatat aaaagaagct ataaagggac
     2401 atgcatatta tgaaactaat aatttggatg atgttatagg aagcttggat gttttatata
     2461 tgactagagt tcaacaagaa agatttgaag ataaatcaga gtatgaaaga cttaaaaact
     2521 attatatttt aaataaagct aaattagaaa aggcatctaa agatatgtta gtaatgcatc
     2581 cattaccaag agtaaatgaa atagacatag atgtagatag tgatgataga gctgtatatt
     2641 ttaaacaagc taaatatgga atgtatgtta gaatggcttt aataataaaa cttttaggta
     2701 taaatgaaga ctaatagtta tcatataata gtaaaacaaa ggagattttg ggatgtataa
     2761 aattctggaa aatatatata taggcgaaga tatgtataga atgaaagtaa aaggtaattt
     2821 tgaaggtaaa atgggacaat tctatatgtt aagagcatgg gatacttatc cagtactttc
     2881 aagacctata agtatacatg atatagatga agaagggata acttttcttt ataaagtagt
     2941 aggagaagga actcaaattt tgagtaacct aaaggtaaat gatactataa aattggaagg
     3001 cccttatggt aatggatatg ctaaggttga tggaaaggta gctctagtag gaggaggaat
     3061 cggtgtagct ccactatatt tggttgctaa aaatataaaa aattgtgatg catatcttgg
     3121 gtttagagaa gatgttatat tagaagatga atataaacaa gtatgtaata aagtctatac
     3181 aacagtagga aatacttttg taacagatat aattgatgtt gagaaatatg attatatatt
     3241 aacatgtggt ccaactccaa tgatggaaaa attagttaag atggtagaag gtactaaaac
     3301 gagaattatg gtatctcttg aaaaccatat ggcttgtgga gtaggtgcct gtttagtatg
     3361 tacgtgtaaa actaatggag gaaataaaaa gacttgtaaa gatggtccag tgttctgggg
     3421 agaggacgtg atttttaatg ggtaatttaa gtgtgaaatt tggaagtgta gaatttaaaa
     3481 atccagtaat aatggcgtct ggaacttttg gatttggaaa agaatataat gagatttatg
     3541 atatacaaaa acttggtggt ataagtagca agggtcttac tttaaataaa aaacctggta
     3601 acaatggtat gagagttcat gagacaccat ccggtatgat gaatagtgtt gggcttgaaa
     3661 accctggagt tcaaggattt atagactatg aattaccttt ttttagtaaa ctagacttag
     3721 tcagaatagc taatgttgga ggtgggacat tagaagacta cttattaggg gttcaaatgt
     3781 taaatgacaa gcctatagac attattgaat taaatatatc ttgcccaaat gtcaaagctg
     3841 gtggaatggc ttttggtata aaaaatgaag tggcaagaga agttgttcgt gaggtaagaa
     3901 acataacgaa actgccttta gtaataaaat tatcacccaa tgcagaagat attgtaggaa
     3961 tggcaaaagt ttgtgaagaa gaaggtgctg atggagtttc attagttaat acttttaaag
     4021 caatggcaat tgatataaaa aatagaagac cagtatttga aaatgtatat gctgggttat
     4081 ctggaccagc tataaaacct atagctttga gaatggtaca tgaagtgtgt aaaaatgtaa
     4141 atatacctgt aatggggatg ggcggaataa ctaaagccac agatgctata gagtttataa
     4201 tggcaggagc tacttgtatc caagttggaa ctgcaaattt tatgaatcct agaataggta
     4261 ttgaaataat agatggaatt aatgaattta tggatagaga aggaataaaa agtttagacg
     4321 aaataagagg aattatataa ggtagaataa atagtatttt aaagataaaa aatatagatt
     4381 aaataaacaa taattttata acattaacat ggaggaaaaa taatatgagt aaagtagatg
     4441 tagtggatat attaaagaaa agtgatgcat tgttggaggg acatttttta ttatcttcag
     4501 gaaaacacag taatagatac gtacaatgtg caaaagtatt aagattccca gaatatgctg
     4561 ctgaggtatt aagtacagtt gttgaacaaa taaaagactt agatatagac ttagtagtag
     4621 gaccagctat gggtggagta atagtttctt atgagttagg aagacaatta ggaaaagaag
     4681 ctgtatttac tgagagaaaa gacaatacaa tggagttaag aagaggattt gaagttaaaa
     4741 aaggagcaaa gataataatt gctgaagacg ttgtaactac tggtaaatca actatggaga
     4801 caaaaagagt attagaagcc ttaggtggag aagttgtagg tgttgcatgt atagcagata
     4861 gaactaatca tgatataggt atgcctatat atagtgctat aaaacttgat attcaagttt
     4921 atgaatctga tgagtgtcct ttatgtaagg aaggaaaatt accagttgtt aagcctggaa
     4981 gtagagagtt caaggaatta gggatgtaat aaaaacttaa ttatttatag tgttacttaa
     5041 aaaatgtaaa ttttttagag taaaaatttg taatataaca tataacttaa aaataaaaag
     5101 cagttagcct tataaatgaa ggttagctgt tttttttgtt aaaaatatat ttacaaaata
     5161 agagatagta gcagtacaat ataagtataa ggtaaaagta ttatatatta gggaggggta
     5221 agtatgaaaa atcaattaaa tgaaaatcat aaaaaggttg tattttttgg agtaggtgct
     5281 gtaggagcta cttttgcaga acaatttttt aactctaaat atgattttaa aattctttgt
     5341 gataatgaaa gaaataaaag atatttagaa gaaggattta taataaatgg aaaaaggtat
     5401 gattttgatt atgtaactaa agatgagtat aaacaagagg ctgattttat aattataggt
     5461 ctaaaatata ataatttaaa agaaaatata aaagaattag atggattagt tggaaaaaat
     5521 acagttataa tgtctctgct aaatggagtt gatagcgaag agataatagg agaaagattt
     5581 ggaattgaaa aaatggtata ttcatatgtt accaatatag atgcaaagaa aatcaataat
     5641 aatattatac acactactaa tgggataatt gtatttggta acaaagataa tagtgaagat
     5701 agaaaaacta atataataac cgaagttttt gatgatgtaa atatagaata tactttatca
     5761 aaagatattc aacgagatat gtggtggaag tacatggtta atattggtgt aaatcaaact
     5821 tcagctatac ttggtgcacc ttatggagtt tttcagagtt ctgagcattt aagagaatta
     5881 gcaaaatctg caatgaggga agttgttgct atagcacaag caaaagacat atctcttaca
     5941 gaagatgatg tggaacattc attacataga atactagaac attcaaaaga aggaagaaca
     6001 tcaatgcttc aagatgtgga agctcataga cttacagaag tagatatgtt ttctaagaat
     6061 atctgcaaac ttggaaaaaa atataacata cctactccta taaatcagac tttcttttat
     6121 atgataaaag taattgaaag tagattttaa agggtctttg taagaaatat tgataattat
     6181 tgattttaaa ttttattaat gttatatact atttaagtta ctaaatattt ttaactcttc
     6241 aatgaaaata gttgctgctt ttgataaagt tatatctttt ggaaatatat atgagaaatg
     6301 tctgttgtag ctgcttccaa gctctatgac agacagctca ttgtttagaa caggtaagct
     6361 tgtcactaga ttagagacta ttgtaattcc taaattattt ctaacagatt ctttgacagc
     6421 ataattactt ccaaaaacca tcatactttt tggaataatt tctttaacac ttaaaaacat
     6481 atctaaataa tctctggttc cagaaccatc ctccctgact atccattttt ggttttgaag
     6541 tttatcaaaa ctaaaatctt ttaataaatg actcttgtat ggaagtgcta aaaccatctt
     6601 atcttcaaaa aaatattctt gaataaatga aggggaagag caagtgcctt caataagacc
     6661 aatgtctaaa atataatcct taacatgaga agatacgatt gatgtatttt ttataaaaac
     6721 ttctacatct atgtcaggat actttttaga aaataatgct agaaaatttg gtaaaatata
     6781 ctctccaata gttaaacttg caccaatctt taagtgacca gttatagcat ctgaaacttg
     6841 gcttacatca tgataagtaa tatttaaaag atttaatatt tctttagctc ttttgtacaa
     6901 gatttgtcca ctttctgtta taaaaatggt tttctgttta atagacctgt ttataagcac
     6961 aactttaaaa taattttcta gattttttat gtgtttactt acacttggtt gtgatagatt
     7021 taaatattcc ccagctttag taaagttttt gtattcaaca acagctacaa atgtttttaa
     7081 ttcttcaaac aattttttgt ctcctttacc taatgaaatt ttacattttt tatatacttt
     7141 ctacagataa ttatactata tataataacc attaaaaaca ttaatcatta cgatattata
     7201 tatttatttt acttatggtt attacaatta tatactattg ctatgaagaa aactatatta
     7261 attataaggt aggtatatta tgttaaagaa aaaaagcagt ttgacattaa aaactataag
     7321 ggaaatatta ccaggactgt ttgtatcagt attagtaggt tatattagta tatttatttc
     7381 tattttaatt cctaaggtag gggcagcatc aatttctata tttttaggta tgtttgtagg
     7441 aaacctattt ttaaatcaaa aggtatttca aaaaggttat aaattttcag agacagattt
     7501 gttatcatat tcaatagtac tattaggagc aacactcagt gtatctacct taatagactt
     7561 aaaagtttca ggaattttat ttataatatt gcaaatgaca ataacaataa tagctgcact
     7621 ttatataggt aaaaaactag gatttgaaga aaactttagg tttctaatgg caagtggaaa
     7681 tgcagtatgt ggttcttctg caataggagc aacagttcca gtggttaatg cgacagataa
     7741 agaaaaggga attgcagtaa ctattgtaaa tgttactggt atatttctta tgtttttact
     7801 tccaataata tctcaatttt tatatagcca tgaacttgta aaaacttcag caatgatagg
     7861 tggaactttg caatcaattg gtcaggttgt agcaagtgga gcaattgttg gagaaaatgt
     7921 aaaagattta gctactattt ttaagatagt aagagtaata tttctagttg ttgttgtatt
     7981 agtatttgga catatgaaaa atcaatctaa tagggaaatt cttgaagaag aaaaggatga
     8041 gataaaaaag aaaaaagtga ctataccatg gtatgtaatt ggattcttta taacttgtgc
     8101 acttttttca atgaacatta taccacaaga agtatctgtg ttgtgcaaag aaataagtaa
     8161 caaactagaa ataatagctt tagcagcaat tggattaaag gttaatgtaa aagatttagt
     8221 gaaacaaggg aaagaagttt ctttatatgg tttatttgta ggtatagtac aagttgtatc
     8281 agctgtagtt ttaataaaaa tatttatata gcataaaaat agtatttgta atattaattt
     8341 gattaattaa aataatatga tactaataat ttataatata aataaaaaat gaatttcgat
     8401 aagtttaagt gggtataaac ttatagaaat tcatttttta tttaaggtaa aaatttaaag
     8461 taaaatatta ttttacataa taataacctt ataaattttg aaattactta tctttgtgtt
     8521 aaaataagta aaatgcacat attagagcta agtttttaag taaataaaca aaatgaaaat
     8581 ataaatatta aggagatttg gtatatgaat gtttatgaaa aaggtaatgg agttatacta
     8641 gaaggagtaa cagattttga cccagtacat atatttgagt gtggtcaatg ttttagatgg
     8701 cacaaacaag aagatggctc atacactgga gttgcaaagg ggagaatatt aaatgtcaaa
     8761 aaagagaatg ataaaatata tttaaataat actaatttaa aggagtttaa cagcatttgg
     8821 tataattact ttgatttagg aactgattat acagaaataa agaataagct aaaaaatatg
     8881 gatgaatatt taaataaggc tactgaattt ggatggggaa taagaatatt aagacaagat
     8941 ggatgggaaa tgcttatatc atttataatt tcctctaata ataggattcc tatgatacaa
     9001 agagctatag aaaatttatc aagaaagttt ggtaagtata taggtgaata tgaaggtaat
     9061 gaatactatg cattcccaac accagaagaa ctaaataagg cctcacaaga ggaaataaga
     9121 gcatgtcaaa caggttttag agataagtac ataaaaagta ctacacaggc agttatagag
     9181 aataatgatg aggtatctga atatacaaat ttaagtacag aggattgtag aaaagagctt
     9241 ttaaaattta atggagtagg accaaaagta tgtgattgta ttgcattatt tggtatgcaa
     9301 aaatatgatt cttttccagt tgatgtttgg gttaagagag ttatgcaaga attttatata
     9361 gatgaagaca tgagtttacc taaaatgaga acttatggaa tcgataaatt taaagaaatg
     9421 tctggatttg ctcaacagta cttattttac tatgcaagag aactcggtat aggaaaataa
     9481 attaaggggg agaaattatg ggagtaatag gaacaatatt tctgttctat ttaattatat
     9541 catatctagc tggagccata atttctgtga ttatattact tgaaaataga gaccctgcaa
     9601 aaacaatgtc ttggctattg atgtttatta tatttccagg tgtaggttta atgatatatg
     9661 ctatttcagg tagaaatatt agaaaaagaa aattatttaa gactcaaaaa ctagctaata
     9721 atataaaaga aaagaaacta tttgatacat tagaaaagat aacagaaata gttgaattag
     9781 aaaaagaatc tataaaacaa aataagcttt taagagatga agaggatggt agttatagaa
     9841 aaagggtaat aaatatgttg ttaaagactg gtatgtttcc ctttactaaa aataataaag
     9901 tggatgtatt tgtagatgga aatgaaaagt ttaaaagact tatagaagat ataagagaag
     9961 ctaaagacca tattcattta gagtacttta tcatcaaaga tagtgaaata ggtagggtgt
    10021 taaaagaaga acttataaaa aaggctaaag aaggcattaa gataagaatt ttatatgatg
    10081 atgtaggatg ctggagattt tggtttaata gaaaattttt ccgagaaatg agggaagtag
    10141 gtatagaaat tgcagcattt ttacctacaa aatttcctat aataggaggg aaattaaact
    10201 atagaaatca cagaaaaatt gttgttattg atggaataat tggatataca ggtggaataa
    10261 atataggaga tgaatattta ggcaaaaatg ataagtttgg atattggaga gatactcata
    10321 ttaggattaa gggtatttct gtatacatgc ttcaaatgac atttctgatt gattggtatt
    10381 atacgacaaa agaagtatta gtgactaaaa attatttccc aagtgtaggg aatgttggtg
    10441 aaagtatgat acaagttgtt gcgagtggtc cagacagtga ctgggaagat attcattatg
    10501 cttatttttc tgcaatatgt caggctagaa aaaatgttta tatagaaact ccatatttta
    10561 ttccagatga aagtctacta aaagctataa agagtgctgc tcttagtgga gttgatgtga
    10621 gaataatatt tcctaaaatt gcagaccaca agatagtaaa tatagcatct tattcatatt
    10681 ttgaagaaat attaagagca ggtggtaaag tttatttata taataaagga tttatacatt
    10741 ctaaagtcgt aataatagat gataaaattg cttctgcagg tacagctaat atggatttaa
    10801 gaagttttat gcttaacttt gaggtaaatg catttatcta tgatgaagaa gttattaggg
    10861 taatgacaga tgatttcttt gaggatttaa gttactgtga agaacttaat ttggaggtat
    10921 ttaagaatag aaatatcata caaaaaatca aagaatctgt agcaagatta ttttcaccaa
    10981 tattataaaa aattattaaa aaaggtattg aaaattatgt gtgattggta tattataata
    11041 attgttagca ctctattaga tagagtgata ataaataaaa agtatatata taaatctaca
    11101 tatataagta gtattaattt aaaaataact aaaaacatag gcgaactagt ctatacttaa
    11161 tattttagga ggcgtgaata atgaaaataa gaccattagc tgacagagta gtaattaaaa
    11221 aagtagaagc agaagaaaaa actgcaagtg gaatagtttt accaggagca gctaaagagc
    11281 aacctcaaat agctgaagtt gtagaagttg gaccaggtgg aatagttgaa gggaaagaaa
    11341 taaaaatgga attgacagtg ggagataagg ttatattcca aaaatattct ggaacagaag
    11401 ttaagataga aggacaagaa tacacaatac taagacagag tgatgtatta gctgttattg
    11461 aataaatata gaataaattt attaggaggg gtttaaaatg gctaaagaaa ttaaattttc
    11521 agaggaaaca agaagagctt tagaagctgg tgtaaataaa ttagcagata cagtaaaagt
    11581 aacattagga cctaaaggaa gaaatgttat attagataaa aaatttggtt ctccacttat
    11641 aactaatgat ggagtaacta tagcaaaaga gatagaacta gaagatagat ttgaaaatat
    11701 gggtgctcaa ttagttaaag aagtagcaac taaaactaat gatgtagctg gagatggtac
    11761 tacaactgct acagttttag cacaagctat aataagagaa ggtttaaaga atgtaacagc
    11821 aggggctaac ccaatacttt taagaaaagg aatacaaaaa gcagtaacag tagcagtaga
    11881 agaattaaaa aatcaatcaa gaatagtaga aacacaagag gctatatctc aagttgcttc
    11941 tatatctgct ggtgatgaag aagttggaaa attaatagca gaagctatgg aaatagtagg
    12001 taaagatgga gttataactg ttgaagagtc tcaaactatg aatactgaat tagatgctgt
    12061 tgaaggtatg cagtttgata gaggatttgt ttctgcatat atggttacag atgtagataa
    12121 aatggaagca gttttaaatg acccatatat attaattact gataaaaaaa tatctaacat
    12181 acaagaatta ttaccagttc ttgaacaaat agttcaacaa ggtaaaaagt tattgataat
    12241 agctgaagat gtagaaggtg aagcattatc tacattagta gtaaataaat taagaggaac
    12301 atttgatgta gtagcagtta aagctccagg ctttggagat agaagaaaag aaatgcttca
    12361 agatatagca atacttacag gtgctcaagt aatatcagaa gaattaggtt atgatttaaa
    12421 agaagctgat ttatctatgc taggtagagc ttcatctgtt aaggtaacta aagaaagtac
    12481 tacaatagta gatggttctg gagataaaaa agctatagaa gatagagtaa ctcaaattaa
    12541 acatcaagta gagcaaacta cttcagattt tgatagagag aaattaatgg aaagattagc
    12601 taaacttgct ggaggagtag ctgttgtaaa agttggagct gctacagaag tagagttaaa
    12661 agaaagaaag ttaagaatag aagatgctct taatgcaact agagcagctg tagaagaagg
    12721 aatagtagct ggtggaggaa ctgcttttgt tagtgttata ccagcaatag gaacactaat
    12781 tgagagttta gaaggagaag ttaaactagg tgctcaaata gttaaaaaag cgttagaaga
    12841 gccattaaga caaatagcta taaatgctgg tcttgaaggt gctgtaattg tgcaaaatgt
    12901 tgttaattct gaagcagaaa ctggatttga cgcattgaat gaaaaatatg taaatatgat
    12961 agaagctggt atagttgacc caactaaagt tagtagaagt gctttacaga atgctgcatc
    13021 aatagcaagc actttcttaa ctactgaagc tgctgttgct gaccttcctg aaaaagaaga
    13081 tgtaggaatg ccaggaatgg gtggcggaat gcctggtatg atgtaattta aaaaatttaa
    13141 aatataaatt ttaatataaa gaactatttc gacttagatt ttaaactcta agttgaaata
    13201 gttcttttgt atatatctaa tttttgtata tctataaatt caatacacat atttagataa
    13261 tcagtatcta gtttgtaata atattttaga gtagtattgt tattttttag gttatgattt
    13321 tagatatata ttaattaagg tataattgtt gatagagaat aaatcaaatt taaaaggatg
    13381 cgataatatg gacttaagaa aattgcttga agaagttaaa aatgaaaata ttgatataga
    13441 ttttgcgtta gaaaagttaa aagatttacc ttatgaagac cttggatatg caaatataga
    13501 ccatcataga gaattaagaa atgggtatcc ggaagttatt tattgtgaag gtaagagtga
    13561 tgagcatatt ttagggatta ttgataaaat gaatcaaaaa agttctaaca tattggggac
    13621 aaggtgcaga aaagaaactt ttctgaaaat taaagaaatc tataatcatg ctgagtacga
    13681 agaggcgtct aaaattctta agattcaaaa tcatgatata gagaatatag gaaaaggtaa
    13741 aattgtgatt gctacagggg gaacttctga tataccagta gcagacgaag cttatcatac
    13801 agctaagttt ttaggtaatg atgttgatag aatatatgat gttggagtgg caggtataca
    13861 taggctttta aataaaagac ataaaataga tagtgctaga gtaatagtag ccgttgcagg
    13921 tatggaaggt gctcttgcaa gtgtaattgg tggactggtt gatgttccag taatagctgt
    13981 tccaacttct gttggatatg gagcaaattt tggaggatta gcagcattac tagctatgtt
    14041 aaatagctgt gcatctggaa tatctgttgt aaatattgat aatggatttg gagcaggata
    14101 tttagcatca atgataaata aactgtaaat acattgatat aaattaaaca aaactatata
    14161 ataaaattta atttctatta tatttttact attaaaattg aggagacata tatgagtgaa
    14221 aaaatacttt attttgatat aataagcggt atatcagggg atatgaccct tgcaagttta
    14281 ttaaatttag gtgtgcctaa ggaaattttt ttggaggaaa ttcataaact aagaatgagc
    14341 gatgaattta atataaacat cagctcgaaa actgaaaatg gaatagtagg tacaaaagta
    14401 gaagtaatta caaaagaaaa acatacacat agaaatttag tggatatatt tgaggttata
    14461 gataaaagca ctctaaacga aaatgtaaaa tctaaagcaa aaaaaatatt tatggtaatt
    14521 ggagaagcag aagcaaaagt tcatggaact acaatagaca agatacattt tcatgaggta
    14581 ggggctatag attcaattgt agatatagta ggagcttcta tattagttga cttactttgt
    14641 gtagataaag tttgtgctag tacagtgtct gttggttcag gatttgtaaa atgtgaacat
    14701 ggaattatac ctatacctgc acctgcaact atagaaattt taaaaggtgt gccaattaaa
    14761 ttaaataatg taaatggaga atgtacaact ccaacaggag ctgcaatcat aaaagtcttg
    14821 tgtgatgagt ttgtagatga gcttgaattt tctccaaagc aaattggata tggtataggt
    14881 cataagaagt ttgaagtacc aaatatactc agaactgtat taggtgaaaa aaaaaagaag
    14941 aattaattta tgaaattagt gcaaacattg atgatatgtc ttcagaaatc tattcttatt
    15001 tgtatgaaaa aatattagat gagaaagcat tagatattta tacagagagc atttatatga
    15061 agaagaatag accagcaaca aaagtatcaa ttttatgtaa tgaatcagat ttagaaaaat
    15121 ttattaaaat attactttta gaaactagta cttttggagt gagatataat gcatataata
    15181 gagaaattct agagagaaaa tttgtaaaat tagatacaaa atatggagtg gttacagtaa
    15241 aactagggta ttataaagga aaactagtca aggctactcc agaatataat gaatgtaaaa
    15301 ctatagcaaa aaatgaagat attcctataa aaaaagtgtt tgctgatata aattttagaa
    15361 taaaagaaga atttagtgaa aacttattga cataagtaat aaattttgat aaattaatat
    15421 tcagccttat taaataaaag taaacagaaa aaaatatata aaatataaac tcactcgtat
    15481 atgctcagaa atatggtctg agagtctcta ccaagatacc gtaaatatct tgactatgag
    15541 tgaaattatt ataccaaaag acctagttaa gtaaatgtgt aggctgagtt aaactcccta
    15601 tgtgttggct ataagataaa ggtattttgg aagcctgtat agtaatttca cttttgctag
    15661 aaattatgga agttactata caggtttttt gtgtaaaaag atttaatata atttagactg
    15721 tattgtgtta aaataaatat ataaaaacta tattatacaa ggagagatgt aaatttatga
    15781 aacatgaatt agtacttgta atagattttg gaggacaata taatcagttg atagctagaa
    15841 gagttagaga aaacaacgta tattgtgaaa tacttccatg tactgcaagc atagaaagaa
    15901 taaaagaaaa aaatcctaaa gggataatat ttacaggcgg tccaaatagt gcgtatttag
    15961 aagattctcc aacaatatca aaagaaatat ttgaattagg agtaccaata cttggaatat
    16021 gctatggtat tcaaataatg tctcatgtat tgggtggagt tgtaagaaaa ggtaataaac
    16081 aagaaaaaga atatggaaaa acagctataa cttatggaaa atcatcatta tttgaaggaa
    16141 taactacaaa tagtgtatgg atgagtcaca cagatttaat agaaaaagtt ccagaaggat
    16201 ttactatagt tgcaaatact aatgattgtc cagtagcagc tatggaaaat gtagaacgaa
    16261 acctatatgg agttcaattc catccagagg tggaacactg tttagaaggg gacaagatac
    16321 ttacaaactt cttatataat atttgtaaag taaaaggtga ctggactaca gattcattca
    16381 tagaagataa aataaaagaa ttaaaagaaa aaataggtga taaaaaagct ttatgtgctt
    16441 taagtggagg agttgattct tctgttgctg cagttcttat tcataaagca attggggaca
    16501 acttaacttg tatatttgtt gaccatggtt tacttagaaa aaatgaagga aatgatgtag
    16561 aaagaatatt tagagagaaa tttgatataa atttaataag agttaatgca gaagatagat
    16621 tcttatctaa attaaaagga gtttctgaac cagaagctaa gagaaaaata ataggtgaag
    16681 aatttataag agtatttgaa gaagaatcaa ataaattagg aaaaatggat ttcttagtac
    16741 aaggaacaat ctatccagat gttatagaaa gtggacatgg aaatgctgct actataaagt
    16801 ctcatcataa tgttggtgga atcccagaag atgtagactt ccaagaaatt gtagaaccat
    16861 taagagagtt atttaaggat gaagttagaa aaataggatt agagcttggt atagaagaag
    16921 gattaatatt tagacatcct ttcccaggac caggtctagg aataagagtt ataggtgatg
    16981 taactaaaga aaaatgtgat atattaagag aagctgatgc tgtttatatg gacgagctta
    17041 gaaaagcagg tttatacaga gaaatatggc aagcttttgc aacattacca gatgttaaga
    17101 ctgttggagt tatgggtgat gaaagaacat atgcttattt agttggttta agagcagtta
    17161 cttcttctga tggaatgact tctgactggt ataagatgcc ttatgatgta ttagaaagaa
    17221 tttctaatag aataataaat gaagttgatg gagtaaatag agtagtttat gatataactt
    17281 ctaagcctcc tggaactatt gaatgggaat aaaatataga atgccaaaaa tccacatgaa
    17341 tacaggattt gaggtctctc ttacttctat gttatactgg tttgatacta tcaatatttg
    17401 ccagtatctt atggattaat aagcaaaaaa catcctgctg aatgataaat tcagcggggt
    17461 gtttttattt tgttttagaa ctcctttgct ctgaaactcg aagtatttaa cactgatgat
    17521 gttgttatct tttagttcct tttgtgtcat gctctgaaat acaaattgat acaataaaaa
    17581 agtaatttct taatcattga tggagatatg tatttatgtt actcttgatg gtaatttgag
    17641 taatattgtt catagctttt tatattcact taattagaaa accatttggt ataatcataa
    17701 ataaacttga tgatataata gaaaagattt ataattatta gaagtatata aaagtatttt
    17761 atgtctatga aatgtatgtc tataaaacat atagatgact tttgtatatt aatgtatgaa
    17821 tattgatatt atagaagaat tgagttgtca atgatactaa atcaaatatt tatagaagtt
    17881 ggagggatta tgagaaataa aataattata ggaattactt tttcacttgt attactaatg
    17941 tctggatgtg cttcaaaaca aacagcagaa ataaacgtat tagcaactac tgatttacat
    18001 ggagttatac cttatgagtt aacttcttat gtaaaagaag aaagaaaaaa agataaaaat
    18061 ctaactttag tagatgcagg agattttttt gatagtggcg aagtatctgg ttctagcatg
    18121 gatagttatt ttagtgctag gtataaggat attgataata acactgagcg ttacattgag
    18181 acacctattt caaaagatat gaaagaggta ggttatgatg ttgcagttct tgggaatcat
    18241 gaatttatat ccaacaataa attttatcta gataatatga tatctgattt tgagaaacaa
    18301 aaaataaata ttttatcggc aaatacgtat aaaaaaaatg gagatagtta tacaaaacca
    18361 tacataataa aaaatattaa tacacctgaa ggaaacgtaa aactaggaat tttagggctt
    18421 actataaaag aagttggtga gaggaaacag tggaaagatg gaaaacttgt tgactctaag
    18481 tctttagaac taaaagacca agagggttac aatggtgaat tgtatatgaa tgatttagta
    18541 gaagatgcta aaaagtgggt tggtataatg gaaaaagaga agcctgacat tatagttgct
    18601 gtggcacata caggtgagaa acctaagaaa cctaaaaacc caggaaatag aatacaagac
    18661 ttagctcaac aagttgatgg aattgatgct atagttgcag gtcataatca tgttcaaatt
    18721 gagcaacatg attataaaaa taaatcagga gaaaatgtta tagttactga gccaggaaag
    18781 catggggaat gtatttctaa gattaatttt aaattagaaa aaaacaatga taaatggaat
    18841 atagttgaca aatctagtaa attaattaaa tttgaaaaaa atcaagaaag tgataaattt
    18901 ggagaattta tttcttcaat taataaagta agtaaagata agaaagaaat aagattatca
    18961 gaagtaaaga ctcctgaatg ggataaaata tatgttttta aatctggaac atctgcagat
    19021 gatatatata aaacaatagg atataaatgg agaaatatat catctccaaa gggtgatgac
    19081 atggtgcaga ttgttgtaat gaataataat aagcctgtta gctatgtata tggtgaagct
    19141 gaagttatgc caattaatat aaaatgtgat gaatcagagt ataaagataa tgttgtaact
    19201 atttatccta ataaaaatga taaatttaaa attgaaaaag gtaaacaaga atatagtatt
    19261 aatttaaaac atatacaata gaaactttta tcttatttaa gtttagattg tacttaaaat
    19321 aaagtgtgta ttattttaaa aattgatgat aattaaaatc acaacattaa ggaatagtac
    19381 acacttttta tgctaaaaaa tagaatgtta aaaaattaaa cctttaaatt aaagtacatt
    19441 gcttatatta tccaatatta tcttcaaagg ttgtgtaata tttttatcct tatgtaatat
    19501 tgctaacatt ttattttcca tatctatccc atctaattca atcaatcgca attttttatg
    19561 aaagagtgaa tctaataata aattttctgg caaaatggtt atacctaacc ctgcttctgc
    19621 agctttaata agtgcaaaag aattaacact ttcccaaaca ggataagctt tttggtttga
    19681 aagagataat acactgtcca aagtattgcg aatggcactt ccttgctcac gtaacaataa
    19741 cggaaacgtg cataactgtc gtagagataa ggcttgttta gtattggtgg tggttatgca
    19801 atcatacctt taatccaaga gaaagtagta aatcaaactg gatggataag tgagaaaata
    19861 tttacagata ttataactat ttcacaaatg acaccagggc cactggctgt taatacttct
    19921 acttttgttg gacttcaaat tgtagggttt tggggagcag ttgcagccac tttaggttgt
    19981 gtattatgtg gtgtagtaat atctctattt ttatataatt tttttcaaaa gcatcagaac
    20041 tctacttata tatttgaaat attaaatggt ctaaaatcag cttcattagg tttaattatc
    20101 tcagctgcgg ctacaattat aattcttgca ttatttggga cgaataaagt gtacttcaat
    20161 tctatatttg agatgctaaa ctggactgca ctaataatct tttttgttac actactgata
    20221 gtacgtaagt ggaaaattaa tcctattgtg attataataa taagtggtat actgggaata
    20281 tttttttatt tataagtttt tgctaagtaa aggatatcta tttttttata tcttaaatcc
    20341 aaatagccaa taaagtaagg ctatttggat ttttaataaa ttctgaaaag ataatcagaa
    20401 acccatataa catttaaatt atatcactgg ttttctacaa accaatcata agcttcttgg
    20461 caaaatttct cactatctat atagtaacta ttatgaacat tattaatttc aacaagtttc
    20521 tttttatatt ttagtgaatt ataatagcta tcaaaatatt gggttttaaa atatctatca
    20581 tgaagtgttt taaagacaag agtaggtatg ttgactccat cttttatctt actatcttct
    20641 gcaataaagc attctaacat tgtattaatt atctttgtac tatttttcca aatatgagga
    20701 tttttctttt caaatatttt ataaaattca ttatcataga cttcattata acctttatat
    20761 ttaggtggtt ttactgttat gtgaaaatta ggaagaaact taaagaaaaa atataataat
    20821 ttttatgttc tgttttatat attattcata ttttatcaca gataatatac taaagttaat
    20881 ttttttattg tacagctatt ataataattc ttctataata taagacattc tataatttta
    20941 tatttaagtt catgtgtatt taattgtttt tatataaaat gtccagtaat actatttgga
    21001 tttattttta tttcatcagg agttccagaa gcaacaattt cccctccatt tattccactg
    21061 tctggaccta tatcgataat ccaatcagaa gcacgtataa cctctgtatt gtgttcaata
    21121 attaaaagtg aatgctgttc atttacaagt tttctaaaag taattaacaa attttctata
    21181 tctttaaaat gaagtccagt agtaggctca tccaatatgt acagcattcg ttttttagtt
    21241 tttgtcccta attcttttgc taattttatc ctttgagatt ctcctccact taatcctaat
    21301 gtattttgtc caagctttag atatccaagt cccacttcat ctaataagga gagttttcta
    21361 tatatatcag agcaatcgct aaaaaatgtt tttgcttctg ctacttccat gtcaagtata
    21421 tctgcaattg tctttccctt gtattgtata gacagtattt ctttttgata tctcttacct
    21481 tgacattcac tacaagttac ccattggtct gccatatatt gcagaataat ttttgtttta
    21541 cctaatcctt gacagtttgg gcattgcccc tttttgctat taaagctaaa atagctttca
    21601 tccaataatt tttgttttgc ttgtggagtt tcagcaaata gagaacgaat ttcatcaaaa
    21661 atattgatat atgtagcagg tgtagaacgg ctagattttc caataggtgt ttggtccatt
    21721 aagataaaat catcaatagc atcaaatcca gtaaattcag tataattttt atctgggata
    21781 gatttttgtt tcaatttttc ttctagagca ggaagtaaac tgtgaaatac aagagagctc
    21841 ttaccagaac cactaacacc tgtaatagag cacatacagt ttaagggaat ttctacatca
    21901 atatttttta agttatttgc atggcatcct tttaagctta accatttact tggtatagtt
    21961 ttattttgtg aacctgtttt attgtatgta cttaaatatt tacctgttat agagtttgga
    22021 ttttttataa tttcttgggt tgtaccttct gcaataatga accccccttt tgtaccagca
    22081 cttgggccta tatcaataat ataatcagca gacagtattg tatctttatc gtgttccaca
    22141 actataacag tattaccctt atctcttagc tcttctatca ttttgatgat taaattatgg
    22201 tctcttgggt gtaaaccaat agatggttca tctaaaatgt aggttaagcc tactaattca
    22261 cttcctagct gacttgataa tcgtactctt tgcagttctc ctccagataa agttggagct
    22321 gttctatctg ttgatatata gctaagacct acctttaata gataactaac tcgaattttt
    22381 aattctgtta agatatcatt taccaaagag agctcatttt tttgaagttg atttggtaat
    22441 tcacacaacc aattccataa ctgttctatc gtcatttttg ttagttcagg gaagcgataa
    22501 cctttgattg tagtgaatct agcctcaatg cacagcaatt cgccaccaca tgtattgcaa
    22561 ggaattttgt tcatatattg atgcaaggta ttattttcag aactagattc tctaaaaagt
    22621 ctttgaatgt ggttgattgc tcctgaagca ggtcttctaa tctttgattc tcgtcccttt
    22681 gattcaaagc taaattcata tatcttatcg tctgtaccat ataagattgc atctataaaa
    22741 tctcttggta attcattcca aggtatatct aggtctatat ttttatcttt tgctattgca
    22801 tataattcac ctaccatcca attaccattt ggctttttcc ccctcaattt tccaaaatag
    22861 taagtagctc catctaatat tgaaatagtt gggtctttta caattaattg aggattaact
    22921 tgataggtaa aacctaatcc attacaatcg tgacaagctc cagtatggga gttagcattg
    22981 aaggttgaag tatttatctt agaaaaaata gtttgacaat gcggacaata attttttgta
    23041 ttattctttt tttctaatgg aattttacaa tatggacagt aactttttcc gattgataca
    23101 aataacattc ttaaataatc ccaaatgctt gtaagagtac caacagtaga gcgaggattt
    23161 tggcttcctt ttttctgttc gatagctata gtaggggtta atccctgaag taaatcaaat
    23221 tccggttttt gaagctgttc tgaaagtttt gctccattac ttagattttc aaaataacgt
    23281 ctttttcctt cttcataaat cgtatcgaaa gctaaagaag attttccact accacttaca
    23341 cctgtaataa cagttatttg attttttgga attgtaacct caacatcctt taaattgttt
    23401 tgccttcctc cttttaaata gatagagctt ttattatgac aaagtgaatt tttaattgat
    23461 ttatttattg attttgtaaa tttgaatttc ttttctagtt caagaggatt gcctaattct
    23521 tttatttccg aaatgcctaa tatttttaac tgagaaaaga ttgatttcaa ataatcttca
    23581 ggcaattttt ctattaacaa attaaggtct tctattttta tatttccgct tgataatatt
    23641 tctgccattt catctaataa atttttttct gctcttgtta attgattgta ttttttggaa
    23701 cttaattctg tccataaatc ccatggaagc aattctaaac caatcatatt attaaagtca
    23761 tgtagaagat taccttttac agaatgccaa cctttccaat ttttattgta tccaaagtca
    23821 tctggatgag catgtccttg cctacatctt atccaggctt ctgaaccagt taaaaaacca
    23881 tctgtttttc cgacatcatg agtgtcaaaa ttaatttgaa aataattttt ttgtatgtca
    23941 tctatttgtg catctattct tatccatctc tttgttagag tatcatagta ttctacaagc
    24001 cagtggtctt cataagtaag ctcactttca aaataattag caaaacctgc tctcattcta
    24061 gcctcaaaac ctcgatatct taggagcgat acaagaagta aagaataatc tctgcacatt
    24121 ccaacaagcc tttgttttgg gtttcttgga atagttagtg ggttagaatt tatttcaaat
    24181 attcttttta acatttgagg aattgtacga agcaattcct catcagattg ttgcttatta
    24241 aaggatatac catataattt tccttggtct ccatgaatta ctaggctttg aaccactttt
    24301 aataactcaa ttggagtctt tggtaaatct tgatatttat tttcaaattt tttaggattt
    24361 gtgatgatgc tttgattttt atagttattt attttcattt atatttcacc tctctaaatg
    24421 gatgatatac cctgacatgg tgtcatatgc aagggcaatt tagatttttt gaccaaaatt
    24481 taagaataaa taaaaaatga tacagtgata aattgtaata caaatatagt ttcaaattgc
    24541 atataatata attatttaat aaaaccatta tctatggaat gttaatgaaa tgttaagatg
    24601 gcattcttga atattaagaa atagaatact ataatatagt gtgaaggatt tttagtaaaa
    24661 aaatttgtaa gtgttaatat acattaaaaa ataggtttat aatttggagg aaaaaaatga
    24721 aattatcaag gtataagaaa aatagtttag ttctgtctat aattttatgt tcacttatta
    24781 tttcaatcct tgtaattact gtttttaata ccgtaaaact acacaatata tctaataaac
    24841 aatctaaaaa ctatttaaat gatgtatcta cacagattgt aatgaatgtt gattctaaaa
    24901 tcaaatttat tttatccgac ttaagaataa tggcagattt tattaagcaa tatgaaggtg
    24961 atagtcgata tgagtattta tctaaaagaa agactagtta taagtattat gatgttggaa
    25021 taatagattt agaaggtaaa gctgagtttt taagtggaaa aaagtttgac ttaaagaata
    25081 ctgcttccta taaaaaagga attgaaggta aagaatactc agaagtaata gagtcaatgg
    25141 gttttgttct ttatagtgta cctatattta atgataatca tgaagtatct agtattttaa
    25201 taggtgtatc caataaagaa agtatgaatg agattcttaa tatagataac tttaatggag
    25261 aaggaactat tgaaattatt aatagtaagg gaaaacctct tttcatagga aaaaatactg
    25321 aattaataaa agaccttagc cataaatata aggattctac agatgaacct tgggcacaaa
    25381 agatgcttaa tgattttaaa aatgataaat cagggaatat tacaataacc tcatcaaaag
    25441 gtgtacaatg cttattgact tatcatccaa ttacaagagg tttaaatgat tggcattttc
    25501 ttctcatagt acctgaagat gctgtacttg gtgaacttaa taagttaaat tcttttacaa
    25561 taacaatgac atttataatt acatgtatta ttggaattat aacattaatt ttatatatta
    25621 taagaagaaa atatgtaaat caaatagaaa atattgcata tttagatagt ataacaaatg
    25681 gcataaattc aactaagttt agtatgctag tcaaaccttt aatttcagaa tcacctgata
    25741 gtacttatat gatgatagca atgaatataa aagattttaa gctaataaat gattgtttcg
    25801 gtagtgaaaa gggaaatatg acactaaaac atctttacaa tattttgtat aaaaacattg
    25861 atgaaaatga agaatttgtg tgccgtcatg atgcagacct tttctatttg cttattaaga
    25921 atagaccagt gttagaagcc ctggagttat tatataaaat agaagctgac acaaattatt
    25981 ttaataaaaa tagggaaaat ccatactttt taagactatc agtaggtatt tacacaattg
    26041 aaaatcataa tgaagattta attacaatac aagaccatgc aaatacagca agaaaaagct
    26101 ttaataaaac tcatcaaagt gatttttctt tttatagtga tatagaaaga aaacgattaa
    26161 tatctgaaaa ggaaatttca aatttaatgg aaaaagccct tgaaaataaa gaatttttta
    26221 tgtgcttaca acccaaaatt gatataaaaa gtggaaaaat tagtggagca gagtctcttg
    26281 tgcgttggaa aaattcagaa aaaggcataa tttatccaag tgattttatt cctcttttcg
    26341 aaaaaagtgg atttatatgt aagttagatt tatatatact agaagaaaca tgcaaattga
    26401 tttctaagtg gataaaagaa ggtaaagaag tgttgagaat ttctgttaat gtatcacgtc
    26461 aacatctgaa tgataaattg tttttagaaa aatataagca tatatgcaat aaatacaatg
    26521 taccaacatg tctgatagat ttagaactta ccgaatctat tttcttagaa aatccagaag
    26581 ctatagatat aatagaggat attcattcta atggatttaa gtgttctata gacgattttg
    26641 gatttgggta ctcatcatta ggaatattaa aggattttaa agttgatata attaagttag
    26701 accgttcatt ctttgttagc aaaaataata tagaccgtgg taaggtagtt attaagtcta
    26761 ttattgaact tagcaaaagg ctaggtatga aagtaactgc tgaaggaatt gaagaattag
    26821 aacaagttga gtttctaaaa aatgtgggat gtgattatat tcaaggttat gttttttcaa
    26881 aaccattatt aatttctgac tttgaaaatt ttgcatatga aaataataaa attaaaaata
    26941 tccattatta aatgtacaag ataaaaaatt taggcaagtt atataattaa aaatataact
    27001 tgtctttttt atttatatat ttaataaaaa tatatttgca gattttgtaa aaataatttt
    27061 caaatgttgt aagttgtggc taaagcttta ttgtattatt ctgaatatag aaagatagtt
    27121 actgtcttag ataaaaactg aaacatacat aataatataa aatttgctat attaataact
    27181 ctaattattt tggaaggagg gaaaatatga taagtgggag aatgttactc atcatgaatt
    27241 ttctaaaagg aaaaaataag acaagttaca aggatattag caaacaactt agtatagaag
    27301 aaagaaaagt tagatatgat ataaataatt taaatattgt tttgcgttca ttaaacaagt
    27361 cagtaataca aaagatgaac aaaggtgtgt taattattcc tgatgacttc caagtagtat
    27421 gtataaataa taacgaatat gtattttcct taactgagcg agtatctatt atgaaaatta
    27481 tatcaatgtt taaaatagat aatctaaact tagaaaaatt aagtaaagaa tttcaggttt
    27541 ctagaagttc aattaaaaat gaccttaatg ttttgtcaag tgaattagaa agaaaccaaa
    27601 taactattac ttatgataaa tcttttaaaa taagtggaga agaagacatt atatataagc
    27661 aaagattgaa catattaaaa tcgtattctt atctttttgg taaggaaaaa tctgatttaa
    27721 gtgtatttga gaagtattta gtgaatgtaa ttgaaaaaat attcaaaggg atttatttga
    27781 ttgatatata taattggtct atgaatttat tattgcaaat gggatggact ttaaatgatg
    27841 aatcttttac atggtatgtg tcaaacattt tcctttttac atggtatata cattcagatg
    27901 aaaaacatcc tttagaatct gcttcattaa gtgagccgtg ttttgaaaat aagtttgacc
    27961 aagaatttga aacaattatt caaaaaaagt tgactaagga acaaattttt ctgttaaaga
    28021 gttttgtatt ttttacaaat aaatatgcaa gtttaaacga ggatatggat ttgattagta
    28081 cagaaattat agttcaagac ctaatttaca atatgtcaga aatattagag attagcttta
    28141 aagaagatat gattctttat aaaggattac tcaatcatat tgccccattg atagagagaa
    28201 tcaagcagaa tatacagatt tatgaggaat tacttcatgt aataccagat aaatatcaat
    28261 atgtacttga agctactaag gtttctattc aaaaaaacag agttttaagt caaataaaga
    28321 atgaaaatga aacaatactt ttggcaatac atttcttagg aagtatacat agaaatgaga
    28381 ggaataacta caaaaatata ttgttagttt gtggatttgg atatggtgta attgctatgt
    28441 taaaggatag tttagtaaat gattaccaga taaatatagt agagagtatt ccatcatata
    28501 agttacaaga ttataaagaa tgggaaagta ttgacttcat tataacaaca tccaagatag
    28561 taatagactt gcctaaaaca attattgaga taaatccatt tttacaagaa gaggattatt
    28621 tgaagtttga agaaaaaggg ataaaaagaa agaacgtctt aactaattat atgtcaataa
    28681 aaaaaaggtt ggacttttta gaagaagatg ccaaaaaaag agtattaagt attatatgtc
    28741 aagaacttgg atacagtgat gagagaatat tatttagaca acttaattta agtgatttaa
    28801 tagggattga taccataagg ataattgatg aagatatgaa atgggaagat gctgtaaaaa
    28861 ttagttcaaa catacttgct aattgtgatt ttgtatcagc acaatatgct gagaatatta
    28921 tagatatttt aaaaaatgtg ggtttttatg ctgtaaaaga taacgaattt gctttactac
    28981 atggcaatga tagttcatta gtcaaagtaa gttcgataag tttattaata tgtaaagaag
    29041 cagttagttt tggagataaa aaggttaaaa tcatattttg cctagcaagc agagataaaa
    29101 aagagcaaat tcctgcaata attaatctaa ctaaaatggt ttataagact aattttattg
    29161 ctatgttaga aagtgcaaag acaaaagaag aagcagaagt tttaattcat aaatatgaga
    29221 aggaggtcac tgtatgaata aattagttaa tgtaaaatgt tcatttaaaa tacatgttaa
    29281 ttcaaaagaa gaagccatta tatcgttagt tgatgtagtg tcaaaagaag gatatttaat
    29341 agataaaaat caatttttaa aagatgtttt aaagagagaa gaaacattgt cgacttatat
    29401 tggacatggt ataggtcttc ctcatagtca aagtgtaggt gttaaaaact cttgtattac
    29461 aataggaaag cttgatactc ctattgaatg gacagaagaa ggagaaaaag tggatttaat
    29521 atttttgata tcagtaacaa aagacaatga aaataatctt catttaaaga ttttatcaaa
    29581 attagcacgt ttattgatgc atgaaagttt tagaaatcaa attagatgtt cagatgaaca
    29641 aactgtttat aatcttataa aagaaaaaat agaggaggaa gattaaaatg agaattttaa
    29701 agtttttgca aaaacatctt atgacagcaa catcttatat gattccattt gtggttgcag
    29761 gaggaatatt atttgcttta tcagtaactt taagtggaca agctgctgtt cctgaaacag
    29821 gctggttggc taaactgaat caaattggag ctgctggctt ggctttattc attcctatat
    29881 taggtggata tatagcattt agtatggcag ataaaccagg attagctcca ggtatgatag
    29941 gtgcatattt agcaaaagaa gtaaatgcag gttttatagg tggtatcata gcaggattta
    30001 tagcaggatt tgttgtttta caattaaaga aaattaaatt agctcctaca atgagaactt
    30061 taggttctat atttatttat cctttagttg gtacattgat tacaggtggt ataattgtat
    30121 ttttaattgg agaacctata gcttctttta tgacatggat gacaaactgg ttaaatggaa
    30181 tgtctggtgt ttctaaaatt cctttaggtg gaatcttagg aggaatgata gcctcagata
    30241 tgggtggccc tataaataag gttgctgcta cttttgccca aactcaagtt gacacacttc
    30301 catatttaat gggtggagtt ggcgttgcaa tttgtatacc tccaattggt ttaggattag
    30361 ctacattatt gtttccaaaa aaatttagca aggaagaaag agattcaggt aaagcttcac
    30421 tattaatggg atgtgttgga attacagaag gagctattcc atttgcaaca tcagatccag
    30481 ttagagtaat accttgtatt atggctgggt cgattgtagg aaatattatg gcttttttat
    30541 taggatgttt aaatcatgca ccctggggag gtctgatagt gttacctgta gttgacaata
    30601 ggttggggta tattgcatca gtattaactg gagcagtagt tgttgcggta ttaatgaaat
    30661 tagtaaaaaa agacgtgaaa gaagatgaag aaattgaaga agatttagat gaatcaattg
    30721 aattaatatt tgaagaatta taaatataaa tggaaggaat ttgtataaaa aagtaaatat
    30781 aaaactataa atttaaagga gataagaata tgaaaattgt agcagtaaca tcttgtccta
    30841 gtggagtagc acatacttac atgtcggctg aatcacttga attatcagcc aaaaaatttg
    30901 gtatagaaat aaaggttgag actcaaggaa gctctggaat agataatgaa ttgtcactta
    30961 aagatattga tgaagcaact tgtgtaatat tgactaatga tgttgagatt agaaatatgg
    31021 aacgttttaa aggaaagaag gtagtaagaa tgagtgtatc tgagataatt aagaaatctg
    31081 atgcattaat taagaaaata aaagatactt ttcaataaaa attgatttat tttacatata
    31141 actcaaagat gtttttaaag aaatttttat taaaagcatc tttgaaaata tcttaaaaat
    31201 agaataaaag gagatatagt catgaaaaag ttaccaataa agactgtcgt gcaaagatta
    31261 ctaaacttac aagaagaggg gaaaagtgca actcttttag gaattggtcc tatgtcacca
    31321 aacttgttac aagcaagttt tgaactagca aaagatgatg attttccttt aatgtttatc
    31381 gcaagtagaa atcaagtaga tgcagatgaa ctaggtggcg gttatgtaaa tggttggaat
    31441 caggaaactt ttacaaaaga tattaagaag gttgccgaca aagttggatt tgatggttta
    31501 tattatttat gtagagacca tggtggacca tggcaaagag ataaagagag aaatgaccat
    31561 ttgcctgttg atgaagcaat ggagttagga aaaaaatctt atttagcgga cattgaagca
    31621 ggttttgacc tattgatgat tgaccctaca aaagacccat ttgaaattgg aaaagtaatt
    31681 ccactagatg tagtactaga aagaactgta gaattaattg agtattgtga aaacgagaga
    31741 aaaagactta atctaccaga cataggttat gaggttggta cagaggaaac caatggaggt
    31801 ctgacttcaa ctgagacgta tgaaacattt ataactagac taaaggttga actcgataat
    31861 agaggtttgc caatgccaac atttatagtt ggtcaaactg gtactttaac tagaaagaca
    31921 gaacaagtag gaacttttaa ttttagaaat gcttatgatt tagctcaaat ggctaagaaa
    31981 tatggagttg gtttaaaaga gcataatggc gattacttag atgatgtaac tttattagaa
    32041 catataccat ctcaaattat tgctacaaat gtagctcctc aatatggtac tgaagaaact
    32101 agggcatatt taaaattagc agaagttgag tgtaaattag aaaaagaggg cttagttgag
    32161 aaaacttcta atatcagaca tgttcttctt gttaatgcaa ttgaatgtgg acgttggaga
    32221 aaatgggtag taggagaaca aaaaaatcta actactgaag agatatttaa ggatgaagtt
    32281 ttatctaatg aaatactaga tattgcagga cattatacat ttaataatga tgatgttaaa
    32341 aaagaaatag aagtgcttta tgataattta agtaagaata atattgatgg tcaaagattt
    32401 gttgtagacc atattaaacg tcctttgcgt gattatgcag aatgctataa cttaaaaggt
    32461 gttactacga ggattttaaa taaataatag gacttatagt ggtgagtatt gttatactag
    32521 gtatttatag ttataattat acttaccact attaaattta ggaggactaa tattttgatt
    32581 aatttaattt ttgatgtaga tgatacttta tataatcaat taacgccttt ttacactgca
    32641 tataataaag tgttttcttc aataaaggat atttctattg aagatttata tatgagtagt
    32701 agaaagtata gtgatgaggt atttcatatg actgaaaatg gagaaatgcc aatcaaagaa
    32761 atgcatattt ataggataat gaaagctttt gaggagttgg gaaattctat tacagaaaaa
    32821 gatgcacaaa gttttcaaga tgagtatata tatcaacaat cacaaattac actaatacca
    32881 gaagttgaga ggattttaaa tttttctaaa gaaagaaata ttaatctagg aattataact
    32941 aatggcccat caaaccatca aagaatgaag ttaaagcagt taaatattga aaactgggtt
    33001 gataaaagta atatttttat ttctagtgag gttggatttt ctaagccaga tactaatatt
    33061 tttagagtag ctgagaatgt tatgaattta gatagagaaa atacatatta tgtgggtgat
    33121 tcttatagaa atgatgtatt aggtgctaaa aaagctggtt ggaagagtat atggttgaac
    33181 catagaggac atgaggtaga agagttgttt tacaaacctg attttgtaat tttggaacat
    33241 aaggatttga tatctttatt tataaaaata tgtagtaaaa aatgttaata atagaattgt
    33301 ataaaacaat taaaatatgt gtaaaatcat aaatttaagt tatttacaat atgttttata
    33361 ttaagaattg attatttgga gaaatacttt aatatcaaaa aagatattaa agtatttttt
    33421 gatttaaaag attttttata ttaataatat aagttttaaa aagtattgtc cgataaataa
    33481 aaggataggt aattattagt ttgaattata taaagtttga tgaattatat taaagatgat
    33541 aagttaaaat tcagattaaa tttagacctt tagaataaat aaaaataaac attaggttta
    33601 aaatgaaatt taatatatac taataaaaaa tttctatcgg aatatttgga gattttataa
    33661 gtgatactaa cttaaaacct ctaaaacatt acaatattca attggaaggt aggattaaat
    33721 atgaaagcaa taattttagc agctgggctg gggacacgat taaaaccatt aacttcagag
    33781 tgcccaaaac cattagttaa agttaatggg aagccaatga tagaaaccca aatagaatgt
    33841 ctattagaaa aggggataaa agatataaaa gttgcagtag gttatttaaa agaaaaattt
    33901 tattatttag cggaaaaata taatgtggaa ttaatatata acaataaata tgatatatac
    33961 aataatattt atactatgta tttattaaga gaacatttac ctgatagcta tgttatagag
    34021 ggagatgtat atatatgtaa taactttata gatccagata taaaggaatc cacttatttt
    34081 actctttata aagaaaattt taacaatgag tttatattta aatttgatga aaataataag
    34141 gtttatgata tgtatgttgg tgataataag ggatatatat tatgtggtgt atcctattgg
    34201 tcattaacag atggaaatat tatagcaaaa aaattagagg aaacaataga aaatggagac
    34261 tttgaaagtc tattttggga tgatgttgta atgaaaaata aagatgcttt aaatatgtac
    34321 attcagaaaa tagattttaa agattgtttt gaaatagata atgaaaaaga tttgagattt
    34381 tcggaaaaat gtatagtgca aaataataaa tttacaatga agaaaatttt acaatactct
    34441 aagtaggtta ggaagggatt tgtatgggaa ttttagatga aataatgaac caagagcatt
    34501 ttgatataaa aacatcacta gattatatta atgagtatga gaagctacta ccatttgata
    34561 gtgagattca cactgcacgt gcagttacat atttttttgg agaggaatat aaacttgcag
    34621 aagaacatat tagaaaagca ttgaaattta gaccaaataa ttatgataat aatttttatt
    34681 tagcaaatat attgattgct atgggtgaat atcctgatgc cattaaatca gctgtgttaa
    34741 gtgtgacatt cttaaaacat tttgctaaga ttcagggata tgattatata aatgacaagc
    34801 tattttatta taatgaacaa gtaaaaactc ttattgagta ctgcattgaa aatataaaag
    34861 atacaaatgt acttgaagat ttaaaaagat tttttggttt tattgaacta agtattgcta
    34921 aatttcctgt ctgcttttat ggggataaat ggaatatttt agtgggagaa tatttaaata
    34981 aggacttaaa tagtgagtat aatgattata tatgttttta ttctgacact tataataata
    35041 tgttatcaga ttttcaaaaa aaattatata ctgttattaa caattctgag atatcttcaa
    35101 tatgtccaat agaaagtttt aaatctatga ctgcaaaaga atttagttta gataatggga
    35161 agtatattat tccaattgct acaactgaat ataaccaagt tattgacttt aaaattaaaa
    35221 atactaattt taaatcacaa caagtagatc cacctggttt ttttaagtat tatagagtaa
    35281 ataatggcat tagtttaaat tcagataaag atgttataat tggtaagcca ataaagctag
    35341 ggtttaaaga aaaaaataaa aagttagttt tcactatatt tatagatgct ttatctcaaa
    35401 aatatttatt ggaaaccaaa tataaatcaa tgccttacac aaaagctttt tttgacaaag
    35461 gaattatatt tgaaaattgt tattcagcag gagagtggac atatccatct ttgacaagtg
    35521 tagttacagg gttatataca actaatcatc attttttaca ttctagtgca ggatataaat
    35581 accctgatga tatagatttg ttcagtgaga ttatgaaaaa aaatgaatat ttcactgcaa
    35641 atataagtgg tacatatgca cttgcaccat atagcagtgg ttttagagga tttgatagaa
    35701 ctgtttttaa gaatagttta ggattttctg attcagtaat aattaatgat gcgattaagc
    35761 atattgaatc atttaaagaa accaatcaat ttgtgtttat gtctttgagt gatgttcata
    35821 aaatattgga tgatattgaa aattatagtg ttaattatga tattgttaat caaacatctt
    35881 tagatataga gacatcttta tcaaaaagga gttcatttgt aagtgcatgg caaaaatatg
    35941 atgaagaggc tattaaaaaa tatgatgtgg cgcttaggga attagaccgt caattgaaag
    36001 gaatttatga ttatatatta caaaattact ctgaagaaga gtatttaatt agtatttttt
    36061 cagatcatgg agcttcattt ttaggtaaag atgatttttt attaaaatca aatataacta
    36121 atagtgtatt aatggttaga gggtctggaa gtgcctctaa tggtaataca gagtatataa
    36181 gtaatatgga tttaatccct ataattttaa aattagcaaa tatagaacat gatttatcta
    36241 agtatgattg tgtacttcca aaaacactag gaggtaaggg gcgtgagttt acctatagcg
    36301 aatcaatata tcctggtcag acttataaag ctatggtcaa taatgataaa tattgttata
    36361 tattagaaac aaaaggcatt actgatatag atggaaccat taattttgat gaatacagtg
    36421 actctgtttt tgataaaaaa gaagataaag aagttaaaga taatgatatt ttagaatatt
    36481 atagagagat agtatataat catataaaaa gaaatattaa atactaatat taaaaattat
    36541 ttacatatta aatagatata atttattgta ttttaataaa tattctacct ttaaaataca
    36601 actatctttc aaaatataac tattgaatta gagggaataa aaatttttac acaaaaaatg
    36661 agatactcaa atttttattt tgagatatct cttttttgtt atattattat atagttaaaa
    36721 taaaataatt atgaatatat tttatttaga ttaatcaata acattttggg attctttata
    36781 actacatgtt aactgttgaa taggtttatc atatggttta acccatcctt tattaatact
    36841 taattctgta agtgctgtgt gaccttctac catttgagtt aatgaagctg aatatatagc
    36901 tcttaattca ggtgtactac ttgtcaatgc agaattaaga tacatattgg cagatgcttt
    36961 agcagatgaa agcatactta acattattac tttatcgtct atataagtat tgttcttaat
    37021 tagattttct atcatatttc tcatgttatc tatacctcct cagaaattag tatttcattt
    37081 tcaactataa attgttgtat agctttaatt cttccttctg ttgctaatat actagattct
    37141 gattgtcttt ttaggtcttc atctgatatt aacatattta ttgctctttg cataagtaat
    37201 ccgtcagact ccatttttat aattgctgct aaagatattt tttcagtttc tgaaagtatt
    37261 ctcatttaat catctcctat attgtaattt atgctttaat agtatgtctt tcttttcaag
    37321 gaaataacca ttacactttt ttacagtatt ttatattctc tttatatcaa aatttagtcg
    37381 acctaatata ttaatataat tttgtaggat atttatcatt aaatatctat aaaataaatt
    37441 atttgaatat tcataaagca aaatttattt gtatgtactt cttcaagagt caatatgagt
    37501 tttttcaaaa aattaatttt tatgataatc attaagggtt atatttttct taaagttgtg
    37561 attatcatta ttaatttaca ataaaattgc cgaaaagccc tctcctttag gtgtagggat
    37621 gaaaggtaag gtcttaatat aaaaaaagtt agaataatgc ttgtaatcta ataccgctac
    37681 tgatacccta tatttaggag gtgttagttt tcatgtataa atctaacaaa aatatagttt
    37741 atgattgcaa atatcatatt gtattttgcc ctaagtatcg taaacctgtt ttagttggcg
    37801 aagttgaaaa gatgttaaaa gaaatactac catataaagc agatgaactt ggagcagaaa
    37861 taatagaaat ggaaactgat aaagaacatg tgcacttatt aatatcttgt gaccctcaat
    37921 atggaattca taaagtagta aaaggtttaa aaggctttag ttcgagagta ttaagagaga
    37981 actttccaca tttaaagtct agtatgccta gcatgtggac taatagctat tttgtaggta
    38041 ctgttggttc tgtgagttta gaagtagtta aacaatatat agaaaatcaa caagttagga
    38101 caaagaaatg ataaaaactc atagagtgaa attaaaccta actagaacac aatttgaatt
    38161 agtaagggaa aagcaaatgg aaagtgctaa ctgttggaac tacatagtta acttatctaa
    38221 agaatactat tttgaacata aacaatggat taaaaagaat gatatacaaa agttaatcaa
    38281 aggtaagtac aatcttcata gtcaaactat tcaagctata tcagataaat ttgatgcaaa
    38341 tagaaagact atatcagaat taagaaaaaa aggtaatact aaagcaaaat atccatacaa
    38401 gactaagaaa ttttatataa taccttttaa agctagtgct attaatagaa atgctaaagg
    38461 caacttaaaa ttatcaatgt caaaaggcag gtatttagaa ttagatttta atgttgagaa
    38521 tataaaaact gctgaaatag tatggcgaaa tggatattat ttatactaca cttttgacaa
    38581 tgaattaaat ggagttgtag ccaaaggcac taataccgta ggtgtagact taggggaaat
    38641 acattcgata gctagtgtaa caaataaagg tgtagggctt attctatcta atagagaagg
    38701 cagaagtatt aaacaattta gaaataagat gtatgcttat atatctaaaa gattaagtaa
    38761 atgtaaaaaa ggtagtagac aatcaaagaa gttatggaga ttaaaaaata agataagaag
    38821 taaaacagat aatcagttaa tgaatttata tcatcaaaca actagaaagt ttatagactt
    38881 ttgtgtagag cagaaggtat gtgaaatagt tcttggggat ataaaagggg ttgaaaaaga
    38941 tactaagaaa aagaaaagat taaacagagt taatagacag aaaatatctc aaatggagta
    39001 tggtcgaata aaggactata taaaatataa agcaaaagag caaggtattg aagttaagtt
    39061 agttaaagaa aactacactt ctcaaacttg cccaaagtgt tctaaaaaac acaaaccaac
    39121 tggtagaact tatagttgta cttgtggtta tgaaactcat agagatatag ttggtgcttg
    39181 gaatatttta aataaaaagc ataaatatgg tttagtagat tttagaataa atcataagca
    39241 accaataaat ttaaaagtat ctaccgtttg agccatgtgt gggcgaataa ctttaaggta
    39301 gtagtggcga agacaccgtc cgaagtagct tggctttgta accacctctg ttgtactgtt
    39361 taagtataaa taagtgtgtt agccaccaag aatccccttg ctttagctat ggggagtgtg
    39421 tcaataaaat actttaatga aaaatagcta actttaattg taggatattc ttaatatatt
    39481 tgaaataaat aaaaatattg aaggatataa tattcaattg gtatataaat aataaatgat
    39541 agttaaattt taccttagag gctaattagg tgtttagtca aagttaatgc ctttagaata
    39601 caaatgtatt ctaaaggcat ttttactatg tattttcatt tatattacta aacattatct
    39661 atttttatat acatttctat gtattttatt ttcatcaaat cggtctataa actcattttt
    39721 ttcttcacta tatccaatag atataagtgc ataaggaact aattttaatg gtaagtctaa
    39781 agtttgtcta actttattta ccactttatt tttaggaaat actcctgccc aacaagcagc
    39841 tagaccttga ttagtaactt ctaataataa attttgtgtg caagcaccta aatcttgtat
    39901 ccatttacca ggttttaaga atttatctct atttccaagc actactatac ataatggtgc
    39961 aaattctata tgctttgctc tatgctgtga ctttgagagc tgtttcagca gttccttatc
    40021 atctactata ataaattccc aaggttgagc attttttgat gaaggtgctt gcatagctga
    40081 ttttaatatt tcagttattt tatcttcttc tatattttta tctatatatg ttcttattga
    40141 acgtctattt tttataactt ctaacataca gattctcctt taaataaaat tatttctatt
    40201 tatataatat cattacttta aattttagct attacttata taaagaataa actttgtgtt
    40261 ggttttatct aagtaaaagt aacattcaga actaaaaaac a
//
LOCUS       DAFKMX010000011        40258 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFKMX010000011 DAFKMX010000000
VERSION     DAFKMX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.011"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            234..1109
                     /gene="hslO"
                     /locus_tag="LZB08_000471"
     CDS             234..1109
                     /gene="hslO"
                     /locus_tag="LZB08_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBM7313990.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIITTSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            1500..2300
                     /locus_tag="LZB08_000472"
     CDS             1500..2300
                     /locus_tag="LZB08_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBM7313991.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            2828..5068
                     /locus_tag="LZB08_000473"
     CDS             2828..5068
                     /locus_tag="LZB08_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBM7313992.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            5482..7017
                     /locus_tag="LZB08_000474"
     CDS             5482..7017
                     /locus_tag="LZB08_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBM7313993.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQLAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            7033..7794
                     /locus_tag="LZB08_000475"
     CDS             7033..7794
                     /locus_tag="LZB08_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7313994.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            8132..9001
                     /locus_tag="LZB08_000476"
     CDS             8132..9001
                     /locus_tag="LZB08_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7313995.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            9330..9581
                     /locus_tag="LZB08_000477"
     CDS             9330..9581
                     /locus_tag="LZB08_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBM7313996.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            9693..10472
                     /locus_tag="LZB08_000478"
     CDS             9693..10472
                     /locus_tag="LZB08_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBM7313997.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLEFENDVDEEMNWIITRAN
                     ELIKKYPSQKEMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(10607..11257)
                     /locus_tag="LZB08_000479"
     CDS             complement(10607..11257)
                     /locus_tag="LZB08_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7313998.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(11254..11757)
                     /locus_tag="LZB08_000480"
     CDS             complement(11254..11757)
                     /locus_tag="LZB08_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7313999.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            12079..15132
                     /locus_tag="LZB08_000481"
     CDS             12079..15132
                     /locus_tag="LZB08_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBM7314000.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTTVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGETAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKSTQDE
                     VFAGAKAFVKENKGRYECGSYIYSGEGQELGQFWAKLNVGNTKLQKVSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDIVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKGNLPAEATRTWVTKTIAETDSDGTTHYITKLTDAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSFDLLPYGKFRIVESEAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELKCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELLIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYQLKGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVAEHKDIEDEGQTITFKEKPEVPEKPENPETPQTPE
                     KPSRPSDSPKTGDNTNLYGLLALLMTSGAGLAGIFFYKRRKMKKL"
     gene            15370..15696
                     /locus_tag="LZB08_000482"
     CDS             15370..15696
                     /locus_tag="LZB08_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7314001.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFEKDKNNRRVLTRRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLLADDI
                     VAVEEK"
     gene            15712..16101
                     /locus_tag="LZB08_000483"
     CDS             15712..16101
                     /locus_tag="LZB08_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7314002.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNNNTQGQPKKQN"
     gene            16171..16587
                     /locus_tag="LZB08_000484"
     CDS             16171..16587
                     /locus_tag="LZB08_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBM7314003.1"
                     /translation="MKVLVSSCIMGKNCKYNGGNNLNPKVVEFLKDKEVIEICPELLA
                     NMPTPRPSAEIVNGIVMDINGNIVDEEYRHAVQLALNEIRSMEIDLVILQSRSPTCAV
                     KNIYDGSFTGKLIKGQGLFAQALIEAGYKVLDSEDF"
     gene            16661..18028
                     /locus_tag="LZB08_000485"
     CDS             16661..18028
                     /locus_tag="LZB08_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314004.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLLVFVAVLLLLNTKQFM
                     RTDWEHFSLLENGLTLSPYNFITIGIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     VLENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAILYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GAKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQEIGRLEEKRT
                     ASNES"
     gene            complement(18034..18234)
                     /locus_tag="LZB08_000486"
     CDS             complement(18034..18234)
                     /locus_tag="LZB08_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314005.1"
                     /translation="MQVYIDLENLLKEKNISKNKVCEACKLQRTQLNNYCKNKVSRID
                     LTILAKLCDFLECSPNDILKLK"
     gene            18346..18756
                     /locus_tag="LZB08_000487"
     CDS             18346..18756
                     /locus_tag="LZB08_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314006.1"
                     /translation="MCKKRKIITITVLFVLGVAVSYLQWGREIDVAGSIGTSSENFHE
                     EHVTFTLNRLIITDKEKCAEEIIKKCRENDFASTLFSYDVAKPNALYGTVYRSRFSMK
                     GQKPLFHFRYTQTADTLGSYNIVDDPEAFTLVIE"
     gene            18995..20191
                     /locus_tag="LZB08_000488"
     CDS             18995..20191
                     /locus_tag="LZB08_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBM7314007.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLALASGITREYLNKIESGK
                     MKPSKELLNILHEELARFNPEAPLTMLFDYVKIRFSTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSSDEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMDTIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            20204..20338
                     /locus_tag="LZB08_000489"
     CDS             20204..20338
                     /locus_tag="LZB08_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBM7314008.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            20339..20560
                     /locus_tag="LZB08_000490"
     CDS             20339..20560
                     /locus_tag="LZB08_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314009.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20631..21257
                     /locus_tag="LZB08_000491"
     CDS             20631..21257
                     /locus_tag="LZB08_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7314010.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKVNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFRKTDFINLCKSMMELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            21346..21642
                     /locus_tag="LZB08_000492"
     CDS             21346..21642
                     /locus_tag="LZB08_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314011.1"
                     /translation="MNDIFKDMQSKVGCDYISDLPSYKRKVWHEMKRLNLADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLNCR"
     gene            21561..22064
                     /locus_tag="LZB08_000493"
     CDS             21561..22064
                     /locus_tag="LZB08_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7314012.1"
                     /translation="MQETRVLVETRGTTGEETISYWFELPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            22082..22585
                     /locus_tag="LZB08_000494"
     CDS             22082..22585
                     /locus_tag="LZB08_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7314013.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHFGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            22702..23100
                     /locus_tag="LZB08_000495"
     CDS             22702..23100
                     /locus_tag="LZB08_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314014.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            23078..25528
                     /locus_tag="LZB08_000496"
     CDS             23078..25528
                     /locus_tag="LZB08_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314015.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHTLQIATESSVRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKNYKKE
                     TLIKYYDLIRPTRCVIEENQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDLSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQAILLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPVSRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDLGEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            25528..27741
                     /locus_tag="LZB08_000497"
     CDS             25528..27741
                     /locus_tag="LZB08_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBM7314016.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTANGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YMFMRHRARRMERRMARAVSHGSMVGAGTGAVTGAMLSGNGSSRSNPQKDKRTFSRSN
                     TSSSFGNRAGSAVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSD
                     FKRGMVQEQQSRQTGRLEKQEQHRQNIADKRMELQKAQEARHRKTDGSATTGATRPHE
                     RPATASTLSKPSAEKTQEVKRPATATTSKASEPVKTNVIKERPLSSGASDRKASQSAQ
                     IAHRQNVEKVVSQETRQNYTKDRRTKVQQTQSVQKNQQTTENPRNLVTKKGQKKK"
     gene            27738..28745
                     /locus_tag="LZB08_000498"
     CDS             27738..28745
                     /locus_tag="LZB08_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBM7314017.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKHQGIDDLNVSIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            28761..29672
                     /locus_tag="LZB08_000499"
     CDS             28761..29672
                     /locus_tag="LZB08_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314018.1"
                     /translation="MFKKNKKQTETVKEPKEKKVRTVKVGTHKKTMIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVIEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(29719..30276)
                     /locus_tag="LZB08_000500"
     CDS             complement(29719..30276)
                     /locus_tag="LZB08_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7314019.1"
                     /translation="MKTTKEAVIKATSDLADEKGLNNVSLKAVAQKLNIRTPSLYNHI
                     ESLDNLLLEVAHNGMRDMNERMMKIAVGKIGKEAIKLVSIEYLNYMIEHPGVYETIQW
                     AVWHGTEETATIFNNYLSLLTTLIQSCSLNKDKTLEILNMLTGIIHGYTTLQLGNAFS
                     ASDKVRFELAEAIDTLLVGIFQKYK"
     gene            30454..31149
                     /locus_tag="LZB08_000501"
     CDS             30454..31149
                     /locus_tag="LZB08_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314020.1"
                     /translation="MCTRFVYNGIDKIVGFNFDIDLSVWDHTVITEENRFYIGIKMPD
                     GLYHSFHGINKNGNVGTLLYVHGNENARYIESEDCITISELTEKFIKAEISFDKALDI
                     VKKKKIIYAPDATMQAMLSDKKGRVLIIEPGIGYRYEQENFSLITNYSILKPDITKPY
                     IVSGDTRYEVAKELLAGYDKDFSVNDAFKVLKSVSQKDLWATRVSFVYSTEKNKVYYV
                     LNNDFSNVFEFQF"
     gene            31195..31419
                     /locus_tag="LZB08_000502"
     CDS             31195..31419
                     /locus_tag="LZB08_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314021.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTAP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKI"
     gene            complement(31465..32757)
                     /locus_tag="LZB08_000503"
     CDS             complement(31465..32757)
                     /locus_tag="LZB08_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314022.1"
                     /translation="MLNNLYRKLHILFASSVMLIITLVISFVVANTVHTEKINESTLF
                     QRLTTLLIYQVESASSDMDKALKTYEESYHIFSLITDTRGNTIYQSDFPFPTSADNLL
                     HDVEKQISTQPLSQIENSTTSQGGFLEIRGTHHDTYFVIPATIRTANDTVYHATFLYQ
                     TASLTDILQKTLPIYLLIWFLACIVVIVLTRYLLKKSFAPTERILQSQKDFVATASHE
                     LKSPLAVMISNTDMLLDNVSLNEQAKQAVQTIDFECMRLSRLVKDMLLLASSDAKTWT
                     LHKSTINVDTLLITLYETYEPVCMKQNLELKLHLSEESYPAMFTDKDRLFQILCVFMD
                     NAIQHSKNNSLIEIEVIATEKNIAFSVVDHGQGISDEDKQYIFDRFYSGDKSHTNKSN
                     FGLGLSIAKELTQMLNGIIIINDTAGGGATFTVKFPLK"
     gene            complement(32751..33437)
                     /locus_tag="LZB08_000504"
     CDS             complement(32751..33437)
                     /locus_tag="LZB08_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314023.1"
                     /translation="MKILLLEDDLELCKNIKISLEKENYMVDCCNDGETAMLYALNTD
                     YAYDLAIVDRMLPIIDGLTIIKSMRRKGISIPILIITAMSTLDNRVEGLDGGADDYLV
                     KPFHIKELLARVRALTRRPANIQLSSKLEYGDLTFDKDSRTLSTPVNSLLLTSKETEL
                     TFVFMQNPETLFSREQLILKIWGSSSEVETGNVDSYISFLRKRLKELKSSCKITTVYG
                     AGYKLEKETC"
     gene            33600..34097
                     /locus_tag="LZB08_000505"
     CDS             33600..34097
                     /locus_tag="LZB08_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HBM7314024.1"
                     /translation="MKKTNISKFVAVGLCICALTGCGASPDEKPDTSNPIVNSNTNEE
                     NANGSSENKGNDILESANLIGSVLEFTDNGCSVSQAKETEGGASLQIEASGLENKDNA
                     VSVTYNPDCEFVVATLNKQLGSVTNITTGSISDVKKQSNVYLYGEFSDTLHFNATKVV
                     IARWE"
     gene            34109..35332
                     /locus_tag="LZB08_000506"
     CDS             34109..35332
                     /locus_tag="LZB08_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314025.1"
                     /translation="MKFYQLGFRYLQRKKGKTILLLIVLILVNSMILGTSMILRTTNE
                     SKQAMQEKTNSKIVAEITSKDSKITENEVNKIETLEDVSSINRIGRQEVFPNDFAPVT
                     LNTSTNEENLKIALLSYDDLEKDSPFSEMQYRLSSGDYIKHEKKGAVINANLANQNEL
                     KVGDTIELSTENGTKVSVQIIGIFMSAGNVEKDQPTETTAVNRIENQVFIDNSTYKEL
                     FKEAEFEKLCVYSKRPEKLDVLETSLQKIFGNDVELSTSDTLYQQMSAPLEQISKVAN
                     LMLVLTLVTGTVVVSLLLCMWMRTRQKEVAIFMSLGKTKSEIFLQTLLEAGSVFTLSV
                     LGATAIGKLFSGVLQNVITGSETVTENLKVVLQIQDIGMLFLLGGAVILVALCCSILP
                     ILRANPKDILAKMEG"
     gene            35336..36781
                     /locus_tag="LZB08_000507"
     CDS             35336..36781
                     /locus_tag="LZB08_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314026.1"
                     /translation="MNFFGLARRSVFRKPIKSILLLLIVFAISTLLLAGVASKNASIE
                     VQDKTRQAIGAGFLLERNAEYRTKRVREYSEKIGNDKEGSFGGYHQEKEIINGVETWS
                     GWTTNEFESLDIKDIEKLAKTKGIADYNITTATTPVNPVNFKRIEDKDVDQSVDEGGV
                     SLIGNKDMKLDRNVLSGNLHIKEGRMITPEDKDMCVISEELAKQNNLKIGDKISFNDY
                     HDTVNSKVSEAEIVGIYQVDQKMSPLMQGDTYRSENVIFTDLRFPEKAEGETSPLYER
                     AYFKVEDVEAYDEVKENLQKVDINWEQYDLIDNNGNSETMSSNFNDLAKVSEMMILVI
                     SVASFVILVLVFLFWLKNRVQEVGIFLSLGVPKFRIIGQIWSEAIMIAVLSLMLSFAV
                     APAVSKVTANYLVSQQVQQMQEEEKNNEGKVSTEYVAPKQDVQNISVEVTPEMYLLDG
                     VSVLVLITASVLVSGIVILKRNPKDILSEMS"
     gene            36795..37430
                     /locus_tag="LZB08_000508"
     CDS             36795..37430
                     /locus_tag="LZB08_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314027.1"
                     /translation="MLEVKNVSYAYKTKKDKTILNNVSATFEEGIFYTIVGPSGAGKT
                     TLLSLLAGLDTAVKGTVLCNGEDITKKGLNYHRKHNISLVFQNYNLIDYLTTVENVKL
                     GGSGNAEKLLEEVGIGKEYWQRNVLQLSGGQQQRVAIARALASDAHVLLADEPTGNLD
                     ETTADEIIALLKKTAHELGKCVVVVTHSKHLAEEADEILEIKNGAISFLSD"
     gene            37705..38145
                     /locus_tag="LZB08_000509"
     CDS             37705..38145
                     /locus_tag="LZB08_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314028.1"
                     /translation="MKPSELQEKTCHQFDCAIKTVIRHRAIDFYRAIRRSEKKEMPFA
                     EIMDDLLNSIGTEDTYSTDYVAVFEVCGEKVSIEDEQLARALKAIEERKRNIVLQYFF
                     IGDTDTYIGKMLDCSRANVTKCRIQALDKLKQLIEEEKEKCQKS"
     gene            38130..38363
                     /locus_tag="LZB08_000510"
     CDS             38130..38363
                     /locus_tag="LZB08_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314029.1"
                     /translation="MPKELTPTFTVISKACDGDETAISKILEFYDPYINRCCMRPFYD
                     DNNNLRLAVDLEMKGFIKEAIIRKILNFDIKRF"
     gene            38677..38880
                     /locus_tag="LZB08_000511"
     CDS             38677..38880
                     /locus_tag="LZB08_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBM7314030.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            38958..40148
                     /locus_tag="LZB08_000512"
     CDS             38958..40148
                     /locus_tag="LZB08_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7314031.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEGRLLAFMEKDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
BASE COUNT        13744 a         6367 c         8487 g        11660 t
ORIGIN      
        1 tatataaaat gcagttttat agattaaaag taaagtgatg atattttatg atttttattt
       61 tctaataatt ataaagtttt atagataaaa attaaaatat aataactatt gcttagtaaa
      121 attaaaataa aatgggtttt gcccaaggtt aagatagata agtatttata gattaaaatt
      181 aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata ttaatgagag
      241 attatgtact aagagcaaca tcaggaaatg gtcaagttag agcatttgtc gcaactacta
      301 gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca acagcagcac
      361 ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat gatagtgata
      421 aattgactgt aataataaaa ggtggaggtc caattggaac tattatcacc acatcagact
      481 ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa gactatccaa
      541 atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa gttataaaag
      601 acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga gaaattgcag
      661 aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta gctttaggtg
      721 ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa ttaatgccag
      781 atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta ccttctataa
      841 ctaacatgtt aaaagaagga aaatcaccag aggatatatt aaatattgtt ttggatggtt
      901 tggaacctaa aatacttgat acctgtgaag ttgggtttat gtgtgaatgt tcaaaagaaa
      961 gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcaaata atagaagaag
     1021 ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat agtgaagaag
     1081 agttgcttga aattttaaag gaaatgtaga ctataaaaat agttgtattg atataagtaa
     1141 tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat ttattaataa
     1201 tagacatatt tatattaaaa aataagttta ataaattttt aagcttaata aattttaaga
     1261 attaagaagc tttctggctg taatgtttgc aatgttgcag ttagaaggct tttttatttt
     1321 attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata atttgtatat
     1381 ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg aaattctatg
     1441 aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa attttaaata
     1501 tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg atatttttaa
     1561 ttgcaaaaaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat agtagaaaat
     1621 ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta tggggaggaa
     1681 gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg gtattagtat
     1741 cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaat ttaactagga
     1801 agcaaaagga agaacttaga aataacgagt ttaaatcagc tttgagagag cttggagtga
     1861 aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca cattatgaat
     1921 taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg acccatatag
     1981 cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa gttttaaaga
     2041 aacttaaacg tgagggtaag ataaaagatg acttgtattt tgttaagcca aaatatgtaa
     2101 agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat gactacaata
     2161 aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa atgtatggta
     2221 taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct gaacttacgt
     2281 ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata agttgattgg
     2341 aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt taaagataga
     2401 ggtttttaat gtttaaaatt ttaaagttaa gttgagtaat tgtatagaat tatttgtgaa
     2461 agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata ggcaagatac
     2521 tttttttatg taactaatac cattttgaga ttattaactt aagaatttta tataatttaa
     2581 cactcatata taatgatgat atttaggtag aatagatgta attacgctta aataaaataa
     2641 cggtattttt tataaaaatc caaaccctac actatactac cagataaaat ctacatgcta
     2701 taatttgacc tgtgtttata aaaaaaggag taatctaata aaatagtatt tcacttatgt
     2761 ataactaaca actattatta cgattttctc taaataattt aaagaaaaat aatgcaggag
     2821 gtaaaatatg aacaatgaat ttataatttt aaaaggttgc aaagagaata atttgaacaa
     2881 tatttcctta aaaataccaa aaagaaaaat aaccatattc acaggggtgt cgggttctgg
     2941 aaagtcatct attgtatttg agacaattgc aaaagaatca caacgccaac taaatgaaag
     3001 gtttagtaca tttgtaagaa gttttttacc taagtatgga gaggttaaag ctgattgtat
     3061 agaaaatttg tcaacaccaa ttatcataga ccaaagtaga cttggtggaa attcaaggtc
     3121 aaccctaggt actattacag atgtaaattc ctttttaaga gctttatatt caaggtttgg
     3181 tagtgagtat ataggtaatg ccaatatgtt ttcttttaat aatattgatg gtatgtgccc
     3241 acactgtcat gggcttggaa aaaagctagt tcctaatatg aaccaaattc ttgatatgaa
     3301 taaatcatta aatgaaggtg ctatattgct ttcagggttt ggagtgggtt cttggcattg
     3361 gaatatattc gctgaatcag gattttttga tattaataag aggatatgtg attattcaga
     3421 agtagaattg gaaaagtttt tgcatggtaa agcagaaaag attaagatag aaaatgctgg
     3481 tcaaactaac atgacatatg agggattaat ggttaaattt aatcgcctat acttaagtag
     3541 agagggagaa atatctgaag ctactaagaa aaaattgagt aaacttctta tagaagatag
     3601 atgtcctata tgtgatggaa gaagacttaa tgaaagagta tataagtctt taattaatgg
     3661 ttataatata gcagatttaa ctagtatgca aattgatgaa ttggctgaag ttattaaaag
     3721 tatagatgag ccagaagcac aacctttaat taaaggaata atagaaaagt taaacagtat
     3781 catagatata ggtcttggat atttaacact ggatagagag acatcttctc tatcaggagg
     3841 ggaatctcag cgtataaaga tggttaagta tttaaatagt aatcttgttg atttaatgta
     3901 tatttttgat gaaccaagtg taggacttca tccaaaagat gtgtataaat taaataatct
     3961 tttaaaaaaa ttgcgtgata aagggaatac aattatagtt gttgaacatg acccagatgt
     4021 tattaagatt gcagaccata ttgtagatgt aggaccaaag gcaggtaagt atggtggaga
     4081 agttgtgtat gaaggcagtt atgaaaatct acttacaagt ggtactttga cagggaatgc
     4141 tttaagtaaa tttcttccta ttaaagaaag tgtaagagaa catagtggat atttagaagt
     4201 taaaaattgt aataaaaaca atttaaaaaa tgtatcgatt aagataccta aaggtgtatt
     4261 gactctagtt actggagtag caggagcagg taaaagtacg ttgattaagg atgaattttt
     4321 aaagcaaaat cctagtgctg tacttataga ccaaagccct gtttcagcaa attcaaggtc
     4381 gagtttggca acatatagtg gaattatgaa caacataaga agcatatttt caaaaacaaa
     4441 tggcgtaaat gcttctttat tcagttcaaa ttcagaagga gcttgtgata actgtaaggg
     4501 ttctggaata gtagaaatga atcttgcttt tatggagagt ataaagagta cctgcaatgt
     4561 ttgtgaaggt aaaaaattta aaaaagaagt tttagaatat aggtttcaag ggaagaatat
     4621 aattgaagtt cttgaaatgt ctgttttaga ggcaattgag ttttttaatt taaaacagat
     4681 taaaacgaaa ttacaatgta ttgaaaaaat gggtattgga taccttactt taggacaaac
     4741 tcttgatact ttgtctggag gagagtgtca aagattaaag cttgctagtg aattaaataa
     4801 ggaaagttca gtgtatatat tagatgaacc aacaacaggt cttcatatgg ctgatattga
     4861 gaattttata agtataattg aagagattgt agataatgga aatacggtca taataattga
     4921 acacaatata gatgtaatta aaagagctga ttggattatt gagctgggac ctgaaggagg
     4981 aacaaaaggc gggagagtaa tatttgaagg tattccaaaa caattatgta acaataaaat
     5041 atcattaaca gcaaaatata tagtataata aataaaatat aataaaatta taatgatatt
     5101 ttaataaata taatgggtat ataaaatgat aattaaactt aacagtaata aataatgtga
     5161 gaaatattca aatttaatta aataaagtgt cagaaatttg tttaaatttt agtattttaa
     5221 cggttctgac cttttttctt atttttatgg agaatatcga ggatggagtc tctaatagtt
     5281 acttttttgt gtctacttgc aagtttgatt aaaaaatggc tatacacact tgtgacttca
     5341 gtcatgggag gttcagatta taatcttaat atataatttg tgaaataatt tgatgacgat
     5401 ttgctgatgt ataaacaata aagattaact acattaaaat ttaaatcttg ttaagtaaaa
     5461 cttataaaag gagagagatg gatgataaat gatattctaa ccttattatc tgaaagaagt
     5521 gattttttta taaagctatc catagaacat ttgatgattt ctattatttc tataataatt
     5581 gcgattgttt tgggtggtat aattggtatt attgtaagtg aatttcataa ttcttctaaa
     5641 cctatattgg gaactatcaa ttttctatac accattccat ctatttctat gctaggattt
     5701 ctgataccat tttctggaat tggaaatact actgctgtta ttgcacttac catttatgca
     5761 ttattgccaa tggttagaaa tacatatact ggaattacta atgtggataa aaatattctt
     5821 gaagcagcta ggggaatggg aagtacagat tttcagatat tgtataaaat aaaacttccg
     5881 ttagccctac ctgttattat gtctggtatt agaaatatgg tgattatgac aattgcatta
     5941 gctggtatcg cttcatttat cggtgctgga ggcctaggtg tagccatata tagaggaatc
     6001 acgacaaata acacaataat gacaattact ggaagtatac ttattgctct attagcttta
     6061 atatttgatt ttattttagg gctggtagaa aaattttttc aacgaaaaag aaaaaagaaa
     6121 aataggaaaa aagtttttgc cattctagga agccttattc ttatagtaat tatctttacg
     6181 tcttatttat ttaatccttc aaagactcaa actattcata ttgctactaa acctatgact
     6241 gaacaatata ttcttggtga gatgttggat attttaattg aaaaaaacac aggtctaaac
     6301 gtggaaatta cacaaggtgt aggaggggga acatctaata tccagctagc tatggagaat
     6361 ggagaatttg acatttatcc agagtataca ggtacaggct ggaatatggt acttaagaaa
     6421 aatgaattat atactgaaag tatgtttagt aaactacagt cggaatacaa aactaaactt
     6481 aaaatgcaat ggactggtat gtatggtttt tctaatagct atgcattagc agttagaaaa
     6541 gaagttgcag ataaatacaa tttaaaaaat ataacggact taaaagatgt atcttctaaa
     6601 ttgacatttg gagctgaata tgacttttat gagagagagg atggatatac tccattgtgt
     6661 aatacatatg gactgaactt taaagataca gttgatttag atattggatt aaaataccaa
     6721 gctatcaatg aaggtaagat tgacgtaatg cctatattta caactgatgg tcagtatagt
     6781 gtttcagatg ttgtagttct aaaagatgat aaaaatttct acccatctta tcaatgtgga
     6841 aatgttgttc gtttggaagt attagaggaa aatccagaac tatcaaaggt atttaagatg
     6901 gttggagata ttttgactga taaagaaatg gctaaaatga attatgacgt agaaaacaac
     6961 aatttagaac ctagagaagt tgctcgtgag tttctgaata agaaaggtct tttataaatt
     7021 ggaggaaata taatgacacc tattatacaa tttaaaaata ttaaaaaaca atataatgat
     7081 aaaactataa tagataatct taatttagat atagaaaaag gagagttttt gactgtaatt
     7141 ggttcgtctg gttctggtaa gacaacactc ttaaaaatga ttaatggtct gattttgcca
     7201 gatggaggaa atattttgat aaaccaaaca gatatcaaaa acgaggactt aattaaatta
     7261 agaagaagaa taggatattg tgtacaggga agtgttcttt ttcctcatat gactgttgaa
     7321 gaaaatattt catatgtacc aaatctccta agtaaaaaga ataaattaga agtaaaaagt
     7381 gctgtaaaca agtggatgga aatagtggga ttgccaaatg atatgaaagt gcgttaccca
     7441 tcagaattgt caggtggtca gcaacaacgt gtagggattg caagagcttt agcttcttct
     7501 ccagaaattt tattaatgga tgaacctttt ggtgcagttg atgagattac acgaaagcaa
     7561 ttacaaaaag agataaaaga aattcataaa aaaacaggag ttacgattat ctttattacg
     7621 catgatattt atgaagcttt gattttggga acaaagactt tggtattaaa tcatggagtt
     7681 gttcaacagt atgatactcc tgagaatatt ttaaatactc ctgctaacca atttgtagac
     7741 cagttattga atataagaaa atctataata gatgatgata atttaaaaga ataaatttaa
     7801 atttaaaatc aaattgttgt gataaaaact attgatttag agctaacttc aagttgtata
     7861 cttgaagtgg aaaagttaga ttaaggtata gaagggcatt tagcagttgg ctgtagacga
     7921 ggtaactgct gaaggtgctt tcaataaatg gctagaacct gtatctgttg aagattataa
     7981 taaattgaaa taagaagctc aatatacaga gtagataggc acttcaaata gaagtgtctt
     8041 ttgtgtgcca taaaataaat caaaatatta gcaaagaatg tgaaaaacaa aataaaataa
     8101 tatgctatct ttaaaatagg agggatgcta tatggataac aatttgatta ccattgaaaa
     8161 aactttagat tatatagaaa aacatctttt agatgagatg ttagatttaa atgtaatttc
     8221 taaagagata ggctattcaa aataccattt acatagaatg tttacatcag ttgtcggatt
     8281 tagtgtacat aattatatac aaagacgtag acttactgaa gcagcgagaa tgctaatttt
     8341 tacagaaaaa ccaattattg agattgcttt aatctcgggg tatgatacac aacgctcttt
     8401 ttcaagaggg tttaaaaaaa tgtttaaacg tagtcctaat ttttttagaa gacatcaaga
     8461 ttttttacca ctacaattaa aatttgatat tcataataga aaaaaattac gtggagatat
     8521 ggttttagat gttaaaaaag ttaattgtgc agatataaat attgttggct attctggtaa
     8581 agtaagaaaa ggatttcata ttattggaaa atgctggagg gatttgcata aaaataaaga
     8641 gatgatagaa aataaggtag atttaggttt tttaattgga gttagtgatt atagcaattt
     8701 taaagagatt gagaatactc ctacatttac atacattgca ggtgctcaag tagctacttt
     8761 tggcaaatta cctaatggaa tggaaaaatt cgtattaccg aaaagtaatt atattgtttt
     8821 tacttttatt gggaaaaatg aggatagtat gcaaaatgtt gtagaatata tctatcaaga
     8881 atggttccct aattcaagtt gccagtttaa tgaaaataat ttatatgact ttgtaaaata
     8941 tggagaaaaa actgatgaga atggtttgag tgaaattcag ttttgggttc caatcattta
     9001 aaagaaggta ttatgtataa agtagatgga tttatatagg caaagtttta cagcagtatg
     9061 tatttataaa taatttatag tttgtaggaa agaaataatt ttactataag attatgataa
     9121 aatacacaat actatgacaa aatagtgtta aataatagat ttaattttgc ataggaaaag
     9181 ttctataatg gtgtgtattc ataaataatt tataatctgt aggaaagaaa tagttttact
     9241 ataagattat gataaaatac acaatactat gataaaatag tactaaataa tagatttaat
     9301 tttgcaaagg ataaggtgtt ttaataaaaa tgaatgtaga aaacgaatta cttaaaaacc
     9361 tagatagatt gcatacaaca gagttaggcg ttgtaagaat taagaaaaat ctttctttgg
     9421 agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga atctccaaat gccattatta
     9481 atagaaaggg aaaaaactgg tatataagtg tatatgattg tattataaca gtaaacgctc
     9541 acagttatac aattattaca gctcataaag aaaagaaatg aattgcaaaa tacaaaggtc
     9601 aaaagcattt tccactgttt gtagtaccta tgaagaagta tataaaataa tattactttt
     9661 gaataataat ttaaattttg gaggttgaaa aaatgaatga tactgtaaaa gaaaaattgg
     9721 cagagtcact aacttctgat actactgata ttttacctta tttgccttat ttattacaag
     9781 atttttggga attaggaagt tcaccagaac aaatgttaca attatttaga aagcatatac
     9841 cttttaatga agatacaaaa gttttggatt tagcttgtgg aaaaggtgct gtatctgtga
     9901 agcttgcaaa agagttgaat atacatatca aaggtattga tttgatacct gaatttattg
     9961 attatgcgag aagaaaggct attgaatata aggttgatgc tttgtgtgat tttgaaatga
    10021 atgatgctaa tagttctgta aaaattgagc acgattatga ttgtgtgatt tttggagctg
    10081 taggggatat tttaggtaat tataaagaaa ctataagtaa gctattacct acaataaaaa
    10141 gtaaggggtt cattttaatt gatgatgcat atgtagtaga taaatctaca aatgaacagc
    10201 ttaattttga gagggtgtat cccacttaca atgagtggtg tatattgttc aaagaattag
    10261 gtctgaaact aattgaatgt ataccatgtt tagaatttga aaatgatgtt gatgaggaaa
    10321 tgaactggat tataactcgt gcaaatgagc taattaaaaa atatccttct caaaaagaga
    10381 tgtttgaaca atatgttatt agccaaaaaa atgaatatac tgatttacag caggaccttg
    10441 ttggagcaac ttggatttta caaaaagaat aagataaata ttagactaga taatcaatgt
    10501 tatagtgaat tataccatgt ctagtgaata taaataacat aaaaaaccgt caagatgaat
    10561 agtttattcg tgcttgacgg tttttagtat ttgttgaagt aaaattttat ttctttagta
    10621 tatcactttt agtcaatcca tttgatttag cctgagtatt ccattgatga ttccccataa
    10681 gtttatatag gaatctaggg aagttaggtg aaataaaaat agtacttttg ttggaatcat
    10741 ctatatgtat attatcacta agtatatttt ttgaaagttc ttttaatgca attcctaaat
    10801 cttttttagg tcctttaccc aaaggtacgg attctagtga accaaacatc tccccagctc
    10861 caattccaat accttgaacc catgtaaagt tgattttttt acaccaatgc tctatcattt
    10921 gaattgccaa atgattatgt tcaccttcat tgaatccaca gttaactatt gcatacactg
    10981 gaataggttt tttattttgt gtttttaaaa atttttcaca tttctctaaa aaatgaagta
    11041 aatgagatgg aataccatct acataaagag gaaatgctat tattaatcta tcacaattaa
    11101 gtatggcatt aagagcgtca ggatacttag atgtgctatc tactttaaat gtaaaaactt
    11161 tattatcatt ttgaataagt tgttttaatt cagataaaaa atactgtgaa ttatttttat
    11221 ttacttttgg actaccattt atcattacta tattcatatg gattcacctc ttatatcttg
    11281 tatattttca tagaaacaaa cagagtgttc ttttatattg aaatttatag catttccatt
    11341 tactaatttc gtagcagttt ctttttcagc ttctgtaata caatctccat agaatatagc
    11401 ttttaaattt atctgtttat catatctact tttatgacgc atcatattat cttttattac
    11461 aaagtatgga agaatataat taatgctttt atcaagtacg tttttgacaa aagggctaaa
    11521 ccctccgtaa cagcatctgc ttattataat aattccatca gattcattta taagtttacc
    11581 catgttgctg taataatctt ttacaacaca ttcagaagga gttttaatcc agcaaccaaa
    11641 acaaccaata catttgtgga tttcttttgt gtttggaagt atttttatat tgtcatcaag
    11701 tttgggtaat aacttttcta attctttgtc agttaaatca tgtattaaaa gcctcataaa
    11761 gtccatctcc ttttattatg tatgtttact tctaaaaaca caataaaaat ctgattatcc
    11821 actaaaacac agtatctatc agcattttca aggaaacaaa tacatcaatt tactacttat
    11881 ttactaccat tgaatgagcc ttgatacgca agttttccta gatatgcaaa gatatagtac
    11941 agcacatcag tattgaaaca agaaaaaatt gaatatttca tagactatgg aacgcataac
    12001 atgacgttcc ttttctattt ctagccgttc ttattggacg gctattttat tactcaaaat
    12061 gaaaggagca ttagatttat gaaaaagatg ttaaaacgat tgtgtacggg cttcttagct
    12121 cttgcaactg tcgttactgc tttaccaact acaaccgttc atgctgaaag caagcaatac
    12181 tggacggaaa gtgcagagcg tgtcggtatc attgaaaaag tgatgaatga cggttctatc
    12241 ggttctacat tcaatgaggg catgatgaaa gtcgagggcg aaactgccta ttgtattgac
    12301 atcaatacag attttaagaa cggctacaag accagagctg acgcaagctc acgcatgagt
    12361 gccgaccaga tttcagatgt ggcgttatcc ttagagtatg tcaaacagta tggcgaagct
    12421 cacaaggaac tgaactacaa acaagtttat ctgttggaac aatgcgttgt ctggcagaga
    12481 ttaagcgtac atcttggctg gcaatgtgat aacgtgcgag cttcttatga tgaaattcca
    12541 aaatctacgc aggacgaagt tttcgctggt gcaaaagcct ttgttaaaga gaataaagga
    12601 cgctatgaat gtggcagtta tatctactct ggcgaggggc aggaattggg acaattctgg
    12661 gcgaaactga atgtcggaaa tacgaaactg caaaaggttt ccagtaatac cagcattaca
    12721 gacggtaacg ggaattactc tattgcaggt gcgacctatg gtgtcttttc tgataaggac
    12781 tgcacgaaac agcttgccac ccttacgact gatgaaaacg gaaatacaga tattgtagag
    12841 gtaaaagcag gcacagtcta tatcaaggaa ttatccgcac cagcaggata taaagtggat
    12901 aaaactgtat attccttaaa ggttgaagct ggaaagacag caaccttgaa agtatcagat
    12961 acaccgaaag taacggacac tttgattgag cttttcaaga ttgatatgga aacacagaaa
    13021 gacaatccgc aggggaacgc ttctttagca ggtgcggaat ttacatggaa gtattatgct
    13081 ggcttctaca ataaaggaaa tctccctgcc gaagctactc gtacatgggt tacaaagaca
    13141 atcgctgaaa cagacagcga cgggacaacc cactacatca caaaattaac ggacgcatac
    13201 aaggtgtctg gcgacagctt ctatatgcag gacggcaaag cggttcttcc tttaggaaca
    13261 ctaaccgtag aagaaacaaa agctccaaac ggctacttgt tagagggtgc atatatgcag
    13321 gctggcgata agtccgaaca gattaagggc ttatatgtaa cacagattac cgaggacgga
    13381 gaccttgccg tactatctgg aagtaaccag ttttccgtat cagacaaggt tatccgtggc
    13441 ggtgtcaaaa ttcagaaacg agatttagaa acgggcgata caaagccaca aggaagtgcc
    13501 actttaaaag atactgcctt tgacatcatt tccttaaatg ataattctgt tttggttgag
    13561 ggaaagctat acaagaaaaa tgaagtagta aaaacaattc atacagatat tgagggtgtc
    13621 gcttctactt cttttgacct tttaccttat ggaaaattcc gcatcgttga aagtgaagcc
    13681 cccaacggtt acttaactga cggtgcaaaa ccgattgatt ttacaatcac agaaaatgga
    13741 aaaatcgtgg acttaactga cgaagctcat tctatctaca atcagattaa gcgtggagat
    13801 attgaaggcg taaaaatcgg agcaggtaca cacaagcgtc ttgctgatgt tccttttagg
    13861 atcacaagca agacgacggg cgaaaatcat gttgtggtaa ctgatgataa cgggcagttc
    13921 tccacttctg ctgactgggc ttcacataag cacaatacca acgcaggaaa gaccagtgag
    13981 gacggtgtgt ggtttggaac ttctgaacca gacgacagca agggtgcgtt accttatgat
    14041 acctacatca ttgaagaatt aaaatgtgat agcaacaaag gctttgagct tatcccaccg
    14101 tttgaaatcg tggtatcaag aaataacctt gtgattgatt tagggacttt gactgatgaa
    14161 tacgaaaaag aaatctctat ccataccaca gcgaccagca aggacggaga aaagacaatc
    14221 cttgcaggaa aagagattac gattgttgat actgtcaaat tggacggact tacaaaaggc
    14281 acaaagtatc agctcaaagg ctggcagatg ttaaaggaag aaaacgcaga acttcttatt
    14341 gacgggaaac gtgtagaaaa cgattatacc tttgtcgctg atgatgaaga aatgaaagtg
    14401 gaaatttcct atacattcaa tgcgtctgct ttaggtggca agaaccttgt tacctttgaa
    14461 gaattgtatg atttcagcaa tccagacgaa cccgtgaaag ttgcggaaca taaagacatt
    14521 gaggacgacg ggcagaccgt acttatcaca gagcgtatca tcaaaattca tacgactgct
    14581 accgataaag acggcaataa agaacttgaa gcaggaaaag acgttacaat cattgatacc
    14641 gtaaccttag agggcttaga agtcggcaca aaatatcagt taaagggctg gcagatgttg
    14701 aaagaagaaa atgcagagct tcttatcaat ggaaagcgtg tggaaagtga ttataccttt
    14761 actgctgaca gtgaaactat gaaagtggaa gttgctttta cgtttgacgc tacttctctt
    14821 gacggcaaac agcttgtaac atttgaggaa ttatacgatt taagcaatcc agacgagccg
    14881 aagaaagttg ccgagcataa ggacattgag gacgagggac agacgattac ctttaaggaa
    14941 aagccagaag tcccagaaaa accagagaac cccgaaacac cacagacacc agaaaagcct
    15001 agcagaccga gcgacagtcc caaaacgggc gataacacca acctttacgg actgcttgca
    15061 cttcttatga cttctggtgc tgggctggca ggtattttct tctacaaacg ccgtaaaatg
    15121 aagaaattat aaggcggtaa cggtatgatg aaagaaaaat cacgctctcc gccgaagctg
    15181 tcaaacggca gacttccgct tattctggct tgtctgccta caacctgcaa gaacacggat
    15241 agtcaagggt gcgaagcata cattttaccc tttactatct gtgggattgc tggtatttcc
    15301 cgaaccgcca gaataagaaa tcacaatcaa ttaacttatc aaaaatagaa agaaacgagg
    15361 taaaacacta tggaaatgaa atatgtcgtg ccagatatgg cacagtcttt tggaactctt
    15421 gaatttgcag gcgaaagcga gccagtcttt gaaaaagata aaaataaccg cagggtctta
    15481 accagacgaa gctataacct ttattccgac gtacagaaag gcgaaaatgt tgtggtggaa
    15541 attcccgtgc aggctggcga aaagcatttc aagtatgaac agaaagtaaa acttgtcaat
    15601 ccgaagttat acggcagagg ttacgcaatc ggggatatgg gacataccga ttatgtgttg
    15661 cttgctgatg atattgtagc agttgaagaa aagtaaagga gtgaagaatt tatgagatta
    15721 tcaaacgggt ttgtcattga caaagagaaa acatttggag aattaaaatt tacagcggtg
    15781 cgtgatgtgt tcttgcagaa tgaggacggg acaccaagta cccagcttaa aaagcgtatc
    15841 tatgatttga agtgcagtct gcatggtgga attatccccg tgtccgtacc gccagaagta
    15901 ccgttaagag aatttccgta caatgcagtt gtggagcttg tcaatcccgt agcagatacg
    15961 gtatcaagaa agacgtttgg tggtgcagat gtggactggt atgtaaaggc agaggatatt
    16021 gtgttgaaga ataaaggcaa ccagaacgca ggcaatccac agaataataa cacgcaggga
    16081 cagccgaaaa aacaaaatta gagattagat ttttattgtg aggtaattcg ttgaatgata
    16141 taattaggat aaattgtgag gtgcttatct atgaaagtat tagttagttc atgtatcatg
    16201 ggaaaaaact gcaaatataa cggtgggaat aatctcaatc cgaaagttgt agagtttcta
    16261 aaagacaaag aggttatcga aatttgtcca gaattattag cgaatatgcc tacaccacgt
    16321 cctagtgcgg aaatagtaaa tggtattgtc atggatataa atggaaatat tgttgatgag
    16381 gaatatcgtc atgcggtaca gttggctcta aatgaaataa gaagtatgga aatagattta
    16441 gttattctgc aatcaagaag cccaacttgt gcagtcaaga atatttatga tggtagtttt
    16501 acgggaaaat tgataaaagg tcaaggtctg tttgcacaag cgttaattga ggctggatat
    16561 aaagtgttgg actcagaaga tttttaatga atggtaatga aaaagcagtt agtcttagga
    16621 ttgactgctt ttttcgtacc cagaaaggag tgattgattt atgcgtaaga tttggaataa
    16681 aggacaccgt atcagagcca gcgacaagca ccttgtttat catttttcca tagggacgct
    16741 tctgttggta ttcgtggcgg ttctcctgct actgaataca aaacagttca tgcgtaccga
    16801 ttgggaacat ttcagcttgt tagaaaatgg cttgacgctt tccccttaca acttcataac
    16861 catagggata gcgactggtg tttgtgcatt ggtcgctttt ctgtattacc gcttctgtta
    16921 cgacagtttc aagaagctcc tgcaccgtca aaagctggca agaatggtac tggaaaataa
    16981 gtggtatgaa gctgataccg tacaagacag cggttttttt actgacctgc aaagcagatc
    17041 aagggaaaaa atcgtctggt ttccaaagat ttattatcaa atggaaaaag gcttgcttca
    17101 tatccgctgt gaaatcacat taggaaaata tcaagaccag cttttgcggt tagaggataa
    17161 gttagaaagt ggattgtatt gtgagctgac cgacaagacc ctgcatgacg gttatatcga
    17221 atataccctg ctttatgata tgatagcaaa ccgcattacc attgatgaag taagggcaga
    17281 aaacggctgt cttaggctga tgaaaaatct tgtctgggaa tatgacgcac tccctcacgc
    17341 tctgattgca ggtgggactg gtggtggtaa aacctatttt ctgctgacgc tcattgaagc
    17401 cttactgcat accaacgcta tcctttatat cttagaccca aagaacgctg accttgcgga
    17461 cttagggaca gttatgggaa atgtgtatca caccaaagaa gaaatgattg attgcgtcaa
    17521 tgccttttat gagggcatgg tacagcgaag cgaggaaatg aagcgacacc cgaactataa
    17581 gacgggcgaa aactacgcct atctgggact gccaccctgc tttcttatct ttgatgaata
    17641 tgtggcgttt tttgaaatgc tgggggcaaa ggaaagtgtg agcctgctta gccagttaaa
    17701 gaaaatcgtt atgttagggc gacaagcagg atatttcctt atcgttgcct gccagcgtcc
    17761 agacgcaaag tatttttcag acggtatcag agataacttc aatttccgtg tgggattggg
    17821 gcgtatcagc gaattaggtt acggtatgct gtttggttca gatgtgaaaa agcagttttt
    17881 tcagaagcgt atcaaggggc gtggttattg tgatgtggga acaagcgtta tcagcgaatt
    17941 ttacacgccg ttagtcccaa aaggacatga ctttctacaa gaaatcggca gactggaaga
    18001 aaaaaggact gcaagcaacg aaagctgaca gtcctacttc aatttcaata tgtcgtttgg
    18061 ggaacattcc agaaaatcac agagctttgc aaggattgtg aggtcaattc tggaaacctt
    18121 gttcttgcaa tagttattaa gctgtgtccg ctgtaattta cacgcttcac agactttgtt
    18181 tttgctgata ttcttctctt tcaaaagatt ttctaaatca atatatacct gcatacgctg
    18241 tacctcgtct tttctttgaa ttataagata caactgtatt gataaataag atgtattacc
    18301 gtagactgta ttataataca ccctacaaga aaggacagta acagtatgtg caaaaagaga
    18361 aaaataataa caatcacggt actttttgta ctgggagtgg cggtttccta tctgcaatgg
    18421 ggacgtgaga ttgatgtagc aggaagtata ggcacttcat cagaaaactt ccatgaagaa
    18481 cacgttacct ttacgctcaa ccgtcttatc ataacagaca aagaaaagtg tgcagaggaa
    18541 atcatcaaaa aatgcaggga gaatgatttt gcttctactc tgttcagcta tgatgtggca
    18601 aagccaaacg ctttatatgg tacggtgtac cgttcccgtt tttccatgaa aggacaaaag
    18661 cctttgtttc acttccgata tacgcagaca gcggacacgc tcggttcata caacattgtt
    18721 gatgatccag aagcgttcac gctggtaatc gaataatgca aggcaggacg ggacggcgac
    18781 gtgcgaagcg aaaggcgacg gcacggacta gccgttgctg gtgtggcgta agccacgcca
    18841 gcaggtaaaa cccctcggca tctaacagag gggtacgttt gcaaatagac aatagacaaa
    18901 aaacatagga aacataaggc ttctgacatg gtttcctagc aaaaatcggc tgtgaagtcg
    18961 ccttaaaaaa cttcacacgt tacagattgg gggtatggtt ctgaatgaag aacaatggat
    19021 aaaagaatta cgggagaaac ggattgctta cggtatctca caaggcaggt tggcgttagc
    19081 ttctggcatt acaagagaat acctcaacaa gatagaaagc ggaaaaatga agccgtcaaa
    19141 ggaacttctt aatattctgc atgaggaact ggcaaggttc aatccagaag caccgcttac
    19201 catgctgttt gattatgtga aaattcgttt ttccacgctg gatatacagc acatcatcaa
    19261 agatatatta aaactgaata tcaattatat gctccatgaa gattacggac attacagtta
    19321 tacggagcat tactctttag gggacatctt tatctatacg tcgtctgacg aagaaaaagg
    19381 tgtcctttta gagttaaagg gtcggggttg ccgacagttt gaaagttacc tgctggcaca
    19441 gcaaagaagc tggtatgact tcctcatgga cgcactcata gacggtggcg tgatgaagcg
    19501 tatcgacctt gctatcaacg accatacggg cattttggat attccagagc ttgcggaaaa
    19561 atgcaggaaa cgggaatata tcgggaagtc cagaagttat aagttctacc agtcgggcga
    19621 gcttatcaag cacagagagg acgacagaga atatatgggg cgtacccttt atcttggttc
    19681 gctgaaatct gatgtatatt tctgtatcta tgaaaaggat tatgagcagt acgtcaagct
    19741 ggggacacct ctggaagaag ccgacattat caaccgtttc gagatacggc ttcgcaatga
    19801 acgtgcctat tatgcagtac gagatttgct gacctattat gacgcagagc agaccgcctt
    19861 ttctattatc aatcagtatg tgcggtttgt tgatgaagaa ccagacaagc gaaaaaatga
    19921 ctggaaactc aatgaccgct gggcttggtt tatcggcgat aacagacaga gtttgaagct
    19981 gacgacaaag ccagagcctt acaccttaga ccgcacattg cgttgggtac aaaggcaggt
    20041 agcaccgacc ttgaaaatgc tgaaaaagat tgataaagga aacggtacag actacatgga
    20101 tacaatcgaa cagcaggcaa agctcacaga aaagcatgaa atgataatca aacagcagac
    20161 gacccctgca aaagatttag tagaaagtta ggagtgatga aaaatgtggg tattattgaa
    20221 agatttcctg ctggtatcta tgggaatggg tattggcgta gtcttgatgt gtattttgaa
    20281 tgttggcaaa gaagctgact ataaaatgaa acaattagaa gaaagcgagg acaattaaat
    20341 gaatttcgga caaaatttat atcaatggtt tttatcaaac gcacagagct tagtgcttat
    20401 ggcgattgtg gttatcggta tctacttagg tttcaagcgt gagttttcca aacttatcgg
    20461 cttcttggta gttgccttga ttgctgtcgg cttggtattc aatgctggcg gtgtgaaaga
    20521 tgtactgtta gagctgttca ataagattat cggtgcataa aattttattg tggtgctgat
    20581 atgcgaatgg tataatgtgg atatgaaaca cagtatctag gagtgatgaa atgaaatata
    20641 taatgcttca tggtttggga caatcttctg aaagttggag tgataccgta aaagtattca
    20701 atgatgattt tgaagtttta tgccctaatt tatcagattg gctttctggg aaaacaccat
    20761 gttatgatac attgtatcgg acattggaaa catactgtga acagtttgaa gaaccgctaa
    20821 atatttgtgg actttcttta ggtggtattc ttgctatgca gtatgcgata gaacacccag
    20881 aaaaagtgaa ctcattagtg ttaataggaa cacaatatga aatgccaaag tatctgttaa
    20941 aaatgcaaaa tctgatattt cgtattatgc cttgttcagc gtttgaaaaa atgggctttc
    21001 ggaaaacaga ttttatcaat ctatgcaaat ctatgatgga acttaatttt aaacatgaac
    21061 tgaaaaaaat atcatgttcg gtacttgttg tctgtggcga taaggataag ataaataaaa
    21121 aagcttcttt agaattgcaa caacagatag aaaacgcaga aatagctatt attgaaaatg
    21181 caggacatga agtaaataca gataatccaa cactattagg gcaaaagtta aatgtatttt
    21241 tcaaacaata tgtctgataa acaatttcat atccctacac acaaagcagt tagtcttagg
    21301 attgactgct tttttcatac ttaaatttca gattggagtg attacatgaa cgatattttt
    21361 aaggatatgc agtcaaaagt cggctgtgat tatatttctg acctgccctc ttacaagcgt
    21421 aaggtatggc atgaaatgaa acgactgaac cttgctgact atgaagaaag acagttagaa
    21481 gatttttcta aatatgtgtt tggtatgtcg taccagacct taaaagatgt gatgaaacaa
    21541 cagaaaggac gtgaggaaca atgcaggaaa caagggtgct ggtggaaacg agaggaacaa
    21601 ctggcgaaga aacaatatca tactggtttg aactgccgat agatgttgcc gagtttgaag
    21661 aaaagctagg tgtcggtgca gaaagtgggg attaccgtat tattgaaaag gtgttgcctt
    21721 atgctgatga agtccacgaa catacaagcg tgtatcagct caatgaatta gattttatgt
    21781 accgccagct ttcaagtgat atgcaggaag aatatgtatc actacttacg gtgtttgaaa
    21841 atttagaagc actttatatt tgcaggaacg tgattaccgt ttatccagac tgcaaaagca
    21901 tgatagatgt tgcaaggcaa aagctgatga acgacccgac atttaaacat ctgtccgagg
    21961 actgtcaaga atactatttc gattttgaag cctacgcttc tcacttacag gaacacggga
    22021 aatttttagt aacggaacac ggtatctttg aactgccaga gtaggaaatg aggtggtgct
    22081 tatgattgat gatatggcgg tttatattgc taatcttggc aaatacaatg agggctattt
    22141 agtcggtgct tggttcacgt ttcccattga cgaggaagat gtaaaagaaa aaatcggctt
    22201 gaatgaacag tatgaggaat acgctatcca tgataccgac aacttcccca ttgcgattgg
    22261 cgagtatgtt tccattgaag aactcaacga gatgtatgaa atgatagagg aacttcccga
    22321 ctatatcgta gagtgtctgg acgaatttat cagccacttt gggacgctgg aagaagtcgt
    22381 ggaacacaag gacgatattt attattatcc cgactgtgaa accatgacag atgttgccta
    22441 ctactacata gacgaattgc aggcactagg ggatattcca cccagcttgc agaactacat
    22501 tgactatgaa gcctacgggc gagatttgga tatgggcggt tgcttcattg aaacaagccg
    22561 aggtatgtgc gagataccat actaacgctg gaagatcagc gacaagcatt gttgctactg
    22621 acagcgtatt tctctttaag ggacagtctg aaaatggctg tccttttcca tttagaaact
    22681 tacgaaagga gctgaaagaa cttgaaaaag attaaatctt atacgggtat ctggaacgtg
    22741 gaaaaagtct tgtatgcaat caatgacttt aacttaccct ttcccgttac ctttacacag
    22801 attacatggt ttgtgattac ggaatttatc atcattctgt ttggggatat tcctccactt
    22861 tccatgattg agggagcatt tctcaaatac tttggtattc ccgttgctct cacttggttt
    22921 atgtcgcaaa agacctttga cggaaagaag ccgtacagct ttttgaaatc acagataact
    22981 tatgcgttgc gacctaaaat cacttatgca ggaaaagccg taaaactgca taagcagacc
    23041 ttgaatgaaa caatcacggc agtaaggagt gtgaactatg ttcccgataa aatatattga
    23101 caataacctt gtctggaaca aggacaatga ggtgttcgct tactatgagt tgataccgta
    23161 taactattct ttcctatccg cagagcagaa atttatcgtg catgacagct ttcgccagct
    23221 tatcgcacag tcccgtgagg gaaaaattca tacgttgcag atagcgacgg aaagctctgt
    23281 tagaagtatg caggagcagt caaagaagct ggtaacgggg aagttaaagg aagttgccta
    23341 tcagaagata gacgaacaga cggaagcgtt agtatctatg attggggaca atcaagtgga
    23401 ctaccgcttt tttcttggct ttaagctcat ggttacggaa gaacagctca atctgaagaa
    23461 catcaaaaaa tcggcgtggc tgacattcac agaatttctc catgaagtga accacacgct
    23521 gatgaatgac tttgtttcca tgccgaatga tgaaatcaac cgttacatga aaatggaaaa
    23581 gttactggaa aataaaatct cccgtcgttt taaggtgcgt cgcttggaaa tcaatgattt
    23641 tgggtatctc atggaacatc tttacggcag ggacggtatc gcctatgaag attatgagta
    23701 ccagctacca aagaagaact acaaaaaaga aacgctgata aaatattacg accttatccg
    23761 cccgacaagg tgtgtgattg aggaaaacca gcggtattta cgattggaac atgaggacaa
    23821 ggaaagctat gtgtcctatt ttaccgtcaa tgcgattgta ggcgagcttg attttccgtc
    23881 aagtgaaatc ttttattttc agcaacagca attcacattc cccgttgata cttctatgaa
    23941 tgtagaaatc gtggagaacc gtaaggcatt aacgacagtc cgcaacaaga aaaaggaatt
    24001 gaaagacctt gataatcacg cctatcaagc aggaagtgaa accagctcaa atgtggtgga
    24061 cgctttagac agcgtggacg agctggaaac ggacttagac ctgagcaaag aaagtatgta
    24121 taagttaagc tacgtcatac gggtgtctgc acccgatctt gacgagctga aacgtcgttg
    24181 tgatgaagtc aaggactttt acgacgacct caatgtaaaa ctggtgcgtc ctgctgggga
    24241 tatgctgggg cttcattctg aatttttacc tgccagcaag cgatatatca atgattatgt
    24301 gcagtatgta aaatcagatt ttcttgcagg gcttggtttt ggtgctaccc agcagttagg
    24361 ggaaactacg ggtatctata tgggctattc cgtcgatacg ggaagaaatg tgtacctgca
    24421 accgtccctt gccagtcaag gagtgaaagg cacagttacc aacgctttag cttctgcttt
    24481 tgtcggttcg cttggcggtg gaaagtcatt ctgcaacaat ctacttgtat attatgcggt
    24541 gctgtttggg ggtcaagcaa ttctgttaga ccctaaaagt gagcgtggca actggaaaga
    24601 aacgctccca gaaatcgccc atgaaatcaa tatcgtaaac cttaccagcg acaaggacaa
    24661 tgcaggactt cttgacccat ttgtgattat gaagaatgtc aaagacgctg aaagtctggc
    24721 aatcgacatc ttgacatttt taacgggtat ttcctctagg gacggcgaaa aattccccgt
    24781 acttcgtaag gcggtacgct ccgttaccca gagcgacagt cggggcttgc tccatgtgat
    24841 agacgagcta cgccgtgaag atacgccagt atcaagaaat atcgcagacc atatcgacag
    24901 cttcacggat tacgactttg cacatctgct gttttcagac ggtacggtgg aaaatgctat
    24961 cagtctggat aaccagctca acattattca agtagcggac ttagtattac cagataagga
    25021 cacgaccttt gaggaataca caactattga attgttgtcg gtgtctatgc tgattgtgat
    25081 tagtaccttt gctttagatt ttatccattc cgacagaagc atttttaaga ttgtcgattt
    25141 ggacgaagcg tgggcgttct taaatgtggc acaaggcgaa acactttcta acaagctggt
    25201 gcgtgctgga cgtgctatgc aggcaggcgt ttatttcgtt acccagtctg cctatgatgt
    25261 gtcaaaagaa agtctgaaaa acaatatcgg cttgaagttt gcctttcgta gtaccgactt
    25321 gggcgagata aagcagaccc tagaattttt cggcattgac aaggacgacg aaaacaacca
    25381 gaaacggctt cgtgatttgg agaacggaca atgcttattg caggacttat acgggcgtgt
    25441 cggcgtagta cagatacacc cagtctttga agaactgcta cacgcctttg ataccagacc
    25501 gcccgtacag agaaatgagg tggagtgatg aaagaaagga taaaaggtgc gttcacaaaa
    25561 aagaagattt tccactttct caaaatggct ctgttcgtgg tggcactctc ccttatcctg
    25621 ctttcacttt tggggacggt ggctcatgcg acggggcttg tggacgatac cataaacgca
    25681 gaaaatcttt actcaaaata tcccctttcc aactaccagc ttgattttta tgtggataat
    25741 agctggtcgt ggcttccgtg gaactggctg gacggtatcg gaaaatcggt gcagtacggg
    25801 ctttactgca ttaccaactt tgtctggacg ataagccttt atttaagcaa tgcaacgggc
    25861 tatgtggtgc aggaagccta taaactggac ttcatcaacg atatggcaga cagtatcgga
    25921 aagagcatac agacccttgc aggtgttacg gcaaacggtt tttccagctc tggcttctat
    25981 gttggtttcc tgcttctcat tatcttagtg gtgggacttt atgttgccta tacgggactt
    26041 atcaaacgtg aaaccagcaa ggcacttcac gctgttatca actttgtagt ggtgttcgtg
    26101 ctgtccgctt cgtttattgc ttatgctccc gactacatca aaaagataaa tgagttttca
    26161 tcagacatca gtaccgcttc acttgacttg ggaacaaaaa tcatgctccc caactctgac
    26221 agcgagggca aggacagcgt ggacttgata cgggacagct tattttctat tcaagtggaa
    26281 cagccgtggc tacttctgca atttggcaac agcaacgcag aggaaatcgg tacagaccgt
    26341 gtagaagctc ttgtatcggc aagtccagag gacgaggacg ggaaaaccag agaggaagtc
    26401 gtgaaaacag aaatcgagga caacgacaac aacaatctga cgataccgca ggtggtaaac
    26461 cgtttgggta tggtgttctt cctactgttc ttcaatttag ggataacaat atttgtattc
    26521 ttgcttacgg gcatgatgtt gttcagccag atacttttta tcattttcgc tatgttcttg
    26581 cctattagtt ttctgctctc tatgataccg agctatgaaa gcatggcaaa gcaggcaatc
    26641 gtgagggtgt ttaataccat aatgacacgg gcaggaataa cgctcattgt aacggtggcg
    26701 ttcagcattt ccagtatgtt ttataacata tccacggact atccgttctt tatggtagcg
    26761 ttcttgcaaa tagtatgttt cgctggtatc tatatgaagc tgggcgactt aatgagtatg
    26821 ttctcactta atgctggcga tagtcaaagc atgggacgac gcatattccg cagaccgtat
    26881 atgttcatgc gacatagggc tagaagaatg gagcgacgca tggctagagc tgttagtcat
    26941 ggaagtatgg ttggagctgg cacaggtgca gttacgggag ctatgctatc gggtaatggt
    27001 tcttcaagga gtaatcctca aaaagataaa cgtacttttt cacgaagcaa cacttcttcc
    27061 agttttggaa atcgtgctgg ttctgctgtc ggtgctgtct tagatacaaa aaataaagtg
    27121 aaagacaagg caaatgctgt caaagaaaac atcaaggata tgccgacaca gaccgcttat
    27181 gcggtatatt ccgcaaagga aaaggcaaag tccagcgtat cagatttcaa gcgtggcatg
    27241 gtgcaggaac agcagtccag acagacggga cgattggaaa agcaggaaca gcatagacag
    27301 aatatcgctg ataagcgtat ggagcttcaa aaggcacaag aagcaagaca tagaaagacg
    27361 gacggatcag cgacaacggg agctacccgt ccccatgagc gaccagccac agcttcaacc
    27421 ctttcaaagc cgagtgctga aaaaacgcag gaagtcaaac gtcctgccac agcgaccact
    27481 tcaaaagcaa gtgagccagt caagacaaat gttatcaaag aacgtccgtt atcttctggt
    27541 gcttctgata ggaaagcgag ccagtcggca cagatagcac ataggcagaa tgtagaaaaa
    27601 gtggtatcgc aggaaacacg ccagaattac accaaagacc gcaggacaaa ggttcagcag
    27661 acgcagagcg tccagaagaa ccagcagacc acagagaacc cccgtaacct tgtaacgaag
    27721 aaaggacaga agaaaaaatg aaactgaaac atatcgctct cattggcagt ctgtttccta
    27781 tcctcttttc tctggtgctt ttctttgggg tgttgattag tgcggacagc gacgacgaga
    27841 acagcaattt ttcttctggg attacgggta tgaacttatc cgcagaagtc ttgaaacatc
    27901 agcctatggt ggaaaagtat gccagagaat acggtatctc cgagtatgtc aatgtgctat
    27961 tggctatcat tcaagtagaa agtggcggta cggcagaaga tgttatgcag agttcggaaa
    28021 gtcttggttt accgcctaac tctttagata cggaaagctc aatcaagcag gggtgtaagt
    28081 attttgcttc cctgctttct tcctgcaaac atcaaggtat cgacgatttg aatgtatcaa
    28141 tacagtctta taactatggc ggtggttatg tgggatatgt ggcaggaaaa ggaaagaaac
    28201 acacctttaa ccttgcagag agcttcgccc gtgagaaatc gggtggaaag aaagtaacct
    28261 acaccaaccc gatagccgtt gcgaagaacg ggggctggcg atatggctat ggaaatatgt
    28321 tctatgtgga attagtcaat caatatctga ctgtggcaca ctttgataat gcgacggcac
    28381 aagcgattat gaatgaagcg ttgaaatatc aaggctggaa gtatgtgtat ggtggcagta
    28441 acccgaacac ttcctttgat tgtagcggac ttgtgcaatg gtgctatgga aaagctggta
    28501 tctccttacc gagaacagca caagcccagt atgacgcaac ccaacatctt ccactctcgc
    28561 aggcaaaggc tggggacttg gtgtttttcc attctaccta taacgctggt tcgtatgtaa
    28621 cgcacgtcgg catttatgta ggaaataatc agatgtacca tgcaggcgac ccgataggat
    28681 atgcagacct aagcagtagt tactggcaac agcacctaat cggtgcagga cgagtaaaac
    28741 aatagaaagg acttgaaaat atgtttaaga aaaataagaa acagacagaa actgtcaaag
    28801 aaccaaaaga aaaaaaggtg cgtactgtca aggtaggcac acataagaaa accatgattg
    28861 cgttgtgggt ggtgcttatc gcaagcgtga gctttggggt gtataagaat tttacggcta
    28921 tcgaccagca cacgacccat gaaaaagaaa tcattgaact tcgtttgcag gacaccaacg
    28981 ggatagaaaa tttcgtgaag aactttgcga agtcttatta cacatggagt aacagcaaag
    29041 aagcgattga agcaaggacg caggcaatca gcggttatct gacaaaggaa ttgcaagact
    29101 tgaatgttga tacaatcaga acggatatac cgaccagctc cacagttaca gatgtgattg
    29161 tgtggcatat cgagcaatcg ggaacggaca ctttttctgc tacctacgaa gtagatcagc
    29221 agataaaaga gggagaacag acaagcaatg tgaaagcgac ttatactgta aaagtccatg
    29281 tagacgctga cggggatatg gtaatcattc agaaccctac tcttgcacca gcaatcgaaa
    29341 aatcagacta tgagcctaag acaccagaag cagacgcaag cgtggacgct gatacggtaa
    29401 atgacgctac cgcatttctg gaaacattct ttaagctcta tccaacagcc acagaaaaag
    29461 agcttgctta ctatgtatct ggaaatgtga ttgagcctat cggcagggac tacctttatt
    29521 ctgaactggt aaaccctatc tttacaaagg acggggataa tgtgaaagtt aaagtagctg
    29581 taaaattcct tgataatcag acaaaggcga cacaagtatc acagtatgag cttgtgctac
    29641 ataaggatag caactggaag attgtaggat aaaagaaaaa ggcttgcagt tctgtattga
    29701 tttgcaagcc taaatatatt atttatactt ctggaaaatc ccgactaata aagtatctat
    29761 cgcttcagct aattcaaatc taactttatc tgaggcagaa aaagcgtttc ctaattgcaa
    29821 agttgtataa ccgtgaatta ttccagtaag catatttaat atttcaagtg ttttgtcttt
    29881 gttaagactg cacgattgga taagagtagt taataatgaa agatagttgt taaaaatggt
    29941 tgctgtttcc tctgttccgt gccacacagc ccattgaatt gtttcataaa ctccggggtg
    30001 ttcaatcata tagtttaggt attcgataga aactaactta attgcttctt tccctatttt
    30061 tccaacggca attttcatca ttctttcatt catgtcacgc ataccattat gagcaacttc
    30121 taaaagaaga ttatctaaac tttctatgtg attatataaa gatggtgtac gaatgtttaa
    30181 tttttgagca acagctttta acgaaacgtt atttaatcct ttttcatctg ctaagtctga
    30241 tgttgctttt attactgctt ctttagtggt tttcatagtt atgcacctca ttcaaaatat
    30301 catacaaaca caattctagc taattgaatt agtgtagtca ataacacaat tagaaaatta
    30361 tgattattta ttgactttct tacaaaaaca ttttaaaata ctaattagat tagttaaatg
    30421 actatttaaa atagtatttt gaggagtgat tatatgtgta caagatttgt ttacaatggt
    30481 atagataaaa ttgttggttt taattttgat attgaccttt ctgtatggga tcatactgta
    30541 attaccgaag aaaatagatt ttatattggt attaaaatgc ctgacggtct gtatcattca
    30601 tttcatggga taaataaaaa tggtaatgta ggaacacttc tttatgttca tggtaatgaa
    30661 aatgcaaggt atatagaaag cgaggattgt attacaatat ctgaattaac agaaaaattc
    30721 attaaggcag agatttcttt tgataaagcc ttggacattg ttaaaaagaa aaaaattatt
    30781 tatgcaccag atgctactat gcaagctatg ctttctgata aaaaaggacg agttttaatt
    30841 attgaacctg gaattgggta tagatatgaa caagaaaatt tttcacttat tacaaattat
    30901 tctattctaa aaccagatat aacaaagccg tacattgttt ctggggatac aagatacgaa
    30961 gtggctaaag agttattggc aggatatgat aaagattttt cagtaaatga cgcatttaaa
    31021 gtattaaaat ctgtttctca aaaagacttg tgggcaacta gggtttcatt tgtatattct
    31081 acagaaaaaa ataaagttta ttatgtcttg aacaatgatt ttagtaatgt atttgaattt
    31141 cagttttaat actaatgaat ttataaattt gcaattatga taagaaaggt tattatgata
    31201 ggactaaaaa agcgagatat aaaaaaagcg ttgaaagctg gtgcaaaagc tggcggtgta
    31261 tcattggctg atgttcgggc agatattgaa gcgacgattg atgaagctat gaatagcacc
    31321 gccccagaag tgcaggcaaa tttcaaaaag tattttggta ataaacgccc taccccagaa
    31381 gaatatattt acaagattac caaaaagaca aagatttgaa aaaaggcttg ctccatacat
    31441 agtgtatgat ctgcaagcct taatctattt cagaggaaat ttaacagtaa aagtagcacc
    31501 tccaccagca gtatcattga taatgataat accgttcagc atttgagtta attctttggc
    31561 gatacttagt cccagtccaa aattcgattt gttcgtatgg gacttgtcgc cactataaaa
    31621 tcggtcaaag atatactgct tatcttcatc agaaatgcct tgcccgtggt ctaccacaga
    31681 aaaagcaata tttttttcgg tggcgattac ttctatctct attaaagaat tattcttgga
    31741 atgttgaatg gcattatcca taaagacaca caagatttga aataatcggt ctttgtcggt
    31801 aaacatagca ggataacttt cctcggataa atgcagtttt agttctaaat tctgcttcat
    31861 gcagacgggt tcgtaggttt catataacgt aatgagcaag gtatctacat tgattgtgct
    31921 tttatgcaat gtccatgttt tagcgtcgga agaagccaaa agcaacatat ctttgactaa
    31981 acgggataac cgcatacatt caaaatctat ggtctgtacg gcttgctttg cctgctcgtt
    32041 taaggaaaca ttatcaagaa gcatatccgt gttggaaatc ataacagcaa gtggagattt
    32101 taattcatgg gacgctgttg caacaaaatc tttttggctt tgcagtattc gttccgttgg
    32161 agcaaacgac tttttcagca gataacgggt cagcacgata acaacgatac aagccaaaaa
    32221 ccagataaga agatatatag gcaatgtttt ttgtaaaata tccgtcaaac tggctgtctg
    32281 ataaagaaag gttgcatgat acacggtatc atttgctgtc ctaattgtag caggaataac
    32341 aaaataagta tcgtgatgtg ttcctcttat ttccagaaaa ccgccttgtg aagttgtgct
    32401 gttttctatt tgtgataatg gttgtgtaga aatctgcttt tctacatcat gcaacagatt
    32461 gtccgcagaa gttggaaatg ggaaatcact ctgataaatg gtattccccc ttgtatcagt
    32521 aattaaagaa aaaatatgat aactctcttc gtaggttttg agtgctttgt ccatatccga
    32581 agaagcactt tccacttgat aaatcagcaa ggttgtcaat ctctgaaata aggtgctttc
    32641 gttgattttc tctgtatgaa ctgtgttcgc aacgacaaag gaaatcacaa gggtaataat
    32701 cagcataaca gaacttgcaa acagaatatg aagttttcgg tataagttat ttaacatgtt
    32761 tctttctcca gtttgtaccc tgcaccgtag accgttgtta ttttacagga actttttaat
    32821 tcttttaggc gttttcgcag aaaagaaatg taactgtcta cattgccagt ttctacctcg
    32881 gaagaactgc cccagatttt aaggattagc tgttcacggg aaaatagtgt ttctgggttc
    32941 tgcataaata caaaagtcaa ctcggtttct tttgaagtta ataaaagaga attgacgggt
    33001 gtagataagg ttcggctgtc tttatcgaaa gttaaatctc catattctaa tttggaagat
    33061 aactggatat tggcagggcg tcttgttaat gcccgaactc ttgcaagcaa ttctttgata
    33121 tgaaatggct ttactaagta atcatcagca ccgccgtcta aaccctccac ccgattgtca
    33181 agggtactca tggcagtaat gataaggata ggaatagata ttccttttct acgcatagat
    33241 tttatgattg ttaagccgtc aataatcggt agcatacggt ctacaatggc gaggtcataa
    33301 gcatagtctg tatttaaagc gtacagcata gcggtttccc cgtcattgca acaatcaacc
    33361 atatagtttt ctttttcgag agaaattttt atatttttac ataactctaa atcatcttcc
    33421 agaagaagta ttttcataat gtatcaatcc ttttctatat aaattataga cagtataaca
    33481 gaaaagtctg taaaaatcct ctaaaaaatg atgatgattt tcttactttt ttacaggatt
    33541 tttagaatgg tttgctttta caataggggc atatcgaaca agaaaggtgg aatacaaaaa
    33601 tgaaaaaaac aaacatcagt aaatttgtag cagttggatt atgtatctgt gcattgacgg
    33661 ggtgtggtgc aagtccagat gagaaaccag atacgtctaa tcctattgtc aattctaata
    33721 cgaatgaaga aaatgcaaat ggttcttcag aaaataaagg aaacgacata ttagagtctg
    33781 ctaatttgat aggaagtgtt ttggaattta cagacaatgg ttgttccgta agtcaagcaa
    33841 aagaaactga gggtggagct agcctgcaaa tcgaagcgtc gggattggaa aataaagaca
    33901 atgctgtttc tgtaacctat aacccagatt gtgaatttgt tgttgcgaca ctaaataaac
    33961 agttgggttc tgttacgaat ataacaacgg ggtctatatc cgatgtaaag aaacaatcaa
    34021 atgtatatct ctatggagaa ttttcagaca cacttcattt caatgcaaca aaggttgtca
    34081 tagcccgttg ggaatagagg tgtgaagtat gaagttttat caacttggtt ttcgctattt
    34141 acagcgaaaa aaaggaaaaa ccattcttct gttgattgtt ttgatacttg taaacagtat
    34201 gattttaggt acaagcatga ttttaaggac tacaaatgag tccaaacaag ccatgcagga
    34261 aaagacaaac tctaagattg tggctgaaat tacaagtaag gacagcaaga taacagagaa
    34321 tgaagtaaac aagattgaaa cgctggaaga tgtttcgtct atcaaccgta tcggcagaca
    34381 agaggtattt ccgaatgatt ttgctcccgt tacgctcaat acttctacga atgaggaaaa
    34441 tttgaaaata gcgttgctct cctatgatga tttggaaaag gacagccctt tttcagaaat
    34501 gcagtaccgc ttatcttctg gcgattatat caaacacgag aaaaaaggtg ctgttatcaa
    34561 tgcgaacctt gcaaatcaaa atgagctaaa agttggagat acgattgaat taagtacaga
    34621 aaacggaaca aaagtatctg ttcaaattat tggaattttc atgtccgcag gaaatgtgga
    34681 aaaagaccag ccaacagaaa ctaccgcagt caatcgaata gaaaatcaag tctttattga
    34741 caacagcact tataaagaat tgttcaaaga agctgaattt gaaaaactct gcgtttattc
    34801 aaaaagacca gaaaaattag atgtgctgga aaccagcttg caaaaaatat tcggaaatga
    34861 tgtagaactt agcacttccg atacccttta tcaacagatg tctgctccat tagagcaaat
    34921 cagtaaggtg gcaaatctta tgctggtact tacattggta actggtactg ttgttgtttc
    34981 cctgctgtta tgtatgtgga tgcggacaag gcaaaaagag gtggcaatct ttatgagcct
    35041 cggcaagaca aaaagtgaaa ttttcctgca aactttatta gaagcaggca gtgtatttac
    35101 actttcggtt ttaggagcaa cagccattgg taaattgttc tcgggcgttt tgcaaaatgt
    35161 cattactggt tcagaaacag taacagaaaa tctgaaagtg gtgttgcaga tacaagatat
    35221 tggaatgttg ttccttttag gtggtgcagt tattctggtg gcattgtgct gttccattct
    35281 tccgatatta cgagcaaacc caaaagatat attagcgaaa atggagggat agtacatgaa
    35341 tttttttgga ttggcaaggc gttctgtgtt cagaaagccc ataaaaagta ttctcttatt
    35401 gttgattgtt tttgcaatca gtaccctgct tcttgcaggt gtggcgagca aaaatgccag
    35461 tattgaggtg caggacaaaa ccagacaagc cataggtgca ggctttcttt tagaaagaaa
    35521 tgcagagtac cgaacaaaac gagttcgtga atactcagaa aagattggta atgataagga
    35581 aggtagtttt ggtggctacc atcaggaaaa agagattatc aatggtgtgg agacttggtc
    35641 aggctggaca acaaatgaat ttgaaagctt agatataaaa gacattgaga aactggcaaa
    35701 aacaaaagga attgctgatt acaatattac aacagcaaca actcctgtaa accctgtaaa
    35761 tttcaaaaga attgaagata aagatgtaga ccaaagtgta gatgagggag gtgtaagctt
    35821 aattggaaac aaagatatga aacttgacag aaatgtttta tctggaaacc tgcatatcaa
    35881 agagggacga atgataaccc cagaggataa agatatgtgt gtcatttcag aggaacttgc
    35941 aaagcaaaat aatctgaaaa tcggcgataa gatttctttt aatgattacc acgatactgt
    36001 aaattcaaag gtatcagaag ctgagattgt tggaatttat caagtggatc aaaagatgtc
    36061 cccacttatg cagggagata cttaccgttc ggaaaatgta atatttacag atttgagatt
    36121 tccagaaaag gcagagggtg aaacaagtcc attatatgaa agagcctatt tcaaagtaga
    36181 agatgtggaa gcttacgacg aagtaaaaga aaatctgcaa aaagtagaca tcaactggga
    36241 gcaatatgat ttgattgaca ataacggaaa ttccgaaacc atgtcctcaa acttcaatga
    36301 tttagcaaaa gtaagcgaaa tgatgatatt ggtaatctct gttgcaagtt ttgttatcct
    36361 tgttttagta tttcttttct ggctgaaaaa tcgagtgcag gaagtcggca ttttcttatc
    36421 tctcggtgtt ccgaaattca gaattatcgg gcagatttgg agtgaagcga ttatgattgc
    36481 tgttctttcc cttatgctat cctttgctgt cgctcctgcc gtttccaaag taactgcaaa
    36541 ttatctggta tcacagcagg ttcagcaaat gcaggaagaa gaaaaaaata acgagggaaa
    36601 agtatccaca gaatatgttg caccaaagca ggacgtgcag aatatcagcg tagaggttac
    36661 gccagaaatg tatctgttgg acggtgtaag tgttcttgtg ttgataacgg cttctgttct
    36721 ggtatctgga attgtaatac taaagagaaa cccgaaagat attttatcgg aaatgagtta
    36781 ggaggacgac gaaaatgtta gaagtaaaga atgtaagcta tgcttacaaa accaaaaaag
    36841 ataaaaccat tttgaataac gtatcagcta cttttgagga gggtatcttt tataccattg
    36901 tcggtccgag tggtgcagga aaaacaacac tcttatcctt gttagcaggc ttggacacag
    36961 cagtaaaagg gactgtcctt tgcaatggcg aggacattac aaaaaagggg ctgaattacc
    37021 acagaaagca taatatctca ttggtatttc aaaattataa tctgattgat tatttgacaa
    37081 cggttgaaaa tgtcaaatta ggtggtagtg gaaatgctga aaaattattg gaagaagtcg
    37141 gcattggaaa agaatactgg cagagaaatg tattgcaact atctggcgga cagcaacagc
    37201 gtgtagcgat tgcaagagca ttagccagcg acgctcatgt acttttggca gacgaaccga
    37261 cgggaaatct tgacgaaact accgctgatg aaatcattgc tttgctgaaa aagactgcac
    37321 atgaattagg aaaatgtgtt gttgtggtta cacacagtaa gcatttggca gaagaagccg
    37381 acgaaatctt agaaattaaa aacggtgcaa tttcttttct gtctgattaa attagaatag
    37441 tgccgtaaag gagttgtcat tatggaatat tttagatata cctcgacgca atcagtttga
    37501 acctaacggc atttttataa caactgaata tagcacagtt ttaaaagctc gtacagacta
    37561 tatgttttgt gcgggctttt ttcatgccag aaaaaaattt tttaaagttt tttcgatttt
    37621 ggttacgctg tcccccactt tcaacgcggt atatatgaaa aggggcagac acctcttttc
    37681 gactagcgaa aggaggtgag gaaaatgaaa ccgtcagaac tacaagaaaa aacctgtcat
    37741 caattcgatt gtgctattaa aacagttata cgtcatagag cgattgactt ctatcgagcg
    37801 atacgtcgtt cagagaaaaa agaaatgcca ttcgccgaaa ttatggacga cctcttaaat
    37861 tccataggta cagaagatac atattctacg gattatgttg cggtatttga agtctgtgga
    37921 gaaaaagtat caatcgaaga tgaacaactg gctcgtgcat tgaaagctat tgaagaacga
    37981 aagcgtaata tcgttttaca gtattttttc attggggaca cggacactta cattgggaaa
    38041 atgcttgact gttccagagc gaatgtaacg aagtgcagaa tacaagcact agacaagtta
    38101 aagcaactga ttgaggagga aaaagaaaaa tgccaaaaga gctgacaccg actttcacgg
    38161 ttatttccaa agcctgcgac ggcgacgaaa cagcaatttc aaaaatcttg gaattttatg
    38221 acccatatat caatcggtgc tgtatgcgtc cattttacga cgataacaac aacttacgtc
    38281 ttgcggtaga tttggaaatg aaaggtttta tcaaagaagc gattattcgt aaaatcttaa
    38341 actttgatat taaacgcttc taattcatat cgggattgat tatgcccgtc tggtctttga
    38401 caactgaata aagcagacag atacgtttgt gagatagcaa gccacgggga ctgtacgcca
    38461 cgaccttttc aaaagaaagc gagcgaccca cgcagtgatc cgagagcgac cgttggaaaa
    38521 gtcgctttgc cacgacctat cctcacagaa taatgatacg tccgcatggt gcggttctgc
    38581 ccacaggaat gggaatagtt gagatactaa cggagctttc caaagccgtc tgcctgcttg
    38641 taaaaaatga cacacagtaa agggggtgca tagattatga acaatactga tgtgcctatc
    38701 tgggaaaaat atacgctgac gattgaagaa gcgtccaaat acttccgcat tggcgaaaag
    38761 aaattgcgta aattagccga agaaaatctt gacgctggct gggttatcgt gaatggtaat
    38821 cgtattcaga ttaagcgaaa acaatttgaa aaaatcatag atacattgga cgaaatctaa
    38881 tgtcattgag ccgtagatat ggtataatat tagtagcgta tcacggctca ttccatgacg
    38941 gaaaggagct ttacactatg tctaatgtga aacgaaaaga cagtaaaaat cgcaatttgc
    39001 gtaatggaga gagccagcga aaagacggaa gatacgttta taaatatacc gatatatacg
    39061 gaaagccaca atttatctat tcttggaaac ttgtaccgac agacaagaca cctgctggaa
    39121 agcgtgatga tatatcgttg agggaaaaag aagcacagat aaaaaaagac cttaacgacg
    39181 gtatcgacac agtcggcggt aaaatgacag tctgccagct ctacgacaag aagaacagcc
    39241 aaagaaagaa catcaaaagg gctactgaaa aaggacgaca gtatcttatg aacgctctga
    39301 aaaatgaccc attgggtatg agggcgattg atacggttaa gcagtcggac gctaaagaat
    39361 gggctatcag aatgagcgaa aaaggatatg cctataaaac gattgataac tataagcgtt
    39421 ccttgaaagc gtcattttac atggcgatac aagacgactg tatcagaaag aacccatttg
    39481 aatttaagtt aagtgatgtt ctggaagatg ataccgaaca gaaagttatc cttacaccag
    39541 agcaggaagg acgcctgctt gcctttatgg aaaaggacaa gatttacagt aagtattatg
    39601 atgaggttgt gcttctgctg gaaacgggac ttcgtatttc tgaattttgc ggactgacga
    39661 cgcatattga tatgcagaac agaatactca atatagacca ccagttattg aaagatagcg
    39721 aaatgggcta ctatattgaa acgccaaaga ccaaaaacgg aaaacgggaa cttccattga
    39781 cagaacgggc ttatcaagca atccaaagaa tactaaagaa cagaggaaag gcacaaccgc
    39841 tgattgtagg tggttacagc aatttcctat tcttaaaccg tgagggtttg cctaaagttg
    39901 caggaaacta tgagggcatg gtgcgagggc tgattaagaa gtataacaag tatcacacgg
    39961 acaagctacc gaacatcacg ccacattcat tccgacatac ttattgtacg aatatggcaa
    40021 acagaggaat gaacccaaat actctgcaat atctcatggg acacgctaac ataaccatga
    40081 cacttggcta ttacgcacac ggcacatttc aatctgcaaa agcagaactg gaaagactgg
    40141 cttgttaata tcggagctta tatttactac tcatttacta cttttggttg cattttcatg
    40201 ctggtaaatg ccagcttatg caaggtatct tccaaaaaga aaataccgat aaagccct
//
LOCUS       DAFKMX010000012        39533 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFKMX010000012 DAFKMX010000000
VERSION     DAFKMX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.012"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            67..834
                     /locus_tag="LZB08_000513"
     CDS             67..834
                     /locus_tag="LZB08_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBM7314032.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1052..1265
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1519..2553
                     /locus_tag="LZB08_000514"
     CDS             1519..2553
                     /locus_tag="LZB08_000514"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBM7314033.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2594..3811
                     /gene="argJ"
                     /locus_tag="LZB08_000515"
     CDS             2594..3811
                     /gene="argJ"
                     /locus_tag="LZB08_000515"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBM7314034.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3826..4686
                     /gene="argB"
                     /locus_tag="LZB08_000516"
     CDS             3826..4686
                     /gene="argB"
                     /locus_tag="LZB08_000516"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBM7314035.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4716..5903
                     /locus_tag="LZB08_000517"
     CDS             4716..5903
                     /locus_tag="LZB08_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBM7314036.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLINKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6012..7034
                     /gene="argF"
                     /locus_tag="LZB08_000518"
     CDS             6012..7034
                     /gene="argF"
                     /locus_tag="LZB08_000518"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBM7314037.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            7161..7778
                     /locus_tag="LZB08_000519"
     CDS             7161..7778
                     /locus_tag="LZB08_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBM7314038.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSIKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8132..9325)
                     /locus_tag="LZB08_000520"
     CDS             complement(8132..9325)
                     /locus_tag="LZB08_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7314039.1"
                     /translation="MSEKRRDNKNRILRTGESQRKDGRYAYKYIDTFGKPQFVYSWKL
                     VPTDKTPAGKRDDIALREKEKEIQKDLDDGIDHIGKKMTVCQLYAKQIRHRANVRHGT
                     KQGRKQLMRILQEDKLGACRIENVKLSDAKEWALRMKEKGYGFKTINNHKRSLKAAFY
                     TAIQDDCIRKNPFDFQLNTVLEDDTEPKEPLSPTQEAAFLSFVQHDKVYQKYYDEIII
                     LLGTGLRISELCGLTEADIDLDKQLINVDHQLLKIADVGYYVETPKTKSGNRVIPMSE
                     KVLEAFQRVLNKRKYAQPVILEGYTKFLFLNRNGLPKVAVNYESMFRGLVRKYTKTQK
                     VALPKVMTPHTLRHTFCTTLANAGMNPKALQYIMGHSNINMTLNYYAHTTSETSITEM
                     KRLIA"
     gene            complement(9405..9608)
                     /locus_tag="LZB08_000521"
     CDS             complement(9405..9608)
                     /locus_tag="LZB08_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HBM7314040.1"
                     /translation="MNNNDVPIWEKYTLTIEEAAKYFRIGESKLRKLAEENPVPDWVM
                     MNGNRIQIKRKQFEKMIDTVDAI"
     gene            complement(10029..10286)
                     /locus_tag="LZB08_000522"
     CDS             complement(10029..10286)
                     /locus_tag="LZB08_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314041.1"
                     /translation="MNGLNGKPTEYPENALVPYPVILAASKGDPDAMKMVLQHFSGYI
                     ASLSMRKLYDERGNVYYGVDEDIRERLQARLMRAILTFRAE"
     gene            complement(10267..10707)
                     /locus_tag="LZB08_000523"
     CDS             complement(10267..10707)
                     /locus_tag="LZB08_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7314042.1"
                     /translation="MTPNRREFEKQCAFQKFCKSVLHNEACNAHEEIRRRRVREVTFS
                     NLALHEERQLYTVDKYFQDEEAEPSYQMAGKEITPKLLLEAIRALPEERRKIVLLYYF
                     EGLTDIEIGKQLNISRSTVQYRRTSSFEQLKKYLEENADEWIEW"
     gene            complement(11170..11790)
                     /locus_tag="LZB08_000524"
     CDS             complement(11170..11790)
                     /locus_tag="LZB08_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBM7314043.1"
                     /translation="MLQKIYPRSKDKETVYLKSVITNPNIIVGDYTMYNDFVKEPKDF
                     EKNNVLYQYPINQDKLIIGKFCSIACGAKFIFNSANHTLSSLSTYPFPIFFEEWGLDV
                     KDITKAWDNKGDIVVGNDVWIGYEAVILAGVTIGDGAIIGARAVVTKDVPPYTIVGGV
                     PAKPIRKRFNEETIDILLKLKWWDWSKERIAKNIAAIQSGNVKQLN"
     gene            complement(11802..12512)
                     /locus_tag="LZB08_000525"
     CDS             complement(11802..12512)
                     /locus_tag="LZB08_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314044.1"
                     /translation="MCTRFVYNGNDMITGFNFDIDLAVWKHKVIKEKERFYIGILRPD
                     GTYHSYHGINRNGNVGTLLYVHDNPAGIYRDNSDCITIADLTEQFIKAQISFDEVLQI
                     VKAKQVTYAPDATMQAMLSDSHGRVLIIEPGIGYKEEHEQYSLITNYSLMKPESTKDF
                     IVPGDDRYERALKKLEKYSPDFSISDAIHLLYDVRQEGAWATRVSFVYSTKEQTVYYV
                     ENNHFECIQTFTFPLERY"
     gene            complement(12646..12909)
                     /locus_tag="LZB08_000526"
     CDS             complement(12646..12909)
                     /locus_tag="LZB08_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314045.1"
                     /translation="MKYKNAQAILPDALVKELQSYVQGEYIYIPVEQEQQKRWGEVSG
                     YRKELEQRNQKIKQEYQSGISINCLAEKYCLSLYAVRKIIYQK"
     gene            13214..13579
                     /locus_tag="LZB08_000527"
     CDS             13214..13579
                     /locus_tag="LZB08_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314046.1"
                     /translation="MIMKQNKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYI
                     ASIENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLK
                     IMSATAKGIEEANNDRTGE"
     gene            13822..14151
                     /gene="mobC"
                     /locus_tag="LZB08_000528"
     CDS             13822..14151
                     /gene="mobC"
                     /locus_tag="LZB08_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBM7314047.1"
                     /translation="MNGRKRKVQIKFYVTEEERALIEQKMKLVPTRNMAAYLRKIAID
                     GYIIQTDHSDIKAMTTEIQKIGVNINQIAKRVNATGSVYQEDIGEIKGVLAEIWRLQR
                     LSLLKAR"
     gene            14112..15458
                     /locus_tag="LZB08_000529"
     CDS             14112..15458
                     /locus_tag="LZB08_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBM7314048.1"
                     /translation="MAVTKIKPVKSTLSKALDYIQNPDKTDGKTLVSSFGCSYETADI
                     EFGYTLSQARQKGNNLAFHLIQSFEPGEVDCEKAHAIGKQLADAVTKGQHEYVLTTHI
                     DKGHIHNHIIFCAVNFVDYHKYNSNKRSYYGIRNMSDKLCRENGLSVVVPGKGSKGKS
                     YAEYQAEKAGTSWKGKLKIAVDTLIPQVASFEELLQRLQAAGYEIKPGKYISCRAPGQ
                     ERFTRLKTLGADYTEEAIKERIAGKRRKTAKVPKEQRGVSLLIDIENSIKAAQSRGYE
                     QWAKIHNLKLAAKTMNFLTEHQIEQYADLVSRIEEINAENEKTAEALKSVEKRLADMA
                     VLMKHVATYQKTKPVYEAYRKAKDKDTYRAKQESNLILHEAAVKTLKAAGVTKRPNLA
                     AIQEEYGKLQAQKEALYADYGKLKKQVKEYDVIKQNIDSILRQPREPEREKGKERE"
     gene            15638..16048
                     /locus_tag="LZB08_000530"
     CDS             15638..16048
                     /locus_tag="LZB08_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314049.1"
                     /translation="MNIADRIQYLRKQNGYSQEELADKVGVSRQAVSKWESEQSTPDL
                     EKVIIMSELFEVTTDYILKGIEPVSTTNRKTIRTLYVGFALIFAIIAGIWSFAANRFN
                     YDEIILITLAGGVVGFGIALIVQVIGGMISNKKQ"
     gene            16104..16760
                     /locus_tag="LZB08_000531"
     CDS             16104..16760
                     /locus_tag="LZB08_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314050.1"
                     /translation="MNKFLNDIRSAENPISSNRKIINTIAVLFLGIALGTFSKFLDFR
                     QVELPSVLMAIDGALDVHNFLGRFAIWVLIALCISIYSNSAIRASVNVFAFFVGMVTS
                     YYLYSNYVAGFFPRSYAMIWFGFTIISPFLAFVCWYAKGKGKLAFMLSILILAVLFNM
                     TFVYGWGYFEALSVLELAVFIIGLTVLRRDTLRSSVLMGTISIVLAFLLDMVIPFHFG
                     "
     gene            16800..17030
                     /locus_tag="LZB08_000532"
     CDS             16800..17030
                     /locus_tag="LZB08_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314051.1"
                     /translation="MEQEMPDYETIRAAVTGEKWALEKVLDCYGDEINHLAMVKKRQP
                     DGTTKEEIDEDLRQTLILKLLEAIPQFPIERE"
     gene            17035..17403
                     /locus_tag="LZB08_000533"
     CDS             17035..17403
                     /locus_tag="LZB08_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314052.1"
                     /translation="MTRGEIWKDFFKRTVLPAVIALFLFFMFKNVFTEDGQTNYFYVW
                     LCCGIPFGIRRMFVWLVPHGYDIAGTVGIVALNFILGGIIGGVILIWRLVVATWYIPL
                     TIYRLLTADKGATPQINMEK"
     gene            complement(17581..17946)
                     /locus_tag="LZB08_000534"
     CDS             complement(17581..17946)
                     /locus_tag="LZB08_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBM7314053.1"
                     /translation="MTGVKRLTPPQSRKVNSLVKKECCNCERGHCILLDDGEECVCPQ
                     LISYSLLCKWFQIAVLPLDKLLYAELFKTEDKKRCSECGTFFVSKSNSVKYCPDCRKR
                     ITRRQAAERMRKRRAAVTQ"
     gene            complement(17943..18149)
                     /locus_tag="LZB08_000535"
     CDS             complement(17943..18149)
                     /locus_tag="LZB08_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBM7314054.1"
                     /translation="MIRLTVEEMNLLSIYHEGSKVQLIENMTAALPFMDEDMRPFAER
                     TLQKISPLTENEYAELAIFAADEV"
     gene            complement(18152..22228)
                     /locus_tag="LZB08_000536"
     CDS             complement(18152..22228)
                     /locus_tag="LZB08_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7314055.1"
                     /translation="MASLRDTVKDYQEELRDGIAWVAFWKMGRSWNAEYFHLEMGDYL
                     YPEDRSRMEEIKQADPAAVVVNGYYSGYLGEDRNLDELTAGVRRHYENGYSNIGEFIE
                     AHDDRLPPELIEEARDAAHAAGLPFSEKAYRDGEEPDPYLFDGSMSMEDYELMHRMIE
                     NERSERMEETILSGYLSNLGKYTEGRPAGEWVSFPTTAEHLKEIFDRIGIDGKNYGEL
                     HITEYQSSIAGLAGKLTELESLDELNYLGELLKMQFDDDREKFVAAMAYGDHTRNLQD
                     IINLAQNLDCYWLYPSVQSEEDYGHYLIEELDELELPEEAKKYFMYEEYGRDAAINDG
                     GRFTEQGYIYNNRNTFTQWYDGRDVPEEYRVTPQPPVQEKEQADLDAAAVQPAFATEQ
                     PPVLPIILSSEKPADKMKEITDRLEQGILGIYESDRYADYLRTMSKFHNYSFNNTILI
                     AMQGGNLVKGYKQWEKEFDRHVKPGEKAIKILAPSPFTVKKQVEKIDPDTQKPVFDKD
                     GKPVTEEKEVKIPAFRVVSVFDISQTEGKELPALTYELTGNVEQYKDFFAALEKTSPF
                     VMGFETLSGGVKGRCNYEEKRIIINEGMDELQNIKTAIHEIAHATLHDTALAMPERPD
                     RRTREVQAESVAYAVCQHYGLDTSDYSFGYIAGWSSGKELAELKGSLETIRSTAAKLI
                     DTIDGHFAEIQKAQDKEQTTEQAQPAQEAAKQPEAEAAAPEVPEEIAPAQEKEAQTEP
                     EADTGASSEAPQPPQPEQATPAAPYYPINEAAAKRAKDMNSFSDYKQGSATAEYRHYV
                     DEAVQLAERQKQRVDPMYHDKIDSLLDTYARKLAANMNKGYEIDARVPSILIAGGSNF
                     PTRKKEKQNAARDSNYREWQDIQGLLDKIRSTGMGGISADDPQAVQKLEKKLESLEKS
                     QETMKAVNAYYRKHKTLDGCPHLSPEQLEKLKADMASSWHLGDKPFATWALSNNSAEI
                     RRVKDRIKSLSQQKEIGFVGWEFDGGKVDANTEANRLQIFFEDKPDETTREALKSNGF
                     RWSPKAGAWQRQLTSNAYYAADYIKAIALLTGEKPTELQRAHIRAQKVAALEQPAQGQ
                     PEQEAPQDKDTFSIYQIKGGDETRDLRFEPYDRLTAAGYRVDVKNYALVYSAPLTPGT
                     SLEDIYTRFNIDHPKDFKGHSLSVSDVVVLHQNGQDTAHYVDSFGYKEVPEFFKEQEK
                     ALIPDELETGETIKTPRGTFYVTAMSRAQMEAAGYGLHHQSDDGKYLIMGNGTRAFAI
                     AAEQPENYLKTAEQTTEQNYNMIDGQINNTPTAAELEEKAKAGGQISLAEYAEALKAE
                     KKQTEPEKKPSIRAQLRADKEKTAQKKAAKTKNHELEV"
     gene            complement(22470..24560)
                     /locus_tag="LZB08_000537"
     CDS             complement(22470..24560)
                     /locus_tag="LZB08_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBM7314056.1"
                     /translation="MILVIAEKPSVAQTIAAVLGAKEKKDGFLTGSGYIISWCVGHLV
                     GLSEAAAYGEQYKKWSYDSLPILPQEWKYTVSADKEKQFKTLKELMNRADVSEVVNAC
                     DAGREGELIFRFVYEMAGCKKPMRRLWISSMEDSAIKEGFSRLKNGEEYDALFASALC
                     RAKADWLIGINATRLFSVLYNHTLNVGRVQTPTLKMLVDRDAAITTFKKEKYYHVRLT
                     LSDAEAASEKLSDRSEADRLKAACEASKAVCTSLVKEKKTVAPPKLFDLTSLQREANR
                     LFGYTAKQTLDLAQALYEKRLLTYPRTDSSYLTDDMGDTAAGIIKLLCGKFPFMAGKD
                     FTPELGKVLNSKKVSDHHAIIPTMELAKTDLAALPESEKNILTLAGARLLMATAAPHT
                     FEAVTAIFECAGQSFTARGKTVLSDGWKEIDRKYRAALKNKPEADNADSDTEKTLPPF
                     TEGQTFENPAATVTEHDTTPPKPHNEASLLSAMERAGSEDTDPDAERKGLGTPATRAA
                     VIEKLVKGGFVERKGKQLLPTKDGINLVCVLPDTLTSPQLTAEWENNLTQIAKGKADP
                     AVFMEGIEDMARELVKTYPFLSDDKSQMFKPEREALGSCPRCGSPVYEGKKNYYCSNK
                     ECIFTMWKNDRFFEERKVAFTPKIAAALLKSGKVNVKKLYSPKTGKTYDGTIVLADTG
                     GKYVNYRVELPKKK"
     gene            complement(24675..24923)
                     /locus_tag="LZB08_000538"
     CDS             complement(24675..24923)
                     /locus_tag="LZB08_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBM7314057.1"
                     /translation="MEDKKILLDNIDKIHTTEMGVDRIKRNLKIDTADVVEYCKNKVL
                     DKNCNIYKQGKNWYCEVENIKITINSYSYTIITAHIVK"
     gene            complement(25198..25923)
                     /locus_tag="LZB08_000539"
     CDS             complement(25198..25923)
                     /locus_tag="LZB08_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBM7314058.1"
                     /translation="MKNKLKKTITALCAALILMGGFSVPAYAQGTAAEPPAEDATNDS
                     NVVVEEPETPPLTPKGNATLVDDFGGNKQLITVTTKGGNYFYILIDRAAEEEQTVHFL
                     NQVDEADLMALTETGEATKKPEVCNCTEKCEAGKVNTSCPVCATTLTGCTGKEPTPTP
                     TEQPEEPKEKGGNPGAVLALVLFVLLGGGAAFYYFKFIKPKQNVKGNTDLEDFDFEDY
                     DEDEPEADTGEVSKEEETEEEAL"
     gene            complement(25913..26185)
                     /locus_tag="LZB08_000540"
     CDS             complement(25913..26185)
                     /locus_tag="LZB08_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBM7314059.1"
                     /translation="MANNKIDRINKEIAKTREKITEYQNKLKGLEAQKTEAENLEIVQ
                     LVRAMRMTPQELNAMLSGGGIPGMSAAPASDEAADYEEQEDNADEE"
     gene            complement(26217..28121)
                     /locus_tag="LZB08_000541"
     CDS             complement(26217..28121)
                     /locus_tag="LZB08_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBM7314060.1"
                     /translation="MTRDGAVEINETQQTAERISSREADSDFSQPDSAAAERVMEHLD
                     AAHTRKASKKAVKKAQEATVLRTSTSRLQFTDEERETPKLQPYIKKSEKAADQLDAAK
                     AAIPKQKKLVKERTFEEATGKAKTRLHFEEQEKPISGGKAHSNPLSRPAQEAGIFVHN
                     KIHSVEKDNSGVEGAHKSEELAERGARYGTRKFKQGYRSHKLKPYREAAKAEQAAFKA
                     NVNFQYHKALQENPQLTSNPFSRFMQKQKIKCQYAKTVKKGGAATAKAAAGASQTAAK
                     KATAFAGRHPAGVIIAIAALLLFIMVSVGLSSCGAMFSGSMNSVLGTSYTSEDSDLVA
                     TEQSYAAMENELQQEIDNIESTHSGYDEYRYDLDTIGHNPHELASYLTALLQSYTPQS
                     AQAELKRLFGLQYTLTLTEEIEIRTTTDEEGNEEEYEYRILNVKLTNKPIASLAEELL
                     NPQQFEMFKVYLQTQGNKPLIFGGGSPDGSPSEDLSGVEFVNGTRPGNPELMELAKQQ
                     VGNVGGYPYWSWYGFNSRVEWCACFVSWCYNQAGKSEPRFAGCEWQGVPWFQSRGQWG
                     ARGYENIAPGDAIFFDWDLDGVADHVGLVLGRDGSRVYTVEGNSGDACKIKSYDLNYQ
                     CIKGYGLMNW"
     gene            complement(28157..30586)
                     /locus_tag="LZB08_000542"
     CDS             complement(28157..30586)
                     /locus_tag="LZB08_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314061.1"
                     /translation="MSKGQTKKTRETAGNRRKLTRAEKKQIAAVIRQAKGDGKAHTAQ
                     QTIPYLAMYPDGICKMAQRRYSKSIAFEDINYQLAQADDKTAIFENWCDFLNYFDASV
                     NVQLSFINQGSQQEQAARAIHIPPQNDDFNSIRTEYSDMLKTQLAKGNNGLVKHKYIT
                     FSIEADNPAAARARLSRIETDILNNFKVLGVSAHPLSGYERLKVLHGVFHPAGEPFSF
                     SYDWLTPTGLTTKDFIAPSSFKFGEGRYFAMGKKTGAVSFLEILAPELNDRILADMLD
                     LETGVIVNLHIRSIDQAEAIKTIKRKITDLDKMKIEEQKKAVRSGYDMDIIPSDLATF
                     GNEAKNLLQDLQSRNERMFLLTFLVVNMADTKRKLENDIFAAAGIAQKYNCALTRLDY
                     QQEAGLMSSIPLGENLIPIQRGLTTSSTAIFIPFITQELFQTGAALYYGLNALSNNMI
                     LCDRKQLKNPNGLILGTPGSGKSFAAKREITNAFLITDDDIFICDPEAEYFALVKRLG
                     GQVIRLSPTGKGMDGTPQYVNPMDMNLNYSEDDSPLALKSDFILSLCELVIGGKEGLQ
                     PVDKTVIDRAVRNVYQDYLADPVPEKMPILGDLYNELLKQPEPEAARIAAALELYVSG
                     SLNVFNHRTNVELSNRLVCFDIKQLGKQLKKLGMLIVQDQIWNRVTINRAEKKSTRYY
                     MDEFHLLLKEEQTAAYSVEIWKRFRKWGGIPTAITQNIKDLLASREVENIFENSDFVL
                     MLNQAQGDRAILAKQLNISPQQMKYVTHTEAGEGLIFYGNVVLPFVDHFPKDTELYRI
                     MTTKPEEVSSL"
     gene            complement(30534..30938)
                     /locus_tag="LZB08_000543"
     CDS             complement(30534..30938)
                     /locus_tag="LZB08_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBM7314062.1"
                     /translation="MAYVPVPKDLSKVKTKVAFNLTKRQILCFSMALLMGLPLFFLLK
                     DSAGTSLAAMAMIVVMLPCFLVAMYEKHGQPLEVVVKNIIQTKFTRPKVRPYQTENLY
                     ALLEKQRALEKEVSAIVKRTDKKDAGNRRKQA"
     gene            complement(30981..31850)
                     /locus_tag="LZB08_000544"
     CDS             complement(30981..31850)
                     /locus_tag="LZB08_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314063.1"
                     /translation="MESILEAINEWIKEILIGAINGNLSTMFGDVNEKVGTIAAEVGK
                     TPQGWNANIFNMIQNLSENVIVPIAGLVITYVLCYELISMVTEKNNMHDIETFMFFKW
                     FFKAFVAVFLVTHTFDITMAVFDMAQHIVSGAAGVIGGDTNIDVTEALAAMQEGLKDM
                     EIPELLLLVMETSLVSLCMKIMSVLITVILYGRMIEIYAYCSVSPIPFATMTNREWGQ
                     IGNNYLKGLFALGFQGFLIMICVGIYAVLVGEMVLADNLHSAIFSLAAYTVILCFSLF
                     KSGALAKSIFNAH"
     gene            complement(31869..32084)
                     /locus_tag="LZB08_000545"
     CDS             complement(31869..32084)
                     /locus_tag="LZB08_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBM7314064.1"
                     /translation="MEFFNSAIDVLQTLVIALGAGLGIWGVINLLEGYGNDNPGAKSQ
                     GIKQLMAGGGVALIGTILVPLLSGLFG"
     gene            complement(32296..32466)
                     /locus_tag="LZB08_000546"
     CDS             complement(32296..32466)
                     /locus_tag="LZB08_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314065.1"
                     /translation="MNRRIRKKKDKKITEAINGLISIAEWQERQWQAAIQQFVKRCEA
                     LYEQQRKEELTQ"
     gene            complement(32459..34249)
                     /locus_tag="LZB08_000547"
     CDS             complement(32459..34249)
                     /locus_tag="LZB08_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBM7314066.1"
                     /translation="MNAINWKKLLLPNIPYLLFVYLFDKVGQAVRLAPGADLSGKVLS
                     LGEGFSMAFSNPLPSLAPMDLLIGILGAVLIRLIVYVKGKNAKKYRKGIEYGSARWGT
                     AEDIKPYTDPVFQNNVLLTQTERLTMNSRPKQPKYARNKNILVIGGSGSGKTRFFVKP
                     NLMQMHSSYVVTDPKGTVLVECGKLLQRGGYRIKVLNTINFKKSMKYNPFAYLRSEKD
                     ILKLVNTLIANTKGDGEKAGEDFWVKSERLFYCALIGYIWYEAPEEEKNFTTLLEMIN
                     ASEAREDDPEFQSPVDLMFERLEEKDPEHFAVRQYKKFLLSAGKTRSSILISCGARLA
                     PFDIKELRDLMETDEMELDTIGDRKTALFVIISDTDDTFNFVVSILYTQLFNLLCDKA
                     DDEYGGRLPVHVRCLLDEFANIGQIPKFEKLIATIRSREISASIILQSQSQLKAIYKD
                     NADTIVGNCDTTLFLGGKEKTTLKEMSEILGKETIDSFNTSENRGREVSHGLNYQKLG
                     KQLMTEDEIAVMDGGKCILQLRGVRPFFSDKYDITKHPNYKYLSDYDKKNTFDMEKHL
                     RRRPALVKSDEPFDYYEISEADLQEDTAHE"
     gene            complement(34246..34728)
                     /locus_tag="LZB08_000548"
     CDS             complement(34246..34728)
                     /locus_tag="LZB08_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBM7314067.1"
                     /translation="MQEETNEKTIALYIKTGKLTAQQLQKAMKALLAQMKKQHDRQKI
                     PHGKQTLKQLMKQNAGVSNIEITKDNIKAFESTAKKYGIDFALKKDSTEIPPRYLVFF
                     KGRDADALTAAFKEFSAKKLTQEQKPSIRKLIVSLKEKAAALNAQRDKVKNKDRGIAR
                     "
     gene            complement(34822..35040)
                     /locus_tag="LZB08_000549"
     CDS             complement(34822..35040)
                     /locus_tag="LZB08_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBM7314068.1"
                     /translation="MTQKTGALIFDETADRYDIRFDVNDYYGGLHCGDCMEVFVRGKW
                     KPTRMEYGDNWYLVGIRAADLSGLRVRI"
     gene            complement(35065..36039)
                     /locus_tag="LZB08_000550"
     CDS             complement(35065..36039)
                     /locus_tag="LZB08_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314069.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSLINRESIDAIRTAVWELEKAGYITRRQGRDEKGKMTAIEYTIYEQPQPPAL
                     DCPVLENPTADKPILENPTPDNPTSENPTQLNKELLKTNLPKKEKLNTDVSSTHSIPF
                     HSLNPSPLEDAAQPPERKRKEATDAYSVYEEIIKDNIEYDYLIQDRYLDRDRIEEILA
                     LILETVCTKRKTIRIAGDDYPAELVKARFMKLNSEHIRFVLDCMQENTTKIRNIKQYM
                     KAALFNAPSTIGSYYTSLVSHDMADGTFYGKPKKSGIPDYTCNEGESL"
     gene            complement(36482..37180)
                     /locus_tag="LZB08_000551"
     CDS             complement(36482..37180)
                     /locus_tag="LZB08_000551"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBM7314070.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(37342..38559)
                     /locus_tag="LZB08_000552"
     CDS             complement(37342..38559)
                     /locus_tag="LZB08_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBM7314071.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(38750..39163)
                     /locus_tag="LZB08_000553"
     CDS             complement(38750..39163)
                     /locus_tag="LZB08_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7314072.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT        11038 a         7942 c         8433 g        12120 t
ORIGIN      
        1 aatattaaga taaaaatata tagaatatat ttaaaaatta ttatgcaatt agaaaaggag
       61 ataagtatgc agattattga tataaatata aataatattg acaatgaaca tatatgttgt
      121 tcaatttctg aaaaaaaagg agaaaattgt gttttttcta aaaaagcttg gatgaaaaat
      181 aattttgata aaggtctagt ctttaaacga tttgatgtaa gaggtaaagc ttttatagaa
      241 tatattccag cagaaaatgc atggtgtcct attactgcgg acaattatat atttattaac
      301 tgtttttgga tatctggaaa atttaagggt caaggatatt ctaatctatt gctacaagag
      361 tgtgttgatg atgctaaatc aaaaggtaaa tatggattag ttgtgctttc gtctagtaaa
      421 aaaatgcctt ttttatctga tgctaaatat ctaaaatata agggcttttg tttagcagat
      481 acatcagaac caaattatga gctattatat tttcctttta ctgataatgc tcctattcca
      541 tgttttaaga attgtgtaaa acaaagaaaa ttaaatgaga atggtatagt tttatattac
      601 agtaatcagt gtccacatac ggaaaaatat gctcctattt tgaaaaaaat tgcaaattct
      661 agaggtattg atgtaaatct tataaaattc gaaacagcaa aagaagcaca aaatgctcct
      721 gcaccattta ctacatacag tctttttttt aatggtgaat ttgtaaccaa cgagatactt
      781 tctgagaaaa agattgagaa gtttttagat accaacatta aaaaatttaa ctaaaaagag
      841 atgtaaatat tgtattttga aaatatatta ttttataatt taatatattt tggatgtttt
      901 ttaaattgac gaatgaaata taaaaattat atcgcaattt agatacaaaa tgctggaaat
      961 taaccatata gaaaatattt ccaaaaattt aaaaattata ttgacaaagt taaaaaaata
     1021 aactatcata taaaacaata agaaaataaa ttgaaatcct gtgaaaaaga gagtagcata
     1081 aagctagttg atagagagct gggaatggtg gaagcctagt aacgaatttt attgtgaata
     1141 gagctttgga ggagattacc agaagtaata gtatggtaat tggtgaatct cgccttaaga
     1201 gattagagtg ggtaagatta tcttatcaat tagagtggta acgcgggtgt atattcgtct
     1261 cttggtcttt gaccaagagg cttttttaat tttaaatgta cattttgtag atacttttta
     1321 atttgagttt taatgattat tgtaatttta ccaataagag tggtaccgcg gaaaatcgcc
     1381 tcttagtatt tataatgcta aggggttttt tgttttaatt agagtatttt aatctacgta
     1441 ttatcaaatg attatcatat agtaaagtgg aaagatagga ttttaagact atttgaatat
     1501 aaaaatggag ggaacaaaat gaataaaata aaagttggta ttattggtgc aactggatat
     1561 gtcggggcag agttgattag attattaatg aatcatgata aagtcgaagt gacagcgata
     1621 ggttcaaatt cgtatgttgg aaaagatata gttgatatat atccaagtat aggatataaa
     1681 aataacatga tatgtataga aaatgaaaaa gttatagata tgtgtgatgt tgtatttaca
     1741 gcccttccac atggagttag tgaaaagttt gtaataaagg caattaaatc gaaaaagaaa
     1801 gttatagatt taggtgctga ctttagaata aaagatgaag aagtatatag taaatggtat
     1861 ggagtatctt ttatagataa aatcttacat aaaaaagctg tatatggatt aagtgaaata
     1921 tacaaagaag atataaaaga tgcagatata attgcaaatc ctggatgtta tccaactagt
     1981 atttctttac cattgatgcc attactaagt tctaaattga taaaaaataa taatataata
     2041 attgattcta aatcaggtct tacaggtgct ggtagagaat tatcgattag tagtcacttt
     2101 acggaggtta atgaaaatat cactgcatat aaaataggga aacacagaca tactccagaa
     2161 atagaacaaa atttaagtga atcctgcaaa gaaaaagtaa gtgtagtttt tacaccaaac
     2221 ttaatcccag taaatagggg aatcttatca actatatatt gtacaaaaga agataatatt
     2281 tctataaatg atatacatag gaaattaact gattattatg aatttaaaga atttatagaa
     2341 attcttccat tagataaggt agcttcttta aagaatgtta gattctcaaa taaatgtgta
     2401 atatcattac atgaaaatgg agatacattg ataatctgtt cagctataga taatatgatt
     2461 aagggagcag caggtcaagc tatacaaaac atgaatataa tatttggtat tgaagaaaat
     2521 acaggtttaa aaaatattgc accatcattt tagaaataaa aaatactaaa ctcatataat
     2581 taggaggata aatatggaga ttataaaggg tggagttaca gtttcagaag gattttttgc
     2641 ttcagggatt cactgtggct tgagaaaaaa taaagaaaaa agagatttag ctttggttta
     2701 ttcagatgtt ttatgtgatg cagcagcagt agtaactcaa aataaagtaa agggcaatcc
     2761 tgtttatgtt acacaggaac acattaaaaa tggaaaagca caagctatca tagtaaatag
     2821 tgctaatgct aataccttta atggaaaaga aggacttata aatgcttata aaatggctaa
     2881 attcaccagt gataaattaa atattaaaga aagtgatgtt ttggttgcat ctacaggtgt
     2941 tataggaaag ccattaaata tagatttaat agaagaaaat atggatgaat tagtgaataa
     3001 tctaagtaaa caaggtcata ttggagcaag agaagctatt atgacaactg atattataaa
     3061 aaaagaaata gcagtagcaa taatgtatgg agataaaaaa ataactattg gaggtatggc
     3121 aaaaggttct ggcatgatac atcctaatat ggcaacaacg ttaggattta taactacaga
     3181 tgcaaatatt gatggagttt tgctaaaaga agctttaaaa attgctgttg acaaaagttt
     3241 taatagagtt agtgtagatg gagatacaag tactaatgat atggtaatga ttctagcaaa
     3301 tggaaaagca aaaaatgata gaataaacaa aaaagatgaa cattatcaag tatttttgag
     3361 tgcacttaca tatgtctgca tagaacttgc taaacttgtt gcaaaagatg gagaaggatg
     3421 tacaaaacta attgaatgct atataaatgg agcattaagt gaagaacatg cagtaaaatt
     3481 agcaaaaaca gttatatctt cttcattagt aaagacagct gtatttggag ctgacgcaaa
     3541 ttggggtaga atactgtgtg ctttaggtta tgcaggtgaa gaaatagata tggaaaaagt
     3601 tgatattatt tttgagagta tgaaaggata tattgaagta tgtaaaaatg gaaatggatt
     3661 agattttaat gaggaaaaag caaaaaaaat ccttgaagat gacgaaataa gtatattagt
     3721 agatttaaat atgggcaatg ccagaggaaa tgcatgggga tgtgatttaa gctatgatta
     3781 tgtaagaata aatggaagtt atagaaatta ggaggatata ctttaatgat taatatagaa
     3841 aaagcaaata cacttataga agctttacca tatatagaaa aacatcaggg aaagactatt
     3901 gtagttaagt atggtggaag tgcaatgaaa aaagatggtc taaaagaatc tgttatggaa
     3961 gaccttgtgt taatgagtta tgttggaatc aatatagttt tagtacatgg tggaggggca
     4021 gaaatcaata agatgctagc aaaagtggat atagaaagta aatttgtaaa tggtcttaga
     4081 tatacagatg aagagaccat ggaaattgta aaaatggtac ttgctggaaa ggttaataaa
     4141 gacttagtaa ataaaataca tacaaaaggt ggaaaagcag tagggctttg tggaatagat
     4201 aataatatga ttttatgtga cccatataaa aattatgaac ttggatttgt aggagaaatc
     4261 aaaaaagtaa atgttgagct tatagaatct tgtctaaagt caggttatat ttcagtaatt
     4321 gcaactatag gtgttggaga tgatggagag acttataata taaatggtga cacagcagct
     4381 tctgctatag ctaaagagtt aaatgcagat aaattgatac ttcttacaga tgtccctggt
     4441 ttgttaagag agccagatga agaaaaatca ttaataacag aagttatatt agaggatgtt
     4501 gataaattat ttgaagaagg tataattact ggaggtatga tacctaaaat agagggatgc
     4561 gtagatgcct taaataacgg agtaaatagg gtacatattt tggatggaag agttccacat
     4621 tctataataa cagagctatt tactgatagt ggaataggta cattaataag aaaagaaaat
     4681 gaataaaatt ataagtttaa gaggggagaa taagaatgaa taacgacaat acaatttcaa
     4741 aatggaatga atattttata gatacatata accaacccaa ttttgtcata gattatggtg
     4801 aaggaagttg tttttttgat actaatggca ataaatatat agattttaca agtggatatg
     4861 gagtatcttc tcttgggtat agtaatagta atttaaagaa tgctctaaaa gagcaagtag
     4921 ataaattatt acatacttca aatttatatt ttaatgagcc agtgttgtat tcaggagaga
     4981 aaataattaa ttcaagtgga atggctaaag tatatttttg taattctgga acagaagcaa
     5041 atgaaacagc attcaaaata gcaagaaagt atagtagtga caaatatggt aatggtagag
     5101 gaactatcat aagtttaaaa gactcttttc atggtagaac aatgatgtct ttaatggcaa
     5161 ctggtatgga caaataccat aagtattttt atcctcttcc cgaaggtttt aaatatatag
     5221 aaagaaataa tattgaagat ttaaaaaata atcttgattc cacagtttgt gcaataattc
     5281 ttgaagcgat tcaaggcgaa ggtggagtta atgttcttga aaaagattat gttttggaaa
     5341 tagtaaaaat atgtcaagaa aaggatattg tagtgatttt tgatgaagtc caatgcggta
     5401 taggaagaac tggtaaactt tttggctatg aatattttga tgtaaaacca gatatagtta
     5461 ccgtagcaaa agggttggga gcaggaatac cagtaggagg agtattgata aataaaaaac
     5521 tttcaaaagt actaggaaaa ggagaccaag gtacaacttt tggaggtaat ttattagcta
     5581 tggtggcagc ttcagttgta ttagatgaga tatctaaaga tggtttttat aatgaagtat
     5641 tagaaaaagg aaattatata agaaaatcta ttgaaagttt taataacaaa gttgtattaa
     5701 agacaaaagg tattggtcta atgataggaa ttgaaactaa tattgaatct agtattatag
     5761 aagagaaagc tagaaaaaaa ggattgctta ttttgactgc tggaaaaaat gtattgagat
     5821 ttttaccacc attaacaatc agttataagg aaatagatga ggctcttgaa atcctaaaag
     5881 atatattact agaaattaac taaatattca aaaaatagtt gattaaagat aaaaattaga
     5941 agtaaataaa aaagtaaagt gctaacaagt ttaattgaat ttaaaaagtt tataagaagg
     6001 ggcgaattaa tatgaataat ataagtttaa aaggtaagag ttttttgaca ttaaaggatt
     6061 ttacaaaaga agagattaga tatttattaa ctttatcaag ttatttaaaa actaagaaaa
     6121 aagttggtat aaaaggcgat ttgctacaag gaaaaaatat agctttatta tttgagaaaa
     6181 catcaacaag aactagatgt tcatttgaag tagcagctca tgatgaagga gcacatgtaa
     6241 cttatctagg tccaaatgat tgtcatatgg gtaaaaaaga gtctgtatca gatacagcaa
     6301 aagttctagg aagattctat gatggaatag aatttagagg ctttaaacaa gaaacagttg
     6361 aaagtctagc aaaatattct ggtgtacctg tttggaatgg acttacagat gaatatcatc
     6421 caacacaaat actggcagat tttttaacag taattgaaaa tgtagataaa gatttgaata
     6481 aagttaaatt tgtatatgtt ggtgatgcta gaaataatat gggaaactca ctgatgatag
     6541 gatgtgctaa aatgggaata catttcgtag cactagcacc taaggaactt tggccaaatg
     6601 aagaattggt taaggaaatg aaggaaatag ccaagaaatc caaaggagat ataatgttga
     6661 ctgaaaatat tgatgatgtt aaaggtgcag atgttatata tacagatgta tgggtttcta
     6721 tgggagaaga ggagcaatat gaagcaagaa taaatcaact taaagattat caagtaaata
     6781 tggatatgat aaagaaaact gaaaatgaaa atgttatatt tttacactgt cttccagcat
     6841 ttcatgattt agaaactaag gttgctcagg agatttatga aaagttcgga ttatcagaat
     6901 tagaggttac aaacgaggta tttaattcaa aatattcaaa ggtatttgaa gaagctgaaa
     6961 atagaatgca tacaataaaa gctgttatgg tttctactat gactgatgta aatatgaaca
     7021 ataatttagt atagatatgt tttaatatag actttacttt aatgaaaatt tataaaatat
     7081 tttaaatata ttattcaaaa actctcatta tatgttaaaa taacttatgt atcaacaaat
     7141 taagagggag gatttgagat atgaaaaaaa tcatattagc tttaacaact tttttattag
     7201 caatatcttt aacagcatgt tcaagtgcaa aaccagaaga tacaattgat agttttttta
     7261 acagtgcgaa aaagtttgac tttgaaggca tgaataaggt aatggaaaat aatgacgaaa
     7321 aatataagga tatattaaag gaattagata caaaagaccc aaatgcccaa tatgttttag
     7381 attatctaaa acagaatgca tctaaaatca catatacaat aaaagacagc gaggttaaag
     7441 gtgataaggc tacaatcaaa gtagaatgta aatttataga ttcaacacca ttacttcaag
     7501 aaatagtagc tgaggcattt acaaagatga ttggtatgag tttttcagga caagacttaa
     7561 ctgatgagaa aacaactgaa atgttagtat caattatgaa agaaaaacaa aaaagcatta
     7621 aagagactta tgttacaaaa aatgtagagt ttgaatgttc taaaaaggat aataaatgga
     7681 taataagttc agtaaatgat gcagtagctg atgtattgtt atctaatctt gttacagcag
     7741 gacaagaatt ttctaactct atgaatacac ttgaataata ttgtttaggt gtagtaaagt
     7801 aataaatata tgattatttt tataaataaa catataaata tttatgaagg ataccatttt
     7861 tggtatcctt tttattaata taatgaaata catggttaat ttctatattg tcaagaaact
     7921 atgatatatg ttataagtct atactattta agataaatca caaagtaatt tttgactatc
     7981 ttttcaaaag ttcttatatg ccggtatagc ctattgttac aggcttttcc ggcatttttc
     8041 gttatggaag atattctcaa atcttcttat atccccgtat gttttggtgt tcaaaagtag
     8101 tatgtggagt agtaaattct ccgtttacta cttatgcaat cagccgcttc atttctgtta
     8161 tcgacgtttc ggaagtcgta tgcgcgtaat agttcagcgt catattgatg ttggaatgtc
     8221 ccatgatgta ctgcaatgcc tttgggttca ttcctgcatt tgctaacgtg gtgcagaatg
     8281 tgtggcgtaa ggtgtgcggt gtcattacct ttggcaatgc taccttttgc gttttggtgt
     8341 atttccttac aagcccccgg aacatacttt catagttgac tgccactttt ggcaagccgt
     8401 tccggttaag aaacagaaac tttgtgtagc cctctaaaat aacaggctgt gcatatttcc
     8461 gtttgttcag cacccgttga aatgcttcca acactttttc actcatagga ataacccgat
     8521 tgccgctttt cgtcttaggc gtttccacat agtaacccac gtctgcaatc ttcaaaagtt
     8581 ggtggtctac attgataagc tgtttgtcta agtcaatgtc agcttccgtc agtccgcaga
     8641 gttccgaaat gcgaagccct gtccctaaca gtatgataat ctcgtcgtag tatttctgat
     8701 agactttatc atgctgcaca aaggacagaa aagccgcttc ctgcgtaggg gatagaggtt
     8761 cctttggttc cgtatcatct tcaaggactg tgttcaactg gaagtcaaat ggatttttac
     8821 gaatacaatc gtcctgtatg gctgtgtaaa aagcagcctt taaggaacgc ttgtggttgt
     8881 tgatagtctt gaagccgtaa cctttttctt tcatgcgtaa tgcccattcc tttgcgtcgg
     8941 agagcttcac attttcaatc cggcaggctc caagtttatc ctcctgcaaa atccgcatga
     9001 gctgttttcg cccctgcttt gtaccatgcc gcacatttgc ccggtggcgt atctgctttg
     9061 cgtagagttg gcagaccgtc attttctttc cgatatggtc gataccgtcg tcaaggtctt
     9121 tttggatttc tttctctttt tccctcaacg ctatatcgtc gcgttttcct gccggggttt
     9181 tgtccgtagg tactaacttc caagaataca caaactgcgg ttttccaaag gtatctatat
     9241 atttgtaggc atatctcccg tcttttctct ggctctctcc ggtgcggaga atacgatttt
     9301 tattatcccg tcttttttcc gacatcgtta tgctcctttc cataatggaa agagccttga
     9361 tatgcttgac tacattgtac cacattcaag gctcgatttc actatcagat tgcgtccacc
     9421 gtatcaatca ttttttcaaa ctgcttacgc ttaatctgaa tacggtttcc gttcatcatt
     9481 acccagtccg ggacgggatt ttcttctgcc agtttccgca gcttgctttc tccgatacgg
     9541 aaatattttg ctgcttcttc tatggtaaga gtgtactttt cccaaatcgg cacgtcgtta
     9601 ttgttcaatt tgtcgtcccc ctttctaaat gggtcaaaag tcaatttgta acataggctt
     9661 taatcggacg gctattagcg taacctcatg ggacttgcac ccctccggcg gtctgccgaa
     9721 gccctaccca ttgcctgcga cgcttctaac gctcggacta tggctataag gaagtatcat
     9781 tatgtattct gtgtgttatc gcccgcaggc tgctatccct gctttacggt gtggtatttc
     9841 tcgctcgctt ttctattaag agaaaggtca tggcgtaccc accgcatggc tcggcacaaa
     9901 aggaacgtat ccgatttgcc ttatgctgcg tcgccgttct attgcgccgc tttctttatc
     9961 aaagagcagg gggctgtctt gcggtatgga acggaaaagg ggaaagaagc gttccaatat
    10021 cgcgtaggtt attcagcccg aaaagtgagg attgccctca tcagtcttgc ttgcagccgt
    10081 tcccgaatat cctcgtcaac gccataatag acattgccgc gctcatcgta gagcttccgc
    10141 atggaaagac ttgctatgta gccgctgaaa tgctgcaaga ccattttcat agcgtccggg
    10201 tcgcctttgc tcgccgccaa aataacggga tagggaacaa gggcattttc cgggtactcg
    10261 gtcggtttac cattcaatcc attcatcagc gttttcctcc aaatattttt ttaactgctc
    10321 aaaggagctt gtccggcgat actgtaccgt acttcttgag atattgagtt gctttccgat
    10381 ttctatatca gtcaatccct cgaagtaata cagcagtaca attttgcgcc gttcttcggg
    10441 taaagcccgg atagcttcaa gcagcagttt tggggttatt tcttttcctg ccatttgata
    10501 agacggctct gcttcttcgt cttgaaaata cttatcgacg gtataaagct gccgttcctc
    10561 atgcaaggca aggttggaaa aagtaacctc ccgaactctg cgccgacgaa tttcttcatg
    10621 ggcgttgcat gcttcattgt gcagcactga tttacagaac ttttgaaacg cacattgctt
    10681 ttcaaattcc ctgcgattag gtgtcatggt gttcacctcc tttctgccga aaggcggtag
    10741 tacctcccct tttcgcggga taatacaggc gactttttgc tttgccaaaa agaaggcaaa
    10801 gtttttgaaa aatcttctgt aaaatgcgaa atgcgcctgc ctgcaaaatg cagacagacg
    10861 cataggaaat aacagcagta tttaattgat ttaacagttc atctacatag ccggaataac
    10921 ccgttgtagg atacaaactt tttttgacaa aagacgtcct tttgtcagat aagcataact
    10981 tattttattc agcataacgg caacctccta tataccgcca tgctgattgc aaggtcaaat
    11041 gaggtcgtcg ttgctaaatt tatgcaaaaa gaagcggcgg ctctgaaatt caaaaccacc
    11101 gctgaaaaaa cgcattaaaa gatgtctgca ttgcgacggc tttttttctt ttgccgtcgc
    11161 gcggcagatt tagtttagtt gtttcacatt accagattga atggcagcaa tatttttagc
    11221 tattctctct ttagaccaat cccaccattt taatttgagc aatatatcaa tcgtttcctc
    11281 attaaatctt tttcgtatag gttttgcagg tacaccgccc acgattgtat aaggtggaac
    11341 atctttcgtt acaacggctc gcgctccgat aatagcaccg tcgccaatcg taacgcccgc
    11401 taaaatgact gcttcataac caatccaaac atcatttccg acaacaatat ctcctttatt
    11461 atcccatgcc tttgtaatgt cttttacgtc taatccccat tcttcaaaaa aaataggaaa
    11521 tgggtatgtt gataaggacg ataatgtatg atttgcacta ttgaaaataa actttgctcc
    11581 acacgcgatt gaacaaaatt tcccaattat caatttgtct tgattgatag ggtattgata
    11641 taaaacatta tttttttcaa agtcttttgg ttctttcaca aaatcattat acatagtata
    11701 atcgcccact atgatattag ggttagtgat aacactttta agatataccg tttctttatc
    11761 ttttgaacgt ggataaattt tctgtaacat aatggttcct cctagtatcg ctctaacgga
    11821 aaagtgaatg tttgaataca ttcaaaatgg ttattttcta cataatatac ggtttgctct
    11881 ttggtagaat acacgaaaga aactctcgtt gcccatgcgc cctcttgacg tacatcataa
    11941 agtaaatgaa ttgcgtctga aattgaaaaa tcggggctat atttttctaa ttttttcaaa
    12001 gcacgttcat atctatcgtc ccccggaaca ataaaatctt ttgtactttc cggcttcatc
    12061 aaagaataat tcgttatcaa ggaatattgt tcgtgttctt ctttatagcc tattcccggt
    12121 tcaatgatta aaacacgtcc atgactgtct gataacattg cctgcatggt tgcgtccggt
    12181 gcataggtaa cttgctttgc ctttacaatt tggagtacct catcaaaaga aatttgtgct
    12241 ttaataaact gttctgtcaa gtcggcaatc gttatacagt cagaattatc tcgatatatt
    12301 ccggcaggat tgtcatgcac atataacaat gtcccaacat ttccattcct attgatacca
    12361 tgataagagt ggtatgtacc gtcgggtcgt aaaataccta tataaaagcg ttctttttct
    12421 tttatcactt tatgtttcca cactgcaagg tcaatatcaa aattaaatcc tgtaatcatg
    12481 tcattcccat tataaacaaa tcgggtacac ataatagttc cctcccatgt attttaaata
    12541 tttcttcttt atcatacagt tcgttgaaaa atttttctat actctatata ataccgaacc
    12601 aatctgtata aaaataagag gctgcacact cggcagcccc ttaccttact tttgatatat
    12661 aattttccga acagcatata aagatagaca atatttttcg gcaagacaat ttatagaaat
    12721 accgctttga tattcctgtt ttatcttttg attacgttgc tctaattctt tcctgtaacc
    12781 ggaaacttct ccccaacgct tttgttgttc ctgttcgaca ggaatataaa tatattctcc
    12841 ttgaacataa ctttgtaact cttttaccaa tgcgtccgga agaatagctt gtgcattttt
    12901 atatttcatg caggcttcct ccttgattat gatagtcaag ttacaaagcc agcatttatt
    12961 ctgcgactat tttactccat gcaaccgtcg caatctactg gctttgcatg gagaaaatct
    13021 ttgtttctct ttttggtgtt gatacacata taaatcactt ctttcaatca aatagggatt
    13081 attgtcagta taacattttc atctcaaaaa gacaacgctg cttttaatat aatatgctct
    13141 atttctatcc atactatgat atgtgtaatc atatctcaaa gaaaatagga acagtaaaat
    13201 gtaactgggt gaaatgatta tgaagcagaa taaagagaaa ttcgatttta aggctttcgg
    13261 gcaagccatt aaagcagcaa gaaaagcaaa gggaatatca cgcaatcagt tagccgacac
    13321 gctgaatatt gcgccccgat atatcgcgtc cattgagaac agcgggcagc acccaagttt
    13381 acagatttta tatgaacttg taaccctgct tgatgtatca gtagaccagt tctttttccc
    13441 agagcgggaa caggagaagt ccacacgccg ccgtcagctc gataccatgc ttgacacaat
    13501 gagcgaaaca gacttaaaaa tcatgtctgc tactgcaaaa ggcatagagg aagccaataa
    13561 tgacaggacg ggggaataaa gttccctcgt ttttgtctgc ataaagtgat tgaagcgtcg
    13621 caggattgcg gcgttttctt ttgcgctttt ttggcatact gggaatttcg ttgtagtggt
    13681 aagtgaggaa aaaactttct tagcaagcat aggaaaagag tacccttttc cgtattttcg
    13741 tcctcccgcc ctgtggtgcg tcggttgaaa attgcttgtt gggaagtgtc ccaaaccctc
    13801 ggaacagaaa ggagtgattg catgaacgga cgcaaaagaa aagtacaaat caaattctat
    13861 gtaacagagg aagaaagggc attgattgag cagaaaatga agctcgtccc cacccggaac
    13921 atggcggcgt atcttcgcaa gattgccatt gacgggtata tcattcagac agaccatagc
    13981 gatataaaag ccatgacaac ggagatacaa aaaatcgggg tcaatatcaa tcagattgca
    14041 aagcgtgtca atgcaacagg cagcgtctac caagaggaca taggggaaat aaagggggtg
    14101 cttgcggaga tatggcggtt acaaagatta agcctgttaa aagcacgttg agtaaagccc
    14161 ttgactatat ccaaaacccg gataagacag acgggaaaac gcttgtgtcc tcctttgggt
    14221 gcagctatga aacggcagat attgagtttg gatatacctt gtcgcaggct cggcaaaaag
    14281 gaaataactt agcctttcat ctgatacaat cgtttgaacc gggagaggta gactgtgaga
    14341 aagcccatgc aatcgggaag cagcttgccg acgcagtaac aaagggacag cacgaatatg
    14401 tcctaaccac ccacatagac aaggggcata tccataacca tattattttt tgcgctgtca
    14461 atttcgttga ctaccataag tacaattcca ataagcgcag ctactacggc atacggaaca
    14521 tgagcgataa actgtgccgg gaaaacgggt tatccgttgt cgtaccggga aaaggcagta
    14581 agggaaaaag ttatgcggaa tatcaagctg aaaaagccgg gacaagttgg aaaggcaagc
    14641 tgaaaattgc cgttgatacg cttatccccc aagtggcaag ttttgaagaa ttgttgcagc
    14701 ggttgcaggc ggcgggctat gagataaagc cgggaaaata tatctcctgc cgcgctcccg
    14761 gacaggaacg gtttacccgc ttaaaaactc tcggtgcaga ttatacagag gaagcaatca
    14821 aagaacggat tgcaggcaag cgcagaaaga cggcgaaagt tcccaaagag cagcgcggcg
    14881 tatcgcttct tatcgacatt gaaaacagta ttaaggcagc gcagagccgg ggctatgaac
    14941 agtgggcgaa aatccataat ctgaaactgg cagcaaagac tatgaatttc ttgacggaac
    15001 atcagattga gcagtacgca gatttagtca gccggattga agaaataaat gcggaaaatg
    15061 aaaagacagc agaagcatta aagagtgtgg agaagcgtct tgcagacatg gcggtgctga
    15121 tgaagcacgt tgccacctat caaaagacaa aaccagtcta cgaagcatac cgcaaggcaa
    15181 aggataagga cacataccgg gcaaagcagg aaagcaatct aatactccat gaagcggcag
    15241 taaagaccct gaaagcggcg ggcgttacta agcgccccaa cctcgccgct atacaggaag
    15301 aatacgggaa gctccaagcg cagaaagaag ccctttacgc tgattatggc aagctgaaaa
    15361 aacaggttaa ggaatacgac gttatcaaac agaacataga cagcatttta cggcagccaa
    15421 gagagccgga acgggaaaaa ggtaaagaac gtgagtagct ttgttcatac ttatgtgata
    15481 taatagttca tatcaaagaa gtttcgtagt acgacaaatt tgaaattttc cgaatagcct
    15541 aaatgaaacc gttggttgac acttttccaa gcataggtgg tggtaatgca actataaaaa
    15601 atgctatgat tttgcaaaag ggaaaggaga attgacgatg aatatagcag acagaataca
    15661 atatttacga aagcaaaacg gatactcaca agaggaactt gctgataagg ttggcgtatc
    15721 aagacaagct gtatcaaaat gggaaagtga acaaagcaca ccagatttag aaaaagtaat
    15781 tattatgagt gaactttttg aagtcacgac tgattatatt cttaaaggga tagaaccagt
    15841 atcaacaacg aatagaaaga ctataagaac attgtatgta ggctttgctt taatatttgc
    15901 aatcatagca ggaatatggt cttttgctgc aaatagattt aattatgatg aaatcattct
    15961 aattacttta gcaggtggtg ttgttggttt tggaatagct ctaattgttc aggtaatagg
    16021 tggtatgatt tcaaataaga aacaatagtg aaaacgaaaa ttccagtttg tagaactgga
    16081 aaatttgaat tttggagatg cctatgaaca agtttttgaa tgatataaga agtgcagaaa
    16141 atcccatatc tagtaataga aaaattataa ataccatagc tgtattgttc cttggaatag
    16201 ctttggggac tttttcaaaa tttttggatt ttcgtcaagt tgaacttcca agtgtgctta
    16261 tggcaataga tggagcatta gatgttcata atttccttgg gcgttttgca atttgggtat
    16321 tgattgcact gtgtatttct atttatagta attctgcaat aagagcaagc gtcaatgttt
    16381 ttgcattttt tgtcggtatg gttacaagtt actatttgta ttcaaactat gttgcaggtt
    16441 ttttcccaag gagctacgct atgatttggt ttggctttac cattatttct ccgttcttgg
    16501 catttgtctg ttggtatgcg aaagggaaag gcaaactagc atttatgcta tcaatattga
    16561 ttttagcagt attgtttaac atgactttcg tatatggatg gggatatttc gaagcactct
    16621 ctgttttaga attggcagtc tttattattg gacttactgt tttgaggagg gacacattga
    16681 gaagttctgt gctaatggga acaattagta tagttcttgc atttttactt gatatggtca
    16741 ttccattcca ttttggataa acaacttaca gtttgtcaaa taaaaggaag tgtcagagaa
    16801 tggaacagga aatgccggac tatgaaacga tacgcgccgc cgttacgggc gaaaaatggg
    16861 cgttggaaaa agtgcttgat tgttacggcg acgaaatcaa ccaccttgca atggtaaaaa
    16921 agcgtcagcc ggacggaacg acaaaagaag aaatcgacga agatttacgg caaacactca
    16981 tactgaaact gttagaagct atcccacaat tcccaatcga aagggagtga taccatgaca
    17041 cgcggggaaa tttggaaaga ctttttcaag agaactgttt tgcccgccgt tatcgcattg
    17101 tttttattct tcatgttcaa gaacgtcttt acagaggacg ggcagaccaa ttacttttat
    17161 gtatggctgt gctgcggtat tccctttggt attcgccgta tgttcgtatg gcttgtaccg
    17221 catgggtatg acatagccgg aacggtgggt atcgtcgccc tcaactttat tttgggcggc
    17281 attatcggcg gggtcatttt gatatggcgg cttgtcgtgg ctacatggta tatccccctt
    17341 acgatatatc gtttacttac cgcagataag ggcgcaaccc ctcaaatcaa catggaaaaa
    17401 tagcaaagaa aaaaccggga cgcagacagt cagtaaagac tatccgcgcc ccggtttcct
    17461 tatgggtgca gttatgaaat gttacgcagt agaaccccga tttcaaggga aaaggtattc
    17521 tgtatcgcct ataccagttt cacgccgttt atgccctgta aatcaaggct tttttcgccc
    17581 ttattgcgta accgccgcgc gccgtttcct catgcgctcg gctgcctgtc tgcgggtaat
    17641 acgctttcgg cagtccgggc agtatttgac gctgtttgat tttgatacaa agaatgtgcc
    17701 gcactcacta caacgctttt tgtcctcggt cttgaatagc tctgcataca gcagcttatc
    17761 aagaggaagt acggcaatct gaaaccactt gcataaaagt gaataggaaa taagctgcgg
    17821 acagacacat tcctccccgt cgtccagtaa gatacagtgt ccgcgctcgc agttacagca
    17881 ctcctttttc acaaggctgt tgactttccg gctctgtggc ggcgtgagcc gcttaacccc
    17941 ggtcatacct catcagcggc gaaaatggca agctccgcat actcgttttc tgttagtggg
    18001 gagatttttt gtaaggtgcg ctctgcaaag gggcgcatat cctcgtccat gaatggcaac
    18061 gcagccgtca tattttcaat aagctgtacc ttgcttccct catggtaaat actcaacagg
    18121 ttcatttctt caacggtcag tctaatcatg ctcatacctc caattcgtga ttttttgttt
    18181 ttgccgcttt tttctgtgcg gtcttttctt tgtctgctct aagctgcgcc cggatagagg
    18241 gctttttctc cggttctgtc tgtttctttt ctgctttgag ggcttcggcg tattctgcaa
    18301 gggaaatctg tccgccggct tttgcctttt cctccagttc cgcagcggtc ggggtgttat
    18361 ttatctgccc gtcaatcatg ttgtagtttt gctcggtggt ctgctcggca gttttcagat
    18421 agttttccgg ttgctctgcg gcaatggcaa acgcccgcgt tccgttcccc ataatcagat
    18481 attttccgtc gtcggactgg tggtgcaatc catatccggc agcttccatt tgcgcgcggc
    18541 tcatggcggt tacatagaaa gtccccctcg gcgttttgat tgtttcgcct gtttccaatt
    18601 cgtcgggaat gagggctttt tcctgctcct taaaaaactc cggtacttcc ttataaccga
    18661 agctgtctac atagtgggcg gtgtcctgtc cgttctgatg aagcaccacc acgtcggaaa
    18721 cggaaaggct gtgtccctta aaatctttcg ggtggtcgat attaaagcgg gtgtaaatat
    18781 cttcaaggga agtccctggc gtaagaggtg cggaatagac aagcgcatag tttttcacgt
    18841 ctacccggta tcctgcggca gtcagacggt cgtaaggttc aaagcgcaaa tcccttgttt
    18901 cgtccccgcc ttttatctgg taaatggaaa aggtgtcttt gtcctgtggg gcttcctgtt
    18961 ccggctgccc ctgtgcgggt tgttccagtg cagctacctt ttgcgcccgg atatgcgccc
    19021 gctgtaactc ggtcggcttt tccccggtaa gaagtgcaat cgccttgata taatcagccg
    19081 cataataggc gttactggta agctgccgct gccatgcccc ggcttttggc gaccaacgga
    19141 agccgttgct ttttaaggct tcccgcgtgg tttcgtccgg cttatcctca aagaagattt
    19201 gcagtcggtt cgcttcggtg tttgcgtcca ccttgccgcc gtcaaattcc caacccacaa
    19261 aaccgatttc tttttgctgt gaaagggatt tgatacggtc tttcacgcgc cggatttctg
    19321 cgctgttgtt agataacgcc caagtcgcaa agggcttgtc ccccaaatgc caactgctcg
    19381 ccatgtctgc tttcagtttt tcaagctgtt ccggcgacaa atgcgggcag ccgtcaaggg
    19441 tcttgtgctt acgataatag gcgtttaccg ctttcatggt ttcctgtgat ttttcaaggc
    19501 tttccagttt cttttctaac ttctgtactg cctgcgggtc gtctgcgctg ataccgccca
    19561 taccggtact gcggatttta tcaagaagcc cctgtatgtc ctgccattcc cggtaattgc
    19621 tgtcgcgggc tgcgttctgc ttttctttct tccttgtggg aaagttagag ccgcctgcaa
    19681 tcaggataga gggaacacgc gcgtcaatct catagccttt gttcatgttc gccgccagtt
    19741 tccgggcgta ggtatcaagc aggctgtcga ttttatcatg gtacatcggg tctacccgct
    19801 gtttctgcct ttctgcaagc tgtacggctt catcaacata atgcctgtat tcagccgtcg
    19861 cgctgccttg cttataatcg gaaaaactgt tcatatcctt tgcacgtttg gcggcagctt
    19921 cattgatagg ataataaggg gcggcgggtg tagcctgttc cggctgtggg ggctgtggtg
    19981 cttcgctgct tgcgcctgta tcagcttccg gctctgtctg tgcttctttt tcctgtgccg
    20041 gggctatttc ttcgggaacc tccggcgcgg ctgcttctgc ttcgggctgt ttggcggctt
    20101 cctgtgcggg ctgcgcctgt tcggtggtct gttccttatc ctgtgccttt tggatttctg
    20161 caaaatgccc gtctatggta tcaatcagct tcgcggctgt gctgcggatt gtttcaagag
    20221 agcctttcag ttctgcaagt tcttttccgc tgctccaccc ggcgatatac ccaaaagaat
    20281 agtccgacgt atcaagcccg taatgttggc agacagcata ggcgacgctt tccgcctgta
    20341 cctcgcgggt gcggcggtcg gggcgttccg gcatggcaag agcggtatca tgcagcgtgg
    20401 cgtgggcgat ttcatggatt gcggtcttga tattctgcaa ttcgtccata ccctcgttga
    20461 tgatgatacg cttttcctca taattgcagc gtccttttac gccgccgctt agtgtttcaa
    20521 atcccataac aaacggggaa gttttttcaa gggcggcgaa aaaatccttg tattgttcca
    20581 cgttacccgt cagctcatag gtaagggcag gaagctcctt tccctcggtc tgtgaaatat
    20641 caaagacaga taccacacga aaagcgggta tcttgacttc cttttcctcg gtaacaggct
    20701 tcccgtcttt atcgaaaaca ggcttttggg tgtccgggtc gattttttcc acctgtttct
    20761 taacggtaaa cggggacggc gcaagtatct tgatagcttt ttctccgggc tttacatggc
    20821 ggtcaaattc cttttcccat tgcttatagc ctttcacaag attgccgcct tgcatggcga
    20881 taaggattgt attgttaaag ctgtaattgt gaaactttga catggtacgc agataatcgg
    20941 cgtagcggtc gctttcataa atccccaaaa tgccctgttc cagtcggtcg gtaatctctt
    21001 tcattttatc ggcgggcttt tcagaagata ggataatcgg gagaaccggg ggctgctctg
    21061 tggcaaatgc aggctgtacg gcagcagcgt caaggtctgc ctgttccttt tcctgtactg
    21121 gcggctgcgg cgttacccgg tattcctccg gcacgtcccg cccgtcgtac cactgtgtaa
    21181 aggtgttgcg gttgttgtag atatagccct gctcggtaaa tctgcccccg tcattgatag
    21241 cagcgtcccg cccgtattcc tcatacatga aatatttttt tgcttcttcg ggcagctcca
    21301 actcgtccag ttcttcaatc agataatgcc cgtaatcttc ctcactctgt acggacggat
    21361 aaagccagta acaatcaagg ttttgtgcaa ggttgatgat gtcctgcaaa ttccttgtat
    21421 ggtcgccgta tgccatagct gcgacaaatt tttcccggtc gtcgtcaaac tgcattttca
    21481 aaagttcgcc caaatagttc aactcgtcaa ggctttcaag ctctgtcaat ttccctgcca
    21541 gtcccgcaat agaggactgg tattctgtga tatgtagctc cccgtagttt ttgccgtcta
    21601 tcccgatacg gtcaaagatt tctttcaaat gctcggcagt cgtggggaat gatacccatt
    21661 cgcccgcagg tctgccctcg gtgtacttcc caagattaga aagatacccg cttaaaatcg
    21721 tttcttccat gcgttcactc ctttcgtttt caatcatgcg gtgcataagc tcgtagtcct
    21781 ccatgctcat gctcccgtca aatagatagg ggtcgggttc ttcgccgtcc cggtaggctt
    21841 tttcagaaaa aggcagcccc gcagcgtggg cggcgtctct tgcttcctcg ataagctccg
    21901 gcggcagcct gtcgtcgtgg gcttcgataa attccccgat attgctatat ccgttttcgt
    21961 agtggcgacg caccccggcg gttagttcgt caaggttcct atcctcgcca agatagccgg
    22021 aataatagcc gtttaccaca acggcggcag ggtcagcctg cttgatttct tccatgcggc
    22081 ttctgtcctc cgggtagagg tagtcgccca tttcaaggtg gaaatattca gcgttccaag
    22141 aacgtcccat tttccaaaac gccacccaag cgataccgtc ccgtaattct tcttgatagt
    22201 cctttacggt gtcccgtaaa cttgccatgt gtaacctcct ttccaaacgg cagcgcggac
    22261 gctgtatgtt ccggtttcat cattcagtac cccgcgcccg cgtttttgcc aaagagttta
    22321 gatatttttt caagatttca gagggtaaaa ccctctacaa aacagagggt ttgggacact
    22381 tcccaacaag caaaaatccc cgccgttctc ggtagagaaa gcagggattt tacggaaaag
    22441 ggtactcttt tcctatgctt gctattcagt tattttttct tcggaagctc cacccggtag
    22501 ttaacgtatt tcccgcccgt atcagctaat acgatagttc cgtcgtaggt tttgcccgtc
    22561 tttggggaat agagcttttt cacatttact ttgccggatt ttaagagtgc agcggcaatc
    22621 ttcggggtaa aggctacttt gcgttcctca aagaaacggt catttttcca catggtaaag
    22681 atacattctt tgttgctgca atagtaattc ttcttgccct cataaacggg agaaccgcag
    22741 cgggggcagc ttcccaatgc ttcccgttcc ggtttgaaca tctgcgattt atcatcagaa
    22801 agaaacggat aggtctttac cagttcccgc gccatatcct caataccctc cataaatacg
    22861 gcggggtctg ccttgccttt ggctatctgc gtcagattgt tttcccattc tgcggtaagc
    22921 tgcgggcttg tcagagtgtc cggcaggaca cacacaaggt tgataccgtc ctttgtggga
    22981 agtagctgct tgccttttcg ttccacaaaa ccgcccttaa ccagtttttc aatgacggca
    23041 gcgcgggtgg cgggcgttcc aagccctttg cgttctgcgt ccgggtcggt gtcctcactt
    23101 ccggcgcgct ccatagcaga gagcagcgac gcttcgttgt ggggctttgg cggtgtggtg
    23161 tcatgctccg ttaccgttgc cgccggattt tcaaaggtct gtccctcggt aaatggtggc
    23221 aaggtctttt cagtgtcgct atctgcgttg tccgcttcgg gcttgttctt taaggctgcc
    23281 ctgtattttc ggtcaatctc tttccaaccg tcggacagca ccgtttttcc ccttgcggta
    23341 aaagattgtc cggcacattc aaaaatcgcc gtaaccgctt caaaggtatg cggtgcagcg
    23401 gtcgccataa gcagacgcgc cccggcaaga gtaaggatat ttttttcgct ttccggcagc
    23461 gcggcaaggt cggtctttgc aagctccata gtcgggataa tagcatggtg gtctgatacc
    23521 tttttactgt tcagcacctt tccaagctcc ggcgtgaagt cttttcccgc cataaaagga
    23581 aatttcccac aaagcagctt gataatgccc gccgctgtgt cgcccatgtc gtcagtaaga
    23641 taactgctgt ctgttctcgg ataagtaaga agtctttttt catacagggc ttgtgcaagg
    23701 tcaagggtct gctttgccgt atatccgaac aggcggtttg cttccctctg taaagaagta
    23761 aggtcaaaga gcttcggagg ggctacggtc tttttctctt tcacaaggga agtgcagact
    23821 gcttttgacg cttcgcaagc agctttcagc ctgtcagctt cggaacggtc agacagtttt
    23881 tcacttgcag cttccgcgtc ggataaggta aggcgcacat ggtagtattt ttctttcttg
    23941 aaagtggtaa tcgctgcgtc ccggtcaaca agcattttta aggtcggggt ctgtacgcgc
    24001 cccacgttca aggtatgatt gtaaaggaca gagaaaaggc gggtggcatt gataccaatt
    24061 aaccagtcag cctttgccct gcataatgcg gaagcaaaga gcgcgtcgta ttcctcgccg
    24121 ttcttcagac gggaaaagcc ctctttgatt gcgctgtcct ccatagaaga aatccaaagg
    24181 cggcgcatag gtttcttgca gcccgccatt tcatagacaa aacggaaaat gagttctccc
    24241 tcgcgccctg cgtcgcaggc attgacgact tcggaaacat ctgcccggtt cataagctcc
    24301 tttagggttt tgaattgctt ttccttgtca gccgaaacgg tgtacttcca ttcctgcggc
    24361 aaaatcggta agctgtcata gctccacttt ttatactgtt ccccgtaggc ggcggcttcg
    24421 gaaagcccta ccaaatgtcc cacgcaccaa gaaatgatat agccgcttcc tgtgagaaat
    24481 ccgtctttct tttcctttgc cccaagtacg gcagcgattg tctgtgccac actgggcttt
    24541 tctgcgataa ctaaaatcaa ataaaaatcc tcctttcggg taaaagaaaa gagcaaccga
    24601 tttttcgatt gctcctaagt gattgtataa aattgtgcga tatgaaatat aaaaataatt
    24661 tgacaaactg aaaattactt aacaatatgt gctgtaatga ttgtatagct atatgaattg
    24721 atagtaattt tgatattttc aacctcgcaa taccaatttt taccctgttt gtatatattg
    24781 caatttttat ctaatacctt atttttgcaa tactcaacaa catctgctgt atctattttt
    24841 aggtttcttt taattctatc aactcccatt tcggttgtat gaattttatc aatattatca
    24901 agcaatatct ttttatcttc cattctttct tttcctcaac ttcaagtttg tttgtctctg
    24961 tcatttccta tggttggaac aattatatca tattccatat cgcaagtaca tctttttatc
    25021 gttcggtctg tcgtttattt tccagttttc tcatacagta ccccacacaa ggggactgcc
    25081 ccttgtaagg gcagcccgcg caacgtgggg aagaaagctc cggcggggat ttttcacgtt
    25141 tgccatagtc cggtttctgt atcatcatct tttcaaaggg attgtcggta aacagggtca
    25201 tagggcttct tcctccgttt cttcctcttt ggaaacttcc ccggtatctg cttccggctc
    25261 gtcctcgtcg tagtcctcaa agtcaaaatc ttcaaggtcg gtgtttcctt tcacgttctg
    25321 cttcggtttt ataaacttaa agtagtagaa cgctgcgccg ccaccaagca gcacaaagag
    25381 gactaaggca agcactgcgc cgggattgcc gcctttttct ttgggttctt ccggctgctc
    25441 tgtgggtgtc ggggtaggtt cttttcctgt gcagcctgtc agagtggttg cacataccgg
    25501 acaggacgta tttacctttc cggcttcgca tttctccgta cagttgcaga cttccggctt
    25561 tttcgtggct tccccggttt cggtcagtgc cataaggtcg gcttcgtcca cttgattaag
    25621 gaaatgtacg gtctgttcct cctcggctgc ccggtcaatg aggatataga agtagttgcc
    25681 gcctttggtg gtaacagtga taagctgctt attgccgccg aaatcgtcca ccagtgttgc
    25741 gttgcccttt ggggtaagag gtggggtttc cggttcttcc accactacat tgctgtcgtt
    25801 ggtcgcgtcc tctgcgggtg gctctgcggc tgtcccctgt gcataggcag ggacagaaaa
    25861 accgcccata aggataaggg cggcacaaag ggcagttatg gtctttttca gtttattctt
    25921 catctgcatt gtcctcctgt tcttcgtagt ctgccgcttc atcagaagca ggggcggcac
    25981 tcatgccggg aataccgccg ccggacagca tagcgttcag ttcctgcggg gtcatacgca
    26041 tagcgcgtac aagctgcacg atttcaaggt tttccgcttc ggttttctgc gcttccagtc
    26101 cttttaattt gttctgatac tctgtgattt tctcgcgggt ctttgcgatt tccttattga
    26161 tacggtcgat tttattgttt gccataggtc atttctcctt tctttttggg gctgttttac
    26221 cagttcatca gcccgtagcc tttgatacat tgatagttga ggtcatagct tttaatcttg
    26281 caggcgtcgc cggaattgcc ctcaacggta tagacgcggc tgccgtccct gccaagcaca
    26341 agccccacat ggtcggcaac gccgtctaaa tcccagtcga aaaagatagc gtcgcccggt
    26401 gcgatattct cataaccgcg tgcgccccat tgtccccttg actgaaacca ggggacgccc
    26461 tgccattcgc agcccgcaaa gcggggttcg ctttttccgg cttgattgta gcaccatgac
    26521 acaaagcagg cgcaccattc cacgcggctg ttaaagccgt accaagacca ataggggtag
    26581 ccgcccacat ttccgacctg ctgttttgca agttccataa gctccgggtt tccgggacgt
    26641 gtgccgttta caaactccac gccgcttaaa tcctcggacg ggcttccgtc gggagagccg
    26701 ccgccgaaaa tcaacggttt gttgccctgt gtctgtaagt acaccttgaa catttcaaac
    26761 tgctgtgggt tcaacagttc ttccgcaagg gaagcaatcg gcttgtttgt cagtttcaca
    26821 tttaagatac ggtattcgta ttcttcctcg ttgccctcct cgtcggtggt agtgcgtatc
    26881 tcgatttcct ccgtcagcgt caaggtgtac tgtaagccga acagccgctt tagctctgcc
    26941 tgtgcgctct gcggggtgta actctgtaaa agggcagtca agtacgacgc taattcgtgg
    27001 gggttatgcc cgatagtgtc aaggtcatag cggtattcat catagccgct gtgggtgctt
    27061 tcgatattgt caatttcctg ttgcagctca ttttccattg cagcatagct ctgttccgtt
    27121 gccacaaggt cgctgtcctc cgacgtgtag gaagtcccta acacgctgtt catgctgccg
    27181 gaaaacattg ccccgcagga ggaaagccct acggatacca tgatgaacag aagcagcgcg
    27241 gcgatagcga ttatcacgcc cgccgggtgt cgccccgcaa aggcagtcgc tttctttgcc
    27301 gctgtctggg acgctccggc ggcagctttg gcggtggctg cgccgccttt ctttacggtc
    27361 tttgcatact ggcatttgat tttttgtttc tgcatgaacc gggaaaaagg attgcttgta
    27421 agctgcgggt tttcctgcaa ggctttgtgg tactggaagt tgacgtttgc cttaaacgct
    27481 gcctgttctg cctttgccgc ttcccggtaa ggttttagct tatggctgcg gtagccctgt
    27541 ttgaatttcc ttgttccgta ccttgcgccg cgctcggcaa gttcttccga tttatgcgcg
    27601 ccctccacgc cggaattgtc tttctcgaca ctgtggattt tgttgtgaac aaaaataccc
    27661 gcttcctgcg cggggcggga taacgggtta ctgtgggctt tgccgccgga tatcggcttt
    27721 tcctgttcct caaagtgcag acgggtcttt gccttgcctg tggcttcctc aaaggtgcgc
    27781 tccttaacca gtttcttttg cttcgggata gctgcctttg ctgcgtccag ttggtcggcg
    27841 gctttttccg attttttgat gtagggttgc agttttggcg tttcccgttc ctcgtcggta
    27901 aactgcaagc gggaagtgga agtacgcaaa acagtcgctt cctgcgcttt cttgactgcc
    27961 tttttgcttg cctttcgggt atgggctgcg tcaaggtgtt ccatgacgcg ctcggctgcg
    28021 gcactgtccg gctgtgaaaa gtcgctgtct gcttcccggc tgctgatacg ctcggcggtc
    28081 tgctgtgttt cattgatttc taccgcaccg tcgcgggtca ttttctgtgt gattttatcg
    28141 cgtggtttta actgtttcat aggctgctca cttcctccgg cttcgtcgtc ataatgcggt
    28201 aaagctcggt atctttcggg aaatggtcta caaagggcag taccacattt ccgtaaaaga
    28261 taagtccctc gcccgcttcg gtgtgggtta catacttcat ctgctgtggg ctgatgttta
    28321 actgctttgc aaggatagcc cggtcgccct gtgcctgatt gagcatgagg acaaaatcag
    28381 agttttcaaa gatattttcc acctcgcggc ttgctaaaag gtctttgata ttctgcgtga
    28441 tagcggtcgg tatgccgccc catttacgaa aacgtttcca aatctccaca ctgtaagcgg
    28501 cggtctgttc ctctttcaag agcagatgaa attcgtccat atagtagcgg gtggactttt
    28561 tctctgcccg gttgatagta acgcggttcc atatctggtc ttgcacaatg agcataccta
    28621 actttttgag ctgctttcca agctgcttaa tgtcaaagca gacaaggcgg ttagaaagct
    28681 ccacattcgt tctgtggtta aatacgttaa ggctgccgga aacatacagc tccaatgccg
    28741 ccgcaatgcg ggcagcttcc ggctccggct gcttcaaaag ttcattgtaa aggtcgccca
    28801 agataggcat tttctccggt acagggtcag caaggtagtc ctggtacaca ttccttacag
    28861 cgcggtcaat gacggtctta tcaacgggct gcaagccctc cttgccgcca atgacaagct
    28921 cgcagaggga aaggataaaa tcacttttca gtgcaagagg gctgtcgtcc tcgctgtaat
    28981 tgaggttcat atccataggg ttcacgtact ggggtgtacc gtccatgcct ttccctgtcg
    29041 gggataagcg tatcacttgc ccgccaagcc gctttacaag ggcaaaatac tcggcttccg
    29101 ggtcgcagat aaaaatatcg tcgtcggtaa tgagaaaggc atttgtgatt tcccgctttg
    29161 ccgcaaagga ttttccgctg cccggtgttc ccaagattaa accgttggga tttttaagct
    29221 gcttgcggtc gcagagtatc atgttattgc ttaaagcgtt cagcccgtaa tacagggcag
    29281 ccccggtctg aaatagctcc tgtgtaatga acgggataaa aatagcggtg ctgctcgtcg
    29341 tcaaacctcg ctgaataggg ataaggttct cccccaaagg gatagaggac atcagccccg
    29401 cttcctgctg atagtcaaga cgggttaagg cgcagttgta tttctgtgca atgcccgccg
    29461 ccgcgaaaat atcattttcc agtttccgct tcgtgtctgc catgtttacc acaaggaacg
    29521 taaggagaaa cattcgctcg ttgcggctct gtaaatcctg caataaattc tttgcttcat
    29581 tcccaaaggt ggcaaggtcg gacgggatta tatccatgtc ataaccactt ctaaccgctt
    29641 ttttctgttc ctcaatcttc atcttgtcaa ggtctgtgat tttgcgctta atggtcttga
    29701 ttgcttccgc ctggtcgata ctgcggatat gtaagttgac gattacgcct gtttccaaat
    29761 ccaacatatc cgctaagata cggtcgttta gctccggggc aaggatttct aggaacgata
    29821 ccgcccccgt ctttttcccc atagcaaagt agcgtccctc cccgaacttg aaagaggacg
    29881 gggcgataaa gtcctttgtg gtaagccccg tcggggtcag ccagtcataa gagaatgaga
    29941 acggctcgcc tgccgggtgg aataccccat gcagcacctt taagcgttca tagccggata
    30001 aggggtgcgc cgatacgcca agcaccttga agttattgag tatgtccgtt tcaatgcggg
    30061 acagccgcgc ccttgctgcc gccggattgt cggcttcaat ggaaaacgtg atgtacttgt
    30121 gctttacaag cccgttgttc cctttggcaa gttgcgtttt cagcatatcg gaatactcgg
    30181 tgcggataga gttaaaatcg tcgttttgcg gcgggatatg gattgcccgc gctgcctgtt
    30241 cctgctgtga gccttgattg atgaaagaaa gctgcacgtt cacgcttgcg tcaaagtaat
    30301 tgaggaaatc gcaccagttt tcaaaaatgg cggtcttatc gtctgcctgt gcaagctgat
    30361 agttgatgtc ctcaaaggca atgctctttg aatacctgcg ctgtgccatt ttgcagatac
    30421 cgtccgggta catggcaagg tagggaatgg tctgctgtgc ggtgtgggct ttgccgtccc
    30481 cctttgcctg ccggataacc gccgctatct gtttcttttc cgcgcgggtc agcttacgcc
    30541 tgtttcctgc ggtttcccgc gtcttttttg tctgtccttt tgacaatcgc tgatacctcc
    30601 ttttctaatg cccgctgttt ctccaagagt gcatacaggt tttccgtctg atacggtcgc
    30661 actttggggc gggtaaattt tgtctgaatg atgttcttta ctaccacttc aaggggctgc
    30721 ccatgctttt catacatggc aaccaagaag cagggaagca taacgacaat cattgccatt
    30781 gccgcaaggc tcgtccctgc gctgtcttta agcaaaaaga aaagtggcag tcccataaga
    30841 agtgccattg aaaaacaaag gatttgccgc tttgtcagat tgaaagcgac ttttgttttg
    30901 actttggata agtctttggg tacaggtaca tacgccaaga aaagacctcc tttctgcggg
    30961 gcttacccgc ttgtgctgtg ttaatgtgca ttgaaaatgg attttgccag tgcgccggat
    31021 ttgaacaggg agaaacagag gataacggta taggctgcaa gggaaaaaat cgcgctgtgc
    31081 agattgtccg ctaagaccat ttcgcctacc aaaaccgcat aaatgccgac gcatatcata
    31141 atgagaaagc cttgaaagcc cagtgcaaac agccccttta ggtagttgtt cccaatctgt
    31201 ccccattccc ggtttgtcat ggtggcaaaa gggatagggg aaacggaaca gtaggcgtat
    31261 atctcaatca ttctgccgta gaggataacg gttatcagta cagacatgat tttcatacaa
    31321 aggcttacaa ggctcgtttc catgacaagt aacagtagtt cggggatttc catatctttt
    31381 agtccctcct gcatggcggc aagggcttcg gttacgtcga tattcgtatc gccgccgatt
    31441 acccccgccg cgccggacac aatatgctgc gccatatcaa acaccgccat agtaatatca
    31501 aaggtatgcg ttaccaagaa aacggcaaca aaggctttga aaaaccattt aaagaacatg
    31561 aatgtttcta tgtcgtgcat attgtttttt tccgttacca tgctgataag ctcataacag
    31621 aggacatagg taatgacaag ccccgcaatg ggtacaatca cattttcgga cagattttgt
    31681 atcatgttaa aaatgtttgc attccacccc tgcggggtct tgcctacctc cgctgcaatg
    31741 gtgcctactt tttcgtttac gtccccgaac atagtggaaa ggttgccgtt aatggctccg
    31801 ataaggattt cctttatcca ttcgttaatc gcttccagta tgctctccat aggctaccct
    31861 ccgtaaactt atccgaacag accggaaaga agcggtacaa ggatagtgcc gataagggca
    31921 acaccgccgc ccgccataag ctgcttaatg ccctgtgatt ttgcacccgg attgtcgttt
    31981 ccatatccct ctaagagatt gatgacgccc caaatgccta agcctgcgcc cagtgcgata
    32041 acaagggtct gtaatacgtc gatagcagag ttaaaaaatt ccataaagct cctttctgcc
    32101 gtaaatgcgg cttgccccgt aaaagggcat aaaaaacggc ggtcagtttt caaactgccg
    32161 cggtcataag cccgccggat ttcggcggtt tccccgcgct gcctatgtgt ctgccgcgcg
    32221 gcggtattca gttgttgggg tgagcgatag gaatttttgt aggggcgcgt atcctgtaag
    32281 ttgtattttt ctttgttact gcgtcaattc ctcctttcgt tgctgctcat acagggcttc
    32341 acaccgtttt acaaactgct ggatagctgc ctgccactgt ctttcctgcc actcggcaat
    32401 ggaaataagc ccgtttatgg cttctgtgat tttcttgtcc tttttctttc ggatacgtct
    32461 attcatgggc ggtgtcctcc tgcaaatctg cttcgctgat ttcgtaatag tcaaagggtt
    32521 cgtccgactt tacaagggca ggtctgcgcc gtaggtgctt ttccatatca aaggtatttt
    32581 tcttgtcata gtcggacagg tatttgtagt tggggtgctt tgtaatgtcg tacttatcag
    32641 agaagaacgg gcgcacccct cgtagctgca aaatacattt cccgccgtcc atgactgcaa
    32701 tttcatcttc ggtcataagc tgcttgccta acttctgata attcagccca tgcgatacct
    32761 cgcgcccccg gttctcggaa gtattgaagc tgtcaatggt ttctttcccc aagatttccg
    32821 acatttcttt gagggtggtt ttctccttgc cgcccaagaa cagggtggtg tcgcagttgc
    32881 cgactatggt atcagcgttg tctttgtata tcgcttttag ctgcgactgg ctttgcaaaa
    32941 tgattgaagc ggagatttcc cggcttcgga tagtggctat gagcttttca aacttcggta
    33001 tctgcccgat atttgcaaac tcgtctaaca gacagcgcac atggacgggc agcctgccgc
    33061 cgtattcatc atctgccttg tcgcaaagca gattgaaaag ctgtgtgtaa agaatactca
    33121 caacaaagtt aaaggtatcg tcggtgtcgc taatgataac aaacagggcg gtcttacggt
    33181 cgcctatggt gtcaagctcc atttcatcgg tttccataag gtcgcgcagt tccttaatat
    33241 caaaaggagc aagccgcgca ccgcaggaaa tgaggataga gcttcgtgtt tttcccgcag
    33301 ataacaggaa tttcttatac tgccggacgg caaaatgttc cgggtctttt tcctccaacc
    33361 gttcaaacat gaggtcaacg ggggactgaa attccgggtc gtcctcgcgg gcttccgacg
    33421 catttatcat ttcaagcagc gtcgtgaagt tcttttcttc ctccggggct tcataccaaa
    33481 tgtagccgat aagggcgcag taaaagagcc gttccgattt tacccaaaaa tcctccccgg
    33541 ctttttcccc gtcccctttg gtgttggcaa tcaaggtgtt taccagtttc aaaatatctt
    33601 tctcgctgcg gagataggca aagggattgt atttcatact ttttttgaag ttaatcgtgt
    33661 tcaatacttt tatccggtag ccgccccgct gtaagagctt cccacactca actaaaaccg
    33721 ttcctttcgg gtcggttaca acgtagctgc tgtgcatttg cattaagttg ggcttcacaa
    33781 aaaatctcgt cttgccgctg ccgcttcccc cgataacaag gatattttta tttcttgcat
    33841 acttcggctg ctttgggcgg ctgttcatgg taagccgttc cgtctgtgtc agcagcacgt
    33901 tattttgaaa taccgggtcg gtgtagggct taatatcttc ggcagttccc caacgggcag
    33961 agccgtattc gatacctttc cggtatttct tcgcgttctt gccttttaca tagacaatca
    34021 gccggataag caccgcgcct aaaattccta tcagtaaatc cataggggca aggctcggaa
    34081 gcgggtttga aaaagccata gaaaagccct ccccaagcga cagcaccttg ccggacaggt
    34141 cagctcccgg cgcaaggcgt accgcctgcc cgactttatc aaagagatac acaaagagca
    34201 gatagggaat gttgggaagc agcagctttt tccagttgat agcgttcatc ttgcaatccc
    34261 cctgtctttg ttttttactt tgtcccgctg tgcgttcaaa gccgccgcct tttctttgag
    34321 ggaaacaatc agctttcgga tagagggctt ttgttcctgc gtcagcttct ttgcggaaaa
    34381 ctccttgaaa gctgcggtca gtgcgtccgc gtcccgtccc ttgaagaaaa caagatagcg
    34441 gggcgggatt tcggtgctgt ctttctttag ggcaaagtca atcccgtact ttttcgccgt
    34501 actctcaaag gctttgatat tgtcctttgt gatttcaatg ttggaaacgc ccgcgttctg
    34561 cttcataagc tgctttaggg tctgtttgcc atgcgggatt ttctgtctgt catgctgctt
    34621 tttcatctgt gcaagcaggg ctttcatagc cttttggagc tgctgtgccg tcagtttccc
    34681 ggttttgatg taaagggcta tggtcttttc gttggtttcc tcctgcaatg ggtgtcctcc
    34741 tttcatgttc tatggtggta tgcccgccgc cgggaagcgg ggcgggcatg aaaaaagagc
    34801 gtccgttttc acgccgccct gttaaatccg tacccgcagc ccggaaaggt ctgccgcccg
    34861 aatacccaca agataccagt tgtccccgta ctccatacgg gtaggcttcc atttgccccg
    34921 cacaaagacc tccatgcagt cgccgcaatg taagcccccg tagtagtcgt ttacgtcaaa
    34981 gcgaatgtcg tagcggtcag cggtttcatc aaaaatcaaa gctcctgttt tctgtgtcat
    35041 aatcaaatcc tcctttgggt tggtttacag gctttcgccc tcgttgcagg tgtaatcggg
    35101 tatgccggat tttttcggtt tcccataaaa agtaccatct gccatatcgt gagatacaag
    35161 ggacgtgtaa tagctgccaa tcgtagacgg ggcattgaaa agggcagctt tcatgtactg
    35221 cttgatgttg cggattttgg tggtgttttc ctgcatacag tcaagtacaa agcggatatg
    35281 ttcgctgttc agtttcataa accttgcttt tacaagctct gccgggtagt cgtccccggc
    35341 gatacggatt gtttttcgtt tggtgcagac ggtttcaaga ataagggcaa ggatttcctc
    35401 tatcctgtcc cggtcaaggt atctgtcctg tatcagatag tcgtactcaa tattgtcctt
    35461 gatgatttcc tcatacacgc tgtatgcgtc tgtcgcttcc tttcgcttcc gttccggcgg
    35521 ctgtgccgcg tcctctaagg gagagggatt tagggaatgg aaaggaatgg aatgggtact
    35581 tgatacatct gtatttaatt tttctttttt gggtaagtta gtttttaata gttctttatt
    35641 taattgcgtt ggattttccg acgtcgggtt atccggtgtc ggattttcca atatcggctt
    35701 atccgctgtt ggattttcca ataccggaca atccaatgct gggggctgtg gctgttcgta
    35761 aatggtgtac tcgatagcgg tcattttgcc tttctcgtcg cgtccctgcc gccttgtgat
    35821 atatccggct ttttcaagct cccatacagc ggtgcggata gcgtcgatac tttcccggtt
    35881 gataagggac agccctgcaa gggtgtagtc ccaatcttcg ggaagtgaga gcatttgcga
    35941 caacaagccc tttgccttta gggaaagttc cttgttgcgt aaatggtggt tgctcataac
    36001 cgtataacct ttgttctttt ccacgcggaa aactgccata gcttcatcac tcctttggtt
    36061 ggatttgtta cgcagtagaa acgcggtttt gagggaaaag gtatcaaccc ttgcatttct
    36121 catttccgcg ctctaagagc cgcataaatc aaggcttcat ttgcccttac tgcgtaacat
    36181 gagggcataa aaaagcagcg ttcctgttct ccagtgtagg agtgagaaac gccgccttat
    36241 aggtggtgct tattttattt tttcttttcg acgtgccgcg taaggtggtg tagcggtcta
    36301 tccggcagcc cgcaggtgct ttttgcatat caaggctcaa tccgttaaaa gtagtaaatg
    36361 agtagtaaaa tcaatttgca aacctgcgaa tatgcccgta aatcaagcat ttttcaagac
    36421 aatcaactaa ttttaacaaa acttataaca tatatttttt ataatatagt gcatgaaaaa
    36481 tttatttaac taattttaga tatttattga caactgattc agccaagcat tttgatgaat
    36541 caacaatatt tattgaagga tatgattttt ctaaaactac atttaaatca gtacaagcaa
    36601 ttactatatt ttctatagat tcatttttaa cgtcttcgat aagtttattc caaatatcaa
    36661 tattgtgtgg attttcttta tccattttta tattttgaat aagattgttt aattttattt
    36721 gccattcatc tttaaatata aattcatgtc cattatgtat tataccattc tggtaaattg
    36781 tagactcaaa agttgaacta gtcgaaaaaa gagttacttt ttgtgagata gtaggtaatt
    36841 ttttaactgt ttcttctaca atatttaata ttggtatatt aatagactct tttaattctt
    36901 taaagtatat atgtgctgaa ttgcatggca ttgcaataaa atttacacca gttgattcaa
    36961 gcttttgtaa tccatcaatt atagtttctt tcataagttc atgatttatt ggacggtcaa
    37021 tataaaatgg ggtaggtaat gaatatatca tcatctttgg aaattcatca tcatattttg
    37081 ctccatattg agattgacac tcatcaataa ttaaatcaac aaaaggggct gttgaccttg
    37141 gacccattcc agccaaaata ccaattaatt tttcattcat aaaaattatc tcctttatat
    37201 ataaatatat ttatgtataa aataaaaatc tataaaagca taaaattata ttttataata
    37261 gattatattg tacacttata taatataaat ataaagtgat tttttagatt atatatttta
    37321 atatactttc ttatataaga attattttaa atcaagatta tctagtattt ttaaaaatat
    37381 ttttacagca gtatccaagt catctacaga aacactttca ttaggattat gacttacgcc
    37441 cccagcgcat cttataaata ccataccaat atcagttata ttatccattt cctgagcatc
    37501 atggccagca ccactataaa tataaaaagg attaagattt aaatctataa aacttttttc
    37561 tatgattttt gttattttat tagaacatgg tacaggaaca ttttcaaaag caagttcact
    37621 tgtataatta agttttctat tttcacatat atgtgatatt tcattaaata tcttttctac
    37681 agaattatca agaatttctt gtgaacaaga gcgtacatca agagtaaatt cagctctttc
    37741 aggtaccaca tttgatgaag atggatagaa attcatcttt cctacagtag caaccaaatc
    37801 agcagtcgtt ttagctactt tttcgatagc taatacacat tcacaggcac aacatccagc
    37861 atcaagtctc atattcattg gaagtgtacc agcatgtcca gacttaccat ttactgatat
    37921 cttaaaactt ttaaatcctg taatacttga tacaattcca gcaggaagag attcattttc
    37981 taaaacagga ccctgctcaa tatgaaattc taaatatgca tctacatctt ttctaggatg
    38041 caaatttgtt aagttttttg tattaaatcc aaactttgtt acagcttctt caagtgtaat
    38101 tccattttca tcaacagatt taagaagttc ctctttaaaa gtaccagcaa aaactttact
    38161 tcccattaaa gaagtttcaa atctagttcc ttcctcttca gcgaaagcaa ttagcttaat
    38221 attgaaagga taagacctat tattgtgttt tgcttcttca agaccaacta gaggcactat
    38281 tatacctaac ataccatcaa atatacctcc acactttaca ctatcttgat gagatgcaac
    38341 tactaatgtg ggagcatttg gaaagcttga tttatatgta cctataacgt ttccaatgtc
    38401 atcaatttca atatcaagat ttaaaccttc catccatgaa ctaattaaat caatagattt
    38461 tttatgttct tgagtcaaga taagtcttgt aagaccattt gttgttgaac ttatttttcc
    38521 taatgtttgt agataatcca tacatttttt tcctaacata tttacctcct aaaaatataa
    38581 tttttattta agctaagatt agcttttata cttataattt ttgaactttc aaataatttt
    38641 gtatgtatag taaaatacta ttttagtatt aataatgata taaaaacata aagtctgtag
    38701 acgaagagca gaagttttaa tttaaaaata tgaaatatga tttttattat tatagtattt
    38761 ttctataata atatctcttt ccagttagag gataattttc aagtgaaaat actttatggt
    38821 atccaaattt ctcataaaat ttaggtgctt gaaaactgaa tgtatctaaa aaaacatatt
    38881 tacaacctcg ttctttagca attttttcag ccttatataa aagttgactt ccagtacctt
    38941 tatatctaag cttctcacta acccataagt attttataga aagccaatta ccatgagtaa
    39001 cacctataaa acctgcaaca attttacctg ctttatctcg aatatatatt cctaaatcca
    39061 ctgggttttt atcttcaagt ctagcaagat tatattttaa taatccttga aaaatagtat
    39121 cttcatcttg tttttctatt ttttctgtaa tttctaattc catatttgaa ctttctcctt
    39181 gtataatctt ttgtatattg gtatttttta ataaaatcag tagaaagaat atagtttaac
    39241 attattctaa taggcattta tgctaatgtc aaactaataa tacttattct taattttata
    39301 ttgttgatat taaatttttt taaataaaaa tgtagtttaa gttatataaa aagaaatcat
    39361 caataaattt agtaaaccta aattgttgaa tattaaaata agttaattta atacaaaaaa
    39421 actattttag atgttattct attgctatgt tcaataatat cattagtagt tcaatttata
    39481 aaatgaataa attatataaa taagattaga aagtagtaaa tagtcttatt aaa
//
LOCUS       DAFKMX010000013        38457 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFKMX010000013 DAFKMX010000000
VERSION     DAFKMX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.013"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..468
                     /locus_tag="LZB08_000554"
     CDS             <1..468
                     /locus_tag="LZB08_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRSP domain-containing protein"
                     /protein_id="HBM7314073.1"
                     /translation="NKKYVKYTGRFGINRREQFRLKEIVKRESKKITMASKESLKNEK
                     EKILFLGTEEFMYIPMLFAKEKENQYDIYYHSTTRSPIINIDKNNYPIKSKFMLKSFY
                     NIDIQNYIYNIDKHNFSKCYLFVELNKSENSYLEFIDIIKKTSIKELTIVCCS"
     gene            522..1610
                     /locus_tag="LZB08_000555"
     CDS             522..1610
                     /locus_tag="LZB08_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBM7314074.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1638..2429
                     /locus_tag="LZB08_000556"
     CDS             1638..2429
                     /locus_tag="LZB08_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBM7314075.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2544..3098)
                     /locus_tag="LZB08_000557"
     CDS             complement(2544..3098)
                     /locus_tag="LZB08_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBM7314076.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            3294..4496
                     /locus_tag="LZB08_000558"
     CDS             3294..4496
                     /locus_tag="LZB08_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBM7314077.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4572..5552
                     /locus_tag="LZB08_000559"
     CDS             4572..5552
                     /locus_tag="LZB08_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBM7314078.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5973..9017
                     /locus_tag="LZB08_000560"
     CDS             5973..9017
                     /locus_tag="LZB08_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBM7314079.1"
                     /translation="MKKTLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSIDQISDVS
                     LSIEYVKQYTDRHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNAGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFADKDCTKQLATLTTDKNGNTDVAEVTAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYTKDNLPAETTRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKTIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSAEWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSESNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKTLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAEKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYMFTADSETMKVEVAFTFDATSL
                     NGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPS
                     RPSDSPKTGDNTNLYGLLALLVTSGAGLAGIFFYKRRKMKKS"
     gene            9256..9582
                     /locus_tag="LZB08_000561"
     CDS             9256..9582
                     /locus_tag="LZB08_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7314080.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYSIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            9598..9981
                     /locus_tag="LZB08_000562"
     CDS             9598..9981
                     /locus_tag="LZB08_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBM7314081.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSSQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNHTPQGQQKK"
     gene            10074..10493
                     /locus_tag="LZB08_000563"
     CDS             10074..10493
                     /locus_tag="LZB08_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HBM7314082.1"
                     /translation="MMFEDLDKFIAENSEEILVTAGRTDDKISELETQLDIAFREEVK
                     IFIRKYGVLIGYGVEIVACGLNGHSRFVEDTLRFRKNGLEKKYIVIEGDSELAYCLNN
                     ETGEIVNWGLDNFEVYLVAESLESFVLAQFEEGKTNW"
     gene            10568..10810
                     /locus_tag="LZB08_000564"
     CDS             10568..10810
                     /locus_tag="LZB08_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314083.1"
                     /translation="MNIKEAIKMIQDEKLQGYNMNERKDDRENEVVLMRENDKWIVYA
                     TDERASKVTNSQDIYLDEEEALDDFIDRLRAFNRLR"
     gene            10919..11140
                     /locus_tag="LZB08_000565"
     CDS             10919..11140
                     /locus_tag="LZB08_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314084.1"
                     /translation="MEECEVKIYYKGFLCNLAPYRVMGEDRHALFPVTQSNDPIFYEE
                     FDEVHYGLWAKVLTDEEYQEIVDTVTKNE"
     gene            11177..11827
                     /locus_tag="LZB08_000566"
     CDS             11177..11827
                     /locus_tag="LZB08_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314085.1"
                     /translation="MIKRIWTFKRITLAIITFMCTIIFAISLQKSIMGTDKLQRDFGV
                     AFLFIVVLVCFLYFLYRLLIPKSFRCMTVVKKYLSFRELKDRINNESFSKVVIDEKKS
                     GKIEIYYSSKWIYADEVYIPRKLVLDLIEERKSLYSSFEKLSIATKNGENIIFAIVDI
                     EEAEKIINSLQEIFEEFTLDFNNMRKIQNRLLRKEIKQEFYKRVINKEDFLKESGL"
     gene            12038..13405
                     /locus_tag="LZB08_000567"
     CDS             12038..13405
                     /locus_tag="LZB08_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314086.1"
                     /translation="MHKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFRLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQEIGRLEEKRT
                     ASNES"
     gene            complement(13411..13611)
                     /locus_tag="LZB08_000568"
     CDS             complement(13411..13611)
                     /locus_tag="LZB08_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314087.1"
                     /translation="MQVYIDLENLLKEKNISKNKVCEACKLQRTQLNNYCKNKVSRID
                     LTILAKLCDFLECSPNDILKLK"
     gene            13723..14133
                     /locus_tag="LZB08_000569"
     CDS             13723..14133
                     /locus_tag="LZB08_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314088.1"
                     /translation="MCKKRKIITITVLFVLGVAVSYLQWGREIDVAGSMGTSSENFHE
                     EHVTFTLNRLIITDKEKCAEEIIKKCRENDFTSILFSYDVAKPNALYGTVYRSRFSMK
                     GQKPLFHFRYTQTADTLGSYNIVDDPEAFTLVIE"
     gene            14372..15568
                     /locus_tag="LZB08_000570"
     CDS             14372..15568
                     /locus_tag="LZB08_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBM7314089.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFSTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKQEYIGKSRSYKFYQSGELIKHREHDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            15581..15715
                     /locus_tag="LZB08_000571"
     CDS             15581..15715
                     /locus_tag="LZB08_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBM7314090.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLEESEDN
                     "
     gene            15716..15937
                     /locus_tag="LZB08_000572"
     CDS             15716..15937
                     /locus_tag="LZB08_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314091.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            16141..17346
                     /locus_tag="LZB08_000573"
     CDS             16141..17346
                     /locus_tag="LZB08_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314092.1"
                     /translation="MMYGIIAVALIFVFANLPQYIRKQLTEKIADESSKAKENLQTSQ
                     LNFLTKIEWLKFYPLKKILLGVIWLVCLFYAVRGVFMTINDPRGVFIFLTVLFWLITI
                     FTARKIWRYIKLSYHCVPVLNHIKTKEELKESLQGECFEKVLFEHNILRKYFPVLISE
                     NWVVMNGRLFPRHGVEKIYYLHESPVWNYEQIRFIYSNGEEYLTPSDRQTVDELRQTE
                     ISNLLHKICPVVIEKAEDINTTSNKKDKSIIYWKMNYKGKFRRTLWMIPFVIILCILL
                     PLCLGSSWIIYDIIMIAILVWQLWYTYKKMKLEERTLNEIDENNNKKYTIAMKNIHMY
                     NYLLAIKDETHIYKAIIEAVPDENNDMMIWLDDFNFPQTEIEDIKKEIELYFKAKSIN
                     CIFKAGKRV"
     gene            17503..17799
                     /locus_tag="LZB08_000574"
     CDS             17503..17799
                     /locus_tag="LZB08_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314093.1"
                     /translation="MSDIFKDMQSKVGCEYISDLPSYKRKVWQEMKRLNPADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGSTCR"
     gene            17718..18221
                     /locus_tag="LZB08_000575"
     CDS             17718..18221
                     /locus_tag="LZB08_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7314094.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGIGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            18239..18742
                     /locus_tag="LZB08_000576"
     CDS             18239..18742
                     /locus_tag="LZB08_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBM7314095.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            18852..19250
                     /locus_tag="LZB08_000577"
     CDS             18852..19250
                     /locus_tag="LZB08_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314096.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNDVPDKIY"
     gene            19228..21678
                     /locus_tag="LZB08_000578"
     CDS             19228..21678
                     /locus_tag="LZB08_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314097.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGVAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            21678..23888
                     /locus_tag="LZB08_000579"
     CDS             21678..23888
                     /locus_tag="LZB08_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7314098.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKAEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQTQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTIPKPSMEKTQEVKRPATATTSKTSEPVKTSVIKERPLSSGASDRKATQSVQP
                     VHRQNVEKVVSQETRQNYKAERTTKTQTFEQSKRTTEHTKKNRNLVTKKGQKKK"
     gene            23885..24892
                     /locus_tag="LZB08_000580"
     CDS             23885..24892
                     /locus_tag="LZB08_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBM7314099.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTKSSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            24908..25819
                     /locus_tag="LZB08_000581"
     CDS             24908..25819
                     /locus_tag="LZB08_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7314100.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIKTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADNSVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVAGNVLEPIGRDCLYSELVNPIFTKDGDNVKVKFAVKS
                     LDNQTKATQVSQYELVLHKDGNWKIIG"
     gene            25908..26108
                     /locus_tag="LZB08_000582"
     CDS             25908..26108
                     /locus_tag="LZB08_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314101.1"
                     /translation="MIKNRIKEFRARYDMKQEDLAKAVGVRRETIGNLEKGRYNPSLV
                     LAWNIAKTFHVTIEEVFTVIDD"
     gene            26125..26349
                     /locus_tag="LZB08_000583"
     CDS             26125..26349
                     /locus_tag="LZB08_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314102.1"
                     /translation="MLGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKV"
     gene            26411..27109
                     /locus_tag="LZB08_000584"
     CDS             26411..27109
                     /locus_tag="LZB08_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314103.1"
                     /translation="MGSVLIIDDDKELCTLMKKCIEQEEMSALVANGGTEGLRLIDEN
                     KNLFSLVILDVMMPDIDGFQVLEKIRETSNIPVLMLTAKSGEDDKVFGLRLGADDYLT
                     KPFSIKELMARVNSLIRRYTTLNPTFAEEDCITLKGMKIDKVNRTVLVNNISVELTSK
                     EFDLLTFLASNKGRIFTKKQIYTQVWKDDYAFDDNNIMSFISKLRKKIEADPEHPFYI
                     LTIRGVGYRFNKEA"
     gene            27112..28029
                     /locus_tag="LZB08_000585"
     CDS             27112..28029
                     /locus_tag="LZB08_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314104.1"
                     /translation="MNTYLLIAFVLALVIIACLVYKLYHINEQISFIKEVLVDIKSGN
                     LNRRILVQENDITKQICYDINQIAINNQSQLIHQKQSEQAYKRLMTSLSHDVKTPLAS
                     LVGYLEAVESQIVIGKEKDEYIHVASEKAQHLKHFVEHLFEWVKLDSGEQIFHFEILD
                     LNELTRNIMTDWILILENSYFEYEVEIPEEEYQVRIDINAYTRIINNLLQNIIVHSEG
                     NKMMLRVLKDQQRIKVIITDNGKGIASDKLPHIFERLYQCDNSRSTKGNGLGLAITKE
                     LVNAHKGTITANSILNHGTEFILSFPKAL"
     gene            28181..29104
                     /locus_tag="LZB08_000586"
     CDS             28181..29104
                     /locus_tag="LZB08_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314105.1"
                     /translation="MSDTVIKTKQLTKKYGKQTVVNKVNLNVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTITTSGNVEVFGKNIDGNEKRIYSRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTASPNAVKHALEVVGLPYKDKKTFAKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEVRDFIKKLSVEHGKTILISSHILSEIAVLADDIGIIDKGVLLEESSM
                     SELEKRNRKYISLQVSDIPKTILILERQFHVKDYSVQDEQTVQIYDTTLNMGEVNKAL
                     VMEDITVISSGLCNDTLEDYFKKITGGEGIA"
     gene            29097..29831
                     /locus_tag="LZB08_000587"
     CDS             29097..29831
                     /locus_tag="LZB08_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314106.1"
                     /translation="MLKLVKLEFCKLRRKKLIWLMLLSAFVMPFFAFLYFGYLGQTNI
                     DPRLFYKWSAFGCTLFIILPFILGLFSTMLMHDENRYDMLKQLWIVPVSKMGYFFSKF
                     FVVLIYSVCFMLITAFASVLFSVIPNYVVFEWSSVLFLLERCLEIAVLTSFAILPILA
                     ISSSQKGYIFPLCITLIYVFAGFLITPINSCLHPLSCISVIIARNEAILGLSLPQNNV
                     PLALLSICVWGIASTLLAKIKLSTRK"
     gene            29841..30590
                     /locus_tag="LZB08_000588"
     CDS             29841..30590
                     /locus_tag="LZB08_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314107.1"
                     /translation="MRILLWAEFQKLRRSNIIMFTIFSTILIAVIVLISGVTTVSEEQ
                     LTTAMAGWYMTITQVWATMFVLPAVIALLGSYMICREEQDDTLKSLRLIPVNETKLTT
                     AKMIVALVFSMLVYLLLFMITFVTEAILHFSDLSMAMFSEFLKMYLLEGIGVFFAVSP
                     IIAIVPYLKKSYWLALLLAEIYSFAGLFMSMSNTLKTFYPITAIFGISGYYETTIQDY
                     FCSLGILIFCGCISAILLKNLNHRQRKKANE"
     gene            30583..30774
                     /locus_tag="LZB08_000589"
     CDS             30583..30774
                     /locus_tag="LZB08_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBM7314108.1"
                     /translation="MNKLCRIRKGKIFFGVCGGLAKCFNVDVKIVRLLFTITGFIFPK
                     SIIAYIILMIVIPKEPKTR"
     gene            complement(30895..31254)
                     /locus_tag="LZB08_000590"
     CDS             complement(30895..31254)
                     /locus_tag="LZB08_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314109.1"
                     /translation="MRKNKETQTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPSEKVEKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            31486..31665
                     /locus_tag="LZB08_000591"
     CDS             31486..31665
                     /locus_tag="LZB08_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314110.1"
                     /translation="MITYRRTLRRSQLDNYFCLVLQSSYPLHELKIHIDSIYITVCLC
                     SYIQMGCFYFIEIIF"
     gene            31765..32184
                     /locus_tag="LZB08_000592"
     CDS             31765..32184
                     /locus_tag="LZB08_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7314111.1"
                     /translation="MKTSSFKQAIEAQFDCLSKKVIKRAVKKGYRDMKRREKRECPFS
                     DIPDYEQERFGTFDKYESDYTVFNILGIEVWVEDDQLSEALKTLTEKKRNIILLSYFM
                     DMSDSEISEFIKIPRSNVQYHRTKTLETMKKIMEERK"
     gene            32181..32414
                     /locus_tag="LZB08_000593"
     CDS             32181..32414
                     /locus_tag="LZB08_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314112.1"
                     /translation="MKKHEHLPFEVILSATEGEPEAVETVLKFYDGYISKLCLRKLYD
                     EYGNVCMVVDADLKNRVQTALLDMLMNFEIVVM"
     gene            32806..33009
                     /locus_tag="LZB08_000594"
     CDS             32806..33009
                     /locus_tag="LZB08_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBM7314113.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            33088..34278
                     /locus_tag="LZB08_000595"
     CDS             33088..34278
                     /locus_tag="LZB08_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7314114.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRLYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRQLPLTE
                     RAYQAIQRILKNREKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            34570..35523
                     /locus_tag="LZB08_000596"
     CDS             34570..35523
                     /locus_tag="LZB08_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBM7314115.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            35513..36469
                     /locus_tag="LZB08_000597"
     CDS             35513..36469
                     /locus_tag="LZB08_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBM7314116.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMMFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            36466..37221
                     /locus_tag="LZB08_000598"
     CDS             36466..37221
                     /locus_tag="LZB08_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7314117.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSIQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            37313..38278
                     /locus_tag="LZB08_000599"
     CDS             37313..38278
                     /locus_tag="LZB08_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7314118.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        13379 a         5896 c         7906 g        11276 t
ORIGIN      
        1 aataagaaat atgtaaaata tacaggaaga tttggaatta atagaagaga acaatttaga
       61 ttaaaagaaa tcgttaagag agaaagtaaa aaaataacta tggccagtaa agaatcttta
      121 aaaaatgaaa aagaaaagat tctattttta ggtactgaag aatttatgta tatacctatg
      181 ctgtttgcaa aagaaaaaga aaatcaatat gatatatatt atcattcaac aacaagaagt
      241 ccaattatta atattgataa gaataattat cctattaaaa gtaaatttat gctaaagagt
      301 ttttataata tagatattca aaattatatt tacaatatag ataaacataa tttttctaag
      361 tgttatttat ttgtagagct gaataaaagt gaaaattctt atctagaatt tattgatata
      421 attaaaaaaa caagtataaa agaattaact atagtatgtt gtagctaaag tgttttaaca
      481 ggataaaata tatcacgatt ttaagatagg agttgagtca tatgagagat tttagcacgt
      541 attctaaaga tgatgttatt tttttattaa aagatatatc aaatttaatt gaagaagaag
      601 gaaataaaga aagagaatta aaaatacaaa atggtagaca ttattcagaa atgataccaa
      661 tagagtatga agttagtgaa gattatctga atttatatca tgagaaacta agagaaaata
      721 aagataaact tgcattttct ataggagtta tgtgtgaaaa aataattaaa aaaaatggaa
      781 atgatgtagt actagtttca cttgctagag caggaacacc tattggtatt ttggcaaaaa
      841 gatatataag aagcaagtat aatataagtt tacctcacta tacaatctct ataataagag
      901 ataagggaat agatatgaat gcaattgaat atatagtaaa gaatcatccg agctcaaaaa
      961 ttcaattttt agatggatgg actggtaagg gaactatatc aaaagaactt gaaaaagcat
     1021 gtgatgaact taatgttaaa tttaaaaaat attttgactc aacacttgct gtattagctg
     1081 accctgctgg atattcagga ctttatggaa ttagagaaga tttcttaata ccaagtgcat
     1141 gtttaaattc aaccgtttct ggtttagtaa gtagaactgt actaagagag gatttaattg
     1201 gtaaaaatga cttccatgga gctaagtttt atagacattt aaaagataaa gatgaatcta
     1261 tgaactatat cgaaactata gaagaatgtt ttaaaaatca atttaagaat atatctgatg
     1321 aagttgaaaa ctgggaaagt gacattataa caagagatgg atactttgat gttttaaata
     1381 taaaagaaaa gtataacata acagatatta actttatcaa accaggtgta ggggaaacga
     1441 caagggtttt acttagaaga gttccgtata agattcttgt taaagactta aatgataaat
     1501 ctttagacca tatattcata ttagctaagg agaaaaatgt tgaagttgaa caaatggatt
     1561 taaaagccta taaatgttgt ggaataataa aaaatatgaa agatatttag gaaatatatt
     1621 agacaggaga atattgaatg attgtttttt cagatttaga caggagtatc atttactcca
     1681 ataaattttt aaatgctgat agtaaatatg ctaatataga aatttatagg gaaaaagaaa
     1741 tatcttatat atctttagat actataaatt taataaagca aatacaatat tatggtatgt
     1801 ttatacctac aactactaga acagtggagc aatttaagcg tatagaattt aataaatatg
     1861 ggatttattt tccttggagt ataacttcaa atggaggagt tatacttaaa gataatgaaa
     1921 tattgaaatc atggagtgaa aaaatagata aactaaaatc tgattatgag cccattgaaa
     1981 gtatgattca taaatttaaa gactatttga atgtagatgg aataactaat tttaaagttg
     2041 cagaagatac atttttttat atagttgttg atttatcaag atttaatttg gattcaatca
     2101 aagaatatac aaatatattg gaatcaaaaa attggaagtt ttatgtgagt ggcagaaaaa
     2161 tatattttat accaaaagaa atttcaaaag aaaatgctat aaaatacttg actaaagaat
     2221 taggaataga atatttttat gcagttggag attctattat ggactatggt atgcttaata
     2281 tctcaaataa atcctatgta ttgaagcatg gtgatataaa taaaaatgaa atagaaaatt
     2341 catttatatc aagttctttc aatggaatga gtggaactga agagatacta tctaacattt
     2401 tagacgagaa ctgcctactt aatatataat gactgatata taaataataa actagaaaaa
     2461 tctgaaataa attatagtaa agtttatcat actaatttat ttcagatttt tatcaattta
     2521 tatttaagta agaactcaat atgttaaaat gaaggtggag tcattgccca gactgagata
     2581 catacctcat catcataatt ttcatacaca tgtggtatag tactgttaaa ataaaaacta
     2641 tctccttcct ccaatagata ttccttattt ccaagtgtta tcttcatttt accttttatt
     2701 atatagccac attcttctcc ttcgtgtgtc acaaattcac tgtttttttg tgtatgacca
     2761 tctagcgtaa caagtataaa ttctattttt ttcccagata aattaggaac aagaagttca
     2821 taaaattttg tagaatcatt agttactatt ttttttcgat tatttttctt tactactata
     2881 tcagagtcct ctcttccaat ctcttcaaaa aaataaccta tatttatatc aagagctttt
     2941 gatattttcc acattgctac aatagatgga acaaccatat tacgctcaat ttgacttatt
     3001 aagcctgtac ttaaatctga attttttgct aaagtagaga tacttatatt tttagatttt
     3061 ctaagagatc taattttgct tccaacatct atattcatta gatacaaacc ccctcagaca
     3121 tgattataac atataattta taaagattaa taaaataaaa aaaaagtttt acaatacaag
     3181 tattttttat gctatattga attttctgaa aagatgcatt ataatagtat taatattcaa
     3241 tatattgaat attgataacg atattttaat ttaatgaaat ggaggtaatt aagatgaaga
     3301 gttttgtaag tcctgataat atttgggtat tatgggcagt tgtaactgga tgggcagcat
     3361 ttagtattta tttggaacaa aagtacaatt gggcatcaaa agtgtcagga gcaataatag
     3421 ctcttgtagg agcaatgtta ttatcaaatc taaatattat accagtagag tctgttgtat
     3481 atgaccaagt atggggatat gttgttcctt tagccatagc tttattgtta tatcaatgta
     3541 acattaaaaa gatatggaaa gaaagtggaa gattgctcat tatatttctt gtaggttctg
     3601 taggaactgt acttggagct atgattggat ttttagcatt aaaaaatgta gtaccagatt
     3661 taaatattgt tgcagctatg atgacaggtt cttatatagg tggaaatgta aactttgctg
     3721 ctatgtcagg ggcgtttgat gcgccaggag aattagtatc agcaacagtt gtagcagata
     3781 atcttttaat ggcattatat ttctttgtct taatagctat accatcaatt ggatttttta
     3841 gaaaacattt taaacatcca catgtagatg aaatggaaag tataggtata actgaaggag
     3901 aaacagtagc agcaaattat tggggaagaa aagaaatatc tttaaaagat atagcaatag
     3961 ccattggtag cgcttttata attgtcgcag tatctgtaga actatcaact tggtttaaag
     4021 ctataatacc attatcaaat ccttttttag caatgttaaa tactttattt ggaaatcaat
     4081 atctaataat aactactttg actatgttgt ttgccacatt tatgcctaat ttctttggtg
     4141 atataaaagg agcacaggaa ttgggtacat ttttaatata tatattcttt gttgtagtag
     4201 gtgttcctgc ttcaataaca ttaataattc aaaaatcacc attactactt ttatactgcg
     4261 gaataatggt actaataaac atgctagtta catttatagt tgcaaaaata tttaagttta
     4321 gtttagaaga agctattctt gcatctaatg caaatattgg tggtcctaca acagcagcag
     4381 ctatggcaat atcaaaagga tggtctaagc tagtagggcc aatacttata gttggaacat
     4441 ttggatacat agttggtaat tattttggtc ttttagtagg aaatattttg atataatttt
     4501 acgtgaaaaa aagttgtttt ttagaaagat ttttatttat tagaaagatt tttatttaat
     4561 tggaggttgt tatgattttc gatggacatt cagatacttg ggcagatgta acaataagaa
     4621 ggcttaacgg ggaaaaagat gtatttagaa gaatccatct tgataatttt aaaagagcaa
     4681 atatagaagg agctattttt gtaatatgga tagacccacc atatgataaa gagcctgaaa
     4741 aaagagttaa acaaattata agctctataa aagatgaaat tgaagatatg aaagatgttg
     4801 taaacatagt aaaaaaatat tcagatatag ataaaggtat aagtgaaaac aaaataaata
     4861 ttttaattgg tcttgaagga ttaagccata ttggaagaaa tatagattta ctagattatt
     4921 tttataaaga agttggtgca agacatgcat cattaacttg gaatgaacag aatgaacttg
     4981 ctacaggatg tttaggggat gaaaatcgtg gtttaactga aataggtaaa aaagcagtca
     5041 ataaaatgga aaaattgggc atgatagtag atgtgtcaca tttaaatgaa aaatcatttt
     5101 gggatttaat gtctgttgca agtaagccag ttatagcttc tcattctaat gctaggagtt
     5161 tatgtgatgc taaaagaaat cttacagata atcaattaaa agaaatttca aatacaggtg
     5221 gattagttgg aataaatagt tttagaggat ttactaatag taatcctaag aagcaagatt
     5281 taatacattt ggttgaccat atagactata tggtaaatct tataggtata gaacatgtat
     5341 cttttggttt tgattttagt gattacttag ggaaagagac tataggttct tttactggaa
     5401 atgctagtgt gccaagtgta gatggtcttg aaagcacaca tacagcacag aacatagtag
     5461 tagagcttga aaatagaggt tataaaaaga aagatataga gttaatctct agtggtaatt
     5521 tttataggct ctttaaagat atactaaaat aatataataa agaacttaaa tttataaaat
     5581 agtatattta tacaaataat atctacatat ttaaattggc gttcataatt tattaaatta
     5641 aattttaatg ttactagaag tttcaattct agtaacattt ttttgactaa aaacacaata
     5701 aaaatctgat tatccgctaa aacacagtat ctatcagcat tttcaaggaa ataaacacat
     5761 caatttacta cttatttact actaacgaat gagccttgat acgcaagttt tcctagatat
     5821 gcaaagatat ggtacagcac atcagtattg aaacaagaaa aaattgaata acttatagac
     5881 tatggaacgc ctaacatgac gttccttttc tattttcagc cgttcttatt ggacggctat
     5941 tttattaccc gaatgaaagg agcattagat ttatgaaaaa gacattgaaa cgattgtgta
     6001 cgggcttctt agctcttgca actgtcgtta ctgctttacc gactacaccc gttcatgcag
     6061 aaagtaagca atactggacg gagtcaaaag agcgtgtcgg tatcgttgaa aaagtaatga
     6121 atgacggttc gattggttct acctttaatg agggacattt aactgttgag ggcgaggacg
     6181 cttactgtat tgacattaac acagacttta agaatggtta caagaccaga gctgacgcaa
     6241 gctcacgcat gagtatcgac cagataagcg acgtttcctt atctatcgaa tatgtaaaac
     6301 agtacacaga cagacacagc ggaataagca aaaatcacgc ttacctttta agacagcttg
     6361 tagtctggca gagattgagc gtacatcttg gctggcaatg tgataacgtg cgagcttctt
     6421 atgacgaaat accaaaggct acgcaggacg aagttttcgc tggtgcaaaa gcctttgtca
     6481 aagaaaataa aggacgctat gaatgtggcg gttatatcta ctctggcgag gggcaggaat
     6541 tgggacaatt ttgggcgaag ctgaatgccg gaaatgcgaa actgcaaaag acttccagta
     6601 ataccagcat taccgatggt aacgggaatt actctatcgc tggtgcgacc tacggtgtct
     6661 ttgctgataa ggactgcacg aaacagcttg ccacccttac gactgataaa aacggaaata
     6721 cagacgttgc agaggtaacg gctggcacag tttatatcaa ggaattatcc gcaccagcag
     6781 gatataaagt agataaaact gtatattctt taaaggttga agctggaaag acagcgacct
     6841 tgaatgtatc tgatacacca aaagtaacag acactttgat tgagcttttc aagattgata
     6901 tggaaacaca gaaagacaat ccgcagggga acgcttctct agcaggtgcg gaatttacat
     6961 ggaagtatta tgcaggcttc tatactaaag acaatcttcc tgccgaaact actcgtacat
     7021 gggttacaaa gacaatcgct gaaacagaca gcgacgggac aacccactac atcacaaaat
     7081 tagcggacgc atacaaggta tctggcgaca gcttctatat gcaggacggt aaagcggttc
     7141 ttccacttgg gacactaacc gttgaagaaa caaaagctcc aaacggctac ttgttagacg
     7201 gtgcatatat gcaggctggc gataagtccg aacagattaa gggcttatat gtaacacaga
     7261 ttaccgagga cggcgacctt gccgtactat ctggaagtaa ccagttttcc gtatcagaca
     7321 aggttatccg tggcggtgtc aaaattcaga aacgagattt agaaacgggc gatacaaagc
     7381 cacaaggaag tgccactttg aaagatactg cctttgacat catttcctta aatgataatt
     7441 ctgttttagt tgagggaaag ctatacaaga aaaatgaagt ggtaaagaca attcgtacag
     7501 atattgaggg tgtcgcttct acttctgctg accttttacc ttatggaaaa ttccgtatcg
     7561 ttgaaagtga agctcccgac ggttacttaa ctgacggtgc aaaaacgatt gattttacaa
     7621 tcacagaaaa tggaaaaatc gtggacttaa ccgacgaagc ccattctatc tacaatcaga
     7681 ttaagcgagg ggatattgag ggtgtaaaaa tcggtgcagg cacacacaag cgtcttgctg
     7741 atgttccgtt taggatcaca agcaagacga cgggcgaaaa tcatgtagtg gtaactgatg
     7801 ataacgggca gttctccact tctgctgaat gggcttcaca taagcataat accaacgcag
     7861 gcaagaccag cgaggacggt gtgtggtttg ggacttctga accagacgac agcaagggtg
     7921 cgttacctta tgatacctac atcatcgagg aattacgctc tgaaagcaac aaaggctttg
     7981 aacttatccc accttttgaa atcgtggtat caagaaataa ccttgtgatt gatttaggaa
     8041 cactgactga tgaatacgaa aaagaaattt ctatccatac cacagcgacc agcaaggacg
     8101 gcgaaaagac tatccttgca ggaaaagagg ttacaatcgt tgatactgta aaattagacg
     8161 gacttacaaa aggtacaaag tatcagttga aaggctggca gatgttaaaa gaagaaaatg
     8221 ccgagcttat cattgacggg aaacgtgtag aaaacgatta tacctttgtc gctgatgatg
     8281 aggaaatgaa agtggaaatt tcctatacat tcaatgcgtc tgctttaggt ggcaaaaccc
     8341 ttgttacctt tgaagaattg tatgatttaa gcaatccaga tgaacccgtg aaagttgcag
     8401 aacataagga cattgaggac gacgggcaaa cggtacttat cacagagcgt atcatcaaga
     8461 tacatactac cgctaccgat aaggacggca acaaagagat tgaagctgaa aaagacgtta
     8521 caatcattga taccgtaacc ttagagggct tagaagtcgg tacacagtac aaacttgtgg
     8581 gctggcagat gttgaaagaa gaaaatgcag agcttcttat caatggaaag cgtgtggaaa
     8641 gtgattatat gtttaccgct gacagcgaaa ctatgaaagt ggaagttgcc tttacttttg
     8701 acgctacttc tcttaacgga aaacagcttg taacttttga agaattgtac gatttaagca
     8761 atccagatga gccgaagaaa gttaccgagc ataaggatat tgaggataag ggacagacga
     8821 ttacttttaa ggaaaagcca gaagaaccag aaaaaccaga aacaccaccg acaccagaaa
     8881 agcctagcag acctagcgac agtcccaaaa cgggcgataa caccaacctt tacggactgc
     8941 ttgcacttct tgtgacttct ggtgctgggc tggcaggtat cttcttctat aaacgccgta
     9001 aaatgaagaa atcataaggc ggtaacggta tgatgaaaga aaaatcacgc tctccgccga
     9061 agctgtcaaa cggcagactt ttgcttattc tggcttgtct gctacttacc tgcaagaata
     9121 cggatagtca agggtgcgta agcattgatt ttagccttta ctatctgggg gattgctggt
     9181 acttcccaaa ccgccagaat aagaaatcac aatcaaaaaa cttatcaaaa atagaaagaa
     9241 acgaggtaaa acactatgga aatgaaatat gtcgtgccag atatggcaca gtcttttgga
     9301 actcttgaat ttgcaggcga aagcgagcca gtctttgaaa gagataaaaa taaccgcagg
     9361 gtcttagcca gacgaagcta taatctttat tctgacgtac agaaaggcga aaatgttgtg
     9421 gtagaaattc ccgtgcaggc tggcgaaaag catttcaagt atgagcagaa agtaaaactt
     9481 gtcaatccga agttatacgg cagaggttac tcaatcgggg atatgggaca taccgattat
     9541 gtgttagttg ctgacgatat tgtagcagtt gaagaaaagt aaaggagtga agaatttatg
     9601 agattatcaa acggatttgt tattgacaaa gagaaaacat ttggagaatt aaaatttaca
     9661 gcggtgcgtg atgtgttctt gcaaaatgag gacgggacac cgagtagcca gcttaaaaag
     9721 cgtatctatg acttgaagtg cagtctgcac ggtggaatta tccccgtgtc tgtaccgcca
     9781 gaagtaccgt taagggaatt tccgtacaat gcagtcgtgg agcttgtcaa tcccgtagcc
     9841 gatacagtat cacgaaagac ctatacgggt gcagatgtgg actggtatgt aaaggcagag
     9901 gatattgtgt tgaagaataa aggcaatcag aacgcaggca gtccacagaa ccatacaccg
     9961 cagggacagc agaaaaaata ggcttgctga atatattttc attgtagcgg atatatggaa
    10021 ctgttataat atggatatga agaaagcgac aaattagaat ttatgaggtg gatataatgt
    10081 ttgaagattt ggataagttt atagcagaaa attcagagga aattcttgtt actgctgggc
    10141 gaacagatga taaaatatct gaattggaaa cacagttgga tattgcattt agagaagaag
    10201 ttaagatatt tatacggaaa tatggagttt tgattggata tggtgttgaa attgtagcgt
    10261 gtggtcttaa tggacattct cgatttgttg aagatacact tcgctttaga aaaaatggtt
    10321 tggaaaagaa atatattgtc atcgaaggag atagtgagtt ggcgtattgt ttgaacaacg
    10381 aaacgggtga gattgtcaac tggggattag acaattttga agtgtatctt gtagccgaaa
    10441 gtttagaatc ttttgtattg gcacaatttg aagaaggtaa gacaaattgg tgataaaatc
    10501 ccagtttgtt taactagaaa gaaatagaaa aattagaagt tgtagtacaa aaataggagc
    10561 atgaaacatg aacataaaag aagcaataaa aatgatacag gacgagaaat tacaaggtta
    10621 taatatgaat gaaaggaaag atgatagaga aaatgaagtt gtgttaatgc gtgagaatga
    10681 taagtggatt gtttatgcaa cagatgaacg ggctagtaag gttacaaatt cacaagatat
    10741 ttatttagat gaggaagaag ccttagatga ttttattgat agattaagag cttttaatag
    10801 attaaggtag ctatgggaga attaaaatga cctctaaaaa gatatttagt aattttcttt
    10861 taaatagaca actcccagtt cgtttaacta aaaagaaata gaaaaattag aattgtatgt
    10921 ggaggaatgc gaagtgaaaa tatattacaa aggcttttta tgcaatcttg ccccctatcg
    10981 tgtgatgggc gaagatagac atgcattatt tcctgttacc cagtcgaatg atcctatatt
    11041 ttatgaagaa tttgatgaag ttcactatgg attatgggcg aaagtattaa cagatgaaga
    11101 atatcaagaa atcgttgata ctgttacgaa aaatgaatag acaacttaga atttattaaa
    11161 aaggttggag aagaaaatga ttaaaagaat atggacattt aaaaggatta cattagcaat
    11221 aattacattt atgtgtacta taatttttgc aatctcattg caaaaaagta ttatgggaac
    11281 agataaatta caaagagatt ttggagtcgc atttcttttt attgttgtgc ttgtttgttt
    11341 cctttacttt ttatatagac tattaatacc taaaagtttt aggtgtatga cagtagtaaa
    11401 aaaatattta tcttttagag agttgaaaga ccgaattaat aatgaatcat tttcaaaagt
    11461 cgttattgac gagaagaaat ctggaaagat tgaaatatat tattcatcta aatggattta
    11521 tgctgatgaa gtttatatac caagaaaatt agttttggat ttgatagaag aacgcaaatc
    11581 tttatattca tcgtttgaaa aattatcaat agcaacaaaa aatggagaaa atataatatt
    11641 tgccatagtt gatattgaag aagcagaaaa aatcataaat tctttgcaag aaatttttga
    11701 agagtttaca ttggatttta ataatatgag aaaaattcaa aatagacttt taagaaaaga
    11761 aataaagcaa gaattttata aacgagttat aaataaagaa gactttttga aagaaagcgg
    11821 tttgtagagc atgttaaaat aggaacaata gttgcaataa tattaggttt attcatattt
    11881 acgtatggaa ttgatttgtt attaaaactt ttgactatgt tattgggtcg ataatatatt
    11941 tcttgtttgc gaattgtctg acaacttcat atcacacata aagcagttag tcttaggatt
    12001 gactgctttt ttgtacccag aaaggagtga ttgacttatg cataagattt ggaataaagg
    12061 acaccgtatc agagccagcg acaagcacct tgtctaccac ttttccatag ggacgctttt
    12121 gtttgtattc gtggcggttc tcctgctact gaatatcaaa cagctcatgc gtaccgattg
    12181 ggagcatttc agattgttag agaacggctt gacgctctcc ccttacaact tcataaccat
    12241 actgatagcg actggtgttt gtgcattggt cgcttttctg tattaccgtt tttgttatga
    12301 cagtttcaag aagctcctgc accgacaaaa gctggcgaga atgatactgg aaaataagtg
    12361 gtatgaagcc gataccgtac aagacagcgg tttttttact gacctgcaaa gcagatcaag
    12421 ggaaaaaatc gtctggtttc caaagattta ttatcaaatg gaaaagggac tgcttcatat
    12481 ccgctgtgaa atcacgctgg ggaaatatca agaccagctt ttacggttag aggataaatt
    12541 ggaaagtggc ttgtattgcg agctgaccga caagacactg catgacggct atatcgaata
    12601 taccctgctt tatgatatga tagcgaaccg cattaccatt gatgaagtac gggcagaaaa
    12661 cggctgtctt agactgatga aaaatcttgt ctgggaatat gacgcactcc cacacgctct
    12721 gattgctggt ggtacgggtg gcggtaaaac ttattttctg ctgacactca ttgaagcctt
    12781 actgcatacc aacgctgtcc tttatatctt agacccgaag aacgctgacc ttgcggactt
    12841 aggaacagtt atgggaaatg tgtatcacac caaagaagaa atgatagatt gcgtcaatgc
    12901 cttttatgag ggtatggtac agcgaagtga ggaaatgaag cgacacccga actataagac
    12961 gggcgaaaac tatgcctatc tgggacttcc accctgcttt cttatctttg atgaatatgt
    13021 ggcgtttttt gaaatgctgg ggacgaaaga aagcgtgagc ttacttagcc agttaaagaa
    13081 aatcgttatg ttaggacggc aagcaggtta tttccttatc gttgcctgcc agcgtccaga
    13141 cgcaaagtat ttttcagacg gtatcagaga taacttcaat ttccgtgtgg gacttggacg
    13201 tatcagcgaa ttaggttacg gtatgctgtt cggttcagat gtgaaaaaac agttttttca
    13261 gaagcgtatc aaggggcgtg gctattgtga tgtgggaaca agtgttataa gtgaatttta
    13321 cacgccttta gttccgaaag gacatgattt tctacaagaa atcggcaggc tggaagaaaa
    13381 aaggactgca agcaacgaaa gctgacagtc ctacttcaat ttcaatatgt catttgggga
    13441 acattccaga aaatcacaga gctttgcaag gattgtgagg tcaattctgg aaaccttgtt
    13501 cttgcaatag ttattaagct gtgtccgctg taatttacac gcttcacaga ctttgttttt
    13561 actgatattc ttctctttca aaagattttc taaatcaata tatacctgca tacgctgtac
    13621 ctcgtctttt ctttgaatta taagatacaa ctgtattgat aaataagatg tattaccgta
    13681 gactgtatta taatacaccc tacaagaaag gacagtaaca gtatgtgcaa aaagagaaaa
    13741 atcataacaa tcacggtact ttttgtgctg ggagtggcgg tttcctatct gcaatgggga
    13801 cgtgaaattg atgtagcagg aagtatgggt acttcatcag aaaatttcca tgaagaacac
    13861 gttaccttta cgcttaaccg tcttatcata acagacaaag aaaagtgtgc agaggaaatc
    13921 atcaaaaaat gcagggagaa tgattttact tctatcctgt tcagctatga tgtggcaaag
    13981 ccaaacgctc tatatggtac ggtgtaccgt tcccgttttt ccatgaaagg acaaaagcct
    14041 ttgtttcact tccgatatac acagacagcg gacacgctcg gttcatacaa cattgttgat
    14101 gatccagaag cgttcacgct ggtaatcgaa taacgcaagg caggacggga cggcgacgtg
    14161 cgaagcgaaa ggcgacggca cggactagcc gttgctggtg tggcataagc cacgccagca
    14221 ggtaaaaccc ctcggtatct aacagagggg tacgtttgca aatagacaat agacaaaaaa
    14281 cataggaaac ataaggcttc tggcatgatt tcctagcaaa attcagttgt gaagtcgcct
    14341 taaaaaactt cacactttac agattggggg tatggttctg aatgaagaac aatggataaa
    14401 agaattacgg gagaaacgga ttgcttacgg tatctcacaa ggcaggctgg cggtggcttc
    14461 tggtatcaca agggaatatc tcaacaagat agaaagcgga aaaatgaagc cgtcaaagga
    14521 acttctggaa actttgcata aagaactggc aaggttcaat ccagaagcac cgcttactat
    14581 gctgtttgat tatgtgaaaa ttcgtttttc cacgctggat atacagcaca tcatcaaaga
    14641 tatattaaaa ctgaatatca actatatgct ccatgaagat tacggacatt acagttatac
    14701 ggagcattat tctttagggg acatctttat ctatacgtcg gctgacgaag aaaaaggtgt
    14761 ccttttagag ttaaaggggc gtggttgcag gcagtttgaa agttacctgt tagcacaaca
    14821 aagaagctgg tatgactttc tcatggacgc actcgtagac ggtggcgtga tgaagcgtat
    14881 cgaccttgct atcaacgacc atacgggcat tttagatatt ccagagcttg cggaaaaatg
    14941 cagaaaacag gaatatattg gaaagtccag aagttacaag ttttaccagt cgggcgagct
    15001 tatcaagcac agagagcatg acagagaata tatgggacgt accctttatc ttggttcgct
    15061 gaaatcagat gtatatttct gtatctatga aaaggactat gagcagtatg tcaagttggg
    15121 aacacctctg gaagaagccg acattatcaa ccgttttgag atacggcttc gcaatgaacg
    15181 agcctattat gcagtacgag atttgctgac ctattatgac gcagagcaga ccgccttttc
    15241 tatcatcaac cagtatgtgc ggtttgttga tgaagaaccc gacaagcgaa aaaatgactg
    15301 gaaactcaat gaccgctggg cttggtttat tggcgataac agacagagct tgaagctgac
    15361 gacaaagcca gagccttaca ccttagaccg cacattgcgt tgggtacaaa ggcaggtagc
    15421 accgaccttg aaaatgctga aaaagattga taagggaaac ggtacagact acatggaaac
    15481 aatcgaacag caggcaaagc tcacagaaaa gcatgaaatg ataatcaaac agcagacgac
    15541 ccctgcaaaa gatttggtgg aaagttagga gtgataaaaa atgtgggtat tattaaaaga
    15601 cttcctgctg gtatctatgg gaatgggtat tggcgtagtc ttgatgtgta ttttgaatgt
    15661 tggcaaagaa gctgactatg aaatgaaaca attagaagaa agcgaggaca attaaatgaa
    15721 tttcggacaa aatttatatc aatggttttt atcaaacgca cagagcttag tgcttatggc
    15781 gattgtggtt atcggtatct acttagggtt caagcgtgag ttttcaaagc tcattggctt
    15841 cttggtagtt gccttgattg ctgtcggctt ggtattcaat gctggtggtg tgaaagatgt
    15901 actgttagag ctgttcaata agattatcgg tgcataaaat tttattgtgg tgctgatatg
    15961 agaatggtat aatgtggata tgaagaaaac ggcaaatttg aatttgtttg atgttagtaa
    16021 tacaagaatt tgaagtttca gaggtaaaga taattgatat cttcagataa agggaaatat
    16081 aattattaaa atagcaaaat caaaagaagt taaaataaat ttataaagga tggatgagaa
    16141 atgatgtatg gtattatcgc tgttgcactg atatttgtat ttgctaattt accccaatat
    16201 attagaaaac agctaacaga aaaaatcgct gatgaaagca gtaaagctaa agaaaattta
    16261 caaacttcac aattaaattt tttaacaaag atagaatggc ttaaattcta tccattgaaa
    16321 aaaattttac taggagtaat ttggcttgtt tgcctatttt atgctgtaag aggggtcttt
    16381 atgactatta acgatcctcg tggtgtattt atattcctca cagttttatt ttggcttatc
    16441 actattttta cagcacgcaa gatatggcga tatattaaat tatcttatca ctgcgtacct
    16501 gtattaaatc atatcaaaac aaaggaggaa ctaaaggaat ctctacaagg tgaatgtttt
    16561 gaaaaagtat tgtttgagca caatatatta cgaaaatatt tccctgtcct tattagtgaa
    16621 aactgggtag ttatgaacgg tcgtcttttt ccaagacacg gagttgaaaa aatatattat
    16681 ttacacgaaa gccctgtttg gaattatgaa caaattagat ttatatattc taatggtgaa
    16741 gaatacctaa ctcctagtga cagacaaact gtagatgaac ttcgtcaaac agaaatatcg
    16801 aatttattac ataaaatatg tccggtagtt attgaaaaag cagaagatat aaatactaca
    16861 tcaaataaaa aagataaatc aattatctat tggaaaatga attataaagg taaatttaga
    16921 agaacattat ggatgatacc atttgttatt atcttatgta ttttactgcc actatgctta
    16981 ggaagttctt ggattatata cgatataatt atgattgcaa ttttagtatg gcagctttgg
    17041 tatacttata aaaaaatgaa gcttgaagaa agaactttaa atgagataga tgaaaacaat
    17101 aacaaaaaat atacgatagc tatgaaaaac attcatatgt acaattatct tcttgcaata
    17161 aaagatgaaa cgcatatcta caaagccatc attgaagccg tacccgacga aaataatgac
    17221 atgatgattt ggttagatga tttcaatttt ccacaaacag agattgagga cataaaaaaa
    17281 gaaatagaac tgtactttaa agcaaaaagt attaattgca tatttaaagc tggtaaaaga
    17341 gtatagacga agtgttcaga cacggcggtg gaaaaatctc ttagactaca aaagtcaaga
    17401 gcaaaaattt cagtttgaca atttcatatc catatacaca aagcagttag tcttatgatt
    17461 gactgctttt ttcttaccaa aatttcagat tggagtgatg aaatgagcga tatttttaag
    17521 gatatgcagt caaaagtcgg ctgtgaatac atttccgacc tgccctctta caaacgtaag
    17581 gtgtggcagg aaatgaaacg gctgaaccct gccgattatg aagaaagaca gttagaagat
    17641 ttttcaaaat atgtgtttgg tatgtcgtac cagaccttaa aagatgtaat gaaacaacag
    17701 aaaggacgtg aggaacaatg caggaaacaa gggtgctggt ggaaacgaga ggaacaactg
    17761 gcgaagaaac aatatcatac tggttcgact tgccgataga tgttgccgaa tttgaagaaa
    17821 agttaggtat cggtgcagaa agtcaagatt atcgtattat cgaaaaggtg ttgccttatg
    17881 ctgatgaagt ccacgaacat acaagcgtgt atcagctcaa cgaattagat tttatgtacc
    17941 gccagctttc aagtgatatg caggaagaat atgtttcact acttacggtg tatgaaaatt
    18001 tagaagcact ttatatttgc aggaacgtga ttacggttta tcccgactgc aaaagcatga
    18061 ttgatgttgc taggcaaaag ctgatgaacg acccgacgtt caaacattta tccgaggact
    18121 gtcaagccta ctattttgac tttgaagcct atgcttctca cttgcaggaa cacgggaaat
    18181 ttttagtaac agaacacggt atctttgaac tgccagagta ggaaatgagg tggtgcttat
    18241 gattgatgat atggcggttt atattgctaa tcttggcaaa tacaacgagg gctatttagt
    18301 cggtgcttgg ttcacatttc ctattgacga ggaagatgta aaagaaaaaa tcggcttgaa
    18361 tgaacagtac gaggaatacg caatccatga taccgataac ttcccgattg cgattggcga
    18421 gtatgtttcc attgaagaac tcaatgagat gtatgaaatg atagaggaac ttcccgacta
    18481 tatcgtagaa tgtctggacg aatttatcag ccactacggg acgctggaag aagtcgtgga
    18541 acataaggac gatatttatt attatcccga ctgtgaaacc atgacagatg ttgcctacta
    18601 ctacatagac gaattgcagg cactagggga tattccaccc agcttacaga actacattga
    18661 ctatgaagcc tacgggcgag atttggatat gggcggttgc ttcattgaaa caagccgagg
    18721 tatgtgcgag ataccatatt aacgctggaa gatcagcgac aagcattgtt gctactgaca
    18781 gcgtatttct cttttaaggg acagtctgaa acatggctgt ccttttctat tttgaaagga
    18841 gctgaaagaa cttgaaaaag attaaaagct acacgggtat ctggaacgtg gaaaaagtct
    18901 tatatgcaat caatgacttt aacttaccct ttcccgttac ttttacacag attacatggt
    18961 ttgtgattac agaatttatc atcattctgt ttggggatat tcccccactt tccatgattg
    19021 agggagcatt tctcaaatac ttcggtattc ccgttgctct cacttggttt atgtcgcaga
    19081 aaacctttga cggaaagaag ccgtacagct ttttgaaatc acagataacc tatgccctgc
    19141 gacctaaaat cacttatgca ggaaaagccg taaaactgca taagcagata ttgaatgaaa
    19201 caatcacggc agtaaggagt gtgaacgatg ttcccgataa aatatattga caataacctt
    19261 gtctggaaca aggacaatga ggtatttgcc tattatgagc tgataccgta taactattct
    19321 tttctatccg cagagcagaa atttatcgtg catgacagct ttcgccagct tatcgcacag
    19381 tcccgtgagg gtaaaattca tgcgttgcag attgccacag aaagctctat tagaagtatg
    19441 caggagcagt caaaaaagct ggtaacgggg aaacttcgtg aggtggcagt tcagaagata
    19501 gacgaacaga ccgaagcgtt ggtatctatg attggggaca atcaagtgga ctaccgcttt
    19561 tttcttggct ttaagctcat ggttacggaa gaacagctca atctgaagaa catcaaaaaa
    19621 tcggcatggc tgacgttcac ggaatttctc catgaagtga accacacgct gatgaacgat
    19681 tttgtttcca tgccgaatga tgaaatcaac cgttacatga aaatggaaaa gttactggaa
    19741 aataaaatct cccgtcgctt taaggtgcgt cgcttggaaa tcaatgattt tgggtatctc
    19801 atggaacatc tttacggcag ggacggtgtc gcctatgaag attatgagta ccagctacca
    19861 aagaagaaat tgcagaaaga aacgctgata aaatactacg accttatccg tccgacaagg
    19921 tgtgtgattg aggaaagcca gcggtattta cgattggaac atgaggacaa ggaaagctat
    19981 gtgtcctact ttaccgtcaa tgcgattgta ggggagcttg attttccgtc ctctgaaatc
    20041 ttctatttcc agcaacagca attcacattc cccgttgata cttctatgaa tgtagaaatc
    20101 gtggaaaatc gaaaagcatt aacaaccgtc cgcaacaaga aaaaggaatt gaaagacctt
    20161 gacaatcacg cttatcaagc aggaagtgaa accagctcaa atgtggtgga cgcattagac
    20221 agcgtggacg agctggaaac agacttagac cagagcaaag aaagtatgta taagttaagc
    20281 tacgtgatac gggtgtctgc acccgatctt gacgagctga aacgccgttg tgatgaagtc
    20341 aaagactttt acgacgacct caatgtaaag ctggtgcgtc ctgctgggga tatgctgggg
    20401 cttcattctg aatttttacc agccagcaag cgatatatca atgactatgt gcagtatgta
    20461 aaatcagatt ttttagctgg gcttggtttt ggtgcgacac agcagttagg ggaaactacg
    20521 ggtatctata tgggctattc cgttgatacg ggaagaaatg tgtacctgca accgtcttta
    20581 gcttcgcagg gcgtaaaagg cacagttaca aatgcccttg cttctgcttt tgtcggttcg
    20641 cttggcggtg gtaagtcgtt ctgcaataat cttctggtat attatgcggt gctgtttggc
    20701 ggtcaagcac ttctgttaga ccctaaaagt gagcgtggca actggaaaga aacgctccca
    20761 gaaatcgccc atgaaatcaa tatcgtaaac ctaaccagcg ataaggacaa tgcaggactt
    20821 cttgacccgt ttgtgattat gaagaatgta aaagacgctg aaagtctggc aatcgacatc
    20881 ttaacattcc ttacgggtat ttcctctagg gacggcgaaa aattccccgt gttacgaaaa
    20941 gcggttcgtt ccgttaccca gagcgacagt cggggcttgc tccatgtgat agacgagcta
    21001 cgccgtgaag atacgcccat atcaagaaat attgcagacc atatcgacag cttcacggac
    21061 tacgactttg cacacctgct gttttcggac ggtacggtag aaaatgctat cagtctggat
    21121 aatcagctta atatcattca agtagcagac cttgtgttgc cagataaaga taccactttt
    21181 gaggaataca cgaccattga attattgtcg gtgtctatgc tgattgtgat tagtaccttt
    21241 gcccttgatt ttatccattc ggacagaagc atttttaaga ttgtagacct tgacgaagcg
    21301 tgggcgttct taaatgtggc acaaggcgaa accttatcaa ataagctggt tcgtgctgga
    21361 cgagctatgc aggcaggcgt ttattttgtt acccagtccg cctatgatgt ttcaaaggaa
    21421 agtctgaaaa acaatatcgg cttgaagttt gcctttcgta gtaccgacat caacgagata
    21481 aagcagacct tagagttttt cggtattgac aaagacgacg aaaacaacca gaaacggctt
    21541 cgtgatttgg agaacggaca atgcttattg caggacttat acgggcgtgt cggtgtggtg
    21601 cagatacacc cagtctttga agaactgcta cacgcctttg ataccagacc gcccgtacag
    21661 agaaatgagg tggagtgatg aaagaaagga taaaaggtgc gttcacaaaa aagaagattt
    21721 tccactttct caaaatggct ctgttcgtgg tggcactctc ccttatcctg ctttcacttc
    21781 tggggacggt ggctcatgcg acggggcttg tggacgatac cataaatgca gaaaatcttt
    21841 attcaaaata tcccctttcc aactaccagc ttgactttta tgtagataat agctggtcgt
    21901 ggcttccgtg gaactggctg gacgggattg gaaaatcggt gcagtacgga ctttactgta
    21961 ttaccaactt tgtctggacg ataagccttt acttatctaa cgcaactggg tatgtggtgc
    22021 aggaagccta taaacttgat tttattaacg atatggcaga cagtatcggg cagagcatac
    22081 agacccttgc aggtgtaacc gagaacggct tttcttccac gggcttttat gtcggtttcc
    22141 tgcttctcat tatcttagtg gtgggaatgt atgttgctta tacgggactg ataaaacggg
    22201 aaacaagcaa ggcacttcac gctgttatca actttgtggt ggtgttcgtg ctgtccgctt
    22261 cctttattgc ctacgctccc gactacatca agaagataaa tgagttttca tcagacatca
    22321 gtaccgcttc acttgacttg ggaacaaaaa tcatgctccc taactctgac agcgagggca
    22381 aggacagcgt agacttgata cgggacagct tattttctat tcaagtggaa cagccgtggc
    22441 tacttctgca attcggtaac agcaatgcag aggaaatcgg gacagaccgt gtagaagctc
    22501 tggtatcggc aagcccagag gacgaggacg ggaaaaccag agaagaagtc gtaaaagcag
    22561 aaatcgagga caacgacaac aacaatctga caataccgca ggtggtaaac cgcttgggta
    22621 tggtgttttt cctactgttc ttcaacttgg ggataacaat atttgtattc ttgcttacgg
    22681 gcatgatgtt gttcagccag atacttttta ttatctttgc aatgttttta cctatcagtt
    22741 ttctactttc catgataccg agctatgaaa gcatggcaaa gcaggcaatc gtgagagtgt
    22801 ttaataccat aatgacacgg gcaggaatta cgcttattgt aacggtggcg ttcagcattt
    22861 ccagtatgtt ttataacatt tctacggatt atccgttttt catggtggcg ttcttgcaga
    22921 tagtatgctt cgctggtatc tatatgaagc tgggcgattt aatgagtatg ttctctttga
    22981 acgctaatga cagtcaaagc atgggacgaa gaattttccg cagaccgtat ctgtttatgc
    23041 gacatagggc taggcgtatg gaacaccgta ttgcaagggc ggtaagtgct ggcggtattt
    23101 cgggcggtgt ggctggtgcg gtagctggaa gtgccgttgc tggaaaacga gctgaaagaa
    23161 aaaatacagc ttctaaagaa aatcgaggca ataccacttc cagcatggga cagcgtgcag
    23221 gctcaaaggt tggtgctgtc ttagatacga aaaataaagt aaaagacaag gcaaacgctg
    23281 tcaaagaaaa catcaaggat atgccaacac agaccgctta tgcggtatat tccgcaaagg
    23341 aaaaagcaaa gtccagcgtg tctgacttca agcgtggcat ggtgcaggaa cagcagtcca
    23401 gacaaacggg acgattggaa aagcagacac agcatagaca aaatatcgcc gataagcgta
    23461 tggagcttca aaaggcacaa gaagcaaggc aggcacagcg aaaggctgac ggatcagcga
    23521 caacgggagc tacccgtccc catgagcgac cagccacggc ttcaaccatt ccaaagccga
    23581 gtatggaaaa aacgcaggaa gtcaaacgcc ctgccacagc gaccacttca aaaacaagtg
    23641 agccagtcaa gacaagtgtt atcaaagagc gtccgctatc ttctggtgct tctgatagaa
    23701 aagcgaccca gtctgtacag ccagtacata ggcagaatgt agaaaaagtg gtatcgcagg
    23761 aaacacgcca gaactacaaa gcagagcgta ctacaaagac acagaccttt gaacaatcaa
    23821 agcgtacaac agaacatacc aaaaagaacc gtaaccttgt aacgaagaaa ggacagaaga
    23881 aaaaatgaaa ctgaaacata tcgctattat cggcagtctg tttcctatcc tcttttctct
    23941 ggtacttttc tttggtgtcc tcattagtgc ggacagcgac gacgagaaca gcaatttttc
    24001 ttctggcatt acgggtatga acttatccgc agaagtcttg aaacatcagc ctatggtaga
    24061 aaaatacgcc agagaaaacg gtatttccga gtatgtcaat gtgctacttg ctatcattca
    24121 agtagaaagt ggcggtacgg cagaagatgt tatgcagagt tcggaaagtc tgggactacc
    24181 gcccaactct ttagatacga aaagctcaat caagcagggg tgtaaatatt ttgcgtccct
    24241 gctttcttcc tgcaaaaatc aaggtatcga cgacttgaat gtagcgatac agtcctataa
    24301 ctatggcggt ggctatgtgg gatatgtggc aggaaaagga aagaaacaca cctttaacct
    24361 tgcagagagt ttcgcccgtg agaagtcggg tggaaagaaa gtaacctata cgaacccgat
    24421 agccgttgcg aagaacgggg gctggcggta tggctatgga aatatgttct acgtcgaatt
    24481 agtcaatcaa tatttgactg tggcacattt tgataatgcg acggcacaag cgattatgaa
    24541 tgaagcgttg aaatatcaag gctggaaata tgtgtatggt ggcagtaacc cgaacacttc
    24601 ctttgattgt agcggacttg tgcaatggtg ttatggaaaa gctggtatct ccttaccgag
    24661 aaccgcacaa gcacaatatg acgctaccca acatcttcca ctctcgcagg caaaggctgg
    24721 ggacttggta tttttccatt ccacttataa cgctggaagt tatgtaacac acgtcggtat
    24781 tcttgtttcg ccgacacaga tgtatcatgc aggcgacccg ataggatatg cagacctaag
    24841 tagtagttac tggcaacagc acttaatcgg tgcaggacga gtaaaacaat agaaaggact
    24901 tgaaaatatg tttaagaaga ataagaaaca aacagaaact atcaaagaac caaaagaaaa
    24961 aaaggtgcgt actgttaagg taggcacaca taagaaaacc gtgattgcgt tgtgggtggt
    25021 gcttatcgca agcgtgagct ttggagtgta taagaatttt actgctatcg accagcacac
    25081 cacccatgaa aaagaaatca ttgagcttcg cttgcaggac accaacggga ttgaaaattt
    25141 cgtgaagaac tttgcgaagt cctactacac atggaataac agcaaagaag cgattgaagc
    25201 aaggacgcag gcaatcagcg gttatctgac aaaggaattg caggacttga atgttgatac
    25261 aatcaaaacg gatataccaa ccagctccac ggttacagat gtgattgtat ggcatatcga
    25321 gcagtcggga acggacactt tttctgctac ctacgaagta gatcagcaga taaaagaggg
    25381 agaacagaca agcaatgtga aagcaaccta taccgtgaaa gtccatgtgg acgctgacgg
    25441 ggacatggta atcgttcaga accctaccct tgccccagca atcgaaaaat cggactatga
    25501 accaaagaca ccagaagccg acaacagcgt ggacgctgac acagtgaatg acgctacggc
    25561 atttctggaa actttcttta agctgtaccc gacagccaca gaaaaggaac ttgcctacta
    25621 tgtagcagga aatgtgcttg aacctatcgg cagggactgc ctttattctg aactggtaaa
    25681 ccctatcttc acaaaggacg gggacaatgt gaaagtcaag tttgccgtga aatcccttga
    25741 caatcagaca aaggcaacac aggtatcgca atatgagctt gtgttacata aggacgggaa
    25801 ttggaaaatt ataggataat caagagtgtt catttattga cttattttct tttgtgcggt
    25861 atatttttat cgtaatgtcg gaaatatacc gcattttgag gtggacaatg attaagaaca
    25921 gaattaaaga atttagagca aggtatgata tgaaacaaga ggacttggca aaagctgtcg
    25981 gtgttcgacg ggaaacgatt ggtaatcttg aaaaaggacg atacaatccc tctctcgtat
    26041 tggcttggaa tattgcaaag acgtttcatg taacgattga agaagttttc acagtaatag
    26101 acgattgaat ttaggagtga taccatgtta ggactaaaaa aacgagatat taagaaagcc
    26161 ttgaaagcag gtgcaaaagc tggtggtgtt tcattggctg atgtacgagc tgatattgaa
    26221 gcgacgattg atgaagctat gaatagtacc gacccagaag tgcaggcaaa tttcaaaaag
    26281 tattttggaa ataaacgccc tacaccagaa gaatatattt ataaaattac aaaaaagaca
    26341 aaagtttaat gttgaaaaac cttaacgaaa attaagctat gataaaatct atgcagagag
    26401 gagttataac atgggaagtg ttttgattat agacgatgat aaagaattat gtacgttaat
    26461 gaaaaaatgt atagagcaag aagaaatgtc agctttggtg gcaaatggtg gtacagaggg
    26521 tttacgtttg atagatgaaa ataaaaattt attttctctt gttattttag atgtaatgat
    26581 gcctgatata gacgggtttc aagttttaga aaaaattagg gaaacaagta atattcccgt
    26641 tctaatgctc acagcaaaaa gtggtgagga cgataaggtt tttggactgc gtctaggagc
    26701 agacgattat ttgacaaagc cttttagtat taaggaactt atggctcgtg tcaattcact
    26761 tatccgacgt tacacgactt taaatcctac atttgcagaa gaagattgta taactttaaa
    26821 aggcatgaaa attgataaag tgaaccgtac tgttttagtt aataatattt ctgttgaact
    26881 tacaagtaag gaatttgatt tacttacatt tcttgcttct aacaagggac gaatatttac
    26941 aaaaaagcag atttatacac aagtatggaa agacgactat gcttttgatg ataacaatat
    27001 aatgtctttc atcagcaaat taaggaaaaa gattgaagca gacccagaac acccctttta
    27061 cattttgacg atacgagggg ttggctaccg ctttaacaag gaggcttaat tatgaatact
    27121 tatctgctta ttgcctttgt tttagcactt gtaataatcg cttgtcttgt ttataaactt
    27181 tatcatatta acgagcaaat ttcttttatc aaagaagtgc ttgttgacat aaaatctggt
    27241 aaccttaaca gacgtattct agtacaagaa aacgatataa ctaaacagat ttgttatgat
    27301 ataaatcaaa tagcaatcaa taatcaatca cagctcattc atcaaaagca atcggaacaa
    27361 gcgtacaaac gactaatgac aagcctttca catgatgtga aaactccact tgcttcttta
    27421 gtcggttatt tagaagcggt tgaaagtcaa attgtaatcg gcaaagaaaa agacgaatat
    27481 attcatgttg cgtctgaaaa ggcacaacat cttaaacatt ttgtagaaca cttatttgaa
    27541 tgggtaaaat tagactctgg ggagcagatt tttcattttg aaattttgga tttgaacgaa
    27601 ttgactcgta atattatgac tgattggatt ttaatattgg aaaatagtta ttttgaatat
    27661 gaggttgaga taccagaaga agaatatcaa gtgcgaattg atataaacgc atacactcgt
    27721 attatcaaca atcttttaca aaatataatt gttcatagtg agggaaacaa aatgatgttg
    27781 cgagttctga aagatcagca acgaattaaa gttattataa cagataatgg aaaaggaatt
    27841 gcttctgata aattgccaca tatatttgaa aggttgtatc aatgcgataa ttctcgctct
    27901 accaaaggaa atggtctagg attggcaatt acaaaggaac ttgtcaacgc acataaaggg
    27961 acaattactg caaatagtat tctaaatcat ggaacagaat ttatcctatc atttccaaag
    28021 gctctgtaaa aatgcagagc tttctttttg tgaaagcaag ataaaaacaa ggttacggca
    28081 aggttttggc aaggttcata tactataatt taggtgtaag tggtaattct aataaattgc
    28141 cacttagata gaacagtaaa tagaaagtga ggaaatttgt atgagtgata ctgtgataaa
    28201 gacaaagcaa ttaaccaaaa agtacggaaa acaaaccgta gtaaataagg ttaatcttaa
    28261 tgtaaagaaa ggtaggattt atggcttgct gggacgtaac ggagctggga aaacaaccat
    28321 aatgaaaatg atattaggat tgacaataac tacttctgga aatgtagaag tttttggaaa
    28381 aaatatagac ggaaatgaga aaaggatata ttccagaatt ggtgcaatta tagaaacacc
    28441 ggggttttat cccaatttga ctggaacaga aaatttagaa attttcgcta agttacgagg
    28501 aacagcttcg cctaatgctg ttaaacacgc attagaagtt gtgggtctgc cctataaaga
    28561 caaaaagacg tttgcgaaat actctcttgg tatgaaacaa cgtttaggta ttgcaaatgc
    28621 gatattacat gaccctgaat tgttaatttt ggacgagcca acaaatgggc ttgacccgat
    28681 tgggattgca gaagtacgag atttcataaa gaaactcagt gtagaacacg gaaaaacaat
    28741 tttaatttct tctcatattc tttctgaaat agcagtgctt gcagatgata taggaattat
    28801 tgataaaggt gttcttttag aagaaagtag tatgagtgag ttagaaaagc gaaataggaa
    28861 atatatttcg ttgcaagttt cggacattcc aaaaacaata ctgatattag aacgtcaatt
    28921 tcatgtgaaa gattattcag ttcaagatga acagacggtg caaatttatg acacgacatt
    28981 gaatatggga gaagttaata aagctcttgt catggaagat ataacagtta tcagttctgg
    29041 actttgtaac gataccttag aagattattt caaaaagatt acagggggtg agggtattgc
    29101 ttaagctggt taaacttgaa ttttgtaagt tgcgtcgtaa aaaacttatt tggttgatgt
    29161 tactatctgc ttttgtcatg ccattctttg catttttata ttttggttat ttgggacaga
    29221 caaatattga tcctagacta ttttataagt ggtctgcatt tggatgtaca ttattcatta
    29281 tactgccatt tatattaggc ttatttagca caatgcttat gcatgatgaa aataggtatg
    29341 atatgctaaa acaactttgg attgtgccag taagtaaaat gggatatttt ttcagtaaat
    29401 ttttcgttgt attgatttat tccgtttgtt ttatgcttat tacagcattt gcttctgtac
    29461 tgtttagtgt cattccgaac tatgtagtat ttgaatggag cagtgttttg tttttgttgg
    29521 aaaggtgtct ggaaattgct gttttgacat catttgcaat actacccatt ttagcaatat
    29581 cttcttcaca aaaaggatat attttccctc tttgcattac attgatttat gtatttgctg
    29641 gatttttaat cacaccgata aattcttgtc tacacccact gtcttgtatc agcgttatta
    29701 ttgcaagaaa tgaagcaatt ttaggattgt cacttccaca aaataatgtg ccattagcac
    29761 ttttaagcat ttgtgtatgg ggaattgctt ctactttact tgctaaaatc aaattaagca
    29821 cgagaaagta aggtggaaaa atgagaatac ttttatgggc agaatttcaa aaactccgcc
    29881 gttccaatat tattatgttt actatctttt caacaatctt aatcgctgta attgttttga
    29941 taagcggggt gacaacagta tctgaagaac agttaacaac agctatggct ggctggtata
    30001 tgacaattac acaagtctgg gcgactatgt tcgttcttcc agccgtaatt gctcttttgg
    30061 ggagttatat gatttgtcgt gaggaacaag acgatacatt aaaatcttta cgtttgattc
    30121 cagtaaatga aacaaaattg acaactgcaa aaatgattgt tgcattggta tttagtatgc
    30181 tggtgtattt gttacttttt atgattacat ttgtaacaga agctattttg catttctctg
    30241 atttatctat ggcaatgttt agtgaatttt taaaaatgta tttattagag gggattggcg
    30301 tgttttttgc cgtttccccg attattgcaa tcgtaccata tctaaagaaa agttattggt
    30361 tggctctact tttagcagaa atctattcat ttgctggatt atttatgagt atgtcgaata
    30421 ccctgaaaac tttttatcct atcacagcaa tatttggtat ttcggggtat tacgaaacaa
    30481 ctattcagga ttatttttgt agtttgggaa tattgatttt ttgcggttgt atctcagcta
    30541 tcttattaaa aaatttaaac catagacaaa ggaaaaaggc taatgaataa actatgtaga
    30601 ataagaaaag ggaaaatctt ttttggggtt tgtggtggtt tagcaaagtg ttttaatgta
    30661 gacgttaaaa tagtaaggtt actatttact ataacgggtt ttatttttcc caaaagcatt
    30721 attgcatata tcattcttat gattgttata ccgaaagaac caaaaacacg ttaaattata
    30781 ggttattgta acttacggat tgtcggctct tacttgctac ctgcggagca gatcagcaaa
    30841 caaaaaacta gtagaacgct actaaaaaga aaaataggga aaacaagaag ctgattactt
    30901 cttgctttcc ctgctattca agataccgtc aatcgtacct tgaatgattt ttaattcgtt
    30961 atcactcaat aagtcaagtg atgtttctat ctgacggcga gctgtacttt tctcaacttt
    31021 ctctgatgga tagaaatatt catcaaccga tatattgaac atggtaacta aatcatggaa
    31081 taattgaaaa cttgggtgtg aaccgatatt ttcaatatct gcgatatgtc gttctccata
    31141 gaatacctta tcgcctaaat cgttacggga tagccctgct ttttcacggg cttctctgat
    31201 tgccagtcct aatggtctga aatcaaaggt ttgtgtttct ttattttttc tcattttaaa
    31261 ccacctagca tgagtttaaa cttcatgcca tgtatttaga atgtgttaaa agtgcatata
    31321 gtatgcagta ataattcgaa aggtattatc gtgtgcggta aataaaaaaa accgttctta
    31381 gataatatgc ttttgcacgt cttaaaaacg ataacgggac ggttttggat ataaccaaag
    31441 ctgtcctttt cttttggtat agctctggtt tgagggggct atgccatgat tacatataga
    31501 agaacgctac gtcgttctca actggataat tatttctgtc tagtgcttca atcatcatac
    31561 ccattgcatg agttaaaaat tcatatagat agcatttata taacagtctg tttgtgtagt
    31621 tacattcaaa tgggctgttt ttattttata gaaataattt tttgaaaatc ttggtagttt
    31681 gccacctctt tgggaagtta gggtgcggaa agggggaaat gaacaaaagt tcatttcaat
    31741 ccagcacaga aaggaggtga gcaaatgaaa acatcttctt tcaaacaagc cattgaagca
    31801 cagtttgatt gtctttcaaa aaaggttatc aaacgagctg tcaaaaaagg ctatcgtgat
    31861 atgaagcgac gtgaaaaacg agaatgtcca ttttctgata ttccagatta tgagcaggaa
    31921 cgctttggaa catttgataa atacgaaagt gattacaccg tatttaatat tttaggcatt
    31981 gaagtatggg ttgaggacga ccagttaagc gaagctctta aaacattgac agaaaagaaa
    32041 cgcaacatta ttttgttgtc ctactttatg gatatgtctg atagtgaaat cagcgaattt
    32101 atcaaaatcc cacgttctaa cgtccagtat catagaacaa aaacactcga aacaatgaaa
    32161 aaaatcatgg aggaacgtaa atgaagaaac atgagcattt gccttttgag gtaatcttat
    32221 cagccacaga gggcgaacca gaagcagttg aaaccgtttt gaagttttat gacggttaca
    32281 tttccaaatt atgcttgcgt aaattgtatg acgaatacgg taatgtttgc atggttgtgg
    32341 acgctgattt aaaaaacaga gttcagacag cattacttga tatgctgatg aattttgaaa
    32401 ttgtagtgat gtagtacaca taagtgaagt ttggtacttc cccctttcct gccacttcac
    32461 ttgtcattct attaaaatca atttgactag aattgatttt gataggctga atagccgata
    32521 gttctttgaa aattgaataa agcagacaga tacgtttgtg aggtagcaag ccacggggac
    32581 tgtacgccac gacctttcca aaagaaagcg agcgacccac gcagtgatcc gagagcgacc
    32641 gttggaaaag tcgctttgcc acgacctacc ctcacagaat aatgatacgt ccgcatggtg
    32701 cggttctgcc cacaagaatg ggaatagttg agatactaac ggagctttcc aaagccgtct
    32761 gtctgcttgt aaaaaatgac acacagtaaa gggggtgcat agattatgaa caatactgat
    32821 gtgcctatct gggaaaaata tacgctgacg attgaagaag cgtccaaata cttccgtatt
    32881 ggcgaaaaga aattgcgtaa attagctgaa gaaaatcttg acgctggctg ggttatcgtg
    32941 aacggtaatc gtattcagat aaagcgaaaa caatttgaaa aaatcataga tacattggac
    33001 gaaatctaat gtcattgagc cgtgaatatg gtataataag gtatagcgta tcacggctca
    33061 ttccatgacg gaaaggagct ttacactatg tctaatgtaa aacgaaaaga cagtaaaaat
    33121 cgcaatttgc gtaatggaga gagccagcga aaagacggaa gatacgttta taaatatacc
    33181 gatatatacg gaaagccaca atttatctat tcttggaaac ttgtaccgac agataagaca
    33241 cctgctggaa agcgtgatga tatatcgttg agggaaaaag aagcacagat aaaaaaagac
    33301 cttaacgatg gtatcgacac agtcggcggt aaaatgacag tctgccagct ctacgagaag
    33361 aagaacagcc aaagaaagaa catcaagagg gctactgaaa aaggacgatt gtatcttatg
    33421 aacgctctga aaaatgaccc gttgggtatg agggcgattg ataccgtcaa acagtcggac
    33481 gctaaagaat gggctatcag aatgagtgaa aaaggatatg cctataaaac gattgataac
    33541 tataagcgtt ccttgaaagc gtcattttac atggcgatac aagacgactg tattagaaag
    33601 aacccgtttg agtttaagct aagtgatgtt ctggaagatg ataccgaaca gaaagttatc
    33661 cttacaccag agcaggaaga acgcctgctt gcttttatgg aaacggacaa gatttacagt
    33721 aagtattacg atgaggttgt gcttctgctg gaaacgggac ttcgcatttc tgaattttgc
    33781 ggattgacga cacatattga tatgcagaac agaatactca atatagacca ccagttattg
    33841 aaagatagcg aaatcggtta ctatattgaa acgccaaaga ccaaaaacgg aaaacggcaa
    33901 cttccattaa cagaacgggc ttatcaagca atccaaagaa tattaaagaa cagagaaaag
    33961 gcacaaccgc tgattgtagg tggttacagc aatttcctat tcttaaaccg tgagggcttg
    34021 cctaaagttg caggaaacta tgagggcatg gtgcgaggac taattaagaa gtataacaag
    34081 tatcacacgg ataagttacc gaacatcaca ccacattcat tccgacatac ttattgtacg
    34141 aatatggcaa acaaaggaat gaacccaaac actctgcaat atcttatggg acacgctaac
    34201 ataactatga cacttggcta ttacgcacac ggtacatttc aatctgcaaa agcagaactg
    34261 gaaagactgg cttgttaata tcggagctta tatttactac tcatttacta cttttggttg
    34321 cattttcatg ctggtaaatg ccagcttatg caaggtatct tccaaaaaga aaataccgat
    34381 aaagccctaa aatacgggat atatcggcat ttaccaattt atgaaaagat aatcagaaat
    34441 ttagtaagtt ttttgactaa aaacagatat agatatgtat tgacaatgat aattattatt
    34501 gataaactat aaaagtgaaa atgataataa ttttcatttt aattaataaa agatttttaa
    34561 ggagggagaa tgaagaaaag gtatttagtc ataggaataa taattttgtc ttatttttct
    34621 ttatttatag gagctgaaga tataaatata atgcatatat ttgcaaaaaa tcagcataaa
    34681 cttatgatat ttatcatgag tagaatacca agattaataa gtatattgat tgctggtgta
    34741 ggaatgagca tagcaggtct tataatgcaa cagataagta agaataaatt tgtatcacct
    34801 acaacaggtg ctacaataga tgcagcacaa tttgggatag taatatgtat gcttttagta
    34861 ccaactgcct ctatttttac aaagacaata atagcatttg tattttctct tgtagggact
    34921 tttacgttta tgaaaataat agggaagtta caatttaaaa atataatctt tgtaccatta
    34981 gtaggtataa tgtttggaaa tataataggt tctatgacgg attttatcgc gtataaatac
    35041 gatttgagtc aaaatgttag ttcttggatg caaggtgact tttctatgat acttaaagga
    35101 aattatgaga tattatatat cacaataccc cttataatat tagcatatat ttatgcaaat
    35161 aaatttactg ttgttggtat gggtatggat tttgcgacaa atttaggtct tagttacaaa
    35221 agaattgtca atataggtct gataatagta gcacttgtta cagtatgtgt agtcgttaca
    35281 gctggtaata tcccttttat aggtctaata gttccaaata tcgtatcctt gtacatgggt
    35341 gataatataa gagcaagcat atggtatact ggtttacttg gagcgatatt tgtacttata
    35401 tgtgatattt ttggaagaat tattatttat ccatatgaaa tatctatagg gcttacagtt
    35461 ggagttatag gaagtatact attcctttat ttaatattaa ggaggaacgt aaatgaagct
    35521 tagtctcaat caaaaaattt actttgtaag tatattacta ttcatatcaa tagcactttt
    35581 tgtaatttat ggaatagaca tgaatcactt agaatatgca ttatcacaaa gaataccaaa
    35641 aatatttgct atggtattag gtggaggatg tatagcattt actactgtag tgtttcaaac
    35701 aattacaaac aatcaaatat taacaccaag tgtactaggt ttagattcat tgtatgtaat
    35761 gattcaaact ataatagtat tcatatttgg ttcaagcagt agtctgatta taaatgaaaa
    35821 ttataatttt attattaatg tagctatgat gattggagct tcattgttat tatataagac
    35881 gttatttgaa aaaaataaaa ataatatttt ctttttaatc ctagtaggaa tgatgtttgg
    35941 aaccttattt aaaagtgcaa caacatttat tcaggtaatg atagacccga atgaattttt
    36001 agctttacaa actagtataa tggcaagttt aaacaatata aacactaatg tattgttaat
    36061 agcatttata ataattattg ccataatacc ttttatatat gatgaaataa agtatttaga
    36121 tgtattgtcg ttaggtaaag aacaagcaat taatttaggt gtagattttg ataaagcagt
    36181 taaaaaaatg ataatattaa tagcaatttt agtctcaata tcaacagcac ttattggacc
    36241 tatgacattt ttaggacttt tacttgctaa tataacaaga gaaattttta aaacatataa
    36301 gcatacatac ttgatttcgg gttctatgtt gatagggatg attacattga taataggtca
    36361 attttttata caacatgtat ttaagtttga tacaacttta agtgtagtaa taaattttat
    36421 aggaggaatt tactttattc aattactatt aaagggggca aatagatgat agagataaaa
    36481 aatatattta aaagatataa gaataaaaat gttgtagatg atgtctcttt tagtatagaa
    36541 aagggaaaga taacatcttt tataggacca aatggagctg gaaaaagtac agtactatca
    36601 atagttacaa gacttatagg aggtgatggt ggagaagtaa taatagaagg aaaaagcttg
    36661 actaattata gcaataaaga attagcaaaa aaaatagcta ttcttaaaca atctaacaat
    36721 ataactttaa agcttacaat aagagaatta gttggatttg gaagatttcc atatagtgaa
    36781 ggtaatctaa ctaaagagga tgaaaattat attgatgaag caatagaata tatgaagttg
    36841 actgatatac aacataagta tttagatgaa ttgagtggag gtcaaagaca aagagcttat
    36901 atagcaatgg taatagctca agatacagag tacatattac ttgatgaacc actgaacaat
    36961 ttggatatga accattccat acagatgatg aaggtactta gaagtctttg tgatgagtta
    37021 gataagacta tagtattagt aatgcatgat ataaattttg catcatgtta ttcagataat
    37081 atagttgcat taaaaaatgg aaaagttaaa aaagtaggtc gtacagatga gatagtaaat
    37141 gaaaaagttt tagaagatat ttatgagatg aattttaaca ttaagaatat aaatggaaat
    37201 cgaatctgta tttattttta aaatattgta tatgaaaata aacgtgctat atgatttttt
    37261 ataaagtttt taaattaaaa taaagattaa taggagagag gaaaatattg atatgaataa
    37321 aaaagcagca atagtagcag cagtagctat tataggatta gtgacagtgt ttgcattagg
    37381 tggaagtaaa aagaatgaat caaaaacatc agaaaattca aataatacta tgaagataac
    37441 tcataacttg ggagaaacag atgtaaaatt aaatcctaaa aaagtagtgg tatttgatta
    37501 ttcggcttta gatacaatgg atgcattagg tgtagctgag aatttagtag gacttccaaa
    37561 agcgagttta ccagcatcct tagaaaaata taaggatgac aagtatgcag atttaggagg
    37621 attaaaagaa ccagatttag agggtataaa gtcggcaaat ccagatttaa ttataataaa
    37681 tggaagacaa gaagattttt atgagcaatt gtcaaaaata gcaccaacaa taagtacaag
    37741 taaagatgat aagaagtact tagaatctgt taaaaataat atagacaaaa tagcaaaaat
    37801 atttggagta gaagaaaaag caaatcaaga atttagtaaa attgaaaaga aaatagaaac
    37861 tttgaataaa aaagtaacag ataaaaattt aaatgcttta actataatgg taaatgaagg
    37921 taatttaagt gtatttggtg aagaatcaag atttagtata ttatacaata gttttggatt
    37981 tgaaaataaa gataaaaata taaaagaatc aagtcatgga caaaatatta cttttgaata
    38041 tatagctaaa caaaatccag aagttatgtt cgttatagat agaggaatag ctactggcag
    38101 tgatgtaaaa gaaagctcaa ctgcaaaatc tgttttgaac aatgatataa taaaatctat
    38161 ggatgcatat aagaatgata atataatata tttagattca ccaacatggt atgtaaatga
    38221 tggtggtcta acatctttaa ataaaatgat agatgatgct tcaaaagcgg taaattaata
    38281 atccgatata accaaaatgt attttttata aaatgattat caaataaact aggagttttt
    38341 aagaactcct agtttatttt ttgcataaaa aagatgtagt ttaaacaata atattatcta
    38401 taataagata aatctattca aaatctaata aaatatgaag ttatactaga caaaaag
//
LOCUS       DAFKMX010000014        37314 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFKMX010000014 DAFKMX010000000
VERSION     DAFKMX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.014"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(227..802)
                     /locus_tag="LZB08_000600"
     CDS             complement(227..802)
                     /locus_tag="LZB08_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBM7314119.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            1062..2354
                     /locus_tag="LZB08_000601"
     CDS             1062..2354
                     /locus_tag="LZB08_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBM7314120.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            2459..3061
                     /gene="yedF"
                     /locus_tag="LZB08_000602"
     CDS             2459..3061
                     /gene="yedF"
                     /locus_tag="LZB08_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBM7314121.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            3134..3397
                     /locus_tag="LZB08_000603"
     CDS             3134..3397
                     /locus_tag="LZB08_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBM7314122.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            3412..3597
                     /locus_tag="LZB08_000604"
     CDS             3412..3597
                     /locus_tag="LZB08_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBM7314123.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            3700..4896
                     /locus_tag="LZB08_000605"
     CDS             3700..4896
                     /locus_tag="LZB08_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7314124.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            5110..5388
                     /locus_tag="LZB08_000606"
     CDS             5110..5388
                     /locus_tag="LZB08_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBM7314125.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            5425..5859
                     /gene="ssb"
                     /locus_tag="LZB08_000607"
     CDS             5425..5859
                     /gene="ssb"
                     /locus_tag="LZB08_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBM7314126.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            5878..6105
                     /locus_tag="LZB08_000608"
     CDS             5878..6105
                     /locus_tag="LZB08_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBM7314127.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            6248..6568
                     /locus_tag="LZB08_000609"
     CDS             6248..6568
                     /locus_tag="LZB08_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBM7314128.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            6576..7532
                     /locus_tag="LZB08_000610"
     CDS             6576..7532
                     /locus_tag="LZB08_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBM7314129.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            7543..9537
                     /locus_tag="LZB08_000611"
     CDS             7543..9537
                     /locus_tag="LZB08_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBM7314130.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTAHIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            9538..9987
                     /locus_tag="LZB08_000612"
     CDS             9538..9987
                     /locus_tag="LZB08_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBM7314131.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            10006..11334
                     /gene="dnaB"
                     /locus_tag="LZB08_000613"
     CDS             10006..11334
                     /gene="dnaB"
                     /locus_tag="LZB08_000613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBM7314132.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            11539..11847
                     /locus_tag="LZB08_000614"
     CDS             11539..11847
                     /locus_tag="LZB08_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314133.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            11929..13218
                     /locus_tag="LZB08_000615"
     CDS             11929..13218
                     /locus_tag="LZB08_000615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBM7314134.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            13544..13619
                     /locus_tag="LZB08_000616"
     tRNA            13544..13619
                     /locus_tag="LZB08_000616"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13577..13579,aa:Lys,seq:ttt)
     gene            13626..13700
                     /locus_tag="LZB08_000617"
     tRNA            13626..13700
                     /locus_tag="LZB08_000617"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13659..13661,aa:Glu,seq:ttc)
     gene            13705..13780
                     /locus_tag="LZB08_000618"
     tRNA            13705..13780
                     /locus_tag="LZB08_000618"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13738..13740,aa:Val,seq:tac)
     gene            13814..13888
                     /locus_tag="LZB08_000619"
     tRNA            13814..13888
                     /locus_tag="LZB08_000619"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13846..13848,aa:Thr,seq:tgt)
     gene            complement(14271..15263)
                     /locus_tag="LZB08_000620"
     CDS             complement(14271..15263)
                     /locus_tag="LZB08_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314135.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(15272..16330)
                     /locus_tag="LZB08_000621"
     CDS             complement(15272..16330)
                     /locus_tag="LZB08_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBM7314136.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            16455..17918
                     /locus_tag="LZB08_000622"
     CDS             16455..17918
                     /locus_tag="LZB08_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBM7314137.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            18020..18817
                     /locus_tag="LZB08_000623"
     CDS             18020..18817
                     /locus_tag="LZB08_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7314138.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            18830..20932
                     /locus_tag="LZB08_000624"
     CDS             18830..20932
                     /locus_tag="LZB08_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBM7314139.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            21000..21479
                     /gene="rlmH"
                     /locus_tag="LZB08_000625"
     CDS             21000..21479
                     /gene="rlmH"
                     /locus_tag="LZB08_000625"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBM7314140.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            21546..23237
                     /locus_tag="LZB08_000626"
     CDS             21546..23237
                     /locus_tag="LZB08_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314141.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRSAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCLVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            23469..24941
                     /locus_tag="LZB08_000627"
     CDS             23469..24941
                     /locus_tag="LZB08_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314142.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVNQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEVFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDSVYGIPQHLLLEVIKNGKEFFTK"
     gene            25171..25980
                     /locus_tag="LZB08_000628"
     CDS             25171..25980
                     /locus_tag="LZB08_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314143.1"
                     /translation="MGDIHIVDDSLCKYSKTVLVLSNQEFAKVLKDQIISCIDDNIKI
                     LSNYSFAMLILPMNLLYGNDMKIIHEYSNNIFLNFFSRKDLNLDTYFEEFNSIEDIAL
                     ALNPGIESYIVFDEGEDLTKNLEERFKSFIDKGKVPLPAHKSDAFKFWLIIYGFITQA
                     LDIILSCKGFNLTPYLRYRVTLQYVLLLGDNVFDIEDLSNMLLKCKVAHVLYAIFDKS
                     EIKNISFVQFYKKLSEYKFSYKVEDDIKENININQIAEILNKNITLFLNYI"
     gene            26120..27277
                     /locus_tag="LZB08_000629"
     CDS             26120..27277
                     /locus_tag="LZB08_000629"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7314144.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFIDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCEIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIAKLNNYIDRLKKYTKDLHIMGERNSYSKTDNDATFMRM
                     KKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKFKYPKI
                     VADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKNDYYIC
                     KNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLENRTKNL
                     QVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
     gene            27719..28030
                     /locus_tag="LZB08_000630"
     CDS             27719..28030
                     /locus_tag="LZB08_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314145.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            28078..28395
                     /locus_tag="LZB08_000631"
     CDS             28078..28395
                     /locus_tag="LZB08_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7314146.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            28608..29309
                     /locus_tag="LZB08_000632"
     CDS             28608..29309
                     /locus_tag="LZB08_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7314147.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            29533..30834
                     /locus_tag="LZB08_000633"
     CDS             29533..30834
                     /locus_tag="LZB08_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7314148.1"
                     /translation="MENFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            30971..32065
                     /locus_tag="LZB08_000634"
     CDS             30971..32065
                     /locus_tag="LZB08_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBM7314149.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            32067..33200
                     /locus_tag="LZB08_000635"
     CDS             32067..33200
                     /locus_tag="LZB08_000635"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBM7314150.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDVGKFY
                     EDTKRYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            33218..34327
                     /locus_tag="LZB08_000636"
     CDS             33218..34327
                     /locus_tag="LZB08_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBM7314151.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            34392..35132
                     /locus_tag="LZB08_000637"
     CDS             34392..35132
                     /locus_tag="LZB08_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBM7314152.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKIKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            35137..36129
                     /locus_tag="LZB08_000638"
     CDS             35137..36129
                     /locus_tag="LZB08_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBM7314153.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(36317..37198)
                     /locus_tag="LZB08_000639"
     CDS             complement(36317..37198)
                     /locus_tag="LZB08_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7314154.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFIRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
BASE COUNT        14844 a         3904 c         6471 g        12095 t
ORIGIN      
        1 gacaatctag taaataagat aaagataaca ttagtaggat aaaatattgg tatataaaag
       61 atctaaatag aacctgaaag gtgttgtttc aaaattgatt ttttataatc aaatttttat
      121 ttctattttt atgtttttgt atgtaaaaaa gaaaggaagg agataattca aatttttatt
      181 ttgaaccatc tccttctata tgataattta taattgtttt aagaagttac tttaagtaat
      241 ctccttcttc aagaactata tgaccacttt cgtaattaga accatcaaca gtaattttta
      301 tttgttcaac attcatattt tttatgtaag tacgagcaac tgaatcaagc attaaagttt
      361 ctgcatcact acctaagttt gactttgtaa agtcagagct aacatctaat ataccagttt
      421 ttacaccatc aatgtctttt gtagtaaatg aatttaattt tgcagattct ggaacaactt
      481 taagtttttg aagttcttca aataaagtgt tctcatcaat tttatttaaa tcaactttat
      541 tttcaattaa atctgtctta tctacatcaa aagagtaaat agtagctgat tgagttttag
      601 tttcttcttt tggagtaggt ttagctttgt tattaggaac ctctttttca gtattgtttt
      661 cttcttttgg ttgctcaaca tttgtattgc tcttagtttc ttttttagat gaatcacttc
      721 cattttgagc attagtacaa ccaactgcga atatagatat agataatata cttaacacta
      781 acattatttt cttatttttc ataaaaattt ctcctctctt aaaaactatt cttatatata
      841 attatagtcg ttatgagcaa atatatgaat acaaaatagt aacaaattta aaaaatgatt
      901 tatttaaatt aataaaactc aaaataaact aaattttagt catttttatg ggaaatcatt
      961 taatgtacag tgaaaatatt gtaaaataaa agtagtgttt ttaaaataaa tataagttat
     1021 aattatatta taaaaaaatg tcttcaaaag gtgggaatag tatggaaatt ttgagcttag
     1081 gagaaaagat taaaaaatta agaaaagaaa aaaacatgac attaaaagaa ttagctggag
     1141 ataggataac agcagcacaa ataagccata tagagaggga taaatctcat actagttatg
     1201 aactgttgga atatctaggc gataagctag atgtaagtgt agaatactta ttagagacca
     1261 aggaaatgca atcaaaaaag ataactgata atttaatact acagagcgaa atttatataa
     1321 aagaaaatga attaaacaag gctgaggacc aaataaaaga agttttagat atatgtaaga
     1381 gatacgattt aatggagaac tatggcagat gtaatttttt aatggctaat attaatttga
     1441 aaaaatctaa ttataatgat gctatagaaa actttgaaaa ggcattgtac ttctttataa
     1501 aaaatagtga tagtgaaaat atacttaagt gttatttaaa catagggaaa atatatatgc
     1561 aggaagactt ttataaaggt gctataagcc actttaattt tgcagaggaa attttgtcag
     1621 agaataaatt tgaggatgtt aacatacaca aggaactata tagtaagatg gcatattgtt
     1681 acataaaact agataatcca aatatgtcat tgaagtacat agaaaagata aatgaaatag
     1741 ataatagaaa tgatagaaaa gaagatgtcg atattttggt gttaaaagca aataatatgc
     1801 ttgagattgg aaaatatgaa gaatcaagag attactttaa aaaagcacta aaaatacttg
     1861 agagtgagga taataaaact ggattagcaa atgtgtattt aacaatttgt gatgtttaca
     1921 gaaaaatagg tgaaacagat agagtacttg aatattcaca aaaggtatat gacataaaga
     1981 aaaatgatga agatgaatac atgatgtcag gattatttaa gataatagaa gcatatatag
     2041 ataaagaaga ttatgattta gctagaaaat attgtaagat agccttagct tcatcaataa
     2101 aaaataaaaa taaattcaat gaatataaag cattgagatt ctatgctaat atgtataaaa
     2161 atcagaatga agtaactctt gcaattgaat atctgattaa atgcataaaa atagtatcag
     2221 atttaggcaa caatagaata ttggccaatt tatatataga cttaggtcaa ttatattcaa
     2281 atatatcaaa agaaaaagag ttagaatatt atcaaaaagg tgtatttatg tacaaaaatt
     2341 tagaaataat gtagggtaaa taaacctata agggtataga aacaatctat aacaagcaag
     2401 ataatatcta tgataaaata taaattgtat gaatattttt aaggagatga atcagacaat
     2461 gagtataaaa atagatgcga gagggcttgc ttgtcctaaa ccagttataa atacaaaaag
     2521 agaattggat aacctagaag aaggtgttgt agttacaata gttgataatg aaacagctaa
     2581 ggagaatata ttaaaacttg ctaaatcttt aagttgtgaa gcaaatattg ttgacgaaaa
     2641 agagaatttt atatctatag aaattagaaa aggtaaaaat atactagata aacaaaatgt
     2701 tgtagataat aaagaaagtg aattagacaa tacatgtata tttatatctt cagataagat
     2761 gggacttgga aatgacgaat taggaaaagt gttaataaaa ggatttatat atacattaac
     2821 agaatctaaa ccttatccta agtatgtact tttagttaat ggaggagtaa aattaagtgc
     2881 tgaaaatgaa gaaaccatag aaaatctaaa aatacttgag aatatgggtg tagaagtttt
     2941 atcttgtggt acttgtttag attattataa tttaaaagac aaattacaag taggtacagt
     3001 tacaaatatg tatactatag ttgagacatt aaagaatgca tcaaatacca tttctatata
     3061 atgtatgtat gtttaataat ataaaactaa caataaagat taactcaaat agatagtcag
     3121 aaaggattaa gtaatgaatc aaatgtatat agtgtcattt aattcgacac atcatgcgat
     3181 aagatcagaa aagttatttg gtgaaaatag tctaaaagta atggctcttc caacaccaag
     3241 agaaataaca gcaagttgtg gaatatcaat taaattttct tttgaagata tggaaaagat
     3301 aaaaactatt ttggttgaaa ataatgtaga tataaaagga atttattgta taagtaaatt
     3361 agaaaatggt tcaaaagaag tagagaagtt agattaggag gctaattatt tatgccaatg
     3421 gatttaaaaa taggtgatat tgttgaatta aaaaagcaac atgcatgtgg gtgtaaagag
     3481 tttgaaatca taagaacggg aatggatata aagataaaat gtactaaatg ttcaaggcta
     3541 ataatgcttg atagagagac cttagaaaag agagtaaaaa agctaataaa aaagtgataa
     3601 aaatttaaca cttaaaaaat gtcaaatacc gtatattaat atccaaaatt ttggtataat
     3661 aagaattgaa gaaaacattt tcatgggggt gctttaatta tggcagttaa atatgcaaaa
     3721 agaatgcaag gattacaagg atcagaaata cgtgagcttt taaaacttac tcaacaacca
     3781 caaataatat cttttgctgg tggaatgccg gctccagagt tatttccagt tgaggaaatg
     3841 aaaaaagtat cagttgcagt gcttgaagaa aatggaagat cagctatgca atatactaca
     3901 acagaaggat atgaaccatt aagagaaaaa atagcagcta gaatgaatga taaaaacaaa
     3961 actaatgtaa acaaagatga tatattagtt acaagtggtt ctcaacaagg tctagatttt
     4021 gcaggaaaag tattcattga tgagggtgat gtaattttat gtgaaagtcc atcttacata
     4081 ggagctataa acgcatttaa atcttatcaa cctaaattca tagatgttcc aacagattct
     4141 gatggaatga taatggaaga attagaaaag atactagaaa caactgatag aataaaaatg
     4201 atttacgtaa tacctgactt ccaaaatcca acaggaagaa cttggccact tgaaagacgt
     4261 aaaaaattca tggaaatagt aaataaattt gaaataccag taatagaaga taatccatat
     4321 ggagatttaa gatttgaagg agagacttta ccatctttaa aatcaatgga tacaaaagga
     4381 ttagtaatat tcttaggaac tttctctaaa atattctgcc caggatacag attaggatgg
     4441 acttgtgctt ctccagaaat tttatctaag tttaactttg caaaacaagg tgcagactta
     4501 caagcatcaa ctatatctca aatggaagta agcaaattta tggatatgta tgacctagat
     4561 gcacatgttg acaaaataaa agcagtttat gttaaacgta gagatgtaat gcttaagact
     4621 atggaagaag agttcccaga aggattagta tttactcatc cagaaggtgg attatttaca
     4681 tgggtagagc ttccaagtaa tttaaatgct aaagaattaa tgccaaaatg tttagataag
     4741 aatgttgctt atgttgctgg aggaggattc ttccctaatg gtggtagaga aaatactttc
     4801 agacttaact attcaaatat gccagaagaa aaaataatag aaggtataaa aaatatagca
     4861 gctgtattaa aagaagctat gggtgtagaa gcttaattaa aaataataac ttatatataa
     4921 tacaagggat ggttgttgcc atcccttgtt tgtttgaaaa gtatataatt caccttatac
     4981 taaactttgt ccaaaattta aattgacaga aataagaata aatgataaaa tgtgtaggta
     5041 tgagatttta gtctcatttc tctgctctaa gagtgtagag ccgttaagtc caaagggagg
     5101 tgaattatag tgagaaatta tgaattagtt tatgtggtaa agccaaactc tgatgaagaa
     5161 gtaagagagg ctatactaaa caaagttaag gaagttgttg caactgacgg agaaatagtt
     5221 aaagttgata cttggggaac taaaaaatta gcttatccaa tagctaagtt tacagaaggt
     5281 ttctatgtat tagttaactt caaatcagca gtagacgttc ctaaagagat agacagaaac
     5341 ttaaagataa atgaaaacgt aataagacac atgatagttg ttgcttaata actggtttta
     5401 ttataaaaaa taggtggtgt ttttatgaat caagttgtat tagtgggaag gttgactaga
     5461 gaccctgaat taagatacat cccaggtaca ggaacaccag tggcatcctt tacaatagcc
     5521 attgatagag actatgtaaa aaaagatgga tctaaagaaa ctgattttat acctgttgaa
     5581 gtaatgggta aatctgctga attttgtgct aactacataa ctaaaggtag attagtagca
     5641 cttcaaggat ctataagagt agacaattat cagacacaat caggtgaaaa gagaactttt
     5701 acaaaagtaa gtactagatc agtacaagct ttggatagta agaataaatc ggaaaattca
     5761 tatagagaga gtgctccagc ttttgagcca agctttgaac ctcaaggttt agacccacaa
     5821 ggtttccaag ctatagatga tgacgatata ccattttaaa aaggagggaa aaaagtcatg
     5881 atgaataaga aaagacgtaa gaaaaaaaga gtttgtcaat tttgtgctga caaaaacgct
     5941 aaaatagatt acaaaagtac tcaaagatta caaaagtata taactgaaag aggaaagata
     6001 ttaccaagaa gaatatctgg aacttgtgct aagcatcaaa gagaattaac agttgctata
     6061 aaaagagcga gaaatatagc acttttacca tatacattag actaaaaaaa tgagccttgt
     6121 gctcattttt tattatatgt atttatatat ctaaatgagg cgagctataa agctcgtttt
     6181 tttgtttata tacttaaata tattattatt tatgatataa tattatatat taaggagggt
     6241 tgtaaacgtg aaaatagata aaggttctga gataacgaga aacataaaga taatagaatg
     6301 gatgaaaaca gaaatattaa tgagtgtaag tgatttgttt aatttattgt ttaaaggggt
     6361 aaaaccatta gatgaagcaa ttcaagatac tttagcaaat ataataatga taacatatct
     6421 cttagcaaag agattaggaa ttagttttag agatgtggat tataaagtca aggaaaaaat
     6481 aaaaattggt atagatgagg accatagcgt tgaaagatgg tatggagatt tatctaattt
     6541 aaaaaaacat atggataata gggagtgata ttaaattgaa taataaaatt agactatcta
     6601 aggcaatgtc tataatagtt ttagctataa taatcgcttt agtaatagcc tatattccaa
     6661 tgctaggaat atttagtatg ttagcagctg ttccatatgt aattataggt gctattactg
     6721 atagaaagta ttctcttata tctattttgg ttacattttg tgtactgatt ctatttgcag
     6781 acatttctta tgcattaaat atatgtataa tgtattcttt accaggtata gtaataggaa
     6841 agatgctaaa gagaagcttt gaacaagagg acagtaataa attcgaaccg atatatggtg
     6901 gaaccataat atttgtactt tcaatgcttg tatatttttt tgtattaaag acatttttaa
     6961 atgtgaatct attagatgaa gtttctaaga tgatatcaga aattgtaaat attcaaaaaa
     7021 atagtttttc tgcaactgaa cttaaagtgt ttgataaaat gaagccagat gagattgtaa
     7081 gttattttac aaatatgcta ccaatgatgt tatttttaca agggttgtta tcagcgtttg
     7141 tcacatatta tttgagtgta ttttttataa aaaggattac aaagatgaat ataagatttc
     7201 ctaagtttgc tgatttttat ttaccgggaa atgctatact cacaactttc ttactttatt
     7261 tattagtctt atttattcaa attattggtt caaaattata tacagaatta ataatgacta
     7321 atttacagct agtttttaat ttaatgtttg taattcaagg tatagcagtt tgcatttatt
     7381 tcttaaagaa atggataaga cagggaccaa ataaaattat gtttttttca gggataattt
     7441 tatgtttatt tggatttatg ggaatatctt ttgttggtat ggtagatagt ataattgact
     7501 ttagaaaggt aagaagctat aaatccactt aggaggatag aaatgagtaa taaacaaacc
     7561 tttaaattaa atatgccaga aataaattta tatataattg ttattggtat ttctagtata
     7621 attttgcttt attacaactt gtatgtaggg tgtttatttt tctgtatttt tgtatatatg
     7681 gtttttcaca attggagaac tactaatatt agacgtcatg aatggacaga gtatattcaa
     7741 aatttatctt tagatataga tgaaacaact aaaaaagcca taataaatct tccaatacca
     7801 ttatgtattt tagaatttga tggaaatata tcctggtata atggaaagtt ttatgatatg
     7861 attggtcaaa aagatttact tgataaaaat atagaggata tagttaaaaa tcttaattta
     7921 agaaaagtat taaatgaaaa taaagagatg tacacagaaa taaactataa agaaaaagaa
     7981 tatacaataa tttataatgt tataaaaaat gatcaagaaa aaaatcctaa atatttgatg
     8041 atattatact ggatagacaa gactgaatat ttaaaagtaa aacaaaatta tgatgatgaa
     8101 aaaaatgcaa tgatgctaat acaagtagat ggatatgatg aagtattaaa aagtgcagca
     8161 gaagataaga gagctttaat taatgttgaa gtagagaaaa tactttctgc tttagaatta
     8221 aattcaaatg gtgcattgag aagaacttca aaagacaaat tcttcttggt aatgcataaa
     8281 aaagagctaa aaaaattgga agctgagaag ttttctattt tagatacaat aagacatatt
     8341 gattatggaa ataatcttcc tgttactata agtataggaa ttggtataga tggagataca
     8401 ttaaatgaga acctaaaact tgctacgggt gctctcgatt tagcacttgg tagaggtggt
     8461 gaccaggctg ttgtaaaaac aaaagataaa tttgtgtttt atggaggaaa atctaaagct
     8521 gtagaaaaga aaaccaaagt aaaatccaga ttaataggtc atgctttaag agaggttata
     8581 caacaaagtg accaggttta tataatggga cataaatatc ctgatatgga tgctatggga
     8641 gcagcagttg gtgtttatga tatatgtaaa tcctgtaaca aaactgcaca catagtttta
     8701 caatctgtaa atgaatctat agaaatattt ataaataaaa taaatgaaaa taattactat
     8761 aaaaagttgt ttattggaaa agaggaagct attgacaact gcactaaaaa tacattagtt
     8821 gtagtagttg atactcatag accaaattat actgaatgtg aagaactgtt aaagctatca
     8881 gagaaggtag tagttataga ccatcataga agaggtgtag agtttataaa tgatgcagtg
     8941 cttttattcc atgagatata tgtatcctca acgtgtgaaa tggttacaga gttagttcag
     9001 tatatggatg aagatgtaac aataaataaa cttacagctg aagggctttt agcaggtata
     9061 agtctagata ctaaaaactt tgcattcaaa acaggtgtta gaacttttga agctgcttct
     9121 taccttagaa aagttggagc agatactata gaggtaaaga aattcttcaa ttctgatgta
     9181 aaagatttca taataaaagc tgaaataatt caaagtacta aaataattaa taatagaata
     9241 tgtttagcat attcaagtac tgagatagat agtataaatg ttataatagc tcaaactgct
     9301 gatgagttat taaatatcaa agaagtagaa gcttcctttg tattaggtga aaaagatgat
     9361 actatattta taagtgcgag gtctttagga caaataaatg tacatgtact tatggaaaaa
     9421 ttaggaggcg ggggacacat agatatagct ggagcacagt taaaaaatgt ttctctaaaa
     9481 gaagcttata agatggtaaa taaaataata gaggagtatt tggaggagga agaatagatg
     9541 aaagttatat tattaaaaga cgtaaaagga actggtaaaa agggagaaat gaaagaagta
     9601 agtgatggat atgcaagaaa tttcttattt cctaaaaaga tggctgtgca agcagatagt
     9661 gtagcaataa aagaattaaa tgaaaagaat aaatctaaag aaataaaagc acaaaaagag
     9721 tatgaagaag cagttttatt aggtaagcaa atggaagaaa ttaatataga aatatattca
     9781 aaagcaggag aaggtggaag attatttggt tcaataactt ctaaagaaat tgctgagcaa
     9841 ttaaagaaac aaaaagacat agatgtagat aaaagaaaaa tattattaga tgaaccaata
     9901 agatctttag gttcaacttt tgtagaaata aaaatacatc aaaaagtaac tactaaaata
     9961 agagtggatg taaaagaaaa acagtaataa ctagaggtga ttaagatgga agatatgacg
    10021 agaattcctc ctcatagtgt agaatcagag caatcaatat tgggttctat acttctagat
    10081 aaagatgcta taataacggt tacagaaact ataaagcctg atgatttcta taaagaggct
    10141 cacaaaataa tatatgaatg tatgataact ttaagtaata aaggtgagcc tatagacttg
    10201 ataacattaa cagaagaact aagaaaacaa gggcatctta atgatattgg aggaatcagt
    10261 tatattacaa gtctttctac tatagtaccg acgacttcaa atgtaaaata ctatgctgat
    10321 atagtaaaag aaaagtctgt acttagaaag cttataaaag cttctaatga aataataaat
    10381 ctaggatata gtggagcaac taagattgaa gatgttttag aacaagctga aaagagtata
    10441 tttgatatat cacaggagaa aactagtgat gattttaaat ctataaactt agttttgatg
    10501 gatgcctatg atatgataga aaaattatac actaacaaga gtgatgtcac tggtataact
    10561 actggtttta aagatttaaa caaaaaaata aatggtcttc aaaggactga tttaatatta
    10621 attgcagcta gaccagcaat gggtaaaact gctttttctt taaacttggt tcaaaatgca
    10681 gcattaaaag gtgatgcctc tgtagctgta tttagtttag agatgtctaa ggaacaatta
    10741 gtacaacgta tgttatcttc acaatctagt gtagaattaa aaaaaataaa gactggaaca
    10801 cttaatgata atgattggcc tagaattata gatgctatgg cagtattatc agatgccaaa
    10861 atacacatag atgatactcc tggtataaaa atatcagaac taaggtcaaa gtgtagaaag
    10921 ttaaaaatag aaaaaggact agatttagtg cttattgatt atcttcaact tatggaaggt
    10981 gaaggtaata atgaaagcag acaacaagag atatctaaga tatcaaggtc actaaaaata
    11041 ttagcaaaag aattaaactg tcctgtagta gcactatctc agttatcacg tgcaccagaa
    11101 caaagagcag accatagacc tatgttatct gatttaagag agtctggagc aatagaacag
    11161 gatgcagata tagttatgtt cttatacagg gacgaatatt atcatgctga ttctgagagt
    11221 aaaaatatag gagaagtaat aatagcaaaa aatagacacg gtgaaactgg ttctgtggaa
    11281 cttgtttggc ttggtgaagt tcaaagattt ggggataagt tgagagacct ataatctaac
    11341 gaatatccac agaacgtcct ttctttctac aggaaaaatg tggatacatg tcactaaata
    11401 tgtccaaaca ttgaaattta gctaaaaaac tatccacact atccacagat gacctgttga
    11461 taagtgtgaa taagtagata aagcaagctt ttggcttgct ttatctgtgt ataaatatta
    11521 attattagga ggaataatat ggaaaaaata agcttaaaat atatatatcc caatataatt
    11581 aaagtacttg atgaaataaa cttatttaga gtaattgaca ataatttaag agaatctatt
    11641 gttgtttatg ctaataatgt ggataatcag tatcatataa acatgactaa tacaaacttt
    11701 ggaaacataa ttaacatatg taaattagaa aaacttttag atgtggataa atttatggaa
    11761 aaagttataa aatatgaaaa agaaataata gagaaagaag agttttcaaa aatagaagaa
    11821 tatatgttaa atattggaga atactaaaaa agttgaaaaa aatacgaatg tttgatagaa
    11881 taagtaagta aagtgttcga gagtatataa aatatagagg tgaattacat gaaaacagtt
    11941 gcaattgttg gttctcaatg gggagatgaa ggtaaaggta aagttataga ttatcttgct
    12001 actcaagcag atgtagtagt aagagggcaa ggtggaaata atgcaggaca tacattagtt
    12061 gtagaaggta aaaaatacgc attacatcta attccatctg gagttttaaa tccaaataca
    12121 gtgaatataa tcggaaatgg aatagtgttt gaccctaaag gatttttaga agaattggaa
    12181 atgtttaaaa cggataatat aagtacagaa aatataaaaa taagtgatag agcacatgtc
    12241 atattcccat atcataaaga actagatgca ttgtcagaag aagccagagg cgatttaaag
    12301 ataggtacaa caaaaaaagg tattggacct tgttatatgg ataaaacaga gagatctggt
    12361 attagaatat gtgacttaat ggataaagat aaatttgcta taaaattaaa agctcaaata
    12421 gatgctaaaa atgaaatagt taaaaatata tatggaaaag aagaattatt tgattttgaa
    12481 actatatata acgaatatct aggatatgca gaacaaataa gaaaatatgt ggcagatacg
    12541 tctgttattg tatatgatgc agtaagagca ggtaaaaaag tattgtttga aggagcacaa
    12601 gggactttat tagatttaga tttaggaact tatccatttg ttacatcttc tcatccaaca
    12661 tctggtggat ttgcaattgg tgcaggtata ggaccaaata tgataaaaga cgttgttggt
    12721 atagtaaaag catatactac aagagttgga gaaggaccgt ttgttacaga gcaaataaat
    12781 gaaactggag ataagataag agaacaagga catgaatttg gggtaactac tggtagacca
    12841 agaagatgtg ggtggtttga tgcagttata gttaagtatg cagctagagt taatggattg
    12901 actagtattt catttatgtt acttgatgta ttaactggat ttgataagat aaaagtttgt
    12961 acgtcttata aaatgggcga taagataata acggatttcc cagcttcatt agatgattta
    13021 gcaaaatgtg aacctgtata tgaagaatta gatggatgga atgaagatat aactcaaata
    13081 gataactttg atgaccttcc agaaaatgca aagaaatatg tagcaaaaat agaagaacta
    13141 gttggagtaa gtgtagatat ggtttctgtt ggtccaaata gagctcaaac tataataaga
    13201 agaaatatat ttgcttaata ttttatcaat taaataattt aaaatagttg aagaagagta
    13261 tctcaagata atgtttatag ttattagaga tgctctttta tatttgactt acattgattt
    13321 tttattaagt taaagctact tatacatatt ctagctgaaa ttaaagttga atttatgtag
    13381 atacatatag gttttttaaa aagaattttg ttgaaaataa atggctaaag atgattttaa
    13441 ttatattcta aaaaaagtaa aaaaaactat tgactaaaaa ttatatagac gctataatat
    13501 aacttgtgag tgaaagaaac gacgaaaaaa gtcgaaaaaa tgtggtctat tagctcagtc
    13561 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
    13621 aatatggctc gttggtcaag aggttaagac accgcccttt cacggcggta acaggggttc
    13681 aattccccta cgagtcacca gtatgggact atagctcagc tgggagagca cctgccttac
    13741 aagcaggggg tcacaggttc gagccctgtt agtcccacca aattttgaat tagagataaa
    13801 tactagatta tatgctggtg tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt
    13861 gtaggttcga ttcctatcac cagctccaca aagtacataa attcaaatta atataatttt
    13921 ctatgttaca ggatactttt aagtatcttt ttttaatttc atttttattg tattaaaaat
    13981 gaaattaaac atacaacaaa attttgaatt acttgcaatg tgatagtttt aaatataaaa
    14041 tttagaatca ttctagcata gtattatgta atatagcaaa tttaatttat agtatcaata
    14101 aatatagact actaagaatg taaatgattt gttagattaa gatacattag atatgtaaat
    14161 agaaaaataa aaagtatgtt ttaatttatg tgatataagt tttaaacaga gaagcaagtt
    14221 tgctcttacc tctctattta aaaataaaaa aatgtttttt aagggctatt ttatttaaat
    14281 ctttcccatc tcaaatcatc tccagtaaat tctaaaatcc taaacttatc taatattcta
    14341 gagagagttc tttgagtgta agtatttcct atttgagatg gtgttaagtt ggttgaaata
    14401 atgatttttt taccagcaac aagtcttgta tttacaatat tgaagatttc tccactagta
    14461 aaagaattat taagttcagt tccaaggtca tctattatta ataaatcaca atcaaacaag
    14521 tttttataat tatcttcact tatttgatta tttgtgtctc ttctaaattt ataatcttca
    14581 agaatatcta aaattctaaa agaagtttga tagattacaa cattgccttt atctaacaat
    14641 tcctttgcaa tacaattgca catataggtc tttcctagtc cagtagaacc ataaaacagt
    14701 agattttcac tattatcctt cttaaaatca tgaacaaaat tttcgcagat acttaaatta
    14761 tttagcatat tttctctagg agatatttca ccatcagagc ctttcttagg agaaaatata
    14821 ttcaagttaa aattcgaaaa attttcctga cttaaaatcc tatctaaatt agacatttta
    14881 taagcttcat ttacaatttc ttgtttgagg caagaacatt tttctccatt aggtaaaaat
    14941 cctttatctt tacaagaaat acattgaaac tttatttcca agtaatcaag aggtatgtta
    15001 ctttcagcta aaagtctttc tttctttact tttaaagact ctatattctc tttagtcttc
    15061 tttacaattt catctttaga cttgggattt aaaagtacta tcttagctaa actgagacct
    15121 attttagaaa tttcatcatc tatagatttt atctcaggga ttctattata tacctcattt
    15181 tttctatgtt ctaataagag ttcattatca tctcttctct tagcatattt agcaagtatt
    15241 tttcttattt tatcctcatt catttaatca cctacttaaa cttttctttt tgactttttt
    15301 taataatctc atcgagctca tcagaggtat actgagtgaa tgtttcgttg aaattatgaa
    15361 acttagtttt tttgtaagta ttgttgtttt gagtagtatt aatctgcttc ttattaatat
    15421 tctccttaac tatacgttct tcctcttttt gctttaaatc atttaaattt tttatatttt
    15481 tttcattcca atttttaatt ataccattta tataagatat acttgggttt gaaatattct
    15541 tagacttaga acatgcattt attatcaaat ctatatccat attaaattta tcaaaccagg
    15601 tgtccataag ttctttttct gattttgatg gttgtcttga aaatcccaat tcattgaaaa
    15661 tcgttttata taaaatatat cgttcacttc ttaccaaaaa gctttcttca acatcttttg
    15721 gcgtatataa atttgaatca taccaatttc taataatgcc ttcaatgtat ttaacgggct
    15781 ttgatgtacc agttttatct ttgacgtatt catatgcgca aagtatcata tcggggctca
    15841 tattgtattt attcattata tccataatac tcatcttttc gcttgggtcg agatatcgcc
    15901 caactatttt atttatagaa ttaaacattt ttctaatact tggattttca gaagtagata
    15961 caaccttatc tgtattagat tttatagatg aattattgct taatatattc tcaatataga
    16021 aattttttaa atctaaaaat tctattgaat aattaaaatt atcatattct ccattgtcat
    16081 gtatttttat aatcttcttt tcttcccaga atttccaagc agataacaca tcagacaatg
    16141 gtatatttag gtttttggct attgaattat tgtcgaactt aggattagaa gtaatatcac
    16201 atgcttgtct atatcctaaa agataaactt ttacgtataa cccgtccgcc attggcataa
    16261 atatatctat aaagatattg ggaatagtga tttcacctaa atctatttca ttgctttctt
    16321 taaaaaacat tttatcacct cagattaaat tatatcattt tttatattat atttgtctgt
    16381 aaacaaataa aaggtcacaa gattagcatg taaatatttt ctaaaagtat tcattttgaa
    16441 aaggaggaga tattttggtt ataaaattca accaaaaagt aagaagaaca ttatcagtag
    16501 cgattctatt tatggtatta ataataggtt gctttataga aaaggatgac agtttagaaa
    16561 catacaaaga aaaaactaat tcaaaaataa atcagtataa tatggatgtt atatttgatg
    16621 atgagactaa gaggttaatg tgcaatcaga atgttgacta cataaataat acaaaatcaa
    16681 acatagataa aatatatttc catatatatc cgaatgcttt ttccaagaaa gattttgctc
    16741 cttttgaaaa aagtgaaatg acaagggctt atcctaatgg atttaacgaa ggatatatag
    16801 atataaagaa tatattaaat aataatagta aaatggagta taaaattaaa ggagacaaaa
    16861 atgacatatt agaaatagaa ttagggaaag agttaaaacc aaatgaaaga atgtcattag
    16921 atataaaata taacgtaaaa ataccaaatt caattggaag atttggatat ggagaaaata
    16981 ctataaatgt tactaattgg tttcctatag cttgtgtaaa tgatgaaagg ggatggaatt
    17041 taaagagtta tgaaactatt ggcgacccat tttatagtga gactagcgat tttcatgtga
    17101 aactattgat tccaaataaa tataaaattg ctcatacagg aaaacttatt gatgataaac
    17161 atgataataa gaaaatgcta tatgaaatag aagctgaaaa cgttagagat tttgcattta
    17221 tactaagtag caaatttgat gtaaacaaag tagattcaaa gggagtagct atcaatacat
    17281 ataatttaaa taaaaagctt tctaaaaaag caactgaagt ggcaaaagat tcaataaata
    17341 tatttagtga attatttgga aagtatcctt acaaagaatt ttctgttgta gcaagtgact
    17401 tttttatagg ggggatggaa taccctatgc ttgttatgat agaccaaagt ttatataatg
    17461 aaaaaaatga atttttacta gaatatgtta ttgcccatga gacagcacat caatggtggt
    17521 attctgctgt tggaaatgat gaaataagtg aaccctggtt agatgaagca cttactgaat
    17581 attctacaat agtttatttt gaacaaaagt atggtaaaga tatggcaaac aagcttataa
    17641 aaactatgga gatacagaca aaaagttatt tgagcgaaaa tatatttaag ccagcaaatg
    17701 agtataaaaa ttctactgaa tacagcttga atgtttatac aaaaggcgca atagcattta
    17761 atgaaataag aaaagaagtt ggagataaag tattctttga gactttaaat gagtactata
    17821 ataagtataa gcataaaaat gctaatggaa gagcttttgt ggaactatgg aatagtaaag
    17881 gtgtagatat aaataagatt ataagtgaat gtaagtaaat agtgaatgta agtaaatttt
    17941 gtgcagagta ttaaatttta tttaatactc tgtttttttg gtataatata atttataagg
    18001 cagaaaaaga ggtgaaggaa tgctaaagta ttgttcagta gggagtggta gtagtggtaa
    18061 ttgccactat atagggtata aaaatactaa tatactagta gatgcaggat taagtggcaa
    18121 gagaataact acaggtctta aagacattgg tgtagatgca gataaattaa aaggaatatt
    18181 tataacacat gagcatgttg accatataaa aggagcaggt atattatcaa gaaagtttga
    18241 tataccagta tttgctaata taaaaacatg gtgttctatg aaggacaaat taggtgacat
    18301 caaagataaa aatatgaaag tatttgaaaa tgataaaacc tactcattag gtgatatcat
    18361 agtaagacct ttttcaatag aacatgatgc gtctgaccca gttggatata atttttatac
    18421 agagagtgat gaaaaaatgt ctatagctac tgatatagga tgtataactc aaaatataaa
    18481 aaaacatcta tataaatcta aattagtagt attggaatca aattatgacc ctaatatgct
    18541 tatgatgggt tcatatacat atgctttaaa gaaaagagta atgtcaagta ctggtcattt
    18601 atccaatgaa gatgcagcta atttctgcgt tgaacttatt aatgaaggta cagaatctat
    18661 attattagca catttaagta aagaaaataa ttttcctgag ttagcgtatg agacttcaaa
    18721 aggagtatta gcatcaaatg atatagtagt tggacaagat gtaaaactag atgtactatc
    18781 acgaaatgat gtttctaatg tttatgagat gaagtagagg tgaataactt tgctaaagag
    18841 aaattttgaa aatgttgagg ataataaaat tttatactct ataaagcagg gtatgatttt
    18901 agcgatacct gctattatga ctggttcaac tgctcttgta attttgaatc ttccaataaa
    18961 ggcttatcaa gaatatttat caggcttgtt taatggagag gttacaaata ttcttaactt
    19021 tataaatgat tctactttag gaattatttc attaataata ttattaacaa taagctatag
    19081 ttatggaaaa atatatggga gcaagtatac agtacttgtt cctatagttg caatgtgctc
    19141 ttttttagta ttttcacatg gtaatgagct taattcttat atagaaatat ttaaaacaaa
    19201 gtggttattt acatctataa ttgtttctat gacatcttct gtattgtttg taaaacttac
    19261 agaatctttt gttacaagtg taaagtttca tacagaaggg gctgatgcag actttaatat
    19321 ggtagtttcg gctatagttc ctttcataat agtagtattt ttgttttcta tttttagagt
    19381 gataatgata agtctaattg gcagttcaaa ctttcaagat atattttata atttattttc
    19441 aaatgttttt aataaaatgg gaacaaacct tatgagtgct ctgttgttta tttttttaat
    19501 gcactttatg tggttttttg ggattcatgg tagtaatgta ttagataccg tcgcaaaaaa
    19561 tttatttgaa aatagtatgg caattaacat aaatttagtt aataataatc aattaccaac
    19621 agaaattttt actaagacct tttttgatac aatggtttta ttgggaggat gtggttcttt
    19681 actatgttta gtaattgcaa tttttcttag tgaaaaacgt ataaatgtaa gaaagttagc
    19741 taaaattgct tcaataccag ctctttttaa tatcaatgag atgctagttt tcgggatacc
    19801 aattgttttt aactatatta tgtttgtacc ttttgtaatt actcctataa tcttaaccat
    19861 tacaagttat gtggctatgt ctacaggtat tgtaccatgt acagtttcag ctgtagaatg
    19921 gacctcacct atatttttaa gtggatatat gtctacaggt tcaataagag gtagtttgtt
    19981 gcaagtattt aatttatgtg ttggtgttat gatttatatt ccgtttataa aaatgtcgca
    20041 aaatagatat acatatatgt ttaaaaataa tatagaaaat ttaacaaact tattaaaaag
    20101 gtatgaatta acaggtgaac agcctaatct tctaaaccat aatggtaaaa taggttcaat
    20161 ttcaaaaatg ttggcatcag acttgaagtt tgctatgaaa agaggagaaa tagagctttt
    20221 ttatcaacca caagtcaatt ataatggaaa tgttgtggga gcagagggat tacttagatg
    20281 gaaacattca ataggagggt tcatttatcc accactagtt ataattttgg ctaaagaaga
    20341 agggttctta gatgagcttg gagagtatat tattaataaa gcctgtattg atttaaagaa
    20401 gagtgaaaag ctattgagaa atcctgttaa gttctctgta aatatatctc cagaccaact
    20461 tgatgaccct aagttaccag agcaaataaa aaatatcatt agtagtaatg atataaatcc
    20521 taatatgtta ggtatagaaa taacagagca aattgcatta tcaggttcac aaataattat
    20581 tgaacgtatc aatgcgatac ataacttggg aataaagtta attatggatg attttgggat
    20641 ggggcatagc tctcttatat atcttcaaaa taataacttt gatatagtta aattggatgg
    20701 ttcattagtt aaagagatac ttacaaataa aagaagtagt gaaattataa tgactattgt
    20761 aaatctttca aaaaatttag attttgatat agttgttgag tatgttgaaa atatagacca
    20821 aagggataag ttatatggtt taggatgtga aatatatcaa ggatattatt atagtaaagc
    20881 aatacctttt gaagagtttg tagaatatgc aaatcaagaa agagagatat aattttataa
    20941 gagcttgtaa attctagtaa tgcatagatt tgtaataaaa ttaagtttag gagaaatata
    21001 tgaatataag tatagtcgta gtaggtaaaa ttaaagaaaa atatttaaaa ttaggtattg
    21061 atgagttcaa gaaaaggctt tctaagtatt gtaagttaga aattatagag ttggatgatg
    21121 aaaaagctcc agagaatttg agtgttaagg aaatggaaat tataaaagac aaagaaggaa
    21181 agaaaatttt agggaagata aagcataata gttatgttat agccttggct attgatggga
    21241 agggtttatc ctctgaagaa cttgctaaga ctatgagtga tttagcagtt agaggtaata
    21301 gtagtctctg ttttataata ggaggttctc tagggctttc tgatgaggtt ttaggaaggg
    21361 ctgattatag gttatctttt tctaggatga cttttcctca tcagatgatg agattgatat
    21421 tgttagaaca gatttatagg gcttatagga taaataatgg agaaccgtat cataaataaa
    21481 tgatacggtt ttttatgata taattgaatt ataaaataaa atcaaaatgt aaagtaggtg
    21541 tagtaatgga cagactagat atatttatcg aaaattatga ttttaaagaa tatcttgaaa
    21601 aattacctag agaattaata gaagaaaatt ataaagattt tgttatagaa ttttggaaaa
    21661 atcctagtgt agatattatt aaactaaaat caaaatatag tataaaaaat gtagtatcta
    21721 taagtagtat attaatgcaa aatcctatcc aagagtatta tgagtttctt gaagatattt
    21781 ggaaaaataa cataaaaata gatgaattaa ttaataagta tttaattaca gcaacaagaa
    21841 tggatcaaat taaattccca ttaatatata ttgatgaaga ttctttttgt ccttattgtt
    21901 taggtaataa attcaatatt attaataata ttaagcaaca agatataaaa gttgaatgtg
    21961 aaaagtgtgg tgaggcaatt agttatttta ttcataagga agaagtagat aatataatag
    22021 aagaagaaaa aagaaaaaaa gaactattta taagaacatt ggagaatgta tataataaaa
    22081 taaacaagat taaatgtcat agatgccaat cagatttata tgtgtgtatt aatgataaaa
    22141 taatggagta taatattagg tgtgatagat gtaattcaca gtatgaatcg gttgaaattg
    22201 cacaggaaca atatagatta tggcaacaaa gatctgcaat gatggctaac ataaaaaata
    22261 aagaaaatga attgttgcta aagacactat caattaagga tgaaaaagat attaaattta
    22321 aactcgaaga tataattacg gagagtgaaa gtatagatgt aattagtgat ttagtagata
    22381 tgcaagatag aggagagaat attttcaagc aattatttaa taattatgtt agactaggaa
    22441 gattagacag aaggttattg gtaactgtgt gtaaagattg caagcaaaat gaaaatactt
    22501 atacatggga aagaggcaaa gaacatttaa taaatatgag ggggattaca tacagtgaag
    22561 cagttgtata tgagttaatg gaaaaaacaa agctattgcc aataagagca attattagaa
    22621 gacttatgga gaaaaaacta ttgtattgtg atgaaaggag caataattta caagtacacc
    22681 ctgttttgat tgaaaattta gctaaactag aatcaataaa taaatctcaa aatataaata
    22741 atcaagtagc ttatctaata ttcaataagc aaaaatatat atgctatcat tgtggtgaat
    22801 caggaagacc cttaaagata ggatatctaa caatagataa aaatataaat gatttatcaa
    22861 gtttagtggg aatatgtgat agttgctatg aagatattac tgaagatgaa attattatag
    22921 ataatatttt ttgtctggta gatgaaagaa acaatgaatt aggggaagct tggaaatttt
    22981 taatacaata tgatagtagt tttagaaata atacaattgc agaagtagag catgcaagat
    23041 tatatgatat gtatgatgaa gataatttaa ttcgtgcgta tgcaaataca ttgtataaac
    23101 aaacgaaaga tgggtttgct aatgaaaaag tattttttgc atatgcaaga gcaatattga
    23161 aaaatagcaa agggcaagta agtgtactac aaatagcaaa agaaaaattt aaattagaaa
    23221 agtggttaaa tggataaagt gataatacat attaaaatta taaatttata taaaactgta
    23281 ttgcaattaa gcaatatggt ttttatatat aagataatga tacaattaac caaaatgtgg
    23341 tagtacataa gtgttatgag atttaaatat tagcgattaa aataatgatt aagtaaaatt
    23401 gtaattgatg aggagtatta acgtattagt ttatttttat atataattaa aatataggag
    23461 ggaagaatgt gagtcatagt aaactatctg atcacaaatt aaaaaagggg aaatttatag
    23521 caccttttaa tgaatttatg acaccattga atagtaatga gtcatggtgc cttggtcgat
    23581 tgccagagta tatatggata ggattattaa taaataaata tggtagaaaa aaagggatgg
    23641 aaataattta taaaataatt gtaaaacttc attcaattga atcagatatt gtaacaccta
    23701 ggttgtctga gattttatct atggaggatg taaatcagga tgaattcttt agttatattc
    23761 taactattgt tgaaaaggaa gctttatctc cattgacatt aatatttaca tatagtaatt
    23821 atcctatttt tgttaaatat tttgcagagc caagtataaa aattgaagat agggttacct
    23881 atttagatga gactttaaga aaagctatgc atcatcaaag tgaatttgct acagacataa
    23941 gatttatagt tttatattat ttttgtatgt caggaaaact cgtattgcaa aaagaacaga
    24001 ttaataattt gctggaatat cctaatttag aacacactga tgaaaaaatg agaatgttac
    24061 gtccgtttat tcgtgcagca gaattaatgg ttttaaatat ggaaaaaaaa tctgattggt
    24121 atttagatat tttttggggg aggattagtg ttatgactga atgtaaatta tacgcaacta
    24181 aatttgaaga tgaaactgct aatacagact tgtatatgga acaattacat gaggtttttg
    24241 tttatttatc agatttacta tgtaaagtaa atccattaga cgagaaaatg ttagtattac
    24301 ttggaatagc aatatattct tataaacgat tacgagaagt agttgagcat aaattattta
    24361 attccatttc aggtagaggc tcagtaaggg tattgataga aaattatatt atgatgaagt
    24421 atttagtaaa aatagaagct caacatgaaa atatatggtc agaatatcaa ttttatggaa
    24481 taggtcaata taaattaatt gtggctagat atagagaaca agaacaagat ttaaaaaata
    24541 gtcatgttga ttataaatat attgaagctt tagttaatga atttgtaatt gaagaaacaa
    24601 tagatatgga tactaaatat tttgataagc aaaatatacg tagtaaggct gaaatagttg
    24661 gagaaaaaga gttatatggg ttgtactatg attatgattc cgcatatgaa catggtttat
    24721 ggggatcaat aagagaaagt tcaatgttaa agtgtgataa tccagctcat cactatcatt
    24781 gtataccaga tgtagacaat aatcaaaatt taaaaagtgt ttggcatgat tgtgtaaaca
    24841 taatggaaaa aactttactt ttcttagata gtgtatatgg tataccacaa catttgctat
    24901 tagaggtgat aaaaaatgga aaagaatttt ttactaaata aaataaatag tattcagaag
    24961 gaatatttag aattattagt aaagttaaaa gataatatag atactataga ttatatttat
    25021 ataatagatg aaataaatat attttggtat tcatatagag atgttataaa gctttactta
    25081 gggaatatta cacaaaaaga caatgcatat atttttacag gtgcaacata tctagatgtt
    25141 gatgatttag aacattatcc atttgttatg atgggagata tacatatagt agatgattcg
    25201 ctatgtaaat attcaaaaac agtactagta ttatcaaatc aggaatttgc taaagtatta
    25261 aaagaccaaa ttatttcttg tatagatgat aatatcaaaa tattgtctaa ttattctttt
    25321 gcgatgttaa tattaccaat gaatttacta tatggtaatg atatgaaaat aatacatgaa
    25381 tattcaaata atatatttct gaactttttt tcaagaaaag acttaaactt agatacttac
    25441 tttgaagaat ttaactcaat cgaagatata gctctagcat tgaatccagg tatagagagt
    25501 tatattgttt ttgacgaagg tgaagattta actaaaaatc tggaagaaag atttaaaagc
    25561 tttattgata aggggaaggt acccctacca gcacataaaa gtgatgcttt taaattttgg
    25621 ctgataattt atggatttat aactcaggca ttagatatta tattgtcatg taaaggattt
    25681 aacctaactc cttatttaag atatagagtt acattacaat atgttttatt actaggcgat
    25741 aatgtttttg atattgaaga tctcagcaat atgttgttaa aatgtaaagt agctcatgta
    25801 ctttatgcaa tatttgataa aagcgaaata aaaaatattt cctttgttca attttataag
    25861 aaattaagtg aatataaatt tagctataaa gtagaagatg atatcaaaga aaatataaat
    25921 ataaatcaaa ttgctgaaat tttaaataaa aatattacat tgtttttaaa ttatatataa
    25981 atttatttat ataattacat tccttagggt acataacaga aaaaaggctg tcgcactaaa
    26041 aaatagtgct acgccctttt ttatacaaaa aaataaagct caagttcaaa agagcctggg
    26101 attcctgtat aataattgta tgctaaaaca aaatactata caaattgatt atactactta
    26161 tggtaataat tatcaattta aattaccact aaatattgat tatatgattc caaaagatgc
    26221 tatatttatt gatggtacaa aaatagagtc atctgccaat agatatactt ttgtttggaa
    26281 aaaaactata aacaaaaata tggttagagt gatgaatgat atatctaatt tcatattaaa
    26341 atgtgagatt gattttggta ttaaaataat ttatagaaat aaaatcaaaa tatatcattt
    26401 aaaaaaactt ttaaagaagc ttaaaaaaat atcaaaagaa tcaaatataa catttgttca
    26461 tggaaaagga aagaaaaaat ctatagcgaa attaaataat tatatcgata gattgaaaaa
    26521 atatactaaa gaccttcata taatgggtga acgaaatagt tattctaaaa cagataatga
    26581 cgcaactttc atgagaatga agaaagacca tatgaaaaat gggcaattga aaccagcata
    26641 taatatacaa tttggcgtag attcagaata catagtttgg gtaagttcag agcataaacc
    26701 tactgatact acaacatcaa taccattttt aaaatctatt gaagagcata caaaatttaa
    26761 atatcctaaa atagtagctg attctgggta tgaaagtgag gaaaactata catttttaga
    26821 gtgtaataat caattatctt ttataaagcc tgcaaattat gaaattagta aaactagaaa
    26881 atataaaaat gatattgggc gtattgataa tatggattat gatgaaaaaa atgattatta
    26941 tatttgtaaa aatggcaaaa aactatttgc taacaaaata atcaatagaa aatcaaaaac
    27001 aggatataaa agtaaaataa cttgttatac ttgtaaagaa tgctgtgatt gtcaatataa
    27061 aaacggttgt attaagggaa ataatagtaa gattcctctt gaaaatagaa ctaaaaattt
    27121 acaagtatct aagttatttc atgaaaaaag aaaagaaaat ttaaagagaa ttataagcca
    27181 agaaggttgt caacttagaa tgaatagaat tatacaagca gaaggaactt tcgctcaagt
    27241 aaaacaaaat atgaaattta gaagattttt aagctgaggc aaagaaaatg ttatatcaga
    27301 atgtattatt gtagcgatag ctcataacat ttctagacta cacaacaaaa taataaaaaa
    27361 taaatgtgga atatatcttc atcatttaaa agaagtattc taaatttatt atttatagtt
    27421 agatattatc tgaaaaaatg gtatttttat acctttattt gttatgcata attttactta
    27481 gaacataagc tttatataaa acaattaaaa atatatctaa ttaagataag taaaaaaagc
    27541 tgtcacagaa caaatttaat ttgctaatgc gacagcccat ttttgttaat ttgacactta
    27601 ataatattaa aataacaaaa aagtattgaa agtatattgc atttaaggta gaattaaata
    27661 aaatattata acattataat gtttatatag ttgttaataa aaatttggag gaaaatatat
    27721 gataaaaata atgttggcct gttcagcagg aatgtctaca agtttattag ttactaaaat
    27781 ggaaagtgca gctaaagaaa atggtataga gtcgcaaatt tgggcaatac cagaatcaac
    27841 tatacaaaat gaaatagaaa aatgtgatgt attattacta ggaccacaag ttagatatgt
    27901 attacctaag gctcaagaaa ttgctaagcc atataatata ccagtagaag ttataaatat
    27961 gatgcactat ggaacagtta atggagaagc tgttttaaat agagcgatag aacttaacaa
    28021 cactaagtaa tataaattat aatgtatttt taatatataa attgggagaa tcaaaggatg
    28081 gatgaaaaga ttatagaaat atcttttaat ataataggtt atgcaggtga aggtaaaggg
    28141 ttagcttttg aagcaataaa agaggctaag aatggaaata tagaaaaagc aaaagaatta
    28201 ttaaaagagt ctagggaagt tataaataaa gcacatagat atcaaacaga attaatacaa
    28261 aacgaagcta gtggaaacag gactgaaata agtgtcatac ttatacatgc acaggaccac
    28321 ttaatgaatg gcatgaattt tcaacagtta gctgaggaaa tcgttgattt acatttgaaa
    28381 ttacaagata aataaaaatt aaagataata aattaagaga gttcttaaaa gatacattat
    28441 gtgtagcaca ctaattgtta gattattgat tcaatattta gtgagtaata cattaggatt
    28501 attttgagga ctctttttat atttagttta aagttaataa atatttatta ttaataatat
    28561 aagattatat gttataatat tataacatat aatttaagag gtgaatcatg ttaagaaatc
    28621 aaccattata tatacaagtt gtagatatat taaagcaaaa aatattaaat aacgaatata
    28681 aaattgatga aaatattcca acagaaaggg agttagagca agagtttgga gtcagtaaaa
    28741 ttactattag aaaagctatt gagatattag aatcagaagg gtatgtagag aaaaaaagtg
    28801 gaaaagggac aaaagtaata agtaatagtc tttttaataa gctatcaaaa gcagagtctt
    28861 tttcaagtat acttttatct aaaggtcata agttatctaa aaagactatt tcaattgaaa
    28921 aaataataaa tgatacaaat tcacaagtat ttagtatatt tggagaaaaa tgtagcaaga
    28981 ttactagaat gtattactta gatagtaagc catatatatt ttttactcat tatattcctt
    29041 ttgatgaaaa tctaactgtt gaaagtttta ataatgaaag ttctttatat atgtacttat
    29101 ataggagaaa tataaatata actagatttg aagatgaatt ttttatagaa gaatcagaac
    29161 tagaggtatc aaaggctcta aatctagcta aagacatatt attaggaaga aaaagaaaga
    29221 cctatgatga gaatgataat atagttgagc tatcatttgc aagatataat acagaagtac
    29281 aaaactatgt aattaaatac gaaatataat aagaatcctg agtttattga aattataata
    29341 tgctcaggat ttttgtttag ttgtaaaaac aaaaaatatt ataacattat aatgtttaca
    29401 agcaaacatt ataatgttat aatattttta ataaacaagt gatattatca attacataaa
    29461 aattagttta tgttatgaaa tcgttattgc attataaata aaggataata tcaataatac
    29521 agggggataa ctatggaaaa tttcgcaagt tttttagagc gcaagattat gcctgtagca
    29581 gcgaagataa gtaatcagag acatatgaga gctgtaagag caggtataat agcaacatta
    29641 ccactaacaa tagtggggtc atttttcaca atattattaa acatacctat tgatgcttat
    29701 atggatttta tagctccatt taaagcaata ttagatatac catttagata tactgtagga
    29761 tttttatctc tatatgctac ttttgggata gcatcatcac ttgctaaaag ctacgattta
    29821 gatacaacag gtgtcggtat gcttgcagtt atggcatttt tagtttctac aatagttcca
    29881 actcaagtag ttgaaccagt aggtggagtt atagaagctg gtagatggat ggatatggca
    29941 aaacttaatt cgccatcatt atttggagct atcgtaacat cattaattac agttgaaata
    30001 tatcgtttta tgaaggaaaa gaatattaca attaagatgc cagcgggagt tccagtagag
    30061 gtttcaaatt catttactgc attattaccg acagcagtaa ttttaatgtt attctgggta
    30121 ataagacata ttttaggatt tgatataagt gttgcactaa gcgctatgtt atcaccttta
    30181 aaaggattct tatcaggaaa tagtttaggt ggtgggttac tgacagtatt cctaataact
    30241 ttattctggg tacttggaat acatggtcca gctataatgg gaccagtaat aagacctata
    30301 tgggacatgt ctatagcaga aaatataact gcattcacag aaggagtatc agctcatgca
    30361 atgccaaaca tatttacaga acaattttta cagtggtatg tatggatagg gggagcaggg
    30421 gctactcttg cattagttgt aatgtttatg ttttctaagt cacaatatct aaaaagttta
    30481 ggtagattag gatttttacc agggttattc aacataaatg aacctataat atttggagca
    30541 ccaatagtta tgaatccaat actagggata ccatttataa ttggacctat aataatgaca
    30601 attctatcat atgttcttac tattacaggg gttataccta tgatggtagc aagattacca
    30661 tttgcaatgc cagcaccaat agcagcagta atgagtacta actggagtat actagcaggt
    30721 atcttagtat taatcaactt tgtgatatca tttgctgttt attatccatt cttcaaagtt
    30781 tttgaaaaac aacaattaca aagagaacaa gaagaaatga gtgaaaattt ataaaaatac
    30841 atagatatag aggttttgta ctatataaaa tatagaaaaa gcttataagt tattaagggt
    30901 gaaggtagga ggaatgacct ttgcctttaa ttttatatat tttaaataat gtaatttgag
    30961 gaggagaaag atggaaagaa gattaggaat atcagtatat ccagagcata gtaacatgga
    31021 agatgataag gcatatataa aaatggcttc aaaatacgga tttaaaagaa tatttatgtg
    31081 catgctttca attacaagac ctaaggaaga agttaaaaaa cattttaaag agattataac
    31141 ctatgcaaga gaattaggat ttgaagtaat ccttgatata gctccaaaca tatttgatat
    31201 attacaaata tcttatgatg atttaacatt ttttagtgag ttaaatgcta gtggaataag
    31261 gcttgactat ggatatgatg gaagtaaaga agctatgatt tctttcaatc catatgattt
    31321 aaaagtggaa ctaaatatga gtaatgatgt agcatacttg gataatataa ttacttacaa
    31381 gccaaatgta aataatatat atggttgtca caatttttat cctcaaaggg gtacaggctt
    31441 gccatatgat ttttttgtaa gatgttcaaa tagatttaaa aagtatggaa taagaactgc
    31501 tgcttttgtt aattcaaaat acgcaacaat agggccttgg aatataaatg atgggctgtg
    31561 tacgcttgaa atgcatagaa acttagatat tgctacagca accaagcact tattcgcaac
    31621 aggattgatt gatgatgtaa ttgtagggaa tgcatatgca agtgaagatg aattaaaaag
    31681 aatgtctgaa gtaaataaat atcaagttgt atttaatgta gtaactgaag aaaaaacttc
    31741 tgaagtagaa aagacaataa tggcaaatga gcaacacttt agaagagggg atataaccaa
    31801 ccaagtagca aggtctacag aagtaagaaa aaaatatgct aaagaaaatt tccctaagca
    31861 tgataatacg accatcttta aaagaggaga tgtagtgatt ggtaatgatg actttggaaa
    31921 gtataaaggg gaattacagt tgatacttca agattgtgca gatgaaagaa aaaataaggt
    31981 gggtagaata aaggaagacg aaataatact tttagacttt attgaacctt ggagtaaatt
    32041 taagattatt ttagagggag agtaaagtga agatagacat attaataaaa tgtggaaaaa
    32101 cagtagataa aagtataatt gatatagcta tattagacga taaaataata gaagtaaaaa
    32161 atcagataga tgattctaag tatcaggcaa aacaaattat aaacttagat ggagagaaat
    32221 ttattagtgc tggttggata gatatacata cgcactgcta cgaaagacta gacctttata
    32281 aggattatcc agatgaggtt ggaataaaaa gcggtgttac tactgtagta gatgcaggaa
    32341 caacaggagc tttagatgta ggtaaatttt atgaagatac taaaagatat aagacaaatg
    32401 tatatgcttt aataaatatt gctaagcagg gaataacatc tcaagatgaa ctttcaagta
    32461 tgatgaatat tgatgaatat gaacttaaaa gagcagttaa aaaatacaaa gattttgttg
    32521 ttggaatcaa agctagaatg agtaaaagtg ttgtaatttc aaatgatgtt gaacctttaa
    32581 aagtagctaa aagaataaaa aatgaactta atctacctat gatggttcac tttgggagtt
    32641 ctcctccaac gatagaggat atatttgact atatggaaaa aggagatata ttaacccata
    32701 tatataatgg aaagccaaat ggaatactta gaggcaatga ggttaaaaaa gaaatcatag
    32761 aagctagaga aagaggtata atccttgatg taggtcatgg aaccgaaagt tttagtatgg
    32821 atattgcaat gaaatctaag gatgcaggaa tttttccaga cactataagt acagatatat
    32881 atattaaaaa tagaataaat ggtccagtgt ataatctatc tacaacaatg gaaaagttta
    32941 tatatatggg ctattcactt gaagatataa tagataaagt tactaaaaat gcagcagatg
    33001 caataagttt aaaaaataaa ggtttaataa aagaaggata tgatgctgat ttaactatat
    33061 ttgatgtggt taatgaagaa aaagaacttc aagattcttt gaataagtca gtaatcacaa
    33121 gtacgtctat aaagccaaga gcagtagtag tcaatggaga atatttaaat ataggtaagt
    33181 ctatagaaga gaatgagtag aagggagaaa ctattgtatg agtagtatat ttgaaaaata
    33241 ctctgtaaga cgtgttataa atgcttcagg caaaatgact atactaggag tatcaacagt
    33301 agataaggaa gttattgaag caataggtga agggtgtaaa aatttcttta taatagaaga
    33361 acttataaat aaaacaggtg aatatatagc aaatttatta gggtgtgaaa gtgcactaat
    33421 agtttctagt gcatctgctg ggattgcaca gagtgtggga gcaatgatag caaaagatga
    33481 tatgagtcta gtttacaatg taaataatcc taagaaacaa gttaaaaggg aaataataat
    33541 tccaaaggga cataatgttg attatggagt tccagtagaa gtaatgataa gacttggagg
    33601 aggagaagtt gttgaggccg gttatgctaa tatgtgtaac aaggaacata taataagaga
    33661 gataaatgaa aatacagcag caattatgta tattaaatct catcattgtg ttcaaaagag
    33721 tatgctaagt gtattagatg cagtacaagt tgctaaagaa tataatttac ctttgatagt
    33781 agatgcagca gcagaagaag atttaaatgt atattatgaa ttgggagcag acctagtgat
    33841 atacagtggt gctaaggcta tagagggtcc tagctcaggg ttagtaatag gtaagaaaaa
    33901 atacatagat aatgtaaagc ttcaatcaaa gggtatagga agagctatga aaataggaaa
    33961 agaaaatatt gtaggtctaa catgtgcaat tgagagatat ataagtaatg ataaagtaac
    34021 tttaaaagaa atggaggaca agctaaatcc atttatagac aagataaata ctataaaggg
    34081 agtgagtgct agtataacta gggattcagc aggtagagaa atacttcgtg gagaaattga
    34141 ctttaatgaa gatataataa ataagtcaac acaacaaatt ataaatgaac ttagaagtgg
    34201 agagatagct atatacacta gagactataa ggctaatgaa ggcaagattg agattgatat
    34261 aagaagtgta actggaagtg atttagatac tatttataat aggataaaaa atatagtaga
    34321 aagttagagt gtagagtaag tgacattata atgatttaat aaattaaatt ttaccaatgg
    34381 aggggataaa tatgaattgt ataccacaat attataaaaa tagagtttgt ttaaatgttc
    34441 ttgcaggaag cataaaaaat gctaaagaag tatatgatgc ttgtgacggg catgtattga
    34501 taggagtact atcaaagaat tacagcactg tagaagcagc tattgaagat atgaaaaaat
    34561 actcaaagga aatagataat gcaatatctg tggggcttgg agctggagac ccaaatcagt
    34621 caaatatggt atctcagatt tctaaagaac ttcagccaca acatataaat caagtgttta
    34681 caggtgtggc tacgtctaga gcattgcttg gtcaaaatga tagtataata aatggattag
    34741 tttctccaac tggtaaacca ggatttgtaa aaatatccac aggtccatta agttcaaaag
    34801 aaaaagatgg tatagttcct atagaaacag caatagctat gatgaaagat atgggaggaa
    34861 gctcaattaa gttttttcca atgggaggac ttaaaataaa ggatgaatat atagaagttg
    34921 caaaagcttg tgcaaagcat aatttttatt tagaaccaac aggaggtata gacttagata
    34981 acttcaaaga gatagttcaa attgcacttg atgctggtgt agaaaaagtt atacctcatg
    35041 tttatacatc aataattgat aaagaaacag gtgaaacgaa agtggaagat ataggagtac
    35101 tttataaaat aatgaaagaa ttattgggat agatttatga atatattagc ttttggtgaa
    35161 ataatgatga gactttcagt acctgactac aagttcttaa cacaaaccaa tgaactaaat
    35221 tatataatta caggcacggg attaaatata ttatctggac ttaaaaactt tggatacaat
    35281 acctatatgt tgacaaagtt gcctaataat aatgtaggta aagcatcaag tgcaaatata
    35341 agaaagcttg gagtaaaaga tgactttatc acgtatggag ggaatcatat aggagtgtat
    35401 tttcttgaaa atgggtatgg agaaagacct tctgaagtga cttatttgaa taggttaaat
    35461 agttcatttt gtgagagtaa attacaagat tatgatattg ataaatgttt agaaggaatg
    35521 gatatagtcc atatttgtgg tattgctatg caacttacag attcagttaa agagattgct
    35581 ttagaactag ctagaaaatc ttatgaaaag gggataaaag tattctttga ttttaatttt
    35641 agacaatcat taaatccaga aaggacttat gatgatttag taaatgatta taagaaaata
    35701 ctaccatatt gtcatgggct gtttgctagt tatagagata taaataagat acttaatata
    35761 gatagtctaa atataaaatc tagttcagaa ttgattaaaa aagagtatga tatagatatt
    35821 gtagctggaa ctatgagaag atttgatgat atgaataata gatttataaa gggatatata
    35881 tcaaatgatg atggttattt tgaaagtagg gagtataagt tagaaatata tgacagggtt
    35941 ggtgccggag atggatatgt agcaggaatt atatattcat acttggaaaa tatgaagagt
    36001 gaagatatag ttgagtttgg ggttgctagt tcggtgttag ctcatacaac ttatggggat
    36061 aattctttag taagcacagg tcatgtactt agactgatga acaatgaaaa aatagatata
    36121 ttgagataat acatttgtat ataataaaat ttttatcttt tttagacaca tttttgactt
    36181 gcttatattt gttaaacaga gttattttta acgattataa atattaaatt aaaagttaaa
    36241 taataaaata gtagaaaata gcattaacta tagaaaaaat ggttaatgct atttttatcg
    36301 ttaatattga ttaattttat attatagaaa tgaacttttc taatgctgga tttgtatttt
    36361 ttttattcca tacaactaca acttcaattc tattttctac ttcttttata ggtactattg
    36421 caatatcacg ttctatcatt gtatctgtaa cacaaaatga aggtaaaatg gatattccca
    36481 tgttagaaga taccaatgat tcaatagtat taagattatt tgaacgttgt accacattag
    36541 gtacaaaccc tgcttcacga tatctattct gtatcattga cttttgtcca tactcatccc
    36601 ctgtttcttt tacaaaaata aatttatcat gttgtaattc aattaaatcg atagaatcct
    36661 cattgtaaag tggatgacca ggaggtaaaa gagctaccaa agaatatctt tctacagttt
    36721 tatattcaaa atcttttaat ggttgatttt ctggattaat aacaaaagcg aggtccatag
    36781 aattattttt tactaagttt aaaagttcgg ttatttcatt agtacaaata tccatagata
    36841 tattagggta tgtattagaa aattctttta agtattgttg aagtttgttt ttttcatatc
    36901 ctattaaggt tccaatgctc aaagttccaa aaaaaccatg tgcaaattgt tgtgtttttg
    36961 caatagcatc attgatattt tttattatag tacgtgcctc agataaaaaa acttttccag
    37021 ctggtgttag ttcaacatga cgtttacttc ttataaatag agttacattt atttgttctt
    37081 ctaaagcttt tatttgctgt gtaacagcag tttgagaaat ataaaatttt tctgctgttt
    37141 tggtaaaact tagattttct gctacagata taaaatattc taattgttta gtattcaaat
    37201 aatcacccct atttaaactt gattgtttgt aaaccttatt tatctataat taaagcttat
    37261 tatagtataa gaaaatttac ttttaaagaa tattagaaaa tatttgtata aaaa
//
LOCUS       DAFKMX010000015        36705 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFKMX010000015 DAFKMX010000000
VERSION     DAFKMX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.015"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..1924)
                     /locus_tag="LZB08_000640"
     CDS             complement(<1..1924)
                     /locus_tag="LZB08_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314155.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIDKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRK"
     gene            complement(2107..3582)
                     /gene="spoIVA"
                     /locus_tag="LZB08_000641"
     CDS             complement(2107..3582)
                     /gene="spoIVA"
                     /locus_tag="LZB08_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBM7314156.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIVQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3900..4919)
                     /locus_tag="LZB08_000642"
     CDS             complement(3900..4919)
                     /locus_tag="LZB08_000642"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBM7314157.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(4950..5564)
                     /gene="plsY"
                     /locus_tag="LZB08_000643"
     CDS             complement(4950..5564)
                     /gene="plsY"
                     /locus_tag="LZB08_000643"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBM7314158.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLICLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(5599..6918)
                     /gene="der"
                     /locus_tag="LZB08_000644"
     CDS             complement(5599..6918)
                     /gene="der"
                     /locus_tag="LZB08_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBM7314159.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(6952..8280)
                     /locus_tag="LZB08_000645"
     CDS             complement(6952..8280)
                     /locus_tag="LZB08_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBM7314160.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKTLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(8518..8718)
                     /locus_tag="LZB08_000646"
     CDS             complement(8518..8718)
                     /locus_tag="LZB08_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314161.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(8720..9652)
                     /locus_tag="LZB08_000647"
     CDS             complement(8720..9652)
                     /locus_tag="LZB08_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBM7314162.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(9697..10629)
                     /locus_tag="LZB08_000648"
     CDS             complement(9697..10629)
                     /locus_tag="LZB08_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBM7314163.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(10684..11973)
                     /locus_tag="LZB08_000649"
     CDS             complement(10684..11973)
                     /locus_tag="LZB08_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314164.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYISKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(12002..12694)
                     /locus_tag="LZB08_000650"
     CDS             complement(12002..12694)
                     /locus_tag="LZB08_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314165.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(12709..13470)
                     /gene="pgeF"
                     /locus_tag="LZB08_000651"
     CDS             complement(12709..13470)
                     /gene="pgeF"
                     /locus_tag="LZB08_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBM7314166.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVHEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(13467..13928)
                     /gene="nrdR"
                     /locus_tag="LZB08_000652"
     CDS             complement(13467..13928)
                     /gene="nrdR"
                     /locus_tag="LZB08_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBM7314167.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(13950..14213)
                     /locus_tag="LZB08_000653"
     CDS             complement(13950..14213)
                     /locus_tag="LZB08_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBM7314168.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(14317..15090)
                     /gene="sigG"
                     /locus_tag="LZB08_000654"
     CDS             complement(14317..15090)
                     /gene="sigG"
                     /locus_tag="LZB08_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBM7314169.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(15182..15904)
                     /gene="sigE"
                     /locus_tag="LZB08_000655"
     CDS             complement(15182..15904)
                     /gene="sigE"
                     /locus_tag="LZB08_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBM7314170.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(15929..16801)
                     /locus_tag="LZB08_000656"
     CDS             complement(15929..16801)
                     /locus_tag="LZB08_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBM7314171.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(17091..18365)
                     /locus_tag="LZB08_000657"
     CDS             complement(17091..18365)
                     /locus_tag="LZB08_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBM7314172.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(18649..19806)
                     /gene="ftsZ"
                     /locus_tag="LZB08_000658"
     CDS             complement(18649..19806)
                     /gene="ftsZ"
                     /locus_tag="LZB08_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBM7314173.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(19996..20349)
                     /locus_tag="LZB08_000659"
     CDS             complement(19996..20349)
                     /locus_tag="LZB08_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBM7314174.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(20448..21122)
                     /locus_tag="LZB08_000660"
     CDS             complement(20448..21122)
                     /locus_tag="LZB08_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBM7314175.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(21127..21825)
                     /locus_tag="LZB08_000661"
     CDS             complement(21127..21825)
                     /locus_tag="LZB08_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBM7314176.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(21848..22588)
                     /locus_tag="LZB08_000662"
     CDS             complement(21848..22588)
                     /locus_tag="LZB08_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBM7314177.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(22858..24084)
                     /gene="murG"
                     /locus_tag="LZB08_000663"
     CDS             complement(22858..24084)
                     /gene="murG"
                     /locus_tag="LZB08_000663"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBM7314178.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(24102..25232)
                     /gene="spoVE"
                     /locus_tag="LZB08_000664"
     CDS             complement(24102..25232)
                     /gene="spoVE"
                     /locus_tag="LZB08_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBM7314179.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(25284..26639)
                     /locus_tag="LZB08_000665"
     CDS             complement(25284..26639)
                     /locus_tag="LZB08_000665"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBM7314180.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(26658..27626)
                     /locus_tag="LZB08_000666"
     CDS             complement(26658..27626)
                     /locus_tag="LZB08_000666"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBM7314181.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(27667..29040)
                     /locus_tag="LZB08_000667"
     CDS             complement(27667..29040)
                     /locus_tag="LZB08_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBM7314182.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(29207..31186)
                     /locus_tag="LZB08_000668"
     CDS             complement(29207..31186)
                     /locus_tag="LZB08_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBM7314183.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(31198..31545)
                     /locus_tag="LZB08_000669"
     CDS             complement(31198..31545)
                     /locus_tag="LZB08_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314184.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(32026..32961)
                     /gene="rsmH"
                     /locus_tag="LZB08_000670"
     CDS             complement(32026..32961)
                     /gene="rsmH"
                     /locus_tag="LZB08_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBM7314185.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(32991..33737)
                     /locus_tag="LZB08_000671"
     CDS             complement(32991..33737)
                     /locus_tag="LZB08_000671"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBM7314186.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(34131..35417)
                     /locus_tag="LZB08_000672"
     CDS             complement(34131..35417)
                     /locus_tag="LZB08_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBM7314187.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYINYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDVTFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(35432..36697)
                     /locus_tag="LZB08_000673"
     CDS             complement(35432..36697)
                     /locus_tag="LZB08_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBM7314188.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        11162 a         6952 c         3651 g        14940 t
ORIGIN      
        1 tttttctagt atcaggattt acaattaata tagaaccaat cataattggt gttagtatac
       61 aaaataaaaa aataatatct ttttttagat tatattcttt agattctaat ttataattat
      121 tatttattat tccatcataa tcacttatat ttgacttatt tttatttgta tcttgatttt
      181 tgttagaaat actttcccta taaggaaaag aatgttcatt ggtaccaaat actgattttg
      241 agcttaaatc ataattatca tcattttctg taattgaatt tttattgtaa aatccttttt
      301 ctttatcaga attattttct ttattaccgt ctttaggtat atcatgagga tttttatttt
      361 cattgctatc agtacttgat tctacatcat ctttaggagt atttggttgt ttctcatcag
      421 aatcatcatt ttcattatta ttattttcat tattattgtt ttgattgtta ttactatcat
      481 tattttcatt tccattattc ccatcttgat tgttatcatc ctcatctcta tccgggtcgt
      541 ctctacctcc tcctccacgg tcaccatcaa tatcatcatt ttcccgttca tcatctggtg
      601 tctcttggtt gtctccgcct cccattataa atcctggttt aaaaactata tttgaatacc
      661 cttcttttaa tcctcctaca acttttactc taaaacaacg caacttaaaa tcttcaaaaa
      721 aaagtatagc ttgatctcta atatcctcct gttcttcaga tatccaattg attccatcgc
      781 ttgaatattc aacaaaaacc ttacttgcac tatacggcat atcataaaat aaaacagcta
      841 catatccatt ttttgtattt tgaaattcca gttccaaatt gtctattggt tttttatcta
      901 taacagatac attcaaaata ggaactacat catcatttat tataattttt tctttattta
      961 acatttctgt tttaaacttt cctgttatat taaaattttc tcccttttcc aacttttcat
     1021 aataatcagt aaaatcccat tctacatcta tatcaagtcg attaccacta gatgtttcta
     1081 ataccatata tttgggaaat tttatatttt ctattttatc atctttactt acttttattt
     1141 catcactaaa tacaccaatt cccctatgac aacatttcac aatattgtag ccagaatctt
     1201 cagctataga aggtataagc tcattataat catcaattat ataaactttc ttactttctg
     1261 atataattga ttgtgccaac ttattattat cttttagtaa cccactttca ttgttgctac
     1321 tatatgcttt tattgaagtt tcttctatct caacatcacc tttagaataa acacctatag
     1381 catgttctcc attcacttca atatccttat ttagtattct aatatcattt tcagtataaa
     1441 ttcctattgc atctttccca tttgccttta ttttttcaac agattcgtag gatgatttaa
     1501 tatgtaagtc tcctccttct tcaacgtata aggctgtccc atcagtagca attatgttgt
     1561 ttataccaat acttatactt ccaccatttt ctatatgtat tactccttct tttcctcctt
     1621 cacctattat attcaattca ttatcatcaa taataaatct cccatgattt tttatcaata
     1681 ttttatattt gttggtatct attgtaaaat tactgtcata aggaatataa aatctaaact
     1741 cttcatctat taccatatca caagaaagag tataaactat ttcttcttta ttatttttat
     1801 tttcttgaac ccacctttca aaatcttcaa gacaattttc ctcttgtcca aaaactttat
     1861 gtggaaaact tataaaacac aaaactatta tactcataaa aataaatatc tcatactttt
     1921 tcatcttaca ctcctagaaa tttttagctt tgaaacaatc ctgtcgaaac atatacttca
     1981 cccactataa tttttccaca attatcaatt attgtcaatt tatataagta tctttattaa
     2041 ttgttataaa taaaaagcta cttactaaag caaacagacg tttcacttta ataagtagct
     2101 atatttttat aacaaaatag ttataatatt agaacttcct tcatttacaa ttttttgtaa
     2161 agtcttttgc attttaactc ttatctcttc tggcatagta tataatttac tttgtaattg
     2221 ctcttttact aagtcatgta gtgacttacc aaacatattt gattcccata aatctgctgg
     2281 ttgctcttca aatacttcca taagatattt tatcatctct tcaccttgat tttgatttcc
     2341 aactattgga gaaacctctg tagatatatc tgcttttata atatgaagtg atggtgcatt
     2401 ggcttttaac ttgataccat attgttttcc ttgtttcatt atttctggtt cttccagact
     2461 aagttcttct aaagaaggag ctactactcc atatcctttt atcttggcat ctattaaagc
     2521 actttctatt ttatcatact catttttaac tttagatagt ctagttatca gactaagcag
     2581 ttgataatct ccttcaattt taaatccact tttttcttca aggacattat agaataaatc
     2641 ttgctttgtc tgtaaatcaa tatttataac accttctcct agctctacat tatcaacacc
     2701 tgtatcttcc aaaaactcta actcagaaaa tccttgtaca ataccttcta tatcccttat
     2761 tttacctata tctataatac tttgttttaa tgttgttatt atgctacttt ttatccaatg
     2821 atttctttca agaccttcaa cccattttgg taagttgatt cttatttctg taagtggaaa
     2881 atcatataat acagcttcca taacctcctc tatatcttct tcttccattt caaccacatt
     2941 cattggaaga actggaactt catacttttc ttccaattca cttctaagca tactcgtttc
     3001 ctcacttttt ggacttaacg tatttaaaac cactgcaaat ggttttttaa gatttttcaa
     3061 ttcttgtata actctctctt ctggttctac atagcttttt ctgtcaattc cagtaactga
     3121 accatctgtc aagacaacta tccctattgt agaatggtct tttataactt ttttagttcc
     3181 tatctctgct gccttttcaa atgtcatagc ttcttgtgac caaggagtgg atactaatct
     3241 ttgttttcca ccttcctcat gccctaaagc tccttcaact atgtatccaa cacaatctac
     3301 cattcttact tttaaagata ctgtgtcttt tatttttatt tctacaccat ctgctggtac
     3361 aaattttggt tctactgtca ttatcgtttt acctgaacca ctttggggta tctcatctct
     3421 tgttctatcc tttttaaatt cattgtctat attaggtata accaactttt ccataaattt
     3481 tcttataaaa gttgattttc ctgttcttac aggtccaact actccaatat atatatcccc
     3541 ctgagtcctt ttggatatat cttcgtatat gttattattc atattattcc tcctatacat
     3601 ctaatcttat taaattatat ttagagttaa gggttaatat tcttaaaata gaaaaataaa
     3661 gactagcaat attaatttcc cctatataca aaaactagta aactacaatg ctaattgaaa
     3721 ttattaaaat ccattacatt aaaagacata attatagtca tgataattac aaattatata
     3781 taaaataatt tattatatcg tatatacata gactatgcta agtaaaatta aaatagccct
     3841 ctttatatta aagagggcta aaacaaatct attatttttt tattttatta tacaatagtt
     3901 tatatatcat cacctaaaac tacttcttcc atttcatgct ttttacttct catcattagt
     3961 tctattccaa cttctttagg atttgaacct ttctttatta cagaatatat agcattagtt
     4021 ataggcatat ctatatctaa tttttcagca acatcatgtg ctacttcagt agcagttatg
     4081 ccttctacaa ccatctttac ttcttttaaa gtatcttcta aactcatacc tttacctatg
     4141 agtataccag ctcttctatt tctactatgc atacttgtac aagtaactat taaatctcca
     4201 atacctgaaa gacctgcaaa ggtagacata ttagctccca tagcaattcc aagtctgctc
     4261 atttcactaa tacctcttgt cataagggca gcttttgcat tatcaccata ccctaatccg
     4321 tcacatatcc cagctccaaa tgctataata ttttttaatg ctcctccaag ttctactcca
     4381 actatgtctg gattcgtata aactctaagt ttagggctca taaataaatc ttgtatcatc
     4441 tgtgctattt ttaaatcttt agaagcaact actacggtag taggaatatc ccttgctact
     4501 tcttcagcat gagatggccc agatagtatt acatatggat tttgtggtaa ttcttcttca
     4561 cagacttgag ataatctaag cccagtccct ttttcaagac ctttagctac attaacaata
     4621 acttgtcctt cttttataaa tggttttatt ttttggcaag tacttcttac agcctgtgat
     4681 ggaactgcta aaactattac cttacaatct tttatagttt tttctatatc agtacttata
     4741 gttatattat ttggaaataa tactcctggt aaatagtctg tattttcttt agttctgttt
     4801 atttttttag cttgttcctc ttttcgagtc cacatgctca catcattacc tttttttgca
     4861 agccctaaag ctaatgcact tccccagctt ccagtaccta atacacatac tttctccatg
     4921 tttaacacca cctgagtata atatatttac tattctttct tttgaccaat ttttctctct
     4981 gtcccattta ataatcgctt tatatttgct ctgtgattat atattactga tgcagttaag
     5041 aacagagcta ctattaaacc agctttattt tttaccataa tcataaatat tggtgaacat
     5101 cctattccta caacagaacc caatgaaaca tactttgtta tagctaccac caatataaat
     5161 actgctagac atattagtgt tattactgga tttacagcta gcattgcccc tagtgaagtt
     5221 gcaacacctt tgcctcctct aaatcctaaa accgctggcc aattatgacc tgctacaaca
     5281 catataactg ccagatatcc agctaaaaga gtatctattc caaccaatct tgctgcaaat
     5341 tctgatatta aaacagccat tacaccttta agaacatcac ctaaaaatgt cattgctcct
     5401 gctctttttc caagtgtcct aagtacattt gtcgaacctg cattaccaga accttgagtc
     5461 cttatatcaa ccccagcaat tcgctttgca actatgtatg aggtagatat atttcctaaa
     5521 agatatgcaa ctacagcaat aattatataa ctaaatatct ccactgaatt tccccccttt
     5581 agttatattt taagatattt attttttatt tttttgtcta tactcaaact ttatggaagt
     5641 tccttcaaat ccaaaatttt ctcttatctt attctcaagg tatctttgat atgagaagtg
     5701 agttaaatcc ttatcattta taaacaatgt aattttagga ggtctaatgt cagtttgagt
     5761 cccatagtaa atttttaatc ttctaccttt gtcagatggt ggttgattta acattacagc
     5821 ttctccaatt acttcattca gtgctgaagt tgatattctc ttagaatgtt catttgacac
     5881 atattcaact gtatctagta tcttgttcat tctttgatta gtttttgcag aaacaaatac
     5941 gattggagca tacatcataa atggaaattt ttcttttata tcttttgtgt aattactcat
     6001 agtcttatta tctttctcta ttaaatccca tttattgatt acaaatatgc atccttttcc
     6061 ttcatcatgt gctatacctg caacctttgt atcttgttct gttacacctt ctaaggcatc
     6121 tattactatc aatactacat ctgctctatc tacagcactc atagctctta ttacactgta
     6181 tttttctatt gtctcatata ttttactttt tcttctaagc cctgctgtat ctattagaag
     6241 aaatttctgt ccattttttt caaaataagt atcaattgcg tctctagtag ttcctgcaat
     6301 tggacttaca attactcttt cttctccaag tatcttattg agtatagaac tttttcctgc
     6361 atttggttta cctgttatag ctactcttat tatatcttct tcatactctg tatctaatcc
     6421 tgctggaaaa ttttcaacta tctcatctaa taaatctcca aatcccatac tattggctcc
     6481 tgaaactgca aaaggcgttc caaatcccaa ctcataaaaa tcgtatatat tatcaaattg
     6541 actttgacta tcaatcttat taaccactaa gattactggt ttttttgtct ttctaagcaa
     6601 ttgtgcaatt tctttatcag cagctgttat tccagcttta ccatcaacta taaaaagtat
     6661 tacatgtgac atgtccattg caagcatggc ttgatttctc atttgagata atattatatc
     6721 ttcgctatca ggttcaattc ctcctgtatc cactaatgta aaatatttat ctaaccattc
     6781 tacttcagca aatattctat ctcttgtaac tccaggtgta ttttcaacta ttgatatcct
     6841 ttttcctgca aatttattaa atattgtaga cttaccaacg tttggtctcc caactacagc
     6901 tacaactggc ctacttatac tcattttatt tctccttcca tcttttgttt tctattttaa
     6961 aattttatcc acaaaatctt ttccttcatt caaacaaggg ataattttta ttctcataag
     7021 ttcttctaat tgatttaatt caatattatc taagaaaatt tcttcatctg cttttagcat
     7081 acttcttgtt atataaagag cttctcctaa ttctttatct tcaagttgtt cttttaaatc
     7141 cttagctgtt aaaagacctg atacagttat tgtctctcca aaaaacttgt ttttgatttc
     7201 atatacattt acttgaagag ttttaaactt gtccatcata gcatctgcca tactctgtat
     7261 aaattcatat gcagaatgac cagtagctat tgatactttc ttctctcttt ttagaatatt
     7321 ctcaggcaat atatttagat actcttcaat ctctgttttt aacttacgta tcatacctac
     7381 accatcttca aactgtaaaa aaccttcata ttcttcatat ccaggcaatt cactattaga
     7441 tagtatataa aattcatctg ataaaaatgc aaatcgagta cctaattttt caagatattt
     7501 ctgttgtatt tgatgtattt gctctatagt tttacttgcg ctcttatcat taaatatatt
     7561 taattctact agattttctc tatgctttgt aatcccaact ggaactatcg caacactgtt
     7621 tacatacgga taaagttgag ctagttcttt tattgttcta tccaattcct ttccatcatt
     7681 tatacctgga cataaaacaa tttgacagtt catttctata tgagcatctg ccaatctctt
     7741 cattatacca tataattttc ctgcaaattt attgcttatc attttttgtc ttaactctgg
     7801 attagttgta tgcacagata tatttatagg acttattcta tattttataa tattatttat
     7861 atcttcttca ctcatatttg taagtgtgac aaaatttcct tgtaaaaaag ataatctcga
     7921 atcatcatct ttaaaataaa gagtttctct cataccttct ggcaattggt ctataaaaca
     7981 aaacatacac ttatttctac agcttttagc ttggtcaatt atcggatttg taaattcaac
     8041 acccaaatct tcatcatagt ctttttctat ctcgtatatg tagacttcgc catctttttt
     8101 ttgtatttct acatctaaat attcatcact tagtaagaat ctatactcta ttatatcatg
     8161 tattggctgt tcattcacag atataagtaa atctcctacc tctataccca tttcttctgc
     8221 aatactatct ttataaacct tacttataat attatttact ttttttacat ttacttccaa
     8281 tattttcacc actcttttgt attcatcaat gcttattata atgtatttta atatgcaatt
     8341 tatttattta ttatatttta tgatagcatt tttaagcatt tcatgtcata ataacacatt
     8401 ttaattctaa tagtcaatat aagtaaatag ctataaaata gattagaaca aataatattc
     8461 aaatttaagt ttcttaaaaa tttaaatatt atttgttctt aaaaaaattt tataaattta
     8521 atgttttact atgattaacg tcccatttgt catatcatga accattccac ctacacactt
     8581 tgacatcaac aatgccttat tttgtaagtc tttctcatca tttgcataaa atattggaca
     8641 acctccacca tttttcatat tcttatcaag tgtaactata gctagggtat attcatttaa
     8701 acctatatcc attcccatct tatgcacttc ctttctttcc tattttataa ttttttagtg
     8761 ataagttttt tcctttagag cttgaaagta ttgggcatga ctttacagct tccattactt
     8821 tttccacatt cttatctact ggtaaataag caatcattat actttcatcc tctttatttc
     8881 ttctaggtaa tggtacgaat gaaggttctc cataatctcc atataaacct aaccttgaat
     8941 atatattgta tactattgct tgtctttgac cggagtcaaa taatattcct gcattagcat
     9001 aatccttatt tttaggaatt atttgtattc ctacacctct attaatatat acttctctat
     9061 cgctttttag acctatattt gttatacctt ttaaatctcc cacctttagt atagattcgt
     9121 ccacaaaact aatttcagca ggcattacat cagctatgtc tccaatactt tttctagtaa
     9181 gaagtttttt taatataaag gctaacacta atccacaaat tgaacttatt accaaactca
     9241 tcccttcact tattttcgtt tctgcaagta ttacataata caatcctatt gttaaaaaag
     9301 atgtaactat acacatataa tttcttactt cataggttct agctatctct tctatgaatg
     9361 cagctcctct ctttacaagt tgaacttcct ctaaattttg caatgtattt cttctatttg
     9421 ctctcacttg tctaaattgt tctgctgcta gagataaaaa tgttatggat gtataagaac
     9481 gatttataag agctggaacc aataatgctc ctaatgaaga agcgacgaat gctaatataa
     9541 gttgagatag taaaacattt ggttgcgttg gatactgttt ttggtctaaa ttcaacatta
     9601 taattctaga tgttatacca ataataacag caacaaaaaa tactctatcc attaaatcac
     9661 tcctaaaata tttaatttga aattttacat gtaaatctac aaagtctttc tagaaaaatc
     9721 actctgcttt ccttttgatg tttctatgat aggtgtactt ttcactacat ctatcatgac
     9781 atctatatct ttgagtattg gcatatatgg tataaccaca gtactttttt ctaaattggt
     9841 cttagatata gcaacaatat caacctcatc tacatctcta tctatcccca tatgaataaa
     9901 tagattatgt attatagctt gtctttgccc tatatcattt actattccaa atgcacctaa
     9961 atctcttggt ataacttcta tagccagaca ttcatttcta tacttttctc ttgtactttc
    10021 tagacctatg ttagttatta ttacaccatt taccattaaa ataggtcctt caaaacttat
    10081 ttttgcagga actatatctg ctatatctgc tatactgttc cttcttaaaa atcttctaaa
    10141 aatcaaacca acgatagctc ctgacacaat agctaagatt gtgcaataaa aaaagctaag
    10201 gccatacttt cttgcaaaaa cgatataaac tatcgaagat accagtgcag aaaaaagact
    10261 tatataactt ctagattcat acgtagatgc tatttcttca acgtaagcgt ctccttttgg
    10321 tactaattct tcattatcga tattctttag tgttattctt tcttgttctg caagaccttg
    10381 aaactgttgt attgctactg caaaaaatgt caaggctgca aactccttat ctataagtgc
    10441 aggtaatgct atagcaccta acgaagctga aagcccagat ataattatct gttctaaata
    10501 atcttgtggt cttgatgggt attgtttgtc tgtaactctt aaaactaatc ctctacataa
    10561 tattcctaca actaatgcaa ctataaaaga gtgtctaaat aaatttgcat tcaataaact
    10621 ttcttccatt ttctcagctc cttctcttat atagtttgtg caaaaaacaa aaaaaattcc
    10681 cttctattta aggaaatttt tttgtaatat tgtaactata aattcgctac caatgccaag
    10741 ttcacttttt acttttatac atccatttag tgatttgact atatgctttg ttatagccaa
    10801 tcctaaaccc gttcctccta catccctact tcttgcttta tctaccctat aaaatcgttc
    10861 aaatatgcgt tctacatctt cttttggtat ccctacacca ttgtctataa ctttaattac
    10921 gattttttct tcatctttta acactacaac ttgtacaatc ccattatctt gggtatactt
    10981 tatagcatta tcaactaggt ttaagaacac ttgctttata tagtctttat tggatagtat
    11041 acttattgat ttatcatcac atttatataa aacttctata tttttttcac tagctgaatg
    11101 ttgagttatt tcatatacct ctttaaatac atcatatact acaacatttt ccataagaag
    11161 agtatcttta ttttctacaa atgataacaa caatatatca tcaatcaacc tttttaatct
    11221 atctgattcg ctttctatta tttctaagaa tctatttcta gtattcacat ctatattttc
    11281 atttgacttt agtgtttcaa caaacccact tatagatgta agtggtgttt taagttcatg
    11341 acttacattt gcaacaaaat cacttctcat attttcaagt ttaacttttt tagtaatatc
    11401 ttctatattt actatcgaac ctattattac gttgttagta ttctgaagat aaactgggtc
    11461 tacttttatc ttatatacga tatcatcttt agtagtaata ttagcagttt cattatcttt
    11521 agaaccaata aattgaagta tttgttttaa tatttcttct tcttttatta caacattaat
    11581 attatgacct tcaactaatt gatttggttc actctttatc atttcttttg cttcatcatt
    11641 tatcagaagt atatttccat ttacatctat agctaaaatt ccatgagata tactctttaa
    11701 tatagacctt aactgtaaat gtttatactg tacttcttct aaggttttat ccatattctt
    11761 tatcatatga ttaaaatttc tagctaattc gccaagttct cctttggctg atatgttaag
    11821 cctagcatga tattcattat tacttacttt ttttgagatg tatataaact cttttaaata
    11881 tttccttaat gttattgtgt atcttacagc taatataaca atggctatgg acaacataac
    11941 aataaaaaaa tatatactat ttaaatcttc cacaagataa cactcctaaa atataacctt
    12001 tttaatctat tttgtatcct acacctctta tagtctctat atatttttcc gatttatctt
    12061 catcttctat tttttttctt aaatatctta tatgaacatc tactgttcta gtttctccat
    12121 agtattcata cccccatatt ttatccaaaa gataatttct tgaaagtact tttcctttat
    12181 tttgaattaa taattttaac aattcaaatt ccttaagagt taactctatc ttttcagagc
    12241 ctttggtaac ttcatgtttt gataaatcta gttttaaatt acctgtagta agtatattgt
    12301 tttcttcacc taagctagtc aaattatatc tccttaaaac agaactaatc ctagctaaca
    12361 actcttttat actaaatggc ttagttatat agtcatcagc acctatttca agcccttcaa
    12421 ccttgtcatt ttccatattt ttagcagtaa gcattataac aggaatattt tttaagtcct
    12481 tatcacttct tatttttctc aaaacttcta ttccacttat atttggtaac atccaatcta
    12541 aaagtattaa atctggttta atttccttag cctttataaa tccgtcaaat ccatcatatg
    12601 aataactaac ttcgtaattt gacacctcca aattgaattt taatagctca actatatgca
    12661 tttcatcgtc tataacaagc actttagtat tcattaaaaa gctcctctct attttatctg
    12721 tagtaccgtt cctattcgtt ctgatgcctt attatgtttt ctataagagt ggaatttatc
    12781 tgctctacaa ctagtgcata aatttaaatt tattatattt tcttttttaa ctccactaca
    12841 tctaagcata tactcattaa ttttccataa atctaaatag taacttccct cttttattat
    12901 atagaacttt tcatcattat ttgtaagaat tgtgttaaat ttttctacta aatcttcaga
    12961 cacttcataa caacatggtc caattgatgg cccaattata catactaaat cttcatgaac
    13021 tgttttatag cattcagaca ttaattttat tgttttatga cctatattac taaaagtccc
    13081 tctccaacct gcatgagcca gtcctatggc tttattttta gcatctataa ttgatatagg
    13141 tacacagtct gctgtaaaca gtaaaagtgg tactttttcc aagtttgtta taagtgcatc
    13201 tccatctctt cttttcccta tagtatcttt attgactata ttaactatat ctgaatgtat
    13261 ttgagaatta ctagttagat tttcaaatgt caaatcacat tctttacata cttttaacat
    13321 atcttctcta ttttttgcat ctatattctt acatgttata actagattta cagaatttaa
    13381 tttttcatca agttcttcac aattaccact taaattaaca tactctttaa tatcttgtac
    13441 attttttata tttatataat ctttcatcat ttgtctcctt tttctatcag tatactttta
    13501 agttcattta caaaagtatt tatatcttta aattgtctat acacagaagc aaatcttaca
    13561 taggcaacct catctaactc tttcaattta tccattacca tctcaccgat ttttttggtt
    13621 tcaatttctt ctataaaaca tttatttatt tcattttcaa tatgtacaac aatactttca
    13681 atctcgtcaa tagaaacagg tcttttttca catgctttta gcaatccata ttttatcttt
    13741 tctctatcaa aatattctct actattatcc tttttaatga ccattaaagg agttgtttct
    13801 attttctcat acgtagtaaa cctcttttta catgattcac actcacgtct tcttctgata
    13861 gattttaaat ctgtatgtct agaatctatt acctttgatt ctttataatt acaataagga
    13921 cattgcatct atattcctcc tttgtttaac tatatctcaa actcatctaa gcttaattct
    13981 gaacctatat ttactattat tgtatcacaa cctattttaa gtatatcatt ccaaaatata
    14041 acttcttcat cccctcctct tgaaaaaaaa cttctacttc catctccaaa aatggtaaat
    14101 gagactacct taccttcgtg aatatccatg tctatgtcta ttataaaccc catccttttt
    14161 ccatttttta catttataat ttctttttcc attatatcag aaactctaat catattgtac
    14221 tcaccctttc tttaaaaata aatatgtatc gaagtaaaaa aaaatgacct atataggtca
    14281 tttaaattaa aataatgttt acagtccatg gattacttat acatattttc tcatattctt
    14341 taaagcattt ttttcaattc ttgatacttg tgcttgagat ataccgattt catcagcaac
    14401 ttctatctga gttcttcctt tgtaaaatct taaatctaat accaactttt ctctactatt
    14461 taatttctta atagcttctt taagtgatat ttcttgcaac caattttcat cagtatcttt
    14521 tttatcttga acctgatcca taacgaatat tgcatctcca ttgtcttgat atactgggtc
    14581 aaacagagat attgggtctt gtatggcatc aagtgccatt acaactgatt ctacttctaa
    14641 ttctaattcc tttgctattt ctgatactgt aggttcttta gaatttgttc taattaatct
    14701 ttctctaact tgtagtgcct tatatgctat atcttttaat gacctactta ctctaattgg
    14761 gttattgtct cttagatacc ttctaatttc tcctataatc ataggaacag cataagttga
    14821 aaaccttaca ttttggctta aatcaaagtt atctatcgct tttataagac caatacaacc
    14881 tatttgaaat aaatcgtcta tattttctcc tctgttgtta aactttttta ttacactcaa
    14941 gactaatctt aaattccctc ttacaaactg ttgtctggct tcttcatcac cattttttat
    15001 ttgtaataaa agttccttca tttgcttatt tttaagaact ggaagttctg atgtattaac
    15061 accacatatt tcaaccttat taacttgcat atattttcag tcctctctca aaagtattca
    15121 tctattgaaa ttatttacat ttccaatttt tttatacttt ttaagaagaa ttaatttagc
    15181 attatacaaa ttttttcatt tctttttgca accttgagat tattttcttt tcaagtctag
    15241 atatgtagga ctgtgatatt ccaagcattc cagcaacttc tttttgtgtt ttttcaatac
    15301 ctttatcaat aagaccaaat cttaattcca taatttgctt ttctctgtca cttaatctgt
    15361 caagtgccat aaccaacaaa tctctatcta tttcttcttc aattattttg tatatttcat
    15421 cattttctgt acccaataca tcagataata aaagttcatt tccatctaaa tctatattta
    15481 aaggttcatc aaatgatact tctgttttct ttttattatt ttttcttaag tacattaata
    15541 tttcattttc tatacatctc gaagcataag ttgccaattt tatattttta ttcagtttaa
    15601 aagtattaac tgcttttatc aaacctatag ttcctattga aatcaaatct tctacatcta
    15661 tacctgtatt ttcaaatttt ctcgatatgt agactactaa tctaagattt ctttctatta
    15721 aaattgactt tacactttca tctgtttcta gcttttgtaa aagctccatt tcttcttctg
    15781 gtttcaatgg aggaggaagt atatttgccc ctcccatata gtatattccc tttggcttta
    15841 ttatttttaa tttaatacct aacatagtaa taaagcttat tattctttct ttcaatcgta
    15901 acattccttg tcccccttaa attttcacct aaaatccagg caatatattt ggattcatta
    15961 ttgctccata ttctctatca ttaaaatttg aaataccaag tattatattt cctatctttt
    16021 gctcatctac ttctatatag tcagctttta agcctattat tatgcttgtc ttattacttc
    16081 cagcatgttt ataaggtatt attctaactc tacctgaagt tttatcatct aaacttccta
    16141 taatttcttg tgctttcttc atatctatat cttcataatt aatatttaat agtttttctg
    16201 gaataaatcc ttctaacaca ctagacttaa ctattattac atcactttga cttattgggt
    16261 cttttaacaa attaccacta tctactaaag ctttacagca aaaacttttt tctagtaagt
    16321 ttatatttat agtctttgta agctccttaa tgtacttaag cattttcaaa tctttataaa
    16381 tgtattttaa taattcacaa ctcacataag tacatgcaat taagaaagat atttttaagt
    16441 gacttattcc tgtaaaataa attataaaat aagtgcttcc agaaataaaa atgtttacta
    16501 gataaaaaac taacgctaca tgaacataat cttttttgct cttacttcta aatgaaatta
    16561 aagttattat actcatgatt attattttaa atggtacagt aaatagtata ttcattgatg
    16621 gatacaaata agcaacagaa taggcacctc ctaaactagc ccccaataac ttattttttt
    16681 taggattata tcttttagta agaatagaag tacaacttat aataatataa tttattacta
    16741 ggttttctat aacatagtac tcaatataca ccatgtatcc ccccttaaaa cctttaaaca
    16801 tattataaga tagtatgtac aaaattttat gtcacttttg atggtcgaat ccaaaacaaa
    16861 ttctatttaa aaagaacaat gatatatgtc ttatacttta tgtcgtcaaa agatattttt
    16921 ttaataccaa acaaatagac ttttttgtag aatccaatat atctaggtag aagttattcg
    16981 ctctactttt ggtatattta caaaaaaaga ctacataatt taataatata tcaagtaaat
    17041 cttttactta tataaaatca aattatatag tctgtaaatt caaatataaa ttattgtgct
    17101 agtaatccat ttgcatattt tgcagattca tttagtgctt gttctggagt tatgtctcca
    17161 gctatcatca tttgaagttg tttccataaa tattcatata tctctactcc atgaggtcca
    17221 actgtcaatg gtctatacat atgtttatct cttgtcacca tttcttcaaa tcttgggtct
    17281 ccttggtatt tttcaccttt agcaactgga gctctaggta tttctttgtg gaaagcttcc
    17341 attgattctt tgcttagcat atactcaaga agtttaaatc caagttcttt atttttacat
    17401 gctgacatta atgttaaatc atctactgaa gcaaaacttc caactgtctt atcttctaat
    17461 gatgttacat atccccagtt taaatccggg aatgatttat caaatgtact tgtagcagca
    17521 gaggatagca ttatggtaaa tgcagctttt cctggtccaa atacactttc aatcatttca
    17581 ttactatctt ttgataaagc atcttctgga atatattttt tcatatcatt tataaagttt
    17641 gcagctttca agccttctgc attattaaat ttaactgatt ttaaatcatc attatatata
    17701 tctccaccag cttgccataa atacggatac cagttccagt ttaagcttcc aaatacttta
    17761 gaaccccaac cttgtgcaag accccactgg tcgatttttc catctccatc agtatctttt
    17821 gttgcttttt cacagattct tttaaaatca tcccatgttt ttggaggttt ttctcctaat
    17881 ttttctagta tatctttatt gtagtacaat acgcctggat tagcagcttc tataggtaaa
    17941 ccataaatac cacccatcat ttttgcatca tctaagtata gataattatc tgaagttcct
    18001 gatttttcta aataaggtgt taaatcttct accgcaccca tttctataaa ttgaggaaac
    18061 atctctgcat acatgtatcc tatatctgga ccttcacctg cacttattgc tgttgcatat
    18121 ttttctgcgt agttatccca tggtactatc tgaaattcaa tttcacagtt attttctttt
    18181 tcaaattttt caaatatcgg cttccatgca tctgcgtcat ggtctgttaa tggtgcagac
    18241 catacaacta atttttcttt accctctttg cctttagaat cttctttttt tgaacatcct
    18301 gcaagaattg atactgatag aatccctacc ataactaaag aaagaacctt ttttagttta
    18361 atcatgctta atcccctttc agactttaat ttgtcttttt cctcaaatgt ttacatttaa
    18421 ttcagattta atattaacaa ataagattac tcttgtctaa tcataaaaaa ctatactaac
    18481 tcctataact atattttttt tctataccta ttagatttaa ttcatactaa aaacttactt
    18541 tttaaatttt atcaatattt aaaaatattt ttaagtctag ccacaaacta actatctaaa
    18601 ttgaacaaaa taaaaagaag tctattgact tctttataag cttacatatt atcttcttct
    18661 tcttaggaat gtaggtatct ccatgctctc atcatcatct tcttcaatta tacttgcctt
    18721 tttaggaggc tctatttttt cctcagcaat tacttcttct tcaatttctt ttacagcttg
    18781 ttttactgtt gtatttaatg aagaacgtat gcttgattta ggtttagtat ctaaatctaa
    18841 ggcaccattt tgtaatcctt caaatcctgt cgcgattact gtaatcatta tttcatcacc
    18901 taaatcttct tttatagaag ctccaaatat aacatttgct tctgggtcac aggattccat
    18961 aactaaagtt gatgcctcat taacttcaaa taatcctaag ttaggaccac ctgtaacatt
    19021 aagaagtact cctttagctc cttgtattga tgtttctaga agtggagatt gaatagcttg
    19081 tcttgcagca tcaattgctc tagtttcacc actagcacta cctataccca tatgagcaag
    19141 tcccttgtct ttcattatag tagtaacatc tgcaaagtcc aagtttatta acccttcaac
    19201 tgctataaga tctgaaattg attgtatacc ttgttttaat acatcatctg caactgcaaa
    19261 agcatctaac attgatgtat ttttttgtac tatctgtaaa agtctatcat ttggtatcgt
    19321 tataagagta tctacttttg atttaagttc tgctattcca ccttcagcgt tcttcattct
    19381 tatcttacct tcaaatgcaa aaggtttggt aactacacct actgtaagta tccccatttc
    19441 ttttgccaag cctgcaacca ctggagcagc tcctgtacca gtaccaccgc ccattccggc
    19501 tgttacaaag accatatcag ctccttgaag taactttact atttcatctt tactctcctc
    19561 agcagctctt ttccctactt ctggatttgc tcctgctcca agtcctctag ttaatttttc
    19621 tcctatttga actttatatt cagcttttga tgtatataaa gcttgtttat ctgtatttac
    19681 tgatataaat tcaacgcctt ttagttgagc ttctaccatt ctattgacag cattgtttcc
    19741 accgccacct acacctatta ctttaatttg tgcacattct tctaattcta cgtcaaagtt
    19801 tagcatcata aacccccttc tctcatgatt caaagttatc gtatgtatta attatatcat
    19861 tttcttcttt tattttgtta acctttaagt aacatattcc acaaaaacgt aaattttcct
    19921 ttttaaattt cttacttata ttgtcaaata ttactcttta agtataaaat aatttacgta
    19981 tatattaaat gctctttaat ttttacgttt tttgttcata atattactaa taatatatcg
    20041 tctaattata gataagttat taaacaatct cataccaaaa actaatacag caacatagta
    20101 aagagaaact cctaactttt ctcctatata agtcaataat atagccaata tagcattacc
    20161 tatgaaacca gtaataaaaa ttacattatc atagttatct tcaaaattgg attttatcgc
    20221 tccaaaaata gaatccatta ttgcaagaat agctactgac atatatatgg aataatccat
    20281 agaatatgta aatggaatat agtaacctac tactacacca aatgctaatc ctcctaaaac
    20341 ccataacata aatcttcacc tctttatttt actttgaaac acaactacat tacaatcctt
    20401 tttattaatt ttttttactt tttctttatt tttttgtatt tttcgtttta ttttgcaaat
    20461 aaatccactt tatttccatt attttcctga acttttatat taattccata tatatcctta
    20521 agagatttag catatgattc tggagagttt atagctgcac tcaataaatc tatatctcct
    20581 atagctttta ttataaatgg ttgtccatat gtagtatcat ttactgtaat agtagctcct
    20641 gaacatttta tcttacttgt tgctagtact ctttctccat ttatagaaat tgctcttgct
    20701 ccagcctttt ttaaatcatt taaaaccctc aaaatatcta tatcatgtat aatcaaatcg
    20761 tttggattct gtccgtcttt taactctctc tcactatccc tcatagtaat agttatgcct
    20821 ggccctgtaa cagttgaata accacttaac tctttaactt cttcaagctc tttttgcata
    20881 agtgtttgga tagattcatt tccattcttt tttgcttctt tatattcatt caaagttttt
    20941 ttgttctttt taagagtttc ttttaaattt tttatttctt tttttgtatt ttctacctca
    21001 ctttcaatac tttcagtttg acctaatgtt atgtatactc tatttgggtc tagtgatttt
    21061 atataagttg atatgaaaac gcctaatatg aatgctccta atataactat tttctttttc
    21121 atactattat ttcccctctg ctttgtttat atcactatga ccatttattt ctatattgtc
    21181 ttctttggtc acttttactt tggcattata atatgtttct atttcccaga caataccata
    21241 ctttatatta agtgctgaag ccaatgtatc agaatcacca attgctttta ttataattgg
    21301 ttcttttatg acagtgtcat tcatataaag cttgtcttct tttaaataca tatatgtatt
    21361 agaaactatt ctctggtcgt ttatagatat ggcacttgct tgtgctgaat ttaatttatt
    21421 tatcattgat aataataaat cataattata tagtatacta tcatttttat cagagtttcc
    21481 taagtctttt gtagaattta tctcaatttt tatgcctgtt ccttcaacat ctgtatatcc
    21541 agctaatgtt tcatattttt ctatttctga cttaagtatg tcttgttctc cttcattgtt
    21601 tttgcctcta taagtctcta tatctttctt cacactatta acttctgatt gtagattctc
    21661 tttttctttt tttaaagatt taagttcttc tgtaagttgc tctcctcttt taaaagttgt
    21721 catccctcta ttttctatat taataatttt aagttgtaaa ctaacaagaa cacccaatac
    21781 aatacttcct atgattatac ttttatttat aaatcttttt ttcaatatat cacttcctta
    21841 caattgtcta tccttctgga ctatacactg ccaactttcc atagttaaga tttatagtcc
    21901 ctccattagt atttttattt tgcaggtcaa tcagaatttt acttacccta gatatattat
    21961 aatctaagtt atcatcatta gaaagtaaga tttttatatt acttcttgta agcatttcta
    22021 tattgctttc tttttttaag tttacataat ttatttttct ataaattcct tcttctttta
    22081 aataatttaa agttttaaat acattctcct tagttttata atttttaaat tttatagatt
    22141 tattatcatc aatgctataa tctacatcta ctatcaagtc ttttttactt tcattagaac
    22201 ctcttagttc ttccaataaa tttccttttt tatctatata acaataatta tcttcatctt
    22261 ttaatactgc aaatatttct ttttctttta aacttataat tatcttattg ggaagttttc
    22321 tttttatttc cacattttct atatatggat tttttataag tttatttttc atatctttaa
    22381 aattgtatgc aaaaatattt tcatttagat ttacatttag ttctttcatt atatttgatt
    22441 tggtcaccct cttatttcca attacatcta ttttctttac atcaaataaa tcacttttta
    22501 caattataca tatacaaaaa gataacataa gtataaaaac taaaactatc atgacattat
    22561 tcgtatttaa ctttcttctt tttttcaaat ataatcaacc cctttggtta ataaatacac
    22621 ctttagagct tgtttttcct acaaaaaaat tataatattt aaagtcaaat ttttgcattt
    22681 tattacataa catccaaata aggtattaaa ttttataaat aaatcaatat ttcatcaaaa
    22741 taaacatagc atactacata ctatttatat atttaaatct gtatacatac aaataaaccc
    22801 ctaatttata ataatttaga aaatctaata tattataaat taggggtaaa tatttactta
    22861 tcttttcttt attcctatta ccttagcttg accttctata cttggagtcg tttctttttt
    22921 tatttctttt acttccttaa taactttttc tttttttgtt tttttagaag ttgacttttg
    22981 tgttgaattg tatactttca ttatctcatc atatataagg tctatggctt caggcttacc
    23041 tatagtttta cttgcatttg ccatatcaac caaaagttct ctatctccta aaagtttaaa
    23101 tactgctgta tttaggctct ctggagttag atttttttct aaaatagcaa ttcctgcccc
    23161 ttgtttttct atactcttgg cattatactc ttgatgattt tcagcagtat aagctttagg
    23221 tattattata gatggctttc caagagccgt tatctcagct aatgaaattg ctcctgcgct
    23281 acctataacc aaatcacttg ctgcaagtgc atttgccata tcttctaagt aaggcacaac
    23341 tttttggtat ggtttaagat ttatatcgct aatacttccc ataaattcat cataatagct
    23401 tttgcctgta gcaaatataa aagctatatc ttcatttacc atattcttta ttacaagtct
    23461 cattgcatca tttatttttc ttgaacctcc actaccacca tagcaaagta ccattctttt
    23521 ttcgtcactt attgatagat tctttcttgc aatatttttt cttgataata aaatttcttt
    23581 tctaactgga tttcctgtaa aaactaattt atcttctgct gcctctggaa atcttttatg
    23641 cgaatcttca aaacttgtaa gtacctttgt aactgtttta gataaaatct tattagttac
    23701 accaggaaaa gaattttgct catgtattat ggctggtatt ttccccatag atgcattaaa
    23761 taaaacaggt ccactaacat atccaccagt tcctataact atatctggtt taaatttctt
    23821 aacaatcttt cttgattgct ccaaaccttt aaaaagctta aaaactcttt ttacattatc
    23881 gaaatctatt tttcgcttaa agccttgaac tgttacagtc tttaattcaa atccatactt
    23941 aggaactatt tctgactcta ttcccttttc cgtcccaaca aaaattatct cagcgtctgg
    24001 atgctcatct cttattttgt ttgcaatagc tatagctgga tacacgtgcc ctccagttcc
    24061 accgcctgat aataaaactt tcatcaaatc atcatctcct gttaattaat tttgacatgc
    24121 tttgagatgt ttaacactat tcctacagct cccataaaga tggtgagtga agttcctcca
    24181 taacttataa atggtagtgc tacacctgta gctggcattg atgatgtcgc aactgctata
    24241 tttaaagctg cttgtattcc aatttgtgca cctataccta taacaaccat acaagcaaat
    24301 acattagaag tcttcaaagc tattcttaca catctgtaga ctaaaactac aaatagcata
    24361 ataactatta tacaaccaat caatcctaac tcttctccta ttatagcaaa tataaagtca
    24421 ttttgtggct ctggtatata aaaatacttt tgtttactct ttcctaagcc tagaccaaac
    24481 aatccacctg aacctagtgc atatagacct tgtattacct gatacccctt accaagaggg
    24541 tcttgaaaag ggtctaaaaa tgatgtaaca cgactaagtc tatagggttc agcaattact
    24601 aatgcagcaa ataaagcagc cccactccct atcatagcaa atacaatttt catatccatt
    24661 ccagcaacaa atatcattac aaaagtaact agaattacag tacctgctgt tgacaaattt
    24721 ggctgaagta ttatcaacgc aaaaaatatc cctggaacta cgagaagtgg tacaacacct
    24781 tttgtcaaag attttatttt atcataattt ttttcaatta attttgcagt taatattatt
    24841 gtagcaaact ttgcgatttc tgcaggttga aatgttaaag ctcctattcc caaccatctt
    24901 tttgctccgt ttgcttctat ccctaaaggg gtcaaaacta acaataataa tactactgct
    24961 atagctccaa ttgctgtagc atttttcttc caaaaactat aatctattct tgatgtaata
    25021 atcataacta taaatccaag tacagcatat attacattct tttttagaaa atagtacgca
    25081 tcattgtgtt taaatgaaga ttgtataaaa cttgcactaa acaccataac tattcctaca
    25141 aatactaaaa gcatagtagt gtaaaacact actccatcaa attcagtctt catccttatg
    25201 tcaatttgtt ttttcaaatt ttctttaggc atccaactaa tctacctcca ttcttccatt
    25261 taagtaagtt tagaggtaag agattatttt aaattgttta cattatcttt gaagtctttt
    25321 cctcttactt caaaactttc atacatatcc caacttgcac aagctggtga aagaagcact
    25381 atatctccac tttttgaaat ttcataagaa gtcttaactg cttcttccat atcttttact
    25441 atatgtatgt cattaaatcc tttattttta gcacaatttt ctatatttga tgcagtttct
    25501 cctaataaga ccaatgattt aacatatgac tttgcagttt caagaagttc atcaaaatta
    25561 cttcccttat ccatcccccc tgctataagt attataggtc tatcataaga ctgtatagct
    25621 tttatagtgg aatctggatt agttcccttt gaatcattta catatattac attgtctaaa
    25681 tttcttacaa actcttgtct gtgttccact cctttaaatg tcataagtac atcctttata
    25741 acttctaaat caattttgca gacatatgct attgctatag ctgccataca attttctaag
    25801 ttatgacctc caggtaaact tacatcatct ttatttagaa atttgatttt atcatctata
    25861 tctattatta tgtcattatt ttctaaatat actcctccat ttactttttt agttcttgaa
    25921 aagtaaatta ctttggcatt gcatttatca gctaaagatt ttactatctc atcatcatag
    25981 tttaatacac agaaatcctc aacagtttgg ttcataaata tatttgcctt tgcttctatg
    26041 tatttttcca tagtatggtg tctatttaaa tggtcctctg taatatttaa tattgcgctt
    26101 actttaggtc taaattcatc tatactctca agctgaaaac tgcttagttc agttactaac
    26161 acagactctt cacttgaagt ttctatagta tctattacag gatttcctat attaccaact
    26221 acatatgtat ctcgttttgc tcttgaaaaa atttctccta ccaaactagt tgtagttgtc
    26281 tttccatttg tacctgtaat ccctataaaa attggattat tcgatagttt aaaagctaat
    26341 tctacctcac caattatata cttgcctgaa ttttttaatt tcaatatgaa tggtaggtct
    26401 agtggtacac ctggagacac tacaaccata tctatatcat ctacatcttc tgggtgatgt
    26461 cctaatatgt attttatgtc atttatagat ttcaattcat caagtatatt tctcaattta
    26521 ttttcatctt ttatatcatt tacgattata cttgcaccca atttatctaa atgtttaatt
    26581 gtagatatac cagtttttgc aagtcctact aataaaactt tttttccttt taagtccatt
    26641 tttcattccc cctagcctta aaatactgct attatactta tccaagctaa aacaactgtt
    26701 ataatccaaa aaataaatac aactcttgtt tctggccaac cacactgttc aaagtggtgg
    26761 tgtattggag ccatcttaaa gaatctcttt cttgttgcct taaaatatcc aacttgaatc
    26821 aaaactgata gagcctccgc aaaataaatt cctccaatta taggtattat aagcactgag
    26881 ttagttagaa ctgaaaatgc tacaactgca cctcctaatg ccattgaacc tgtgtcgccc
    26941 ataaacactc tagccggata tgaattaaat cctaagaaac ctaaacatgc tccaacagtt
    27001 gctgctgcta aaactgcgac ttctgtattt ccagcaatag aacttgcaaa tagcataaag
    27061 aatacagaaa ctattaatgt tactcctgat gctaatccat ctaatccatc agtcaagttt
    27121 acagcattta ctattgctac gattataaac atcattattg gtatatataa tattccaaca
    27181 tttatcacaa aatctgtaaa tggtatcata agttgtgaag cacttgatga tgatgtatac
    27241 tgataaaaag caacataaaa agataatgca acttgaagga ttattttttg atatgctttt
    27301 agtccaagtg atctttttag ctttattttt ataaaatcat ctaaaaagcc aataaaacca
    27361 aaacctgcaa tacatataag acctactgcc atatcatgac taacttttac tcttgtaagt
    27421 ccagttatta gaattgcaac aatcattata attccaccca tagtaggtgt tccgttttta
    27481 gctagatgtg tttggggtcc atcatctcta actgtctgac caaatttaaa ttttctaagc
    27541 attggtataa atattggtcc tattataatt actattaaaa acgcaatcaa tgcggtgtac
    27601 gtaagctctg ttattcctaa catcataaaa ctcctctccc ttgtcaatta actcattagt
    27661 ttaagcttat ttatttagat attcgactat tttttctaat ctcatgcctc ttgagccttt
    27721 tactaaaata gtgtctcctg tttttactaa ttcatttaac ttgttaaaca catcttctct
    27781 attctcaaag tgatatatat tggctgaatt gaatcctaat tgttttgctt cttctccaat
    27841 aaacactgaa ttttcaccta tagttattat aatatcagta ttattcatag atgattttcc
    27901 aactaatcta tgaccatact ctgaaatttc acccatttct agtatatctc caagtatcgc
    27961 tacccttcta cctttatatc ttccaagtat ctttaaagca gcatccatag aatctggact
    28021 agcattatat gaatcattaa ttattgttaa tttttcattt tttattatgt ctaatctcat
    28081 ttttgtccct ttaaaatttt ttaaaccttt ttttatataa tctaaagaaa tatttagaca
    28141 caaaccaact aatatagcag acattgcatt atatatatta tgttctccta ttgttggtat
    28201 aaatatttcc tcttttttcc catttattac acatataaat gttaaactat cctcttccat
    28261 agtataactt tcacaataaa catcattgtt tttattaaag ccaaatgttt ttaacttata
    28321 tacatgttct ttttctttta atgtagataa aaacttatca tctccattta caattaaagt
    28381 atttgtttca tcaaagtttg aagtaatctc catctttgct ttcaaaatac cctcttgtga
    28441 acctagtttt tctacatgtg ataatcctat attcgatatg actgcaattt taggatttac
    28501 tatatccact aaatatttta tttcattgaa tccagacata cccatctcta ttacagcaca
    28561 ctcatgttct ttatttagat taaataaagt caatggaact ccaaaatgat tatttaagtt
    28621 tttttcattt tttagtatat ttaactttgc agatatggca gagtaaatca tatctctagt
    28681 agtagttttt cccacactac ctgtaacacc tataaatgga atatcaaatt tttctttata
    28741 gtattttgaa atatctccta gagccaaaga tgtatctttt acctctatta aatttatatc
    28801 cgaactttct aattttattc cattactttc attttttata aaagttttac atccactatc
    28861 atatgcagac ttcataaatg tatgcccatc taaattttct ccaactatgg ctacaaatgc
    28921 attttgggca tttgcctccc tgctatctat aactatatca cttacacagt cattataatc
    28981 tccatatatt aacttcccat tagttgcaag tactaactct tttattgtta agcactccat
    29041 tattaaactc ctcctttaaa accttagcta tattctctta gcaacaaaag gcaatgtata
    29101 ccgttatata tacattgcct acgataactc ttaactttat aattatatac tatatttctg
    29161 tttttttgaa gatgtttatg ggtatatgtt aattatctgt tactttttag ttttcaaaat
    29221 atagggttat acttgagtct tcattaactt ttactccagg ttttgggaat atatccttta
    29281 cctttgtatc ttctggaagt tctatatctg catctaagtc tggttttaac ttcgcatcct
    29341 ccaatgcctt tacagcatct cctattttta gatttcttac atctggcact ttaacttgct
    29401 tcttttcata ctcagctttt tcttcttctt tatatactgg tttaactcct aaatatttta
    29461 aagagtcatt cattatttct ttcactattg gtcctgctgt agtactacca aatgcactta
    29521 ctcctgttgg ttcgtcaaca atagcaagaa ctactatttg tgggtcatca caaggtgcta
    29581 ttccaacaaa tgaacatata tattttcctg gagcatactt accatctata actttttgag
    29641 ctgtacctgt tttaccacca agtctataac ctggtatata cgctatcttt cctccacctt
    29701 cagttacaac tgattctgca atttctaaca tttttttaga tgtttcttta gatataacac
    29761 ttcttacttt ttttggtttt actgtttcag ttatatttcc tttattgtca gtataagatt
    29821 ttaccactct tggttgcatt aaatctccac cattagctat tgatgatata gctgttataa
    29881 gttgtattgg agttactgat atagattgac caaatgatat tgtagccaat tcaactggtc
    29941 ctacattttt ttcgttatac agtattccct tagcttctcc tggtaaatct atacctgtct
    30001 tatccataag accaaatgat tctatataat catacatttt tccaacacct aatctgcttc
    30061 ctaactcaac aaatactggg ttgcaagagt tttgtactgc ttgtttaaat tcttgtgttc
    30121 catgaggtct gtaatgtctc caacatttaa tctttcttcc tccaacagtt acactacctg
    30181 tacatgtgaa tttttctcca tctttaatta ctccttcttc aagagcactg gaagatgtaa
    30241 taagcttaaa tgtagaacct ggttcatatg tatcacttac tgctggattt ctccacattt
    30301 gataatagcc ttttatttta tctttatcat tatatttttc aagttcttct tgataatatg
    30361 gatatattgg cgttcttgag tcattagggt cataatctgg ttttgatgct aaagcaagta
    30421 tatcacctgt tttagggttc atagctattg cagtaacttt ttttgcatta tttaactcat
    30481 aagctttctg tactgctttt tcagtatagt gttgaattac ttcatctata gacaacacaa
    30541 gaccattacc ttgtacaggc tcataatatt tttccattcc ttgtggtatt tctcttcctg
    30601 aagcatcagt acttactata agttttccag gctttccttt taacttttta tcatattgca
    30661 tttctacacc agatatacct gttgcatctg aagaagtatg tcctaacaca tatggtgcaa
    30721 aattcccata tgggtaatat ctttgattat cttctgccac ccatattcca cttaatttag
    30781 catctcttat ttgacttgcc ttatcatcat ctatccatct ttttactttt acaagagcca
    30841 tatttttttt gcttattaaa gcgtaaatat ctttataatc ttcatccaaa atctcagcta
    30901 ccttttcggc tgcttctttt ttatcttcta cttccacagg tttacaccaa acagtatact
    30961 tagttacact tactgctaac tctttcatat ttctatcata tatagttcct cttttgggtt
    31021 ctattggtat atctcttgtt tgttgttcta gtgcttttgt actcaaccaa tttcccttca
    31081 taagttgtag atacccagtc ctaataacaa ggcaaaaaaa caatgcacat gctagaataa
    31141 gaacgagtac cagccttttc ttacttatcc tctttacttt tctcaaatct attcccccta
    31201 gttatccaca tctatatact ttatttgtcc atcttttgga tagtccatat tgagcaattc
    31261 cttagccttt agctcaattt cttttattga tgttttttca gtttcttttg cctttagttc
    31321 ctctaatact atctcttttt ttcttaaatc tttttgtaaa taatagatat cgtacttcat
    31381 ttccgagatt tttgaataac cacatagatt caatattatt actgaaaaag ttgcaatcag
    31441 taaaatgcat ttagctccta tttttttctt atttctcttt ttataattat tctttttttt
    31501 atttttatat tcatttagat tatgtatttt ataatttttt ttcaaattct caccccgata
    31561 gtatatagtt taaattttcc tctatactat atttgttatt aatcatattt cttcctctaa
    31621 aatttcatcg ctaaattaat acactattac acaatctaat tgtatttttt tctatacaca
    31681 acaaaaacct ataaattcac ttatactttt atatgaatct ataggttaga tattaatttc
    31741 tatttatttt tatcacttct agtattttta gataatttct tccataaata taatatgtaa
    31801 ctaactatct actctctgat tcctaagttc tccattatat tacttaatac catctatatc
    31861 tatattttta tatctaaatt ttcattatat atctcaataa caaagaacat ttcttatagt
    31921 gttaattgtt ttttaattgc tttaccagtg caacaaaata tataatactt tttaattaat
    31981 tataataatc ttatatataa gattattctt tagccttata actacttata ttttttctgc
    32041 tactcttagc ttagcacttc ttgaacgtgg atttacttct atttcttcct cactaggtaa
    32101 tataggtttt ctagttatta ttttcacctc agattcttta tcacattggc atataggtaa
    32161 atgctgtgga catatacaat ctgatgctaa atgcttaaat gcatttttca ctattctatc
    32221 ttctaatgaa tggaatgtta ttatacatat tcttccacct tcattcatta tagaagaagc
    32281 atcatttatc atctttgtaa ttactccaag ttcattattt acttctattc gtattgcctg
    32341 aaaagttctt ttagctgggt gaggtccatc tattctggct ttcttaggga tagctttttt
    32401 aattacatct actaattctc ctgtagtttc tattggtttg tttgctcttt cttcaactat
    32461 aaatttagct atacgcttag cccagttatc ttctccataa tcttttatta tcttagttaa
    32521 ttcatcttct gtataagtat ttacaacatc atatgcagaa aactcacacc taacatccat
    32581 tctcatatca agtggtgcat cttgcatata agaaaatcct ctatcagctt catcaagttg
    32641 atgagatgaa actcctaaat cagctaatac accatcaatt ttgtaaactc ctattttttc
    32701 taattcctct tttatatttt caaaattgct gtgaacaaac ttcactctac tttcatattc
    32761 actcaatctc tcttttgctg tgcttatagc atttttatct tggtcaaacc ctataaataa
    32821 tcccttatct gacagttttt ttactatttc tttagaatgt cctgctcctc ccatagtaca
    32881 gtcaacatac acgccatctg gctttatatt taaattctct atacactcat ttaaaagaac
    32941 tgatacatga tgaaattcca tagttacctc ccttaaattc actttttaaa ctattcttca
    33001 ctaatattat ttttattttt ctttgataaa taaacatgtg caattatgcc accaataact
    33061 ccaattagac ttataacttg tgccattcta agtggtccta acattaaact atctgttctt
    33121 aatccctcta taaaaaatct tcctattgaa tataaagtta tataagtaac tataacttga
    33181 ccttcatatt ttttattttt tctaaatagt aataaaacta taaatattcc aaaatcccat
    33241 atagactcat ataaaaaagt tggatgtacc tttactccat caaccattat tccccaaggt
    33301 aaattggtag cacctccatg tgcttctcca ttaataaaat ttccccatct tccaatagct
    33361 tgtgctaaag gcattccaag tataactgta tcagccattt ttaagaagtt aatattttta
    33421 atcttagtgt atatataacc agctaatatt ccaccaatta accctccatg tatagcaagg
    33481 ccaccacctc taaaattaaa aatttgagac atattttgag catagtaact ccaattaaat
    33541 acaacataat agattcttgc acagattagt ccaactggaa ttgctattat agctatattt
    33601 aatacatcat cttctttaat tcccactctt tttgcttctt ttattgctat taaagttcct
    33661 aaaatcattc cacatgccat aagtattcca taccacatga tgtctatgcc aaacagtgta
    33721 aatgctactc tatccataac gtcacctctt tgtattaatt gaaaatattt ccaaagcaat
    33781 atgctataat tacaattaat tgtaattata ctaaattatg ataacacatt aatgttaatt
    33841 ctacaaaaaa tattaaaatt tattctacaa ctccctacac tatattatat ctaattacta
    33901 taaaattttt taaactgaat ctctcatgac taaagttaca agtgtacata gatatttttt
    33961 aatcaaaaag attgatgcat atatacatca acctcttcta tttcttatct atagctctta
    34021 aatttttacc tcaatttata taaatatatt ttataaattt gttttaccaa aataaacatt
    34081 taaaaacata aactaatact taattagtta ttcttattat gattaagttt ctatttaggc
    34141 tctacaatag aaataacaca atactcttct ttaaaatgct cttttaatct ttcttcaact
    34201 tcctcaaaag taacatcttt aataatatct aaataatcca atagatttat atccttaaaa
    34261 acataagata taaaactatt acctataaag tttatagaat caaagcactt aataaatgac
    34321 cctatttttt tctttttagt tctctcaaac tcttcttttg ataatccttc tttttttgat
    34381 ttttctatat aatcaagaat tatttctttt acttttttag gctctttaga atctccagct
    34441 attatgctaa aagcataatc cacttgagac gaaaatccag ctccaaaatt ttcatttatt
    34501 aaaccttgca tatacaaatc ttcataaagc ttacttcctt ttttaaataa catccctaca
    34561 agtatatcag ttacaatctc cttacgtaaa agttctttcc cttttaaacc aacattacta
    34621 tctttaaaac ctatgttgaa cataggcata gatataggga atttttctat aacctctttt
    34681 tcttttacac cttctggttc ttctggataa aatctttcta tactcttaga gagtttgtct
    34741 actttgtaat tgtttgactt tttagttaca tctataactt tttctacatc taagtcacca
    34801 acaacaaata aagccatatt tcctggatta tagaaagtat tataacattt atatagctct
    34861 tccttagtta tcttatatat actatcaaca gtacctgcta tatcaattct agcaggataa
    34921 tttatataca ttgccttcaa gcaattaaag tatacattcc aatcagggtc atcattgtac
    34981 atttttatct cttgtgctat tatacctttt tccttttcaa cattttcatc tgtaaaataa
    35041 ggtgtttgaa catagtcaat tagatgttct aaactctcat aaaaattttc agtagcagaa
    35101 aataagtagg ctgtcattgt aaaattagta aatgcatttg catttactcc taattttgaa
    35161 aatttatcaa atgcatctcc tccatcaggt tgctcaaaca tcttatgctc tagaaaatgt
    35221 gcaatacctt cattcactct aattttctta tcttctccaa ttggtacaaa ttctaagtca
    35281 tttgaaccat aattagtagc caaaatagca tactttttca taaaacccct tttaggcata
    35341 aaatatacat ctaatccatt ttgcaatttc tcataataaa cttcttcttt caatatatca
    35401 ttaactattt tttccataga catctcctcc attaatttct caagaagtat acagtatcaa
    35461 gctctatatt ttttatagct tcaactatat ctttttttgt aaccttttct atactagata
    35521 ttatatcttg tacttcagaa tttgtctttg ccatagattg agagaaaaca aaatcagaca
    35581 taccacctat attatcagtt atagatttca tagaattaac taaagcaagt ttactgtttt
    35641 ctaattcttc atcagatata tcacctgctt ttacactttc tacttgtttt tttatcaatt
    35701 ctacagcttt atcataatct ttagtttcta taccagatga tataaacatg atacttttat
    35761 acttctctat tgaagagaat atatagtaac ataaactttc tttttctctt acattaacaa
    35821 ataatttaga gtgtggacca ccacctaaga cattgctccc aactactaaa gcataatatt
    35881 tatctatgtc tgcataatcc acatttgctc tataacccat aactaactta ccttgagtta
    35941 tttccatttg ttcatctata acttttacct catccacatt ttttataaaa tctgctcttg
    36001 gtatatttat aatttcttct ctttcaaatt ttagattttt acttattata tctacaactt
    36061 tatcttcatc aaaattacct tctacaacta tatctatagg tgatgtttta agtatgtttt
    36121 tataatgttc atataactct cttgaagtta tactatctat ttcatcttca taaccaaatt
    36181 cacttacact atatctctct cctttacaca tagcctcaat acatttatct tgcgcatatt
    36241 cctttttatc atttattatt gattgtatct tttctcttaa attctctttt tcaatattta
    36301 aatattcttc tttaaaccca ccatctacaa caagcgtatt atttattact tcattaaaaa
    36361 attctactac ttcttcaaat atactctcat ctaaatactt ttcacttatg ttaattactt
    36421 taaaactcaa tacttgacgt tctccacgtt ttacagcatc agctcccata gatgaaccat
    36481 ataaatcatc taatttattg gatattgctc tagcactagg atatttagcg cttccacttg
    36541 ctattattga tggtattaga gcattttttg tagtttcttt ttcatccaat agtctctgaa
    36601 catataaact tataagattc gatttaaact tttctgtttt tatcaaagta agattaatat
    36661 tattacctaa atttattatt tttttagtat taaccatatg tacct
//
LOCUS       DAFKMX010000016        36132 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFKMX010000016 DAFKMX010000000
VERSION     DAFKMX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.016"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            32..1144
                     /locus_tag="LZB08_000674"
     CDS             32..1144
                     /locus_tag="LZB08_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBM7314189.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTISISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="LZB08_000675"
     CDS             1398..3422
                     /locus_tag="LZB08_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7314190.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="LZB08_000676"
     CDS             3642..4328
                     /locus_tag="LZB08_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBM7314191.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="LZB08_000677"
     CDS             4423..5478
                     /locus_tag="LZB08_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBM7314192.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="LZB08_000678"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="LZB08_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBM7314193.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="LZB08_000679"
     CDS             7195..8901
                     /locus_tag="LZB08_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBM7314194.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVKFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="LZB08_000680"
     CDS             8923..9996
                     /locus_tag="LZB08_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBM7314195.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="LZB08_000681"
     CDS             10008..10730
                     /locus_tag="LZB08_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBM7314196.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="LZB08_000682"
     CDS             10746..11903
                     /locus_tag="LZB08_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBM7314197.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="LZB08_000683"
     CDS             11906..12745
                     /locus_tag="LZB08_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7314198.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="LZB08_000684"
     CDS             12760..13935
                     /locus_tag="LZB08_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBM7314199.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSE
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="LZB08_000685"
     CDS             13964..14713
                     /locus_tag="LZB08_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7314200.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="LZB08_000686"
     CDS             14735..15643
                     /locus_tag="LZB08_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7314201.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="LZB08_000687"
     CDS             15691..16443
                     /locus_tag="LZB08_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7314202.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="LZB08_000688"
     CDS             16397..17764
                     /locus_tag="LZB08_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBM7314203.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="LZB08_000689"
     CDS             17770..18861
                     /locus_tag="LZB08_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBM7314204.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPGNVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="LZB08_000690"
     CDS             18945..20858
                     /locus_tag="LZB08_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBM7314205.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="LZB08_000691"
     CDS             21057..21764
                     /locus_tag="LZB08_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBM7314206.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="LZB08_000692"
     CDS             22066..24177
                     /locus_tag="LZB08_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBM7314207.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVLINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="LZB08_000693"
     CDS             complement(24375..25340)
                     /locus_tag="LZB08_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBM7314208.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLN
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="LZB08_000694"
     CDS             complement(25625..26581)
                     /locus_tag="LZB08_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBM7314209.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="LZB08_000695"
     CDS             26949..27737
                     /locus_tag="LZB08_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBM7314210.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="LZB08_000696"
     CDS             complement(27860..28330)
                     /locus_tag="LZB08_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBM7314211.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="LZB08_000697"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="LZB08_000697"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBM7314212.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDITLGG"
     gene            29156..30061
                     /locus_tag="LZB08_000698"
     CDS             29156..30061
                     /locus_tag="LZB08_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7314213.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="LZB08_000699"
     CDS             30376..31923
                     /locus_tag="LZB08_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7314214.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVNSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLVDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="LZB08_000700"
     CDS             31926..32324
                     /locus_tag="LZB08_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBM7314215.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="LZB08_000701"
     CDS             32364..34097
                     /locus_tag="LZB08_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBM7314216.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="LZB08_000702"
     CDS             34329..35540
                     /locus_tag="LZB08_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBM7314217.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="LZB08_000703"
     CDS             35733..35993
                     /locus_tag="LZB08_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBM7314218.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT        14465 a         3779 c         6364 g        11524 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aatttcaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaag acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgaaata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccatagtaca aacttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataat aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc tattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atccaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattgcctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ttatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtaaaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa taatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaact ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcgacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga caagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatctatttc cttcattgat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccga
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgagaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca gaaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctattt tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgact tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa accagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatttcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca ggaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagcgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt gaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtaga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatt aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtaattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcagctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta taaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc gcccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat tcaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtgctat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag gcttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagtattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggttagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatatcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactaatcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aacattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaaaaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttaatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaatcttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt gtagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agataatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtaaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataacaat ttttcaagtg
    33781 cagatgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttaggg
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatatagttc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaaccg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag aa
//
LOCUS       DAFKMX010000017        35451 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFKMX010000017 DAFKMX010000000
VERSION     DAFKMX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.017"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..633
                     /locus_tag="LZB08_000704"
     CDS             <1..633
                     /locus_tag="LZB08_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7314219.1"
                     /translation="GTGKSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRV
                     FQDPSLGTCPSMTVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDV
                     QVKFLSGGQRQSLSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITT
                     LMVTHNLKHALHYGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            764..1786
                     /locus_tag="LZB08_000705"
     CDS             764..1786
                     /locus_tag="LZB08_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7314220.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKDKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1950..2507)
                     /locus_tag="LZB08_000706"
     CDS             complement(1950..2507)
                     /locus_tag="LZB08_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBM7314221.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTIAVGNPCRVIKKIEE"
     gene            complement(2513..3589)
                     /locus_tag="LZB08_000707"
     CDS             complement(2513..3589)
                     /locus_tag="LZB08_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBM7314222.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3590..4273)
                     /gene="modB"
                     /locus_tag="LZB08_000708"
     CDS             complement(3590..4273)
                     /gene="modB"
                     /locus_tag="LZB08_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBM7314223.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4422..5234)
                     /gene="modA"
                     /locus_tag="LZB08_000709"
     CDS             complement(4422..5234)
                     /gene="modA"
                     /locus_tag="LZB08_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7314224.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5427..6356
                     /locus_tag="LZB08_000710"
     CDS             5427..6356
                     /locus_tag="LZB08_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314225.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWGDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6517..6966)
                     /locus_tag="LZB08_000711"
     CDS             complement(6517..6966)
                     /locus_tag="LZB08_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314226.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7169..8149)
                     /locus_tag="LZB08_000712"
     CDS             complement(7169..8149)
                     /locus_tag="LZB08_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7314227.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPQGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(8354..9214)
                     /locus_tag="LZB08_000713"
     CDS             complement(8354..9214)
                     /locus_tag="LZB08_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBM7314228.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(9531..10859)
                     /locus_tag="LZB08_000714"
     CDS             complement(9531..10859)
                     /locus_tag="LZB08_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBM7314229.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKTGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(11007..11336)
                     /locus_tag="LZB08_000715"
     CDS             complement(11007..11336)
                     /locus_tag="LZB08_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7314230.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11360..12649)
                     /locus_tag="LZB08_000716"
     CDS             complement(11360..12649)
                     /locus_tag="LZB08_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7314231.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(12665..12991)
                     /locus_tag="LZB08_000717"
     CDS             complement(12665..12991)
                     /locus_tag="LZB08_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314232.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13106..13735)
                     /locus_tag="LZB08_000718"
     CDS             complement(13106..13735)
                     /locus_tag="LZB08_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314233.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(13950..16607)
                     /locus_tag="LZB08_000719"
     CDS             complement(13950..16607)
                     /locus_tag="LZB08_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7314234.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVLYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(16966..17910)
                     /locus_tag="LZB08_000720"
     CDS             complement(16966..17910)
                     /locus_tag="LZB08_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314235.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(17903..18928)
                     /locus_tag="LZB08_000721"
     CDS             complement(17903..18928)
                     /locus_tag="LZB08_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314236.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(19003..20571)
                     /locus_tag="LZB08_000722"
     CDS             complement(19003..20571)
                     /locus_tag="LZB08_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7314237.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(20684..21595)
                     /locus_tag="LZB08_000723"
     CDS             complement(20684..21595)
                     /locus_tag="LZB08_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314238.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(21579..22565)
                     /locus_tag="LZB08_000724"
     CDS             complement(21579..22565)
                     /locus_tag="LZB08_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314239.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            22925..23326
                     /locus_tag="LZB08_000725"
     CDS             22925..23326
                     /locus_tag="LZB08_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7314240.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23329..23727
                     /locus_tag="LZB08_000726"
     CDS             23329..23727
                     /locus_tag="LZB08_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBM7314241.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(24177..24401)
                     /locus_tag="LZB08_000727"
     CDS             complement(24177..24401)
                     /locus_tag="LZB08_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBM7314242.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(24436..25803)
                     /locus_tag="LZB08_000728"
     CDS             complement(24436..25803)
                     /locus_tag="LZB08_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7314243.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(25887..27422)
                     /locus_tag="LZB08_000729"
     CDS             complement(25887..27422)
                     /locus_tag="LZB08_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314244.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(27445..27879)
                     /locus_tag="LZB08_000730"
     CDS             complement(27445..27879)
                     /locus_tag="LZB08_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314245.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(28140..29051)
                     /locus_tag="LZB08_000731"
     CDS             complement(28140..29051)
                     /locus_tag="LZB08_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBM7314246.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            29347..29700
                     /locus_tag="LZB08_000732"
     CDS             29347..29700
                     /locus_tag="LZB08_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBM7314247.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(29919..30860)
                     /locus_tag="LZB08_000733"
     CDS             complement(29919..30860)
                     /locus_tag="LZB08_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBM7314248.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(30961..32196)
                     /locus_tag="LZB08_000734"
     CDS             complement(30961..32196)
                     /locus_tag="LZB08_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBM7314249.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(32240..33094)
                     /locus_tag="LZB08_000735"
     CDS             complement(32240..33094)
                     /locus_tag="LZB08_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314250.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFYEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(33222..34397)
                     /locus_tag="LZB08_000736"
     CDS             complement(33222..34397)
                     /locus_tag="LZB08_000736"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBM7314251.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(34496..35380)
                     /locus_tag="LZB08_000737"
     CDS             complement(34496..35380)
                     /locus_tag="LZB08_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBM7314252.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT        11521 a         6220 c         3787 g        13923 t
ORIGIN      
        1 ggaactggta aatctacttt attaaatata atatccgggc ttataaaaga atcttctggt
       61 caagtattat taaataaaca taacttgtct aatttagctg aacacagaag aactcaaata
      121 gtaagtagag tttttcaaga cccaagtctt ggaacttgcc cttctatgac tgttagagaa
      181 aacttgtctt tagctcttaa caaaggaaaa ctattaaatc taagaaaatg tcttcgttac
      241 aaaagagatt tcttagaaaa tttattggaa ggtgtatctc ttgatttaaa aaaatacctt
      301 gatgttcaag taaaattttt atcaggaggt caaagacaat cattatcact aattatgtcg
      361 tgcttaacaa atccatcagt tttattactt gatgaacata cagcagcact tgacccaaaa
      421 acatcaaatg aagtaattga attgactaat aagattgtaa gggaaaaaaa cataactaca
      481 cttatggtta ctcacaactt aaaacacgct cttcattatg gtgatagatt gataatgctt
      541 cataagggtg aagttgtatt agatgtaaaa ggtaaagaaa aagaacaatt aacagttgaa
      601 gaaatcttag aaaaatttga atatgcagtt taaaataata tatctattgt attaaactcg
      661 gctttaaaat aaagttaaaa ttacaataat tatggttact ttgtaataat tcatatgaat
      721 atatgaacat atataataat aaatttttga gggggatata aaaatgatta ataaaaaaag
      781 attggcaagt ttgattttag ctggtgctct tagtatatct atgcttacag ggtgttcaca
      841 aggaggagac tctggtaatt caaaacaaga aagtaattct aaagataaag aagttaaaaa
      901 aattggtata actcaactgg ttgaacatcc agcattagat gccactagga caggttttgt
      961 aaaagcatta gaaaagaacg gatttaaaga tggagaaaat attgatatag acttccaaaa
     1021 tgctcaaaat gacatgccta ctacacaaag tattgcgagt aaatttgcat ctgataagaa
     1081 ggacttaata tttgctatat ctactccatc agctcaggca gcatttaatg ctactaaaga
     1141 tatacctata cttattactg ctgtgagtga ccctgttgct gctggattag ttaaaactct
     1201 tgaaaagcca ggcacaaatg tatctggaac atcagatttt gtttctgtag acaaaggttt
     1261 agaacttctt aaaatatttg ctcctaaggc aaaaactata ggagttatgt acaatactag
     1321 tgaagtaaat tccaaagttc aagttgatgc tttaaaagaa tatgcatcta aaaatggatt
     1381 taaagttgtt gaaaaaggta taactacttc aaatgaagtt aaccaaggta tttctagttt
     1441 agttggcaaa atagacgtat tatatgtccc tacagataac ttagtggctt cttctatgcc
     1501 tatagtctct aaaatagcta ctgagaataa aatacctgtt atagcagctg aatctggtcc
     1561 agttgaaaaa ggtgctcttg cttgtcaagg tatcaactac gaaaaactag gttacaaaac
     1621 aggtgaaatg gcagttaaaa ttttaaatgg cgaatcagta tctgatatgc ctgtggctac
     1681 atcagatgat acagatatca ttgttaatga agatatatta aaagctttag gtatggaaaa
     1741 accttctaat gaaaatattt cttatgtaaa gactaaacaa gaatagaaag atattttcta
     1801 tatacgatta atatgcctgt ttctataaat taatttatag aaacaggcat atatttttat
     1861 tttaatgtat tatctattta aagattatat gtaaatgctc ttaatttgtt tgtataaatt
     1921 ttacttgtat aaactttata caatcatatt tattcttcta ttttctttat tactctacat
     1981 ggatttccaa cagctatagt atttggtggt atatcttttg tgactacact tcctgcacct
     2041 attactacat tatcacctat agtaattcct ggtgttatga ttactccccc acctatccat
     2101 acattatcac ctatttttac aggagaacca tactctatac ctgaatttct aagttgtgca
     2161 tcaattggat gataggctgt atagatttga acgtttggag ctaacattac attgtcacct
     2221 atttcaattt tacatacatc taaaaaaata caatcataat ttgcaaagaa attttcgcct
     2281 acatgtatgt tatatccata atcacaccta atgttttgtt ctacatttat ttgcttgcca
     2341 actgaaccaa atagttgtct taaaatatct tctctttttt catattcatc ctctaaagta
     2401 ttattaaaca atctagttaa ttttttacaa tactctcttt cttttactaa caattcatca
     2461 tttgcatagt atcctttccc agacaacatt ttttcttttt cagtcatata gtttactcct
     2521 ttttgttata attgtatcta aagcaaaata aatattcctg tggaaaatct aaatatattc
     2581 tgtcaccttt cttaaatctc attttacttt tttccaattc tatttgaata ggtacacatt
     2641 cgttagataa atcagttttt ctaacatata tagtatatgt aaaaggattt tctacaatgt
     2701 taatcaattc gaaacataac ttttcattta aatcttcatt agaatccaag accttaatat
     2761 gatgctccct gattcccaca tattctatat tatcacctat ttctctattt aatgtcaact
     2821 cgcatcccca gtctgtagca taaatagtat ttttatccag tctatttaat ttggatatat
     2881 ttttacaccc tgttatctta gcttctataa ggtactttgg agattcaaag agctcttttt
     2941 taggtctctt tggaagacct tctcctttgt tgtacactat aatatcatca caaactcggt
     3001 aagcttcttc aatatcatga gtaacaaata gtatatctcc cttgtaatct tttaacatat
     3061 tcataagttc tttttccata ttgcttctta aatgatggtc caatgctgag aaaggttcat
     3121 ctaaaagtaa tatatctgga gatgttgcca atgctctagc tagtgctacc ctttgttgtt
     3181 gaccaccaga taattgccaa gggtatctcc cctcaaaccc atttaattta agtatatcta
     3241 agtaccttgc tactatttcc tttttttcac tcttacttag ttttaaaagc ccaagttcta
     3301 tattttgact tacagtcata tgtggaaaca aagcatagtt ttgaaataaa aatcctacct
     3361 ttctatcttg tgttgataaa tttatcttct tttcagaatc aaatagaact ctatcattta
     3421 atacaatttt acctcttgtt ggtttctcaa gccctgctat acattttaat gtcatacttt
     3481 ttccagaacc agattctccc aaaaacccca gagtaccttt ttcttgttta atttctacct
     3541 ttagtttaaa agaggataaa tctttttcaa tatccacgta taagctcaac tatccacacc
     3601 tctttcctat aattttctgc tgaaattccg accaataatt tagtaataaa atcattgcta
     3661 tagatattgc tacaataatc ataacccaca tcatagcttt attcatatct ccgccttcaa
     3721 cagcaaaaaa tatagctatt ggcatagttt gagtttttcc aggtatattt ccagctatca
     3781 taagtgtcgc accaaattcg ccaagagctc tggcaaacga cagcaccaat ccaccaatta
     3841 ttccaggcca tgctaatgga actgcaatct taaaaaatac tttccattca ttaagaccta
     3901 atgttctagc agctgacagc atattattat caatctgttc aaaagctgac cttgaagttc
     3961 tatacatcat aggaaaagac accactactg ctgaaattac agttgctgtc caactaaata
     4021 ttaaagtcct atcgaaattt aataaaaact ttcctataaa tccattcttc ccacatagta
     4081 ataataaaaa gaaacctaca actgttggtg gcaaaattag tggaagtgta aatagtccat
     4141 ctataagacc cttccactta cctctatagt tagccataat atatgacaca aatattccta
     4201 ttacaaaagt tattattgtt gacaaaatag atgtcttaag tgatatccat aagggtgacc
     4261 aatctgttcc catctttccc tccttaattt taaatggaaa tagtgaaact aataactcta
     4321 ttaatttcac tatttccatt ttattataat acacaattat tttcaatttt catttattta
     4381 tatataattt atacttaatt acacataatt tatttctaag tttattctac ttttttatat
     4441 ccaaattctt caaaaatctt ttgtcctgac tcacttaata aaaattcttc aaatttctta
     4501 gcagcatcta cgtttttact agctttaatt actgaaactg gatatgtaat aggcgaatga
     4561 gttttttcgt ctgttttttc aactactttt atcttatcat tattgacagt atcactgaag
     4621 taaacgaatc caacatcagc atttcctgat tgcacccatg ctaaaacttc ttttacatct
     4681 tttgcaaata ctagcttatc ttttaactta tcttttaaat ttaaattagt taacacctca
     4741 tctgcatact taccagctgg aacactttca gcttcaccaa cagcaatttt cttcacttta
     4801 tctgtagtca aatctttcat acctgataca ctactgtcag cagaagaaat aagaactaaa
     4861 tcatttttta ctaaatcttt catagtatca gaaactaata acttctcttc atccaaagct
     4921 ttcatttgtt tttgacctgc tgaaataaat aaatcacaag gtgctccttg ttctatttgt
     4981 tgttgtaatg agcctgatgc tccatagtta actgtaagtt ttacatttga atcaactttt
     5041 ttatattctt cttcaatctt tgccattgct tcttttaaac ttgcagcagc cgatatattt
     5101 aactctactg aatcactact tttagttgtt ttttcttgtt ttccatcatc ttttttgtct
     5161 tcattactat tgcacccaac tgtccccaaa gttaaacatg caactaatcc taatattccc
     5221 aatatttttt tcataacaat ctcccttttt gtaaatttga tacacatttt aacatgtttt
     5281 attatgtttt atcatgtttt gttatatata gtttaattat atatacatga taataaattt
     5341 tcaagtaatt tatttacact acttttaaat attttgctac attttccttt ttttaatgtt
     5401 aaaataacct tatatggagg tggattatgg actcattatc atctttgaca cctgtcgagg
     5461 tagctgaatt attaaagata acaaaaaata ctgtatatga attaatcaaa agaggtgaac
     5521 ttccttctta taaagtaggg aaaaaaataa gagttgatac taaagatgtt gaagaatata
     5581 tcaacagtca aaaaactggt aaagtaaagg atacacattt caacaataaa aatatagata
     5641 taaataataa tttcaaaatt attatttctg gtcaagatgt aatattagac attttagcta
     5701 ggtctgttga aaaaaaattg gatggaataa gtacatttcg ctcttatata ggtagttata
     5761 atgctttgta tgagttatat aatggcagag tttctatagc atcatcccat ctatgggatt
     5821 tcgaaacgga tgaatataat agcacttttg ttaaaaagct attgccagga ataccatgtg
     5881 tacttataaa tctagcttat agaatgcaag gtttctatgt tgctaaagga aatcctaaag
     5941 agataacaac ctggggggat ttaacaaaac caagtataac cattgtaaat agggaaaaag
     6001 gttctggaac tagggtttta ctagatggta aacttagact tctaaacttt aatggtaaac
     6061 atataaaagg ttatgataat gaagaattat cacatcttgg agttgcaagt actgtttctc
     6121 gtggaatagc agatgttggt ttgggaaatg aaaaagctgc tttacaagta aataatatcg
     6181 actttattcc tcttcacaaa gagcgctatg atttagttat taaaaaagaa gacttacaaa
     6241 atccagttta ccaaactata attaacataa taaactctcc tgagtttaaa gctgaacttc
     6301 aaggtcttgg cggatatgat ttaactgata ctggaaaaac tatagcaaaa gtttaagatt
     6361 aaaatcgcaa tctattttat aagatttata atttttcaca aaatttataa cttaaacacg
     6421 aaataaattg cgattttagt gtctcaaaaa atttatgtct ttcattttat catccaatac
     6481 aaaatttaat ataattaatt gcgcaatcat actatattaa tatatattta cataatagta
     6541 tttttcttta atgtagatta acatatgata tgttttaaac atcaataccc ttgctttctt
     6601 atatatatgt ggcaacacat atgagtttaa taaattatat acaatgttgc tttcttcttg
     6661 tattttttca accaacaagt tatttataaa attacctttc tcattttcaa taatctcact
     6721 ccaatttatc ttaactttta atatatatct tcccactagg tatacacact ctacaataaa
     6781 taataaaaat acaatgcagt cagtacctgt tgtcttatat aaattacccg atactaatgg
     6841 ttttgcgaag aaattataaa aattcaaagt atcacccgta taatcaaaaa gtattaatag
     6901 cactcctaga actctcaata ctttaatatt caacatgttt ttatctttat tatttttgtt
     6961 tttcatatat actacctctc ttttctttag atttgattgt aatctaatac taactctttt
     7021 atttgaggta taaatgaatt taattggtag ttattttcat ttttactaga aattaattca
     7081 cattactaaa caaaacttat atttaaacat aaaaatagcc tatctatttt ttaataaata
     7141 caaaaataaa taagctacta atattcaatt aacttttatc acttaaaaca ttactatcta
     7201 taaatttaaa tattttattg aagtatactg tactatcatg ttcaaatcca tcaacatgac
     7261 caataccttc tataagccat aggtcttttt tatcatgttt aactaaatca tacattttct
     7321 tgctattttg ataattacaa acagtatctt ttgttccatg tataaacagt atgggtacat
     7381 tagatttctc aactgactca tatggtttta catcactgta ccaaaatcca gatttaaact
     7441 tattatataa atctccacaa gtcaccacat aattgattaa tatatctggt attcgttttt
     7501 ctagtactcc cattctaatt gcatctttca tttcagaata aggagaatcc agtatataaa
     7561 aatcgacctt atcatctttt gaatttatct ctgaatgcat agcagctgtc cctgccccca
     7621 tggaaaatcc atgcactcct aatctccctt gaggaaattt attttttaca aatttaacta
     7681 gactatctaa atcatacctt tcataaagac caaatgtgta attatctcca ccactattac
     7741 cagtatttct ttgattataa actaatacat tataaccttt atctaaatat ctatatgcta
     7801 cctttaacat ttcatagtaa ctacttccaa ttccatgaac aagtatcata gtatctcttg
     7861 tctttatatt tgaagttata aaaagacttt caagcttgta attattagaa ctttgtatca
     7921 ttaattcaga tgtcttgtag ttttttaatt tctccagtgg tttatctttt ctctcagaaa
     7981 atatttttac tatatcttcg ttttttatta actgctctaa accaactgaa ctattataca
     8041 ctaatttgcc aaatatataa gatacaatta ataggattaa tagaaacaca attgatataa
     8101 ttataaattt atttcttaaa ctaatacaat ttttaaaact atttttcata tttatttacc
     8161 atacaatttt cctatattat attttctaag taatagcata attgattata ctattcttat
     8221 aatttctatt atacctactt atttattaaa aatacaaatt atattttatt aatttttact
     8281 acttgtaaat tattatctta tacaatgaat ttgtattata taataagtat ttttaagatt
     8341 ctgttacata atattattta aatattcttc tatatttctc ttcttcttct tcagtaaaca
     8401 cattaaatac aacttttaaa tttttttcag gattttcttt tagccatagt ctaactctac
     8461 ttacagcaag attagctgcc tctttttttg gataaccaaa tacacctgta gaaattccac
     8521 aaaaaacaat attttttatt tcatcaattt cttttattgt atttaaacat gatttataac
     8581 aatgaagcag ttgcttttct tgctctttac ttggctgacc accagataca ataggtccaa
     8641 cagtatgtac tacaaatttt gcaggtaaac aataacctct agttatctta gcatctccag
     8701 tatattctaa atgcccttgt ttttttatta ttttgtcaca atcttctcta agtctaggac
     8761 ctgcgcatga atgtatctca ttatccacgc atagatgtaa tggttgtaaa caccctaata
     8821 atttgttatt tgcagcattt actattgcat ctgctcttaa attcgttata ttacctctcc
     8881 atatggcaat cccttcctct acttcttcta tatcatttac atctactaac tctctttctt
     8941 cacattcaat caacaataaa ttatgtaaca tattaagtat ctctggagca atttcttttg
     9001 gtggatatac attcaaaagt cctctaagta tatctctagg cttaatttca tcatttgata
     9061 aattgtatat tttagaagga acttcttttg aaaaatatgc aattaaagtg tttatatttt
     9121 ttcttctttg ttcgtcagtt agaggcttga tagttttatc aaaatcctca aataaattta
     9181 catcatcagc ataatctctc cacttcatag ccatctctcc attcttgtat tttagactta
     9241 aaaataatta tgtatttatg tacatattta tttatttcta taaacaccta cataaatcct
     9301 gttaaaagtg taaatttttt attatgcaac ttctctggtt atttctattg attgatagta
     9361 aattattgta cctaaattta atccaactaa caagtaacta aacctacttt cctaactgca
     9421 aaatacacta tgtttgccac ttgtatctca tttgtagata aataaagtcc ctacaaatac
     9481 attaactagg ctatgtacta aactgtagga actttaaaaa tatattattc ttaattaagt
     9541 tttggccaat aatttttatt tgcttctatt aaatcatcta gtaattcttt tgcaactgtt
     9601 gcactaggaa ctgtcttaga tagcataagt gcttgccaca atttattata agacttctca
     9661 atccaagcat caactactaa tttttcaact gctaattgtt gttctattaa acctttttgg
     9721 aatgttggta ttttccctat tgataatggt tcatacccgt cttttccaac tatacatgga
     9781 atttcaacca tagcatcatc ttgtatattt tctatagctc cattattttg aactatcaat
     9841 aacatacgtt cctgtgtatt aaaagctaga gctgtagcta aatccactat aaactctgca
     9901 tgtattcctg tcttaagccc actgccctta gaatgccctt gcttttctat caatgcacat
     9961 gaaccaaaaa cttctttttc tcttccatca ataacttcat tggcacgagt ataatttaca
    10021 tcagaatgat ttacaacata atctggaaat agatagtact ttaagtaact acttggtatt
    10081 gtagttaaat ctgtttttac tatttcttta gtaaataaat tagtttcaaa ccaactatct
    10141 ttcttgtcat ttttcattcc ttcttcacca tttataaaac catattcttt tatatgttct
    10201 actaattttg gtaaaagttc atttcctttt ttatctttaa ttgaagtata ccaaccaaag
    10261 tgatttaatc caaagtatct tatatccata tgtttacgag atttgagtcc tgcaatcctt
    10321 gctatattat gttccatacc tattggcata tcacaaatat ttattaccct agaattaggt
    10381 ctcaaccttc taacagcttc cgctattatt gatgctggat tagaataatt taacatccaa
    10441 caatttggag aatacttttc catataatct atgttttcaa gaacaccttc tatagacctc
    10501 attccataag ctattccacc tggtccacaa gtttcttgac caactacccc atgttttaga
    10561 ggtatctttt catctagttc tctcatttca agttttccaa ctcttatatg agctaaacaa
    10621 aaatctacat ctgtataagc ctctttaggg catgttgttg ctaaatactc tatattaggg
    10681 tccttttcct ttattagtat ttcacaagct ttagctacct tattttgtct ttcttcatca
    10741 ttatcatata atttgattgc tcttaatggt aacctatcta agttatctag caacattaat
    10801 atgatttctg gggtatatgt acttcctcct ccagctatta ctactgaaaa tttttccata
    10861 ataatatctc cttttctatt taaaaattta ctgtatttat aaataaattt ataaatacta
    10921 gtactttaaa gtaaaaggga gttcatttat taaatctatt gatttaataa actatagcca
    10981 aacttcaagc actatttaca agaaatttaa ttcttattaa ttcttgggtc catcatctca
    11041 aatatatcta caagttcaga tactaaagaa tgagcaaata atgcatttga ataatggtct
    11101 tcagagtgca caactaaaaa atcagtccca ctatcttcac tattcatagc atttgatagt
    11161 aattctaaat gatgctttga tgctgtttgt attttctctg aggctgaatt taaaaggtgt
    11221 tttgctttat tgtagtcacc tttttttatt tccttaagag acatcattac atcactttta
    11281 gcatctcctg cttctgatat tatactaaat actttttctt ccatttcagg taccatatcc
    11341 ttctccttct aatgacttgt taaaaatcaa agctatcatc atctaatgtt gtagctaatt
    11401 ctgcattttt atttaattct tccttctcca ttatcttaaa gaatggataa tatatcaagg
    11461 tcataagtat tatacttgca aatactaata cgattgcctt ccaatctaat gtcatcaaaa
    11521 atgctgcaac ccctggaggt aaaaagcctg gtggttctgc aataggtaat cctactaatc
    11581 ctacttttaa tgccataaaa ttaagtgctc caaccaatac aggtccaaat acaaatggta
    11641 caaacatata tgggttcata actattggtg ttccaaatat tactggctca tttattccaa
    11701 atagtgatgg taaaatcaca acttttccca tactattaaa actcttacac ttacttcttg
    11761 acataaggta cactaatccc aaagttatcc ctgaaccagt tacttgcaag aaaccatagt
    11821 aaaaacctat tgtaaacaca tattttgggt ctcctcctgc tgctatcagt gttgcatttt
    11881 ctgccatata tgtagctgct attggcatcc atatacttga agttattgct gctccatgaa
    11941 gcccaaagaa ccataataat tgagttataa atagtattat agttactgca aaaacattat
    12001 ctacactgct tatagctggt gctaagaaat tcattataat agctggtagt ggttgacctg
    12061 agacattttt agttattgct gctattgctg taaataacat tatacatact atcattggag
    12121 ttaagtttct aaaactttta tttactacac taggtacttc ttttggaaat ctaattacga
    12181 gatttttatc attcataaac ttagtaatat caactactaa aagtgctact aatatactta
    12241 caaataaacc ttgtgcatct aaataacttg tatctaatac accatctact acttgtccac
    12301 atacaacaag ttgtgcaaca actgctgtca tacctatttc tacatgatta agattatatt
    12361 ttttagctag tgcctgagct ataaaaaatg ctgaatatat agataataaa ctcatagtaa
    12421 atttattagg gataagtaat gtatccatat tttcagataa taatgttttt gcacttcctt
    12481 caggtaacat attgtttaat gccataaata acatacttaa agaaccaaca attattattg
    12541 gggtaatgct tatcatacct tcttttatag cttgaagatg cctttgtttt tcaatctttt
    12601 gtgctattgg aagaagaaat cttcttaaat tattttgaag tgcttccatt tttattcccc
    12661 ccaattatct atttttatat aattcgattg ccgacttcat aacagaagga gcatccatac
    12721 gaccataatg cttagtatct attaaaccaa ctgctatttt gtaattctta caagcttcaa
    12781 ctacatcatc ttccttaaac ctaatttgtg gtgctacaag tactacatct gtatcattag
    12841 cttcaatgta tgattttaaa ttttcaaatg atgttgcctc tatatttata gttttagttt
    12901 ttaacttttc atgctcacta caaaaacttt tcatcttttc cacaagtaca cctgttgaag
    12961 ctccatttgc acatactaat aaaatattca taattaccct cctacaatat ctaacctctt
    13021 ttgttttcta attttttatc agccaattct tactctaatt cctatcagtt tttactttaa
    13081 ttcttattaa tatttactct aattcttagt actgccaatg attaaacttg aaagccgtat
    13141 ctataacttc ttcttgctta tatttttcta atatttttaa gtatctttct ttataatcat
    13201 tatctacttc tttttctggt attactatac tagcttcatt taaaaagtgg aatgaatccc
    13261 ttcccatttc taatcctcta tatgtatgct tgtcaagtgc cacgtccata acttctggaa
    13321 ctttacccat tgcaaatcct tttataatta tattttttaa taaatcactt gatctatctt
    13381 tctcacatgc acaaagatat cttattgcat gtatgaaaaa taaaggtctg tctccatctg
    13441 catatgaata ttcctttctt acttgattta aattatttat gattattaca gctgatgggt
    13501 cccccattcc tatatcttca actgatattg taagtatcct tctccatagc ttatcttcta
    13561 attctggtga tgatatatat agttcataag caaattcaca agcttcttcc accatatttc
    13621 ttcttatagc tttttgaaat gacgatatta tttcgtctgc actaaatccg tttcttgtag
    13681 tcattcttgc ccatgggtca tttgcctttt tatctcttaa tattttatct tgcatattat
    13741 ttactccttt ttatttaaat tttaattatt tatattgctc atttaataat ataagcaagt
    13801 attatgccag tttttttaga cactttaaaa cactgttatt tcaatcattt ttatcatcaa
    13861 aacttagata ctttgtctaa gtatatttat atgtgtctaa tattaatatt attttagact
    13921 aaaaaaaatt accaaataag tttttatact cattggtaat ctttttcgat tatttctgca
    13981 tattctaata ttaataatat ttgatagcac tctttttcat taatttctat attatatttt
    14041 ttatctaatt gagatactat atcttttatt ttgcttaggc tcttgtgata atcaagtgtt
    14101 ttttctttat caagcttttc aatttctcta gtatttccaa tcaacccatc taccatgaaa
    14161 gctaaatgca tcataagtcc aattacttta tcgtatttct tttctatatt aaatactaag
    14221 aacaattcat cagacacata ttttatatct ttaaatatcc tattataatt agagcttttt
    14281 aaggatgtct ttaaaccttc aactacatca tcataaccta tacttatctt tataatattt
    14341 tttaatttat taatattttc atccttatct atatcttttt tgctaatata gtcaattcct
    14401 ctaatattaa ttctttgttc attgattact gcaacaatat tactactttc tttaagtttc
    14461 gtaatattta aacttaaatc tttttctgtt ttatatagga cagatataat atctattcca
    14521 tctaagtacc tactcatctt ttcttctaat atttttcttg ttttattatc tacaccttct
    14581 gaaacatgtg atgctataat aattgtttta gacagatttt ctttattttg aatttcatta
    14641 gttccaaaat agtactttct cgtgttagtg atagagttgt atacgtcatt taaaggtaat
    14701 cccatattgg ccttctgaac tgcttctaac acagttaatg tcgtcaccat aggaattact
    14761 cttactccac actttttttc tttctttata atttcatcaa aagagtttaa tgaacccata
    14821 tctgtaaata gtaatacatc tttattgtct atattattaa ttaaattttt aaaatctaca
    14881 attacttcat catagctttt atctaacttc atatcgaaag aaatagcact gcactttcca
    14941 ataatatcac caactgcttt taccatacta cttgctgtag tttctccatg catagcaact
    15001 ataatattaa tgttattcac tattttattt tgttcatctt gtttagcttg tactatgaat
    15061 aatgtaatat atgctacagt ttctatagat aaaaataaat caaattcatt ttcaattatt
    15121 ccaactattt ttagtgctgt tttaaattca cttctgttat ttcttctaat ttgattcaaa
    15181 tctatgttgt ttgtagagct acttttatcc aatacgcttt ttatataaat tgccattgac
    15241 ttaaatattg attcacttat atctatttct tcatcttctt taataatttg tcttattgat
    15301 tcacacatgc ttttaaactt aacattatta aatagagaag catctgctat attcatttct
    15361 acagatttat taagattagt atttactgca ttaataatat ttatatcatt gtagttaata
    15421 acttcttctt ctcctaatga cgtaaataca aaatattcta ttccatgtgt tataacttca
    15481 tctgctttaa tagacttctc tctataatca tcactcactt catttataga tttatcaaaa
    15541 tattttttct ctatataaac tggttcttta ttatctcttt tatatcttag gtatccattt
    15601 gcaacagcta attttatatc attttgaagt tgacctacat tatttttaca atcataaagt
    15661 aatagtgatt taattatttc tttatgtact tttattgaaa tagaaatact ttttgattct
    15721 tccttgaaaa aatttttgac taattccatt ctttcaatca ttgtcctatc tctcagtgat
    15781 ggtatttcta ttttcattgg tattcttcta ttgaatgtat ctaatagtgt tgatgtcgga
    15841 ttttctgtag tagcacatat tattaatacc ttagactctc tttctttcgt tgcatctcct
    15901 agtcttttaa atgttccctt atctataaaa gtaaacaaca tttcttgacc ttctgctggt
    15961 agtctatgaa tttcatctaa aaatagtatc cctccatttg caatttccaa aagacctttt
    16021 ctggaagatt ctgcacctgt ataagctcct tgttctaccc caaaaatatg tcccataagt
    16081 aattgtgcat tattagcata atcagcacaa ttaaatgtta caaaaggagc tttatttttt
    16141 ctaattccac tatctataga ataatcgtac atatatctag caatcatact ctttcctact
    16201 cctgtttctc catagataat agtatgaagt ccatttgtag gatagagcat tgcgaccttg
    16261 catttttcta ttataggtct taaactatgt tctttaccta ttagtgtttc aaaactatct
    16321 ttttttattt tgttttctac ttttgttcct ttttttatgc caagcaactc actttgtgta
    16381 tcaagcaatt cattcttgtt aaggttaaga tttgctggga tataatacac aggtcttcct
    16441 tttatcttcg taaccatacc ctcattgaat aattggttta aatatgtact tatattgttt
    16501 cttgcaattc ctgtcttttc tgataattgg ttagctgtaa tccctttttc attttcccta
    16561 tatgatgatt ctaaaacttt cattacttta tcttttttat atatcattac aagctcccct
    16621 acattataat attttattaa ctaaaacccc tatacacact ttatttttta ataaactctt
    16681 attctttaac taaggttata atttatcaaa ttttccttac tttaaattat agaaaattat
    16741 tgtagaaaaa ataagattat tgctcatttt ttctacatta ctatttttaa ttttatagta
    16801 ctttcttaac aacatacttt acttgttttt aattttatta tatttgaatt tattatattt
    16861 gaaaagtaga aataaaaaac cctattttgg agtagtttta aaatttctcc aaaaataggg
    16921 ttttagtttg tatttcaaat aaatacctgt tcaagtattt ttatactaaa caacttttaa
    16981 attttgttga tatttattta tttctgattt agaagaatat acaaaatgtc catctgaaac
    17041 ctcaatccaa cttaatgact tttcatttga acaatcatat ccattagaat tatactctat
    17101 tctatttctt gacttagcat atctagggtc aggaacagga attgcagaaa gtagtgattt
    17161 tgtatatgga tgaataggat tattatacaa ttcatttgaa tctgccaatt ctactaattt
    17221 tccattatac ataactccta tacggtcact aatatgtctc accatagata aatcatgagc
    17281 tataaataaa caagttaaat tcttttcttg ttgcaatttt ttaaataaat ttactatttg
    17341 tgcttgtata gacacatcca aagaagctat aggctcatca gctattataa attctgggtc
    17401 tactgaaaga gctcgcgcta tgccaatacg ctgtctttgt ccacctgaaa aatcagatgg
    17461 atacttattt gcataacttc tatcaagtcc tactagcttt agcatctcat atactttatt
    17521 cattctatct gttttatttt tacagatacc ttgaattttt agtggctctg aaattatatc
    17581 tgcaattgtc atacgtggat ttaatgatga agtagaatct tgaaaaataa tttgcatact
    17641 tttgtttaca tcttttttta taaatttgta cgttttttta tctgatatgc aatttccttt
    17701 gtaaattact tctccaccag tagattcatg tagtcttatt atagttttac cagttgtaga
    17761 ttttcctgaa cccgactctc ctaccaatcc aaatatctca cccttatata tatcaaaact
    17821 tatatcatca actgctttta cagtagtact tttgtccaaa tgaaaatatt gctttaagtt
    17881 ttttatttcc agtatttttt ctctattcat tagaaatcac ccttccacaa tttactctaa
    17941 ctggtacatc tacttcaggt gcatctgggt gcaataacca agttgctgca ctatgtgtat
    18001 cattaatttt aaacattggt ggctcttttt cataatctat cttaagtgca tttttattcc
    18061 ttattgcaaa agcatctcct tttggtgggt tcaataaatt tggaggcatt ccaggtatat
    18121 tatataaata gtcatcctgc gaatctaaag taggtaatga acccaacaac ccccaagtat
    18181 atggatgtct agggtcataa aatatatcct ctacagttcc tatttctaca atctttcctg
    18241 catacataac tgctattcta tcagctatag ttgcaactac tcctaaatca tgagttataa
    18301 atattattga caaacctatt ttatgttgta aatcctttat taaatcaatt atttgagctt
    18361 gtatagttac atctaaagca gttgtaggtt catcagcaat caatacatct ggattacagg
    18421 caagtgctat agctattact atacgttgtc tcattccacc tgaaaaatga tgtggaaatt
    18481 gtttaaatct tttctcagga ttatcaattc caaccaattc aataagctct atggctctct
    18541 tttttgcttc acttttactt ataccttgat gaatgattac agcttctgca atttgctttc
    18601 ctattgatat agttggattg agagatgtca tagggtcttg aaatatcatt gatatattct
    18661 ttcctcttat atcttccatc tctttttcag acttcttaac caaatcactt gatttaagta
    18721 aaacctctcc atttttaata tatccattgt atgggagtat cctcatcaaa ctcttacaca
    18781 gtacagattt cccacaacca gattctccaa ctatagccaa tgtctcacct ttttttagat
    18841 taaaagatac atttcttaca gcctgaactt ctccttcttc tactttaaaa cttacagata
    18901 gattattaac ctccaataaa tgttccaaaa accatcaacc cctatgctat tcaaacctta
    18961 gggatatatt tttatcccta aggtttctaa ttaatttcag tattattcaa ttgtccagtc
    19021 agctatgttc cagaatatac ctacaccatg atgacctaaa actgtatcag gtgttaatcc
    19081 ttttatgttt ggttttccaa catatattgc atctatataa gcaataaatg tataaggcat
    19141 atccttagtc atttctactt ggaattgttt atataacttt aatttttcat ctttatcttc
    19201 tgtttctctt gctttttgaa gtattttatc tatagtagga ttagagtaag cactgtaatt
    19261 tgcaccttta tctgttccaa atactttata tgtatggtca tctgggtcaa atggacttcc
    19321 ccaacctatt aaatgagcat cttgattagc ccaatctgtt tcagttacaa caacagcttt
    19381 agcatcaaca ccaatttctt ttaactgttg tgcacatatt ttagccatat caactctaac
    19441 ttggtcactc tcacctgctg ttatctcgaa agctaactta gtaccttctt tttcatagat
    19501 tccatcagaa cctaatttcc aacctagctt ttcaatttct tgtttagctt tttctggatt
    19561 atattcaaac ttttctatat caggattatt atatggtccc atttgtaaag gtgaatatgc
    19621 tggtacacca tgtcctaata atacactgtc aactattgct tttctatcta ttgcatagct
    19681 taaagcattt ggtaatcctt tagctttttt atctttaaag aatttagaat taaaattgta
    19741 taatatacct ctatagtcgg ctgttttcat tatatttact ttaaaattct tttcgtcttt
    19801 ttcaaagtta gacatatctt taggagttat ttgagctagg tctaattctc ctgattttaa
    19861 ttgcattgcc ttagccttat catctggaac aatcttaaag actactttat ctaatcctgg
    19921 ttcttttaca aaataatcac tattcttaac aagtgttata ctttgacctt tgtcccattt
    19981 ttcaagttta aatggtccag tacctattgg tttttgatta aattcatcag tagctatatc
    20041 cttaccttcc aaagcatgtt ttggtaatac tcctactgtt aaataatcaa gcattgctgt
    20101 atttggagct tttaaagtaa tttttatagt attatcatta accacatcaa tttttgttat
    20161 atcttcataa tttgatgcta tctcagaagc attatctgga ttcattatag tttctattgt
    20221 aaacttaaca tcatttgctg taaatttctc tccatcatgc cacttaacat catctcttaa
    20281 attaaaagta tatgtatttg ttgcctcatc aaatttccag tcctttgcta gacaaggaac
    20341 caccttatta ttttcatcat gagctgttaa tccattaaat attaacgaat ttatttctcc
    20401 atgttcatat agtgcaggat ttatacttgt atagtcatta cttccataaa caagtacctt
    20461 tccatcttta ggcgtatctg ccttcttatc tttgtctcct ccactagaac aacctgccat
    20521 tagactaagt atcattacta aacttaaaac ttttaacttc tttaatttca tgatgctccc
    20581 ccttttgctt tttataaaat atttttcaaa gctttgaccc catttattta aatagatatt
    20641 tcaaatacct cagtatttgc ttatttagta ctcaaatgct gatttataaa ttactagatt
    20701 ttttattatt attctttctt atataattac ctatatttgt tatacacacc aatgttgtaa
    20761 ccaaaaatat acccggaatc agtataatcc accatctatt tgaaagtaat gcttcctctg
    20821 acaaagataa catacttccc caagaaacaa tttctattgg taaaccaatt cctaaaaagc
    20881 taagtgtcga ttcagtacca atagctgcgc ctatgtttgt tacaaccatg aacattattg
    20941 atgccacaaa atttggaagt aaatgttgct ttaatacata gaagaatcct cctcccatgc
    21001 tcttagctgc tagtatgtat tcgctatttc ttatttgtct aacttctgtt ctcactatct
    21061 tagatatatt catccaactt gttatcccta ttactatgga catagatact ggatttgaat
    21121 ttccaagtat agcttgtaca aatattataa ttaatattga aggtatacta agtatgattt
    21181 ctgtaaacct catcattgca tcatctacga gttcagatgc tgaaccactt atactaccat
    21241 aaacaattcc tataacagtt gatattacag tggaaaatag tcctataaat aaagatattt
    21301 ttcctccata ccatatcata gaatatatat ctctacccat tgagtctgtt ccaaataaaa
    21361 aagttttatt tggagctaaa ttagaacttg ctaaatccat gtatgttggt tcatgtgtca
    21421 ttataaaaga tgagaataca cttccaatga ctatgattga taaaataata attgatatat
    21481 atggtaattc tttaattttt ttagataagg ttttctttgt ttctatatgt tcatcttctg
    21541 ctactaaaat ataattttct cctacaattt caaaatcact atattgtggc ttcattagta
    21601 tcacctctat catatttcat tctagggtct attttattgt ttataatttg agctaacata
    21661 ttcccaaata ctactaaagc ccctgttatt aagcataaaa ctaataacat attataatca
    21721 tgatactttg cactttcaaa acaaagtgtt cctaacccag gataagaaaa aactttttca
    21781 actacataag ttcctcctag tatatgaggt atcgaaatgg ccattatact aatatatgat
    21841 ggcattatat ttctcaggca atgcttaaaa acaatagttc tattatttaa accttttgct
    21901 ttacataaaa gcacataatc ttctcttatt tcctctagta atttatttct tatcatatat
    21961 gtgtaatacc atagatggct taatataagt actgtaagag gtaagattag atgtgatatc
    22021 ctacttaata tactggactc ttctcccatt gaataagcac cactacttgg aagtatactt
    22081 aagtttatac taaatataag tatgagtact aaggcaaccc aaaatgatgg aatacagttg
    22141 gttattgttc ctaatttgca tatgattcta tcaatgagct tatcttcatg taatgtacaa
    22201 aatattccta acaataaggc tagcacaaag gtaagtatat atcctgaacc acctaatatt
    22261 atagtgttta tatagacatc atttataaca gaagtaacat tttgtttata tttaaatgaa
    22321 attccaaatt ccccttttga tgcatttgaa atccatttta tatattgact gtatattggt
    22381 tcatccaaac caagtttttt catagcattt tctttttctt gtgtactcat tctttctact
    22441 ccatccccat agtaagagat taatgggtct cctggagcaa gtcttgccat gtaaaaaaca
    22501 ataaaagata ataaaaacat tactaaaata aactgcaaca actttttcaa tatatccttt
    22561 aacaaacccc aacccccctt tgttgcaata aaaaaaacca cataaaagca aacgtaaagt
    22621 aacgttaaaa ccttcgtggt tccattatca ttaaacaaaa ttaaattttc tatatttatc
    22681 tatgagttaa ttttagcatc atttattaac tgtgtaaaca aactttttaa aaattgttaa
    22741 aatttcatca aattatttta tttataccta aaataaaatt tttatattct attcttcatt
    22801 attttctata aaattataaa attttaggtt gactatataa gaaaagtata ttatattagt
    22861 ttgtatgcaa accatttaag aggtgatttt attgaaaagt aattatgact gttttagaat
    22921 agccatgctc ttaaaagaac tttattctaa aactatgtac acagtagaag aaaattttaa
    22981 agaaaatgga ttaacacacc aacagattat agttataaaa ctgatagctc ataatcaaga
    23041 acttacaata tcacaactat gtgatgaaat gtctttagct aaaggtacgg tttcaggcat
    23101 aatcagcaga ttagaacaga ttggatatat agaaaaattc aagaaatcta atgataaaag
    23161 aaatacatac gttaagttta caacaacagg atttgaattt gccacaaatt ttaaaattaa
    23221 aatgcaagaa agttttgatg atatttttaa aaattgtgat gaaaacgaat tgagtgactt
    23281 agtaaaaaat cttagaaata tattggcaaa agtaaaggag agataattat gaattataaa
    23341 ttaatacttg caatagtatt tataacatca gctttaattt tctacactat aggtgtattt
    23401 ggagaaagaa aggcaaaaat tttaaaaaag aaacatgtaa ttatattttg gcttggtttt
    23461 atatttgata ctttaggaac atttacaatg agtaacatag ccaacagcca tacttttgaa
    23521 gtaaaatctg ctttatcaca aaaccttcat agtattacag gtcttcttgc aattgtgctt
    23581 atgctttttc atgcaagttg ggctactttt gtcctataca aagatgatga ggaaaagaaa
    23641 aaattctttc ataagtttag tatagttgta tggactatat ggttagttcc atattttata
    23701 ggtatgttta ttggtatggc tggatagtaa aataaaaagt gtgtaagaaa tactgtaggt
    23761 aaatttgtat aagacaaatt tatctacatc cttctttcac acttttacat atttatttat
    23821 aatactccat tatttctttc atgttcctta atacctatat agaatattta attgtttcta
    23881 tagtatttta ttattttctc acactttttc atatatatat tgttttacta tttcttcata
    23941 tgtatatatg aaatatttca ttatttctat aaatgtttta ttatctttct catacttttt
    24001 catatatata ttgcttcact attttttctt atttcttcat atatatatga aatatttcat
    24061 tatttctata atgttttatt atctttatca tactttttca tatatattgc ttcactattt
    24121 tttttacttc tttattaaat acttatgtat aacatacatt taataaacgt taactattaa
    24181 acacctaata cttgagttac tcctggaact tcactcacca aaacattttc aataatagct
    24241 ttgattgtca tagtcgctcc tggacaccca ctacatgccc cttgtagtct aactaaaacc
    24301 acaccatttt catttacatc tataagctct acatctccac catctctttg taatactggt
    24361 tttatctttt cctcaagcac tttttcaact ttttctctca tagctattcc ccccagaaat
    24421 attttattac caaaattata ccaatggttt aaatttttgt ccatctaatg atttatcaaa
    24481 ttcaattttt gcttcatcaa taattttagt atccataaat acatcataag cactacaagc
    24541 cagtacttta gctgaattta tcatagcaga aaatcctata tttgaacctg ctgatgaaca
    24601 tgactgccat gtatgacttg caattccaac aggccaagct gccattgcaa attgagccac
    24661 tggtattaca taacttacat ctccaacatc agtagaacca gctagtcctt tatctttgtc
    24721 accgtcaact atttccttgt gtatatagct ttctacaata gatttatcat tttgtaatga
    24781 tgttgctaca cctaatcttt cttctctagt aagagtatca cacaattttt ttgcaaattc
    24841 ttcttcttca ggagtatttt tagggcaacc aacaaaaacc atattgtcat aaactaaatc
    24901 agtcaaacat tggtttggta tatagtcata tattccatct gtaaccttat actccatttc
    24961 agtttctgtc atcattgctg ctccttgagc tacttttatt aatctatcaa gaacgtgttc
    25021 tgcatccttt gcttttttac ctctaataaa gtaccaactt tcagcaaatc caggaactac
    25081 attaggtctt ccaccaccat tagttataac atagtgcatt cttatagagt caataacatg
    25141 ttctctcaaa taattagctc caacattcat aatttcaaca gcatcaagtg cgcttctacc
    25201 attatgagga gcttgagctg catgggctgt tatcccttta aatttaaatt ttacagataa
    25261 gtttgccaga cttccacctc tccaaggtgc atttatatca aaaggatgcc atgtaaatgc
    25321 acaatctaca tcatcaaaac agccatttat acacataact gtctttcctc caccttcttc
    25381 ttcagcagga caaccaaaat attttattgt tccattaaat ttattttctt ctattaattt
    25441 ttttatagaa acaactgctc ctacacctgc tacacccaac aggttatgcc cacatccatg
    25501 tccagctttt ccttctacca atggctttct ttctgcactc acacattgtg atagctctgg
    25561 taaagcatca tattctccaa gtatgccaat cacaggttta ccctctccaa aacttgctat
    25621 aaatccagta ggtaaatttt ctacttcttc aattttaaat ccttttgact ttaggtattc
    25681 tttttgaatg ctacttgcat acttttcttc aaaggctaat tctggatttt cccatatttt
    25741 cttagataaa tcaattaatt cttctcttat ttcttcaaca ttatttatga tatactcttt
    25801 cataattttc ctccaaatat atgtattttt gatatgcttt acagattata ttatatacta
    25861 acattctatt tattcacaat tttttgttaa ttaattttat tcatatgctc ttctatggag
    25921 tatatcttat cttcactttg cctcttcata aatttaatgt actctatctt ccataaagta
    25981 gtttcagttt tctcatttaa cataaattct aatatattaa tttctctcat tctaatatct
    26041 tcataaaata ctacactacc caatattgcc aattctaatt cacttaatga tgcaaacaac
    26101 attaaatact cttctccaaa taaacattca gattcaagaa tatcgataaa gtctttttta
    26161 gcattgatat tttcctttat tattcttatt ttttcaatta tagatgtact atctaatatc
    26221 tcattgaata tctctctaaa cgattcatca tccaatttac tttcatcatc aacaatataa
    26281 tcttctaagt gttccttttt atttttactc acagttacca tacttttaaa agtgttactt
    26341 tttattgagc ttagcacaga ttcaataaat atcttttcat attttttaat atatcttatt
    26401 acatcttcat cttctatgct tagtcttttt attaatactt caattgcttc cataagtagt
    26461 aaagttattt ttttatttat tctaatttct ctgttaattt cttcaaaatc attataatcc
    26521 aaattactat ctacttcagt gttaaaacta tcatctactt caatcaattc aattatatta
    26581 ctttcttcag agtctataac tttatcttca gtaatattat tgtacatatt tttaaattgt
    26641 ttttctaaaa attcatactc atattttgaa atttctaaat tagtcacttc atttcctaaa
    26701 atagtagaaa aaatacaatt atttataata agttcaaata aatttacaag tataacatca
    26761 caatctattt tataagtcac cccatagtca taaaataatt tttctctatc ttttttctta
    26821 aataggttac aaaatctatt ttctatatct atactctcaa tataattctt cacataataa
    26881 atacccttaa catccatatc atctagtgcc aatggataat ctatacttac tggaacgtcc
    26941 tgagcttcaa attttacaat ataactttta aagaaacttg ataactccat caaggtatca
    27001 ttatatgcta ttgaattagt atccaatttt ctactcatca ttagtttata aaaatctttt
    27061 agcctaacta actcttcctc taatatttca atgcctccaa ggtacacctt tttaacattc
    27121 ttagcagtca actccacaac tcttttttct atatctccaa gcttatttat atatgcatca
    27181 atagcatacc atattgaaat caacagtttt tcggcaactt ctgtttttac tgatgtgctt
    27241 tgaccatttg tatatttttt aatcaaatcc ataagtatta gagatatttc ttgttgtata
    27301 ttatatactt ctctttcatc tatcacttta caatttagac attccttaag tagagatatg
    27361 gaatattggt tctttattaa attattttcg tttatattaa tattacttaa attatttaac
    27421 atacaaaatc tctcctatca tatattaaac ttctccaaat ttaaatttct ttgcaaattc
    27481 gtctgcttct cctcttacat tgtccatatt gcctgaaaat ttttcataaa attcacccag
    27541 tatttctata atctcatcat cacatatctt gtcatcagtt tcatttttta aataataaaa
    27601 aatttcttgc atatcattaa tagcctctaa ataattgtcc acatttgtat attgtgatgt
    27661 atatatattt tctattaact gttttgtaac ttttatatct aattctattc ttccataccc
    27721 ctttaaagta ttatctctcg aatttattat atcttttaca tcagattcag aaagagctag
    27781 accataatct ccagattcta tattagtttc aagcagttca tttctttcta tttcctttaa
    27841 attctgattt atagaactca aaataatagg tgatatcatt tttaaattct ccttttttat
    27901 tttttccatt gacttttttt gttttttatg ctatgcttaa attagtatat attattttgc
    27961 caaccactta taatagtagt gttaaaagtg gtttttgtca actcaaaatt actatctcaa
    28021 ttaaactttt caattttctc aacaaaaaaa ataccatcat ttagatatat aattttctat
    28081 aacaactttt aattcagtag ttatttacta aaatctaaac aatgatatat ttattaattt
    28141 tatttatctt ttttggctct acttctactt ttctttacat ttgagcaatt tctacaagaa
    28201 ggtgaacagt attgagattt agaatttgta ggaatataaa ttttaccaca gtacttgcat
    28261 ttatttaaag aagaaggttc acttgtaatt gcaaaggcat agatagtctc aatagctgtt
    28321 ttcaaagaat caaactccca tgcaataatt ggtttatcta attggcttat agaaaatcct
    28381 atcttttgag gattaaaccc tccagacata atagtaacac tttcagttaa tagattagat
    28441 gtatttttat atacacaaat ttgattaaaa tgcttacaca tatctttagc aaaacttaca
    28501 atccaatcta gttgttcagt gtagaacttt gaaaatacta aatctaaaat caatggtctc
    28561 tttccaaaaa actttggaga ttcttctctc tttgatacat ctatacccct tctatacttt
    28621 ttaaaataca catcttcatc acttgcaaat ggtataaaca tgtttacata ttcatctgta
    28681 ctcaatatat tctcttttgt aatataattc ttttcaatca tcagtactct ttcttctcca
    28741 actatatctc tattatatac acttgaatat attaaaccca ataatccata cttttttaca
    28801 aattcaagta cttctttctt tagtttcatt tcttttgtct cttttgatat atcttgtaat
    28861 gcctcttgtc ctatgcgaat tatatctact attatatcat ctgctacatc aaatgggtta
    28921 tacatggtaa acacagcatc ttctgttgga actatatata attcatcttc ttcagtaatt
    28981 ttatattcat aatcactata cctaatccaa tcacttgcaa agttccctat taattctatt
    29041 tttttattca taataatcat tcacaccctt caatgtaata tataatttca tattactatt
    29101 acacttttag tttattccat attttctaca ctgtcaacta ttctttttat atttttagca
    29161 tattaaaatt tataatagcc tttataatgg atttctacag ctaaatttct tgtttattga
    29221 tgactaattc ataattaaat ttataaaaac tatttacata taaaattaat ctctttataa
    29281 ttgatatgta atatattttt acacttacat attacatatc aattataaag agaggtatct
    29341 aaaatcatgg atagaaaacg tgaattaaaa caattataca aagaaatgaa atttgacaca
    29401 ggtgtattca taataaaaaa tgatattact aaaaagattt accttggaaa aagtaatgac
    29461 ataaaaagca aatttaactc tctcaaattt cagcttggtg ctggctcatg tatgataaaa
    29521 gaattaaata atgaatggaa aaaatatggt gaaaaagcat ttacatttga agttttagaa
    29581 cttataaaac atgatgataa caaaacagag aaagactatt tagaagaact agatatttta
    29641 gagatggttt ggttagaaaa attaaaggaa gataatactt atgaaattta ttccatataa
    29701 aaaataatat aatataagaa aaggagatag tatatcaaaa tagacattta gttcataaga
    29761 tactttttta tataaaataa agtatctttt cataatttta acaatgaaaa agaggcttat
    29821 ctccatttga tagcctcttc ccatatattt tataccatta taaaatgaac aatctactaa
    29881 ttttcaatgt gtatattcat tttactaata acactccatt acttctttac agcatccaaa
    29941 cattgctcta taatactctt atcacttata cctattgctg ttactttact agctccactt
    30001 aatatactct tatcactttt atttgaaact aaaacaacag gagcattttt tgcaagtggt
    30061 gatgcaacta aagcataaac taaatcgtct ccacttgtta tatagaactc ttttgctcca
    30121 ttatagaact tttcaataac atttttatta gtttgatatc tatcaacacc accaattcta
    30181 gttgctctat atctgctcac tatgtaatca cttaatgtag actctcctcc aatagcatat
    30241 actggaacag gagaaactcc aaaccatggg tctaagtctg tgtcttcttc tgctgaaggt
    30301 atatcttttg tcaatattat aggagctcca tctctataag caacagatgc aacacttact
    30361 gcatcagctt catccttaaa tccatttact aaaaacatct tatcactgtt tttaattttt
    30421 tctatttcac ctgcaatttt ataacttgta tctactctat ctacaccatc aattcttata
    30481 acttgcattc ttctcataag taacattttt tctacatctt tacttataga attttctcca
    30541 cctattacat atatcttagt aactctattc agtctttgaa gcgttgcttc tggtatatca
    30601 tccttattag ttaataaaat agctgcatta tttactcctg caagaccact tgcagcaatc
    30661 ccatctacca ttgtagaatc tgcattgata agaactgctg ttgtaaaatc ttgcttatct
    30721 gaaataagcc cagctgtttc atatttatct actccttgaa tattttcaat cttatctagt
    30781 gcgtttattg atgttggaaa actaacactt aacagtaatg ctgaaatacc taacgccaat
    30841 acctttttac taatcctcat aaattacctc cctaaaacaa atatatttag tattagtata
    30901 atataaaatc atttaaaaat agtgtaaaaa tagttacact tttttataat ttaaataatt
    30961 ttagactaaa cacaattcat ccattaaaat aacttcattt attctatatg ctaagccatc
    31021 ccacgttttt tttgacatat ctacagatgt tttcttattt gagttattca ataaagattg
    31081 tatcttagaa tctattgcat tatacaaatt attttcaaaa tcatataact cttcatcttt
    31141 tggaatacct tctttctcca taaaaggtag agaaacatat tctatctttc cagaattatt
    31201 aatctcacta ccaatccact ctttaacacc aagaatatca gtagtgatta catctgtgcc
    31261 acaagataaa gcttctaaaa ctacaactgg taatccctca taaaatgatg gtaacacaaa
    31321 tatttgtgac ctgttgaata gctcagctaa atctctctgt tctaactttc ctaggaaatt
    31381 cattttaaat ggagacttac ttgccagttt aactatctca ttatatgatt cgatatcgct
    31441 tccagtacct gcaaaattga cttctattaa atctttacta tattttaatt tagatatagc
    31501 ttttataaat ggaatcagtc cctttgattt acatatctta cctgcaaata ctatttttat
    31561 tttatcatcc tttattttat aatttttatt ataaaatata tcatcattat aaccactacc
    31621 aattacaact actttacttt cacttactcc aaaagtcttt ataatatcat atttctgatt
    31681 ttcatgaaga gcaaatatta aatctaattt ccttatattt gctattatgt attctttctc
    31741 taaatcaatg gtatttaact gtcttaaaca agttccatga caaatagaca taacctttat
    31801 atcttttacc atttccctaa caaatgcagt taaaaggtac aaatgatgac atattataag
    31861 ttctggttta aaatcattca tagccttatc tatatttact ttaaattgat atttcaatct
    31921 atttatcatg tctatattta aatctttata tcttgtactt tcatatggca tagaatcact
    31981 catacctaca acaggaaaat ttaactcccc acaattatac tttactggat aaaaacttac
    32041 ttcacttgga aaacaattta catcatcatt tacatctata cctgcaatta cagcttgctt
    32101 atgaccgatt ttttcgaacc cttttatcat accagacaaa tatataccac tacctgtact
    32161 atttggtttt tgtgttatta tatgaagaat tttcactttt cacttgcctc ctttctaaaa
    32221 ttaaatagtt ttatacagtc taatcattat atatatctaa atataaacaa gatgaatcat
    32281 cttttacttt aaatcttggc actttaaccg ctttatagtc attatcttca aaatccctta
    32341 ctttattgtg tatatatcct ataccaaatt tttctgctat acgtatcaat tcttcttctt
    32401 ttattagatt atatctatca catgcacttg taaaaccatc actcgttagc atcaattgaa
    32461 aatctttttt tatttcaatg tatccatata tagaattttc cacagcttcc tcatcaaaat
    32521 ccaatatcca atagccctct tttgtatttt tcttcaatct attgcttact attgtatcca
    32581 taacactagt tctaatctca tcaaatgcca tctcttccaa gttagaaagt ttactcattt
    32641 cataaaatac agtattatca aatttacaaa tactcctatc ttttataact ttggaatctc
    32701 catttttaat aaacaaagca caatctccta ataaaaaata ctctacttta ttttcatgaa
    32761 attttacaac aactatagaa ctagatggta aatctaattt ttcaacctta aacccattta
    32821 atcttagttc atactctttt tttacttcta ttattccagt attaataata ctctttaatg
    32881 attcttcttt atttatattt ttatataaat acctattcca ccactgaaca taccaccttg
    32941 cgtcactttc agttgacact aaattttttc cattaagtcc tgtagcacca tctaaaaccc
    33001 atgcgccaaa tggatttata ccgactacat cttcattaaa tttagctgtt ccttgattac
    33061 ataataggct atagtttctt atatttaaag ccatatcaac ctccaaattg aatatctaaa
    33121 aataaagttc tatttaaaat tatacagcaa ataaaaaaat ctacaacaaa acttgttgta
    33181 gatttttact taatctatat tatctttata ctttcagtat tttaatgatg gaataatcta
    33241 ttcttagtaa atgccatgac tatgttatac ttattacaag ttaatataac attatcatca
    33301 cgaatagaac cacctggttg tgctataaaa cttaccccac ttctttttgc acgttctata
    33361 ttatctccaa atgggaaaaa tgcatctgaa ccaagtgcta ctcctgttaa agttttaagc
    33421 catgctcttt ttccttctcc agttagaggc tctggttttt cttcaaaaaa gttttgccat
    33481 atgccatcag caagcacatc catataatca tcagaaagat atacatcaat agtgttatct
    33541 ctatctggac gacctatatc ctttttaaat tttaagttta atactttagg atgttgacga
    33601 agataccaag tatcagcttt gttacctgca agtctagtac aatgaatacg tgattgttga
    33661 cctgcaccta cccctattac ttgtccatct ttagcataac aaacagaatt tgattgtgta
    33721 tattttaatg taataagagc tataattaaa tcacgttttg cactatctgt aaaaatttta
    33781 ttatcagttg gaatatcttt taataggtct tcatttatta atatttcatt tctaccttgt
    33841 tcaaaagtaa ttccataaac atcctttgtt tcaataggtt caggagtata attttcatct
    33901 atttttacaa tattatagtt gcctttacgc tttcctttta atatttcaag ggcttcatct
    33961 gtatatcctg gagctattat accatcagaa acctctcttg ctataatagt tgcagtctcc
    34021 ttatcacata catctgaaag tgctataaaa tcaccataag aagacattct atctgctcct
    34081 cttgccattg cataagcaca agctaatgga gaaagctcta aatcatcaac aaaatatatt
    34141 tgctttaaag tatctgataa aggtacccct actgctgctc ctgctggact tacatgttta
    34201 aatgaagttg ctgctggaag acctgtagct tcttttaatt ccttcacaag ttgccaacta
    34261 ttaaatgcat ctaaaaaatt tatgtatcca ggttttccat ttaatacctc tataggaagt
    34321 tctccattct tcatataaat ctttgatggt ttttgatttg gattacagcc atactttaat
    34381 tctagttctc ttgccatata ctcatccctc tctataataa attaatcata agtttgatta
    34441 atcctatatt aattaaatta ttcgtactaa taaaattata tggcatattt atccattaat
    34501 caactatatt ttttaaaact cctattcctt cgactttaca ttcaactata tcgccatgct
    34561 ttaaatatac ttgaggattc atccccatag caacccctgc tggagttcca gttgaaatta
    34621 tatctcctgg ctttagagtc attcctcttg ataaatcatt tattatagta ggtatatcaa
    34681 atataaagta ctctgtatta gaatcttgtc ttacttctcc atttactaca ctacttatat
    34741 ctaattttaa aggatgttca aattcttctt tatgtactat acaaggtccc atagatgtat
    34801 gtgtgtctaa gctttttcct ctaaaccatt gaacatgttt atcttgaagt gctctttcac
    34861 ttatatcatt tactattgta tatccaaata tatattcata agcatcttct tttgatatat
    34921 ttataccttc tttacctatg attactgcaa gttctgattc atagtccaac ttctctacca
    34981 aatctccatg taagcttatc ttatcatcca ctccaattat tttgtctgct ctcttagaaa
    35041 aataaattgg ataatctggc ataactacgt tttttataac tccatctggg atttcattga
    35101 catggtcttt atagtttaga cctaaacata taatatcttt tttaggtctt acaattgggg
    35161 aacataattt aacttcttct aacttataac actttgcgtt ttcaaatgaa ttgtttttta
    35221 ttttttctaa atctcctgta gatacgtttt caataaaatc attcatatca gtatataatt
    35281 tacttaatcc taaactatta atttcatata tactatttgt ttctgaatta aatacaccta
    35341 ttttttcttc attgctacta caaaaagtca caaatttcat ttttattcct ccattcaata
    35401 agttgattaa tattatttaa tttttattag tttatataaa ttgtaattca t
//
LOCUS       DAFKMX010000018        33049 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFKMX010000018 DAFKMX010000000
VERSION     DAFKMX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.018"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            194..628
                     /locus_tag="LZB08_000738"
     CDS             194..628
                     /locus_tag="LZB08_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314253.1"
                     /translation="MYIENIEELKELIELFGVVEAKSENVVAFGEVESLDSCTEENAE
                     KALDILNKHKQENKKRYRDFFELKEWINGDEERKQIFADIAICERCGKIVANSEMLTS
                     FPILAKYDENRSVFEQLETVAICPDCLMFITRVYESLFDFEG"
     gene            726..887
                     /locus_tag="LZB08_000739"
     CDS             726..887
                     /locus_tag="LZB08_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314254.1"
                     /translation="MKKISEMSILEKADFIRNRQLELKKKYQNKAKGELFNMACRELE
                     EKEKCEGVF"
     gene            892..1236
                     /locus_tag="LZB08_000740"
     CDS             892..1236
                     /locus_tag="LZB08_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314255.1"
                     /translation="MLGYKLVRTEELLKLRKDLEIKEEELKRNLEELSEQHLSKASLY
                     MNLGMTITENEFYNSIIEKLFDKDFRKAISIFNKTKSIRIKNKKATEILLRIDREEKI
                     KEFPCRNKRRKC"
     gene            1239..1634
                     /locus_tag="LZB08_000741"
     CDS             1239..1634
                     /locus_tag="LZB08_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBM7314256.1"
                     /translation="MNNVCLVGRLTKDPEFKYIPKSGTPVATFTIAIDRDYVNKEGQK
                     ETDFIPVEVMGKSAEYCASYITKGRLVALKGEIRVDSYTDKKTEEKRIFTKVHTKMVT
                     SLESSKNNKENKEETLNLQAIYDDPDIPF"
     gene            1692..1838
                     /locus_tag="LZB08_000742"
     CDS             1692..1838
                     /locus_tag="LZB08_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314257.1"
                     /translation="MTRKGKVLKICGWCGKTFLSDNDGKIAYCSKKCKKKREKNLETE
                     GAKK"
     gene            1948..2217
                     /locus_tag="LZB08_000743"
     CDS             1948..2217
                     /locus_tag="LZB08_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314258.1"
                     /translation="MENIFWWSFMDLEEAEKVVTEESIKDLENAINEIENEAEEVKIL
                     IIKKNGDEKYINFKHDGYMKDVIKDRIVQCFEEIKIMHETILEEI"
     gene            2236..2496
                     /locus_tag="LZB08_000744"
     CDS             2236..2496
                     /locus_tag="LZB08_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314259.1"
                     /translation="MNFKMIANVPESWTVQKQFEKILEEILELKEAIALDDNKKILEE
                     GLDVFQAILTLFKIIGIHNISEGLKEHNKKLRRRKWKLEKID"
     gene            2744..3205
                     /locus_tag="LZB08_000745"
     CDS             2744..3205
                     /locus_tag="LZB08_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314260.1"
                     /translation="MKIKKEYIDKVENILKMLGDEYRKLRVLKKEIAILKHKENYREI
                     NYEELGFKVQKSVKSIDDMVITIEDVIYNKETEIEIIERKLEFYNIYLKELSELEQKM
                     IELIYFYNWDEKLPITKIAYELNYDRSSLYNKKAIAINKIALMIYGDEALE"
     gene            3307..3774
                     /locus_tag="LZB08_000746"
     CDS             3307..3774
                     /locus_tag="LZB08_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314261.1"
                     /translation="MVELKKEYIDEVIKLLDEVRELRVRKEVLNDEIEFLKKNEKLSS
                     INFNELGFPVRSGSYSIDDMIINSQEKIYIKETEIEIIDSRIEMINIYTKRLSEEEQE
                     IISLRHFDSKINSYGEISELLMISKTVVQRKYTDALRKIVLMKYGEKAKKDRE"
     gene            3944..4522
                     /locus_tag="LZB08_000747"
     CDS             3944..4522
                     /locus_tag="LZB08_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7314262.1"
                     /translation="MRRTQEVLDIQEYLRYKSYRNYVIFMLGITTGYRAGDLVKLKVR
                     DVREALKRQEFTIMEGKKANSKNIREKNRKPRTVEIRPRIAQILKKYIKDKHDYEYMF
                     PSRKRKYPHIGVEAVSKALKEAGEYFGLYDITAHSMRKTYAYKIYIDSEKDIVAVKEL
                     LGHSSIEETKMYLGLDKELYHHYSESLDDFVR"
     gene            4719..4934
                     /locus_tag="LZB08_000748"
     CDS             4719..4934
                     /locus_tag="LZB08_000748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314263.1"
                     /translation="MKKIELDDKEIKLIISALDLKVSRNLGKAYKNFPYAFDEKEEYK
                     RDVATMELLIDKFLSKLNEVECEGVLI"
     gene            4934..5227
                     /locus_tag="LZB08_000749"
     CDS             4934..5227
                     /locus_tag="LZB08_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBM7314264.1"
                     /translation="MAREFSRSFYNSKAWKECRQSIIKKYLGLCAECGKLGEEVHHIK
                     YLTPANIHDVEITLGEENLILLCKDCHSKKHKSKKDITRTGLKFNEKGELISI"
     gene            5324..5713
                     /locus_tag="LZB08_000750"
     CDS             5324..5713
                     /locus_tag="LZB08_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314265.1"
                     /translation="MGGGLFKIIYEQLEKEKKIKQEVSRLKKNYKDLEKEKVKILDGL
                     INEAAFLKISLEGTREILTKEGLTEIFKQGKQEFERERLQVKIYLNFMKLYSSVMKQL
                     IDIIPSDKKQEEEDKLIEFMKKGRLQK"
     gene            5710..7359
                     /locus_tag="LZB08_000751"
     CDS             5710..7359
                     /locus_tag="LZB08_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBM7314266.1"
                     /translation="MTYIEEYYQKILSGEIIACSRIKQVYKKLVQDLYNPKDNWVFDE
                     ELANRPIEFIETFCKQAQGKLGEPLRLELFQKAKHQAVFGFVDKETGFRKYQEVLDIR
                     GRKNGKTTELAADELFMLIADNEGSPEVYNIATKYEQAQKGFKECYKMVQQSKILSKH
                     IKKRKSDLYFHANYGFLQALASNSNGLDGLNSHMVTIDELAAIKNRDIYDLMKQSMGA
                     RNQPLLNCITTNGFVREGIFDAQYEYACNVLDGKIKDDRFIAFIYELDDKDEWDREEC
                     WIKANPGLGTIKKLDFLRDCVNKAKTDPSFKPTVMVKDFNMKENSATAWLRWDELNNE
                     TKFNVDEMGFRYGIGCFDLAETTDLASAKVLLRKRHDDNIYTLSMYWVPSERLEQKVD
                     EDKIPYDLWEKQGLLRVCEGNKINPYDILLWFREIREEYDIYISWIGYDPWHVDSSLL
                     LAYENEFGKDAMIKVRQGVYTLSAPMKELRADLKANKVIYNNNPIDKWCLSNIEIKTD
                     INGNIQPIKGMDRRRRIDGGVTLIIGYVVLKEKMSEYENMI"
     gene            7375..8529
                     /locus_tag="LZB08_000752"
     CDS             7375..8529
                     /locus_tag="LZB08_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBM7314267.1"
                     /translation="MNIFKSKKKNKEAPERIVMELISDLGNGFYSWHGNLYRSDIVRS
                     IIRPKAKAVGKMAAKHIRSNETEFKTNPEPYIKFLLENPNPFMSGQILQEKMVTQLEL
                     NSNAFAVIIKDDDNIPTQIYPLNALNFEAIYEDRVLFLKFLLRNGKVVTYPYSNIIHL
                     RKDFNENDLFGTPPTKVLKALMEVVNTTDQGVVKAIKNSNTIKWLLKFKTSLRPDDIK
                     KEVKEFEKNYLQIDSEAGGAAATDSKYDAEQVKAESYVPNAAQMDKTTQRLYSFFNTN
                     EKIIQSKYTEDEWNAYYESEIEPVGLQLSKEYTQKLFTRKERSFGNEIIFEASNLQYA
                     SMSTKLNLVQMVDRGSLAPNEWRKIMNLSPIEGGDKPVRRLDTAIVEGGD"
     gene            8535..9272
                     /locus_tag="LZB08_000753"
     CDS             8535..9272
                     /locus_tag="LZB08_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBM7314268.1"
                     /translation="MASDNLKEFLKVKNSTETNADLFFYGDIVCDEWDAWTEEDQYPL
                     AIKDFLAQEQGKDLNIYINSGGGSVFAGMAIYNMLKRHEGFKTVYVDGIAASIASVIA
                     LAGDRVVIPQNAYFMIHKPWIGLCGSYNSDKLIKAAEDLDRIEEGILNVYQDNLKEEI
                     DIEEIKEKLSEETWFTGKEASNYFNFEVDEKKEVAACVSDYFDKYNKVPHALKNKIDK
                     TSNKKENNNKKRVQLKLNLLKLGGLND"
     gene            9265..10533
                     /locus_tag="LZB08_000754"
     CDS             9265..10533
                     /locus_tag="LZB08_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBM7314269.1"
                     /translation="MTREEYFKKRQEMIDEAQKLLDDEVGEEGTGEEKTEEAEKIANK
                     IKALDEEYERNVKARANLRALQDDFKVNPTIFNLTNNKGKIEGIEDTTVKDKQEQYKN
                     AWAKDMLGKPLSSEEQEIFNSINAEYRAEVQTSENNTILIPKTVASGIWKEIGDMYPL
                     FGDASPTFVAGDLTIIAEEDGGDDAAWYDEETEVKEDGYKLKEITLRGCELAKDITVS
                     WKLKKMSIDEFVPYITSLLAEKMGAALAKAIVDGKGKPGESDSFKPQPLGIKTALAKE
                     TSKAQIIEYVDKIAYTDITKLMAVLKKWSNGACIYANSTTIWTQLAEILDTTGKPIFI
                     PDAVNSDGVGRMFGRAVKMDDSMADGEILAGNIAKGYAININENVTLYTDEHVKSRKT
                     DYLTYSLVDGNVISNKAFGMIVKKTSAVAK"
     gene            10543..10830
                     /locus_tag="LZB08_000755"
     CDS             10543..10830
                     /locus_tag="LZB08_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBM7314270.1"
                     /translation="MIVSLEEIKEYLRLEADYKEDDNLLLSFLKAAEEDLENRTGKVF
                     NETNKSELVSLYVKMYVAEQYEKRGATESNSEKVRFVLESIISQISICSRY"
     gene            10832..11170
                     /locus_tag="LZB08_000756"
     CDS             10832..11170
                     /locus_tag="LZB08_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBM7314271.1"
                     /translation="MNVGKLTQRIEIQIYGEIENDIGEITKGWSTYKKLWANKSLLRN
                     SNNYVLDKENIEYSYRFKIRYRTDITEAMRIVCNDVVYDIKHVNNIKELNKYETNIDC
                     ILYKEGVYNE"
     gene            11163..11579
                     /locus_tag="LZB08_000757"
     CDS             11163..11579
                     /locus_tag="LZB08_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBM7314272.1"
                     /translation="MSSSTDFNTNGLDDYTNKLFKRIAKEYPKKAEKLMNISLGKCKG
                     EAIARTPKSDKKPKKYKRAKHMKDNWKTKVQSKNGNFVGVLKNDSPHAHLIENGWVTK
                     NGGYVEGTHMLQQTMEHQRVKIDKRIEKMVDETFNL"
     gene            11591..12007
                     /locus_tag="LZB08_000758"
     CDS             11591..12007
                     /locus_tag="LZB08_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314273.1"
                     /translation="MLKIVSVKKAIVEKLKSLNIKTVANEIRSGFKKPAFFIQIIPIE
                     MASDPSFSNSTLLVNIHYFSEEKTELENLKMIDKLNILFQDCILEIDGGELTIEEKSV
                     EIYENVLQYKFNLQVVEIIEEDESKYELMEELEMNI"
     gene            12025..13092
                     /locus_tag="LZB08_000759"
     CDS             12025..13092
                     /locus_tag="LZB08_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBM7314274.1"
                     /translation="MGLPSAIIEFQRRSRTVKFRSQKGIVALILKDSTATKKSYSIDF
                     LTDINEAEFTKENYDYIRLAFLGKPSKVIIEVINDSADVERTLDDVLKALKANKFNYL
                     AIPWISEEADKTKIVNWIKTARREKEIYKAVLPNIADANEKAIINFATTGIKVGEKSY
                     TTAEYTARIAGILAGIPLSESCTYFVLDEVTEIEPIENPDETINKGELILINNNGIRI
                     ARGVNSLVTLSKEDTEDLKKIKIVEAIDMIQDDILQTWNENYVGKVTNKYDNKVLFLS
                     AINNYFKELQRDEVLDNSQEAYAQIDIEAHKKYLKEAGIDYSEMTEQQIKEANTGSYV
                     FIEGNITVTDAMEDLKFKIYM"
     gene            13106..13555
                     /locus_tag="LZB08_000760"
     CDS             13106..13555
                     /locus_tag="LZB08_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBM7314275.1"
                     /translation="MGKENVVGSSQISGTWGKLWWDGTLIAEVLSFEAKVTANREEVQ
                     FGMSKDSKITSLSGEGTIKLGKVYSRGKKKLLEAWKNGEDPRSTLTSKVKDPGTPGKQ
                     AETVTINNVWFNELALSQFEKGGKIEEELSFGFTPNDSDVMDEIDEI"
     gene            13603..13986
                     /locus_tag="LZB08_000761"
     CDS             13603..13986
                     /locus_tag="LZB08_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBM7314276.1"
                     /translation="MDNKKEMVTIEDILRRKEYFAKKSEETKQLYIPSLDGNIEISKP
                     DRMLCLDAIEMEDAVEGDKYFVYEIVKSPNLKSEKLHAEFGCKDNPLDIVDVLFEAGE
                     ITDIVKIATKFAGFGVVEEIEDLKN"
     gene            14010..14177
                     /locus_tag="LZB08_000762"
     CDS             14010..14177
                     /locus_tag="LZB08_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314277.1"
                     /translation="MISHYLEKGVDLDKLTNLSMIERNFRIACMLYEEEEKIKLISEL
                     IGAMFGGVKNG"
     gene            14170..15924
                     /locus_tag="LZB08_000763"
     CDS             14170..15924
                     /locus_tag="LZB08_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314278.1"
                     /translation="MARRHIGAVISLKDNMSATMRGIRREQKQFQNEVRRTRNEMRSA
                     SRERMRIRMDATPAHRTIQDLRRKLAPLRTKLVKAVVIKDLATEKIERIKSNVKSFGR
                     FIARPAIKLKDETKGMIDKIKNRLTSLSTIVPIGAAVGAAGMAVKSGMELEQQQISMR
                     HFMGVGNKGKSSKELDGMSTNYLKDLRNNANATPFETGEVISAGTRSLQIAGGNTKDA
                     MQMVKLAEDMAALNPGKTVGDAMEALADMNIGEMARLTEFGVKASSTDDPKEVQKKLE
                     TMYAGGANKLAESGSGLLSTIMGKLKSNIADIGLGMLEPLKPVMAGLIGFIDQASPKI
                     LEVCTKITSGIGMAIGWIQQQMPTLAPIFQTAFGAVSSIVSTVVPIIGQVISALAPIF
                     MGLLSVASSVLSGIASAVKTVAPVVSTLISGFSPVFSNVGSALKSMGKIFKNIFDSVM
                     KIVKKASDFIKPLLSGIIGATKGISDGVSWVAGKLAGNATGTKYWSGGLSVVGEHGPE
                     LVSMPRGSKVFTNAESKSMINKSIPNLRQVQGGNTNYNITIPKIAETVIVREDADIER
                     ITSSLIKKIQMAKMGGVV"
     gene            15924..16562
                     /locus_tag="LZB08_000764"
     CDS             15924..16562
                     /locus_tag="LZB08_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBM7314279.1"
                     /translation="MEMWLRQSNDAFRFPILPTSFEISGSINTSTTNVLKLGEVIVCG
                     GTGLRTTEINSFFPSKQYHFCNYKDFPQPYDCVNKLKKWMEQGLILRYIITETDVNME
                     VIIESFKHGKQDGTNDVYFTLSLKEYKRIQIPKVSINNDERLSSVKDVPITKGFETKK
                     QRTHKVGKGDSLWSLAKKYYGNGDLWKKIYDANKKLIKNPDIIKDGWVLVIP"
     gene            16802..18322
                     /locus_tag="LZB08_000765"
     CDS             16802..18322
                     /locus_tag="LZB08_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBM7314280.1"
                     /translation="MIINRSKDSSNNSISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFNDNKLAIGNIPKTNVKYTKMFIGVTGYDTIMSAYTEASKTTKKKYMIEANLDKFN
                     VIEKGIVTLNVMFEEGSNLINTSFSESMENVKNKVLVVDQYGNKISEKVNDEIFKDVG
                     IIMQKVIQQQENQTIDIDSEFKGIEQTCNLKGYGDVSCITGRGVKVKDSYTGLTGLFY
                     IDTDKHNWDSNGNYEIDLDLNFQNIMDEKTAGQDEQKEESSDLNGEGTLNGREVKAEF
                     TAYYPSNNPMEGGYYQAMDGKRLVPSNNTCAAPSKLKFKTKIQAKCPGTKIDGKTYTV
                     TDRGGAIGLKNGVYRIDILMSSEKECNDFGRRKGTIIIGDGTGYTNTTGKAKELISIA
                     KSKLGCKYVWGATGPNTFDCSGFTQWCYKKIGINIPRVSRGQGKAGKAVSKGSLQPGD
                     LVFFSSKGANGTIDHVGMFIGNGEFIHSPHTGDVVKISKLSGSYYTKNYVTARRYL"
     gene            18338..18664
                     /locus_tag="LZB08_000766"
     CDS             18338..18664
                     /locus_tag="LZB08_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBM7314281.1"
                     /translation="MSDPINEFIGIIREEGKHYNEPSFFVGKIKSKLPDLKIEINNIV
                     LEKEDILIDSWMLDRQIESFDTETNQEHQHEVKNPFIDTFESGDIVIMFKIGDKFAVV
                     SKLVSL"
     gene            18664..19092
                     /locus_tag="LZB08_000767"
     CDS             18664..19092
                     /locus_tag="LZB08_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBM7314282.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILENGDF
                     KIIEGNEAIKIWVYKCIKTNRYEHEIYSWGYGTELSELIGQKYSKGLTESEASRYIKE
                     ALLVNQYILDVNIKDTKFTDDLLSVNIEISTIYGEVEVNV"
     gene            19085..20137
                     /locus_tag="LZB08_000768"
     CDS             19085..20137
                     /locus_tag="LZB08_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBM7314283.1"
                     /translation="MYSSQTYDVIKNRTLSNIDLDIYKGEGSFLSDMVSPISTELAKF
                     YIELSYLHKKAFIEDNFDDFLDKRVNEFGVYRKLGTEATGEVVFEGKAGTVIPNGTII
                     SYNELLFVVIKDIVISSEIEQNTSPVQALEIGIRYNIPASTEFKLQDEINGISKIYNN
                     LDFKGGTEIETDEELKERFYKIQRNQATSGNKAHYEEWALEVEGVYNAKIYPRWDGPG
                     TVKVLIFGENNQAVDTEVIERCKEHIEEEMPIGPALTVLTPSVLDISISASIKLEAGY
                     TLDFVKESFLESINSYLINVNKEIIYTKVSAILASIEGIHDFSNLLLNNKAENIVFEE
                     DKVPSVTTLEFSEVVV"
     gene            20137..20745
                     /locus_tag="LZB08_000769"
     CDS             20137..20745
                     /locus_tag="LZB08_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBM7314284.1"
                     /translation="MKLIDKLPSFYNNNVTSKIQEAYDIELETLRETYDDTFDQFFVD
                     TATWGLDYWENILSIKNRFDLNIEDRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNDCSYNTILELDKKIEEIKPAHLEHKFERVLFDKSRLYAGAT
                     ISAGETVTIYPYVPRDLESFGEIAICSGNDRALEKVTLYPKK"
     gene            20762..21541
                     /locus_tag="LZB08_000770"
     CDS             20762..21541
                     /locus_tag="LZB08_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBM7314285.1"
                     /translation="MAEQQYFTLVTDIGKAAIANASVTGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQVDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AVSSYPTTYKPEADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDLQDLDSKI
                     DTTKTELTNSIETAKTELNNKIGDTTQLETTDKTSLVGALNEVKTSVDSIETTAEKTS
                     YNNATSNLTATNVQGAIDEVVRKIENFNEVNISIQNDMLPI"
     gene            21559..23199
                     /locus_tag="LZB08_000771"
     CDS             21559..23199
                     /locus_tag="LZB08_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314286.1"
                     /translation="MQTEWDFNYANYVQNVSLLPGRYKLECWGACGGTLGASDWTECA
                     KGGYSKGEVILKKRTNLCICVGQSGYEKLDTGSTLTRTGFNGGGRTNAHSINGNYYSK
                     YGGGATDIRAYYTRATWDSAEGLLSRILVAGGGGGMKNNFASARSIGHGGGYVGENGI
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGGIGVGGGGGWHGGAGSYSNECGGG
                     GSGYALTKDSYKPPGYIPTSEYWLENVVMTTGGNTTRADGYAKITLLQALPFLNISSY
                     NSTTATFKADHTNPTLLTKIEYFIDDTLKETITTDLTLEKTINYTLEDNALHTLKIVV
                     TDSANATAEKNVSISKGIAPLPAGSTTDEVTSKWMEIKDTFKSGKTSIINTLALKNID
                     ASLNNTLVELSEKIKTSFDSSDASVEDLTNKLTQANNTISQLNTKYKFAYSLDLTVHW
                     FIDCGFKPDFVIYECINNHGKFLVFLTPFNYSVFDIGNLGWTITAREDISKKDSFSIN
                     NINQFWGTKIRDTGFNNADMPKDSLNDRYVFWIAIKCL"
     gene            23212..23511
                     /locus_tag="LZB08_000772"
     CDS             23212..23511
                     /locus_tag="LZB08_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314287.1"
                     /translation="MKNIGRIVFYDNDTGDILMFYGEKTVSDSIDINIPKIDSIELPY
                     GSIDYTRNRLIGINVETKEPILEEIPVYVSEEEKRIQELENQILIAENEKVGGLL"
     gene            23511..23693
                     /locus_tag="LZB08_000773"
     CDS             23511..23693
                     /locus_tag="LZB08_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314288.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNREFQLDDVTNAEYRKAVED
                     YIIEHSGVVEGAEPTK"
     gene            23729..23959
                     /locus_tag="LZB08_000774"
     CDS             23729..23959
                     /locus_tag="LZB08_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBM7314289.1"
                     /translation="MNEEVIKEKIKRNEIRINRHSDEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLIGFFIFAIQKGIF"
     gene            23976..24233
                     /locus_tag="LZB08_000775"
     CDS             23976..24233
                     /locus_tag="LZB08_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBM7314290.1"
                     /translation="MDNLISFIPEQLLILVAVLYVIGAGCKKYKQLDNKYIPVVLLAL
                     GIGFSIWMLGLNPSAVLQGVICWGISIGINQTYKQLREENE"
     gene            24233..24991
                     /locus_tag="LZB08_000776"
     CDS             24233..24991
                     /locus_tag="LZB08_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7314291.1"
                     /translation="MVDIIKMLTKKKCYPNKNSPKFIVIHETDNEDKGADAKRHAQAL
                     NNGNLEASVHYYVDDKVIYQTLDHKDGAWAVGKSYGTALVAGVTNYNSINIEICVNRD
                     GNYTKARQNAIDLTRKLMKDLNISTDKVIRHYDAKRKYCPRKMLDNPKLWVDFKDKIK
                     NDGGVNSMIKYTIVYDGEVDRIPAEVIGWNYKKEECRVCDIKEYIPGQTQNLYIVGGG
                     ACNKIGSITKEHYTMIKGNDRFETLYKALDFIKK"
     gene            complement(25022..25228)
                     /locus_tag="LZB08_000777"
     CDS             complement(25022..25228)
                     /locus_tag="LZB08_000777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314292.1"
                     /translation="MKDVFEELISIKDACELYKKGESTLRLNIRNGKFVEGVDCKKFG
                     TTWVFKKTALEREYNKKDKKKREW"
     gene            25382..25852
                     /locus_tag="LZB08_000778"
     CDS             25382..25852
                     /locus_tag="LZB08_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314293.1"
                     /translation="MNKQKARRFLRVIDMNIDKIEEEAIKAFKESCLIKETNDIKIYI
                     DIQGKVEAIAVQTWAKLLGDDKEINIFTLNQAPTHLDDMFGEIYHVNDYEEFEKWCEK
                     EDKSLNWDSYEKFNKENFEEIAERNIDDSSSAFLEKLHESIENCKQELQNIVEN"
     gene            complement(26163..26444)
                     /locus_tag="LZB08_000779"
     CDS             complement(26163..26444)
                     /locus_tag="LZB08_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314294.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVVNFLRYAGFDKDMKIKL
                     FQTIIEAVARKKVSLPSDSEFTEGILQSDEAEMTMNVGFLIEGLSYHNN"
     gene            complement(26588..26920)
                     /locus_tag="LZB08_000780"
     CDS             complement(26588..26920)
                     /locus_tag="LZB08_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314295.1"
                     /translation="MPSFNRDLLVDKEKPILKNGTIAQNDSTTTDNKTLNIYNLVNKK
                     SKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNERN
                     RRNKTKKK"
     gene            complement(26921..27730)
                     /locus_tag="LZB08_000781"
     CDS             complement(26921..27730)
                     /locus_tag="LZB08_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBM7314296.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNENYINVDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIIPIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTILVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFTNMLEELKRKGVL"
     gene            complement(28292..29014)
                     /locus_tag="LZB08_000782"
     CDS             complement(28292..29014)
                     /locus_tag="LZB08_000782"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBM7314297.1"
                     /translation="MFNSVKRFLKTLKEPVLTEFDIAKQYDFSKELSSGIVSSFKSQK
                     EIEYEKFIKFTNSKNKVLEHYIVFDVETTGLDAFKEDIIEIAAIKFERDVPSEIFSTF
                     VKPSKSIRKKITDITGITDEDVVNAPSIEEVLPHFLNFISDYTLIAHNAIFDMEFILD
                     KLYKNGYKKITNKVIDTLTLSRKYIRTYEGKKLKSYSLPSLHEELGLNYSSHRALEDC
                     KSCASVYKACKSEMKFKDELVY"
     gene            29459..31612
                     /locus_tag="LZB08_000783"
     CDS             29459..31612
                     /locus_tag="LZB08_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBM7314298.1"
                     /translation="MKNNINKLFLEECNAERAEHEKENYSNFNRHETYEIKKYINEKK
                     LGIKTAIANALDGGKTLFIAPTGAGKSYSFINTLKKLKTKALFILPNASNVEQAMHEY
                     NIAGAYDKIPAKQALENNNLAVMTWDKTEQLIDVDLREYIIVVDEIHQTYTDSYRGKA
                     IKNLNNIMSKCKGRIDITATPTKLEFEIYDYIIEYTQKQKTEYDVKLYNDFDNKNFTE
                     IINIINKSNNSAMLMNDISTLEFIRDSVNKNAGVVYADGKEENELYSRIVKNSDMKGY
                     ETLLNTTTILAGVNINNKDITDIIIVNIKDIGAIKQYVARFRNLKKVNVHIFNKYKEE
                     CNVYKIEWLVDKNIEKATILKDAYNKVSKHTLMFETVGINATPIRIDSNVYYCMKDNC
                     YKVDKLYIKSQIYSNYYNTRTIQSFKVLLEEYFDNVNITDAKEIETNEKELKEYKKVV
                     KEIKEATRNILKEHKEILVGYRQIKSNSKSFSLMQYHNDMKLSSKGCLEAYRAYDIHN
                     LVKKSKSNSMLELYSNYVLDNKFSLDLAWKLANTANRKRGAIFNKLNTLIYRELKEEY
                     PEFLNDELIQVTVFNYIDKLFGIGTSYTELHLQELSDDLRLILGENWNLTTKKLSIIL
                     NEVFVIEKKKYRGGNSLENIFFYKNINPKLFPLEAERMQVNNIKRYISIDDIKKDLKL
                     DEKDKSLEHAIKHTKDRMLNSLDEDEKLLLLKGFM"
     gene            31633..31971
                     /locus_tag="LZB08_000784"
     CDS             31633..31971
                     /locus_tag="LZB08_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314299.1"
                     /translation="MKLRIGIIDKYVAEASYELSELLIDAFNKYDEFVEAIRGGEIRI
                     VPFNLGFQALLEEDSTYDEYEFYYDDEDDKCFFHIKYRNVKDVELSKELLLELIKVNL
                     PAGAKLIVNN"
     gene            complement(32445..32798)
                     /locus_tag="LZB08_000785"
     CDS             complement(32445..32798)
                     /locus_tag="LZB08_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314300.1"
                     /translation="MNIRIRKLRSSEGLKQIEFGEKLGKTKHEIYNLESGRTKVKESD
                     LKLIISLFKVNENWLRTGTGEMYNTEDRNSVKAEAFCAIDENEKLAKAVLEFSKLTDE
                     QLESILKILEVFSKE"
BASE COUNT        13107 a         3742 c         6223 g         9977 t
ORIGIN      
        1 aaaaaaaaca aaaagatatt gattgtataa taaaggctaa taacattgga aatatcgcta
       61 atgaacgtat tttagacata gataaattga acgaggcagt cgaagaaatg aaaaaaatga
      121 tttgttaaaa aagtgtactt gacggctaca ctaaaaaatc taatttaaaa aaactaaagg
      181 aaaaggtgag attatgtata ttgaaaatat agaagaatta aaagagttaa ttgaactttt
      241 tggagttgta gaagcgaaga gtgaaaacgt agttgcgttt ggagaggttg aaagtcttga
      301 ctcttgtaca gaagaaaatg cagagaaagc attagatata ttaaataagc ataaacaaga
      361 gaataaaaaa agatatagag atttctttga attgaaagaa tggattaacg gagatgaaga
      421 acgaaagcaa atatttgcgg atattgctat ttgtgaaaga tgtggtaaaa tagttgctaa
      481 ttctgaaatg ttaacctcgt ttcctatctt agcaaaatat gatgagaatc gaagtgtttt
      541 tgaacagttg gaaactgttg ctatatgccc agactgcttg atgtttataa ctagagtata
      601 tgagtcgtta tttgatttcg aaggttagaa acaaaaagta acaatgttag tcgtcaagag
      661 tgtttttaat agcattaaat tagttaaaat aagtatctta acatatgtaa caaggagtaa
      721 aacaaatgaa gaagatttca gaaatgagta tattggaaaa ggctgatttt attagaaata
      781 gacaactaga attgaaaaag aaatatcaaa acaaggctaa aggagaattg tttaatatgg
      841 cttgtagaga gttagaagaa aaagaaaagt gcgaaggggt gttttgatga tatgttgggt
      901 tataagcttg taagaactga agaattgtta aagcttagaa aagacttaga gataaaagaa
      961 gaagaattaa aaagaaacct tgaagaacta tctgaacaac atttatctaa agcaagtttg
     1021 tatatgaatc taggaatgac tataacagaa aatgaatttt ataattcaat aatcgagaaa
     1081 ttgtttgata aagattttag aaaagctatc agcattttta acaaaactaa aagtattcgg
     1141 ataaagaata aaaaagcaac tgaaatatta cttagaattg atagagaaga aaaaataaag
     1201 gaatttcctt gtagaaataa aagaaggaag tgttagaaat gaataatgtt tgtttagttg
     1261 gaagattaac aaaagacccc gaatttaaat atattccaaa atcgggtacg ccagtagcaa
     1321 cttttacaat tgctatagat agagactatg taaataaaga aggacaaaaa gagactgatt
     1381 ttataccagt tgaagtaatg gggaaatctg cggaatactg tgcgagttac ataacaaaag
     1441 gtcgattagt cgctttgaaa ggtgaaataa gagttgattc atatacagat aagaaaactg
     1501 aagaaaaaag gatatttacg aaagttcata caaagatggt aacgtcttta gaaagtagta
     1561 aaaataataa agaaaataag gaagaaactc tgaatttaca agctatatac gacgaccccg
     1621 atataccgtt ttagcagctg aaagaaacag agaaaaagct ccgttttgga attaaaaaag
     1681 gatgtgggaa aatgactaga aaaggaaaag tattaaaaat ttgtggttgg tgcggaaaaa
     1741 catttctttc tgataatgat ggtaaaattg catattgttc taagaaatgt aagaaaaaaa
     1801 gagaaaaaaa tttagaaaca gaaggggcaa aaaaatgaaa gagtataaaa ttgaaattga
     1861 agaaacaata tttttaaaac ataaagttat tgttgaagtt ctagagtgaa aaaaagctgt
     1921 aagaaaataa aataaggaga aaaaagaatg gaaaatattt tttggtggag ttttatggat
     1981 ttagaagaag cggaaaaagt tgttacagaa gaaagtataa aagacttaga aaatgctata
     2041 aatgaaattg agaatgaagc ggaagaagtt aaaattttaa ttattaagaa aaatggcgat
     2101 gaaaaatata ttaatttcaa acacgatgga tatatgaaag acgttataaa agatagaata
     2161 gttcaatgtt ttgaagaaat aaaaataatg catgaaacaa ttttagaaga aatataaaaa
     2221 gggagaaaat aaaagatgaa ttttaaaatg attgctaatg tgccggagag ctggacagtt
     2281 caaaaacaat ttgaaaagat attggaagaa attttagaac ttaaagaagc tattgcgtta
     2341 gatgataata agaaaatatt agaagaaggt ttagatgttt ttcaagcaat tctcacttta
     2401 tttaaaataa ttggtataca taacataagc gaagggttga aagagcataa caaaaaactt
     2461 agaagaagga aatggaagct agaaaaaata gattaaagtt aaaggtatga ataagtggca
     2521 tttactttaa ttattaaata gataagatag aaataattta tattaataga ttttatttga
     2581 acttaaaaaa atgaagtctt gtaacaaaaa aacaaaataa aaatccagaa tattttgaat
     2641 aatatgcagt agttggaaat gctacaaaat acctaactag tcccaaaagc taaatttaag
     2701 gattagttag gtatttataa caaaaagtgt aagaggtgca aaaatgaaaa taaaaaaaga
     2761 gtatattgac aaagttgaaa atatattaaa aatgttagga gatgaatata gaaagttaag
     2821 agtattaaaa aaagaaatag caattcttaa acacaaagaa aactatagag aaattaatta
     2881 tgaagaattg gggtttaaag tgcaaaaaag tgtaaaaagt atagatgaca tggttataac
     2941 aatagaggat gttatctaca acaaagaaac tgaaattgaa attatagagc gaaagctaga
     3001 gttttataat atttacttaa aagaattatc agagcttgaa cagaagatga tagaattaat
     3061 atatttttat aattgggatg aaaaactacc tatcacaaaa atagcttatg aattaaatta
     3121 tgatagaagt agtctttata acaaaaaagc tattgctatt aataaaatag ctttaatgat
     3181 atatggtgat gaagctttgg agtaaacatt atttttacag aaaaaagact atttttatac
     3241 ttcgtttcta aagaaagtat gttagatagg cttttttatt gctaaaaaag gaagagggtg
     3301 agttgattgg ttgaattgaa aaaagagtac attgatgaag ttataaaact tttagatgaa
     3361 gttagagaac ttagagtaag aaaagaagtt ttaaatgatg aaatagaatt tcttaaaaag
     3421 aatgaaaagc ttagtagtat taattttaat gaacttggtt ttccagtgcg ctccggaagt
     3481 tatagtatag atgatatgat tataaattca caggagaaaa tttatataaa agagactgaa
     3541 attgaaatta tagatagtag aatagaaatg attaatattt acacgaaaag attgagcgaa
     3601 gaagaacagg agattataag tcttagacat tttgactcaa aaataaattc ctacggggaa
     3661 atttctgaat tattaatgat ttctaaaacg gtcgttcaga ggaaatatac ggacgctttg
     3721 aggaaaattg tattgatgaa gtatggagaa aaagcgaaaa aagataggga ataaaaagga
     3781 ctaaaaaagg tcaacacaaa acttttgtta catgttataa ttgtattgtg gaaaattaaa
     3841 tatgtttcta cttcaaaagc ttgtgagaaa aaactcatga gcttttttta ttctcttttg
     3901 aaagggggta ttttgattag gagtaaaaag agcatcgaag cctatcaggc gtacacaaga
     3961 agtgctagat attcaagaat atctaagata taaaagttat agaaactatg tgatttttat
     4021 gctagggata acaacaggat atagagcagg agatttagtt aaactaaaag ttagagacgt
     4081 aagggaagct ttaaaaagac aagaatttac tattatggaa ggtaaaaaag ctaatagtaa
     4141 aaacattagg gaaaagaaca gaaaacctcg tacagtagaa ataagaccta ggattgcgca
     4201 aattttaaag aaatatataa aagataagca tgactatgaa tacatgtttc catcgagaaa
     4261 aagaaaatat cctcacattg gtgttgaagc tgttagcaaa gctttaaaag aagcaggaga
     4321 atattttggt ttatatgata taacagcaca tagcatgagg aaaacttatg cttataaaat
     4381 ttatattgat agcgaaaaag atattgttgc agttaaagaa ttgttgggtc actcttcgat
     4441 agaagaaaca aaaatgtatt taggtttaga taaagaattg tatcatcatt acagtgagtc
     4501 attagacgac tttgtaaggt gatatttttt tatttgtctg tttgaatgtc taaaaatttg
     4561 gtgtagtgat attcaaggta tcaaaatcac tatataagaa gtaagaaaaa aatagattga
     4621 atgtctggtt ctctaagaaa ataagacgtt caaatgaaga aatacgaact atatttaatt
     4681 agtttttaat aatgttcgta tagaaaaaga ggtgttttat gaagaaaata gagttggatg
     4741 ataaagagat taaactgatt atttcagcat tagatttaaa agtttctcgc aacttaggca
     4801 aagcttataa aaacttccca tatgcttttg atgaaaaaga agagtataag agggatgtgg
     4861 caactatgga gcttttgatt gataagtttt taagtaagct taatgaggta gagtgtgagg
     4921 gagtgcttat ataatggcta gagagtttag tcgaagcttt tataatagca aagcttggaa
     4981 ggagtgtagg caatcaatta ttaagaaata tctaggcttg tgtgctgagt gtgggaagct
     5041 aggagaagaa gtgcatcata taaaatattt aactcctgct aatatacatg atgttgagat
     5101 aactttaggt gaggagaatt taatattgct atgtaaagac tgtcatagta aaaaacataa
     5161 gagtaagaaa gacattacta gaacagggtt aaaatttaat gaaaaagggg aattgatttc
     5221 gatttagaaa aaaatagtat atcccccctt aaaaacgacc ctgggggctg atttcaaata
     5281 ccgatgtccc cacatcaatt ttcctccgca tgaaaaaatc agaatgggag gggggttatt
     5341 taaaataatt tacgaacaat tagagaaaga aaaaaagata aaacaagagg taagcagatt
     5401 aaagaaaaac tataaagatt tagaaaaaga aaaagttaaa attttagatg ggctaataaa
     5461 tgaagcagct tttttaaaaa tatctttaga agggactagg gaaattttga caaaagaagg
     5521 tttgactgaa atttttaaac aaggtaagca ggaatttgaa agagagaggc ttcaagttaa
     5581 aatatattta aattttatga agctttactc tagtgttatg aaacaactga ttgatattat
     5641 tccaagcgac aaaaagcagg aggaagagga caaacttatt gagtttatga aaaaaggtag
     5701 acttcaaaaa tgacatacat tgaagagtat tatcaaaaaa tattaagtgg agaaataatt
     5761 gcttgtagca ggattaagca ggtatataaa aaacttgttc aagacttata taatccaaag
     5821 gataattggg tgtttgatga agaacttgcg aatagaccta tcgagtttat agagacgttt
     5881 tgcaaacaag cacaagggaa attaggggag cctttgaggc ttgaattatt tcagaaagct
     5941 aaacatcaag ctgtgttcgg ttttgttgat aaagagactg ggtttagaaa atatcaagaa
     6001 gtacttgata ttcgaggtcg taaaaatggt aagactacag aattggcagc agatgaatta
     6061 tttatgttaa ttgcagacaa tgaagggtcg ccagaggttt acaatatagc aactaaatat
     6121 gagcaagcac aaaaagggtt taaagagtgc tataaaatgg tacagcaatc taaaatttta
     6181 tctaagcata ttaaaaaaag aaagtcagac ctctatttcc atgctaatta tggttttttg
     6241 caggcactcg caagcaatag taatggactt gatggattaa actcacacat ggtaacaata
     6301 gatgaattgg cagctataaa aaacagagat atttacgact taatgaagca atctatgggt
     6361 gcaagaaatc aacctctttt aaactgtata actacaaatg gttttgtcag agagggcatt
     6421 tttgatgcgc aatatgagta tgcttgcaat gtcttggatg gaaaaataaa agatgatagg
     6481 tttatagctt ttatttatga gctagatgac aaagatgaat gggatagaga agaatgttgg
     6541 ataaaagcca acccaggttt aggcactata aaaaaacttg attttttaag agattgtgtt
     6601 aataaagcta aaacagaccc aagttttaaa cctaccgtta tggtgaaaga ttttaatatg
     6661 aaagaaaatt ctgctactgc ttggttgagg tgggatgagt taaacaatga aactaaattt
     6721 aatgtagatg aaatgggatt tagatatggc ataggatgtt ttgatttagc tgaaactaca
     6781 gaccttgcat ctgctaaagt tttattaagg aaaagacatg atgataatat ttatacgctt
     6841 tctatgtatt gggttccgtc tgaacgatta gagcaaaaag ttgatgaaga taaaattcca
     6901 tacgatttgt gggaaaaaca aggtttgtta agggtatgtg agggaaataa aataaatcca
     6961 tacgacattt tattatggtt tagagaaatt agggaagaat acgatattta tatttcttgg
     7021 attggatacg acccttggca tgttgattca agtttacttt tagcttatga aaatgagttc
     7081 ggaaaggatg caatgattaa agttaggcaa ggagtttata cactatcagc tcctatgaag
     7141 gagctacggg cagatttaaa agctaataaa gttatttata ataataaccc tatcgataaa
     7201 tggtgtctta gcaatataga aataaaaaca gatataaatg gaaatataca gccaataaaa
     7261 ggtatggaca gaagacgacg cattgatgga ggtgtcactt taattatagg ctatgttgtt
     7321 ctaaaagaaa aaatgtcaga atatgaaaac atgatttaag taagggggtg aaaaatgaac
     7381 atatttaaat ctaagaagaa aaataaagaa gctcctgaaa gaattgtaat ggaactcatt
     7441 tcagatttgg gaaacgggtt ttatagttgg catggcaatt tatatagaag cgatattgta
     7501 agaagtatta tacgaccgaa ggctaaagct gttgggaaaa tggcagctaa acatattaga
     7561 agtaatgaga ctgaatttaa aactaatcca gagccttaca ttaaattttt gcttgaaaat
     7621 ccgaacccat ttatgagtgg gcaaatactt caagagaaga tggttactca attagagctt
     7681 aatagcaatg cttttgctgt gattattaaa gatgatgata atataccaac tcaaatttac
     7741 cctttgaatg ctttaaattt tgaagctatt tatgaagata gagttttgtt tttaaaattc
     7801 ttacttagaa atggaaaagt agttacctat ccatattcga acataatcca tttaagaaaa
     7861 gattttaacg aaaatgattt atttggaacg cctccaacta aagtacttaa agcgcttatg
     7921 gaagttgtaa atacaacaga ccagggagtc gtgaaggcta ttaaaaacag caacacaatc
     7981 aaatggttat taaaatttaa aacatcactt cgacctgacg atattaaaaa agaggtcaaa
     8041 gagtttgaaa aaaattactt acaaatagac tcggaagcag gcggagctgc cgcaactgat
     8101 tcaaaatatg atgctgaaca ggttaaagct gagagttatg ttcctaacgc cgcacagatg
     8161 gacaaaacta cacaaagatt atattcgttt tttaatacaa atgagaaaat aattcaaagt
     8221 aaatataccg aagacgaatg gaatgcttat tatgagtctg aaattgagcc agtaggattg
     8281 caactatcta aagaatatac gcaaaaatta tttactagaa aagaaagaag ttttggaaat
     8341 gaaattattt ttgaagcttc taatttacag tatgcaagta tgtctactaa attgaatttg
     8401 gttcaaatgg ttgatagggg ttcgcttgct ccgaacgaat ggcgtaagat aatgaacctt
     8461 tcgccaatag agggcggaga caagccagtt cgccgattag atactgctat tgtggaaggg
     8521 ggtgattaaa ttaaatggct agtgataatt tgaaagaatt tttaaaagtt aaaaactcta
     8581 cagaaacaaa tgcagatttg tttttctatg gtgatattgt ctgtgatgag tgggatgcct
     8641 ggacagaaga agaccagtat ccacttgcaa taaaagattt tttagcacaa gaacagggaa
     8701 aagatttaaa tatttatatc aattccggtg gtggttctgt atttgcaggt atggcaatat
     8761 ataatatgct aaaaaggcac gaaggattta aaactgttta tgtagatggt attgcagcga
     8821 gtattgcgag tgttatagct ttagcaggtg atagagttgt aatccctcaa aatgcctatt
     8881 ttatgattca taagccttgg ataggtttat gtggttctta taattcagat aaattaatta
     8941 aggcggcaga ggatttagac agaatagagg aaggtatttt aaatgtatat caagataact
     9001 taaaagaaga aattgatatt gaagaaataa aagaaaagct aagtgaagaa acttggttta
     9061 ctggtaaaga agcatcaaat tattttaatt ttgaagttga tgagaaaaaa gaggttgctg
     9121 cttgcgtgag cgattatttt gataagtata ataaagtacc tcatgcttta aaaaataaaa
     9181 tagataagac tagtaataaa aaagaaaata ataataaaaa aagagttcaa ctgaaactga
     9241 acttgttaaa attagggggt ttaaatgact agagaagaat attttaagaa aagacaagaa
     9301 atgatagacg aggcgcaaaa attacttgat gatgaagttg gggaggaagg aacaggagaa
     9361 gaaaaaacgg aagaagctga aaaaatagct aataaaataa aagctttaga cgaggaatat
     9421 gaaagaaatg taaaagctag ggcgaattta agagcgttgc aggacgattt taaagtcaac
     9481 cctactattt ttaatttaac taataacaaa ggtaaaatag aaggtataga agacacaact
     9541 gttaaggata aacaagaaca atataaaaat gcttgggcta aagatatgtt aggaaagccg
     9601 ttaagttcag aagaacagga aatatttaat agtattaacg cagagtatag agcggaagtt
     9661 caaacaagcg aaaataatac tattttaatt cctaaaactg tagcttcggg tatatggaaa
     9721 gaaattggtg acatgtatcc tttgtttggg gatgcttctc caacttttgt agcaggtgat
     9781 ttgacgatta tagcggaaga agacggtggt gatgatgctg cgtggtatga tgaagaaaca
     9841 gaagttaaag aggacggtta taaactaaaa gaaataactt taagaggttg tgagcttgcc
     9901 aaggatataa ctgtatcgtg gaaattgaaa aaaatgagta ttgatgaatt tgttccatat
     9961 ataactagtc ttttagctga aaaaatggga gcagcattag caaaagctat tgtagatgga
    10021 aaaggaaaac caggggaaag tgattctttt aaaccccaac cacttggaat aaaaactgct
    10081 ttagcaaaag aaactagtaa ggcgcaaata atagaatatg ttgacaagat agcttataca
    10141 gatataacaa aattaatggc tgttttgaaa aaatggagta atggagcttg tatttatgct
    10201 aatagtacga cgatatggac gcaattagcg gaaatattag acacaacagg aaaaccgatt
    10261 tttatccctg atgctgtaaa tagtgatggg gttggaagaa tgtttggtag agctgttaaa
    10321 atggatgata gcatggcaga tggcgagatt cttgctggaa atatagcaaa aggttatgcg
    10381 ataaatataa atgagaatgt aactttatat acagatgaac atgtaaaaag tagaaaaaca
    10441 gattatttaa cttattcatt agttgatggc aatgttataa gtaataaagc ttttggtatg
    10501 atagttaaaa aaactagtgc agttgcgaag taggtgtttt gaatgattgt atcattagaa
    10561 gaaataaaag aatatttgag attagaggca gattataaag aagatgataa tctgcttttg
    10621 tcttttctga aagcagcaga ggaggatttg gaaaatcgaa caggaaaagt ttttaatgaa
    10681 actaacaaat ctgaacttgt tagtttgtat gtaaaaatgt atgttgcaga gcaatatgaa
    10741 aaaagaggtg caacagaaag taatagcgaa aaagttagat ttgttttaga aagtataata
    10801 tctcaaattt ctatatgtag taggtactaa aatgaatgtt ggaaaattaa ctcagagaat
    10861 agaaatacaa atttatggag aaattgaaaa cgacatagga gaaattacaa aaggatggtc
    10921 tacgtataaa aaactttggg ctaacaaatc gttgcttaga aatagtaata attatgtgtt
    10981 agataaagaa aatatagagt attcttacag atttaaaata agatatagaa ctgatataac
    11041 agaagctatg agaatagttt gtaatgatgt tgtctatgat ataaagcatg taaataatat
    11101 aaaagagcta aataaatatg aaacaaatat tgattgtatt ctttataaag aaggtgttta
    11161 caatgagtag tagtacagat tttaatacaa atggtttaga tgattatact aataagttat
    11221 ttaagcgtat tgcaaaagaa taccccaaaa aagctgaaaa gcttatgaat atcagcttag
    11281 gaaaatgcaa aggagaagct attgcaagaa ctccgaagtc agataaaaag cctaaaaaat
    11341 ataaaagagc taagcacatg aaagataatt ggaaaactaa agtgcagtct aaaaatggga
    11401 attttgtagg ggttttgaaa aatgattctc cacatgcaca tctaatagaa aatggctggg
    11461 tgacaaaaaa tgggggatat gttgaaggaa cacatatgtt gcaacaaacc atggagcatc
    11521 aaagggtaaa aatcgataaa agaatagaaa aaatggttga cgaaaccttt aatctttaga
    11581 gggggtaaga gtgttaaaaa ttgtttctgt aaaaaaagct atagttgaaa agcttaagtc
    11641 tttaaatata aaaacagtag caaatgaaat aagaagcggg tttaaaaaac ctgctttttt
    11701 tattcaaatt attcctattg aaatggctag cgacccaagt ttttcaaata gtacattact
    11761 tgttaatata cattattttt ctgaagaaaa gacagagtta gaaaatttaa aaatgattga
    11821 taagcttaat atattatttc aagattgtat tttagaaatt gatgggggcg aattgactat
    11881 agaagaaaaa agtgtagaaa tatatgagaa tgttttacaa tacaaattta atttgcaagt
    11941 agttgaaatt atagaagaag acgaaagtaa atatgagctt atggaagagc ttgaaatgaa
    12001 tatttaaaaa aaggaggttt tgttttggga ttaccaagtg cgataattga gtttcaaaga
    12061 cgttcaagga ctgttaaatt tagaagtcaa aaaggaattg tggctttaat acttaaagat
    12121 tcaacagcta caaagaaatc ttattctatt gattttttaa cagatataaa cgaagctgaa
    12181 tttacaaaag aaaactatga ttatataagg cttgcatttt taggaaaacc tagcaaggtt
    12241 attattgaag ttattaatga ttcagctgat gttgaaagga ctttagacga tgttttaaaa
    12301 gctttaaagg cgaataaatt taactattta gctattcctt ggataagtga agaagctgat
    12361 aaaactaaaa tagtcaactg gataaagaca gctagaaggg aaaaagagat atacaaagct
    12421 gtgctaccca atattgcaga tgctaacgag aaagcaatta taaatttcgc aacaacaggc
    12481 ataaaagttg gggaaaaatc ttatacgaca gcagaataca cagctagaat tgcaggtatc
    12541 ttagcaggta taccactttc agaaagttgt acgtattttg ttttagatga agttacagaa
    12601 atagaaccaa tcgaaaatcc tgatgaaacg ataaataaag gggagctaat tttaataaat
    12661 aataatggaa taaggatagc taggggtgtg aactctttag taactttaag taaagaagat
    12721 acagaagact taaagaaaat aaaaatagtt gaagctattg acatgataca ggacgatatt
    12781 cttcaaacct ggaatgagaa ttacgttgga aaagtaacta acaaatatga taataaagta
    12841 ttgtttctat ctgctataaa caattatttt aaagagttac agcgtgatga agttcttgat
    12901 aatagtcaag aagcttatgc gcagatagat atagaagcgc ataaaaaata tctgaaagaa
    12961 gcgggaattg attatagtga aatgactgaa cagcaaataa aagaagctaa tacgggttct
    13021 tatgttttta tagaaggaaa cattactgtt actgacgcca tggaggactt gaaatttaaa
    13081 atatatatgt aaagaaggtg agtagatggg caaggaaaat gtcgtaggaa gtagtcaaat
    13141 ttccggcaca tggggaaaac tttggtggga cggaacttta atcgctgagg tgcttagctt
    13201 tgaagctaaa gttacagcaa atagagaaga agttcaattt ggaatgagta aagattctaa
    13261 aataacatca ctaagcggtg aaggaactat aaagcttgga aaagtatatt caagaggaaa
    13321 gaagaaattg ctagaagctt ggaagaatgg agaagaccca cggagcacac ttacaagtaa
    13381 ggtaaaagac cctggaacac ctggaaaaca agcagaaaca gtcacaatta acaacgtgtg
    13441 gtttaatgaa ctagctttat cacagtttga aaagggcgga aaaatcgaag aagagctaag
    13501 ctttggattt acaccaaacg attcagacgt gatggacgaa atagacgaaa tttaaaggat
    13561 agtttttact atcctttttt tatataatag gaggatttta aaatggataa taaaaaagaa
    13621 atggtaacaa tagaggatat tttaagaaga aaagaatatt ttgcaaagaa aagtgaagaa
    13681 accaagcaat tatatattcc ttcgcttgat ggaaatatag agatttcaaa gccggacagg
    13741 atgttgtgtc ttgatgcaat agaaatggaa gacgcagtcg aaggagataa atattttgta
    13801 tatgaaattg ttaaaagtcc gaatttaaag agcgaaaaat tgcatgctga atttggatgt
    13861 aaagataacc cacttgatat agtcgatgta ttatttgaag caggtgaaat tactgatatt
    13921 gtcaagattg caacaaaatt cgcagggttt ggggttgtag aggaaataga agacttaaaa
    13981 aactaattaa aagcgatgtg gaaatgcaaa tgattagtca ttatctagaa aaaggtgttg
    14041 atttagataa attaactaat ttatctatga tagaaagaaa ttttcgcatc gcttgcatgt
    14101 tatatgaaga agaagaaaaa ataaaactta tttctgagct aataggtgct atgtttggag
    14161 gtgtaaaaaa tggctagaag gcatataggt gcagttatat ctctaaaaga caatatgagt
    14221 gcgaccatga ggggaattag aagagagcaa aaacaatttc aaaacgaggt tagacggaca
    14281 cgtaacgaga tgcgctcggc aagtagagag cgtatgcgca taaggatgga tgcaactcct
    14341 gcacacagga ctatacaaga tttaaggcga aaacttgcac ctcttcgtac taaacttgtg
    14401 aaagcagttg ttataaaaga tttagcgact gaaaagatag aaagaataaa atcgaacgta
    14461 aaatcttttg gaagatttat tgcaagacct gctataaagc ttaaagacga aacgaaagga
    14521 atgattgata aaataaaaaa tcgacttact agtttatcaa ctatagttcc aattggtgct
    14581 gcggttggtg ctgcgggtat ggctgttaaa agtggtatgg aactagaaca acaacaaata
    14641 agtatgcgtc attttatggg agttggaaac aaggggaaat ctagcaaaga gcttgacgga
    14701 atgagcacaa actatttaaa agatttaaga aataatgcaa atgcgacacc atttgaaact
    14761 ggagaagtca tatcagcggg aactcgttct ttgcaaatag cgggtggaaa tacaaaagat
    14821 gctatgcaaa tggttaaact agcagaggac atggcggcac taaatccagg caagaccgtt
    14881 ggagatgcta tggaagcact tgcggacatg aacattggag aaatggcaag acttacggag
    14941 tttggagtta aggcaagcag tacagacgat ccaaaggaag tacaaaagaa acttgaaaca
    15001 atgtatgcag gaggagcaaa taagcttgct gaaagtggtt cagggctact ttctacgata
    15061 atgggtaagt taaagtctaa tatcgcagat attgggcttg gcatgttaga acctcttaaa
    15121 ccggttatgg ctggtctaat tggatttata gaccaggcaa gccctaagat actagaagta
    15181 tgtacaaaaa taacgagtgg tatagggatg gcgattggat ggatacagca acaaatgcca
    15241 actttagctc caatttttca aacagctttt ggagctgtat cttcgattgt atcaacagtt
    15301 gtgccgataa tcgggcaagt tataagtgcg ttagctccga tttttatggg attactttct
    15361 gttgcatcgt ctgttttatc aggaattgcc tctgctgtca aaactgtagc tcctgttgta
    15421 agcactttga tttctgggtt ttcgccagtt ttttcaaatg ttggaagtgc tttaaaatct
    15481 atgggtaaaa tttttaaaaa tatttttgat agtgttatga aaatagttaa aaaagcgtct
    15541 gatttcataa aaccattgct cagtggaata ataggtgcaa caaaaggcat tagtgatgga
    15601 gttagttggg ttgctggaaa actggctgga aatgcaactg gaacgaaata ttggtcgggc
    15661 ggactttctg tcgtaggtga acatggaccc gaacttgtat ctatgccgcg tggtagcaag
    15721 gtttttacaa atgcagaaag taagtctatg attaataaaa gtattcctaa cttacgacaa
    15781 gtgcaaggtg ggaatacaaa ttacaatata acaattccta aaatcgctga aacagtaatc
    15841 gtaagagaag atgctgacat tgaaagaata acatcaagct taataaaaaa aatacaaatg
    15901 gcgaaaatgg gcggtgttgt ttaatggaaa tgtggcttag acaatcgaat gatgctttta
    15961 gattcccaat acttccgacc tcttttgaaa taagtggaag cataaataca agcacaacaa
    16021 atgtactaaa gctcggagaa gtaattgtct gtggtggtac aggacttaga acaacagaga
    16081 taaatagttt ttttccaagt aaacaatatc atttttgcaa ttataaagat tttccacaac
    16141 catatgattg tgtaaataaa ttgaaaaagt ggatggagca ggggttaatt ttaaggtata
    16201 taataactga aactgatgtg aatatggagg ttattattga aagcttcaaa catggcaagc
    16261 aggatggtac aaacgatgtt tactttacat tgagtttaaa agaatataaa agaatacaga
    16321 tacctaaagt aagcattaat aatgatgaaa ggttatcttc tgtaaaagat gtgccaatca
    16381 caaaaggttt tgaaactaaa aaacaaagaa ctcataaggt aggtaaaggt gacagtcttt
    16441 ggagtttggc aaaaaaatat tatggtaatg gggatttgtg gaagaagatt tatgatgcaa
    16501 ataaaaaatt aattaaaaat ccagatatta taaaagatgg ttgggtattg gtaattccat
    16561 aggctggagg tgatttataa ttaacaatat aaagttaaaa gtacacataa aaaatggcaa
    16621 tatctatgat ataactgata tagtagaaaa agttacatgg tcaggtgatt ataagtcacc
    16681 ttccagaaca ttagaatttt ctatagtaca atcctctttt gatgtgaatt ttcaacagat
    16741 agatatacct atagctagta cagtttgttt ttatgtggat gataaagaac tctatagagg
    16801 aatgataatt aataggtcta aagactctag taataatagt attagttttg tatctaaaga
    16861 tatgggattc ttgttaacgc agagtgaagt gtcatataac tttaaagata aattggttga
    16921 agatattgca aaacaggtat ttaatgacaa taaacttgca attggaaaca tacctaagac
    16981 taatgttaaa tatactaaga tgtttattgg tgtaactggt tatgacacta taatgagtgc
    17041 atatacagaa gcaagtaaaa ctacaaagaa aaagtatatg atagaagcca atttagacaa
    17101 gtttaatgtt attgaaaaag gaattgttac actaaatgtc atgtttgaag aaggttctaa
    17161 tcttataaat acaagctttt cagagagcat ggagaatgta aaaaacaagg tcttagtagt
    17221 agaccagtac ggcaacaaga taagtgaaaa ggtcaatgat gagattttta aggacgttgg
    17281 aataataatg caaaaggtta tacagcaaca agaaaatcaa acaatagata tagatagcga
    17341 gtttaaggga atagagcaga cttgcaatct gaaaggatat ggtgacgtaa gttgcataac
    17401 tggtagaggt gtaaaagtta aggacagcta tacaggtctt acagggctat tttatataga
    17461 cacagataaa cataattggg acagtaatgg aaattatgaa atagacttag atttaaattt
    17521 tcaaaatatt atggatgaaa agacagcagg acaagacgag caaaaagaag aaagttctga
    17581 tttgaatgga gaaggtacgc taaatggaag agaagtaaaa gcagaattta cagcttatta
    17641 tccgtctaat aaccctatgg agggtggtta ttatcaagcc atggatggta aaagacttgt
    17701 accttcaaac aatacttgtg ctgcacctag taaacttaaa tttaaaacaa aaattcaagc
    17761 aaaatgtcct ggaactaaaa ttgatggtaa aacttataca gtaacagaca gaggaggagc
    17821 gattggctta aaaaatggag tgtatagaat agatatatta atgtctagtg aaaaagaatg
    17881 taatgatttt ggaagaagaa aaggaacaat aataattgga gatggcacag gctatacaaa
    17941 tacaacagga aaagctaaag aattgataag tatagcaaaa agcaagctag gctgtaagta
    18001 tgtttgggga gctactggtc caaatacttt tgattgtagt ggattcactc aatggtgtta
    18061 caaaaagata ggtataaaca ttcctagagt atcaagaggc caaggaaaag caggtaaagc
    18121 agtaagtaaa ggaagtttac aaccaggaga tttagttttc ttttctagca aaggagcaaa
    18181 tggaacaata gaccatgttg gtatgtttat tggaaatgga gaatttattc attctccgca
    18241 tactggtgat gttgtaaaaa taagtaagtt aagtggtagc tattacacta aaaattatgt
    18301 aacagctaga agatatttat aaaaaggtgg tgatatagtg tctgatccaa taaatgaatt
    18361 tataggaata ataagagagg aaggaaaaca ttacaatgaa ccttcttttt ttgttggaaa
    18421 aattaaaagc aaattaccag atttaaaaat agagataaat aacatcgtat tagaaaaaga
    18481 agatattttg atagatagtt ggatgcttga tagacagata gaatcatttg atacagaaac
    18541 aaatcaagaa catcaacatg aagtaaaaaa tccatttata gatacttttg aatctggaga
    18601 catagtaata atgttcaaaa taggtgataa atttgctgtt gtaagtaagt tggtgagttt
    18661 ataatgagta caatatttcc ttttataggt gtcccagaag attatatctt gcctaaaacg
    18721 gaagaattgc caatctttcg tgaagtggcc tgggattttg aaaaagatga acctatttta
    18781 gaaaatggag attttaagat tattgaaggc aatgaagcta tcaaaatttg ggtgtataaa
    18841 tgcatcaaaa caaatagata tgagcatgaa atttatagct ggggctatgg aactgaatta
    18901 tctgaactta ttggacagaa atatagtaaa ggacttacag aaagtgaagc tagtagatat
    18961 ataaaagagg ctttattagt taatcaatat atattagatg tcaatatcaa ggatacaaaa
    19021 tttacagatg atttattaag tgtaaatata gaaatttcga cgatttatgg ggaggtagaa
    19081 gttaatgtat agtagtcaaa cttatgatgt cattaaaaat agaactctat caaatataga
    19141 tttagatatc tataagggtg agggttcttt tttaagtgat atggtatctc caatcagtac
    19201 agaactcgca aaattctata tagaactttc atatcttcat aaaaaagctt ttattgaaga
    19261 taattttgat gattttcttg ataaacgggt aaatgaattt ggagtataca gaaaactagg
    19321 aacagaagct acaggagaag tggtattcga gggaaaagct ggaacagtta taccaaatgg
    19381 aacaattata tcttacaatg agttattatt cgtagtaatt aaagatatag taattagttc
    19441 ggaaatcgaa caaaatacaa gccccgtaca ggctttagaa attggaatta gatataatat
    19501 acctgcaagt actgaattta agctacaaga cgaaataaac ggtataagta aaatatataa
    19561 taatttagac tttaaaggtg gtacagaaat agaaacagac gaagaattaa aagagagatt
    19621 ctataaaatc caaagaaatc aagctacaag tggaaataaa gctcactatg aagaatgggc
    19681 tttagaagtt gaaggagtat ataacgctaa aatctatccg agatgggatg gtccagggac
    19741 ggtaaaggta ttaatatttg gagaaaataa tcaagctgtt gacacagaag taattgaaag
    19801 atgcaaagaa catattgagg aagaaatgcc aataggtcct gcgttaaccg ttttaactcc
    19861 aagcgtttta gatataagta taagtgcatc tataaaatta gaagcgggat atacattaga
    19921 ttttgtaaaa gaaagcttct tagagagtat taatagttat ttaataaatg ttaataaaga
    19981 aataatttac actaaagtaa gtgcaatact tgcgtctatt gagggtatac atgactttag
    20041 caatttactg ttaaataaca aagctgaaaa tatagtattt gaagaagaca aagttccaag
    20101 tgttacgacc ctagagttta gtgaggtggt agtttaatga aattaattga taagctacct
    20161 tctttttaca acaataatgt tactagtaaa atacaagaag cttatgacat agaactagaa
    20221 acacttagag aaacttatga cgatactttt gaccagttct ttgtagatac agccacttgg
    20281 ggattggatt attgggaaaa tattttatct attaaaaata gatttgattt aaatatagaa
    20341 gatagaagaa gtaatataaa agcaaaaatg agaggtaagg gtacaactac gatagaagtt
    20401 ataaaagcta tatcagaagc ctacacaaag actaatgttg atgtagaagt ttttagcaat
    20461 ctatttagtt ttacacttag ttttataaca aatgattgta gttataacac tattttagag
    20521 ttagacaaga aaatagaaga aataaaacct gcgcatttag aacataaatt tgagagagtt
    20581 ttatttgata aaagtagact ttacgcaggt gcgacgatta gtgcagggga aacagttaca
    20641 atatatcctt atgtacctag agatttagaa agttttggag aaatagctat ttgcagtgga
    20701 aatgatagag cgctagaaaa agtaacattg tatcctaaaa aatagaaatg aggtgataaa
    20761 attggcagaa caacaatatt ttactctagt aactgacatt ggtaaggcag caatagcaaa
    20821 tgcaagtgtt acaggtgaaa aagtagattt tgcaaagata aaagttggag atggaggagg
    20881 gagttcttat actccaaatg agagtcaaac agcactaaaa aatgtagttt gggaaagcac
    20941 acttgaacat gcacaagtag ataaagacaa tcctaactgg gtagtaatac aaaaattcat
    21001 acctggcgat gttggaggat ttgaaataag agaagttggt ctattcgatt ccaaagacca
    21061 attattagcg gtttctagtt acccaacaac atataaacct gaagcagatt cggggactgt
    21121 aaaagaacta ttaataaaag taatattagt tgtatctaat gtagctaata taaatctaaa
    21181 agtagaccct actgtcattc tagcaacttt aaaagatttg caggacctag actctaaaat
    21241 tgatacaact aaaacagaat taacaaacag tatagaaact gctaaaacag agttaaacaa
    21301 caaaatagga gatacaacac aacttgaaac aacggataaa actagtcttg ttggtgcatt
    21361 aaatgaggta aaaactagtg tagatagcat agaaacaaca gcagagaaaa caagttataa
    21421 taatgcaaca agcaatctta ctgctacgaa tgtgcaaggg gcgattgatg aagttgttag
    21481 aaagatagaa aatttcaatg aggttaatat atctatacaa aatgatatgt tacctattta
    21541 agagaggaga gtgagaaaat gcagactgaa tgggatttta attatgctaa ttatgtacaa
    21601 aatgtttcat tgctacctgg gcgatataaa ttagaatgtt ggggtgcttg tggtggtact
    21661 cttggtgcaa gtgattggac tgaatgtgca aaaggcggtt attcaaaagg cgaagttata
    21721 ttaaaaaaaa gaactaactt atgtatctgt gttggtcaat cgggttatga aaaacttgat
    21781 actggttcaa ctcttaccag aactggattt aatgggggag gacgaacaaa tgcacatagt
    21841 attaatggca attattattc taaatacggt ggtggagcaa ccgatataag agcttattat
    21901 actagagcga cttgggatag tgcagaaggt ttactttcgc gtatacttgt tgcaggtggt
    21961 ggtggaggta tgaaaaataa ttttgcttct gctcgttcta ttggtcatgg tggtggttat
    22021 gtaggtgaaa atggaattgg tcgtgacaga gatttttgtg gtggtggttc tcaataccaa
    22081 ggtggaacaa gttacgatac agaagaatac catggttcgt tagggaaagg aggttatggt
    22141 ggaataggag taggtggagg tggtggttgg cacggtggtg ctggttctta ttctaatgaa
    22201 tgtggaggtg gtggaagtgg ttacgcacta actaaagata gttataagcc acctggatat
    22261 ataccaacat ctgaatattg gctagaaaat gtagttatga ctactggagg taatactact
    22321 agagcagatg gctacgctaa aataacatta ctacaagcat taccattttt aaatatatca
    22381 tcatataact caactactgc aacgtttaaa gctgaccata caaaccctac actactaact
    22441 aaaatagaat attttataga tgacacatta aaagaaacta taacaacaga tttaactctt
    22501 gaaaaaacaa ttaactatac attagaagat aacgccctac acacacttaa gatagttgtc
    22561 acagacagtg ctaatgctac agcagaaaaa aatgtaagta taagcaaagg aattgcacct
    22621 ctccctgctg gttctacaac agatgaagtt acaagcaaat ggatggaaat taaagataca
    22681 ttcaaaagtg gcaaaacaag tattataaat actttagcat taaagaatat agacgcaagt
    22741 ttaaataata cacttgttga gttatcagag aaaattaaaa cgagttttga tagttcagac
    22801 gctagtgttg aggatttaac aaacaaattg acacaagcta ataatactat atcacagtta
    22861 aatacaaaat ataaatttgc atatagtttg gatttgacag ttcactggtt tattgattgt
    22921 ggatttaagc ctgattttgt tatttatgaa tgtataaata atcatggtaa atttcttgtt
    22981 ttcttaactc cttttaatta ttctgtattt gatattggaa atttaggttg gacaattaca
    23041 gcaagagaag atataagcaa gaaagattct ttttctataa ataacataaa tcaattttgg
    23101 ggaacaaaga ttagagatac aggatttaat aatgcagata tgccaaaaga tagtttaaat
    23161 gatagatatg ttttttggat agcaattaaa tgtttatagg aggtttaata tatgaaaaat
    23221 ataggaagaa tagtttttta tgataatgat acaggggata tattaatgtt ttatggagaa
    23281 aaaacagtaa gtgacagtat agatattaat atccctaaga tagattctat tgagttgcca
    23341 tatggaagta tagattatac tagaaatagg ctaataggta taaatgtaga aacaaaagaa
    23401 ccaattctgg aagaaatacc agtatatgta agtgaagaag aaaaaagaat acaagaatta
    23461 gaaaatcaaa tattgattgc agaaaacgaa aaagtaggag gattattata atgaatataa
    23521 ataatgttgt agtaagaata ttagcagaga ggattttgaa taaaggatta aatcccttaa
    23581 aaaatagaga gtttcaactt gatgatgtaa ctaatgcaga gtacagaaaa gcagtagagg
    23641 attatattat agagcatagt ggagtagtag aaggagcaga accaacaaaa taggttcttt
    23701 ttttatttca attaattagg aggcttacat gaatgaggaa gttataaaag aaaaaataaa
    23761 acgaaatgaa ataagaataa acagacatag tgatgaaata gacgaattaa agatagcaaa
    23821 tatagagtct aaagcagaat taaaagcatt gtgtgagaat ctaaactcac ttacaagtat
    23881 gttaaagtgg ttgattggga caatgattac aacacttata gggttcttta tatttgcaat
    23941 acaaaaagga atattttaaa ttaggaggat aaaatatgga taatttaata agttttatac
    24001 cagagcagtt actaatttta gttgctgttc tttatgttat aggagcagga tgtaagaagt
    24061 ataaacagtt agataacaag tatattccag tagtgttatt agcacttggg ataggattct
    24121 ctatttggat gttaggatta aatcctagtg cagtcttaca aggtgtaatt tgttggggta
    24181 tatcaatagg tataaatcag acttacaaac agttgaggga ggagaatgaa taatggtaga
    24241 tataataaag atgttaacaa agaaaaagtg ttatccaaac aagaatagtc ctaaattcat
    24301 agtgattcat gagactgata atgaggacaa gggagcagat gctaagagac atgcacaagc
    24361 acttaataat ggtaatttag aagcaagtgt tcattactat gttgatgata aagtaatata
    24421 tcaaacatta gaccataaag atggagcgtg ggcagttggt aaaagctatg gaactgcatt
    24481 agttgcagga gttacaaatt acaatagtat taatatagaa atatgtgtaa atcgtgatgg
    24541 taattacaca aaagcaagac aaaatgcaat agatttaact agaaaactca tgaaagattt
    24601 aaatattagt acagataaag taataagaca ctatgatgct aagagaaaat attgtcctcg
    24661 taagatgctt gataatccaa aattatgggt agattttaaa gataagataa aaaatgatgg
    24721 gggtgtgaat agtatgataa aatacacaat agtctatgat ggagaggttg acagaatacc
    24781 agccgaggtt attggttgga attataagaa agaagaatgt agagtatgtg atataaaaga
    24841 atatatacct ggtcagacgc aaaatctgta tattgtaggt gggggagcat gtaataagat
    24901 aggttctata acaaaagaac attatacaat gataaagggt aatgataggt ttgaaacact
    24961 ttacaaagca ttagatttta ttaagaaata agtttgtaga gagtggtaac tatttcatca
    25021 gttaccactc tctctttttt ttatcctttt tgttatattc tctttcaagt gctgtttttt
    25081 taaaaaccca tgtcgttccg aattttttac agtctacacc ttccacaaat tttccgtttc
    25141 ttatattaag tcttaacgta ctttcacctt ttttataaag ttcacaagca tcttttatag
    25201 agattagttc ctcaaataca tctttcattt taacaactcc ttttacttat attataatcg
    25261 caaaagttta aaaagacaag gggaaaataa gcgtaaaagt ttaataaaag tgttgactca
    25321 ataaacgtaa acgcttataa tataattaaa gaaagaaata atcaaaagga ggatataaaa
    25381 aatgaataaa caaaaagcta gaagattttt aagagttata gatatgaata tagataaaat
    25441 agaagaagaa gctataaaag cttttaaaga aagttgttta atcaaagaga ctaatgatat
    25501 aaaaatttat atcgatatac aaggaaaagt tgaagcgata gcagttcaaa cttgggctaa
    25561 acttttaggt gatgacaaag aaattaatat tttcacatta aatcaagcac caactcattt
    25621 agacgatatg tttggagaaa tttaccatgt aaatgattat gaagagttcg aaaaatggtg
    25681 tgaaaaggaa gataaaagtt tgaattggga tagttatgaa aaattcaata aagaaaattt
    25741 cgaagaaatc gctgaaagaa atatagatga tagctcatca gcttttttag aaaaattaca
    25801 cgaaagcatt gaaaattgta aacaagaatt acaaaatata gttgaaaatt aagtagaaga
    25861 gtattattat taatgtatat agtatcgctt ttggttaact ataagggatt taaaatattt
    25921 tttaatttat ttagaaatga tattgtaata agaattttca gattaactat gagggattta
    25981 aatgtcaatt taatttatag agaaataata taatatcaaa ttattttata ttaactataa
    26041 gggatttaaa tatcaattta atctatatct atatgatata atatcacatt atttttagat
    26101 taactatacg gaaataaaag cagtgtattc aataaatgca ctgctttttt aatgctaaag
    26161 atttaattat tatgataact caatccttcg attaaaaatc ctacgttcat tgtcatttct
    26221 gcttcgtcgc tttgcaatat tccttccgta aattcgctgt cgcttggtaa tgatactttt
    26281 tttcttgcaa ctgcttctat gattgtttga aatagtttaa ttttcatatc cttatcaaat
    26341 ccagcgtatc gcaaaaaatt tacaacttcc tgtgaaaact tatttaattc agatttagtc
    26401 attttttcag ataactcttt tccaatttgc aaataaacat tcatcttttc caaccccttt
    26461 ttagattata gttaaaacat attatttaaa caatattgat atattgtaat aataatatta
    26521 aaatattact taaatattat atatacaata tttaagtaat atctaaacaa tataaatata
    26581 atattattta ctttttctta gtcttatttc ttctatttct ctcattgtat atatttaaat
    26641 tctcttcttt tatagtgaca tcagataaaa tttgagttag tatatcactt aataatttac
    26701 taacgtcgct gttcatatca atagaaaaag attttaattt gtttacaagt tcttcatcaa
    26761 gcgttgcact catagtcact tttttacttt tcttattaac taaattatat atgtttaatg
    26821 tcttattgtc tgtagttgta gaatcatttt gtgcgattgt tccatttttc aaaatcggtt
    26881 tttccttatc aactaaaagg tctctgttga aacttggcat ctataacact ccttttcttt
    26941 tcaattcttc taacatatta gtaaattcct gtctgctgaa atgttcttta gttatttttg
    27001 tataatctgt tagagatagt ttttttaata gtgccttctc tataaactca ttttttctaa
    27061 tgtatgtatc taacataata tctctcatat cgttaaaccc ttccagataa gaatcgaaag
    27121 tatcgctaat ttgagttttc ttcttctcaa atccaactaa aatagtactt tttatattat
    27181 cctccttatc aaaataggtt ctatcaacat cccagagttg tttaaataat tctgctcctc
    27241 ttaaagaaga aatgttttta tcttgaattg gaataattat actatctgct aagaaaagca
    27301 catttttagc tgtcaaatcg tagcgtggag ataaatcaca gataatataa tcatactcac
    27361 tcaaagtatt aaagttttgc atgtaccagc gagctaaaaa tttttctctg gctggtaaag
    27421 gtgatagctc ttgttcaaat ctagccattt gtatgtcaga aggaattaag tctacattaa
    27481 tgtaattttc attaggtgac ttgactattg catcattagc actaatccct tttaaaatat
    27541 ctaatgtagt attatcatta tgatttattt cataaacata ttgtgttaga tttgcttgta
    27601 catctaagtc ccataagagt atttttttat ctttatcttt cgcaagttca tacgctgtca
    27661 ttatagacgt tgttgtctta tagatacctc cttttatgtt aaagtacgta agtagctttg
    27721 tattagccat tttctccacc tcattattat atttataata ttataataat attgtagtat
    27781 tgtttttata atattgcaac aatatattaa tattgttaat tacaatattc aaataatata
    27841 aatacaatta aagtaaaaaa ttatctagac tttttgaatt tgatttttaa atcaaattcc
    27901 caaccactct tagtcgcgtt tgagtggttt tgtgttttta aactaattaa catatatgaa
    27961 gtgatgtaaa attgtatctt tttttaaaaa atgtgatata ataaaagcaa ggaaataatg
    28021 atttacctta cacaagagta gctatttcca atgctttgaa aaataactta ttttcttaaa
    28081 ccactcttag tcgcgtttga gtggtttttt attttatcat atatgaaagc tgatataaca
    28141 ccagctagta tactcaataa aaaatttatc accatactgt tccacctcct tccgttatgg
    28201 aatttggcgt ttaaatatgg aaatagccac ccttacgctt ttcaatcatt ttttccttgc
    28261 taaaattatt ataacatatt ttaacaacta actaataaac taattcatct ttaaacttca
    28321 tttcgctttt acaagcttta taaactgatg cgcaactctt gcaatcttct aatgctctat
    28381 gagaactata attcaatcct aattcttcgt gcaaagaagg caaagaataa ctttttagtt
    28441 ttttaccctc ataagttctt atgtactttc ttgacaaagt taatgtatct ataaccttat
    28501 tagtaatttt tttatatcca ttcttatata atttatctaa tataaattcc atgtcaaaaa
    28561 tagcgttatg agctataaga gtataatcac ttataaaatt caaaaagtgc ggaagcactt
    28621 cctcaatgct tggagcatta acaacgtctt cgtctgttat tcctgttata tcagttatct
    28681 tttttcttat actcttactt ggcttcacaa atgtactaaa aatttcgctt gggacatccc
    28741 tttcaaattt aatagctgct atctcgataa tatcttcttt gaaagcatct aaaccagttg
    28801 tctctacatc aaagacaata taatgctcta aaactttatt tttagaattt gtaaatttaa
    28861 taaatttttc gtactcaatt tctttttgac ttttaaaaga actaactatg ccactagaaa
    28921 gttctttgct aaaatcatat tgctttgcaa tatcaaattc agttagaact ggctctttta
    28981 atgtttttaa aaatcttttt actgaattaa acacaaagtc atcccccctt tacagtttaa
    29041 ttataaaaaa taagacttag tttgtatact aagtcttagt aaaatttaac attttaaaaa
    29101 aatatttcta aaatttaatt gattttttaa aacagtatat ttttttataa tctaaatgtg
    29161 ctataattat aatcataaaa tgagaataag ccgatttttt taaattagat ttttaaaata
    29221 caaaaaaata aaagaggaat actcacttgc cgtcaaacaa tagtattcct caccatcagg
    29281 gaaaatggac ccttattttg ggatacacaa atatccttat tttctaattg ctatattctt
    29341 attatagagt aaaaagcaat agaaatcaag tttttttaga gtatttttaa ttagtaaaat
    29401 tctactaaaa aacagaaaat aggttttatt tttccctgta aaataagggg gatttaaaat
    29461 gaaaaataat ataaataaat tatttctaga agaatgcaac gctgaaaggg cagaacatga
    29521 aaaagaaaat tattcaaatt tcaatagaca tgaaacatat gaaataaaaa aatatataaa
    29581 cgaaaaaaaa ttaggaatta aaacggctat agctaacgct ttggatggag gtaaaaccct
    29641 attcattgcg ccgacgggag caggaaaaag ttatagtttt ataaatacat taaaaaaatt
    29701 aaaaactaaa gcattattca tacttccaaa tgcttctaat gtagagcaag caatgcacga
    29761 atacaacata gctggtgcat atgataagat acctgctaaa caagctttag agaacaataa
    29821 tctagcagta atgacctggg acaagactga acagctcata gatgtagact taagagaata
    29881 cataatcgta gttgatgaaa tacatcaaac ttatacagac agctatagag ggaaagcaat
    29941 aaaaaattta aacaatatca tgtctaagtg taaaggaaga atagatataa cagcaacacc
    30001 aacaaaacta gaatttgaaa tatatgacta cataatagag tacactcaaa aacaaaaaac
    30061 agaatatgat gtcaagttat ataatgattt tgataataaa aattttacag aaataattaa
    30121 tataataaat aagtcaaaca atagtgccat gctaatgaat gatatatcaa ctcttgagtt
    30181 cataagagat tcagtaaaca aaaatgcggg agttgtatat gcagatggga aagaagaaaa
    30241 tgagttgtac agtagaatag ttaaaaattc agatatgaaa ggctacgaaa cgcttttaaa
    30301 tacaacaaca atcctagctg gtgtaaatat aaacaacaaa gatatcacag atataattat
    30361 tgttaatata aaagatattg gagctataaa gcaatatgtt gcaaggttta gaaatttaaa
    30421 aaaagtaaat gtacacatat tcaataaata caaagaagag tgtaatgtct ataaaataga
    30481 atggttggtt gataaaaata tagaaaaagc aactatctta aaagatgctt ataacaaagt
    30541 atctaaacat accctaatgt ttgaaacggt tgggataaat gctacaccaa ttcgaattga
    30601 ttcaaatgtt tattactgta tgaaagacaa ttgttacaaa gtagataaac tgtatataaa
    30661 aagccaaatt tacagtaact attacaacac aagaacaata caaagtttta aagttttatt
    30721 agaagaatac ttcgataatg ttaatataac tgatgctaaa gaaattgaaa caaatgaaaa
    30781 agaattaaaa gaatataaaa aagttgtaaa agaaattaaa gaagcaacaa gaaatatttt
    30841 aaaagaacat aaagaaattt tggtaggtta tagacagatt aaaagcaatt ctaagagctt
    30901 ttcactaatg cagtatcata atgatatgaa actaagttca aaagggtgct tagaggctta
    30961 cagagcgtat gacatacata atttggtaaa gaaaagtaaa agtaatagca tgttagaact
    31021 atattctaac tatgtgctag ataataaatt cagtttagac ctcgcttgga agttagcaaa
    31081 tacagcaaat agaaagcgtg gagcaatatt caacaagtta aatactttga tatacagaga
    31141 attaaaagag gaatatccag aatttttaaa tgatgaactt attcaagtta cagtatttaa
    31201 ttatatagac aaattatttg gaattggaac atcttataca gagttgcatt tacaagagtt
    31261 aagtgacgac ttaagattga ttttaggaga aaattggaac ttaacaacta aaaaactgag
    31321 tatcatattg aatgaagttt ttgtaataga aaagaaaaaa tatagaggtg ggaacagttt
    31381 agaaaatatc tttttttata aaaatataaa tcctaaactg ttcccactcg aagctgaacg
    31441 aatgcaagtt aataatataa aaagatatat aagcatagat gacataaaaa aagatttaaa
    31501 attagacgaa aaagacaaga gcctagaaca tgctataaaa catacaaaag atagaatgtt
    31561 gaactcttta gatgaagatg aaaaactgtt gctactaaaa ggttttatgt aaaaacatat
    31621 ggagggatga atatgaaatt aagaattggg attatagata aatatgtggc tgaggcaagt
    31681 tatgagttat cagaactttt aattgatgct tttaataaat atgatgaatt tgttgaggct
    31741 ataagaggtg gagaaattcg catagtccca tttaatttgg gatttcaagc tttgctggaa
    31801 gaggattcta cttatgatga gtatgaattt tattacgatg atgaggatga taaatgtttt
    31861 tttcatatta aatatagaaa tgttaaagat gttgaacttt caaaagaact tttgctagaa
    31921 cttataaaag ttaatttgcc cgcaggagca aaattaattg tgaacaacta gaaatttgtg
    31981 tttagataca aaagccggga gtaggaatat atatatatat aaattcctac tcccggcttt
    32041 ttgatatgag agctgtaggg tttaaatatt tgaccctaca gtttttaaat gtgagaaaat
    32101 tcaaagtaaa ttgaattggg attattttcc actagcaaaa tttgctaaaa ggtactatta
    32161 ttgtagtata aatgtttaat tacgacaaaa aatcgtacat gtgttcttgt gtaatcgaac
    32221 aaaaaagggt ataattaaag ttgcaagtta tttttttcaa attttaagaa tgtattattc
    32281 taataaaaaa tagggggatg gttttgttga aagaggaaat aaaaagaatg atagatttaa
    32341 ttggggacag agagttgtta gaaattattt atttatatct aagaaaaaaa actaaaaata
    32401 aatgagtaca tataaaaaag ctatcaaaaa tgagatgata gcttttattc tttagaaaat
    32461 acttctaaaa ttttcaaaat tgattcgagt tgctcgtctg ttaatttgct aaattctaat
    32521 actgctttcg ctagtttttc gttttcgtct attgcgcaaa aagcctccgc tttcacggag
    32581 tttctatcct cagtgttata catctctcct gtgccagttc ttagccaatt ctcatttacc
    32641 ttaaatagtg aaattattaa ttttaaatca ctttccttta ccttcgtcct tccactttct
    32701 aaattgtaaa tctcatgctt tgtctttcct aatttttctc caaattctat ttgctttaac
    32761 ccctctgatg accttaactt tctgattcgt atatttatat cgtctattat ttcgttcatt
    32821 tacaaaactc ctttcataaa ttgattttac cttaaaatat tatttaagtc aacgtaatta
    32881 tacgtccaaa tggtaaaatg aaggcataat aaatttcaat atagtattga cagtactcct
    32941 ataacgtcat ataatgtatc tatagacgtt ataggagtac tgggaggtgt ttttatgaat
    33001 agagaagaaa tgatttgtaa aagtgtgaaa ttatatttaa gtttaagcg
//
LOCUS       DAFKMX010000019        32071 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFKMX010000019 DAFKMX010000000
VERSION     DAFKMX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.019"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1000
                     /locus_tag="LZB08_000786"
     CDS             <1..1000
                     /locus_tag="LZB08_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBM7314301.1"
                     /translation="KNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTEEICL
                     EAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTALKYIE
                     NQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEAVKHC
                     SYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQTVGI
                     CLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPFAKRY
                     VRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLGVF"
     gene            1136..1624
                     /locus_tag="LZB08_000787"
     CDS             1136..1624
                     /locus_tag="LZB08_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7314302.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1800..3728
                     /locus_tag="LZB08_000788"
     CDS             1800..3728
                     /locus_tag="LZB08_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7314303.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            3905..4315
                     /locus_tag="LZB08_000789"
     CDS             3905..4315
                     /locus_tag="LZB08_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBM7314304.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4337..4639
                     /locus_tag="LZB08_000790"
     CDS             4337..4639
                     /locus_tag="LZB08_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBM7314305.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(4856..5548)
                     /locus_tag="LZB08_000791"
     CDS             complement(4856..5548)
                     /locus_tag="LZB08_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBM7314306.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCREGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5574..6761)
                     /locus_tag="LZB08_000792"
     CDS             complement(5574..6761)
                     /locus_tag="LZB08_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7314307.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPKKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7097..8974
                     /locus_tag="LZB08_000793"
     CDS             7097..8974
                     /locus_tag="LZB08_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7314308.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9331..10737
                     /locus_tag="LZB08_000794"
     CDS             9331..10737
                     /locus_tag="LZB08_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBM7314309.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10765..11613
                     /locus_tag="LZB08_000795"
     CDS             10765..11613
                     /locus_tag="LZB08_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7314310.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12274..12966
                     /locus_tag="LZB08_000796"
     CDS             12274..12966
                     /locus_tag="LZB08_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314311.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            12963..13898
                     /locus_tag="LZB08_000797"
     CDS             12963..13898
                     /locus_tag="LZB08_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314312.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14046..14969
                     /locus_tag="LZB08_000798"
     CDS             14046..14969
                     /locus_tag="LZB08_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314313.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            14953..15723
                     /locus_tag="LZB08_000799"
     CDS             14953..15723
                     /locus_tag="LZB08_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314314.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15733..16515
                     /locus_tag="LZB08_000800"
     CDS             15733..16515
                     /locus_tag="LZB08_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314315.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            16892..17428
                     /locus_tag="LZB08_000801"
     CDS             16892..17428
                     /locus_tag="LZB08_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBM7314316.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17460..17849
                     /locus_tag="LZB08_000802"
     CDS             17460..17849
                     /locus_tag="LZB08_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBM7314317.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            17886..18272
                     /locus_tag="LZB08_000803"
     CDS             17886..18272
                     /locus_tag="LZB08_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBM7314318.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18399..19841
                     /locus_tag="LZB08_000804"
     CDS             18399..19841
                     /locus_tag="LZB08_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBM7314319.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20138..20953
                     /locus_tag="LZB08_000805"
     CDS             20138..20953
                     /locus_tag="LZB08_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7314320.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIHSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            21555..22739
                     /locus_tag="LZB08_000806"
     CDS             21555..22739
                     /locus_tag="LZB08_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314321.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(23116..24225)
                     /locus_tag="LZB08_000807"
     CDS             complement(23116..24225)
                     /locus_tag="LZB08_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7314322.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            24688..25881
                     /gene="nifV"
                     /locus_tag="LZB08_000808"
     CDS             24688..25881
                     /gene="nifV"
                     /locus_tag="LZB08_000808"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBM7314323.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            25906..27831
                     /locus_tag="LZB08_000809"
     CDS             25906..27831
                     /locus_tag="LZB08_000809"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBM7314324.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            27853..28848
                     /locus_tag="LZB08_000810"
     CDS             27853..28848
                     /locus_tag="LZB08_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBM7314325.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            29174..29647
                     /locus_tag="LZB08_000811"
     CDS             29174..29647
                     /locus_tag="LZB08_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7314326.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            29681..30175
                     /locus_tag="LZB08_000812"
     CDS             29681..30175
                     /locus_tag="LZB08_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7314327.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNQNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            30272..30793
                     /locus_tag="LZB08_000813"
     CDS             30272..30793
                     /locus_tag="LZB08_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7314328.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            30982..31293
                     /locus_tag="LZB08_000814"
     CDS             30982..31293
                     /locus_tag="LZB08_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBM7314329.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            31321..32007
                     /locus_tag="LZB08_000815"
     CDS             31321..32007
                     /locus_tag="LZB08_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7314330.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKGKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT        11998 a         3652 c         5846 g        10575 t
ORIGIN      
        1 taaaaatgat atatttgaat caatagatac tgaggatttt gataataaaa cagaagaaga
       61 atgtttagag ctagtaagaa aaaatggatt gaatttaata tacataaaaa atcaaacaga
      121 agagatatgt ctagaggctg ttagacaaaa ttgtaatgca ataaaatgtg ttcagaatca
      181 gacagaaaaa atatgtatag aagcagttaa acaagattgg agaatgttag aatttgtaga
      241 agagccaaca gaagaaattt gcatgttggc tattaaccaa gatggaacag cattaaaata
      301 tatagaaaat cagacagagg agttatgctt aagagccgtt gaaaaaaatg gtgcagcttt
      361 ggagtatgta aaagaacaaa cagaagaaat atgtatagaa gcagttagaa acagcgaatt
      421 tggattggca agagtgaaaa atcaaacaga aaaaatatgt atggaagcag ttaagcattg
      481 tagctataat ctaaaatggg tagaaaatca aacagaagaa atatgtatgg aagcagtgag
      541 acaaaatggg cttgatttga aatttgtaaa aaatcaaaca gaaacaatct gtttaagagc
      601 agtaagacaa aatggaatgg cattagaatt tgtaaaggaa cagactgttg gtatatgctt
      661 aaaggcagta agacaatatg gaatggcatt aaaatttgta aaagaacaaa cagaagaaat
      721 ttgtacagaa gctataaaac aagataaaag agcacttagt tttgtaaaag gagataagga
      781 aaaatacaag gctctatatg ataataatga accatttgca aagagatatg ttagaaatgt
      841 aatagaaaaa gaaaacagag cattgattaa aaaaggagaa gaaaatgcaa aaatcaaaat
      901 tgcaggaaaa ctttatgata gaggcatgtc ttttgaagat atatcagata tagttgagat
      961 tgaaataagc aagttaaaag tgagtttagg tgttttttaa agtaataaat gtaggtgttt
     1021 tttatgggaa atagttaata aaaaaataat cgactagctc tttgtaggac agcaaaatta
     1081 gtgatataat ttgatgtata ataaattatt tttattagat aagggggaat taaagttgaa
     1141 gattacagta ttataccaaa caagaagtgg aaacacagaa agggtcgcta agctgataga
     1201 agagggcgct aaaaaggtgg atggagtaga tgtaaagctt atgagacttg ataatattga
     1261 tttagattat ttaaatgaaa gtaaagcagt aatatttgga acacctactt atttagcaaa
     1321 tacaacttgg gaagtaaaaa aatggtttga tgaagattca aaaaaagtta atttagctgg
     1381 aaaattagga gctgtttttg ctacatgtga ttatatatgt ggaggtccag atgttgcgat
     1441 acttactata gtaggacatt tgatggttaa agggatgctt gtgtattctg gaggtggttc
     1501 tttaggaaag ccatttatac atttaggaca tgtacattca attgaaggtc cagagttaca
     1561 agatgagaag gctataatat ttggtgaaag aattgctact aaggcaaaag aactatttgc
     1621 ttaatttcta tatgcatagt aatgattaga gtaaatttag ggttttaatg gagatttgaa
     1681 gtaaaagtta taaaataaaa aattttccat aaaattactc aagatgaatt ataatataat
     1741 tataaataaa ttatattatt aaaatattta aaatcaaacg tcaatagcga gggaagttta
     1801 tggtatggca tatagaatat gagatttttt ctgcaattat agttattttt ttaatggtat
     1861 acttttttag aagtaaattt atacctactt tacaaaacaa aatatattgt gcacttttaa
     1921 tattctcatt tttatttata atttcaaata ttttaggttc attttgtcta aataatatag
     1981 acaaaattcc aatttttatt acttttttat tgaatcagat atacttattg ttattgccaa
     2041 taccagcagc acttgtatca ttttatgtta tggctattat ttatcaagat ataaggtata
     2101 tgaaaaaaag attattattt ttatctatac cacttataat ttgtataatc ttatctatta
     2161 caaatccttt tacgcatatt ctatttggat tgtcaaaaga aacaggttat atacgaggtt
     2221 gtggatatgt tgcaactttt gcatcttttt tcttttatgc aatatattca gcatttttat
     2281 cttttaaata tagaaaagca atgcatgaat caaaaatatg ggctattaga ggttttctta
     2341 tagtttcaat tgtggcaatt atcttacagg caattttttt acagtatctt cttacaggaa
     2401 ctgcatgtgc atgtagttta cttttgacat atctttctat gcagaatcga ggtcttatta
     2461 ttgatgacct tactggtgta ctcaatagac aatcttttat tcaagagcta gatttgaata
     2521 tcaatactga acaatcagga tttattatta caattgcgct tgatgatttt aagtttatga
     2581 atgaaacttt tggaacaaaa aatggagata ttgcactaaa agaagttgga aaatatttga
     2641 ttcagataca tgatgtaaac catgtatata gaataggggg agatatattt tctattgttg
     2701 tcaatagtca attaggtata gaaccagatg atattatcag aaagatagaa gaaagattta
     2761 aaaagccttg gctagtagaa gagataagtt ttaatttagc aactagtatt gctgttgttt
     2821 attacccaga aaatgcagaa acgacagaag atgtaattgt tgctatagat ttctctatac
     2881 atgaagcaaa acgttctgag tcaaggcgag ttgtttatgc agatgcgtct ataagtgaaa
     2941 agataaagaa gaaacatgtt attaaaaatt gtttaaaaga agcattaaaa aatgatgggt
     3001 ttgaagtgta ctaccaacct atattttcta atagcagggg caggtttact tcagcagagg
     3061 cattacttag gctagaacac aaagagttag gttttatacc tcctgatgaa tttattccaa
     3121 ttgcagaaca aacaggattg attaactcaa ttggattagt agtctttgag aaggtatgta
     3181 gatttatagc tagtgatgag tttgaagatt taaaattgga taatatagct gtgaatcttt
     3241 ctgtagtaca atgtatgcaa aaaaacttag ctgatgattt actttatctt atgaataaat
     3301 ataatgtatc accaactaaa tttaaacttg aaattactga gactgtggct atgggctctt
     3361 ttaatataat taaagaaaca atggaaagat taattgattt aggtgttaag tttgcacttg
     3421 atgattttgg aattgcttat tctggagtta ctaatatgct ttctttacca ttttcagtaa
     3481 ttaagttaga taagagtttg atttggtcta tgaatgaaga tagtaggcat aaacttacag
     3541 ttgagacgat tatagcactt ataaataaat tgaatatgaa ggctgtagca gaaggtgttg
     3601 aaaccattaa gtgtgctgaa gatttaatag ctatggagtg tgaatatttg caagggtatt
     3661 atttttctaa accagttcca gaagatgttt ttaaaaattt gttgaaagaa aatactttta
     3721 caagttaaca ccctttattt ttttaattac aactttgttc attgacactg ccagcgatat
     3781 attataaaat taacacaata ataaatatta aatttattat acatattatt aatattaatt
     3841 aatggagtaa agatttatta tatattttat ttaattatcc taaagtgaga aaggaagatg
     3901 tgacatgaat aaaaagaaaa tattagtgcc aattgatggg acagaaagaa gtatgcattc
     3961 tttggaattt ataaaaggaa tatttaaaaa agatgaagtt gaagtagaaa taatgaatgt
     4021 aaaagaacta gtatttattg atggcatatc cctggctgaa gagataaaaa attctgaaaa
     4081 tttaggaaga agaatcttgg ataaagcagc agagataatg ggagagtatg atgttaaagt
     4141 acattttact tttggatatc caggagatga gataattaga aaagcaaaag aagataatgt
     4201 agattttata gttatgacaa aatctactaa aaaaggtctt acaagaatga taggttcagt
     4261 aacagcaagt gtagttaaac aagcaaaatg tatagtgatg attgtaccgg agtagaatta
     4321 gaaaggtatt tgaaatatga ataaaaagat agtagcagtt tgtgaaagtg aaagaagttt
     4381 aaattctctt aagacagctg caaatgattt aggatttgat ttagtttatg aaattcaaga
     4441 aaatgataat ataaaaaatg aactttctat ttatgatata gaagatgcat ccatagtgtt
     4501 atttgttaca agtgattcta tagaaagcat agagaagatt gatagattta tagatagaga
     4561 attttatgaa gttgatccaa agtatataat agaggatgca aaatctatag taagtgaaat
     4621 tttaatagac ttaaattaat cgtgggtgtc gcactaagaa tagagcgacg cctttttata
     4681 tattttacac aagaaataaa gaggttgtat caaaatatag ataagagtct ttttcattgt
     4741 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tacctcataa ctaaaaagtt
     4801 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata taagtttaat
     4861 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
     4921 aagagtcttt actatcaaat gcaatcatta tcttgttatg gtggctaatc atatccttga
     4981 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
     5041 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgca
     5101 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
     5161 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
     5221 gtttagtagc ttcgctttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
     5281 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
     5341 caactatagg acttgaataa ggcacagctc taagtattcc ttccctacag agtatattta
     5401 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
     5461 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
     5521 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
     5581 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
     5641 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
     5701 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     5761 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     5821 taagaaagga gtatacctgt agatatacct ggtagtactt gtagtacaaa tattatatta
     5881 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     5941 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     6001 acagcacaca ccatgtaaaa tatagaagat agacctatga gggtagaact agaacctaag
     6061 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     6121 aatgccataa aggaaaaaaa tacaagtctt ttatccttgc agatagataa atatttttta
     6181 attgatattt ttttaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     6241 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     6301 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     6361 gtacttgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     6421 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     6481 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     6541 aatattttat gtttatttat agaatcagca aatactccaa caggaagcct taataaacat
     6601 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     6661 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     6721 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc cgtatggtat
     6781 ttttataatg caaataatat accatttaga ttttttaatc aagtacttta tctattaaaa
     6841 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     6901 aacgcttatg atactataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     6961 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     7021 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     7081 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     7141 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     7201 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     7261 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaaaag tatatgatga
     7321 ggttataaaa attgtacctc aatcaaataa cataggtaaa aagaatgaaa gtcaagaaaa
     7381 gcaaggtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     7441 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     7501 gaataaaaat ggagtagata taaaagaaac gcaaagctat attatcttaa atgctatggc
     7561 tgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     7621 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     7681 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     7741 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     7801 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     7861 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     7921 cataaacttt tcttataagt atatttatga attaagccca gcattgtgtg gagcatttgt
     7981 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     8041 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     8101 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     8161 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     8221 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     8281 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     8341 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     8401 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     8461 agagtcttct aataaggaaa attcatctat aaaagtagaa acgaaggatg gtgttattga
     8521 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     8581 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     8641 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     8701 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     8761 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     8821 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     8881 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     8941 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     9001 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     9061 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     9121 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     9181 agtaagttta ttgtaatgaa ttgttaaaga aaataattaa tatagggtaa atctatctca
     9241 atgaaataac aaagaaaaga gttaatatag agtgaactta ttgcaatgaa tcaacaaaga
     9301 aaataatcaa tataaaaagg agaaatattt atgaatacag gatttccaaa agacttttta
     9361 tggggagcat catcttctgc atttcaagtg gaaggagctt gggataaaga caataaagga
     9421 aaaaccgtag ccgactataa ttcttttaaa aagtctcatt tgcaggcaga tactaaagtc
     9481 gcatcagact tttaccataa ttatgaagaa gatattgaat taatgaaaga attagggatg
     9541 aaaacatatc gtttttcaat ttcatgggca agaataatac cagatggtga aggagagata
     9601 aaccagaaag gtttagactt ttataataag ataatagaca aactaataga gtgtgatatt
     9661 gagccattcg taactttata ccattttgac cttccattta aattagtaga gaaatataat
     9721 ggttgggaaa gtagagaaac tgtctatgct tttgaaagat ttgctaaaat atgctttaag
     9781 cattttggag atagggtaaa gtattggcaa cctcataatg aacagaattt aattgttaga
     9841 gtggaggaga gaattaatat atatgatgaa acagattcat ggaagataga taaaatgcgt
     9901 gctcagatgg attataatct ttgcttagca catgcattag cagtcaatgc atgtcatgag
     9961 atgataaaag aaagtaaaat tggagcagca gtatcatcat ctgtaacata tcctcttact
    10021 tctaagccag aagatgtata tgcagctaga atgaatgata attttaaagt gtactatatg
    10081 cttgatatgc atcattatgg agaatatcca gggtattata tgaagtatct tgaaaaaaga
    10141 aatatagttc ctcacatgga agatggggat aaagagatac ttaaaaaagc taaaatggat
    10201 tttatagctg taaattatta taggacaaat tgtgcagaag ctttacctga agattcacaa
    10261 catccatttg gattaagaga aggaactgtt gattttagta tgtatggact atttaaaatg
    10321 tcaatgaatc ctaacttaga ggcttctgag tatggagcag caatagaccc atcaggtctt
    10381 agagttgcct taaatgaata ttggcaaaga tatcacttac ctgttataat aacagaaaat
    10441 ggtctaggag ctaaagatat attagaagat ggaaaaattc atgatgacta tcgtatagac
    10501 tatttaagaa gtcatataaa tgcatgtaaa ctagctattg aagatggtgt agagatgatt
    10561 ggatattgtc catggtcatt tacagacctt ttaagttctt ctcaaggttt taataagcgt
    10621 tatggacttg tttatataaa tagaactgac catgaagttt tagatttaaa aagaatcaag
    10681 aaagatagtt tttactggta taaagaagtt atagagaaca atggaatagt aaaataaaca
    10741 taaatatata ttgagggttt tagtatgtac attacaaagg tattaaataa ttcgttgcta
    10801 cttgcaaagg atgataatga tgaagaaata atattgatgg ggaaaggcat tggccataat
    10861 tataaggccg ggtctgagtt aaataaaaaa gatatagaaa agatttttgt tcttcatgat
    10921 gaaaatataa aaaagagtat tatacaattg gcaacagaaa tagatgcaga atattttggt
    10981 atagctcaaa tgataattgc atatggaata gaaaaatata acttaaaact tatgaaccat
    11041 atatatttgg ccttaactga ccacattgcc tttgcagtga gacgttttaa agaaggtata
    11101 atgattgaaa atcactattt gttcgaaatc aaggaattta atccaaaaga gtatgatata
    11161 ggaaaatatg ctattactgt ttttaaggag gttcttggtt tagacctacc agaagaagag
    11221 attggtaata tagcaggcca ttttataaat gcacaacaag ataatcctta tagtgataga
    11281 aataagagaa gtgctaaaat tgtaaatgct attttgcaaa tagttcatta tcacttttct
    11341 attgtatata ataaagagtc attttattat aggagatttg taatgcatct aaaggccttt
    11401 tcacaaagat ttctggctaa tgaaccatca agagaaaaaa tagattttat atatgagcaa
    11461 gttcaaaaaa attgtaagga agagtatgaa tgtgtgaaaa aggtaggagc ttatatgtat
    11521 aaggaatact ccagagaatt accaagacaa gaggagttat atttgatgat tcatattcat
    11581 aagattttag gagaattatg cgaaaatagt tagaatatct taatatgcta gtaatattat
    11641 aaaaaattaa aaattaactg cactttaaat atgaataatt gcattactta agggggttgt
    11701 cttaaaattg atttttttaa ttaaatttga agctcaactc cttttttaaa cctttatatc
    11761 attacaactt taatgtttat acgactgtac tttagatgta tatctacttt ttacatttct
    11821 caacatgcaa acctaccatt ttttgcagta tgttaattga aataaaccat tgagttaacc
    11881 ctaaatttat tctggactta taggaatagt tccaaagata ctttaaaata ttttctggaa
    11941 taattgctta tttcacatat gttttattgt gatgttttga tttaataaat tcttcaactc
    12001 tgtatatagt aaatgtatta tcatgtttgt tttattcaag cacacttata atacgcttga
    12061 ttttttcaca atgatgaaat atactaatgg gtgttatagg tataagaata gcattacact
    12121 tgaagaagta agtgataaat gagatataaa tcctcatatg ataaactact atttcattga
    12181 ggaacatatt ctaagtgatg tcaagatact acaatatgac taaattccta gtgataaaaa
    12241 agtctacgaa tgagtgtaga aaggggaact aagatgaata aagttttaat tatagatgat
    12301 gataaagatc tttgtatttt gttaaaaaag agtatttcaa ttgaacagat tgaagcaaac
    12361 tattgccatt ctgggagaga tggactggtt gaactttcaa aacaagatta tcagttagta
    12421 gttctagatg tgatgatgcc aggtatggat ggttttgaaa ctatggaaca aatacgaaaa
    12481 tatagcagta ttcccatctt aatgctgaca tcaaaaagcg ataattacag caaagtgcat
    12541 ggattgcgag ctggtgcaga tgactatcta accaaaccat ttgaaattac agaatttact
    12601 gctcgtgtac tgtctttaat ccgacgttac acccgtttta atccaaaaaa tgaggagcca
    12661 cagcaacttt ctttcaaagg tttgttgatt gaccttgacc agcatacagt gactaccaat
    12721 ggcgaaccta tgttgcttcc acccaaggaa tttggtgtgc ttctttactg tgcaaagcat
    12781 cagggtaaga ttttgacaaa acagcagatt tatgaagccg tgtggggtga gccatatgta
    12841 tatgatgaca gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat tgaactagac
    12901 ccatcatctc ccatctattt gcaaacaata aaaggaattg ggtatcaatt caacacggag
    12961 gtatgaaaat ggacgaacaa acgataattc ttttcttact cttgctgtcg attggtctac
    13021 tttttctttc catatttgct ttttccaagc ttgggcatat ttacaagcgc ctaaaagata
    13081 tagaggagat actggctgat gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc
    13141 catgtgatgt tatggctcca ttggtttatc agcttaatga gattgtctac gattatgaaa
    13201 ataagttact ttctctgaaa aagtcggata aagctagcaa acaacttatg acaagtttat
    13261 ctcatgatgt tcgtacacca ttaacaactc tgattggtta tctggatgct gtacacagtg
    13321 gtatagttat cgaacaagaa cgagaggaat accttgaaat cgctaggcgt agagcctacg
    13381 acctcaagga ttacattgat gttctttttg actggtttcg gttgaactct gatgaattta
    13441 cgctgtcaat agaatctgta gaaattgcag agttgtcacg caatattttg aaagactgga
    13501 ttcctatatt ccatgaaaag aaactgaatt ttgaaattga tattccagaa aatcgactaa
    13561 tggtaaacct cgacccagat ggatattccc gtgtggtcaa taatctggtt caaaatgttc
    13621 ttgctcacag taaggctcgg caaatcaaga ttaccatgag tgaggattcc agaatggtat
    13681 tactccgtgt tgaggatgat ggtgttggga ttgccagaga aaatttacca catatttttg
    13741 agcgactgta taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta
    13801 ttgtcagtca aatggtggag cgaatgggtg gtcaggtttc cgtagaaagt gagattggaa
    13861 agtataccgt atttacagtg agcttaccat taatataaaa ttgtttttcc cccatgtgac
    13921 atgtatacac agggggattt tttgtaagtt ataaagttgc aaggttaata caaggttgtt
    13981 gcaaggttaa tgtaagattg aggtggtatg ataactttaa taaacaaagg aggcttttat
    14041 tatttatgaa taatttaatt attgaaacaa aaaatctcac taagcaatat ggtgctcaaa
    14101 agagcgtttc taatttgaat attcacgttc agaaaggtcg catttacgga ttgctaggaa
    14161 gaaacggtgc gggtaagacg acaactatga aaatgttatt gggtctaaca aaaccaacta
    14221 ctggagaaat taaaatattt ggtcaggaca ttcacaataa cgagaagaag cttcttcctc
    14281 gaattggatg tttgattgaa tcaccagggt tttatcctaa tctgacagga acagaaaatc
    14341 tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg
    14401 atgtagtcgg tttgccaaac aatgacaaaa agttattttc tcaatactct cttggtatga
    14461 aacagaggtt ggcgattgct cttgccatta tgcatgaccc acaactgttg attttggacg
    14521 agcctatcaa tggacttgat cctattggta ttgcagaagt aagaacgttt attcgtgatc
    14581 tctgcacaga acgaggtaaa actattttga tttccagtca cattctttct gaaattgcct
    14641 tgttggcaga tgacattggc attattgacc atggtatttt gttggaggaa gaaagtcttg
    14701 cccaattaga agaaaaaaac agtaaataca tacattttgc tgtatctgat gcagcacagg
    14761 cagcaaggct tctggaaacc caacttaacc tgaaaaattt caaagtagca gataattaca
    14821 atattatgct atatgataca gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa
    14881 acaacattga ggtatcggaa tctcatttat gcgaagatac tcttgaagat tacttcaaga
    14941 aagtaacagg gggtgaagga attgcttgat ataatttcat gtgaattttc aaaactcaag
    15001 cggttaaaat ttatactgat ttctatttta ggtgcttgct tatttccaat tcccgcaaca
    15061 attttgattg caaaggacaa cttgcccttt gagcaattgt tcaaattagt tgtcaatttt
    15121 ggatattttt tgctattacc aattgtatta agtattgttg ctagccaact attttttata
    15181 gaaagagata atgatgtatt aaaaaatctt gcaacagtgc cagtgcaaaa aggaaaactg
    15241 gctctggcaa aattggctgt gttgctgttt atatcattgt tctactctgt tgcaggatta
    15301 ggagctacta tcattggagg tttcattgtt ggaatagtag agggggttgc tgtaaaatta
    15361 ggaatgagca ttgcttttgg aattatgttt tttgttactg tacttcctgt tgtggtactc
    15421 attgtatatt tcaatcgcag ctatattttt tctattatac tttcttttgt atatgctatt
    15481 ttcaatttta gtatatcctt aaatattatc aattttgaac caaataaccc aatattaagt
    15541 gtattacctg ctcctgttat aatgcgttgg tggatggcat tttggggaga tcccactggc
    15601 gaatacacgg cacttagaca gccatatttg ctctcaactc ctgcttgtgt aggtatttta
    15661 tgtttgataa ccattatagc agtcctgctg atttgtatga tatacaaaaa gcaggagaat
    15721 taggaggtat ctatgcttga tataattact acagaatttc aaaaaataaa aagatataat
    15781 attctatgga ttggtgttgt tgctgttctg ttttcagcac tattggcagt ttttcaggtt
    15841 gtaagttcac acggcagtga ccctctaacg tatgagagct ttgcaaacgg agttatctgg
    15901 aataatttca gtcttagttt tccatttggg attacactta ttggaggata tcttattaac
    15961 cgtgaatata cagaccaaac cttaaaaaac atcctaactg ttcctatttc attacgcaaa
    16021 ttgctagtgg gaaaattaat tgcggttgga ggaattactg tattgttaag tcttttcagc
    16081 ttcttatgca catttatttt ggggttgttg ttttgcaata tcaatatatc tgttacactc
    16141 gttgtaaaat ccttagttca gattttgtct gttaatctgt attgttatgt tgctgttttg
    16201 ccgattattg cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt agggattgca
    16261 tttgtatacg gtttttgtgg tgtgtttgtt gctggaagaa atctgacaga cttttatcca
    16321 atcacagcag gtcttggtct agttggatac aacaatggtg ctggtactgt ttatcagcca
    16381 cttatcggtg ctatgacttt gattgtaatt ctaatcttga caactattct acttgtattt
    16441 acacctaatt atgataaagt tatgaccata tctaagaaaa aagggataaa gcataaaaag
    16501 tattctcata aataacataa atattgtttc tattatttaa gttgctagac agtgaaaaga
    16561 aaaaatctgt cgtacagtgt ctatatgctc atgtattcaa gaagctacag ttttgatttt
    16621 tcataactgt agcttctttt tgtatttatt aaatcacgac tatcataata cttgcaatag
    16681 gtatagcgaa aagagagagt actgtcatat gtgttttata tattacatta agtatattct
    16741 aaaatatatg ttttaaataa tatatttgaa aataggaata tttaaataaa aaaaataata
    16801 ttatatatag ttgacaaaaa ttggcaaatg agataatata taaatataga aatagtaatc
    16861 attactatta ttaaaaaggg aggaattaat tatgaactta aaaggaacta aaacagaaaa
    16921 gaatttaatg gcaggatttg caggagagtc agaagcaaga aataaatata catactacgc
    16981 ttcaaaagca agaaaagaag gttataatca aatagctgct atatttgaag aaactgctaa
    17041 caatgaaaag gaacatgcta aattatggtt taaactttta catgatggta tgccttcaac
    17101 agaggaaaat ttaaaggatg cagcagcagg tgaaaactat gaatggactg atatgtatgc
    17161 aaaatttgct aaagaagcta gagaagaagg atttgacaag atagcttatt tatttgaagc
    17221 tgttggaaaa atagagaaag agcatgaaga aagatactta aagcttttag aaaacttaaa
    17281 tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg caatgtcaaa actgtggtca
    17341 tgtgtatgta ggtactgaag caccagaaaa atgcccagta tgtgatcatc caaaagcgta
    17401 tttcaatata aaagctgaaa attattaaaa attatcatta ttaaaaagga atgttaacta
    17461 tgaaattttc taaacaacga gaactgattt taaatgaaat attaaataat ccggttcatc
    17521 ctactgcgga ttacctatat gaaaacttaa aaaaagataa tccaaattta agcttaggaa
    17581 ctgtgtatag aaatttagct cagctaacgg aacatggctt tataagaaag gttagtattc
    17641 cagggtatcc agatagattt gatggtcgaa tagataatca ctatcatatc atatgtgaag
    17701 tatgtggaga ggtatacgat ttagaatccg aagttcttaa taatttacaa gaattaatat
    17761 ctgaggagac agacataaaa ataacatctt ataacataag ttttaaaggg atttgtaata
    17821 attgtaaaag gtgtagccaa gtaggttagt tttcaaattc taaaaataat taatgaggtg
    17881 agtttatgtg tagtgaacaa aaatttttta tatgtgagac ttgtggaaac cttgtaggaa
    17941 tgatacaaag tggtggtgta cctattttct gttgtggaaa acctatgaag gaacttgttc
    18001 ctaatacaac agatgcagca gttgaaaaac atgtacctgt aattgaagtg gatggaaata
    18061 atgttactgt taaagtaagt agcacaactc atccaatgac taaagaacat catatagcat
    18121 gggtgtatct tatgactgaa caaggtggtc aacgcaaatg tctagcagta gatggagaac
    18181 ctgtagttaa atttgctttg aatgatgatg ataaagtgat ttctgcctat gcttactgta
    18241 atttacatgg attatggaag gctgaactgt aatatttaaa taaattgagt caaaaaggag
    18301 gctataaatg tattttcata caaatagcct cctataaatt tatataaatt caagttttaa
    18361 ttttgtttaa tgtcaatata atataaggag aaaataatat gtcaatttat aaatgtagtg
    18421 tttgtggata tatttatgat gaatctaaga atgataaaac atgggatgaa ttaagtgaag
    18481 attgggaatg ccctgtatgt acaaaaggtc gtagttattt tggaaagata agtactgttt
    18541 actatgaaga agatgaaaag atagcagaag acatagttga agatgaatct aaattaaata
    18601 ctgaaaaaga gggagattta aattacttaa gtacatattt aagaagagat gatgaagttg
    18661 agaaacatat ggatataatt cacgaaatgg ctgttacagg aaaatctata atagaaccta
    18721 tgagaactaa actaccagta atttcttggg atgatatttt aattatgggt gctcaattaa
    18781 atccattacc tttaaatgag catgatgaag taaatacaac aacaataata ggaaaaaaag
    18841 caaagaaacc tatgattata gaaaatccag tttatatttc ccacatgtca tttggagctt
    18901 tgtctaaaga actaaaaata gctttagcaa agggtgcagc ccaaaataag acagctatgt
    18961 gtagtggtga aggaggtata ttaccagaag agaaggaagc ttcatacaag tatatttttg
    19021 agtatgtacc aaataaatat agtgtaactg aagaaaatct taaaaattct gatgctatag
    19081 agataaaaat tggtcaagga acaaaacctg gtatgggagg tcatcttcct ggcgaaaaag
    19141 taactgaaga aatagcaaaa gtaagaaata agcctgtagg tcaagatgta ataagtcctt
    19201 cttgttttga ggaaatacaa tccaaagagg atttaaagaa acttgtagat gaacttagag
    19261 aagtttcaga agggcgtcca ataggtgtta aaatttcagc aggtcatatt gaaaaagata
    19321 tggaatttat agcttatgct aaacctgatt ttgtaactat tgatggtcgt ggtggagcta
    19381 ctggagcaag tccaaagttg cttaaagatg caacttcaat tccaacaata tttgctcttt
    19441 atcgtgctag aaaatacata gatacacatg gattagatat agatttggtt ataacaggtg
    19501 ggcttcgtat atctactgat tttgctaagg caattgctat gggggctgat gcagttgcta
    19561 ttgcaagttc tgctcttatg gcagcggcgt gtcaacaata tcgtatatgt ggttctggta
    19621 agtgtcctgt aggagtagct actcaggatg aggaattgag aaaaagatta catatagaaa
    19681 acagtgctaa tagagtggca aactttttaa atgtaagttt agaagaactt aagacttttg
    19741 ctagaatttc tggtcataaa gatatacatg atttaagtgt tgatgattta tatactgtta
    19801 actctgaaat atcaaattat acaaacatac agcatgtata gattgaagtt tgaaactatt
    19861 agattttaga taactataaa agtaaatgat aaatatattt tataaaagag agtgtcttaa
    19921 aatgaacttt ttagttcata agtcactctt ttttgtatga agccaaatat atttatcatt
    19981 ttagcaatga aaaagaatct tatctcttta ttttgagata gactccttct atttatgaaa
    20041 attaaagata ttaaattgta aaatattctg tgatgtcgta ctattaaact aaagattctg
    20101 tactttaaag taaataaaaa tttaaggagt gattattatg gaaagattga agatacaagt
    20161 tttagtcgac aataatactt atattgatag atactttgtt ggagaaccag cagtaagcta
    20221 ttatattgag attgatggaa ataggatttt gtttgactca gggtattcag atgtattcat
    20281 atcaaacgct gaaaaattaa atatagattt aggaaattta actcatgttg ttttttctca
    20341 tgggcataat gaccatacaa gaggtatcca gtttctagaa aataggtatg atttatctac
    20401 agttgaactt atttcacatc ctaactgttt tatacctaga aaacatggag agaaaagcat
    20461 aggagctcca ttttctgctg aggaaattaa aaatattttt atgtataatc ctaaggataa
    20521 gccatttaat ctaagcaaga attgtgtgtt tttaggtgaa atacattcta taaatgattt
    20581 tgaaaaaaga gctaaaattg gtaaatgtaa aataggagat ttgtgggaag acgattatgt
    20641 tttagatgat tctgctatag tttgtaaaac tgataaaggt atttttatta ttacaggatg
    20701 ttctcatagt ggaatttgta atatagtaga gtatgctaaa aaggtatgtg gagatgatag
    20761 agttattggt atactaggtg gttttcatct ttttgaattg aataataggt tagatagtac
    20821 aatacaatat ttggataaag aagaaataag aatgttgtac ccttgtcact gtgtttcttt
    20881 taaggtaaaa gcgaagatga atgaaatatt gaatataaat gaagttggag tagggctgac
    20941 cataagtata tagtatttga aagtatataa gatttaagtt gaaggttaaa aagatatttt
    21001 ctatagaaaa tatctttttt gttacgttta ttttaagaga aatcaatata cttttatatg
    21061 aaatattgta aaaaaattta tttttaatga gaatatattt tcattaataa acaattttat
    21121 tatgtattaa taatgttaga ataaatataa ataaatcaaa gttacttaca ttacagtata
    21181 ttttttcaca aaatatattg atttattctt ttaaagtttg ataattccaa tgaatgtaat
    21241 aaaagaagga attttatact atacgattat tattacaaaa taataaagaa aaaattatat
    21301 aataataaag aaaaaatgta tagtatttaa cattaaaaaa gatatttcat aaaattaaaa
    21361 aacaaagtga atttgaatac tatttaaaaa ataaaataaa tatataaaaa atatttaata
    21421 taaaggtaga aataagttga aaatgtaaag tcattggaaa aatatagttg aatttaatga
    21481 gcaaaaatgt tattgtttta tcaataaagt gttaaaataa aaactttcaa tatcatttta
    21541 gggaggatga tttcatgcaa aaaagtgtaa agaaagcaaa agtaacgggc tctatgttag
    21601 ccatttttgg tgttgcaagc gttcttttta gttcacatgc aggaggagga tttgctacag
    21661 ggaatcaaga aactcagtac tatgtgcagt atggatggac agcaccgctt atggcaattt
    21721 tagcaatgat tatacttact gctaccatga gagaagttat cataatgtat aataataaca
    21781 attgtaggaa ttataaggat ttattttgtg agctttggag accatatcca aaacttgaaa
    21841 taatatggga gatatattac tatctaatgg ttttaatagc tgtaagtgct gttatagcag
    21901 gtgcagcagc agtatttcaa agtataggag taaattattt tgtagctgta tttattattg
    21961 gagttgtact tcttgtcttt actatatttg gtgctatgct tgtatctaaa gcagctacag
    22021 caatgacaat agctattcta gtatgtactc ttactatttt tattgttggt ataaaagcta
    22081 aagtaccaga aataactgaa atattatcaa atagaacgag tttcacacct ggctatatca
    22141 agccaatact taatactttt atttatgcag gttttcaatc ggttgttata cctacacttg
    22201 caggatgttc aagaccactt cttaagaact caaaagaagc tactaaggca atgataattt
    22261 catttgttat gaatgcaatt gcattaggac ttgctgtaac tatgctaatg ggatggtata
    22321 gagaaattat tgctgctggg caaacaacac tccctactct ttatgttgca ggtcaatctg
    22381 gaaatcatac tatatacata atttataatg ttgcattatt cctttgttta atgtctacag
    22441 gagtaactac tatatttggt cttgtaaata gatttgaaga ccataaagca ctttctttcc
    22501 tttcttcaag aatgaaaaga agggtattta cagcttgtgc aataatggta gtgtcaatgc
    22561 ttatatcact tacaggatta agtaatattg ttaaatatgg atatggatat tgtggttatc
    22621 ttggtctatt cactatagta attccattcc ttacactagg acattataag aataagaagt
    22681 ttgcaaagga aaatccagaa gctaaatggc cagcggaatt aaatgaaaat gtaaattaga
    22741 tgtagtagca aaaaatattg ctaaagagaa tactataaat aagaatacta tagataatat
    22801 atctgcatgt ttattaagat tatttgaaaa atctaatatt aaacaactat aaacaagcaa
    22861 tataaaacta agaataaaag ataaaaagag ggtgtctcaa aatggacttt tagtttataa
    22921 ggtactcttt tttatatgaa gtcaaatata ttttcattat tttgacaata aaagagacta
    22981 atcctacttt ggatagcctc ttttttcttt aatataaaat agtataacag caaagtatag
    23041 attgatacat gttttctgta gctttctata tattacagtg tttgtgtggc attggtattt
    23101 taagcaatct ataaattata attctataat tcttctacta attttagatt ctatagccgt
    23161 ctctatttca cttaacattc gttcattttt agggtcaata aaagcacatg tatagccttc
    23221 ttcaccagct ctaccagttc taccaatacg gtggatgtag tcttctggtg tttctggtaa
    23281 atcgtaatta tatatatgac ttactccaga tatatcaagc ccacgagaag ctacatctgt
    23341 agctattaag tattgtatat ctaaatttct aaatgatttc attattcttt ctcgttttga
    23401 ttgagcaata tcactatgaa gtttttgaca attatatcct cttatagcaa gagcttcttc
    23461 aagattgtca acccttctct tagttctaca aaatattatt gccataaaag gattatcttc
    23521 atctagtacc ttacataagg catctaattt ttttctatct gtagtttcta ctacttcttg
    23581 ttttatatta ttaagagtga tttcttcttt ttgaatagaa actacaacag gattttttgt
    23641 gtatctataa gctaattttt ttacttgaga atttatagtt gcagaaaagc atagtgtttg
    23701 ctttttgttt gaagtctctt ttaaaatagc ttccacttca tttttaaatc ccataagcaa
    23761 catttggtct gcttcatcta aaacaaaagt cttcaactta ctaaggtcta ttgtttttct
    23821 atttagatgg tctaaaagcc tacctggtgt agctataatc atatgaatat ttccttttag
    23881 tttctttatt tgagaaccaa tatcttttcc tccatatgct gcaagaatac ttacatcttt
    23941 agattcttta agtttcatag cttcttctgt tatttgaata gctagttctc ttgtaggaga
    24001 aagtataagt acttgtatat catttatatc taatgatata ttttcaaata tcggaagtaa
    24061 aaaagctagt gtttttcctg ttccagtttg agcttctgct attacatctt ttccttcttt
    24121 tattagttta atactttctt tttgtatagg tgtaggtgtt gttattcctg atttttttag
    24181 aatatttatt gtattttcac taatccctaa gcttttgaaa tccattgtta tcttaccttt
    24241 catgaaaaat tttctttttt attaaccaat taataatatc atatttgtaa gtatttttag
    24301 taaatttttt aattattatt tatatagctt aaaaatatat taagatactt taaaatggaa
    24361 tgtaacttaa attaaatttt agtaggaatt ttataggttt atttttaaag gttttttgaa
    24421 aataaatcta tttaaaaatt aaattaaaat gcaagaaaaa cataataact ttcattttta
    24481 agaagttttc acaaaaaaga aaaaatatag tgcaataaat gatgcaaagt aatataatag
    24541 attacaagat atgaaaagct aaaaaaataa attgcaaatg aaattaaaat gaataaaaaa
    24601 taaaaaaata atcataagtt agaggtatca atgaaaaata agacttaatg tttttaaaat
    24661 taaaagtata ctaagggggg cattaatatg tgcgttatat caaaagatag agcaaaggaa
    24721 ataaaaatag tagatactac tttaagagat ggagagcaaa cagctggggt tgtatttgca
    24781 aatagagaaa aaataatgat tgctgaaatg ttaagtgacc taggtgttga ccaaattgaa
    24841 gttggtatac ctactatggg tggagatgaa aaaaatgtaa taaagcacat atgtagtaga
    24901 aatttaaaat cagatataat ggcttggaat agagcagtaa taaaagatgt tgaagaatct
    24961 atttcttgtg gtgttgatgc agtagctata tcaatatcag tatctgacat tcatatagaa
    25021 aataaattga gaacttctag aggctgggtt ttagaaaata tggctaaaac agttgaattt
    25081 gcaaagaaaa atggattata tgtttcagta aatggagaag atgcttcaag agcagatata
    25141 gacttcttaa ctgaatttat aaatgttgga aaacaagcag gggcagatag atttagatac
    25201 tgtgatacag taggagttat gaatcctttt tctataaaga atgcaataga aactctttat
    25261 gaaagaacta attttgatat agaaatgcat acacataatg actttgggat ggcaactgca
    25321 aatgcacttg caggaatagc agcaggagca aattatgttg gtgttacagt aaatggatta
    25381 ggtgaaagag caggaaatgc agcattagaa gaagttttaa tggcacttaa gtgtgtttat
    25441 aaatgtgatt taaataatat agatactaga aaatttagag gaatatgtga gtatgtagca
    25501 caggcatcag gaagaatact tcctacatgg aaaccagtag ttggggataa tatgtttata
    25561 catgaatctg gtatacatgc agatggagct ttaaaagacc cacataacta tgaaccattt
    25621 gacccaagtg aagtaaatct tgaaagaaaa atagtaatag gtaaacattc aggaagagct
    25681 gcagttgtaa ataagttgag tgaatatgaa atgtatattt ctccagaaaa cgctaccaag
    25741 ctattaaatg ctataagagc tacatctata agattaaaaa gaagtttaat ggataaagaa
    25801 attcttcaat tatattgtga tatactagca catgagaaag gcacaacaga agaagaagca
    25861 gttagaggtt catatattta agatataatt gtttaggagg aaaatatggg agataatata
    25921 gtttataaaa taattaaaaa acatatagta gatggagaag ctgttgctgg aagttcaatt
    25981 ggtataaaaa tagaccaaac tttaacacaa gattctactg gaacaatgac atatcttcaa
    26041 ctagaagcta tgggaataga taaagtaaag actaaaagaa gtgtggcatt tgtagaccat
    26101 aatatgttac aacaaggatt tgaaaatgca gacgaccata agtatataca aacagtggca
    26161 gataaatatg gagtttattt ctctaagcca ggaaatggta tttgtcatca agtattttta
    26221 gaaagatttt caacaccagg agatacactt ttaggttctg atagccacac acctactgct
    26281 ggaggtgtgg gcatgatggc tattggagct ggtgggttag atgttgcttt agccatggca
    26341 ggaggagcat actatataaa agcaccaaaa gtctgtaagg tgaatctagt tggaaaatta
    26401 aataatatgg tatcatcaaa agatataata ttagaagtgt taagaaaaca aactgtaaaa
    26461 ggtggagtag gtaaagttta tgaatatggt ggagaaggtg tcaaaagcct atctgttcca
    26521 caaagagcta caataacaaa tatgggggct gaacttggag caactacttc aatcttccca
    26581 agtgatgaaa aaacactaga attttttaag agtcaaggaa gagaagacgc atgggtagag
    26641 ctaaaaccag atgcagatgc agtttatgat gaagagatta ctataaactt ggatgaatta
    26701 aaaccattag ctgcaaaacc tcatagccca gacaatgtag atgaagttga aaatataggc
    26761 aagattaaaa tagaccaagt ggcaatagga agttgtacaa attcttctta tgaagactta
    26821 atgaaagtag cacaaatcct gaaaggaaat aaagtacata aggatgtgag tttggtaata
    26881 gcaccaggtt caagacaagt tatggagatg attgctagaa atggggcttt ggcagatata
    26941 ataagtgctg gagctagaat actagaaaat tcttgtggtc catgtattgg tatgggtcaa
    27001 tctccaggta ctgactctgt gtctttaaga actttcaata gaaatttcta tggtagaagt
    27061 gggactttgt cagcacaagt atatttagta agtccagaag tggcagcagt aagtgcaatc
    27121 aagggagtgc ttacagaccc aagagaattt gatattaaat ttacaaattt agatgtaaat
    27181 gagtttttga tagatgatag catgataatt aaacctgctg atgtaggaag tgatgtagaa
    27241 gtagttagag gacctaatat aaaacctttc cctcttaata cagagctaag tcagtctata
    27301 ggtggaaaag ttatattaaa gacagaagat aatataacta ctgaccatat aatgccatca
    27361 aatgctaagt tacttccttt tagaagtaat ataccgtatc ttgcaaacta ttgtttcaat
    27421 actgtagata cagaatttcc tcagagagca aaagacaata atggtggatt tatagttggt
    27481 ggagataatt atggtcaagg ttcaagtaga gaacatgcag ctttagcacc attatatcta
    27541 ggtgttaaag gggtaatagt taagagcttt gcaagaatcc acaaggctaa cttgataaat
    27601 agtggtataa ttccaatgga gttttgtgat gagaaagatt atgaaaatat atctctttta
    27661 gataatctgg agataccaaa tattttagac aatctaggta gtggaatttt agaagttaag
    27721 aatacaacta aaggaacttc ttttaaggtc aaagtagaat tgtcagcaaa agaagttgat
    27781 gtattaaaag ctggtggaaa attaaactac actaaaaatc aagctaatta atatgaaaag
    27841 agggaatatt gaatgtataa agtgacactt ataccaggag atggaatagg accagaagtt
    27901 gcgaaagcta tgaaaaaggt agtagaagct actggagtag aaatagaatg ggaagaagtt
    27961 aatgcaggag aggcagttat tgaagagtat ggtactccac ttcctgaata tattatagat
    28021 agtataaaaa agaacaaaat agctataaaa ggcccaatta ctactcctgt aggtaaagga
    28081 tttaggagtg taaatgtaac tttaagacag gcattagatt tgtatgtcaa tttaagacct
    28141 attaaaagtt ttaaggggat aaaatcaaga tatgaagatg ttgatttagt ggttgtaaga
    28201 gaaaatacag aagatttata tgctggaata gaacataaaa taggtgatta tgcagctgaa
    28261 agtataaaga taataacaag aagtgcaagt gaaagaatcg ttgattttgc atgtaactat
    28321 gttaaagata ataagagaaa aaaagttaca gctatacata aggcaaatat aatgaaaatg
    28381 tctgatggat tgtttttaga tgtgtttaga gaagttgcat caaaacatgg tgtagaatat
    28441 gatgatttga ttgtagatgc agcagctatg aacttagttt taaatccaga aaattatgat
    28501 gtaatggtaa tgccaaatct ttatggagat atactatcag atttaggtgc tgggcttgtt
    28561 ggaggtttag gtataattcc aagtgcaaat attggtaaag attgtgcgat atttgaagct
    28621 gttcatggaa gtgctccaca aatagcaggt caaaataagg ctaatccaac agcacttatt
    28681 caatcatcag taatgatgct tagatactta ggtgaatatg aaaatgcaca aaaaatagaa
    28741 acagctttag aaaaagtatt tcttgaagga agtaaattga ctgtagattt gggtggctca
    28801 gcttctacta cagagtttgc ggatgaggtg tgtaagtata ttgtatagat tttatttagg
    28861 gaaaaagtaa aatatagatt gaaggaaaga ggctttctca aaatagagat aagccttttt
    28921 tattggaatg ataaaatata tttgatttta tacaaaagag agttcctcat aaacgaaaaa
    28981 gttaattttg ggatatccat tttaatttta aaattaattg attaatatgc aaagaaagat
    29041 taaataaatt tattattttt aattgaactt tcaaccaaaa tatggtaaaa tttaaaaaag
    29101 ttgaactttc aactaaaaat gaatatattg attatgtgaa attctataaa acagaaacta
    29161 tttgacttta gccatagtag tttcagtgga ggtgtgtttg attaagactt tagacagtaa
    29221 tatattaaga gaagtaggta ctttatctag agcagtaaat tcaataaatg atattaagta
    29281 caaggaatta aagttacaaa aaggacaatt tacatttttg acaagaatct gtgaaaatcc
    29341 tggtataaat cttgtggaat tatcaaatat gttaaaggta gacaaggcta caacaaccaa
    29401 ggctatacaa aaattaataa aggcaggata tgtagataaa aagcaggatg agtttgataa
    29461 aagaggatat aatttaactc caactgataa atctttggaa gtttatgagt tgataattga
    29521 agaagagaat agaagtatag agatatgttt tgataatttt acagatgaag aaaaacaagt
    29581 ggtaactaag ttattagaaa aaatgagcaa aaacgtagaa aatgaatggt ttaaagtaaa
    29641 gagatagtaa ataaatacat taacttaagg agatattttt atgattagaa aagcgaatat
    29701 gaatgattta gaaagcatta tgaaaattat aaaatctaca gtggaagaaa tgaagactta
    29761 taataataca caatgggatg aaaactatcc attagaaaaa gactttgttt cagatattaa
    29821 gaaacaagat ttatatattt atgaagtgga tggagaagta gcagggttta tatgtcttaa
    29881 ttatgaagag ccagaagaat atttaggctt aaattggtca tctaataaaa aagcaatggt
    29941 aatacataga atggctgtaa atcaaaactt tagaaagaca ggaatagcat ctaagttagt
    30001 tgattttgct gaaaaattag ctgtagaaaa caatgtatca tacttaaaga gcgatacata
    30061 ttcaataaac tcaaaaatga atttattact tacaaagtgt ggttttataa aaatcggaga
    30121 aatgagtttt ttaggaaagg agaaatcttt ctactgttat gataaaatat tataaagagc
    30181 atttacaata caaggggtaa ataatagatt ttcagcatta gtaagggcat aatataacct
    30241 agtatatgct attatgaaag ggatgattta tatgattgga tttctaaaga aaagaagaag
    30301 tattagaaaa tacaaggatg ttgaagtaga aaaagaaaaa ttagataaaa tattaaaagc
    30361 tgctctttta gctccatcat caaaggggct tagaacatgg gaatttatag tggtagatga
    30421 taaagaaaag ctaattaatt tatctcaatg tagaactaaa ggtggtggat ttttcttaaa
    30481 aaatgcacct ttagctattg taattattgc agataaagaa aaaaatgatg tttggataga
    30541 ggatgcaagt atagctgctt catacataca acttcaagct catgaactag gtttaggttc
    30601 ttgttggata caagtaagaa atagaatgca tgatgataat atagaagctg ataagtacat
    30661 tagagaagaa ttaaaagtac ctagcaagta cagtgtagaa tgtataattt ctataggata
    30721 tcctgatgaa gaaaagaaag catacggtga ctctgattta gactataaaa aggttcattt
    30781 taacaacttt tagtatataa tatgttgtat taagacatta tatttttttc tattgttaat
    30841 atatacttaa aattgtaaaa atatatctga attttactaa cttatctagg ggttatgtac
    30901 aaggatttta aagataaata tagaaattaa tgtttataat cgatacataa cttgagtaac
    30961 aaaatttaag gaggttaaag tatgggtata tttaaaacaa aaatggatga ggactggaaa
    31021 gtaaactaca ttaaagagtt taatgaaatg agagattctt atgagagtaa acttcaaaaa
    31081 aaacagtttg aggtagatag tttgaaatca gaattggata gactaagaag ctataaaaat
    31141 agccttaagc caaaagaaaa acaaattact gatgatgata taaataatat aaagagttta
    31201 agaagagatg gcttaagcta taaagaaatt tcaaatcaaa caagttggag caaagcaact
    31261 gtaagtaggg tattaaatgg actttacgat tagcatatac atggggtgat ttaaattggg
    31321 atgaagtata gttacaaaaa atgcataata gattctattt tattgatgtt tatagttcaa
    31381 atattaagga tgattttgaa ttatgtactt ctaagtcaat ttgaatttac tcttgaaaat
    31441 tttaatatta taaacttaat ttcttttaca ttagttggtt tatcgttaat tttattttta
    31501 aaagataatt ctttgtataa caaggttaga aatagaaaaa tcacagaagc ttttgaagaa
    31561 aataaaaaca atatacttat tgaaaagtgt aagctgatac tttttgttgt ggttttatct
    31621 ttagcaataa ttgtaactta ttgtactaag ggatatgtac tttttaatgt aactatgatg
    31681 actttgtctg tattgatagt tccaatattt gaagagttat tttttagaga atatatatgg
    31741 aactatctaa gtaattttat aaagtcaaag ggtaaaatta tctgtataac atctatacta
    31801 tctggaattt ataatatagg atacatagat gttataagaa attatgtaat attgtataac
    31861 aattcaagct acacctttga agttattatt tcaaaaatta tgataggtac tgtttttggt
    31921 attgtacttg gattagttaa gtataggttt agagatgtag ggttttgtat attattgagg
    31981 agcttatttg ctatatttat taggtagata gtataatcgg tttataaggt tgttttaaat
    32041 ttaattttaa atcaacctta ttttatttgt g
//
LOCUS       DAFKMX010000020        31789 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFKMX010000020 DAFKMX010000000
VERSION     DAFKMX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.020"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            218..535
                     /gene="flgB"
                     /locus_tag="LZB08_000816"
     CDS             218..535
                     /gene="flgB"
                     /locus_tag="LZB08_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBM7314331.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            543..947
                     /gene="flgC"
                     /locus_tag="LZB08_000817"
     CDS             543..947
                     /gene="flgC"
                     /locus_tag="LZB08_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBM7314332.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            957..1274
                     /gene="fliE"
                     /locus_tag="LZB08_000818"
     CDS             957..1274
                     /gene="fliE"
                     /locus_tag="LZB08_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBM7314333.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1299..2849
                     /gene="fliF"
                     /locus_tag="LZB08_000819"
     CDS             1299..2849
                     /gene="fliF"
                     /locus_tag="LZB08_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBM7314334.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISIDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEQEDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2862..3932
                     /gene="fliG"
                     /locus_tag="LZB08_000820"
     CDS             2862..3932
                     /gene="fliG"
                     /locus_tag="LZB08_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBM7314335.1"
                     /translation="MSTEITNVNAFDLSDISESDKNEKIEGEGLKKAAVLLMTLGPDV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAIRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDAISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIVNS
                     VRKLEREGAITIVRNFGDDFVE"
     gene            3916..4656
                     /locus_tag="LZB08_000821"
     CDS             3916..4656
                     /locus_tag="LZB08_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBM7314336.1"
                     /translation="MTLLNNKIIKSKKASYKGVLDLSIIDKVRVGESNLNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFKLFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            4660..5979
                     /gene="fliI"
                     /locus_tag="LZB08_000822"
     CDS             4660..5979
                     /gene="fliI"
                     /locus_tag="LZB08_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBM7314337.1"
                     /translation="MYKTDIYIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6010..6456
                     /gene="fliJ"
                     /locus_tag="LZB08_000823"
     CDS             6010..6456
                     /gene="fliJ"
                     /locus_tag="LZB08_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBM7314338.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6485..7690
                     /locus_tag="LZB08_000824"
     CDS             6485..7690
                     /locus_tag="LZB08_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBM7314339.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENII
                     DDDRVDLLA"
     gene            7708..8316
                     /locus_tag="LZB08_000825"
     CDS             7708..8316
                     /locus_tag="LZB08_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBM7314340.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8343..9326
                     /locus_tag="LZB08_000826"
     CDS             8343..9326
                     /locus_tag="LZB08_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBM7314341.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQIYAFQNPAGLDAAGGNLFKPSANSGNPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9339..9542
                     /locus_tag="LZB08_000827"
     CDS             9339..9542
                     /locus_tag="LZB08_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBM7314342.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9542..10360
                     /locus_tag="LZB08_000828"
     CDS             9542..10360
                     /locus_tag="LZB08_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBM7314343.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10350..11045
                     /locus_tag="LZB08_000829"
     CDS             10350..11045
                     /locus_tag="LZB08_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBM7314344.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11057..11500
                     /locus_tag="LZB08_000830"
     CDS             11057..11500
                     /locus_tag="LZB08_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBM7314345.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11515..11889
                     /locus_tag="LZB08_000831"
     CDS             11515..11889
                     /locus_tag="LZB08_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBM7314346.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11890..12555
                     /gene="fliP"
                     /locus_tag="LZB08_000832"
     CDS             11890..12555
                     /gene="fliP"
                     /locus_tag="LZB08_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBM7314347.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12568..12837
                     /gene="fliQ"
                     /locus_tag="LZB08_000833"
     CDS             12568..12837
                     /gene="fliQ"
                     /locus_tag="LZB08_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBM7314348.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            12849..14663
                     /locus_tag="LZB08_000834"
     CDS             12849..14663
                     /locus_tag="LZB08_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBM7314349.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14672..16747
                     /gene="flhA"
                     /locus_tag="LZB08_000835"
     CDS             14672..16747
                     /gene="flhA"
                     /locus_tag="LZB08_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBM7314350.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16722..17738
                     /gene="flhF"
                     /locus_tag="LZB08_000836"
     CDS             16722..17738
                     /gene="flhF"
                     /locus_tag="LZB08_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBM7314351.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17735..18613
                     /locus_tag="LZB08_000837"
     CDS             17735..18613
                     /locus_tag="LZB08_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBM7314352.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKFTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18627..19328
                     /locus_tag="LZB08_000838"
     CDS             18627..19328
                     /locus_tag="LZB08_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBM7314353.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19344..19766
                     /locus_tag="LZB08_000839"
     CDS             19344..19766
                     /locus_tag="LZB08_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBM7314354.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19751..19936
                     /locus_tag="LZB08_000840"
     CDS             19751..19936
                     /locus_tag="LZB08_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBM7314355.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19977..20750
                     /gene="flgG"
                     /locus_tag="LZB08_000841"
     CDS             19977..20750
                     /gene="flgG"
                     /locus_tag="LZB08_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBM7314356.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20771..21532
                     /gene="flgG"
                     /locus_tag="LZB08_000842"
     CDS             20771..21532
                     /gene="flgG"
                     /locus_tag="LZB08_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBM7314357.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21555..22436
                     /locus_tag="LZB08_000843"
     CDS             21555..22436
                     /locus_tag="LZB08_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBM7314358.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVRDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22439..22813
                     /locus_tag="LZB08_000844"
     CDS             22439..22813
                     /locus_tag="LZB08_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBM7314359.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22845..24248
                     /locus_tag="LZB08_000845"
     CDS             22845..24248
                     /locus_tag="LZB08_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBM7314360.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24364..26301
                     /gene="htpG"
                     /locus_tag="LZB08_000846"
     CDS             24364..26301
                     /gene="htpG"
                     /locus_tag="LZB08_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBM7314361.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26688..27818
                     /locus_tag="LZB08_000847"
     CDS             26688..27818
                     /locus_tag="LZB08_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBM7314362.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28045..29067
                     /gene="splB"
                     /locus_tag="LZB08_000848"
     CDS             28045..29067
                     /gene="splB"
                     /locus_tag="LZB08_000848"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBM7314363.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29249..30160
                     /locus_tag="LZB08_000849"
     CDS             29249..30160
                     /locus_tag="LZB08_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7314364.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPVKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30175..30591
                     /locus_tag="LZB08_000850"
     CDS             30175..30591
                     /locus_tag="LZB08_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBM7314365.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30691..31083)
                     /locus_tag="LZB08_000851"
     CDS             complement(30691..31083)
                     /locus_tag="LZB08_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314366.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31224..31619
                     /locus_tag="LZB08_000852"
     CDS             31224..31619
                     /locus_tag="LZB08_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBM7314367.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13021 a         3359 c         5873 g         9536 t
ORIGIN      
        1 cgaagaaata tttaaataca tattaaaatt tttttatatt ttcatgggac ttcattaaaa
       61 aaatattagt tttcttacta aattgataca ttattgtatt aatgtaatgg aaatgcgatt
      121 aatttaaata aataaagaag tcattttttg taatatagca acttcatttt ttattaataa
      181 acaaaataga taaaatatat tttgaggagg ggtgagtgtg aatacatatg acattataaa
      241 aatgggatta gatgcaacta atctaagaag tcagacaata gcaaataata tagcaaatat
      301 aaatactgaa ggatataaaa gacattatat aacttttgaa gaaacactaa atgatgctat
      361 gcctaaaata gaagaaaaaa cagacaattc taaaggttta agagtagatg gtaataatgt
      421 agacatagaa gaagaaaaag taaatcaagc aatggcaaca ttacaatata atgctttgat
      481 aagttttgca aatggaaaaa tagcaatgac caaatcaata ataagtggga ggtaattaga
      541 agatgagtgt gttcagtgga atgagaatat ctgctagtgg attaacagca gaaagtttga
      601 aaatggatat catatcatcc aatgtagcta atataaatgc tacaagaggt ccaaatggag
      661 aagcatatag gagaaaagtt gtttcatttg aagaaaacta tgataataaa ttaggtatgc
      721 taggagtaaa agttacatct gttgaagatg ataaatcacc tcttaaagaa gtatatgacc
      781 catctcatcc agatgcaaag ccaaatggat atgtagaata tccaaatgta aatatattgg
      841 tagaaatgac agatttaata acagcttcac gagcatatga ttctaacgta gatacattga
      901 atgctcagaa gtctatgata tcaaaagctt tggaaatagg aaagtaggga taaattatgc
      961 caattacaca aattaacaat ttaatgcaag gtgcaaagtt agataatttg attggagaag
     1021 caaagccatt aactcttaat aataaagaag aaaaaaagaa ttttgaagat gtaataatag
     1081 atactataga taaagttaat tttcaacaag taacagcaga tgatatgaaa gaaaaattta
     1141 taaaaggcga agatgtttct atgcatgaag ttatgttaat aggtcaagaa gcacaactat
     1201 ctatgcaata tttaatagaa gttagaaata agattcatga tgcatatcaa gaaatgaata
     1261 aggtgcaaat ttagcctttt gaacaggagt aataatcaat gataaccagt tttaaaaata
     1321 agattaagga gcaagttgct aaatctagga caaagataag ttctttaaaa aagggaacta
     1381 aaatagcatt aggtataggg gttattgcta tatgtttagc gctagtattt acagcaatat
     1441 ctgcgagcaa aaataagtat gcagtcttat tttcagaact agatgctcaa gatgctgcag
     1501 atatatcagc agagctagag tctaaaaaga tagatactaa ggtaaaagga agtacgattt
     1561 atgtacctag aacagaagtt gataagttaa ggctggagtt agcgccaagt cttacaggtg
     1621 gttctaaagg atttgcacta atggatgata gttcatcaat agggcttact gatgaagaat
     1681 ttcagattaa gaaacaaaga atgatacaag gtgaaataga aaaaacaata aagagttttc
     1741 cacaagtgga agacactaga gtacatataa catttggaga gtcatcagta tttgagaagg
     1801 aggctgttcc agggagtgca gcagtttatt tgatgctaaa accagggacg actttagaaa
     1861 aaaaccaaat aaagtctgtt atggcactta tatcaggaag cgttacaaat ataccagaga
     1921 aaaatgttca ggttattgat aacaaaatga atttattatc tgaaggtatt ttggaggatg
     1981 aaaatgttga tgaaaataat caaccaataa atagtggcat tagtattgat aaaaataagg
     2041 catctgaaca agcattgaat aaacaactag aaaagtctat tttagaaatg ctagaaccga
     2101 tatttggtaa gggaaaggtg aaagctacag taaatgctga tttaagtttt gatacagctg
     2161 aaaaaactga gataaaaatt gaccctgata aagttgcaat aaaggagaca aggtcgaaaa
     2221 attcatctaa atctgctgca aataatgttc aaggtgtaga tgctaacatg aataaccaag
     2281 gaaataacaa tggtactaat acagaagata gtttagatga gagttttgag tatgaaacag
     2341 gtaagattga aactcacact atagttgcgc ctggagaact taatagattg acagcttctg
     2401 ttgcaataga tggaaaaata gctaaaggtg ttcaagctga tgtagaggat attgttaata
     2461 atgctatagg tatggatgga gcgaggggag atactcttag cgttgtttca atggtatttg
     2521 acccagaagg taagaaagag gctaaacaag agctggaaga gattaaaaaa gaaggattta
     2581 aaaagaacat aataaaagca gttattggag tagtagcagt aatagcagtt ggagctattg
     2641 gatacttaat atataagaaa agaagtgaac aagaagatat ggattatgaa gatgaagatt
     2701 atatttctaa atctgattct gttgatagga aagataatcc tctattagga gatgacccag
     2761 aactgacatt agaggaagct gttaaaatgt atgcagaaga aaagccagac caagtaacag
     2821 agataataaa aacttggtta aatgagtaag ggggatagaa agtgtccaca gaaattacaa
     2881 atgttaatgc gtttgactta agtgatataa gtgaaagtga taagaacgag aaaatagaag
     2941 gtgaaggttt aaaaaaagct gctgtattat taatgacatt agggccagat gtctctagcg
     3001 atataataaa aagtttgcct gataaacaag ttcaaaaaat aggtttggaa attgcaaata
     3061 tttcttcaat agcttctaaa gaaagaagag aaatcttaaa agagtttgta gaaataaata
     3121 gagcaaaaga ttatgttata gaaggtgggc ttgaatatgc aagagagtta ttaagtaatg
     3181 ctttgggaaa tgctaaagca aacaaattac ttgaaggtat atcaataaat acaagtacaa
     3241 agccttttaa acatattatc aaactagatg taaagaagat attagaaata cttaggaatg
     3301 aaagtgcaca aactatagcc gttttacttg cacatatgga accagaaaat ggggcagaag
     3361 ttttatcata tttagacttt gatttacaaa atgaagtagc tattagaata agttcaatgg
     3421 gttcaatatc tccagaagta ataaaggtaa ttgaaagttc attagaagat aaattacatt
     3481 caataagtgg tgatgatttt aaggattatg gaggagtgtc tacattagtt caaatattga
     3541 ttaattctga tagaaaaaca gaaaagaata tcttgggaac tattgaagaa caaaacccgg
     3601 atttagcagc tcaaattaaa tctcatatat ttgtctttga agatattgtc aagttagatg
     3661 atataagcat acaaaaagtg ttaaaagaag taactatgaa agatttagta tatgccttaa
     3721 aaggtgtgga tgatgcgatt tcagatgcta tatatagaaa tcaatcttct agggcagcag
     3781 aggcacttag agaagaaatg gaaatggttg gtgttataaa ggttacacag gttgaggctg
     3841 ctcaacaaaa aatagtaaat agtgtaagaa aacttgaaag agaaggtgcc ataactattg
     3901 taaggaattt tggtgatgac tttgttgaat aataagatta ttaaaagtaa aaaagcatca
     3961 tataaaggag ttttagattt atccataata gataaagtta gagtgggaga atcaaatcta
     4021 aataaagaaa atgattgtag atttagggat gaggtaataa aggaagctat agaatctgca
     4081 gagataaaaa agaatgagat attagaagaa gcaaaaatga acgcacaaaa tatagaaaag
     4141 caagcatatg aaaaagggta tagtcaaggt caaaaaaatg gatatgagga tggatataaa
     4201 gagtcatatg atgaatatac ccaaaaagct aaagatgaag cagattttat aaaaaatcag
     4261 gcaaatttaa tcttattaca agctaatgag aagatgactg agtacataaa agaaaaaaat
     4321 aaagagattg taagacttgc tattaatatg gccagttctg ttttaaaaaa gcatttcgaa
     4381 gatgaagaat ctatgagtga tttgttaaag caaattattc aagattatga aggaagtagt
     4441 tcatttatag taagatgtaa ttctttctat aaagataata tagagaaaga atttaaatta
     4501 tttaaagagg aaacatcact aaaatcaagt gtatttgtta ttgcagatga tagtatagat
     4561 aaaggaaatg ctattatcca aaaggaaaat ggaagaataa tagttggtat tgattgtgct
     4621 atggaaaaac taaaagaaga attgcttggt gaataaagta tgtataaaac tgatatatat
     4681 atagattttg acaaaataaa taagaaaata gaaaatttag aactcactat atcagaaggt
     4741 agagtaaaga agataatagg acttactgta gaagttgaag gaataaaagc ttttgttggg
     4801 gagttatgcg ttatatacaa ccaagttaat aaaccagtga attgtgaagt agtaggtttt
     4861 aaagataaag aagtcatatt aatggcgctg ggagagttaa cattgatagc tcctggttgt
     4921 aaagttattt ctaaggggat acctttaagt gttatgtgta gtgataattt actaggtaaa
     4981 gtacttgatg gtcttggaaa tcctatagac aactcagatg ctgttttagg agatagatac
     5041 aacctgaaca atgaacctcc tgacccaatg aagagaaaaa agatacgaaa tataatggaa
     5101 acaggtgtaa gagcaataga tgcatttact acttgtggtg aaggacagag aatcggcata
     5161 tttgcaggaa gtggagtagg taagagtacg actctaggta tgattgctag gaatgcaaaa
     5221 gcagatgtaa atgtaattgc tcttattgga gaaagaggaa gagaagtact agattttatt
     5281 gataaagatt taggtgaaga gggaatgaaa aaatctgttg ttgtgtgtgc aacatcagat
     5341 aaagcccctc tagttaggtt aaaaggtgct cttactgcta ctgcaatagc agaatatttt
     5401 agagaccaag gcaaaaaagt aatacttatg atggattcag taactagatt tgctatggca
     5461 caaagagaag ttggtgtagc aataggagag ccaccagctc aaaaaggata tacaccatct
     5521 gtatttgcaa tgttgccaaa acttatggag agaacaggga catcagataa aggttctata
     5581 actgcatttt atacagtatt agttgatgga gatgacttta atgagccaat agcagataca
     5641 acaagaggga tacttgatgg tcatatagtt ttatctagag acttggcaaa taagaatcac
     5701 tatccttcta tagatgtatt aaacagttta agtaggctta tgaatgaaat tgcttcaaaa
     5761 gaagacataa aaatagcgtc ttttgcaaga gacatgcttg ctgagtatag agaagcagaa
     5821 gatttgataa atataggtgc atatgcttca ggtacaaata aaaaaataga tgaagcaatc
     5881 tattatcatg agcatattat taatttttta aaacaaggga taaatgaaaa gagttctttt
     5941 aatgaaacta taagtagttt gagaagagtt tttgaataaa caattaaaaa aatatttgaa
     6001 taggttgtga tggaattgga taaaaacttt aagtttagat tacagaaagt tttagactta
     6061 aaagtgaaag atgaagaaga gataaaaatg gaatttgcta agattcaaca aaaaaaaata
     6121 gatatagaga ctaatttaga aaacttagaa agtaattata gcaaatattc tataagtaaa
     6181 aataacgata gtattcaaaa tcaaaaaata actattaact atttactagc tttaaacaat
     6241 tctataatgg atttatcaga agagttggat aagtcaacaa atgaacttga aaaagctaga
     6301 aagcagctta taagcaaaca gatagagaga aaatcattag aaaaattaaa agaaaaaaag
     6361 tatggacagt attataaaga agaaaattta aaagaacaaa acactaatga tgagtttgca
     6421 agtatgagtt atttaaggaa tagacaagtt ctataagaat agtaggaagt gaggatatta
     6481 ttgtatgaag atacaagcag attcttataa tattattaaa gatattgggg atagaaaaag
     6541 ttcatctaag aacaaaaata taaaaaataa taaatttgaa gataatctat ataaggcaac
     6601 agaccataaa aaggttaaag atagaaggga agtaagtaag caaaatatta gctcagataa
     6661 accggaaaat agtgataatg agtgtcattt agatgaagat aaaagtaatc acttagaaga
     6721 tgaaaataca tatgaaaaag attgtagtaa gaataatgaa gctttgcagc aattactgaa
     6781 tcttttaaag gataattata aaaaagataa agatataaac actatattga ataatataga
     6841 aaaactaagt atatcagatg aattaaaaca agaacttaag cttaataaaa atatattaaa
     6901 taagattgca aatcaaaatt cagagctaaa taaagctaat ataaataact ctaatgatat
     6961 agaaaattta gacatagtta aattaagaga acttttggaa gataatgcta ataaagatat
     7021 tgaagtaatt actaaaacta cagaaaatga taccaaaaat atagatttgt atagttttga
     7081 tagcaataga atggataatc ttaactcaaa taaacagaaa gatgatagct ctgatatttt
     7141 ggaaaaacta gcaggggtaa atggaaataa gagtgggaat tttggacaag taataaataa
     7201 aaatgttgat atgaataaaa ttaaaaatga taatgtcaat ttaaaacaga tagaatcaaa
     7261 tgtaatggat gactctataa aagctattaa gcatatgaaa acaaatgata tacaagaatt
     7321 gacaattaaa ttaagaccaa aagaattagg agatatgaat atacaattgt taaaagatgg
     7381 tgaaagtatg agagctgttg tgactgtttt caataaagat gtctttgact ctataaataa
     7441 aaacatagca gatttaaagc aacatcttga acttactaat gtaaatataa aagatgtatc
     7501 agtgcaaatg cacagcgata atacaaatac atctgatacc tttgataaag catttgaaca
     7561 acagaataga caaaataacc aagaaaacat gagtaagaag aatacaaatg ataagcataa
     7621 taatgtagtc atagaagatg acattaaaga aaatattatt gatgatgata gagtagattt
     7681 actagcttag taagggggaa gtattgcatg tacggaatag caacggcttt ggcaggaact
     7741 cagggaaact ataggtcaaa taacttaagc caacttgaaa gaaatattaa tgatattgcc
     7801 aataaacaag ttagaagcaa aggaaatcag acagtaacag acaatgggac tccaattata
     7861 ggacaaggtg atgatgctga taaagacttg tttatgaaga tacttgtggc acagatgggt
     7921 aatcaagacc caatgaatcc gcaagaccca acagaatatg tgtcacaatt agcacagttt
     7981 gcaactttag aaaagatgtc agctatgaat gagaagctag atgtactttt agccttaagt
     8041 aattccactc taataaactc tgctttgagt atggcaacat cattaattgg taaagaagta
     8101 gaagtatctg gggaagagaa agaagatgaa aacattgtag gagtagtaaa gtccacatat
     8161 ataaaagatg gggaagtaca cttagaatta gaagtagatg gaagtgacaa aactgtaggt
     8221 gttaaatatg aaaaattact taaaatagga gaggtagatg aaaattcagg agaagaaaat
     8281 gattcttcag aacacgaatc tccagaagaa gcataatatt aaatgagaaa agaggtaaat
     8341 tcatgataaa agcaatgtat tcaggtgtaa gtggaatgaa agcaaatcaa acaaaattag
     8401 atgtaatagg taacaatgta gccaacgctg gaactacttc ttttaagaaa agttcagcta
     8461 gaataacaga ttcattttat caaactgtac tatatgcaag tgctccaaca gctgcattag
     8521 gaggaacaaa cttaggacaa gtaggtgtag gtagtaagat atcaagtata aataaagata
     8581 tggtacaagg aaatgtacaa ccaactaata gaccatctga tttaatgata gatggagatg
     8641 gatatcttcc agtagtaaga caaggaacag ttatgtatac gagagatggt tcttttaatt
     8701 tagatacagg atttaaagca gctgatggtg ctttgaagaa tattaaaggt ggaagacttg
     8761 taaatggtga tggatatatg ttacaaggta tgatttatga tggggaatat aaagctgata
     8821 ctggagaatt tgttaaaaaa ggtgatgcta aaggaaaaga gcctattgta ataccacttc
     8881 aaagtataag tcctaaggat ggaacagttc aaaatgtact agagtataat atatctaagg
     8941 atggaacagt agaattttta ttgagtgatg gtcaaagaac tcaatttgtc tatgatatac
     9001 ctgatggtgc taaggtagga gatacacctg ctggaataga taaattaaaa ggtacaatac
     9061 aaaaagtgca aatatatgca ttccaaaatc cagcaggcct ggatgctgct ggaggaaact
     9121 tgtttaaacc atcagcaaac tcaggaaacc cacaaatagc aggagctaca ggcacaataa
     9181 tacaaggggc aatagaagct tccaatgtag atatagcaga agaatttaca gaaatgataa
     9241 tagcaagtag gtctttccaa gcaaacagta aaataatatc gacatcagac gagatacttc
     9301 aagaaataat aaatcttaag agatagtagg tacatattat gataagagta acggatttaa
     9361 acggaagaaa atacatatta aattcagacc ttatagtgag gattgattct gtaccagaaa
     9421 gtaggattct ccttacaaca ggagaaaaaa accttgtaaa agagtcggta gatgagatag
     9481 tagataaagt aataaagtat aaaagaaaaa taaatgtagg ttatattagg agcgaaaaat
     9541 aatgaaaaaa acagattcac tgacatttat tggactaata gtatctacag tattagtttt
     9601 agttggtgcc gcaaaaggaa gtagttcagg gttaaagaat ttttttgatg tttcatcaat
     9661 attaatcaca gtgcttggtt catttggagc tctaatgatt acatttacta tagatgatat
     9721 aaaacttata aaaaatgcat tacaatactc atttaagaca atgagtgtat caaagttaga
     9781 tttactagaa caatttaaaa cactttctaa aaaagctaga aaagaaggtc ttttatcaat
     9841 agagagtgat gtaatgggaa tagaagatcc atttatgaag aaagggttag agctgtgtat
     9901 tgatggattg gaaatcaatg agataaggtc catcttagaa ctagagatag attctgttga
     9961 agatacaaac aacagatact ctaaactatt taaaacttgg ggaacctttg cacctgcttt
    10021 tgggatgttg ggaacactaa taggtttaat acaaatgtta gctgacctta catctccaga
    10081 tttaatagca tctggtatgg gaaaggcatt aattacaacg ttttatggtt ctttacttgc
    10141 caatatcgct ttgaacccaa ttgcatataa tatagatgag aagactgaaa aagaaattta
    10201 tgtaaaggaa atgatgttgg aaggtataat aagcatacaa tctggggaga gcagtatagt
    10261 agttgaggaa cgtttagcca catacctttc aaacaatgag aagttagaaa ttatgaaatc
    10321 caataaaaat acagaaagag caatgagcaa tggcgcgtaa aaaaaataag aaaaaggatg
    10381 agataaatcc agatgcatgg ctggctactt atgcagatac tataacactg attcttacat
    10441 ttttcgtatt attgtatgca acatcagttg tagatatgga aaaatttaaa aacatagcag
    10501 atgcgttaaa aggtcagttt tctgggacaa gtatatttag tacgggttca agtacaagtg
    10561 agaaatctcc aatagacagt gttttagtgg ataaaaatga taaatcaaat ttagaacagg
    10621 atttaaaaga taaagttaat aattctaact tacaaaatag tgtaacagtc aaagatgata
    10681 gtagagggat tttactagag ttagatgatt caattttatt tgactctggg atttctgagt
    10741 taaaagtaga tagtaaagct actcttaata aagtatatga catgataaag atgatgaaaa
    10801 ataagatagt tatagaaggc catactgata atgtacctgt aaaatcaagt acacatgaat
    10861 ccaattggga tttatcttct agtagagcag taagtgttgt aagatacttt gtagaagaaa
    10921 agggcatgga ccctagacta ttttcggcaa caggatgtgg agagtatcaa cctttggttt
    10981 caaatgaaac agcaaaggga agggctgaga atagaagagt taatatactt ataactattg
    11041 actaggagga agaggtttgt tcaaaaataa aaaaattatt ataattattt tgatactgat
    11101 tattttctca gcgggagcag gtttttttat attaggagaa ggtggaaata aacaagttga
    11161 caagattgca ggtttgttta ttccaacagg aaaaggtaaa gatgaagtaa agaccgaaaa
    11221 tcttgactta gaagagatgg tattaaagct agctgatgaa ggtgtaagat atttaaaagt
    11281 atcaataact gtatcttatg attcttcata taaagatata gaaaaaaata cgcctttaat
    11341 tagggataat attataaatt gttttatggc aaaaagggca gatgatttta ctgctgataa
    11401 tttaaagaat ataaaagaag aaattaaaaa tacattaaat atacaactag gtgaaaatgt
    11461 aattcaaaat atatatttca caaatatagt agtgcaataa aaggatggta agacatgata
    11521 ctttatttac taaaaatatt aatatgtatc ccaataattt taatattgat agtcttatct
    11581 ttaaaaatta gccaaggaac tttattaaaa aaatataatg ataaatatgt aaaaatacta
    11641 gatattgtaa atataagtaa aaacaatagc ataataattt taaaaatagg tgaagaagga
    11701 tgcattatgg catcttcttc ttctagcatg agtaaaataa aagatttgac caaagaagaa
    11761 atattaaaaa tagaaaacga cattaaagaa agaaatttta aaattcaaga ttttaaaatg
    11821 gaaagaatta aaaagaaata tgatttcagt agatatattg gaaaatttac aaaaaaggaa
    11881 gataagtaaa tggaactatt atcaaaatta cctttggctg atgtaccata tacaagttca
    11941 atgcaattgt ttttatttct aactgcatta atgtttttac catttgtaat gttcatgatg
    12001 acaagctttg tcagaatagt tataagttta tcatttttga aatcagcatt aggagcacag
    12061 caagcaattc caagtcaaat tttggtagga cttgcaatag tattgacaat atttataatg
    12121 agaccagtat taaatgagat aaacgaaaag gcattacaac catatatgaa agaagaagtt
    12181 actatggaag aagctatgaa ggaagcagaa ggacctatta aagagttttt attaacacaa
    12241 accagacaga cagatttaga tttatttgta gaacaggctg gtttaaaaga aaagaaatta
    12301 actcgtgaaa atataccact atctgttgta gttccagcat ttgctataag cgaactaaaa
    12361 actgcttttc aaataggttt tttaatatat ataccatttt tgataataga ccttgttgtg
    12421 gcaagtgttt tgatgtctat gggtatgttt atgttgcctc cagtcatgat atcattacca
    12481 tttaagttat tattatttgt aatggtagat ggatggaact tgatagtaaa aacattgata
    12541 ttgggatttg gataggagaa aaatagtatg agtgaattta tgataatgag tgttcttaaa
    12601 aatggcatgt ttacaggagc tatgattgca gctccagtac tcattgtttc tcttgtagtt
    12661 gggcttttaa taagtgtgtt tcaagctaca actcaaatcc aggagcaaac tttgactttt
    12721 gtaccaaaac taatagctat tcttttagta gtagctattg gtggaagctg gatgttacat
    12781 actcttatta gctttacttc tgagatattc aatatgatat ctcttataac taaataagta
    12841 ggatacatat gatttttgta tttattagaa tagtagcatt ctttggaaca ctaagaatac
    12901 tatttccagc tggtacacct gctttattta agtcactctt tgcaatcatt atatcaatat
    12961 tgatttcaag tactatgcaa attgaatatg caacaaatat agacaacatt gtattgttta
    13021 ccttatatgg tgtaaatgag accataactg ggattatgct tggatatata accaacctat
    13081 gtttttatag tatcagaatg gcgggttcta tgatggacca acagatggga ttatcaatga
    13141 ttaatatgtt tgacccaaat tcaatgactc agacaacttt aattgataat cttatgaact
    13201 ggacagcact tatgatattt tttagcatgg atggtcatca tgttttaatt agaggtataa
    13261 ggtatagttt tgagttaatt ccaataggaa aaccatttgt agataataac atagattata
    13321 taataaacat atttgtgcag tgctttttaa ctggattcaa aatagcaata cctattgtac
    13381 tttgtctgct tatggcagat tttattttag ggcttatatc aagaagtgta cctcaattaa
    13441 atgtaatgat agttggtatg ccattaaaaa tattggttgg tattgcatta tttatcatat
    13501 caataccttt aattgtcaat caaataagtc atttattaag tcaaatacca aagatgtatg
    13561 agggaacttt tgcactagct cctatgtttt ttatgggttc agcagataaa acagaagaag
    13621 ctactccaaa gaaaaaggga gaacaaagaa agaaaggtaa tatagcaaag agtagagaat
    13681 tacccgttgc aatgactcta cttgcattca ccttattagt acctacatta ttttcatatg
    13741 tagtagatac acttaaatct tcgcttaatt attttttgag tctagatttt tatatgaaca
    13801 taaattattc aaacctagag aaattagtta tagcaggatt aatggatttt tttaagatat
    13861 ttttaccaat agctattccc tttttagttt taggtataat agcaaaccta tttcaggtgg
    13921 gaatactgtt tactggtgaa actttaaaac caaatttatc aaaattaaat ccagttagtg
    13981 gattcaaaaa catgttttct atgagaagtt tgtcaacctt aataaaggat acagcgatta
    14041 tatctatttt ggcttacata ggatatacat tcttccaaga taattattta gatatattaa
    14101 agttaggtaa catataccta ccaactttaa tgtacacagt taaagactta gtatattcaa
    14161 tattgagtaa gatttgtgta gctatgatag ctatagcagt tgcagattat gtttatcaaa
    14221 gatacagtca taaaaagcaa ctgagaatga caaaacaaga agttaaagat gaatataaaa
    14281 actcagaagg agacccagaa gtaaaagcta agataaaaca aaaacaaaga caaatttcgt
    14341 ctcaaaggac aatgcaagca gtaccaagtg ctactgtaat agtaaccaat ccaacccact
    14401 tatctatagc agtgagatat gaaaaaggga aagaccaagc tccagtagtg gtggcaaaag
    14461 gtgcagatta tttagctttt aaaataaggg aaattgcaaa gggaaatgac atccctataa
    14521 tagaaaacaa acctattgct aggttgttat ataagcaagt agaaatagac caagaaattc
    14581 cagaggacat gtatcaagct tttgcagaga tattagtggc agtatataaa atcaaaaata
    14641 gatacaaagt ccccaaaagg taggataagt aatgaataag tttaatttaa aggaaaatat
    14701 ggatgtaata gtggcatttg gtgttgtagg gattgttctt atgatgatag tgccattacc
    14761 tacatttata ttagatattt tactggcaat taacataagt ttatctgtgc taattttact
    14821 aatgacatta tttacaacaa atgtacttga tttatctata tttccaacag tgttacttgt
    14881 gacaacacta tttagattgg gattaaatct ttcatcaact agattgattt taacacaggg
    14941 atatgcaggt gaagttatag aggcatttgg aagttttgtt gtaggtggaa attacgtagt
    15001 aggaataatt atattcttaa ttatattaat tgttcaattt gttgttataa ccaatggttc
    15061 tggaagagta tctgaagtaa cagctagatt tacgttagat gccatgccag gtaagcagat
    15121 gagtatagat gcagatttaa atgcaggagt tatagatgac aaaactgcta gaaaaagaag
    15181 aaaagagctt caacaagaag cagattttta tggttctatg gatggagcaa ataagtttgt
    15241 aaagggagat gccatagcag gtcttatagt aacagcaata aacataatag gtggaattgt
    15301 aataggagct gtaatgctga atatgactct tatgggtgca gcgcagacat atgtaagact
    15361 tactataggg gatggtcttg taagtcagat acctgctcta ttaatatcta catcagcagg
    15421 tataatcgta acaagagctt ctactgatga aaactttggt ggattagtag gtaaacagtt
    15481 aacggcaata ccaaaagcag taatgatgac tggttgtgtt ctaatcgtac ttggtatgat
    15541 gcctggatta cctaaaccag catttttttc actaggtata ggagctatag cagcaggtta
    15601 ctttttagga agagaaaaga aagatacttt agaagacttg gagtttgaaa aacctgatat
    15661 ggtaaaccca gataaaaatg aagtagaaga tgtaacaaac ttaataaatg tagaatcaat
    15721 agaagtagaa attggatatg gtttaatatc tttagctgat gaatcagcag gaggagattt
    15781 acttcagaga atagcatcaa taagaagaca atgtgcaata gacatgggta tagtcgtaca
    15841 acccataaga ataagagata acttacagtt aaatccaaac caatacaata taaaaataaa
    15901 aggaaatgta gttggtgggt atgagataat gcctaatatg gttttatgta tggatcctat
    15961 gaatcaagga tttagtatat ttggaataaa gacaattgaa cctacttttg gaatagaggc
    16021 actttggata gaaagtgaca aggtagaaga tgcagagtta aagggttata cagttgtaga
    16081 ttcatccaca atacttataa ctcacttact agatataata aaatcaaatg cacacgaatt
    16141 actgggaaga caagaagtta agactattat agatgcagca agagaaaatt atagtgctgt
    16201 agtagatgaa ttgataccag atttgatgac acttggtgaa attcaaaaag tacttaaaaa
    16261 tctgttaaga gaaaaagtaa atataaagga tagagttaca atacttgaaa cgcttgcaga
    16321 ccagtctaga aatacaaaag atatagagct tcttactgaa tatgtaagaa tagccttggc
    16381 tagaagtatt tgtaataatt tagttgatga tgataagaca attgtagtgg ctacattgtc
    16441 tccagaaact gaagagttgg tatctaataa tttacagagg tctgtaaatg gaacttatcc
    16501 agcagttgac ccagaaaata caaataaaat atttgaaagt atacaagaag taatgagcaa
    16561 tgtatatttc aatcataata taccagtaat agtagtatca ccaaagataa gagctccatt
    16621 tagaaaatta gttgaaattg tatatcctaa tttgactgta ttatcattaa atgaaatacc
    16681 taacgatatt aaaatcaaag cagaaggtgt ggtgagcata tatgatgacg aaaaagtata
    16741 cagctaacac aattcaggat gctatgaatc tagccaaaat ggaattgggt gacaatatta
    16801 cattaataga taaaaaagag gtaagaaagt ctggaataca aggtatcttt tcaaaaaaag
    16861 atattgaact aactattgga tgggaaaaga gagaaaatgt agaacaaaaa gatttaaaac
    16921 gagagataga acaattaaag tcaattatta acaatatggg gtttgataat aagaatgata
    16981 atgatataga taaaatatgt aaaaatctac taagtttaga attgaatgaa gaaattgtag
    17041 aatctataag aattgatttg caagaaatga agttcaatgg aatcgataca agtaaaaatt
    17101 tagtagaaat tttaaagaaa aagattaaaa tagaaaatca agtaataaat ggtaaaattg
    17161 ctctagtcgg accaccagga gtaggtaaaa cgactacaat agcaaagtta gccgcaaaat
    17221 tagtttttga agaaaataaa aaagtaggag taataactat agatacatac aggattggtg
    17281 ctgtagaaca gttgaagata tatactgata taatgaatat tccctttaaa ggggtaataa
    17341 gtcctgatga aatggaattg gcacttgacg agatgaaaga ttgtgatgtt gtattaatag
    17401 atacaacagg tagaggctac aaaaactcta tgcaaatact agaaatcaaa aatcttatag
    17461 ataaagctga aactgataat atacatttag ttttgaattg tacaacaaga gaaagtgata
    17521 caaaagcaat tatagattca tatagaaatg tgaattttaa aagcttaata attacaaagc
    17581 tagatgaaac aataacatat ggctcaatat ttaacataat gaattatgcg caaaaaccaa
    17641 tatcatatat tacaacagga caaaatgttc ctgatgatat tataaaacct aatgaagata
    17701 agattataag gttactatta ggagttgaaa gtatatgata gaccaagctc aaattttgag
    17761 aaaaatagca atggaaaaaa gaggacttga tgagtttata gtagagaatg agaatgtacc
    17821 taagataatt acaatagctt ctggtaaagg cggtgttgga aagagtaatc tcgctacaaa
    17881 tttgtctata tgcttgacca aacttaataa aaaagttctt atattagatg cagacatagg
    17941 aatgtctaat atagatataa taatgggggt aaatgtaaaa gggactataa ttgatgtaat
    18001 taatggcgaa aaaaatatag aagatataat ttcacagact aagtatggtg taaatataat
    18061 atctggaggt tctgccctta atcatataga agactttaca gaagctcaaa gaaataagtt
    18121 tatacatagc atagaacaaa ttcacgatgt ggattttata ataattgata caggagctgg
    18181 tatgagcaaa agcctattgt catttatata ctgtagtact gaatttttct taataacaac
    18241 tccagaaccg acatcattga ctgacgcata tagtttatta aaagctataa gtaattttgg
    18301 cataaagaga agtgcaaata taataatcaa tagggtaatt gatttagaag aagcaaaatt
    18361 tacatacaaa agaataaaaa tggttgtgga caaatttcta aaccttaatc tagaattgta
    18421 tggatatata atagatgaca aaaaagtaag tttatgtgta aaaaaacaga taccattcat
    18481 tttagaatat cctacaagta ttgcatcaaa atgtattata gccatagcaa agagaatggt
    18541 aagtcagtca agagaagata ttgttgataa taaaagtttt ctaagaaaga tgtttagtat
    18601 atttaaaagc taaggaggcg tagttaatga atagagaaga attaataaaa gagaatatgc
    18661 ctcttgtaaa gagtatagca agaaaatttt ttataccagg gaaaggtttt gaatatcaag
    18721 acttagtaaa tagtggagtc attggattga ttgatgcaat aaataaattt gatgctgaaa
    18781 aaggggcaaa gttttctagt tactcttata taaaaataaa atctgctata ttagatgaaa
    18841 ttagaaatca aagtccaatt tctaaacata atctaactaa agtaaataag tataatagag
    18901 tggtagaaaa gttgcaatct aaattattaa gagagccaac ttcatatgaa attgcaaaag
    18961 aattaaaggt ttcagaaaaa gaacttcatg atatcgaaag taatatagat atgttaaata
    19021 ttgtatcatt aaactatgta atctttgaag atacaaatga aactgtacaa gatgtaataa
    19081 gtgatagaga agaggaagct ccagaaaata taatagaaga agaagaaaag ttagagattc
    19141 tatccaaagc aattagtaat ttaaatgaaa gagaaaaatt aatactttca ctgtactatt
    19201 atgaagattt aaatctaaag gagataggga aagtgctagg tgtttcagaa tcaagagttt
    19261 cacaactcca cagaaaatca ataagaaact taagaaacaa gataaaagaa cttaaatatt
    19321 ctatatagat ggtgattgat aagatgatac aaattatatt aatagtagtg tctattttaa
    19381 taatagtaac tatagttata aatttacata aggaatcaaa aaataaagat aacagatact
    19441 ttgataaaat atttgagcaa gaatatgaag caaatgatag attaataatt gcagaaaaaa
    19501 gaagattctt tttagacaaa gtacttgcag aaaaaagaat taatagtcat ataaaagttt
    19561 atgaaaatta taacctaata aaggctaatg aagaaagaaa ggaaaacata tttatagaat
    19621 caaaaaagga agtaagacaa gaaaatgagt tatctcttaa agtgaaacat cttataagtt
    19681 caggttatga tgatgtggaa atatgtaaga tgctagatat tggaaagggg gaactgttat
    19741 taataaggag ttgttacaaa atataaggat attattatgt gataaaaaag tattcttagg
    19801 aataggtatt ggtatattaa tatcaacttt atgtataatg ctttttagta ataatgatat
    19861 gagtaaggca gagatagaaa aaaaggccag agcgttaggc atgaaatata caagtgagat
    19921 gaaagtttta gaatagatga tattgtttag acatttacaa aaaaggaggt cataatgtga
    19981 taagaggatt atatacagca gtttctgcta tgataactaa tcaacaaaag caaaatgttg
    20041 ttgtcaataa cctagctaat atggatacaa atggatataa aagtaaacaa cttttgacta
    20101 aaagttttga tgaattaaag ttggagggtt acaataacta cgcaaatgga actaaacaaa
    20161 agcagattat aggcgatata agtccaggta tttctatgga tgaaactatc acaaatttta
    20221 atcaaggacc tataaaaact actgataata aaatggatgt agctattcaa ggtaagggat
    20281 tttttctagt tagtgatgca gctggaaatc aattttacac tagaaatggg aacttcagaa
    20341 cagacaatca aggtgacctt ataacaagtg aagggtatta tgtacttggg acgaatactg
    20401 ctactggtgc tgtaggtcat ataaatgtag gaaatcaaaa atttgaagtg gcaaaagata
    20461 atacaatatc actagacgga aatgctatgt ataaatttaa tattgtagat tttaataact
    20521 acaataactt aaaaaaagaa ggagataact tatattctgg aggaggagct atgaaagtaa
    20581 ataatgcact tacaatacaa agtgcgatag agtcctctaa tgtagatatg gtatctgaaa
    20641 ttaataatat gatgacaata tcaagagaat atgaggcaaa tcaaaagata attcaggcta
    20701 tggattctaa attagcaaaa atagcaagtg agattggttc agtaagatag gtaataggag
    20761 gtgaattttt atgtacacaa tgttttatac tagtaaaaca gctttatcag caaatcaaag
    20821 taaactagat ataatatcta acaatatagc aaattctgat tcaacaggat ataaaaaaat
    20881 aaatatggaa tattctgatt tagtggatga agtactaaat agaccatcat atcctacaaa
    20941 tggtaaagat atatcgactg gaacaggagc taaggcaaca gctcccatga gaaattatga
    21001 acaaggtgca ttggctccta cagatagtaa gagtaatttg gcgatagatg gagaaggatt
    21061 ctttagagtg ataagaaaag atggtacgta tgcatataca agaaatggtg gatttaatgt
    21121 agatgcttta ggtaagattg tagatgataa tggaaatata ttagatgtcc aatttgatgc
    21181 aggatataac tataataata caaatataga tagtacaaac ctgacagttt caagaaatgg
    21241 agagctattc gtagataata aaaaaatagg aaaaattaac ttatatcaac ctataggaac
    21301 tcaaaatttt atatctgaag gagatagttt atttgtcgca actgatgcag cacagattaa
    21361 acaagttgag aaagtcgata tagttcaagg ctatagagag aggtcaaatg tttctttaca
    21421 agaagaattt gttgacttaa tagccactca aagagcattt caaatgaact caaagggaat
    21481 aaagacagca gatgaaatgt ggcaaatagc gaataattta agagctaaat aattatgtaa
    21541 ggtgaggaat attcatgaat gatatgaaag agtacgattt tggcaaacct cagagttatt
    21601 caagagaaaa tttaacttgt ttaaattttt tattagctga attttgtaaa aaatataaaa
    21661 actatataat ttatgaactt aagtgtaatt ctagtatggt tgttgatagt atagaccaag
    21721 taaattatca gactttttta gatagagtaa cttcttcatg cgtagtagta caaaatgcaa
    21781 tggaacctgt tatgaaaaac ttttctttta gaatagatag aagtgtagct gatatgtgga
    21841 ttgatataac ttctggagga actggagttg taagagataa tgatagagag cttacagaac
    21901 ttgataaaaa agtgctttta caccttatgg atgatttagt aagaaatatg cacttatttg
    21961 agggttttga aaatatacaa gtattggata cacatacaca tattaattta ccacaattat
    22021 gttctccaac tgcaccagta tgcgttgtag atttaaaagt gctaaatgat aatgagtata
    22081 ttggaagtgt ttctctttgt tttccataca atggtttaga agctctgtta gaaagtatat
    22141 ctgttataga attttttgat aatgatatag gagatgattc agaggaattt accaaaaaaa
    22201 tatataatag tgtatctaat atagaattat cggttattgc agagatagga aaagtatcaa
    22261 ttaatgttga agacttactt aaactagaag ttggagatgt aatagtaaca aataaaaaaa
    22321 taaatgatta tatagatata tttgtagaaa attcaaaatc atatacagca acaccaggat
    22381 ttatatcaag taaaaaaggt gtaaaaataa atgatgctgt tggaaaagag gtgtagttat
    22441 ggacaatagt aatcttattt cagatgaaga agcaaagatg ctattaaatg ataaagttga
    22501 aagtgaagtt gatggagatg atgagttagc cttagatgtg tctgaagtta atcgaaataa
    22561 tatacaaagg atacttgacc ttaagttaga attatctgtg gttataggaa gaactaagat
    22621 gactctaaga gatattttaa acctacataa aggttctctt attgaactgg atactttagc
    22681 agaccaagat gtggaaatac taatagataa taaagtgttg gcttatggaa aagtagtggt
    22741 ggtagattta aattttggtg taaagataac aagtatagta agtgaagaag atatggtaaa
    22801 atctcttgtt tagagataac atcaattaat acttaaggca tgaggtggta cttattttaa
    22861 acttaaatga tataaaaaaa gatatattaa aactcaataa aaaagactca tgttctctat
    22921 ataatgaatc tattaataat ataaataata attgtatatc gagtggattg aaatttttaa
    22981 aacaagcaag agatttaaac ccagatgatg ttgatatatt aaatctcaca ggtcttgtaa
    23041 atttactaaa atgtaatttt gatgaggctg tagaaagttt ctataagagt tcactttgtg
    23101 aaaaaactgc tctgtgtgaa aagtatataa acatgcttac atctgaggag tttttaattt
    23161 tctttgaaag gtacaatcaa ctcataaggt taataaacag aggaatgtat aaagaagcta
    23221 tacaagggtt taaacttata actgagcaat actgtgattt aatagaacct tatgaattgc
    23281 ttgccttact gtatgacaag gaagaagaat atataaaatt tgatgaatgt ttagaagtat
    23341 tgaagattat tgataaggaa aattatttat taaatcaaga aaataacaca ttagatatgt
    23401 ataatagtgc taatatacaa actaaaagta atataaaagt agatgattat aagaatgagt
    23461 tcttacataa aagtagtagt aaccataaaa ttaatgatta taatgatgta actaaagaaa
    23521 agagttcaag caaaaaccag aaaagttcag attctaataa gaaaaaaagt attaaaaaaa
    23581 ataaatacct atatatagcc ataatattag gaatattatt agtaggacaa agtatatata
    23641 gttcttataa gatgaataat ttaactgata aaatatctaa aaataatgaa accaaaaatg
    23701 aagagtcaat agattctaac ttgactaaga aagaaaataa tatagaagaa aatgaagata
    23761 tgagtaaggc ttctaatata aataagagta ataaaaacat taataaagag gatgatgtaa
    23821 aatctaaaaa aaatactgta gaaaatgaag atgataaaga ttctgactta tttacagagg
    23881 atgaattgat gtctaaggcg aatactttaa aatcagaaaa gaaaactaaa tcttctatac
    23941 aactatataa gaaagttgca gatataggtg aaaataaagg gaatacatct gaagcaactt
    24001 atcaagttgc tattctaagt gaaaagttaa aagactataa aactgctgaa gaatattata
    24061 aaatgtatgt agaaaattac tcagaaaaag atgcttattt tgatgaatct tattataatt
    24121 taggtatgat gtactataac aatggtgatt taaaaaattc taaattgaca ttaaagaagt
    24181 tggttaataa ggtaccaaat agtatgtata ataattcaaa agtaaaagaa attttaaaag
    24241 aagaatagtt aaattagata agaaaaataa attattttat tttaaaataa tatgattata
    24301 atatattgac ataccatttt aaaaaattta aaatattaat tataaaatga ggagggataa
    24361 aaaatggaat ttgaaaaagg gagtatttca attcatacag aaaatatttt tcctataatt
    24421 aaaaaatggt tatactctga taaagacata tttataagag agcttataag taatggatgt
    24481 gatgcagtaa gtaaacataa aagacttgta tcactaggag aaatatcaga aaataagtca
    24541 tcagattata aaattacagt atctgtaaat aaaggagaag gtacacttaa atttattgac
    24601 aatggaattg gtatgactga agaagagata aaaaaataca taaatcaagt tgctttttca
    24661 ggtgctgaag atttctttaa taaatataag gataaaatgg aagagtcaaa tgatataata
    24721 ggtcattttg gattaggatt ttattctgca tttatggtgt ctaaaaaggt acaaatagat
    24781 acactttcat atacagaagg agcgactcct gtaagatgga tatctgaagg tggtacagaa
    24841 tacgaaatat ctgaatcaga tgctagaaat gatagaggaa ctactataac actatttatt
    24901 gatgatgata gtaaagaatt tttagatgaa tttactgtaa gaggaataat aaataaatac
    24961 tgttctttct taccagtaga aatatattta gaagatgttg aaagattaga aagagaagct
    25021 aaagaagcag aagaaaaagc taaaaaacaa aaggaagatg gaaaagaaga gattgtagat
    25081 gcaaaagtaa ttgagccttt aaacgacact aatcctctat ggttaaaatc tccaaaagac
    25141 tgtactgatg aggagtataa agagttttat agaaaggtat tcaatgtatt tgatgaacct
    25201 ttattttgga tacatttaaa tgtagattat cctttcaatt taaagggtat actttacttc
    25261 cctaaattaa aaaatgagtt tgaactgact gaaggaaaag taaaattata caacaatcaa
    25321 gtgtttgtgg ctgacaatat aaaagaagtt ataccagaat ttttactttt attaaaagga
    25381 gttatagact gcccagactt accattaaat gtatcaagaa gtttcttaca aaatgacagg
    25441 gatgtaagca aaatttctaa acatataatt aaaaaagtag ctgataagtt gaaatctctt
    25501 tgtaaaaatg aaagagaaga atacaataag ttttgggatg atatacaaat atttattaaa
    25561 tatggttgct taaaagatga aagtttctat gagaaggtaa aagaatgtat attattcaaa
    25621 actattgatg atgagtatat aacattacaa gattatttag aaaaatgtaa ggacaaacat
    25681 gaaaataagg tattttatgt aagtgataag gaacagcaat ctcaatacat taaactattt
    25741 aaagaatacg acttaagtgc tgttgtttta aactcatcaa tagatactca ttttatttca
    25801 tttatggaat ataaagagaa tggagttaag tttaatagaa tagatgcaga cctttctgat
    25861 gtattaaaag ataaaaatga aaataaagat agtgaagaaa acaaagaaga gatagctaag
    25921 atagaaggtc tgtttaaaga agctgtcgga gaaagagtga agaattattc tgtagaagga
    25981 ttgaaaaatg aagacactcc agctatggta ttagtatcag aacaatctat tagaatggca
    26041 gaaatgcagt caagatttgc tggaatggat ttaggaatga attttgagga agaaaaaact
    26101 cttgtaataa atgagaacag tccaataatc aaaaaattgg tttctcttaa agatgatgag
    26161 gaaaagaaag ataagattac gcttatttgt aaccagatag cagatttagc tttactttct
    26221 aataaagaat taaagccaga tgagttagat tcatttgtac aaagaagtaa taagcttatg
    26281 agtcttttga tagaattata agattgaatt tgaatacatt gaagcaaatt tataaaaagt
    26341 attcaatatc agtgatgaga aagaagtgtc tcaaaatgaa cttttcagtt catgaggcgc
    26401 tttttttatg tgaaattaaa tttattttaa tcatttcaac aatgaaaaat atatttatct
    26461 ctattttgag acagaccgtt ctttattttt ttatctttgg ttaattttaa atatttaagt
    26521 aggttctaat aaaaaactct atttagttaa atatagttaa cttgatatat aataagataa
    26581 tacatataat taagtaaaag attgtttttt agtttatgtt taaatacata attacacttt
    26641 gttaaaaaca taaaattatt actaaagtag aaataggagg ttttagaatg aattttaatt
    26701 ataatttgcc agtaaatatc ttatttggga gaggcagaat taatgatgtg ggaaaagaag
    26761 tatctaaata tggcaaaaaa gtattgatag taacaggtaa aaatagtaca aagaaaactg
    26821 gattacttga taaaacaata gatttactaa aagactctaa aatagaatat gaagtttttg
    26881 atagagtaga acaaaatcct cttataacta ctatatattc aggagtagaa attataaaat
    26941 caactggatg tgattgtgtt ttagggcttg gtggtggaag cattatggat gctgccaaga
    27001 gtatagcttt ttcttataaa aacccaggag atttaaatga ttatatattt ggaattagac
    27061 aaggtgaaga agctttacct ataatcttag ttccaacaac atcaggtaca ggtagtgaag
    27121 gaaattgttt tgcagtactt acaaattcag aaactaaaga taaaaaatct ataaagaaga
    27181 atagtatgta tgcaaaagca tcaataattg acccagaact tatggttaca atgccaaaac
    27241 atataattgc atcagttgga tttgatgcac ttgcccataa tatggaagca tatctttcta
    27301 atggaagaaa ccctcttgca gatgtacaag ctatttatgg gattgaactt atatcagaaa
    27361 acctaataaa ggtatataat gatgttaatg atttagaagc atgggaaaaa ataacacttg
    27421 caagtactat tggaggaatg acgattggta cagcgggtac agccttgcca catggaatgg
    27481 agcatcctgt aagtggtctt agagatgtaa tgcatggaaa aggacttgca gctttgactc
    27541 ctactattat agaaagttca tgggatagtg atattgaaag atttggtaat atttcaaagg
    27601 tattaggagg aacttgtgca gaagattgtt cagatgcaat tagaaatttc cttaaaaaaa
    27661 tagatttaga tataaaacta agtgatttag gtattgaaaa atcagatata gattggttaa
    27721 ctgataattg tatgaaaata tcagttgcaa atattaagag acatccaaag tattttaata
    27781 aggaacaaat aaaggaaatg taccataagt ctttataatt tgactttaca tatttagttt
    27841 tatataaaaa ttataaatta ataataaaaa attataagta attaatagta aaaaaattgt
    27901 gtatagaagt tatatatata ataaacttgt cttaagcttt tattttactt tctaataaat
    27961 aaaggagttt aaaaaaggga agattaatta agtaatattt tataaaattc ttcgatttaa
    28021 aaactttaag tagaggtgaa ataaatgttt aatcctaaaa gaataatatt cgaaaaagga
    28081 actttagaca ctgatgtggg taaaaaaata tacaataaat ttagagataa taaagatgta
    28141 gagataatta atatatcatc aaataggatt aaagaacata ttccaggtga aaatctatat
    28201 gaacaatata gaagtgggaa gcagactatg gtagttggga ttagaaaaag tctgaagttt
    28261 caaacatgta aaccttctgc taattatcaa ctaccacttg tcagtggatg tatgggaaga
    28321 tgtgagtatt gttatttgaa cacacaatta ggtgataaac catttataag ggtgtttgta
    28381 aatgtagatg aaatacttga acagacaaaa aaatatattg aagatagaaa gccacaggta
    28441 accatatttg aaggggcagc tacatcagac ccagttccag tagagccata tactaattct
    28501 ttgaagaaaa caatagagtt ttttggaaga gaggaaaaag gaagatttag atttgttact
    28561 aaatacagtg atgtggatag cttattggat gctgaacata ataatcatac agaaatacga
    28621 tttagtatca atactcctag aattataaat gaacatgagc attatacagc ctctgcaaat
    28681 aaaagaatag agtcagctgt aaaacttgct agggctggtt atcagattgg tttcataata
    28741 gcacctgtgt ttttatatga aggttggaaa gaagagtata aaaatctgat tataagtata
    28801 aaagaagctt taccaaagga ttttgataaa aaaattatat ttgaagttat atctcataga
    28861 tatacaacta aagcaaaaaa tagaatacta gagatttatc cagatacact attgccaatg
    28921 gaaaatgaag atagaaagtt taaatacggt caatttggat atggtaagta cgtgtatgat
    28981 aaagaacaat tagatgatat gaaagagttc tttcttaagg aaataaatgg agtatttaaa
    29041 aatagtttaa taaaatatat aatatagcaa atcttaccta aagatttgct atattttatt
    29101 tttatatatt ttatgtttat aataaagcaa tgagatttag cactatcata taagaaaatt
    29161 aaaaagggca ctatctatca agaacatttt ttctagtttt gatactataa aggataagag
    29221 taacaaaaat atcaggagga gttatagtat gcatgcatgg gagtctattc aaattacatt
    29281 ggatttaata gaagcaaatt tatcagaaga aatatcaatt aatgaattag caaataaagc
    29341 aaatttatca ccattttact atcaaagact atttaaaaga ttggtaaata agactgtaat
    29401 ggagtatata aagcttcgtc gtcttgcaag agcttctgaa tacttagcag aacataagaa
    29461 tcgtatactt gatgtagctt taaattttgg atttgctagt catgagacat ttacacgttc
    29521 ctttaaaaaa gcatatggat taactccaga aaaatataga gcaaatccag taaaactcaa
    29581 tcactttatt aagccagaat taattttaaa ttatgctatg gttgatgaag gtgttccact
    29641 gattgcagat gatatagtga ttgaagttaa tcgtaagaca ttgagtaatc caaggacatt
    29701 tgttggaata gaaatagaag tacctatttg ccagttggta ggcggtgaaa caactggaat
    29761 tgcaatagta gaggaattat ggatgaaact tggaagtcaa agacataata taccaaatca
    29821 aattccaggt ggaaatgaat ttgctgctct ttatatggga aatgcaaaag aaggaaattg
    29881 tatgtatatg gcaggtgtag aggtagagtc aggtacatca gtagaaggat atagtacttt
    29941 tgagttgcca gttaaagaat atttagtatg tggatttgag gcagaaaact ttaatgagtt
    30001 agttaattca gctgttttta aagcagataa atttatggaa agatggatga agaaacataa
    30061 tttaacaact actgattttg caattgagat gtattacccc actacacctg aagcagcata
    30121 tctggaacat tggattgttc cggttcctat tgaacaatag aaaggatgat gaatatgaat
    30181 tggcaagatg cgttttttca agatactaaa cctacatttg agcaaattaa tgaatttatt
    30241 aataatccat tatgggacaa gttaaataaa actttggttt ctacttataa tgtagaacct
    30301 aagcttgaat acagtaaatg ttcaatgcaa agaggttgga atgtaaaata taaaaaaaga
    30361 ggtaaatcac tatgtacttt atatccacaa gaaggttatt ttaaagcttt ggttattgta
    30421 agtgaaagca atagagtaga ggttgattta tttattaata cttgttgtga ttatatcaaa
    30481 aaaatatata atgaggtaaa cttttttaat ggaagtaaat ggctaatgat tcaagtagat
    30541 agtttattgg tattaaacga tatgcttgaa ctaattaaat ttagagctta acatatagaa
    30601 caggatgaat atataagtca tcctgttttt atattcaatc agataaatta atatatagtc
    30661 tataatatga ataatttagt aaatttaaat ttactttatc tttctacatt ctttgcaact
    30721 aagtccacat gtgttttttt cttgcatatt agtagcaccc caattagcaa attgttgtaa
    30781 aataggcaca atacttttac ctttacttgt taatgtatat tcaacttttg gaggaatttc
    30841 attatattgt ttacgatgaa caataccagt agactctaaa tcttttaatg attgagctag
    30901 catagtattt gtaatgccat ccaattttct ttttatgcta ctatatctaa ttggattttc
    30961 aactgctaac atacatataa taggtaattt ccacttacca ccgacaatgt ctaatgcata
    31021 acggattgga caggttaatt cttcaacgtt tttagttata tttttttcag tatttatact
    31081 cattttaatt actccttagt tatagtagta tcttttttat actttatcag aaaaaactgc
    31141 atacttgttt tttctgtatt ttatattaaa attatatcaa aggataatcc ttaatacaag
    31201 tatttaaaag gggtgtaaga attatgaaag cattagaatt tttgaacaag gcaggagttt
    31261 attatttagc aacagttgat gagaacaatc aagcacatgt gcgtccttta ggatttgtta
    31321 tggaatacaa tggtaaactt acattttgca catctaatca aaaggatatg tttaagcaac
    31381 ttatttttaa tccaaaggtt gaactttgtt gtatagacca aaaccttaat actctacgta
    31441 ttttaggtga agcagtattt gttacatcaa aagaaacaca agctaaggca cttgaaataa
    31501 tgccaagttt aggtcagatg tactctgtag gagatggtaa gtttgaaata ttttctattg
    31561 ataaggcaaa agttagttgt tgttcaatga gtggtaaaaa gattgatgta gagctttaat
    31621 tataattgaa tcgacatcca tagtgaaaat taagaaaacg ttattactga aatattgata
    31681 gatttaattt aaatgcaata aaaagatttt taaataatga atatgtaaaa atttttagtt
    31741 attagatgtg tatttcaata atgatatact attttgaata attacatac
//
LOCUS       DAFKMX010000021        31762 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFKMX010000021 DAFKMX010000000
VERSION     DAFKMX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.021"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            273..617
                     /locus_tag="LZB08_000853"
     CDS             273..617
                     /locus_tag="LZB08_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBM7314368.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            648..2249
                     /locus_tag="LZB08_000854"
     CDS             648..2249
                     /locus_tag="LZB08_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBM7314369.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENMKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2268..3368
                     /gene="dprA"
                     /locus_tag="LZB08_000855"
     CDS             2268..3368
                     /gene="dprA"
                     /locus_tag="LZB08_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBM7314370.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3401..5488
                     /gene="topA"
                     /locus_tag="LZB08_000856"
     CDS             3401..5488
                     /gene="topA"
                     /locus_tag="LZB08_000856"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBM7314371.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5653..6438
                     /gene="codY"
                     /locus_tag="LZB08_000857"
     CDS             5653..6438
                     /gene="codY"
                     /locus_tag="LZB08_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBM7314372.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6545..7810
                     /locus_tag="LZB08_000858"
     CDS             6545..7810
                     /locus_tag="LZB08_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBM7314373.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7841..8611
                     /locus_tag="LZB08_000859"
     CDS             7841..8611
                     /locus_tag="LZB08_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7314374.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8720..9166
                     /locus_tag="LZB08_000860"
     CDS             8720..9166
                     /locus_tag="LZB08_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBM7314375.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9184..10377
                     /gene="nifS"
                     /locus_tag="LZB08_000861"
     CDS             9184..10377
                     /gene="nifS"
                     /locus_tag="LZB08_000861"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBM7314376.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEESIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10431..10871
                     /gene="nifU"
                     /locus_tag="LZB08_000862"
     CDS             10431..10871
                     /gene="nifU"
                     /locus_tag="LZB08_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBM7314377.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11160..12239
                     /gene="mnmA"
                     /locus_tag="LZB08_000863"
     CDS             11160..12239
                     /gene="mnmA"
                     /locus_tag="LZB08_000863"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBM7314378.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12382..12620
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12688..15327
                     /gene="alaS"
                     /locus_tag="LZB08_000864"
     CDS             12688..15327
                     /gene="alaS"
                     /locus_tag="LZB08_000864"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBM7314379.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15352..15609
                     /locus_tag="LZB08_000865"
     CDS             15352..15609
                     /locus_tag="LZB08_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBM7314380.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15619..17790
                     /locus_tag="LZB08_000866"
     CDS             15619..17790
                     /locus_tag="LZB08_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314381.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            17996..18421
                     /gene="ruvX"
                     /locus_tag="LZB08_000867"
     CDS             17996..18421
                     /gene="ruvX"
                     /locus_tag="LZB08_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBM7314382.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18499..18786
                     /locus_tag="LZB08_000868"
     CDS             18499..18786
                     /locus_tag="LZB08_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBM7314383.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19068..19526
                     /locus_tag="LZB08_000869"
     CDS             19068..19526
                     /locus_tag="LZB08_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBM7314384.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19643..20380)
                     /locus_tag="LZB08_000870"
     CDS             complement(19643..20380)
                     /locus_tag="LZB08_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBM7314385.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20597..22273
                     /locus_tag="LZB08_000871"
     CDS             20597..22273
                     /locus_tag="LZB08_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBM7314386.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22479..22679
                     /locus_tag="LZB08_000872"
     CDS             22479..22679
                     /locus_tag="LZB08_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBM7314387.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22733..23896
                     /locus_tag="LZB08_000873"
     CDS             22733..23896
                     /locus_tag="LZB08_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBM7314388.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24107..24529)
                     /locus_tag="LZB08_000874"
     CDS             complement(24107..24529)
                     /locus_tag="LZB08_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBM7314389.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24550..25386)
                     /locus_tag="LZB08_000875"
     CDS             complement(24550..25386)
                     /locus_tag="LZB08_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7314390.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25684..26280
                     /locus_tag="LZB08_000876"
     CDS             25684..26280
                     /locus_tag="LZB08_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBM7314391.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            26310..27029
                     /locus_tag="LZB08_000877"
     CDS             26310..27029
                     /locus_tag="LZB08_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBM7314392.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27031..27570
                     /gene="scpB"
                     /locus_tag="LZB08_000878"
     CDS             27031..27570
                     /gene="scpB"
                     /locus_tag="LZB08_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBM7314393.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTSE"
     gene            27732..28361
                     /locus_tag="LZB08_000879"
     CDS             27732..28361
                     /locus_tag="LZB08_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBM7314394.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28315..28743
                     /gene="ytfJ"
                     /locus_tag="LZB08_000880"
     CDS             28315..28743
                     /gene="ytfJ"
                     /locus_tag="LZB08_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBM7314395.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            28991..29431
                     /locus_tag="LZB08_000881"
     CDS             28991..29431
                     /locus_tag="LZB08_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7314396.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            29447..30898
                     /locus_tag="LZB08_000882"
     CDS             29447..30898
                     /locus_tag="LZB08_000882"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBM7314397.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(30989..31657)
                     /locus_tag="LZB08_000883"
     CDS             complement(30989..31657)
                     /locus_tag="LZB08_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBM7314398.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT        12925 a         3268 c         5676 g         9893 t
ORIGIN      
        1 aatgtatttt caacattttt caaatagctt acaactaata taaataaaga taaatatcgt
       61 ataaaagcaa ctaatttttc tctgttcatt aaaggcactc ctccaaaaaa ataaactttt
      121 cctatgaaaa ctagaataat atcataaaag aataaagtca ataaaagtat ttattaatat
      181 tgatggaaat aaaatcaatc caaaaacttg aatagtaaaa ttgtatactg accataattt
      241 aaaagtgaaa atttaatgta ttggagtgaa taatgaataa taaagaaaaa ggtgattttg
      301 gagaaaaagt agcagttaat tatttgctat caaaaggagc aaagatacta gaaaaaaatt
      361 acagattaaa gataggggaa atagatataa tagcaaaaat ggaagatgaa attatatttg
      421 tggaggtgaa atcaaggtct aacataaagt ttggctaccc ttgtgaatct gtaagcttca
      481 aaaaaagaaa aaagataatt ggagttgcaa attattatat tataaaaaat aacttaaata
      541 acactcccat aagatttgat gttatagagg tatatttatt agaaaaaaga ataaatcaca
      601 taatgaatgc tttttaaaaa agataatgtt tttggaaagg attaaatatg ttaagtatta
      661 ttaattcaag caacttagta ggaatcgaca gctttttagt taaggttgaa gtagatgtaa
      721 gcaatggaat acctagtttt aatattgttg gtcttccagg caaagaaatt aaagaagcaa
      781 gagaaagagt aaagtcagca attcttaata gtggatataa atttccaagc acgagaatag
      841 ttgtaaattt atcaccagca gatataaaaa aagaaggagc ttttttagat ttatctataa
      901 gtataggcct tttaagagag ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt
      961 ttataggaga attatcatta gatgggaaaa taagaaaagt aagaggtata ttgcccataa
     1021 ttatgggtgc aaaaactcag aacataaaac gtatttttat accaattgaa aatatgaaag
     1081 aaagtctatt tgtagatgaa attgatataa ttcctattaa aagtttaaaa gaatgtgtcg
     1141 attttttaaa cgaagagata aaagtcgata aagtaaggat aatgtctttt ttggatgata
     1201 agtcaagaaa agagaacgaa gaattagaaa aagataatag ttatatagat tgcaaatata
     1261 ctaagattaa taatgaagaa agtaaatatg atgaagactt taaagatgta aaaggaaatt
     1321 attttgtaaa gaggagtgca gaaattgctg ctgctggaaa tcataatatg tttatgattg
     1381 gacctccagg ctcaggaaaa actatgattg caaaaagagt aaggacaatt ttaccagata
     1441 taagtgtaga agaaatgata gaagtaagta aggtctatag tatattgggt atgataaatg
     1501 aaactaaagg aataattgat aaaagacctt ttagagcacc tcatcataca actacaaagc
     1561 aatctttaat tggaggagga atggatgcaa gacctggaga aattgcttta gcacatagag
     1621 gtatcttatt tttagatgag atagccgaat ttgatagaaa aatacttgag actttaagac
     1681 aacctataga agatggatat gtaaatatat ccagagtaaa gtacagtgca aaatatcctt
     1741 gtagagtact gttagttgca gctatgaatc catgtccatg tggttattat atgtcagaga
     1801 cagagtgtag atgtagaagt aacgaaattg atagatacat aaataaaata tcaggtccat
     1861 tgttagatag gtttgatatt tttgtagaag ttaattctat aaaatattca gattttaatt
     1921 ctttaaaaca agaagaaagc tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa
     1981 tacagataaa tagatttaag aaggataata taaaaaataa tagtgaaatt aaagcttaca
     2041 atttatttaa atattgtaaa ctggaaaaag aagcttcaaa aacggctgag atgatattta
     2101 ataaatacaa cctaagcagt agaagttata caaaactttt aaaaatggca agaacaatag
     2161 cagatttaga agaaagagac ttgataaatt cacagtgtat tatagaagct ttttcattta
     2221 gaaaggctta ttatagttat tttaaatagt tttttgaggt ggattttatg gaaaaaagag
     2281 atacttattt atggttaaaa tcaattggtg gaataacaac aaaaacaatt gaaataatag
     2341 aaaatgaaat agtaaatatt gaagatatat ttgatttttc tgaaaaagaa atatataatt
     2401 taaaaaatat aagcttgaat attagaaaaa atatagtaaa atatagaggt cacgcttatt
     2461 tagaaaatat aaaagaacta ctctacaaaa aagcgattaa atatatttgt aaatatgata
     2521 aagaatatcc tgaaaattta aagaatatat acaacgctcc aaagttatta ttctacaaag
     2581 gtgacatagg cttagttaat aataatttta atatagctat agtaggttca agaaaaccta
     2641 cagcctatgg aattaattgt gcaaaaacaa taagttgtca actatcccaa tatggagtta
     2701 atatagtaag tggattagct ataggaatag atgcatactc gcacataggt tgtatgagtg
     2761 gaaaatctaa aactatagca gtacttggtt caggagtaga caatcctctt ccaaaacaaa
     2821 atttacattt atcaaataaa atattagaaa atgggggatt attattatca gagtataata
     2881 ttaactcgac agtagccccg taccattttt ctaatagaaa tagaattatc agtggtttaa
     2941 gtgatggagt agttgttgta gaagcagcaa taaaaagtgg tgcactaatc acagtagatt
     3001 ttgctcttga acatggaaaa aatgtttttg caatacctgg taatataaat tctcaaatga
     3061 gtagaggttg tcataaaatt ataaaagaag gtgcaaaatt aattgaaaat atagatgata
     3121 ttttaaatga atataacata tttaatatta ttgataaaaa aataaatcaa aaatatgata
     3181 atataagtct aaatgccaag tctaagcaaa taattgaagc tataaaaaga gaaggaaact
     3241 tgcatataga tagtatttgt gattatactg gtatagaaat aaagtatgta aattcaataa
     3301 tcaatgaatt agtactaaat gagttagtag tagaaatgaa taataaaacg tacagtttaa
     3361 atgtataaat taagattagg tagtgggggt gtatggtttt atggccaaaa cattagtcat
     3421 cgtagaatcg cctgcaaaag ctaaaactat agaaaaattt ttagggaaaa gtcattacac
     3481 agttaaagca tcagttggtc atgtcagaga tttacctaaa agtaaattag gtgtagatat
     3541 agaaaataat tttgaacctc aatatataaa tataagagga aaaggcgatg taataaaaga
     3601 attaaaaaaa gaagctaaaa aatctaaaca agtatatcta gcaactgacc ctgatagaga
     3661 aggtgaagct atatcatggc atttagctca tatactaaat ttagatgaat ctgaggattg
     3721 tagaatagag tttaatgaga taactaagga tgcaataaaa aaagcaatta aacatcctag
     3781 aaatattgat ataaatttgg ttgatgcaca acaggcaaga agagtgctag acagactttt
     3841 aggttatcaa ataagtccta tattatggca aaaggttaga aaaggtctta gtgcaggtag
     3901 agtgcaatct gttacaacaa agttaatttg tgacagagaa aaagaaataa aggcatttat
     3961 tccaaaagaa tactggacaa tagatgttga ggctaagaca gtaaaagaca aagatataac
     4021 tttgaaattt tatggtaaaa atgatgaaaa gattgaatta gaaaatgaag aagctgtaaa
     4081 tgaaatacta aaagaaatgg aaggtaaaga tttaaaggtt gagaagatag aatctaaatc
     4141 aagacgtaaa tcagctccaa aaccatttac aacaagtatg cttcaacaag aaggagcaaa
     4201 taagttattt tttactacta aaaaaacaat gatgatagca caagaattat atgaaggtat
     4261 cgacatagaa ggtgaaggta ctgttggtct tatatcatat ataagaactg actctaaaag
     4321 aatatctgat gaagcaaaag aaaaagctaa gggctatatc gtaaacaatt taggagaaaa
     4381 ctactataaa aacactggtg ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc
     4441 ccatgaggca ataagaccaa cctcagttga tagaactcct gagagtataa aagcctcatt
     4501 gagcaaagac cagtacaagt tatacaattt aatttggaga agatttgtag ctagtcaaat
     4561 ggaagattca gtgtttgata tactaaatgt agaatgtaaa ataggcgata ttgtatttaa
     4621 ggcaactggt tcaaaaatga aatttgatgg ttataccaag gtatataatt tttcagaaag
     4681 agaagacaaa attttaccaa gtatagaaaa aggagatata ttaaatatag aaaatatatt
     4741 gccagaccaa cattttacac aaccaccagc aagatataca gaagctagtt tagtaaaaac
     4801 actagaagaa ctaggtatag gtagaccaag tacgtatgct ccaacaatag ctactatact
     4861 gaatagagag tatgttgaaa agcaaggaac aagtctatgt ccaacagaac ttggaataat
     4921 agttactgat attttggaaa ataatttcca taaatttata gatgtagatt ttactgctga
     4981 tatggaaagt aaacttgatg ttatagaaga aggcaataca gattggaaaa agattgtatc
     5041 agaatcatat gcaccactta aagaggctat tgaagaagct cttgaaaata tagaaaaagt
     5101 aaatatggat gaagaaactg atgagatttg cgagaactgt gggtctaata tggttatcaa
     5161 atatggtaga tttggaaagt ttatggcttg taaaaactat cccgattgta aaacaactag
     5221 accaattatg aataaaattg gtgttaaatg tccaaaatgt gaagaaggag atattatact
     5281 tagaaaatct aaaagaggta aagcttttta tgggtgttct aattatccaa actgtgactt
     5341 tgtagcttgg aataagccaa caggagagtt ttgtgagaag tgtggttcat atatggttga
     5401 aaaagtgact aaatctgaaa caaaaacagt gtgttctaat aaagaatgta ataaagagac
     5461 aaaagagcat aatgacggaa aagagtgaaa attgaaaaaa atcacataat tctcttgaaa
     5521 aaggttccaa tctatgataa tctttcaagt gtaaacaact aatagaaaaa taaattttca
     5581 ttaaatgtga aaataatatt agaaaatata aaaggttatt aaaaggccta aatttcatga
     5641 ggagatgatt aaatggcaag tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta
     5701 caaacaagtg gtggaagcag tgtctctttt gatttactgg ccggagcatt gggcgacgtt
     5761 ttaagttcta atgtttatgt agtaagtgca aaaggtaaag tactaggtct tcatttaaat
     5821 gatgttcaag acagttcagt tatagaagat gagtatacta agcaaaagaa attttcagat
     5881 gaatatactc aaaatgtgtt aaaaattgat gaaacattag aaaatttaaa tggtgagaag
     5941 atattagaaa tctttcctga agaacatgga agattacaaa aatatactac agtagttcca
     6001 atattaggaa gcggtcaaag attaggaaca ttggtacttt caagatattc aaattcattc
     6061 aatgatgatg atttagtaat agctgaatac agtgcaactg ttgttggtct tgaaatatta
     6121 agagcaatag gtgaagaatt ggaagaagaa atgagaaaga aagctgtagt tcaaatggca
     6181 ataggcactc tgtcctactc cgagcttgaa gcagttgaac atatttttgc tgaattggat
     6241 ggaaaagaag gtctacttgt agcaagtaag atagctgata gagttggtat aactaggtct
     6301 gtaatagtaa atgcacttag aaaatttgag agtgcaggtg tgatagaatc aagatcatta
     6361 ggtatgaaag gtactcatat aagaatactt aatgacaaac ttacagatga attaaaaaaa
     6421 ttaaaaaaca atcaataatc tatattttat aggtttagat tagaaaaggt atctatttca
     6481 aatagatacc ttttttagac gaattattaa ggtaatgata agtatattaa aaaggagtga
     6541 gaaaatgcct ttagcagata aaataagacc aaaaacaatg aatgatgtaa taggtcaaag
     6601 tcatataata ggtaatggaa aaatattgtc taagatatta caatctaatt ttttaccaaa
     6661 tatgatattt tttggtcctc ctggtgttgg taaaactaca gtagcggaaa taatagcaga
     6721 aagaagcaat aaaagcttct ataaaataaa tgctactaat tcatctttag aagatataaa
     6781 aaaggtgata tcagagcttg gaagtataaa taatgtaaat ggagtcctat tatatataga
     6841 tgaaatacaa agttttaata aaaaacagca acaatcaata ttagaattta tggaaaatgg
     6901 gagtataact ttgattgcta gcactacaga aaatccatat cattatgtat ataaagctct
     6961 tttaagtagg tctactgtat ttgaatttaa acctttagaa aaacttgatg tagaaaaagg
     7021 actgaaaaga gctgttgaag tattaaatga agatagctat atggatattg agtgcaataa
     7081 tgatgctatt gaagatatag caattctttc tgatggagat atgagaagag ccttaaatat
     7141 tttggaagtg gtagtctatt caactaaacc aaataaggat aatataactt atatagattc
     7201 agatgtaata agggcatcta catttaataa aattataaac tatgacaaaa atggtgatag
     7261 tcattatgat attttaagtg catttcaaaa atctataaga ggaagtgacc cacaggcatc
     7321 tatacactat ctagcaagac ttataaaagg tggggatttg ataagtatat gtaggagact
     7381 tttagtaata gctagtgaag atataggttt agcttatcca aatgctattg taattgtaaa
     7441 ggcatgtgta gactcagcta tgcaacttgg atttccagaa gcgaaaatac cattagcaga
     7501 agctactatt cttttggcta catctccaaa gtctaattct gcatgtatag caataatgaa
     7561 agctatggat gaactggata aagagtttgt taaggatata ccaaatagta taaaggatgc
     7621 tcactattct ggttctaaag aaataggtag gggttgtgga tacaaatatc ctcataattt
     7681 taaaaatcat tatgtaaagc aacagtattt accagatagc ataaaagata gtatttatta
     7741 cataccacaa gaaaataaaa ctgagaaaaa tataaaaaaa tatttagaat atctaaatag
     7801 tgatttttag actgaacttt atgtatcggg ggtagatatt atgattataa aacttaactc
     7861 aacttatcac agtagagtta tgaaatactt aaaaaaagaa cctgagtaca atttgtttat
     7921 aataggggat attgaaagat atggatatgg aaataatttt ttaaatatat gggcagatgt
     7981 gggtgaacat ggtgaaataa aggcaatatt acttaaatat tttgaattta tgatgtttta
     8041 ttctgatgga gagtatgatg ttgaaggttt ttataatcta cttcgtaata ctaattacga
     8101 agaaatatct gggaaaatat gtgctgtaga tgctttagca aagagattag gacttaataa
     8161 tttaaaggta gttgattttt gcaaacttca aactaaaaag tttttgatag ataataattg
     8221 taatgcaaag gttaaaagaa ttaggcttgg aaatctaaaa aagacagtga aactttatga
     8281 cttaatagat gaatttcaca gtacaacttt agagaattta aaaaatggac taaggacagg
     8341 tagaggatat tgtatagaga ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga
     8401 aaataggact catgcaatga taattggagt aggaacacat cctaaatata gagctaaagg
     8461 acttgctaca aaatgcctaa ttaaactatg ttcagagtta ctgagagaaa acaaaattcc
     8521 ttgtttgttt tatgataatg aagaggctgg aaagatatat aaaaaactag gatttgaaaa
     8581 cattggaaaa tggggaattt attcaaaata aaaaattaaa attaatcttg acaaaattag
     8641 tagggtataa taaaattatc ttgaaagtaa ttcctactaa aacagtatga attagatttc
     8701 tatatagagg tgataatata tgaagttgtc tactaaaggt agatatggac taaaagcaat
     8761 gtttgaatta gcattaaatc aagacaatgg accagtatca ttaaaattta ttgctaagaa
     8821 gcaaaaaata tcagatcagt acttagaaca aatattttca tctttaaaaa aatcgggtct
     8881 agtaaaaagt gtgagaggtg ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat
     8941 aacagtagga gatatacttg tagtattaga aggaccagtt gcattatctg actgtgtact
     9001 agatgaagat gtatgtgaaa actcaaacat gtgtgtaaca aaaatagttt gggaaaagat
     9061 gaaaaagggt atagaagatg ttatagattc aattactctt aaagatatga taaatgacta
     9121 taacaaaaat aaattagaaa atgatataac aaatattaaa aaatagattt ggagatgaat
     9181 gtaatggaaa aaagaagatt gtatatggat tattctgcaa caacacctat taaaaaagaa
     9241 gtattggatg caatgatgcc atacctaaca gattattttg gtaatgcatc tagttttcac
     9301 acattcggaa gagaagcaaa agatgctttg gataaagcaa gagaacaagt agcagccctt
     9361 ataaatgctg aacctagtga aatatatttt acagcaggtg gttcagaaag tgataactgg
     9421 acactagagg gtgtagctta tgctaataaa aacaaaggta atcatataat aacttcaaaa
     9481 atagagcacc atgcaatact tcatacatgt gaatacttag caaaacatca tggatttgaa
     9541 ataacttatt tagatgttga tagcgaagga aaagtagact taaaacaact agaagagtca
     9601 ataaaagata ctactatact tataagtata atgtttgcaa ataatgaaat aggaactata
     9661 cagcctatta aagaaatatc cgaaatagct aaaaaacata agatattatt ccacactgat
     9721 gcagtacaag ctacaggtaa tattccagta gatgttaaag aattaggaat agatttgatg
     9781 agtatgtctt cacataaaat atatggacca aaaggtgtag gagctttata cataagaaaa
     9841 ggtgtaagac ttcataactt tgtccatggt ggagcacaag aaaaaagcaa aagagctggt
     9901 acagaaaata tacctgcaat agttggatat ggaaaagcag cagaattagc taaggaaaat
     9961 atgcaaaatc atgtagaaac tttaactcgt cttagaaata agttaataga tggtgtgtta
    10021 gaaagaatac cttacacaag agtaaatggt agcttagaaa atagattgcc aggaaatgca
    10081 aactttgcat tccaatttat agaaggtgaa ggaatacttt tattattaga tatgttagga
    10141 attgctggtt caagtggttc tgcatgtact tctggttcat tagacccttc acatgtactt
    10201 cttgcaatag gtttaccaca tgaaatagca catggttcat taagacttac agttggagat
    10261 tttacaacag atgatgatat agattatata ctagagaact taccaaaagt aatagaaaga
    10321 cttagaagca tgtcaccact ttatgatgat gcgaagaaac aaggtttagt aaaatagaca
    10381 taaagataaa gcataaaaaa tagacataaa ttgagtagga ggaaaagata atgcaatata
    10441 gtgataaagt tatggaacat tttatgaatc caagaaatat gggagaaatt gataatgcaa
    10501 gtggtgtagg tgaagttgga aaccctacat gtggagatat aatgaagata tttttagaca
    10561 tagatggaga tgtaataaag gatgttaagt ttaaaacatt tggctgtggt tcagctatag
    10621 caagttcttc tatggcaact gaaatgataa aaggaaaaac tataaaagat gcattagaac
    10681 ttacaaataa agctgttgca gaagctttgg atggtttacc accagtaaag atgcattgtt
    10741 cagttttagc tgaacaagct gtaaaagcag ctcttataga ttatgctcaa aagaacaata
    10801 tccatatacc agaattggat ggatatgtta tagatgatgc tcatgaccat gatgttgaag
    10861 aagaagaata aaaataagtg gttgtgttat gagaagttta caatgattaa aaagaagtac
    10921 taatgatata gttaggacat aaaaaatagc tgtttttaaa atggctattt tttattgcct
    10981 aaattaagta ttttaacttg atttagaggt taattagtta acaaaataat ccataagatt
    11041 caaataataa agatgattat ttttttagaa aatagaacaa gctatatttt aaataagtac
    11101 aatacttgct ctatatggta taataataag taatgcaaaa gatgaggtga aatttgttta
    11161 tgaataaaaa agtaatgata ggtatgagtg gaggagttga tagctctgta gctgcatatc
    11221 ttttaaaaca acaaggatat gatgtcattg gtgttactat gaagttgtgg caagatgatg
    11281 atgttgttga aattgaaggt ggttgttgct ctttatctgc tgttgaagat gctagaaggg
    11341 tagcaaataa aataggtata ccattttatg ttttaaattt tagagaagtg tttaaagaaa
    11401 aagtaataga ttattttata gatgaatatt tggaaggaaa aactcctaat ccctgtatag
    11461 cttgtaataa acatataaaa tttgatgatt tttataagaa ggctagacaa ataggttgtg
    11521 attatgtagc tacaggtcac tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt
    11581 tgctaaaaaa atctgttaca gataaaaaag accaaactta tgcactttat aatcttacac
    11641 aagagcaact agagcatact ttattgccaa taggtgacta tgaaaaagat agagtaagag
    11701 aaattgctaa ggaaatgggt atggcagtac acaataagcc tgatagtcaa gaaatttgtt
    11761 ttgttaaaga caatgactat gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag
    11821 aaggtttttt tgtagatact aaaggcaata tacttggaaa acataagggt atattatatt
    11881 atactatagg ccaaagaaaa gggcttggaa ttacttttgg aaaaccaatg tttgtaatag
    11941 acataaatcc aataaataat accatagttc ttggagacaa tgaagattta tttaaaaagg
    12001 aacttattgc taaagatgtt aactttatat caatagatac gttagaagaa cccttaagag
    12061 ttcaagccaa aataagatat tcagcaaaac catcaccagc tactattcat agagtaggag
    12121 aagatactat taaaatagtt tttgatgaag ctcaaagagc tattactaaa ggtcaatctg
    12181 tagtaatgta tgatggagat attgtagtag gtggaggtat aatcgagaaa agtttataaa
    12241 aacatctaaa aaattaaaaa aataaacaat aaagtatatt aagagtaaat aaagtttata
    12301 aaataaattt attgtatatg aaaatatagt attgattaat tttgtaaaat ttagtaatat
    12361 aattagtata ttaattaaaa aacaaataca gtgataagga atagtaaaat atttgatgtc
    12421 ttacagagag agggaaatgc tggaagccct tagaatatga tatttgaagc agccttttag
    12481 tagcaatctt gaacaattta tcttttataa agaagattta agtagagatt gacggtagtt
    12541 acgttatagc taaggttttc cgaagatatc atactttgta tgataattag ggtggtaccg
    12601 cgattgtcct cgcccctata aatttattta tagggacgag gttttttatt taaacgaaaa
    12661 aataaaattt attaggagga acatgatatg gaaaagatgg gattaaatga aataagaagt
    12721 aagtttttag aattttttga gtcaaaaggt cattatgtag caaatagtta ttctttagta
    12781 ccaaacaatg ataagagttt gttacttata aactcaggta tggcaccttt gaaaaactac
    12841 ttttcaggtg tagaagtacc tccaagtgtg agaatgtgta catctcaaaa atgtataaga
    12901 acaggagata tagaaaatgt aggtataact gcaagacatg ctacattctt tgaaatgatg
    12961 ggtaacttct catttggaga ttactttaaa agagagtcaa tcaaatgggg atgggaattt
    13021 gttacagaat ggctaaatat accagaagat aagatatggg tgactgtata tgaagaagat
    13081 gatgattctt atgatatatg ggcaaaagaa atgaatttcc cagaagaaag aatggttaga
    13141 cttggaaaag atgataattt ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa
    13201 atatattttg atagaggaga agaatatgga tgtgacaatc cagactgtaa accaggctgt
    13261 gattgtgata ggtatttaga attttggaac catgtattta ctcaatttga tagagatgaa
    13321 gaaggaaatt atagtctatt agaaaacaag aatatagata caggtatggg tcttgaaaga
    13381 atgggatgta ttatgcaagg cgtagacact atctttgaag ttgatacaat taaatctata
    13441 ctagaagcag ttgagaagtt aacaggagta aaatatggag agaatccaaa aaatgatata
    13501 tcaataagaa taataactga ccacataaga gctgtaacat tcttagttag tgatggagtg
    13561 cttccatcaa atgaaggaag aggatatgtt cttagaagac tccttagacg tgctgctcgt
    13621 catggtaagt tattaggagt aaaagaatta tttttacaga aactaataga tgaagttata
    13681 aaagtaaatg ataaagcata tccagtttta gttgaaaaag aaagttatat taaaaaagta
    13741 gttggaatag aagaagaaaa attcaatgaa actatagacc aaggaacaga aatattgaac
    13801 tcttatatag aagtattaaa aaatgaagga aagactgtcc taagtggtca agaagctttt
    13861 aaactatatg atacttatgg cttccctata gacttaacta aagaaatatt agaagaagaa
    13921 catctatctg tagatgaaga agcttttaat gaagaaatgg aaaagcaaaa agaaagagct
    13981 agaaatgcta gaggaaatat ggatggagaa agttggaagg aagaccctct atcaaaatta
    14041 gaatctacag ttgacagtac atttaatggg tatagtgaaa tttatggtga aggaactatt
    14101 gaagctatag ttaaagatga tgagctagtt caaagtgcag aagaaggcga taaggtatct
    14161 atagtactag ataacactac tttctatcct gaaggtggag gacaagttgg agattgtgga
    14221 ttgataacta atgaaaactt agttttagaa gtattaaata ctaaaaaagg tgctaataat
    14281 agtataaaac atataggtat aataaaatca ggaagaataa gtaatgggga caaagtaaag
    14341 acactcgtag atagagaaac tagaatgtca gctgcaagaa atcacagtgc tacacattta
    14401 cttcataagg cattaagaga agtattagga gaacatgtta atcaagctgg ttctttagta
    14461 actccagaaa gacttagatt tgatattact cactttgaag caatatcaaa tgaagaatta
    14521 aaagtaatag aagaaaaagt aaataatgtt attttatcat ctttagacat aaaatgtgat
    14581 attatgaata taaaggaagc taaagaaaaa ggagctacag cactatttgg tgaaaaatat
    14641 ggtgatgagg taagagtagt ttctatggga gattattcta cagaactttg tggaggaact
    14701 cacttgacaa atacttctca agtaggaatg ttcaaaatat tatctgaagg tggagtagca
    14761 gcaggtgtaa gaagaataga agcaataaca ggaaaagctg tttatgaata cctaaaagaa
    14821 agagatggaa ttatctctga agtttgtgta aacttaaaat ctaaagaaga taatttaatt
    14881 caaagaataa gttctttatt agaagaaaat aaaaacttat ctaaagaatt gcatgatatg
    14941 aaagctaaga tgagtttaca atcagttgat tctatttttg attctaaagt tgaagtaaat
    15001 ggagttaatt tgataactaa taaatttgaa ggtatggata tggatacact aagagaaaca
    15061 gcagataatc taagagataa gcttggttct ggtgtagtag tacttgcaaa tgtagtagat
    15121 gataaagtta actttgtagt tactgccact aaagatgtat tagataaggg aatacattca
    15181 ggtaatattg ttagagaagt tgcaaaaata gctggaggaa aaggtggagg aagacctaat
    15241 atggcacaag caggagctag tgatgtatct aaggttgacc aagctttaag ctatgcaagt
    15301 gaggttatta aaacacaagt taaataacat tcactcaagg aggtttttag tttggaaaaa
    15361 gatttagact acactatgaa atttgaagga ataccagaag ataggatgag tgtaggagat
    15421 actattgatt ttgtatataa agcattagtt gaaaaaggat ataaccctat aaatcaaatt
    15481 atagggtatc tcctttctgg tgattctagc tatataacta gtcataaaaa tgcaagagct
    15541 ataattaaaa aatttgaaag agatgagata cttgaagaag taatcacgca ctatctgaat
    15601 agaaagtagg tcaaataaat gagaaaaaag attatgtcgt tgtttgcaat agtcattatg
    15661 atttttgtat caactgctag ctatggtttt gctgaagaaa aaggcaaggt catatttatt
    15721 gatatgaata ggaccaatct tggaagtatg atgaatattc caatcttgaa aaatgagata
    15781 gaaaagagag gctatgtagc tctcatgaat ataagagggg atcaaggtac tgatgataga
    15841 aggtcttatg caagtatggg tgcaggaaca agagctagtg taactactga agactatatc
    15901 aattttcaaa aagtagacca agatactgct aaagcttttg aagtcgcgac aggtcaaaaa
    15961 gctaaagaaa taaatgattt aggcattaat agatctataa atgaaaatct tgagtatgga
    16021 gaatatggag caaatttagg gtcattagga aaaactttat cagaaaatgg tttaaagaca
    16081 tctgtaattg gaaatgctga tataattgaa aataatcaat tagttaaaaa cagaaactta
    16141 tgtttagtag ctatggacca atatggaagg attgattcag gtaatattga taatattaat
    16201 aagaaagatt ctaaaatgcc atttggaata tctacagatt atgataaatt actagatgaa
    16261 actaaaaaat cctataaaaa tagtgatgct atatttgtag aacttggaga tacatataga
    16321 ttagatatgt atagattaaa tctaaatgat aatacttatg atacaatgaa gaaaaatatt
    16381 gtaaaaaata taaataaata tcttgaacaa gtattttcta tggttggaga aaatgacact
    16441 gtttatatag caagtgcatt tccaagtgac ctagattata agaataagag aagactttct
    16501 ccaataataa aatttagtgg agaagggaaa ggtctactgg aatctgccac aacaagaaag
    16561 gctggaattg ttgccaattt agatgttgga gtagatatac taggtacatt tggtcttgaa
    16621 aataaagaga tggtaggtag aagttatact ttagtaaata aagaggacaa tattagctat
    16681 ctaaaatcac tttatgaaag gatagtgtct atatcttcaa taaggtcaac tattataaat
    16741 ggctttgtag gcgtaatatc tgcttcttgg gtaattgcca tgtttgcgat tttctttaga
    16801 aattatatgc cacataaaga gcgtgtattc aaagtactta aggagtttat aaaattaggc
    16861 ataataatgc cactatcatt tatgttagct cctttgatga attttaaaac tccaatttca
    16921 atatctttgg gaatatttgc aactacgcta atattatact tgttaggtag aaaattattt
    16981 aaggacaatg atataaagca catgggattt tttgcaacac ttactatttt agtcatagtt
    17041 atagattctg tatttggaac atacttaatg caaaataaca taatgagtta tgatgctgtc
    17101 ataggagcta ggtactatgg tgtaggaaat gaatatgaag gtgtaactat agcaagtgca
    17161 gtatttggct tagcagtatt attacattat aagaaggtat ctaaattact tgttgtaatt
    17221 ttttcattaa taatacttat aactagtgca tatccttcaa tgggtgcaaa tgttggtgga
    17281 gctatatcag aatgtgtagc atatttgttg tttataatgc ttatatatga cattaaaata
    17341 gattttaaga aagctgtatt attagctgtt tcagctgtag ttgtcgtttt tgcatttgca
    17401 gctttagata tactttcagg tagtgaatct catttaggta tgtttgtaca gagaattttc
    17461 ctaaatggac caggagagat attacaaaca tttggaagaa aaattcaaat gaatatgcaa
    17521 ctagcacaga caagtgtatg ggtaaatatt ttacttgtag gaattggagt aattgcagtg
    17581 cttatattta aaccatctag acactttaga aagataatga ataattttcc aatcttgttt
    17641 aaagggttta ttgcatctat ggttggttgt ataattactc ttttagttaa tgattctgga
    17701 atagttgctg catctacagc atctatatat atacttatac cactaataat aataagtata
    17761 aatatgataa tatttgaaaa aaataactaa aaaaattttt actgttcata taggatttaa
    17821 ataaaaaatg caatatgatt attaatgtaa actggatgct tacataaaaa atcatattgc
    17881 aatttttatg attgattatg taaaattcta tgaagcagta agcctgtgac cttagttatg
    17941 agaggttcag aatatgtatt taatctatag aatataaata gataaaggag gaactatgtt
    18001 agatggaaga ataatgggtc ttgatgttgg tgataaaact attggagttg ctgtgagtga
    18061 tttgatggga ttaactgcac aaggtgtaaa aacaataaaa agagttggca agaaaaaaga
    18121 tattgaagag ctaaaagcaa taataaaaga aaaacaagta aataaaattg tatcaggatt
    18181 accaaaaaat atgaatggaa ctttaggtcc acaaggagaa aaagtaatca agttttgtga
    18241 attggtaaaa gcagaaacag gtattgatgt agagttttgg gatgagaggc tttctacagt
    18301 agcggcagag aggtctttat tagaagcaga tgttagtaga caaaaaagaa aaaaggttat
    18361 agatatgttg gcagcagtaa taattttgca aggatattta gattttaaaa taaattcata
    18421 aaagaactat aaaattgaat acttatgata tataatataa ataataaaaa taaaaataaa
    18481 tttgaggtga atgacattat ggaagaaaat ataataaatt taatagacga aaatggcgtt
    18541 gaaagccaat ttgaaataat attaacatta gaagcagaag gaaaagagta tgcaatatta
    18601 atgcctttag atgatgaaga agctgaagaa gctttaatct ttagaattga tgaggatgaa
    18661 gaaggtgaaa tcttaatacc tttggaaagt gatgaagagt atgaaactgt tgtagctgtg
    18721 tatactgcta taatggaaga agaaggttta aattacgacg aagatgaatc aaatggactt
    18781 aactagataa gtaattaagt ttgtatataa aaatcagata tttattatat ttgctttaat
    18841 taaaccttat taattgtaaa agcatttata gtatatatct gtttttttgt tttttattaa
    18901 aaataccaaa aaatttatga attttggtga ataatatatc ttttatgggt atatatttat
    18961 attatgagaa taaattaaat ttatttaaat gtcttgacaa aaaatgaatt atcaattaca
    19021 atattattta ataaagatta tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa
    19081 tggatttatt aaaagacaag ttaaaagaaa ctggttttaa gattacgcca caaagaagag
    19141 caatagttga aatattgctt aaacatgacc attcacatct aagtagtgag gaaatctatg
    19201 atttagttag ggttgactgt cctgaaatag gcttagctac tgtatataga acaatgcagc
    19261 tattggatga aataggatta atatcaaaac taaacttaga cgatggatgt ataagatatg
    19321 agattagtct acataaagaa gactgtcata atcatcatca tcttatttgt aaaaattgtg
    19381 gaaagataat ggaagctaaa gaagaccttt tagacaatat agaaaaagag atacaatcat
    19441 tgtacaaatt taaaatactt gatcatgatg ttaaatttta tggactatgt gacgagtgta
    19501 atggagtaag cgattcagaa gaataaactt ctattgctta atgtcgatgg aagaatagta
    19561 tagaactttt atattctatg ctattcttcc attttttatt ataaaccatc tataaaatgc
    19621 aaatatacag ttttcaagat gcttatagtt ttaatattga aaatataatt aatcctataa
    19681 gtacaaaaat gaatgatttt tctatgaaat tactaaatgg tcctccaaca gttaaaaaac
    19741 ctaaagaaat tttcttttcg cttagcggat aaaaaattgg tattccttgc tttgtaaata
    19801 aatctcctaa aaacatatga cttgcataac caatcgtacc ataaaaagat aaattattaa
    19861 tattagtgat taattctatc tgttttagta aaaaatatac tattatagtt gcaaatatgc
    19921 tatgagaaaa acttctgtgc tttaaccagg gaaaaattga caacattaga caaaaaattg
    19981 taaaatatat ttcaacttct atgatgtata agcatatagc taatagtata aatattaatg
    20041 atagaaaaac ttttcttaga aatgtgtggg ttagttttgc ttcaataatt ataattgcaa
    20101 caaatgtaat tattgcgctt aaataaaaat tatcatttat atttattgaa ataaagaaaa
    20161 taataatatt aacagcatat ataaaaattc tatatatgta tttggataca ttgtttttta
    20221 gaatgaaatt tgagaccata gaattagatt tatccaaatc gggtagtaca gcaaaagtag
    20281 ctgaaactaa aatatcaact aaagatatag gaattttaaa tattaaagat gtttggatag
    20341 ctgttaagat tcctattgca caatgagttt taccacgcat aaatcctcct aaattatccg
    20401 atattaaatc atataatatt tagaggatat caaaataaaa taggtttgtc aaaataatac
    20461 gttaaatgta ttttaaatat agcttaaaat atgatacgaa ttatggtaca ataagaataa
    20521 ctggtagcga gtgcgtgatt tgatattatt tatgattgta gaacgtgtta aaaaattaaa
    20581 tagacagaag gagatgatgc aattgttcaa aaagaacacc aacaagataa aggtaatggc
    20641 cttgggtgga cttaacgaag ttggaaaaaa tatgactgtt gttgagtata aagatgaaat
    20701 aattgttata gatgctggac ttagttttcc agaggatgaa atgttaggag tagatatagt
    20761 tataccagat ataacttatt tagtaaaaaa tagggataaa ataaagggta tatttattac
    20821 acatggacat gaagaccata taggagctct tccatatata ttaaagaaaa taaatgtacc
    20881 tgtatatggg gcaagactta gtataggtct tatacaagta aaacttaaag aacataaaat
    20941 gaataatgtt aagttaaatg ttataggtcc tagacaagtt ataaagcttg ataatatgga
    21001 agtagagttt ttgaaaaata accatagtat accagatgct tactctattg caatacacac
    21061 agaccaaggt ataatatacc acacaggaga ttttaagatt gacttgactc ctattgatgg
    21121 agatgttatg gatatgcata gaatatgtga gttgagcaaa aaaggagttc ttttaatgct
    21181 ggcagatagt actaatgcag aaaaaccagg atttactatg tctgaaaaga ctgttggggt
    21241 aggtcttgac gaattatttg caaaaggaaa tggcagaaga attatagttg ctacctttgc
    21301 atccaacata cacagattac aacaaataat aaatactgct gaaaagttta atagaaaagt
    21361 tgctatatca ggtcgctcaa tggtcaatgt agttggagtt gcaaaagaat taggatacct
    21421 agatatttct gatgacatgc ttatagattt aaatgatata tgtaagtatg aagatagtga
    21481 attagtgata ataacaactg gttcacaagg tgaacctatg tcagctcttg ctagaatggc
    21541 tttttcagag cacaaaaaag tagaaataaa gagtggcgat ttagttataa tatctgctca
    21601 tccaatacca ggaaatgaaa aattaatatc aagagtaatc aatttcttat ttgaaaaagg
    21661 agcagaagta gtatacagtg atatagctga tatacacgtt tctggacatg cctgtcaaga
    21721 agaattaaaa cttatacatg cattagttag accaaagttt tttatgccag ctcatggtga
    21781 atatagaatg ttaaaaagac atgctgaaat agctgaacag cttggtatgg ataaagaaaa
    21841 tatttttgtc atgcaaacag gagatgtttt agaattagat aaaaactctg ctaaggtagc
    21901 aaaccgtata caaacaggaa acatattagt tgatggtcta ggagtaggcg atgtaggtaa
    21961 tatagtttta agggatagaa agcatttatc agaagatggt cttatgatag ttgtagttac
    22021 tatttccaag gatgagggta aggtcttagc aggtccagat ataatttcca gaggattcgt
    22081 atatgtaaga gagtcagaag atttaatgga tggagcaaag gatataatta agaatgttct
    22141 aaatgaatgt gaagagaaaa atattaaaga atgggcttat ttaaagaata atattaaaga
    22201 aaacctaaaa gaatatttat atcaaaaaac taaacgaaat ccaatgatac ttcctatcat
    22261 aatggaagta taatataaaa aaactataaa tttgaaaatt agacctcttg taatttttaa
    22321 agtaatatca aaaatccaat tgatattatc ttaagaatta caagaggtct ttttaatact
    22381 catttaaaaa aaatatatat tacataaaat accaattata acaatctttt attagtaaaa
    22441 actacttata tataaatttt actgatagga ggattcttat ggatgatgtt tcaagacaaa
    22501 atgctataaa agctttaaaa caaacaaaaa tggaaatagc aggagagtat ggaatgaatt
    22561 atgaagatgc ttttgaaata atagaaaatg cgtctaataa aggtgtttta gaaggttact
    22621 ttaaaaagct tgaaaagaaa aaaaatttag gtcaaggtat atctaggcat ttagaataaa
    22681 aaaagttaaa tgtgagaaaa taagtttaaa taaactttaa gaggtgacat cgatgaaaaa
    22741 attagctagt actgctttgg ctatattaat tgcgcttact cctttaagct tttcttttgc
    22801 aaataataag gaaaatgctg atgccaacca actaaatata tcttcaaaat cagcaatttt
    22861 gatggatgtt ggtagtggac aaattttata tgaaaaggat gctcataaaa agcttcctcc
    22921 tgctagtgta actaaggtaa tgactatgct acttatagta gaagctttgg attcaggcaa
    22981 gattaagctt gatgatgaag ttcaagtaag tgaaactgca tctagtatgg gtggtagtca
    23041 aatattccta gagccaggag aaactcaaaa ggttgataca ttattaaaag gtatagctgt
    23101 tgcttctgca aatgatgctt gtgttgctat ggctgaacac ttagctggaa gtgttgaagg
    23161 atttgttgat agaatgaatg ctaaagcaaa agagttaaat atgaatgata ctcattttgc
    23221 aaatacaaat ggacttccag ttgcaaacca ctatacatct gctcatgata tagctttaat
    23281 gtctagagaa ctattaaaac acgaaatgat aagtaaatac ttaactacat ggatggataa
    23341 ggttgtagta gggaagaaac aagttacagt aggtcttgca aatactaata aacttataaa
    23401 gcattatcaa ggagcaactg gtgttaaaac tggttttact caagaagcaa aatactgttt
    23461 atcagcatct gcaaagagag gtaatactca tctagttgct gcaactttag gagcagagac
    23521 ttctccagaa agatttaatg atgcctctag ccttttaaac tatggatttg ctaattatga
    23581 aagtgttaag ttatgtagta aaggtgataa tatagcaaca cttacattgg ataaggctga
    23641 tgaaaacaaa gttaaattgg ttgctaaaga agatttaaat gctcttatta aaaaaggaag
    23701 ttctaaagaa tttgagaaga aaattgaaat agtaaaaaat cctaagatgc caattaaaaa
    23761 gggaactgtt ttaggaaaaa taaagatatg taaagataaa aaagtaatag gcgaagtgga
    23821 attgataaat actaaggata taaataaggc aagctattta caaatgcttc aaagaataat
    23881 tgataatatg atataaataa ttatcagatg aaatataatt ataatacaca aagatatgat
    23941 tataatacac ataaaaaata cccttatgta attatttgcc ataggggtat ttttatttac
    24001 catatagaag tattttataa tcttatgatt tttatagaaa tatagaaaag tttatgtatc
    24061 ttaaaatatc atttgattta atatcatata tagggttgtt taacaattat ctaagtaaag
    24121 gcatactacc agtaagttta ttcactttct ttttattccc ataaattgct atcccaaaat
    24181 actgtaactc atcttctgga acactagcaa tcttttgtgt aaattcttca taaagattgc
    24241 aactttgtgc tacatctgaa aaatctacaa cagttaaatc gttaaattcc aaagtatata
    24301 attttttacg taaatctttt attatttctt tgtttccttt taaaataggt acaggaatgt
    24361 caataattcc taagtggcta ttacctgttt tgtcaattac atctgggcca acagtttctg
    24421 gggcatgttt tcctagtgta attcccatta tagctgctgt attggatata attcccattg
    24481 gtaaattttc gtctattacc atgacacatt ttgaattttt agtttccatt tttatttctc
    24541 ctttttacct tatttaaagt tgtttgaatc atcatttata aatatatttt gatactgctt
    24601 tggtgtaaga ccaataaaat tcttaaaaaa gtttgtaaag tgactttggt ctacaaatcc
    24661 tgtttgtatc gcagcatcta taggttctac gcctttttct aaaagctttt tagctttgtt
    24721 tacccgtata ttttctaggt atctatatgg tgtaatacct tttaatttcg taaaattacg
    24781 aagtagagag tacttattca tacctgctat agtgcttaat tcgtcaagta ctatatgttc
    24841 agcataatta ttttctagat aatcacatac tgcttgaatt tctatatttg tattttccaa
    24901 gttagattgc aaatttggct ctgtatgttc ttcaattaat tgttcaatta ggaagaaaaa
    24961 aagctcttcc tttttaaaat ccaattcttc ttccatgatt atatagtgta attcttgtag
    25021 tagaggtact agctcactgc gaaatataac aggttgatta aattttggta aataattttt
    25081 acctgtaatt tcaaagacgg tttttttcat tatttctggc ttaatattaa tacatctgta
    25141 atcaagtact ttatcatcta tttgttcaca agtatgacta tcaaaagggt taaaaagtag
    25201 caaatctcca gtacttgtgg tatactcttt atttttacaa gataaatatc tttgaccttt
    25261 ttctataaaa ccaattacat aatgttcatg aaaatggttt gggaattttt gcattatgcc
    25321 tttaaatcta tatgcttcaa tttttaattc cctatcaaag catactgtgc gttcttcttg
    25381 tctcacttag gtaacctcct ttcatgttat acaaagtgta acacatattt ttttatactt
    25441 cttgtataat attgactaag agacaataat gtcaattcat gtcaatataa tattacactt
    25501 acagtaggtg taattattat attttcacaa tgaatatgat ataataataa aagttataaa
    25561 ataaaaagca aattttaatt ttatgcctag aagttaaaat tattataatg taaaataatt
    25621 ttgattataa gctatttaat tgttataata atattaaaaa tttaatttag gagatgtaca
    25681 ttaatgttaa gtatgaatat aggagaaata gtagcaagtt tagttggtat agctatggca
    25741 atatcaatac atgagtttgg ccatgcttat tcagctcact tattgggaga tgatacagct
    25801 aaagcttatg gtagaatgac attaaatcct gtaagacatg ttgacccaat aggtcttttg
    25861 gctatgttta tccttaagat agggtgggca aagccagtac cagtaaatcc aaataacttt
    25921 aaaaactata agataggaaa cctgatagtt tcacttgcag gcgttttttg taatgttttg
    25981 actgctatac tgtgtgtaat tatcaataag tatgtgcata tgtacgctat aaatacaata
    26041 gctggatatg tgcttttata tagtatggga tttgcagctt ttaacttact tccgatacct
    26101 ccacttgatg gatggggagt tatatctaca tttgtaccat ataaatggaa tgaatatttg
    26161 tataaatttg aaagtatgag ttatataatt ttattaattg ctttatttac tggtatatat
    26221 caaataatac tatcaccaat atatacagtg ttgctaaaaa tagtatattt atttgtttaa
    26281 tataataaac agattaaaga gggattgata tgaagtataa tatacaatta caagtctatg
    26341 agggaccatt agatttactt tatgatttaa taacaaagca taaaatagac ataaaggata
    26401 tatctataat agatataact aagcagtatc taaattattt gaaaatgcta gataaaatgg
    26461 atttagagat aactagtgaa tttataacta tggcatcaaa acttttagag ataaagtcta
    26521 aatatctgct ttataagcaa aaagatgaag aagaagaccc aagaatagaa cttatggaaa
    26581 agttagaaga atatagaaag tttaaggtag catcacaaga tataaaagaa aatataactt
    26641 atgttaatga gagattttat agaaataaag aagagataat tattgatgac aatgtggatt
    26701 tagaagatat atcaattgaa gcaataaaaa atatacttcc atacatattt aaagttaaaa
    26761 catcacagat agaaaatgct aatgatgaga aactagataa aatagtaagg aagaaaataa
    26821 tatctgtaga agaaaaaata ttatatataa gagatattat aaaagataag attgaagtta
    26881 cttttacaaa tattataaaa tcatatgaaa atgatgaaat aatagctact tttttatcta
    26941 tattagagct tataaaagaa aaagaaatag tcgtagtaca agatatattt tttgatgata
    27001 ttctaataag aaaaagttcg gagtgttaaa atgaaaagag aagatataaa gtatattata
    27061 gaatctgtta tgtttgcata tggtgaacca ataagtataa aagaattaaa ctatataata
    27121 aataaagaac tttcatcaaa agaaatagag attatgttaa atttacttat tgaggagtac
    27181 agagaacaaa atagaggtat tcaaataatc aagttagaga ataagtacca aatgtgtaca
    27241 aataaagact atgcagaata tataaagaag attattgaac caaagaaaaa gaaaagtttg
    27301 agccaagcta cattagaaac tcttactata attgcctaca aacaaccaat aacaaaggta
    27361 gaaatagagg atataagagg agtaaaatgt gataaggtat tacaaacttt gtttgaaaat
    27421 gaacttataa gagaagctgg tagattgaat aagataggga aaccaataat ttataaaaca
    27481 acagatgaat ttttaaagtt gttgaatata gaaagtcttg aagaattgcc acctatagaa
    27541 aattatcaag aagtggtaac aagtgaataa aaatataaaa tcacaataat ttagtatgta
    27601 attttaaatt ttaaaagatt taaaatgctc taagttgttg tgtttttttg tataaatata
    27661 aaattataat tctcttaaaa taaatatgga taattaagta taaatagtaa ttttttgatg
    27721 ataatataaa catggagctt aatgaattat ttaattttaa gtatttaata atagctgtaa
    27781 tttttattat aagtgtaact atgttgattt taatatctca tataaacatc ttaataatag
    27841 ctgatataaa aaacaaagat atttgtttaa agcttaatat aaaatacatg tttaatttaa
    27901 taaatataaa tagacaactt tatccagctg aaaatagtaa aaataatgat aaaaaagagg
    27961 ggatgaagaa taatatagat tcaagtatat tattagctga tgaccttttg agcatttata
    28021 gattattaaa aaagataaaa atacatgaac tttattcaaa tattaatttt ggaactggaa
    28081 atattggctt aacatcttct gtatatgtat tgataaatac tttgtatgga aatttattta
    28141 atatgattga tgcagaaaaa atgtatttaa atgtaaatcc agattttaca aaagattatg
    28201 ttttaggaaa tataaggata catataaggc caagaataaa agctttattt aatattataa
    28261 taatgattaa taaaattatg aataaaaata aaggaaataa ggaaggtgat agtaatgaga
    28321 gcaacaggtt cgatacagag tcttatggaa acaactcttg aaacaattaa aggttctata
    28381 gatgcaaata ctattatagg agaccctata aaaacagata ctacagtggt tgtacctata
    28441 tcaaaggtta caattggctt tggtatagga ggtggagaat actcaaaagg atatgatgat
    28501 aaagataggg aaattgaatt aaaaaatgaa aaatcagata cgaattttgc tggaggtagc
    28561 gcaggggcta tatctgtaca accagtagca tttgtagtag ttgaaagtgg agagactaga
    28621 ataatgagtt tagatagtaa tatcaatcta gtggataata tattatctat aactcctaga
    28681 gtattggaga aaattcaaaa tatatcacaa agtaataaga atcaagataa aaacaatatg
    28741 taagaataaa atatttttaa atatgtatat agttttaagt aaagtagtgg ggttaatata
    28801 aatcccacta ttttgttgtg tactttaaac atttatatct aataagattt tagtttaaaa
    28861 caatttaaat agataataaa gttgatatat agtataatta taaaaagaaa agaagtatta
    28921 tattatagat atataaacgc atttgagata taagagggag ttataaatca ttagttttga
    28981 ggtgaatgtt atgagcgata aagaattatc tattttgttg ttagaaagta tgaataattt
    29041 tcataattta ataaagatta ttaataacga aagatataaa aaagataagg ttcttacaga
    29101 aagacagttc tttgccttag taaaaataag aaaacatgat aaaatagagc ttaaaaatct
    29161 aagtagagat ttgcatgtat ctacttctag tctgtgtata ctacttaata aattggttga
    29221 gcaagagtat gtatataggg aagaagatag tagagataga cgaaatactt tttatggaat
    29281 aactaaaaat ggagaaaaga tattggataa tgaaatacta aagtttgtat ctataataag
    29341 tgataaaatg gattgcctag atatagataa taaagataaa ttatttactt ctttggaaga
    29401 gtctaaaaat ataatagaac aattatttta aaaaggagaa tttattatga atataaagta
    29461 ttctaaagag atattaaaaa gtttagaaaa tgctataaca gtagtagctc tatttgaggg
    29521 agaagatgtt tctacgacat ttgataaaat aaatatgata agcagaaatt ttaattctaa
    29581 agaaaatagt atagatactg taacattaat tgaaaataac aatttggtaa atgtttgtct
    29641 tgttggtttt ggtaagagag agttattgaa tagagaaaaa attagaatca taggtgggaa
    29701 tttgtgtaaa aaattaaaaa atatattgaa aaatgaagca tataagagta gtgatgttaa
    29761 tgtactgaat cttaatttaa atagtgatga aataggttgt tttgtagaag ggatattact
    29821 tggaaattat aagtttgata agtataagac aaaaagtaag gattttgaaa gaaaagaagt
    29881 tgatactata actatattta ctgaaaaaga agttgagtgt caaatagaaa aggctaaaat
    29941 attagctaat tcaactataa tagctagaaa tctagttaat gaaccatcaa acgtaatata
    30001 tccaaaaaca ttagcagtag aaacagttaa acttggtgca gagtttgggt ttaatgttga
    30061 tgtttatgaa gaagaaaaaa taaaggcatt gggaatggat gcttttttcg ctgtttcaag
    30121 aggttcaatt aataaaccaa gattcatagt aatgagatac tttggagata aagaaagtac
    30181 agatatatta ggtctagtag gtaaaggatt gacttatgat actggtggat attctttaaa
    30241 atcaaatgct tctatgctag atatgaagac agatatggct ggtgctgcga gtgttatagg
    30301 tgctatgtgt gctatatctc aaagtaaatt aaagaaaaat gttatagcag tagtggcagc
    30361 atgtgagaat gctttatcag gaggttcata caaacctgga gatataatta gttctatggc
    30421 aaagaaaact attgaagttt taaatacgga tgcagaaggt agacttacat tagctgatgc
    30481 aatatactac attataaaca atgaaaaagt aacaaaggtg gttgatgtag ctacattaac
    30541 tggtgctgct ttaatattgt taggtaatgt cgcaactcct atagtaacaa ataatgatga
    30601 tttttattgt gaattagaaa aagctgccac tttatcaggg gaaagggtat ggaagatgcc
    30661 aatttatgat gaatttaaag atatgataaa aggagaagaa gcagacctta aaaacactgg
    30721 tggtaaaaat gctggatgta taacagcagg agcttttatt ggtgaatttg ttgggaatac
    30781 tccttggata catatggata tagcggggac aagtacatca tcaaaatcaa ttggatacaa
    30841 agcaaagggg gctacaggag aacctgttag aacattgtac tatttggcag aaatctaaaa
    30901 aacaattata aaatataatt tataaagcca cgtggatatt tttgtaattt aagattatct
    30961 atgtggcttt attttttatt taaataaatt atttactctt tttctttttt tcaagtataa
    31021 aagtatctct attaaataaa agagcaggta tgtacataat actagtaaca aaaataaacg
    31081 taaaaatagc agttgtgcta tgacttaaaa aaccataata gtacctagag ttggtaaatg
    31141 tgtttagtaa aaatatgaat gaaaaataaa taaaacatat agctaagtta tctctaagtg
    31201 catatctgtt aactctaaat tttctaacat caaccatata tatatctgca taaattatca
    31261 agcacttaga gaagataaag ataaaatata ttaagctagg aaattcatag cccaagtttt
    31321 tagaaaaaat gagttctcca actgaacaaa ctaaactcca agaaaaaaat atattaaaaa
    31381 gatgatactg cttaaaaatt agtatggcta tacacagata tatacaaata taattgattc
    31441 caattggtaa agagtttaga gtactataat tacctgaaga aataagtaat aattgctcta
    31501 gagccataat tattacaagt aatgtacaaa ttatcttctc aactgtatag ctataaggta
    31561 aaatattttt ggttagtttt ggacaatagt ataaaaataa tccaaatata aagagactta
    31621 caaattgttc actagaaaaa agaaaaattt taaacatata acattctctc ctataataaa
    31681 tttaatatgt atctatataa atatattcat tatatctcaa tttaatatat aaaaaagata
    31741 ataatagtta gattttttat at
//
LOCUS       DAFKMX010000022        30701 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFKMX010000022 DAFKMX010000000
VERSION     DAFKMX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.022"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(142..711)
                     /gene="yfcE"
                     /locus_tag="LZB08_000884"
     CDS             complement(142..711)
                     /gene="yfcE"
                     /locus_tag="LZB08_000884"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBM7314399.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(736..1536)
                     /locus_tag="LZB08_000885"
     CDS             complement(736..1536)
                     /locus_tag="LZB08_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBM7314400.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1671..2531
                     /locus_tag="LZB08_000886"
     CDS             1671..2531
                     /locus_tag="LZB08_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314401.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            2531..3217
                     /locus_tag="LZB08_000887"
     CDS             2531..3217
                     /locus_tag="LZB08_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBM7314402.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3269..3811
                     /locus_tag="LZB08_000888"
     CDS             3269..3811
                     /locus_tag="LZB08_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314403.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3890..4357
                     /locus_tag="LZB08_000889"
     CDS             3890..4357
                     /locus_tag="LZB08_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBM7314404.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            5066..6202
                     /locus_tag="LZB08_000890"
     CDS             5066..6202
                     /locus_tag="LZB08_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBM7314405.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6261..7043
                     /locus_tag="LZB08_000891"
     CDS             6261..7043
                     /locus_tag="LZB08_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBM7314406.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7062..8072
                     /locus_tag="LZB08_000892"
     CDS             7062..8072
                     /locus_tag="LZB08_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBM7314407.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8091..8888
                     /locus_tag="LZB08_000893"
     CDS             8091..8888
                     /locus_tag="LZB08_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBM7314408.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            8986..9831
                     /locus_tag="LZB08_000894"
     CDS             8986..9831
                     /locus_tag="LZB08_000894"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBM7314409.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            9918..11093
                     /locus_tag="LZB08_000895"
     CDS             9918..11093
                     /locus_tag="LZB08_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBM7314410.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11289..12191
                     /locus_tag="LZB08_000896"
     CDS             11289..12191
                     /locus_tag="LZB08_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBM7314411.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIYEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12205..13209
                     /locus_tag="LZB08_000897"
     CDS             12205..13209
                     /locus_tag="LZB08_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBM7314412.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKNDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNITFYEEK
                     "
     gene            complement(13353..13580)
                     /gene="acpP"
                     /locus_tag="LZB08_000898"
     CDS             complement(13353..13580)
                     /gene="acpP"
                     /locus_tag="LZB08_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBM7314413.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13752..14177
                     /locus_tag="LZB08_000899"
     CDS             13752..14177
                     /locus_tag="LZB08_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314414.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14225..14431
                     /locus_tag="LZB08_000900"
     CDS             14225..14431
                     /locus_tag="LZB08_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314415.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14613..14792
                     /locus_tag="LZB08_000901"
     CDS             14613..14792
                     /locus_tag="LZB08_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314416.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14826..15026
                     /locus_tag="LZB08_000902"
     CDS             14826..15026
                     /locus_tag="LZB08_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314417.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15279..16295
                     /gene="ccpA"
                     /locus_tag="LZB08_000903"
     CDS             15279..16295
                     /gene="ccpA"
                     /locus_tag="LZB08_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBM7314418.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16418..16816)
                     /locus_tag="LZB08_000904"
     CDS             complement(16418..16816)
                     /locus_tag="LZB08_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314419.1"
                     /translation="MKKLDAESLEKMAKQKNIDKDKIEKMADSYKGKSESELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDTIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            17028..17939
                     /locus_tag="LZB08_000905"
     CDS             17028..17939
                     /locus_tag="LZB08_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314420.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESIL
                     DLVRGIKKLNNIDIVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDDN
                     EELDYEDEELDCEDKELDYDYDELTDEGEIEANEDELVEDKNTGYENIENNEDIVYDE
                     ELYNINEQESEHNYNEKNTKTRAKKKR"
     gene            18054..18234
                     /gene="ssrS"
                     /locus_tag="LZB08_000906"
     ncRNA           18054..18234
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LZB08_000906"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18417..19634
                     /gene="cdeC"
                     /locus_tag="LZB08_000907"
     CDS             18417..19634
                     /gene="cdeC"
                     /locus_tag="LZB08_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBM7314421.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19735..21057)
                     /locus_tag="LZB08_000908"
     CDS             complement(19735..21057)
                     /locus_tag="LZB08_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBM7314422.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21108..21902)
                     /locus_tag="LZB08_000909"
     CDS             complement(21108..21902)
                     /locus_tag="LZB08_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBM7314423.1"
                     /translation="MFYDYHMHSSFSTDGKSTLEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22012..23457
                     /locus_tag="LZB08_000910"
     CDS             22012..23457
                     /locus_tag="LZB08_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7314424.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23486..23695
                     /locus_tag="LZB08_000911"
     CDS             23486..23695
                     /locus_tag="LZB08_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBM7314425.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23715..25109
                     /locus_tag="LZB08_000912"
     CDS             23715..25109
                     /locus_tag="LZB08_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBM7314426.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25349..26101
                     /locus_tag="LZB08_000913"
     CDS             25349..26101
                     /locus_tag="LZB08_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7314427.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            26127..26540
                     /locus_tag="LZB08_000914"
     CDS             26127..26540
                     /locus_tag="LZB08_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7314428.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            26544..27287
                     /locus_tag="LZB08_000915"
     CDS             26544..27287
                     /locus_tag="LZB08_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBM7314429.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            27287..27757
                     /locus_tag="LZB08_000916"
     CDS             27287..27757
                     /locus_tag="LZB08_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314430.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            27774..28532
                     /locus_tag="LZB08_000917"
     CDS             27774..28532
                     /locus_tag="LZB08_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7314431.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            28516..29358
                     /locus_tag="LZB08_000918"
     CDS             28516..29358
                     /locus_tag="LZB08_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBM7314432.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(29518..30423)
                     /locus_tag="LZB08_000919"
     CDS             complement(29518..30423)
                     /locus_tag="LZB08_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7314433.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        11957 a         3415 c         5446 g         9883 t
ORIGIN      
        1 aacaagtaaa ccagattcta tttaattaga agtttaaggt attgtactta aacttctaat
       61 tgagatagaa ataaaatggt ttacttgttt ttattatgag tttaataaat taaagcttat
      121 tttattttta ttaaaaattt actaatcatt gatatttatt atatttccag tattgatgtc
      181 aattaagttt atatcaagtt caagctcatc ctcatcatca gtttttatga tatctataat
      241 tgcgacagaa tggcttccat cttttgggat agaagtactt cctggattta ttactattaa
      301 attatcatcc atgtatagtt tttttacatg agtatgtcct aaaactaata tgtcaccacc
      361 cattttcttt gctttgtcaa taatttcttc ttctgattct atataaccgt gattcaatat
      421 aattctaatt tcaccaaatt ggctcatgac ataaggactc tgaataggat gttctataac
      481 catctggtca acatctgcat cacagttccc ttttacaatc acaatgtttt ctaatttatt
      541 taatgtctca ataaattttt taggattata tccttctgga atatcatttc taggtccatg
      601 atataagaca tctccaccat gtaataaaac atcacaatca gataatacat ttaacgcttt
      661 ttcaaagtaa agtaagctac catgagtatc actcattatt cctattttca tattaaaaat
      721 cctcctaagt acagtctata aaaatttttc agatactcta tgccaaaact catagtttga
      781 cattctaagt aattttattt ttgcatcaga tatactaact tttatatttt cgatttgtct
      841 aaatctgtgc tcaactccat caacgacaat caatacagaa tcttcaaatc tatattctgg
      901 tgcaatcttt ataacagatt catttgaaca tattatactt gatgtaaagc acctatatgc
      961 attggtattt atcggatgta aaggtgtaat ctgcataagt tcaagattta tatcaacaat
     1021 actacctcca gcagaatagt tataggctgt tgaacctgtg gaagtagaaa taatcatacc
     1081 atctccacta aaattttgta tatgtttatt atcaaggctt aaatttaggt gtatagtcct
     1141 agacttgtct cctcttataa caacctcatt tacagcaagc atattagaac cactagtagt
     1201 tgtatatact tccgcattaa gaagtgacat ttcttgaata atataatctt tttttgtata
     1261 agcttcaata aagctatcaa tcttatctgg taaaatatca ggaaaaaacc ccagatgacc
     1321 tgtatttatt cccattatgg gaatctcagg aaaatcgaaa tctctaactg ttcttaaaaa
     1381 agagccatca ccaccaattg atataataag ctctgtgtct ggataaatat ctgaatacac
     1441 ttcaaagcca gcattgataa gttttctagt taagagttct ttagtctcaa gggacttatt
     1501 taattggttt gtatttatag ttataattct tttcatatat tcacctcttc atactttgta
     1561 ttataacata gttttataat tcttaattta taaatttata aatgaccttt gtaatgaatg
     1621 tgttaaaata tgtataggtg ggtataaaaa aatgcataag aggtgaagat gtgtttagca
     1681 taaggaatat cacaaaaaaa ttaggaaaat tcaagttaaa taatataaac ttagagttaa
     1741 aggaagggga tattgttggg attataggac caaatggttc gggaaaaacg actttaataa
     1801 aaattataat ggggattatt gatgcagatg aaggtgaaat tgaactttgt aatgaaacta
     1861 ttgaaaattc tccaataagc tttaaaaaca atattggttt tgtatatgat tctttacaat
     1921 tttatccgca tttaaaggtt aaggaattta gaaaaatagt ttctttattt tataagaatt
     1981 ttgatagaga aaggtttgat gaatacctta ataagtttga tatagaagaa aatatgcata
     2041 ttgaaaattt atctaaaggt caatctgaaa agcttatgtt atcaagtgca cttagtcaca
     2101 atgctaagtt attgattttg gatgaaccaa ctgctgggat agacccaatt gtaagaactg
     2161 aaataatgca gtacttacaa gattttgtga aaaatggaag tagtagtgta ataatttcta
     2221 ctcataatac tgataattta attaaaattg ctgattatct tgtttttata aatagaggaa
     2281 atcagatttt tactgttaag aaagaattaa tagaacaaga atataagata attagggcaa
     2341 ataaagcaga gttagaagca attaaagaaa gcatagttgg tgtgaaagag tataaatatt
     2401 ataatgaggc acttgttaaa gttggagata gcttgaaagt aaaaagccta ttgatagaaa
     2461 ttgataagca taaggtcaaa aatcctacta tagaagaact aatgtattat tatgtaaatg
     2521 aggtgagatg atgttcaatt taataagaaa agatttaata atagggatta gttcagatgg
     2581 tatccgtaat ttaaagtaca tattgctatt ttttgtgttt tattttttcc ttaattcaat
     2641 ttcatattat acagtaagta ttgttgttag ttatttgata tttataaata cgtttgagtg
     2701 tgattatgaa aatgattcaa ggatttttat aaggagtatg ccagttagta tagaagatat
     2761 agtttactca aaatatttat taggtgtagg attaataatt tctgtaacaa taattgtgtc
     2821 cttactaagt aaattgacat cactagtgtt ctttagaaat atggttttaa atgatgtatt
     2881 tttttctgta aatatatttt tagcaatttt gacgatttta ctgcctttaa tttttaagtt
     2941 tggatatgga aaaatgaaag tttgtggttt tatagtaagt attttaattt actttgtata
     3001 tggaagtctt ttgaagatga ttagtatgat tgtatatcaa gtaaagcatt ttaattatag
     3061 taaagttgga ggagtatatc tttcaaatta tgtaactgat atagctaata ctaagtatat
     3121 aaatttatat agtatgacat ttttaacaat aataattttt ataatatcaa tgtatttttc
     3181 tattaagatt tcaaaaaaga ataagtttaa ttattaaaat tttaattaat attatagcac
     3241 aatagcacaa aatataagga gactaaaaat gaagaagttt ataaaagttt cattttttat
     3301 tttggggatt tttacactat tcatagctat aaataaatta gtatatagag atatggtaag
     3361 tgtcaaagat tatgaaataa atcatttaga ggaaattgtt tttgattttg atagcaggag
     3421 aagtagagat gttggtacta ttgtaagatt taaaataagc aataatagta aatatagtta
     3481 taaattaaac agtgctaaaa tgaagtttga aaattgtgtg gaaaataaaa agggaatgaa
     3541 taagtgttct gcatatgtaa atgtagattt atttaatgaa aaaaattcta taactgaaag
     3601 aactctgaaa tatggaataa aaccaaataa tcaaggttat gttgattttg taattccaaa
     3661 aggactaaat tttgattata ggtactttaa tgaatctggt atgagtgtag agtataaagg
     3721 agaatatatt gtcaatattc caatggcaaa aggactttat ctagtagttg gaaaaaataa
     3781 tgatacatgg aatgctaata ttatggaata atttgaaaaa taaatcaatt atagatataa
     3841 ttaagaataa tatgaaattt ataaaagttc agactaggag gggtatttaa tgtcaataag
     3901 taatgtaaga gagtatttta aacaatttgg caaggaagac agtatattag aatttgaaca
     3961 atctagtgct actgtggaac ttgcagctga agcagctggg gttattccag caagaatagc
     4021 taagacactc tcatttaaaa taggagatga tgcaatttta attgtgacag ctggtgatgc
     4081 taagattgat aataagaagt ataaggctga atttaattgt aaggctaaaa tgttgacacc
     4141 agaagaagtc ttggagttta ctggacatgc tattggtgga gtctgtccat ttggtttaaa
     4201 aaattctata aaagtctatt tagatgattc aatgaaaaga tttgatacag tatttcctgc
     4261 atgtggcagt agtaactctg caatagaact tacatgtgaa gaaatggaga agttttctaa
     4321 gtgtgaaaaa tgggtagatg tttgtaagaa ttggtaaaaa aatatagtaa actatagtag
     4381 gaattagtaa aaaactacct ctgtatacaa tctatacaga ggtagttttt ttgtgtaaat
     4441 atttataaat ctaagaggaa aatctaattt aaactaataa tccaaaataa agttaaaaaa
     4501 ttctgatttt ttttcgaaac aataaaaaaa tgacaaaaaa gtggaaaaaa tgtaataaat
     4561 attgttaaaa aaataattag aaattattga aaaattatcc tttgagtgat actattatag
     4621 atgtaagata tttaaaaata aaaaatagtc agataaaaat acgattattt agctctatta
     4681 aaataatttt tatacgataa aaatcttctg tacatggcta aaaaacagat gtacagacaa
     4741 tatttaatgt ttaatatacc ctaatataaa tatcgtaaac aaattatttt atctgtaacc
     4801 agtgtgaaag aaatcttatt ctaacttgaa atttcacaaa attccaaaaa gcgagatttt
     4861 ttttaagtat agttgatttt aaattaacaa tttatgatta aattaatgtc ataattggtt
     4921 agtataatca catataaata tagagcgtag tttgattaag gttataaatt tctcgacgta
     4981 tattcaaata ccttattata gatgttgccg agattttaat taataaatga aaataaaata
     5041 tttataaaat actgaggagg ctattatgga tttaaattct aaaaaatatc agatgcttaa
     5101 agagctatat gtaagcttcg ctgaaaatga agttaaacct ttagcaacag aacttgatga
     5161 agaagaaaga tttccttatg aaacagtgga aaaaatggca aaagcaggaa tgatgggtat
     5221 accatatcca aaagaatatg gtggagaagg tggagacact gtaggatata taatggcagt
     5281 tgaagaattg tctagagttt gtggtactac aggagttata ttatcagctc atacatctct
     5341 tggctcatgg cctatatatc aatatggtaa tgaagaacaa aaacaaaaat tcttaagacc
     5401 actagcaagt ggagaaaaat taggagcatt tggtcttact gagcctaatg ctggtacaga
     5461 tgcgtctggc caacaaacaa ctgctgtttt agacggggat gaatacatac ttaatggctc
     5521 aaaaatattt ataacaaacg caatagctgg tgacatatat gtagtaatgg caatgactga
     5581 taaatctaag gggaacaaag gaatatcagc atttatagtt gaaaaaggaa ctcctgggtt
     5641 tagctttgga gttaaagaaa agaaaatggg tataagaggt tcagctacga gtgaattaat
     5701 atttgaggat tgcagaatac ctaaagaaaa tttacttgga aaagaaggtc aaggatttaa
     5761 gatagcaatg tctactcttg atggtggtag aattggtata gctgcacaag ctttaggttt
     5821 agcacaaggt gctcttgatg aaactgttaa atatgtaaaa gaaagagtac aatttggtag
     5881 accattatca aaattccaaa atacacaatt ccaattagct gatatggaag ttaaggtaca
     5941 agcggctaga caccttgtat atcaagcagc tataaataaa gacttaggaa aaccttatgg
     6001 agtagaagca gcaatggcaa aattatttgc agctgaaaca gctatggaag ttactacaaa
     6061 agctgtacaa cttcatggag gatatggata cactcgtgac tatccagtag aaagaatgat
     6121 gagagatgct aagataactg aaatatatga aggaactagt gaagttcaaa gaatggttat
     6181 ttcaggaaaa ttattaaaat agataaatat taaaatatac acaataggtc gatattaaaa
     6241 tagatatgga ggaaggattt atgaatatag tcgtttgtat aaaacaagtt ccagatacaa
     6301 cagaagttaa actagatcct aatacaggta ctttaattag agatggagta ccaagtataa
     6361 taaaccctga tgataaagca ggtttagaag aagctataaa attaaaagaa gaaatgggtg
     6421 ctcatgtaac tgttataaca atgggacctc ctcaagcaga tatggcttta aaagaagctt
     6481 tagcaatggg tgcagataga ggtatattat taacagatag agcatttgca ggtgctgata
     6541 cttgggcaac ttcatcagca ttagcaggag cattaaaaaa tatagatttt gatattataa
     6601 tagctggaag acaggcgata gatggagata ctgcacaagt tggacctcaa atagctgaac
     6661 atttaaatct tccatcaata acatatgctg aagaaataaa aactgaaggt gaatatgtat
     6721 tagtaaaaag acaatttgaa gattgttgcc atgacttaaa agttaaaatg ccatgcctta
     6781 taacaactct taaagatatg aacacaccaa gatacatgaa agttggaaga atatatgatg
     6841 ctttcgaaaa tgatgtagta gaaacatgga ctgtaaaaga tatagaagtt gacccttcta
     6901 atttaggtct taaaggttct ccaactagtg tatttaaatc atttacaaaa tcagttaaac
     6961 cagctggtac aatatacaat gaagatgcga aaacatcagc tggaattatc atagataaat
     7021 taaaagagaa gtatatcata taatttaagg ggggataaag aatgggtaac gttttagtag
     7081 taatagaaca aagagaaaat gtaattcaaa ctgtttcttt agaattacta ggaaaggcta
     7141 cagaaatagc aaaagattat gatacaaaag tttctgcatt acttttaggt agtaaggtag
     7201 aaggtttaat agatacatta gcacactatg gtgcagatga ggtaatagta gtagatgatg
     7261 aagctttagc agtgtataca actgaaccat atacaaaagc agcttatgaa gcaataaaag
     7321 cagctgaccc tatagttgta ttatttggtg caacttcaat aggtagagat ttagctccta
     7381 gagtttctgc tagaatacat acaggtctta ctgctgactg tacaggtctt gcagtagctg
     7441 aagatacaaa attattatta atgacaagac ctgcctttgg tggaaatata atggcaacaa
     7501 tagtttgtaa agatttcaga cctcaaatgt ctacagttag accaggggtt atgaagaaaa
     7561 atgaacctga tgaaactaaa gaagctgtaa ttaaccgttt caaggtagaa tttaatgatg
     7621 ctgataaatt agttcaagtt gtacaagtaa taaaagaagc taaaaaacaa gttaaaatag
     7681 aagatgctaa gatattagtt tctgctggac gtggaatggg tggaaaagaa aacttagaca
     7741 tactttatga attagctgaa attataggtg gagaagtttc tggttctcgt gccactatag
     7801 atgcaggttg gttagataaa gcaagacaag ttggtcaaac tggtaaaact gtaagaccag
     7861 acctttatat agcatgtggt atatctggag caatacaaca tatagctggt atggaagatg
     7921 ctgagtttat agttgctata aataaaaatc cagaagctcc aatatttaaa tatgctgatg
     7981 ttggtatagt tggagatgtt cataaagtgc ttccagaact tatcagtcag ttaagtgttg
     8041 caaaagaaaa aggtgaagtt ttagctaact aatttcttgg ggagggtatt atgagtacaa
     8101 gtgatgttaa agtttatgag aatgtagctg ttgaagtaga tggaaatata tgtacagtga
     8161 aaatgaatag acctaaagcc cttaatgcaa taaattcaaa gactttagaa gaactttatg
     8221 aagtatttgt agatattaat aatgatgaaa ctattgatgt tgtaatattg acaggggaag
     8281 gaaaggcatt tgtagctgga gcagatattg catacatgaa agatttagat gctgtagctg
     8341 ctaaagattt tagtatctta ggagcaaaag cttttggaga aatagaaaat agtaaaaaag
     8401 tagtgatagc tgctgtaaac ggatttgctt taggtggagg atgtgaactt gcaatggcat
     8461 gtgatataag aattgcatct gctaaagcta aatttggtca gccagaagta actcttggaa
     8521 taactccagg atatggagga actcaaaggc ttacaagatt ggttggaatg gcaaaagcaa
     8581 aagaattaat ctttacaggt caagttataa aagctgatga agctgaaaaa atagggctag
     8641 taaatagagt cgttgagcca gacattttaa tagaagaagt tgagaaatta gctaagataa
     8701 tagctaaaaa tgctcagctt gcagttagat actctaaaga agcaatacaa cttggtgctc
     8761 aaactgatat aaatactgga atagatatag aatctaattt atttggtctt tgtttttcaa
     8821 ctaaagacca aaaagaagga atgtcagctt tcgttgaaaa gagagaagct aactttataa
     8881 aagggtaaaa atctaattaa aaatatattt gctgttaact agattaaata gtattttaat
     8941 ctagttaaca gaatataaat actaaaataa caggaggtta taactatgaa attagctgta
     9001 ataggtagtg gaactatggg aagtggtatt gtacaaactt ttgcaagttg tggacatgat
     9061 gtatgtttaa agagtagaac tcaaggtgct atagataaat gtttagcttt attagataaa
     9121 aatttaacta agttagttac taagggaaaa atggatgaag ctacaaaagc agaaatatta
     9181 agtcatgtta gttcaactac taactatgaa gatttaaaag atatggattt aataatagaa
     9241 gcatctgtag aagacatgaa tataaagaaa gatgttttca agttactaga tgaattatgt
     9301 aaagaagata ctatcttggc aacaaatact tcatcattat ctataacaga aatagcttct
     9361 tctactaaga gaccagataa agttatagga atgcatttct ttaatccagt tcctatgatg
     9421 aaattagttg aagttataag tggtcagtta acatcaaaag ttacttttga tacagtattt
     9481 gaattatcta agagtatcaa taaagtacca gtagatgtat ctgaatctcc tggatttgta
     9541 gtaaatagaa tacttatacc tatgataaat gaagctgttg gtatatatgc agatggtgtt
     9601 gcaagtaaag aagaaataga tgaagctatg aaattaggag caaaccatcc aatgggacca
     9661 ctagcattag gtgatttaat tggattagat gttgttttag ctataatgaa cgttttatat
     9721 actgagtttg gagatactaa atatagacct catccacttt tagctaaaat ggttagagct
     9781 aatcaattag gaagaaaaac taagatagga ttctatgatt ataataaata agaatttggg
     9841 aattaaaagt ttaaataaaa ttgtttaaaa aacaatttcg gatatatgaa aaatctaatt
     9901 taatgggggt aatgaatatg agagaagtag taattgccag tgcagctaga acagcagtag
     9961 gaagttttgg aggagcattt aaatcagttt cagcggtaga gttaggggta acagcagcta
    10021 aagaagctat aaaaagagct aacataactc cagatatgat agatgaatct cttttagggg
    10081 gagtacttac agcaggtctt ggacaaaata tagcaagaca aatagcatta ggagcaggaa
    10141 taccagtaga aaaaccagct atgactataa atatagtttg tggttctgga ttaagatctg
    10201 tttcaatggc atctcaactt atagcattag gtgatgctga tataatgtta gttggtggag
    10261 ctgaaaacat gagtatgtct ccttatttag taccaagtgc gagatatggt gcaagaatgg
    10321 gtgatgctgc ttttgttgat tcaatgataa aagatggatt atcagacata tttaataact
    10381 atcacatggg tattactgct gaaaacatag cagagcaatg gaatataact agagaagaac
    10441 aagatgaatt agctcttgca agtcaaaata aagctgaaaa agctcaagct gaaggaaaat
    10501 ttgatgaaga aatagttcct gttgttataa aaggaagaaa aggtgacact gtagtagata
    10561 aagatgaata tattaagcct ggcactacaa tggagaaact tgctaagtta agacctgcat
    10621 ttaaaaaaga tggaacagtt actgctggta atgcatcagg aataaatgat ggtgctgcta
    10681 tgctagtagt aatggctaaa gaaaaagctg aagaactagg aatagagcct cttgcaacta
    10741 tagtttctta tggaacagct ggtgttgacc ctaaaataat gggatatgga ccagttccag
    10801 caactaaaaa agctttagaa gctgctaata tgactattga agatatagat ttagttgaag
    10861 ctaatgaggc atttgctgcc caatctgtag ctgtaataag agacttaaat atagatatga
    10921 ataaagttaa tgttaatggt ggagcaatag ctataggaca tccaatagga tgctcaggag
    10981 caagaatact tactacactt ttatatgaaa tgaagagaag agatgctaaa actggtcttg
    11041 ctacactttg tataggtggt ggaatgggaa ctactttaat agttaagaga tagttttaga
    11101 ttataatatt tacaaattaa atatttaatt atgaatttaa cctgtaagaa taacaaatag
    11161 ttattcttac aggtttttat agttaaaaat aattattatc tattaaacaa aaatagtgta
    11221 aaatatataa tatcatgatt ttgaagatat aaaaaagtag gaagatataa aagaaagtag
    11281 gtaaaagttt gtttaaaaaa gagaatcaaa gatataatct tatatcgtat acaaatgaag
    11341 aagaaatgac tttaaaagaa gttttattgg acaagctaaa tttctcagtt aggtcattat
    11401 caaaaatgaa gagggaaaag agtgttttag taaatggagt atataaaaaa ccgagtttaa
    11461 aggtgtatag tggtgattta atagaagtaa aaatatacga ggaaaaagca aattttgaac
    11521 ctcaagattt aaatttacag ataatttatg atgattttga tataattatg gtgaataaac
    11581 ctccgtttat ggtagttcac ccaacaaaaa gccactatga taaaacaata gcaaatggta
    11641 ttagttacta tatagataat caaaaagaaa acgtaaaaat aagatttgta aataggttag
    11701 atatgaatac ttcagggtta gttatagtag caaaaaatgc ttatgcacat catactttgt
    11761 ctacagcaat gagtgaaaat aaagttgaaa aaaagtatat aacagtagtt gatggaatta
    11821 taaaagaaaa tgagggaact atagatgagc caatttatag acctacagaa gattcaataa
    11881 agaggataat agatgaaaga ggacagtcat ctgttactca ctataaagta atagaaagac
    11941 tagaaaatgc aactgtatta gaagtaagct tagaaactgg tagaacacat caaataagag
    12001 tacatatggc tcatataggt catggaataa taggtgatga gttgtatggt tatgtagatg
    12061 aagagctaat aaataggcaa gcacttcatg catataagtt agagtttgaa caaccaagaa
    12121 caaaagaaaa attgaaattt aaagcagata taccagagga tatgaaagag ttaatatcga
    12181 aattaagata atggggtgat atatatgata atacataaat ttataataca tgttttggac
    12241 aaaaatagtg atgttccaat attaaatgac tttgaaggca aagttaatca agaagtagat
    12301 ggttttttcc agaaagctat aaaaagaatt gcaaaagatg aggatttaag aaaaggtgtt
    12361 tttaaagatt ataatgacaa tttaattaaa aactgttgtg aacaaataat atatgatgaa
    12421 agtacttttt tgaaaaattc taaagaaatt gcatcttact tgtttgatgt tatgaaaatt
    12481 aatgctgcac tagaatcttg tgatttggca atttgcttat acactattaa agatgaaaag
    12541 agtgtagcaa tactgaaatt agattataaa aagttgtaca ctcattcaat tgaatttgtg
    12601 gatgataaat ttaatattca aatggtttca aatgaaattg gtatcccaga gactttaaga
    12661 caaaagcaag gagctttgat tagtctaagt ggtataaatg atgagtttca tctaaggctt
    12721 ttagataagg atgctgaaaa agaagggtca gaatctaagt ttgtaacaga atttttaaat
    12781 gctaaaaaaa tagatgatga taagtacaag accaaggtat ttaaaaatac agctgaaaat
    12841 tggataacca atgctcttag taatgatata aagcaagctg aagatgtaag aagtatatta
    12901 aattatacac tgaaggaaaa acatgaagtt gatataaatg actttgttga taattcaatt
    12961 aaaaatgacg agttaaagga tagctttaaa gagcatatgg aagaaaaagg ccttgatgaa
    13021 agttttagta tagataaaaa atgggttgag aaaaagctta aaaaaagaag tataaaaacg
    13081 gataatggtt ttgacataaa agggaatcta actgattttg aagacccaat gaagtatact
    13141 gtaaaacaaa atcaagatgg aactatagat ataataataa agaatattac gttttatgaa
    13201 gaaaagtaat ttatatactt agtatataaa ttaaatataa gagcgtgtat tttatatatt
    13261 gtaaataaat gtaatctagt ctgtgcgaac tctaatataa tttattatgt agaagtttac
    13321 acagactatt tatatttgta gacaaaacga tactatttgt tttcttcgat atatttacat
    13381 atatcaccaa tacatttaaa gttctcagct tcctcgtctg gtatttctat tccaaattca
    13441 tcttcaaggg ccattataat ttcaacagca tctaatgaat cagcttctaa gtcgtccatt
    13501 aaagatgctt ccatagttat ctcatctaaa ttgtctaccc ctaattgttc tgctattatt
    13561 tcttttattt tctcaaacat tttaagcctc ctaaagcagt tttaattaat tttaatgtta
    13621 gtttaatata atatcaagaa tttttcaata tggaatctat aaaaaataat aaaattttat
    13681 aaatgtagta ataaagtatt atttgatgaa taaactttat tgataacaaa tataacttag
    13741 gaggaaatgc tatgaaatat gtaggactat tactaacttc agtaggaatg tttttactta
    13801 tagcagtaaa tttttattat aattcaataa ctttagatat gcaacgtata gaggattatg
    13861 taatggagac caatttaatc cttgaagatg tagctgaaaa ggagagttat gtatctaatg
    13921 aaaaagaaga ctacatatca aggttaatgc atgtaaagaa aggaatagaa aattcaaaaa
    13981 catccttctt gatagaaaga tataaggagt acaaaataaa atcaatagaa agtttaatct
    14041 atactatatc agaggagaaa aaagattatc tggatgaagt tgatagatac aataaacttg
    14101 gtgaaaaaga aataaataaa ctaatcaata aaaacttttt agaagtaact tatctgtcta
    14161 ttactacata tatttaatta aagagacaaa acttagtctc ttaatgattc aaggaggatt
    14221 agttatgaca aaattagaag acaatatgga acgtttcttt ggaggatgcg aaggattttt
    14281 tgggaataaa atgttgataa taatagttat agtattctta ctattatgca cagacatttt
    14341 agaagactta ttatgcgatg acaatatatg gatatgggtt atattaatag tattgttatt
    14401 atttaacttc gatgatggtt gttgttgcta aaaaatattg attattaaat aggtaaaacc
    14461 agtaacttat acaagttatg gttttacctt ttttagttca aattaaaaaa tttaaaaaaa
    14521 tagtaaaaat acattagcaa aatggaaacc aaatgcatag tatatgttag gagcgaaaag
    14581 ctcaacaaaa tatcaacagg aggtaacgta taatgttaga tagattcttt ggagaagaag
    14641 gatgttttgg tggaggagga tggtggataa tagtactatt cttcctattc ttagcttttg
    14701 gagatagctg ggcagaattt gacataatgg catggatacc attcctaata gttttattaa
    14761 tactttgctg ctgtggtgga tgcttagaat aagaacacag ttattaaaaa aaggagggct
    14821 ttgttatgtt agatagattt tttggagaag aaggatgctt tggtggagga ggatggtgga
    14881 taatagtact attcttccta ttcttagctt ttgaagattg ctggagagac attgacataa
    14941 tgtcatggat acctttctta atattattgc taataacttg ttcatgtggt ggattctttg
    15001 atggtgaagt aggttgcggc tgctaatact taaatcatta gaataaaaaa atgctggtat
    15061 tttataccgg cattttttta ttttattttt taatatgatt taaattaaga atgaatatta
    15121 ttaaatttgt tgaatatagg gtataatata aaatataatg tatttaaatg tacttgcttt
    15181 tatggtaaaa taaaaactta tataaatgta gttaaaatag gaaccttcaa aaatttggtt
    15241 cagttttaga taaatttagt aaaataagga ggatgaagat gaaaggcaat ataacgataa
    15301 aagatgttgc taaacaagca ggagtgtcaa tatctactgt atctagagtt ataaatgatt
    15361 caaaacctgt aactgatgaa gtcaaacaaa aagttttaga ggttataaaa gagactggat
    15421 atataccaaa tccacttgct agaagcttag taacaaagaa gagtcaatta ataggggtaa
    15481 tagttccaga agtttcagat tcttttgtta atgaggtgtt aaatgggata gaagaggttg
    15541 ctaaaatgta tgactatgat attcttttag cgaatacata ctctgataag gaacaagaac
    15601 ttaagagtat aaatctattg agagcaaaac aagtggaagg tatagttatg atttcgtgga
    15661 tagttgaaca agaacatatc aactatatac aaaattgtgg aataccagcg acatatataa
    15721 gtaaaactgc tagaaattat gatatatata cagtaagtac tagcaacgaa gaagctactt
    15781 ttgatatgac agagcatctt ataaagaaag gtcatgaaaa gatagctttt ataatgacta
    15841 gtaaagatga tactgtttta gaaatggaaa gacttgctgg ttatgagaaa gcactttcaa
    15901 ataacaatat agaattagac aagagtttga ttaagtatgg tggaactgat tatgagagtg
    15961 gatacaatag tatgaaagaa ctattagatg atggaataat acctcatgcg gcttttgtaa
    16021 caggtgatga ggctgccata ggtgctataa atgctatatg tgatgctgga tataaggttc
    16081 cagaagacat atcagttgca ggatttaatg atgttaagat agctagaatg tatagaccta
    16141 aacttactac agtatatcaa cccctatacg atatgggagc agtagcaata agaatggtta
    16201 taaaattaat aaataaagaa ttaattgaaa ataagaaaat agaattacct tatagaattg
    16261 ttgatagaga aagtgttaca gaaagaaaaa aataaaacta ttaaaatcaa tcaggtagta
    16321 ccttaggcac tacctgattt tttatgtcaa taatatcaaa tttaataata gattaactaa
    16381 aaataattat ctaattgctt aaatagttta tttttatcta ttcatgaggg ttagactttt
    16441 tagttttctt aaataaactt ttagattttt tttgttgact acctggtgta ttttttggtg
    16501 caggtgaatt tgacttacct ttagtaggtt tagccttctt agttttagct ggtttagtat
    16561 ctctttgatt ttctgcatct gagatttttt ccattatagt atccaatttc ttgcgttgat
    16621 tttcatctaa aaacgcttta aattttgata ctacttcatc tcttccatct aagtttttac
    16681 ctatttttat aagttcctcc ataagttcac tttctgattt tcctttgtaa ctatcagcca
    16741 tcttttctat tttatctttg tcaatatttt tttgtttagc cattttttct aatgactctg
    16801 catctaattt tttcataaaa aaaccccttt ctataaatat atattgaatt atattcatat
    16861 tatttatata tagaaataat attgaagtgg tgaattgtga gtgatatata atataaataa
    16921 aaattcttat agataatata tatgcactga gctttaaaaa tggaacatta aaatatgaaa
    16981 aacatacttt aatatagggc aattatataa tataaggggg aattgtcttg aataacttac
    17041 tatttttgat tggagtgatt gcattgagta atggtaatat ctctatattt gatgataaaa
    17101 ataaatcaaa aggaaataat agagttagaa aaaacaaaaa aaatgcaaat gataaactgt
    17161 cacagtctac acatggcaat ttaaggtttg atgcaaatga cataaaaaga ggtttaaggc
    17221 tcatggattt aagtgaagaa gatttagaaa tgggaatgga aataataaca agaactaaaa
    17281 aatacatgtc aagagatgaa agaaaaatac taataaaaat agaaagtatt ttagatttag
    17341 ttagaggaat aaaaaaatta aataatatag atatagtaga tatagaagat gaaacggatt
    17401 ttttcagaaa aatggatagt gatgataaaa agaacatgat gataaaggag attatagatg
    17461 tattcccaga aaaaagaaaa aattcagttg aaaaagctat aggaatgaaa aagaagatag
    17521 atttatttgc agaattattt ttaccagatg attttggtga aggcggtttt agtttgagct
    17581 ctttggcaaa cataaacaat cttgggagta tgaataatct caaattgtta ggaagtcttt
    17641 taagaggtga tgatagtaat aatgatgatg aagacgataa tgaagagtta gattatgaag
    17701 atgaggaatt agattgtgaa gataaagaat tggattatga ttatgatgag ttaacagacg
    17761 aaggcgaaat tgaggctaat gaagatgaat tagtagaaga taagaacaca ggctatgaaa
    17821 atatagaaaa taatgaagat atagtatatg atgaggaact atacaacatt aatgagcaag
    17881 aatccgaaca caattataat gaaaaaaata ctaaaactag agctaagaaa aaacgataat
    17941 taactaaata cataaagaaa aaaggaagaa aaagtaatga tagaataaga ttatctcaat
    18001 tactatataa aaccacttta aaaaattcaa aatttattat ataataataa tatgaaatcc
    18061 tgaaacatac gataaggctt ttttaaattc aacctacaaa tgaaatacgg gagaccgtgt
    18121 ctggtaaatt ataacatgcc tagttagttt ggacgttaaa ggttactggc aagtcaccca
    18181 cctgggagtg cctagggtgt gaatttactt agccaccggt gtttcgggac aaaaaaagtg
    18241 ctactatttc aaatagtagt acttttttta ttaaaatgca tttttctact aaatacgcct
    18301 aattattgat aaatttaaaa agcaagtaac aattaagatt ataaaagcat agtattaatt
    18361 aggcttcaaa aagccaaaga cttaataaga atcgcttaca gggagggcaa tgaaaaatgc
    18421 aagattataa aaaaaataaa agaagaatga tgaatcagcc aatgtctaca atgaatgaag
    18481 aagaagtgta tacagatgaa ataaattcag aagacatgag agggtttaaa aaatcacacc
    18541 atcataatgg atgtaatact gataataagt gtgagtgcca tgatgagtgt aatccatgca
    18601 acccatgtaa tccatgtaaa cctaacccat gcaatccatg caaacctaat ccatgtgatg
    18661 acaattgtgg atgccatgac aattgtaaat gtgattgtga accatgtgaa atggattcag
    18721 atgaatgttt tgaaaacaaa tgtggaccag aatgctgtaa tcctatatct ccaagaaact
    18781 tctctgtatc aaatgcagtg ccatttgcaa tagaggctaa tagaatattt gatactatgc
    18841 aattccaaac atttacagat gcaacaggac caaatggaga gccattaact tttgaaacag
    18901 aagtagtaga agtatttggt tcagttccaa gtgcaggtca agcaagtgta actatagaaa
    18961 aaatatgctt aagtaatgat ggaatagtta tagacacagg aatgacaact ttagaagatt
    19021 tcgatttaga cccattagga gatatagtag gaagaaactg tgaaacaact tttgaatttg
    19081 cagtttgtgg agaaagaaac gctgagtgct gtagacaagg aaaaggcaaa tcagtagctt
    19141 ataaacaaag aggattaact gtagcagttc gtaatttagt actagagcta agaggtagat
    19201 gtggatgtac agagttcgtt gcattagctt tcccagcagt tagagcagga ggtggatgta
    19261 agagaagagt tgattatgta gaatttactt ttaacacact ttcagcacca atatgcttgc
    19321 cagctgacgg aagagctgtt actttaagac aagaatatca aactaactta actgtagatt
    19381 gtataggaaa atctatatta aaattagaat gcaacgaatg ttgtgaacct ttctatgaat
    19441 taattatacc aaatgatata gatttagtac tttgcttaca agaaacagtt agcacattaa
    19501 taagtgaaca aatagtagtt ttagcatcac caaatccaat ccaaccaaga cttgttgata
    19561 ctttctctaa agtatgtgat ttttcgcaat gtggacctaa tcatggaagt ggaaagccaa
    19621 gttgccacag atagataggt aagatatata gacagtttaa attataaata aaaatcatct
    19681 aaggaaggct accaaagtgt ataatttaca ttggtagcct tcacgttttt ataactagcg
    19741 tatagatttt agtgatttaa aaagcactat agtatgtaat agacaagtaa agattgatac
    19801 tatcgtcatt gtgtatatgt atgcataaat attaagtcct ggaattggaa gaaatacata
    19861 taaagctatt agttgaatta acatgccagt aatagtattt atacttgaag ctaattcttt
    19921 tcttattgaa tgtaaaatgc cagatagagt ttgatttagg gacataaata gtggaactaa
    19981 aaacatagct tttagatatt ctcctgcaag tgtattttta aatactaata tacataaatc
    20041 ttttccaaag aaataaaaaa atattgatga tagtatgcct acaaataaag taagaagtat
    20101 ggagtatttg atttttttaa ttacactctt acgctttctt agagccattt cctgtgaaat
    20161 agttggtatt aaatttacaa taagcgctga accaacagta aaaggtaaaa atataaaagg
    20221 cattaccatc ccactgacaa taccatacat acttaaagat tgttgataag tcattcctga
    20281 taatgctaat cttgatggta tcatcataga acttatagat tgcaaaagta tgtttgacat
    20341 tctattacaa gttatgggaa tagacatttt taatgtttcc atagaagaat tataaaaatc
    20401 ttttatgctt ataatatacc tattgtcaaa tgatgaatct cgccataagc aaataagcat
    20461 aaatataata ttactcaact caccaatcga tatacctaat agagctatat aacaattcat
    20521 agatttatta tttatgtaca taacaagtaa aaaaacaaat aatattttac caatttgttc
    20581 aatgatttgt ccaatagcag ggatttttac attctttatg ccatagtaat atcctcttag
    20641 tacgtttgaa agagttatta taactatagc aggacaaaca gctagtataa atagatttaa
    20701 tttagcatct tttaaaaatt taagtgataa aaaagaacta ttgaaagatg caactataga
    20761 tataattagt gctacgaaaa atgatatata taaagttgat ataaataaag cattagtatt
    20821 gtgtctatcg tttaaagctt ttctctttgc aactagacaa ctaagtgcag taggtatacc
    20881 tgttgttgta acagctaagc aaatcatcaa aaagttaaaa atcatatgat agatacctaa
    20941 acctgtttca cctagtgctc ttgataagaa tattttatat aaaaaaccaa atattcttac
    21001 aataaaattg gatataaata aaataactgt tgatagtact aaataaggta ctttcaaaaa
    21061 aatcacctcc ttatctttta aatatattca caaggaggtg taaaaattta taattttata
    21121 aattctggtt tcaatttatt aaatttagat acatatttaa atcccatatt ttttagtaat
    21181 gaatatatgt agtcaaactc acatgcaact tgacttatat gatgtgaatc tgaacctgtg
    21241 gttatgattt cccctcccaa gtctttatac atttgaagta tatacttaga tggggttagg
    21301 tctggaagat tatatctata acaagaggta tttatctcga tacctttgcc atcataaata
    21361 gcttgtttaa gagtttcttc aattatatca gaaaatagtc tatcatctaa aatagtgtca
    21421 taaggtgcat accttttaat caaatctaaa tgacctaaga cagagtaatc tttataattc
    21481 ttaacaatat tatataaata aagatagtaa ttttcataaa cttcatgttg agttttatct
    21541 ttaaaataat taccaaggta taagtccatt gtatctatag catggataga acatattata
    21601 aagtccaatg gatattgatg tgcttccttt ttgtatgtat ccattaattg agtttgaact
    21661 ccaaattcaa ttcctttttt tattgatatt ttatctttat atttgctttg aagagtctct
    21721 aaactcttaa aataatcttc gtagacaatt gaaaaagaat catcagcata tacatcatag
    21781 tcaacatggt ctgtgaaaca aatttcctcc aaaccgagtt caatagattt ttttaccatt
    21841 tcttccaatg tagattttcc gtctgtagag aaacttgagt gcatgtgata gtcgtaaaac
    21901 ataaatattt ccccttttta taaaaatttt ggtattatat atacatacta aataatatca
    21961 aatatacata aaagcatcaa taaaatataa atacaatgag cggtgataaa tttggaaaaa
    22021 tacagaatag atttgaatga gaaaaaatca actaaatata tacagatatt taatcatata
    22081 aaagaattga taataaataa agagttagta gaacatgaaa aattgcctcc aataagaaaa
    22141 ctatcaaatt ttttacaagt aaataacact actatagtta aggtatacga gcttttagaa
    22201 aatgaaggat atgtatataa aattataggg agtggtactt ttgtatcaag tttaaattct
    22261 tataaaagcg gtatagataa caggaaaaat aagattattg tgcaaagaga agatttaatt
    22321 cattttgaca atgggaatcc atctaatgat atgtttccta tcgattcatt taaaaatgct
    22381 gtaaatatgg cattatctaa agatggtagt tcaatatttg aatatgatga aggtcttggc
    22441 tctgaagaat taagagaaaa aatggtagag tatctaagtg atggaaatat aaaaactact
    22501 aaggaaaata ttcaaataat atcaggtgct caacaaggta tagatatagt ttgtaaagga
    22561 cttataagtt attcagatgt tgtttttatg gaagaacctt cttacaatgg agcaattgaa
    22621 gtgtttaaaa gtagaggtgc aaaaataata tctataccta tgttagatga tggaattgat
    22681 ataggtattt taaaattaaa gttggaaaaa ataaaaccaa gacttatata cattatgcct
    22741 aattttcaaa atccaactgg aatatcttac tcaacatata aaaaaaagaa attgatagaa
    22801 ttggcagaag aacatgattt ttatataata gaagatgatt ttataagtga ttttgttttt
    22861 gaatcaaagg acaatagaac aattagaagt tatgatgata aaaatagagt agtatatata
    22921 aaaagctttt caaaaatact tatgccaggt cttaggatag gaattgtaga aatgccaaat
    22981 gagttgttaa aaaaagtttt atgggctaaa tattcatcag atatatcaac accaggttta
    23041 attcaaaaat caatgtatta ctatatgaat aattttgatt ggaagaatta tttgagtcat
    23101 atagagaaga tatatactga taagtataaa ttggcaaaaa acttgattaa tgacaaattg
    23161 agtggtaaat taaaagttag aaaatcttgt ggaggaataa actttttttt agaattacca
    23221 agaggatata catctcaagc atttacaaat tttatgttaa acaaaggagt gtcaatgctt
    23281 ccagggactt atttttttga caatctagtg gatgatagat tctttaggat aaatatagct
    23341 cgcccaagca tggaagaact agaaaaggga atatctataa taagtgataa tatagatgag
    23401 tttttttgtg aatataaaaa caagttagat ataaaaagca ataaattatt ttattaaata
    23461 aatagaggtt ttttaggagg aaaaaatgtt tgacaagaag aaattagata ggattaatga
    23521 attagcaaag aaaaataaag agggaatcct ttctgctgat gaaatcaaag aaagagaaat
    23581 tttaaggaaa gaatatttag aaaactttag agcccacttt agatcaagac tagatagtgt
    23641 caaagtagtt tcaccagaag aatatgagca atacatgaaa aacaataaaa attagaactt
    23701 agtaggagga aaatatgaac ttaaagtacg aatttaagaa tgcttgggat tttgaaagaa
    23761 aagaaaatac tattgaacaa attatgcaat attctagcaa ttatatggag tttttaagta
    23821 agtcaaagac tgaaagactt tcagtaaaag aaattataaa attggctaaa gaaaataact
    23881 atatttcaat agatgaagct atggaaaaag gaagcataag ttgtggtgat aagatttatg
    23941 ttataaataa agagaaggca gtagcacttt ttgtaatagg taaaaattat atagaaaaag
    24001 gtatgaagat tataggaagc catatagatt cgccaagatt agatttaaaa ccaaatccac
    24061 tgtatcaaga gagtaatcta ggttttttta agactcatta ttatggtgga ataaaaaaat
    24121 accaatggac ggctattcca cttgctttac atggtatagt tatattaaat gatggaacaa
    24181 aagtggacat atctattggg gaagaagata gtgacccagt attttgtgta actgatttat
    24241 tgattcattt ggctggagac caaatgcaaa agaaactatc agaaggaatt agtggagaag
    24301 ctttaaatat attaattggg aatatgcctt tagaagatga agaaaaagaa cctataacag
    24361 caaatatatt aaagatttta aatgagaaat acaatatagt tgaagaagat ttattaagtg
    24421 ctgaaattga agtagttcca gctggtaagg ctagagattt agggcttgat aggtctatgg
    24481 tgttgggata tgggcatgat gatagggtat gttcatatgc tgctgtaaaa gctatactag
    24541 aaactgaaca accagaattt acttcagttg cattatgtgt agataaagaa gaaattggtt
    24601 ctaaagggaa tacaggtatg cactcaaagt tttttgaaaa tactgtggct gaactgattg
    24661 ctttagaagg agattattgt gatattaaag taagaagagc tttagcaaat agtaaggtat
    24721 tatcagctga tgtttctgct ggatatgacc caaattttgg agaagcatat gaaaagagaa
    24781 attcggcata tatgggaaat ggagtagttt taactaagta tacaggttca agaggaaaga
    24841 gtggatgtaa tgatgctaat gctgaattta tgtcagaagt aaagagaatt tttaataaag
    24901 gaaatgtagt atggcaaact gctgaacttg gaaaagtaga ccaaggagga ggcggaacta
    24961 tagcccatat attggcaaat caaggtgcag aggttataga ctgtggtgtt ggagttttaa
    25021 atatgcatgc accacatgag attgtctcta aagtagacat atatgaaatg tataagggat
    25081 ataaggcatt ttttaatata aatttataag taaaaatata aatgtaataa gtgaataaag
    25141 gctgttgtat aaacaattta atctgttgta caacagtctt atttttatgt aaaaatagca
    25201 atttgtcaca ctagagaaaa taaaagaaaa tactgtcaaa ttaaaaatta tttaaaaaaa
    25261 aattgacaac gatttcatct tgttataata tgtaataaag aacttgttat ataatgttat
    25321 aaaaatgtta catagggagg tgagcaaaat ggatgaaaag agacaaaaaa aagaaatgga
    25381 tttagaagta aattcctttg ttcctttata tcaacaacta tatgacaaca taaaaaaaca
    25441 aatagcatct ggcatatata aaccaggaga taaacttcca tctgaaggag acttatgtaa
    25501 agaatttaat ataagtcgta taactgtgag aaatgcactt aatgaacttg taaaagaaga
    25561 tatattatgt aaaaaacgtg gtaaagggac ttatgtaaca ataccagaga gaatagaagc
    25621 aacatgtgca ggaaatagtt ttactaattc ttgtcatcgt attaatgcaa aaccaagtac
    25681 caaaataatc tctgttttaa taaaaaaagc agataagcaa gtagctgaag ctttaagtat
    25741 tgaactagaa gagaaggtaa tatgtatcaa aagacttaga ttaatagatg aagtacctgt
    25801 tatttttgaa gttgactatt ttagaataga ttacatgttt ttattaaaag aagaattaga
    25861 agataaatct ttaatggagg ttatatctaa taatataagc acattaccta aacgagtaga
    25921 aaatatattt gaagtaaaac attcaaacaa agaatattct gaccatctta aatgtgcaag
    25981 taatatgcca ctattgaaag taagacaatc ggtgtacaca gaaaataatg atgtcttata
    26041 ttacaatgag caatttataa gaagtgataa gtacaaatat gcagtatcag cagaaatata
    26101 atctataatt tagtaggagg atgagtatga aaaagataat attagcatct catggagact
    26161 tttcaaaagg gcttttaaat gcagtaaaaa tgatagtagg agatttagca gattgtgtca
    26221 gttcgtatgg tttgtaccct ggacaaagtg cttcagattt tgctttagaa ttagaaaaaa
    26281 caatatcaga agataaagaa tgtgaatata taattttatc tgacttatat ggagcaagtg
    26341 tatgtacagc aatgttaaga ttaactaatc tacataatgt taggttattt tctggaatga
    26401 atttgaatat ggtattagaa cttcttacaa ggttttctga tgaattaaca gttgaagata
    26461 tagaccaatt agtggaagaa tctagaagag gaatacaaag cgtgacatta caaattaaag
    26521 aagaagaaga ggtattttag tttatgaaaa aaaagggaat attatcagta tttaaaagta
    26581 aaaaaccaat tatagcaatg atacacttaa aaggagatac tccagaagat atctttgaaa
    26641 gagctaaaaa agagattaca atctttgaag aaaatggtgt agatggaata atgttagaaa
    26701 attactatgg taattattat gatttagaaa gaatacttga atatgtatca aaagctaatt
    26761 tatcaatacc atatggagta aactgtttaa atgttgatac tatgggattt gaactggcaa
    26821 caaaatataa tgctagttat atacaggttg attctgtagt aggtcatgtt aaacctagag
    26881 atgaagctac tttagaagag ttttttaaat tacaacgttc aaaatgccca gcgtatttaa
    26941 ttggaggcgt acgttttaaa tatcagccag tattatcaga aaatgatgtt gaagaagatt
    27001 taaaaatagg aatgacaaga tgtgatgcta tagcagttac agaaaatgct acaggtcaag
    27061 aaacatcaat ggaaaaaata gaactgtttc gtaaaaattt aggagacttc ccattggtta
    27121 tagctgctgg agttacatta gaaaatgcaa aaaaacagct taaattagga gatatggcaa
    27181 taataggaag ttactttaaa gataattaca aagattttgg ggatgttagt gtagaacacg
    27241 taaaaacatt catggatgaa atcaaaaaaa ttagggagga attataatga ttaaattagt
    27301 aagagtagac cacagactta tacatggaca agtagcattt acatggacaa agtttttaag
    27361 cacagactgt atattgatag caagtgatga cttattaaaa gatgaattga gaatggcagg
    27421 acttaaaatg gctaaaccat ctaatgttaa gttagtaatg aaaagcatag cagattctat
    27481 aaaagcactt aactcaggtg ttactgataa atacaactta ttgatacttt gtgaatctgt
    27541 agaagatgtt tatagacttg ctaaagaggt aaaagctatt aaatctataa accttggtgg
    27601 aacaaaatca gatgataatc gtgaaaatat atctaaagca gtgcatgtat caaaagatga
    27661 tataaagatg attaaagagt tagattcaga aggcgtaaat gtatttgtac aattagttcc
    27721 tgatgatgat gctacaaatg taatgaaatt aatataattt tagggggatg aaaatggaat
    27781 ttacacaagt tattttgata actctaattg cattctttgc atatatgcat agttttgtag
    27841 gttctacaat gcataataga cctatagttg ttgcaccact tgttggttta gcactaggta
    27901 atcttcatac aggtatagta ataggttcaa cattagaatt agtatttatg ggtgcatttc
    27961 ctgttggggc aagtaatcct cctgattttg tgtctggttc tattattgcc acagcgtttg
    28021 taatattaac gggtcaagat gtatcagcag cagttgtact tgcagttcct atagcaactc
    28081 tagtattgct tatagataac ttcttaatga cagtagtact tacatgggga gctcatattg
    28141 ctgaccgtta tgcagaagag gggaatattg aaggcgtaga acgtgtgcaa ttattatttg
    28201 gtataggaaa taaattaata ttagcaataa tagtaggtat aggattttca ttaggtgttc
    28261 cagtaataga aaaaatactt tcatttatac catcatatgt aactcatgga atggatgttg
    28321 cagcaggcgt gattccagca ataggttttg ccatgttagc tagaatgatg cttaataaga
    28381 aaacagtagc atttttatta ttaggattta tattggttgc ttacctaaac ataacagtaa
    28441 caggagttgc attgtttgga ttagcaatag ctttaatata tgttaacttt gtaggagaga
    28501 aggaggtaat agtagatgac aacgagttct aagaagttag agacaatttc accagatagt
    28561 aaaataacga ggaaagactt ttggaaatgt tttagaaggt cattgacatt agattcatct
    28621 tggaactacg aacgtatgca aaatatagca tatgcataca tgatggcacc aataatccgt
    28681 agattatata aagatgataa agaaaagaaa tctaaggctt taaaaagaca tttagaattt
    28741 atgtcagtta ctcctcatat aagtactctt ttggtaggta tatctggagc tatggaagaa
    28801 gaaaatgcaa agaataaaga atttgatgct aatagtataa atgctgttaa atcaagttta
    28861 atgggtccag tttctgggat aggagattca tttttctggg gaacattaaa gcttatagca
    28921 gctggggttg gtatagcttt agcttctcag ggtaacataa tgggaccgat tttattcctt
    28981 ttaataataa atgttcctca ttttattata cgctatatat gtctagataa aggatttaag
    29041 tatggaactc aattctttaa agatgtgagt ggttcaagta tagtttcaaa agttatggaa
    29101 gcagcttcaa tgttagggct tatggtaatt ggaggtatga ctgcatctaa tgtaatgtta
    29161 aaacttagtg ttaatgtagg tagtggagaa tgggcagagc ctattcaaac ttacttagac
    29221 caaataatgc catgtatgct tccagctatg atattcggta ttatgtattg gttattagga
    29281 aaaaaggtaa agacaactac gatattaatt tcagttatga ttatttgtat agtattggct
    29341 gctattggag tagtataaat aaaaaaatcc caagttcatt tgaacgaggg attttttgac
    29401 taaattaaat aaaaaagaaa gatggttcat aataatttat aagcatttat gtgatagtta
    29461 taattagctt attaattagt tataaactca aaattaagca ctactttatt aaagttatta
    29521 tattataatt tcgaaatact cacataatct gtttagaaat ttatcttcta atggggaaaa
    29581 agttttcttt ttagagtaaa tgaagaaaaa ctttctagta aaatcaacat cctttatttt
    29641 ataacatttt attttacctg cattgagata atcgatagct gaaatgtacg atataaagga
    29701 tactccaaga cctaatctaa ccatttcctt aatagcctca tttgattcta catgagcgat
    29761 tacatttaat ttacttactg gaaagttatt tttactaagt gtgtttagta ttaagtttct
    29821 tgtacctgac ccttcttttc tcataataaa atttagataa gccaattctc ctatatcgat
    29881 acaattattt ttattatcaa ttttaaaatc tgaaggagtg ataagaacaa gttcatcatc
    29941 taataagtct aggtactcta tttgaggatt attgatttta gaaccaacta atccaaagct
    30001 tattcgttca tttaaaattt ctgatatagc atattgagaa tcataatggc ttatggaaaa
    30061 ttttacatct ggataactca tagaaaaact cttcataaaa tcaggtaaaa tataagtttc
    30121 aggtatggaa ctacatgcta tatctatgat accttctatt tttcctgaat attcttttat
    30181 atcataaatt gcacgtttgc aattattcaa tatatatata gcatgttcat aaagaatttc
    30241 accagatttt gtgagtgtaa taactttgtt gcttctattt ataagaacag tttctagctc
    30301 tctctctaaa ttctgtatgt gtgcgcttac agtaggttga gttaaaaaaa gttctcttgc
    30361 agcctttgaa aaactctgat gttttgcaac agcaacaaat acttcaagtt gtttaaaatc
    30421 cattatttaa tcctccttaa attatctaaa actattataa catatttgat aaattaatag
    30481 tcaaaggtga tttttaaaat agaaaacctt taaatattca tggagttttt gattgtttag
    30541 gaataagttg gtataatgtt gtaaggtact tacaataaaa atatcgtaaa ttgaattttc
    30601 aaaattcaac tttaaagtgt tttttattac agaaaaatta caattagctt cttaatctta
    30661 ttaaattcaa ataaatatta tataattatt ttataatata a
//
LOCUS       DAFKMX010000023        29747 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFKMX010000023 DAFKMX010000000
VERSION     DAFKMX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.023"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            646..2316
                     /locus_tag="LZB08_000920"
     CDS             646..2316
                     /locus_tag="LZB08_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBM7314434.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKYQLSK"
     gene            complement(2438..3172)
                     /gene="pflA"
                     /locus_tag="LZB08_000921"
     CDS             complement(2438..3172)
                     /gene="pflA"
                     /locus_tag="LZB08_000921"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBM7314435.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3332..5563)
                     /gene="pflB"
                     /locus_tag="LZB08_000922"
     CDS             complement(3332..5563)
                     /gene="pflB"
                     /locus_tag="LZB08_000922"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBM7314436.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5856..6917
                     /locus_tag="LZB08_000923"
     CDS             5856..6917
                     /locus_tag="LZB08_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBM7314437.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7008..8621
                     /locus_tag="LZB08_000924"
     CDS             7008..8621
                     /locus_tag="LZB08_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7314438.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8896..10881
                     /locus_tag="LZB08_000925"
     CDS             8896..10881
                     /locus_tag="LZB08_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBM7314439.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIK
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKVGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            10886..11320
                     /locus_tag="LZB08_000926"
     CDS             10886..11320
                     /locus_tag="LZB08_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBM7314440.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11349..11909
                     /gene="srlA"
                     /locus_tag="LZB08_000927"
     CDS             11349..11909
                     /gene="srlA"
                     /locus_tag="LZB08_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBM7314441.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            11973..12341
                     /locus_tag="LZB08_000928"
     CDS             11973..12341
                     /locus_tag="LZB08_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBM7314442.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12366..12932
                     /locus_tag="LZB08_000929"
     CDS             12366..12932
                     /locus_tag="LZB08_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBM7314443.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            12973..13338
                     /locus_tag="LZB08_000930"
     CDS             12973..13338
                     /locus_tag="LZB08_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBM7314444.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13439..14212
                     /gene="srlD"
                     /locus_tag="LZB08_000931"
     CDS             13439..14212
                     /gene="srlD"
                     /locus_tag="LZB08_000931"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBM7314445.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14561..16915
                     /locus_tag="LZB08_000932"
     CDS             14561..16915
                     /locus_tag="LZB08_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7314446.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEKSLKNTSNKVNYASDELDMALHKVNYVSDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMTSNKVNCVSDELDMTSNKVNYVSDELDMT
                     SNKVNYVSDELDITSDKTDYISNELYNLSNKSITDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKGIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            17003..17338
                     /gene="spoIIAA"
                     /locus_tag="LZB08_000933"
     CDS             17003..17338
                     /gene="spoIIAA"
                     /locus_tag="LZB08_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBM7314447.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17352..17780
                     /locus_tag="LZB08_000934"
     CDS             17352..17780
                     /locus_tag="LZB08_000934"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBM7314448.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17803..18567
                     /gene="sigF"
                     /locus_tag="LZB08_000935"
     CDS             17803..18567
                     /gene="sigF"
                     /locus_tag="LZB08_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBM7314449.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18847..19284
                     /gene="spoVAC"
                     /locus_tag="LZB08_000936"
     CDS             18847..19284
                     /gene="spoVAC"
                     /locus_tag="LZB08_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBM7314450.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19451..20464
                     /gene="spoVAD"
                     /locus_tag="LZB08_000937"
     CDS             19451..20464
                     /gene="spoVAD"
                     /locus_tag="LZB08_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBM7314451.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVCTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20478..20840
                     /gene="spoVAE"
                     /locus_tag="LZB08_000938"
     CDS             20478..20840
                     /gene="spoVAE"
                     /locus_tag="LZB08_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBM7314452.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21023..22039
                     /locus_tag="LZB08_000939"
     CDS             21023..22039
                     /locus_tag="LZB08_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBM7314453.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22414..23256
                     /locus_tag="LZB08_000940"
     CDS             22414..23256
                     /locus_tag="LZB08_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBM7314454.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23271..23735
                     /locus_tag="LZB08_000941"
     CDS             23271..23735
                     /locus_tag="LZB08_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314455.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23722..24894
                     /locus_tag="LZB08_000942"
     CDS             23722..24894
                     /locus_tag="LZB08_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7314456.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            24938..25873
                     /locus_tag="LZB08_000943"
     CDS             24938..25873
                     /locus_tag="LZB08_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBM7314457.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26148..28841)
                     /locus_tag="LZB08_000944"
     CDS             complement(26148..28841)
                     /locus_tag="LZB08_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBM7314458.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            29055..>29747
                     /gene="yunB"
                     /locus_tag="LZB08_000945"
     CDS             29055..>29747
                     /gene="yunB"
                     /locus_tag="LZB08_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBM7314459.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTN"
BASE COUNT        11259 a         3561 c         5168 g         9759 t
ORIGIN      
        1 aatcagttga aaatgtaata ttagtaaata gtacatcaat cctatatacc ctgtaagcaa
       61 ctctattttc atgtactaaa aatgtaccta ttttattaac tgaaaagaat aaattaaatg
      121 taagtacata agaagataga aaaaattaaa aacaaaaaat atttatataa ataaatataa
      181 aaaatgatat aatccctatt aaaaaattta tagatgatga agaaatatca aaatcttata
      241 ttataggaag agacacttct ttgccaaaat ctatagaaaa acaataccaa atccaaagag
      301 aataggcgga gaaaaaaata aaataaattc aagagtaata gaaaagtttt atagaataaa
      361 gaactgataa ctctttatat aacgaaaaat agaattttaa gagaaaatga tttaataaat
      421 gatttaacat ggtgttttag caggtaaaaa taaatcacca gtaatgatta tagaaaaatt
      481 actttatgaa tctcaaaaaa cttagttaag agtaagagat ttgataagat aactaaagtt
      541 ggggaaatgt gtttgatgag acaaaaaatt agtaaaatag tataatctag ataaggatat
      601 tttgttgatt aaactacact aatagtaata ttgtaggggg aaaagatgag agaagaatcg
      661 aactctttaa gtttagacca tatagaaatt ttctttgaac ttttatctca aagtacagaa
      721 gattatatat ttttttggga tataaatagg aataaattta aaatttcttc agcaattttt
      781 gatgaattta acttaagtaa agagatagaa tctgatttgg tcaattgttg gagtaaaatt
      841 gtttatccgg atgatgttca aatatggaaa gatgatatac aagaattact acatggaaaa
      901 aaaggtgagc acaatttaga atataggctg attaataaat atgaagaaat agtatggatt
      961 tcttgtagag gtaaagttta tgtgtcggat gacccaaaaa ctatatttct agtcgggaga
     1021 ataaagaata ttggtgaaaa aaataaattt gattctatca caggaacgtg gaatagagaa
     1081 caatttgaac atagaatgaa ttatcttata aaagagaaaa tttataaaaa tggtgctatg
     1141 tttattatgg atattgataa ttttaaaaat ataaatgaaa aatatggaca ttcttatggt
     1201 gacaaagtat tgcgtgcaat tgctactgaa gtattagaat atcttccaaa agatgtaaga
     1261 ttgtataggt tagatggaga tgaatttgca tttttttacc ctatgtgtac taaagaaact
     1321 atagagaaaa tatatgaaaa gatacagatg tatacaaata ctcagcatga aattgaatca
     1381 aataagtact attgcacagt gactgcaggc gttgctatgt atccagaaga tggagataat
     1441 tatttagatt tatttaaaca tgctgatatt gcattggata tagcaaaaat tagtggtaaa
     1501 aatagaatta agtttttttc acaagagctc tatgaaaata aactaaaggt aatttcaatg
     1561 caacaaaaac taagagagtg cgttgaaaat aattttaatg actttgagct gttttttcaa
     1621 ccccaggtaa atgctgttac aaaagaggta ataggggcag aggtactttt aagatggcat
     1681 tcgagtactt atggagaagt atctcctgtt gaatttatac caatactaga acagagtaat
     1741 ttaattatac cagttgggaa gtggattata aaagaagctg taaaacagtg taaagaatgg
     1801 cataaaataa atccagactt taaaatatct gtaaatgtat cgtatattca gttaaaggaa
     1861 gattttttta gagattttat agtagaatgt ttagtagagt atcaattaag acctgaattt
     1921 ttaattttgg aattaacaga gaattgttgg ataccagata taaatttact aaatgataaa
     1981 tttattagtt taaagggtat aggagtatat attgcaatag atgactttgg tacaggatat
     2041 tcatctttaa attaccttaa agaactgtct gttaatataa taaaaattga aagaagtttt
     2101 gttaagaata ttacatataa tagttatgaa tatacttttc ttgaatatat tattaaatta
     2161 gctcatatta taaatttaaa agtttgtgtt gagggaatag agtcttatga agagtatgat
     2221 atagtaaaaa gcttaggtgt agatattatt cagggttttt tatttggaag acctgtaagt
     2281 gcctctgaat tttataaata tcagctatca aagtgatgta tataaaatga gatatatagt
     2341 atataaaaat agggcaaagt ggaagttttg aagtttatta aaaatttaca ctttacccta
     2401 ttttgttatg tcttctataa aggattaata tactacacta actattatat actttcactc
     2461 caaatttaga gattatttca ctagcttttt ggacatcttc cttagtggca tcaggaacat
     2521 cttttagttc ataatcgata cctaaacttt cccatttgtg tattcccata gaatggtatg
     2581 gtagcaattc aaatctttct acatttttca atgtagctac aaattgacct agtttttcta
     2641 aattatctat atcatcagta atgccaggaa ctaaaacatg tcttatccat acaggaatat
     2701 ttcttgattc taagtgtcta gcaagtttta atgccttatc cataccaaca ccagtcaaat
     2761 caatagattt ttcctcaacc atatgtttta aatcaagtaa aaccaaatca gtattatcta
     2821 aaactgggtc tatagtttct atatctacaa atccagatgt atccaagcaa gtgtgtatat
     2881 cattttcttt tgctttttta aataattcac ttaaaaattc tggttgaaga gtggattcac
     2941 caccagaggc agttatacct ccaccagaaa acttcataaa agaagtatac ttaagagcat
     3001 ccgttattat ttcatcaact gtgtattctt ttcctgattt aatatcccat gtatccctgt
     3061 tatgacagta tttgcatcta agaggacacc cctgaaaaaa taatatatat cttattcctg
     3121 gtccatctac agtaccaaac gtttctatag aatgtatctt tcccttaatc atagagttca
     3181 cctcacaata tattttaata accttatcaa tagttataat atttctctaa aaaaggccat
     3241 cttataccag aaataattca aatataagat agccttttat ttttattttt tacacagtag
     3301 taatcttagt taaagtataa aaatttaaag actaagccat tttaccgtgg aatgttctgt
     3361 ttataacatc taattgttgc tctttagtta attttatgaa gttaactgca tatccagata
     3421 ctcttatagt taattgtgga tattcttctg gatgttccat agcatcttct aaagtagctc
     3481 tatcaaatac atttacattt aagtgatgag cattttgagc gaagtatcca tccatcattg
     3541 cagaaagatt gtttattctt tcagtcatat cttttcctaa agcagcaggt actatagaga
     3601 aagtattaga tataccatct tgagcatgtt catatggtaa tttagcaact gaagataatg
     3661 aagctaaagc accattagta tctctaccat gcattgggtt agcaccagga gcaaatggag
     3721 caccagctct tctaccatct ggtgtattac cagttttctt accataaaca acatttgaag
     3781 ttattgtaag tatagattga gttggataag aatttctata agttttattc tttcttattt
     3841 tattcataaa gctttcaact aactctacag ctatactatc aactctatca tcattatttc
     3901 cgtattttgg ataatctcct tctatttcaa aatcaacagc tattccttgt tcatttctaa
     3961 tagttttaac cttagcatgt tttatagcag ataatgagtc tgcacaaact gataatccag
     4021 ccataccaca agccatagtt ctaaatatat ctctatcatg taatgccatt tctaatgctt
     4081 cataagaata tttatcatgc atataatgta ttacatttaa agtatttaca tataatgttg
     4141 ctaaccaatc agtgaatggt tcaaatttac tcataacttc atcataatct aagtactcag
     4201 aagttatagg ttcaaatctt ggtccaactt gaacaccaga tttctcatct acaccaccat
     4261 ttatagcgta taataaagtt ttagctaagt taactctagc tccaaagaac tgcatttgtt
     4321 taccaattct cattgcagat acacaacaag ctataccata gtcatctccc cagtatgctc
     4381 tcattaaatc atcattttca tactgtactg aacttgtatc tatagatact ttagaacaga
     4441 aatctttgaa tccttgaggt aatttagttg accatagaac tgttaagttt ggttctggag
     4501 aaggccctaa tgtatataga gtatttaaca ttctaaatga gtttttagta actaatgttc
     4561 taccatctat tcccatacct cctatacact cagttaccca agttgggtct ccagagaata
     4621 ggttattgta atcaggagtt cttaggaact taaccattct taatttcata acaaaatggt
     4681 ccatatactc ttgtaattct tcttctgtta cgattccagc ttttaaatct ctttcaaagt
     4741 atatatctaa gaaagtagat gttctaccta aagacattgc agcaccattt tggtctttaa
     4801 cagcacctaa ataaccaaag tataaccatt gtacagcttc ttttgagtta gttgctggtt
     4861 ttgagatatc aaatccataa gattcagcca tttttttaag ttcatttaaa gctgatattt
     4921 gctcagttat ttcttctctt aatctgataa cttcctcatc catgcaagaa acttctaatg
     4981 attttttttg ctcattttta tcttctatta aagcatcaac accatataga gcaactcttc
     5041 tgtagtcacc aattattcta cctctaccat aagcatctgg aagacctgtt attataccag
     5101 attttctagc agctctcatt tctggagtat aaacgtcaaa aactccttgg ttgtgtgttt
     5161 ttctgtattt agtaaatata tcttttattt ctgggtctaa ttcatatcca aaagcttcgc
     5221 atgaattttc aaccatcttt ataccaccat atggcatgac agctcttttt aaaggggcat
     5281 ccgtttgtaa cccaactatt gtttctaatt ctttatctaa atatcctgga gcatatgcag
     5341 ctattccaga aacagttttt gtatcaacat ctaaagtacc accattttct ctttcttttt
     5401 taaataggac cattgcttcg tcccataatt ttttagtatt ttcagtggca ccagctaaga
     5461 aagagtcatt tccctcatat ggagaatagt ttaattgtat aaactctcta acatttattt
     5521 ctttagtcca tcttcctgtt ttaaatcctt gccatgcatt cattaagtat ttcctcctta
     5581 gatttaaatg ataaataata ctattaaaat taatttttat atacttaatt tattttatat
     5641 atttttttaa aaatatattt tctaatataa taatagcata ttattcgtat cctgtataca
     5701 taaaaaataa aaaattgtcg ctttaaagta taaaacagat tatatgttaa taatgtggtg
     5761 aaattatacc catatataaa taaaataaaa caaataaata gctaattaag gagaaaatat
     5821 tttttatttt ttgtttagga taaataaatt ttattatgga aaaaagaaaa agcatacatc
     5881 ttcacaaact gaatatttat ttaatagggg gaggtgatag attgtttatc acagtggtat
     5941 tatttttagt aggtttccta ttaattacta aaggcgcaga tatctttata aattgtactg
     6001 tagagatagg taaaaaaacg aatatatcag aaattatact tggagctact atagttagtt
     6061 ttgcaacaac attacctgaa tttacagtgt ctctacttgc ttctattgat ggtcatacta
     6121 ctatgagttt aggaaatgca gttggttcta taatatgtaa tacagggtta gcattagggt
     6181 tggttgtttt tataagtcca tttaatgtag ataaaaaaat gtttttttct aagtctttac
     6241 tattaatagt atccgttata gttttaattt tattgagttt agatggagtt ataacaagag
     6301 gtgattcatt attacttata ataatattaa tcttttatat ggttaataat tatagaagtg
     6361 tcgttggaaa atctgatact aaaaatagaa atattaaaaa taacactatt aaagacatat
     6421 ctactaaaaa agggaaaaat tataggggtt tttccgtatt agaaattgta aaaatactat
     6481 tactttttgc tacaggattg ataatgatga taataggttc acaaatcctt atagagagtg
     6541 gagtaataat agctagtttt ttaaatatac ctcagggtat agtaagttta actataatag
     6601 cacttggaac ttctcttcca gagatagttt cgtcgattac tgctataaga aaaaatcatc
     6661 atgaaatatc agtaggaaat attttgggtg caaacatatt gaatatagta tcagtaatag
     6721 ctgtatcagc aataccaaat aacataccaa tactatctca aaatagacag ttggatatac
     6781 cattcatgat attattatta ttaatagtaa taataccaac acttaaatct aataaactta
     6841 gtagaataca aggtatattg atgttattca catattttct gtacatatca attttgtatt
     6901 tcatgtatat tatttaacta tactgtttaa aatattaatt gcagaatatc ctaaaaaagt
     6961 atataatgga taggttaaga ttaattgata acggagagga ataatatatg aatataacaa
     7021 agtttgaaga tttaccaata agtgagggaa taaaaaaagc tatagctgaa atggggtttg
     7081 aagaaccatc tcctatacaa gcacagtcta tacctgcgat tttgtcaggt aaagacgtta
     7141 taggacaagc tcaaactggt acgggaaaaa cagctgcttt tagtatccct atactagaaa
     7201 caatagaccc aaataataga agtttacaag ctgtagtgct ttgtccaaca agagaactgg
     7261 caatacaagt atcaacagaa ataagaaagt tagctaagta ttcgcacgga ataaagacat
     7321 tacctatata tggtggtcag ccaatagata gacaaataaa atctctaaag agtggtgtgc
     7381 aagttgtaat aggtacacca ggacgtacaa tagaccatat caatcgtaaa acattaaaga
     7441 tggataatgt aaaaatgatt atattagatg aagcagatga aatgctagat atgggattta
     7501 gagaagatat agaaatgatt ttaagtaaga ttccagaaga aagacaaaca actttctttt
     7561 cagctactat gccaagagga attttggaat taactaagag atatcaaaaa gacccagagc
     7621 atataaaagt tgttagaaaa gaacttacag tttcaaatac aaagcaatac tatatagaaa
     7681 ctaggtcatc aaataaatta gaagtattat gtagattagt agatgtatat gaccctaaac
     7741 tatctgttgt tttctgtaat actaagagaa aagcagatga attggtagga gatttacaag
     7801 caagaggata ttttgcagat gcattacatg gtgacttaaa acaaactcaa agagatatag
     7861 taatggataa gtttagaaat ggtacaatag atatacttgt tgctactgat gttgcagcta
     7921 gaggtataga tgttgatgat gttgaatgtg tattcaacta tgacttacca caagatgaag
     7981 agtattacgt tcatagaatt ggtagaactg gtcgtgctgg tagagaaggt atgtcattta
     8041 cttttgtatt tggaaaagaa atgagaaaga tgaaagatat agaaagatat actaaatcta
     8101 aactaataaa gcataatata ccaacaataa ctgatgttga agagaaaaaa gtaggtactt
     8161 tctttgcaca agttaaacaa actatagaag aaggtcactt aactaaacaa ttacaatggt
     8221 tagaaggttt ctgtaatgat gaagattatg caatggttga tatagctgct gcacttgtaa
     8281 aactatctct aggtgaagaa atgaaagaag aaataataga agaaaaacct agaagagagc
     8341 gtggagatag aaaaggtggt actggagcta aagatggtat gattagatta tttatcaata
     8401 ttggtagaaa tcaaagagta caagcaaaag atatagttgg tgccatagct ggagaagttg
     8461 gaataccagg taaagtagta ggtactatag atatatatga taagtatact tttgttgaaa
     8521 tacctaaaaa agatgcaaaa acagtaattg aaaagatgaa agatattaag ataaaaggta
     8581 ataagattaa tatagaaaaa gcaaataaga agaaaaaata acgtatagta tacaaaacta
     8641 taattagaaa atatataaaa taatatattg gatatttaaa aagcctgatg tgttttcaaa
     8701 acagatagta gataatctgt aagagttaac agcataaggc ttttcttgtt ttatatgatg
     8761 ttataatttt ccagattatt gtgaaaaatg tatattagtt cctttttttc aaatctgatt
     8821 tgaaaaagag aatattaatt atctatatag tttagattat aataaaaaat gtaagataac
     8881 aaaggggaga aaaagatgtc aatgacttat atggacccta gggttttttt gattttagaa
     8941 atggtgttag aagaagacac aaatactcta gaacaatttt caaatgtttt gggtgttagt
     9001 actaggacta tcagaaacta tataaaacag attaatcgag aattaaaagg tggtattgct
     9061 aaaatagtta aaaatgaaaa aggtacatat agtataagaa ttgaggatga aagtaaatta
     9121 attgagattg taaattttaa tagaggaaag ctatcaaatt ttataaattt aaattcacct
     9181 gatgaaagaa taaattatgt tcttgatatt ttaactatga ccaacaatcc aattaccata
     9241 gatgatttgg ctgaagagat atgtatagga agaacaacat taataaagga tttaaaaaaa
     9301 gcagacagaa ttttagctga atatgattta gaattaaaaa ggaagccaaa tataggtatg
     9361 attttagttg gtgaagaaat ggatataaga ttattaatcc ttgatagatt gtatgaaaat
     9421 tatattgatg tcttggaaaa cgttaatcag ataaatttag ttaaagatta tgatattgaa
     9481 tgtcttagag atgaattaaa aaagttattt aaaaaagaag aactctacat aacagaacag
     9541 gtcttgagag atgttgaaag atatataatc atgtctgtct taagaaatct aaatgggtac
     9601 aaatttgaaa agatagataa gagatttgaa gtgataagat gttcagatga atatacttta
     9661 ggattaaagc tcaaagcttt attggaaaag atatttaata tagttttaaa tgaaaaagaa
     9721 accatatttt tgacaatgcc attaataggt agaaaagctc ctagtacaaa attagcactt
     9781 agcagtataa agataaatga tagcgtaaag gaagttgtag atgaagtaac tagtttttta
     9841 ttagagacct cagggattga ttttaaagaa gataaaaagc ttatagaaaa tttggaatat
     9901 cacttgtatt ttgcacttaa tagaatgaga ttcaacataa gggtaaaaaa tcctctttta
     9961 caagaaataa aggaaagata tacatttcca tatagtgttg caaagatagc tgcaatgata
    10021 attgaggaaa agtttgactt gaaaatatgt gaccacgaaa taggatatat agcattacat
    10081 tttggaagtt attttgaaaa aaattcaaga aaagttctaa atgtaaatag ggtagctgtt
    10141 gtttgtggaa caggtcttgg gactgcacaa cttattagaa taaaggtagg aaaaatactt
    10201 ggagaaagtg tagaagtaaa tactttttct gatatagaag ctaaagctaa tttagattta
    10261 ttaaagtatt atgatttagt atttacaaca gtagatttca aaacagactt agatgttcca
    10321 atttttagag taaatgcgtt atttaatgaa gaaagtataa agaaagagat agaaaatgca
    10381 atacttttga aaaatagtat aaatcttaag tctataaaaa agattgaaaa aaatatccct
    10441 tttataggta aattaattga agaggataag ttctttatat tgaataagag tacgtttatg
    10501 gaaaatttag aagatatgtt agaaaaatta gttcataaag gaattattga tatagagttc
    10561 aaaaaaaagg tattaaaaag agaaaagaaa tcttcaacag cattagataa ttatatagca
    10621 ttgcctcact cagttaatac taatggggaa aatctttatc tggcatttgg aatattagaa
    10681 aaaccagtga tttgggatac aaaggaaatt agaataataa tattgatgat aattccagat
    10741 aagaatgtag atagtacaga cctgataata aaaagttatg aagaagtact tgaagtaggg
    10801 agagataaaa agatggtaga agaacttgct aaggtaaaat catttgatga attttataaa
    10861 ataattacca agaggaggta ataatatgga cccagtaatt tttattatag catcacttat
    10921 tacagtgtat ttattaaata tttttcttgg atacttacag cttaaagatt ttaataaaaa
    10981 ttatattgaa ctaaaaagaa aaggaagagt ggcaattggc agaaaaaaag gaagaatttc
    11041 atcgggtaca attgtgttga tacttataaa tgatgatggt gtgattgtcg agactagaaa
    11101 aatgcaaggt gttacagtat tggcaagagt aaagtcattt gaaggtttgg taggaaagag
    11161 cttagagtat atagaaaaat cagatttaac tgtttacaat aaactactta aaaaagcaat
    11221 actagatgca gtcaaacagt acataacatt tgagaaaaat aaatgtgaag atacaaataa
    11281 tctgagtact gatgaagagt gtaggatgga agtagtttaa aaaaatatat ttataaggag
    11341 gagttaatat gacgcaattt tttcaagtat tagcaagtgg tgcagatagt tttatgaatt
    11401 tatttagggc aggtgggcaa caatttgtag gttttgtaac agatatagta ccacttttag
    11461 tagcattatt agttacaatg aatgcaatta tcaactttat tggtacagat agagtcgaga
    11521 gacttgcaaa aaaatgttct gcaaatatat ttactagata tttgatatta ccagtccttg
    11581 gtacatttgt atttgcaaac ccaatgacac tatcattagg aagattcttg ccagaaaagt
    11641 ttaaacctag ttactttgca gcagcaagct tttcatgcca tacaatgaat ggattattcc
    11701 cacatatcaa tccaggtgaa ctatttatat atctaggaat tgcaaatggt ataacaactt
    11761 tgggattctc aacagcagat ttagcagtta gatatttgtt agtgggtata gttgcaaact
    11821 ttattaaagg tgtagttact gactatacaa caaaatttgt agagaaacaa caaggaatag
    11881 cattaaattc agatataaag attggataat tactttaagg tataaataaa tttttcaaat
    11941 aaaaagatag tgtatagagg aggataaatt atatggcatt taacaaagtt aaaatagtaa
    12001 aaggttctgg aggatggggt ggaccattga taatagaacc aacagaaaag aagaataagg
    12061 tagtatatgt gactggggga gcaaagccag aaactgcagt aaaaatagct gaacttacag
    12121 gatgtgagtt aatagatgga ttttcacagg gtgttaaaga tgatgaaatc gcatgtgtaa
    12181 taataaattg tggtggaaca ttgagatgtg gtatatatcc tcaaaagaaa ataccgacta
    12241 taaatataat gaaaacagga aaaagtggac cattagcaat gtttataaaa gaagatatat
    12301 atgtttcagg ggtaaaacca acaaatgtag agcagttata attgaggtag taggagggaa
    12361 gaaatatggc ttatgatact aaaaagaaga taagtgagca gagtcaagga attgttgcaa
    12421 aaataggtat agcactagga aatgtgacaa gtactctttt tcaagctggt agggatacta
    12481 tagatacatt aatacatact atactgcctt ttatggcttt cgttgcagtc ctaataggta
    12541 taataaatca atcgggtata ggaaattggg tagccaattt acttactcca ttaatcggaa
    12601 atgttggagg tttagttgtc ttatcaataa tatgttcatt tccgttgttg tctccgtttc
    12661 ttggaccagg agctgttata gctcagatag tgggggtatt ggttggtaca gaaataggaa
    12721 aaggaaatat tccaccaagt ttagcattac cagcattgtt tgcaataaac tcacaagcag
    12781 cttgtgactt tataccagta ggtcttggtc ttgcagaagc tgaacctgat acagttcaag
    12841 taggagttcc gtcagtttta tattctagat ttttaacagg tgcaccaact gttctattag
    12901 catggttagc aagctttggg ttatataaat agaagaatac tttataaata attagagaga
    12961 ggttcagttg atatggaatt aatatacagt actcaaataa ataaaatagg agataatgca
    13021 aatgaatttt tacaacataa tatgtttgta actttcaaag ataatgctcc tcaagagtta
    13081 gaaaattact gttatataca ttctgaaaat aatttagtaa aagaaatact tacggatgat
    13141 gtgctatcaa taaataatgt tgattacaaa attacttctg ttggtgagtt agtaaaccaa
    13201 aatctttcag aattaggtca tataacattt aagtttacag gtgaaaaaga tggagcaatt
    13261 ggaggaacct tatatcttga agaaaaagaa atagcaccta ttggcgtagg tactatacta
    13321 aaaataatta gaaaataaaa tttaaaacga atctaatcat attataagta aattatagat
    13381 aaaagtcaat ttaactaatc aagttaactt ttaatgtgat aaaaaagggg aaatgaaaat
    13441 gagaaaagtg gctttagtcg ttggaggagg aagaagttta ggtgaatatt tatctaaaca
    13501 tttagctaaa gtaggttatg atgttgctat tgcagattta aatgaagaca acgcaaaaaa
    13561 agtagctgaa gaaataaaaa ctgaatttgg ttgtaaatct gtaggatata gttgtaatgc
    13621 catagatgaa agacaggtta taagtactgt caaagctatt gaaaatgatt ttgatagaat
    13681 agatttatta gtgtacaatg caggagtggc tgtaagtaaa aaaataactg attttggata
    13741 caaagatttt aaatttattg tagacgtaaa tttaaatggg tactttctat ttgcaagaga
    13801 agtatctaaa gtcatgatag caaaaggtat tgagggtaga ataatacaga taaattcaaa
    13861 atcaggtaga gtgggaagca agcataattc aggatattca gcaggtaaat ttgctggagt
    13921 aggacttact caaagtttag cgctagattt ggcagaacat aagataactg taaatgcact
    13981 gatgctcggt aatttacttg attctgatat gtttgaaagt ttaattcctc aatatgcagt
    14041 aaaattggga atacctgaat cagaagtcaa gcaagtttat atagataaag tacctttaaa
    14101 aagagggtgt agatttatag atgttgcaaa tatgcttaca ttttatgctt caaaggaagc
    14161 tgaatactgc actggtcaat ctataaatat tactggtgga caagttatgt aaggttttta
    14221 tggagtgtct caagatgaac tctttagttc atgaggcact tttttgtata aaattaaata
    14281 tattttcagc atttaaacaa tgaaaaagag gtttatcttt attttgagat agcctcattt
    14341 ttgtaattaa aaaacatagt gtttaagtaa acatataaga ttgttagttt tttaaaagag
    14401 aattttttaa taatattata tttatgaagt atttttaaac ttaatttgat aaaatatagc
    14461 tatgttttat atactgtagt tttgaacata gaaagtttaa taaattatag tatatatgtt
    14521 aatttttaat aaggtaagaa agtagaaaga aggatatttt atggatataa taaaaaaatt
    14581 aagtcatggg tgtacattag attgtcatga ctgttgtaaa ttcaatgttt atactaaagg
    14641 aaataatgta gtaaaaattg aaggagataa aaatcatcct tatacaaaag gttttatatg
    14701 caaaaaaggg atggcccatt tagatagatt aaatcataaa gatagaataa agactcctat
    14761 gcttaaagtt gatggagtat ggaaagagat ttcttttgat aaagctatag aaataatggc
    14821 agaaaagctt acatactata aagaaaagta tacatcaaag tctgtcatgc attatgacca
    14881 atatggaagt gggtctgtgc taaaatacat aggagatata ttttttaatt tttatggagg
    14941 tgttagcaga cataaggggg gtccctgttg gagtgcaggg atgcatgctc aaaaatatga
    15001 ttttggagta gctaagagtc atgctataga agatatgtta aatagtaaaa gtatatttgt
    15061 ttggggaaaa aatccagcat atactacaat tcatactatg caaataatta aaaaagctaa
    15121 agaaaaaggg ataaagatag tagtgattga ccccatatat acaaaaacag ctcaaatagc
    15181 agataagtat gtacaagtaa atcctggaac agatggagct ttagctatag ctatggcaaa
    15241 aattatagta gaagataaat tatatgatga agaatatata aactcgtatg ttattggttt
    15301 tgaagaatat aaaaaatatt tatcttcatt ggaattaagt tttttaattg atgaatgtgg
    15361 tgttaaagag aatgatataa gagagttagt ggatttatat actaataaat attcaagtat
    15421 aaatgttgga tatgggcttc aaaaatataa aaatggtgga aacacaataa gagcaataga
    15481 tgctttaggt gctataactg gtcaaatagg atttagtggt ggaggggtaa actatgctaa
    15541 taaagtttat ccgagtgtta taaattctga cccttacaat agccaatcat atggtgaaga
    15601 tagggagttt tatgtaagta atatcagtaa atttatagaa aaatctttaa aaaatacatc
    15661 aaataaagta aattatgcat cagatgaact tgatatggca ttacataaag taaattatgt
    15721 gtcagatgaa cttgatacaa catcaaataa agtaaattat gtatcagatg aacttgatat
    15781 ggcattacat aaagtaaatt gtgtatcaga tgaacttgat atgacatcaa ataaagtaaa
    15841 ttgtgtatca gatgaacttg atatgacatc aaataaagta aattatgtat cagatgaact
    15901 tgatatgaca tcaaataaag taaattatgt atcagatgaa cttgatataa catcagataa
    15961 aacagattat atatcaaatg aattgtataa tttatctaat aagtcaatca cagataatat
    16021 tcctataaag atggctgtga taactaaaag taatatgctg aatcaactgc cagatttagt
    16081 agaattggag agggtgtttt caaaaattga atttaaagta tgttttgata tgtttataac
    16141 tgatacagct actttatgtg acatgtttat accgtgtaca aatacactgg agagtgaaga
    16201 tataatttat agctcaatga ccaatccata tataacctat aatgaaagag ctgtaaagcc
    16261 agcacataaa ttgatggatg aatattactt ctttatggag ctagctaaaa agatgggatt
    16321 aaatgattat ccttttgtag aaaagagaac ttatttagaa aaagtgattg aaccacttaa
    16381 gcgttttgac aaaaatctgg atatagaaaa acttaaaaac aattacttta ctatacataa
    16441 ccctgttgct tgggaagata agaagtttga gactccatct ggaaaatacg agctttattc
    16501 agaaagtata aaaaacttgg gaataagtcc aacacctgta tatataagca ataaatataa
    16561 aggaatagaa gataaaaata tttcttttag attattaact aatcatcatg cagatacttt
    16621 atttagtcag cattttatgg acaaaaaatc tatagctcaa gcatatataa atcggagaat
    16681 ggctaaaaaa gtaggtattg aggacaaaga tattgtgata ttgaggtcaa aaaaagcaaa
    16741 aataaatgta caaataaata tagatgatgg agtaggtaat tatatagtaa aaatgtatgt
    16801 tggttggtgg aagaaacatg gaaatcctaa tagtttaaca gacactggta tatcagattt
    16861 tgggggtcaa gtaacatata atgaaagtat ggtagaaata atacgacaaa actagttaaa
    16921 tttcattgaa ggaataaaaa tataattata gaattgatta aaagttacta tttttacaaa
    16981 actattgaag gagggatttt tgatggtaaa ttattcctta gaacataaga atctatacat
    17041 agaatttatg tgctcagaac tagaccacca tgtagccaat gaaataaggg aagaaataga
    17101 taacttatta agtgtaaatc aagtaaaaaa tgttgtattt aattttgaaa atattaattt
    17161 tatggattct tcaggtatag gcgtcatcat aggtagatat aaaaagattt ctaatgaagg
    17221 gggacgagtt tcagtaatta atataagttc acgagttaag aagatttttg atttatctgg
    17281 gttaaataaa attattggca tttatgatac gtatgaagaa gctctaagtt ctttgtaggg
    17341 aggagtatgt gatgaataat attatggaag ttaaattttc ggcaatatct gaaaatgaaa
    17401 gtttagcaag agttatagta gcatcctttg cagctaagtt agacccaaca gtagatgagt
    17461 tagtagatat aaaaactgct gtctctgagg ctgttacgaa tgctataata catggatatg
    17521 aagaagacag ttctaagttt gttttcttaa gatgtgagat agaaggaaat actataaaga
    17581 ttatagttga agatgaaggg tatggaatag aaaatgtgga gaaggcaatg gaaccacttt
    17641 atacatcaaa accagaactt gatagatcag gaatgggatt cacagtaatg aaaagcttta
    17701 tggacgatgt agaggtaagt tctgtaaaag ataatgggac tagaattgaa atgactaaaa
    17761 aaataaatgt gcctaaataa agtccaaaag tgaggtttta ttatggaagt aactgttgcc
    17821 agagaagaaa aaaaacctct tcttagtcat gaagagactc tagaacttat tgaaaaagtt
    17881 caaaatggag atgaagaggc aaaagaaata ttaatatcaa gcaatttagg tctagttagg
    17941 agcgttgtat ctagattttt aaatatagga tatgatagag aagatttatt tcagttaggt
    18001 tctataggct taataaaatc aatatataaa tttgacccaa aatttaatgt aaaattctct
    18061 acttatgcag taccaatgat tttaggtgaa ataaagagat acttaagaga tgatggtatg
    18121 ataaaagtat caaggtcgtt aaagcaaata gctgtaaagg caaagatgga aagtgaagct
    18181 ttaactaaaa aattaggtag agagccgagt atagaagaat tagctaaagc aatagatgta
    18241 gaaaaagaag atttagtaat ggctatggaa gctaatttca atgttgagta tttgcaagga
    18301 gttattcatg aagaggaagg ttctccaata tgccttattg ataaaataag catgaaagga
    18361 gaaagtgaag aagaaaaagt agtggataat atattgttaa aagacatact tggaagactg
    18421 gataagagag agcgtcaaat aatagttctc agatactttg aagacatgac tcaaagtgaa
    18481 ataggagaga tgctcaatat atcacaagtt caagtatcta ggatagagaa gaaagtattg
    18541 tctaagttaa aagaatatat atcatgataa tacttatgat atatagaata acaaaatata
    18601 ataaatatat tactaaaata aaatgtaata actttttatt ttaagttttt aaattattaa
    18661 aagatattaa aataataaaa agcattaaaa aagtaaaaat ataaaaacaa ggtgtcttta
    18721 tggaaaaata gattttttct ttattaggca tcttttttat tttttgacca aactttagat
    18781 agtcaaaata aataataggt ttattttttt aaaattaaca aataatactt tagagaggtg
    18841 tttagtatgg ataaaaatta taaaaaatat gtagaccaaa taagcccaaa acctacatat
    18901 ttaaaaaact atacacttgc atttattgta ggtggaatta tttgtatgat agggcaagca
    18961 ataaatgatt tgtatatgaa agttggaggt cttgataaac taggtgctag ttctgcaaca
    19021 tcaataacat taatatttat aggggcattt ttaactggat taggagttta tgacctaata
    19081 ggaaaaagag ctggagctgg ttctataatt ccaataacag gttttgctaa ctcaatagta
    19141 tctccagcaa tggagtataa aagagaaggc tatgtattag gtgtaggtgc aaacttattt
    19201 aaaattgcag gaccagtttt agtttatggt ataggttctt ctattttatg tggaattatt
    19261 tattatattt ttaagatgtt ttagatgatt tttatttaat ttaaatttta ttaattatga
    19321 agaaggtata aaatctgaaa atagctacta aagtaactaa ttgaaaaata attgtgcaga
    19381 tttaattaat atttttaatt aatattaaaa atttgaatag taaaatgcaa aattaaaaag
    19441 gtgtgaaaat atgaaaaata aaagaattgg aaaaagaaca gtcaagttag aaaataaacc
    19501 aaccataata tcaactggaa ctatagtagg tcctaaggaa ggtgaaggac cactaaagga
    19561 ctattttgat atgattatga ctgacgattt atatggtgaa aagacttggg aattagctga
    19621 aagtaaaatg gtagagactg catctcaaca agctatccaa aaggcaggta aaaaattgtc
    19681 agatgtaaat tatatgctag gtggcgattt aataaatcaa atagttccag catcatttgc
    19741 agcaagagaa ctggctattc catttttagg tatatatgga gcttgttcta caatggcaga
    19801 aggtctttgt ataggttcta tgcttataga tggtggtttt gctgatttag tactgtcagg
    19861 tacatcaagt cactattgta cagcagaaag acaatttaga ttcccacttg aacttggaaa
    19921 tcaaaagcca atgacagctc agtggacagt tacaggagca ggaagtgtat tattagctcc
    19981 taatggagac ggtccaaaag taaagtatgt tactgttggt aaagtaatag atgagggtat
    20041 tgatgatggt aataatatgg gaccagctat ggctccagca gcaatagata ctatctactc
    20101 ttactttgaa gatactaatg atgacccaaa tagctttgat ataattgcta ctggagattt
    20161 aggtactctt ggaaaacaga tagcagaaga ctttttaaaa gaaaagggtg tagatatatc
    20221 aaaagtttgc actgactgtg gtatagagat gtttaacctt aaagaacaag atgtccattg
    20281 tggtggaagt ggatgtggat gttcagctac agtgtttgca ggatatatat atgataagtt
    20341 gagaaaaaaa gaatttaaca aagtaatgct tgtatcaaca ggagcactac tttcaccgac
    20401 aagtacacta caaaagcaga caataccatg tgttgcacat gcagtagtaa tagtaaatga
    20461 gtaggaagtg atataatatg ttaatggact atgtaagagt atttattgta ggtggaatta
    20521 tatgtttaat agcccaagta atgatggatt atttcaaaat gcaaactcca tatgttatgg
    20581 taacgtatgt aacaactggt gtagttttaa cttttctagg actttatgag ccattagtta
    20641 agtttgcagc agcaggtgca acagttccta taataggatt tggatattca cttgctacag
    20701 gagtaataaa atcaattcaa agtgatggtt ttttaggtat ttttttagga ggtacaactg
    20761 ctacagctgg tggaatagca gcagctatat tctttggata tattatgtca gtagtattta
    20821 ctccaaaagc aaaaccataa atctttatta aaagaataat aagtttttga aataataact
    20881 ttttataaag caggtggata tcttttagat tccacctatt ttttatataa agaagcgttt
    20941 acataattga agttgacaat tatttacaac atagtgtagt attatattat caaaataaaa
    21001 tgaagtgaag taggggataa agatggtaac tattaaagat attgcaagga atctaggtat
    21061 cagttattca actgtttcta ggtgcttaaa caataatcct aatgtatctg aaaaaacaaa
    21121 aaagaaagtg gtagaagagg caaatagact agggttccat tttaatgtaa atgcaagaaa
    21181 tttagcaaaa aaagaaacta acaggatagg ggtaatcttt tcaaataatt ttaatcatca
    21241 agatactaga aagtttttta gtgatataat ggatagttct ataaattcta ttgagactaa
    21301 taaatacgat tttataatac agcctaataa taacattagt ggtgatagta atgtatataa
    21361 aatggtaaat ggacaaatgg ttgacggatt agtaattgtg tctcaatcaa taaaaaaaga
    21421 agagtatgat tttttaaaag acaataattt tccacatgta tttatatatt ttaaaccatc
    21481 ttttatagga gaagtagata atttcttttg ggacgataac gtgtatggag gttatatagc
    21541 taccaagcat ttaattgacc atggtcataa agatataata acagtaactt cagatgataa
    21601 gagttcaaaa atgcatgaag atagaaccaa gggatatttg aatgctatgg aagaagcaaa
    21661 tttaaagaca gaagtcatta aatctaaaat ggattttgaa tctcaagttg aatttttaga
    21721 aaaatacatt gataaaataa aaaaagcttc agctatattt gtacaacaag atgtaccagc
    21781 gatttctata atacaagagt taaaaactac ttatggtata aatgtaccag aagatatttc
    21841 cataataggg tataataata ttgaattaat atcatatttt aggtcgcatt tatcaacaat
    21901 agatgaccca agagagcaag ttataaaaaa tggagtagat agtttagtaa atataataaa
    21961 taaaaagagt acagaacaca ttccaagaaa attatatcca aggttaataa tacgaaatag
    22021 cgttaaaaga ataaagtagt aagcttttaa gtatcaaacg tttattaaaa atattataga
    22081 ttatgtgaag ttttttaaca aataatataa ataaaaatta aaaggagtag gtgtatataa
    22141 gtacattgta ctcctttatt tattgagata gtttttataa tatgaatgaa taattttatt
    22201 caattatttg aaaatccgtt attttaacaa tataaattaa taaattattt gtattttata
    22261 tcatgaatct ataatatttt tttgattcat aactaaaagt atataaaaat gatgaataaa
    22321 tatatattat acattatcaa tgataggtta tataataaaa tcactaacaa ttatattata
    22381 agaaaattat aaaaattaca aggaggtata gaaatgaaag aaaaagcagg gattagaaat
    22441 gccataatag cagcctttat aatcacaata ttggcagaat taattggacc tatttcattt
    22501 aaagttatgg gaattaatgt aactttactt agtatactat gggctatatt cataggaatg
    22561 gctgtttctc ctcatttatt agggagagtt ataccagcct taaaaaagtt tataggggat
    22621 aatgaaataa atgtatctcc atatttatta tctttgacac tatatccatt agggataatg
    22681 tttggaatta atgcagggcc taaagttggt atattacttc aagcaggacc agcactttta
    22741 ttacaagaat ttggtcatat gggtacaatg ttgatagcat tacctgttgg agtagcattg
    22801 ggtcttggaa gaagtgcatt aggtggtact ttttcattat gtcgtgacac agcactagga
    22861 attattggag ataagtatgg attaaactca ccagaaggta taggaacact aggaacttac
    22921 ataagtggta gtatatttgg aacattatta ttttcatttt tagcaccagt gggtgtactt
    22981 ttaggatttc atccatatgc tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg
    23041 ggagctgcaa cagcatcatt aatgaatacg gttccacaaa tgtcagagca gattttagca
    23101 tattcagcta ctagtggatt gattacagct gttactggaa tatatataga attattctta
    23161 tctttaccag tagccaacta ctattatagc aaagtagctc cagttattga aagatttaga
    23221 ggaagaaaaa caagaagaac aagtaataaa ttgtaaagga ggatatatat atggaaaaaa
    23281 cttttaagac tattatggag cagataaaaa tagtattgtt tatatatatt attatttgct
    23341 ttggacaaat gatagcactt aagactcagg ctcaagattt gttgattgga agtgtatctg
    23401 gatttttgtt ggtgataatt gctattataa taaagaattt tgttaaaaaa cctaatttac
    23461 caggttttgc atgggctaca ttagttgcat tcgttttatc gcttccaata tctccagtgg
    23521 gggatatcat agcaacaaat gtagggaaaa taaattttat gtctacagta acaccattat
    23581 tagcttttgc aggaatctct gtaggtaacc aattagatat attaaagact ttgagttgga
    23641 agttagtagt tatatcattt atagttatga catctactta ctttggttct gcttttatat
    23701 ctcaaacagt tttaagatta aatggaatgc tttaaggagg tagtaaatat gaatgaatta
    23761 aaagcaaaaa ttaaagagga aataaagtta ttatctgaag agaaaaagac atcatttgaa
    23821 aaagtttctg actatttatt ttcaaatcca gaattagcat ttgaggagta taagtcacaa
    23881 aaagcattat gtgatttgtt agaagaaaat gaatttaatg taactaaagg tgtaggagga
    23941 ttagaaacat cttttgaagc tgtgtattca aatggaacta atgggaaaac agttgctttt
    24001 ttagctgaat atgatgcact tccaggaatg ggacatgcat gtgggcacaa tatcataggg
    24061 acaagttctg ttggtgcagg tatcatatta aaagaaatca tgaaaaaaca taatatagaa
    24121 gggactgtaa aggtttttgg aacacctgca gaagaaagag taggtggaaa aataactatg
    24181 attaaagaag gtgtatttaa taatgtagat gctgcattaa ttttacatcc aagtgatgct
    24241 tcaatgcctg atgatatatc atttgctcaa gttaatttaa aatttgattt tactggtaag
    24301 gcatcacatg ctgctgcttt tccttgggaa gggaaaagtg ccttaagtgg agtaatagca
    24361 ctatttaaca gtgtaaatag catgagatta catcttaaag attatgcaag agtacatgga
    24421 attataactg atggaggtag catacataat ataattcccg aaaaatctac agctatattt
    24481 aatgtaagag ctttaagcat agaatattta aatgagattt gtgaaatgct taaaaattgt
    24541 gctaaaggag ctgcaatttc tactggaact aatgtagaaa tagttcaatt agatgaaata
    24601 tacaaagaga ttaagaatga ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt
    24661 ttgggtgaag actatgttga aagagattta agtcaaggta taggttcaac tgatacagga
    24721 aatttaactc atgagatacc tgcaatacaa gcatatataa aattaaaaga aaatactgca
    24781 actcatacag atgaatttgc tgttgcagct ggtggtgaag aaggcaaagt agcacttata
    24841 aaagcaatta aggtgctagc aatgtgtgga gtagatattt tgtatagtaa ataatatata
    24901 attttaaaat atattaagaa aggaaatcag attagacatg atattaaaac aagtaataga
    24961 ggtttatgac attttagata aagcaaatgc taatggggaa gaagtaaaaa cttacttaca
    25021 aggttatggt gaagtagatg taactgtaaa agaattgtct tcaagtaaag gctcaacaga
    25081 tttagtaaag cttacaattc ctgggaaaaa tggaaaatta aaaggtggag atgctcctac
    25141 tcttggcatt ttaggtagac taggagggat tggagcaaga ccagaagtta taggttttgt
    25201 ttcagatggt gatggagcat tggtagcaat tgcagtggct gctaaacttt tggatatgca
    25261 acgaaaaggg gatatattag atggtgatgt tgtaatttca acccatattt gcccagatgc
    25321 tccaactaaa gaacactatc ctactccttt tatggattct ccaattgata tgatgactat
    25381 gaatgaaaat gaagttgata gtagttgtga tgcaatactt tctatagata ctactaaagg
    25441 aaatagagta atcaatacta gaggatttgc aatatcgcca actgttaaag aaggctacat
    25501 attaaaaact agtgataacc ttttagatat tatgcagact gtaacaggga aatctccatt
    25561 tgtattccct ttgtctattc aagatataac accatatgga aataatctgt atcacttaaa
    25621 tagtatttta caaccagcag tagctacaga tgcaccaata gtaggagtag caattacaac
    25681 agagatgcct gttgctggat gtgctacagg tgcaactaat tttagtgatt tagattcagc
    25741 tgggagattt gcaattgaag ttgccaagct atttggtaga aataagtgtg atttctatga
    25801 taaagaagag tgggaaatgc ttattaagag atatggcaaa ttaaatagat ttcaaacatt
    25861 tggtatacag taattagaaa aatattaagt ttatataatt tgtgatacaa ctatttaaga
    25921 agatataaac aattaggaat aaaagtaaga ataaagcact tagttaaaaa ctttaaaaat
    25981 aaaatattta tttgtttata taaaaaagga ggattctggt tattgggatt ctctttttta
    26041 tgaatacaaa taatatattg taattataaa aaaaggagcg tttcaaagtg atttttttaa
    26101 tcaccttgag acaactcctt atatttttat ttaaattcta ttttttatta agttgctgga
    26161 gttccaccac catcaggagg agtagtagag ccaccaccat tatttccacc accatcagga
    26221 ggagtagtag agccaccacc attgtttccg ccattatcag gaggagtagt agagccacca
    26281 ccattatttc cgccattatc aggaggagta gtagagccac caccattgtt tccgccatta
    26341 tcaggaggtg tagaaccgcc accattatca cctgtgttat tattgttatt attattttct
    26401 tctggttggt tattgtctgg tttttttgta tcttcagatg atggttgaga acttacttta
    26461 gtaggagcag taccttcaat aaatgctgct ttagagccag aagattgctt tcctccatcc
    26521 tctaaattaa tttttgtaaa atatacattt ttaggaactt caaattcagt aaccgttaaa
    26581 ttagcatgta ttttttccat aattttttcc catagttttg cagtactagt acttccatgt
    26641 ggtacttctc tacgttttat agtgttacca gaatcatctt ttctaccagc atcatctcca
    26701 atataagttg caccaacata ataaggagtg taaccaacaa accaagcatc atatgcacta
    26761 tttgtagtac ctgttttacc tgctacaggc attccctttg gaagtgcagc aacaccacca
    26821 gtaccttcag taattacact ttctaacata tctgtaacga cataagctac ttcaggttct
    26881 acaactttat gttcctcagg agcattttta actaataatt gaccatcaaa ggtagttata
    26941 gtagtaaaaa tagaaggttc tacataaata ccgtgatttg ccaatgtacc ataagcagca
    27001 gccatttgaa gtggagtaat accattcgcc ataccaccta gtgtcaatga tgggaatttt
    27061 ctatcacttg cttcagatga acctgagtga ttattcttta aagttgttat accaaagttc
    27121 tttaagtaat ccatcattat atctacacat tcatcataag agtctccaag catttcagca
    27181 gttttgacag ctatcgtgtt tgaagattta gcaagtgctt tacgtaaact catagaacca
    27241 tgaccagcag tagtagttct tggattccat ttattatttt cttcaaactt atatcctcct
    27301 cgagaatcac ttagagcagt tgcttgtgtt atttttagag tatcaatagc tggcgtatat
    27361 attgctaaag gttttataga agaacctggc tgatgagggt tagtagcacg atttaaagtt
    27421 tttctaccac ttatatttct tcctccagct agtgctctta tttcaccatt tttataatct
    27481 aagattacca ttgcagcttg cggttggcta actccatcag gaccgaccat atgtcctggg
    27541 aaattgctat ttctatcaaa ttcttcttct aaagcatctt gcattttagg gtctatagtt
    27601 gtgtttacaa ttagaccacc attgtagaat aagttttgag cttcatcatt tgtatgtcct
    27661 tgttccatta aagcatttat aacttcactt tcgataaggt cttctacaga tgaagaaact
    27721 ttatctgatg attgaggcaa ttttatttct attttagctt tgattgcttc atcatattca
    27781 ctttgtttta tatatttcaa ttctaacatt ttctttaaaa caatttcttg tcttttttta
    27841 gcttcaggat tacttacagc ctttctaaca actaatgtac ctgcttttaa ttgtctataa
    27901 gtatcattag atataagctc ccaagatttt attttttcaa tcatatcaaa gtccacttga
    27961 gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa ataataattt attctctaaa
    28021 tcctccttag tttcatttcc ctctaactta gaagttttat aggctgaaaa tcttgatgga
    28081 tttttagttg aaccagcaag aagtgctgac tctgctagag taaggtcagc tgcagactta
    28141 tcaaaataac cacgtgcacc agcctcagca cctgctagac ctctaccaac gaagaagtta
    28201 tttaaataag tttctagtat tttatcttta cttactgttt tactcatctc atgtgcatag
    28261 taaatatcct ttattttacg aggtatagtt tgctgttcag ttgttaacag cattttggat
    28321 acctgcatag gaatagtact accaccttgc ttagtacctg taagggtctt taatactgac
    28381 ctaaataaac ctttaatatc gacaccctta tgctcataaa aacgctcatc ttctattgca
    28441 attacagcat gttgaagata aggggaaatc ttatctaatg gaacgggtgt ctttttattt
    28501 acacttggag ctgtagataa tgtttttcca ttagcgtatt taatagtagt tgtttggttc
    28561 gtctgtttat ctaaaacagc ttctgtaact ggagttacat ctcttagtga cgcaaatact
    28621 agaccagcag tacctgctgt accaacaact agtaaaacta aaaatactat cccaaatact
    28681 cttagttttt taaatttatc gcttttttta gattttttct tgttttttgt tgaacgagtc
    28741 ttgttagctg aagttctatt aattggttta tttgtattac cagatgagct aacttttttt
    28801 ctcctgattt tatttccatt tttatcatta tcgttattca taagatttta acctccttac
    28861 ttaatgcaat attataacac aaataggagt agttttgtaa ttaatagaaa aaatgaattt
    28921 gctataatat tctgtacatt gtatatgtat aaacgtaata tattaaaaag tctatatttt
    28981 tattttattt atttttgaca aatattataa aaattatgcc aaagctacat ataaataaat
    29041 aggaggggat aattttgaat agaatagtag atagaaaaac aaaaaatgaa tttagaaaaa
    29101 tcatagctat ttttttagta attcttttct tgtctgtatt tataggcagt tttatatata
    29161 tagataaaac tttaagaccg acaataacag ttttagcaga gactaaagct cttgaactag
    29221 caaataggtc gataaataaa gctgttgctg agatggtaga aggaaaaata aattatgaag
    29281 atttaatgga tatacaactt gataataatg gtaaaataac tatgatacaa gcaaacacta
    29341 taatgatgaa tgaaattgca tctgcaatag ctttagaaat acaagatgaa ttaaaaaagg
    29401 ataagacagc aagttcttac atacctatag gaacagcatt aggaagtcct atattagcta
    29461 aatacggtcc taaacttgaa gtatctattg aacctatagg aacagtatca gtaaacttta
    29521 aaacggaatt tgagtcttca ggtataaatc aaactagaca tagaatatat ttagaagctc
    29581 aaacacaagt taaagtagta ataccactta taacttcaac aaaacaaatt aaagctcaaa
    29641 tacctatttg tgaaactata atagtaggtg atgttccaga aagttatgta aatattccag
    29701 aaaaaaatct aggaaatgtt ttacctaaca ctggaaagaa taccaac
//
LOCUS       DAFKMX010000024        29557 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFKMX010000024 DAFKMX010000000
VERSION     DAFKMX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.024"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            304..1971
                     /locus_tag="LZB08_000946"
     CDS             304..1971
                     /locus_tag="LZB08_000946"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBM7314460.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(2067..3386)
                     /locus_tag="LZB08_000947"
     CDS             complement(2067..3386)
                     /locus_tag="LZB08_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7314461.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQATEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(3427..4173)
                     /locus_tag="LZB08_000948"
     CDS             complement(3427..4173)
                     /locus_tag="LZB08_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBM7314462.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLEAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(4417..6426)
                     /locus_tag="LZB08_000949"
     CDS             complement(4417..6426)
                     /locus_tag="LZB08_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7314463.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(6864..8297)
                     /locus_tag="LZB08_000950"
     CDS             complement(6864..8297)
                     /locus_tag="LZB08_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBM7314464.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDILQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(8313..9737)
                     /gene="ascB"
                     /locus_tag="LZB08_000951"
     CDS             complement(8313..9737)
                     /gene="ascB"
                     /locus_tag="LZB08_000951"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBM7314465.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDVWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYNRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(9824..11740)
                     /locus_tag="LZB08_000952"
     CDS             complement(9824..11740)
                     /locus_tag="LZB08_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7314466.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(11814..12674)
                     /locus_tag="LZB08_000953"
     CDS             complement(11814..12674)
                     /locus_tag="LZB08_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7314467.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(13028..14215)
                     /locus_tag="LZB08_000954"
     CDS             complement(13028..14215)
                     /locus_tag="LZB08_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7314468.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFDNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKF
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSGEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(14229..15620)
                     /locus_tag="LZB08_000955"
     CDS             complement(14229..15620)
                     /locus_tag="LZB08_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBM7314469.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(16215..17915)
                     /locus_tag="LZB08_000956"
     CDS             complement(16215..17915)
                     /locus_tag="LZB08_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7314470.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(17887..19290)
                     /locus_tag="LZB08_000957"
     CDS             complement(17887..19290)
                     /locus_tag="LZB08_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7314471.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEVELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(19555..20046)
                     /locus_tag="LZB08_000958"
     CDS             complement(19555..20046)
                     /locus_tag="LZB08_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7314472.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEADLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(20072..20884)
                     /locus_tag="LZB08_000959"
     CDS             complement(20072..20884)
                     /locus_tag="LZB08_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBM7314473.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(20897..22048)
                     /locus_tag="LZB08_000960"
     CDS             complement(20897..22048)
                     /locus_tag="LZB08_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBM7314474.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            22318..22959
                     /locus_tag="LZB08_000961"
     CDS             22318..22959
                     /locus_tag="LZB08_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBM7314475.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            22985..24529
                     /locus_tag="LZB08_000962"
     CDS             22985..24529
                     /locus_tag="LZB08_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7314476.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(24729..25355)
                     /locus_tag="LZB08_000963"
     CDS             complement(24729..25355)
                     /locus_tag="LZB08_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7314477.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(25490..26206)
                     /locus_tag="LZB08_000964"
     CDS             complement(25490..26206)
                     /locus_tag="LZB08_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBM7314478.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            26632..27846
                     /locus_tag="LZB08_000965"
     CDS             26632..27846
                     /locus_tag="LZB08_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBM7314479.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            28460..28693
                     /locus_tag="LZB08_000966"
     CDS             28460..28693
                     /locus_tag="LZB08_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314480.1"
                     /translation="MKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLYL
                     EIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVD"
     gene            29322..29429
                     /locus_tag="LZB08_000967"
     CDS             29322..29429
                     /locus_tag="LZB08_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7314481.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            29431..>29557
                     /locus_tag="LZB08_000968"
     CDS             29431..>29557
                     /locus_tag="LZB08_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314482.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRID"
BASE COUNT         9842 a         5157 c         3412 g        11146 t
ORIGIN      
        1 agatattgtg tatatctact agaatttaag tatcatctat tatttgcatt taataataca
       61 acaagatatt ttttgaaata agagttaaat aatagtacaa ccttttattg catttaaata
      121 ttatcaaaac aaaaataaat gtactatact atttatccca tactaacctc actattctat
      181 atacaattat atgtttatac aaataatcat ttattttata ttttagcttt aatatcagtt
      241 taatacttga caatttgttt aaacatatta taattaactt ataaattaca ggaggtatca
      301 agtatgaaaa attggtggaa aaaagcaact gtctatcaaa tatatccaaa aagtttcaaa
      361 gatagcaaca atgatggtat aggtgatata aatggtatta tagaaaaatt agattatctg
      421 tattctttag gtgttgattt attatggttg actcctatgt acgtttctcc tcaaagagat
      481 aatggatatg atatagaaga ttattataat atagacccaa aatatgggac aatgagtgac
      541 tttgaaaaac tcttaaaaga agctcataaa agagacataa aaataatgat ggatatggtc
      601 ctaaaccata catctactga acataaatgg tttaaagaat ctaaaaaaag caaagacaat
      661 ccatatcgtg attattattt ctggaaagat gctaaacctg atggttctgt tcctaataac
      721 tggatatcca gatttagtgg aactgcttgg aaatatgatg aaaccactaa ccaatattat
      781 ttacatttat ttgaagaaac tcaagctgat ttaaattggg aaaatgaaaa ggttcgtgaa
      841 gaatgttaca aaatactaga attttgggct gataaaggta ttgatggttt tcgtttagat
      901 gtagtcaacc tactctccaa aacacctgga ttacctgatg accctataac aggaccaaaa
      961 ggagatggaa gaactcacta cgctgatggt cctcgtatac acgaatattt acataatatg
     1021 aatcaaaaag ttttcaaacc taaaaatata gttactgtag gtgaaatgtc atctactaca
     1081 cctgaagagt gtatcaacta tactagagaa aatcgagaag aactatctat ggtttttagc
     1141 ttccatcata tgaaaacaga ttataaagga ggcgcaaaat ggactaatga aatgttctct
     1201 ctggataaat taaaaaaagc acaatctgac tttcaatata aaatgtatga aggaaaagga
     1261 tggaatgctc tattttactc taatcacgac caacctagag ctttatctag atttggagat
     1321 gatagatttt ttcgtcaaga aagtgctaag atgttagcca taacattatt tggtctacaa
     1381 ggtactacat acatatatca aggtgaagaa ataggtatga caaatgccta ctttactaaa
     1441 atagatgaat atgatgatgc tgaaagtaag aatgcatact atcatatgat taaatctggt
     1501 attgatgaaa aggaggcatt gcttatactt caacaaaaat ccagagataa tgcccgtact
     1561 cctatgcaat gggataatac tatttataag gggttttcta atcataaacc gtggttaaaa
     1621 gttaataatg acaatattag tgtagaaaat gagcttaatg attctcgttc agtattttat
     1681 acttatcaaa gattaatcaa atatcgcaaa caatatgata tttttacaga aggtacttat
     1741 agattgttag atgaaaatca taaaaatcta tttgtttatg aaagagagta taaaaaccaa
     1801 aatcttcttg ttataagtaa ttttactcat gataatgtag tatataaatt acctgaaact
     1861 tatttgaata aattaaacta cacaatactt gaaaccaatt acactagaga tataattgaa
     1921 aataaaatgg aaataaaacc ttacgaaagt ataatgatat attataaata aagttattta
     1981 tgtaaaaatc ggtataacac cctggtgtta taccaatttt actaaattca aagtaatatt
     2041 tatagaatct ctatagatta caataattat ttgacttcta catcattata tttctttgaa
     2101 tatactttag tcatatagaa atatatagga actacaatta tcatcccacc taatccccaa
     2161 actatcttca taggttcatc aatacctgct tttatcagta ataatatact tataataact
     2221 gcaaatattg gtataattgc tcctccaggt attctaaaat ttacatcttt accagcatac
     2281 ttctttctca atattagtac agctagacat gtaggaatat attgaataaa tctagataca
     2341 acacttattt gagataatgt tgtgaatgaa cccgaccatg ctattagtaa agttcctatg
     2401 acagatacta taattgctat ataaggagct ccatttttat tagtttttct tatagactta
     2461 ggcattaatc cttgttcaac caaagcagca gcagaccttg gtgtaaatat ggatgatgca
     2521 atatttatac ctccaacaga tactatagtt cctattgtaa ttatataccc gcctatagga
     2581 cctaacatct taatggcagc atctgcaaca ggagtagctg accctgctaa gccattaccc
     2641 aacaccccta tacttattcc cagtataagc atatatatta ctgatactac aaacattacc
     2701 attaccaatg ctttaggtac atttttctta gggttctcca tatcttttgc tgcaacagct
     2761 aaagattcaa atcctgtaaa agcataaaat aaagttatag atactgctac aaaagctgat
     2821 gacgcacttt gacctttttg tattattaca aatggggtaa aattagaacc ctttataaag
     2881 aataatccta cagctataaa taaaactatt ggtaataatt ttcctatagt catgatatta
     2941 tttaagtgct tagattgttt cattcccata aaatttataa tcccaagtcc tacaactaat
     3001 ataccaatta ttatattttt aataagtggt tctgttgctt gtggccatac agatgatagt
     3061 gttacagcaa agaaatttgc catagtagcc caagcaatca tactaactat ccacttcata
     3121 aatccaactt caaatccaac aaaatctcca aatgcctctt ttgcataaag atagggacct
     3181 ccatcagtat caaaccaact acctgcttca gcaaaacata aagcaataga aacagctaaa
     3241 agcatacaaa aaactaatac taatatactt gctggaccta tatctgtata agcatcacca
     3301 ggtaacccga atatacctga ccctattatt gcattgattc ctaatagtac aatactccaa
     3361 aaacctaact taatgccttt ttccattgtt attcccccca aagccaactt aatatatttg
     3421 tattttttat ttagatgctt ctttaactat tgccataaat atttttttca tattatcata
     3481 atctctagtc atcatttctg gatgccattg tatccctatt acaaactcac ttccttcctt
     3541 ttctatagct tcaattaaac cgtctttaga tgtagctgaa acaatatatc ccggtgctac
     3601 tttgttgaca actaagtgat gaaaactatt aactaaaact tctttttctc ctagtatttc
     3661 atataatttt gtcccctcct ttatctgtac tgtatgtgta ggtacattag atagatgttg
     3721 ctgattgtgt ttaatataag caccttcaat taaagataaa tcttgataca aactacctcc
     3781 ttcagttaca tttataattt gttctcctct acatatacct aaaactggtt ttttcatatc
     3841 taaagcatgt tttaataatt tcatatcaaa actatctctc tttggtgata ttgcctccaa
     3901 cttattatga ggttcttctt tccatatgag tggatttaca tcttgtcccc cagatagtat
     3961 aagcgcatct atattagata cctgctcctt tataatctca tcatcatata caatcggtac
     4021 aatatatggt atagctttac acataactac tgattgtata taatcattat ttacataagc
     4081 cctttcatag ccaggaaaca ttcctccttc atcaattagt aaattacctg atattcctat
     4141 tattggatat ttctttttag ccaatttctt caccccttaa cttatttgtg aacaataaaa
     4201 aatgctactt ctcttaaaga gcaagtagca taccaagatt ttatttattt gaacattata
     4261 atttatttta gaaatacttt ttttgaatac gagtaatata aataacttta tttacttata
     4321 ttttatttta atcctgttta gtttgacttt tttaaattgt tttaagtaca actaataggt
     4381 aaaataggca tttagatttt cctttttatc aacctattaa atactgttta atatattttt
     4441 tccccttcta tttaattcac ttccctttct accagattca catattatca aatccagctc
     4501 ttttaacata ttaagtattc cccttacctc accttctgtt atattgtagc ctaattcatt
     4561 tattgaatta acaataccat ttctacctat atttttatta agattattgg cattttttaa
     4621 acactccatt actaacttta ctttctctat agatgttcta tcctttaata tacttatttc
     4681 tttactaaaa tcttcagtta tatttaataa gttatgaggt aaatcattta ttaaaataac
     4741 atcctcagaa ctcataatgt taatatacat agcagtattt tttaattctc ttatattacc
     4801 tggccattca tatgcattaa taatgtcttg aacatcttga ctaattgctc tcttcctttt
     4861 cataaaatgc tttaacataa cttctatatc ttcttttcgc tctcttaaag gtggtatatt
     4921 tatgggcaat acatttatcc tatagtataa atctgctcta aatctatctc cttttatcat
     4981 ttctaataaa tttttatttg tagccgcaat tactctaaca tctatatcta ttacattttc
     5041 tcctccaacc ctcattactt gattctcttg taatactcta agtaatttgc tttgtaagta
     5101 tataggcatg tctcctattt catccaaaaa tatagttcca ttgttagcct gttcaaataa
     5161 tcctttttta cctcctttta aagctcctgt aaaagcacct tcttcatatc caaaaagttc
     5221 actttcaagt aaattttctg gcatagctgc acagtttatc gctataaatg gctgactatg
     5281 tcttggtgat gcattatgaa tagactgagc catgagttct tttcccgttc cactttctcc
     5341 cgttataaga attgtataat cagattcagc tattttcata cctaatttct tagttctttc
     5401 catcttcgtt gaaactgttt ttatatcatc aaaagtatat ctagcaactt gtcccttttc
     5461 tctaagcttc tttgataggt tttgctccaa ttttcttatg taagttattt cttgaataca
     5521 aaaataatat ccagtaaccc ttccaaaact ctctatatta tacttattta cgtttaagta
     5581 cttattatta aattttataa cttcatcatg tgccttactc aaactaaaaa tctttttaat
     5641 attctcattc tctaattcat ctatattttt ccctataata ttactttgaa tatctagtat
     5701 atcccctaaa ctattattgc aaataattat gtttccctta tctgatacca ttgccatacc
     5761 taagtttgat aaatttatta tagaatttag ttcatctgct tttatagtaa gttctttgta
     5821 tttagtattt attcctgaat ataaactaat tatttcatct gtatacttaa ttagcctctc
     5881 agtaatttct ttatcatcca gtttgagttt tgctattatt tgaataaacg ttgaactatc
     5941 aatataccta tggcctaaat caataacttc ttctatatat tttggaacta aacttgactc
     6001 tcctggcgtt atagctatct taatatttct atattcttca ttgggattgt atggtataaa
     6061 attcaaatgg tttattccta tattgtataa attagatatt gtttccaata cagtatgctt
     6121 attatcgttt actactaata catctattcc ttctggcaat gaaaataact tatatatatc
     6181 cttttgcttt atgctcctat ttatagttat tatctttgag acatcttcta catatttttt
     6241 tatttttaat actctatcat ctatcattac caatattatg tcatctttta ttaattcctt
     6301 ctctgttatt ttatttaaat aataattttt tatcattact ttatctttaa atatcatttc
     6361 tagattttta tttaagaaac attctaattt agacaaccca tcagttacta ctgctattgt
     6421 tttcatttga ttactccttt taaaatatac tctactcttt tataatatac atctgatttt
     6481 cactttaaca gcacaaaaaa ttgtacattt agtatactaa catattcctt tattagtttt
     6541 atgtccctaa atatcatagt tgcaatatct atcatatttc ctaaacttct atagagcaaa
     6601 aattgtgcca aattaataat tttcttaatt ttattctaaa gttcctcaat agtatttaca
     6661 gacttagcac ttatagctta taagtcttta tattattaat ctatttttat aggttaattg
     6721 tacaaatcat atatcacttt tacccattaa atgcattata aaaaagatgg aataacgatt
     6781 cgttactcca ccttttttgt aataaaaaat tgtatatata aacactattt actactcttc
     6841 taagtacaaa cagtatctac atactataac ctttcaccat tagattctat tacttgttta
     6901 taccagtcaa aactcttttt cttaatccta gacaagtctc cacttccatc atcatactta
     6961 tccacgtata taaacccata tctcttggcc atctcaccag tagatgcact tactaaatct
     7021 atacatcccc atggagtata tccaaataaa tcaacaccat cttctacagc ttccttcatt
     7081 tgctctatat gtgcttttaa atactctata cgatagtcat catttatcac tccattagat
     7141 tcttttttat cataagctcc taaaccattt tcaactacca tcataggtat tctatatcta
     7201 tcataaattt catttagtgc atatctaagc ccttcagggt ctatttgcca tccccattca
     7261 ctagacttaa gatatggatt tttcactcca cctaatatgt tgccacttgt attatcttct
     7321 ttagaatcac tacttacaca tgtagacata taataactaa atgtataaaa atctacacaa
     7381 ccttctttta aaatttctaa atctcctttt tccattttta tttcaatatt attttctcca
     7441 aaatatctat tcatataagt tggatattcg ccacgcactt gtacatctcc acagaaataa
     7501 ttcattactc tactatctct ttgagtttta actgcatcat ctggattaca agtataagga
     7561 tacattgttg caaatatttg catacatcca acttgataat ttgaatctat ttcatgagct
     7621 aacttaacag ccttagcact tgctacaaat tgatgatgta acccttgaaa acgaagttga
     7681 agtatatcta cttggtttat aaaatctgta gtcccttcat ttaatattcc ttgtgataag
     7741 tagcctccca taggcattgt agctgaattt atttcattaa atgtcagcca atattttact
     7801 tttcctttat atcttttaaa tattgtttca cagtaattca tatacaagtc tattacatct
     7861 cttgaaaccc atccattgta tttctctgtt attgcaaatg gtatttcata gtgagatatt
     7921 gttattaatg gttctatatt atattttaaa cattcatcta tcacattttc ataaaattta
     7981 agtcctgctt catttggttc tttctccatt ccagttggaa atattctagt ccatgctatt
     8041 gaaaatctaa ataccttaaa tcccatttct gcaaataaag ctatatcttc tttgtatctg
     8101 tgataaaaat ctattgcttc atgacttgga taaaatgttc ctttttctat aactctcgtt
     8161 attcttttac ttactttttg agaacctcca gtacacatat ctgaacaaga aggacctttc
     8221 ccatctaaat tccatgctcc ttcacattga tttgctgctg ttgctccacc ccataaaaaa
     8281 tcttttttaa atgacatacc tatatcctcc tattataatt cttcaccatt tgattctatt
     8341 acttttttat accaggcaaa actcttcttt ttactgcgtt ttaaagttcc atttccatca
     8401 ttatctttat ccacatatat aaatccatag cgcttcttca tttctccagt acttgcactt
     8461 actaagtcaa tacatcccca tggtgtgtac ccccataaat caacaccatc taactcaaca
     8521 gcatctatca tggcttttat atgagcacgt aaataatcaa ttctataatc atcatttata
     8581 tttccatttt catctggatt atctactgct cctagtccat tttcaactat aaataatggt
     8641 ttttgataac ggttatataa agagttcata gttattctaa gtcctaatgg gtctatttgc
     8701 catccccact cacttgcatc tagatatgga tttttaagac ttgcaaaaac atttccttct
     8761 gttaaaatat ttacttttgg gtctgcactt gtaagtcttg atgagtaata tgagaacgaa
     8821 ataaaatcta ctgtgttctc tttaagtatc tttttatcat cttcttccat cactatattt
     8881 attccttttc tctccaattc ttttaatgca tatgttggat attctccacg agattgtaca
     8941 tctataaaaa agtaattttc cctatctttt tctatagatt tccatacatc tagtgggtta
     9001 caagtattag gataagtatt tcctgctgct aacatacacc ctattttaaa ttcttgattt
     9061 atgttatgtg ctagtttagt aactaacgca ctcgcaacta attgatgatg tgcagcttgg
     9121 tattttattt gttcttcatt ttctccctct tcaatataga gacctgcacc tataaatggt
     9181 aaatgaagta tcatgtttat ttcattaaat gttagccaat actttacctt acctttaaac
     9241 cttgtaaaaa taacttcaca gtattttaca tataagtcta ctagcttacg atttctccat
     9301 gctccatatt tttttaccaa atacatggga acatcaaaat gacagattgt tactactggt
     9361 tctattccat actttaaaca ctcatcaaat aaatcttcat agaattttag tccctcttca
     9421 tttggaacat cctcaactcc tgttggaaat atcctagtcc atgctattga catacgatag
     9481 catttaaatc ccatctcagc aaataatttt atatcttctt tataattgtg atacatatct
     9541 attgcttcat gagctgggta atagtgttca tcatcacatt caaacatttt cattttccca
     9601 gccaaaactg gaaatctatc ttttccttgc ggtgtaacgt caacattagc tagacctcta
     9661 ccatcttctt tatatccacc ttcacattga tttgctgctg ttgctccacc ccataaaaaa
     9721 tcttttctaa gtgccataat tctacctcca ataatttatt tatagtaact ttattttaaa
     9781 ataaaaatac atatatataa ttcttaatta atagaactct cttttacgct aaaacagtta
     9841 tcaatttctg actattattt acatgctttt gtgtattttc tacaatatcc acataatcag
     9901 aataattagt aacaactact ggtgtttcta agcaatatcc ttcttttgaa atactttcta
     9961 tgtcaaaagt tattaatgtc tgaccttttt taatcttatc cccttgatta acaaaagatt
    10021 tgaaatattt accttccaac tgaatagtat ttaaccctat atgtatcaat agttcacaac
    10081 cattatcaga tataattcct attgcatgtt ttgttggaaa taaagtcatt acagtcccat
    10141 caaatggtgc cactactttt ccttctttag gtgttattgc tacacctttt cccaatatac
    10201 ctgttgaaaa cgctgcatct tgtatctgct ccaaagcctt tacttcacct tttattgggc
    10261 tatatactac ttctcttttg tttttttcta taacgccttg cccattatca tctttagaaa
    10321 tttctactat agcatcatct ttccaaaaaa ataatgttaa aacaaatcct actacaatag
    10381 ctactattat tgctattata gttgcataaa cacctttcat atctccagtt gttggattta
    10441 tataattagg tataccaaat attccaagtc cacccattac ataacttttt acattcatta
    10501 tccccattac agctcctgcc acaccaccac ctacacagct atatatgaat ggtgtttttc
    10561 ttggtaaagt tattccatat attgcaggct ctgttacacc acatatccct gaaataactg
    10621 caggtataca cagctctttt atcttcttat cttttaattt aaagtacatt gctgctacta
    10681 ctgctgtttg tgcaaaagat ggtacaaatg ctcccactaa tattgtgtca aaccccatag
    10741 tcataaggtt tgacatagct attggtacta atccccaatg tagaccaaat attacaagca
    10801 cttgccacaa taaaccagtt aatgcaccac ataaaattgg tgaaaagtta aataaagcta
    10861 aaaatccatt tccaactata ttagaagcat aggtagctat aggccctata accataaatc
    10921 ccaatggtaa agaaactaat aaaactgcca ttggtacaaa gaaattcttt actacatctg
    10981 gtattatttt agcaaacact ttctccaact tagctgctaa taatactatt aaaattactg
    11041 gtactactgt tgaagtgtag ttcattgcta ttactggtaa tcctaagaaa gttatatata
    11101 ctggtgattc aaatattgtt ccagtaaata gattgtataa aggttgacct gtagatgcta
    11161 gagtatctaa ttgaatacct gaatagcata aaactgcacc tataagcatt ccaataaatg
    11221 gttttaattt gaatttctta gcagctgtat atccaagaat tattggcatg tactggaaca
    11281 ttgcatctcc aattgcattt aacataatat atgcaccatc agtttctcca tacaaaccca
    11341 atgctgtaaa tactgcatta aatcctttta tcataccagc agcacacaga actcctaata
    11401 ttggttgaaa aactcctgat attatgtcta taaacttatt aagtaatcct tttggcgcat
    11461 ctgagtctga attatctatt gtatctcctc ctaatccagc caaatcgcac acaacttcat
    11521 aaacatctgg aacatgatta ccaataacta cttggtattg acctgcactt ttcattacag
    11581 taactatacc atccattttt tttagcacat catcatttgc tattgactca tcttttaatt
    11641 taaaacgtag cctcgttata cagtgagata aactgctaat attatccttt cctcctacat
    11701 tcgcaatgat ttcttttgat agttgttcat actttctcat tttttctcct ccattttctt
    11761 tttgaaatat gaaggtttgg ccaacttaat gtcaccaccc aatatatata aacctaatta
    11821 ttaggattta tatctgactt aatactttta cttaccactc tttcaacgtg aatagttaag
    11881 tataattttt cctcatctgt taaatcgtag ttgtattttt tatgtataaa ttcatccaac
    11941 ttttctatga atttataagc atttttatat ttaactttta ttacatttaa taaatcatct
    12001 tcattatttt catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta
    12061 ataaatctat gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc
    12121 atatttgaaa tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc
    12181 atttgtgcat taactatatg cagtgctata aatccagctt catcttctga tagtctaatt
    12241 tcaaattttt tttctatata atccaatgcc tctaacccta ttttaaattc actcttataa
    12301 aatcttttta tatcccaaag taatacattt tttacactta ctccttcacg agctctttct
    12361 attgcagtat acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct
    12421 agtttttctt tagcatataa aattatttca tctgataatt ccatatatct tataggaata
    12481 tctgctatta gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta
    12541 tcaattttat cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc
    12601 ataactacta tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt
    12661 ttttcaataa tcattgtgat gcccccttta ttagcattaa gttaaaaaat aaatcttttt
    12721 taattattta gaatatttaa aatgtaattt attacataaa aaaaaaccta tttattacac
    12781 attaaataga ctcccatgag tcctgagtaa taaataggtt tagcttgtat ttaaacaatc
    12841 actatccgtt tgtattttat tttaattatt atttattata tgttagcaaa tttatttttt
    12901 agtgtcaact ttttctcaat attttaattt taaataaatt tatataaatt gaatcttcaa
    12961 agtactattt ttatcaataa acaaaataaa aaaggtatta ctttctttaa gtaataccca
    13021 tttattttta atttaagtgg tttttcttaa tctcatcaag tagtccatct tctaaataaa
    13081 tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca taagcttcac
    13141 cagaatttgc ataatttaga aattcttttt catgaacata taccattgga cttatatcta
    13201 aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata tctgtagaac
    13261 ctactggacc atctttacct tctaatatat ctgttatacc tgcctcaatc aaattattct
    13321 ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt tcaaagtatc
    13381 tataagaaaa cttagctcct gtcattaact ctgcaccttt tgcacagttt ataacttttt
    13441 tagttacttc atctaaataa cttctttcag cagctcttac gaaaaactta caggctgctt
    13501 tttctggaac tatatttgga gcctctcctc catttgaaac tataccatgt attctaacat
    13561 ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt actgcatcta
    13621 gtgcattgac tcccatatga gggtgactag ctgcatgtgc tgatacccct tcaaaactaa
    13681 actctaatga atctatcgcc aaagccttac agtttatatt attaaaagct tctatatgca
    13741 tctgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca cattttcctc
    13801 ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa ttttctttta
    13861 acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta tgtccacaag
    13921 catgagctgg ttctttattt tctccatatc caggaagtgc atcatactca gctaaaaatg
    13981 ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc atattacaat
    14041 atggataaac tgtatcaaat ccatattcct tcattttttc tactaagtat tttgatgata
    14101 tgtattcttc ttcccctaat tcaggattgt caaatataaa attacaaact tcttcaattt
    14161 cttttccaag ttcattctct atctcataag ctcttttctt tatactctct atcataattt
    14221 acctcctact agaatcctag atataatcct attgtcaaaa atgccattac aagaattgtt
    14281 aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat attggctact
    14341 gctagtgctc ccattaaaac acctgatgta ggtgttatca agttagttac acctatacca
    14401 aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc aagtggtgca
    14461 agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa tgctattaat
    14521 ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt taataaattt
    14581 gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat tacgattcca
    14641 cgtgcaaatg ctatacacaa tcctgctgtc attaaatctc ctgctccact tataaatgaa
    14701 ttaactatat catcttgttt aagacctgat gctatacctg atattatacc tatagctgta
    14761 aatatcattg caatttcagt tatataccat cccaacttta aaactccata tatcataatc
    14821 gccataccta ttgtgaaaat tgctattacc atggcttctc tttttgtaaa ttcctttata
    14881 tttgtatcac tattaaccat tgattcttga tttagtgata tatcgtgtac taaagatttt
    14941 ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga tatagccata
    15001 tatattataa acataacaac tctaaactct attccagaac ctggtgctat ttgtgataat
    15061 gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc agctgttgct
    15121 cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt cataagtgga
    15181 ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt cccacctagt
    15241 ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat tttatttaca
    15301 gtatgagcta taacagcttc tatagctcct gttttattaa ctatcccaaa agctcctcca
    15361 actattaaaa caaatccaac tacttcagca gcatctataa acccgtttat aggtgctgta
    15421 aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc agctacaaca
    15481 actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat ccaagtaaga
    15541 attgccatta acactattag caaaaatatt attgtaagtg tctgtggcat agtaaacttt
    15601 ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata ttttattatt
    15661 gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata catgttgagc
    15721 taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag atagctctcc
    15781 atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag tcccagcact
    15841 agcttcggac actagcactt cggcacttac tccttttata ttgcctcatt ggtttccaac
    15901 ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgtttgt tagatgaggt
    15961 aattcttatt aatttttatt aattcaaagt aattgtagca taatatgtcg agattgtaaa
    16021 tattttcagt ataataattc ttactttatg atttcattcc atttttcaca aataattttt
    16081 tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt ccacaaattt
    16141 taaattatta taataccttt ataataaaaa ataaaagcta tgtaaactta tcaatttaca
    16201 cagctttata tacattaatc tactccaagt acagctttaa gctttgatgc tattgtaata
    16261 actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc acttgcaccc
    16321 aattcattta cccagaagcc atcttcatag aatccatcaa ttgaatttag ctctacccta
    16381 agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa accttctatt
    16441 atagaatgta caagctttgt atctttatca tctacttcct cttctactgc attgttgtat
    16501 gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact ataaagctta
    16561 acttcttctg agcttcttcc tggtgttttt atatcaaact tcttgattgc ccatgtaaat
    16621 attatataag taactataaa aaatattggt atcaaaaata ataatggtaa cgcatccact
    16681 ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat tatagaaaca
    16741 ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc tacaaaatac
    16801 aataatggtg caacaaatag gaatgtatac tctattggtt ccgttacccc taccaacatg
    16861 gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc attatcagcc
    16921 aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa taaacgacct
    16981 gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg agctaatctt
    17041 atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc tactcttgta
    17101 tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc tttatccatt
    17161 gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc aagtttagat
    17221 aatgccaacc ctactggtgg ccatataaag tacattgctg tacccaatac tattgatgct
    17281 ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc actaggaagt
    17341 tctatgtcaa caaatttatt atgtactgca ctagctacaa atccagctat tatagctcca
    17401 aatatactac aatcatatga aaagattcct aagaaagaac catataaaga acttcgacta
    17461 tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac tgtatctgaa
    17521 tttataccat ttgctgctaa caatgtactt ataactgtgt gcattcctat aaataaaaca
    17581 attcctccta atgcacccca tcctttttcc tttttagata atccaaaggc tatacctgta
    17641 acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat tactttaaag
    17701 aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc agaaacgttt
    17761 tccatatttg caaaagctga ccctaatcct ataaatactc ctgctacaac aagtaatata
    17821 acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt attatttcct
    17881 ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta ttgctttgta
    17941 taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat ccccctctat
    18001 tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag ctccttttgt
    18061 aaaaagttcg acttcttttc caaaaccttg tttatatgca tttgataatt tatttattaa
    18121 ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa ctattaaatt
    18181 ttcttttttt ccaatttcat taattctatt agttataaac tcccaactta tacccttagg
    18241 atatctaaag tctattttca tattcaattc atttttctct aagaagatac tcattataca
    18301 tattgtagaa actcccatat ctacatcttc tttatacatc cctaattttt ttccatgatt
    18361 atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat catttagttt
    18421 tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc tgtgtggatt
    18481 tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttcat ctaattcttc
    18541 tatctctgta taataagcta atgattctac taaatctttc ttatcattag ttttaaaaac
    18601 agcctcaatt ttgtcacata caaatccatc ttctttgttt gactttatat caactaaatt
    18661 gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata aaataccttt
    18721 ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg gctgttcatt
    18781 gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac ccactatcaa
    18841 tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg catataaaat
    18901 ccctatcaaa ggtcctttgt tgtcattaac tcctctacca taaagatagt tatctttttg
    18961 acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca ccacatctat
    19021 atgattaagc acccctacta cttcttctcc ttctccatat tctatatgaa ttgcatatcc
    19081 atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct tatcgaaggc
    19141 atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat ctctaattga
    19201 aggtatttca actagacttc taatatcatt tatgatattt tcttgattgg attttaaata
    19261 atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct ttatattaat
    19321 tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt ttttcaatat
    19381 ttcaaatact tctctaagtt accataatga tacggtaaac aatcaacaga ttatgacaaa
    19441 atcatactat acctattact aatattttaa cttatctatt acgtcttttt taatgtaagt
    19501 ttattccaaa aaatacataa aaaaagatgt agccttagct acatcttctt atatctattc
    19561 aacttccatt attatgctat taatatctac atttccaggt tctatcctat cttttactgt
    19621 tctcttaaca gaatcagcat catccattat aactacagga gttattatat ttaaatcatt
    19681 tgacgttata aaatctaaat ctgcttccat tattaaatct ttagctttta ccttatctcc
    19741 tgcctttacc ttaacatcaa acggctttcc ttctagtgac attgtctcta aacctacatg
    19801 tattaatata tttgtattat cattactctt aatcattatt gcatgctttg tatcaaatac
    19861 attgactatt tcaccatcaa caggagaata taacttacct tcacttggta ttattgctac
    19921 tccatctccc atcatctttt gggcaaaaac tgcatctgga acatcttcta agttcactaa
    19981 atttccctta aaagggctat atattttctc aatcttattt tcttttttct taaatatatt
    20041 aaacataaca cactctccac attataaaat tttatgataa tatttttctt aaagcttttg
    20101 ctacaccatc atttacatta gtatcagtta caaaatctgc catagatttt acttcatctg
    20161 tagcatttcc catagcaata ccaagacctg caaatttaat catggatata tcattttcat
    20221 tatcacctat acaaataact tctttgctat ctatacctaa tatatcacaa aattttttaa
    20281 ctgcaatacc ttttgaagta cctttactca taacttcaaa gtttatcttt ccagaactta
    20341 ctacttctaa atcttcttgt tttttaaatt cttcctttgc ttctctcaaa gaatctacat
    20401 tttctgagaa aagtatacat tttaaaattt cattatcagc tttttctaat aaagtttcac
    20461 aacttgcatt ttctattatt tcaattctgt cttcttcttt atttttacta tttattaatc
    20521 tatatggatg ttcttcacct atttcattgt tggatataac tattttacag cctttaaaat
    20581 gtttgtttaa attatacttt tcacctattg tatagatttt ctttagagct tctttgctca
    20641 aaaccttctt atactcatat cccagtaatt taaaatatgt cccattagtt gatataatat
    20701 atatatcatc ccctaaaaca cttgtaaaat atttcatagc attataaggt cttcccgtaa
    20761 ctaaggctac cttgataccc ttttccatga cttcttttag tgctttaata ttttcttctg
    20821 atacttcaaa tcctttcccc atcaaagtac catccatatc agtacaaatt aatttatatc
    20881 gcatttgtat cacatcctag taagattttt ctaatatttc aacaaggtct tctttctttc
    20941 caaaaggtaa aaatcctaaa actggacagc ttgttatttg ttctaattgt tcctttgtta
    21001 cattaaactc agacattttt gtcttaagtc ccactctctt taaaaaactt tctatttcat
    21061 ttcctaaagt tttagctgct tcatcatctt ccactttttc taattcttgg tttagcattc
    21121 tcgctacttt ggcaaatttc tctatattat ccttatagaa cttttctgca aattttggga
    21181 acactatagc caaagcctca ccatgaggaa tatgtgtaac tcctcctatt atttcactta
    21241 gtggatgtgg tgctgctgct ccagcatttg ccagtgaact tcctcctaat gtatctgcta
    21301 ctgctaactt tgttctgtat tctatattat ttttatcttc cattacttta ggtagatagt
    21361 taatgactaa ttccatagat ttttcagaca ttatctctgt aaaatcatta gcatttggat
    21421 ttatataact ttcaaatgca tgggtaaaag catcaaatgc tgttgatgca gtaattctct
    21481 ctggcagagt taacataagc tctgggtcaa ctatacattc ttttgagaaa caattttgat
    21541 gaaatattgt atttttctcg cttcctctcg atattactgc tgcttgtgta acttggcttc
    21601 ctgttccaga agtagttgat acagctatta atggtaattc tttttcactt accttatcgt
    21661 atattttaaa tggatttgta tatgtactaa acatataatc ccaatctatt ttttctaaac
    21721 caaaagtcaa tgctaatact tttgcagtat ctatacttga acctcctcca acagctaaaa
    21781 caacgtctac agattctttt ttagctaatt caaacccttc gtttattaat tctacagttg
    21841 ggtttggctc tactctatca aagtgtacaa cttctatacc tttttctatt aaattcaatg
    21901 taactctttt atataacttg tctaatggct ctacatttgg agtagtaact aacaaacatt
    21961 tctttccata ttttcctatt attctaggta attcacttaa agttccttgt ccaaaattta
    22021 ttcttgatgg ttgataaaag ttaatcatat ttttcctccc ttaaattatt gccttcatat
    22081 tcaaattata catatcatag gatttttaag gcaatacctt cactgttaac tcttatttac
    22141 taaagcttat ggcaattatt agacaatcaa tgttttatta ttaccatatc attatgtaaa
    22201 ttttattaag tacccatgtt ataattttat aatcaatctt attgtataat tgacttaata
    22261 ataaaaatag agtatataca cttttttatg acaaatagct taaaggagaa ttaaattatg
    22321 aaatatttga tagacagtgc taatcttgat gaaattcaaa agtgtttaaa gtatggattt
    22381 aagggaataa cagctaatcc atctatgtat cttaaagaga aagtaaactt ccatgaattt
    22441 attgagacat gtgctaattt aaatccagaa atattaactg ctgaagtcat tggaaataat
    22501 ttatacgaat taattaattc atgtgataag atattaaata taaataaaaa cactataata
    22561 aaaataaatt tctctgaagt tggcttagaa cttataaaca tattaaatag taaaggtgta
    22621 aaaactgcat gtaccttgat tttcaatgta aatcaagcat cattagctat aaatgcaggt
    22681 gctgattacc tattcccttt tataggtaga aatgatgaaa atgggaatga tggtataaaa
    22741 aaccttacag aaatatgtaa ccttatatca ataaatggat ataatactaa agttgttgca
    22801 gcttctatta aaaatgtatt ccacttaact caagcatctc tttgtggatg tgattatgca
    22861 gctgtaacat atgatttatt taaaaaagct tcttttaacc agcttacaat agatggtgcc
    22921 aatacttttg aaagagattg gtccaaggtt aataaatagt aactaagatt taggagggtt
    22981 tgcattgaac aaaagattat tagatattgc tgaattatta ctcaacagct cagactatat
    23041 tactgtagat actattgccg aagaattaaa agttagtaat aaaactattc gtaatgacct
    23101 tgttatttta gatgaatggc ttttagagtt taatttatcc ctagataaaa aaactggttc
    23161 aggggttatt attctaggta atgaagatat aaaacttaaa gttattagag atatcaatga
    23221 taaatcaaat tgtatatatg catactctcc tgaagataga aaaaggtata ttctaagtaa
    23281 attatttatt aaaaattcta agtttaggat tcgtgatatt tgcaatgaac tacatgttag
    23341 tagagctact gtgcataaag acttggtagt tattcaaaat tttttgcaaa actttaaagt
    23401 gactctagtt agaaaaacca ataatggagt atatttagag ggaaaagaga aagatattcg
    23461 aaaagcaatt tttgaacttg ctactgttaa taaaagttat actgaactta aagaaatatt
    23521 attctcgaaa tctaatgaat gcaaagatac cccatcaaca aaaatattta aggagctatt
    23581 taactatgat tttgagaaat tatctcaaat cactctacac accttacaag tagaaaaaag
    23641 taaccttagt gatgagtatt atataaactt cttaatacat atagctatat gtattaaaag
    23701 aatttcaatt aaaagatata ttaacttatc tgaaggtttt tttagagaat tattagaaca
    23761 tgaatacttt gaaaaatcaa aaattttatg tgctaaccta agtgaagctt ttgacgtaga
    23821 atttattgat gaagaggtct gttatatcct tcttcatata gaaggattac ttaaatcaat
    23881 aaagaaaact actactcttg aacctcttaa aaatgaagaa attacagagt ataatctatc
    23941 tgaagcaata gctgttcatt ggggaagtat actaaattta aacttaaagg acgatttaat
    24001 attaataaag tctcttgcta atcaccttaa atcagttcta cacagaataa actatgggtt
    24061 tactattaca aatcccatat taaatgatat aaagagaaca tttccttata cttataaggc
    24121 tgctaaagag tctaatactg ttatcaaaca gttgatgaat tatgaagtca atgatgacga
    24181 aataggatat cttgctttat atttaatatc agcaatagat agaagcaaat ctcccctaaa
    24241 tactatttta atatgtcact gtagtgatgg tgtatctaat ttattagtcc aaaagttaag
    24301 ttttgaattt aatcagatta atatagttaa atctatacct ctatcttcta tttcatttac
    24361 gaactttgat gatgtggact taatactaac cactgctcct gtagactttg aacataatgc
    24421 tgaactgata aatataaatg ctcttttaag taagaatgat atttctagac ttagtacaat
    24481 tataaaaaaa ctatattctg aaaaaaataa aatattatct tataaataaa caaaaatagg
    24541 cttgtctcaa catagagata aacctatttt tcattgtcca aatgataaaa atatatttga
    24601 tttcatagaa aaagtgcctc atgaactaaa aaattcattt tgagacacct tcatttttta
    24661 tatttaatct ttttatttaa gcttttatat tttttaaact tttatattta aagtaaattt
    24721 tattattttt attgagcaca agcattattt atattattaa tttcttcagt attcttacca
    24781 aactttgtag ttacaaatcc tattgttttt attatgagta ataaggatat caatgaatat
    24841 atagatatca taaaattctt acttattatt ccagacgcta ctattataaa tccatctagc
    24901 ataaataata taactggcaa cttcagaaac ttaattacct tattaataag cattgcaact
    24961 aaatcagttc caccagtact acagcccctt attattgcta tccctatagc agttcctacc
    25021 aataacccac ctatcactcc tgctactaat agattgtcag ttatgtgagt ttgtggtatt
    25081 acttttaaga ataatgggcc cataagagtt atatataatg tttttattcc aaaacctttc
    25141 cctaaaaata ataatcccat tactaaaagt ggcatagata ttcctaatga tatattctca
    25201 acaggcattg ataagaatgt gctaagtacg actgataatc ctgttatacc tcctggagct
    25261 aatgtatgtt ctgcaaagaa aaaattaact gctaatgcca ttaaaaatgc tgatattgtt
    25321 atcagtaaat aatcttttat catttctttt ttcatttaat aacctcctat gattatattg
    25381 tttcagtata aaaaatcaat taaattagtc tagcttcctg tagtttttca taaaattaat
    25441 gcttccttaa aacattaaga aagcatctct tttatcatag tttattatct tattctaaac
    25501 caaactttgt agctgcttca tctatagctt ttctacaatt cttaacacct gctgttatac
    25561 catctttatc cttgaatatt gctgaagata atattacaac gtctgggtta cattctatta
    25621 atggctccat attgtctgct cttagtccac catctattgc taattcacac ttaggattct
    25681 tttcgtctat catttttcta gctcttttta ctaaatcaac agcactcttt ctccatcccc
    25741 agttatcttt tccatcagtt tcatcaacac catgaacgac tatttgcaat ctatctatat
    25801 catatatagc ttcatcaaca aaacataatg gtgtgtaaca tccaattgtt aatcctactt
    25861 tcataccaaa ttctctacag taatttatta tgtatgcaag aggagcacct aaaaaatgtt
    25921 ctgctggtat tatcaacaag ttacatcctg ctgctgctat tttttctata aataatctat
    25981 cacaatctct tgtgtatata tggcattcta ttggtttatc tgtcactggt cttattgctt
    26041 ctattatttg atgaccaccc ataagttgca tattttttaa gtcatgcata tctgctgcat
    26101 ctgaatgtac gtagtctaca cctgcatcca ttgcttcttt aactacggct gatatatgac
    26161 cgtaatttac atgtgctaat cctgctgcta tttttatgtg tttcatattt gtatacctcc
    26221 atattttact ttaattagta ttaaatatta gtttttgata tttttttaat attttttaat
    26281 ccagtattac ttttatttag cattactttt attgctgtat atatttcttt tatgaactta
    26341 ttctgctttt gttaactgtg aacttatttt gctttgtaaa ttaatattac actgatatat
    26401 tttttttcac aacactttga ctactcctta aaagtacctt tatattgtgg taaattatag
    26461 acattaattg tatataaaaa gttagttttt taccacattc ttatggcatt tattgtctat
    26521 actaactcaa ttgatatagc tagttcattt aatatataat cagactataa atataaattt
    26581 actcgataaa aagtaccatt cataacaata ttaatgaaag ggagatgtaa aatgagaaac
    26641 tgtattattg cccaatctgg aggccctaca gctgttatca atgcttctgc agttggtatt
    26701 tatgaaaaaa atttagaaac taaatacttt gataaagtat attttggtat caatgggatt
    26761 gaaggtatat taaacaaaaa tataatagat tcagactcct taaacaaaga taatgtatta
    26821 aatttaaagc aaacaccttc atctggtctt ggttcttgta gatacaaact ggctgattat
    26881 aaaataaatt cttatgatta tgatagagtt ataaatatac tagatgaata tgaaatagat
    26941 acattatttt atataggcgg aaatgattct atggacacag tgatgaaatt ttctcaatac
    27001 tgcaaaaaca ctacttgtaa aataaaattt ataggtattc ctaaaacaat agataatgat
    27061 ttattaggaa ctgacaactg tcctggattt ggttctgctg caaagcttat ttctacacta
    27121 ataatggaaa catacttaga tttttctata tacaaaaata atggtatatt tatagtagaa
    27181 actatgggta gagatactgg atggttagca gcttcttctt cactagctag acttaatgat
    27241 actccacttg ttgatttaat ataccttcca gaaattgatt tctatgaaga taagtttata
    27301 gaagatgtta gaaaaatatt taaagaaaaa aataaggctt atatagttgc atcagaaggt
    27361 ataagggata aatacggcaa ctttatatgc gaacaaaaaa cttctgctca tgataaattt
    27421 ggtcattccc aattaggtgg agtaagcaac tatctaaaga atcttatagt atcatcatca
    27481 attacaacaa gagtaaaaac cttagaactt ggtgtaatac aaagatgtgc tatgcattta
    27541 gcttcagata ctgatttgaa acaagcagct gaagttggtg catatgggat agaactatct
    27601 aaaaaaggat atacaggagt tatggctatc attaatagaa catgcaataa tccttataag
    27661 tatgaagtta gtcatactga tatttctaaa atagctaaca agacaaaata ttttcctaaa
    27721 gagtggataa atgaagaatg taattatata actgatgaag ccctaaatta tctatatcca
    27781 cttattcaag gtaaaccaga tatcaaatat aaaaatggac taccttattt ttcaattatt
    27841 tactaggaaa ttattataat ttgatataca ctcaattcta tcgctcttaa atacctaagt
    27901 ggctgttaaa taaaattata aattgttata tgacagtcac ttaatcgttt aattatttaa
    27961 ctgtttagtt ttttaatttt gtttaatact aatacaattt gttgtaataa tatctcacac
    28021 ccattttcat tttggataga ttataattag ttagacaata aatattttgt catgatatta
    28081 ttatatatag tgatacaaat agaaatactt ttatcatggc tagaaaaaca aagattacac
    28141 aaaggattta aaagtcaaac aacttcaatt aaaataaatg gttaaaataa cttctaaatc
    28201 aactacataa acaataaaag aacataaagt agttaaatct acaattataa aattagtaag
    28261 actataataa cttaaataaa attaaagtta acaaagacta ttaaagttag caaatattcc
    28321 tctacctaaa taattgcttt ttataaagag ttatttattt taattgaatt taattgaaat
    28381 acgattttaa ctagaacaat agattaatgt aatatttaat ataaaataat tataataatt
    28441 gaaaaggaag taatctatga tgaaaatagc agtcataaca accgaatttt taaaagagtt
    28501 tgtagataat tctataaaaa agttaaatat taatgcagaa attgaaatat atatctatag
    28561 agatttttct catgttggag atttatattt agaaatagaa gataggtttg atggatttgc
    28621 agttagtggc cccatcccca aaaaagctat tacaaaaaag gctggaacta ttaaaaaacc
    28681 tctagttgat taaaaaatag ctacgcacac tcgtgacttt agtcatgagt tagtagctaa
    28741 aatagtcagc atatagggaa acctatatgt agagatagga tagaacctat ccaatactac
    28801 ttgaattgct ggaaacccct aaagctaacc aaactaaaac ataaggatga aataaaccta
    28861 agtgtgaaag tcacgaaagt agaaaaaatt ggttagatag tataaggtta aatcctaagt
    28921 actttaaaat gggcaatcag caggtaagct ccgaatagga gaaacttcaa cgactattcc
    28981 tcttgaggga agtacactac aagctattgg tagtggaagt gggtagacct taacggataa
    29041 tgccgaagga taagatatag tctgtgcttg catgaaagtg taagaagttc ataagagaac
    29101 tgcataggta gtagcgaact tatgtgaacg acaacctcaa aaatgataaa agttaagact
    29161 tatggttctt atatatgtta tttaaaatta aatataaaaa accttcaaaa tacttgtttt
    29221 aagtaattgc atattttaat attataatag gtgatgaaaa atggaagtta ctcatggtag
    29281 aggatatgca tatagtatgt caaatataga catccagcat tatggggaga catctttgga
    29341 atcctagtta tttcgttgct actgtatcag aaaatacaga ggagcagata agaaaatata
    29401 ttaataatca gaaaaaacaa gtaaagtgag gtggtagtag tggaaaaagc ttataagttt
    29461 agaatgtatc caaataaaaa gcaacaagag ttaattaaca aaacttttgg atgttgtaga
    29521 tttgtatata ataagtatct tgctaaaaga atagatg
//
LOCUS       DAFKMX010000025        29360 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFKMX010000025 DAFKMX010000000
VERSION     DAFKMX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.025"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(97..966)
                     /locus_tag="LZB08_000969"
     CDS             complement(97..966)
                     /locus_tag="LZB08_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7314483.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1063..1920)
                     /locus_tag="LZB08_000970"
     CDS             complement(1063..1920)
                     /locus_tag="LZB08_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7314484.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2135..4153)
                     /locus_tag="LZB08_000971"
     CDS             complement(2135..4153)
                     /locus_tag="LZB08_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBM7314485.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKISIKEAA"
     gene            complement(4424..5200)
                     /locus_tag="LZB08_000972"
     CDS             complement(4424..5200)
                     /locus_tag="LZB08_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBM7314486.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5257..6228)
                     /gene="asrC"
                     /locus_tag="LZB08_000973"
     CDS             complement(5257..6228)
                     /gene="asrC"
                     /locus_tag="LZB08_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBM7314487.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6241..7032)
                     /gene="asrB"
                     /locus_tag="LZB08_000974"
     CDS             complement(6241..7032)
                     /gene="asrB"
                     /locus_tag="LZB08_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBM7314488.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7033..8040)
                     /gene="asrA"
                     /locus_tag="LZB08_000975"
     CDS             complement(7033..8040)
                     /gene="asrA"
                     /locus_tag="LZB08_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBM7314489.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8431..9120)
                     /locus_tag="LZB08_000976"
     CDS             complement(8431..9120)
                     /locus_tag="LZB08_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBM7314490.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9183..10031)
                     /locus_tag="LZB08_000977"
     CDS             complement(9183..10031)
                     /locus_tag="LZB08_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7314491.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10050..10937)
                     /locus_tag="LZB08_000978"
     CDS             complement(10050..10937)
                     /locus_tag="LZB08_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBM7314492.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEKNEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(10995..11798)
                     /locus_tag="LZB08_000979"
     CDS             complement(10995..11798)
                     /locus_tag="LZB08_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314493.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDKNWFLNDTIEKF
                     NAGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(11958..12410)
                     /locus_tag="LZB08_000980"
     CDS             complement(11958..12410)
                     /locus_tag="LZB08_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBM7314494.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DVFVNIVEYRTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(12540..14042)
                     /locus_tag="LZB08_000981"
     CDS             complement(12540..14042)
                     /locus_tag="LZB08_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBM7314495.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(14075..14941)
                     /locus_tag="LZB08_000982"
     CDS             complement(14075..14941)
                     /locus_tag="LZB08_000982"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBM7314496.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEGVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(15026..15697)
                     /locus_tag="LZB08_000983"
     CDS             complement(15026..15697)
                     /locus_tag="LZB08_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBM7314497.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(15970..16461)
                     /locus_tag="LZB08_000984"
     CDS             complement(15970..16461)
                     /locus_tag="LZB08_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBM7314498.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTHQKIREGSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNINDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(16483..17847)
                     /locus_tag="LZB08_000985"
     CDS             complement(16483..17847)
                     /locus_tag="LZB08_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBM7314499.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINLAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYFSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            18266..19441
                     /locus_tag="LZB08_000986"
     CDS             18266..19441
                     /locus_tag="LZB08_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7314500.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(19614..21011)
                     /gene="asnS"
                     /locus_tag="LZB08_000987"
     CDS             complement(19614..21011)
                     /gene="asnS"
                     /locus_tag="LZB08_000987"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBM7314501.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(21088..21321)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21490..21684)
                     /locus_tag="LZB08_000988"
     CDS             complement(21490..21684)
                     /locus_tag="LZB08_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBM7314502.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(21957..23630)
                     /locus_tag="LZB08_000989"
     CDS             complement(21957..23630)
                     /locus_tag="LZB08_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBM7314503.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(23640..27035)
                     /locus_tag="LZB08_000990"
     CDS             complement(23640..27035)
                     /locus_tag="LZB08_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBM7314504.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDMGYRSINKSFKDEENSYKLIDGYNIIH
                     NDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSINN
                     TDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINIT
                     GRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDINR
                     AIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNSA
                     MLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGNE
                     GNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKSV
                     GISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYIIT
                     GRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRGP
                     TIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYPN
                     QAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(27202..28398)
                     /locus_tag="LZB08_000991"
     CDS             complement(27202..28398)
                     /locus_tag="LZB08_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBM7314505.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(28419..29240)
                     /locus_tag="LZB08_000992"
     CDS             complement(28419..29240)
                     /locus_tag="LZB08_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBM7314506.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         9463 a         5270 c         2986 g        11641 t
ORIGIN      
        1 gctacatcaa tagtaagcaa ctattaatgt agcatgattt tttgtatcta aataatctta
       61 gtatttacct atcaatatag attgatatat caaaaactat agtcttctat attcttctct
      121 ttttagcttt tggttactta gatagtaatc aatttcatca attagacgtt gtttatcatc
      181 aggtaataca ccttttaaat tagcataatt tgatgcatga ttacttctaa atacaatttt
      241 ttcattagca tctatattct ttatcatctc tttaatttct tgtaacacag ccttatcctc
      301 tagtattgta aactctcctg ccttaagtaa atcgtaaagt tcagtattag gttctaccat
      361 taaactaagt acacccacat aatctggtga aattgctcct aacatttttc ctgtatcaac
      421 tgcatgctga tgtgtttttt tagttcctcc aagacctgct attacagtgg ctgataattt
      481 aatcccactt tttttaactt ttctaccaac ttttattaat tgttcactac taaaaccttt
      541 cttaatatct tctaatactt cattgcttcc actttcaagc cctagataaa tcattttaac
      601 accaagtttt cttaattctt taagttcatc atcagatttt ttctctatag atttaggaga
      661 accgtacata gtaattcttt cacactctgg aaaaacttct tttatatata aaataatatc
      721 tcttaatttc tccattggta ttatcaatgc atctccatct gctaagaata ttctttctac
      781 atgcttataa tatcttctaa atatatcaat ttcatttttt attacttcta gtggctttat
      841 tttaaatttt ttagatttgt acatactaca aaacgtacat ttgttatgag aacatcctag
      901 tgtgacttgt attataagac tataagcctc acttggtggt ctgtaaagtg gcatatcgta
      961 ttccatatta aaatccccct tgtaataaaa tattcattct attatattat taatatttac
     1021 tttaaataat aacttataaa attatatttt ttaaatttta gtttaagata tattctttaa
     1081 aaatttaatg atactattgg cagtaatatt aagttgtttt tcttgactta taatagcatc
     1141 attatctccc ttttgttttc catagtctcc aaattgtgca tgatttcctc cttcaatctc
     1201 aacataagtt gtatcatcag gaagtttttg ttttgattct acaagacttt taaaattgac
     1261 tactccatct ttgctccccc atatagatat aacttttttt cctaattctt tcaactcatc
     1321 acccattgga taagaagcta aaaatacaac gccttcaatt aatttattat cacgtgcaaa
     1381 atttgatgct gcaactcctc ctaatgaatg acctcctatt acccaatgtt ctatgttgtt
     1441 gtaagaatcc ataactttct gtgccttatt agttccaagt atagctagat taagtggcat
     1501 ctttacgatt acaacctcat atccactttc tgctattttt cttgcaagtg gagcataaga
     1561 ttctatttct actttagctc ctggatacaa aattaaacct ttagaaactt ttcttccttt
     1621 tggtgtaaat gaaatgtttt catttacagt tacttcaact ttactattgc tgactagagc
     1681 ttcttttgcc agcttttgag gttcatatgt tttatttatc catatgaaaa agccaagcaa
     1741 aatcaataca aatagtatac ctattccctt aaatatctta ttctttatgt taaattttct
     1801 attattattt ttatcatctt tgttctcaac ctcatcataa agtaaaggtc tctcaagttc
     1861 catagttata ctaaatgtat catatttctt attacgcttt acctttacat ctttactcat
     1921 aaatctaacc cccataatat ttgaatacta ttatagttta tcctaagtaa attgtttttt
     1981 caaccaatga aaaaaatcaa tattttaact attttcatat aaaattcata aatatatgtt
     2041 ttttgttttt tatatattat tttcttttag tattttataa aacaaatagc catatacttt
     2101 attttaaata tatggctatt ctataattta atatctaagc tgcttctttt attgaaattt
     2161 tagttatatt acttattcta tacatttctt tgacaccatt tagagtttgc agtaagttta
     2221 gacatattat cttgtcacct agacttttag tatttacaat actctctaaa attacacttc
     2281 ttccttcatc atacttatct acattgtaat ttgaaatata attagtatct ctcaatattt
     2341 tttcaacatc ttcatctttt attttatttt tattaacatt tatatataga ttatacatat
     2401 tacttattag taatttctga ttttctaaaa aatcctcttg atgtttatct ttatatatag
     2461 catttatcaa cataagtcta tcttcaataa gtgatggtat caataataaa ttttttatat
     2521 agtaaacatc agctacattt tctatagact tattgacttc atcaattata tttttaacta
     2581 ttcctttata catttctata acatgttgat agcctgtttt agcatcatat ggtaatataa
     2641 acttatttac tattagtgca ataattgctc ctaatgctac atatgaaatc ctgcttatgg
     2701 ttaaaacatt tgggtctccc ataactactg ctgaaccaag agctgaaaca gttacacata
     2761 ccattaattt tctataatca actacataag agtttatata ccctgctaaa agaactatta
     2821 acgaccttaa tgtactatct tttattattg aaaataatac aataaatatt acagctccta
     2881 taaatgtacc ttctattctt tttctagacc tttgtataca attttctgcg taaggttgtg
     2941 ttaatgaaaa gacagttaac attatccatc ttccatctct taaatggaaa taatccataa
     3001 taaaacctgc tacagctgta gctaaagcaa tctttactgc ataagaaaat cttatagaat
     3061 ttaaattaaa gtttttctta tatattgata gtcgtttaaa ttcatgaggt atctttgatt
     3121 ttctttcagc tttcttttcg tctcttgatt ttactttttt ataatctact aaaaactcat
     3181 ataaattttc taaaacacta taaatttctt ttaaatttat atttttagta tcctgagcaa
     3241 taacataatc tctaaatcct aatatctctt tatcagttat ggtatcttta ttttctaaac
     3301 acatcttaat atagtttatt tctttcgttg ctacaactaa aacatctgat ttactattta
     3361 tgctactttc ttctaaaata ttttttatat cttcatcttc aaattcttta ctatcctttt
     3421 tgtatctttc caggattaaa cttattcttt ctaatgaaaa taaaatattc attatgtcta
     3481 tacctctaat actgatataa aagtcatctt ttctattatc aaaaataacc attttcaaat
     3541 ttttagatat agaatttatt ctataattac agctatctat ttttctcata ctagatttaa
     3601 ttatagaact atcatcatta atagtatttt ttaaaagact aattttttct aataattcat
     3661 cacatataga tatcagttct ttatctccga catttttcat cctatttcta ttcataacaa
     3721 attgagcaat cattattata actgctccaa cagctaaagc cgacaatctc ataggaaatt
     3781 cactttttcc tacaggtata cttaccataa atatatacat tagaccaaag ggtacatata
     3841 ctgctctttt taagtcataa ctaaatacat accctattat aaataatgct ataaaattta
     3901 ataaaactcc taaatacata ttttgtactg caaaaaatga tagtattcct aagattatat
     3961 ttattgctaa atacttcagt aaatttttaa ttggagaaat agttaaatct ctttccaaga
     4021 gtgttagcat agctgttact atacctacac ctataagtgt atttttactt ccaaataaat
     4081 atataaatat agaaataaat gctattataa aaataaacaa ctttgttttt gatattacta
     4141 gtttctttgt catcatatcc cccctaaaaa tttcaacaag accaatttta tcatatttgt
     4201 tatcttattc aaaatgcgct tttttacggt aggactttaa ctttttttat atttagaact
     4261 atataattca tatataaacg tattttttat atatataact gatatatttt cgtatgctcc
     4321 catatttttt ttgtttcaaa tattttttca aaaatataaa aataactctc tcaacaatac
     4381 taaaaatgta tttatcaaga gagttataat ttatacaata aatttaatta agcttttctt
     4441 tacctaatat ataagttcca agacccataa ataaagctcc tccaacaata tttccaagtg
     4501 ttacagctac taaattataa atagctcctc caatagtaac tgtatttatt gttggtgaaa
     4561 ataatgaaac actataaatt gtcatatttg ctacactatg ctcaaagcct gatgttataa
     4621 aagcaaataa acataagaat atcataatta tttttgcagt atcactatta gttctaaacg
     4681 aacacaacac tgatacacat actagaatat tacataaaat ccctctaaag aaaagagctg
     4741 taaatggcat ggatgcttta ctagctgctg tatttgcaaa aaattcagct acaggtcctt
     4801 tgtctactag accagttcct acaaatataa tgcctaaaac caaagctccg attaaattac
     4861 ctacccaact gtatgcccat attttagaag tatcttttat acttacacct ttatttaaca
     4921 taccagcaga cataaccata ttgtttccag taaataactc tgttcccgtc attataacca
     4981 aacttagtgc tattgcaaat gataacccca ttactatctt agtcataggt gaacctgcat
     5041 cagtcaaaag tcctccaata gtaaatatca aaagtatacc tatacctaca taaagtcctg
     5101 caaacgcaga agataccaaa tattttactt tacttgtgtt taataaattt atcttattta
     5161 tagctgcatt agttaattta tccaaagttt ctttatgcat aattccctct tccttctatt
     5221 aaattttaat ttattcaaat atatgtttta ctcaatttat agatatttaa ttccactcca
     5281 gtatatgttt tcgtacattt tagttatttc tggaagctct acaccttcaa gtgcccattt
     5341 tttaaattcc ataaatcctg ttctatcaat tatatatcct atatgttctt ttcctcctgg
     5401 tgcatcttta tctatatact tttctacata tctataagta ttaagtatta tctttattat
     5461 gctgtcctca tctacccata aaagaaaatc ttctgctagt ctagggtttt tctttcctgt
     5521 tctacccatt atagctaatc tatagtattt tttctcatct ctagtccatg catttgttgg
     5581 gcaatttagt acacactctc cacagcctat acacttatct ttatctctta ccaccttatt
     5641 attttctgtt tttaatgctc caacagatat cttagaacat ttttttacac atgctccaca
     5701 atttacacat ctatcctttt catatattgg taaagccata ccaataattc caaagtcatt
     5761 cattctagct tttatacaat catttggaca accagttaaa gccactttaa aatgtaaatc
     5821 atttggaaat atagcttttt ctattctttt agcaaattca gttgtattat attgagcctt
     5881 tggacagact ttatttccta tacaagctgc tatatttctt gtacctgccg ctggataacc
     5941 tgcgtttttc tcactttgat ttatattcat tttttcaata acaggttgta taattttatt
     6001 tatttcttcc atatcttcca tatttattcc taatatttca aacccctgtc ttgtagttat
     6061 gtgcacctgt ccattgccgt actcacttgc aattttagaa actacagcta aactctcagg
     6121 gtcaataaga cctccaggta ctctaactct taaagaagtc tcatattttg tttttgtaat
     6181 tctataagca tttttcatta cttttttggt atttaaatct cttatcacaa acttcacccc
     6241 ctaatcaata agatttttag catatgaata atcaaatact ggtccatcta aacatatgta
     6301 agtatcatcc attttacaat gtccacattt acctattcca caacacattt ttctttcata
     6361 agaaacccaa atgttctttt catctaaatc tctttttaaa aattcttcta ctgtaaattt
     6421 catcatcatt ggaggtccaa ctacaactat agaagtattg tttatatctt tcatatccaa
     6481 ttctggtatg tattttgtta ctaatcctac attaccacta tatccttcct ctgctccatc
     6541 tacagtaact aaaatatcaa gttttttact ccatctcttt aaatcatctg caaataaaat
     6601 atcttttggt gatttaaatc ctactattaa tttaaacttt tcgcattttt ctttattatt
     6661 ataaaaatat tcaactattc ctcttacagg ggctaatgca cttcctcctg ccactactac
     6721 aatttctctt ccttcataaa gggatacatc aaatccattt ccataaggac ctcttataaa
     6781 aaaagaatct ccttctttat aattaaacaa ctcgctagtt acttttccta catttcttat
     6841 tgtaaagtca atataatcct ctccatatcc tgatactgat attggacttt caccgtattt
     6901 aggtatagat atctcataaa actttccagg caatacacct tttgtattgc agtttactct
     6961 aaaagtccat tctttatcag tatgtttaac tatatctagt atttttgcac ttactggtat
     7021 atatgggttc atttatactt cctccttact aattactaaa tcatttattt tttctataca
     7081 ctttgaaaat gatatgtatt gcggacaggc atcatcacat cttccacaac caacacacat
     7141 atgatatccg aatctttttt taaaatcatg tattttatgc atagttttaa atctcattct
     7201 ttcaccttgt ttttttctaa aagaatttcc acctgctata tctgtatatc catctacttg
     7261 acaagaagcc caaacccttc ttctctcacc tacattttcg ttttccttat agaaaatatc
     7321 ctgcattgta aaacatgaac atgttggaca tacaaaatta catctaccac atcctataca
     7381 tcttgtatca tattcatccc atattgaatt tcctatcatt tcatttgaat caatattctc
     7441 tggcacatct acagatatta aattatcagt tacataatca acattaaatt cagttgcttc
     7501 tccattaaat acttcaaatt cactatcctt tatatctaag tacagtgtat ctccttctat
     7561 atttaatcca attgaataat tatcagtttt gttagaatcc atgctcacac aaaaacagtt
     7621 tctaaaactt tccttacacc ctacaagtgc aaactttact ttatctctga tatttttata
     7681 aaaataatct ttttcttcac cattatttag atatatttca tcaagtcttt ttataccatt
     7741 tatgtcacaa gctcttaaga aaattaaaat tttcttgttg tctaaatcgc tttccttaaa
     7801 ctctttttca gtaaaataaa acaatacttg atttatggga agtacaactt cttttggtga
     7861 aaagtttgct ttcttagcaa attccatttc ttcaaaagcg tttacctcct tgtatcttat
     7921 aacatcagta tctgaaaaag tacctttaaa tgggattaaa actggtgcaa atatcttgta
     7981 gtcttttttt agataattca gaccttcatt gaacttagat gaatctaatt ttagtttcat
     8041 ttttatcacc tttcttatat tttagtttta cttacttgct atcttgttta aatcttaaca
     8101 ctttattttc tatgcttccg tgataattat cacagtttca aaaataataa aaaaaatatt
     8161 aatggagttt attaagttct gttaatattt ttttattatc tacattattc gatttcaaat
     8221 ctaatcactt aaatacaatc attaagtaca ataaaactac tatctactca attttttgaa
     8281 taaaaaagaa tcttatttgt ttttttatac aattcaatag ggtaaaataa aaattaataa
     8341 attagacaat aaaaaagaat ctataaatag attctcttaa taatattttt atttaattaa
     8401 aattctaata atactcttac ttaatcataa ttaattattt ttaaaatacc ttgataattc
     8461 agtttcttta acataaaaac ctttattgtc aattttaata agttcttctt tttgcaatac
     8521 ctttatcatt ctggaaacag tctccctttt acagccaagc atatcagcta aatatgtaac
     8581 tgtaatattt acatttagta aagtccattc tccacagcaa actccaaatt ctctacttaa
     8641 cctatataat tttgctgcaa gttttttatc tacttttatg gatatagaat ttttcaactg
     8701 tctatataaa cgtcttattc ttctttccat ataagaaata atatcctttg taagttgaaa
     8761 atcattttgc ataatttcta aaaattcttc aatactataa taagctacaa ttgaattttc
     8821 aaatgcttca cagccaacag tagagttttt agaagcgtca agtgtaatat catttatgac
     8881 ttccccacta tttaatatga aaattacttt tctctctcca ttttcactta tcttaaaaat
     8941 agataccttt ccttctaaaa gtatataaac actgtctaag ctatcttttt cataaaataa
     9001 aatttctttt ttttgtaatc tttttagttt aactttttct ttaagttgat tcttcgtttt
     9061 agtacttaaa ttattaaaaa ttattacttt atcaatatta taatctttta atttagacat
     9121 gtaatatctc tccattccta aatttttaag agagctctat aaaacattac aaatttctta
     9181 ttttattcat ttttaaacaa ttttatagca tttgtagttt taattttcct ttcaatagct
     9241 tcattagaaa gttcttttac tacctgtgta tatactaaat caataatgaa aaattgtgct
     9301 aatttagaag atattgaacc ggtttccagt actgtttctc ccgatacata agataagtgt
     9361 acatctgaaa tatcctttaa ttcagaattt ttattttcag taattgatat aattttagca
     9421 ttattggctc ttgcaagctt tacagttttt ataatctcct ctgtttcacc agaatgtgaa
     9481 attgcaatta ttaaatctcc ttcttccatt atggaagaca tcattatcat tgtatgacta
     9541 ctgtcaaaac ttacacaatt aagacctatt ctcataaatt tatagtttga atcctgtgct
     9601 attataccag aatatccaat acctatgaag taaactttct ttgcatttat tataagcctt
     9661 gcgcagtcat gtacatcctt actattgatt atctctactg tgttctctaa agtggttaca
     9721 tttgaactta aaagtttttt tgctgtatcc aaagctggct catcattttg tatattttta
     9781 tttataatat tttttttatt gatagttgat atttcttgtg ctaaagtaac tttaaaatcc
     9841 tgtaatccat taaaattcat tttttttaca aacctagtta tagttgcttc accaatattg
     9901 ctctctttag ctatttgtga tattggttta taaaatacat catctatatt ttcttttata
     9961 taagctatta ataatttatc tgattttgtg actttaaaag ttggtgattc caattgttct
    10021 aaaatactca ttaaaaaccc ttctattctt tatattttgt ttaaaaaatt atataatgct
    10081 ccaagcattc ctgcatcatt ttgtctcttt gcaaattcta tattagtgtt tttgtaaact
    10141 ctctctataa gaacctcctt taatgctttg tctatttttg gttttaggaa cttttcttgt
    10201 gccataatcc caccacctaa aattaccact tctggattta ttacataaca tatatttgcg
    10261 attccaactg ctaatgattt tatcatatta tcgagttctt ttaaacaatc ttcatcatta
    10321 ttctttgcta aatcaaaaat tatttttcca tttaagtcat tttcattcaa attttttatt
    10381 tttgctactt ttttaattag actgctagta gatgccaatt cttgaaaaga acttccattt
    10441 acattcatat atcctacttc accagcacta ttactaaacc catttatcag tttattattt
    10501 attataatac atccaccaat tccagtacct attgttaaac atatagaaga tgttgcatct
    10561 ttacctgctc caagccacat ttcaccaagt ccagcacaat taacatcatt ttcaacttca
    10621 cattttatat taaactcttt ttctacttct ttttttaatt gcatgccctt atagtttgga
    10681 attaattctt ctaatgcaaa tagtatttta ccttcaactg ggtcaaccat acctgcagtt
    10741 gatatacata taccacttat ttcatttttc tctatatatt tttttactat atcttttatt
    10801 ttggctaata ttccttctcc acctttttcc tttgcttcag tatcaagact tgatttatca
    10861 attataaatc catcttctct aataaaacca tattttattg aagtacctcc aacatctata
    10921 caaacgtaat ttttcataac atatctccat acaaattatt tttataaatt aacaactcta
    10981 tttaaaattt tatattacac aaatctttta cttatctttt ccaattcaca gatatatttc
    11041 cttgcttctg ataagtctat agtatcactt aatacacttt tttctttggt tatatgatac
    11101 ttataaatat ttttacataa gtcctctcct aacatattaa gaccttcttg agtgtaatga
    11161 acatcatcaa tagttaatcc tctgtcatga aactgcatag caattttgct tcctacacat
    11221 aatatatcac attcactaca agccgactcc tgtgctgctg caattcgtct tgttctaata
    11281 aaatattcat cttctccaag ccaataacca gttaaactta caaacatttt atcaatcata
    11341 cattcttctt taagcctata cgcaattttt tttagacttt ctttatagta caatacatca
    11401 ctaccatatt ttgcatcact ttcaccttga atccaaataa cgtatttatt ttctatatta
    11461 taatgtttac ttaaggtagt acagcctgca ttaaattttt ctatagtgtc atttagaaac
    11521 cagtttttat catgattcca gttttttatt cttgaaccat cttttgcatt gtgaataaaa
    11581 caaactttat ttccagtaag cttattccat tcatttgaaa aagcaggtgc tatagtgcca
    11641 tcaccttctg aagtctctag ctcacatctc atatttataa tttcatcagt actcaaatat
    11701 tcaaaacaat tttcatttgg aatgatgctc tcttttggat ttcccaatcc tctagcattg
    11761 ctttgtccta ttaataagaa tacatctata ttttccataa gttaactcct ttacaaaatt
    11821 ttaagctttt aattattttc aattttctca taaaaaacta aggctgaaaa tagtaatcta
    11881 actaaaattt acgaagtctc actaatcgct gaaagttaaa tcactatttt cagccctagt
    11941 ggttatataa attatcatta tctaaccaaa accttaaaaa tagctttttt gatattttct
    12001 ttttctaaaa cttttattcc tgtcatatga gcatcttctg gataacaaac taaaaaatca
    12061 ccttttctaa gttctacgta tccattattt ttaccatcaa gaggcaagaa atcgccctct
    12121 tcttcatata ttttttgttc taaattttct ataaaattta tatctattcg ttcacatcca
    12181 tcaagcatca aatgaacatc tatatacttc ttatgagctt cccagaatct atcttctttt
    12241 tcacaagttc tatactctac aatattcaca aaaacatcat caccatctat tgtataagta
    12301 cctttttcat aatctattaa tgaattatct ttagcatatt caaaacatct aagtaaatct
    12361 tcatgcaatg ttgaatatgt cttactatga tttatatttc caaatatcat aagcactatc
    12421 tccctatata attataaagt ctatttttat acttttatat acaaagtata ttatacaatc
    12481 ttatagagca actaaattat ttatctaatc actctataag acaatttatc atttaaatat
    12541 tatttttcat aataaacagt agtctcagct tttggtaaag atacttttgc tttaacttta
    12601 gcttctattg cactaactat tgtacctacc actacagaag tcgtgattgt tattagagaa
    12661 tatgcccaag aagttacttc tggcatacgg tattttaaat aaacaactaa aattgctgat
    12721 actatgaatc caacatatgc tcccatttta gttgcttttt tacaaaatgc accaagtaca
    12781 aatattcctg ctaaaacacc tagtactagc cccataaaac tattgaacca ttcatatgct
    12841 gactttattt ctccatttgc taatactatt gcaacagcta tagaaagtat acctacacct
    12901 aatgatatat attgtgctat cttagtttgt ttttccatac ttatatcttt tgttatgata
    12961 gtttgtatgt ctaaagtcca acttgtagca actgagttaa gccctgtcga taaagttgat
    13021 tgagatgctg catatatcgc tgcaagtagt attcctgtta tacctactgg taattcataa
    13081 gctatatatg atgcaaatac ttggtcttgt tgaactgttt gagctaaatc tgggttttga
    13141 gtatagtata taaataaaca agttccaatt agataaaata ctgttgcaag tcccattgat
    13201 aaaacaccat ttccatatgt cattttatta agttgcttta tatcagttgt tgtagtaaat
    13261 ctttgaacta tgtcttgact cgatacatat gatgaaaaag tattaagacc agctccaaca
    13321 aatattataa atgcacttgt agatagtata tttttatcaa acataacttc attacttgct
    13381 aagaacttgt gtccagttgt aagagtatcc attactgttc caaaaccacc atttatactt
    13441 gctattaaat agaacaatgt ccctgtaaca cctactatta ataccattcc ttgtataaaa
    13501 tcggtccaca agacagcctt taatccacct gtatatgagt aaattattgc tattacaccc
    13561 ataactatta tcagtacatt tacaggtatt cctgtaagac cagccagaac tattgatgga
    13621 agatacatta taatagacat acgtcctatt tgatatatta taaacatcaa agctccaagt
    13681 actctaagac ctttactttc gtatctcatt tctaagtatt catatgctgt atctatttct
    13741 aacctactat atagaggtaa gaaaaatttt attgtcaaag gtattgctat aaacatacct
    13801 aattgtgcaa accacaaaat ccaagtacca ccataagaat ttcctgctaa tgataaaaat
    13861 gatattggac ttaataatgt ggcaaatatt gatactgatg taacccacca cggaatagtt
    13921 ccatcacctt tgaagaactc ttttcccttc atttcctttt ttgaaaataa caatcctgca
    13981 aataaaactg ccaataagta aaccactagt attgcaaaat ctatccaagt aaatcctacc
    14041 atttgactaa tcctcccata ctaaaagtat tttattaaaa atactttctg tatatttctt
    14101 ttgctcttga tttcatttca tcagttgctt ctttcatagg ttgtctacag tatccagcct
    14161 caacaccttg ttcttcaagt agtaatttaa ttgtttgata taagccattt cctaatatat
    14221 ctgttattaa atcattagtc acatgttgta cttcaagtgc ttctgatatt ttttcattct
    14281 tagttaattc gaatatttgt ctagctctga caccgtttac attgaatgta gaaccaattg
    14341 ccccatctac acctaaaact gtagctggta acatcatttc atcaaatcct gcaaatatta
    14401 atttatttgg gaatgttttt ctcattctct ctaataagta gaaatctgct gctgtaaact
    14461 taacacctat tattttttca ttttcaaata attctccaaa ctggtctaaa ctcatatcaa
    14521 caccagttaa aaatggtata gagtatatta ttaatctatt atctacagaa tttattattg
    14581 tattataata gtgttttatt tcttcaaaat caaatttata gtaaaaaggt gtaacagcac
    14641 ttattgcatc atatcctaaa tcagttgtaa atttagcaag ttctacacct tcttttaaat
    14701 taactgaacc aacttgagct attaatttta tttcttcttt cacttcatct ttagctattt
    14761 caaatattct tttcttttca tcagttgaaa gcatgaagtt ttcccctgta cttcctccta
    14821 cataaagacc atctacttta caaacatcta tattgtgtct aattatttgt cttaaccctt
    14881 tttcatttat atttccttcc ttatcaaatg aaactaataa tgctgaataa attcccttca
    14941 tatctgttgt tctcatatct ttacctccaa ttttaatttt caaatatagt taaatataat
    15001 aattttttaa tttataaatt aatttttaat taatttcaat tgctttaaca aatttttctg
    15061 ttattaattg tggtcttgtt attgctgaac ctactactga tgaatgaaca cccaactcaa
    15121 agaccttttt taaatcttga ggagtattta cctttccttc agctattaca ggtatttcta
    15181 aatctttaac taatctttcc attaatacaa aatctggacc ttctaatgtt ttagtatatg
    15241 gagtatatcc agatagtgta gttgaaacgc aatctacacc gtactcttgt gctttgatag
    15301 cctcatcata atttgaaata tctgccatta ctagaacacc gttttctttt atatacttta
    15361 taagttcttt taagtcttca ttatttggtc taactctatt ggtagcatct aatgctatca
    15421 tttcacaccc tgttgttaaa agttcatcaa cttctttttt agttggcgtt atatatatat
    15481 ctgaatcttc atagtttctc tttataatac ctataacagg aagtccagta acttttttta
    15541 tttcaataat atcttcaact ccttgagctc taattccaac agctccacct tccatagcag
    15601 ccttagccat tctccccatt ataaaaggac tatgaagagg ttcattttct aaagcttggc
    15661 atgatactat taatcttccc tttaccttat ctagcatttt ttaaattacc tcgtttctga
    15721 aatttatttt cttgatttat gtattaataa taacatgaaa actttttctt tgcaatattt
    15781 tttcatattt ttttctcgca tctatatttt tactgattta ttttatatat tagcttgttt
    15841 tttacattta aaagaaattt tatttcattt ttttggtgtt tattttcatt ttatataact
    15901 actgttataa gtccatttaa ataatttcaa taaaaaaacc catgtatact atacataggt
    15961 ttcccaatat tattctaaat ccattataat ttttcttcca cagcatggac aaaattcagc
    16021 ctctgtatct atctcacttt cacaataacc acaaattata aaatcatcac attcattttc
    16081 caattcaaat tttttaccac attcagcaca atatttatca tttatatttc caacataacc
    16141 acactcacaa gttacattac tttctgccat ttccatttca ttaatttcca tatttttttc
    16201 ataaattaac ttatcaagtt ctagtaactc atcacagaat ttatcaaaac tattatcatt
    16261 aatagaacct tctctaattt tttgatgtgt cagtattccc atttccaaga atatctttgc
    16321 tttcttttct tgaatatcat caatctctct atttaatttt gatttttctt tatataattc
    16381 aactttaaat tttccactac caaacttttt cttaacctta gaaaatttat catctacttt
    16441 attattagta tttccttcca cagaaattcc ctccaatcat atttaatcag ctgaatttag
    16501 cttatatcct attaaagata tcacaaagct atatacaaat gaaattacaa tagcttgtaa
    16561 gactttaaat tgcattatta aagttgttcc ataattagtt gtattaaaga aattaatatt
    16621 actatctagt ataaatgtac taaataaagc caatattccc atcaaaaatg cataatatat
    16681 actaaatact attataggtc ttatactatc tgtattatac ttaaatctta gcttgtatcc
    16741 atttaataat aatattaaca tagacaatgc tcccattgca taaaacatta atttagtatc
    16801 tccaaataaa gatgaagaac ttaaagtaaa cattgatact gttgagttta ttatggttac
    16861 tgaaattcca tttgcaaaag cccacatata actagctatt tgtgggagta tagtaaataa
    16921 atttaatcca tttgaataac tactcatatc tagttcattt aagtaactac tatcagatat
    16981 cgttagtacc aatagtatta ctaatactaa aacataacct aatatgaatg ttcttataga
    17041 atttgaaaaa agagagaaat acatattttc cttttcataa ctttttttat aagtagttat
    17101 gtatgtacat ataaacccta atacgaatcc atttaaaaga acactaaaaa attcataact
    17161 atattctaat gcatatccat attctaacat actatgtgaa ctagttttta cattagtaaa
    17221 aatagataaa atagctaaaa ctaatccata aaaaattcca actcctagag aatttgctaa
    17281 tacagttttt gaatctctac atctttttct cataaaaatt aggtttgata ttaataatac
    17341 caaaataggg attataagta atatcaacaa acctatattg gcatttataa acccagaacc
    17401 catcatggta gacatagata cactaatttg acccaagttg agagctagga ttatatgtgc
    17461 tagatttatt aaataactaa tatcattaaa ttgaatagtt ataatagctt taaatatcaa
    17521 tgcaataata aacaatataa atatggttgt aaatgttgtt aaaaatacac ctcttttatt
    17581 tgcatgatat gaaagctctt ttattttaga tttcaagtct agttttgttt caagtggtct
    17641 taatgatgct atttcataaa gttcatttcc acaaaattta cagtaattat ccttagtatt
    17701 attttcttta ttgcaactag aacaaatttt ttcttcttta cttttaagag tgatatggct
    17761 attttttatt tttctagttg gttcacctgt ctttttggaa taattattat gtgaataatt
    17821 catattattt attttctttt cacccatttt ttccctccaa gttttactat tgtaaattat
    17881 caaatataac tatatcatat aaaacttaat tataccttaa ttggatatat ttatctattt
    17941 tatttgttta ttgctgttgt tttttaacag gtcttgatat ttaaatagtt tgttaatgcg
    18001 aactttatta tttatatttt attatgctta atttgaattt attctattct ttattttcag
    18061 tagtaaacta tctgtaaatc ttaattataa taaagtatta atcaataaag ttactcgaca
    18121 ttatctaata aattttatct atcacaattt tagattgtta cacacaatat attttgatat
    18181 aattaaatca taaacgaagt attccaaata tctaatgata gaaaggaata cttattttta
    18241 tgctttattt ttaatggagg cttatatgaa cttagaaaaa ataaaaaaat tttttggatt
    18301 tcctctagac aaaaaacaca aagacaatgt tgagaaagaa ctctgcaatt atctttatca
    18361 tacgggtaaa atcatttgca taataatagt aatatctcaa ataattatga tgatatcaat
    18421 ctcagctaga gaaggaggcc cttttgtcac accaagacgc caatggtatt tttatatgta
    18481 tgtaacacta ttttgtgtat cttctgtctt tatgtttgta ttttcttatc tttggaataa
    18541 aaaaaaggat tgctgtacgt tttatataaa tttatggata atttatgtga cttttttctg
    18601 cctatggggt tgtgtaatta ctctacttga ccagttaggt ggaaatgacc taagtgtatt
    18661 tagttatatt atgttagcag ctgcagcttt ctcagtatta aaatttggag aatcagtaat
    18721 agtttttgga ggatgctttt tgtttcttaa tttattctta cctgggattc aaactatacc
    18781 aggcaatgag tttagtaata ttgtcaacac attttttata acatcccttg ccatttttgt
    18841 ttctacaatg ctatatagaa atcgtgtatt tacttactat gataaacttt taattagtca
    18901 acaatatgaa gaaatatctc atataaataa tttacttaac gacaaagtta tgacagatga
    18961 ccttactaaa atgaacaacc gtagatacct tgaagatatt ctacaaaaaa aattaatcag
    19021 tcacgcaaat caagaaccta taactggaat gatgatagat atagattatt ttaaacaatt
    19081 taatgataat tatggacatc aaaagggaga ctattgtcta caaaatattg ccaaagtaat
    19141 tttagatttt acaagttctg aaaatgaaca tgctatacga tatggtggtg aggaattttt
    19201 tatatgtatt tttaactgta atgaggcaga tgctaaactt aaatcagaat gtttacgaaa
    19261 aattatagag tcctgcaatt tcgagcgtga tgatgttcct ttaaaatgtg taactgttag
    19321 tattggagtt tatacaaaga aaattggaaa gacaataaca ttaaatgagt ttataagtaa
    19381 ttgtgataag gctctatata ctgcaaaaga acaaggacgt aattgtgttg cattttatta
    19441 aaaggttaca tactattaca taaacctata agtattatat cgcaaaataa aagtaatcaa
    19501 attatatatt tatagtacaa taaaaataac ttgcatatct ctaaaataga taaatatgca
    19561 agtcatttta gtgtttatac ttaaagttat ataagtttta ttatatataa ttattaaaat
    19621 tcagcatttt taggagttct tgggaaaggt ataacgtctc ttatgttaga catacctgtc
    19681 atatacataa ttattctttc aaatccaaga ccaaatccag agtgagtagc agtaccaaac
    19741 tttctaagtt ctaaatacca ccaataatct tcttctctta atcccatttc attaattcta
    19801 tctaacaata catcttctct ttcttctctt tgagaacctc ctacgatttc tccaactcca
    19861 ggaactagta aatccattgc tctaacagtt ttaccatctt cattcattct catatagaaa
    19921 gcttttatat cttttggata atctgtaaca aacactggac aattatatat ttgctccgtt
    19981 atatatcttt catgctcagt ttgaaggtca catccccact caacaggata ctcaaattca
    20041 tgcccagatt caattaataa ttccactgct ttagtatacg ttattctaat aaactcagag
    20101 tttactatct tatttaacct ttctaacaat cctttatcta taaaactatt aaaaaattcc
    20161 atttcttcag gagcattttc taatacatag cttataacat attttatcat atcttctgca
    20221 agttccatgt tatcttcaag gtctgcaaaa actatttctg gctctatcat ccaaaattct
    20281 gaagcatgtc ttcctgtata agagttttca gctctaaatg taggtccaaa agtataaacg
    20341 tttctaaaag aaagtgccat tatctcggca tttaattgac cactaactgt taaattagcc
    20401 tcttttccaa agaaatcttg agtatagtct attttgccat cttcagtttt tggaatatca
    20461 tttaaatcca tagttgttat tctaaacatt tctcctgcac cttcacagtc actacctgtt
    20521 atgattggag aatgtgcata tacaaagttt ctttcttgga agaatttgtg tattgcatag
    20581 gcagctattg atcttactct aaatacagct gaaaaagtat tacttcttgg tcttaaatgt
    20641 gctatcgttc ttaaatattc aagcgtatgt ctcttctttt gtagtggata cgaactatct
    20701 gattcccctt ctacaactat gctttttgca tgtatttcga ctggttgttt agccccttct
    20761 gtagatacta actctccctc tacaagtata gatgagctta ttgctaattt tcctacctct
    20821 ttaaaatttt ctactttctc atcaaatact atctgcatat ttttaaagaa gcttccatca
    20881 tttagctcta taaatccaaa atttttagat actctagatg ttcttatcca tccagctact
    20941 tttacagtct tccctatgta ctgttctttt tctctgtaca attcttttac tgttataaat
    21001 tccttttgca ttttaaatcc tcctagtata attgttatta atttctttta aatataattt
    21061 caataaaaaa agcccttcat ccctatataa agggacgaaa gactattact ctcgcggtac
    21121 caccctagtt gtcataatga cctcttagtt aagcaatgct taaagcctat aacggtgcct
    21181 tccgtctaag tctacttact aaataagctt tcgattagaa actccaagat gttcttcaat
    21241 ataaacctcg tattgagctt tcaccatcct caactcgcta gaactacatt ttatatctac
    21301 tcttctcttc acagtattta tcttttatta tttctgctaa ttaacaaaat tataccaatt
    21361 tataaatttt tagtcaataa atttatataa ttgatactga ttgttattta aaatataata
    21421 attctacctt acataatgat aataaatact tatcttcatt acttctttat tacttcaatt
    21481 tccaaaattt tataatttac ttccattgtc aacccagtct tttgcttctt cgacaccctt
    21541 aattgaaggt attgaaactt tactatgagg ttttaatttt tcaacatcag cccatgctgc
    21601 tgtcgactca tttccaacta aaagtgaact tttaagtctt ttattatctt taccttttaa
    21661 tgctcttgcc tttttacttt ccaaaactat cacctctatt atttattttg taatagtttt
    21721 agcaatttta caaataaaat tcataagtct aaattataat tatttttaaa ttaacatcat
    21781 atcttttcaa tacttaattt atacctattt atatacaaaa tatttacttt gtgtattcaa
    21841 aatataaaac tgttttttaa ttttcatatt tattttttat gctatttttc tactctatct
    21901 aaagtatttg atacaattaa actaataatc aattataatt ttacatagtt tcttatctat
    21961 agagtatttg ctatctgttg aatcgtattt tttaaattta aaatcccata tccctgcgat
    22021 acatttggat atgtatatat ctcaagtttt gtagcaccca atattaaata tgtttttaaa
    22081 acttgtgtaa acaatgataa tctcggtaca ttatcctgtt tttctaagta ttccatcaat
    22141 aaggctaaaa cacctgtgac tatagaactg ctcactcctg ttcctgttcc tgtattatat
    22201 gttccatttt tatatgtgga tattatatct acacctgaag ctactatatc tggcttaatt
    22261 cctcttcctc taattggtcc tttcgaagaa ccaatccaca tactatcagt cttattgtta
    22321 aatgtaccaa ctgtaattac atcatcacta gcagcataca tagttatagt agctactgaa
    22381 tcagggtcta aaaaccttgt gtctggagct atcaaatttt tatttggcaa gtatatgtca
    22441 tattctccat ttattataag ttctgaagtc aatcttaaaa tccatactcc aggttttata
    22501 tctcttaatc ttatctccaa attttctttt ccagaggtaa tatatggata aataaatctc
    22561 attgcataag tagtattttc taagttaaat tttcctctat atatataaaa gtctggagaa
    22621 taccttatat catgactaac ttcacctgaa ggagatatta tctgtgctcc cactttatct
    22681 ggaccattcg tatttagtgt tatatcaaga gcatagtcat ctccatcttg tattattaca
    22741 tcttgaactt ctcccacact gctaaatctg ccagaataat gaatatctgt attcccttga
    22801 tttcctgcac cactcactac taccacacca gcactactca atatattaaa agtatctagt
    22861 attgaagtag ttgctacagc acttgactta actccaatag tcaaatttat tatcagtggt
    22921 ttattttctg ttcttgctat gttagtaaca tacgttatag ctgctaaaaa atctgaaaca
    22981 ctgtaattta tccttccagc ataataagta tctgtatatg attttaattt aactactatt
    23041 aagtctgatt ctgtagtaat acctctgtac tgagaattta tccttccatt tccaactaat
    23101 ataccagaaa ctaaagttcc agtacctata ttatcttgac tcaaactccc atcatttcta
    23161 tttatggcta tatttaactc acttcttgta aattcacttc caaaaataaa accttctgga
    23221 ggtggatttg tatttgcttc ttggtcccat aaatataaaa ctttactagt tccatcatca
    23281 tttataaaat caggatgcaa ataatctatc ccagaatcta taactgctaa taaaattcct
    23341 ctccctgtta tatcattata tggattttca taaatataat cagtttcagc tgctgttgta
    23401 atggtttctc cattatttac attattagtt atttcaatta gactgctcat tggttcagat
    23461 tcttcccacc aagcaacttg tattatatta tttaaaatag tttcatcaaa atctaaaggt
    23521 acatatatta ctgcaagctg actatttaaa accatatacc tatttattcc attttcttgt
    23581 agggcagatt ctatatcacc ttgataaatt atacaataag atttttccat aaatccctcc
    23641 tatcttaaaa catcaaacat tcctctaaca tccaagagcc catatccaga atttgtattt
    23701 ggatatactg tgtttgaatc ttttctagca ccagcttgca taaaagtttt tattttttgc
    23761 acatatgctt ggtttggata tctaccgtca acaaaagtat attgaaaata cattgcagca
    23821 gcaccagctg catgtgcact tgctgctgct gtaccagtta tagttgcata cgtgttccca
    23881 ggataagcag ctattatatt gacacctggt gctactatat ctggttttaa cctgtcttca
    23941 atagttggcc ctcttgatga actttgccat aaactaccat taatagtatt gtaggctcct
    24001 atggttatta aatcatcttg aatcgcagga taatttatag tgtaaaaagg gtctacctct
    24061 ctaaatctag ttccactttt taaaagttct ctatttggaa ggtataagtt atatcttcct
    24121 gttataatat aaactcctac taatcgaatc ttccatacac ctctttttgc attctttaaa
    24181 gtaacattag taaattgctg tccagaaaaa gttgttgggt atatatatgt tatagaatat
    24241 tcagtcccct ctaaatcaaa taagcctgtt actttattat aatttgaaat tccaacactt
    24301 ttactttctt caccagtggg agatactatt attacatcag ctttatctgg tctatttaac
    24361 caaagttcta atgataattc ttcctcgtct tcatttaact ctaattcaac ttcaacagaa
    24421 cctccaacat atggtattat tccagatgta tgtgtctgtg tattaccctc atttcctgct
    24481 cctgccgtta tacataaccc tctagtaaag aacgcttttt cactattact cctattggtt
    24541 aaacctgcta ggctactggt acctaaagac atatttataa ctaatggtct tccaagttca
    24601 aaagcttttt tatatgcata ctgagatgca gcaaacaaca ttgcactatt ataaaaatca
    24661 tctatcttgc cgagttttat tattattagc tcagaatctt ctgctatacc tgcatattca
    24721 ctatttacat ttccaagccc tgcacatatc ccactcaaca tagttccttg accaacttca
    24781 tcctgagata gactagggtc attttctgcg atagctcgat ttatatcttc tcgtgtatat
    24841 tctgtcccaa tataaaaacc atcaggagga gttccttctt ttgtttggtc ccataaatat
    24901 actatttttg aagtgccatc tggataaata aaatctgggt gtagatagtc tattcctgta
    24961 tctgcaattg atattagagt ccctcttcct gttatattaa tattaggatt atttttaaag
    25021 aaatttacgc ccatttcttc tgttgcaact acacctccaa aagtacctcg attaatttct
    25081 ccaagtaatg tcatctttgt agtcgataca gttcttatga ttgaaggaag ttgcaaaact
    25141 cgttgtatac tactatagtc tgtattattt atagatagta cacctaatga atcacttatc
    25201 ttaaaaaacc tttgactcat acctaaccca gctagttctt cttcaaattc aggagtatgg
    25261 actatatcta ttcctgatag tattcctgat tgtctacttg catttttact tatataaatt
    25321 tcattctcta agtcattatg tattatatta taaccatcta taagcttata actattttcc
    25381 tcatctttaa atgacttatt aatacttcta tatcccatat cctctgtttc taaatcttga
    25441 tttatattag atagagtgta caaatctata tctgataaat ttaaaaaacc aaatcctgat
    25501 gaattatttg gataagattg attacttaat ctccttgctc cctttagaag taatgccctt
    25561 aatttctgtg aatacaaaaa taaatcattt ccatttacaa ttccccattc cataaacaat
    25621 gaacaaaccc ctgtaacatg aggtgtagcc atgctagttc cagttaatgc tccacttgta
    25681 cctcctggta aaaaagatac tatatcttca cctggagcta ataaatcagg tttaaaaaca
    25741 cctaattgtg tatctccttc accagaaaat atagatacta tatctgttct tgaattaaaa
    25801 ctacctactg ttataactct acttgctgtt ccaggaactg ttacagtaag ctcttgagta
    25861 ggaatcagaa atctagtatt tctatttaga ccttcagaag ttggcaaata tatatttaca
    25921 ttaccagtta ctatatctat tggttcaaat acaattttcc ataacccagg agttatctgt
    25981 gtatttgaac ttaattgtag agtaactctt cttgtgagag aatacggtgc tatagggtaa
    26041 aaataaccag ttattctagt ttcacccaaa gtattcctta tctcacctga agttagagag
    26101 attgcttgtg tttgattatt tgatgggttt accaaatgta cactaaaatc atcaacaaaa
    26161 tctggccata tatttatgtt taatatacgc tctccttccc caactataaa ctcaacttct
    26221 tctgtaatat tattttgaag cctaatcctt ttatgacctc ctttatctgc attatttcca
    26281 gctgctacaa ctatattgtt tttccaaaat aaacacatat catctatata ttgttcaaat
    26341 aaagatgttc ctctatgtga accttcatta cttccataac ttatatttaa tgtaacaggc
    26401 attcttaatt ctaatgccct atctaaaata aatttaattg ccctcataaa ctctgtactt
    26461 ctcgaaaaaa tatcggtctg tatatttcca actctaacca ctattattct ggcatcactt
    26521 gcaatagttg cacaaatacc tgcaacatgt gtcccatgta agctagttgt tgaaataggt
    26581 atatacatac ttccatctat tgcattattt atgtcttcat tagtatataa tgttccttct
    26641 ctaaaacctt ctggtggatt tccttgaata gattggtccc aataatataa tatcttagac
    26701 cttccatcac tatctctaaa cacaggtaga gtataatcta ttcccgaatc aattatacca
    26761 attatagttc cttttccagt taaaccagtt ctatttttaa atcctgttat tcctgtactt
    26821 gaaaaacttt gcacatcctg agtctgtaat ataaaaggct tttctataaa ttcaatttct
    26881 ggataagtta aaagtatatt tacatcttct tcattacttg atgttattat tgcatatgat
    26941 gaattaagta tctctactga tacacctaat tcttcttcta acctcaatat atctccattg
    27001 tattttacaa ttaattcata atttattata atcacctcga cttctaaaat tattattaat
    27061 gtatatgttt tgtgtgagtc taaaatgtat tagactatat aattttttaa gtttcagtta
    27121 tacaatttat aataggtata ttaaaaaaat aaataatgat ggtacaattt tgaataaatc
    27181 ataccatcat ttatatttac tttatcttga aaccattaaa tttttcaatg ctttctcaag
    27241 accttttaca gtcaatggaa acatattaaa ttcactttgt atcattcttt gagtttgata
    27301 ccaataatta attgaatccc atccatctct aagttctgga ttcatccata caattttact
    27361 gtactttctc ttaaaccttt taagccactc tataccaggt gtgtaattat atggaccacg
    27421 atagttacca ccaatatgaa gcaactcaga aggcgacata gaagcatctc caacaattat
    27481 aactttatag tttttatcta tattttttaa tatccattca gtattaatag atttacttaa
    27541 ccaacattca ggtgttgtaa ataatctatc ataaatacag ttatggaaat aatatatctt
    27601 tacatcttta aaatgatttg atttacttac agactgaaat aatgtattac ataaagtgct
    27661 ataccctctc attgaaccac ctgaatccat aagcaataaa agcttcactg tattttttct
    27721 tggcttctca aaaaataact ttaaatatcc tgcattatta caagtctctt caatagtctt
    27781 atccaaatca agctctgtct ttggagcatc tattctagta gaaaattgac gaagtctctt
    27841 tagtgcaact tgaaattgac gcatatttaa aacttcatca tctctaaaat ctctgtattt
    27901 tctttctcct gcaacttcaa gaacagctct accataagta gaatttccac ctatacgtat
    27961 accagtttgc ccttttcctg aatgtcctaa ctctgaagtg cctcctgtgc ctatccaata
    28021 attaccacca ttatgaggag aatcttgttc ttttattcgt tcctccaatt tagctcttaa
    28081 tttatcaaga tttatatttg gattttgttc taatctttca aactcttcta aatctatatc
    28141 tggtttttta agccaattca taatctcttc tggaatttct tcttggtgtt ctataccttt
    28201 aaaatactcc aaaaacactg catcaaactt atcaaaatca ctttcacttt ttattaaaat
    28261 catcctgcaa agatagtaaa aatttgaaaa gtttgaatag cacagtcctt tatccaatgc
    28321 ttcaattaaa gtcatccatt catttaaaga tacatttaat cctcttgacc tcaataaata
    28381 aaaaaattca ataaacataa ttatccagct cctattaact ataagtgtct ttgcatagat
    28441 tcaatatcct cattattctt aagtagtatt cctgcaaaag gaacttcttt ttctattttt
    28501 tcaataggca ttccactcaa agttaatgct tgtatccagt ctattaactc tgatgtacta
    28561 ggcttttttt gtatgtcctt taaacttcta atccaataaa aagtagtcat tacttgttcc
    28621 aataggtgtt cttctacttt atcaaaatgt actttaacta tttcttccat catgtctcta
    28681 tcaggaaatt ctatataatg aaatatgcat cttcttaaaa aagcatctgg aagttctttt
    28741 tccgcatttg aagttataat aactattggt ctttgtttag ctcttacagt ttcttttgtc
    28801 tcatttatgt aaaactccat tttatccaat tcccaaagca agtcatttgg aaattctaaa
    28861 tccgccttat ctatttcatc tattaaaaga attacttgtt gatttgagct aaaggcctct
    28921 cctagtttac catattttat gtacttgcta atatcatcta caccttctcc tccaaattgg
    28981 ctatcataaa gtctttgaac agtatcatat acataaagcc cttcttgtgc ttttgtagtt
    29041 gattttatat tccaaattac taaatctttt tttaattcat ttgaaattgc ttgtgctagc
    29101 atagttttac ctgttccagg ttcgccctta atcaaaagtg gcttttcaag agcaatagca
    29161 acattaactg atgccatcaa ttctggtgat actacataat cactacttcc tctaaagtta
    29221 gatatctctt tttttatcat aaatcctact ccttttaaac agtattttct aaatgtatat
    29281 agactttatt actatttttg atataaaaat aataatacaa tacatttgta tagtattatt
    29341 ctaaaattct atatctaaat
//
LOCUS       DAFKMX010000026        28708 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFKMX010000026 DAFKMX010000000
VERSION     DAFKMX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.026"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(270..2549)
                     /locus_tag="LZB08_000993"
     CDS             complement(270..2549)
                     /locus_tag="LZB08_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7314507.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2820..4274)
                     /locus_tag="LZB08_000994"
     CDS             complement(2820..4274)
                     /locus_tag="LZB08_000994"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBM7314508.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4438..5307
                     /locus_tag="LZB08_000995"
     CDS             4438..5307
                     /locus_tag="LZB08_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7314509.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5520..7592)
                     /locus_tag="LZB08_000996"
     CDS             complement(5520..7592)
                     /locus_tag="LZB08_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7314510.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PVLANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNDLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(7895..8737)
                     /locus_tag="LZB08_000997"
     CDS             complement(7895..8737)
                     /locus_tag="LZB08_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7314511.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTKIIAYAEANLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8998..11040)
                     /locus_tag="LZB08_000998"
     CDS             complement(8998..11040)
                     /locus_tag="LZB08_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBM7314512.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLAVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11192..12163)
                     /locus_tag="LZB08_000999"
     CDS             complement(11192..12163)
                     /locus_tag="LZB08_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBM7314513.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12164..13141)
                     /locus_tag="LZB08_001000"
     CDS             complement(12164..13141)
                     /locus_tag="LZB08_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314514.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13360..14919
                     /locus_tag="LZB08_001001"
     CDS             13360..14919
                     /locus_tag="LZB08_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7314515.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            15025..15984
                     /locus_tag="LZB08_001002"
     CDS             15025..15984
                     /locus_tag="LZB08_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314516.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     SKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            15987..16799
                     /locus_tag="LZB08_001003"
     CDS             15987..16799
                     /locus_tag="LZB08_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314517.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(17015..17917)
                     /locus_tag="LZB08_001004"
     CDS             complement(17015..17917)
                     /locus_tag="LZB08_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7314518.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18272..19450
                     /locus_tag="LZB08_001005"
     CDS             18272..19450
                     /locus_tag="LZB08_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBM7314519.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            19459..20115
                     /locus_tag="LZB08_001006"
     CDS             19459..20115
                     /locus_tag="LZB08_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBM7314520.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADKIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20115..20780
                     /locus_tag="LZB08_001007"
     CDS             20115..20780
                     /locus_tag="LZB08_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBM7314521.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            20885..21661
                     /locus_tag="LZB08_001008"
     CDS             20885..21661
                     /locus_tag="LZB08_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBM7314522.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGVTGQACLLDGGYTAQ"
     gene            21723..23057
                     /locus_tag="LZB08_001009"
     CDS             21723..23057
                     /locus_tag="LZB08_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBM7314523.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGAGKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            23553..24545
                     /locus_tag="LZB08_001010"
     CDS             23553..24545
                     /locus_tag="LZB08_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBM7314524.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDN
                     NNSGSTDKPDNNKPESNKPEDNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIE
                     RAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGE
                     NIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(24937..28476)
                     /gene="nifJ"
                     /locus_tag="LZB08_001011"
     CDS             complement(24937..28476)
                     /gene="nifJ"
                     /locus_tag="LZB08_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBM7314525.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         9880 a         4573 c         3762 g        10493 t
ORIGIN      
        1 tttatttttt aaaatatgta taatacacct atagaaatta ctattattac tatctaaagt
       61 attttgctat aatcccatat actcttacct caaaaacatt caaatgtaaa aaataccaca
      121 tattaataaa ctttgcccaa taattcctaa aataatatcc taacaatgat ttatgttttt
      181 ataatagtat atttttacat aaaaaagtga acattgatta acaaagttca cttttttata
      241 aatttttatg ttataattca caattttatt taatttgttt cttttctatt taaactatat
      301 ctatctaata ttaatttttc atattcttct attggcattg gtctagcaaa taaaaatcct
      361 tgagccatat cacaacctat aaaccttaaa aactctgctt gctctacagt ttcaacacct
      421 tctgaaacaa cttttatatc aagttgcttt gccatttgaa caatacttga aactacaatt
      481 tgactctttt ttgcatttcc cactgttata aagaattgcc tatctaattt caatacatca
      541 attggcatat ctttaagcat atttaaagaa gaataaccag aaccaaaatc atccatagaa
      601 attttaaatc caacagattt caaagtttgc attattttaa ataatatttt tgtattgtca
      661 aatattgcac tttctgtcaa ttcaatctca attaaatttg gttcaatttc atattctttt
      721 gttattcttt caagctcaga aataaagcct ggattctcaa gatgtactct tgataagttt
      781 attgatatag gaaatgtggg gaaattttca ttttcccaac gtttaaaatt tttacaaact
      841 tgggtaaata caaacatatc caattctgtt ataaaaccat ttttctcaaa aataggaata
      901 aatacacctg gacttattag acctttttta ggatgttgcc aacgtacaag tgcttcagca
      961 cctacaattt tagtagttga taaatcaacc ttaggctgta aataaacttt aaattgttca
     1021 ttttttaaag catcatgcat ttcttgttca atatcacgtt cttctatgag agtttctttt
     1081 aaattttcag cataaatctc ataaactttt aaatattttc tttttactgt agtttttgca
     1141 atcatagctt tgtcaagaca ggtagaaata tttgtttctc cctcttcaac aaaatataca
     1201 cctatggatg gaattaaatt gagttctact cccaaacttg tttttatcat tgacaatttt
     1261 ttacgtataa gttcacatat ttctataatt gactctctat tatttttctt ttcaaatatt
     1321 atagcaaaat tgtcatttga aattcttgaa aatacagatt gctctttaaa tatattctta
     1381 attatctctg ctatttcctt taatactcta tctccttctt catacccaaa cctatcattt
     1441 actagcttaa acttatctat atcaaaataa attaaaacta gattcttttt ctcatgagag
     1501 gataaaaatt ttcccccttc aagattaaat ttatttgaat tgccaatacc tgtaacgtta
     1561 tctttaaaag caaattcttc caatttttcc ttactctttt tatgtataaa tagtatataa
     1621 attgttaatg ccgtaaagat tataatcaag actacacatg ctgataatgt aagttttata
     1681 atagcatatg atttctcaga tatagcagtt ttaggaactt cagaaaaaag ataccaatca
     1741 ttgattccta ttggggcata acttaaataa tatccttttc caaacatact ataacttaat
     1801 gaacctgatt taaagttttt tatatttgac ttcatattat tgatactaga ttttgatatt
     1861 gaatcacttc tgccaatgta agaaaataaa ttttctttag tagagttggc gtttttactg
     1921 cttggattta aaacaatatc accattactt ctagttatat aagaatatcc ttttccatta
     1981 aatgtaggta cagaaagtat tttttcatac aacttcgtac tatgtgtggc aaatataact
     2041 cctttaacct cattatcttt ataaattggt acagaatata cattaatatt actattggta
     2101 tttttatcat cttctattac atcttttaat tttcctgtta taaccacttc tccttgcatt
     2161 gattttaaga aataatctct tttagagaag tcttctatat aaccatctgt agtgtatgct
     2221 gtaccatttg gtaaaataac tcccattctt ttaaatgaac tattaatagc ttgtcttttt
     2281 agtatagaaa ggatattatc tacttcaaaa gactcttctc cttcaataaa tattgaaatt
     2341 gactctagta actttatatc tccattaatt tgttttttta acacattgac gctttgattt
     2401 gctatttctg aaagatattg attcgtttct tcacctaatg tcttattcaa ccttgaacca
     2461 taaatcaaac aggaaattgt aattactaag atacttatga cagcaattat ggacaaacca
     2521 tacctattat tttttgcatc acttaacatt atgcttcctg cctttctcga atctatgtat
     2581 gtataatcat tatcttatga caattattgt aaacatttta taataatact tatttttcca
     2641 tatttactgt aattatacaa aattataaac tttattacaa caataaatta attaatatag
     2701 atattagtgt ttatattatt gcattatcta ttttacaatt atttacatat aaaaaaatgt
     2761 aatatcaaag tgaagcttga tattacattt ttgttcttgt attttgttta atttttatgc
     2821 tacatcttac ttaaacactc atttgcaact tctttatcat caaaatgatg cttaacttta
     2881 cctataattt gataagtttc atgtccctta cctgttataa ttacaacatc atcctttttt
     2941 gccatactta tagcttctgc tatagcttgt tctctatcaa caacaacatg gtaattttct
     3001 tttttcttgt caattccttc aagtatatct tttattattg cctctggttc ttcagttctt
     3061 ggattatcag aagttataat acataaatca gaatattttt gtccaattgc tcccatcaaa
     3121 ggtctctttt cagtatctct atctccacca cacccaaaca cagaaattat ttttccttct
     3181 gcaaattgtt gagttgtctt tagtacattt tcaagtgcgt caggagtatg agcatagtct
     3241 acaattacgc ttataccttt atcatttgga actgtttcaa accttcctga tactccatta
     3301 gaaagtctaa ggccttcttt atatattggt ttaggtatgc caagtacata gcaagcagct
     3361 ataactgcta aagtattata tactgtaaac attcctggaa ctggtataaa tattacttct
     3421 tcatatgaag gtgttattaa tctataagat actccatccg aatctgattt tatatctcta
     3481 gccataaaat cagccttagt atcaacacca taagtgtaac aaggtacatt tatacctttt
     3541 atattttcat atatcttctt tccaccttca tcatctatgt ttattatatt tgccatagta
     3601 gtcttaaaaa ataatttttc ttttgctttt ctataatcct ctaaatcttt atgaaaatct
     3661 aaatggtctg gtgttaaatt tgtaaatatg cctagtttaa agtctgtttc atcaactcta
     3721 tttaatgcca atgaatgtga tgaaacttcc attgcacagt agtcacatcc attatccaac
     3781 atattattaa aatgatattg taaatctata ctttctggag ttgttgaatt ggaaacaatg
     3841 tctttttctc catcaaaaat ttttatagtt cctataagtc caactttatt cttatttaaa
     3901 gttaatatct catttagtat agtagttata ctagtttttc cattagtacc tgtaactcct
     3961 ataacgttaa atttttgaga tggatgattg taaaaattat cagctacttt tgccatatct
     4021 tttcttgtat cttttacctt tataaatgta catcctttta ttgctacatc ttcttcaaca
     4081 agaaatgcac ttgctccctt ttctatggca tcttttatat acttatgacc atctgacaca
     4141 aatcctttta tacatataaa taatgtacct tttgtaacct ttcttgaatc atattgtaca
     4201 ttattaatat ctatatttaa ttcacccttg acattgatta tatccaaccc ctgtataata
     4261 tcatttaatt tcataaatgc tttcctccct ttaaactctt tctgctaaaa ttacactaca
     4321 tataatatta tattaagaat tttttactat gtgaatggtt aaaaatgctt gaaacattgc
     4381 aaaaaatgct ctataatttt ctataatata actgaggaaa ggtcttctat aaaaactatg
     4441 gataataaaa tcgggtactt aaacgataac tttaaaatat ttaatattag agataaaaaa
     4501 gatatacaat ttgaatacca taatcatgat tttaataaga taattatatt tataaatgga
     4561 aatgtaactt attttgttga tggtattccc tataaactaa agccttggga tatactcttt
     4621 atcagcaata atgaaatcca taaaccagtt atagattcaa atgtctttta tgagagaatt
     4681 gcaatatgga ttagcccaga atttatgaaa aaaaatagtg atgctagtag tgacttagaa
     4741 aaatgtttta aaatagctac agagaataaa tgcaatagat tgcgtttaaa tatgcatgat
     4801 attgaatcta ttaagagttt tattgagcaa ataaatattt ccgaatccaa tactgaattt
     4861 ggaaatgaaa tattgagaaa tacattattt atacaactta tgatattctt aaacagaatt
     4921 ttcctaaaaa atgaaaatat ggaatcaaca gaagatgttt tttatgatga aactattaag
     4981 aaagtattaa actatataaa ttcaaatata gatgataagc tttcaataga tagcatatcg
     5041 tctgaatttt ttataagtaa gtattatctt atgagaaaat ttaaatctca tactggaacg
     5101 tctatacaca attatatagt tcaaaaaaga ttaattttgg caaagtcact aataaccaat
     5161 ggtcacctta tgagtgaagt ttgtaataaa tgtggattca acgattattc tagttttgta
     5221 agggcattta aaaaaaacta cggggtatct cctaaaaatt atataaacaa taaaaatcct
     5281 ttggatatta ctcttttaga tgaatagata atttatgtaa attttatttt aataaaactt
     5341 gaagtattag tgtatttatc ttactaatac ctcaagtttt ttaatgtttt aaaatatatc
     5401 aaaatataat gatatatatt tctattactt aaaatatata ttaaataaat tgcatgttaa
     5461 ctgaacctat ttatatattt attaaatccc acataacttt tagttacact atcatatagc
     5521 tatcctattt tttcaatatc atctcttaat atacttactc tattctcctc ttttgctgtg
     5581 gttactcctt ttttaatgct taatttctga ttttgttcta aattcgtaaa tactacagga
     5641 cttattgttg atagatcatt tttttcaata tattctaagt ccattttaac taatttttct
     5701 cccgctttta cttcatctcc tacattcaca aatacttcaa atccatttcc ttctaaattt
     5761 acagtttcta tccctaaatg tataagtatt tctactccac tttttttgga ttttattatt
     5821 attgcatgct tagttggaaa tataactcca atagttccat caactggtga ataaatcaat
     5881 ccatcactag atttaattgc aaatccatcc cccatcagtt tacttgaaaa tacagaatct
     5941 ggcacttctt ctatatctaa tatctctcca tttgttaatg aatcaatctt ttctactaaa
     6001 gcattttcca tatttgcaac ttccatattt tttttcactt tatttttatc aacattttta
     6061 gtaacagtac tttcaacctt tgtttttccc tctattatat cttttatatc agactttatt
     6121 gaatccgatt ttggtccaaa tatagcttga acattgttgc caataaccat aacacctgct
     6181 gctccaagtg atttcaattt ttctttatct actttattta tatcatttac aaccaatcta
     6241 agtcttgtta tacacgcatc taggctcttt aaattgtctt tatttcctaa agcaatcaaa
     6301 atctctgatg ctaattcatt cttagacatt tcaatgactt cagcgttagt ttctgactca
     6361 tcttctcttc ctggtgtctt taaattaaat tttcttatca taaatctaaa tacactatag
     6421 tataatactg caaaaccaat acctaccaag atagccaagt accatggagt tctattagga
     6481 agaacaccaa ataataagaa atctattaat cctcctgaaa atgtgaatcc aattttaact
     6541 cctaataatt gcataattgc gtatgataaa cctgaaaata aacaatgtat tgcaaataat
     6601 actggtgcaa caaataaaaa cgcaaactct attggctcgg ttattcctgt taaaaaagag
     6661 gttaatgctg ctgatgccat tatacctgct acaaattttt tcttttctgg ctttgcttca
     6721 tgcaccatag caagagctgc tgctggaaga ccaaacatca taaatggaaa tgcacctgtc
     6781 ataaaagtac ctgctgtgaa atttactcca tctttaagtt gagcaaagaa tatcttttgg
     6841 tcacccatta caagctcacc agctttattt acatattctc caaattgata ccaaaatgga
     6901 ttatggaaaa catgatgcaa accaaatggt attaatagtc tttccaaaga accttccata
     6961 aatgctgcta gtgtttgatt tgcttctata acattttttg agaaactaag taatccctct
     7021 tgtattggag gccaaataaa agacaatata attcctattg ctatagcaac taatgaagta
     7081 acgattggta caaatctttt acctgcaaaa aaacctagat actgaggtaa ttcaatttta
     7141 taaaactttt tataaagata agcagccact atccctataa taataccacc aaatacccct
     7201 gtttgaattg ttggtattcc aacaatattt gcatacatag aattgtctac catatcttct
     7261 gtaacacccg ccataacacc catagatgta ttgagaatta aaaaacctac tatggctgcc
     7321 aatgctgcaa caccttctcc ttcagttaaa ccaacagcta ccccaactgc aaatataagt
     7381 ggtaagttat taaatataat ttgacctgaa ctttccatta tacttattat aagttgaaat
     7441 gtaggattcg ccaaaactgg aaaatgagcc aacaatgcgt ctcctgctaa agcattactc
     7501 acgccaagta atatacctgc tgcaggaagt aatgcaactg gtagcattag agattttcct
     7561 actttttgta aaacaccaaa tacttttttc atttactttt ctctccttat tattttatat
     7621 ttttttaaat aaaaaagact taagtttata aaaagttaca ttagtatact taattctaaa
     7681 attaaactta tgttaatata aacaaaccta tattaaccta atttcagaat taaaaataaa
     7741 ctattataat ttttttataa acataagtct tgcctgcatt atcagtaaca atcttaatgt
     7801 tttaaaatta ttattttata ttaaatttta aataatatat taaaattgaa cataatatat
     7861 taaatctata cattctgtat taaactactc tttattataa attcagtaca gatataactc
     7921 ttttgatatg taatgttaaa taaagtagtt catcttgact tacatagtaa tcataattct
     7981 tttctacatg agtttttatt ttatatgcac aatcataatc cttttcatac tttactttaa
     8041 ttatatctat aatttcatta tttgtatcat tgattttttc tttttttact aatcgttttg
     8101 caaagaattt taaatgagtc agaagtctat cataatttat atcatcttct ttaaattcaa
     8161 ctctataata atatcttata atatttagta tgtcttttac aattttagta attaatagtg
     8221 attcctttga acttccctta tagtttgaat ttactatatg cattgctata aaaccagctt
     8281 catctactgg aaatttcaca tcaaattctt tatttatata atctacagcc catttcccaa
     8341 tctcatattc ttttttatgt attcttttta tctcatgtag taaatcattc tttatagtta
     8401 tgttttcatg atatctttta attgcaaatg caatatggtc agtaagtgct acataaatat
     8461 attcataaag cttagtattc aaattcgcct ctgcataagc aattatttta gtactaacct
     8521 caaaaaattt ctcatcaacc tggcttgcta gtttactaat tttatctaaa aaactttcat
     8581 cttgtattac aaaagttctt tcaattttac ttttatctat ttcttgacct ttcttttttt
     8641 gaaacccaat tccacatcct gttaatatta attctttttt tatattttca tctaaaacta
     8701 atacaacatt attattataa atttgttgaa taatcataat caatatctct cccaaaatac
     8761 aatttccctt attcataaaa aaatacctaa attaagatac aattcacctg tataccttaa
     8821 cctaggtatt gcctgcttac cagtaacaat ccagcttgtt aacgcttata tattgtattc
     8881 aatttatggt tttattctat tataaataaa tatatatgtc aagtaaatta ttgaaaacta
     8941 tttcctattt tttagtatta attttaacat caaaaatcaa attttattcc attatattta
     9001 tgattctatc atcatcattt catcatagag aatattttcc aataactctt tagccacttc
     9061 tcttgatatt tgcttagttt caactaattg ttgaacactg tctctttgac tttgtatcgc
     9121 cattactctg tagtattcta ctcttgcctc aaacttatca tcattttgaa tctttctagt
     9181 aatccaattc aaataacgat tatagaatct gattgctatt aaggcctcat gtctgttatc
     9241 ttcatcagta tttttattta taaatgatat agcagtttta gtagaaagtg catatgcatg
     9301 gtgaaactct ttgattattt ctttattctc agatactatt tttgtaattt tttcttttac
     9361 ttctttcttt acttttctat ttataatcca catgcttatt tttaatttta caattttaaa
     9421 tatagatttt tgaacagatt ttcttgattt tgtaatataa aatctataca atttaaaggc
     9481 tctatcagaa attttaccat cttctaatag tttcaaaaca gctaataatc cctgttggtc
     9541 tgcttctgca atcatcatac ttataagttt attatctgga gtatttagta ttcctttttc
     9601 tgagtctacc aactgttcat ttaaatcatg tataactggt tgtacttctt gtgctttttc
     9661 attactaagt tttaaaatgg cttctttcaa aattagttta tatgcctctt tatcagataa
     9721 taaaggttct gactcttgta cgttattttt taataatctt ggcagtaaaa ctgttgccaa
     9781 tactaaactt ataagaataa cttctgatga tataaataaa acagtatctc tcattggaaa
     9841 tacatcttta tcagccaaat aaaaaggtat agataaggca gttgctagtg tgaatgtacc
     9901 atgaacacca caagtagcaa ctattagtga gtatttacat ttagaaatac tctcttttac
     9961 aacttcttca tccttaaaac ctaaaaaacc tacaaaaaaa ttatttaaag gattcttttt
    10021 aggttttata aaagaattat gccataaata tacccatata aatcttataa caaataaaat
    10081 aagtacaatt aaagtagaaa taaaagtcaa ttccaatgcc atattttcat ttttactact
    10141 aagtcctgta tatatacttg gtaacaaaaa accaagcagt gtaaagacaa atccattcaa
    10201 tacatactcc aaaacatacc aagtattgtc tgatattatc cttaatttag tagttgtatt
    10261 ttgtaaatgc tctttttcaa ttccatgagc tatccctgcc attacaacag ctaggattcc
    10321 agatactcct atttcttctg ctacaaaata tacaaaaagt ggagtcatta tttgtataac
    10381 tattaccatt ggaatttctt ccaaattcca cttgtgtaaa ctaagtctta tttttataat
    10441 tatgtatcct aaaatacttc ctactattat tcctccaaaa gctgtaatca taaattgaat
    10501 ccctgcctct tctattgaaa aaactcctgt aactgtggca agtactgcaa ccttaaatgc
    10561 tacgactcca gcagcatcat ttaataaaga ttcaccttca agtatactca tcatattttt
    10621 aggaaaattt aatccttgtg taattgattt tactgctact aaatcagttg gagatattac
    10681 tgctgccaaa gcaaatgaaa cagctaatgg catcccagga atgagaaaat gtataaagat
    10741 tccacctgca aacacggtta ttaaaactaa accaaatgca agaagcaaaa ttggttttct
    10801 gagctctaat agctcttttc tcgatacatt ttgtccttca ctgaatagta atggagctat
    10861 gatacatatt acaaatattt ctggattaaa tgaaaatgag aatttaaatg gcaaaataga
    10921 caatataatc cctagtacaa tttgatatat tgcaagagga atattactcc actttttgct
    10981 aaatacagtt gaaatcgttg tacaaaccaa taatcccaac aatgttacta aaattgacat
    11041 aactacctct cttttcaata ataatattat agattaaact tatatatcaa ctaacttaat
    11101 attaacagca tgaaatccca tcttaatcaa aaaaattttc agtttattct attattctta
    11161 ttatatacat caaatttaat ttttgaaata tctatactag atgacatgca acaaaatgat
    11221 ttcttgatat ttcttttaaa actggaatct cttttttgca tatttcctta caataagggc
    11281 atcttgtatg aaacttacat ccacttggtg gatttgttgg agatggtatg tctcctttta
    11341 atataattct atttttctta attgtagggt cttgtattgg tactgcgctc ataagcgctt
    11401 ttgtatatgg atgttgagaa tgtaagtaaa gttctccttt agttgcaatt tctattatag
    11461 aacctagata cataacacaa actctatgtg caatatgatt tactacactc aaatcatgag
    11521 atataaataa atatgaaagt ttatatttat cctgtaaatc cataagtaaa tttataattt
    11581 gagattgaat agaaacatct agtgcagaaa caggttcatc acaaactata aattctggat
    11641 ttaatgctaa tgctcttgct atacctattc tctgtctttg acctccagaa aactcacctg
    11701 gttttctatt taaatggtac ttagaaagac cacaactttc tacaacattt aatatattat
    11761 cttttatttc attctttggc actaaattat gttctataac tgcctcggat attatttgtt
    11821 ccacatttaa tcttggattt aatgaactat atgggtcttg aaatatcatt tgcattttgg
    11881 gtcttaactt tctaagttct tccttctcta acttatatat atctacacca ttaaattcaa
    11941 ctttaccttc agttttttca attaatctaa gaatggttct tcctgcagtt gattttccac
    12001 atcccgattc tccaactata gccaaagttt ctcctttttt taaatcaaaa gaaattccat
    12061 ctactgcctt tacacattta atttccttat caaataatcc agatttaact tcaaagtact
    12121 ttttaagacc ttctactttt aataaactct cttctttttt caattatatc tcctcctttt
    12181 tatataacca acatgcaata ctatgaccac tattcaattc tattaaagga ggtatctgag
    12241 ttctacattt atcacatact ttttcacatc tatcactaaa ataacatgaa tctggcattc
    12301 caattggatt tggtacttgt cctggaatcg aatacaatct tttatctttg ttttttccaa
    12361 gagcaggctt tgattttaga agtccaatag tataaggatg ttttggattt ttaaataatt
    12421 caacaacctc tgccacttct ataattttac cagcatacat cactaatact ttgtcacaca
    12481 tttctgcaac tactcccaaa tcatgtgtaa tcatcattat agatgtgttt aatttttctt
    12541 ttatattttt cattatatct aaaatctgag cttgaatagt aacatctaaa gctgttgttg
    12601 gttcatcagc tataagtaat tttggattac atgataaagc catagctatc attattcgtt
    12661 gtctcatacc acctgataat tcatgtggat atgaattata tatttcttca gctcttggta
    12721 tttctaccag ttttatcata tcgattacct ttttcttagc ttcttccttt gataaatctt
    12781 gatgtatcat aataccttca atgatttgac ttccaacagt aaacgctggg tttaaagatg
    12841 tcattggttc ttgaaaaatc attgatatct cattccctct tattcccata agttcatctt
    12901 cattcatctt taaaatgtct ttgcctcgaa agattatttc tccaccttct atttttgatg
    12961 ttggaagtaa cttcatcaaa gacatggctg ttacagactt accacatcct gattctccaa
    13021 ctattccaat agtttcacct tcatttatat taaaacttac ttcatttaca ctctttacaa
    13081 tccctttatc tgtatgaaaa taaactttta aatctttaac ctcaattagt gctttactca
    13141 ttattttctc ctctcagcaa aattacttaa gataacatac accttttgat tgtattttat
    13201 aaaaatttaa attacaacat tttcttacat tttattattt ttttctatta attataataa
    13261 tataatagtt aatttctata tatattgatt taataatgat tactattata caataaaaaa
    13321 tatgtgttaa atattatttt atggaggttt tataagaata tgaaatttaa aaaattggca
    13381 tcattaatct tagtttcatc attaatgctt acatttacag cctgtgcttc caatgataaa
    13441 gacaagtcta gtggaagcaa atctggtgga actgttgtaa tacctatggc aagtgaccca
    13501 gatataatca atggagcttt tgcaaacgtt aaagaagact ctcttgcatc aaatatagtt
    13561 tatgctcctc tttatactta tgaaaaagga aatttagtaa attatttagc agaaaaagta
    13621 gattttaaag atagtaagga attaactata aaattaaaat ccaatttaaa atggcatgat
    13681 ggaaagccaa taactgctga agatgtctta tttacattta atacagtttt agacgaaaaa
    13741 caaaattcac catcaagaca atacttattg gtgggtgaga aaccagttaa agtagaaaaa
    13801 attgatgatt taacagttaa gattacttta ccaactgcaa gtgaatcttt cttatatggt
    13861 atatctaaaa tttctccaat accaaaacat gtgtttgaag gagaatcaaa tatagctaag
    13921 tcagaaaaaa ataataatcc agttggttct ggtgcattca agtttaagga atggaaaaaa
    13981 ggtgaaagca tagtatttga aaagaatgca gattattttg gtggagaacc taaggcagat
    14041 tctatagcgc taaaaataat tcctaatgaa gcatctcaag aagcagcttt aaataatggc
    14101 gaaatttcac ttatgaagac atctgctgaa ggatatgaaa aggcaaaatc aaactcaaac
    14161 ttacaaactt atacttatag tgaagaaaga ttaaactaca ttgtatttaa ccaaaatata
    14221 tcaaacatgg ctaacaaaga agtaagacaa gccttatcat atgccttaaa tagaaatgaa
    14281 atgatagaat cagcatatgg aaaagaaggt tctgtacctg ctaaatctat attagttcca
    14341 gaagcagact tctatacaga agaaggtgtt gaaggatatg accaagatac aaataaggct
    14401 aaagacttat tggataaaag tggtgttaaa attgataaat taaaaatagg ttataacaca
    14461 ggtagatttg ggcataaaaa ctatgcctta gtagctcaac aagagttgaa aaaaattggt
    14521 atagaagctg aaatagttcc ttatgaaagt aaggcattct tcaatatact atttagtaat
    14581 agcactgaat gtgatatgta tgtaaatggt tatgcttggg gattagaacc aaacccatat
    14641 agaggtatgt ttgaaactgg tcaatattgc aaccaaacta aatattctaa tgctgaaata
    14701 gatgctttat gggaaaaagg atttactgag ttaaacaagg aaaaacgtga agaaatatat
    14761 aagcaaatac aacaggatat atctaaggat gcgcctattt acactataga ttatgaacaa
    14821 aatttaatgg cagctcaaaa aaatctaaaa ggaataaaag atgctaaacc atctcctgca
    14881 atattatttg aagattggtc taaactgtat gtagaataat tgatgtaaaa ttttatatta
    14941 ttaattagaa aaggagatgt ctaaaaataa tattgtttta ttattttaaa acgagacatc
    15001 tcttttaatt aggagaattt acttatgtta aaattaatat tgaatagagt aaaagtttta
    15061 atacctatgt taatcttaat atctatatta tcatttggac ttttagaatt agctcctggt
    15121 gacccagcag atgcatatat aaatccttta atgtcttcac aggatattga aaatataagg
    15181 gttaatatgg ggcttgataa accagtatat ataagatatt taaactggtt gaaaaacact
    15241 ttaagtggaa acttaggtat ttcttatata aaccatatgc ccgttacaga acaaattatg
    15301 gaaaaaatgg gaaatacatt tattcttatg ggtacttctt tgatattttc aatattggta
    15361 gctatacctc ttggaatatt tttagctgta aataaaaatt caataactag taaagtatca
    15421 tctgttttta attatatagg tgtttctata ccttcttttt ggataggtat gatactcatt
    15481 tcaatttttt cagttaaact taacattttc ccatcaggag gtatgcatac tattggaaat
    15541 gatagtattg gagatttagt aaaacattta gttttgcctg taataacttt aggtctatat
    15601 aatactgcta tttttacaaa ttatgtagaa gctagtgtaa atgaacaatt aaaaaaacaa
    15661 tatgttatta cagcaagatc aaaaggatta tcagaaaaag tcatactatt taaacatgtc
    15721 ttgaaaaatt cactgacatc attggttaca atcctaggca tgagtataca aaaattagtc
    15781 actggtgcat ttgttacaga agtagttttc tcttggccag gtatgggaag attaatgatt
    15841 gattcaattt tttcaagaga ttatactgta attatggcaa taactatgtt atcagcacta
    15901 tttttaattt taggtaattt agttgctgat atactatact tattaattga ccctaaaata
    15961 aaatctagca aaggaggatt ctagttgtgt ttacaagtat cttaaaaaaa ttaaaaaaag
    16021 ataaatcctt tgcttttgca tgtattactc ttagcataat aatcatatta attataactt
    16081 cttttatatt aaatatagac tctaatgcaa ttgatactac ttctaaactt caagaaccta
    16141 gcttaaagca tatctttggt acagacgaac ttggtagaga ttacttctct agagctcttt
    16201 atggtggaag gatttcactt agtgttggta ttttatcaat gcttatttct gtagtatttg
    16261 gtactgctgt aggtgttatt agtggatata ttggaggtaa gctagaccaa ttcttgatga
    16321 gattagtgga tatgcttatg agtataccaa gtttcttagt tttgattgta ttaaatactt
    16381 atctaacacc taaagtttcc actattatac taattattgg attcttaagt tggatggaag
    16441 tttcaagaat tgtacgtggc gaaacattaa aaataaagga aaatgaatac tgtctagctg
    16501 ctaaagctac tggtatcaaa actagaaata tactattaag acatatagtt ttaaacttaa
    16561 aagagactat tgtagttgct ggttcgttaa atattgctaa tgctatctta actgaatctg
    16621 ctcttagttt tttaggtctt ggaatccaat tgcctcttgc atcatggggt aatatgcttc
    16681 aagatgctca aagacatatt tttgacaagt tatatttagc tgtatttcca ggattactta
    16741 tatttttaac agtcatgagt ttgactataa taagcaaaaa attttctagt aatacttaac
    16801 aaattttata tagaaaataa aaacagtgat tatctaaaga tatacactgt ttttattttt
    16861 cgtttaaaaa aatacatatt ttgtatatta taactttaaa tactactcta ccatgttatg
    16921 taaagtgtat tattgctctc aaacatatag atgaaaaact aatagtttat tataataaaa
    16981 attgatttat ttatttgtta ttttaataac atttttaaga gttcttttta ctaaagaaat
    17041 ctataataaa tttaaataca tgttggtcta catggctata acgttctttt ttaggtctac
    17101 ataatataac tttccatgta ataggatgat taaaaggctt ctctataatt ttgctatttt
    17161 caaaaaaatc atgaactggt gatggtagaa ttgttataaa atcagaattt gtggttgata
    17221 gcaacaaaaa atcccatgaa ccagacatta tatatctttt taacttaaca ttttcatcag
    17281 taaatttttg cattagctta tgatgtatca taaaagtagg attaaatatt gcaagtaact
    17341 gctcattcaa atcgttccaa tctatcttat ccttcttagc aagtggatga tttatattta
    17401 aaaatgctgt aagttcatct tcttgaagca catgttcagt tactacactt ttatcaattt
    17461 tatgtggatg taacaatact gcataatcta attcctgtaa gataagcatt ctatttaagt
    17521 catatgcacc cttctctact atatcaaatt ctatatctgg attatttgca actaactgtg
    17581 caactacatc tgaaaatgct attccaagta taagtggagg tattcctatt ctaacttttc
    17641 ctttaaattg aactgaatct tctcttaaat cttccatcat atttttgtat tcatttatga
    17701 tatgttctgc atttacataa aatctttctc ctgctggtgt taacccattt aacctgcctt
    17761 tatacctttc aaataaatat acatcttcac tttcttcaaa attttttata atttgactta
    17821 atgctggttg agatatgtga agtgtcttag atgctattga taaattaaaa ccagatttca
    17881 ctatttcaac aaaatatttc aactgtttta tatccatttt aaaccccctt ccccaaaagt
    17941 ataatttaga ttatattttt tcttctaatg atattttata ggaaaatcaa cataatttca
    18001 ttaatatttt aacaaaatat ttttctaaat ctactataaa tttttatcct ataaattttt
    18061 cttatacact atataaaaaa ttagacttag acaaaacgct tttttcgccg taatctataa
    18121 ttagaaacag gaaaacctaa agcattagga ataaaagtaa ttgaaatact taaaaacaag
    18181 cttgtattaa aaaagtactt tattatagtg ttccctaatt tctttattca gaaaattttt
    18241 aattatcatt caagaaaggt aactattaga tatgaaagat gtagtaatcg tatcagcagt
    18301 cagaacacct ataggaagtt ttggaggtgt atttaaaaat acttcagctg ttcaacttgg
    18361 aactatcgct gttaaagaag cgatatcacg tgtaagactt aatttatctg aaatagatga
    18421 agtaataatt ggaaatgttc ttcaaacagg gcttggacag aatgttgcta gacaaattgc
    18481 tataaatgca ggtataccaa attctgttcc aagctacaca gtaaataaat tatgtggttc
    18541 agggcttaaa agtgtccagc tagctgccca atcaatcaca tctggtgaaa atgatgtggt
    18601 aattgcaggt ggtactgaaa atatgagcca agctccttat atcgttccaa ctgctcgctt
    18661 tggtagtaaa atgggtgaca taaccatggt agatagtatg ctaacagatg gccttattga
    18721 tgctttcaat cagtatcata tgggaataac tgcagaaaat attgcaacta aatttgaatt
    18781 tactagagaa atgcaagata aattggcatt agaaagtcaa aataaggctg agaatgctat
    18841 aaaaaacaac cgctttaaag aagaaatcgt tccagtagat gttttaatac gtcgaggaaa
    18901 aatagaaact attgataaag atgaatatcc taaattaggt atgacatttg aaggtttagc
    18961 taaacttaaa cctgcattca aaaaagatgg cactgtaaca gctggaaatg cctcaggtat
    19021 taatgatggt gcagctatgt taatactaat gagccaacaa aaagcagacg aactaggtat
    19081 tagaccactt gctaaaataa aatcctatgc ttcagctggt gttgaacctg aagtcatggg
    19141 tactggtcca atccctgcaa ctagaaaagc actgaaaaaa gctggactta gcatcaatga
    19201 tatagactta atagaagcaa acgaagcgtt tgctgcccaa gcattagctg ttaaaaatga
    19261 acttcaaata gattcatcaa agttaaatgt taatggtggt gcaatagcat taggacatcc
    19321 aataggagca agtggagcta gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa
    19381 agttgaaact ggacttgcaa cactttgtat tggtggtggt caaggaatat caatggtggt
    19441 ttcacgatag gaggaaatat gaataaaata gttagcattg atgaagctct atctcatgta
    19501 aaagatggag atacaataat ggttggtgga ttcatggcca atggttcacc agaaaattta
    19561 attgattatt tatgtagtaa aaatataaaa gatttgacac taatttgcaa tgatactggc
    19621 tttattgaca aaggagttgg caaaatggtt gtaaataagc aatttaaaaa gataattgct
    19681 tctcatgtag gattaaataa agaaactgga agacaaatga acaacaatga aacagatgtt
    19741 gagttagttc ctcagggaac tcttgctgaa caaatacgtg cagctggtta tggtctagga
    19801 ggtatactca caccgactgg tattggtact cttgttgagg agggaaagca aaaaattgtt
    19861 gtcgatagca aagaatacct tcttgaaaaa ccaatttatg cagatgttgc tcttctattt
    19921 gcatcaaagg tcgataaagc aggtaatcta gtttataaag gctctatgaa caattttaat
    19981 aatttaatgg catctgctgc caaaattaca atagttgaag cagataaaat cgttgaaatt
    20041 ggtgatattg acccaaatga agttaataca ccaggtattt ttgtgaatta catcgttgaa
    20101 ggaggtaatt tataatggat aaattagaaa tgcaagaata tattgcaaat agagtatcta
    20161 aagaattaaa agacggagcc gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa
    20221 attatattcc ggataatgta aacgttatct tacaatctga aaatggcttt ttaggtttag
    20281 gttcagcaga agatggaaat tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag
    20341 ttactatact tcctggagga tgtttctttg atagtgctac ttcttttgga ataatcagag
    20401 gaggtcatgt tgatataact gttcttggcg cacttcaggt tgacaaatat ggcaatatag
    20461 caaactatat gattccaggt aagatggttc caggtatggg tggagctatg gacctagtaa
    20521 ctggagctaa aaaagttatt gtagcaatgg aacatacttc aaaaggttct gcaaaaatat
    20581 taaataattg tactcttcca cttactgcaa caaatgctgt agatttgata gttactgaaa
    20641 tgggagttat ggaagtaaca tcagatggaa ttttacttaa agaaataaat ccagctttca
    20701 cattagatga tgttatttct gctactgaag ctcctttaat attgtcagat tcattaaatg
    20761 gaaaaatatc tgttgtttaa aataattaaa actagaaaat aaaaatctct tagacgtatt
    20821 acatataaat ttgtattgct taaaatatta atataaaaat aaaataaaat tggaggaaat
    20881 gaaaatggtt aaagataaag ttgtattcgt aacaggagct gcaagtggaa taggtaaaca
    20941 aatagctgaa tcgttcttaa aaaatggtgc taaagtaatg ttctctgata taaatcaaga
    21001 agctttaaat aataccataa cagagttaca aaaaaaagga tatgattgca tgagtgtaaa
    21061 gtgtgatgta actaaggaag aagaaattaa ccacgctata gacaaaactg ttgaaaagta
    21121 tggcagatta gatatattaa taaacaatgc tggacttcaa catgtttcta tgatagaaga
    21181 tttcccaact gataaatttg aatttatgat aaaaataatg ctgacagcac cttttatagc
    21241 tactaaaaaa gcatttccta taatgaaaaa acaaggcttt ggtcgtataa taaatatggc
    21301 ctcaatcaat ggtgtaatag gatttgctgg aaaggctgct tataactcag caaaacatgg
    21361 acttattgga ctaacaaaag ttgctgctct tgaagctgcc gattctggta taacagttaa
    21421 tgctttatgc cctggatatg tagatactcc acttgtcaga ggtcagttca atgacctttc
    21481 aaaaactcgt aatataccat tggaaaatgt cttagaggaa gttttatacc cacttgtacc
    21541 acaaaaacgc ctattagatg ttcaagaaat tgccgactat gctatgttct tagcaagtga
    21601 taatgctaag ggtgttacag gacaagcttg cttactagat ggaggatata ctgcacaata
    21661 gttattatat ttagggcttg aagaaagtat tcttcaggtc cttctactaa ggagggatat
    21721 ttatgagtat tatcgcgcca attggtataa ttattggtat catagctatt atatattttt
    21781 cagttaaaga aattcatata actatagctg ctccaatagc aactttaata gttgttattt
    21841 taaataaaat ggatgttgta acgtcaatgc taggtgctgg caaaaacaat tatatgggtg
    21901 cacttggaaa ctatataatg agttattttg caatattctt attaggctca atacttgcta
    21961 aatttatgga agaaagtgga gctactgtat caatagctga ttttatttta aataaatttg
    22021 gctctaatca tccttataga gttttagttg ctatatttat agtaagtttt atattaacgt
    22081 atggtggtat aagcttattt gtagttatgt ttgcggtcat tccacttgca agaactctat
    22141 ttaaaaaact tgatatttct tggaacctta ttcagatacc actttggctt ggtattgcaa
    22201 caattactat gactataatt cctggaacac cagccattca aaatgttata cctattcaat
    22261 atctaaatac gtctcttact gcagctacta taccaagtat tgtaggtagt ataggttgtg
    22321 ctacgtttgg acttgtctat atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa
    22381 cttatgcaac ttatacttca gaattagaag aagaatcttt agatagagaa ttaccaggat
    22441 tttttgcaag tatttttcct ctattagcac tcgtaattat agctataact ggaagtactt
    22501 ttggttcaga atttatgaaa aaaaatgtaa tttacatagc tttaatagtt gcaattcttt
    22561 tatctgcttt tttattccat aaatttctat catctaagat acagacattg agcataggtg
    22621 ctagtggttc tataggtcct atatttgcta catcttctgc agttgcattt ggagcagtaa
    22681 taatggctgc acctggtttt gatgtattta gcaaacttat aatgtcaata ccaggtagtc
    22741 cacttataag tcttactgtt ttgacttcaa caatgtcagc aataacaggg tcctcttctg
    22801 gtgcattagg aatagtaatg ccaaactttg cagattatta tttaagtact ggtctaaatc
    22861 cagaattaat tcaccgtgtt gctacaatag cttcaaatat actaactata gttccacaaa
    22921 gtggtgtttt tcttacattc ttaagcttat ctaagttaac acataaaact ggatttaaag
    22981 aaactttcat tgtagttgca gttggttcac taatagcaga aattattgta attacattag
    23041 gattactaat gtattaatat aaaacaaatt aaaattcata atatgaaagg tggtgtctca
    23101 aagtgaactt tttacttaat gagacactct ttttatatga aataacatat gcctatcatt
    23161 taaacaataa aaaaggcctt atctctattt tgagacagac cctttttatt tcttaaagat
    23221 ttaaataatt ataatttgtt gctaatacga aagcatctac tgaataaatt taaccaaata
    23281 aaaaaaaatt tatcataact ataaatttac gttatggaat tattataaaa atattatttt
    23341 ctctaactaa gtaattccat actatacagg cttttaatac aaattgtcaa acacactgaa
    23401 atatacctat ttattccata tatatctttt ttacatatct ttacagtaaa aaaagtctta
    23461 atcttatgtt agtatagatt cacgggtaac cccctacact aaaggttaca aatcagtaac
    23521 atttttagaa tttttagatt aagaggtgtt taatgatgag aagaaatata ccaacaaaat
    23581 taaaaactat attagcttta ggagtgttaa ctttagcaac agcttcaagt gctgttactg
    23641 caagtgcttc atcacttgaa aataatgtaa atataagtaa caaaagttta gtatatagta
    23701 atactaaata caagttttct gcaaaaccaa attgttctgt aaaaccagaa cttaagcctg
    23761 ataaagataa ctcttgtaag gataagaatc ataatgacaa gaattgcaac aacactaata
    23821 aacctgagga caacaataat tcagactcta ctaataagcc tggtaacaat aaacctgagt
    23881 ctaataaacc tgaagacaac aataattcag gctctactaa taaacctgat aataataaac
    23941 ctgagtctaa taaacctgaa gacaacaata attcaggctc tactaataaa cctgataata
    24001 ataaacctga gtctaataaa cctgaagaca acaataattc aggctctact gataaacctg
    24061 ataacaataa acctgagtct aataaacctg aagacaacaa taattcagac tctactaaca
    24121 aacctgatga taataacaac tcaggctcta caagtgaaag tttttcagct taccaaaaag
    24181 aagttgttga cttagtaaat atagaaagag ctaaagctgg attaaatcct cttactcttg
    24241 attcttctat atcaaatgtt gctacaaaaa aatctcaaga tatgattgat aacaactatt
    24301 tttcacacaa ttctccaact tatggttctc catttgacat gttgaaaaaa tttggaataa
    24361 gctataagac agcaggtgaa aatatagcta tgggtcaaaa aactcctaaa gaagttgtaa
    24421 atgcatggat gaattctgaa ggtcacagaa aaaatataat gaatccaaat ttcagtaaaa
    24481 taggtgttgg agtagcacaa aaaagtggtg gttcaattta ttggacacaa atctttgtag
    24541 gataaaatac tctttttcca tacatatcaa aagagtatct ctaagtgata aaaaacacaa
    24601 atgagatact cttttgtatc tattttaaaa ttatcaaatc caaatataca tactataaat
    24661 attatttgtt ctcttaattt tagtgtgtag agcaatatat aaaaagtatt ggcaagatga
    24721 aaatggaaaa cttaaaaaga tatttaatag gtaaaaacag gatcttaata tcttcataaa
    24781 aaaaggctgt ctcaaaatag agataaggcc tttttcattg ttgaaatgat taaaatatat
    24841 ttgatttcat acaaaaagag tgcctcatga actaaaaagc tcactttgag acactctctt
    24901 ttatcaattc tatataagca ttataatact ttaatattat tcagctaatt ttttgtatcc
    24961 ttcatatctt tctttagcat cagcttcagc tttagcaaat aatccatcag cttcctctgg
    25021 gaattgttta gctatagcag aatatcttac ttgtgcaagt atgaaatctt tgaagcttgc
    25081 agttggttct tttgaatcta aagtgaatgg gtttttacct tcagctttta cttctggatt
    25141 gaatctgtat aaatgccagt atccgcaagc aacagcttgt gcttcgttag ctacagttct
    25201 tcccatacct tcttttaatc catggtttat acatggagca taagctatta ttaaagatgg
    25261 cccatcatgt ttttcagctt ctataacggc tttgataaat tggtttttat cagcacccat
    25321 agcaacttga gctacatata cgtttccata tgtcatagcc atcattccta aatctttctt
    25381 tctagatttt ttacctgaag cagcaaactt agccatagca gcagctggag tagctttaga
    25441 agcttgacct cctgtatttg aatatacttc tgtatcaaat acaagtacat ttacatcttc
    25501 accagatgca agaacatggt ctaatccacc gtaaccgata tcatatgccc aaccgtctcc
    25561 acctagtatc cattgagatc tcttaactag ataatctttt ctatctttta tagcttttac
    25621 taattcttta gctttttcat ctgtacaatc acaagctggt ttttctaata attcaacaac
    25681 cttagcactt gctactttag aagcttctcc atcatttcta gaatctaacc actctgtaaa
    25741 tactgcttta gcatcttcac agatatccat agaaactaac tctgtcatag catctactat
    25801 tttatttctt atttgcttaa cagctgtgta cataccaaat ccatattcag cattatcctc
    25861 gaataaagag tttgcccaag atggaccttt accttcatgg ttacaagtat aaggaattga
    25921 tggtgcagaa gctccccata tagaagaaca tccagtagca ttagctatca tcattctatc
    25981 tccatataat tgtgtaacta ctttaatgta tgcagtttct ccacatccag cacatgctcc
    26041 tgaaaactcc attaatggtt gtctaaattg gcttccttta acagtgtttg caggcataac
    26101 atcacctttt ggagctacct catcaggatt tactgcaaat gcccaattat ctaactctgt
    26161 atcaagttga gtatctattg gcttcataac taaagctttt tctttagctg gacatatgtc
    26221 agcacagttt ccacatcctg tacagtcaag tggactaagt tgcattctat attttaatcc
    26281 ttctaatcct ttacctaatg cttttttagc ttcaaatcct tctggagcat tagctaattc
    26341 ttcttcagta actaatactg gtcttataca tgaatgagga catataaaag aacattggtt
    26401 acattgaata cagttatcta ttatccattc aggaacatca acagctatac cacgtttttc
    26461 ataagctgca gtaccacaag ggaatgttcc atcttctata ccattgaatg tacttactgg
    26521 taagttgttt ccttcttgtg cagtcattgg tcttagtata ttttttataa attctggttc
    26581 tttaacttct cctttatctg catcaacagc attttcccaa gaagctggaa cttcaacttt
    26641 aactaaagca tttattcctg catcaacagc agcatagttc atgttaacta ttttttcacc
    26701 ttttttacca taagctttaa ctatagaatc ttttaagtat ttaacagcat cttcttctgg
    26761 tattatttca gctaatttaa agaatgcaga ttgcattatc atgtttattc tgtttccaag
    26821 acctatttct tgtcctagtt taacagcgtt aactgtatag aattttatat catttttagc
    26881 tatatattgt ttcatatgag ctggtaaatg ttcttcaagt cctgcttggt cccatatagt
    26941 gttaagtaca aatgtaccac cttttttaag accttttaat aaatcatatt gataaacata
    27001 tgattgttta tgacaggcaa tataatcagc ttcatctatt aaataagtcg ctcttattgg
    27061 agtatcacca aatcttaagt gagacatagt tataccacca gattttttag agtcataatc
    27121 aaagtaagct tgagcatatt tttcagtatg gtcaccgatt attttgatag cttgtttatt
    27181 agctccaaca gtaccgtcag aacctaatcc ccagaactta catcttatag ttcctttaga
    27241 agctattttt attggctcag atggagttaa agaagtatta gttacatcat caactatacc
    27301 tactgtaaat ccatttttag gttgttctga aactaagtta tcaaaaacag tttttatatc
    27361 tgaaggagta gtatcttttg aacctaatcc gtatcttcca cctattatca taggagcatt
    27421 ttcttttcca tagaatacat cacgtacatc taagtataat ggttcaccag ttgaacctgg
    27481 ctctttagtt ctatcaagta cacatattct ttcaacagta gatggcatag catctaagaa
    27541 atgtttcatt gagaatggtc tatataaatg aactttaact aaaccatatt tttctccctt
    27601 agcatttaag taatctatag tttcttctat tgtttctgtt actgaaccca tagctattaa
    27661 tacgtattta gcatcttctg ctccatagta atcaaataaa ctatgttttc tacctgttaa
    27721 attactcatt ttttccatgt atttttcaac tatacctact atattttgat agtatttatt
    27781 agaagcttct ctagtttgga aatatatatc tgggttttga gcagtaccac gagcaactgg
    27841 atgatttgga gataaagcat tatctctaaa tgcttgaact gcttcaaagt ttaataaact
    27901 tgcataatct tcattttcta gtaattcaac tttttggatt tcatgtgaag ttctgaatcc
    27961 atcaaagaaa tgtataaatg gtaatctacc ttctattgca gctaaatgtg caacaggtgc
    28021 tatatcagct acttcttgaa ctgaaccaga agctaataat acacatccag tttgtctagc
    28081 agccataacg tcttggtgat ccccaaatat tgataacgct tgagatgcta aagcacgagc
    28141 acttacatgg aatactcctg gtaataactc tcctgcaact ttatacatgt taggtatcat
    28201 taataataaa ccttgagatg ctgtaaaagt agaagttaaa gctcctcctt gtaaagaacc
    28261 gtggaatgtt cctgatgcac ctgcttctga ctgcatttca acaacattaa ctgtttgtcc
    28321 aaatatattt tttcttcctt gtgatgccca ctcatcaact acttcagcca tagttgaaga
    28381 tggtgtgatt ggatatatag ctgctacatc tgtaaacgca taggcaacat gagctgcagc
    28441 tgtatttcca tcaagtgttt tcataaactt agccataata tgtatcctcc ttgatagcta
    28501 caattgtact tgtttataac aagtctattt ttttatattt acatataatt tacacaacaa
    28561 aaatataaac acacatatag tcgcgcaaat actctatctt ctaatataat actacagatt
    28621 agataaaatt cctttgaaaa ttgtaaatta attacatatt taagtagatt ttttttaaaa
    28681 ttaaatatgt taagtttttt tcataatt
//
LOCUS       DAFKMX010000027        28383 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFKMX010000027 DAFKMX010000000
VERSION     DAFKMX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.027"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            260..964
                     /locus_tag="LZB08_001012"
     CDS             260..964
                     /locus_tag="LZB08_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314526.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            966..2453
                     /locus_tag="LZB08_001013"
     CDS             966..2453
                     /locus_tag="LZB08_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314527.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2658..4271
                     /locus_tag="LZB08_001014"
     CDS             2658..4271
                     /locus_tag="LZB08_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7314528.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4634..5464)
                     /locus_tag="LZB08_001015"
     CDS             complement(4634..5464)
                     /locus_tag="LZB08_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7314529.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIRPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5537..7264
                     /locus_tag="LZB08_001016"
     CDS             5537..7264
                     /locus_tag="LZB08_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314530.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7301..9163
                     /locus_tag="LZB08_001017"
     CDS             7301..9163
                     /locus_tag="LZB08_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314531.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9496..10185
                     /locus_tag="LZB08_001018"
     CDS             9496..10185
                     /locus_tag="LZB08_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBM7314532.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10290..10976
                     /locus_tag="LZB08_001019"
     CDS             10290..10976
                     /locus_tag="LZB08_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBM7314533.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            10993..12900
                     /locus_tag="LZB08_001020"
     CDS             10993..12900
                     /locus_tag="LZB08_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBM7314534.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKITVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13352..14041
                     /locus_tag="LZB08_001021"
     CDS             13352..14041
                     /locus_tag="LZB08_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314535.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14477..15016
                     /locus_tag="LZB08_001022"
     CDS             14477..15016
                     /locus_tag="LZB08_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314536.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15035..16078
                     /locus_tag="LZB08_001023"
     CDS             15035..16078
                     /locus_tag="LZB08_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314537.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16080..16916
                     /locus_tag="LZB08_001024"
     CDS             16080..16916
                     /locus_tag="LZB08_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314538.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17021..17695
                     /locus_tag="LZB08_001025"
     CDS             17021..17695
                     /locus_tag="LZB08_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314539.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17695..18747
                     /locus_tag="LZB08_001026"
     CDS             17695..18747
                     /locus_tag="LZB08_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7314540.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPIDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            18977..19825
                     /locus_tag="LZB08_001027"
     CDS             18977..19825
                     /locus_tag="LZB08_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7314541.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19815..21479
                     /locus_tag="LZB08_001028"
     CDS             19815..21479
                     /locus_tag="LZB08_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7314542.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGDGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLSIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            21528..22784
                     /locus_tag="LZB08_001029"
     CDS             21528..22784
                     /locus_tag="LZB08_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7314543.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            22807..24888
                     /locus_tag="LZB08_001030"
     CDS             22807..24888
                     /locus_tag="LZB08_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBM7314544.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSINEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            24925..26010
                     /locus_tag="LZB08_001031"
     CDS             24925..26010
                     /locus_tag="LZB08_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBM7314545.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKVVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            26064..27188
                     /gene="wecB"
                     /locus_tag="LZB08_001032"
     CDS             26064..27188
                     /gene="wecB"
                     /locus_tag="LZB08_001032"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBM7314546.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            27266..28240
                     /locus_tag="LZB08_001033"
     CDS             27266..28240
                     /locus_tag="LZB08_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBM7314547.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        11304 a         2797 c         4868 g         9414 t
ORIGIN      
        1 tctaaagtaa ttaaaaggca attaaggtga agtagttcaa gtatttctaa aataagttta
       61 ataaattcta taataattta ttttttgcct taaaattgcc ttaaaatcca aacaatttta
      121 aaactaatgt actataaaaa gctggtataa tttaaatata gtaaaaaata agatttatct
      181 tatctattgg gatttaatta tttaatcaat atatttatac catttaattt aaataaaaag
      241 tctattaagg agtgagctta tgagtaagtt aatttatata gcagatgatg aagataatat
      301 aagaaatttg gtaaagacat tcttaaaaaa tgaaggtcat gatgttatgg attttaaaac
      361 gggtgatgaa cttttagaac aatttaatat aaaggagtgt gacttagtaa tactagatat
      421 aatgatgcct ggctcaagtg gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt
      481 cccaataatc atgcttactg caagagatac agatatagat tatattacag ggataacctt
      541 aggtagtgat gattatttta caaaaccatt tagcccaatg tctttggtta tgagagtaaa
      601 atctatattt agaaggatag agtttgaaaa gaaacaaaat tatgataaat atagtaactc
      661 gatagatatg gaattaaaat ttggtgatgt aataataaat aaaaagaata aaatcgttac
      721 aagtaaaaat gtaaatatag accttacacc aaatgaatat aacctattga cttatttgtt
      781 tgaaaatata gatagagcag tttccagaga tgaattatta aacaaaatat ggggatacga
      841 tatagaagtt gagacaaggg ctgctgatga tacagtgaag agattacgta aaaaaatttt
      901 aaatacaaat attttaatag aaactgtatg ggggtttgga tttaggttga aggagaaaag
      961 ttaagatgaa gaatggaaat gacaagaata gaattggaaa gattaaatcc aaatttaaat
     1021 taaagttctc aaagaaaaat ataagcataa aaactaggat tttgttgagt atactttgtt
     1081 tgataattat agtatttatg ataatattag tatcttttaa tgtatttctt gatacatata
     1141 taaaaacaac tgcgaatgaa gaattgacaa agtctacaga aatagtagaa catatggata
     1201 gtaattttag acctattaag ccaccacaag atgatggaaa gcttcctccc aaagtattat
     1261 ctaattttat gagaaatgta caagataaag ttaggatggc agaaacacaa tcagatgctg
     1321 atgctatggt agttgattct aagtataacc tgatatttcc aacaagagag gatgatttct
     1381 taaaaaatgt tgatgagatg gagctgatta gggagtgtat tcaaaatgaa aaattagatt
     1441 taaattcaga tgcaaataca cggatatcta ccagaaataa ggattattat atttcaactg
     1501 taaaaattac tagtatatat ggtgaacaag acaagtatct tatacttttt atagacatat
     1561 caaaaactct aaatctagca caaaagataa atattgtatt aatatctgta atgtgttttg
     1621 ctggaatatt agcaatattt acagctgtga ttttatctga gaaaattgct aaaccgataa
     1681 aagagttgtg tgagtttgca aaaaaaatgg gtcaaggaga tttcaaaaga acctactttg
     1741 acttttcaga taaagaatta gtagagctct caactgttat gaataagagt gctgagtatc
     1801 tggataagta tgataatgaa caaaaaatat tttttcaaaa tgcatctcat gaattaagga
     1861 caccacttat gtctattaaa ggttatgcag aagctataaa atataatgtt atagatagta
     1921 aacatgcaag tgatataatt cttgaagaaa gtgataggtt aagtgatatg gttgaagatt
     1981 tattatatat ttctaaaata gataatataa ctaaagattt tgaacttgta gaatgtgatt
     2041 taagagaagt actttcaaat tgtggagcta ggcaaaatgt aagagctata aataagggga
     2101 taaaatttat ttatgaattt gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca
     2161 tttccaaagc atttatgaat cttatagaaa atgctttgag atacgctaag agtgaaataa
     2221 aaatagtgtg taagtataat caaaaaaata tagttgtgat aatagaagat gatggaattg
     2281 gaatcaagaa agaagattta cctcatgttt ttgaaagatt ctataaggga gtaggaggaa
     2341 atcatggaat tggattatca atagttaaat ctattgtaaa taagcatggt gggcgtatat
     2401 acgtagaaaa tggcaaaaaa ggagctaaat ttactatatt atttaaattg taaaaaaatt
     2461 agagaaaata tagagatgtt tgaagttgtt ttttatatat atctgttgta tactgttgaa
     2521 aaaagaacaa tattaagctg acatatatta cgatagttga catttaaagt aaatgcttaa
     2581 atatagatac gaaaggaaaa gtaaaatctg aagtgtattt aggtttaagt aagcttctta
     2641 ttttataaaa aatatgtatg aaagaacagc tgatttttaa tattacaaat ttagtaacgg
     2701 ttgtatttga agcttttgtc atccatatgt ttttatcaga ctttttaggc cagaaagaag
     2761 ggtatataaa agttgtaaga tatgctaaac tgggttttat aatatgttta ggattctgta
     2821 atttaataac tttaaaccct aaaatcacaa tgcctttaat tttcatcctt attttttcaa
     2881 catcattttt atataaagga aatttaaaga cacgattgtt tactactgtt ttattaagta
     2941 tattctttat actgtcagaa attgtagtta caagtatttt tgttttattt gtaaaagaag
     3001 gctttgaaat aatgcttgaa aataatagta taagagtatt ggcgacaatt ttatctaaaa
     3061 ttgtcttttt gttgacttgt aaaattattt gtctatttaa aaaagatgta catttagata
     3121 tgcccataaa gtattggctc cctttatttt taatacctat atttagttta tttctgtctg
     3181 tttctatctt tgatgtaagt aaatttttta gtcttgaaag tttaaaattt ctaagcttaa
     3241 tatctagtgt tggtatttta tatattaatt ttattgtctt ttatttattt aaatttatta
     3301 tagataaaac taaactatct atgaaatatg aactacttga aaaagaaata attcataagg
     3361 aagaattaag acttagtaat gagtgttata aaattattgt tgagcagact gattctgtcg
     3421 ttttcgaatg gaatatcaaa gaaaataaat catttgtatc gcaggcttgg actgaaaaat
     3481 ttggatataa caatgcatgt aagaatattt ttaaagaaat taaagataaa gacttagttc
     3541 attctgaaga taaagctata tttgaaggtt ttcttgaaag tattaaaaaa aagaatatgc
     3601 acaatcaagc agtatatcgt ttaaaaaaat ctaatggtga atatatttgg tgtagaacat
     3661 caattacaag tatttataat gatgaaaatg aacttttaag agttgtagga gtaattgtag
     3721 atgtagatag tgatataaaa aaatatgaag agttaagaac tagagcagaa agtgactcat
     3781 tgaccaatat atacaataaa ggtacatttg aaaaattagt ggaagaaact atagttatga
     3841 atacaggtga taaaaaagat gcattattca ttattgattt ggatgatttt aaagaaataa
     3901 atgataattt tggtcatcca tttggtgatt ttgtactaaa aacatttgct gataagatac
     3961 aaactagttt tggtagtaaa gacttagtcg gaagaattgg tggagatgaa tttgttgtat
     4021 atatgcaaga ttatgttaca gaagtaaatt tgcataaaaa agctaaagaa ttaaatagag
     4081 tactttcgga taattacact gatttgagtt ttagttttga tgcttctgtc agtataggta
     4141 ttgcaaggta tcctcaagat ggaacttcat tttttgaatt gtttaaaaat gcagatagag
     4201 cactttatag cataaaagca tcaggtaaaa attcatattg tttatttgaa gaagaactat
     4261 atgtacagtg aatttgaatt tagattttat gtgagagtaa gatttatata tacttgtggc
     4321 tgtctgattt ataaaatatg ttactttgta tatttttata tgtttcagta aggttaatta
     4381 acataagcaa aggtgtaagt aaatttatat tagtttaagt ttacttacac ctttataaaa
     4441 atacaaataa tagattcatt tacattctta aatgattaaa gctctttacc ttctaaacat
     4501 gcataacata tcgttataat tccagtaaat aagactaaaa tacccattcc taagattaca
     4561 gtttcattat ctattactcc aaatgttttt aaagtaactt atttcgcttt gtatcataat
     4621 aatttgtctc tttttacatt ttaacttctg ctgagatttg agttagatat tcattagaat
     4681 ctcgcacact tatatcatat agaagatatt cttcataaac aaaatcccca atacgtaaat
     4741 tattctgatt aaaaaaattt aataactttt cataagtctt gtaaatagtt tcatagctcc
     4801 ctttatgata agcagttaca taaagtccct ttggtctgat tgatacagga tatttcttgt
     4861 actctttatt aacttttgta aataaatatg aatatgattc aaagtcttta ttaattatat
     4921 cttcaccttt aatgattgag ccaatcgtac cataatcact caattcatag ttgtccataa
     4981 agttaataca ttcatataaa aaattttctt cctcatctcc taaaaattgt tctttatatg
     5041 taacaggtgt tttgaccaag tactcttctt catgttcttt taaagtaata gtattcgcat
     5101 ctatattaca ggcattttta gtgaatgaaa tagtagattc cattagagct tgaatattat
     5161 ttagtttttc aatttcattt ttaatatcaa tggttttctt ttctaaaagc tcaattagta
     5221 aattaggtgt tcttttgtct atataagctt taatttcttt taaaggcatt tttacttccc
     5281 taagtgaaga aattatacca aaaatgctaa gttgatggta tgagtagtat ctatatccat
     5341 tttctttctt taattctggt gagaaaagac ctatatcatc ataatggaaa agtgtttttt
     5401 tattgattcc acaaatcttt gcaaattctc cagttgtaaa ataatttttt gaaacttgac
     5461 tcataattat aattcctcct tgactatcct gttacagtat agtttatcat agtattttgt
     5521 aaaggagtgt gattatatga aaaaattaat tcatttttta aaaccatata gagttttaat
     5581 tgttgtagtt ttgatattta cgtttttaca aacacttgga actttgtata taccaacact
     5641 tacagcaaat atagtaaaca atggtgtagt aaagggagat atagattata ttgtaaaaac
     5701 aggtttaatg atgatgatag tagctggtat aacagcttta tcagctgtgt tagtttgtaa
     5761 agtatcagca aatctttcat ctggattttg tagagatatt agagaagcag tatttataaa
     5821 atcacaagat ttatcaataa atgattttaa taatataggt acagcatcaa tgattactag
     5881 gagtactagt gatattactt taataggtca gtctgtgttt atgtttattc aattagtatt
     5941 accagcacca attataactg tttctggtct attcttagca tattctatag ataaagcaat
     6001 gacaattatt attgttgtag taatgttttt attcatgctt tcagcatttt tagttggtaa
     6061 aaagcttata aagttgttta aaatgatgca aataaagatg gacaatatga atagggtgtt
     6121 acgtgaggta gtaactggag taagagttat aagagctttt aataggagtc gttttgaaaa
     6181 aaagagattt gatagaactg caatagatta ttctgaaaca gcaatttcaa taaataaaat
     6241 atttgcagta cttatgccta ttgtaatgtt aataatgaat ttaggtatag taagtattat
     6301 ttggtttggt gggatgaggg tcagtaatgg aaatatggaa ataggtcata taatggcact
     6361 tgttgaatat tgtatcttaa ttttattcta tttaatcatg ggagtaatgg tatttatgta
     6421 catacctaga gcaggtgcat gtgcagatag agtaaatcag atattagata tagaacctga
     6481 aattgtagat ggaaatggtc ataaagatac tgtctcagag agaggacatt tagtatttaa
     6541 aaatgtaacc ttttcatatg cacaatcaga agaacctgtg ttaaataata taacatttga
     6601 agcaaaatct ggagaagtaa ctgcaattat aggaagcact ggctcaggca aatctactat
     6661 tgcaaatata ataccacgtt tttttgaaat tcaatctgga gaaatatcta taaatggtca
     6721 ggatattaag aagattcctc aaaaagaact tagagataag attggatttg taccacaaaa
     6781 agctttttta tttagtggaa caatagaaga aaatataagg tatggaaaag aagatgcaag
     6841 tattgaagaa gtaaagcatg ctgcaagtat agctcaagct gatgaattta tttctgatat
     6901 ggaagacaag tatgactcat ttgtagcaca aggtggaaat aatttgtcag gtggtcaaaa
     6961 acagagaata tccattgcaa gagcgttagt tagaaagcct gaagtatatg tctttgatga
     7021 tagtttttct gcattagatt ttaaaacaga taaaagactt agaaaagcgt tgaaaaatga
     7081 aatcaaagat tcatcagcta taattatagc acaacgtata agtacaatca tggatgctaa
     7141 tcagattata gttttaaatg atggaaaaat tgtaggtatt ggaaaacata aagatttact
     7201 tgaaaattgt gaagtttata agcaaattgc ggattcacaa cttagtaaag aagaacttgc
     7261 ataaaatgaa gaattaatct gatatttgga ggtgtatgat atgagtgaaa aaagaataga
     7321 tgaacaagat atagaagtac ttagttttga agatggcatg tcagcagaaa aagctaagga
     7381 tgcaaaaaaa actactaaga gattattaaa atatatggca aaacaaaagt ttaaaatatt
     7441 aattattttt atttcagtat tgataagttc agcgcttaca gttttagcac ctatggttat
     7501 gggaaaagct attgaccaac tatttaatgg aataaaaact gcagtccaaa caggtacaaa
     7561 attcagtgtg aattttagta ctatgggagg gatagtatca atacttctag ggttgtactt
     7621 aattagtgca gtatttatat atattcaaca gtatattatg tcaggagttg cacaaaatct
     7681 agttcttagc atgagaaaag atttaagtga taaactcaat aaacttcctc ttaagtacta
     7741 tgattctcat aaaaaggggg aaacacttag tatagtaaca aatgatttag aaaaagtagc
     7801 agatagttta caagaaggat taatgcaact tattacagca gtagttacag tagttggttc
     7861 tattgtaatg atgataagta taagtgtacc acttacaata gtatcagcta taacattact
     7921 tgtttcattg ggtattactg ttgtaattgc aagaaaatca caagaaagat tctctgaaaa
     7981 tcaaaaggca ctaggagagt taaatagcaa tatagaagag atatttactg gtcaaatagt
     8041 ggttaaagca tttagtaaag aaaaggatac tataagaaac tttaaaatat taaatcaaaa
     8101 tctttataat gcaagtagaa aagcacagtt ttcgtcatat gcaatatctc caattattag
     8161 atttataaac ctaattggct atgttattat agcagttgta ggtgggatat ttgcatcaac
     8221 tggagcaatg actcttggta gtatacaagc gtttattcaa tatgtaaatc aagcatcaga
     8281 gcctactaca gaaatatctt atattgtaaa tatgcttcaa gcagcaatag catctgcaga
     8341 gagagttttt acagttatgg acgaggtgga agagataaag gatgaagaat cttcaaaggt
     8401 tattagtatg cctaaaggta aagtacaatt tgagcatgtt aaatttggat atagtgatga
     8461 ttttattctt atgaaagata ttaatatcaa tctaaatgca ggagataaga ttgcaattgt
     8521 tggacctaca ggagctggaa agacaactct tgtaaattta cttatgagat tttatgagat
     8581 acaaggtgga agaattacta ttgatggtgt taatataaaa gatttaaagc gtggagaact
     8641 tagaactatg tttggtatgg ttcttcaaga tacatggcta tttaatggaa gtataaaaga
     8701 aaatattgca tatagtaagt cagatgccac aatggatgaa attgtaagag cagcaaaatc
     8761 agcaagagta gaccatttta taaggacttt accacaaggg tatgacacta tattgactga
     8821 ggatgcttct aatctatctc aaggtcaaaa gcaacttcta acaattgcca gggcaatact
     8881 ttcagaccca tcagtattga ttcttgatga agcaacatct agtgtagata ctagaacaga
     8941 ggtagaaatt caaaaggcaa tgaataactt gatgaaggga agaactagtt ttgttattgc
     9001 acatagatta tcaactattc gagatgctga tttaatatta gtaatgaaag aaggaactat
     9061 tattgaacaa ggaagccata aggaacttat tgaaaagaaa ggattctatg aagaacttta
     9121 taacagtcaa tttacaagtg aatatgatga agatgttgta tgattatcaa gttttaggca
     9181 actaaatgat tgataaatat taagttaaat atagtaacat gtaaggttat atggaataag
     9241 agtatttctc tatttcatat aacctttttt atttatcaaa ataagataaa atttcctttc
     9301 taaattagat tgaagaatgt atttatcaat ataaaagtaa aataatcatt attattagtg
     9361 aagtaaatat tgtttgccac atatatgcca taaagatgca cttattttac ggagatttaa
     9421 caaagtagtg taaagtatac tctaaatata aaataaatca aatagagtta aataaattga
     9481 gaggtgatat aaatagtgaa taaaaaattg aactatagat ttgaaatgaa gcacaaaata
     9541 actgaagcag atgtgttagc tttaaaatca agactctatc caataatgaa gaaagatgaa
     9601 aatgcatcta aagatggaaa atatcttatt aggagtttat attttgatac tcctgaagat
     9661 aaagcacttt tggataaatt aaatggcgtt gcaataagag aaaagtttag gataagattt
     9721 tataataatg actgctctta tataaggtta gagaaaaaaa tcaaacatta caacatgaca
     9781 tcaaagctat caactaattt aaccaaaaaa gaagtaataa atatactaaa taatgatatt
     9841 gaatttctaa aagagtcgac aaattgttta ttaagagaat tttatttgaa attaaaatgt
     9901 gaaagattag aggcaaaaag catagttgat tacaatagag aagcttttat ttatccagtt
     9961 ggaaatgtaa gagttacaat cgatagtgat attaaaactt ctgtaaattc ggtagattta
    10021 tttaataaag atttgccaac tgtttcggta attgatgaga atatgactgt attggaggtt
    10081 aaatatgatg aatttatacc agattttata aaggatttaa ttcaaattaa taagactacc
    10141 tctacagcag tttcaaagta tgcttcaagt agattatata tttaagaata aaggctatga
    10201 tttatataaa gatatacata aataaggtta aaaaacaaat aagttaaaaa taaataaaat
    10261 aaaaaataaa taagttaaaa ataggaagga tgattttaat tatgacattt aatgatattt
    10321 ttaaatcaag ttttatagaa aacgtatcag gtttttcatt tgtggacagt gctttagcac
    10381 taggttcagc ttttttagta ggtttgttta tatatatggt ctataaaaag acttatatgg
    10441 gaattatgta ttctcgacca ttcaatgtgt cgcttgtagc acttacaatg cttacaactt
    10501 ttgtaatatt ggcagttact tcaaatgtag ttttatcctt aggtatggtt ggtgctttat
    10561 ctatagtaag atttagaaca gcaattaaag acccaatgga tttagtattt ctattttggt
    10621 cattgggttc aggtatagtt ttaggagcag gtttgattcc cttagctgtc atgggttcaa
    10681 taattatggg attgattcta atattctttt caaataaaac aatatctgaa actccatata
    10741 tattgatggt aaattgtaat aatgaagatt cagaagatat agcaactgat aaaattaaaa
    10801 aagtatttac taaatatcaa ataaaatcaa aaagtgttac accagaaaaa ggaatagaat
    10861 tagtgtttga agtaagaatg aaagatggag aaacaagtct tataaatgac ttgagtcaag
    10921 tagatggagt tactaatgca gttcttgtaa gttataatgg tgactatgta gcatagagag
    10981 gagtaatctt ttatgaaaga taaaaaattt acccttctta tctcgattat gattatattt
    11041 ttatgtgctg tagttggagt ttatagtaca tctagcaaca aaagtgttga tttatatagt
    11101 gatgtatata ttgaaaaata ttttaacaga gacaaggtta tggaagttaa tatagagata
    11161 gatgaaagtg acttgaagga tatgaatgaa aatgctataa aagaagaatt taaggttgca
    11221 aaaataactg tagatggaga tacatatgga aacgtaggta taagaactaa aggaaattca
    11281 agccttatat ctgtagcaaa tagtgatagt gatagataca gctataagat taattttgat
    11341 aagtataata ctagtcaaag tatggaaggg cttactcaat taaatcttaa taactgttac
    11401 tctgacccat cttatatgag agagttttta acatatagta tttgcgagga aatgggatta
    11461 gcgactccag aatttgcata tgctaaagtc tctataaatg gcgaatatca tggtttgtat
    11521 ttggcagtag aaggattaaa agagtcttat cttgaaaata attttggtaa tgtaactgga
    11581 gacttatata agtcagatga aggaagctcg ttgcaatata aaggagatga cccagaaagt
    11641 tactcaaact taatcgttga aagtgataaa aagacagctg attggtctaa aattacaaaa
    11701 ctattaaaat ctttggatac aggtgaagat attgaaaaat atcttgatgt agattctgtc
    11761 cttaaaaata tagcaataaa tacagcttta ttaaaccttg atagctatca agggagtttt
    11821 gcccataact attatttata tgagcaagat ggagtatttt ctatgttacc atgggatttt
    11881 aatatgtcat ttggtggatt tagtggtttt ggtggaggta gtcaatctat agcaattgat
    11941 gaacctacga caggtaattt agaagacaga cctctcatat cctcgttatt aaaaaatgag
    12001 acatacaaaa caaaatacca taaatatctg gaagagatag taacaaaata cctagattca
    12061 gactatttag agaatatgac aacaaaattg catgacatga tagcatcata tgtaaaagaa
    12121 gacccaacag cattttatac ttatgaagaa tttgaaaaaa atataacatc ttcaattgaa
    12181 gattctagtg ataataaggg atttggtaat aaagggtttg acaacaataa ctctaataac
    12241 agtgattcta ataataattc taatagtgaa aataagcgct ctggaaatca aagtgatgaa
    12301 aaagaagtta atgctgaatt aacatcaagc gtagtcaaag ctaatacaga taatgaaact
    12361 aaaaataaaa ctacaaatga tagtgaaagt aagaataata cagataaaga taaaagtgga
    12421 aatgataata atcaaaagct agaaggtcct atgggtaaag gaggtaagtc aataccaggg
    12481 gttttggaag ttgcagaaga tatgagtaaa actataaaat ctcaattaag tggagaaact
    12541 tcttcgacaa agcaaaactc tggtgatgaa agttcaagtg gaattaaagg tagtgaaaag
    12601 tttgatgagg atatgagtgg tatgccagaa ccaccggagg gaatggatgg taaaatgcca
    12661 ccaggaatgg gtaatatgga taagggagat atgaatggta aaaatggcaa tatgaatatg
    12721 gatagaaatc aagataatcc aagagaagct ggaggttttg gcaatagagg aggaggctct
    12781 gtgagtaaaa caacaacata cttcaaatta attttaggtg gagcttcaat gataataatg
    12841 tcgattatgt tagttggtgt atcaagggta aagagaagaa gatttataaa gtcaaaataa
    12901 gattaaaatt gaatataaat aattttaata ctgttaagtc taaatattta cttagcagta
    12961 ttttttagaa aataatgtgt ttgattaata atataaggta ataaattgat aaaaattaaa
    13021 tacagcatat taataataca taataaagta ataaaaaaac tgtttaagta taataactta
    13081 tatttagaca gtttttttca tttataaata atataaatga aattgaaaac atttgaagga
    13141 tagaaattgg ttggataaat gtataaatta cttcaccaaa aaataatgac aaaaattgta
    13201 actaagttgt aataaaatgg tagttattta gcataaaata atgtatcata gaagtatatt
    13261 ataaaattaa taaaataaga tttttgtaag aaaactgctg gcttttgata tttagagtga
    13321 gtagttaaat tatgtttagg aggggtagaa tttgttgcaa aaagtattat ggaaattttt
    13381 aaagctagta gtcggtttat ttatatgttc agttggtata gtattgacga taaattgtaa
    13441 ccttggatta tctccctggg atgtatttca tcaaggactt tcaaatcata taggaataac
    13501 tataggaact gcttctataa ttgtaggctc tatagtagtt attgctgatg tcgttcttgg
    13561 agaaaatgta ggatggggaa ctgtttttaa catgctacta attggatttt ttatggattt
    13621 acttttatat agtaatttaa ttccagaggc tgatagttta tttgtaggaa taattatgtt
    13681 gatattagga ttggttcttt tgtctgttgg aatggtattt tatatgggaa gtggtttagg
    13741 aagtggacct agagatggtt tgatggttgc aattcaaaag aaaacaggaa aatcacttaa
    13801 attaataaga ggtactatag aagttggagc attaatagta ggatttttat taggtggaaa
    13861 agttggtata ggaactataa taagtgcatt tggattgggg tatttcacac aaatggtatt
    13921 tggcttattt aaattagatt gtagtaagat taaacataga tttatagtag atgatataaa
    13981 atttatcaaa gtgtatatga gtggtgagaa aaaagctgct gttcaaaata tagttaagta
    14041 atttaagaat aaattcaacg aaatgttaag tattttaaat taacattttg tttagataga
    14101 taaattgaat attaaaagaa tattaaaata tgaggattgc tagaacacta taaaatatag
    14161 atttctagca attttttatt tgttcaatat ttttttagta ctagaagtct tattctctat
    14221 ttaatgaatg aattaattta taattagaga tactaaactt tatagatatt aatataaaca
    14281 cctttaaact gtgtattttc caaagccttt aaaatgcttg tatttattct aaataaaaaa
    14341 ataaaaaaat gtcaaaaaaa ctcttgtatt atatccaaat tttatatata attacctatg
    14401 ttgtttagaa attttaaact caatgtttag taaacaaaat gtttagtaaa ataaaacaga
    14461 aactaggagg tgcaacatgg atataggtga aaagataaaa agattaagaa cagaaaaaca
    14521 actgacccag gaagagttag ctaatagatg tgaattgtca aagggattta tttctcaatt
    14581 agaaaataac ttaacatcac catccatagc tacccttata gatatacttg aaatactagg
    14641 tacaaattta agggaatttt ttaatgaaat agatgatgaa agaatcagtt ttacaaaaga
    14701 agatatgttt gaaacagaag atgaagattt aaaatataaa ttaaagtggt taattccaaa
    14761 ttcacaaaaa aatgaaatgg agccaataat aataacattg tatccaggag gacagtataa
    14821 agaagaaaag ccacatgaag gtgaagaatt tggatatgta ttagcaggtt caatatatgt
    14881 ccatataggc gaaaagaaaa ataaagtaaa aaaaggagag agtttttact ttagaccgaa
    14941 agcaaatcac tatatatcaa acgagggcaa aactacagca aaagtgattt gggtaagtac
    15001 gccaccatca ttttagaata gaggtgttta ataattggta gaaaatataa tagaattaag
    15061 ggatatatct aaacattatg aagaacttac tgttttagat aatttcaatt tagatataaa
    15121 gaaaaatgag tttctaacat tacttggacc aagtggatgt ggaaaaacta caacattaaa
    15181 aataatagca ggatttgagt atgctgatga tggtaaagtt ttatttgaag gtaaggagat
    15241 aaataatttg ccaccatatg aaagacaagt aaacacagta tttcaaaaat atgctttatt
    15301 tccacacatg gatgtttatg aaaatgtagc ttttggatta aaaattaaaa agactccaaa
    15361 agatgtaata gatgctaaag taaaagaggt tttgaaactt gttgcactag aaggttttga
    15421 aagacgacaa gttgaatcat tgagtggagg acaacaacaa agaattgcaa tagcaagagc
    15481 tctagttaat gaaccaaagg tattattact agatgaacca cttggagcac ttgatttaaa
    15541 gttaagacaa gagatgcaaa tagaattaaa gagaatccaa aaaaaattag gaataacatt
    15601 tgtttttgta actcatgacc aagaagaggc tcttactatg tcagatacta ttgttgtaat
    15661 gtataagggc aaaattcaac aaatgggaac accacaagat atatacaatg aacctaaaaa
    15721 ttcatttgta gctaagttta taggtgaaag taacatattt gatggaatta tgatagaaga
    15781 ttataaagtt aatttctgta atagagattt tgagtgtgtt gataaaggat tcgaaaaaaa
    15841 cgaaaatata gaagtagtaa taagaccaga agatataaaa atggtaaagc cagaagaagg
    15901 aatgttaaag gggaaagtta catctacagt gtttaaagga gtgcattacg agatagaatt
    15961 gaatgaaaat ggaagattgt ggatacttca taatactaaa aatgctgagg ttgggactga
    16021 actaggcatg gatatatatc cagaagatat ccatataatg agaaaagaga ataactaaga
    16081 tgaaaagaaa atctttttta gcatatccat atgtagtttg gagtgcgata tttgtaataa
    16141 ttcctttaat attagtagta ttttttagtt ttacaaaaga atcaggtgga ggttatgcct
    16201 ttacacttga gaattataaa gaagtaattg accctatata tatgaaagtt tttgggcgtt
    16261 ctattctttt agcaggagga gctacattaa tatgccttat agttggttat ccagtggcat
    16321 atataatttc aaaggctcgt gtaagtagaa gaggttcatt aatattattg tttattcttc
    16381 ctatgtggat gaactttctt ttaagaacat atgcatgggt tgctatatta ggaaaaaatg
    16441 gacttttaaa tacattttta ggttggtttg gaatacaacc attagcaatt ttgtatacta
    16501 attttgccat acttttaggt atggtgtata actttttacc atttatggtt cttccaatat
    16561 atacagcact atcaaagatg gataatgatt tgataaacgc tgctcatgac ttgggtgcaa
    16621 ataatatgac agtttttaga aaaattatat ttcctttgag tttgccaggt gtaatgtctg
    16681 gaataactat ggtatttatg ccagcagtca ctacttttgc aatatcaaga ctgttaggtg
    16741 gaggtaaaat aatgctggtt ggagatttga ttgaacaaca gtttacagtt gttggagatt
    16801 ggaactttgg ttcagctata tcgatattta tgatgatagt aatattaata tctatgtcta
    16861 taatgtcaaa atttggagat gaatctgaca aagaaggcgg tgggttacta ttttaaaatt
    16921 aaagaaaata acttctaata tatatttagc attagtattc ttatttttgt atgcacccat
    16981 atttgctttg gtactatttt ctttcaatga ttcaaaatca atggcaagat ggaatggatt
    17041 tacatggaag tggtatggtc aattacttca aaatgagagt ataatgagtg ctttatatta
    17101 tactattgta atagctatat tagcgtctgt aatatctaca atagtaggaa ctataagtgc
    17161 tataggaata cataagatga gaggtaagag taagaaatta atactaaatg taaactatct
    17221 tcctatttta aatacagaga tagttacagc agtagctctt atgagtctgt ttgtatttgt
    17281 aaaaatggaa tttggattta caacaatgct attggcacat ataatgtttt gtttgccata
    17341 cgttatactt tcagttttgc caaaaatgaa gcaattacct gataatatag aagatgcagc
    17401 tatggacttg ggagcaacac ctatatacgc tcttagaaaa gtaattttac cacaaataaa
    17461 accaggtata gtttctggat ttttaattgc atttactatg tcaatagatg actttataat
    17521 aagtttcttt aatgctggaa atggtgtaag caatctttct atagaaatct atggaatggc
    17581 tagaagaggt ataaaacctg aaataaatgc attatcaaca ataatgtttg cagtggttct
    17641 aggtttactt ttactagcta ataaaaaaga atctatagta agggggatta aataatgatt
    17701 aagtttaaaa aagcaatatc ccttatagct gtaggtatga tgacagttac tttatttaca
    17761 ggatgtaata aagcaactga ttctacaaaa gttttaaatg tatataatgt tggagattat
    17821 atagatgagt ctcttataga taagtttgaa aaagaaacag gaatagatgt tcaatattca
    17881 acttatgata ctaatgaaat gatgtatcaa aaagtaaaga gtggtagtac acactatgat
    17941 ttagtatttc cttcagacta tatggtagag aaaatgaaaa atgaaggtct tttggaaaaa
    18001 ttagatttta agaatatacc taatatgaaa tatttagata agagtttttt aaatcctgta
    18061 tacgacaaaa ctaatgaata tagtgttgca tatatgtggg gaactcttgg aatactgtac
    18121 aataagaaag aagttaaaga ccctatagat agctggaata tactttggaa tccaaagtat
    18181 aagggaaata taatgatgtt tgactctgtt agagatacta tagggataac tcttaaaaaa
    18241 ttaggatata gcatgaatag tgtaaatcca aaagaaataa atgaagctaa aaatctttta
    18301 atgaaacaaa aggatttagt attggcttat gtaaatgatg aaggtaagga tagattgctt
    18361 ggtggagaag tggcaatggg tatgctgtat tctggagatg cagttacact aatggagcaa
    18421 aatcctgacc ttgattatgc tatacctaaa gagggaacta ataaatgggt agatgctatg
    18481 tgtgtaccta caacagcaca aaataagaaa gaagctgagc tgtttataaa ctttttatta
    18541 gacccagaga atgcaaaagt aaatgctgag tacattgggt attcaacacc taatacagga
    18601 gcactaaaat tattagaccc agaaataact cagaatcctg ttgcatatcc atctaaagaa
    18661 gtacttgata aatgtgaaac ttttgaagat attggtgaaa atataaaatt atatgacaag
    18721 gcttggatag agttaaaatc aaaatagggt cttgttgttt taagtaaatt tacttaataa
    18781 atacttaata aaactgttct tttttagaaa atttattttc taattattga acagttttat
    18841 tttttgttta agttatattc atatgcaatt taaatagttt attaataaat gaaaacttat
    18901 ttgtataatg actttaataa taattagaaa aaatgggcaa cttaatagaa taattggagg
    18961 tgtaaattta taaaaaatga ataggataaa taaaaaaata gatttattaa tattggcttt
    19021 gctcgttttt atatttttaa ttgttggaat agtaatacta tcgattagga cagaaaataa
    19081 attgaatgtg ttcataatgt tagcaataat tttttttata ataatgttaa cttatttatc
    19141 aaattcagta gtggggttga taacaagttc aataattata tttatgtata catcctatat
    19201 tttatataac aatattactc acaatatgga tgttgaattt atatcatata tgtggataat
    19261 agcaacacca gtatcttcaa ttataatggg taatctgaac aaaagcataa atgaattgca
    19321 aaatacaaat aaaaaactat ctgaacagta taaggaatta gtgactattg atagtgaaac
    19381 tggacttaga aatttaaaga ttttttataa tgatgtaaat atggagataa gtaaatctat
    19441 tagacataat actgattttt ctcttatgat agtaaaatta ccatactacg gaaacctaca
    19501 gactatattt ggtgaaaaca aaactaataa aatagtaaaa tatattggtt caaatattat
    19561 agaatgtaca agaaatgaag atataattta tagtttacaa aaagatatga tagggatact
    19621 aatgccaaat acatctttgg aaggttcaaa agttgttaaa gacagaataa agaaaaggat
    19681 aaaagaatta aacttagatt taaacaatag gggaaaatat gtaaatatag atgtcaaaat
    19741 tgcattttta caatataaga gtagttttgg agatagtata aattttaaga atatagttga
    19801 agaggaactt caatatgatg tttaaaatat ttaataaaag tctattaaaa ttagtagttg
    19861 tattatcaat agtactgaca atatcaaatt ttagcttatc ttcatatgca tcagaaatta
    19921 cttccagcac agataaggta ttaatcatat atgattctaa gaaagaaaca gcttataata
    19981 gagatatttt aaatattatg aggactctat tgggaagatt tagttcagat atagagctat
    20041 taaaattgag taactatgat ggtgagataa ataaaaatta ttacagtcat atatttattt
    20101 taggaatcaa tgaaaactca tataataatg ataaaaacac taaaaacttg attagttcct
    20161 taaattcata taaaggaact atatgttggt taggagatgg tatagaaaat cttttggaac
    20221 ataaaaaata caatttagac tatgtaggaa aaacaaacaa tatagttagt gtaaattata
    20281 gaggtaaatc atataacttg gatgaacatt atgtttttaa cattgtagaa tcaaaagata
    20341 caagtaataa agtaatagga agtattaatg ataccttaaa taagtatcca tatataataa
    20401 atgataaaaa tcttttttat gtatcaaaat tagatttaga tggagtttta ttttatatat
    20461 tttgcgattc acttaatgat atttttaata ttaagacgtt tgataaagga cgtatatttg
    20521 taagaataga agatgtacat gcttttagag aaccaaaaaa tcttgtagag atagcggatt
    20581 atttaagtag taagaatata ccatttacga ttgcattaat accagcatat gtaaatccta
    20641 aaaatcataa agtaataact ttatcagaaa gccctgaaat tgtcaaagca ataaaatata
    20701 tgcaagataa aggtggaact gtgatattgc atggatatac tcatcaatac aagaaagaag
    20761 aagtttctgg agaagggtat gaattttggg atggtaaaaa agatgaaccc ttaaaagaaa
    20821 atatgaagat atttgtcaaa gatagagttt tgaatggtct tagagtatgt atagagaatg
    20881 gaatttatcc tcttgcattt gaagcgccac attatgcaat ggaatcagag ggatataaag
    20941 aattaaaaaa atatttttct acttatatgg ggcaacatca aaataatgat aaaaaattta
    21001 gcacaaatac atatccttat attattaggg atacagaaga gtttaatata tttatacccg
    21061 aaaatttagg atatatagac ccagaagata agtttacttt tcaaaatata aaagaaaatt
    21121 tagataaact atccattgta agaggtttta gtggagggtt cttctttcat tcatatttaa
    21181 gtatagagta tctaaaaaac acaatagaat atttagaaaa acagaatata gaatttatga
    21241 atttaagaga ttttaataat tgggtaaaag ttgatgaaat acaaataaga aataatggag
    21301 atgaaattat agtcaactat gataaggact tagatgaact aactaaaagt gatactagat
    21361 ttaaatccat atccaatatt agtaaaattt taatattcat agtatctata agtgttttaa
    21421 tatttgtgat tatatttata tattttaaga gaatagataa aaagaaattt ttaaaataaa
    21481 tgattttata ttagaatcaa tattttaaaa tattttagga gaaatttatg agagatttaa
    21541 atgtatcaga gtatttgttc gtatttagtc tgtttagtat atggtcttta ttgcttataa
    21601 atattatttt agctatgggt ggatatatat tttattttaa aaactttgat aaagaaataa
    21661 aagaaattga tgaatatcct atgatatcaa tacttgttcc tgctcataat gaagctaaag
    21721 tgataggtag aactgtggag tctttgctat tacttaatta tcctaagagt aagatggaac
    21781 ttattgttat aaatgacaat tcttctgata atagtaaaga aattttagaa aatataaagg
    21841 ataggtacaa caattataat ttcactataa taaatacaga tagtttaaca ggtggaaaag
    21901 gaaagtctaa tgcattaaat attggatata cgatttcaaa aggagatttt atagctgttt
    21961 atgatgcaga taacacacca gataaaaatg cacttagata tctagtacaa actatagtaa
    22021 tgaatgatga attaggtgct gttattggga aatttaggac tcgaaataaa aataaaaacc
    22081 tattaaccaa atttataaac atagaaacat taagctttca atggatgtca caagctggta
    22141 gatggcaact ttttaatcta tgtacaatac caggaactaa ctttatattg aggagaagta
    22201 tcatagaaga aattggagga tgggatagta aagcaattgc agaagataca gaaataagtt
    22261 ttaggatata taaattggga tacaagataa agcttgtacc acaatctata acatgggaac
    22321 aagagccaga aactgttaag gtgtggataa agcaacgtac tagatgggca aaagggaata
    22381 tatatgtatt aatgaaatat ataaaaaata tatttaaaca gggaagaaat aagattgtgt
    22441 ttgatatagc ttactttttt tctgtatatt ttctattttt aacatctgta attatatccg
    22501 atattttatt tgttttaagt atatctaaac tagtagaaat ttctattcca attaactttt
    22561 ttctaatatg gatactatct tatcttttat ttattataga agtgagtata agccttacta
    22621 tagaaaaagg tgaagcaaca attgaaaata tattcatagt tgcaataatg tattttacat
    22681 actctcaact atggctgttt gtagctataa aaggaatgat agagtatcta aaagatataa
    22741 tttttaaaag agaggttaaa tggtataaaa cagaaagatt ttgattcatg tacaggagga
    22801 attaacatga aaaaatttat tatatcaatt atatctttag tattgttttt ttcaaatata
    22861 tcacttatat acaaagtaaa tgctgatgag actaaagtta aaaattataa gtttgaaaga
    22921 gatataacta ttgatggtgt tataggaagt aatagtactt tttttgaagt aaataaaaat
    22981 tgggatattg aagaagtttt attgcatctt aattttagta agagccaaat attaaatgga
    23041 gatgtttcaa gtttaactgt attgataaat aatgtaccaa taaaatctat taaattaaat
    23101 gctaagacaa attataaaaa tacattagaa gtattagttc caaaagatta tataattcaa
    23161 ggatataatg aaataaaaat aaaaacatac aaaacaatat ctgataaaat ttgccaagat
    23221 gattccaata ctgggaactg gatggttatt cacaaagaat catatatatc aattagatat
    23281 aagcagaaaa aggtggaaaa ctcaataaat gaatatccgt atccatatgc agaaattgaa
    23341 aataatcata aattagatac aactatagta gtaccagata atatgactag aggagagact
    23401 acagcagtgt ttaatttggc atcagcattt ggaaaaataa caaaaaatga tgatttaaag
    23461 ttagatgtta aattatattc agaaatgaaa aattggtcag atgataatat tatatatatt
    23521 ggtaaacctg aaaatacagc agaagaaatt ttggatattt taagtataaa ggaacaaacc
    23581 ctattatctt caaactgtat aatcaagcaa gtagattcac catacaataa gaataagaag
    23641 atgatggtgg taattggtag taatgaagat gatttaataa aggcatcaaa tttattaata
    23701 gaaaatagac tttctaacca agttttatca tcatcagtac ttgtaaacaa agagactaat
    23761 ataaaaatta atagggaaca aaaattaaat ttaggtcatt taacattaaa agacttagga
    23821 tattctgact ttttgttgga aggagcattt aatcaacaag ctttgtttga tgtcaaaata
    23881 ccaacgggta aggtattaga tgatggttca aagattatct taaatttaag atattctgac
    23941 aatttagatt ttgaaaaatc tttagttaca gtgtctataa atgatgttat tgtgggaagt
    24001 aaaaaattgg ataggtcaca ttctaataat gataagttag aattaaaaat acctaaagat
    24061 atagataata aaaactatta tcaagttaaa ttgacattta atttgagtat aaaaaattcg
    24121 aattgtgtta ctagagagag taataatcct tgggcatatg tatcaaataa ttcttatttg
    24181 gcgttatcta cgaaagaaaa tgaaacctta tcttttgaaa attatccgta tccatttgtt
    24241 agagatgatg aatttaatga tttaactgtg ataatgccag attattcagg gtcacaagct
    24301 atgacctgga tgtttagatt aggtgttact ttaggtgcga atataaattc acataatgga
    24361 aatataaatg taattagagg aaaagaattt agtgacaagt ataaggatac taatattgta
    24421 gtttttggag tgccacataa taattctgtt ataaagatgt taaataataa tttaaatata
    24481 aagttcgata aaaattactc caattttata tcaaatgata agataagctt tattgatgat
    24541 tatggaaaaa atatttctac cattcaatta ataaaatcac catataataa tcaaaaaaat
    24601 atcatggtaa ttagttcaat aaatgaaaaa aacttatatt taggaatgga ttatttgttg
    24661 aataaaagta aagttaatga tttaaaagga gatacattga taattgatga atacggagag
    24721 gtagaagatt tagcttataa tttaaaatct aagaaagaag taaaagattc aagttggaat
    24781 atgagtataa ataagacaac taaagtattt ttaatgattt cttttattac tataattgta
    24841 gtcatgatat tatcaatgct atatataaaa aaatataaaa gaagataata tgataaattt
    24901 attattttaa aagaggtata taatttgagg aaatatatga ataaatttaa attaaaaaga
    24961 agtattttaa tctcattaat aatcttaatt tttatattta tagtttactt tgtaagtatt
    25021 gagttaaaag ataggagtct agtaaataat caatataaag gtaagattaa atcagccaat
    25081 ttatctgtag attatgaaat aaatcaagtt atgaaggata tagataaatt gggattgaat
    25141 acagtaaatg tacctataat tataaatgta gagtccatta attctgatat tatgtcaata
    25201 gataataata gtaaagaaaa ggcaattaag ttaataaaaa aattaaataa aaaaggtatt
    25261 tcaacaatat tagaggcata tccatggatt gaaaatggaa aattgtatga gactgactgg
    25321 aaccctataa ataaaaaaag atttttttat acttggcaaa atgccatatt aaatgaactt
    25381 ataattgatg tagcaaatcc actggatgta aatgttttaa atattggttc taattttgta
    25441 catttggaag aatatcaaca aaattggggt gaaataatag attttgtaca atccaaattt
    25501 gacggtcttg taacgtacag aactaattgg tggtatacaa aaaaagatga tttaagtagt
    25561 aagttgtttt atgaacaaaa gttgaacaat aatttttttg ataagttaga ttttatatct
    25621 atagcagcat attttgagtt aagcaataaa cctgttaata cagtggatga attaatttct
    25681 gccttacact catctactgt aaacaataga ggacaaaata taaaacaaga aatatataac
    25741 ctttataaag ctcatagaaa acctatattt tttggagaat taggtttttc aaatagagaa
    25801 agtgcaagtt ctcagccgtg gaatcatact ccttcaaaag ttgtaaatgg cgaagaacaa
    25861 gcaaggtgtt ttgaggcata taagaaagtt tttgaaaatg aagactggat aaatggtttt
    25921 tctgtgtttt gtgtaggtaa aatagatgac gaaaaaaatt tttacccatc aaaagagagt
    25981 ataaaagtta ttaagtcttg gtatgaataa ttttgaaagt gtagaattta tatagattaa
    26041 atagatgatt aggagagata taaatgaatg acatcaaggt tatgactgta tttggaacta
    26101 gacctgaagc tatcaaagta gcacctttaa taaaggaact tgaaaaaaga gagaatataa
    26161 aaagtattgt ttgcgttaca gctcaacata gagaaatgct tgaccaagtt atagaaacct
    26221 ttaatataaa tgtagactat gatttggata taatggaaaa aggacaaagc ctgaatgata
    26281 taacatgtaa gattttaaat aaacttccct tgattttaaa caaggaaaat ccaaatataa
    26341 tattggtaca tggagataca accacaactc tagctacaag tttgactgca ttttataata
    26401 aaactcttgt tggtcatata gaagctgggc ttagaaccta tgataaatat tctccttttc
    26461 cagaggagtt aaatagacaa cttacaggta ttattgctga tatgcacttt gctcctacta
    26521 atttggctag aaaaaacttg atttctgaag gaaaacctaa taataatata tttgtaactg
    26581 gtaatactgc tattgatgct ttaaagatga caataaaaga aaactataat catcctataa
    26641 ttgatgagat tggtaacgat aggatgattt tgttaacctc tcacagaaga gaaaatttgg
    26701 gtaaacctat gaaaaatata ttcagagcta taaagcgaat agtagatgac tttgaagatg
    26761 tacagatagt atatcccata catttaaatc ctaaaatacg aactatagct gatgagatat
    26821 ttggaaaatt tcctgaaaaa attcatatta tagagccttt agatgtagct gattttcata
    26881 actttttaaa taaatcttat atgataatga ctgatagtgg aggaatacag gaagaagctc
    26941 catcattagg taagccagtt cttgtgttgc gtgataaaac agaaagaaca gaaggaattg
    27001 aagcaaagac acttaaatta gtaggtacta atgaagatag aatatataat tcagtgagcg
    27061 atttattaat aaataaagat aactatgttc agatgagtaa agcatctaat ccatatggag
    27121 atggaaatgc aagcaaatat atagtggata taataattaa aaaatttaat tgcaaatatt
    27181 taaattagat aagggctaaa acattaacta attgatataa gatttatgat aatattaagg
    27241 aatacaattt ttaaggggga gtaatatggc tagaaaatta ataaaaaatt tgggtaaaag
    27301 taaaagtgta aagagagtga aacttttatt taagaagatt tttattactg tattcatagt
    27361 agcaagtata gttgctattt ttaatataac gaaatatttt gaggaattat acaaagtaag
    27421 ggatttaaaa agtactaaaa ttgaatacta tatggatgtg gctgatgaag caggagatgg
    27481 aaaggtccaa ttaagttgga aggccttact tgctatagac atggtaatac atgatgaaga
    27541 tttaagtaat ataaaaaaga aagacacatt ggatataggg gaaaagttta tagtagaaga
    27601 taaaaatgat aaaggcgaaa aggtgtataa ggtaaaaaag tttaataagg tattaagcga
    27661 attgaaattt gactcttctc aaaaaagtag agcaagaaaa tacatgaaag atttagaata
    27721 cacatacctt ggaaataaac aattagatag tagtgatgaa aaaattaaat ttataaagaa
    27781 gttagaagac tcagctataa gagaatatat tgattatgga atattgccct ctataacaat
    27841 tggacaagct atattagaat ctggttgggg aaattctaaa cttacaaaac agagtaataa
    27901 tttatttggt ataaaagcag ataaagcatg gaaaggaaaa agtgtagaaa tttcaacttc
    27961 agagcattat aatgaaaaaa ttgtagctag ttttaggtcg tataattcat tacaagattc
    28021 tgtcaaagac cacagtttat ttttaattaa taataaaaga tatagaaaac atgggttgtt
    28081 tgaggcaaaa gattatatta gtcaagctca atcattagaa aatgcaggat atagtacagc
    28141 agaagataaa aaaggaaatc gtatatatgc agagctatta atagatgtta ttaggagtta
    28201 taatttgcaa ttgatagata ataaagttga gacaaaataa ttgttaactt tatatgagga
    28261 gaaaacaaga taagattctt ttctacaaaa agaatagaaa aattgtaaaa aaaactaaaa
    28321 aaatgtattg acttgttatt taaaaggtga tattattatt actgtactcg aaagagaaca
    28381 aaa
//
LOCUS       DAFKMX010000028        28356 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFKMX010000028 DAFKMX010000000
VERSION     DAFKMX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.028"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            87..251
                     /locus_tag="LZB08_001034"
                     /pseudo
     CDS             87..251
                     /locus_tag="LZB08_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellobiose-phosphate degrading protein"
     gene            894..1619
                     /locus_tag="LZB08_001035"
     CDS             894..1619
                     /locus_tag="LZB08_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7314548.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNENKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            1749..3674
                     /gene="mngA"
                     /locus_tag="LZB08_001036"
     CDS             1749..3674
                     /gene="mngA"
                     /locus_tag="LZB08_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBM7314549.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIEDWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDSIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            3818..6433
                     /gene="mngB"
                     /locus_tag="LZB08_001037"
     CDS             3818..6433
                     /gene="mngB"
                     /locus_tag="LZB08_001037"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBM7314550.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            6712..7866
                     /locus_tag="LZB08_001038"
     CDS             6712..7866
                     /locus_tag="LZB08_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBM7314551.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7989..9902)
                     /locus_tag="LZB08_001039"
     CDS             complement(7989..9902)
                     /locus_tag="LZB08_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7314552.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFTQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            10144..10461
                     /locus_tag="LZB08_001040"
     CDS             10144..10461
                     /locus_tag="LZB08_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314553.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            10469..11716
                     /locus_tag="LZB08_001041"
     CDS             10469..11716
                     /locus_tag="LZB08_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7314554.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            11745..12098
                     /locus_tag="LZB08_001042"
     CDS             11745..12098
                     /locus_tag="LZB08_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7314555.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            12120..13565
                     /locus_tag="LZB08_001043"
     CDS             12120..13565
                     /locus_tag="LZB08_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBM7314556.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            13664..14281
                     /locus_tag="LZB08_001044"
     CDS             13664..14281
                     /locus_tag="LZB08_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314557.1"
                     /translation="MNVIKKFMFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADVI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSNI"
     gene            14577..15329
                     /locus_tag="LZB08_001045"
     CDS             14577..15329
                     /locus_tag="LZB08_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7314558.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            15431..16351
                     /locus_tag="LZB08_001046"
     CDS             15431..16351
                     /locus_tag="LZB08_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBM7314559.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            16354..18270
                     /locus_tag="LZB08_001047"
     CDS             16354..18270
                     /locus_tag="LZB08_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBM7314560.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGSLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAAIDNGDNLHLKTLSQLSSKLMNEEFLNELLNSETSR
                     EIVSKLNNEEIKSV"
     gene            18290..19159
                     /gene="gatY"
                     /locus_tag="LZB08_001048"
     CDS             18290..19159
                     /gene="gatY"
                     /locus_tag="LZB08_001048"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBM7314561.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            19669..21132
                     /locus_tag="LZB08_001049"
     CDS             19669..21132
                     /locus_tag="LZB08_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBM7314562.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            21136..22236
                     /locus_tag="LZB08_001050"
     CDS             21136..22236
                     /locus_tag="LZB08_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBM7314563.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            22269..22577
                     /locus_tag="LZB08_001051"
     CDS             22269..22577
                     /locus_tag="LZB08_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314564.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            22610..22990
                     /locus_tag="LZB08_001052"
     CDS             22610..22990
                     /locus_tag="LZB08_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBM7314565.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            22980..23408
                     /locus_tag="LZB08_001053"
     CDS             22980..23408
                     /locus_tag="LZB08_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314566.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            23457..26792
                     /locus_tag="LZB08_001054"
     CDS             23457..26792
                     /locus_tag="LZB08_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBM7314567.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLYGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            27173..>28356
                     /locus_tag="LZB08_001055"
     CDS             27173..>28356
                     /locus_tag="LZB08_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBM7314568.1"
                     /translation="MKAGEMEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     IL"
BASE COUNT        11191 a         2932 c         5217 g         9016 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagggtta ctcatccagg atttatagat tatagtctta
      121 taaaatcatc ttctcttatt caagagagag tatatgaaca tgcaatgcta tgtgataagg
      181 aagttaaaat atggttaaga agaaataata ttgagttggt tgattttaga gaccttttaa
      241 agagctttta aaaactaaca attaaatcta aaggtcatgt tgattttcta tcaacatata
      301 aggttttata ttaactaaat attgtgtaaa tcaaaataaa atagcaagat atataattag
      361 tttgctgaca aaattttagg atttttttga caaatgcatc ttctgtaaat gaatatagga
      421 tgtagaacct ttgtaaaatt aggctttaca tccttgttaa taacataaat atattagaat
      481 acgaacaaca tccgtatttg tgctaggata atcagcagta ccatctgaaa tagaattttg
      541 atttagcaca caatgggcaa caaatataac tcgtttgctt cttatatctg taaacatact
      601 cttttcacct cacttatata gaattaagtt atgatttatc aagttgcgat aaaaattaat
      661 ctatcttaaa ttctaatatc tgttaatgat aatgtcaaat cgattatatt caaaagataa
      721 ctaagtatat taaacaacac acttttaaat tatctccatc aatataggaa gtacttattt
      781 tttatgtata ataaaaggta taatatatgt gaaaaaatta aaaatattac taaaaaattt
      841 agataataca gagtattata tttatgaata ataaaataga tggaggagaa actatggaat
      901 tttatgaaag agcaaaaaaa tatgagtata aatttacaga tacagaagac atgataattg
      961 aatatatgag aaaaaacaaa gaagaaatag taaaattatc aatacaatct ttagcagata
     1021 aattttttac tgtcccaaat accatagtta ggctatgtaa aaaaatggag tatgatggtt
     1081 tctctcaact aaaaaataat ttaaaagaag agttaaaaaa tgagaataaa gagccaaaaa
     1141 atggttttga atcagatata aaaaagacat tagagctaat agattatgaa aaaataaata
     1201 aagtagcaga tataattcaa aaatctagga gtattatagt ttatggaata ggtcaaaaca
     1261 ttcctttatg tgagatatta gtaaaagaaa ttagaggtgg gcatgataat ataacttact
     1321 atagtcaaag acatgaagta cttcatgata taggaaaatt aggtgaacaa gatatgtttt
     1381 tcgcacttag tttaagtgga gaaacgaaag aagttataga agcagcaaag acagcagtta
     1441 ataagggttg taaggtagta gctctaacac atatatgtga aaatcaattg caacaaattg
     1501 cagatataag tttatattgc tattcaacaa ttaaacaagt aaatgggtac aatatatcag
     1561 ataaggttcc aattatgatg attatgagag caataataga gcaatactgg ttaagataga
     1621 gatttgtgta taaatacaca aatctttatt ttttaagaag aaattgtgtg tataatttca
     1681 aaaaaacatt ttcataattt attttatgct atattcatag cattaaatga tttaaggagg
     1741 gcgtaatttt ggaatttaag aaaatcacaa atgaaaacct tgttttttta aaacaaaaaa
     1801 taaaaacaaa gaaagacgca ataaagttct tagcaagaaa gctatatgat gaaggcaagt
     1861 taacatctta tgaggaatat ttagaagctg taatgtacag agaaacttta tctccaacag
     1921 gttttgaaag agggcttgca attcctcatg gaaaatctaa gtcagttaaa gaagctgcat
     1981 ttgcagctat gaccttagag acaccaatag aagattggga aagcgtagac ccaaataata
     2041 aagtagagat aatattttta atagcagtac cagaaaatga aggtggaaat acacacatac
     2101 aaatactttc tgagttagtt actagactgt caaatgaaga ttatatgaat atgttgttaa
     2161 attcaaaaac taagaaagag ttatttaata acttagatat aaaagaaaag aaagaacaaa
     2221 taaaagaaga gattaggatt caaaatgaat caaaaaaaat tatattagca ataacagcat
     2281 gccctgcagg tatagcacat acatatatgt cagcagaagc attaattaaa gctggtaaag
     2341 aaatcggtgt ggatgtgtat gtagaaaagc aaggagctaa tggtatggtg gaccctcata
     2401 caccagatat aataaaaagg gcagatgcaa taatatatgc aacagatgta gcacctaaaa
     2461 atacagaaag atttgaacat ttgccaaaca taaaaacgtc agtagcagct ccactaaaaa
     2521 gagcaaaaga aataatatac gaagctttag aagtagcaca aaaacaaggt aaaggtgatt
     2581 atatagaaaa atcatctgat atatcatatt gtgaaaaatc atcatggaaa aaagatgtaa
     2641 aatcagcaat acttacagga gtatctcata taataccact tattgtggca ggaggtatga
     2701 tacttgcatt tgcagtatta gcatctcaag catttggtct acaagaacta tacaatcaag
     2761 aaggttcttg gttatggcaa ttaagaaagc tttctggagg tatgttaggt acattgatgg
     2821 ttccagtatt atcagcatat atgtcttact cactagctga aaaaccaggt cttggaccag
     2881 gatttgcagc aggtttagca gctgatttaa taggtagtgg atttcttgga ggtatgttag
     2941 gaggtttact tgctggttat atagtaaaat ttttaaagaa gaatatacca gccaaaggca
     3001 cattagctgg attcgtaagt ttttgggtat atccagtagt tggttctttg gtagtaggtt
     3061 tattgatgtt atttgtagta ggaaagcctg tagcagcact taacaatggt cttataaatt
     3121 ggttaaatgg gatgactgga gtaaatgcaa tactactagg tgcaataata ggagctatgg
     3181 tttcatttga cttaggtgga ccaataaata aagcagccta tacatttgca ataggagcaa
     3241 tagcaagtgg aaattttata ccttatgcaa tatttgcatc agttaagatg gtttcagctt
     3301 ttggagttac aatagctact gttttaggca gagatttatt ttcagaatca gaaaaggaaa
     3361 taggaaagca aacttggata ttaggattgg ctggaataac tgaaggtgca ataccattta
     3421 tgatagagga ctcaatccgt gttataccat cattggtagc tggttcagcc ataactgggt
     3481 ctatagtagc attcttcaat ataggtcttc aagtgcctgg agcggggata ttctcattgg
     3541 cgcttttaga gggaagtatg tcaggattta ttatagcatc aatatggttc tttgcggcag
     3601 tacttggagc tataatatca gcaatattat taataataac taggaaacaa aaaaatcaaa
     3661 gaaagataca ttaaaagata cgttattaga tgtttaaatc aaaggtttaa ttatagtgat
     3721 aaaataaata ttaaaaaatt ttgtaaactc agtagtattt ttattggatt tttaattaga
     3781 cagaaaatat tatcagataa gttttggagg ataagcaatg aaaaaaaatg tacacatagt
     3841 tcctcatatg cattgggata gagaatggta cttctcaact gaggaatcta gaatattact
     3901 tgtaaataat atggaagaaa taatggatat gttagaaaat aatcataact atccatatta
     3961 tgtgatggat ggtcaaactg ctatattaga agattattta gcagttaagc ctgaatgtaa
     4021 ggagagaata aaaaaattag ttcaagaagg aaggctaata ataggtcctt ggtatactca
     4081 aactgatgaa atggtagttg gtggagaatc catacttaga aatttactat atggaataaa
     4141 agattgtgat gaatttggag aatatatgaa aataggatat ttaccagatt catttggtca
     4201 aagtgctcaa atgccacaaa tactaaatgg gtttgatata aaatactcaa tgttttggag
     4261 aggatgttct gaaagaaaag gaactaataa aacagagttt aattggaaga gtgatgatgg
     4321 gtcttctgtt ttggttcaaa tacttccatt aggttatgca ataggaaaat atctaccaat
     4381 gaatgaagat gagctaagaa caagaatgga taaatattta ccggttcttg ataagggtgc
     4441 aactactgac catataatac ttccaaatgg acatgaccaa atgccaattc aaaagaatat
     4501 atttgaagtt atatataaat taaaagaatg ttatccagag agaaaatttt tcttaagtag
     4561 atatgaaaac atatttaaag agctagaaaa aaatgaggat atagatacaa taaaagggga
     4621 atttttagat gggaaatata tgagggttca tagaagtata ttctcaacaa gaatggatat
     4681 aaaatcagca aatgctagaa tagaatcaaa gataaccaac atattggagc cactagcttc
     4741 tattgcatat tctctaggat ttgaatatca tcatggatta atagagttaa tttggaaaga
     4801 aataatgaaa aatcatgctc atgacagcat tggatgttgt tgttcagata aagttcacca
     4861 tgaaataatg aatagatttt tcttagctga ggaaaaagta gaccaattaa taacatttta
     4921 caaaagaaga atagttgatg caatgagttg tgaaatggct ttagataagt tagtagcatt
     4981 taacttaatg ccttacgata ggaatgaaat agtaagcgcc caagttataa ctaaaatgaa
     5041 agcatttgaa atatttgata aagataataa taagttggat tttgaagtgg tacataaaga
     5101 agtaatagat gcaggtttaa tagaccgtca aattgttcat tatggaaact atgattcatt
     5161 tatggtatat acaataaact ttaaagataa aatacctgca atggggtata aggcgtatat
     5221 gataaaagaa gttgaacata tgttagaaaa agcttatgaa gtatgtgaca tggtagataa
     5281 tgacttttat actattcaag ttaatgaaaa cgggacatta aagatatttg ataaaaaatt
     5341 aaataaaact tttgaaaatg tattattgat ggaaaatgga ggagatgaag gagatgagta
     5401 tgatttttca ccacttccaa atgagaaatt aatatttaac gataatatag tagcaaattc
     5461 cactataaag aaaaatagat ttaataatga gataaagata aattatagat taaaagtacc
     5521 aaaagatata caagctagaa aaaataatag agcagattca tttattgact ttgatatagt
     5581 tataaatgtt ccaaatgata aagctataat agatgtagta ttcaacataa ataaccaagc
     5641 aagagattgt agggttagaa cttatatacc aacaaatata gcttcaaagt tttctgtgtc
     5701 tgataatcag tttggacata taaagagaag tgtgtatgat gatgctatgg aagtttggca
     5761 acaggaagac tggagtgaaa gaccagattc tatatatcca atgctaacat tcgttggatt
     5821 gtctgacaaa gagcatggag tagctgttct tacaaacagt acaagagagt ttgaaatagt
     5881 agggaaaaaa tttgacacaa tagctataac attatttaga agtgtaggat tcctaggtaa
     5941 agaagaaatg gcaagacgcc caggtcgtcc atcaggaata aagttgccta ctccagattc
     6001 tcaaatgata ggagatataa aaatagactt agcaatagtt actcatgaaa aatctacact
     6061 agaagcaaat gttgcaaata tggctaaaga gtatttaaca aaaatacaaa cttataataa
     6121 aatgccatat aatgctatga aattgaatca atcagaaata gaagtagatt attcatatag
     6181 tttacttaag gaaactaata aggaccttgt gttaagtgtt gtaaaaaagg ctgaaaaaga
     6241 agaggcatta gtagttagat tttataattc taaagaagaa acaaaatcag ctaacatagt
     6301 atttaacaaa aatattaaaa cagctaaatg gattaaccta aatgaaaaag aattagaatc
     6361 tatatcaata gataataact ctgtaaatgt agagtgctta atgaaccaag ttaaaactat
     6421 actggttaaa tagtgctaaa aatgattaga tagtgctaaa aaggaattat cttaaaatag
     6481 agataatcct ccttatcatt gttgaaataa taaacatata tttgatttta tacaaaagag
     6541 tgtctcatga actaagaaat ttattttgag acactccctt tttataagat aaaagaatat
     6601 taatggtata agttgtatac aaagagagat gtagagtata aaataaaaga taaattaagc
     6661 ttataaaatt atatgtttaa aataaggagt aataaaaagg agtagataag tatgaaaata
     6721 ttaatttcta tagattcttt gaaaggaagc ttatcttcaa tagaggcagg aaatgcaata
     6781 aaaaagggga tattgaaggt taaagaagat gcgcaagtaa agatattgcc acttgctgat
     6841 ggtggtgaag ggacagtaga tgctttagtt caagggatga atggtaaaaa agagactata
     6901 gaagttactg gaccaatagc taaaaaagtg gatgcaacat atggattatt aaaaaataca
     6961 agtacagcaa taatagaaat agctcaagca tcaggattaa cattggttcc gactgaactt
     7021 agaaatcctt tatacactac aacttatggt gttggggaaa taataaagga agcaataaat
     7081 aaaggctata gaaattttat agttgggata ggtggaagtg ctactaatga tgctgggata
     7141 ggtatgcttc aagccttagg gtttgagttt tatgataaaa ataacaagtt agtaggatta
     7201 ggtggaaaag ttttaaatga aataagacat ataaaaatag aaaatagatt aaaggaatta
     7261 gatgaatgta agtttaaaat agcctgtgat gtaaataatc cgctgtttgg gaaaaatgga
     7321 gctgcataca tatacgggtc tcaaaaaggt gcaacatcag aaataattga ggagttagat
     7381 aatgggctta gaaatttttc caaagtagtt aaaaattact taagtaaaga tgtagctaat
     7441 gtagaaggag caggagcagc aggaggatta ggttttgctt ttttagcatt tttagattca
     7501 aaattagaat ctggaataaa aataatatta gaagaaatta aattagagga agagttaaaa
     7561 gatgcagatt ttgtcataac tggtgaaggt agattggaca atcaaactgc tatgggcaag
     7621 gctccaatag gagttgcaaa actggcaaaa aaatatgggg taaaagtaat aggtttggct
     7681 ggagccacaa cagaagatgc agttaaatgt aatgaagagg gtatagatgc ttatttttct
     7741 atagtaaata gagctatgac aatagaagaa gcaatggata aagcaactgc tagtgagaat
     7801 atgacagcta caacaacaca aatattcaat cttataacta gtatacaaca gtcaaaaaat
     7861 atgtagtatt aaaacaaagt atattaggta aaaagctgtc acatattaat ttagactttt
     7921 tagtgtgaca gcttatctat tttatgttaa taacttaaga aacatatatt ggaaataaat
     7981 taattgattt agatattttg aataagataa gctattctat tcataaaact actatagtta
     8041 tctgccttag taagttcttt aacattattc cactcagata atatttcaat aaagctttca
     8101 aatatgtaat tgaatttttc tttttctttt tcatttatag gtatcatagc tataatttga
     8161 actttttcct caccccattt aactggttta tcgttaacaa ttaaacatac tccagttttt
     8221 aaagcatcca ttttcatgct atgaggaatt gctacattgt tattaaatgc agttgaagac
     8281 attttttctc ttataagaac atcatctata taattgggtt tgacatattt attttcgtac
     8341 agaatatttc ccataaattt tatataatct ttagcactat ctaaatatat atttttcata
     8401 aacaaagatt cactaaagta catttctaaa tgattttgta cagtaagtat attttttcta
     8461 tctttaattt gagtaaattt actttgaata ttatcataat cctttctatt taggtaagga
     8521 tttacataaa caaaaggtat agggcatcta ttgttaattg gtaaagtagt aataaccatg
     8581 tctaccttat tgtcaaaatc aagattatga gtatctgaaa aaatctgaat aatttctatg
     8641 gattcattaa atctcttctc aaggtctctt actattttga attgtaaatc atagtaattt
     8701 ggagtaatta atgcacatag aactttatct tctaataatt tttgtctatc aaagaatgaa
     8761 cctacatgaa gagatatata agatatttca tcttcgtcta tatctacatt ttctaaagtt
     8821 tgaatctgat tgcaaatata aacagatata tcatatatca aagggtagga atcttttaac
     8881 ttttgaggta tttgatttct taaaacttga ttatttttgg ctctactgat taaatttttt
     8941 acatgaagag taaattttac aacaaattcc tcatcagata aatctataag atataagtca
     9001 tatacatttt taattatttt tttagttaat ttaacaaagt gctcatctat ataatttgac
     9061 aatgatttag tatccatttg attgtagttt aagactgtag ttttgtttgt taactgaaat
     9121 actaaatagt ataattccgc actagttaaa gttatattgt attcagattc taaaaaattt
     9181 gatatgtctt ttgcaatatt tatatctacg ttattgtcta tgtagtttac tacttcaaca
     9241 gagtctatgt aattattctt ttttattctg tctatagtta tcattaaatg aagaagtatg
     9301 ttgtttatag catactcatt tataaataaa ttgtattttt ttaatatata tattatattt
     9361 tcttttagtt tagtcaaatt atactcttga tatatttttt gtacatcaaa taagcttaaa
     9421 aaatcacttc ccgtttcttt aaatattata gagctcatta acttgcgctt tgcagattca
     9481 tttccaatta gtttcaataa aaatttcgat tgctttatct ttaaattata acttcctata
     9541 aatttattag ctttgactat atccccttca atagtagctg ggctaacaaa caattcttca
     9601 cttaaatcaa atatattaat tccattacta ttaactataa gttcttttaa aattaaattt
     9661 aacctgttat caggtgatat attttctgaa ctatcaacct tggtttgaca attattattg
     9721 agatttactt tatatccttt tttagaggat aatataaatt caccattgga attaatttcg
     9781 tttacatatt ttctaattga acgcgttgag acattaagat aattagccaa tgtagatgaa
     9841 tctatccagt catgttgtgt ttttatgaaa tttaaaaata gttctttttt agatgttttc
     9901 attagattcc ctcgcatagt tttaaaataa aatctggatg atatgataat tatataattg
     9961 aatgtacgta ttataagata cattatacta tattaatatg tttaattaag agtaactgat
    10021 ttcctattat taggaaatat atttatgtga aatgaactta tttggtaagt acaatataaa
    10081 cataataaaa taaaaagttc attaagaaat aaattaatgg ataaaaatat tggaggtatc
    10141 attatgaaaa atataagagt gcttttggcg tgtggttcag gagcaagtac aggttttatg
    10201 gcaactagta tgagaaagag tgcaaagaaa aaaggtatag aagcagatat aaaagcgaga
    10261 agtgagtcag agatagataa gtatttatca gaaatagatt gtttattact aggaccacat
    10321 ttagattata tgaaggaaga aattcaacaa aaggctaatg aatataacgt tccagtagca
    10381 tgtatatcta aaactgcata tggaagacta gatggtgcaa aggctttgga agaagcacta
    10441 gaactaatat caaacaaata aaatatttat ggggagagtg tttatgaata aatttataga
    10501 ttttttaagt gaaaaattta ctcctgttgt aaataatatg actaagaata tatgggtaca
    10561 atcagttcaa tcaactataa tgaaagtttt gccaatggta tttgtaggct cattagtaac
    10621 tatagtttct gtcttgaaga attacataag ctttttacct gatttaagtc caataaatca
    10681 atatacattt ggacttttag gattatttat agcattttta ttgcctatgg aaataatgaa
    10741 gaataagaaa tttgaaagta tgagtgtagt agctggtctt gcaggtgctg gattaatgtt
    10801 aatgatgatt cgtccagaga ttacgaatga aggagctata tttaacttca acaggtttgg
    10861 aggagaagga atgcttgtat cgctagttgc aggattattc tctggattaa taatgtcttt
    10921 atttggttct ttttcatttt tcggagaaga ttcagcactt ccggattttg taagcaagtg
    10981 gtttgataat atgttaccta taacagtgat aatgtttgta ggctggggat taatttatgg
    11041 actcaacttt gacatgtttg aaataatagt tagagtattt aaaccagtat cagatatagc
    11101 acagacatta cctggatttg tactcttaaa cttcttagta gtatttatat attctttagg
    11161 aataagtggg tgggtactat ctcctataac ttattcaatt caacttggtg caattgcagc
    11221 taatgcagca gcagtagcat caggaggagt agcaactaat atatatgttt acgaagttat
    11281 tcaaacaggt tggataactt taggagggtt gggtgctaca ttaccattag ttataatgat
    11341 gtgtttatcc agagttggaa gaataaaagc aataggaaga gcatgtattg tcccttctat
    11401 aatgaatata aatgaaccag taatatttgg agctccagtg gcatggaacc catttttaat
    11461 gttacctatg tggataggaa cagtagtaat atcaataact acttacttat caatgagtat
    11521 gggatttgtg acaattccaa gtaaggtgtt taatttatgg tatgttcctt ttggtatatc
    11581 tacttttatg gtaaatcaag atgttagagg tcttattgtt ttagcaataa atatagtatt
    11641 gctatttatg atatggtatc cattttttaa agcttatgag aaagaagaga taaaattaga
    11701 gagccaagag gtttaaattt agaaattgaa aggaaattat aaatatgaca attgaagaac
    11761 atataataga tgaagaatta gtagaggtgg caatgcaaat aatacttcgt gcaggtgagg
    11821 ctagaacaga aattaaacat gctcttaatg atttggaaaa gtttgattat agaaatgctg
    11881 atttaaaact agctaaagca aaagggttta tgacagaagc acatagagct caaaccaaga
    11941 ttatacaagg ggaagctggt ggagagaaaa gagcacattc gctgttattt gcacatgctc
    12001 aagatactct tatgaccata ttcagcgagt taaatataac tatgtcttta gtaggaatag
    12061 taaagtctat agaatataag gtaaataata gtaaatgatt taaataaata aaataaggag
    12121 tgattaaatt gggtaagttt cctaaaggtt ttttatgggg aggagctgta gcagctaatc
    12181 aattagaagg tgcatggaat gaaggtggca aaggttggtg cttagctgat gtacatatgt
    12241 atgataaaga tagagatata tctaaacctt atgagtctga tatgactata gaaagaataa
    12301 agtttgctat gaaagataat aatggatatt atcctaagcg tagtggaata gacttttatc
    12361 atacatacaa agaagattta aagcttttga aagagctagg gttaaagtgt tttagatttt
    12421 caataaattg ggctagaata tttccaaatg gagatgattt agagcctaat gaagagggat
    12481 taaagtttta tgataatctt ataaacgaag taatagataa tggtatggaa cctattgtaa
    12541 cactacttca ctatgagact cctttgaatt tagtattaaa atataaagga tggtctaata
    12601 gaaaagtggt agatttcttt gttagatatg cagaattaat actaaataga tataaaaata
    12661 aagttaaata ttggatagtt ataaatcaaa taaatttaat acattatgaa tcatttaatt
    12721 cagttgcatt ttgtgtagac caagtagata atgtagaaga ggctagatat caagctattc
    12781 atcatcaatt catagctaca gctttgacta agaagcttgg aaaagaaata aattcagata
    12841 tgatgatagg tatgatgatg gcagattgta catcttatcc agaagattgt gaccctgaaa
    12901 atgtagtatt tgctttaaaa agaaataggc tacaatattt tttcactgat gtaagtttta
    12961 ggggagagta tccagtctat attaagagat ttttcaaaga aaataacata aatatagtta
    13021 tggaggaagg tgatgataaa atcttaaaag aaaatactat ggattttcta gctctatcat
    13081 actattactc tacaacagta tcagcaagta aaaacaccat gaatccaact gacaatataa
    13141 agaatccata tttaaaagca aatccttggg ggtgggcaat tgacccaaaa ggattataca
    13201 atactttatc tcaatattat gatagatacc aaaagccaat aatgatagct gaaaatggat
    13261 ttggaatgca tgataaattt gaagatggaa aagttcatga tagttacaga atagattatt
    13321 taggaaaaca tatagaacaa ataggtgaag ctatagctga tggaaccgaa atattagctt
    13381 attgtatgtg gtcaccaata gatatagtaa gttgttcatc tcaagaaatg gaaaaaagat
    13441 atggatttat atatgtagat atagataaca atggaaatgg aagtaaaaaa cgttataaaa
    13501 aagatagttt ttattggtat caaaatgtaa taagaactaa tggagaagaa ttaaaagaaa
    13561 agtaaatagt ttttaaatat aaataatccc tcttataaat actatgttat taatacagtt
    13621 ttataagagg gattataatt ataaataagg tggtttatta attatgaatg tcataaaaaa
    13681 atttatgttt gtaattttaa ttataaatat actgggatgt ggtatatctc ttatactcca
    13741 gtcaagcctt ggtagtgatg caattacact tttaaatgag ggtatacata taaagttagg
    13801 tataagttat acaatagcag ggctaatata taatggaact ctattactta tagcattgct
    13861 attgaataaa aaaagtttgg gattaggaag ctgtgtttat gtactagtag caggaatatt
    13921 tatagatttg tatatatact tattattaga ttttaatata agtagtttag ggattatcat
    13981 tagaataatg agctttataa ttggacatat acttatgtgt agtggatttg caatgcttat
    14041 aaaatttgac attggaatga gccctttaga tgctatatta atctatattg agggcaaatt
    14101 taagtatcca tataaagttt taaaaactat agcagatgta atatttttaa ttttaggtgt
    14161 atatttaggc ggaaatttag ggtttggaac tatattttca atattatgta ctggaactac
    14221 aatttcaatt gttagtaaga tgatttctgt taataaaaaa aggtattact caaatatata
    14281 aattgataag tgagagtatg gataatgttt atgaatcatt ttcaataatt cgagttaatt
    14341 tgaggtcgta aaaattggag ttactcagtt tcagtaattg agtaactcca atttttatta
    14401 caaaatactc taataattac ataattaatt gaagcataaa attattatat tataaattta
    14461 catatataga cattgactaa atcttaattg tatataaaaa taagtagtta agaggtaata
    14521 tattatatta ctcgataaat ttagacaaat aaaacttata taaggggaga taacatatga
    14581 taattgagct aaacaaaagt aattctgaaa atctaacgaa gacggaactt gaaataatta
    14641 aatttataaa tgaaaatgaa tcaatattac cagaattatc tatagtagag attgctcatg
    14701 aaacatattc atctccagca accgtttcaa gagcaattag aaaatgcggt ttaaatggat
    14761 tcaatgaact taggtataga ttaacagtaa aagatgaaaa tgatgatata caaaatatgg
    14821 gagatataat aaataaatcc tttatagaag cacagagagt tatagaacaa atatctttaa
    14881 ctacactaat caatataata aaaactataa ataaatctag taagatatat attttagcaa
    14941 gagggcttac tgaatatgta gcaaaagagt ttagtcttaa attgcaatta cttgatttta
    15001 atgttgtttt tataaatgac cccaacataa tgaaaataaa atctaaatca ataaaaaaag
    15061 acgaacttat aattatattt tcattgaatg gaaataccat agagcttgta gaatcagcac
    15121 agaatgccaa tttagttgga gctaaggtta taacttgctg ttgtagtgaa agtgcaaaac
    15181 ttataaagta ttcggattta tatattgtag gatacaaaca tgaacatata tcaatttctg
    15241 aatatgaagt atcttccaga ttgcccttgt atattatatc taggataatt atagattata
    15301 tggtcagata taataattct gaaaattaaa ttgaaaagtc tatgaaaatc gattttctat
    15361 ttgtgaaaag attttcatag acttttttat tataatgaaa acatacacag cacaagcaag
    15421 ggggaagaca atgatatata cattaactac aaatcctgca atagatatga atatatctac
    15481 taatgggata aagagaaaat tagtaaatcg tacatcaaat gctgtatatt ctcctaatgg
    15541 aaagggatta aatgtaactt ttgtactagg tcattatggt atagaatcta agatattagg
    15601 attttttgga ggattttcag gaaaatatat agttgaagaa agcgaaaaga gaggatttga
    15661 cgtattgcct acttgggtag aagatacaac tcgtataaat atatttttaa atgatggaag
    15721 tgatgaattt aaatttgtaa actctgggtc atatgtaaat gaaaaacaaa aactagatat
    15781 gctagataag atacagtcat tagcagatat aagttactta tcaataagtg gaagtctacc
    15841 tcctggtatt gatgataatt actatgagga tatatttaaa atttgtaaaa ataaaagtat
    15901 aaagacaata ctcgatataa gttcaccaaa attaaaggag ttactacagt ataatccata
    15961 tttgataaaa ccaaatgatg aagaaatcaa agatatattt ggaattattg taagagatga
    16021 agaagatatt aaggatgtac ttaaattact acatttaaaa ggtgctcaaa atatattgct
    16081 aactttagga gaaaaaggtt cttattttta taatggaaaa tcaatttatt atgctagtgc
    16141 tcaaccagtt acagtagtca gctctgcatg tgctggtgat tctgcattag ctgctttttt
    16201 aagtatatgg ttggaaaatc cagaacatat tgaagaagca ttaaaaaggt cagcagcaac
    16261 aggagcaagt gtggctgaaa gtaatggtat aggtaatttg gagaatgtgg aagattatat
    16321 aaaaaatata agagtcagaa aggtggtata aaaatgggaa agaaaatgat tctaggggta
    16381 acaggttgtc caactggaat agctcatact tttatggcag aagaagcatt aaaaaattca
    16441 gccagggaat tagggtgtga cataaaagtt gaaacgaatg gagctatagg tgtagaaaat
    16501 aagttaacag caaaagatat cgaaatggca gatgcaataa tagttgcttg tgataaaaat
    16561 gttgatatgg acagattcaa tggaaagcca gtaatagaag tacctgtcaa agaaggtata
    16621 cataaagcta atgaacttat acaaagatgt attgatggaa aagtagctat tagaaaagga
    16681 aacttatcaa gcagtaaaat tgcagaagaa ggaagtttat cgtttggtca aaagttgtat
    16741 aaagacttaa tgaatggtgt atctcacatg ttacctttag tagtagcagg tggagtactt
    16801 actgctatat catttttatg ggggatttat tcatttgacc caaattcaga acaatataat
    16861 caaatagcag ctacattaaa atctgttggt ggatattcta tgaacttgat ggttcctgta
    16921 ttggcagcat ttatagctca atctatatcg ggaagagctg gtatgttagc aggacttgtt
    16981 ggaggaatga tatcatttga tacaggttcg gggtttttag gtggaatcat atcaggattt
    17041 ttagcaggtt acttagttaa actatttgta catttgttaa gaaaattacc acgacaatta
    17101 gaaggtctta aatcgatttt tataattcct attgtcagcg taggtcttgt gggtgtagca
    17161 atgttgttac ttggtgggcc atgttcagca cttaacaatg ctatgatgaa ctttctatcg
    17221 gggttacaaa attcaagccc tataattttg ggaatagtaa taggatgtat gtcagcattt
    17281 gatatgggag gtccagtaaa taaagcagca tatgtaactg gaactatgct tctaggacag
    17341 ggaaattatt tatttatggc aggtgtatct gcagcttgta taacaccacc attggtaata
    17401 gcaatagcat ctactttatt taaaaataga tttacagaag aagatagagc agcaggatta
    17461 atcaattata tactaggtag tactcatata acagaggggg caataccttt tgcagcaaaa
    17521 aatccattaa aagtgttgcc agtacttatg attgcatcat caatatcatc aattttaact
    17581 tatataatga aaatagaagt accagctcca catggagggt ttttggttct aggattagtc
    17641 aataagccac ttttatgggt agggtgtata tttgtaggtt cattagtagg tgctgtactt
    17701 tatatgatgg ttacacctaa agtagtaaat gagaaaaata ctatagaaaa tacaaatgca
    17761 acaaaagatg aacataaaaa tgaaagtgca gaatcggaaa acgtatctaa taatatttct
    17821 ttatatagcg aagaaacagt aattttagat gttaagggaa aaaataaaac agatgtaatt
    17881 gatgaaatga ttggaatact tgataaatct ggtgttcttt tagataaaaa gaagtttaaa
    17941 gaagaaatcc ttaaaagaga agaaatatca tctacaggtt ttggtatggg gatagcgata
    18001 ccacatgcaa aaacagatgc agtaaaggta ccacgtgtag cagtaggtgt atcaaaagaa
    18061 gggtttgact ttgaatcaga agatggaagc ttggcacata ttatatttat gatagcagct
    18121 atagataatg gtgataattt acacttaaaa acattatctc aactatcttc aaaattaatg
    18181 aatgaagaat ttttaaatga gttattaaat agcgaaacaa gtagagaaat agtatcaaaa
    18241 ttaaataacg aagaaattaa atcagtataa acatatagga ggagaaatta tggcattagt
    18301 gacaactaaa caaatattat tagatgcaca agaagggcat tatgcagtag gtgcatttaa
    18361 tgttgaaaat atggagatgg ttatggcagt aatagaggct gctgaagaac ttaaatcacc
    18421 agtaatattg caaacaacac cttcaacaat aaagtatgca ggactagatt attatttagc
    18481 gaatgtaaag gtagcatcag agaaggcaag tgttccagtg gcaatgcact tagaccatgg
    18541 ttcaagcttt ggactagcta tgcaggcact tagagttgga tatacttcta ttatgattga
    18601 tggttctcat gaaagttatg aagataatat ttcaatatct aaatctgttg tagatgcatg
    18661 tatgccttca gatatacctg ttgaagctga attaggtaaa gttggaggta aggaagacga
    18721 tttagatggt ggtgatgggg gagcatatac agacccatta gaagcaaaag agtttgtaga
    18781 gagaacaggg gtatcatctt tagcagtagc aataggtacg gcacatggat tatataaggg
    18841 agagccaaag cttgatttag atagattatc tgaaattaga gatgttgtat cagttccatt
    18901 agtattacat ggtgggtctg gaataccaga tgaagttatt aaggaatcta taaaaagagg
    18961 gatttgcaag gttaattatg caacagaatt aaggattgct tatagcaatg gggttaagga
    19021 tgtgcttaat tctgacccag aggtaattga ccctaagaaa tatggtaaaa aaggtttaga
    19081 gtcagttaaa gattttgtta agagtaggat ggaagtttgt ggatgtgtag gaaaagcaat
    19141 agagatagtg aagtgttaat aataaaggga gagaataagt aagattaaat taaggggtgt
    19201 ctcaaaatga actttttagt tcaggaggca ctcttttttg tatgaaatta aatatatttt
    19261 catcattccg aaaataaaaa agaagattat atctattttg agacagcatc ttattttatt
    19321 ttttgattaa gattgtaatt gagaaatttg aaataaggaa gatgatataa ttaaataaaa
    19381 aatttctttc taagtttaaa ttaatattaa aaagttataa tatggtaaga gttatccagg
    19441 tagatgactt ctatgatgga tatatgataa aataattttg tatggtatat ataataatca
    19501 taaaataaga gtattgcatt agttttttaa ctaaaacatg taaataggaa aaataattta
    19561 ataactgtgt agtaaaaact tggatttaac ataagacact aactattaag gcgtacgact
    19621 aaaatatata tttaaattat tttatgaaca ctggagggaa agtcatatat gattacagga
    19681 gaattaagga gcaaagtaga caagatttgg gagacatttt ggactggtgg attaactaat
    19741 ccattagaag ttatagaaca atttacatat cttttattta ttaaaggatt ggatgaagta
    19801 gaaacaaaaa atgaagcaga agcaactctt ctgggagttt cttttgaaag gatatttgat
    19861 gatgaacatc aacatttaag atggagtaag ttttctaatg aaggaaattc agaaaaaatg
    19921 tatgaaatag tacaaaatga agtgtttcct ttcataaaaa aattacatgg aaataaagac
    19981 tcagcttatg caaagtacat gggagatgca atatttaaaa ttcctacgcc tcttatgtta
    20041 tctaaaatag ttgatggtat tagtaattta gaattaagta aggatagaga tacaaaggga
    20101 gatttgtatg agtatttatt atccaaagtg gctacagcag gtactaatgg tcaatttagg
    20161 actccaagac acataataga tatgatagta agacttatta aaccaacacc agaagatatt
    20221 atagtagacc cggcagcagg ttcagcaggt tttttagtat catcacaaca atacttaagg
    20281 gataatcatt caagtctttt cttagtgcaa gggctgaagg agcattttaa taatggtatg
    20341 ttctatggat ttgatatgga tagaactatg cttagaatag gtgctatgaa tatgatgttg
    20401 catggcgttg acaatcctaa tatagagtat aaggattctt tatcggaggt taatacagat
    20461 aaagataagt ttacattggt gttagcaaat cctcctttta agggaagttt agattatgaa
    20521 gcagtgtcag cagatttatt aaaagtgact aagacaaaga aaacagagtt attattcttg
    20581 gcactttttt taagagtttt aaaaattggt ggaagatgcg catgtatagt gccagatgga
    20641 gtattgtttg gttcaacagg tggacataaa tcgataagaa aagaaatagt tgataatcat
    20701 aaattggaag ctataatatc aatgccaagt ggagtgttta agccttatgc aggagtatct
    20761 acagctataa tgatatttac taaaacaggg acaggtggaa ctgataaggt atggttctat
    20821 gatatgaaag ctgatggtta ttctttggat gataagagaa atgagataaa ggatagtgat
    20881 atagaggata ttattaagag atttgataat cttgatgggg aagtagatag aaaaagaaca
    20941 gaacaaagct tttttgttga tgtggaagag ataagagaga atgggtatga tttatctatt
    21001 aataagtata aagaggtagt gtatgaggaa gtagtttatg atgctcctag tgtgattata
    21061 ggtagagtta gggagttaga gaaggaaata gctgttggat tagaagaatt agaaagaatg
    21121 ttagaggtat agggtatgga gtatataaaa ttaaatgaat tgtgctatat aaatattggc
    21181 aaaacaccat ctagaaatac ttctgattat tggggaagtg gtaatagatg gttatcaatt
    21241 tctgatttga aagaaaaata tatatcaaaa tcaaaagaag agataacaga tttagctgtt
    21301 gaaaaagcta atatgaaatt agtacctaaa aataccgttg taatgagttt taagttgtct
    21361 ataggtagag ttgccatatt aaaagaagac atgtttacaa atgaagcaat tgctaacttt
    21421 caaataaaaa ataatgaact tataacatct gaatatttat attatgcttt gagaacttta
    21481 aattttaata atactgatag agcagttatg ggtgcaactt taaataaaag caagttaaat
    21541 gatataaaaa taccatattt cacaatttgt atacaaaata aaatggtaga agttttaaat
    21601 aaagctcaag agttaataaa taaaagaaaa gagcaaatag aagcattgga tgaattagtt
    21661 aaatcaagat ttattgagat gtttggaaat gtgattacta atagtaaaga ttgggataca
    21721 gagttattag gagagatatc aaacttaaaa gctggaaaaa atataaaagc aaaagatatt
    21781 tatgaaaaaa attcacatga attatatcct tgttatggag gaaatggttt aaggggatat
    21841 gtaaaaatgt attcacataa gggaacattt aatctcatag gtaggcaagg agcattatgt
    21901 ggtaatgtaa aatatgtaaa tggaaagttt tatgctacag aacatgcagt tgtagtacaa
    21961 cctaaagttg atataaatag ttactggcta tattttacac ttaaagagtt agatttaaat
    22021 cgattatcaa ctggagcagc acaaccaggt cttacagtag gcaaattaaa tgaagttgaa
    22081 atacctaaag tacctgtata tcttcaaaat aagtttgtgg actttgtcag acaaatagac
    22141 aaattgaaat caagaatgga agatagctta aaagaactag aaaacaactt taactcatta
    22201 atgcaaaaag cttttaaagg agagttattc aactaaaata caagtattaa aatgggggaa
    22261 taaaatctat gaatcaaata gagctagatt tataccatga catggtaaat atctataaag
    22321 aagctgattt acaatgtaac tataagccaa caagattttt acaactgata aataacaaag
    22381 gagccttatt agctgctaaa gaattaatca ataaagaagg agctactgaa ggatttacaa
    22441 ggctatggga gtgtaagaga ttagatttat cattagaagt attagtatta aaagaaaagt
    22501 ataaagagtt gtttactgat gaagaaagag aaatatgtat aaatagatta aaagattatg
    22561 gatataaatt taattaagca atattaccta ataaacaaga ggtgattaaa tgagaaatat
    22621 accaaatgag attaaaaact caataataaa tgtatttcca aaagaaaata ctctagcaat
    22681 cataatattt ggtagttatg gaacagataa acatacatca gaaagtgata tagacatagc
    22741 ttggattccc atcaaaaaag taccaattac agaacttgca actaagacag aagctctaag
    22801 aaatatatta gacattgatg tagacctaaa aattgttaca gataattata caatagcact
    22861 taaaaaagct attttagaag gtgaagttat ttatgaatct aaagatttta aggattatat
    22921 aaataaattc tattttgaaa acagtgatgt aatagatatc ttagattgga gggacatgta
    22981 tggtaattag aaacaaatta tcttggaact caatagaaga agtaaatagg gattttggtt
    23041 cttgcttgta tgacctgcaa aacttcaaaa tcttatatga taaggaagaa atgcctgagt
    23101 tatgggctag atacataaaa gaaggcttta aaagctatat ggtatcattt ctagaattaa
    23161 caaaagcaat gttgtattat aaatcaattg atttaaacat aaaatctaaa aacttttatg
    23221 actacctttt agcttgtgag tatcacaatt tattacctaa aaattcttct atagttatag
    23281 aaacacttag aaaattgaga aatgatgatt ctcatggata tgatatacca cagtttgaag
    23341 atatgtacga attgtttaca gaaaatgaag aagtatttgt tagtataaga aacagctgta
    23401 agaaatagtt tttagagaga tttattttaa ctaagggaca aaagggggat aaaagcttgg
    23461 gtaattttga ttttttacaa aagaagaaaa atttcaatag ttttaatact gcttgcttag
    23521 aagcagaaaa aagtatacta gttagtccat ctacttgtgc aactataaca agaagagctt
    23581 tagagcttgc agtaaaatgg ctatatgcta atgatagtga tttagtattg ccttatcaag
    23641 ataatctcag tacgttgatt cataataata gttttataga acttatagac tatgatatgt
    23701 tacctttatt aaaatatata gttaaattag gtaatttatc tgtacatact agtgcgaaca
    23761 tagagagagg agaagcaata ctttcactta acaatttgca tcaatttgtt agttggatag
    23821 actattgtta ttcagatgaa tatacagcag aagactttaa tgaagattta ttactttatg
    23881 gggaagagaa aaggacgaga ccggacgaac ttaaaaatct ttatgagaga ttaagttcaa
    23941 aagataaaaa gcttgaagaa gtaataaaag aaaatgagga attaagaaag tcactaactg
    24001 aaaagagaaa agttaatact gtaaactatg atttcaagat agacgaagta agcgaatttg
    24061 agacaagaaa aagatatata aatatagaat taaagcttgc aggttgggaa tttggaaaag
    24121 atatagggga ggaaatagaa gtacaaggta tgcctaatga aagtggtgta ggatatgtag
    24181 attatgttct ttatggtgaa aatggtaagc cattagcagt agttgaggcg aaaagaacga
    24241 gtaaagatcc aaaaattggt caacaacaag ctaaattata tgcagattgt ttggaaaaga
    24301 gatatagtca aagaccagta atatttttga ctaatggttt tgatatgtat atttgggatg
    24361 actattcaga tagaaaagtt tatggatttt ataaaaaatc agaattacaa cttatgatag
    24421 atagaagaaa gagtaagaag tctttaagtt ctgtaacaat aaatgatgaa atatccaata
    24481 gatattatca aaaagaagct attagggctg tatgtgaggc tcttgaaaat aagcaacgca
    24541 aaactctttt ggtatgtgct actggtacag gaaaaacaag aattgctata tctatagttg
    24601 atgtactttc aagacataat tggattaaaa atatattatt tctagcagat agaaaagcat
    24661 tggtaaagca agctaaaaaa agttttacta agctattacc taacttggcg ttatgtaatt
    24721 tattggatag caaagacagc ccagaagatg ctaggatgat attttctaca tatccaacaa
    24781 tgatgaatgc aattgatgat acgaagtcta aggatggaaa gagattgttt acacctggcc
    24841 attttgattt aattataata gatgaatcgc atagaagtat ttataaaaaa tataagtcaa
    24901 tctttgatta ttttgattct tacttaatgg gtcttactgc tacaccaaag gatgagatag
    24961 ataaaaatac ttattcagtt tttgacatgg aaaatggagt acctacatat gcttatgagt
    25021 ataataaggc agtcgaggat ggatatttag tagattatac tagtattgag tttaagacta
    25081 agattatgga agatggaatt aagtatgatg agctttctga tgaagagaag gaagaatatg
    25141 aaaatacatt taatgatgat gagagtattg gagacgaaat agggaataat gcagtaaatg
    25201 agtggctatt taattctgac acaatagatt tagttttaaa taagctaatg acagaggggt
    25261 tgaaaataga aggagaagaa aagattggta agactataat ttttgcaaag aatacaaagc
    25321 atgctagggc aatagttgaa agatttaata agctgtatcc aaagtatggt gggaactttg
    25381 taaaagcagt tgactatagt ataaattatg tagattctat aatagatgat ttttctgata
    25441 agaataaatt acctcaaata gcagtttcag tagacatgct tgatacagga attgacatac
    25501 ctgaaatact aaatcttgtt tttttcaaga aagtgagaag taagactaag ttttggcaaa
    25561 tgataggtag aggaactaga ttatgtccag atttattagg tgttgatatg gataaggaga
    25621 gattcttaat atttgatttt tgtaataatt ttgaattttt taaatttaat cctaaaggat
    25681 ttgagggaaa taaagcagaa acacttactg aaaaattatt taatataaag gtaagtatgg
    25741 ttaaagaact tcaagatatt aaatatatag aaggtgagta tgcagaactt agaaaagaat
    25801 tgttagaaga acttataact tctgtaaaag ctttaaatga ggatagttat atagttagga
    25861 tgaatttaag ttatgtacat aagtataaaa atgaaaatgt ttggagcaat attggagcag
    25921 tagcacaaaa tgagataaga gaatatatat caccactcat aacttcatat agtgatgatg
    25981 aattggctaa aagatttgat gtagttatgt ataatataca acttgcatac ttacaaaata
    26041 ataatgcaag taaaggtata aggcatgtaa tggctacagc agaaaaactt tcaaaacttg
    26101 ggactatacc acagattcag caacaaaaat atactattga aaaagcaatg acagaggatt
    26161 tttgggaaga ttcagatata tttgaggtag aagaagtacg tatatcatta agagagctta
    26221 ttaagtatct tgaaaagtct agtcaaaaga tatattatac aagttttgaa gatatgattg
    26281 tagcagaaga tagaaatgat tctgtatata atgcaaacaa tcttaagaat tataaaaaga
    26341 aggtagagta ttatttaaat agccataaag atgaattagc aatatttaaa ttaagaaata
    26401 ataaaaagat aacaaagcaa gatgttgaaa cgttggagga aatactctta aaacagttag
    26461 gtaattgtga tgattataag aaggagtttg gagatactcc agtaagccaa ttagtgagaa
    26521 gactagtggg attggataga gaagctgcaa atgaagcttt ttcagagttt ttaaataata
    26581 agagctttaa tactaagcaa atacattttg ttaaacttat agtcgattat gtagtcaaga
    26641 atggatttat agaggataac aaagtgttga tggaagaccc atttagaact gttggaagta
    26701 taattgattt atttgaaaat catattgaag agagaaataa attgattaaa actataaata
    26761 aaattaaaga aaatgcatca gagataggct aatgatgtta tttagtgcaa aattattgtt
    26821 aatgatgtag agaataaatt tattagtagg tagtctaata ttgggttgaa atagataact
    26881 agatatggat aatgtaaata tacaaagaga ggtattgaat aattatgcaa tatctctttt
    26941 taattgataa ataactatga attactcatg aagaagatta aataataatt tttaaaatta
    27001 agtatttgta tattttatag aataatcatg tatttacttt aataaaataa aaacaggtat
    27061 aaaaaggtat ataataaggc tttcatatac atttatgagt caaaattttg atataatata
    27121 tagatacatg gagttatgaa tggaatttat ttaacttata gggggagtta atatgaaagc
    27181 aggagaaatg gaatttctta gttatttaga ggggtcaaac aaaagttttg tagtacctgt
    27241 atatcaaaga aattataact ggaagaaaga acaatgtaaa agattgtttg atgatttaga
    27301 ggatataata gattctggat ttagaactca tttcttaggg tcagttgtat ctatatatag
    27361 tatgggaaaa gaatatttaa taatagatgg acaacaaaga gtgactactg tgtcaatatt
    27421 attgattgcc atgtgtcaaa taataaaaga gtttggggaa tcaaaaggaa ttattacaac
    27481 agaagaacag attaaggagt gttatcttat aaacaagcac tcaagagata aagatggaaa
    27541 aataaagtta aaaccaatag cagaagatag aaaagcgtac tatgaactat ttgaagaaga
    27601 aatgaataca gatagaaatt ctaatatagt ttcaaattat ttatattttt ataatagaat
    27661 aaaagaagat aatatatcaa tagatgattt gtttgatgct atacagaaat taactatagt
    27721 agaaatagag ttaaaaaatg gtgaagatga ccctcaatta atctttgaaa gtctaaattc
    27781 aactggctta gatttaagtg aagctgatag agtaagaaac tttgtgttaa tgggtagaag
    27841 ctctaagtta caagaggaat tttataataa ttattggtat aaaattgaaa aaaatacaag
    27901 tagcaacgta agtagtttta taagagacta tttgactata aaggagagaa gtataccgaa
    27961 taaaaataaa gtatatttta gttttaaaag gtatgtatca gataatggta tagatataga
    28021 aattttatta aaagatttac ttaaattttc taactattat agagagatat cttattcatg
    28081 tactgatgat aatgatgtaa acaaagtttt gaaaaggata aacaaacttg aaatatttgt
    28141 atcatatccg tttatattag aattatttga tgattataat gaaggtataa taaataaaaa
    28201 agtacttgta gaatccctta atttagtaga atgttttata ttaagaagaa tagtatgtga
    28261 tgtacctccg aatgcactga ataaagtatt tatgaactta ggaagggaaa ttaaaaaatt
    28321 tagtgattat aaagaaaact attctaatat attaag
//
LOCUS       DAFKMX010000029        28322 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFKMX010000029 DAFKMX010000000
VERSION     DAFKMX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.029"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            123..686
                     /gene="raiA"
                     /locus_tag="LZB08_001056"
     CDS             123..686
                     /gene="raiA"
                     /locus_tag="LZB08_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBM7314569.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            899..1135
                     /locus_tag="LZB08_001057"
     CDS             899..1135
                     /locus_tag="LZB08_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBM7314570.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            1148..2491
                     /gene="yqfD"
                     /locus_tag="LZB08_001058"
     CDS             1148..2491
                     /gene="yqfD"
                     /locus_tag="LZB08_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBM7314571.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            2430..3545
                     /locus_tag="LZB08_001059"
     CDS             2430..3545
                     /locus_tag="LZB08_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBM7314572.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            3602..4063
                     /gene="ybeY"
                     /locus_tag="LZB08_001060"
     CDS             3602..4063
                     /gene="ybeY"
                     /locus_tag="LZB08_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBM7314573.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            4069..4788
                     /locus_tag="LZB08_001061"
     CDS             4069..4788
                     /locus_tag="LZB08_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBM7314574.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4907..5308
                     /locus_tag="LZB08_001062"
     CDS             4907..5308
                     /locus_tag="LZB08_001062"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBM7314575.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            5372..6265
                     /gene="era"
                     /locus_tag="LZB08_001063"
     CDS             5372..6265
                     /gene="era"
                     /locus_tag="LZB08_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBM7314576.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            6277..7656
                     /gene="mgtE"
                     /locus_tag="LZB08_001064"
     CDS             6277..7656
                     /gene="mgtE"
                     /locus_tag="LZB08_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBM7314577.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            7729..7884
                     /locus_tag="LZB08_001065"
     CDS             7729..7884
                     /locus_tag="LZB08_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBM7314578.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7850..8623
                     /gene="recO"
                     /locus_tag="LZB08_001066"
     CDS             7850..8623
                     /gene="recO"
                     /locus_tag="LZB08_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBM7314579.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            8616..9215
                     /locus_tag="LZB08_001067"
     CDS             8616..9215
                     /locus_tag="LZB08_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBM7314580.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            9375..10253
                     /gene="glyQ"
                     /locus_tag="LZB08_001068"
     CDS             9375..10253
                     /gene="glyQ"
                     /locus_tag="LZB08_001068"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBM7314581.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            10253..12319
                     /locus_tag="LZB08_001069"
     CDS             10253..12319
                     /locus_tag="LZB08_001069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBM7314582.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(12485..14032)
                     /locus_tag="LZB08_001070"
     CDS             complement(12485..14032)
                     /locus_tag="LZB08_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBM7314583.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            14312..15079
                     /locus_tag="LZB08_001071"
     CDS             14312..15079
                     /locus_tag="LZB08_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBM7314584.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            15362..15559
                     /locus_tag="LZB08_001072"
     CDS             15362..15559
                     /locus_tag="LZB08_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7314585.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            15588..16664
                     /gene="vorB"
                     /locus_tag="LZB08_001073"
     CDS             15588..16664
                     /gene="vorB"
                     /locus_tag="LZB08_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBM7314586.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            16667..17386
                     /locus_tag="LZB08_001074"
     CDS             16667..17386
                     /locus_tag="LZB08_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBM7314587.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            17399..17938
                     /locus_tag="LZB08_001075"
     CDS             17399..17938
                     /locus_tag="LZB08_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBM7314588.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            18095..19153
                     /gene="buk"
                     /locus_tag="LZB08_001076"
     CDS             18095..19153
                     /gene="buk"
                     /locus_tag="LZB08_001076"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBM7314589.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLLGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            19171..20064
                     /locus_tag="LZB08_001077"
     CDS             19171..20064
                     /locus_tag="LZB08_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBM7314590.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            20094..21302
                     /locus_tag="LZB08_001078"
     CDS             20094..21302
                     /locus_tag="LZB08_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7314591.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            21326..22585
                     /locus_tag="LZB08_001079"
     CDS             21326..22585
                     /locus_tag="LZB08_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7314592.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            23230..24693
                     /locus_tag="LZB08_001080"
     CDS             23230..24693
                     /locus_tag="LZB08_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBM7314593.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            24749..25885
                     /locus_tag="LZB08_001081"
     CDS             24749..25885
                     /locus_tag="LZB08_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7314594.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            26121..27236
                     /locus_tag="LZB08_001082"
     CDS             26121..27236
                     /locus_tag="LZB08_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBM7314595.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEILEGQINKSNVVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            27388..28257
                     /locus_tag="LZB08_001083"
     CDS             27388..28257
                     /locus_tag="LZB08_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7314596.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        11107 a         3033 c         5271 g         8911 t
ORIGIN      
        1 taaaaataat cattttcata tacaataata ttaatttaaa tacaataaat ttataaaaat
       61 tgtatttaaa gattaacata ttaaagtatt agaattggaa aggcttgaag ggagaggttt
      121 ttatgcaaat aatagtatct gggagacaaa tgaaattaac tgacggaata aaaggatacg
      181 tagatggaaa attaagtagg ttagaaaaat accttgatcc tgaatcagaa gtgaaagtaa
      241 cagtaagtgc gaaaaaggat agacaaaaag tggaggttac aataattcct attaacggtc
      301 aaataataag agcagaagat gtagaagatg acttatatgc agcaattgat attgtttgtg
      361 acaaattaag tagacaagtt gtcaaatata aaactaaagt taaagacaaa gttcaaaata
      421 ataagagcat aagatttgaa aatcttgact ttatagataa tagcagtgaa tttgatgatt
      481 atgattatga tgatgaagaa gatgaaaata tagtaataga aagaagaaag aaatttaatg
      541 taaaacctat gagttctgag gaggcgatac ttcaaatgga actagtagga cacaatttct
      601 atatgtttag aaaccaagat aattttgaaa taaacatagt gtataagcgt aaagctggtg
      661 gatatggttt aatagaacaa gattaataaa aattgtatac gtttagattt tatataagaa
      721 aaaataaaaa ggtaatcatg ttgatttttt atcaatatga ttaccttttt cttgtttaaa
      781 taatatatta gatattaggt atttattata taaaaatttt aaaactaatc tgttacaaca
      841 tcaataaagt gcataaatac ctatttcaag cataaaatta attaaggggg taatgcttat
      901 gttagaaata tccactgatt tacaagtaaa tcaaccagtt attactgtta caagtaatac
      961 gtttattagt atagaaaatt atctatcaat attagaatat gaagttgatt taataagaat
     1021 aaagactaaa gttaaaacaa ttaaaatatc tggggataaa ctttcactta agtatattac
     1081 tgatagtgag ataggtataa aagggattat ctacaatgtt gagtatgttg attaggagga
     1141 gatacacttg ataagtttta taaggggata ctatgttata gttgtggaag gagtaggact
     1201 cgaacaattt ttaaatcatc ttataagaaa tgggataagt gtttacaatg taactagaat
     1261 aaaaaatact aaaatggagt ttcatataga tagacaagat ataaaagact ttaagaatgt
     1321 ttatagagga agtaagtttg atataaaggt aaagcaaaaa actggggtcc cttttattat
     1381 caaaagagtt tataagcaca aaggtatgtg gatttgtgct cttgtttctt tatttttatt
     1441 gatgtctaca tcgcagtttg tgacagatgt atatatacaa tctcctgaag gtataaaaaa
     1501 agaagctctt agaaatgagc tttataaggt tggagtaagg ccaggagtct ataagaagag
     1561 tattgataga aaagaagtta gagaccatat gatgtcaaaa tttaatgatg tggcatattt
     1621 atctatcaat gtaaaaggaa caaatatatt tgttactgtt acaaaaaagg cagaatccct
     1681 aaaatctaca gaccaatcaa attattgtaa tgtaattgct ttaaaaaatg gtataataga
     1741 aaaggtaatc cctagaagtg gtaaatcagt tattaaatct ggagacatag tccaaaaggg
     1801 agatgtctta ttaaatggag ctaataccaa gtcaatacca gaagtatggg caagtacttt
     1861 ttatgagtcc acaaaaaagg caagttatgt agatactgta aataaaaaaa ctggagaaaa
     1921 gaaaaatata tatacactaa gtttttatga taaagagttt acaatgagaa aaaatataaa
     1981 atataaagat tatgttgttg aaaataaaga aaaaaaacta tcaataggaa actatacttt
     2041 tccaataaag ataaaaacga gtacttttta tgaaacaaaa aaggttgaag taaaaaggaa
     2101 taaagaagag ttaaaaaaag aattatctga aaaagcttta aaagagttag aatacataat
     2161 cccagcttcg gctagaataa tagatgtaaa acataacttt aaggttaata aaaatatgtt
     2221 agaatattta ataacagttc aaactagtga aaatattgct aaaatttatc ctcttagtaa
     2281 gtcagaagca gaaaggttta taaaggaaga aagtaaacca gatgaaggtg aagaggaagt
     2341 tccttccaat ccagaaaaaa gaccattaaa tgatataaga aatgagtttg atgaggataa
     2401 taaggataat aaagaccaaa acaattctga tgaaaataat agtaatcaaa atagtaataa
     2461 tagtcaaaat aataatagca ataacaacta ataaaattaa acttaaaatc ttaggaggac
     2521 taattcaatt gataatacaa aagaaattta caatagcaga tggtaacttt gaaagagagt
     2581 tatttggcaa ctttgatgaa aatgtaaaat taatagagaa gactttaaat atagatgtaa
     2641 tacttagaga aggcaatata attttaattg gagaagaaaa aaatgtagat tctgcattaa
     2701 aacttatgaa tgaattacac caaactgtat caaatggtaa gcatttagat aagcagagta
     2761 tttcttattc attatctctt ttgcttgaag gaagtgaaca aaaaataaaa gaacttgagg
     2821 ggacaatcgt cataactcaa aggggaaaag ctgttcaacc taaaacttta ggtcaaaaag
     2881 aatatataaa acttatagaa aataatgata taacttttgg tgttggacct gctggaacag
     2941 gaaagactta tcttgcggtt gctatggcag taaaagcttt taaaagagat gaagttagta
     3001 gaattatact tacaagacca gctgtggaag caggagagag tctaggtttt ttaccaggag
     3061 atttaaagga caaagttgac ccatatttaa gaccactata tgatgcctta tttgaaatgt
     3121 tgggtgctga taaatttaat aagtatttag aaagaggaac tattgaggtt gcaccacttg
     3181 catttatgag aggtagaaca cttgataatt catttataat attggatgaa gctcaaaata
     3241 ctacttctga acaaatgaaa atgtttttaa ctagacttgg atttggctct aaagctgtag
     3301 ttacaggaga tgtaactcaa acagatttac cacaaaataa gaagagtggt ctaatacaag
     3361 ctacagagat tcttaaaggt gttccaggta ttgggtctat aatgctaaca gatagagatg
     3421 ttgtaagaca tgagttggta cagagaataa taagagctta tgagaagcac gacaaaagag
     3481 aagaatttaa aaaagaagaa cgtaaaaaag cgaaaatgca ggaaaagact ttcaaaagaa
     3541 agtaaaagtg tagtaatata gatatatagc attattaatg aataaaaagt aggtgtagtg
     3601 aatggatttg atactagatg atagacaaga taaattagaa gtaagtgagg agttaattga
     3661 aaagataaag gatataatca tagaatgttt agattatgaa ggatatgatg ataattatga
     3721 agtgagtctt tcttttgtag ataataaaga gattcatgaa ttaaatagag aatatagagg
     3781 agttgataga gtgactgatg ttttatcatt tcctctttta agtgatgatt ttgaggatgt
     3841 tgagttagaa gaagaatccc taggtgacat agtagtatct cttgaaagag ccttagaaca
     3901 gagtatagag tataaccata gttttgaaag agaagtatgt tttttaatat gtcatagtat
     3961 gtttcatctt ttaggttatg accacgatac agatgaaaat acaaaagaaa tgagagaaaa
     4021 agaagaacac atattaaata agttaaacat aacaagggag tagtttttat gaaaccagaa
     4081 aaaactcgtc aaggtataat taaagcattt aatgcagcta tagaagggat attgtataca
     4141 tttaaatttg aaagaaatat gaaaatacat tatttaggtt cagttgctgt tcttataata
     4201 agtttgtttt ttaacttttc aaaattagaa atgataatgt tacttatgtc catatgttta
     4261 gttgtagttg ctgaaatgtt taatacagct atagaaaaag ctgttgattt agtaactgat
     4321 gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag ctggaggcgt tctagtggca
     4381 gcactaaatt cagtagtagt tggatatatt ttgttttatg ataaattgac agatatatct
     4441 ggaatattaa tttataaaat aagagaatca gaattacata taaccttgat atgtatatta
     4501 ttggttctta tagctgttgt agttgtaaaa gcattgactt ctacaggtac tcctctaaaa
     4561 ggaggaatgc caagtggtca tgctgcctta gcatttgcta tagcaactgc aattacactt
     4621 atgactgaaa gagttgttgc ctctactctt gcatatataa tggctgtatt agttgcccaa
     4681 agtagaattg aaggaaaaat acataccttt tgggagacta ttgctggagc tttattaggt
     4741 gtgttgatag ctatattagt atttcagctt ggaatgttct ataattaaaa taaatgtaca
     4801 taaaatattt aaaataaaac aaatctgtgc taattaaatt tttatgatat aatgtagagt
     4861 attaaataaa aataactttt aattattgta tacaggagga gttgttgtgg ataacataga
     4921 attgttaagg ttggcagaag atgccagaca acattcttat gctccttact caggttttag
     4981 agtaggtgca gctcttttaa ctaagagtgg taaagtttat actggatgta atatagaatg
     5041 tgcatcatta ggtggaacaa attgtgcaga aagaacagct atttttaaag caatatcaga
     5101 gggtgataag gatatctata aaatagctat agcaagtgat aactcagaaa ataatgagca
     5161 aacatatcca tgtggaatat gcaggcaggt aattatagag tttggttctg atataaaaat
     5221 aataacggga tatactaaag gagaggtgtt tgagcatagt ataaaggatt tgttgccaaa
     5281 ctatttctct ggaaatgatt taaaataaat ttaaaagtat aaataaattt aagtaattaa
     5341 aacaagaaga taaaaagtta ggagacagtt aatgttcaaa tcaggttttg tcagtatagt
     5401 gggtagacca aatgtaggaa aatctacttt aatgaataat gtagttggag aaaaaatagc
     5461 tataatgagt gataaacctc aaactacaag aaatacaata caagcagtat atacagatga
     5521 agaaatgcag atagtattct tagatacacc aggtattcac aaaccaaaaa ataaattagg
     5581 tgaatttatg gtaaaggcag caacagaagc ttttaaaaat gtagatttaa tattatttgt
     5641 agttgatgat tctaaaaaaa taggtcctgg agatagaaag ataatagaag atttaagaag
     5701 tgttaagaca ccaataattt tagttgtaaa caaaatagac caactagacc aaaaagatga
     5761 gctatttgat ataatcaaaa tgtatgatag agaaggtatt tttaaagaaa tagtaccaat
     5821 atcagcatta aaaggaaaga atacggatac cttaataaaa gttatacaaa attatttaga
     5881 agaagggcca aagtattttc cagattatat gattacagac cagccagaaa gagtattaat
     5941 agcagaactt ataagagaaa aagttcttca ctatcttaat gatgaaatac ctcatggtgt
     6001 agctgttgaa attgaaaaaa tgaaagcgag aaatgataag gaaatagtag atgtatctgc
     6061 tgtaatatac tgtgaaagag attcacataa gggaataata ataggaaaaa atggtaggaa
     6121 actaaaagga ataggtaagt cagcaagaca agatatagaa ttgcttttag gttcacaaat
     6181 aaatcttcaa ttatgggtta aagtcaaaga aaattggaga aatttacaaa attatataaa
     6241 taattttgga tataatgata aataaaggtg ggttatatgg ataggaaact ggatgcttcc
     6301 caagatttat tatatgaagt aaaatcatta attgataata ataaagtatt ggagttaaga
     6361 gaattaatag aggaatatca tataattgat atattcgata taatggaaaa tttagaggaa
     6421 gacatgaaaa ttcaattatt tgaagtactt cctttagata tggcatcttc tatattagaa
     6481 gaaggtagtg tagagttttt tattagtatt ttatctaaac tggatgtaga acactcgaaa
     6541 aatatacttg aacttatgtc tcttggagat atggcagaca agttgagtga acttgaagaa
     6601 gaagaaagag aacacataat aaatcttttg aatcaagaga atgccgatta tgtaaaagag
     6661 ttactattct atgatgaaga ttctgctggt ggaacaatga ccactggata tatttctata
     6721 aataaagata tgacagctct tgaggctata gaccatatga gagaagaagc tgaagaagct
     6781 gaaactattt attatatata tgtggttgat gatgaagaaa aactagttgg agtattgtca
     6841 ttaagagaat taataatagc tagagacgca aatatagtag aagatttgat gagtgaaaat
     6901 ataatctctg tttatgtaga tgaagataga gaagaagcag taagattagt atctaaatac
     6961 aatttaatag caattccagt agtagacaga caagaaaaat taaaaggaat aataacagta
     7021 gatgatataa ttgatgtaat ggaagaagaa gcaactgaag atatgtataa atttgcagga
     7081 agttcagaac atgaaagaga agttgctgaa aaagaaaacc ctacattgag agagcaaata
     7141 atatcagcac ttagaggacg attaccttgg ttaattatta ccttagttgg agggttgttg
     7201 gcttcgttaa tactttctaa cttagactat ataatgaatc ctgtatatgc tccattagta
     7261 ttttttatac ctgtagtaat tggtatgggt ggaaatattg gaactcaatc atcatctgtt
     7321 acagtaataa cattatctaa taaggattta aattttagca atgtggttag agaaggtatt
     7381 gtaggtatta ttacaggtct tttgtgtagt ataataacag gtattgtaat ttactttgtt
     7441 atgagaaagt tagacattgt attaattgtt tcaatatcat tatttataaa tatggtttta
     7501 ggtgcaacaa taggtgcgtt tatgcctgtt ttattaaaaa aaatggatgc agacccatct
     7561 acagtttcat caccaattat atctacagct cttgatataa ctggtatagc agtttacttt
     7621 ataataacaa cggcattatt gtcaaaaatt gtttaataac tttatgtatg atttatcctc
     7681 ttcttactaa aaatataagt ataaatgtaa gaagggggtt ttttaaaaat gaacaatatc
     7741 agttgggaag tttttaaaaa aacaggtagt atagatgcat atttatattt ctgtgattgc
     7801 aaaaacatac atgaagaaat tgaagaagta agggagaaaa aagaagacga tgataatcct
     7861 gaacacccag gggatagtac ttaaagctat aaggtataag gaaagtgaca taatacttac
     7921 actatttaca agaaagcttg gaaaagtatc tgcaatagca aaaggagcta aaaaaaataa
     7981 gagctctttg ctttcatcat cacagttatt ttcttatagc aattttacct taaaaaaaca
     8041 aggtaatatg tacaaggtta ctcaaagtga aataataaaa agtttttata atatatccta
     8101 tgatatagag gcattttctt atgctacata tattacaaaa ttagttgaaa attctatact
     8161 tgaaaatcag actaataata ggctatttat attacttgcg cagactctgt atttgtatac
     8221 acaggataat acagacaata ggtttataac agcagcattc gagttaaaat ttttagacta
     8281 tataggattt aaacctatag taaataaatg cattaattgt gaatgtaaaa ttttacaaaa
     8341 ttctgtgttt aatatctacg aaggtggtat actatgtagt aagtgtagta aactttttga
     8401 taataatata aaattagact taacaactat aagtcttatg gaatatgtat taagaaatga
     8461 tattttaaca tgtagtaaag ctaaagtatc aaaatatata acacatgaac ttgagaatat
     8521 tctagacaag tatttgaaag tttatgttga taatattaat tttaaatcat tacacgtatt
     8581 gcaaagtgta aaaaataata agggagtgga taatgatgag tagtaatata actattgaaa
     8641 tggttgattc agtctttgaa agagttccag gagcaactta tgcagaagca aaagaagcat
     8701 tagtaatgtg caatggagat gtaattgagg cagtaatata tttagaaaat aaaaaaaata
     8761 cttgtaattg taaagcaaaa tctgctaaag aaactatgga agaagtattt ggcaaagatg
     8821 gagaaaatat aaagtttcaa ttaaaagagc ttcttagaaa aagtagtgta gtgagaataa
     8881 tagttgaaaa agatggaaaa actatgatga acattccttt aacagttgga gttgttggat
     8941 tagcttttgt accattagct actttagttg gattatcagc tgctgttata agtaaatatc
     9001 gtataaaagt tcaaaatgaa gaagatggag aaattgtaga tttaggtgaa ttgaatgaag
     9061 aaaaacttca cgttttgaag aatatgataa ctaatgcagc aaaagatgtg aaggatgtag
     9121 tagttgacaa aaaagaagat gacaaagatg taactgctga ccttatgaaa gaagaatctg
     9181 aaaagaataa agaagacgaa gacgaaaata aatagatata aagtgacttt taaaaaatga
     9241 agagaagata attataagaa ttatcactaa cattgacaaa ttttatagaa tttgatattc
     9301 taaaagttgt agaagaacta ttaaaatagc ctttcgtgta gacgagaggc tttattgtta
     9361 taggagggat ttttatgaat tttcaagaga tgatactggc gttgcaaaaa tactggtcaa
     9421 agcaaggttg tatcatgatg caaccttatg atattgaaaa aggtgcagga actatgaatc
     9481 ctaatacttt tttaagatca ttaggacctg agccttggca agtatgctat gtagagcctt
     9541 caagaagacc agcagatgga agatatggtg aaaacccaaa cagactatac caacatcatc
     9601 aattccaagt tatactaaaa ccttctccag ataatataca agaattatat ttggaaagtt
     9661 taaaagagat tggaattgac ccaagtgagc atgacataag atttgttgaa gataactggg
     9721 aagctgctac agttggtgct tggggacttg gatgggaagt ttggctagat ggtatggaaa
     9781 taactcaatt tacgtatttt caacaagtag gtaatattga atgtgagctt gagacaggag
     9841 aaattacata tggattagaa agattagcta tgtacattca agaagttgat agtgtttatg
     9901 atttaaaatg gaatgataaa ataacttatg gtgaagtatt taataaagct gagtatgaaa
     9961 actcaatgta cgcatttgaa ttatgtgatg cagatatgtt gtttaatctg tttgatatat
    10021 atgaaaaaga agcattaaga cttatggaaa atggacttgt aataccttca tatgactatg
    10081 tgttaaaatg ctctcatgca tttaatacat tggatgcaag aggtgcaata ggagttagtc
    10141 aaagagcttc atttataggt agagttagaa atatggcaaa gactgttgca gaaacttttg
    10201 taaaacaaag agaagaaatg ggattccctc ttttaaagga cggtgacaaa taatgaaaaa
    10261 ttatctttta tttgaaatcg gtgttgaaga aatgccatca agatttgtag gcagtacact
    10321 agaacaatta aaaaacaatc ttagtaaatt atttgatgaa aatagaatag cttttgataa
    10381 aataaatgcg tatgggacac caagaagact tactttagta gtcgaaggat tgagtgatag
    10441 acaaaatgat ttagaagagg aaataaaggg tcctgctaag aaaattgctg ttgatgcaga
    10501 aaataattta acaaaaccag cattaggatt tataaaaagc aaaggattaa gcgaatctga
    10561 catatatttt aaacaagttg gaaaagaaga atatatattt gcgactataa agcaagatgg
    10621 aaaagaaact tcagaagtac taaaagacat attaccagag actataaaat ctgtagtatt
    10681 cccaaaagct atgcgttggg gaggaaaaaa tcttaaattt gcaagaccta taagatggat
    10741 agttgcattg ttaaatgata aagtattaga gtttgattta gaaggaataa tatcttctaa
    10801 tgtaacaaaa ggtcatagat ttttaggtga aagtacaata gaagttgatt ctatagaaga
    10861 ttattttgaa aaattagaaa aaaattatat agttttagac caaaacaaga gaaaagaaat
    10921 gataagaaaa caatgtatag aagtagcaaa ttctttaggt ggagaagttg aatttgatga
    10981 agatttactt gaagaggtaa cttatttagt tgaatatcca actgcttttt atggagaatt
    11041 tgatgtggat tatataaaac taccaaaaga agttgttata actcctatga aacaacatca
    11101 aagatatttt ccagtactta aagaaggaaa actgttacct aactttatag ctgttagaaa
    11161 tggtgattct tacagaatag acaatgttaa agctggaaat gaaaaagttt tagaagcaag
    11221 acttgcagat gctttatttt tctatagaga agatactaag agaaacttag aaagctacat
    11281 agaaaaatta aaaactgttg tattccaagt aaaactagga actatttacg acaaaacttt
    11341 aagacttgaa aaattaagtg ctgatatact tgatagttta ggtttagtag atgaaaaggc
    11401 agatactata agggctgcta aattatctaa ggcagattta gttactggaa tggtaggaga
    11461 atttgatgaa ttacaaggtt ttatgggaaa agaatacgca aaagttggag gagagaatga
    11521 tgttgtaagt gaagctattt ttgaacatta tcttccaaga tttgcaggag atatacttcc
    11581 taagactaat gcaggagttg ccttatcaat agctgataag ttggattcaa tagcaggttt
    11641 ctttgctata ggaatacaac caacaggttc tcaagaccca tatgctttaa gaagacaagc
    11701 tttaggtata cttagtatac ttatggacag aaaagttgct gtggatataa aagtacttat
    11761 tgaacatgct ttagacaatt attctgaatt agattttgat aaagaagaag ttgttgagca
    11821 aataatgaat ttcttcaatg agagagtaaa aaatctattt aaagatttag gaataagata
    11881 tgatgttgtt gatgctgttt taagttctga tataaatgat gtttctgata tgcatatgag
    11941 agctttagag cttaataatt ggctagaaaa agatgaatta gtggagatgc ttactgcatt
    12001 taatagagtt tctactctag ctcaaaaagc agaaagtgat aaaattgatg aatctttatt
    12061 aaaagaagat gctgaaatta aactttacaa tgaatttaaa caagtaaaga taaaagttaa
    12121 tgaattatta aaggataaga aatatggtat tgctttagat gaatttgcat ctttaagacc
    12181 agtagttgat aatttatttg atacagttat ggttatggat aatgatgaag ctattaagaa
    12241 caatagactt gctttactta aacaagtata tgatactatg ctagaaattt gtgatttatc
    12301 taaaattgtt tataaataaa atttaaacta tttataagaa ataaggctgt ctcaaaatag
    12361 agatagtctt atttttcatt gttgagatga tgaaaatata tttgatttca tagaaaaaga
    12421 gtgtctcata aactaaaaag ttcattttga gacacccatg ttttttgtaa gtaaatataa
    12481 taaactatat tttatactta tcagttattt tgtttaaaat attatcattt gaatttaccc
    12541 attcataaaa attgcctgat gatgcgtccg atattttata aagcatttca ggaatatcac
    12601 tagctttaaa atctccaagt gattttccca atctagtctt acctatttca aaactgcctc
    12661 caacaaataa ttcaaatgca tctgtagata taccatcaac attcttcttt ttaccacata
    12721 aacctatgct accaacttga tgaacaccac atgaatttaa acatcctgat atatataact
    12781 taggtgcttt atttaaaatt tcttcattat tttgtaatct aaaataatct atgatttcat
    12841 gtaacatttt ctgactattt tgtatgccca tttgacaaat tggaacacct atgcaagata
    12901 tactttgttc taattgagaa ttacaactaa tagttttact tatttctaat attctttttg
    12961 cttcatttcc atttaaattt aaaatatata atccctctgt cattcctagt ctaatcatag
    13021 gattttcaac attatctaat tctttaagta aagtacttaa atcctttgtt aacaaaatac
    13081 ctccaactgg atgtatatat acagtgtata aaccatcctg tttttgaggt attagtctag
    13141 aatcttgtat atctatttta actccaggtt tagagtaatc tatttcttca atattgagtt
    13201 caagactacc attttctttt tctaatttat agtattcttt aaatttttct aagaatagtt
    13261 cttcaccaag tgattcgacc atatatctaa ccctagctct gtttttattt tcataatttc
    13321 cataatccat aaagaattta atcatacctt caacacaata taaggcatct ttcggcttta
    13381 taagttcatc aagttctaaa gcaactttgg gttgtttacc tagacctcca cctaagaaaa
    13441 ctctgaaata tggttcatca tttttcaaag tagcaacaaa tcctaagtct tgtatactac
    13501 aatgagcagt atctgtgtaa caattagaat aagaaacttt taattttcta ggtaaatggt
    13561 atgtagtagc atttttgata aaatatttat cagtagcaac tgcatatgga gtaacatcaa
    13621 agacttcact agggtcaact ccagataatg gagataatcc gacatttcta ggataattac
    13681 caccaccacc tctagtgaat atatcatttt ttattccttc ttccatgata tcaacaattg
    13741 aatttataga taaatcgtgt aattgtatag cttgtctagt tgttaagtgt atcttatcta
    13801 agttgtgttt aatagctatt tcatagattt tattaagttg atactgagat aaaactccag
    13861 cagatactct aagtcttatc ataaaagatt ttttgtctct ttgagcataa acaccatatc
    13921 caccagaaaa tcccttatat tgcattttat ttatttctcc atttaaaaat tttgaagaat
    13981 attctttaaa attaggtagt tcatttaaca atatcttttt aaaattatcc attatttcac
    14041 ctcattgtta gtcttgtata aaaatatatc tattgtataa tagatatata tattattatc
    14101 tagtcaggat aaaaatataa cagaataatt ctattaaaag taagaataat aaggacatag
    14161 tgagtatttt gtttttattt tacaaattaa gtataatatt tttgttttat aaaatataat
    14221 taagataaat agataatttt gtaattttaa tatataatat ttagtagagt gtgggagtag
    14281 cacttaatat ttgcaggagg agtttttgta tatgggagaa ataataaatg cattagatag
    14341 agccctagac ataattttgc tgttatatca tgaaaagaga gaaatgggaa taactgaaat
    14401 aagcaaggct atgggagtat ataaaagtac tgtacataga actttggtta ctcttgaaaa
    14461 caaaggtttt gttatacaaa atgcagagaa tggtaagtat tggctaggaa taaatttata
    14521 tgcaataggt atggttgtgg gagaaaaaat gtcattgact gaaatagtaa aaccatatac
    14581 gaaaaaatta aatcaagaat ttaacgaagt agttaatgta tctatattag aagaaagagc
    14641 tcaagattct ccaagaagta tcataataca taaagagtat ggttcaaatc aattactttc
    14701 agtaaatcca tccgttggtt caagtagtga atgttattgt tcagcagttg gtaaatgtct
    14761 tatggcattt aatgattcta tagattttga aaaatataga aaaacaccaa tacacaagta
    14821 tacagaacat acaattgata attgggatga tatgatgctt tttttggcaa aaataaaaga
    14881 acaaggatat gctatagatg atgaagaact agaacatgga ctaacttgta ttggagcacc
    14941 tatcttagat aaaaataata aagctattgc agccataagt ttgtcaggtc caactataag
    15001 aatgagagaa ggggactttg agtataagat taaaagagtt atagaaactg caaaaagtat
    15061 atctgaactt tttagatgat ttttaatctc gataataaaa ataggctata tttaaattgt
    15121 tatttgtaaa tatcagttta aatgtagcct atttttcttt aatctaaaat taaatattct
    15181 taaaatttaa aatgttattg aaatatataa gcgaatgtgt tatcttttaa ttaagcaata
    15241 aaaatagaac aaggttctat actatagaac aaaattgatg catatatagt atttatacaa
    15301 aatataaata ttatatattt aaaattaagt aaaaatatta cataaagggg agatttgaga
    15361 aatggaaaaa gtcaagttga gtgttgaaag atgtaaggga tgctatcttt gtattgaagc
    15421 ttgtaaaaaa gatgcaataa gtctttctga ctacacaaat aaaaaaggct ttgtaactat
    15481 taaagtcgac gaagaaaaat gtattcaatg tggcgcttgt tatacaatgt gccctgattt
    15541 agtatttgag atattataaa ttatttttat tagaggggag aaaaggtatg caaagtttat
    15601 taattaaagg aaatgaagct ttagcagaag gtgctatacg tggtggctgc agattttatg
    15661 caggttatcc aataacacca caatcagaaa tattagaaat gatggcatgg agacaaaaag
    15721 aagttggagg agtatttatc caaggtgaat cagaaatagc cagtgtaaat atggttatgg
    15781 gagcaagtgc acttggagca agagctatga cttcttcatc tggtccagga ttttcattaa
    15841 aacaagaagg tattgcatat tgggtatcag ctggtttgcc agcagttgta gcatgtgtgc
    15901 aaagatatgg tataggtgat ggatttatag gagctggtca agataattat tggcaagcag
    15961 taaaaggcgg tggaaatgga gattatcatt taatcgtgtt agcacctaat agtgttcaag
    16021 aaaatatgga tatggcatat gaatcttttg aattggctga aaaatatatg catccagtac
    16081 ttatactttc tgatgctaca ataggtcaaa tggtagaacc ttgtatagca ccaccaatga
    16141 aagaatacga tatggataaa gctccatggg ctgcaaaagg aacacctgta ggagaaaaaa
    16201 ataaagtaat aactgatata acatattttg aagaccaaga tacatggcaa aaagggtata
    16261 tagaaaaaat gagaaaagtt caagaagatg aacaaagatg ggaagagata gaaatagaag
    16321 atgctgaagt ggtatttgtg gcatacggta tttcatcaag aatagcagaa ggtgcagtta
    16381 gagatgctag agcaaaagga atgaaattag gtctaataag accaaaaaca ttgtggcctt
    16441 acccaagaaa agcatttaag aaattaagtg attcattgaa gggacttgta acagttgaaa
    16501 tgaacatgat ggcacagatg aaggaagatg taattatagc atgtgataat agatttccag
    16561 tatattcagt agcaacagct caatatatgg cagaagtaaa tcaattaata gattttgctc
    16621 aacagattat agatggaaaa gctaagcaag aggaggtatt ttaattatgg ctaaaaaagc
    16681 accagcaata atgatgtgtg aaaatatgtt ctgtggaggc tgtggacact ctatagtcga
    16741 tagaattatt ggagaagtat taacagaaat ggatatatgt caagatacaa ttatatgttc
    16801 agatataggt tgtaatcata tgtttcaatt tagtatgaat gtagatgttg tcgttccacc
    16861 tcatggtaaa atgggagcag caatggcagc tatgaaaaga gttagaccag ataaatatat
    16921 atttacaata gctggagatg gtggagctta tgcaataggg cttggagaaa ctatgtcagc
    16981 tgctactaga aatgataatg taatatttct tgttatgaac aataatatgt ttgcaatgac
    17041 aggagggcaa atggctccaa ctactataga aggtcaatat acagcttcta ctccagcggg
    17101 tagaaattac gcagatcatg gttcaacatt tgatgttgta aaagttatgg gaaatttaga
    17161 tatagcatat ctagcaagag gaacaataac taaccctaaa gaaataaata caacaaaaag
    17221 atacatcaaa aaagctctag aaaaacaaaa agaaaagaaa ggtttttgct taatagaagt
    17281 gcttgtacca tgtccaacaa actggggatt aaagccagta gatgctatga agagaattga
    17341 aaatgaactt gttccaaagt atccacttgg agaatttatt gattagggag gaaataatat
    17401 gttgaatgaa attatatgtg ctggctttgg aggacaggga gttcttacag caggaaaaat
    17461 tataatgtat gttgcatata aggcagaaaa gaaaattaca tggttcccat cttatggaaa
    17521 tgagatgagg ggaggaagtg caaattgcaa tgttgttgta agtgatgaaa aaattgcaaa
    17581 tccttgtgta aaaaatccag atatactatt gggaatgaac agcgtatcag tagataagta
    17641 tgaaagttta atgaaaaaaa atggttatat gtttgtcaat gaatcactag taagtaagga
    17701 tagaaagtac agagaagaca ttaatgttat aaaagtacct gttactgaaa tagcacaaaa
    17761 tttaggcaat gaaaaagctg ctaacatttg catgttaggt gctatggtaa agagtttgga
    17821 tttatttaca aaagaagaat ttgaaagtgg tatgtgtgaa tactttgaaa atcaaggcaa
    17881 aggtaaattc aattctaaaa atgtagaagc atttgaagct ggatacgact acgaatgaat
    17941 attttaaata tttaaaataa ttattgaaat acttattttt atatgttagt atttaattaa
    18001 taaaatatag aacgtcgttc tggacaatgg gacgaagtct ttaggttgga atacaagaac
    18061 gacgttctgt atagtagaac tagggggtgt taaattgaaa gttttagtaa taaatcctgg
    18121 tggtacatcc acaaaaattt ctgttttcca agatgaaaat gaaattttga aaaaaaatat
    18181 aacccataca agagaagatt taaaaaattt ttctaaagta tttgatgaat atgattatag
    18241 aaaacaactg atagtagata ttttaagtag tgagaatcat agtataaatt cttttgacgc
    18301 agtagttggt cgtggtgggc ttatgaaggc tataaaaggt ggtacctata ctgtgagtga
    18361 agaaatgatt gaggatatga gaaatgaaat aaatggggaa catgcatcaa atttaggtgc
    18421 attattagct aaaactatag ctgatgaaat aggagtacaa tcatttgtag tagacccagt
    18481 ttctgtagat gaatttgatg atgtatcgag gattacagga atatcagaca tagaaaaagc
    18541 aagttggtta catgcattaa accataaagc tgtttcaaga aaaattgcac aaaaactagg
    18601 gggaaaatac gaggattata actttattgt agctcattta ggttcaggaa tatcgattgt
    18661 agcacataaa caaggcaaaa tgattgatgg aagtggtggt aggtctgatg gtccattttc
    18721 tccagaaagg agtggtggat tacttgcata tcctcttata gaattgtgtt actccggaaa
    18781 atatactcat gatgaaatgg tcgcaaaagt aagttcgatt gggggaatgt acgattattt
    18841 aggaacaaag gacatgatag aaattgaaaa cagaatcaag gagggtgatg aaaaggcaaa
    18901 acttataatg gatgcattta tttatcaagt agcaaaagaa atttctatgt atggagcttc
    18961 acttttaggg aatgtagata gagttatctt gacaggaggg atatctcact cagaaatggt
    19021 agtcaaagga gtagcagaaa aagtttcata tctagctcca atagagatag taccaggaga
    19081 gatggaaatg gaggcacttg ctctaggggc acttagagta cttaatggag aagaaatagc
    19141 aaaaacttat taatatttag gagaaagagt atgttaaaag attttaataa tttattagaa
    19201 gaggctaaaa agcttgaacc tgttattgta agtgttgctg ttgcagaaga caaagaagta
    19261 ttaaaggctg tgaaatcagc aacagattta aacattataa agccaattct tgtaggagac
    19321 aaggataaaa tagaaagtat atcaagagaa attggattaa aaggtcatga aataataggt
    19381 tgtcaaaatc ccgatgaatc agttaaaaaa gctgtagaga tagttaaaaa taaggaagct
    19441 aaagttctta tgaagggatt agtaaataca agtgtctata tgagagctat attaaataaa
    19501 gaaacgggtc ttagaacagg aagattatta agtttgcttg cagtatatga attacctcaa
    19561 tatcataaac ttctttattg tactgatagt ggaataaatg tatcgccaaa cttaaatcaa
    19621 aaaaaagata taatgacaaa tgcacttctt actatgaaaa gtataggtat ggaaaatcct
    19681 aaaactgcga tacttactgc aaatgaaatg gtagacccta agatacaatc tacagtggat
    19741 gccaacctac ttgtagagat ggaaaaatcg ggagaaatac caaagtgtat agtggaaggt
    19801 ccaatagcat ttgatgtagc atttgatgca catgcagcag aacataaagg tattaaaagt
    19861 aatattgctg gtgatgttga tttattagta tttccaaata tagaagcagg gaatatatta
    19921 ggaaagtcat ggcttagatt taataaagct aagtgggcag gtatagtttt aggagcaaca
    19981 aatccgataa ttttaggttc acgttctgac acagctgaaa taaaaataaa ttcaatagca
    20041 ttagcgtgcc tagcatcaca ataagaaatt attattaagg aggaaaaagt attatgaaat
    20101 atgcaaaaag aatggatatg gtaaaagctt cagctataag agattctcaa aagaaaatag
    20161 ctcagaaggt agcaagtggt ggaactgtta tatcttttgc tgcagggctt cctgacccaa
    20221 atttatttcc aatagaagag ataagtgaaa taactcaaaa agtacttaat gaaaggggaa
    20281 aatttgccct agcatatgga cctactaaag gtgaagatga gttgttagat ctattagtaa
    20341 aaagaatgaa agaagaagaa aatattgata caaaagcaga aaatattata ataactactg
    20401 ggtcacaaca agggatagca ttaagtgcta tgattttatt agaaaaggga gatgtagtag
    20461 ttacagaaaa tccaagttac ttaggtgcaa taaatgcatt tagaccttat gaatgtgatt
    20521 ttttaggagt agatacagat gaagaaggaa tggtaacaga tgatttagac aaattattat
    20581 ctgaaaattc taacataaag gtgatatatg taataccaac atttcaaaac cctacaggaa
    20641 aaacatggtc tttacaaaga agaaaagatt ttatggaagt cgtgaataaa tatgatgtca
    20701 tagtaataga ggataatcct tatggagaaa taagatttac accagaacct ctacctacac
    20761 ttaaatctct tgatactaaa gataaagtat tgtaccttgg gtcattttca aaggtattat
    20821 gtccaggatt tagagttgct tggatgtgtg gtaatgtaga catcatagat aaggcagaac
    20881 ttttaaaaca aggtatagat ttacagtcaa atcaattttc gcaagtacaa gtaatagaat
    20941 ttttaaagaa atacaactta aatgaacata tagaaaaaat aagagcagag tataagaaaa
    21001 gatgtctgct aatgttagat gctatgaaga aacatttccc agaggaagct aaatatacag
    21061 aaccagatgg aggaatgttt atatgggttg aacttcctga aagtataaat gtagatgatt
    21121 tattagataa agccattgat gctggagttg cttatgtttc tggagagtcc ttctttgcaa
    21181 ataatggtcc aaaaaatact atgagactta actatacaac aatgtcagaa gaacaaatcg
    21241 ttaaaggtgt tgagatactt gcagaagtta taaaaaaaga actcaattat gcttgtgaat
    21301 aaatataaaa agtgagggtt taaatatggg agaaaaagaa aacggtataa agtttggaaa
    21361 gttaatgttt caacttgtta ttatagcaat atcttgggaa ttagtttata ttataccatt
    21421 tatccaatat acattatatg atccaatcct aaaagcatta gaatgtacaa acactcaact
    21481 aggttttcta cttacaattt atgggttagg taatatattt ggagctccta taggaggctg
    21541 gttagcagat agatttgatt atagaaagat aattttaggt tctgtatttc ttaatggagt
    21601 agtatcattt ttatttgcat ttaatatgaa ctatccattt gcagttgcta cttgggtagg
    21661 ttgtgcaatc acatcattag ttatgaacta tccttctatg gtaaaaatac ttagagttat
    21721 aggaaaagat aatcaaggta aggtttatgg atttaatgaa gctatggttg gagtttctgg
    21781 agtagtaatg ggagcaatat tcttatatat ttatacatgc tttgcaactc ctacattggg
    21841 tatgagatgg gtaatgatat ctttaggaat attatcaata gcaatgtgtc cagtattatg
    21901 gtttgtaatt aaagatgtag atattaaaga agaaagagaa gaagcaggtg aaaaaatgtc
    21961 tgctggtgat tttataacag tactaaaatc accaaatact tggctagtag gaataagtat
    22021 attctgtgta tattcattca cagttacaat gtcttatttt acaccatata taacatctgt
    22081 acttggaggt tctgtagcat tatctggtgc attagcaata ataagacagc atggtctaaa
    22141 attatttgga gcaccatttg gaggatattg tgcagacaaa ttaaaatctc ctacaaaagt
    22201 acttttacca atatatgtat ttggtatagc agtaatagtt ttattcctag tattacctgc
    22261 atcaactcca atgactatat tcatagcttt aacatttgta gtaggtatat taggttacat
    22321 gggaaaaggt atatattatg cagtacaaga tgaagtaaaa gttccagtta aatattcagc
    22381 aactactata ggtatagctg cagctttagg attttcacca gatgtattcc aatttgcatt
    22441 aataggacac tggatagata cttatggcaa taaaggttac acatatacat ttatattcca
    22501 aatagcaatt ctagttattg gaatactatc ttgtttatat atattaaagg ttaaaaagag
    22561 aagagcaagt aaattagaag cttaaatatt caatttggta attaagtata tataaataca
    22621 atacttattt tgatgaatgt gattgtataa ataacataaa aaatatcaat agattaagtt
    22681 ttataataat gtttagagaa taaagtataa aaatcaaaat gttatcaagt tttctaggtt
    22741 ttaacataaa gttacccctg aaggtgtaaa attgagtggc aattattatc tattttctta
    22801 cctacataaa cctatttaat ttaaattcta agagattttc aaaatagatg gtagttgtac
    22861 tcaattttaa acctcataat aaatacgaaa atttaaaatc taactagata ctatttaatt
    22921 atatattaat aaatatatat tgtattattt atataataat aatttcaggc ttttcattat
    22981 atgtattcca atttatattg tttgattatt attcaaacac acgtcgttaa aaattttata
    23041 aatttatgtt tagatatgat taatactctt gattttagaa ataaatattt gtagattgta
    23101 gattaaagat aatataaata aatagaaggt agatttttta gatatattaa tataaagatt
    23161 atgtaaaata ttgaaatata gctgtttaaa ttattgtcta taaatataaa atgaatggtg
    23221 gtgagttaag tgaccgattt agttaacacc attaatggaa ttgtttggtc acccgtatta
    23281 gttgcaatgt gtttgtgtat aggattatat ttttcattga aattaaaatt ttttcaaata
    23341 agagatgtaa aagaaatgtt tactctcttg cttgaaggag atagttctca tgttggtata
    23401 tcatcatttc aaggttttgc tacatctatg gctggaagaa tagggactgg aaatatagct
    23461 ggtgtagctg tagcaatagc aatgggaggt ccaggagcat tagtatggat gtgtataatg
    23521 gctacattag gttcagcaac tgcatttgta gaatctactc tagctcaaat atataaagat
    23581 gagcatgatg ggcagtatag aggaggacct ccatattatt ttgaaaaagg tcttgggtgg
    23641 aaaaagtgtt cagttttatt ttcaatagtt gcaataataa gttatacatt ttttatgcct
    23701 ggtacacaat caaacacttt atatctagca atgaatcaag catttggatt ttcaaaaatg
    23761 acaatagcag tagcatcttc tattgcatta gcgataataa tttttggagg aattaaaagg
    23821 attggtttat ttgctgaaaa agtagtacct ataatgggtg gagcatacct tattatgaca
    23881 ttagtaataa ttataacaaa tattagttta gttccagagg ctattgtaac aatgtttaaa
    23941 tctgcatttg gatttggttc agtttttgga ggaatgctag gttcagcaat atcttgggga
    24001 gtaaaaagag gtatatactc aaatgaagca ggagaaggtt ctgggactta tggtgcggca
    24061 gcagcagaag taagtcatcc agccaaacaa ggattagttc aagcattttc tgtttatgta
    24121 gacacattat taatatgtat ggctacaggt attatgattg taataacagg aatgtataat
    24181 gttacaggtg tttgcacagc actgccaggt gtagaaccgg gaccacaata tgtacagagt
    24241 gcaataaata cattacatcc acaattaggt gatattttta tagcaatagt tttattatta
    24301 tttacattta caactttatt agccttctca tatatgatgg agactaatgt atcttactta
    24361 gtaactagaa ttaaagcaga aaaacatgaa aagtacataa ttaatgtctg tagatttatg
    24421 cttatagcat gtgcagcaat gtgttgtttt atagaaccac ttacaagctg ggcattaggt
    24481 gatatatgta taggtgtatt aacatatata aatttaattg ccatctttac actaaaaaaa
    24541 ccagctataa aggcatatta tgattatata agacagaaga agatgggaat aaaaaggagt
    24601 gatagaactt ttaaccctat agagttagat ataagaaatg ctgagttttg ggaaaataga
    24661 tacttaaaag aggataagca tgagttaaat taaagttata ataaaatata aaaattacta
    24721 aataaattta gtgggaggta agattggtat gaaagaaaga ataatacaag tggctgattc
    24781 aaagaaagaa aaaattctag ggctatgtca atctttatat gatgaaccag aaattgcttt
    24841 gcaagaatat aaatctgcta aaaaaatatc agaatttcta agagaagagg gttttgatgt
    24901 tgaggaaaac ttggcaggta tggctacagc ttttaaagca accaagaaaa atggagatgg
    24961 tcctaaaata gcatttatag cagaatatga tgctcttcca ggcaatggac atgcatgtgg
    25021 acatcattta attgcatcta tgggagttgg agctggaata gctctttcaa gtatactgga
    25081 tacttacaag ggagaagtaa gtataattgg aacacctgct gaagaaactg gagatggaaa
    25141 gccttacttg atagaacatg gtgtatttga tggctatgat gcagcaatga tgatacatcc
    25201 aaattcaaaa acttgtgtaa cacctgaaat tatagcaata ggaggtctag atttcatatt
    25261 tactggaaaa gcatctcatg caggagctaa gccatataac ggaataaatg ctttagatgc
    25321 agtagtacta ctatataata atataaatgc actaagacaa caattagtag atggaacgag
    25381 aatacatggt attatactag aagcaggaac tgctgcaaat gtaatacctg atatgggaaa
    25441 agtaagatta gagataaggg caaaagaaca aaattacttt gatgaagtag tagaaaaagt
    25501 gaaaaattgt gctagaggtg ctgcaatagc cactggatgt gaacttgaat tttatcactt
    25561 tgaaccaact tgccaaggat taaatgaaaa taaagttttg gtagacatat ttacaaagat
    25621 tatggaggaa tttggaatat atgaagatga acaagtaatg ttaggttcaa cagatatggg
    25681 aaatttaagt caaataatgc catgtataca tcctttgatg aagttttctg aaaatggaga
    25741 agaactacat acaaaagaat ttttggaggc ttctataaat tcatatgcaa aagatagagt
    25801 aattgatggt ataaaaatat tagctttaac aggatttaat ttatttgaaa atccagaatt
    25861 attgaaaaaa atgaaggaag agtagaactt agactatttt taattatact ctttaaaata
    25921 agaagagtgt ctcaaaatga actttttagt ttatgagaca ctcttttttg tatgaaatta
    25981 aatatatttt tattatttta aacatgaaaa agaggcttat ctgtttttat atgacctttt
    26041 ttaacttgta gcacaaaata tcatgaatga ttacaattcg aatgataaaa tcgattataa
    26101 gatatatagg aggggtacat atggaaactt tagaagtaag aaatattgaa ttgagtggta
    26161 caaattatga gataggatac agactaggag aattggttgc taatatgcca gagatactag
    26221 aaggtcagat taataaatct aatgtggtaa gcaaaaaaga agaaaaagag atgatagagt
    26281 tgtttgataa atattgtcca ggtcttaatg aagaattgca aggttttgct gatgcaattc
    26341 aagtgaattg taatcaaatt ttatattata ctatgactta tcttaaacca ggttgtagtc
    26401 aagttgcatt agcaccagaa ttaactgaaa atgggcatgt actatttgca cgtaatttcg
    26461 atttctcaca caatatggag gattttgttt tatgtaaaac taaggtaaat ggaaaatatg
    26521 cacatatagg tactaccgtt atgcaatttg gaagaggtga aggtatgaat gaatgtggct
    26581 taggtgtaag tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt ttaagaaaac
    26641 cagcaattgt aggtttacag ttttgggctg ttattagata tttgttagaa aattgcaaag
    26701 atgtagatga agcattagaa tatttaaaag atatgccaat tgcatacaat attaatttat
    26761 tgcttgcaga taagtctgga aacattgcac ttgtggagac tttagatggg aaaaaagaag
    26821 taaatataat caatagttta gaaagtaaaa gagaatattt tttacactct actaatcata
    26881 cacatattga taaacttcat aaattagagc cacaatcaat gaaaaattct attcatagat
    26941 ataaattaat taaagaatat ataaataagt ctaaaaaaat tggagagaaa gagttaatga
    27001 atttattgtc ttcaaaatat ccaaatggct tatcttgtaa ttactataat gatttttttg
    27061 gaacattaaa aagtatagtt atggatttaa atataggaaa gtttaatatt ttatggggtg
    27121 gactagagaa taaatgggag agctactatt taaaaaatga cattaaatct ataacacaga
    27181 ggataaacat aaatattgaa aaagcaccat ctgacttttt tgatttcatt gaataaaaaa
    27241 tgtatttaat tattctaaat aattaggata tataaatcaa ctaaaagttt ttatttatcg
    27301 tattgaatta gttataatat tttagtatag taaaaaatag tgctatttta catatttaaa
    27361 gagtttaagt agaagagagg tgccatattg gattacttaa agtattttga aaaagctgta
    27421 atgtatattg agaataattt acatgaaaat ataactgtaa atgatgtggc aaaagaaact
    27481 ggatattctt attatcattt aacacgccta tttaagtcta tgtttggtga gagtgtagga
    27541 agttatataa aaaaaagacg attagtaagt agtacaaaag aacttttata tagtgataaa
    27601 aaagtgatag atatagctat tggtagtgga tttgaatctt cggaagcatt tagcagagca
    27661 tttaaatcta tatacaaggt tagccctata gaatatagaa aaaatagaat tgatgtattt
    27721 gttggtaaaa agaaaaaatt agagttagat tttatgagac atcttgttgg aaatataacg
    27781 attaaaccag ttataaaaga aatagaaaaa ataaaggtaa taggtataaa ggataaagta
    27841 attttagaaa ataattcatt acctgacttg tgggagaaat tcagaaaagt acatcatatt
    27901 gtcccaaata ctttgccatc aaagagagtt tttggaatct gtgaagcaat ctctgaaata
    27961 cacctagtta gtgaaagtat ggaatttaat gagataatag gacttgaagt taatagttat
    28021 gatgtgattc caaattcatt tgtttcaaaa actattaagg aaggaaagta tgctatattt
    28081 actcatactg gtagcttaga taatttagac aaaacttatg aatatatatg gggtacttgg
    28141 tttttaagtt caaaagaaga acttgatata agagatgatt ttgaagtata tgatgaacga
    28201 tttttaggtc ctagtaatgt gaactcacaa attgatattt atatacctat aaaataatct
    28261 ataagaaaac taaaagataa tttgattaag aaatagatgt tcgtaaaaga gcatctattt
    28321 tt
//
LOCUS       DAFKMX010000030        28268 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFKMX010000030 DAFKMX010000000
VERSION     DAFKMX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.030"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..481
                     /locus_tag="LZB08_001084"
     CDS             <1..481
                     /locus_tag="LZB08_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBM7314597.1"
                     /translation="VTKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPL
                     HKKRLNKRGFNQAQKIAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFV
                     VKENVKLINNKNILLIDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            813..2726
                     /locus_tag="LZB08_001085"
     CDS             813..2726
                     /locus_tag="LZB08_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7314598.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            2867..3175
                     /locus_tag="LZB08_001086"
     CDS             2867..3175
                     /locus_tag="LZB08_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7314599.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            3199..3525
                     /locus_tag="LZB08_001087"
     CDS             3199..3525
                     /locus_tag="LZB08_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314600.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            3564..4898
                     /gene="celB"
                     /locus_tag="LZB08_001088"
     CDS             3564..4898
                     /gene="celB"
                     /locus_tag="LZB08_001088"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBM7314601.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGNVHEFIFNFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFDLPIMLNPLLLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKILDKQYLKDEKSNVAEEDDDISFDDLVL"
     gene            4909..5427
                     /locus_tag="LZB08_001089"
     CDS             4909..5427
                     /locus_tag="LZB08_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBM7314602.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMDPFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            5523..6482
                     /locus_tag="LZB08_001090"
     CDS             5523..6482
                     /locus_tag="LZB08_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBM7314603.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            6531..7583
                     /locus_tag="LZB08_001091"
     CDS             6531..7583
                     /locus_tag="LZB08_001091"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBM7314604.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKSVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KFQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            7586..8260
                     /locus_tag="LZB08_001092"
     CDS             7586..8260
                     /locus_tag="LZB08_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBM7314605.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKESIVHNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            8813..9364
                     /gene="raiA"
                     /locus_tag="LZB08_001093"
     CDS             8813..9364
                     /gene="raiA"
                     /locus_tag="LZB08_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBM7314606.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            9496..12171
                     /gene="secA"
                     /locus_tag="LZB08_001094"
     CDS             9496..12171
                     /gene="secA"
                     /locus_tag="LZB08_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBM7314607.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            12186..13293
                     /gene="prfB"
                     /locus_tag="LZB08_001095"
     CDS             join(12186..12260,12262..13293)
                     /gene="prfB"
                     /locus_tag="LZB08_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBM7314608.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            13437..15578
                     /locus_tag="LZB08_001096"
     CDS             13437..15578
                     /locus_tag="LZB08_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBM7314609.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            15726..16892
                     /locus_tag="LZB08_001097"
     CDS             15726..16892
                     /locus_tag="LZB08_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7314610.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEIGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      16952..17069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            17194..17799
                     /locus_tag="LZB08_001098"
     CDS             17194..17799
                     /locus_tag="LZB08_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBM7314611.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            18128..19168
                     /locus_tag="LZB08_001099"
     CDS             18128..19168
                     /locus_tag="LZB08_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBM7314612.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            19307..19759
                     /gene="tsaE"
                     /locus_tag="LZB08_001100"
     CDS             19307..19759
                     /gene="tsaE"
                     /locus_tag="LZB08_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBM7314613.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            19759..20475
                     /gene="tsaB"
                     /locus_tag="LZB08_001101"
     CDS             19759..20475
                     /gene="tsaB"
                     /locus_tag="LZB08_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBM7314614.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYSRNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            20465..20941
                     /gene="rimI"
                     /locus_tag="LZB08_001102"
     CDS             20465..20941
                     /gene="rimI"
                     /locus_tag="LZB08_001102"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBM7314615.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            20947..21963
                     /gene="tsaD"
                     /locus_tag="LZB08_001103"
     CDS             20947..21963
                     /gene="tsaD"
                     /locus_tag="LZB08_001103"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBM7314616.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVIQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDKLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            22260..24968
                     /gene="hpdB"
                     /locus_tag="LZB08_001104"
     CDS             22260..24968
                     /gene="hpdB"
                     /locus_tag="LZB08_001104"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBM7314617.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIKMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            24972..25229
                     /gene="hpdC"
                     /locus_tag="LZB08_001105"
     CDS             24972..25229
                     /gene="hpdC"
                     /locus_tag="LZB08_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBM7314618.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            25222..26172
                     /gene="hpdA"
                     /locus_tag="LZB08_001106"
     CDS             25222..26172
                     /gene="hpdA"
                     /locus_tag="LZB08_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBM7314619.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYGNGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWTQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            26488..27450
                     /locus_tag="LZB08_001107"
     CDS             26488..27450
                     /locus_tag="LZB08_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7314620.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(27549..>28268)
                     /locus_tag="LZB08_001108"
     CDS             complement(27549..>28268)
                     /locus_tag="LZB08_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7314621.1"
                     /translation="LIILFFLIYSLLIVLKLLNLINNNEFIIINLILTFISSLSIIIY
                     SSNKKNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYSGIIKFIFTPITSL
                     TTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPLIFTLYTPEAPILG
                     IILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSKESTITYHDILIVS
                     MVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        11496 a         2932 c         5253 g         8587 t
ORIGIN      
        1 tgtgacaaaa aagatggtgt tagggctgaa gtataaccaa aaaaccttta tggctaagta
       61 tatagctcaa ataatgaaag aaaaattata tttagaaaac ataaaatttg attatatatt
      121 atttgttcca cttcataaaa aaaggctaaa taaaagaggt tttaatcagg cacaaaaaat
      181 agcatttaat ttaagtaaaa tagtaaatat tccattatta gattgcataa gtagaaaaaa
      241 atatacaaga atgctatata aattgaataa gaaagaacga aaagaagaat taaaaaatgt
      301 atttgttgtt aaagaaaatg ttaaattaat aaataataaa aatatacttt taatagatga
      361 tatatttact actggtctaa caacaaacga aatttctaaa cttttaaagt tatcaggtgc
      421 taataaggtt tttgtattga ctctacttac aaaagcaaat gataattata ttatggaata
      481 atcttatata ttgacaattt tagtttaaaa tattaaaatg aataagtgat atttaggtct
      541 gtggttgaaa gtccaagcca gtcgcaggca aagggatcca cgtaagttaa cactaaaaat
      601 tgggttaatg atcatggtgc ggcttagaag taagacctac cgataataaa atcgagagag
      661 agtagtagtg cggcgaaccc aagcgaaccc tccagtaggc gagtgtgggg tcaaaaacca
      721 ggtcagctaa atatcatatt aaaagagagc tagcatacct ttatgtatag ctctttttta
      781 tatataataa atttaggttg agtcataaag aggtgatatg tttgaataaa agacaagaaa
      841 atttattaac aacattgcta ttaaaagaag agtttataaa tgttaataag attgccaatg
      901 attttgagtg ttcagaaagg acaataagaa atgattgcaa atatattgat gaatggttat
      961 ctactttttg taatgcttat atcaaaagaa aacctaatat tgggattaaa tttagtggag
     1021 atggaacaga caaaatactg gtaaatcaga agttgcgagg tgaagttaaa agagtaaata
     1081 gtgagcttca taagcaagtt gagatattaa atttactact ttgtaataat aaaaaagtta
     1141 ctttaaatga tttatctaat aagttgtaca taaataaaaa tattgtcaga gaagaaataa
     1201 gtaaattatc agtagcatta aataattata atttaaatat ctcgactaaa aaaggcagtg
     1261 gtatatatat tgaaggggat gagaaagaca taagaataat gttagtatca ttcttattta
     1321 aatatataga taaatacaga ttaaacatag atgaaataga gtacttccat atagtagata
     1381 taataattgt aaaaaacata atgcttacta tagaggagga tatggatata agacttacag
     1441 atatatcatt caagcaattg atgctatttt tgcttataaa tataatacga ataaggctgg
     1501 gaaattcttt aaaagtatca aatactaaga aagagcttaa gctaaaaatg cttatattgg
     1561 atatagaaga tgaattgaaa tctaatttat ccatagtatt aaattatgaa gaaaaattgt
     1621 ttatagaatc tttaatattg ggtatgaaca aacaaataaa tagtaataat attaaaaaat
     1681 ctttatatct aaatgaagaa ttagcaagtt atacaaaaaa aattataaat ttagtatcag
     1741 aagaatcagg aataaatttt aataatgata tattacttta tgaacaatta atatgtcatt
     1801 tgaatgttac aatgcatcaa atgaaaagca atgtttactt agaaaatcca cttttagata
     1861 acataaaaac taaattttgt tttttattta cagtaatatc aagttcaata gaaattgaat
     1921 taaaaggaaa acaaataacg gaagatgaaa ttggttatct tacacttcac tttcaaacct
     1981 ctctagaaag aaaatacagc aaaagggaat caaataaaaa ggcaagtatt gtatgtccat
     2041 ttagttttgg agtaagtatg ctattaaaag taaagataga aaagagattt gataacataa
     2101 aaattattga aacattaaga gaggaagatt taaaaagaga taactttaac aagactatag
     2161 attttataat tgcatttcaa gagtatgaaa atatccaaaa accaattttt ataacaacac
     2221 cattatttac tgatgaggat gagaataaac ttaaagagtt cacaaacaaa ataaaagaaa
     2281 aagacacttc atataaactt atgaacagat taatgatgtc tgattattta ataagagagt
     2341 tagaacatga tgatatttat gaagctataa catatttaac taatttatta attgaaaaac
     2401 attatgcaga aaaagagtat ttacaatcta ttataacaag agaaaagtca tatccaacca
     2461 atgttggaaa aggaatatta ttccctcatg gagacatgaa atatataaaa caatctgttt
     2521 tatgttttgc aaggttaaaa acaccaataa aaattagaaa tggaaaggat ataaagatta
     2581 tacttttgtt agcatataaa aaagatgatg atagaaaagt atttagagaa ttatttaaag
     2641 aaatatctaa tttaacagag gatgaaaata tgctagatat tctaagtaaa tgtgacattg
     2701 aaaaggtaaa agatattctt atataaaatt catagtgaac tatggataag cacaaaattt
     2761 tccatgtgat ggaaaaagtt gtgcatgttt ttttgttttt ataagatatt atatgtttgt
     2821 aaggtataaa taatctaaaa gtagagaagc aacttaggag gaaaaaatgg aagaaatagt
     2881 tttaaagatt ataattcatg ctggaaatgc aaaatctatg ctttatgaag cacttgacta
     2941 tgcaaaggaa aacgatttta aaaaagcaga tgaattaatt gaaaatgcaa atgaagaaat
     3001 tttaaaagca cataaagtac aaactgaact tatacaaaag gaagctgggg gagataaatc
     3061 agatatatct attttattga ttcactctca agaccattta atgacttgta tgagtgagag
     3121 aaaccttata aaagaaatga ttatgttaag aaaagaaatt caaaaattaa aataaaaaaa
     3181 ttaaggggga tttaaaaaat gaaaagaaaa gtatatttat tttgtagctt tggtatgtca
     3241 acaagtttac ttgcagataa aatgcagaag gtagcagatg aacacaattt accaatagaa
     3301 gtagaggcat ttccgatagc agaaattgac aaaatagttg aagaaaaaaa tccagattgt
     3361 atattattag gaccacaagt taaatacatg ctaaaggagt taaaaccaaa gtttgaagct
     3421 caaggaaaac ttatagatat aatcaatgaa gttgattatg gaactatgaa tggggaaaaa
     3481 gtattaaaac ttgctataaa gctaataaaa tctaaacaag cgtagaatta tagtaaaaaa
     3541 taataggaaa tgagggtgga tagatgaata gattggaaag aatacttatg ccattggctg
     3601 aaaaaatagg taaaaataaa ttattaattg caattagaga tggtttttta gtatcatcac
     3661 cattattaat aataggttca ttatttttat tagtagcaaa ttttcctata aaaggatgga
     3721 atgaatttgt aggtcaaatt tttggtccag attgggctgt aaaattgcaa caaccaactg
     3781 tagcaacatt tgaagtaatg actctacttg cagttttagg gattggatat tcttatgcaa
     3841 agcaaataga tgtagaccca atagcatcag cagcagttgc aatagttgcc ttttttataa
     3901 taacaccatt tgtaatacca tatacaccag aaggaacaga atcagtttac atggtaacag
     3961 gtataccact aggttggatg ggctctaagg gaatgtttgt aggtatgata acagcaataa
     4021 cctctgtaaa attatttgct gcagttgtaa aaaaaggatg gacattgaaa atgccagatg
     4081 gagttcctcc aaccgtatca aaatcatttg cagcattaat accaagtgca atagttatgg
     4141 tagttttctt tttagttaag atagcttttg aagctacacc atatggaaat gtacatgaat
     4201 ttatatttaa ttttttacaa ataccattat taaaattagg aaatacttta agcgctacag
     4261 caattgctta tgtattctta catctattct ggttctttgg aataaatgga agtagtgttg
     4321 ttggagctgt gtataatcca atattaaaaa ttctatcagc tgaaaactta gctgcttttc
     4381 aagctggtac aaaactacca aacataataa ctgctcaatt tcaagatatg tttgcaacat
     4441 ttggtggggc aggtagtaca ttatcattaa ttatagctat gttattaata tgtaaaagta
     4501 aacgtataaa atcaatagga aaattatcaa ttttaccagg tatatttggt ataaatgagc
     4561 cattgatatt tgatctaccg ataatgctta atccattgct attgatacca tttgcattag
     4621 ttccaacagt aaatataata atagcttatt tctgtatgtc aacaggatta gtaccactta
     4681 caaatggagt tcagctaccg tggacaactc caattatatt ctctggattt ctaacaacgg
     4741 gatggcaagg ggcagtttta caattagtac ttcttatact aggaatattt atgtatatac
     4801 catttataaa aatactagac aaacaatatt taaaagacga aaaatcaaat gttgcagagg
     4861 aagatgatga tatatctttt gatgatttag tattatagga ggaagattat gaagaaaata
     4921 gtgatgatat tggaccaaat acaagctgga gctggaggaa aagagaaaag taatatacct
     4981 cctgctggaa aatcaagccc attaggacca ggtgtaatga tggatccatt tttaaatgaa
     5041 tctaaggtaa tagctacctt attttgtggg gatgaatttt ttgtaaataa tcaagaagaa
     5101 gtaacgagta agatgatagc tatggtaaaa aaattaaacc ctgatgtagt aatctgtgga
     5161 ccaagcttta attacgaaaa cttttctaag atgtcagcaa tactgtctaa aaatataaat
     5221 gataaaacag atataccagc ttttgcagct atgtcagaag aaaatataga tgtgataaac
     5281 gaatataaaa atgacatatg tatagttaag actccaaaaa aaggtggaat tggtcttaat
     5341 gattcattaa ataacatatg taaattggca aaagcaatag caaataaaga agatataact
     5401 ttgatgaaag aagaattttg ttattaataa gagtattaat tattagcaag tttaaatatt
     5461 atactattta taataagcat acattagtaa aatctatata atttaaggag gggaatttta
     5521 acatgtgggg aatgatagcg acatggagaa tggccctaga aggaataact gaggcatcag
     5581 aacatttaga aaaaggcatg gaagcaggtg atgctgtaga gcttgctgta agaagagttg
     5641 aagactatcc attctataaa tcggttggat atggtggact tccaaatgaa aattgtgagg
     5701 tagaacttga cgctgcattt atggatggta acacattaga tattggagca gttgctggta
     5761 taagagatta ttctaatcct gtcagcatag ctaggaagtt aagttatgag aaggtaaata
     5821 attttttagt tggaataggt gctgaagatt atgctcataa aaatggattt gaaagaaaaa
     5881 atatgttgac tgatagagcg aagttacatt ataaaaagag aaaaaaagag actttagaca
     5941 aagggttaag cccatatgct ggtcatgata ctgttggtat gatatcttta gacaaaactg
     6001 gaaaaatgtg tgcagctaca tcaacaagtg gattgtttat gaagaaaaga ggtagaatag
     6061 gagattctgc actatctggt tcaggattct atgttgatag tagtatagga ggagctacag
     6121 ctactggttt gggagaagat ttaatgaagg gatgtatatc ttatgagata gttagactta
     6181 tgaaagaagg gaagcatcca caagaagcat gtgatattgc agtaaatgaa cttgataaac
     6241 agcttatcaa aagaagagga aaagctggtg atttatcatt agttgccatg aataataaag
     6301 gtgaatgggg agtagctaca aatatagctg attttacttt ttcagtagta actaaagaag
     6361 aatctcctac tgtatatatt tcaaatagag tagatggaaa gactatacat aaggttatat
     6421 caaatgagtg gatggaagca tatgaggcta gaataaaagc accaatagaa gtaagtgagt
     6481 aaagtagtgg ttacataatt aaaatagagt tttataataa ggagattttt atgaatatca
     6541 taagtaataa aatagatgag ttaaaagaag atttattaag tgatattata gatattgtaa
     6601 aaatacctag tgtcaaaggg gagtcagaaa atggcttccc ttttggcgaa aaagtagggg
     6661 aggcacttaa taaagcatta gaaatatcag aaaaattggg atttaaggtt agaaacttgg
     6721 ataattatat tggttatgca gagcatggag atagtgatga ttatgtttgt gtaattggac
     6781 atgtagatgt tgtacatgaa ggagatggtt ggaagcatca gccttataag ggagaagaaa
     6841 ctaatggaag aatttatgga cgaggagtac tagacaataa aggacctata atgtctgcct
     6901 tatatggatt atatgctatt aaagaattaa acttaaaatt agataagagc gttagaataa
     6961 tatttggtac aaatgaagaa agtggatttg aagatatacc atattattta gaaaaagaaa
     7021 aagcacctat tatgggattt acaccagatt gtaaatatcc tgttgtatat ggtgaaaaag
     7081 gtatggcaaa gattagaata aaaagtaaaa tcaattatga agaagatgta tacctaggtt
     7141 ttatagaaaa tatgtctgaa aatgtgcttg ttacatataa agagctgaat atagaaaata
     7201 gtgatactat attagatata aaggtaaaat acgattttag ttataaattg aaagatgtat
     7261 tagatgaaat aaaagctagt tttccaaata gtatcgatat agaggtaatt tctaatttta
     7321 atcctgtata ttttgataaa gagagtaatc ttgttaaaaa gtttcagcta gcttatgaaa
     7381 gggttacatc attagatgga actccagtaa caactaatgg aggtacgtat gcaaaagtaa
     7441 tgccaaatat agttccgttt ggaccttcat ttccaggaca aaaaggaata gctcataatc
     7501 ctgatgaata catggatata gaagatataa tattaaatgc aaagatattt gcaaatgcaa
     7561 tatatgaatt agcaaaggag tagttatgtt agaaataata ggaatgtcag tagaagacgc
     7621 taaaattata gaagattgtg gtgcagatag aatagagtta gttagtgctt taactgaagg
     7681 tggattgaca ccaagttttg gtcttataga aagtgttgtt aacagtgtaa agatacctgt
     7741 gaacgtaatg ataagacatc atgcgaaaag ttttgtatac agtaaagaag atataagtat
     7801 aatgcaaaaa gatataagtg tggttaaaga gattggtgct aatggagttg tatttggtgt
     7861 attggataaa aacaacaata tagatgagaa aaatctaaat gttttattga aatgttgtga
     7921 taatcttgat gttacatttc atagagccat agatgaatca aatacaatag atagtgttaa
     7981 aattttgaag gattatgata aaattacgaa tatattaaca tcaggtggga aagaaagtat
     8041 agttcataac atacaaatga taaaaaatat gatgcttagt tctaatcaca taaaaattct
     8101 tttaggtgga ggtcttaatt ttaataatat agaaaagata aaggaattaa ctaaggcaag
     8161 taattttcat tttggtacag cgattagaat aaataacagt ccttttgaag atatagatag
     8221 acaaaagctt aaacaactcg taaatataat ttcaagatag tatatgaata taaaatactt
     8281 agtttaaata aagagagtaa gttaattata atttgggtat atgaatgtta ataggtatta
     8341 aatatgaaat tattgttgag tatgctttat aacttttaag tagttttaaa tataaataat
     8401 gaaagaaaag atagattatg gaggttttag agggtttata tttattaaaa aattataaag
     8461 gtatattggc atataataca cataatttcc taaaattacc atcttatgac atgaattcac
     8521 atattaataa acaattaatg gagatttttg tggatagaaa taggagtaaa aatagcttta
     8581 aataaacaaa aacaagagtt attaacagtt ttatccacat tatccacagg tgtttgtaat
     8641 aatattaatc cacataagta tcaatgtaac taaaaatgat aaataattct atcaattgaa
     8701 tagaaaaaat cataaaagta aataataaaa agaggattat gagagttcgt gtagaatata
     8761 ttattaaagt ataaattttc ttatactggc ataattgaag ggagatgtat ttatgaacat
     8821 aattatatct ggaaaacaaa tcgaattaac tgatgcaata aaaaataaaa tcgaaactaa
     8881 actaagtaag ttagataatt acattcatcc agacactgat gtaaaagtta cagtaagtgc
     8941 gagaaaagca agacaaaaaa tagaagtaac aatagctact gtaaatggtc ctataattag
     9001 agcagaagat atgcaggaaa atttatacac agctatagat gttgtatatg ataaattaag
     9061 taaacaatta aaaaaatata aaaagagatt acaagataaa cataaagaca acaagagtat
     9121 aagattccaa gatgcagaga taaatctagc tgatgaacaa gaggattatg gggatgaaaa
     9181 tcaagaatta gtaattcaaa gacgtaagaa atttagtgtt aagccaatga gtgaagaaga
     9241 agctgtatta cagatggaat taataggaca tgatttttat atgttcaaga acatagattc
     9301 agatgaaata gcagtagttt ataagcgtaa taatggtgga tatggtataa tagaacatga
     9361 ataaagcaat ttagatttaa tcatattagc tatcgataaa taaattatcg atagcttttt
     9421 aaattaatat taaaagtata gtttttatgg taaaatgaat aatagtacaa taaagaacga
     9481 ggagagatgt aaaatatgag ctttatggat aatttattca acatggcaga taaaaaagaa
     9541 ttaaaaaagt tcaataaaac tgttgatata atagattcat tagaacctaa atttgaatct
     9601 atggcagact cggaacttaa aaatatgaca aatatattta aagaaagatt agcaaatggt
     9661 gaaagtatag atgatatact tccagaagct tttgcagtag ttagagaagt ttctaagaga
     9721 gtgttaggac ttagacatta cagagttcaa atgattggag gtatagtact acatcaagga
     9781 agaatagcag aaatgaaaac aggtgaaggt aaaactttgg ttgcaactgc tcctgtttat
     9841 ttgaatgcgc ttactggaaa aggtgtacat gttgtaacag taaatgatta ccttgcaaaa
     9901 cgtgatagag accaaatggc aaaaatctat gagtttttag gaatgagtgt aggcgttatt
     9961 atacacggtc aaaatccaaa agttagaaaa gaacaatatg attgtgatat aacttatggt
    10021 acaaataatg aatatggatt tgactatcta aaggacaata tggtaataca caaagagcaa
    10081 agagttcaaa gaggattaaa ttatgctata gtggatgagg ttgactctat actaatagat
    10141 gaggctagaa caccacttat catatctgga cctggagaca aatcaactca cttatattca
    10201 gatgcaaaca catttgtttt aaccttaaaa ccagatgatt atgaattaga agaaaaagat
    10261 aaggctgtat ctctaacagc atcgggtata caaaaagcag aagtgtattt taatgttgat
    10321 aatataacag atatatctca tacagaatta taccatcata taaatcaagc tttaagagca
    10381 catgtaataa tgaagaaaga tgttgattat gttgctaaag atggagaaat agtaatagta
    10441 gatgagttta caggtagact tatgtttggt agaagatatt cagaaggttt acatcaagca
    10501 atagaggcta aagaaggtct taaaatacaa agagaatcta aaacactagc tactgtaaca
    10561 ttccaaaact actttagaat gtacaaaaaa ctttctggta tgactggtac agcaaaaaca
    10621 gaagaagaag agtttaaagc aatctacaaa atggatgttt tccaagtacc tactaataag
    10681 cttatgataa gggaagactt accagattgt gtatacaaaa gtgaaatagg taaatttaat
    10741 gctgtagcac aagaaataat tgaaaggcac aaagtcaatc aaccaatact tgtaggtaca
    10801 gtatcaatag aaaaatcaga attattatca caaatactaa agaaaaaggg cataaagcac
    10861 gaggtattaa atgctaagca tcatgataaa gaggcagaaa taatagcaca agctggtaga
    10921 cttggagctg ttacaatagc tactaatatg gctggtcgtg gtacagatat agttctaggt
    10981 ggaaatccag actttttaac taagagagaa atgagaagaa atggatttaa agaagaaata
    11041 gtaaatagag tagatactcc aatagaaggt atacctgtaa aaggtaatga aattctattt
    11101 gaagctagag aagagtatga aaaactattt gaaaagttta agcaacaaac acaagaagaa
    11161 caaaaacaag ttgtagaagc aggtggactt gctataatcg gtacagaaag acacgaatct
    11221 cgaagaatag ataaccagtt gagaggtcgt gctggacgtc aaggtgaccc agggagttca
    11281 agattctata taggtcttga tgatgacctt atgagattgt ttggtagtga tagaatatct
    11341 ggtattgtag ataaaatagg tcttgaagaa gacatgccga tagaacatag aatattatct
    11401 aagtctattg aaggagcaca aaagaaagtc gaaggtaaaa actttggaat aagaaagcat
    11461 gtacttcaat atgatgatgt tatgaataaa caaagagaaa taatatatgc agagagaaaa
    11521 cgtgtattag aaggtgaaga tttacaagaa caaatacaat caatgactca ttctataata
    11581 gaagaagcag ttactcttta tacacaagat aaaggatttg atgaagaagg attcaaagaa
    11641 catatgtata atttgttctt gccaaaaggt agcatagaaa taccagaaat agaaaaactt
    11701 aatccagttg aaataacaga aaaagtttat gaaatagcta tgaagatata tacttcaaag
    11761 gaagaacaag ttggctatga gagaatgaga gaagttgaga gagtaatctt acttcaagct
    11821 gttgataacc attggataga ccatatagat gctatggacc aattacgcca aggtattggt
    11881 cttcgtgctg taggtcagca agacccagtt attgcttata aaatggaagg ttttgatatg
    11941 tttgatgaaa tgaataaaca tatcaaagaa gatactgtaa gatatttatt taatataaca
    12001 atagaaactc cagtggaaag aaaagcagtt gtagatgttg aaaatctatc ttctccatca
    12061 gatggaacac ttccaacaag taaaactgtt aaaaaagatg aaaaggttgg aagaaatgac
    12121 ttatgccctt gtggaagtgg taaaaaatat aaaaactgtt gtggaagata atctcggagg
    12181 attaaatgtt aaagttacaa gagtatagac atagtatgga gcagatggct tcaaatttag
    12241 aagaattgag ggtttctctt tgacatagct tcattgatga aaaatataca aattaatgaa
    12301 gaaaagatga atcaacaaga cttttggaat gataatgaag tcgctcaaag agtacttcaa
    12361 gaaaataaat ctttaaaaga gacattagaa gaatatgaaa gcttaaaaag cttattagaa
    12421 gatatagaag tattaattga aataggttta gaagaagatg atgattctgt agaaagagat
    12481 atagaaaagt ccatagaatc aatggaagaa aaattatctg aaatgaaaat aaagactctt
    12541 ctaaatggag agtatgataa aaataatgct atattatcta taaatgctgg tactggtggc
    12601 ttagatgctc aagattgggc tcaaatgctt ttgagaatgt atataagatg gtcagaaagt
    12661 aagggttata aagttaagct tttagatata atatcagacc ctgaagcagg gataaagaca
    12721 gctacaattc ttgtagaagg aacaaatgct tatgggtatt taaagagtga aaaaggagtt
    12781 catagattag tgagaatatc tccatttgac ccatcaggaa aaagacatac ttcatttgca
    12841 tctatagatg taacaccaga gttagatgaa aatatagaag tagaaattaa tccatcagat
    12901 ttaaagatag acacatatag agcttctgga gctggagggc aacatgtaaa tacgactgat
    12961 tcagcagtta gaataacaca tatccctact ggtgtagtag ttcagtgcca aaatgaaagg
    13021 tctcagcatc taaataagga cagagctatg agacttttaa tggcaaaatt gatagaatta
    13081 aaagagttag aacaaaagga aaaaatagaa gatatacaag gcaaatactc acaaataact
    13141 tggggtagtc aaattaggtc atatgttttt caaccatata aattggttaa ggaccataga
    13201 acaaatgctg agtttggaaa tgtagatagt gtaatgaatg gaaatataga cttgtttatt
    13261 aatgaatact taaaaatgaa taaaatagtt taataaatat gcactgtaat taagatatat
    13321 gtataagaag gttagtgctt aatatgcact tacagcactt aggctgagga cgataaccct
    13381 cagccttcgt tctgttttgt tatacttatg aaaaactaac attaggagtg aataaattgg
    13441 atataaatca aatcttaaaa aaagagttta atcttagaga tgaacaaata aataatacat
    13501 taaaacttat tgatgaagga aatacaatac catttatagc tagatataga aaagaaatga
    13561 ctggcgaaat gagtgatgtt acactaagag aattttatga aaaattaatg tatttgagaa
    13621 atcttcaaag tagaaaagat gatgtagtta gactaataga tgaacaaggg aaattaacag
    13681 atgaaataac acaaaatata gaaaaagcaa aaacattaca agaagtagaa gatatatatg
    13741 ctccatataa acaaaagaaa agaactagag caacaatagc aaaagaaaaa ggattagaaa
    13801 atttagcatt gtcaatacta gagaataatt tagataatat agaaatagag gcaaagaatt
    13861 atcttgatga agagaaagaa gttctttcta ttgaagatgc tctaaaaggt gctagagata
    13921 taattgcaga actagtctca gatgatgcta agattagaaa gtatataaga gaattagctt
    13981 taagagaagg tatgatagtt agcaaaagtg ctactgatga gaaatcggtt tatgatatgt
    14041 attatgatta tagcgaagca gtaaaaagta tggctccaca tagagttttg gcaataaaca
    14101 gaggagaaaa agaaagcttc ctaaaggtta agttggaaat aaataatgat aaagttttaa
    14161 attacataat aaacgaatat gtaaatgata aaaatttcaa aaataaagaa gaaatcgtaa
    14221 gttcaataga agattcttat aaaagattaa ttttcccatc aatagaaaga gaaataagaa
    14281 atcatttaac agaaatagct caagaaagag ctataagtgt atttggaaag aatgttaaaa
    14341 gtttattgct tcaaccacca gttaaagaca aagtagttat gggatttgat cctgcattta
    14401 gaactggatg taaaatagct gtagtagata aaaatggtaa attactagat tatacaacag
    14461 tatatccaac agaccctcaa aatgatgtag agggagcaaa gaaagtttta aaaggattga
    14521 ttgaaaaata cgatatagat ataatttcaa ttggaaatgg tactgcatct agagaatcag
    14581 aaacttttgt atcagaaatg ataaaagaaa tagatagcga agtgcagtat gtgatagtta
    14641 gtgaagcagg agcatctgtt tattcagcat ctgaacttgc aaatgaggaa catccagata
    14701 taaatgtatc aataagagga gctatatcta tagctagaag attacaagac ccattagcag
    14761 agcttgtaaa aatagaccct aaaagtattg gtgttggtca atatcagcat gatttaaaca
    14821 aaaagagatt agaagaagta ctagatggag ttgttgaaga ttctgtaaat agtgttggag
    14881 tagatttaaa tactgcatca tactccctac tagaacatgt tgcaggaata agtaaagcta
    14941 tagcaaaaaa tataatagca tatagagaag aaaatggaga ttttacgtca agagcacaat
    15001 tgaaaaaagt aaaaagacta ggacctcaag catttactca atgtgctgga tttatgagaa
    15061 tcttagaggg taaaaatcca ctagataata caggtgtgca tcctgaaagt tatgatatat
    15121 gtaaaaaaat gattgagata attggatatt ctttagatga tgtaaagaat aagaatatag
    15181 gtgaaataga tgaaaaaata aaagaaattg gcttgaggga attaagtgaa aaactggaag
    15241 taggacaagt tacgttaaaa gatatcatag ctgaaatcaa aaaaccagga agagacccaa
    15301 gagaagaagg tataaaacct atacttagaa ctgatgtctt aaaaattgaa gatatacaag
    15361 agggtatgac tttaaaaggt acaataagaa atgttgtaga ctttggtgca tttgtagata
    15421 ttggaataaa aaatgacgga ttagttcata aatcagaaat gagtaatagt tttgtgaaag
    15481 accctatgag tattgttaca gtaggtgata tagtagatgt aaaagtcatt ggaattgact
    15541 taaacaaaaa aagagttgcc ttatcaatga aaaaatagat aagtttatgt atggttatag
    15601 tacttaaatt ctatatttgt aaatgagatt actcgagtgt ataaatgtta aaaatttgta
    15661 aaagtatata gtaagaaaat atattaaaat aagaacgata gtcttgaaaa tcaggaggaa
    15721 aatgaatgat ttttataaaa aatggtaaaa taaataccat aacaaatggg ataattcatg
    15781 gagatatact tatagatgaa ggaaaaataa tagaaattgg agaagatttg atagctcctt
    15841 tagatgtaga ggtaatagat gctagtaata aacttgtgtt tcctggattt atagatgctc
    15901 atactcactt aggtttatgg gaagatggta tagggtttga aggagcagat ggaaatgaag
    15961 agactgaccc aataacacca caattaaatc ctatagatgg aattaatcca atggatagaa
    16021 cttttaaaga agcttttgaa ggtggaataa cttctgtttg tacaacccct ggaagtgcaa
    16081 atgttatggg tggtcaatgt atagctataa agacatgtgg aaaaagaatt gataaaatgg
    16141 taataaaaaa tccagttgca tctaaaattg cttttggaga aaatccaaag agctgttatg
    16201 gtcaggatga taaatctcct caaactagaa tggctatagc tgctttactt agagaaaacc
    16261 taaaaaaagc agaagaatat ttagaggata ttgatatgta tgagagtcat gatgatgaag
    16321 actgtgaaaa acctgaatat gatataaaaa tggaaagttt aataccagtt ttaagaagag
    16381 aaattccttt taaggcacat gctcatagag cagatgatat gtttacagca ataagaattg
    16441 caaaagaatt taatttaaag cttacattag accattgtac agaaggtcat cttattgtag
    16501 atgaattagt ggaagaaggg tttcctgtaa tagtagggcc atctttgtca gaaaggtcta
    16561 aatttgagct tagaaatttg acatttaata cagcaggaat actttcaaat gcaggtcttg
    16621 atgtatgtat aatgacagac catccagtaa taccagttca atatcttcca atatgtgcag
    16681 gtattgcagt aaaacatggt atgaaagaag aaaaagcaat agaatcaatt acgataaatc
    16741 cagctaaggc tttaggaata gaagatagag taggctctat agaaattggt aaagatgcag
    16801 acctagtaat ttgggataat tctccactag agattcaaag taatgtactt tacactataa
    16861 taaatggcaa agtagtgtat gagaaaaaat aacttgacaa ttattaaaaa attaactaat
    16921 ataagagtgt aaggaaaatt aaaaaatcaa agaagatctt cggggcaggg tgaaattccc
    16981 taccggcggt atagcccgcg aactacttag gtggttgatt tggtgagatt ccaaagccga
    17041 cagtaaagtc tggatgagag aagatattta tggaagataa ataagtttat tttagtttgt
    17101 ttaaactata tccataaaaa ctcgaagata gggtcttcga gttttttgtt tttcctaaaa
    17161 aatataaaca tattgattag ggggaaaata attatgcaaa acacagcaaa aagaggcaat
    17221 atgatttcaa caagaacttt agtagtaatg tcaattttat ctgcaatatc ttatttatta
    17281 atgtttattc aagcaccaat accaggtata tttccagatt tcttaaaaat tgacttatca
    17341 gatattccag caatattcgg aggaatgtct ctaggaccat tagttggatt tggaatagtt
    17401 gtagtaaaaa atgtgttaca agcaataaca gcaacaacaa ctggaggaat aggagaattt
    17461 gcgaatatat taattggtgg ttcttatgta atgatacttt gttattcata taagagaagt
    17521 ggagatttaa aaggtgtact gattggtttt gtattaggaa caatatctat gactataatt
    17581 ggttcattag taaattattt tgtaatgttg ccactttatg gtcaacttat gggactagat
    17641 gctataatag gtttaggttc agctataaat cctcaagttc atgatttatt cacttttgta
    17701 atttggatga tagctccatt taatgtgctg aaggctgtta taatatcttt agtaacattg
    17761 ccactttata aaaaaatggg aaatataata aagaaataat tagacaagaa aatataaaat
    17821 taaatgtatt gaatagccat aatttattta taacttcagt cctatattgt gattgatata
    17881 ttaaatatat tatggcttta ttaatttatc aaaaaatgta tataaaataa gaaatattta
    17941 gacaaattta aattttaaaa taaaggaaga attaaaacga ttaactatta aaatatgaaa
    18001 aaggtatcta caatatacag agcgaattta taataaacaa aaaataaagt tattattaaa
    18061 aaaccataaa gattagtaat caaattttta ataataacta atttaataat gatgggagtg
    18121 tatattcatg aacttatcta aaaagataaa aaaattattt ttagatgata aagggtatta
    18181 ttttaatagt aattggaaag atattcatat tggtatttat aatacaaaat taggagattc
    18241 tttaaagtat atcactaatg aatatattga aaatctggaa agggtaaaat atgactatcg
    18301 tgaattaaaa aatatagaaa atcaaactga tggtatgtgt attttagcaa taaggcagaa
    18361 ttacaaggca ttagagtatg taaaaaatca aaccgaagaa ttatgtttag aagcaataaa
    18421 gtataattat aaagcacttg aatatgtaaa agagcaaaca gaatatatat gtcttgaggc
    18481 tataaaaaag gattgtaatg cacttaagta tgtacgaaat aaaacagaag ggcttattat
    18541 aaaagcaata agtcattctt ccaatataga tgttgtaagt atactaaagg ctttagagac
    18601 tcaaacaaga aggatatgtt tagaagcaat taaaaaagat ggcaggtgtc ttgcatacgt
    18661 tagagaacag agtgaagaat tatgtataga ggccattaag caaaattata aagcattaaa
    18721 atatgtaaaa aatcaaacgg aaaaaatgtg tatagaatcc gtaagacaaa atggtatggc
    18781 attacaatat gtaaataaac aaactgataa aatatgtata gaagctgtca aacaagatgg
    18841 aagaagtcta caatttgtca acaataagac agaagaaata tgtataaatg ctataagata
    18901 tttaaataaa aaatataata ttaaagatgt actaagttac atagataagt atacagaaga
    18961 tatatgtata gagattgtaa gacaaaatgg aaaaatgctt atgtatataa aaaatcaaac
    19021 agaaaaaatg tgtattgaag cagtaaaaga gaactataag tctcttaagt atgtaaaaga
    19081 gcaatctgag agaatatgta aagaagcatt gaagcaaaac cataaagcaa aagagtatgt
    19141 aaaaattgct attgatgatt gtatataagg tgtagataca gccatcaata gtgaaatata
    19201 aaaaaagata aagacaagta ctttataata atctaggctt atactataat taattaagca
    19261 gtgatataaa ttcacatgac gattatatca aaatggagat gaattgatgg caaaaatata
    19321 tttagaaaat gaaaataaaa ctagagaaat aggatataaa ttaggtaaac ttttaaaaga
    19381 aggcagtgtt atctgtttag taggagattt aggagctggt aaaactacaa tgacacaaag
    19441 cttggcagat tcattaggga tagaagatta tataacaagt cctactttta ctataataaa
    19501 tgaatatgaa ggaaaaatac ctttgtatca ttttgatgtt tatagaatag gtagtagtga
    19561 cgaaatgtat gatattggtt atgatgaata tgtaaattca aacggaatct gtataataga
    19621 atgggctaat ctaatagaag atatacttcc aaaagaatat ttgaatatag aattgagata
    19681 taaagatgaa ggaagagaaa tgatattaac tccaaaagga gagttttata aagagatagt
    19741 tgaggagttg attaaataat gaaaatacta ggtatggata cgtcttctat ggcagcaagt
    19801 gtagcagttg ttgaagatga taacttaata tgtgagttta cagtaaacaa caaaaaaact
    19861 cattcacaaa agcttatgcc aatgatagaa aatatgttga gtatgagtga tttaagtata
    19921 aaagatatgg atttattagc tgtatgtata ggtccaggtt cgtttacagg acttagaata
    19981 ggtatggcta cagtaaaagc tatggcccat gtaaataata taccaataat agcagtaaat
    20041 tctttggaat ctctagcaaa taacataaat ttctgtgata gaaagatatg ttgtatatta
    20101 gatgcgcaga aaaatcaagt ttattcagct aaatacaagt ttgaaaatgg agagatgata
    20161 gaattagatg gtgttgatgt tgtagagttt gatgctttag tagatgaaat tgtatcaact
    20221 aatgaagaat ttataatagt tggagaagct gtatacaaat ataaagacaa gttagagaat
    20281 ataaaaaata taaaaatacc ttcaccagct aataatgtat ctaacgcagg aagcttatgc
    20341 tctttagcgt taaataaata tagtagaaat atagatgtac atacttgtta tacgataaat
    20401 cctatgtata tcagaaaatc tcaagctgaa gtacaatatg atgaaaaaat gaagaggcta
    20461 aataatggaa gataatacaa aactaatagt agatgatata aaaatagaag aaatgactac
    20521 agaagatata gatgaagttt ttgaagtaga aaaaaattgt tttgaagatt attggtcaaa
    20581 agagtctttt agaaaagagt tgtctaatga agtagcaagg tatatagtag ctaagttaaa
    20641 tggaaaaatt gttggatatg tgggaatttg gcttatactt gatgaaggtc atataaataa
    20701 tgtggctgtt catagtgact atagagggaa aaaaataggg gacaagttga taaaaggcat
    20761 agttgattta tgtaaagata ataatattgc atctatgaca ttagaagtta gagcatcaaa
    20821 taaaatagca caaaatttat atagaaaata tggatttaaa atgggtggaa taagaaaaga
    20881 atactacagt gataataaag aagatgcaat tataatgtgg aaccaattaa aagaggtgta
    20941 gagtttatga gtgatataat aacattagct atagagagta gttgtgacga aacagctgca
    21001 tcagttttaa aaaatggtag agaagtactt tcaaacataa tctctactca aatcgagaca
    21061 cataaaaaat ttggtggtgt agttccagaa gtggcctcaa gaaaacatgt ggaaaatatt
    21121 gatatagtta ttcaagaagc tttagacaaa gcaaatatag gttttaatga tatagaccat
    21181 atcgcagtta catatggacc tgggctagta ggtgctttat tggttggtct atcttatgct
    21241 aaagcacttg catatacttt gaatattcca cttgtaggag taaatcatat agaagggcat
    21301 ctaagtgcaa actatataga gcataaggat ttaaaacctc cttttataac attaatagta
    21361 tctggaggtc atactcactt ggttgaagta aaagattatg gcaagtatga aattttagga
    21421 aaaacaagag atgatgcatc tggagaagca tttgataaga tttcaagagc tatgaattta
    21481 ggctatccag gaggtcctat tattgataag ctggccaaga atggaaataa acatgctata
    21541 gaatttccaa gagcctattt agaagaagat agctatgatt ttagttttag tggattaaaa
    21601 tcttctgtat taaattattt aaatggaaaa agaatgaaga atgaagaaat agtagttgaa
    21661 gatgtagcag catctttcca agaggctgta gtagaggttt tatctactaa agcactaaaa
    21721 gcagtaaaag acaaaggata caatatcata actctttctg gtggggttgc ttctaattcg
    21781 gggcttagag ctaaaataac agagcttgct aaagacaatg gtattactgt taagtatcct
    21841 cctctaattt tatgcacaga taatgcagct atgatagggt gtgcaggata ttataatttt
    21901 ataaacggga aaacgcatga tatgagttta aatgcagttc caaatttaaa gataaatcag
    21961 tagagttaga tgaatttgta aatataaaag tattgtttca aaaaagtaag tagaagtgaa
    22021 attaaatata ataaaaataa atatagtata tattaaaact tgaagtaaaa ctatttatga
    22081 tctttatatt ccaatgaatt gcaacaaatt ttaggatata cagattgtct ttggcttaga
    22141 tttgcttcaa gtttaatttt ttctatagaa atattatttt aaaaagtggg taatataccc
    22201 ttttttttca ttcttaaaaa gttttatact aattatagaa agattaagga ggggaagaaa
    22261 tgagtcaaag taaagaagac aaaataagaa gtattttgga agcaaaaaat attaaatcaa
    22321 attttcaaaa taaagaaaat ttaagtgaat ttaatgaaaa aaaagcaagt aaaagagcag
    22381 aagacttatt agatgtatat tataatacat tatctactgc tgatatggaa tttccatatt
    22441 ggtataatag agaatataga aaatcagatg gtgatatacc agtagttaga agagctaaag
    22501 ctcttaaagc tgctttttca catatgacac caaatataat acctggtgag aaaatagtaa
    22561 tgcaaaagac acgccattat agaggttctt ttccaatgcc atgggtaagt gaaagtttct
    22621 ttgttgctca aggagaacaa atgagagaag aggcaaagaa attagccagt aacacagctg
    22681 atgaacttac aaaatttggg tctggtggag gaaatgttac tgaaagtttt ggaaatgtcg
    22741 tttcaatagc tggtaaattc ggaatgagaa aagaagaagt acctgtactt gtaaaaatgg
    22801 caaaggaatg ggttggaaag tctgttgaag atttaggttt tcactatgaa aaaatgatgc
    22861 cagattatga tttaaaagaa aacctaatga gtacacttat atgcatgttt gattcagggt
    22921 atacacttcc tcaaggtaga gaagttataa attattttta tcctttaaat tatggattag
    22981 atggaattat aaaaatggca aaagaatgta aaaaggcagt tgcaggaaat gcatctggag
    23041 atgggcttat tggtatggac cgtctatatt tttatgaggc tgttattcaa gttattgaag
    23101 gattacaaac ttggatttta aattatgcaa aacatgcaaa atatttagaa agtatagaga
    23161 cagatttaga agctaaaaaa gaatactcag atttagtgga gatattagaa catattgcac
    23221 ataaacaacc tcgtacattt agggaagctc ttcagttgac ttatacaatt catatagcat
    23281 cagtaaatga ggatgctata tctggtatgt ctataggacg ttttggtcaa attttatatc
    23341 cttggtatga acaagatata gaaaaaggac taattacaaa agaagaggtt atcgaattat
    23401 tagagcttta tagaataaaa atcacttgta ttgactgttt tgcttcagcg ggcgtaaatg
    23461 gaggagtact atcaggcaat acatttaata ctttatctat aggtggatta aaagaagatg
    23521 gttctacagg agctaatgaa ctagaagagt tattattaga agcaagtatg agatgtagaa
    23581 ctcctcaacc tagtttaact atgctatatg atgaaaagct tccagaagat ttcttaatga
    23641 aagctgctga atgtacaaag cttggttctg gttatccagc atgggtaaac aactcaaatg
    23701 gaacaacatt tatgatgaaa caatttgctg atgaaggtat gactgttgaa gaagcaagag
    23761 catttgcatt aggtggatgt ttggaaacat ctccagggtg ttggaaacag ttaactttaa
    23821 acggaaaaac atactctatt gcaggtggag caggtcaatc tgctggctct ggagttcact
    23881 ttatagctaa tccaaaaatt ttagaacttg ttcttatgaa tggtaaagac tatcgtatga
    23941 atatacaagt atttgagcct cataataagc cattagatac ttatgaagaa gtcatagaag
    24001 tatttaaaga ttattataaa caagcaataa atgttttaga aagagcaaat aacattgagt
    24061 tagatatatg gagaaaattt gatacttcta taataaattc actactaaag ccagactgtt
    24121 tagacaaagg tcaacatata ggaaatatgg gatatcgtta taatgcaaca ttaaacgtag
    24181 aaacttgtgg tactgttact atggtcaatt catttgcagc tcttaagaaa cttgtatatg
    24241 atgacaaagc atttacaata gaggaaatga aggatgccat attaaataat tttggattta
    24301 aagatgcatt agaagtagga aattattcta tggcagacca agtaaaagta gataagactg
    24361 gtaaatatga tgctatttat aaggcctgtc ttgatgcacc taaatatgga aataatgatt
    24421 tgtatgcaga taatattctt aaaaactatg aggtatggtt atcaaaagta tgtgaagaag
    24481 cacaatcttt atatgctaag aagatgtacc cttgccaaat atctgtttct acacatgggc
    24541 cacagggagc tgctaccttg gctactccag atggtagact aagtggaaca acatattcag
    24601 atggttcagt ctctgcatat gcaggaacag ataaaaatgg agtatatgca ttatttgaat
    24661 ctgcaactat ttgggaccaa gcagtagttc aaaattctca gatgaattta aaacttcatc
    24721 caactactat aaaaggacaa caaggaacta aaaaattatt agatttaaca agaagttatt
    24781 taagaaaagg tgggttccat attcaatata atgttgtaga ttcagaaaca ttaaaagatg
    24841 cacaaaaaaa tcctgacaat tatcgtcaat taatggtacg tgttgctggg tttactcaat
    24901 attggtgtga attaggtaaa ccaatacaag atgaagtaat tgctagaaca gagtatgaag
    24961 gggtgtaaaa tatgagaaag catagtgatt gtatgaattt ttgtgcagta gatgcaacca
    25021 aaggaatttg tagattatca aaacaaatga ttaatttaga tgatgcagca tgtccagaga
    25081 taaaagtaat gccaaaatgt aaaaattgta aaaattttgt tgaagctaat gatgaaggta
    25141 taggtaaatg tgttggccta gagaaagaag actgggtata ttcaacattg aatgcaatta
    25201 cttgtgaagg acatgtgttt aatgagtagt caaaagcaat tagaaggcat gatttttgat
    25261 gtacaaagct tttcagttca tgatggtcca ggttgtagaa caactgtatt tttaaatgga
    25321 tgtccactga gttgtaaatg gtgtgcaaat ccagaaagtt ggactgtaag accacatatg
    25381 atgtttagtg aattatcttg tcaatatgga aatggatgta ctgtatgtca tggtaagtgt
    25441 aaaaatggtg cattgagctt taatcttgat aataagccag ttatagattg gaacatctgt
    25501 aaggactgtg aaagttttga atgcgtcaac tcatgttatt ataatgcgtt taaattatgt
    25561 gcaaaacctt acactgtaga tgaacttgtg caggtaatta aacgtgattc taacaattgg
    25621 agaagtaatg gaggagtaac atttagtgga ggtgagccac tacttcagca tgaattttta
    25681 catgaagtat tattaaagtg tcatgaggta aatatacaca ctgccattga aacgagtgca
    25741 tgtgtatcaa atgaagtttt taataaaata tttaacgata ttgattttgc atttattgat
    25801 ataaagcata tggatagaga aaaacacaaa gaacaaacag gtgtgtataa tgatttaata
    25861 ttagaaaata tatcaaacct tgcaaattct gattggaatg gaagattagt acttagagta
    25921 ccagttatct ctgggtttaa tgatagtgat gaaaatataa gtgatattat atcttttatg
    25981 cacaaaaata atttggtaga aattaatcta ttaccatttc acagattagg agaatcaaaa
    26041 tggactcaat taggcaagga atatgagtat tctgataagg gtgatgttga tgaaggtcat
    26101 cttgaagaat tacaagatat atttttggat aatggtatag cttgttatgt tggtcatgag
    26161 acagctttct aaaattaaat acaagtttta attaaaaaaa ggatgttaca ctaaaagcta
    26221 gagttacatc cttttttatg tggtttttat gtagaaataa ttaagtaaag ttttatatgt
    26281 tttatatatt gtttatattg ctttatgtcc ataaatattg ctttaaatat actttatata
    26341 ttttttatag tttaaattac agttagagaa tatattacgt ttacttcatt gaagcagaag
    26401 ttttgtggta aaatactact atgaaaaaat ttaatagaat atattttaga aacaactaaa
    26461 aataatagtt aggaggaaga catctatatg aacaaatcat ggagcaactt gattattaag
    26521 aataaagaca aaaaagtacg ttcagggtgg attattatag ctgttatggc taccttttac
    26581 atacttcagt attatttaag catgatactt gtggaaataa taagaaaaat cctggttgct
    26641 acaggtgaca ttaatttaaa aacaaactat ttttcagaat atgctaattg gttaaatgat
    26701 gttgggttac agattgtatt tcaaattcta acagattcac ttatgattat tattcctata
    26761 attgtatgga gatttattat gaaaaatcct attagtgaaa tggggttaca ttcttataga
    26821 attaagaaaa aagaaggata tgtaggaatg ttattaggaa tatttaattg tacagtaatc
    26881 tttattataa caataacaat tggtggtggt catgttactt catgggtacc aaaaatttca
    26941 cctttaacag tttcttggat acttgtcttt attttagttg cttttgcaga agaaatttta
    27001 aatcgtggtt tttttatggc agttttacga agatgtaaaa atatttattt tataatgatt
    27061 gttccatctg ttatttttgg gttgattcat atatggaatc ctgatgttac ttttttgtct
    27121 gtaattaata ttataattat tgggattctt ttttcatata tgtttataaa gtcgtcgaat
    27181 atatggatgt gtataggtta tcattttaca tggaatgtat ttcaggggat tatatatggt
    27241 atgcctgtaa gtggacttca ggttccaggg attataacta cacaaatcac tcatggtaat
    27301 ctattaaatg gagggatgtt tggtattgaa ggtggaattt tgactacttt tgttaatcta
    27361 cttagcttta tatttgtatg gtattactat agagattcta agtatgattt tgttagtgat
    27421 aaagtaaatg tgaccaatat caagaattag ttgattataa taaattttaa taatatcttc
    27481 taagcttaaa gtattctgta ggattgttgg aaatttgcaa aaatataaaa ataacttgga
    27541 tattctattt actccaagtt atttttatat tcttttgtaa atagaaatgg aacataaaaa
    27601 attaaaacaa ccatagatac tattagtata tcatggtaag tgatagttga ttctttagaa
    27661 tttgtgataa gaaaagcact attatttaaa acatgaatta tagcacaaaa ccagatattc
    27721 ttagttttta tataaagata tcctaaaaat attgaaattc caactatatg tattgaacgt
    27781 aatattattc ctaatattgg cgcttcaggt gtatataatg taaatattag tggtaagtgc
    27841 cacaagctcc aacacatacc taatattata actcctaact ttttaccaaa tttatcaaaa
    27901 aaaatattct gtaaaaaacc tctccaggca tattcttcac taaaaaaaat ataaatagta
    27961 gttaatgatg ttattggagt aaatataaat tttatgatac cactataatt aggttgagcc
    28021 ccttgtagta ttagtataaa tgatgttgga acaattacag aaatcataaa gtaaataatt
    28081 aataaagaac ctagtttaat attttttaca ggatataaat tttttttatt tgatgaatat
    28141 attataattg ataagctact tataaaagtt aatattaggt ttataattat aaattcatta
    28201 ttatttatta gatttaataa cttaagaaca attaataatg aataaataag aaaaaataat
    28261 atgattag
//
LOCUS       DAFKMX010000031        27385 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFKMX010000031 DAFKMX010000000
VERSION     DAFKMX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.031"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..507
                     /locus_tag="LZB08_001109"
     CDS             <1..507
                     /locus_tag="LZB08_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7314622.1"
                     /translation="YLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVAGLQEHIANQR
                     NDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWYG
                     RKIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGL
                     RLITIQNK"
     gene            625..930
                     /locus_tag="LZB08_001110"
                     /pseudo
     CDS             625..930
                     /locus_tag="LZB08_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            943..1848
                     /gene="truB"
                     /locus_tag="LZB08_001111"
     CDS             943..1848
                     /gene="truB"
                     /locus_tag="LZB08_001111"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBM7314623.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1979..2908
                     /locus_tag="LZB08_001112"
     CDS             1979..2908
                     /locus_tag="LZB08_001112"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBM7314624.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3012..3269
                     /gene="rpsO"
                     /locus_tag="LZB08_001113"
     CDS             3012..3269
                     /gene="rpsO"
                     /locus_tag="LZB08_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBM7314625.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3509..4354
                     /locus_tag="LZB08_001114"
     CDS             3509..4354
                     /locus_tag="LZB08_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBM7314626.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4479..6590
                     /gene="pnp"
                     /locus_tag="LZB08_001115"
     CDS             4479..6590
                     /gene="pnp"
                     /locus_tag="LZB08_001115"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBM7314627.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6872..7615
                     /locus_tag="LZB08_001116"
     CDS             6872..7615
                     /locus_tag="LZB08_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7314628.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7773..9020
                     /locus_tag="LZB08_001117"
     CDS             7773..9020
                     /locus_tag="LZB08_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBM7314629.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9055..9300
                     /locus_tag="LZB08_001118"
     CDS             9055..9300
                     /locus_tag="LZB08_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBM7314630.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9310..10533
                     /gene="dapG"
                     /locus_tag="LZB08_001119"
     CDS             9310..10533
                     /gene="dapG"
                     /locus_tag="LZB08_001119"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBM7314631.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10646..11380
                     /locus_tag="LZB08_001120"
     CDS             10646..11380
                     /locus_tag="LZB08_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBM7314632.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11503..13914
                     /locus_tag="LZB08_001121"
     CDS             11503..13914
                     /locus_tag="LZB08_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBM7314633.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13917..14975
                     /gene="mnmH"
                     /locus_tag="LZB08_001122"
     CDS             13917..14975
                     /gene="mnmH"
                     /locus_tag="LZB08_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBM7314634.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14969..16303
                     /gene="rimO"
                     /locus_tag="LZB08_001123"
     CDS             14969..16303
                     /gene="rimO"
                     /locus_tag="LZB08_001123"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBM7314635.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16290..16832
                     /gene="pgsA"
                     /locus_tag="LZB08_001124"
     CDS             16290..16832
                     /gene="pgsA"
                     /locus_tag="LZB08_001124"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBM7314636.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17120..18166
                     /gene="recA"
                     /locus_tag="LZB08_001125"
     CDS             17120..18166
                     /gene="recA"
                     /locus_tag="LZB08_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBM7314637.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18342..19883
                     /gene="rny"
                     /locus_tag="LZB08_001126"
     CDS             18342..19883
                     /gene="rny"
                     /locus_tag="LZB08_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBM7314638.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            20197..22533
                     /locus_tag="LZB08_001127"
     CDS             20197..22533
                     /locus_tag="LZB08_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7314639.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            22915..24696
                     /locus_tag="LZB08_001128"
     CDS             22915..24696
                     /locus_tag="LZB08_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBM7314640.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRVLFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(24778..25308)
                     /gene="lepB"
                     /locus_tag="LZB08_001129"
     CDS             complement(24778..25308)
                     /gene="lepB"
                     /locus_tag="LZB08_001129"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBM7314641.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(25348..26172)
                     /locus_tag="LZB08_001130"
     CDS             complement(25348..26172)
                     /locus_tag="LZB08_001130"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBM7314642.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            26429..27337
                     /locus_tag="LZB08_001131"
     CDS             26429..27337
                     /locus_tag="LZB08_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7314643.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
BASE COUNT        11110 a         2893 c         4946 g         8436 t
ORIGIN      
        1 tatcttaaga agtcattaaa caagcttgct aaattacaaa gagaattatc gagaaaaaca
       61 atcggtagtt taaatagaaa taaagcaaga ttaaaagttg caggacttca agaacatata
      121 gctaatcaaa gaaatgattt tctacaaaaa ctatctacta aattaataaa agagaatgat
      181 attatttgca tagaagattt acaagtaaag aatatgatta gaaatcgtaa actatctcgc
      241 ttaatttcag atgtatcatg gtcagaattt attcgtcaat tagaatacaa ggctaattgg
      301 tatggtagaa aaattgtaaa ggtaggtaaa ttctttgcaa gttcacaaat atgcaataaa
      361 tgtgggtata aaaacgagga agttaaggat ttaaatataa gagaatggat ttgtcctagt
      421 tgtaatgaaa ctcacgatag agatataaat gcaagtataa atatattaaa agaaggatta
      481 agactaataa cgattcaaaa taaataactt atgtaagaac cgtaggaact acggggatag
      541 cctgtggaga attagtaaga catattttaa tatgcaaaat tctgtgaagc aggaaccctg
      601 tgactttagt catgggaggt tcagtcaaag gtatctagta tagttttaac tcaagacatg
      661 atgaatagaa ataatattag ctttaatgat gttgatggaa taacaacata tacaagagac
      721 ataaaaggta tagaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagtg
      781 agttttaggt caaaaaatta tgttgatgta agtgaaatag ccaagttatt tggtggtggt
      841 ggtcatgtaa gggctgctgg atgtactatt agagatagta ttgataatgc aaagaaaatg
      901 gtactagagg cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt
      961 atattgaaac ccacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta
     1021 aatataaaaa aagttggtca tacaggtact cttgacccag atgcttcagg tgttttacca
     1081 atctgtatag ggaaagctac aaaagtatgt gaagttattt taaacaaaga taaatcgtat
     1141 atatgtgagc ttactttagg tataagtaca gatacttatg atgcttcagg tgaaatttta
     1201 aaaaaagttg atgattttaa atttagtaat gaagacatag aaagagcttt tgacacacaa
     1261 agaggagaga taaatcaact tcctccaatt tattcagctt taaaggtaaa tggaaagaga
     1321 atgtgtgatt tagtaagaag tggtagacaa tctgaaatta ctttaaaaac aagaagagta
     1381 aatataaaag acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag
     1441 tgttctaaag ggacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt
     1501 ggagctcata tgtcgttttt gaatagaaca tcttctggta aatttgactt agataattca
     1561 ataacattag aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat
     1621 gatatagatt atgtccttga ttcgtttaat tatgttgtat taaaccctaa tgctataaaa
     1681 tattatagta atggtggctc aatagatgat aaaaggtttt taaaaaataa tttcgataaa
     1741 gatgatgaat ttgtaagagt atacagtaca gataatttct taggattagg aaaattatca
     1801 aaacacaata atacaataag tgttaaaagt gataagatgt tcatataacc aatgtattta
     1861 aaaaaacaac aaaagagata gtactataat aacttctata gtctatctct tttacttatg
     1921 ctataatagt ttgtgaataa gttaagagta aattatattt tagcggggaa catttattat
     1981 ggtgattata aaatctataa aagaaatagc aaacataaaa gaaagtgtca ttaccatagg
     2041 aaattttgat ggtgtacaca aaggacatca agttcttata ggaaaaacag ttgaacatgc
     2101 aaaaaaagaa aatatacaga gtatcgtatt tacatttgca aatcatcctg taaattactt
     2161 tagaccaaac tcaacaaaaa atattataag caatgatgat aaaataaaat tattagaaga
     2221 tttgggaata gatatagttg taatcatacc atttgatgag tatatgacta aaatacctgc
     2281 aaaggatttc gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga tagtaattgg
     2341 acatgacttt acatttgcaa gggcaaaaga aggcaatgtt aatctattaa aaagcttaga
     2401 acatgaattt ggttttgagg tagaagtaat aaaacccatc aaaatcaatg atgtaagagt
     2461 gagtagtact tatattagaa gtttagtatc ccagggtgat atggctaatg tcaaggaata
     2521 tttgggtaga aattacaagt tagaaggttg tgttatacat tctaaacacc ttggaagaac
     2581 cataggattc ccaacggcaa atattgacct taagaataat atgctcgttc caaagagagg
     2641 tatttatgct tctattgtac atattggaga tgagacttat tttggtgcta caaatgtagg
     2701 ctataaccca acagttaatg gaaaaacttt gtctattgaa acaaatattt tagagtttga
     2761 tagagatatt tatggggaaa atataattgt tgaatttttg gaaagaataa gagatgaaag
     2821 aaagtttaat tcaatagatg acttaaaaaa tcaattgtat aatgatacaa attatgtgta
     2881 tgaaaactat gtttgcaaaa acaagtaatg tatgataaaa tgattaagga aaataaccat
     2941 tactcggcat ttgaatactc caatcaatgc ttagtaatag gcgtttctaa aaaaatacgg
     3001 aggtaatcta aatgataaac aaatcagaaa taataaaaga atatggaaga gctgaagggg
     3061 atactggttc tccagaagta caaatagctt tattaacagc tagaataaac gagttaaatg
     3121 gacatttaaa aactcataag aaagaccacc attcaagaag aggactatta aaaatggttg
     3181 gtagaagaag aaacttatta gcttacttaa aggaaaaaga tcttgaagga tatagagcat
     3241 taatagctaa attaggatta agaaagtaat ttcattcttt ataaaaaagc aggtatagtg
     3301 tatacctgct tttttatagt taattcatta taaagtaaaa tagttaataa taaaagtagt
     3361 ttatatgtta taaaaaatta taaaatgaat aatattgggt aagaatatta aaatagtata
     3421 caaatacata atcaatttaa aaggaaagac aggataaata tagaaatttt ataacaggat
     3481 aattagttgg aataaggagg atattatttt gaaaataaag ttagtatgcg atagtttgtg
     3541 cgatattcca gatgaaatat cagaaaaaga ttatatagag atagttccac ttacagttat
     3601 ttttaatgat agggaatata tagaaggagt agatataaag aaagaagagt tttataaaaa
     3661 agtaaaagaa atcaagcaaa tacctaaaac ttcacaagct acatatatgg agtttaaaga
     3721 agtgtttgat aagttcataa cagaaggtta tcatataatc tgtatgacag gggctgcaaa
     3781 cgcatcagga acttttcaaa gtgctatgat agctaaaaat gatgtaaatg aaaatgaaaa
     3841 aatacatata tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc
     3901 atgtgaactt cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac
     3961 tcgcagtagt gtaagattgc tttttgcacc atatacactt gattttttaa agcagagtgg
     4021 aagagttcca gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt
     4081 ctttgataat ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag
     4141 taaattggtc gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac
     4201 aataggttgt ggagataatt tacacgattg tgaaatatta aaagatgaag taaataaaag
     4261 aataaaagca agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg
     4321 gccagatata ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat
     4381 atgtgaagat aattttatat tgttaattta tagtattttt gataatatag taaatagagt
     4441 gatatacgtg ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta
     4501 aaatggattt tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcta
     4561 gtggtagttg tatagttcga tacagtgata gtatggtaat ggtaaataca actaaatcag
     4621 ccaaacctag agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat
     4681 actctgtagg taagatacct ggaggttttt taaagagaga aggcaagcct tcagagaaag
     4741 ctatacttac atcaagattg atagatagac caataagacc tttattccca aaaggattta
     4801 gaaatgatgt tcaggtagtt gcaacagttc tatcagtaga ccaagattgt acaccagata
     4861 tagtagcaat gataggttct tcaatagccc tttctatatc agacatacca tttaatggac
     4921 caacaggttc agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag
     4981 agcaaagaga aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga
     5041 tggttgaagc cggagctgat gaagttccag acgaggtaat gcttgatgct attttatttg
     5101 ctcaccaaga aataaaaaaa atagttgagt ttatagaagg tatagttgca gaagttggaa
     5161 aagaaaaaat gcctgttgaa ttataccatg caggagaaga aataactcaa ttggttcgcg
     5221 aatttgcaac tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg
     5281 aaaatatgga tagagttaaa gaagaaactt tagctcactt tgaagaaact ttagaagatt
     5341 ttgaagattt tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa
     5401 gaaaacttat agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac
     5461 ctatatggtg tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag
     5521 gacaaactca agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaactag
     5581 atgggttaga tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata
     5641 gtgttggaga agctagacca tcaagaggac caggaagaag agaaatagga catggtgctt
     5701 tagcagaaag agctttatta ccagtaatac catctcaaga agaatttcct tatgctatta
     5761 gattagtttc tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggct
     5821 caacattatc tttacttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa
     5881 tgggacttat aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag
     5941 accatttagg cgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac
     6001 aaatggatat aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag
     6061 ctaaagaggg tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac
     6121 cagaactttc accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa
     6181 aagatgtaat aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta
     6241 agatagatat tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta
     6301 agaaagctca agaacttata aataatatag tagttgaacc agaagtagga aaaacttata
     6361 aaggtaaggt aagtagaata atgaactttg gagcatttgt tgagatactt ccaggaaaag
     6421 aaggattact tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat
     6481 tgaatatagg ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta
     6541 atttatcaag aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat
     6601 aaataactta ttatataatt aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa
     6661 aagtagataa ttttaaaatg atagttatgg atggcttata gttctttttc tgtcaggctt
     6721 tactcttata aagaagctat ttaaaagctg attaatagag agctttattg atatatatag
     6781 aatcacttaa gttatattta gtgcataaac agagcttatt tttaatagta ttaaataaga
     6841 aacaatttgt acataattag ggggagtttt aatgataatg atggtactta ataagagtaa
     6901 gctcaaaaaa atcataatac taattttagt agtgttatta gtattatcag gaggggtaat
     6961 ggtattaaag ttatttaaca acaagccagt atttaattta tttaatggtg tagatttacc
     7021 atatgaaaga ggtacaaagg ataaaagtgg atatgttgca ttaacctgta atatagacct
     7081 tggatgggaa acagagtacg tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa
     7141 gattacattt aatgtaactg gaaaatgggc agaaaagaat aaggatgaat tattaaagat
     7201 aaaaaaacaa ggacatgaaa taggtaatca tggatataaa catttagatt attctacatt
     7261 atcatatgaa gacaattatg agcaaataga gacctctaaa aagataattg aagaaattat
     7321 aggtgaaaaa actaaatttt ttcaagcacc agcaggttct tttggtccag aaacagtaaa
     7381 ggctgctaaa gcattagggt atacttctat caaatgggat gccgatacta tagattggaa
     7441 atacaaagac caaccagagg taatcattga tagaatgaag aaaaaagaca ttaaagatag
     7501 tagtataatt ttaatgcatc caaccaacgc aacgacaaaa tgtatagatg atattatagc
     7561 tattgtaaga gaaaaaggac taaaaccagg aaaacttagt gatgtattta agtagacaaa
     7621 gtataaatat ttggttaaga ttgtataagt tgtagttgaa ttaattaaaa gactgactta
     7681 taatgtaata catataaaaa ataaatataa ttaaaacata gtgttttcaa taatccaata
     7741 tgaaacaaag aataaattgt taggagaatg taatgtataa gacaaaaata ctggaaaatg
     7801 ggcttacaat aattggtgaa gaaataccat atctaaagtc aataacatta ggtatttgga
     7861 taaatgcagg ctctaggata gaagaagcac aggtgagtgg aacttctcat tttatagagc
     7921 atatgatgtt taaaggaacc aaaaacagaa catcaaaaga gattgctagt tcaatagata
     7981 atttgggtgg tcaaataaat gcatttacaa gtaaagaatg tacatgttat tatgtaaaac
     8041 taatagatga gcatatagat acaggaattg atgtattgag tgatatgata ctaaattcta
     8101 aatttgataa aaatgatata gataaggaaa gacttataat attggaagaa cttaagatgt
     8161 atgaagactc tccagatgac ttatcttatg atttattagt ggaaaatata tatgctaatg
     8221 atggattagg aatgaatata attgggacaa aagaatctct gtacaatata actagagaat
     8281 ccatgttaga atacttaaat aagtattata ttccaaataa cgctgttatt tctatagctg
     8341 gaaactttaa ctttgatgat atggtggaga aaatcaaatc aaaatttgga cactgggaaa
     8401 agaaaaattt aagtatagac ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa
     8461 ataaagatac tgaacaggtg aacttagcta tgtgcctaaa aggtattcct tttgaaaatg
     8521 atgaagaagt ctattcaatg gcagtggtaa ataatatttt cggtggaagt ataagctcaa
     8581 ggctttttca gaaaataagg gaagaaaaag gtttagtata ttcaatatac tcatcacaga
     8641 cattatatag gaaatgtggt gaattaggta tatttgcaag catgagtaca gaaaatttac
     8701 aagatgtata taatttaata aagaaagaaa ttgaaaatat aagagaaaac tatcttacag
     8761 aaaaagaaat atctgaaagc aaggaacagt taaaaggaaa ttatatattg gatttagaaa
     8821 gtactagcag tagaatgatg tctactggta aatctatgtt acttagcaaa aaagtaaaaa
     8881 ctacagatga aatacttgaa tgtataaata atgttaatat taattcaata aagaaggttg
     8941 ttgataaggt attcaatatc gaaaatattg gtacatgtat agttggaaga gatgtagaaa
     9001 agattctaca cttggattaa tattgaaaaa agtaaatctc tggaggtata aaatatgaat
     9061 ttatctgaaa tagcaggtaa ggaaatagtt aacttggtca caggagaaag attgggggtc
     9121 attggagaat gtgaccttat actagatgaa actacaggcg gtatattagc gcttctaatt
     9181 ccaaaagaaa aaggattttt tgccagaaga aaagataagt catttttaga agtaccatgg
     9241 agaaatgtaa aaaaaatagg taatgacatg ataattatag aatatgaggg atatatttag
     9301 gaggaaaaaa tgagtatttt agtgcaaaag tttggtggga catcagtaga atcatatgaa
     9361 aaaatgaatg aagtatgtaa gatagtaaaa gcatacaaaa aaaatgatga agaattacaa
     9421 ttagtattag tagtttcagc aatgggaaga aaaggagctc catatgctac agatacactt
     9481 ataaatttgt gtagtgcagt aaatgatgag ccaagtaaaa gagaacttga tttaataatg
     9541 tcttgtggag aaataatatc aggaacaatt cttgctaatt tattgaatgc acaggggata
     9601 gattctgttt tctttactgg tcagcaagca ggtataatta caagtgatga atattctaat
     9661 gcaaagataa agtatataaa tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt
     9721 gtagttatag ctgggttcca gggtgtaaca gatgatggag aaataactac tcttggaaga
     9781 ggcggaagcg atacatcagc agttgctatt ggtaaagctt tagaatgtga aacagttgaa
     9841 atttacactg atgtggatgg tataatgact gcagacccta gagtagaacc aaacgctaaa
     9901 gtattgagtt ttatagacta tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta
     9961 atacatccaa gagcagtaca gttggctaaa agtggaaata taactttagc cataaagaat
    10021 acaatgaatc caacttttga aggtacaaaa ataggttctt tatgtagaca cttagaagac
    10081 aatatagaat atgaacaaga aagagatttt aaggttgctg ttgcaaataa agatagcgta
    10141 gcacaagtta agattaagtc agcagaagaa gtgtttacag aagtattaaa tgagatagaa
    10201 aagaaactta taactataga tatgataaat ttctttatat cagaaaaagc atttgttgta
    10261 gaagatgctg atataaaatc attaaaggaa atactagata aatttgaatt agattatgaa
    10321 gtaaatagag actgtgctaa ggtaacattg atatgttcaa gaattgctga tgaaatgtct
    10381 ggtatcatgt caaaggtagt tagaggatta tcaaaagcag gggtatctct tttacaaaca
    10441 tcagactcaa atatgactat atcttgttta gttagtgaag aagatatgca tacagcagta
    10501 catgctatac accaacaatt ttatttaaaa taaactgtat aatacaatta ttttttaatc
    10561 aaatttaaga atttcatata tatcttaata tatttgattt atttggtaaa actaaattaa
    10621 atataatttt gataggagtt tttttatgaa agattataga gaagataatg aaagatttga
    10681 taaagataat tgtagtgaag aatctagttg cgaagagaga gaatgtataa agcaatttgg
    10741 taccaatgaa atgccacctc aacctcctaa agatattcaa tgtataacta taattggaga
    10801 aattgaggga cattttatag gaaatcctca aaaaaaagct acaaagtacg agcatataat
    10861 tccaatgcta tactcaattg aggaaagtaa tgatgttaaa ggagtgttag ttgttttaaa
    10921 cactgttgga ggagatattg aagcaggttt agctattgca gaacttttaa acagtacatc
    10981 taaaaaagta gttactcttg ttctaggagg aagtcatagt ataggtgttc ctcttgctac
    11041 agcaggagat tactcattta tagcaccaac agcaaccatg attatacatc cagtgcgaac
    11101 tactggtctt gtgattggta taaatgagac ttttgagtat tttaaaaaga tgcaagatag
    11161 aataattcaa tttataataa gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat
    11221 gcatgaaaaa gatgaacttg taagtgatgt tggtagtgta ttaataggaa aagaagctgt
    11281 ggattatggt cttatagatg aagttggagg actaaaagaa gcacttaaaa aattaagaga
    11341 gcttataaaa gaatctgagg aagaaaaaaa taatgattaa aatttgttta aataataaaa
    11401 tttatgatat aatgatgtaa taaagtagga gtgttagtgt taaggcatta gcactttttg
    11461 ctgtgaaggg gtaaaacttg ctaaaaataa ggagtgatat taatggccac gaaaaagaag
    11521 aaaaaaaaga aggtaaatac cttaagtttt aatgcagaat atcataattt aataacaata
    11581 tttataggtg tatttttatt atatagttta aattctaatt ctatgggatg gatacctgtg
    11641 ttaatgcaaa atctttttaa gggactattt ggagggcttt caatagcaat accatttata
    11701 gtaattataa caggactttt aggatttttt gatggaaatg aatatatata taggctaaga
    11761 aagactaaac tatactacat aattatttta tttatatttg tattttatgg attattaaat
    11821 gcaggaactc ttcctgtaga tagcccacta aaaggtaaca tgtttgatga tgttatgaaa
    11881 ttgggtgtaa gtggacaagg tagtggtttg atagcaacaa ctattgcgta ttatatgagt
    11941 aaaatatttg gtatagcagg tggatggtta ataagtatct tcgcacttat actttctgta
    12001 atgtttatat ttaatatttc tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa
    12061 gcatctaaag acagcaattt aacgtttaaa gataaaattg caaatatgaa aaaatctgca
    12121 atagatatga tgactgatga agtagatgat actactataa ataaaaaacc aggtttcttt
    12181 aaagggttaa tgtcaaaagg tcttaataat gatgatgaag aagatgaata ttttgaagaa
    12241 gaaaatagtg atggtgtaga tgataaaact ataaaaatag ttggatttaa taaggcagaa
    12301 gatgagtatt tagaaatatt agaaggaact caaagtatgc cagagttaga tgttttaaaa
    12361 gaattacaaa aagctaccaa tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt
    12421 gatatagcta aacctaattt aaatatagaa aaaactcaac ctatgagtat tgtagcagag
    12481 ccagttaatg aggattattc aaactataaa aagcctagca ttgagctttt aaataaagta
    12541 aataagaagt ctgatgaaaa tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa
    12601 aagacactat cagattttgg agtggaagca aaaattaatc aagttacagt tggacctact
    12661 ataacaagat atgaaataca accaagtcca ggagttaagg taagtaaaat tgtaaattta
    12721 actgatgata tagctttaag tttagcagct aaaagtataa gaattgaagc cccaattcct
    12781 ggtaaaagtg caattggaat agaagttcca aatgaagaag ctcaaatggt tggtgtgaga
    12841 gaagttctag aaagtgaaga atttaataat tttaactctc ctcttgcaat gggacttgga
    12901 aaagacgtag caggaaaaat tataattgga gatataggta agatgccaca cttactaata
    12961 gctggttcta caggttcagg aaagagtgtg tgtgtaaata cattaataag tagtatttta
    13021 tataaggcaa atcctgatga agtaaaatta ctattaatag acccaaaagt agttgaactt
    13081 gcaaattata atggaatacc acatctattg ataccagtag ttactgaccc taaaaaagct
    13141 gcaaatgctt taaattgggc agttacagag atgaatagaa ggtacaaatt atttgcagat
    13201 gctcaagtaa aagatgtaac aagttataat gagaaagcgg aagaaaaact tcctaaaata
    13261 gtaataataa tagatgagtt agcagattta atgatggcaa gtgcaaatga tgtagaagat
    13321 tatatttgta gattagctca aatggcaaga gctgctggaa tgcacttgat tgtagcaact
    13381 caaagaccat ctgttgacgt tataacaggg gtaataaagg ctaatatacc atctagaata
    13441 gcatttgcag tatcatctca aactgattca agaacaatcc ttgatatggg tggtgcagag
    13501 aagcttctag gtaaaggtga tatgttattc tatccacttg gtgcagcaaa accagttaga
    13561 cttcaagggg cattcatatc agagagtgag tcggaaaaag taatcgattt tgtaaaaagt
    13621 caggttaaag atggaataaa atatgaagaa gatataattg aaactatatc taaggttaat
    13681 acttcaaagg gtagtgatga ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa
    13741 agtggtcaag catctgcctc tatgctacag agaagattta aaataggatt taatagagca
    13801 gctagactta tagattctat ggaagaaaga ggaataattg gagcaagtga gggaagtaaa
    13861 ccgagaaagg tattaataag caaacaggat ttgcagaatt tagaaggtga atagaattgt
    13921 cagttataaa aatagaaaaa gcacttaagc tagataaagt aatatttgta gatgtgagaa
    13981 ctgaaggaga atatgaagaa gaccatatat tgaatgcaat caatatgcct ctttttaaaa
    14041 ataatgaaca taatgaagtt ggaacaattt ataagatgca gggaaaacat gaagcaatac
    14101 aaaaagggtt tgactatgta tcgtataaat taaaggatat atatttacaa gcagcagaac
    14161 ttgcattaaa ttatgataat attgtcatat attgtgcaag aggcggaatg agaagtggca
    14221 gtatagtaaa tttattatca tcattaggtg taaatgtata tcaacttgaa ggtggatata
    14281 aggcttatag aaattttgtt ttagagtatt taaggcatgt aatggataca aaaaactttg
    14341 tatctgttca tggattgact ggtacaggaa agactgattt gttacattta ttagatgaaa
    14401 aaggaataga cgtgctggat ttagaaggca tagctaaaaa cagtggttct acctttggtt
    14461 ttataacttt tgataaaaag ccaccatctc aaaagaactt tgaaacaaaa ctatttgaaa
    14521 gtatattttt ttcgaaagag aattatatat ttgtcgagag tgaaagcaaa cgagttggaa
    14581 gtgttacagt tccaaatgaa attttcgagg caatgacacg agaaggacat catatattat
    14641 tagaatgtga tattgaaaat agagttgata gactttgtag agattatatt tatgataaag
    14701 atgaaggtaa tatattgatt ttaaaagaat gtataaacaa gtttagaaaa agattgggta
    14761 ataaagtagt agatgattat attgatttat tagaatcagg tgaatataaa gaacttgtaa
    14821 aaagatatct tttagaatac tacgacccac tttatatgca ttcagtagag aaatataagt
    14881 atagtaagat tataaatttt aatgatacac aaaaagcttt agaagatgtt atagacttat
    14941 atgagagtat attggaagga gaaagtgaat gttaaaaata gcattggaat cattaggatg
    15001 ctcaaaaaat ttagtagatg cagaaataat gatgggaatt ttaaataaca aaggctataa
    15061 attaattgga gattttgaag aagctgatgt aattatagta aatacatgtg ggtttataga
    15121 gtcagctaaa caagaatcta tagatacgat aataaacttt gcagagttaa aaaaaactgg
    15181 caatttaaag ttgttgatag taacagggtg tctagcccaa agatattctg aagaattaaa
    15241 aactgaaatt cctgaaatag atgctatagt aggaacagga agctatcaaa atatagataa
    15301 aatacttaaa gaattaagtg aaatgcatca aattgtaagc cttaatgata tagagtttgt
    15361 atttaatgaa gatttaccaa gatatatatc tacacctagc tatatggcct atttaaaaat
    15421 tggagaagga tgctccaata attgtactta ttgtataata ccaaaactaa gaggtaaata
    15481 tagaagtaga aaatttgaag atataattaa agaagctaag aaattagctg aatctggagt
    15541 aaaagaactt gtggttatag cacaggatac gacaaagtat ggttttgatt tatatggaaa
    15601 agaaagatta tcagaacttt tagaagaact tgcaaaaata gatggattta agtggataag
    15661 ggttatgtat tcatatccag aatctataac agaagaattg atacaagtta taaagaaata
    15721 cgataatata tgtagttact ttgatatgcc aatacagcat gcaagtaaca atattttaaa
    15781 attaatgaat agaaaaacta ctaaagaaga tatattaaat aaaatcaatc tgataagaag
    15841 taatatacca gatgctatac ttagaactac aataatagta gggtttcctg gtgaaacaga
    15901 agatgacttc aaacaacttg tggactttgt tgaagaggtt aaatttgata gacttggtgc
    15961 atttgcatat tcaagagaag aagatacacc agcagataga ttgccaaacc atatagatga
    16021 agaagtaaaa atacaaagaa gagatacttt aatgatgatt caacaaaaaa tatctgaaga
    16081 acttaatgac aaaaagatag gaaaaactta tgaggtgtta atagaagaac agatagaaga
    16141 taatgtatat acaggaagaa cacaaggtga tgcagaagaa attgacagta tagtgtatgt
    16201 aaaaagtgta gacaatcttg aagtaggaga atttgtaagt gttcaaataa atgatgcaat
    16261 ggaatacgat ttaatgggag atgttttata tgaacttgcc taacaagtta actttgttta
    16321 gaatattttt aataccagta tttgttttaa ttatgttgct caatgtacca aataaatttt
    16381 taatagcttg tattattttt ataatagctt caattactga tgctttagat ggaaaaatag
    16441 ctagaaaata caatctagtc actgactttg gaaaatttat ggacccatta gcagataagt
    16501 tattggttat ttcagcactt acatgtatga tagaagacca tcttgtaagt agttggatgg
    16561 taattataat agtggctaga gagcttacag ttagcatatt aagagctata gctgctgctg
    16621 atggaaaagt aattgctgct ggaaacagtg gaaaacttaa aacgataact caaatggtat
    16681 ctatagtgtt tttattgtta ggtgctcagt ttgaaaatgt attgatatta aatattggtg
    16741 aaattctcat tcttatagct acactactta cattatattc tggatgggaa tatctatata
    16801 aaaataaaga gctttttatg tcttctaagt aaaaaattat gctttaataa ataaaaacta
    16861 atgattaata aataaaaaat aacaacttaa aattttaaaa atgtaaaaaa gggtatacta
    16921 ttatagtata cctcttttca ttttaagaat attttctttt gtgtatataa ttattaataa
    16981 tggtttataa ataaaatgta atgaataaaa atataatcat tagtagcgtt tttaaattga
    17041 ctaggtttga tttatgttat ataattaata tataacaaga gaacaaatgt ttgtagataa
    17101 atgaagggat ggtatagaga tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc
    17161 tttaggtaaa attgaaaaag attttggtaa aggttcagta atgaaattgg gagaagcaac
    17221 atctatgtct atagatgtta tatcaacagg agcgattggt ttagacatag ctattggtat
    17281 aggaggtcta cctagaggga gaatagttga agtatatggt ccagaatctt ctggtaagac
    17341 tactgttgcg cttagttgtg tagcatcagc tcaaaaagat ggaggaatag ctgcatttat
    17401 agatgcagaa catgcacttg acccagtata tgcaaaagct ttgggtgtgg atgttgataa
    17461 cctaataata tctcaaccag atacaggtga acaggcttta gagatagcag aggcattgat
    17521 aagaagtgga gcgatagata taatagtaat agactcagta gcagcattag ttccaaaggc
    17581 tgaaatagat ggagatatgg gtgattctca tgtaggttta caagctagac ttatgtcaca
    17641 agcacttaga aagttaactg gttcaattaa aaaatcaaat tgtgttgcta tatttataaa
    17701 ccagttaaga gagaaagtag gaataatgtt tggaaaccca gaaactacta ctggaggacg
    17761 tgcactaaaa ttctattcat cagttagatt ggatgttaga aaaatagata caataaaaca
    17821 aggtgataaa gttataggta gtagaactag agttaaagtt gttaaaaaca aagtagcacc
    17881 accatttaag caagctgaat ttgatataat gtatggagaa gggatttcaa aaattggaga
    17941 ccttttagat atagctgctg atgtagatat agttaaaaaa tcaggttcat ggtatagtta
    18001 caatgatact aaacttggac aaggaagaga aaatgttaaa aaattcttgg aagataattt
    18061 agatttaact actgaaatag atgagaaagt tagagcattt tacaatttaa atgaagaaca
    18121 tgaagaatca ggtacttcag tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa
    18181 aaataactag taattaaaga catgccacgc tccctattag cttgacacaa tgttgaaaaa
    18241 attataaaat taagtgagga taaaatatct aaatttaatt ttaattatta ggaatatttt
    18301 ttaatcatag tgatgacatt ttgggattat taacgaagga ggtggatgtc atagatagtg
    18361 tagtaatgat tgtaataggt gcagctgttg gtattatagc tggatatttt gttagaaaaa
    18421 atatatctga agcaaaaatt ggacaagctg aaaacttggc caaagaaata atagataaag
    18481 cacaccacga ctcagaaaca gtacaaaaag aaaaattact agaagctaaa gaagagatcc
    18541 ataagtggag aacagaagct gaaagagaaa acagagaaag aagaacagaa gtacaaaagt
    18601 atgaaaaaag agtagtacaa aaagaagaag tcttagatag aaaattacaa aatttagaat
    18661 ctaaagaagt aaacctaagt gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg
    18721 aagtaataaa aactcaacag ttagaaaaat tagaaagtat atcaggaatt acttctgata
    18781 aggccaaaga aatcatctta accaatgcgg agagagatgt aagacgtgaa atgtctataa
    18841 tgataaaaga gatagaaagt caagctaaag aagaagctga taagaaatca agagaaatta
    18901 taggatatgc aattcaaaaa tgtgcagcag atcacgtagc cgaaactact gtaacagtag
    18961 taagcttgcc taatgatgaa atgaaaggta gaataatagg tagggaaggt agaaatataa
    19021 ggactttaga aaccttaaca ggtatagacc ttataataga tgacactcca gaagccgtaa
    19081 ttttatcagg atttgatcca ataagaagag aaattgcaag aattgctcta gaaaaattaa
    19141 tagcagatgg aagaatacat ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag
    19201 aagttgacaa tattataaaa gaatatggtg aacaagctgc atttgaaact agtgtacatg
    19261 ggattcatcc ggaattaata agattattag gtagacttaa ctatagaact agttatggac
    19321 aaaatgtact taaacattct atagaagttg ctcatatagc tggtataatg gctgctgaaa
    19381 taggagcaga tattagattg gctaagaggg caggtttatt acatgatata ggtaaggctg
    19441 tagaccatga aatggaaggt acacatgttg aaataggtat ggatttactt agacgttata
    19501 aagagtctaa agaagttata catgccatga gtactcatca tggagattac gagcctcaaa
    19561 cagtagaggc tgtattagtt acagcagcag atgcaatatc agctgctaga cctggtgcga
    19621 gaagagaaac tttagaagca tatataaaga gacttgaaaa attagaagaa atagctaatt
    19681 catatgaagg tgtagaaaaa tcatttgcta tacaagctgg tagagaaata agaataatgg
    19741 taaaaccaga agctataaat gatgaagaaa tacatctatt ggctagagat atgactaaga
    19801 aaatagagga tgaacttgaa tatcctggac aaataaaagt gagcataata agagaaacaa
    19861 gagctataga gtatgcgaag taaaagaaat aatatctaaa taataagaaa atatttttag
    19921 aaatttaaat ttgccacaag atatgataat atatcttgtg gcttttaaat tgtttaagtt
    19981 taattttata taatgcaaaa ataatctaag tgctaaaagt gctacttaca aaagattaat
    20041 aaaataaaga tttatatatg gattatttat ataattatcc aagtttgttt gatataatga
    20101 agttgtaaaa taaatatatt actacttgga atgcttgtac ttaaataaga tgaaaataca
    20161 atgacgctaa agtgaggatt agagagaggg tacactatga aaaaaaatac acttttacat
    20221 acaaatatta tggtttgcat tattattact cttggttttg tggctacctc tataattgga
    20281 tatcaatcta ataccaatat ttttaaaaat gatattgaac atgtatctac attggctgct
    20341 gaaggtattt attatcagat agataaacta ctttcagaac ctataaatgt atctcttaca
    20401 atggcaaatg acagtctatt aaaaaatttt ttagatggtg agaaagaaca tttaaatgat
    20461 gaagaattta tttataaatt acaagattat ctgaatgtgt atagaaataa atattcatat
    20521 gattctgttt ttttagtatc aactaaaacc aataattatt atcattttaa tggacttgat
    20581 agaaatctta gcgctaataa ctcagagaat caatggtatt atactttctt aaagaatgat
    20641 gatgaatact cattaaatgt tgataatgat gaagcaagta acaatagtat tacagtcttt
    20701 gtaaattgta agataaaaga tgataatgga gcaactatgg gaattatagg tgttgggtta
    20761 aaggttaatt cattacaaat gttgcttaaa ggatataatg acaaatttga tgttgttgca
    20821 cgcttaattg atgataaagg atttgtacaa ttagcagtag ataaaaccgg tcatgagaat
    20881 ataaattttt ttgaaaattc tagtagcgac ttgtctaatt cgaaacaatt aatattaaac
    20941 aacaaaaagg aacaaaagag tttttggtat tcatctgaaa aatcaaaaag ttatattgta
    21001 tgtcagtaca tacctagttt aaaatggcat cttgttctag aaaatgattt tactttaatg
    21061 attaaacagc ttcatctgca attttttaaa ggcattgcag ttatagtttt aattataata
    21121 tttgtacttt ttactattac acatgtgctt aaaaaataca aaaaccaaat aataaagctt
    21181 agtgtttcac agaaattgga atatcaaaaa ttgttgagca aatctactga aggtctttat
    21241 gaaaatattt ttgaaattga tattactaac aataaagctg gttgtgaaga tgagaatagg
    21301 tattttcatg aatttggatt agggcttaat accccatatg atgaagcctt acatacaata
    21361 gctaaaaacc aaatcaaaga agaatatgca caaggttatt tagatacatt tttacctgaa
    21421 aatgtaatta aacgttataa tagcggaatt aataatatca gctatgattt tttaattaaa
    21481 aaagatgaag aaagttatca ttggattaga attaatgcac gaattttctt ttggacatca
    21541 gatgaatctg ttcgtatgat atcttatcgt aaaaatatag atgaagaaaa aaatagagaa
    21601 ttactacttc tcgaacgttc acaacgtgat tctcttacag ggctttacaa taaagctaca
    21661 acagaggaat ttattcgttc atttttaaaa aatgaaagta atcaagattt agaacacgtt
    21721 tttctaattt tcgatattga taattttaaa aatgtaaatg ataaatatgg tcatgtattt
    21781 ggagactttg ttataagtga atttgcctta gaattaaaat ctcaatttcg tgaggacgat
    21841 attgtgggac gtattggtgg agatgagttt gtagtattaa taaaagactt taataataat
    21901 aactatgtgc ttatagacaa attagagcgt ttttgttcta agattaatca gaaaaaattt
    21961 gttgagcaag cagaattttc tattgcatgt agtattggtg tagcaatgta tcctgaacat
    22021 ggatatgtat actctgaact atatgagaaa gcagaccaag cactttacta tgcaaaaagc
    22081 catgggaaaa attcttatca tatattttca gaggattcta ttgatagtat ttcatttcat
    22141 atagactcta gagatataaa agtattatta gaaatggcta ctgatggtgt tgtcaaattt
    22201 gcttgtatgg aaaacttaaa attactttat ttcaaccaaa aaatgcttga atttactgga
    22261 tattcagctc aaactgttac ttctataggt tttgatattt tttcgcctgt attacaagac
    22321 gacttacaaa tggtactaga tacattaaga gaagcaattt ctaaaagaga gacttttgaa
    22381 atagtttttc gtttttgtca tacagatggt catatttttc ctgtaaggat gcaaggtaag
    22441 tttgtaagtg aattatatca aaatcgttat cctgtttttt atgcgattta tacagacata
    22501 tcaagtatca atactttact agaaaataaa tagtaaatac tacagtaaaa tctaaaaata
    22561 ggatataata gtattaatat aagaagatta taaataaaac ctgctaatga aaatagatta
    22621 tttttagcag gtttttaaat tttttataat aaatcttatt tttggctaac aagatttatt
    22681 ttttttaaga ctttacttat tattttaagc attaattatg aaatttatca ttttattatt
    22741 tacagaaagt attttataac aataaatatc tatacttatt gcaaaattat tttagcagtt
    22801 ttgtatttat ttgtatttat tttgaattta tttactaaat ttgcctataa aaagtatata
    22861 taaaaatata gttcaaaatg gtataattaa gtataacaga aggaggtaat gtaagtgagc
    22921 caatacataa atataaattc tcagttagac gccctaaaat tgaaggagtt cgagaaaata
    22981 ttttacgaaa atatcaatga ggacgttaaa gtagtgccaa aagttactcc ttttaaagga
    23041 ataaataccg atttgttgta tgttaaagat ggaagagttt tatttataaa atttatggat
    23101 acaacagaag atatattttt tattttagaa gaagagttac ttgaagttat gaatgaggaa
    23161 tatgaacttt tgaaactaaa aatgggacaa aagaatagaa atataagtta taactacgtt
    23221 tacattatgc catacgttga agttgaggaa acatatgaat ttgaagaatt tgttaataat
    23281 aatataatag ataaaaataa gcttcaagat ataatgaata agggttcgct ggaggaatat
    23341 cttaatgatg agaatgatga aataaactta aatttatttt tactagacat atgttcagaa
    23401 tactatataa taaatgataa acttcatcta aatgaaaaat ttaaaaaaat ttcattctac
    23461 aatgatgact ataagtatac agcaactatg atggaagacg ttcaaattaa agatgttgtt
    23521 tctattaaat atgggaatac acttattgaa ggtggttctg gtataggcaa aacagcaatt
    23581 atgctttcta gggctataaa attagcaaaa gtatatccac atcataaatt agtcatattt
    23641 acacatacaa aacaattgtg caatgagctt cgagagagga tagagttatt atacaaagat
    23701 aataacaatt tagaagttca cacatttagt tcttttatat ttaaactagc taaaaaattc
    23761 aatttaattg tagattataa catgttaaaa aatgattacg aaaagacctt taataatctt
    23821 gtaaaacaag cacagaacat tataaaaaat aaaaatatgt tcaaggctat ttttattgat
    23881 gaagcagaaa gctttttaga gtatgaaata gattttataa gggagttttt atataaaaca
    23941 aaattcatat ttaatgtgtg ttcttgtaac tccttgaata tctctaatag attgaatata
    24001 tttaaaaaat tatataatgg tatagaattt gatgataaga ttatcttaag taaaaattat
    24061 agacaagcta aagagattgt agattttaca aataaatttt caaataattc taactcatat
    24121 atcaacgaat taagaccaaa tacagaattt agtactttct tttatactaa agcacttaga
    24181 ggtggaaata agtctgttga tataataaaa gtaagtgacc ttgatgacca aataagttct
    24241 gtaatctggg aaattgagta tttaataagt aaaaaaggtc ttgattattc tgaagttgct
    24301 atcgtttatc cttacaataa gaagaaatta aaaagtggaa aaaccattta ctttcaatat
    24361 atgttaaaaa aagcattgga agaggcaaaa attccttata tatgtgcaga agataatttg
    24421 acaaatataa gtaagaaggt aggaactaca atagcaaata tatatgcaat aaagaattta
    24481 gagtataaag cggtaatagt atgtgaatta gagatgttat acaatcaaac tataaatgat
    24541 attgaacaag attatcaagt aaatgacttt gtaggagatt taaataaggt ttatttagct
    24601 atgagtagag caacagacta cctaagtata gtaacaactt ttaatgaaga agcaagtgat
    24661 ataataagac ttattacaga atcaaaagat atttagtaaa aaaatatttg taatttaaga
    24721 aaataaaaag agactgagat ttgtaaaata aactttacca attcagtctc ttatttttta
    24781 gtctactttc ccaatattat caagaggtaa tagtctcacc ataaccttac caagaacttc
    24841 atcttcatca accataccta catcagggaa tctactatca ttactatttt ctctattatc
    24901 tcccattgca aatagtttac cttttggaac aacagtatct atatctccag aagtatagtt
    24961 attgtgtata tatggttcat ttaataattt tccatttaca tacactttag aatttgatat
    25021 ttttattctg tcaccttcag tagctataac tctttttatt aagtcttttt tctttcctgt
    25081 ttctccatca actaaattgg ttttaaaaac tacaatatcg cctttttcag gtttaccaac
    25141 cttgtatgat atcctattta aaatcagata atctttatca tctaaagtag gatacattga
    25201 ttctccactt acaatagaag gtcttataaa ttgtagaatt acaaatgcaa gtacaatagc
    25261 catagctatt gacttaatcc aatcaaatat ttctttttta acactcataa cttttctcct
    25321 tatcatttgt tattaaatta gaatacatta taatagaaag aaataagcaa gaaccactat
    25381 acccaagact attctataat aaccaaatac tttgaagtca tgttttttga tatagtccat
    25441 aaaaaattta ataactacaa ttgaaacaac aaatgctact acagaaccaa cacctaaaat
    25501 aagccattca aatccagtaa atccaaatcc agcttttaga agttttaatg cactagctcc
    25561 aagcattgta gggatggcta agaagaatga aaactctgct gctacatatc tagaagtacc
    25621 aagtaatact gcacctatta tagtagaacc agacctagat gtacctggta taagagctag
    25681 acactggaat aatcctatac aaagagctaa tttatatgta acttgagaaa aatctttata
    25741 cttaggtttt ttatttctct tttcaagccc tatcattatt ataccatata caataagagc
    25801 aatagcaact actgttgaat taaagaataa tctatctata tcatcttcaa acagtattcc
    25861 aataactcca gatggtataa ctgcaattat aaccttaagc cataaacgga ctgtttcttt
    25921 tttctgctta atattctttg cgctatcaaa aggatttaat tttctaaaaa atataacaag
    25981 tacagctaat attgaaccaa attgaattac aactaaaaat gtacttataa atgtatttga
    26041 aaaattcaat tttataaact catcaactaa tatcatatgc cctgtactac ttactggtag
    26101 ccattctgtt attccctgaa ctatgcccaa aatgactgcc ttaaaaattt caagcaaact
    26161 aaatgtttcc aaatcaaaat acctccaata caaatttata gtagcatatt ttattcatag
    26221 attaataaaa aaatgccacc ttttactaac ttataatacc atagtactgt gtagtgcaca
    26281 atggaagatt gatattttca taaaaccttc ataatataac aaaataaact taaaagttat
    26341 ctattgaata aggggtatat ttgaggggga aatatatggt atagtaaaag tatagctatt
    26401 ttattttatg aaaaggaaag gagttattat gtatagtaca aaaatactag atgattatga
    26461 gtatgaaact tttaatgttt tttctacaaa attaaatcca aacacgaaac acgattattt
    26521 atcaaaggtt atactgttta aagaattttt aaaagggaaa gaattgattt atgctacgaa
    26581 agaagattgt aaaaattttg tagactatat acagactaaa tatgcgaaat caacttgtga
    26641 aaagatatat agttatttac atagttttta taacttctta aaaaaagaag gatacataga
    26701 tataaatcca tttagatatg tagaaaaacc cacagtaaca agaataaaaa caaaagatga
    26761 tgttctaagt atacaagaaa taaataaact tataggtatc ctaccaaaat taaatataag
    26821 agatagagta ataatagtct gtctagttac tactgggtgt ttattaaatg aattagttag
    26881 cttaaagtgg aaagacctta tggtagatga aaacgataac tcatatgtaa ggttaggtaa
    26941 gggaagaaaa gaaagagtag ttaaattaca tccatacttt tttaaacttt tagaagatta
    27001 caggaattat tctgggcttc cagaggtaat aatacctagt gatgatttta tttttacaac
    27061 tcaaaagagt aattctataa cagatagaaa tgtcagactt atagtcaaaa aagcattaga
    27121 tttagctgga ctttctcaat attcagcaag agattttaga cattcatttg cagcaataag
    27181 tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag cttggatgga gtgataaata
    27241 ctatgcaatt agatataagt atgtattaaa ttttgttgat agtgaaatag tggattactt
    27301 aattgaaaaa gataatttgg atataaataa aaaatagtaa taaataaaaa atactatata
    27361 taaatgtgaa caaaaaataa aaaag
//
LOCUS       DAFKMX010000032        27178 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFKMX010000032 DAFKMX010000000
VERSION     DAFKMX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.032"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(359..1834)
                     /locus_tag="LZB08_001132"
     CDS             complement(359..1834)
                     /locus_tag="LZB08_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HBM7314644.1"
                     /translation="MNYNKKNEVLNLIQILIEGIDYIKNNNLSESIVNSSLEAINYMK
                     KYINNSGRSNKNLLNLIDETYVKILRLSMYKNIRLIEDCELIISNLNYLSNIIENSLN
                     KKTKIVFMPYNAKMWNSLESIWKSAVLDEQCDCYVVPIPYYKLIDTPNGITQIYTYEG
                     NDFPEDVPVIHYDDFDLSKEKPDIIYVHNQYDDCNNATMVDSNYFSYNLKQYTNMLVY
                     VAYGILGTYPVSFYLNFYELIASRNFDKVIVQSPAFEIIAECSGINKNQILTMGSPKF
                     DSLIYNLKQKNINKNYESKLKGKIIFLWTTNLMKIPNGKDGVIDEIENVFDIIENSQE
                     YGLIYRPHPLELEYVKSKVPECFNRYKTLLDSISIKNNIILDDSVSYYESFNLSHALI
                     TDRSSVLIEYIQTKKPILIYDIDMERGYYDSRIFDIFSNYVVGEEDMDLIKFMNLVKN
                     NNDYKLNQRLNSLNSVLSNTDGSCGEKIHTNVLEYVLNNHI"
     gene            complement(1872..2612)
                     /locus_tag="LZB08_001133"
     CDS             complement(1872..2612)
                     /locus_tag="LZB08_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7314645.1"
                     /translation="MGNATRIYEKHVDIKKDTVQDFWNKRAKKHIPENPYISVKCNDQ
                     NPDYAKNLDKYEKEIIIPKLNITRNSNILDIGCGVGRLADDIVPICNYYLGTDFAEDL
                     ISIAKKRFSDNDCDFQVCDFINTLYDTKVKSKMPFDTILLAGVTMYINDDELKIALKN
                     LLGILDKKATIYISTPIGINKRLTLKEFYSKELNSEYNVIYRTIEEYEEILKPLLDEG
                     FEIVESKSFLTDINQYSDTERHYFILKR"
     gene            complement(2625..3269)
                     /locus_tag="LZB08_001134"
     CDS             complement(2625..3269)
                     /locus_tag="LZB08_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBM7314646.1"
                     /translation="MPTTKTIVVNCAGRGSRLGLNSNKSLIDIDGKPLIIRQLECLDD
                     YDDIRIVVGFQAEKLIEVVCSYRKDIMFVFNHDYLNTKTAASLSLGKKYSRDTVISLD
                     GDILFFKEDFDNFVNTDGEIIGVCQPYTKDPVYVKTDFINDGEFATEFSRTNGDYEWT
                     GLFKIYSKNLSSGTNHVYKMISPNLPLKIAHVELREIDTPEEYNEALKWYRNKK"
     gene            complement(3242..3859)
                     /locus_tag="LZB08_001135"
     CDS             complement(3242..3859)
                     /locus_tag="LZB08_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HBM7314647.1"
                     /translation="MKGNEMENYKFVFDLDSTLTKQEILPEISKCINKYELMQNLTNE
                     TMLGNLSFEESFKKRVDLLKCIPISKVKSIVAKINLNEKIVKFIKENPDRCTVVTNNL
                     DIWICDLMKELSLENKYYSSIAHSNGDFIDKIKVIIKKEDIIKKMKGPIVAVGDGSND
                     IKMIENADIGIGFGGVRPIAPGILKVCDYAFYEEERLCQLLKQLL"
     gene            complement(4561..6702)
                     /locus_tag="LZB08_001136"
     CDS             complement(4561..6702)
                     /locus_tag="LZB08_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBM7314648.1"
                     /translation="MLLSIVMMVKNEENILEKTLKSLSVLRNSIESELIIVDTGSTDN
                     TIRIAKQYTEKVYFHNWNDDFSSMRNISISYAKGEWLLILDADEVLIDYSNIIKFFES
                     GLNKKFNSASVRLKNLYSFDKKLYGYCSVLRFFKNLDFKYCGKVHEQPMYKNPIFNNI
                     ADFEHYGYLFEDEEIRINKVKRNEKLLLEELKEDEKSPYTNYQIGKNLIILGKYQEAL
                     DYLEKSYKLYSKLEIVPGYLITNLAKVYLYLGKAKKCEKLCLKYINKDRNNIDIYYHI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEMSTQANSLLSDTDAVGFRDEAIITLIKIYY
                     KLEKYDLVISEYDNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYREIPNSKVERDSF
                     YKSMEDFINNIKDDEKELIYKSLCDIEGNYGLLNKIRINKHVSLDTCRKILNEEKDIV
                     YASVIKIAFEEKVDLLDIFYNMEYVWIEKYLRYILALERSFNVSIYKYIISCPNTSEI
                     EKIRVYKVISKILLESVNLSDKKYKELFYIYIMYSYKYIKYIYSNLSDCELIKFVCND
                     IDKFTLEFKHVLDGKRKINNPNEKLEYLHNMRILLNEYPFYNRIIKIFISELEESITE
                     SEEFKLLKDNFIIRIENMIENGKINDAKLLIEDYSKTFADDVKILNIKAILFMCEDKF
                     EEADFALKKALSLDLENEDTIYNIEYLKNLK"
     gene            complement(6796..7668)
                     /gene="fliC"
                     /locus_tag="LZB08_001137"
     CDS             complement(6796..7668)
                     /gene="fliC"
                     /locus_tag="LZB08_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBM7314649.1"
                     /translation="MRVNTNVSALIANNQMGRNVNGQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGSLEETGNILQRMRTLSLQSANEIN
                     NTEEREKIADELTQLKDEIERISSSTEFNGKKLLDGTSSTIRLQVGASYGTNVSGTSN
                     NNNEIKIQLVNTASIMASAGITTASIGSMKAGGTTGTDAAKTMVSSLDAALKSLNSSR
                     AKLGAQQNRLESTQNNLNNTLENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(7769..8104)
                     /locus_tag="LZB08_001138"
     CDS             complement(7769..8104)
                     /locus_tag="LZB08_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBM7314650.1"
                     /translation="MDKLEEKINLYKDISLKIINFIEKMEYKNISFQLDERQNIINSI
                     SEVDKSEFIQLYDSMELFEIDAKIRDALQEQLSEVKKELHEYKLTKQVNTMYYSSNRE
                     KVNIFNKKV"
     gene            complement(8107..9630)
                     /gene="fliD"
                     /locus_tag="LZB08_001139"
     CDS             complement(8107..9630)
                     /gene="fliD"
                     /locus_tag="LZB08_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBM7314651.1"
                     /translation="MSSISPVRVTGLSGNFDMEGIIEASMIRDKEKVNKAKQDQQIVK
                     WKQEIYRDIIKESKNLYDKYLNGDSPNSITNKKAYSATRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKVTIKLNSSDPIVQQFPPNASGASSLNIGGVNIPISEQDTTSTI
                     VSKINSLCADNDIRASYSEMTGELIISRKQTGSSSDIDLKVIGNDSLAGQIASDNGIT
                     FTTDASGTKSAVVYGKNLEADVTDDQGRVTHISKEQNSFKIDNIDYNVNSKGSAKLVS
                     VTDTEEATKNMKAFVDDYNALMDKVYGLVTTKKSKDYPPLTDEQKDDMTTEEIEKWEK
                     KAKEGILRNDDELRAFVEDIQSMFFGDADTIIALRKLGISEHENYNKKGQISFNADTF
                     SKALIDDSDKVYKALAGYSSNYDDKGMFEKLKKIVFEYSGSSASKLTKKAGMENSSSA
                     SQNVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNKYSSQMNYFSQAQGN"
     gene            complement(9658..10008)
                     /gene="fliS"
                     /locus_tag="LZB08_001140"
     CDS             complement(9658..10008)
                     /gene="fliS"
                     /locus_tag="LZB08_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBM7314652.1"
                     /translation="MELDLNELNGLSKEELLLMLVDGTVKYTNISKVALLNKDYLKAH
                     NELVRVQNIFTELMVTLDQSVGQWAKDMYKVYEFIKHELVKADINKDIKIIDDILPIV
                     EQIRDTWHEVYNKL"
     gene            complement(10008..10403)
                     /gene="fliS"
                     /locus_tag="LZB08_001141"
     CDS             complement(10008..10403)
                     /gene="fliS"
                     /locus_tag="LZB08_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBM7314653.1"
                     /translation="MYGENPYNSYKQNAIFMASKEQLLLMLVDGAVKYTKIARGAIID
                     KNTRKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDVQII
                     DNVLPVIEEIKDTWHEADRKSREERSRYK"
     gene            complement(10425..10637)
                     /gene="csrA"
                     /locus_tag="LZB08_001142"
     CDS             complement(10425..10637)
                     /gene="csrA"
                     /locus_tag="LZB08_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBM7314654.1"
                     /translation="MLVISRKKDEAVLIGDNIEVKVVGVDGNNIKLAISAPNNISILR
                     KEIYEKVKNENIKATNKNIKILKSLK"
     gene            complement(10631..11023)
                     /locus_tag="LZB08_001143"
     CDS             complement(10631..11023)
                     /locus_tag="LZB08_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBM7314655.1"
                     /translation="MMKITLKKGILGFENLKEYELLDIENEDILKEFNSTEEDCIGFI
                     VVSPFEIIKEYEIVLNQETIEKLEVKSPNDIMLLNIITVGQTLEESTVNMKAPIVINV
                     RNNCGMQIILQDEEYSIWHPLLRGDGGC"
     gene            complement(11039..11971)
                     /gene="flgL"
                     /locus_tag="LZB08_001144"
     CDS             complement(11039..11971)
                     /gene="flgL"
                     /locus_tag="LZB08_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBM7314656.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQINTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYIENCKDTADWLETTDTSLDQLGNLLADIKKGLVSSGNGSY
                     SDDEIKTISNSTNEKMKEIANALNATHEGKYIFSGSNTGTPPVECVENADGSVSLKFN
                     TSLNLNKLNDSLSVDVAQGISVDYNVKLSSLGFDPTKTDPFEKLNNISKKLANPSDAN
                     IKELTTTCLGDMENLIDNTVNVRSIYGTKANTVDAMKEKNDEGLIQLKDVLSQNDEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(11990..13300)
                     /gene="flgK"
                     /locus_tag="LZB08_001145"
     CDS             complement(11990..13300)
                     /gene="flgK"
                     /locus_tag="LZB08_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBM7314657.1"
                     /translation="MAGLFSILNTANSGMNAHQKSIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNSSAGITVRDFFITSNVAGEDIIKVNPAIKSNSNELKLTTEEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNVDNSR
                     MSVSGVSLDEEMTELMKVQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(13318..13734)
                     /locus_tag="LZB08_001146"
     CDS             complement(13318..13734)
                     /locus_tag="LZB08_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBM7314658.1"
                     /translation="MITDLKVIIYEEKKIISNMMELLKEQFDYIIEKDLENLNRLNPK
                     LEDISRELASIEIKRRQLLGDDVSISEVVENSNDEYLKEIYIDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(13739..14014)
                     /gene="flgM"
                     /locus_tag="LZB08_001147"
     CDS             complement(13739..14014)
                     /gene="flgM"
                     /locus_tag="LZB08_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBM7314659.1"
                     /translation="MNIKSVSSNIVVNNYLKNKSDVKKVQQVQKVDKTDKIELSKFAK
                     EMSSVSNMSDEAARNKKIEEIKSALENGTYKIKPEEIAKKMIEEMRG"
     gene            complement(14165..14479)
                     /locus_tag="LZB08_001148"
     CDS             complement(14165..14479)
                     /locus_tag="LZB08_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBM7314660.1"
                     /translation="MNSKKEDIYELNFGQLIEEKTGIIEENQNVFNAKVNVAVSIGNI
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNETLSVRLSKIEDSD
                     VN"
     gene            complement(14503..14931)
                     /locus_tag="LZB08_001149"
     CDS             complement(14503..14931)
                     /locus_tag="LZB08_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBM7314661.1"
                     /translation="MGINIDKLNISPSRSIEVKKTKENNKFSDSLNFVKQSSSLEDIK
                     RNLDGVKKAGERLVLTQNYGDISRYKNAVKEYLKSVVDNMYSLDKSSSFWEHQYYKNV
                     KIIDNKLEDMTSKLLSEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(14940..15608)
                     /locus_tag="LZB08_001150"
     CDS             complement(14940..15608)
                     /locus_tag="LZB08_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBM7314662.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCGYNNSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSEKLFNELDSVANNTVGKFIEVDTKDKSVKSRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTNPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMRSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(16054..21135)
                     /locus_tag="LZB08_001151"
                     /pseudo
     CDS             complement(16054..21135)
                     /locus_tag="LZB08_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(21146..22678)
                     /gene="purH"
                     /locus_tag="LZB08_001152"
     CDS             complement(21146..22678)
                     /gene="purH"
                     /locus_tag="LZB08_001152"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBM7314663.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(22671..23264)
                     /locus_tag="LZB08_001153"
     CDS             complement(22671..23264)
                     /locus_tag="LZB08_001153"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBM7314664.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGI
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(23258..24334)
                     /locus_tag="LZB08_001154"
     CDS             complement(23258..24334)
                     /locus_tag="LZB08_001154"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBM7314665.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(24328..25695)
                     /gene="purF"
                     /locus_tag="LZB08_001155"
     CDS             complement(24328..25695)
                     /gene="purF"
                     /locus_tag="LZB08_001155"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBM7314666.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(25707..26405)
                     /locus_tag="LZB08_001156"
     CDS             complement(25707..26405)
                     /locus_tag="LZB08_001156"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBM7314667.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(26411..26887)
                     /gene="purE"
                     /locus_tag="LZB08_001157"
     CDS             complement(26411..26887)
                     /gene="purE"
                     /locus_tag="LZB08_001157"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBM7314668.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
BASE COUNT         8210 a         4900 c         2747 g        11321 t
ORIGIN      
        1 aatttacaat ataaaataaa tttattctaa atttgtcttt tacaataatt tcattaaaag
       61 tatttatttt aacattattt cccaattata tgcttaggat tttatatcta tttaaagttt
      121 tcccttttat tttcactttt agggataaaa aatttgtaat tttctctact tttgataaat
      181 tttattttag actggttata taagaataca gtctaaaatg ttgtatagag taacaaaata
      241 aatttaaaaa aggtcaaact ttaattaatt tgccctttat tatcttatat ttattaaaat
      301 tgataatgtt ttaactcctt tccaagccta catttatcta attattaatt aattttaact
      361 aaatatggtt atttaaaacg tattctaata cattagtatg tattttttca ccacaacttc
      421 catctgtatt tgatagtaca ctatttaaag aatttaatct ttgatttagc ttataatcat
      481 tattattttt tactaagttc ataaatttta ttaaatccat atcttcttct cctactacat
      541 aatttgaaaa tatatcgaat attcttgaat cataatatcc acgttccata tctatatcat
      601 aaattaatat aggtttcttc gtttgaatat attctattaa cacagaagac ctatctgtta
      661 ttaaagcatg ggataagttg aatgattcat agtaagatac agagtcgtct aaaataatat
      721 tattttttat agatattgaa tctagcaaag ttttgtatct attaaaacat tctggaactt
      781 tagattttac atactcaagt tctaatggat gtggtctata tataagacca tactcttgac
      841 tattttcaat aatatcaaat acattttcaa tctcatcaat gacaccatct ttgccattag
      901 gtattttcat tagattagtt gtccataaga aaattatttt accctttaat ttagattcat
      961 aatttttatt tatattcttc tgttttaaat tataaattaa agaatcaaat tttggagatc
     1021 ccattgttag tatttgattt ttatttatac cactacattc tgctataatt tcaaatgcag
     1081 gagattgcac aataacctta tcaaaatttc tagatgctat caattcataa aaatttaagt
     1141 aaaaactaac aggataagtt ccaagtattc cataagcaac atatacaagc atattagtat
     1201 attgtttcaa gttataagaa aaataattac tatctaccat tgtagcattg ttacaatcat
     1261 catattgatt atgaacatat attatatcag gtttctcctt agataaatca aaatcatcat
     1321 aatgtattac tggaacatct tcaggaaaat cattaccttc atatgtatat atctgagtta
     1381 taccattagg cgtatcaatt aacttgtaat aaggtatagg aactacataa cagtcgcact
     1441 gctcatccaa aaccgcagat ttccagatac tttctaaact attccacatt ttagcattat
     1501 aaggcataaa tacaatttta gtttttttat ttagactatt ttctattata tttgataaat
     1561 agtttaaatt acttatgata agttcacaat cttcaataag tcttatattt ttatacatgg
     1621 aaagtcttaa aatttttaca taagtttcat caattaaatt caacaagttt ttattgcttc
     1681 ttccagaatt atttatatat tttttcatat aattaatagc ttcaagagat gaattaacta
     1741 ttgattcact taaattatta ttttttatgt aatcaatacc ctctataagt atttgtataa
     1801 gatttaaaac ttcatttttt ttattatagt tcatcagatt caccttaatt ttgattttat
     1861 ttatattaaa actatcgctt tagtataaaa taatgccttt ctgtatcaga gtattgattt
     1921 atatctgtta agaagctttt gctttcaaca atttcaaatc cttcatctag taatggtttt
     1981 aaaatttctt catattcttc tatagttcta tatataacat tatattctga atttaattct
     2041 ttcgagtaaa attctttcaa agttaatctc ttatttattc caataggtgt agatatatat
     2101 attgttgcct ttttatctaa tattcctaat aaatttttca atgctatttt taattcatca
     2161 tcatttatat acattgttac acctgccaat agaattgtat caaatggcat tttggattta
     2221 acttttgtat catatagagt attgataaaa tcacatactt gaaaatcaca gtcattatca
     2281 gaaaatcttt ttttagcaat agatattaag tcttcagcaa aatctgtccc aagataatag
     2341 ttacatatag gaactatatc atcagctaac ctacctaccc cacaacctat atccaatata
     2401 tttgaatttc tagttatatt tagtttagga attattattt ctttttcata tttatctaaa
     2461 tttttagcat aatcaggatt ttggtcatta catttcactg aaatataggg attctctgga
     2521 atatgctttt tagctcgttt attccaaaaa tcttgaacag tatctttttt tatatctaca
     2581 tgtttttcat aaattctcgt tgcattaccc atataaaatc tcctttattt tttgttccta
     2641 taccatttta aagcttcatt atattcttcc ggtgtatcaa tttccctcaa ttcaacatga
     2701 gctattttaa gtggtaagtt gggtgaaatc attttatata catgatttgt accagagctt
     2761 aaatttttag aatatatttt aaacaaacct gtccactcat aatctccatt tgtccttgaa
     2821 aattctgtag caaactctcc atcattaata aagtctgttt taacatagac agggtcttta
     2881 gtataaggtt gacatacacc aataatttct ccatctgtgt taacaaaatt atcgaaatct
     2941 tctttgaaaa aaagtatatc accatctagt gatataacag tatctctaga atattttttg
     3001 cctaaagata agcttgcagc agtcttagtg tttaagtaat catgattaaa tacaaacatt
     3061 atatctttac gataagaaca tacaacttca attaattttt ctgcttgaaa gcctactaca
     3121 atacgtatgt catcataatc atcaagacac tctaattgtc ttattattaa tggctttcca
     3181 tctatatcta ttaacgactt atttgaattt aatcctagtc tacttcctct acctgcacag
     3241 tttacaacaa ttgttttagt agttggcata aacgttcctc ctcataaaat gcataatcac
     3301 atacttttaa tattccagga gctataggtc ttacccctcc aaatccaatt ccaatatcag
     3361 cattctctat catttttata tcattactgc catcaccaac tgcaactata ggacctttca
     3421 ttttttttat aatatcttct ttttttatta taactttaat tttatcaatg aaatcaccat
     3481 ttgaatgagc tatagatgaa tagtatttat tttctaaaga aagctctttc attaaatcac
     3541 aaatccaaat atctaaatta tttgtcacaa cagtacatct atctggattt tcttttataa
     3601 actttacaat tttttcatta agattaatct tagcaactat acttttaact ttagaaatag
     3661 gaatacattt taataaatcg actcttttct taaaactttc ctcaaaagat aaatttccta
     3721 gcatagtttc atttgtaaga ttttgcataa gttcatattt atttatacac ttagaaatct
     3781 caggaagtat ttcttgttta gttaatgttg aatctaaatc aaaaacaaat ttataatttt
     3841 ccatttcatt ccctttcatt gaagcataaa atcctatttt tgagtgataa gtacatcaaa
     3901 caatattgag ttagaaattt caataatttt acagagttta taattttata atataatttt
     3961 attttttatt ataaaattgt atatagctca atcaaaaagc taaaaactaa atcttatcaa
     4021 agtaaagaaa tcttgcgttc ttaactaaca aaaatactct ataaatggtt tttaagtatt
     4081 aaaatgactt aataaaaatt caattattac aaatactgct gttttatgtc gaattaattc
     4141 aattatttaa aaacactaaa atcattgaaa tcactaaaaa attgtcttaa tgtgcaaaac
     4201 cttcgttttt aggatattgc ccgatttaat tatatacata ttatcgtcta attttaaaaa
     4261 aaattaagag aaaatatgta taattttcat ttatattgtt ttttgttgtt tattttttga
     4321 acatgcttat ctattttatt attataagta aatatgataa aattagtttc ccttgaatta
     4381 aatcttctat atactaaaat cattgagtat attctatttt atttgatgcc aatatcgata
     4441 tatattgctg taccagtaaa ttttacatca gtatttttat atgtatatat tcaatcttat
     4501 attatatttt tcattaactg ttattgatat tattattttc taaataacct agttttaaat
     4561 ttattttaaa ttttttaagt actctatatt atatatagta tcctcatttt ctaagtctag
     4621 tgataatgcc ttttttaatg caaaatcagc ttcttcaaat ttatcttcac acataaacaa
     4681 aattgccttt atatttaaaa tctttacatc atctgcaaat gtttttgagt agtcttcaat
     4741 caataacttt gcatcattta tttttccatt ctcaatcata ttttctatac gaataataaa
     4801 gttatctttt aataatttaa attcttcact ttctgttata ctttcttcta attcacttat
     4861 gaagattttt attattctat tgtaaaaagg gtattcattt aataatattc tcatattatg
     4921 tagatattct aatttttcat taggattatt tatcttcctt ttaccatcaa gtacatgctt
     4981 aaattctaaa gtgaacttat ctatgtcatt acaaacaaac tttattaatt cacaatcact
     5041 caaattgcta tatatgtatt ttatgtattt ataagagtac attatataaa tataaaaaag
     5101 ttctttgtat tttttatcac ttaaattaac actttctaaa agaattttag atataacttt
     5161 gtacactctt atcttttcta tttctgatgt attaggacaa ctaattatat atttatatat
     5221 tgatacatta aaacttcttt ctaaagctaa aatatacctt aagtatttct ctatccaaac
     5281 atattccata ttgtaaaaaa tgtctaataa gtcaactttt tcctcaaaag ctatttttat
     5341 aacacttgca taaactatgt ctttttcttc atttagtatt tttctacatg tatctaatga
     5401 tacatgtttg tttattctta ttttattcaa aagaccataa ttaccttcaa tatcacaaag
     5461 gcttttatat atgagctctt tctcatcatc ttttatatta tttataaagt cttccatact
     5521 tttataaaaa ctatctctct caaccttcga atttggaatc tctctataat agttttttat
     5581 gtcttcaaat ctatttaatt tatataaaga cataaataag ctaaagtaca catttttctt
     5641 tttttccaca tcttcaatat tatcatactc tgaaattact aaatcatatt tctcaagctt
     5701 ataataaatt tttatcaatg taattatagc ttcatctcta aatcctacag catcagtatc
     5761 tgaaagcaaa ctatttgctt gcgtactcat ttcataatta tctagcaaat aaatatatct
     5821 tttatatgaa tctatactat tttcatactt ccctaaatca acttgtgctt gtgcaatatg
     5881 ataatatata tcaatattat ttctgtcctt attaatatat tttaagcata atttttcaca
     5941 cttcttagcc tttcctaagt ataaatatac ttttgcaagg tttgttatca agtatccagg
     6001 tactatttct aattttgagt ataatttata agacttttct agataatcta aagcttcttg
     6061 atattttcct aatataataa gatttttccc tatttgataa tttgtatatg gagatttctc
     6121 gtcttctttt aattcctcaa gtaacagttt ttcatttctt tttactttat ttattcttat
     6181 ctcttcatct tcaaacaaat atccataatg ttcaaaatca gcaatattat taaatatagg
     6241 atttttatac attggttgtt catgaacttt cccacagtat ttaaaatcaa ggtttttgaa
     6301 aaatctcaac actgaacaat atccatataa ttttttatca aatgaataaa gattttttaa
     6361 tcgtactgag gcagagttaa attttttatt aagaccactt tcaaaaaact ttataatatt
     6421 tgaataatca attaatactt catctgcatc taatataagt aaccattcgc ccttagcgta
     6481 ggatatagat atatttctca tactagaaaa atcatcattc caattatgaa aatatacctt
     6541 ttctgtatat tgttttgcta ttcttatagt attatctgta gaacctgtat caactattat
     6601 aagttcactc tctattgaat ttctcaatac tgataacgat tttaaagttt tttcaagtat
     6661 attttcttcg tttttgacca tcataactat actaagtaac atatatattt tcctttcaaa
     6721 actatatttc taaaaaaaga aaagaataaa gcaaagcctt atcctttctt ttttattcaa
     6781 tatactaaat ttattctatc ctaataattg taaaactcct tgtggttgtt gattagcttg
     6841 agcaagcatt gattgtgatg cttgaactaa tatattcatt ttagataagt taaccatttc
     6901 tgaagcaaca tctgtatctc ttattcttga ttctgctgct gtaacattct ctaaagtatt
     6961 atttaaattg ttttgagttg attctagtct gttttgttgt gctcctaatt ttgctcttga
     7021 tgagtttaag ctcttaagag ctgcatctaa gctagatacc atagttttag ctgcatctgt
     7081 tcctgtagtt ccacctgcct tcatacttcc tattgaagca gttgttatac cagctgaagc
     7141 cattatactt gcagtattta ctaattgaat ttttatctca ttgttattat tacttgtacc
     7201 tgaaacatta gttccataac ttgctccaac ttgtaatctt attgttgaag aagttccatc
     7261 aagtagcttc ttaccgttaa actctgttga acttgatatt ctttcaatct catcttttaa
     7321 ttgtgttaac tcatcagcta tcttttctct ttcttcagta ttattaattt catttgcaga
     7381 ttgtaatgat aaagttctca ttctttgtag tatatttcca gtttcttcta acgaaccttc
     7441 tgctgtttgt acaacagata taccatcttg aacgtttctt cctgcttggt ctaaaccttt
     7501 tatttgagct ctcattttct cagatattgc aagtccagca gcatcatcag cagctctctt
     7561 gattctaacc ccagaagata acttctccat agacttactt tgtccattta cgtttcttcc
     7621 catttgattg tttgctatca aagcacttac atttgtatta actctcatta ttttaccctc
     7681 cttagtatag ttgacatcca tgtcatttct acttttaact tttgtattaa ctatccgttt
     7741 atattatcgg acaagttaat ctataacttt atactttttt attaaaaata tttacttttt
     7801 ctctatttga actataatac attgtgttta cctgtttagt aagcttgtac tcatgtaatt
     7861 ctttttttac ttctgataat tgttcttgca aagcatctct tatcttagca tcaatttcaa
     7921 atagctccat actatcataa agttgtataa attcactttt atcaacttca cttatactat
     7981 ttataatgtt ttgtctctca tctaattgaa aacttatatt tttatactcc attttttcaa
     8041 taaaatttat aatttttaaa gatatatctt tatataaatt tattttttct tctaacttgt
     8101 ccataattaa ttaccttgtg cttgtgagaa atagttcatt tgtgaagaat atttattcaa
     8161 caaagactct aaagctgaat atttagcata aagtcttttc tctttatcat tcattttttc
     8221 aactaaccta cttatatttc tttcttgctc tgcaatttgt tttgaatata cattttgact
     8281 agcagacgaa gaattttcca tacctgcttt tttagtaagt ttagaagctg aacttccaga
     8341 atattcaaat acaatttttt ttaatttttc aaacataccc ttatcatcat aatttgaaga
     8401 atatccagct aaagctttat ataccttgtc actatcatct ataagagcct tcgaaaaagt
     8461 atctgcatta aatgatattt gtcctttttt gttataattc tcatgctcac taatacccag
     8521 ttttcttaaa gcaataatag tatctgcatc tccaaaaaac atagactgaa tatcttcaac
     8581 aaaagctctt aattcatcat catttctaag tattccttcc tttgccttct tttcccactt
     8641 ttctatttct tcagttgtca tatcatcttt ttgttcatct gtaagaggtg gataatcttt
     8701 tgatttttta gtagtaacta atccataaac cttatccatc aatgcattat aatcatctac
     8761 aaatgctttc atatttttag tagcttcttc agtgtcagta acagaaacca gtttagcact
     8821 tccttttgaa tttacattat aatcaatgtt atctatttta aatgaattct gttctttact
     8881 tatgtgtgtt actcttcctt ggtcatcagt tacatcagct tctaaatttt ttccatatac
     8941 tactgcagat ttagttccac tagcatcagt tgtaaatgta ataccattat cactggcaat
     9001 ttgtccagct aaactatcat ttccaattac ttttaaatca atatctgatg atgaaccagt
     9061 ctgttttctt gagataatca attcacctgt catctcacta taagaagctc ttatatcatt
     9121 atctgcacaa agggagttta tcttacttac aatagtactt gtagtatctt gttcagatat
     9181 tggtatattt acaccaccta tatttaaaga acttgctcca ctagcatttg gaggaaactg
     9241 ttgaacaata gggtcacttg aatttaactt aatagttact tttgctggtt cagccatttg
     9301 agaaactgca aattgataat ttattttttc tgcaccagct gagccttttg ctactataat
     9361 actttcatca gaactagtta ttcttgtagc agaatatgct tttttatttg ttatactatt
     9421 aggagagtct ccatttaaat acttgtcata aagattcttt gactctttta taatatccct
     9481 gtatatttct tgtttccatt taactatttg ttgatcttgt ttcgctttat taactttttc
     9541 cttatctctt atcatactag cttctattat accttccata tcaaaatttc ctgaaaggcc
     9601 tgtaactctt actggactta tacttgacat atgtttatcc ccccatcatg attatagtta
     9661 tagtttatta tacacttcat gccatgtatc tcttatttgt tctactattg gcaatatatc
     9721 atcaattatt ttaatatctt tatttatatc agcctttact aattcatgtt ttatgaattc
     9781 ataaacttta tacatatcct ttgcccattg tcctacactt tggtctaatg tgaccattaa
     9841 ttctgtaaat atattttgaa ctctaacaag ttcattgtgt gcttttaaat agtctttatt
     9901 taaaagtgca actttagata tattagtata cttgactgta ccatctacaa gcattaatag
     9961 taattcttct tttgataacc catttagttc atttaaatct aactccatca tttataccta
    10021 cttctttctt ctctactttt tctatcagcc tcatgccatg tatctttaat ttcttctatt
    10081 acaggtaata cattatctat tatttgtaca tcttttctga tatttgccct tgaaagttca
    10141 tatcttacaa aatcataaac tctatacata tctttcgccc attgacctgc attttggtct
    10201 aatgtaacca ttagttctgt aaatatatct tgaactctta taagctctct atgtgctttt
    10261 cgtgtatttt tatctataat agctcctcta gctatttttg tatattttac tgcaccatca
    10321 acaagcataa gcaataattg ttcttttgac gccataaaaa tagcattttg cttataagaa
    10381 ttataaggat tttctccgta catatattac ttcctccaag tgttttattt taatgatttt
    10441 aagattttta tatttttatt tgtggctttt atattttcat tttttacttt ttcatatatt
    10501 tcttttctta agatacttat attattagga gcagatatag ctaattttat gttatttcca
    10561 tctactccta caacttttac ttctatgtta tctcctataa gtactgcttc atcttttttt
    10621 ctcgaaatta ctagcatcca ccatcacctc tcaataatgg atgccatatt gaatattcct
    10681 catcctgtaa aattatttgc attccgcaat tatttcttac atttattact ataggagctt
    10741 tcatgttcac agttgattct tcaagtgttt gaccaacagt gattatatta agcaacataa
    10801 tatcatttgg agattttact tcaagttttt ctattgtttc ttgatttagg actatttcat
    10861 attccttaat aatttcaaat ggtgatacaa caataaatcc tatacaatct tcttcagtag
    10921 aattaaattc ttttaaaata tcttcatttt ctatatctaa aagctcatac tcttttaagt
    10981 tttcaaatcc tagtattcct ttttttaatg taatcttcat catatctgcg ttattttact
    11041 atatataatc taatatagat acattaaata actttcctcc tgtttgtagt gaagcttgat
    11101 aagttaattc tgcagccttt aattgaacaa gcttttcccc ataatctatt tcatcatttt
    11161 gcgataaaac atcctttaac tgtatcaaac cttcatcatt tttttctttc atggcatcta
    11221 ctgtgtttgc ctttgtacca tatatagacc taacatttac tgtattatct attagattct
    11281 ccatgtcacc taaacaagtt gttgtcaact cttttatatt tgcatcactt ggatttgcca
    11341 gtttcttcga tatattattt aacttttcaa atggatcagt ttttgttgga tcaaacccaa
    11401 gactactcaa cttaacatta taatctacac tgattccttg agctacatct acacttaaag
    11461 aatcatttaa tttattaaga tttaagctag tattaaactt taaacttact gagccatcag
    11521 cattctctac acactcaaca ggtggtgtgc ctgtatttga acctgaaaat atgtacttac
    11581 cttcatgtgt tgcatttaaa gcatttgcta tttctttcat tttttcattg gttgaattac
    11641 taatagtttt tatttcgtca tctgaatatg aaccattccc acttgatacc aaacccttct
    11701 ttatatctgc taataaatta ccaagttggt ctaatgaagt atcagttgtt tctaaccaat
    11761 ctgctgtatc tttacaattt tcaatataag tttccatagt ttttatttca ctttttgaat
    11821 tcaatatctt tatcgcctta tatggattat ctgatagttt atttatttct ttcgttgtgt
    11881 tgatttgtct attgaccttg tctaatcttt gcaaattatc ttgcaatgaa tttagataac
    11941 tactggacat catacctgta ctaactctca ttataaattc ctccttttat taaattatcc
    12001 catttattac aacatccaat agttgtactg ctgttgacat aacttttgca ctagcttgat
    12061 aggaacgttg tactttcatg agttctgtca tttcttcatc taaagataca cctgaaacac
    12121 tcattcgtga attatcaaca ttatttataa ttttacgttg attcacttca tcttgattaa
    12181 ctttttgaga atctaaacct accgattctg caaaagcctt gtagtggtca cttattgtgc
    12241 ttacttttcc accagctacc ccaatatcta ttttttcatc ctttaactta gcaatctttg
    12301 aagcttcttc agtagtaagc ttcaattcat ttgaattact ttttattgct ggatttactt
    12361 ttataatatc ttcaccagct acatttgagg ttataaaaaa atctcttaca gttattccag
    12421 cagatgaatt ataaacatca tttatctgtt ttgctaatcc atcagataca tcttttaagt
    12481 cgcttttata tgtatcaatt ttaccgtgca tttcaaacaa gccttgtaaa gttcctgata
    12541 tacctgtttt ccaagttcca tctgcatttt tcaactcatt aagttccaat ttaccatctg
    12601 aaagcatatt tttaacatca ctattttcaa ggtctaactt aaaactcaag ttatccaata
    12661 ttctatctct ttcatcaagc aaatcatttg gtgtagagcc agaaccagag ataatatcaa
    12721 tagatttatt taagtcttca agacttttta acatgtcatt tatttgtttt atttcagtat
    12781 ctatattaga atctatatct ccttttaact tgtcaagctt cttataactt tcgcttatta
    12841 aagtcgctaa attagatgca ttttctacca ctagactttt tttactatca ctatttatat
    12901 cctttgataa tgactcccat cctttaaaaa aatcatctat acctgcagat attccatagt
    12961 ctgatggttc atttagtata gtttctatag tattataata atcatatttt gaaactgtat
    13021 tcccatactt atgagcttct gccctaaatt ggtaatcata aaaactattt ctagctcttg
    13081 ttacatctgt tacatggact ccagtaccta attgtcctct tccaattgaa ttgctcaaac
    13141 taggcatata cattggacta tttgtagcaa attcggtcct ttgcctagaa taaccatctg
    13201 tatctaggtt acttatatta tgagaaattg tctgtattga tttttggtgc gcattcattc
    13261 ctgaattagc tgtatttaaa atggaaaata atcctgccat atttctcctc ccaatatcta
    13321 ttttctaatg tttccataag aattataagt tgtagtttgt ttatcaactg gttttatgaa
    13381 attaatcatt tttttcgtga aaataagttc tttctttatt agagaatcat ttgattcttg
    13441 ttgattttta gctaaagcaa gaatatgctt taagtctata tatatttctt ttaaatattc
    13501 atcattactg ttttcaacaa cctcacttat tgaaacatca tcccctaaaa gttgtcttct
    13561 ttttatttct atagatgcaa gttctctact aatatcttct agtttaggat tcaacctatt
    13621 taaattttct aagtcttttt caattatata atcaaattgt tcttttagta attccatcat
    13681 attactaatt atcttttttt cttcatatat tatgactttt aagtctgtaa tcattacttt
    13741 atcctcgcat ctcctctatc atttttttag caatttcttc tggtttaatt ttgtaagttc
    13801 cattttctaa tgcacttttt atttcttcta tttttttatt tctagccgct tcatcgctca
    13861 tatttgatac acttgacatt tcctttgcaa atttagatag ttctattttg tctgttttat
    13921 caactttttg aacttgttgt acctttttta catccgattt gttctttaga taattattta
    13981 ctacaatatt tgaacttaca cttttaatat tcataacctc acctccaaaa ttacatcctt
    14041 atattttaat tatcgctcaa agaagaaaaa tatttatacg attcaaatag attttaaatt
    14101 ttttataaaa ataccccttg attaaaccaa tatagaataa tctaagggga tattttttaa
    14161 agtattaatt tacgtctgaa tcttctattt tagataatct tacagataat gtttcatttg
    14221 taataatact ttctccataa gcaaacaatc tgtcatttgc atatatgtct aatttttcat
    14281 ctacattctt atttagtttt attatagaac cttcttttaa atctaatata ttttttattg
    14341 tttctgttat gtttcctata gaaacagcca catttacttt agcattaaaa acattttgat
    14401 tttcctctat aatcccagtc ttttcttcta ttaattgacc aaaattcaat tcatatatat
    14461 cttctttttt gctattcata aaactattct ccttatgtaa tcttatctat acatatctat
    14521 caataagcct tgtatcatat caacagtaga aacaatatct atattttctt tttcttcact
    14581 aagtagttta ctagtcatat cctcaagttt attatcaata atctttacat ttttataata
    14641 ttgatgctcc cagaaactac tacttttatc aagtgaatac atattatcta caacagattt
    14701 taggtattct tttactgcat ttttatatct actaatgtca ccatagtttt gtgtaagtac
    14761 aagcctctcc ccagcctttt ttacaccatc taaattcctt tttatatctt ctagtgaaga
    14821 agattgtttt acaaagttta agctgtcaga aaacttatta ttttcctttg tctttttaac
    14881 ttctatactt ctacttggac ttatatttag cttgtctata tttataccca tagtaaatcc
    14941 taccctctat aatatttcat aactttaggt atataatttt gtgtctcctt tggcatttta
    15001 tacaagtcag aaattgattc tacgcctcta cttctcattc taccaggtcc accattatat
    15061 gccatcaatc ccatttctat gctacctcca aacatatcta tatattcctt aatatgtcta
    15121 gtaccagcat ctatattttg ttctatgtta aaagggtttg ttactcccaa atctctgcaa
    15181 ttttctggca taagttgcat aagacccttg gcaccagcag aagatacagt atttgggtta
    15241 aaatctgatt caaccttaat tattgccttt ataagatttg catctacatt atatttcttt
    15301 gatgcttgct ctacagcatt ttcaattctc gactttacac ttttatcttt tgtatcaact
    15361 tcaataaact taccaactgt attatttgct acactatcaa gttcattaaa cagtttttca
    15421 gaattagatg ataaattata tccattagag gtttgttgat tattttgtga taatgctttc
    15481 aacaatgtca tcataatcat atcaaatcca gatgaattat tatatccaca gttgcattga
    15541 tttgtggtat tactaccaga taattgggtc aatgccagta tatttattag tgaacttatt
    15601 tcgttcaaaa taaaactccc ctttctaaat tttataaact ttaaagttat ccttatataa
    15661 ttcaagatac ttgagttcct ataaaatctt cttgttatag actactttgt taaattaatt
    15721 tcttttatat tcattaaaac tttagattta gtttcactac attatgatta tcggataaat
    15781 aaaaaaaata tttatactaa aattatttac taattatttt tttaattgac taccataccc
    15841 ttttattaat actttccttt attatgatta ctattaataa ataatcaact ttaattaatt
    15901 ataacacagt atattttaat ttaagctgaa ctattatttc ttatataaat aaaaaagaga
    15961 tgtaatatta aatgattatc atcacattat tacatctctt caaatctaaa tatctaaaat
    16021 atatattcta gctatcaatt aaacatcaaa gttttatcta aaatacttaa ctccagattc
    16081 aaatatcttc tgttcttttt cccctatgat attcttatat acttcctcac caattctttc
    16141 agaatgtccc attttaccaa acactctacc atcagcacta gttatacctt caacagcata
    16201 gaatgaacca tttggattaa actcaatatc ataagtagct tcattattaa agtcaacata
    16261 ttgagttgct acttgtccat tagcaattaa ttctctcata acatcttcac tggcaacaaa
    16321 cttaccttct ccatgagaaa ttgctatatt atgaatatct ccaacattag tttgagctaa
    16381 ccaaggagat ttattagatg aaattctagt tctagcaatc ttagcttgat ggcgaccaat
    16441 attattgtaa gtaagtgttg gagcactttc acttggaact cttatttctc catatggaac
    16501 taaccctaat ttaattaata cttggaatcc attacagata cctaacatta gtccatcttt
    16561 ttgagttaaa aattcattta tagcttcttg cactctagga tttctaaata cagtagctat
    16621 aaatttacca gaaccatctg gctcatcacc agcactaaat ccacctggta acattattat
    16681 ttgagaagat tttatttcgt ttactattgt atctatagaa tcttctatgt ctttataagt
    16741 taaattctta aatactctta tacttgcatt agctcctgct ctttcaaatg ctcttgcaga
    16801 gtcatattca cagtttgttc cagggaatgc tggtataaat actcttggag tagctatact
    16861 taatgatgat tttttagctt ctccttgtga tatataactt atagtttcta tctttgattt
    16921 tacttcttct gttttagttg ggaaaatagg ctctaaagta ctacaatgtg ctttatataa
    16981 ttcatctaat gcaagttctt cacctttgat aaatatacta gcttctttaa ctgtacttcc
    17041 aagcacaaca taattgtatc cttctaacat acttaaatca tcatttgcta attcaagtac
    17101 tatatttcca taactagcct caaataattt atcatctgta aatgcttttt cagcctcttc
    17161 actaaactta aatcctattt tattaccaaa agccatttta cttattgctt cacctacacc
    17221 tgcaaatcct aatgcatatg ttgataaaac tttatttcca tgtataagtt ctcttacttt
    17281 atctaagttt ctctttaatt cctcaaaatc tacaacatca ttttctgcct tgtttacaca
    17341 taacattact acttttgagt ctgctttttt aaattctgga gatactactt tcttcgcatc
    17401 tactgtgtca acagcaaatg aaactaatgt tggtggaact tcaatatctt taaatgtacc
    17461 agacatactg tcttttccac ctatagcagg tatctcaaat ttactttgtg cataaaatgc
    17521 tcctaaaagt gctgagaatg gtttacccca tttttctgga ttgttaccaa gtttttcaaa
    17581 atactcttgg aatgtaagtc ttgttgtact ataatttcct ccaatagcaa ccaatttaca
    17641 cacagattct actactgcat acaaggcacc atggaatggg ctccattttc ctacctttgg
    17701 attgtatcca taagtcatta ttgtagaagt attagtctct cctccaagta ctggtatctt
    17761 agcaaccata ccttgtgttg gagtcgcttg atactttcct ccaaatggca taagtactgt
    17821 attaccacca attgtattat caaacatctc tactaatcct ttttgagaac aaacattaag
    17881 gtcagatagt acttttgtaa acttgtcttt catacttgca cacttaacat cttttactat
    17941 ttcattagag ctaaagaatg tattttcttc atctacttta gtaacatgaa catcagtagt
    18001 ttgttttact ccgtttgtat ctaaaaactc tctatttata tctacaattg cttttccatt
    18061 ccagaacatt cttaaatatc ctgtatctgt tactgtagct acatgcgtag cttctaagtt
    18121 ttcttctact gctaattgta taaatttatc tttattttct ttttttatgg ctactgccat
    18181 acgctcttgt gactctgaaa ttgcaagctc agttccatca agtccatcat attttttagg
    18241 aactaagtct aagtttatat ctaagctatc agcaatctct cctatagcaa cacaaactcc
    18301 acctgctcca aaatcattac atctttttat catttgagca acttctttat ttctaaagaa
    18361 tctttgtatt tttctttcgt taggagcatc tcctttttga acttctgcac tacaagttaa
    18421 gattgactct tcactatgtt ctttagatga accagttgct cctccacagc catctcttcc
    18481 tgtttttcct ccaagaagaa cgattacatc tcctgcttca ggtctttctc ttactacatt
    18541 ctcttttggt gctgctgcta taactgcacc tatttccatt ctctttgcaa caaagttttc
    18601 atcatatact tctgcaactt gcccagttgt aagacctatt tggttaccat atgaactata
    18661 tccatgagca gcttctgtag ttatttttct ttgcataagt tttccaggta aagtatcttc
    18721 taaagtcgtt cttgggtcag cacttcctgt aacacgcatt gcttgataaa cataacttct
    18781 tcctgataat gggtctctta tagctccacc taaacaagta gctgctccac caaatggttc
    18841 tatttcagtt ggatggttgt gagtttcatt tttaaacatt actagatatt tttctatctt
    18901 accatcaact tcaacatcta cgtttatact acatgcattt atttcttcac tttcatctag
    18961 gtcatttaat ttcccatttt tctttaattc ttttactgca acagtggcta tatccattaa
    19021 acatatatct ttatgtctgt tcacatatac attgtctctt gagttaagat acatttgata
    19081 cgcttctttt actatatcat taaacttacc atcttctatc tttacatttt ctatttcagt
    19141 cataaaagta gtatgtctac aatgatcaga ccaataagta tctaatactt ttatttctgt
    19201 tatagttgga tttctctttt ctgtatcttt aaagtatttt tgaacgtgtt gtaagtctcc
    19261 taaagtcatt gctaatcctt tttcacttac aaatgttttt aatccatttt catctaaatc
    19321 tataaatcca tctaatactt caactgttgt tggtatttca gtttccattt ttaacgtttc
    19381 tggtttttct aaagatgctt ctctactatc tactggattt atacagtaat cttttatttt
    19441 agaaaactct tcatcagtta ttttgcctga taatatataa acttttgctg tattaatagc
    19501 aggtcttatc ccttgattta caatttgtac acattgagct gcccaatcac ctctttggtc
    19561 gtattgacca ggaagaaatt ctattgcaaa tactctttca tcatttaatt ttggtatttc
    19621 ttcataatat accacatcta aatttggttc agagaatatt gtctttgctg cattttcata
    19681 aacttcttct gatattcctt ctacatcata tctatttaat attcttaaat tttctatatt
    19741 atctatgtgt aaactttcta ctaagtcttt ttttaaagct ttagcctcaa ggtcaaaacc
    19801 ttctcttttt tcaactaata ctcttctaac cattgagtct ttattttcag tattcaacat
    19861 accttatcct ccattttaga aaaattatct tcctatgtct gttctatagt gcattccttc
    19921 aaagtttatc ttctttatgt tttcataaac tttttctgct gcatccttaa ctgtagttga
    19981 ctttgcagtt ataccaatta ctcttcctcc attggtaacc aaattaccat caaacatctt
    20041 agtgccacta tggaatacta ctatatcatc atccaagttt tcaagaccag ttataatttt
    20101 ccctttttca taactgtctg gataccctcc tgatgtaagc ataacacata cagcttcatc
    20161 atcactatag tttatttcta tatcttttaa cttattatct agtatagctt ccattatctc
    20221 atgtaaatct gtttctaatc taaataaaac agattgtgtc tcagggtctc caaatcttac
    20281 attatattca agtacttttt ctccatcttc agttatcata agaccaacaa ataaaatacc
    20341 tttaaaatta agtccatctt ttttgaaacc ttctaaagtt ttttctaata ctgtctcttt
    20401 tactttattt gctaattctt ctgtatatat ttcactaggt gaaaaagttc ccattcctcc
    20461 agtgtttgga ccttgctcat agttgtttac ttttttatgg tcttttgcac ttgccatagg
    20521 aactattgta tcattgtcta caaatgctag aattgaagtt tctattcctt ttaaaaactc
    20581 ttcaattact attttatctc cagcagctcc aaacttctta tctgacatca tttcttttaa
    20641 agttgctata gcatcttctc tattttcagg tattacaaca ccttttccag cagctaatcc
    20701 gtccgctttt attactactg gatatccaaa actatctatt tctgatatag cttcttctaa
    20761 gtttgtatat tctttatatt ttgcagtagg tatgttatgt tttatcataa attcttttga
    20821 aaaagcttta cttccttcaa gttgagcaca ttttttatta ggtccaaata ctcttaatcc
    20881 ttctttttca aactcatcta ctattcccat aactagtggt acttctggtc ctactattgt
    20941 cagacctatt tcattttctt tggcaaattt taaaagttct tctatattag tatcacctat
    21001 atttacacat tctgctacgt ttgcaattcc tgcattacct ggtgcacaat aaattttttc
    21061 tacatttttt tctttgctta gtttccagca tatagcatgc tctcttccgc caccgcctac
    21121 aaccaatatc ttcataatat cctccttagt gtttgaagtg tcttattcct gtaaatacca
    21181 taaccatatc atacttgtca caagcttcta ttgaatcttt atcttttata gagccacctg
    21241 gttgaactac tgctgatatt ccatattcat gagctaatgt tacacaatca tcaaatggga
    21301 aaaatgcgtc tgacgctaaa acagctcctg taaaatcttt atctttgtta ttttcaagag
    21361 cattttttaa tgcccaaatt cttgatgttt gtccacaacc taaagctaga gtttgtccat
    21421 tttttactat tgcaattgca tttgatttca tattttttac tactctcata ccaaattcca
    21481 tgtccttaag ttgtgcatct gttggttttt cctttgttac attttcactt ttttctgcta
    21541 atatattgtt tctatcttgt atcaataatt ttccatctaa atatttcatt tcatatggtt
    21601 gtaaactatt ttctatttta gctaatttta atattcttag atttttcttt tgtttaagta
    21661 tctctaaagc ttctggttca aaatcatatg ctactactat ttctaagaat atttcattta
    21721 atttctctgc tgtagcttta tctatagtag aagttattcc tactattcct ccaaatattg
    21781 aaaccttatc cgcttcataa cattttgtat atgcttcaaa agcatctttt cctaaaccta
    21841 ccccacatga attagcatgt tttatagcta ctgaaactac ttcttcacta tctttaaatt
    21901 ctctcattac ttctaaacat ccatgtaaat catttaagtt gttaaaagaa agttctttac
    21961 cgcctaattg ctcataattt aagattggat tttttgcatt tgactgtgaa tataagaaac
    22021 cattttgatg tggattttct ccatatctta aagtttgttc tttttggaaa gttaaattta
    22081 atatgtcagg atacgtatct ccaacttctc cagcaaaata actagatatt aacgcatcat
    22141 atcttcctgt agttgagaaa gctttatatg ataatttttt tctgtcttct aatgtcattg
    22201 tatctgtttt taatttttct attatagaat cataatcttt tatatctact actactatta
    22261 catccttata gtttttggca gcagaacgta tcatagatgg tccaccaata tctatatttt
    22321 caattatttc atcatgactt tttccagctt ttaaagttcc ttcaaaatca tatagattta
    22381 cagctactaa atctattgaa tgtattttgt gttcatttac agtttctaca tgagattctt
    22441 tatctctttt ataaagtata ccaccatgta tatatggatt taatgttttt actcttccat
    22501 ctaatatttc tggaaaattt gtaacatctt caacttgcat tacattaaca ccattttctt
    22561 ttaatgtttt aaatgtattt cctgtagata taatttcata atctaattta tttaactctt
    22621 tagcaaattc cactacacca gtcttgtcag ttacacttat taaagctctc ttactcattt
    22681 ataaacactc tcctaccttg aagttttaat ttattttcac aaaaaagact tatactttct
    22741 tttaaaatcc tatgttcaac ttctaacact ctttttgcaa gtgtctttgc atcatcatct
    22801 tgatttacct taactacatc ttgcattatg attggtccag tatcagcacc ttcatcaaca
    22861 aaatgaactg tagcaccagt tactttagct ccgtaatcta taacaccttg atgaactttt
    22921 tctccataga atccagctcc acaaaatgaa ggaattaatg atggatgtat gtttatcatt
    22981 ttgtttctaa actcattaac cagctttggg ctgattattt ttaaataacc agccaaaact
    23041 actaaatcta ttttattttc cttaagaatt tctattatct tgtcttcatc agtttcacaa
    23101 attgctttta tgttatgatt tttagctctc tctatcccat aagcaccttg cttacttgag
    23161 ataactactt taacctgacc ttttatttct ccagactcag taccatctat aactgcttgt
    23221 aaatttgttc ctccaccaga tattaaaact cctatattta gcataattct acaccttcat
    23281 gactatctac tacttcacca attatataag ctttatcttc taataattca ctatattttt
    23341 tatctacata agcagcattg tcattttctc tattatttat aaagtttaca acatcatcag
    23401 cattttcttt atctactatt aaaactagcc caatacccat gttaaaactc ttatgaagtt
    23461 ctctctcatc aacagcatta aaaccttcta tcattttaaa tataggaggc ttctcccaag
    23521 attttttatt gatatctagt cctaaccctt taggtataac tcttgttata ttttctataa
    23581 gccctccacc agttatgtga gctatagctt ttatgtcatg tttagctaat acatctaaag
    23641 ctaatttaac atatatttta gttggtgtta aaagtgcttc tccaactgtc attcctagtt
    23701 cttcaacata ttgttccatt ttataattgt aagtttctaa aaatatcttt cttataaaag
    23761 aaaatccatt gctgtgtact ccacttgaag atataccaac caatacatct cctgatttaa
    23821 cattgtttcc agaaacaatc ttttctttgt ctgctattcc tacagaaaat cctgctaaat
    23881 catagtcatc ttctccatac attccaggca tttcagcagt ttctccacca attagtgcac
    23941 aaccagacat tttacaacca tctgcaattc caccaactat tttttcgata tgctcaggca
    24001 ctaatttccc aagagctata tagtcaagga agaataatgg ttttgctcct tgacaaatta
    24061 aatcattcac gcacattgca actaaatcta ttccaacagt atcatgcttg tccatcatct
    24121 gagctatttt taacttagtc ccaacaccat ctgtagatgc aagcaaaact ggttctttca
    24181 tacctacaaa atcttttaaa ctgtataatc cactgaagtt acctaaatct ccaactacat
    24241 ttccatcata agttcctttt attttacctt ttataaggtc tactgctcta tttccttcat
    24301 ctatatctac acctgattct ttatatgtta acatgaaagt agttcctcct tatcaatttt
    24361 cttaactggg tattctccat caaaacaagc tttacagaaa gtagacttat gttctgttgc
    24421 atctaacatt ccttctatat ctaggaattt taaactatca catccaatat attctctcat
    24481 ttcttctacg ttgttacttg atgcaattaa tttagagcga tttggagtat ctattccata
    24541 gtaacaagag taagctactg gaggagaagt tattctaaga tgaatttctt ttgctcctgc
    24601 ttctcttaaa gattttacta attgtttaga tgtagttcct cttacaattg agtcgtctac
    24661 taaaattata gattttcctt ttattattgt tgaaagagga tttaatttta tttttactgc
    24721 tatttctctt tcttcttgcg taggttttat aaatgttctt cctacatatc tatttttaac
    24781 taagccttca cttattttta atccactagc atttgcataa ccaatagctc ctggccaacc
    24841 tgaatctgga actggaacta ccatatctgc ctttacagtt tcattctgag ctaatctctc
    24901 accacattta attctaaatt catatgcatt tacattgtct atagttgcat catttcttgc
    24961 aaagtatatg tgttcaaata tacaactttt cttaactggt ttataatttt ctgaataaaa
    25021 atatgatttt agttctccat ctttgactac tattatctct cctggctcta catctcttat
    25081 aacttctccg ccaagtatat ctattgcaca attttctgat gcaaatatat attcatcacc
    25141 ctttttacca agtacaagag gtctgaatcc atgtgggtct ctaactgcaa ccaattcttc
    25201 ttgactcatt attacaagag catatgcacc ttttatataa tccattgtca cttttaaact
    25261 ttctactata tcccctttgt aatatctagc aagtatatat aaaatcactt ctgtatctga
    25321 attagtttgg aacatatatc catcttcttc tagcatatct tttaaataat ttgcattaac
    25381 tagatttcca ttatgagcta tggctaattg tctttttcta caacttccaa ctaaaggttg
    25441 acagttagaa acatgacttc ctccagctgt tgaatatcta acatgtccaa tccctatatt
    25501 tcctttcaac ttagataatt cattttcctt aaaaacatct ccaactaatc ccatatcttt
    25561 tttgtactta atttctccat catctaaaag agctaaacca cagctttctt gccccctgtg
    25621 ttgcatagaa tataaagaat aatatagttc tttagttaca tctttattag agtaaatccc
    25681 taaaactcca cacatacatt tgcctcctat ttattattca ttcttgaaag tacttccgta
    25741 taaccttcta caaggtctcc aaggtctttt ctaaatctgt ccttatctaa cttttcattt
    25801 gtatttacat cccaaagtct acaagtgtct ggtgatactt catctgccaa tattatattt
    25861 ccttcagaat ctttaccaaa ttctatttta aagtcaacta actttaggtt taattttaag
    25921 aaaaatgctt ttagtaactc gtttattttt aaagtttctt ctcttaagaa ttttaactct
    25981 tctctagttg ctaacttcat tgccactgca taatcatcat ttagcattgg gtcaccataa
    26041 gcatcatttt tgtagcttat ttcaaaaatt ggctcatcga aaactacacc ttcttcaaga
    26101 ccaactcttt tacagataga accagcagca atatttctta ctattacttc taatggtaaa
    26161 atctctactt ttttaactaa catttctcta tctgataaac tttttataaa gtgagtattt
    26221 atattatttt cttttaacat ttcaaagatt attgttgata tcttattatt tagtatacct
    26281 tttgaagata tttctgcttt tttttctcca ttaaatgctg ttgcatcatc tttataataa
    26341 acaacatatt cattttcatt atctgtagaa tatacttgtt ttgcttttcc ttcatataat
    26401 aacatttctg ctaccccctt aaattcttgt cgtcttctaa caccttttgt gccatctctt
    26461 ctctatatga tttcaaatcc tcttttaatt ttgggtattt taaagtcatt atttgtaatg
    26521 ccaataaagc tgcgtttaaa cctaagtcta ttgtaactgt tgcaactggg attccttttg
    26581 gcatttgaac tattgataaa agtgagtcta gtccatccat agttgatgat ttaataggta
    26641 atcctattac aggaattaat gtttgtgaag ctattacacc tggtaagtgt gctgcttttc
    26701 ctgctgctgc tattattaca tcagtatcat cttctatttc ttttaggaat attgaaagtt
    26761 gttctggtgt tctatgagct gataatgctc ttgctacaac ttctattcca tatttttcaa
    26821 gcagttttat cccttcttct aactttggat aatctgattt tgaacccata actactgcta
    26881 ctttcattag acttcccctc tccgtttata agttatttta cactacaaat cattcgtatt
    26941 attatacgaa ttatacacct taaattttat attaatatac atatattatc aactcttttt
    27001 tgaaattttt taaaaaaaat atttcttgta attttttcat tcgctacgta tcgaacttta
    27061 tttatgtttt tttgttaaaa taaaaaacag attatctcaa aattggtttt ctaattaaat
    27121 tttaagccca atactctttt ttaaatcttg tatattattt tttattactt ctttttta
//
LOCUS       DAFKMX010000033        26844 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFKMX010000033 DAFKMX010000000
VERSION     DAFKMX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.033"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            373..3471
                     /locus_tag="LZB08_001158"
     CDS             373..3471
                     /locus_tag="LZB08_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBM7314669.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3594..4880
                     /locus_tag="LZB08_001159"
     CDS             3594..4880
                     /locus_tag="LZB08_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBM7314670.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(5090..6196)
                     /locus_tag="LZB08_001160"
     CDS             complement(5090..6196)
                     /locus_tag="LZB08_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7314671.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(6231..7772)
                     /locus_tag="LZB08_001161"
     CDS             complement(6231..7772)
                     /locus_tag="LZB08_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBM7314672.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(8134..8880)
                     /locus_tag="LZB08_001162"
     CDS             complement(8134..8880)
                     /locus_tag="LZB08_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7314673.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     SKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(9092..10402)
                     /locus_tag="LZB08_001163"
     CDS             complement(9092..10402)
                     /locus_tag="LZB08_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7314674.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(10415..10885)
                     /locus_tag="LZB08_001164"
     CDS             complement(10415..10885)
                     /locus_tag="LZB08_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314675.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(10889..11740)
                     /locus_tag="LZB08_001165"
     CDS             complement(10889..11740)
                     /locus_tag="LZB08_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBM7314676.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            12633..13544
                     /locus_tag="LZB08_001166"
     CDS             12633..13544
                     /locus_tag="LZB08_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBM7314677.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            13719..14216
                     /locus_tag="LZB08_001167"
     CDS             13719..14216
                     /locus_tag="LZB08_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBM7314678.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPADKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            14960..15322
                     /locus_tag="LZB08_001168"
     CDS             14960..15322
                     /locus_tag="LZB08_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBM7314679.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLDVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            15387..16004
                     /locus_tag="LZB08_001169"
     CDS             15387..16004
                     /locus_tag="LZB08_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBM7314680.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            16001..16486
                     /locus_tag="LZB08_001170"
     CDS             16001..16486
                     /locus_tag="LZB08_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBM7314681.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTDTGELLVDSINKGKKK
                     L"
     gene            16529..17047
                     /locus_tag="LZB08_001171"
     CDS             16529..17047
                     /locus_tag="LZB08_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBM7314682.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            17183..18142
                     /locus_tag="LZB08_001172"
     CDS             17183..18142
                     /locus_tag="LZB08_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7314683.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            18168..19871
                     /locus_tag="LZB08_001173"
     CDS             18168..19871
                     /locus_tag="LZB08_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBM7314684.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            20084..22186
                     /locus_tag="LZB08_001174"
     CDS             20084..22186
                     /locus_tag="LZB08_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBM7314685.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            22199..22639
                     /locus_tag="LZB08_001175"
     CDS             22199..22639
                     /locus_tag="LZB08_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBM7314686.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSM"
     gene            22675..23478
                     /locus_tag="LZB08_001176"
     CDS             22675..23478
                     /locus_tag="LZB08_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBM7314687.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            23518..24114
                     /locus_tag="LZB08_001177"
     CDS             23518..24114
                     /locus_tag="LZB08_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBM7314688.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            24220..25083
                     /locus_tag="LZB08_001178"
     CDS             24220..25083
                     /locus_tag="LZB08_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBM7314689.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKYNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            25150..26139
                     /locus_tag="LZB08_001179"
     CDS             25150..26139
                     /locus_tag="LZB08_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBM7314690.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            26231..26842
                     /locus_tag="LZB08_001180"
     CDS             26231..26842
                     /locus_tag="LZB08_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7314691.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT        10493 a         3041 c         4556 g         8754 t
ORIGIN      
        1 atatcaaagt aacaatctaa ataatttatt attgaagaaa ttttaatata taatagtttt
       61 atatttttaa atgaatgaag tattatcctc atatatgatt ggaatactta attgaggtgg
      121 agatttgtca tttatatgag aattaattat aaacacataa tgagataatt aattataaat
      181 atattaaatt aagtattaca aaactctatt gtacgattga cacaataata attctattta
      241 ctaaaaaata gaacctcatt aaggtactat tttaaaacat aaagaagtat aatatcattg
      301 ccaaatatat agcaaaattt taaaagcttt aaaaattcaa tctggaaaat gaataaaagg
      361 agatatatat aaatggatgt aataaattta atagatgagt tggatgaaat tgtatatgtt
      421 tcttctttag atacatatga acttttatat attaatgaaa ttgggaaaaa ggcaatggga
      481 cttaatgata tatcccattt aaaatgttat aaagcattac aggggaaaga ttctccctgc
      541 gaattttgta caaactctat tttaaaagaa gatgaatatt acatatggga aaatacgaat
      601 actcgaacaa atagacattt tatattaaag gacaagttaa ttaaatggga gggcaaacat
      661 gcacgattag aaatagctct tgatattaca gaaaaggaag atataagtaa gtcaatagca
      721 gaaaaattag agataaagga gttattagtt ggatgtatta aacatcttat aacagaatct
      781 aatttttctg atgctgtaaa tcttgtatta accaatattg gtaaatttca taaagctgat
      841 agagcatatg tttttgaagt atcagaagat agaacaaaag taactaatac atatgaatgg
      901 tgcaatgaag gtgttgatga acaaaaacag gcactaacaa atcttgatat taatgaatta
      961 aagaattggt ttttattatt cgaacaaaaa ggttttgtaa taattgatga tatagaaaat
     1021 ataaaagaag aacagccaat agaatatgaa attttaaaac ctcaaaaaat tgaaagttta
     1081 atgacatctt cactaaaaca agatgataaa gtagtagggt ttattggagt tgataatcca
     1141 cataaagctg ttggagatac ctctcttttg acatccctat cttatttttt attaactgag
     1201 atgaaaaaaa gaaaagtaga aaataaatta aattatatga gctactatga cttcttgact
     1261 ggtgcatata atagaaataa atatattaag tatatagata attttcaaaa aggaaaagtt
     1321 aaatccatag gagttgtatt tgtagatata aatgggttaa aagatattaa tgataacttt
     1381 ggacatgcta atggcgattc tgctattgtg tcagcatgtg taataataaa aaaatacttt
     1441 cattcttata atgtttaccg tattggagga gatgagtttg ttgtattatg tgaagatata
     1501 tcaaaacaac tatttgaaga attagtgagt tggttaagtg aagaatttaa ggatgagatg
     1561 atatatagtg tagcagtggg cagtatttgg agtgacgata atattgatat acataagcta
     1621 attaaagttg ctgatgagag aatgtaccaa gataagaaga ggtattataa taagaacaaa
     1681 cttagaaata atgaaatgaa agtaattagt gacttagctg attcgacgaa taatatttta
     1741 tttaaacata aagagttagt tgataaggtt tattttgatg atttaatgat aaaagggtat
     1801 tctaaagctt atgaaaaatt agcttataat acatatgata taattttatc tatttgcatt
     1861 gatacgaatt taattactcc aatttatcaa tcagagagca atatttttag ttttcctata
     1921 aaggaaaatc ttgaaaaaac tttttcatac ataaaaaaac atttaattca tcctgatgat
     1981 ataatattat ttaatgataa ccaccttcta aaatctcttg atagtcttaa taatagcaag
     2041 caagacgatt tagaactaaa ttttgagtat agacgtttag gaatagataa aaagtactat
     2101 tgggcattaa tttatatgtt aaagattagt agtaaaaata agttaggtga gaaacaaaca
     2161 catattttag ttgctataaa aaatatagat tatcttatgc aactacataa aaaccaaaaa
     2221 gatacattaa caggactata caattatgaa agtttttgtg aacaaataag tttaatgctt
     2281 aaaagatatc ctgacaagcg ttatgcaatt ataatgatgg atatagataa gttcaaggta
     2341 attaatgaca tttatggagt tagaggagga gataaagctt taatatatgt tgcagagttg
     2401 attcaaaaaa acattggttc taatggtata tgttgtcgta tgtatgcaga tgtattttgc
     2461 attttttatg agagttactc tgatagagat attcataagg taatttataa gattaaaaat
     2521 agcttaaata gaaaaaaatt tgaatatatg ttagtaccat gttttggggt ttatagaatt
     2581 ttggataatc atattccaat tacaaatgcg tgtgaaatgg caggatatgc tcacaaaaaa
     2641 gtaaagaaac aaagtatcaa taactatata ttttatgatg atactataag aaatgatgca
     2701 cttgaagaaa aacagatgga aagggaaatg gaatacgcac ttgaaaatgg gcaattccat
     2761 gtatatttac aacctaagta taatcttaat gcaaataaaa ttgttggtgc agaagcattg
     2821 gttagatggg ttcatccaga aaaaggtata atacctccaa ttaaatttat ttcactattt
     2881 gagaagaatg ggtttattat cagattagat atgtatgtat gggaacaagt atgttctttg
     2941 attagaaaat ggatagattt aggtgaagag ccagttccta tctctgtaaa tgtatcaaga
     3001 ttacatttgc ataatccaca ttttaaagaa attgttttaa gtttaataaa taaatatagt
     3061 ataccaaaat cttttttaga attagagctt acagaaagtt tatttattaa aaatataaaa
     3121 ttatttacta agataatagc cgaccttaga atggatggta ttgttttaaa tatggatgac
     3181 tttggttcag catattcttc attaaatatg ttaaaaaata ttaatattga tacattaaag
     3241 atagattgtg gtttctttaa tgaggaagga atcacagaca gagaaaaaat agttttaaag
     3301 cacactatag ccatggcaaa agatttagat atggatgtag tggcagaagg tgtagagaca
     3361 aaagaacaag ctgaattttt agtgagttta gattgtgtgg taattcaagg ttattattat
     3421 tgtaagccta ttccaagtgc tgattttgag aagttacttt atcaaaaata agctaattat
     3481 tccattctaa atgtaggtag gatggagtag ttcaaaataa ttaaatagaa gtagaaaata
     3541 gtgaggatat aatttttaaa aagtataata tttatggatt aggagcgaag tttatgatag
     3601 tgggtattat aggtccttca gactctgggt ttaagataaa ggatgattta aaacagatag
     3661 acagtgattt aaagactaaa atatatgtaa gagaaaaagt agtagataca atagaagtta
     3721 tttctaaatg tgaagacgaa tgtgatgcta ttatatttac aggatgtgca gtttatgaat
     3781 ttataaaatc taagtatgag ataagtaagc cacattcatt tgtaccgaga agtgggacga
     3841 gtataatgaa ggcattttgg gcaataaaaa gtgcaaatat aaaattagat aaattcagta
     3901 tagatgtagt tgatagatat gttgtggaag atgcacttaa agaatttgat attaaagcaa
     3961 cgagtgtatt ttgtaatcca ttttctttgg aagtaggaga gtctgaactt gtagaatggc
     4021 atataaaatt atttgaagaa aataagacgg atataatgtt aactggtttt ggtgcagtat
     4081 ataatgaatt aaaagaaaga ggctatcctg tatttcgttt acaagctact atacaattaa
     4141 tcaaagaaag ttatgacaag gtaaagagtg aatatgctct aagtaaggca agattttctc
     4201 aaattgctgt tgaaatattg agtttaatag attataaaga gaagatagat aactattatt
     4261 ctgacatgat aaaaaaatca gatatggata agcttgtagt aaactatgta agaagcattc
     4321 aaggttcctt atttttactt ggaagaaatg attatgttgt atttgttcat aaaggggtag
     4381 ctgataatga gtataattat gataagctat tcaatctaaa gagagagata aaagacatgg
     4441 gcttttctct tagtatagga attggtactg gagtaactgc gtatcaggct gaaaataatg
     4501 cacataaagc attaagacac tccatagatt caaaagaaat tggtatatat ttggtagatg
     4561 aagatgaaaa tataagagga cctttagctt ccgaaaatga attaaattat tctttgatgc
     4621 tatctgatga aaaggtaatc gaaatatcta aaaagactgg aatgagttgt gagtcagttg
     4681 caaaaattat tgctataaat gagaatagaa aaagtaagat ttatgattca aaagagttag
     4741 cagagtatct aaatgtaagt gaaagaagtg caagacgtat attaaataaa atagtaaatg
     4801 caggtttagg tcgaatatgt gcaaaagaga ctagtattgg tggaggaaga ccaaaaaata
     4861 ttactgaaat actgttttaa gatattttat aaaactgtat aaatcattat attgtataaa
     4921 attctacaac aaattactat ataaagtatt aaagcttatt aggaaaaaaa ttagaacaaa
     4981 ttattataaa aatactaaaa cctatgtaaa acaactctaa tatataactt ataaagttga
     5041 agatacatag gtttttattt ttataattta aagagttcta ttatgatatt tataatttaa
     5101 tcatatgata aactattttg gcagtatcat ataattcgtc tatatttaca tattcattaa
     5161 ctgtgtgtac atcatacata cctagactta atatagcaca attatatcca agattagcta
     5221 atatatttgc gtcacttcca ccaccaatta ccattggatt tggaacaaca ccaacttgtc
     5281 ttattgcttc ttcacttaac ttgaaaacat gactatctct actaagttca aaactagggt
     5341 aagacatatt atgttcaaaa gtataagtag tattaaattt agatgcagca tccttacaac
     5401 atttttccat atgattaagc tcatactcta aagtttcagg tatatgagac ctaatttcag
     5461 cagtcaatgt aaccttatca gttactatat ttgtagctcc accaccttct attttaccta
     5521 tatttgaagt agtcaatgaa tctattctgc ctatgtgcat gttgcttatt gcatgagagg
     5581 ctactaatat agcatttaac cctttttcag gttctatacc agcatgtgct ttctttccat
     5641 gaaaagagat ttttatagat tgttgagcag gagctttgta tgctatcgaa ccaatagctc
     5701 caccagaatc taaaatcacc atatctttac aaggcaagtt gtcaggatta aagtttttga
     5761 caccatgcat accagcttcc tcacagattg taaaacaaag ataaatatct ctatgtggta
     5821 ttttttctgt aataacatgt tctaaagctt caagtatagc agctattcca gctttatcat
     5881 ctgcaccaag tgtagtagtt ccatcacttc taactacatt accatctagt attggtttta
     5941 cacctaaaca aggttgaact tggtccatgt gtgcacataa acaaagtggt ctacttcctt
     6001 cttcaccttt tatataggca atgacattac ctgtattacc accatatttt ttacctgcat
     6061 catctataat ggcatctata tttctttctt ttaagtagtt taccaaccac ttagacattt
     6121 ctaattcttg attagatggg ctatcaattc taaccatatc cataaaatta tttataagtc
     6181 tatttttatc taacataaat tatttctcct tttagaaaga tttaaataaa ttacatgcta
     6241 tatgaaggaa gtattccagg tcctagtgga atattaaata ccataaacaa tattaatagt
     6301 atagtccaca ctattaagaa tgatatagag tatggtatca tattagcaat tacagtaccc
     6361 ataccagcat ctttatcgta ttttcttgtg aatgctagta taatagggaa gtacggaaat
     6421 aatgggctaa gtggatttgt tatacaatca cctatacgat aagccatctg agtaagtgct
     6481 ggattgtatc caaccatcat aaacattggt acaaatatag gtgcaagaat agcccacttt
     6541 gcagatgcac tacctataaa taaatttata aaacatgata atataataaa tcctatcaac
     6601 aataaaggtc catttattcc tgctgatttt aaaaattctc cgccctttac agctagtatc
     6661 agacctaaat tactatttgt gaataattgc aaaaattgtg aagcagcaaa tgctagtatt
     6721 atatatccac ccatatcact cattgcacta gccattatag agactacatc cttatccttc
     6781 tttattttac ctatagtttt tccgtataca acaccaggta tgaaaaatgc tagtgctatt
     6841 ataggtacca tacctttcat aagaggagca ttgtttgata gtaaatctcc tgtttctggg
     6901 tcagctaaga atggtttttt accgattaca ctaagagcta ctataatagc cacatatatt
     6961 aatatactta taccagcttt tttaagccct ttcttttcta catcactaag acttccatca
     7021 acatctagtt ctgcatttcc ttcatattta ccaaatctag gagctacaat tttttctgtt
     7081 acccatgttg ccaaaagtat tagaacaaaa gttgatgcta tcataaaaaa taagttcatt
     7141 gttgcattgg cttgaaatcc agaatcaatc atttgagctg ctggaactgt aaaacttgca
     7201 gcaagtatat cattgacact tatcataatg tttgcggcaa atcctagaca gactgctgca
     7261 tatccagaaa ataaacctat aagtggatgt ctaccaacac caagatatac aagtgcagct
     7321 aatggtggaa ataatattgt acctgcatct gaagctatat tggcaagcat acctatagtc
     7381 attacaacaa gagttactct tgtttcagga atcttgctta ctgatgcttt gattgttgct
     7441 gtcataagtc ctgttttgtc acatacacca gcacctatca tagttactag aactagaccc
     7501 aaaggagcaa acccttggaa attgctgact atgtttccta aaagaatttg aagctgttct
     7561 acagtcaata aatttacagc ggttatagtt tctccagtac caggatgaac tactgatata
     7621 cccatagaac caactagact agatagtata agtaaaagta cacataaccc taaaaatatt
     7681 gtaactgggt ctggtagttt attaccaaca gtttctattc tgtttaatgt cctgtcaaaa
     7741 aagtttttct tcttaactac gttatcattc ataatatcct ccctaatgtt taaaaatata
     7801 attaaaaaat aagtattgtg gttaatctta caatttaaat tctgcttgtc gatttaagac
     7861 gtgtccacaa tatgtcctaa aaaaagtata aaataaactt tttcctgtgt caatgcagtt
     7921 ttctgaattt tctggttgag tggtgttagt taaatatgct ttaagtagat taatgacgaa
     7981 ttttatctaa tgtatttaaa caacatatgc gctttaaaat taattttaaa acaaaatttt
     8041 atgaaaagct agtggaagat taagttatta aaataagtag cgtagtaaag tgttagattt
     8101 tttaattaat caaataaatt tactttgtat tttctacaaa aatctgtgaa tatcttttga
     8161 attatgtaga ggatataaat ttattttatt tacatagatt agattttgtt ctaatttact
     8221 gtattcaaat ggttttccat gtccaggata aatcatttta ggttttgtat tgataattgt
     8281 tttccaagat tgaataaatt ctagtttgct ttctgcaaat atggtaattt tgtgagtact
     8341 tggaaatcca ttcattgcag aatctccaca aaataaaaca ccatcatcaa ttagtagtga
     8401 aattgaatca gcagtatgtc ctggtgtatc tataattttt ccatttagtt ctttttcaat
     8461 ttctttacga ttcttgtcag acacaagaat acaccttttt tcaaattctt gtttaagtgg
     8521 agaaaaacta tgctcacctt ttccaaatag tttcataatt tggcaaaaga gaaaggcagt
     8581 acggctggta caaccaccaa taaaagagtt ttgaccttta tatagaattt ctaaagcttt
     8641 atcatgcata acaatctttg tattaggaga ttcgtctaga atctgattta gaaaaccaac
     8701 atggtcatca tgagcatgag ttagaaatat gtatcgaata tctttgcttt ttatgttgaa
     8761 tttagaaatt cgttttttga aatgtttaaa accattttca tatccagtat caatcaagac
     8821 aaagccatct tgaatagggt acaaccatgt atttataatt cgatttcctg cattaaacat
     8881 tttttcacca ccttgaatta gaatataaat ttatattaat tagaaagaat atagcatagt
     8941 tctactgata tttcaacgta tctctaaaag ttttaaggag gataatatat tttagtttaa
     9001 tctaaaaact aaaagaaaag aagtatactg ttacaaaaac aaagagtggt ataactttta
     9061 cagtagatac caccctatta taaaaataga cttataaagt tttatcgtgt ttttctaaga
     9121 atgttagata ttcttcttta gtcataactg gagtaaaggt atctaatata tctttagcat
     9181 ccttagcatt atcaaaaaga aggtctacaa ctgtaagtgc cattgcttta gcaggaatta
     9241 tgtaagctaa atcttcatca actatttcaa aatctcttgt atgaagagct ccttttacac
     9301 caccaatcat agggtgaaga gtaggcatta tatgagacac atctccaaaa tcaaaagagc
     9361 ctgtaaaatc tccaccatca ataacttttc cttttagacc taaatcttca agattgtttt
     9421 taaataaatt atcaagactt ttgtatcgta atattggaag atatccagga atttcagtta
     9481 tctcaacatc agctccaact gccattccac cagccatcaa agccttattt attctttcat
     9541 tagcatcaat cataccattt atagttctag ctctaacata tgattccata tgaacatcag
     9601 caggaacaac atttactata tctccacctt ttgtgatgat agggtggaat ctaactcttt
     9661 cagactcttt aaaagtttct ctaagtgcat tcacgttgtt tatagcaagc attgctgcat
     9721 ttaaagcatt tattccatca tgtggagcag aaccagcatg agattcttta cctataaatt
     9781 taaccttttt tccaacaaat ccattactct caggaccaac tagagcctta tcttctccta
     9841 aatcagaagc atgaaacatc atagatatgt ctatatcatc aaaataacct ctttttatta
     9901 attcttgttt tccaccaaaa taagttattt ttccttcttt ttttaattgc tctctgtatt
     9961 ccaactcaat aaactcttca gcaggagtag ccataaaaga tactttacca tctaaatgtt
    10021 ctaaaacgcc actttttact aaaccaacag cagcacctaa cattccagct atttgaatat
    10081 tatgtccaca agtatgagaa gcacctattt cattagcatc tttatgttct ttacatgata
    10141 taccatccaa ttctccaagt atagatatat gaggaccttt cttgctagaa tttgcatctg
    10201 ctttgcatcc tgtaactgct atattttttt ctacagataa atttaattct tgctcaaaaa
    10261 agtttgaaac agcttctgta gttttaaatt ctttatatcc atattctgga tttttgtata
    10321 ttccttgacc tatttttaat attttttctc tattttcatc tatagttttt attactagag
    10381 attttaattg ttctttattc ataataaatt cctcctatat tttaccttga atacttaaaa
    10441 ctatatgagc tatactcgca catgcaaaga atatggttgt aaatacaata agtgaaatta
    10501 taactatttt ccatgacatt tcttttaaag cttctatttt gtttccaaca gaaattcctg
    10561 caaaagctag tagtggagta gttatagcca taaagttaat attattagtg ttagttaaga
    10621 atacttcaga tgttggacta tatggcatac taagtaaaag gccgattata gttgcaaatc
    10681 caaaagctgg aaagattgat ttaggaaata aatcttttat aatcatagca gccatagcag
    10741 ctaatattag catcaccata ccaggaagag ctgtaactat agatgtacca acgctaattc
    10801 tttgtcctat aagaattata actccaataa cagacataac caataattga tatgaacttt
    10861 tctttaaatt taaattagca aacatttatt agcactctcc ttctgtagat ttaatttctt
    10921 tagttttttt atttctcgaa aattttggtt ctaaaatctt atacataaaa ttagtaaatg
    10981 gtaaggatac aaaaactaag aatataattc ctgttatact agacatcata ttgcttgttg
    11041 cagcataggc aagtattgta tctgccattt caggtggtac catggtagat aatgaaccag
    11101 cagctgctgt catcatactt ccactaccaa caccacaggc cattcccaaa gcatatgggt
    11161 gaagaccact ataaatagat attgaaccta gtacactaaa gtatatagta ccaagaactg
    11221 ttcccattaa atatgtacca agtacaccac taccttctgg tgagtttatc ccatattttt
    11281 ctgatataac tcctaaagaa ggttcacgac ttatacttgc agtagctcca acagcttctc
    11341 ttttcattcc aaacattaat gcaagaggta atgcaacgat tggtgcaagt atatgaccaa
    11401 attcttgagc gataaatgct ggtccagcct gaattatttt gtcaatgttt ggtccaactg
    11461 tagtaccata ttttatacca agcattacta gtgcaattcc aacaacttca ccagcaatat
    11521 ttatttcttt ttctccaacc acttttttaa gtgctttaat ttttttacct aataaatctg
    11581 gtgttattat aagtccaatt ataactgcat aaagcatagg gaacagtact atagaggctt
    11641 ttccaatagc tatagttctt tgccctataa attcacatat tactaccaag attaagcaag
    11701 ttaaaaatac ttgaataaaa tgttttttat taatttccat gatttcctcc ccaaaagaat
    11761 aaatatttta cttatctgaa atgagaaaat acgttttctt aagtataata cgttttccta
    11821 aataaaatga aaaaactatt tcaaataagt ttcagaaatg tcaataacat gtccttaatt
    11881 aaggataaaa caaaaattta acattgtcaa cgaaaaatat ctaaaatatt acatattttg
    11941 aatatattgt aatatagctt tattatgaaa aaattgttta aatattgtag gaataatagc
    12001 taaaaagctt taaaattagt aatataaaat tgaaaaataa taatatagag ttgagaaata
    12061 gtaaattgaa attttaaaat aaatgagtgg aagtagctta gtaaagtatg gaaatgaata
    12121 ttacaataga aaataaataa tatgtaaaga ctttaaatta attttaagta ttaagtacaa
    12181 atactaattt ttgatataga aattgaataa agtaaatgat aataatcaaa tgaattaaga
    12241 tgataataag tgaattgata tagaaataag taaattaagt aagacgataa taagtgaatt
    12301 gatatagaaa taagtaagtt aagtaagatg ataacaaatg agataataag tgaattgata
    12361 tagaaataag taagttaaat aagatgataa taagtgaaat tgatatagca ataagtaaat
    12421 taagtaagac gataacaaat gagatgataa taagtaaatt gatatagtaa taaataaatt
    12481 aagatgataa tatccaaagt gagaaagata atagaaagta aataaaatca tgtaagaata
    12541 atttagataa gattctaaga ttttgtttat ttaaaaatta gtatgattaa tttagattaa
    12601 ataaatttaa taattttagt aggtggtaat gaatgaaaat aaatagatta acagaaataa
    12661 tagtaattct attaaataaa aaattagtaa cagctaaaga gttggcagat agatttgaag
    12721 tttctacaag aaccatatat agagatatag aaactctgtc tatgtcggga gtaccagtat
    12781 atatgacgaa aggcaaagga ggagggatat ctttaataga agaatattct atagacaagg
    12841 ccatattgtc aaaaaaagat aaagagagtt taatagtggc actgaaaaca ttacaggcga
    12901 ctaagtatcc agaaataaat tcagtagtaa ataaaattgg ctcaatattt ggagagcaga
    12961 atttttcaaa ctggattgaa atagacttta ctgaatgggg aagtaatttt aatgaagatg
    13021 ataaatttac aaaaataaaa gaagctattt taaggcgtaa tactataaat tttaattatg
    13081 taaatagttt gtcaagccag acaaatagaa ctgtcgaacc actgaagctt atgtataaga
    13141 gtaagacttg gtacttgtat ggattttgta aattaaaaga tgattttagg atatttagaa
    13201 ttagtaggat aaggaattta tctataaagg atgaagtgtt ttcaagaaag ataatagaag
    13261 aagtatgttt gaatgattct aaagtaatta aggaaaatac tataacgttg aaattgaggt
    13321 ttaaagaaaa gatgcttttt agagtatttg atgattttaa taaggattta ataactaaaa
    13381 atgaagatga tacatatgat gtaattacag aatttcctat tggtgaatgg atttatggat
    13441 atattttatc atttggtgac aatgttgaag tacttgaacc taaagatgtt agggataatg
    13501 taataactag actaagggag ttgtctaaaa tttattcatt ataatataat gctagattaa
    13561 agattttatt aaatatataa tatcttgcat ttagtatata atatctcact gagtttaatt
    13621 atattaaaaa tatgacataa aggtgtcgta tattataaat tataattgaa ttagttcaaa
    13681 ataaaataga ttaataaaaa caaagtgagg tataaagtat gaattatgaa atagtagaga
    13741 taaatgagaa aatagttata ggaatatcga aggagactac aaataaagat ggacaagcgg
    13801 taaatgatat aggtgaattg tggaaaaaat ttatgggcaa agggatatat aatgctataa
    13861 agggtaaaaa gaatgacaag acaataggtc tatatacaga ttaccaaggt gattttacaa
    13921 gtccgtatag ctttgttgca tgttgtgaag taaatagtaa ttctgataaa gagaagaatt
    13981 tagaagaatt aaacactaat aatactgttg gagaaagtat tattagtaaa gttataccag
    14041 cagataagta tgcaaagttt gttatagtag gaggtcaaaa agaagtagga gatttttggt
    14101 ttaagttttg gcaaatggat tttgatagga cttatataag tgattttgaa gagtatcaat
    14161 gtaatacttt tgatacaaag aaacaagaag ttcatattta cataggaata aagtagattt
    14221 gttattacta tattgaatga agtattgagt atagtacatt tattatagat agttttatta
    14281 tttataacta taagcagtaa atctgtaaaa gatttactgc ttttttagta attaatagta
    14341 tcagtatcac tttgaatctt cagacatatt cttcatttaa tattgtttat tgataaatga
    14401 cacttgtaaa tataatgatt tgttatttaa tttaaaatat gaaaaaaagt attataaaca
    14461 cattagaata cttctggatt tatgggtata caatttgaaa aatataaaaa ataatgtata
    14521 aagctaaatc taatttaatc gataatacat atagagaaat ataattaagt aaataaacaa
    14581 ctatctatta gatattgaga aagtcaaatc ctatatgaat ctacagaaat gattttaatg
    14641 aatctgaaaa ttttttttat acaaacataa aaaaggtata aagttgtttt attataattt
    14701 aattactttt gataactttt tatgttttat acaattatat ttttaataac tcgtcaaaac
    14761 gccaaacata tattacaata gatatataag tttttttgtt gacaaattat gttaagagaa
    14821 ttaagtataa tatggcataa ataattactt ttatttatca atgaaatgta tttattgtgt
    14881 agcgaaaaat agtcagatag tatagacaat aactgaagat aaaagcgttt attgttgtac
    14941 atatggagga gttagtttta tggaaaataa aattttgata gtagatgatg ctatttttat
    15001 gagaatgata attaaagaag ctctgagtaa aagtggctat aataatttaa tagaagcaaa
    15061 tgatggagaa gaagcatgta atatatttaa atatgaaaag cctgatttga tgttgcttga
    15121 tattacaatg ccaaaaaaag atggtttaga tgtgctaaga gaaataaaaa agttggattc
    15181 aagtgctaag gttgtaatgt gctctgcaat aggacaagag aatatgattg tagaagccat
    15241 aaaacttgga gcgttagatt ttatagtgaa gcccttcaaa acagaaacat tagttaaagt
    15301 tgtagataat gctttaaaat aaaagtgtaa atatacacta atacaatgaa aagcaaataa
    15361 atgcaataat aaagggaggg gtttatatta taaattattt agaacttgat gatgttcata
    15421 tagatgctct tagagaaatt ggtaatatag gctctgggaa tgcaattact gcacttgcat
    15481 ccatgataaa tagcaatgta gaagtattaa tacctatggt caagatactt gaatataatg
    15541 aagcgacaaa tttgcttggt ggtccagaaa acaaagtagt atgtatttta ctcgatatga
    15601 aaggtgatat caatggaatg tttatgtttt tgttggatga atcaataact caacttatgc
    15661 taagttctct ttttaataaa gaagaagctt ttttagatga gattgaggca attgaaattt
    15721 cagcaattaa ggaaatagga aatattatgg caagttcata tgtaaatgct attgcatcta
    15781 tgttaaacat gactataagt gtttcaattc cagatatatg tattgatatg gttggagctg
    15841 ttctaaatgt acctatgata agattttcag atgttggtga caaagtctta tttatagaaa
    15901 ataaatttaa aatgagtgat aattatttta ctagtcacat tttgatgatt ccagaaatga
    15961 gctcacttag agaaatactc gtgaggttgg gattagaagt atgaatagag agatagtagt
    16021 gaatattgca gatatgaaga tagcctatag accaaatgtt ttagttactt atgcacttgg
    16081 ttcttgtgta ggagtatgtt tgatagataa agtagctgga attggtggaa tgttacacgt
    16141 tatgttgcca tatagtaaag atgctattaa cattgaaaat aagtgtaaat ttgctgacac
    16201 aggaataaat gagctcatta aatctatgga gaatataggt gcaaatagaa tatatataaa
    16261 agcaaaaatt gctggaggtg ctcagatgtt tttaggaagt agcaattcag taattgcaag
    16321 tataggacat aggaatgttg aggcagtaaa aaaaactctt ttaggattaa atattccaat
    16381 attagctgag gacacaggta tgaattatgg tagaacaata cgtttttaca ctgatacggg
    16441 tgaattactt gtagattcaa taaataaagg taagaagaaa ttataatata tatttcatag
    16501 aaagtgtatt ttaggaggga ggtattaaat gagatttcca gatgacccat atttagatta
    16561 tggactaatt gatgaaggaa ttaagtatct aaagtttaga gtagatgagc aagactttgg
    16621 ggttgaactt gaaaaggtaa tagaaattac tggaaatcaa gaaattatat ttattccaga
    16681 actacctaca tattctaaag gcataataaa tctaagagga aaaatcattc cgataataga
    16741 tatgcgtttg agacttaaaa aagatgaatt aatgactaca aattgcatgt atatagtagt
    16801 aactgaaata aaggacttgg tagttggatt tgtggttgat agagttgatg atatagtaag
    16861 cttagaaaaa agtaaaatat cacctccacc aagagttact atagaatatt cggattattt
    16921 tgtaagtgga gttggagaat tagaaggtaa aataattact ttactagatt tagagaaact
    16981 tttgacagat aaagatgaag ttttaataga tgaattaatc aatgggtttg attgcactat
    17041 catgtaacat agggatgtag ttagagtgtt attataaaaa gattgtgatg agaacattta
    17101 agaatatagt ggtataatta ttaagtataa aaattttaaa tcattaaaat ttgaatatat
    17161 tatgtttaag agaggtttgt atatgaaaaa taagcaacag ggaacaattt tagtcgttga
    17221 tgatagtgtt ctgatgtgtg acttaataga taaaacactt acaaaagata attttaaggt
    17281 taaaaaagca tttaatgcaa tagaggcaaa agaatttatt gaagaattta taccagacat
    17341 tattttgctt gatataattt taccagataa ctctggtttt gagttttgta aggaagtaaa
    17401 atctaatgct cgtacagcag atatacctat aatttttata acttcaaaaa atacagatac
    17461 agacatagta aaaggatttg gtgtaggagc aattgactat atggtgaaac cttttagtat
    17521 gacagaatta aaagctcgtg ttatagctca tcttgaagta aagaaatctc aagataaatt
    17581 aaaaaagata aataatgaac tggaatcttc tcttgaaaaa ttgaatagac tcgttgtaag
    17641 agattatctt acaggtcttt acaataggcg acatataatt aatcagctta tggagcaaag
    17701 aagaattaat aaatatagtg aaacatcaat atcccttatc cttggtgata ttgatgactt
    17761 taaggttatc aatgatactt atgggcatga agcaggggat ttcgtgttaa cagtaatatc
    17821 aagtataata aaaaagaatt gtagacaaat tgatactgta tcaagatggg gtggagaaga
    17881 gtttttgata atgctcacaa atactccaat agaaggtgct aagatacttt cagaaagaat
    17941 ccgtaaagag ataaaggagt ttgatttcaa ctataaagaa cataaaataa agtgcactat
    18001 tacattagga gtagtagaag taaactcaaa tatatcatta gaagaaaaca tatccttagc
    18061 agataaagca atgtatgagg gtaaaaactt aggaaaagat tgtagcgtag tgagtacaat
    18121 gaaaggatta aaaaagatat aaacttactt tatgaaggag tacagatatg aaaagagaag
    18181 taaagctttc taaattagca attgtattgt tgattattat agtattggct gttctagcag
    18241 ggattttttc ttctatttca tcttataaag catttgaaaa agtagaaaaa agtattaatg
    18301 atatgaataa tttgaggact caaagtcagt atataaaaga tacaactaga gagataagtg
    18361 atgtattaag aagatatgta tttacaggag aaaaaaagaa taaagatatt tatgaaaaag
    18421 aattatacgg aaaaacaaga gaatcagctt taaacaaatt agaaaaaatt ggtcttacaa
    18481 agagcgagtc aaataaaatc ttagaagcaa aagaaatatc agattcttta gtaccaacag
    18541 aacaaagtgc aataaaggca gtcgaaagta atgataagag taaagctcaa agtataattt
    18601 ttggtagtga ctatataaac tcccaagaga aaatggattc tttaataaat gaatttcaag
    18661 aggatatagt tacccgttca aattccaaaa ttgtagaggt aaaaaaagaa ttacaaagtc
    18721 taatattaag aacaatattt atactttccc ttttaataat attgacaata atagaatata
    18781 cagtaataaa attaaaaata gtgattccta ttaataaagt agaaaaacac ttttcaagaa
    18841 ttgcatctgg ggacttaaga acaagtatag acgttgaaga aagtaaatct gaaataggac
    18901 tattagtgag ttctgtcaaa aagatgcaaa gtatgctttt aaattacata gaccttatag
    18961 acagtacatt aactagtatt gcaaaaggag atttaagagt agagatagac caagaattta
    19021 taggtgattt taaatcaatt aaaatatctt tagaaagtat aataaactct tttaatggtg
    19081 atttttctga aataaattta tcagcagaac aagttgcaag tgcttcagaa caagttgcag
    19141 caggtgcaca agctctttca cagggtgcta ctgaacaagc tagttcagta gaagaattag
    19201 cttccacgat taatgagata tcagatggaa ttaagaagtg tgcagatagt gctagtcttg
    19261 catgtgaggt atctgaccaa tctgcatcac aagttggttt cggaagtaga tgtatggatg
    19321 atatgatgac atcaatgggt gaaataagtg aaaaatcgag tgaaatatca aaaataatca
    19381 aaacaataga tgagatagca ttccaaacta atattcttgc attaaatgca gctgttgaag
    19441 cagcaagagc aggtcaatat ggtagaggat ttgcagttgt agcagatgaa gttaggtctt
    19501 tggctggtaa aagtgcaaaa gcagctcaaa atacagctat tctcatagaa gaagctatta
    19561 aaacagtaga aaatggtgtc agaattgcta aagagactgc aaatgcattg gacttggtag
    19621 taaatggtgt agaaaaaact actaattata ttaatggtat ttcttcggct gtacatagtc
    19681 aagaagtagc aattacacag attttatctg gaatagagca aatttccata gtagtccaaa
    19741 tgaactcagc aacagcagag gaaagtgcag cagcctcaga agagatgtct gctcaagctc
    19801 aaattttaaa agaattagta gaaaaatttc atctaaagga aagtgaagta agagaatcat
    19861 catgggttta aattatgaga tgaatattga agtaagagaa ttatcatgta tttaaattat
    19921 gagatgtata tatattgaag taagagaatg attgtgtatt taaattatga ggtacatata
    19981 gtgagataag agaatcatca aggatttaaa ttatgagatg catatataat agaaaattat
    20041 ttgtaggtcg taggtgataa atgtgaatta tattgaaccg agtatgagac aaatgttaga
    20101 tatgtttata tttgagacaa gtttattact cgaacaatta gatgaaatta tgattcaaat
    20161 agaaaaagag aaagtattta ctcataacaa cattaatgaa acttttagaa taatgcatac
    20221 gataaaaggt tcagcatcta tgatggggtt agacaatata tctgctttag cacatagtgt
    20281 agaagacttg ttttatgcta tacgtgaagg taaaatagaa gttttagaca atgacctatt
    20341 atttgattta gtctttcaat cattagattt tataagaaga gatatagatg agttacaatc
    20401 aagaggttgt atatcttctg attactctaa ttttattgaa ggaattgaag agtatgtgag
    20461 tactttggaa ttatcaagtt ctatgaaatc aaaggataat gcaaatttag aaagtacaga
    20521 cgaaaaagaa gtaatagatt caaattttga atacaaagaa gactctgatg tagtaaaaat
    20581 aatctttgaa caaggttgca tgatggaaaa tgtgagagct ttcatgcttg tatttgaact
    20641 tagagattac tgtgaattta tagattttta tccaaaagat gtagaaacaa atgcagagtc
    20701 tacagagtat attaaaagtt atgggtttta tatacaattt gtagctaaag aggataaggc
    20761 aatagtttat aaaactatag aagataatct ctatgtaaaa gaatacataa atattagtaa
    20821 aaaagaaata gaaatgaaaa atacatcaaa caatgatgca gtagaaaata aattaaaaga
    20881 taataataaa gtaagtaaaa gttatcaaaa taactataca gaagatgaga tacaagaaga
    20941 tgttttaaat gaaaaaactg atatggaaaa agatagtcta agtgaagaaa atgaacattt
    21001 accaataagt aagcagagta ttatgagtgt aaatttagaa aaattggact tacttcaaaa
    21061 tatagttgga gaaattgtaa ttatggaatc aatggttgta aatagcgtaa attctacagg
    21121 tgttgatata gataatttca ataaggcaac aagacaatta cacaagctta cagatgaatt
    21181 gcaagatata gtaatgtcta tgcgtatgat tcaaattgct ggtgtatttc aaaaaatgca
    21241 tagaattgta cgagatatga ataaaaaatt agataaaaat gtagagttaa taactatagg
    21301 agcagagaca gaggtagata agagtgtaat tgataattta gcagaacctt ttatgcacct
    21361 tataagaaat gctatggacc atgcaataga gcctattgag gagcgtatag cgaaaggtaa
    21421 agaagaagtg ggaaagatag ttttatctgc tgaaaatcta ggcggagaag ctgtgataag
    21481 tatttcagat gatggtagag gcattatgag agataagatt ttggcaaaag ctaaggaaca
    21541 aggtcttctt aaacatcaaa gtaatgagta tactgatgac gaggtcaatt ctttgattat
    21601 gactccaggc ttttctacaa aagaatctat aactgaattt tctggaagag gagtaggcat
    21661 ggacattgtt cgtaaaagta ttgaaaaagt tggaggttca attgaagtaa aaagtaaaca
    21721 ggatgaaggg acaaccttta ctataaaaat accattaact ctttctatag tagagggtat
    21781 gaagtttaaa gttggagatt cactatttac tttaccaata tcatccataa gaagaacatt
    21841 taaacttaca gacttaaatc aaataattac agaaggaaat aagacagagt taattacaat
    21901 atatggagcc tgtattccca ttataaggtt acataaaata tataatattc aaacaaaagt
    21961 aaccaatctg atgaatggta atatgataat tattgaaaat gagcataaag cagtatgcat
    22021 ttttgttgat gaaataatag gtaatcagca agttgttgtg aaaccatttc cagtttattt
    22081 taaccgattt aatataaaaa ataagggttt atcgggatgt acaatacttg gggatggaag
    22141 tataagttta attatagatg cagatagcat tatagaaata tattagggga tttaaagtat
    22201 ggctaaagat gaaattatta aacaagtatt aacattttat gtcaatgatg ttatttatgg
    22261 aatagaatta gaaaatgtga ttgaaaccat aagatttcaa tcaataacgt atgtaccatg
    22321 tcttccaaca tatataagtg gagttattaa tttgagagga agaataatac cagttattaa
    22381 tatgcatata aagtacaatc ttcctaaagc tgattataat gagagaactt gtataataat
    22441 tacaaaggtt gatgagtatc aagttggaat tattgtagat aaagttgttg atgttattca
    22501 tatagaaaat ctaaatttac tagagacgac taattctaac aacactaata ttaataaaga
    22561 tataaaagag attgcgaaag ttgaagataa ttctatatta attttagata ttcgaaagtt
    22621 tttgattaat agcatgtagt tagttctgat aaattagcag gaggaagttt ttgcatgata
    22681 aaactcacag atgaagaatt tatagttctt gtaaaccatg taaaaaaaga atatggtatt
    22741 gatttaagta aaaaaagagc gttaattgaa ggtcgcttat acaatacaat gatagagaaa
    22801 aaacttagtt cttttagtca atatatgaat ctattattta aagataagac aggaaatgaa
    22861 gctataaacc taataaatag attgagtact aaccacacat tttttatgag agaacctcaa
    22921 cattttgagt ttatacagaa tagtatactt cctttttggg aagaaaataa taaaataaga
    22981 aacataaata tatggagtgc aggatgttct agtggagaag aagcgtattc aatagctatg
    23041 actttggatg attattttgg atataataaa gagttgtgga acattaaaat catagccaca
    23101 gatatttcaa ctaactcact tgaaaaagct aaaagaggta tatacataga atcaaatgtt
    23161 aacaatgttc ctgagttatg gaagaagaag tattttatag acaacaagga tggtacattt
    23221 aaaatttgtg ataagattag aaaaagtgta atttttaagc cgtttaattt aatgaatgat
    23281 ttctcatatc aacattttga tttgatcttt tgtagaaatg ttatgattta ttttgattta
    23341 aaaacaagag aagaacttat aaataaattt tataatgcta ccaaaaaaga tagttttttg
    23401 tttgtaggac atgcagaagt aatcaacaga agtagtacta aatacaaata tgtaaaacct
    23461 gctatatata aacgataaaa aggtattttt gatatatgat tttttggagg tggtaatgtg
    23521 gaacgtttta ataatatggt tattgctata ggcgcatctg taggtggtac agaggcaata
    23581 cttgaaatta taaaagacct accaaaatca acaccaggga ttgttgtggt tcaacatatg
    23641 ccagctatat ttacttatat gtatgcacaa cggcttgaca agcagtgtat tatgaatgta
    23701 agagaagcta agaataatga tagagtcgaa caaggaaatg tgttaattgc acctgggggt
    23761 taccagatga aattgtgtac ggataaacaa ggatattatg taacttgtga aaagggagaa
    23821 agaataagtg gtcactgtcc atcagtagac gttttgtttg attcagtagc agaagtagca
    23881 ggtaaaaatt caataggaat aatacttaca ggtatgggtt atgatggagc aagtggactt
    23941 ttaaaaatga aagaaaatgg ttcatttaca attggacagg atgaaaattc ttgtatagta
    24001 tatggaatgc caatggtagc ttttgataga ggttctgtta tgttacaatt acctcttggt
    24061 gaaatttcaa actgtttgat aagacatatt aatattttaa aacaaaaaga ttaatttttt
    24121 ataaataaaa attaatcttt tattaaatta tatggataaa aaaccctaga aactccaaat
    24181 ataataagag agatttatat ttaggaggaa aatgataata tgaaattttt taaagtcgat
    24241 acaaaagcta aagttttaag atatgtattt gttttaacat tgactataat ggctgttttg
    24301 gcaattaaac atgactataa atataataat ttaaggaaag atgcttcaat taatttaaat
    24361 agtaataagg aagcagtgac tgttatgagt aaccagtcat taccaaaggg taatacaaat
    24421 agtaaacaaa ctttatcagt aagtacaata ggaagtaaaa caatagtgaa aaatgatgag
    24481 tatttagaaa gtactataaa catgcctgta ataacaaatt caaataaaat tgttgaaagg
    24541 tctacaaatg ataagataaa aaatgacata tttgaatttt ataataaatc atataaagaa
    24601 gcaaagcagt atcttaagta caatcctgac gagaaaaata agtttgttgc aaatgtagac
    24661 tttgaactta aaaagaatac tgattctgct cttagtataa aagtgagata ttatacttat
    24721 agtggtggag cacatggatt ttatcaagat atagcatata atgtggatat gagaactgga
    24781 aaatttttag aattaatgga cttgtttaag gataatacta aatataaaga agttatagat
    24841 gaagaaatta aaagacaaat agctgaactt gaaaaaaagg atgaagaaaa tatcggaata
    24901 tataatttta aaggtataaa agagaatcaa aacttttact tgcaagatga gaatctagtt
    24961 gtatattttg atttgtatga tataactcca tatgcagcag gaataccaga attttcaata
    25021 aataaagcgt tgataagttc tatgctaaaa ccagagtatg tagatttatt tgatttgaaa
    25081 tagaagatta aattaaatgt tatgctataa tatttagcat agagacttat taaaattggg
    25141 ggtataaaaa tgctactttt aaaaaaagac gacataaaaa aagtatttac aatgagagat
    25201 gcaattgaag ctgataagga agcttttaga atttattgtg aaggaaagag tgtaaatcct
    25261 ttgagaacca atatatctgt tccaagtcaa gatgctagta tgttatttat gccaggatac
    25321 gtagaagaat taggctgtgg gggaataaaa attgtatctg tatttcctaa aaatgctcaa
    25381 aaaggcaaac cagtaattcc agcaacagta ctattattag atggagaaac tggagaagtt
    25441 agtgcagtat tggatggtac ttatgtaact cagattcgta ctggagcagc ctctggagca
    25501 gctatagatg tattggctaa aaaagattct aagattggag ctttgatagg tctaggagga
    25561 caaggtgagc cacaacttga agccatgata gaagctagaa atttggatga ggtaagagtt
    25621 tttagtagaa acaaggaaag tagagaaaaa tttgcagaag aaatgaactt aaaattaagt
    25681 aaatataata ctaaaatagt tgcagttgaa tcatctgatg aagccataga taacgcagat
    25741 gtgatagttt tagctactcc atcaaaacaa ccagtactta atggaaactt agtgaaaaaa
    25801 ggagctttaa taagtgcagt aggttcatat atgccagata tgcaagagtt gtgcccaaac
    25861 tgtttaacta gagcgtctaa gatatttttt gaatcaacag atgcagtttt atctgagtca
    25921 ggagatatat taattccatt aaaagaaggt aagataagta aagatgattt tagtggtgat
    25981 ttagggaatg ttataaacgg aactattcct ggaagagaag atgatgatga gataattgta
    26041 tttaaaactg ttgggatagg tgtacaagat gtcgtaacag caaaaagaat atacgataaa
    26101 gcaaaagaaa acggtattgg aatagactgg aagtggtaaa atattcaaaa taattctata
    26161 gttagacaaa tttatgttat aatgttgata aataaatttt ttattatttt aattgaggga
    26221 ggatgtttgt atgaaaatgg taaaaaagtt aatattatct acgattatgg ctctagtttt
    26281 agttctaact atagggtgtt ctaatgcaga agaaaaagta aagtcgaatt ttgaagctaa
    26341 tttagctgca ttagaaatgt ataagtatga tgaaaaagga atttctgatg cattaacaga
    26401 agctgaatat aaacagattc aaacttataa cagcaaggac aaggtttcaa tgattgctca
    26461 attgacacct ctttatggta aagaaaatgc tgaaaagata tacgaaacaa ttgttaaatt
    26521 aattcaaggt gttgaaaaga cagtaacagt ggaatcttct agtaaggata agataacttt
    26581 gaaagttgta agtaaagctt tagatactag taaagtaatg agtgaagcga acactttagt
    26641 aaaatcttat gtaactgatg aaaagaatta cgataaaaaa gaaaaagaag ttcaagatga
    26701 tatgacaaaa atgattatag acctttacaa taagaaacca actaaggaag ttacaactat
    26761 gattacatat actaaaaaag atggtaaatg gcaaccacct agtaatatac aaaagttatg
    26821 tgaaggaata tatacaattt aatt
//
LOCUS       DAFKMX010000034        26517 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFKMX010000034 DAFKMX010000000
VERSION     DAFKMX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.034"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(214..1293)
                     /locus_tag="LZB08_001181"
     CDS             complement(214..1293)
                     /locus_tag="LZB08_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314692.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      1581..1696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1871..3241
                     /locus_tag="LZB08_001182"
     CDS             1871..3241
                     /locus_tag="LZB08_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7314693.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(3506..4117)
                     /locus_tag="LZB08_001183"
     CDS             complement(3506..4117)
                     /locus_tag="LZB08_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBM7314694.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(4107..6470)
                     /gene="lon"
                     /locus_tag="LZB08_001184"
     CDS             complement(4107..6470)
                     /gene="lon"
                     /locus_tag="LZB08_001184"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBM7314695.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKESFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            6650..7162
                     /locus_tag="LZB08_001185"
     CDS             6650..7162
                     /locus_tag="LZB08_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBM7314696.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            7150..7677
                     /locus_tag="LZB08_001186"
     CDS             7150..7677
                     /locus_tag="LZB08_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBM7314697.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(7748..8998)
                     /gene="clpX"
                     /locus_tag="LZB08_001187"
     CDS             complement(7748..8998)
                     /gene="clpX"
                     /locus_tag="LZB08_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBM7314698.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQSADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(9017..9601)
                     /gene="clpP"
                     /locus_tag="LZB08_001188"
     CDS             complement(9017..9601)
                     /gene="clpP"
                     /locus_tag="LZB08_001188"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBM7314699.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(9751..11037)
                     /locus_tag="LZB08_001189"
     CDS             complement(9751..11037)
                     /locus_tag="LZB08_001189"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBM7314700.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(11231..11701)
                     /locus_tag="LZB08_001190"
     CDS             complement(11231..11701)
                     /locus_tag="LZB08_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7314701.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(11701..13050)
                     /gene="rph"
                     /locus_tag="LZB08_001191"
     CDS             complement(11701..13050)
                     /gene="rph"
                     /locus_tag="LZB08_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBM7314702.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(13210..15234)
                     /gene="ligA"
                     /locus_tag="LZB08_001192"
     CDS             complement(13210..15234)
                     /gene="ligA"
                     /locus_tag="LZB08_001192"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBM7314703.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     INFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKEMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(15420..16358)
                     /locus_tag="LZB08_001193"
     CDS             complement(15420..16358)
                     /locus_tag="LZB08_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBM7314704.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(16460..16882)
                     /locus_tag="LZB08_001194"
     CDS             complement(16460..16882)
                     /locus_tag="LZB08_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBM7314705.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            17070..18353
                     /locus_tag="LZB08_001195"
     CDS             17070..18353
                     /locus_tag="LZB08_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBM7314706.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(18431..18946)
                     /locus_tag="LZB08_001196"
     CDS             complement(18431..18946)
                     /locus_tag="LZB08_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7314707.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(18946..20322)
                     /locus_tag="LZB08_001197"
     CDS             complement(18946..20322)
                     /locus_tag="LZB08_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBM7314708.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(20347..20898)
                     /locus_tag="LZB08_001198"
     CDS             complement(20347..20898)
                     /locus_tag="LZB08_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7314709.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(20956..21129)
                     /locus_tag="LZB08_001199"
     CDS             complement(20956..21129)
                     /locus_tag="LZB08_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314710.1"
                     /translation="MCTKKEKQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(21140..21973)
                     /gene="fdhD"
                     /locus_tag="LZB08_001200"
     CDS             complement(21140..21973)
                     /gene="fdhD"
                     /locus_tag="LZB08_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBM7314711.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(22022..24166)
                     /locus_tag="LZB08_001201"
     CDS             complement(22022..24166)
                     /locus_tag="LZB08_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23750..23752),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBM7314712.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(24417..25475)
                     /locus_tag="LZB08_001202"
     CDS             complement(24417..25475)
                     /locus_tag="LZB08_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBM7314713.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKRFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKSKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(25686..26345)
                     /gene="cprR"
                     /locus_tag="LZB08_001203"
     CDS             complement(25686..26345)
                     /gene="cprR"
                     /locus_tag="LZB08_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBM7314714.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         7956 a         4867 c         2939 g        10755 t
ORIGIN      
        1 cttaaaatta tataaacttt caataaacat tgttgtgata ttataaaaaa acataaatta
       61 ggaaaggaaa taataattat gaaaaacaaa taatggcttt ggagattatt aatatcattt
      121 tttagcaaaa aaaagtaaaa tggcatagcc tcaaagctac accaatttac taaaaattat
      181 attattatta acgttaattt taaacatata cgtttataag ttttcctcat attttaaaat
      241 tcttttaatt aacttatctc ttaatttccc tacttcatca aatctcatat taggactata
      301 gtaactttct ataatatcat gtttagactt ggctctctta agatttagta ctgcccaaca
      361 aattaaatca tctaaaactt tttcatcaaa tttaatatct tcttcatatt taagtagttt
      421 ttctctattt aaaaactgat ttaaatcaat tgtatcttct tctttaaaag tcgtacttat
      481 agttatgcct atatctaaat cagttaatag tacagtttca attttctctg gaataagtgg
      541 ataatgataa acttctacat ttaaaccttt ttcaatagct ttcttatata ctttgtttaa
      601 taatgtagtt ttcccagttc ctatttctcc atctaaataa tagactttct ttacatcttt
      661 taaaatgttt tctgtataat ctaaacaccc tacaggagtt atagctgaac caaataaatg
      721 cttttctctt tttagatttc ctgtgctttt aactccatca aatagccttt ttataaattc
      781 ttctgtgacc aaatttactt tgccataatc catacaatca gaatattttt cttctatgtc
      841 tctaaagatt ggttctgctg attttaaaaa cttaaatgct cttttaaatg aattactaat
      901 atctttatca taaactgata tttcttgttt attcttttct aattcttctg tattccagta
      961 atcacctaaa ttaattatct catccaaagc tccaggtagc tttgggtcca tagtatgtgg
     1021 ggcagttgca tccataagta ccacgcctaa tttctttata actaaaccat ccaacgaact
     1081 tgggtcagaa ggacaatgat ataaatcaat atcatatcct ctatgcaaaa actctttata
     1141 aatttttttc ataagtgagg attttcctac tccaggtcca ccctttaaac aaaaaactct
     1201 ttttacattt tcaggaatta tatagtcaaa aaaagaatat gagccttgtg atgtatttcc
     1261 acccgggaat gccttcttta cctttccttc catattagcg cctcctcata atttacaata
     1321 tctcttaatt aagatattca tattataaga attttattac agctttctga ttttgacaaa
     1381 tttaaaactt gccatctaat actaataaca ggttaatttt atctaattat tatagattct
     1441 catactgtgc cttattctat ctataaatac aatttaagtg tccatattga aatatttgta
     1501 ttgtaataca gctggatatt acttaaatcc aattgtttcc attataattt tatgttaaaa
     1561 taatattaca aaatacatct gtttttcttc ataaacgggt gaaattccct atcggcggta
     1621 aaagcccgcg agccttatgg cataatttgg tcatattcca aagccaacag taaaatctgg
     1681 atggtagaag aaaatagtat atgagtacct ttatgtaatt ttacatgagt aatctataca
     1741 aatccttcaa ctaccgtatt tattcatgaa attagacaca ttcaaggtac ctaatataca
     1801 ggtgcttttt ttgttgttta ttttacaatt atatcgtact tataaaatct attaagattg
     1861 gagtgttatc atgaaacaaa aatggatagt attgattatc atctgtattg gtgtatttat
     1921 gtctactctt gatggaagta tactaaatat cgcaaatcct acaattgctg ctgactttaa
     1981 aattaacatg tcacaaattc agtgggttgt aacagcttat atgcttgttg taacagctac
     2041 tatgttattt ttcggaaaac ttggcgataa agtaggttca aatcgtctat acacattagg
     2101 attttttata tttacaatag gttcttttct gtgtagtatg tcaaacaatt tatcaacatt
     2161 aatttcttca agaatctttc aggcagttgg agcaagtatt ttaatggcta ctgggcttgg
     2221 tatagtttcc aatgcatttc cagcaaatga aaaaggaaaa gctataggaa tcactggagc
     2281 tgtagttggt ataggaaata tgagtggtcc tgtaattgga ggtataattt tagaacattt
     2341 tggatggcct tctattttta ttatcaatat acctattggt attatagcag tctttcttgg
     2401 cattaaattt ctgcctaaac ctgttttgga tgaacaaaac aagagctttg atattccagg
     2461 tcttctactt ttcgcatctt gcactacgct tatattactt gcaatgaatg agaagggtaa
     2521 tacaagattg tatttaggta taacagcact tataatcttc ttattgttgg cattaagaga
     2581 agtaaaattt gaacaaagct ttattgacct acctctattt aaaaatagaa actttactgt
     2641 aggcaatatc attggagtag cttgttattt tcctcaaatg gcagtatctt ttctacttcc
     2701 attttattta gaacaattaa aaaatttatc tcctatgatg gctggttatg taatgactgt
     2761 acatccactt attatggtac ttatagcacc tattgcaggt tcactttctg ataaacacgg
     2821 tgcaaaaaac attctaacag catctttttc atttatgacc atctcactag ttggaatggc
     2881 acttttaaaa gcagattctc cattgtatct tttaattgta tgtttagtta tttttggact
     2941 tgggctagga gcatttagtt caccaaacaa tagtagtatt ttagcagatg ttcctcctca
     3001 aaaacaggga tacggaggca gttttcttgc aactattcgt aatttatcat ttgctcttgg
     3061 aacagcattt tttagtagtt tttttgcaca aagtcttaca tacaatcaaa agtttaaaag
     3121 tcacacatca gcctatgtta tagctagcaa tcagtcttat tggattgcag catcagtatg
     3181 ttttataggg cttatactaa ctgttttctt tatgagaaaa acagataaat ctatttctta
     3241 aatgctattt attgctgact acataagtca ataatttttt agttgaacta atttatacta
     3301 attttcccat accttactaa aatttattag tatttagcaa aattactgat atttattgtt
     3361 tataaacaat aaggatgcca aaaaaagttc attaaaacta ttttaaggca tcctatattt
     3421 tgctttaata ttaatataat taaaactaac tattattaca tttattattc aaacaaatct
     3481 tatgtcaata tttaacactt taaatttaat ctactgttat ttcatatcca tgttgattgt
     3541 attgatttac aatctcttcc cataattctt caacaccagt cttttttagt gaagaaacag
     3601 gaattacttt ttcactactt tccatttgca atttttttct tattatactt atgtgttttt
     3661 gatattgtcc tctagatatt ttatctgcct tagttgcaat aactacacag ttatatccaa
     3721 agtgctttat ccattcatac ataagcttat catctgctgt aggctcatgt ctaatatcta
     3781 caagtaggaa tatagaacat aactcttgtc tactttctag atatctttcc attatacctc
     3841 cccacttttc tttttcagac ttagctatct ttgcatatcc atagcctgga aggtctacaa
     3901 aataaaattc attatttatt agataaaaat ttatagttct agtttttcct ggtgtttgac
     3961 tggttctagc aaagtttctt ctgttaagca atgtatttat tattgatgat tttccaacat
     4021 ttgacctacc agccaatgct atctctggga ttccttctgc tggatactgt gatttattca
     4081 cagcactcat agttatctct gaacttctaa ttttcattat tttcaccact ctttaataaa
     4141 gcttgttcta atacttcatc catatgttct acaagcacaa actccatttt ttcttttaca
     4201 ttttctggta tttcatctaa atcagcttca cattctttag gaattaatac ttttgttata
     4261 ccagctctat gagctgctaa taacttttct ttaactcctc ctactgctaa gactctacct
     4321 cttaaagtta tctctcctgt cattgctata tttccaggca caggtctttt agtaagtgct
     4381 gagattactg caagagccat agttatacca gctgatggtc catcttttgg aacagcacct
     4441 tctggaatgt gtatatgaat atcattcgtc ttataaaatt cagggtctat atcaaacttg
     4501 tccactattg accttatata agagatacct gtcttcgcag attccttcat aacatctcct
     4561 aattttccag ttaatacaat ttctccttta cctttaagta cgtttacttc tacttcaagt
     4621 gtaactccac caacttctgt ccatgcaagg ccatttacta atcctatttg agggttaact
     4681 tctgcaagtt gatacttaaa catgtctttt ccaagatatg tctctaggtc agacttattt
     4741 ataacaacct cttcaagagt tgggtcttca acgtattttt tggcaacctt tctacaaatc
     4801 ttaccaatag ttctttctag tgttcttact ccagcttctc ttgtataatg gttgattata
     4861 cttcttaaag tttcatcatc tatttttata aagctttctt ttaaagcatg ttctttaatc
     4921 tgttttggaa gtaggtattt ttttgctata tttaattttt cttcttctat atacccagat
     4981 acttctatta cttccattct atctaataat ggtcttggta tattacctag actatttgct
     5041 gttgtaacaa atagtatttt tgataaatca aaaggtattt ctaagtaatg gtccacaaaa
     5101 tccttatttt gctctgggtc taatacttct agcattgctg aagctgggtc acctttataa
     5161 tctgctgcca tcttatctat ctcatcaaat aagaatacag gattttttgt ttgggcttct
     5221 tttaccccat taattattct tcctggaatt gaacctacat aagttcttct atgacctctt
     5281 atttctgctt catctctaac tccacccaaa gatattctta caaattttct atttaatgct
     5341 ctggcgattg attttactat agatgtcttt cctgttcctg gaggaccaac tagacatatt
     5401 ataggacctt taagtgactt agcaagagtt cttatagcta gatactctag tattctctcc
     5461 ttaacttttt ctaatccata atggtcttca tctaatatat cttttgcctt agttatatct
     5521 aatttatctt ttgtctcttt attccatggc aatgagaata tagtatctaa atagtttcta
     5581 cttacagata catcaggaga catagaagat atttttgaaa atttatctat ctctttttct
     5641 attttctctt tagttgtctt aggtgctttt atttttttaa gcttttctct atactcatct
     5701 gcttcagagt ttatatcttc ttcttcacct aattctttct gtatagcttt taattgttct
     5761 ctaagataat attctttttg aaccttattc atctgttttt ttactcttaa agttattttc
     5821 ttttctattt ttaatatatc tatttcctct aataatatag aataaataag ttcaagtctt
     5881 tttctaatat caaattcttc taatatttct tgtttttggc ttgattttag atatatgttt
     5941 gcagctatcg tatctataaa tctatctaca tcttctatat cagctaaaga tattaatatt
     6001 tctggtgata ccctattgcc aatatttata tactcttcaa aggcatcaaa tacatttctt
     6061 acaaatgctt caatttcaac ttctgtttca ctatccaaat tatcactatc aaatactatt
     6121 tcttctatta ctgctctgaa atacccatct tcttgttcta ttttctttac tctacccctt
     6181 gatacacctt caaccaaaac acgaactgta tctcctggta attttatcat ttgcttaact
     6241 ttacagattg ttcccacatg ataaaaatcc tcttctcctg gctcatctac ctctgcttct
     6301 ttctgagaag ttaaaaatat aagttcttca tccatcatag cttggtctaa agctttaaga
     6361 gatatctctc tacctatatc aaaatttaat atcatatatg gaaatatagc taatcctctt
     6421 agaggaatta atggtaattc atgatctatt ttagtataat tttgttccat caacattact
     6481 ccttttctag tctaattgct ttgcttttaa tattatatat ttgaacaagt taattttcaa
     6541 cattaatgat aaattcttag aatactatca tactatatta tctttttatt atgatataat
     6601 attaatatta gtatacttag atgttattta tcttttggag gtttctatta tgttgaaatt
     6661 gtttttaacc tttttaaaaa ttggtgcgtt tacatttggt ggtggatacg ctatggttcc
     6721 tcttatacaa gatgaacttg taaacaaaca aaaactttta gagcaggaag aatttattga
     6781 ttatttatcc atagctcaaa gttatcctgg agtactggca gttaatatat ctgtattatt
     6841 aggatataaa ctttatggta taccaggagt tcttatatgt accttaggct ctgctttacc
     6901 atcattttgt atagtcttag tcttatcgta tctatacttt aaaaattcat catctaaaat
     6961 tttagatgga ttttttaaag gagttgcgcc tgttgtaata gctcttatat tgtattcttt
     7021 tacgagcatg ttcaagaaac taccaaagtc taaaactaac atactactat taattatagc
     7081 agttttatct gttggtgtat tcaatataag ccctatttat ttaataattg gaggaggtgt
     7141 atatagtcta tgtcagaaat aatactactc ttcttaatat tccttaaaat aggggcattt
     7201 agttttggtg gcggttatgc catgctacca tttatccagc aagaatttat atctaaatat
     7261 cactttataa ctaatcaaga gtttttggat ttacttgcta tatcacaatc tacaccaggt
     7321 cctgtagcaa taaatagtgc tacatttgta ggatttaaga ctgctggctt ctttggttct
     7381 ctagctgcaa ctattggtgt tacaattttt gcattaatag gtctaactat tatatcaaag
     7441 ttattttcta aatttaaaaa taataaaaag gtagaaaact tattgacagt tcttagacca
     7501 attactctag gattcatatt atcagcagct ttttcatctg ccaaagaagt taattgggac
     7561 ttatatggaa taattgcatt tatagcatca ttttatcttt tatataaagg aaaaattggt
     7621 tccattgcaa ttgtattcgt ctttggtatt ctaggtatat tactcacatt tttataataa
     7681 tgatacttat tttatattta gcatattaac ataaaaaaag gagcttttaa agctcctttt
     7741 catcatctta tgcactttcc tcttgatcct taagtactat gataggattt actgagtctt
     7801 cgttcacaga tttctctgtt actataacct tctttatatt atctctagat ggtacttcaa
     7861 acatagtttc catcattacg ctttcaacta tacttctaag tcctctagct cctgtatttc
     7921 tttctatggc ctttttagcg attgcacgaa gagcaccttc ttcaaattct agctcaacat
     7981 catctaactc caataacttt ttatactgtt ttactagagc atttttaggt tcctgtaata
     8041 tctgcattaa tgcatcttca tccaacaact ctaaagtagc taaaactggt attcttccta
     8101 taaactctgg tattatacca tattttaata aatcctctgg aagtactttt tcatatagct
     8161 tccctaaatc taattctttt ttactttcta ttttagctcc aaaacctagt gttttatctc
     8221 caccacgttt ttgaattatc ttctctaatc catcaaaagc tccacctaat ataaataata
     8281 cattagtagt atctatcttt aaaaattctt gatgaggatg ttttcttcct ccttgtggtg
     8341 gaacatttgc aacagtacct tctaatattt ttagaagtgc ttgttgaaca ccttcaccac
     8401 taacatctct agttatagat gggttttcag attttcttgc aatcttatct atctcatcta
     8461 tatatattat acctctttca gctttttcta tatcaaaatc agcagattga ataagtttta
     8521 aaagtatatt ttcaacgtct tctccaacat atccagcttc agtcaatgat gtagcatctg
     8581 ccattgcaaa aggtacattt agagttcttg ctagagtttg agcaagtaat gtctttccag
     8641 aacctgtagg tcctaataaa agtatattac tcttttgaat ttctatatcc ttactactag
     8701 attttttact gtatattctc ttataatgat tataaaccgc aactgataaa gcttttttag
     8761 ctttttcttg acctattaca taatcattta gaatctccat catttccttt ggttttggta
     8821 aagatgtagt atcttcatct atagtatctt ctatttcttc ctgaataatt tcgtcacaaa
     8881 gttcaacaca ctcgtcacaa atatatacat ttggacctgc aattaatctt cttacttgat
     8941 cttgattttt accgcagaat gaacacttta attgtcgttt ttcttcatat ttagacatat
     9001 taacacctct ctctatctac ggtctctttg taaatacttc atctattaaa ccatattctt
     9061 tggcttctag tgcactcatg aagttatcac gttcagtatc cattttaatt ttttctaatg
     9121 gctgaccagt tctttcagat aatatctcat ttaaagtttc ttttattttt aaaattctct
     9181 ttgcatgaat ttctatatct gttgcttgac cttgagctcc acctaatggt tgatgtatca
     9241 ttatttcact atttggtagt gctagtcttt ttccttttgc tcctgctgcc aacaagaatg
     9301 ctcccataga agcagccata cctatacata tagtacttac atctggtttg atgtattgca
     9361 tagtatcata tatagccatt ccagaagtta tacttcctcc tggagaattt atatataaat
     9421 gtatatcttt gtctgggtct tcagcttcta aaaataatag ctgtgctact ataagccctg
     9481 ctgtggcatc gttaacttga tctcctaaga agattattct gtcttttaat agtctagaaa
     9541 aaatatcata agatctttct cctcttcctg tttgttcaac aactacaggt actaatgcca
     9601 tggtttcata ccccctttat tctatataat ttacagtagc tttttttaag ctactgtaaa
     9661 ttattatacg catttaacta aattatattt gcacatcatt atatactaaa catttcataa
     9721 cgtcaactat attagttagc gtttatttta ttaagctatt gttgcattat caactaataa
     9781 atctattgtt tttcttattt ttatttgacc ttttatatct tctttgtcag catctcttaa
     9841 agaagcttct attttttcta cttccatatt gtaagcagaa gccatttttt ctaactcagc
     9901 cttaaattct tcttctgttg cttcaattcc ttcagcttta gttattgctt ctaatactaa
     9961 agaagactta actaattttt tagcatcttc ttttctctct tctcttaatt cttccatagt
    10021 tctaccagtc atctctagta attgttgtaa tccaaatcct tgatattgta attggtagtt
    10081 aagttcattt aacatattat ctatttgatg ttgtatcata acttctggta tttctatttc
    10141 tgtattttca gcaacttttt ctactactga atttctagtt tctgcatctg ctctattttt
    10201 agcttcttct tctaacttag ctcttacatc tgcttttaat tcatctaaag aatcaaattc
    10261 actagtatct ttagcaaatt catcatcaag tgcagataat tcttttactt ttacatcatt
    10321 tatttttaca ttaaatacaa ctggttttcc agctaaatct tgtgagtgat attcttctgg
    10381 gaaagtaaca ttaacttcta cttcttcacc agctttttta cctactaatt gttcttcaaa
    10441 tcctggtata aatgtgtttg aacctataac taagttataa ttttcacctt taccaccatc
    10501 aaaagctaca ccattttcaa aaccttcgaa gtctattata gctgtatctc catcttctaa
    10561 agctttgtct tctacactta ctaatctggc atttttttca accatttctt gtagtttagc
    10621 atcaacattt tcgtcactta cagtattctc aaccttagct atttctatac ccttgtagtt
    10681 tcctaattca aactctggtt taacttctac attaagaacc ataaccaacc cattgtcttt
    10741 acttatttct tctatatcta aatctggatt gtctattggg tctatgtcta attccttgat
    10801 agcttctgga taaacttctg ggaataatat ttctatagca tcattgtaga atataccttt
    10861 tccgtattga gtttctataa caattcttgg agctttacct tttctaaatc caggtatatt
    10921 gtatttattt ctgcttttat tataagcctt atttactgct gcttcaaatt tattgttatc
    10981 tactgttatt tttaaagtaa ctttattacc ctctttttta attagttctg ctttcatcct
    11041 tctaatcctc ctagtgatat ttatgtaata cccgataacg gtattataat tcattctatt
    11101 tataattcaa ccacttaatt atatcacata agttttttta tttacaagtg ttccattaat
    11161 atcgttgtac ttatttgata aaatttaatt ttttgagcgt tttttgccat ttttttagat
    11221 aaatacacct ttatatgcgt atttcttcaa tttttatgtc atcttcttct atatccataa
    11281 tgacaaaact tttatacgat actcctcttg gaagtgaagt gcttccagga tttatgtaaa
    11341 gtacaccatc tttaatttct ctaaatgggg tatgggtatg gccaaatata actatatcag
    11401 catcaacttc agttgccttt ttttctaaca tattagttcc atattttaca ccatacttat
    11461 ctccatggca taagaatatg gttttatttg ccacttcaaa gacaacttct tcctcaacag
    11521 catcaaagtc acaattacct ttaactgcaa taatacctac attagtcatg ctatgaatat
    11581 atcttgaatc agtaaagtta tctcctgcat gaattattag gtcacattct ttcaaatatg
    11641 gtattgtttt ttccatattt ttaatcatca tatgtgtgtc gcttactatg cctattttca
    11701 ttacttcact acccttttta taaactcttg tttcataagt tttagtgcgt ttgctctatg
    11761 actgattgaa ttttttataa cactaggtat ttcaccaaaa gtcttatcat atccttttac
    11821 tataaataag ctatcatatc caaaaccatt tttacctttt tcttcaaatc ctatcattcc
    11881 ttcacaaatt cctcttacta caaattcttt tccatcagga aaaactactg ctattgcaga
    11941 gacgaatctt gcatttcttt tgctcattgg tacattttgc atagctttta acagtttttt
    12001 tctattttct tcatctgttg catgttctcc tgcatatcta gctgaataaa caccaggttt
    12061 tttgcctaaa gcatccactt ctaagccaga atcatctgat attgctataa gtttagtctt
    12121 ttttgctatt gttctagctt ttatcaacgc attatgctca aatgtttttc catcttctac
    12181 tatctctata cctgctaaat caacatcttt taaagagtac actttatatt caaaatcttt
    12241 taagatttca cctatttctt ctagcttatg agcattatta gtagcaatca ctacttcatt
    12301 accataatcc atacccaata tctcatctga tatttcacct aaagcttctc tttgaagcct
    12361 tattaattct ttattccctt tttcacctaa ctctaacaat aaatttaaat ctgctctcga
    12421 aaaaggtttc tcttctcctg tcccctgtac ttctacaaac tcacacttat cagtcattat
    12481 tatattcata tctacctgtg ctttagaatc ttcttcataa cataaatcta gcatgtgtat
    12541 tccatttaca ataccaacac taattgcaga tacaaaattt gttattggca tctcttttat
    12601 tgtctttgct ttatataatt tatatattgc ttctgcaact gctacaaatg cccctgttat
    12661 agatgcagtt ctagttccac catctgcctg aatcacatca caatctaccc aaatagttct
    12721 ttctcctaat ttattcaagt ctacaacaga ccttattgct cttcctatta acctctgtat
    12781 ttcttgtgtt cttccatcaa tttttcccct agatgcatct cttatctttc tttgttgtgt
    12841 tgctcttggt agcatagaat attcagcatt tatccatcca gtaccactat ttcttaaaaa
    12901 aggaggaact ttttcttcca ctgatgcagt gcatataacc tttgtctctc ccatttctat
    12961 aagaacagaa ccttctgcat actttgtata atctctagtt atctttacag gtcttatctg
    13021 ttcattactt ctatcataac ttctggtcat tttttatatc ctttctcata ttttttattt
    13081 tttataaatt tatttttata atttttaatt ttgtcaaata ttaattattg tcatttttcg
    13141 taatcttatt agcttactga actattctat cattaattat agtagttttc tactaaatta
    13201 agcgactttt taactctcta atacagcttt tacttcatct ttcgatgata actttaataa
    13261 ttctaaaaat ctctcttcat ctataacttt aattcctaaa tcatttgctt tagtaagttt
    13321 agaaccagct tcttcacctg ccaaaacaaa tgttgtcgat ttactaacac tagatgtagc
    13381 ttttccacct cttttttcta tcatttcttt tgcatcattt ctttttaatg taggaagtgt
    13441 accagtcaat acgattttca tcccttcaaa tattttttct actttttcac ctgtattatc
    13501 caatgatttt gtatttactc ctacattttt taacttgttt ataacgttca tatttttgtc
    13561 ttccttaaaa aactcgacaa cgctgtttgc cattatactt ccaaattcct ctaattcttc
    13621 tagttgctca ctagttgcag aaattacttc atctaaatcc ttaaagttat atgcaagtat
    13681 cttagctgct ttaactccta taaatttaat acctaatcca tttataagtc tccataaatc
    13741 attttctttt gatttttcaa ttgaatttat taaattgtca gcagattttt cgcccattct
    13801 ctctaggtcg actacatctt ccttttttat ataatataaa tcagatacat cttgaataat
    13861 tttttcattt aatagcagtg taattataga ttctcccaat ccatctatat tcattgcatc
    13921 ccttgacaca aagtgtataa ttccccttct tatttgggct gggcaagaca tattgataca
    13981 ttttacagca gcctctcctt ctaatcttat tgttggctct gaacaaactg gacacttttc
    14041 tggcatttta aaatttattt catctccagt acgttcttct tttataactt ctaaaacttg
    14101 aggtattatg tcccctgctt tttgaactaa tactgtatca tttattttta tatctttttc
    14161 tcttatataa tcttcattgt gaagtgtagc tctacttaca gtagtacctg caagtctgac
    14221 tggttcaaga attgctgaag gtgttattgt accagttctc ccaacttcta ctattatatc
    14281 tactatttta gttttcttct tttctgctgg gaatttatat gcaattgccc atctaggact
    14341 tttggctgta tatcccattt cgtctctttg ttttatattg tttactttta ttaccatacc
    14401 atctatttcg tagtctaaat tacccctgtt atctgtccag tattcaatat gctcaataac
    14461 ttcatctata cttttacaaa ctttataact ctgacttact gaaaatccta gtttttctaa
    14521 gtactctagt gattcactgt gactttctaa atcgtattct tctatatttt ccatattaaa
    14581 tacaaatata tctaatggtc tctttgctgt tagttttggg tctaattgtc tcaatgaacc
    14641 agctgcaaga ttccttggat tagcaaataa tggctgttct tgttcttctt gttgttcatt
    14701 tacttttcta aaattatctt ttgatatata tacttctcct ctaactatta gttctttttt
    14761 atcatttatc ttaagaggta tgctttttac tgtcattaag ttttgagaaa tatcttctcc
    14821 tacaactcca tctcctctag ttgctccttt ttcaaactct ccattgttat aagttagacc
    14881 tacagaaaga ccatctattt taaattcaac aacatattca acattttctc ccacatattc
    14941 ttgaactctc ttatcaaaat ctctcaaatc ttgctctgaa aaagcatttg ataaactcaa
    15001 cataggagtt ttatgtacaa tttgattgaa cttatctaat ggcttacctc ctaccctatt
    15061 tgttggagaa tcaattctct ttagttctgg atgttcttct tctaaagaaa ttaactcctt
    15121 cattaactta tcgtattcat aatctgatat ttctgggcta tcttgattat aatacctttc
    15181 attatggtag tttattgtct ctataagttt attaatttca tcttttacgc tcactttttg
    15241 cacctcttca tatttttaaa attaaattat tttgaaattt ccctataatt aattatctcc
    15301 tatttatata ttttttagta tatcactttt ttggctttaa atttattaca aaaaaaactc
    15361 ttattagtat ctttaatacg actaataaga gtttttattt ttatcaaaat cttttatttt
    15421 taatagcctc ttgatatgtc tacaacatta tctacaggtt tattctccat tatatagtgt
    15481 ttcaaattat tataaaccat attaaatgtt ctctctttgt ttttatctga cacccaagaa
    15541 ttatgaggag ttactatgac attttcgcac tcccacaact tattgtcctt gtttaaaggt
    15601 tcactttcaa atacgtctaa tgctacccct ctaaatttct ctacacattc ttctaaatct
    15661 ttctgattaa ctgtatttcc tctacctaca tttataaata cagaaccatc tttcataagt
    15721 ttaaacatat ctttgtttac tattccatct gtactttttg tagaaggcat tgctacaact
    15781 actatatcac attcttcaaa tacaatattc atatcctcag tagcaaaaca cttatcaaag
    15841 tattttttac tacttccatc agtattgaca ccccatattt ctacaccaaa agcttttaat
    15901 ctttttgctg cttctgaaga tatagtacct gttcctaaaa atccaactct ttgtccatac
    15961 aattctgata cagaaaaatc catcttccat tctttatttt gttgttgttt aaaaaatttt
    16021 gcactatttt tatatatttc taaaatatac aatacaatac tttcaccaat aggtacacta
    16081 tatccgcctc tattattagc tatcttaata tttctcttca atacctcttc ttttggaagt
    16141 tggtctatac ctacactagt tgtctgtata tattgtaaat ttttcatctt agaaatatct
    16201 aatctctcaa atggattgta tgtaactagt acatctgctg tatttacatc ctcattattt
    16261 tcaatactat cttcagagta atgtatgaca tcatagccta attctcttat tttatcaaat
    16321 ttttcttttc cataatttat tgtaaataaa actttcatct caaatctcct ccatctaccc
    16381 acatttaaaa ttttatattc taaaatataa tatgatactc aatgaacatt agttaaaatt
    16441 actataagat atcttaaatt tacttatgtt caattgtttt tcctaaagac atagcattac
    16501 gttcttgaac taaattttct attaaattat tcaatgtcca atcaatagat gtctctgtta
    16561 ccatagctgt aaaaggtcta tttattctca ctttttttag cccttctaaa aatgcattta
    16621 cttgtgtgct tggagtgtta ttaaatatta cagacttact cttaatccca ttttcacatt
    16681 cacttgatat atttccatct aaaatgtctc ttattatatt ctctccattt ttatcactta
    16741 agatgatatg gtctcttatt ccagcaaagt tacttacatt tttaattaat gataattctt
    16801 ttttattaaa gtttacaagc ataatacact tcctgttgtg tattttatct aggttattat
    16861 tatttatttt ttcaaatgac attttatcca tcctttcatt tatttatata ctttaattta
    16921 ccccatacat ttaaaataat caatttttat agttaatttt tatattaata cccaaattat
    16981 atttaaatag ttaggctatc caaaactaat atataaaatt agaataatta gattaatatc
    17041 tacaaatcat aacctaaagg agagatacca tgaatacatt actaaacaat aaatttagca
    17101 ttaaaaactt ttcaaatgct tgggttatag tacttggttg tatgttgata caagctgttc
    17161 catttagtat tgtagcaagt atacagcctc aatttataag ttatgttgta gaaggagaag
    17221 gctttagttt agcttctttt tctctaatat tcactattgg tactattgcc tcagcaattg
    17281 catcaccttt tataggtgta ttattttcta aagtcaatat taagatacta tatttattag
    17341 gttgtataat ttctagtatt gcttttgcta ctttttctat atgtactgaa ctatggcaat
    17401 tttacactgt atcatgtatt gttcaaattg gcgctacaat tatatctgct ataggaatac
    17461 ctgttttaat aagtgcttgg tttgatgaag aaagcaaagg aaaagctttg ggctttgctt
    17521 ttgctggtgg ttctattggt agtatatttc tacaaccact tgcagttcgc ttattagcaa
    17581 ctcaaggata cagtcgttct tatctaatat ttggaattat agcattaata gttggtatac
    17641 ctgtaatatt attcatactc aggatgccta aagatattaa tgaaaaggta aaatctaaag
    17701 gtaaaaaaga gaaagcatct gatacaattt ctccttcagt atggggttat acattaaaag
    17761 aagctaaaaa aattaaatat ttttggatta tgactattgg atttatattt gttggtatat
    17821 atgtttcatc attatccgta caatatccaa cttatcttag aaattatcta aatcctattc
    17881 taataggaac agtaggctcc acatttgctt tatttgctct cattggtaat ttatttggag
    17941 gattattgtt tgataaattc ggtcaaacaa agccacttat tatatctttt gtattggttc
    18001 ttatagcaac tttatcatta ctattggtag ataaatcttc tacttttgca tttgtatttg
    18061 ccattttgaa aggtctttct gtatttgctt atatgattgg tcctgcttat ttaactggct
    18121 ctttctttgg tcaaaaggag tttggtagca tatatggaat cgtacaatta atttttgcag
    18181 tgggatttgc attaggttca acattatttg gattgttagt tgataatttt ggatacagat
    18241 tttcttggtc tattataatt ttatcagtta taattgccta tactttactt attataactt
    18301 ctataggaat gaaaaaattg aataaagaaa gattagataa actaatgcaa taattgattt
    18361 ttagatatat aaatattata ataaaaaaga tggagctttt aatcataaat aaaactccat
    18421 cttttttata ttacaatata gctaattttc tcttattttt aactttacta tctatatcct
    18481 catccttaac tatagtaaat gccttagttg gacaaacatt tacacatgca ggcccttctg
    18541 gcgattctat acaaaaatca cacttatttg ctgtcattct ttctctaaag caaagctttc
    18601 cttcatctac atcttttaat gtaactatgt ctattgcacc tataggacat gctaacatac
    18661 aagtcttaca tcctatacaa ttttcttcat tgatttttat tgtgttacca accctaacta
    18721 taccaccatt tggacatgcc tttgcacaag gagcatcttc acaatgtcta cactgtattg
    18781 gagcacttac ttcttttgtc tttatgacag aaagttttgg attaaaattt atttcatctg
    18841 gattttggta aaaaatatct tcatctgaat gtgcaactac acatcctatc atacaagttc
    18901 tacatcctat gcatttatca gggtctgcta taacaaaata gttcatcact tagccacttc
    18961 ctttcctata caatatgtag tgtgtaatag atgatgagat ttttctccta atggttctac
    19021 cagatattca tcatacagtt ttttaatatc aggattctca tgagattttc taattgctaa
    19081 atgttcatca tgcacgtatg ttgcttttgt tctattttca taagccaatt ccttatattc
    19141 ttctagtaaa atttttggtt gacctccacc acttacacac ccaacaggac aagtcataac
    19201 ttcaataaaa tctacatcta attttccttt ttccaagtct tcaagcactg gtattacatt
    19261 ttttaatcca cttactactc caactcttaa gtctaagtct cctattttaa tagttgactt
    19321 tctaaaacca ttttctcctc ttacttgttt tacttctaca tcaggaatac tttctccagt
    19381 tataagctca tatcctgttc ttatagcagc ttccataacc ccaccagtaa caccaaagat
    19441 agttccagct ccactataac tacctaaagg actatcaaat ttttcttctc ttaaattttt
    19501 aaaatcaatt cccatgtctt ttattaaata agctagttct ctagtagtta atactacatc
    19561 tacatctcta tatccactag aatccatttc ttctctatca cattcatatt ttttacatgt
    19621 acatggcatt atagaaacac taaatatctt tgatgcatct acattattta tctctgcacc
    19681 ataagtttta aatattgcac cacccatctg ctgaggtgat ttacaagaag ataaatgatt
    19741 agttaattta ggataatttt tttccatgaa ttttacccaa gctggacaac aagatgtaaa
    19801 cattggaaga gttccacctt ctgtcactct tttaattaat tcactacctt cttccattat
    19861 agttaaatct gctgcaaaat tagtatcgta gactctatca aaatttaaag ctctaagtgc
    19921 tgctgccatt ttcccagcac ctaagtatcc taaataatat ccgaaatctt ctcctaaagc
    19981 aactctaact gctggtgcac attggaccat agtgaataca tttgggtcac ttaaagcttt
    20041 cttcacctta tctatatcac atacattgta tgctgcaaat ataggttcat ttatagactc
    20101 tggtatctct ctttcttgtt ttttctcttg taaaaactca aatccttcat ctataactga
    20161 ggcataagat ttacaagttt gaacacattg accgcacata acacatctat ctaaatttat
    20221 ctcttgtggc tttccctctt caccttcaat agcatttact ggacagacct ttgaacattc
    20281 tctacatcca gtacataaat ccttatctat atttattatc ataaacgtca cctacccctg
    20341 tatcctttat ttatttgtta acaataaact aagtgcagct tgtctattct tagctttttt
    20401 gtcatctatt ggagtaacaa gttttaatgc ttcctgagga catgcactta tacaagctat
    20461 tttttcattg tctttacata agtcacattt ataagctatc ttcttatttt catctaaaat
    20521 tacttgttct acttctttac catcttcata ttgtggaagt aagtctatag ctccaaaagg
    20581 acatgctagc agacaagttt tacatcctat acacttttct tcatttacag acataatatt
    20641 gttttcttta actatagctt tttgtggaca tgagtttaaa caaggagcat cttcacaatg
    20701 tctacactgt attggcatac agaaattgtc accttttact aagtaaagtc taggtataac
    20761 aggtatatca actgttccaa ctgtataacc cacattatta tgctgattat gcattgtaaa
    20821 acaagctact tcacaagctc tacaacctgt acatttgttt gggtctgcta ttacaaaaga
    20881 gcctatttta tttttcatat tatcccacct ttcaatttgt atatttccaa ccctctagtt
    20941 attttcatca tatgtctact tactttcatc atatatattt atatatttca ctattttctc
    21001 agtacttata tttcgtttac attttttgca caaattgtat gtgttattat acaccttatc
    21061 agtatcatca attaatactc cacagatttc acactttgaa tatctttgct tttctttttt
    21121 agtacacata tattcattct cactttttga taactctctg tggatttgca taaatattca
    21181 tccgtcttcc tcttacaaat ccaataagcg ttatatttaa ttttttagaa agctctattg
    21241 ataaattagt cggtgctgat tttgatacta caataggaat ttgagtcata gctgctttta
    21301 atatcatttc caatgatatt cttccactta cagccacaat tttatctttt aatgttatgt
    21361 catttaaaac acaaaatcca atagccttat ccattgcatt atgccttgct acatcttcac
    21421 aagtgactat ttttttatca aaattaaata cagatacgct atgtacccca ccagtatttt
    21481 taaataattc tgatgaattt aaatttttat ccattatact gtaaatcgta tcataattta
    21541 tttttatatt gctctttaca ggagcacatg ttatatagtc tagtgaattt aaaaatataa
    21601 tttgctcatc ttgatttttt tgtttctctt gaatagttac ttttgcaaaa cgttcactct
    21661 catttatctc aaattttaat aaactttttt tgttttctat atatcctttt gttttcaaga
    21721 atcctactat aagttcctct aaattatatg gggtacataa aaatgtattt acatatttat
    21781 catttaaaat aaaactaagt ggatattctg caattatttc ttcattatct attgatactt
    21841 catgttcatt tattttgctt acttctacat taaaagaatt taaatatttg tagtccacta
    21901 gactataact attttcaaaa actttatcct tactattttc ttgtaattta gtttttgagt
    21961 atatattgtt cataaaatat tagcctttct gccttttata aatcttaaat ctaataaatc
    22021 attagatatt tgtagctgtc attttgtctt tcaaactttg atactcttct cttacacatt
    22081 tttctgctaa ttcttggtct tcaagttttt caagtttaac agcacaatat ttatactctg
    22141 gtgttttaga tatagggtct aaatttgcta ttgtaagttc attgcaagct ccaacccacc
    22201 actgataagt catatatgtt gcaccttcct taactctatc agtaactgtt gctcttgtta
    22261 ttacacttcc tcttcttgaa ctaattctaa ctaattcatc atcttcaatt cctaattttt
    22321 cagcatcatt agaatttatt tgaacacgtc ctggttcatc ttctaatgaa ctaagtgttc
    22381 tacaattacc agtcattgtt cttactgaat agtgtccaac ttctcttaca gtacataaac
    22441 taaatgggta ttcatcatct tcaacttcca tagggggtct ccattcagca gcgaacaaat
    22501 tacctttacc attaggagta gaaaactttt gaccttcata cagatacata gttcctttat
    22561 cttccatact ttcactctta caaggccatt gaacgcatcc ttgagtttct atcttttcat
    22621 aagtagcacc taagaaactt ggacataatt gtctaagttc gtcccatatt tccttagtat
    22681 ttttataatt cataggatat cccattgctg tagaaatttc gcttataatt tgccagtctg
    22741 gttttacatc tccttgtggc tctatagctt ttctcatcaa ttggaatcct ctatctgcac
    22801 atgtatatac tccctcatgt tctccccaag aagtagctgg aagtataaca tcagcgtgaa
    22861 gtgctgtctt attcataaat atatcttgaa caatgacaaa ttccaattca tctaatgctt
    22921 ctctaacttc tgatgcatct gggtcacttt gcactgggtc ttctccaaat atataataag
    22981 ctttaagctt tttctctttt agtactaaat ttggaacttg agttaaacta taaccattat
    23041 taggagataa ttttaccccc caagcttttt caaacttttc tcttatctta tcatcagtta
    23101 cattttgata gccaggatat acattaggaa gagctcccat atcacaagct ccttgaacat
    23161 tattttgtcc acgtacaggt cctattccaa cgctttctct accaaaatta cctgtaagta
    23221 gagcaattga tgcaagtccc ttaactacat caacagcttg accaaattga cagacaccca
    23281 ttccataaag tatcatagct tttttacctt ttgcatattc tctcatagct tttcttataa
    23341 gctcttctgg gattcctgta atcttttcag catatttagc tgtatatggt ttaacaattt
    23401 ctttatactc atcaaatccc tgtgtatgat tttgaacaaa ttctttattg taaagacctt
    23461 cttctattaa tacatttcca aaagcattta ctaaaaccat gtttgttcca ccttttatag
    23521 gtagatgtat atcagcaatt cttgcagatt cagttactct agggtcagta acaattaatt
    23581 tagcaccatt ttttttagct tttactattc tatttgccac tattggatgt gaatctgctc
    23641 cattatatcc aaatataaat aaaacatctg cattttctat ctctggtata gaattagaca
    23701 ttgcaccact acctaatgag taagccagac cggctacaga tggtgcatgt cagacacgag
    23761 cacaatggtc aacattatta gttcctattg tagctctcat aaatttttgc attatatagt
    23821 ttgcttcatt accaggtcct cttgcagaac ctgttcccat aatagaatca ggtccatatt
    23881 tttctttaat ttcatttagt cttgaagctg tatagtttat agcctcatcc cattcaacct
    23941 cttctagtac accttgtttt cttatcattg gcttttttag cctaggtgtt agaattttag
    24001 ggtcatttag aaaatcccat ccataacgac cttttaaaca caaactttct tgatttgttc
    24061 taccattagc cggttcagct cttaatatct tattatcttt tactactaga tataattggc
    24121 atccagctcc acagtatgga caaactgtta aaattttttt ctccatacaa ttcccccctt
    24181 agatttatcc cctgataagt aaaatttatc ttaaagataa tgagtgtttt tataatatgc
    24241 aaaatcataa atattttgca atactatcta ttaaattcaa caattttctc acaattcctc
    24301 ttaaatttca aaaaaaatat gtcataataa tctttatatt tcaaccattt aattaaaata
    24361 ttctagttct tatgacatat tttttaatat actcaatata tattctttaa acttatttag
    24421 ttttcactta aaacaatatt tctgccaatt ctatttttca ttctatagtg agtaattaca
    24481 gtaaatattc cacttccaat cagaatcaaa tagtacataa atccaccata taaaaatact
    24541 gcatatccca tcaaaggttg agggaatact gatttgaata tagtgaaaaa ggctaattca
    24601 ttagctccta cattcccagg tgttggtatt ggagatattg ccatatataa aaatgcttgt
    24661 aatgtcagta tataaatata gctataacct tgtaaattaa atgctttata tatccaaaat
    24721 gaaacactaa agtaagcact taattgaata aaagttacta ttatagttga aattaatacc
    24781 tttttactct ttgcaaaaaa atttattgct ttactataat catctataaa actttctatg
    24841 gattcaatct tattatctaa aaattttaaa aacttaattt ttgataaatg ctttatagta
    24901 attctcataa aatattttat ttttttagga tttaaaataa ctaatattat cataattaag
    24961 acaaatgaat taatggctat ccctagaaac actagaggca taatcatttt catttgtttt
    25021 tctaacatag caaaattcat taaaacaagt attgtacaat aaaaagtaac tactatttgg
    25081 aagataatag acttattggt aacaactgca cttgccttac taagtggcat tttacatttt
    25141 ttaagtacat atatctgtac tggttggctt cctgaagcaa aaggagttat taaattataa
    25201 taaaatccca taatggctag cttaaaacca ataaatctaa tattaacttt gtgagtgctt
    25261 tctataatta tcttcattac tactccttca agcattatgt gacacattat tgccaaagca
    25321 cctatgaata aaaacctctt atctaccata accataacat ttgatagcat tttaatatcc
    25381 aatgttttaa acactaggta aattgttata cctattaaca aaaataaaaa tgtatactgt
    25441 agtaaactct ttctaatatt ctttagtcta tccattagaa ataaccatcc tttgctcaag
    25501 atttatacta aatactatat tattagtata cactatgaat cttaatattt tataacaaaa
    25561 taccttacaa ttttgtcatt tacaaaaaat attaatctat aaattaaatt ttaatatata
    25621 gattaatatt ttacaaacat tttgtagata tatataatat tttcactaaa caacacttat
    25681 acttattaca cccatttata ccctatcccc catacagttt ctattgtata aatatcatat
    25741 tgttttaatt tgcttcgtat attttttata tgctctgtga tagtacttat atcactttct
    25801 ccatcaaaac catataccga ctcatatatt ctttcttttg aaaatacttg tcccttattt
    25861 tttgctagaa attcacatat tagatattca cttttagtaa aattaatttt tttaccatca
    25921 attgaaactt ctttgcctaa aaagttaaat tgaacatttg agatagttaa taaattcttt
    25981 ttttctctac tttctcgcct taaatgagca gttatcctag cccttaattc tcctacacca
    26041 aaaggtttgg ttatatagtc atctgcacct ataccaagac cgtacataat atcactttcc
    26101 atattttttg ctgtcaaaaa cagtattgga caatcaacct tatttcttat agttttacat
    26161 acttcaaaac catccatctt aggcatcata acatctaata ttattagatt aaaataatta
    26221 aaatccatgt tcatagcctt ttctacatca ctaattacct ttacttcatg tccatctctt
    26281 ttcaacacat tttcaatcaa ctttaacata tctaactcat catctactac taatatctta
    26341 gacataataa ctcctccatt tatccatcta atagcattct aatttatata tttaataata
    26401 tctttaaaat tcacattaac cataatactc tcatattata gcttacaata attaattact
    26461 attttacaaa taaaatattt tagatacaaa aactttatat ttcaaaaact ttatatt
//
LOCUS       DAFKMX010000035        25773 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFKMX010000035 DAFKMX010000000
VERSION     DAFKMX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.035"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(262..2481)
                     /locus_tag="LZB08_001204"
     CDS             complement(262..2481)
                     /locus_tag="LZB08_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBM7314715.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(2745..3842)
                     /locus_tag="LZB08_001205"
     CDS             complement(2745..3842)
                     /locus_tag="LZB08_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314716.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPVIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(4158..5351)
                     /locus_tag="LZB08_001206"
     CDS             complement(4158..5351)
                     /locus_tag="LZB08_001206"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBM7314717.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(5488..5591)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5780..6289
                     /gene="yyaC"
                     /locus_tag="LZB08_001207"
     CDS             5780..6289
                     /gene="yyaC"
                     /locus_tag="LZB08_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBM7314718.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(6400..6828)
                     /gene="fabZ"
                     /locus_tag="LZB08_001208"
     CDS             complement(6400..6828)
                     /gene="fabZ"
                     /locus_tag="LZB08_001208"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBM7314719.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(6844..7863)
                     /locus_tag="LZB08_001209"
     CDS             complement(6844..7863)
                     /locus_tag="LZB08_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBM7314720.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(8060..8320)
                     /gene="spoIIID"
                     /locus_tag="LZB08_001210"
     CDS             complement(8060..8320)
                     /gene="spoIIID"
                     /locus_tag="LZB08_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBM7314721.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(8418..9086)
                     /locus_tag="LZB08_001211"
     CDS             complement(8418..9086)
                     /locus_tag="LZB08_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBM7314722.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(9152..10216)
                     /gene="spoIID"
                     /locus_tag="LZB08_001212"
     CDS             complement(9152..10216)
                     /gene="spoIID"
                     /locus_tag="LZB08_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBM7314723.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(10303..11556)
                     /gene="murA"
                     /locus_tag="LZB08_001213"
     CDS             complement(10303..11556)
                     /gene="murA"
                     /locus_tag="LZB08_001213"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBM7314724.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIASTLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(11577..12314)
                     /locus_tag="LZB08_001214"
     CDS             complement(11577..12314)
                     /locus_tag="LZB08_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBM7314725.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(12742..14037)
                     /locus_tag="LZB08_001215"
     CDS             complement(12742..14037)
                     /locus_tag="LZB08_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBM7314726.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(14489..16321)
                     /gene="glmS"
                     /locus_tag="LZB08_001216"
     CDS             complement(14489..16321)
                     /gene="glmS"
                     /locus_tag="LZB08_001216"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBM7314727.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(16588..17934)
                     /locus_tag="LZB08_001217"
     CDS             complement(16588..17934)
                     /locus_tag="LZB08_001217"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBM7314728.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAILEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(18104..18661)
                     /locus_tag="LZB08_001218"
     CDS             complement(18104..18661)
                     /locus_tag="LZB08_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBM7314729.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(18662..19414)
                     /locus_tag="LZB08_001219"
     CDS             complement(18662..19414)
                     /locus_tag="LZB08_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBM7314730.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(19414..20493)
                     /locus_tag="LZB08_001220"
     CDS             complement(19414..20493)
                     /locus_tag="LZB08_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBM7314731.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(20522..20737)
                     /locus_tag="LZB08_001221"
     CDS             complement(20522..20737)
                     /locus_tag="LZB08_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7314732.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(20780..21457)
                     /locus_tag="LZB08_001222"
     CDS             complement(20780..21457)
                     /locus_tag="LZB08_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7314733.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(21447..22526)
                     /gene="buk"
                     /locus_tag="LZB08_001223"
     CDS             complement(21447..22526)
                     /gene="buk"
                     /locus_tag="LZB08_001223"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBM7314734.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(22548..23450)
                     /gene="ptb"
                     /locus_tag="LZB08_001224"
     CDS             complement(22548..23450)
                     /gene="ptb"
                     /locus_tag="LZB08_001224"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBM7314735.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(23754..24935)
                     /locus_tag="LZB08_001225"
     CDS             complement(23754..24935)
                     /locus_tag="LZB08_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314736.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(24932..25720)
                     /locus_tag="LZB08_001226"
     CDS             complement(24932..25720)
                     /locus_tag="LZB08_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBM7314737.1"
                     /translation="MSIYDLIDISIVAYIFYKIFMFIKDTRAEQVFKGIIFLLLATQL
                     SNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGRAKFNLFGKNVNTSEETL
                     NRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTIIDAEISRQLLINIFIPNT
                     PLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGIGVSEVSDCITLIVSEET
                     GGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGGIFK"
BASE COUNT         7669 a         4992 c         2824 g        10288 t
ORIGIN      
        1 ctttattaaa acaataacta cactcttcaa taaattcttt ttcaattgaa tgacgtatta
       61 ttggttttcc acacttcatg caaccatctt gtataaaatt catttcttta aaacagcttt
      121 tacatattga ataagtattt gttttcttta tggatttatc acaaattatg caacttatat
      181 tctcaggata tataaaatcc aaacatttat taatcatatt tttaaaattc ttaataaaat
      241 tatcattgca tattttcata tttattctat caacaaccct tcttgtttaa atttatttag
      301 cttatatcct aaatttgaat acctttgatt aactctatta ttttttatca tatattcaag
      361 atatttcaca tctccaacta atacaactaa tttttttgct cttgttactg ctgtatataa
      421 taaattacgg ctaagtaaca taggaggagc ccacgctatt ggcaatacta caactggaaa
      481 ttcacttccc tggcttttat gtattgttgt acaaaaacta tggtcaattt catctaattc
      541 atcatatagg tatgatacaa ttttagtctg gtcaaataat atataaatag ttttcttatc
      601 tttatctatg tgataaacat atcctatatc tccattatag ataccttctc cactttcttt
      661 ttggtcttct gtttcccatt tttttgtata attatttttt acttgcatta ccttatcgcc
      721 aactctaaat aatcttttag aaaaactttc ttcaacttta aatttttctt ttttgttaag
      781 atacttctgt aactctatat tgagattagt aactcccaat tcaccttttc tcattgaaga
      841 taaaacttgt atatctttta atttatcaac tttataaaat ttaggtaatc tttcattaac
      901 caatcctatt atttcattta aaatttcttc attagttgat tttcttataa aaaagaaatc
      961 tttacctttt gtatttagat aaagtggttc tccattgttt atcttatggg catttacaat
     1021 tatcatactt tcttgcgctt gtctaaatat ttcatttaat ttaactacat ttataatatt
     1081 agaattaatc atatccttaa gtacatttcc agcacctaca gaaggtaatt ggtcactatc
     1141 tccaactaaa attactcttg ttcctaactt tatagctcta agtaaattgt acattaagat
     1201 aatgtctacc attgatacct catcaacaat tattacatct gaatttattg ggtcttcttc
     1261 atccttaaaa aaagttaaat catcatctgt tgaaaatccc atttctagca acctatgtat
     1321 agtttttgct tctttattag aagtttcact cattctcttt gctgctcttc ccgttggagc
     1381 tgccaaagtc acacttttac cattattttc aaatatatct atgatagcat taatggtagt
     1441 tgttttacca gttcctggtc caccagttat aattaatact ccactattaa cagattcttt
     1501 gactgctaaa atttgttttt ctgctaactt aattttctta tcttcttcta aaacattaat
     1561 ctcgttatct atatctattt ttaagtcttc aaattcatac tgtgataatt ttactatttg
     1621 actacatact ccattttcag ctaaatagta tggcataagg tatataagat tttcattgtt
     1681 tactttctct atatgtattt tttgattata aactagcatc attacacact cttttattat
     1741 ttctccattt agctccaata atttttccga atcttgtatt agaacatgct ctggtaaata
     1801 tgtatgccca ttacttaaag atttatttaa agtgtataag attccctgac aaattctatc
     1861 tttagagttt ttatctatac caattttact tgctatacta tcagctactt taaatccaat
     1921 tcctcttata tcttcagcaa gttggtatgg gtttttattt ataacttcta ttgcactgct
     1981 tttatatttt ttatatattt ttaggcagta atttggtgtt attccaaaag gagatagctg
     2041 tattattata tttctcaatt ctctgtcttc ttcataactt tttactattt gttttacttt
     2101 tttactccct ataccttcta cttcttgtag tttttctggt gaattttgta tcacctctaa
     2161 tgtatctaca ccaaacttat caattattct ttttgccatt ttttcaccaa taccatgtat
     2221 cattccagaa gataagtaaa cataaattcc ttctaaggat gatggtgtta ctggcataaa
     2281 actattcact tcaaattgag tcccataaat cttatgattt acccactttc cttcaacttc
     2341 tatgctttct ccaattgcca aagtaggcat acatcctaca accacaattt catcattttc
     2401 atttaccagg tgagctattg tataaccatt atcttcgttt ttaaagacta tttcacttat
     2461 cattccttct aatttttcca tttttatctc ctaaaaatac taataatcgc tccatttaaa
     2521 tgttagtata attttatata ttaaaaccat gataatcgta gaaaagtttt atatttttat
     2581 acttaacgta atttccaact attatacatt ttatcagaaa caaaaaaaat ctgcatccat
     2641 aaaatatggt accatgaaat acaggtacca tatttaaata gctattttat tttaattact
     2701 ctatatttag agtataaatt aaatttcata agtatattac agtactaact tatttcagat
     2761 ttattctcct tgtacccctc agttttagta attcttctat aaagcataca tagtaaaatt
     2821 aaaattccca tgtaacctgt atatggatat agtgtaccta ctaacttatc aaatggcagt
     2881 aaacctccta tacaagctaa tatacttacc actattgtta taattttaaa cttagggtgg
     2941 tcatcttcta caaatcggtt tgttactgac cataacaatg gtacagctgt agtgtatatt
     3001 cctagtaaaa gtactactga gaataaaata cctattactg gagatatctt atcagctaag
     3061 taaagtgctg gtatctcttt tgtgtatata tttcctatgt ctgaaagtaa tgccaaattc
     3121 atcattatag ctgctgccat aagtgctact ccacctacta taccacccca tacagcatct
     3181 ttcttattta ctgcagaagc tcccattcct gtcaagaatg ttattactat tattacattg
     3241 taacaagtat aaagtactcc cgagaaatat ccatttgatg tagcagtttt aatatttaaa
     3301 ctatcaacca tatttccagc atttgataaa gcatcaatat tgctaaataa actaattgct
     3361 cctactaaaa gtgtaaatat tattattata ggtcctatat tacctagtat tttagaaagc
     3421 cttgtcaaac ctaatgaaac agttattagg gaaacgattg ccataccaat cctacctaca
     3481 tatggattta atccatagta ctctgataat gtagctcctg ctcctgcaat cattattact
     3541 aatgttccaa ataaaaacaa tggaccaaac cattcaaaaa atgttccaag atattttcca
     3601 caatatactt gataaatttt tattggctcc ttaagtttca attctcttcc cttatctata
     3661 acttctgctc caaaccatga aaatagtacc atagagatta ttccacctat aatacccgca
     3721 tatccataaa acgtaaaaaa ttgcagtatt tcttgaccag ttgcaaatcc agaacctatt
     3781 acagtagcaa cataagctcc agcaaaactt ataaccgttt taatatttac tttgcctgac
     3841 atacactact cccccccaat taaaattatt tttaaagtgt aagtttatta agattatatt
     3901 agactcatta ttgtgatatt tcaatatatt tttaattatt taacattagt taacacattc
     3961 ttaaccatcc tttaacatat atcccataat atgtataaat ttagtagagt attatataaa
     4021 atgtattgac cagattaaat actgtttatt cattatatga tgataaatta acatttatta
     4081 aaaatattta actcataata aaaaagctgt tagaaagtaa tcgtaaaatt acattctaac
     4141 agctttttag ttaaaaatta ttcacctaaa gcatcttttc ttaactgctc aaccttgtca
     4201 gttctttccc aaggtaaatc tatatcagtt ctaccaaagt gaccataagc agcaaccttt
     4261 ttataaagag gctttcttaa atctaagtct cttattatcg ctcccggtct taaatcaaag
     4321 tgtttattta ctaattcaac aagtttttct tctgaaacct tgcctgtccc aaaagtatct
     4381 ataaatatag ataatggtct agctatacct atagcatatg caagttctat ctcacatttg
     4441 tctgcaagac cagccgcaac aatatttttt gcaacatatc tagctgcata agcagcagat
     4501 ctatcaactt ttgtagggtc ttttcctgag aaagctcctc caccatgtct agaatatcca
     4561 ccataagtat ctattattat ttttcttcct gtaagacctg tatctccttg tggtcctcct
     4621 ataacgaatc ttcctgttgg atttatataa attttagtct cttcatcaag taattctgct
     4681 ggtataactt ccttaataac atgattaatt aaatcttctc ttattttatc atttgatact
     4741 gtttcacaat gttgagctga tataagaact gtatgtactc ttacagcttt acttccttca
     4801 tattcaacag taacttgagt ttttccatct ggtcttaaat aatcaactaa acctgttttt
     4861 ctaacttctg taagtcttct tgataattta tgtgccaaag atatcggaag tggcattaat
     4921 tctggagtct cattacatgc aaatccaaac attatacctt ggtctccagc tccaactttt
     4981 tctatttctt cttctatctc ttctccagtc ttactttcta gaccttcatc aactcccata
     5041 gctatatctc cagattgttc atcaatagaa gttataactg cgcaagtatc acaatcaaat
     5101 ccatattttg ctcttgtata tccaatttct cttactgttt ctcttactaa tttaggaatg
     5161 tctacataag cagatgtact tatttctcct gctactaaaa ctaaaccagt agtaactgta
     5221 gtttcacaag ctactctaga ttgtgggtct ttttctaata atgcatctaa tattgaatct
     5281 gatatttggt cacatatctt atcaggatgt ccttctgtaa ctgattctga cgtaaataaa
     5341 tgtcttgcca tttttattcc cccttattta ttcttaattt ttattatagg ttttacctta
     5401 tttttaattt tagaaattta ctcaaaataa aaaaacctct cctttataag aagaggttaa
     5461 taatcgtaat tacatactaa ttaactgacc tcatctttca agacttttgt cttgtaggaa
     5521 ttagcacctt ttcccatttg ggtggttgct gggcttcaca gggcctatcc ctcagcctct
     5581 cttgataagg taatcaagat ttaagttttg ttttacttta ctagcttact atttttttaa
     5641 aattttgtca atatttttag tcatattttt tctattcctt tttttgtaag acaaataaaa
     5701 ttttctatct atctgaaatt taaagttagg tctttttaat gctttttatt catatatttt
     5761 tttgaagggg gtgttcctat tgtatttagc taaagtaaac tacagcgatg atgatgttat
     5821 acagatgcta agttcagttc taaaaactat aattaatgaa aatacgattg ttgtttgcat
     5881 aggaacagac agggctatag gtgatacttt aggcccttta gttggaacaa tacttaagaa
     5941 cagtaacttt aaataccccg tttacggtac actagataac cctattcacg ccttaaatat
     6001 atatgagtct ttagatacta taaaaaatac acatatacaa ggtaacttct tggctataga
     6061 tgcttgcttg gggtcgcaga gtaacatagg taatatacag attagagaag gacctatcct
     6121 gcccggaaaa ggtgttggca aaaaactacc tcaaatagga aattattcta tagtaggaat
     6181 tgtagataaa attgatgaaa ataataaact ttcatttaat aatatacgcc ttagctttat
     6241 attagattta gctgaaacaa tagccttggc tttattagta tccacctaat ctaaatataa
     6301 actaaatgat aaaattcttt atataaaagg gtagatattt tagagtatct gattaaatac
     6361 tccaaattat ctaccttttt acttttatat ttcaagtttt tatttttcta ctaaagcaaa
     6421 cattaattca cctttacaga caagttcttc acctacatat gccttagcat ctgccttagc
     6481 tatatttttt ctaagagttg tcatttcaac ttccatagtt aatgtatctc ctggtcttac
     6541 ttctctcttg attctaactt tatctatacc tgcaaataca cctagttttc ctttgttttc
     6601 ctctacagac atcattgcca ctccacatac ttgtgctaga gcttctacta ttagtactcc
     6661 tggcatcaaa ggatattctg gaaagtgacc ttggaaaaat ggctcattaa ctgttacatt
     6721 ctttatacca acagctcttt tcccaacttc tagctctgta atcttgtcta ctaataaaaa
     6781 tggatatcta tgtggtatta atttttttat ttcatctata tttaacattc taaactctcc
     6841 taattatttt cttttgaaag acccaccagt ttcaagtaag tctattgaac ctaaagctat
     6901 tcctgtacct tttgcaacac aagataatgg ttcatctgct attgtaacag ttattcctgt
     6961 cctctgttct attatcttat ctaatccata taataatgaa cctcctccag tcattattat
     7021 accgacatct cctatatctg ctgctaactc aggaggcgtt ttttccaaaa cagcatgagt
     7081 agcaactact atttgctcaa cacattctct aagtgcttcc aacatttctg ttgaatttat
     7141 agttatagaa ctaggaagtc ctgtaattaa atttcttcct gttatcttca tatatttttc
     7201 ttcttctctc ttaaatgcag aacctatttc aaacttgatt ttctctgctg ttctttctcc
     7261 tataagaaga ttgtgttgtt ttttcatata tttaactata gcatcatcaa atctatctcc
     7321 accaaccttt atagattcac ttacaacagc tcctcctaga gatattacag ctatatctac
     7381 agtacctcca ccaatatcta taatcatatt accattaggc tgtgatatgt ctacaccagc
     7441 tcctatggca gctgctattg gttcttctat gatatatact tctctagctc cagcttgtct
     7501 cgtagcatct tctactgctc ttttttccac ttctgtgaca cctgttggta cacataccat
     7561 aattcttggc ataaaaaatc ttccaaatcc ctttttatca actatcttat ctataaaaga
     7621 ttttaacatc ttctcagtta catcataatc tgaaattaca ccatctctta aaggtcttat
     7681 agcgactata ttacctggtg ttctccctat catcttttta gcttcttctc ccacagctaa
     7741 aacagaatct gttcttttgt ctatcgccac aacagatggt tcttctaaaa cgattccctt
     7801 accactaaca taaacaagca catttgcagt acctaaatct atgcctatat cagctccagc
     7861 cattttttca actcctttat ttataatatt agattatatt ataagcctat tttaatactc
     7921 ctataataaa gcatatacaa aaatctcgct ttaatcaaga gaaaataaat cttttgagag
     7981 tattattatt ttgctattta taattactta atatatttct ttttataatt taattcaata
     8041 aaaaaagtag gtttttaacc tacatttttt tatgttcctt ctcatatttt aatttcgttg
     8101 ctaatccccc tctaatatgt ctctctgatt tatttttgtc cagcacattt ttaacttctt
     8161 tggcaaggga tggatttatt tccttcaatc tttctgtaac atccttatga attgtacttt
     8221 tacttactcc aaatgtcttg gcggtttgtc ttactgtagt gtttttttca agtatatatt
     8281 ttgctaccac tatggccctt tcctctatat gagatctcaa agattacccc cctcacataa
     8341 tagctttaat gatatatatg taaaaacacc aaaaaatata acaagcttaa ccaaatatga
     8401 ttaaacttgt tatactatta cttaattaga ctcattgggt ctatagattt ttctccacta
     8461 tacgcctcta aatgcacatg tataccttct tcactttcta tttgagaagt actacctaca
     8521 gtacctagag attgtccttc tgttaccttc tgttctttct taacactaac atttttatct
     8581 agatttgaat atactactac aatgttattg tcacttttta ttttgactga ttgtccatat
     8641 tcttcatcat caaatacatc tactactatt ccgtttaata gagattttac ttctttgcct
     8701 ttagtacaac taatatctac acctttatga gtttcccaaa catcaagtgt tttagaataa
     8761 ctaggctctt tttctgaata tcctcttatt actttatctc caatgtaatt taattttgtt
     8821 gtactcgatt tattttcctc ttttgactta gctttttcta agttttgttt tgaatccgtt
     8881 gcagtaggga tagcatcttt cttgtctttt tcaattaaat gtatttcctc ttcactatct
     8941 ttattagcat tttccataat tcctttatta gaagccaatt tatctacatt attatttgta
     9001 aaccaaactc cacctactgc taataaacaa acacatacaa acaaagataa ataaaaacca
     9061 tctttttcta acagcttttt cttcattttg cacctccaaa tatatttcta catttggatt
     9121 attgacgtgt ttttggcatt ttatacaatt attagtatat atcttttatt tttgtatctg
     9181 tgtagtaatg tgataatata tcataatatt tatagccctc ttctgccatt ccttctgctc
     9241 cccactggct cataccaaca ccatgaccat atccttttac cacgaaatct atgtatcctt
     9301 ctccaaattt tatgtcaaag tttgcagagt tcaatttaaa aacagttcta atgtcttttc
     9361 cagtcaattc tttatttcct aattttattt tttctacagt tccggcatca cttcttttag
     9421 ttattgaaac ttgtttactt aaattattca catctatcac aatagtgcta tatgcccttc
     9481 taagactttt aacaaaatct gtgttactta ttttaagtgt tgacgcaaat ttaggggaat
     9541 acttatcata aggactttcc acagacttta aataaggata ttttgcagaa aatacttctt
     9601 cactattttc tgtttttcct gaagatgtag aaaaataaag aggaagtatc gccttatcat
     9661 tataagttat tatttgacct ttagttcctc taactgcttc ttggattttg gaatatttat
     9721 tcttaatcca ttcttcacca tttaattttt taagagtatc ataactctta tattcttgac
     9781 aatgtttata atcagtacat actactgcat ttttatgttt actagattta ccatgttcct
     9841 gtttatatac tacatatgtt ctagctgcta cagattgagc ttttaaagct tctatatcaa
     9901 actctgaaga catctcacca gctagtactc cacataaata gttctccatg tccatctttt
     9961 cggttttccc tgttatatga ttatatacat tgataattgg tgctttttta tttacagttt
    10021 catagttcac tgtatttttt tcttctttat tacctttgtc ttcaatatca ctttttttaa
    10081 tagttttgtt tatagatact gggctctctg gtttttcagt taattctaca tttttataag
    10141 aaactaaagt tataagtgat ggaactaaca cagagcaagt cacaaatcct aacaaaacaa
    10201 ccaatgggtt cttcatatac tctcctcctt agcttttaaa accttgtatt taagtatatg
    10261 aacaacccta ttaatttata actttcttct ataaaccaat tattaatctt ctactatttc
    10321 gatttgacca cctagtgctg ttatcttctt atctatatca acataacctc tttggatatg
    10381 gtatatttct cctatttgag tttctccttc tgctataaga ccgcataaaa taagggctgc
    10441 ccctgctctt aaatcagtag cgtttactgc tgcaccgtgt agctcatcta caccattaac
    10501 aaccgcactt cttccatcta tttttatatt agcacccatt ctattaaatt cagcaacatg
    10561 cataaatctg ttttcaaaaa cagtttctat aactacacca gttccattgg ctacagtaag
    10621 catagccata aattgtgctt gaacatctgt aggaaatcct ggatgtggta aagtttttat
    10681 atctattggt tttaatactt ttggacctac aactcttaca gaattgtcca tttctgttat
    10741 ttcacaacca gcttctctta atttagctac aactggtttt aaatgctcca ttaaaacatt
    10801 ttcaacagtt atatcacctt ttgtcatagc tgctgcaacc atataagttg ctgcttctat
    10861 tctgtctgga attacattat gttctgctcc ttttaactct ttaacacctt ttatttttat
    10921 agtattagta cctgcacctt taacatctgc tcccatttcg ttcaagaagt ttgctaaatc
    10981 tactatttct ggctcttctg ctgcattctc tattattgta gtcccttctg ctaaagaagc
    11041 tgccatcatt atgttttcag ttgctccaac agatgggaaa tctaaatata atttatttcc
    11101 tactaacttt tctgttgctg cttcaacaaa accatggtcc atttctatct ttgcacctaa
    11161 agctttaaat ccttttaaat gtaaatctat aggtcttgtt cctatagcac atcctcctgg
    11221 catagaaatt ttcgtactat taaatctagc aagcaatggt cccataacca aaaaagaagc
    11281 tctcatcttt cttacaagct catatggagc ttcacaagtt tttatattgc ttgcatcaac
    11341 agtaagtgta ttttctttat attcaacttc tgctcctaca tgtcttaata aatctgatat
    11401 aacatgaaca tctcttaaat ttggtactcc atttaatgtt gattttccgt ttgctaataa
    11461 agtcgatgct attattggta aaactgcatt ttttgcacca tctattttaa cactaccttt
    11521 aagtggatta ctctttttta ctattatctt agccatattt tttgatctcc tcataattaa
    11581 taatctaatt ttataattgg tgtggcaata tatatgagtg tttttccgtc atcctcatta
    11641 tatttaattc ctatatttaa attaattttt tcccctaaat attctaattc ataatcattt
    11701 aatactttac tatatgctgt tatagataaa aagtttacgt ctttaacttc atctatttgc
    11761 ttagcactca tattatataa tattttttgt aaaatatcat catatttgtt gttttgtaac
    11821 ctttttgtgt attctccaac aatacaagca gacacatcta cttgagatga atatttattt
    11881 aacacatttt ctacgttact ataaataacc tctttatgtt tatatacttt gttatccaat
    11941 atgtcaacta ctatgtaact ttctttgtta tttttattta ttccttttat agaaatttct
    12001 ttatcgctac cttttatagt agcacataat tgtattttat cgccagaccc tttttgattc
    12061 caatttattc cttttttatc caactttaga ttgttaatta tttctataaa aatattatga
    12121 aattctttat ttgataaatt atgatttata acagaattaa ttttaacatt ataaaattta
    12181 aaatttgctt ctgatatttc aagagcttta cttaatctat tgtatctagt atttatactt
    12241 gtatctgcat aagatattgt cattcctata atcattaata taaaaaagct tatagctaat
    12301 tttacttttt tcatagtaaa aaatccccct taaatatgtt tttctgtata atagaattat
    12361 taacaaattt aagagagata tactttctca atatgtattt ttatacattt ttgtattttt
    12421 catctaataa tctctttcaa actcttcaat ctcaattttc tctatccttc tcctggactt
    12481 gccaatttct tatatgtatc aaatacatct tgcattaatt cttatatatt ctcttctgta
    12541 atataaatta cttagtctat acttaaatct ataatctatt catattttac caacaaaata
    12601 aacagataat acttttttat caatttccat agctccaaat gaacatacct aagtacactt
    12661 cccaatacac aatttacaca ctcttcctaa taaatcaaaa acacctttgt tttaaacaac
    12721 tctattgcac tttcaaaaac tttatttgtg ttctcccact tcaattaaat ttgaattatc
    12781 aagactccag cccatccatc catcactata aagggaggta ttttccactc ctatgacatt
    12841 tgcatatgtt aatacttcag cagcacgcca tccacttcca cacataaata taagctgttt
    12901 attcacatct atattttcct tgtcccacat ttctaaaatc tcatatttat ttctcattgt
    12961 attatcaata ttacgatatt cttctagtga aacactatca ctacctgaat gtccatataa
    13021 agctcctggt atacgccctt ttttatcata atatgtatat ccagatactt ttcctatatg
    13081 ttcatcccaa gtacggttat ctactagtat aaacttattt ttttctttta gtttttgtct
    13141 taattcagat gtagtaacaa ttaattgtga gttaactggt atatctgctc caaaattagt
    13201 acatgaatgt tttggattac taataaattc taattcatat cctgctgata accatgagtt
    13261 tgttccacca ttcaaaactc ttacatcttt tactcctata tacctcaata taacagctag
    13321 tctataagat gccataggtg tactacttga aactattaca gtatcatcct ttgttaatcc
    13381 atagtctaaa gcaaacttag ttaagttatc atcattatca agcatccatg ttggaggagg
    13441 ttctataata tcagtattta catgaacact tgtaggtata tgcccttttg tatacgattc
    13501 ttctccccaa ctcgcttcaa tcatttttat attctttgaa tcttcaaatg tctcaggaat
    13561 ttttccatct aatatatctt ttattataaa agcaggaact atcatttgat aatttttata
    13621 tctttccatt ggaagatttt cgtcgtctgc ccattgttta atatcatatt tatatagata
    13681 tttatatcct tttttagata aataatctgc tacaaccaaa gcatcttttc catttgcatc
    13741 atacaatact atatttttat ctaaatcaat tcttttagtt ttcaatgctt gttctaacac
    13801 ttcatctttt ctatctgagt atacacttaa ccaattagca gaaaaatcta ctgctccttt
    13861 tatatgtcca cctcttttta ctccatctaa cttccagcca ttataagcat catttaagcg
    13921 agtatcaact ataacccaag cgttatcttg cactttttct tgtaaaactt cagtagatat
    13981 tgccttaaga ttaacaaaat aatcttcttt cttttctttt ttaaaatcat ttgtcataat
    14041 ttatactccc aatttaaaaa aatttttatt tgaaattatt ttactaaaat aaagtaattt
    14101 tcttatactc tattagcttc attatattct tttataaata gttccttata ttcttatttt
    14161 aaactactca ctttatctta tcacaaggtt actaatacct tgtattttaa tttttctcat
    14221 aagctattat tttttatgtc tgatattgac tatatgttta ttaaatactc actatatttt
    14281 atatgaacta tatttatcgt tccctatacc tggtatggtc tatatattta tcattttaca
    14341 gatgatttaa tatcaatctt tttataaaat ttattatagc aaaaacaaaa caaatttaat
    14401 atttatctaa aaaactcatc ctataaaata caaataacag gatgagttct tttcaataaa
    14461 tattatctat ttaattttta atctgttttt attcaactgt tactgattta gctagatttc
    14521 ttggcttatc tacatcgcat cctcttagta cagagacatg ataagcaagt aattgcattg
    14581 gtacaactgc taatattggt gataaaatat catctgaatt tggtatatag attattttat
    14641 cagcagcttt ctcaacatcc ttattatggc tttgagctat tgctactaca taagctcctc
    14701 ttgctcttac ttcttccata ttagaaacca ttttttcaaa taacttttct tgagtagcta
    14761 tggcaattac aggagttcct ttttctatta aagctattgt tccatgcttt agctctcctg
    14821 ctgcaaaagc ttctgcatgt atatatgaaa tctcttttaa tttcaatgaa ccttccattg
    14881 ctaagctata gtctatacct cttcctaaat agaatgcatg ttcagaacta acaactgttt
    14941 tagcaacttc ttttatatat tcttcattgt ctagtatttc ttgtattttt gatggtattt
    15001 ctttcatctt agaaatcata ctatcataaa attctctagt tattgttcct tttttgatag
    15061 caaagtctaa agctatcatg tataaagaag ttatttgagt agtatatgcc ttagttgatg
    15121 caactgctac ttcaggtcct gcccaagtat agaatacatc atctgattct cttgctatag
    15181 atgaacctac aacgttagtt attgaaagta ttcttgctcc tttagccttt gaatctctta
    15241 atactgctag agtatctgct gtctcaccag attgactaac taatattact aatgaatttt
    15301 catctacaaa tgggtcgcta tatctaaatt ctgatgctat atcagttatt actggtatat
    15361 ttacaaattt ttctatggca tacttaccaa gtaatccagc attatatgct gttccacaag
    15421 ccactatata aactttattt attttttcta agtcttcttt agaaatattt atactgtcta
    15481 gaattatatt tccattatca tcaagtcttc tttctaaagt ttctcttact cctgttggtt
    15541 gctcatgaat ttcttttgac ataaagtagt catatccacc tttagacgca gcttctacat
    15601 cccaagttac atgaaatact tctttttcaa caagatttct attagagtca taaactgtta
    15661 cattttcgtc ttttagatga actacttctc cattttctaa gaaataaaca tctctagtat
    15721 attttaataa tgcaggtata tctgatgcta taaagttttc accttcacca acaccaacta
    15781 caagaggact atcttttctt actgcaacta attcatttcc atgttcttta catataacac
    15841 ctaatgcata agctcctctt aattttgata tagttttgta tacagcatct aataaattac
    15901 cctcatagta tttatctact aaatgtgcta ttacctctgt atctgtttga gattcaaact
    15961 taacaccttc agaaattaat tcttctttta tttccatata gttttctatt atcccattat
    16021 gaacaactgc tattgttttg gcttgattaa aatgaggatg tgagtttaca tcagatggtt
    16081 ctccatgagt tgcccatctc gtatgcccta tacctaaatt tccatcaata ggattctttt
    16141 ctaagtcttc tgcaagtaca gaaagtctac ctttaaactt tctaatatta agttcttttt
    16201 cattagaact attaactgct actcctgctg agtcataacc tctgtactct agcttagaaa
    16261 gcccttctac tataacttct gctgcctttc tacttcctaa atatccaact attccacaca
    16321 ttatttgtgt cctcctaaaa atattattta tatattttaa gagttaggtc atataagttt
    16381 actttggctt ccctttgtcc ataggattgc tcctattatt taaagaagtc tttcgatggt
    16441 aaacacaccg cagagcatcc gccgacaatt cgattaactc tgacctcgtc aacttagttc
    16501 ctatattact caataattat gtctataact aagttctggc gcttagaaag gtgtcctcag
    16561 tatttatatc tgagaatacc ctttaaatta cgataatttt tcttgtataa gattagctaa
    16621 gtttgttgct aattctttta tttgaccttc ctctttaccc tctaacataa ctctaactaa
    16681 tggctcagtt cctgatggtc ttatcaaaac tctaccattt ccgtctaaaa tagattctat
    16741 tctttctatc tcagttttta tttctggata ttccatatat ttgttcttat tttcattttt
    16801 tattgttgca tttactaata cttgtgggta ttggctcatt attgatgcaa tttctgataa
    16861 aggtttcttt tcttgcaaga ttatatttgc aagtattaga gaactaagca ctccatctcc
    16921 agtagtgttg taatctaaaa atatcatatg tccagattgc tctccaccaa gattatatcc
    16981 atttttaacc atatcttcta aaacatatct gtctccaact gctgtagtag ataaatttat
    17041 tccattttcc ttagcagcta ttgtcaaccc tatattactc ataactgtaa ctaccaaagt
    17101 atcttgtgcc aatttattat ttttctttaa atttaaggca ctcaatatca ttatatggtc
    17161 tccatcaaca atttgaccct tttcatttac tgcaattaat ctatcagcat ctccatcata
    17221 tgcaagacct aagtcagcat tatgttccaa aatagccctt tgaagttgtt ctggatgtgt
    17281 agagccacat ttatagttta tattatttcc atctggtgaa ctatttattg aaattacact
    17341 tgctcctagt tcatcaaaca ctattggtgc cactttatat gacgcaccat tagcacagtc
    17401 caaaactact tttaatccct taaagtcagt gcttattatt gattttaggt agtctacata
    17461 gtctctttgt gcacaatgtt catgtaattt tcttcctaca ttttcaccaa ttggaagaca
    17521 atcaattttg tctatatcgt ctatatattc ttctattttc aattcaattt catcatctaa
    17581 tttatatcca ttcttattaa agaatttgat tccattatat tcaacaggat tgtgagatgc
    17641 agatataacc acaccacaat cagcaccata ttttctagtt aaatatgcca ctgcaggtgt
    17701 tggtattatt ccaacagtaa taacatcaca tccaacagac ataagtcctg ctattaaaga
    17761 tgcttccaac atatcacctg atattctagt atccttacct actactactt ttactctgtg
    17821 gtctccttga gctaagacaa atccaccagc tctaccaagt ttatatgcta aatcacatgt
    17881 tagttctgta tttgctactc ctctaactcc atctgttcca aaatactttc tcactaaaga
    17941 ttctcctttc ataattgaaa agcttgtaat ttgtattata tttttatagt tcataaaata
    18001 tcattatagg cttaatttca gtcataaaaa tattttaata cttatcagca aaaaagacaa
    18061 tagaaataat tctattgtct tttttttgac atctttttct ttattagtta gttgctttag
    18121 aacatgtagc atccatacct ttttgtaaag cttctctgtt taatggaacg aatttttctt
    18181 ttctaggtcc gaaaactttt ttgaatgctt ctagtacaga ttctatatct acagtatcag
    18241 atacttttaa gtaagcgcct aacattatca tgttagcaac tttatcattt cctaattcag
    18301 ctgctaattc atttgctggt atataataaa catctacatc tgttctatca actttttctt
    18361 ttattaaaga actatttact attatttttc caccaggaat aacatcatct ttgaattttt
    18421 caaatgctgg tatatttaat aatatagctg cagttgcatc atctgttata agtggagaac
    18481 caactggctc attagataca gttactgcac agtttgctgt acctccacgc atttctggtc
    18541 cataagatgg taaccaagaa acttcttttc cttctagcat tcctgcataa gtaagtaatt
    18601 gtcccataga cataacacct tggcctccaa agcctgcaca tattactcta gctgtagaca
    18661 tttacttcac ctcctcaact tcaacatttt tgaaatttcc taatggatag tatggtatca
    18721 tgttgtctct taaccattgt aaagcatcgt ttggagctag tccccagttt gtaggacaag
    18781 tagataatac ttctactatt ccaaatccta atcctgcttg ttgaacttgg aatgctttct
    18841 ttatagcttt tttagcttgt ctaacatgag ctggagtatc aactgctact ctttctacaa
    18901 atacagctcc tgttaatgta gctaacattt cacttactct tattggataa ccttgagttt
    18961 cagcatttct tcctgattga gcagtagtag ctctttgtcc aactaatgta gttggagcca
    19021 tttgtccacc agtcatacca taagttgtat tgtttacaaa tatagtagtt attttttctc
    19081 ctctagcagc tgcatgaact atttcagcag taccaattga agctaagtct ccatcacctt
    19141 gataagtgaa tactgtattt tcaggatgaa ctctttttat tcctgtagca gcagctggcg
    19201 ctctaccatg agcagcttct tgagtgtcac agttaaaata tttatatcct aaaactgaac
    19261 atccaactgg aacaacacct acagcatcac ctaatactcc tagttcttct aatacttcac
    19321 caactaatct atggattata ccatgtgtac atcctggaca gtagtgagtt tgagtatctt
    19381 gtaatccttc agttttctta aatacaacag ccattatcta gcacctcctg caaccaattc
    19441 tccggcaata atttttttag ctttttcaac tatttctgct ggtgttggag tcattcctcc
    19501 tggtcttcca tggaagtaaa ctggtaattt accttctact gccattttaa catcttccac
    19561 catttgtccc atactcattt caacagtcaa tatgtttcta gcattaggaa tttggttgaa
    19621 tgcttcgaat gggaatggcc ataatacttt aggtcttata agacctgctt ttattccttc
    19681 ttctcttaat atatcaattg cgctttttac tactcttgaa gtagttccat aagctgcaaa
    19741 tacaaattca gcatcttctg ttttgtacat ttcgtattgt acttcattct tttccattgc
    19801 atcaaatttt tcttgtaaat gccaacaatg gtcttctagt acttctggct ctaagtatag
    19861 agagtttatt acattaggtt ttctttttcc tttagttcca acagttgccc aatctttagg
    19921 aggcaattct cttttcttct caggagctct aaattcaact ggctccatca tttgacctat
    19981 cataccatct gcaactacca taactggtga tctgtaataa tctgcaacgt caaaagcttc
    20041 cattatcata tcaacagctt cttgaactgt agctggtgcg aatactggag ttctgtagtc
    20101 tccatttcct ccacctcttg tagacataaa gtagtcagct tgtgaaggtt gtatacttcc
    20161 aagtcctgga ccacctctca ttacattaag tacaacacaa ggaacttctg ctcctactgc
    20221 atatgttata ccttcttgct ttaaagcaac tcctggtgaa gatgaagaag tcataactct
    20281 tgctcctgca cctgctccac cataaaccat gtttatcgca gaaacttctg attcagcttg
    20341 aacgaacgct ccacctattt taggtaactc tcttgataaa tattctggta attcactttg
    20401 tggagttatt gggtaaccga agaaatattt gcaaccagct tctatagctg cttttccaaa
    20461 tgcttcgtta cctttcataa gtatcttagc cattaaaatt cccccttaaa tattaattaa
    20521 attaatctct ttctactgtt ataactacat ctggacacat agttgcacaa cttgcacatc
    20581 ctatgcattt ttccatttca aaaacagttg ctgggttata cccttttttg tttattacat
    20641 ttgagtctaa ttgtatgatt tttactggac atgcttcaac acacaatcca caacctttac
    20701 aacgttcttg attaaaagat acttttcctt tagccattta cctaagcctc cttaaacttt
    20761 ctatatattt gagctcaaac tacatccagt cttctctcat atataatttt ataggaaaaa
    20821 tttctccttc taaatctttt ggtaactgtt caacaagatt ttctaaacat gcaacatatc
    20881 ttatcggtat attacatttt tcagaaactt cttttgcaac tttctgacct cttaaaacat
    20941 catcaatagt agtaaaccta ataagatgag tattattaat tagacctgtt acctttaatt
    21001 tagaagattt ttctatttct tttatatatt ggataacttc ttcagaagtt tgagttttct
    21061 ccctatttgc atttattaca aaaagcatat cataatcgcc ttcttttaac aaatgactaa
    21121 atcttcctat aaccctagca cctacattat ctccacctaa atctataaca gaattgtatg
    21181 aatggtctac cataggagac attacctctg ctgatactgc tggtaaatct aatgttggag
    21241 cattaattga gctatctatt ggttgtatac ctaaggattt cattaactct ttcttttctc
    21301 tagttctaaa gtatacattt accacatcta aatcggctat agcaactttg ccagaaacca
    21361 tatctcttaa cttaacaaca tagttcatcg caaattcact tttaccactt ccatagtgac
    21421 cagttatgat tcttattctt ttatcattag ttatcataaa cttgagcctc ttcttcacca
    21481 gttaaaactc taagtccacc ttgagctaat gcaatcattt catcttcacc tggataaact
    21541 tttacatctg ctataaattt aactctatct gcaatcattt ctgtaaacat ttttgaatat
    21601 gcgattccac cagttaataa tattgctttt acatctccct taagaactgc agcactagct
    21661 cctatttctt tagagatttg atatgccata gcttcatata ctaatttagc tttttcatca
    21721 ccagcttcaa ttctttcttc aacttctcta gcatcattag tgtttaagta tgcaactaat
    21781 ccgccattac cttttatttt ctttttaatt tcatcttgag tatattttcc actaaagcac
    21841 atttttacta atgcacctac tggtagtcca ccacttcttt ctggagagaa aggtccttct
    21901 ccatctaatg cgtttgcaac atctactatt ttaccatttt tatgagctcc aacagaaact
    21961 cctccaccca tgtgtgcaac tataagattt atatcttcat atttcttgtt tatttctcta
    22021 gcatatcttc ttgctattgc cttttgattt aaagcatgta ctacacttgc tctacttatt
    22081 tcaggcatac cagaaattct agcaacatct tctaattcat ctacaacaac agggtctact
    22141 atgtatgaag gaacatttac ttcttcacct atttgttttg ctattattcc acctaggttt
    22201 gaagcgtgtt ctcctaaaac tcccactttt aaatcttcaa tcatagcagc acttactgaa
    22261 taagtaccac cttttatagg tttaagaagt cctcctctac ctactacagc atctaattca
    22321 gatgttttta ctccaccttc ttttagagct tcttctatta cttgtttacg aaattcaaat
    22381 tggtcagaca ccttctcata ttttcctatt tcttctgaag aatgtcttaa agttttttca
    22441 aatactaaat cctcattatc aaatacagct attttagttg atgtcgaacc aggatttatt
    22501 gttaagattt taaatatttt gctcatttgc ctatttcccc cttaaaatta tgcctttgct
    22561 gccattaaaa cacctaaagc tattgagttt agtttagttt cttcactgtc tgctctagaa
    22621 gttaatatta ttggtgcttt agccccaact ataactcctg cattttttga ttttgagaag
    22681 aataccaaag ctttatataa tatgttacca ccttcaatat ctggggctaa taatatatca
    22741 gctcttcctg ctacaggatg atttatacct ttatgtttag ctgcttctaa agatactgca
    22801 ttatcaattg caaaaggtcc accaaccata caacctttga tttctcctct ttcatacatt
    22861 tcttctagtt ctttagcttc aactgtatct ttcatttttg gatttacttt ttcttttgcg
    22921 catattgcag caacttttgg ttcacttata tctaatgaat gtgctactgt gcaagcattt
    22981 tctatgattt gctttttagt atttgtatca ggagctaagt tcatagctgc gtcagttacg
    23041 aaaaataatc tatcatatcc ctctacatca aatactgcta cgtgacttaa tacatttcca
    23101 gttctaagac ctacttcttt atttaaaact gcttttagta ttattgatgt gtctactaag
    23161 cctttcatta ccatgtcggc ttttccttgt gaaactaatt caacagattt tagagatgct
    23221 tctgctaaat cttttatatc tatcagttca taattttcga tatccatgtc tatgcttttt
    23281 gcaatttctt tagtcttttc tatatctcct actaaaatgg catttgctat tttttctttt
    23341 ctagccattt caactgccat taaaacttct ttatcttggc aacatgctac tgatatagtt
    23401 ttaggtcctc tttcttttgc aaacttaatt acttcttcaa aacttctcat gcatttccac
    23461 ctcatcttga attttttgaa atagaacttt ttatatctca attaaattat aactcgttca
    23521 gcaacgtttt cctagagata attgaaatat ttaattcaaa atatttattt aaatctattt
    23581 atcagtcttt tttcatatat ctagcgcttt ttagatttaa caaatatctc attgtcctta
    23641 ttttataata taactaaaat aaacaagatg caacaaaaac ataaaattaa caaacttaat
    23701 tttataggtt tttttgtcgc atcttgtaac aactaaatat tcgaaatatt tgttcattcc
    23761 acttcaacaa tattaggttc tatatttatt tttttaaatt gatatgttgt ttcatatttt
    23821 attttatatt tactatcttc ctcaggacct tgtgataaat caatatataa tgaaatatct
    23881 tttttattta acttactcaa atcctccgtt gactctacaa caacttttat atctacatca
    23941 gaaattacat ttggattatc catatttgtt gggatatttc ttaattcaat atctcccgca
    24001 ttatataaaa attctgcact caattgtttt gtatcattta atttaattgt tatagagcta
    24061 tcctcaagag ttataccctc tggaatttct aagctaactt ctttttcttc accattagcc
    24121 aaatcagcta agtcaatagg ttttgtctta atataactta ttttgtctat atcttctttc
    24181 tttcctttta ttgtaacttg ttccttactt aatttatagt ttttcagtac atcattatct
    24241 ttattatcat ttagacttac tcttattggg acaatttttt ccttcaatag ttctatcttt
    24301 gcagtcacaa aggatgaact taatgttact ccttcaacct tagttccatc actatttaat
    24361 ggtattaatt gcaatttttt agaaaaatca tctgtctcat taccaacatc tagattagct
    24421 cttacacttt tgacttcttt aactagagtt cttggacctg atacatcgac aaattcttta
    24481 ctaagttcaa ttttatttat atttcttttt gaatttccac ttacttctac ctttatatct
    24541 cttttttctt ttatgttttt ttcaagattt actactaaaa catcatgctt aaattctaga
    24601 gtaacacctt gaggaatatt tgcttttaaa tatacttcct tctgtccttg tattgggtct
    24661 tcaatggttc catatacatg tatatcttct tttttaacac tttgtacctt tgataaacta
    24721 ccttttatat atacatctgc tgttaatttt tcgtctggat atataacaag atttttatcc
    24781 tttaaatcac tcatattact tatagataca ggtatatctt caaacccttt agtttcttca
    24841 gggtcaacta cagccataac atacatccat aatgcaattg cacttaaaaa tgatattatt
    24901 tttattttgg tattattttt tagtctattt atcatttaaa tataccacct ttgaaaaaac
    24961 ttcttgtttc tgtatttgtt tttaaattac tacgtaatat attcatcatt ctttctctag
    25021 aaatatctct atataactta cctgctttag caattgatac gccacctgtt tcctctgata
    25081 caatcaaagt tatacagtca gacacctcac ttacccctat tcctgctcta tgtctagtgc
    25141 ccaaatcttt acttaaatcc ttactttctg tcaatggcaa aaaacatgca gctgctttta
    25201 ccttagaatc tctaattaca acagctccat cgtgtaatgg tgtatttggt ataaatatat
    25261 ttattaatag ttgcctagaa atttcagcat caataatagt tcctgtattt attatatcac
    25321 ttattctagt ttctctctcc attatgataa gagctcctat tttttgtcta gaaagtgaat
    25381 ataaagcttc tacaatttct tctatatttc tattgagagt ttcttcagaa gtatttacat
    25441 tctttccaaa taaattaaac tttgctcttc ctatatgttc aagtcctgct ctaaattctg
    25501 gttgaaaaat tataagagca gctataactc cataatctaa agctttaaga gatatccaat
    25561 aaacggtatg caatttaaag gtgttactaa gttgagttgc taataaaaga aatattatac
    25621 ctttaaacac ttgttctgct cttgtatctt ttataaacat aaatattttg taaaaaatat
    25681 acgctactat agatatgtca attaaatcat atatactcat tcttaatatt atattaaaaa
    25741 tccatttaaa aaagaattta tatctaacac agt
//
LOCUS       DAFKMX010000036        25610 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFKMX010000036 DAFKMX010000000
VERSION     DAFKMX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.036"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            259..1125
                     /locus_tag="LZB08_001227"
     CDS             259..1125
                     /locus_tag="LZB08_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7314738.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1367..2176
                     /locus_tag="LZB08_001228"
     CDS             1367..2176
                     /locus_tag="LZB08_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314739.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2320..3384
                     /locus_tag="LZB08_001229"
     CDS             2320..3384
                     /locus_tag="LZB08_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBM7314740.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3412..4131
                     /locus_tag="LZB08_001230"
     CDS             3412..4131
                     /locus_tag="LZB08_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7314741.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4155..4739
                     /locus_tag="LZB08_001231"
     CDS             4155..4739
                     /locus_tag="LZB08_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBM7314742.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            4741..6090
                     /gene="pabB"
                     /locus_tag="LZB08_001232"
     CDS             4741..6090
                     /gene="pabB"
                     /locus_tag="LZB08_001232"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBM7314743.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDVTDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6080..6823
                     /locus_tag="LZB08_001233"
     CDS             6080..6823
                     /locus_tag="LZB08_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBM7314744.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYTKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            6857..7558
                     /locus_tag="LZB08_001234"
     CDS             6857..7558
                     /locus_tag="LZB08_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314745.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7584..8156
                     /gene="folE"
                     /locus_tag="LZB08_001235"
     CDS             7584..8156
                     /gene="folE"
                     /locus_tag="LZB08_001235"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBM7314746.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8220..9011
                     /gene="folP"
                     /locus_tag="LZB08_001236"
     CDS             8220..9011
                     /gene="folP"
                     /locus_tag="LZB08_001236"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBM7314747.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9022..9387
                     /gene="folB"
                     /locus_tag="LZB08_001237"
     CDS             9022..9387
                     /gene="folB"
                     /locus_tag="LZB08_001237"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBM7314748.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9380..9886
                     /gene="folK"
                     /locus_tag="LZB08_001238"
     CDS             9380..9886
                     /gene="folK"
                     /locus_tag="LZB08_001238"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBM7314749.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            9867..10652
                     /gene="aroF"
                     /locus_tag="LZB08_001239"
     CDS             9867..10652
                     /gene="aroF"
                     /locus_tag="LZB08_001239"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBM7314750.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            10907..12697
                     /locus_tag="LZB08_001240"
     CDS             10907..12697
                     /locus_tag="LZB08_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBM7314751.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12715..13881
                     /gene="rpoD"
                     /locus_tag="LZB08_001241"
     CDS             12715..13881
                     /gene="rpoD"
                     /locus_tag="LZB08_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBM7314752.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14046..14735
                     /locus_tag="LZB08_001242"
     CDS             14046..14735
                     /locus_tag="LZB08_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBM7314753.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14749..15846
                     /locus_tag="LZB08_001243"
     CDS             14749..15846
                     /locus_tag="LZB08_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBM7314754.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            15909..16481
                     /locus_tag="LZB08_001244"
     CDS             15909..16481
                     /locus_tag="LZB08_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBM7314755.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16541..17008)
                     /locus_tag="LZB08_001245"
     CDS             complement(16541..17008)
                     /locus_tag="LZB08_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBM7314756.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17394..17870)
                     /locus_tag="LZB08_001246"
     CDS             complement(17394..17870)
                     /locus_tag="LZB08_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBM7314757.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(17990..19081)
                     /locus_tag="LZB08_001247"
     CDS             complement(17990..19081)
                     /locus_tag="LZB08_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBM7314758.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19074..19463)
                     /locus_tag="LZB08_001248"
     CDS             complement(19074..19463)
                     /locus_tag="LZB08_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7314759.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19655..20101
                     /locus_tag="LZB08_001249"
     CDS             19655..20101
                     /locus_tag="LZB08_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314760.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20365..21090
                     /locus_tag="LZB08_001250"
     CDS             20365..21090
                     /locus_tag="LZB08_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314761.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21077..22321
                     /locus_tag="LZB08_001251"
     CDS             21077..22321
                     /locus_tag="LZB08_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314762.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKQFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            22401..23078
                     /locus_tag="LZB08_001252"
     CDS             22401..23078
                     /locus_tag="LZB08_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314763.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23079..>25610
                     /locus_tag="LZB08_001253"
     CDS             23079..>25610
                     /locus_tag="LZB08_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314764.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENI"
BASE COUNT        10455 a         2556 c         4554 g         8045 t
ORIGIN      
        1 aagcagtatc atattatgct agaaaaagtt tcatttaagg cataaatgag atgatttgaa
       61 cgtttttaaa gattagaata taaaagattg cactagacat atatgaaaaa gataatataa
      121 tagatgatgt aatagatgac agttcaagaa attataaaaa aaatttattt gaaatattat
      181 ctattacatc aggaataata tatgtaagag taagctttta aaattctaaa gatttagaaa
      241 gattgaaggg agaaagaaat gagtgaagtt aagaataccc tacaaaaaca tccagtttta
      301 agatattcaa caatgctatt tgggatgttg atgacatcta taggaataaa tgggttctta
      361 agaccagcac atctgcttag tggaggtgct acaggtatag caacatctat aaactatttg
      421 acaaatataa atgtaggttt attaacattt ttaatcaata ttccaatatt tatcttaggg
      481 ttcatatacc ttgaaaaaga gttttgtatt tcaagtttgg taaatatgat tgtattttca
      541 ttattattag gagcaactca ggaaataagt aatattatac ctatacatga tatattgctt
      601 caaagtgtat atggtggtat attatctggt ttaggagtcg gagttgtatt tagaactagg
      661 tcttctcaag gtggtacaga tataatagct gcaatactaa aaattaagaa gaatatagaa
      721 atgaaagaca ctgctttagc aataaatggt ttgatagtac ttacaggaag ttttttattt
      781 gggttggatt tagcattata cactcttata ggtctatttc taaatgcgta ttcaatgagt
      841 tttattaaag atgcaatgaa ttaccaaaaa tctgttatgg taatgtcaaa tgaagtcgat
      901 ttaatagcag aagatataat gaagagttta gttagaggtg taactttttt agatgcagaa
      961 ggtgcataca ctcatcaaaa aaagaagatt atatatacaa tagtttcatc aaatgagata
     1021 ccaaagataa aagatatagc tttgaagtat gataaaaagg cctttatatc tgtaaatgat
     1081 gttacagaag taaagggaag agggtttaaa gcaaaagatt tatagaaaat tttacaagct
     1141 gggagtttat aatgtaaatt tgtatattat atctctcagc ttatttaaat ttagtatacg
     1201 aaaataaagg ttttattaat gatttgtgta tcactttaaa agatatcatt ttaaaattaa
     1261 aattgaaaaa attacttaat atgtagaaaa catgaccact attaggatat aataaattta
     1321 atagatttag taaaatatct aggaataacg ttggaggaat ggcattatga atttatctaa
     1381 agaatatgtt aatttgtatg ataattattt gaaatatata aatgaagtaa acaaagagat
     1441 tagaagtata aagaacatga agaaacgtgt tttaaaaagg ggcgattttg ctccggaaaa
     1501 tgaagtatta gaattggaag gaaaaacaat acgcgatata gatgatgtcg atacaaaaaa
     1561 accaaaaaat aaaatctata aattttcaaa tggggatgta tatgtaggga aattcttaga
     1621 gggaaagatg gaggggaaag gctcatatac tttttttgta gatgaaggaa cagttatgga
     1681 gtacatagga gaatttaaag aagataaaaa aaatggaaaa ggaaacttta tattttcaaa
     1741 tggaaatgaa tatataggtt attttgagga agatatgatg agcggtattg gaaatatgtt
     1801 atatagtagt aaagatgaat atattggaac ttggaaaaac ggaaaaaaag atggaaaagg
     1861 tatttataag tggagtgatg gatgtatata tgcaggtgaa tttaaaggtg gaaagatgga
     1921 agggtacggt atttgctata atagtaaagg ggaagtactt tatgaaggag aatggaagaa
     1981 taatttgatt catggtaagg gaacttatat atgggaaaaa ggtaaaaagt atataggtga
     2041 atttatgcat ggtaaaaaac atgggcaagg aactttttat ctaaataatg aattggttta
     2101 tgaagggact tggaaatttg ataagccatc tatatttgat agaacattag atgaaatatt
     2161 ttccttgaga ctgtagcttt atctaattag tagataaagc ttttttctaa tacttactgt
     2221 aaataggtat gaatgatatt gattagtata cactatcaat attaacttaa taaatgcaat
     2281 attaaaaaat tttgattgat tttaagaagg agagatatat tgaagagatt aattgcttta
     2341 gtgttagtag tacttattgt tttattttat agtcctgaat tattaaaaga aggatatgat
     2401 ttaaaagaaa agttttttca aagtgaaata tggaatgaat ttttagacaa aataaatata
     2461 aatgataatt ctaagaaaga taaaaaagaa aaaagtcaaa atttacaatc aaataatact
     2521 gaaggggaga acttaggaga gtcagatata aagaattttg aaaaaataag cttaggagat
     2581 aatttaagct atgttttatc aagtatcggc aaacctggta gaattgatat tagtgaatat
     2641 ggttttgatt ggtatgtata taatcaatat ggaaaagaat ttgcaatggt tggacttgaa
     2701 aatgatgaag tagtagcact ttatagtaat tctattaatt catgtgaaaa tcaagatata
     2761 aaactaaatc aagatagaca aacagtgaga actaagatta caccattaaa atataagaga
     2821 aaaggaaata ccagatatat aataaactca gaaaatcaat atgatataat aagtaaggaa
     2881 ggtaagtata ttacaatttt ctacgatatt catgaagaaa atagagtttg ttcatattta
     2941 ataattgata aaagcactga agatgagttt aaaaatttat atccagatga tagcgaagaa
     3001 cttaaaaaat gttttgaact tgaagttata gaccttgtaa atagtgttag aaatcaaaga
     3061 gggttaaact cattgagata cagtgaacag gcaactttga gttcaagaaa acatagtgaa
     3121 gatatgagag ataataattt ttttgaccat gtaaataaga agaatgaaac tccatttgac
     3181 agaatgaaaa gagaaggtat agtatatact tcagctggag aaaacatagc agcaggacag
     3241 attaatgcaa tatatgcgca tgaagcttgg atgaactctg aaggacatag aaaaaatatt
     3301 ttggggaact ataataatat tggagttgga gtaatatttg gaggaagcta taaaacatat
     3361 tacactcaaa atttttataa ataactaaaa tatttgaaag gaagttaaaa aatgacatca
     3421 tggaaaaaaa agacagtata taagtgctta atagcagtag tcttgttttg tggaatagtg
     3481 ttaatatcta attttagtaa agtttctgct ttgatgatgg atactaatgg aaatgttttg
     3541 attaaacatg gttcaaggga aaaaaagcta atagcaatta cctttgatga tggtcctcat
     3601 cctaaagaaa ctagtcaagt tttagatgta ttaaagaaat acaatgtaaa agctactttt
     3661 tttatagctg gaaaacatgc aaaatggtat aaagaaccat tagtaagagc aagtaaagaa
     3721 ggtcacgaga taggaaatca tactttcaat catcctgata taagtaattt aagttctagt
     3781 cagatagaag aggagattgt taagtgtgaa gacattttga aggaagtaac tggaaaaaaa
     3841 ccgacattat ttagaccacc atttggaagt tatagagaaa aagaattaat agaaattgca
     3901 aaaaagcatg actataaagt tgttttatgg acaggtgtag atgtgaaaga ttggaaaaat
     3961 ccaggagcta atagcattgc agataaaata attaataaag tacaaaatgg agatataata
     4021 cttttgcatg attatgcaac taatgataca gttgaagctt tagatatgtt tattccaaaa
     4081 atgattgaaa aaggttttaa atttgtaaca gtttctgagt tgataaaata atttttaggg
     4141 gggagattta cagtatgatt cttatgatag acaattatga ttcttttgta tacaacctag
     4201 tccaatatat agaagaatta ggggaagcag ttgtagtaaa aagaaataat gaaatcaaaa
     4261 taagtgacat tgaagaacta aatccagagg taattgtgtt atctccagga ccgtgttctc
     4321 caaaagaagc tggtatttgc atagatattg ttgagcattt taaggggaaa aaacctatac
     4381 taggaatttg tttaggtcac caaactattg gacatgtttt tggaggagat attataaaag
     4441 cacaacagcc tgtgcatgga aaagtatata gtatcaacca tactaataaa ggagttttta
     4501 ggggacttaa aaatccttta aatgtcacta ggtatcattc actaatagtt gactccaata
     4561 cagttccaaa agaattagaa attacagcaa ttactgataa aggggaaata atgggaatta
     4621 ggcataagaa atatttaata gaaggagtac agtttcatcc agaagctatt ttgtcagagt
     4681 atggtcatga aatgcttaaa aattttataa cagaagctag agaaagggta catatgtaat
     4741 atgataagag agataaatac taaattaaat tcttttgaga tatttactat ttttagaaat
     4801 gaacatgaca gttttatatt agacagtgct atggataaag aaaaattggg aagatattct
     4861 tttataagta gccaaccatt taaggtatta aagtataaag atactgatga aaatccttta
     4921 gaagttttaa aagaagaact acataagtat agggttgtaa atgatactaa tcttccattt
     4981 gtaggaggtg ctgtgggata tttatcttat gatttaggta attacataga aaatttacct
     5041 agaactgctg tagatgatat tgagatgcct gatatgtatt ttggatttta taatcatgta
     5101 atagttatag accatcttgt gcaaaaaact tatatagcta ctccaaacat agatatcgaa
     5161 ttagaagaaa agataataga tgatatagaa caaagaatat taaaagaaga gaaaaaaggt
     5221 atagatagca tatgttatga agaaaaagaa gtaacaccta taagacttaa atctaatttt
     5281 acaaaggaag aatttaaaaa tgcagttcaa agtgttagag agtacataag acaaggagat
     5341 atatatcaag ctaatttaac acaaaggttc agtggagaaa cagaacttac aagctttgaa
     5401 ttgtataggg acttaagaag atttagccca gcaccatttg gggcattctt aaactttgaa
     5461 gatacacata tattatcaaa ttctccagaa agatttataa gatgtgttaa taagagaata
     5521 gaaactagac caataaaagg tactcgtcca agagggaaag ataaagaaga agatttaaga
     5581 cttcaacaag aacttagaaa tagtgaaaaa gatagagctg aactacttat gatagtagat
     5641 ttagaaagaa atgatattgg cagaatatct aaaactggaa gtgttaaagt tccagagcta
     5701 tttgtaattg aaccatatgc aaatgtaaat cacttagttt ccacagttgt aggtgagtta
     5761 aaagatgata aagatgttac tgatgtaata aaagcaactt ttccaggggg gtctataaca
     5821 ggagcaccta agattagagc aatggaaatt atagacgaat tagagccaac gcaaagaaat
     5881 gtatatactg gttcaatagg ttatataggt tttaatggtg atatggattt taatatagca
     5941 attagaacaa taatcaaaaa tgacaaaaaa gtctactttc aagttggagg aggaatgact
     6001 tgggattctg acccagatga agaatatcaa gaaacacttg ataaagctaa atccataatg
     6061 aaggctttga ggggatatta tgaagaataa tgtaggtttt gatagtagtt taagtaaatt
     6121 tggaattggt ttgtttgaaa ctattagagt taaaaatgga atagcagtag atttagatat
     6181 ccatatagaa agaatgctaa gttcaataaa ttgtttggat ttagatataa actatacaaa
     6241 agatttcttg ataaatgaaa ttgttacata tataaaaaaa gaaaatgtca ttaataaagc
     6301 acttagaatt actgtttttg atgaaggtta taatatatct attagggata ttccatataa
     6361 tcaagagacg tatgagaagg ggtttaaatt agctatatct ccaatagtta gggggaatag
     6421 tttaattcat agacataaga ctactaatta ttttgaaaac atatatacca aaaatatcgc
     6481 aaataagact ggttataatg atggtatatt tttaaattca gaaggtgtaa tattagaatg
     6541 ttctatgagc aatattttct ttataaaagg agaaagagtg tacactccta gtgatagatt
     6601 accgatatta aatggaataa taaaaaaaag gattatagag atttgtgacg aattacatat
     6661 agagttgata gaaaatgaaa ttaatatatc agaaattagt agttttgatt ttgtttttgt
     6721 cacaaatagt cttatgggcg ctataaaggt tacagaaatt gacaaaataa aatttaataa
     6781 agaaaatgtt gtttttgata aaatagtgaa gttattagaa taaattaaaa tattaacaat
     6841 ttgttggaga caaaagatga taggttgtat ttatgaagta tacaatgaag agatgactaa
     6901 aataatagaa aaccaaaaca ctttggctgt atatttagtg ggttctagta aagatgtaga
     6961 ttttactctt tatgatgtag tgataaacga tattgatgtg ttcgtttttg taaaagaagg
     7021 ggaaaaacaa gagagaatac tgtttaaaaa aaagggaata gaatttgatg taaattactt
     7081 ttcaaaagat ggtgtaaaaa aattattatc aaatagggaa tatttctttg tgaaagaaat
     7141 gaaagatgct aaagtactgt ttgataggga gaatatatca catattataa aagatataag
     7201 tagagatatt tacttagaag gacctagtaa aatgtcatta gaggaaaagt cttttataaa
     7261 gcaagatatt ggagcaaaaa tatcgaattt aaaaaataaa gaaaaatttg atgtttttga
     7321 atatcatttt ttgacgaatt tatatttaaa agatataata attgggtact ttaatataaa
     7381 tgataaatgg gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag aaaataatga
     7441 gctttttgaa ctagtgtcaa aagtaagtga aaattatgac tatcaaagac ttttagatgt
     7501 ttataattat atttttaaag aaattgagac aaaagaggtt ataaaactaa tcttctagtc
     7561 acaaatcaat ggggggagtt taaatgaata aagttgataa agaaaagata caacatgcag
     7621 taagagaaat actagaggca attggggaag acccagatag agaaggtcta atcgaaactc
     7681 caaatagagt tgctaggatg tatgaagaga tattctctgg tcttagtgaa gaaccaagag
     7741 accatttaaa agttcttttt gcagatgaaa aacatgaaga gttagtattg gtaaaagata
     7801 ttccatttta ttcatgttgt gagcatcatc tagttccttt ctttggaaaa gctcatattg
     7861 cttatttacc aaaaggagga agacttacag ggctttctaa acttgctaga gtaatagata
     7921 cattggcaaa aagaccacaa ttacaagaaa gaatcactaa aaatgctgca gatataataa
     7981 tggaagaatt acaaccttat ggtgtcttag tagtagtaga agctgaacat atgtgcatga
     8041 caatgagagg cgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg agaggaatat
     8101 ttgaaaagga cattgcgtct agagccgagg ctatgagttt gattactatg aagtagcaat
     8161 ttattataga tataatcatt tattgagata aaatttaatt taagggagtg tcattaattg
     8221 tgtttgatta tggtaaaaga acttatataa tgggaatatt aaatgtaact ccagatagtt
     8281 tttctgatgg aggagatttt aataacttgg acatagcaat acaacatgca aaagatatgg
     8341 ttgaccaagg tgcagatata atagacctag gaggagagtc tacacgccca ggtcatagct
     8401 atgtagactc agacgaagag ctaagaagag taatccctgt aataaaaaaa cttaaacagg
     8461 aattggatat accaatatca atagacactt ataaggcaga tgttgcagaa gaagcattaa
     8521 aattaggtgt cactatggta aatgatgttt ggggtcttag aaaagataaa aatatggctt
     8581 ctgtaatagg aaaatatgat gcagaagtgt gtataatgca taatcaagat gggacaaact
     8641 atgacaaaga tataatggag tcaataaaag acttttttaa ggtaagtata gaaatggcaa
     8701 tgagttgtgg tgtaaaaaaa gaaaaaatag tacttgaccc aggtgtagga tttggcaagg
     8761 attttgaaca aaatattgaa gtattgagaa gattaaatga attaaaagat ttagggtatc
     8821 ctattttgct tgggacatcc agaaaatcag taataggaaa agtacttcca gttgagccta
     8881 aaaaaagatt agaaggtaca atagctacta ctgttcttgg tataagagat ggcgtagata
     8941 tagttagagt acatgatgtt tatgaaaatt taatggcagc tagaatgact gatgcaatat
     9001 atagaaagta ggtataatat aatggataaa atattactta gtaatctagg attctatgga
     9061 tatcatggtg ttcttaaaga agaaaatttt ctaggtcaga aattctttgt cgacatggaa
     9121 ctttatattg attcaagaga agcaggtctt agtgacgata taaataaatc tgtaagctat
     9181 gccgaggttt acaatgttgt taaagatatt actgaaaata aacaatttaa tcttttggag
     9241 gctttagcgg aaaatattgc tgaagaagtc ttgaataaat ttatacttat aaatggagtt
     9301 atggtcagag ttagaaagcc tgaggctcct gtaaatggta tttatgacta ttttggtgtt
     9361 gagataagga gaacaagaga tgaataaggc ttatttggga ataggtacta acatgggtga
     9421 tagatttgat aatttatcaa gagcatgtga acttttgaaa aatagtgatt ctatatataa
     9481 agtaaaagaa tcaagcttat acgaaacaaa accatgggga tatacagaac aagcagattt
     9541 tctaaatatg tgtgtagaaa tagaaacaga gtttgaacct tatgagttgt tagaatattg
     9601 tcaagaaata gagagagaac tacacagaga gagaatagtt cattggggac caagaactat
     9661 agatgtagac atactatttt ttaatgatgt agtttccact gatgaaagac taacaatacc
     9721 tcatccaagg attcaagata gagctttcgt tttgatacca cttatggatt taaatgaaaa
     9781 acttataata aatgaaaaaa caataaaaga acatttaaac ctcctatctg ctgaagaaag
     9841 agaagaagta aaggagcttg taggttatga aagaaaacct atttaataat aattcaaaga
     9901 tagagataaa tacaaataac aaaaagataa ctgtaaaaaa agataaactt ataatagctg
     9961 gaccatgcgc aatagagagt tatgagcaac ttttggagac tgctaaattt gtaaaaagtc
    10021 agggagcaaa cattttaaga ggtggagcat acaagccaag aacatcgcca aattcatttc
    10081 aaggattaaa aaaagaaggt cttgaaatat taaaagcggt taaagacgaa gttggtatgg
    10141 ctgttattac agaacttatg gatgtaagag atatggacga actgtatagt ataagtgata
    10201 taattcaaat tgggtcaaga aatatgcaaa actttactct tttaagtgaa gtgggaaaac
    10261 aaaataaacc tgttatgtta aaaaggggaa tagcatccac aattactgaa tggataggtg
    10321 cttctgaata tatagcaata gaaggaaata gtaatattat aatgtgtgag agaggtataa
    10381 gaacgtataa tgattatact agaaatacat tagatttagc tgcagtaccc attattcaaa
    10441 aggaaacagg tcttcctgtt gtggtagacc caagccatgc cactggagtt agatatttgg
    10501 taaaacctat gtcactggca tcttttgcat gtggagcaga tggaataatg gtagaagtac
    10561 atcctgaccc agaaaatgct ttgtcagatg gtgctcaatc tctttgcttt aatgaatttg
    10621 aagatttaat gaaatcaatt aataattatt aaaagaggca agagcctctt ttttattcgt
    10681 aatcgttgct aaggtaaatt tataagttat aatttgatag ttagattatt taatataaat
    10741 aaagatttaa tattgataga attaatataa aaaataaaaa ttttttgatt aataagaagg
    10801 atatcaataa aaaaagtcga attgtgtata ttgagtgatt gacaaattta gaatatagtg
    10861 tagaatagaa atgttaacac aaaaaattac accagaaggt gataatatga atgatataaa
    10921 agacattatt gaagaaataa aagctagatg cgatatagca agtataatat ctgaatatat
    10981 gaatataaaa caatctggag ctaattacaa aggattatgt ccatttcatg gcgaaaagac
    11041 accttctttt tatataaata cgtcaaaaca gatttataag tgttttggtt gtggtgaagg
    11101 tggagacgta ataaattttg tcatgaaaat ggaaaatttg gattttatgg atgctgtaaa
    11161 aattttagca aataaatgcg gtatagaaat taacactaac atgaatgaag aaactagaat
    11221 taaaatagaa aaatctaaga aatttcaaga tattcatacg gaagcagcta gattttatct
    11281 ttcaaactta ttgggaagca aaaatcttgg gtatgaatat ttaagaataa gaggactaga
    11341 tgataaaatt attaagaaat ttggtttagg attttctttg gattcatgga attcacttat
    11401 gaatactcta atcagtaaag gatataaaaa acaagactta ttagagtgcg gtctaattgc
    11461 aaaaaataga gatggaacta attgctatga taaatttaga aatagagtta tgtttcctat
    11521 attcgattat agaggaaata taataggatt tgggggaaga gtattagacg attctttgcc
    11581 aaaatactta aattctccag acacattaat ttttaataaa aaacagaact tatacggatt
    11641 aaattttgct agaaaaaatt tagaaagtaa aactatagtc ttagttgaag gatatatgga
    11701 tttaatttcg ctttatcagt atggtataaa aaatgtagta gcaacccttg gaacagcctt
    11761 gactgaacaa caaggcattt taataaagag atatgcggac actgctatta tatcttatga
    11821 ttctgatgaa gcaggaataa aagcgacttt aagagctata gatatactta ctaaattagg
    11881 tatcaatgta aaagtgttag acttaaaaga tgctaaagac ccagatgaat ttgtaagaaa
    11941 atacggactt agtgattata aaaaagctat ggatgtttct actcactaca taaaatataa
    12001 aatagatcat cttaaaaaag aatttaacat tcaaaaagat gaagaacgag tgaagttcgc
    12061 aaaagaagct tctaaaatta taaaacaatt gacaagtcct gttgaaattg atttttatac
    12121 aaagtattta agcaatcaaa tagatattaa tgtcgagtct ataaagagag aagtttatgg
    12181 taaaaattac aataagccat ataataacaa aaatcaaaag aaaatagaag aaaaagtaat
    12241 tgaaaaagtc gaagtcagac aagacggaaa acaattagtt gaagaaactc taataaaaat
    12301 aatgttagaa gacaaaaaaa ttagagaaat agcgttactt aaagtagaag aaagtgattt
    12361 tttattgaaa gaaagtaaag aaattttaaa ttacatgatt aaaaatcaag aattggacaa
    12421 aataactatt gacaaattga aaagtttaaa catatccgaa gaatacttaa aagaattaaa
    12481 ttcaatttct ttgaatagta taaacttaga gaacacaaaa gaaatagaag gaataataac
    12541 aaatatcaga aaaaactcat tagaagagca aattaatagc ttgttgagag aacaacaaga
    12601 attagaaaat aataatgata tgaaagaggt agatggtaga gttatggaaa ttgctttaaa
    12661 aatagttgag ataaataaaa ttttaaagag cttgtagaga aaggaggttt gagtgtggaa
    12721 aataaatcga ataaaaaaga gttaaaaaag gttactgcta agacattaat agaaaaagga
    12781 aaaaagcaag gttctctgac acttgcagag ataatggaag ctttttcgga gactgaactt
    12841 gataaggatc aagtagaaaa tctttatgag actttaggaa atttgggaat agaaataaca
    12901 gaaacaaaaa actataaagc tgatatagat ttttcggttg ctgatgacga tttaagtata
    12961 ggccacttag atgaagatgc agaggcaatt tcacatgatg actcttctgc aatagaaata
    13021 gaaactgtgg atttatcttt accaaaaggg ataagtatag atgaccctgt aagaatgtac
    13081 ttaaaagaaa taggaaaaat tcctctactt aaaccacatg aagaagtaga atttgctaga
    13141 aggatgcacg aaggtgatga gatagcaaaa caaaggttag ttgaagcaaa cttaagacta
    13201 gttgtaagta tagcaaaaag atatgtagga agaggtatgc ttttcttgga tttaatacaa
    13261 gagggaaatt taggtcttat aaaagcagtc gaaaaatttg actatacaaa aggatataag
    13321 tttagtactt atgcaacatg gtggataaga caagctataa cacgtgctat tgcagaccaa
    13381 gctagaacta taagaatacc agttcatatg gtagagacta taaataagct aataagagta
    13441 tcaagacaac tacttcaaga acttggaaga gatccaaaac cagaagaaat tgcaaaagaa
    13501 atggaaatga cagaagataa agtaagagaa ataatgaaaa tagctcaaga ccctgtgtct
    13561 ttagaaacac caataggaga agaagaagat agccatttag gtgactttat tccagatgat
    13621 gatgctccag ccccagcaga ggcagcagca tattctttat taaaagaaca aatagaagat
    13681 gtacttggtt cattaaatga tagagaacaa aaagtattaa agcttagatt tggtcttgag
    13741 gatggtagag ccagaactct tgaggaagtt ggaaaagagt ttgatgtaac tagagagaga
    13801 ataagacaga tagaagcaaa agcactaaga aaactaagac atccaagtag atcaaaaaaa
    13861 cttagagatt atttagacta aaaagataca tcattgatgt ttttgtgata aatgccatga
    13921 gataagtgat atctcatggc tattttgtag taaagattta attttatttt tgtagttatt
    13981 tcagtctaat atctataact tggattaaat cctataaata aagggaattg aaaggagaat
    14041 tatatttgaa attaacagat agattattga aaatagcatc attagtgtca gatggtaaaa
    14101 aaatagctga tatagggacg gaccacggat acataccagt atatttgtta aaagagggaa
    14161 aagtaccatt tgcagtgcta gcggatgtta ataaaggacc tcttgacaat gcacataaag
    14221 aagtcataca gaacaatctt ttagataaag ttgatttacg attgggttct ggtatagaga
    14281 tacttgaaat aggggaagta gaagaagtga taattgctgg tatgggtgga atcttgataa
    14341 gtgagcttct cgaggctaaa aaagaagtag cacataatgt agaaaaatta atattacagc
    14401 caatgcaagc acaagaagaa cttagatatt accttttaaa caacggatat gaaattttag
    14461 aagaagtttt agtgagagaa gattttagaa tatatgagat aattgttgcc aaatatacag
    14521 gtaaaaatac tataatagaa gatgagatat attatgaggt tggaataaag cttttagaaa
    14581 acaaagattc tctttttaat gattttatag aaaaaaagat aaagacatat agttctatag
    14641 ttaataaatt agaagggaaa aatggggaag ctatagataa gaagagaaag gaaagcgaag
    14701 ttgcaataaa aaaacttgaa aatttaataa aataaatagg aggtatatat gctattaaag
    14761 agtctaacta gaaaaattga aaaaaagtat cctctaaact tagcagagga ttgggataat
    14821 gtaggtctta tagttggaga ttttgatatg gatgtaaaaa aggtattggt atctttagaa
    14881 gctaatgaag atgttattga tgaagctata tccaaaaata tagatttaat tgtgacacat
    14941 catcctttta tatttgggaa aattaataaa attaatagtg gagatttaaa aggcagactt
    15001 atccaaaaac ttattaaaaa tgatatatca ctttattcta tgcatacgaa ttttgatata
    15061 gcatttgatg gtctaaatga ctattttatg gaaattatgg aatttggtaa ttcaaaggtt
    15121 ttagatataa ctaaaagcga aaatttgtat aaattagcag tatacgttcc acataactat
    15181 tcagatgaac ttagaaaggt tttaagtaat tcaggagcag gtcatatagg gaattatagt
    15241 gattgtacgt tttcaataga aggtgaagga caatttaagc cattagaagg ctctaatccg
    15301 tttcttggaa gtgtaaatga tatagaaact gtaaatgaag ttaaaattga aactgtagta
    15361 ccacagaagc ttttaggtgg ggtaataagt tctatgttag atgcacatcc ttatgaagaa
    15421 gttgcatatg atttgtacaa gttagaaaat aaaggtgaga tatttggact aggtagaata
    15481 agtaaacttg ataaaagtat gacattagaa agtctttcaa ggaaaataaa agagaaatta
    15541 aatatgaaac acataagagt agttggaaac cttagtacag acataacgaa agtagccgtt
    15601 gtaacaggag ctggttcaga atttgtaaaa aaagctaaga gacaaggtgc agaagtacta
    15661 attacgggtg atgtaaagta tcatgaggca caagatgcac ttgatattgg aatgtgtata
    15721 gtggattgtg gtcattttga tacagaagat atattcaaaa atgttatgaa gagattttta
    15781 gatgaattta gtgaaattga agtaataaag agtaatgtat atttgaatcc atttagtact
    15841 atttagcagt gtttgataat aaaaatacta tataaaaaat aaatttgagt ttaattaggg
    15901 ggataatgat gaaagtagca gtaatttttc atagtgtttg tgggagtaca tatttattag
    15961 ctagagaata taaagaagcg cttgaagaaa tgaatatcga ggtagatata tttagagtaa
    16021 gtgacgaagt tgcaaaaaca cttcctcaat attatttgat aaattccaaa gaatacaaag
    16081 acgaatttga aagtataaat gtaattaaat ctggaaaaga aatattagat tatgatgcta
    16141 tatttatggg ttcaccaaca tattatggaa atgtatctgg accaatgaag atgtttatgg
    16201 atagtttttc tgatatttgg gtaggtgctc ctttgagtgg taaaatattt ggttgttttg
    16261 ctacagcagg ttcacaacat ggaggaggag aattagcttt gcaagctatg aatatatttg
    16321 cccaacatat gggaatgact cttttgtcag ttccatgttc agtaagagga ggttatccag
    16381 cgtatggaat acttcatata gcaggagata attctgatat aagaccaaat gatgatgcca
    16441 agataggtat aagagattac ctaaaaagac ttaacatata agaatatcat ataaaaatac
    16501 ctaaaagcga ctgtatatac ttttaggtat ttttacttaa ttataaacgt gaagctttac
    16561 cagaaatatg gtcaatttct attttaacaa cagttgttaa gttcatatcc ttctctatgt
    16621 acttcatgcc ttcttcaaag aatcctttag aatatttttt tattatttct acaagagcat
    16681 ttttcttttc ttcattttct acagtgcaag cttttccaaa aactatagca ctagaataag
    16741 tagtactaaa tttatctgga ataacacttt cattagcaac tactaaaaaa gaaactttgt
    16801 tgtttttcga tatattatct aacttatgcc catttcttgc acaatgaaag taaatagaat
    16861 cattaaagta tacataattt acagcaactc cataaggata accattttca gatatagtgg
    16921 aaaaagtacc aaattcacca ttttttaata cttcaacagt atcttctttt gtcatttctc
    16981 tttttttcaa tctcatttct ttaaacataa tatcatcctt tctaattttg atgcttaaat
    17041 taaattttag aacatttata aagattatgc tatgtttttt acaaatttta agaaaaaaat
    17101 atagtttaag ataaaaggta gtgaaagaga agctatttgg aggtttattt atgatattat
    17161 tgttagagta gtacaaataa tactaataaa tttaatagct ggtttcttgt tatttaaggt
    17221 agtaagcatg tctataagaa ggggaagtta tcgagggtag aagttgttgt ttataacgat
    17281 attataaaga aaatattatt atataaaata ataaatagta aagaggtatc aagttgaata
    17341 taatacttaa tacctcttca aaattaagtt ataaaagtgt tgtgggaatt gtcctataca
    17401 tggtaaggag ataataaaga atgtatcttt aaagtctcaa ctaaacagca aaatttatcc
    17461 aacaaacata caataaatgc ttctttgtat ctaggtggct taacagtaag tggccacatg
    17521 tgttttaaaa ttatgtccgt ttctttttca tttatttgaa aaaataaagt tgcatttttt
    17581 aatgcttctc tagggtgagt aaatccatgt aatccttttc tatcaccttt gtaatgccaa
    17641 tcataaagaa aaaagtcgtg tagtaaagct cctctaataa cactttgaat atcaacactt
    17701 aagtgcaatt ttttacataa tagataacta taatatgcta ctgacaaact atgttcaaga
    17761 catgaaacat tgccatgatg aggaaatgtt ctcataagtt gaactttttc agaagaaagt
    17821 atctcagaag cacattcatt aaaatatttg atttcagctt ttgacaacat tatgaaaatc
    17881 tccattctta gtaatatata aatactatac ttcaacgata atatgaaata tttcttatcc
    17941 tacaaaatat aatacccaat aatacaagta agataccttt tatggtattt taaagtaaga
    18001 gagattttat taggctaaac aatgaaaaaa ttaaaagtaa agctatagca atatatagta
    18061 tttttccaag aggattgcca aaatttatag tccatccaat accaaacctt ttattaacca
    18121 ttaatgatgg gtcatttgga ttattataca tgatgcctgc tatccaatac ttctcatctt
    18181 cttctggaga ataagatgag ttgaatttta aatttgggga attaatatat gttgctatta
    18241 aataaattat aactaaaagc gttgtaatat tcatagcagt tgatagtttt gtttttattg
    18301 ataaaagagt agtaaaaaac tgtgttgtca ttataatcat caatattaaa aacaaataac
    18361 caattttatt taaatattta atgttttcaa ctctactttc ttcaattcta tttgtatcta
    18421 tcttaatcct tgactttatg gatccaatgg aagttatata taataaaacc attaaacaaa
    18481 agtcagtacc gattaattta aatacattaa gaaatgattt atctataaaa acatcaggag
    18541 acccatttat gttccaatga gtggcaattg agtctggcaa ttgattatag ttaaatgcta
    18601 taaaaatact cattcccaaa gtaaatagta tcggtattaa atagattatt ttaaattttt
    18661 ttattatttt atttttctta tttataaaat ccatatctac tattaatttt gaatctatat
    18721 ctatatgacc taatttagca taaatttctg atttaagctt cttagctttt ttatgtgtat
    18781 gaatatatag tatactttca tataataaaa aacctataat accaataaaa taggaaagtt
    18841 ccaatttatt aaatgtaaaa accagaataa aaactaatat aaagtctata ataaaaccta
    18901 aagataatag ttttttaaat tttttatcta actttataaa gtaattataa tttttatgct
    18961 cttggtctat agaaactcca tagatgtgtt ttttacctac aaaatagggc ataaaataaa
    19021 atgtagcata cattaaaatt agaactggaa atattgtaaa tagacattca atcttactca
    19081 taatatttca cctcatactt gtcaaataaa cttgaactta gtgacaaaaa ttcttcttta
    19141 ctaatacctg ataaataaga ttttgccacc aataattcca agtcaagttt atttttttga
    19201 cgagttgtat tatctatttt cattggtagt tgagctacaa tagcaccttt tcttctatca
    19261 atatttaaga atccctcttc ttttaattca ttgtaggatt tgttcactgt atgtaaattt
    19321 acaccaatat tttcagccat acttcttaca gatggaagtg attcatttat tttcaattct
    19381 cctgaagcta tagccttagt tatttcttct ttaatttgta cataaataga tttatcacta
    19441 ttaaaatcta aatttaaaac cataaaaaac ctccattatg ctttataaat gaaaacgatt
    19501 gttatataca tagtataaca taaaaaataa taacaaacac aaaatataag tttatatttt
    19561 gtaaaaattg taatttaaaa aataacataa tttttattaa aataatacag atactattta
    19621 tagatagaaa taaaaattag aaaggagaaa aaatatgtta acatatttaa tgaaaagaca
    19681 aatgcataaa aaagatgatg gaatgagtaa accaatgatg attacaggag ctgtaattac
    19741 aggtgtagga gcatatgctg tatataaaat ggtaaaaagc cataaatcta attctcaaat
    19801 ggtcaataca aattattatg gaaatagcga ttatgatgat tataaatatg acgaatttga
    19861 ttatgactgt gattgtagag acacaaaaca agattatgaa tatgaagaat taaaaaaaaa
    19921 agtaaaagaa tttaattcaa gaagaataaa ttgtgaaaat tgtccacatg tttctcaaga
    19981 agaaatgatg caatatgtaa atagtgttaa agatgtaaag gaagagattg atttacatga
    20041 agatgctaaa aaagataata tttacaaaga agatgaatac aaaaaatatg aagaagaata
    20101 agtaatattt tgtataactg aacaaatagt aatgtcaaat tatttaatat ctagttttaa
    20161 tatgtaagtt gtaacttaac ttgaaagaat cagtatcatt ctttagatag ttaagtaaca
    20221 acttacattt tatttttata aattttaata gttctttgtg actattttgt aattttagaa
    20281 atattttagt atccagggta attgtgtata ataaaacttg tatagataga tagtatgaaa
    20341 ttaagcagat agtttaaaat taaaatgaaa agaaaggaaa tagttatgtt aaagttgttg
    20401 atagttgaag atgatagtac tatatctttt ggaataaagt atgctttaga acaagaagga
    20461 tttagtatag atatatcaaa ggatttatct agtggaaaag aaatgatttc ttctaatgaa
    20521 tatagcatga tactgttgga tgttacattg ccagatggaa ctggttatga gctttgtcaa
    20581 tatataagag gattctctca ggttcctata atattcttga ctgcttgtga tgaagaagta
    20641 aatattgtaa tgggtctcga tataggtgga gatgattata taacaaaacc ttttagaatt
    20701 agagaattaa tatccagaat aaaagctgtt ttaagaagaa aaggtaatac ttcagaagaa
    20761 aataagaaaa tacttaaatt tggagattta agtatatata cattagaagc tagagtatat
    20821 aaaaatgata aagaaatatt tctgacatca gttgaatata aacttctttt aattcttata
    20881 cagaataaaa atactgtttt aagtagaact caaatattgg aaaaactttg ggatgtaact
    20941 tatgattttg taaacgacaa tactcttact gtttatataa aaaggcttag agaaaaaata
    21001 ggtgatgatt tatgtgaacc aatctacata gaaactgtaa gggggattgg ttataaatgg
    21061 ataggaagtg aaaatagtgt ttctatataa tccagaagtg aagaagtttt taagtaagta
    21121 tgttactttg atgtttattg ttatcataat atctattgga tttagcataa tcaacgtgtc
    21181 tttaactaaa gagatggttg taagaaataa tcaagctata ataggaactt tatcaagcaa
    21241 gtatccaaac ttagaaagtc agatagtaga cataataact caagggaagt ctatggagaa
    21301 catagattat ggtcagaaaa tattaattaa gtataattat gataaatcta ttaggataaa
    21361 ctctgagcct attatttcta aattagtttt agagactatc aaaataaata taatattagt
    21421 atgtataata tttattttga tatttgtgtt ggttgttaga tattttaagt caatttacaa
    21481 tgatttatca gatatgacta agtatgttta ctatagttca gagggtaagc aatttgatat
    21541 gaaaaacaag aatcaagaag ggcagatagg tcttctaaaa actgaactct taaagatgac
    21601 gacaattctt aatgaaaagg ttgaactttt aaaaagagaa aagatatttt taaacaatac
    21661 aatttccgat atatcacatc aattaaaaac tcctatgact tctttaataa tgttgaatga
    21721 tttattatat aatgatgtac catacgaagt aaaaatcgat tttttaaata agataaaaaa
    21781 tcaattaaat agaatggatt ggttgattaa aagcatgctt aaattatcaa aggtagaagc
    21841 aaaagtgata aattttaaaa aagataaggt aaaatttagt gaactcatac atagagcaat
    21901 gctatctatg aaaataccta tggagattaa aaatcaaaaa ctgactatag agggaaatga
    21961 taatgtatct tatattggtg atattgattg gtccgtggaa gcattagtga atataatcaa
    22021 aaattgtgta gaacatactc ctgaatttgg aaatataatt ataacctata aagaaaatcc
    22081 attattttct gaactaataa taaaagatga gggtgaaggt atagataaaa aagatatacc
    22141 acatgtattt aaacgattct atagaggaag aagtagttca aaagaagata gtgtaggaat
    22201 aggtcttgca atgtcaaaat ctataataga aagccaaaat ggcgatatat atgtaaacag
    22261 cgaaaaaggt aagggtacag aatttcatat aatatttcat aaaatgtatg atagtgacta
    22321 atctgtaata tttaattcac tttatagtaa tagccaaaac ctaatatatg aagtatagaa
    22381 tataaatatt ggaggtagtt atggaaattt taagagtaga aaatctaact aaaagttatg
    22441 gtaaaaatga aacaaaagtt gatgctataa aaaatgtaag tttatcagtt gaaaaaggaa
    22501 cgtttatagc aataacaggt ccaagtggaa gtggtaaaag taccttatta catttattag
    22561 gaggagttga cagaccaact agtggtaagg tttatataaa tgatgttgat atatataatt
    22621 taaaaactaa ggatttagcg atatttagaa gaagaaatat aggtcttata tatcaatttt
    22681 acaacttaat tccagtctta aatgtgaagg aaaacatatt gcttccagca gagcttgaca
    22741 atagagatat tgatagagaa tattttgatg atttaatgaa aacattaggt ctaattgata
    22801 gagaaaagca ccttccaaac caattatcag gaggtcaaca acagagaacc tctataggac
    22861 gtgcattaat taatagacct tctataatat tagcagatga gccaacaggt aatttagata
    22921 gtaaaaattc aaaggaaata ttagaattgt taaaattgtc agtaaaaaaa tacaaccaga
    22981 cactaataat gataactcat gacaaaaata tggcacttca agctgataga attataagca
    23041 tagaagatgg aatcataaaa agtgatgagg tgatgtagat gaatttatat acatcactaa
    23101 ctattagata cttaaaacaa aataaaagaa gaaccattgt aaccataatt ggtataatac
    23161 tttcaacagc tttaatttgt ggtataggaa atatatttga aagttttatg gattatcaaa
    23221 tgagagaaac tataaagaat gatggaagtt tccatgttac attttatgat gtaaatagaa
    23281 aaaatgtgga atatgtaacg aaatcagctg aaatagaaaa acatgcattt accaaacaac
    23341 ttggatattc taaattagaa aatagtgaaa atgctatatt aagtataaag caatatgata
    23401 aaaatgcaat aaatggatat aaagtttcta taaaagaagg tagatttcca gctaaggaag
    23461 gagaaattgt tttaagtgaa agtattatta atcttataga tgataagtta aaaataggag
    23521 ataagataac tttaaaagta ggggatatgt ttgactcaag caagaaaaag attgatagta
    23581 tgacatttca tgaaggtgat tatatagcaa atgaaaaaga tagaactttt aaagttgttg
    23641 gtataattga aaaacctggg tttgaacgtt ataatggaat tgcaacagct atagcctact
    23701 ttaatcctat gaataattac aatgatacaa ttaatgtatc agtagcagta agaaatccta
    23761 aagatgtgta taagataagt aataaaataa gtaaaaatat atattcaaat aaagataaag
    23821 aagcagtttc tgatatgata aaatacaatg aacatttatt gaggttacaa ggtgctagta
    23881 agtattcaaa tataaatagc tctataaagt ctataattat agcagtaact attttagtaa
    23941 tcgtatgtac aattgctaca gtgtacaatt cctttagtat ttctataaat gaacgaaaaa
    24001 aacagtttgg gatattaaat tctataggtg ctacatctag tcaaattaaa agattagttt
    24061 ttatagaagg tattataatt agtttaattg gtataccaat aggactaata tctggaacag
    24121 tagctataga cctattgttt aaaattataa ataagtattt tactgaatcg gttgttacta
    24181 agatgagttt gcagatagtg tataatccaa taataattat tgttagtata ataatagttt
    24241 tgcttacaat ctttatatca attctactac ctgcaatatc ggcttctaat atttctcccc
    24301 ttaatgtgat taaaaatact ggagagtata aagtgggcaa aatcaaaagc tcaagactta
    24361 ttaaaatgat ttttaaaact gaaggagtac ttgcatataa aaatattagg agaaatagaa
    24421 aaaaatttat aataactctt ttttctttaa tggtaagtat aataatattt atatctttta
    24481 gtggctttac attactttta ctaagaggtg aagaaattag aaattctcaa agaaaatatg
    24541 atttgtattt aactgcaaag aacacagcta ggtctgtaga ggatactata agtgaactag
    24601 aagatattga tgggataaaa aactttgaat tggcaacagg acaatatgtg tctataagag
    24661 taagtgaaaa taaaataaat aaatccaagg aagatttaat caggaaatat tatcaaaaat
    24721 ataagattgg agatagttat gaatatgatt tcagtaataa tgaattaatt tttccaggag
    24781 attttgcagt aaaaaacaaa ataaataact tagtacaggg ctcttttaat aaagagagag
    24841 caattgaaga aaatggtgtt atattagtta gaaaaagtgc atttgaagaa aatgggaaga
    24901 aaggtgtagt agaacttacc aactataaag taggagatac agtaaattgt gaatatcttg
    24961 atgaaaatgc cttaagtaaa aaagtaaagg tcaagattct tgctataact gatgaagaac
    25021 gtctaggtct tggttatcaa aatatggggt tacaatttat aacttatgat gaagttgcta
    25081 aaaatttaaa tttaaaatta agtaaaagcc ttatatttat tgattcagga ggaagcatta
    25141 agactaagaa ggaaatagaa gctttggcaa ataaaaataa ttttaacttt tatgatgaaa
    25201 gtgcaagcaa tgaaagtgaa aaacaagatt taaaggttat taaaatattt gtatatggtt
    25261 ttatagtagt gatatcttta gtgagtgtta caaatattct aaatacagta agtacaagta
    25321 taaatctaag aaaaagagaa cttgcagtaa tacaatctat aggggttact cctaaaggat
    25381 ttaggaaaat gatatattta gaaagtttta tatatggtat actgtcattg ttgtttggaa
    25441 taccaattag tattggtata attctaatta tgaataaact tatttcaggt gtaattgaat
    25501 tttctccgat tataccgtgg acagccatag ttatctgtat agtaagtgtt tttataataa
    25561 cttttatagc agggtatata ccaatgagta aattaaataa agaaaatata
//
LOCUS       DAFKMX010000037        25076 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFKMX010000037 DAFKMX010000000
VERSION     DAFKMX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.037"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            9..1865
                     /locus_tag="LZB08_001254"
     CDS             9..1865
                     /locus_tag="LZB08_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBM7314765.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1948..2188
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2254..2489
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2574..3791
                     /locus_tag="LZB08_001255"
     CDS             2574..3791
                     /locus_tag="LZB08_001255"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBM7314766.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3908..4390
                     /gene="cdeM"
                     /locus_tag="LZB08_001256"
     CDS             3908..4390
                     /gene="cdeM"
                     /locus_tag="LZB08_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBM7314767.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4655..5920
                     /gene="hisD"
                     /locus_tag="LZB08_001257"
     CDS             4655..5920
                     /gene="hisD"
                     /locus_tag="LZB08_001257"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBM7314768.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6115..6867)
                     /locus_tag="LZB08_001258"
     CDS             complement(6115..6867)
                     /locus_tag="LZB08_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7314769.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFNY"
     gene            complement(6985..7254)
                     /locus_tag="LZB08_001259"
     CDS             complement(6985..7254)
                     /locus_tag="LZB08_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314770.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7729..8823
                     /locus_tag="LZB08_001260"
     CDS             7729..8823
                     /locus_tag="LZB08_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBM7314771.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8825..9775
                     /locus_tag="LZB08_001261"
     CDS             8825..9775
                     /locus_tag="LZB08_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBM7314772.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9768..10952
                     /locus_tag="LZB08_001262"
     CDS             9768..10952
                     /locus_tag="LZB08_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314773.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGMKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10965..12461
                     /locus_tag="LZB08_001263"
     CDS             10965..12461
                     /locus_tag="LZB08_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBM7314774.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12484..13434
                     /locus_tag="LZB08_001264"
     CDS             12484..13434
                     /locus_tag="LZB08_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314775.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13455..14417
                     /locus_tag="LZB08_001265"
     CDS             13455..14417
                     /locus_tag="LZB08_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBM7314776.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14789..15421
                     /locus_tag="LZB08_001266"
     CDS             14789..15421
                     /locus_tag="LZB08_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7314777.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15832..15924
                     /gene="kdpF"
                     /locus_tag="LZB08_001267"
     CDS             15832..15924
                     /gene="kdpF"
                     /locus_tag="LZB08_001267"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBM7314778.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15965..17647
                     /gene="kdpA"
                     /locus_tag="LZB08_001268"
     CDS             15965..17647
                     /gene="kdpA"
                     /locus_tag="LZB08_001268"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBM7314779.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17672..19729
                     /gene="kdpB"
                     /locus_tag="LZB08_001269"
     CDS             17672..19729
                     /gene="kdpB"
                     /locus_tag="LZB08_001269"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBM7314780.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19748..20374
                     /locus_tag="LZB08_001270"
     CDS             19748..20374
                     /locus_tag="LZB08_001270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBM7314781.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20611..21519
                     /gene="cysK"
                     /locus_tag="LZB08_001271"
     CDS             20611..21519
                     /gene="cysK"
                     /locus_tag="LZB08_001271"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBM7314782.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21546..22136
                     /gene="cysE"
                     /locus_tag="LZB08_001272"
     CDS             21546..22136
                     /gene="cysE"
                     /locus_tag="LZB08_001272"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBM7314783.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22296..22484
                     /locus_tag="LZB08_001273"
     CDS             22296..22484
                     /locus_tag="LZB08_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBM7314784.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22538..23407)
                     /locus_tag="LZB08_001274"
     CDS             complement(22538..23407)
                     /locus_tag="LZB08_001274"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBM7314785.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23604..24848)
                     /locus_tag="LZB08_001275"
     CDS             complement(23604..24848)
                     /locus_tag="LZB08_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314786.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9654 a         2673 c         4517 g         8232 t
ORIGIN      
        1 gttagaatat ggatacccat aataaatatg taaattttat aaaaaatata cctgtacctt
       61 ttttatattg tagaattgta aagagacaag aagatataga atatcgagta gaatatataa
      121 gtaagggtat gggcaaagta ttacaattag aagaaggtat ttgtgataaa aatatactag
      181 atgtattgcc tgtatttaag tctaaaaaat atttcaaaga gttgttctca aatgaagtgg
      241 attgtataaa aaggtacatt ccgacactta aaaactggat aaatataaaa aaacagataa
      301 taggggattc atacataatt ttgtattttg gtaaaattgt atttgattat agacagataa
      361 tcgattcctt tgataaaaag gaaaaagtag cttatattaa agacgaagaa ggcatatata
      421 tagattgcag tgaaaattta ataccaatat taaataacaa cattaaaaca acgaaagata
      481 tctttggtaa aaatgatata gaagtatggg gagagaatac aggaaaatta tttagagatg
      541 attatagaga aggagtatct agtaaaaaaa gatttttaca aaatcttttt gaatatgaag
      601 aaacattttt tatggttgaa aaatattttt tgtatgatga agacgaactt ttaggtacta
      661 tagggatagt agacaatata atatatagtg gttacagtaa tagaaattat aactcaaaag
      721 atttaatgaa gatgatagaa cactcaattc cagagaatat gttttataaa gatgtgtatg
      781 gaaattatat tggatttaat tcaggttttt tgaatttagc ttgtatgaat aaagaagaat
      841 tattagggaa gaatagttat aaaatatcag aagaagaagc tttaatagat aagatatttg
      901 aaagtgataa gggtgtagtt gaaaataaaa aagtagttac atttgagtta aatataagta
      961 tgaatgatga aaacaagtgc attgagatta caaaaaggcc gttttttgat agctatggaa
     1021 gtgttattgg aataattgga acagcaaggg atataagtag aagaaaaaga ttagaagagg
     1081 aaatggataa aactaggatg gaattttttg caaatttatc ccatgaactt agaacgccta
     1141 taaatttaat atcatcttca ttacaagtga tagaaaaaaa agaagcagat ttaatagaat
     1201 caaatgatac tttaaaaaga aatctaggta taataaaaca aaatggaaat agaatattga
     1261 ggttagtaaa taatgttata gactttacaa aaatgcaatc aggatattta gattttaaac
     1321 cagaagaatc tgatataatt gcatttatag aggagatatg tatgtctgta gctgattttg
     1381 caagccaaaa taatatacaa cttacatttg atacagaaat agaagaattt tcaatgttat
     1441 ttgattcaga aaaattggaa agaataatat taaatttact ttctaatgga attaagtaca
     1501 ataagaaaga tggaaaaata aatatatttt tatatgtgaa agataatgta tttaatatga
     1561 aaatatctga tagtggtata ggtataccaa aagaaaaaat agataaaata tttaataggt
     1621 ttgaacagat tgacaatgaa ctttcttaca gagttaaagg aagtggtatt ggactttcat
     1681 tagtgaaatc tttggttgag ttgcatgagg gaagtatatc tttaaaaagt caacttggta
     1741 ttggaagtga atttatagtt tcattgcctg ttagaagtaa aaataatatt gaaaaatata
     1801 atcataaaag agaaatcagc aatgaactaa gtaaaaaatt agaaatagag ttttcggatt
     1861 tataagttta aatataaaaa ataaaatttt aaaaaagtat tgacaattga tttagaaggg
     1921 tttaaaataa attacaacat aataaataaa aaggcgatga ataggaagag taaatctaat
     1981 tcgattacca gagagaagag tccgttggct ggaagacttt ttaaattaga gggattgaaa
     2041 actacctagg agctactttc tgaagtcatc tgcattagta gattttagta agaatagtcg
     2101 ttagctgcgt taaagtgtaa agtgagtttt catgacatat gaaaactaaa ttaggtggaa
     2161 ccgcgggaat aatactctcg tccttatgtt ataaggatga gagttttttg ttatttaaaa
     2221 atataaacag taatcataat aattatagaa aatataaaag caatgaatag gaagagtaaa
     2281 tttaattcga ctatcagaga ggaaagttca caggctgaaa gactttctaa gtttgagaaa
     2341 ttgaaaacta cctaggagct gtggtctgaa gttactttaa aaagttttag taagactaac
     2401 cgttaactgc gttaaagtgt aaagtggatt ttatcaataa atgataaaat caaattgggt
     2461 ggaaccgcga gtatagactc gtccctttat atagggatga gtctattttt attgcaataa
     2521 aagattgaaa ataaaaaaaa atcggaaaat tataaattta ggaggaaaat aaaatggaga
     2581 ataaagtgaa aattggagta ctaggatatg gagttgtagg aagtggactt atagatataa
     2641 tagataataa taaagaaaaa agaaatatag aaatagttgg aattttagtt aataatttgg
     2701 aaaaacataa ggacaaaaaa tattctaata taataactaa taatatagat gatatattta
     2761 ataaagatat agatatactt gttgaagtca tgggtggttt agaaccatca ttatcttata
     2821 taaaaaaagc attaaacaat aaaattcatg ttgttacagc aaataaagat ttgttagctg
     2881 aatgtggaga tgagcttgca aaattagcaa gtgaaaataa agtaagtatt aaatttgaag
     2941 cttcagttgc aggaggtata ccagtattaa aaccaataat agaatcacta gaaggaaata
     3001 atatagatag tataaatgca attttaaatg gaactactaa ttttatatta tctaaaatgt
     3061 atgatgagaa tctatcttat gatatggcat taagacaagc acaagaatta ggttttgctg
     3121 aagcaaatcc agagtcagat gtattagggt atgatgctgc tagaaaacta tcaatattat
     3181 caactctagc ttatgacaat agagtatatt ggaaagatgt gtatttagaa ggaattacag
     3241 atatagatga aaaagatatt gaatatgcaa aaaaattaaa ttgtaaaata aaattgattg
     3301 gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt tagacctgtt ttagtagaaa
     3361 aagataatat ccttgctaga atagacaatg agttcaatgc tgtcatagtg aatggagatt
     3421 cagtaggaga agtttctttt gtaggtaaag gagctggaag tttagcaaca ggaagtgcgg
     3481 tttattcaga tgttatagat ataatagata atagagtttc aagtatagat tcttttacta
     3541 aagataaaat acaagttaat aaaatagtta gagaaaagtg tggagcactt cttagattta
     3601 aaaaatgtaa taaagatgaa attctaaata tagttgagaa ttgtttagta aaattcgata
     3661 ttttaaatga tgatgatgaa cttgctatta tggtttatgc tgattctgaa tatgaaataa
     3721 acaacagttt atgtctaata aaagataaag gatactgcga aaaaatgaat aaaatgttga
     3781 aaataagtta aaaaagaaaa aatataagtt tatatgtaac atattttgac gattttaata
     3841 ctatatagta tgagatgaaa caaaacatct cataaaaaaa ttaaaattaa agggagagat
     3901 atgttatatg gaaaataaaa aatgttattc agaagattgg tatgaaagag gagaatctac
     3961 agctaaatgg ttccaaaatg atagagaaga atatgaaaga gaagcatatg atgaagatag
     4021 agaaagaaga ggttcaaact gtggatgttc agattcagga gaaaatagac ctagaaactg
     4081 tgaaagattt agaagagaag ctgagataag agaaagagaa gcaagagaag cattttgtga
     4141 atcttcagag aaaaagaaag aggcattagc atatgaatgt gaagctagaa aattatggga
     4201 agaagcagaa aaatactggg atgaatattc aaaatacaac tataaaggaa tcgaatattt
     4261 agcagaagct gctagattat ttgatgaagg tatggaatgt gaagctagaa gaaatggaaa
     4321 taatggagga aacaataata attgttgcca taaatgccat aaatgtaatt gtaactgctg
     4381 tagaaaataa aaaaattaat tgacaaatca atagttaaaa tatatcatat tatgtataat
     4441 aaaaagaaaa ttcagaaaaa aataatgatt agaagaaata taagtatatt tattagacaa
     4501 atagtgctca gttagtgagg tgagtacaat atgattaaac tcccatcctc tagcttttga
     4561 tagagggtgg gagtttttat ttattgatat atcttaaatg aggataatat tgtaaaataa
     4621 atgagtatga gattgtaaaa ttaggaggta aaaaatgaaa agtattaaaa catcaagtat
     4681 gaaagaaaag gtagatgaaa agatattaag agagcaagta agttctataa tagaagatat
     4741 aagaaataat aaggatgttg cacttaaaaa atataatgag aagtttgata gaaatacaag
     4801 agatgaattt agaattacta aggaagaaat aaaggaagct tataagcatg tagatgacga
     4861 atttataaat aacttaaaga ttgctgccaa aaatataaga gaatttgcta aagctcaaaa
     4921 actaagtttt gaaaaccctt ttgaaaaaga aatttatcca ggtgtaatct taggtcaaac
     4981 taatattcca atagagtcat gtcttgcata tgttccagga ggtcagtatc cattgttttc
     5041 aacagctctt atgcttataa tacctgcaaa agtagcagga gtaaaaagaa tagtggcatg
     5101 ttcaccaact atgaaaaata ctgaaaagat aaatcctaag acattggtag ctatggatat
     5161 agcaggtgct gatgaaatat atgcaactgg aggagttcaa gctatagcag catttacata
     5221 tggaacagaa aagattaatc cagttgacat aattgtagga ccaggaaata agtttgtaac
     5281 tgaagctaaa agacaatgtt atggacaagt agggattgat tttgtcgctg ggccaagtga
     5341 agttcttata atagcggatg aaacttcaaa tccagtttat attgcagctg atttattagc
     5401 acaatgtgag catgatttaa atgcaagagg aatattattg acaaatagct tggaagtagc
     5461 acaaaaagtt gaaaaaaata tagaaagtat gctaaaagat ttgccaacaa aggacatagc
     5521 atatagttca tgggaaaata atggagagat aatattggtt gatgatatgg aagaagccat
     5581 taaaataagt aatttttatg caccagaaca tttagaagtt gcagtaaatg agtataatga
     5641 tatttgtgat agattaacta actatggttc tttatttatt ggaaatcttt ctgctgaggt
     5701 gtttggtgac tatgtttcag gtaccaatca tacattacca acacttaaag catcaagata
     5761 tactggtggt gtttgggttg gaacttttat caaaacttgt acaaagcaga tatttaatga
     5821 aaaagctata caatcattag cacctgtagc tgaaaagctg gcaaaagaag agggattata
     5881 tgcacatgca aaggctgctg aggttaggtt taaaaagtag taaaaaattt tattttggca
     5941 gtagtattag actgagataa ggaaaacgct gagaacctga ataaatacta tattttgtgg
     6001 gtctcggcgt ttcttttata tctgtatata atagaataat actatctgtg tgtttaaata
     6061 ctaacttaat atttcttttc taaataatgt ttatgaaata gttaatattt ttagttaata
     6121 gttgaaaaaa acttttgcta actgtgcaaa ttcatctgca tttgtcaata tagcagggtg
     6181 tcctccctga ctaaaaatgt gaatacttgc ttttgaaata aaagttgcca tttgtttgta
     6241 ctcttcttca agattatgac gacacatttc gtcttcctta cttcctgtaa ataaaattgg
     6301 catttctaat ttacacaatt ctttatgaaa caaagggcgt ttctcgtttg cacattgcaa
     6361 tagagctttt gtatcaagat taacaacatt ctcccaatct tttccatgac accattcata
     6421 gaattttcgt gcttgaatat cttgctttga tttttccctt ccagtgatta gattatctat
     6481 gaagttatca ttaagagtac gtccatcaaa gctatctgct ataattgctt caactaaatc
     6541 gggacgttct aatccagtat taacagctgc ccatgctcct ccacttgttc caattaagcc
     6601 aactttacca caattcaaat gttcaatcag tgctattgtc tgtaaggctt catcatgcca
     6661 tatgtcagga gaaaattgag aaactctatc cgactgtcca ttacctaaaa aatcaattaa
     6721 gatacatcta aaattttcta catataatgg cacaagtgct ttaaacatat tagaagaacc
     6781 agtattacca tgcaggaaaa ttatcggttt accttgtcca tattctttat aaaaacaaga
     6841 attaccttga tactgaaaat atgacatata ttttcctcct ttttatttgt taagttaatt
     6901 ataacaatat aggtatgaat gaaaaacctc atgttctttc ttagaataaa aatgtgccac
     6961 gcataaaacg tgacacattt ctaattataa attttgctgt ttaagtattc tttgtatgct
     7021 tttctctgtc aaaaagtatt tctctgataa ctgcttgata ctcattccat tttggaaatt
     7081 tatacagatt tgagaattac gagaagctaa gacttgtttg gtgtttgtgt tttcacccca
     7141 atgctttttg tgctcttgct ttttgggaat ataaatcact ttaccatcta tatattcttg
     7201 cacttgctca atcagttctt taggcagtaa gtcaatcgaa ttttgatagc tcattttgca
     7261 cctcctaaat aattattctt taggattagc aaaggctact gccactcaca attaaaaata
     7321 aaatttttta ttatgtacaa tagcctttgc tatgcacaag ttccaattcc cataaactaa
     7381 atactccttt ctggtttgaa aaattcatct gaacattgat ttcgctttta agatagagtt
     7441 caatgacaaa tttacatttt tattatatat attcagatag ataattgcaa ctgtctttgt
     7501 atgaggatat tttattatag tcaatactat aatatgtatt tatatcatca aactaatatg
     7561 aacagttgaa aggaatatga acaataaaac ttagtcagga agaataatta tgaagcagac
     7621 aatagagaaa tttgatttta aagttttatt gaaaatttat tgacaaattt aacttgtaaa
     7681 tattatacta aacttaataa ataaagttta taaagtaagg tgttgtctat gaaaaatata
     7741 gttggaaaac cgcaaacgat aaaaaaagta aatgaagatt taataaaaaa cattataaaa
     7801 aatgaaggtc ctataacaaa accagaaatt tcaagcatta ctgaattgag tttagctaca
     7861 gttaataaga ttgtagagca attagctttg aaaaatgaag ttaaagtaag tggactaagt
     7921 gaatctactg gaggaagaag agctcaatta tttgaaataa atgcaaactt agaacacata
     7981 atagctttat attattatag aaattgttgt ataggggtag ttgccaattt attaggtgaa
     8041 ataatttatc aagaagaatt taatataaga atggatacat atgaaaacgt taaccaagat
     8101 actttttttg ttatagatac tctaataaaa aaggaaaaag gaaacaatat aagggctatt
     8161 ggaattggtg tcccaggagt tgtaaaaaaa ggtgtagtta aaaatatacc aactataaaa
     8221 agttgggaag gtataaattt agaaaattta ataaagagta aatacaatac taaagtgttt
     8281 atagaaaatg atacaaatat tactactatt gggatttatc aaaaacagta taaagacaaa
     8341 tataaaaata tggctcttat gtatttagaa gatggtattg gttcaggtat agttataaat
     8401 ggtgaattgt atttaggttc tacaaatttt gctggagagt taagttatct aaatatagaa
     8461 tttggtaagt ataactctaa caacttaaat cttgaagatt atattatgag tttaagaaaa
     8521 agatatttag aaagtaaaga taataaattt aagaaagaaa ttttatctat aatatcaaac
     8581 tttataagga ctcttgtatg tgtcctaaat ccacaagcta tgattataca gtgtagtata
     8641 ttaaccaaga gtgacatgga gtttatagaa aaagatgtaa gcaataatgt tggagaagaa
     8701 aattttccta aattaataaa agtagagtca ttgagagaag gaagtataaa tggagtaatt
     8761 agtatgtgtt taaaagaaac tgactatcaa tattccttat ctaataaaaa aggaggatat
     8821 taatgtgaaa tatttggttt caatagatgg agggggaaca aaaactaaat tttgtgtaag
     8881 tgacttagat ggaaacatac ttaaagaaca taccactggt tcaactaatt ataagtcagt
     8941 tggaattaaa aaaacatatg aaaatattaa taatggattt aaaaaaatat taaaagattt
     9001 atatatcgat tatgacgata ttgagtacac tgtatttggt atatctggtt gtgattctcc
     9061 taatgattat aagattataa tggatgaaat attaaaaatt gggattaaca aagaaaaaat
     9121 atatctggca aatgatgcag ttttagcttt ttatgcacaa gctgattccc ctggtttagt
     9181 aattgtggct gggacaggtt caattatact gggaattaaa gaagatggag aaatatatag
     9241 agttggtgga tgggggtata attttagtga tttgggttct ggatatgata tagggagaaa
     9301 attattaaag aaagtattgc tttattgtga tgaatgtcat gagtatagtg atttatttag
     9361 ttgtgtcttg gatttctttg gagcaaatag ttttgaacaa cttgcatata tgattactga
     9421 tataaatgat aatgttgaaa tagcaaactt agcttcatta gtgattgatt gtgccgaaca
     9481 aggtgataaa ttggctattg aaatattaag agaatcatca actgaactat ctaaattagc
     9541 tcaagtaata ttgagaaaaa tatctaatcc aatgaaaatg gataaaagcg aaataaatat
     9601 agttttgtct ggtggtacat taaataaaac tgtatatgct caaatgttaa tgaataattt
     9661 aaaaacagaa aatacagata aaaatttaaa atttatatct caagaaaatc agccagtata
     9721 tggggggata agacttgcta tggcattagc taataggagg gtcaagcatg gatagaaaaa
     9781 aggtaatagt acaaattttt actggaggtt ttaaggctaa agaaataaag tttaatgata
     9841 taaaatgtaa actactagaa ttattggact gtattgatat agaaaaggtt atcatgggat
     9901 ggagtgtaga tgaagaatta tatgtagaaa ctaaaaaact attaaataaa tataatgttg
     9961 aactatatct ttggttgcca tgtttttctg aaataggatt acttgaaaaa agtaatttac
    10021 taattgatta tatggggaag gaagtcaagg gctatgcttt acaagaggat gaaaattttg
    10081 agttttattg cccaaataat aaggaaaata taaaaaatat aaagaaaata tatagtactt
    10141 attttagtga tattaaattt gatggaattt ttttagataa aataaggtat ggttcttttt
    10201 cgaataaact aaatggtgtt tttaattgtt tttgtaaaga ttgtttaaat atatatgaaa
    10261 aaaataatat tgataaagag tcgttaatga gagaaatgaa taatgtgtct aagggagtta
    10321 tagataatga agatactcca tttggtataa cagaatacat taatggaaaa tacaaattta
    10381 aaaataaaat atgggaagat ttttttgaaa caaaaaatca aaatatatac aaggcattat
    10441 tagagataag taattatttt aaaagtaagg gattgaaaat aggtatagac actttttctc
    10501 cttttatttc ttactttaca ggtcaagatt tagaaaaatt acaagaagta gcagatttta
    10561 taaaaccaat gatgtataga actacaaaag ctccagcagg tttaccattt gaatttgaaa
    10621 ggtttattga tgaatccata cataagaata aagagattgg aaaaagaaat ttcttagata
    10681 ttttaggaat gaaggagtat gaaggagatg tatatcctat agaatttgta ctagaggaaa
    10741 tcgattttat gacaagaaat cttaataaag atatatatgt aggagttgaa ataaataaaa
    10801 aggaaattat agctcctgtt acacctaagt acataagtga aaatatttca aatataaata
    10861 agacaacagt aaaaggatat gttttatctt gggatttgtt gtcagcacca aaagaaaata
    10921 ttgatgaaat tataaaatgg tttcggaatt agtgaggtga taaagtgagt aagatattgg
    10981 aaatgcaaca aatatataaa agttttggaa aggtagatgt tttaaaaggg ataaattttg
    11041 agttagaaaa gggagaagta agagctcttt tgggagctaa tggagcagga aaatctacac
    11101 ttattaaaat tttaggagga gttcattccc aaactaaagg taaagtaatt ttgactggta
    11161 atgaaataat gtttaaaaat ccacatcatt ctaaagaaaa tggtattagt ataatttatc
    11221 aagaattaag cttgatacca acgcttacag tacttgaaaa tatttttctt ggtagagaaa
    11281 ttttaaaagg aagtgtgttt ttaaacaaga aagacatgct tgaggaatat atgaaaattt
    11341 gtgaggattt tgattttaat atagacccac atatagtagt ttctaattta agtgtagcaa
    11401 accaacaaat ggttgagata atgaaagctg tttcatgtaa ttctgaaatt atcataatgg
    11461 atgaacctac aacatcactt actaataatg aaaaagatgg gctatttaaa ataataagta
    11521 gactaaagtc taaaggtaaa acaatagtct atatttctca catattagag gaagttttta
    11581 aagtatgtga tagtgcaagt ataatgaaaa atggaattat gattggaact tatgatgtag
    11641 aggatttaac taaattaaag ataactcaac ttatgacagg aaatgataat cacaaactta
    11701 ttgagaagaa ggattataca tttgcaaaat atgaagagcc accagtacta gaagtaaaga
    11761 atataagctg gtctaacaaa ataaaaaatg tttcatttaa agtttataga ggcgaagttg
    11821 taggtcttgc aggtcttgta ggttcaagaa gaactgaaat tataaatttg atatttggta
    11881 tagataataa agatagtgga gacattttta tcaatggtaa aaaagtagac ataacttctc
    11941 ctaaggttgc tataaaaaac aaaataggat tgattcctga agatagaaaa aatctaggat
    12001 taattttaga acaagctata tacacaaact catcatcagt acaaattgat aaatttaaga
    12061 aattcttgtt tatagataga aataaagaaa ttgagttttc taaaaaaggt gttaaggaat
    12121 taggaataaa agttagtgat gtaaagcaag aagtaaaaga gcttagtggt ggaaatcaac
    12181 aaaaagtagt agtttctaag tggttagaga aagatttaga tttaataata tatgatgagc
    12241 cgacaaaggg aatagatatt gctgcaaaag aagatatttt caatacaatc aaagaatttt
    12301 caagtaaagg aataggagta atatttatat cttctgattt agaagaagta ataagagtaa
    12361 gtgatagaat tttagtaact agagaaggtt ctattgtaga tgatttaaaa aatgagaata
    12421 tacaggttca agatataatg aacagtatat ttaatgtata aaaaatgaaa atggaggact
    12481 gagatgaaga aagcttttga gaagttaaat ttaagagatt atggagtatt attaggtttt
    12541 ttagctttat gtatagctat aagtattgca actccatcat ttttaggtaa acaaaatata
    12601 cttaatcttt tacgacaatc ttctataatt ggaataattt cagcaggtat gacttttgtt
    12661 ataatatctg gaaactttga tatatcagtt ggagcagtag cagctcttgc aggtgctata
    12721 acaatgaaat ttgcgactgg tggaactaat ttgtttgcat ccatgctctt aggtgtagcg
    12781 atttgtgcag taataggatt aatcaatggt atagtagttg caaagataaa tgtaccatca
    12841 ttaatagcca ctatggctat ggtcactatt gtaagaggat tgctgttgat gcttacagga
    12901 ggatatccaa ttactgaaaa tataccaata cttgattaca ttggaaatgg atatttgttt
    12961 aatataccaa taccagtagt aattttcttt atagtggtgt tagtttcgtt tattgtactt
    13021 aataaaacta agtttggtag atatgtttat tctgtaggag ggaatcaaga agcaagtaaa
    13081 cttaatggta taaatgtaga ttctcacaaa gtgaaagtat ttattattaa tgctgtactt
    13141 gcaggaatag ctggaatcgt actaacagga agacttggga ctgcaactgc gattgcaggt
    13201 gaaggatatg atatggatgc aattgcatct gtagtaatag gaggtacatc agtggcagga
    13261 ggttctggtt ctgtacttaa aactgttata ggagtattac ttatgagtgt tataaacaac
    13321 agttttaatc ttcttggaat agatgtatat ttccagtaca tatttaaagg attaataatt
    13381 ttagcagcag ttggatttga ttcttatagc aagaaaaaac ttgcttcaag ataaaaaata
    13441 agggggattt ttttatgaaa aaattaatga aacatttagc attgttgcta tcacttgtta
    13501 tgatatttgg tttagttggt tgttcaaatg gtggtgacaa agaaaaaaat ggagaaaaga
    13561 aaatagcagt attactaccg ggttcaacag gttactttgt agcaactaaa caaggtatag
    13621 atgcaaaatc taaagaatta ggtgtaagcg tggaatatgc agatgctcaa tgggatgcta
    13681 gtaaacaatt atcacaagct gaagatttta tggctaaagg ggtagacatg attgcattat
    13741 gtggagttga ttctgctgta agtgagagaa tagttaaagc tgctaatgat tcagacgttc
    13801 ctatagtagc atttacaaat tctataggaa gtaacccaac tggagaattt aaaggtttag
    13861 ttacatatat aggacaaaat gaggaagaaa caggagcatt aactggaaaa attgctaaaa
    13921 acttattagg agaaactggt gggaaagctg tattaataga aggagtacca ggaactactc
    13981 cacaagtatt aagaaaaaaa ggtcttgaaa aagaattaaa agatagtaat atagaaatag
    14041 tttataatca aacttcaaat tgggagaaag agcaagcttt aaaagtaaca gaagatttaa
    14101 ttcaaaagaa aactgatttt aatgttataa tatgtcaaga tgataattct gctacaggtg
    14161 caggtcaagc tttaaaagat gctggtctta aggataaagt taaagtcata ggtttgggtg
    14221 gaagtaaaga tggattaaaa gctataactg atgggttaat agatggtaca acttatatgt
    14281 ctgctgtaga agaaggtgga ttagtcatag aaaaggcatc taaattcctt aaaggtgaaa
    14341 aaatagaacc tgttacacaa attaaacaag ttgaagttaa caaagataat atttcagagt
    14401 tcaagggtga atggtaaatt gtcatagata ataaataaga tttttagagt tactttagaa
    14461 actatataaa gtaactcttt ttttaattta atttagtgat aaattattga atagtatttc
    14521 taaacaaata aaaaattaat ataaacaaag acttaattat actaaattat aagtaaaact
    14581 aatataaact tatggaaaaa aggaaaacgt ctaaagtatt ggaattacta tagtgtaggt
    14641 gtggtattaa aaaaacataa aaaaacataa aaaaattgtt gaaaaaaata tagaaatttt
    14701 gtataattaa agatttgaaa agtgcggtat acaaagccaa ttcttttgta tataatatat
    14761 ataaattcag aaaaaggaag ggatatgtat ggccaggcgg ttatttttag aaaggattgg
    14821 acttatattt ttaggttcag caatattagc atttggagtt tataattttt attatttaaa
    14881 caacataaca gaaggtggag tgttaggtat tttactactg ttaaaaaatt tattcaatat
    14941 acaaccagca atagcaaatg ttgtaataga tggtttacta cttttagtag gatataaatt
    15001 tttcggtaaa aaattcctta tatattcaat agtagcttct ataacttttt cagtgttata
    15061 tgacttattt gaagctatag gacctctagt accacaatca caaaatatgc ttttatcaac
    15121 tattttggca ggtgtaactg ttggaactgg tgtaggtata gtagtaaaag caggttgtgc
    15181 atcaggagga gacgatgctt tagcattagt aatttctaag actacatctt tgaacatagg
    15241 gcaagtttat ttagcaacag atgttattgt attgttgtta tcattatttt atctatcagc
    15301 gtttgatatc ttttattctt tgattgctgt tacaattagt ggcaaggtaa ttgattttat
    15361 ttattatcat ggtaaaagtt tggatatgga tataagtaat gatatagttc cagaatgtta
    15421 atataaatta aataacttta gattttatta ggggtggcag atttgccacc ttttttgtgt
    15481 ctaaatcttt atagaaagta taatcccatc taggtttttg taaatctcaa atatagaatt
    15541 atgttggaat gactgggaat acgattgcta gatgtgcatt ttaaaaacca aattttatat
    15601 ttatgaaata ctaatcatta atggatgttt cttaatccta tctttatacc taaaatgata
    15661 agataaatga agtagcaata caaaactaaa atttgtaaaa gagattaaat taaatggagg
    15721 agctaaaatg ttggatatag ttatgatagc attgttattc ataggatttg tttccatgaa
    15781 gttacttgct gattggtgtg ggaaacaaac tgaaaaaaaa taatgtaatg tatggaggga
    15841 ttacctatgt ggtttctggc agttgtaatt ataatgttaa taatatattt ggtatatgcg
    15901 cttattaatc ctgaaaaatt ttaaacagta taaggctaat aagtaagttg aattgggggg
    15961 aacaattttt atggtgcaga tagtaatagt gttagcgatt tttatgattt tagtaatacc
    16021 tatgggcaag tatttatatc atatagctac taatcaaaaa acatttggag atagactatt
    16081 tgataaagtg gacaatttta tatataaggt atgtagtata gataagaaaa aagaaatgaa
    16141 ctggaaacag tatgcattag ctttattatt tacaaatgca gttatggtat ttataggata
    16201 tatcatactt agaactcaaa gtatgcatat ttttaatcca agtggaatta aaagtatgga
    16261 acaaggacta tcatttaata caataataag ttttatgaca aatacaaatc ttcaacatta
    16321 ttcaggtgaa tcaggacttt catactttag tcagatgaca gtgataatat atatgatgtt
    16381 tacatcagct gcaacaggat atgcggctgc tatggcattt gtaagaggtc tagtcggtaa
    16441 aaagaaaaca ttgggaaatt tttatgtaga tttaattagg ataacaacaa gagtactttt
    16501 accaggagct ttaattattg ggcttatatt agttacacaa ggagtaccac aaacttttgc
    16561 tgggacagaa acagtgacta ctatagaagg aaagcttcaa gatatagcta gaggtcctgt
    16621 ggcagctctt gaatcaataa aacatttagg aacaaatggt ggtggattct ttggttcaaa
    16681 ttcatcacac ccatttgaaa atccaacaat aatatcaaat atagtagaaa tattatctat
    16741 gatgatatta ccaggagctt gtgttgttgc atttggtcac atgataaaga ataaaaaaca
    16801 aggttgggtt gtatttggcg caatgtcaat catattttta ataggtctag tagtgtgctt
    16861 taaagcagaa agtgcaggaa atcctatttt atcacaatta gggcttaatc aaagtatggg
    16921 aagtatggaa ggtaaggaag ttagatttgg tatagcacaa tcatcattat tcacaacagt
    16981 gactacttcc tttacgacag gtacagttaa taatatgcat gatacactga ctcctttagg
    17041 aggattagta ccacttttaa atatgatgct aaatgtagta tttggtggta aaggcgtagg
    17101 tctgatgaat atgttgatgt acgctataat agcagtattc ttatgtggat taatggtagg
    17161 aagaacacca gagtttttaa ctaagaaaat cgaaggaaaa gaaatgaagc ttattgcttt
    17221 acttattata ttacatccac ttttaatact aatgttctct ggtctttcag tagcaatacc
    17281 agcagggctt gaaggaattt caaatcctgg attccatgga ttatcacagg tattgtatga
    17341 gtttgcatct tcagcagcca ataatggttc aggatttgaa ggtctaggag ataacactat
    17401 gttttggaat ataacaacag gtatagttat gttctttgga agatatgtgt ctattatagt
    17461 actactagca atatcaagtt tgttagcatc taaaaaagct gtaaatgaaa gtataggaac
    17521 acttagaacg gataatttta catttacaat agttttagta cttgttgttt taattgttgg
    17581 tgctttaaca tttttcccag ccttagcgct tggacctatt tcggagcatc tagtgttgtg
    17641 gcactaaaaa attgggaggg agtaaagttt tatggaaact aaaaaaacaa agtttattac
    17701 aaaagatatt ttcaaaactt ccattataga agcttttaaa aaattaaatc ctaaatatat
    17761 gatgaaaaat ccagttatgt ttgttgtaga aattggattt atagtaacat tgatacttac
    17821 aatagtacct agtttgtttg gtgatgaagg caataactta agagcttata atgggattgt
    17881 aacaataatc ttatttataa cagtcctttt tgcaaatttt gctgaagctg tagctgaagg
    17941 aagaggtaaa gctcaagcag atacacttaa gaaaactaga aaagatacag tagcaagggt
    18001 tatagatacg ttaggaaatg aaaaaatgat aagtgcaagt gagctaaaac ttggtgatat
    18061 tgttttagta aatgctggag aggttatccc aaatgatgga gaagttatag agggtatagc
    18121 ttctgtggat gagtcagcaa taactggaga atctgcacct gtcatgagag aatcaggtgg
    18181 agatttcgca tctgttacag gtggtactac agttgtaagt gactggttaa agataagaat
    18241 aacaacaaaa ccaggagaat cgttcttaga taaaatgata gctcttgtag aaggtgcttc
    18301 aagacaaaaa acgccaaatg agattgcttt aaatactttg ttagtagggt taacacttat
    18361 atttatggtt gttttaatat ctctttatcc aatggcaaac tatgcaggtg taaagattcc
    18421 aatttctact atgatagctc ttttggtatg tttaatacca actactatag gtggattgtt
    18481 gtcagctatt ggtattgcag gtatggatag agtaactaga tttaatgtaa ttgcaatgtc
    18541 aggaaaagca gtagaagctt gtggagacgt tgatactatg attttggata agacaggtac
    18601 gattacttat gggaatagac ttgcatcaga atttatagca gttggaaatg cagattcaaa
    18661 agatttaata aattattctg ttatgtgttc attaaaagat gatacaccag aaggaaaatc
    18721 tatagttgat ttaggtatga aattaggttc aacaggtaag acaaaagagg cagaggaagc
    18781 tgagtttgta gaattttctg cacaaactag aatgagtggt attaacttag ctgatggtag
    18841 agctataaga aaaggtgctt atgatagcat aataaaaaga gtaaaagaat ctggtggtac
    18901 agtgccagaa gatttagaag aaaatgtaaa tcgagtagct aaactaggag gaacaccttt
    18961 agtagtgtgt gtagataatg agatatatgg aattatctac ttaaaagata cagtaaaatc
    19021 agggcttgta gaaagatttg ctagacttcg agagatagga ataaagacaa taatgtgtac
    19081 aggggataat cccttgacag cagctacaat tgcaagagaa gctggagtag atggatttat
    19141 tgcagaatgt aaaccagaag ataaaataga tgcaataaaa aaagaacaat tagagggtaa
    19201 aatagttgca atgacaggtg atggaacaaa tgatgctcct gcacttgctc aggcagatgt
    19261 aggtcttgca atgaatagtg gtacaacatc tgcaaaagaa gcagcaaata tggtggattt
    19321 agattcagac ccaactaaga tattggaagt tgttgaaata ggaaaacagc ttttaataac
    19381 tcgtggggct ttgacaacat ttagtatagc aaatgatgtt gcaaaatatt ttgcgattat
    19441 accagctata tttactcttg caatacctca aatggagata ttaaatgtaa tgaaactttc
    19501 tacgtcattt agtgcgatat tatcagcttt aatatttaat gctataatta tcccatgttt
    19561 aatacctctt gcaatgaagg gagtaaaata tagacctatg aaatcacaag ctatgttaat
    19621 gagaaatatg cttatatttg gtcttggtgg agttatagta ccatttttag gaataaaatt
    19681 aattgattta ttgattacac ctttagtaat ggttcttaat ttaggttaat aaatggagga
    19741 tataaaaatg aaaacattaa agccagcatt acttgtaagt attgtattgt tagttgtttg
    19801 tggacttgta tatccacttg ttttgacagg tgtaagccaa gttgcattta aagataaagc
    19861 caatggaagt atgatagaag taaatggtgt aaaagtaggt tcagaactta ttggtcaaag
    19921 ctttacagat gcgagattct ttaagggaag agtatcatca gtaaactata atacttatac
    19981 aaaagaagat ttagtaccag ataaagatgg aaatgcatct tatggaggtg tatcatcagg
    20041 gtcttttaat tatggagcta caaatccaga acttcatgat agagtacaaa aagatataga
    20101 aaagttttta aaggataatc caactgtcaa aagagaggat ataccaacag atttacttac
    20161 agcatcaggt tcaggacttg accctaatat aagtccagcc tctgcaaaaa ttcagatacc
    20221 agctatagca aaagcgtctg gaatatcaga aagtaaatta caaaaaattg tagatgataa
    20281 tacttccaaa aaactttttg gagttttagg tgaagataga gttaatgtac taaaggttaa
    20341 tgtagaggtc gcaaaaatat taggtcttat atagtatttt gtagaatttt agtaacaaaa
    20401 tattactcaa gatttaattt aaaggttaaa ttttaaagag tgaaaatcaa taatgatttt
    20461 cactttttta tttgaatatt actaatattt ggaataaagt gattgtaagt ataaaatcca
    20521 aaaaaaggtt gcattatcaa aatgataata ctataatata tttatcgtta tcaatttgat
    20581 aatagatttg gacatatagg aggaaatagt atgttatata ataacgcatt agagttgata
    20641 ggaaacacac ctgtagtaag gctgaataat ttaggctgta ctaatgggtg ttctaacata
    20701 tatgtaaaat tagaaaaagt aaatccagca ggaagtataa aagatagagc tgtttatgaa
    20761 atgttagagg gtttagagca aagaggagaa ttaaaaaagg gagatgtact agttgaagca
    20821 actagtggaa atactggtat tgcattatct atgataggaa aattaaaagg atatgaagta
    20881 gtaatcgtaa tgcccgaaac aatgagcgtt gagagaagag atttaatgaa ggcttatgga
    20941 gcaaatttgg tgcttacaga tggtaaagct ggaatggcag gttccataga aaaagcaaat
    21001 gaacttttaa aagaaaatcc aaactataaa agcttaaaac aatttgaaaa taaagataat
    21061 ccaaatgcac attataaaac aacagcagtt gaaattatga atgatgtcaa agatttagat
    21121 atattcgtat gtggagttgg aacaggtggt actttaatag gaactgcaag atatttaaaa
    21181 gaacaaaatc caaatataag agtaattgca cttgaaccaa aaaaatctcc agctatatca
    21241 gaaaacaaga ctggacctca taaaatacaa ggaataggtg ctaactttgt tcctgaaaat
    21301 tatgattcta gtgtagttga tgaagtaata ttagtagatg atgaagatgc atttgataca
    21361 gttaaattac ttgcagcaaa agagggaatt ttagtaggga tatcttctgg agcaaatata
    21421 tttggtgcta tagagatggc aaaaaagtat ccagataaaa agatagttac agtagctcca
    21481 gatggagttg ataagtatat gtctatggga atattttaat aattttattt gagggagtga
    21541 atactttgtt taagaaaata aataaagata ttgaatatat aatgaagaat gatccagcag
    21601 ctaggtctaa gatagaagtt tttttacttt atccatcagt tcatgcaatg ataatgcata
    21661 ggatggctca tgctctttat aagaagaaga agctttttac agcaagatta atatcccaga
    21721 tatccagatt tatgactggt atagaaatac atcctggtgc taaaatgggg gaaggtatat
    21781 tgatagacca tggaatgggt gttgtaattg gagagacagc tgaagtaggt aatagggtga
    21841 caatatatca aggagctact ttaggagcta caggaaaaga tactggaaag agacatccaa
    21901 ctgttggtga tgatgtatta attggtgctg gaacaaaaat actaggacct ttaaatatag
    21961 gttcaaattc aaaaattggt gcaaattctg ttgtcgttaa ggatgtacct aatggtgcaa
    22021 cagttgttgg tataccagca aaaatagtaa aaataagaaa cctagaacct gtaaaaaaga
    22081 ataaaaaaga agtgtcctat gagtatgatg aattagataa tgtatattat atataggatt
    22141 tatataacta taaaataaac tttataaaaa ttattttaaa aggtattttt ttcaaatttg
    22201 aggacataat aaaaaagaac tgaatgttac agttttggtt catcaatctt tgataaacaa
    22261 gattttaaat ttgtagcgtc taggaggatt ttattatgaa agcaaatgtt aatcaagata
    22321 cttgtatagg ttgtggatta tgtccatcaa tatgtccaga agtatttgat atgaaggatg
    22381 atgggaaatc acatgttata gttgatgaag taccaagtga tgctgaagaa tcagcagcag
    22441 aagctagaga aagctgtcca gttgatgcaa tagatgtgca ttaataaaat aaatgcctat
    22501 aaaaattgaa ttttcaattt ttataggcat ttgtctttta ttcagttagt atttcaacac
    22561 ctgtttctgt aactaaaacc atacattccc attgagcaga taatttacca tcttttgtat
    22621 aaatagtcca attattttct tcatcaacaa aaatttcgtg agttccttca tttaccatag
    22681 gttctattgt gaatatcatc ccaggaacca atagcatctc agtccctttt ttagatacat
    22741 aacttacaaa agggtcttca tgaaagctta atccaactcc atgaccacct atttctctaa
    22801 caacagagta tccatttttc tttgcataag agttgatagc atcacctata tctcctaaaa
    22861 atccccatgg tttggcaact tcaagacctt tttggacaca ttcctttgta acttgaacta
    22921 atttttttgc attttcatta acattaccta tcataaacat tcttgaagca tctgaatagt
    22981 atccattaaa aatagtagaa acatctacat ttataatatc tccatctttt aagataatgt
    23041 ccttacttgg aataccatgg caaacttcat tatttactga tgtacataca cttttaggaa
    23101 atccattata gtctagtgga gcagggatag ctccattttg tagtgtaaag tcatgaacca
    23161 acttatttat atcttctgta gacattccaa tttcgatttt atctgaaata taatccaaaa
    23221 tttttgtgtt tattacagca ctttttttta tttcctcaat ttgactttca ttttttataa
    23281 tatcatgagt tggtacaata tgacctaact tagcaaatga atctatttta ttatccatat
    23341 ccatatgaca ttttttatat tttttagtac ttccacacca acagctatca tttctattta
    23401 acttcataac ttttcatcct tcttttatta tttatttaaa ttatgggtta cataaactat
    23461 tttaaaacat aaccattata aatattataa cataaatact aagaaatcct ttatagatag
    23521 tgatttattc aaagtataaa ttattatatg gttattgttg atgaaaagag gatagttaaa
    23581 tgtattattg gatattagcg cacttaatag tttagttcaa gtttttcaag aaaatcatat
    23641 gttagaggac aattttcctt taaagtattt tttgaatctt catcaagata ataatgtaca
    23701 aatgattctg caaaaaattc ttctataaac ttgcttatat atgcatgtgc aacttgctta
    23761 gggtcataaa gcttatatct ttcctgagca aatacttcca taaattcttc agaatttgat
    23821 tttttattta aaacattttt atctatagca tgtgcagttt catgtaattc taaatttttt
    23881 gatgaatgta catttgcata gctaggatta ctataaccta ttttagccac tatagaatta
    23941 tttctatata taccaggtac atctttccaa gtcttgcctg atcttaccca acatggaggt
    24001 aattgatatt ttaaatcact aaattctggt tcatcagtta gatttgaatt tattaatttt
    24061 atttttatat ccttttcttt aagtgtctgt aaatatttaa tatttatttt tgcaagtctg
    24121 tttttcatat tttcaacttc atctttatca taattcaagt catcaggaag tataagcatt
    24181 tgttcaatta tatctatact actgtctttt atattttcag attcttcatc aaatttatat
    24241 tgaagagtat tattttcata gatagttata tttgtattat tttctataga ataagagtga
    24301 atatcctcta aatctaaaat attcatttgt tgtaaaatat gttttgtttc gccactccac
    24361 actactaaag aaagtgaatt aaaattaatt agtggatttt cttttgcttt tatatcattg
    24421 cttatatcta catcaaatac atattcaatt gagtcttcta tgttaagact atctgtattc
    24481 tttttaatat ataaatacgc ttgttttaag tctatataaa tctcatctaa agattcgtca
    24541 taaaattctg ataaattatt agtaaagagt tcttcactta aaagttcatt tttatcagtt
    24601 atactttcaa ttaaaatatt aatttctttt atcatatcgt aaatacttat ttttctattt
    24661 gctttttcac tagatacatt gcatggcgca agagcaatgc aataaaaatc tttttctttt
    24721 ttataaaagg ctttaagtga ttttgataaa agtgtaaagt ctacacctat ctgtaagttg
    24781 accttaagtg agtcattttg taataattca gtattatttc ttttagctgt atttaaattg
    24841 ttttccataa tcattgcccc ctttttatta gggtatcata atttttttat atgtgtcaat
    24901 ttattttttt gaaaattata atttttttga aaattttatt ttttataatt tgtgaatttt
    24961 ttattataga aataaatatg ttattattat gtcaaaattt agcattagat tcatgtagag
    25021 tatgttttat acatatatta taattaattc tttgaagatt gcatattcaa aataca
//
LOCUS       DAFKMX010000038        24972 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFKMX010000038 DAFKMX010000000
VERSION     DAFKMX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.038"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..746)
                     /locus_tag="LZB08_001276"
     CDS             complement(<1..746)
                     /locus_tag="LZB08_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7314787.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCS"
     gene            963..2129
                     /locus_tag="LZB08_001277"
     CDS             963..2129
                     /locus_tag="LZB08_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBM7314788.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            2151..3797
                     /locus_tag="LZB08_001278"
     CDS             2151..3797
                     /locus_tag="LZB08_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBM7314789.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            3894..5267
                     /locus_tag="LZB08_001279"
     CDS             3894..5267
                     /locus_tag="LZB08_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7314790.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWAKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQDYIG
                     VIFSSAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5712..6665
                     /locus_tag="LZB08_001280"
     CDS             5712..6665
                     /locus_tag="LZB08_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBM7314791.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6703..7665
                     /locus_tag="LZB08_001281"
     CDS             6703..7665
                     /locus_tag="LZB08_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBM7314792.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7690..8676
                     /locus_tag="LZB08_001282"
     CDS             7690..8676
                     /locus_tag="LZB08_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBM7314793.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8689..9735
                     /locus_tag="LZB08_001283"
     CDS             8689..9735
                     /locus_tag="LZB08_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBM7314794.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            9902..11632
                     /gene="lpdA"
                     /locus_tag="LZB08_001284"
     CDS             9902..11632
                     /gene="lpdA"
                     /locus_tag="LZB08_001284"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBM7314795.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12050..14998
                     /locus_tag="LZB08_001285"
                     /pseudo
     CDS             12050..14998
                     /locus_tag="LZB08_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            14976..15440
                     /locus_tag="LZB08_001286"
     CDS             14976..15440
                     /locus_tag="LZB08_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7314796.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15553..15855
                     /locus_tag="LZB08_001287"
     CDS             15553..15855
                     /locus_tag="LZB08_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7314797.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            15917..17281
                     /locus_tag="LZB08_001288"
     CDS             15917..17281
                     /locus_tag="LZB08_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBM7314798.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            17316..17789
                     /locus_tag="LZB08_001289"
     CDS             17316..17789
                     /locus_tag="LZB08_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314799.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            17961..18611
                     /locus_tag="LZB08_001290"
     CDS             17961..18611
                     /locus_tag="LZB08_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBM7314800.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18785..19717
                     /locus_tag="LZB08_001291"
     CDS             18785..19717
                     /locus_tag="LZB08_001291"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBM7314801.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYYDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKNRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            19991..20704
                     /gene="ispD"
                     /locus_tag="LZB08_001292"
     CDS             19991..20704
                     /gene="ispD"
                     /locus_tag="LZB08_001292"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBM7314802.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20701..21186
                     /locus_tag="LZB08_001293"
     CDS             20701..21186
                     /locus_tag="LZB08_001293"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBM7314803.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21281..21489
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21553..23268
                     /locus_tag="LZB08_001294"
     CDS             21553..23268
                     /locus_tag="LZB08_001294"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBM7314804.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23390..24835
                     /locus_tag="LZB08_001295"
     CDS             23390..24835
                     /locus_tag="LZB08_001295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBM7314805.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
BASE COUNT        10038 a         2658 c         4712 g         7564 t
ORIGIN      
        1 atactgcata aggtatcatt tgtgttaatt aaatctttat aaatatgaga aatacgcaca
       61 tcatttaaat actctaagta agtttgtccc atatactgtt taaattttct acaaaaatat
      121 tctggttgta agtgtgctac cttagcgatt ttgttaatag aaataggcat tttgtaatta
      181 atctttgtat attctaaaac tggttcaaat tttgacaaat caagtactgt ttttttcata
      241 tttttattac ctacagatat tttaaagttt gtatataatt caaataaaag ttcaaataat
      301 aagcttgtaa agagcagatt agctgcagga ggagaactaa gtttaatttc tctcatgttt
      361 tctaatataa gtttaacttt acttgaattt tttttatagt tattgtcatt ggcattaacc
      421 ttaaagtcaa aatagcaatt tttaaaatca gggatatatt tgtttagaaa agacaatggt
      481 acttgcaata atatagaggt attttgataa atacatctag ttgagtgaat aattccagaa
      541 ttaatgaaca tacactcatt agcttttaaa ttataagtgt aaccctccat gttaatttgt
      601 aaatcaccag aagttatata aattatttct aatgagttat gccaatgatt tgctacataa
      661 ctaccactat catcaaatag tttgaaatta acatctaaat tatcaatagg ttcaattatt
      721 tcatgtgaaa aattatttct gttcataata atatcctcct tataatttta aattataaat
      781 agtaaattta taatgttcaa tattaaaaag ataatataga ctaataaatt agataataaa
      841 catacttaag aaaacggata aaatcaaata taataactgt atattaaaat aacttaaaaa
      901 aattatttta ttataagtgc aaaatatacc tatttaaata agataagagg ggaggatttt
      961 atgtgtatat aaaaggtgta aatttaggag gttggttagt tttagaaaaa tggatgacct
     1021 caagtttatt tgaaggcaca gaagctgaag atgaatacta tttaccaaga cagctttcaa
     1081 gagaggcata tgaatctaga attaagactc atcgtagtga atacatcaca gagagagatt
     1141 ttgcaacgat taaatctatg ggatttaatt cagttcgtat accagtacca tactttattt
     1201 ttggagattg tgaaccattc ataggatgtg ttaaagaact tgataaagca tttgcttggg
     1261 cagataagta tgggcttagt atattgatag acttacatac agttccagga agccaaaatg
     1321 gatttgataa tggaggaata tcaggtatat gtagctggtc acaaaatcca gaatatgtag
     1381 cgtttacatt aaatgtatta gaaagacttg caaaacgtta tgggatgaga catgaattgt
     1441 atggaatcca aattttaaat gagcctataa cagaaagaat gtggaatata atgaatgttc
     1501 caaatcggtt taaggctgta gataaggaga tggcaagggg gagtaaacct aattcattag
     1561 agtttttacg tgatttttat ataaaagcat ataaggtaat gcgtccttat atgagagagg
     1621 aaaacgttat agtgtttcat gatgcatttg aattaaaagc atggaaagac tttatgagag
     1681 aggaagaatt taaaaatgtt gttctagata cacaccaata tttgatgtta gctgaagcag
     1741 atggatgtga acaaagtatt gatagctatc tcaaatatat tagagaaaat tatgctaaag
     1801 atattttaca aatgcaaaaa tattttcctg ttatatgtgg ggagtggagc ttatttaatt
     1861 catatgcttg tggtatagat acaaatggag gtcaatcacc tctaaatggg attgaatcaa
     1921 atatagataa attatcaaaa gacgataaaa gagaattata taggaagata gcaaaagcac
     1981 agcttgatgc atggagaaat ggaagtggtc actactattg gaattataag ttattactag
     2041 atacagttaa tgaagaaggt tggataggat gggatagttg ggacctaggc aaatgtgtag
     2101 ctcaagaatg gtaccctata gaatattaaa ctaaaattgg aggaaaagat atgattaaaa
     2161 accctatact tccaggtttt aatccagatc cttgtatttg tagaaaggga gatgattatt
     2221 atcttgtagt ttcatctttt gaatggtttc caggtatacc tgtttatcac tctaaagact
     2281 taaaaaattg ggagttatat actcatattt tgacagatga aacaaaaata gatttgaaga
     2341 agcttccttc atctaaagga atttgggcac cctgtttgac ttattgtgaa gaagaagatt
     2401 tattttatat agtttatgga attatgaact ctatgaatgc aagatatttt gatgttgata
     2461 attatttaat tacatcaaaa gatataaagg gagagtggag tgaaccagta tatctacact
     2521 catcaggttt tgatgcgtct atttttcatg atgatgatgg taaaaagtgg attgcatcat
     2581 tagactggga gactagagaa ggatatgaaa aacctggtgt tatatgttta gtggaatact
     2641 gtacaaaaaa gaaagaaata gtaggatatc caaagagaat atggtcaggg ggaactgata
     2701 gaggttgtat tgaagcacca catataacaa agcgtggaga ttactattat ataatgtgtg
     2761 cagaaggtgg aactggatat ggtcatagtg taactatggg aagggctaaa aatatatggg
     2821 gaccttatga aaaagattca atgaatccaa ttgtaacttc aattccaggc gatttttatg
     2881 aaagacatga ccctgaccat ctaaagccta aatactataa tccagaatca aaacttcaaa
     2941 aatctggtca tggaagctat atagaaacaa catcaggtga agtatattta gttcatctaa
     3001 catctagacc atttgtacca gagttaaggt gtactttagg tagagaaaca gctattcaaa
     3061 aaatgaaatg gacaaaggat aattggcttc gaatggaaga tgaaagtaat ttggcaaaag
     3121 aatacgtatc agaaagtaaa ctagaagaac atttagtttc atctatacca agttttgatg
     3181 actttgattc aaatgaatta ggtttacagt actatgctcc taggatttca ccattatcat
     3241 ttgcagatgt taagtctaga ccaggatatg taaggattag aggacaagaa tcaagaacat
     3301 cattaaataa agtgagcata ttggcaagaa aattaacttc agtatatgca agaataacca
     3361 ctaagatgga gttttaccca gaggtacatc agcatagtgc gggtttgata atgtattatg
     3421 ataatatgaa ttatataaat ttaagaaagt attatagtga aacattgggt caaagtgcac
     3481 tatcaataat acatcttgaa aatggagaga agacggaatt tttaaatact aggataccaa
     3541 taaaagatat accaatatat cttcgtctgt atatacaagg tcgtaagtca tattttgaat
     3601 ggagttatga tgaaaaaaac taccagagaa taggaaaggt atttgataca actaagtttt
     3661 cagatgagta ctgtaaatat ggagaattta caggtacgtt tatagggtta acttgtgcag
     3721 atcgtgtaaa gcataaacac tatgctgatt ttgatttttt tgaatatata gtagatgaat
     3781 caaaagatgt cgattaataa aatcattact aaaattttca gagcaaaggt aaatcttatg
     3841 ctctgaaata agaaacatta gaaggcaaat cttacaaaaa ggagtatggt attatgaata
     3901 cagaagttaa tgttaaagca actactatta gatatgaaaa gatttcattt tttgaaagaa
     3961 tttcttatgg ttgtggggat ttggggtgca atatcatata ctcagctatg tcagcatttt
     4021 tgctgtttta ttatactaat tatgcagatg ttagtgcagc agctgtaggt agtattatgc
     4081 ttgtatctag gatactggat gggtttagtg acttaacaat gggaattata gtggatagaa
     4141 ctaaatccaa atacggtaag gctaggcctt ggattttgag aatggcgatt ccttttgcta
     4201 tagcagcagt attattattt tctgttccat caaatcttgg tatcacttct aaattaattt
     4261 atatttttat tacatataac ttagtttcta ctgttattta tacagcaatc aatgtaccct
     4321 atgcaactct aaattcactt ataactcaaa accagtatga aagaggagta ttaagtatat
     4381 ttagaatgat attagccact tgtggtacct tactaataaa tggacttaca ctacctctag
     4441 ttgaatactt tggaaataat ttatcagcat gggctaaaac attctttgta tttggaatag
     4501 catctataat ggtatttttt ataacattta ctggaaccaa ggaaagagta aaagcagtta
     4561 agcaagatag gaatgaagta ataccattta aaataggtat taaatcttta tttagaaata
     4621 agtactggat acaaataaca ttatgtttag tttgcatatt tattgtattt gcgataaatg
     4681 gtggttcatc cgtatattat gcaaagttta ttttgggaga tgaaaaatta tttggtccta
     4741 taaatatggt ttcaaatata tcccaaatta tagctatgtt tatggttgct ccttttataa
     4801 aaaaatttgg aaaaagaaat gtattaatag taggttcaat aatattaata tcgtcaaatg
     4861 ttatgtttat aatagttgga caagactata taggagtaat attttcaagt gctataaagg
     4921 gaataggaag tgctggaata gcatcaacaa tgtttgctat tgtttcagat actattgaat
     4981 atggagaatg gaagactgga tatagaactg aggggcttat aaatagtgca tctagttttg
     5041 ggtttaaagt gggaaatgga ttaggttcag caatattagg tgcagtatta tcaattggtg
     5101 gttatgtagg aacatcagct actcaaagtg atttagctat agtatcaata aaagtatgtt
     5161 ttatatacct acctatattt ataacaatat tacaaattat cataatgtct ttttataagc
     5221 ttgataaaga atatagtact atacttaatg aattaaatac taaataatga aaaagtttct
     5281 tataaagtct taagtttaat taaggtctat tgtaaatgtt ccctataaaa actattatat
     5341 atttatgatt attgaagaat ttttagagaa gcgaataata aaaatataag aagactttaa
     5401 atgatgaaat ggttggtttt ttagaaatga tttaataagt tattgttatt taaagcatta
     5461 taagtggaca attaattttt aatttatgga gatgatattt aacaattaga agtaatatat
     5521 aatatgcact aagaagcaat ttttcaacta tttatgaagg tggtggtaca agaaaagtat
     5581 cacctttttt ttgttataca aaaaaacagt tgacttttta tatgaacccc ttctataatg
     5641 ttactgaaat taagtaaaat gattgaacaa atgtaaagat aattgaaaag cttgattttg
     5701 gaggaaagca aatgaaaaaa attggagact taagattaat gatgaagtgt tgttcacttt
     5761 attacgaaga taatttgaat caacaggaaa tagcaaatca attgggaata tcaagaccaa
     5821 caatatcaag aatattaaaa gaagcttttg aacaaggaat agtaaaaata cagatagttg
     5881 atgttttgaa aaatgattac caaaaaatag agagaagttt ggagagaaaa tataaattaa
     5941 aagaagtaat agtagtagat gataaacagg atgcactaac acaaaaacaa gaactagcaa
     6001 gagctgtatc tgaatattta actagagtag taaaggaaaa tgatataata ggtgtttcaa
     6061 taggaacaac actaaaagaa ataccaagat atgtggaaaa aagtaactgt aaaaatgtta
     6121 catttatacc tctattagga gggattggag acaatgaaat agatattcat gcaaatcaaa
     6181 tagccgtaag tttagcgagg gcttttggag gtgattttaa attattacat gctccagctg
     6241 ttatgtctga cttgtctaca aaagagaaac tttgcaaaga cgaaaaaatt aaagaagtgc
     6301 tagatttaat agataaaaca actatagcaa ttgtgggtat aggaaatcct atgagtttaa
     6361 actcaacaat aatggcttct ggatacatga atgaagcaga tatagaagat ttaaaaaaat
     6421 acaactcaat aggtgcaatt tgtttacaag cctttgataa agaaggaaaa acttctatac
     6481 ttgaatttaa tcaaagagta ttaggagtga aactggaaga tttaaaaaag ataaaaagaa
     6541 caataggtgt cgcttcagga gatgaaaaaa tagaggcaat aaaggcatca ttaaaagcga
     6601 aatttataaa tagtttagca attaatcaca gtttagcact taaattatta gaagattgtg
     6661 actaatagaa agatagataa aagttaacag gagggaatgt atatgtcaat atcaaaagaa
     6721 acattgcttg aaatgtataa gagaatgaat caggctagaa aatttgaaga gaaagttagt
     6781 tggttttttg caagaggtat ggttcatgga acaactcact tatctgtagg tcaagaagcg
     6841 tcaagtgtgg cggctgtaat ggctttagaa aaaggagatt tagtatcatt aactcataga
     6901 ggacatagtc aatttatagg aatgggaata gacctaaata aaatgatggc tgaacttatg
     6961 ggtaaagaaa ctggattttg taagggaaaa ggaggctcaa tgcatatagc agatattgag
     7021 tcaggtaact taggagcaaa tggagttgta ggtggaggtc ttacaatagc tccaggagct
     7081 gcacttacac aacagtataa aaaaacaggc aaaatagttt tatgtagttt tggtgatgga
     7141 gcatcaaatg aaggtacttt tcatgaagga ataaatttat cttcaatatg gaaattgcca
     7201 ataatatttt actgtgagaa taatttatac ggtatgtcaa caagcataaa acgtcatatg
     7261 aatatagaaa gtattgccac tagagcagct tcttatggaa tagaaggaat atcaatagat
     7321 ggatataacc caatagaagt atacgaaaca gtacaaaaag ctgctgagaa gtgtagaaga
     7381 ggagaaggac ctgtattaat tgaaagtaga acttatagat ggttaggtca ttcaaaatca
     7441 gatgcaaatg tatatagaac aaaagaagaa atagaatcat ggaaagcaaa agaccctatt
     7501 gaatttttaa aaaattatct tatagaaaat aatctatcta atgaagatga attagataag
     7561 attcaagaat ttgcaaagca atcaatagaa gatgcagtag agtttgctca aaatagccca
     7621 aatccaaaaa tagagtcttt attagaggat gtatatgcag actaagaatg gtaaaggagg
     7681 aaaataaaaa tgagtacaag ggaattaaca tatgcacaag caataaagga agcaatgtca
     7741 gaagaaatga gaagagatga aaatgtaata tttatgggag aagatatagg aatatatgga
     7801 ggagcatttg gtgtatcagt tggtatgata gatgaatttg gaccagaaag agtaagggat
     7861 actcctatat cagaagcagc tatagcagga gcggcagcag gtgcagcagc aactggctta
     7921 agacctatta tggaagtaat gtttatggac tttgtaacaa tttctatgga tgcaattgta
     7981 aatcaagcgg caaaaatgag atatatgttt ggaggaaaag cacaagttcc aatggttgtt
     8041 agatgcccag gaggttctgg aactggttca gcagagcaac attcacaaag tttagaagct
     8101 tggttttgtc atgtaccagg agtaaaagta gttgcacctt ctacaccagc agatgctaag
     8161 ggattgttga aagcagctat aagagataat aatccagtca tatttgttga gaataaattg
     8221 ttatacagaa aaaaaggttt tgtgccagaa gatgattatg taattgaaat tggaaaggca
     8281 gatattaaaa gagagggtac tgatgttaca gtaattactt atggaagaat gttacaaagt
     8341 gttgaagaag cagcagagac tctatcaaaa gaaaatataa atgttgaaat tatagattta
     8401 agaactttat atccattaga taaagagact atagtaaaga gtgtatgtaa aactgggaga
     8461 gttttaattt gccatgaagc agctaagact ggtgggctag gtggagaaat atcagccctg
     8521 ataacagaaa gtgaatcatt tgattattta gatgctccag taaaaagaat ttgtggaaaa
     8581 gatgtgccaa taccatataa tccagagtta gaaaaagcag ttgtcccaag agttgatgaa
     8641 atagaagaag caataaaatc tttaattgtt agataaggag ggatagatat ggtggcaaat
     8701 aaaataaaag ccacaccagc agctagaagt caggcaagaa aagacaatat aaaattagat
     8761 aggctgattg gaagtggaga aaatggaagg attcatttag tagatgtgtt aaattattta
     8821 aaagataata aagcaaatac tactccttta gctagaagaa tagcagaaga tttaaatata
     8881 gatttagaaa ctattgtagg tactggatat aatggaaaga taagaaagtg tgatgtggaa
     8941 aaattaactg gaaaagaaac tattgtatca actaatacat ctaaatctag tgagaaaaaa
     9001 gagctgaaaa ttgaaaatga aaattcaaga atgtttaaca cagtagaagg aatatttgaa
     9061 aagccaaatc caatgagagc tactgtagca aaacgaatgt cagaaagtta ttttagtgct
     9121 cctgtattta cattcaatat agaagtagat gctacagaat tgaaggtact tagagcaaag
     9181 ttaatcgaca cagtaaaaga atcaactgga gtaaaattaa ctatgacaga tttaatagta
     9241 atggcagtat ctaaaatact tccaaatcat caagccttaa attcagcatg gacagatgag
     9301 ggtatattta gatataaaga tgtaaatata gctattgcag ttggtttaga tgaaggctta
     9361 tatgttccgg tagtaaaaaa tgcaaacaaa aagtctttaa aagaaatagc aaaagaaagc
     9421 aaagaacttg cggaaaaagt aaagacaggg aaacttatgc cagcagacca agaaggtaat
     9481 acatttacaa taagtaatgt tggaatgtat ggtataacta catttacacc tataataaat
     9541 atgccttcaa gtgcaatctt aggggtaggt gctacacaag ataagtttgt tccagtaaat
     9601 ggagaggcta aaataaaacc tataatgaac ttatcattaa catcagacca tagagtaata
     9661 gatggaacag tagctgcaaa attcttaaaa gatttaaaag aattgttgga aaatccttta
     9721 tcaatgcttg tataggtttt gatattttgt tactgatata aatagtttga atagattata
     9781 ttattataat tgtttcagta aatttaaagc ttttatatat taagaattat ttttaacagt
     9841 aactatatgt gaatatgatt tgcttaatta aaatataaga aaataaatag gaggagagcc
     9901 tatgtcagta gaagtaataa tgccaaaagc aggagtagcc atggaagaag gtactatagt
     9961 atcttggcta aagcaagaag gcgaagaagt aaagattgga gaacctatac tagaaataac
    10021 aacagataaa gttaatatgg agatagagtc agaaggagaa ggtacacttg ctgtaatcat
    10081 acataaagag gaaggtgaag tattaccagt atttacagta ataggtgtaa tagcagaaaa
    10141 aggggaaaat caagaggaag taaaagctaa atatttatct ggaaatgttt caaaagaaga
    10201 tatagttgaa gagaatcaaa atatagaatt taaagaagaa aaaataaaca aaaaagagtg
    10261 taatcatgat tatgatgtag tggtaatagg aggaggacct gggggatatt tatcagcatt
    10321 aaaagcagct cttttaggtg gaagagttgc tttagttgaa gaaaatatac taggaggtac
    10381 ttgcttaaat agaggttgta tccctacaaa gacttatata aaaacagcag aaatattaga
    10441 agaaattgac caattatcaa agagaggtgt aaaagtaact gtagataaag aacaggacat
    10501 taagaaggcc ataaaatata aaaatagagt agttaaaaaa cttacagctg gtgttggcgg
    10561 attattaaaa agtagagatg tagatgtgtt taatcttaaa gctagtgtta aagaagagca
    10621 taaagtaatt ttatcagatg gaaaagtttt agatacagaa aatataataa ttgctacagg
    10681 ttccaaagta aggatattac ctataaaagg aatagaatca aatttaataa taactagtac
    10741 agaagcatta gatttggaaa cagtgccaga agaattagta ataattggtg gtggagtgat
    10801 tggttgtgaa tttgcagaga tattcaattc aagaggttct aaagtaacta tagtagaaat
    10861 ggaagataga gtaatcccaa gaatggataa agagttaagc gaatctttaa aatattctct
    10921 tagtaaaaaa ggtataaatg tattaactaa aaagaaagtt tctgaattta aagaagaagg
    10981 aaataacata ttagtgtgca ttgaaggtga agagccaata aaagcagatt tatgtctata
    11041 tgctatagga agagaagcta atctttctgg catagaggac ttagatatta aaatagataa
    11101 aggttcaata gttgtaaata gtaaaatgga aacaagtatg ccaagcatat atgcagtagg
    11161 tgatgttact ggaggtgtaa tgttagctca tgctgctttt aaaatgggag aggtagcagc
    11221 ttcaaatgct ctaggtgtga ataaagaagt tgacttaggt gcattaccta gttgtgttta
    11281 tacaattcca gaagtagcat cagttggtat tacagaagag gatgctagaa aaaaatataa
    11341 tgtaaaagtt ggtaagttta attttgctgg aaatggaaga gctttagctt ctggtcaaga
    11401 acaaggatat gtgaaggtag tagcagatgc taaatatgga gaaattttag gtatacatat
    11461 gtttggatgt ggtgtagcag aacttataaa tcatgcagca tcatttaaag cgttagagat
    11521 acctactgat gaagcaagtg agttgatatt tggtcatcca tgtacttcag aagctttaat
    11581 ggaagctttg gcagatgtaa atggagaatg tttacactta cctaaaaaat aaatctaaac
    11641 ataaattatt aatttaagtc tattattaat attaatttat atcataaaat tttaaaacac
    11701 ttacttagcc atacccctta ttatgcaaat aatttatttg cataataagg ggtatttttt
    11761 atggaaaaat atagaatata gaatccttgt agtttttgtt gtacaaaaaa gatagtaata
    11821 taaaataaca ttatcatttt agaatgattt caattgtaat taggaaaagt gatttttttg
    11881 tttttaaaaa aagtattatt tgtaaaatat aaaaaaaaat ttcttaatat ttgaataaaa
    11941 gaatagctta atttaaatgt tattacttta taaaaaaata ataagtacaa aaaaaacaat
    12001 atatggcata taagttgcta tataaataat aaggagggaa caattataaa tgaggcaaag
    12061 aataaaaaac ttaattgaaa atgaagataa gaaaaatcct ttaacagatg aagtgatttc
    12121 aagtaggtta aatattcgaa gagaagatgt tacctttttg agaaatgagt taaaaataga
    12181 agattcaaga caaagaagaa aagttgtttt aattaaagct ataaaagata ttttaaaaga
    12241 agaaaaaact ataaataaaa atgatataac aagaagatta aacagcattg ggtttacagt
    12301 atcaagattt acagtcatac aatacttaaa agagattcaa gattctggtg acctaaaact
    12361 tgataaagag agtgatgata aaaaagaaga aataatcaat aatagagaaa ataaagatat
    12421 agcatttgat aaactcatag gtagtagtgg tagcttaagt acaattataa aactagccaa
    12481 agcagcaatt ctttatccac ctcatggtct tcatactatt attatggggc caactggtgt
    12541 tggaaagagt gagttagcag agtgtatgta taaatttgca gtagaatcat ccagattccc
    12601 taagaatgca ccatttatag tttttaatgc agcagattat tcagagaatc cacagctttt
    12661 gctatcacag ttatttggac acgttaaagg tgcttttaca ggagctgatg agaacaaaga
    12721 aggattagta tcaaaggcag atggaggaat attgtttata gatgaaatac atagattacc
    12781 tcatgaaggt caagaaatac tatttcaact gattgataaa ggtatggtca gaaagttagg
    12841 agaaacaaga ctaacacata aaatagatgt aatgattatt tcagcaactt ctgagccaat
    12901 agattctcat ttacttaata catttaaaag acgtatacct gtaacaattg aaataccaga
    12961 attagttgca agaccattaa atgagaggtt tgacattata aataatttct tcttaataga
    13021 agctcaaaga atgggagcaa acatacatat aaagtcagat gtattaaaag ctcttatgct
    13081 ttacgattgt ataggtaatg taggtcaact tagaagtgat attcaagtag cttgtgctag
    13141 gggactatta aaccaactta caaacaaact aaaagaagta aatataagca tatcagatgt
    13201 acctggatat gtaaaacaag gtcttataaa aatcagaaat tgtagaggca aaatagaaaa
    13261 ttatgtagat ggggatttaa ttatagattc aaatcttcaa ggtgagatag aaaagaaaaa
    13321 tgaagatata tatacttttc cagatgaaat atacaaactt actgaaagaa gacatattga
    13381 tttattggaa caaggtttag attttgatgt tataaataga atcattggag gagaattaga
    13441 aagttgcatt caaaaataca taaaacaagt taaaaaagtt tctattagta acaatcttcc
    13501 agatataagt ccaattgttg gaaaatatat cgtagagcta acttatgaag taattaaaat
    13561 agcatcacaa tacctcagtt cactagaacc gaatctacat ttatgtttag cagttcacct
    13621 aaaagcagct tttgagagat taaaagaagg aaaagtaata tcaaatgctc atttgaaaga
    13681 aataatagga aattatccag tagaatattc tgtagcagaa aaggtagctc aatatttaaa
    13741 ggaaagatat aacataaact taccaaaaga agaaataggt ataatagcaa tatattttaa
    13801 aatgagtagt agaagagata tgtctgaaca aaaaaagata ggcgtacttc ttatggctca
    13861 cggaagagta gcatcttctg tatgtgaggt tacaaataaa ctattaggaa ttaatcacgc
    13921 aaggtattta gatatgccat ttgagaagaa gccggaagaa atgattgaag aagctactac
    13981 aataataaaa gatattgatg aaggtagagg tgtgattata ttagtagata tgggttcact
    14041 ttcatatttt tgagatatta tttctggaaa gactggaatt aatataagga caatatctag
    14101 atttgataca ttgctttcta tagaggttat acgaagagca gttttaccag atttaacaat
    14161 agacgagata gttgatgaag ttaaaggtat agagccacaa gtaaaagaaa aggttagtaa
    14221 atgtaacaat acaaaagtag attttagaaa acctataatt ttaacattat gtattactgg
    14281 attaggggga gcttgtaaga taaaaaaaat cttagaggaa aatattaaaa gtataactga
    14341 aaaggttgat atagaaccta taggtatgat tgaatatcca agtcttgatt tagagataaa
    14401 acaaataagc aatgataaaa atgtaatcgc catagttgga acaattgacc ctcagatagc
    14461 aaatatccct ttttttagtc tagtagacat aaatgataaa gcaaagttaa atcaaataaa
    14521 taacctaatt gatacaaatc aaattggtga aacaaataaa aaatatgaat tatcagattt
    14581 aatacataaa gaagatatat ttgtggattt acaggttaaa tcaaaagaag aagttctaaa
    14641 atatatgagt aaaaaactta gagagaaagg ttatgtgaca aaaaatttct ataaaaaagt
    14701 agtggaaaga gataacttat ttccaactga attatcagat gaaatagcaa tacctcacac
    14761 agatggaata gatgtaatta ggccagctat aagtatagcc atcttaaaga agcctattgt
    14821 gtggagtaaa agtaaagtaa aaataatact tttattagct gtagaccaaa agtgttttaa
    14881 tccattaagc actctactct catttataga aacagatgaa tttaagaaga taaaaaacat
    14941 taaagataga gattttatta gggagatgat attagatgga gtcaatgaaa ataattagta
    15001 gcaatttaat ttttaaaaat attgaagtgt ctaacaatga agatgcatta aagtttttgg
    15061 gacaacgttt gtttgacgaa cagtatgtaa aagaaagcta catacaagct gttgtagcaa
    15121 gagaaaaaaa gtatgctaca gggttaccaa cagaaatata cggagtggca ataccacata
    15181 cagatatagt acatgtaaat gagccaggaa tagcaattgg aatactgaat aaacctgtaa
    15241 aatttataat gatgggtaca gacgatactg aaattgatgt aaaagttgta tttatgttag
    15301 cagtgaaaga gccacaagaa caattgcaac tattagaaaa acttatgact atatttcaag
    15361 ataagaatat actaaataat attgttgact taagtgaaga aagtgtgagt gatttattaa
    15421 actctaaatt gaaaaattaa acatgtaaag tatattaaaa atgtaaaatt aaacatgcaa
    15481 agtatgttaa aaaatataaa aagattatta aagatttcta actatagata tctttaaaat
    15541 aaaataattt caatgggggt gactaatgtg gctaaaaaga tattagttgc atgtggaaca
    15601 ggagtatgta cttcaacaat tgctataaac aaattaaaga aagcacttca agacattggt
    15661 aaattggata tggttaatat tacacaatgt aaagtagtag aagttgcatc aaaagcacct
    15721 gattatgatt taataatttg tactacacaa gtatctagta gtataaaaac accagtgata
    15781 aatggcttac cttttttaac tggtgtcgga atggataaat taatcaatga tgtattagaa
    15841 gagttagaat tataattaaa aaaattaagg aacatggtct ctaaaatcat aagttttaga
    15901 gacttaaaat taaataatgg agggtcttta tatggagata ttaaatttca ttgttggtct
    15961 aggtgcacaa gtaatgatgc caataataat atgtattttt ggtttgatat taggtacaaa
    16021 actaggaaaa tctttaagag ctggtttaac tgtgggagtt gggtttatag gtcttaacac
    16081 tattatagct ttgctaacag ataatttagg tccagctaca caacaaatgg ttaagaattt
    16141 agggctaagt ttatcaataa tagatgtagg atggccagca gcatcagcta tagcttttgc
    16201 atcaacagta ggggctttaa ttattccaat aggtcttgtg gtaaatatag ttatgctaat
    16261 tactaatact actcaaactg ttgatgtaga catatgggat tactggcatt ttgcttttac
    16321 aggagcttta gtggcagggg ctactcaaag tgtaatgtgg ggagtatttg cagcagtagc
    16381 aaatatggta attgtattgg taatggcaga tttaactgct ccaggtatag aggaatattt
    16441 aggtatgcca ggtatatctc ttcctcatgg atttacacag gcatttgttc caattgctat
    16501 agtagtaaat aaattattag acctaatacc aggcataaat aagattgaga taaatgctga
    16561 tacattgcag aaaaaatttg gattatttgg agaaccttta ataatggggt ctgtaatagg
    16621 tgtaataatt ggtattgctg cgaaatatga tataaaagga atattacaat taggtgtaac
    16681 tatgggagct gtattaatat taattccaaa gatggcagca ttattaatgg aaggattatt
    16741 accagtatca gaagctgcac aggaatttat tgagaagaga tttaaaaaca gaggcaagat
    16801 ttatataggc ttagattcag cagtaggaat aggtcatcct gttacattat cagttgcact
    16861 agtgttggtt cctttgacta tacttatagc agctatattg ccaggaaata aagttttacc
    16921 atttgcagat ttagcagtaa ttccatttgc acttgtatta atcgtaccaa ttacaaaggg
    16981 aaatgtgttt agaacattaa taataggaat aattataatt acttctggat tattaatcgc
    17041 aactaactta gctccattat ttacccaaat ggctttaaat gctagtttta aaatgcctga
    17101 aggagcaact atgatttcaa gtatttgtga tggtgcgaat ccattaagtt gggtatttgt
    17161 aaaagttatg aactataaag ttattggagg agtagttttt ggagttatag ctttaggaat
    17221 ggctatttac aataggaata gaataataaa tgaaaataaa aagctatcta tagaagaata
    17281 aggctaaaac aattttatta aggagtgtat aggttatgaa aaaatataaa tatgattcta
    17341 aaagatactt actcagtgtt acaataccag gattttttat gattttaata ttgatttatg
    17401 cactgtttaa taactataca aattttaatt tgaatatata tagtttgtta ataattgtct
    17461 gtacttatgg tatattcaat acttttatat catcaagtaa tcctaaaaaa attattattg
    17521 atagcaaatg tatacatttt acctcattta tgactacaca taagtatgaa attaaagata
    17581 tagaaaagat aagaataaga gaattttata ataaaaaaat ttatgtaaaa attaatgatg
    17641 gaaacctttt taaaggtagg tattggataa gaacaaatat gtttaataat agtatagcat
    17701 tatatagcta tttcattgag ttggaagagt gcttatatcc agattcacta aaatttagaa
    17761 ataaaaaatt tgagaataag aacatataaa aataaaaaat tgaattgaag cctagcttaa
    17821 tcttttttga taagatataa gtttgaactt tatactaaat ttttagaatt ttcaatcaag
    17881 acaataacat aatagctaag caattagaac acaattaaga tgtataaggc atgtatatga
    17941 tgaatgggag gatttaatta atgttattag agaaattaag aaaagaagta ttacaagcat
    18001 ctttagacct tttaaactat aatttagtga cattaacagg aggaaatgta agtgggagag
    18061 atgaacaaac tggctatata gcaatcaccc caagtggaat ggattataga aatcttacac
    18121 caagtgatat tgttatagta gatgtagatg gaaatattat agatggaaaa tggaaagcat
    18181 cagtggattt atcagaccac ttgtatatat ataagcatag agaagacata aatagtataa
    18241 ttcatacaca ttctacatac tcaagttgtt ttgctatttt aaatgaacct atagaatgtg
    18301 caagtacaac tcttgcaaat gaagtagggg gttcagtacc agttgcaaaa ttttcaccac
    18361 caacatctaa aaagatggga aaatgtgtta ttgaagcaat tggtgacaaa agagcatgtc
    18421 ttttagcaaa tcatggcgta attgcagttg gtccatctgt aggacatgct cttacagccg
    18481 ctgtaatgtt agaagattca gcaaaagtat attacttagc aaaatcaatt ggcacaccag
    18541 tgctactgcc tgatgaagaa attcaaagag cgagagatgt attttttaat gtctatggtc
    18601 aggataaata aattagataa aattttaaaa gtaatatgaa aatatagata ttcaatgata
    18661 ttaaggagat ttataatgat ataataagtt taatagttaa gaagtaaaat atgaaatgta
    18721 atagtatata aattcattta agactaaaaa gaataagata aaataaaaag gagtctgtat
    18781 ttctatgtat tacttattaa acaaaagcac aaatccttac tttaatttgg cattagaaga
    18841 atatctattt ttaaatgata aatataatga tgatattata atcatatgga gaaatgaaga
    18901 atccattttt ataggtaaaa atcaaaatcc atatcaggaa gtatattatg atgttattga
    18961 aaagggagaa attcctattt taagaagaat atctggagga ggaactgttt atcatgactt
    19021 aggtaatatc aatatgtcat tcatacaaaa aaataggcaa cttcatgaga tagatttttt
    19081 ggagcacact aagtttatgc aaaagatgct ttcaacctta ggactagatg tatctataac
    19141 tgaaagaaaa gatttatttt taaatgggaa aaaaatttca ggttctgcac agtctataaa
    19201 aagaaaaaat tctctttatc atggaactct tctttacgat tcagatttaa ataaattaac
    19261 taaatattta aattcaaata aagcaactga atcaaatgca actaaatctg tttcaagtaa
    19321 agttacaaat attaagtttt tactcgaaaa agatataaat tactttttag attattgtgt
    19381 tgaatattta aaatctaata taaagaatat aaaagaactt gaattggatg aagaagatat
    19441 taaaagtgta tacaatatag gagaagataa atataaaaag atagagtgga catatggtaa
    19501 aacgccgaaa tttcaagtag tgctaccatt taataatgaa agtaaaataa atatacatgt
    19561 taacagatgg aaagtatcaa aattttttat ttcttatgat gataatgtaa ttaatatgga
    19621 caagttgtta gatttgaatt tctttaaaga agaaataaaa gagagtattt tagaaaatta
    19681 tccacattac atagatttaa tcaatttaat attttaatat aaaacctcta atttgataaa
    19741 attgtcttat atcaaactag aggtttttaa tattcttgca tattaacaac cattacaaaa
    19801 ttgtaaaatt aaaaacaaca ctattgaaaa atcaatatat attgtaataa atatatgtgt
    19861 ttttcattag caaatagaca ttaactattt atacaactga aaatttaata tataattaat
    19921 cagatggtta aaataaaaat attaaaagtt ttaataaatg atggttaaaa ataaaatacg
    19981 aggtgatacc atgtatagtg ttataattgt tgcagcggga agcggaagga gaatgaactt
    20041 agatataaac aaacagttca taaaattaag agaaaaagaa attattgctc atacaattca
    20101 agttttttat gaaaatataa atatagatga aatagtggta tgtataaaaa aagaagaaga
    20161 agattttttt aaagaaaata taataaataa gtataacttt aaaaatataa aaatagcata
    20221 tggcggaaaa gaaagacagg actcaatata taatggttta aaaaaattag atgaaaattg
    20281 tgatatagta cttatccatg atggagcaag accatttgtc gaccatagaa taatcaatga
    20341 atctataaag gtagctaaag aaaaaaaggc tgtggttgta ggtgttccag taagtgatac
    20401 tataaagata gtgtcagatg gaacagtaca agaaactcca gaaagaaatt tgctgtgggc
    20461 agcacaaacc cctcaaacct ttgaatataa tctcataata gatgcttatg aacaagcata
    20521 taagaacaat tattatggta ctgatgattc aatgttggta gaaaatatag gtcaaagtgt
    20581 cactatggta atgggttctt atgaaaacat aaaaataact agtccagaag atttaaatat
    20641 agcagaacaa atattaaata tggaaaaaag agatgaagtt aacagtagaa ggagaattat
    20701 atgagaatag gattaggata tgatgtacac aaattaacag aggatagaaa acttataatc
    20761 ggtggtgtag agataccaca cgataaaggt ttgttgggtc attctgatgc tgatgtattg
    20821 atacatgcaa taatggattc tattttaggt gctctagcat taggagatat agggaagcat
    20881 ttcccagata cagatgaaga atataaggga gcagatagta tgaaattatt agaacatgtg
    20941 tataatctaa taacaagtaa aggatataaa attggtaata tagacagtac tataattgct
    21001 caaagcccta agatggctcc atatattgag agtatgagaa gtaatatatc aaaagtttta
    21061 aacacagaca ttgataatat aaacataaag gcaacaacag aagaaggtct tggttttaca
    21121 ggagcaaaac agggaatagc ttcacaaagt atttgtttat tattgttgac aagtcagaat
    21181 aattaatata ttattagtaa taagtttaat ggatatcaag taataggcct atatagaata
    21241 tctataatgt atgaatttaa gtgaaattaa tataaatata aaattagcta tgataaagaa
    21301 atagtaaaat gatgaaatct tcacagagag aaaacggtgg tgagagtttt caagaggaag
    21361 tctttgaacc tgtcttttag cttaaaggta taaaaatctt tacggtagga accgttatat
    21421 tcccaaaaga gagctattca tatagctaac tagagtggta ccgcggaaat taaacctttc
    21481 gtctctatac ttgtatagag atgagaggtt ttttatattt tcaggaagtt aatattcagg
    21541 aggaatctat taatgaaaat gtcaaaaatg tttatgccga cattaaaaga aatcccagca
    21601 gatgctgaga ttacaagcca tcaattaatg gttaggtcag gcatgataaa aaaaatgact
    21661 tcaggtgttt ataaccaatt accaatgggg ttaagagtgt tcaaaaaaat tgaacaaata
    21721 attagagaag aattaaataa aaaagattgt caagaaatac tttgtgcagc cttacttcct
    21781 tctgaattat ggaaagagtc tggaaggtgg actgctatgg gtgaagaaat gtttagacta
    21841 aaagacagaa cagaaagaga gtactgtctt gggccaacac atgaagaagc attcactgat
    21901 ataataagac aagaaattac ttcatataaa caattgccac taaacttgta ccaaatacaa
    21961 gtaaaatata gagacgagag aagaccaaga tttggtgtta tgagaactaa gacttttaca
    22021 atgaaggatg cctatagttt tgatgtagat gataaaggtt tagataagtc atatcaagat
    22081 atgtttgatg catatgtaag tatatttgat agatgtggat tggagaatag ccctgtacag
    22141 gcagattcag gagctatagg aggttctaca tcagccgaat ttatggttaa gtcagaagtt
    22201 ggtgaagatg aagttgtatt ttgtagtgga tgtgattatg cagccaatgt agaaagagca
    22261 gaatcttgta atctagcatc acaaaaagaa gaaatgaaag aattagaaga ggttcataca
    22321 ccaggagcag ctacaataaa agagttagaa gaatttttaa aaacttctcc agataagttt
    22381 gctaaaactt tagtatatga ggcagatgga aaaacagttg tagtcgttgt aagaggagat
    22441 agagaagtta atgaaatcaa agtatctaat gccataggtt ctgttataga atttgctctt
    22501 gctacagatg atgtagtgag aaaagtaact aatgcagaag taggatttgc aggtccaata
    22561 ggtataaatg ctgactatgt atttatagat aaagaaattg ttgaacaaag aaacatagtt
    22621 gttggggcta ataaaactga gtaccatata aaaaatgcta actatggaag agattttgaa
    22681 ggaatagttg gagattttag aaatgttcaa gaaggcgaca agtgtatagt atgtggtaag
    22741 ccacttgaaa tagctagagg tgttgaagta ggacatatat ttaaactagg aactaagtac
    22801 tcagaatcta tgaatgcaaa cttcatagac aaagatggaa aatcaaagcc tatagtaatg
    22861 ggatgttatg gaataggtgt tgaaagaaca gcagcagcta taattgaaca acataatgat
    22921 gagaaaggta taatatggcc tttgtcagta gctccatacc atgttgtgat agttccagca
    22981 aatatgaaaa atgaagaaca aatatcaata gctgaaaata tatataatga tttgcaagct
    23041 atgggtgtag aagttttatt agatgacaga gatgaaagaa taggtgtcaa attcaatgat
    23101 tcagaattaa taggaatacc tatgaggatt acagtaggta aaaatattaa tgaaggtaaa
    23161 gtagaattta aacttagaca taaagaagat aaagagataa ttgacataga agaaataaat
    23221 gaaaaagtaa aggcagagtt tatcaggaat aatgttaggt taggataata acttaaataa
    23281 gatattgtat agataatcaa agttgtactt tatagacacc attttatcta tatataagga
    23341 tataaaatgg tgttttttta taaaacaaat aaatgaggag gtaattgaaa tggcaaaaaa
    23401 tgaaaaacaa tttgttgaag aaataacaaa gatggaagat gattttccac aatggtatac
    23461 agatgtaata actaagactg acttagttga ttatgcacca gtaaaaggtt ttatggttgt
    23521 aaagccatat ggatatgcat tatgggaaaa gatgcaagaa tttatggata aaaaatttaa
    23581 agaaactgga cataaaaact gttattttcc attattaata ccagagagtt tattaaacaa
    23641 agaagcagaa cacgttgaag gttttgctcc agaagttgca tgggtaacac atggaggaaa
    23701 taaaaaatta gaagaaagat tatgtgtaag acctacttca gagactataa tatgtacaat
    23761 gtatgcgaaa tggttaaaat catatagaga attaccatat ctttataatc aatggtgctc
    23821 agttgttaga tgggaaaaat caactagacc tttcttaaga acatctgagt ttttatggca
    23881 agaaggtcat acgttacatg aaacagcaga agaagcacaa gaagaaacaa tacaacaatt
    23941 agaagtatat aaagcgctat gtgaagaatt acttgcaatg ccagttgtag ctggtcaaaa
    24001 aagtgaaagt gagaaatttg caggtggtga aagaacatat actatagaag ctatgatgca
    24061 tgatggtaaa gcattacaat caggtacaag tcacttccta ggtcaacact ttactaaggc
    24121 ttttgatatt acttttgcag atagggaagg aaatttagct aatccatacc atacatcttg
    24181 gggagcatct acaagactta taggtggact tataatgact catagtgata acagaggttt
    24241 agtattacca ccaagagtag ctccaattca agttgttata gttcctatag ctgctaaaaa
    24301 aggaaatgta atggagactg ttgataaaat atatgctgat ttaaaagcta agggagtagc
    24361 tgtagaagtt gatgatagag ataactatac aactggatgg aaatttaatg agtgggaaat
    24421 gaaaggtgtt ccagtaagag ttgaaattgg accaaaagat atagaaaata atcaagctat
    24481 ggtatttaga agagatactt tagaaaagga ttctatgcct ttagaaggat tagctgatgc
    24541 tatatgtgat ttatttgatg ttatacacaa tgatatgttt gaaaaagcaa gaaaacatag
    24601 agaagacaat acatctatag ttgaaaatat ggatgagttt agaaaagctt tagaagaaaa
    24661 gccaggattc ataaagacta tgtggtgtgg agatgcagaa tgtgaagcta agattaagga
    24721 agaaacagga gcaactataa gatgcttacc ttttgaacaa gaaaacttag gtcacaagtg
    24781 tgtgtactgt ggaaaagaag cagatagtat ggtagtaatg gcaaaagctt actaaaatac
    24841 atttaaataa aaactggggt agaaagtatt taatacttta tcccagtttt ttgtttttta
    24901 tgagtaataa aatgtatttg taactatgtc ataagataag tcatagatac ttatattaaa
    24961 aagaatataa ta
//
LOCUS       DAFKMX010000039        24557 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFKMX010000039 DAFKMX010000000
VERSION     DAFKMX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.039"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            60..1937
                     /locus_tag="LZB08_001296"
     CDS             60..1937
                     /locus_tag="LZB08_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7314806.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2179..4092
                     /locus_tag="LZB08_001297"
     CDS             2179..4092
                     /locus_tag="LZB08_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314807.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSVFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     SKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKE
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDDIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4085..6304
                     /locus_tag="LZB08_001298"
     CDS             4085..6304
                     /locus_tag="LZB08_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBM7314808.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6413..7243
                     /locus_tag="LZB08_001299"
     CDS             6413..7243
                     /locus_tag="LZB08_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBM7314809.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7233..10700
                     /gene="addB"
                     /locus_tag="LZB08_001300"
     CDS             7233..10700
                     /gene="addB"
                     /locus_tag="LZB08_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBM7314810.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRISKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRY
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10700..14527
                     /gene="addA"
                     /locus_tag="LZB08_001301"
     CDS             10700..14527
                     /gene="addA"
                     /locus_tag="LZB08_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBM7314811.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14573..15784
                     /locus_tag="LZB08_001302"
     CDS             14573..15784
                     /locus_tag="LZB08_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBM7314812.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15771..19298
                     /locus_tag="LZB08_001303"
     CDS             15771..19298
                     /locus_tag="LZB08_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7314813.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDELEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19429..20682
                     /locus_tag="LZB08_001304"
     CDS             19429..20682
                     /locus_tag="LZB08_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBM7314814.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20831..21883
                     /gene="ytvI"
                     /locus_tag="LZB08_001305"
     CDS             20831..21883
                     /gene="ytvI"
                     /locus_tag="LZB08_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBM7314815.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            21974..23200
                     /gene="pepT"
                     /locus_tag="LZB08_001306"
     CDS             21974..23200
                     /gene="pepT"
                     /locus_tag="LZB08_001306"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBM7314816.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGMDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23407..24459
                     /locus_tag="LZB08_001307"
     CDS             23407..24459
                     /locus_tag="LZB08_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBM7314817.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        10486 a         2350 c         4384 g         7337 t
ORIGIN      
        1 ctgaatctaa tgctatggaa aataacgctg atttaaatat aaacttagag aaaaaaagta
       61 tcgttttagg tagcaaatca aaagttagtg tcaaatttaa agaaaaacca gatgcagata
      121 gcattacatt aaagtataaa tgctatgaca tgccattgaa tacaactcta aattacaatc
      181 aatcaactgg ggcatatgaa ggaactatca attataacca agacccagaa tatctaaatg
      241 tttgggaact acaagggata acaataaaca gcaaaaataa tcctaaaact ttaaacagac
      301 aagacctaga aaagctggga ttaaatttaa aagactataa tgtaacacag gaatgtataa
      361 ttgaagatat aacttctaga aaagatgtaa ataaatattt gagaaaaact tcttcaccta
      421 ttacagaact tacaggaagt gatagatatg aaacagcagt taaaataagt aaagagggct
      481 ggaaaaatgg ttcagataaa gtagttataa taaatggaga tgtaagtata gatggcatta
      541 tatcaactcc actggcaacc acatataatg caccaatact tttggttgaa aaaaacaatg
      601 tgcctaatag tgtaaaatca gaattaaagc gcctaaaccc taaagatata attataattg
      661 gagatgagaa tgctatttct aaaactactg ctaatcaaat taaatcaact gtaaatgcta
      721 gtcaaacacg tttaaatggt tctaatagat atgagacatc tttattgata gcaaaggaaa
      781 tagataaaaa tcatgatgtg gaaaaagtat acataacaaa tgctaatggc ggagaagtgg
      841 atgcacttac tatagcagca aaagcaggtc aagacaagca accaattata ttaactgata
      901 aagatagtat tacagacaat acatataaat ggttaaagag tgaggattta caaaatgctt
      961 attttatagg tggtcctcaa atgatatcaa caaatgttat aaataaggta aatggaataa
     1021 ctaaagatag tgttactaat aatagagtat acggagcaga tagacacgaa acaaatgcaa
     1081 acgtaataaa aaaattctat acagatgatg agttagaggc tgttttagta gctaaatcag
     1141 atgtacttgt tgatgcttta gcagcaggtc cattggctgc gaacttaaaa tctccaatac
     1201 ttataacacc aaagacgtat gtatctgcat accataaaga taatttagaa gctaaatcag
     1261 ctaataaggt atacaaaata ggaggaggat tgacttctaa ggtaatgagc tctatagcat
     1321 catcattatc taaacacaat acgactccaa cagaaccagg aaatagtggg ggcaagacag
     1381 ttatgattga cccagggcat ggtggttcag cacctggaaa ttcatctgga ggaatgattg
     1441 aaaaagatta caatttaaat acttcacttg caacaactga atatttacgt tcaaagggat
     1501 tcaatgtaat aatgacaaga gacacagata agactttatc tcttggaaat agaactgctc
     1561 tatctaattc attgaaacca gatttattta caagtataca ttataatggc tcaactaata
     1621 aacaaggtca tggtgtagaa gtattttata agcttaaaga taaaaatgga gggactacta
     1681 aaactgtagc taccaatata ttaaatagaa ttttagagaa atttaaactt acaaatagag
     1741 gtataaaaac aagagtactt cctagtgatt ctacaaaaga ttatttatat gttttaagaa
     1801 gtaatgatat gccagctgta cttgtagaat gtgcattttt ggataatgaa aatgatatga
     1861 gtttaataaa ctcatctgca aaagtaaaag aaatgggtac acaaataggt aaaggaatag
     1921 aagattcatt aaaataaatg gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt
     1981 gtaaatttat actaaagtca aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt
     2041 acatttgcac atgtgaatgc aagtatatat acagtctttt tttgtagtta aatgcaataa
     2101 tttgattgtg ttttgtaaaa tacacaatat gatataatta gataatatac acttaattta
     2161 attgaggaag gtgattatat ggaatattta tttgaaaaaa gtagtttaaa agaatatgga
     2221 aatgaagctt tagataaata tagagaatta ttagataaaa ctggtaataa ttcaaggtgt
     2281 attcttttgt tagtaccaaa caataacaca aagatacgat atgaaagggc tctaagatta
     2341 aattatagtg aagaactgaa aataacaaca tatataagtt ttgtgaagaa agaattagtt
     2401 aaatactggc cactaataat agaaaaatgt gatggaataa gtacaaaggt tgtatctcca
     2461 acatttatat caaatagttt aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg
     2521 atacaagaag gatattttga agatattaca ggaaccaata aatccatagc aaatagtata
     2581 atgactaaca taaataagtc tgtatttaat ttaattgatt ttgattttat tggagaaaaa
     2641 atatattcat caaagaaaaa taaagatagt atatatagat tttcatacac tcaaatggat
     2701 gaaattatat catattatat aaattcctta ctatcaaatt caattttaga taatgcatta
     2761 agcatatatt tatacaatca atacctactt aatgatgatt tttatttaaa aaacttattt
     2821 acagaagtga gatatataat tgtggatagc cttgaaagct caagtaatgc agaagttgat
     2881 tttataacag aagcattgga taatacatta caaagttata tatattttga ttatagtagg
     2941 gattattctg tatttaataa tgtggatata gactatataa atgagaaaat aatatcaaaa
     3001 attaagtcta aagaagaaaa tattaaaaag aaggagatta gaatagaaga tttatactta
     3061 ttacctgcaa atatagaatt aaatcaatca agtcaattat ataatgaaat gttagattta
     3121 ataagtgaaa aagttatatc tcttatagaa gaagatgtat ctccaagaga tatagcaatt
     3181 atatctccta taaacaatag tatattggag catcaaatta gagattcttt gatagaaaaa
     3241 aatatagatg tttttagtac taagaaggat aaaaaagctg tggattatcc atatgggaat
     3301 gcattggtag tggctacatg tatattttat ggatatttag attttataaa agaggatgaa
     3361 tacttaagct ttttagaaac tcttttagaa ataaatagga taaaagcatt taagattttt
     3421 aatgaaacaa gacatttaag tattgatgat atagatgaac atagtcagta tcgtgagatt
     3481 ttacagtata ttgaagaaaa aaagaagagt gatataaaaa tacatgaatt tttaactcaa
     3541 ttttacatag ataaaatgtt aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa
     3601 aagataatag ctgaaagtga gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa
     3661 gaaaagatat ttgtagaagc tttaaaaagt acaataaacg attattactc tgttgtcgat
     3721 atagaagagt tgaaatccaa agacaaaata gtaataacta ctccttattc atatatatca
     3781 tcaaatatag atagaagtat acaaatatgg gtagatatag gaagtaatgc ttggaatatg
     3841 aaaatagaaa aagatataag taatttaatt gtacttagaa aatcttttga agaaaaaaag
     3901 atttatacaa atgaaatgga agaatattat aaaaaatact atctatacaa tacagtatat
     3961 aatttgttat taaatgcaaa gaaaatctat gcatttaaaa gtgaatatgc gattaatggt
     4021 tatatccaag agagtatatt atatagtata cttttaaaaa tctcacataa aggagataaa
     4081 aattatgatt aatataaatt atagagaaga ccagatacca ataataaatt acgataatgg
     4141 tactatggca gtaccagcag taccaggtgc tggaaaaaca tttataataa caaatttagt
     4201 agctaaacta ttacttgaac agaaacataa gggtggtaaa atattaattc ttacatatat
     4261 gaatagtgct gtaaataatt ttaaaggtag aataagaaag attcttgaag agaataaaat
     4321 agatgatact aattcatatg aagtaatgac tatacacagt ttggctgtaa aaataataaa
     4381 ggaaaagcca gaagttgtaa tgttaagtga ggattttaac atagctgacg atttgcaaaa
     4441 aagtattata ttaaatgaat gtataagtag atttagattt gaaggtggag aaagagcttt
     4501 tagatggttt ttaaaggagc aaaaagatga acgctggaaa gaaataactc ttgaagcctg
     4561 ggaaagaggt ttttttgaat ttgcaggaaa tgcaataagt gaacttaagt ataagggaat
     4621 ttctccagat aaacttgaag aaatattgtc aagaggacac aaaggaatgc ttaaaattat
     4681 tttaccaata tataaattgt atgatagaaa attaaagcaa aatggattgc tagattatga
     4741 tgatatatta attttagcag aaaaaacttt aagcttagac gaagggctta gaaagaagtt
     4801 tcaaactaga tacaaatata tatttgaaga tgagtgtcag gactcaaatg aaattcaagg
     4861 taatataata aaaattatat ctagcgaaaa taaaaaccta gttagagttg gagatataaa
     4921 tcagagtata acaggaacat tttcatcatc agaccctaaa ttttttaaag aatttataaa
     4981 tagtgcagat ttctgttata ggatggatat gtcaaatcga agttcaaaag atatacttga
     5041 tttagcaaac actcttgtca agtatgttac aaaggaattt agacagaaag aatgtagaca
     5101 tgctcttgaa gatatgcaaa taaaaactgt gccaaatgga atgggatata aagaaaaccc
     5161 taatccagaa cattataata taaatgtaaa gtggtatgag agttggaaaa ttgaaataga
     5221 gcaaactgct agatatgtta agggtataaa aaataaatat ccagataaaa gtataggtat
     5281 attggttcca tttaatgaac aagtgacaca agttgctaaa gaacttagag agaataatct
     5341 aacctttgaa gaattaggac caaattcttt gaataaaagg aaggttataa acaacatagc
     5401 atatattatt gattttatgt tgcattgtga tgatgtagaa aagttaatag ttgtacttaa
     5461 taaagttttt atagatactg ataatgaaga agaaataaag tattttttag aagaattaaa
     5521 aaactattct accgaagaaa ttatttataa ttttgaattt agtaagttag ataatgattt
     5581 aatagaattt aaagaagaaa gtgatatata cttaagtttt aaaagaggaa tagatattat
     5641 aaaacagata ttagaatatc caataataag gttagacctc ttagttttgt ttattgggaa
     5701 aaaacttaat ttagaaaaag aagataaagc tgtactggat tatatatctt tttatataaa
     5761 gtatctagta tcagaaaata taaatatgga tttagaagat gtatataatt tattgtttga
     5821 tgtaaagaac agggtgttta atcatataat agatgtggta tatgagataa atggatatga
     5881 accagagccc ggtagtataa cactatgtaa ctatcataaa tcaaagggga tggaatggga
     5941 ttgtgtgttt ttactaggtc ttgttgaata taatttccct gacaatatta atcaaaagtt
     6001 tcagagtgat aaatggtatc taaaagaaaa atataaaaac cctatggcaa ttattaaatc
     6061 agaagttgaa gctatattaa aaggaagtat ttcaacagat tatgctcata aaacgaagat
     6121 tgattctata aatgaaaaga ttagactttt atatgtaggg attactagag caaaggaaat
     6181 gttagtactt tcaggaagtg cttatagaga tgaaagtgat atagggaata agagaaaaga
     6241 gcaaaaacca tgtatatatt taagcagact taatcaacat attattgaaa aaaggtcaaa
     6301 ttaatatata ataagttatt aaaagattgc ttgaaatata tctaaaaggg gataaaacaa
     6361 tatgtacagt aagtaactac taaaaaataa agttaagaga gaagtgatat gagtgaacga
     6421 taaattaaaa tatttttctt atagtcaaaa ttcaattaac acatataaat catgtccatt
     6481 gaaatttaaa tataaatata ttgatagaat aaattggaaa aatgatgatg ttggtagtag
     6541 agagtattat gaaactttaa aaacaggaag agattttcat ttgatttgtg aaagatattt
     6601 tagcaatata cctcttggaa tctattttaa tgaggatgat aaaaatagta aaaagttttt
     6661 aaagtggatg gaaaatataa aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc
     6721 agaatatgaa gtaaggatga cattaaatgg ggatataata caggcaaagt atgatttagt
     6781 tgtaattgga aataacaata tagaaatatg ggattggaaa accgagtcta aaaagattga
     6841 ctataaacac gtagaaaata gaatacaaac tattgtttat atgtttttag caaaagaagt
     6901 aatacctaaa gttttaaaaa tggacataaa tgttaaagat ataaatatga gatattatca
     6961 acctgaattt gatgacttac caattaccat tttatatgat gaagaaaaac atgaagcaaa
     7021 tagaaataaa attcaaaatt atataagtat gataaaaaac actaattatg aagaacatac
     7081 atacgaagac aatctataca atgatataga aaaggtatat aataaagaag aacgtatgtg
     7141 ctatagaaat aaaaagcatt gcaagtattg tgaatttaat aagttatgta atggcaaaga
     7201 aatagattat agtattttgg aggcggaaat ttatgggact taggtttgta ttaggtagaa
     7261 gtggaagtgg aaaatccacc tatatactag atgagattaa aaaagaagct caaaaaaatg
     7321 aaacgacatc aattatactt ttagttccag aacagtacac atttgaggct gaaaatagaa
     7381 taagtaaact atttttagga aaagaaaaag ataagtatct aagagttagg gtacttagct
     7441 ttaagacact aagtaatata gttttttctc aagtaggagg attgacagat gtaaatataa
     7501 attctagtgg aaaagcaatg atggtatata gagctattga agatgtaagt gaagaattaa
     7561 atgtgttttc aaaatcaaaa tcgcagtctg gttttgtaag ctcaattaca gatatgatat
     7621 cagaaatgaa acaatacaat ataagccctg aaatgttaga aaatatatct ggagaattgg
     7681 ataatgaaac tttaagttta aaattaaaag atataagtaa aatatataat tcttttgaag
     7741 ggaaattgca tgaaaattat gtagatgctc aagatatgtt aacttcattg gcaagtaaaa
     7801 ttgaattaag cagttattta gatggagctt gtgtttatat agatgaattt acaggtttta
     7861 caccaaatca gtacaatgtc ataaagagta tattaaataa gtctaaaagt gttaatatct
     7921 ctttgactgt agatgatatc aactatattg ggtacagtaa atctgatatg ttttccagaa
     7981 cgaaatttac ttattcaaaa ttgactcaat tgtgcaacga agaaggcata aaaatattgc
     8041 cacaagtaaa tttaaacaca ggtgttataa aaagatttga aaaagttaag gaattacagc
     8101 acttagaaag attttataat gcttatccat ataaaatata ttcaaatcca actgaaaata
     8161 taaagataaa agagtttaac aatttatatt cagaggtgga agagatagcc agagaaattg
     8221 tacatttagt tagagataag aatgtaagat atagagatat aaccattgct acaagagatt
     8281 taaatagata tgatttttta gtacactcta tatttaatga atataatata ccaaacttta
     8341 tagataagaa gagagaggca aaaagtaatc caatagttat tttaattatt tcagctttag
     8401 aaatgaagaa tagacgatat gggtatgaaa ctatgtttag gtatttaaaa agtggactta
     8461 taggaattga taatgatgat ataaatttac tagaaaatta tgtgttggct aatgggatta
     8521 aaggtaaaaa atggtttgat gaaaaatggg attatagaat aacccaaagt ctttcaggtc
     8581 aagaaagtga atttgaactg gaattaaaag agaaaataaa tgaaataaaa aatagagtat
     8641 tagagcctat agtaatttta caagaaaaat taagaggaaa aaatagagta aaagaaatct
     8701 gtagatatat atatgaattt ttattagata taaatatgcc agaaactata gaaagtttaa
     8761 tagtaaattt taaggataaa ggggaacttg atgttgcaaa tcaatactca caagtttggg
     8821 acatagtagt ggacatatta gaccaaatgg tggaacttat gggtgatgaa ataatttctt
     8881 tagaaaaatt tataaaatta ataaccttag gttttgatga atatgaatta ggactggttc
     8941 ctcctagtat agaccaggta cttgtaagta gtgtggatag aatgaaaaat ccagacacga
     9001 aatatttata cctagttgga actactgatg gtgtgtttcc gctgattaca aaggatagtg
     9061 gaatattgag tgataatgat agggaaagtc ttggaaacaa aggaatcgaa gttgatattg
     9121 atagtaagac taggagtttt gaagaacagt ttcttgtata taaagctctt acttcgacat
     9181 ctaagaattt aaccataaca tatccaatat cagaccatga aggtaaaacg cttagaccat
     9241 caataattat atcaagactt aagaagatat ttccaaatat agaaaataaa agttatttag
     9301 tggaagaaaa taaaagcact gataaagata tacttaaaaa aataacggtt aaatccccta
     9361 catttaatga acttataaac gtaataaaaa attatgacag tgatggttac aatacagaag
     9421 agattaatag tatttggtta gatatatata ggtactattt aaaagatgaa atctatagtt
     9481 ctataacaaa aaaagttata aaaggtctta gttatactaa tcaagttcat aagattgaag
     9541 agaaaaaaat aaggtcattg tataaaagta attctcttag catatctagg cttgaaaaat
     9601 atgctgaatg tccatttgct tattttatac aatatggtct aaaagcaaaa aagcgtaaag
     9661 agtattcatt tacaccacca gatttaggaa cttttattca taatatttta gataggtttt
     9721 caaaagaact tttacaagat aatttaactt ggagagatat tgatgaaaaa tatatagaat
     9781 taaagatagg tataatagtt gatgaaataa ttttaaagat accaggatat atattaaata
     9841 gttctgagag gtataaatac cttgcatata gattaaagaa tatgttgaca actgctataa
     9901 ctataattag tcagcaaata aaacaaggtt catttgaacc aatagattat gaagttaaat
     9961 ttggcgacaa tggaaaatat ccacctataa aaatggtgtt agaaaatgga caagaagtaa
    10021 gtttaattgg acaaatagat agagttgatg aatttgaaga aggagaaaat aaatatataa
    10081 gaataataga ttataagtca ggcaataagt ccataagtct aacagaaata tattatggtc
    10141 tacaattaca attacttgtg tatttagatg caatccttga aagtgcaaag gatgaagata
    10201 taaatataaa tccagctgca atactatatt gtagaataaa taatccaata gcaaaattta
    10261 atgaagataa agatgatgaa gaaatacaag aagcaatatt aaaagaactt agaatgaagg
    10321 ggcttgtagt aaaagactct cacatagtta aagagatgga taaatcacta atagatggag
    10381 aaaggaaaaa ctctttagta ataccagtag gtcttactaa ggatggaaat gtaggtaaaa
    10441 gtacttcagc aataagctat gaggatttca aactgcttag aaaatatgta aggtatgcta
    10501 taaaggattt atgtgaagaa atgttaagtg gagaaatacg tatagctcca tataaacata
    10561 aagatggaac atcatgtgac ttttgtgatt actctgctat atgtcagttt gattcaacta
    10621 taaaagataa caagtataaa aatttaaata ataagagtaa tgaagagata ataaaaatga
    10681 tgaaaggaga tgttaattag tgagttctcc taaatggaca aaagagcaat tagaagttat
    10741 agaaagtaga gaatgcaatt tattggttgc tgctgctgct ggttcaggaa aaacagccgt
    10801 gctagttgag cgtataattc aaatgataac aagtagagaa aatccaattg atatagataa
    10861 gttattagtt gttacattta ctaatgctgc tgcgtcagaa atgagagaga ggatagggga
    10921 tgctatagga aaagccttag atgaaaaccc agaaaataaa catttacaaa accaacttgt
    10981 tttattaaat aaatctagta ttacaacaat acactcattc tgtttggatg ttataaaatc
    11041 aaactttcat agaataaact tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc
    11101 tattttgaaa caagaagcaa tagaggaagt atttgaagat ttatatgaag aaagagatga
    11161 ggggttttta aatttagttg aaagttatgc agaaagagga ggagataaag aagtacaaga
    11221 tattatcttg ggaatatatt catttgcaat ggcttctcct gaacctaaaa aatggttgat
    11281 tgactcagca gaacgattta atattgatga aaattttgat ttttcacagt ctatttgggc
    11341 tagagctata ttagacactg taaaaataga gattaatggt ctttgtctaa atatggaaag
    11401 ggcattaaaa gaagtggaat caatagaaga attagaaact tttgcagaaa agttatctgt
    11461 tgaatataaa aaaattgctg acatttcaca agcatgtaat aaatcttggg atgaggcata
    11521 taaaaagatg gcaagtatgt cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa
    11581 agatgctcct tcgtatataa aagaatcaaa agaaaaggca aaaactataa gagataagac
    11641 taagaagtct ctagaaagta tagtaagtgc cacattcaat aaagataatg attctattag
    11701 agaagaaata aaatatcttt ataacatagt taaaccaata tctagtgttg tactgagatt
    11761 tgaagaggaa tatagcaata agaaaagaga aaaaggaata atagatttta acgatataga
    11821 gcattttgca ttaaatatac ttacagatgt agatgaaaaa ggtaatattg taccttctga
    11881 tatagcagtt ggatatagaa acaagtttta tgaaatattt atagatgaat atcaggatag
    11941 taatctagtg caggaagtac tgcttaaagc tgttgcaaat acagaaactc caaatagatt
    12001 tatggttggt gatgtaaaac agagtatata tagatttaga caagcaaagc cagaattatt
    12061 tttgcaaaaa tataataact ataatgataa aaagggaagc tcccatagaa aaataatgct
    12121 ttacaaaaac tttagaagta gagaagaagt tgtagatgct gtaaattata tatttgaaaa
    12181 tataatgaat gagaatatag gagaaattga atatacagaa aaagaaagac taaatttagg
    12241 tgcaaatttt aatgtggata cagacgagaa atctattatt ggtggagcaa cggagataca
    12301 cctaatacaa aaagataata aacttgatga tgatattata aatgataaag acgatagaat
    12361 taataacaaa gaaaatgaaa tagaggaaga agaaaaatta gataatatac aacttgaggc
    12421 tagaatggtt ggaaacatta tcaaagattt aatgaaagtc aatgaagatg gaaaaattca
    12481 aaaggtctat gataagggaa tagatggata tagacctgtg gaatttagag atatagttat
    12541 tcttttaaga gctacatctg catgggctcc agtgtttgca gatgaattga tgaatatgga
    12601 tataccaacg tatgctgatg ttggagtggg gtattttgat acgattgaaa taaaaacaat
    12661 attgtcatta cttcaaataa tagataatcc aatgcaagat atacctctta tatctgtatt
    12721 aaaatcacct atatttggat ttacaccaga ggatttgatt gatattagag ttcagagtaa
    12781 agataaaatt ttttatgagg ttttaaaaag tacagcagag tatgatggat ttacagattc
    12841 tcaaaatgaa aatgagtctg agtttatacc aagtgaagag tgcataaaca aaagtaaaga
    12901 ttttttaatt aagctaaagg aatttaaaga aaagtcaatg tacatgagta cagatgaatt
    12961 tatatggtac ttgtacacaa ggactggata ctatgcctat gttggagcat tgccaggtgg
    13021 aagtcaaaga caagctaatt taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac
    13081 cagtcttaaa ggaatattca actttgtaaa ttttatagag aaattaaaaa aatctagttc
    13141 tgatatggga agtgcaaaaa cacttggtga gaatgcaaat gttgttagaa taatgagtat
    13201 tcataaaagt aaaggtctag aatttccagt agtcatatgc tctgcaatgg gtaaaaattt
    13261 caatactcaa gattttaaaa agagtatttt atatcatcat aatttagggt atggacctca
    13321 atttgtagac tatgagagaa ggatttcttt tccaagtata gccaaagaag cattaaaaag
    13381 caaaataaat atagaaaatt tatcagaaga gatgagagtt ttatatgttg catttacaag
    13441 agctaaagaa aaattgatta taactggttc tactagaaat atacaagata gtataaagag
    13501 atggtcaaat ggaattgaaa gtttagatac aatatcacaa tacgagatat taaaaggtaa
    13561 aaatttttta gattggataa tgccttgtgt attaagacat agggatttat ctaatctact
    13621 agaagaagta ggattagacg cagtttttaa tgttgaacat aattcaaaat ggtatggaaa
    13681 gttgtggaat aagaatgata ttttagtgga aaagaagagt gatgaagaaa aagaaagtat
    13741 tgaagaaatt cttgaaaaaa tagatgtaga taatcctgac agtgattatt acagtgaaat
    13801 agaagaaaaa ttaaactata tatatccata tgaatttagt acaagaaaac ctgctactat
    13861 atctgttaca gaaataaaaa agatacaaaa taattatgaa gaagagttaa taaatactat
    13921 atttgaacaa aaagtaatat taaaaaagcc tctatttatt caaaatgaag aagaaagaga
    13981 aaaaataagt ggaacagaaa gaggtacaat agtccattta gttatggagg ttttagactt
    14041 aaaaaatgta agtagtgtaa atgatataaa atcacaaata agaggttttg tttcaaaagg
    14101 tataataaca gaaaaacaag ctagtatagt aaatccatat aaaatatata aattttttgc
    14161 ctctaacata ggaaaaagaa tgttgaatgc tgaaattata aatagagaaa aatcaattta
    14221 tgcacaagta aatatgaaag acatatatat ttatgaaaag cttataaaca atgatgataa
    14281 aaaactttat gataatgaaa gtgtcatgtt aagaggtata gttgatgctt attttgaaga
    14341 agataatcaa atagttttgg tggattataa aacagatttt gtaaatgagg aaaatataaa
    14401 tcaaataata gagaaatata aaaaacagtt ggatttatat gcagatatta ttgaaacttt
    14461 aactggtaag tcagtaaaag agaaatgtat atatttattt ggagttgatg aagctgtttg
    14521 ttattagttt ttatatataa attaaaccta aaagatagag gaaggggaaa aaatgagatt
    14581 tattcataca tcagattggc atctagggaa gtccctagaa ggccattcta gaattgagga
    14641 acaagcaaag ttttgtgagg aatttataaa aatagtagaa tcaaatgaaa tagatatggt
    14701 tataattgct ggagatgtat atgatacttc taatccacca gcacaagcag aaaaactatt
    14761 ttatcagact gtatcaagac ttgccaataa tggtcagagg tgtgtattaa taatttcagg
    14821 aaatcatgat aatccagaga gactctctgc aataacacct ctagcacatg aacaggggat
    14881 acttatatat ggttatccat taagtgctac gatagaagct aagtataaag ggtttgaaat
    14941 aacatatgca actcagggat gtacaaaact gaatataaat ggagaaaata ttgtggttgc
    15001 tacattacct tatccaagtg aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt
    15061 tgagaaacaa aaaaattatt ctgaaaaggt aggagatata tttagaagtt tagaagaaaa
    15121 ttttagaagt gatactataa atatagccgt atcacatata tttgtaatag gtggagagag
    15181 tactgaatca gaaagaccta tacaattagg tggaagtttt ttagttgaaa gaaaagattt
    15241 gccagaaaag gctcaatata ctgcactagg tcatttacat aaacaacaaa aagcttccga
    15301 acgtttaaat gcatactatt ctggttctcc acttcaatat agcaaggatg aaagagcgta
    15361 taccaaaggg gcatacatag tggatataaa agctggtgaa aaaccaataa ttgaagatgt
    15421 atattttaat aattacaaac ctatagaagt atttaaatgt aatggaatag aagaagcact
    15481 agatatatgt gagcaaaatc aagatagaga gatttggtca tattttgaaa taaatactga
    15541 tgagataata tcacagaatg aaattaaaaa aatgaaggaa ctattaaaag acataataga
    15601 aataaaacca ataataacat cttgttatga acaagaaagt gtagatataa aagaaaagtc
    15661 tatggcagag ttgtttagag agttctattc ttttagtaaa ggtgtagagc caaaaggaga
    15721 acttatggat ttatttctag atataataag tgaagaaggt gaatcagcag atgagaccaa
    15781 ttagattaga acttacaggg cttaatagct atattgataa acaagttata gactttgaaa
    15841 aattaattga aagaggtttg tttgggatat ttggaactac tggaagtgga aaatctacaa
    15901 tattagatgc aataactatt gctatgtatg gaaatatatc cagaaataca aaagaatata
    15961 taaacagtgt gtgcgataag gcaattatat catatgagtt tgagatagga agcaaaaaca
    16021 ctagaagaag atacattgta gatagaacca ttgctagaag caaaacagga acaaagacct
    16081 ctaatgctag attaatagaa gtattgaatg acaataccca aaatgtttta gctgataaag
    16141 ttgtggaagt aaatgaaaaa gtagcgcagg tcgtaggatt aacggcaaat gattttacta
    16201 ggtcagtagt tttaccacaa gacaaattta atgagtttct aagattatca cgtgcagata
    16261 gaagagacat gttagagaga atttttaatc ttgagaaata tggaagaagt cttggagaaa
    16321 aagtgaaaaa gagaaaaaat atacagttac aaaatttaaa agatttgaaa tcaaaattaa
    16381 gtcaatatga tggaattaca gaggaagtat ataacaatat aaaccaagaa ttaatagagt
    16441 taaaaaattt ggaaaaagat aagaataatg ctttagacct agctcaaaaa tcttatgaag
    16501 aaagtaaaac agtatatgaa gaacaattaa aattagaaaa aaatgaactt agaaaaaatg
    16561 agcttgattt aaaaaatagt gagataaaag aaaagattgc tcttgtagaa aatgatagta
    16621 acgctaaaaa ggtagaccca tacataagtt cagttcaaaa tctagaaaaa aagatagatg
    16681 aagatagctt tgcagtaagt aatttagaaa agaaattagc aatattaaat caggagcttg
    16741 aagttacaaa aaatagatat gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat
    16801 tgagtgaaga aaaaataaga ttgcaacaag caataaaatt agaagaagag ttagttttat
    16861 tagatagaga gttaaaagag ctaaaagaaa gtggaattaa tttaaataag actaaagttg
    16921 aattagaaaa agtaaagcag gtgtcagagt ccagaaaaga tgcagttact aaatctataa
    16981 aagaagttga aggaaagatt gataaagtaa atataagtgc agaattaaaa caaaaaatat
    17041 ttctagcata tgaatacgag aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc
    17101 taaataaact agaagaaata ctcaaagaga cagaaaatat aaatttaaaa gttagatata
    17161 ttgataaaga taagaatgat gtaaatcgta atttagaaaa tttatcactt cacttggatg
    17221 ttcttcttaa aaaatgtcct ggtaagagtg ctgatttact tttaaaatct gaatatgtta
    17281 ctgaattaag aaataaagca aataatacta aagaaaatga aattaagaaa agcagtatac
    17341 aagatgaatt aaagataata cttgaaagta agtttaatac tgaaagggaa gtaaatttac
    17401 taaatgaaaa acttgaaaat aatagaaaga acagagatga gctagaaaaa gaattagaag
    17461 aactaaagta tttaaattta gcctctgaac ttagacgtga attaaaagaa aatatgcctt
    17521 gtccagtttg tgggtcaaaa catcatgaaa atcataatat aactaaatat gatgaaaata
    17581 tatcttttgt caaagaaaaa ctggagaaat tagaaaaaga aaaaataagt ataagaaaca
    17641 atatagagga attaaatgct aaagtaagtg ggtatttatc tatagaaaaa atgaagacta
    17701 aagaacttga agatgtaaaa ggaaaactag gggggattcc atctagtcaa ttattaaaaa
    17761 aacttgatga agaacaaaga aaacttgcat tgttaaaatc taatattcag gagtgggaga
    17821 aagaaaaaga gagtactgaa aataaagtaa cattagctaa agaagaaaaa aataaaattg
    17881 aaaaagaaga attaaaaata agagagagtc tgaataatta taagaaatta acaagagatt
    17941 taaacataga aatagaaggt ttagaaaata aatgtaaaaa acttaaacag gaatacctag
    18001 gattaaagac gattactaaa gtctctaatc tattgtcaaa agtaaaagaa ataagagaga
    18061 atgaaaaaga attagaatta ctaagtactg ggcattcaaa tttattaaaa aatagagact
    18121 cactagatat taaaataaga gaaaatgaga gtaaactaca tgaaatagag atagaattaa
    18181 taaaggctag agaattatat gtagaaaaga aattgagtag ggataataag tataaagaag
    18241 ttattagcat aacgaaaggt gatttagcta aaaatctttt acataatgtt gaagaaaata
    18301 tatgtaagat attagaacaa gaagaatcaa gtaagaaaaa attggaagag caaagattag
    18361 aatatgaaaa aaacattgct gaaaagcaca atatagatgg tagacttaaa actgctaagg
    18421 aacaatataa agaccaaaaa tcaacactaa ataaactatt ggcagaaaat aaatttgaaa
    18481 gtatatatgc agtaaaaaga gctttattag atactgatgc agtaaaaaaa ttgattgaag
    18541 aaatactaga gcatgaagaa gaacaaaagt tattatcatt taaaataaaa tcatcaaaag
    18601 aaaaattaaa tggaagaagc ataaagaagg agtactttga acagttaaaa gatgaaattt
    18661 ataatttgaa agtagaaata ggaaatatat ccaaagatat tggagctaat caaaatcagt
    18721 taataacatt aaaagactct ttagataaga taaatgattt taataaacaa cttaaggtag
    18781 ttgaacacaa tgtggactta ttggaagaat tagataaatg tgtacaagga aataaatttg
    18841 tagaatatgt ttctacaaac caattaaaat atatagcgtt ggaagcatca aaacgattgg
    18901 atggaataac aaaaggaaga tatgcacttg aaattgactc aacactaaac tttgtaatga
    18961 gagataactt taatggagga gaaagaagaa gcattgatac tctatctggt ggagagacat
    19021 tcttgacatc tctatcatta gcacttgctc tttcatctca aatacaatta aagggaagtg
    19081 caccattaga atttttcttt ttagatgaag gttttggttc attagatagt gagcttttag
    19141 aaattgttat agaatcattg gaaagattgc atagtaataa tctaagtgtg ggtataataa
    19201 gtcatgtcga ggaacttaaa aatagagttc ctgttaagct attagtttca tctagtgagg
    19261 ctggaatagg ttcaaaagta aaaattgaat atagttagaa aatatatgtt aattgaatat
    19321 attatctaat atttccaaat aatatgtaat tgtgatataa taaaaactaa tattaaagtt
    19381 atttataatt aatattaata taaaaacatg taagtaggag tgtggtaagt gttggaatcg
    19441 cccaataatt tgattcagat tattttttta atagtattac ttatagggtc tgcatttttc
    19501 tcagcatctg aaacggcctt aatgtcgttg agtaaaatca gaatcagata tatgcaagat
    19561 gaaggagtta aaggagctaa gttagtaagt tcattaatag aaaatccaaa taagctttta
    19621 agttctatat tggttggaaa taacgtcgta aatatagcag caacatccat atcaacatct
    19681 ttgtttatag gattaatggg agaaaaagga gtggcacttg ctacagctgt tatgacagta
    19741 ttagtattaa tatttggtga gattactcca aaaacaatag cagcaaataa ttcagaaaag
    19801 gtttctctat tagtatctaa acctataaaa gcaattatat ttatattaag accaatagta
    19861 tggatattta atataattac aaatattata tttaaattat ttggtataac aaataaagga
    19921 gctaaatcat tcataactga agaagagcta aaaactatgg taaatgtaag tcatgaagaa
    19981 ggcgtccttg aaatggaaga gagagaaatc ataaataatg tttttgaatt tggagatatg
    20041 caagctaaaa atgcaatggt acaaagaata gatatggtag ctatagatat ggaagatagt
    20101 tatgatgaga taatacaggt atttaaaact gaaaaattaa gtagaatgcc tgtgtatgaa
    20161 gaaactatag atgatattgt aggaatactt aatataaaag atattatatt tttatctgat
    20221 gaagaaatag agtcatttga tattaaaaat tatatgagag aacctttttt cacgtatgag
    20281 ttcaaaaaga taacacaact tcttgaagag atgaaacttg aaaaaagtca gatggctata
    20341 gttgtagatg agtatggtgg aacatctgga cttttaacta tagaagattt agtagaagtc
    20401 attgttggag atatagagga tgaatatgat gaagaagagg atgaaataca agttataaaa
    20461 gaagatgagt atattgtaga tggaagtact aaaattggtg atgttaatga acttattggt
    20521 gtaaacttgg aatctgagga gtttgattcc ataggaggtt ttataattgg acacctaagc
    20581 agattgccag aagaaaatga agtaattgaa gttgataata taagattttg tatagaaagt
    20641 atagaaaaaa atagaattaa aaaaataaga atatatacat aatgtaataa tgttcttaaa
    20701 tttattagaa gtgtataaat ataaaagata tcttttatat ttatacactt ctttttattt
    20761 tgtattcaaa taaaactagt atgaaatgga caaaataaaa atataaatta gaagaaagga
    20821 gatttttaag atgagcatac tagaaaataa ttttttatac aaattaaaaa acaacactat
    20881 ctttttttgt atatatacat tgttatttat ttttatttac aaagcgtttc catacatagc
    20941 tccatttttt ctaggtgcta taatagcact tatgattaat cctatatcac aaaagttaga
    21001 aaataagttt catataaata aaggtatctc tactcttgtt ctaagttttc ttgcagtggc
    21061 tatagtcagt acagttacta caataatagt aataaattca atgaaagaat taatgggatt
    21121 tttaaataat atatctgcta atcctgagga tataagtaat actattatgt attttttaaa
    21181 taaaataaat gactttatga aatcatttca agaaatagcc aattttgatg tagaacaatt
    21241 agtaaacaaa ttcagtggtg aagtaatgca aattacaaaa aatcttctta ctagtatact
    21301 tggattggct acttctattc catatattat aatatttata attaccttgt ttattgctac
    21361 gtactttata gcaaaagacc ttgataagat tgaaaatagt ttttataata tgtttactgt
    21421 tgatgttaga aagaaggtaa aaaacgtaaa gaaagaggca gggctttcac ttgttggata
    21481 tataagggcc tatacaatac ttatggctat aacatttttt gcaatctggg gaagttttgc
    21541 tttatttgga ttgaaatacg gtctaatagt agggtttgta ggagctttaa tggatttaat
    21601 tccattccta ggtataataa caatatatct tccagtaatt gtttattatt ttcttataaa
    21661 aaattatttt atagctataa gtatgactgt gatatttttt gttttgtctt tgattagaga
    21721 aatacttgaa cctaaactag tatcagtaaa tgttggacta aatccattag ctaccctagc
    21781 agcgattttt attggaatcc aagtaaaagg aataattgga gtaatttttt gtttgggttt
    21841 ggtatgtatg catgatatac ttaagaaggt ggatattttt taattataat aattgataga
    21901 tttttttaaa aataaaattt tattcacagg taaataaaaa aatattaatc ttattaagaa
    21961 agagggtata tacgtgaaac aaaaagtagt ggaaagattt ttaaaatatg tatcatttga
    22021 tacaacatct aattctcagt gtgagaactg tccatcttca gaaggtcaaa gagttttagc
    22081 taagtatata gttgaagagc taaaaacaat gggtgttgat gatgtgagtt tagatgaaaa
    22141 tagttatata atggctactt taaaaggaaa tactgatggt atggatacta taggattcat
    22201 atcacattta gatacaattg aagatgttag tggaaaagat attaaaccaa gaataataga
    22261 aaattatgac ggaaaagaca tagttttaaa tgaagcgtta aatgttatta catatgtaaa
    22321 ggattctcca gagttggaag aatttaaagg tgacgattta atagtcactg atggaacaac
    22381 tctacttggc tctgatgata aagctggtat agctgagata gtgactgcaa tagagtatct
    22441 aataaatcat ccagaaatta agcatggaga tataaaaata ggatttacac cagatgaaga
    22501 aattggtaga ggtgctgatt tatttgatgt tgaaaaattt ggagcaaaat atgcatatac
    22561 tttagatgga ggaatagttg gagaacttga atgtgaaaat tttaatgctg caaatgcaac
    22621 cattactata catggtagaa atgttcatcc aggttcagct aaaaataaaa tggtaaatgc
    22681 tatacatata gctgctgaaa tatcagaaat gtttccagca gatgaaagac cagaaacaac
    22741 agaaggatat gaaggatttt ggcatttaaa ttctataggt ggaaatgtcg agaatgtaag
    22801 tatggcttat attattagag accattgtag agaaaaattt gaaaatagaa aatctattat
    22861 gatagagaat atagaaaaaa taaataagaa atacgataat agagtggaat tggatttaaa
    22921 agactcttat tacaatatga aagaaaagat tgagccagtt atgtttatag ttgatatagc
    22981 taaagaagct atggaggaat taggtataaa accacgttta gttcctgtaa gaggtggtac
    23041 tgatggagca agattatctt ttaatggatt accttgtcca aatatattta ctggtggatt
    23101 aaacttccat gggaaaaatg agtgtatacc agttagttct atggagaaag ctactaaact
    23161 aattgtaaga atagctgaaa aatatgctga aagagtttag ttaaaaagta tttaaaaatt
    23221 aagcatttaa agttataata gctatatcat tctttataag tcaagctgat tataagcttg
    23281 tgttgtactt aaaatggtat agctattttt gtaattattt ttataaaaga taattatttt
    23341 tagtttaggg tatcaataaa aagtatgaat atcaataggt attaacattt aaaaggggga
    23401 attgtattga aaattaaatc taaaaaaata atagcatttg cactttcact tgggattatg
    23461 ttgccaagtt gtgtatatgc agacactatt agtaaaaatc aaaatgcaca ggtagatatg
    23521 aacaattatc ttcaaggtag aaaatctcca aaggaggaaa aacttattgg tgaagataga
    23581 tttgatacag cgataaaaat atctcaaagt ggttggaata atggttcaga aagggtgttt
    23641 ctagttaact caaattcatt gccagatgca ttggcttcaa ctcctttggc aagtaaattg
    23701 gatgcaccaa tactgcttac aaataaaaat agtataccat ataatgtaat agaggaaata
    23761 aaaaggctta atccagcaga agttatttta attggaagtg aaggagctat ttcatcaagt
    23821 gtaaaagaat ctcttgaaga tatggaagta ttagtcagta gaatcggtgg agcagataga
    23881 gaagaaacat ctttacttct tactagacag ctagatgata ctggtgattt aggtgtctca
    23941 aaagtggcag tggttaatgg gtataatgga ctggcagatg caacttcaat atcaagccca
    24001 gctgcaagtg ataatacagc tataatatat acaggtaagg atagcataag aagtgaggca
    24061 aagaatttta ttacacaaaa ctcaactaag acatacatag taggtgggga gtacagtata
    24121 agtaaaaaac ttgaaggaca gttagtaaac tcagaaagac tagcagggac agatagaaaa
    24181 gatactaatg cgaaagtttt agaaaagttt tatggaaaat caagtaaggt taataatatg
    24241 tattttgcaa aagatggaag tggtagagaa gctgatttag ttgatgggct tgctgctgga
    24301 gtattagcat caaaaacaaa gtcacctgta gtacttgcaa gtggaagttt aagtagtgca
    24361 cagaaaagtt ttataaaaaa agtaaaggct gataaatttg tacaagttgg tggaggtaaa
    24421 aatagtaaac catatgcaga agcattagcc ttacagtaat attgtgtaaa tcaaaattaa
    24481 tatataataa tagcaagtaa accaagttgc acttcaatat atataataaa ataagtaaac
    24541 taacctatgt ttaaata
//
LOCUS       DAFKMX010000040        24466 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFKMX010000040 DAFKMX010000000
VERSION     DAFKMX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.040"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..804
                     /locus_tag="LZB08_001308"
     CDS             <1..804
                     /locus_tag="LZB08_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7314818.1"
                     /translation="NIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFK
                     LAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKD
                     GLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDI
                     IRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIY
                     SKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
     gene            812..2812
                     /locus_tag="LZB08_001309"
     CDS             812..2812
                     /locus_tag="LZB08_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7314819.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3074..4333
                     /locus_tag="LZB08_001310"
     CDS             3074..4333
                     /locus_tag="LZB08_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7314820.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKGKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4394..6181
                     /gene="iorA"
                     /locus_tag="LZB08_001311"
     CDS             4394..6181
                     /gene="iorA"
                     /locus_tag="LZB08_001311"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBM7314821.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6183..6758
                     /locus_tag="LZB08_001312"
     CDS             6183..6758
                     /locus_tag="LZB08_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBM7314822.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            6811..7881
                     /gene="buk"
                     /locus_tag="LZB08_001313"
     CDS             6811..7881
                     /gene="buk"
                     /locus_tag="LZB08_001313"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBM7314823.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8099..8800
                     /locus_tag="LZB08_001314"
     CDS             8099..8800
                     /locus_tag="LZB08_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314824.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            8860..9366
                     /locus_tag="LZB08_001315"
     CDS             8860..9366
                     /locus_tag="LZB08_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBM7314825.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9541..10563
                     /locus_tag="LZB08_001316"
     CDS             9541..10563
                     /locus_tag="LZB08_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBM7314826.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(10710..11018)
                     /locus_tag="LZB08_001317"
     CDS             complement(10710..11018)
                     /locus_tag="LZB08_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBM7314827.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11078..11506)
                     /locus_tag="LZB08_001318"
     CDS             complement(11078..11506)
                     /locus_tag="LZB08_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314828.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(11729..13186)
                     /locus_tag="LZB08_001319"
     CDS             complement(11729..13186)
                     /locus_tag="LZB08_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBM7314829.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13604..14518
                     /gene="nadA"
                     /locus_tag="LZB08_001320"
     CDS             13604..14518
                     /gene="nadA"
                     /locus_tag="LZB08_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBM7314830.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            14531..15832
                     /locus_tag="LZB08_001321"
     CDS             14531..15832
                     /locus_tag="LZB08_001321"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBM7314831.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            15813..16649
                     /gene="nadC"
                     /locus_tag="LZB08_001322"
     CDS             15813..16649
                     /gene="nadC"
                     /locus_tag="LZB08_001322"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBM7314832.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            16905..17237
                     /locus_tag="LZB08_001323"
     CDS             16905..17237
                     /locus_tag="LZB08_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBM7314833.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            17399..17584
                     /locus_tag="LZB08_001324"
     CDS             17399..17584
                     /locus_tag="LZB08_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314834.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            17611..18696
                     /locus_tag="LZB08_001325"
     CDS             17611..18696
                     /locus_tag="LZB08_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBM7314835.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            18689..19147
                     /locus_tag="LZB08_001326"
     CDS             18689..19147
                     /locus_tag="LZB08_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314836.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            19182..20273
                     /locus_tag="LZB08_001327"
     CDS             19182..20273
                     /locus_tag="LZB08_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7314837.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            20278..20502
                     /locus_tag="LZB08_001328"
     CDS             20278..20502
                     /locus_tag="LZB08_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314838.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            20512..21075
                     /locus_tag="LZB08_001329"
     CDS             20512..21075
                     /locus_tag="LZB08_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314839.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            21036..21665
                     /locus_tag="LZB08_001330"
     CDS             21036..21665
                     /locus_tag="LZB08_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7314840.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            21842..22594
                     /locus_tag="LZB08_001331"
     CDS             21842..22594
                     /locus_tag="LZB08_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314841.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISIDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            22607..23092
                     /locus_tag="LZB08_001332"
     CDS             22607..23092
                     /locus_tag="LZB08_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314842.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            23099..23248
                     /locus_tag="LZB08_001333"
     CDS             23099..23248
                     /locus_tag="LZB08_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314843.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(23384..24205)
                     /gene="yidA"
                     /locus_tag="LZB08_001334"
     CDS             complement(23384..24205)
                     /gene="yidA"
                     /locus_tag="LZB08_001334"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBM7314844.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
BASE COUNT         9495 a         2653 c         4417 g         7901 t
ORIGIN      
        1 aatatagatg aaggtataag ttgttatata aaaggtttaa atttagttaa aaagagttca
       61 ttgaatctta gaaaaatatc tatattttat gggctaatag caaatggata tgagctaaaa
      121 aaagatttta aactggcaat agaatattat aaaagtatgg attggtattc tgataggtgg
      181 gaaaaaaata attcctataa aacatctcaa gcaattcaca aacaatatga actcagacaa
      241 aaacaagaga gtttaaggtt actagtggat gataataaga aattagaaaa tgatatttta
      301 aaagatggac ttactaaact atataataga agatatctgg aacagcagtt ggatttattt
      361 aatggaacca gagacaaaaa tatggtcgca atactggtcg atatagattg ttttaaaagt
      421 tataatgata attatggtca tctagcagga gataaagtta tagttaaggt aactgatatt
      481 ataagaagtg tatttataga tattaccgaa cacataatca gatatggggg agaagaaatt
      541 ttaatattag tagattctat agaatcaatc ggtgttgaag agattgataa tatagaatta
      601 tatattaaaa gagttttcaa acttcttgaa tttgagggta ttgaacatat atattctaaa
      661 ataaaaaact atataactat aagtgctgga gtaagtataa aaagatgtcg tagtagaaaa
      721 gatgttgaaa tattaattga agatgcagat aagaacctat ataaatcaaa aaaagcaggt
      781 agaaatcaat acattataat ttaaaattga ggtgattttg ttgcgaaaaa aaataggaat
      841 tatagcctct gatattgaat taaaagaaag aatagaagaa ttatatagag aagatgtaga
      901 aaatggaact ataataatcg acatacttaa cctagactta atggaaaatc aaggtcgtat
      961 acttgttgaa aaaggtgccc aagctataat tggtagaggt ggaggttata gtttagtaat
     1021 agatacagta aatgtacctg ttataccaat gaatatgaag tcaactgacc ttttaagagc
     1081 aatagaaata gcaaagaaat attctaaaaa agtagtattg attcttggag acaatgaagt
     1141 ttcttttgac tatgttggct ggagaaatgt cataagtact gaaataactg aagaatggtt
     1201 tgaatctaag tatgagataa gaagtaaagt tgtaaaatat atagaccaaa aagatgaagt
     1261 tgtaatcgta ggaggaggtt tggcatgttc ttttgcaagg caatatggaa ttgatagtgt
     1321 atttgcaact gctagtgatg aatctataag agaagctgta gaatattgta aaaaattatt
     1381 agatacatta ggtgaagaaa agtttaataa tgaagtgtta cgaaatatac ttgatggtat
     1441 aaaagatgga gtaattgcta tagatagtaa tggcagtata attttgtaca atgaaagtgc
     1501 aaaaaacatg cttaaagtag agagaaaatg tgcacttaat aaatacatct tagatgtatt
     1561 tcctaaaatg gagtggatgt tagattgttt acatgaaaaa gaggacgtag aagatagaaa
     1621 gataagaaat ataaataatc taattgttaa tactaggaca acattgataa aagttgataa
     1681 tagtacttat ggagttttag gaataataca agatataacc aagctacaaa acttagaaag
     1741 aaaaataaga tttgacttga atcaaaaagg tctctatgct agatatactt ttgatgattt
     1801 tttatttaaa gataaattaa ctaaagaatt tattgaagaa gctaaaaaaa ttggaaaaag
     1861 tgattataca actttacttt atggagaaag tgggtctggt aaagaaatta tagctcatag
     1921 tatacacaac ataagtaaaa gaaaagatag gccttttgtt gctataaatt gtgctactat
     1981 agcagaaaat ttacttgaaa gtgaactttt tggctatgaa gaaggtgcat ttactggtgc
     2041 tagaaagggt ggaaaaagag gactatttga acttgctcat ggagggactc tttttttaga
     2101 tgaaataaat agtctatcct ttaatattca aacaaaacta ttaagagtaa tagaagaacg
     2161 tcaaataatg agaattggtt ctgattatat aatacctctg gatataagga ttatagcagc
     2221 aacgaatgaa tctttaaccg aaaaaatagt tatgggtaca tttagagcag acttatttta
     2281 tagattgagt agtttagaga taaatatacc tcctttaaga gacagaagag aagatataat
     2341 acctttgttt aacaattttg taaatgaagt attaaaggat gatggtctca atggaataaa
     2401 cagtatagat gaaaattttg ttttgactaa agatgaaatg gataagctat ataattatag
     2461 ctggcctgga aatgtaagag agttaaagac aatagctcaa aaatatgttg tgacaggtaa
     2521 aattaaattg agacaagata gagattttaa aactaaaaaa ttacttccta actctgaagt
     2581 agacaaattt aattctgaaa cgacagcaag tgtagaagtc caagatgaat ctataaatat
     2641 aagtaagata aatgatggta agataagtat agatataaaa gaagtaaata agtatgttga
     2701 agaaaaaata ataagtatgc tttttgctca gggactttct aaaaatgaag ttgcacaagt
     2761 tcttggaata agcaggacat ctttatggaa gaaatacaat aaaaatattt aaatataatt
     2821 acatataaat tattttgaag taatgttaat tttttaaaac aagtgttaag aaaaattaac
     2881 attacttttt tgcaaatttt tattttttta gacaggcata tatatttata agaaaaatag
     2941 ttaaaacact taatttaata atgaaaaaaa taataattct aaacaaaatc aattttacat
     3001 aaagtgggat ttattggcat agtaattgct taatatataa tgtaagagta aatacaattt
     3061 taggggtgat ggtatgagtc aaagtatggt tggtaagcat gcaatgtggc caaaagaaaa
     3121 tgatgttata tttagcatat ctggcagagc tcaagcagca gagaaggctt ttggtatgga
     3181 taatgtaata aatgcaacaa taggcgctct aatggatgat tcaggaaaat tgataacaat
     3241 gaaaactgta tatgaagaat ataaagcatt agataactgt gagataggtg catatgcagc
     3301 tcttgaaggt caaccagatt acttagaagc agttaaaaaa gtatttttca gagattactt
     3361 accagaagga catataagag tattagcttc accaggagga agtggtgcaa taaaattagc
     3421 tgtatggaac tatactaatg aaggagatga ggtattaaca tctgattggt tctggagtcc
     3481 atatgtaagt atagctgaag aggctaatag aaaagtagtt aattaccaac tttttgatga
     3541 aaatagaagg tttaattttg agtcgtttaa agaaaaattt gtaaatatag cagaaaaaca
     3601 aggtagagtc tttacaatta taaacacacc agcgcataat ccaactggat atagtgttgc
     3661 agatgatgag tgggataaaa tattagactt atcaaaagaa gtcgcaaaag ataaaggcaa
     3721 aaagataata tttttcgtag attcagcata tattgacttt gcaggtgatg atgatgtatg
     3781 tagaaagttt tttaagaaat tttctaattt gcctgaaaac gttctcgtat tggttggatt
     3841 cagtatgtca aaaggattta cagcttatgg tatgagaatg ggggctataa tttgtatatc
     3901 ttcaagtgaa gatgtagctg aagagttcca ttactcatgt gttcattcat gtagagcaaa
     3961 ttggtcaaat tgtaatagaa gtgccatggc agtattatct aacattgtaa atgaccctaa
     4021 aaaattcaaa gaatatgagg atgaaaaaga aatatacaaa aacatgctta ctagaagagc
     4081 agatgtattt gtaaaagaag ctgaaagagt aggattagaa attcttccat atatagcagg
     4141 tttctttgta agtataccat gtgataatcc aaaagaagta tgtgaagaat tgactaaaca
     4201 taatttattt gcagttccat taaaaatggg cttaagattt gcagtttgtg cagtttctga
     4261 agataaatgt aagaaagcac caagtattat aaaagaagca cttgaaagtt tagaagtaaa
     4321 aataaataat taatttcatt gaacaaattg ggtggactat gaatttaata attgtagatg
     4381 gagggaattt tatatgaaac aattgatgac aggtaatgaa gcaattgcac gtggagccta
     4441 tgaggctggt gttaaatatg catctgcata tccagggact ccaagtacag aaatattaga
     4501 aaatatagca acatataaag atgcaatagt tgcagagtgg gctccaaacg aaaaggtagc
     4561 acttgaggct gctatagggg gttcaattgc aggagcaaga acaatggctt ctatgaaaca
     4621 tgttggtttg aatgttgcag ctgatccaat atttacttat gcatatacag gggtaaatgg
     4681 tggtatggta cttattacag cagatgagcc gggaatgcat tcatctcaaa atgagcaaga
     4741 caataggatg tatgcaaaat ttgcaaagat acctttattt gaaccatcaa ctagtcaaga
     4801 agctaaagat atgataaaag aagcttttga agttagtgaa aaatacgata caccagtttt
     4861 atatagagtt acaacaagac tttgtcattc aaaaggatta gtggagtgct atgatagaga
     4921 agaagttgaa attaaagagt atgttaaaaa tgctaagaaa atggtaactg ttccagcaaa
     4981 tgctcaaata agaaggggcg ttgttgaaga aagaatggaa atccttaaaa aattctcaaa
     5041 tgaaactgat ttaaactact atgaaataaa tgatacaaaa ataggtgtta tagcctctgg
     5101 tatgtgttgt aattttgcca aagaagtttt tggaaaaaat gcatcatata tgaaactagg
     5161 atttacaaat ccattacctt atgaaaaaat aaaagaattt gcagaaaaag ttgataaaat
     5221 atatgttata gaagaaaatg acccatttat agaagaacaa ataaaagcat atggaataga
     5281 ttgtattgga aaagatgtaa taccaccata tggagaaatg acaccagatg ttttaagaaa
     5341 agctatattt ggaaaaacaa atgatactat agaatacaaa tctgaattag taacaccaag
     5401 accaccaagt ttctgtgcag gatgtccaca tagaggattc ttctatgaac ttggaaagag
     5461 aaaaaattta atagttggtg gagatatagg ttgttatact cttggatttg ctcctccata
     5521 taatggaata gactatgttg tttgtatggg ttctgctttt ggaactgcac atggagctca
     5581 aaaagttctt aatatgaagg atgataatga aaaaagattg gtaggtgtac ttggagactc
     5641 aacattcttc catacaggaa taaatggact tttagatgtt gtttacaata gaggtaattc
     5701 tatttcggtt atattagata atagaataac tggaatgaca ggtcaccaag aaaacccagg
     5761 ttcaggatat actttacaag gtgctaaaac taaagaagtt gatatagaag gattagttaa
     5821 agcttgtgga atagaacatg taagagttat aaaccctaac aatttaaaag aagtaaatga
     5881 agcactagat tgggctttag ctatagaaga tgaaccatca gtaataataa caagatggcc
     5941 atgtgtactt aaaaaattct ctaaagaaga tatagaagaa tttaataatc catttaagac
     6001 taagtgtaaa gttgaccatg ataaatgtat aggatgtaaa ttatgtttga aaactggatg
     6061 tccagctcta tcatttgata aagagaataa gttatcaaat atagatagaa atcagtgtgt
     6121 aggatgtgga gtatgtgcac aagtttgtcc aaaacaagca atagttaagg aggaaaaata
     6181 atatgactaa gagtatactt ttggtaggtg taggaggtca aggaactata cttgctagta
     6241 aattattaac tatgggattg atggaagctg gatatgatgt aaagatgagt gaaatacatg
     6301 gaatgagtca aagaggaggt tcagtttcct cacaagttag atatggagat tgtgttcatt
     6361 ctccagttat agaaattggt ggagcagata tattagtatc ttttgaaaaa atggaagctt
     6421 taagatggtt caattattta aaaccagaag gaaaagcttt agtaaataat cataggatag
     6481 attctatgac tgttttaatc ggaggagctg aatatcaaga aagtgatata gatgcagagt
     6541 taagtagatt aaatgcaaaa gttataaatg cagctgataa agctgaagaa ctaggaaatt
     6601 caaaaataat gaatgttatt ttattaggat gtctagttaa atctatgcaa ttagagtcta
     6661 ttgattggga aaaaattata agtgaaaatg ttaaaccaaa atttgttgaa ttaaacataa
     6721 aagctttcca tgaaggtatg gaaatggtta gcaaatagtt tataatagat ataaatagac
     6781 tatagaaatt aaggtgaaag gaagaaaatt atgacttaca gaatattagc cataaatcca
     6841 ggttctactt ctacaaaaat agcagtatat gatggagaag aacaaattct tgtgaagacg
     6901 atagaccatc cggctgaaga gattgcaaaa tataatacta tacaagacca gtttgaaatg
     6961 cgtaaggaag cagttttgaa tattcttaaa gaaaatagta tagacttaaa atctcttagt
     7021 gcaatagtag gaagaggtgg agttttacca ccagtaaaat caggagcata tttagtaaat
     7081 gaagaaatga ttgatgtact aagacataga ccagtacttg aacacgcttc caatttaggt
     7141 gctgttgtgg cacatgcaat atcagaacct cttggaatca actcatatat ttatgattct
     7201 gttgcagtag atgagcttat agatgtagcg agaatatctg gactttgtgg aatggataga
     7261 tcaagtgcag ggcatgcatt aaatactaga gcaatggctt taaaatatgc taaggataaa
     7321 ggaaaagatt ataagagctt aaacttaata gtagctcata ttggtggagg agtaagtatt
     7381 tatcttcatg aaaaaggaag aatggttgat atgctatctg atgatgaagg accattttct
     7441 ccagaaaggt caggaagagt acctgctaca aaattagtgg ctgcctgtta ttcaggtcaa
     7501 tattcagaaa gagaaatgac taaaaagata agaggtaaag gtggtatagt ttcataccta
     7561 aatactgtag atgctagaga agttgaaaaa atgatagcag aaggaaatga agaagcaaaa
     7621 attatttatg aagcaatggc ttatcagtta gcaaaaggta ttggagagtt agcaactgta
     7681 gtagatggaa aggtagatgc tataattata acaggtggaa ttgcatattc tgaaatgttt
     7741 acttcaatgg ttaaaaagaa agttgagttt atagcaccag tagaaattat ggcaggagaa
     7801 aatgagttgg aatcacttgc ttttggaact ttaagagtac taaatggaga agaagaagct
     7861 agaatttata gtgaaaatta ataagaaaaa ctaaaaatgt ttatatacta aaaaagtggt
     7921 atatccgttt tttatacacg aaagcttaga gtataggtga aaatgtataa aatttggatt
     7981 gccacttttt ataattaaaa ttttatctta acctaataaa taattctgta aaatttcgta
     8041 tttatatata tattttattg gtatttattg ttattattat tatgaggagt gatttttgat
     8101 gggaaaaata agtttaaaaa gtatgataag attattttca ggttttttta tatttgcaat
     8161 ttcttctgtg cttatgatta atgcacatgt aggtcttatg ccttgggatg tacttcacca
     8221 aggtttatct ataaagctag gcataactat aggtcaggct tctataatgg taggagttgt
     8281 aattgttata ttggatgcag tttttggtga aaatatagga tggggtactt tactcaacat
     8341 gacatttata ggaattttta ttgatttagt aatattttct ggagtgatac ctcatgcaag
     8401 caacacttat ataggtgtgt ttatggttgt tataggaatt attttggcag ctatagcaag
     8461 ttttctatac ttaggtgttt gtttaggaag tggaccgaga gatgggctta tgattgctct
     8521 tcagaaaaaa actaataaat ctgtacgtct tgttagaaca attcttgaaa tactagcatt
     8581 agttgttggg tggttattag gaggttctgt aggtattggt acgcttgtaa gtgctttagg
     8641 tttaggatat gtattacaaa tagtgtttag aatatttaaa tttgacacaa aattgttgaa
     8701 acatagattt attatagatg atataagaga atggaaggaa aagaaaagca atgaacataa
     8761 atgtaaatca agtattgtaa taaaaaatga gcagaattaa ttatgctaaa atatatttgt
     8821 agtttatcta atttatagta aagtcaagga gattttgaaa tggaattatg ggatttatat
     8881 aatgctgatg gaattaaaac aggaaatgtt ataaaaagag gtaattctat tgaagaaggt
     8941 tattaccatt tggcagttga agtgtggatt ttaaatagta actctcaaat tttaattcag
     9001 aaaaggtcta aaagtaaaaa gacattgcca aatatgtggg gaatgacaac tggatgtatt
     9061 gtttctgggg aagacagttt agaaggagca attcgcgaag caaaggaaga aatagggata
     9121 gatataacta aagatgagat gaaagttttt agaagtatga ttcatgaaga tactctttgg
     9181 gatgtatact tagttaaaaa agaatatgat atatccaaag ctattttaca ggaggaagaa
     9241 gtaagtgata taaagtgggt aagcacagat gaaataagac aattactaaa agagggtttg
     9301 ttttttgaat atccagagat atacgaattg ctatatgaga tagataataa taaattaaat
     9361 atatgatgta gagcagattt cagtataagt aagattattt aaaatgtatc ttctctaaat
     9421 tttaatattg ttttatgaaa attaataagt tatttttagt atttttctac ttgttttttg
     9481 agattaaaat aaaaagggca gtcattataa agtcataaaa atttggggag gatttaaact
     9541 atggaagata acaaaggatt ggcacgtgac agttcaaaat ataaaattgt ttctatatct
     9601 gtcgtagtaa ttattctaat tacattttgg tatatgttaa atatggtgct tttaacattt
     9661 attatgactt ttattttcta taacttgctt gtagccacaa gaaaacgaat taagaagttt
     9721 tcttctttaa atattccaga ttcattgata attatagtgt tatatgcact atttgctata
     9781 ttgttagtat tgattagcta tgcagttgta ccaataatta tagttcaact tacagagttg
     9841 agtagggttt ttagtgattt tgatgtaaat caatttgctc aatctttagg accaaaattg
     9901 tatcctattg tctcaaagct ggattttaat aaatatattt cacaagcagg gctacttata
     9961 gcatcaacag caacaaaagt tggaagtttt ggagtaaata tattattggc atttttactg
    10021 agtttactcc ttttacttga aaaaaatgaa attaaaaatt ttggagataa actttctgat
    10081 agtaagattt cttttattta taattcgctt gtattttttg gaaagagctt tgttaaaaat
    10141 tttggtgaag taatgaaagt acaagttatg attgccttta taaattctgt tgtttctatg
    10201 attttcttag gtttcatggg attccctcaa atatgggctt taggatttat gatttttgtg
    10261 ttgggtctta ttccagtagc aggagttata gtgtctttga ttccattaac tgtaatagca
    10321 tttaatacag gaggaataac aaaggttttt ggagttttac ttatgatttg tatagtgcat
    10381 gcagtagaaa catacatatt aaaccctaaa ttgatgtcga atagaactaa acttccagtt
    10441 tgttttgttt ttattatttt acttgtagga gaacattatt taggtgtatg gggattacta
    10501 attggagttc caatatttat gttccttatg gatattctgg gtgttaaatt tacttctagg
    10561 taatatatta aaataataat ttatttttat atataaatta tgaattgttt taaaaaagaa
    10621 cctataaata gacatcatta ataaagacat ctatttgata ggttcaattt tgctttgtat
    10681 aaatactata aaactaaata aaaagaaatt tatttcattt caaaagtttc acaacttgca
    10741 tttctttcat ttatatgaac tttttcagct ttgcaaagtt catttttatt gtatttacat
    10801 tttacagctt cacaatgaat gttatctgta tcaacttggt tagtttcatt agaacgacta
    10861 gttataccag atgtatattt gtcttcaaaa gaagcacaat aagtattttc tgtagtagtt
    10921 gcttgcattc catctacttt tatactacca gcataacaat atccactagt attataagca
    10981 caattagtag cagcacaatt taaattatta tttttcataa ataacctcct aagttttatt
    11041 taaacttatt atgtgaagtt atctaaattt tatacactta tttacaatta catttatcac
    11101 atttaacaac tacatctttg gaagtttccc ttcctaaatt tttattaaca tgatacacat
    11161 caacattttc agttgatcca catatagaac atttaaaatt gttttctagt agggttcttt
    11221 ctttaaataa cttccaatac atagaattga tgtaatcatt ataatttacc ttgaggcatg
    11281 tctcaagaaa gtttatgtat agattgtcta aatctcctgt atttaattct gtatattctg
    11341 gttcaaaata ttcacaacat cctctaaaat cgggctttgt atctgaaata acacatctaa
    11401 tatcatatct atagtctagg ctaggcccct taaatgaaag atgtgtgcaa aaagaacaag
    11461 atgtttctgg tagatttttt tcaagttcat gtaatttatt attcataagt attcctccaa
    11521 aaaatttatt ttaagtatat aaacgaatag attattttaa aacttttatt tatttatatt
    11581 agagatttag gataaatatt ccaataagga gtaaatactt aagtatttat tactgtatta
    11641 aataaaaaag aggcaaatct aacttgagat tgcctacttt tttatcattt tttaataaaa
    11701 tttattctaa gtattatttt atttagattt agtcagccaa cttagtatct gctttgcttt
    11761 ttaactttct accagagcgt acagttaaaa tagcatataa tacagtaaat atggcagcta
    11821 aaacatatga tatatttatg tttaagttaa atccaatttg agcattaaat atataactga
    11881 atataacaaa ggaataaaac ataccaggaa ttaagcttat aatataattc ttttctttta
    11941 ttataaggta aactgtaatc attgcaaatg caaatatggc aatagtttga ttactccaag
    12001 agaagtatct ccacaatata ttaaatccag aagcactaag ctttgcaaat ataaggatgg
    12061 ctatgacagg aataaatata cagattgaca atataacacg atgtttcttt tccttttggt
    12121 cataatgaaa gtaatctgca agcattaatc ttaaagatct taaagctgta tctccagaag
    12181 ttattggtaa aacaattaca cctattatag ctatgatacc accaatagaa ccaagtaaat
    12241 ctcttgcaac caaacctatt acatcaggag aacctactaa ttccttaggt attcctttat
    12301 tgtaaatacc catagcagct gcagcccaaa tcattgcaat taaaccttct aaaatcatca
    12361 tattataaaa agtagttttt ccttcttttt catttgaaac tgatcttgct ataagagtag
    12421 cttgagttga gtggaatcca gaaacaatac cacaagcaac tgtaacaaag aatgtaggaa
    12481 taagaggaat accatcagga tgaatacctt tccaatttgc aagacttaaa ttagctaaat
    12541 cataaccttg tgtaaagatt ccaattccaa caccagcagc agataaaagt agaatagctc
    12601 caaatattgg atataccttt cctatgattt tatcaattgg aaatagtgtt gcacacaagt
    12661 aatatagaaa gataagtcca taaactatcc agacaactgg attatttata gtcgatttca
    12721 tatttaaaat ttgagtaaca actaaatccc caggtgtata aataaaaact gcaccaacta
    12781 aaagcattaa aagacaaagg aatatattat agacttgaaa tactttatta cctaaaaatc
    12841 tactgataag agaaggcatt tgtgctcctt tttctcttat actaatcatt ccactcatat
    12901 agtcatgcaa agctcctcca aatacacagc caataggtat tagtatgaat gctattggac
    12961 caaacaatat accttggata ggtcctagaa ttggtcctgt accagctata ttaagcaact
    13021 caattagact atttttccat tttttcattg ggacatagtc tactccatca ctttgagcaa
    13081 gtgcaggtgt ttttctgtca tctgggccaa atactttttc acaataagca ccgtataaag
    13141 cacctcctat tagtaatata gccaagccga ttaagaatgt tgtcataata tcaccccatt
    13201 tataaaattt tttggtcttt gtatacatat tatctaaatg tgtagattta tattgaattt
    13261 aattctggac aacctacaaa ttctataaag tattacaact aatataattt cagaaataaa
    13321 tacatataca taaccatgta cttagtatac aggtatttgg taatattttc aatgttatag
    13381 caataaagaa tataaacaaa attctgataa tattttacaa aaaaattaaa aaatataaaa
    13441 attagtttaa atagatggag aaaaaaggaa aatcaattga gaatataaat gtctatatct
    13501 taacaatatt aagaggttta aaattcttga agaaagaata gtcaagtgat ataatgagtt
    13561 tacaggtgta tatacactag tacatataga aagcggtgca attatggata aagatttaac
    13621 atatcaaatc aaagagctaa agaaagaaaa aaatgcaata atacttgccc atttttatca
    13681 accaccagaa atacaagaat tagcagatgc agttggtgac tcatattatt taagtgagat
    13741 tgctagagat tgtaaagaag aagtggttgt attttgtgga gtaaggttta tgggagaaag
    13801 tgctaagata ttatcacctg aaaaaactgt attgatgcca gtaagtaacg ctgggtgtgc
    13861 tatggcagat atggtagatg aagaaggcgt gataaaatta aaacaacagt atccaaatgc
    13921 tttggttgta tgttatataa actctacagc taaagttaag gcacattgtg atgtttctgt
    13981 tacatcttct agtgcaatta aaatactaga aaacatagat aataaagaaa ttatattcct
    14041 tccagataaa aatttaggag gatatattgc agaacaattt ccagataaaa actttatatt
    14101 ttgggacgga tattgtaaat accataacaa tataagagct gaagaaataa ttgagttaaa
    14161 agataagtat aaaaatgcag aagtattagt acatccagaa tgtaaaaaag aaataagaga
    14221 tttaggagat tatgtaggta gtacatcagg aataataaaa tatgctacta atagtaaaaa
    14281 caaagacttt ataatagcaa ctgaagaagg tatattacat gaattaaaga aaaataatcc
    14341 taataaaaac ttttatattc ctggtggaaa aatactatgt acagatatga aaaagacaac
    14401 attagaaaat ttatatagta cacttaaaaa tatggaaaat gaagttattg tagaagatga
    14461 aatcatggaa aaggctttaa attcactgct taacatgcac aaattagcag aaggatagag
    14521 ggatagactt atgaatttag aacaagatgt tttaatagtt ggaagtggag tttcaggatt
    14581 atactgtgca ttaaatttag ataaaagttt aaatgtgcta gtagtatcga aatcaacaat
    14641 tgaaaacaac aatacatatc ttgcacaagg tggaatttca actgctagaa atattgatga
    14701 tatagaatcc tttgtagaag atacaatgaa ggctgggcag tataaaaata gagttgaagc
    14761 agttcaagta cttgcagatg aatctataga aaatgtaggg cagatagtag aatatggtat
    14821 gcctttagac aaagaaaatg gagagataga ttacacaaga gaaggagcac atagtgtaaa
    14881 tcgaatagtt catagtaagg ataatactgg agaagttgtt tttaaaactt tattaaaaga
    14941 agctaaaact agagaaaata taactcttat agaagatgca tatcttttag atatattaaa
    15001 agatggcaat aaatgtatag gagctagaat ttttaaatct aaaaaagaga ttcatgtttt
    15061 ttcaaaaata gtagttttag ctacaggtgg aattggtggg ctttttaaaa attctactaa
    15121 tcaaagacat cttacaggtg atggaattgc aatagcttta agaaataata taaaaataga
    15181 aaatttagaa tatattcaaa tacatcctac agccttttat gaagaaaata atgaaggtag
    15241 gagaatgctt atatctgaat cattaagagg agagggagct aaacttttaa ataaaaataa
    15301 agaaagattt gtggatgaac ttcttcctag agatgtagtt tcaaaagcta tttttgagca
    15361 aatggaaaaa gataaattac cttatgtgta tttagatgca acacatttag atagtgaata
    15421 tttaattaat agattttctt ttatttataa tgaatgttta gctagaggaa cagacataac
    15481 gaaagaatgt ataaaggtat ctcctgcaca acattatttt atgggcggga tacatgtaga
    15541 tttggattca aaaacatcta tggaaaattt atatgcagtt ggtgaaatta gttgtacagg
    15601 tgttcatgga gccaatagat tggcaagtaa ttcattactt gaagggcttg tattttcaaa
    15661 aagagcagct aaaaatataa actcagttat agataatgta aaagttaagt ttatagatgt
    15721 accagatatg gatatagata tagaacaggt aaaaaaagaa aataaaatat tagtcataaa
    15781 agaaattgaa agaacaagtg aggattttgg cgatgaatta tttgattatt gatagaatga
    15841 tacaggatgc tttgatagaa gatgttccaa gtgaagatat aacaactaat tctattgtgg
    15901 atgaaaattc taaatctact gtagatttaa tttgtaaaca agatggtata atagctggct
    15961 taggtgtatt taagagagtt tttgagatac ttggtgatgt agatgtaaaa ttatataaaa
    16021 atgatggtga tagagtaaag aatcgtgaaa agatagctgt attaacaggt agtactagaa
    16081 acttacttgt aggagagcgt gtagcattga attatttaca gagaatgagt ggtatagcta
    16141 ctattacaag taggtatgtt gaaaagcttg aaggtacaaa tacaaaatta ttagatagta
    16201 gaaaaacgat acctaatctt agaatattag ataagtattc tgttaaagta ggtggaggat
    16261 gcaatcatag atttaattta tctgatggaa tattgcttaa agacaaccat attggggctg
    16321 ctggtagtgt taagaaggca gttgaattag ctagaaaaaa tacttctttt gttagaaaga
    16381 tagaagtgga agtggaaact ttagacatgg taaaagaagc aatagaagct aatgcagata
    16441 taataatgtt ggataatatg agtttggaaa tggcaaaaca agcagtagat attataaatg
    16501 gaagagccat tgtagaattt tccggaaatg taaatttaga tactatagaa gatataggaa
    16561 aaattggtgt ggatgttgtt tctgtaggag cgcttacaca ttctgttaaa gctctcgata
    16621 taagtatgaa gaatttgaga aatatataaa ttttatattg aaataaactt aattaaaata
    16681 gactccttaa atttgtttta taaatttaag gagtctattt tttattcatt aaaattaata
    16741 aaaatagaaa aaaatcaatc aataatttaa taaaaataga taaatactat atacactttt
    16801 aataatatag ttaatatcta ttttacagat aaataagaat aaacttataa tataattgta
    16861 tctgtaaaat tttgtaaaat ataaaaagta tagggggaat aaaaatgaaa gtagcttata
    16921 tttttagtag tactaataca cataaaattc tagacaaaat gataatacca caacttgaac
    16981 aaggaactca tggagtagac gttctaggga tgtttttctt tatggataat accttatttt
    17041 tatctaaagg taatcctgta ggcgagagac taagtaaatt acatgaaaaa actgatatga
    17101 taataatggc ttgtgaccaa tgtgctatag aaagaggtat agagaataat ttggttgatg
    17161 gagctacaat tggatgtttc ccaaacctat atgcttgttt aggcagtgca ggtgtggacc
    17221 aagttataac gttataaatt agtataggat acaatccata gaaaaattta ttttctgtgg
    17281 attattttaa gtaaaagata aattgtttaa agtaggggat taacaaatgt ctaaaatttt
    17341 agttgaattt ataaacactt gaccatcttg tgatgaacat ggagtgttta taaaaaaaat
    17401 gtcagaaaag tatgctgata aattggaaat aaaattatat caagcaggga aagatttttc
    17461 gtatataaaa aaatatggga ttataacaaa aggaacttta ataataaatc agaaaaagaa
    17521 atatgataga ttaaataaag atacaattga aagggcaata gtagaagcta taaacaataa
    17581 ttaaaagttt aaaaattagg agtatcaaaa atgaatatat tagaaattgt tttaaatgga
    17641 gttaaagata ctatagtagc ttctatagtt acactgaatg gagcatcagg atggcttatg
    17701 ataagtttta tattggcagg tgtacttcac aatgttttaa ctccaagaag gtttcaaaaa
    17761 caacttggaa ataagaaatt ttcttcttta attaaaagta ctatctcagg aatgttttta
    17821 ccaatatgta gttgtggagt aataccactt ggaataagta tgtattattc aggagcatat
    17881 ttaggaccag tacttgcttt tatgacatca actccaataa taaatccaat agctgtagtt
    17941 ctttgtctag gtcttttagg tcctaaagtt acaatcattt atctaatagc tggtttttta
    18001 gtaccatttt tagtaggatt tttaggaaat tgttttggaa aagaagaatt atcacttcat
    18061 aatgattcag aggaagaagt aatagaatta gaagaagaaa aaagaagttt tatgggaaat
    18121 atgattgatg gtcttaaatg gtcttttggt gaattagccc ttactattag taaatatgta
    18181 gtaatgggta tggtttttgc aggatttata acaactattt ttccaaatag tataattcaa
    18241 aaatatttag gaaatccagg tttgctatct cttggaagta tagcaatact tgcatgtgtt
    18301 atgtatgtat gtgctgtagg acatataccg tttatagcag cccttgttgc aagtggagca
    18361 tctcctggta tagcgataac atttctaata gctggagcag ctacaaatct accagaactt
    18421 ataagtatgt ttaaacttat tggaaaaaga actgttatta tatattctgt aactttgaca
    18481 ctgtcttcaa taataattgg atatataaca aatttacttt tagtgaataa taactcagta
    18541 aatttagatg caacaaattc ttcaatacag ttggcaaata aactaatgtt aaacatacct
    18601 gatagttttg agtatgtatg tactatttta attattttat ttgcatttaa atcaataata
    18661 ccaaagatga aagaggtcat aggaaatcat gcttaaaaag atagttataa tatcctgtat
    18721 agtagttgtg ttaatcattt tatcaaaaat agttgatgat aacatcaaag aagatgcttc
    18781 tattccaaat gtgaataaag aaacattaga atattttaga aaaaattata aagaagacat
    18841 aataacttgt gctgaagaag acttaaataa tgatggaaaa aaagatttag tagttatata
    18901 taaaaaaagc aataattcaa atgaaatggt agtcgtagtt tctgataaaa atagccacta
    18961 tataacaaag ccaataccag cacctataga gaatcaaact ataaccttta aaaatattga
    19021 tgataaagca ccaatagaag taattgtatc agggtctaaa aatggtaatg taggatatgc
    19081 tatttacaga gtagagggta aaaaatttgt tgatttattt ggcgaagata tggacaaatg
    19141 ttgctaaaaa taaaaataat acataaaggt gggaaattta tatgaaatta tctaagaaga
    19201 ttcaagcatt aatcttattt ggtgtaatgg gtacatctat attaacagga tgtagtagta
    19261 aaccaaaaga aaaagaagaa gcaaaaactg gaaaagatga ttatacaata acagtaggtt
    19321 attataattg tgaccatatg actgcgggac cagtagcaga atcggcagga atatataaag
    19381 atttaggtct taatgtaaaa actgttggaa atggtaaagt tcctgaagct atggcagcag
    19441 gaaaaatgga tgcaggttac ataggaacta aggggttagt tggagcaata cctaagggtt
    19501 caccaataac aatagcagct aacaaccata ctggtggttc tgaatattta atagtttcaa
    19561 aagatataaa agaacctaaa gatttaatag gtaaaaaaat tgcaacagat atgagtgatt
    19621 tcttatggac aagtgattat ggtccagaaa caggtctacc aactgaccca tcaaaatatg
    19681 aagttgtaaa tatggattca gacaaagata aatatttagc tcttaaaact gggaaaattc
    19741 aagcttttac atcttgtgac ccttggggtt ctgtagctga aaatgacggt gcaggaaaaa
    19801 taatagcatc tactcaatat aaagaaaaag ctaatggcaa agaatataac tgttgttcat
    19861 tctcattaaa caaaaacttt ataaaagaac atccagattt agcaaaaaaa ttggttctag
    19921 cacatacaaa agcaatagaa tatatatata caaatccagc tgaagcagct aaaatatttg
    19981 caaaatatta taatgtagaa gaagaagttg cacttagaac tatctataag aagactgttg
    20041 gagaaggtag aactcttact tggaaagtta caggtgaaga atataaaaat aatttacaaa
    20101 tgtataaaga tttaaaggct ttagatgata tacctaaata tgaagatacg attgatacaa
    20161 gcttattgga ctcttgtgga gctgatgatt ttgacaaatt tataaaagaa aaagttaacc
    20221 cagagtttcc tgaaggtatg agttatgaag actggaaagc aaaggtatta taaataaatg
    20281 gagaatatga aaaatataat aatacttttt atcattattt tgggaagtat atttataata
    20341 ttttttaatt ttgaagataa agaagaaata aaaggaaaat taagaatagg ggtatctgat
    20401 gatacctctg gttttgtaat aaattatatg gtaaatagag actactttga aaatataaaa
    20461 gtaagtgata taatggatat ttttacaatt aatgattgct gagcaagtac aatgcagtgg
    20521 gctctgagtt cagaggaaat agatatagca atcatatgta aagatgctgc aaaacaatat
    20581 gtaaatgtaa atagtggatt tgaaattgtt ggtactgtag ttcaaaattc tgatatattt
    20641 ctattaagtg ataataatcc tgaaaagata ggagtcattc aaaatagaga ttaccagtca
    20701 gaattggtta aaaaatacta caagaatgtg gaagtagctc ccttattagg gactgcacta
    20761 gcatatggat tagaaagtaa tttagtagat ggtgttgtaa tagattgcat aaagtcaatt
    20821 gggttagagg gcaaaagaaa atctacaact aaattaggag attatgatac ctatgtatta
    20881 gttgtaagta aagagtttaa aaaaagtaaa gcatatagtg attttataac actttacaat
    20941 aaaagtgttg atgctttaaa gaataaagct acatttaaga aagcattaaa agagtacagg
    21001 aatataagaa atatagatat ggaggaaata agcaattgga aattaaaatt tctaaagata
    21061 aaagcgatag attaactaag aaaaaaactg atggtagtat ggatatttta tataaaataa
    21121 tactattagt tattatattt ttagtctggc attttgcagc aaaagatata gggagttctt
    21181 tgttacttcc aatgccagta gatgtaataa aaggtttctt tttctgtgtg acagatgcag
    21241 agacagtcac taatcttttt ataacattgc agagagtact taaaggtttt atgtatgcat
    21301 tattgtttgg gcttccaata ggatttatta tgggattttc taaaactttt gaaagggttt
    21361 tatctccagt agtagattct gtaaggcaag ttccaataat ggcatgggtt cctcttacta
    21421 tagtatggtt tggtatagga gatggtccaa caatattttt aatagctttt tcaggggttt
    21481 tccctataat tttaaatact attcaaggag taagagcaat atcaaaggat tattataatg
    21541 cagctagaag tatgggagca tcgccaattg tgatatttac aaatgtaatt gttcccgcat
    21601 ctcttccaga tatactaact ggttcaagga ttgctataag tacaggatgg atgtctgtta
    21661 tctgagccga gtttatagct acgagtgccg gtctcggtta ctcaatggta caagctcaaa
    21721 cattaatgca tacagatgta ttaattgggc ttatgatatt tgcagcttta ataggattta
    21781 taatagatag ggtattaaag tttataaata aaatcctttg tagatggagg tttgcagatt
    21841 aatgaagtta agtgtaagag atataaataa gacatttgta aataatagag tacatacaaa
    21901 ggttttagaa gatataagta tagatataga tgatggacaa tttgtctgtc ttttaggacc
    21961 atcaggttgt ggaaaaacaa ctttacttac tataataggt gggtttcaaa aatcagaacg
    22021 tggagatgtt tttataaatg ataaaagagt taaaaaaccg ggcatagata gagcttttat
    22081 atttcaaaat tatgctttat ttccttggaa aactataaga ggtaatgttc tatttcctat
    22141 gaaacagcag aaaataccaa aagaaaaaag agaagaaatg ctagaagaac ttttggttat
    22201 gtcagacttg aaaggaaaag aaaaactgtt tccgcatcaa ttatctggag gaatgaaaca
    22261 aagagtggcc atgataagag ctttagcatg taatccagag gtattactaa tggatgaacc
    22321 attaggagct gtagattttc aaatgagaca aaatttacag gaagagctag aacgaatatg
    22381 gataaagaag aaaataactg cattaatggt aactcatgat gttgatgaag ctgtatatat
    22441 gagtgataga gttatagtaa tgtcgagaga taaaggaaga attattgatg atataaatat
    22501 agatattcca agacctagaa ttagaggaag ccaaaaatat gaagagtaca aaaacaaatt
    22561 aactgatact ttatcaaagt gttatgaagt ttaaagagga gtagacatga gtcaaataga
    22621 aaaatacaat agtttggata atacacaaca gaaaataaga atctctatta tggatatgat
    22681 aatagataaa ggttctagtg ttacacttca agaagtgact gaatatgtaa gtaaaaagtt
    22741 gaatatagaa aaagaatata tagaaaggac actacaatat tttatttata aaaatataat
    22801 ggttgttgat gggaatagta taaattttat atatcctgta tcagcattgc caacaaatca
    22861 taaagtaact ttgagagata atagaagttt cagtgcaatg tgtgctatag atgctatagg
    22921 aacctcttgc acattcaacc aagatataaa aataaactct atttgtagtg ctacaggtaa
    22981 ggaaatagaa gtaattataa aaaatgaaaa gattgagtat gtgaataatc caaacttaag
    23041 agtgcttcat ataaatcttg ataagcatct aaattgggct gctagttgct gaaatataat
    23101 gaatttcttc tggacgaaga gtgattttga tgcttggaca aacgaagcgg gattatctga
    23161 tgatgaagat atatattgtt tagatattaa tgaggcaata gtagaatcat ataaaatatt
    23221 taaactcaaa caaaaggtac tatcatagat atacatatat gaaaaactaa aaatgacctt
    23281 tagataaaaa tgataggtaa tatccatact atataaatta gtatagagta gggatgtgaa
    23341 aagtatttaa atctaaaggt atatttttag tttttttaga tgattagata gccttattaa
    23401 gtatatattt atttataacc tttgcaactc cattttcatt attagtatca gtaacataat
    23461 ctgcaatttc ctttatacta ttacgtgcat tccccatagc cacaccaaga cctgcatact
    23521 ttatcatatg ttggtcattt tcttcatcgc caacagcaat agcattttct ataggtatat
    23581 ttagattcgt acataatgtt ttaagagcag ttcctttatt agtagtttta cctaaaaatt
    23641 ctagataaaa aggagcagac cttactatag tatatttatt gaataaattt ttaggtagtt
    23701 gctgtatgat ttcttccaat ctttctggtt catcaactat cataatttta cagattttaa
    23761 tagattcatc aattttatct ttttgtatat atttgacttc tatattattt aatttcgctt
    23821 catgaagtgt atatttgcca ggcgtttcat ttggtgtaat taaatttgtc attgtattta
    23881 tgtgtatttt agtcttatat tttttacaaa cgttattata tataaaatca aaatcatcta
    23941 aagtcatttc tacatcatgt atgattttct cacttttaac ttcttgaata attgctccat
    24001 taaagcatat aacataatca ccttcatctg ttaaatttag ttgatttaga agttctttta
    24061 caccaggaag aggtctgcct gttgttataa ctacttttgc cccagccctt tttgcctcct
    24121 gaatggactc aaagacttca ggagtaatcc ttttttgtgt atttaatatt gttccatcaa
    24181 tgtctagtgc gattaatttg tacatatcaa cctccaataa attatatact caaatttcct
    24241 gtgaaaacgt tgacacagct agtgttgaac aaagtttcag tgggaaatat ggtttgtttt
    24301 gtatactatt atatcaagag ataagtaatt tagaaagttt aataatatta attttttgat
    24361 aaaaatatta cttatgtgag atgagaatgt aaaaactttg taatcaataa ttctaaaatt
    24421 cttaattaca aataatggta tcgtttgcat tgttaatata aataaa
//
LOCUS       DAFKMX010000041        24080 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFKMX010000041 DAFKMX010000000
VERSION     DAFKMX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.041"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            179..1660
                     /locus_tag="LZB08_001335"
     CDS             179..1660
                     /locus_tag="LZB08_001335"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBM7314845.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            1881..2036
                     /locus_tag="LZB08_001336"
     CDS             1881..2036
                     /locus_tag="LZB08_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314846.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            2181..3578
                     /locus_tag="LZB08_001337"
     CDS             2181..3578
                     /locus_tag="LZB08_001337"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBM7314847.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFVK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            3563..3976
                     /locus_tag="LZB08_001338"
     CDS             3563..3976
                     /locus_tag="LZB08_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBM7314848.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            4082..4840
                     /locus_tag="LZB08_001339"
     CDS             4082..4840
                     /locus_tag="LZB08_001339"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBM7314849.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            4853..5590
                     /gene="rlmB"
                     /locus_tag="LZB08_001340"
     CDS             4853..5590
                     /gene="rlmB"
                     /locus_tag="LZB08_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBM7314850.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            5593..6120
                     /locus_tag="LZB08_001341"
     CDS             5593..6120
                     /locus_tag="LZB08_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBM7314851.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            6185..6832
                     /gene="sigH"
                     /locus_tag="LZB08_001342"
     CDS             6185..6832
                     /gene="sigH"
                     /locus_tag="LZB08_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBM7314852.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            6906..8099
                     /gene="tuf"
                     /locus_tag="LZB08_001343"
     CDS             6906..8099
                     /gene="tuf"
                     /locus_tag="LZB08_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBM7314853.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVE
                     DVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGD
                     NIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVV
                     ATIIE"
     gene            8349..8498
                     /gene="rpmG"
                     /locus_tag="LZB08_001344"
     CDS             8349..8498
                     /gene="rpmG"
                     /locus_tag="LZB08_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBM7314854.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            8524..8745
                     /gene="secE"
                     /locus_tag="LZB08_001345"
     CDS             8524..8745
                     /gene="secE"
                     /locus_tag="LZB08_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBM7314855.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            8765..9307
                     /gene="nusG"
                     /locus_tag="LZB08_001346"
     CDS             8765..9307
                     /gene="nusG"
                     /locus_tag="LZB08_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBM7314856.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            9346..9771
                     /gene="rplK"
                     /locus_tag="LZB08_001347"
     CDS             9346..9771
                     /gene="rplK"
                     /locus_tag="LZB08_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBM7314857.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            9842..10540
                     /gene="rplA"
                     /locus_tag="LZB08_001348"
     CDS             9842..10540
                     /gene="rplA"
                     /locus_tag="LZB08_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBM7314858.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    10592..10720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            10763..11269
                     /locus_tag="LZB08_001349"
     CDS             10763..11269
                     /locus_tag="LZB08_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBM7314859.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            11327..11692
                     /gene="rplL"
                     /locus_tag="LZB08_001350"
     CDS             11327..11692
                     /gene="rplL"
                     /locus_tag="LZB08_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBM7314860.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            12084..12872
                     /locus_tag="LZB08_001351"
     CDS             12084..12872
                     /locus_tag="LZB08_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBM7314861.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            13343..17059
                     /gene="rpoB"
                     /locus_tag="LZB08_001352"
     CDS             13343..17059
                     /gene="rpoB"
                     /locus_tag="LZB08_001352"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBM7314862.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            17101..20586
                     /gene="rpoC"
                     /locus_tag="LZB08_001353"
     CDS             17101..20586
                     /gene="rpoC"
                     /locus_tag="LZB08_001353"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBM7314863.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALEIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            20875..21297
                     /gene="rpsL"
                     /locus_tag="LZB08_001354"
     CDS             20875..21297
                     /gene="rpsL"
                     /locus_tag="LZB08_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBM7314864.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            21421..21891
                     /gene="rpsG"
                     /locus_tag="LZB08_001355"
     CDS             21421..21891
                     /gene="rpsG"
                     /locus_tag="LZB08_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBM7314865.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            21940..24006
                     /gene="fusA"
                     /locus_tag="LZB08_001356"
     CDS             21940..24006
                     /gene="fusA"
                     /locus_tag="LZB08_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBM7314866.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         9654 a         2824 c         4866 g         6736 t
ORIGIN      
        1 taaatgactt aaaagaaaga tttatgaaat taaaactagg attttctgat ataataaaca
       61 tgtgtatttt taaataaaaa ggatattatt attagatttg attaaaaata aacttgcaaa
      121 acaatgtact agtttaatat tatagttata ttgaataaaa tatcaggagg aagtaaaaat
      181 gaatgtaaga gtaaggtttg caccaagtcc aacaggattt gtacatatag gtagtttgag
      241 aactgcttta tataactatt tatttgcaaa aaaaatgggc ggagaatata tattaagagt
      301 ggaagataca gaccaaagta gattagtaga aggtgctata gagaatatgc ttaatgctat
      361 gaaatgggct ggggtcaacc atagtgaagg tgttatatta gatgatagtg gaaaagtagt
      421 acaaaaagga gaatatggtc catatattca atctcaaaga ttagatatat accaagaata
      481 tataaagcaa cttttagata gtggaaaagc ttattactgt ttctgtacaa aagaaagact
      541 ggatgaagta agagatgctc aaagagcagc aggagaaaca gctaaatatg atggtcattg
      601 taagaatctt tctaaagaag aagtagaagc gaatataaaa gcaggaatac catatgttat
      661 aagattaaga ttgccagaga atcatacaat taagtttaca gacttagtta gaggagatat
      721 ggaatttaat actaatgact tagatgacca agtattaatg aaaacagatg gatttccaac
      781 ttatcatttt gcagtagttg ttgatgacta tttgatgaaa attacacatg ttataagagg
      841 agaagaatgg gtatcatcta ctccaaaaca tgtttattta tatgaagctt ttggatggga
      901 agcacctgta tttgtgcatt taccaaatat acttaataaa gaaaagaaaa aattaagtaa
      961 aagacaaggt gatgtagctg ttgaagactt taagaaaaaa ggatatttac cagagggatt
     1021 agtaaactat gtagcattag ttggttggtc tcctgaagat aatcaagaat tgtttacaat
     1081 ggaggaatta gaaaaagcct tctctgtaga aagggtatct aaaagtggtg gagtatttga
     1141 tactgaaaaa ttaaattggg ttaatcagca ctatataaaa gatggtgatg atgcttatct
     1201 aacagactta gcaataccat ttttaataga agatggattt ataacagaag aagaagctac
     1261 taataaatat gatttcttaa agagtatgat atcagtactt aaagagaaat tacaatatgt
     1321 aaaagaagtt actgaacatg caagtatatt ttttggagat aaaatagagg tagaaactga
     1381 agagggaaga gagttcttaa gattagaaca tatacctact ttaatagatg ctttgagaga
     1441 aaaaatagaa aaaacagagg tattaaatgc tgagtttgtt caagctatgc ttaaagaaat
     1501 acaaaaagag tacaaaataa aagggaaaaa tttatttatg ggctcaagaa taattttgac
     1561 agggcaaatg catggtccag atttaccaaa agtaatggaa gttttaggaa aagaaacttg
     1621 cttaaataga atagcttatg taaaaaataa tatattatag tcataaaagt ccgctaattt
     1681 agaagcggac tttttttgtg ttagtatttg gtgaaaatat ctgcttaaga aaatgaaggt
     1741 atttaaggta ataaatttaa tatataaaaa gcaaagcaaa aatataaata aaatatgaat
     1801 aataaactaa taaaatatta agcatagaag aagggaatct attattttag tttgatttaa
     1861 ttattaaggg ggtaattact atgtatagta atattgatga tgttaaaaag gaattaaagg
     1921 agctatgctt agaatatgtt acaattttag aaaaattaaa ggatgaaaaa atgattacag
     1981 aagaaacttt tgaaaagtgc tcttctcaaa aaaaaatatt tttagaagaa caatagagga
     2041 tggtaatagt attattatta tgtagtttga aagatagtac gaaaaatgat agtaatacta
     2101 ttatttattg caattttcaa ctataatata atatataatg ttaagaagat aatttataaa
     2161 aatacagtga ggtgatgagt gtgaaagttt ataatacatt gactagaaca aaagaagaat
     2221 ttgttccatt agaagaagga aaggtaaaaa tgtatgtgtg tggtccaaca gtttataact
     2281 atatacacat aggtaatgca agaccattta taatatttga tactctaaga aggtatttag
     2341 agtatagagg atatgatgta acttatgtac aaaactttac agatgttgat gataagatta
     2401 taaatagaag tcatgaggag ggaatatctc ctgaagaagt tgcggctaag tatataaaag
     2461 aatattttgt agactgtgat gggctaggaa taaaaagagc tactgttcat cctcaggtta
     2521 cagataatat acaacagatt atagagttta taaaagagtt ggaagataag ggatatgcat
     2581 atgcagtaaa tggtgatgta tattttgata caaataagtt tgaaggttat ggaaaacttt
     2641 caggtcaaaa acaagaagat ttagaggcag gggctagaat tgaggttaat gaccaaaaaa
     2701 gacatcctat ggactttgtt ctttggaagg caaaaaaaga aggtgagcca ggatgggata
     2761 gcccttgggg agaaggtaga ccaggatggc atatagaatg ttctgttatg tctaagagat
     2821 atttaggtga aacaattgat atacatgcag gtgggcaaga tttaacattc ccacatcatg
     2881 aaaatgaaat agctcaaagt gaagctagaa gtggaaaaac attctcaaaa tactggatgc
     2941 ataatggata tataaatata aatgatgaaa agatgagtaa atcaaaaggt aatttcttta
     3001 cagtaagaga tatatcaaaa ttatatgatt tagagatagt aagatttttc atgttatcag
     3061 cacattatag aaatccagta aactttagtg atgagatgtt aaatcaagct aaagcaggtc
     3121 ttgaaagact atataatact aaagaaaaat tagaatttac gctaagtaat ttagtagaat
     3181 ctcctttaac agaaaaagaa gtagagcttg ttaaagaatt agatgacttt agacaaaaat
     3241 tcatagatgc tatggatgat gatgttaata cagcagatgc agtaagtgta atatttgaat
     3301 tagctaagtt aataaattct aatgtagatg aaaattcttc tttggagttt gttaaaaaat
     3361 gcttagatga atttaatgaa cttacaggag tattaaatat tgtaaataag aaaaaggata
     3421 ctgtattaga taaagacatt gaagaattga tacaaaaaag aactgatgca aagaaaaata
     3481 aagagtttca attagctgat gatattagac aacaattact agatatggga attgtattag
     3541 aagacacaag acaaggtgtt aagtggaaaa ggatataaaa atggaaaaaa ctgaattagt
     3601 tactatgtca ccattagttt tagcatactt aggtgataca gtgtatgaga catatataag
     3661 agaatattta attagacaga atacacaaag aaaagttaat gatttgcata aactagctat
     3721 aaagtatgta aaagctaaag ctcaggcaac tataattcat gaagttgaaa ttgagttaac
     3781 tgaagaagag agtaaaattt ataaaagggg aagaaatcaa aagtcaaata cttctcctaa
     3841 gaatgcagat attatagact acaaacatgc aactgggttt gaggcattag ttggatattt
     3901 gtatttaaat aatgaaatag agagacttca atatattatc aataaaggaa tcaaaattat
     3961 tgaaagagat atgtaaatat taaagagtgt tgtcttaata ataagtaata aggcaacact
     4021 ttttttataa ctaatgtata ataaatatat agaaattagt aatgagaaag gataaaaagt
     4081 tatgaaagta aaattaatat cacatacacc tgaaccagaa aaagtaatag ctatggcagc
     4141 taagctatgt tattcaccag ttgggacaga cgaaatagaa aaagacttaa cagatgaaag
     4201 tatagagaaa tttttaaata tgttattaag tataggtcat gggtcaatat tagaacatgc
     4261 ttcatttaca ttttcaatag aaggtatttc aagggcatgt tcacatcaaa ttgttagaca
     4321 tcgtatagct agtttttcac aacaaagtca aagatatgtt aaattagaac aatttgagta
     4381 cataattcct ccagaaatag aaaaaataga aaaggcaaaa gagttattta tagactccat
     4441 gaaaaaagac caagagaatt atgataagtt agttgaaata ttgtttgaaa atcattataa
     4501 tgacctaata aaaaacggta aaaatgagaa aacagctaaa agacaagctg agaagaaagc
     4561 aatagaagat gctagatacg tatttccaaa tgcatgtgaa acaaaaatgg tatttactat
     4621 aaatgctaga agtttgttta atttctttga acatagatgc tgtgaaagag cacagtggga
     4681 aataagaaat ttagctgtgg agatgttaag agaggttaaa aaggttgctc ctatattatt
     4741 taaaaagact ggtccaagtt gtgtaaatgg aagttgtcca gaaggaacta tgacttgtgg
     4801 agatatagtt caagttagag aaaaatttaa ggccttgtag gaggtgaata atttggcgag
     4861 tatagaagga agaaatccgg taatagaagc aataaaaagc gacagagaaa tagataaaat
     4921 attaattgca aattcagcta aagaaggttc aattaagaaa ataataggaa tggctaaaga
     4981 taagaatata attattcagt atgttgataa acataagcta gatgaagtaa gtacaagtca
     5041 ttcacatcaa ggtgtaatag cttatgcaag tgagtataag tattatgaat tagatgagct
     5101 aatagattta gcaaagaata aagatgaaga cccatttttc attatattag atgagataac
     5161 agacccacat aatttgggtt caataataag gacagcagat gcagtaggag cacatggagt
     5221 tatcattcca aaaagaaggt ctgtacacat aactcctgta gttgccaaag catctgctgg
     5281 agcagtagaa tatatgccag tttgcaaggt aaccaatata gttaatacaa taaaaagact
     5341 caaagaagaa gggctgtgga ttgctgcagc tgatatggat ggagaaactt tttataaaca
     5401 aaatcttact ggtcctcttg gtgtagtaat tggaagtgaa ggttttggaa tatctagatt
     5461 ggttaaacaa aactgtgact ttatagtaaa aatgccaatg ataggtaatg ttacatcttt
     5521 aaatgcatct gtagcaggtg gaatactttt atatgagata ttcagacaaa gactggataa
     5581 aagtaaatag gcatgaaaaa aaatataaat cattacttaa taatagatgg ctacaatatt
     5641 ataaatgcgt gggacaattt gaaagaactg gctaaagagg acttagaaga ttctcgagaa
     5701 aagcttattg atgacattat agaattttct gagtttatgg gttataagac tataatagtt
     5761 tttgatgctt ataatgttaa aaattctcga gaaaaagttg aaaaaagaaa acacataaca
     5821 atagtttata caagagaaca ccaaacagca gatagttata tagagaaatt tataacttct
     5881 ttatcaaagt atgatgatgt aaaagtagcc actaatgact atgcagaaca gcaaatgatt
     5941 ttaggaaaag gtgctactag aatgtctgca agagaattaa agttagagtt agatcgttcc
     6001 aagaataaaa tgaaggaaaa aaatatcggt ttgaggaaaa aaattcagcg aaattggttg
     6061 gaggaaaggt tagataaaga aacattgtcg aaacttgaga acattcgtag aaagcgttga
     6121 aattactagg ttctttagac tataatatac ctataaattt gttttatata tgggggagat
     6181 ttacatgttg gtagcaaaag aaaaaagtta tgagttagtg gacaattgtc agcaggatga
     6241 gtataacatt gtattaagag caagtgaagg ggataaaata gcactagagt acattattac
     6301 taaatacaga aattttgtaa aggcaaaagc aaagtcatat tttttaattg gagcagataa
     6361 agaagatata atacaagaag gaatgatagg cctttataag gcagttagag attttgatgg
     6421 aagtaagacc aattcattta aatgttttgc ggagatatgt ataacaagac aaataataac
     6481 tgctataaaa actgcaacaa gacaaaaaca tataccttta aattcatatg tttctttgaa
     6541 taaaccaata tatgatgaag agtcagacag aacactttta gatattatag ctacaagtat
     6601 agtaacagat ccagaagagc ttataattag taaagaagag ttaaaaaata tagaatcaaa
     6661 aatgaacgaa ttgttaagtg acttggaatt agaagtacta gaattatatt taaatggaaa
     6721 gtcataccag tttatagcgg acaagcttaa aagagatgta aagtcaatag ataacgcttt
     6781 acaaagggtt aagagaaaat tagaaaaaca tcttgaaaac agaaatgatt aatagtaaaa
     6841 tacttaccat aagcttattg tgagcaaaat taatttatat cgaaagataa gggaggaaat
     6901 tcaaaatggc taaagctaaa tacgaaagaa caaaacctca tgttaatata gggacaatag
     6961 gacacgtaga ccacggtaaa actacattaa cagcagcaat aacaaaaaca ttatatgaca
     7021 gatatcaatt aggagaagca gtagatttcg caaacataga taaagctcca gaagaaagag
     7081 aaagaggaat cacaatatca acagcacacg ttgagtatga aacaccaaat agacactatg
     7141 cacacgttga ctgcccaggg catgctgact acgttaagaa catgataaca ggagcagcac
     7201 aaatggacgg agcaatatta gtttgttcag caacagatgg accaatgcca caaacaagag
     7261 agcatatact attatcaaga caagttggag taccatatat agtagtattc ttaaacaaat
     7321 gtgacatggt agatgatgaa gagttattag agttagtaga gatggaagta agagatttat
     7381 taacagaata tgatttccca ggagatgaca ctccaatagt aagaggttca gcattaatgg
     7441 cattagaaga tccaaagagt gagtggggag ataagatagt agaattattc gagcaaatag
     7501 atgagtatat accagctcca gagagagata cagataaacc attcttaatg ccagtagagg
     7561 acgtattctc aatcacagga agaggaacag ttgcaacagg aagagtggaa agaggagtac
     7621 taaaagtaca agacgaagta gagttagtag gattaacaga agcaccaaga aaagtagtag
     7681 taacaggagt agagatgttc agaaaattat tagaccaagc acaagcaggg gataatatag
     7741 gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta ctagcaaaga
     7801 ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt aaaaaagaag
     7861 agggtggaag acatacacca ttctttgatg gatatagacc acaattctat ttcagaacaa
     7921 cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg cctggagata
     7981 acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga ttaagattct
     8041 caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata atagagtaat
     8101 tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc tcctcttttt
     8161 ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac ttatacaagg
     8221 taatgattat aggaagttaa taatatcttg aatactgtag atttttaata aatacatatt
     8281 ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca aagttggagg
     8341 tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa agaaactaca
     8401 acactactaa gaataagaaa aacaatccag acagaataga attacaaaaa tattgtagat
     8461 tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg atgtgagtat
     8521 ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag tttgtttgga
     8581 tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa aaaggaactg
     8641 tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt agtatggggt
     8701 atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa aagaggtaag
     8761 ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt cgggacatga
     8821 aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg aagactgcat
     8881 aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg gaaaagaaaa
     8941 aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa taactgatga
     9001 atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac ctggttcaaa
     9061 accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa cagaccctaa
     9121 agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat ctcaaggacc
     9181 atttagtgga caaataggta acatagaaga aattgactta gaaaatagag aagtcaaagt
     9241 atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag gtatagaaaa
     9301 aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc taaaaaagtt
     9361 ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc accagttgga
     9421 ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt taatgctaaa
     9481 actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca agatagatca
     9541 ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc attaaactta
     9601 aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc agctcaagta
     9661 agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga agctgctatg
     9721 agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta attaaaagat
     9781 acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg aggaaaataa
     9841 aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata gaactaaatt
     9901 ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta aatttgatga
     9961 gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc aacaagtaag
    10021 aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag tttttgctaa
    10081 aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag ctgaagaatt
    10141 agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg ctactccaga
    10201 tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt taatgccaaa
    10261 ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa taaaagctgg
    10321 taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag gaaaagtatc
    10381 atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta taataaaagc
    10441 taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt cttcaatggg
    10501 acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt gacacaggtt
    10561 gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc cgtagatagt
    10621 gggtgcgaga gcttaatatc ccgccgaggt tttagataaa ttgagattac taaagctttt
    10681 gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact aagcctattt
    10741 ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag aagttgtttc
    10801 tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt acaaaggatt
    10861 aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg tagattacaa
    10921 agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag agcaatttaa
    10981 tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc cagttgctcc
    11041 agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa agatgggtat
    11101 tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca taccatcaag
    11161 agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa acttcgcata
    11221 cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat tgcttaacta
    11281 aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga caatagaaca
    11341 aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag ttaaagctgc
    11401 tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg cagctgctgg
    11461 tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg ttggttcttc
    11521 aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa aagaagctaa
    11581 agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag aagaagctga
    11641 tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt agtttcttta
    11701 aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa agtatttgga
    11761 attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac taaattaagg
    11821 gtatcatata tggtgtaatg gtattttaat ttaacattat tatcaaatta aaatataaac
    11881 aattgttgta atgtctgtat ataaagtata taattaaact aaaatgtaaa attttacatt
    11941 ttagtttaaa aaaatacacg agtgttttgt aaataaaatg taataaataa ttaattaaca
    12001 aatataaatt ttaaaataag ttagatattt agaaaaatcg aaatttgtag gatttaaagg
    12061 acatattcaa ggaggagtta ataatggaaa aattacaagg aaaaattgca gtagttactg
    12121 cggcaacaaa aggtattgga ttagcatcag cagagatatt agcaaaaaat ggagcaactg
    12181 tgtacttagc agctcgttca gaagaattag ctaatgaagt tataaataaa ataagtgcag
    12241 aaggtggttg tgctaagttt gtttacttta atgctcgtga agaagagacc tttacctcaa
    12301 tgatagaaga ggtagttaaa aaagaaggta agatagatat acttgtaaac aattttggtt
    12361 caacaaaccc ttctcttgac aaagaccttg taactgggga tacagataat ttctttgaca
    12421 cagtaaatac taatttaaaa agtgtgtatt taccatgtaa agcagcaatt cctcatatga
    12481 taaagaatgg aaaaggaagt atagtaaata tatcaagtat aggttcagta ttacctgatt
    12541 tatctagaat agcttactgt gtatcaaaag cagcaatcaa ctcattaact caaaatatag
    12601 ctacacaata tgctaaagac aatgttagat gtaatgctgt acttccaggg cttattgcaa
    12661 ctaaagcagc attagacaat atgtctccag agttcataaa agaattttta aagcatgttc
    12721 ctttaaatcg tataggggaa ccagatgata tagcaaaagc tgttctattc tatgctagtg
    12781 atgactcatc atttataaca ggggatttac ttgaagttgc aggaggattt ggtttaccta
    12841 ctccacaatt tgcagataat atattaggat aattaaatta ctaatatgca atgaaagctt
    12901 atgcattagt tatagtagaa atgatatttt tataaaggcc atagatataa tttatattgg
    12961 aaaggtttga ggttcaaaat tccaagttaa atttatttat ggtctttatt ttgtatattt
    13021 taataaattg tatatatttg tataaatgta attatttaca atcattttaa tatggaatta
    13081 taattaaata ggggggatgg tacttgtata aaatttttta tagaaaatta gacctataaa
    13141 tgaaaaatat ggattttata ttggaaaatt gtacataata cataatatat gcttaaaata
    13201 atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat atattgaaaa
    13261 atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt tttattttta
    13321 attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa gaactagaat
    13381 gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa ttcaagtaga
    13441 ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata tttcaccaat
    13501 agaagattat actggtaatc ttatattgga atttgtcgac tattctctag atgataaacc
    13561 aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac ctctaaaggt
    13621 taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag tgtttatggg
    13681 tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag aaagagttat
    13741 agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag ataaaacagg
    13801 taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag aatatgaaac
    13861 agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac cagttacagt
    13921 tttacttaga gctttaggaa taggaacaga tgcagagata attgaccttt taggagaaga
    13981 tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag aaggtcttgt
    14041 agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg catcatcttt
    14101 attaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg gtagatataa
    14161 attcaataag aaacttgctt tatgttacag aataatgaat aaaatttcag cagaagacat
    14221 aataaatcca gaaactggag aagtatttgt aaaagctgga gaaaaaataa gctatgattt
    14281 ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg atgatgataa
    14341 aaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg attttgacat
    14401 agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa ttttagatgg
    14461 atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag aacttatacc
    14521 aaaacatata ttattagatg atataatagc ttcaataagt tatgagttca atatattcta
    14581 taatatagga aatattgatg atatagatca cctaggaaac agaagaataa gatctgtagg
    14641 tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag ttataaaaga
    14701 aagaatgacg gttcaagata tggaagctat aacgcctcaa gcgttagtta acataagacc
    14761 agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt tcatggatca
    14821 aacaaatcct ctatctgagt taacgcataa gagaagatta tcagcccttg ggcctggagg
    14881 tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt caccattcac actatggtag
    14941 aatgtgtcca atagagactc cagagggacc aaatatcggt cttataaact ctctaggaac
    15001 ttatgcaaaa ataaatgaat ttggatttat agaatcacca tatagaaaat ttgataaaga
    15061 aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag atttattcgt
    15121 aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc atagagttgt
    15181 ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag attacatgga
    15241 tatatctcct aagcaggttg tatctgttgc cactgctatg atacctttct tagaaaatga
    15301 tgatgccaac cgtgccctta tgggagcgaa catgcagcgt caggcagtgc ctctagttag
    15361 aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag actctggagc
    15421 agttgttgtt gctagaaatt ctggtatagc agaaagagta acagcagatg aaataattat
    15481 aaaaagagaa gatggaaaca gagatagata taatctactt aagtttaaac gttcaaactc
    15541 aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagataa taaaaggtga
    15601 tgttatagca gatggtccag caactgattt aggagaagtt gcacttggaa gaaactgtct
    15661 tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa taaatgaaag
    15721 attagttaaa gaagatagat tatcaacaat tcatatagaa gaatatgaat gtgaagctag
    15781 agatacaaaa ctagggccag aggaaataac tagagatata cctaatgttg gagatagtgc
    15841 aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag actcaggtga
    15901 tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa cttactgctg aggaaagatt
    15961 acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac ttaaagtgcc
    16021 tcatggtgaa tctggtataa tagttgatgt aaaagtattt actagagaaa atggagatga
    16081 tttatctcca ggagtaaatg aattagtaag atgctatata gctaaaaaga gaaaaataaa
    16141 agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa gagtattacc
    16201 tgaagaagat atgccattca tggaaaatgg aacaccacta gacataatac ttaatccaca
    16261 aggtatacca tcacgtatga acattggtca ggttttagaa gttcatttag gacttgctgc
    16321 taagacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg agtatgacat
    16381 aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt tatatgatgg
    16441 tagaacaggt gagtcttttg acaacagaat aactgttgga tatatgtact acttgaaact
    16501 acatcactta gtagatgaaa aactacatgc aagaagtact ggaccatact cattagttac
    16561 tcaacagcca ctaggtggta aagcacaatt tggtggacaa agatttggag agatggaggt
    16621 ttgggctctg gaagcatatg gagctgctca catacttcaa gaaatactta ctgttaagtc
    16681 tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg aaaatatacc
    16741 tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa gtttatgctt
    16801 agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat ctgtggatga
    16861 agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag aagttgaaga
    16921 aagtaatata atagaagaaa ttgaagatga ttttgcagaa aatgcagaag atgaggatat
    16981 agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag attttgatag
    17041 tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg agagaactcc
    17101 ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc agaaaaaata
    17161 agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg tactttaaaa
    17221 ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga ctgggagtgc
    17281 cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag atgtggagta
    17341 gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct agctgctcct
    17401 atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt acttgatatg
    17461 tcaccaagat cgttagaaaa aatattatac tttgcctcat atgtggtagt tgatccagga
    17521 gagactggat taaatgaaaa acaattgctt acagaaaaag aatacagaac tgctcttgaa
    17581 aagtatggat atacttttac tgtaggaatg ggtgctgaag ctgtaaagac attactacaa
    17641 aatatagatt tagagcaaca aagtaaagac ctaagagcgg agttaaaaga tagtacaggg
    17701 caaaagaaag ttagaacaat aagaagatta gaagttgtag aggcatttaa aaagtctgga
    17761 aataaaccag aatggatgat tttagatgca ataccagtaa taccaccaga tttaagacca
    17821 atggtacaac ttgatggtgg aagatttgcg acttcagacc taaatgattt atatagaaga
    17881 gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc agatataatt
    17941 gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cattgataga taatggtaga
    18001 agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc agatatgtta
    18061 aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga ctactcagga
    18121 cgttctgtta tagttgttgg accagaactt aaattttatc aatgtggact tccaaagaag
    18181 atggcattag aattattcaa gccatttgtt atggataaat tagttaaaga aggttatgca
    18241 cataatataa aaagtgcgaa atctatagta gaaaaagtta agccagaagt ttgggatgtt
    18301 ttagaagatg ttataaaaag tcatccagtt cttcttaacc gtgcaccgac tctacataga
    18361 ttaggtatac aagcatttga accaatctta gttgaaggta aggctataaa actacatcct
    18421 cttgtatgta cagcttataa tgcagacttt gatggtgacc aaatggcagt ccatgtacct
    18481 ttatcagtag aagcacaagc agaagcaaga ttcttaatgc tttctgtaaa taatatactt
    18541 gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt aggttgctat
    18601 tatctaacaa tagaagccca agatggagct aaaggaacag gtatggtatt taaagacttt
    18661 aatgaactat tacttgctta ttacaataaa tcagttcatc tacatgcatt agtgaaactg
    18721 aaggtaacac ttgaagatgg aagaagttca ttggttgaaa gtactgttgg tagatttata
    18781 tttaatgaaa atatacctca agacttaggt tttgtagata gaaaagaaaa tccatttgca
    18841 cttgaagttg atttcttagc agataaaaaa tctcttggta aaataataga taaatgcttt
    18901 agaaaacacg gaaatacaga aacagctgaa ttactagatt atataaaagc tctaggattt
    18961 aaatattcta cattaggtgg tataacagtt gctgttgatg atatgagtgt cccagaagaa
    19021 aagaaagtat ttatagctga ggcagaagct aaagttgata agtatgaaaa agcatacaga
    19081 agaggtctaa tctctgatga agaaagatat gaaaaagtta tagagacatg gacagaaaca
    19141 actgataaag ttactgatgc tcttatgggt ggactagata gattaaataa tatatatata
    19201 atggcacatt caggagccag aggttctaaa aaccaaatta gacagctggc aggtatgcgt
    19261 ggtcttatgg caaatgcatc tggtaaaaca gttgagatac cagttaaatc taatttccgt
    19321 gaaggtttat cagtactaga atactttaca tcttcacatg gtgccagaaa aggtcttgcc
    19381 gatacagcta tacgtacagc tgaatctgga tatttaacaa gaagacttgt tgatgtaagt
    19441 caagatgtta ttgtaaggga aatagattgt ggaacagaag atactacaga gatttatgct
    19501 ataaaagaag gaaatgaagt tatagaagag atatatgata gaattgtagg aagatatact
    19561 atagacccta tattaaatcc tgaaactgga gaagttatag ttgaggctga ttcaatgata
    19621 caagaagatg aagcagaaac tatagtagct ttagaaattg aaaaaattag gataagaaca
    19681 gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag aaacttggca
    19741 acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca atctatcggt
    19801 gagccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc aggagctgat
    19861 ataactcaag gtctaccaag ggttgaagaa ttatttgaag ctagaaaacc aaaaggatta
    19921 gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa gagaaaagaa
    19981 gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata tggttctaga
    20041 ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca aggatttata
    20101 aatcctcatg atatagtaag agtaaatggt gttaagggag ttcaagaata tatagttaaa
    20161 gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca tatagaagtt
    20221 atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac agatttattg
    20281 ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc tatagataaa
    20341 ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc atctcttgca
    20401 actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt aacagaggca
    20461 gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat attaggtaag
    20521 ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga aaaaattgaa
    20581 gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg ttaaaatagt
    20641 aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ttcttagtga cttttagatg
    20701 gcaataaatg agaactaaaa agcatcaagc tagaaaagtg ctaatatcac atatttatgt
    20761 gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga atattcatat
    20821 tgatgtagtt ggcggtcact gataagatcg aaaatttgga aggaggtgca gatgatgcca
    20881 acaattaacc aattagttcg taaaagtaga aaagcgctag agaagaaatc tactgctcca
    20941 gcattacaaa aaggttacaa ctctttaaat aagaaagtaa ctgacgctag tgccccacaa
    21001 aaaagagggg tttgtacttc agtaaaaaca gttactccta agaagcctaa ctcagcttta
    21061 agaaaagttg ccagagttag attaactaat ggtatagaag tttctgctta tataccagga
    21121 gaaggacaca acttacaaga gcatagtgtt gttcttataa gaggaggaag agtaaaagac
    21181 cttccagggg ttagatacca tatactaaga ggtactctag atacagcagg tgttgataag
    21241 agaagacaat caagatctaa atacggtgcg aagagaccta aagaagctaa aaagtaaaaa
    21301 caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg gcatcacggc
    21361 attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga gggaagcgaa
    21421 atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat gtatgggagt
    21481 aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg aaagtctcaa
    21541 agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga agctttagaa
    21601 gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc aagaagagtt
    21661 ggtggggcaa actatcaagt gccaatcgaa gttagacctg aaagaagaca aacattaggt
    21721 ttaagatggc tagtaaaata tactagagct cgtggagaaa aaggaatggt tgaaaagcta
    21781 gctaaagaga taatggacgc agctaacaac actggagctt cagttaagaa aaaagaagat
    21841 actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta ggatatatag
    21901 atatcttata aaattgagga tatcataagg aggataacca tggctagaaa gtttcctttg
    21961 gaaagaacta ggaatatagg aatcatggct catatagatg caggaaaaac tactactaca
    22021 gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca tgaaggagct
    22081 tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac ttctgccgct
    22141 actactgctt catggaaaga ccatagaata aacataatag atactcctgg acacgtggac
    22201 ttcacagttg aggttgaaag atctctaaga gttcttgacg gttcagttgc tgtattctgt
    22261 gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga gacaagctga aacttatgga
    22321 gtacctagaa tagcttttgt aaataaaatg gatatattag gtgcagattt ctacaatgtt
    22381 gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt accaataggt
    22441 aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt tatatacaaa
    22501 gacgacctag gggttgaaat ggaagaaaca gatataccag aagatatgaa agaattagct
    22561 gctgagtgga gagaaaaatt agttgaatca gtagctgaaa ctgatgaaga attaatgatg
    22621 aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat aagaaaagct
    22681 actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa caaaggtgtt
    22741 cagttagtaa tagatgcagt tttagattac ttaccagcac caactgatat accagctata
    22801 aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga agagccattc
    22861 tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc gttcttcaga
    22921 gtttattctg ggacattaga gtcaggttct tatgttttaa atgcaactaa gaataagaga
    22981 gaaagaattg gtcgtatatt acaaatgcat gctaatacaa gagaagaaat aactaaagtg
    23041 tatgctggag atatagcagc agcagtagga ttaaaagata ctactactgg agacacatta
    23101 tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc agttatatct
    23161 gttgctatag aaccaagttc taaagcagct caagaaaaaa tgggtatagc tcttcaaaaa
    23221 ttagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg acaaactata
    23281 atatctggta tgggtgaatt acacttagag ataatagtag atagattatt aagagaattt
    23341 aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat aactcaacca
    23401 gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata cggtcacgtt
    23461 aagattagag ttgctcctca agagccaggt gaaggataca aattcacaaa caaaactgtt
    23521 ggtggatcag taccaaaaga atatgttggt ccagttgaca tgggtataca aggagctatg
    23581 caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt atatgatggt
    23641 tcttaccatg aggttgactc ttctgagatg gcgtttaaga tggctggttc aatggcattc
    23701 aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa agtagaagtt
    23761 gttactccag aagattacat gggagatgtt atgggtgact taaactcaag aagaggatta
    23821 atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt tccactttca
    23881 gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc aacttacaca
    23941 atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat agctgaagga
    24001 aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag atgagagata
    24061 tctctcatct agcacaagat
//
LOCUS       DAFKMX010000042        24015 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFKMX010000042 DAFKMX010000000
VERSION     DAFKMX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.042"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1170
                     /locus_tag="LZB08_001357"
     CDS             <1..1170
                     /locus_tag="LZB08_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBM7314867.1"
                     /translation="LNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTEGE
                     VYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEGEV
                     DIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVYYQ
                     PKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCNDLS
                     EWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINELN
                     ILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSRNI
                     AKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            1424..2086
                     /locus_tag="LZB08_001358"
     CDS             1424..2086
                     /locus_tag="LZB08_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314868.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            2457..3866
                     /locus_tag="LZB08_001359"
     CDS             2457..3866
                     /locus_tag="LZB08_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBM7314869.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKVSGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVNLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            3981..4256
                     /locus_tag="LZB08_001360"
     CDS             3981..4256
                     /locus_tag="LZB08_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBM7314870.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            4886..5197
                     /locus_tag="LZB08_001361"
     CDS             4886..5197
                     /locus_tag="LZB08_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314871.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            5199..7124
                     /locus_tag="LZB08_001362"
     CDS             5199..7124
                     /locus_tag="LZB08_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBM7314872.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            7127..7618
                     /locus_tag="LZB08_001363"
     CDS             7127..7618
                     /locus_tag="LZB08_001363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBM7314873.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            7670..8233
                     /locus_tag="LZB08_001364"
     CDS             7670..8233
                     /locus_tag="LZB08_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBM7314874.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            8248..9225
                     /locus_tag="LZB08_001365"
     CDS             8248..9225
                     /locus_tag="LZB08_001365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBM7314875.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            9212..9535
                     /locus_tag="LZB08_001366"
     CDS             9212..9535
                     /locus_tag="LZB08_001366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBM7314876.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            9652..11430
                     /locus_tag="LZB08_001367"
     CDS             9652..11430
                     /locus_tag="LZB08_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBM7314877.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            11427..12800
                     /locus_tag="LZB08_001368"
     CDS             11427..12800
                     /locus_tag="LZB08_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBM7314878.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            12806..13474
                     /locus_tag="LZB08_001369"
     CDS             12806..13474
                     /locus_tag="LZB08_001369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBM7314879.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            13751..14800
                     /locus_tag="LZB08_001370"
     CDS             13751..14800
                     /locus_tag="LZB08_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7314880.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            14896..15885
                     /locus_tag="LZB08_001371"
     CDS             14896..15885
                     /locus_tag="LZB08_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7314881.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLESSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            15890..17608
                     /locus_tag="LZB08_001372"
     CDS             15890..17608
                     /locus_tag="LZB08_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBM7314882.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLIGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDNILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            18050..18781
                     /locus_tag="LZB08_001373"
     CDS             18050..18781
                     /locus_tag="LZB08_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBM7314883.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYKLIEEYSCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYRANTQSEFLLGGTESFKESAIRNNKEYKTKVV
                     HFEEIIIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMENELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFQQSQRINNIK"
     gene            complement(18797..20536)
                     /locus_tag="LZB08_001374"
     CDS             complement(18797..20536)
                     /locus_tag="LZB08_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314884.1"
                     /translation="MNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKQNMDVLRII
                     PTVNNIPVDYEFEADYFSILIKFNNGSISICFDGDSKILFYGKGKNVGLKLDTLPVYN
                     FEYNYLMGKKDSEYCVINSYKNLTKFLVFSVDGKVNLSQNIFIDDCASTNKANNTSII
                     DVNNNTDNEFTCIIEDIPTHMKVPDYKEYNFETSLNNTKKLFNEYYLAQPKIDEKYKN
                     SLLRASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVDDKLAFDQ
                     MAVLFDYQDEYGQIPGSLSDSTIRWNFTKPPIQGLFFNKMMKKINFTKETLEEIYNYI
                     EKQVLFYIKFKDSNMDGIFEYHHGNDSGQDNSTIFKGVMVVDTPDLTAFIINAMDMLA
                     KVAEKLNKYSEKEYWANKADELTKLFLSYFIVDDLPVARETLTGKILPNNALLPLVSL
                     VLGNRLPKYTRDKMIDLIISERYLTKWGIATEAIDSDEYQDDAYWRGPIWAPTTLLFV
                     EALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTFIYLAAK
                     VYEYNTQRINNIK"
     gene            20784..22217
                     /gene="treB"
                     /locus_tag="LZB08_001375"
     CDS             20784..22217
                     /gene="treB"
                     /locus_tag="LZB08_001375"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBM7314885.1"
                     /translation="MGKNKILNSKESISQIIEYVGGKENIQSATHCVTRLRLILKDTN
                     KANINKLEGIDIVKGCFNAGGQFQIVIGVGVDKVYKDLIEQTGAEEVSKEEIKQQVNE
                     KSNKLQKFIRVLADIFIPILPAIVASGLLMGVNNMIANPGIFYEQSVIEVNTGIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILTKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVHLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            22280..23938
                     /gene="treC"
                     /locus_tag="LZB08_001376"
     CDS             22280..23938
                     /gene="treC"
                     /locus_tag="LZB08_001376"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBM7314886.1"
                     /translation="MLDFKKSVVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKEAKKRNMGIMLDIVFNHT
                     STEHEWFKKALKGDKYYKDFYIFKNPKEGKSAPTNWESKFGGNAWEFVEKFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQGDGRH
                     FYTDGRKVHQYLKEMCEESGVLENDMLTVGEMSSTTIENCIKYSNPNEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKLLNEWQIGMEKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYVYQGEEIGMTNAYFTNIKQYVDVESLNYFDILKS
                     QGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKPWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIELRKKYDVVAYGDFKPLLEENDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVILDNEELEKYKCIISNYGDRKVTSTITLRPYECMALFKNEK"
BASE COUNT         9252 a         2852 c         4287 g         7624 t
ORIGIN      
        1 ttaaattata ttatagtata ttttgatatt aataacttta agatgattaa tgatactttt
       61 ggatatgaat ttggtgacaa tttattaatt acaatagcaa aagctttaaa agaagaatta
      121 acagaaggtg aggtatatgc acgactttca agtgattatt ttgcaatttt ctgtgattat
      181 aaaaatggaa gaaataaaat tataagaaaa cttgataata tacgaagtaa tattgaaagt
      241 aatttgagta tagtctttga aatctctctg tgtgtgggta tttattttgt tgaagaaggt
      301 gaagtagata ttcaaaaggc tgtaaataaa gcaaatatgg ctaggtctgt tgctaaaggt
      361 aaaaatataa attatgctat ttataatgaa gatgttagga ataagcttag tgaagaatca
      421 atgattttag atgatataaa aatagcattg gtaaaaaatc aatttgaagt gtattatcaa
      481 cctaagtttt cacttgtaac tggagaaatg atagggagtg aagcactaat aagatggaat
      541 catccagaac atggttttat aagtcccgca gtatttattc caatagcaga gaaaagtaag
      601 ttgattttaa aaattgggag atttgtattt gaaagggttt gcaatgattt atctgaatgg
      661 aaaaagcaag gtaaaaagat agttccagtt tcagtaaatt tatctagagt cgagttgtat
      721 caaccagata ttgtaaaatt tatcaataaa acaatacaaa tgtataatct aagttcagat
      781 ttcattgaaa tagagataac agaaactgtg gctattaatg agttaaatat tttaaagaat
      841 gttttaaatg aattgaggaa gcatggattt tctatttcta tggatgactt tggcacagga
      901 tattcttcta ttagttgttt aagagatatg ccaattgata tattgaaact agataagtct
      961 tttcttggtg gcattgaaca tgatgagaga agtcgcaata tagcaaaatc aatagtgtct
     1021 ctagcaaaat cactagattt agttgtaatt atagaaggtg ttgagagtaa agaacaagct
     1081 gagttaatga aacaatttgg atgtgattta gttcaaggat tttattttgc acgaccaatg
     1141 ccagctaaga attttttaga tttactttaa aacaaaattt gatttttata taaagcacta
     1201 ttatgaatgc ttgattttaa taaatcattg ctaaataatt agtgtttttt tattttatca
     1261 atgtattgtc taatataaag caaaaaataa ttgtatttta taaaaattat attaaaaata
     1321 aatttatttt gctaataaac atgttattaa caaaagaata gtactataat gtatagatga
     1381 taaaaacttt aataaaaatt tagtgtatag gagttgttta agtatgatag atgtaagtat
     1441 tgatgacaaa cttaaggaaa gatggcctaa tgctaagctg ggttgcattc aagcaaaagt
     1501 agcagtaaat aaaagctcag aaaaattaat taatgaaata aatgagtttt gtgatactat
     1561 aaatcaaagt ctaaaagttg aagacattac gaaactagat aaaattaaag atgcaagaaa
     1621 tgcttataaa gagcttggaa aaaatccaag tagatataga acatcttcgg aagcattggt
     1681 tagaagaatt attcaaggga agggtcttta tacaataaat aatatagtag aaataaacaa
     1741 tttaatatca ataaaatcat tatatccagt aggtacatat aatgtaagca aattacattc
     1801 accagtatgt tttactgtag gagatgaagg agaacagtat aagggcattg ggaaagaatt
     1861 aataaatata gaaaatcttc ctatattgtc tgattcagtg ggtaaatttg ggagtccaac
     1921 tagtgattct gaaagagcaa tgataacaga agatgcaaat gaaatattaa tttgtatatt
     1981 ttcttttagt caagaatctg atattgaaga atattttgga tatgctaagg aattacttaa
     2041 agaatatgca gatggaaaag atatagaaac taaaataatt aaatagaatc tatataacaa
     2101 cgttatgtta agctaaaatt caatataaaa ataagttaag ataaagaaac taacacaaaa
     2161 ttagtttctt ttttaaaaga gtagtttagt gttacgaaaa tagatagata gtcccacgat
     2221 tattaaaagt atggtaatat attaatatag cagagattgt gtttaaggga ttttgaaagg
     2281 ggggatttat aataatcatt ttatctaaaa gataaaatgg ttttatggaa ataactatat
     2341 atttaaaaat ataaataatg atacagtaat ttgggtatat aaaataaggc atacatggtt
     2401 ttgtttaaaa accaataagg tacataggta attacaatgt aaggaaggat ttaattatga
     2461 ttgatggaaa agaggtaaaa caagaaagtg gacaaaaaaa tctaaaattt aagatagcta
     2521 tagtagtagg tgttttgttt gtagcttata taggaatggc aatctttttt agaaatcatt
     2581 tttactttgg aacgactatt aatggcgtaa aggtttcagg aaaaacagtt gagcaagtaa
     2641 atgatttgtt aacatctagt acaaattcct atactttaaa cttagaggaa agaaacaata
     2701 agaaagaaca aataaaagca aaagatattg atttaaaatt tacaccagat gataaagttc
     2761 aaaagataaa agatagtcag agcgctattg gatggattgg tggaatattt aagtctaaag
     2821 agtataataa tatggtaaat ctttcatata ataaagattt attagaaaaa agatttaacg
     2881 ctctatcttg ctttgatagt aaaaatgttg tagaacctaa gagtgcttat cctaagtatg
     2941 atagcactaa aaacgcttat gttatagtac cagaggtact tggaaacaaa gtgaaaaaag
     3001 atgattttta taagttagta caaaactcaa ttaattctgg gaatacttct atttctctgg
     3061 atgaaagtaa agtttatgaa gcaccaaaca acacaaagga ttcagaaaag ttgaaacaag
     3121 ctattaagac attagataag tatgttggtg tagtcgttac atatgatttt agtgaccgta
     3181 aggaaacttt agatggttct gaaattaata aatggttaaa agtagactca gaaaagaatt
     3241 atgacataac ttttgataat gatgctatgg tagaatatac aagaggttta agtaaaaaat
     3301 acagtacttt tggtgactca agaccattta aaactgccac aggtaaagat attacaattt
     3361 caggtggaat ttatggttgg ttgatagata aaaagaaaga agctgaagca ttagttgatg
     3421 ttgtaaaagc tggtaaaaat gttacaagag aacctattta catacaaaaa gctgtatctc
     3481 gtaataaaga tgatattgga aatacatatg tagagattag tctttctggc caacatatgt
     3541 ggttctttaa aaatggagaa atgcttgtat caacagacgt tgtaacaggt gatttagcta
     3601 gaggatttgc tacaccatca ggtatatatc ctcttaatta taaggctaga aatgtttcat
     3661 taactggtca aggttacagt tcacctgtaa gttattggct tccatttaat gggaacatag
     3721 gcattcatga tgctacttgg agaaattctt ttggtggaag tatatataag tcttcaggtt
     3781 cacatggatg tgtaaataca cctttttcta aggcaaagaa aatatatgaa aatatagaac
     3841 caggtacacc aattatttta tactagtaaa tgtgttgcta acagttaagc gaagtcatct
     3901 tagtctattt aggatggctt cctttttata taaagcttat tgaagtttac agaaagacta
     3961 cttaaaagga gaaatacata atgaatgaaa aattaactaa aattttagaa gaagctaaga
     4021 atgatgatga atttagaaag gaacttataa aaacaagaga acaacgtgat gttatggatt
     4081 cattttgtaa ggttgttaca tctagaggtt atgatatgac agtaggggaa cttttttcta
     4141 tgggagaaga atatacatcc aatctactga aaagtgtaaa tggaggagca gcatatccta
     4201 ttgaagattg gagtgattgg tacgaggatt tttttataat gttgtcgaat atatagagta
     4261 actaatgata ttatctactt aagattaata cattgcctta aaataaaact attagaacta
     4321 ggtgtaagtt ttatcaatct ttaaaaatat tgatttattt ctaactagat ataaaaaaat
     4381 tagattaaaa ataaggtgac aaaaatcgat atagcttgat gatttataaa agttagataa
     4441 aatagtgcga gatatttcta aaaattcaaa aaaaacaata aacttcaatg gttagaggtt
     4501 gaaaaagaat ataaaaaact tttatatgta ataaattaaa ttaaaattat aaaaaaaata
     4561 aaataaaaat ttaaaatgtc gacataacgt taattttaaa acaaaataac gctattgaca
     4621 aactatatcg ctttaaatta taattattga aaataatggt atttaaaaac catactcatg
     4681 tcatagtcta aaagtaagtc aaaaaaggat gtaaatctag gcaagtacta ttctaaaata
     4741 tgttttatta agaaataaac gttgaaaaat attaattatt atataaaaat gctgtttata
     4801 ctttaacaat gaggtagtaa tctaattaga ataaaaaaat ctaattggtt atatacatag
     4861 taaaaaggta agagaggtga tttggatggc ttttgaaata ataaaaagca ttgttgaggc
     4921 agagcagaca gcagacagta tcaaagtaaa agctgttact gatgcagagt ctatcagagc
     4981 tgatgctgta aacaaatgtg aaagcatatt tgctgatgta aaaaaacaag caaagcttat
     5041 ggaagaaact cttattgaga aggcagtcac cgacagtaga gcagaggttg ataaaatctt
     5101 agctaatgct aaaagtgaat gtctgaaaat tgaaaaaact gctgaagaaa gaaaaagtaa
     5161 ggctattgaa gcggttattg gaaaggtagt gagataatat ggcaatagtt aatatgaaca
     5221 aaatatctat tgttggctta gaagctcaaa aaagccagat tttaaagcta cttatgaacc
     5281 gtgggtttgt tcagattgat gatagtgcct ttcttactga ggaggaggaa ttaaaaaatt
     5341 atcttgtaaa agatagtgaa gaatctgaag ttataatgct agagcagaaa atgtctcatg
     5401 taaaccaagc aattaatatt gtttcttcac tagtgaaaca aaaaaaatcc ctacttgcac
     5461 caaaaagaga gtttaacaaa attgatagtg aaaaagaagg gcaagtttat gctgaagctc
     5521 tggagttaaa caatttgcac aaggagatag gtacaataag gtctaacata aacttactta
     5581 acaacaataa aagtatgtta gaaccatggg tgaactttga tattcctctt gaaaatatgg
     5641 aaactaagta tacaaaaact atacttggaa ctgtgcccat aagcgcaaac attgatgctt
     5701 taagagtaaa attagaagat gaaataccag aaagtgttat tgggattgtc aattctgaca
     5761 agttaatgaa ctatgtttat ttaattactt taaaagaaac ttttgatgat gttttagaag
     5821 ttttaaaaga atttaatttt tcagtggttt cattaccaaa tgaagaaggt acaccgttac
     5881 aagctatcaa gaaatacaat gaaatgataa ttagacagga aaacgtttct aaggacaaaa
     5941 cagaggaaat taaaagacat gcagatagta tacacatgct tgaaaaccta tatgattttt
     6001 acactataga aagagaccaa aaaaagatta tagagagact tgtcaaaaca aaaacaacat
     6061 tctgtcttaa tggttggtta ccaagcaaaa gggcagatga attaattaaa gaaattacag
     6121 aaaagtttga ttgttgtgtt cagactgaag aaggaagtaa agaagaaggc ttcccaattc
     6181 tgcttgaaaa caataaatta gtaacacctt ttgaaagtgt aactaacatg tacagctatc
     6241 caagtacaaa agatattgac ccaaatacaa ttttaacttt cttctttgtt gtattctttg
     6301 gaatgatgct aagtgacgcc gcatatgggt taataattgc agtagtttgt ggatttgttg
     6361 tatataaatt aaagatacaa aaaggtgaag gaaatttaat taaacttatt ggtatctgtg
     6421 gagtttcaac tactgtatgg ggtcttatat tcgggggtat tttgggggat ttaattccta
     6481 taaaagcatt aattaatcct ttagaggatg ttatgacact tatgggaatg tcgttactgt
     6541 tcggtattat ccatatatat gtaggtttgg gaataaaagc atatacatta ataagaagtg
     6601 gaaaggtaat agatgcaatc tttgacatag gtttttggta tttgtgtata acaggtgtat
     6661 gtctacttat aatacctttt gttgcagggg acataggtgt attttcagaa gtaggaaaat
     6721 atttagcaat tataggtgct ataggtcttg tacttactca aggaagaagc tttaaaggta
     6781 ttcctgttaa gctttttaaa ggattttcaa gtctctatgg cataacaagt tattttgctg
     6841 atatattatc ttatacaaga attatggcgc tttgtttaac aacaggagtt atagcacaag
     6901 ttatcaacct tttaggagct atagctggtc cgatacttgc agttgtaatt ggtgttgtag
     6961 gacacacaat aaaccttctt ataaatgcac taggtgcata tgttcataca agtaggttac
     7021 agtatgtaga atttttcaat aagttttatg aaggcggagg agttcctttt gttccgttta
     7081 agtataaaac aaaatataca agcattaata aaaaggagat gtaaatatgg aatttttaaa
     7141 cgcgttagga aatggtcaat ttttggcaat atcaggagct gctatagcag cattatttgc
     7201 aggtatgggt tctgctaaag gtacttcaat agcaggtcaa gccgcagcag gtgttgtaac
     7261 agaagaccct agtaaattcg gtcaacttct agttttacaa cttcttcctg gtacacaagg
     7321 tctgtatgga tttatcatag ctttccttat tttatcaaaa gcagggatta ttggtggaga
     7381 tgcaatacca acatcagctc aaggtttaca acttcttatg gcaggacttc caattggttt
     7441 agttggttta gtatcaggta tagcacaagg taaagcagca gcagcaggag ttggaatagt
     7501 tgcaaaaaga cctgaagaac ttggtaaagc tataacattt gcagcaatag ttgaaacata
     7561 tgctctttta ggactacttg tttctttctt agcttacaat ggaattgcaa ttggttaatt
     7621 ataaattata attcataagt aaaaaaaatg aaaaggagga agcactccta tgggtaatga
     7681 acaaaaaatg attgacagaa ttatagctga tgccaaacaa gaagcacaag aaatattgga
     7741 taaagctaaa agtgaagcag atttaaaagt aaattcagct aatgaaaaag ctgaaaagga
     7801 aatggcttcc tatacaaagc ttgcagaggc agaagcagaa aaagcggctt ctaaggaaat
     7861 atcaggagct tatatggagg ctaaaaagca aatattatca aaaaaacaag agattttaga
     7921 agaggtaatt ttagaagcta aaaacaaact tttaaatctt aaagataatg aatatgaaga
     7981 gattatacta aacatgattg aaaaatcaaa ttgtacagat gattcagaga tagtattatc
     8041 taaaaaagat aaaaaaacat taaaagatgt gctatcaaaa aaaggcatta aagtgtctga
     8101 tgaaactaga gatattactg gaggtttcat agttaaaaaa ggtgacattg agtacaatta
     8161 ttcatttgaa gccataattg cagtagagca tgaatatatt gaacaaattg cagcagaaat
     8221 tttgtttaat taaggagggg ttaaaaaatg gcagaagaaa aaacttatcc ctatgccgtt
     8281 gcacgaataa gagtgcttga aaaacagctt cttaacagac aaatgtttat tcaaatggca
     8341 gaagccaaaa gccctgaaga ctctttaaga attatagctg aggcaggtta tgctgataca
     8401 tcaaataata atatacataa ttttgagaat attttgactc aagaattatc aaagacatat
     8461 gaaattctta aaagtctagc tcctgaagaa aaatttgttg atgtatttct ttataaaaat
     8521 gactatcaca atttaaaagt gctgattaaa gaagaaattt ctggtgtaga tggtgagaaa
     8581 tatttaattg atggaggtac aattcctctt attaagctta gagaatcttt agcaaatcgt
     8641 agttttagtg atttacctaa gataatgtca tcagctatat tagaggcatt tgatgtttat
     8701 aataagacac aaaatggcca gatggttgat attgttctag ataaagcaac ctttaccagt
     8761 atgaaagaaa ctgctaaaga aagccaaaac aaatttgtaa tagattatgt tatgaaagtt
     8821 tgtgatttaa caaatctaaa gagttttata agagtcaaaa atatgaagaa agattttgat
     8881 atgtttatga atgtgtttgt ttcaggtggc tcacttgata aggaaaaatt cttagaagct
     8941 ttttcctcag atactccagc atcatgtttt aaatctactt cttactctga tgtctgtaaa
     9001 aatggcatgg atagtggttt tactgtcttt gaaaaacttt gtgatgacta tcttatggaa
     9061 tatgttagag gagcaaaatt taagcctctt acattagaac cactaattgc atatttatat
     9121 gctaaagaat cagagataaa aacaattaga attattttga ctagtaagct taataatata
     9181 gacgcagata ctataaaaga aaggctaagg gatgcttatg tataaggtag gagtagttgg
     9241 agataaagat agcatcatgg gatttttagc tcttggaata gatatcttcc cagcctatga
     9301 tagtgacgaa attaaaaaaa gtatacataa gttggttgaa gatgaatatg cgataattta
     9361 tattacagaa caggcatcat tgcttgcaaa agaatcaata gcaaaataca aagattacca
     9421 acttcctgcc attatagtta ttccaggtat tggaggaagt atgggattag gtatgaatga
     9481 agtaagagaa tctagtaaac gtgctatagg tgcagatata ttatttaaag aatagatgaa
     9541 agttgaataa aagacaatct caaattttat tggtgctaaa tatatgtact aataaaatat
     9601 aggagggaat atttaaattt tgataaagta tatgattgca ggtgagagaa tatgaaaaca
     9661 ggcacaattg taaaggtgtc aggaccgctt gttgtagcgg agggcatgag agatgcgaat
     9721 atgtttgacg tcgtaagagt ttcagataaa catcttatag gcgagattat agaaatgcat
     9781 ggagataaag cttctataca ggtttatgag gaaacttccg gtctaggacc aggggaagaa
     9841 gtagtttctc ttggaatgcc tatgagtgtt gaacttggac caggtcttat ttcaactatt
     9901 tatgatggta ttcaacgtcc acttgaaaaa atgtatgata tttcaggtac taatataact
     9961 aaaggtgttg aagtatcttc acttgataga acaactaagt gggaatttaa acctaaaaaa
    10021 tctgttggtg ataaggttgt agcaggagat ataattggtg gcgttcaaga aacagcagta
    10081 gtagaatgta aaattatggt tccttatgga gttaagggta ctataaaaga aatatattct
    10141 ggtgaattta ctgttgaaga tacagtttgt gttattacag atgaaaaagg taatgatata
    10201 cctgtaacta tgatgcaaaa atggccagtt agaaaagaaa gaccatatag agaaaagaaa
    10261 acaccagatt cacttcttgt tacaggtcaa agagttatag atacattttt cccaatcaca
    10321 aaaggtgggg tagcagctat accgggacct tttggaagtg gaaaaactgt tacacagcat
    10381 cagcttgcta aatgggcaga tgcagatata gttgtatata taggctgtgg cgagcgtgga
    10441 aatgaaatga cagatgttct tcttgaattt cctgaattaa aagacccaag aacaggcgag
    10501 tcacttatgc aaagaactgt gcttatagca aatacatcag atatgccggt tgctgcacgt
    10561 gaagcttcta tatacactgg tattacaata gctgaatatt ttagagatat gggatatagt
    10621 gttgcactta tggcagactc tacatcaaga tgggctgagg ctcttagaga gatgagtggt
    10681 cgtttagagg agatgcctgg tgaagaaggt tatcctgcat acttaggttc acgtcttgct
    10741 caattctatg agagagcagg aaaggtaaat tgtctaggta tggatgaaag agaaggaaca
    10801 cttacagcaa ttggtgcagt ttctcctcct ggtggtgata catcagaacc tgtcagccaa
    10861 gcaacgcttc gtatagtaaa agtattctgg ggtcttgatg catctttagc atataaacgt
    10921 catttcccag caattaactg gttgactagt tattcactct atcaggaaaa agttgatgta
    10981 tgggcagata aaaatgtaaa tgataatttc tcagctcaaa gagctcaagc tatgaaactt
    11041 ttacaagttg aatctgaact tcaagaaata gttcaattgg ttggtgttga ttcactttca
    11101 gatggagata gattgatact agaggttact cgttctataa gagaagactt ccttcatcaa
    11161 aactctttcc atgaagttga tacttataca tcacttcata aacaatatag aatgatggga
    11221 cttatattaa aattctataa gtcagcacaa gatgctatca agcaaaatgt tactataaac
    11281 gatattatca agctttcagt tcttgaaaag attggtcgtg ctaagtatgt agaagaaggc
    11341 gatatagatg cagcttacga ttcaattgaa gatgaaatag acaacgagct tgcagacctt
    11401 attaaaaaga ggggggctga agaactatga taaaggaata taagtcaata caagaggtag
    11461 ccggtcctct tatgttaata aacggcgtag aaggtgtaaa atttgatgaa cttggagaaa
    11521 ttgaactttc aaatggagaa attcgacgtt gcaaagttct agaggtaaat ggagatactg
    11581 cacttgttca gcttttcgaa agctcaacag gtataaacct tgctgaaagt aaagttcgtt
    11641 ttttaggaaa gagtcttgac tttggagttt caccagatat attaggtcgt gtttttagtg
    11701 gaatgggtag acctattgat ggaggtccag aaattatacc agataaaaga cttgatataa
    11761 atggagctcc aataaatcca gcagcacgtg attatccggc cgagtttata caaactggag
    11821 tctctgctat agatggactt aatacattgg ttagagggca aaaacttcct atattctcag
    11881 ggtctggttt gccacatgcc aatcttgcag ttcagattgc aagacaggca aaagtacgtg
    11941 gaactgattc taagttcgcc gttgtatttg ctgcagttgg tataactttt gaagatgctg
    12001 aattttttat aagcgatttt aaagcaactg gtgcaatcga ccgttctgta gtgtttgtaa
    12061 accttgcaaa tgacccagct gtagagcgtg tatctacacc tagaatggca cttacagcag
    12121 cagaatacct tgctttcgag caagatatgc atgtacttgt tataataacg gatattacaa
    12181 actatgcaga ggcattgcgt gaagtttcag cagctaaaaa agaagttcca ggacgtcgtg
    12241 gatatccagg atatctttat actgaccttg ctactatgta tgaaagagca ggaaaaatga
    12301 aagggcatga agggtctata actatgattc caatacttac aatgcctgag gatgataaga
    12361 cacatccaat tcctgacctt acaggataca ttacagaagg gcaaattata cttagccgtg
    12421 aactttataa aaaagatata cagcctccta tagatgtttt accatcactt tctcgtctaa
    12481 aagataaagg tattggtaaa ggtaagactc gtgaagacca tgcagataca atgaaccagc
    12541 tttttgcagc ttattcaaga ggtaaggatg caaaagaact tatggctata cttggtgaat
    12601 ctgcactttc tgagattgat ttaatgtatg ctaagtttgc agatgaattt gagaaagaat
    12661 atgtttcaca aggatttaga actgatagaa ctattgagca gacattagaa atcggatgga
    12721 agcttcttag tatgttacca aaaagtgaac ttaaacgtat ccgtgatgaa tattttgaaa
    12781 aatatatgcc taaggagtga tatctgtggc tagattaaat ataaatccaa cacgtatgga
    12841 aatgactaga cttaagaaac tccttaaaac tgctacaaga ggtcataagc ttcttaaaga
    12901 taaacttgat gaacttatga aacaatttct tgagattgta agagaaaata agcgattgcg
    12961 tgaagaagca gaaaatgctc ttgatactgc ttataaaaac tttataatcg caagagcagt
    13021 tatgagtcag gagtatctgg gttcagcact tatgatgcca aagcaatcag tttcagtaga
    13081 tgtttctacg agaaatataa tgagtgttga tgttcctgtg tttgacttta aaactgaaaa
    13141 caatcaaagt gatatttatc cttatggtct tgcttttaca tcaggtgaat tagattctgc
    13201 tatggaagct ttctcagatg ctatgcaacc acttttaaga cttgcagaaa gtgaaaaaag
    13261 tgcacagctt cttgcacaag agatagaaaa gacacgtaga cgtgttaatg ctcttgaaaa
    13321 tgtaatgata ccaaattaca ttgagactat taagtatata gctatgaagc ttgaagaaaa
    13381 tgagagagca agtactacta gacttatgaa ggttaaggat atggtgctta aaaaagcact
    13441 tgaagaaaag aaaaaaaatg atttggttgt ataattaaat acttataaaa ataagtctaa
    13501 cattctactt tatagaggtt agacttattt tttatgttta aattaaaatg tagatactat
    13561 aaaaattaat atattgttta aaaacagact gtatttaact aaaaataata tatttaacaa
    13621 aaatttctaa aaattaacga actgttaatt tacacttaac tttatattaa caaattaggt
    13681 gtattatata aacataagca aaaacaataa aaaacaaata aaaaaaggaa aaaacacaaa
    13741 gggggaagat atgaaagaat ttatatttac ttgtctgtgc attgtagtga ttacatttat
    13801 gacaagttgc atatcaacaa caccacaaac tactcaagga aaaggaaata ataaacttac
    13861 aatatatgca gcactagagg aagagcagat agcttattat atggaaggtt ttagagaaga
    13921 gtatcccaat atagatgtag agatagttat ggattctcat ggtgtaatag cttcaaaact
    13981 attagcagaa aaagataatc cacaagcaga tattatatgg ggattatcag caatgaacat
    14041 gatagagcta aaaaaagatg gtgcacttga agcatataag cctaaaaatt atgataaagt
    14101 tgataaaaaa tttaaagata ctgaagatga tgtatcttgg gtaggaatga gtgtcccaga
    14161 gacagcaata gtagttaatt ataaggaact taagaaacta ggtatagata ttccaaaaag
    14221 ttatgaagat ttaataaaac cagaatataa aggacttata acaatgccaa atccagcatc
    14281 atctggaacg gggtatctta cagtatcagg gcttattcaa atgatgggtg aaaaagatgc
    14341 ttacaattac atggataaat tacataaaaa tatagccaca tatacacact cgggttcaaa
    14401 acctgcgaag ttagctggct ctggagagta tccaataggt attacacttg gatatagagc
    14461 tacacaacaa ttatctagtg gagaacctat agaggttgta tttccaaagg aaggttcagg
    14521 ttgggactta gaagcaaatg ccttagttaa aaaagataat ataaaaaaag aagcaaagct
    14581 atttttagat tgggcaataa gtgattcagc tatggataga tatgcacatg aagtagcagt
    14641 tacttccata aagacagata atccagttcc aaaaggttat tctaaacaac catttgaaca
    14701 gcttataaaa gaagttgatt taaactctgc tgccaaaaac agaagtacag tacttaaaac
    14761 ttgggaatca aaatacggtt caaaatctga aagtaagtaa agtttaaatt aataatttag
    14821 atgaaagaat tttagaagaa aaagtaggat ataaaattta aaataaaaaa ataactatat
    14881 aaggagagat aagatatgag ttacttaaaa ataaataatg tgtttaaaag ttatgaccaa
    14941 aaaagggttt taaataatat tagtttggat attgaagaag gggaatttct atgtttactc
    15001 ggacctagtg gatgtggtaa aactacatta cttagaataa tagcaggtct tgaagatgta
    15061 aatagtggaa ctataattct ccaggataaa gatattacaa atttagagtc atcaaagaga
    15121 ggttttggta tagtatttca atcatatgca ttatttccga atatgactgc ttataacaat
    15181 atagcttttc cactaaaaga gagaaaagtt tcaaaagaaa aaatagataa taaagttaag
    15241 gaagtacttg aaacagttgg attgactaat gaagctcata aataccctaa agcattatca
    15301 ggtggacaac aacagaggat tgcaatagca agagcattag ctcttgaacc aaagttttta
    15361 cttttagatg aaccaatgtc cgcattagat gcaaaggtta gacacaagct tagaatggac
    15421 attaaaagat tacaaaaaga acttaatata acgacaataa tggtaacaca tgaccaagaa
    15481 gaagctataa ctatggcaga taaaatagct attctaaatg gaggagatat aatgcaaata
    15541 gggactccag aagaaatata ccaaaatcct caaaacctat ttacagctca atttatagga
    15601 gatacaaact gttttgataa tggagattct atattaactg taagaccaga atatgttcaa
    15661 atagagaaat cgacaaaaga aaactaccaa gggataatat caaatataga gtttagaggg
    15721 aatttattga gagttgaaat aaaagataaa ttaaatgaaa atttcataat aagtgatgtt
    15781 tctataaaag agtgggttaa tttaaattta gtagaaggtg accttgtaaa aataagtata
    15841 gacgaaaaat actatttaaa gtatccaaaa gttaaaggag cataagtata tgaataataa
    15901 tgctataagt tctaaaagta taagtcttaa atcaaaaatg aatttagata taaaaaaatg
    15961 tatgcttaca gtattagtta tgtctttagt aatattttta ttagcaccta taatatatct
    16021 atttgtacag gcttttttag ataaagatgg agtctttgta ggattacaaa actttcaaga
    16081 atacttggct tctgaatctc tacttaattc tctaaaaaat acagtatttg tatcagttat
    16141 atcaactgta atatcactaa cacttgcatt agtatatgca tatgcattaa ctagaacaaa
    16201 gattaaattt aaaggttttt ttaaatcatt agctatgttt cctatgtttg ctccaacaat
    16261 gatgcatgca ataggtctag tatatttatt tggaaatcaa ggtttggtaa ctaatttaat
    16321 tggagtgcat gttccaatat atggagcagt gggaataata ttggcacaaa ttatatatgt
    16381 atttcctcaa tcatttcaaa tactttatat atctctaagt tcaaccgatt atagactata
    16441 tgaagctagt aatactatgg ggataagcaa gataagagaa tttataagca taacaatccc
    16501 aagtataaaa tactctttaa taagttctgc attcgtatcg tttacattat gttttacaga
    16561 ttttggagct ccaaaggtga taggaggaga gtttaatgtg ttatcaatag atgtatataa
    16621 gcaaattata gggcaacaga atatgccaat gggagcaact gtatcaatat tacttttaat
    16681 acctgcaata atatcgttta tagtaactaa aaaaattgaa aaaaatcaaa attcatatat
    16741 aacttcaaag tcaattccat ataaggttaa aactaataag ttaagagata atattttagg
    16801 aggggttgta ggaattattg catttggtat aatagctgta atgttaactg taatcttatc
    16861 ttcattaata tcagtttggc catatgattt aagactgaca ctggaacatt tttctatgaa
    16921 aaacagtatt gatgggatta aaccatttat aaattcaata aaaatgtcac tattgacagc
    16981 tattctaggg acgagctttg ttttcttatt tgcatatttg aatgaaaaaa cagaaaaagc
    17041 tccatattta aaatcatttg ggtatttttt aagtacattg cctatggcat tgccaggatt
    17101 agttttaggt atagcttatg tattattttt taataaatta gagtttaatt ttatggatac
    17161 aatatacatt aagaatttgt ttaatggatt atatggaaca ttacttataa tggttatatg
    17221 taatatagtt catttctttt cagtagcatt tataacagct actactgcta ttaagaaagt
    17281 agataaagaa ttggatatgg tagctaaatc aatgggaata aacagtgcat caattcttaa
    17341 gaacgtaact ataccacttt gtttgccagc agttttagag aactttatgt actttttcct
    17401 aaactccatg gtgacaatat cagcactagt ttttatatat actgcaagtt caaaagtagc
    17461 tgcaatatca attgtacaac tagatgataa ggggagtact gcacaggcag cagcactagc
    17521 tgtgttaata ttagtcacaa atatattagt gaaagtaata tatgaattta taaagaatcg
    17581 tttagaaaaa agaacaaact ttaattaaat aatttatctt ataaaaacac aaatttaaaa
    17641 aagtctattc ttgtgattta ttatttaaag aatagacttt tttaaatttt aattaatatt
    17701 aatagtgtta attttccttt tttattatac atttgaaaat atgcataatt atatttatca
    17761 aataaagaga tgtctatgga aactttctct aaatctaaag tttttggtgt tactggaaga
    17821 ttggttggat ttagaatatt atcaagctaa gtttgacaaa ttattaagtt tcgcataaaa
    17881 ataatgtttt aatagttttt acagttgaag ttttttataa aaagtttagt tccagtttta
    17941 aatttatatt tattttggtt tttgcttaac ttataatata ttatattaat acatgatttt
    18001 tagaaataag acacaattaa atatttatat ataaaattgg gaggttaaaa tgggaagagc
    18061 caaatattat gatatatata tagatttaaa aaatagaata gaaaataagg aatatttata
    18121 tcaagattta ttaccatcag aatacaaatt aatagaagag tatagttgtt ccagaaatac
    18181 aattagaagg gctatacagg aattagcaaa ccaaggatat gttcaaagta agcatggaaa
    18241 aggagtattt ataatttata gggctaatac acaatcagaa tttttattag gaggaacaga
    18301 atcatttaaa gaatccgcta ttagaaacaa taaagaatat aaaacgaaag tagttcattt
    18361 tgaggaaatt attatagatg ataaaaaaag tcaaataact aactttgaag ttggaacaga
    18421 agtgtattat atacaaagag taagaatatt agaaaataaa ccatctatta tagatcataa
    18481 ttattttatg aaggatatag taaaagatat taatgaagaa atagcatcta attctatata
    18541 tgaatatatg gaaaatgaat tgaatgaaac tatagtaaca acaaaaagaa ccatgacagt
    18601 tgaaaaagca actaaactag ataaaaaata tttggaatta ggagattata attgtgttgc
    18661 agttgttagc aataatactt ataatgctca aggaataatg tttgaatata cacagtctag
    18721 acatatacct gatggattca tatttttcca acaatcccaa agaattaata atataaaata
    18781 gagtacaatt tgtactctat tttatattat taattctttg ggtgttatac tcataaactt
    18841 tagcagctaa gtatatgaat gtactagctg tccaggtaaa agatttatct ctaagacctt
    18901 ctcctgtaat agcattgaaa ttttcagcaa atccattttt atcaactagt tcacagtatc
    18961 ttttggcaat atctattgca agttcatttt caccgcattc ttctaaagcc tcaacaaata
    19021 atagcgtagt aggagcccaa ataggacctc tccaataagc atcatcttga tattcatcac
    19081 tatctatagc ttccgttgcg attccccatt tagttaaata tctttcacta ataattaaat
    19141 ctatcatttt gtctctagta tatttaggaa gtctatttcc tagcactaaa gaaacaagag
    19201 ggagtaaagc attattagga agtattttac cagttaaagt ctcacgagct acaggtaaat
    19261 catcaactat aaaataactt aagaataatt ttgtaagctc atctgcttta ttagcccaat
    19321 actctttttc actatattta tttagttttt cagctacttt tgctaacatg tccatagcat
    19381 ttattataaa tgctgttaag tcaggcgtat caacaaccat gactccttta aatattgtac
    19441 tattatcttg accagaatca tttccatgat gatattcaaa aataccatcc atgtttgaat
    19501 ctttaaactt tatataaaac aatacttgct tttctatgta attataaatt tcctctaatg
    19561 tttctttagt aaagttaatt tttttcatca ttttattaaa gaataatccc tgaataggtg
    19621 gttttgtaaa attccatcta atagtagaat cacttaaaga ccctggaatt tgaccatatt
    19681 catcttggta gtcgaaaagt actgccattt ggtcaaaagc taatttatca tctacaccag
    19741 ctaaagccaa tgcgttaaaa cagtgatccc aactccatgc ccctaagaat gttgagtttg
    19801 aagcaagtat agtatatctc tttaggtatc cttgaggttt aacgaaagaa gaccaattta
    19861 tataactagc tcttaaaagt gaatttttat acttttcatc tatcttaggt tgagctagat
    19921 aatattcatt aaataatttt tttgtgttat ttaatgaagt ttcaaaatta tattccttat
    19981 aatctggaac cttcatatga gtgggaatat cctctataat acatgtaaat tcattatcag
    20041 tattattgtt aacatctatt attgaagtgt tgttagcttt attggtagaa gcacagtcat
    20101 ctataaatat attttgtgat aaattaacct taccatcaac agagaatact aaaaacttag
    20161 ttaaattttt gtagctattt atcacacaat attctgaatc ttttttcccc ataagatagt
    20221 tatattcaaa attgtataca ggaagtgtat ctaatttaag ccccacattt tttcccttac
    20281 cgtagaataa gattttagaa tctccatcaa aacatataga tatgctacca ttgttgaatt
    20341 taattaatat actgaaataa tcagcctcaa attcataatc tacaggtatg ttatttacag
    20401 taggtataat ccttagtaca tccatatttt gttttgattt tccccttagt gattttaatg
    20461 taaatccaag ttcatctgaa taagaaatag acatataaga cccaaagtaa ctaaatggaa
    20521 tttcttttat attcatttgt agtatattcc tttcataatt tattttatat agaatctaac
    20581 ttgtttatac aagtaagtat atatttaact taccaaataa taaaataatt atcaagagtc
    20641 aatttatcaa aaaaataaat aggaaactgt tttcaaaaaa ctattgacta aattaatgca
    20701 aaatattaca ataaagttgt tcaaacaagt acttgtctaa acaacttgtt ggtaatgatg
    20761 aaaaaaagaa aggataaatg aatatgggga agaataagat attaaattca aaagaaagca
    20821 tttctcaaat tattgagtat gttgggggaa aagaaaatat acaaagtgca acacactgtg
    20881 taacaagatt acgtttaata ttaaaagata ctaacaaggc aaacattaat aaactagaag
    20941 gcattgatat agtaaaagga tgtttcaatg caggtggaca gtttcaaata gtaataggtg
    21001 tcggagtaga taaagtatat aaagatttaa tagaacaaac aggagcagaa gaagtatcta
    21061 aagaagaaat taaacaacaa gtcaatgaaa agtcaaataa attacaaaag tttataagag
    21121 ttcttgctga tatattcatt cctatattac cagcaatagt tgcatcaggg ttacttatgg
    21181 gtgttaataa catgatagct aatccaggaa ttttttatga gcagtctgtc atagaggtaa
    21241 atactggtat agctggaatt tcaggtatta taaatttaat agcaaacaca tcatttacat
    21301 ttttaccagc attaataggt tggtcagctg ccaagaagtt tggaggaaat cctttgttag
    21361 gtgtagtttt aggtcttgta ttagttcatc cagctttgat gtctgcatat gattttgcgg
    21421 caaatccatc cgcagcacca acatgggata tatttggtat gaaggttgct caagttggat
    21481 atcaaggtca agtaatacca gttttaatag ctacatacat tttaactaaa cttgaattat
    21541 tcttaaaaaa gactgtccca gatgcagttc aattagtatt agtttcacca atagctttat
    21601 taattacagg catgttaact tttgtagtta taggtcctat aactatgaca ggtgcaaata
    21661 tgataactaa tggagtagtg catttatttg aagtagcacc agtttttgca ggagcattat
    21721 attcatttat ttcaccacca ttagtaataa caggaatgca tcatttattc ttaggagtaa
    21781 acttacaaat ggcagggact ttaggatatg taacattgtg gccaatatca gagactgtta
    21841 ctttagctca aggagctgct acattagcaa tgtttttcat acttaaaaat aataaaaact
    21901 taagaggtgt atctattaca tctactatat cagcatggtt aggaataaca gaaccagcta
    21961 tatacggtat aaacttaaga tttagatatc catttatagc cgttatggta gcaagtgcaa
    22021 taggaggagc atttatatca tatcacaatg ttaaagctac agctgttgga gttggaggag
    22081 tattttcatt cctttcagta atgcctcaat attggggagt atattttaca ggaatggcaa
    22141 taacatttgt aataacaatt ggattaacat acttatttta taaaagtggt aaatttaaaa
    22201 caaatgacaa catataaata taattccctt gattttatat cagggggtat taatatgtat
    22261 tggatagata ggagacaata tgttagattt taaaaagagt gtagtgtatc aaatatattt
    22321 aaagtcgttt aatgatacaa caaataatgg tttaggagat ttaaatggaa taacagaaaa
    22381 attagattac ttagaatttt taggtgttga ttatgtgtgg attaccccat tttttgtatc
    22441 accacaaaat gataatggat atgatgtagc tgattattat aatatagacc ctatatatgg
    22501 aactatggat gatttggaaa atttaataaa agaagctaaa aaaagaaata tgggtatcat
    22561 gcttgatatc gtatttaatc atacgtctac agagcatgag tggttcaaaa aagcacttaa
    22621 aggagataag tattataaag atttttatat atttaaaaat cctaaagaag gtaagagtgc
    22681 cccaacaaat tgggaatcaa aatttggagg caatgcttgg gagtttgtag aaaaatttaa
    22741 tcaatattat ttacatttat ttgataaaac tcaagcagac ttaaactggg aaaatcctaa
    22801 tgtaagaaaa gaattaaaaa atatactaaa gttttggata gataaaggta taaaaggatt
    22861 tagatttgat gtagtaaatt taatatctaa accagagaaa tatgaagatg atttacaagg
    22921 tgatggtaga catttttata cagatggtag aaaagtacat caatacttaa aagaaatgtg
    22981 tgaagaaagt ggagtattgg aaaatgatat gttaaccgta ggagaaatgt cttctacgac
    23041 tatagaaaac tgtataaaat attcaaatcc aaatgagaaa gaattgtcta tgtgttttaa
    23101 ttttcatcat ttaaaagttg attataaaaa ccaaaataaa tggatgttgc aagattgtga
    23161 ttttattaag ttaaaaaaac tacttaatga gtggcaaata ggaatggaaa aaggtaatgg
    23221 atggaacgct ttattttggt gtaaccatga ccaaccacgt ataatatcta ggtttggaga
    23281 tgataaaaag tattataaag aatcggctaa aatgttagct actatgattt atttattaag
    23341 aggaacacca tatgtatatc aaggtgaaga aataggcatg actaatgcat actttactaa
    23401 tataaaacaa tatgtagatg ttgagagttt aaattatttt gatatattaa aatctcaagg
    23461 aatgaaagat ggcgagatat acaatatatt acaggaaaga tctcgtgata actcaagaac
    23521 acctatgcaa tggaataata gtaagaatgc aggttttagc gaatcaaaac catggataga
    23581 agttataaat aattatagaa atataaatgt agaaaatagt ttaaaagata aggattctat
    23641 tttttattat tataagaagt taatagaatt aagaaaaaag tatgatgtag tagcatatgg
    23701 agattttaag ccattattag aagagaatga ttctatattt gcatataaga gatgttacga
    23761 aaaagaatct ttagtagtgt taaataactt ttatgataaa gaaattgatg ttattttaga
    23821 taatgaagag cttgaaaaat acaagtgtat aatatctaat tatggagacc gaaaagttac
    23881 ttcaacaatt accttaagac cttatgagtg catggcttta tttaaaaatg aaaaatagtt
    23941 taaaattaaa attaagaaaa atttatgtgt actctttaaa taaattttat ttttgaagtt
    24001 ataatttaat attta
//
LOCUS       DAFKMX010000043        23733 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFKMX010000043 DAFKMX010000000
VERSION     DAFKMX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.043"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            2..1042
                     /gene="mltG"
                     /locus_tag="LZB08_001377"
     CDS             2..1042
                     /gene="mltG"
                     /locus_tag="LZB08_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBM7314887.1"
                     /translation="MIVILIILTGIFVFIQIGPYDKNNKKDVIIDVPSGASVGKISDI
                     LYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISLLVSGKIYQDGIKV
                     TIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKEDGITSLEGFLYPE
                     TYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVMEMASIIEKEAVLD
                     KDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKIDSPYNSYKNKGLPP
                     TPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKYVKEYKEARDKQSK
                     DTKATNKENTKK"
     gene            1214..1888
                     /locus_tag="LZB08_001378"
     CDS             1214..1888
                     /locus_tag="LZB08_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBM7314888.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            1875..3122
                     /locus_tag="LZB08_001379"
     CDS             1875..3122
                     /locus_tag="LZB08_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBM7314889.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            3194..4864
                     /locus_tag="LZB08_001380"
     CDS             3194..4864
                     /locus_tag="LZB08_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBM7314890.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(4961..5602)
                     /gene="sigK"
                     /locus_tag="LZB08_001381"
     CDS             complement(4961..5602)
                     /gene="sigK"
                     /locus_tag="LZB08_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBM7314891.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            6169..6531
                     /locus_tag="LZB08_001382"
     CDS             6169..6531
                     /locus_tag="LZB08_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314892.1"
                     /translation="MKIRKRGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            6515..7231
                     /locus_tag="LZB08_001383"
     CDS             6515..7231
                     /locus_tag="LZB08_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314893.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWTGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEAVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            7251..7670
                     /locus_tag="LZB08_001384"
     CDS             7251..7670
                     /locus_tag="LZB08_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314894.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            7660..8184
                     /locus_tag="LZB08_001385"
     CDS             7660..8184
                     /locus_tag="LZB08_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314895.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIIGNSMGIMNISNYEETFKKTTSIKCRYVDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKMKVWNFSESI"
     gene            8496..9053
                     /gene="efp"
                     /locus_tag="LZB08_001386"
     CDS             8496..9053
                     /gene="efp"
                     /locus_tag="LZB08_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBM7314896.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            9145..9600
                     /locus_tag="LZB08_001387"
     CDS             9145..9600
                     /locus_tag="LZB08_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBM7314897.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            9763..10473
                     /locus_tag="LZB08_001388"
     CDS             9763..10473
                     /locus_tag="LZB08_001388"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBM7314898.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            10527..11597
                     /locus_tag="LZB08_001389"
     CDS             10527..11597
                     /locus_tag="LZB08_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7314899.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNINQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            11625..15179
                     /gene="smc"
                     /locus_tag="LZB08_001390"
     CDS             11625..15179
                     /gene="smc"
                     /locus_tag="LZB08_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBM7314900.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLESSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     NISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            15206..16486
                     /gene="ftsY"
                     /locus_tag="LZB08_001391"
     CDS             15206..16486
                     /gene="ftsY"
                     /locus_tag="LZB08_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBM7314901.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            16589..16957
                     /locus_tag="LZB08_001392"
     CDS             16589..16957
                     /locus_tag="LZB08_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBM7314902.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            16954..18306
                     /gene="ffh"
                     /locus_tag="LZB08_001393"
     CDS             16954..18306
                     /gene="ffh"
                     /locus_tag="LZB08_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBM7314903.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            18344..18616
                     /gene="rpsP"
                     /locus_tag="LZB08_001394"
     CDS             18344..18616
                     /gene="rpsP"
                     /locus_tag="LZB08_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBM7314904.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            18681..18908
                     /locus_tag="LZB08_001395"
     CDS             18681..18908
                     /locus_tag="LZB08_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBM7314905.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIILKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            19016..19531
                     /gene="rimM"
                     /locus_tag="LZB08_001396"
     CDS             19016..19531
                     /gene="rimM"
                     /locus_tag="LZB08_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBM7314906.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            19540..20235
                     /gene="trmD"
                     /locus_tag="LZB08_001397"
     CDS             19540..20235
                     /gene="trmD"
                     /locus_tag="LZB08_001397"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBM7314907.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            20420..20770
                     /gene="rplS"
                     /locus_tag="LZB08_001398"
     CDS             20420..20770
                     /gene="rplS"
                     /locus_tag="LZB08_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBM7314908.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            21091..21987
                     /gene="ylqF"
                     /locus_tag="LZB08_001399"
     CDS             21091..21987
                     /gene="ylqF"
                     /locus_tag="LZB08_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBM7314909.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            22376..23668
                     /gene="brnQ"
                     /locus_tag="LZB08_001400"
     CDS             22376..23668
                     /gene="brnQ"
                     /locus_tag="LZB08_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBM7314910.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         9997 a         2307 c         4353 g         7076 t
ORIGIN      
        1 aataattgtt attttgatta tcttaacagg tatatttgtg tttatacaaa taggtcctta
       61 tgataaaaat aacaaaaaag atgtaattat cgacgttcca agtggagcat ctgttggaaa
      121 aatatctgac atattatatg aaaataaatt aataaagaat gaactattat ttaaactttt
      181 ggtaaaggtt agcaataaag ctccgtctat aaaatcagga acatacttac ttaatcaatc
      241 gtattcaaac aatgatataa taagtctttt agtttcaggt aaaatatatc aggatggtat
      301 aaaagtgact attccagagg gagctacatc taaagaaatt atagcaatgt tagtaagtaa
      361 aaatttagga gataaagcta cttttgaaaa tttaataaaa aaacctcaag aattttatga
      421 taaatttcca tatttgaagg aagatggaat aacctcttta gaagggttct tgtatccaga
      481 aacttattat tttaattcaa agaaacaatc agaagaagat attttaagcg aaatgttaaa
      541 agtatttgac agtaaataca cggacaagtt taaaaagaaa caaaaagaat taaatatgac
      601 tcttcaagaa gtaatggaaa tggcatcaat aattgagaaa gaagctgttc ttgacaaaga
      661 cagacctata atagcaagtg tgttttataa tagacttaag gtaggtatgc ctcttcaatc
      721 agatgctaca atacaatata tatttgaaga gagaaagaaa attgtaacat atgatgattt
      781 aaaaattgat tcaccatata atagttataa aaataaggga ttacctccta caccaatatc
      841 aaatcctggt ataaaatcta ttgaagcagc attatatcca gagaaaacag actatttata
      901 ttttgtagct aagattgacg gtggaaataa ttatagtaca aactatcaag accatttaaa
      961 atacgttaaa gaatataaag aagcaaggga taaacaatca aaagatacaa aagcaacaaa
     1021 taaagaaaat acaaaaaaat aaacaatgat tataaaaagt ctaacagggt aagaataatg
     1081 ttgggctttt ttattgaaaa ttgttattct tagatttata ataaagaaag tgtaaatata
     1141 tataacaata tgaatttaaa aaataataaa ttaatataaa acttgaatat ttattatttt
     1201 agggagtgtt tttatgagta atatagttaa tgatttggtt gaagattata taagagtgac
     1261 attaaaagaa aaagaaggat ttttaaaaga tttagaaatt tatgcagagg aaaatagtgt
     1321 tcctataata cataaagaag tttcagattt attaaaagtc ttgttaaagg ttcaaaaacc
     1381 aaagagaatt ttagaagtag gatgtgcaat tggttattct tctatttttt ttgcaactat
     1441 tataggaaaa gatgcagata taataactgt tgagagaaat gaaaaaatga tagaaaaagc
     1501 aaaagaaaac ataaaattag ctggatttga caataacata actatactag aaggagatgc
     1561 agaagaaaaa cttagtcaag tccaaggtga atttgacctt atatttatag atgcagccaa
     1621 aggtcaatat aagctatttt ttgatttagt tatagataaa ttaaaagatg ggggactttt
     1681 agtatctgat aatattttgt ataaaggtat ggttgctcat gatgattttg ttgtaagaag
     1741 aaaaaagact atagttaaaa gaatgagaaa ttatttagat tatatatgta attgtgacta
     1801 tttaagcaca tcattggtac caataggtga tggtgtagca ctaagttata aagaaagtaa
     1861 aagaggtgat gtagatggaa ttaaataaag tagaactttt agctccagca ggtgatttgg
     1921 agagattgaa aatagctata acatatggag cagatgccgt ttatataggt ggagaaattt
     1981 ttggaatgag gtctgcagcg aagaatttta gtaaggaaga tatggcagag ggagtagctt
     2041 ttgcacatga aagaggcaag aaagtattcg taacagtaaa tataatacct cataatgaag
     2101 attttctaca attagaagac tatttgttgg aactagaaga gataggaata gatgcagtta
     2161 taatagctga cccaggagtg cttagtgtca ttaaaaaagt aattccgaat atggaaatac
     2221 atttaagtac acaagcaaat acaaccaatt atctaagtgc aaacttctgg tacgagcatg
     2281 gaataaaaag agttgtagtg gcaagagaat tatcttttga tgaaatatct gagataagag
     2341 caaaaacacc tttagatatg gatatagaag cgtttatgca tggtgctatg tgtatttctt
     2401 attctggcag atgtttaata agcaactata tgactggaag agatgcaaac aaaggttctt
     2461 gtgctcaatc ttgtagatgg caatatcatt tggtggaaga aaaaagacct ggtgagtatt
     2521 ttccaatata cgaagatgaa aggggtacat tcttctttaa ctcaaaagat ttatgtatga
     2581 tagagtatat accagagtta atcaaatctg gtataacaag tttaaaaata gaaggtagaa
     2641 tgaagactgc atactatgta gctactgttg taagagcata cagaatggca atagatgaat
     2701 tttacagaga cccagaaaac tggaagttca atccaatgtg gatggaagaa ctaaaaaaag
     2761 gtagtcatag acattttaca tcaggttttt acttgaataa accaacaaca gaagaccaaa
     2821 attatcaatc agcatcatat gttagaaact atgattttat aggtatagtt agagaaacag
     2881 aagatgaaga tggtcttatt gttgttgagc aaagaaataa aatgtgtgtt ggtgatgaaa
     2941 tagaagttat gggaccatat aaagaaacta tgtttactaa aattgaagaa atgtacaatg
     3001 aagaaggaga agctatagaa tcagctcctc atcctagaca aatagtgaag ttaaagctat
     3061 cagtaaaagt tggtaaagat tatatgctta gaaaagttat agaagaaaag gttgaagaat
     3121 aataaaaaat tgataaaaat agtattagcc ccttagaaat tgtcaataat gaaattatct
     3181 aaggggtgat ttcatgtcaa aaaagaagac tccatttttg aaaaaagtgg gaaagagaag
     3241 ctggtgtata tttactataa ttttaataat ttatagtgtt ttaatttata ggctagttga
     3301 tatacaagta ttaaaaggtg ataaatacaa acaaagtgtt gaatcacaaa gcgttgagaa
     3361 agtcgagtta aatagtggaa gaggaataat ctatgataga aacaacaaga agcttacaga
     3421 tacaagtaaa tctcaggtat taattgttga aaaagaaaaa ttaaataata actataaaat
     3481 tttggagctt attaagaaag ctactaagat gaatgattta gatatatata aagctgtaca
     3541 agagcaattg acacgcccta taatacagat tcagactaaa aatatcgata tcgataagag
     3601 tatgaaaaaa gaacttgaga aaaatggaat aatggtagaa gaaaaaacta tgaggtacgc
     3661 aaaagatggt ttattatcac atacaatagg atatataaaa gaagatgata aatcaggtca
     3721 atcaggaatt gaaaagagta tggatagtgt acttagaaat tcaaatgaaa agtatataag
     3781 tgcttttaaa gctggagatg caggaaatga aaaatctcta aacatactaa agggaagtgt
     3841 aaaaactgtt gataataagg ataaagatag acatctaaaa actactattg attataacat
     3901 tcaaaaaaaa ttagaacaaa tactaaataa agaagaaaat cctacagcag caataatctc
     3961 ggaagcatct acaggtgaaa ttcttgcaat gtgctctaga cctaattttg accaaaatga
     4021 catatcaaaa agtttaaaag gaaaaaatgg agaatttgaa aatagagtta taaaagcgac
     4081 atatccacca gggtctgtat ttaagatggt agttttattt tctgcacttg aaaatggtgt
     4141 tatagatgag aactatactt acaactgtac aggtaagact aaagttggga atactaatga
     4201 aatactgcga tgtaataaaa gagatggtca tggtttccaa aacttaagac aagctttttc
     4261 taattcatgc aatcctgcat ttttagatat agctatgaaa cttggaaaag aaaagatact
     4321 caaatctgct gaaaaattac atttatttga aaaggttgat attggtcttg atgaagaaaa
     4381 aattagagaa gctccaaaaa acatatcaat aagaaattta gctataggtc aagaaaatat
     4441 agaatttaca ccacttcaaa taaatcaaat gactcaaata atagctaata atggaacatt
     4501 caaaccacta tacttatata aaagtcttgt ggacaataat atgaatacaa taaaaactta
     4561 taagtcttca aaaaaagaag aacttatttc tccttatgtg tgtacacaag ttaaagaata
     4621 tatgaagtct gtatctagga ctggtacagc taaagaccta aaagatattg aaggtggttg
     4681 tggagttaaa actggtacag ctcaaagtag tttaaataaa aaagctatag accacggatg
     4741 gataacagga ttttatcctg aagaaagacc taagtatgta ataactgtgt tagtagaagg
     4801 aactcaaaaa ggaaataaat ctgcaacacc tatatttaag gaaatatgtg aaagtataaa
     4861 ataaattatg tactataaaa tttaaggata tatcgtaact ttgattttag atagaaaatt
     4921 ttgtctaatg atgtacgata tatcctttta aaatatagta ttaactttct tgaacaagct
     4981 ctttttctag ttttttaaga gctttttttt cgattctaga tacatatgac cttgatatat
     5041 cgagtttttg tgcaatttct ctttgtgtct tatagccata aggagttaac ccatatctta
     5101 actggacaat ttctctttct ctaggagtaa gaattttgtt tagttgttcg taaagttttc
     5161 caacttgaac ttttagttca acttcatcaa gaacatagtt agcctctgta ccaagtatat
     5221 ctaaaagact tatttcatta ccttctttat ctgttccgat agggtcttgt agagatactt
     5281 gagttttatt ttttttgtta gatctaatat tcattaaaat ctcattttca atacatctag
     5341 aagcatatgt tgctaatcga gttcctttat ctatgttata agtttctata gctttgatta
     5401 atcctattgt acctatggat attaaatcat cctgatcttc cgttgaatta ttatatttct
     5461 ttgctatata agctactaat ctcatgtttc tttcaattaa tgtatctttt gcacttttat
     5521 cattttctat tttaaattta gttaaatact caatctcttc ttcaggggtt aagggttttt
     5581 caaaagattt aagagctgcc aataggtcac ccccttttca acggtcactt taaattatat
     5641 gaaggagact tatccaagtt gactgttatc taataaaata atatgcatat ttttagaatt
     5701 taaaacatat atttgcaaac ttttgaatgg aatatggcat aaagagtggg aatttaaatt
     5761 taagtttttg ttgtatatat atgaaacaag gcgaagaaat tattgattta taaagtataa
     5821 attggaattg aggtgtcaat aaatataaaa ggaaatagta tttatatatg ttttggtgtt
     5881 gaagtaaaag attaatgcaa agcattagag tctataaaaa tatgaatata ggaggtgtaa
     5941 ttttacaata cttaaaaaag taaatgtaat taattatttg tataaagttg attacaagca
     6001 aaagcatcag tcatagttaa tactgttaag gatgaaggag gtgtgttaat ttatatatgt
     6061 atgggaagaa attttgatta caataaaaaa acttacaaaa gatatttatg aaaaaactta
     6121 tagttaaatg tttatatata aaatattatt taggggttgt ttttattatt gaaaataaga
     6181 aagagaggtt ttatatcaat cgaatgtata ataagtattg ctatattata tgtggctgtt
     6241 tatttagttt ctacatcatt gtataattgt tatagtttta tcagtagaaa tatatctgac
     6301 agagaaatgt taagtacagc aaaaaaatat atagaagatg agaagtatag aatacaaaat
     6361 agtaagtatg agttaattga agataagata gaaaaaaatt acataaatgg atatgaaatt
     6421 aacagtagaa tagagcaaat tttagattat tatcaatgct atgaaataaa tatagagata
     6481 aaaaatgaat ttaaaaaact gaggtttaat agctatgtta ctagaaaata aaggctctat
     6541 attaataata acattgataa tattttctat tataagtact gtgtgcatta tgtgtatagg
     6601 tttaatttat acacataata atatgtttga gttaggatac aaagacattc aactaaaaga
     6661 aatttcttta agtggaatag aaatatcagt aagtaatgta ctttcttgtg ttgaggaagc
     6721 tattgatgta tctaataatg aagacgaatt tagagaatat ttcttaggaa ataatatgat
     6781 tgaatgtata aaaaaaataa aagatacatc ttatagtagt ttagaagaac tttctttaaa
     6841 agtagaaaat aatactattt atgacagagg agattttttt gaatttgata tagaaagtaa
     6901 tgttagagaa aaagaatttt taaaagcaat aaaagtaaga gtgaaaatca aaaatccatg
     6961 gacaggaatt gatattgaag attatatgga taaatctaat aacaataaaa ttgatgacaa
     7021 aacattcaat aattataaag catctgaaaa taatgaaatt ctaaatcaat ttaaaaatga
     7081 aagtgaaaat ataaggaatc aagaggctgt tgaatttaca gaagaagatg aagaatatga
     7141 agaagaactt aaacaaaaca taattgaaaa tataaagaag aattttgatg aaaaaaacct
     7201 agtacaggta tataagtata ggggtgtata actttgaaaa aagaaggaat gtgactgtaa
     7261 tagaattaat agtatctatc agtataatac tcttgatagt tacattatct tttccaaaag
     7321 ataatcttga aaatcataaa ataaatttat ttgcacgtca actttgttca gatataaggt
     7381 atgttaggcg aattaatatg tttagcaact acaatacata tatatattat ataaatcaag
     7441 gtaaacacga aggttatgta ttgagaaaag atggtaaaaa cattaaatca ataatgctac
     7501 ctaaaaatgc aaaaatagct aggagtattg aaataataaa gtttaaatct gatgggtcgc
     7561 ctcaaaaggc gtctacaata gagatatata ataatagatt aagaaaaact ataacaataa
     7621 cacctgtaag tggaagagta cttctgaaag agggaaaata tgaaacataa atatggttac
     7681 ttgcttttgg aaagtgtagt atcattgtct tcaatggtga taattatact agttttatat
     7741 tctatatttt tgtctacaat aaacttaaag ttaaaagtag aagataagat agaactacaa
     7801 caacaaagtc ttgaaatcat aaaatctatg gaaggaatta ttggcaattc tatgggaata
     7861 atgaatatat ctaattatga agaaacattt aaaaagacaa cgtctataaa atgtagatat
     7921 gtagatgaaa ataataatga agaaggcata agtaataaag aaattatatt gaatgaaaga
     7981 cgaaataagt tatttgtaaa ttctttaaat ggcgaaagta gtcaagcagg tggttatgaa
     8041 attggagatt atgtagatga aatgtatgta tcaattacta ataatggtca atacgttaat
     8101 attaaattga aattatcaaa acgtagtcaa aaatatgaga ctgatttcaa aatgaaagta
     8161 tggaatttta gtgaaagtat ataaaaatac ataaatttaa gagaaagaaa tgacatcaat
     8221 aagtcatata aaatataatt attgagaact atataactaa ataatatcaa tatgtaaagt
     8281 gtatagatta agaatataga ggataataat aaaaatatag aataagtaat ttgccaaata
     8341 cttgtttata ttgtataact tatgatatta tgatattagt aaaaatttgt aatcctgact
     8401 tgacagaatt caagtaagag atataatttt aaaggcatag taatgtaaaa tttactaaaa
     8461 aataagaaat ttattttaat atggaggaac aaaaaatggt atcagcaggt gattttagaa
     8521 aaggtgttac atttgaaaaa gatggacaac catgtttagt agttgatttt caacacgtta
     8581 agccaggtaa aggagctgct ttcgttagaa ctaaatacaa aaacttaaaa acaggagcta
     8641 taagagaaga aagtttcaat ccaagtgaaa aattccctaa agctgttata gatacaagac
     8701 aaatgcaata tctatacaat gatggtgagt tatattattt tatggatcaa gaaaattttg
     8761 aacaaatacc attaaactat gagcaagttg aagatgcaat taagttctta aaagaaaatg
     8821 aagttgctac aataagattt taccaaggac aaccattcca agtggaagcg ccaaactttg
     8881 cagagctaga agttacagat acagagccag gtataaaagg tgatacagca agtaatgtaa
     8941 ctaaagcagc tacagttgaa actggagcag ttgttcaagt tccattattt ataaatactg
     9001 gagataaagt aaagatagat actagaacag gtgaatattt atcaagagta taaaaattta
     9061 tgtatataaa agcttatatg tcttaataat ataagatata tgagctttta gtttatatat
     9121 aaaaaagaaa gcgaggggtg ttacatgaaa catttatatg aagaagttgc atacttaaaa
     9181 ggcctagcag aaggactaga aataagtgct gaaagtaaag aagggaaaat gatacataaa
     9241 atagttgatg cattagaagt atttgcagat gctattgtaa cattggatga agaacaagaa
     9301 gaacttcaag attttgttga atctatagat gaagatttag cagatttaga agaagatatt
     9361 gctgacatgg aagaagatct ttatgaagaa gatgacgatg aggatgatga ggacttcagc
     9421 tatatagaaa tggaatgccc aaattgtgga gagttagtag aaatagacga agatttgtta
     9481 tatgatgatg aagtagatgt tgtttgtcct gattgtaagg ctgtgatatt atcatcagaa
     9541 gatgattgtg atgatgattg tacatgtggt ggttgttcaa gttgtgatga tagagagtaa
     9601 aaattcataa gaattaaaac tgcctatgat gcctatgaga tattttgctt cataggtagt
     9661 tttattttta tgaggaaggg tattatgtat tttggttttg caatacttgg acaaaaatag
     9721 tacactagaa tttatagaat aggattttgg agggatgtaa atatgaaaat aagtaaaaaa
     9781 ttactagata atatacaaag atttgaaaat gttataaatt ataaatttaa aaataaagaa
     9841 tatatactag aagctcttac tcatagttca tattctaatg aaaataaaaa atataatttt
     9901 aacgaaagat tggaattttt aggggattcg gttttaggca tagtgataag tgattattta
     9961 tttaatgaag aagctaatct gcccgaaggg gaactaacta aacttagagc aaatatagtt
    10021 tgtgaagatt cattaagtga agtagctaat gatataaatt taggtattca catgttatta
    10081 ggtagaggag aagaagcaac aggaggaaga cataggactt caatattagc agacgcattt
    10141 gaagctgtaa tagctgctat atatcttgat ggaggttttg aaagtgcaag acagtttata
    10201 cttcatcata tggaaaatat aatttatgat tcaagaaaag gaaatatatt tagagactac
    10261 aaaacgcatc ttcaagaggt gttacaaggg aatggagaaa ataatatctg gtacaggctt
    10321 atagaagaaa aaggaccaga ccataataaa agatttgtaa tggaagttgg aattaatgac
    10381 gatgtacttg gtataggaga aggcaagagt aaaaaagaag ctgaacagtt agctgctaaa
    10441 atagctttaa agaaaaaatt atgggaaaaa taaatataat atgtaaaaat ataaaaagaa
    10501 atatttatat tagaaggatg ttgtagatga aaaagagaat tatacctata tttgttcctc
    10561 acaaaggatg tcctcatgat tgcatatttt gtaatcaaaa aaaaataaca ggtgtgagta
    10621 ctgatgttac tagtgaagat gcaagaaata taattgagga atgtttagag actatagata
    10681 aagatgcaga tgtagaaata gcattttttg gtggtagctt tactgctata gatattgata
    10741 tacaaaaaag tttattatca gttgctaaag aatatgtaga aaaaggtctt gtaaaagata
    10801 ttagaatgtc tacaagacca gattgtattg atgaagatat attaagtatg ttaaaagaat
    10861 ataagacaag tataattgaa ctaggagtac aatccttaga tgaaaaagta ttattagata
    10921 gtataagagg tcatcaatct gaaattgtat acaagagttc aaagatgata aaaaatagtg
    10981 gaatcaaact tggattacag atgatgattg gtcttccagc tgacacagaa gaaaagtgta
    11041 tatttacagc aaaaaaattt gtagagctaa aaccagattg tgttagagtc tatccaactt
    11101 tggtagtaaa agatacaggt cttgaaaagt taatggaaca aaataaatat aatccattta
    11161 ctcttgaaga gagtgtaaat atagtaaaaa aagttcttgt actgttttat gttaataata
    11221 tcaatgtaat aagagttggg cttcaagcta ctgatgatat acaaattgga aaagctgttt
    11281 tagcaggacc atatcatcct gcatttagag agttggtaga agcagatatg ataaaagatt
    11341 atttagaatt tgttattttg caaaataaaa atataaatca aatgttggtc aaatctaata
    11401 aaaagaatat ttctaaaatc ataggaaata aaaaaacaaa tgttaaatat atggaagaaa
    11461 aatttggtgt gcttttaaaa acacaagaat cagatttaga tataaatcaa ttagaaattg
    11521 tgttagatgg gaaaagttta attagtgcta atatgagaga cattcataga aaattgtatg
    11581 acatttatga cctttagttt taataatgaa gggagagaaa agatttgtat ttaaaaagac
    11641 tagagttaaa gggatttaaa tcctttccag taaaaacaga tattattttt aaagaaggaa
    11701 taacagccat agtaggacca aatgggagtg gaaaaagtaa tatatctgat gctgtaagat
    11761 gggtattagg agaacagagt attaagagtc ttagagggga taaactcgaa gatgtaatat
    11821 ttgcaggtac agatacaaaa aaacctatga attattgtga agtggctctt actattgata
    11881 attctgaaaa tcaattggaa ttagatttta cagaagtaac tataagaaga agggcttata
    11941 gaaatggtga aagtgaattt tttctaaata ataaaagttg tagattaaaa gatataaaag
    12001 aagtcttttt agatactggt attggaaaag atggttattc aatcatagaa caaggaaaag
    12061 tagatgaaat attaagtaat aatcctttaa gcagaagaaa agtttttgat gaagcttgtg
    12121 gcatatcaaa atatagatat aaaaaacagg aagcagaaag gaatttaagt aatacaaagg
    12181 aaaatttaga aagaatagat gatgtatata tagaaattga aaatcaatta aagcctcttt
    12241 ttaatcaaca gacaaaggca aaaaagtact tggaaataag tgaaaaatta aagacacttg
    12301 aagtaaatag ctttataaga gaaattgaag gaatagaaaa agaattaagt gaggttaatg
    12361 aacatcgtaa ggttattgaa aaggagctca atgaaaaaga agaacaaaaa aatgttgtag
    12421 agaaaaagca agaagatatt aataaagagg tagaggtatt acaagatgta attgaaaaat
    12481 ctgtagatta tataaattca attaaaggag ttatatcaaa aaaagaatcc caaataaatt
    12541 taattaagga aagaataaga aattttacga atgaaattag tagaaaaaat ttagaaataa
    12601 aggatataaa agagaagcta aatgaaaata agcaatacat aaaagaatta gaaagcaata
    12661 aattatcagg tagtgaagaa ctatctacct tgcaggaaaa tataaaagta ttggaatcaa
    12721 gtaaagataa acaaaaaatt aagttagaat ccctaaataa tgagatagaa ttattgaaag
    12781 aaagtataat tgatatatta aataagaaac aagagtttag taataagctg tccactttaa
    12841 acgcaaataa agaaaatatg aatatcagag atgaaaatat aaattcagag ataacagagt
    12901 taaacaaaaa tattgaaata aaatcatctg aattagatac tataaataaa gaatttaata
    12961 tgcaaaatga aaatttaaag aatgtaaata atagacataa agaattatca attaatttgc
    13021 aggattcaat tagtgaacac aataaactag aagatgaaat acagaaaagt aaatataatt
    13081 taaatggata taattcaaag ttaaatgtct atatagatat ggaaaatcat tatgaaggat
    13141 ttaatagagg agtaaaagaa gtactaaaaa ataaaaattt aaaaggagtt catggagcat
    13201 taggacagat tataaatgta cctgaaaagt atgagaaatc tatagaagca gctcttggtg
    13261 cttatatgca aaatataata actgataatg agtttagtgc taagtctgca ataaactatc
    13321 taaaacagaa taatttaggt agagtaacat ttcttccatt aaatataatt aaatcaaata
    13381 aaataagtct aggaaattta aaagcaaata ctaagttcat aggaatagca agtgatttaa
    13441 ttacatttga tgaaaagtat agaaatataa ttgaaaatat acttggaaga acaatactta
    13501 taaataatat agatgagggc ataaaatttg caaaagaaac tggtcatagg tttaaaatag
    13561 taactttaga tggtgaaatc ttaaatccag gaggttcttt gactggggga agtctaaaaa
    13621 ctaatgggaa tatactatcg agaaaaagat acattaatga atatacagaa aacataagta
    13681 atatcaagaa tgaaatttca catctagaat taaaaagaga atctttagac aaagatgtga
    13741 aaaatatcaa aaatgaaatt gattcacatg aaagtaaaat aaaagattta gaaaaaagta
    13801 taataataaa aagtacaagt ataaagaatg ttgagtctga aattgaatcg ttaaaaggta
    13861 gtattactaa attagaaaat gagaagaatg atttaaattc taacctaaat tatacacttg
    13921 aaaaaagtga tgatgttaga aaagatatgg aagagttaga tgatttatac aataaaaata
    13981 aagaaaaaat tgatgcattg aatgaggaaa taaaaagata caatgattta tatgataaag
    14041 aaaaatctga atttgatgaa ttgaacttaa gtttagtgaa gaaaacagaa gtttacaata
    14101 gtatagttag agatataaaa agaatatctg gtgaaaattg tgagctagaa gaaaaaaata
    14161 aacaattaga agagtcatta aattatgaag aacatgaaat aattaaatta caagattcta
    14221 tactcactga agaaaaagaa aaagaaaatt taacaaaaca actaggggat agtaatagaa
    14281 atcttgaaac tagaaaaata gcaaaagatg atttaaaaaa tagttttgat gaaattaata
    14341 aagaattaaa aactatagat agacagcata tagaacttaa agaaagttta tttaaagttg
    14401 gtggaagatt agaaagatta aaaactagtc aagacacata tataaataag ttgtttgagc
    14461 aatatgatat gaccttagta caagcattgg aaattaaaga tgaagatttg gatatagata
    14521 gaaaattctt agaaagtcta aagagagaaa taagaagtct tggaaatata aatatagact
    14581 ctataaagga atatgaggaa ataaaagaaa gatacgattt ttatagcgaa caaaagcaag
    14641 atttagaaga gtctatggaa gagatagaaa aacttataca tactttagaa gaaaatatga
    14701 aatctgaatt tgaaattaag tttgaagaaa taagtaaaaa ttttaaatat gtatataaga
    14761 gattatttgg tggaggttgt ggagaattaa ctattttaga taaagaaaat ttattagaaa
    14821 gtgatatact aataacagct caacccccag gtaaaaaaat gaaaaattta aacctattat
    14881 ctggtggaga gaaagcttta acggcaataa gtatattatt tgctatattg attacaaagc
    14941 caacaccatt ttgtatatta gatgagattg aagcgccact tgatgacgca aatatattta
    15001 gatttggaga atttttgaag gatttatcta aagagactca atttatatca gtaactcata
    15061 ggagaggaac tatggaagct gctgattata tctacggagt tacaatgcaa gaaaaagcca
    15121 tttcaaaagt tattagttta aaattaaaag aagctcagga aataacagat attatataac
    15181 ttagattgac ataggaggaa taacagtgtt aaaaaaatta tttggttttg gaaaagacaa
    15241 ggaaaaggaa atagaaaaaa aagatgctga agaagaaata gaagttgaag acagtgttga
    15301 taatttagaa aacttggaag aaactatttt ttcaggactt gaagaagaag tgatagataa
    15361 agtggaagat gttgaagaaa aaaatgagga aagtgtcaat aatgaaggca ctgaagaaat
    15421 cgaaaacttt gaaaaaatag aagttgagat ggatagtaat ggaaaagaag tagaaaatat
    15481 ttcaaataat gattctcaag aagagataat cgaagaatta gaaaattatg atgaaattga
    15541 gtctaaagag atagaagata aagaagataa gaaagttaac ttgtttgaaa gactaaaaca
    15601 aggattgaca aaagcaaagc aaggaataac tgatagaata gatgaagttt taaaatcata
    15661 tacaaagata gatgaagaat tacttgaaga tttagaggaa attttaataa cagcagatgt
    15721 tggtgtaaat acaactatgg atataattga aagactaaga gataaaataa agcaaaaagg
    15781 tattactgaa ccaataaagg taagagaaga acttaaatct atagttgaag atattcttac
    15841 aaatgaaaac tctacactag atatagaacc agcaccatgt ataatattaa tggtgggtgt
    15901 aaatggggtc ggtaagacta ctacaatagg taaactagca aatagataca aaaaagatgg
    15961 taaaaaagtt ttattagcag cagcagatac atttagagca gcagcaacag agcaattaga
    16021 aatttgggca aatagaacca atgtagacat aataaaacat caagagggag cagaccctgg
    16081 agctgtagta tttgatgcta taaaagctgc aaaagctaga aaaacagatg tattaatatg
    16141 tgatacagca ggaagattac acaataaggc taatcttatg aatgaacttg gaaaagtatt
    16201 taaaatagta gatagagaat ttccagaagc aaaacgtgaa gtacttcttg tggtggatgc
    16261 tacaacaggt caaaatgcag ttgttcaagc aaagacattt aaagaagttg cagatataac
    16321 aggtatagta cttacaaaac ttgatggaac tgcaaaaggt ggagtagtcc ttgcagtaaa
    16381 atcagaagta gatgtacctg taaaacttat tggagttgga gaaagtgtag aagacctaca
    16441 agatttcaat gcaaaatcat tttcagatgc attatttggt aactaatgtt cttttaaaaa
    16501 taatggttga caagagtgtt gttatgatat aaaatacaat agtgtaaagt aaataacctt
    16561 tacagtatac tgctaggaag tgtaagatat gaatatagaa aaaatggtcg aaattggatt
    16621 gttatttgaa cagtataaag aattattaac tgacaagcag aaagaaatag ttgctctata
    16681 ctatgaagaa gattattctt taggtgaaat tagtgaaaat ctgaatgtat caagacaagg
    16741 tgtttatgat accttaaagc gttcagaaaa aatactaaga gattatgaag aaaagttaca
    16801 cttagtatca aagattcaag agcaggaaaa aaatattaag attataaaag ataaaattat
    16861 tgacattaaa gaagatttat tgcacaatag agattgtgct aatttaatcc ctaagctaga
    16921 aaatatagaa gatgtatgta gggagatgat aaaatgatat ttgaaggatt gtctgataaa
    16981 ctacaagggg catttagtaa gttaaaatct aaaggaaaac ttacagaagt agatgtaaaa
    17041 aatgctatga gagaagttaa acttgctctt cttgaggcag atgttaactt taaagtagtt
    17101 aaagatttca taaaaaaagt tcaagagaga tgtgttgggc aagaagttat gcaaagctta
    17161 actccagctc agcatgtaat caaaattgta aatgaagagc ttacaagttt aatgggagat
    17221 gtacaaagta aagtaatgat ttcctcaaag cctcctacaa ttttaatgat ggttggattg
    17281 caaggtgcag gtaagactac tacagcagga aaacttggag gatattttaa aaagcaaggt
    17341 aaaaaacctc tattagtagc ttgtgatatt tatagaccag cagctataaa gcaacttcaa
    17401 gttgttggag aaaaattgga tatacctgta ttcaatatgg gagataaaga aaatccagta
    17461 aatattgcga aagcaggttt aagccatgcg attaaaaatg ctaatgattt agttattata
    17521 gatacagctg gtagacttca tatagatgaa gtacttatgg atgagttaaa gtctattaaa
    17581 tcagaagtta agccacatga gatacttttg gttgtagatt ctatgacagg tcaagatgct
    17641 gtaaatgttg cagaaagctt caatgaggca cttggtgttg atggagtagt attaactaaa
    17701 ttggatggag atactagagg tggtgctgca ctttcaataa gagcagttac acaaaagcct
    17761 ataaaattta tggggatggg agaaaagcta gatgatatag aacctttcca cccagataga
    17821 atggcatcaa gaatattagg tatgggagat gtcttaagtt taatagaaaa agcacaagaa
    17881 aatatagact tagagaaagc taaagaatta gagcaaaaaa ttaaaaaaca agagcttgat
    17941 tttgaagatt tcttacaaca aatggaacaa atacaaaata tgggacctct tgacaagata
    18001 cttggtatga tacctggtat gggaaatgta aaagaccaac ttggagatgt agatttgaat
    18061 ggcaaagaaa tgaagagaac aaaagccata attcagtcaa tgactacata tgagagaaga
    18121 aacccaggag tattaaatgc ttcaagaaag aaaagaatag ctaaaggcag tggaactagt
    18181 gtacaagatg taaatagact tataaaacaa cttaatgaaa tgaaaaaaat gatgaagatg
    18241 tttgctggtt cacaaaagag tatgaagaaa aggggcggct taggcggttt accttttttt
    18301 aaataaaata taaaaaagta aaaattattc ggaggtgact gtaatggcag ttaaaataag
    18361 attaaaaaga atgggagcta acaaaaaacc tttctacaga atagtagtag ctgattcaag
    18421 agctccaaga gatggaaaat ttatagaaga aataggatac tataatccaa tttctgaacc
    18481 taaacaagta agaataaatg atgaaaaagc tataaaatgg ttagctactg gtgctcagcc
    18541 aacagaagta gttaaaaaat tacttgtaaa aaacggagta atagaaaaat tcgaagcttc
    18601 taaacaagca aaataatttt ttcttggcaa ttagtttaag taagggctca ttgctaaggt
    18661 tactttagga gatgaaatat atgaaagagc tagttgtaga tatagctaag gctctagttg
    18721 ataatcctga ctcagttgtt gttgaggaat ttgaagataa tgatggtatc atcttaaagc
    18781 ttacagttgc tcaagaggat atgggtaagg ttattgggaa gcagggaaga atagctaaag
    18841 ctataagaac tgtcgtaaga tctgttgcaa atagagaaaa tataaaagtt tctcttgaga
    18901 tagtataaaa taggaggatt aggtataatg cctagtcctt tttatataaa tgcgtagttt
    18961 aagattttat gatgatatga ccacataaga ttaaattttt taagaaggtg ataacatgaa
    19021 agaaaaatta actcatttta aggtaggtca aatagtaaat acacaaggtt taaaaggtga
    19081 agtaagagtt tatccgctta ctgatgatat agagaggttt gatgaattaa aagatttcta
    19141 tctgggaaaa gatttaaata ataaattggc tgttgaaaaa gtaagataca aaggttctat
    19201 ggttataatg aagataaaag gcatagacag catagagaaa gcagaaaaac taaaaaataa
    19261 atttatgtat gtatctagag aagatagcag agagcttgaa gaaggcgaat tttttatagc
    19321 agatatgatt ggaatggaag ttttaactgt agatggaaaa tatgtaggga tactagaaga
    19381 tgttcttcaa tactcagcaa atgatgtcta tgtagtaaaa ggtgaggaag ataaagaatt
    19441 tatgatacca gctataaaaa aatttgtacc tacaatagat attgatgaaa gaaaaatgat
    19501 tatagaccct attaagggaa tgatagatta ggtggtaata tgagatttca tataatgaca
    19561 ctttttccag aaatatttaa ctcgtacatg gatgaaagta taatgaagag agcagttgaa
    19621 aaaggtataa ttgaggttca tatatataat ataagggatt tttctaataa taaacataaa
    19681 aaagtagatg attatccatt tggaggagga gcggggatgg taatgactcc tcaacctata
    19741 tatgacacat ataagcatat aataacaact cataatataa ataacccaag tgtaatttat
    19801 cttactccaa aaggtaaggt atacaatcaa tctatggcaa agcagatgtc tttaaaagaa
    19861 gatataatac tactatgtgg tcattatgaa ggaatagatg agagaatcat tgatttaata
    19921 gttactgatg aaatttcaat tggagattat gtattaactg ggggagagct cccagcactt
    19981 ataatgatag attctatatc gagattaata ccaggtgtat taaaccaaga agaatcattt
    20041 gaagaagaat catttaaaga caacttattg gaatatcctc attacactag acctagagat
    20101 tttgaaggat taaaagtacc agaagtgctt ttatcaggga atcataagaa gatagatgag
    20161 tggagaagag aagaatctat aaggataacc aaagaaagac gatttgattt atataaaaag
    20221 tctaatgaaa aataaaaata taatgaaaaa ttaatatata tgtatacaat aaagtaccta
    20281 taaatggaat taaaaacata aaagtgcttg taataactat tgaagtatag tataatataa
    20341 aatgttgaaa atacgggcgt tcctctgttt acactggtcg ggtagttaag aacgtctatg
    20401 atatctagga ggaagaaaga tgaatgaaat gctaagagca attgagcaag aacaattaaa
    20461 aaatgaagtt cctaattttg gacctgggga cacagttaaa gtacacgtta gaattataga
    20521 aggaaaaaga gaaagaatac aagtattcga aggtgtagta ttaaagagac aaggtggagg
    20581 agcaagagaa actttcacag ttagaaaaat gtctttcaat gttggtgtag aaagaacttt
    20641 cccagttcac tctccaaaaa tagaaaaaat agaagttact agaaaaggta aagtaagaag
    20701 agctaaacta aactacttaa gaggtagagt aggtaaagct gctaagataa aagaagctag
    20761 aaataaataa ttaaaaactt tacttataaa tggggggctg atttatcagt cctctttcta
    20821 ttttaatgaa aattacagca aataattata ctagaaataa atagaaatat ttgttgaaaa
    20881 tatacattat ttatctatga atttatagct tttaaaatga aaaataaaat tggtaaatat
    20941 tgcattatat tttgtaacaa tattaaaaat aagagaacag tattatgtaa agtatgacgt
    21001 taaaaagatg acaatattat agtttgttaa gatagatttt ttaagtggaa ttaggatata
    21061 ataatataat attaataagg agtgatacat gtgtcaaatg aatacgaaga gtatcttatg
    21121 aatgacaatc tacatataaa ctggtatcca ggtcatatga aaaaaactaa ggagttagta
    21181 aaaaataact taaaattgat tgatgtagta attgaacttt tggatgctag aatcccgttt
    21241 agtagtaaaa atccagacat agacaggctt gtaggagata agccaagagt tgtcgtactt
    21301 aataagagtg atatggcaga tagggataaa ttgaatcaat ggattgaata ctataagaaa
    21361 attaatatca aagctatacc agtagataca ataaaaggcg ttggtataaa taagataatc
    21421 gaagaatgta aaaatgtaac tagagaaaaa atgagttctt taaaagacaa agggagaaaa
    21481 gaaagagcta tacgaataat gatagttgga gttccaaacg ttggtaaatc gtctcttata
    21541 aacaaattaa caggtagaaa aagcactcaa actggagata agccaggagt aacgaaaggg
    21601 aaacaatggg ttagattaaa gggaaatctt gaacttttag atacacctgg aatactttgg
    21661 cctaagtttg aagaccaaga agttgcatta aatttagcat ttagtagagc tataaaggat
    21721 gagattttag atactgaaac tttggcttta agacttatag agaaacttat gaaaatagaa
    21781 cctgagaagt tgaaagccag atataagtta gattgtctag gagaaactcc aattgaaact
    21841 atggatatga ttggccataa aagagggttt ataacaggta aaaaagaact agattataca
    21901 cgcatagcca caactgtatt aaatgaattt agagatggaa aaataggaaa tataacattg
    21961 gaagttccag aaaacgttaa aagatagatt ttaaaacaaa atcaaaaatt taatgttaaa
    22021 aatttaaaaa agccttttag caaaggcttt tttatttttt tgaaatatag acaaatatct
    22081 ccatataaaa gtataaatta ggttagaaaa cctttaaaaa tattaattta agacaaatat
    22141 acctaatatt ttgaaaatta aaatgagtgt gattatttaa aattttagca tattgacttg
    22201 cttgtttcgt ataatatata ataagcacaa ataaatacaa tatattaaaa atttaattta
    22261 tttaatttta ttaatttaat aataagttct tattgtttaa ttcaacttac ttatgcaagc
    22321 gtaaacaatc gagacatttt attaatttaa taaggttaaa aagggggatt ttataatggg
    22381 taagaccaag gatgttatag tatttggatt tgcgttattt gcaatgttct ttggagcagg
    22441 taacttaata tttccacctt acttgggaat aattacaggt ccagaatggt taattgcctt
    22501 cttaggattt acgtttgctg atgctggatt ggcacttcta gcagtaatgg ctacagctaa
    22561 attcgatgga aacgtagttg aaatgtttaa aagatgtggt atgaagttag ggattcttat
    22621 aggttgtgca gatattttat gtataggccc attcttagct ataccaagaa caggtgcaac
    22681 aacttatgaa atgggtataa tgcctttgtt tggaacttca attccagtgt tattattctg
    22741 tatacttttt tttgcgattt catatgtact aacaataaga ccttctaagg tcgtagatat
    22801 agttggacag tttttaactc cagctcttct aatagcttta gcatttataa ttataaaagg
    22861 tattatatca cctcttggag atatagtaga taaacctatg atacctaatg tatttgcaga
    22921 aggtataggt caaggatatc agacaatgga tgcttttgca gcaatagcac ttgcatcagt
    22981 gctaatagta tctttaaatg ataaaggata ttctactatt agtgacaagt tgaaaatgat
    23041 tggtaaggcg ggtgttcttg cttgtggagg attagctcta gtttatggag gattatgttt
    23101 ccttggggca actgtatcaa caatgtatgg aacagatgca gtacaatcac aagtaatagt
    23161 aaatataact gaaggattgc ttggaaatgt tgggaaagcg attttagcag tagtagtatc
    23221 attagcatgt ttaactacat ctataggtct tacatctgcc acaggtcaat atttctctag
    23281 acttacaaaa ggtaaattga gttatgaaaa aatagtatta gctgtatctg tattcagtgc
    23341 agtagtagca tcttttggtg ttggaactat aataaagata gcatctccta tactaagtat
    23401 agtttatcca ccatcaattg ttttaataat tttagctttc ttcaatgaaa aaattaagaa
    23461 tgataatgta tacaaaggag cagtatatat gtcattgctt gtaagtatac taacagttat
    23521 atctagttat ggagtagctg ttccagtagt aaattcttta cctcttaatt cattaggatt
    23581 taactgggtg gtgccagtta taatagctgg aataattggt aattttatac catcaaagag
    23641 tcaatctaat acacttggaa caaactaagt tttgtttaat taaaatataa tcaattgtaa
    23701 ataaatgagt taactaaaag taaaaggagt tat
//
LOCUS       DAFKMX010000044        23384 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFKMX010000044 DAFKMX010000000
VERSION     DAFKMX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.044"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            2..574
                     /locus_tag="LZB08_001401"
     CDS             2..574
                     /locus_tag="LZB08_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBM7314911.1"
                     /translation="MKKLVKILINIICIITLTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1585..2247)
                     /locus_tag="LZB08_001402"
     CDS             complement(1585..2247)
                     /locus_tag="LZB08_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7314912.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2264..4819)
                     /locus_tag="LZB08_001403"
     CDS             complement(2264..4819)
                     /locus_tag="LZB08_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7314913.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(5053..5247)
                     /locus_tag="LZB08_001404"
     CDS             complement(5053..5247)
                     /locus_tag="LZB08_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBM7314914.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSSDDIERITKEKYTKEN"
     gene            complement(5259..5438)
                     /locus_tag="LZB08_001405"
     CDS             complement(5259..5438)
                     /locus_tag="LZB08_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314915.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5443..5700)
                     /locus_tag="LZB08_001406"
     CDS             complement(5443..5700)
                     /locus_tag="LZB08_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314916.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQSSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5961..7354)
                     /locus_tag="LZB08_001407"
                     /pseudo
     CDS             complement(5961..7354)
                     /locus_tag="LZB08_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(7348..7593)
                     /locus_tag="LZB08_001408"
     CDS             complement(7348..7593)
                     /locus_tag="LZB08_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBM7314917.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKEGFLKFIGDRIC"
     gene            complement(7910..8062)
                     /locus_tag="LZB08_001409"
     CDS             complement(7910..8062)
                     /locus_tag="LZB08_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314918.1"
                     /translation="MNNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            complement(8846..10936)
                     /locus_tag="LZB08_001410"
     CDS             complement(8846..10936)
                     /locus_tag="LZB08_001410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314919.1"
                     /translation="MNKEYFSRKNTGGAINYIGTAFQDCVSLIYLFDNIDMEDYKEIT
                     FEQINDFTIIFKDYEVSVQVKSNYFDFKSVSTLLEKLEIKHGKKYVFVCSKYDKQFRA
                     FAIKLQQMKNVINSSRSNNEKEEIIEEIKNIAKKNGLNYEKAINVEFKELSLENRIDL
                     AKLKIYEWEKRNNLDIDVDALFDSLISNITQNLCTYRGFLSKNYINELALKYKSRKID
                     KNSTEYIKFVDNNVNSILEALKFDSSLYEMFTQQLNMTRFYISQNDFKNALKEIETIA
                     KYEQEFQKYKFFLLNINEDYKKLKNYCIEVLKNDESNYYANYYLGIEYKKIYKYKKAI
                     ACFLNAIDTMDTFDVNYNLAEVYEKSGQINNSLKYYNRCLEKDSMNIRANLNISKLLP
                     YNKAIEHLDIVISLDPEIYEAYLYKGKILRFLENIDEAFSNFQKYLKYREELEVIKEM
                     ALCMIDMNKKESNVFLSSWILESMQEELRELKDGESIIIYDILWNSSKVIICTKKEDD
                     FIVQSPISSFKLMNSNTNNLIVVGLVEDRFLKATSEFLRKNGRKAEKGKEYLPCFIKS
                     YDNKYDFDKFINSILEQKILHINKEYILNDEHGNEVEFREYISYESDVNIMMNDYNSH
                     RFVEVNIGSSTINGYFKKVGDGFFAFESKLEEGTPFNEGIIILECYEASKIIHIKMSL
                     DNIHSKKTLLYKYI"
     gene            complement(11298..12425)
                     /locus_tag="LZB08_001411"
     CDS             complement(11298..12425)
                     /locus_tag="LZB08_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBM7314920.1"
                     /translation="MTIPKLEPNDIKEEFNKYTSEQINKVVYNYLFKKNMSSKRIDEE
                     ILDLQFKQGKEYQSMEILNNLGINKKHKGIFIGCTIDKAIEVLKEDEIHFKQIINILL
                     ESKNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRHFFEINNLGKNDKK
                     NVNFRYDDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRNIFPKYYEKFLEGKIEENAN
                     LPKIRFAKLDSNNFDIEFIFTNEIEDDVLEEISQDNTLDVDTINVELETEIRKEGKVK
                     YVYGKQYERNPKNRIEAIKYHGTKCVVCGFDFEKIYGNRGKGYIEIHHIRPLSSVGKE
                     SNINPKTDLVPICSNCHRMIHRKKDSVLSIDKLKKIVKDSK"
     gene            complement(12699..12974)
                     /locus_tag="LZB08_001412"
     CDS             complement(12699..12974)
                     /locus_tag="LZB08_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314921.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEIMAINESLE
                     KSLLEENKIYVLMELNDLFNSLIEFERELSYKLGFKAGAKFIIELLK"
     gene            complement(13222..14556)
                     /locus_tag="LZB08_001413"
     CDS             complement(13222..14556)
                     /locus_tag="LZB08_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7314922.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEINEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKNIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(14569..15312)
                     /locus_tag="LZB08_001414"
     CDS             complement(14569..15312)
                     /locus_tag="LZB08_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314923.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITILLMASHKENYV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWEIYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIREYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKGTGTYNPDDSQLKW"
     gene            complement(15334..15648)
                     /locus_tag="LZB08_001415"
     CDS             complement(15334..15648)
                     /locus_tag="LZB08_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314924.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKVIENAKRNK
                     AELITCKEASRLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(15652..15852)
                     /locus_tag="LZB08_001416"
     CDS             complement(15652..15852)
                     /locus_tag="LZB08_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314925.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            15999..16763
                     /locus_tag="LZB08_001417"
     CDS             15999..16763
                     /locus_tag="LZB08_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314926.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDPTDTGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            17014..17505
                     /locus_tag="LZB08_001418"
     CDS             17014..17505
                     /locus_tag="LZB08_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7314927.1"
                     /translation="MMNNTIGEKIKQYRKSVNMSRDELANKISCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFNKEFDLVIHILENYDYDI
                     KTDYKTKFEITDNTDTIKPINLNFSDFKSFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            17594..18346
                     /locus_tag="LZB08_001419"
     CDS             17594..18346
                     /locus_tag="LZB08_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBM7314928.1"
                     /translation="MNKSYIPKYTYKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            18336..19502
                     /locus_tag="LZB08_001420"
     CDS             18336..19502
                     /locus_tag="LZB08_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBM7314929.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIELIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(19618..19848)
                     /locus_tag="LZB08_001421"
     CDS             complement(19618..19848)
                     /locus_tag="LZB08_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314930.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(19854..20330)
                     /locus_tag="LZB08_001422"
                     /pseudo
     CDS             complement(19854..20330)
                     /locus_tag="LZB08_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
     gene            complement(20330..20545)
                     /locus_tag="LZB08_001423"
     CDS             complement(20330..20545)
                     /locus_tag="LZB08_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBM7314931.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(20861..21210)
                     /gene="ssrA"
                     /locus_tag="LZB08_001424"
     tmRNA           complement(20861..21210)
                     /gene="ssrA"
                     /locus_tag="LZB08_001424"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(21368..21823)
                     /gene="smpB"
                     /locus_tag="LZB08_001425"
     CDS             complement(21368..21823)
                     /gene="smpB"
                     /locus_tag="LZB08_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBM7314932.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            22436..23263
                     /locus_tag="LZB08_001426"
     CDS             22436..23263
                     /locus_tag="LZB08_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBM7314933.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         8086 a         3472 c         2415 g         9411 t
ORIGIN      
        1 attgaaaaaa ctagtaaaaa tattaataaa tattatttgc attattacgc taactttctc
       61 tagcctttcg atttacataa aattatctga atataaaaaa gctgatgaag tatatacgga
      121 actcagagaa aacactatca ataatagcaa acatcaagaa ctttatgata aaaataatga
      181 ttatagattc tggcttaaaa taaacaacgt aaatatagac tatcctgtag tacaaggata
      241 taacaatgat ttctacctaa ctcatgattt ttataaaaat tatcttcctt taggtagtat
      301 tttcatggat tatagaaata attttgaaaa tgataagagt ttaatagtct atggtcatta
      361 tatgaaaaat aaaactatgt ttggtcagct agaaaactat actgatgaag tatttttcaa
      421 agaaaataac ttagtagaaa taaattataa agtacaaact tatacttatg aaattttttc
      481 agtatatact gctgacttaa ttaacagaga ttatttaagt atacacttta atagtaatta
      541 tgagtttaaa gattatttga attatataac ctaaaaatca gtacacaaat ctgatataaa
      601 ggtagatatt tcagataaaa ttataacttt gtatacttgt aactatgaat ttaaagatgc
      661 tcttactata gttcatgcta aattaatttc aaaaaaagag actgataagt aggtttgtgt
      721 aaatgcagac ttacctacag tcttttttga acgtaaaatt ggtaagtatg gaataatatt
      781 tatattaaac ttttgtaggc aaagaaatga aaaatcaaag agaaataatt attacagatt
      841 tagattacca agctgaatta agaaagtgta aaactatgga ggatgtagtt ggtgaagata
      901 gtatataata gtttaactta taaattatct tggtgatatt aatgaagttt caaagtatat
      961 attatttatt gatataaata aaattaaatc tgttgctaat agatgtacct ttgtttggag
     1021 acaaactatt gaagaaatat aactaaaatg ttgaagtagc tacctgactt tattttgaaa
     1081 tgcgaacaag attttggcat taagataatt tagaaagaat cactggtaca caaggatgtg
     1141 aacttagaat gaatagaagt attcaggcat aaggagcttt cacccaaata aaacatagta
     1201 tgaactttaa aagaattcta atcagagaca atcaaaatgt tctttcatag tttatacctg
     1261 tagtgttaga catagtattt ctaagtttca caaataagat aatcattaat aaatgtagta
     1321 tgtatattca tcctctcaaa aatatttttt aatcttacac tttgtaaatt attctatttt
     1381 aaaatggtat ttttatacct ttatttctta tgaccgtttt caagctcaaa ttttgcataa
     1441 atataaatta atgaaaagca agtaaaaaaa gaactgtcgc acagcaattt aaaattactc
     1501 attcgacagt ccttttttcg tggattaatg gtggagaaga atcacaagaa taatacccac
     1561 gattaaacct ttatacaata cattttatat gactttacca tcactaatag ttattataca
     1621 atcagccatc ttagctattt catcattatg agttatcata accaacgtct gcttgtattc
     1681 atttacacaa tactttagta aattcatcac ctcattagtt gttttactgt ctaaatttcc
     1741 agtaggttca tctgcaaaaa ttattgatgg cttattggca agtgatcttg ctattgcaac
     1801 tctttgttgt tgaccacctg ataattcatt aggaaattta ttaatctgac tttctaatcc
     1861 taacttagtt attaaatcat taatataatt attatctatc tttcctttag caattaaaat
     1921 aggaagaact atattatcat aaacattgat aacaggtatt agattatagt tttggaatat
     1981 aaacccaaat tctttacttc taagattaga tagcttttca tcacttaaag tatataaatc
     2041 taaattatcc ataaatacat tacctaaagt tggtttatca agccctgcca tacagtgaag
     2101 taatgtgctt tttccagaac cactttgtcc aattattgca ctaaatttat ggggttctat
     2161 ctctaaagta attccatcaa cagcctttat ttcattttct ccttttccat atattttttt
     2221 aatatctata acttttaaca tcttcataca tttacctccc aaattattca ttctctttta
     2281 taccttcaat aatattcata ctttcaaaat ctctattact tacaactact gctattaaac
     2341 atacaaatac agatatagca aagaatagta aagattcctt aattgggaat gtaaaagaca
     2401 taaaattctc tattccatag tttatatacg catcattatt ccaatttgta aatttaacta
     2461 gatttataaa tgatggaatc atagctatta taaagctaaa aactgcatat gctaatgctt
     2521 catatataag catttttttc atatttttaa gactaacacc taatgctcta agcgttgaaa
     2581 tttctttttt tctaattagt agatttgttc ttatagtaca gaaaatatta attgatgcta
     2641 ttattaaaac tagtgcaatt attgctattt gtgatttaat gtaactttta tatatacttt
     2701 ctgacatttc atgataactt aatctactat taaatagaga ccctggtata cttcttgaaa
     2761 ttttctgtat ctcattatta acaatctcat catatggatt ttctaaattt atgcctaatt
     2821 gagtgtattt ttgttctcct gttatgtttt tagagtgaat attacttgtt ataatttcta
     2881 gatctggcat aaaatcgcca tctcccatag caatccattc atcctttaaa attacagcca
     2941 ctcttacctt catctcttta taaacaattg tattattttc ttcaacagga atttttacag
     3001 ttattatgtc tcctatattt aattctttga atataggctt tatagtatga tcatcaacta
     3061 tatcataaaa ataattatat actgctacat ttggatatcc atcagtgtcc tttttaatat
     3121 catctaagct accttcttca acaaagtcat ttaacttttc atctatataa tcatcatcat
     3181 aaagctttaa aactaattta tcttccatta tctctttttt ttctactcca ttataggttg
     3241 taaattcttt gtttatatcc ttattattaa aagtaaacat tccatttgtt atttgaattt
     3301 tatgtatact tttaacaccc ttaatttttt ctatattctc tacatcacta tccgtgtatt
     3361 ttgatatttg cgggtctgca tttactccaa attgcattgt tatattatat ctattttgca
     3421 tatcttcaat tttactgttg acctcttctt gcatacttga gaatgtagta atgaatatat
     3481 atcctcctaa agcaatagac aacatagata tagcagctct aaccttattt cttccgatat
     3541 ttttaagtcc cataaatcca taaaatccaa atattttgct tactagtctg tctattccaa
     3601 gtctacttct gtgattttta attttgtctg tatttattat tgattcaata ggtgatatct
     3661 tgccgatttt aaaagttggt ataatacaag atataaaaac agaaattatt ccaaccaata
     3721 tagcttttat aactatttca ttatttatat ataatttagg gttaagacca gcttcactgt
     3781 ataaaacttc gttataatta ctaattccta tatatgaata tgtaacccca gctactaacc
     3841 caagtagtat acctattatt gttataatta atgcttgata gattatcata caccttacgt
     3901 ttcttttctt tgcgcccatc agtctaagca gtcccatttc ttttactgat tgatttagtg
     3961 taatattgaa aatattaaat atgaaaatta tagatactgt agttattaaa acatttcttt
     4021 taaaaacatt cggatcattc ttatcatctt ctacataacg aagttccctt acaagaggta
     4081 catttggtag aaagcttttt tgtcctaact tatatttttt cataagataa tcacattcac
     4141 cttcaatatt tctccatcca cttttaaaac ttaatatact attataacta atcgcatcat
     4201 gaggtataat gttatttgtt tcatcattgc cataagttac tgcttcgtat tgccaaacca
     4261 catcatagta cccttttggt ttttccataa ctcctactat tttaaattct ttatttatag
     4321 aaaataattg attacttccg ttagtatctt tatagttctt ttttattgtt aaatttaatg
     4381 tagatcctac tccttcactt aaattcattg cttctagtgc ttttttctct aatactattt
     4441 cattgttgtt tttaggtagt ctaccttcta ttattttgtt gtatctactt tttagatact
     4501 cttcgtttga tgaagttaat ataaatgagt ttcctttttt atctgaaata cgtcctagat
     4561 tttgtaccga attacccttt gatacttttt catcagtttc taatttttta gcatcatcaa
     4621 gtgtcaaatc actaaactct acatggtatc cactgtaaag ttcatgtatt gctactacat
     4681 tgttttcatt ttgtgaatct tttataacac taggtccaaa tatcaacata acacctaaca
     4741 taactcctat tattaaaagt aatgttctaa gtttattttt ctttaaatat gctagtgaca
     4801 tttttaagta aatccccata taatcctcct attttactat aaattattta tccaactcat
     4861 ctgttaagta gattggtgga tattttaaat cttttaattc ttcattattt tttgaagctt
     4921 actaccatct tcctcatgat aaacttgcag tatcacctta tttacatgta gtgcataata
     4981 atcaccatta taaaaatatt agtatttata acctggatag tttttgtaaa atgatttata
     5041 aagtatccca tcctaattct cttttgtata cttttctttt gtaatacgct ctatatcatc
     5101 acttgaaact ccttgatatg tcatatattt atctatatat ttttcagctt cattccattt
     5161 tctaaaatag ttgttgtata taataaatcc tattatggct ataatgctta taattaaagt
     5221 taataaaata gctttgtatt ttctcatagt gaatcttcct acttcaattt aaattcttta
     5281 ataagtttta attctaaaga cttagtttta tcatcaaatt tcttaacttg aatttttata
     5341 tctcccaaat tatcaacctc tacatttcct tcacctttat caaatacttc ttcaattttt
     5401 ctaaaaaatt ctttctcact tatttctaca tgaatcaatg atctatatgt tataaatagc
     5461 cattatgaat ttatcatctt ctgacagttt ttcattataa gaaaaatctt taacacattg
     5521 aattgataat atttcctttg tacccttctt gtccttagtc attgaataat gtaaaacttg
     5581 gctagattgt tcttttgtat actccaagtt tcccaaagac aaaacaaaac ttccatctga
     5641 tttattttca gctaccctaa gaccactatt ttctattgat tctgatattt tctttgacaa
     5701 gtcttcttga tactagaaat ctgttcaata gtatatattt tttcatttgt atctgaaatc
     5761 ttatttgtgc atcctgttat taaacctagt gttaagctta tagctataac catagttatt
     5821 gtaatttttt tatcttcata taatcctcct tatatattga aaactcgtta tttctcataa
     5881 aaaaaaagac gctatctttt tctattaaaa gataacatcc ctatatgttt agtatcaata
     5941 tatgttcccc aactggtagg ttaatcgacc caactatcag tattttgatt taatggtata
     6001 taaatattca atataaattt gtctttttta tcttcaaata ataaatctcc tctatatttt
     6061 tttatagaag atttgatgct ttgaatccct attccatgta caagtttatc ttttttatct
     6121 gttaataata aacttttatt ctttctaatt gaatttattt tactattctc acatttaatt
     6181 acgaaatagg actttgagat agttcctttt atttgtatat atttatcaat acttttatca
     6241 tgtatcttgt cgcaagcttc tattgcatta tctaagtatt tgcaaatata ttagatacat
     6301 ctattggttt tataaaattc actttggaaa aattgatatc acaaatgaat ttaatttttc
     6361 ttttttcact tacatcactt ttttcattta ttattatatc caatataaca ttacctgtat
     6421 tgtaaattga tttaaattct ttaatttaat cttttaaact atttacatac ccatcaattt
     6481 tcctttttta tatttgagat tttctataca atatatatga ttatttatat catgataaag
     6541 cttcttaact ttagcgtgtg attcttgaat acttagataa tgagaatact gcatatctaa
     6601 cttatttttt attacttcat tttgcaattt taacttattt tccttaatta tttttataaa
     6661 cactataata atgactaagc tactagctag tattaaccta ggtatcacaa tatacccaac
     6721 tatatatatt tcctaatacc caccaaatag atacattttt ctatttatat aaattattaa
     6781 tatagtaaat aaatttgtta ttaaagcaac agataagtat atatagttta ttttattatc
     6841 tttatctagt cccaatcttt tattaataaa tttaaatata acacaagcta ctatataatt
     6901 attattataa gataatagta tatatctact ttagcattat cataactaaa cactaataga
     6961 gcttcctctg atgatattac attacttgta actacaaaaa ataaatttat agatataaca
     7021 tcttttatta aagggtgata tacgtcaaat aagaaaaatg aaactatata tttaacaatt
     7081 cctatcttat aaaaataatt atagtaagtt cccaaataat aaatatcaaa taatagatta
     7141 tatcatatag atttttcttc atattataat tatgggtctt atcaacgtta aataatataa
     7201 tgattttagt tataagataa gctaataata cagtaaaaaa agatattttc ttagtcttat
     7261 ctgtatcttt atcgtaagtt aatatacttt ttagtatcat tattggtgta aacaatgata
     7321 atattaataa tattttattt atatggttta acatattcta tcaccaataa attttagaaa
     7381 cccctctttt atatctttat atctatatct actaataggt acttcttctt gactgtctaa
     7441 tattgcagta tttgacttta tattttctac atagtttaag ttaattatat aacttttatg
     7501 acatcttaca agttcctcta tatttaaatc tttttctatt ttttctagta tacatatagc
     7561 atcaaaagtt ttattttttg tatgtattag catgttcttt ttttgaactt ctatctattt
     7621 ttttgctaga gtagattttg tatgtgttag acttattttt atttttttca atttttttga
     7681 tacatgttaa tatgtgtttt tctagttctt gaaaatttat tggctttaat aagtatcaat
     7741 aagctcttac ttcatatctt tcctgtacat aatttattag agatgttata aatattatta
     7801 tctacttttc ttatgtttct tgcgatatcg atactattta gttctggcat ttttatatct
     7861 aagagtagtg tatcctctca attaactttg taatggttct tcttttgagc tatatttttt
     7921 taattcgata ttctttgttt ttatttttta atatattatc aacttgattt tttattacga
     7981 tttgtatttc tttttcatca tcacaagctg ctattgtaat gatgacctct cctagtattg
     8041 tatttttatt tagattattc actttaggtt aacacttttt aaatgtattt atcaataata
     8101 tttaaattaa aaatgtataa aaatacctat atccaattgg atataggtat tcgtggattt
     8161 caatggtgga tacgagggga gtctataagt ggttatttga ttgttttgtt ggtttttgta
     8221 aagctgttta tacaaatatt tacatggaat ttcatatatt tttttatggt ttttctaagt
     8281 atttttttct acagttgggg tataattggg ggatttataa ctataattaa atatatctta
     8341 taatgaatcc aactagttgt tcattaaaat ttcagcacta tttacccact tcatttattc
     8401 gttatatcct aatcataact ttatcttgat gacaatattc acttatttcc tttaattaca
     8461 tttatagctt taacaaatct atttaattct tcaatactaa ttttaaatat atctcttaac
     8521 tatataatta atcataaaac aaaatatatt tgtttagtat taaaatactt attaaatcct
     8581 ctactttgat ataaatgatt taaaatttca atattaatat ttaataactt ctttataaca
     8641 tcatttctat aatttatctt tttctctcat tttaacacat aacttaaaga aatcttaaat
     8701 caactaactc taagcaataa atacatcctg aaagataatt gactaaaata aaatatttat
     8761 tgtatcacta ccttttacat atatttaatt tgcttataca atattattac acttttattt
     8821 gcaataaaaa ccctaaccca ctacatcaaa tatatttata taataatgtt tttttactat
     8881 gtatattatc tagactcatt tttatatgta taattttact tgcttcataa cattcaagta
     8941 taattatccc ttcattaaat ggagtccctt cttctaattt actttcaaag gcaaaaaatc
     9001 catctcctac ttttttaaaa taaccattta tagtagaact tcctatatta acctctacaa
     9061 atctatgact attataatca ttcatcatta tatttacatc tgattcatag gatatatatt
     9121 ctctaaactc aacttcattt ccatgttcat cattcaatat atattcctta tttatatgta
     9181 atatcttttg ttctagaatt gaatttataa atttatcaaa atcatactta ttatcataac
     9241 tcttgataaa acaagggaga tactctttac ctttttctgc ttttctacca ttttttctta
     9301 aaaactctga tgtagcttta agaaaacgat cctcaacaag ccccactaca attaaattgt
     9361 tggtattact attcataagt ttaaacgatg aaataggtga ctgtacgatg aaatcgtctt
     9421 ctttttttgt acatattatc acttttgaac tattccataa aatatcataa ataataatag
     9481 attctccatc tttaagttct ctaagttctt cttgcattga ttctaatatc catgaactta
     9541 aaaatacatt tgattctttc ttattcatgt ctatcataca taatgccatt tctttaatca
     9601 cttctaattc ttctctatat tttagatatt tttgaaaatt tgaaaatgct tcatctatat
     9661 tttctaaaaa tctcaaaatt tttcctttat ataaatatgc ttcataaatc tctggatcta
     9721 aacttatgac tatatctaaa tgttcaatcg ctttattata tggaagcaat ttactaatat
     9781 ttaaattagc tcttatattc atgctatctt tctctaaaca cctattataa tattttaaac
     9841 tattattaat ttgaccactt ttttcatata cttctgcaag attataattt acatcaaatg
     9901 tgtccatagt atctattgca tttaaaaaac atgctatagc ctttttatac ttatatattt
     9961 tcttatattc tattcctaag taataattag cataataatt actttcatca ttttttaaaa
    10021 cctctataca gtagtttttt aattttttat aatcttcatt aatatttaat agaaaaaatt
    10081 tatacttttg aaactcttgt tcatattttg ctatagtttc tatttctttc aatgcatttt
    10141 taaagtcatt ttgagatata taaaacctag tcatatttaa ttgttgtgtg aacatttcat
    10201 ataagctaga atcaaatttt aatgcttcta aaattgaatt tacattatta tctacaaatt
    10261 taatatattc tgtagaattc ttatctattt ttcttgattt atattttaat gctagttcat
    10321 ttatataatt tttacttaaa aaacctctat atgtacatag attttgtgta atattagata
    10381 ttaatgaatc aaataaagca tctacatcta tatctaggtt atttcttttt tcccattcat
    10441 atatttttag ttttgccaaa tctattctat tttctaaaga taactcttta aactctacat
    10501 tgatagcttt ttcatagttt aatccatttt ttttagctat attttttatt tcttctatga
    10561 tttcttcttt ttcattattt gatctagaag aatttataac atttttcatt tgttgtaatt
    10621 ttatagcaaa tgctcgaaat tgtttatcat atttagaaca tacaaataca tattttttac
    10681 catgttttat ctccaacttt tctaataatg tacttactga tttaaaatca aaataatttg
    10741 acttcacctg tacagatact tcataatcct taaaaataat agtaaaatca tttatttgtt
    10801 caaaagttat ctctttataa tcttccatat ctatattatc aaataaataa attagactta
    10861 cacaatcttg aaatgcagtt cctatataat taattgcacc acctgtattt tttctactaa
    10921 aatattcttt attcatattt ttactcctcc ttaattatta acaataaatt taactaattc
    10981 ctattcaatt aattattata aaaacttata tccctgaaaa tacaaatttt catatatctt
    11041 aatactaata attagtagaa ttctataagt aatcttttta tataattatc attttagcac
    11101 taattgaatc atttctcagg tatatcgtta aatctatcat aatattaaaa ttacttttat
    11161 attgtctaat gtattttcaa ttataaaaaa gatatcttta aaagctatta tcatcaaaaa
    11221 ttaaactttt ttatgttaat atttattaac acttaactta aaaataaatt aaatactata
    11281 ttaatattaa ttaagtatta tttactatct tttacaattt tcttcaactt atctatactt
    11341 aaaacactat cttttttcct gtgaatcatt ctatgacaat ttgagcatat tggcactaaa
    11401 tctgttttgg gatttatatt actttctttt ccaacagaac ttaatggtct aatatgatga
    11461 atttctatgt atcccttacc tctgtttcca tatatttttt cgaaatcaaa cccacataca
    11521 acacactttg taccatgata ttttattgcc tctattctat tcttagggtt acgctcatat
    11581 tgtttcccat aaacatattt aactttccct tcttttctga tttcagtttc caattctaca
    11641 ttaatagtat caacatctaa agtattatct tgacttattt cctctaatac atcatcttct
    11701 atttcattag taaaaataaa ttctatatca aaattattac tatcaagttt tgcaaatctt
    11761 attttaggta aatttgcatt ttcttctatt ttaccttcta aaaatttctc ataatactta
    11821 ggaaatatat ttcttagcaa atctctaaac ttagatttcc aaattattct acttcttgga
    11881 ttctctaatt tatccatctc tataactgca ctataataat tatcatcata tctaaaattc
    11941 acatttttct tgtcgttctt ccctaaatta tttatttcaa agaaatgtct tatttctatt
    12001 ggaacccctg tcccattatg ttttaatgta gatttatcaa ttgtttttaa agctattttc
    12061 tcatctaata actcccaaga gtcaactatt ataggatact catttatatt tttactttct
    12121 aaaagtatat taataatttg cttgaaatgt atctcatctt ctttaagcac ttcaattgct
    12181 ttatctattg tgcatcctat aaaaatacct ttatgttttt tatttatacc taaattattt
    12241 aatatctcca ttgactgata ctctttacct tgtttaaatt gtaaatctag tatctcttca
    12301 tctattcttt tactactcat atttttctta aataaataat tgtaaactac tttatttatt
    12361 tgctctgatg tatatttatt aaactcttct tttatatcat taggctctag cttaggtatt
    12421 gtcatattcc cctccttgat tgattatttt agattttaat aaaattattg tatcatatat
    12481 ttattataaa aacatattgt gttattctat taaatacaca aatactgcaa cataattaat
    12541 tgcataaaaa attaaagagg gcatccttat accctcttta tgaaatatat ttattttaaa
    12601 caatataatt aaaacacact taattcaaag tattatagta caacttatta ataatctcag
    12661 tttgctattt aattgttcta ctgaaataca caaattaatt acttcaacag ctcgattata
    12721 aattttgctc ctgctttaaa gcctaattta tagctaagtt ctctttcaaa ttctatcaat
    12781 gaattaaata aatcatttag ttccatcaat acatatattt tattttcttc taataaactt
    12841 ttttctaatg attcattaat cgccataatt tctttccaac tttcaatata atatttgtct
    12901 ttttctaaaa tatctctata catatgttca tttatccaat aatcaaaata tatttctata
    12961 atttcatcat acattttagt acctccttga ctttcatcag gcacttaata tatacttaag
    13021 ttgctaggtt agtgtacatt aagtacctaa tttataaagg tagttgttat atttatatgg
    13081 caactacctt tttctaataa gcttttaaat ctaaactcaa tatattttaa atctctattg
    13141 tatctaatta aaatttatat ttatccttca atacttcttg aaacataatt tatctattac
    13201 ttaataaatc atgcttcttt tttatatctc ttgtatcttc ttacattctt caaaagaaaa
    13261 caaatttcct atataccaat atggtctaaa tcctgactca ccatccctat ttttagcaac
    13321 aataatctca cttaaattag caccaaaacc tttttctctt cgtctcagct ctcttatatc
    13381 ctcaattccc attgtcttac aataattaag agcttctttt tctgtaggtc tatgaatata
    13441 tataaccgaa ttagaatcca tatatatctg ctttgagctt ctcattactc tatccccttt
    13501 gggtctttca tctttcaatt catcatttaa ttgagcaagc tgaataactg gtatattaaa
    13561 atctaaagta atctttttta tttgccttga taagtcagcg aatttagctt ctcctacttc
    13621 tttaccttca taatccaaaa tttgcaaata atcaattatt actaaactag gcttaatatt
    13681 ctttatttct ctctttattc ctgatactgt gtttacagag tcatttatat aaagattatt
    13741 ttcttgattt attatgctta aagcttgtat atatttgact atttcatcat cgtttagatt
    13801 tctatttttc atctttacaa ctgatattcc agttagtgat gctatatttc taaacaaaat
    13861 ctgtatatcg ctcatttctc cacttataaa aagtactttc ttaccttgaa atattacatt
    13921 tctaagtatt tgtaacgcca atgctgtttt acctcttcca cttttagctg ctattgtagt
    13981 tagttcaccc ccatataaac ctcctatagt atcatctaag aactttattc cagttttaat
    14041 accttcattt atttcatcat ttatatattc aaatgctctc tctgatatgg ctgttatact
    14101 tgaatccttt tttattaatt tctgattagc tttatttata tcattttcta actgatatat
    14161 aagagtatta tcatctattt ctccaagatt gaacttattg aatttatcaa ttatatttct
    14221 atttttgtat ttttcaataa ctatacttac ataactcatg aaatttgata ctgttggaac
    14281 tatggttgac aaactagtga tatagcttat acctccaata ttattcaaat gaccttttct
    14341 ctttaattct tctgtaagag ttattatatc tattacttca tttctcttat ttatttcttt
    14401 tatagcatta aatatgtatt tatgttcctc atgataaaac atgtcttcat ttaacatttg
    14461 ctctaactct tgtactattg ttgaatctaa taaaatactt cctaatattg attgctctgc
    14521 ttctgtattt ttaattaagt tgtttgaatt atttataata taacctcctt accactttaa
    14581 ttgtgagtca tctggattat atgttcctgt ccctttattt atgacaattt ccttgctatg
    14641 ataattgccc tctagtatct tggtaaaatt acttgaattc attatccaat caaaatttgc
    14701 tttccatcct gtattattat ttccattcaa aaaacttgtt ttgctaacta tatttataac
    14761 ctctaataca gtttctttat catattctct tattcttgaa tttattctcc tctttcttgt
    14821 gtctgttagt ttcttgacct ttgaaagttc aatacaagtt gagttaaatt tgcttataat
    14881 ttcactatat ataatattat tttcattctt tatattcttg attgttgttg attgattgtt
    14941 aatcgtctgt tgattaggtt gttgattttc tatgttctct tggtaaatct cccagttttc
    15001 aatagttatg actatatatt tgtttgttgt ttcgtatgtt agaaaaccag catttttaaa
    15061 aaccaacaaa caacttctaa ccatttgata actaactcct ttaccacatc tttcagctat
    15121 cttgctatag gaagaaataa attgtccaga ctttattttt atccttttgt tatctataaa
    15181 aacatagttt tctttatgac tagccataag taatatagtt attaatatta cttttctttc
    15241 tggtttaatg tctttccaaa ttgatttatc caacaactcc ctataaagct ttatccaccc
    15301 tttattactc aattacaaac acctacttta agactataac ttgataccat ttgcattttc
    15361 aataaaccaa tcatccaatt tattttttat tatgtatact cgattattta tccatataca
    15421 agggaatcct ttatttttag aatgacataa ctgcctaaat tttgattctc ctataggata
    15481 aagtctcgat gcttccttac atgttataag ttcagcttta tttcttttcg cattttcaat
    15541 aactttatat ccttgctttt tttcttgtgc ttcttcttca ttctttacaa taatgtttaa
    15601 agtagcttgt aatgtcttta aaacttctaa attattattc tctttcatac tttattcctc
    15661 cttactaaaa aataattctt ctactgttgt atctaaagct ctagatagtt taatcatcaa
    15721 atcattgctt ggattattag ccttacctgt ttccaagttt gacaaataat atcttgatat
    15781 accaactcta tctgctagtt cttgttgctt tagacctttt ttaatcctaa atatttttaa
    15841 attgattccc atactttttc ctcctttttt ctagtttaca ttcgtaaact ttacatttta
    15901 attataatgt cgacatatgt atactgtcaa taaaaatata ttatttttct gttctcattt
    15961 gtgtactttt gggctatact ttatacgagg tgataatatt gcaaattgga aaaaacttaa
    16021 aaaatataag aaaacaaaaa gaactaacac agattcaact tgctgaaatt agtggcatct
    16081 caagaaatgc tctaataaat tatgaaaatg ataaaagaat ccctagtata gataccttat
    16141 caaaactcgc taaagctttg aaaattgaaa agactatctt ttattttgat attgaaaatg
    16201 aagtaatgac taatgaagat gttttatact ttttacagtc tttattccca gaagaatact
    16261 attcttttga tgaattttta aatgctattt caaatcttat ggaagatgat gatattagaa
    16321 ttacctcaag tttattgcta ggtgcttggt ctgataaaga ccaatattat aaacttggtg
    16381 aaaaattaga actaaatgaa aatcaagttt ataattggat actttcagat tgtatatcta
    16441 atactgaatt agatttttca ttacatcctg atgacataaa agaaattatt aaaaatgatg
    16501 ttcttacaaa acaatcaatt gatgaattat tagatattgg tctatcagaa gaaaatatag
    16561 aaacaatcta tgattatttt agtgacccaa cagatactgg gagtaatatt cgactaaaaa
    16621 aattaatatc tcaaaaagaa aatattacta ttaaaaataa tgattacttt aaattattac
    16681 aagaaaataa cgaattaaag gaaaaaaatg aaaagcttga aaatacactt aaagaaataa
    16741 aaaaactact aactaaaata taaaataata tcaatactat ctaaagcaat gataaaaaac
    16801 tatgaatgga gttacaatta tgaataaatc atatattcta aaatacacat ataaatgccc
    16861 tactaattta tttcatggag ataaaaaata tactctaata tacattaaaa gaattatgag
    16921 taaattagta tatgatatgg ataatctaga aaataactcc tttacatgtt tagaagtaca
    16981 atcattgttt gataatatat ttaaggtgaa attatgatga ataatacaat cggagaaaaa
    17041 attaaacaat atagaaaatc ggtaaatatg tcaagggatg agctagccaa caagattagt
    17101 tgttcagttc atgctatagc taaatatgag caaaatcaaa gaatgcctag cctagatatg
    17161 ataagaaaaa tagctactgc tttatcaatt gctataaata aactcattgt tgaagaaaat
    17221 gatttagata ctttaacaaa taataatatt aatttaaaag tatttaataa agagtttgat
    17281 ttagtaattc acatattaga aaattatgat tatgatataa aaacagacta taaaacaaag
    17341 tttgaaatta cagataatac agatacaata aaaccaatta acctaaactt ctctgatttc
    17401 aaaagcttct ctaaaaatct aaattgggtg ataaatggtc ttgttgaaaa ttttatacaa
    17461 gaaaactcca atggtcttat tgaagatgaa gatgaagaat attagtattg cacattttca
    17521 aaatatatat ttacttatta attttgatat agtcataatt agataagaat taaataagga
    17581 aggagctaca atcatgaata aatcatatat accaaaatac acatataaat tccctactaa
    17641 tctacctcat ggagataaaa aatatacttt aacatacatt agaagaatga taagtgaatt
    17701 tgtatatgat atgggtaacc tagaaaataa ccccttcaca ttcccagaag tacaaacatt
    17761 acttgatggt ataacagtag gtggacataa attaagtgac caaaatcaaa tactaaatat
    17821 aaaatcatca tgggattatg taataaaatt aagtaacaaa gaaaacttat ctttaaataa
    17881 agatacaatt tgctctatac ataaaatagt tgcaaaagat gaagctctaa tcgttggaga
    17941 tttcagaaat ggtaacatag gtatagctgg tactactcaa tatgaatgca tagaagccac
    18001 tctattaaat gatttatttg taagtgacat aaacatcata aataaaataa ctaacccctt
    18061 agaaaaggct ataataataa atttatggtt gtcctattgt caattcttct atgatggcaa
    18121 caaaagaact gctagactta cctcaaacct aatacttata agtaatgata taggagttct
    18181 atcaatacca gctaaacata aacaagaata caacacatta atgctaaact tctatgaaac
    18241 actagaagct gatgaagtta taaaattctt attagagaaa tgtattacat tctttgatgg
    18301 atataactat aaatcatata aggagctgtt taaaaatggc aactaaaaga gctaatggag
    18361 aaggttctat cggaaaatat aaagatggtt ggcgttcccg tataatgatt ggctataatg
    18421 aagatggtaa accaataaga aaagaatttt atggtaagac tcaaaaggaa gttaaagata
    18481 aactagatgc ttataaaaaa caatattatt taagctctga tatttcagat gataaaataa
    18541 ctttagagca ttggttttac aattggttgt ttgaatatag aataaaagat ttaaaaccta
    18601 agtcatttga aagatatgaa agcatctatc gtaattatat taaagatact gatttaggca
    18661 atgttaaatt aaaggactta agagtatctc acatgcaaaa atattacaat agattgcttg
    18721 attccaataa aagtatacct actataaggc aaataaacac taaactgaaa acctgcttat
    18781 cagaagctga aaaacaaggt cttatacaac gtaactattg tactatggta aatataccag
    18841 ttgagaaaaa ggaaaataag cttgaaatat tgactcttga acaacaaaag agttttataa
    18901 aagctataga tggacataaa ttagaggtat tatttttact tgctcttggt actggactta
    18961 gattaggaga attattaggg cttaaatggt ttgatattga ttttaaaaag tctaatttaa
    19021 cagttaaaag aactcttcaa agaacatatt ttatagataa aactggaaat agagagctta
    19081 aagtattaga gcaagaacca aagacatcaa attcttacag aactgtacct attccaaaag
    19141 acgttttaaa taaattgaaa gagcataaag taaatcaaaa tacagatata ttaaaggctg
    19201 gagaattata tagaaatgat aattatgtat tttgcaatga attaggtatc cctatagatg
    19261 ataaaagacc tttaaggaat ctaaagtcta tattaaacag tttagatata gagcttataa
    19321 agtttcatgg tcttagaaaa acttatgcta ctagactttt tgagaatgat gtccctccta
    19381 aaactgtaca agttttaatg ggccattatg atatatcaat aactttaaat atatatactc
    19441 aagttatgga agataagaag gttgaagctg tagaaaagtt ggataaaata ttttcattgt
    19501 aaaaatcaaa tacatataaa tattgtgatt tcatatagtt aattacaaaa tcaaaataag
    19561 gatacaaatt aattttatat tatcttgtat ccttatttcg tatctagttt taattattta
    19621 tcttggatag cctttatggt acattaatcc attttctaac attaattctt gagccaagta
    19681 tatatgaccc ttacagtgca tatctgccac accttcactt ataagtttat atgtttcaga
    19741 agtatataaa taatccatac tttcttccaa gctcttacct gtttgcttag aaaattctaa
    19801 tatgattttt ttatatagta tttgtaataa tgttttattt gcttccataa tcactatacc
    19861 tcctcttcac atctaaactt taaatattta tctattacat tttgattggt tatacatatt
    19921 tgttcatttg gtttatagta ttttaatctt tttaaagctt catctttttc aataagatta
    19981 tcttgataca gttctattgt attgtaaatc ttattatcag caatgccact attataatgt
    20041 catatttcat atatattact catatttcta cattctatta taaaatcaag ccattctaaa
    20101 ttataactat tgaagatttt taccttgcag ttttctttta atttatcaat ttctaaagaa
    20161 taactattta aatatgcttt tttacctctt tttttaaaac gctctgtcca gttaattgct
    20221 tgtgtcttta tactggttac atagaatcct tttccaaaat ctaaagaatc tcttgaaaag
    20281 gtaatactag gcgtctttac cacacaatat gaaccatgat atacaatcat tatatcacac
    20341 ccttttcttt aagtacttgc attaaatctt ccactatgta acctttactt tgagaatgta
    20401 gtgcttcata gcaaggtatt atatagttat ctaaaatatc agtatcatgt ttaagaagtt
    20461 tataaactcc ttctgaatct atattcaatt tttctgctaa agcttcaatg caaaaaatag
    20521 aaaattctag ttttctttca tccattacaa acctcaactc ctatcaagct tatataatat
    20581 atatttttac taattaacaa cttaaactgt cttacattta taaaaatatt atatcataaa
    20641 gtagatatta aaattctatg ttacctatta tcataataaa caaataaaat aataaacaaa
    20701 taaaaacttt ttatacagcc taaatgcacc ccaatatacc cccaaaaaaa cctcattttc
    20761 tgtggttggg gtacggttgg tgaatttctt ctccaaaata aaaaatacta gtcaacaaag
    20821 actagtattt ccaagtattt catcattctt ttttcaaaat tggtggagac gaggggagtc
    20881 gaacccctgt ccgcaaatct tttccttgct gcatctacat gcttagccaa cttcactaat
    20941 atctcacctc acatagcgcc aagtggcagg ctctatgctt agccagtccc aattaatcta
    21001 cttcaagtcg agacacccct aaagtagttc cctgcataat gacgtccagt cttagtcggc
    21061 aggaggacta agagggacga gcagcaacta ggctgctatc gcgaaattat cgtctgcgtt
    21121 tattttaaag ttccccagtt tttacgtgac ccagagtaac acgacatgca gcctcaagtt
    21181 ccaaacccac gtcgaaacct ttacgccccc ttgagtagtt tatagtccag tagacttttc
    21241 gcaaacttta tcaccatttt tattaaattt ttaatgtata tataatataa tacttttaag
    21301 aataaaaatc aatgttatgt tctagataat aaagaatttt attctaaaaa atacagtttt
    21361 atctagttta atacttacct gtcatttctc tttctatacg tctttgtgca tctttcttag
    21421 ccatatcttg acgtttatca tatagttttt taccttttgc taaggcaatc tctactttaa
    21481 ctttaccgtt ttttagatac aaactaagtg gtataagtga ataccccttt atagtagttg
    21541 ctccaataag ttttcttatt tcatgcttat gaagtaatag ttttctaact cttaaagaat
    21601 caacattgaa tatatttcct tgctcatatg gacttatatg aacttgtttt aaaaatactt
    21661 cgctattatc tacagatgca aatccatcac ttaggttaac tttgccttgt cttattgatt
    21721 ttacttctgt tccttttaat tcaattccac attcataagt ttcttctata aaatattcat
    21781 gtcttgctct tttgtttgtt gctaatattt ttttaccagc catataaatc tcaccttctt
    21841 tgctagaaat ttgttccata aatgttagaa atttactaaa tatttaatgt tgccaaatta
    21901 tttgactaac ttgtatgata acatatttag gcattttagt caatatttca ctgatatatt
    21961 aagttagtta ggctatatat aattaagtaa tataaatgta tgaaaatagc taaaaaacta
    22021 cttcgaatat acttaatact aaactaactc taaccagtaa tactaattta taaatttcaa
    22081 aacaaaaata ttacatatta taaatcttct aacttctaac ttctaacttc taacttctaa
    22141 cttctaactt ctaacttcta acttctaact tctaacttct aacttctaac ttctaactaa
    22201 ttttaacatg cccataaaat ataataccca caaatgataa ataatactca aaatatctat
    22261 tgaaataact atacatcctt aaataactta acttaaatct aaacatttca aaaacttctt
    22321 aaaaatatgt aaaaatttga tgataaagta gcgatttttt caaaaaaaat gtaatataga
    22381 gttatatctt ataatatttt agagataaat tttatcatag tggggagaaa tttgtatgga
    22441 tttaagcaaa aaagtaaaaa ttttagaaaa tgcatcagat ggcaaaagcg aatttgagat
    22501 attagaattt gaaaaacttg agggagcttc tagcccatta atggcaatgg gattatattt
    22561 tgctagagaa gctggtttga aagctagaca aattaagata aaattaaata attcatcaat
    22621 aaaaacagaa gctggtgcat tatactatta taaaggaaat atatcttctg aaactaagat
    22681 tggtggtgtt ggaggtcttt ttaaaaaagg aatatcagga gcacttacta atgaatcaat
    22741 gatgaaacct gtatacaaag gtactggaga agtttatttg gaaccttctt ttagacatta
    22801 tttatttatg agtcttgata atgattctat aattgttgat aaaggattat tctattgttg
    22861 ctctgataca atagaagtaa aagcatttac acaaaagaac ttctcatctg ctttacttgg
    22921 tggtgaaggc ttcttccaaa tagaattaag tggtactggt gtaatcgttc tggaatgtgt
    22981 tgtacctcaa agtgaaattg tagagtatga attgaaaaat ggtgaagaat taaaggtaga
    23041 tggtaacttt gcaatagcaa gaacatctgg tgttaatttt tctgttacaa aatcagataa
    23101 atctttaatt ggttctgctt taaatggtga aggtttctta aatacattta ctggtaatgg
    23161 tacagtttgg ctagctccaa ctcaagtaat gtatgaaaaa ctaagttttg gagcaatgcc
    23221 tactaatgat aatatgaata ataaagtttc taagcaaaaa tagttgtttt ataaatattt
    23281 attaaatcat tattgatttt ataaagtcac tataaatttt attagattat tattataaaa
    23341 tcagagtgtg gaaatattta tgtgtattct ttaaataaaa attt
//
LOCUS       DAFKMX010000045        23380 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFKMX010000045 DAFKMX010000000
VERSION     DAFKMX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.045"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            350..1582
                     /locus_tag="LZB08_001427"
     CDS             350..1582
                     /locus_tag="LZB08_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBM7314934.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFATSYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1596..2402
                     /locus_tag="LZB08_001428"
     CDS             1596..2402
                     /locus_tag="LZB08_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBM7314935.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2514..3383)
                     /locus_tag="LZB08_001429"
     CDS             complement(2514..3383)
                     /locus_tag="LZB08_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7314936.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3755..5182
                     /locus_tag="LZB08_001430"
     CDS             3755..5182
                     /locus_tag="LZB08_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBM7314937.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5259..5528)
                     /locus_tag="LZB08_001431"
     CDS             complement(5259..5528)
                     /locus_tag="LZB08_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314938.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5531..6973)
                     /gene="nhaC"
                     /locus_tag="LZB08_001432"
     CDS             complement(5531..6973)
                     /gene="nhaC"
                     /locus_tag="LZB08_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBM7314939.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6998..8164)
                     /locus_tag="LZB08_001433"
     CDS             complement(6998..8164)
                     /locus_tag="LZB08_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7314940.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8389..10509)
                     /locus_tag="LZB08_001434"
     CDS             complement(8389..10509)
                     /locus_tag="LZB08_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7314941.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10742..11143
                     /locus_tag="LZB08_001435"
     CDS             10742..11143
                     /locus_tag="LZB08_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314942.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            11186..11479
                     /locus_tag="LZB08_001436"
     CDS             11186..11479
                     /locus_tag="LZB08_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBM7314943.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11494..11934
                     /locus_tag="LZB08_001437"
     CDS             11494..11934
                     /locus_tag="LZB08_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBM7314944.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(12026..12499)
                     /locus_tag="LZB08_001438"
     CDS             complement(12026..12499)
                     /locus_tag="LZB08_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBM7314945.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12499..13287)
                     /locus_tag="LZB08_001439"
     CDS             complement(12499..13287)
                     /locus_tag="LZB08_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBM7314946.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13615..14859
                     /locus_tag="LZB08_001440"
     CDS             13615..14859
                     /locus_tag="LZB08_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBM7314947.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15182..16279
                     /locus_tag="LZB08_001441"
     CDS             15182..16279
                     /locus_tag="LZB08_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBM7314948.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16270..16968
                     /locus_tag="LZB08_001442"
     CDS             16270..16968
                     /locus_tag="LZB08_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7314949.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16971..18035
                     /locus_tag="LZB08_001443"
     CDS             16971..18035
                     /locus_tag="LZB08_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBM7314950.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18218..18922
                     /locus_tag="LZB08_001444"
     CDS             18218..18922
                     /locus_tag="LZB08_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7314951.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18915..20237
                     /locus_tag="LZB08_001445"
     CDS             18915..20237
                     /locus_tag="LZB08_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7314952.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20424..21797
                     /locus_tag="LZB08_001446"
     CDS             20424..21797
                     /locus_tag="LZB08_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBM7314953.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22169..23107)
                     /gene="pdaA"
                     /locus_tag="LZB08_001447"
     CDS             complement(22169..23107)
                     /gene="pdaA"
                     /locus_tag="LZB08_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBM7314954.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8618 a         2902 c         3690 g         8170 t
ORIGIN      
        1 aattatacct gtattctcaa ccaatatagt taaaatacta tgtttaaaaa ttttacataa
       61 acttggttgt tttagaaact atttttttaa tgagtacacg attttaatta aagaatatat
      121 aacaaatttg aaataagtct aatatatgac ttaatagatt aaattaaaat aataaatttg
      181 gactatttat ctatttataa cttaaattat atcaaattga taacaaacta gatattatac
      241 ttttttgtag aatagaatta caattatata aagaaaaatt ctattaatag tgaaaaaaaa
      301 gtagaaaaaa gtagaaatta ttcttttata aagaaaggga gggataatat tgcaagagag
      361 agaagaatta gttttaaatc cagttccaaa agaggaaaga gttggatggt tagctccatt
      421 gtttaatatg cttggttcta acatagctat atctgaacta atggttgggg gaactttaat
      481 tttaggaatg actctttcta atatgattat tacatcaatt atagggaact taatattagt
      541 tgcaattatt atgatacaag gttatatagg ttacaaagaa gggttaaata cgtatgtatt
      601 agctaagggg gcatttggag agataggagg aaagtatctt atatctctat tacttggtat
      661 aactagtttt ggatggtttg gtgtacaagc aggggttgca ggactatcag tacaaaagat
      721 attcccaagt gtaaacctaa cattagttac agttatactt ggattattta tggttgtttt
      781 tgcactatat ggctttaaag ctatggctaa atttaattac ttagttatac caccacttat
      841 gatattaatg gtgtggggag tttttaaagc tttttctact tatggagttg aagctattta
      901 taattatact cctcaaacaa ctatgagtat ggttgaaggt cttaatattg tagtaggtct
      961 tgttatagtt ggtgcaatta tatcacctga ccagttaaga tatacgagac gagtaaaaga
     1021 tatttggatt atcagttttt taggtctagg gattatttca ttattccaac aagttgcagc
     1081 tggtgtaatg tctatgggag cacctacttg ggatataaca gaagtattgg ctaatttagg
     1141 ttttggatgg gtagcgtttg ttattttaat acttgcatct tggtcaacaa acgtatcaaa
     1201 tgcttattct ggtggattag cattaaaaac tatttttcca aatgtaaaaa gaaatgtttt
     1261 gactttagtt gcaggtctaa taggtacaat aatagcagct actggtataa tatttaaatt
     1321 tcagtcattt ttatctttct tgggaatcgc agttcctgca atagcaggaa ttatgtggtg
     1381 tgagtattat tttatacaag gaagaactta taagcataga gaaggaataa attggattgc
     1441 agtaataagc tggttaatag gttttgcaac gtcgtattat agttcaaaaa taaatttctt
     1501 aatacctcct ataaatggaa ttgtagtgag tatggttatt tattatatat taatgaaatg
     1561 ttttggaatc aaagataaat aaagggggca attacatggg tcgtaaggtg aaaataggta
     1621 taattcaaca acacagtgta cttggaaatg ttaaaaaaaa tatagaaaaa gcagtagaga
     1681 tgatagatga cttaggaaaa caaggggcag atataatttg tttaccagaa ctatttgcta
     1741 caggatataa tttagagtct ttaggtggag ttaaaacatt agaattaata agagaacata
     1801 ataagtacat agaggaatca atgtcagaag ctgctaaaag aaataatgta tacctgatat
     1861 ctccatatgg aaccttagaa aaagggtcta cacatgttta taattcagca gttatatttg
     1921 atagaaaagg taagattatg ggagaatact gtaaaaatca tctgtggtca ttagaagcag
     1981 tatactttaa aggaggggaa aaagtagagg tatatgatgc tgattttggt agatttggtg
     2041 taatgatatg ttatgatgca ggttttccag aagtatccag agaattgaca ttaaaaggtt
     2101 ctgaaataat atttatacca tctgcatgga gaatacaaga tgaagatatg tgggatttaa
     2161 atgtatctca aagagcttta gagaacactg tatatacagt aggtgtaaat ttagtaagta
     2221 atgactctaa tttaatacta tttggaaaga gtaaaatatg caatcctaga ggtactgtaa
     2281 taactcaatt agatacatac agggaaaaat atgtattatc agagattgat ttagatgaaa
     2341 taaaaaagta tagggaagat atcccctatt tgaaagacag aaaaagtagc tataacatat
     2401 aattctatac tataatacaa gttcaatata aaatgataaa aagaggatgt atcaatatta
     2461 agattaaatt tattatactt tgatatatcc tctttaatat tatcaacttt gatttaaata
     2521 tttttaaaat agttgataag aaagtttttg aacaacaaca cagctttaga ttcatagttt
     2581 tcgctaaacc atgaaagatt taaatatctt ttaaattcaa tatctttgat atgtaacaac
     2641 ttaatctcag ggtcttgaga taatccccat gattttccac atataaatcc aactccttga
     2701 agtgatttta taagagcata aattgtatat ggtgaatcag actcaaatgc tattttgggt
     2761 ttgaaattag ctgattcgca caggatatca attacctctc tataattctc acccttcgtt
     2821 ataactataa agttttcatc tgaaacttcg cttagatata cttcatcctt taatgataaa
     2881 ggatggttta ttgaaactcc taatagtatt tcttcgcatg ttaaagttat gctattttct
     2941 gagtttagct ttgtaaatga agaagatatg tacaaatcaa aatcactttt tttataagat
     3001 gatggcaaag tatgtgacac attaaaagta atattgggat atagttttct gaaattactc
     3061 aaaagtgatg gtaacacatt agccgctgca ggagataata tctttacttc tcctgaattt
     3121 tctaaagaag tgtcttgtaa ctcctttttt gaactttcta ctaaatctaa aatgctatct
     3181 actcttttta gaaacaatct tccatactca tttaaaacaa tatatcttcc ttttctgtca
     3241 aataactgaa ctccaagttc tttttctaag gaagatatag ttttgcttaa agctggctgt
     3301 gctatattta acttgtgtga tgcttttgtt atgtgctcca ttctagcaac agtttgaaaa
     3361 tacttaagat gcaataaatc catagtataa aatacacctc ttttaatatg acttgagtta
     3421 tatcaatttt ataatcaact ccacattata cattataata taaagtttat ataataaact
     3481 acaatatatt aaaaagttat ttaaattgtt atttatattt tgtacataat ctaattaaaa
     3541 aaagtaactt taaatgatta actaaggaaa tattgttaca aactttagtt atataaaaaa
     3601 ttcatttgat gagcaaagta ttaatgtcaa gtaagaataa aagcttatta gataagaaaa
     3661 ataatatttt ataataatga tgttacttaa attattatgt tatactattt taaataggaa
     3721 atatttagat actataattt tagggggagt caagatgaaa ttaactaaat caatatcatc
     3781 aatgctgtgc ttatctctag ttttaagctt atcaccagta gcatatgcaa atgaaataag
     3841 tagaacaaca gttgagaata ctttagtagg tcaggatagg tatcaaactg ctgttgaagt
     3901 aagtaaaaaa ggatggagta gtgcaaatga ggctgtagtt gtaaatagtg aatctattgt
     3961 agatgcatta gctgtaacac catttgctaa attaaaaaat gctcctatat tattgacaga
     4021 aaaaaataat ctaaattcac aaactgagaa agaattaaaa agattaggag ttaaaaaagt
     4081 ttatataatt ggtctatcta gttctgtatc aaatgatgtg caaaaacagt tagaatctaa
     4141 taatttaact actgatagag ttggtggtag tgatagatac caaacagctt tgagtatagc
     4201 taaaaaaata gaaggattaa aagatatatc agaaatagct gtagtaaatg ggtacacagg
     4261 tcttgcagat gcagttagta tagcttctgt agctgctact aatggaatgg ttatacttcc
     4321 agtatctgat gcatctggta tatctagctt taaagatttt atagaatcta agaatataaa
     4381 taaatcatac ataataggct ctactaatgc tgtatcagat aaaataaaac aaagtttacc
     4441 taattcagaa agaataggtg gagcagatag aaatgaaact aatggtaaag taatagaaaa
     4501 gttttataca tctaataaat taaacaatgt atttgtggct aaggatggaa tgaaaaatca
     4561 aaatcaactt atagacgctc tagcagtagg agtactagca tcaaaagaaa attctcctgt
     4621 actaatcgta ggagaacaat taagtgcaac acaaaagagt gtattttcga ataaaaatgc
     4681 tacctctata actcaagttg gtagtggagg aaatgaaaat gcatttaaag aattaaaaga
     4741 tttacaggca aatagtaaca ataagagagt aatgtatgta actaatacag ataaggtaaa
     4801 tataagaaat gatgctacaa tagaagcttc tgttatagga tatctaaata atggtgatga
     4861 agtagaagtt ttagatgtgt taaaaactgg atgggtaaaa ataaaataca atgaaggtat
     4921 tggatatgtt agtgggtctt atttgacaaa taataagcct gataatagta atgaaaatat
     4981 aaaaataaag tatgtaaaag aaaaagatgg tctaaatgtt aggaaaggac caagtacaga
     5041 agatgaaaaa ataggtcaac tttcatatgg aagcaaagta gaaacagtag aaatgtttgc
     5101 tactggatgg gtaaaaataa agtataatgg tggatatggt tatgtaagta atgattactt
     5161 atctgatacg ccagttattt aattataagt aacaataaaa aaactgcttt aatttgtaaa
     5221 gcagtttttt tataaaaatt taagtctaat aactatttct attttgatac cttaatcctg
     5281 ggatttatta ttatggatat tatcaacata caaacactcc aaatccagtg tctaattcca
     5341 aacttagata ttaataaatt cagtcctgct atggtaaata agcagtattt aattacaatt
     5401 aaaagtgtat ttatagaaag ccccttcata gaaagtatcc taaatccaat aatagacatt
     5461 ctaagtccta taaatgccaa taatatacct gcaatatcct gaattaagta atagtattca
     5521 aatttcatat tcaatcaact cttctctgag cagtatcaaa tatttctaac tcttcaattt
     5581 catcatctgt gtatgtaagc atagtaaaac cagttacacc ataaataaat gaaattatag
     5641 gtgtaatcca acttaagaaa caatatggaa taaattctag tgggcttaca ccaagagtct
     5701 gagcacagaa aacagcattt gaattccatg gtattaatgg accaccaagt gtagctgtat
     5761 cttctataat tctagataga ttttgtggtt ttaatttaaa ttctttgtat attggagaca
     5821 ttagagttcc gacaaataca gaagtgaaca tcattgatga actaaatgca tttgcaaagt
     5881 agcttactat aaaagtagac ccaaccaatt tagttcttga ttttcctatt ttagttataa
     5941 gaggttgaag cagtacttct aagaatccag ttttttggag aataccagca ataacgaaaa
     6001 ctacaaatac tagtaaaacg gtttcagcca tactcattat tccaccacga tttaatagtt
     6061 tgtcggcata gacaaatcca gtatctatag taaatcctga tagcatgtaa tttaaaacat
     6121 cacctatttt ttctccttct tgaaatactg ctataataag tcccatgatg gctgaactta
     6181 gtatagatat aatgggtggc ttttgtagca ataaaagtaa taataaaaat attattggaa
     6241 tcattgctac taaaccaata tgaaagtttg aatttaatac atccttgatt tgatttattt
     6301 gaacatagtc tatagtatta tttgagtact taaatccaat tactgtatac agaactatac
     6361 atattacgta tgcaggtcct gtggtataaa acatatgctt catatgtgta attacatctg
     6421 tcttacaaac tgctgcagct aagtttgtag aatctgatag aggtgacatc ttatctccaa
     6481 agaaagcacc agaaatgact gcaccagctg ttaaaccaag aggaataccc atactagtac
     6541 caatactcat cattgctata ccagaagttc caagagtccc ccaagatgta ccagtagcaa
     6601 cagaagttaa tgaacatacg attaatgttg taagcagaaa atatttaggg gttattattg
     6661 ttagtccact atatactact gttggtattg taccagcagc tatccaagct gctattagta
     6721 caccaacaga aagtataatt aagtttgatt gaaaggcatc tttaccaact tcaaatccaa
     6781 aagcttctat ttcatcatta gtataaccaa gtttcatggc tgcaggaact actataagcc
     6841 atgaaagtaa aaacattgta gttatagaag cattaaatac aactatacca actgatgtta
     6901 gggctattat tgcaagcatt actaacaagg aatatctaaa tgaaggtatt tttctttctg
     6961 tattcatttt catagtgcaa attctcctta aatattttta gttaattgca ttttttattc
     7021 ttataagagc ttcttcaagc atacttcttg gacaagctaa gtttattctt tgatacccac
     7081 ttcctccaat gccaaagctg tttccttgat ttagagcaac tttacctttt tgtacaagta
     7141 tactttctag ttcctcatca cttaatccaa gtgcactaaa gtctacccat aataaataag
     7201 taccttctgg ttttctaact ttaagttttg gcatattttc atttatatat tttatagcaa
     7261 aatctatatt agattctaaa tattctaaaa atgattctag ccaagattca ccattattat
     7321 atgaagcttc agttgcaact aagctaaagc aattatttct ctttatatca attctggtaa
     7381 aggcatcatc taacaattta tagtcttttt catcaggtag tattacatat gatgattgaa
     7441 gaccagctat attaaaagtt ttagttggag ccatacaagt aattgtattt ttttcaaatt
     7501 ctttagaaat agatgccata ggaatatgct tatgtttttt taatataatg tcactatgta
     7561 tttcatcaga tatgattttt acattatgct taagacaaat atctcctagt ttttttaatt
     7621 catcttttgt ccatactcta ccaactggat tatgaggatt acaaagtatg aataatttta
     7681 catctttaat cttgttttca atgtcttcat aatccattat gtagtttcca ttttctagtt
     7741 tttgtaatgg gtttataatt agttctctat tgttattttt tacaacacta ttaaaagggc
     7801 tgtaaactgg ttcttgtatc attattttat catttgcttt tgtgagttca tttattaaaa
     7861 gacttatagc tgggataaca cctggactat aaattaacca ctcacttttt attttccagt
     7921 tatgtctcct atatagccag tttactatag attcattgta agaatctggt cttgttgtgt
     7981 agccataaat ctcttgttct agtctatttt ttaaggaatc aattatacaa ggtgcagcct
     8041 taaaatccat gtcagccacc cacataggaa gtaaatcatt agttccatac tttttttcca
     8101 tttctgacca tttagatgaa aaattattgc ttctatcaac aatctcatta aaattataat
     8161 tcattgtaaa tacctccagt aattttaatt ttatgttaat aatattaagc aaacaacgtg
     8221 ccaatattga taataaaaaa tatttattca caagttaata tttaatatta agtaatttca
     8281 gagcatagat aagataaaaa tattaattat acttaaaact ttgtggaaaa ggttatttct
     8341 aataaatttt taaatatatg aaaatgtaat taaataggtt aaataggttt aactgtttaa
     8401 cttgttagac ctatttatat tttttagaaa ttctactcct tttgatgtta taattgaacc
     8461 tccacgacca acaagtatgt ctacaagccc aaagtcttgt agttctaaaa gcatatttct
     8521 aatctgttgt tctgtcaaaa atctattttc ctctaaagct attttacata agctttttct
     8581 acctattctt tcttttaaat cataagcctt ttttaaatta tccaatataa atatgtattc
     8641 atctagtgac cttttaaaat tatagtcgta tttcatgatg tttttgtctt tgccaatgtt
     8701 gaattgatac ttttttatat tatcacttac tttattacaa acggttctac tataatttga
     8761 atctatagta tccaatatat attcaggtaa atcacatatt tctataatat ctttttctaa
     8821 gtaacagaag tattctccta agtttcttaa ttctcttaca tttccttccc atctatacat
     8881 tttaaagatt tcttttactt cctcacttag aataaaatta catggaatat catattttaa
     8941 tgaaccaaaa atttcaaata tatcagcagg tctttcccta agtggatgta ttttaagggg
     9001 aagaacattc aacctaaaat ataaatctct tctaaacata tttttagata caagttcttt
     9061 taaatttcta ttagtagctg caattattct tacatcaatg tcaatgacat tattagaacc
     9121 aatacgtcgt acttgttttt cttgtatgac tctaagtaat ttagattgag aatttaaatc
     9181 catttctcca atttcgtcta aaaatatagt tccattgttg gctaattcaa ataaaccaat
     9241 tttcccacct tttttagcac cagtaaaagc cccttcatca tagccaaata attcactttg
     9301 cagtagattt tcttgaaaag tactacaatt tactgcgaca aaaggaccat cttttctcct
     9361 agaagcacta tgaatggatt gagcaaataa ttctttacca gtaccacttt caccaattat
     9421 aaggatagat gaatttgatt gagccatttt attagcaatt ttttttgtat taatagtttg
     9481 tatactagaa cctaatatat catcaaatgt gtatttactg atattaccag agtttactag
     9541 ttgtgctctt aatttagctt gctttttttc taattgagaa aactttgtca tctttagtat
     9601 aaatccatca aatacattta attgtatatg tttaatctct aaatttatat caatatgatt
     9661 tattttaatc aatttctcaa aaaatggagt tttattggtc aatatgcttt gaaaattgat
     9721 gtcttttatg caatcaccaa caaatgagtc aatcatttca tttgcattta tagacaaaat
     9781 ttttctagcc atatgattat agaattgaat tattccatcg ttatttgtgc atataattcc
     9841 atcatcaatt atagatagta acaaatcaaa ttgtctttca aacttatttg catctaacaa
     9901 taactcacct gtactataac ttgtaggaat tattttttct acgtattttt ttattaggtc
     9961 atcttttatt agatgtttta atttggtttt tgtagcaatt tcaacaatac aacttaaatc
    10021 tattattctg tattttaaat ctataacttc acaattttta aacttaggca agattttttc
    10081 gccaggagtt aggattacag catctgtggg agtaaactca atctctggat agcagggcag
    10141 gatatttata ttgtctattc ctaatctatg tattaaagaa attgtttcta ttgccatatc
    10201 tttacttaag ttatatacat aaataatttt atctttttct aatttggata tcatatcaat
    10261 actgttcttt tcaaatgtca acttgggaac aatgatttga gcatcatagt gagataattt
    10321 tactatttca tcatatttta aataagcaga taggagtata agtttttctt taaaaaactt
    10381 taaatttccc tcttcaaatg agtataaatt tatctttaga ttatcaccta agaattgcct
    10441 tatttgattt tcataaattt ctccagcgtc tttttttaaa cttacaatag ctaattccat
    10501 ttgtttcatt ttaacctctc atacaaattt gacttatttt agattacata ataattaaat
    10561 tagattaaat aatattattt aattattatt ataaatttat tatagactac acattgaagt
    10621 aaaagaagta gctaaaatta ttccttttat ttttgttttt tgtatctatt tggtataata
    10681 gtattagagc gtataataat ttaggcaaaa ttttttaagg aagactagag gagaatataa
    10741 aatgaaaaaa tgtctttact gtaataggga tataaagtca ccaaataata aattagctat
    10801 aaaatatgat gacaatatga atatatatcc atgtcgtgca gaatgtaagg aaaaaattga
    10861 agaatatata gcaaaaaaac ctacatataa gtttataaat atattagagg ttcttttagg
    10921 tgtgggcgga atattctgct ttttgagtaa atggactatt gcagcccaag ttgtacttgg
    10981 aattgatgca ctggttatat ttttattccc attggcagga tttaagatga atggaaagtt
    11041 aagtatggaa caaactaaga atcaaagtag gtctattgca atatcaattt tggcatttat
    11101 agtaataata actgtattat attttaaaca aattggtaag tagagcataa ttgtataaga
    11161 catcattaga gtatgaggtg agtttatgga agaaagaaaa ttagcaccaa acaatgagag
    11221 ggaagctctc ataaaaagat taaaaagaat agaaggtcaa gttaagggta tacaaaagat
    11281 ggtggaacaa gaacgttatt gtgttgatat attggttcaa atatctgcta taaggtctgc
    11341 tataaataga gtaggcacaa ttatcttaga gaatcatata aaaggatgtg tagcagaaag
    11401 cattaaacat gatgatgctg ataaaactga agagatgata aaagagctta tggatactat
    11461 aaataaattt acaaaataag gaggcggtta gttatgaatc aacaaggatt tattatggaa
    11521 attgttgatg atagaactgc aaagttaaaa atgcaaagac attcagcttg tgctgcttgt
    11581 ggaaaatgtg ctactacaaa ttcagcagag tcaaaagaaa tattagtaga agtagacaac
    11641 acaattggtg caaaagttgg tgaccatgta gaagtgagta tggataatat gaatgtatta
    11701 aaagcagctg taatggctta tataatacct cttatagcac ttcttgtagg aacaataggg
    11761 acttattata tattgggggc aattggaatg acaactggag ttgaagccat aagtggatta
    11821 gttggaattt gtcttacaat aatctgttat ttatacttaa agaagaatga caaaaaattt
    11881 agagacagta gagaatttat accagtaata acaagaatat taattgacct ttaagataat
    11941 tataattaaa aaatagcaat gtgcttttta aataaatact caatacgtat ttgtagagtt
    12001 taagtacatt gctattttta tatttttatt ttgattttct gtaaggaata aagttttttc
    12061 tgattttcaa aagattcaat gttcttacaa aagaagttac aactaataca ccaagtgcta
    12121 ttgcagcact tagaaatagc gcatctgtac cttttgacat agccaaagaa taatttttct
    12181 gaatcaaaga caacattgta ttatataacc caattcctgg tattagagga agaatacctg
    12241 gaattacgaa cacaatagct ggttgcttta gttttcttgc taaagtctca ctgcacgctg
    12301 agaccaaggc agcagcgata aaacctgcaa aaactgcatt tgtagtaaaa ttaaataaat
    12361 aaacatatac aatccaacca attccgccag ttactccagc tggaagaagt agatatagtg
    12421 gagcattaaa gaaaacagca aatccagcag ttgcaacacc tgaaaataaa aaatgcaaaa
    12481 tcatagacat atcagtcatt agaaaggacc tcctattcta taccatgtat caaggacaaa
    12541 accaactcca gaagcaattg caataataat catagcagca tcaaaagcac gagaaattcc
    12601 tgatattaaa ttccctgata ttaaatctct aatagctttt ataaaagcaa ctcctgggag
    12661 tagtggcatt atagcgccga ctataagcat tttagggtca tctaaaatgc ctatttcaac
    12721 tagtagaact cctgaaagtg caattaaaaa agaagatact aaacaggaaa atgcagatat
    12781 tgcacttatc ttcatcgttt tatcataagt aattgcagca aagattgcta ttataaacgt
    12841 aagtataaaa tttaatacat aattccctcc aagcaaacaa gcaaaacaag cagatgcaat
    12901 tcctgtacaa gagtaaaata accataaagg ataaggtttt acatcttgga tttcataaag
    12961 acgtgcaatg gcattatcaa tatcatactc tttgtcacta acaaattctc gagaaaaact
    13021 gtttaataaa gaaatcttat ttaagtttat acctcttgat tgaatcgttt tcataaatga
    13081 aaagccatca aatttctcat ctgaaataat tattactgta ggagttgtaa aaatatttac
    13141 atgataaaac cctcttgacc tgcatattct aaggacactg tcttcaaccc tagatgtttc
    13201 agctccattt tttatcatca gttcccctac aaatagagct agttttagaa tatttttctt
    13261 atattcaagt tcctcatttt gattcataaa tacacctcca aattaaaaaa cgtaatattg
    13321 aaattatatc aaatttttaa gtaatattcc ttaatatcta tataaatata ttatcttttt
    13381 tttgtttcaa aatagtcaca tttaaattat atccaattaa aagaattaga caatattatt
    13441 tattatattt taacttaaaa ataaagaaag taactatgtt aaatatggaa aaatggcaaa
    13501 acacttgact attaattggt aaaggactat aataataaat tatcatattg ataaaaacga
    13561 aaattatgaa atgtctgtaa aaataaaatt taaataataa ctaggaggag taatatgttt
    13621 gagaacttga aaaaagcgga ccctgagatt tatgaaagta tgaaaagaga gttaaaaaga
    13681 caacaaagaa atattgaatt aatagcatct gaaaacatag tatcagttcc agtaatggaa
    13741 actatgggaa gtcatcttac aaataagtat gcagaaggtt actcaggcaa aagatattat
    13801 ggtggatgtg aatacataga tgaagttgaa actcttgcaa tagatagaat caaaaaaata
    13861 tttggagcag aacatgctaa tgttcaacct cactctggag caaatgcaaa tattggtgta
    13921 tattttgcta tgttaaaacc aggtgatgtt gttatgggaa tgaatttatc tcaaggtgga
    13981 catttaactc atggtgctcc agttaatatt tctggtcaat attataaatt ttatgaatat
    14041 ggtatagata aagacagtgg acttatagat tttgatgaag ttagaaagat agcacatgaa
    14101 gtaaaaccta aaatgatagt tgcaggagca agtgcatatc ctagagaaat agattttaaa
    14161 aagtttagag agatagctga tgaggttgga gcattgctta tggtagatat ggctcatata
    14221 gcaggtcttg tggctgctgg tcttcaccaa aatccatgtg aagtggctga ttttgttaca
    14281 acaacaactc acaaaacttt aagaggacct cgtgggggtg ttattctttg taaagaaaaa
    14341 tatgctaaag acatagacaa agcaatattc cctggtattc aaggtggacc tcttgaacat
    14401 ataattgctt ctaaggcagt ttgctttaaa gaagctttaa gtgatgagtt taaagaatat
    14461 caggtacaag ttgctaaaaa tgcaaaagcc ttagctgaag aacttattaa aagagatttt
    14521 aaattgattt ctggaggtac agataatcat ttaatactat tagatttgac taataaaaat
    14581 ataactggta aagcagcaga aaagagattg gatgatgctt atataacagc taataaaaat
    14641 acaattccat ttgacccaaa tggtgctctt gttacaagtg gaataagact tggtactcca
    14701 gctgtaacta ctagaggtat gaaagaagaa gatatggcaa taatcgctga ggctatagac
    14761 ctatgtttaa cttatgatga agaatctaag gctagaacat tggttgttgg tttaactgaa
    14821 aaatatcctt tatatgaaga atataatatt atggattaat tataaagaac aactaaataa
    14881 aatacattta aataattaca atgattgtgt atgtatttga atatatttag atataaaaaa
    14941 ggtgtctcaa aatgaacttt ttagtttatg aggcactttt ttgtatggaa tcgaacatat
    15001 ttgtattatt taaataataa aaaatatggt tatctctatt tttagatggt ctctttgtat
    15061 tttttaatta aaactttaag atttcttaag aattatacta ggtttttcta aagatttaaa
    15121 gtctatactt ataagtatag taatcatatg aaaagaaaag ctttaacgag gagggaatct
    15181 tatgaaggta ttaattctta caggaaaatt tggtatgggt cattactcag catcaaattc
    15241 tttaagtgaa gatataaaag caaaatttga taattcagaa ataataatta aagatatatt
    15301 tgagtacatt atgcctaact attcagataa aatgtacaag actttttcta ttcttgtaaa
    15361 tcggggaagt agtctttata atttgtttta taaatgtgct gaaaatggaa agaaagatat
    15421 aaaatttact ttttcagatt attttttaaa taaattagat acattacttc atgaagtaca
    15481 accaacagta gttatttcaa cttttccatt ttgttctcaa ttagtatcaa gatataagga
    15541 aaaatataac tcaaatcttc ctttaataac atgcataact gatataagta gtcactcaga
    15601 atggattagt aaaaatacag actgttattt agtagctagt aaatctacta aggaagaact
    15661 tgtttttaaa ggaatagatg aatctaagat taaagttaat ggtattccag taaaaaaaga
    15721 atttaaaaga atagaacatg taaatcattc aactaaaaaa aatattctta taatgggtgg
    15781 aggattagga ttactgccta aatcagaaca attttacaaa gaattaaata gtttagaagg
    15841 agtaaaaact actgtaataa ctggtaataa taaaaaaatg tactataaac tatatggtag
    15901 atatgaaaat atagaggtag taggttacac caatgaagta tataaatata tgaaagattc
    15961 tgaccttatt atttctaaac caggtggaat aacactattt gaaacaattt attcagaact
    16021 tccaatattg gcatttaatc catttttaca acaagagata gataatgcta gttttatatt
    16081 aaataatgaa attgggagaa tacttggtaa aaataaaaag tactatgtag atgagataaa
    16141 agatttaatt tatgatgatg ctactcttaa agaaatgagc agtaacatga aagaattaaa
    16201 aaaacaattt gataataata ccttagagaa tattttattt tcacttgatg aacaaggagc
    16261 gtgtagagaa tgtatgtagt aggtttaatt atcgttgttg cattgatttt tttagtccat
    16321 tcaattatac ctacatacta taataaacta ttaaataaaa aggtattaaa aaatatggct
    16381 ggagaaaatg aaattgcact tacatttgat gatggtccag ataagagata tacagaaaaa
    16441 ttactagatg tgttaaaaga aaatgatata caagctatgt tttttgtagt agcaaagaat
    16501 gctgaaaaag agcctgaaat tataaaaaga atgttgagag aaaatcatat agttggttta
    16561 cattcattgg aacacagaaa tgcatggctt tatagttata gttatgttaa aaaagatttt
    16621 attgaaagta caaatataat gaaaaattta ggtgtagatg taaactacta tcgtccacca
    16681 tggggtcata ctaatatatt ttcaaattca tttgttaaaa aatataatct aaaaatgact
    16741 ttgtgggatg taatggcaga agattgggag aaagatagta cagtagacat tattataaac
    16801 aagttaatga gtagaacaaa agaaaattct ataatttgtt tacatgatgc aggtgaaaat
    16861 tcaggtggtg cagtaggtgc acctgagcgt actattgaag ctttaaagat agctattcca
    16921 aaacttaaag ccagtggttt aaagttcgta acaccagaaa ggatgtagtt atgataagta
    16981 aaactgaaaa gaaaaataaa tgcctaggta gtgtagcttt tttagtttta ctaatgggca
    17041 taacaggata ttttgttttt agagggcagt cagttgaatc attgataaaa tcattaaaag
    17101 gtgcaagccc tatgtttata ctaattggat ttgcaatgat gtttatatat gttgcctgtg
    17161 aaggtataaa tatatactta ggtatgaaag ctttaaacca aaagactact cttttaaaat
    17221 gtatgggata tgcttttatt ggattttatt ttagttctat tacaccttca gcatcaggtg
    17281 gtcaaccagc acaagtttat tatatgaaaa aagacgatat aaatatatca tattcttcac
    17341 ttatactatt agtcattgtt gttattcacc aagtagttat actggcatat agtggaataa
    17401 tgttcattat ggaacgtgag tttatattaa ataatgtaag tggtatgaac attttactaa
    17461 tatatggtgt aataacaaat gttgctcttg tcattggagt aattgcaatt atattttcaa
    17521 aaaaacttgt aaacaatttt ataatatcaa taacaaattt attaggtaag ttaagaataa
    17581 taaaagatgt tgaaagttca agaaaagtta taatatctca aattgaagag tatgttaaag
    17641 gtgctcaata tataaagcag aatccaaaac ttgtagtcca aattttagtc ataactattg
    17701 tacaaataac agctatgttt ttagttcctt tctttgtata caaagctttt catctatcaa
    17761 catatactgt atttgagata ttagcgatac aatccttgct taatattgct gtttcttcat
    17821 taccattgcc aggggctgtt ggagcttctg aaaatagttt tatgacatta tttaagatat
    17881 ttttccctgg tcacttatta gttccggcaa tgttacttag tagggggata agtttttatg
    17941 catttgtagc tataagtgga ttgatatgta tagtagtaca tgtaaaatct tctgaaaaag
    18001 taaaagcagt gaaaaaagtt gcttatgtaa gataatatta tatagattat ttttattaat
    18061 atgtcaattt gtgtgtatat tttaattaag ctttaaacat gtttacagga gtagaaacta
    18121 ttaaaacgtg tgacattgct aagcttttgc taataaagta taatatttta tatagtttga
    18181 aatatttaag aaatatagca aaaaaaggag cttggtgatg aataaagaga ttccaaaaaa
    18241 aattttagta gctgatgata atcctgaaat tagggaaatc gtagaaatac ttttaactgg
    18301 tgaaggttat gaagttatta tggcaacgaa tggagaagaa gctgtaaact tagtagatag
    18361 tacgattgat ttgattattt tggatgttgt aatgcctgta aaaacaggat ttgttgcatg
    18421 taatgaaatt agagaaaaaa cgacagcacc aatattattt ttaactgcaa aaactcaaga
    18481 ctctgataaa gttattggat tttcagcagg aggagatgac tatttatcaa agcctttttc
    18541 atattctgaa ctaatttcaa gggttaagtc tctacttaga cgttattata tataccaggg
    18601 aaaggaaaag gaaagagagc caaatatatt aaaaacaaaa gagttatgtg tcaatttaga
    18661 tactcaagat gttacactaa atggagaacc tgtgtttttg acaactatag aatatagtat
    18721 attaattctt atgttgaaaa atagaaaaaa agttttttcg tctgaaaatt tatatgaaac
    18781 tatttgggat gagcagtatt tttatacagc aaataataca ataatggttc atattagaaa
    18841 tttaagaaaa aagttagaaa aagaccaaaa aaatccacaa tatataaaaa ccgcatgggg
    18901 aaaggggtat tatattgatt aacaaaatta agccatctaa gttgggagtt atactattga
    18961 tatatgtagt aatctcagct gcagtggctt tcattttaaa tataccattt tactctgctg
    19021 ttgaatctat agtaaaagat gtatcagaag ggaaagtata ttttaaaaga gaagtaaatc
    19081 aacttatgga taattttcaa acttatgtga ataacaatgg tataagagca gatgatggag
    19141 atgatatagc aaaatggaat agtgataatt ggtttgtttc tatgaagata tataaaggaa
    19201 ataaggtttt ttatgataca ttatactatc ctaatgaagt acctgataag agtgaggaaa
    19261 atgagtttta tagcagtccc atggctagaa actatgagat acaattttct gatattaaag
    19321 ctaatatagt tgtagtagct ttatttagat ttagatttca aaattttata gaagcaattt
    19381 ctattacatt tatggcactt acatttatta taacattttt actgttgttt agtagaaaga
    19441 taaagtacct aaggaaaata gaaatgggta taaagatagt agagagtggc agtttaaagt
    19501 atagaatacc agtaaaaggt aatgatgaat tatcatcact tgcatctagt attaatgaga
    19561 tgagtcatac attggagaat caacttataa aagaaaggga gatgaagaat aaaaataaag
    19621 atatagtaac ttcaatatca catgatgtta gaactccatt aacgtcagta atttgttatt
    19681 tagatttgat taaagaccat aaatacaata gtcctgagca attggaacaa tacataaaaa
    19741 atgtaaggtc taaagcatat caaataaaag atttaacaga tgaccttttt acgcattttg
    19801 ttaattctgg agaggaagtt tcaaaagggt atgaaaaaat aaatgctaat gaatttattt
    19861 ttcaattact tttagatagt tcatatacat tggaggagaa aggatttcat ataacaatag
    19921 aaaatgaact ttataaccaa tatcatattt atgtagatgt tacacaattt agaaggatat
    19981 ttgataattt atgttctaat ataataaaat atgctaggaa agataagcct atagtatttt
    20041 taattaaagt agaggattct aaattactaa ttatacaaaa aaatagagta agtaattttt
    20101 ctaagaatgt agaaagtttt ggaattggta taaaaaattg tgagaaaata gttgcatatc
    20161 ataatggaag cataaatgta aattcagatg ataaagaatt tgctattgaa atatctattc
    20221 cagcaaaaga atcataaaaa tctaaattat atattgtgta attgctatta tatattgtgt
    20281 aattgcttat gtaaaattct atgaagcagg aagcatgtga ctttagttat gggatgttca
    20341 atattgtgta cctctaggca agtaacaagt ttacttataa gaccatataa tattattatc
    20401 ttaaaaaaag gggacataat aatatggttt tgacaggagc acattatatt tacattctat
    20461 ttatggtaat tatattaatt actatgatta tgaaaaagga tacaatagta ccatgtatat
    20521 taggtgtttt ttttatgggg ttgttctttg aaaaaaacat ttttggtgct ataacagcag
    20581 tgtttaactc ttttattata tctttaaatg agttgggtcc aattatcttg attatagcta
    20641 ttatggttgc attatctaaa gccctagaag ccaataatgc tatacagtat atggtgagac
    20701 cattttcgag agttataaaa aattctaata cagcattttt tgtaactgga tttgttatgt
    20761 tagttctttc ttggtttttc tggccaactc cagctgttgc cttagttgga gctgtatttt
    20821 tacctgttgc tatgagagct gggctaccag caattggtgt tgcagtagca ttaaatttat
    20881 ttggtcatgg tcttgcctta tctacagact ttgttattca aggagctcca agtataacag
    20941 ctggtgcagc aggtgttgca gtatcatatg ttataaatga tggtatgatt ttgttttggg
    21001 tgatgggaat cgtaaccata tctatggcat tttatacatt aaaaagagat attaacaaag
    21061 gaatgtttag agaagaacta aaaggttttg aaagtgaaga agtagaagag ttcaatggaa
    21121 aatctaaaat agcaactatt ttagtagctt tgggatttct tgctgatata attgctatgt
    21181 atgtatttga cctaaaagga ggagatgcat cagctctttt gggtggaaca gcagtatttt
    21241 taataataat tataaatgtt ataaattttg gcaaagatag tcttgaaaat gtatgtgaga
    21301 atataattga tggttttgtg tttgggataa aaatatttgg agcaattata ccaatagcag
    21361 catttttcta tatgggtgaa gttgcaccat tgacaggtgt atttggaaaa gtattagcac
    21421 caggttcaca aggtttatta tcagatatag gcatagctct ttctcaaaca ataccactta
    21481 ataaatttgc cgtatcaggt atagaaactg tagtgggagc aataactgga cttgatggtt
    21541 ctggattttc tgggatttca ttagttggtt cacttgcctc tgtatttgga actgctataa
    21601 atgcaagtgt tggtgcacta gctgcattag gacaaatcag tggtatatgg gtaggtggag
    21661 gctgtctagt tccttggggt cttatttcag cagcggcaat ttgtggagtt agtccaatag
    21721 aattggcaaa gcgaaacttt gtcccagtta taaccggatt ggttgtaaca accatagtag
    21781 caatgtttat tatttaataa atcgaataaa tctttaattg tataaattaa aaatagatgt
    21841 gtagctataa tttattataa ttgttttact aattctaagc catacatcta tttttattga
    21901 tatttttatt agtattcttt aataaaataa tatttttcat tatactatac ataacatcta
    21961 aatataacat ctaaaatact gttatctata tttaatatct tgccaaattt tttgatgttt
    22021 tattgccctg tcccaccaac atatataagc tatcacaagt ttttacctat actatcacca
    22081 aacaaaaatt ctaattttaa tttattattt tataacctat attatattaa ctatattagt
    22141 ttgtagactt tagatgtaat tataaatatt aatactccaa gagttcaaat tcatatccta
    22201 gttttcttgc atttgatata aaatcattta aaatttctgt actagttttg gaaactgcat
    22261 gtagtaacaa gatagaacca tcatgtagat tatccattat tttttgcgtt gcttcttcat
    22321 gtgatggttg tttatcagta tcccagtctc tgtatgcaaa actccaaaat acagtcttgt
    22381 atcctaaatt tttagtcatt gccaagcttt tttctgagta ttttcccata ggaggtctaa
    22441 aaaatttaac catatctttc ccagttacat ctttatataa cttttcaaca tcatataatt
    22501 cgtcattaaa gttttttaga ttgcttgttt ttgttggcat agatggatgg gttttactat
    22561 gatttccaac tatatgtccc tcagaaacca ttctttttac taaatcttta ttttctttta
    22621 tatatggagc tgtaacaaaa aaaactgctt taacctggtt ttgttttaga gtgtcgagta
    22681 tctttgtagt gtatccattt tcataacctt catcaaatgt aagatatagt gttttttgac
    22741 catcttttgt aggtccattg tatagggcat catagtcaga aaatttaaat tcaatgtccg
    22801 tatttacttc tggtgtttta tgcttattgt ttggtatata aaaccaatca agagtagtgt
    22861 tatctaaaga agatacatct aatttattat tatcattatt agtagtggct tttgatggtt
    22921 tatctgttcc gtctgaaact attacattgg aagtatcttt accactatct attttttcct
    22981 tatcttcttg taactgtgtt tccttatttt gattttcgtt ttgattgttt tgtgaatttg
    23041 aacatccaac aataaaaaaa atagaaatag aaagtagtgt taaataatgc aatacttttc
    23101 taaacatttt gaagctcctt tctaaattaa taataattgc atataataat aattatatga
    23161 ttaattacaa ttgcatatag tattattata attggaaatt actattaata atagttatat
    23221 ctttaaaaat aaagtcacaa tagtatgaca aagggtgaat aatcaataaa tttaaaaaaa
    23281 aatgaaaaaa atagttgacc atattatgag aagatgttat tataataaat gtgctcaaca
    23341 agaagtaaca aaataacaag cacaaagaag ttaattgaat
//
LOCUS       DAFKMX010000046        23288 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFKMX010000046 DAFKMX010000000
VERSION     DAFKMX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.046"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            235..1839
                     /gene="rho"
                     /locus_tag="LZB08_001448"
     CDS             235..1839
                     /gene="rho"
                     /locus_tag="LZB08_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBM7314955.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKNNKEFVKSIKNL"
     gene            1992..2192
                     /gene="rpmE"
                     /locus_tag="LZB08_001449"
     CDS             1992..2192
                     /gene="rpmE"
                     /locus_tag="LZB08_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBM7314956.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2337..3230
                     /locus_tag="LZB08_001450"
     CDS             2337..3230
                     /locus_tag="LZB08_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBM7314957.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3248..4096
                     /gene="prmC"
                     /locus_tag="LZB08_001451"
     CDS             3248..4096
                     /gene="prmC"
                     /locus_tag="LZB08_001451"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBM7314958.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            4140..5204
                     /gene="prfA"
                     /locus_tag="LZB08_001452"
     CDS             4140..5204
                     /gene="prfA"
                     /locus_tag="LZB08_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBM7314959.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5333..6013
                     /locus_tag="LZB08_001453"
     CDS             5333..6013
                     /locus_tag="LZB08_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBM7314960.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            6042..7082
                     /locus_tag="LZB08_001454"
     CDS             6042..7082
                     /locus_tag="LZB08_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBM7314961.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            7099..7548
                     /locus_tag="LZB08_001455"
     CDS             7099..7548
                     /locus_tag="LZB08_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBM7314962.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILIQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7565..8017
                     /gene="rpiB"
                     /locus_tag="LZB08_001456"
     CDS             7565..8017
                     /gene="rpiB"
                     /locus_tag="LZB08_001456"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBM7314963.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            8053..8682
                     /gene="upp"
                     /locus_tag="LZB08_001457"
     CDS             8053..8682
                     /gene="upp"
                     /locus_tag="LZB08_001457"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBM7314964.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8983..9798
                     /locus_tag="LZB08_001458"
     CDS             8983..9798
                     /locus_tag="LZB08_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBM7314965.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            10024..10461
                     /locus_tag="LZB08_001459"
     CDS             10024..10461
                     /locus_tag="LZB08_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBM7314966.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10646..10873
                     /locus_tag="LZB08_001460"
     CDS             10646..10873
                     /locus_tag="LZB08_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBM7314967.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10878..11264
                     /locus_tag="LZB08_001461"
     CDS             10878..11264
                     /locus_tag="LZB08_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBM7314968.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11267..11971
                     /gene="atpB"
                     /locus_tag="LZB08_001462"
     CDS             11267..11971
                     /gene="atpB"
                     /locus_tag="LZB08_001462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBM7314969.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            12040..12300
                     /gene="atpE"
                     /locus_tag="LZB08_001463"
     CDS             12040..12300
                     /gene="atpE"
                     /locus_tag="LZB08_001463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBM7314970.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12423..12920
                     /gene="atpF"
                     /locus_tag="LZB08_001464"
     CDS             12423..12920
                     /gene="atpF"
                     /locus_tag="LZB08_001464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBM7314971.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12917..13462
                     /locus_tag="LZB08_001465"
     CDS             12917..13462
                     /locus_tag="LZB08_001465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBM7314972.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13479..14981
                     /locus_tag="LZB08_001466"
     CDS             13479..14981
                     /locus_tag="LZB08_001466"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBM7314973.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            15009..15887
                     /gene="atpG"
                     /locus_tag="LZB08_001467"
     CDS             15009..15887
                     /gene="atpG"
                     /locus_tag="LZB08_001467"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBM7314974.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15900..17294
                     /gene="atpD"
                     /locus_tag="LZB08_001468"
     CDS             15900..17294
                     /gene="atpD"
                     /locus_tag="LZB08_001468"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBM7314975.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17297..17557
                     /gene="atpC"
                     /locus_tag="LZB08_001469"
     CDS             17297..17557
                     /gene="atpC"
                     /locus_tag="LZB08_001469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBM7314976.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            18019..18399
                     /locus_tag="LZB08_001470"
     CDS             18019..18399
                     /locus_tag="LZB08_001470"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBM7314977.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            18414..18875
                     /locus_tag="LZB08_001471"
     CDS             18414..18875
                     /locus_tag="LZB08_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBM7314978.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            18896..19486
                     /locus_tag="LZB08_001472"
     CDS             18896..19486
                     /locus_tag="LZB08_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7314979.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            19501..20658
                     /gene="alr"
                     /locus_tag="LZB08_001473"
     CDS             19501..20658
                     /gene="alr"
                     /locus_tag="LZB08_001473"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBM7314980.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            20691..20978
                     /locus_tag="LZB08_001474"
     CDS             20691..20978
                     /locus_tag="LZB08_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBM7314981.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            20947..21330
                     /locus_tag="LZB08_001475"
     CDS             20947..21330
                     /locus_tag="LZB08_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBM7314982.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            21501..22328
                     /locus_tag="LZB08_001476"
     CDS             21501..22328
                     /locus_tag="LZB08_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBM7314983.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            22321..23262
                     /locus_tag="LZB08_001477"
     CDS             22321..23262
                     /locus_tag="LZB08_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBM7314984.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT         9274 a         2560 c         4554 g         6900 t
ORIGIN      
        1 tgcatatttt aaaggaaaat attattataa gatataaata tagtgataaa tttataaaaa
       61 cgtgctaaga ttggacttga aatacagtaa tatagtaggt aataatgttt taaattttat
      121 ttattgacca taatatataa ataatgatat tattagactt aactgtttaa ataaaacttt
      181 tctttttcat gccttcaatt tccaaaataa gtatgtatat aaatgaatat atagatgagg
      241 aggatttttt tgattttaga tgataaagta ttagaaggat taggtagtaa gaccttagtt
      301 gagctacgag agatagctaa agaattaaaa ataaaatcaa ttactacata taagaaaaat
      361 gagttaattg agattataaa ttccaaaagt aaaaatgagg aaaaagtaaa cgaagagact
      421 aaagaaaaaa atgaagatat taaagaagac ataaagaata gaaaagaaaa tatagagcgt
      481 gaaagtatag aaaaagaagt tgaatataag agccctacca aatcatacaa caagaaagaa
      541 atagataccc aaaatatttc ccaaaaccaa gaacatagaa atcaaagaaa tgactataat
      601 aagacaacga gtgtaaatga tagtaataag tcatcaaata tatctaatag aatggtcaga
      661 aataataata aaaactatta tatgcctaaa caagttgatg aatctaagat agtagatgaa
      721 tttaacacat caaaagagga tgaagtagta ggtgtacttg aaattctgcc agatggattt
      781 ggttttttaa gaggttcaaa ttatttgtct actgaaggag atgtttacgt ttcaccatct
      841 caaataagaa gatttaatat gaaaacagga gacaagatta agggaataac tagacatcca
      901 aaaagtggag agaaatttag agcactttta tatgttcaaa aaataaatga tgaaaaccct
      961 gacacagcta tacaaagaaa tgcgtttgag acattaacac caatatttcc agaagaacga
     1021 cttacactgg aaactaatag aaatgaaata gctacaagaa ttatagattt aatttcacct
     1081 ataggaaaag gacaaagagg attgatagta gcacctccaa aagctgggaa aacagttctt
     1141 ttaaaaagcg ttgcaaatag catagctaaa aatcatccaa atgttgaact tatagtactc
     1201 cttatagatg aaagaccaga ggaagttact gatatgaaag aatctattga aggagacgtt
     1261 atatattcca cttttgacca agtatcaagt catcatgtca aggttgcaga gatggtgtta
     1321 aatagagctc aaagattagt tgaacatggt aaagatgtag taattttact agatagtata
     1381 accagattgg caagggcata taaccttaca atatctccta caggtagaac tttatctgga
     1441 ggtattgacc ctggtgcact tcatggacct aaaaagttct ttggtgcagc aaggaatatt
     1501 agacagggtg gttctttgac aatattagga acagcattgg ttgaaactgg ttctaggatg
     1561 gatgatgtaa tatttgagga atttaaaggt acaggaaata tggagctaca tttagataga
     1621 aaattagcag aaaaaagaat attccctgca atagatattt acaagtctgg tacaagaaga
     1681 gatgatttat tgcttgatga tgaagaaaag actgctcttt ggagattaag aagagaaatg
     1741 agtaataatt cagtaatgga aattacagat aaagtcattg aacttataaa gagaactaag
     1801 aacaataaag agtttgtaaa atcaataaag aatttgtaaa atcaataata caaatgcaaa
     1861 tatggattat ataaactaat agaaaaatca cttgaatggc ataataagtt gtgctataat
     1921 aatgtagttg ataatgtaaa aagaaagtta atataattaa tttttttaaa taaagagaag
     1981 aggtgactat aatgcaaaag gaaatacaac caaaatacaa tccagttgaa gtgcgttgtg
     2041 cttgtgggaa cacatttgtt gctggttcaa ctaaggacga aataaaagtt gaaatatgtt
     2101 cagagtgcca tccattctat acaggaaaac aaaagaacat agaaaaaggt ggaagaatcg
     2161 acaaattcaa gaaaagattc aaaatggact aatttttata aaagtggagt tggaggaagc
     2221 caactcctat tttattacaa aaaaacattc atattctact ttaaattgtc tttttgtatt
     2281 ataataataa atatgtaaaa ttgagtattc taaatacaat aaaatgaggt gagtggatgg
     2341 caaaacaagc tgtaggtggg caagctgtta ttgaaggtgt aatgatgcag tcaaaagaca
     2401 aaagagcggt agctgttaga aaaagtgacg gagaaatagc actaaaagaa gatagaataa
     2461 aaagttgggt aagagataag aatatagata agataccttt tgttagaggt tcttttgtta
     2521 tgatagatac aatgattcaa ggcataaaaa gcttaaactt ttcttcagag ttttttatgg
     2581 aagaagctga agaggataaa tttgatttat ttataaagaa aatttttaaa gataaggcaa
     2641 atgatataat tataatattt tcattggtca tagcaatgtt actttctgct ggattattca
     2701 tttttatacc tacattggta ggaggagctt tttctaaagt aatgcctaat gattttatgc
     2761 ttaatttaat agaaggaatc ataaggatag ctatattatt tgcatatata gtattgattt
     2821 cacgaagtaa ggatatagaa agagtctttc aatatcatgg agcagaacat aaatctatat
     2881 attgctatga aaatgattta gaacttacag tagagaatgc tagaaaattt aaaagattac
     2941 atcctagatg tggtacaaat ttcctattta tagttatggc tgtatcaata atattgtttg
     3001 cattttttgg ttggccaaat ccaattctta gaatatttat gagaattata tgtgtgccta
     3061 tagtagcagg tttatcttat gaggtaatta gagttcttgg gaaatatgat aatggattta
     3121 ctaaaattat tgcatatcca ggaatgatgt tgcaatattt tacaacaaaa gagcctgatg
     3181 atgagcaatt agaggttgct ttggaagcat tgaaagcagt agtagattag agaaaaggat
     3241 atttgttatg acgataaaag atataattat taaatattca gataaactaa aagatataag
     3301 tgatactcca agattagata cagagttatt gttacaaaaa acattgggtg ttgatagatt
     3361 atatattcac ttaaatctga ataaagagct aactgaagaa caaaaaacaa aatttatggg
     3421 ttttgcagaa gaaagattaa atggaagacc aatagcatat atagttgaaa atagggagtt
     3481 tatgggatta gatttttttg ttaaggaagg cgtattgatt ccaagaccag atacagaaac
     3541 tttagtagaa gaaataattg aaatatgtag agaaaaaaaa gatgtaagta tattagatat
     3601 aggaactggt tctggagcta ttacaataag tttagcaaag tacattgaaa attctaaaat
     3661 catgtctttt gatatatcag agacagcatt agaaatagct aaaaaaaatg ctattataaa
     3721 tgaagttggt gaaaaaataa agtatattaa ttctgattta tttacagcaa taagtgatag
     3781 taacataaaa ttcgatatca tagtttcaaa tccaccatat attaaaaaac aagatataga
     3841 aactctgcac aaacaagtta aagattatga accatataat gctcttgaag gtggagaaga
     3901 tggattagac ttttatagaa gaataacaga acaaggtaag aaatatttaa ataagtgtgg
     3961 aatattagca tatgaagttg gtcataatca agctgaagat gtaataaata ttatgaaaag
     4021 taatggatat aaaaaaatat acacaaagaa ggatattcaa ggcattgata gagttgttat
     4081 aggttacaat atatgatata atcaacaatt gatatgttac atttaatcga ggtgaaagta
     4141 tgttaaaaaa attagaggta ttggaagata catataaaga cttaagtgaa aaaataggtg
     4201 atccggatgt aataaatgat caaaaagttt ggcagaaata tataaaagaa catgctgact
     4261 tggaacctat tgttatgaaa tacagagagt ataaaagcgt tttagatagt ataaaagaat
     4321 caaaagaaat tttacaagaa gagtcagatg aagaattaag agaattagct aaaatggagt
     4381 tagctgagat ggaagaaaaa gtagcacctc tggaggaaga aataaaaata ctattattgc
     4441 ctaaagaccc taatgatgat aagaacgtta tagttgaaat tagaggtgga gctggaggag
     4501 atgaagcagc tctatttgct ggtgatttat ttagaatgta ctcaaggtat gctgaaagaa
     4561 gaagatggaa aatagaatta ttgagtgcaa gtgatactgg agttggtgga tataaagaag
     4621 tatcttttat gataaaaggt aaaggtgctt attcaagatt aaagtatgaa tctggagttc
     4681 atagagttca aagaatacca tcaactgaat ctggtggaag aatacacaca tcaacttcta
     4741 ctgttgcagt attaccagaa gttgaagatg tagaggttga aataaatcca aatgatttaa
     4801 gaatagatgt tttccgttct tcgggtaatg gaggacaaag tgtcaatact actgactctg
     4861 ctgttagggt aacacatata cctacaggtg aagtagtatc ttgtcaagat ggaaaatcac
     4921 aattaaaaaa taaagagcaa gctttaaaaa tattaaaagc tagactttac gataaagcat
     4981 tggcagaaca acacaaagat atagcagcag aaagaaaaag ccaagtcgga acaggagaca
     5041 ggtcagaaag aataagaaca tataatttcc ctcaaggtag aataagtgac catagaataa
     5101 atttaacttt atataagtta gatgcattct tagatggaga tatagatgaa atgatagatg
     5161 ctttaattac tgttgaccaa acagaaaaaa tgactgcaat ataagtaatg ttttaaagaa
     5221 gctgtttctt cctagaaatt attttctatt ttgaaacagc tattttgcat tttaggtcat
     5281 gttatcatat tctattgtat ataattgtta tatacaagta taggagggtt atatgatact
     5341 aaaagtaaca ataattgggc ttttggcagg tgttataggg acaggattag gaggagtaat
     5401 atcagccatt tttaaaagag aggtcgataa atatctaagt ttttttatgg gattatctgg
     5461 tggaataatg ttggctgtag tggtgtttga tttaatgaaa gaatccatgg acaaaatggg
     5521 gataatcaat acagttatat ttacgtttgt tggagctctc ataactatgt acataaaaac
     5581 aaaattagat gtgtctggaa atatggcatc tggatatctt atattcataa gtatattact
     5641 acataattta ccagaaggac tagccattgg ttcttctttt atgtcaactg aaagtttagg
     5701 tattacactt gctatagtta taggtctaca taatatacca gaagggttag ctatggcatt
     5761 aggcttagtt tgcaataaaa tgaaactaag taaagttatt ttgtttactg taatagctgg
     5821 attacctatg ggtctaggaa gttttttagg tgtttatttt ggaggagtat ttacttctct
     5881 tataggagta tttcttgcta cagcaggagg tactatgatg tatgttgtat tggaagaaat
     5941 ttttccacat tcaaaaagtg tgtattgtat aattggtttc ttgttaggta caatgattgt
     6001 aaattatata taaaattaaa atgaaagcag gcatacaaaa gatgaaaact attataagta
     6061 atattgatat aaataatatt gattatgagg aaataaaaat acaagcaaaa cttcttagag
     6121 aaggtaaaac agttattttt ccaactgaga cagtatatgg gttgggtgca aatgcattag
     6181 atgaaaatgc agtaaagaag atttatgaag ctaaaggaag acctagtgac aatccattga
     6241 tagtccatat atatgaaaaa gaagaagtat atgacttagc caaagatata agtgataaag
     6301 ctaaacttgt tatagaaaag ctttggccag gacctataac aattatatta aataaaaaag
     6361 atataattcc atacaaaact agtggagggc ttgatacagt tgcaataagg atgccttcta
     6421 atgttattgc cagggcaata ataaaagaag ctggtatacc tattgcagct ccttctgcaa
     6481 atatctcagg tcgcccatct ccaacaaaag caaaacatgt atatgaagag atgaatggaa
     6541 gagtggatgg aatagtttta ggaggagaca gtaattttgg attggagtca actgtattgg
     6601 atttaacaga agagactcct atgatactga gaccaggaag tataactaaa gaagttttag
     6661 aggaacttct tggagaagta aagcttgacc catcattaag taaaaaagaa gataatcaaa
     6721 aagctaaagc tcctggaatg aaatataaac attattctcc aaatgcagat gtatatataa
     6781 tatctggaaa aagagaaaat gtagcaacaa agattaatga tatgataaaa tctaatagag
     6841 acaaaggtct aaagacaggt gtaatgtgta ttagtagaaa ccgagagttt tatgatggag
     6901 aagtaatcga tttaggaaac tctcttgaag aagttgcaag taatctgttt gatgctctta
     6961 tagaaatgga caaaaagggt gttgatgtca tatattcaga agagttccct aagactggag
     7021 ttggacaagc aattatgaat agactactga agtcagcagg atacaaagtg ataaaggctt
     7081 aagagggagt tgtaaaatat gaatatactt attgtgtgta caggtaatac ttgtagaagc
     7141 cctatggctg aggctatact aaggaaagca attaaagaat ctggaagaag tattgaagaa
     7201 tattctatat catcagcagg tatatctaca gcaaatggaa tgggtgcttc tgaaaattct
     7261 atagaagtac taaaagaaat cggtatagac ttatcaaatc atagaagtaa agtaattact
     7321 aaaaaattaa ttgatgaatc agatataata ctaacaatga ctaaatctca taaagaaatt
     7381 ttaatacaag cagttccaaa atgcaaagaa aaagtgtata cttttaaagg gtttgcaaat
     7441 aaaaatgagg aagatatatc tgacccattt ggtggaaatt tggatattta tagaagcact
     7501 atgagagaaa taatgtattc tgtcaacgaa atagtaaaaa aaatatgaaa cggaggataa
     7561 acaaatgaag ataggattag gctgtgacca tggaggatat aatttaaaaa aagaaataat
     7621 aagctattta gaaagtaaag gtatagagtg tgtagattat ggaactaata atgctacaga
     7681 ttctgttgat tatcctgttt atggagaaat agttgcaaat tcagttataa ataaagaagt
     7741 agattatgga atattatgtt gtggtacagg gataggaata tctttagctg ctaataaggt
     7801 tcctgggata agatgtgcag ttgtatcaga tgtattttct gcaaaaatgt caaaagcaca
     7861 taatgatgca aatatgttat cattaggcga aagagtttta ggaaaaggtc tagcacttga
     7921 gatagttgaa gcatggatta atacggattt tgaaggtgat agacatgcta gaagagtaaa
     7981 catgattaaa tcaatagaag aaaaacataa taaataataa aaattacagt tttatcagga
     8041 ggaattatca aaatgagcaa agtggtagag acaaaccatc cattaataca acataaatta
     8101 actttaatga gagataaaaa tactggttct aaagacttta gagagctttt aactgaaatt
     8161 gcaatgttaa tggggtatga aataacaaaa gatataccat taaaagatgt agaaatagaa
     8221 actcctattc aaaaaacatc atctaaagta gttgcaggta aaaaattagc aataattcca
     8281 attttaagag caggtcttgg aatggtagat ggattagtga gcttaatgcc agcagcaaaa
     8341 gttggacatg taggactata tagagaccca gaaacattaa aaccagttga atattattgt
     8401 aagcttcctc aagacatagg agaaagagat ataatagtag ttgaccctat gcttgcaaca
     8461 gggggttctg ctgtagcagc tatagattta cttaagtcta aaggagctaa aagtataaaa
     8521 ttagctaatt tagtagctgc accagaaggt atagcagaag tgcaaaaata tcatgatgat
     8581 gttgatatat atgttgcatc agttgacgaa agattaaatg aacatggata tataattcct
     8641 ggtcttggtg atgctggaga tagattgttt ggaactaaat aaaaataaat aactataaat
     8701 atttattagg gactttctta tatagtgtat ataattttat gtaagaaggt tcttttttat
     8761 atattttttc tcaaaatagg aattattgaa ttgataaatt tctcgaattg agaataaaaa
     8821 gtttttacca attaaaaaag attgcttatt ttacaaggtt ttatagaata gtagtctatt
     8881 ttaatgtggc atgttagttg cttacgatta agattatagg tattaagtgt attagttaaa
     8941 ttgagaattg aatattaata atattattgg gagtggtaat acatgaagaa ttgtatagta
     9001 gtaagagggg ctggagattt agcaacagca gttattcata aattacacaa cagtggtttt
     9061 aaagtagttg cgttagaaat agaagagcca ttagctataa gaaggcaaat ttctttttgt
     9121 gaggctgttt atgaaaaaaa agtaactgtt gaaggtgtag aatgtatatt ttgcaatact
     9181 gaaaaagaaa tagaagaagt tttatctcaa cataaagtag ccttgattat agacccaaaa
     9241 ggagaaaaat tatctaaatt aaatccaatc gttttggttg atggaatact cgctaagaaa
     9301 aacttaggta caactaaaga tatggcacca cttacaatag ctttaggtcc tggattttgt
     9361 gcaggagaag atgtagatgt tgttatagag acaatgagag gtcataatct aggaagaata
     9421 ataactaatg gtagagcaga aaaaaataca ggagtaccag gagcaataaa aggtttttct
     9481 aaagagagag ttatgcattc acagtatgat ggcataatgg aaaatgtatg taagataggt
     9541 gatatagttg aaaagggaga cctactttgc tatattaata attatgaaaa aaaacatgaa
     9601 gttagagcaa caatatctgg agtccttaga ggtttattaa aggatggagc aaaggtaaac
     9661 aaaaatttaa aaatactaga catagaccct agaaaagaag aagttaagaa tgcttttaca
     9721 atatcagata aagctagatg tatagctgga ggggttttag aagccatact aaacaatggg
     9781 atttatcccc aaaattaatt tatattagat aaagctatta atattttatt aagatgatat
     9841 aataaattta atagattaaa gagagcatat gacgggtatt taaaatttaa atggtaaaaa
     9901 tgctcttttt ttattggaca ttcaattaac atgtgtatat aatataaaaa taagtattta
     9961 tttaggttga aatctaataa atatataata ccagtacata agcaaagagg agaaaagtaa
    10021 attatgagac catcatggga tgaatacttc atggaaattg cagaagtagt gaaaaagcgt
    10081 tcaacttgta ttagaagaca agtaggagct gtaattgtaa gagacaaaca aatattaaca
    10141 actggatata atggttctcc aagaaatctt gaacactgtg aaaatattgg atgtaagagg
    10201 caggaattaa atattccctc aggagaaagg catgaattat gcagagcatt acatgctgaa
    10261 caaaatgcga taatacaagc tgctcataat ggtattagtg tagatggggc aactttgtat
    10321 gttactacaa gaccttgtgt tttgtgtgca aaaatgtgca taaatgcagg tatattgaaa
    10381 attgtttatg aaggtgatta tccagatgat atgtctacag aattattaaa agaagcagga
    10441 atagaactca taaaatttta gtggataaaa tttataagac aataaatgtc aaattatgac
    10501 actgaaatac aatggtatca aatgattaaa agaaagtatt tgaaaaatca tcagattagt
    10561 agtaagatat attttataat taaataaatt tatgagatag attaattaca tgttatttat
    10621 tttataataa tgagggatac tatttatgag taaaagaaat acacatacag aaattgcaaa
    10681 gttgtttagt ttaataagtc aaataggatt gatgattgta atatcaatat taggatgtac
    10741 tttcttagga aaatttttag atagtaaatt gaatactact ccagttctga caataatatt
    10801 tttattgttg ggtgttggag gtgcttttag tggtgtttat aaaactctaa tagtatatac
    10861 acgaaggaag tgatatattg gatataaaat tacttaacca ggtaaggtca gtaacaaaag
    10921 gtatagtaat atttgatgta gttttaattg tgctaatgtt gataacatca acattaagta
    10981 aaccagtatt attaggactt atatttggct ctataatagc tatgttaaat tttagattat
    11041 tggcgatatc actggaaaaa tcagtgacat ttcctgctgg gaaggcacaa gcttatgcat
    11101 cagcacaata tgctgttaga ttatttataa tggcagttgt tctaattgca tcagctacag
    11161 taccacattt aaatataatc ggaacagcat tgggattatt gagcacaaag tttgtcatat
    11221 tatcaaaaaa ctttataaat aaattaaaga ggaaggaggc ataagtatga atcaagcgca
    11281 ttattatctc ataggaaact ttaagattag tgaaacaata ctagtaagtt ggggaataat
    11341 ggccttttta gcgatagcat cttatttttt gactagaaac ttaaaaaaag ttccaactag
    11401 taaagttcaa atattcttag aatttgcagt aggtggtctt gctaaaatgg ttacagatgt
    11461 tatgggtaaa aatacagtaa aaagaatgcc atatatgata ccatacatag gaagcttgtt
    11521 tttatttttc gcatgttcaa atttaagccc attacttgga ttaaggtcac caacaaccga
    11581 cttagataca actgctgcat gggcaatgat tacatttttt atgatttatt ttgcaggagt
    11641 taaatttaag gggttatcat actttaaaga attggctcaa ccacttgcca taatgttacc
    11701 aatcaatatt ataggtgagt tagctagacc aatatcatta agtttccgtc catttgggaa
    11761 tattttaggt ggaacaataa taatgggtct gttttatcaa cttttaggtt ttatttctag
    11821 tttaatacca ggattatcaa taccaattgg agaaatatta ataccagtcc cattacactt
    11881 gtactttgac ttatttgcag gattgttaca agcatatata tttataatgt taacaatggt
    11941 atttatatca agtgcaggag agttagatta agaaatcaac ttatattaag ataaaaataa
    12001 aataatttaa aaaattaaag ttttaaggag gaaataatta tggaaacagc aatagtagcg
    12061 gcagcatcag caataggagc agggatagca gttgcaacag gtataggtgc aggtataggt
    12121 caaggtatag cagcagctaa agctgcagaa gcagtaggaa accaaccaga agctaaaggt
    12181 gatataacat caactttatt actaggagtt gctatagcag agtcttcagc aatatatggt
    12241 ttggttatat caataatact tctattcgta aatccattct ttaaatactt aggaatgtag
    12301 atttagtcct taaatatttt gagataggtt tataggtaaa tcatctcaaa cttataatca
    12361 tgagatgaac tttatcatct cgtgattata agtagttatg atttatggaa aggaggataa
    12421 ttatggaaaa agcactagta ggtataacgt gggaatttgt gttccaaatt gttaatactt
    12481 ttataatatt tttacttctg agaaagcttt tattcaagcc agtattaaat ataattgaat
    12541 caagagaaaa tgatattaaa agtgatttgg cagaaggcga aaaagcaaaa aatgaaggtt
    12601 tagctctgaa aaaagaatat gagtcaaaaa tcaattttgc caaagatgaa ggtcaagaaa
    12661 tcataaaaca agcaactata agagcagagc agaaatcaga tgatatagta aatactgcaa
    12721 agaaagatgc tcttgatata aaagaaaaag caaacaaaga tatagaacaa gaaagacaaa
    12781 aggttataaa tgaaatcaaa aatgatatat ctaacatagc actacttgca gcatctaaag
    12841 ttattgaaaa agatttagat aaatctaaac atgaagaatt gatagaaaac tttataaaag
    12901 aggtagggga agctaaatga taaatgtaat agcaaataga tatgccgaag ccctatttca
    12961 attaggagaa gaagaaaatt caactgatgt cttattcaaa gaactagaaa aagtagtaga
    13021 tatgatgact aaagttagta aagatttcta caaagtttta aaatcaccat tagtttcaaa
    13081 atcagaaaag aaaaacttgg tagaaattat ttttagtaaa gaagtaagtt ctaacataaa
    13141 aaacttttta aaagttttag tagataaaga tagaatatca tatttagaag atatcgaatt
    13201 agcttataaa gaattattaa ataagaaaaa caatgtaatt gatggagtag caataagtgc
    13261 tattccaatg agtgaaacag atattaaaga acttgaagtc aaactttcaa ataaatacaa
    13321 caaaaatgtt actatagaaa atgttgttga caagactata ctagggggag ttctagttag
    13381 aattggaaat gaacaaatag atggcactgt gaaaactcgt cttgataaga tgaaagagaa
    13441 gctatctgaa gtaatatctt aagaatggcg gtgaacccat gaacttaaaa cctgaagaaa
    13501 taagttctat aattaaacag caaataaaaa attatgagaa taaagttgag ttgacagata
    13561 caggtagtgt tttaacagtt ggggatggta tagcgagtgt atatggatta gaaaaagcta
    13621 tgtctggtga gttgctagag ttcccaggtg aaatatatgg tatggcactt aacttggaag
    13681 aagaagtagt tggagctgtt atccttggag atgacagtga aataaaagaa ggcgatatag
    13741 ttaagagaac tggaagaata gttgaagttc cagttggaga agctttaata ggtagagttg
    13801 taaactcact aggtcagcca atagatggaa aaggacctat agcgtacaca aaaactagac
    13861 cagttgaatc tgaggcacct ggtataatag atagaagatc tgtttatgaa ccattacaaa
    13921 caggaataaa atcaatagat tcaatgatac caattggtag aggtcaaaga gagttaataa
    13981 taggtgacag acaaactggt aaaacatcta tagttattga cactatacta aatcaaaaag
    14041 gaaaagatgt tatctgtata tatgtagcaa ttggacaaaa acgttctact atagctcaat
    14101 tagttagctc tttagaaaaa ggtggagctt tggattatac aatagtagtt tcagcaacag
    14161 catctgaatc agcaccactt caatatatag caccatatgc tggagcagct atgggtgaag
    14221 aatttatgta caatgggaaa catgtactta tagtgtatga tgatttaagt aaacaagctg
    14281 ttgcatatag agaaatgtca ttgttactta gaagaccacc aggacgtgag gcatatcctg
    14341 gagatgtatt ctatttacat tcaagattac ttgaaagagc agctaagtta tctgatgaat
    14401 tgggtggagg ttcaatgact gccttgccta taatagaaac tcaagctggt gacgtttctg
    14461 catatatacc aacaaatgtt atatctataa cagatggtca aatatactta caaccagagt
    14521 tattctattc aggtgtaaga ccagcagttg accctggtat atcagtatca agggttggtg
    14581 gttctgcgca aattaaagca atgaaaaaag ttgcaggaac attaaaactt gcatattcac
    14641 aatatagaga acttgcagca ttctcacagt ttggttctga cttagatgaa gatactaaaa
    14701 agagacttgc tcaaggtgaa agaatcgttg agatattaaa acaaggtgag catcaaccaa
    14761 taaaagttga aaatcaagtt atgataatat atgctgttat aaataatcat ttagaggata
    14821 ttccgataga taatatagct agatttgaat cagaattgta tgcatttgta gataataatt
    14881 atccggaaat atcaagaaaa atattaggtg gagaagactt cactcatgat ttaacagatg
    14941 caataaatga atttaaagaa aagtttgttg ttgaagtata atccttttta aaaaaggagg
    15001 taaattaatt ggcaggagct ggaatcaaag caatcaaaac acgtatggga actgttgaaa
    15061 gtacaaagca aataactaaa gcaatggaat tagttgcatc ttctaagctt agaaaagcaa
    15121 aagaaaaggc tgaagattca agagaatatt ttaatacttt atacaataca gtaaaagata
    15181 ttgcccaaac aacacgtgga attagaaatg aatttttaaa agaaagagaa gtaaaaaata
    15241 aatgctatgt agtagtagct ggagacagag gacttgcagg tggatacaat tctaatgtat
    15301 taaaggaatc tgtctctcat atgaaaggta aaaaggaaac agtgattact gttggaaaga
    15361 aagcacatga atttttttct aaaagaaact acaatatagc aaaagatata aatagtgttg
    15421 aagcttgtgg atatgaagat gcgacagaaa tttcacaaac tattattgac ctgtataaaa
    15481 aaggtgaagt agatgaagtt tatattgctt atacaaaatt taaatctgct ttatcacaag
    15541 aagttgaaat tttgaaattg cttccattag tctttgaaaa agatacagac gaacaaggaa
    15601 tggcagtaaa agaagaaaaa caaaatagat caaaagtaca atatatacca tcagcagaag
    15661 cagttttaag ttatattgta cctcagttta tatcaggtac agtatatggt ggaattttgg
    15721 aatcttttgc atcagagcaa ggtgctagaa gaactgcaat ggaatcagcc acagacaatg
    15781 cgaatgaaat gctatctaat ttagagttaa gttataacag agcaagacag tcagctgtaa
    15841 ctcaagaaat aacagagata gttggtggag ttgaagctct aaaataggga ggttaggaaa
    15901 tggcaaatgt aggtaaagta gtacagattg taggagctgt actagatgtt aagtttgata
    15961 gtgaacaaag cctacctaac ttattaaatg cgttagtaat aaagttagga gataaagaaa
    16021 tagttgcaga agttgctcaa catataggtg atgatactgt aagatgtata gctatgagtg
    16081 caacagacgg acttgtaaga ggtatggaag ttgtagatac aggagggcca ataagtgttc
    16141 ctgttggaga tgagacttta ggtagaatat tcaatgttct tggaaaacca gttgatggga
    16201 aaccagctcc aaaatctgca cctaaattac ctatacatag accagcaccg gcgtatgatg
    16261 aactagaaac tacagcagag atactagaaa ctggtattaa ggtagttgac ttactagctc
    16321 catatctaaa aggtggaaaa ataggtctat ttggaggagc aggagtagga aagacagttc
    16381 taatacaaga gcttataaac aatattgcta agcaacatgg tggtatttct gtattttcag
    16441 gagtaggaga aagaacaaga gaaggtaacg acctttatgg cgaaatgagt gagtctggag
    16501 ttataaataa aacagctcta gtatttggtc aaatgaatga accacctgga gcgagaatga
    16561 gagttgcttt aactggactt acaatggcag aacattttag agatgagcaa ggacaagacg
    16621 ttttactttt cgttgataat atattccgtt tcacacaagc tggttcagaa gtttcagcac
    16681 ttctaggacg tatgccatca gctgttggtt atcagccaac attagctact gaaatgggtg
    16741 cacttcaaga gagaataaca tcaactaaga aaggttcaat aacatctgtt caagcagtat
    16801 atgtaccagc agatgactta actgacccag caccagctac aacattctct cacttagatg
    16861 caaaaacagt tttatcaaga caaatatcat cactgggtat atatccagca gttgaccctc
    16921 ttgaatctac atcaaggatt cttgacccta gtatagtagg aaaagaacat tatgaagtag
    16981 ctagaggtgt acaatcaata cttcaaagat ataaagaact tcaagatata attgctatac
    17041 ttggtatgga tgaattatct gatgaagata agttaatagt tgcacgtgct agaaagatac
    17101 aaagattctt atctcaatca ttcacagttg ctgagcaatt tacaggaaat ccaggtcaat
    17161 atgtacctgt taaagagact gtaagaggat ttaaagaaat tcttgaaggg aaacatgatg
    17221 acttaccaga gtcagcattc ttatttgtag gaacaataga agacgctgtt agaaaagcga
    17281 agggaagtat gtagtcatgg ccagtgaatt ttcattgaaa gttgtaactc caagtaatac
    17341 tttttatgat ggagatgttg aaatgatagt agttagaact acaaaaggag acaaaggggt
    17401 gttgaaaggt catataccat ttgtttcagg tatacctgta ggaactttaa agataaaaaa
    17461 agagggaagt tttaaagagg cgaaaatagc agaaggattc attaatgtaa ctcaagataa
    17521 aactgtcata ttgacagaat ctgctgagtg gatttaataa attaatttag agtataaaaa
    17581 gtcattttga ccaaaaaaac ttttgggtaa aatggctttt ttgcgttttg gtgttaataa
    17641 aataacgata aaattgtaag tttttagtga taataaattc acaaaatgtt taaatttact
    17701 gccaaattat gttgtaatta ataagataaa gaagatttag atagctttta tgtaagtgaa
    17761 aaagtcttca tataagaaat acatataaaa tttttataaa atttaaaaaa tttgcttagt
    17821 tgtgtagtat aataaataat ataatagatt tgtatataat attcattaca aataatttta
    17881 aaagttacag ttttattata atgattatta aattaagata agtaacataa actaaataat
    17941 aatgtgtatt atcgatagta aatttataaa ttcattatta attattgaaa gaagtattat
    18001 aaattttctg gaggaacaat gaatatattt gatattggcg tagatataat tgaaattgat
    18061 agaataagaa aggctgtaga caaaaataat aggtttttgg agaagatatt tacagataga
    18121 gaaatagagt attttaattc taagaatttt aaagcagaaa gtatagcagg taatttcgca
    18181 gccaaggaag ctataagtaa atctataggt acaggaatta gagtgtttaa ttttaaagat
    18241 atagaagttc tcagaaatga aatgggaaaa ccaatagtaa aaacttataa taatctagca
    18301 aaaatgtgca tagattataa tgttttagaa ataaaagttt caatatcaca ttcaaaagat
    18361 tatgctatag caaatgcgat aaccataatt aaggactaag gagtgattac attatgatgg
    18421 ataaaacagc aaaagaaata atgacaacag atgtaatagt agcaaaacaa gatgatagta
    18481 tagcagatgt agctaatatg ctaattgcag aaaaaatagg aggtctgcca gttgtagatt
    18541 cagagaatag agttgttgga ataatttctg aaacagatat tcttaaaaaa gaaaaatata
    18601 tagaagcacc tctctatata aatctattac aaggattgat tttcttagat gacttaaaaa
    18661 aagttgaaaa agatataaaa caagtagctg cttataaggt tggagaactt atgtctaaag
    18721 atataataaa ggttcatgaa gatgataagt ttgatgatgt agctaatgta atgattaaaa
    18781 aatcaataaa cagagttcct gtagtagatg atgataacaa acttaaaggt ataatttgta
    18841 gatatgatat aataaaagct ttatacaatg aataaactga agtaggaggt gtgatgtgag
    18901 aaaaaagtgg accatagtat gtattatgtt cttggcactg gttataattg taataggatg
    18961 ccaaaaacga cagtccacaa aagaagaagt atacaaagat tttcaaaagc agatttctga
    19021 tatgaattat tacagttgta aagctgaagt agaagtagtt ggaaataaaa gtccacacaa
    19081 ttatgtttta attcatactt ataaaaaaac cgataactat aaattagaag taatttcccc
    19141 aaaacattta aaagggaaaa gtatagaata tcaaggtgat aaaatattag ttaaaaatcc
    19201 aaagattagt gatgtagtgg agttacctaa tacaggaaaa aataatcaat atctttttgt
    19261 aggagacttt ataaaaaact atcttcaaaa tgaggagatg aaagtaaagt tatccaaagg
    19321 acatttggtt ttagagactt ttattcctgg ggataataaa tattttaaca agcaagtatt
    19381 atatgtaaat gctgacacaa aaaatcctga aaaaatggaa gtgttagata aagagggagt
    19441 gccaagattt acagtaaaat acaaagattt tgaatacaga aactaagggg gactaaagac
    19501 atgcaaaaaa taacagtgcc tacatgggca gagataaatc tagataactt aagatttaac
    19561 ttaaataata ttaaaaattt attagaagaa gatattaaga tttgtggagt aataaaagct
    19621 gatgcatatg gacatggtgc agtagaagtt gcaaaattgc tagaaaaaga aaaagtagat
    19681 tacttagcag tagcaagaac tgctgaagga attgaactta gacaaaatgg cataacactt
    19741 cctattttga acttgggata tactccagac gaagcttttg aagattctat aaaaaataaa
    19801 ataactatga cagtttattc tttagaaaca gcacaaaaga taaatgaaat tgcaaaatct
    19861 ttaggagaaa aagcctgtgt tcatgttaaa atagactcag ggatgactag aataggtttc
    19921 caacctaatg aggagtcagt acaggaaata atagaattaa ataaattaga atatatagat
    19981 ttagaaggta tgtttactca ttttgctaca gctgatgaag taagtaaaga gtacacttat
    20041 aaacaagcta ataattataa atttatgtct gataaattag atgaggctgg tgtaaaaata
    20101 gctataaaac atgtatcaaa cagtgcagct attatggatt gccctgattt aagattaaat
    20161 atggtaagag caggaataat attatatggt cattatccat ctgatgatgt atttaaagat
    20221 agattagaat taagaccagc catgaaatta aaatcaaaaa tcggacatat aaaacaagtt
    20281 gaaccaggtg taggaataag ttatggacta aaatacacaa ctacaggtaa agaaacaata
    20341 gctacagttc caataggata cgcagatgga tttactagaa tccaaaaaaa tccaaaggtt
    20401 cttattaagg gagaagtgtt tgatgtagtt ggtagaatat gtatggatca aataatggtt
    20461 agaattgaca aagatataga cataaaagtt ggagatgagg ttatactatt tggagaaggc
    20521 gaagttacag ctgagcgtat agctaaagac ttaggaacta taaactatga agtgttatgt
    20581 atgatatcaa gaagagttga ccgtgtttat atggaaaata atgagcttgt acaaataaac
    20641 agttatttgc taaaataaaa ccatgatatt ataatctggg aggcgatttt gtgtacgata
    20701 agagtaaaga aaaaatagaa gttactttac ctgactctct gatttctgaa gttgatagta
    20761 tagttcagat ggagaacagt aacagagaag attttgcaaa agctgctttt cagttctata
    20821 taactcagaa aaagaaaatt catattaaag aatctatgaa aacgggttat agagaaatgg
    20881 gtcaaattaa tctgtcacta gctgaattag gtatgacagt tgaagaagta tcttctgatg
    20941 ataaaaatga agggaagatt gcccaaggtg agtactaatt gtgaaataaa gcgtggtgat
    21001 ttatattacg ctgatttaag tccagttgtt ggttcagagc agggaggcgt tagacctgtt
    21061 ctgataatac aaaatgatgt aggtaataaa tatagtccta cagttatagt atcagctata
    21121 acatctcaga ttaataaagc caaattacct actcatgtag agataagttc caatgaatat
    21181 gggcttaata aagactcagt aattctttta gagcaaatta gaactatcga taaaaagaga
    21241 ctgagggaaa aaattggttg tctagatgaa aatatgatga taaaagtaga tgatggactt
    21301 caaataagtt taggattgtt tactttttaa agagggattt gtcttttatt agacaatccc
    21361 ttttttcttt taaatatctc ttaaggttta attttaatgt actatgttaa taaaattaaa
    21421 aataacgcaa aaaaatagca taaaagtgat ataataatat ggaattgata taaaattacg
    21481 aactcaaggg aggtaattgc atgagagacc ataaaggatt aaatgagatt gcgcgtatca
    21541 taagaagaga tatagtttca atgatacata gagccaaatc tggacatcca ggagggtcat
    21601 tatcagtagt tgaaatactt actgcgttat actttgatga aatgaatgtg gattcttcaa
    21661 atcctaaaat ggaggataga gatagatttg tattatcaaa aggacatgct gcaccagcat
    21721 tatatgctac attggctgaa aaaggctatt ttgataagga agaactgaat ggattaagga
    21781 agataggaag gatgttacaa ggtcatccag acatgaaagg cacgccaggt gtggaaatat
    21841 ctactggttc tttaggtcaa ggtttttcag ttgcatgtgg tatggctatg gcatcaaagt
    21901 tggataatgc tccgtggaat gtgtatactt tgcttggtga tggtgaggtt caagaaggaa
    21961 ttgtttggga agctgctatg agtgctgcac attataaact tgataattta attgcttttt
    22021 tagataataa tggacttcaa atagatggag atatagaaag cgtaatgagt ttaggttcaa
    22081 tagttgataa atttaaagct tttggttgga atgtaattga gatagatgga catgattttg
    22141 accaaatatt tgcggctctt gatatagcta aatcaacagt taaaaaacct acaatgatag
    22201 ttgcaaaaac tataaaaggc aaaggaatat cttttatgga aaatcaagca ggatggcatg
    22261 ggacagctcc aagtgatgag gagctagaaa aagccttgct tgaattagga ggtgctgaca
    22321 atgagtaaaa tggcgacgag agaagcatat ggtaaagcat tagtaaaatt aggtcaaata
    22381 aatgacaatg ttgtagtatt agatgcagat ttatcaaagt ctacaaaaac acatgatttt
    22441 tacaagtcat ttccagatag attttttaat atgggtatag ctgaacaaaa tttaataggc
    22501 gctgcttgtg gattatcaac tgctggtaaa ataccttttg caagtacttt tgctatgttt
    22561 gctacgggaa gggcttttga gattataaga aattcagttt gttatccaaa gctaaatgta
    22621 aaaatatgtg ctactcatgc aggtcttact gttggagagg atggagcatc tcatgaaagt
    22681 gtggaagata ttgctataat gagagctata ccaaatatga cagttcttgt tccagctgat
    22741 ggagttgaaa cagaaaaaat tatttttgaa atagctaagt ataatggacc tgtttatgtt
    22801 agattaggga gaagttcagt accagtttta tttgatgaag attataaatt tgaaatagga
    22861 aaaggtacag tcttaagaga aggaaaggat gtatcaatta tagcttgtgg aattatggta
    22921 aatgaagctc tattagcaca agaaaagttg caagaagaag gtataagtgc tagagttata
    22981 aacatgtcct cgataaaacc tatagacaaa gatttaatat tagaatcagc aaaagaaacg
    23041 aatgctattg taactgtaga agaacatagc ataattgggg gattaggttc tgctgtaagt
    23101 gaggtagtag gagagtcttg tccaaccatt gtaaagaaag ttggaattaa agatacattt
    23161 ggagaatctg gtactccaaa cgaattgtta aaaaaatatg aattaacttg tgatgacata
    23221 ataaagactg ttaaagaagc tattattgca aaaagaatgt aagagatata aattattcat
    23281 gtttgtta
//
LOCUS       DAFKMX010000047        22800 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFKMX010000047 DAFKMX010000000
VERSION     DAFKMX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.047"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            191..1027
                     /locus_tag="LZB08_001478"
     CDS             191..1027
                     /locus_tag="LZB08_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7314985.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1380..2957
                     /locus_tag="LZB08_001479"
     CDS             1380..2957
                     /locus_tag="LZB08_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7314986.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2950..4134
                     /locus_tag="LZB08_001480"
     CDS             2950..4134
                     /locus_tag="LZB08_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7314987.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPIGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEESEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     NL"
     gene            4152..4751
                     /locus_tag="LZB08_001481"
     CDS             4152..4751
                     /locus_tag="LZB08_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBM7314988.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4775..5245
                     /locus_tag="LZB08_001482"
     CDS             4775..5245
                     /locus_tag="LZB08_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7314989.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5603..6349
                     /locus_tag="LZB08_001483"
     CDS             5603..6349
                     /locus_tag="LZB08_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBM7314990.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6410..7084
                     /locus_tag="LZB08_001484"
     CDS             6410..7084
                     /locus_tag="LZB08_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBM7314991.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7452..8798
                     /locus_tag="LZB08_001485"
     CDS             7452..8798
                     /locus_tag="LZB08_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBM7314992.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQMTLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKSINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNSNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8825..10219
                     /locus_tag="LZB08_001486"
     CDS             8825..10219
                     /locus_tag="LZB08_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBM7314993.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDIKMHKPEVGDIISTIIKDKSILSAHYGGTWEKIGEKI
                     EFGITVHYYKLIEK"
     gene            10429..11865
                     /locus_tag="LZB08_001487"
     CDS             10429..11865
                     /locus_tag="LZB08_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBM7314994.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKDMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            12209..13153
                     /locus_tag="LZB08_001488"
     CDS             12209..13153
                     /locus_tag="LZB08_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBM7314995.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDQIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEGNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13322..14254
                     /locus_tag="LZB08_001489"
     CDS             13322..14254
                     /locus_tag="LZB08_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBM7314996.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            14359..14826
                     /locus_tag="LZB08_001490"
     CDS             14359..14826
                     /locus_tag="LZB08_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBM7314997.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15070..15924)
                     /locus_tag="LZB08_001491"
     CDS             complement(15070..15924)
                     /locus_tag="LZB08_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBM7314998.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16090..18054
                     /locus_tag="LZB08_001492"
     CDS             16090..18054
                     /locus_tag="LZB08_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7314999.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEVVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18054..18515
                     /locus_tag="LZB08_001493"
     CDS             18054..18515
                     /locus_tag="LZB08_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315000.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18518..18838
                     /locus_tag="LZB08_001494"
     CDS             18518..18838
                     /locus_tag="LZB08_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBM7315001.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18854..19954
                     /locus_tag="LZB08_001495"
     CDS             18854..19954
                     /locus_tag="LZB08_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBM7315002.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20074..22674
                     /locus_tag="LZB08_001496"
     CDS             20074..22674
                     /locus_tag="LZB08_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBM7315003.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNHNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPVDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         8938 a         2359 c         4038 g         7465 t
ORIGIN      
        1 aatatctttc aatgaaaata tgataaaata tgacaaatta attgatgaca ataaaaaata
       61 ttttgttatt gtagtattat agggtaaatt gcaaaatata tacgaaagta aaattttaaa
      121 tattgacatt aaattaaata tactatacta tgtaagtgtg atgagagaat ttttttagag
      181 gggatttata gtgaatataa aaaagatatt taataacaat gtagtagtaa gctccctaga
      241 agatggcaca gaaataattg tcactggtgc tggagtagga tttaagaaaa aagtaggcga
      301 tttgatagat gaaaatttga tttctaagaa atattttgtt caagacgatc aaagggacaa
      361 atacaatcaa atacttaata agacctctat tgaatacttt aaaatttcag aagaaattat
      421 agaaaaggct aatgaagttt taaatactca agtgaatgat tcaataatat tagccttaac
      481 tagtcacata gaatttgctg tccaaagaga aaaacaaggt ataaaattac caaacttaat
      541 attgaatgag acaaaacaac tttataggga agaatttgaa tttggattat gggcaattga
      601 tgaaattgaa aaaaggatag gaataaaatt gcctgaagat gaagcaggat atattgctat
      661 tcacataatc aatggtttgg aaaattctca aaaagatgaa ggtattaata tactagagtt
      721 ttcaaaacaa gttattcaga taattgaaga agtacatgga tttaaattgg atgtaaattc
      781 attaaattat actagactta taactcactt aaagtttttt gttcaaagga ttctccgaaa
      841 agaaacttac aatgatagtg atattgaaga tatgtataag ttagtgaata aaaattacaa
      901 taaacctaaa gtgtgtactg aagaaattgc agcattggta ttagacagat ttgaatataa
      961 gattagtaaa gaagaagaat tatatttaat gattcacata aataaaataa caaacagtgg
     1021 ttgttaggat tgtaactgat aaagcaggca aaacctaagt cattgagctt aactattaga
     1081 ttaagttttt tgatttaggt ttttttatta gatacaatca aaatacagac agtgactttt
     1141 aggattgtaa ctggttaagc aggcaaaacc taaatcattg aatttaacta ttaggttaag
     1201 ttttttgatt taggtttttt tattagatac aatcaaatac agacagtgac ttttaggatt
     1261 gtaactggtt gagcaggcaa aacctaaatc attaaactta attattaaat taagtttttt
     1321 gatttaggtt tttctataaa tttgattatg tttttagata aaaataggag gggaaaatta
     1381 tgaaaaaaaa taagttaggt ttatggaact tcttccaaat gttaggaaaa acgtttatgt
     1441 tcccaatagc actacttagt gtttctggga tgatgctagg acttggggca ggttttacag
     1501 accctaagat gattgaaatg gttccatttt tagggaatga atatgttaac ttagttttaa
     1561 acttcatgtt aacaatagga ttatttgcat ttaacaactt gggagcatta tttgcaatgg
     1621 caattccact aggattatta aaaaaagaaa aagagtttgg tgctttttca ggattagtag
     1681 gatttatagc aatgcatata ggtacaaatt tttatttaac aagagctgat ttgttagtac
     1741 cagcagaaca gatgtcaact aatggtcaag ctatgattat gggtatacaa acatataata
     1801 caagtgtttt aggtggtatt gtagcaggat taattgttta tgcaatatat gataaagtta
     1861 gtaatttaaa gataccagaa tcattaggat tctacagtgg accaagatta gttccaattg
     1921 ttagtttaat agttatgagt atagttggat tattaatacc tattgtttgg ccaccatttt
     1981 tcaatgcatt ccaaagttta ggaagatgga tttcttcagc aggaccatta ggatattttt
     2041 catatgcagt ggcagaaaga gttacaattc catttggact aaatcatctt gtaacatctg
     2101 tattccgttt tacaccaata ggtggaactg ctgttattga tggagaaacg tactttggta
     2161 cattgaacat gtttatggca tatgtagaaa ataatcagat tatacctctt gatttagctg
     2221 gtaaaatgga acaaggtaaa ttgatgattc aatatggatt agctggtgct gcattggcta
     2281 tctaccgtac agcaaaacca gaaaatagaa aggcacttaa aggactttta atatcaggag
     2341 tgttgacagt tattataggt ggtataagtg aaccaataga gtttctattc ttattcatat
     2401 caccagcatt atttgctttc catacattta tgaatggtct agcaaacatg gtacttccat
     2461 acttaggcgt gttgatggga ttcacaggag atttaattgc atttataagt tttggtgtgt
     2521 taagaggaac agctacagga tggccaatag cagtattagt agcagtttta tattttgcaa
     2581 tatactattt tgtatttaag tgggcaatta ttaagtttaa tataaagacc ccaggacgtg
     2641 aggataaaga aataaaagta tctgaaggaa atggttcaga tttcaaaaat ttgtctacat
     2701 ataaaggaca acaaatgata tctgctttag gtggtcaatc taatatatta tcactagata
     2761 attgtgtaac tagattacgt ttaaaactac aagatgtatc attaataaat gaggatgcaa
     2821 ttaaagaggc tggtggtata gcagttgtta aattagatga gcatacaata caagtaatta
     2881 taggaactca agtgtattct ttaagaaagc agatggataa agctatggaa ggtcatttgg
     2941 aggcatgtaa tgattaattt tgataaaaaa gtagaccgat tgggtacata ttgtacccag
     3001 tgggactatg tagaagatag atttggtaag aaggatttgc ttccttttac aatttcagat
     3061 atggatttag agtttccatt tgaaataaca gaggtgttgc aggaaaggtt aaatcataga
     3121 gttttgggat acagtagatg gaaacatgat gattttagaa attcagttac gaattggtat
     3181 attaaaagat ttaattgtag cgttgatagt aactggatat attatagtcc aagtgtaatg
     3241 tattcaatta gcaaacttat tgagatgtta agtaatgaag gagatggaat ccttataaat
     3301 actcctgcat acaatgcttt ttatgatgtg ataacaaata acaatcgtaa tattataaag
     3361 tcacatcttg taaataggaa tggattctat tcaattgatt ttaatgattt tgagaaaaag
     3421 tgtaaaaaat ctaagatatt tattttatgt aatcctcaca atccaattgg tagagtatgg
     3481 agtgagttag aacttgttaa gatgataggc atttgtaaga ataatgatgt caagataata
     3541 tcagatgata tacacatgga tattgtatat gacaatagaa tgacacctat cttaaatgtt
     3601 gcaaaggact atttagattc tgtattcatt tgtacatcag cgtctaaatc tttcaatata
     3661 ccagcattga ctgggtcata tgtgattata ccaaatgaag gtattagaaa taaatttgaa
     3721 aatatcacta gataccgtga ttttgtaaat tcgccagcaa ttctagcaat attagccaca
     3781 atggtctgtt atgatgactg cgaatattgg ttagatgaac tattaaaata ccttaaagag
     3841 aatcttaaat atacgattga ttatataaaa gataatttag aaccattgaa attggtcatg
     3901 ccagaaggct gttactttgc ttggatagat ttttccgaac ttaaaataag tggtgagaaa
     3961 ttccaaaaat tattgatgga tattggagaa gttgctatta tgtcggggtc tgtatatggt
     4021 gaagaaagtg agttttattt aaggttaaat gtggcatgtt caagagaaaa acttgaagat
     4081 ggtttaaata ggattaaaaa aactatggat tatattgcaa aaagaaattt atagtaggaa
     4141 gggagatagt tatgggatat ttaaaaacaa tggttgatgt tatgactact gaaatgaatg
     4201 ccataagatg tttaatagat gaagcaggaa tagaatatga aagtatagtt agtgaaattg
     4261 caaattgtga aggcaaagta atttttatgg gtgtaggaaa atctgcacat ataggtaaga
     4321 aattggcagc tacatttgct agtactggta cacctagctt ttttgtacat gcaacagagg
     4381 cagtgcatgg agatttagga atgattgaaa gtaaggatat tacaatttta atatcaaata
     4441 gtggaaattc tatggaagtg gtaaactgta taaaatatat taaagcaata ggttctaaga
     4501 caattgcatt tacttcaaac agaaattcag tactggcaaa agagtgtgat tatgctttaa
     4561 tatatcctgc aaaggatgaa gcagatcatt taaatctagc accaacaaca tcatctacaa
     4621 ttactttggt tttaggagat tctattgcat gtgcattatc taagagtagt aattttggtt
     4681 cttcagattt ttataagtac catccaggag gaagtttagg tgagaagtta aaaacagcta
     4741 acaatggata aaagcaaaaa ggagagtagt ttttatgttc aaaatgttta aaaaaaaatc
     4801 aataaaatca cccatatcag gaaaagtaat agagttatct aaaatacaag atggtgtatt
     4861 ttctcaaaaa attatgggag agggtgtagc tatagattct acaggagaca ttgtatatgc
     4921 tccaatcaat ggaaagatag tagtagtagc agagactaaa catgcatttg taatagaatt
     4981 agaaaatggg atggaacttc ttattcatgt tggtttagat actgtaaatt taaaaggcga
     5041 aggttttgag gtattggtga gtatgggaga acaagtgaag gaaggaacac caatgttgaa
     5101 aattaaccgt cctttgattg aatcaaaggg tatatcactt attactccag ttacaataac
     5161 taatcattca gagtacaata tgaatacatg taatgtaggc aatagtgttg aaggtgggaa
     5221 agatactgta attgagttta aataaagaac taaaatataa ggttaatatt tataaatacc
     5281 tataaactta tgttttataa gattgattgt atagatttgt tgtataaaaa agaactcttt
     5341 aattataaat taaggagttt tttttatttt gtaaatattg tttagattta tatagaaaaa
     5401 cacttagtaa taaatagaat aaataagtat ttatatagct aaaaatctaa atagttttgt
     5461 ttaaatatat ttttatattt tgtaatttca aattaataaa taattagatt tgataaacaa
     5521 aaaataaaaa tgttgattat gttgataaac tttaatataa tatatttatg aaaaatgtaa
     5581 actgtgaaat ggaggtagca acatgaaaat tgaaaatctt tctattgatg atattaaacg
     5641 aggctatata tttaatgatg atttaaattg ttatagttgc attaaatgta caaaaacata
     5701 tgaggtagga gaaatatata aaataggtaa tagattcttt gaagcttcaa aagctattga
     5761 acttcatatg gaattagaac atagtgatta tttagagcaa ttgcttaata gtgattcaaa
     5821 atacaacaca ttgactgaaa atcaaaaaca attatttcaa ttatttatta gtgatttacc
     5881 agataaggag atagcaaaac aggttggagt atccacatct actatacgcc atcaaaaatt
     5941 tattttcaga gaaaaagcaa aacaggctaa gttctattta gcactatatg aatctgtatt
     6001 tgaagataaa tctaataaaa gtagtactat aataccaatc catgaacatg ctcgaatggt
     6061 agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac attttggata catcatttga
     6121 aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa gaaaagaaaa aggtcgtaat
     6181 tttatctaag attttggaac aatttgaacg tgggaagaaa tattcagaaa aggaagttaa
     6241 tcagatttta gaatctatat atgatgattt tgcaacaatt agaagatatc taattgagta
     6301 tggttttatg agtagaaata aagaatgtac aaaatattgg ttaacataat taaaataata
     6361 aattttacta atgtgtaatg ttatttttat ctagggggag gagtcaaata tgaatgatga
     6421 attaaaatct attttataca ataaaggtgt agacattgtt cgctttgttg atatatctga
     6481 atttccaata aatcaaacac aaggtttttc taaagcaatt ttattttgta tagggttatc
     6541 aaaaaaattt attacagata tatacaataa cttacctaca gatagtgatg agtttttgga
     6601 aaaagaggag aaagtagaag aattagctga ctggatttca aaatatattc aaaataaagg
     6661 atatagagcg tattctcaat cggagaaaaa caatctagaa catggatatt ttgaaaaagc
     6721 ctatattaat ccagaaatgc aatcagggat tagtccttta ccacataaga caatagcaaa
     6781 tattagtggc attggattta tgggaaaaaa taatttgttt gtaacagagg aatacggatg
     6841 tgcttttagc atgtgtacag tacttacaga tgctccaatt tctgttgaaa gatatccttt
     6901 gattgattca aaatgtatgg attgcaatgt ttgtgtagag aactgtcctg caaaagctat
     6961 acatggaaat gaatggactt tacctggaaa aagagaatct attattgatg tttcaaagtg
     7021 tttttgtgta ttgaggtgta tgatgagttg cccatggtca ttaagatatg caaatcaaaa
     7081 ataactttac tagagaaggt attaaattga atattataaa atttttaaaa tgtaagatta
     7141 tataagattt ctcaaaataa aaatttgtat agaacactca gaaatgagtg tttttctatt
     7201 gggattaaat tatattgata caaagcttaa ctattttaat cattatataa aatttgtatt
     7261 aaaaaattaa gaataacttg gtaatagtga aaatataatg agattataat tgatttataa
     7321 tataactata atgatattgt aaaatgaaaa ttgtcatttc aaaattccca aaaagagtaa
     7381 aagtcacttc ggttatagtc aaaaaaattt ttctgtgaaa agaatttaga tttaaaaggg
     7441 aggaattaat tatggaaatt gaaactaaaa ctccggattg gattaatgta ctagaattaa
     7501 acagtaaaat agatattact gggaaatatg atgtaagtaa tatgatacaa gatattgtct
     7561 ctgataagtc actagaaggt tccattatat attttccaaa aggtaattat ttatttgaga
     7621 aaggtattaa aattagtcaa caaatgacac ttcaaggaga ttcttattat ggaggagata
     7681 atcaagtatc aaatttagat aaaaaacaat caatcatagg aacaaccaat tttataactc
     7741 gaggagtttc taatatgagt attattgcat tgacaggaac aagtcaatgt atcaaaagta
     7801 ttaattttta ttatgatagt catgatatag agaaaatacc gcctaaaaat gtatctgcta
     7861 ttacagaata tggagaaaca caaggactat ctcattttga acacctgttt atttctggat
     7921 tttcagggat agggattgaa attccatact attcaactgg caatgatata attgttagtt
     7981 catgtggtct tggtatgagg cttggagaga agtcaatgct ttctagtagc aagatttacg
     8041 aatgtaaaaa tggaatggag attactactg gtgttagctt aaataatgtg agaatagaag
     8101 aaataaaaga aataggtatt aataataaag gatttggttt taacctgatt atgaatctta
     8161 tggtcgacca atgtggatat tgtggattta tgtttgaaaa aatgtgctat agccagataa
     8221 ctgccagaat tactagatgt ggtcagtatt ataaaagtgt tgattataat gcttatgaga
     8281 atatggaaga tagaaaagaa aaagcttatt ccatttttta tggagatatt ttagaaagtt
     8341 gcaatattgt attgatgaat agcaatattg ataccttaga aagtgattca cttaatgaac
     8401 ataagatata tgtaatcaag gcaaatgaaa caaacaatgt aatgctaaca tgttatgctg
     8461 aagcagataa ctttatacaa tgtgcaaagg gcaacttgct gttagaaaat ggtgtaaata
     8521 cttataaatt ttataatggt gagatttgtt ctgtaggagg tgttcaaatt tctgatatag
     8581 atgaaaaaga tttaatcaaa ataatcgaga acactctgta tatagatgat aaaggtaagt
     8641 atttaatggt tcctagatta aaaaagaata ctgtaatata ttcagtacta gattctacag
     8701 aagaaataaa taaattacat ggtggaatat gggaacagat tgatgtaaaa gtattagctg
     8761 gggaatccat gtactattat aaaaagataa aagattaaat tatcaaatta ggaggtagtg
     8821 attaatggaa gagtctataa taattaaaga tataagtggc tggattaatg tccttgatta
     8881 tggaatttgt aatgatgaaa caaaagatgt aagttttaag ttgcaagaga ttatggatgc
     8941 tgtaccagaa gattctatta tatattttcc aaaaggaaaa tacctatttg aaagtggtgt
     9001 taagattaag aaacgattaa ctttatgtgg agattcttat attttggcga atgttcctaa
     9061 aaatactgct ggagtaacac aatttacttt taaaggaaag aaaaatccta aaagtgaaaa
     9121 tatagcaatt ataacagcaa aaagtgtaag acactgtatt aagagcattt gcttttattc
     9181 agatagttgt gatagagata tgacatctaa tcatgaacct ccaacagatg gaaaacctag
     9241 atttcatcat aatgtaatta taaattatag aaatatatcg gccattgtat gtgaagataa
     9301 acagggagaa tcaggtcatt atgaaaatct atcttttagt ggtttttctg gaattgcact
     9361 tgatatgcct aataattcta cagcaaatga tattacaatt tttacatccg gacttggaat
     9421 tagtacagga gaaaattcaa ctattgctaa cagtaaggtt tggggctgtg ttgatggaat
     9481 gataatttct actggaactt ttataaatgg tatgagggta gaagaaataa aaaaagttgg
     9541 aattaaaaat attggtagag gtttaaattt tattacaaat atcacaattg accaatgtgg
     9601 atattgtgga gtttggtttg attctatatc caatgttcag atatcaggaa atatcacaag
     9661 gtgtgggcag tactattatg atgtaagcta tgataattat ttaatgttgg ataatagagt
     9721 taaagaagca tattctttat tctatggtaa tttattggaa tcaagtaata tagttttatc
     9781 aaataccaat aaagataatt gggaagattt caaagaagat aagcataagg tgtatcttgt
     9841 agaagcactt gaaaccaaga atgtactatt aacatgcaat atagatgcta ctgactggat
     9901 tgttaagtca gaaaaaggaa atttaacgta caataatatg cgacaaactg ttatatttta
     9961 cgatggaaaa ctaagtagta tcaatggaat tggtatatct gatgaagatt atggggataa
    10021 agtcaagata aaaaaaggaa atatatatat aaatagtaag gatattaaga tgcataaacc
    10081 agaggtaggt gacattatct caacaattat taaagataaa agtattcttt ctgctcatta
    10141 tgggggaaca tgggagaaaa ttggtgaaaa aatagagttt ggaataactg tacactatta
    10201 taaattgata gagaaataag taaatacgta gtatttgaat agatgtattt tattggagag
    10261 ctaggactat agttttaatg taaatggttt ggtaatagca tggattgaat aatataaaaa
    10321 taatataaaa aattaatttg attatgtaaa attctatgaa gtagtaaaac tgtgacttta
    10381 gtcataggag gttcaaacta agtaaaaggg aaaggaatat ataatattat ggaaggaaga
    10441 actgggatta aagatggaag atggattaat gtagtagaaa gaggtattaa aaatgatggt
    10501 tcgtacaacg taggtcctgc tttgcaggat attattgatg aaatagaaac agatttttgt
    10561 gtcttatatt ttccaaaagg taaataccta tttacaagtg gtgttaaaat taataaagaa
    10621 attgtattgc aaggtgattc taattcatct acaggcacta cacaatttat aactcgtgga
    10681 gtagaaaata tgagtattat tacattgaca gggaagaaac aatgtattaa aaatataaat
    10741 ttctattctg atagttgtga gatacaggtc aatgaagaac caccgacaaa aggtaatcct
    10801 aaatatcatt atgagatgat tatcaataag aatgaaaaga atgttgagat gaacaatgta
    10861 tcagcaatcc tgtatgataa cagtaaggat atgattaaag gtctaggtca ttatgaaaat
    10921 atttatataa gtggtttttc gggtacagga attaggatac catattactc aattgtaaat
    10981 gatattacag tttcatcctg tgggctgggt attgatacag gaatagacac aattatttct
    11041 aatagtagga tatctaagtg tcaaaatgga atgaggatta gaactggaac tagtataaat
    11101 aatgtaagga ttgaaaaaat acagaaagtt ggtcttataa gtagtcaagt taaggggtat
    11161 gaggggtatg gaagttataa aattaataat attacgattg accaatgtgg gtattgtggc
    11221 ttttcatttg attcaatgaa agatagttat ttttctggta ttataagaag gtgtggtcag
    11281 tattattata atacagatta tgacacatat ttaagtatta aagatagagt agaagaagca
    11341 tattctttat tttatggaaa ttattttaca gaatgtaata ttgatttagt tagtgataat
    11401 aaggataact gggatgatgg cttagaatat aaacataaag tttatgtatt taaaaccctt
    11461 caaataccaa aattaacatt gagatgcaat atagatgcct atgattatat cgttaaatcg
    11521 gtacatggag gaggtaattt aatgttaaaa aataacttta acaattatat atttattgat
    11581 tcatctagtc cagaagttat taatggaatt aattttaatt atcaaaagaa tggaaacctg
    11641 ttaaggttaa ataatggtaa attaagttat aaaaatggaa aggaagaaac agctatattc
    11701 agacctaatt atggtcttat tgtagcatca acagattcag aatgtgaaca aatagaggct
    11761 acctatggag aaaaatggga gaagcttggt tctgaagtaa ttggaaaaga aacggtatat
    11821 tattataaac atcatgaacc agaaaaaaca aaccaaatac aataaaattt gtattatata
    11881 atgatataaa gttttaatat atagtgttta cctttaatgt aatattgata tatttgtata
    11941 gtattaaatt tgttaaaaag taaaattgta gggtgcttga aaatagcacc ttttttagtt
    12001 tcattataaa attagttttg aaagtttagg aataatgtgt atttattttt aaagaattat
    12061 aaccttatta aggatatttt aaatgctaga aatgaatcag taaatcatac tggaattatc
    12121 ttaaatgttt tgattaaatt aagtatttag agctataatt atattaaaaa tatattagaa
    12181 cataattata ttactatgga ggttaaatat gatagtaagt gtgagtagaa gaactgatat
    12241 acctgctttc tatagtaagt ggttttttaa cagacttaaa gaaggttttg tttatgtaat
    12301 taatccaatg aatccaaaac aggttagtaa gatagagtta aatccacata ctgttgattg
    12361 ttttgtattt tggacaaagg atgtaactcc aatgttatat gatttaaata aattaaaaga
    12421 ctataaatat tattttcact atactataac atcatatgga aaagaagtag agacatgtat
    12481 tttagataaa agaaaggtaa tagattcatt taaggaattg tctagaaaaa taggcaaaga
    12541 aagagtaatt ttaagatacg accagatttt cttaagtgaa aagtacacag tagattatca
    12601 tatcaaagca tttgcgagtt tatgtaatca gcttgatgga tttacagaaa aatgtataat
    12661 aagttttatc gatttataca agaaaacaaa atttaatact aaagcattac atataaagcc
    12721 aatagaaatt acagaaatag aaacattatc aaaagagatt tctaagattg ctcataagca
    12781 tgggataaca cttgaaatgt gttctgaaga atataattta agtaaatttg ggattggaaa
    12841 aagtaagtgt attgatgata gattaatatc aaaaattata ggatgtgaag tagaggtaaa
    12901 aaaagatagt acacaaagag atatttgtgg atgtgttaaa agtgtcgata taggacaata
    12961 caatacttgc agacattatt gtctgtattg ttatgctaac tttaattatg caaaagtaga
    13021 agaaaactgt aggctttata aagaaggtaa taaattactc atagatactt taagagatga
    13081 tgcaaaaata agcattagag atatgaagac tattaaagta gataagaata aacaaattag
    13141 tatatttgat taaaataaat atactaatta ccttaatata ttaagaatat agatactttt
    13201 gataacaaaa ttaaacttaa tagatatttt ctatataaga aagatggtca taatatgtca
    13261 atttgaccat ctttttgata taattaaaac acaaataatt tagtaacaaa ggaggcagaa
    13321 cttgaattta ttaaaaaaat atcctatacc tattgcaagt ttgattttat caatttttac
    13381 tttaggtaac ttattacaat cttatagtga atcattacgt aatgtaattg gatttatagg
    13441 atttattttg tatgcaattt atatagtcaa aataatatta cttagaaagg gtgttaaaga
    13501 gcaattagag aaccctttaa tatcaagtac atttccaact ataacaatgg caacaatgat
    13561 atttgctaca tatataaaac cattatcttt agaattaagt attatgattt ggagcatagg
    13621 aattgtgggt catcttgtgt taatagtctt atttagtaag aagttcttag ttaaattctc
    13681 aataaaaacg gtatttccat cttggtatat agtatatgtt gggatagttg cagctagtgt
    13741 tacggcacca gcatttaatc aacttttgat tggcaaaatt gctttttgga taggatttgt
    13801 atcttatata gttcttatac cagtaatttt aaagagagta tggataatca aggaaatgcc
    13861 tgaaccatct ttaccaagta tagctatatt agctgcacca gggtcactat tattgtctgg
    13921 atatattaat tctttttcag ataaaaataa tgtagtatta tacttgttat tcatattatc
    13981 aattgtattt tatattatag ttttgggata cttaccgaaa ttattaagat tgccttttta
    14041 tccgagtttt gctgcattta cattccctat ggcaataagt gcaatgggta tgaaactaat
    14101 gaatggttat ttaactaaat caggtcattt tataagttgg atttcatatg cttctaagtt
    14161 agaagagata attgctacag tgattatttt atatgtttta gttttgttta ttatatatct
    14221 tagtaaatct gaaactacat ctaaagttaa ataagaatta ttaaatatgt gtatttaata
    14281 ggaatattga tataaatata tgaagataag ataaaccata gaaacttaat ttagcttaat
    14341 tttattggag gaaaacaaat gaaaacaaaa gaagaatatt taaaagaagt tagaaacata
    14401 tcagaagaat actttagaaa aggagagttt ttttgctctg aagcagttct ccagacaata
    14461 aatgatgctt taggccaacc tttaagtcca gagataacta agttagcatc aggatttccg
    14521 ataggacttg gaaaggcaca atgtctttgc ggagcaatat ctgggggaga gatggcttta
    14581 ggtatagtat atggaagagt tcatggtgaa gctatgaatc caaagatgtt tgagcatgct
    14641 aaaggtttgc atgattatat taaaaaagag tatggtgcaa catgttgtag agctattaca
    14701 aagaagtggg atggtgataa ctttatgtct cccgaaagaa aagagcactg tattaaaata
    14761 acaggtcaag ttacagagtg ggttgcagat aagcttatag aagatgaaaa acttaatatc
    14821 aaatagtgca atattagttg gtgaatttat aaagttaaat ttacttatag tataagatac
    14881 ttgatattct cgtattgata gaaaaattta tagcatatgt atttaatgaa atatacttat
    14941 atttagagtg tggtttaatt tcataaatac atatgctatt attttaagta tataagcaaa
    15001 tttatttaaa taaatagaaa cagttaattt atatgtacaa aactatatat gattagtgta
    15061 aataaatacc tattttataa tactcaacat aaagacacca gacatgatta gtattatacc
    15121 aataaaaata gcaagccatt cttttttgtt cttcttttct ttaaaaaata taatccctcc
    15181 taaagtggct actatcacac aactctgaga gatagaaaaa cttattgtaa ctcccaatgt
    15241 attagtcgtt atgaacatac ctaaatttgc tattgaccac aatataccag taattaaatt
    15301 gaataaaaca ttttccatat atacaatctc tttagaaaaa tacttgacaa taatagatga
    15361 agttatcaac ataccaacag attgaggtaa tataattcta tatccttcaa ttaaaaatat
    15421 ttgattagtt acaacatata atgataaaaa tagagatgat aataatacaa ctccaacaca
    15481 ttttaacata tttttatctg tagatttttt agtttgataa tttgtgagaa gtataccaag
    15541 tattatacat aatattgata aagaaccaat cagtaacatt ttagaactgg tccattcatt
    15601 aaacaagatt acagctaata atgtcgtaaa agttaattgt gaaccatttg aaattggcat
    15661 tactttagat acactagcaa tttgaattgc tttaaactgc attaattgtc ccagactcca
    15721 gaatatgcca gaaatcatac ttatacaaaa tgaaaaaaat gtaatttcag gatgtataac
    15781 cagagttatt atagtggaaa atataaacgc acttatggat gtacctaaaa gctgttcatg
    15841 aggagaactt ttctttagat ttgcaactat gggcatcaat ccccatccca ttacaggaag
    15901 taaagcaata attattgtca gcattaaact atttcctttc atattaaatt tagacagctt
    15961 ttaatttatc atctaaaatt aaagtgttca atacataaaa attaccgtat ttaatcagga
    16021 aatattagta gttttttaga aaaacatttt ccattaaaat aaagacaaag ggaaggagta
    16081 aaattgaaaa tgttaactga tatgattcaa aatcgtgaaa ttaaaataat aaagttttta
    16141 ttaaaagaag gtcatacaac tgtaaaagca atagcagatg atttagatgt ttcagaaaaa
    16201 acagtttcaa aatctcttaa agaagtagaa gtagtgctta aaaagatagg tctttcttta
    16261 ataagaaaac ctaagatagg tgtttatatt gatggagatt taaaaaaaat acttccttac
    16321 ctagataata aaggaagtca aattcctaca accagagagg agagagttat ctatatatat
    16381 tcagtacttt taaagagtaa tgattatatt actatacaac aactttctga tgaactatat
    16441 ataagtcgtg gaactattga aagggattta gcagaagttg aaaaactgct aaaaaacgaa
    16501 gggatttcca tatataagaa acctagtaag ggaataaagt taaatataag tgaaagagat
    16561 aaaagacaac ttactgctaa gtttatacat aaattttgga gtagaaactg gtatattaag
    16621 caagagcaag aaagctttta tcaagcattt gaaaagatac aggcagatgt aaatggtatt
    16681 ttttcggaag atgatttatc aataattatt gatattctta ggaatttcag cagagaaaga
    16741 aaatttgagt tctctgatta tgaatttcag tcattagtta ttcatttggc aatagctgta
    16801 gagagaatta gaaaaggcga atatatagaa gaaagtttgt atattggtaa tatagaaaat
    16861 aatcaaatgt caaataccaa ttatttagca aaacaattag aagaaaaatt ttcaatatta
    16921 ttaccaaaat ctgaaattgg atatataaat ttacatttag tagcgtctga ccaacataga
    16981 gatactaatg aactaaaaaa tacttcaact aatccttgtg agacaagtac aaataatata
    17041 tcaaatcttc gggaaattat aaaaaataca ttgtatgaga cagcttatga caaggattta
    17101 attgatggat taacttcaca tgtacattca tcaataaata gactgaaata tggacttagt
    17161 attaaaaatc catatgtaaa tacaatcaaa caaaactttt ctttatcatt tgagcaagct
    17221 ttagatttaa agaaagtaat tgaatttata tatgatatta ggataaatga tgatgaatgt
    17281 gcttatatag cattacattt tgaagctttt agagaaagaa tgaaagaact tccacaaaat
    17341 attagagtaa ttctagtatg cagtacagga ttaggttcat ctaggttatt gtctgcaaga
    17401 ataaaaaaat actttccaat gataaagatt gaaaaagtac tttccgtaca agaattagta
    17461 tccagtaaaa ttgatgcaga tttagtaata agtactattt atttagagtt agaaacaatt
    17521 ccaaccatag tagttagtcc tattttaaat gaaagtgata taagaacctt ggaaagaaat
    17581 atacaaaatg tttcaagttg tacttctaag aagtataaat ctttcagaaa cttaatattt
    17641 gaagaaaata tattgttgaa tattgaagct tcaaattatg aagatgcaat taagtatata
    17701 acagataacc taataaaaaa aggttttgca aaagaaggta tagctaaaag tgcaattgag
    17761 agagaaaatt tatcttacac gtctttccag tcaatagcta cacctcatgc taatcctagt
    17821 tatattaaaa aatctaatat atctgttgca acgttaaaaa atcctattac atggggaaat
    17881 gaaaaggtgg aagttatttt ctttatttca ttagaaaatg acaaaaacct agagcttgat
    17941 gaaatatatg aagtttttta tgacttcatt gacaataaaa aaaatataaa tgacatatta
    18001 agtgcaactt ccacaaagga agtgtacgat ttgctagtgg aggagagtat atagtgaaaa
    18061 tatcagatgt attgaaaaaa gagcaaattg tttttaatct tgatacaaat acaaaggaag
    18121 aaacaataaa aaaattaagt agtatatttg ttgaaaatgg gattgtggac aatgaagaag
    18181 aatatgttaa gtctgttttg gaaagagaag aacattcaac tacaggtatt ggtaatggta
    18241 ttgctattcc tcatgggaaa agtaatgctg tcaaaaaatc ttctataata tttgcaaaga
    18301 ctaagcataa gattgaatgg gatgctttag atgaaaaacc agtagatagt attttcttat
    18361 tggctataag caatattgat aaggatagca atcatcttgt actattatct aaattagcaa
    18421 ctaaacttat ggatgatgac aatgtagatg cactaaaatt agccaattca gaacaagaaa
    18481 taatcaatat atttagtgaa gaaggagaga tgtagaaatg aaaagaaaaa ttatagcagt
    18541 tacagcatgt gcaacaggag ttgcacatac atatatggca gcacaagcat taaagaaagc
    18601 ttcaaaaaag atgggtcacc ttataaaagt tgaaacacaa ggagctacag gaatcgataa
    18661 tgagttaact acaaaggatg cacaaattgc agaagtagta atatttgcag tagatactaa
    18721 agtcagaaat gctgaacgtt ttgagggaaa agaaatacta gaagtaccag ttaatgcacc
    18781 tataaaggat gcagagaaag taataaatga tgcattaaag ttaattgaca ctaaataaat
    18841 ggagggaaat gtaatgttga aaaagatagt ctctgagtta aaaaagcatg tcttaactgg
    18901 aatctcatat atgattccat tagtaattgc aggtgctatg ataatggcga tatctcgtgt
    18961 aggtggttct ttctataata taccagatat atgggatgct aaatatgcag aaagtgctag
    19021 tagtattgtt agattattac atgatttaga tgggtttgga ggtactgcat taggattaat
    19081 gtttcctgta attgctgcat ttataggatt ttctatagct gataaattag ctattgtacc
    19141 aggtcttgtt ggaggtatga ttgctaaaga tataggtgca ggatttttag gagctttggc
    19201 agttggtctt atagcaggat atacatgttt gtttattaaa aatcatgtta aattaccaaa
    19261 atcagcagct tcaatagtac caatatttat agtaccagta tttgggacat taattactgt
    19321 tgtattaatc aattatgtga taggtatacc ttttgccaca ttaaatactg gattagaaaa
    19381 ttggttaaat ggattatctg gtacaaatca gatactaatg gcagctatta taggggctat
    19441 gatggcagtt gacttaggtg gaccagttaa taaagcagct ttaacaacat cacttgcatt
    19501 attaacaagt ggaatttatg cgcctaacac tgctgcaatg gtaggaatag ttataccacc
    19561 actaggattg ggacttgcta ctatactagc aaaacaaaaa tataataaac agttacgtga
    19621 agctgggaaa tcatctctta taatgggttt aattggagta agtgaaggag caattccttt
    19681 tgcagtagaa tctccattaa aagtaatacc ttcatgtgta attggaacag caattgcttc
    19741 agctatggca gttggtttag gttcagtaaa tgcaactcca attagtggat tttatggatg
    19801 gtttacagta gagaattggc caatgtatgt gttatcaata gcagttggaa cagtaattgt
    19861 agctggatta gttatagtat tgcgtggaaa tccagtgatt gaggatgatg aatttgaaga
    19921 tgatgatttt gatgaagcag agtgggaatc ttaaaattta ataactattt aagacgtgtt
    19981 ataagctata ttcaatatga atcttatact aaataaagtg ttaaatattc agtttagact
    20041 taaatataaa agataaagaa cagaggagaa taaatgataa aagcacatat tgttaatcat
    20101 acacactggg atagagaatg gtattttact tctggagatg cattagtact tagtgaacaa
    20161 ctatttacag atgtaattga tgaattagaa cgtaatccag atgtaagttt tgtacttgat
    20221 ggacaacttt ctattttgga tgattatgta caattacaca aagaaaaaat agaaatcatt
    20281 aaaaagttaa taaaagaaga tagactacat attggaccat ggtttactca aactgatgca
    20341 ttctttgcta gaggagagtc tattttaaga aacttaatga ttggaatttt tgaaagtaaa
    20401 aagtatggta agtatatgaa aattgggtat ttgccagata catttggatt taatgcacag
    20461 atgcctatac tattaaagca tgtaggtctt gataatatat tcttttggag aggtatacat
    20521 cttggtaaac aggttcaatc accatatttt aagtggaaaa gcttaaatgg agaaagttat
    20581 atatatgcag taaatatgcc acatggatat ggaactggta tgcttttaga gccttcatta
    20641 aagtatgtta atggacgctt agacccagct attgatttta ttaaatcata tactgatgta
    20701 gatgaggtgt taataccttc tgggaatgac cagttaaata taataggtga tttcaaaaat
    20761 aaaattaaag cgattaatga tatcggaaag tattcatata taacgagttc atatcaagag
    20821 tttttaaaat atataaagtc catagaatca ctagaaagct atagaggtga atttagagaa
    20881 cctgtattag caagaataca taaatcaatt ggttctagta gaatggatat aaaattagca
    20941 tgtgataaac tagaaaacaa gctgattaaa agaatagagc cattattagt aattgctaaa
    21001 aagagtaaaa ttgaaataag caatcagtta cttataaata catggaaaaa acttttggaa
    21061 ggacaagcac acgatagttt agctggatgt gtaactgata ctgtaacaga tgatattctt
    21121 caccgtataa gagaagcaaa tgaaatatgt gacagtattg aaaatactat tgtaaaaaag
    21181 atttcagaag ggcttaaatt atctaaaaat gatattttag tatttaatac agaagctaag
    21241 cgttttaatg gatataaaga gatacaagtt gtaagtgata gtaaaaatat ttattttaaa
    21301 gataatttag atgctactat aattgaagaa acttatgtaa aaccgagaga aaatgtatta
    21361 gaagaaacac ctgctggcaa cgtatttatt gaagaaccag gatattatat attaaaagta
    21421 agaataaatg tacagcttcc tgcacttggt tataaagttg tcagttttga atcaagtgat
    21481 aaagaaatgg tatcacttga taaatctaat gacactttta tatctaatca taattataag
    21541 ataatctatg aagatggaag cttaaattta caacttaaag atggaactta catatcaaac
    21601 tttttaatat taaaagatag tggtaatgca ggtgatacct atgacttttc tccactaaaa
    21661 aatgatgagg atataaaact ttcgtttgat aaagtgtata cagaaaaagc tttaggatat
    21721 gaaaaaatgg tggttaaagg tactacttta ctaccactta cattagaaga tagaaaaaac
    21781 aaatatctta atggtaaatt aaatgtaaaa gtaagtattg tcttatctaa agaaaaccca
    21841 ttgatggatg taaaactttg tgttgataac acgatatata atcatagact acgtgttcat
    21901 ataaaaactg atataaaaga taataaaaac atagcatcat tgccatttgg atatataaca
    21961 agagaaaatg gagttttaga caactgggaa gatatttaca gtgaaatgcc tgtagattta
    22021 gaacctttgg aaagtaatat tacgcttaca aaccaaaata gaagttgtac tgtatttact
    22081 agaggaataa aagagtatca acatattgaa gatgaaattg cattgacctt acttgctact
    22141 acagatgaac taggaaagcc tgatttatta tatagaccag gacgtgcttc tggtgataca
    22201 actaaaaaag gtcatataag aattaaaaca gataaagctc aaatgcttaa gaaattagaa
    22261 ttttcatttg caatatatat ggattctaaa tcatttgatg aattggaaat tgcgaattta
    22321 acccacaatt atctaaggga atgtgtaaat tatcaattac aggattacaa tttcttttta
    22381 tatcgtatag ataataagat tcaaaaatca attgtcaata aaactgtaag tagagaatta
    22441 gaaataatat ctttaccaga aaactatctt atatcagcat gttatccttc ttattacagt
    22501 aaagataaat ttataataag agtggaaaat ccaagtagta atagaatgat attggatgag
    22561 caaatattta aaaatagaaa tgggaaaatt gtaaatgcaa tagaagatgt agaagaagaa
    22621 caggtatatg aaatagcacc ttttgacgtt atttcaatat tattggattt ataagtgtaa
    22681 aagtaagttt attgtttaaa gtctgacaga tattaatctg ccagactttt taatttgtat
    22741 aaatttgggt aaatattaat gaatggtttt agtatttaaa tatgaattta gtatttaata
//
LOCUS       DAFKMX010000048        22270 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFKMX010000048 DAFKMX010000000
VERSION     DAFKMX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.048"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..389
                     /locus_tag="LZB08_001497"
     CDS             <1..389
                     /locus_tag="LZB08_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBM7315004.1"
                     /translation="FDGGSIYRVEFKNSKLIGVRFDDCILKNVLFKDVLGKYSNFSFS
                     KFKIVSILDSNFENSVFQEVKGDNLVFEYTNLKKANFNGTKLSGIDFTTNNIEGIEIG
                     IDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            530..1801
                     /gene="brnQ"
                     /locus_tag="LZB08_001498"
     CDS             530..1801
                     /gene="brnQ"
                     /locus_tag="LZB08_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBM7315005.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2003..3190
                     /locus_tag="LZB08_001499"
     CDS             2003..3190
                     /locus_tag="LZB08_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBM7315006.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3420..5444)
                     /locus_tag="LZB08_001500"
     CDS             complement(3420..5444)
                     /locus_tag="LZB08_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315007.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            5834..6502
                     /locus_tag="LZB08_001501"
     CDS             5834..6502
                     /locus_tag="LZB08_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315008.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            6504..6989
                     /locus_tag="LZB08_001502"
     CDS             6504..6989
                     /locus_tag="LZB08_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBM7315009.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7050..8171
                     /locus_tag="LZB08_001503"
     CDS             7050..8171
                     /locus_tag="LZB08_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBM7315010.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFVALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            8452..9642
                     /locus_tag="LZB08_001504"
     CDS             8452..9642
                     /locus_tag="LZB08_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7315011.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENDTYRMDYDDLEE
                     KAKCNNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    9776..10031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10090..11091
                     /locus_tag="LZB08_001505"
     CDS             10090..11091
                     /locus_tag="LZB08_001505"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBM7315012.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            11195..11584
                     /locus_tag="LZB08_001506"
     CDS             11195..11584
                     /locus_tag="LZB08_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBM7315013.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            11745..12953
                     /locus_tag="LZB08_001507"
     CDS             11745..12953
                     /locus_tag="LZB08_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBM7315014.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(13039..14115)
                     /locus_tag="LZB08_001508"
     CDS             complement(13039..14115)
                     /locus_tag="LZB08_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7315015.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            14344..14871
                     /gene="hpt"
                     /locus_tag="LZB08_001509"
     CDS             14344..14871
                     /gene="hpt"
                     /locus_tag="LZB08_001509"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBM7315016.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            14960..15892
                     /locus_tag="LZB08_001510"
     CDS             14960..15892
                     /locus_tag="LZB08_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBM7315017.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNMSD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            15954..17180
                     /locus_tag="LZB08_001511"
     CDS             15954..17180
                     /locus_tag="LZB08_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBM7315018.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            17264..17485
                     /locus_tag="LZB08_001512"
     CDS             17264..17485
                     /locus_tag="LZB08_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBM7315019.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            17674..17844
                     /locus_tag="LZB08_001513"
     CDS             17674..17844
                     /locus_tag="LZB08_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315020.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(17937..18398)
                     /locus_tag="LZB08_001514"
     CDS             complement(17937..18398)
                     /locus_tag="LZB08_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315021.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            18581..19048
                     /locus_tag="LZB08_001515"
     CDS             18581..19048
                     /locus_tag="LZB08_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315022.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19091..20854
                     /locus_tag="LZB08_001516"
     CDS             19091..20854
                     /locus_tag="LZB08_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBM7315023.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            20862..21470
                     /locus_tag="LZB08_001517"
     CDS             20862..21470
                     /locus_tag="LZB08_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7315024.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            21542..22111
                     /locus_tag="LZB08_001518"
     CDS             21542..22111
                     /locus_tag="LZB08_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBM7315025.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         8455 a         2379 c         3825 g         7611 t
ORIGIN      
        1 tttttgatgg aggaagtata tacagagttg aatttaaaaa tagtaaattg ataggagtta
       61 gatttgatga ttgtatactt aaaaacgttt tgttcaagga tgttttaggt aagtattcta
      121 acttttcatt ctctaaattt aagatagttt ctatcttaga ttcaaatttt gaaaattctg
      181 tatttcaaga agtaaaaggt gacaatctag tatttgagta tacaaattta aaaaaagcaa
      241 actttaatgg aactaaactt tctggcatag attttactac taataatatt gagggaattg
      301 agattggtat agatgatatt agaggagcaa tttttgatgt gtcacaagca attgatttaa
      361 cgaaattgat gggaattatc ataaaatgaa aaaaatattc ataaaataaa ttgggaaaac
      421 gttaacaatg tgtggatttt taccagattg acatttttct gaaaataaag taagattatc
      481 ttaatattca aaatattttc aaagggggta atttagtgtg agtaagcaga tgagagatat
      541 ttttatagtt ggttttgctt tattttctat gttctttggt gcaggaaatc ttatgtttcc
      601 tccattttta gggatggaat ccggcaaaga ctggttaatt cctcttattg gatttgttgt
      661 tgcagatgct ggtatagcaa tgcttgttat tatagcaaca gcaagatgta agggaagcat
      721 ggatgatgta cttaggagag ctggtaaagg gttagcaaga gtaatgagtg tagctgctct
      781 agcatgttta gcacttcttg taataccaag aacttgcgca acaacatatg aaatgggtat
      841 tatgcctata tttggagacc atggtccagt agctaaagct gtattttcaa taatcttctt
      901 tggtctaact ttagcattta ctattaggtc ttctaaggtt atagacataa taggtaagta
      961 tttaactcca gcgttattat tagtattatt tgttttaata gtaaagggta taatttctcc
     1021 agcaggacct atgagtcctg agcatatgat agataaaaat ctatttggtg aaggtatatc
     1081 acaaggttat caaacaatgg acgctctagg tgcagcatct atggcaacta taatgttgat
     1141 gtctataata gctaaaggat atacatcaga taaagaccaa ataggaatga ctattaaagc
     1201 tggttttgta gcctgtgtat ttctagcagt agtatatggt ggtcttactt atttaggtgc
     1261 aacagtttca actttatatg atactagtat acctcaagca tctttattag ttgaaataac
     1321 tagtatattg ttatatggtc aagtaggaaa ggtaatttta ggtgtaattg tggcacttgc
     1381 ctgtctgact acttctacag gtttaacagc atcaatatca acttattttg aaggaataag
     1441 taaggcgagt tataaacaac tggttatagg tatatgtgta gctagtatgc ttatatcaaa
     1501 cttaggtgtt gattctataa tagccatatc agttccaatc ctacaaacta tatatcctgt
     1561 tttattagca atagttgtta tggagttggc tggtaaacat ataaagaatg ataatgcttt
     1621 taaaggtgca gcatatgtta cattagtgat tagtttatta tcagctataa atggaatgac
     1681 aggagctgtt ccatttatac aaagcttgcc acttgcaggt ttgggcttta actgggtaat
     1741 accagctata ttgggtggaa tagttggtaa ctttataaaa agcaacaaac aagtagcata
     1801 ataatttgta attaatttaa ttttataata tattaatttt tagtactatg ggtaggttta
     1861 atctaaacct aatctaccca tagtactttt tagattggat tataatagca tgtatataat
     1921 tttttataat ataatttttc tttgaagtat gataaaatta gtaatagtga taagaatgta
     1981 caaatttagg aggttttaat taatgcttag aaaaataaca gcatttatgc tatccatatg
     2041 tttaattatt ggacttacag gttgtagttt acttgtaggc aagaaagata aagagaaaat
     2101 tatagatgat tttaaagttg cagtggtaac tcaaccatta tctgaaaata aagtacaata
     2161 caatatggtg gaagagatgg caaaagaata tgaggaagag aataaaattg ataaagacaa
     2221 agatggtcaa actaaagtta aacaaactat aaagcatgtt gtattgccag agaattttac
     2281 ttctaatata gacagtgcta ttaataagat agtaaagttg gctgacgata aagaagttca
     2341 ggctattgta gtttcaactg accaagcagg gcttttacct gctttacaaa aggtaaagga
     2401 aaagcgtcca gaaataataa ctatttctgc ccctatggga gatgataaaa accaattaag
     2461 ccaatttgtt gatgtgaatc taggggttag tgcagaggaa agaggaaaag tattagctga
     2521 acgttcaaaa gaaatgggag caaaagcttt tattcattat gcctctacag atgatttgaa
     2581 agatgttaat atagccaaaa gattagaaat gattaaagag acttgcaaaa atataggatt
     2641 gccatttgta caagttaata ctccaaatat aaatactgaa gaagacaaaa acaaagttaa
     2701 gcagttttta aatgaagata ttgaaaaaca agttaaaaaa tatggaaaag atataaatgt
     2761 gtttggtgta aatgagtata tggatgaagt tatattgact aaggctttag aattaaagta
     2821 tatagttgca gagcaaagca atccatcacc aatacaaaca tatcctagtg ttatgggtct
     2881 aaaaatatct gaaaaagatg ctcaaaatta tgataaaata aacgacatga taagtgaaaa
     2941 ggctaaggct tttggcatgt caaatagact tggtggatat cctatgccaa tggatgcttt
     3001 tctaccatca gtagcaattt atcttgctac tgaaatggtg aaacaagatt taacacaaga
     3061 agatgtatgt gacccagatt atttagaggc gtttacagaa ttaagatttg gaatcggttc
     3121 agaatttact cctttaacag aggtacttta taattatcaa tcagtaattt taagccaatt
     3181 aatttattaa tattaaggac gtgtctcaaa atgaactttt tagtttatga ggcactcttt
     3241 ttctgttaaa tcaaatatat ttttatcttt ttaacaatta aaaagaagct tatccatatg
     3301 ttgagatagc ttcgttttta atataaataa gttttattat tttataaaaa atttatagaa
     3361 aaataaaaaa ttataattat atataaattt tgataaagta aagatgaatt actataggtt
     3421 taaattatat tattttctat tatttttatg cctttctcag ttattgatga gccttttctt
     3481 cccttactag aaattaagta tccattgcgt gtcaaatatt ctagcatatt tcttacttcg
     3541 tattctgtga cattatattc cataaatttc attaactttg tcctccccat tggtttcatg
     3601 tgcaatgatt ttaggataga aatgcatatg tttttgtcat attggtttaa atcattgaat
     3661 atggacatat tacttgacat atgattatta tttaattttg aagatatgtt ttgaggtaaa
     3721 tcatttattg taagataatt ggaacccatg taagccatat attcaacaca atttctcagt
     3781 tctctaatat tccccatcca agtgtatgaa tttaaagctt ctaaaagact cttatcgagt
     3841 tttatgtttt tatagggaag ttcttttaaa aatgattcta aaagaagagg tatatcttct
     3901 aatctctttc taagaggagg taaatctaaa gtgaacatac ttatcctata aaataaatcc
     3961 atcctaaatt tattttctat aattaatttc tctaaatctt tatttgtggc agctaatatt
     4021 ctcacatcaa ttggaataat acttgtacca ccaatttttc taatttcttt ttcttgtaag
     4081 actcttaaaa gtttaacctg taaatcatgt ggcatatctc ctatctcatc taaaaatata
     4141 gtgccagtgt gagctaattc gaataatcct ttttttccac ctttttttgc tccagtaaaa
     4201 gaaccatctt catatccaaa tagttcactt tcaagtaatt cacttggtaa tgaagcacaa
     4261 tttattgcta caaaaggttt attttttcta tttgaatgat tgtgtattga ctgagtaaag
     4321 gcttctttac ctgttcctgt ttcacctgtt attaatatag gattatctgt aagagccatt
     4381 ttttttgcct ttttaataca atcttttata atagaactat ttccaataat attattaaat
     4441 ttatatttag ctacataacc tcttttgaca gaatcacttc ttattttttg ttctaatatt
     4501 tctatattgt ttacatcttt taatgtaatt aagcaaccct ctatattttt ataaagataa
     4561 aatggttttt tagaaagtat gaatttttta tctatttcgt ctatgtatat aatcatatta
     4621 tcgatattta agttctttgt aattttatct aagaaactct ttggcatata ttccattaag
     4681 gaaggtgatt ttataagctt ttcatctagt tccaacatat taagtaaaga cttatttaca
     4741 aaagtaacct tgttaaaggt gttgaaaaat attagaccat cacctatttc atcaatggtc
     4801 atgtaaagta tagtttttaa tttagatata ttatctaatg atgtatttaa gaaatcatga
     4861 cttagtgtct ccttggacaa tttgtatagt ttttctataa atttctcatt ttttaatttt
     4921 aatactacta ataatgacat atatgtatct aatgagattt ttctccaacc taaatcaaca
     4981 atatttttta tgtattttgg tataaatcta atctgacctg gtgttatagc tgtatctaaa
     5041 tcatatattt catcacaatc tggatatgtc ggtacaaaat ctatatgatt tataccaatt
     5101 ttatatagat aagtagccat ctcaaatgct gaagttttat tggttgtcat aagtaacgat
     5161 tttgtacctg gaggcagttt catcattttt tcatatccaa gttttgaaag tgctttcgtt
     5221 ccatgaataa tttcaatatc ttccttaaaa tgttttatta aaaaattact agttaaaggt
     5281 gatagagaaa gcactacatc tccctctaaa ttttctttta ttccatcaag tatattttgt
     5341 gtttctatga ttatttcgtc tccaaaaaag tttgttaatt cttctttgta gtggtcacta
     5401 ttcaatttat catatgttat aaggattaac ttctttttat ccattttatt tctcctaaca
     5461 aaatagtatt tatttttaaa gtatttaaag tataaggcaa gttatattat acaacaaata
     5521 gtagtataaa tttatatatt tgattaaata ataggttttt ataggatttt atcctataaa
     5581 aacctatttt aaaattattt ttgcaagata aggaaaatag ctttttatat tgtaatgtat
     5641 attttaaata attaataaaa aaatattata tttttaaact tgaaaagtaa gtaattataa
     5701 gctatttaat cgctggaata aatttacaat aaaaaaataa atacaaaggc tttatcatta
     5761 aggtattggc ataagttttg cttattaaac aagtatacta ataaaattca tttaaacagg
     5821 agggagaatt tatatgagta aattagaatc aacaacagaa aaagttgagg taaaatggta
     5881 tgggtatgta gcattaatat tgggagcatt atttttttca ggaatattta aagatgcacc
     5941 aggaccatta aaagtattgg attttaacaa tgtattaggt tcttttggaa cattgggaac
     6001 aattaatgat ggtgtaggta cattagctgc aaactttaga ggtgatggag gtactggccc
     6061 tcgagatgga tggctatatg cattgacatt aataccatca gttatgtttg cacttggagt
     6121 agttagagtt atagaccact tagatggaat gaaggcagct caaaagcttc ttagtccact
     6181 attaaagcca ttattagggt tacctggatt tgcaggacta actttgatag ctagtttaca
     6241 gtctacagat gcagctgcat ctatgactaa agaacttaaa gatgatgggt atatagatga
     6301 aaaacaaaaa gctgtatttt gtgcatttca attttcaggt gcgtctgcaa ttacgaattt
     6361 ctttgcttct ggagcagcac tatttccatt tataggagat gtaccaatat ttataccact
     6421 tgctttaata ttaattatga agtttgttgg ggcgaactta cttagattat atttaaataa
     6481 atttgacaag ggggaagctt aacatgagtg aggtagcaaa aaaagaagta aaagttaaaa
     6541 aaggaaatgt gatagatgcg tttattggag gagctagaaa tggattccaa ataagtacaa
     6601 attcaatggc tccaaatgtc atcttcggtt ttgctataat ttctgtgttc aatttaacag
     6661 gtcttttgga tgtaataggg acaatattta cacctgtaat gagtatcttt ggacttccag
     6721 gtgttgcagc aacagctata atgacaatat ttatatcaat gggaggagca tctggagtaa
     6781 tcgcaggttt atttacagca ggtcaaataa gtcaagcaca tgttgcaatt ctattgccaa
     6841 tgttattgct ttcaggagca cagatacaat ttatggggag acttttagga acagctgagt
     6901 taaaaactaa atattatcca catatgtttg ttatagcaac tttaaatggt tgtattgcaa
     6961 tgcttataat gaattatttc gtagtatagg taattataat aaaaatttaa tataaatgga
     7021 aagtttaaat tttaaggagg agctagatta tgggaactaa caaagattat attgagtatg
     7081 tacataaagt gtatgattat ctacacacta taccagaaaa aggatttgaa gaatataaga
     7141 catcagagtt tgttttaaaa gagcttgaaa aaatggggtt tgaaaatatt aataaaggag
     7201 ttgcaggaac tggtattata gtaactatag attctaaaaa agaaggacca gtatttgcta
     7261 taagagcaga tatggatgca ttggaattta tcatagatgg agaaaagaaa atgatacatg
     7321 cttgtggtca tgatggtcat acttctatgc ttttaacagc tgcaaagtta ataaaagaga
     7381 gaaatttagt aaaaagaggt aagttaaaaa tactttttca accaggtgaa gaaaaattat
     7441 atggagcatt aagagtaatt gattctggtt tgttggatga tgtagaagaa atggtaggga
     7501 tgcatttaag acctggacaa gattgtaggt tgggaaaggc gatacctgca atgtaccatt
     7561 gtgctagctg tataatggaa tttacgatta aaggtatcac ttgtcatggt tctaaaccac
     7621 atttaggagt aaatgcagta gatgctgtct gtttagcagt gaatgcaata aatgcaataa
     7681 gagtaaatcc agctatacca aattctataa aagtaacaaa tatatcagtt aagggagaaa
     7741 cttataataa tataccagat gaagcatttg tggctttaga tataagggca gaaagtaatg
     7801 atataataga agaattaaaa gaaaaagcaa tacatgctgt aaaaactgct gtaagttcta
     7861 taaatgcaga agctgtacta atttccaaag atggagtacc agcagcagaa tttgatgaag
     7921 aacttgtaaa cttggctgaa gaaactataa aagatgtatt gggagctgat gcatctatag
     7981 gtatatttaa aaattctggt ggagaagatt tccattatta tacacaaaaa ctaaaatgta
     8041 aaactacata tattggatta ggagctgatg ctacaccagg atttcataat ccaaatgtaa
     8101 catttgatac gaaagcttta gaatatggtg tagatatatg gtgtagattg gttgaaaaga
     8161 gattgggata acatccaaac taataattct acatttaaaa ttataattat agaagttata
     8221 gaaaaagatt gcttatgtta attagaaaat aattaatatg agcaatctta ttttgatata
     8281 atgaaactga ttttggaata ttgaagaaat atgatgggaa agtaattata ttttaaatct
     8341 attggacaaa aaagtgatat tattataata ttaagaattg taaaataata ccaaagtgca
     8401 tctagtaata gacttaacaa ttataaaaat tttattctta ggagggaaaa aatgagtaag
     8461 tataattttg acagagtaat agatagagta ggaacagatt gtgtaaaatg ggattttaga
     8521 actaattgtt caacaaaagc acaaaaagat ggtttgcctt tttggattgc tgatatggat
     8581 tttgaatgtg cagaaccaat aatagaagca ttacgtaaaa aagttgaaca taaaatattt
     8641 ggatatagtt caaatgatag tgataaatac tttaatgcag tttgtgattg gtataaaagg
     8701 agatttaact gggaaataaa tagaaaagat attattttta gtcctggtgt tgtacctgct
     8761 gttgctattt tagtaagaat attaactaat tctaatgaag gtgtaatagt ccaaaaacca
     8821 gtgtattacc catttgaagc taagataaag agcaataata gggacgttgt aaacaatcct
     8881 ctgatatatg agaatgatac ttataggatg gattatgatg atttagaaga aaaagctaag
     8941 tgtaacaaca ataaagtact gatactttgt agtcctcaca accctgttgg aagagtttgg
     9001 agagaagatg aattaaaaaa ggttgtagaa atatgtaaaa aatatgacct atggataata
     9061 gctgatgaga tacattctga tttaattaga aaaggattta aacatactcc tttacagtca
     9121 ttatgcccag agtacaagga taaaatagtt acatgtatag ctccaagtaa aacatttaat
     9181 ctggcaggaa tgcagttgtc taatataata gtgaataatg atgaactaaa aaagaaatat
     9241 caagaagagg ttacagctgt tggagttgca acttcaccaa atccctttgc tattgtagct
     9301 acaatagcag catataatga aagtgaagac tggcttaatg aattaaatga ttacttagat
     9361 aataatatac aatttattga tgaatactta aaagaaaacc taccaaaagt aaaacttgtt
     9421 tatccagaag gaacatatct agcatggtta gattttactg cttatggatt aaatgaagta
     9481 gaacttgagg atttaatgtt taaaaaagct aatgtattat ttgatgaagg ttatatattt
     9541 ggaaaagaag gaataggatt tgaaagaata aatgtagctt gtccacagtc tttattaaaa
     9601 gaatgtatgg acagattaaa gactatattt gaaaatttat aaagtatagg taaaaattta
     9661 taaagtgttt aaaaataaaa aaataataaa cttgacaaat tttaactaat cacttatcat
     9721 actattatga ataaatagaa aacataagag agctaaattt tattgaatta aactcttaaa
     9781 gactttgaaa ggaagagtag ttatagaatg taattaaagc gagttggtga tggtgtgaga
     9841 ccaatatgaa gtctataatg aagaacatcc tggagttgct aaccgaaatt ttaaaagagt
     9901 aggcttagtc ggaaccaaac cgttatcata ttggggtgta tcgagtaata tctgtacatt
     9961 gtgagctggg tttgaattta tttcaaacta atcagggtgg taccgcggaa aggcaacctt
    10021 tcgtcccttt tatataggga tgaagggttt ttttaatgga aaaatatata aatataggga
    10081 gtgaatgata tgagcacaat aataccaaag gattataaaa gtagtttgaa cgtaattgat
    10141 acacaaatag ctataaaaaa attaaaagat ttttttgaaa tgagactaag taatgaatta
    10201 aatcttataa gagtttcttc tccgttattt gtattgccag aaacaggtgc aaatgataat
    10261 ttaaatggag aaaaggctgt aagctttgat ataccattta tgaataaaaa tgcagaaatt
    10321 gttcaatcac ttgcaaaatg gaaaagatta gcccttaaaa aatatgggtt tgaagttggt
    10381 agtggtttat atacagatat gaatgcaata agaaaaaatg aagaattaga caatatacat
    10441 tctctttatg tagaccaatg ggattgggaa ttggttatag ataaaagtag tagaaatgaa
    10501 aagactctaa aagatgttgt aaaaagattg tatggagtat ttaaagatac tgaaattttt
    10561 gtatgtagta tgtatgaagg tataaaagaa atattaccag aagaaataac ttttataaca
    10621 tcacaagagt tagaagatat gtatccagaa ttatcaccaa aggaaagaga agataaaatt
    10681 gtaaaagaga agaaagcagt atttataact caaataggaa aaacattaat atcaggagaa
    10741 aaacatgatg gaagaagtcc tgattatgat gattgggaat tgaatgggga tatattattt
    10801 tacaatcctg ttttagattc agcattagaa ttatcttcaa tgggaattag agttgatgaa
    10861 gaatctcttg atagacaatt gaaacttgct ggatgtgaag agagaaaaga gtttgactat
    10921 cataagatgc ttttaaatgg cgaacttccg tatacaattg gtggaggaat tggtcagtca
    10981 agaatatgta tgtatttctt acaaaaagct catataggag aagtacaagt tggagtatgg
    11041 ccacaggata tgattcaaaa ttgtagtagt gcaggaattg aattattata atttaaatat
    11101 aaactaattg ataaaattta tatctaatat gttcatcaaa ttaattatat cggttataat
    11161 gtagaataac ctataattat aagaggtgat aaatatggag tttttaaaaa taatcattaa
    11221 tatagtttta gatattttaa aaaaaatttt ggtcagattt aaaaatgcta aatttggttt
    11281 ggtttttgta tttgatttac ttaaactacc tgattttatg acagataaaa gaatcaatat
    11341 agttgataaa ataaaggtaa tttctgtatt aatttttact atatcctact ttgtatctgg
    11401 ggttgatata attccagaga tgattgcagg ggcatttggt tttatagatg atgctatagt
    11461 tttaatttgg agtataggta tagtgaatga agaaattaat aagtatagag ttatcgcaaa
    11521 aaaagataaa cattcaaata taattgaaaa tgtagaattt tctataaaag atgaagaaga
    11581 ataacaattt atatatgtta ttcttttttt tattaacaaa aaatggttaa attgttgacg
    11641 gagaggtaaa actctattat cataataagt ggatatccaa tatttaacta agtattcaat
    11701 gtgcaagaat gcttaattta aaaaagttat ggagggtaaa tattatgaag aaaaaaatag
    11761 gtttagtgcc caaactaatc atcggtatca tagtcggtat tttaatcgga agttatgcac
    11821 cagaaataat agtacaaata ttagtaacag ttagtacttt attctcagca ttcctaaaat
    11881 ttgttatacc atttataatt ataggttttg taacagcagg tatagctgat ttagcaacag
    11941 gtgcaggaaa gttattgggg ataactacgg gaatagcata tggttctacc ttaatagcag
    12001 gattattatc atttgcagta tctacattaa tcttcccaaa ttttattgat gcaagtgttg
    12061 caagtcaaat tggagaccca gaggcaggaa tgttagctcc aatattcaca attccattag
    12121 agcctatggt agatgtaaca gctgcaatcg tatttgcatt tgtaatggga cttggaatat
    12181 cagctttaag aaatcatgga aaaggagaga ctttatttaa tttcttccaa gaatttcaag
    12241 gaatcgtaac gaaaacgttg tcaacagtga ttataccttt attaccttta tacatagcag
    12301 gaacttttgc aaatataact atagcagggg aagtatggac aatattaggg gtattctgga
    12361 aagtatactt agtagtaata cctttacatt ttgtatatat ggcatgtcaa ttcacagcag
    12421 caggtgtatt ttcaggaaaa aatccagtaa gaatgttaaa aaatcaagta cctggatatt
    12481 taacagctat aggaactcaa tcttcagcag caactatacc agttaatgtt ggttgtgcag
    12541 aaaaaaatgg agtaagtaag caaataagag aatttgttgt acctctttgt gcaacaatac
    12601 atttatcagg tagtatgata tcaataacag gatttacaac tgcagtttta atgatgaatg
    12661 gtatgccaca tggaataggt gtattattcc cttatattgc aatgttaggt atagcaatgg
    12721 ttgcagcacc aggagcacca ggtggggcta taatgagtgc acttcctttc ctaccaatgg
    12781 ttggaatacc atcagatggt gggcttgcaa gtttaatgat agctttatac ttaactcaag
    12841 acagttttgg tacagcatgt aatgtatcag gagataatgc aatagcagta gtagtagata
    12901 aaataaatga tggaatgagt aaaaaaggaa acaaagaaga gcaaagtgct tagtttttta
    12961 ataattaaaa atgagttatc tcaaatagag atagctcatt ttttatggta taaaaaatta
    13021 taaaataaag tgattttttt atttaaaaat tgctttaaga gatgatatta ataatttatt
    13081 ttcattaggt tttaatatac atactctaaa gaaatactca tctagacctt caaatgaaca
    13141 acaatctctt attattattt tttgtgggag cagttgctct cgaagagatt tagcagtcaa
    13201 ttcctttgtc ttgattttac acaatatgaa attaccttta gtattgtata tatctaagct
    13261 ttttatattc ttaagctctt taagcaggta atctctttca gtgttcatta aggaaatagt
    13321 atttgatata aaatctaaat cactaaacat gatttccccc attttagaag ctattatgtt
    13381 tatattccat aaatctagat ttttatttat ttcatttttg acattagtgt tagatataag
    13441 gccatatcca agccttattc caggcgttga aaaaaatttt gatgtacctc ttataacgaa
    13501 taaattagaa taatcatcaa ctaattgagt acaagagtat gtatctgtat cagtaaactc
    13561 tacataagtc tcatcaatca ttaaaaatga gtctgtattt tttaaaattt ctctcacttc
    13621 taccttagta aatgcaaatc cagttggatt actaggatta cagattacta ccaaatcata
    13681 atctttagca ttgatagttt ttataagatt ttctaaattt atatgaaaat tatcttcttc
    13741 ttttgcaaag tatttttcta tagaacaatt aattttagat aactcttttt catactctga
    13801 ataggctgga gagagtaaaa gagcttgttt aggattaatt gtatgtataa atgatgaaat
    13861 tagttctgta gctccactgc caagtactat attatctata gaacatttgc agtaattgga
    13921 tatagaagtt tttaagtcta tgtaatcagg gtcaggatac atagatacca tatctatatt
    13981 gtttacaatg tattgtttgg ctaaacttga tgtaccaaaa ggatttatat ttgagctaaa
    14041 atccataaat tcttcctttg aaaagccata cttactagat aaactatata aattagctcc
    14101 atggtttgtg ctcataaaag tacctccaaa aataaaatta tataatcgat aaaaataact
    14161 gattaatata tgtacatata gattataata caaaggaatc gttattccaa atttttaatg
    14221 taaaaagcaa aaaagtatgg ataagctgtt aatttaggtt taaaaatgat gaaaaagatt
    14281 ttaattgata ttttggtatg ctataatata taagttaata gaaggaaagc gggaggatac
    14341 aaaatgtaca aagttacagg aaaaatgttg acagaagaac aaataaaaga aaaagtttat
    14401 gaattaggaa aaaagataga agaagacttt aaaggcgaag atttactagt ggtaggtata
    14461 cttaaaggag ctagtgtatt tgtttcagat ttaataagat gtatagattt ggatgtaaat
    14521 atagatttta tgagtgttac aagttatgga aactcaacag agtcttctgg aacagttaaa
    14581 atattaaaag atttagatgt agatattgaa ggaaagaatg tgttgattgt agaggatata
    14641 atagattctg gtttaacatt aagtaatcta gttgcagctt taaaaactag aaatccaaaa
    14701 tctttaaaac tatgtactct acttgacaaa cctcagagaa gaaaagctaa tatacctgta
    14761 gactatgttg gatttgttat agaagacaaa tttattgttg gttatggaat agactatgct
    14821 gaaaagtata gaaatttacc ttatataggt atagttgagg atgtagaata gatttaaatg
    14881 tgaatcaaaa gcactaattt ctaagggagt tagtgctttt tagttaatta tataaaattt
    14941 tagtaaagga gacatctata tggatattca gttaaatata ttattatttt tccaaaattt
    15001 aagaaatcca attttgaact ttatatttct tgtctttaca ataagcacag aagcaccgtt
    15061 attaatatta attacaacaa ttatatactg gtgtataaat aaaaaatgtg gacagaaaat
    15121 actgtttgct ataattggaa attttgttgt aaatttaggc atcaaagagt ttgtaaaagc
    15181 tcccagacca ataggaataa aaggtcttga atcattgaga gtttctactg ctggtggtta
    15241 ttcttttcca agtgctcata ctcaaactgc aacaacattt tgggttagta ttatgactat
    15301 atttaaaaag agatatcttc atttaatagg tgcagttatg gttttaggag taggattgtc
    15361 taggctgtat ttagctgttc attggccaat agatgtgata gcgggttgga tattaggaat
    15421 attttttaca gttgtatttg taaaaatatt tgactatatt gatgataata aaaattatat
    15481 tttacttgta gttttactta taccatttat aatagtggct atatttctaa atagccctga
    15541 atattttgaa aaatttggta taatagtagg atttgtattt ggatatatgg ttgaggatag
    15601 atttgttaaa tttaatacag ataataataa caaaaaaata aattttagta ataaaaataa
    15661 ggggagtaaa aatataattt tttcaagaat atgtaggttt ttggttggga tagtaacaat
    15721 aggaatgtta tatattggca ttaagttgtt tatttcaatg ttaatagtca ctttaaatat
    15781 gagtgactct actatgagta taatttttat gaattttatt aagaatacag tagttgtttt
    15841 ttatggaata gcaggagctc cagcattatt taaactttta aaattaaatt gattttaagg
    15901 tatagagtca atagtttaaa ttaaaaaaat taagggtaga aaagagtgat tatttgaaga
    15961 aggtagtaat aataggtggt ggaccagcag ggatgatagc cgcatcaact gcatgtgaga
    16021 aaggctatga tgtaacatta attgagaaaa atcataaatt aggaaaaaaa cttgcaataa
    16081 caggaaaagg tagatgtaat attactaacg cttgtgaaat agaggaatta atagaaaatg
    16141 tacctacaaa tggaaaattt ctttatagtg ctttttatac ttttacaaat gatgatgtaa
    16201 taagtatgtt taataatctt ggagtaaaga ctaaaacaga aagaggaaaa agggtttttc
    16261 cagagagtga taaagcattt gatattgtaa atgctcttga aagacaactt aaaagtaaga
    16321 aagtaaatat attattaaat tcgaaagtag aaaaaataat atctaaaaat aataaaattg
    16381 aaaaagtaat tttaaacgat aaaaaagaaa ttaagtgtga ttcagttgta gtagcaactg
    16441 gtggattaag ttatccactt actggttcta ctggtgatgg atataaattt gcaatttcac
    16501 aggggcacac aattatagat acaaaaccat ctttaattgg tatagaagta caagaaagtt
    16561 ttacaaaaga tttagaaaaa ttatcattga gaaatgttga aataagggtg tttaatagca
    16621 aacaaaagaa agtttatagt gattttggag aacttgaatt tactaaattt ggtttagatg
    16681 gacctataat caagtctgca agttgtagaa tgaaagatac aagaaaagaa aattacacta
    16741 tattattaga tttaaaacct gcattagatg aggaaaaact ggataaaaga gttcaaaaag
    16801 attttcagaa gtatacaaat aaaaaatttg aaaaagcatt agatgattta cttccaaaaa
    16861 aactaatacc tataataata aacttaagtg aaattaatcc aaatacggtt gttcaccaaa
    16921 tttctagaga gcagagaaaa aacttagtcc atttattaaa aaatttaaag tttacagtaa
    16981 agagatacag acctattgaa gaagctataa taacttctgg aggtgtaaaa gtaaacgaaa
    17041 taaattcaag cacaatggaa tcaaaattag tagaaggttt attttttgct ggagaagtaa
    17101 tagatataga tgcatataca ggtggattca atttgcaaat agctttttct actggatatt
    17161 tggcaggttt taattgctaa aaattacctg aattaaaaat gtttaaaatc acataatatt
    17221 atcagcaaca caaaataaat taaacagaag gaaggtaatc aacatggcaa gtaacaataa
    17281 caacaacaga acagtagtac caggagcaaa agaggcttta aaccaaatga aattagaaat
    17341 agctaatgaa cttggtatga gtaattatca acaagttgat aaaggaaacc ttactgcaag
    17401 agaaaatggt tatgtaggtg gatacatgac taaaaaatta gttgaaatgg ctgaacaaca
    17461 aatggctgga aaaagcaaca gatagttata aataattatt tataactatt ggctattagt
    17521 ttagctagag ctatctcaaa tatagaaagt aatttctaaa ttgagatagc ttttttttat
    17581 taaattatta aatagatata tttttatgta ataaatttta tgtatttgga taaaataata
    17641 caaatgaata tgttacataa ggagtgatgt aaaatgagta gtgtaaattt tgaggattta
    17701 aaaaacaaat ttattaatag tgacttagat gaaaaaataa gaatatatac aacaacagaa
    17761 ggtttatcag tagaacaatt taaagagcta ctaaaatatt atccaataca acatttatct
    17821 aaactagaaa aagcacttgg atagcagtat ttaatccaaa attaaaaagg caatttattt
    17881 aatattaaaa atatttttta ttaaataaat tgccttttta taataggttt aaattattat
    17941 gcttttgata tataattata gattctctca tttccaagtt caatagaccc agtattaata
    18001 agagtaacta ttccttctat atctctagca gttaagtatt tatatggaat taataaatta
    18061 taaccagtat tacttagtaa gttttttaat tgggttattt gagagatatt gaaacatttt
    18121 gcagttttag tagatttttt tcttaagtta gataaatttt cattttgaat atttattaaa
    18181 gtttctatat agtttctata agatatctta aagtttgtct cattgactat tttttcatta
    18241 agtatactac tgataaaatc ttgagacaat atattatctg caaatttatc tgctgttaat
    18301 tttccagatg ttatgccttt ttgatatttt ctaagaattt ttttagtata gcagtatatt
    18361 ttatttctcg tttctaaatc gagttgttct atttgcacaa aaccacttcc ttctaattat
    18421 aaaaatattt attaaaaatc tatacttaac taataaagct tcaataactt atttacaata
    18481 aatactaaat cataaaatat ttgttatttt ttattattac caagtataaa aaatattata
    18541 attgtaaaac ttacaaatat atgtaaaata tttaggagtg atgaaaatgt taaatgggat
    18601 aataaagctt ttttattctg aaattgtaaa aacaaagatt aataacttag attcaagtgg
    18661 atattcttta agagatgaat gtaaagaaat cagaaatata actctaaaga tagatagtgt
    18721 attttttaag cttttaaagt tagcttttgg ttcaatattg cttatggtag cttttaattt
    18781 aaacatggtt ttaggtatag gagtgttttt agttggtgta gtttatatac tatataaaag
    18841 aaatatggaa gaacaagtaa aagagcatat aaataatgtg aaaaataata tcgacatctc
    18901 taaaataact tttatggatg aaaaagggag aacaggtcta aatgcattaa ttacattact
    18961 tcttatagga ttattgacag gatttaactg gatattagtt gctagttttg tcgttgtttt
    19021 tgtttttact ataaagaatg tatgttaaaa tactaataaa tagtttaata tataggggga
    19081 gaaattgagt atgactaatg gcagttataa aatgaagaat ttagtggaaa tatccgctat
    19141 ggtgactgaa tctacagatt tttttaatat aaaagatgac ataatagaaa aaatgttaga
    19201 agttgttcac cctacaaagg catgtgtgaa tctattttat aaaaatgatt ctaaatatgc
    19261 ctacttagta tgttcgcaaa ccttagaata tgtacctcaa atttttccaa taaatagtct
    19321 taaaggtgca aaaattgatt ttgacacata tccagagtat atacatgagg ctgtaaatga
    19381 aaaaaagata atatatgtgg aaaatgtatt tgaagattct agagcagaag gtgaacgaga
    19441 tttagcaaaa gctgaaggtt atattgggag aatagttttt ccttttataa taaataacgt
    19501 agttgttgga tttatgactt gttttttaaa agaggatgat tatataacag aacaagatat
    19561 agattttata tcatctgttg catcactctt aagtttatca atagaaataa ctaataaaaa
    19621 taataatgta caaattttaa ttgataaact aagaggctca ataagtgcca taaatgaagc
    19681 tactaaaaaa ctatacctta ataagagtat tgatggattt ttggaaaatt taagtaaaca
    19741 agcgtgtaat attactaata gtaaagaagc tttaataatt ataaatagtc atcataataa
    19801 ggatgctata gttagttgtt ataatgttga gcaaaaaaag aaaacaaatt tatatcctat
    19861 gattgatatg tttttaaaga aagagtcatt gggggggtat ctaaataatt taaaaccatg
    19921 ctcaaaaaag ggaataaatt tggaaagtta tatatattac aaacttcagg ataaaaatga
    19981 tactatagga tgtatagttt gtgcaaatag caaaaactat actagtgatg atttaaatat
    20041 tcttagtata ttgtccaaac aagtttctgt tggtatgcaa ttatatgaac ataatgaaaa
    20101 ggaagtaaaa cacaaagttc ttgaaaacga gttgaacata ttaaataaac aacaacagct
    20161 tattatggat aaaggtaaaa tggattgtaa tgatgagaaa gaactttatt tttaccataa
    20221 accagcaagg gtagtaggtg gagattttta ttatgctaca aaagtggatg atgaacatat
    20281 agtttatatt gttgctgatg taatgggaca tggaatggta tcaaattatg ttgttgcaat
    20341 gataaaaggt gcttttaaag tattatgtaa tcaatacaat acaccaagag aaataatgac
    20401 aaatttaaat aggatgttat atgatgaatt tgacaagatg ggagtattta caacttgttt
    20461 gataggtatt atagatacta aagaaaatat gattactgtg tctaatgcag gtcactatag
    20521 tcctgtagtt gtaaagaaag atggcacaat agaaaataat ttaaattgta aaaagggcat
    20581 acctattgga ataatggaag atgctactta tgaaaataat actctttcaa tggaagatta
    20641 tgcaatggta tgtatgtaca ctgatggaat tttagagata aaaaattcat caaaagaaga
    20701 gtatggtata aaccgattag aaaactttct aaaagaaaat tttagattaa atcaacagtt
    20761 aatagtagaa aatctaaaat tggatttaaa gaacttctca tcaaaagata attatgatga
    20821 tgatatttta atagttatgt taaaggatag gtagattgat tatggattca aacgataaaa
    20881 aaggacttaa aagtccagag attataataa taaatggaag tccaagaatt aataaaaatt
    20941 gcagttttat agcaaatgaa gtaaaagcta tgctagaaga gtgtggatta agttgtaaaa
    21001 tatttaatat ctacaatatg gatattgaat attgcacagc atgtggattt tgtgaaaaaa
    21061 cagggtattg caagtttaat gatgatatga agccattata taaaatgttt gatgattcaa
    21121 aaggaactat agtaataagt cctgtacatt ttgattcaat atcagctaaa ttaaagactg
    21181 ttgttgatag aactcaagct atatatgcaa gtaaatatgt attaaataaa ccttcaattg
    21241 atagaagtaa gaaacggata ggaatgtata ttgcagtggg ggggagtaaa tcatatgatt
    21301 cacagtttaa aggtggacag attgtgatgg attttttctt taagagtata aatactaaac
    21361 ttttacacaa tatatattta aacaatacag atgatgtatc ttatatagat aatcaagaat
    21421 ttaaagatat aatatataaa aatttaaaga tatatattga ttcaatataa tttgattttg
    21481 taggtaatat tatgtataat tgtattgcaa taatttaaat aattaaaagg agggaatttc
    21541 aatgaaatta ccaatagcat tatctaataa acatgttcat ttgagtcaag ctgatataga
    21601 cgtattattt ggagcaggtc atgagttaac taaatttaaa ccattatctc aaccagggca
    21661 atatgcctgt gaagaaaaag tggatttagt aggaccaaaa ggaactttaa aaggagttag
    21721 agtactagga ccagctagag ctcatactca agttgaaata tctttagcag atggtttccc
    21781 attaggagta aaagcaccaa taaaagaatc tggtgatata gctggaactc caggtgttaa
    21841 attagtagga cctgctggag aagttgaaat gaaagaagga gttatagttg cttctagaca
    21901 tatacatatg agcaatgaag atgctgctaa atttggtgta actgataagc aaatagtaaa
    21961 agttaaaaca tctggaccaa gagcattagt atttgaaaat gtattagtaa gagctagtga
    22021 cagctttaat ctagaaatgc atgttgatat ggaagaggga aatgcagcag gcgttaaaaa
    22081 tggagattta gtagaattaa tagttgatta atattataaa tagactttat gctagtagat
    22141 attttatttg ctagcatatt tttaaatgta ataaatttct ataggataaa tttaatcgat
    22201 tagattaatt tataaaataa aggggtatta taaaaaatgt aataaaaact tacaaaaata
    22261 tgatgtaata
//
LOCUS       DAFKMX010000049        22128 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFKMX010000049 DAFKMX010000000
VERSION     DAFKMX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.049"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            444..1514
                     /locus_tag="LZB08_001519"
     CDS             444..1514
                     /locus_tag="LZB08_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315026.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="LZB08_001520"
     CDS             1498..2367
                     /locus_tag="LZB08_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBM7315027.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="LZB08_001521"
     CDS             2411..3271
                     /locus_tag="LZB08_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBM7315028.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="LZB08_001522"
     CDS             3302..4606
                     /locus_tag="LZB08_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315029.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="LZB08_001523"
     CDS             4669..5931
                     /locus_tag="LZB08_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBM7315030.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="LZB08_001524"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="LZB08_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBM7315031.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMIEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="LZB08_001525"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="LZB08_001525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBM7315032.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="LZB08_001526"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="LZB08_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBM7315033.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="LZB08_001527"
     CDS             13008..13937
                     /locus_tag="LZB08_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBM7315034.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="LZB08_001528"
     CDS             14151..15947
                     /locus_tag="LZB08_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBM7315035.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="LZB08_001529"
     CDS             complement(16087..17868)
                     /locus_tag="LZB08_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBM7315036.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="LZB08_001530"
     CDS             complement(17891..19777)
                     /locus_tag="LZB08_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBM7315037.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="LZB08_001531"
     CDS             complement(19794..20291)
                     /locus_tag="LZB08_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBM7315038.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="LZB08_001532"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="LZB08_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBM7315039.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8516 a         2966 c         3694 g         6952 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagtagcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggatg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaat
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgccg gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaacaggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatagaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaagggcaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctact
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac aaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtggatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggcttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagca
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggaaa agaatactta aaagatgaaa actcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa aacaattcag atagtagtat agaatcgttt atagaatatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagcg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaataa ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgagctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagctacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgctctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttctcct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttaaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcctcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaat
//
LOCUS       DAFKMX010000050        22107 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFKMX010000050 DAFKMX010000000
VERSION     DAFKMX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.050"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(318..509)
                     /locus_tag="LZB08_001533"
     CDS             complement(318..509)
                     /locus_tag="LZB08_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315040.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            729..1775
                     /gene="cobT"
                     /locus_tag="LZB08_001534"
     CDS             729..1775
                     /gene="cobT"
                     /locus_tag="LZB08_001534"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBM7315041.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1807..2367
                     /gene="cobU"
                     /locus_tag="LZB08_001535"
     CDS             1807..2367
                     /gene="cobU"
                     /locus_tag="LZB08_001535"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBM7315042.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2377..3150
                     /gene="cobS"
                     /locus_tag="LZB08_001536"
     CDS             2377..3150
                     /gene="cobS"
                     /locus_tag="LZB08_001536"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBM7315043.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3170..3784
                     /locus_tag="LZB08_001537"
     CDS             3170..3784
                     /locus_tag="LZB08_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBM7315044.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3851..4030
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4160..5659
                     /locus_tag="LZB08_001538"
     CDS             4160..5659
                     /locus_tag="LZB08_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBM7315045.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5698..7074
                     /locus_tag="LZB08_001539"
     CDS             5698..7074
                     /locus_tag="LZB08_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBM7315046.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYVVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTFLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7075..8046
                     /locus_tag="LZB08_001540"
     CDS             7075..8046
                     /locus_tag="LZB08_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBM7315047.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8078..9148
                     /locus_tag="LZB08_001541"
     CDS             8078..9148
                     /locus_tag="LZB08_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7315048.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9150..10037
                     /locus_tag="LZB08_001542"
     CDS             9150..10037
                     /locus_tag="LZB08_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBM7315049.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            10025..10657
                     /locus_tag="LZB08_001543"
     CDS             10025..10657
                     /locus_tag="LZB08_001543"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBM7315050.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10666..11892
                     /locus_tag="LZB08_001544"
     CDS             10666..11892
                     /locus_tag="LZB08_001544"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBM7315051.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINIFDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11889..12497
                     /locus_tag="LZB08_001545"
     CDS             11889..12497
                     /locus_tag="LZB08_001545"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBM7315052.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12487..13062
                     /locus_tag="LZB08_001546"
     CDS             12487..13062
                     /locus_tag="LZB08_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBM7315053.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13055..13807
                     /locus_tag="LZB08_001547"
     CDS             13055..13807
                     /locus_tag="LZB08_001547"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBM7315054.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13791..14912
                     /gene="cbiG"
                     /locus_tag="LZB08_001548"
     CDS             13791..14912
                     /gene="cbiG"
                     /locus_tag="LZB08_001548"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBM7315055.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSIIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFGHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14909..15634
                     /gene="cobJ"
                     /locus_tag="LZB08_001549"
     CDS             14909..15634
                     /gene="cobJ"
                     /locus_tag="LZB08_001549"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBM7315056.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15631..16383
                     /gene="cobK"
                     /locus_tag="LZB08_001550"
     CDS             15631..16383
                     /gene="cobK"
                     /locus_tag="LZB08_001550"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBM7315057.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNKFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16402..17880
                     /locus_tag="LZB08_001551"
     CDS             16402..17880
                     /locus_tag="LZB08_001551"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBM7315058.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17910..18815
                     /gene="hemC"
                     /locus_tag="LZB08_001552"
     CDS             17910..18815
                     /gene="hemC"
                     /locus_tag="LZB08_001552"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBM7315059.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18805..20304
                     /gene="cobA"
                     /locus_tag="LZB08_001553"
     CDS             18805..20304
                     /gene="cobA"
                     /locus_tag="LZB08_001553"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBM7315060.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELVPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20321..21286
                     /gene="hemB"
                     /locus_tag="LZB08_001554"
     CDS             20321..21286
                     /gene="hemB"
                     /locus_tag="LZB08_001554"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBM7315061.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21439..22017
                     /locus_tag="LZB08_001555"
     CDS             21439..22017
                     /locus_tag="LZB08_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBM7315062.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASIIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNVLVTLDLEELKKFDI"
BASE COUNT         9087 a         2115 c         4177 g         6728 t
ORIGIN      
        1 atatttgaaa ggtagatttt agtatatttt gaaaattttg ttttgaaaat ttgggattat
       61 ttaagaaatt ttgtattata aatagaagga aatagataaa ttgtatagaa taaaatatat
      121 taagataagt taataatgtt aaatgggaag tgagtttatg gaaaaaccaa atacaaaagt
      181 tgttaacttt caagagtata aagacagaaa aaaccaaaga gaagtaaata gagacgaact
      241 tttaaagctt ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac tccctatttt
      301 aatgggtttc ataataatta agacattcta agtccatcta tataatcttt gaattgtgag
      361 aaatctgatt cactacttaa ttctatggtt tctgaacctt ccgtataaaa attcttaaga
      421 tagatagtat aattattatc aacacaatca tgataaatac atctatatcc atcttcatac
      481 aaacttttta atttagaaaa atcatccaca atatcatctc cttaaagttc ttttaaatat
      541 atttttacaa aaagcatcta attgtatgca aattgacaat gtaaaataaa ggaaatgaac
      601 ataaaatgta gaataatttt tatatgtatg taaaaataaa aagaaaaagt tgaaattttt
      661 agaaaataag agtatttttt atctaaaaag agagtttaaa aaataaaaaa atgttgggag
      721 gataaaaaat gagtttatta gaaagtatat caaaaaacat atattcccta gataactctt
      781 ctattgaaaa aacaaagcag agactggata ggcttataca tccaactgga agcttaggaa
      841 agatagaaga tatttgcatg caattagcag gaatatttgg caatgaaaat tttgacacaa
      901 gtaaaaaggt tataattgct tttgcgggag accatggtgt atatgaagaa ggcgtagcac
      961 ctgaccctca aaatataaca aaattacagt ttccaaattt ttcgaaaggt ctatgcggag
     1021 ttggagtaat aagtaaattt gtaggggcag atgttgtagc tgtagatgta ggaataaact
     1081 gtgatgaaaa actggatggg gtattagact acaagataag aaaagggaca tcaaatatgg
     1141 caaaaggtcc agctatgagt aaacaagaag caattagatg tttagagata ggtattgaaa
     1201 tagcagagca gtgtatagaa aaagattata aggttattgg tattggggag atgggtatag
     1261 caaatactac tccaagtaca gcaataatat ctgtaatttc agggtgtgac ccgttagaag
     1321 ttactggtat aggtgcaggt cttaagaaag aaagactaaa gcataaggct gaagtaatta
     1381 gaaaggctat tgaaattaat aatccaaatc caactgatgg agtagatatt ttatctaaag
     1441 taggaggatt tgaaataggc tcaatggcag gtgttatatt aggttgtagt gctaatagga
     1501 ttccagtagt aattgatggg tttatttctt atgcagcagc attaattgca tataagataa
     1561 atccaaaaac tagagaatat atgatagcat ctcatttatc agcagaatca ggaactaaaa
     1621 gagcattaga catattaaaa ttagaccctc tattaaatat ggatatgaga ttaggtgagg
     1681 gaagtggagc tgctttagca tttgatataa tagaagcttc aaactatact tataaaaata
     1741 tggcaacttt tgatgaaatt gacatgggaa gataaatata aaaatttaat atgtaggagt
     1801 gttttgatga gtaaaattat tctagtaaca ggaggagcta ggtcagggaa gagtaacttt
     1861 gcagaaaaac tatgttttga tagaaataat aatacagctt atatagcaac ctctattcca
     1921 tttgatgatg aaatgaaaga tagagtaaaa aaacatcaag agaatagacc tagaaactgg
     1981 aatacttatg aaatatatga agatatatat tccataatta aggagatata taaagaacat
     2041 gaaactgtta tactagattg tgtaacatta ttagtaaata atctaatgtt tgaacacaat
     2101 ctaaatatag aagaatctag tcaagaagaa ataaacaaat tagaacaata cataaaagaa
     2161 caagttgtca aacttattga agaaatagaa aaaacaaatc tatactttgt atttgtaacg
     2221 aatgagttag gtatgggaat tgttcctggg aataaactta gtagaattta tgctgatatt
     2281 gtaggtcgta taaatcaata tatagcttcg aaaagtaatg aagtttattt tgtagttagt
     2341 ggcatagcta tgaagataaa ggagtagtat gcaattatga aaagatttat tttaatacta
     2401 cagtttttga caaggatacc tataaaatta aatgtaggat ttgatgatga attttataag
     2461 tcaatagtat attttccact tgtaggtttt gtcataggta tattatccta tttaatagga
     2521 tggatttcta tgcttttatt tgaaccattt atagcttcta taataataac tctagctggt
     2581 gtcttaatta ctggaggact tcatatagat ggattaggag atacatttga tgcaatatat
     2641 agttataggg ataaagaaaa gatgcttgaa ataatgaaag attcaagatt aggtacaaat
     2701 tcacttctag caattatgtt tgtactttta ttaaaggttg gattcgttta cgatataata
     2761 agtaataatt cattgtgggt tataattttt atgccaatga ttgcaaggtt aggagtaatg
     2821 ttattgacat ataaaactgt gacaccaaga gaaaaaggaa tgggaaattt atttatagga
     2881 aaattaacta caagtatgtt aataactgct ataatataca cattattgat agttgctctt
     2941 attactaaat ttatattttt attacctaat atagtgttga ttaaagtatt aggttcgatt
     3001 atagtggttt ttgtatttat aatattattc aaaaaacata tttataaaaa aattgacgga
     3061 gtaacaggag atatattagg ctgtggaata gaactttcag agcttgtata tttaatttac
     3121 atatatttgt taatttttat gtttttttaa agtaaaaagt ggtgataata tgataagatt
     3181 aatattaata agacatgcac tgacaaatga taataaaaaa ggaagattat caggccatat
     3241 aaatagttgt attagtgaag agggcaaact acaaataaat aaaattacca gatatctgag
     3301 taatgaaaac atagataagg tttatacaac accatctaca cgtactaaag atacagtaga
     3361 aaaaatatca aaattgaaat tattagaaat agaagaaaaa gaagctttaa gagaaattag
     3421 ttttggagac tttgaaggaa gaacttttga agaaattaaa atcaaatatc caaatgagtt
     3481 tgaaaaaatg ataaaagaag gtaacaacta tagatatcca aatggagaaa gtctgataga
     3541 ctcttataaa agagtggcga aagaaattga taatataatt ttagaaaatg atagtaattt
     3601 agatacaaag acaatactta tatgttctca tgcaggaaca ataagaaata taataacaca
     3661 tttgatttct ggtagttata agtatcactg gaactttaaa attgataatg catctattac
     3721 agttttagaa attgatggtg gatttgctgt aatagataaa atgaatttta cagacttcat
     3781 ataatttaat caaaaccaaa cattacaatc tgtgtttgta atatatctgt aaaattagca
     3841 atgaaaatag aatattagat taaaattagg ttcttaatta gatgttaaaa gggaaaaagg
     3901 ttagatgcct ttgcagcccc cgctactgtg aaaccaacga aactataaat accactgtca
     3961 gtttgatggg aaggttatag aagtaggatg aagttaagcc aggatacctg cctaatttaa
     4021 tttaaaacaa gaggtcttcg gggtgaaaga tttttaatta atatatataa aagtcctatc
     4081 tttagaagta ttaggacttt tttttatttg ttttttagta aattttaaca aggagggatg
     4141 gaaagtgggg aaaaaaataa tgctacaagg aacagcttca aatgtaggta aaagtgtctt
     4201 agtagcaggt ttgtgtagaa tatttaagca agatggttat actgttgctc cttttaagtc
     4261 acaaaatatg gctttaaatt cttttataac taaagaagga ttagaaatgg gaagagcaca
     4321 agtatttcaa gcagaggctg cgaaaataga gccaatagct gatatgaatc cagttttatt
     4381 aaaaccttct ggaaataata aaagtcaagt tattgtaaga ggtaaagttg taggagaaat
     4441 gccttcttct aagtatcatg attataaatt ggagttagta gatgtgttga aggaaacatt
     4501 taatagctta aattcaaggt atgatattgt agttatggaa ggggcaggaa gtaatgcaga
     4561 gataaactta aaagatagag atatctctaa tatgggtatg gcagaaatag ctgatgcacc
     4621 tgtaataata gtcggagata ttgatagagg aggcgtattt gcctcattag caggaacaat
     4681 gcttttatta aatgaagatg agaaaagaag agtaaaaggt gtaataatca ataaattcag
     4741 aggaaaaaaa gagcttttac aagatggtgt aaagatgcta gaagacatca taaaagtacc
     4801 tgttatagga gtagtacctt atacagatat aaaaatagaa gatgaagaca gtgtaactac
     4861 gagatttaag aaaaaaatga ataaaagtga tatacacata gaaataatta gaaccccaca
     4921 tatgtcaaat tttacagatt ttaatatatt tgagacacaa gaagatgtaa gtattagata
     4981 tgtagactat ggagaatctt taggaaatcc agatatatta ataatacctg gaactaaaag
     5041 tactatagac gatttaacat atataagaaa aagtggtctt gaatcgcaaa tcaaaaattt
     5101 acatagtcaa ggtaaattga tatttgggat atgtggtgga taccaaatgt taggtaaaaa
     5161 attaaaagac ccttatcatg ttgaaagtga aattgaagaa gttgatggaa taggtctttt
     5221 agatacagaa acaacgtttg agaaagaaaa gactacaaca caagtagaag ctactattgt
     5281 tgaatgtatc agtgaataca tgaataacct taagggaaaa aaagtaaaag gttatgaaat
     5341 tcatatggga attacaaagc gaggtaatga tgtaagaagt ttagatttaa taactaaaaa
     5401 acttgataaa gaagtgaatt atactgaggg aagtgtaaat aaagagggga atgtaattgg
     5461 gacttatctt catggaatat ttgatgaaat aagctttaca agagcaattt taaacaatat
     5521 aagaaagaaa aaaggtttag gagaattaga aagcaaagta aactctttta atgaatttaa
     5581 ggataaagag tatgataagt tggctgattt tttaagagaa catttagata tagagaaaat
     5641 atatgagatt atgaaataat gatgactttt atttataatt taggtaggag gtgtattatg
     5701 aaaaaaattt taattgcggg tactaatagt ggagtaggta aaacaacaat atcattagga
     5761 ataatgcaag ctttggttaa gagaaatatg aaagtacagc catataaagt agggccagac
     5821 tatatagacc cttcatatca cacttttata actggaagac attctagaaa tttagattct
     5881 tatatgttag atgatgaaaa aataaagtat gtagtaaata agtcctcaaa agatgcagat
     5941 atttcagtaa tagaaggtgt tatgggctta tatgatggtt ttggaataga tttagataat
     6001 tgtacaagtt cacatacttc aaaggtatta aagtcacctg ttatattagt tataaatggt
     6061 aaatctatgg cagcatctag tgctgctatg gtacttggat ataaggaact agataaagat
     6121 gtaaatataa agggtgttat agtaaataat gtaaagacta atagccatta tcaaataatt
     6181 aagagttcta tagaaaaata ttgtaatgtt gaagtattgg ggtattttcc tccaaataac
     6241 aaattctctt tggaatctcg acatctagga ttagttccta gtgtagaaat gaaatcacta
     6301 agggaaaaat tttatacctt agcagatgaa attgaacact atatagatat tgacagaata
     6361 attgaaatat cagaaagtga tgaatttgag agtgattttg attttcaaac tttgatagaa
     6421 aataataaaa taaaagaaca tgttaataat aaatcaatag ctatagcata tgataaagca
     6481 ttcaattttt attatagaga aaatatagag cttttagagg aacttggttt agaaataaac
     6541 tattttagcc ctcttgaaga tgcatcagtg ccaaatgcag attatattta tattggagga
     6601 ggttttccag aaatatttgg aaaagaatta gaagcaaatg agtcaatgag aacttccata
     6661 ttagaagcac ataacaataa tatacctatt tatgcagaat gtggaggact gatgtaccta
     6721 ggtgagaaac tattgaatca agaagaacaa gaatttaata tggttggaat atttagtgga
     6781 attagtaaga tgacatcttc tttaaagaga tttgggtact gtattggtgt agccaaggaa
     6841 aatacttttt tatctaaaaa aggggaatcc attaaagggc atgagtttca tcattcaata
     6901 tttgaaagtg atgaaaaatg tgtatattca atgagaaaag aaagagatgg aaatatagta
     6961 gaagagtggg atggtggtta tagtaaagga aacactttgg ctacatatct tcatactcat
     7021 ttttataata acttaaattg tatagaaaat tttttgaaaa aaggaaatga ataaatgagt
     7081 attttatcta tttatatagg atatgtgaca gacttaattg taggagaccc atattctttt
     7141 cctcatccag tgagatttat tggtaaactt ataaatttta ctcaaggtat aataagaaag
     7201 gtctctaaaa atgataggca gttaaaattt gggggattta tattatggtt tataaccgtt
     7261 ggaataacat atttaacaac ttatgctata gtgaagttat ttagttttaa tatattattg
     7321 agtattgtag tgaatagttt tataatttat acaacactag ccacaaaatg tttaaaagac
     7381 gaagcattaa aaatttataa tgtattaaaa actgaagata tagaaaaatc taggattcag
     7441 ctatcttata tagtaggtag ggatactact aatttaagtg aacctgaaat aataagagca
     7501 actgtagaaa ctgttgcaga aaatactgta gatggtataa ttgcgcctat gttttatgca
     7561 tttattggag gagcaccact tgctatggcc tataaagcta taaatacatt ggactcaaca
     7621 gttggatata aaaataaaaa gtataaagat attggttttg catctgctaa gattgatgat
     7681 atagccaatt atataccagc tagaatatct gtcattctta tgacaattgg aagtttcttt
     7741 ttaaattata actataggaa ctgttttaaa atatctatta gagatagaaa gaatcataaa
     7801 agtccaaatt gtgctttttc agaaggtgca gtatctggtg ctctaggaat acaactagga
     7861 ggtaccaacg tttattttgg ggaaaaagta tataagccaa caatagggga taagcttagg
     7921 gagatagata aagaagatat aattaaaact aataaaatta tgtatgcatc ttcatttgta
     7981 tctatgttgg tatttacact aatatatgtg ttatgtagga tgattttaat taacttaatt
     8041 atataaatta ttgtttgtta acttaagggg gacatagatg aaggatttag ggcatggtgc
     8101 caatgtagat gaaatggcaa gattatatgg gaaaaaccca aaagaaataa ttgattttag
     8161 ttcaaacata aatccaaatg tattaccaaa tttagagaga tacattttaa aaggattaga
     8221 agagtgtagg aattatccag atataaatta tacaaacttg agagaaaata tatcaaaata
     8281 tatagatata aatccagact ttataatacc tggcaatggt gcaacagaag tgatttattt
     8341 gcttatgaag agtattaaaa aaaaattggc tataataaat ccgacttttt cagagtatag
     8401 aagaagtgct gagttaaata atttagatat tatagattta gagttagatt tagaaaataa
     8461 ctttaaactt aatatagata taataaaaga aaatataaaa agatttgata gtttatttat
     8521 atgtaatcct aataatccga gtggtaatgt ccaagattta aaggaacttg ttcatttact
     8581 agataaacat aataaggtac taataattga tgaaactttc atggaatttg ttgaagatga
     8641 aagtaaatat agtttggtta aatatataga atcaaataaa aatatattta taattaaagc
     8701 tgtgactaag ttttttggta tgcctggttt aaggctagga tatggtctta caagcaacac
     8761 tgaaattatg aacaaaattt atgaacacaa agagccatgg actatcaatt cttttgcaga
     8821 tatactatcc aattttattt ttgaagataa agagtatatt aaaaatagta aagaatatta
     8881 catagaagaa agaaaatata tgttacaaga gttgagaaac ataagaaata taaaggtcta
     8941 tgatacagat gctaatttta tcttgataag aatatataaa aaaacaacta aagaattgaa
     9001 aaaagatttg tttaagcaag gaaacatatt agttagagat gcgtctaatt ttataggatt
     9061 agatgatagt tttataagag tggctataaa atctcatgaa gataacaaaa ttcttataga
     9121 aaatatgaaa aatttattgg gtgattaaaa tgaaatctta tggaatatgc ccagcatctt
     9181 gtggagaatt tgtacaaggt ataattgatg atgaagaata tttatgttca tatgcaatag
     9241 atatgtattc taaagtgtat attgaagaaa aattagtaga tataaattta gggagatata
     9301 agtcaaggtt agcaattgaa aaggtatttg aaaaatttaa tttacctaaa aaatatacta
     9361 aaaatatttc tttaaatata aatagtaaaa tacctgttgg aaaaggaatg gcaagttcaa
     9421 ctgctgatat aggagctact ataaaggcaa cattatcatt gatagataaa gatttaagta
     9481 gcgaggagat ttctaaatta gcagcagaaa tagaaccaac agattctata tttatagata
     9541 aaaatagtat atttaatcct ctaaatggaa ctgttataaa atacctagga aatctgacca
     9601 atgcgaaagt tgtaatactt gaaccaaaca aagtactaga tactatgaag attagattga
     9661 ggcaagatta taataaatta aaagtagaga ataaagaagt aataaaaaaa tcatttgctc
     9721 ttttagaaga aggattgaaa aaaaataatt tatctctagt tggagaggct tgcactttga
     9781 gtagcttagc aaatgagaat atagaaaaaa aagagtgttt aaatgaaata ataaaaatat
     9841 caaaaaaata tggagcatat ggagtaaata ttgctcacag tggaactgta gtaggtattt
     9901 taattgataa gtctatgaat gataaaaaga tgatagatgc cttatgtgaa tctaacataa
     9961 attctgtcta taataaaatt tatacacaaa acataattaa tggaggaatt aagggggaaa
    10021 tagaatggaa tacataaaga atcctatgaa aatagaagaa aaaagttttg aattgataca
    10081 agaaataata gacgaaatta gaccagatta taaatttaag aataatatcg aagaaaaaat
    10141 aataaaaaga gctatacata ctactgctga ttttgattac ttagatatat taaaaatatc
    10201 agaagaagca gttgatagta taatagatgc attaaaaaat aatgcaagta tttatactga
    10261 tacaaatatg gctcttagtg gtataaataa aagaaagtta gaatcattag gatgtaagta
    10321 taaatgttta gtggcagatg atgaaactgt aaaattagct aaagaaaaag gaattactcg
    10381 ttctatggca gcagtagaaa ttgcagctaa agaagaagga agaaaaatat tcgttttagg
    10441 taatgctcca acagctttat ataaagttat ggagatgaaa tcagaaggta gattagattt
    10501 agatgcagtt ataggagttc ctgtaggttt tgtaggagca caagaatcaa aagatgaagt
    10561 tgaaaaaaca gatataccat atataatttc taaaggtaga aaaggtggca gtaatttagc
    10621 agcagcaata gtaaatgcta tattatatac tatgtaggta cctatatgga agaatatgta
    10681 tatatagatg gaaaaaaata tagaagagga tatactacag gttcatgtgc aacaggtgca
    10741 tctaaggctg ctgtatatat gttgattaca aaaaatagaa tcaatactat aaatatagat
    10801 actcctaagg ggataccatt actactaaaa gttgataata taaatatttt tgatacattt
    10861 gtggaatgtt ccataaaaaa agatggtgga gatgatatag atgcaactca taccatggat
    10921 atttatgcaa gagctgaaat tgtagctaaa aatgataaga acaaagggta tcttactcta
    10981 aaggatatag atagtttaag caccaatagt gagtgtaaaa gtgagctcta taagtttatt
    11041 agggtttatg gcggaactgg tattggtgtg gttacgaaaa aagggcttag tgtggatgtt
    11101 ggtaagcctg ctataaatcc gactccactt aaaatgataa atcatgaaat aagaaagctt
    11161 ataggagata attttgaatc tatacttgga aatgataaag ttttaaaaat aacaatcttt
    11221 gctccacaag gagaaactgt agcaaaaaaa acatttaatc caagattagg tatagttgga
    11281 ggaatatcta taataggtac aactgggatt gttgaaccta tgagtgatga gggttggaag
    11341 aagtctctat ctatagaact tcagatgaaa aaagaacaag gcttagataa aataatattg
    11401 gttccaggaa atcatggaga acagtttata agagaaaaat taaacttaga tattaagtat
    11461 gtagttaggg taagtaattt tataggctat atgattaaag aagctcaaag aataggatat
    11521 aagaaaatct taatggcagg tcatataggt aaatttataa aagtatctgc tggtattttt
    11581 aatactcata gtaaggtagc agatgctaga agtgagatat tagtggcaaa cttagcttta
    11641 atgggagcta gatacgaatt tttaaataaa ataaatcaat gcgtaactac tgaagaagct
    11701 gtagagctaa taaataatag tgaatataga gaagtatata atatattaag taataaatgt
    11761 agggagagag taaagcaata cttgaatgaa gattcagatg atattgatgt ggaagtaatc
    11821 atattttcaa tggataagtc tttattaggt aaatcggata atacagatga tttagtggag
    11881 gtctttatat gataaacata ataggtctag gacctggtaa cttagactat ataacaaaaa
    11941 aaggtgaaaa tttaatttcc acttctgatg tattaattgg tggtaaaaga aatttagagt
    12001 ctataaaaaa ttttgaagga gaaaaaatag ttctggattc taatttaaga gaaattatag
    12061 agtacataaa taacaataaa gaaaagcaaa tatcaataat agcatctggg gacccactaa
    12121 tatatgggat aggtagatat ctatctaaaa atattgataa taagatgtta aacatggttt
    12181 ctgggattag ttcattacag tacatatttt caaaaatata tgtagatatg aatgatgtat
    12241 atattacaag tagtcatggt aaagtaccag attttgatta catattatca cataaaaaag
    12301 tatgtatggt tacagattca aagataggac caaaacaaat aagtagagaa ataatagata
    12361 gaaatctaaa taaaattatt gttgtaggag aaaatctgtc atatgacaat gaaaaaataa
    12421 caatagcaaa accagaagag ataataagaa tggacaattt tgatatgaat gttgtggtga
    12481 ttttagatga ggaatagtga atttataaca ggtaaagttc caattactaa ggaagaggtt
    12541 agagcaatat caataagtaa gttaaattta gtaaatgcta aaaacctcat agacataggg
    12601 gctggtacag gaagtgtaag tgttgaagct gcatataatt atcctaattt aaaggttata
    12661 tctatagaga aaaatgatga tgctatagag cttatagaga aaaataaaaa aaaattcaac
    12721 ttaaaaaata tagaagtgat aaaaggatat gctccaatta atctagggtt aaatgttaaa
    12781 gttgagtcta tttttcttgg gggaacagga aataatctgg aagaaataat agaatggtct
    12841 aaaaaaaatc ttgttacagg gggaagactt gtagctaatt ttataataat agatacattt
    12901 aatcaaactt taaaactgct taaaaagcat ggatttaaag aaatagatgt atgtgttttg
    12961 aatgtttcta aactggagaa actaggaaaa ggtgaatatt ttaaaccact aaatcctata
    13021 tatataatat cttgtgaaaa aggggaaaat gatgatgaat aaagtacatt ttgtaggagc
    13081 aggacctgga gataaagagc tgataacatt aaaaggatac aaattattaa gtaatgcaga
    13141 tgtagtgata tatgcaggtt cacttgttaa tcctgaactt ttggaatact gtaaagaaga
    13201 ttgccaaata cataatagtg cacatatgga tttacaagag ataatagatg ttatgagaga
    13261 aggtatagaa aataataaat ctgttgtaag actgcaaaca ggtgattttt ctatatatgg
    13321 ttcaattaga gaacaagtgg aagatttaaa taaattgaat atagattatg actgtactcc
    13381 aggagtaagt tcttttttag gggcagcatc atctttaggg gttgaatata cagttccaga
    13441 aatttctcaa agtgtaatta taacaagaat ggaaggcaga actccggttc cagagaaaga
    13501 atcaatacaa tcatatgcta agcatcagac atctatggta atatttttat ctgttcaaga
    13561 aattgagaaa gttgtgtcta aattactgga aggtggatat ccaaaagata ctcctatagc
    13621 agttatatac aaagcaactt gggcagatga aaaaatagtt aaaggaactt taagtgatat
    13681 agctgtaaaa gttaaagaaa acaacataaa taagactgct ttaataatgg ttgggagatt
    13741 tttaggagaa gagtataata attcaaagtt atatgataag gatttcaaac atgaatatag
    13801 agggtaatat tgctataatc tgtattacag aaaatggaaa aaaattagct ctaaagataa
    13861 attctctagt aaataactgt gttatatatc aagtaaaaaa taaaaaaagt aatttagttg
    13921 ttgaagaaac atgtgtgcat acagttcaaa agaagttaag tgattttgta ggagaaatat
    13981 ttagcaaatt tgattacata gtatttataa tggctacagg tatagttgta cgaagtatag
    14041 caccatacat aataagtaag tttagtgacc ctgcaatttt ggtaatggat gaaaaaggca
    14101 aaaacgtaat aagtttattg agtggtcata ttggaggggc aaacagcttg acacagtaca
    14161 taagtagtat tattgatgct aatccagtaa taacaacagc tactgatata aatcaaaaag
    14221 cttccttaga tatgatagca aaaaaactaa atgcacatat tgataatttt agagaaagtg
    14281 taaaagaagt aaattctatg ctagtgaata ataaatgtgt tggattatat gttgatgaag
    14341 gatatcaagt tgatactagg ggatttaaac ttatagaaag taaggatgag ttaagagata
    14401 gtttttcaaa cacatatata gaaaaattag ttgtagtaag caataaaata aattgtattg
    14461 aagatattga gaatttaaac ctagataata aatttggaca ttgctatagt accaaaaaaa
    14521 taataaaggt aattccaaga gatatagtga taggaattgg ttgtagaaga aacacagata
    14581 gtgaactgat gtataattca cttgaagagt ttcttaataa acaaaatata gatattagag
    14641 caataaacac agttggaagt atagaactga aaaaagacga aaaagcaata attgaattat
    14701 caaaaatatt gaaggtgcca tttaaaacat tttcagtaga tgaaatatct aaggttgata
    14761 atctttttga taaatctgat tttgttaaaa aaaatgtggg cgtgtattca gtagcagaac
    14821 ctgtagcata tcttttgagc aaaggtaatt tgttagttga aaaacataaa tataaaggaa
    14881 taactttttc agtggggaga ataaaaatat gatatacgtt gtaggaattg gaccaggaag
    14941 taaagataca atgactttag aagcaataaa agcaatagaa gattctgagg ctattgttgg
    15001 atataagact tatataaaac taatagaaga atttatacaa gataaagaaa taatccaaaa
    15061 tggtatgagg caagaagttg atagatgtaa acaagcagta gaggaagcaa aaaaaggaaa
    15121 aaaagtagca gttgtaagca gtggtgatgc tggaatatat gggatggcag gtcttatttt
    15181 agaattaata tctaaagaag atgaagatat aaaagtaaag gtaattccag gtgttactgc
    15241 aagtattggt gcagcagcta tacttggagc tcctattatg catgatttct gtcatataag
    15301 tttgagtgat ttaatgactc cgtgggaagt aattgaaaaa agattgagat tggccgctga
    15361 agccgatttt gtaatttgtt tgtataatcc aagaagtaag ggaagaagtg aacatctaag
    15421 caaagcattt aaaataatgg gtgagtttaa aggtggagat acacctgttg gagtggtaaa
    15481 agatgtaggt agagaaaaag aagaaaagtt tgtatgtact tttgaaaata tggactttga
    15541 aaaagtcgat atgactacaa tggttataat aggaaataag tctacctata ttaatggtga
    15601 attgatgata actccaagag gttatacagt atgattttaa ttttaggagg aacttcagat
    15661 agtttatcta tatgtgataa gataaatgaa ctgaaaaatc aaccatatat cctttcagtt
    15721 actacagatt atggaaggga acttgcacaa caacatgcag agaatgtaat tttaggtaag
    15781 cttggaaaag aagatatgct aaaatttata aaagataata atatagtaaa aataatagat
    15841 gccactcatc cttatgcagt tgaagtatcc aaaactgcta ttacttgtgc aaaattattg
    15901 aatatagatt atataaggtt tgagagaaaa tctttaattg aagaaataga ttatgataat
    15961 aaatttatag tagatacaat tgaagaagcc tgtgaaatag caaataaaat agggacaaat
    16021 atatttatag gaacaggaag taaaaatttg aacaaattta tagaggaaat aagtgataaa
    16081 aatttagttg ttagagttct tccaacgagt gaagtcatat taagttgtga aaaattaggt
    16141 ctaagtgcag ataatataat agctatgaaa ggcccattta gccagtatat aaatgaagaa
    16201 acatataaac attacaatat agatttagtc ataacaaaag aaagtggaat agctggtggg
    16261 ttcttagaga aggtaaatgc atgtaaaact ttcaatatac cagtaataat tataaaaaga
    16321 gaatcgatta actacgaaaa tgtatttaat gacataaata aaattaaaga catacttgtg
    16381 taatttttag gaggagaata tatgaagaaa gccttattag tagtgagttt tggtacaagc
    16441 tatcatgaaa cgagagaaaa gacaatagat atatgtgaag ataaaataaa aaattcgtta
    16501 aaaagccatg atttttttag agcttataca tcaaatatga taataaataa aataaaaaaa
    16561 agggatggaa tagaaataga taatccaata caggctttag ataaaatata tgaacaagga
    16621 tatgatgaag taataatcca aacacttcat attatatgtg gcgaagaatt taacaaatta
    16681 aaagaacaag tagatggata ttcttctaaa ttcaaaaaga ttgtattagg aagacctttg
    16741 cttacacata ttgaagatta tcaggaagct gtagaggcaa ttaagcatca aattcctcat
    16801 atggaaagta atgaagcagt agtatttatg ggacatggaa cacttcatga atcacattca
    16861 gcgtatccag cattggaata catgcttagg gataatggaa taaatgctta tgttggtaca
    16921 gttgaagggt atccagaaat agaacatgta ataagaagat taaaagaagg aaatataaaa
    16981 actgtaaatt tgatgccatt tatgttagtt gcaggagacc atgctataaa tgatatggca
    17041 ggagatgaag aagattcttg gaaaacaata ttagaagaaa atggatttga cgtaagagtt
    17101 catttaaaag gactaggaga aaattcttat atacaagata aatttgttcg tcatgctgta
    17161 aaatgtgcag agaatgcgaa gttctatggt ataggagcag ggccaggaga tggttcttta
    17221 cttacgataa aagcggttaa tactttaaag aaactggata ttctatacac tccagaatca
    17281 aaaaagggtg gagatagtct agctttatca atagttagtg aatatttacc tgaatcagtg
    17341 gaaataaagt ctaggcattt cccaatgagt tttgatggga atgaaaaaag tttagcttgg
    17401 gataatgtag cacaagaaat tatatcagat gttaatgatg gtaaaaatgt aggatttgtt
    17461 acattaggag accctatgat ttatagtaca tatgtatata taatgaaaag aatattagga
    17521 gaggtagaag tcgaaactat accaggaatt tcatcatttt ccaatatagc atctaatcag
    17581 aattttccat tagtcatgga caaagaagct ttagtggtaa ttccttgtac tatggaagat
    17641 gataagatag agtatgcttt gcaaaattat aattcaatag tattgatgaa agtatataaa
    17701 aattttaaag aaataataaa aaaattagaa aagtatgatt taatagaaca tgctatttta
    17761 gtaagtaatt cttcccaaga aagcgaaaat gtgtttacag atttaaaaga agcgcacctt
    17821 gaagataaga tatcttattt ttctactata ctagtaaata aagataataa aataaaatag
    17881 gtgaaagcaa tatttaaagg agacttaata tgaatatagt ggttggaact aggggtagta
    17941 atctagcgct tatacaaaca gaatgggtaa taaatgagtt aaaaaagaag tatccagaaa
    18001 ttagttttga gattaaaatc ataaagacaa aaggagattt aattcaaaat gtttcactgg
    18061 ataaaatagg tgataaagga ttgtttgtca aagaaataga gcaacaatta ttagatggaa
    18121 aaatagacat agcagtacat agtatgaaag atatgccttc ttatttagca gatggactaa
    18181 aatttgctca tacgcctaag agagaagacc caagagatgt tctaatactt agagaaggat
    18241 ataaaaattt agatgatttg cctcatggag cagtaatagg aacaggaagt aaaagaagga
    18301 agtttcaatt attgaaacaa agacctgatt taaatatagt acaagttaga ggtaatgtgg
    18361 aaactaggat acgaaaaata aaagatgaaa acatgcatgg tattgtgttg gcagcatcag
    18421 gaataattag agctaattta caagataaaa taagttctta tttacctgtt gatgttgtta
    18481 ttccagcacc tgcccaagga gctttagcta tagaaattag aagcaatgat agtacgatag
    18541 agggaatagt aaattcactg aaggatgaga atacagaaat ccaaatattg gctgaaagag
    18601 gttttttaga tggtgtaaat ggaagttgtc atattccaat ggcagcttat tgtgaaataa
    18661 tccaagataa aatacatctt actggcttat atggagattc agaaggaaaa aaagtagtaa
    18721 tcaagtctat agatggagat atttcttctc ctagagagtt aggtttaaag ttagctaaac
    18781 ttgtattgaa ggagtatgag aattatgaag ggtaaggttt acttagtagg ggcaggacca
    18841 ggtgattata aactcatgac tttaaaaggt ttagagtgta taagaaaatc ggatgtaata
    18901 gtatatgata gattagcaaa tagtagttat ttaagagaag ctaagcctga ttgtgaactt
    18961 atatatgtag gcaaggcatc aagtaatcat attttgccac aagaagatat aaatagaatt
    19021 atagcagaaa aagctaaaga aggaaaaatt gtaactcgtc ttaaaggagg agacccttat
    19081 gtatttggca gagggggaga agaagcagag actttagtag atgaaggtat agaatttgaa
    19141 gtagttccag gtataacttc tgctatagga ggtttatgtt atgcgggtat tcctataaca
    19201 catagagacc atgcttcgtc atttcatgta ataacaggtc atttgaaggg tgatgatagt
    19261 ggagaattaa actggaatgc tcttgcaaat aacaaaggta ctcttgtgtt tttgatggga
    19321 atttcaaacc tagagaaaat aagtgaaaac ttaatgaaag aaggaaaaga taaggatacc
    19381 cctgttgctc ttataagttg ggcaactaga tataatcaaa gagttgttac atctacactt
    19441 gaaaatgttt atgaaactgc tattaaagaa gaagtaaaac cgccaactct tatagtagtt
    19501 gggagtgttg taggacttag agaaaagttg aacttctttg aaagtaagcc tttatttgga
    19561 aaaagtattg ctgtaactag gtctagaaat caaaatagtg tattggtaga aaaaataatg
    19621 gatttaggtg gaaatcctat agaaatacct actattaagg tagaaaaaat acaaaataat
    19681 attaacttag aaaatgaaat aaaaaatata aataagtata attatctaat actaactagt
    19741 aaaaatgccg tagaaatctt ctttgaaaag atatatgaaa tgaattttga tttaaggata
    19801 ttatctaatt taaaggtatg tgctataggt tctgctacat caaatgagtt gaaaaaaaga
    19861 ggaattatag cggatatagt accaaaaaaa tttgtagcag agtcactgta tgaagagtta
    19921 gtgcctattt taaatgataa cgataatata cttataccta gagcagaaaa tgctagagat
    19981 tatctggttg acaaacttaa ggctatttgt gaagtaacag aagtacatat atatagaaca
    20041 gtaatagatg aggagaaaaa aggtgagata ttagatattt taaattctaa tgacatagat
    20101 tatataacat ttactagttc ctcaacagta aataatcttg ttgaaattat aggaaaagaa
    20161 aatattaaaa tattagaaaa aataaaaaca ctatctatag gtcctataac tacagagaca
    20221 ataaatagtt tgggaataaa attgtacaaa gaatctgcta catctactat agattcgtta
    20281 gtagatttaa ttataaaaaa ttaattttgg aggcttgaat atgttaagaa gaccaagaag
    20341 actaagagca agtaaagcaa tcagaaattt ggtaagagaa actaaggtga atgtagaaga
    20401 cttaatatat ccactgtttg tagtagaggg ggaaaatata aaagaagaaa ttaaatcttt
    20461 accagatgtc tatcatttat caatagacat gctagaagac gaaataaaag aaataagtga
    20521 gttgggaatt gaacatataa tgttatttgg tgttccagat gaaaaagatg agtgtgcttc
    20581 acaagctttt aatgataatg gaatagttca aaaggcaatt agaaaggtaa aagaaataaa
    20641 ttcagatata aatgtaataa cagatgtctg tatgtgccaa tatacaagtc atggacattg
    20701 tgggatttta actgatactg gttatgttga taatgataaa tcattggagt atttggctaa
    20761 aatatctgta agccatgcta aagcaggagc tgatatggtt gctccatctg atatgatgga
    20821 tggaagaata caagcaataa gagaagcttt agatgaaaat ggattagaaa atgttggaat
    20881 catggcttat agtgttaagt atgcttcaaa ttattatgga ccttttagag aggcagctca
    20941 ttcagcacca tcttttggag atagaaaaac atatcaaatg gacccagcaa atagcaatga
    21001 agctataata gagactgaac ttgatatatt ggaaggtgca gatatactta tggttaaacc
    21061 tgcattatca tatttagata ttataagaag agttaaagat aattttgact tacctctagc
    21121 agcttataat gtaagtggag aatactctat gcttaagttt gctgtgaagg aaggtttatt
    21181 aaatgaagat gtaattcttg aatcaattac atctataaaa agagcaggag cagatataat
    21241 aataacttat tttgctaaag acattgctaa actgttgaaa aaataaattc tagtagtata
    21301 aagaatatca aaaagtatag gaattgtaat taactattat acagtagttt tgtttagaca
    21361 tttattgaag aataatagaa actaatttgc aatttctata ctttatttat gtaactaaat
    21421 aaaatttgga ggtggaagat gctatatcct attaatttaa aattagatga gttagatgta
    21481 gttataattg gtggtggaga agttgcatat aggaaatgta aaaatttttt agagtttaac
    21541 aaaaatgtta ctatagtttc taaacaaatt ttaaataaat tttatgattt aaaaggaaat
    21601 ataaaaataa taaaagatga ttataaagaa atatatataa aagatgcttc tataataatt
    21661 gctgccacaa ataatagaga gttaaatatg gaaataggat tatattgtag agcaaaaaat
    21721 aagctagtga atgtagttga taatatagaa atatcgaact ttacggttcc atcatatata
    21781 aaaagaggag atttacttat aagtgtgtct actgggggca agagtccttc attatcatca
    21841 aagataaaaa aagaaattga agagagatat actgaagatt atgaggagta tttaaatgta
    21901 ttaggagata taagaaaaaa ggttataaaa aaatacgaag ataaaagtaa aagaaaagac
    21961 gtgcttaatg tgttggtaac acttgaccta gaagaactta agaagtttga tatataaaaa
    22021 ataaaataaa aaatgttaaa aaggtgttga cggattattt ttaagatgat atagtattac
    22081 ttgtccttaa gaaaagggca aacataa
//
LOCUS       DAFKMX010000051        21819 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFKMX010000051 DAFKMX010000000
VERSION     DAFKMX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.051"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..741
                     /locus_tag="LZB08_001556"
     CDS             <1..741
                     /locus_tag="LZB08_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBM7315063.1"
                     /translation="FLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQEYKLQKLD
                     SKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFKSKNSRYG
                     KVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDNAIESLST
                     IKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGYGLHLVKN
                     ILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            760..1497
                     /locus_tag="LZB08_001557"
     CDS             760..1497
                     /locus_tag="LZB08_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315064.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(1682..6739)
                     /locus_tag="LZB08_001558"
     CDS             complement(1682..6739)
                     /locus_tag="LZB08_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBM7315065.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIIQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(6764..7183)
                     /locus_tag="LZB08_001559"
     CDS             complement(6764..7183)
                     /locus_tag="LZB08_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315066.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(7416..7667)
                     /locus_tag="LZB08_001560"
     CDS             complement(7416..7667)
                     /locus_tag="LZB08_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315067.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            7756..8487
                     /locus_tag="LZB08_001561"
     CDS             7756..8487
                     /locus_tag="LZB08_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBM7315068.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            8546..9457
                     /locus_tag="LZB08_001562"
     CDS             8546..9457
                     /locus_tag="LZB08_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7315069.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFENNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            9508..10320
                     /locus_tag="LZB08_001563"
     CDS             9508..10320
                     /locus_tag="LZB08_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBM7315070.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            10429..11121
                     /locus_tag="LZB08_001564"
     CDS             10429..11121
                     /locus_tag="LZB08_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7315071.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            11208..12149
                     /locus_tag="LZB08_001565"
     CDS             11208..12149
                     /locus_tag="LZB08_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315072.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            12186..13343
                     /locus_tag="LZB08_001566"
     CDS             12186..13343
                     /locus_tag="LZB08_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBM7315073.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGKKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            13495..14055
                     /locus_tag="LZB08_001567"
     CDS             13495..14055
                     /locus_tag="LZB08_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBM7315074.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            14083..14574
                     /locus_tag="LZB08_001568"
     CDS             14083..14574
                     /locus_tag="LZB08_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315075.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            14803..15354
                     /locus_tag="LZB08_001569"
     CDS             14803..15354
                     /locus_tag="LZB08_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315076.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            15440..16372
                     /locus_tag="LZB08_001570"
     CDS             15440..16372
                     /locus_tag="LZB08_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBM7315077.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAVVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFGKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            16427..17290
                     /locus_tag="LZB08_001571"
     CDS             16427..17290
                     /locus_tag="LZB08_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBM7315078.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            17537..19399
                     /locus_tag="LZB08_001572"
     CDS             17537..19399
                     /locus_tag="LZB08_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7315079.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGKRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            19402..20868
                     /locus_tag="LZB08_001573"
     CDS             19402..20868
                     /locus_tag="LZB08_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBM7315080.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIISNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            20892..21728
                     /locus_tag="LZB08_001574"
     CDS             20892..21728
                     /locus_tag="LZB08_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7315081.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFIFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         8233 a         2465 c         3576 g         7545 t
ORIGIN      
        1 tttttacttg aaggagtatt aaatattata ctttttttgg ttttaattat tttaagtttt
       61 tatacaggct ttaaagttat aagattgata aataataata atgaacagga atataaacta
      121 caaaaactag atagtaaaat aaatcataag gataatataa tcatcaaatt aagaagtcaa
      181 aaacatgact atttgaaaca tttgcaagta gtgtattctc tattaaatac tgggctagat
      241 gaagatgcca aagaatatat aatgaatata tcaaatagct ttaaatcaaa aaattcaagg
      301 tatggaaaag tatcttattt agatgctata gcttcattaa aatacgagga atgtcttgaa
      361 aaggatatat cttttgatgt ctacattcaa gattttatgg ataatttaga gattgaacct
      421 aatgatatga gttcagtact attaaacata atagataatg caatagagtc tttgtctact
      481 ataaaaaaag aaaagaagta tataagatta catgcatatg aagatgaatt tcagtatatt
      541 atagctataa aaaataatgg tcctaaaata cataatgttg aaagtatatt ttggaatgga
      601 tttagtacta aagaaggaca agatagggga tatgggctac atttagttaa aaatatatta
      661 gataagtatg attatagtat acttgtaaat agtgatgatt ttttaactga atttgtaatc
      721 ttaatcccaa aatattatta gttaggagga gaaaagtcta tgataaaagt tttgatttgt
      781 gatgatgata agattgtaag agatgatata gcaaatatag ctaaagagtc tggatatgta
      841 gattatgtta aaaaagttaa ggatggtata gaggcaattg aatttataaa aaatgagaaa
      901 atagatttat tgattattga cattgatatg ccatataaaa atgggattga ttctgctgaa
      961 gaaattcttt ctatagataa ggatatacat atagtatttg tgacgggttt cgctgattat
     1021 agtcttgaaa gctttaaagt gcatcctata gattttgtag ttaaaccatt taaaaaagaa
     1081 aaaatacttg attcaattaa catagcaata gaccatatta actcccataa gattgctaat
     1141 aataatttta tagaagacac cctttttgta tataaaataa ggaagcagat acatatgatt
     1201 aattttgatg atatagttat gtttgagaaa aattcaagag caattaatct acacactaaa
     1261 gatgaagata taataaagtt ttatgagaac tttgatgatt taaaaaaacg cctacctcct
     1321 aatttcttta tgacccatag acaatacata gtaaatctaa gatttataca taaggttata
     1381 cctataaata aatcttcatt agagataagg tttataaatt ttcaagaaaa tgccatatta
     1441 aacaaatctc tagaaaaaga ttttttatat agattttata gggttaaaag attctagtga
     1501 agatagaagg aaaatacttt ttgtgtgaca taataagtac cgtaaagata atgtataaga
     1561 gaagtttata taaaaattat ttgtaattat gatttaatct atataaatta cagtatagta
     1621 tatattggac tttctaattt ttttaagaaa gtccaatata ttttagagat agtttttata
     1681 cttataaatt acttaagata ctagggtctt taatcttact atcttcagca agtaatagta
     1741 tcttagacat aatctcagca gttttagggt cttcatcagc gaatggaagg aatatcttac
     1801 ctctactttg agaatgtata ggaagtatat tcaaagcacc catccctttt ttatgtacaa
     1861 taccagaacc catatgcaca gaatactcac ctaaattacc tgctataaga gcatgagagc
     1921 cttcaaaatt gacattgtta atttttaata ataataaaag ttctttagca attgctatac
     1981 gcatctctac agtagaatgg ctagtttcag ggtcaacacc accagcatga gcgacactta
     2041 caacaaggtc tatatcacgc ataacttctg agaatagaat tggaggtata gaagctaatt
     2101 ctaaactttt ataagttttt ctatcaaaaa attctactgt ttcaatagta ggaggctcta
     2161 tatctgctgg tgaaaaccaa tctgcaagtg caaacatctt tgccactata ttttctttat
     2221 aaaatacttt ctgtaatccc tcttcataat cgacagtcca aagctgattt ctaagaagtg
     2281 ctacagtttt ctttggctga atttggtgac cagcatatct gcgagatata gttttttcag
     2341 ataattcatc actatttatt ctataatact cacggaatac ttgcttaaat ggttgaataa
     2401 tttgcttact aaataaatca tgttggaact cactccactt accagattca aacaaatgaa
     2461 ctggatgtgc aagttttaat ttattttttg ccgtcaactt ataagtttca ccttctatat
     2521 tgcaaataaa gaacttagac ttatcattat aaacatatcc aatattatca tcacataccc
     2581 atactagatt tttaatcaaa gggcagataa ctggattttt atagatattg gcaatttctt
     2641 taactgtaaa tactatttca tttttcatag cttgttctaa actctgtttt gcacgagaat
     2701 actgactctt taaatctttt tggacagatt taagttcaag tacatatgga tgtttagcaa
     2761 tagcttttgg tatactcttt aaagacttgt tgcctttttg aactttagta gatgcttttc
     2821 cgttttcatc aacatctatc caaattgaag taccatcaat atcttttggt ttaaatagat
     2881 gagcaacttc atttagtttt tcagtctcaa gagcccacat catacgattt acatctccaa
     2941 atccagcact tctagctaga ttttctagcg ctatagtaca agcttttgat tcactttcac
     3001 gacgttgtgc accaaattgc ttgctttctt tagcaaactt ttgaataaat tcataacgga
     3061 aagttgcttc ttttaggtct ccttttttca taggaagtaa agagaaactt ctcaatttat
     3121 cttgatttct gtttgtttca atttctttca tcaacatgtc tttatcaagc ttaccaagtg
     3181 tagcatcagc atagagttgt gcacgtctat gagagttacc accactagtt atatatttag
     3241 ctgacttgta aagtatattg aatctttctt ccccaatagt tttatatgaa tctaaaaacc
     3301 aatctacatc aaatgcacca tcaatgaaac tacgtggtga aataggggag aatagagcaa
     3361 cctctgattc tttttgtgca gagaagcctt catttatatg tgcatggaag taccaaattg
     3421 tacttttcag acccttccag tttaaatatt tttcaattat gtttacccat tgaggggcat
     3481 acatagcaga ttctatcaat cttaactcag taatgtcggt atttttaagt aaacttttta
     3541 atttttttga gtcatcttct ttagtaggat agcaattttt aagtagagaa cttaaaacat
     3601 ctttttttga ataattgcta ccatatgaat atcctctctg gaaagtttct ttaccaagag
     3661 cgaccaaaat atttacaaaa taatttatac cttcaaagta ttgtatatta ttagctagat
     3721 tagacacttc tgttggcata tcacctctac gtgattcaat ttctataatt cggtttgtaa
     3781 cagtttttaa attatcttta agccaagggt acttttctaa tgtgtcgcca gcccatctag
     3841 gatttgtcaa tgttcttatt atatttgatg cgttgtatcc acttatcatt tctctattaa
     3901 cttgctcttc agtcattaat cctaattcat gtgcacgagc aaacacattt atgctagtat
     3961 agaaaggaaa cttatattct agcaactgat aatttgaaaa cgtagtgtag aaaaacttag
     4021 taaaagaaat atcatcataa ataaacgaat gtgctttatc aagccaatag tttaaaatag
     4081 gggcatttaa agcagggcag tattcatttt gtctgtaata gtttttagta aattcaactc
     4141 tttgtttttt acattgttct atagtcaata agtttctaag tttatagtat atttctaaac
     4201 aatagttaaa tatttcttta tggtcaaact catttagtaa aactcttaaa agttttcttg
     4261 taagtccatt aaatttaagt ttttcaagtt ttgtatatat tttattttgt tttttagagt
     4321 caaggacatt tgaatatata gatgaaaacc aacttttttc tgtttcatta tatccacatg
     4381 tacttatact ataaaataag atatcaagta ttaatttgta atctaagtca agttttttac
     4441 tagtttctct ccaaagctct gctaatggat aattatcaag agtatcatta gaaactttat
     4501 atttaatagg agtcaagtta taagtagaat aacctaaaag tactttgtct acattacctg
     4561 accatctttc aacttcatat tcataatctg cattttcagt aataagctca tcaaattttt
     4621 cataaacttt tattatttta tttatatccg tatcgagaat tttttttgct tctttttttg
     4681 ttgctacaga aacagaatcc tcacgagtag ataaacttat atttgagata attttattaa
     4741 gctttgatga gctattagaa tcatctttaa aatcaaattg ttctggaatt tgaactttgt
     4801 tttcagggtt atatattcca aaaccatttt ctttgcagta atcatttttt gctttaccta
     4861 acaaattatc aattagtatt tgaacttgag atgtattctt tttagattta tcaatatctt
     4921 ctataagtaa aacaagctta ttatatagtt cttgtctagc agtattgtct tttaataagt
     4981 ttacaatttc aagacctcca aggcattttt gagaatttga agattttatc agtgaagaag
     5041 caatattttc caagtttgaa tcatcttgtt caagtaaaat ttttattact gtttgtctaa
     5101 tagagcttgt ctttaaagac aatagattgg ttatcttatc taattctaca tctgttagct
     5161 caagctttct aagtagactc aataccgttt ctctattgga aacactctta tcacttaaac
     5221 attccaataa aaattcacgt tgtctatctg tctttggttc atttacaaaa ttgactataa
     5281 gtgaagctct taaatctgaa ctcataagat gacaattttc cataagcaca tctataaatt
     5341 gagcattcat atcgtatgct gttaaagtca tcatacattt aagtatttca tgagaagaaa
     5401 tttcaatcca agtccatgga aatacacttc ctgaaaatga ttttgtcttt gaatttaaaa
     5461 tttctaaagt atgttttaaa ctttcaaatt gttcttttct agcatcaaag tctttgtcaa
     5521 taggagtgtt tgacaagtca aaattagttt ctctgttttg ccaactatag tagtagccaa
     5581 cagaaccttc tacatagaaa tttgtaatta cccatgctag tatctctaaa tcatcaccct
     5641 taatgttttt agatgctata gaatgtttaa aatatgtact ttctgtatgc gataaaaagt
     5701 aaagagctac aagtttttta tatttttcct gacaagaaag taagttttca ataagagagt
     5761 aagagtcata tatgttgtaa gttgcagtag cccacaaggc aaaataaatt tcaagattat
     5821 cattgctatt aattgattct atacggaact tttcatcagt caatgattta tatgaaagtt
     5881 ctaaacattt acgtattatt ccaggttttt ggtctgaaaa accaagtcct gtccatgtat
     5941 ccaaggctct tataacagaa ctaaatcgct ctaaattgtt gtccaaaata gtttttaaca
     6001 tgtatattga tgcagaaatt gtaccctcat ctaaactctc tacaatttgc tgtctaagtc
     6061 cttcttgttg cttggctgcc aaaagaagtt tagaaagcat ttcatagcat ttatcactat
     6121 gactcttcat gataccaatt atcatttgtc ttgtaagtag agcagtttga ttttctccaa
     6181 atattacttc ttctaaggca cttattactt tttgatttcc tttgtctatt tctagtgcta
     6241 ttttatcact cattatagag tcatagtaca agaccttatt tggaagagta agataatcta
     6301 taactgaaaa atcataataa attaaatcaa catagtttga aataaacctt ataatacctc
     6361 taaaataatc cacaagttta tttgagcgat accctttacg ataatatcct cctgaatatg
     6421 tataatccat ctttctttta attaattggc aaagttcttc tacttgttca gaagtaaata
     6481 ttaattctaa tgcatttttt aaaggtccag aaaggagctc gtccatattt ttaatttcgt
     6541 agtagtggca attgtagtat ttctttatca tatttaagaa tttgtcttca ggttctctat
     6601 aatagtatga actattttct atgcagtccc aaagagactt tgctaagtcg tagtactgtt
     6661 ttgaagaaga tttagtaaaa ttatttttaa aattttcaat tattttagga tttttttcta
     6721 ctctagtacg ttctaacatt tatgtacctc caaattattt attttaaaac attcttccag
     6781 caagcaaggt aagtcttata aaagtaaata tatctccact tttcagattt atagaggaat
     6841 cgagtttttc aataacatct tcatttataa gcactttgta gattccatct tcaaatgcct
     6901 gtaacgctac ctctattgcc ttttctacag attgtttatt ctcattgtaa atagagccaa
     6961 atccaacttt tccaatatca gcttgagatt caatttcttc attagttaag aagtttatta
     7021 ttgatttttc tgggattttc tcattatatt caagaacatt tttagataca atttcagaga
     7081 tagcttcttt taggcaattg atgttgtcag atacatcaaa aggggtaagt tctagcatag
     7141 gtttgcgttt gccaacagcc ttaactctaa aataaatttg cataaaatca cctcgcaaaa
     7201 tattaattat gtatttagaa atttaaaatt tatctatttg ttatattata tcattttgtg
     7261 aattattaat gtgaattatg gaattatttt acaaaaaata gaaaaaaagc tatcttaaag
     7321 tctaaaaaag caataaaaga taacttttaa ctataattat atagaattat aactgtacat
     7381 ttattaaatt ttaattttag aattattttt gataactatc tttttttatt tataaatttt
     7441 gctcctaaat attttttgat atcttcttca ggagaaaagt catcaataag tatttctttc
     7501 ccagtgtctt caaatactac aagaacttca cagtcacctt gtaagaaaaa ttttctaact
     7561 gtatcttctt taacttctct aacttttatt acattctttt tttctttttc aaagtctttt
     7621 gtgtaataac ttcccatcat attaattatt tttgtatatc tattcataaa atacctccat
     7681 atgcgattaa atttaaaaaa tatgttataa taattgtttg tattaactta ataattataa
     7741 caaggagtaa taataatgac tagtataaaa aataaagata ttttaaattg tatagattat
     7801 tccattaaaa ataagctctt tgacaaacta aatgatgtat ataagtcctt gccagtagga
     7861 aattgttctg gatgtggaaa ttgttgtatg gagtcagtag gtataaattt aattgagttt
     7921 ttgaatatat tttgttatct tgaagataga gttgatttaa aaaaaagctg tataagtaaa
     7981 atattagatt attattttga ggagtataca agaaagaacc catgtccatt taaagatact
     8041 aataatagat gtttaatata tgaagttaga ccattaaatt gtaggatgtt tggacattgg
     8101 aaaaaagagg attataataa aaatttagat aatgttattg agaaaaataa taactataga
     8161 gagttaatga aaaagcaata tggatttgaa ataaatgatg aagtagtaaa ttacaggata
     8221 gattattgtg atagatttat tccaaataaa gattatttaa gtaaatctaa gagattaagc
     8281 ttttttgatg agcttatgat attagactca aatatttatt caagtggaaa tatagatata
     8341 gactttaggg atagaggtat agttgaatat tttatagagt cattacttta tagaaattta
     8401 gcatataatg tgaaaataag gatatctaaa gaatacaaaa taagaaaaag aactatagat
     8461 agaataaaaa ggattgttct tgtatagttt tatattagta agtaataaat tgtatcataa
     8521 atattgtcaa aatgaggtga agaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt
     8581 tataaaaggt ggcacgaata caggaatata tgtatttgaa aataacacag cacttatgat
     8641 agacccaggg ttatgtggtt caagacctag gagagtaaat aaagtattag atgaaaataa
     8701 tatacaggtt agatatatta taaatactca tggtcatgat gaccactatg gagcttgtaa
     8761 tcaatttaaa gaatattata aagatgtgtg tataatgtca tctgaatacg ataaattata
     8821 tatagaaaat ccagaattgt tttcaaagta tattgttggg ggaaagagca atgtatttat
     8881 ggataatatc ttaaaaaata aaatgttgga tgatataaaa atagataaat ctatagaagc
     8941 tggaaaaatt gaattaaaca atgaaatgtt tgatataatt gaatttcagg gtcatacacc
     9001 tggtagtata gctgttttga cgaaggataa tgtgattttt gttggcgatt tattgatagg
     9061 agatgaaatt ttatcaaaat acgatttctt gtttctatat gatatacaag aacaaataaa
     9121 ttcattagaa aaattaaaaa atatagattt tgagtactta gttttagggc atggcaagca
     9181 agttatatcg aaagaagatt cttataaatt aatagaaaaa catttgaatg ctttaaaaaa
     9241 atatttatat caaataagag agttgcttgt agaaccgaaa tctttagaaa ttttattaaa
     9301 aaacattata aataacaata atttaagcaa taattataaa gagtatcatt tttttaaatc
     9361 ttcgttagta tctgtcataa gttacttagt tgatttagaa gaaatagggt atatattgga
     9421 aaatggagaa ttgctttact atacaaaaac aaaataaatt atgataaaat aaagaaggtc
     9481 ataaaaaaca aataaagggt gagcaatatg gaatacgaaa aatggaatga gatactagca
     9541 ccttatgaac atgcagtaga ggaattgaaa ataaaattta aaaatataag aaaagaatat
     9601 ttagcaaaag gtgaatattc tcccatagag tttgtcacag gtagaacaaa aaaaatatct
     9661 tccataatat ctaagttaaa aaggataaat gcaaaagata tagaaactga aattgatgat
     9721 attgctggta taagaataat gtgccagttt gttgaggata tatatgctat agttgatttg
     9781 ataaaagtaa gaaatgatat gactataata ggagaaaaag attatattac aaattacaaa
     9841 gatagtggtt atagaagcta tcatgttata ataaaatacc ctataaactc cattgcgggt
     9901 tcaaaggaaa ttatatgtga aatacaaata agaactcttg caatgaattt ctgggctaca
     9961 atagaacatt cattaaaata taaatatgac cattacatac ctgagactct tgctgaaagg
    10021 cttagaagag catctgatgc tgcctttctt ttagaccaag aaatgagtga aataagagag
    10081 gacataatga aagctcaggc tatgtaccaa atgaagtcta tagctattag agatactcta
    10141 aatagaatac aagagttata tgacttgggt gatacacata aagctatgca gtaccaaaga
    10201 aaacttgata gaactgaaac agataaaaat ataactgaaa ttttggaatt aaaacaagaa
    10261 atagatatat tattagagca atataaggat actaaggtag atgataaagt agttcagtaa
    10321 aaactacttg tagaaaattt gctaaatatt aatggggctg cctcttaatg gaaaataatt
    10381 tctaaaaaga gacagcctat ttagtgtaat taagaaagga atgatattat gagtttgtat
    10441 gctataggtg atttacattt ttcaacatca gtcaataagc caatgaatat ctttggaagt
    10501 aattgggatg gtcatgaaaa gaaaattata gataattgga aagaagttgt taaggaagaa
    10561 gatatggtct tagtactagg agatacatct tggggaatta atttatccga agctaaaaaa
    10621 gatttagata taataagcaa acttccaggt cagaaaatac ttataaaggg aaatcatgat
    10681 tactggtgga caaccgttac aagtttaaat aagctatatg aagacatgcg ttttattcag
    10741 acaaattttt atgaatataa agattatgca atttgtggag gtaggggctg gatatgtcct
    10801 aatgatgtta aatttgatga gactgatgaa aaagtttata aaagagaaga acatagactt
    10861 agactatcat tagaatctgc tcgtaaaagt ggtcactcaa aaataattgt gattactcat
    10921 tatcctccta cgaatgataa attggaagaa tctttattta caaagttatt tgaagaatat
    10981 aatgttgaaa aggtgattta tggtcacttg catggtaaag aatcttttaa aatgggattg
    11041 aaggggattc gaaatggagt agaatataca ttagcatctt gtgactatac agaatttaat
    11101 ttaataaaag ttcacgatta aaaaacataa attggtaaat actataagat acatgattaa
    11161 aaataaatat ttgagggatt tcggttttga gaaaggagac acgacttatg aggtggatat
    11221 tagtttttat atttttatat cttattcctt tagtagtgat attcagaaat tacaagaatt
    11281 ttaagaggtc ttgtatctac agtagtattt atgtggtatt agctacaaca ataatgataa
    11341 gtaatattta tatgagtgga cttaataaaa taaaagagtc tatgtattat cacaattata
    11401 tttctgatga gacatatgat gaaaagtatg tatcaaattt taaggaaaaa gaaagtactg
    11461 atttaaaaca ggataataaa aagaatgaag aaatatcaaa taataaaaat ttaaacaaca
    11521 aacaagatag aaatatacaa aataaagaaa gtaatgaaga aggaagtaat gatataaaag
    11581 aaaagaataa ttctgatagt aatgtttcag tgaataataa atcagaaaaa caagaagata
    11641 atacaaaaga aaataataaa tatacaagta aagaaaaaaa tagtgaagat ataaaaaaaa
    11701 tagatatgga aagtatagga atttttaaga aagaaattta taatatagaa agagcagctt
    11761 tagttccaat gagagaatgt ataccttaca ctaaggatat agcaaaaaat ataactaaat
    11821 tatctagtat aaaaaaagat gttacaaatg ccagaaacaa gtgtaaagaa gttgtaaaag
    11881 aatatgaaaa tatgcagatt ccagaacttt ctaaggagaa gtatacagaa acattgagta
    11941 atgctagaga tgacgtaaag aaagcatatg agcttagaga aaaatctatg gaaagtgctt
    12001 tgaaattaat aaatactaaa agccctaaat atatagggaa gataacagaa tatcttgatt
    12061 tatcagataa gcagatagaa agttttaaaa atagattgaa cgatttaaaa gaagaaataa
    12121 ataattcaga aacaaataat attaattaat aagatttaaa gatataagtt aaaagggaga
    12181 ttatgatgaa taaacagagt ataggaaaac taaatatgtt ttttatgggg attactcaaa
    12241 gaaaccttga taatagggat ttttttattg agttagaggt gatttttaaa tctggaaaaa
    12301 aagagtttaa aggaattgca atagaggaag ataataaata taaatttaat tttaaaggaa
    12361 aatcagattt atatacgttt aatgagttgc ttaagaatat ttctaaagag gcagaaaatt
    12421 atgatggatt agtatttaaa tatgttgagc gaggaactat tgttgtaatt gaaggagaca
    12481 ataagaaggt aaatgtaaaa tatttagata ataaggaaga agtacctaaa atagatgaat
    12541 ttacggcatc tcaaataaaa aacagagatt attatgtaaa agtaggacaa gcaaatgcac
    12601 ttttaaaaga aattggagta cttacaaaag atggtaagat aaaaaatgat aaaataagaa
    12661 aatacaatca gatagaccat tttgttgaat tgatagatag tattttaaaa gaaataaagg
    12721 ataaagactg tataactatc ttggattgtg cttgtggaaa atcttactta agctttgtct
    12781 taaattttta tataaaagaa gtattgaaga aaaactgtta ttttatagga atagattatt
    12841 cagatgtagt tatagaagca tcaaaaaata tggctaaaaa cttgggttat aaaaatatgt
    12901 catttataaa agaggatttg actaattata cacctaatag ggatgttgac cttgtaataa
    12961 gtctacatgc ttgtgataca gctacagata tggcaatagg tctaggaatt agagcaaaat
    13021 ctgaagctat agtagtagta ccatgttgtc ataaggagtt attagggcaa tatagatatg
    13081 aagctatgga accaatatta aaacatggtg tatttaaagc tagatttgca gatttgataa
    13141 ctgatggttt aagaacattg ttattggagg gtaatggata tgatacttct gtagtagaat
    13201 acatatctcc acttgataca ccaaaaaatt taatgataag agcgataaaa actaaaacga
    13261 ataatgacaa agcattaaaa gaatataaag aactgaaatc acaatttgga gtggaaccaa
    13321 ccttagaaaa acttatatat tagttaaaaa tgtaaaattt acgaaaagct tgcttaaata
    13381 tgcaagcttt ttggtattat ataggtatag tcatatccag agttttgatt tataattaaa
    13441 gctgtgctat aatatttgat taatcaaatc aataaatgtt acatggaggt aaaaatgaac
    13501 aaattgaata tatgtattga tatagatgga acaataacaa gcccttatca ttttcttcca
    13561 tacctaaatg atatctttaa taaaaatata actgaagcag agtgtataac acataattgg
    13621 gaagaattgt atggttcggg aataagagat gtgtatgcgg agtttaataa taagtatata
    13681 cactcatatg atgaagcaaa gattgtagag ggtgctacag aggtaattag cacattatct
    13741 aaaggacata atttatcttt tgtaacggct agacatgagt gtttaactga tgtaactaaa
    13801 aattggctat cgagacaagg attttcagat atagatgttt acttattagg aagtgattat
    13861 aaagttgaaa aggcaagaga actgagttgt gacatattta tagaagataa tcctttaaac
    13921 tcagtgcaac tagctgatga tggtgttaaa gtaatacttc tggatactaa ctataataaa
    13981 gatgttaaac atcaaaatat agttagagtt agtaattgga tagatattga aaagattata
    14041 aaacaaggta tttaaagatt tgtagattga aagggggagc atatgataga tttaaaaatt
    14101 gaaaatagaa aaggaaaaat ccatgtaaag agcagtgaaa taaaagacat tttagaatta
    14161 cgtccagact tcgaatatgt acaagatata tcaaatacta taaatcaaga aaatatattg
    14221 gtatttgatt gccagttgac aggaaattct tttgatatgg atgatttaga tatagaagaa
    14281 atattggaag agttaggtga agaaatagat gagtcttatt tcagtgtttt atttgaagat
    14341 gtaagagcgt atttaaaaga tgctactgat gagatagaag cagacttaca ggataattac
    14401 ttatttgata atttgagatg ttattttgat atatacaata taaatcaaga atttacagat
    14461 tttaagtttg tatttttagt ttcttttaaa gatataaaaa tttcttcatt agcaaaccta
    14521 gctaagattg tttctaagag acagcttata ggtgcatcta agttttatag ttaaaatgaa
    14581 atattattat aataaaagct agtaaccttg taaaaatata ggttactagt tttttattta
    14641 ttcattttca caaaatttca gaaaactatt tacattctaa aaaaagtaat gtatattaaa
    14701 cataatgtac attataatgg ataaaaagtt tctattaaat tactaagttt atttaataat
    14761 caaaataatt tatcttatat tagtatgtat tagggggtat ttatgcaaag tttaaatgaa
    14821 ataattgctg aaaatttaaa aaagataagg aaagaaaaac atcttagttt agacaagatt
    14881 gcacaactta gtggtgtaag taagagtatg ctatctcaaa ttgaaaagtg tgaggtaaat
    14941 cctactatat ctactttgaa gaaaataact aatggactaa aaatctcatt tacatcattg
    15001 atggagaggc aggaaagtga catagaattg attcaaaaaa gtgatataga ccattttatt
    15061 gaagataatg gaaagtatat aagttatcca atatttccat ttgactccaa aagaagattt
    15121 gagatattta tgattgaaat agaagaaggt ggaaatttag attcaaatgc tgaacttcca
    15181 ggaacacaag agtttataac tgtttttagt ggcgaagtaa ccattaaaat aaatggagaa
    15241 gattacattg tatctagtgg aaattctata agatttaaag ctgatgtatc acatatatat
    15301 agaaactcag gcaaaggtat agctaaaatg agtatggtag tatattatat ataaataatt
    15361 taatggaatt agagtgatat ttagatgatt ttaggtatga agatatatta tatttaagtt
    15421 ataggagtgt gatttatgga tgaataataa aaaagcagtt tttttctttt gtagtgattt
    15481 aaagaaagac ccagtagcca gtaatgtgat aaaatgttgt gaagaaataa tggatttaaa
    15541 tcaaactgat attgttattg atgataattt tgtattagag tttaaggata aaaacaataa
    15601 cttatttcat tttgtaaaaa caaaagatgt aataagtcat aattataaat attatcttcc
    15661 aattttaaat agacatttta gagattatga ttttgcagta gttgtaaatt ggcatgaggg
    15721 agaaaatgct ccagaacata tcctaacagc tcatacaatt ggagatgttc caacaggaga
    15781 atttggaaat tcaaatccaa gatactttaa aaatctaatc aatgctattg aggatataaa
    15841 aaatgaaaat ctacttgatg aatttactac attaacagaa gcaactcact ggtctggaac
    15901 aacatatggt gaagaatcta aattaattac agaatattct gtacctatgt tggatattga
    15961 gataggaagt agtagtgata gttttaataa tagtatagca atacaggtat tggcaaaatc
    16021 attaattaga gtatttgatt gtgatgaacc tttgaagact ttgttatgtg ttggaggggt
    16081 acattttgag aaatctttct cagatattat aaaaaataaa gaatataaca tatcaattgg
    16141 acatgtattg ccaaatcaat ggattgtaag tggaatgtat gatgatgaat ctggatttgg
    16201 gaaactagaa aagtgtataa atagtattga aggaggaatt gattgtatag tttttcatga
    16261 taaattaaaa ggtacctata aagagcaatg ccgtaaacta ggtgaaaaac ttaatgtacc
    16321 tgtatttaaa cataaaatat taaaaaatcc aaaagactta cctatatggt agttttatac
    16381 agttaatata acaataagtc ggattaattt ttaagcgagg tcaaaaatga tattatctta
    16441 ttttgcatta ttaattagag tagtgctatt agcttttgaa agagtagttg taagattatt
    16501 aggtgatgaa gaaggagata tttataaaaa tatagcttca tcatttctat ttttctttat
    16561 agggggagta tgtttacttc catttagcat aatggataga gttagtgatt ggtcgttttt
    16621 aataccatgt tatataagta gtttagtcta ttcaattggt tcagtagcgt atgtaacgtc
    16681 tttagcaact ggggaagttt cacttgttac tccaataaat agtttaaatt cattgttttt
    16741 actgctttta tcagttatat ttttaggtga aggtttaagt ttagcaaaag ttgcaggaat
    16801 cataattatg attggtggag tgtttatatt aaaaaaagta agctcttttt taaagaatgt
    16861 ctctgtaatt tttaacaatt taccatgtag attaatgttt ttatacatat ttttgcagtc
    16921 agtaggaaga gtattggata aggctttttt tgtacaagta tctcctatat tatattcaac
    16981 tatactgtat tttttcgtag gtttaaattt atttatcttt ttagcattta aaaagaaaca
    17041 aaaaataatt tgtgagattt ttactaataa aaaggggctt tcaattttaa gtggtgctat
    17101 taatggatat tcttatttag cattattaat tgctttaaat aatctagaac ttagtatagc
    17161 agagccattt tctcaagtat ctatgataat aactttaata cttgctcatt ttatattcaa
    17221 agaaaatata aaagaaaaaa taccaggttc aattttgata ctaattggtg ggtggctact
    17281 gttactttaa ttgacataaa aaatgaatta taaaggtgct attagttagt atcagaaaat
    17341 ttaaaaaagt attgacaatt cttttaggca aatgttaata taataataca aaatagtata
    17401 aatagaagac atgttactgg taatgcaggc aggatcgaat atattgcttt gatttttcaa
    17461 ggtgtttata taggttctgc cttttttgca tcttgaatgg ccattcataa aaaattatgg
    17521 agaaggagat tacaagatga attataaaaa aactgccatt gacattattg aacaagttgg
    17581 tggttcaaaa aatattttag ctttagaaca ctgttcaact agactacgct ttaagttgat
    17641 tgatagaagt aaagtcaatc aagatgcttt aaaaaaagta caaggtgtta tgagtatagt
    17701 taatggggca caatttcaag ttgtcattgg gaatagtgtt gttgatgtgt atgatgaaat
    17761 tttaaaagtt tgccctttag atacaaatga tagtactgta aaaactgaag aaaaaataaa
    17821 cttaggaaaa agatttatag aatttattat taatattttt caaccactga tttttgctat
    17881 tgcaggagct ggtattttaa aatcactgtt gatgttatta gcaatgttta atattttaaa
    17941 atcagattct actatatata cattattagt tgctatttca gatgcaacat tctatttcct
    18001 acctcttatg gtagctgtaa ctactgccaa tgtattgaaa tgtaatcgtt tagtagctat
    18061 tgccgctgta ggatacttat tgcttccagc aacaacaact gctttaagtg aaggtgtcga
    18121 actatttgga tttacaattc caaacattgc ctataatgca caggttttcc cagctatctt
    18181 atgtgtgtct ttcttggcaa caatggagaa aatttttaac aagtattcac caaaaccaat
    18241 tcgaactttc tttgttccta tgatgtcttt ggcaattact gttccaatta ctctaactat
    18301 tttaggtcct ttaggatata acgttggtac aatttttaca accattattt tattcttata
    18361 taacaaaatg ggattcttag ttgtaggatt acttgctgct tttcttccat ttatggttgc
    18421 aacaggaatg cacaaagcac taatacctta ttcaattaat acaattggaa agttgggtta
    18481 tgaagcttta tatatgacat cttcattagc ccacaatatt tctgaaagtg gtgcttgttt
    18541 tgcagtagcc ttgcgaacaa aagatgaaac attaaaacaa acagcattat ctgcaggaat
    18601 ttctgccttg atgggaatta cagagccagc tttatatggt attactttac aacataaaag
    18661 agcaatgtta ggtgttgtat tctcaagtgc aatttctggt acctttttag gattatttgc
    18721 agtaaaagga tttgtaattg ttggaccagg gcttgccagt atgagtatgt ttattgaccc
    18781 aaacaatgga aacaatttga tttttgcaag tattggtttt gttattgcaa tattaggctc
    18841 gtttattatt acactgatta tttggaagga agatacaaat acagtagagt catcaattgg
    18901 agatgttgtt gaagaagagc tagaagaaga aaaagaagta aatatcttag taactccagt
    18961 agaaggaaaa gtaatagatt taagtaaggt taatgatgaa ctatttgcaa gtaagacttt
    19021 gggagatgga gttgcaatta taccgaccaa tggaaattta tacgcacctt gtgattcaga
    19081 agttgtgatg ttatttgaaa caaaacatgc aattggttta agaacaaaaa atggggctga
    19141 gattttgatt catattggaa taaatactgt aagtatgaat ggagatggat ttaaaacatt
    19201 tgttaagaca ggcgataatg taaaagaagg agatttatta attcaatttg atttagacaa
    19261 aatctcacat gctaatttag attcaactgt tatgattgta aacaataatg gagcagatta
    19321 tgcttataaa gtgttaaacc agtcttatgg caatgtaaaa aaaggaagta tcttgtttga
    19381 tgtaaaggga ggaatataaa tatgagaaag tttccagaag gatttctttg gggaggagct
    19441 acagctgcta atcaatttga aggtggttgg aatttaggtg gtaaagggtg gtctgtatca
    19501 gatgtagcaa aggcacactt tgatgtagat gtgaaagatt ataaatctaa taatgaaatc
    19561 acaacaaaag atatagaaga aggattagca catcctgaag atgaagtaaa ttatccaaaa
    19621 cgtcatggta gtgattttta tcatcactat aaagaagata ttgctctaat ggcagaaatg
    19681 ggatttaaga catatcgaat gagtattgct tggtctcgta tttttccaaa tggagatgat
    19741 aaagagccaa atgaagaagg tttacagttt tatgatgatg tatttgatga gttgataagt
    19801 tatggaattg aaccacttgt tactatgtca cattatgaac caccattaaa tatcgtttta
    19861 aactatgatg gatggtattc acgacaagtt atcaatatgt ttgttaggta tgtagaaact
    19921 atctgtgaaa gatataaaaa caaagtgaaa tattggctta catttaatga agttgattct
    19981 atgattcgtc atccgtacac tactggtggt ttagttcgag accgttttaa ggataaaaac
    20041 tttgaggaag ttatttttca agctatgcat catcaatttg tagctagtgc cattgcaaca
    20101 aaaatttgcc atgagattat ttcaaattca aaagtagggt gtatgttaac aaaattaacg
    20161 tattatccat atacatgtag accagaagat gttttggcaa ctcaacaaaa aatgagaagt
    20221 atttatgcat atagtgatac gcaggttttt ggtgagtatc cagtatattt actgtcgtat
    20281 tttaaaaata ataacattca aatagtaaaa gaagaacatg atgatgaaat tatgaaaaag
    20341 tatccagtag attttatttc attctcttac tatatgtctt cttgtgaggc agctgataca
    20401 actggactag atattacacc tgggaatacg ttattagctg taaaaaatcc ttatttagaa
    20461 atgagtgaat ggggttggca aattgattca ataggtctac gtatatcttt gattgaatta
    20521 tatgatagat acagaaagcc tttatttata gttgaaaatg gattgggagc taaagatatt
    20581 ttaactgaag ataaaaaagt tcatgaccaa tatagaatta attatttaaa agaacacttt
    20641 aaatgtatgt tagatgcgat tattgaagat ggtgttgaac tttggggata tacttcatgg
    20701 ggttgtattg atttggtcag tgaatcaacg aaacaaatgt caaaacgtta tggatatatt
    20761 tatgtggatg ctgatgatta tggaaagggg acatataatc gatataagaa agatagtttt
    20821 tattggtata aaaaggtaat tgaaagtaat agtatagatt ttaaatgacc aatagaaggt
    20881 gagcaaataa aatgaactac ataataaaga aagttttgaa ctcgagtgtt gttttagttc
    20941 atgacacaaa ggataatgaa tttattttgc tagggaaagg aattggatat ggtaaaaaaa
    21001 caggagaata tatttgtggt tccaatgata atcaaatgtt tgtccctgtt gaaaatacaa
    21061 aatcaaaaca atttttagaa ttaatggatg atattcctgt cgacatttta aagataacac
    21121 aggaaataat agtagaggca gaaaagttat tgaactcatc ttttaataaa aacttatact
    21181 taatattagc agaccatttt aactttgcaa ttgaaaggat gcgtaaagga ataaaaatta
    21241 caaatcgagt attttgggaa ataaaaaatt attatccaaa tgagtttaag gttggaatgg
    21301 ttgcaattta tatggtagag gagagattgg gcattcaatt accagaggaa gaagcagcta
    21361 acattgcttt ccattttgca aacgctatgg cttcagatgg aaattcatat gatacgatta
    21421 agtatgccaa agtaattgga gaaataatta atatttttat tttttctttg aatagaacct
    21481 tagataaaaa gagtatgcat tatatgagat ttataacaca tataaaattt tttgtggaaa
    21541 gatttttttc agatagtatg ttaagcagtg gtgatgattt attatttaat caaatgaaaa
    21601 ggtcaaatcc aaaagaaatg ttaattgcat taaaagttag agatttttta gaaaagaaat
    21661 atggaaaaaa gttaacaaat gaggaaatag ctttcttggt tgtccatatc gcacgagtta
    21721 agcaatagag tgaattacta atattatctt atacgtctac atggtagaaa attgaaagaa
    21781 ggtaaagtat ataaagaaac tgagacatat tattttgtt
//
LOCUS       DAFKMX010000052        21583 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFKMX010000052 DAFKMX010000000
VERSION     DAFKMX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.052"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            292..1563
                     /gene="serS"
                     /locus_tag="LZB08_001575"
     CDS             292..1563
                     /gene="serS"
                     /locus_tag="LZB08_001575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBM7315082.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1932..3437
                     /locus_tag="LZB08_001576"
     CDS             1932..3437
                     /locus_tag="LZB08_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBM7315083.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3551..4249
                     /locus_tag="LZB08_001577"
     CDS             3551..4249
                     /locus_tag="LZB08_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBM7315084.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4534..5622
                     /locus_tag="LZB08_001578"
     CDS             4534..5622
                     /locus_tag="LZB08_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315085.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5861..7051
                     /locus_tag="LZB08_001579"
     CDS             5861..7051
                     /locus_tag="LZB08_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7315086.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7142..8644
                     /locus_tag="LZB08_001580"
     CDS             7142..8644
                     /locus_tag="LZB08_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBM7315087.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8716..9972
                     /locus_tag="LZB08_001581"
     CDS             8716..9972
                     /locus_tag="LZB08_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBM7315088.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10144..12918
                     /locus_tag="LZB08_001582"
     CDS             10144..12918
                     /locus_tag="LZB08_001582"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBM7315089.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12983..13168)
                     /locus_tag="LZB08_001583"
     CDS             complement(12983..13168)
                     /locus_tag="LZB08_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBM7315090.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            13543..14721
                     /locus_tag="LZB08_001584"
     CDS             13543..14721
                     /locus_tag="LZB08_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBM7315091.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14848..14933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15051..17969
                     /locus_tag="LZB08_001585"
     CDS             15051..17969
                     /locus_tag="LZB08_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBM7315092.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18155..18240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18340..19002
                     /gene="zmp1"
                     /locus_tag="LZB08_001586"
     CDS             18340..19002
                     /gene="zmp1"
                     /locus_tag="LZB08_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBM7315093.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAYADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19124..20086)
                     /locus_tag="LZB08_001587"
     CDS             complement(19124..20086)
                     /locus_tag="LZB08_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBM7315094.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20256..21446)
                     /locus_tag="LZB08_001588"
     CDS             complement(20256..21446)
                     /locus_tag="LZB08_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7315095.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8538 a         2487 c         3690 g         6868 t
ORIGIN      
        1 agttaataac tatacaagat tgataaagaa aaagtcaaaa tagataattg attagcaaaa
       61 tagatataat tataatgaag ttaaatctag caatattctc ctaaaattaa atttcattta
      121 tttaattaaa tataatttga caatgggcaa aaaagtgagt aaaatataaa taaaataaaa
      181 ataacaggtt cggataaaga aaaaggtttc ttaacctcaa gcatgaagta tattatctaa
      241 gttatttgag attaagagat aaatccaatc ttaggtactg aggaggaaaa aatgcttgat
      301 ataaaaagaa taagagaaaa tttagattgt ataaaaaaag caatggaaag aaggggcgaa
      361 aaagacttta accttgatga agtagttaag ttagatgatg aaagaagaaa gattcttcaa
      421 gaagttgaag ttatgaaaaa tgaattaaat acagcatcta aaaatatacc aaatcttata
      481 aaagaaggta aagatgtaga aaatgaaaag attaaattaa aagagttatc tgataaaatt
      541 aaggtaatag accaaaatct taaagaagta gaagataaga tggaatacct attaatgaga
      601 ataccaaatg taccacatcc agaagtacca caaggtgaaa ctgatgaaga taatgttgaa
      661 gtaagaactt ggggaaagac tactaccttt gattttgaat ctaaggctca ttgggaaatt
      721 ggaacagaat taggtatact tgattttgaa acagctagta aaataactgg ttcaagattt
      781 actctttaca aaggattagg tgctagatta gagagagcat tattaaattt ttacttagac
      841 acgaatacaa aagtaaatgg atatacagaa gttattccac catttatggc aaatagaaat
      901 agttttttag gtacaggaca gttacctaag tttgaagaag atatgtttaa aatagaaggt
      961 atggactatt ttatgattcc aacatcagaa gttcctttaa ctaatataca tgctaatgaa
     1021 atacttaaat ttgaacaatt accgataaat tatacagctt atactccatg ttttaggtca
     1081 gaagctggtt ctgctggaag agatacaaga ggtttagtaa gacagcatca gtttaacaaa
     1141 gtagaaatgg ttaaaatagt tgcaccagaa gaatcttaca atgagttaga aaagcttact
     1201 aacaatgcgg aaactatgct acaattattg aatctgcctt atagagtagt aaagatatgt
     1261 acaggtgatt taggatttac agcttctttt aaatatgatg tagaagtttg gatgccaagt
     1321 tacaataggt atgttgagat ttcatcttgt tcaaactgcg aagatttcca agctagaaga
     1381 gcaggaataa gatttaaaag agataaagat tctaaagctg aatatgtaca cacattaaat
     1441 ggctctgggt tagctattgg aagaagtgtt gcagctatat tagaaaatta tcaacaagaa
     1501 gatggttctg tggttgttcc tgaagtttta agaccatata tgggagtttc tgttattaaa
     1561 taagagttaa attatgtatt tttaagcaat acaataatat agaggtaata aaaaataatt
     1621 aatataaaag tgatacttct aatatagaag taataaaaaa aaagttaata taaaagtgat
     1681 acaataatat agaagtaata aaaaaaaagt taatataaaa gtgatacaat aatatagaag
     1741 taataaaaaa tagttaatgt aaaagtgata caataataat agaagtaata aaaaaaaata
     1801 attaatataa aaatgtgtac aaactaatga atattgatat agtaagctat catatgaaag
     1861 agtatgtaca catttttttt actaattttt cacaatatga acataaataa tttataggaa
     1921 aggaatcttt tatgaaaatt caaaatactg agtggggata tattgaatgg aaacatactt
     1981 atgatgaaaa taatccaaaa caagctatga atatatacat tgcagtaact atgcctggta
     2041 agaaacattt taatcatgtt cattatggtc aagagcagat gatatatatt ttagagggag
     2101 agggtttgta tattataaat ggagtttgga agccatttta tcaaggtatg attttttata
     2161 tagaatctgg tagcacacat gaaactataa atacaggtga tagagaaata aaagaactta
     2221 ttgtttcaaa taatgtagat gatgtaggag agagtgaagt tatagatatt aatcctaata
     2281 attatttaaa gaagaccttg ataaattata gtgaaagtac attaaatttg tatgcagcag
     2341 tagaatcaat tcgtggtcaa tttatagacc cgtttaaaat tccattaatt atatatgatg
     2401 attcatggaa tatagtttta aaaaatcctt attttccact tttttgtttt gaaaaatgta
     2461 atcctatgaa atttcctcaa aattgtgatt gtatgaatca aaaaagtagc aatcaatttg
     2521 tatgtgaata tgggataact atatataata taccaatact gtacaaaagc aatagcatag
     2581 gtgttataag aggagggtat gtactattat ctgatttgaa tttagataca gagcataata
     2641 atttatatga tattccagag ggagctgcta gaagtataaa acgcctcttg aaacaaatat
     2701 ctaaaaatat aataaatttt tgctctttta atgatataag aaaggattta caagaaaaag
     2761 agaagactat agcaagaaca tatcattatg gagagcaact tgaaatgaat ttaaaagtag
     2821 ctcaagatat ggttactaat ttaagaataa atcatcactt tttatttaat acattaaatt
     2881 caatggcaag catagcacta gatgatggta gttatgattt gtatagtgca ataatagatt
     2941 tatcaagaat gtttagatat acaatgaggt ctgatttaag atttgtagaa ttagaatctg
     3001 aaatattata cattaaaaat tatttgaatt tacagaaatt acgttatgga gatgcactta
     3061 aagtaaaata tcttattcca gagaagttat ataacctttc tgtgcctttt aactttattc
     3121 aacctattgt agagaatgca tttacacatg gatttagaga tattgacaca gaaaagcgaa
     3181 ttgaaataat tgcaagatta gattctcagt atgctattat agagattcat aataatggaa
     3241 ctatcttaga tggaaataat atagacaaaa taaaggcagg tattcgtagt aataatggtc
     3301 atggtttgtc tttaatatat acaaaattca catctgctta tggaaataat tttgatatgg
     3361 atataaagtc gagtgataat gaaggaactt acataatgat taaaattcct attgaaaatt
     3421 ataaggaata tgtatagaac ttgtgaaaaa ttaataagtt ttataaatgt taagaaatat
     3481 attagatgtt tttatgttgt tttatatatc ttactttttt atattattaa gctaaaaggg
     3541 aggtattctc atgagaagag cactaataat agatgatgaa gaagctgttc taaaaataat
     3601 aacacatttt atagaaatga aagatatgcc aataaaaatt gttggtaaag caacatctgg
     3661 tgatgaagca gtagataaaa tcattggttt agagccagac atagttttta tagatattca
     3721 aatgccaata tacaacgggt tagaagtaat agaaaaaaca tctcatttat ctaaaaaaat
     3781 aaactttgta gtaataactg catttaacta ttttgaatat gctcaaaaag ctttgagatt
     3841 aaatgtaaaa gatatactcc ttaaaccatt ggatatgaaa gaatttacta agtctgtaga
     3901 aaaaattatt ggatatcaat ataccaataa tgatttatta aatgagatac tggaatatat
     3961 aaatctaaac tatagcaatg acttaaagtt aaatgattgt gctagattat ttttaaccaa
     4021 tccatctaat atatctagga tttttaaaag taatttaaat acaactttta tatcatatct
     4081 aaatcatata agaatagaaa aatctattga attacttgaa aatactacaa aaagtataaa
     4141 tgagattgca gaaatagttg gatataatag cctaaataac ttttataaaa actttaagat
     4201 agaaaaagga atgactccaa aagtttataa actgaacact aaaaattgaa attgataaat
     4261 aaaaagtcaa tatagataat tgtttgagtg tgtctgaata agtatacttt aattatgata
     4321 aaaattaaag cgctttaaac atatttaata atattaaacc ttataaaagc tgtacctatt
     4381 tatataaaaa aatatatata aataaaaaga aaaagatata caaaataaaa aaagtgaatt
     4441 aaatactgtt tcaaaaagac agaattttca tttaattcac tgagagttta tatttcttaa
     4501 aggaaggtaa ctttataatg gaggtaattt aatatgtcag acaaagtgaa tatgaaaaat
     4561 attatctgta ttgcaggcgc ttatctagca acagcaattg gttctgggtt tgctactggt
     4621 caagaaattc tacaattttt tgcctcacaa gggtatatgg gtttaattgg agcattaata
     4681 tcaatggtat tgttttcatt tcttggtgct gaagttataa ctagaggtag agaattaaaa
     4741 cttaaagaac caacaagaat ttacactttt tattgtggga agattttagg aaaattttac
     4801 gaatggttta gtccaatatt tttatttggg attcttatag taatgatttc aggtgcaggc
     4861 tcaactttaa ctgaatacta tggtataaat ccttacttag gaagagtatt gatggctgtt
     4921 gctactttaa ttacagtgtc tatgggttta gaaaaattag taagcatact tggacgtatt
     4981 ggaccagtga tagcagtatt tacaattgct gttggattaa taagcttgtt taagcatttt
     5041 gacggtttat ctatggttgg agaagctatg aaaaatattg aagttacaaa agcatcagaa
     5101 agtccaatcg tttctggtgt aatatataat acattaaata ttattgtaat gattgttttt
     5161 ttaacaggaa tgggagcttc tattgaaagt aaaaaggatg ctttttgggg aggtttatta
     5221 ggtggaatag tgtttatgac tgctggtata gttatgtatt tggctatgat ttcagatata
     5281 ggaaatttgt atatgaaaga aataccatca ctttatttag cagataatat atctccgatt
     5341 gtaggtgtat gtttttcagt tgcattaata ttaggtatct atacaacagc tgttccactt
     5401 ttatggtctg taaccaatag aattgttgaa gatgaacatc ctaaatttaa attggtaaca
     5461 acagtaatag ctattttggc ttgtatagga ggttttttac cgtttggtaa attagtaaac
     5521 attttatatc catatacagg ttatatgggg cttttaattt tagtctgtat ggtgtataaa
     5581 gcaattacat gtagaaaaaa atcaacacaa agtcaggaat agagccactt attaatagtt
     5641 tttatttaaa aaaatttttt atataatccg aatttaaaaa gtcaaaatag ttaaataaat
     5701 tgcaaaacat gataatatat ttaattaaaa taatatagta aaatataaat ataaactaaa
     5761 attaaattta taaaaaatat ctttataaag attattaact tatataattt gtagttaaat
     5821 tataaaaaat aaaaaaataa aattttggag aggtgttagt atgcatgaac ttattttaat
     5881 ctcagaaaag tataaagaag atattttaga tttttttaca gatattcata tgcaccccga
     5941 attaagtttt aaagaattta ggactacaaa agctataaaa gatttattag taagtttaga
     6001 catagaaata ttagatttag gaatggaaac aggagttgtt ggattattaa aagggaagta
     6061 tgatggtccc acagtagcac taagaggaga tattgatgcg ttgccaatat atgaagaggt
     6121 tgatattaag tataaatcca gatatgatgg tataatgcat gcatgtgggc atgatataca
     6181 cacgtcttgc cttgtaggat gtgcatatgt attgtctcat attagagata gtcttcatgg
     6241 aaatgtaaag tttatatttc aaccagccga agaagtcaat aaaggtgcga agatgttagt
     6301 ggaacgagga gtaatggaaa atccaaaagt tgatgctata tttggacttc ataatcatcc
     6361 agatatacct tgtggaaaaa taggtgttaa gttaggtggg ttaatggcag cagtagacac
     6421 tattaagata gaagtaaatg gatttggagg tcacggaggt ataccaaaca gaacaataga
     6481 ccctatagta gcatcaagtg caattataat gggtattcaa accatagtaa gcagaaacat
     6541 aagtccatta gaatcagctg ttatatcaat aggtactata aatggaggga ctgcaaataa
     6601 tgtaatttca gaaaaggtag atatgacagg cacatgtaga agctttagta atgaagtacg
     6661 aaagaaaata tcagaaaatt tagaaaatat agtttgtgag atagctcgtg gatatcaagc
     6721 aactgcaaag ctagattatt tatttgattt accagctgtt ataaatagta aagagatgta
     6781 cgcaatagct tgtaagtctg tgtgtgattt gtattcagaa gatgctatag ttgatccaat
     6841 accttctaca ggaggggaag atttttctat attcatggaa aaagccccag gattttttta
     6901 ctggttaggg gtaggcaaca aagaacaaga ttgtatatat cagtggcata atcctaaatt
     6961 taaagctgat aaaaattcta tattggtagg tacaaatgta ctatgtcagt ctgttataaa
     7021 ttatatggat aaattaaaaa ataaaattta agatagagca ttctggagat aaagtttatg
     7081 agggggtatt agtttgagga aaattaattc acaggaaaaa aacaaagata gtggattatt
     7141 gatgagggca ctaaatgttg tagagagagc aggaaataaa cttccagacc cagtaaccat
     7201 atttttatta ctatgtataa ttgttgtaat attgtcagca gttatatcta atttaggagt
     7261 agaagaaata catccttcta caaaagaagt tgtaaaggta gtaaatctac tagagaaaga
     7321 gcagattcaa tcatatttag gtagcatagt tacaaacttt cagtcatttg ctcctttagg
     7381 attagtactg gttacaatgc ttggagcagg tgttgctgag aaatctggat ttatggaagt
     7441 gttaatgaaa aagggtataa gtaaagttcc acaaaaactt gtaactgtag caatcgtatt
     7501 tgcaggtatg ttgtcacata cagcagcaga tgtaggattt ataatattac caccacttgc
     7561 agcacttgtg tttttaggaa ttggaagaca tcctcttgta ggtatgtttg cagcttttgc
     7621 aggtgttgca gggggatttg cagcaaatgt tatgcttagt actacagatg tattgttagc
     7681 tggatttaca ataccagcag ctcaaatgat ggacccatct taccaaggca atccagcaat
     7741 gaatctttat tttgctttta tatcagcgat agttttaact tttgcaggag catttgtaac
     7801 tgaaaaattt atagctcctc gttttacaaa atataatgat tcaacaaatg ataaggatat
     7861 acaggaattg agtgaacttg aaaataaggg tataaaatac gctttattat cattgttagt
     7921 tgtagtagta gtgatagttg ccttatgtat aggtgacaat gcatttatga aagatccaga
     7981 gactggttct atactatcta gtaatgcacc tttaatgaaa ggtatagttc ctataatcac
     8041 tattatattt ttaactccag gattagtgta tggaaaagta agtaagaaga taaaaagtga
     8101 caaagattta gtatctatga tgggtagtag tatgagtgat atgggtggat atatagtcct
     8161 agcatttata gcatcccagt ttattaattt atttaatctt agtaacttag gaacaatttt
     8221 atcaataaca ggagcaaaat tactagcaga gtcgggaata cctagctatg gattgataat
     8281 aggatttata ttgctatctg gatttatcaa tttatttgta ggaagtgctt ctgcaaaatg
     8341 ggcgatttta gcacctatat ttgttcccat gtttatgcta ctagacttta atccagcatt
     8401 aactcaaata gcttatcgta ttggagatgc ttcaacaaat ccaataagtc cactttttcc
     8461 gtattttcca gttatattgg cctttgccag aagatatgat aaagatatag gtataggtac
     8521 tgtcatatcg aatatgattc catactcagt ggtatttaca ttaatagaaa taataatatt
     8581 gttactattc atgggtattg gcataccatt aggacctggt ggaggaatat cttacgtttt
     8641 atagaccgtt ataataaatt tatcaatgaa gattaaacta ataaaatata ttctttaatt
     8701 aagaggagga ttattatgtt aaaaacaaca aaaatttcag acccagagtt atacaagatt
     8761 gtagctgatg agttggttag acaagaacac aatattgaga tgatagcttc tgaaagtaca
     8821 gcaccaacag aagtacttga attaagtgga tgtgtattta caaataaaac agaagaaggc
     8881 tatccagggg ctcgttttca agcaggttct gaagaagcag ataaacttga aaccctagca
     8941 ataaaacgtg caaaagaagt atttggagca gaacatgtaa acgtacaacc gtattcaggt
     9001 tctacagcta actattgtgt atattcatca atattaaaac caaatgacac tgttttaagt
     9061 atgcgtcttg accaaggtgg acacttgaca catggtagcg cagttaattt tttacatgac
     9121 atttataaat atgaatttta tggggtagac ccaaatactg gacgtataga ttatgatgct
     9181 cttgaagcaa aggcaaaaga atgtagacca aaacttataa ttgcaggggc aagttcatat
     9241 ccaagattaa tagattatga aagaatatca aaggttgcaa aagaagtagg agcttacttt
     9301 atggtagata tggcacatgt tgcaggatta gtcgcagcga aagtaatacc tagtcctgta
     9361 ccttatgcag attttgtttc ttcatctacg acaaagactt tctgtggacc aagaagtggt
     9421 attgtacttt gtaaagcaga acatgcaaag aaactcgata aaggtgtttt tccaggaaca
     9481 ttagggtcta tacatcttaa tacagttgca gcaaaagcct tttcactatt atatttaagt
     9541 acagataaat ttaaaaagat aatggagcaa gttgtagtaa atgctcagac tctagcttct
     9601 gaactgattt cacatggttt tagtattgta agtggaggta ctgataatca tatagtaatg
     9661 gttgacttaa gaagtaagaa tcttacagga aaacaatttg aaaaagctct tgaatatgta
     9721 ggaataacag ttaataaaaa tgttatacct gacgacccac aatcaccatt tgtaactagt
     9781 ggtgttcgta taggtcttac atcaatatct caaagaggtc tgaaagaaaa agaagttata
     9841 cagatagcag ggataatgaa taaggtagca gaaaatattg ataataaaga agtattagat
     9901 gaatgtaaag cagaggcaca agaacttata tctaagtttc cattatatcc agaagggtat
     9961 tttgaagatt aaatattttg atttgtatag ctcacaattt ataaagcctg taacataaaa
    10021 tttaataata tataaccact ataaggtgtt gattaatcaa caccttattt tttttgaaaa
    10081 gtaatataca agctatcata agcatataat tccttataga taattaacaa gggggataaa
    10141 gttatgaggt attataataa acctaccaaa gaagtattaa aatacttaaa aacaaatcca
    10201 gaaattggac ttgatgataa tgaggtagaa gaaaggaagc ttagatatgg attaaatgaa
    10261 tttactataa aagaaggtag aacattttgg gacgagttgg gagaaagttt gacagaacct
    10321 atgatattaa ttctcatagg agcagcagta ataagttctt ttgtgggaga attacatgac
    10381 gcattaggta ttttaggggc tatttttatt ggaatttcta taggcataat aactgaagga
    10441 aaatctaaaa aagcagctca tgccctgtca aaattaacag aaaatataga agtaaaggtg
    10501 cttagaaatg ggaagataat taaaatatca aaaaatgatt tagtaccagg agatattgta
    10561 tatatagaaa ctggggatat gataccagca gatggaagac ttatacagtc tataaatttg
    10621 aaacttagag aagatatgct aacaggagaa tctgatgatg tagctaagaa tgctgatgct
    10681 gtattagata tggaagtagt ttattctaaa acagaaataa tcgaacagga tgcaatacct
    10741 gcaaagcaag tcaatatggt atttggaggg actcttgtag catatggtag gggcataatg
    10801 gttgtcactc atactggtga taagacagag atgggtaaga tagcacagaa tttatcaaat
    10861 gaagaccagc aaactccact tcaaattaaa ttagggaagt tgggtgctaa aatagcagga
    10921 atatcaggcg taatagctac tttattatgt atgtttatga ttatacagat gcagagaaaa
    10981 ggtatgttaa ttttagatac aagcagtgta ttatctttct tacaatcatt agaacctgct
    11041 aaaaatgcat atatggtttg tattgcatta atagttgcaa ctgttccaga aggtttgcca
    11101 actatgataa atattacctt agctataaca atgcaaaaga tggcaaagat aaatgcatta
    11161 gtaacaaaga aagaagcctg tgaaactata ggttctgttt ctgtaatatg ttccgacaaa
    11221 acagggactt taacacaaaa caaaatgatg gtagaggtag catatgttga tggtaaatat
    11281 ataagtggag gagagtatca aagtaatagc tattttgaac aaaactgtat agtaaattca
    11341 actgcagata tagaaaaaga agataattca tttaaatata taggaagcgc aacagagtgt
    11401 gcattacttt tatatcataa tgataagaac tataatgaaa tgagaaaaca aacgtatttg
    11461 atatcacaaa taccatttag ttcagaagaa aagaagatgt caacattaat tcgtcaagag
    11521 gattcagata tattactttc aaaaggtgct ccagaagtat tattaaaaaa atgttcttat
    11581 gtacaacaag gaaaaaatat tgttccaata actccaaaag tagaaaaaag tattttagat
    11641 gagattaaga agttacaaat taaatctatg agaacattag gatttgctta taaaaaaatg
    11701 agtaattcta aaacagaagt tgcaatgact tcagaaggtg aattgaattt aataggaaat
    11761 tcaagaagtt atatgaagga agataattta gtttttagtg gatttgtagg aatagtggac
    11821 ccattgagag aaggggtcaa agattccatt gataaagcat ttaatgcagg agtagatgta
    11881 aaaatgctta caggagataa tataaataca gcaacagcta ttggaaatga attaggatta
    11941 ttaaatgatg ggaaaaaggc agttgaagca acatatatag atgtattgac agataaagaa
    12001 ttgagagaag aaatcaaagg aatatctatt gttgcaagaa gtaaaccaga tacaaaaatg
    12061 agaatagttt cagcacttca aaaaagtggc gaagttgtag ctgtaacagg tgatggtata
    12121 aatgatgccc cagcacttag tcaagcagat gttggaattg caatgggtat ttcaggaaca
    12181 gaagtatcta aaaatgcagc agatataata ttgactgatg atagctttag tactatagta
    12241 gagggaataa aatggggaag aggtatatat gagaactttc aaagatttat acagtttcag
    12301 cttactgtaa atatagtagc atttataatt gctataatat ctcaacttac tggtaaggat
    12361 atgccattta ctacaataca attattatgg gtaaatataa taatggatgg tcctccagcc
    12421 ctagcattag gattagaacc tgttagagat tatgtattga aaagaaaacc aataaataga
    12481 cattctggaa taatagctag gtctatgttt gtaaatatta ttataaatgc aattcttata
    12541 attacaattg tatttactca atcagctttt aatatattgg gagctacatc agaagaacaa
    12601 ggtactgtta tattttcttt atttgcattc agtgcccttt ttaatgcatt aaattgtaga
    12661 gagtttggtt taaatagtac aataccaaat ttctttaaaa ataaactggc attgcaaata
    12721 atagtagtta cagggataat tcaaataata tttacacaag tgttccagag tttctttaat
    12781 tctgtttctt tggactttga tatgtggata aagataatac tatttgcatc cactatccta
    12841 ttgtcaaatg aatttgtaaa gctaattctt agaacaatga aaaatagtag aactatgaac
    12901 tttaatagta aaaattaatt cttatgaaac tgcaaagtac tatatattgt aactttgcgg
    12961 tttttgtttg tttaatgttg ttttatatta ccattttcat atttaatttt ttattaatct
    13021 gaacacaatc ttccattgta attgcttcat ctgtgtcaac acattcaaag tcaatatcta
    13081 atttttgtaa tattttgtat gcctcttttt gcaaaggagt atcaaattta ataggtgatt
    13141 ttgtgtaaat atcactgata aaaaacatga tttatttcat tcctttccat ttaaaatctt
    13201 atagttatag tgtatcataa ggttataaaa tatagataaa tatagtataa atttaaaata
    13261 tgtacagaca aaacaaaaaa atcatattct aagtaatact tgggaaaaat gatattatca
    13321 taataaataa tctttgtaaa aacttatata gaatctatta aacagacact aagccataaa
    13381 aactttacta aatataaaat tcgtatctaa atagatatat agaataatat tttactgaag
    13441 tagagtgatt tttaagaatt ttatatataa attatgttat cataaataaa gatatagatt
    13501 ataaaatttg ttaataaaat aaagggcagg gaggtaagtt tcatgttttc caaaatccta
    13561 gtcaaaacat ttatcagaga tagcgaaaac gttcaaaata cagatgttag aaataagtat
    13621 ggttatgtag ctggtgtagt aggtatatta tccaatttac ttttatttgt gataaaggta
    13681 ttcataggaa tgctcacatc tagtatagct ataatggctg atgcatttaa taatctttct
    13741 gatatggcat cttcagcaat aactatgata ggttttaaat tagcaagtaa gccagctgat
    13801 aaagaacacc cttttggtca tggaagaata gagtatttat cagcattaat agtagcattt
    13861 atggtgatgt tggttggact tcaatttgta aagtcatcta tagaaaggat agtaaatcca
    13921 attcctgtaa aatttgaagt aataccactt atacttttaa ttgcatctat aatgattaaa
    13981 atctggttga gtcgttttaa taaatttatg ggaaataaaa ttgattcttc agccttaaaa
    14041 gcagtttcac ttgatgcttt aggagatgta tttacttcaa gttgtgtagt tatatcattt
    14101 attgtagcta gatttactaa ttttcccata gatggatatg ttggtatcgt agtatcatta
    14161 gtgatacttt atgctggttt ctctcttgta aaagatacta taaatcctct tttaggagaa
    14221 gcaccggatg aagaaatggt aaattctatt atagagctat tgttatctta taaatatata
    14281 ataggtacac atgatttaat tattcataac tatggagttg gtagatgtat tgcatctata
    14341 catgcagaaa ttccatccaa catagatata atggaaatac atgaaattat agatactgca
    14401 gagagagaaa tatctgaaaa attagatata tatctagtaa ttcacatgga cccaatatgt
    14461 cttgaagata aagaagttat gtcagccaaa aaagaacttg aagaaatact taagaaaaat
    14521 agtcttgtaa aatctatgca tgattttaga atagttggaa aaggaagcaa gaagaattta
    14581 atatttgata tagtagttaa tccttccgaa ttttccaaaa acatgtcaga agatgactta
    14641 aaagaagata taacaaaatt agttaaggag ataaatcctg aatataattg tgtaattgtt
    14701 gtagataagg attttattta attattaatt aatctcatca taaggtaaaa aatgtaaaaa
    14761 ggatagacaa tagtatagag gcataatata atagaagtat ataattgtat ttttaatata
    14821 aataaagcaa ttttaattgt ttttatgaaa tattatagag atgttgaagt atattctatt
    14881 attgggcacc ttatggatat actgagtcag tggtgcaacc ggctatgaat ataaatttat
    14941 ttattttcat agcttttttt gtataatttt ttagtaaaaa ttactttata tacgatatta
    15001 atggaagatt agattataaa ttataggtaa cctaaagggg tgagatttac atgaagaaag
    15061 gaaatagaaa ggcattatta attagtttaa ttatgatttt aagtatggtg gtgtctacaa
    15121 tatatccaac tgtatcttat gcttcagaat taggagagaa tagtcagatt caaagtggtt
    15181 caactaattc atcaactgga gaggagaagg aaagcgacaa taaaaagcca gaacagactc
    15241 cagagaaaga taaggcaaca gataataaaa aaccagaaca gaccccagag gaagaaaaac
    15301 caacagataa taaaaaacca gaacagaccc cagaagaaga taagtcaaca gataataaaa
    15361 agccagagca aactccagaa ggagaaaagc caatagataa taaaaagcca gagcagatac
    15421 cagaagaaga taagtcaaca gataataaaa agtcagaaca agctttagaa gatgaaaaac
    15481 cactagacaa taaaaataca gaaaaaactc cagaggaaga taatctatta gaagatgaaa
    15541 atttattgaa agttttagaa gaagaactta atgaagaaaa tgaagactat ggatttgttg
    15601 taaaaattaa taacaataca atagaaacgg aatcaatgaa aaaaatatca tttaatttaa
    15661 cttatactcc aacatcaaaa ggaatacaag caggagattc aataactttt aaggtgccag
    15721 atgtattcaa taaagttaat ttagattata caagtgaatg ctttgataaa acagaaagta
    15781 atggagagta tacacttaca tttagagaat tacctaatgg ccaaagtgta atgcaaggta
    15841 aaataggttt ggaagcatat gttaaaaagg ttgatgaaga tactaatgca aaaatacata
    15901 ttgaaactac tggaaaaata gaaagtggaa gtggagatat agatgtagaa ataaaacctg
    15961 gagataaaac ggatgttcca gatgctaaag gaacacttaa aaagcttgtt gaaggaaaaa
    16021 aatcaactac tgtatttatg ccagttaaaa ataaagatat aaattacagt atacaagtaa
    16081 atgaaaagca ggaagagtta aaagatatta ttttgtatga tgagcttcct gaaggattga
    16141 cattgataaa tggttctgta agtgtagtaa caagtgatgg aaaagaagtt agtgatttta
    16201 atattgaaca gagtaaaaat tctatttcag ttaattttgg aaatatagat aaatcgtata
    16261 ctgttaagta taaggctaga atcagtgaca aaaatgcaaa acatggtaat aaatataaga
    16321 atgtagctag gattgaatct gatggtaaga agatacaaga agatgatgct actgtaagta
    16381 tatttgatag aggagatgat tatcttctta ctaaaggaca ttcaggagca acaaatatta
    16441 ctcaagttgg tcaagtaata aactatcaga tttcaataaa tgatgataaa tcaccaataa
    16501 gtaatgttgt tataacagat aatataccag aaggcatgag attaactact tcaggagaag
    16561 ctgggcatga ttttagagtt gtagaaattc caatgaatgg gagctggact ccttggagta
    16621 aagaaaaaat tgccaataat atttcatata aagtagaaga aaaaagaaat gaaagtgggc
    16681 aagtagataa agttataact ggatttacaa ttaatctcag taaagaagaa gtggaatcaa
    16741 agttttttat agcttataca ttaaaggtaa ttagtattga agatagctat ataaatagag
    16801 ctgttcttga tgcaaataat agtgaaattg ataaaaatga tgagataaac tttaagaaaa
    16861 attctggatt aataagcgca aaaaaagaag ttgataaaaa agttttaaac agtagtgata
    16921 accagattgt taaatacaaa atcaatatgt caacttatgg agtttatgat gcaggacaag
    16981 taaatctttt agatgaagta aactcagttc tagaaatatc taatataaaa tattctgata
    17041 atcttgaact taaaaaagaa gctggagatg gaaaaaatac aataagatta gtaaataaat
    17101 acgaatttaa acagataaaa gaaggggaac ctgtgcaaag ttgggttaca tttgatgcaa
    17161 acttcacaaa tgtaaaagtt ggggagacta tacgaaatgt ggctcagatt aatggttcaa
    17221 gtcctccagg agtagagact actaagcaag gttatgcttt tgaagctaaa aaagtggatg
    17281 ctttagataa aaatgtactt tcaggtgcta agttcaattt agaagatgca tttggaaata
    17341 tagtagttaa ggatttagtt agtgatgaag atggtataat tcaatctagt gtaaaaaacc
    17401 caggaacata ttatttagtg gagataatgg ctccacgtgg atatgaaaaa ctgaaggata
    17461 aagttaaggt tgaaattggt aatgaagata ttggtaagat agtagatatt gggaatatag
    17521 aaaatctaaa acaagaaaat ccaccagtaa atccaccaat acctccagat acagatgaac
    17581 caatagtaaa tccaccagta ccaccaagca cagataaacc aaggaaacca tcaagttcat
    17641 ctgatacaga gaatactata gttataaatc caccagtacc accatcagaa gatataataa
    17701 atccgccaat accagaggta ttgaatccgc cagtaccacc atcagaagag atgatagaaa
    17761 ctcctgttaa acagattatt ccaataccag aagtagtaaa gccatcagtg tcagaagaaa
    17821 aaaataataa agttaaagat gatactttgg taaatccacc agtaccacca aaaacagggg
    17881 atagtactac aattattgga gaaatacttt tggttatagg agctattgta ggacttatcg
    17941 tattaagaag aaataaaaat acaaattaga ccctaacatt ataaaaaata taagttatat
    18001 taaaaaatat cttctaaaac aatatttaat aatgaaaata ttgttagaag atattttttt
    18061 ttgaagattt atgtaaaaaa ataataactt tttagaaaaa agttccaaag ggatatagac
    18121 aattaaaaag atataagcta taatataaat ataaaattaa atgaggcaaa actagggaaa
    18181 cctagtcacg caaagctata ggggctaaag tttagtaaac catgccagcc agttgccgaa
    18241 aagagtaaaa atttcttttt gtatatacaa agatactctt gggtatcttt tttattttga
    18301 aatttattac tcccggccta actcaaagga gggaataata tgagaccaag taaaaaatta
    18361 ttaatagcta taatatcaat attcttaata agttcagttc cagtaagtgc atatgcagat
    18421 agtactacta tacaacaaaa taaagacaca cttagtcaaa tagtagtttt tccaactgga
    18481 aattacgata aaaacgaagc taatgctatg gtaaatagat tagctaatat agatggaaag
    18541 tatttaaatg cattaaaaca aaataactta aaaataaaat tattaagtgg aaagttaaca
    18601 gatgaaaaag agtatgctta tttaaaaggt gtagttccta agggatggga aggtactgga
    18661 aaaacttggg atgatgttcc aggtttaggt ggaagtacag tagctcttag aataggattt
    18721 agtaacaaag gtaaagggca tgattcaata aatttagaat tacatgaaac agcacatgca
    18781 atagaccata tagtattaaa tgatatatct aaatcagcac aattcaaaca aatatttgct
    18841 aaagaaggtc gttctttagg taatgtaaac tacttaggtg tatatccaga agaatttttt
    18901 gcagaatcat ttgcatatta ctatttaaat caagatacaa atagtaagtt aaaatcagct
    18961 tgtccacaaa cttatagctt tttgcaaaat ttagctaaat agagttttaa taaatttaaa
    19021 ataaatataa ataaagagat gctattataa tagcatctct tttatattgc gtttaaatct
    19081 aataaatttt gtcttatgta taactatgat agattatcaa ttactactcc ttatacaagg
    19141 cttcaacgtg tttattatgc atttgagtaa ttatcgtagg tataatagaa attattgaac
    19201 caattactat caataaaatt cctataacca tgtttatagg gataatttct ttaagtatta
    19261 ataatgctag tattggagat attactggtt taaagaagaa tactaaagat gtaagactag
    19321 tagatgtagc ttccattgcc ataaagtaaa gtgcataacc aacacctgta acaaatacga
    19381 atacataaat cataatgggc atattgtgta aggtatatcc actaaaaata gggatatttg
    19441 caaatacatg aagcttattt ttatttaata tatctgcgat aaaattaatt ttagtgagta
    19501 aaaccaatat caacatttcc aaactaccaa atataaagct aaagcaggtt acaactattc
    19561 caccaaattt taaggttttt cttttaccaa atacagtata cattgaaaaa ataatagcag
    19621 aagataatgt taagaatatt ccactaagag ttagttttgt atgcaatgga tttattataa
    19681 ctataattcc aagaacttct aatataagtg aaattacatt gtgcctatat attttttcct
    19741 tcaaaattaa gtaagctaaa agcattacaa agactggatt acaactgaac aaaacagcaa
    19801 ctacagatgc ctttgtgttt agaacagcaa gttgatacaa tgacatactt actactacac
    19861 acataaaacc taaaaatgca aagtatttta agtctgattt tgtaatagaa agactaagag
    19921 ctcttaatct ttttacagca aaaggtagta agactaaacc gccaacaaaa aatcttgata
    19981 aagttatttg taagggatta aagtcattag tgacagtttt tagactaatt tccattgaac
    20041 taaaaaataa ggtagtcagt attatataaa cataaccttt tttcaagtca aacacctcct
    20101 tgtaatttat attagtacta tagataagaa tattcaagca tattggaata atatggtatg
    20161 tataaaatat agtacataaa attataaact accttaatat agaatgtaag tattctatac
    20221 taaggtagtt aattatttga attactttga tttagttatt ttaaattata taatttggct
    20281 aatttttcaa atgctggtaa agaactttta actttatcat aagatacaca atatgatact
    20341 ctaaaatgac ctggacaacc aaaagaagaa cctggtacta ataataaatt aaattgtttt
    20401 gcatcattgc agaatttttt atcatcttca attggagctt ttgggaacaa atagaatgta
    20461 ccattaggtt tgacacaaga atatcccata tcgattaaac tattatatag aaggtcaaaa
    20521 ttctttttat aaatatttac atcaacttct gcatctaaac ttcttgcaat aaccctttga
    20581 aaaagagaag gagcatttac aaatccaagt attctatttg caacatttaa tgaagacata
    20641 acatcatcaa aatcatccat ttgcccatta gcaacaatat atcctatacg ttcaccaggt
    20701 agagataatg atttactata agagtatcct ataaatgaat ttctatagta ttttagtaca
    20761 caaggaactt tggcaccatc ataaattatt tctctatatg gctcatcaga aattaggtat
    20821 atgcttgtat taaattcttt ttgttttgaa tccaataatt ctccaagatt ttttaataat
    20881 tcttcagaat atatgacacc agtaggatta tttggagtgt ttattattaa agctcttgtt
    20941 tttggagtaa tagcattttt tagagcatct aaatctggct caaaagtctc tatatttgta
    21001 ggaacttcta ttaattttcc atcatagttt tctgtataat ttttatactc accaaaatac
    21061 ggtgcaaaag caataacttc atcaccagga tttaaaagag tttttaaaat aatatttaga
    21121 ccaccagcag caccacaagt cataattaaa ttttctcttg taagatttaa tccatctttt
    21181 ttatttatgt gttcagcaat agcatctctt acatcctcat atcctgagtt gttcatatat
    21241 ccatgaacta aatttggaga ttcttcattt aaaatatcat ttataactgc tttaatagtc
    21301 tctggaggtt caacacttgg atttcctata ctaaaatcaa aaacattttc ctcaccataa
    21361 atatcagaaa gtttctttcc ttcttcaaac atagctctta taacagaact atttgcaact
    21421 aaagtttgca ttttattaga tatcatatgt aacactcctt tttatagatt tatattgctt
    21481 atgtatatat taccatattt ttactgaatt tagacaattt tcgaaaaatt acttgtttga
    21541 aaatataaac ttttatataa aatatattta tgtacagtgc ata
//
LOCUS       DAFKMX010000053        21220 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFKMX010000053 DAFKMX010000000
VERSION     DAFKMX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.053"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            164..865
                     /locus_tag="LZB08_001589"
     CDS             164..865
                     /locus_tag="LZB08_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBM7315096.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLD
                     IAMLIGMFGTQIILS"
     gene            891..1547
                     /locus_tag="LZB08_001590"
     CDS             891..1547
                     /locus_tag="LZB08_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315097.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            1703..3343
                     /locus_tag="LZB08_001591"
     CDS             1703..3343
                     /locus_tag="LZB08_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBM7315098.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3701..5023
                     /locus_tag="LZB08_001592"
     CDS             3701..5023
                     /locus_tag="LZB08_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315099.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5220..6227
                     /locus_tag="LZB08_001593"
     CDS             5220..6227
                     /locus_tag="LZB08_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBM7315100.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6265..7692
                     /locus_tag="LZB08_001594"
     CDS             6265..7692
                     /locus_tag="LZB08_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBM7315101.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            7836..9101
                     /locus_tag="LZB08_001595"
     CDS             7836..9101
                     /locus_tag="LZB08_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315102.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9143..10741
                     /locus_tag="LZB08_001596"
     CDS             9143..10741
                     /locus_tag="LZB08_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBM7315103.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            10776..11798
                     /locus_tag="LZB08_001597"
     CDS             10776..11798
                     /locus_tag="LZB08_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBM7315104.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(12186..13589)
                     /locus_tag="LZB08_001598"
     CDS             complement(12186..13589)
                     /locus_tag="LZB08_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7315105.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            14456..14965
                     /locus_tag="LZB08_001599"
     CDS             14456..14965
                     /locus_tag="LZB08_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBM7315106.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            14993..15187
                     /gene="rpmI"
                     /locus_tag="LZB08_001600"
     CDS             14993..15187
                     /gene="rpmI"
                     /locus_tag="LZB08_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBM7315107.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            15243..15599
                     /gene="rplT"
                     /locus_tag="LZB08_001601"
     CDS             15243..15599
                     /gene="rplT"
                     /locus_tag="LZB08_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBM7315108.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            15909..16454
                     /locus_tag="LZB08_001602"
     CDS             15909..16454
                     /locus_tag="LZB08_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315109.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            16889..18814
                     /locus_tag="LZB08_001603"
     CDS             16889..18814
                     /locus_tag="LZB08_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7315110.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            19215..21119
                     /locus_tag="LZB08_001604"
     CDS             19215..21119
                     /locus_tag="LZB08_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7315111.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7988 a         2383 c         3998 g         6851 t
ORIGIN      
        1 ttaagtatta atgtttttag tgagacttta agacagataa tctcctatta aagatactat
       61 atcttagata aaataaatta aaacaatata aaaacataac aagattacag aaagaaagct
      121 cacaataaaa tgctgaaaaa tttattttaa ggaggaataa agtatgaagt ttcaagaaat
      181 gattaatagc ccaggtcttt ggattgtttc tagtttttta gtaattattt cagttgttca
      241 agcaatcgta tttatgaaag aggcactaaa agaggcttca aatcttggta ttgaaaagaa
      301 aggaattaaa gcagcaattc gctcagctag tgttacagcc attggacctt ctctatctcc
      361 tgttattaca ttattatcac tggtagctgt gattggcgct cctacgactt ggatgaggct
      421 ctgtgatgtc ggagcagcta gaacagagct tggagtaata tctcttacat ccaatttatc
      481 tggggtagaa gtaggtagtg ctgcatttgg agcagaagct ttttcgtatg cactatgggg
      541 gatggcttta aataacttag gttggctatt tgttgtattt attttgggtc atagaatgcg
      601 tggaattgtt gaaaagatga acatgaagta taatcctact tgggttaaaa aactacttgc
      661 aggagctaca gttggattat ttgcttacct tctttcaaat caaataaaaa catttgaaat
      721 accaaaactt acaccagcaa ttatttcagc tatagttatg ctagtattga caactttatt
      781 taaaaagaat caacgattac aagaattatc cttagatatt gccatgttaa ttggtatgtt
      841 tggtacacag attatattat cgtgattaaa acggataaga ggtgataatt atgtatgata
      901 aaaagatgat aaaaatagga agaatttcta taggtcttgc aattattgca aactttattc
      961 ccgctatcta tgtaggtatt cgctttggtc agatgccaat gctttctgtt atatttcaaa
     1021 tttgggggtt agtcgctgcg acttatgggg ttagttggat tgttcaacct atagcttatt
     1081 atcctacact aggagctcca ggttcttata taggttggct tgcaggttct gttggagata
     1141 tacgtatgcc tgcagcatca atggcacaga aaattgcagg tgtagaagca ggaactcatg
     1201 agggtgaagt tgttggaaca attggtacat gttgttcaat ctttgtatca gcttctatga
     1261 ttaccatttt taccattgta ggctataaag taattccttt gttacctgaa tttgttacta
     1321 attcatttaa ttatatactt ccagcattat ttggagcaat ttatgttgat attgcacgta
     1381 aggatattcg agctggtgga tgtacgattg ctgtagcgtt agcaattatg tactttggtg
     1441 aaaaaattgg tataccaggt ggattattaa cattgtttat tgtgttaggt gggatactta
     1501 tcacaagggt cttctttgta attgataaca ataaaacaaa agaataggat tgcttattaa
     1561 ggagaaagac tagaattatt tgttgagaaa aagaatagga ttgtttatta aggagaaaga
     1621 ctagaattat ttgttgagaa aaaagaatag gattgtttat taagaaatat gaatataaat
     1681 aagtcttgaa aggagaaaaa gcatgtttga tgttaaaatt gtcaatggaa taatcgttga
     1741 tggtacagga aattcaagat ataaaagtga tgtagggatt gtaggagaca aaatcatggc
     1801 tattggagat ttaagtcaaa aagaggctaa agagactata gacgcaactg gaaaatttgt
     1861 atcacctggt tttatagatt tccatactca ttctgattta tctcttgtgt atgataaata
     1921 cacaagaagc cgtatccata caggtgtaac aacagatgtt attgcaaact gtggtatagg
     1981 agttgctccg ataagagaag agaagaagca agagttaatt gattatcttg gaactaggat
     2041 aattggtaca attccaacaa aattagagct tcattggaat acaatgcaag aatactttga
     2101 ttacttaact gaaaattctc cagcagtcaa tgttgtagct tatgtagcac aaggacctgt
     2161 acgtatcaat gaaatgggat tttcaaagga accagctact ttagagcaac ttaaaaatat
     2221 gaagttggaa gttagaaaag ctatggaagc tggatgtgta gctctttcat caggattagt
     2281 ttatttacca ggagcataca caaagaaaga agaaatggct gaattatgta aggaattgat
     2341 accatacaat ggatactata tatctcatat tagagatgaa ggtgatgaag aaatggaagc
     2401 tcttgatgaa gcaatttata tagctaagac tgcaggagtt cctctacata tttctcattt
     2461 aaaagttatg ggtcataaga attttggaac tattgatgag gtatttaaaa agctagatga
     2521 agctgaggca gatggtttgg aagtaacttt tgattgttac ccatatacag ctggaatgac
     2581 atctttgggt gcacttttac ctccttgggc ttttgaaggc ggtgtagaaa atatggtaaa
     2641 acgattagaa gtacaagaaa atagagacag aattataaaa gagttggagg aagggattcc
     2701 tggatggcaa tgtttctacc aattagcagg tgggtggaac ggtgttgtac tagcctctgt
     2761 aatgactgaa gcaaataaat atgtagaggg taaaacttta atggaggtag ccaaaatcaa
     2821 tggtgaaaat ccatttgata cgttctttag gcttttgatt gaagaaaaaa gtaaaattca
     2881 agtagttgtt catacacaag gtcaagagga tacagataaa gttgtttgcc atccaaagtc
     2941 atgtataggt tctgatagta tggacctttc tacagaaggt ttactttctc ttggaaagcc
     3001 tcatcctcgt gcatttggta cttttggacg aattttctct tactatgtta gagaaaaagg
     3061 tatgttgact tttgaggaag cagttaaaaa gataacttat ctttcagcaa aacgcttagg
     3121 catttataaa gaaaggggtc tacttaaaga aaattatttt gctgacattg tggtatttga
     3181 cccagataca atagaagata aagcgactta tagtaatcct aaacagtata cagtgggagt
     3241 ggaatatgtg ttggtgaatg gtaaaattgc attggcagga ggaaagcaaa ctgatgtatg
     3301 tgctggacgt gtaattaaaa atcctcttag catagcaaaa tagaatacat taagaacttt
     3361 tataaaaata gtctttcttt gaatattaag gaaggctatt tttgttaaat taataaagat
     3421 tactctactt gtgttttcta aagaatttaa ctttaagtag aaaaaacaat atattaattg
     3481 taaaatgtaa ttaatattaa tgaataagat ttaaatacaa aaatttaaat acaaaaatgt
     3541 ttcaacagat agtcgtaatt aaaaaactta aagtaacttt ttatattagg tataattgat
     3601 tattcaaaat tctatgaatc aggagtcctg tggctttagt catggtaggt ttagaaaatg
     3661 ttttaagaga taatagtatc attaggaaag gagagacgaa atgaaaatac tttatgtagg
     3721 accaggtatg gtaatcgata aaatactagc agtaagtaaa aagaattttc ctaatattga
     3781 agcagatttt cttagatatg atagatatat tgaagtacct gatttattgg aaaaatatag
     3841 aggtagagtg gatgcagttc tttttacagg taagtcgcca tttaaatttt ttgaaaaaag
     3901 acaaaaagat tttgtattgt ctgattatat tcctagacat gaaacaacat tgtacagagt
     3961 gttgcttgaa attacatatt tgcttaaatt cgatattagt agacttagta tagatactta
     4021 tgataaggct atgatgaaga gattgtataa agaaattgga gttgaattta aggatgaaca
     4081 tatgtttttt gcagagcaaa agtatttaga agaggactat ataaagtttg tgcttgaatt
     4141 tcataaatat aattatcaaa ataatgatgt atgttgttgt attacaggtc ttgaagaggc
     4201 ctatcatata atgaaaaaag ataatattcc agtagtttta gccttgcctt ctgaggacgt
     4261 aatggtgcaa agtataaaaa acattcagat gcgttatatt gcaaagaaaa acaatgagaa
     4321 tcagattgtt gttcttgcta ttaaactgaa catgccttca gaatattcac taatgaagga
     4381 agatgagtac gcttatcttt cacaaaggat taagattctt gataaactat accatttcaa
     4441 tagccgtata gatggtgttt tggtagagca gagtagaagt gaatttatga tttttacaac
     4501 taaaaaaata cttgagttag aaacaaagaa atacagagat atttatttgt tggatatgat
     4561 tcgtgaagtt tcagttataa acacttatat aggaattggt tatggaaaaa cagcaaatga
     4621 agctaaattt aacgcttatg agagtattaa gatggctcaa agtcaaaaaa atgatgcagc
     4681 atatgtagtt tttgaaaatg gagaaattat gggaccacta aagccaaatc ctactgtcaa
     4741 agagaaaaat agttttgatg aacgttttta tcaaattgct acagagacag ggctaagtgc
     4801 taatactgta tataaagtat tcaataaaat aataaatagt ggaaaaactg aatttacatc
     4861 aaaggaatta gctactatat gtggtgtaag tattagaact atggacagga ttatacttag
     4921 attgtgtgat tctggttatt gtgaggtagt gagtgaacag cttatgagca agtatggtag
     4981 acccagtaga atattacgtt ttaaaccttt tctgttgttt taaatagata taagtcaaaa
     5041 attaaatttt tatgataaca ttttctttac aattttttag atatatgtta atatttagaa
     5101 aaatgcatat gtattcataa aataaactta aaacagctaa aaaatcatta aaaaggaaat
     5161 cgtatgcata aaatgagaaa gttaatttat aacttaaata aatctgggag gagataatta
     5221 tgggaaaata tcaggattca aaaaaagttg taagaaatta ttttgaagca ttagaaaatg
     5281 ctactgggga tgaggttgaa aatgtcttaa agcaacatat gaaatcagat tataactgga
     5341 aaggtgtgta tccatttcgt gaacaagaag gcactgaaaa tgtagcagat gtattttgga
     5401 agcctttaaa aaaatcattg agtaatatgc aaagacgaca agatattttc attgcaggaa
     5461 caaatgagat tgacaatagt gaatgggtaa tgagtatggg gcattttatg ggactttttg
     5521 ataaggattg gcttggcatt aaacatactc gcaaaatgat tagtttaaga tatgcagaat
     5581 ttaactgtgt tcaagatggt aaaattgcta aaactggatt atttgtagat ataattgggt
     5641 ttatgattca agcaggagtt aatccattgc caccacaaac aggttcatat tttgtatatc
     5701 cagggccaat tgaccataat ggattacttt ttgaagatgc agatgaagtt gaaggagtaa
     5761 aaactttagc attagtaaat aaaatggtag atgatttatc tgaattaaat gaaagtggtg
     5821 ctatgggatg cccaccagaa atacttgaaa aatcttgggc taaagatatg atttggtatg
     5881 gtccaggtgg aattggtgct tcttatacta tacctagata tcaagagcaa caccagctac
     5941 catttagaaa taatttaaaa ggaaagactt ttaatgggca tgtatgtaga tttgcagaag
     6001 gtaattttgc ttgtttcttt ggatggccta atctgactaa tgtaccatgt ggaggttttt
     6061 taggattacc aggtggagag gttaaagcag atatgcaagt tgttgatgtt tattatcgta
     6121 agggcgataa acttcaagaa aactgggtgt taattgatat tccttattgg ttgaaacaac
     6181 aagggcttga tattcttgag agaactaaaa gtatttttaa tgcttagtta tgcatacgta
     6241 ttctaaaata aagggggata taaagtggca tctaccaaca aaaataatgc aacaagtaaa
     6301 gaagaagaaa aagaagtttc tacaagtggg cttgaatcat gtacggttaa agaaaacaaa
     6361 gctaaaatga atcaaactaa ttttagtaat ataaatgaaa atcaaacaaa tgagaataaa
     6421 acgaacacat ataaaagaaa tatagataaa ggaaatatag ataaagggaa tatagataaa
     6481 acaaacacaa ataaatccga cataaataaa gttaacatag atacagtgga tacagataaa
     6541 ttaaatacaa atactcttac tgaaaaaaat aaaaaagtta tatttaaggg ggaagataat
     6601 atgtcatcta caaaaattta ctatggagat tcattagaag taacgcctgt tggagtaatg
     6661 gattacaatg atttttcaaa acagacaaaa agggaacagg aaatacctgg atttgattct
     6721 aagtatagag attttgtaga ttatattatg aagattacac ataatatctg ggaagaaaaa
     6781 ggtataggtg ttatttatga tacatatcac aataatgtta caatgcattg tggctcttca
     6841 aatttagttg gtattaaaga tgttatttcc aatactcttc aaacattaca tgcgtttcct
     6901 gatagaagat taattggtca aaatgtaata tggtctaact ttggtgctga tggtttttta
     6961 tcttctcacc gtgttttgtc tacagcaact aacttaggtg atagtaattt tggaccagca
     7021 acaggtagaa aaataaactt tagaactgta attgattgtg ccacaactaa caatagaatc
     7081 catgaagaat ggttggttag agataattta tggattgtta ctcaattagg attaaaccct
     7141 caggaagtag caaagagtat ggcaaaggca agtgaatcta aagtgcttag tttacaatca
     7201 acttatggta tctgtgaatc tatggatggt caatttatgc caactaagta tcaagcaaaa
     7261 gatgattctg ttggcgaaat gatgcttgaa atgacaagcc gtatttataa ttataaatat
     7321 ataaatgaag ttaaaaaata ctatcatgat aatgcagtag tacattttat atgtgataaa
     7381 gatttaaatg gttatgatga gattcaagga atgattgtaa gtttacttgc atcaattcca
     7441 aatggaagtt atgaagtgga aagagtaact tgtaatcaaa gagaaaaaaa tgaaggttat
     7501 gatgtatctg taagatggag attacgtgga ataaatgaag gtataggatt ttttggacag
     7561 ccttctggaa agcatataga agtaatggga atcaatcatt atcatatact acaaggtaaa
     7621 gtaaaggagg agtggataac ttttgatggt atggatgtat taaaacaaat gtatatggga
     7681 gtagaagaat aaagctgttt agagtataaa tagttttgaa atgtatatag ggaagtattg
     7741 ctttataaaa tttaagtggg gaatgtatat gaaaaataga tgtttaaact tttagcaaaa
     7801 ttatgactaa atttaaaagg gggaattata aaaatatgaa aacaacggtt gaaatagaaa
     7861 atgatatcat attaggaaaa gactattcaa acctaaaaag aggaattgca tttttttcaa
     7921 ttgctttaat atactttttt tattgctaca attttatggt gggtactttt attaaaccaa
     7981 caatgattta tgctttagca gatgggggat ttggtttttc attaaaacaa acagaagaaa
     8041 tatttgcagt tatgtctttt ggtacaattc caggtacatt tatttttggg gtgatttcta
     8101 caaaaattgg gaagaagagg actttaatta gcgttgcttt actgattgga ttgacaacat
     8161 ttataccaat gttatctcct acaaatatta tgttatggaa aatagcacgt ttgtgtacag
     8221 gagtagtgtt gggaggcgta tttggaacag ctatgccttt ggttgcagat atgtttccaa
     8281 gtaaatatcg tggtaagtta gctgctatat taactgcttt attttcatta gcaatgattt
     8341 ttggtgggaa agtatatggc gttttaggtg atgcaaattg gcaaatattg atgtatactg
     8401 cgattatacc acctatagtt ggagctatct tagcaatttt ctttgtgcca gatgatttgg
     8461 agtatacaaa ggaattagtt aaaaagggaa aagaaactgg tgaaaagatt agctatataa
     8521 gtatgtataa gggaaaatac ttatggattg gattgggaac gattctttta tcaggtgcaa
     8581 attttacagc atattcagca ttttcaaata atgcaactac atacctagtg acaggaatag
     8641 gaatgtcagc agcagtggct gggtctatat atagtttaca aggaattggt caattagtag
     8701 gatatttatt ctggggttca attgcagata aatttggaag aaaaatacct tttataggta
     8761 tggcattagc agcagtattt gtttttatat atacaaaatt aggtggagat aatacaacaa
     8821 cattctatat gatatcagta ttactaggtg ttgctgttgg atattctgga gcatggggag
     8881 catactacac agagttgttc ccaagtaaat atcgttcgtt gtcatctggt atgtcattta
     8941 atggaggaag gattatatct acgtttgcaa tacctgctat agctggtaca gcttctggta
     9001 ctttaggtat gatgactatc ttttacatat caatcgcagt ctttgtagca ggtgctattg
     9061 tatggttatt cttaccggag acgttaaata ataaaaaata aaatactagg aatttatttt
     9121 gttacctaga aaggggtggc ttatgatttc atcaattgat gtatgtatta taataggata
     9181 tttgctatta atgttagtta ttggttatta ttcaggaaaa gataataaaa atcaagaaga
     9241 ttatttcttg gcaggtaggt caatgccatg gattccaatt gctttatcag ttgcagctac
     9301 tatgattagt gcaaatggct ttataggtgg tcctggatgg gcatatgtag atggaatgta
     9361 tccagtaatg gttaatatta cagtaccttt agcaatattt tttgcactga gcattaccac
     9421 cccagttatc tataaattaa aaataacctc tatatatgaa tatatgggac ttcgtttagg
     9481 aaattattca agaggattaa ctattattca attttttatt aattcattga ttcaagctag
     9541 ttctatggta tatattccag tgcttattat tcaaatgatt actggctggt cactttatgc
     9601 tttagtccca atagttgtac ttgtatcaat tatatatact ctattaggtg ggattaaggc
     9661 agttatatgg actgatagta ttcaaatgat agtagtaata agtgctgtat ttatagtaat
     9721 tattgtagcc ttaaaaggaa tgaatctaag tttctttgat acattacaaa ttgcacaaca
     9781 gagtggaaaa ttaaatacat ttgatttcag tagggatatt tcagtgacaa atactttttg
     9841 ggctacttta attggtggaa ctgtaatgtg gataagatac ttttgttttg accaatctca
     9901 agtacaaaga gttttaacat ctaaatcttt aaaatgtgca aaaaattctt ttgtagttag
     9961 tgcatttgtt atgaatttag tttactactt tatgctattg gtaggagtaa ttttatttgt
    10021 tttttatggg ggaaggacat ttgaaacttc aaatgaaata atgattactt ttattttaaa
    10081 tgaacttcca gtaggagcca ttggaattat tattgcaggt gtgtttgcag cagcaatgtc
    10141 tagtatcgac tctatattaa acagtatgac aactgttttt acgaaggata tttatgagtt
    10201 ttatttcaaa aaagacaaca aagaatcatc attaaaagtt acaatgatta taactgtagt
    10261 tataggagtt gttatgacag gttttattat tatgggattt ggaggaagca tcaaatcaat
    10321 tcttgacctt gttggtaact atatctcata ctttgctgga ccagctacag gtgcatttgt
    10381 actagcaatg tttacatata aagctaatga taaaggagta tctattggtt ttattgtagg
    10441 gttagtttta ggatttttta tttctaataa atataatgtg agttggttgt ggaatccagc
    10501 aataggtgct acaataacat tgatatttgg atatattttt agtgtcattt taaagaaaaa
    10561 tcataatgca gatgatatta aaaaatacac tgcatttggt atgagaaatc accttatttc
    10621 tcaaggaatg gataaagtag atggagtatc tatagtgcca ttttctttag gtaaacaaga
    10681 actaattgta ttagcttttt ttattatcca atatattata ttatttttga tacaatttta
    10741 agaagaaata ctagatattt aaggagaaat actaaatgaa tcaaaagaaa gttcattata
    10801 ataaatcggc tacctcaaaa gatgttgcaa gattggcagg agtatctcaa tcatctgttt
    10861 caagagcatt tggtagtgca aatggaaagg gcgtaaaacc tgaagtaaga gagagaattt
    10921 ttcgtgtagc aaacgaaata ggatatgttc ctaatttggt agcacgggga atgattagtg
    10981 gaaaaactaa tgttatagga ttgatagttg gtgatagtct aggtccattt tacaatagaa
    11041 ttatcaattt atttgtagaa aaaattcaag agataggtaa acagtgtctc gtttttaaag
    11101 ttcctagaca acaaagtata gatagtataa tttcaaaagt aattcagttt caagtagaag
    11161 ctgtgattat tacagcttcg gccatgaata aggtgatggc tgagacttgt gaagaaaaca
    11221 atattcctgt gattcttttt aatcgtttta tacctggtat aaaaataagt acagtctatg
    11281 tagacccaat tgaaggtggg ggaatggctg ctgaatatct gcttaagaaa ggtcataaga
    11341 atataggata tattcaattt acgagagaaa caagtgaaga aatggaaaag aaaattggat
    11401 tttattctaa gttaagacaa agtgggattc ataaccttaa agaagaatct gcaaactatg
    11461 attatgattc tggatatgaa gcaggtaaga gaatgttagc attatcaaat cctccaacag
    11521 caattttttg cacaagtgac ttgatagcta ttggtgtaat ggatgctgca aggtttgagt
    11581 acaagttgag ggtgccagag gatttatcag tgataggata tgatgatatt caaatggctt
    11641 catggaagag ttataattta acgacgatta gacagccact agatttgttg attgaaaaaa
    11701 caattaacat acttaagtcc ttattaaatg aagaaaatcc agagtcagtt gttgaaatgt
    11761 taaaacctga attgattgaa agaggttcta cactttaagt ggtagatatt taattaagtg
    11821 gtagatacct aattaagttg tagatatctg ataagaaatt tttaagtttt caatcgttct
    11881 aaattgtgag tataaaaatc tactaaattt agttatgtaa aatataaatt ataagtgtag
    11941 aaactaggct actagctttt ataccacttc aaacattact ggaggtagtt aagtttacat
    12001 cagtttagtt ttatgtagat ataatattct gtaaatagac aaaattaaaa tatgtaagtg
    12061 tctcaaaatg aaatttttag tttatgaggc acttttttgt ataaaatcaa atgtattttt
    12121 ataactttaa caataaaaag aggcttatct ctattttgag acagcctcaa atttttaaaa
    12181 ctatattagt ttacagcttc ttttcctatc aaatctttta actcttcatg agtcatagtt
    12241 tctttttcaa ataagaattt agaaacagaa tgtaaatctt caagattatc ctttaacatc
    12301 tctaaagtct ctttataaca aatttcaact atacgatttg cttccttttg aaccttatca
    12361 cataagaaag tcttatcatt tccatcaata gtcataaatc ctaagttact cataccatat
    12421 tcacaaacca tttggttagc tatttcagtg actttcttta agtcgtcctt tgcaccagta
    12481 gatttatgat taaatattaa ttcttctgaa gcctttccag caagtaatat ttttattttt
    12541 ccaatcaact catccttagt ataaatatat ctatcttcat caggtaattg aagaacataa
    12601 cctaatgctt gtcctctagg aactatagat attttttgta taggacaaat tcctactata
    12661 tcacttacaa gagcatgtcc agcttcatga tatgctacta tttttttctc tttttcaacc
    12721 aatgcagagt ttttagattc tagccctgca ataactcttt caagtgcctt gtcaaaatgt
    12781 tctgatgtaa tacactcact gtcatctcta actgcaaata tagctgcttc atttgctata
    12841 tttgaaagat gagccccatt gaaaccatga gtttttttag ctaaaagttc tagatttaca
    12901 gatttgtcta taggtttgtc atctgtatgt acctttagaa tttcaaatct agtgtgatag
    12961 tttggtgcac ctatatgaat gtgtctgtca aatcttcctg gtctaagtaa agcttcatct
    13021 aataagtcaa gtctattggt agcacctatt attaatacat tggaatcttt attaaatcca
    13081 tccatttcaa ctaaaagttg gtttagtgtt tggtcttttt cgttgttact ttcaagatgt
    13141 ctctttgctc ctacagcatc tatttcgtct ataaatatta tacttggagc ttcttttcta
    13201 gctttttcaa atagagttct aactcttttt gctcccacac ctacatattt ttcaacgaac
    13261 tctgaaccag taacattgaa aaatgatgag tttgtttcac cagctacagc agatgcaagt
    13321 agggttttac ctgttcctgg aggaccataa aataatactc ctttaggtat ttttgcaccc
    13381 attttttgat atttttgtgg tgacttcata aaatcgacta tttcaaatag ttcttctttt
    13441 acttcatcta atccagcaac atctctaaat gtagtctttg gttttgtaga aagtgaatca
    13501 tctgtttgat tatctctttc catttttgtc tgtgcaaaga caggagctgg attgttgatt
    13561 ttcttaaaaa agttgtttag cattttcata ttcacacctc aggtaattaa ttattgttaa
    13621 tcaattttat attttccata tttactattt tttattattt atttttcatg gaaaaaatat
    13681 acatgttatc taaataaaaa taaatttgta agatgtatga aaaaatgaat ttatgtatat
    13741 agagatttct acttatgtat tgaataaagt attacaaatt ataaattatg tattgattat
    13801 atattgagta aagtattata aagcatgtat tgattaagta ttgaataaaa tattacaaat
    13861 ttagttgaaa tgaaaatgat aaaaaatctt gtactatatt ttacaaatca tataaataga
    13921 gaaattgttg tagcacaaag tagtaggtag tatattagaa tataatatag gatttgttgt
    13981 gtagtatagt agcatattag aatataatat aggatttgtt atgtagtata gtagtatatt
    14041 agaatacaat atagaatttg ttgggtagta tagtgttata ttagaataca atataggatt
    14101 tgttatgtag aatatataga gtcatgtaga atataaatat ataaaaggat tttgggtata
    14161 aatttaaaaa ttaatagcaa aatgagtagt atagacatat aattagtagt aggaagtaat
    14221 taaatttttc ctaaacaaaa aaaatagttg caaataattt gattaagtga tatagtagat
    14281 aaggtaagaa taaaacaaag aagaagtccg cttctcacct tacgccggag gttagtaagg
    14341 taaatataag ttaaagctta attttaattt aagtttgttt aaatttggta acttatattg
    14401 cggatgatta cgtcatccgc tttttatttt ataaaaattt tggaggtgtc ctacaattag
    14461 caaagaatta gcaatcaatg aacaaataaa agataaggaa ataagagtcc tttcaccaac
    14521 aggtgaacaa cttggagtaa tgcctactaa agaagctcaa gcaatggcta atagcaaaaa
    14581 cttagatttg gttcaaatct cgccgaatgc aaatccacca gtatgtaaga taatggatta
    14641 tggaaagttt agatatgagc aagcgagaaa agagaaagaa gctaagaaaa aacaaaaaac
    14701 tatagttgta aaagaagtaa gacttagacc aggtattgaa caaaatgatt taaatacgaa
    14761 agctaataat gctataaagt tccttaaaaa aggggataaa gtcaaagttg aacttagatt
    14821 tagaggaaga gaattaggac ataaagacat aggtaaagaa gttatgctta agtttttaga
    14881 tataataaaa gagtttggag aaccaacaaa agcccctgca tttgagggta ataatatggt
    14941 tgtaattata gatccaaaaa aatagatgat taactgagag gaggaatttg tcatgcctaa
    15001 aatgaaaact catagaggtg cagcaaagag attaaagaaa acaggaactg gaaagttaaa
    15061 gagagctaaa gcttttaaaa aacatatatt aactaaaaaa tctgcaaaaa ctaaaatgaa
    15121 cttaagaaaa tcaactttag taagtgatgg agatgctaag agaatagcac aattattacc
    15181 atactaagat acagattgta aataataact atagaagagg gatataaaag gaggtcataa
    15241 cgatggcaag agttaaaaaa gctatgaacg ctcgtaaaaa gcataagaaa atattaaaac
    15301 ttgcaaaagg attcagagga tctagaagca aattatatag accagcaaat acattcgtaa
    15361 tgaaagcatt aaaaaatgct tatataggaa gaaagttaaa gaagagagac ttcagaaaac
    15421 tttggataca aagaataaat gcagctgcta gaatgaatgg aatatcttat tcaagattaa
    15481 tgaatggatt aaaattatct ggtgttgaag ttaacagaaa aatgttatct gaaatggcta
    15541 tacaagatcc agaaggattt gcaaaattag cagaagttgc taaagcaaaa ttagcttagt
    15601 attgaatcat gagatgtaaa gtctttgagt tatataaata attcaaggac ttttttattt
    15661 tttgaaaaat acttattagt gtcatgtaca accaatttta taatgataaa taaaataagc
    15721 aatacaatag aattttatga gatttattat aagaattgtt acaattattt atgatagcta
    15781 taaaaagtca tagtaaaaat gatattgatg tatgttgata tatgtataaa gtttccaatc
    15841 aatcttgaat taatattatc aacatgataa tattatctta ttaataatat aaataagggg
    15901 gacataatat gaatgaaaaa ctaatggatt gttttgtaaa cccaattaaa tgcaagttat
    15961 tgattgaaat tttttcaaat aatcgaacta cagctaaaca attaggagaa aaatttcaag
    16021 atatatcaaa ggcaacactt tatcgttacc ttaataaaat gttgaaagat gatattataa
    16081 aagtggttga agaaaataaa ataaggggaa ctgttgaaaa agtatatgct atgaagttag
    16141 atatagctga tgatgtgaac aaaattatag atgagaactc tggagaagaa tatgctcaac
    16201 tgtttactca atatatgatg ggatttttaa gagaatttta tgaatacgct gaaagaaagg
    16261 aaaaggatat cctaaaagat ggttcaggat tttctgtctg tccagtatat gctagtattg
    16321 atgagatagt tgaagtgagt aaaaaaattg ctagtatact agaacctcta agaaataact
    16381 cacatgaaga aggtagaaga atgcattcta taggagtgat aattactcca cctaaaaagg
    16441 atgaagggaa ataaatctga tttacatggg gtagagccaa tttatacagg gaatcgtttt
    16501 atgatttaca tatgtatcca gagcaaagta tctctgcttg gattgtactt cattgtacca
    16561 tttcatccaa tgacttggag tggtacaatg aagtttttta atttttcata ttaatgctaa
    16621 agaaatgaac tttagaccac atatgcaatt aggttgcatt atacaattag accacatata
    16681 caaaataaaa atcagtattt ttatagcaga taaattgact gttattcttc ttaaaaatca
    16741 tctattagtt attgttagca tattgaaaga aattatagta atctattgaa taaatgaata
    16801 ttgcccataa tcatataaac tgctatagtt taaaataaag caggacgtta aattttatag
    16861 taatctaaaa atttaagagg gggattttat gaaaataaaa tttgttatta tgccattggt
    16921 gatattttta atggtattat ttgtggggtg tcaaagtaca aaagatacag aagttaaagc
    16981 aaaagataca gaaattaaaa tattaggtac aggtgatttg cattcaattt tgacagactc
    17041 tatggtttcg tatgtaaatg aggaaaggga aaagaataaa aatctgctta tggtagatgc
    17101 gggagatttc tatggcacac aaagtagaga aatgtgggaa tggtcttctg gaaaaaagtt
    17161 aattaatatt aaaagaaatg gtagagcaga gtatgatgac ataattaaga gtagtaaaga
    17221 tgaagtacct atagttaaag atatggcaaa gttgaaatat gatgctgtag ttttgggaga
    17281 taatgaattt gtttcaaatg ataagcaaag tcttgataaa ctagtttctg attttaaaaa
    17341 taataatatg ccattggtat cagcaaatat atatgaacaa tctggagaaa attatgtaca
    17401 accatatgta atgaaaaata ttaagacaga tgagggcaat gtaaaagttg gaatactagg
    17461 tcttactata aaagaagtag gggaaaccct tggtcttgag gattttgaaa aggataaaaa
    17521 agctagagag cttggagaac aagctgatta caaaggaaaa ttatatgcaa atgatttagt
    17581 agaagatgca aaaaaatgga taaaagttat ggaaaaagaa agtccagaca taatagttgc
    17641 agttgtacac tcaggggaag aacctaaaac agatagaaat ccaggaaata gaataaaaga
    17701 gttagcaact acagtggatg gaatagatgc aattgtagct ggtcatactc atgaaaaaat
    17761 agatgaacac aaatataaaa ataaatctgg agatgaagta attgttacac aaccaggttg
    17821 gcatggagat agagtatcag agattaattt caaattaaat aaagagaatg gaaagtggga
    17881 cataaaggaa aagtctagta aattaaaaat aattgataaa gaagtaagca tagttgctac
    17941 tgcagattta cattcacatt tctctgataa attaacagtt gaccttgtta atgaacgtag
    18001 taacccaatt gaacctgttg tagtagatgc aggcgacttc ttagaccctc aaacagatga
    18061 aatgactcaa tggtataaag agtggaaaag tataagagat aataatagtg ataaaacgat
    18121 tagtagatgc ccaatggtca tggatatgaa tcagggaatg tatgatgcag tagtacttgg
    18181 aaatcatgaa tttgtatctg aaaaagatga attttggtca gatgaacgcc ttttagatgc
    18241 tgttatagag gattttgaac aggtggcaat ttctgtgtta tcggcaaata tatataggca
    18301 atctggtaaa aattatgtta aaccatatat tataaaagat gtggagacga gtgaaggtaa
    18361 tgtaaaagta ggaatattgg gacttactat aaaagaagta ggtaaaggtc ttaaaaatgt
    18421 aaacttacaa gagcaatttc aatataagga taaattgtat gcaaatgatt tagtggaaga
    18481 tgcaaagaaa tgggtaaaag ttatgaaaaa gaaaaaacca gatataatag ttgcagttgt
    18541 acattcagga gaagaaccaa aaaatccaaa acatccagga aatagaataa aagaactagc
    18601 aactacagta gatggaatag atgcaattgt tgctagtcat actcatgaaa aaatagatga
    18661 gcatgaatat aaaaataagt ctggagaaaa agttatagtg acacaaccag gtgagcatgg
    18721 taaatattat tctaaaatta cctttaaagt aaataaagaa aagggttcgt ggattattaa
    18781 tgataaattc agtaaaacaa ttgaggttga ataactttat aaaatttata gataaagtaa
    18841 attatctgat attgacatga tatataagat aaagtttcta atattagtat aatgcataat
    18901 gtgaaatatt ttaataataa gagaataaac aagacaaata aaaatatttt aacatgtaag
    18961 atatagtatg aactattata ctaatgctta ctttataatt tgaacatatt agaataagtc
    19021 ttatttgaaa attttagtgt attagagtaa gtattatttt accagtaaaa aatattgaga
    19081 actatatgta gatttgctta aaaaggaatt tacatatagt tcttttcgta taataaatag
    19141 tacaatctta tatggtaaag ataaagtgtg atattaaatt ttattatcat ccaaaatcta
    19201 aggaggagtc tcttatgaaa ataaaattta ttatagtagc atcaataata tttttaatag
    19261 tattacttgt ggggtgtcaa agtactaaag acacagaaat caatgtactc gccacaagtg
    19321 atttacattc aattattcca gataatttaa tttcctatgt agaagaggag agaaaatatg
    19381 atgagaatct acttatggta gatgcaggag atttttttga catgcaaagt ggtgagatga
    19441 ataagtggtt tactggtcaa aaattagtaa gatttaaaga tgggatacca gagtacaaaa
    19501 aaatagctga accacgtgaa ggagaagttc cattggctaa aaaaatggca aagctgaagt
    19561 atgatgcagt tacatttggg aatcatgaat ttgtatctaa tgacaagcag agccttgata
    19621 aattggtttc tgattttaaa aataacaata taccattagt atcagcaaac gtatatgaac
    19681 aatctggaga aaactacgta aagccatatg taatgaaaaa tataaatact gacaagggaa
    19741 ctgtaaaggt tggtatactt ggtctgacta taaaagaagt aggggaacgt tcaaggttta
    19801 aaagttttga acaagataaa aaggctagag aacttgggga acaacctcaa tacaaaggga
    19861 aattatatgc aaatgattta gtagaagatg caaataagtg ggtaaaggtt atggaaaaaa
    19921 aagaaaagcc agatataata gttgccattg tacactcagg tgaagagcca aaaaatccta
    19981 aaaatccagg aaatagaata aaagaactag caacaactgt agagggaata gatgctattg
    20041 tagcaggtca tacacatgaa aaaatagaac agcatacata taaaaataat tctggtgaag
    20101 aagtaatagt aacacagcct ggagctcatg gtaatagtat atcaaagatt aattttaagt
    20161 tggataagaa gaatgataag tggtttataa aagacaaata tagtgaatta aaggtttttg
    20221 gaaaggaagt aaatataatt acaacttctg gtctgaatcc aaaattttca gatgaaatgg
    20281 cagttaattt gtttaatgaa agagatagag atgttcaacc tatctttata gatactggag
    20341 attttttaaa tactaataca aaagaaatga ctaagtgggc taaagagtgg caatacaatg
    20401 aaaataaagg catctataaa aaaataaatg aatggccaat tgtatatctt ggttatgatg
    20461 ctgtagtact tggtagccat gaatttacag aggataaaga gacttttgag gaaaaaaaat
    20521 ctacattgga ttatattata gaaaactttg aagaaatgaa gataccagta ttatcagcta
    20581 atatatatga tgaatctgga gaaaattatg taaaacctta tataattcat aatgtagaaa
    20641 ctagggaagg aaatataaag gttggagtat taggtcttac tactactgat aaggatgaag
    20701 aagacttaag taactcaagc ttaagcaaag actcaaagac ttcgaaaatt cctgaagaaa
    20761 caggaaagtt atatactaat aatctagtaa aagatgcaag cgaatgggta aaagttatgc
    20821 aaaaggaaag tcctgatgtg attgttgcag ttgtacattc agataataat agtactctaa
    20881 aaaatccaag tagtgaagtc aaaaaacttg caatgtcagt agatggaata gatgctattg
    20941 ttgtaggtca tacgaaaaat aaggtagaag aacatattta caaaaataag tctggggatg
    21001 aggttatagt aacacaatct ggagaagatt gttattcaaa aataagtctt gaactaagaa
    21061 aagaaaatgg caagtggaat atcataaaca aatatagtaa agcaataaaa atgaagtgaa
    21121 tttattgata tataaaccat tattaatact gatttttata atataataaa tactgattct
    21181 acaatataat taacactgat ttttataata taataaatac
//
LOCUS       DAFKMX010000054        21172 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFKMX010000054 DAFKMX010000000
VERSION     DAFKMX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.054"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            14..619
                     /locus_tag="LZB08_001605"
     CDS             14..619
                     /locus_tag="LZB08_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315112.1"
                     /translation="MIEKLNANASLSDLITTFENVKDELQIGKNNIAGTLGSPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            643..948
                     /locus_tag="LZB08_001606"
     CDS             643..948
                     /locus_tag="LZB08_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315113.1"
                     /translation="MTEYIEVGRRIFFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDL
                     EYDYIDYDKYKIKGINIETKQPILEEIPIYMSEEEKRIQELENQILIAENEKVGGIL"
     gene            948..1112
                     /locus_tag="LZB08_001607"
     CDS             948..1112
                     /locus_tag="LZB08_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315114.1"
                     /translation="MNINNIVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQKIK"
     gene            1312..2661
                     /locus_tag="LZB08_001608"
     CDS             1312..2661
                     /locus_tag="LZB08_001608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315115.1"
                     /translation="MIEKLNANASLSDLITAFENSTNEIKTISNTLKQTLINKNIKVL
                     DTDKLSSLVDKVDKLKAQEFGVRINKLDENPDTCVSYLGDAIGMTPASVGSYGSWDTI
                     DFIRNIKPCGFKDGIVTKYIKKENFNMYEDGTNVEDDTDVMIEFPKFYWKIQSSDNYM
                     DIFISKTKLDDDYECPAHLIGSTEKEFIYIGAYLASLENNNLRSKKNSSPELRERGRF
                     YSLIYKHVPGYDSLDYQCVLMLQILFVVMFKSINFFRLGVGYSSYISGHGKPDKINTG
                     GSNFKGMIYGEQNGDEQMKFLGIEDLWGNCLQEVGRCTCGVENACVYTRIITRDKRNI
                     QYGVGRKVGTISDVIGGNNTGFMVKQCLPGGGNGQDSRYYRCWANLSENHIGYYTGFD
                     YNENFRYYGLFSSYFQTEVKKASTRLIFIGEDNQNTLKTIQEDELLFVENEKVGGIL"
     gene            2661..2840
                     /locus_tag="LZB08_001609"
     CDS             2661..2840
                     /locus_tag="LZB08_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315116.1"
                     /translation="MIDGVVVRILADRILNKGLNPLKNRVFELDDVTNTEYRIAVENY
                     IIEHSEVVEGAELTK"
     gene            2881..3111
                     /locus_tag="LZB08_001610"
     CDS             2881..3111
                     /locus_tag="LZB08_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBM7315117.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            3131..3388
                     /locus_tag="LZB08_001611"
     CDS             3131..3388
                     /locus_tag="LZB08_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBM7315118.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPIILLVL
                     GIGFSIWMLGFSPNAVLQGIICWGISIGINQTYKQLKDGEK"
     gene            3649..4512
                     /locus_tag="LZB08_001612"
                     /pseudo
     CDS             3649..4512
                     /locus_tag="LZB08_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            4591..5403
                     /locus_tag="LZB08_001613"
     CDS             4591..5403
                     /locus_tag="LZB08_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7315119.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKFLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSKPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINSEGVKQMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGTITKEHYTMIKGNDRFDTLYKALDFINR"
     gene            5690..7699
                     /locus_tag="LZB08_001614"
     CDS             5690..7699
                     /locus_tag="LZB08_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBM7315120.1"
                     /translation="MKWASKKIFILSLSSITILSSVGRVNALEIKMNSNLNKQIEKRT
                     TIPDLDTLTMVQAFPDANFRAYLCDILSIKDENALISKDSKDKIEVIDNLGLYEKSIK
                     DLSGVNYFVNLKRLYCSNNNLKTLDVSNNPELEILSCYGNNLETLDVSNNLKLRDLHF
                     GGNNIKILDISKNKKLEILYCYDNSLKTLDVSNNPELTHLSCFGNNLETLDVSNNPEL
                     KELYCFRNDLKTLDVSDNPELIRLDCSDNMLKTLDVSDNPELIHLDCSDNMLKTLDVS
                     KSPELKELYCYRNDLKSLDVSNNQGLKELYCYDNNLKTLDVSNNLELTHLSCGGNMLK
                     TLDVSRNKNLIILDWENQRKESSGGNSYTPVPIVTKTNYLVGSDRYDTSVKISKEGWD
                     NVDNVVLINSSSIPDALSATPFAKAKNAPILLTQTNSLNQLTENEISRLGAKNIYIIG
                     GFNSVDASIESYLKDKGLNIIRISGNDRYDTSIKLAKELNKENKLSKLVLVNGEKGLA
                     DAVSMGAISAKEKMPILLTNEIDDMKAIEELIDNKDISKSYVVGGDSLFNEDIGDKLP
                     YVIRISGEDRAETNSKVIDYFYDNNVLNNLYIAKNGENREDDLVDALSVGVLAGKTES
                     PVLLVGDGIKDSQKSLINKREFRNITQIGGNGNEKAFSEIENLVK"
     gene            complement(7744..7929)
                     /locus_tag="LZB08_001615"
     CDS             complement(7744..7929)
                     /locus_tag="LZB08_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBM7315121.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKDENE"
     gene            8654..9331
                     /locus_tag="LZB08_001616"
     CDS             8654..9331
                     /locus_tag="LZB08_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBM7315122.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            9505..11541
                     /locus_tag="LZB08_001617"
     CDS             9505..11541
                     /locus_tag="LZB08_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBM7315123.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDENYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(11732..12298)
                     /locus_tag="LZB08_001618"
     CDS             complement(11732..12298)
                     /locus_tag="LZB08_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7315124.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            12586..13521
                     /locus_tag="LZB08_001619"
     CDS             12586..13521
                     /locus_tag="LZB08_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7315125.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            13986..15200
                     /locus_tag="LZB08_001620"
     CDS             13986..15200
                     /locus_tag="LZB08_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315126.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYNLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(15619..16140)
                     /locus_tag="LZB08_001621"
     CDS             complement(15619..16140)
                     /locus_tag="LZB08_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7315127.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            16496..17293
                     /locus_tag="LZB08_001622"
     CDS             16496..17293
                     /locus_tag="LZB08_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBM7315128.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            17403..17993
                     /locus_tag="LZB08_001623"
     CDS             17403..17993
                     /locus_tag="LZB08_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBM7315129.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            18059..19756
                     /locus_tag="LZB08_001624"
     CDS             18059..19756
                     /locus_tag="LZB08_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBM7315130.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEVVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            19970..20809
                     /locus_tag="LZB08_001625"
     CDS             19970..20809
                     /locus_tag="LZB08_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBM7315131.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         8486 a         2166 c         3513 g         7007 t
ORIGIN      
        1 ggtgatattt tttatgatag aaaaattaaa cgcaaatgca agtttaagtg acttaataac
       61 cacttttgag aacgtgaaag atgaattgca aattggtaaa aataatatag ctggtacatt
      121 aggaagtcct tttgttggaa ctgataagct aagtgtaact aaaacaaaaa tacagacctt
      181 gaaaaataca ctagcaatta gtttgtctat gaaaaatata tctgctcaat caactgaaac
      241 acttcattca ctaattggta aagtagctgg aatcgcaaat atttttattg cacaaggaga
      301 atgtactcaa gttggagaaa aaacttttca actctatcaa gtagataata atacgccaaa
      361 aacttgttca gcaaacgcaa taagttttaa cttagctttt actccaaaat ttattattgt
      421 ctatagaaat gcttacaata taggtttgtg tagttttgtt ttttactcat cttttcgtaa
      481 tattataaca gcagtgcctc aacctagcaa ttttggtgaa atgtacatgc ctaacacatt
      541 cgattgtgta gtaggtcaca cttgtatttt accagtgaaa gattacaaaa taccacattt
      601 tttctatgca attgcttaaa aaataagtta aaggaggaaa ctatgactga atatatagaa
      661 gtaggaagaa gaatattttt tgatgaagaa ggcgaaataa tattctatga aggacaatcg
      721 aaaggaaatg ttccagagcg aaaaaatatt aaaaaaatag aatatataga tttggaatac
      781 gattatattg attatgataa gtacaaaata aaaggtataa atatagagac taaacagcca
      841 attttagaag aaataccaat ctatatgagt gaagaagaaa agagaataca agaattagaa
      901 aatcaaatat tgattgcaga aaatgaaaaa gtaggaggaa ttttataatg aatataaata
      961 atattgtagt aagaatatta gcagaaagaa ttttaaacaa aggactgaac cctttgaaaa
     1021 acagaccttt tgagttagat gacgtgacta acatagagta cagaaaagca gtagatgatt
     1081 atataaatac aatagaacag aaaataaaat agtaactttt agtaactctt cttaaagaat
     1141 aggtcactaa aaaattttaa ttttgtttat tttacttact ttcacatact tataaagaaa
     1201 tcattctaaa ttctacatta aaagaaacgt tcaaatttaa tcatctttct actttaaaag
     1261 taacctatcc tttaaaaatg gttactaaga aagtaggaag gtgatatttt tatgatagaa
     1321 aaattaaacg caaatgcaag tttaagtgac ttaataaccg cttttgaaaa tagtacaaat
     1381 gagattaaaa caatttcaaa tacattaaag caaactctaa taaataaaaa tataaaagta
     1441 ttagatacgg acaaattaag tagtttagtc gataaagtag ataagttaaa agcgcaagaa
     1501 tttggtgtaa gaataaataa gttggatgaa aatccagata cttgtgtaag ttatctagga
     1561 gatgcaatag gaatgactcc tgctagtgtt ggtagttatg gtagttggga tactatagat
     1621 tttattagaa acataaagcc atgtggattt aaagatggaa tagtaacaaa gtatattaaa
     1681 aaagaaaatt ttaatatgta tgaagatgga actaatgtag aagatgacac agatgttatg
     1741 attgaatttc ctaaatttta ttggaaaata cagagttctg ataactacat ggatatattt
     1801 atatctaaaa caaaattaga tgatgattat gaatgtccag cacatctaat tggtagtaca
     1861 gaaaaagaat ttatttatat aggtgcatat ttagcatcat tggaaaataa taacttaaga
     1921 agtaaaaaaa atagtagccc agagctgaga gaacgtggac gattctacag tttaatatat
     1981 aaacatgttc ctggatatga ttctttggat tatcaatgtg tattaatgtt acagattcta
     2041 tttgttgtaa tgtttaaatc aataaatttt tttaggttag gagttggtta tagctcttat
     2101 atatcaggtc atggaaagcc agataaaata aacacaggcg gttctaattt caaaggtatg
     2161 atatatggag aacaaaatgg agatgagcaa atgaaatttt taggaataga ggatttatgg
     2221 ggaaattgtc tacaggaggt ggggagatgc acttgtggag ttgaaaatgc ttgtgtatat
     2281 acaagaataa ttacaaggga taaaagaaat atacaatatg gagtaggaag aaaggttggt
     2341 acaatatcag atgtaatagg tggtaacaac actggtttta tggttaaaca gtgtcttcct
     2401 ggaggaggaa atggtcaaga tagtcgatat tatagatgct gggctaattt aagtgaaaac
     2461 catataggat attacacagg gtttgattat aatgaaaatt ttagatatta tggtttattt
     2521 tcatcttatt ttcaaacaga ggtaaaaaaa gcatcaacta gattaatttt tataggagaa
     2581 gacaatcaaa atacactaaa aacaattcaa gaagatgaat tgctgtttgt ggaaaatgaa
     2641 aaagttggag gaatattgta atgatagatg gagtagttgt aagaatactg gcagatagaa
     2701 tattaaataa gggattaaat cctctaaaaa atagagtatt tgaacttgat gatgttacta
     2761 acacagaata taggatagca gttgaaaact acattataga acatagtgag gtagtagaag
     2821 gagcagaact tacaaaatag gtaatgttct ttttttaata caaagcaata ggaggttttc
     2881 atgaatgaag aacttgtgaa agaaaaaata agtacacttg aaacaagagt aaaagaacac
     2941 ggcaaacaaa ttgatagaat cgagattgag caagcaaagt ttgctataca aatacaaaac
     3001 ctgtgtagtg atataaaaaa tctgacagga gtactcaagt ggcttgtagg agttataatt
     3061 acaactttag tagggttctt tatatttgcc atacaaaaag gaatatttta attaattagg
     3121 aggttaaaag atggataatt taataagttt tataccagag cagttactaa ttttagtagc
     3181 tgctctttat gttataggag caggttgcaa gaaatataag caattagata ataaatatat
     3241 tccaattata ttacttgtgt tgggaatcgg attttccatt tggatgttag ggtttagtcc
     3301 taacgcagtc ttacagggaa taatttgttg gggaatatca ataggtataa atcaaactta
     3361 caaacagttg aaggatggtg aaaagtaatg aaattaacaa aaatattatt actcacaaaa
     3421 atttaaaaaa tttcattaaa tagagtatat ttttatagct aataagtata taataatagt
     3481 atagaactcg taaagcattt atatgctcaa atatacggga cattgatttt tgaaggaacc
     3541 cgccaagctt cttttatagc tcaaataggc gggacagata aatttaccca ctaatattaa
     3601 ttggtgggtt ttattatata aaagtatatc tatgaaaagg tgaaaacaat ggttgaagta
     3661 aaagaagaaa aaacatttga tgaacaaata gatattttaa aaagtagagg attaataata
     3721 aatgataaag aagatgctaa atttgtatta agtaatgtca attattatag gtttacagca
     3781 tatcttctaa gttttaagaa tgatgatggc tcatataaag aaggaactac gtttgaagaa
     3841 gtttatgata tatataggtt taataaggaa tttaggatat tattaacaga tttgttaggg
     3901 agcatagaaa tagcatttag gacatacatt gcatatacat tagcaattaa acatggtgct
     3961 tatggatatc tataaaggga gagtttcaaa gatgaaaaat tctatattaa ttttttgaca
     4021 gcattagaga gagaaaaaag taataattca gataagcttt ttattataca ccataaagaa
     4081 aaatatgaag gaaaacttcc catttgggtt gcaacggaaa taatgacttt tggtattttg
     4141 tcaaaattat attcaaacat gttgccagaa gatactagat acataaaaaa taatttgtgt
     4201 agagtgaata ctttattagt taaatcttgg ttacaatcat taacacaggt cagaaatcaa
     4261 tgcgctcatt atggtagaat atataataat aatttccgta ttataacaat aaaaaacgaa
     4321 tataaaaagt ataacttgga taataaaaag atattttctt atatacttgc tatgaagcat
     4381 ttgactatgg ataaattaat ttggaatagt ttttttataa aacttcaaaa gttaattaat
     4441 gattataata attctataga cttaaagctt attggttttc ctaataattg gatagagata
     4501 ttggctaaat aaaatagtta ctttaagaag tttataaaca cttactatat gtaagtgttt
     4561 ttttattgaa aagaaggagg aaaataaata atgaaaatat gtattacagt aggacacagt
     4621 attttaaaaa gtggtgcatg cacttctgct gatggggtag ttaacgaata ccaatacaat
     4681 aaatttcttg caccagtatt agcagataca tttagaaaag aaggtcataa ggcagatgta
     4741 ataatatgcc ctgaaaagca gtttaaaact aaagcagaag aaaagactta taaaatacct
     4801 agagttaata gtggaggata tgatttactt atagaactac atctaaatgc aagtgatggt
     4861 caaggaaaag gttcagaagt tctatattat agtaataaag gtttagaata tgcaactaga
     4921 atatgtaata agctaggtac agtatttaga aatagaagag ctaaattaga taaaggatta
     4981 tatatcttaa atagttcaaa gcctacagca gtattaattg aaagtttctt ctgtgataat
     5041 aaagaagatt atgagaaagc taagaaacta ggtcatgaag gtattgctaa gttaattgta
     5101 gaaggtgtat taaataaaaa tataaatagt gagggagtta aacagatgta caaacataca
     5161 attgtttatg atggagaagt tgacaaaatc cctgcaactg tagttggttg gggttataat
     5221 gatgggaaaa tactgatatg tgatataaaa gattacgtac caggtcagac gcaaaatctt
     5281 tatgtcattg gtggtgcagc atgtgagaag atagggacta ttactaaaga acattataca
     5341 atgataaaag gtaatgatag atttgataca ctttacaaag cattagattt tatcaataga
     5401 tagatttaat tgatagaaat atattgaaat tagaaaaaat tacacataac taaaggtggt
     5461 aggttaaact tatcaccttt tttatatatt ttcattacct aagttatacc tgtaaatgtg
     5521 tatttaaaat acattcttaa taatataaaa tttaatcaaa aatgaacaat tcctcaattg
     5581 ataatttact attatttata tattagaatt attgctaaaa atgtaaaaaa tatgttaaaa
     5641 tccataaaaa acaataaaat catattattg ttaaaagggg gattatcaaa tgaaatgggc
     5701 tagtaaaaaa atatttattt tatcattatc aagtataaca atactgtctt ccgttggaag
     5761 agtaaacgcg ttagaaatta aaatgaatag taatttaaac aaacagatag aaaagagaac
     5821 tactatacct gatttagata cattgacaat ggtacaagct tttccagatg caaattttag
     5881 agcatatctt tgtgatattc tatctataaa agatgaaaat gctcttatat ctaaagactc
     5941 taaggataaa attgaagtta tagataattt gggtttatat gaaaaatcaa taaaagattt
     6001 atccggagtt aattatttcg ttaatttaaa aaggttatat tgtagtaata ataacttgaa
     6061 aacattagat gtaagtaata atccagaact cgagatttta agttgttatg gaaataactt
     6121 ggaaacatta gatgtaagta ataatctaaa acttagagat ttacattttg gaggtaataa
     6181 tattaaaata ttggatataa gtaaaaacaa aaaacttgaa atactatatt gttatgataa
     6241 tagtttaaaa acattagatg taagcaataa tccagaactt acacatttaa gttgttttgg
     6301 aaataacttg gaaacattag atgtaagtaa taatccagaa cttaaggaat tatattgttt
     6361 tcgtaatgac ttgaaaacat tagatgtaag tgataatcca gaacttatac gtttagactg
     6421 tagtgataat atgcttaaaa cattagatgt aagtgataat ccagaactta tacatttaga
     6481 ctgtagtgat aatatgctta aaacattaga tgtaagtaaa agtccagaac ttaaagaatt
     6541 atattgttat cgtaatgatt tgaaatcatt agatgtaagt aataatcaag gacttaaaga
     6601 attatattgt tatgataata acttaaaaac attagatgta agtaataatt tagaactcac
     6661 acatttaagt tgtggtggta atatgcttaa aacattagat gtaagtagga acaaaaatct
     6721 cataattctt gattgggaaa accagagaaa agaatcatca ggaggaaatt cttatactcc
     6781 agttccaata gtaacaaaga caaattattt ggtaggctct gatagatatg atacttcagt
     6841 aaaaataagt aaagaaggtt gggataatgt agataatgtg gtacttataa acagctctag
     6901 tataccagat gcattatcag ctacaccatt tgcgaaagct aaaaatgcac caatactact
     6961 tactcaaact aatagtttga atcaattaac agaaaatgaa ataagtagat taggcgcaaa
     7021 aaatatatat ataataggcg gatttaactc tgtggatgca agcattgaaa gttatttaaa
     7081 agataaaggc ttaaatataa taagaatatc tgggaatgac agatatgata cttcaataaa
     7141 actagcaaaa gagctaaata aagaaaataa attatctaag ttggttttag taaatggaga
     7201 aaaaggtctt gctgatgcag taagtatggg ggcaatatca gctaaagaaa aaatgccaat
     7261 acttcttact aatgaaattg atgatatgaa agctatagaa gaattgattg ataataaaga
     7321 tatatcaaaa tcatatgtag ttggtggaga tagtttattt aatgaagaca ttggagataa
     7381 acttccatat gtaattagaa tatcaggaga agatagagca gaaactaact caaaagtaat
     7441 agattatttt tatgataata acgttttaaa taatttatat attgcaaaaa atggagaaaa
     7501 tagagaagat gatttagttg acgctttatc agtaggagta cttgcaggta agacagaatc
     7561 tccagtacta ttagttggag atggtataaa agatagtcaa aagagtttaa taaataaaag
     7621 agaatttaga aatataactc aaattggtgg aaatggaaat gaaaaagcct ttagtgaaat
     7681 agaaaattta gtgaagtaga tagattaaga ggtagtaact ggaaatagtt actacctctt
     7741 tttttattca ttttcatctt tttcaataat tatttttcca tcctcaaaag taacaagaac
     7801 atccctttca tcaggagaaa tattcatctc tttaatccat gacattggaa gtatcaatct
     7861 aggtgtaaaa gaacctttgc cacttttatt gaatgcaacc ctaagttttc tttgttccaa
     7921 taatatcatt cctttctact attaataaat gccagcaata ataaagtaaa taccaaagca
     7981 ataagagctt taaaagtaaa acttattatt atattaataa tatttaaaac tatagtgata
     8041 ccaagaataa tcatacaaat tctataaaat ttattattca tattaaatta gaaaatgtgt
     8101 tataatatta gaaagaagct acctagaagg aaggtatttt cctcctagga ttgaaactta
     8161 taattcttta atcaatctta gtattgcgag tactagatta attaaaacta atgtgaaaga
     8221 gaaagcattt ttaattttat taaatttgtt ttctctttta ttttttcttc tttctaatct
     8281 ccccactttc tttgtcctcc cttctaagtt ttttagtttc ttacctcctt tctatattta
     8341 tattatatca tcacgtgacg tgaaagtcaa taaatattcc atatttttca ataaagttat
     8401 attgacaaac tatatttttt tacaaataaa aataagggtg gcaaaaccaa acttgccacc
     8461 cttttgccac cgaaaataaa taatagaata aatttaaatt aaacataata aaacataaac
     8521 aacaaaactg accttaataa aacattgaaa taccaacact tgtaaaaaat aacaacaata
     8581 aacaaataaa atagaataat attacaactt aagtaaataa taattttgaa taataattta
     8641 cggaggtatt catatgttta aacacgataa agcattatta aaagaagtaa aagtagaaag
     8701 accaaatcct caatatgctg ttttaatgca agaacagttg ggtggtgcaa atggagagtt
     8761 aaaagcagct atgcaatact tgtcccaaag ttttagaata aaagaccctc aaataaaaga
     8821 tttatttctt gatatagcag cagaagagct tagccatatg gaaatggtag cacaaactat
     8881 aaatttatta aatggacatg atgttgatta taattcagtt aatacaggcg aaatagaaac
     8941 acatgtatta actggtctat caccagtctt aataaactca tcaggagctc cttggactgc
     9001 taattacgtt acagttacag gggatttagt agctgactta ctttcaaata tagcttctga
     9061 acaaagagct aaggtagttt atgagtactt gtatagacaa atagatgata aatatgtaaa
     9121 agaaacaata gatttcttac ttaatagaga agaagcacat aatgctctat ttagagatgc
     9181 tttaaataaa gttaaagata caggttcaaa tagagatttt ggagttacag aagattctaa
     9241 attatatttc gatttgtcta ctccagggcc aaatcacgat actaagatag atattaatcc
     9301 tccttctttt gaaaaacctt taaaaaaata atttgatatt ttttatgttg ttttgtttta
     9361 gtagttataa aaattattta ttagaagaat taattatatg aaaaattaaa tatgtatagt
     9421 taaaaatggg tatacactta ttataagtgt atatctattt gtttattgca aaacatataa
     9481 taaaataaca aatgaggtgg tattttggac agtaatagaa aacctaataa tctaataaat
     9541 gaaaaatctc catatctttt acagcatgct tataatccta taaactggta tagttggaat
     9601 gatgaggctt ttaaaaaagc aaaagaagag gataaaccaa tatttttaag tgtaggatat
     9661 tcaacatgtc attggtgcca tgttatggaa aaagaatctt ttgaagatga agaagttgct
     9721 gaaataatga atagaaactt tgtggcaata aaagtagata aagaggaaag acctgatgta
     9781 gatagcgtgt atatgacagt ttgccaagct atgacaggta gtggtggatg gccaatgacc
     9841 ataataatga caccagataa aaaacctttt tttgcgggaa catatttccc aaaatattca
     9901 aggtataata gacctggagt tatagattta ttagagaatg tatctgaaaa atggaataca
     9961 agcagggata tattaataaa atctggagat gaaataatag aagctcttaa ggatgatttt
    10021 ggtgtaaaaa atactgaggg tgatttatca aaagagatgt tgagttcatc agttagagta
    10081 tttaaggcta tttatgatga aaattatggt ggttttggta atgctcctaa atttccaagt
    10141 cctcagaatc ttatgttttt aatgaaatat tatagtatag aaaaagataa agatgtgtta
    10201 aaaatggtgg aaaaaacttt agatggaatg tatagaggag gcctgtttga tcatattgga
    10261 tttgggtttt caagatactc tactgataaa aagtggttgg ctcctcattt tgaaaaaatg
    10321 ctatatgata atgctatgct tactatagct tttttagatg cgtataaaat aacaaagaaa
    10381 gagttgtata aagaaattgc tataaaaact atagattatg tagttagaga gatgaaggat
    10441 aaagaaggtg ggttttattc agcacaggat gctgatagtg aaggtgaaga aggtaaattt
    10501 tatacattta atcctcttga gataattgaa gttttgggtg aagaagatgg aactttcttt
    10561 aataattatt ttgatataac tagtagtgga aattttgaag gcaaaagtat tcctaatctc
    10621 ataaaaaata aagaatatga gagacataat gaaaagatag ctgaccttag taaaaaggta
    10681 tttgaatata gaaaagaaag aacatctttg cataaagatg acaagatact tacatcatgg
    10741 aatgcattga tgatagttgc tttaacaaag gcatatagta ctttaaaaaa tgacatatat
    10801 ttagagtatt caaataagtg tcttaacttt ataaataata atcttgtaaa tgagtcaggt
    10861 agattattag ctagatatag agatggaagt tccgactatt tagcatattt agatgattat
    10921 gcttttttaa tctgggcgta tatagagctt tatgagtcaa catttaatat gaaatattta
    10981 gaaaaggctt taaatttgaa tgaaagctgt atcaaccttt tttgggatta tgaaaaaagt
    11041 ggattttata tatatggtaa agatagtgaa aatttaatag caagaccaaa agatttgtat
    11101 gatggagcta ttccatcagg aaattctgtt caattataca atcttataag acttgcgaaa
    11161 attactggag ataatagact tgaagaaatg tcatataaac aattaaaact gtatgtggat
    11221 aatgtaaaga gttctccaac tggctacagc ttttatatgc tttcattgat gtttgaactt
    11281 tattctacta aggaaattat atgtattttt aaggaagatt ctgatttaat tgcctttaaa
    11341 gaattgataa gtgaaaattt tataccaaat gcaacttttt tagcaaagaa atacaatgaa
    11401 gaaaacacta ttataggttt tttaaataac tatagattga aggatgataa gattagttat
    11461 tacgtgtgtc aaagtaatag ttgtagtcaa ccaattaatg atttacaaaa attaaaagat
    11521 atgattttag gagtagaata atcataatag tgaaaaagga gatgctttaa aataaaagtc
    11581 taatcatctc ctttttttgt acaaaaaaca gaaaaagagt tcctgagaac acctaatcta
    11641 tgtaatgatt gttttgttaa aaaataaaaa tgacattata gttatataaa atattgtact
    11701 ataaaaaagt agtattatag atgcgatagt attaatcgtt tttatttact ctctttcttc
    11761 tttttgcaaa ttcttcaagt gcttcagatt taattttatc tatttcttca tattccataa
    11821 aattagcatc ttcttcaaag ccatcttgtt cttcaccatt aaaacgatac atttttttta
    11881 gtaaactcca taaggtctct aattcatctt tggtaaattc gtgaaataca tctgccataa
    11941 agtttattcc agtcctacta catgttacca taaccttttt tcctaagtct gtaactttaa
    12001 cattaattgc tctcttatca tgaggacttg gaattacatc aacatatcca tttttttcaa
    12061 gattagcaac tagtctattt atattttgtt tacttgtacc catttttctt gcaatattat
    12121 ttaatgttgt ttcttcctct ggtaaatgaa gaattgataa aatagtcata tactgtcttg
    12181 aagtaagtat accaaaatat ttatctcctt ctatttgtat tttgtttgta agggtaaaaa
    12241 gagttgcata tgtttgattc attaaaaaga gttcttttag ttcatttgaa taatccatga
    12301 aatatatctc ctttattttt agatgttgtt ttgtcgaaaa cagaatatca tgaattaatt
    12361 ctaatgtcaa tatggtgtca aaatatatta aagtattact tttttatgta actattaatt
    12421 aattaaatta ttgtataatt ttatgtaaaa acttatatgt accaatgata atttctatga
    12481 ggaattaata attcttaaat tttttagaaa gattttgact tgaaaatact tgtaatataa
    12541 tatataaatt aatatactga ataaaatcaa tctggaggga taattatgaa gtgtacaaac
    12601 tttactttca aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat
    12661 ataaaaaaac caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg
    12721 tatgagactt ttgccaaggt acttactaaa aatggttata tagtatatat caatgaccat
    12781 agaggtcatg gaaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa
    12841 ggatttagat gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat
    12901 gaagatttgc caatttatct atttggacac agcatgggtt catttgcaag tcaaagatat
    12961 ataatggatt acagtaataa tttatctggt cttatactat gtggttcaaa tggaaagcaa
    13021 ggaatcattc taaatcttgc tcatctgata atcaatcgtg agattaaaaa gtatggaaga
    13081 cgttctaaaa gtaataaaat aaataatttg atatttggtg gcgaaataat aagaagaaat
    13141 gaaaagacta agtttgactg gttaagtaga gataaagaac aagttgaaaa atatataaat
    13201 gacccatttt gtggagtagt gtgtagttgt ggcttttttt atgatttagt tcaagggtta
    13261 aaagaaattg aggataaaga aaacttaaaa aaagtgccac ttgatatacc tatttacata
    13321 atatctggag ataaagaccc cataggaaaa aatggtaaag gagtcttaag actaagggat
    13381 agatatataa aattaggagt aaaagatgta acttgcaaac tttacaaaga tggaaggcat
    13441 gaattgttga atgaaataaa tagagaagaa gtttttgagg atataatttg ttggttaaat
    13501 aataaaatag aaatattgta ataaaaaaat ataatgatat atttgcttta aatttattta
    13561 tatataatat ttgaaaagtg gattatgtat atgcaaagta gaaaagagag agcccagaag
    13621 tgattttttg ctataatgcc atttattgaa taggtgggtg ttattataaa taaattatta
    13681 acactcagta ttattatcat tgtatatgga atttatttta tttaaaattc cttgaaagtt
    13741 taaatatagt aagttgtagt aaagagaaaa tctaaatatt taaatgctat ttaatgtgaa
    13801 tctattaatt aggttatact taaacttaat taatagattt tatatttcaa gccaatgaga
    13861 tagggtttta gattaaaata taaccaatgg ttaaattaca gacattttgt taactttcct
    13921 tcgatttact agtaaatatt tttttaatat acttaaatta caacagatgt actagggagg
    13981 tatatatgca aattaaaata ggtaaagtca tacagcgtct tagaaaagaa cgaaatttaa
    14041 ctcaagaaca attagcaaag tttataggtg tgtcaacacc agcagtatct aagtgggaga
    14101 gtggaaattc ttacccagat atagaattat tgcctttatt agcagacttt tttaatgtgt
    14161 ctattgataa gcttttaaat tacaagattg atttaagtga agaagaagta atgaaaattt
    14221 ataaagaatt agaatcaggc tttgctagaa ttgaaattga tttatcaaca gaagaaccaa
    14281 aagaagaatt tagacaagac ttagaatctg taaaaaagct ttccaatatg tatatagaga
    14341 agtatcctaa aagctattta ttgaagctaa ggatatgttc gctgtatcag atgtattcat
    14401 ataaatttgg taagagtgaa cttaatgaca gagtaaaaga aacaacgaat attcttgaag
    14461 atatagtgag aaatacagat gatatccaaa taaaagaaac agctcttatt atattatcaa
    14521 acgcatattg tatgttggag gattatgaaa aggcagagtt gtatttaaat atgatacata
    14581 agtcaatagg ggatacaagt gttaatttag ctatgatata tttaaagcaa aatagacttg
    14641 aagaagcaga aatattgctt caaaataaat tgtttagtaa tgtatttaat ataagcatgg
    14701 attgtaaggg aataattaat gtatacaaaa atcaatacaa ggaattaaag aaaaaattgg
    14761 agaatagaaa ttttaataaa aatgcaattg agagagaaat ggagtatata aaaaataagt
    14821 tgctggggta tgcaaatctt tctttagaaa taaaaaaaat gctcagtgaa gataaaggtg
    14881 cattttttag tatgtatatg gattatatgg aattatctct aattttttta ttttttaaca
    14941 tgaaagaaga agcaaaaaaa gccttatata atctaaaaga aatattagaa aaatatccta
    15001 tacatgaaaa tttggatgta agtcaaatga gattttttga taaggtagag tctaaaaatt
    15061 tatatacttt taatatatat acaaacttgt taattgtact taatgatgat agttataatg
    15121 agcttagaga agaaccaatt tttaaggatg taattgaaaa gattttagat atggaaaaaa
    15181 tgttgaaaaa taaagaataa gtaaaaattt aatataggac ttaaagatta atcaaattaa
    15241 aagaaagtgt ttcaaaataa aaatttgaat cacctttttt gtctgtctta aacagttatt
    15301 tagaaaaata gaggtttcaa aatagaaatc tgaattatct gtattattac acaaaaatag
    15361 aaacctaaat ttataaaaat agaataaagt ttgttatata aaatgtaatt ttgtctataa
    15421 aatctcacct gttttctaac tttaagcgtg aaaaagagaa tgtatcatga attttttagt
    15481 tcatgatgca tccttctttg tatgaaatcc aatatatctt tattatttaa acaatggaaa
    15541 agagtcttat ttctattttg agacatctct tttatttaat tatgaagttt tatacagttc
    15601 aataaatagg aattatattt agaaactcat gacattattt cttagatagt gattaaattt
    15661 atcatattcc attttagata acttttctgg gtaagacatt acaatcaggt tatgcccatt
    15721 aaatttttca tggtatggtg gatgaatgtg ttcataatta tttgtcttat attttactct
    15781 ttcatagaac gccttgcgat aaattgaaat ctcatcaatt ggaaggtcaa tttctaaaat
    15841 aatagttttt cctttcttat ttaaaagctc taacgctctt tgtccatatc tattattgcg
    15901 catttctgga aaaatgcaga aatgttctac atagataaag tcatctgttt cccaacataa
    15961 aattatgccg acaaactggt ttttatcata aattaaattg aattgatatt ctttattttt
    16021 caaaatttct tcttgcaaag aagattttct ttgttcatga aatgggaaac taattttata
    16081 taattccata gccttgtaat ataatttatt ttcactggtt cttaaccgtt caaatttcac
    16141 tttactcctc ctaatcacga aaattccaat ttatttatga gttcatttta ccatagtcag
    16201 tatttgcata caatgtgttt taagttgagt gtcatttctt tcttttattt atgcttatat
    16261 ctcatagact aagagataat tgcttaagac ataactaaaa actattatat taagctttga
    16321 taatctaaaa acagtatatt tatggacttt tctaggtcta aaagtaatct taataatttt
    16381 agaagttaca aaaaagttac ataaatttgg aaagctataa tatacatata taatataagg
    16441 ttaaaactgt aatgaataat attaatttaa aatttgaaca tggaaggaga ttattatgga
    16501 gaaaagagaa aagaggataa taatatcatc tttattaagt gtatcaattt taatgggttt
    16561 ggtaagtata tattctatat taaataaaga agacataatt ttaactgtta agggtcaaga
    16621 gcaaaaagta tcttccttta agaaaacagt tgaggaactt ttggatgaac agggtataaa
    16681 gtataattct gaagataaga ttaatccaag tttagataca gaactaaaag atgatatgaa
    16741 aataaaagtt gttaaggtaa ctaaaagtaa aaaagaagag attgaaaaaa ttccatttga
    16801 tacaaagcat gtaaatgata gtaatttgtt aaaaggaaaa tctaaagttt atcaagaagg
    16861 tcaagaagga gaaaaaaaac tagtctataa tttaacttac catgatggaa agttagtcaa
    16921 aaaagtctta tcaaaagaag taatatctaa ggagccaact acaaaaatta taaaatatgg
    16981 aactaaagaa aaagtactaa tagcatcaag aggagcaaat ataagaggag gcaaacatat
    17041 gaaagtggtt gccactgcat atgcaggaga tacaataaca tctactggta caactccaag
    17101 atggggtgtt attgcagttg acccacgtgt aataccatat ggaacaaaag tatatatacc
    17161 taaactgggt atgacttttg tagcagaaga ttgtggaggt gcaattaaag gtaatagaat
    17221 agacattttt atgaacagtg aaggaaaagc ttcaaattgg ggaaggaaaa gtatagatat
    17281 atatctacat taaattagtg aatagaataa ctttacttga aggacaaaaa agtataaaat
    17341 atgaattaac taaactgtta gattattctt aaaacataaa aatatatcta aggggggata
    17401 agatgaaaat agtctgctta ggagacagtt taacgtatgg atttggagta tcacgaagta
    17461 attcatggac aaacattgta aataaagaga ctcgattaga aatagtaaat aagggtataa
    17521 atggtgatac aacaagtggt atgttagcta ggtttaatga agatgttgta aaaaactctc
    17581 ctgatatagt ttttataatg ggtggcacca atgattttat tgctggtgcg ggaaatgaaa
    17641 ttattaattc taatataatg gctatggtgc atcagtcata ttcaaaaaat attataccta
    17701 taataggtat accattaaaa ccagatattc caaatgttag ggaagattgg agttgtttta
    17761 ctgattttaa tatagtatct caaaacttag aatcatatag ttattggata aaaaaatttt
    17821 gtatgacatt taatactaat ttcgtagatt tttattcaga atataataaa aatatggaac
    17881 tagaaggata taaaaaatta tattttgatg ggttacatcc aaccaaagag ggtcatagaa
    17941 taatggcaga catttttatt aattcaatta ataagtatat agaagagtta tagattacat
    18001 taaaatgctt gtttatgcgt cagttgcaat aaatacatac agtagagagg ggtttattat
    18061 ggattatact aaaacttatg aagagtggat aaagggttca tattttgatg aagatacaaa
    18121 gctagagttg gagaatataa aaaataatga aaaagaaata gaggatagat tctataagga
    18181 tttagaattt gggactgcgg gtcttagagg aataattgaa gcaggaacga atagaataaa
    18241 taagtacaca gttagaagag ctacttttgg attggcaaac tatatattag aaaacacaac
    18301 aaaagaagaa acaagtagag gagtagtaat agctcatgat aatagacaca agtctagaca
    18361 attctgtata gaatctgcaa atactttggc agcatgtggt ataaaagcat atatatttga
    18421 tagtttgaga actactccag aattatcttt tgctgttaga agcttaaatg caatcgctgg
    18481 tatagtcata acagcaagcc ataatcctcc agaatataat ggatataagg tatactggga
    18541 agatggagca caagttatgc cagagattgc aaatgcaata acagagaaag taaatagtat
    18601 acatgattat agtacaatac caaccttaac ggaagctaat aaaaatttgg tagttttgtt
    18661 ggacgaatca caagatacta aatttataga agtagtaaaa agccaaatta taagaaaaga
    18721 tttagttaaa aatgtaggta aaagctttaa gatagtttat acacctcttt gtggaacagg
    18781 gaatgttcca ataagaagag cattgaaaga agttggtttt gaaaatataa ttgtagttcc
    18841 agaagaagaa aatccagacc caaattttgc aggacttgat tatccaaatc cagaagagaa
    18901 aaaagcttta aatagaggta tattactcgc aaaagaaaaa ggagcagacc ttgtaatagc
    18961 aactgaccca gattgtgata gagtaggtgt agcagtaaaa actactacag gtgagtatgc
    19021 tttacttaca ggaaatcaaa ttggtggtat gctgacacat tatataatag aaggattaaa
    19081 agaaaataac aaattaaaag aaaatcctac aataataaaa accatagtta catcagaatt
    19141 tggagcagat atagctaaag caaataatgt tgatgtatta aatgttctta caggttttaa
    19201 atttattggt gaaaaaataa aattatttga acaaaataaa aatagaagtt atgtatttgg
    19261 atatgaagaa agttatggtt acttagttgg gactcatgct agagataaag atggtgtagt
    19321 ttcttcattg ttaatatctg aaatggcagc attttactat tcaaaaggta tgagtttata
    19381 tgatggttta atagaacttt ataaaaagta tggatttttc aaagaacaga ctatatcttt
    19441 gactttaaaa ggtatagaag gtgtagaaaa gataaaagaa ataatatctt attttagaga
    19501 aaatcaaatt gattatataa atagtattaa agtagttgat aaaaaagatt ataaaaatgg
    19561 tatagataat cttcctaaat ctgatgtttt aaaatacttt ttggaagatg aatcctgggt
    19621 agctataaga ccatctggaa cagaaccaaa acttaaattt tacatagctg taaaaggagc
    19681 tagtgatatt gaagcagata aaaagattca aggtttgaaa aagtatatag atgacatggt
    19741 cgaaaaatta aaataaaata ttattatttt ataagataat tttgtaataa tatttattta
    19801 gctgaaaatt ataatttact ttcattgagt agaatatgga aatgttagta gaattgttat
    19861 tttcagcttt tatttgtgtt atttttactt aaataattat attatagaat gaatctgagt
    19921 ataattaata ttgtataatg aaattaattt gatagaaaag gggatattta tgtttggtat
    19981 agataagaaa ttgattgaat atataattgc aaaatccaat tacagtgaat taagttcaaa
    20041 gtcagcagaa gtatctttct tcttaatgtt gtccatattt ccttttttaa tatttacaat
    20101 aagttctata gcttatatac cgattcttca tttaaataag tatatagctt tgtttagaaa
    20161 tatgatgcca gaaggtgctt ttgctgtgtt atcttcaatc atagtttctg caatagacaa
    20221 tagaaacctg aaatttttag cagtcagttt tgtacttact atgtggacat tttcaagagc
    20281 agtaaaagct cttattaaag gaatgaatag agcctataaa gttaaagaaa ctaggtcttt
    20341 ttttaaaatt ttatctatat catttttatt tacaataatg cttttggtcc ttattttttt
    20401 atctatgata tttttagttt acggggaaaa aataggttat tttattttta atcttgtagg
    20461 attggatgag atatttataa aaatatggga tattctaaga tacactgtag gtataataac
    20521 aataatagtg atatttacac ttttatataa atacactcca aataaaaagt taactattaa
    20581 agaatctgcg ccaggtgcta tttttgctac ttttgcttgg tttttagtat catttttata
    20641 ttcttattat acaaattact atgctaacta tgaagtaatc tatggaagta tagctgaaat
    20701 aattgtactt atgacctgga tgtattttag tagttggtct atcgttattg gctatgaggt
    20761 aaattcaaga ttgtatttta gaaagataag acatgaaatg ttaaagtaaa gtatttaaaa
    20821 taaaagagag tatttagtat tataaatgta attgaattaa taaagaaata ttgacattta
    20881 agttaaaaag tctagaattt ttctgggctt tttttatttt tagtataact tgacaaattt
    20941 tatttaatgg gatagtttta atttataatc atctttaaaa agatgactat tgataagtaa
    21001 gagataaaat tggtaagtat caaaaaatat actaaataaa aattatagtc ctaatgagtt
    21061 aatttctgta ttttcaaata atgcagtatt tagtcatgtg tttttatatg tgcctgtatt
    21121 tctaattacc aatatattag aataagctac tgtaattagg tttagaaaaa ag
//
LOCUS       DAFKMX010000055        21160 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFKMX010000055 DAFKMX010000000
VERSION     DAFKMX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.055"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            165..686
                     /locus_tag="LZB08_001626"
     CDS             165..686
                     /locus_tag="LZB08_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315132.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKRDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            720..1496
                     /locus_tag="LZB08_001627"
     CDS             720..1496
                     /locus_tag="LZB08_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7315133.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSANM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1838..2791
                     /locus_tag="LZB08_001628"
     CDS             1838..2791
                     /locus_tag="LZB08_001628"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBM7315134.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2809..3828
                     /locus_tag="LZB08_001629"
     CDS             2809..3828
                     /locus_tag="LZB08_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBM7315135.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3859..4110
                     /locus_tag="LZB08_001630"
     CDS             3859..4110
                     /locus_tag="LZB08_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315136.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4392..6197
                     /gene="lepA"
                     /locus_tag="LZB08_001631"
     CDS             4392..6197
                     /gene="lepA"
                     /locus_tag="LZB08_001631"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBM7315137.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6346..7683
                     /locus_tag="LZB08_001632"
     CDS             6346..7683
                     /locus_tag="LZB08_001632"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBM7315138.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7916..9229
                     /locus_tag="LZB08_001633"
     CDS             7916..9229
                     /locus_tag="LZB08_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7315139.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9401..10576
                     /locus_tag="LZB08_001634"
     CDS             9401..10576
                     /locus_tag="LZB08_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBM7315140.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10679..11698
                     /gene="hrcA"
                     /locus_tag="LZB08_001635"
     CDS             10679..11698
                     /gene="hrcA"
                     /locus_tag="LZB08_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBM7315141.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11728..12348
                     /gene="grpE"
                     /locus_tag="LZB08_001636"
     CDS             11728..12348
                     /gene="grpE"
                     /locus_tag="LZB08_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBM7315142.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12440..14287
                     /gene="dnaK"
                     /locus_tag="LZB08_001637"
     CDS             12440..14287
                     /gene="dnaK"
                     /locus_tag="LZB08_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBM7315143.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14498..15652
                     /gene="dnaJ"
                     /locus_tag="LZB08_001638"
     CDS             14498..15652
                     /gene="dnaJ"
                     /locus_tag="LZB08_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBM7315144.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16618..17553
                     /locus_tag="LZB08_001639"
     CDS             16618..17553
                     /locus_tag="LZB08_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBM7315145.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17589..18962
                     /locus_tag="LZB08_001640"
     CDS             17589..18962
                     /locus_tag="LZB08_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315146.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19150..19917)
                     /locus_tag="LZB08_001641"
     CDS             complement(19150..19917)
                     /locus_tag="LZB08_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315147.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(19947..21056)
                     /gene="ugpC"
                     /locus_tag="LZB08_001642"
     CDS             complement(19947..21056)
                     /gene="ugpC"
                     /locus_tag="LZB08_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBM7315148.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8262 a         2388 c         3741 g         6769 t
ORIGIN      
        1 tataactaat atgttagaag gttatatgta attaatgtca gtgtttatat agaatagcat
       61 ttgtagcatt tattatagtt gtaattacta tttatatcat agacttgaat agtctaaaaa
      121 aatagactat aatattttta tagactaaag aaagggctga taagatggac atagcaaaac
      181 taattttaaa tcctgcaagg cttcgtattt tacaatatat tcgacttcat ggcagtgttc
      241 gtacatctga tattgtaaag tatttaaatg atattcctcg tgcaacagtt tatcaccatg
      301 taaaaatttt agaagagaac aatatgattg aagtaattaa agaaaatcgt gtgcgtggta
      361 ctattgaaaa ggtttatgca ctaaaggaat atacaacatc tatggaagga gagaccttcg
      421 ttgcactttc tacagcattt tatgtaggtc tgatgcagga aatgaatgaa tactttagca
      481 gaaaagagca agaccataaa agggataatg tatttttctc ttcagcatta ctatatatga
      541 gtgataatga atatgaaaat cttcttaaaa gtatagctga tttattgaaa ccatatatag
      601 aacaaaaacc taaatcaaat ctaaaactta gaaaactttc aattatatct tctccacctg
      661 tagctgataa tgataaaaca aaataacatg caacccattt ttataaaagg aggttatata
      721 tgaaatttca tgagtttgga aataaaaaca gaccacatat tatgttgatt catgggggag
      781 ggaatgcatg gtggaactat ttgcgacaag cagaagtttt gtctaacaaa tatcatgtga
      841 tacttcctac attggatggt catggagaag aatatgaaac aacatacatt tctacagagg
      901 atacagcaga taagttgata tcatatatag atgaaaattg cagtggacat cttttcgctt
      961 tgtgtggggt atcacttggt ggtcaaattg tcatggaact tttatctcga aaacctaata
     1021 ttactaaaaa agcaattatt gatggtagta tttgctaccc aagacctctt atggcaaggt
     1081 tttgtattgc tggtattcga ttttttggag gacttttgtt tagtgagaag gcttgtcgct
     1141 ttcaaattgc tatgatgcca aaattgttac ctgaaaatat gcaatatcca gatgaaataa
     1201 aagcatatta tatgacagat atgccacatg taagaaaaga aacattctat aatatgtatc
     1261 gcacttacat gatgaactat acacttaaag agaatgttaa aaaaaccaca gcacaagtaa
     1321 tgtactggta tggagaaaaa gaaatgaaat gtgttaaaaa atctgcaaac atgtttcagt
     1381 cttatgtgcc ctcttgtgag atttatgaag ctaagagcta taatcatggg tatttatcac
     1441 tttatcttcc aaatgaatgg ttggaaattg cagaaccatt tttccaaaag gaataaaggt
     1501 gttaggtgat tattgtataa ttattgctgt tttttaatat agtaaataaa tcaggtataa
     1561 atataaaaaa ttattttagt taatatatta gtgaaagctt aataggaaaa ttataaatag
     1621 attaaaggta gcgcctatat aaatagttgc tacctttttc tgtttgtaaa taaatgatag
     1681 ataagaaggt ttactgacaa atcactgact aaacccaatt ttcttacatt gaaagcctta
     1741 attttacttg ttttaaataa atttacaatt tcttgttata aaatactaaa ttttgttaac
     1801 aattataatt agcatttaat ttatactgga ggtttatatg attagtgtaa gaacagattt
     1861 agctttagaa gctagtgaaa tgtgtgaaaa gtctcaagaa ggaagtagta ttcctggtgt
     1921 aaaaatagaa actaaagaat tagaaaattg cattgtaact aaagttgaag taatagacag
     1981 gcaaggcgca gagataatga ataaagatat aggaaagtat ataactctgg aaagtaatct
     2041 tatgaagttt gatgatgatg agtcaagaga agaaatgata agttatttaa aagaggagtt
     2101 agtagatata tttggccaag acaaaaataa aaaaaccctg gtaattggtc ttggaaatag
     2161 aaatattact tctgatgcgt taggaccaaa gagtgtatca aaaacacttg taactagaca
     2221 tctttttaaa aattataata aagactatga tgatgatttc actgaggtat cagctttaag
     2281 cccaggagtt atgggtgtta ctggaataga aactagtgaa atagtaaaat cattggttga
     2341 gaaggttaag ccagatagag tagtagcaat agatgcattg gcttcaagaa aaatggagag
     2401 agtaaactct actatacaga tatcaacagc aggaatatca cctggtggag gtgttggcaa
     2461 tactagaaaa tctctaacta aagaaacttt aggcgtggat gttattgcaa taggcgttcc
     2521 aacagtggtt gatgcagcaa ctcttacaat agatgtctta gatatggcaa tagacaattt
     2581 aatagctcaa tcagaagaaa cagaaagttt ttatgaaatg ttgaaaaaac ttaaagaaga
     2641 agaaaaatat catctaataa aagattcgtt agacccatat gacaagaact taatagtaac
     2701 accaaaggat atagatgata caatcgaaaa tctatctata ataataagtg aaggcttaaa
     2761 taggtcttta catccaggta gattggtata aaataaggag gagcaattat gtttaaaaaa
     2821 tgcattaaag tagtaacctt gacttttata ttagtatgca ttttaccagg aaaatcatta
     2881 gctttgaatc aagatgattt tttaaagttt ttagtaaatt catcttatcc agaagctaaa
     2941 gtcgaaggaa atgatacaga aaataaaaag aataataaaa ataaagaaac aagtaaggaa
     3001 aataaggaag aaagtaaaga agaaaacact aaatctaaag atgcaagtaa agttgataat
     3061 aaaaaagaga gtgaaaaaga atatataaaa ctttatgttg gtaaagaaaa tgttccagat
     3121 attgagtcaa aaaattcaga cacaactgaa actaacacaa catctagttc agattataag
     3181 gatgatttaa gagtaaccaa agaaaatcca agaattttga tatatcatac tcatggatgt
     3241 gagacttatt caaattcacc tgatggaaat tatcattcaa gagataagaa aaattcagta
     3301 atggaagtag gaagtgcatt aactagtgct ttagatagca aaggatgggg tgtagtccat
     3361 actactaaat accatgacta tccatcttat aataactctt atgcaagtag cttaaaaaca
     3421 atacaaagta tattacctaa atataactca gtggatatag cgatagactt acatagagat
     3481 gctagggatt taactaatcc agccactaag gaaaaagacc atttaaaata tacaactatg
     3541 ataaatggag agagagtatc aaagttcttc tttgttgtag gaggtaaaaa tacaaataga
     3601 aaacaactta gagctttagc agaagatata actgcttttg ctgagaaaaa gtatcctggt
     3661 ctagtatctc caatagtaga aaaagactat gctagattca atcagtttgc agttaagaat
     3721 catatgttag tagaaatagg gaataacgca acaagtgtag aagaatctaa ggctactaca
     3781 aagtatttag ccgagatttt ggatgaatat tttaaacaaa aaaattagag ctttaagtta
     3841 taattaggag catgctaaat gagccgatta gaaaaaaata tagaaagtaa aagtaaacta
     3901 agaaaataca agtatattat gagaatctta tttttgatta ctatgattat aggtacatca
     3961 gtttgtgtat ttatagtaga cagtaatgca aaaagtatgc taggaatgaa acaatatgat
     4021 tttccaagta ttaaatttga tataacaaaa tttgattttg acataccaaa ttttgatata
     4081 catgaaatca taaaaaaatt taataagtag taataagtga ataaggttta ttgattaaaa
     4141 taagccttat tttagttata tatttttaat gtataaaata aaaattcatt aactgtattt
     4201 tataggattt actttaagtt tacgatatta tataatattt agtgttagac ttgataatcc
     4261 acatataatg tggataattt tgtttagtgc caattagttt atattcaaaa attttaaatt
     4321 tggtatataa agagttttta gataatgtaa aataataact ataagaataa tcaagaggag
     4381 ggaattttaa ggtggacaat aaacaaagta gaactagaaa ttttagtata attgcacata
     4441 tagaccatgg aaagtctacc ttagctgata gattaataca acaaactggg cttgttagtg
     4501 aaagagacat gaaaagtcaa ttacttgaca atatggacct tgaaagagaa agaggaatca
     4561 ctataaagct tcaaaacata agattaatgt ataaagcgaa ggatggaaat gaatattatt
     4621 taaatcttat agatacacct ggacatgtag actttaacta tgaggtatca agaagtttag
     4681 ctgcatgtga aggagcacta ttagtagttg atgcagctca aggtgttgag gcacaaactt
     4741 tagccaatgt ttatcttgct atagaccaag atttagaaat attaccaata ataaataaaa
     4801 tagatttacc aagtgcaaga ccagaggaag taaaaaatga aatagaagat ttaatagggc
     4861 ttgattcaag tgaagctcct cttatttcag ctaaaactgg tttaaacata gaagatgttt
     4921 tagaagatat tgtaaaaaat gttcctccac ctaaaggaga taatgaagct ccattaaagg
     4981 cattaatatt cgattcttac tatgatgctt acaaaggtgt tgtagcttat gttagagtat
     5041 ttgaaggaac tgttaaaaag ggtatgacaa taaaaatgat gaataccaat aaaaagtttg
     5101 aggtaactga agttggtgtc atggctccag gtcaaactga acttagtgaa ttatcagcag
     5161 gtgatgttgg ttatattgct gctagtataa aagatataag aagttgtcgt gttggtgata
     5221 caataacaga ttcaaataat cctacagaag aacctttacc aggatacaaa aaagctactc
     5281 caatggtata ttgtggtata tatccaggag aaggagaaaa gtatgagaat gttagagatg
     5341 ctcttgaaaa gcttcaagta aatgatgctg cacttgaata tgaagctgag acttcagcag
     5401 cactaggatt tggttttaga tgtggtttct taggtctttt acatatggag ataatgcaag
     5461 aaagacttga gagagaattt aaccttgata taattactac agctccatct gttatatata
     5521 gagttacaaa aatggatgga gaggttgtaa tgatacaaaa tcctgcaaac ttaccagaac
     5581 catctgagat aaagatgata gaagagccta tagtaaaagg agatataata gttcctaaag
     5641 actatgtggg tgttgttatg gagctttgcc aggaaagacg tggaaatatg ttaaatatgg
     5701 aatatataga tgagagaaga gtaatgcttc actatgacct tcctctcaat gaagttgttt
     5761 acgatttctt tgatgcttta aaatcgagaa ctagaggtta tggttctctt gattatgagg
     5821 ttaaaggtta tgtagcatct acacttgtaa aacttgatat attaatcaat aaagagcaag
     5881 tggatgcact tagttttatt gtccatgaga cacgtgcttt ccctagagga aaggcaatgt
     5941 gtgagaaatt aaaaggagaa attccaagac atcagtttgc tataccaata caagctgctg
     6001 ttggtaataa ggttattgca agagaaacaa taagtgctct aagaaaagat gttcttgcta
     6061 aatgttatgg tggagatata tctcgtaaga agaaacttct tgaaaaacaa aaagaaggta
     6121 agaagcgtat gagacaaatt ggttctgttg aagttcctca aaaagcattt atgtctgtgc
     6181 ttaaattaga tgagtaaatc cttataaatt tagataaaga tatttaaaag ctagagtgat
     6241 tttagaatta tttctaaatt agtctagctt ttttatggta tattttaagt aaactcatag
     6301 agaatataag taagaatatt aaagaagaat aaaaaggtga tatttatgat taatatagaa
     6361 attcctaaaa aagtagacta tataattaaa gaattagaaa agaatggtta tgaagcttat
     6421 atagttggag gatgtgttag agattgtcta ttagaaagag ttccaaacga ttgggatata
     6481 acaactagtg ctagaccaga agtggtagta gaattatttg aaaaaacaat tccaactggt
     6541 atacaacatg ggactgtaac agttatgatt gaacatgaac catttgaagt gacgacttat
     6601 agaattgatg gaaattatag tgatggtaga catccagatt caatagaatt tacaaataac
     6661 atagttaagg acttgtcaag aagagatttt actatcaatt caattgcata caattcaaag
     6721 acaggattag tcgacccatt taatggctat gaagatatac aaaataagta tatacgttgt
     6781 gttggaaatc cagtagatag atttgaagaa gatgcactta gaatgcttag agcagttaga
     6841 ttttcagcac aactcaattt taaaatagct gaaggtacta agcaaagtat tcataaaaaa
     6901 gcagatttga taaaaaatgt ttctattgaa agaatccaga cagagtttaa taaaatatta
     6961 gtatctgatt catctaaatt aaacttatta aaatcaacag gtttgttgaa atttataatt
     7021 cctgaaatat gtgaattaga agatgttact caacacaatc catatcacat ctatgatgta
     7081 caaaaacata ctttaatagc tacagaagta attgaagatg aactgtattt aaaacttaca
     7141 atgttgtttc atgatttagg taaaaaagta actaaaacaa ctgataaaaa tggtgttgac
     7201 catttttata ctcatagtag agaatctgta aagattgcaa agaagatttt gaaaagactt
     7261 aagtatgata attatactat taataaagtt ttaatattaa tacaatatca tgattataga
     7321 atagaaccta aaaggaagat aattaaaaag ttattaaata aacttgaaga tgttgaatta
     7381 tttgaagatt taataaaagt taattgggct gatacacttg caaaaaatcc aaagtatgca
     7441 aaacaaaaga tattaaatct tattgagtgt gaaaaagaat ttaaacatat aataaatcaa
     7501 aaggagtgct ttaatcttaa agatttagct attaatggaa aagatttgat atctataggt
     7561 ataaaaccag gtaaagatat aggatatatt ttaaataaga tgcttgaaat agtcataaat
     7621 aatccagagt taaatgaaaa agaaatacta aaagaaaaag cattaaatat atatacattt
     7681 tagattcaaa tataaaaaat ttaattactt taaaaaacat agaataaaat acttatttag
     7741 ataaaataaa taatttttag agaatatttt agaagttacg attgtatgat ttttaaatgc
     7801 ataggcattt actctaggac tatagactaa tataaaaaaa aactttggga atactaaaac
     7861 cataaattta taaaattaat attttatata ctgtaaaaca gtgaggaggg acagtatgca
     7921 tactcaacta caaaagacta ttggtctttc agcagctctt tctactgttg taggtatggt
     7981 tataggttcg ggagtttttt ttaagccaca agcaatatac actacaacta atggagctcc
     8041 aggtctaggt attatagcgt ggcttttagg tggttttata acaataactg ctgggcttac
     8101 agctacagaa atttcagctg ctataccaaa aactggtggt atgatgattt atatcgaaga
     8161 aatatatgga gaaaagttag gttttttaac tggatggatg caaactgtat tatttttccc
     8221 aggtacatca gcggcattag gagtaatatt tgctcaacaa gcatctgaac ttttaggaat
     8281 gagcccaaat aatatggcaa atgtacttcc tatagcaatt ggagttatac tatttttagc
     8341 gttattaaat ataataggtt catccttagg aggaaaagtt cagacagtag ccactattgg
     8401 aaagatgata cctttaattt taataatagt ctttggattt attaaaggtc aaagtagtga
     8461 agtattaaat ccatttgtag gagatggtgt aaatgcatca aatgcacttg gacaagtatt
     8521 aatagctact ttgtttgcat atgatggttg gataaatgtt ggagctatat caggagaaat
     8581 gaagtctcca gagaaagatt taccaagagc aatagttggt ggtttatcat tagttatggc
     8641 agtttatatt ataataaata tagcttatct ttgggtagta ccagcaagtg aattagcaac
     8701 agttacttcc ccagctacat tagtagcaac aagattgttt ggaaatatag gtggaaaagt
     8761 aataactgtg ggtatattaa tatcagtatt tgggacattg aatggatatt tattaacagg
     8821 ttcaaggata ccttatacat tagctgaaat gggaacttta ccagcatcta aaactctttt
     8881 aaaagtaaat tctggaggtt ctcctgtaaa ttcaatacta cttataactg tgttagcctg
     8941 tgtatatgca ttatctggtc aatttaatct tttgacagac ttaacaatat tttcaatatg
     9001 ggtattttat gtattgactt ttataggtgt tatgaggctt agaagacaaa agccagattt
     9061 acacagacca tataaagtcc ctttatatcc aataatacca attatagcta tattaggtgg
     9121 actgtttgta ataataaatc aaatattgac ttcaacagtc atatctttag gcggaatatt
     9181 tataacacta ttaggtttac cagtttatta ttatatgaaa aaaagataac tacttaattt
     9241 aagttagata aatttgggta aaggtgaaaa ttaactttta tccaaatttt ttatactgtt
     9301 atacaatata agtattatat attgcttgat gtgctatttt gtttgattta taatttagta
     9361 attgatgttt taagataatt taagaaatga ggagtgaaag atgctaggac tttatgtaca
     9421 tattccattt tgtgtgaaaa aatgtaagta ttgtgatttt aattcttata aaatggacat
     9481 agattcaaaa aaaagatata tagaagactt aaaaatagaa atggaattat atagtaataa
     9541 attatataaa gataataaat ataaaaataa agaatgctgt agtctaaata aaaatgacaa
     9601 aataacgagt atatttgtag ggggaggtac acctagtata ctaacctctg atgaaataag
     9661 agaagtattt ataagtataa aagaaatgtt tgatatagat gagaatgcag aaataactat
     9721 agagtgtaat cctgggacat taacattaga aaaattaaaa actatgaaag agattggcat
     9781 aaatagactt agtatagggc tacaagctat tcaagaaaag catttgaact ttataggaag
     9841 aatacatact tatgaagaat ttgagaaaaa ctataaagat gcattaagtg taggttttaa
     9901 aaatattaat atagatttaa tgtacagtct tccaaatcaa actctatgtg attggaagga
     9961 aactcttgaa aaagttgtac acttaaatcc aacacatata tcagcttatt cccttatact
    10021 agaagaagga actgaactat acaatatgta tgaaagtaat aaatttgaac ttatagatga
    10081 aaatgtagat atagaaatgt atgagtatac aataaactac ttaaagtcaa aaggatataa
    10141 tcaatatgaa atatcaaact actcaaaaga aggctataat tgtgagcata atattttata
    10201 ttgggaatgt gaacattata tagggattgg agcaggagct tctggctata ttaatgagaa
    10261 cagatacaat aatgtagaat ctttagaaga ttatcattta agtttggtaa aaagagaaaa
    10321 accaatacaa gaaaatgaaa ttctttctga gaaagatatg atagaagaaa agatatttat
    10381 ggggcttaga atgaataaag gcataaaatt tgaagatttt aaaaaaaaat ttggaataga
    10441 ttttagagag aagtataata agcaaataga aatgctatta gctagaaagc ttataaatca
    10501 gagctttgaa ggtatacaat taactcaaaa aggaagagaa atatctaata gtgtatttat
    10561 agaatttatg gaataatttt taaggtgttg acaaaataaa tttataatgg tatataatat
    10621 atataatctt tttagcactc gaacttagtg agtgctaaca gctaggaggt ataataatat
    10681 ggaattaaat gaaagaaaat taaatatcct caaagctata gttaaagatt atatagaaac
    10741 agctgaagct ataggttcta gaacaatatc taaaagacat gatttaggtg ttagtgctgc
    10801 taccataaga aatgaaatgg ctgaccttga agaattaggg tatttaattc agcctcatac
    10861 ttctgcgggt agagttccat ctgaaaaagg ctataagtta tatgtaaact ctcttatgag
    10921 taaaagcgaa ttggatgata atgataagat tcttattgaa caatgtatga atcacaatat
    10981 aaatcatata aaagaattga ttcatgaaac ttctaagcta ttatcgcaac taacaaacta
    11041 tacaactgta gcagttacta agagtttaat aaatcaaagt gttataaagc acatacagtt
    11101 ggttgctatg aatgacaata atattttact tatagttgta acagataaag gggacttaaa
    11161 aaaagctaac cttacaacta atgtatattt agatcaatct aagttaaatt tgatttctga
    11221 taatcttaca aggaagctct taggtaaaag tataacagat ttggatgata atctaattgc
    11281 ttttattaaa tacgaaataa gtgagtattc tgggcttata gatgagcttt taaatgcttt
    11341 gaactcaaat atgaaagaag aagatttttc tctatcatta aatggagcta ctaacatatt
    11401 tagttatcca gaatttaatg atgtattaaa agcaaagtca tttttaaata tgttggaaaa
    11461 aaaggaaaca atagctgaca taattaaatc aaagggaata caaaaagaca atctaaatat
    11521 aataattggt agtgataatg attgtgaact ggcacaggat tgtagtatag ttacagcaac
    11581 ttataatgta gatagagact tggttgggcg aataagcttt ataggcccta ccagaatgga
    11641 ttatgctaga atttactcta taataaatta tatgagttta ttaattaata gaaagtaaag
    11701 aaattttaaa ttgcgaggtg taaagtcttg gataaaaaaa atgagcaaca ggaagtaaga
    11761 gaagaaaatg atacttctat taaccaagaa tcggaaactc aagtagagtt agaagaggaa
    11821 gttgttaatg aagaatgtga aacatcaagt gaaaaaactg atgaaaaaga agttgatgat
    11881 gaaaatgtaa cagatataaa ttctaagtta gcagaaaaaa aattgcagga tgagttggat
    11941 gaattaaatg ataaatatca aagacttcaa gccgaatatg caaactacag aagaagaact
    12001 caacaagaga aagagacaat aggcgtattt gcaaatgaaa aaataataac tgaattgata
    12061 ccagttatag atagtatgga aagagcctta gatgcatgtg aagataagga agatactatg
    12121 tataagggta taagccttgt acataaacaa ttaatagaca ctttagtaaa atttggagta
    12181 gaagaaatag aagcagaatc taaggaattt gaccctaatc tacatctagc agttatgcaa
    12241 gaaagtgtag atggagttga agcaaatcaa atagtaatgg tacttcaaaa aggatataag
    12301 ctaggaacta aagttgtaag accttcaatg gttaaagttt cttgctaagt ataaattaaa
    12361 ggttaaatta tttaacctaa tataaaatat taaaataagt aaaacttaat tatataatta
    12421 ctcgaggagg attttagtca tgggaaaaat aataggaata gatttaggaa caacaaattc
    12481 atgtgttgca gtgttagaag gtggagaagc acaaataata gcaaatagtg aaggtatgag
    12541 aactactcca tcagtagtag catttacaaa agatggtgaa agaatagttg gagaacctgc
    12601 aaaaagacaa gcagttacaa atgcagataa aacaataact tctataaaaa ctcatatggg
    12661 aactgattat aaagtaaata tagatggaaa atcatatact ccacaagaaa tatcagcaat
    12721 aatattacaa aaattaaaat cagatgcaga aagttactta ggacaaacag taacagaagc
    12781 agttataaca gtaccagctt actttacaga tgctcaaaga caagcaacta aagatgctgg
    12841 tagaatagca gggcttgatg ttaagagaat aataaatgag ccaacagcag cagctcttgc
    12901 ttatggtatg gataaattag accaagaaaa gaaaatatta gtatttgact taggtggagg
    12961 aacttttgac gtatctatac ttgaaatagg agatggaact tttgaagttt tagccacagc
    13021 tggtaacaat agattaggtg gagatgactt tgaccaaata gtaatagatt atttagcaga
    13081 ggaatttaaa aaagctgaag gtgtagattt aagaaatgat aaaatggctc ttcaaagatt
    13141 aaaagaagca gcagaaaagg ctaagaaaga attatcatca acaatgagtt caaacataaa
    13201 cttacctttc ataactgcta cagcagaagg accaaaacac ttaaatatag atttatcaag
    13261 agctaaattt gaagaattaa ctagaggttt agttgaaaaa actatggaac caactaagac
    13321 agctttacaa gatgcaggac tttctacagg tgatatagat gatgtattat tagtaggtgg
    13381 ttctacaaga ataccagcag tacaagaagc tgttaagaag tttataggaa aagaacctca
    13441 taaaggtata aacccagatg agtgtgttgc agcaggtgct tctatacaag ctggagtttt
    13501 agcaggggat gttaaagact tattattact agatgttact ccattatctc ttggtataga
    13561 aactatgggt aatgtaatga ctaagataat agaaagaaat actacaatac caactaagaa
    13621 gtctcaaata ttctcaactg ctgctgataa ccaaacagct gttgatatac atgttcttca
    13681 aggtgaaaga agtatggctt atgacaatac aactttagga agattccaat taacagatat
    13741 accaccagca caaagaggaa tacctcaaat agaagttact tttgatatag atgcaaatgg
    13801 tatagttaat gtttcagcta aagatttagg tacaggaaaa gagcaaaaaa taactataac
    13861 atcaaacact aatttatcag aagcagaaat agagcaaaag ataaaagaag cagaaatgaa
    13921 tgcagaagct gacaaacaaa agaaagaaaa aatagaagca ttcaatcaag ctgaatctac
    13981 tatatatcaa actgaaaaaa ctttaaatga acttggagat aaaataagtt caggtgaaaa
    14041 agaggatata gaaaaagcta tagcagactt aaaagctgta aaagataatc aagatgctac
    14101 agcagaagaa cttaagaaag ctacagatga agtaatgact aagttccaaa aagtatctca
    14161 agaaatgtac caaaaagcag ctcaagaaca acaagcggca caaggtgcag aacaagcaca
    14221 agacaatgga cctaaagatg acaatgtagt agatgctgac tttaaagaag tagatgaaga
    14281 taaataagct atataatatg taacactaaa tgataattgg aacatatatg atttgtgaaa
    14341 atcatataga tgttaaagat atatggacaa tatacatcat aaagaatata atatatcata
    14401 gattatataa cttaaagtag aatataagaa ttaatgatag cttgtagttt attattctac
    14461 aagctatcgt tttgtaaaat gaaggtggtg tcaatttttg agtacaaaaa gggattatta
    14521 tgaagtctta ggtatcagta aaggtgcaga agctcaagag ataaaaaaag cttatagaaa
    14581 attagcaatg aagtaccatc cagatagaaa tcctggagat aaagaggcgg aagaaaaatt
    14641 taaagagata aatgaagctt atgaagttct atctgatgat acaaagagaa aaacttatga
    14701 ccagtttgga catgatgggc taaatggtca aggcggtttt ggtggtcaag gaggatttgg
    14761 cggtcaaggc tttggtggtt ttgaagatat gttcggagat atatttggtg acatgtttgg
    14821 aggaagcttc ggaggtggaa gagcaagaag aagagggcct caacgtggtg cagatataag
    14881 acagagtgtt actatatctt ttgaagaagc tgcatttggt aaaaagatgt ctataaaagt
    14941 aaatagaagt gaagaatgtg aagaatgtaa tggaactgga gcaaaaccag gaacatcaaa
    15001 gaaaacttgt tcaacttgta atggaactgg tcaagttaga actgttcaaa gaactccatt
    15061 tggtaatata gcaagttcaa gaccatgtag tgcttgtaat ggaactggag aagtgattga
    15121 atctccatgt agtaaatgtc atggaactgg aaatacaaga aaagttaaga ctatagaagt
    15181 agatatacct gctggtatag atgatggtca aatgataaaa ctttctggtc aaggtgaagt
    15241 aggagaaaaa ggtgctccaa gaggagactt atatattgta gttaatgtta agtcacatcc
    15301 actatttact agagatggaa atgatattta ttttgaaatg ccaattacat ttgttcaagc
    15361 aactttaggt gatgagatag aagtacctac tttggatgga aaagttaaat atagtgtgcc
    15421 agaggggaca caaactggaa ctgtatttag acttaaagag aaaggaatcc ctagaattag
    15481 aggtaattca agaggagacc aatatgttaa ggttgtagtt gaaattccta aaaaattaaa
    15541 cgataagcaa aaagaactat tgagagaatt tgcgaaggag tgtgggtcga acgtccacga
    15601 aaagaagaaa accttcggac aaaagataga agatatgttt aagaaaaagt agttaaagta
    15661 gccaatatat aatcatattt ttgaaatctt agttatttat aagattgtca aaggagatat
    15721 tatgattttt atattggcta tttttgtata aaaaataaat gtgcataaaa taaaactgaa
    15781 tataaataat tttctgaaaa atacttgtcc taaaatatat aatatgctat aatataatca
    15841 aataaaagaa aggggtgatt ccagtgggaa cggatgtggc actgccaaat gatgaagaaa
    15901 actttattgt aatatacaaa ataaaattag atacttttag tgctagatta agtactggaa
    15961 aaactcataa gctgggataa ttattgtaat tacctagact caaactttta gtagtaaaat
    16021 agtaatatta tatagacaag gtatctaatt tttaaatctt ttgtatgaat taaaattttt
    16081 ataaagggta tatctgatta gaatgtaatt agggaaacct agttgtgaat tacttttagt
    16141 atatacatgt gtaaagaaat atgtatatgg tattggttat tgtgctagat gtgtatgtaa
    16201 aggaatatgt gtctagttgg gtatatgtaa agaaatatac ttataatcta tgaaaaattt
    16261 ttagtataaa ttgctaaaac attgagattg ttttttatag aaagttagtt tataccaact
    16321 aaaaatgtgc ttaagatttg tttgatgttt aattataaat aaggttgtag ataaaaaaat
    16381 aagtaaagac aaaggtttaa aatacttgct ttaaatatag tttgttttga acacttgcca
    16441 aattaaaata attaataaaa tagacatctt ctcatagtaa aatatactgt gagaggatgt
    16501 ttttgctttg ttcataaact tttatgaaaa atttgcatag tgtcacattt taatatacta
    16561 tatatataat aaaatataga taaaattaaa tgattcaaaa gagaaaggtg aaaagagatg
    16621 tattatatag gtgtagacat aggaggaaca ggtatacaag caggagtagt tgataactat
    16681 gggaaaataa tttttaggtc agagtgtaaa acagtaattg aaaaaggctt tgaaggaata
    16741 cttaatgaca taaaaataat gatttataag ttgttggaag ataataaact tactatgagt
    16801 gacataaaat ctattggatt tggtgttcca ggttttataa ataaagaagg attagtaact
    16861 tgtgtaaatt taaaatggaa taaaaaagct tttaataaag agttaaaaag aagatttcca
    16921 gatgttgaaa ttcatggaga aaatgatgca acagtagcag cacttggaga agcaaagttt
    16981 ggaagtatga aaggtgctaa tgttggagta ttatatactt taggaactgg tgtaggtgga
    17041 ggaatagtaa ttaatcaaaa agtcttttct ggagcacatg gattgggttc agaaataggt
    17101 catcaaatta taggagaaaa ctattttaat tgtaattgtg gaaataatgg ttgtgtagaa
    17161 actttttgtt cagcaactgc aattataaag tattctcaaa aactgataga agaaggagaa
    17221 aagagtagga ttttagattt ggcagaggga aatttagaaa atgtaaatgc aaagatggtg
    17281 tttgatgcat atagagaaaa tgatttagtg gcaattaaag taataaatag atttaaagag
    17341 tatttagcaa aaacttttgc aaacacaata aattctttag acccagaaat tatatctata
    17401 ggaggaggca tttctaaatc tagcgatata atacttgatg gaatagaaga tttggtaaga
    17461 aaatttgtat tatacaaaac agaagatata gctacaatta catgtgcaac tttaggttca
    17521 gacgcaggaa ttataggagc agctttttta taatgtaaat gaactgctta ttggaggcgt
    17581 gatgatatat gggtaaaaca aatgctagaa gaacgctttt tttgatagca ataggttcag
    17641 gaactatgct gaatcctctt aattcttcta tgatttcact tgctttacat agtatacaaa
    17701 atgattttca tattagtttt acaactgtat cttggattgt ttcagcattt tacctagcta
    17761 gtgctattgc acaaccagtt tcaggtaaga taggagattt aataggtaga aaggtcttat
    17821 ttttgagtgg tttgatgctt gtaattttat cagcatccat agtaccactt gttcaatcat
    17881 tctttatatt gatttttata agagtaattc aagcaattgg aacaagtacc ctatatccat
    17941 ctggagttgc tcttatacaa aataatataa aagaaagaca atcttctgca ttagcagttc
    18001 taaccatttt tgcttcaact acagcaggat taggtccgac acttggagga ttattgttgg
    18061 acttaggagg atggcatgct atatttcttg taaatatacc tgttgttttg gtttctatat
    18121 gcttagggta ttttttattc ccaaaagaag taaaagaaaa gaaatctata aaagaaactt
    18181 taaaaaatgt tttgtcaaga ttagatatta ttggcatact tctattttca attgcaatga
    18241 tttttatatt actatttttg ctatctatga aggagtcttt taacttagaa caacttgtat
    18301 ttggtataat actgatgtgt ctttttattt ggcatgaatt gagaacaaaa ttgccattta
    18361 tagatattaa gctttttgta agtaatccta aattgtcaaa agtttacttg caatttatta
    18421 tattaaattt ctttaattat attttgtttt ttggtcttcc tagttatttt caagatgcct
    18481 tacattatag tgcaaagtcg actggattat ttatgctatt tatgtctggt ataggtatat
    18541 ttatatctcc attaactgga aaatggatag ataaatctgg aactagattt cctcttgtta
    18601 catcatcaat ctttatgttt attagtgcag tacttctttc agtagttttt atccatatac
    18661 ctgtaatagg aaagggtatt atattatcat tagcaggtat tagttatgga gttggtaatg
    18721 tagccctgca atcctctatg tttgaagaaa gtcctcgtga cagtataggg acagcttctg
    18781 gtctgtttca aactagtcgt tatttaggtt ctattttagc aactgtttta cttggaatgg
    18841 tatttgaagt aaatataaca tcagaacaat ttcagatatt aggttatgtt atggtggtct
    18901 taggtacaat aagtttttta ttaaatttta tattaaagaa agaattaaga aaagtagaat
    18961 agtggattta tggatttaat aagtctaata actgataagt ttaaaaatat tgtttgtaaa
    19021 acataactgg tttgatattt attaattcta agttttgaag catagagtac tcgataagta
    19081 tacaatattt tgaattaaaa ttaagcagaa atttgaacta gtctttcaaa tctctgctta
    19141 atttagtagt taaatttttt tatagagaaa ataagatttg acgcttagat aaaaaatcat
    19201 tgcattttca aaattcttta aatcaaaccc aagtatctca ttaatctttt ccaatctata
    19261 tactagcgtg tttctatgta aaaacatact agtagatgtc ttagaaatat ttaaatcatt
    19321 atttataaat tttattcctg tatatagaag ttcttcgtca atagatttaa tcttattaaa
    19381 atctattaat ttattgagta tattatcctc attaattaaa tctaaagttt tatatctaat
    19441 aaggtcaaac aaatttaaaa acttattttc agcgttgtct tttataatat gaggtagtaa
    19501 atcagaacat aactgtaatc taaaatcaaa atcagcttta ctatctaatt caccaagata
    19561 aaaaatataa gtactttgat aagtttcagt ttcaattata ttttgtgtag ttacattaat
    19621 atctaagtta tatttagaat ttactataaa aaaaccattg tgttgtatta tatttatatc
    19681 ttcaaataaa ttataaaaaa tattttttag atattcaaag tctatatgag attggtaaat
    19741 taaataaaag ttgcctttta aattatcgag attattttta tctagaaaat caataacata
    19801 aatagcatta ttgaaagtaa ctaaatggct actagcatct aacttttggt tttcatatgg
    19861 atagatattt atttttttat taagctgtct ttctaaaaaa tcttttaact cttccataaa
    19921 gtctccttaa aaataagatt atattcttat cttatagcta aagtagtttc tttgtcaaat
    19981 atgtgtactc tactattgtc aactgaaagt gttaagtcat caccaacatt aattctgttt
    20041 ttactgtcaa atctaattgt tatcgtattt tcgcctagtt ttaagtgagc ataagtttct
    20101 gctcccataa gttcttttat ttcaattctt gatttaaaac aagtatctgg attagattta
    20161 ataaaagtat cttctacatg aatatcttct ggtctgatac ctataacaac ttcattatta
    20221 atataattgt cttttattaa atcatagtgt ttttctttat tcaatttaag cttaatatgt
    20281 tcattttgtg cataaatttc atcattttct tctattaata ttacatcaat aaaattcatc
    20341 tgtggtgctc caataaatcc tgcaacaaat atattagctg gataatcgta tacatcttgt
    20401 ggagttgcaa tctgctgaac tactccatcc ttcattacta cgattctatc agccatagtc
    20461 atagcttcaa cttggtcgtg tgttacatag ataaatgtag ttccaagctg ttggtgtagc
    20521 ttagttatct ctgttctcat agcagttcta agttttgcat ctaagttgga tagaggttca
    20581 tccattaaga ataccttagg atttctaact attgcacgtc ctaaggcaac cctttgtctt
    20641 tgtcctccag ataatgtttt aggtttttta tttaaaagag gtagtaagtc taatatttta
    20701 gcagcttctt taactttttt gtctatttca tctttaggca attttctaag tttaagtgca
    20761 aatgccatat tttcgtaaac agataagtgg ggatataaag catagttttg aaaaaccata
    20821 gctatatctc tatcttttgg ttctatatta ttgacaagtt tatctccaat gtatagttct
    20881 ccttcagaga tttcttcaag accagcaatc attctaagtg tagtcgattt accacaacca
    20941 gatggtccta ctaaaacgat aaattcttta tctttaatgt ctatatttat attttttact
    21001 gcgttaaatc catttgagta aagttttgat atatttttta aaattacttc tgacataata
    21061 tcctccttga tatttaaatt ttaattatat agtatcaaat ttttttgtta tattaaatgt
    21121 gtaaaatgac taaatgtatt tgtttaatat gtatatttag
//
LOCUS       DAFKMX010000056        20674 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFKMX010000056 DAFKMX010000000
VERSION     DAFKMX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.056"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(377..1768)
                     /locus_tag="LZB08_001643"
     CDS             complement(377..1768)
                     /locus_tag="LZB08_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBM7315149.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLIPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1779..2999)
                     /locus_tag="LZB08_001644"
     CDS             complement(1779..2999)
                     /locus_tag="LZB08_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBM7315150.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(3013..3801)
                     /locus_tag="LZB08_001645"
     CDS             complement(3013..3801)
                     /locus_tag="LZB08_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBM7315151.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3818..5092)
                     /gene="larA"
                     /locus_tag="LZB08_001646"
     CDS             complement(3818..5092)
                     /gene="larA"
                     /locus_tag="LZB08_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBM7315152.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5210..5923)
                     /locus_tag="LZB08_001647"
     CDS             complement(5210..5923)
                     /locus_tag="LZB08_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBM7315153.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            6048..7307
                     /locus_tag="LZB08_001648"
     CDS             6048..7307
                     /locus_tag="LZB08_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBM7315154.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7587..8153
                     /locus_tag="LZB08_001649"
     CDS             7587..8153
                     /locus_tag="LZB08_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7315155.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKFYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8353..9558)
                     /locus_tag="LZB08_001650"
     CDS             complement(8353..9558)
                     /locus_tag="LZB08_001650"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBM7315156.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGHNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SVKKLLKID"
     regulatory      complement(9661..9767)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9859..10227)
                     /locus_tag="LZB08_001651"
     CDS             complement(9859..10227)
                     /locus_tag="LZB08_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBM7315157.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10279..11556)
                     /gene="obgE"
                     /locus_tag="LZB08_001652"
     CDS             complement(10279..11556)
                     /gene="obgE"
                     /locus_tag="LZB08_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBM7315158.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11667..11957)
                     /gene="rpmA"
                     /locus_tag="LZB08_001653"
     CDS             complement(11667..11957)
                     /gene="rpmA"
                     /locus_tag="LZB08_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBM7315159.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11971..12288)
                     /locus_tag="LZB08_001654"
     CDS             complement(11971..12288)
                     /locus_tag="LZB08_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBM7315160.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12303..12614)
                     /gene="rplU"
                     /locus_tag="LZB08_001655"
     CDS             complement(12303..12614)
                     /gene="rplU"
                     /locus_tag="LZB08_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBM7315161.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12715..14136)
                     /locus_tag="LZB08_001656"
     CDS             complement(12715..14136)
                     /locus_tag="LZB08_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBM7315162.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(14154..14855)
                     /locus_tag="LZB08_001657"
     CDS             complement(14154..14855)
                     /locus_tag="LZB08_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBM7315163.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14857..16701)
                     /locus_tag="LZB08_001658"
     CDS             complement(14857..16701)
                     /locus_tag="LZB08_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBM7315164.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEYFNLDIWKEAMEKNNLSIDFYAHRNRNCEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            17020..18213
                     /locus_tag="LZB08_001659"
     CDS             17020..18213
                     /locus_tag="LZB08_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBM7315165.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18425..19243)
                     /locus_tag="LZB08_001660"
                     /pseudo
     CDS             complement(18425..19243)
                     /locus_tag="LZB08_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(19376..20167)
                     /locus_tag="LZB08_001661"
     CDS             complement(19376..20167)
                     /locus_tag="LZB08_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315166.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIDEYKLELERILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20171..20500)
                     /locus_tag="LZB08_001662"
     CDS             complement(20171..20500)
                     /locus_tag="LZB08_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315167.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6539 a         3736 c         2275 g         8124 t
ORIGIN      
        1 ataagttatg atataaaagc tacaaatata tataattcaa tatattataa aaacaataaa
       61 gatttagtaa aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtttttt
      121 aaaataacta tatttataaa aatcatttta ttaactcttt attatataag atacaatagt
      181 cttactttat ttatgtacac atgcttttag gatgacaaca tcaacttaaa tattatgtta
      241 ctttctaaat ttaagtaaat attgccatcc taaaaatttt acaatttagc ttcagattta
      301 aaagtaaagt ttaaaatatt atatttaaat ctaatttaat attttttgtt ttgtaaatat
      361 tattaaatta atatttctaa tatattttcc ctggatttaa aatattttta gggtcaaaag
      421 ctaactttat atttttaatt aacatcatat aagcatctga atttgattca tgtaagtact
      481 cttttttagc atatcctatg ccatgttcac cagaaacttg accactaagt tctctagatt
      541 ttttatacat atagtcaaaa gtttctttta atcttatttt ccatgtatta tcatccatac
      601 catcttttaa aatatatatg tgtagatttc catctcctgc atgaccaaaa ctttttattc
      661 ttatttttaa tttgtcttga agctcgtgag tgtatcttat aaactctgct atcttatctc
      721 ttggaacaac gacatcacat tcatccattt gtgtcgtaga tgcctttata gcttctaaaa
      781 atgcacctct ggctgaccat atagaatcgt ttctttcttg tgtatctgat ataaatacat
      841 ctaatgcacc attttctaaa cataaattag ctactttttc atattctttt tctatatctt
      901 cagtgctatt tccatcaaat gtaagtaaaa gataagcatc tgatgtatta tctggaaact
      961 ttttacctaa aaattcttct gctgccaata ttacatctct ttccataaat tctatagccg
     1021 ttggtattga ttttgatttt attattttag gaacagtttc tattgccatt gataaatctg
     1081 gaaatggtat aagcaaactt atacttttct ttggtaaagg aatcaatttt aatatagctt
     1141 tagtaactat tcctaaagta ccttctgagc ccactaataa atcctttata ctatatccag
     1201 aactattttt aactaccttt ccacctacat ttataatttc tccatttggt agtacaactt
     1261 caagacctct tacataatcc cttgtaacgc catattttac tgctctcata ccacctgcat
     1321 tagtgtttat attacctgca attgtagcac ttttttcacc tgggtcaggt gggtaaaata
     1381 aatctctatc ttgaacatat tgacctattg tcatcaataa aacacctggt tctactgtca
     1441 atgttaaatt ttcataatca acttcaagta ttttattcat tttacataga ttaatcatta
     1501 ttccaccatt tatggcaaca gcagcaccta caagaccagt accttgacct cttggtgtga
     1561 ctggtatatt atttttatat gcgtatttca ttattttaga tacttgttca gtttcaagta
     1621 catttactaa aacttcaggg tacttctcaa tccctccaag ttcatcatgt gaaaaatctt
     1681 catttatatc acttccaact aatacatttt cttctccaca agtatctatt aaaaaatcta
     1741 tatctttttt atcgattaat ttatacattt ctatccccct atttagctgc tgatgccata
     1801 tatttttgat tattatcttt gttattttct attttttcaa tcaatttagg aataatatca
     1861 taaatatcac ctattaaagc taaatgtgct acatcaagta taggcgcttt atcatcttta
     1921 tttattgcta ctatatagtc agcaccctgc atccctgcaa caaattgaac tgctccagat
     1981 acaccacaag ttattatcaa tttaggtttt acagttctac cactaagacc tatttgtttt
     2041 ttagcatcta tccaacctgc ttctattaca ggtcttgttc ctgccacttg agcgtttagc
     2101 ttatcagcta atttatatat catctccata tcttcttgtt tttttattgc tctacttgcc
     2161 acaactatca cttcagcttc ttccagacca acttctttat ttttttcttt tacactcagt
     2221 accttaattt tagattttag ttcttttttt tctactttac ataaagttat ctttcctgta
     2281 gtattctcta ttttttctgg tgctgaaaat attttatatc ttacagtggc aaattgtggt
     2341 ctattattag gtgtgtttat atgagccatt atatttccac caaatgctgg tcttatttgg
     2401 tctaaatcag tatttgattg aatatcaagt attgtacaat ctgctgtaag acctgttcta
     2461 aatctagcgg caagtcttgg agctaatgac ctacctaatg tagttccacc aactaatact
     2521 atcgttggtt ttattttatc tataaagttt tctattgctt ttgaatatgg ttctatccta
     2581 aaatctttaa attcatcttc atcatataca aatacttcat ccaccccata agaaatcaaa
     2641 ttactgcact tatcttttat gtcttttcct acaaatacac aatacacagg ttgttttatt
     2701 tttgatgcca tctcacgagc tttacctata agctcgtacg ttacaggatg tatatttcca
     2761 ttatgatgtt caacatatac tgcaattccc ctccattcat ctttattaat ttgtacttta
     2821 ctactctcta taaattcaaa tgcgcctttt ggtccacttt tgacacacaa cttgcacatc
     2881 ttacagccag cacttatttc tactatacct gacttttctt ctatagcccc aaaaggacat
     2941 atttctaaaa ttctctttaa gtcagttatt ttatcttgat ttacaactat ctttgacatt
     3001 tattactccc ccttacacca actttaattc ctctaattta tttacaagtg caaaacttag
     3061 ttcatctgca tttcccctta caatattttt atctgtattt ggctttggag gaaatattct
     3121 tacaacttga gtaggagaac cattaagtcc atagttattt tcatctttat cttcaaagtc
     3181 atctaagcta tatacaggta tttctctatc tttagtatta agttttaatt taaatgaagg
     3241 tagtcttggt tgaaaaatgt ctttttctac agttattaaa catggatatt caatctcaca
     3301 tacttgtaaa tcatttggca tatctatttc aacaactata ctttcatctt taacttcaat
     3361 taattttgta acgttagtaa catgaggtat atctaaaaat tcagctactt caggtcccac
     3421 ctgtgctgta tcaccatcag tagtttgttt tccacaaatt attaggtcta cctttcccat
     3481 cttcttaata ccttgtgata tggtatagga agtagccaat acatctgctc ctccaaatct
     3541 cctatcactt attaatgctc cttcatcagc tcccatcata aaactttctt caattacctt
     3601 ctttgcctga ggaggaccca tgctaagtac ttttagattc gctttcttat cttcctttat
     3661 tctaagagct gtttctaaag catataggtc atagggattc atttttgaat ctactccatc
     3721 tctttttagt actcctgtag tttcatcaac ttctactgaa gtcgttccag gaacttgttt
     3781 aatacatgtc aaaatttcca taaagtacac tcctttttta tttaactata actgatactc
     3841 catcaggtat aactactact ttgctctcct taccaactat actattcgcc atttctaaag
     3901 cttctttaaa gttaaatgca tgtttcatat gcattttttt tatcatttca ggattacact
     3961 tatctgttac cattatcaca gtatatttag ataatattct cgctagtatc tgaaattccc
     4021 attggtctgg aatcgtatct actctatcga cacttctaat tttatctaaa agttgttttg
     4081 gagaatctgc atttgctaca ttttcataaa aagattgacc tccatgacca tcattacatg
     4141 ctgaaatcat tattattact ccaccgtctt tacaagtagc ttcagctgct gtcattcctt
     4201 taacagattg ataaatattt tggtctagtg gatatccacc atttgtagtt acaacaatat
     4261 cagattttat agaatttact tttgataact ctgttacaaa tctacatcct ttagtatgtg
     4321 ctttttcgac atcaccagca aaggaagcaa ttattttttt atctttatct attacaacat
     4381 tcaatatgaa tgctaatttt gcaactttag ctgcatatac catatcctca tgaataggat
     4441 tattattcaa ttttccagtt cttgaattat cactatctat aaagtcacta cagtgattgt
     4501 acattatagt ctttgcagat gcgattcctg gaagtacgct ttttctgcca cctgaaaaac
     4561 ctgcaaagaa atgaggttct ataaaacctt ctgctatcaa aagttctgcc tcatatgcta
     4621 gtttgtttat atataaatct ccacctgaag gcaatatccc tactttacac atagatttat
     4681 catcagtaga tatatgcata actatatctt cattatctac tatttcttct cccattttat
     4741 aaattagctc ttcccttgta cttggcctat gaaatccagt tgccactata atcttcacat
     4801 caatttctgg attaacattc ctaatccttc ttaatataat tggcatagtt attctacttg
     4861 gtacaggtct tgtatggtca cttgtaatta tgactacatt ctttttcccc ttaaccagtt
     4921 cttccagaga attagagcct ataggattat tcatagattc ttcaactatc tcttcttgag
     4981 tcttatgttc tatataatta tccgattttg actccaaaac accgagaaaa cgttcatcag
     5041 gtatttctaa aatcattcct tgttttgaat acggtacttt taactttacc atttttaatc
     5101 tcccattata ataaactata aaattggtat gtggtcatac cagttttaca tttatatgat
     5161 agtaccatta tctaccattt gcaagaataa tttattttat aacaaacttt taattttcat
     5221 tttttctata atttttactt ataaaatcca tatgttcctt tgctttctca catgcaagtt
     5281 ctacatcatt ttctacaact gcattgtata tagcttcatg ctgtttaagc aatatatttt
     5341 cattaaaagg ttctgcgatt attttttctc ttgcatcttc tataaatcca tcaaataatt
     5401 ttgatgctgt cataaaaaga ctttgaatta aataattttt acttaatgtt gctagtttac
     5461 tgtgaaattt ttggtctaat aacaccagta ctcgattatt atctttttta ttagtttcta
     5521 ccctcatttc atctattata tgtttcaatt ctataacttc agaagaagtt attctctttg
     5581 aagcaagttt tgcaatctca atttctagga ttattctaag ctctaatata tcttcaagtg
     5641 taccattatt aagtttaaaa atcatagata aaggttctat taaagttttt tctatatttg
     5701 tacatataaa atttccttca ccctgtctgc tttctatcac tcccatagtc tctaataccc
     5761 taatagcttc ccttatggaa gttctactca cattcatttg ttcagacagt tctctttctg
     5821 atagtaattt atcccccttt tttaactctc catttagtat tttatattgt atttgctcaa
     5881 ttacttgttc ataaactttt ttactagcta tactattaaa cattttttcc tccaatatga
     5941 taatcgttgt tgtaatatat atctattata tatcttaaac cattacattc atatattttt
     6001 attactttta atataatatc ttataactca aatttacagg tgatattatg aaaaaaaata
     6061 aaattattgc tttttttgct ccatctatca tagttggagt tctagtttta ggtattatcc
     6121 tatacccaaa tgaagctata aaatctgcaa gagaaggctt ttctatatgg tctaatgttt
     6181 tagttccatc actactacca tttataattg gagcaaactt aattgttgat ttaaaaatag
     6241 tggatattat agggtttatc atcaatccca taactagatt tgtatttaat gtttcaggaa
     6301 agagcgcttt agcttttgct atatctacag tatctggtta tccagtagga gtaagtttag
     6361 cttctgaatt taggagtaat ggacaaattt ctaagtatga agcacaaaga cttgtgtctt
     6421 tttgttctac atcaggacct ttattcataa taggttcggt tggtacaggt atgttccaaa
     6481 attcaaactt aggttattta atgattctct gccattatct aggaactata ttggttggtc
     6541 ttttctttag aaattatggt aatgaaaatc ttccaaagac aaaattggat ttaaaaacaa
     6601 atataaaaaa tgtaatcaac attagaaatt caagtgggaa tggttttttt gttttatttg
     6661 gaaatgctgt atttaatgga gtaaacacat tgcttacagt tggtggattt gtaattgttt
     6721 tttcagtcgt atttaaaata ctatctcttt ttaatgtaat ctcattaata gcatcagtta
     6781 tatatttgcc tctttcattt ttaggtgtta gtaaagaact ctgtcaagca tttgtaagtg
     6841 gtctatttga aattactata ggatgcaata aagtatctag tataacaagt tctccagaaa
     6901 ttttaagagc atctttagct agttttttga taggatttag tggattatcc atcctagctc
     6961 aatgttgtac atttctatca aaaacggaca tagatttaaa attatacata ctaagtaaat
     7021 ttgcgcatgg agttttagct gctatcttta tatttatact ttatccaatt gcaaactctg
     7081 ctgtgttggt ttctagcttt gctgatacat acaacgttat atataataat ttaatctggt
     7141 tttattatct tagctattat gatacaattc ttcaaattgt agtaataatt tatctaatct
     7201 cagccgtatt tattgcaaaa aaacatatga aaactttatc tacaaatggg aaactaatgc
     7261 gatataaaaa tacttttttc actaagaaat tttcacgaaa aaaataaaac taaaaataaa
     7321 actaataaaa cttaactaaa tacttccatt tttttattat atttgtcata attttaataa
     7381 cctttttaat tagtttaaca gcatagttat ttcatcacaa tttaataatt ttattagaaa
     7441 tatttttcaa aaaattctcc ttaatcacat atatcacact tgtataagtt tactgggaaa
     7501 tttttccatt atataatatt tcatttatat tatataataa ataccgacct aaacttacca
     7561 aatcaaacta agaggagtgg ttgaaaatgg caacaagacc tatagaaata gacgaatata
     7621 aaaagataat ggaactatta catactggct ttacttatag tgaaaatggc gttgaaaaaa
     7681 gatttagaaa aaatcctaaa gttgcattgg cactaatgtt agaagctaat ttaggtctaa
     7741 gaatatcaga tattttaaga ttaaagatag gtaattttaa aggtaatatg ttggaaataa
     7801 atgaaaaaaa gacgggcaaa ttacagtata gaccagttaa taaaaatata attgagtcca
     7861 taaatgaaca tgcaagaaag tataatctta aatcaaatga ctacttagta aatataaaga
     7921 caaaggctat tcaaaaacag ttgaggataa tttgtaaaca cttaaatctg tataatataa
     7981 gtacacatag ctttagaaag ttctatgcta ctactcaatt tgaaaagagt aacaataatt
     8041 tagagttggt taaagaactt ttaaatcaca gctctgtagc cactacacag cgatatataa
     8101 gggtaactca gcaagctatt aatgaagcta gtgaaaattt ctttattggt taattataac
     8161 gtatacattt ttctattatc ttattaattc accagatatt aaaaaagagt gtctcaaaat
     8221 gaacttttta gttcatgaga cactcttttt ttataaaatc aaatatattt ttattatttc
     8281 aacactggaa aaaggcttat ctctctattt tgagacaccc tattaaatta tcataattct
     8341 tgcataaaca tattaatcta tcttcaataa ttttttaact gattcactgt aattatttct
     8401 ttttacaatt tccaaaactc tatcaatctc atgttctgac tttgcactta caaaaatcgc
     8461 caattcttca tcatctataa tatcataact atctacatat ttatctaaac attctaaaat
     8521 tccatcttta ttattatgcc caccaaatct aactactgca ctacactcat cttctataag
     8581 tccacttaaa tccaatttcc cttttgaaaa agactttata tttgaactcc tattatctat
     8641 tatatctata atatctgcat aaacagcact tcctgttgca cctcttccag ctcccttacc
     8701 tacaaaagaa agttcaccta ctgaatcacc tactaaaata actacgttga actcattatc
     8761 tatctttgaa agcatattat tattgtccac taatgctggt ctaacaaatc cacttacttc
     8821 tcctgattca tacttacttc tagcaaccaa ttttatctta caatttaatt tttttgcata
     8881 ttctatatct ttttcatcta ttgaagttat tccttctaat aataaatctt tccaatatac
     8941 tatttttcca taagataaag tagacaatat agataacttt ctagcactat catatcctaa
     9001 aacatctgca tctggattaa cttctgcaaa tcccaactct tgtgcctgtt ttaaagcgac
     9061 atcatacggt aaattttcat catacatctt tgataatata aaattacaag tcccatttaa
     9121 aatagcgttt atgctcttta tattgttgcc ttccagtgat tctatcaatg gttttaaaac
     9181 tggaatacca cctgcaacag atgcttcaaa cttgatgcta acattattta agttagctaa
     9241 ttctataagg tcactacctt tttcagccag taaatcttta ttagctgtca ctacatgtat
     9301 ttttttattt aacgctcttg ttacataatc aaaagctgga tttaatcctc caattacttc
     9361 aactagtata tcaatatcct tgtcaaatat atcttctata tcagtagtta tcttatcaaa
     9421 atacttctta tacttgtgtt tttctttatt tttaactaaa attccaacaa cttctatatc
     9481 tctttttgaa gtattatttt ctattaaatc aattaatcca cttcctactg ttccataccc
     9541 taatattgct atcttcactg taacataccc ccatcatttt gattatatta aattatttat
     9601 atatataaat ataccaataa aaaaagcttg agtataaact caagccatat gacgggttta
     9661 tactcatctt tcagcataaa tgctgcagga tttagcacca atgtataatt aaatatactg
     9721 gttgccgggc ttcacagggc cagtccctct gccactcttg ataagattgc taatttaaat
     9781 ttatgtttaa tagtataata caatttcttc caattgtcac tattttttta acttatttcc
     9841 ctaaaaggat ataaactatt atctaacagc tcttttagtt ggtttaccct tttttgtaac
     9901 attattattt ttagtctttg ctttcgcctg ttcatgtcct ggaaaaagta ttttaggctc
     9961 ttcaatattc ctcttgtaaa tagtaaattt aaaaccaata gcctgcacaa attcagctct
    10021 taaagcttca catactgcat tagcagtttc tttagtatca agacctgcat tttctaatat
    10081 agtaactttt actaattctc tagttcttaa cataccatct aattgctcta agaaactctc
    10141 tgtaacacct tctttaccaa tttgtgtagt aggctttaaa gtatttgcca aagatcttaa
    10201 atacgccctt tgttttcctt ttaacatatt ctcacctcta taattctata cttaaattat
    10261 ctataatcta tatttaaatt aattgtaaaa ctcaaattct aattcataaa ttttaaccac
    10321 atctccatct tctattccca tttctctaag cctatcaaaa acaccttgac tctccatagc
    10381 tttttggaaa tattgaaggg attccatatc atcaaaattt acagaataca ttattcttct
    10441 aagtgcttta cctgtaacta catacacacc atcttcaata tctatactta actcttcttc
    10501 agtaccaata tcaagttctg gtctatacat ttcttcttct gaaactagct ccacatcttc
    10561 tacttctttt agcattttag aaacataggc tattacttca tctatgcctt gacgagttgc
    10621 tgctgacatt ttaaatactg tatatcctct gccttctaat tcactcttaa acttctcaaa
    10681 cttactctca tcttctaata tatcaaattt atttgcaact acaacttgtg gtctctttga
    10741 aagcttttca ttgtacaatt taagctcatc atttatcttg tcaaaatctt caatagggtc
    10801 tcttccctct aatccagata tatctacaat atgtatcaat acctttgttc tctcaacatg
    10861 tcttaagaac tcatgaccta atcctatgcc ttcagaagca ccctctataa ttccaggtat
    10921 atctgcaagt acaaaactat ctccaaactt agtttgaaca acacctagat ttggtgtaag
    10981 tgttgtaaaa tgatagtttg caattttggg tttagcttta gttacaactg atagaaatgt
    11041 tgatttacct acatttggaa atcctaaaag accaacatct gctatcattt taagttcaag
    11101 agttatccat ctttcttctc catctgtacc agattttgca aaagctggag cttgtcttac
    11161 tgcatttgca aagtgttggt tacctttacc acctatacca ccttttgcaa ctatagctgt
    11221 atctccttct ttttttaaat ctgctaatac aagacctgta gcctcatctc ttattactgt
    11281 acctgctggt acttttaata ctaaattttc tccattttta ccagctcttt ttcctttgct
    11341 tccgtcgcca ccattttgag cttgatattt tttctggtac ttaaagtcca ttagagttct
    11401 aagccccaaa tcaacttcaa atatgatgct tgctccacga ccaccatctc caccgtctgg
    11461 accaccagct ggaacgtatt tttctcttct aaatgctacc gaaccatttc caccatttcc
    11521 agccttaaca aatattcttg ccttatctat aaacaaattc ttcacctact taattaaaaa
    11581 cttttaatta gcattaccaa aaataccatt ataaattcat cagtattata agaaaaagga
    11641 gtgcctcaac actcctttta tgtatattac tcagctattg ctactggata tatactaact
    11701 ttttttctct tcttgtcttt tctttcgaat ttaacagtac catcaactaa tgcaaataaa
    11761 gtatcatcac taccttttcc aacattagtt cctggatgta ttttagttcc tctttgtcta
    11821 actattatac ttccagcagt tactaattga ccatcaaatc tcttaacacc taatctttta
    11881 gatatagaat ctctaccatt tttagaagaa cctacccctt ttttagatgc taataattgt
    11941 aaattcatgt ttaacatatg gagtcacccc ctactttttt aaaattttta tattttttgg
    12001 gtattcccgt ttaatttctt taaccgccaa ttgaaagtga tttagtaata attgtgcgtc
    12061 ttttacatgt gtatctttga ctatacactc tatatgacct tcttctccta ctactttttc
    12121 aaattcaatc ttctgtaatt tgtctaaaga atttataact gcaatagcat tagatgtaac
    12181 tgcagcacat actatatcat agcctatttc agcaaaatca gcatgtccct ttaaacaaaa
    12241 accttgcaac aagaaatcat cattataata atatttaact tttatcataa cttaaccaat
    12301 aactaagcgt ttattttttc tacaactatc ttagtgtatg attgacgatg tccttgtttt
    12361 cttctgtaat ctttttttgc tttatacttg aaaactataa ctttcttagc tttaccttgt
    12421 tcaacaactt tagcttgaac agatgctcct tctactactg gagaacctaa ttttaattct
    12481 ccatctttag agcaagctaa tacttcgttt aaagtaacaa catcacctgc gttagcttct
    12541 aatttttcaa cgaataatac atcaccttca gaaactttat attgctttcc accagtcttt
    12601 actatagcgt acattaaata cacctcctcg gcctcaaact cgccagtaca aggtacttca
    12661 tttgaagatt taaaccttta ctgtgcggca ttacaattat taattataaa atatttacat
    12721 tttattgtca actattttat cgaaaataac attggtttta ttgtgcttta atgtactatt
    12781 tgcgtttaaa gaaatcttta tattatattt ttcaccaatt ttatcaatta tatccatata
    12841 atattctttt atttgttcaa aaataaaatc attaaattct accattatgt ttttgtaaac
    12901 agtatgttca cttattctca taatttcttt ttcaatacta tctagtatat aatgtacaga
    12961 ttttattgtt tgttcaccat cacaacatgg acaagacatt aaataatact tatcaatgga
    13021 ttctttttct ctacgcctag caacttctac taaaccaagt tttgtcatac ctaatacttc
    13081 agcttttctt ttatctttat taaattcttc ttctaggatg tgtaaaatct ccttttgatg
    13141 cttcttttta tgcatatcaa taaaatcgat tattattatc ccagctatgt ctctaattct
    13201 aagctgtctt acaatttctt ttgcagcttc aagattagtt ttataaacgg tttcatcaag
    13261 tttaccagtt ccaataaact tacccgtatt tacatctata accgtaagag cttctgtttt
    13321 ttcaattatt aaataacctc cactctttaa ccatactttt ttatttaata atttttctat
    13381 tttagattct attctgtata aatcaaatac atctctattt tcctcaagac acagtttatc
    13441 aacataagtc ttatctatac ctcttaaaat agacttaagt tcagagtact tatcataact
    13501 attggtaatt atagattcaa tatcatcatt cacattatct tttacatatt tagttgcaaa
    13561 atctaaagac ttatataata atttcggacc tataccaagt ttatattctt ttaaaatgtt
    13621 ttcatacttt aatttcaact cttctatatc tttctttatt tcatcatgag tgcaaccttg
    13681 agcctcagtc cttattatta atcctagatt ttccttattt actgccttag taattttttt
    13741 taatctaaat cgttcttttt catcagtaat tttatttgat attgtagttc tatcattgga
    13801 aggtatatag acaatatacc ttccagaaat actaatctca gtgtttaatt ttgcgccttt
    13861 agttcctatt tcttctttat ttatctgaac caaaatatcc tgaccagatt ttatagtact
    13921 tccctttttt aattgtaaat aagcaagttt cttaaatcca atatctacaa aacaagcttc
    13981 tattcctggt ataacttttt ttacgattcc acgatatata ttggatacag tcttcttatt
    14041 taaattgtct tcaacaaaaa gttctgttaa tctttcatct tcaagtactg ctgttttctg
    14101 ggaccctatt agggactcaa taactatctt tttcacaact tcacctctcg agcttacata
    14161 ggagtcacta attccccatc ttctaataca tataaatctc ttctcaatat atcaacatct
    14221 aatgggtcaa tatcaagttc aaacatctct aatatctttg gtataagtat attagtattt
    14281 aaattagttt ttgagcctgt agctatagta gcagtaagag taatatgttc atcttctaaa
    14341 tttaaaacat caaaggttct tatcataggt cttatatcta cttcaaccat cttacctttt
    14401 ttattctttt ttgttatgat tatttcttta ttattcataa agtcaattac ttttcttttt
    14461 acaaactctt tcgtcatagg tcttttcaaa tctatattaa acaaatattc tccatagtct
    14521 atcacagaag aaagagaagg tgtttgtcta tcaatttctt ttgctttgat aaaatctatc
    14581 ccatctggaa gttgaattga tactttattt aaaaattcct ctactgataa atcatcttct
    14641 tctatttcaa tatctacata ctcaccttga ctttctgtcc ctaaagccaa agcattgcca
    14701 taactcattt tagggtgagg attaaatcct tgagaatgtg ataagtttat ttcagctctt
    14761 ctaaacgctc tttgtaacaa tctctgaagg tcaagatgtg atatatatat catgtcgcct
    14821 tcctttttaa attttactct tattatctta ctcatattag caaagccccc ttcctatatc
    14881 atgtatattt attccacaac cattgcagtt atgtctacaa tcagaagtga ttttttcttt
    14941 tttagcattt tcgtcttctc taattaagaa ttttttacta atacctacat ctatatggtc
    15001 ccaaggaaat acctcttcac agtttctatt tctatgtgca taaaaatcta tagaaagatt
    15061 attcttttcc atagcttctt tccatatatc taaattaaaa tattctgccc atccatcaaa
    15121 tttagcacct agtctaaatg catcatatat aacttttcct attcttctat cgcctctagc
    15181 tactactgct tccatcaaac tagtctttga atcatgataa ttgtatttta tattgttatt
    15241 tcttaattta tttactaaat gtctttgctt attaataact tcttctgtag tatcttgacc
    15301 atgccattgg aaaggtgtaa atggctttgg tacaaatgta gaagtactca ctgtaacact
    15361 aaaacttctc tttagctttc ctccatttac ttttctataa atatcaataa ctttataagc
    15421 taatttagct ataccatcta aatcttcata agtctcagtt ggtaaaccaa tcataaaata
    15481 caacttaaca ctattccacc ccatctcaaa agctctttct gttgcatttt ccaaatcttc
    15541 ttctgtaaca cctttattta taacatctct aagtctttga gttcctgctt ctggagcaaa
    15601 agtaagtcca gattttctta cttgttgtat tttatcagca atttccattg aaaaattatc
    15661 taatctaagg gatggaagcg aaatcccaat attattagat gcatattcat ctaccaaata
    15721 atcagtcaac tctgataact tactataatc actcgtacta agtgatgata atgatatttc
    15781 atcatatcca gtattcttaa ctaatttatc taatatttct ttaagtcttt taacactctt
    15841 ttctcttatt ggtctgtata tcatacctgc ttgacagaat ctacatcctc tagtacatcc
    15901 tctaaataat tctagtacta ttctatcatg aacagtatct atataaggta ctattagttt
    15961 ttctggataa tctacagtct caacatcttt tattatcctt ttagtaggat ttttagtaat
    16021 cccttctcta ttaggagtta cactttgaac tgtaccatct tcattgtact tgacatcata
    16081 aaaacttggt atgtatactc cttctatgct agatatttta tataaaaaat cttctctagt
    16141 cgttttattt ttcttccatt ctttatactc atttactact tctaaattaa cctcttcacc
    16201 ttcacctaat attactacat ctacaaaatc tgctaatggt tcggaattat aagcacaagg
    16261 accaccaacc attataaatg ggtcttccaa agttctttct tcttttaata ttggtatatt
    16321 ggctaaatct aatatattta agatattagt gtatgaaagt tcatattgaa gtgtaaaagt
    16381 gacaaaatca aagtttgtta taggctctct tgactctaat gcaaataatg gtatactttt
    16441 ttctctcatt atattttcca tatcaactgc aggagaatac actctctcac aaaatacatc
    16501 ttcatcctta tttattacat catataatat atgactccct aagtgactca taccaacttc
    16561 atataaatct ggaaagcaat gtgcatatct tatcaattca ctattactag tatctttatg
    16621 aattgaattt atttcatttc caagatatct agcaggcttt tctacttttt ttaagatttt
    16681 tttcaagtct actttattca tttattaaaa ttcctccaaa caagctcttt atttttattc
    16741 ttatttataa gtaatattat acacgataaa atattttcta tcaaaaatat aactaaaata
    16801 ttgaatttaa aatacatatt attcacaaaa tagaatagct aaaaatattt aagaatattt
    16861 tctattaaat ttttattgaa atacaatttt tttacaaagt atgatatatt ataaacataa
    16921 aactattgtt ttacatgact tatcatgaca tcttttgttt cattcacctc aaaatggggt
    16981 atgttactta tataatgata aattttagga gggttaaaaa tgagtaaagt ttttgaagta
    17041 aaaaaagata tctactttac tggcgtagta gatgaaggtt taaaagtatt cgacataata
    17101 atggaaactg agtttgggac aacttacaac tcttatttaa tcaaggacga aaaaacggtt
    17161 ttatttgaca ctgtaaaggc aaattttaaa gatgagtttt taagtaattt atcagaggtt
    17221 actgatatag ctaaaattga ctatgttgta attcatcaca cagaaccaga ccatgcaggt
    17281 agtttaaaat acttattaga tataaatcct aatatagaag tttattgtac taaagctgct
    17341 aaactatact tagatggtca aataaataga ccttttaact gtcatgtaat aaaagatggt
    17401 gaaattttaa atatcggtaa aagaaatctt agatttataa ctgctccatt cttacattgg
    17461 gttgatacaa tgtttactta cattgaggag gacaagactc ttttaacttg tgatgcattt
    17521 ggttgtcact ttgcaagtgt tgatgcagag gtagtgaata gtgaagatta tctaaaatca
    17581 gctaaacact actatgattg tatagttaag ccatttgcta aacatgtttt aagtgctgta
    17641 gataaggtgg taggactaaa catagagttt gatactatat tgacttctca tggtccaatg
    17701 cttactaaag acccaatggc tgctgttaaa agatatgttg agtggtctac agaagctgtt
    17761 aatacaacta atcaaaatca agtttctata ttctacttat cagcttatag taatacttta
    17821 gagatggcta aaaagataaa agaaggactt gataaagaag gtgcaaaggc tgaattatat
    17881 gatttagaag atatgacatt aacagaaatg cacgatacat tagtagtgtc aaaagtaata
    17941 ttactaggtt ctccaactat aaacaaaact atggttaaac ctatgtggga tttattctct
    18001 gttatagacc caatggctaa ccaaggcaag atagcaggag tatttggttc atttggttgg
    18061 agtggtgaag gtataactat ggctgagact ttacttaaat ctatgtcatt caaaatgcct
    18121 gtagaatctc ttaagaaaaa attcttccct agtgaagaga cattaaaaga gtgtatggca
    18181 tttggtgcag aatttgcaaa attagttaaa tagccattaa tcaaacataa tattatttaa
    18241 aataaatgat tttatattta aaaataaatt aaaatggagt tgtctcaaaa ttaatttttc
    18301 taatctaatt ttaagttcaa ctcctttttt attttccaaa atgactaata taataataaa
    18361 tgtacccttt cgcacagggt gcattattta tagtctgttc tatttgtata ctagtatact
    18421 tgtattaatt cacgtatcta gcgtatgcta ccatccctct tccagaatta gatgccgttt
    18481 caccaatttc cactttaata attacattat tttctttaca cttttttgat aattctgctg
    18541 cagaaaggtt cttaccagta aaatctaaag aaaatggctt atcatattca aaaggatact
    18601 catcaggtac accatctgga gctaatgcaa ccataccata tggtgtattc ataaacccta
    18661 ttacagtaac tgtatgcttt gatgggtcta aatttgaatt tttgcttgta aacttaattg
    18721 tatttattga tgagttgatt aaactacacg gaactccctc atacatttca atttttggac
    18781 gtagattatt tccataaact tcatcaagta ttgccccttg ttgatctgct atcatagtgt
    18841 gattcccagg tccaactggc gcagaaggct tagctggcgt aggtttctta acagttggcg
    18901 ttggattctg tgtctttact acttcgccaa cagctggata tgctttcttg tcaaatggta
    18961 atgggtgagt aagactcttg tatatgatgt ctgctcctgc tgatttgttg gcatcaatat
    19021 ctacatgata caaacgtcca tcctcaaaac gaataagatt ccactgatgg tcatttgttg
    19081 tactatgtac ataataatat tcaattccca tagcatcaca taaagctttc aagctcctac
    19141 accttccaga acaagcacct tttttagtaa ccaatgtata agcagtattg gctctctctg
    19201 ctgcgttcca gttaacatat gttgtgttgt ttttaatgtg attatgtata gatacaatcc
    19261 actatggaga aatataaaag aaaagagtat aacaaacaat aaaaaataag atgattcaaa
    19321 ttttattttg aaacatctta tttactctac aaattaaaat tatttggtga attatttaat
    19381 tattattttt tattaaagaa attttaaatc ttaatataaa atacgctatt atacataata
    19441 atccaaatgc aaatgctact ggaattaaca tctccgattc aaaataaaag aaatattcta
    19501 agaaaatatc tggatttact atattcccat tcttgtctaa aaaatctttc ccattatatc
    19561 ctaagtgctg tataaaatta cttatgttat tataatctgc ttccctctca tacaccatat
    19621 tagcattcaa tccagaagaa cttaaattag tgtttccatc tttcatatct attacatata
    19681 gtctaagagc tttgacttta ctatacttat ctttatcaag tattctttca agttccaact
    19741 tatattcatc aatctttgtc atatcatgtc tttctgccaa ttgctttatt tctccatcaa
    19801 gttcttttcc taaattatac atattatgtt gtaaactatt attgtaatac cacataaatc
    19861 cagaaattaa aaatgcaatt atacttatgt aacctaatac ttttttaaac cccattacaa
    19921 ttgatttatg cctatccaat gataaagata gttcttttat tatattacat aattcatact
    19981 gcatttcatc accatcatca aatctttcaa ttgctttttt acatgcttct tcttcatcta
    20041 atccttctaa tttaaattca tttgcagatt ctataagatg gcatctcatt tcctgtttta
    20101 attctatagt acttttattg tcacattttt tatatagttt atctaaatat tcatctatta
    20161 gtttcataaa ttaatccacc tctcccaaaa attgattcat aaccttttta ataaatctcc
    20221 attcttgttt ctttatttct agaaaatctt taccttcatt agttaaatta taatattttc
    20281 ttcttccacc tgaactttgt tcattgctat aataagattt aattaaattt ttagattcca
    20341 ttcttttaag tgcaagatac attgttcctt cttttaattc aaactcactt ctttctctta
    20401 cttgctttgc aatttcatat ccataacaat ctttcttttc taatatagaa acaattaata
    20461 tatcgatata accttttaac acttctttat ttaactgcat tatgtaccac cgccttttca
    20521 cctactactc tataatgtat agtacttata tttactataa taatacatag tactctataa
    20581 tgcaaggttt atattctaca atagacttaa aaataataaa tagctgtata ttaatattta
    20641 atctataaat actaatatac agctatttat caaa
//
LOCUS       DAFKMX010000057        20668 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFKMX010000057 DAFKMX010000000
VERSION     DAFKMX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.057"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="LZB08_001663"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="LZB08_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBM7315168.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="LZB08_001664"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="LZB08_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBM7315169.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="LZB08_001665"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="LZB08_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBM7315170.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="LZB08_001666"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="LZB08_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBM7315171.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="LZB08_001667"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="LZB08_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBM7315172.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="LZB08_001668"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="LZB08_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBM7315173.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="LZB08_001669"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="LZB08_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBM7315174.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="LZB08_001670"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="LZB08_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBM7315175.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="LZB08_001671"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="LZB08_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBM7315176.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="LZB08_001672"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="LZB08_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBM7315177.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="LZB08_001673"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="LZB08_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBM7315178.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="LZB08_001674"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="LZB08_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBM7315179.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="LZB08_001675"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="LZB08_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBM7315180.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="LZB08_001676"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="LZB08_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBM7315181.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="LZB08_001677"
     CDS             6989..7174
                     /locus_tag="LZB08_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBM7315182.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="LZB08_001678"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="LZB08_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBM7315183.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="LZB08_001679"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="LZB08_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBM7315184.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="LZB08_001680"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="LZB08_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBM7315185.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="LZB08_001681"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="LZB08_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBM7315186.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="LZB08_001682"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="LZB08_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBM7315187.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="LZB08_001683"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="LZB08_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBM7315188.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="LZB08_001684"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="LZB08_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBM7315189.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="LZB08_001685"
     CDS             11117..11767
                     /locus_tag="LZB08_001685"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBM7315190.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="LZB08_001686"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="LZB08_001686"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBM7315191.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="LZB08_001687"
     CDS             12536..12805
                     /locus_tag="LZB08_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBM7315192.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="LZB08_001688"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="LZB08_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBM7315193.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="LZB08_001689"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="LZB08_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBM7315194.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="LZB08_001690"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="LZB08_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBM7315195.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="LZB08_001691"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="LZB08_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBM7315196.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="LZB08_001692"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="LZB08_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBM7315197.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="LZB08_001693"
     CDS             14901..15848
                     /locus_tag="LZB08_001693"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBM7315198.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="LZB08_001694"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="LZB08_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBM7315199.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="LZB08_001695"
     CDS             16330..17163
                     /locus_tag="LZB08_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBM7315200.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="LZB08_001696"
     CDS             17151..18017
                     /locus_tag="LZB08_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBM7315201.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="LZB08_001697"
     CDS             18011..18814
                     /locus_tag="LZB08_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBM7315202.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="LZB08_001698"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="LZB08_001698"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBM7315203.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="LZB08_001699"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="LZB08_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBM7315204.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="LZB08_001700"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="LZB08_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBM7315205.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8254 a         2443 c         4318 g         5653 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt gtcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaagtgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatctta
//
LOCUS       DAFKMX010000058        20588 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFKMX010000058 DAFKMX010000000
VERSION     DAFKMX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.058"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            212..1429
                     /locus_tag="LZB08_001701"
     CDS             212..1429
                     /locus_tag="LZB08_001701"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBM7315206.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1524..1904
                     /locus_tag="LZB08_001702"
     CDS             1524..1904
                     /locus_tag="LZB08_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBM7315207.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2188..2670
                     /locus_tag="LZB08_001703"
     CDS             2188..2670
                     /locus_tag="LZB08_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7315208.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2691..3506
                     /locus_tag="LZB08_001704"
     CDS             2691..3506
                     /locus_tag="LZB08_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7315209.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3556..5106
                     /locus_tag="LZB08_001705"
     CDS             3556..5106
                     /locus_tag="LZB08_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7315210.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLSVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5203..6555
                     /locus_tag="LZB08_001706"
     CDS             5203..6555
                     /locus_tag="LZB08_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBM7315211.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6686..7444
                     /locus_tag="LZB08_001707"
     CDS             6686..7444
                     /locus_tag="LZB08_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315212.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7491..8210
                     /locus_tag="LZB08_001708"
     CDS             7491..8210
                     /locus_tag="LZB08_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBM7315213.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHISKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8334..9734)
                     /locus_tag="LZB08_001709"
     CDS             complement(8334..9734)
                     /locus_tag="LZB08_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBM7315214.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9738..10646)
                     /locus_tag="LZB08_001710"
     CDS             complement(9738..10646)
                     /locus_tag="LZB08_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7315215.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10907..11554
                     /locus_tag="LZB08_001711"
     CDS             10907..11554
                     /locus_tag="LZB08_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBM7315216.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11879..14518
                     /locus_tag="LZB08_001712"
     CDS             11879..14518
                     /locus_tag="LZB08_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBM7315217.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQVILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14576..15679)
                     /locus_tag="LZB08_001713"
     CDS             complement(14576..15679)
                     /locus_tag="LZB08_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBM7315218.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16039..16860
                     /locus_tag="LZB08_001714"
     CDS             16039..16860
                     /locus_tag="LZB08_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBM7315219.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16939..17150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17237..18553
                     /gene="argH"
                     /locus_tag="LZB08_001715"
     CDS             17237..18553
                     /gene="argH"
                     /locus_tag="LZB08_001715"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBM7315220.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18802..19140
                     /locus_tag="LZB08_001716"
     CDS             18802..19140
                     /locus_tag="LZB08_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBM7315221.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19267..20556)
                     /locus_tag="LZB08_001717"
     CDS             complement(19267..20556)
                     /locus_tag="LZB08_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBM7315222.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
BASE COUNT         7833 a         2420 c         3559 g         6776 t
ORIGIN      
        1 agaatgtaaa aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata
       61 tgtttattgt atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa
      121 aaatcgttta catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa
      181 acaaaaattg gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtaaa
      241 agaggctaga gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa
      301 acttagcgaa aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg
      361 ttcttttaaa ttaagaggag cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc
      421 aaatggagtt atagcttcaa gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa
      481 aatgactggt ataaaatcta caatagtaat gccagcaact gcgcctttag caaaagttag
      541 tgcaactaaa ggttatggag cagaagtagt tttaaatggt gcggtttatg atgatgcata
      601 tgcaaaagct gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga
      661 taagtatgtt atagcaggac aaggaactat atctcttgaa attttcgaac aattagacaa
      721 taaagttgat actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt
      781 tgcagcaaaa gcattaaatc ctaatgtaaa aatagtcggt gttcaaactg caaatatacc
      841 ttcaatgaaa gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat
      901 agctgatggt atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt
      961 ggttgatgaa atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat
     1021 ggaaagtcaa aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag
     1081 tggtaagtat gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat
     1141 agacataaat acgttatata gaataatagg tgttgcttta gctaaagaag gaagaagata
     1201 ttcatttagt acaattatgg aagataagcc aggaaacttt gctgagttaa ctagaataat
     1261 aagtgagaat ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc
     1321 tctaggaaaa caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag
     1381 aataaagaaa gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta
     1441 tataatatac aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat
     1501 taacgaatta ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag
     1561 cagctttagg accatattct caagctataa aagctggaaa cttgttattt gtatctggac
     1621 aagttcctct tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg
     1681 ctcaatcttt aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg
     1741 tagttaagac tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt
     1801 atgctgaata ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac
     1861 caaaagacgt taaagttgaa atagaagtaa tagctgtatt ataaaaatac aatattttta
     1921 atttgaaaag gctgaaaaac tatattctag tgattcagcc tttttataaa aaaataaatc
     1981 acaataaaga acatgataaa tattgtcgaa ttaaaaaata aaattaaaat aaatattgca
     2041 atatttttga gaaggtttta taatgagtat ataatttgaa acgtgtaact attaaattag
     2101 gcatgagttc ttggatattt ttatcctgag gattcatgcc ttttttgtat tgaaattcaa
     2161 aaatctattt aataaggagg aagtactgtg ttaaattttt tcaagaaaaa taaaagctat
     2221 aagttacatg cagtagtgag tggtaacagc attaatattg agaaagtaaa cgattctgtg
     2281 ttttcaaaaa agttaatggg agatggagtt gcaattattc ctaatagtga tgttgttgta
     2341 gccccttgta atggtaaagt aactgtgctt acagaatcaa agcatgcctt tggtatggtc
     2401 tcagatgagg gtgttgaaat attagtacac ataggaattg atacagttag tttacagggt
     2461 gaggggttta aaaatgaagt gtctcaagga gatactgtta aaaaaggtag tccaataata
     2521 agttttgaaa gagaaaaaat aaattctcag ggaattgatt gtacaacaat aataattgta
     2581 ttaaatcata gcgaattttc agaaataaat tgcatggtag aaaatgaagt tgttgcaggg
     2641 caagatactg ttattgaaat tatgaaataa taggaaggga ttaattcaat atgagagtag
     2701 taaaaatctt taataataat gcattatcta cagtaactaa tgataggaaa gaagctattt
     2761 tactaggtct tggtataggt tttaataaac gtccaggaga taaagttaat gaagataaaa
     2821 tagaaaagat ttattatgtt caagaccaca tgcaaacaaa atttttagaa ttattaaaaa
     2881 atgttagtcc agaagtgatg gatgcttcac aacaaattat aagtcttata ccaggaaatg
     2941 agggaaaatt taataataaa gggatattat ctttagtgga acatattagt tttgcaatag
     3001 atagaatgaa aaataatgtt tttttaccta atttaatgct tttggatata aagatgatgt
     3061 attcaaaaga gtttgaatta ggagtaaaag ctttagaaat tatatataga gtatgtcata
     3121 tccatttgcc tgaagatgaa gcaggttaca tagctttaca ttttgtcaat ttacaatcaa
     3181 atgataattt ggcatatgat actttaaaat ttgtaaaagg tagtattgat ttgattaaag
     3241 aatgttatgg attagaatta gacgaatcga gtttaagtac attacgtttt agaactcatc
     3301 ttaaatttct tgctcaaaga atttttaaga atgaaatttg tcaggatgat aaaatgattg
     3361 aaatgtatga ttatttaatt aacaatcatc ctaaaaataa agaatattta gaaaagttaa
     3421 atgtatatat tgaaaaggag tttaggtata aattagataa acctgaaaaa atttatctac
     3481 tactacacct aacaaaaatc ttataagaat gagatgaatt agtaatagaa atatataatt
     3541 ttagggggga taagattgaa agaaaaacta cttggaggac tggaaacatt tgcaaaggca
     3601 atggttcagc cattaatgta tttatcgagt gcaggaatat taatggtggt aggtgtacta
     3661 cttacaaatg atatgttatt aggagtactt ccatttttta aatgggagcc aattcaaata
     3721 tttggaagcc taatttataa ctgtataatg gctattatta ataatcttgc tgttatattt
     3781 gctataggta ttcctgctgc actagcaaaa aaagagaagc acaaagcagc gttaataggg
     3841 tttatgagtt atataatata ccttacagct tctaacacta tgttaacatc actaggtcaa
     3901 ctagcaaaag aaagcaagat gcttggactg atagggactg gacaagctac tattttaggt
     3961 attcaagttg tagatacaag tgtatttggt ggaattattt tagggtgtat agctggatat
     4021 gtatttaatc gtacaagcaa caaaacattt aaaggagctt tacagatata ctcgggagca
     4081 aattttagtt ttatatgcat gttctttgta tccattttaa ttggagttgt gagtaacttt
     4141 atatggccat tagctcaaca aggaatagct tctcttgcaa atgtcatcaa agatacaggt
     4201 acatttggtt tattcttata tggtttttta gagagaatat tagtaccaac tggacttcat
     4261 catttaatct atacaccatt tcagttttct gatttaggag gagtgcttaa agttggtgca
     4321 gatacagttt caggagccta tgctatagtt atgacagaaa tgaatttatc agttgagcgt
     4381 ttctcagata gtatatacta catggcaact ggatttacaa agatgtttgg ttatataggt
     4441 ataggagctg cctttattta tactgcatat aagcaaaata agaagaaagt taaggcaacg
     4501 attattccac taataattac ggcagtatta gcctctgtaa cagaaccatt agactttatg
     4561 tttgtattct gtgcaccact tttattcttt gtacatgcag ttatagctgg tctttttata
     4621 gctttattaa aaatatttga tgttactgca ttatgtggag ggaacttatt agtatcaatt
     4681 ataatgaata ctacagtagg cgtagaaaaa acacactggc cagtaatgat gatattagga
     4741 cttgcacaaa ttattgtata ttttgtagta tttagttttt taattaagaa atttaattat
     4801 aaaactccag gtcgtgaaga tgagagtaca gctacaggcg aagaacaatc aaaacaactt
     4861 aatttagatg caggtataga aaatataatt gatgggttag gcggaaaaga aaatattaat
     4921 acagtagaaa attgcatcac tagattacgt gttaatgtaa aagatgaaag taagattaat
     4981 gaagatataa ttaatttaac gccaaatagc gggattgtaa ggaaaggaaa agatatacaa
     5041 ataatttttg gacttcatgt gcatgaggta agacgtgcag tagaagattt tttagaagct
     5101 tattaaagat atatatttat atagcaaggg taaataacag ctttaagtgc tgtttactat
     5161 ttattaaaga tacatataat ttatagagga ggaataatta ttatgattat tactattgtt
     5221 ggtggaggaa gcacatttac tccaggtatt gttaagtcaa ttgcattaag agaaaaagag
     5281 ttagagcttg aggaaattcg cttatttgac attgataaag aacgccaaga taaagtagct
     5341 gtagttgtca actggatact aaaagaagat gttaaaacag atgttaaact aactgtaact
     5401 aattgtgaaa aagaagcgta tacagatgct acatttatat ttgcacaaat gcgtgttggt
     5461 aaatatgaaa tgcgtgagca ggatgaaaaa attccattga aatatggttg tgtaggtcaa
     5521 gaaacttgtg gttgtggagg gatggcatac ggactgcgta caatacaacc aatgataaag
     5581 gttattgatg atgtagagaa atatgctaaa aaagattatt ggatattaaa ttactctaat
     5641 cctgctgcaa tcgtttctga agcttgtcgt aagttaagac caaatgcaag gattataaat
     5701 atctgtgata tgccaatagc tattatagat atgatagcgg ggtcattaaa tatcaatgac
     5761 gttcataata tacgctatga ttattttggt ctaaatcatt ttggctggtt tacttcaata
     5821 gattataaac atagagactt aatggaagag attaaggaat acataaaaga aaataaaata
     5881 ttattaccaa aatcttatat agaaaactta aataaaagtc gtagtacagg aaatcgccat
     5941 gctgtaagta gctggataca tgtttgggaa tctgtttata caatgttaga aagcttccca
     6001 aaatatctgc caaacactta tttaacatac tatcttttaa gtaaagaaac agtggaacat
     6061 tcaaacataa atcgtactcg tgcaaatgag gttatggaaa gccgtgaaaa agacttattt
     6121 gaaggtgtga gacattactt agaaactgga gaaatttctg aaaaagcatt ttatgcaggt
     6181 tcacatggag attggattag tgatttggca gtttcaatta aaaatgatac acgtagtcgt
     6241 tttttagtta taactgaaaa tcgtggagct ataccaaaca tgccatatga tgcaatggta
     6301 gaaattccag catatattgg taaagatggt ccagaagtta ttgctcgagg tccaatacct
     6361 ctattccaac aaggattaat gatgcaacaa cttaacagtg aaaaattgat agtagaagct
     6421 tatatagaaa atagttatga aaaagcttta caagcattca cattaaataa aactattcca
     6481 agtatgaatg ttgctaaaga aattttggat gaaatgatag aagttaataa ggaatactgg
     6541 ccagaattaa gatagtagga taaaagaaag ggtttttatt taataattag atatgttttc
     6601 agagtctttt atcaaaatgt attcacaaac atatggcaat atattaaaat aaagagtgat
     6661 atttttagaa gaatggggtg atgcaatgaa cattataaat aaaatttttt gggaagaaga
     6721 taggattggc atgattacaa ataatttaga ggcagataca catagccact gtatgttaca
     6781 actgttctta gggatagaag acagcattga aataacagtc aatgaaaaat tagttaaatg
     6841 taattgtatc atagtagata aaaatatctc tcattcattt tcagcaagaa aaaaagttta
     6901 ttactctgct attattgaac ctacatctat atatgcagaa cagcttacct caaaaatgaa
     6961 tgattttggc tactggatat gtgacaatga tggattagag aaattacgtc aacaaggaac
     7021 ttttttgata gataactcta gtaaagaaca atatttaaga tttatggaaa tgcttaataa
     7081 ctatttgaat atacctataa ctctcaaaca ttatgatgac agaattactg aattgctcaa
     7141 tttattacgt acttgtaatt gtgataatca tactatttca agttttgcag ataaagtgtc
     7201 cctatcagca agtagattat cacatctgtt taaagaacaa atagggatac ctttaaaaag
     7261 ctatatctta tttcatcaaa tggagtgtgc ttttagagaa ttactttctg ggaaaaatgt
     7321 taccgaagct tctatgactg ctggattcga tactccatca cactttgcag ggacagttaa
     7381 gcgaatgatg ggaatgcctg tttctttatc tttgaaagat agcgaatttt tgaaagtata
     7441 ttaaatattc ttgttatata atgaagttgt actaagaaag gggctttgtt atgaataaag
     7501 aatatttaga ggaaacaaaa atgttaaatt ataatgaacc acaacttaag cttttggtat
     7561 catcaaagaa ttggttggaa ttagatgatt ttcataaaat aaaatctatt tatgagtttg
     7621 tacagaatga tattttattt ggatataatg cttctgatat gttaagcgca acacaggtat
     7681 taaatgatgg tatgggacaa tgcaatacaa aagctactct tttaatggca ttattacgtg
     7741 cagtgaatat cccttgtagg cttcatgctt ttgatgtaac aaaagatttt caacgaggag
     7801 ctacatccaa attgatttca ctattagcac caaaatatat tcttcatact tgggtagagg
     7861 tattttatca agataggtgg attgctctgg agggtgtcat aactgataaa aaatatttag
     7921 aagcaattca gaagaaattt tttaatcatg gtggaacgtt taaaaagtat gcaatagcta
     7981 caaatgattt gaaaaataca tctattgatt gggatggtaa agatactttt atccaaaaag
     8041 aagctatcgt gtatgattat ggtatttttc catcaccaga cgtatttttt tctactcatt
     8101 cacaacatat ttcaaaatta aaaaatttca tatatgtaca tcttatcaga aaaataatga
     8161 caaaaaatgt atgtaaggct agaaacaatt atattgataa aaacgaatga gtgtttaaga
     8221 ggtaactata taggaaatat atagaataat taaaactgta ctggttaaaa tgaataaaaa
     8281 ggagttatca cagagcaaac ttaaattgtt cataggataa ctccttttct gtattaactt
     8341 ctaataattt gatgtctttt tataaaacat atagatacaa ttaaaccagt aaatgcaaga
     8401 gtagctgcta ttgaaaatgc aacatctatt ccatgaatgt tagacagcat gtttgaactc
     8461 atatgagctt tcttagtagc acttgtcatt atagtaataa gtatagcaga acctatagct
     8521 ccagatatct gacggagtgt gttattaatt gctgtggcat gagatataag ttctctatct
     8581 aatgttttaa ttccccatgt agttaatggc attaaaacca ttgaaatacc aatcatacga
     8641 atacaataca tcaatgacac atgaattaaa gatgtatctc tgcctaaaaa agcgaaggat
     8701 aaagttccta aaaacaaaca tcctgttcct aatattgata gagcatgagg tccatattta
     8761 tctaaatgac gtcctgcaat tggattgagt acaaccatca atatagcacc aggtaacatt
     8821 aatgaacctg aagataatgc agaaaatcct cttacagatt gaacgtacag aggaatcatt
     8881 aatgtagcag acatcattga ggcatataca atcataatta atattgtaga aataagaaat
     8941 ggtttatttt taaaaaccct caattctaaa aaaggttctt tagattttaa ttgacgcaat
     9001 gtaaataaaa ttaatgacat aattcctatt aatattggta aatatgtggc aatattagtc
     9061 cacccataat taccttgatt agtaaatcca attaataaac caccaaaacc tagagatgat
     9121 agtactattg aaggtatatc taattttagt ttttgagttt ccccaacatt tcttaataga
     9181 ataaatgata gtataacatc taatatagca ataggggaaa gaaacagaaa taaactatgc
     9241 caaccaaatg aatctactaa ccatcctgat aaagttggac ctattgctgg agcaaatcct
     9301 actgtaatac caactaaact catagctgca ccacgttttt cctcagggca taaatagagt
     9361 ataataactt gtaaaagtgg cattagagaa ccagaaccca ttgcctgcaa cacacgagca
     9421 atcaacatag ttgaaaaatt attagaaaaa agtgcaataa tacatcctat agaaaagata
     9481 gacattgatg taataaataa ctttctagta ctaaatcgat ttattaaata agctgttgtg
     9541 ggtacaataa tacctgcaat aagtagataa attgttgtta accactgacc aatatcagca
     9601 gtaattttaa aatctgacat tatattaggt aatgctgttg acaatagtgt ttgactgaga
     9661 gaacttataa aagcaccagt cattacaatt gctattatta aatttccttt tttattgtta
     9721 ctttgggttt ccataattta taacgtctcc ttttttaatt gatttttaat gctgttcaat
     9781 aaaagtactt ttggtcttaa agtgtcttct ttttctaaat atgcaatcat tgcatccata
     9841 attaaagaat catattgttg attttttccc tcgacgtata atttttttat tgcttctagt
     9901 aatttaggat tttcaaggag agaagtatca gttttgatta gttcttctat atcttgtatc
     9961 gttttaaaaa atagcgattt attatgaagg gaaaagtaaa cttcatcttc ttgtttatgt
    10021 aaaaacattg atttttcaat taaaggattt agattatcca tattttctac aactaaatct
    10081 agaatttgta tagtaaattt ggcaataaag tatacattta tttctctttt ttgtacatga
    10141 ataatgtata taaactgatg aattagagca tccaataaga aaactaaatc aaatataatt
    10201 ttttctattt tatcaccata gttatcaaat aagcttagtt tgtattcatt caataagtta
    10261 gctcgtgatt ttgtttgcaa tttacttgta gccttacgtt gctcttcaga aaaattttct
    10321 cttatatagg tatttatggt agttctagaa aaaatatttt tccatacagc aattatcttt
    10381 ctgtttaact tttctgatga tggaatgtca ttattttcgt ttatggaatg tataatttct
    10441 aaaaactttt catcataata attgattatt tcgcagataa aactttcctt agtttcaaaa
    10501 tgcttgtaaa aagtagcttt agacatttta cactctttta caacatccga tattgaagta
    10561 ttttgaaaac ccttttggtc aaaaattttt tgtgcaatag ttataatgag cctttgtttt
    10621 tctgtcactt tattatctcc tttcacgcaa aaacgtttag tactatatag tacctacaag
    10681 ttttaataaa tatattatac atcatttgga aaaaaaatca atatatagac tgtaatatga
    10741 aatttttgaa aaatttgttg ttatttttag tcgtataaaa aagaaactta agagtttctt
    10801 aagaattgtt taaagaagtt gcaataaatt agtggtatga ttatagtatg aaagagaatg
    10861 tcttgaaatt tttctgtatc aaattccaat tatggaggtg tagtatatga aaaagaaaat
    10921 ttgtgtagca ataatttttg ctctatttat agtttttgta gaaatgttta ttgatagctc
    10981 tagcgaagga agtaaaatta tgacagctga attaagtaaa tacaggaaag gcgtaaatgt
    11041 attatcaata aacaatatta tagtagcgaa taaaaaatat agtttgccca aagattattc
    11101 tcctcaggaa agcagtgaag cgagagatgc tttttataaa atgaataaag atgctcaaaa
    11161 gtcaggatta aatttaaaag cttttagtac atatagaagt tatgaatatc aagatagatt
    11221 atttaagtca tatgtaaaag aacatggaga aaaagaagca aatagatttt cagctaaacc
    11281 tggagagagt gagcatcaaa ctggacttgc ttttgatatt ggaggagatg accagtcttg
    11341 ttgggcaaat aaaaagttca acaatacaaa agaagctaag tggttgtatg aaaatgcata
    11401 taaatatggt tttatactta gatatccaga aggtaaagag aatataacag gatatatgta
    11461 tgaatcttgg cactatagat atgttggaac tgaacatagc aaaaactttg caatgaataa
    11521 tttgacttta gaagaatatt tacatataaa ttaagagatg gtttgttaaa attaggttaa
    11581 aataaaaaat aacctagact attagctgtg taaagtgata taatgtaaat acaatttaag
    11641 ataactactc gaaggggagt agcttacacg aaagtggtaa ttaatcgtca agacagtaga
    11701 aatactcggt taattagcaa gggattcctt gatagcaaga ccttcaaaat aagcattagg
    11761 tatgtttgtt ttggggtctt tttttattca aggaattttt atttaaaaaa atttaaattt
    11821 caagtataca taagagcccc caaacataaa ataaatttat tgaagaggag ggcaacaaat
    11881 gtcttacaaa agtacttatc aagaagtaat taaaaattta gactcaagtt tatctggttt
    11941 gagtaaagaa agagcagaag agttgttaga aaaaaatgga gctaatgaat taaaagaagc
    12001 agataaggtt cctacttata agcttttcct agaaagtttt aaagacccat tagtaataat
    12061 tctattgata gctgctttag tacaaatatt tttaggtgaa acagtggagt ctataataat
    12121 ttttgcagtt attattataa actctgttct gggtgttgta caaacaaaaa aagcagaaag
    12181 ttcattagaa agtttgaaaa atttatctgc accaaacgca aaggtaataa gagacaataa
    12241 aaaaatgaca attccagcaa aaaatctagt agttggagat attgtattct tagaagctgg
    12301 cgattacata ccagcagatg gaaggcttat agaagcacaa tctctcaagg ttgtagaagg
    12361 gatgttaact ggagaatcag aaccagtttt aaagcataca gaaaaaataa gtgaagatgt
    12421 aagtttaggt gaccagaaaa atatggtatt tagtggtgct gtagttgttt atggtagagg
    12481 ttcttttgta gtgactggaa caggtatgga tactgaaatg ggtaaaatag ctgggctttt
    12541 agaaagtgct gaaaataaac ttacaccatt gcaacaaaaa atagaggatt ttagtaaaaa
    12601 acttggtgta ggtattgtaa tacttgctct tttaatattt ataattcaag tagcaagaaa
    12661 ttactttatg gctggaaatg gagaaatgac acaagttatt ttagattcat tcatgttctc
    12721 tgtagcagta gcagtagcag ctataccaga agctctatca tcaatagtca ctatagtatt
    12781 ggctgttgga acaaatagta tggcaaaaaa acaagccata ataagaaaat taccagcagt
    12841 agaaacttta ggttcaacaa gtgtaatatg tactgataaa acaggtactc ttacacaaaa
    12901 taaaatgaca gtagttgatt attatatgta tggaactaat aatgatgagt tagataaaga
    12961 aaaagtaaac tattcttatc atgctaaatt attgactatg gtttctacat tgtgtaatga
    13021 ctctcatata actagtgatg gtaaagaagt tggtgaccca acagaagtag ctttaataaa
    13081 ttattcaagt aaaaatgatt tagattatga taaattaaga agtaagtata tcagaataaa
    13141 tgaaatacca tttgactcag acagaaagct tatgtctact gtaaatagta tatatggaga
    13201 ttatataatg tttacaaaag gtgctccaga tattgtgttt agtagatgta aatatgcttt
    13261 gaaaaatggt tcaaaagtag atataacaga tgagataata gaagaatata aacataaaaa
    13321 tgaagagttt tcaaatagag cacttagagt attagctttt gcaataaaag atgtcccaga
    13381 agaagatttt ataccttcaa ttgatgacga acatgacatg acattggttg gaataatggc
    13441 aatgatagac ccaccaagag aagaagtaat ggatgcagta aaagaggcaa agagtgctgg
    13501 tataaaaact gttatgataa ctggtgacca taaaacaaca gcagcagcaa ttgcaaaaga
    13561 gattggtata atggaagaag gagacttggc tcttactgga aaagagcttg atgctttgag
    13621 tgaagaacaa ctatatgaaa aactagaaaa tataagtgta tatgccagag tctcgccaga
    13681 aaataaaatt cgtatagtaa aagcatggca acataagaat aatataacag caatgactgg
    13741 tgatggtgtt aatgatgcac ctgcattaaa acaagcagat ataggaatcg gtatgggaag
    13801 tggtactgat gtagcaaaag atgcttctgc tatggtattg gttgatgata actttgcaag
    13861 tatagtaaat gctgttgaag ttggtagaac agtatacagt aatataataa aatcaattac
    13921 ttatctattt gcaggaaact taggagctat agtatccata gtatttgcag tgtttgcagg
    13981 ttggtcaaat ccatttacag ctttacaact tttgtttata aacttagtaa atgactcttt
    14041 accagcaatt gcattagggt ttgaaaagtc agaaaaaaat ataatgaata aacctcctag
    14101 acacccggat gaaagtatac tagcaggagg aacattgaag gcagttgcta taagaggttg
    14161 tataataggt gtagttacaa taattgcaca atatatagga ttacaagtat ctccagaact
    14221 tggagtagca atggcatttt caacattgat attagctaga ataatgcaaa cattacctgc
    14281 acgttctaat aatcaaacag taataaaatt aggttttttc agcaataaat acgttatagg
    14341 agcagtttta gtctgtcttg ggctttactc tttaacacta ataccagtgc taagaggaat
    14401 attctctata ccaacaacgt ttggattgta tcagttgggg atttgcctag ggttagcatt
    14461 aatttcatca atattaatgg aacttaacaa attaataaat agaagtgaag taaaatagtc
    14521 aaaaaattaa agggcatatt gattttaaca atatgccttt tataaatagt ctaatttatt
    14581 ctatattatc gattatttgg aatgctttta taacttcagg taattctttt tcttcaggca
    14641 ttctaaatct tatgaaattt ttatcaagtc cagtaaaatc acttccagat ataactctta
    14701 tttttaattt agcaaactct tgttgtaaat ctatgtcctt gtttttatgt tcaactgtca
    14761 taattgatac agtatcacta gtttcagcta tattaaggtt tttccatgga attaataatt
    14821 gattttttat atcttttgtt ttttctttta attcttctat aaattgaaca tcatctaata
    14881 aatttgctgc aatactacga gatatttcag acacttcgta aggagtagaa atctttttaa
    14941 tagggctaac aagttgttct ggaagaactg catatcctgc tcttagtcct gctaaaccaa
    15001 atccttttga aaatgttttt aaagcaatta cattatcata attatttaga agcttgacag
    15061 ctgaattttc ttttggcatg tattcaccat aagcttcatc taccataact gctatatcat
    15121 atttagctgc ttcctttact atgttttcaa ttgatgataa aggtataatt tgtcctgtag
    15181 gattgtttgg attgtctatg taaattactt tatattctgg atttattttt tcaataaatt
    15241 ctttttcatt aaacttgaaa ttatcctctt tctttaaaag aacataatca taagttgcac
    15301 catgcatttt tatatctgtc tcatattctg agaactgagg tgaatatcct aaaactttat
    15361 ctcccttttc aataaatagt ctatttaaaa ggtaaataac atgtatagaa ccatctgcca
    15421 aacaaattct gtcagtatct aatgctataa aatctttcca ataatcgact atattatctt
    15481 ttaaagcaat tgaatgagga tatcctctta tcatatcaaa ttttaaagca ttaaaagctt
    15541 ctacagcttt gtttgagtaa gacacggtat ttataccttc accacagtct atctcgaata
    15601 catcttctga atgtccagat tctattgcat aacttttttt caactgcatt tcaatagctt
    15661 tgttaatttt tagtttcata ctttatcact ccttatttag tcaataataa atgttataaa
    15721 agttgctaga tttaaaatat tatgaaaata tataatagta aaaatgaata taaatattat
    15781 aatagctcac tttatttaat atgattatat atcatatatt gtaaaatttc tagcattata
    15841 agactataaa ttgaaaatat tcagaaaaat actacaagaa acgatttatt ttgctcttaa
    15901 aataagttaa atgaatgtta ttaattttgt aaattgaaat agtttaagta taaagttatg
    15961 atataatcta ataagggtaa ttctttagat tatattaagg aaatataggt atacaataaa
    16021 aagttatgga gttggattat gggatataaa ttaattgtaa cagatatgga tggaacttta
    16081 ttaggcaata atcataaagt tacagatgaa aataaaactg cactacaaaa agttataaaa
    16141 agcggtatta atgtaacttt agcaacagga agagccttcg attcagctaa atgcaatgta
    16201 gattttctta aagaggacat gccaataata gcttgtaatg gttcattaat aagagagcaa
    16261 aatggaaata taatatattc aaataagatt gatacaagaa catgtttaaa cattttagat
    16321 gtattagata aatacgatat ttactaccaa tgtaatagta tagattctat gctttcgaaa
    16381 aaaatagaag gacgtgaaga tagattaagt gtatttttag gctctgaaac agaagtaatt
    16441 gtaaaagatg atttgagaga agaaatcttt aaaaaagata tactaaaatt tgtaatcata
    16501 gaagaaaaaa atccatctat tttggatgaa ataagaaaag aattgagaaa agttcaagga
    16561 ataaagataa cttcttcatg gccaaacaat attgaagtca tgaatgaagg tgttgataaa
    16621 ggtaatgcag ttaaaatttt agctgaaaaa atgaatatag acagagaaga tataattgca
    16681 tttggagaca attacaatga catagaaatg ataaagtttg ctggtcttgg ggtagctatg
    16741 ggaaatgcag aagaattgat taaacaagaa gctgattatg ttacagatac aaatcaagac
    16801 agtggggttg caaaggcaat ttataaattc ctagagttaa aagaatcact tagctcttaa
    16861 aacagtatat aataggaaaa aataggttga ctattttgta gatagtaatt ataatacaaa
    16921 tagataaaac ataaaaattt agattcagtg aaaaagagag tagatataga gaaaatctca
    16981 gcgaattggg ggatggtgtg agcctaatga taacacttta tattgaaaag aactttggag
    17041 aatactactt gaaattcagt atggtagtag gtaatctcgc cttaagagaa tagagtgggt
    17101 aaacattgtt taccaactag agtggtaaca cggaatattt cgtctcttgg tctttttgac
    17161 caagagattt tttagtttca ttgagttaca agacttataa ttaaaataca aaaaattaaa
    17221 atatgaggtg aaagttatga aattatgggg cggacgcttt agaaaagcgg aaaatcaact
    17281 tatggaagaa tttaataaat cttttggcta tgattgtgta ctgtacaaaa aagatatcga
    17341 aggaagtgta gcacatgttc atatgcaagt aaaatgtgga cttttaacag aagaagaagg
    17401 aaagtctata acagaaggtt taaaagggat acttgaagat gtagaaaatg gaaagttagt
    17461 attggatgga gagtatgaag atatacatag ttttactgag ataaatttaa ttcaaagaat
    17521 tggagatgtt ggcaagaagc ttcatacagc acgtagtaga aatgaccaag ttgcagttga
    17581 tatgagattg tatgctaaag aaaaagcaaa tgatttggtg gatttaatat ctgaatttaa
    17641 agctactata aaagatgttg ctgacaaaaa cccagtgatg atgccaggct atactcatct
    17701 tcaaagggca caagtagtga catttaagca tcacttaatg gcatattata gtatgtttga
    17761 tagagatgag aaaagaataa aaaatgctat agaaatatta gatgaatcac ctttaggctg
    17821 tggagcttta gcaggaacta cgcatgatat agatagaagt ataacttgtg aacaattagg
    17881 atttaaaaaa gtagtagata attttatgga tggagtaagt gatagagatt atttactaga
    17941 gctaatgagt gatttttcaa ttataatgat gcacttaagt agattaagtg aagagttgat
    18001 actttggagt tctcaagaat ttggatttgt tgagatagat gatttatata caactggaag
    18061 tagtataatg cctcagaaga aaaatccaga tggtgcagag cttataagag gtaagactgg
    18121 tagagtatat ggaaacttat ttggtctttt tacagtaatg aaaggaatac cattagcata
    18181 taataaagac atgcaagaag ataaagaagg ttttttcgat agtgtacata ctcttgaaat
    18241 gtgtatacaa ataatggata gaatgatagc cacactaaag gtaaatgagg ataaaatgaa
    18301 acaagcagta aaaaatggtt ttttaaatgc aacagaagtt gcggattatt tggttaaaaa
    18361 taatgttgct tttagagatg cacatggtat agtaggaagt atcgttatat attgtgaaga
    18421 taataaaaaa gcaatagaag atttaacatt agaagaatta cataagtttt cagatgcttt
    18481 taaggaagat atttatgatt ttatcgacta tgagagtata ttaaataagg gaataaaaaa
    18541 gaatttaaaa taatgtattt atttttaaaa gctgtaaatt tgaatgttat catttaaatt
    18601 tacagctttt tattgtgtaa gattttatta tataaaagta agaaacttaa aaggagtagt
    18661 atttaaaata aatacaaata aaaaattatt attaaaatga taattaatat caataagtat
    18721 tgacaaaatt atttctaaat actattatat ataatataaa taataatgaa aatcaatatc
    18781 aaataaatgg gaggaaatct aatgaactta ttagtgattg gtggtcatga aagaatggaa
    18841 aaagattata tggttatggc caagaaaaaa ggttataaga caaaggtata tactaccatg
    18901 tcatctaaat taaataattc tataggaaga ccagatgcag ttgtcatact tacatcaact
    18961 gtttcacata agatgtctag aacggttgag tctcaagcta aaaaacaaga tatattaatc
    19021 gtaaggcata aaaatagtag taaggtagca tttaatgaat gtctagatat ggttgatgaa
    19081 tgtctgggaa attgttcaaa atgtaagctt aaagacaaac aaaaacaaaa aaatatatag
    19141 gtataaaaaa tacataaaca taaaattaaa agtactgaga taatagttat aattattatc
    19201 tcagtacttt tagatttata aggataagta tagtataaat tttttattat aatttccaac
    19261 tacttgttac tttttcatat tttttttact tctagctttt tttctttttt tcttctttat
    19321 atttgaagct attataaata aaactaaaag cactagaata cctgcaataa tcttaacaaa
    19381 agtcattaaa acattatttt cagtcaaaaa tggaagactg ctatcaagat tattttttgc
    19441 aattaagtct atactgtttt ctagtttacc attattatat acatttaaag taccaacctt
    19501 agctcctttt tttataggca aatcaatttt atttaactct gttttggtat cataattgcc
    19561 ttgagattca tctttttcta agaatatcct ataactattt tttggttcat aaatcaattc
    19621 atgttgtttt gtaaacaata cttttttagt atctgtgtat ttttctttat caacaatagt
    19681 agcacactta aagttatcaa atccataatc aatcagaata cgtgagtcta aatataaatc
    19741 agcattagtt gaattaaata cagcaacgat aactcttctg ccatctttta cagcactcga
    19801 taaaagacat ttaccagcag catcagtata tccagttttt ataccatcaa ctatatcata
    19861 ttttatattt ataggttgac ctttgtatgt aatcttatca tgtgatgtca aaaacttatt
    19921 agtgtttaca aagtatctct cataaggata tgcttttgta gcttcaaatt taagtgactt
    19981 agtttttaca atttgtctaa aagtatcatt attcatagct tctctagcta tgagagccat
    20041 atcatgagct gttgtgtagt gatttgggtc tggtaatcca tgaggattat taaaatgagt
    20101 attagtagct ccaatttttt ttgcctcaga gttcataagg tcaataaatt tttgtacatt
    20161 gccaccacca acatatctag ctaatacaaa agcagcatca ttagcagaat gaaccaaaag
    20221 agcatctagt aactgcttta cagtaaaaga ttccccattt tttagataca tactagagcc
    20281 atctatttga ggtaaatctt taatttctat tacttgattt aaatctttaa cctcctttag
    20341 aactaaacaa gctgtccata ctttagttgt tgaagctgga taaagcttag aatttccatc
    20401 cttattatat agtattttgc cagactcata gtccattaaa actgcatgtt tagcaactat
    20461 agctggttca ttatctgcaa aagagatatt agagcttcct aaaaatgaag taaatatcaa
    20521 taaacagatt aataaaagtg ataaatttct tttcattgtg agcctccaaa ttttaaattc
    20581 atctttta
//
LOCUS       DAFKMX010000059        20134 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFKMX010000059 DAFKMX010000000
VERSION     DAFKMX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.059"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(174..1778)
                     /locus_tag="LZB08_001718"
     CDS             complement(174..1778)
                     /locus_tag="LZB08_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7315223.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2165..3091
                     /locus_tag="LZB08_001719"
     CDS             2165..3091
                     /locus_tag="LZB08_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBM7315224.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3211..4209)
                     /locus_tag="LZB08_001720"
     CDS             complement(3211..4209)
                     /locus_tag="LZB08_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBM7315225.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4313..4750
                     /locus_tag="LZB08_001721"
     CDS             4313..4750
                     /locus_tag="LZB08_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7315226.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            4836..5423
                     /locus_tag="LZB08_001722"
     CDS             4836..5423
                     /locus_tag="LZB08_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBM7315227.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5723..6343)
                     /locus_tag="LZB08_001723"
     CDS             complement(5723..6343)
                     /locus_tag="LZB08_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBM7315228.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6351..7118)
                     /locus_tag="LZB08_001724"
     CDS             complement(6351..7118)
                     /locus_tag="LZB08_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBM7315229.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7159..7980)
                     /locus_tag="LZB08_001725"
     CDS             complement(7159..7980)
                     /locus_tag="LZB08_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315230.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8339..10249)
                     /gene="phnW"
                     /locus_tag="LZB08_001726"
     CDS             complement(8339..10249)
                     /gene="phnW"
                     /locus_tag="LZB08_001726"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBM7315231.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRENGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10528..11313)
                     /locus_tag="LZB08_001727"
     CDS             complement(10528..11313)
                     /locus_tag="LZB08_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBM7315232.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11530..11760)
                     /gene="dltC"
                     /locus_tag="LZB08_001728"
     CDS             complement(11530..11760)
                     /gene="dltC"
                     /locus_tag="LZB08_001728"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBM7315233.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(11779..12927)
                     /gene="dltB"
                     /locus_tag="LZB08_001729"
     CDS             complement(11779..12927)
                     /gene="dltB"
                     /locus_tag="LZB08_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBM7315234.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     VSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMVTMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(12927..14441)
                     /locus_tag="LZB08_001730"
     CDS             complement(12927..14441)
                     /locus_tag="LZB08_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBM7315235.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14562..15752)
                     /gene="dltD"
                     /locus_tag="LZB08_001731"
     CDS             complement(14562..15752)
                     /gene="dltD"
                     /locus_tag="LZB08_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBM7315236.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16040..17059)
                     /locus_tag="LZB08_001732"
     CDS             complement(16040..17059)
                     /locus_tag="LZB08_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBM7315237.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17235..17774)
                     /locus_tag="LZB08_001733"
     CDS             complement(17235..17774)
                     /locus_tag="LZB08_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBM7315238.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(17875..18555)
                     /locus_tag="LZB08_001734"
     CDS             complement(17875..18555)
                     /locus_tag="LZB08_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBM7315239.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18584..19873)
                     /locus_tag="LZB08_001735"
     CDS             complement(18584..19873)
                     /locus_tag="LZB08_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBM7315240.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6605 a         3493 c         2062 g         7974 t
ORIGIN      
        1 atctacagtt atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa
       61 taattaaaat ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc
      121 taacttatta tataaaacta ttttttatag attataaaat atctaacttt catctacttc
      181 aaagaataca atacttctcc ttctcttata gtctgcaata ctttaatatc ttttattttc
      241 atagggtcga ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct
      301 ttaattgaac cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc
      361 tttaaagcat ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta
      421 attctattga cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc
      481 tgatgaagag tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac
      541 gctctatctt ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca
      601 aagtatgaag gtatcatatt tattactttc atatcatcaa tctggtctgc tcttactgtt
      661 tgagcatgta tcataactgg tcttatatta ttttcactac tttgctcttt taagtttaaa
      721 actttctcaa acgcatctat caactggtct gctgctgcat ctccattaca atgagttaat
      781 agttgcatct tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat
      841 ataggatatc cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc
      901 ttaccttgtg gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata
      961 tattttttta taaactccct attattatct actacagact tacttttttt caaatttaca
     1021 tatcctacaa catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat
     1081 tgattttctt ccattaaacc ttcttgtgct gttgtaatac catagcttag atatatgttc
     1141 tgtgcttttt caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct
     1201 acattaaaaa atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta
     1261 cctatatgtc caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc
     1321 aatgtatttg caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa
     1381 gatgcactat cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca
     1441 tatgaaaatc caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt
     1501 atttttttta ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta
     1561 gtagcaaaag ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct
     1621 tgtaaatcta taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct
     1681 atctttttta ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt
     1741 gtgatgatat ttccattgta ataaattttt gctttcataa aattctcctt acgttgtcta
     1801 cttttaataa atttttttca ttattttctg cttaattata ttatcttctt tataaacttt
     1861 ttctttagag tttaatcata taaaagtttt atttttaatt attgtttgcc aagaagtaaa
     1921 tactattcat tgtaaataag acaaattaat aggctttata ctaattgaaa aaatattata
     1981 agttgttaaa tttaaatatc taaactcaat tatatctgta caattgtaat tttttaaatc
     2041 atgatgtatt attttaaata tacagttaca ttattattca caatataagt atcttagttc
     2101 tcaaataaca tctcatgtaa tctattcaat gcaaaaaatt taaatatttt ttaggaggtt
     2161 ttttatgagc aaggaaaata aaaatgtaag aattgctgtt gtccaggcgt caccagtcat
     2221 aatggattta gaaaaaactg ttgaaaaagc attaggatta attaaagaag ctggtagaaa
     2281 aggggcaaat atcgtagttt tccctgaagc attcatacct gcatatccaa gaggtctttc
     2341 atttggattt gttgttggaa gtagaacaat ggaaggtaga gaagattgga aaagatacta
     2401 tgataattct gtaccagttc ctagtgctac aacagattta ttaggaaaag cagcacaaga
     2461 agcaggagta tatctatcaa tgggtataac agaaagagat ggtaatgata taaattgtac
     2521 cctttattgc acaaacttat ttttctctcc agaaggtaag ctaattggta aacacagaaa
     2581 acttaaacct acaggaacag aaagatgtat ttggggagaa ggagatggaa gtactcttac
     2641 agtagttgat actccatatg gaaaaatggg ttcgttgatt tgttgggaaa attatatgcc
     2701 attagctaga acagctcttt atgctaaggg tgtaaaactt tacatagccc ctactgcaga
     2761 ttcaagagaa gagtggcaag caactatgaa acatattgct ttagaaggaa gatgttttgt
     2821 aattggatgc aatcaatatg ttgaaaaaaa tatgtatcca actgacctta actactataa
     2881 agagctagat gcagaaccag aaattatgtg tccaggagga agttgtatag ttgacccttt
     2941 tggaaaatat gtagcaggtc ctattttcaa taaagaagaa atgctaatag ctgatttaga
     3001 cctagaaaaa atagtactta gtagactaga ttttgattca gaaggtcact attcaagacc
     3061 agatgtattt gaattaattg ttcatgaata atctttttac acaaacatct acataaatct
     3121 ttttacataa aaaaatgaga tactcataag taaatttcaa gtatctcatt tttgcttaaa
     3181 acctataatc ctaattataa atttattcac ctatattttt tctaaatcaa ctactgttct
     3241 tccaacatgt tttcctgcca ttattgaatc tatactatca tttaactctt ccaatgatac
     3301 tacatttacc atatcatcta aatcatctaa tttccattca tttgataata aatcccatat
     3361 ttttaatctt aagttcattg ggcaatccac agaatctata ccatataaag taattccctt
     3421 taatataaat ggatatactg aacttgtaaa atcaactcct cctgcattac cacaagttgt
     3481 tactgctcca caataattag ttgtctttat tgctgttgct agagtatttc ctcctactgt
     3541 atctattact ccagcccatc ttggtctaag cagaggtctt cctgagttat catccacttc
     3601 tgacctatgt ataacatctt taacacctaa gtttagtaat acttcttttt gttcttctaa
     3661 ttttcctgtt gaaactacaa ctgaataacc taatttaatc aatattttag ttgcaattac
     3721 agcaactcct ccagtaccac cagtgactaa tacatctccc atattcttgt ctacagaatc
     3781 tataaattta tacacagata aagctgctgt aaaccctgct gtaccataat ccattgcttc
     3841 ttttagtgaa agatttttag gaagtttaac tacccaacta gaaggaactc ttatgtattc
     3901 tgaaaaacca ccagaagtat tcattcccaa gtcataacct gttactaaaa cttcttctcc
     3961 aattttaaaa tcatcacaac tactagattc tactatacca gatgcatcaa ttcctggtgt
     4021 atgaggatac tttctcgtaa cacccttatt accatttgca gaaagtgcat ccttgtaatt
     4081 taaagaagaa tattttactt ttattagaac ttcgccttct ggcaatgtat ctacttctct
     4141 ttcaactata tttcttctaa aatttccttc ttcttctgta atatatagtg atttaaattt
     4201 tttattcata aatttcctcc caatacattt agttttatgt taaactaatt atagtttata
     4261 aatagtttta tgtcaaacta atttaaatag attaaagatt ggagaataat ttatggaaga
     4321 taaatatatt gtacatttca taagtaaaac aaaggctagt atgattaaat tcattgaaaa
     4381 taaactcagt aaaaatggtt taggtgagtt aatacctact catggaaata ttttaactgc
     4441 actttatgaa aataatggaa tacttacaat gaaagaaatt gcagctaaaa taggaaaaga
     4501 taaatccact gttacagtgt tggttaataa acttataaac ctaggatatt tagaaagaca
     4561 aaaatgtaca aatgacaaac gtatcacgta tataaagctt acaaaaaagg cactgcttat
     4621 agaagatact tttaatagca tctcaacaca agttaaagag acggcatatc ataatattac
     4681 agaaaatgaa aaacaagaat ttcttagaat tttaaaaaaa attaatgata attttaaaaa
     4741 tgctgactaa attgagcata attatatctc ctattggaat aataaataaa aagaatcaaa
     4801 tattgtaaaa atttattgtg atagagaggt agaatatgaa tttaaaagat agtaaaacta
     4861 aagaaaattt attaagagct tttgcaggtg agagtcaagc tagaaataga tacaatattt
     4921 ctgcatctgt agcaaaaaaa gaaggattat atataataga gtctttattt aattacactg
     4981 ctgaccaaga aaaatcacat gcaaaacaat tcttttctaa attaaaagaa tttgctggtg
     5041 aaaatataag tatatcagga gcataccctg tagatttata tgacaagaca gcacaacacc
     5101 ttaaagctgc acaacataat gagcttcaag agtgggatga tgtatataaa aactttggaa
     5161 aaacagcacg tgaagaagga tttgaatcta tagctaagtt atttgaaaat atagctgaaa
     5221 tagaaaaagt tcatggtgat agatttggta gatatgctac tgatttagaa gaaggtactt
     5281 tattcagaag aaatgaagat atacaatgga tatgtacaca ttgtggccat atacatatag
     5341 gtaaagaagc tccaaaagct tgtcctgtat gtcttcatcc acaaggatat ttcttagatt
     5401 ttaaaaactc attatttgaa taaattaagg caataattct atttaaacaa tttactatat
     5461 ttatatcaag tatttttata aatactgtat tatcttaata acaataaaat gaaccattga
     5521 tattactata agtagaaaat gtagtttaaa tacaatttat agtagttcat catggttcat
     5581 tttattattt actaatccct ataacctact taaatctatc tttaactttt acacactcac
     5641 ataaaatctc atctttaaac taaatattta aataaagtgt attttaagta tattattata
     5701 atacattata taagataata cactatgata actttatact tatatcattt atttcttttg
     5761 tcaagtcaac ctcaataatc ttaactacat ctttttcaaa ctttactttt ttataatcca
     5821 aacatcctac tacgaatgga actccaaact ttctaaacaa ataaatcttt ccactttcat
     5881 tgtatattga tgtaaaattt tcttctatta tattcactat caattttaac tcttcaaaag
     5941 ttattataga tttaactaaa aacttctcta ttatgctcaa tataagcagt tcttttttac
     6001 ctatgctttg ctctttttta tataaatttt gaaatatttc tttggtataa ttacatataa
     6061 cttctttact gagaataaaa tcaatgtcaa attctttatt aatatccttt ttcataaaca
     6121 tatttgcaat atcatcaaga gaagtctctt ctttcataga caatattaac tctattcttt
     6181 ctagtatttt atctcttgga aagaaagtct cttgacctgt aaaagctgat ttttttataa
     6241 accattcctc tggtattatg ttttttcttt tccatctata taattgacca taagatatat
     6301 tagttttgct caataaatct ttcttagata ttaattcttc cataactttt ctagcccacc
     6361 tttatactgt tttttacagt agaattatct gaaactgata aattatcact gtattcaata
     6421 tttccaacgt cacaattatc tactagagat atgcttttgc ctcgtatatc ttttattgat
     6481 gcatcttcta attcaatagt atctccctct atcaaattac atttaaactt tcctgaaaaa
     6541 atattaatga ttttaattaa tgaaaaagca tctttgcttc ttcctatttt tatattagtt
     6601 gcccctatct ctttacaata agaacctcct cctgaataaa catatatatt ttctccattt
     6661 aaaagaccat cacattttat tgctccatat acctttatat cttcacattc tatatttgat
     6721 ttatttctca tttcgccata gatttttata tcttgtgcat acatattttc atttacactt
     6781 aattgtcctc ttattgtaac attattacat ttaagtttgc catccacttt ctgttgagca
     6841 gttataacta agtcatctat ttctgcatct ccctctatat taagttgacc attcactctt
     6901 acacgctctg ctttaagatt gcctcttata atagtttttc cgttaacaac aaaatttttt
     6961 atattgatgt caccttcaaa ttttgcatct ccaaggactt ttacttcgtt acttttaaca
     7021 tttccttttg atttagaact tcccacgatt tttatatttt catactcacc actacttata
     7081 aagccttctc caagtatttt tatagaccct ttttccatat tattacctca ttttaaattt
     7141 tatttttaaa cctatttatt acttatcctt ctttaatttt attttttcac ttatattctt
     7201 tatttcacca actattgaat cttcatcaat ctctatactt tcactgtact ccaaactttc
     7261 tacataacat cccttgccta ttttaatatt ttttcctctt acaactttga cttcactatt
     7321 ttctaagctt atttcatctg cttctataat atttgcaaac aatttacttt ctgtatactt
     7381 tctaattgat aatagaccta aacttattaa atttgaagac actttttcaa agttgatatg
     7441 ctttttttct agcatacgaa attttttgca ataatctcca tgcccctttt ttatatttat
     7501 actacttgct ccaacttcat taaaattaga agttccattt aaactaatat taacttcttc
     7561 acaattcaaa aatccattac actccaagat tccacttaaa ttgatatttt cacaatctat
     7621 attctcattt gcttttaaat atccttctcc atgaataata tccgatttta tacttccttc
     7681 tacattaata attccactag tatcaatttc aacagaatta atatttcctg tacatctaag
     7741 aagtcccgat atctcaactt ttgatgccag tatgtttcct tctactttac ttaaaccaga
     7801 tgtaaaaaat tcattacatt ctgttgtccc aaaaactttt gctgaaccag aagaatcaaa
     7861 tttactacat tttacacttc cttctatttt gcctgttcct gatatattaa cattatcaaa
     7921 aattccacct ttacattttc caacaccatc aaatcttagt gaacttaatt cttcattcat
     7981 aatgatatcc tttctttacc gtattttatc tttaaaccta atataacaaa acattgttac
     8041 gttgtcaagt atgataataa tacttaataa agcaactatc atttattttc tataaatagg
     8101 cataaaaata atagggaaat tctttattta aactataaat ctaatttctt agattgtggt
     8161 ttaaataaaa tatatcccta ttactcaatc tttatatatt cattgtataa acttctaaaa
     8221 tataagtctt taaattctac tgtcaatact ttttatacac tgtttgtgct atcatctcac
     8281 acacctaaag gagtgggctt tccgcactat tctgtaacct acaagacact atcttcttct
     8341 atctattgat aaatttttct attacatcag ctagtctctc catatcagtt ggataaactt
     8401 cacctatgct acctattcta aatgtatcta tatcagtcaa tttacctgga tagataacaa
     8461 atccattatc ttttaaataa gtataaaatt ccataaattc aaactttgca tttttaggat
     8521 ataagaaagt tgttattaca ggtgattgag cattatcatt caccaaagta tcaaatccta
     8581 aagattttaa tcttgaagct attgtaaact gattttcttt atatctagca tatctttttt
     8641 caacagaacc ttcttcttcc aattctaata aagcttgata aaaagctctt acaacatgtg
     8701 taggtgaggt aaatctccat ttaccattat ttttctccat agtctcccat tgcgcataca
     8761 catcaagaga taatgattta gctatacctt tacatttaga taatttctct ttattagcta
     8821 ttataaaacc aaatccaggt actccttgta tacatttgtt agaactacta actaaaaaat
     8881 ctatattaaa atcttcaaca tcaatctcaa ttcccccaaa actactcata gcatcaacta
     8941 tataaatttt gttatattct tttgcaagtt tccctacctc ttgtatagga tttaatctac
     9001 ctgtagtagt ttcacagtga accattgata tatgtgttat atccttgttt tcttttaata
     9061 aattttctac tacatttaaa tccactgctt ctatatcttt aaatgtgcaa tcaacaaatt
     9121 ctatgttaag ataattacat atatctttca ttctctttcc atatgctcca tttgcaatta
     9181 caagaagttt accatctttt gaaatagtac ttcctattat agcttctact gaaaaagtcc
     9241 cacttccttg cattaataca cttgtgtatt tatctgtatt ttgggttgct agagatacta
     9301 atcttcttct aacatcttgt actaggttat tatattcaac atcccaagta caccaatctt
     9361 ttaacataga tgctcttact gatttagtag tagatagtgg tcctggtgta agtaatatgt
     9421 aatcattttc tggtacaaaa tctgatttaa tagtttcatt ctctattttt attagtatat
     9481 cttctaactc agccatagtt tctattacaa aatgtgcacc tgcctcttta aatttctttg
     9541 aaactatact catcttagct tttaattctt ccttatccat gttctctact tcttcttgtg
     9601 taagccctaa ctcacttgaa ccttttatta cagcaacact ccacattcct gcatttacgc
     9661 cttcttttac atcacttatt gtatctccta cttttaccat agaactcata ggagacaccc
     9721 ctaaagcttc agcattttta tagcacatcc aaggatacgg tcttccttga gatacttctg
     9781 atggtgttac aagaaaatcg ggtgagtatc ctttctttgc tgcatttggt tcaactatgt
     9841 tcatcatctc tcttgtataa cccgttgtag aacctatttt taatccattt tctctcaact
     9901 tttcaatagt ttcaacaaca tgaggaattg gagtcgtata ctcttctaat gtctcaaata
     9961 acattggctc aaattcagca taaagttcat ttacatcatc ttcagttggc actttaccaa
    10021 acttatctga ccaaatattc tttattctat ccatttcaca catttctctt atatggtcta
    10081 tttttaattt tcccataggt tttcttgctt cttccattgt tacatctata cctcttcttt
    10141 taaatatctc tataaataca ttaagaggtg caaaacatcc ataatctact gttgttccag
    10201 cccaatcgaa tactactgct tttatttttt ctccatatat ctttttcata tttatagcct
    10261 ccatagattc tttttagaat ttttgtgttt ttaattgttt ttacttttaa agtataactt
    10321 tcatatgtta attatacatc atatttaggt taaatatata ttaaatttct gtttttagtt
    10381 gtatttagtt gtttttattt tgtatcacat tcctataaaa tcctaagtta caatctaaaa
    10441 tttactttct aatcttaatc tatgaactga ctatatttta tgaatttagt tgtattgagc
    10501 atagttgtat tatataaaac taagggttta ttttgcttct atccatgtta tatttatctc
    10561 atttaactta tccttccatt cattcggagg ttctacatca caaataacaa aatctaattc
    10621 ctctaaatct gctatcttat acatactagc aattcctatt ttagaactat cacataatac
    10681 aaaactttcc tttgaatttt ttatatattc ttttgacaac aatgccttat tttggtctat
    10741 actactaatt ccaaaactat caagtatacc ttcactagat ataaaagctt tatctacgta
    10801 taagtttttc atagtatcta ttgacattgg acatgctgtc ctttggtgtt ttaaatttac
    10861 ttttccacca ataaatataa cttctccatc aaacaaacct tgattctcat aagaaatcaa
    10921 ttttgataga actggataag agtttgtaat aatttttaaa ttgtttacag atattatata
    10981 atctacaatc tgcaaattgg tagtaccttc atctatgata attgactcat tgtcatttac
    11041 aaacttagct gcatatttag caatactttg ctttttatct aggttagtat tgtttctctt
    11101 tatatgttct ggttcaatca ctccataatt tatttttata gcaccaccat ataccttttt
    11161 taatttattt tcattttcta attcttccaa atatcttctt attgtttctg atgatacttc
    11221 taatcttttt actaattctt tagtgtttac cttccctttt gaatctaata atttaagtat
    11281 ttcttttttt ctatctatcg aatttaaaga cattcatttt cctcctaaat ataatatatt
    11341 tttctcctat aatattacaa tgatacctaa gatttatgaa atcatattta tgtattaaat
    11401 tttttactta attaaagtta ttttcaaaag actttgtatt atcattaaaa aagtccatca
    11461 aatattagta tcagatggac tttgtaaaat tttattttat aaattaatat ttaaagtcta
    11521 actactaatt tattttcttg tctccaagaa ttttactaaa ttatttacag tagacatatc
    11581 ttttctctct aaatcagttg gttgaagctc tatccctaat ctattttcaa tttcaagtaa
    11641 aacttcaatt atagctaatg agtctaaaag ttccgtctca aataaattaa ggtctaaatc
    11701 ttctcttatc tcatcagtcc ctaatacatc ttcaaatatt tctataactg tttcttgcat
    11761 aaattttcct ccttaaaatt aagctaaata acctgagaat atcagcaatc caaaacacac
    11821 aatatggaat gtaatgaaaa tctgcactct ctcgaaccat ttatcttttt tatgtttttt
    11881 ataaaattta gattttcgct gatatatatc agttaacaca agtgccaatc cttggtatac
    11941 accataagca acataatacc aagttagacc atgccaaaat cccattgtta tcatggttac
    12001 catctgagcc acatgagcag cagtagtcct tttcttaaat cttttctttc tcattgatga
    12061 cataacaaat ctagaaaaaa tataatctcc aaaccatctt gaaagactta tatgccatct
    12121 tgtccagaac tctttcatat ctttactgat aaatggcttg tcaaagttta taggtacttg
    12181 tattccaaat atgtaccctg tacctatagc aaataaacta tatcccgcaa aatcaaagaa
    12241 caggtataaa ctatatgcgt acatataact tacaatattt ataaaactca tatctttagg
    12301 tattcttgat acccaatatg tatttatcaa aaatgctata acaaacttat aacctactcc
    12361 catgacaata ttttttattc ctggcagtaa gtattcttct atatactctt ttctagagat
    12421 ttgtttttcc aagtcctgtt caaatcttct tgacctatca ataggtccag aacttaaagt
    12481 tggaaaaaat aacataaaat aaagcatctt tgaaatttta acttctttta tagctccatc
    12541 ataaatttca ataaccattt gtatagttct gaaatttaag tatgatattc caataaatcc
    12601 aattattcca aaagaagtaa ctggagatat tttatttatt attattggga gcattgatgc
    12661 aaacaagaat ccccaatata tgtatttatt tttagttttt cttcttacat attcgtatcc
    12721 tttaactatt attacttcaa gaataataaa tattaccaag tactttagtt ggacatcaat
    12781 tcctactatt aagaatatca taaacaaact tgccaacata ccataatact ttatattctt
    12841 gcccattaga cctaaaatta ctgctggtat tgatgttaat aataatatat aaagataaaa
    12901 ataatctcca tattgtgaaa aaatcattaa atttcctcca taagtttctt tctgtctatt
    12961 ttaccattga tatttgttgg aaactcagag attattgaaa tatttcttgg aatcatatat
    13021 gaaggtatat actttccaag ttcctttttt attatcatcc catttttaat attacttaaa
    13081 tcattctttt catttaactc aacaatacct tttaagtaag caattttttc gtccttatat
    13141 actggtaaaa ctactgcatt ttttacatta tgtacttttc tcaaattatt ttctatatct
    13201 tctatttcaa ttctaaatcc atttaattta atctgaaaat cttttcttcc acaataatat
    13261 atatttccat ctaaaagata tcccatatct ccagttttat aagctctcca ctttacacca
    13321 tctatttcat catagaaaaa tacttcatca gtcttttctt tattattgaa gtaaccttta
    13381 gaaactgatg gtccaattat tataatttca cccttttcat tttctttaag ttcatttcca
    13441 tcttcatcaa gtatttttat tttacaattt gacatagggt atccaactgg aagacttttt
    13501 tcatcatcta tagccttttg agtcatatca tttacactta caccaacagt agcctcggtt
    13561 ggtccatatc cattgataat tcttgtgttt ggaaatctac tcataagttc tttagtaaga
    13621 ttttttgaaa gtgcttctcc tatgaatatc attgactcaa ggttaggaag cattttacta
    13681 ttaaactctt tttcagttat acatactcct gcaaaagaag gagttgacac ccatacagct
    13741 atatctgaga tactaaattg tctaaatagt tctttatagt ctgctaatac atcttttgat
    13801 attgaaaata atgtagctcc atggataaga ccagggtata ttgttgtaac tgaaaggtca
    13861 aaagaatatg ctggttggtt cattattact ttttcacttc catctatgtt tagatatggg
    13921 cttatccaat ctgaaaagct atctaaattg tttgaactta tttgaacccc ttttggcttt
    13981 cctgtacttc ctgaagtaaa tagtatatat gcattttcat catcttttac ccagttttct
    14041 ttatctaatg attttccctg atattcattt attatataat ttaatttatc catatcaatt
    14101 acattgatat ctccaaaatt tctttcatca ctaaagttaa ataatacctt tggttttatc
    14161 tctttagtga cttcaaaaac tctatcaatt ggaaaactaa tatcaagagg cacatacgct
    14221 ctacctgatt ttaaagctcc tatcatacat gccattatca tgttttcttt atttccataa
    14281 attactatag gagtatcttc ttctttgtat acatccttta gaaatactga tatagcatct
    14341 gaatattcat ttaaatcttt ataagaaagt ttatctccat tacacattag tgctgttcta
    14401 tctgtatttg aatatttttt tattccttca ataattttca tataagaatg ctcctttaaa
    14461 atccgttata aataaatggc aattctttaa atggagtaaa tgttagaact cctatcgcca
    14521 atattataac catagttaaa caaatactag ttaacaaatc tttattgttc tttaaatatt
    14581 ttaaataatc tctcacagac atctacccat cctttcgtac caagatgcat tacatccctt
    14641 aaataatact tatctgaccc tttatcttta aggtttagaa cttcaaagcc tttgctttct
    14701 gctatttttt gtgctttatc gtagtactga tttctttctt tttcagaaat accagtcaaa
    14761 ttatagaatt tatccatact tgggattaat acaacaactg gcttaatacc taagtcagta
    14821 catacattta gcatcagttt ataattttca aactccttag atgttaataa atttacatct
    14881 ttgtctcttc ctttgtattg ctctatacca tctttaaaat gctctttata atagtactta
    14941 tcaatattta gtggattttt accaactctt tttttagcat cttcaatggc tttctttctc
    15001 tcatatgacc aatttattgg tctttttctt tttgttgctc tctttttatc tagcttaatt
    15061 aatcgtttat aaagaatacc cttttcttta agagctatac agtactttct tccttgaaaa
    15121 taaggctcta aaagcacctt ttcagctttt tcaggaaatg tcttaggctc atatagctta
    15181 gcatataatg cttctgcttt atactcgtca cttccccata ataacttact tgattttttt
    15241 gcatattcaa tcttattttg tttagatatt tttggattat ctaaaaatcg ataaaattgt
    15301 attggtgaaa atctagactg ataatggtga cttgtgactc cgtctttttc cataaaccat
    15361 tgcatagaaa ttaacaagac aactttctta ttatcaatat taggattcat acttcctaat
    15421 attgcagcat cttgtaatgt ctgagtatag gctctcccaa ttgcaaacac cttattttta
    15481 ctcctatttg tattaaaata gtactttgga tgttgttttg ttgaattact aagttctgat
    15541 gaccccaaaa tcataatatc cttttctctt aaaaaatgat tatttgctgt aaccccttta
    15601 tcttttatat cacttaaagt atcatccata attggaagta gatttttctc cctcaaaagt
    15661 tcatcagttt tagaatcaag aaatttgtct aacccaaata aaaatactac gcctattata
    15721 aaaggtgtaa taaagtatat caattttctc atattttctc tcctctaaaa atattcaaat
    15781 ttatgttatt attaatcata ttagttttga tatgtatatt caactacaaa attgtaataa
    15841 aatcgtcaaa actttttttg atactgttac aaaattgtaa aaaaaaagaa aaaaacgcca
    15901 aaaataaatt tctttttagc actttttcat acttataatt tgacatttta acacttggct
    15961 aagtatacac cttaccttaa cttaaaatta aatttattta agaatatata ttataaattc
    16021 ttatattttc taatataaac tatatactat cagctggata aacaatacta ctttgtcttc
    16081 ttatctcatc cataatttcc attactatta ttgaattttc atgagaattt atatttgatt
    16141 ctattttatt gttatttata agacttataa actctacaag ttcataatac attccttcta
    16201 tttctctagg cttttcctct gctttttctt ttggaataga tatatcttct tctcttccat
    16261 ctctgtattt aataatagct ttttcaaata gattaagctt ttcaattatt atacttccct
    16321 cttctccttg tatttcagat ggtatataag aatcagctac tttagaatac tgaattatcg
    16381 catccatatc atcatattga aatacagctg aaccttctcc atccactcca gtttgaagta
    16441 tatgagaaac tgcctttaca gtttttggag cgccaaataa attcaccata ggatgtatac
    16501 aatacactcc tatatccatt aaagctccat ttgataactc ttttttaaag gcattttcta
    16561 taattccatt tttatattta tcatatcttg aagaatactg acaatagctt ccaaaaaatc
    16621 ttcttatttt ccctatttta tataagtttt ctttaactgc tttaaagttt ggaacacatg
    16681 taattctcat agcttccata agcaatacat tattatcttt ggctgttttt atcattgatt
    16741 tgacttcttt taaattagat gctaacgact tttcacaaag cacatgtttt ttgtgtttta
    16801 aacaagtaat ggtttgcttt gaatgtaatg catttggaga agctatgtat acagcatcta
    16861 tatccttcga tttagccatt tgttctaaat catcaaaaaa aatacttgct ccatatagag
    16921 ttccaaattc tctagctttt tctaaatttc tagaatatac agcaaccaat tcaaagtctt
    16981 taaccctaga tgcagctctt agaaatatat ttgctatatt gcttgtccca acaataccaa
    17041 atcttatttt acccatcata aatcactccc cattttctac actttatttt aattttaata
    17101 aaaaacttta caacttatag atatattata aaataatttt atcaaataaa aaagaaagct
    17161 aattttaaat gatttcacca ttttaacata gctttctttt aactacaatc taaattcaac
    17221 ttatattttt aaagttatct ttctactata tctttatttc ccctcaaaaa ttttatagta
    17281 agaatttcat ccacaaacaa tgttactaaa agtatagtta caacaattgg ccaataaggt
    17341 attataagac tatataaact atcaactata tacacttgaa aatatctaac tccaataaat
    17401 gtcaatatag tccaatatac agaaaacttt agacatacta ttcctctact attgtactta
    17461 taatctgaat aatcccaata gaccttatta aaatatactt tcataagtat tcctgtaata
    17521 tattctacta ctgtcggcac tactagacat aataaaagca atgcaaatat attattgggg
    17581 tcaataaatc ttgagacctc tattataatc gacattgcaa ttgcatacat tggtttaaat
    17641 ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt tataataact atataagttt
    17701 tcaattatcc aacctataaa accatacaag aaaaaattaa ataaaaaata taatagagaa
    17761 cctacattat ccatgaatat tcctcctagc tgtttatact tattttttct catattcatg
    17821 gatttattct aaatcaaaga aattatattt aatttattta attaatactt tatattatag
    17881 attgtgacat tctttagagt tcaaactttt taggtattct ctaacttcca gtaaattttc
    17941 aagtatctta tgtgattgtg atttaatttg ctcatctggc tcttttaaat ctggtacatt
    18001 tatacatctt ataccaccat tgtatgcagc ttcaactccc ataggcgaat cctctatgac
    18061 aatacaattt ttgggattaa catttatctt tttagctgct tttaggaata tctcagggtt
    18121 tggtttcgag tttacaacat catctccaca aactatagca tcaaagtaat cttttaaatt
    18181 tgccttagct aatctcttta ctgctctttc tctctttgtt gatgttgcaa cagccatttt
    18241 gtatccattt tcctttaaaa aagagattag ttcattgact cctaatttga taggagctcc
    18301 cttcctctcc ataaattcaa tcatattttt agttttttcg tcatataaat ctattattgg
    18361 tacagaacta tcgtatatat ctgtaagacc ttctattatt cctttacgat ttcttcccat
    18421 aactgatgta taaatttcct tagtcatagt atatccatac ttttcaaatg tctccatcca
    18481 aaattcaaga gaaattctct ctgagtcaaa caaaactcca tccatgtcaa aaataattcc
    18541 ttctactttt tgcatacaat tcctccaata ttttttgata taattatatt acacttttta
    18601 tgattaattt tacaaccctc aatttatcca attctaatat tgtaaaattg taattttcaa
    18661 aagttacact ttggtttact ttaatatttg aaccaagcat ataggatatc catccaccta
    18721 tactgtcaaa tccatcatgc tctatatcaa gtccaaaata cttattaata tagttgatag
    18781 attcagttcc atcaactaag taagaacctg ctcctatttt cttaatatga gtctcttctt
    18841 tatcaaattc atcttgaatt tctccaacta tctcttctag tatatcttct attgttacca
    18901 caccagatgt accaccatat tcatccacaa ctatagcaag ttgaagtttt tcctttctta
    18961 ttctctcaag agctttatct atagttaaat tttcagatat atatataata tctctcaata
    19021 tttcttctag ttcaatttta ttttcattta tcttttgatt gtataaatct cttatatgta
    19081 caaaacctaa tatatcatct ttattttttc tgcatactgg atatcttgta acaccttcct
    19141 cttttaaaat agctaatatc ttttcttcag aatcactttc atatatacat accatatcag
    19201 ttctaggaac cattatctct ctaattttct tttcttcaaa ttcaaaaata ttatcaacta
    19261 atctttgctc tgattcatct ataagtccac ttttataact ttcttctact aatagtctta
    19321 tttcgtcacc tgtatggggt tcatcaacct catcagcttg tgaatatcca aatggtttaa
    19381 gaaataaatc agtacttaag ttgaaaaaat atattattgg atatgttaac ttataaaatc
    19441 ctactaataa aaaagatgta ctcaacatta ttttttctgt attataaagt gctaaagctt
    19501 taggaaccaa ctctcctatt acaacctcca tcattgttat aattaaaaaa gaaatggaaa
    19561 ttgatatagt atatataata ctttcactaa gattaaatac acttactatt ggtaaaatta
    19621 actctttaac tgtagattct cccatccaac ctaatgcaag tgaacaaagt gttataccaa
    19681 gttgacatgc agaaagatag gaatttaagt tgtcttttat tattttacat ctttttgcat
    19741 tattattacc ttcagttatc atggtatcaa tcctagaact acgaagtttt accatagcaa
    19801 attcacaagc tacaaaaaaa gcatttatca aaacaagcat tactataagt gttactttta
    19861 ataactcccg cataaagtat tggttcttat aatgtaagaa atctataaga actcaaagtt
    19921 ccccccttat atttataaat ttaatttata atatatattc tatcatattt attaatatat
    19981 cctactttat tacaaataat ttttataaac taaataaccc cttttaatat ttatgactgg
    20041 ctatttgata aatattaaag gggttattta gttatcattt tttatttcta aaaatctttt
    20101 ctcaaatgat ttagttatct atttatccaa tttt
//
LOCUS       DAFKMX010000060        19742 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFKMX010000060 DAFKMX010000000
VERSION     DAFKMX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.060"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            135..485
                     /locus_tag="LZB08_001736"
     CDS             135..485
                     /locus_tag="LZB08_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBM7315241.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            504..2147
                     /locus_tag="LZB08_001737"
     CDS             504..2147
                     /locus_tag="LZB08_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBM7315242.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2228..4285
                     /gene="recG"
                     /locus_tag="LZB08_001738"
     CDS             2228..4285
                     /gene="recG"
                     /locus_tag="LZB08_001738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBM7315243.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLAKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4344..4898
                     /gene="rsmD"
                     /locus_tag="LZB08_001739"
     CDS             4344..4898
                     /gene="rsmD"
                     /locus_tag="LZB08_001739"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBM7315244.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4900..5397
                     /gene="coaD"
                     /locus_tag="LZB08_001740"
     CDS             4900..5397
                     /gene="coaD"
                     /locus_tag="LZB08_001740"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBM7315245.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5401..5925
                     /locus_tag="LZB08_001741"
     CDS             5401..5925
                     /locus_tag="LZB08_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315246.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6269..8188
                     /locus_tag="LZB08_001742"
     CDS             6269..8188
                     /locus_tag="LZB08_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7315247.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITEVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNNEVKEDVVEEYIDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8201..8488
                     /locus_tag="LZB08_001743"
     CDS             8201..8488
                     /locus_tag="LZB08_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315248.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8621..9880
                     /locus_tag="LZB08_001744"
     CDS             8621..9880
                     /locus_tag="LZB08_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBM7315249.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9883..10623
                     /locus_tag="LZB08_001745"
     CDS             9883..10623
                     /locus_tag="LZB08_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBM7315250.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10946..12256
                     /locus_tag="LZB08_001746"
     CDS             10946..12256
                     /locus_tag="LZB08_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBM7315251.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12354..13271
                     /locus_tag="LZB08_001747"
     CDS             12354..13271
                     /locus_tag="LZB08_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBM7315252.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSNTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13287..14096
                     /locus_tag="LZB08_001748"
     CDS             13287..14096
                     /locus_tag="LZB08_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBM7315253.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14154..15902
                     /locus_tag="LZB08_001749"
     CDS             14154..15902
                     /locus_tag="LZB08_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBM7315254.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKELDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16164..17186
                     /locus_tag="LZB08_001750"
     CDS             16164..17186
                     /locus_tag="LZB08_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBM7315255.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            17191..19497
                     /locus_tag="LZB08_001751"
     CDS             17191..19497
                     /locus_tag="LZB08_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBM7315256.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSERRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYVLS
                     SLSLKIFSQNIRNVRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSITYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFHMYIKNIGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEISEGIVLECDLCEGKIDERGILYRVNNIDSIMQNLFLRDEMMDWI
                     LQNKKILDSKPNEKKEKSIDEFEPMEYI"
     gene            19469..>19742
                     /locus_tag="LZB08_001752"
     CDS             19469..>19742
                     /locus_tag="LZB08_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315257.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYFILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETNSSKSDKKRYLYIYQN"
BASE COUNT         7827 a         2049 c         3636 g         6230 t
ORIGIN      
        1 aaaacttttc taggtttaaa ttataccata tttatagtat gatatgatta taattttata
       61 taataaataa gtaaaaatag taaaaaataa attaagaaat gtttttaata aaaataagct
      121 taagggggtc aaccatgagt gcaaaggtaa cgaatcaata tggaaatata gaaatagata
      181 atcaagtaat agctcaagtc gtatatagag ctgcaatgga aagttatggc ttagtaggtt
      241 tagcatataa gtctaaagga atagtagagc tattaaaagg cgagaacgca actaaaggtg
      301 taagagttga ggaagtagaa gaagatgcca tagcaatcga actttatgtt ataatacaat
      361 atggtacaaa tatatctact gttgccaata atataataga tagagtaaaa tatgtagttg
      421 aaaaaatgac tggcgtaaga gttactagaa ttgatattaa tgtacaagga ataagagtta
      481 agtaatttca taggaggaaa caaatgattc aatatataga tggtaaaagg ttaagggaga
      541 tgtttatttc aggtgcaaac aatctccaaa acaataaaga gttagttgac aagttaaatg
      601 tattccctgt accagatgga gatacaggaa ctaatatgtc attaacaata tcttatgcct
      661 taaaagaatt agcaaaagtt gaaaatgata atatatcaga tatcggaaaa gcattatcta
      721 aaggttcttt aatgggagct agaggaaact caggagttat actttcacaa ataatcagag
      781 gtatagctaa atctatagaa ggaaaaagta aattatcaac agaagattta gcaaaagctt
      841 ttaaaaatgg ttcagacaca gcatataaag ctgtaataaa accaattgaa ggaactatac
      901 ttactgtagt tagagaaagt ggagagtttg caataaaaac tgcgaaaaaa gagaaagatg
      961 tagtaaagtt tttaagtatg cttgtgaagg aatcaaattc ttcattagaa agaactccag
     1021 accttctaaa gaatctaaaa gaagctggtg tggttgactc tggtggaaag ggtcttgtac
     1081 ttatatatga aggaatgtta gcttctataa aaggcgataa catagaaata aaaaatgttg
     1141 acctagatac taatatttca acaagtatgg attttgcaaa gagtactact agcacagatg
     1201 atataaaata ttgctattgt acagaattta ttttagaaag ttctaaggta gaagatacta
     1261 aaataagaga cataatgatg gcttatggtg atagcctagc agtagttgga gatgatggag
     1321 tcataaaggt acatgtacac actaatgacc caggaaatgt acttcaagaa gctctaaagt
     1381 acggtcaatt attaactata aaaatagaaa atatgaagtt acaacatgaa aatacattac
     1441 tggatgtaga agaaaagaaa gaaaatgata gtgaaccttt agaagaagaa aaagagtttg
     1501 ggtttatagc tacttctatg ggtgaaggtt tggcaaatat atttaaagat ttcggagttg
     1561 accatataat agaaggtggt caaactatga atccaagtac tgaagatttt atgaatgcta
     1621 taaagcatat aaatgctaaa aatatattta ttttcccaaa taacagcaat ataataatgg
     1681 ctgctaatca ggcaaaagaa ttgagtgata aaaatataat agttatacct acaaaaaata
     1741 cgcctcaagg atttgctgca ttagtaactt ttaatggaga attaagcgaa gatgaaaata
     1801 aagaagctat gatgaatgct ttaaactcag ttaaatcagg tcaagtaact tttgctgtta
     1861 gggatacagt gatgaatgaa attgatgtta aagaaggtaa tataattgga attgcagaag
     1921 gaaatttatt atcagcagga gattatgtgg atgaagtaac atcaaacctt attgaaaagt
     1981 tggttgatga agatactgct ataataacat tattctttgg tgaagatgtt acagaatctc
     2041 aagcaaatga actacgtact tctttagaag aaaagtttga agatgtagat gtagagttat
     2101 attatggagg acaacctctt tattattact taatttcagt tgaataatga ttataagaac
     2161 ctgtatgacc aagtagcttt tataaaattg gttgtacagg atttttgtgt agtaggtgat
     2221 atatttgttg gatttatata aagatgttca atatgttaag ggaataggac ctaaaaaagc
     2281 agataaatta aataaactgg gtatatttac actaaaagac ctattgtatt attttccaag
     2341 acaatttgaa gatagaaata atctaaagaa aatagcacaa ttggaagatg gtgagaaagt
     2401 tacgataaaa gcagtaatat caagtataaa tacatttagc ccaaaagaag gtatgacttt
     2461 aacaaagatt gatgtaaaag atgagacagg ctcggctaaa ttagtgtttt ttaataagag
     2521 ttatataaaa aatacttttc gtccaggaga ttcaatatta gtttttggga aggttaagaa
     2581 aaaatttaat aacttagaac ttacatcatg tgaattagaa tatcttacta attcacctaa
     2641 gaatacatgt agatttatgc ctgtgtacca gcttacatac ggtgttacaa ataaagaaat
     2701 tatgagtata attagaacag tacttgaaga taaagaatta attatacaag aatatatgcc
     2761 acaaagaatt atagaaaaat ataggctatg tagtatagat tttgcagtta gaaacataca
     2821 ttcaccaagt agtaaggaat cattaaaaat agctttatat agaattgtat ttgaagaatt
     2881 acttatacta caactagggt tgtttgtatt taaaagtggc agaaataaag aagatggaat
     2941 aaagtttgaa acaagtaaag acttaaaaaa aataataagt gctttaccat ttaaactgac
     3001 taaagctcaa aacagggcat tagatgaaat tatacaagat atgaatttag agaaaataat
     3061 gaatagattg gtacaaggtg atgtagggag tggtaaaaca gtagtagctc tcttagcact
     3121 tgcaaattgt gttttaaatg gttatcaggg cgctttgatg gcacctacag agatattagc
     3181 agggcaacat tatatttcac ttactgagtc tctaaaagat tttggaataa atgtaggtct
     3241 attaataggt agtcttgcaa aaaaacaaaa ggatacagta ttggaacaaa taaagaataa
     3301 tgagattgat atattaatag gtacacatgc actaattgaa gataaggttg aatttaataa
     3361 tattggttta gttataacag atgaacaaca tagatttggt gttatgcaaa ggagcaaact
     3421 ttcattgaaa ggagcaaatc ctgatatatt agtaatgaca gcaacaccaa taccaagaac
     3481 tttagcactt atactctatg gagatttaga tatttctatt atagatgaac ttcctccagg
     3541 aagacagcca atagaaacaa ttgctattga aaaaagtaag cgagatagag catataataa
     3601 tcttgtaaga agagaggttg aatcaggaag acaagtgtac attgtatgtc ccttggtaga
     3661 agaaagtgaa gccatagaag ccaaatcagc agttgaactt gttgaggagt taagagcgga
     3721 atattttcac gacttaaggc ttgggttgct tcatggtaaa atgaagtcaa gtgaaaaaga
     3781 tgaagtaatg agacttttta aagataaaga aatagatatt ttagtttcaa caacagtaat
     3841 agaagttggt gtcaatgtgc caaatgcaac attaatgata atagaaaatg cagaaagatt
     3901 tggacttgca caattacatc agcttagagg gcgagttgga agaggtagtc ataaatcgta
     3961 ttgtgtacta atttatgatt ctaaaacaga tgtatgtaga caaagaatgg ctataatgga
     4021 agagaccaat gatggtttta agatttcaga gaaggattta gaaataagag gacctgggga
     4081 attttttggg acaaggcagc atggtcttcc agaacttaag gtagcaaact tatttaagca
     4141 tataaaaata ctgaagttag cacagcaaga agctcgatat atattgggtg aagataataa
     4201 tctacaatta aaagaaaaca tggcactcaa aaaagaaatt atagataaat ttaaagatac
     4261 attgaaagaa atttcattaa attaatttta aattgtgtta aaatattact aatgtaataa
     4321 tgtgctgaag ggagattgtc atattgagag taatttcagg aaaagcgaga ggattaaaac
     4381 taaatactcc aaaaaatgaa gatgtaaggc caactacaga tagagtaaaa gagtctctat
     4441 ttaatatgat aaattcatat ataatggaaa gtgaagttct ggatttattt gctgggactg
     4501 gttctttagg aatagagtgc ttatctaggg gtgccaagtc atgtactttt gtagatatca
     4561 gtaaggaaag catagatatt gttaagtcca atataaaaaa ggcaagagtt gaaagtgaaa
     4621 gtattatttt aaaccttgat tttaagactg caatagataa acttaaatta caaaatagta
     4681 aatttgacat aatttttatg gaccctccgt attataaaaa tatgtttata gaagctattg
     4741 aaaaaattga taattctaat ctattgaacg aagatggaat aatagttgta gaacatgata
     4801 cgaatgattt atttccagat aagatatgta aattggagaa aactagagat aagaaatatg
     4861 gaaatactac attaactttt tataagatgg aggcataata tggaaaataa acctagaaaa
     4921 gctatatttg caggaagttt tgacccaatt actaatggac atttagatat tatttgcagg
     4981 gcttcgaagt tatttgacga attacaaata ggagtattaa ataatcctaa taaaaagggt
     5041 ctatttagct ttgatgaaag agtaaaactt atagaaaaaa gtacaagcca tttaaataat
     5101 ataaaagttg tgacttttga tggtttatta ataaattact gtcaggaaaa tgggattgga
     5161 gcattagtta gaggtgtgag aagtggtgct gatgttgatt atgaacttca gatggcacat
     5221 atgaatagag agcttaatcc agatatagag actataattt taccaagttg tacaaaatat
     5281 tcatttataa gttcatcttt aataaaagag gttttgctct ttgatgctga tataaaaaat
     5341 ttggttccaa agattgtttt ggaagaatta aagaaaaaaa ctagtggggg taattgatat
     5401 atgaagatag atttagaaat gattgaacta tttgagcagt tagaggagat atttagaagt
     5461 gcttctacaa tccctttctc acataaatgc acagtggata aagatgaggt tttagcaata
     5521 attaatgata taagaacttt aataccagaa gaagtaacac aggcagtatg gattaataaa
     5581 gaaagaaata aaataataag tcaagctaag caagatgcta caaatatagt tgaacaagcg
     5641 aaaaaagaag ctgagagaat acaacaagaa tatcaagata atatagagga attaaagaaa
     5701 aattctgaag atgtagtaaa ggcatatgta gaatccagcg aacctgttgt tcaagcagac
     5761 caaagagcaa aagatatagt tgatagagct gagagaatag ccaatgagat aagaattggg
     5821 tcaattgaat atgcagagga tgtgctttct agtgttgagt ataatttaag agaaatatta
     5881 gaagaagtaa aaagagataa agctgaactt agacctaaaa aataaatttt aaataaataa
     5941 aaatggagct atcacacaaa aaattccaat ttatgtgtga tagctctctt ttaatatagc
     6001 aattttttgt tatataattg tgctttttag attacttata tcttattttg atattataat
     6061 gtagtttaat cttttatttg ttgaaatcaa cttatttatt tgtataaata atataggtta
     6121 ttttcaaatt tttttaataa ttttaccact gtcttctttt agattagagt tagactgtgc
     6181 aaaattataa aataatacta aatcatgtgc aattatttgg tatggcacta catgtagaaa
     6241 atatagataa taaaattaag gaggtagtat gaaggaaagg gctaaaaaaa tattagattt
     6301 tattattaaa aataacaatg taacatcaca agaactacaa gaaaagttta atgtttcaaa
     6361 gagaactatt tattacgata ttctagctat aaatgaacaa ctaggaaaaa gtggtaatat
     6421 aaaaaatgta aaacacaaat ttatatttga aggtaattta tgtgatgcaa ggaaaataat
     6481 ttcaactgaa gaagataaat ttttagatag tgattacaga aaaactttta tattaaacaa
     6541 gatattactt ggcgaaaaaa tatcaataga aaaactaaca aatgaaatgt tactttcaaa
     6601 aaacacagta gtacaaacaa taactgaagt aaaaaaatac cttcaaacaa tggggttaag
     6661 attagagtat aagggaaagt acaaaattat aggtgatgaa tacgttatta gagagctgtt
     6721 tctgataatt gttcaagaaa atgtactgga aattaattct ataagtgagg aagtaagtag
     6781 tttcgataca aaaggacata taaaacttac tgattattct ttattaaatc taactaagtt
     6841 tgtggaattt ctaaataaaa ggataagaga tgggaaaaca ttatactcat ataaatatct
     6901 aaatgaagct aaaaaaatta gttactttag taattgtaaa gaacttttat gtgaagaagc
     6961 taatgaaaat gaacaagcct atatatgcac ttacataagt tcacttccca gcttaaataa
     7021 tgaagttaag gaagatgttg ttgaagaata tatagataaa cttatagata agtttgaagt
     7081 aaatacagca ataaaattag aaagtaagca tgagtttaag aaaaatattt taaggcacct
     7141 ccactcttct tacaacagaa tacgatttaa gtttcctatt agaaatccaa tgttagatga
     7201 gactaagtat aaacatgagt cactttataa aattataaaa tcaattatag agaatgaaga
     7261 agagtttcct gtatttgagg ggataagaga agaagaaata ggatttatag cagcgtattt
     7321 tggaggatat ttaagagggt ctagggataa tggtctaaga agaaataaag tcttactagt
     7381 ttgtcctaat ggtcttatgg tatcaaagag tttggaaata caactatata aatacatacc
     7441 aaccatagaa atagtaggta tagtatccat taagcaacta aaagaagtaa atgtatacta
     7501 cgattacata attacaacta tagatataca aaatgtaaac aacgtaattg ttgtaaatcc
     7561 tcttttaaca tcatcagatg ttcaattact tatgaacaaa ctaataagtg taaaagaaaa
     7621 cgagaagtat ttcaatcttg aattaataat acaagctata agaaaaaatg gagttatcaa
     7681 taatgaagaa gcactaaaag ctgatttatt aaacataata cacaagatag atgaaggaga
     7741 gatgtatcaa ccaatgttaa aggaattaat taatgcagaa agggtcaata taataaaaaa
     7801 tgttagagat tggaaagaag caataaaaat agcctcaaag ccattgttag aagataactc
     7861 tatagaagag ttatatatag aaaatatgat aaagtcagtt gagaaatatg gaccatatat
     7921 tgttttagct gatagatttg ctctaccaca tgcaagttct aaagaaggag taaacaagct
     7981 tgctatgtct ttactaatag tagaagatga agttgattta ttaggaaaac cagtaaatat
     8041 atttatggta ttagctgctg tagataacac tactcacatt agagcattag catcactatc
     8101 agagatgatg tatgaggaag aaaatgtaaa actaataatt aatggtgata aaagttcaat
     8161 actagaactt ataaataagc aaaattagga ggaatgaaaa atgaagatat tagcagtatg
     8221 tggttttggt gtaggttcat caatggtatt aaaaatgaca atccaaaagg ctctaaagca
     8281 gttaggtata gaatgtgaag tagaaaatac agatataaat tcagctagag gtgctgattg
     8341 tgactgtata ttcacaagtc aagaattgtt aaatgaatta aaaggaactt gtaatgtaga
     8401 tgtatatcca gttaaaaagt acatggattt agcagaagtc acagaagttg ttcaaacttt
     8461 tttaagtgat aaaaatgata agaaatagta taagttttaa aaagtaaatt taagttaatc
     8521 agaataaaat aaaaacttac taaattcaaa aatagtaaga aagagaggag ggatttagtg
     8581 gtagaaaact taaacagttg acctatcact aaaaaatata atgcttgatt ttatttcaaa
     8641 taatatttta cgtaatccat ctatgttaat tggattaata gcagcatttg gtcttgtatt
     8701 acagaagaag aaagcatctg atgttataaa ggggtcacta cttgcagctt tcggaattat
     8761 aattatggag actggaacag gtatgttagt tagttcaata gcaccaatta atggagcttt
     8821 ccaaagtata agtggtggtg cagtaacaaa aggtttaagt gatgttactt ttactgcaag
     8881 ttatggtgga attataggtt tagcaatgtt tgcgggttta gctattcacc taatgatagc
     8941 aagatttact cctattaaga caatcttctt aacaggtcac atgctttatt ggttcccatt
     9001 catatttgta gcttcaggag ttgaagctgg attaaatggt ggagtattaa tagcttttgc
     9061 agcagtatta tcagcactct attggtcaat aatgccttgg ttattaagaa aatatgtgtt
     9121 tgatgttata ggagatgatt cttttacatt aggtcatcca tctggttgtt tagcacttat
     9181 atcaggattt gttgctaaat tagtaggaaa taaagaaaaa tctacagaag atataaacat
     9241 acctcaaagt ttatcatttt ttagagaaat atctataagt ggtggaatgg cagtgttttt
     9301 aatgtttata gttgttggga tagtaatacc aggtgcgttt aaagatagta atgagtttat
     9361 tggaacgtat gcaataaagc aaggtctttc ttttggagca ggtcttataa taatgcttca
     9421 tggggtaaga atgctaataa atcaaataat gccagcattt caaggtatat ctgaaaaagt
     9481 tgtgccaaat gcactacctg cttttgactg tccaatatta tttaattata aaccaaatgc
     9541 tgtattaata ggattcttag ttgctatgct aacatctaca gctatgattt taatatgtaa
     9601 ctactttaat gtatttaatt tgctacttat accacttgtt ataacttcat tctttgagtg
     9661 tggagcagca gcagtaatag gagaatcaca aggtggtata cgtggagcta taataggtac
     9721 agctatagct gcaatattta tggtagtttt agtaggaata tctgctgtaa tttactcagg
     9781 aactattcaa aattggatac taatatttgg aggtaatgac ctttcattat ttggtataat
     9841 cagtaggttt ataggttcac tatttggagg aatattatag atatgaaatt taaatacata
     9901 gatgagataa gaagattaat tgatataata gaaaacgaag aaaaagtcaa tatggaaaaa
     9961 gcagtacaaa tctttgttga agcaatagaa agtaaaaatg ctatattttc atttggagct
    10021 tcacatgcag gaatattaac agaagaatta ttctatagag caggtggctg tgttcttata
    10081 aatccagtat ttgcaaaatc tctaatgtta gatacagcgc ctataactca cacaagtaag
    10141 atggaaagat tagaaggata tggtacagct ctagctgata aaactccaat taaaaaagga
    10201 gatgtagtta tagttcattc agtatctggt agaaatccag taagtataga gtttgctatg
    10261 aaagctaagg aaaataaggc aatagtaata tgtataacta atttaagtta ctcaaaaagt
    10321 gtatcttcaa gacattcttc aggaaaaaaa ctttatgaag tatgcgattt agtgatagat
    10381 aatcatggtc aaaagggaga tgcctgtgta gagatagatg gattaaacca aaaagttagt
    10441 ccaacttcta ctgtaatagc aactactgtg atgaactcta ttatagctca agctactcag
    10501 gaattagtta ataaagggct aaagaatcct ccgattttct atagtgcaaa tattgatggt
    10561 ggagatgagt taaataagaa gatttttgat gaatataaaa gtgtaattca ttatgaatat
    10621 taaggttaaa aaaatatgtt aatgaacttt gaagtttatc aacaaagaat attaataata
    10681 tataataaat gctatcattt aaaagctgta ggtaaaaatc tacagctttt tattattgta
    10741 gattaaaaag atgcgtaatt aaattttaaa ttggagaaaa taaattggaa ggtatcaatt
    10801 ataaatatta gaaacgttgt catagaaaaa aactatatta aaaaaaagta catagaaaaa
    10861 actgattaga caataaatac atgctttgat aatattttaa atggagtgaa aatgtgtaga
    10921 attataatga aaaaagaaaa taaaaatgaa aaaagaaaat aaaatggagg gaataaaagt
    10981 gggtatgtta aaaaaattat gttcactagc tatggtagca attatgacta caacacttat
    11041 aacagggtgt tcaagtggaa atggaaaaga ttcaaaaaag gatggcgaaa aagaaaaact
    11101 agttctttgg atgccaccag tagaaaataa tatgaaggaa gtttgggacc ctattttaga
    11161 taaatttgag gaaaaaaata actgtgaagt agatttacag ataattcctt gggataatta
    11221 ttctgaaaaa tttgcaacag ctataagtgc tggcgaaggt ccagatgtag gatatatgta
    11281 tgcagaaatg tatccacagt ttatagaaag tggagcagta gaagatttga ctaactatgc
    11341 aactaaagag gataaagagc aatctattta tataaaaaca tctgaaatga tgggtggaat
    11401 gtatggtatg ccattccaag cagcaaatcc aggggtatta tactataaca aggatatttt
    11461 agagaaacta ggagaaaaac ctcctaaaac atgggaagat tttaaaagaa tttgtaaaaa
    11521 agcaacgcaa gatactgatg gtgatggaaa gatagaccaa tggggattag ctcagggatg
    11581 gggagctaaa acttttggta acttaaactg gaactggtat ccttatttat ggcaagctgg
    11641 tggagatata tttaatgatg atttaaaatc ggttaaattt aatgataaga gtggtttaga
    11701 agcagctaag ttcttaaaag aattacaagc atatgtgcct gaagattctt tatctaaaga
    11761 tagtaatgaa atgatagaaa gtgtatttgg accagggaaa gcagcattta caataatgtt
    11821 atcttctgct gcaacaagtg tatttgatga atcattcccg gatttaaatt ggggatttgt
    11881 aactggacta gaagataaga aagcagcaac ttttggagca gtagaccatt tatctttaat
    11941 gtcatctgcg aaaaataaag aacttgcata caagttaata caacatatgt taagtgttga
    12001 atcaatgaca gaatttcata aagcaatacc aagagcacca ataacaaaag gtgaaccata
    12061 tcaaggtgat gaaagattca aagaaatggt ggaaaatgac aaaaatgtat atagaccatt
    12121 agtagtagga cctcatggag ttgaaatata tgaatattta tggaaagaac ttcaaactat
    12181 gatttcagga gataaaactc caaaacaagc gttagatgat gctgcaaaat attcaaatga
    12241 tttattagca caataaaagt ttaaagctgt aatatgttgt aaaggacttt acagattaaa
    12301 attagcattg tatttataaa tacaatgcta atatttatat tggagaggat aaaatggaaa
    12361 acttagttag agagtttgaa gaaaattcaa aaagaaaaaa aacaaaatta aatattaaac
    12421 catatatgta tatattgcca ttgggaataa ttttagtggc gttttatgtg ataccaataa
    12481 taatgtcaat atactttagt tttacaaagt ataatattat tagtccagct acttttatag
    12541 gtttagaaaa ttataaaaaa ttatttacag atgaaacatt aaaagtatct attataaata
    12601 ctattaaatt tactgttgtt gtagtacctt gccaaactat attatcattg attttggctg
    12661 tttggattac aggaaaagga aatagtaaaa tagcttcatt tgctaaaggt gctatattta
    12721 taccagtatt atcatctatg gttttgatag gtatggtatg gagggcttta ttgaatggag
    12781 aaggttctat tatatatcag gtattaggtg cctttggaat agaatcatct aaacttttag
    12841 gagatagtaa tacagcatta cctacactta tgtttatatc tatgtggaaa aatatagggt
    12901 atttcatggt aatttatatt tcagctataa tgaatttacc aaaacactgc tatgaagtag
    12961 cgaaggttga tggggctaca aaacttcaag aatttataaa aataactgta cctttattaa
    13021 aaccaactac aataatggtt gtatttttag ggtcgatatg gtcattacaa gtatttgact
    13081 taatttatac tgtgacaggt ggaggtccag gtatatctac tatgagtata gttatgcatg
    13141 catttaactt aaactttaaa aactttaatt ctggatatgc aatgactgta gcaaacgtat
    13201 tatttttgtt aatagcagta gtttcgattt tacaaaataa gcttgtaaaa cgagacaatt
    13261 cagattttta gaaaggtagg tataatatga aaacagcaag aagattatta agtgatggtt
    13321 tacttttatt tatagcatgt gcaagtttgg taccttttat atacatgttg ataatatcat
    13381 tgaagataac atataactca tatagtttgg atatatcttt ttcaacagta acccttcaaa
    13441 actatataga tatttttact aaaaaaggat ttgcacaata tttttttaat acagcaatag
    13501 tatctttttc aggtgtatta ttaaatttag tatttagtac attagcagga tattcttttg
    13561 cgaagatgga ttttaaaggt agtgataagt tatttttatt tatgataatg actttaatta
    13621 taccttccca agttacaatg ataccactat atataataat gaaacactta ggatggataa
    13681 atagttatct tgcacttatt atgccaatac caactgcatt tggcgtattt ataatgagac
    13741 aagctatttt aggagtacca aaagagttat tagaatcagc aaagatagat ggttgctcgg
    13801 attttagaat tttaatacaa atagtgttgc ctttaattaa accagcatta attacactag
    13861 caatatttac ttttatggga gcatggaacg agtttatgtg gcctcttata gctacaacta
    13921 aagatgcgat gagaacatta acagttggat tatctactct taagacattc cagataacta
    13981 attatggaca gatgatggca ggggctacaa taacattttt accgccattt attttctatt
    14041 tgattttaca gagtaaattt gttgaaggtg tgtctttgtc tggtataaaa ggttaaatag
    14101 tctaatttaa taatatataa agaaacttat ataaatatgg aggaatttaa actatggaaa
    14161 tttcatatgt actgaaagat tgcacattca aacacaataa atattcagtt atagaagctg
    14221 aaacttggaa agataaggat ttggatattg tagtagctaa aggtgaaaaa tttgcatttc
    14281 aaattatgtt aaaagcaaca gaagaattta attgtacaat tgataaaagt agtgctatat
    14341 catggaaagg cctgggaaat agagtaaggt tagcattaaa tatgcctaat agtttagaaa
    14401 ataattttag tataaatata ttaggatatg ttcaagatga tacaaaggca tttgtgaatg
    14461 atgctatttt aagggataaa gatgtgttag tagagcaata tttgcctcaa acattttgga
    14521 ttgaaggtca agtaccagaa gattttgaag aaaataacct tgatataggt attgatatat
    14581 ttaaaagctt tggatatgaa gatgaagaaa aagtttgtac aattgatgtt cctgtaaaag
    14641 taagaaatgt tgtgctaaag cctttagatg aaagcaagtt ctttttagat ttatggcaac
    14701 atccaagttg tttagctaga atgtataaag ttgagctatg gtctgatata cattttgaaa
    14761 tagtcgataa ctatttaaaa gaattagcat ctttaggaga aaaagttgca acagttatag
    14821 tttcagatta tccatgggca gggcaaagtt gttataaagt ttataaaaat ccatcaaact
    14881 tatatgaata taacatggta agtgtatcaa aagaattgga tggaaagatt aaatgtaact
    14941 ttgagagtat ggataggtat ataagcatag ctgataagta taaaatggct aaggagatag
    15001 atttatttgg tctattaggt aattggtgtg ctggtgagtt tggaaatcct gtagaaggtt
    15061 ataaggaccc tataagagta agatattttg atgaaaagga taaagtattt aaatttataa
    15121 acaatacaaa tgatttaaaa gaatatatag gattagtatt aaatcattta atagagtgtg
    15181 gattatggga cagagtaaga attatagctg atgaaccaaa taatccagaa gtagtcaaag
    15241 agtgtataga atttataaat tctactgtaa gtactcatca agttaaatat aaatcagcaa
    15301 ctcacgacca aaacttttta gacagagcta aagatgaaat tgatgatatg tcaataaatt
    15361 taaaacttac tatacaaaat tataaagata tagaaagtct taagaaaaaa atcaatgaca
    15421 agggtgggat attgacttgg tttgtatgct gttttccaga aaaaccaaat agttttttaa
    15481 gttctccttt tgtagaaaat agaataatag gatggtatac ttattacttt ggtcttgatg
    15541 gatttttaag atgggactat aatttatgga cagaagaccc ttggaaagat tcaagttaca
    15601 aattccctat ttggaaagct ggagatatgt tctttgttta tcctggaaaa gatttaaagc
    15661 cagttagaag tgtcagaatg gagaatttaa gatttggaat acaggatttt gagttattta
    15721 caatgttaga aaaagaaaag ggaagagagt atatagaagt tgagcttatg caagaattat
    15781 taaataaaaa agaaaatgct gaaattaaag gttttggtga catagaacta ggatattcac
    15841 ttgataattg gagatatgat aaagttaaaa aatatgtatt agagttgtta gacagagtat
    15901 aaaaaatata agagtgtctc aaaatgaact ttttagttca tgaggcactc tttttgtatg
    15961 aaatcaatat agtttcattg tttcaacaat gaaaaatagg attatttcta ttctaagaca
    16021 gccctttagt tttttagttg tattcaatgt tttaaaacta actataaacg ctataaagtt
    16081 tataaatgac aataattata tataacatga tgtactttaa tttgctataa tagggattga
    16141 ataatattat atggaagata acattgtttg gggggattaa tataaaaaga ataggtatta
    16201 ttataatgtt ttttttaagt atatctttta ttggatttta tcttgtaata agagataaca
    16261 gtgatacagt taagatgcaa cgagggataa atataggtaa tgcactagaa tcacctaagg
    16321 attttccgtg ggatgtaaaa atgtcaaata agttttttga tgatataaaa gatgcaggtt
    16381 ttgacacagt acgtatacca gtaaggtttt ctgattatac gtcagattct gataatttta
    16441 aaattgatga agaatttttt aaaaagattg ataaatatgt agattatgca ttggataaag
    16501 acttaatagt tgtgttggat ttacaccatt ttgaagaaat tatgaaagaa ccaagagttc
    16561 ataaagaaaa gtttttaaaa atatggcaac agatagctaa taggtatcaa aaatatgata
    16621 aaaaattggt atttgaatta ttaaatgaac ctaaagaaaa tttatattca cagttactaa
    16681 acgaatacat tgaagaagca ataaagataa ttagaaaaac taaccctaaa agaactatta
    16741 tagtaggacc atataatttc tatcaaattg attatttgaa tgaattaaat attcctaaag
    16801 attctaatat agtagtttcc tttcactatt acgagccaaa tgattttgca tttcaaggaa
    16861 atatctatca taagggattt gagcacttaa gcaatattac ttgggaagga acaaatgaac
    16921 aaatggatta tttaaaaaaa aggtttgata ctgtggaaaa ttgggcaaat aaaaataagg
    16981 taaaaatctt cttgggtgaa tttggagtaa caaaagaagc accagaaact tcaaggagag
    17041 cttgggtaaa agctgtaaga gaagaagctg aaaaacgaaa cttttcatgg gcttattggg
    17101 agcttgcttc tggatttggt atatacaatc aaattgaagg gacttgggat agagaaatat
    17161 taagtgcatt aatagaaaag aggtgatttt atgaagaaaa attcaaaaaa gatacttata
    17221 actttaacta ttattactaa tatagtatat atattgtgga gaatttttta tacagtacct
    17281 aaagaagaag ggatgtttgc cctaatctgt gccataatac ttctttttgt agagataatg
    17341 ggcatgatgg aaatgtttgt gcattattat ggtatgtcaa atattgagta ccctgaaaaa
    17401 ccaatcataa gtgaagaact ttatccacat gtagatgtat tcattgcaac atataatgaa
    17461 tctgttgatt tggtgagaaa aactgtaaat gggtgtattc atatgcagta tccagataag
    17521 aaaaaggtac atatatacat atgtgatgat ggaaatcgtg aagaaatgtg tattctagca
    17581 gaaaaaatgg gtgtaaatta cataactaga actgaaagag aaggagcaaa agctggaaat
    17641 ttaaataatg caatgcaaca tacaaattcc cctttgattg ctacatttga tgcagatatg
    17701 ataccaatgc atgacttttt aattgctaca gtaccatact ttttgaaaaa tgagcaagct
    17761 aaaaaagatg gagaaaaaga ggagtatgaa aaggttggtt ttgtgcaaac tccacagagt
    17821 ttttataatc cagacttgtt tcaatttaat ctgcactctg agcgaaggat tccaaatgag
    17881 caagattatt tttacagaga cattcaattg gctagaaata ggacaaattc tgtaatttat
    17941 ggtggtagta atacagttat ttcaagggaa gcacttgaag aagttgatgg attttatact
    18001 tattctatta cagaagattt tgcaacaggt atcttaattc aaagtaaggg gtatagatgt
    18061 tatgctattc cagaagtaca tgcatcaggt ttatctccga cagatttaaa aagtcttatt
    18121 aagcaacgtg aaagatgggc acgtggatgt atacaaactg gtagacgtct aaatattttg
    18181 tttaggagag gtttaggttt ttggcagaaa ataagttata tttcttcaat tacttattgg
    18241 tatgcaagta taaaacgttt tgtatatata atggcaccta ttttattttc tgtgtttaat
    18301 gtgattgtag taaaatgtac attacttcaa gtattggtat tttggttgcc aatgtatgtt
    18361 ttaagtagtt tatctcttaa aatattttct caaaatataa ggaatgttag atggaccaat
    18421 atatatgaaa ctataatgtt tcagtcctta atgccagcag taatacttga aacgtttgca
    18481 atatctaaaa ataaattttc agtaacaaat aagagtaaat tagaagaaaa tagaatgtat
    18541 aagtttttac agggaatacc ttattttatt tacatggtct tatctatcat aggaatttta
    18601 aaaatgtttg tagcaatatt taaaatgagt tccataacat attcagttgt attattttgg
    18661 ttaattggaa acctttttaa ccttgttatg gctacattat ttatatcagg gagacagcag
    18721 ctaagaaagt ctgagcgtta tattgctgaa attgatttta agttaaagca aaattcttat
    18781 gttctttctt caaaaacaat tgatatatca gaaaatgggt ttgcgttttt gctagaaaat
    18841 ccagaatata tttccccaga agaagagttt gaggtagagt ttagagaaaa atctggaaat
    18901 gaaatgtata tagctaatat gaaagcaaag attgttaatg ttgtggaagt aaattcaaag
    18961 tggaaatatg cagcttatat tacacatatt gaagatagtg aaatagataa ttggatgtgt
    19021 attgttcacg atagaatacc tactttacca atgacaatat ctaatcaact aggtttcttt
    19081 gatgatttgc aaattaatgt aaaaaaacgt attgaaaaaa ctagaacatt atcacgaaga
    19141 agtccaagaa tcaatatgaa ttttcatatg tatataaaaa atataggtaa attaagaata
    19201 gtaaatttta attatcaata tgtattatta aattttgaga ataaaaatat ttatcctaaa
    19261 gaaatagcat tggaaattag tgaaggtatt gtactagaat gcgatttatg tgagggtaaa
    19321 attgatgaaa gaggtatctt atatagagta aataacattg acagcataat gcaaaattta
    19381 tttttaagag atgaaatgat ggattggata ttacaaaata agaaaattct tgattcaaaa
    19441 cctaatgaaa agaaagaaaa aagtatagat gagtttgaac ccatggaata tatttaggag
    19501 agatgaaatg aagactttaa aagtgttttt ttgttctgca aaatatttta ttttagtgtt
    19561 tatttttttt ataaatacta cttttataag ttcagcaaca agtgataaaa ttttagattt
    19621 gtcctttaaa aagatagaga ctgacttaag ttctaagatt acatatgaag atacaggtgt
    19681 aaaaatagaa acgaacagtt ctaaaagtga taagaaacgt tatttatata tttaccaaaa
    19741 ta
//
LOCUS       DAFKMX010000061        19650 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFKMX010000061 DAFKMX010000000
VERSION     DAFKMX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.061"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            125..382
                     /locus_tag="LZB08_001753"
     CDS             125..382
                     /locus_tag="LZB08_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315258.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            525..1151
                     /locus_tag="LZB08_001754"
     CDS             525..1151
                     /locus_tag="LZB08_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBM7315259.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(1398..2177)
                     /locus_tag="LZB08_001755"
     CDS             complement(1398..2177)
                     /locus_tag="LZB08_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBM7315260.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(2245..2706)
                     /locus_tag="LZB08_001756"
     CDS             complement(2245..2706)
                     /locus_tag="LZB08_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7315261.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(2682..3359)
                     /locus_tag="LZB08_001757"
     CDS             complement(2682..3359)
                     /locus_tag="LZB08_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7315262.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            3592..3978
                     /locus_tag="LZB08_001758"
     CDS             3592..3978
                     /locus_tag="LZB08_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7315263.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            3981..5780
                     /locus_tag="LZB08_001759"
     CDS             3981..5780
                     /locus_tag="LZB08_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBM7315264.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLVSWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(6135..8009)
                     /locus_tag="LZB08_001760"
     CDS             complement(6135..8009)
                     /locus_tag="LZB08_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7315265.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(8014..9678)
                     /locus_tag="LZB08_001761"
     CDS             complement(8014..9678)
                     /locus_tag="LZB08_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBM7315266.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKAR"
     gene            complement(9692..10513)
                     /locus_tag="LZB08_001762"
     CDS             complement(9692..10513)
                     /locus_tag="LZB08_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBM7315267.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNKRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(10612..11376)
                     /locus_tag="LZB08_001763"
     CDS             complement(10612..11376)
                     /locus_tag="LZB08_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7315268.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(11373..12122)
                     /locus_tag="LZB08_001764"
     CDS             complement(11373..12122)
                     /locus_tag="LZB08_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7315269.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(12626..13231)
                     /locus_tag="LZB08_001765"
     CDS             complement(12626..13231)
                     /locus_tag="LZB08_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7315270.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            13410..14048
                     /locus_tag="LZB08_001766"
     CDS             13410..14048
                     /locus_tag="LZB08_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7315271.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            14180..14842
                     /locus_tag="LZB08_001767"
     CDS             14180..14842
                     /locus_tag="LZB08_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315272.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLRKKLKNPPCIKTLRGIGYRME
                     DN"
     gene            14842..16023
                     /locus_tag="LZB08_001768"
     CDS             14842..16023
                     /locus_tag="LZB08_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7315273.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLTVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(16204..18864)
                     /locus_tag="LZB08_001769"
     CDS             complement(16204..18864)
                     /locus_tag="LZB08_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315274.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(18861..19544)
                     /locus_tag="LZB08_001770"
     CDS             complement(18861..19544)
                     /locus_tag="LZB08_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315275.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         6964 a         3138 c         2308 g         7240 t
ORIGIN      
        1 actaatataa tactcttaaa aactttacaa atcaatttaa atttgctacc ttttatttat
       61 aacaacttat agtacatttc tcctatgctt ttcaatatat tacttttaaa aggaggtttt
      121 cattatgatt ttaaatagag ctgagctttt ccgaggatat tctttaaata gatttaacga
      181 gatatgcaat atattatcag taaataaaat taaatataag tataaagtga aaaataatgc
      241 ttactctaaa aaaccttttt ttaatgaaat aacacttggc aatttaggtc aaaaggaaga
      301 tttctcttat gagtacttaa tttatgttca taaagacgat tatgaacagg cagagtctct
      361 aataaattct aagttgcgat agattattta taaaacatga cattctgttg tcatgtttta
      421 tatgttatta tactttcaaa tatgctacta tattttcaaa attgtgtgct ttaaaactag
      481 ctttttatag taatttaaag cttaaaacta aggagactcg ttatatgaaa ataaatataa
      541 aatcaaagtt aagtgaattt attaaacaga acaatctttt tgatgacagc agaataatat
      601 cctaccttcc taatataaca atagaaacag acaaaataaa aactataatg attaatgagg
      661 ttgtcccaac taacaccaat gacgattttt atagtgtaga taaagatgct gattatttaa
      721 aaactactat tcctttattt gacagtgcag gaataaaagt atctaacata aatgatatcc
      781 tagatatggg tatctatata actaatgcag taaaacttcc taaatcagaa tatacaataa
      841 ctcgtgatac aattaaatta catatgccta ttttagaaga agaaattaaa ttatttaaaa
      901 atctagaggt agtaatgtta atgggagatg ttgcaaaaaa atcttttaat atgattacta
      961 aaaagcatat aaaaaagaat gtcatccctt ccatttcaac atataaactt cgtaataatg
     1021 aactttatta tgataattta agagtatttc catcatatat tatgactggt ggaaacatac
     1081 tgattgaaaa atcaaaattt gaaatggcat cagaagatat aaaaaaaatg ttcgagatta
     1141 taaacggttg atattacaaa ataataaaaa ctaatataaa ataaaaagtg cctcaaaaac
     1201 ttctatttat gaggcacttt tattttatat tagtgtaact atatactatt aacataattt
     1261 attatttata ttgttctttt taatctctat tccatttagt ctaggtaaca ttactcttta
     1321 caagattact ttatatttct tttatatctc taacgttttt atttaataaa acaattatta
     1381 atacaagtca aatatatcta tttatctaaa aatatatctt ttacagtaat atctggatgg
     1441 tgatacttct tcctattcgt taaaaaagat ttttttacat taatagcctc ttttggacac
     1501 cagtgaaaac aagctaggca tctttcacac tcattacccc actttggctt tttatcaact
     1561 atacttatat tctctacagg acaaaccctt ttacatgtcc cacatccatt acatttattt
     1621 tctgcaaaaa atttagtagt tgcaatttgc atattcttac ctactaaacg aagtacaaca
     1681 ctatctatat gtgactttaa attgtttttt ccttctatat ttccaatctc caagttattg
     1741 atacattttg caatctcagc taccctatta gtctgatttt tcaatctttc ttcttcaaca
     1801 tctggaggtg ttatcaaagc atttccaggc atattgatta aaaaaccagc agataatttt
     1861 ttcccccttt ttttaagtaa cttatctata gtaaataaac tatgtccagc taccccatta
     1921 caagtggcaa tggcatatat atgagtatta atattaaaat ttaatttctc tacaaaagac
     1981 tttactatat caggaacatc agcaaaataa acaggaaaaa caattaccaa tatatcttca
     2041 tttatgtcta tattattttc ttttctatgt atagctaagg gtataacttc tttacttcca
     2101 tttaacgttt cttttaaaat atttgaaact actagcgaat ttcctgttcc agaaaaataa
     2161 taaatcttac tattcattct tatccctctt tctatatttc tacatcatca atagttaaaa
     2221 agtctcttaa aataggattt atatttactt tccaattcta tccaaaatat cagtaaacaa
     2281 aggcaattct tcatcagaga tagatttaaa tgtctctgtt gcatatatac ttgcaatttc
     2341 atttaatggt tcttctaatt ctctacctaa atctgttagg aatatttgaa acttacgtct
     2401 atctttaaca tctactttac ggacaattaa tcccttattt ttcataacat ctaaaattct
     2461 tgttgtatta gatttatcct tacatgattt ttcacataaa tctatctgag aaattccatc
     2521 ttgctcccat agaaacctta ataacgcata atggtcagtt gttatatcat atccattttg
     2581 tttaaacatc ttttttaaat catcttttac ttttaacgaa gtacttacta acttagttgt
     2641 agcttcatat agtttctcaa aatctttcat tttacctcca tttaaataat cccttgctcc
     2701 tcccatctat caattattcc cttatattgc tcaatatctt taaaagccat tggctttaaa
     2761 acagcattta tagcaataaa attatagatt tgagaaagaa caggtggatt tttcttttcc
     2821 tctatgcaat aaataaattt tttagatacc ttttgtatct ttgattctac tttttcagtt
     2881 ataggatttt gattaaatcc agtttttata gataaactac cacaaacata acctcccgaa
     2941 tctaagacca tggattttat aaaagaaatt gcttttgaac cacgtgcatt ttcatatgta
     3001 gtaactgcta tacatggctt acgatacaat aatctttcaa gcatcatatg tgctctatct
     3061 gtaaacacct tgaaaagtcc agttacacta cttccataag ttgggctacc aaatataaca
     3121 ccatcactct tagtaattat atcatgaata tactctactt tatcattttg attgatacac
     3181 ttgcccattt tataacaatt taaacaacca atacaatatg atggatttac ctcacttaaa
     3241 tcaaaataat taatttttac atctggtatt aatctttcta tatctttata caagtaagtt
     3301 aaaacttttg atgtagctcc attttttcta gggcttccat ttataactgt aattatcatt
     3361 tctatatctc ctttttagtt gttatatcaa caataagtat atcaactata tttttagctg
     3421 tcaatataaa ccatttattt aaagccatac ttacacagct taaataaaag ttttatttaa
     3481 ctacatgtgc ttatttaatc aactataaat gaattatttt taataattga caaactaaat
     3541 taatgataat atactttatg tattacaaat gtaagtagaa ggaggtatag tatgaaaaag
     3601 ttaccacata tttcagaagc agaatatcaa gttatgaaaa ttatttggaa atatgctcct
     3661 atcagcacta ctgaagtaat tgaaaagttg gtagaaacaa gtacatggag tcctaaaaca
     3721 attcaaacaa tgttattgag attagttaaa aaaggagctt taacatatga aaaaaatagc
     3781 agagtattcg tttatactcc acttgttaaa gaagaagaat acgtagctag agaaagtagt
     3841 tcttttttaa atcgttttta taatggtact cttaattcta tggtactaaa ctttttagaa
     3901 aatgataaac tatctgaaga tgatattgag gaactgagag aaatcttaaa taaaaggacg
     3961 actaaggggg ataaataaat atgtatgccc cattctttat acgattttta ctcagtacat
     4021 taaccttatc aatattaatt gttgtaattt tacttgccaa aaaagtattt aaaaaacata
     4081 tttctgcaaa gtaccaatac aatattggat ttatattgct tataatgtta ataattccat
     4141 ttgtccctct taaatatatt aatcttggaa atgtatttaa ctatttatat acttttaatc
     4201 aacttggaag cagtactaat aggacttcta tgataaaaaa tagtccaaat tctataatta
     4261 ataactcaaa tgcattaaac gatttttcag tatccctaaa ccaagttaat tttgatagtt
     4321 taaatatttt attactagta tcatggataa ttggaatttt aatcatgata gctattacta
     4381 ttcattgcaa cattaaaata aaaaatatga aaaaatcaat acaaatttta agagatgatg
     4441 agatagtatt tatatttgaa aagtgtaaaa aagatattgg tataaataag gattttatac
     4501 tatgcaaatc acctattata aatacaccta ttacatttgg atactttaaa ccacacatta
     4561 tattaccaga taagtctata tctaaattat ctttaaaaga tataaaatat atcttacttc
     4621 atgaattaca acattttaaa aataaagata tactgataaa ttatataatg tgctttcttc
     4681 aaatattata ttggtttaat cctcttgtat ggtatgcatt taaagaaatg cgtatagacc
     4741 gtgaaattgc atgtgatatt tcagttttaa aaaagcttga taaagatagt catatagaat
     4801 atggacaaac tctaataaac tttgtggata atatttcaag accatcaaat cttacactta
     4861 catcagatat tggtgggtca aaaaaacaaa taaaaaagcg actattgaag ataatggatt
     4921 ttcataatga atcaaatacg ttaaaattca aaagcatttt aatttttaca ttgatagctt
     4981 taattgtatt tggaaatatt cccaaactat ctattatggc agatatcaac gaaaaatata
     5041 gacctacaaa tgaacaaact gtgtacgagg acttaagtca gtattttaaa ggttttgatg
     5101 gaagttttgt tatgtatgat ttaaaatcca agcaatatca tatttacaat aaagaaaaaa
     5161 gtaaaacaag aatttctcca aattctactt ataagattta tagtgcctta ttgggattgg
     5221 aaaatgggat aataacaaga aataattcac atatcccttg ggatggaaag catcattcta
     5281 atagttcttg gaatgaagac caagacttat tttctgctat ggaaaactcc gtaaactggt
     5341 actttcagac tctagataaa aaaacagggc taaaaaacat acaaacttat cttaagcaaa
     5401 ttggatatgg caattatgat gtttcaggtg gtatctcaaa cttttggcta gaatcttcat
     5461 taaaaatatc acctattgaa caggtagaat tacttgaaag tttttataca aataagttta
     5521 aatttgaaga taaaaatata gaaacagtaa aagattcatt gttaatatca aaaaaggatg
     5581 gggtatcact ttttggtaaa acaggcactg ctaatataaa tggaaaagat gtaaatggtt
     5641 ggtttatagg ttatgtggaa aaaaatgaaa atacttattt ctttgctaca aatattcaaa
     5701 gcaaagatta ttctaatggt agttcagcat ctaagataac attatccata ttaaatgaca
     5761 aaaaaatcta taatccttaa tatatttact gtacattcta aaaaattcac ataattttca
     5821 tattaattat cgattaaatt catatacaag gcttattata taaacataga agatagtaaa
     5881 actagcttaa aaacataata taatacccta tacaaatagt ggcaactctc cctcacatcc
     5941 tcaattgttg ccactattta attttttgta tgaagttaaa tatattttca tcatttcaac
     6001 aatttgggtg caatacaatc tctattttga aacaacctct ttaattgttt tagagtttat
     6061 gaattatttc tcatacttta aaaagattga attactcttg ataatcttct tttgtagtca
     6121 ttacatatca cctactactc aacacatatt ctttgaccta tttttacatc cttgtttata
     6181 tccaaaatat ttaccttctt accatctgga aacaatacca ttgtcaaagt agactttcct
     6241 aattcgtgta ttctctttaa atcaacatta acaagaacat ctccctgttt aaccatatct
     6301 ccttgtttaa cagcaacatc aaagccatct ccatttaatg caactgtatc aattccaaca
     6361 tgaattaata cttctccatt agttccttta atcccaaatg catgtccact tgaaaatact
     6421 gtttctaatt tacctgaaat aggagaaaca atttttccat ctataatgtc aatagcaaaa
     6481 ccatcaccta acattttttt agaaaatgtt tcatctggta cattttctaa tgcaacaatt
     6541 ttccccttag ctggagacac aaagttttca ttattttcat taaacactaa ttcttcgtct
     6601 tcatcttcat ttatagcttc tttaggcact ccaaataaac atgttacaat aaagcagaat
     6661 gcaaccacaa atattgatac taataagtat gtgattaaat tatatccatc atagatatac
     6721 attaaataag aaggtaaaac tgctaatcca tatgaattag attgaatatg taaaattgat
     6781 aatattgctg ctcctaaacc tgaagatgca cacataataa tcaaaggctt taaattatat
     6841 cgaagctgaa tcccaaataa aacaggctca cttataccaa aaagttgaga tagcattgct
     6901 ccaactgcag tttgtttaac ttgattattt ttagattttt tcataaatgc tagacatgtt
     6961 ccaatattcg caaatccata cattgcacaa agagtaataa gggcattaaa tcctgtattt
     7021 gctaatagtg atgtttcaat cattacataa gtatgatgta agccagtaat aacagctaat
     7081 ggatatgtaa atccaataat aaatcctcca attcctaaag gcaaatcaat caataagctg
     7141 atgatatcaa ccatttttaa ttctattgta tgcattatag ggcctacacc taacaaaata
     7201 actaacattg ttattagcat aacaaaaaat ggtgtgaaaa taaggtctaa tacattaggc
     7261 atatgtttac ggaaccattt ttcaagatta gctccaataa atgctgtaac aatagctgtt
     7321 aaaacacttc cttgacaacc tacaatagga ataaatccaa atgccataat tgcttcaatt
     7381 tcatttgatg ggtcagcaac agaatatgca ttaggaagta ttggggaaac taacataagt
     7441 ccaataacaa gtccaataac aggtgttcct ccaaatactt taaatgctga ccatacaata
     7501 attgctggta aaaaagcaaa tgctgtttct gtaagaaccg aaacaagtgt ctgaatatac
     7561 aatggaatat ttgcagaaga cattccaaat aatccaagta cattatcatt aaacaaacaa
     7621 ccctttaatc ccaagaataa tcctgttgct gcaataactg gaataatagg aacaaatata
     7681 tctgctagag ttctcataac tttcttaaat ccagtttcat tatttttaac taattcatct
     7741 tgttcttttt ttgataaagt ttttaatccc attttttcaa cttctgcata aactttatta
     7801 actattcctg ttcctaaaat aatttgatac tgtccagaag taaagaaaac ccctttaacc
     7861 tcatcaactt tttcaacttt cttatcatta ataatttctc tatctttaac catcactctt
     7921 aatcttgtgg cacaatgtgt aatagaaata atattatctt caccaataat tttaataagc
     7981 tcatttgcta ttttattata tttattcatt tttctacctc gctttcaata atactgcatg
     8041 aaatggtttc aaacgcactg ttttatttaa tagatttata tcatctaaat tttggaatac
     8101 aatatcatat ttttcttcta ctcttatttc tacaggttta tcagaaaaat tataataaca
     8161 atcaataact accaaatcta attgccttct atacgcaata acatcttcac tttctatatc
     8221 aataggttct attgaaccat aaatcaaaca atcagaatac tcactatctt gtctcaactt
     8281 tatcatcttt ttataaaatt ctaaaatact ctctgaattg ttaatctgac tctgtgcatt
     8341 aatatcttta taattttcaa taacctttaa ccatggtgtt gtatctgaaa aacctgcatt
     8401 ttctttatct gcccatggaa atggcgttct tgcattatct cggcttctta aattcacaaa
     8461 ttctaacgct tcttcttctg agtaaccttc atctattgaa cgatagtatt ggtcaatgct
     8521 agataaatca ttaaaatcat ttatggtatc tcttttaaaa tttgtcattc ctaattcctg
     8581 tccctgataa ataaatgggg ttcctcttaa aaagaaatac attgctccca acatttttac
     8641 tgcatcatca ttcctttcgt attgcaaaag atactttgtt gtagctcttg gttggtcatg
     8701 attttcaata aaattagctg cccatccatg ttcctgaaca tacatttgac tatctaaaat
     8761 gcattttctt aaatctggaa ttgtccatga aattctttta aaccactcac taccagaagc
     8821 aacatctaag tcagcatgtt taaagtcaaa gaccattgaa aagaaaccat cttctccaat
     8881 aaaatcacct aactggtcat agctaatacc tgctgcttca gcaacagtca tacaatcata
     8941 ttttttaaac gtattttctt ttaattcata aagcattttt tctattccag gctgatttct
     9001 aactgttttt gtacatttaa caagaccatc aacaccatca ctttctaaac tttgaaaact
     9061 taaattcttt ttgataaatg taatagcatc aactctaaat cctgccactc ccatttgaga
     9121 ccaggtatta atcatttggt aaagttcact tcttaatatt ggattttccc aattaaggtc
     9181 aggttgtttt ttaccaaaag aatgaaaata atattcgtct ctattctcta ccttttccca
     9241 aacacttcca ccaaaaacag acctccagtt atttggtaaa tcgtttcctt ttttaaaaat
     9301 ataataatca tgataaggac tttctggatt ttttaatgct tcttgaaacc aaggatgttc
     9361 atcacttgta tgattaagaa ctaaatctaa tataattttg attcctctat ctttactttc
     9421 tttaattaac tcttttaaat cttccatact tccaaattca cttgcaatat ccaaataatc
     9481 actaatatca tacccattat cgtccatagg tgatttaaaa attggacata accacaatga
     9541 tgtaatacct aaatttttta aataatctaa acgagaaata attccctgta aatctccaat
     9601 tccatcatta ttactatctt gaaagcttct tggataaatt tgataaacga tttctttttg
     9661 ccaccatttc ttttccataa attactcctt tttatatgat atttcttctt tttaattctt
     9721 tataaattcc attatcgaat atgtcctcac atatagatgt cgctatatct ttaagttgtt
     9781 gatgactatt tttcattgca attgtaacgt ctgatgcttg aaacatgaca atgtcatttt
     9841 gaccatcacc aaaacatatg gtttcttttt gagttactcc taatctttct tgtaatctct
     9901 taattgcttt gcccttatga aaatcttttt gaataatttc ataatactga ctagaaatat
     9961 ctctctgtgc taattccatt ttatcttgta gtatatcttt cacttcgtca aatacctttt
    10021 catttgacca taaacaaatt ttatgtatat cttgtgactt atactcttca atgttatttt
    10081 catatgttat tttttcttgg atatgttgtt tatttataca agaattagtt cctttcaact
    10141 gattcattgt ttcaaatatt tctttggctt tttgattcat gaaaactttt tcctgactct
    10201 caatcgaaaa agcaacttct ctctttttta ataaacaaac aacttccttt atcaatcttt
    10261 tattaaaaga ttgattataa agtaattcac catgatattg aatgtaatta cctcctcctg
    10321 caatatatcc atcaactcct aatgataaaa catcatcttg tattgtaccc atacttcttc
    10381 cagtacaaat aacaacactg caatgattct tttgacataa acgtatagca tgctttgcag
    10441 attctgggat tccatatacc tcatctctaa gcgtaccatc tatatcaagt attaataatt
    10501 tgtacaattt catcttcctt tcttactatc aaaatcataa tagcattcaa aaaaaaagat
    10561 atcaatattt tgatatcttt taaaaaatat gttcattttt agcacaaatt ttcattttcg
    10621 gggaatatgt tcactttcta aatacatctt gtatttaata taaagcagtt caattaaaat
    10681 aaaataaggt gttgttacat cgtagtccag ttgtttatct acagaaatag atatagattg
    10741 aatatacagg ttttcatcac ataatcttgc taaaggatta cttatcattt ttgtaataga
    10801 aaccatataa attcctttca ttctcatttc tttagcaaaa tcaataattg tagatgcatc
    10861 tccattcaat gaaataaaga aaatcatatc atctgttttt ataaaatgat taagtgcctg
    10921 aatcatatca taaccataaa catcatatat aattttccct gttggtaaga aaatcctttt
    10981 catctcttta gcaactcttt cctgagaata accttctcca aagacgatta tacgatttgc
    11041 ctttttcatt ctttcaaaca taatcgaaca atccttcttc tctatatcat ctatcacctt
    11101 attataacaa tccattaatt ggtgtttact ttgattttta tattctactt ctgtttcatt
    11161 taagcgtata ataactctta attcactata tccaggtaat tgtagttttt gagcaaaacg
    11221 agaaagtgca cttgtagata tatggtattt gtctgcaaat tcatctatag aaagtttaat
    11281 acaatcttct ttatgattaa gaatacactc acagatatac ttatcatttg ctgtaaatga
    11341 attataatat ttattgaaaa gtccttcaat tttcattctt ttctccttct atactctatg
    11401 taattcatat gaagcaaatc taatagagca taaaatgctc ctacgacttc ataagatata
    11461 gcttgtaatg tatcaagttt ttgcgtttca acatacagat tatactcaca aagtgttgca
    11521 attgtattat tatcaagcct agttaaagaa atcgtatgta tatgattgtt gataaatttt
    11581 gcaatttcaa ttcctgcttt tgtttctcca gacaaagaaa tgataataaa taaatcactt
    11641 ttttgaaatg attctctcat aaattcaatt tcacctatat caaataagtc aataacacat
    11701 ttacctactg aaagaaatat tctctttatt tcttgagcta aacttttctg ttcattacct
    11761 gtcccataaa tataaattgt ttctgcatca tatattattt tacaaatatc ttgaaatgat
    11821 attttttgta attcattcat taatagtcga taattctcgc aaacatcatg aaaattatta
    11881 tcattattaa caaattctag ttgattattt ttaagtaaat actttaaatc agagaatgaa
    11941 tctaatccaa tttttctaca aagccttaat aaagtagctc tagaaacatt acattcattt
    12001 gcaatttttt cacacgaaaa attacggtat tctctttgac aaatcaatat ttgtttaata
    12061 ataattttat cattttcatt taaattatca taattttggt taactaacga ttctaatctc
    12121 attttttcca ctccctaaat attattttaa tatacattag ttgctttttc tagggaagag
    12181 ttttaaataa taaggataaa ataatcattt gattttattt ttattaaaat aaaaataacc
    12241 tgctaaaagc aggcaccaaa aagtacttat cctttcggac atgtacttta gatattgctt
    12301 tcttcagaag cttcatctct tcatactcta aaatagagtt ttttctaact caaaagtatt
    12361 ttccatggta gaaattactt taattcatct gccatttatt ttttatctta tttaataatt
    12421 actattactt gaatatttac ttctttaagt aaatttcata gttaacaaag ctaaagctat
    12481 tattatagat gttaaaatac aaaattgtaa gtattgttat aaatatgctt acagctacaa
    12541 atatacatga tatattaaaa agaagctgta aattattatc atacatacaa tcaactacgc
    12601 ctctattaat ccatgttaat ttttattatt taatacaagg agttccatga gacatacata
    12661 tccattcata ttttaaatta tccaattctc ttatagattt catcatcttt ttataatccc
    12721 aattccatgg attaggataa ggaactatat ttccattctt atttttgact aaatcacctg
    12781 caaataatac atcttcaaaa atcatgcaga catgccctgg agtatgacca ggagtatgga
    12841 taatttttat attcccaatc tgcatattct ctccataagg ttcaacatcc ttaatgagcc
    12901 ttctggaaat tgcctttcca ataaacttct taaatccagg tctatccttc tctcctataa
    12961 taaaaggtat atcctctaca tgagcccata cttttgctcc tgttttttct ttcaataatt
    13021 taatattaga tatatggtca acatcatgat gagttaataa aatatgctta atatctgtaa
    13081 gtttaatgcc aagtgttttt aattctttaa ttatattttt ccccataaaa ggaagacctg
    13141 tgtctataag aataatttct ttatcaaaca caataaaaca atttgagcca cgagtactat
    13201 ctaacattaa tacattttct tttatcttca taaataaatt ccccttattt ggtattttac
    13261 ctatataatt aattaataaa gttataaata gttataatac ttgatattaa ttttattttt
    13321 tattaaagta tactataggt atacaagtat tatatttaca taagtatacc atcagtatac
    13381 ttttttatca atagaaagga ataatttata tgtcagaatt atcaaaaaga gataaagaaa
    13441 aaatacaaag agaaaatgag attattgata aggcagaaaa attattttgt cttaatggat
    13501 ttgataatac aactatgaat gaattagcaa aagaagttga atatactaaa cgcaccatat
    13561 acaagtactt ttcatgtaaa gaagatttat tctttgctgt tgtcttaaga ggatataaaa
    13621 gactttggga taatgttaaa atagaaagtg caaaaggcaa aacagggttt gaaaaaataa
    13681 aactttcata ctttgctttt cataaatttt actctgtaga accttctcta ttatgcctta
    13741 tgggaatgat tggtattgta aactctgaaa aatcggatac tgatatgctg tttaaagaaa
    13801 agtttttttc ttttaataaa tttatgtttg atgaaataca aggaatgttt gaaattggaa
    13861 aaaatgatag aagtattagg catgatattg aaatacctac tcttatgtat tcatcaatat
    13921 ttacacttac aggatttttt aatttactat ctgtaacagg aaaatcatac ctgaacaact
    13981 ttaatataga tgaggaaaaa tttattgaaa caactcttgc tcttttaata gattcaatta
    14041 aagcataaac ctaagtttat aaattaaagt ttatatactt aaatgataat tttttctatc
    14101 tctaatataa aaaatattaa acataaaact aataacttag ttaatggaat aactaaatta
    14161 ctgaaaggat taaaagtaaa tggctaaaat acttctttta gaagatgatg atacaattac
    14221 atttggaata aaaacttccc ttgaaaagaa aaatcacact gtatgttgtt ataatacgat
    14281 tgctgaagct aaaaaaaatt ttgataaaac tattgattta attcttcttg atttaaatct
    14341 acctgatggt attggatacg agttttgcca atatgttaaa agtatagaag atactccagt
    14401 aatatttttg acagtccgtg atgatgaaaa agacattata cgtggattag atatgggtgc
    14461 agatgactat gtggttaaac cgtttttgct ctcagtactt caatccagaa ttaatgcagt
    14521 tcttagaaga actcattcaa atactaacaa tactattaca tgtgaaaata taagcttgaa
    14581 taaggttgaa gtcaaagcat atttagataa tgttgaagta cctttgacag caggagaata
    14641 taaacttctt ctaagtctta tggaaaataa aaatagagct atgactcgta caaagttact
    14701 agaaatgctc tgggatgtag atggtaattt cgtaaatgat aacactctta ctgttacaat
    14761 aaaacgtctt agaaaaaaat taaaaaatcc accttgtata aaaacattaa gaggcatagg
    14821 atatagaatg gaggacaact agtgaaaact ccaagaaata tcaacagtac agttgttttt
    14881 ataatattct gcttaattgt aactttttta ttgactgtaa ttctaacaac tataacttct
    14941 tttaatgaat acaatgtact gtctaaggct cttggtgcat catatgagta caatcctaag
    15001 tctacagaaa gtatactaaa atcactgaaa aattctagtg ataaggattt taaaacagga
    15061 aatgaaattt tatcaaaaca tggatattct aaaagtacat tttggagtaa aaactttttt
    15121 tattttctag tgatatcgtt tggtataatt tcaacaatgg ctacagttat atatataatc
    15181 aaacatagaa caaataagca aaaactgagt agaataaaca atcttactca atacttagaa
    15241 gaagtgaatt taggaaaaga gtctgtactt ttacgatgtg aagatgaatt ttctcacctt
    15301 gaagatgaga tatataaaac agttggggaa cttcgttgtt caaaagaatt tgctttaaaa
    15361 gaaagacaaa ctctttcaga taatcttgca gatattgctc atcaacttaa gactccaatt
    15421 acctctatgt cacttatggc acaattactt tcagaaaatt gtccagatga agaaatagat
    15481 tatattaaca gacttacaaa tcaaatctca agacttgaga gactagtttc atctttactt
    15541 acattatcaa aattggatgc aagtacttta atatttgaaa gcaaacttat agatgtacat
    15601 tctcttctta cttatgcaat tgagcctata gaaggttctt taagagaaaa acatcagaca
    15661 tttactatcg aggcttctcc ttcggtagaa tttgaaatcg atattaattg gactttagaa
    15721 gctcttttaa atattcttaa aaattgtagt gagcatatag aagatggtgg atatatagtt
    15781 gcttattata gtcaaaatcc tctatatgtt gagattatca tagaagataa tggtaagggg
    15841 tttgctcagg aagaacttcc atatattttt aatagatttt atcgtggtaa aaatgctagt
    15901 aaggacagta ttggtatagg gcttgctcta tcaaagtcta ttatagaacg tcaaaatggt
    15961 actatacatg cagaaaattg ccataatggt ggtgcaagat tcataattaa attttacaca
    16021 taaaatgctg gattataggt aaaataccta taatccagct atatctatat tctatgttat
    16081 ctattgtttt agtaatatac ttctatttac atttaaggtt ttattaaaca aacaacttaa
    16141 ctatccctat acttgtaagt agtcttaaat cgaaaccttt aaataagttc aaatagattt
    16201 tgtttatatt gtttcatttt ttatagattc tactatattt tcattcttca actgcttgta
    16261 tcctgtcata tatgatgcta ttacaataaa aagtatcaat atagtaaaac ctattattgt
    16321 agatataggt gcaaatggta aatactctat aaaatatatt tcatttatac gtagaaaagc
    16381 atacactatt cctatttgca caggaatact aagtagtaat ggtgtaattc ctaagaataa
    16441 accttccaaa ataagcatct tttttatgcc ttctggagat aatccaacag aacgcaacat
    16501 agcaaactct tgtcgtctac ttctaatatt tcctgacact gtagcaagta catttgataa
    16561 tccaatcata gccaataaac ctgtaataaa gcctactatt aaattcatgg tctttattcc
    16621 actagccttt tgtttttcac tttctaaaac atcctcaata ctataatctc ctgaaccata
    16681 gtctttatta taaatggact ctaatttttc tcttactcta ggaatgtcct cacgattatt
    16741 tactttaaat actcctatca gcctatttga acgtaaccta cttacttcat cataattttt
    16801 agatatttcc tgcaccatag acattggcac tatttgcatt aatgtatatt ttgaactagt
    16861 atctactatt ttaggaagtg tgcttgtaat agctcccact ttcatgttgt aagtataatc
    16921 ccctttatct tcgtcatata ctttttcatt taatttaatg ttatctccta ttgaaagatt
    16981 taaataatct atatatactt tatttctacg tgtactacga ttaatatctt cctccttatt
    17041 atatacaatt gaggttggat tttttgtatc ataaaataac tttgggtcta cagaaatttg
    17101 cctacaatat tctgtaaaac tcttatcatc aagcccaatt atatttgaaa ttatacgaaa
    17161 cttgccatca cgttctatag gagagtattt tttagatgaa actatactct taaatcctcc
    17221 atttttcgca agttcttttg atgcatcttt ttcagtcagc caagtggaag cagacatttt
    17281 ttgataatat agtgaacttt ttacaccttc tgtattatct aatttttcaa tgaaagcaga
    17341 ctctacaggt tcactatttt ccaaataaac tgctatatct ctttggtcct tataattatc
    17401 acttccataa acagcttttg ctccttcttg atttgcaatt atatgcaaaa aacatgtaag
    17461 taataaaaaa gataatgtta aagatattgt tgcagtacga taagattttt tacgagcacg
    17521 tagtgcgttt gatgcaagtt ctccttctat tccaaatatc tttcctaatg aatatttatg
    17581 cgactttttg attttaacac tatctccttg tcgtattgct tctataggag atatctttga
    17641 tacttttcgt gctggtataa gtgcagaaat ccagactgtt actattgtaa gtaaaactgc
    17701 tggcagaaaa gcaggaatac cataagtaaa taccacttct ggaacatcag tatgataatt
    17761 aactgaatta atataaacta taagcctatt acacaataac catcctaaaa ataaacctat
    17821 tggtaatggt ataattgtca gtaaaaagcc ttcaaacact acagaccttt taatttgttt
    17881 tggtgatgca ccaacactag ataatatccc taactgagta agtctagcat ttgcagacag
    17941 tgaaaacgcg ttgtaaatca ccattacaaa cacggctact gtaaaaattg ctattgtaag
    18001 atacataata ggagttgaaa attttgatat actactcata gatgctttct gttctggtga
    18061 aaagactaac atctttaaaa gataattact attgtattta agattgtatt ttccatattc
    18121 attagtcttc catcccaatg atttagcaag ttttggaagt tccttatacg tgtctcttat
    18181 atttttaaat cgcaagtaca cagttatact atcattaggt tttatacttg ttttatcaag
    18241 atacccaaga gctgtatatg caggtattac tgatgaagta gaaacatcta cctttcctac
    18301 tattgtcaat gtagcctctg aaattttttt aaaactttcc cctttcgatt ttggggctat
    18361 gggttcaaga ggctttccat ttaggattct atctcctatt gggagagtaa ttttatcacc
    18421 aatttttaca cttggattat tctcaaaata ttgttttgat aatgcaattt cattagcatt
    18481 atttggtcga cgaccctcta ttatagtatc cttttctggc atagaattcc aatactctga
    18541 tgtagcattt ctataaatta tataatctct tttctcatca ttaattttgc ctacatacca
    18601 ttgtcctttt accattacat catcaactga agaaaaattt tcaattaatg gtaagtcact
    18661 tccataagta tagtcaaaca attcaccatg ccagttacct tctttctcaa ttgaaagttt
    18721 tataaaatct gtccacatgg tatacaacag tccacacaaa gaagacatca tggttgtagt
    18781 aagtaatata gctaacatta tagctatact actacgttta ttgcgtcgta caaagtctaa
    18841 ggtgtattct tgaagaatat tcatttcttc accacctttt cagagacaat cactccatct
    18901 tccatagtta caatacgatt tgcctctagt gctattttct catcatgagt aataagaagt
    18961 attgtctgat taaatcgttt atttgataag tttaacaagt ctacaatttc ttctgaattt
    19021 tttctatcaa gattaccagt tggctcatct gctaataaaa tagctggtct atagattaaa
    19081 cttctagcta tagataccct ttgttgttgt ccacctgaaa gttgacttgg aagatggttt
    19141 aatctatcag ataatcccaa tatagataca atctcagaga atctatcctc atccaccttt
    19201 cttttatcaa gtaacatggg taataatata ttttttctaa catcaagggt aggtataaga
    19261 ttataaaatt gataaataag acctactttt ctacgtctaa aaactgcaag agcttcttcc
    19321 tttaaacttg atatttctgt atcttcaaca taaatctttc cagatgtagg tctatctact
    19381 cccccaagca gatgtaacaa tgtagatttg ccagaaccag atgctcctac aatggataca
    19441 aaatcacctt tttgaactga taaatttaca ttattaaggg ctgtcactct ggtttgattt
    19501 gaaccatata tttttgtgag attttcacat tttaatattt ccatatacaa tcatctcctt
    19561 tatggttaaa ttttagcaac ctaaaatgac attatagtga catgaataca aatataaatt
    19621 actattaaaa atattgttta gtttttgcac
//
LOCUS       DAFKMX010000062        19449 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFKMX010000062 DAFKMX010000000
VERSION     DAFKMX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.062"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(34..633)
                     /locus_tag="LZB08_001771"
     CDS             complement(34..633)
                     /locus_tag="LZB08_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315276.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(834..1046)
                     /locus_tag="LZB08_001772"
     CDS             complement(834..1046)
                     /locus_tag="LZB08_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBM7315277.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(1119..2834)
                     /locus_tag="LZB08_001773"
     CDS             complement(1119..2834)
                     /locus_tag="LZB08_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315278.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2834..3388)
                     /locus_tag="LZB08_001774"
     CDS             complement(2834..3388)
                     /locus_tag="LZB08_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBM7315279.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(3381..3983)
                     /locus_tag="LZB08_001775"
     CDS             complement(3381..3983)
                     /locus_tag="LZB08_001775"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBM7315280.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(3992..6640)
                     /gene="polA"
                     /locus_tag="LZB08_001776"
     CDS             complement(3992..6640)
                     /gene="polA"
                     /locus_tag="LZB08_001776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBM7315281.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            6792..6879
                     /locus_tag="LZB08_001777"
     tRNA            6792..6879
                     /locus_tag="LZB08_001777"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6826..6828,aa:Leu,seq:caa)
     gene            complement(7066..7509)
                     /locus_tag="LZB08_001778"
     CDS             complement(7066..7509)
                     /locus_tag="LZB08_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315282.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(7512..7709)
                     /locus_tag="LZB08_001779"
     CDS             complement(7512..7709)
                     /locus_tag="LZB08_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315283.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(7901..8767)
                     /locus_tag="LZB08_001780"
     CDS             complement(7901..8767)
                     /locus_tag="LZB08_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7315284.1"
                     /translation="MEWINKLNQAITYIEENLKSGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(8993..9862)
                     /locus_tag="LZB08_001781"
     CDS             complement(8993..9862)
                     /locus_tag="LZB08_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7315285.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(10368..10589)
                     /locus_tag="LZB08_001782"
     CDS             complement(10368..10589)
                     /locus_tag="LZB08_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315286.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(11090..12070)
                     /locus_tag="LZB08_001783"
     CDS             complement(11090..12070)
                     /locus_tag="LZB08_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315287.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRNLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(12234..13106)
                     /locus_tag="LZB08_001784"
     CDS             complement(12234..13106)
                     /locus_tag="LZB08_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7315288.1"
                     /translation="MIIKVSMTVLVLILLYILYKYEIQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(13270..13821)
                     /locus_tag="LZB08_001785"
     CDS             complement(13270..13821)
                     /locus_tag="LZB08_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315289.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(13841..14743)
                     /locus_tag="LZB08_001786"
     CDS             complement(13841..14743)
                     /locus_tag="LZB08_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBM7315290.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(14758..17130)
                     /locus_tag="LZB08_001787"
     CDS             complement(14758..17130)
                     /locus_tag="LZB08_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBM7315291.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(17351..18040)
                     /locus_tag="LZB08_001788"
     CDS             complement(17351..18040)
                     /locus_tag="LZB08_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315292.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(18281..>19449)
                     /locus_tag="LZB08_001789"
     CDS             complement(18281..>19449)
                     /locus_tag="LZB08_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7315293.1"
                     /translation="LAFIAWGGYYNYFYKMGYIKYSIFFYLFSVYYKCLDAVIYRILF
                     FAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKSIKLISQDKRNYVY
                     LLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEII
                     KENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEIN
                     EYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEMCLKKGIKFTCSINFSKVNFVKPID
                     VSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCENSKLNQLTFNKNIL
                     LTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT         6174 a         3425 c         1849 g         8001 t
ORIGIN      
        1 catgcaaatt ggcagaactt attctttaaa actctaactt ttaagttttt ctttgataat
       61 ataatttatt actttttttc taatatcttc agcattataa tcatcttcta cttctatatc
      121 caaaagtttt gctattgacc tcatatcttc tctagtcttt gacctatagc ttgatattaa
      181 ttcatctttt attttctctg gttctaaaag atattcttca tctcctcttt tgaaaacaac
      241 gtatttttga gcatatgaac ttctattgta gtatatatat cttgatattt ttcttaaaat
      301 ttgattatat gaaagtcgct tgttaatatc aagttttaag tactctgaaa aagcaaatag
      361 ctcttttttt gatttaaata atgcttcttt ctttatagtc cttgatattg aagttacact
      421 ttcttgatat gcacactttt ttaacatttt taaatatact ctattaagac caatactttg
      481 attgaatttt tttatattat tattaataat cttatccaag ttaccttcaa agtatttttt
      541 tgctcctttt tgtgatacat aactctcaag ttctttgtgg gccatatcaa gtatcatttt
      601 ttttaaatcc tctttatatc ccctagattc catgatactc cctcccttag ctatttatag
      661 tatattcaaa aagctcaaga aatgtgagtc attacaaaat ttttttactt tttatgtata
      721 tattaattta aaaggggaga taatagtaat gtaaaatcaa acatactcaa tctcccccat
      781 ttttagacct ctattactcc cccaatagag gtcttttttt agtttatcaa tttctattca
      841 attcccttta gttcaaatcc taattctgct atcacttctt ttaatttttc aatagataca
      901 tcatctttca tatctacaga tgcacactta ttttctaaat ctacatctaa aacttcaata
      961 ccatctatat cttctgttaa tgcagtttta agatggttta cacaatgacc acaactcata
     1021 ccttctacta atagtttctt cttcatttaa ttacctcctt ataccctata agggtattct
     1081 atcatatagt ttatactttt taaatagaaa ttaatcattt aatttgattc attcgatttt
     1141 atatctaaat tagatattcc ctcatataca tcaaaatcat ttggagtaat tttacctttt
     1201 aatctactat tacttacaat atagttgttt ctcacagcta aacttataaa tgttgcatta
     1261 tcttttatat atgtctgtac atctttgtat gctttttttg tttcaatttc agacgtagaa
     1321 cttgtaagtg cagacatata tccatttaac ttatcatcag tatatccact actcgctata
     1381 atactacttg catctggtat aattgatagt ttccatccaa ctaatgctaa atcataattt
     1441 ttagaattaa gagcattttc catatcctta tctgacagct gatttactgt actatttatt
     1501 cctatagctt ttaagttttc atttattatg tttgcagttt ttattctctc actattatct
     1561 ctgtttacta ttatctttaa gttcatttca gaaagacttt tctttacttc ctctttttct
     1621 ctattttttt tctcttcagc ttcttttttg attctatctt cttctgcttt tttagctttt
     1681 gcttcttctt ctggcgtaag cttctttgag ttctttttag ctgatttttt ctgatttttt
     1741 actttattgt cattttgatt tactgagttt acatttgcat attctacctt agcaagacac
     1801 tctatcgcct tatctttatt aaactctaaa tccttcattt cactattata gtattttgat
     1861 tttgaattaa gtggaaaatt aactggagta gcatcatcca tataaccttc actaataatt
     1921 ctatctttat ctatagagtg tgcaattgct tttctaaaat taacatcttt gaaaaaagga
     1981 ttattataat taaataatac acactcataa tctcttcctt gatattttgt tatattaaac
     2041 tctttttctt gaaacttact taaatcattt aatgtaacat tggttatatc actatcaaga
     2101 gccataacca tagatgtcct tgcatcttca ttaggcacca tacctacttt tatgttcttc
     2161 atggtttttg gaacttcttc atagtaatct ttgtttacag ataaaaccat tccttctctt
     2221 tctgtatatg attctatttt atatttccca tttcctatca tattattatt cttgtcattt
     2281 acatcttttg aatctaaatg attttgagat acaattggaa aaattaaact atcaattgaa
     2341 aaagcatatc tagctttaaa ttttattgta aaatctttat ctgatttaat attcacagaa
     2401 gctatatttt tagtaaattc attatatgga ctatctgcac ttttttgaat taaaccaata
     2461 gtatagttta catcatttga agtaacattt tttccatcat gccactttgc atcttttagc
     2521 tttatatcta tactcattcc atcctgagct atattatact ccttaactaa ttgaggaatt
     2581 acattgtagt tctcatctat agtaaataaa ctatcataaa ctaaattcat tatataacca
     2641 acagacttat cagtattagt tataggattt atagtttttg gagttaccat tgttaagttt
     2701 atatacttag catctataga actttgactt tgtaaacttg aatcactact actttgtttt
     2761 gttttagtat tactacaacc agctaccata agagttatag ctaaaacgac tgttaagact
     2821 tttattctct tcattagttt tttcctttct tgccatatat cttattataa tgttcgtaat
     2881 tattttttac tttttctaca tattttgaag tttgcttaaa aggtatatca tgtagatttt
     2941 cgccatcttt actatattca ttattttcta accatttttt cacattacct gaaccaccat
     3001 tatatgcagc tatcactaaa tctaatttac caaattcttt gtataactta cttaaatacc
     3061 aacagccaag tcttatatta gtctctgggt cgaaaatatc cacatttttt aaatttagtt
     3121 cctctgctcc ccaatctcta gttatgtcta atatttgcat taatcctttt gcttcctttt
     3181 ttgaaacagc agaactatta aacttacttt cggctttaat aacactgtaa actatatttt
     3241 catctaaatt aaattctttc gaatactttt ctacatactc tgaatatttt ttaggatata
     3301 aaaatttatg tattactttg ctttccatta atagtgcccc aaataagatt ataaaaatag
     3361 atagaatcaa tactttctta ctattcacgc caattctcct tcatgtactc aataaattta
     3421 tgtgctttac tttctaattc agttattgtt cccgaattat ctattatata atcaccatat
     3481 ttggattttt cttcctgact catctgtgaa tttatcctac taagtgcttc ctgctcactg
     3541 caattatctc tcttttgtat tctactaatt tgtacttctt gtttacaagt aactactaat
     3601 agcttgtcaa ccatatctaa aaaaccactt tctaccaata ttgccgcatc taacactact
     3661 atattttcgc ctttttttct caatttttct atttcactta ttattttttc tcttattcgt
     3721 ggatgagtca agttatttaa ttccttaagt ttttcttcat cactaaaaac tattttccca
     3781 agagcttttc tatttagagt tccatcatca ttttttatac tttgcccaaa atggacaaat
     3841 actttttcca ataacaactt atcctcaaat atcttcctgg atataatatc agcatccact
     3901 ataggaatgt taagatttct aaatatattt gataggctgc tttttccaca gcctattcct
     3961 cctgtaagtc ctaatatcaa cataaaatca cctactttgt ctcataccaa gaactaccta
     4021 catttaaatc tacatctaag tgaacatcca tatttacagc attttccatt tcttctttta
     4081 ctattttctt aactatatct atttcatcat caattgcttc aactatcaac tcgtcgtgaa
     4141 cttgtagtat aagttttgat ttcaaattgt tctcttccaa ttttttatga acatttatca
     4201 tagcaatttt tattatatct gctgcacttc cttgtattgg tgcattcatt gcaaatctct
     4261 ttcctctatt tctttccata aaattgcttg actttatttc tggtatatat cttcttctgt
     4321 ttaaaattgt tacagcatat ccatcctcac tagccttttc tactgttgtg tccataaact
     4381 cttttatttt atggtattta gcaaaataac tttcgatata ttctttcgcc tttggaacag
     4441 gtatatttaa atcatcagct agaccaaagt cacttttacc atatataata ccaaagttta
     4501 cagctttagc tgcacctctc aattcactag taacatcttc cataggaaca ttaaaaactt
     4561 gtgaagctgt tttggtatga atatcttcat gatgtttaaa tgcttctatc atattttcat
     4621 cctggctcat atgtgcaaga actcttagct ctacttgtga gtaatcggca tcaaccaact
     4681 tacagttatc atctgcaata aacacttttc ttatttttct acccatttcc atttttatag
     4741 gtatattttg aagatttggt tctgtagatg aaattcttcc tgtagtcgtt atagtttgat
     4801 taaatgatga atgtattcta ttactttctg gatttattat atttaaaagt ccttcaacgt
     4861 aagtagaatt taactttact atttgtctat actcagttat cttatctatt attggatgct
     4921 tatctcttaa tttttctaaa acatctgcat tagttgaata tccagtttta gtttttttaa
     4981 ttattggaag ctctaatttt tcaaataata taacacccaa ttgttttggt gaatttatat
     5041 tgaatggctc tcctgataat tcataaattt ccttttctaa tgtagctatt atttctttaa
     5101 attcaacact caattcatcc aatatacttt tgtctacttt tataccttca tactccatat
     5161 gacctaagac ttctactaaa ggcatctcta catgataaaa aagaccatcc atactcatat
     5221 ccattagact tctttccatc ataggaactg tttctttaac tgtgcatatt atttgaccca
     5281 tatactctgc taaatcctca aattctaaat cctcatagtt tttagattta acaccctttc
     5341 ctaataattc ttcttttgat ttaacctttt ttgataaata cttcatagct attgcactac
     5401 aatcatatga ggtagatgaa gttgaatcta ttaagtactc agctattgtt atatcaaaat
     5461 acatattttc taagtttata ttatatggtt ttaaagatat ataatcatct tttaaattat
     5521 atccatactt tttaatttca ttatttgaaa atatactatc taactttgac acttcatttg
     5581 aatctacata atatattttt tctccatcta cacttataaa aatatatatt atattttttt
     5641 caagtatatt accttgtcct ctaacagttt ttaaaataac ttgtcctttt ttgtttactt
     5701 catctataaa tttttctata tcttttaatt ttaatatttc tattttttct tctacgtgtt
     5761 ctgtgtcatc tgaatcgacc aaatcaacta atcttgataa gagacttgta aatccaaagt
     5821 atctaaattt atctaaaagt tcatctttat cataatcgcc aaaaatcata ctatctaaat
     5881 ccacttctat tggcacatct cttataattg ttgcaagtct tttactttga atagctattt
     5941 ctttgttttc ctctattttc ttttttacac tacctttaag cttatctgta ttctctatta
     6001 gattctctat actagaaaac tcttttataa gctttatacc tgttttttca cctattccag
     6061 gtactccagg tatattatca gacttgtctc ccataagacc ctttaaatcg ataaattgtg
     6121 ttggagtcat ctcatatttt tcaattacag aattaaagtt gtattcttct acttctccaa
     6181 cccctttttt cgttataagg gtagttgtat tatctgaagc aagttgaata gcatctttat
     6241 ctccagttac tatgtatacc ttatatccat tttcttctgc ttttttagaa acagtaccta
     6301 taatatcatc tgcctcatag ccatctattt ccaatctatt tattctaaat ttatctaaaa
     6361 ggtcttttaa tggctgtaat tgttctgcca gttcatctgg cattcccttt cttccagcct
     6421 tatactcttt atattctaaa tgtctaaatg taggtgcttt tctgtcaaat gctacactta
     6481 tatgtgttgg tttatatata tctatcattt taaatagcat tgttgtaaat ccatatattg
     6541 cattggtctt taacccttct ttattattca tttctggaag agcataaaaa gctctattta
     6601 ttattgaatt tccatctatt ataattaatt ttttttccaa attatatcac tcctaaaaat
     6661 tatctatttt tgtaacatat atattatata ccaatatact gttatttaat attcccataa
     6721 taataatagt atattaaaat taaatatgtt gcaaacaata tatttttttg ctataataat
     6781 atacatgaca tgcccgagtg gtggaattgg tagacgcagt ggactcaaaa tccaccggac
     6841 taatactccg tgccggttcg agtccggcct cgggcaccat taaaaactcc taaagctatt
     6901 tattcaatag attaggagtt tttttatttt tgatttaaat tttaagttat tttttgaaat
     6961 tactgtgtct ttttaaaatt tatataatta tataaattta tcttctagca ttaatttaaa
     7021 aactaattta tttttatata attttctatt ttatatcata acttattaca actttttatc
     7081 taatttattg tatacaacaa cactacaaat aaattgaaat aaaataagca atccaagaac
     7141 aactaaaacc cacttgctta ttaccattac agaaaataca ctcaatatag aaacaataaa
     7201 tataagaatt acaagaacat aggacatgat ttgattagtg atacgtccag ctttatcacg
     7261 taacataatt cttctttcat cattcatctc tatcctttga ttttttatct tttcttcata
     7321 atctactgct ctttcaggtt tactccaata catatattga agaatcatac atacacctgg
     7381 acctaaagca gctccagata atccccaaat aaaagcctca aatgaaaact cagtaaatgt
     7441 agccaataaa acaagtaaaa ttccacaaat aaaatatata attccaacat ataacatatt
     7501 ctttttcatt tttactcctc ctcataaata aatatttctt caatactcat atcaaagtat
     7561 ctagctatct taaaggctaa gatgatagaa ggattatacc taccattttc taatgaacct
     7621 atagtttgtc ttgatacttg caaaatagaa gcaagttctt cttgttttat gcctctttct
     7681 tttcttattt cttctaatct atttttcaca tctatccccc tttaatatct caactacgga
     7741 aagtctactt tccataatta tacaactata ttccatttat gtaaagctta ctttccatat
     7801 ttacaaaaaa ataacagact attttcagcc tgtaatttta gaatttcata ttgtttatta
     7861 attttaatgt tatttattaa ttccatatta tttattaatt ttaacttggt ttctgaattg
     7921 gaatccaaac ttctgtagta taatcaggag cattgatatc gccattggga taaagttcaa
     7981 tatctggtaa attagcatac acatacccag aacttggtaa ccactcagta ataattcttt
     8041 tttgcaattc ctgtattgcc agagcatttg gcattggaga aacacattca aatactgccc
     8101 atgtgcctga tggtattaag tattcatgag tatcttctaa tacaggctta tctgttggag
     8161 cagcaatata ataatctaaa cccttactat ttgtacacac actaactcca agcaatccac
     8221 aaggttcgtt atttattaac tctgaaagtt tatcaattgt tccatttttt atgcatttag
     8281 cccataactt aggaactttc tcaaaacact cttctacaat catgttcata ctttctttta
     8341 taccaacaat acgaaaccct ttcttttcta caatacgata ttgcatttct atatttcctt
     8401 ttacataaat gcttaatgtc atcttaggat atgcttttaa aacaacaccc tttgctctag
     8461 ctgcagatgg agaaatgtta tgtatatttt gaaaagctct attaaaagat gttggtgaat
     8521 cgtaaccata cttctcagat agttctaaaa ttttgatgtt actgttctga agttcaaatg
     8581 cagccttagt catacgtctt cgtctaatat attcagataa tggtacttct gcaatatatg
     8641 aaaacattct ttgaaaatga aaggttgaac aacatgcaat ttttgctgct tctgcataat
     8701 ccactccact tttgagattt tcttcaatat aagtaatagc ttgatttaat ttatttatcc
     8761 attccatttg atattacctc caagcaaatt atacagttta ataaaataat atacctctct
     8821 attcatgcac aaaattgtaa gcatttaaat ttataagcat ttttcaatca atacaataaa
     8881 tataaaaatt gtattataca ataaaaaagt tcaataacat tatattataa aatgttattg
     8941 aactttttat aaaaattaat ttaagtgtag ttaaattaag tattacacta tcttatatat
     9001 tacaatatcc tactatctta tagtcttctg gcactttata atctttgtct ggagtaccta
     9061 aattccattt taaatcaaac atagttgcac ttattattgt tgcaaaataa agcataacta
     9121 taccaatgtc tattttttct tcatataaat tagtatgacc atcttttcta acagctaaaa
     9181 ctacagtgcc tccatcaact ataaatctcc atggttgtct atttaatgta gatggagcta
     9241 atcttgcata gtgaaatgca tcaagaagtg ctctttcttc taagatttct atacttgcaa
     9301 catttcccca ttcgttcata taaacaacat cttctacacc tagtctagta gaccccttat
     9361 ctgttacaac ttgcatatca gattttgaat aattttgccc agtttttgta gcatttaata
     9421 ctttagcatt acttacattt tctccatatc caatagctat aattcctgtt acctctttat
     9481 ctgaattaat tccaagtttt tcttttacct tttttccatc ttcaaaagta acccaacaag
     9541 aaccaattcc aagttctgtt actttaaaga ttatactttc tcctatgtac cctgaatttt
     9601 caatgtaatt atcttttatg tcagataata ttataatata acttggagaa tctatcatat
     9661 ttcctttgta ccctgctact ttatctaaat tattaaaaac ttcacttcta ttcataattc
     9721 ttacatcaac atctatttct ggaactagtt tcttacaaga ttctgagtat ttcttaattt
     9781 cactaagata ttcttcttta atatctgtgt tcttatattc tctaacagac tttctattta
     9841 atatgattgt tttataatcc ataattttat cctccccaaa ctagtattgt cttaattata
     9901 tacccattaa tatattattt tactcattta tttaataaat aaagattaca attttgtaat
     9961 tattatttat taataatatt ccacttttac attaataatg accagttgtg caaaatccat
    10021 tgccaaccac caaataaaaa catattataa aggtaatata aaataacaaa caaatcttaa
    10081 caaaaaatta aacttttttt acacgaaaga aggcttcccg ttgccttctt tatttttata
    10141 aaaaaataag ctgtttctaa aatgatttca catttttgaa acagccaagt tattctataa
    10201 tgacaactat aatttgagga tgtgagggag agttgtcatt ataaatttag ggtaattata
    10261 ttatgtttta agctagttct ttctatattt atataataca ttttatatat gaattttata
    10321 tgagattggt ataaatgata tatgaaatta catatgttta tattatatta aattttatta
    10381 ttacattttc cacaacaata ctcatatgtc tccacattat ttggaatatt tacaaataca
    10441 aattctttgc agttctcaca atactttaat atagatatag ttccacaagt gtaacattca
    10501 aatattacac aatcctcatt ttcctctact atttcaaact ttttactatg ttcaccacac
    10561 tctgggcact tgtgattatt tacattcata aatactcctc cttattagta tagttacatg
    10621 tcaaaaactt aattaatgta ttctatatta cttattatat attctataat aaatcatatt
    10681 ctaatataat aaaacaaatt aaataaaaaa aaaccataaa ttttaaattt atggattaat
    10741 tcttgtaatg ataactctat ttgaggatgt gagggagggt tatcattaca atagggtatt
    10801 atattatggt ttttaagcta gtctttctac acctcatatg atacatatgc tatatgaatt
    10861 ttatatgatt tttacataaa tattttatga aatttaaatt ttttagtcat tttgaaattt
    10921 gtaaaaagtt ctctaaaaat ccacagcatc ataaacagcc aaaatttaag tagaacatca
    10981 taaaaaaatt tgtccaataa ttttaacaag taatctcacc tcatcataat tattgacaca
    11041 aacttaaata aacaatctat aaatcttcta taaatcttaa actttccccc tattgataca
    11101 caatttctag tttgtcaatt ttattttgca tttctaagaa aatttcttca acagaattgt
    11161 taaatatatt gcctaaatag attcttttat ctttttgaaa tgaataagat tttacactac
    11221 catcagaaga aatatataaa tgactccact ctgctccata ttttgaaaac ctctcctttg
    11281 tatctttaaa cctcatatct tctattttta ttgctttttc tctgtctcct cttactacag
    11341 tgtgacccct ttttattaaa ctggttattt ttatatcttc ttttaattta atacgtgtat
    11401 catcattttt tgaatatact ttccatatca ctttatgacg ataattatct aattccttta
    11461 ttaagtttct atcaatagta aaactatctt tagttaccac aattaaatca aaatggtcta
    11521 acatatattg aaatatttct ctcatacttt tattttttaa aatatcctca ccaataacct
    11581 ctataaatct acagcctttc tcatacatgc agttcaataa acttgtgatt tcagaaaaat
    11641 aatcatttgt attattatct ccatctattt ctatagtaaa gtacaaagga gtaatgtatg
    11701 atttatttcc tgttatctca aatacacttg atagtgtctg tatatcatct tcaaacgtta
    11761 agtatacatt ctcataaaac ataaaaaatt ccataacttt ttctttcata ttatggatat
    11821 ttctatatct attggataat atatttatta tatcgtcagt agtatttttc ccatcacatt
    11881 cagccagtat aacaatttgc ttccaactta agctatccac atttccgata ctttttttat
    11941 atccatcttc ttcatcataa ataggaccaa ttataatgtt ttcttttggc cacagatgaa
    12001 ccctagttcc atcttttaat ttcggatatt tattttttac agactcataa aatctacttg
    12061 tatcacccat gattctttac taccccccat agtttaaata ttctatgcta aaaatagcac
    12121 tttattttta ttaattcaac aattattcaa aattggtcat tatatatgtt taaatggatt
    12181 taacatcaac cttatttata tgttattgat gtttatatat gttatataaa atcttatttc
    12241 tcatacttca atgtaatctt aactatctct ggtttacaat taagcctaat taaaactttt
    12301 gagcctccta agcctttatt taaaatcata gtgctattat ctttttcata ttttccccag
    12361 tcatacttag gaaagaactc tctatttgga gataatacac ctccaacaaa aggtattctt
    12421 attatacctc catgaacatg tcctgctaaa accaaatctg ctccccattt tgcatatttt
    12481 tcaaagaaaa atggtgtatg tgctaaaaga atgttatatt cattttcatt aactttacct
    12541 agattgtctt ctacaaaatt gtcacttagt tctaattttt tgttattagc aaataaatat
    12601 ggatagcatt ccaatggtat aattaaacca tataaattaa gatggctatt acctctatat
    12661 attcttacag attcattttc catatttatt attttctttg aatatagttc cttaaaataa
    12721 tccttatata gatgtttata cttttttatt aaagattttt gttcatgatt tccaattata
    12781 tgatatactt catatttttc aaccaaatta tctattaaat ctaatgcaac ttgaaaattt
    12841 ttattttctc catctactaa atctcctgtt ataaatataa catctgggtt taatgattca
    12901 attttatcta tcaatacctt attattttta tcaaacacct tattatgtaa atcactcact
    12961 tgaactattt taaagttatc aaattccttt ggaatttttt tgttgaaaat agtatattct
    13021 gtcgtttcta tatgctgaat ttcatactta tacagaatat acaataatat taatactaat
    13081 actgtcattg aaacctttat aatcatattc tcttcctctt tattctattt tgttaatatt
    13141 attataatgc ttttatgttt tttttaaaac aataacatca tcacatacat ataaatcttt
    13201 tcgacattat aaaaataagt tatccttttc taaaattttc atttatttta gaaaaggata
    13261 actataaaat tatctataaa cctctatatt gtgaaatata gtttttcctt tatttaaatt
    13321 tttatatata tgctctaaag ttccatcaaa cctaatagaa tgtccagact ctaaaatata
    13381 agttttccca tccacattaa ggtcaagttt accttctatt acgagcacat attcttctaa
    13441 aacatgacta tgtgtagatg aaacatgagt acagttttcc tcaagctcaa ttataaaaag
    13501 ttcaaacctt ctttgagggc taaaagggta tattggatat aacttcatgt tccctttttg
    13561 ctcaacaata ggctctactt cttctttttt tataacctca taggtatttg tattttcatt
    13621 taatagtgta gtaaaattta ttttaaatcc agatgaaatt ttccataaag tagatactgt
    13681 cggacttgat tcacctctct caatctgtcc aagcattgct ttactgacac ctgtcatctt
    13741 tgaagctttg tctaaactta tgcccatttc ttgtctcaat aaccttaaat tttctcccac
    13801 attaggaaat ttattatcca tgttaaaatc ctcctgatag ctatttgtat tctatgattt
    13861 ttactcctgt aagttctact atctctttta attttaacat atgattgtta caaatttcat
    13921 caaattcata tatataatct tcaagcccta atgccttata tttatcgatt ccaaatttat
    13981 gatatggaag taactccatc ttgcctcctg gaacattttg tttgacaaat aatgcagtat
    14041 ttcttatatt ttcttcacta tcatttactc cataaataat tggcactctt ataaccatag
    14101 atttattcaa ctttgaaagc ctacatatat tatctaatat aattttatta cttactcctg
    14161 tatattcttt atgaatatta tcatccatag attttatatc tacaaatatg tggtctattt
    14221 tctcaaatac atcatttacc ttattccaat caaaatatcc acaagtttca attgcaatat
    14281 ttatgccttt atcataaaaa atatcaacta attctcttaa aaaatcaatc tgaagtgtag
    14341 gttcacctcc agaaaaagtt atccctccat tagattcaaa gaaaaatata aaatcttttt
    14401 caacttcctc tactatttct tcaacactca tttcttcggt cattatacaa atggcatctt
    14461 taagacatac cttgatacac tctccacata aatcacattt attatttatt tgcgttctat
    14521 caaatagtga acttatattt tgaggacaaa catctataca tctattacag gatacacatt
    14581 tatctttcat aacacctaat tttactatgt tactccagga atctggatta gaacaccact
    14641 tacattttaa tttacatcct tctaaaaata tagtagtcct tattccatct ccatcattta
    14701 ctgaaaaatg ttgtactttt acaactctac cttttttact cataatctac ctcataatta
    14761 aaaattgcta tgtgaatttc tagctattat agcatcttgc atttctcttg ataaattaac
    14821 aaattgagta ctatatccag caaccctcac taataaatct ttatattctt ctggattttc
    14881 ttgagctttt aatagagtat catttgatat cgtattaaat tgtacatgga atcctcctaa
    14941 agcaaaatat gatttaatca tagatgttag atttctaagt cctctttcag tttccaccaa
    15001 atcttgattc agtcttaaat ttagaagtgt tccaccagta tgaacatcat gatttatttt
    15061 tgatgcagat ttcattgctg ctagtggact tgttgtatca gtaccaacaa atggagaaac
    15121 tccctctgtc aaaggttttg tcgcttttct tccacaagga gttgcaccta ctatttttcc
    15181 agttggtaca tagtttgata tacccataaa tgcactatta aatttagagc caaaaatatc
    15241 cttgtatttt ctagtttcag tataataaaa atcagaaact tcaattgcta atgaatctac
    15301 ataatcatta tcatttccat acttaggaac atccaacgca agttttctta attcttcata
    15361 accttcccaa tcattattta gtgcttttgt taattcttct aacgtagtca ccttatcctc
    15421 aaatactaat ttttttatag ctgccaatga atttgaaact acacctaacc ctataccagt
    15481 taatacagga cctatattat attttgctcc tcctttactt aaatcaactc ccttatccaa
    15541 acatccatct acacaagtag ataaaaatgg tcttggtacc atttctttat gaatttgttg
    15601 agcaacagta gttcctataa cagaactttt tacaagatat gctaattgct ttaaaaactc
    15661 atccttaagt tcttcaaaag aagtaaattc tgtgatattt tttgtatcta accccatttt
    15721 ttctccagtt aatctactct taccttcatt catagcatat tctaaagctg ctgcaaaatt
    15781 gatatttacg gctgaagtcc actgtcctgt ttttctaaaa tgaggaacta cacaaccaca
    15841 gttactccaa tctctagcat cttctgcatc atatccatct tgtagtaaca tttgtgcccc
    15901 tgcactatcg ctgtgtatag cagggaaacc agttccttgt tttaccaatt tagcaactgc
    15961 cattacgaaa ttgtctggtg ctccttgact cactctaaca cttaatcctg gctggtgagt
    16021 ttttacactt tctgtagcct ttaaacacat atatgagata tcattcgttc catcagtacc
    16081 atttctcttt ttaccaccaa cagtaagatt ttggaattga ttatatcctg caaagtaatt
    16141 tgctgtattt gctgatatag tccataccca ttctgataac ttaatccaaa gtgcttctat
    16201 caattcttga gcttctgatt cagttatttt accttctcta gcatcatttt cataatatgg
    16261 atacatatat tggtcaaatc tacctaaatt taatgccaat ggattttctg atagaatacc
    16321 acctaattga acaaaccata caaattgtat tgcttcataa aaattagttg gagggtttgc
    16381 tggtacattc atacatatct ctgaaatttt tataagttct aactttctct tttcatctgt
    16441 ttcttgctta ctcatctctt ttgcaagatt tgaatatctt tgtgccaatg tcataatacc
    16501 ctgtgcaact atagaaacag atttgtaaaa atctatcttt tcaatatttt ctgaaactga
    16561 tatatctaat tctttaattt ttttatctgc atcttcctta atccctttat atccttttac
    16621 aaacaatata tcttgataat ctggtgttac ttcaccaact gcctgtctcc acttagaatc
    16681 attatctatt attcctgtat ctactaatat cttagctgta tcttctggga ttcttgctaa
    16741 gaaaacttct tccaaagatt ttcccttcca atatggaaat atttcttttc ttataaattc
    16801 tatttgttct tttgatatta catatgggtc ttgagttctt ttatcaaaag tatccatttc
    16861 tttgtctacc cattgccaag aaaactctgg tgtcaatatc cctgttcttc taaattttcc
    16921 tgctgtacct actattaatt cattttcaaa gatttttata ggaagttctt tacatgtctc
    16981 caaaaaagct ttagctcttc ttatacagat agcttctccc tcagtttttt tatgtgattc
    17041 tgtaaaaatt ctagcccttt cacaacatat cataggttta gaattaatat attcttgtct
    17101 aaatgcttct attctattag tttgactcat ttaaaatccc cctttggata atatatacta
    17161 catttttgta tgttataaca tactttaatt atattatact gtcctttagc ataaaatgga
    17221 agcaaaataa cttatttttt ttcatctacc ttgaacacct atatattaat actttttttg
    17281 ttaaatttat aaaatctaaa aaaattttct atgatatatt ttctatattg ttatagttat
    17341 ttctaatata ctaatgattt gaaatatcaa atttgtatac tagacttagt tttaaaacct
    17401 tagttgattg tttaactttg ttaacttcaa catgtatatc cttcatataa ggaagctcta
    17461 caacacctac tcccttatta aagtcttctt caaccataga aaaaatttct ttctcagtta
    17521 agttcgtatt agtcaatgat ttaaataaat tgtatataaa tttaatatat ttatcactct
    17581 cagataactt atcatcttct ggatactctt tagcacagag aatatctatt gttacttttc
    17641 cttctttatc gtcttcagac atagaataga ataaacttcg aatatttttt tcaggtggtt
    17701 tttcagcacc atttatagat actacaaagc taccatctga ttttgagtta gtaatactat
    17761 aaccactgtc tattatagct ttcgaaaatc tttgagataa atcttttttt acttcttctc
    17821 tacctttcgc atcattaatc ttttgtgagt aattattaaa tagctcgata gattcatctt
    17881 ttttatcttc aacttgttct ttcaatgaat ctttactaat ttgttctgag ctacttaagt
    17941 tcttatcaga tacaggagac atacacccta ccatcaatgc taagctaaga tttaaagcca
    18001 caatgattgc cactctattt atattccttt ttactttcat acacaatcct cccatcacac
    18061 taaccgctct aaagtattat tcattgcact agaatatatt tatatttttt ttatattaaa
    18121 gatttattat tcaatacata tcaccttcct atccaattct tataacttac tattagtaaa
    18181 attataagct ccttaataca gaaataagga tgttatcttt tgttattaaa agataacatc
    18241 cttatttttt tgtatcaata catatgtccc aactggtaac ttaatcgccc caactgtcag
    18301 tatcttggtc caatggtata tatatattta gtataaattt atcgatacta ttttcaaata
    18361 ataattctcc attatatttt tgtaaagaag atttaatact ctgaatacct attccatgta
    18421 caaacttatc cattttgttt gttaacaata tatttttatt aaaagtaagc tgatttagtt
    18481 tactattttc acatttaagt acaaaaaatg accttgtcat agttccttta attctaatat
    18541 atttattaac attttcatcc actattttat cacatgcttc tattgcatta tccaaaatat
    18601 tagaaaatat gcttgacaca tctataggtt ttacaaaatt tacttttgaa aaatttatag
    18661 agcaagtaaa tttaattcct ttttttaaac acatttcact cttttcattg attataatgt
    18721 ctaatatcat atttcctgta ttatagatat atttaaatgt ttttatttca tcttttaaat
    18781 tgttaacata ttcattaatt tctttgctat tgtttctaag gttgtctata caataaatgt
    18841 gattatttat atcgtgatat agtttcttaa ctcgcatatg agattcttga atacttaaat
    18901 aatgcgcata ttgcatatct aacttatttt ttattaattc agcttgagat tttaatctat
    18961 tctcttttat tatttcttta aacaataata taagaaatat actactagct cctaccaatt
    19021 ttggattaac aaaattatca aaatagtatc cttctgaatg taatgatcca aaccgttcta
    19081 atttttttaa tacataattt acaagcatat tcattgtatt aactaataag gcaaataata
    19141 aatatacata atttctctta tcttggctaa ttaattttat agactttcta aaaaagctaa
    19201 atataagata tataagaata attattaaat tactatatat ctgaattttt atagattcta
    19261 agtgaggtac taataaagat tctttagatg aaataacatt acctgtaatt gcaaaaaata
    19321 gtattctata tataacagca tccaaacatt tataatatac actaaataga taaaagaaaa
    19381 tggaatactt aatataaccc atcttataaa aataattata gtatcctccc caagcaataa
    19441 atgctaata
//
LOCUS       DAFKMX010000063        19356 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFKMX010000063 DAFKMX010000000
VERSION     DAFKMX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.063"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            169..573
                     /locus_tag="LZB08_001790"
     CDS             169..573
                     /locus_tag="LZB08_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBM7315294.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(830..1825)
                     /locus_tag="LZB08_001791"
     CDS             complement(830..1825)
                     /locus_tag="LZB08_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBM7315295.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(2124..2960)
                     /locus_tag="LZB08_001792"
     CDS             complement(2124..2960)
                     /locus_tag="LZB08_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBM7315296.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(3448..4011)
                     /locus_tag="LZB08_001793"
     CDS             complement(3448..4011)
                     /locus_tag="LZB08_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315297.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(4109..5038)
                     /gene="glsA"
                     /locus_tag="LZB08_001794"
     CDS             complement(4109..5038)
                     /gene="glsA"
                     /locus_tag="LZB08_001794"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBM7315298.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(5932..7599)
                     /locus_tag="LZB08_001795"
     CDS             complement(5932..7599)
                     /locus_tag="LZB08_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBM7315299.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(7673..8428)
                     /locus_tag="LZB08_001796"
     CDS             complement(7673..8428)
                     /locus_tag="LZB08_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBM7315300.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(8438..8974)
                     /gene="lepB"
                     /locus_tag="LZB08_001797"
     CDS             complement(8438..8974)
                     /gene="lepB"
                     /locus_tag="LZB08_001797"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBM7315301.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(9140..9676)
                     /gene="lepB"
                     /locus_tag="LZB08_001798"
     CDS             complement(9140..9676)
                     /gene="lepB"
                     /locus_tag="LZB08_001798"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBM7315302.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(9776..11086)
                     /locus_tag="LZB08_001799"
     CDS             complement(9776..11086)
                     /locus_tag="LZB08_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBM7315303.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(11165..11881)
                     /locus_tag="LZB08_001800"
     CDS             complement(11165..11881)
                     /locus_tag="LZB08_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBM7315304.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            12126..13397
                     /gene="sleC"
                     /locus_tag="LZB08_001801"
     CDS             12126..13397
                     /gene="sleC"
                     /locus_tag="LZB08_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBM7315305.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(13905..14561)
                     /locus_tag="LZB08_001802"
     CDS             complement(13905..14561)
                     /locus_tag="LZB08_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315306.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(15834..16244)
                     /locus_tag="LZB08_001803"
     CDS             complement(15834..16244)
                     /locus_tag="LZB08_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315307.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(16460..17842)
                     /locus_tag="LZB08_001804"
     CDS             complement(16460..17842)
                     /locus_tag="LZB08_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBM7315308.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVEVGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(17853..18212)
                     /locus_tag="LZB08_001805"
     CDS             complement(17853..18212)
                     /locus_tag="LZB08_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7315309.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     ISSDEIAKLKQIVEKLK"
     gene            complement(18417..19196)
                     /locus_tag="LZB08_001806"
     CDS             complement(18417..19196)
                     /locus_tag="LZB08_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBM7315310.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         6558 a         3529 c         1873 g         7396 t
ORIGIN      
        1 cccatcatat atcaaatttt aaaatttgta agcaaaatac ttatcttata ttactacatc
       61 taaagtggaa aattttacaa acaagttctg taaaaacact ttaaataaca atttgctttt
      121 aaatcatata cagattataa taaatactgt ataaggatgg tgaaaaattt gattaaaata
      181 acaattccag gtagacctat tagtaaatct aattttaagc ttcataacat aaatggtcaa
      241 gcatggatgc cttcaaaggg aaaatattct aagtatcttg catacgaaaa catgattgca
      301 ggttttataa accaacagta tcaaggggaa actgttgaag aaaatctaat aacagtcttg
      361 aaattattct tcccaaataa gagaatgggg gatttacata attatccaaa aagtatttgt
      421 gatggtattg aaaaaagtgg aataataaaa aatgataaac aacttaagcc agtacttcta
      481 tttgatttta tagataaaga taaccctagg gttgaaattg aactttatcc agtatctaaa
      541 tacgatatat cctataatat ttttgaaaaa taaagatttt atgtgttatt cgacattata
      601 ttgatactcc agtatttgaa actatactaa tacaacaata ataattgtgt tatgtctata
      661 ctcaaacaaa tgtttttaaa aagcattaag caaatattta ataagtatta aacaaatatt
      721 aaatttatat aatagctata actatgtaaa tttaatattt gtatataaga ccacatctaa
      781 taaaaagaaa tcggagtaaa tttaatacac cgatttcaat aaataaaatc tatcctagct
      841 tttcaacaac agtcttaagc atttctctta taggaagttc atatggacat ctactctcgc
      901 aaagcccaca gtcaatacac tcagtaggtt taacatccat aacctcatat ttttctttag
      961 cccactcttt taatccatat ctagtataat aaccttcaca taaaaaactc aatggtatat
     1021 ttattcctac agcacaaggc atacaatatt cgcatcttct acagaatttt ttacctaaag
     1081 aatttcttat ttcttcaatt tccttattat ctttttcatc tagcacaaga ttttcaagta
     1141 cagcgacatt ttgtctaacc tgttcaacac tctccatacc tggtataact acatctatgt
     1201 aatctttaga tagtatatat ttaatagcca gtgttgcatt gtctaaagca cctccagcta
     1261 aaggtttcat tactattatc ccaattcctt tttcatgagc ttttttaaac acttcatctg
     1321 cttgtccttc aactatattg taagggaatt gtatagtgtc aaattttcca tcttctatag
     1381 ctttttctat agtatttaaa ttgtgactag ttataccaat atgttttatc ttaccttgct
     1441 cctttgcctc taaaagggca ctataagcca ttttatcttt aaaaagatta tcatactcct
     1501 cttctttaac attatggaat tgatataaat ctataaaatc agttttaaaa ttatttaaac
     1561 ttatttcaac atctctcttc attgaatcat aatctctgga catactttta gtagctaaga
     1621 aaaatttatc tctttttcct tcaatggcaa ttccgattgc ttcctcactt attgtatacc
     1681 ctcttgcact gtcaataaag tttattcctt gtttttctaa ctcatttata actaagttag
     1741 tatcatcttg agtaatcctt tgtattggaa tgcccccaaa gccaactctc tttattttca
     1801 tgttagtatt gcctaaattt ctcatatctt tccccaccta acttttaata attttttata
     1861 taatataaat atgtcacctt taattataca acaaattatg agaatctttg caatatattt
     1921 acatactatt cataatataa ttatatttta tatcaatcta ttcataatat aatcatactt
     1981 tatataatca tactttatac ccagttaatt tgtatacaat ttttgttatt actatccatt
     2041 aatttaaatt atcacatttc atgattgtat ctccacccta attatgttga gggtggagta
     2101 tttcacatat ttttaataaa ggtttattta ctagatagta tttcttttat cattttaagc
     2161 tctctcttgt gaccttcatc atcaaaagga gtttctaaga aaaatggtaa atgttttaga
     2221 ctaggatgtt ccataaaatc aattaatgct tttaatccta tctttccttc ccctatagga
     2281 gcatgtctgt cttttttgtc tccaaatggc atcatactat catttagatg gacagttttc
     2341 aatctttcta tgcctataac cttatcaaat tcttccaaaa ccccatctaa atcattcact
     2401 atatcataac cagctgaaaa aatatgacat gtatcaagac acacacctat tttttcttta
     2461 tgctcaattt tgtctattat actttttaat tgctcaaatt taaaacctat ttcagttccc
     2521 tttcccgaca tagtttcaag aagtatagtt atattttcat caccctttat ggcattgttc
     2581 aatcctttta ctattctatc tataccaaaa tctacaccac cacctacatg acttccagga
     2641 tgaaaacaca tgtattcaat tcctaatgag tccatccttc ttatatcctc atctatcacc
     2701 cttgttgcaa attcgtatac atcatccttt gtaccaccca gattcatagt atatggtgca
     2761 tgtgcaagca gtggtgcaaa attattttct tttctaattt cttgaaattt aattatatct
     2821 ttttcatcaa attctttagc atttcctcct cttggatttc tactaaaaaa ttgaaatgta
     2881 tttgcaccaa tatatacagc tgtctcagcc gcattagcaa agccttttgc tattgaaaga
     2941 tgtgttccta atttaatcat attaatctcc ttttatttgt actacgactt gtactcaatt
     3001 ttaattttga actactccac cttaactaca tttagccaac atatgaagat ggagattcct
     3061 atttgatagt actttttcac taaaaattcg taagctctaa gggatgttct atcccaacta
     3121 atctcattcc tcttttaaca tatttctagc attgatttaa tttatattaa tttttaattt
     3181 taatatttac actttaatta tacccatttt aatcaatttc aaatattata atcttacata
     3241 ctttgtatat tatctaagca cttcttagat agtgtataca tcttacacta tacatattat
     3301 ttacaaatac aataggtata agaaaacaaa aaaatagaga ttaaaaatca ttgttttacc
     3361 cataccaaaa actatttttt gattattaag taaattttga tgttcaatct ctaatattat
     3421 atctttaatt aatccataat taattctcta actaggacat tcctcctcaa gagtaggaac
     3481 tataaccata gctatatcat acttagaacc ttcaacattg tgttgtcttt tatttaaaat
     3541 ttcatctatc tttgacttca tagtgtcact aagttcaact aattcatcat gagtaagaga
     3601 aatatcatga taatcaacta tattttcatt ttctaaaaca tcttcctctg ctgccttata
     3661 aaaaacttca ctcatatttt caaattttga tatgtacaat tcttcttttt cttccccatt
     3721 gtctaaagaa aaattaagta tcctattccc tataaccaca ttttttgcac ttggataata
     3781 gtacttttcc acaatccccg actttatttt ttcctcaact aattctaaaa taccaacttt
     3841 gtataacatt tttatatggt agtttatttt tgcatgaggt tcttccaata attgagataa
     3901 ttgttttgct gacaatggcg atttgtcaaa tgcttttaga atatctattc tacgtggatg
     3961 cgcaatagcc tttatgcact ccaaatctcg taataccaat acctctttca ttgacccctc
     4021 ctaaaaaatt tatttaattc atatttcttt acaatttaat tatatattaa attatatgtt
     4081 gttgtcaaaa aaatcctttc ttaatatttt aaaaaatatt taatttgagt ttatttgaca
     4141 actcctctaa aactttaact cctgcgacgc tgttaccttt tttgtcaagc gcaggtccat
     4201 aaacaccaat ccccatgcgt cccggcacag aagccattat accaccccca actccagatt
     4261 ttgcaggaat gccaacttta atagcaaatt ccccagaagc atcatacatt ccacaagtca
     4321 tcataaatgt cttgacaatc ctagaaacca tttcactcat taaatgctca ccattttcta
     4381 tatctactcc ataatttgca agatttattc caattctcgc taaatctaca gaatctatct
     4441 caattgagca ttgtttaaaa taaaggtcta gaacatcttc tacattccct ctgataaatc
     4501 catcactttt caataagtaa gccatagctc tgtttctatc tccagtcatt ttttcagact
     4561 tatagacatc atagtttatt cctatattat cattttttgc taactttcta aagaaactaa
     4621 gcattctttc ttctttttct tctaaacaac tcccatttat aagggaagtg gttactatag
     4681 cccctgcatt tatcattgga ttacagggtt tttttgtgtc attaatttca agtttcatta
     4741 ttgaattaaa tgggtcccca cttggctcca ttccaacgtt tgaaaaaacg tcttcccaat
     4801 cattatccat aagtgccata gcaagcacaa ttggttttga aatactttgt atagtaaact
     4861 ttgtgctaca gtttccagca ctataaagat tattttcgct atctatgata caaattccta
     4921 aatcatttct tctagcgttt tttaattctg gtatgtaact agcgacttgt ccataattag
     4981 tatacttctt attagaactt ataatttctt ttaataatgt ctcatctaac gtattcatat
     5041 gcacctctta tattatgtta tatattctat tttagcacaa gtttatacta taataaaaca
     5101 atgttaaaaa aaattatact ttaaaattat tttctacaag aatcaattca tttttcaaac
     5161 aatttaaata gtttaaagag cttaataatt taataattca ataatttaac aacctagcaa
     5221 tttagtaatc taataatcca gtaattcaac aacctaataa tttagcaatc taataatcta
     5281 actatctaat aatccagtaa cttaacaacc tagtaattta gtaattcaac aatctaacaa
     5341 ttcaataatt taacaaccta gtaatctaac tatctaataa tccagtaact taacaaccta
     5401 gtaatttagt aattcaacaa tctaataatt caataattta acaacctagt aatctaacta
     5461 tctaaataat ccagtaactt aacaacctag taatttagta attcaacaac ctaataattc
     5521 aataatttaa caacctagta atttagcaat ccagcaatct aataatccag taatttaaat
     5581 ttttataaca ccaaaaattt ctaacccaac accttttact cttttactct tttactcttt
     5641 tactctttta ctcttttact cttttactct tttactcttt tactctttta ctcttttact
     5701 cttttaaaaa ataatcacat aaactaacaa aatattaaac aaaaaagtaa aatttaaaat
     5761 aaaaagcttg cttcatacaa aagtataaaa caagcctttt tatatatcaa ctcaaacttt
     5821 aaaatctgta tcaatttaat tctccacttt acacttaata tacttaacat aaaccaatca
     5881 cactacttgc tatgccattt aatcttcata aaacaatcta tagtacacac attaatcaac
     5941 tattttactt cctccaataa attccctaag aattgaaata cctggtatgt cagaagtatc
     6001 gtccaattca acaaaatact gcaaaaactt tagtaatcta gtagcttcaa tatgtgcagg
     6061 attatcttcc tttatttctt ccaacaaagg tttcgcaaat cgttttaaaa ctgccaattc
     6121 atatacacaa gcagagttaa atatagcatc tgcctcctct tggtatggga atatattctt
     6181 cttttctcct cttctaactg atgcccattg taaaatagta ttatcagcac tatatcctct
     6241 aaaattatag tctctaacaa ttcttcttat cattctcaaa tcagcagtag ggattcgatt
     6301 atggtcatcc aaatttattt gagtaagggc acttatgtat atcttaaatt tatcttcatc
     6361 tggtatagat gatgttaaaa taggatttag cccatgtata ccttctaata taattggctg
     6421 atttgaatct atctttatct ttttaccagt gtattctctc ttaccttctt taaaattgaa
     6481 tcttatatca tcaatttctt caccagctaa aagtttcttt aagtctaagt taaacttctc
     6541 taaatctatt gcataaattg attcaaaatc ataatttcca tattcatcta aaggagtatg
     6601 ttttctatct ataaaataat catctaaaga aattgaaata ggatttaaat tatttactct
     6661 aagatgtatt gataatctgt gagcaaaact tgtttttcca gatgaagatg gagctgctat
     6721 taaaactact ctcttattgc catcctttat catatccgct atttgggata acttcttttc
     6781 atgcaatgct tcaaatgtcc ttattatatc tccatattca ttgttttcta ttattttatt
     6841 aagagatact accttgtcaa ttcccataag tcttgaccat tcttctgctt cataatatac
     6901 acttgatagc ttaggttgag gtacaaattt cataggaaga gtcttatctt cttcactagg
     6961 tcctaaaatt ataacgcctc cattataaag cttaatatca aatgatttta tataaccagt
     7021 tgatggcaac atataaccat aaaaatggtc tatatagttt ctacacttat atactttagc
     7081 actatcatac tctttgtatt tcaaaagctc tgccttttca tacatttcat tattttcaaa
     7141 gatagaaata gcttcatttc ttgtaacttc aattttttct ataacataat cgttgtttac
     7201 tatctctttc atcttgtttt ttatattttc aacatctgct tctttaagct ttctatctat
     7261 atgaacttca caatatagcc catctgatag tgaatgttct attgtaactc tgctatccca
     7321 aaagatttct ctacatgcca ttacaaatat aaatgacatg actctaaagt aaactctctc
     7381 tccatcttca ttagtagtat ctaaaaaatt tatctcacaa tccttcttaa ctctacaatt
     7441 tagctctttt agtttattgt ctacaactgc aagtgttata taacccttat gctcatcaca
     7501 aaattctttt gctatatctc ccaatctgat tccaggggaa ttttcttcta atgaatattc
     7561 cttatctttt ccttctattt ttaagtttat ttttttcact tcaacatctc cttccttttg
     7621 atgaatataa attttaatta atgaggcata tcaacaagat aaagagaggt atttaaccct
     7681 ctcctacaat attattcaat ttacttatag tatcttcaat gtcttcgtaa tttatttcta
     7741 atactaaata ttctgcatct aatgttaaga cctctttaat cccctcttca gataaaattc
     7801 ctcttcctat accaacatgt gtatcattga taccatcatt atcacttaag tgtatcagtc
     7861 ttattttatc ctttaagttc tttacataag catcctcatc aattaaacag tgtccagtat
     7921 cgtaacaaaa acaaatctta gaatttttaa tcctcttaaa tatatattca aaatcataag
     7981 atatatttcc aatatttgaa aaatccccac cttttgaata agtattctct atacatacat
     8041 atgaatttgt atttaattta tctaaaaaat ccacactatt tcctaaatat ttgtctctat
     8101 tctttctaac tctatttgtc ataacatatc ccaaatgaag gttaaaatac cttaaatcaa
     8161 atccttttag ttcaaattct atcttctcaa taaaatttat ccatgaattt cgtatatact
     8221 ctatgttttc gcaagtattt aattccatag gcaaatgtat ccctatagat aatcctaact
     8281 tagaaattct ctctttatat ttacataact cactacattc agatatattg tcaattccaa
     8341 tctctaaatg agttatttgc tttatactct cacaaatgtt taaagcctcc tcaatattaa
     8401 acaataagga cgctatacca actgccatac aaatcaccta ctcaaaaata cctatatctg
     8461 taaaaggaaa tactcttaaa ataacttttc ctttaatatt ttcttcatct actaatccca
     8521 cttctttata tctactgtct aaactaactt ctctattgtc tcccatcgca aatacttttc
     8581 ccttaggaat caccatatca atatcgcctt cagtataatt atcatgtata tatgaaactt
     8641 catttaataa cttaccattt atataaacct tagaatcctt aatttttaga tggtcaccag
     8701 gaacacctat aacccttttt acaagctctt ttttccttcc attctcttgt aataaatcag
     8761 tcttaaatat cattatatct ccacattttg gttctgataa cttatatgtc atcctgtttg
     8821 ctaccaaata atcatgtgat ttaagtgttg ggtacattga ttctccattt actaatgttg
     8881 gttttataaa acgagttata ataaatgcaa aaaaaagtgc agtaataatt atttttatcc
     8941 actctacaat ctcttcttta atagtttcat tcattcacaa attctccttt atagttatat
     9001 tatctttatt gataatgtct cttaaacact atcttttaga taagtctact aaataaggtg
     9061 ttgtcttttt tatatagaca actattgtgt atggtctggt gatttcagct aatcagcgat
     9121 tgctccaaat tttatatgtt tagtcaatag ttcctatctt agaaaatgga tacaatctga
     9181 ctagaacctt tcctaaaatg gtatgctcgt cgaccaatcc aacctcatcg tatctactat
     9241 ctaaactttt ttctctatta tctcccattg caaataattt tccttctgga actacgatat
     9301 caatatctcc atcagtacga ttattatgta tataggaagt ctcatctaac aacttatcat
     9361 ttacatatac cttggagtct tgtattttta catggtcacc aggaaccccg ataactcttt
     9421 ttactaaatc ttttttcttt ccattttcct ctgttaagtc ggttttgaat actattatat
     9481 ctttgtattt tggctctcct accttgtacg caattctgtt aactatcaaa taatcacgtt
     9541 caactaatgt aggatacatt gattctcctt tgactattgt tggcactata aaacgagtta
     9601 ttgcaaatgc caaaacaaga gctatgacaa tcacttttat ccattctaca acttcttttt
     9661 taactgcttc acccactaaa atcgtctcct ctgtgttaaa tattaagtca atctacagca
     9721 aaataatcta ccataaacct acaatataaa tattatactt cttttttcta atagtttaaa
     9781 atacttttct aagaccttta ggataatgat tcttaattac accagaagat tcttttcccc
     9841 aacctaaagg tattccttca accgaaacca aaacccatcc tctactatca ttagaattta
     9901 caacatcccc ttttaaatat ttaagaattg attcactctg tacactgaag ttttcaacat
     9961 tttttacatc ttcaactttt aagtaatgag atagtgcatg agaaggctca aatctattct
    10021 tctttaaaac ccctaaatgc aaaccatatc tcaatacttt tagttttttc gtatctggat
    10081 atatttcagg aagtaggtac aaattttctc ctcttaaata aaatttattt tttttattac
    10141 tataaaaatc aacctttaaa aatttctttt caaactccct ataatccttt atttctttat
    10201 ctaaattttt tatttttaat tctttgacat tacattcttc ttcttctaat ttttttattt
    10261 tggcaacaaa atgaccttct ccctttatct tatgaggcca taatctttcc atttcaataa
    10321 gttctgcatg tggatattca ctgataaact catttataac atgctcattt tcctcccttg
    10381 aaaaagtaca agttgagtaa attaaaatgc catccttctt taacatttta tatccatctc
    10441 ttataatctc tttttggatg gattgacatt ctactacctt tgcataactc caatctgata
    10501 tggcaacctc atccttccta aacatacctt gcccagaaca aggtgcatct ataataacct
    10561 tatcaaaata cccattaaac gtctttctta agctattaga atcggtgttt gttataatac
    10621 aatttctcgc tccaaatcgc tctaaatttt cccctagagc cttaattcta ctagcattaa
    10681 tttcattaga aaccaataaa cctgtatcgt ttagcttaga tagtatatat gtagattttc
    10741 caccaggagc agcacacata tcgagaactt tttcaccctc tttaatatct gcctttggta
    10801 ttacactcat tgcagaaggc tcttgcaaat agtatgctcc agcctcatgc agtggatttc
    10861 taccaggtct gtcaatttta tcatcataat aaaaaccttc ttctgcccat ggtatttgat
    10921 tcaaatcaaa caactccaaa tcaaataatt ttttcttgtc aatttttaaa gtatttattc
    10981 taagcccagt agtcttactt tcctcataac tttttataaa atcatcatat tcatctttta
    11041 gtatttcttc aatatcttct aaaaattcct ttggtaattc tgtcaaatct ataatccccc
    11101 ttcaatctgc tttacttgtg tgtcatttat tatactttaa ttatttatgt ctttccttta
    11161 aattttattt cttcttcttt ttcctaagat taccgaaaaa cactttttta gagtgattca
    11221 tatatttctc aagtttatca agatttatct tccacaaaat tattacataa actataagta
    11281 atataatagc ccatctatat tctatcatca atatgaaatc aaaaactcca tgtaataaaa
    11341 ttggaattaa cactgccata aacaaatact cacgtctttt atttttattt ccctcaaatt
    11401 tatacttaga aatatagtag cccatagtaa ttgcaaacat tacatgagct ggtatagaga
    11461 taactgccct attaatacca acttgcagtg ctagatttct actttcagaa aaaatataaa
    11521 ccatattttc aattgttgca aatcctaaag ctaaaaatac agagtaaata ataccatcta
    11581 acttttcagt aaagtgtttt tctttaatta gggcaggaat cagtatcaga gcttttacac
    11641 cttcttctac aagtcctgca actacaaatg atacatatat tagttctgaa tacccttcaa
    11701 atacattaaa cttcatcagt aaattttcta aaagtataat tataacactt agaaaagcac
    11761 ctattaaaaa aaatttaatt agatggatta ggggttctct acaatatcta tctttagacc
    11821 ttatccaaaa catccctatc aatataggaa ctattgctaa taaaaataaa tctagcttca
    11881 tagattattt tcctttcaaa atttttgatt tatttatgat ttatatcatc tacttatttt
    11941 taacttattc gtgttttaaa tacatactaa aatatacatt aaattatttt aaattattta
    12001 aatacaaata tactcaaata ctcaaaaaaa tttaaattta tatctatatt atttaacaaa
    12061 tttcaagcta tattcatatc ttgaaatata agggaactaa attcatttaa agaaagggtg
    12121 atttgatgca agatggtttc ttaacagtaa gcataattga tgctactaac aatagaccta
    12181 ttcaaaatgc agtagtaaac atatattcaa tgtctaatgg tagccaaagc tcatcaactt
    12241 tataccaaaa cctaagaagt aatgaatctg gtcaagtgac tggtttagta ttaccagcac
    12301 cagatgtaga ttactctcta caaccatctg atgttagacc ttatagccag tatatagtgg
    12361 aagccatagc agatggatat gagacagttg tcatagaagg aacacagttg ttagctacaa
    12421 ttgaagcaag acaaggagtt cccatgtctc ctagaacaag aagtaaacgt tctttctcaa
    12481 gacaaagtga gttgattttt gacataggag aacatactct atatggtact tatcctccta
    12541 agattccaga aagtaactta aaaccattac cacctcctac tggtttcgtt gtattagata
    12601 accctgtagt gcctgaattt atagtagttc atgatggttt gccagaagat tcatcagctc
    12661 caaactattg gattccattt aaagagtaca ttaaaaacat agcgtcatct gaaatatatt
    12721 caacttggcc tgaacagaca atctatgcaa atgtaatagc tataatatca tttactttaa
    12781 atagagtctt tactgaatgg tatagaaata aaggatataa ctttacaata acttcaacaa
    12841 cagcatacga ccataagttt ataaataata gaaacttatt tgaaccaatt aacgtagttg
    12901 tagatgctat ttttaatact tttataaaaa gacctcctac atcaagacag ccactattag
    12961 cacaatattg tgatggacaa aaatcccaat gtcctgacca aatgactcaa tggggcagta
    13021 aagacttagg tgaccaagga tatgactatg agagtatact aagatatttc tatggagatg
    13081 aaatagtatt tgaaagagca cctatagtaa gtggtgtacc agtgtcattc cctggtacta
    13141 cattacaagt tggttcttct ggacagtatg ttagaaccat acaaaatcag ttaaatgcta
    13201 tatctaattc ttatccagct gtaccaaaag ttatagagga tggtatctat ggaacagata
    13261 cagaaaacgc tgttaagata ttccaaggaa tctttggttt acctcaaagc ggagtagtag
    13321 attttaaaac atggtatgaa atatccagag tttatgtagc tacaactaga atagcttctt
    13381 taaatccttt aatttaaagc ttgatttaga acataaactc aaattattga ataaaattat
    13441 taatttaaaa taaaaatggc tgttatgaac taatatatac cataagtatt acttataagt
    13501 attgtagtct taacagccat tttttagtta taacttgttt ttttagaatc ttaaaacttt
    13561 aaatggggga ttttaagctt cttaaaaaca gtgcttagca acttagattt ctttaaaata
    13621 ttatatttat ttttaagttt aataaaacgc tattctaacg aaaaacctac tttttattta
    13681 aaatctcact tattatttaa aacactatct atcacttaga gtattactcc ttattattta
    13741 aaacactgtc tatcacttag aatattactt cttattattt aaaacactat ccatcactta
    13801 gaatattact tcttatataa attattattt attataaata caaataatat taatttaaaa
    13861 agttattcct aatttaaata atactattat cttcatcagt gaacctaaac tgcaacttta
    13921 gacttattag ctaaagccaa tattagacct aaagctatag atgcaaatgc agaagcaatt
    13981 actctatact cagcagggaa taagaataca attgtatgac atggtatcca gaaaaatggt
    14041 atagttttta aaacactaaa accaaggtaa gcattccagt ccacaatatt taccacatct
    14101 ttaagtttta ccttaatccc ctttccaacc tcatattttt tatctataaa acagtccata
    14161 accttatgga ataaaaatac agttggtgac attagtatat tcatagttgc actcttaaat
    14221 agagcatttg caaaagctat tccctcaaat ggaagcatac catctacttg tgcttgacct
    14281 acacctgatg agtagattga cattccatat gtggtaacta cacctattat tccccaaact
    14341 attgctctat aaaatactcc tactggtatt ttccaatcct tatacaaaat tctagcacca
    14401 agtaaatctc ccattgtagc taatacagca aactttaaaa aacctccaat atatggatgt
    14461 gcacctgttg cttccataaa tacttctcta ctacttggta ttgctaagac agcaataata
    14521 caaataagaa ctccactcca tataaaatcg ccttttttca tatttataaa tactatttaa
    14581 tatataaata gttctccttt ctattaaaga tttatttaat ttacaatcta tctacaatta
    14641 tttattaatc acttattatt agatattcat tttccatatt taaatttatt ctatcccttt
    14701 atctatttat tttcttacta ctctatccac cattatctat atatttattt tacgattact
    14761 ctttcaccct aaccctttaa ttatctattt ttttctgata ttcttatcta ccatcatcca
    14821 tatatttatt ttacgattac tctttcaccc taacccttta attatctatt ttttctgata
    14881 ttcttatcta ccattatcta tatatttatt ttacgattac tctttcaccc taacccttta
    14941 actatctatt ttttcttact attcttatcc acatcatcca tatatttatt ttacgattac
    15001 tctttcactc taacccttta actatctatt ttttctgata ttcttatcca ccatcatcca
    15061 tatatttatt ttacaattac tcttttaccc taacccttta actatctatt tttttctggt
    15121 attcttatct actattatct gtatatttat tttatgatta ctcttttaac ctacccccta
    15181 tactttatag taatatgttt ttaacctttt tcattattcc caatatgctt tataactaca
    15241 aatactctcc ccccttaaaa ataataataa aattaatcaa taaatttata atcatattat
    15301 tactctatgt attaagcaat tactatgcca ataaactttg atttttttaa acaaatctta
    15361 aaaatcctaa aatgctaatt ttctttaact tttaatgaat ctaatagata acatttaatt
    15421 ttcgttatat ttttcagaat atttctatta ttatttttaa gaaaaaaaaa taatgttaac
    15481 atttcttaac atacattaaa aaatgttaac attataaatt gggtttaata cttttacttt
    15541 gactcgattt acttttattc tacttcttac tttattttta acttagactt tattttttta
    15601 ttacttattt gagtcttatt tattttatta tcactccata tgttcaatta catttgtcta
    15661 aattctttga tacttttatt acataatttt aacaagctta agtgttattt gggtagtgca
    15721 gttgataatt atttaaataa tcatcaactg catgtttata tcattacagt agcttaatgt
    15781 atttaatcat acttacttag gattttgaaa tatcttagag attatattta acattagtca
    15841 attgctgcat aattgtaagc aattgaaatt tgagacccat cttcattttc tatagtgata
    15901 ggcttcataa tttttaatcc ttcagttttt atcttctcta attcttgttc cattttctta
    15961 attgtttcct caaggtcttt ttgacttaca tctttgactg cattagggtc tttagcatag
    16021 tcttttaatt cttgaatatg tttagcatac tcttcataag tataatgctc aacaccatac
    16081 ttttccaatt cagtagggtc agttattcct gattttatta gagattcaag tttaggtaaa
    16141 gcaatattag atgaatcatt tccatttgca aatactgctc cagttcctcc tagtaccatt
    16201 gcagatacca tcattgcact tataagggat ttttttattt tcataaccaa tcttcctttc
    16261 ttagagattt atttgggtag tgtagtttga aatgactatt gcaatagtta tcaaccacat
    16321 ccttagacta tcacagtagt catgtttatg tcaagccttt ttaaaaaact tttcaataaa
    16381 aaatactatc tcaaaacaaa attaattatt ttgagatagt attttaattc taaatctttt
    16441 atattactaa gatttatggt tactctatcc atgttctata ttgttcttta ttaactgcta
    16501 acaaattccc ttttcttgat atgacaaagc gattatcatt atagccactt cgctcaattg
    16561 aaacttctcc tgaatcagta taaagtacat ccttactatt actagattta tttacatacg
    16621 tttcttcact attctcagaa ttagttactt tgctcataga tgattcttca tgctctctaa
    16681 attcaattac agatactttt gccgtactaa aatgagtctc tccatcactt ttagctttac
    16741 taacaagaat atcagattct actttgggag aatataaatc agttgattta ctacttattg
    16801 aaaatccaat tttatcactt tgttcatttt ttgttaaacc aaaagattca acctcattaa
    16861 tctctttatt ttctgttgca aaaacaagac ctgtcccaca aactaataca gttgaaacta
    16921 aaaaagtaac ataagttgat tttttaaatt tcataatatt aactattctc ttttctattt
    16981 ttgtctttcc aaatccacta catattgtca ttgaaaatgg attattctcc tctgctagac
    17041 ttattagtgt ggttgcataa tcagccctat attcttcacc aagtatactc ataacctttt
    17101 catcacaact aaattctatg tctttattta gtaaaaaaaa catcacccaa actaagggat
    17161 taaaccaatg gacaacaaga gctacaagca taaataactt taacagatta tcaaagctat
    17221 taatatgaac catttcatga gttaaaacaa agtctaaagt ttgattatta gcataattca
    17281 tttgctttgg caaaataatt cgtggtttta aaataccaga tgtaagtggt gtcctagtaa
    17341 attcagatat gcaaacaata aatcttctaa aacggctatt cttacacatc cattttttaa
    17401 tgaactcatg attttttata tgaattgaag ttttaaaaag attaattatt ttaaaatatc
    17461 taaagcataa aagtatgcta aacataatag taactataaa agacaaaatg cccaacattt
    17521 ccaacatcat aatattttca atattagaca ttatttcaaa ttcgtcagaa ttgtttttac
    17581 ctgtagagtc actagctttc ttaagagtat aattttctgt tgatgtatta cccacttcca
    17641 catcaaaagt cgattcaatt atactataaa tactaaaacc agaatttaaa cttactggta
    17701 ttagaagttt aaataaaaca atcaaccaca ataatacaaa taccttttta ggtaattttt
    17761 taaataaaca tgctcttata aaaataacta ttaaaagtaa tactccagaa gtaaaactca
    17821 tttctaacaa tttaatgaac ataaatctta cctcacttta atttttcaac aatttgttta
    17881 agttttgcaa tctcatcact tgaaatattt tctttattaa taaaagctga aagaaatttt
    17941 tctgaagacc ctccaaacat tttattaatt aaagtttctg tttcctgttt ttgtacttcc
    18001 tctttgctta ttaaagcttt acaaacaaaa ttcggctcaa ttctctctat tgcacctttt
    18061 gacactaatt tttttataac ggtatacgta gtgtttctat tccatcctgt ttcttctttt
    18121 aaaattttta ctaactctcc agccttgctt tctcctctct cccatagaac ttccattact
    18181 ttaagttctg aatcaaataa ttttatctcc atataaaatc gctcctcaaa tatagttatc
    18241 acaatagtca ttactataat aaaaaactat catagtagtc tatactatgt caatcattta
    18301 ttttttaata ataaaaactg tctttttaaa aaattcttat aaaagacagt tttatcaatg
    18361 ttatacttta atgtttaatt aaagccatat tttatttaaa tatacaacta tctatcttag
    18421 tacatagtat aagatttttc tttatctaat agtatatcct ctacatctgg gttttctttt
    18481 actaattgtc tataaaaatt acaattagta acacccacat atggaattag ccatagtaga
    18541 cctattccaa gagtagctat tgaaagtata taccatccta taaaactaaa gtttagtaca
    18601 aataatttaa gtacatttcc tttcatcata tctaaactag cacccattga tttaaataca
    18661 cccatattat ccttgtcttc acaaattaag aaagtagctt gagaaacata taatagcaat
    18721 atataaaccg gtaacataag aacaagtatt ataagaacta gagttattcc agtagaaata
    18781 atagtcccta ttccaacatt atcacttgat aaatattcaa ttgctccact agtaagtaaa
    18841 actccaaatg ataaaaacgc tcctataatt gtgattataa aagaataaat ataaacaata
    18901 attgattcaa ataaataaaa accaaaactt cttaaataaa ttctcccact tacaaagata
    18961 tctcctatct tgactttttc atctttagta attttcaaga acatacatgt aaatcctgct
    19021 gaaacgcaaa aagctattac agccactaca atagtagtca atggttcaaa aggtaatcct
    19081 acaattgata ttaatactat caatgcaata tatactgcca tagaaattgc acatatcccc
    19141 cagtttccag ataactgagc tttagatact cgttttaatt cttgccttga taccataaaa
    19201 aacacccctt tcattttata aaaaaacttt taaaaatata aataatcttt taacgtataa
    19261 tattaatata tcatattttc gactatataa gatacaatta ttcaccatgt tttatcaaga
    19321 tatgccataa ttgtattact agttttatat agtcaa
//
LOCUS       DAFKMX010000064        19192 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFKMX010000064 DAFKMX010000000
VERSION     DAFKMX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.064"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..304
                     /locus_tag="LZB08_001807"
     CDS             <1..304
                     /locus_tag="LZB08_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315311.1"
                     /translation="MFISYSSFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVIS
                     IVGDAVGSILPNEDTNMVYHLYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            526..1776
                     /locus_tag="LZB08_001808"
     CDS             526..1776
                     /locus_tag="LZB08_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315312.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVGYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            1967..2851
                     /locus_tag="LZB08_001809"
     CDS             1967..2851
                     /locus_tag="LZB08_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBM7315313.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            3213..3830
                     /locus_tag="LZB08_001810"
     CDS             3213..3830
                     /locus_tag="LZB08_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBM7315314.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIIKPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            3938..6469
                     /locus_tag="LZB08_001811"
     CDS             3938..6469
                     /locus_tag="LZB08_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBM7315315.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKKALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            6903..7604
                     /locus_tag="LZB08_001812"
     CDS             6903..7604
                     /locus_tag="LZB08_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBM7315316.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            7654..8736
                     /locus_tag="LZB08_001813"
     CDS             7654..8736
                     /locus_tag="LZB08_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7315317.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINYSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            8997..10097
                     /gene="vanG"
                     /locus_tag="LZB08_001814"
     CDS             8997..10097
                     /gene="vanG"
                     /locus_tag="LZB08_001814"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBM7315318.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            10094..10900
                     /locus_tag="LZB08_001815"
     CDS             10094..10900
                     /locus_tag="LZB08_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBM7315319.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            10918..13056
                     /gene="vanT"
                     /locus_tag="LZB08_001816"
     CDS             10918..13056
                     /gene="vanT"
                     /locus_tag="LZB08_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBM7315320.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            13370..13990
                     /locus_tag="LZB08_001817"
     CDS             13370..13990
                     /locus_tag="LZB08_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBM7315321.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(14156..14692)
                     /locus_tag="LZB08_001818"
     CDS             complement(14156..14692)
                     /locus_tag="LZB08_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBM7315322.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(14805..15479)
                     /locus_tag="LZB08_001819"
     CDS             complement(14805..15479)
                     /locus_tag="LZB08_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBM7315323.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            15682..17556
                     /gene="asnB"
                     /locus_tag="LZB08_001820"
     CDS             15682..17556
                     /gene="asnB"
                     /locus_tag="LZB08_001820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBM7315324.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(17648..19084)
                     /locus_tag="LZB08_001821"
     CDS             complement(17648..19084)
                     /locus_tag="LZB08_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBM7315325.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLSNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKISPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         7244 a         2041 c         3237 g         6670 t
ORIGIN      
        1 aatgtttatt tcatattcat catttatttt gttattatat acatcaatct caatgggaca
       61 attgccaaaa ttcaataaac atagaaacat agtggcattt gcttctttta tagctataaa
      121 tatagtaata tcaattgttg gagatgcagt tggaagcatc ttaccaaatg aagatacaaa
      181 tatggtatat catctttatc agagtcctag ttttatgctt gcaatacttg gtagtcttgt
      241 agttgcaata gcgttatttt tcgctacaaa atttatatta gataaaaaat taaatttaga
      301 ataaatgttt ttatatggag ctatcttttg agtaatgtta aaagatggct cttattattt
      361 atgtagttaa ttcatgtata taatatgaat agtgttttat ttctagttaa atatatacat
      421 atgttgtata atgatattgc gtgtaaaaat ttgtaagaat aactatacac tttatataag
      481 ttatatcaag aaaatttaaa aatatatgga tttgagggag aagatatgaa aagttataca
      541 aaggctaagt ggagtgtttg gggtataatt acattttcat ttgttttagt attattttta
      601 aggatgtcaa ctgctgttgt ttcagacaat ttagcaaatg agttaggttt taattctata
      661 caaatttcaa atatagcatc cttttgccta tatgcatatg catttatgca aatacctgca
      721 ggaattttga ttgataaata tggagcaaga aaaattagca gtcttggaat aataatggca
      781 agtttagggt caattttatt tggtttgatt caaagtatag aattagcata tatttctcgt
      841 gtaattgtag gggcaggaac atcagtaata ttactttgta ttttaaaaat tcaagggaga
      901 tggtttaata agtcagaatt tgcttctgca acagctaaat tttcatttgt aggaaattta
      961 ggtggagttc ttgctacttt tccacttgtg tttttgtctg aacttgttgg ttggagaaat
     1021 tcatttttat taatcggtat aattggtgtt gtaattggat gttttatgta tattattgtt
     1081 agagatacac caaaagagta tggattcaat gtagatactg aaccctatga aaattcagag
     1141 aaggttaata ttgttgatgg tattaagtct gttataaaga acaaatcaac atggtataat
     1201 tcaatgataa tgttctcttt tgtgggtctt acaagtgcat ttataagctt gtggggagta
     1261 agatacataa tggacgttta tggtgtgagt aagagttttt ctgcgtttat agtatcattt
     1321 tttacatatg gatttatatt tggttcaata attatggatt ttgtatttgc taaaataagg
     1381 tctagcaaat ttaatataat aaaatttgga gcaatgatag atttgtttat atggattgta
     1441 attgtagttg gataccaggt aaaaccacca attatagttt taccaatatc attctttatt
     1501 atgggatgta tagttatgtc acatctacag gtttttaatg atgccaagta taaaaataaa
     1561 gaaatttatt ctggactggc aactagtgtt attaatactt ttgaatttat aggaagtgga
     1621 attattaatt tgattatagc tatctcttta caagtaaatt catataatat agttgatgga
     1681 tataaaaaag gttttgtagt atttatagta ctaagtatta taactatagt atcatctcat
     1741 ataggggtaa aaaatgatga ttttaaggct atatagtgtt ttattaatag tttaatttag
     1801 tattatattt tgcatatata taacaaatag tttgcatttt cctaatatgt ataattggaa
     1861 tataaatctt gtttactatg tacaaatggt attgtattat attaaataga aaataagaac
     1921 tttaaactat aatattggtt ctaaaatata aggtaaggag ttttttatgg atacgacgaa
     1981 aatagatgat ataagcaagt tagatttaaa taatttagat attaataaga tggatatggg
     2041 aggtataatg gaaagtctat tagaatgggc tactacaagc ggagtaaaac ttataatagg
     2101 catacttata ctttctattg gatttaaaat aataaaaaaa tttgtaaacc atgttatgtt
     2161 attacttagt aaaagagata ttgatttaac tttaagaaag tttttaaagt ctcttctttt
     2221 gagtgtactt aaagttgccg ttataatcat tgttctggaa tattggggaa tgagtttgtc
     2281 cagttttgct gctgtgatag catcagcagg ggtagcaata ggtttggctt tacaaggtag
     2341 tctatcaaat tttgctggag gatttattat actattgatt agacctttta aagtaggaga
     2401 ttacgtagaa gcagcaggtc atggaggaac ggttgaacaa ataggtcttt tttatacaca
     2461 attggttact ccagataata aacagatact tatacctaat ggttctgtat caaatgatag
     2521 tctaataaac tattcagcaa agaatactag acgagttgat ttgactttta gtgtagggta
     2581 tgaagatgat atactacatg ttagaagagt acttaaagat attgttaata atcataaatt
     2641 aataataaat gaaccagaac catttatagg agtagtagaa catggagata atgctattaa
     2701 atttgcaact agagtttggt gcaagacaga agattattgg acaatttatc ttgatttact
     2761 agaagaggtt aaagttaggt ttgacgaaga aggaattacg attccatatc ctaaaatgga
     2821 tttaacggta aaagaattaa acaaaattta attttttaat atagtgttta aatttaagaa
     2881 tatcatatag atatacttat aaggtttgta aaaaattaat aagtactgtt attttgtaag
     2941 taaaaatata aaaaaatggg agtgtctcaa aatgaacttt ttagttcatg aggcactctt
     3001 tttgtacgaa ataaaatata tttttattat ttcaacaatg aaaaagaggc ttgtttccat
     3061 tttgagacag tataattttt atgtaacttt aattttagta aatataaagt tttaaaattg
     3121 ataaaatagg aggaattttt aatataaaag ggtaaataaa gggtttagag ggcataatat
     3181 agaataaatt aatatattta aggagggttt ttatgtgtaa aaaaacgtat ttattagtgg
     3241 taagtatgct tatagcgggt ttattaactg catgtagtgg gagtccaact aaggattcag
     3301 gacaagcaca agataataca aaaaaggagg caaatgcatc aaataacgct ttaaaagatg
     3361 aaaaaaataa tgaaaattta atggaacaag attttaaagt gccatacaca gatgcaataa
     3421 acatatttaa agataaatat aaggatgctg atatagtaga tttaagtctt gagagagatt
     3481 taaataaatt tgtttatact gtggaaggtg tagatgataa taatgaatat aagatgaaaa
     3541 ttgatgctaa tacaaaagat gtattagaag ataaaacaga aaagttggat tcagaagatt
     3601 taaatggagt tgctagaaaa gaaaaattag atttaaatga cataattaaa cctcaacaag
     3661 ctatggaaat tgcattaaaa gaacaaaatg gaatggttaa agaatggagt ttagacaaag
     3721 atttagatgt tactttttat aaaataagaa tagataaaga taaaaatgaa tatgacataa
     3781 aagtagattc taaaaaggga actgtattga aggtagaaaa agaagattag aagatttaaa
     3841 ctatctttaa aataatttct tttttgaata aattttgttt aaaatataac aagcagggta
     3901 taaagagact aaataactat tttaggagga gtacggtatg tcattgaaat cactaaaaat
     3961 aaaagaaaat ttatattggg taggttcact tgaccctgat ttaagagtat ttgacataat
     4021 tatgtataca ccttatggga ctacttataa ttcatatgta ttaaaaggaa cagaaaaaac
     4081 agttttattt gagacagtta aagataagca ttttgacaat tatattgaaa gattaaatga
     4141 tttaaatata gattttgaaa aaatagacta tattgttgta agtcatactg aaccagacca
     4201 tgcaggaagc gttgaaaagc ttttggattt agctaaaaat gcaaaagtag tagcttcaga
     4261 aacagcaatt aaatatctta aggaaatcgt aaacaaagac tttgaatatg ttgcagttac
     4321 agatggagat acattatcaa ttggggacaa aactttagaa tttttctctg tacctatgct
     4381 tcattggcca gataccatat acacatatat aaaagaggat aaaactcttg taacatgtga
     4441 ttcatttggt agtcactata gtaatgataa gatagtaaat acacttgatg aaaatgaaga
     4501 aaaggattac ttggatgctt taagatacta ttatgattgt ataatgggtc catttaaacc
     4561 atctatggta actgctatag aaaagataaa agatttagat atagatactg tatgtccagg
     4621 tcatggtcca gtattaactg aaaatcctag aaaaattata gacctttatt acaactggag
     4681 tgtaaatgaa caaataaagt tagaaaaaga agttacaatt tgttatgttt ctgcacatgg
     4741 atatacaaag attatggcag aagctataaa agcatatatt gaaaaaaata gcaactataa
     4801 agtaaattta tttgatgtaa tagagcataa gcaagaagat attttggcaa aaatagcagt
     4861 atcacaaggt gtattatttg gaacaccaac aatattagga gatgcgttaa agcctatatg
     4921 ggatatatta atatcgttaa acccagttct tcatggtgga aaagttgctt cagtatttgg
     4981 ttcatatgga tggagtggag aaggtataga aaatgctatg gagagaataa gtcagcttag
     5041 aatgacagca gtaaaacctt ttgctgttaa ctttaaacca tcaaatgaag agattgataa
     5101 attacattct tatacaggta aattcctaga taagttaaat tctacttttg gaagtaaaaa
     5161 gaaaactaag aaatttaaat gtgtaatttg taatgaagtt tttgaaggtg atagtgctcc
     5221 tagtgtatgc cctgtatgtg gagctaagga agaccaattt atagaagttg aagaagatga
     5281 agttactttt agaaaagata ctgatgaata tttcgtaata gtaggaaatg gagcagctgg
     5341 tttttatgca gcagatgcta taagaaaaag aaataaaact tgtaagataa ctatgatttc
     5401 taatgaagat gagttgactt actatagacc tgcactttct gatggtataa atgaagaact
     5461 tggttcagat ttctacatgg aagataaaga ttggtatgat aaaaataata tagtagtaat
     5521 attaggaacg aatgtagata aattagatga agtgaataaa actattatag tgaatgatgg
     5581 agctattaaa tttgataagc ttgtaatagc tacaggaagt agaaacttta taccaccaat
     5641 taagggccat gatttagaaa atgtgtttac tcttagaaat ataaaagatt tatatagtgt
     5701 aaaaaaagca ctagaaaaat ctaagaaagt tgtagtgatt ggtggcggtt tattgggact
     5761 tgaagctgca tgggaattta gactaaaagg attagaagta gtagtagttg aagccatgga
     5821 tagcatacta tcaaaacaac ttgataaaga aggtagtaaa atattagaac aatgtgtaag
     5881 agatacagga atagatgtaa gattgggtgt agcagttgat ggtattgaag gtgatggaaa
     5941 agctcaaaaa gttgtattta aagatggaga tagtgtcgac tgtgatatgg tagtattttc
     6001 aattggtgta agagcaaaca ctcaaatggt tcaggataca tctgtaaaaa ttgacagagg
     6061 tattgttgta gataagacat tacagacaaa tattaaagat atatatgctt gtggagatgt
     6121 tgcacaagtg ggaaatatta gtttagcaat atggccatct tcagtagaaa tgggaaaaat
     6181 agcaggggca aatgcaagtg gagataattt gacatttgaa agtgaagtat atccagtttc
     6241 attagatgca atgaatgtaa aagtatttag tattggtaat atacaaaatt ttgataaaga
     6301 aatttcatca aaagatgaag gtcaaagaat atataaaaaa ttatttatga aagatggttc
     6361 attagttgga gctatactta taaatgattt aagctgtact gttaaattga taagattaat
     6421 atctgaaaaa ggtgattttg aggatattat gaaggctgat atactataat ttaaatacat
     6481 taaaataaaa aggagttgtc ttaaaattga tttttttcta atcaaatttt aaattcaact
     6541 ccttttttga atttttatat aaaataacat ggaaatttta gtaatgtctg tggttacata
     6601 cataggttta attgtttttt atatggattg tctctggtat aataaattga attgtgtttg
     6661 actgatagat atttttattg aaagttaata cctgttatgc gataataagg acgtgttagg
     6721 ggagttttta taataacaat taatctcagt ttttgtgttc caatggtagc atcaacacat
     6781 tctgatgtag tagttctttt cttataaaaa ttagttacaa atatgcttgt aattatttga
     6841 tatgtaaatt taccaatatt ataaatgatg acctcgataa taaggagagt gaaaaataat
     6901 ctatgaacga aaatattttg attgtggatg atgaaaaaga aatagcagat ttaattgaag
     6961 tatatttaaa aaatgaaggt tttacagtat acaagtttta tacaggtaag gaagctctag
     7021 aatgtattga atctaaaaat ttggatatgg ctattttaga tattatgctt cctgatattg
     7081 atggatttca tatttgccag aaaattcgag aaaaatacta ttatcctatc attatgttaa
     7141 cagctaaaat tgaagatata gacaagataa tgggtttaac cataggtgct gatgattata
     7201 taacaaagcc atttaaccct ttggaagttg ttgcaagagt aaagacacaa ttaagaagat
     7261 atatgcgata taataattct tatgaacaac agtcgataat tgttaatgag tatgatatta
     7321 aaggattgat tatcaataaa gaaacacata aatgtagttt atttgggaaa gaagtagcat
     7381 taacaccgat tgaattttct attctttggt atttatgtga acatcaagga aaagtggttc
     7441 cttctgaaga attgtttgaa gctgtatggg gagaaaaata tcttgataat aataacacag
     7501 ttatggctca tatagggcga ttacgtgaaa aaattcatga accttctaaa aacccgaaat
     7561 ttataaaaac tgtatgggga gtaggttata caattgaaaa gtaaaaatat taaagtcaat
     7621 cctgacttta ctcaagtgag aaataaaatt tttatgagaa cagctttaat ggcatttata
     7681 gccattattt tcatatggat tttatattct tttatattgt atggtaagtt ttctatctgg
     7741 atagtatcat tctttgaaaa tgcttttcgt atggaatata aagctgcatt gaatttatat
     7801 cagcagattt tccgtaatag catggagctc ttcatgatac tagctatggt gattgtgttt
     7861 tttataatat tccgtgttta tttaaattgg tttacaaaat attttagtga aataaatcaa
     7921 ggtattgact ctcttatcaa agaagatgtt ggagaagtgg ctttatctcc agagttattg
     7981 gcaatagaaa agaaaataaa ctcaattaaa catatacttg aacaacgtaa atttgagaca
     8041 caaatggcag aacaacgtaa gaatgaactt attgtttatc ttgcacatga tttaaaaaca
     8101 ccacttacat cagttatagg gtatctgaca ctacttcgag atgaaagtca gatttctgaa
     8161 gaactgcgaa aaaaatattt atctatttcg ttggataagg cagagcgtct tgaagattta
     8221 attaatgaat tctttgaaat tacaaggttt aatttatcaa atatatcact tgaatacagt
     8281 acagtaagct taactcgtat gcttgaacaa ctaacatatg agtttaaacc tactttgatg
     8341 agtaagaatt taaaatatac actgaatgtt acaccaaata tgatgataag gtgtgatgtt
     8401 aataaaatgc aacgtgtatt tgataactta ttacgaaatg ctgtttatta tagttttgaa
     8461 gatactacta ttgaaataac agctgtacag gaggatgatt atataaagct taaatttata
     8521 aattatagca atacgattcc agaggaaaaa cttgagcgta tttttgaaca attttacagg
     8581 ttggatacat ctcgtaattc aaacagtggt agagctgggt tgggacttgc tatagctaaa
     8641 gaaatagtgg agcttcataa cgggacaata acagctcata gcgagaacaa tataattacc
     8701 tttaaggtta ttattccgat tgtaggaaat ttgtaagaat ttaagtataa aaaaatagga
     8761 aattctttag gataaataaa taaagagcac tcttgttatt gatataatta ctgtatcaaa
     8821 acaagagtgc ttttattttg atacagtaat tatatcaata gaaaaatgtg aaaatatatt
     8881 atgtggaaca gatgcaattg taagtgaagg ttgattggat ttgaaataga aaaagtattt
     8941 taacatgttt ctgtagattg tataaaaata aataaatcaa caaaggagaa tcaaagatga
     9001 ataataaaaa aattgcagtt atatttggtg gaaactcaac agaatatgat gtatcattac
     9061 aatcagcttt tgcagtactt gagaatttga atacggaaaa atatgatatt ataccaattg
     9121 gaattacaag ggagggtaat tggtatcatt atattggaga ttataataat atccagaata
     9181 acacttggtt tgaaagggat actgatataa ttccagttat tgtttcgcag aaccgtgtca
     9241 aaaatggtat tattgaattt ttaattcctg atgctgtttc tcagaatcat attccaaata
     9301 gtgctattga atcatgtgaa aatgatatta aatacactaa acttgatttt gtatttcctg
     9361 tattgcatgg aaaaaatgga gaagatggaa cagtgcaggg gttatttgaa ttggcaggta
     9421 tacaggttat tggatgtggt actctttctt cagcattatg tatggacaaa gacaaatcac
     9481 ataaacttgt tcattcagaa ggtattaaag taccaaaatc aatattacta aaatactcag
     9541 ataaatttaa tgtggctgaa ctgtcaaaag acttaactta tccattattt ataaaacctg
     9601 tgcgtgcagg ttcatcattt ggtattagta aaatttataa aaaagaagag ttacaagtag
     9661 cagtagactt ggcttttgaa catgataatg aagtaataat tgaggaaaat attgatggtt
     9721 ttgaggtagg gtgtgcagtt ttaggaaaca atgacttact tgttggaaga gtagatgaaa
     9781 tagaattgtc aaaaggattt tttgattata cagaaaaata tacattgaaa agttcaaaaa
     9841 ttcatatgcc agctagaatt gatttggatt gtgagaaaag aatacaagag acagcaaaaa
     9901 ttatttatag aactcttggg tgttctggat ttgctagagt agatatgttt ttaactccat
     9961 ctggtgaaat tatttttaat gaagtcaata caattcctgg ctttacttct catagtcgtt
    10021 atccaaatat gatgaaaggt attggattgt cttttgaaga aatattggac aagataattg
    10081 aggtgtacat aaaatgatga ataatatttt attagataaa aataaagaag ataaaatgac
    10141 aaatactatc ttattgacta aagataaagt ttatgatggg aatctaattc tagtaaatgc
    10201 ttttttacca gttaaaacat ctgaagatat agatttaatt cctgtagata caaggtttcc
    10261 aagtattctt atgaaacgtg aagctacaaa cattttacaa aatattttaa aaagtatatg
    10321 tggaataaat gaaatagtac ctgtaagtgg atatcgtact gcagaggaac aacaagatat
    10381 atattcaagt tctctgcgtg acaatggaaa agactttact aaaaagtttg tagcacttcc
    10441 aaatcacagt gaacatcaga ctgggcttgc tattgattta gggttaaaga aagaagttat
    10501 agattttatc tgccctgaat ttccttataa tggtatttgt aatgagtttc gtaaggcagc
    10561 actacattat ggttttatag agcgttatca gtgtaaaaaa gaagaaataa caggaatatc
    10621 tcaagaacca tggcattttc gttatgtggg atatccgcat tctgagatta tcaataaaat
    10681 gaatctcgct cttgaagaat atatagaata tattaagaga ttttcgtata caaattgttt
    10741 aaaaatagaa gataaagaaa aaattataga aatcttttat gtgcctattc atactaaaga
    10801 agaagtgaga atctctatac ctgaacaatg tgtatatcaa atatcgggta acaataagga
    10861 tggttttatt gtgacatttt ggagaaatag aaaggactaa gaaatggagg aacaagaatg
    10921 attaggaata aatcttatgc agggcttgat tattttagga ttatagctgc attacttata
    10981 attgcaattc atacatctcc attattatca tatagtaaaa tggctgactt tgtgttaact
    11041 agtataattg ctcgtattgc tgtgccattc ttttttatgc tatcaggctt ttttctaatt
    11101 tcaacatata attataattg taataaactg aaatcttttc taaaaaatgt attatttatt
    11161 tacgggatag ctattgtaat ttatattccc ttaaatattt acactggata tttttccatg
    11221 aataatttag tgcccaatat tattaaggac attgtatttg atgggacttt atatcattta
    11281 tggtatctac ctgcatcaat tattggtgca agtattgcat actttcttgt aaaaaaacta
    11341 ggtttaaagt actcattagt aatcactatt gttatttatg taattggtat gtttggtgat
    11401 agttattatg gttttttaga aaaaatacca ctattaaaga atttatatgg gtatattttt
    11461 gaagtatcag attatacacg aaatggtata ttttttgcac ctgtattttt catacttggt
    11521 ggaataatat ctaataaatc taattatatg tcaactaaaa atagcttgat tggatttctt
    11581 atatcattct tacttatgtt atgtgaggga atgtttttac ataaactagg ggtacagcgt
    11641 catgatagca tgtatattat gttattgcca tgtatgtatt tcttatttac agccttaaca
    11701 tttttgaaag gttctcgagc taggttattg cgaacatcat cattaattat ttatattata
    11761 catcctatga tgattgttgt tattcgtatg ttttctaaaa tattcggaat acaaactgtc
    11821 ttggttgaca atagccttat tcattatata gtggtaagta tagcatcttt tatagcttct
    11881 ataattatta tattgattct taaacctcta aaaaaagact tgaaaggaga acataaggta
    11941 aataccaata gagcttggat tgaaattgat ttaaataatt tggaacataa tgtaaagatg
    12001 atacaaaaag ctatgccaga agattgtgaa ttgatggcag ttgtaaaagc taatgcctat
    12061 ggtcatagtg cttttgaagt agcgacttgt gcaaaccaaa taggtgtaaa agcatttgca
    12121 gtagcaacaa tagatgaggg gattgattta cgtcgatatg gaatactagg agaaatattg
    12181 attcttggat atactaaccc aataagggca aaggaacttc ataaatacaa tcttacacag
    12241 agtgttattg accttaatca tgctatctct ttaaacaaac agaaatacaa cataaaatca
    12301 cacattaaaa ttgatacagg tatgcacaga ctgggttttg atgttaatga tgtgaggtca
    12361 attttaaagg catttaaatt aaaatactta gatatatgtg gcatatttac acacttgtgt
    12421 gtttcagata gtctgaaaga tgaagatgta gactttacaa ataaacaaat tgaatgtttt
    12481 tataagttaa ttgattttct gttaaaaaaa ggtataaaaa ttcctaaagt acatattcaa
    12541 agtagttatg gtttttttaa ttatcccaat cttaagtgta attatgtaag aattggaatt
    12601 gcattatacg ggatattaag ttctccaaat gatacgacta aattgcaact tgatttacga
    12661 cctgttttat ccttaaaatc acaagtaatt cttattagaa aaatacaaaa agatgatagt
    12721 tttggatatg gaagagtctt tattgcaagc agagatagcg taattgctat attatctatt
    12781 ggctatgccg atggtatacc tagaaattta tcctgtggaa aaagttacgt aataataaat
    12841 ggttgtagag cagcaatagt gggtcgaatt tgcatggacc aacttgctgt tgatattaca
    12901 gatataccta atgtagaagt tggtaatacg gcaataataa taggaagaga taatttatct
    12961 gaattatctg cttctgaagt tgcaaataac tcatgtagta tatcaaatga attattgagc
    13021 agagtaggaa ggaggttaaa tgttataaag aagtaactca attaaatagt aatatttgtt
    13081 gtactgtgta atttatttta ctctgactag acatttttag taatattatg tctaatatat
    13141 attttttata acttacatga caatttaagc tataaaaagg tgatgtttca gatttttatt
    13201 ttgaaacatc accttttttg ttataatata taataaaata attatacctt aactgtaaac
    13261 ttatatagat tatttacttt ttaatattag attcattttt tgagattggt atataataaa
    13321 catatagagt atataataat agttaaagtt aaaaaagggg aaaagcaata tgcagtatat
    13381 acaagaacta cttgatttga ctcaaaatta ttgggtgtta gccacaataa tcggcttatt
    13441 aagtgcattt atagaaagct ttataccagc cttaccactt gttgctatag ttacagcaaa
    13501 tgctgcaata caaggattat ttattggatg tttgttatca tggattggct caggacttgg
    13561 gacaacttca ttattcttat taataagcag atttaatgat agtaagcttt ttaataagtt
    13621 gagaaatagt aaaactgaaa aagcaataag ttggatggat aaacagggat ttaagttatt
    13681 atttatagcg tatgcctgtc catttatgcc cggttgttta gttactatcg catcagcttt
    13741 tggtaaaaaa gatttaaaag attttgtgcc agcaatgttg gcaggaaaat ttgtaatgtt
    13801 tatagttata agttacgtgg caagtgatat agaaggtttt ataacaagtc ctttaaagat
    13861 agcatcattt atattattgg tgtttttgtc atggaagatt ggaaatagag taaataaaaa
    13921 tcttgaaaat cataattatg atttccatca taaaaaacat cacaatgata aagatgataa
    13981 aatgatttag cttgtacgaa ataaaacgta tcttaaaatg atttagtact aaattatttt
    14041 aagatacgtt ttattttttg tattaaagta caaaattttt agttcttttg taaactaaaa
    14101 tatataaaac ttatttatac ttagttcaaa atattgttat ttattttttc tacaactata
    14161 ataaaatata aaattgatat ccaatcaaaa aaataactat gaaatttaat agcttagata
    14221 tgaatttttc tgtttttgag ttagttttta taggtagtaa agaaatgttt atggtaatgg
    14281 gataaaacaa ttcaatacct tctttagtaa taaggtcaag acacaagtga gatagatatc
    14341 ctattataaa cccactggac aaacatgaga aaactatatt attgttagtg aagattgtca
    14401 gagatttaaa aaaactataa ataagtaata aggcaattag actgtgagta aaccctctat
    14461 gtctaaacct acttccaaat aatttgtata ttaacataaa ttttttactt atgtaagaac
    14521 ctctcatatc tatatctgga aataaagaac ctaaatatgc aaaataaata taaattataa
    14581 ttaatactat tttatatgca atatcgtatt taactaggta tagattgttt ataaagggga
    14641 gtatcaaaag tgcgaatata taacctcccc tttcatgtgt ttcttttatc atataaaaat
    14701 cctcctgaat cgtcactata aacattatat tggtaaattt agactatctt ttattatatt
    14761 atatagcaaa cataagtttt tttcaaaagg attttaatat ttaattaatc ttgagatttt
    14821 aaatttttgt aattttctaa agcaccattc caatttacta cattaaacca gttgtcaatg
    14881 tattcatttc ttctattttg atattttaaa tagtaagcat gctcccaaac atcaagtcct
    14941 attataggag ttaggttttt acttacaggg ctatcctgat ttggagtagt cataatagat
    15001 aatttcccat ctttagtagc tacaagccaa gcccaaccag aaccaaagac atctaaagca
    15061 gatttttgga actcttgctt aaatttttca aaagaaccaa agtctctatc aatagcttct
    15121 tttaaagatt cagaaggtat ggttttttct ggtgtcatta tatcaaaaaa gaatttatga
    15181 ttataagctc cacctgcatt atttcttaca gttgtagcaa tatctttagg taaagaatct
    15241 aaattttgca ataattcaca taaagaataa ttataaagct caggatattt ttcaagagca
    15301 gcatttaatt tatcaacata agcttgataa tgcttatcat gatgcagttt cattgtttct
    15361 ttatctatat aaggttcaag tgcatcatat gcataaggta atggtttaac cttaaatttg
    15421 ttattttcag gtgtaaaagc aaatgaagtt atgcactgtg agattataaa taaagacata
    15481 ataactggta ttaatatttt tttcttcata aaaatcacct cctaatatag tatttctaga
    15541 aataaaaaaa atatgaaata ttaacagata atttataaga atttaatagc aatttcattt
    15601 taaattgaat aagttttaaa gccaaagaca aaactatgaa atatagaata agactaaagg
    15661 acagaaagga attttgatta tatggacggc aatatttcaa tttattataa aggtgatata
    15721 tctaaaaaag aagatattaa atttaaaata aaagaatctg taaataatac aagactttct
    15781 agtagaatag agtataagga tgaggaaaaa acttttgatt ttgaaagatt taatgtaatt
    15841 gatgctattt atggaaataa accatttgaa aaagatggaa aagttattgt ttttaatgga
    15901 aatatatata actatcatga aataaaagaa gaattaatta aaaaaggaca caattttact
    15961 acaaatgatg atattgaagt cttattagca tcttatatag aatttggtaa gaattgtgta
    16021 cataagatta aaggaatgtt caactttata atctatgata gagaaaatga aagtatattt
    16081 ggagcaagag atttatttgg gataaaacca ctttattata taaataagga aaatgcaata
    16141 attttttcct cagagtataa gtttttactt gaatatatga aaaatttaaa tataaatgaa
    16201 agaagcttac aaagttattt ttcatttcaa tatgtacttc ctgaagatac tatgatacaa
    16261 ggaataaggc tgataccagc aggtcactat tttagggtag aaaatggtat actatcttta
    16321 aagagatata ataaacttga gtttagaagt agtacaaaat ttttttatac taaaaatcac
    16381 ttaggcaata gagatgtgaa cgaggatgat ataagaaata tagtagtaga ttcaatagta
    16441 actcatatgg aagaggagaa agaaattgga acatttctgt ctggtggaat agattcatct
    16501 attataacta ctgtggcttc acaaattaat ccaaatataa aatctttttc tgcaggcttt
    16561 agtgtcaaag gatatagtga attagaagta gcaaagaaaa cagctgataa gttaggtata
    16621 gaaaatatcc agattaatat aacacaagat gaatatataa aatcattacc gaatgtaatt
    16681 tattctttgg atgaccctat agcagaccct tcagaagtag gtctttattt cttaattaaa
    16741 gaggcaggta aacatgttaa agtagcattg tctggagaag gagcagatga attatttgga
    16801 ggatataata tatataaaga gtatagtact atgaaatcag ttgtcaattc acctacttat
    16861 attaaaagta tattaggaag agtttcagaa cttatgccaa acataaaagg aagaaattat
    16921 ctttatagag ctacaacacc attagaaaaa agatatatag gaaatgcaaa agtttttgaa
    16981 aacagcgaag taaaaagatt cttttttaag tataaggaaa agaatatata tgaatacctg
    17041 ttgtctaatt tgtatagaga tgcacaaaaa aataattacg attatataag taaaatgcaa
    17101 catatagatg tgaatacatg gcttcaaggc gatatacttc aaaaaattag caaactttct
    17161 acagctgagc aggttgaact tagagtacca tttttatata aagatgtatt tgatgtagca
    17221 aaaaatttaa gaatggagca aaaaataaat aagaataata caaaagtttt acttagagag
    17281 gcgtttagag aaatagtccc agaacatgtt gttcaaagga aaaaattagg atttccaacg
    17341 ccaataagag tttggttaaa agattctcta ggggatattg taaaggaaac tatttataat
    17401 tcaaatgtag atgaatttat agataaaaaa tatgcaataa aacttttaga tacgcatctt
    17461 aaaggtcata gagataattc gagaaaaata tggactatat ttacattttg tctatggcat
    17521 caattattta ttgagcataa aaatgtcgag tattaatact tttttcaata ttaatcagag
    17581 ggcaaaagca tgcaatgttt tcaccctcct gattattatg gaataggata agtagaattt
    17641 tgattgatta gtttactatc tcaataaaat tattggcaaa tattttgaca agattacttt
    17701 caacagctaa atttaagttt tgtccatcat catatccagc ggaaatcaat cctagtaagt
    17761 ttccaaacat atctaaaaga gctcctcctg aactaccact agagattgga gctgtaaatt
    17821 gtatcatttg aaccgtttca aaatcacgaa aagctgaaat aattccatct gaaactgtat
    17881 taaaaagacc taaagggctt ccaattgcaa ctattttttg acctctcaca ggttttttac
    17941 tgactttaac aggtattggt ttgcattttc tatcaacctt tatcacagct aaatcataat
    18001 ctgaatggta ttttataatt tggtaagatg tataaacttt atcatcattt tcaaatctaa
    18061 ctaaaaatga atacccatca ttgactacgt gaagattcgt aagtatataa ccttcattat
    18121 ttattactac tccagaaccg ctcttaaacg gtttattttc gttattgcac acaacaatca
    18181 taacaacaga agctgataac tttgcaaggt cttcataaga aagttctact tcctcatctt
    18241 gtacaatttt tttagatgtt agatttgtgt tattaagtgt atttttagag ttaggtttat
    18301 tcgtagtagg agaaatctta ttggtatttt cactgtaatt tatatttgta atatgccttt
    18361 caaaaggtat ttctaatata tttggagtct tcctagaaag ttcaaataga gtagaaaatt
    18421 ttgaacattc aggatataaa tcaagaatat ctttatttgg atttatatat aaaatatcac
    18481 acccaagttg tgataaaaaa tataagaaaa aaagttcttg ccttttaata tttccaaagt
    18541 atactatctt tggtgaaata gtagtcttca ccttgttata aaataattta ggtagaaaat
    18601 agtccatcca acacaaaagt ttaactgcaa agtttttgat gatagaggat gtacaatttg
    18661 gcatacttgt ggaaaataaa tctaaagtat ttttaaatgc aatcttttta gtccaattta
    18721 aggtgtcatt ttctatttta catggaaagt cgatattata taaatcccaa ctgttaaact
    18781 tagaactatc ataaatatta aaaattttaa gcatcttatc tgtttgttct ttgtcaaaat
    18841 ttatttttag attgcttaat ctaatatagg gaattgtagg attattaaaa aaataagtat
    18901 ctatattttt aattttttct atgtatttat caattgaatc atcaacacct atatatcttg
    18961 taaagtagac ttctttaata tatgaagaac gcttattttt ataggtagta tcagatataa
    19021 attcaattag aggttcagta gactttttta ttgaaatctt taatggttta atagtacaaa
    19081 tcatattttt tctcctataa taagatgata taatctatta taaaacaact taaaacaata
    19141 tagtacaata attttgtagt ttcaaaataa aatatgtact tgtttatata aa
//
LOCUS       DAFKMX010000065        19144 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFKMX010000065 DAFKMX010000000
VERSION     DAFKMX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.065"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            146..517
                     /locus_tag="LZB08_001822"
     CDS             146..517
                     /locus_tag="LZB08_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7315326.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNY"
     gene            649..1923
                     /locus_tag="LZB08_001823"
     CDS             649..1923
                     /locus_tag="LZB08_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBM7315327.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETSEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            2034..2978
                     /locus_tag="LZB08_001824"
     CDS             2034..2978
                     /locus_tag="LZB08_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315328.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3194..4531
                     /gene="rsxC"
                     /locus_tag="LZB08_001825"
     CDS             3194..4531
                     /gene="rsxC"
                     /locus_tag="LZB08_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBM7315329.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4554..5531
                     /locus_tag="LZB08_001826"
     CDS             4554..5531
                     /locus_tag="LZB08_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBM7315330.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5531..6100
                     /locus_tag="LZB08_001827"
     CDS             5531..6100
                     /locus_tag="LZB08_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBM7315331.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            6113..6706
                     /locus_tag="LZB08_001828"
     CDS             6113..6706
                     /locus_tag="LZB08_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBM7315332.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6721..7296
                     /gene="rsxA"
                     /locus_tag="LZB08_001829"
     CDS             6721..7296
                     /gene="rsxA"
                     /locus_tag="LZB08_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBM7315333.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7312..8283
                     /locus_tag="LZB08_001830"
     CDS             7312..8283
                     /locus_tag="LZB08_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBM7315334.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKVVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8503..9087
                     /gene="maf"
                     /locus_tag="LZB08_001831"
     CDS             8503..9087
                     /gene="maf"
                     /locus_tag="LZB08_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBM7315335.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9132..9791
                     /gene="radC"
                     /locus_tag="LZB08_001832"
     CDS             9132..9791
                     /gene="radC"
                     /locus_tag="LZB08_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBM7315336.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9912..10922
                     /locus_tag="LZB08_001833"
     CDS             9912..10922
                     /locus_tag="LZB08_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBM7315337.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            10932..11825
                     /gene="mreC"
                     /locus_tag="LZB08_001834"
     CDS             10932..11825
                     /gene="mreC"
                     /locus_tag="LZB08_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBM7315338.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11840..12328
                     /gene="mreD"
                     /locus_tag="LZB08_001835"
     CDS             11840..12328
                     /gene="mreD"
                     /locus_tag="LZB08_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBM7315339.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12340..15318
                     /locus_tag="LZB08_001836"
     CDS             12340..15318
                     /locus_tag="LZB08_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBM7315340.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15416..16099
                     /locus_tag="LZB08_001837"
     CDS             15416..16099
                     /locus_tag="LZB08_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBM7315341.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            16123..16920
                     /gene="minD"
                     /locus_tag="LZB08_001838"
     CDS             16123..16920
                     /gene="minD"
                     /locus_tag="LZB08_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBM7315342.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16938..17222
                     /gene="minE"
                     /locus_tag="LZB08_001839"
     CDS             16938..17222
                     /gene="minE"
                     /locus_tag="LZB08_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBM7315343.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17307..18437
                     /gene="rodA"
                     /locus_tag="LZB08_001840"
     CDS             17307..18437
                     /gene="rodA"
                     /locus_tag="LZB08_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBM7315344.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18478..18891
                     /locus_tag="LZB08_001841"
     CDS             18478..18891
                     /locus_tag="LZB08_001841"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBM7315345.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT         7334 a         2190 c         3850 g         5770 t
ORIGIN      
        1 tctttttttg acatattctt ttaaataatt taccatgatt tggtaattgg tcatcatcat
       61 tcaaattttt aacttaatat gatttaaata aatttataat aatgatataa tataaaaaaa
      121 ataaaaattg gagagtgatt tcaaaatgaa gtttaatttt tgtcataaca atttcaatgt
      181 tactaattta gaaaaaagtt tagattttta taaaaaagct ttagggctta aagaggtaaa
      241 gcgcaaggaa gcagaagatg gaagttttat attagtttat ttaggagatg gcattactag
      301 tcatacatta gaattaactt ggcttagaga ttgggataga ccatataatt taggagacaa
      361 tgaatttcat ctggctttag aagttgagga gtttgatgag gcaaaaaaac ttcataaaga
      421 tttagattgc atatgttttg aaaatgaaag tatgggaatt tattttatag cggacccaga
      481 taattattgg attgaaatac ttcctaaaaa ttactaattt taaggatatg tttataattt
      541 ctttacaatt aataagaaaa taagttatca ttaaattgta gggactaaaa atggataata
      601 atgttatgaa tttatgacta taaaaatttg ttggggggag tgatagtagt gaaaaaagct
      661 atagcagctt taggaattgg ggcagtagca gtatctgtta gctccataaa tgcaagtgct
      721 cttgaaaagg gaacagtaac agcaagtgcc ttgaatataa gaagtggacc aagttctgat
      781 tgcgataagg tagcaaaatt atacaaggga aaaactgtag agattttaga aaagtctaat
      841 ggatggtata aagtaagggt atcgagttct gttgttggat ggggaagtgc aaaatacata
      901 tcaacaagtg gttcgtctga aggtacatca agtcaaaata attcaacttc aagtgggaca
      961 actattagtg gaaatggtaa agtaaatgtg agttctagac tgaatgtaag aagtggtgca
     1021 ggtactaact attcattagt tggtaaagca aataatggag atgtagtaaa gttactagaa
     1081 caaagcaatg gatggtataa aataaaacta tcaaatggag ttactggatg ggctagtagc
     1141 caatatattt ctaaaacatc agaagatgtt ggaacgaaca attctagtaa ttcaaatagt
     1201 actaataatt ctgataaaaa accatctagc gaagaatcaa ttgaaggcaa gaatggtaag
     1261 gtaacatctg ctgtgagttt aaatgtaaga agtggtcctg ggacaagcta ttctataata
     1321 ggaaaattaa atggtggaga tgttgttgaa ttaaaagcta aaagtaatgg ttggtataag
     1381 gtaaagttat cgagtggaac aattggatgg gtgagtgcca gttatatttc tgagactagt
     1441 gaaggtacaa aagaaaaacc aaattcatca tctaaccaaa attctcaatc taatagtaac
     1501 tcaaagccat catttactgg aaattcagat aaatctacag caaaaggttc aacaatcgta
     1561 gattttgcat atactcttat tggaatacca tatcaatggg gagcttcagg gcctgataaa
     1621 tttgactgtt ctggatttac acagtatgtt tttaaacatt ctgtaggcgt ttctattcct
     1681 agagtttcta gagaacaggc taattttgga agtgcaattt ctatggggaa ttatgcacca
     1741 ggagatttag tttattttga cactgatggt gatggaacaa ctaatcatgt aggtatatat
     1801 gtaggaaata gtaaatttat ccattgtagt ggtactcaga ctaatcctaa taaggttaaa
     1861 gtggataatt taactagttc atattggtct aaagtattac ttggtgcaag aagatttgtt
     1921 tagtaagtac aatttcttat taaaaaatta gaagaaataa ttatttatat tcataaattg
     1981 tgactcttaa aaatatactt tattaataat ataattttgg gagggacaaa ctaatgaata
     2041 gaagaaaaac gatatacaga ggatttaata ataatagaca taagattgat ttacgtaaat
     2101 atgttataac gattgcttgc ttgtgtctaa ttggctatta ttcttataca aaaataaaag
     2161 atagcaagat attggagtat gtatcggcta aaataccatt tctaaacaat tcatcagata
     2221 taacttataa agatatatct gatgaattaa attcaataaa aaaagggaaa aaatctaaat
     2281 cacaaaccaa ttcagatgat aaacaagaaa ctaatccaga aaaagcggta aataacacta
     2341 aagagccaga agaagtcaag ttagctacta tagaaggctg ggatatgtat actattcaag
     2401 ttgctgcaat agataataat gatgatttga aaaaaataca gacatcatta gttaataatg
     2461 acataccttt ttcagttatg gagaaggatg gagtaaaaaa aatacaaacc tattcctctt
     2521 ttgatgaaaa tgatgttaga aagcagataa gcagtgtaag aaaggtgttt ccagatgcat
     2581 ttttatcaca cttagatgca cctatgttgt ctttagagta tacaagtaac tacgcttata
     2641 tagagagtat atctaaagaa ttaaataaat taataacaaa ctttaaagaa gaatcaagtt
     2701 tttggtcgaa tgctgaaaat aatgtagata tggaaaaata taatactatt ttgacaaata
     2761 gaaaggcaat atcacaaaat atatcaaaag aagctgaaaa gattgactat tctgaaatgc
     2821 gtttatttaa agataattta atagagtatg taaagaatgt gaatgaaaaa attgatacag
     2881 cttcaaaagc agcaaatgaa gaaaaatata gtgtaagtaa aagtctgctt ttatcatcca
     2941 tgcaggagta ctctatgttt ataaattcta taaaataaaa tacagaaaat ttggacttta
     3001 gaaagctata tgaaaaggat tgtttaatta agcaatcctt ttccttggtt tgacttaata
     3061 tagaggtatt gatttataaa ataaattata aaaatacgga tattttttta acaattgtta
     3121 agatttttta atatatatag tatacttata gaaggcagaa aaaatgtttt tttatagata
     3181 agggggtaaa acaatgaaac tcttaacttt taagggaggg atacatcctc catatagaaa
     3241 agagtactct aacacaaagg cacttgaaaa agctcaagct ccaaagatag tttacattcc
     3301 tcttcagcaa catatagggg caccagctaa acctattgtt gaagttggtg atgaagtaaa
     3361 gtttgggcaa aaaataggtg aacaacaagg ttttgtttca tgtaacgttc attcttcagt
     3421 atcaggtaaa gttattgcta ttgaacaaca tgaagtacca ggaggttcag ctcaatgtgt
     3481 agttatcgaa aatgacttta aggaagaatt acatgagagt gttcaaccaa aaggtcagtt
     3541 agaggatttg agcaaagaag atatagtagg tatcataaaa gaggcaggaa tagtaggtat
     3601 gggtggagct acattcccta accatgtaaa agtatctcct ccaccagata gcaaggcaga
     3661 agtagttata ttaaatggtg cagaatgtga accatactta actgcagacc atagattaat
     3721 ggttgaaaat ccagaagatg ttgtgtttgg tttaagagca ttaatgaagg tattagatgt
     3781 caaaaaagga tttataggaa ttgaaacgaa taaaccagat gctatagaag caattcaaaa
     3841 tgtagctaaa gactatagcg aaatcgaagt tgttggcctt caagtgaaat atccacaagg
     3901 agcagagaaa caacttattt atgcgtgtac aggcaaagaa gttccatctg gtggattacc
     3961 aatagcagca ggtgcagttg ttgacaacgt tgccacagca gcacagatag ctaagtctat
     4021 taaaacaggt atgcctttgg ttgaaagaat cactacaata acaggtagtt gcatcaaaga
     4081 gccaaagaac ctaataacaa aagttggaac attagtttca gaaataatag aacaatgtgg
     4141 tggatttaaa gaagataaga agattggtaa ggttataatg ggtggaccta tgatgggaat
     4201 agctcaatat actactgaaa tagctactaa caaaggttct tcaggaattt tatgtttgga
     4261 tgaggaagaa tcacgtactc cagatattca aaactgttta agatgtggaa gatgtacaga
     4321 tgtatgtcca tcattcttac aaccactttt cataagtgca tattctttaa aagatgacta
     4381 tgacacagct gaatatcata gagcaatgga ttgtatagaa tgtgggtctt gctcatttat
     4441 ttgtccagca agaagaccat tacttcaatc tataagatca gcaaagagag aaataggagc
     4501 aaagagaaga aagcaagctg ctcagaaata aagaaagtta agggggaaaa acgatggaaa
     4561 ataagttgat agtatcatct tctcctcatg tgagaagtaa tgaagatact tcatatataa
     4621 tgaaacaagt tattatagca ctccttccag cagcagtagc aggagtatac ttctttagac
     4681 ttaatgcttt gagtgctatg tttttttgta tacttggtac agtaggtact gaatttttat
     4741 atcaaaaatt tacgaagcaa aaaagtacta taggagattt ttcagctgtt gtaacaggat
     4801 tattattagc atttaacgta ccagcatcac ttccttggtg gatgtgtcta gtaggaggaa
     4861 tatttgcaat attagtagtt aaaatggtat ttggtggaat tggatgtaac tttgtcaatc
     4921 cagcacttgc tgcaagagca tttttattag catcattccc agtagcaatg actgcttgga
     4981 ctcaaccagg tgttaactgg ataggtaaaa atttagatgc agttactaca gcaactccat
     5041 taagcttttt gaaaaatgga gcagcaggac tggcagacct ttctagcaat ggaattagtc
     5101 ttgctgacat gatgataggg aatattggtg gatgtatagg tgagacatca gcaatattat
     5161 tattattagg tggagtatac ctaatgtata aaggcataat aaattatgtt ataccagtat
     5221 tttacatagc aactgtattt atattaacat tccttttagg tggatttaat ataactttcg
     5281 caatatatca actgtttgct ggaggactta tgttaggtgg attcttcatg cttacggatt
     5341 atacaacttc gcctatgact aaaaaaggtc aaataatata cgctgtatta gctggtctta
     5401 taacaactgt tataagaatg tatggtggat atccagaagg tgtatcttac tcaatactat
     5461 tagtgaactg tcttgctcca cttatagata agtttgttag aaatagagta tttggggagg
     5521 tggctaaata atgaacagta tggtgagatt aggtggtact ttacttgcta taagtgcaat
     5581 agctgcatta gctcttggag ctactaatca ggttacagct ccagtaatag aacaaagaaa
     5641 tatacaagca aataatgaac ttagaaaagc agtccttcca gaagcaaaag aatttaaaga
     5701 aatgaaggaa gatacttaca aaggccttgg agatgatctt atagctgaag tgtatgaagg
     5761 tttagatggt tctgaagtgg ttggatatac tttaaaagct aaaccaagtg gatatggtgg
     5821 agaaattgaa gttatggtag gtatatcatc tgaaggtcaa gttacaggtg ttgacattgg
     5881 taatatgtca gaaacagcag gtcttggagc aaaagctaag gatgatgcat ttaagggtca
     5941 atacaaagga aaaactgctg agcctcttga agtagcaaaa ggaagtacaa cagcagataa
     6001 tcaaatatta gcaatatcag gtgcaactat tacatctaca gcagttacta caggagtaaa
     6061 tgctgctata gatgttttca atagtgctct taataaataa ggagggttaa ttatgaattt
     6121 agctaaagta tttaaaaatg ggctaataga tgagaaccca acatttgttc aagttatagg
     6181 tatgtgtcca acactagccg ttacaacttc agcaataaat ggaattggta tgggtctttc
     6241 aacagcagca gttttaatat gtgcaaattt agttatatct ttaataagaa aaattacacc
     6301 agataagata agaataccta tatttatagt agttatagct acatttgtta caatagtagg
     6361 tatgctttta aaagcatatg ttcctgctct tgataaggct cttggtatat atataccatt
     6421 aatagttgtt aactgtttaa tattagctcg tgcagaaagt tttgctttca aaacaggagc
     6481 tatgccttca atagttgatg gtgtaggtca aggtcttgga tttactgttg cacttacaat
     6541 aataggtgca gtaagagaat tacttggaaa tggtagttta tttggaatga cattatttgg
     6601 agcatctttc caaccagtat taatatttat attaccacca ggtgcattct taacattagg
     6661 attcttattt gcaggattta ataaattaag aagtaaaaaa gcatagtgga ggtgtaataa
     6721 atgaatttaa tacttttatt tttaagtatc gttcttgtta ataacgttat aacatctcaa
     6781 ttcttgggta tatgtccttt cttgggagtt tctaagaagg ttgatacagc agtagggatg
     6841 ggagttgcag ttacgttcgt tttaactcta gcttcaatta taacttattt tatacagatt
     6901 ttattagtaa aaactggaac tggattctta caaacaatag cttttatatt ggttatagct
     6961 tctatagttc agttcgttga aatggtaatt caaaagatga gtccatcttt atatcaagca
     7021 ttaggagttt atttacctct tataactaca aactgtgcag ttcttggtat agctctagtt
     7081 aatgttcaaa aaagttataa tttagtagaa acaataataa acggatttgg agccggagct
     7141 ggatttactt tagcaatagt aatattcgca ggtataagag aaagactaga attagctgat
     7201 ataccagaag catttaaagg tttccctata acgcttatat cagcaggttt aatgtcaata
     7261 gctttcttag gatttacagg acttataaag ctataaaaaa gaggggtgaa aatggtgata
     7321 cttacagctg tattagtttt aggaattatg ggcttaatct tcgggatagt actagatttt
     7381 gcatctaaaa agtttgctgt agaagtagac gagagagtgg aagctatact aggagtactt
     7441 ccaggagcca actgtggagg ttgtggattt ccaggttgtg gaggtcttgc aaatgcgata
     7501 gttgaaggca atgcaccagt taatggttgc cctgtaggtg gagcagatgt tggtgcaaaa
     7561 gttggagaaa taatgggaat aagtgctgaa gcaggtgaga aacaagtagc aaaagttatt
     7621 tgtaaaggaa cttgttcaag tgcaaaagat aaatatgagt atgaaggaat atcagattgt
     7681 agagcagcaa atgttttaaa ctcaggtgct aagatgtgta aatttggatg tttaggtctt
     7741 ggaacatgta aagatgcttg taaatttgat gctatttcta tagttgatgg aatagcagta
     7801 atagatgaag aaaagtgtgt aaattgtgga aaatgtaaag aagtttgtcc taagggtata
     7861 ataatcacaa aaccagaaag tcaagaagta gttgtagagt gtaatagcaa agagtttggt
     7921 aaagttgtta aggaaaaatg tactgctgga tgtattggct gtggaatgtg tgtaaaagct
     7981 tgtaaatttg atgcaataat atttgaagac aaaatagcta agatagaccc taataagtgt
     8041 gtagggtgta tgcaatgtgt agcaaaatgc cctacaaaag taatatcagg tgacataact
     8101 aagaaaaaga aagttactat tgaccaagaa ctttgtgttg gatgtacagt gtgcaaaaaa
     8161 caatgtaagt ttgatgcaat agagggagaa ttaaaagaaa aacataaggt agatgcagat
     8221 aaatgtgtag gatgccattt atgtatggaa aaatgtccaa agaaagctat taaaattttg
     8281 taatatggac aaaataagga tatggaataa tttccatatc tctaatgagt acataaaaca
     8341 gaggtatgga ataatttcca tacctctatt ttatgtatat ttattctaat ggtaataaat
     8401 ataacaatag tgtaacaaaa aaggttcgac atttctgttg aattttttac aatatagtga
     8461 tagactaaat aggtacaaat tacgtacaaa tgaggtgaat aaatgaatat aatcttggca
     8521 tcagcctctc caagaaggaa agaaattcta gagaatacga atgtaagatt tgatataatt
     8581 aaaaatgaaa ttgatgagat tatattggaa ggtgaaacac caaaacattt agttatgaga
     8641 ttagcttttg aaaaaagtat gtctgtagca tctgaacata atgaagatgt tgttattgga
     8701 gctgatacag ttgttgtttt agacaatgct attttaggaa aaccaaaaga tgagtcttgt
     8761 gcaagagata tgttaaaaag actatctgga agagagcatc aagtcataac aggtattagc
     8821 ttaataaact tgtgcgaaga taaaaaggta attgactatg taatcagcaa tgtgaaattt
     8881 aaaacgttat cagagcagga tattgaggat tatctgaaaa ctaatgagtc atttgacaaa
     8941 gcaggagcct atggtataca gggttatgga gctttgttag ttgaagaaat tagaggagat
     9001 tattttaata tagttggtct tccaatttca aggttaggcg acctattaaa aaaatatttt
     9061 agtataaatc ttttttatgg agtgtgattt atctgaaaaa gtcatttaat tcaaccatta
     9121 aggtaaaaga aatggcacca gaggaaagac ccagagaaaa gatgcttgcc aaaggcgtaa
     9181 aaagcttatc aaatgctgag ttgttggcta tacttttaag aactggcaac aagaataaaa
     9241 atgctattga attagccaac tatataataa atagagatat tcagggtatt agacatttag
     9301 aagatatgac aatagaggag ctgtgtaata tagatggaat tgggttatca aaatcaaccc
     9361 aaattaaagc agctttagaa ctaggctcta gagtagctag ttttaaacct ataaagtata
     9421 aaataaggaa tccttgggat atacagaggt attatatgga tagcctaagg tatttaaaaa
     9481 aagaggtttt taaagcggtt cttttaaaca caaaaaatga gataatatct gatgtagatg
     9541 tatctatagg gactttaagc tcgtcattag tccatccaag agaggttttt aaagaagcta
     9601 taagaagaag tgcaagtaag ataatagtga tgcataatca cccttcagga agtgtagaac
     9661 catcaagaga agataaaaat atcacatcaa gacttattaa atgtggagaa ataattggaa
     9721 tagaaataat agaccatata attattggag atggattata ctttagcttt aaagaaaata
     9781 tgataatttg actttatatg tgataaaata ataggagaat gttttaaaca tgattcttaa
     9841 tagtaggaag gagtaaaatc atggctaagg aaaaaaagaa agaaaaaaaa ggttttttta
     9901 gttttaataa gatgacaaag gatatgggaa tagatttagg gactgcaaat acattagttt
     9961 acataaaagg acaaggtatt gttgttagag aaccatcagt tgtcgcaata agagatgata
    10021 gtaaagaggt attagcagta ggtgaagaag ccaaaaaaat gataggtaga acaccaggaa
    10081 atatagtagc tataagacct atgaaagatg gagtaatagc tgattttgac ataactcaat
    10141 caatgattag ctattttata caaaaagcgg ctgataaaaa aggtgttgta agtccaagaa
    10201 tagctatatg tgttcctttt ggagttacag aagtagaaaa gagagctata gaagaggctg
    10261 caagacaagc aggagctaaa gatgcattcc ttatagaaga gccaatggca gcggctatag
    10321 gggctggatt aaaagttgaa gaaccagaag gtaatatggt agttgatatt ggtggaggta
    10381 cttcagaaat agctgtaata tctttaggag gaatcgttac tgctaaatct ataagaatag
    10441 gtggagacga atttgacgag tctatagtgg cttatgtaaa aaaagaatat aatcttatga
    10501 taggtgaaag aactgctgaa aatgtaaaaa ttaacatagg ttctacattt aaagatgatg
    10561 aagaaataaa tatgcaaata agaggtagag atttaatctc aggattacct aaaacaatag
    10621 aaatatgctc tacagaagtt agagaagcat taaaagaacc tgtaagttca atagtagatg
    10681 caataaaatc tacactagaa agaactccac cagaattagc ctcagatata atggaaaacg
    10741 gaataatgct aacaggagga ggcgctttgc ttagaggtct tgataagctt attacacagg
    10801 aaactggtat gtctgtacaa atagcagaaa cacctcttga ttgtgttgca cttggaacag
    10861 gtaaatctgt tgaagaccaa gaaatctttg aaaaagtatt aatgatgaat actaagaatt
    10921 agaagttggt gatggccttg agatttgaca agaacaaaaa tgagaaaaaa aggattaatg
    10981 ttaaagtgat agcaacgggc gtagttgcta tcactttaat tggaattgtg ggtatatcaa
    11041 ttggtaaatt ttctagtggt agtccagtaa atttaggagt agcttcagat gctattacaa
    11101 gtgtaggtaa gggaataaat gatggatttt cttttatcaa aaatggtttt aaagatgtag
    11161 caaattttaa agataattct aaaaaggtaa aaaaattaga agaagaaaat gaaaaactta
    11221 aaaaaaatat gatagcttta aatgccaaac tagataaaac agaatcgcta gaagagttaa
    11281 aaaagacact gaactttgta caagaagagt ataaggcgac aagtatttca gcaagtgttg
    11341 ttggtaaaaa tgatggaaac tggtatgaga gttttgtcat aggtgcagga aaaaattcag
    11401 gtgtaaaaaa agaaagtata gttatgaatg gtagtggatt agttggcata gtttatgagg
    11461 tttccaacaa ttatagtaag gcaatttcac tattggattc taaggcatct gtaagcttta
    11521 aactagcaaa ggatgccaat gctaaaggta caattactca aaatactact ttagataata
    11581 aagatagtta taatagtaaa ggatatttgc aaggatatat gtttgactca tcatataatg
    11641 taattcaagg agatataata acaacatcag gtcttggatt cttcccagat ggtataccaa
    11701 ttggcgaagt tgaaaaagtg gttgatgata aagataagtc attaaaatat gtagttgtaa
    11761 aaccttatgt tgattttaag aatataaatg acgttgtagt catagaacca agaaatatag
    11821 gataaagggg ataaaaacaa tgaaaaaagt tttactttgt ctgttgggga ttgtgcttat
    11881 gactatagaa aatagtataa caaattatat agatattttt ggcataagct ttaatttagt
    11941 tcttatatat gtaaccataa tctctcttta tttagatgaa ttagaaggtg ggattatagc
    12001 agcaataata gggcttatta aagacataac cgttggtgga atttttggtg taaatgcatt
    12061 aattttgttt attattgcat atgcaattgg atacatgaga gatcagctat ataaaggtag
    12121 ttatattatt actttcgttt taatttttat aggcagttta attgattcaa ttgtcaatat
    12181 aggtacatct agtataattt atcaaagcta caacatatca acactttttg taaaaggtct
    12241 actagtagca ccaatagtga atagtttaat tggtttagta atatatcgct tatctaaaag
    12301 agcagtttta aaacttaaag aagattagtt ggtgatttca tggaagaaaa taaacaagtt
    12361 gatagactaa aaataattaa gatagtcatg ggcgtgatat ttttagctat tttagtaaaa
    12421 ataatctata tgactacatt taaatatgaa tattacaatg agttagctga gaataaaaca
    12481 tataaaaagt tggcaataga agctccaaga ggagaaataa aagatagata tggaagactg
    12541 ttggcaggaa ataaaaactt atttactgtt caagtttcag gtaacgatat taataagaaa
    12601 gatgcaaata agcattcaag agcaaatgaa atatcattaa aacttataaa tttgctggag
    12661 agaaatggtg aagaatatgt agatgagttt cctatatatg tagaaaatgg aaagtactat
    12721 tatacatatg acagagatat tagggaatat aaatcagaaa atggtattcc aaatgattat
    12781 aatgctaagg aaagttttta ttacttggtg gataaactta tatcagctgg aatattatct
    12841 caagaagata aaagattgga tgcgacaaga ttacaggcaa aactaaatga aaatggatat
    12901 tatccaccta ttttagttag taaatggatg ttcacagcag aaagagataa gagagattgg
    12961 ttggctagtt ataagataaa agaaactaaa ctgagtgcca aagaagcgtt tgaaaaagta
    13021 agaaatagtg atgcattaga aattgataaa agtttgagtg atgaagatgc aagaaaaata
    13081 atggtcgtaa gggatttgat aaaatcaaaa ggatattctc aatataatcc agtaactatt
    13141 gctaaggatg taggagaaac tacgattgct caaattgaag aaagtgctat ggaccttgtt
    13201 ggtgtttcaa tagcagtaga accagtaaga tattatccta atgggtcatt agcttctcat
    13261 atgttaggat atgtaggaaa aatgccatcc actcaaatag aatcatacct tcaaaaaggt
    13321 tatgaaactg gagacatggt aggtcttgca ggagtagaaa agagtaatga aagtagatta
    13381 agaggaactg atggttataa aatggttaaa gttgatgcat tgggtagaat cagtaaagaa
    13441 attgaatcta aaaaacctaa atcaggagat actgtgtatc ttacactaga taaagattta
    13501 caagaagttt cagataatgc attaaaacag ataatcgaag ttgcttctaa aggaggtact
    13561 tttaaaagta aatttgggga caaaccaatt agtgcatatg caggaaaagc acagtcagca
    13621 gctcttatag ctattgatgt taaaaatgga gaagtattag cttcatcaag ttatcctaat
    13681 tatgacccaa ataaatttgc aaaaggtatt tctacagagg attataaagc acttcaacca
    13741 aaaaatccaa atgacttact agcaggaagt ccacttttaa acttggttac acaaggggaa
    13801 tttcagccag gttcttcatt taagatggta acttcaatgg cagcgttaga aaatggtctg
    13861 gatccaaact ttactataaa tgaccctggg gttataatgt taggtaaaaa atcttttggt
    13921 gactatgtat ggaaccatgg tagaggaaac catggtatga caaacttata taaagcaata
    13981 caagagtctt gtaatatata tatggcaaca attggtacag gtaaaacatg gccagatgga
    14041 aaaagcattg gtatagatat gaatgcaaat aaaatacttg agtatgcaaa attatttggg
    14101 cttgaccaaa atacagggct tcaagatgaa gtagaagaaa gagcaggtaa agttcctagc
    14161 acagaagata aattgaaatc aacacaagct cttctaaagt caaatttaga aagagaaatg
    14221 gcaaatgatt ttgttgatat aactagagaa aaaaatccta aagaatatga gaaaagaatc
    14281 aatgagattg taagctgggc agcagaaaag aaaactcctg gtagagttga aacaatgaat
    14341 aggctaaaaa aattggatgt taaagaagat agaattgaag aagtagcaga tttagcagta
    14401 ttcagttatt ttaactttgc aaaatggagt actgcagata cattcaactt agctatagga
    14461 caaggtgaga atgcctatac accagctcaa atatcaagat atgtagctgc tatagcaaat
    14521 ggaggtaatt tagtagaatt atctgtagtt gatagagctg tttcaagtga ttattcatca
    14581 gttaaaatta atgaccagaa gaaagtcgaa aaaatacctt ttaaaaatcc agataactta
    14641 aaagaattaa caaaaggtat gaaattggtt gcaagacaag gtacagcgaa gagtgctttt
    14701 gctgattttc caatagatgt agcagcaaaa actggtacag ctgagaaaag tgggaaaata
    14761 cctacagata acgagtatga atatttaaaa agtcatatgt catcttataa tgtcaaccta
    14821 aatgatgcga ttaaactagc tgataagatg aaagcagaaa aagaaaaaga gctttcatta
    14881 gctaaggaaa aggaaataaa aaagaaatta gaaaataaag atttgaaaga cgaagaaaga
    14941 aagaaattag aggaagaatt agaagatgga gtaaaagtta gattagagga cactgataag
    15001 gtgaattctt catatctaag aaaagctata aaagaattaa atccaaaaat aacagatgac
    15061 caaatagata gatttaaaca agattatggt tcttttacgt ggactgttgc ttttgctcca
    15121 gcagatgacc cagaaatagc tgttgtatgt gtaattcctc aaggtgattc aagtgtattc
    15181 tcacttttac caactagaga agttattgga acttatatgg gattagagcc aactaatagt
    15241 aaaaatgaca acaagacgaa tgatgttaac aattcttctg acgaaaatat aaatttcgag
    15301 tcacaaatta atagatagct caatctatgt ataaacttta gctttatata gaaaaaataa
    15361 aagggtttaa agcgtatttg aagaatataa atgatatatc actctggagg ttgttatgtc
    15421 tttaagagaa atttgtagtc aagaactagt agagttcaag ggtaataaaa gagggataat
    15481 agtaaatata aaaagagaag ctccatttga ggaaatacag gaaaaaataa taaacaaatt
    15541 ggaagcatat gttggctttt ttaatggggc taaaattagt aaaattaata gtgattgttt
    15601 aactgacatg gagattctag aacttaaaga aggaattact tctagatttg atgtggaatt
    15661 tgttgaagat caaaaaattg aagaaaatag taattttcca acaaaatatg tcaataccct
    15721 aagatctggt gaaaatattg aatttgaagg tgatgtagtt atattgaatg atatgaaacc
    15781 tggttcaaaa gtcctttcaa aatcaaatac agttgttatg ggagatataa atgctggtgc
    15841 taaggttgta gcaggaggta atgtttttgt aatgggtaaa atagaaggtt ttgtacatgc
    15901 aggtgcagaa ggcaatgaat ttgcatatgt tgttgctgga aatcttaatc ctaaaatatt
    15961 acaaatagca gataatattg cagaagctcc agatgatgaa gaaaattatg aaagtgaatc
    16021 agaaattagc ccagaaatag cctttgtaag taatggaaga attgtaatcg aaagctattt
    16081 gtcaaagtta gacaaataga aagaaaaata gggggtagta ttatgagcga agttatagtt
    16141 ataacatctg gtaaaggtgg ggttggaaaa actactaccg cagcaaactt aggaactgcg
    16201 ctaagcctag aaaataaaaa aacagtagtt gtggatgcag atattggact tagaaactta
    16261 gatgtagtaa tgggtcttga aaatcgaata gtttatgaca tagtagatgt agttgaagga
    16321 acttgtaggc ttaagcaagc tctaataaaa gacaaaagat ttgataatct atatttatta
    16381 ccagcagcac aaactagaga taaaaatgct gtctcagttg agcagatgat tgacctgtgt
    16441 gagaaactaa aagaatcttt cgagtatata ataatagatt gtccagcagg tattgagcaa
    16501 ggctttaaaa atgcagtagc tggggcagat agagctattg tagttactaa tccagaaata
    16561 tcagcagtaa gagatgctga tagaataata ggtctattag aagcaaatga aataaaagag
    16621 ataagattag ttataaatag aattagaaat gatatggtta agcgtggaga catgatggat
    16681 aaacaagata taatagaaat attagcaata gatttgttag gtcttgttcc tgatgatgaa
    16741 agcataatta tatcaacaaa taaaggagag ccagctatac ttgattctaa gtcacttgct
    16801 ggtcaagcat acaaaaatat cgcaaaaagg atactaaatg aagaagttcc tctacttgat
    16861 cttgaagttg aagatggatt ctttggtaga cttaaaaaaa tgtttagcat ggctaagtag
    16921 caaaggagga tacatctgtg ttagatttat ttagagtttt ttctaacgag gctaaaacta
    16981 gtaaatctgt tgctaaagag aggttaaagc tagttttagt tcatgataga gtagattgtt
    17041 caccacaact tttagagatg ataaaaacag acattttgaa agtaatagct aattatgcag
    17101 aaatagaaga tgatggtctt gaaataaaaa tgtctaaatg cagaggtgag catgatgaca
    17161 aacctgtatc tgcattagtt gctaatatac cactaaagaa tataaaagat agatgtatgt
    17221 aattagtgct taaaaggggg atatccttat tggtatatgc cccttttatt taattatatg
    17281 tttaaagata gaaagacggt gattctttga aaaaaatcaa tattaattat aagtcgacaa
    17341 ttaagttgat aaaacagtta gactggaaac ttatagttac agtattagct atctttattt
    17401 ttgggcttgt tatacttagt agtgcaacac atgctaattc aacaggttca tataatcaat
    17461 tgattaaaca aggattagct tttgtacttg gtataggaat gattatagtc atattatttt
    17521 ttgactataa ccttctaggc aggtattata aagcgcttta tataataagt cttattttgt
    17581 tagccatagt tttattacct ggaattggga cagtaaaagg tggagctagg tcgtggatta
    17641 acttaggacc acttgacttg cagacatctg aaatagtcaa attaacattt gtactaagtt
    17701 atgcaaagat acttgaatcc aaaaaggata aattaaatac gttaaaggaa gttatgcctg
    17761 ttgtagttta ttctcttcca tttatcggat tattgattgc acaaccagac ttgggaacag
    17821 gaattgtatt ttgttgtatg atatttgcaa tgctgttcac agcaggtctt agctcaaagt
    17881 tgataaaaag aggaataatc atactattag tgtcaatgcc actgatgtat ttaatgatgg
    17941 cagaccatca aaaggtaaga atagaagctt ttctaaatcc agaagatgta actctaaagg
    18001 gaaactatca agttatgcaa tctctgattg caattggttc tggaggcgta actggtaaag
    18061 gactttacaa tggaagtcaa aatcaagaag attttttacc tgtacaagat agtgatttta
    18121 tatttgctgt tgtaggtgaa gaacttggtg taataggaat ggcagtgttg ataatcttat
    18181 ttatgatatt cctattaagg cttttagcaa ttgcaagaga tgcaaaagat ttttatggaa
    18241 ccctcatagt ggttggtgtt atgggaatgt ttggttacca gataatccaa aatataggta
    18301 tgacggttgc gttaatacca gtaactggag taacattacc ttttgtaagt tatggaggaa
    18361 gttcgttatt gacatcttta gcaaacttag gattagtact aaatgtgtgt atgagaagaa
    18421 agaaaataaa tttctaaatt taaatacatc taaacataaa ttgaggagat tagatatatg
    18481 aatatagcat tagtagcaca tgaccaaatg aaaaacacta tggtcggatt ttgtataggt
    18541 tatgaatcaa ttttaaaaaa gtatggacta tatgcaactg gaactacagg aaaaagaata
    18601 atggatgaaa ctgaattaaa tataaataga ttagcatctg gtcctttagg tggagaccaa
    18661 caaataggtt ctttaatagt aacacaagaa atagatttag ttattttttt aagagaccca
    18721 ttgacatctc aggctcatga aacagatata caagcgttaa taagactttg tgatgtttat
    18781 catgttccaa tagctacaaa tctagcttca gcggaaatat ttataaaagc tcttgataga
    18841 ggagaactat catggagaga agttagaaaa agcaaaagtc aacgtattta aaaaatatta
    18901 atattacagt ctaaagaaac agatatataa gtgtttaatg ccctagtctt tctgttggaa
    18961 taaacctagt acctgtagaa atcttatgaa agatttaaat gatgaagagc taaatttgag
    19021 attaaataat taggaaatag attatagata gagtagtaga atttaactat tactctattt
    19081 taataatatg aaatagaatc aagtctatta ttatatatat ttaaacttat aggtaatact
    19141 attt
//
LOCUS       DAFKMX010000066        19082 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFKMX010000066 DAFKMX010000000
VERSION     DAFKMX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.066"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(190..2577)
                     /gene="cadA"
                     /locus_tag="LZB08_001842"
     CDS             complement(190..2577)
                     /gene="cadA"
                     /locus_tag="LZB08_001842"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBM7315346.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENTSSKTSIKKELILGGLNC
                     AHCAEEINDKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2600..2965)
                     /locus_tag="LZB08_001843"
     CDS             complement(2600..2965)
                     /locus_tag="LZB08_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315347.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3164..4012
                     /locus_tag="LZB08_001844"
     CDS             3164..4012
                     /locus_tag="LZB08_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315348.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4449..5537)
                     /locus_tag="LZB08_001845"
     CDS             complement(4449..5537)
                     /locus_tag="LZB08_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315349.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5662..6390
                     /locus_tag="LZB08_001846"
     CDS             5662..6390
                     /locus_tag="LZB08_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBM7315350.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6592..7083)
                     /locus_tag="LZB08_001847"
     CDS             complement(6592..7083)
                     /locus_tag="LZB08_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315351.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7094..7537)
                     /locus_tag="LZB08_001848"
     CDS             complement(7094..7537)
                     /locus_tag="LZB08_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315352.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7527..8366)
                     /locus_tag="LZB08_001849"
     CDS             complement(7527..8366)
                     /locus_tag="LZB08_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315353.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8585..9970)
                     /locus_tag="LZB08_001850"
     CDS             complement(8585..9970)
                     /locus_tag="LZB08_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7315354.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10143..10886)
                     /locus_tag="LZB08_001851"
     CDS             complement(10143..10886)
                     /locus_tag="LZB08_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBM7315355.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IESEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(10906..12168)
                     /locus_tag="LZB08_001852"
     CDS             complement(10906..12168)
                     /locus_tag="LZB08_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7315356.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12352..13350)
                     /locus_tag="LZB08_001853"
     CDS             complement(12352..13350)
                     /locus_tag="LZB08_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBM7315357.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13334..14845)
                     /locus_tag="LZB08_001854"
     CDS             complement(13334..14845)
                     /locus_tag="LZB08_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBM7315358.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(14899..15861)
                     /locus_tag="LZB08_001855"
     CDS             complement(14899..15861)
                     /locus_tag="LZB08_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBM7315359.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(15909..16799)
                     /gene="rbsK"
                     /locus_tag="LZB08_001856"
     CDS             complement(15909..16799)
                     /gene="rbsK"
                     /locus_tag="LZB08_001856"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBM7315360.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(16841..17854)
                     /locus_tag="LZB08_001857"
     CDS             complement(16841..17854)
                     /locus_tag="LZB08_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBM7315361.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18083..19060)
                     /gene="bioB"
                     /locus_tag="LZB08_001858"
     CDS             complement(18083..19060)
                     /gene="bioB"
                     /locus_tag="LZB08_001858"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBM7315362.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
BASE COUNT         6137 a         3510 c         1940 g         7495 t
ORIGIN      
        1 ataaaagctc tacactaaat aaatacatta aataaacata atggataata aatataaatt
       61 tatatatgca aagcgctcca ataaaaaggt accaataaag ttaaaagaaa cttattgata
      121 cctttcatta ttacatactt caatatataa ttataagttt tttaaatagt atggatttac
      181 atttatccct tatctcatag ctctcatagc atttactact gctataagtg ccacacctac
      241 atcagcaaat atagcttccc acatagtagc tactcctcca gcacctagta tcataactat
      301 aatttttact cctagagcaa atattatgtt ttgccacact atcttattag tcttgtttgc
      361 tatttcaatt gctttagata tcttgctagg ctcatctgtc ataagcacta catctgcagc
      421 ttcaatagca gcatctgaac ctaagcctcc catagctata ccaacatcag cacgagctaa
      481 tactggagca tcatttattc catcacctac aaacgctatt ttctcttttt cagttcttcc
      541 ttcatataaa tcttctaatc tatctacctt ttcattagga agtaaatttg agtaaactgt
      601 atccaattct aattcttgag caatattttt tgctactttc tcattatctc cagttaacat
      661 tacaacttct tttaccccta tttcttttaa gcttcttatg gcatttttac tatcttcttt
      721 tacttcatca gagataacta tataacctct atatacttta tcaacagcaa tatagactac
      781 tgtgcctact tctttagctg atgaataaga tatattttct tttttcataa gtttctcatt
      841 acctgctaat ataaaattgc cattatgctt tactctaatt ccataagctg ctatttcctc
      901 ataactgtct attgtatcta aatcaatttt tttattatag taacttaata tggactttgc
      961 tattggatga tttgaattta cctccgcaat agctgcatat tctaataatt cttcttctga
     1021 tataccttca ggatttaact tagttacatt gaacacacct ttagtaagtg ttcctgtttt
     1081 atcaaatact actgtatcta cacttcttaa agcttctaaa tagttacttc ctttaataag
     1141 gataccattt ttagatgcaa acccaattcc actaaagaaa ctaagtggta ttgataaaac
     1201 taatgcacaa gggcaagaaa cgactaagaa tatcaatcct ctatataacc aatcactaaa
     1261 tacctcacct gagattacaa gtggtggcac aaatgctatt aataaagcag ctattactac
     1321 tattggagta tagtatcttg aaaacttaga tataaagttt tctgtctttg attttttaat
     1381 gctagaattt tcaacaaggt caagaatctt agaaactgtt gattctccaa aactctttgt
     1441 aacttcaata ctcaataaag catttttatt tataacacct gataagattt catctccttt
     1501 ttcaacttct ctcagcactg actctcctgt gagtgcagaa gtatctaaca tagaaactcc
     1561 atctactact acaccatcta atggtacttt ttcaccaggt tttactacta ttatatcacc
     1621 tacatttacc tcttctggtg acaccacctt aacttcagaa ttaactttta gatttgcata
     1681 gtctggtctt atttgcataa gtgaagttat agactttctg gatttaccaa ctgcaacacc
     1741 ttgtaaatac tctcctactt tatagaaaag cataacccct actgcttctg ataactcccc
     1801 tattgctaat gctcctactg ttgctagagc catcaaaaag ttctcatcaa atactcttcc
     1861 ttttgatata tttcttattg ctcttaaaag cacatcacca cctactatta cataggcaac
     1921 tataaatact atattgctaa actgactttc ataacctgta gctgtttgat atataccaaa
     1981 tatatataca agtgctccaa ttataagtgg aattaattct ttcttatttg tatcatttac
     2041 agctcctgat ttttcagtag ttctcccttt tactttgtca gtagacccta cttgtatatc
     2101 aagccctggc tctgttgaat ttattatttc ttttattttg tttataacat catcctcttt
     2161 aaagttatta cttatattca ctgtaagttt tttatttaca aagtttaaat tagaagattc
     2221 aacttctttt aatttagaaa ccttgtcatt aatctcctct gcacaatgtg cacagtttaa
     2281 tcctcctaat ataagctctt ttttaattga tgttttacta gatgtatttt ctttagaact
     2341 tatttgtata tcaagacctg gctctgttga atctattata tctattatct tttctatagt
     2401 agtatcctca ttaaagcttt cctttatatt tactgtaagt tttttattta taaaatttaa
     2461 attggaagat ttaacttctt gtagtttaga aaccttatta ttaatctcct ctgcacagtg
     2521 agcacaattt aaccctccta gtataaattc ttttttaatt aaattatttg cttccatata
     2581 tattcactcc tctcatttat catttataag tctctctaat atgatttaat ccacaatcaa
     2641 atatctgact tatatgacta tcatctaatg agtaatacac tgtttttcct tctcttctaa
     2701 actttactaa tctagcctgc tttaatactc taagttgatg agatattgca gagtgagtca
     2761 tatttaataa actcgctata tcacatacac acatttcatt tgcaaataat gcatataata
     2821 tctttactct agtagtatcc ccaaatactt taaataattc tgctaaatca tataacattt
     2881 cttcatctgg catagttgat ttagcctctg ttactatttc ttcatgtact atattacaat
     2941 tacaatcatt tatctcttct ctcattttta atcctccatt attttaatat atgaataact
     3001 gttcatatgt tcattatatg ttatcatttc taaatttgca acaataattt ttattaattt
     3061 aatataaata gaatgcacat attataaact tttttaatat atatattatt agaataagtt
     3121 tttgtaaact tttttatagt tttctaggag gtgcaagttt aatatgagta acaagaaaaa
     3181 aaaggattta gatactagtt acatgcctta cttagaccca gcgaactatg cagattatat
     3241 tactaatgaa gaaattcccc aagatgaatt gagaaatgac cctcaaattt ctaatggtta
     3301 tacaaaaata cctaataacc agaacataaa taacctatat cctaataatg taaatcatta
     3361 tcctaaaagt agtaattctc caaacaactt gcctaacaca aataatacat ccggaaatat
     3421 gaattacaat acatctgcta caaataatat gcctaataac atgaataata atacgggtat
     3481 gcctaataat atgtctaaca atatgaatag taatatgggt atgcctaaca atatgtctaa
     3541 taacatgaat agtaatatgg gtatgcctaa caatatgcct aataatatga atagtaacat
     3601 gggcatgcct aacaatgttc ctaataacat gaatagtaat atgggtatgc ctaacaatat
     3661 gtctaataat atgaatagta atatgggtat gcctaacaat atgtctggca atatgaataa
     3721 caatatgtat ggtaatacag gtatgcctaa taacatgaac agtaatatgg ggtataatat
     3781 gaataatact atgggtattc ctaacttctc atgtaatcaa aatatgcctc ctaatgtact
     3841 tatgatgata ccaggagtta tatgtcataa tacaatgcaa ggtatgcctg tagttatgcc
     3901 tagtactatg cctcctaata tatatcctac tccttatggt tctagtaata tgtctataca
     3961 aggtatacca caggctacta atattgaaga atttgatgag gaagaaatgt aatatatatt
     4021 tttttaagta agttgtataa aacattaata attgaccata ataataaatg tattcctcat
     4081 aatattcccc caatattatt ataggtatag aaagttgact gaatatgcgc ttattcggtc
     4141 aacagatatt aacataatct aataagataa acatagaaaa agtcagataa aatttaattt
     4201 tatctgactt tttcatttaa aaatgctctc ttgtgttggt gggtcagaat agtatgatac
     4261 tcgtatttga gactaatatt ggcaaacaac ataaagaaaa gtataaattt tttataataa
     4321 actgtataaa attttaataa ttgaccacaa taataaatgt attcctcata atattccccc
     4381 aatattatag aaataaattt aagcgagatt aaattcagtt aatctcgctt aaataatgtc
     4441 taaatttatt attccaaaaa atcatcaaaa tctaatgaac ctgattctgc aaaatcatat
     4501 aatgcatttt gctcaaagaa aaatttctct tcaaatttaa tatagtcgct ataattagat
     4561 tgacttattt cattgatata attgcaatca ttttcgttat aagtaccaat aaaaaactta
     4621 tttcccatta agtaatatat agctttcata tcattattta ttatatatac attttctttt
     4681 acatggtcat tattaaaatt aaatctagta aagaatatac cttcattcat attacttttt
     4741 aacagaaatg gattttctct ataaaatact cctgcaaatt cttcaggtga atttacactg
     4801 taaacactta caaattcact tttagaaatc tcatcaaata cctcaaaatc atacataggt
     4861 tctttatctg tcactaatag agaattaatt ctaaaaccag tttcataaca cttacttatg
     4921 caaaaagcaa ttttacatat ataatatcca tcattatctt catataaagc ttcacttata
     4981 tatctgcctt gacctttatc acttaccacg tttttaagta atgttccatt tatattagta
     5041 atatgcatat ttgcaatctc gtcacttcca gaaaagtact ttaaactttc tctatcccat
     5101 gctataaatc tcatggtcat atagtttata aattcaactt cctcgtcaac tcttttacat
     5161 attttattgt ataatttttc tatagttaaa tctgttttat agttttttat aatatcaata
     5221 tactcacatt tatctcctgg caatagctta tcataataaa gattcttatt accaaaataa
     5281 gatactaaaa ataaagattc atccttcgtt aattcaactc tatctgaacc aaatcctccc
     5341 atcaatcttt cttcttccat ataagcttct tcttgtgttg ggttatttaa actcacataa
     5401 tccgctaatc ctaatccctc tgcatctaat aaaaaatatt gacaaaagtg attgttttca
     5461 tcatctatcc aatttattat aagacctaaa ctgcccataa gtctagaatt tgtagccttt
     5521 gcaaaattaa aattcatata gtcacatcct ttaaactcta aaatacttgt tttttaatat
     5581 ataaattatt atacatatat tttaacaact tagatacaat ttataatata atataaggaa
     5641 atgattaatt gaggtggtct tatgaaaaat atagcagctt tttttgatat agatggtact
     5701 ctttatagag actctcttat ggttgaacat tttaaaaaat taattaaata cgatataatt
     5761 gaccaaaaaa attggtataa acatgctaga gacactttta tggattggga taaaagacaa
     5821 ggtaattatg atgattatct attagaaata tgtgaccttt atgttgattc actaaaagga
     5881 ttagataaaa cctgtatcaa ctttacaagt gaccaagtaa ttaaattaaa agcagataga
     5941 gtttataaat acacacgttc aaggattgag tggcatctaa atcaaggaca catagttata
     6001 tttatatctg gtagccctgg atttcttgtt gaaaaaatgg caaaaaaata taatgtaacc
     6061 gattttctag gaagtgatta tgtctttgaa aacaatatat ttacaggcac agttattcca
     6121 atgtgggatt ctataagtaa gaataatgct ataaacgatt ttgttgtaaa gtatgattta
     6181 gatttatcaa actcgtatgc ctatggagat actaatggtg atataaatat gctaaaaaga
     6241 gttggaaatc caatagcaat aaatccaaca aaggaacttt tatctcaaat atcacttgat
     6301 gaggaaattt ctaaaaatgt tgagataata gtagaaagaa aagatattat ttattctcta
     6361 tctacagatg taaatatagt ggatatttaa tttatactat ctattttaaa aacttatatt
     6421 atatctaata tttatatcta acatataaaa gctttgacta aaaatcaaag cttttatatg
     6481 ttgcactaag aatatattaa atttagcttt atgctttaat gtatattcat atatatcatt
     6541 tacacttatc taaactacaa tttcattaaa attaaatata aaaatcaact attatttttt
     6601 aaatagcatc tttcttaatc tattattgta ataacctata aaagaactgt aaacatagtc
     6661 atatactata aaggctatct caaatgaacc tataataaat atgtttattg gaatataaac
     6721 tattgttctt aataaaaaat acaatataag tatcattata tttgcaaata ctaatttcat
     6781 aaaatactca atatatatag acctatcttt ttctataaga tattttacaa taccatatat
     6841 tccaaaggta agtgaatata caatccattg agacttactt gccatcacta taaatcctaa
     6901 aactatagaa cctatataaa aagcgattcc actcttaaag ccccattcca ttattacaat
     6961 tgatactaaa agtgatgcta accccataat aaacaatgta tttacaggaa taatgtttgt
     7021 aagagtcaac aatatcacat ttaatgctaa taaaactcct ccataagcaa cttttatact
     7081 catatttatc atcctaacaa caagttatta aatctccacc aaaacattca caacatgaat
     7141 ctaaacacca aagtttagca caattttcac aacaatcgtc acctagacaa caacagcaat
     7201 catcacaaca acagcaacaa catccatcta aatcattacg tcttcttcta ttataattgt
     7261 cagccctatt gctataatca tttctatatt gtgtataact tctaagtgca tcactatatt
     7321 cactattttc aggctccata aattttgctc tttttaagaa gtcttcacct tcttcataat
     7381 atcctatgtt cattgcagaa aaaccagtta aataatacca ctctgcacat ttgtcagtca
     7441 aagtttttaa aaaatcatat gcttttttaa attcactatt ttctatgaat gatcttgctc
     7501 tcataaaatt ttcattatta taagtattac tgtacattct ttccacctct actttctaat
     7561 atattaatat atcttaaata aacaccagaa taaattatat tttctattat tccaacattt
     7621 ttatttagat ttagattatc aattccccta gctaaaaatc ctaatgatgt tactattatt
     7681 ttttttactt tttcttttaa ctcatcattt ttatcaatat actctataaa tggattatat
     7741 cttcctttct taaaatcttc atctaaatct tcataggcat ctagtagata tatatactta
     7801 cctatattaa aacctatcat tcttaagttt ctctcatttt catctttttt atagacaaat
     7861 acttcaccca ttatatttcc aaatatacta gatacttcat ctatatttat atttttatct
     7921 ttttctaact tatttaattc atttagttgt tgttttatat actctgcttt ttcaggatac
     7981 ttttcatatg ctaattttaa tttgttttta tatatataat atgccaattt atcttttatg
     8041 cgtttatcat ccattaaatt atcttccaat ttataatacg ttaaaagaat attcatactt
     8101 gctgcatatt ctgtaatatc atttgtaatt acctttttct ttttaaatgg atttactata
     8161 cacacttcct ctgtacttat agactctggg ttatatactg ctgttaataa aacaattaaa
     8221 aatgttatat cataattcaa agaaaaccta gagatttctc catgatttct ttttaagtat
     8281 ttacatagtc cacaatagta agctttgtaa taatcatatt ctctaaatgt taaatccatt
     8341 ttgtttattt taacatatcc aaacataaat actccttttt gtactatttt ttatttagat
     8401 tatatatagt ataacacttt ttatcaagat tatataattt acagtattct aagaaactta
     8461 aaaaattcct tctaatcatt gtatcttaaa tttaaaataa ttagaaggtt gggttaaaaa
     8521 taatattaaa ttatatactt atattaactc atacaagtta gttgtgctta tatcaattta
     8581 agttttaaat aattggtata tatttaaaac tacctacagt atccttcaat tctttttttg
     8641 ctttatttat aaccaattca tcaagcatta catcatttat tgaacatgat atagatttag
     8701 atgccaataa cattgcctta aatcccatat cagaacctgc actggcacaa gattgccatg
     8761 tatgggcaga aataccaata ggccaaactg aacaagaaca ctgtgctgta ggaattatgt
     8821 aactaacatc tccaatatct aatgataatg atatacagtt attatgagta aatgtatcat
     8881 ctgtaacctc atcatgtaaa ttcatcttag taatatcctt atttccactt aagacactag
     8941 aaacactact tcttttatct atagacactg tctcagctaa tttatctgca aaatagtagt
     9001 catcttcatt atagttttgc acacctacta atttcatatt ttgtgataga atatctgtta
     9061 atattgtatt tggaatatag tcatatactc cacttataat gtcatattcc ataatagtac
     9121 cagtcattaa tgctgctcct tttgctatat caacaatcct accaaataca tctcttacat
     9181 gctccatttt aactcctctt atgtaatacc acacttctgc aaaatctgga actatatttg
     9241 gcatttttcc accattagta gttacatagt gtactctaca actatcaaat atatgttctc
     9301 tcaaataatt acaacctata ttcataagtt ctactgcatc aagtgcactt cttccgttat
     9361 aaggagcttg tgctgcatga gcactaatcc cattatatct aaatttcaca gagtaattag
     9421 caagtgttgg gattcttata ggagtattta tatcaaatgg atgccaacta aaagcacaat
     9481 caattccatc aaaaaaacct ttttttatca tcaatacttt tccagataaa tcttcttctg
     9541 ctggacatcc atagtatttt atagtccctt tcagcttttt tagtttgatt aattccttta
     9601 ctgcaattac agctcctatt gaacctgtac caagtaagtt atgtccacat ccatgacctg
     9661 caccaccatc tacaataggt tctttaatag tggatacttt ttgagacaaa ccatctaaag
     9721 catcatattc tcccaaaact gctataactg gtctaccttc tccataacta gccacaaatg
     9781 ctgtatccat accttctaat ccctctatac taaaaccatg tttttttaaa cattcttttt
     9841 gtaaatttga agcatagtat tctttatact gaagttctgg atttttccat atcttatctg
     9901 atatatctct taattctccc tgtatctcat ctatatattc catcaaaaac tttttattta
     9961 aattatccat attaattata taaacctccc tgtttctaat aacttataga ttatcagcca
    10021 aaatgactaa ttttaactga tttaaaaata ttttctacca tacaaccatt ttatacaata
    10081 aaacctcttg acttgtaagt ttcattttat caagtaagag gttttcaagt tttataaaat
    10141 ttttagcatg ttaaccaatc aagcatatta acccataatt tattatagta tttccaatca
    10201 gtaaattctt ttggacccca gtgaggtgca cagtcagaac taaagatagc agattttcct
    10261 tttccaaact cgccaacagc tacaaatggg tctcctccaa tagttgctaa aacaggacaa
    10321 ttatctctag ctactgtttt gttataccct aaaaactttg gccattctgt tggtatacct
    10381 tttaatactg gatgttcaga ttcaataact tctggaatta ttcctgctgg tttttcaact
    10441 ctatcatctt tatctatcat tgttattggt aatacatctt tgatagcagt ttctccaaat
    10501 ctagcttttg catctattcc tgtaaatgac atatatccac ctaccatgat taacgcccca
    10561 ccattattta catactcttt gattagttca agtctatctg gattactatt gcaatctata
    10621 aaagtactat tagataataa gaacgtatta gaacctatat cagataatat tatacagtca
    10681 tactcattta actcttctaa tttatacgga aaactatctg ctgctacatg tgcaggcata
    10741 tgaactgtct catatcctcc agattctatt gcttctttca accactttac agcttcttca
    10801 taatgagttg taacaaacgt atcaaatcct tttatatgag ttatattttt tacccaagat
    10861 tcccctacta ttaatacttt tttcatttat atattcctcc tacttttata agtttaatat
    10921 tagacttgca tacataagta tactatctaa atactcctta atttcaacat attcatcaat
    10981 tgaatgacat tgttctattg aacctggccc taatataagt gttggtattt cagctaaatt
    11041 tttcattact ctcgcatcat tgccagctgt tgaacctttt atctctaatt tttttcctag
    11101 agtttcttct agtattttat gtgcattcac tacaaaatca gaatccttgt ccatctcaaa
    11161 acctaaccct gattgatata tgtttatatt tggtttatta tctttaagcc ataaatctcc
    11221 attagccctt gtcattatga catttgttac atcatttaca acttgttcat aactcattat
    11281 atttggcaag aagtgtacac ataaattaaa tacacactta tctggcacag tagaacctgc
    11341 tgttccacca tttattacac ctaaatttat tgtaggtgaa ggtaatagtg gatgtttata
    11401 tatcataagc cagttatgtt ccaactcttt tatatcttga agcaacaaca tagccttctc
    11461 aattgcattt acaccctccc acttactacc acagtgaaga gacacacctt taacctcaac
    11521 ttcaaagaat acaaatccca tatgtgcaac tattaagttt tgttctgatg gttcacaaat
    11581 tatgcaacaa tctccaccaa taccattcat aactgcattt atggtaccat ttccgccacc
    11641 ttcttcatct actactgaca ttatttttac attaccaggt acatttagac cactatcttt
    11701 tataagcttt acagctaata ttgatgctat aagtccagat ttcatatcag cagttccaag
    11761 tccataaagt tttccattat cttctgtagc acgatatgga ttatatttcc acttacttat
    11821 atcacccggt ggcatagtgt ctacatgacc attaaataca attgtcttac caggtaaatc
    11881 atcactatac ttacaaatta aattatatct atcttgataa ttgtgaccta gatttccttc
    11941 tttatatata ttttttgctt ttttaattaa ttcttcgctc atttcttgtc tatctacact
    12001 aaagcctatg taatttgcaa gtttttcaat atattcctgt ccttcttttt caaatccacc
    12061 tagtattcca tgaccaacat cttcagtctt tatacttaca agttctttta aataatctat
    12121 gtattcttgt tttttactat ttaatgtatc taagagaatt tttttcatat ttcctcctaa
    12181 gatttatttt tacatttaaa atttcaagtc taatctcatc tatttaagcc atagtaaact
    12241 ttttcactta gcatattctc attcaatgta taaagatgta tgcaaattaa agtataaaaa
    12301 tatatacaaa aacttaataa tttttatagt ttataagcta aaatttaatt actatttaga
    12361 tttagctgat ttttttaatt ttatattatt tatccatata gcaaataaca ttaccaaacc
    12421 ttttataatc atttgattat atgaagatac acttaataag tttaatccat ttcctaatat
    12481 tcctattatt ccgacaccta tacatgtacc tactatagaa ccagaaccac ccatcatatt
    12541 agtaccacca agcacaacgg ctgctatagc atccaattct gctccatctc caacagttgg
    12601 tgaacctgat gccaaacgtg atgatagtat tatcccagct aatgctgtta gtagtccagc
    12661 tagtgcgtat acttttacta taactttatt tacttttact ccacataatc tagcggcctc
    12721 ttcatttcca cctactgcat gtacatgacg tccaaaatta gttcttttta atagaaaatg
    12781 tcctgctatt acaactataa tcataattat aactggtatt ggtattggtc caatatatcc
    12841 tctaccgagc atcttaaatg catctgtatt tactgatata ggtcttcctc ctgtaaatac
    12901 aaagcttgcc ccacttatta tcgttaacat ggctaaagtt acaatgaatg gaggtagttt
    12961 ttgtgttgaa ataattgcac cttttatgac tcctaatatt gtccctattg ctaatgcaag
    13021 tatcatacct acccatatag gtgcattata attcactact gttgtagccc aaaatgtacc
    13081 tactaaagca attactgctc ctacacttaa atctattcct cctgtaatta ttacaaatgt
    13141 catacctact gcaagtattg catttattga tatttgtctt ccaacattaa ttaaattatc
    13201 taaagttaaa aagttagatg aagctacact cattattata aaaattatta caagtcctac
    13261 agctgcactc ccttggtctc taaatacact tgttaatttt gtcaataatg gagaagattt
    13321 ttcttcttta tgcttagtta ttgaactcat taattttacc tccgaaagcc aattttaata
    13381 cattattttc aaccatttct tcaccttcta aaaatccact tatatgacct tctctcataa
    13441 ccattattct gtcactcatt gatattaatt ctggtatttc agaagatatc attattatac
    13501 ttacaccact ctccactaaa tctcccatta gattgtatat ctctgcctta gcatttacat
    13561 ctatacctct ggttggttca tccataatca atatatctgg ttgtaataaa agccatcttg
    13621 ctattatagc tttttgttgg tttccaccac ttaaactttg tataacttga ttcatatgtg
    13681 gtgtttttat ctttaaagct tctatgtatt tttctaaaat ctctttttcc ttttttctat
    13741 ctataattga tttaaactta gatatctttt ttaacccagc catagttgta ttttctttga
    13801 cactcatttg taaaaacaat ccagtacctt ttctatcttc tgttattaat cctagtccat
    13861 tttctattgc atctgatgga gttttaaact ttacttcttc attgtttaaa tatatttttc
    13921 cagaatcata tgttctaagt ccaaatatag cttctactat ttcacttctc ttacttccaa
    13981 ctagtccacc tattcccaat atttcacctt ttcttatatt aaagctaata tcattaaaaa
    14041 cattgttctt agttagattt tccactcgca taatttcttc ttttatttct acttttcttt
    14101 ttgggaaaaa ttggtctagc tttctatcta tcattaaatt aactaattct tcctcagacg
    14161 atacatcttt ggtatttaaa gtcgaaatcg ttttcccatc tcgcattata gttattctgt
    14221 cacaaatctc aaagagttct tccatcctat gggatatata tattatacta actccactct
    14281 cttttaaatc atttataagt ctaaaaagta tttcagtctc ttttttactt attgaagaag
    14341 taggttcatc cattattaat actttagttt ctgatgataa aactcttgct atttctacca
    14401 tttgtttttg agctatacta agctctttga ctaaactatt tggatttata tctattccca
    14461 tattatcgaa aatcttttta gcctcttcat tcattttttt gtcattggtt aagactttgc
    14521 catttatttc ttttcccata aaaatatttt gtgctactgt taattcgtca aaaactgtta
    14581 gttcttgata tatggcattt atacctatct ttttagagat atctggggat atattctcta
    14641 tttctttgcc ttcaaaaata aattttccag aagttttcga atgtgcacca gttaaaatct
    14701 ttatcattgt tgatttacca gcaccatttt cacccagtag tgcatgaact tcgccatgaa
    14761 ataactcaaa attaacatta tctaatgctc tgacaccagg aaattcttta gcaatattac
    14821 ttaacttcag aattatatta ctcataatga cactcctttt taaataggat ttctttcaaa
    14881 taatctcatt ttattttact aattcttaaa gtcttttata ttttccttat cataaagtaa
    14941 agctggtact ggtatattct tttctacctt ttcccctttt atagctttta ctactgcttc
    15001 tacagctaaa cgtcccatct ctttaggttg ttgttgaatc atagcaagaa tagttccatc
    15061 tgttattcca cttgatgctt cttcagttaa gtcaaatcct acaatcttaa catccttatt
    15121 ttttgcagat tcaagtgcag ctttagctcc tagtacacta ttttcagctg ttgcatatat
    15181 tatatctaca tctggattag attgaattaa gttttctact gtattaagag attcttctct
    15241 gtcatatcct ggttgtgaat ttaatatctt aacatttgct gaacctttta atgcatcttt
    15301 aaatccatct attctctgca tttgtatttg agattttaaa tctgtcacta ttgctatttc
    15361 tgactttcca cctaactttt catctatgta ttttttagtg tattccccta attttttacc
    15421 agcatcataa ctaactgttc ctacataact tgtaatattt tcagtattta atacagcatc
    15481 tactgtaact actggcattt tttcaaatgc tccttctact gctggtacta tagccgaatt
    15541 atcagttcca agtactatca ttccatctac attctgttgt ataaaatctt ctattgctga
    15601 tgtttgctta gctacatctt ggtctgggtc atttactata actttcacac ctaattcttt
    15661 agctttttct tttattccct catcaatatc tataaaaaat tgatgttgaa gagttattgt
    15721 agatactcca atagttaaat ctttcttgtc tgaaccacct ttattatctg atttacttgg
    15781 tccttccatt gaacaccctg ttaacattcc tattgctagt attccagcaa ttcctaaaga
    15841 taaaattttt ctcactctca taattaacct ccatttttaa tagttttaaa tttgaacaaa
    15901 ttagattttt atttattatt taaatacttt tcaacatctt ctttataggg cataccagat
    15961 tgtgcaccta gtttagttac agaaagacct gatactacta ctgcatattc tactgctgta
    16021 tataaatttt tcccttctgt tatagccact gccaatgctc cattaaatgt atctcctgct
    16081 cctgtggtat cttgaacatc aacacttata caagatacat gcttgatttc ctcaccatca
    16141 taaaatctag ctccattttt tccttctgtt attacaagtt tatttggata tttttttaat
    16201 acttcttcta tcccttcatt tgtgtcaaaa actatcttat attcatgttc atttggtgtt
    16261 aagtaagtta ctttttctat tatatcttca tttagtttta ctgctggtgc tggatttaat
    16321 aaaaccctaa tcctattttc aaaacagaaa ttcactacat aatttattgt ttttaatgga
    16381 atctctaatt gtagcaatac tatatctgca ttctttatct cttcttcatt ctttttaatt
    16441 aattctatat ctactagctc attagtccct ggtacaacaa caatactatt atcattttca
    16501 cataatgtta taacagcaac accagaactt ttgtgattat ttctggatac taagctataa
    16561 tcaactttat tcgttctaaa atttcttatt aactcctctc ctaagccatc ttcccctatg
    16621 catgatatca tttttacatt tgctcctagt ttagaagctg agatagcttg attagcccct
    16681 tttcctccag gtgatgtaaa aaaactatta cctaatactg tttctccttt ttctggtctt
    16741 ttatctacag aacataccat gtccatgttt acacttccta tgacaactat gtttcccatt
    16801 acattactcc tttacatatc tatttatgtc tctcgtgctg ttattcactt tcttttgttg
    16861 aatctcttat gaccaattct ggtttgaaaa tcaattcatc aaacgattct tttttatctc
    16921 ctcttaaaca atttatcaat atttcactgg ctttatatcc tatatcataa gacggctgtt
    16981 ttactgtggt aagagaaggg cttattagac ttgatgtttg aatatcatca aatcccacga
    17041 cacttatatc atttggtata cttaatcctc tttctttaag acctcttatg acacctattg
    17101 caatcaaatc atttgcacaa aatattgcat caaaatctat cttttctaaa ttttttataa
    17161 attcttgtcc ccattcaatc ttatattttc cttcaaatac ataagcagga ttgtagctta
    17221 aattgcactc gattagagcc tttttatatc cttctaatct atctactgct atttcatttt
    17281 gaagtggacc acttaggtat attattttct tatttccaat atctaataaa tgttttgtag
    17341 ctatataggc cccttcaaag ttatttattt taacaattcc ttttaaggtt tctttgttca
    17401 tacttatctt cttatcaatc aagacagttg gtatttttat atctttataa tttgcttctt
    17461 tccagtttgt atttgaagat gctgcaaata taattccatc tatcatcctc tctgatagtg
    17521 ttcttattcc ttcatattct tttttagcat cttcatctgt attacataaa attatattgt
    17581 agccttcttc attagcttta tcttctacac ctcttgatac atctgtaaag aatggatttc
    17641 gtatatctgg tataagaagc cctatagtat tagttttttt agttactaaa ctttgtgcta
    17701 atttatttgg tacataattt ttttccttca ttattttcaa aattttttct cttgttgcct
    17761 gacttatatt ttcatcttta ttattaatga cttttgacac agttgtttta gaaacaccag
    17821 ccaattcgcc aatttctttt atagttatac tcaagaagtt cacccccaaa atttaggaaa
    17881 ccgttttact taaccggtta cctagatatt atcacatctt ttttccattt gcaaccattt
    17941 tatataaatt ttgatatttt tccctttttt caaaatattg caacttataa ttcaatttta
    18001 atatttaaat taatctgcaa aaaaaaatta atatactttt ataattatct aaaagtatat
    18061 taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc atttttttat
    18121 cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca gaatttactc
    18181 ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca gcatacctta
    18241 tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt tccattggtt
    18301 ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg tttattggaa
    18361 atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt tcttcactct
    18421 cattcattcc aattataccg cctacacata catctagccc agctttttta gcatctttta
    18481 tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa ttatctctac
    18541 acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt ttagcttgct
    18601 cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc ctcaatcctt
    18661 catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca cttgtgacca
    18721 atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag atttcattgt
    18781 aatctaataa tgaatattct tctacaccag ttttataata agaagactga gcacaaaatt
    18841 tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa tctgcttttc
    18901 ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca aaatcatttt
    18961 tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt tctttttctc
    19021 tcaatacttt attttttaat tttattatgt atttttccat atatcctcct atttgtcaac
    19081 tt
//
LOCUS       DAFKMX010000067        19016 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFKMX010000067 DAFKMX010000000
VERSION     DAFKMX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.067"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..723
                     /locus_tag="LZB08_001859"
     CDS             <1..723
                     /locus_tag="LZB08_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315363.1"
                     /translation="EGSEVFLEDKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLINEK
                     SNVVLDSSMLSNIVSWGITFIPSDEEHNNIIIKKISLLSEEKLSFLHNIKITGNEEVQ
                     IPVLGQGISQYEVLGLESDSKIKYSLIGKKDNLSISQKGKLTLNNKSKPGQIILQVDV
                     DDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVRIL
                     FVSLVIICFGIYLYWKKCSKTK"
     gene            741..2159
                     /locus_tag="LZB08_001860"
     CDS             741..2159
                     /locus_tag="LZB08_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBM7315364.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            2174..3382
                     /locus_tag="LZB08_001861"
     CDS             2174..3382
                     /locus_tag="LZB08_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315365.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(3456..4844)
                     /locus_tag="LZB08_001862"
     CDS             complement(3456..4844)
                     /locus_tag="LZB08_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBM7315366.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            5185..6519
                     /locus_tag="LZB08_001863"
     CDS             5185..6519
                     /locus_tag="LZB08_001863"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBM7315367.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(6648..7565)
                     /locus_tag="LZB08_001864"
     CDS             complement(6648..7565)
                     /locus_tag="LZB08_001864"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBM7315368.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            7773..8657
                     /locus_tag="LZB08_001865"
     CDS             7773..8657
                     /locus_tag="LZB08_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBM7315369.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            8870..10711
                     /locus_tag="LZB08_001866"
     CDS             8870..10711
                     /locus_tag="LZB08_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBM7315370.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            11016..11723
                     /locus_tag="LZB08_001867"
     CDS             11016..11723
                     /locus_tag="LZB08_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315371.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            11717..13027
                     /locus_tag="LZB08_001868"
     CDS             11717..13027
                     /locus_tag="LZB08_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7315372.1"
                     /translation="MLKNMNLKDVLSVKDDYIMRRVIVYFVLSVLIITLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            13144..13827
                     /locus_tag="LZB08_001869"
     CDS             13144..13827
                     /locus_tag="LZB08_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315373.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            13820..16207
                     /locus_tag="LZB08_001870"
     CDS             13820..16207
                     /locus_tag="LZB08_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7315374.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMNNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            16487..17629
                     /locus_tag="LZB08_001871"
     CDS             16487..17629
                     /locus_tag="LZB08_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBM7315375.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            17648..18172
                     /locus_tag="LZB08_001872"
     CDS             17648..18172
                     /locus_tag="LZB08_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBM7315376.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            18272..>19016
                     /locus_tag="LZB08_001873"
     CDS             18272..>19016
                     /locus_tag="LZB08_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315377.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDFMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGVSFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLSPTQSVEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKF"
BASE COUNT         7547 a         1961 c         3236 g         6272 t
ORIGIN      
        1 gaaggtagtg aagtattttt agaagataaa aatataatat attcagataa aataaaagaa
       61 ggatgcatag aagtaccagg tgaatttgaa ggcaaaattt atgtgaactt taatagcctt
      121 ataaatgaga aaagtaatgt tgtgctagac tcaagtatgc tttcaaacat tgttagttgg
      181 ggaattacat ttattccatc tgatgaagaa cataataata ttattataaa aaagatatct
      241 ttattgtctg aagagaaatt aagtttttta cataatatta aaataactgg caatgaagaa
      301 gtacaaatac cagtattggg tcaaggtata agtcagtatg aggtattagg tcttgaaagt
      361 gattctaaaa ttaagtattc tcttatagga aaaaaagata atttaagtat ttctcaaaaa
      421 ggtaaattaa cattaaataa taaatccaaa cctggacaaa ttattttaca agttgacgtt
      481 gatgataagt ttaaaattgg aaagaaaata acattaactg agtcttggag tgttaataaa
      541 aaagataagg atggtgttcc atatacatta gtatatcctg agcaatcacc aacagtacaa
      601 gatatgaaaa aaataaactt tatgaataac atcataacgt ttgttcgaat tttatttgta
      661 tctctagtaa taatatgctt tggaatatat ttatattgga aaaaatgttc aaaaactaag
      721 taaagagagg aggttagatt gtgttatttt caagtttaat ttttttattt tattttttac
      781 ctataacttt ggttttatat tatgtattta ggtttaaccg aacgatacag aacatgatat
      841 tgcttgctgt tagtttattt ttttatgctt ggggagagcc aaaatttgtt gttatcatga
      901 tagtttccat aataatgaac tatatatttg gtttactggt agatagatat agggaaagta
      961 aaataaaagt aaaagttttt ttagttttga tgtgtgcata caatataggt gttttattta
     1021 tatttaaata tctagctttt gcgctaagaa atattagtga acttattagt actgaattga
     1081 ctatacctag tattgtttta cccataggaa tttcattttt tacatttcag ggaatgtctt
     1141 atgttataga tgtatatcgt agacatggag aagtccaaaa gaaccctttt tatgtgggat
     1201 tgtatatagc atttttccca caattaatag caggtcctat agttagatat gagagtgtgg
     1261 cagaacaaat tctcaataga aaagaaactt ggaataaatt ttctattggg acttgtaggt
     1321 ttataacagg tctaggtaaa aaagtattaa tttcaaataa catggctata gtagcagact
     1381 atatttacac tatgaatagt caaggagaaa tagctgcatc acttgcatgg cttggttcaa
     1441 tagcctatac tctgcaaata ttctttgatt tttctgctta ttcagatatg gcgataggct
     1501 taggtcttat gtttggtttt aaatttgaag agaactttaa ttatccatat atatcaaaat
     1561 caattagtga gttttggaga agatggcaca tttctttggg aatgtggttt aagagctata
     1621 tatactttcc gcttggaggt tctagagtag caaataaaga tattatgatt agaaatatgt
     1681 ttatagtgtg gttattcact ggaatatggc atggagcaga atggacattt gtaatttggg
     1741 ggattttaaa ctttgttttc ctaattatag agcgatttat aatgtttgaa aagattgaaa
     1801 atcacaattt tataaaacat atttatgctt tattagtagt taattttgga tgggtattgt
     1861 ttaggtcacc taatttaaaa gaagcatata attattttaa agctatgttt ggaggcaatg
     1921 gagctatatg gagtgattat acatatatgt ttttaaaaga atactgggtt ttctttatat
     1981 ttgcatttat ttttagtgta cctattgcaa agaaaatcaa taagtttgtt gttgaacaag
     2041 ctaaatatag tatgatattt aacgtatttt atccaatctc agctatttta ttgtttttta
     2101 tttctgtgac atatttagta actggaagct ataatccatt tatttatttt aatttttagt
     2161 tgggaggaaa agtatgaata gattttttaa agtaagatta ataactgcaa ttatgttctt
     2221 aattatttta ttcgctttct ctatctttaa tgtttgttat tctggaaaag atacgataca
     2281 ggaaataaaa aattcaatag ctgaaaaaag tagtttaaaa gatacaatat catctattga
     2341 taatacagtg aatgacaaag taatatacaa aaatacgttc attgaaactt atggctattt
     2401 acaaaaatta atgggtaaaa atgagttttc aaaatttagt gttgtaaaag atacttctgg
     2461 aaagatgcat tatacatatt ttgctaaagc tccaaatcca gttggtgtga tagcagatag
     2521 ggtaagaaaa ttttctgatg aaatggaaaa gcaaaacact aaattggttt acctaatgac
     2581 tccagataag tatataaaag gagtgacaaa atttccaaaa ggaatacctt attcttatag
     2641 taatgagaca gcagataatt ttttaaatca actaaaaaag tataatgtgg actatattga
     2701 ttttagagaa aatattttaa agagtggtat tccaaaagat gatttatttt ttaaaacaga
     2761 ccatcactgg aaagtagaaa cttctttttg ggcatttgga gaattagttg agcaacttaa
     2821 taaaaaatac aatatgaatt tagatgaaaa tcattattat agagataagg acaattataa
     2881 ttcaattgta tatcctaagt catttcttgg ttctatggga agaaagactg gtatcttata
     2941 tggtggtata gatgatttta cattaattta tcctaaattt aagactaatt atacttatta
     3001 tacagattct aaatctcaaa aatttgaatt aaaaggaagg tttgaagaga gtttaatatt
     3061 atcatatcct tttaatgcag accttgattt aatggatggt caatcagata agtattttac
     3121 atatttattg gggaacagac cattagttaa aataaaaaat atagaaaatc cagatggatt
     3181 aaaagtgctg tttgttaaag attctctaat tgtaccaaca gcagcatttt tttcctctgt
     3241 ttgttcatct atagatatga tagaccctag atattatgat ggagatatac tagaatatgc
     3301 aaaatcccat gactatgact ttgtttttat atctgtatat ccccaaaatc ttacaaaaga
     3361 attttttccg ttttgtgagt aaatttctaa aaaagttttc tgataaaata aatggctaac
     3421 tggtaaaaag ttagccattt attttgagtg ttattctaag aaatattttt tttactaaat
     3481 acttctttat ctatttttcc aagtgatttt cctacagtaa ttgatgatac catagcacca
     3541 ttaacattta tcatggtacg tcccatatct agtatagggt ctattgcaag tataccacca
     3601 actaatggaa aataagaacc aagaccaact ccagagataa caactgaaac ggccattgta
     3661 gcagttcctg gaagcccagc tattccaaac gaactaattg ttataacaat taacaacata
     3721 acataaaagc tcatatccat gtttactcca gccatattgg caagtgttac agccattaaa
     3781 gcaggatata tacctgcaca accattcata cccatatttg cacctaaact tgctgtaaag
     3841 cttgatacac cctcatcaac tccgtgattt tgtactagac tttctatcgt tacaggcaat
     3901 gttcctaaac tagaacgtga agtaaatgcc agaagtaaag cttcagttga attttttata
     3961 taagtaatgg gatttagacc atttaaagtt ataaaaatta aatgtataat aaataatatt
     4021 gcaactgata tataaagagc taatataaac tgtaaaacag atactattga actaatacct
     4081 tgtgaagtta ttgtatttgc aagcagagca accacagcat aaggcataaa tttaataaca
     4141 gtcatagaca cacttacaat tattttgtaa gatgcttcta taaaatctat aaatggtttt
     4201 attgtctcaa aatgtttttt acttaatctc tttattgagg tagctataaa tgtagcaaat
     4261 attattgtag ctacaatatt tccatttgcc atagccatta caggattaga tggtagtaat
     4321 cctcttaaag tatccacaag agagtttatt tctttaagtt cttgattttc aactaaagta
     4381 ggcatattag ctcctacacc taacttcatt atatttgcaa cgataattcc aacaattgca
     4441 gctatagcag tagtagcaag aagcatacct aaagatttaa atgtcagagt tccaagattg
     4501 ttatctttca tatttataat tacttttata atggataaga aaactaaagg tactactaac
     4561 atttttaata agtccataaa tccagaacca aataggctat accacttact tacttcttgt
     4621 agccaagtga cttgagttgt atcattagga aaaccagcaa aagcttgtat tatgattccc
     4681 aatatgagtc ctagtactgt agcatatatt gttctcaatg aaaattttat ttttttcttt
     4741 tcaaattgtc taacaataaa gaacgtacct agaagtaata ttatgaaaaa aattgtttta
     4801 aaatctgtaa tcataagaaa ttgtgataaa aatgaacttt ccaatatttt tctcctttta
     4861 tttaaaaata aatttttcta agtttaaatg atatcaatat attaaaaaaa tgtcaatatt
     4921 atatcacaat ttacttaaat agagtaaaac aagttgattt tgaaaaatgt gattaagtac
     4981 agaaaaatag ggtataataa atgtttaaag aatttgaatt gtggtaagaa aataaaaaat
     5041 agcttttata agatttgatt tcaaattaaa atatgttaaa taaataacta aaaattatta
     5101 taaaaaatgt tttcatagaa tatatatcaa atgaaaagct taaaataagt gaagaaatta
     5161 actagacatc aaaggagaga taaaatgaga gtaataataa ttggtggtgt tgcagctggt
     5221 atgagtgctg cagcaaaatt aaaaagaata aaacctgagt atgaagttgt tgtttatgaa
     5281 aaaactgaaa tagtatcttt tggtgcttgt ggattaccat actttgttgg tggatttttt
     5341 aatgatgctg atgaattatt agcaagaaca ccagagaaac ttagagaagc tggaattgat
     5401 ttaaatatat ttagagaagt cgttgaggtt gatagtgaat ctaagaaaat aaaagtaaaa
     5461 aacataaaaa cagatgaaat atatgaagat tactatgata aattgatgat agcaactgga
     5521 gcaagaagta ttatgcctcc tataaaaaac ataaaattga agaatgtttc tacattaaaa
     5581 agtttatatg atggagaata tctaaaaaaa cttttaagta atgaagataa taaaagagtg
     5641 acaataatag gagctggttt tatagggtta gaagcagttg aggcatgtaa gaaattaggt
     5701 aaagatgtag atgtaattca gttagaagat agaatattgc cacaagtatt tgataaggag
     5761 atgacggatg tattagaaga agaaataaat agacataatg ttaatttgca cttagatgaa
     5821 atggttgtag aactttgtgg agaagataaa gtagaaaaag ttataacaaa caaaggggaa
     5881 atagatacag atgtagtaat aattgctact ggcgtgagac ctaataccga gtttttgtca
     5941 aatactaata taaaaatgct aaaaaatgga gctatagtag ttgatgagta tggaagaact
     6001 tctgtagaag atatatactc tgctggagat tgtgcaacta taaagaatat agtaagtaat
     6061 gaaaatgtat atgttccttt agctactgga gctaataaat tgggtaggat agtaggagaa
     6121 aacctagcag gaagagaagt atcttatcaa ggttcactat cttcaagctg tataaaaatt
     6181 atggatatgg aggcagcatc aactggaatt acagaaaagc aagctaaaga tttaggcata
     6241 aatgtcaaat ctaaatttat ttctgattat aatcaaactc attattatcc aggaagaaat
     6301 aaaatatatg taaagcttat ttatgatgca gatacaaaag ttattttagg cggacaagtg
     6361 gctgggttta aggatgctgt ccaaagagca aatgtattgg cagctgctat ctttggaaaa
     6421 atgaccacaa atcaacttgg aatgttagat ttatgttatg ctccaccatt tgcaagaaca
     6481 tgggatgttt taaatgtagc tggaaatgta tctaaataat atttagatta tagctaatat
     6541 agttgacaag caattaaggg aatgaattta cctaaactat ttttatcatg gtaaaattca
     6601 ttcctaaatt tttattccaa atttttagaa ataaggattc cattaaatta ttcatcaaaa
     6661 tttaaacgca agcttaatct atcttctcca agaatagtat tttcaaaaac attagtagca
     6721 ttttctaaat acatactagg tatgtctaaa gaaggactaa aatgagtcag taaaagttgt
     6781 ttgacatttc caagttttgc aagattagca gcttctctaa aagtcatatg tttgtttcta
     6841 acagcttttg atatatctaa atcatcacca tacatggctt cacatataaa taaatcacta
     6901 tcttttatga aatctggtat tgattctata ggtcttgtat cggttgtaag acttatctta
     6961 actccctttc tcaattctcc taagaccata tctggagtgt attgttttga atctaaaact
     7021 atattttgac cttcttggag tttgttccat aatattttag gaacttcgtt tttagttgcc
     7081 ttatctatat caaatttagg gtttcttcta aaattaaaac tgtatcctaa acactctata
     7141 gagtgttcta aactgattgt tgaaatttct aaatttctta aaactttatt atctaaagaa
     7201 aaattgcctt gagggttttc tataattttt aaagtgtatg gtacatattc tactaaattt
     7261 ctcatagaac gtatacaatc gactattcca actggaccaa ctatagttaa atcacttgta
     7321 cgaccactat tcccaattgt tgataaaagt cctaaaagtc caaatatatg gtcaccatgt
     7381 aaatgggtta tacaaatcaa gtctatatct ttaaagccac atttttttag cttcatagat
     7441 acctgagtac cttcaccaca gtcaattagt atttttctac ctttatagtt tataaataca
     7501 gaggatagaa atctgtttgg cataggtaca tttccaccac atccaagtaa agttaaatct
     7561 atcatttttt cccctttcta aaattaacac aaatctgttg tttgttttta ttatgatatc
     7621 ctattataat tattttaaca ttaaatataa caataaatgt gtatttgagt caagaaatat
     7681 tgtcaaataa agtttttata tagaaatgta taaaaatttg gaaaacaaaa agtgaaaatt
     7741 atatttagga gcatttaaag gaggaaaatt taatgaaagt attaaagaaa attactattt
     7801 tgtcattgat tacattaacc ttagttggtt gtagtaacgg agaagataaa aaagtggttg
     7861 aaggttctaa gaaaattcaa aattctaaaa aagatgaaca gcctataata gtacataaag
     7921 atgcaaatat attgaaagat aggtatgagc caccaaaagg atttaaaaga gttgatgtag
     7981 aaaaaggtag ttttgaatca tttcttagga atactaagct aaaaaattat ggagagaaag
     8041 taaagtatta tgatggaaga actaaggaat caaaaggtat ttatgatagt gtatttgatg
     8101 ttgatattgg caatagagat ttacatcaat gtgcagatgc aattatgctt atgagagcag
     8161 aatacttgta tcagaataaa ctatatgatg aaataagttt tgattttgtt gatggtttta
     8221 aggcgaagta ttcaaaatgg gcacaaggat atagaatttc agtaaaagat agtggttcaa
     8281 gctggtataa agcaactgaa tattcaacat cttatgagag ttttagaaag tttatggata
     8341 tagtgtttgc atattcagga acgttatctc ttgaaaaaga attggagcct gtaaaagttg
     8401 aggatatgaa aataggagat gtgtttatag ttggtggaag tccaggacat gcaagtataa
     8461 ttatggatat ggcagaaaat gaaaagactg gtgaaaaggt atttatgata gcccaaagct
     8521 atatgcctgc acaacaaacg caacttctta ttaatcgtaa aaatccaaat ttgagtcctt
     8581 ggtatagttt agattttgaa ggtgatttga caatacctca atggactttt aaaagagacc
     8641 agttgaaaag attttaaaat aataatttta tataataaaa gaaaaatata gatgtgaaaa
     8701 aatgtaatta gattaaaact aattggatat ggacaaaatt aaattgatag ataaatatta
     8761 aaaattacac taattaatct tactatagtt taatatttat ctaaaaagtt ttatgtaaaa
     8821 tataaatttt atattaaagc taaaactaat ttgaggaggc taatttatat tgaagattag
     8881 aaggtattta attttggtaa ttataattct aattacaaca agtcagagtt ttatttctta
     8941 tgggctagag cgatttgaag aaataaccat ttttcacact aatgatatac atggaagata
     9001 tgctgaaggt gatgaccata tacaaatagg aaatctagcc acattgaaaa aagaaactcc
     9061 aaactcaatt ttagtagatg caggagattg tttgcatgga cttccaatag taaatatgga
     9121 tagagggaaa agtgcaattg aattaattaa atgtgctgga tatgactata ttactcctgg
     9181 aaatcatgac tttaattatg gaaaagatag actgtttgag ttatctaaaa gtgctaattt
     9241 aggagagaat actttaaaat ttttatcatc aaatattttt gaaaatggaa aaaaagtttt
     9301 tgatagtaat gatataaaag aaatagatgg aataaaaata ggattttttg gactctcaac
     9361 ccaggaaaca aagagcaaaa ctggatttaa aaatgtggaa ggattagagt ttaaaaatcc
     9421 tatagaaagt gcaacagaac aagtaaatga attaaaagga aagggtgcca atgtaatagt
     9481 tgcaatatgt catattggta caaatagttt aagtaagcca aatagtatag atatagcaaa
     9541 tgaggttcaa ggaattgatt tgattattga tggacacagc cacactaagt ttgaaaatgg
     9601 taaacaagtg ggtgatacat tgattgtaag tgcaggacaa tatttagaaa atattggcga
     9661 gataaaacta actttggata ctaatgatgt agaaaatata aaaatagaaa ataaatcagt
     9721 tagattaatt ggaaaagaag aagcattaaa gtataagctt gactccgctg taagtaaaaa
     9781 ggttgatgaa ataaaagaac aacaagaaaa aatattagat aaagttatag gggtaactac
     9841 aaatactcta gatggttcat atgaaaatgt aaggacaaaa gaaactaatt tgggtaattt
     9901 aataagtgac attttactag ataaaactaa ggctgatatt tctttattta atggagggaa
     9961 tattcgagat acgatagaaa aaggcaatat aacaagaaga gatattgtag atgtatttcc
    10021 ttttagcaat actatagtta ccaaagaact tacaggagca cagattaaag atgtattgga
    10081 gcatggagta aaactttatc cagaaaaaag ttcagccttt ttgcaagtgg gaggaatttc
    10141 atattacttt aatcctaaac aaaaagaagg cgaaaaaata acaaatattc aaaaagaagg
    10201 aaagccatta gatttaaata aaaagtatgt agttgcaacg aatgattata tagcatcagg
    10261 tggagatgaa tttccttgtt tttcaaaaga gccagtacta aaggaatttg gaaatttaga
    10321 gagtgcagtc attgaatata tagagtacaa aaaagaaata tcaaaaaatg tagatggaag
    10381 aattggtatt aaatctaaag atgagttaat aagcaagaat catgagagtg atttgatatt
    10441 aagtgaaaat gaaaaaaata tcaatgataa tgaagagaat ctatctaata atataaaaag
    10501 ctctgatagt gataaaaata aaagcttaga taaaacaagt tctagagaaa gttctaatct
    10561 aggaatatct aaaagagaag ctaaagaaaa atctcctaaa acaggagatt taggtttttc
    10621 aaatagtata attatattta tagtttcaag tacattgata tgtttattga attttaatca
    10681 aaaagagtta aaggataaaa agtcaaaata aaaattaatg agcaaaaact agtagatagt
    10741 aatttaattt tagataatag taaacagcaa aggaaattag ttatataaat actaatttcc
    10801 tttgttttat gttttaacat gattactttt aaataaaatt gtattttatg aaaaataatt
    10861 acactaaatg gcatagtatt atggggattt ttagttttta ttcaagtaaa tgaaatcata
    10921 ttaacaactt ctgatatatt tacaaattga taaaaaaatg attttattag tatgatataa
    10981 tttagctagt atgtatgata atggaggata tagggatgaa taatatatta cttttggaag
    11041 atgacaaatc tttaaataga ggaattagtt ttaaattaaa aaaagaagga tacaatgttt
    11101 ttagtgcatt tggtatagaa gaagcaaaaa atattttcgc aaaagaagag atatgcttaa
    11161 ttatttccga tataggtcta cctgatggta gtggttttga cttttgtgag gagattagaa
    11221 agaaaagtga tgtttacata ataatgttga cagctcttga tgaagaggtg gatattgtca
    11281 caggatatga tttaggtgca gatgattata tcacaaagcc ttttagcttg atggtattaa
    11341 tatcaaaagt gaatgcgctt atgaaaagag taaatacagt aaaaaattat actctattag
    11401 tttgtgatga tttattcttt tattatatag aaaataaatt aattataaga acagatgata
    11461 aggaagaaga gataatttta agtaagacag agacaaaact attaaaatat ttgatggaaa
    11521 attctatgca gacattgacc aaagagcaac tgttagaatc tttatgggat agtagtggta
    11581 attttgtaga tgataatacg attgcagtaa atataagaag acttagacaa aaggttgaaa
    11641 aaaatccatc tgaacctaaa tatataaaaa cagtaagagg ggttggatat atgtggggag
    11701 aaaggagtat aaaaaaatgc taaaaaatat gaacctcaaa gatgttttaa gtgtgaaaga
    11761 tgattatata atgagaagag ttattgtata ttttgtttta tctgtactaa taataaccct
    11821 tgtatttata ctttttgaat atagcagatt acataaattg tatgaacatc aaataaaaaa
    11881 tactcaaaat attgctggaa tattaattga aaaatatcct aatgatgaaa tagatatagt
    11941 aaattctatt tataattctg attatagtaa ggtgtctact ggagaaaagg catttaagaa
    12001 atttggctat ggattggaaa ataaaatgtc aaatgataaa aattttagct tatacttaaa
    12061 atcttttttt aaggaatcat tttttatttt tttaatgatg tttatagtag ttataggtat
    12121 aatatattat tttataagat acataaataa aagattgagt aaaatttatt ttatagtaga
    12181 taagatgtca caagaaaact acttgaagga cactactgag tcagaagaaa ctggttatct
    12241 agtagaatat aatgaatact ttaaagaagg catatttagt aagataaata actgtctata
    12301 tgagcttaat agaagtttaa agataaagtt tattaaatta gaaaaagaaa aagaaagtgt
    12361 aaaatcatta gtaacagata tttcccatca attaaaaact cctttagcat ccttaaaatt
    12421 atacaataca ttacttatag aagaagaatt agaagaagat gaagccaacg agttcttatt
    12481 aaccaataag gcttctataa ataaattaga aaatttaata aattctttag tgaacatatc
    12541 aaggttagaa attagcatga taaacataaa aaaagaagat aatgatataa aaaatactat
    12601 tctaaatgca gtaaaaagtg ttacccctaa ggctaagagc aaaagtataa cattaaattt
    12661 aaatgaattt aacagtagaa ttataccaca tgataaaaag tggactgagg agagtatttt
    12721 taatgtatta gacaatgctg taaagtatac acaggtaaat ggagaaataa atatatctgt
    12781 agaagaaact gctaatttct ttaaaattat aatagaggat aatggcatag ggattgaaaa
    12841 aaatgagttt aataatatat ttaaacgttt ttatagaggt tctgctgatg aaattgaatc
    12901 tgtagaaggt agtggggtag gattgtattt aagtagaaag attttagaag aacaaggtgg
    12961 aaatattata gtttcttcaa aagtaggaat agggagcaag ttttctttgt ttcttacaac
    13021 tatgtaatat taaatgaaag gaaaatgtaa gcctctacat ttattatata agtataaaca
    13081 gctccagaaa taaaaaatta ataaaatatg gggtaatata acttagaatg agaggataat
    13141 tttatgaaaa tactatatac tgaaaattta tcaaagcatt atggaaaagg agaaagctta
    13201 gtaagagctt tggacaatgt tgacttagaa ataaatgaag gtgaatttgt tgcaataata
    13261 ggaaaatcag gttctggaaa aagtacactt ttgcatatga ttggaggtct tgatatacct
    13321 acatctggga aggtgtatat agataataaa aatatattta cattaaaaga agaagaactt
    13381 gctgtattta gaagaagaaa aatagggttt atatttcaaa gctataattt aattccatct
    13441 cttaatgtat gggaaaatgt agtgttgcca atcggtttag atggtagaga agtagatgaa
    13501 tcttttataa aagaactttt aaaatcatta ggacttgaaa ataaacacga cgttttacca
    13561 aatacattat caggtggtca acaacaaagg gtggcaatag caagagcatt agctacaaga
    13621 ccagctataa tacttgctga tgaaccaact ggaaacctag attctaaaac gtctgatgag
    13681 gttatgagta tattaaaatc tatgtctaag aagtacagtc aaactctagt tatgataact
    13741 catgatgaca gtatagccca aatggctgat agagttatat tcatagaaga tggcagggtg
    13801 tcaaaggttg gtgataaaaa tgattaatac tactaaaata gcaaaaagta atttaaagca
    13861 aaataaatca aaaagtattc taataataat tactataata ctttcaacct taatgctttc
    13921 ttctattgga atttatattg ttgatgcagg agcatatcaa aaagaaaata ctataaagta
    13981 ttcaggcaat tatcaaggta tattagcaaa tgtagatgaa aaacaggctg atatattgag
    14041 taatcatgca gatgttgaat taactggtga aatgaatgga gtaggtgttg aaaaactgga
    14101 agatgattca aatatttctc tagcatacat gaatgaagat gctttgaaat taaatagttt
    14161 tgaatttatc aaaggaaaat tgccatcaaa agagaatgaa attgtgttag atagtggagc
    14221 tttaaaggca cttggttata aaaataaaga tttaggtgaa aagataaaaa tatcttatga
    14281 tgactataaa agtgataaaa aaattgaaaa agagttcatt ataagtggaa tcttaaaaac
    14341 tagcgaaata tctgaagcag gtaaatatta ttatgctatt atatcagaat catatatgag
    14401 aaatactaga gatatgtcgc aagaagattt taatatttat gtgaaattta atgataaaag
    14461 taatttatca atagagcaag caaaagaaaa attagataaa attgctaatg atataggact
    14521 ggatactatt aatactgctg taaatgaaaa ttatataaat gctttaaaac cagatatgga
    14581 aacgattatg ggtggagttt ttgtagggtt agtaattgta ctatctagta ttttagtaat
    14641 atacaatata ttttatattt caatagttac aaaggttcaa gaatttggaa aacttagagc
    14701 aattggtgca actaagaaac aaataaaaaa tatagtgttt aaagaagggt tcatattagc
    14761 cggaatagca ataccaatag gaataatttt aggctatgta cttgcaaata ttataataaa
    14821 atcatttatg gatatagatg taaaatcatc acgattacca gttatattat cggttgtggt
    14881 aataagcttt atatctgttg ttttatcact tttaaaacca atgaaagtag catcaaaagt
    14941 atccattgtt gacgcagtaa gatatacagg aaataaaata agtaataaga gtaagagaaa
    15001 aggatataaa aatataaatc taaagagatt atcacatgcc aatttagaaa gaaataaaaa
    15061 acgtacttac atgacattgg catcacttat acttagtgga actatattta taacagtatc
    15121 aactgcttta gaaagttttg atgctgagaa aatggcaaga gaacactttc cttatgatat
    15181 agaagttaga ctatctggtt atgaaatgaa cagtgataaa aatcctaaaa ataatttgaa
    15241 tattttacaa atgaataatc ctctaagtaa agattttttt aatcaaataa agaatatgga
    15301 aggaattaaa agaatagaat ctgcaagaag tgtaaaaatt ggtatggagg actatgatgt
    15361 cgaatttaag tatgaccttt tacaaagtat aaatgaaaat gatgtaaaat cattaaacaa
    15421 gaatataata gatggaaaaa taaatcttga aagacttcaa actggagatg aaatagtaat
    15481 tactcatgtt gatacagcaa aagaaatggg agtaaaagca ggagataaaa taagattaac
    15541 tttatatgat ggagataaaa agataaaaaa agaatttaaa gttcaagcaa tagctatggg
    15601 ggttccaagt tttggtatag gaaaggattt tattgataga actttgaaat acgattcaac
    15661 tagtagcttg ggaatatata cagaagaagg aaagtatcaa gaaatcaaag atagtatcaa
    15721 gaagattgct aaaaataatg gatttttaga gactgatttt atagatagta gaattgagtc
    15781 aaataaggct actatttcat ttataaagat aatgggatac acgctaacag gcataatagg
    15841 tgtgattgga tttatgaatc ttgtcaacac aatgataact agcatagtta ctagaaaaaa
    15901 agaacttggt atgcttcaag ctattggtct tactaataaa caattagtga aaatgcttaa
    15961 ttcagaggca atgtattaca cttctggaat gatgatagga agtattctat ttggaggtat
    16021 attagggtat attgctgtta tatttctaaa aaagactgga ttatcttatg caacatattc
    16081 tttgccaata gtaccaatct tgttgatgat tgtgtgtatt cttatagctc aatttataac
    16141 aacttatttg ataggacgaa gttttaataa agaatctctt attgatagag taagatacag
    16201 tgaataaaat gcaaatattt atggttagtt taactagcaa aaaacatata aaaaaatata
    16261 gttaaagaga ttcataatta aataggttaa agcatagtac agagcaaaca atttaaacag
    16321 atgtttgctc tgtattttat tatgtaatca ataatatatc taatatactg atattattca
    16381 tgttagaaaa tgtaccggta caattctaaa aaagtataaa attgtaccat ataaaatgaa
    16441 aattttgtat aataatcgta tagaaaataa ataggagctg atatatatga ataagaagat
    16501 taattatgca ccaggaccaa ctgaaactag ggaaaatgta agattagcaa gagcagaaaa
    16561 aacaacaaat ccagatatag atattgattt tgttgaattt tataaaaaga catgcgagaa
    16621 gtttggtaat ataattggaa caaaaaacga tgtttacata ttgagtggtg aaggaatatt
    16681 aggtcttgaa gcagcatgtg catcacttac agaaaaagga gatagagtac ttgtaattga
    16741 taatggtata tatggagaag gttttaaaga ttttgtaaca atgtatggtg gggagtatgt
    16801 gcttttttca agtgaatata ctaaaagtat agatattgat gaattgaaag catttttaga
    16861 tgaggatagt aattttaaat atgcaacagt agtgcattgt gatactccaa ctggcgtatt
    16921 aaatgatgta agcaaaatat gtccattact taaagaatat ggaatattaa ctgtagttga
    16981 ttctgtagca ggtatggtag gagaaagatt aagtgtagat gaatctaaaa tagatataat
    17041 attaggtggt tcacaaaaag ctatttctgc accagcagga cttactatag tcggaataag
    17101 tcaagatgca aaaaattgta taaaaaatag aaaaactgat gtaataggtt tttattgtaa
    17161 tttaaatata tgggaaggct attatgaaaa aaaatacttc ccttatacaa tgccaataag
    17221 tgacataatg gggcttgata aagcacttga taatatatta gaagaaggaa tagaaaatgt
    17281 attaaataga catgaaaaga tagcttatag tgttagaaaa tctattgagg aatatggtct
    17341 agaactattt ttagaagaag gatattctaa tacagtcaca gctataaaag ttcctgaaag
    17401 tataggagct ttagaactta ctgattatat gcttaaaaat tataatacac ttgttgcaac
    17461 atcactaaat caatatatgg atactatttt aagaataggt catatgggag agaatgcaaa
    17521 tctaaataaa atagaacatg tactaaatgt tttggataaa agtttaagtg cactaggatt
    17581 caaaggaaat ggaaatttgt taaatttatt taataagtat tatttttaat agataggggg
    17641 atatagtatg gaagttacag ctaagcaaaa atttacaacg aaagacatgg tggaaacctc
    17701 actattaata gcattagtat ttattgcaac taaatttata aacataagat taccaatatc
    17761 tataaatggt gggcttgtgc atttgggaac tgcaatgttg ttcatatcag caatagtatt
    17821 tggaggtaaa aaaggtgctt tatcaggagc tattggtatg tcactttttg atttgatatc
    17881 tgggtggact ttatgggcac cgtttacctt tatagttaga ggagttatgg gttatttact
    17941 tggaaagatt gcatgggcaa atggtaataa tgggaataat tttttaatca atgtaatagg
    18001 aatatgtgtc tcatcaattt ggatgctatt tggatattat gtaacagaag ttatacttta
    18061 tggtaatttt atagtacctc ttacttcaat accaggtaat ttaatgcaag tattgatagg
    18121 tcttataata gcgcttccta tttctaaggt attgaaaaaa tgtataaaat aaatataaag
    18181 tgaactaaaa tttatgatag cataaattat actgtataat attaggatgg agtacataat
    18241 taaaaaattt tagtatagag gttagttcac aatgttatca gttacaagat attcaatcaa
    18301 aaatgttcgt ttaaagccgt gggtaaagtt tatttggtat tttgagacaa aatcaaatat
    18361 actgttaaat aataagttac ttccgacaga cagtatagat attattctta atctttctga
    18421 ttttatggaa tataaaattg aaaatcaaga ctatactgct agtaatatgc attttaatgg
    18481 aataagggat aaacatggat ttattattca acatggaaat atacgtgtta taggggtttc
    18541 tttttatcca tttggtttat atccattttt aaagatacca atttcagagt ttaacagaca
    18601 aattgttgac ctagaagcag tatcaaaact atttgcaaaa aaattagaag agtcattaag
    18661 tccaacacaa tcagtagaaa aagttgtttt atgtttagaa gaagtattat tatccatatt
    18721 agaagaagat ttaatttcaa ataaatatgt aaagcttcta aattcattta tatatgttaa
    18781 tagatatagc aatataaagg cattttgtga tgatacaaat ataaatatta aaacattgga
    18841 aagaatctgt ttaaaatata cagggtatac acctaagatt ttaaagagaa tttacagatt
    18901 taaaatggca agcaatcaac ttatttataa ttataaagat gatgaacttt ttgattttat
    18961 ttatgaaaat gagtactatg atcaagcaca ctttataaaa gaatttaaaa aatttt
//
LOCUS       DAFKMX010000068        18961 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFKMX010000068 DAFKMX010000000
VERSION     DAFKMX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.068"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(78..2048)
                     /gene="ftsH"
                     /locus_tag="LZB08_001874"
     CDS             complement(78..2048)
                     /gene="ftsH"
                     /locus_tag="LZB08_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBM7315378.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2082..3470)
                     /gene="tilS"
                     /locus_tag="LZB08_001875"
     CDS             complement(2082..3470)
                     /gene="tilS"
                     /locus_tag="LZB08_001875"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBM7315379.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3653..4081)
                     /locus_tag="LZB08_001876"
     CDS             complement(3653..4081)
                     /locus_tag="LZB08_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBM7315380.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4098..4904)
                     /locus_tag="LZB08_001877"
     CDS             complement(4098..4904)
                     /locus_tag="LZB08_001877"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBM7315381.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5106..5591)
                     /locus_tag="LZB08_001878"
     CDS             complement(5106..5591)
                     /locus_tag="LZB08_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBM7315382.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5642..6301)
                     /gene="spoIIR"
                     /locus_tag="LZB08_001879"
     CDS             complement(5642..6301)
                     /gene="spoIIR"
                     /locus_tag="LZB08_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBM7315383.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6446..7126)
                     /locus_tag="LZB08_001880"
     CDS             complement(6446..7126)
                     /locus_tag="LZB08_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7315384.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7110..8000)
                     /locus_tag="LZB08_001881"
     CDS             complement(7110..8000)
                     /locus_tag="LZB08_001881"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBM7315385.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8118..9668)
                     /locus_tag="LZB08_001882"
     CDS             complement(8118..9668)
                     /locus_tag="LZB08_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBM7315386.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9762..10028)
                     /locus_tag="LZB08_001883"
     CDS             complement(9762..10028)
                     /locus_tag="LZB08_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBM7315387.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10176..11036)
                     /gene="yabG"
                     /locus_tag="LZB08_001884"
     CDS             complement(10176..11036)
                     /gene="yabG"
                     /locus_tag="LZB08_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBM7315388.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11120..12058)
                     /locus_tag="LZB08_001885"
     CDS             complement(11120..12058)
                     /locus_tag="LZB08_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBM7315389.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12168..12329)
                     /locus_tag="LZB08_001886"
     CDS             complement(12168..12329)
                     /locus_tag="LZB08_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315390.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12339..12836)
                     /locus_tag="LZB08_001887"
     CDS             complement(12339..12836)
                     /locus_tag="LZB08_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBM7315391.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12951..13661)
                     /locus_tag="LZB08_001888"
     CDS             complement(12951..13661)
                     /locus_tag="LZB08_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBM7315392.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYALGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFGYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13837..14367)
                     /locus_tag="LZB08_001889"
     CDS             complement(13837..14367)
                     /locus_tag="LZB08_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315393.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14384..14614)
                     /locus_tag="LZB08_001890"
     CDS             complement(14384..14614)
                     /locus_tag="LZB08_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315394.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14629..16023)
                     /locus_tag="LZB08_001891"
     CDS             complement(14629..16023)
                     /locus_tag="LZB08_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBM7315395.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16149..17402)
                     /locus_tag="LZB08_001892"
     CDS             complement(16149..17402)
                     /locus_tag="LZB08_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315396.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17453..18607)
                     /locus_tag="LZB08_001893"
     CDS             complement(17453..18607)
                     /locus_tag="LZB08_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7315397.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVVGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
BASE COUNT         5858 a         3613 c         1864 g         7626 t
ORIGIN      
        1 taatttaata ttatactttc agttttagta tttaatccac atcagataat aatcttaaaa
       61 tgaccaaggt cttaaaacta tttatcttta tcagatttat cttctaaatt tttatttata
      121 tcaataacat tgtttctttc ttcttcatct ttttctaatt ttatagttaa ttgttcttca
      181 acttctttag aagaattttc ctctgtaact gcattttcaa tttcattcag aggtaactct
      241 ttattaaatg cttttacaaa ttgttctgca tctaaagttt catatataag taaagcttgt
      301 gctacatact ctaatctatc catattttct tgtaatatac ttcttgtctt atcatatgca
      361 ccatcaacta tacgctttgt ttcttggtct atctcaaaag caacttcttc agaataattt
      421 ctcttacttg aaaaactatt acctaggaat acttcatcat cactgtcaaa tgacattgga
      481 ccaagtttag aactcattcc atactttgtt accatacttc ttgcagtagc tgtaactctt
      541 tctaagtcat ttgaagcacc tgtagataca tccttaagta ctagctcttc tgctactcta
      601 ccaccaagta aaactacaat attttctttc atttcatttt ttgtagcata gaacttatct
      661 tcaacaggaa gttgcattgt aaaccctcca gctcttcctc ttggaactat tgtaacttga
      721 tgaacaggac ttacttcttc aagtacatgg gcacaaactg catgccctcc ttcatggtat
      781 gctgtaagtc ttctttcttt ctcacttatt actttcgatt ttttagccac acctgctata
      841 acctttgtta tagcttcttc tatagtttcc atcttaattt tcttttctct ttttctagct
      901 gttaatattg cagcctcatt cattaagttc tctatatcag caggagtaaa tcctggtgtt
      961 cttctagcca agacatccat ttttacatca tcacttaatg gtttatttct tgagtgaact
     1021 ttaaatatag cctctctacc ttttacatct ggtgtaccaa caacaacttg tctgtcaaat
     1081 cttcctggtc taagtaaagc tgggtcaagt atatctggtc tatttgtagc tgccatgatt
     1141 attatacctt gatttacccc aaatccatcc atttcaacta gaagttgatt aagagtttgc
     1201 tctctttcat catgacctcc accaagacct gcacctcttt ttctaccaac tgcatcaatt
     1261 tcatctatga atatgatagc tggagcactt ttcttagcct gttcaaataa atctcttact
     1321 cttgaagcac caaccccaac aaacatctca acgaaatcag aaccacttat actgaaaaag
     1381 ggaactcctg cttctcctgc aactgctctt gatagatatg ttttaccagt tccaggaggt
     1441 cctaccataa gcattccttt tggtattctt gcacccaatt caatatattt cttaggattc
     1501 tttaagaaat ctactacctc ctgcaaatct tctttttcct catctagtcc agctacatct
     1561 ttaaatgtaa ctctagtttt ttcatcatct ttatgaacct tagcttttga tttgccaaaa
     1621 ctcataacct ttccgcctcc accttgagac tgattcataa acacgaacca aataatcacc
     1681 atgaagaata ttaacagtaa tgtaggcaac atttcgacaa accaaggtgt agatggctta
     1741 gcttcaccac caaaagttag tttcccagcc ttagcttggt ctaacacctc atctgcaagt
     1801 ttatttccca tcacttctgt tggaacatat gacttaaact ttgtatttgt atcttttata
     1861 gtaccttcta cagaagtttg atttacaaag tataatcttg atatattctc atctgttaat
     1921 tctctatata cttcagagaa ttttaaatct tttatttttt ctgtaggctt acctgagaat
     1981 tgaactatac ctactataat tataaaaaca agtagataaa aacctgcccc ttttagcaac
     2041 ttattcaata tagaagccct cctttcatac attgctttgc cctataattt gttaaaatta
     2101 atttttaata cttcttttgt gttttcatca attttataat tttcactgat tttataatct
     2161 ccaacacata ttattccttc actatctgtg attataggaa ttttacatct ttcttctctt
     2221 gggattttta aatctatgaa taaatctttc acttttttac ttcccatagc aagctttatc
     2281 ttatcacctt gccttctgct tcttattact ataccccctt ttaccttatt aaaatcaaac
     2341 cctttagatg aattgtctaa tttcatactc ttatacctat ctatgctcat tacttgggtc
     2401 tcaataattt gttttaattc ttttatttta ataaacccat tactaggtac attgtaataa
     2461 aattcaattt cctcattaac aatttcctca ttcgttaata ttatactgtc tttttttcta
     2521 taaacaaata tatttcttgg aagagttaac attttattta cttttgaatt atcttctaaa
     2581 gacattatat cctctatatg tctttggtca acaaaattag tatctcctag tatatgtttt
     2641 atggaatttc taagaactct aactttgata gcatcatgta agcaaacaaa atcgtctaaa
     2701 tttatctcta caaagccttt ttcttttata tttgaaactt ctctaaattt agcttctgcc
     2761 tctttctcaa tataatcgtt atcacttttt aaactattgc tcatcctcac tatagattct
     2821 attacatttg aattaaaatt atccttcata tatggtaaaa ggtctaacct tatcttattt
     2881 ctagtgtata tattttctaa atttgtttta tctatcctcg gatttaaatt ataagcttca
     2941 caatactcct ctatctcatt tctttccacg tctaatatgg gtctaattat gcaattatct
     3001 ctaatataat caattccctt taagcctttt aaacctgttc ctctcatgat acgcatcata
     3061 accgtttcgg cttggtcatt gagattatga cctattgcta ttttattggc tttaatctta
     3121 tccttaattt cataaaacat ttcatatctt agcttccttg ctccttcttc taatgataat
     3181 ccctcatttt cacaatattt tggtacatta atagacttta caaagaatat aattcccata
     3241 tcctcacaaa gtttagatac atataatgca tctttttgag cttctatccc tcttatttga
     3301 tggtttaaat gtgctgcata tatttctata ttaaaatctt tttttaatct atttagtaca
     3361 tgcaataaac aaactgaatc aggtcctcca gaaagaccta aaactatttt atcgcctttt
     3421 tgtattaaat tatgtttatt tatagtactt agtaccttat caaatatcat atttatccct
     3481 cttacactta gttttattca cttttaagta tactttctat aaaaaaacgc taaacccttg
     3541 tatcaatttt aagaaattaa agtttaatat aaatataata ataccaaata aattaaataa
     3601 aaggtagttt tttcactacc tttataaaat ttctggaaaa tagccatagt tattatttag
     3661 tacctatgta tatatctatt ttattcctac ctaaaaataa atcttgtacc tttatattat
     3721 agtctatttt caattttatg tgttcccctt cttgtatatt tatatctaat tctcttgtat
     3781 caatatcaat taataaagta ccttgtgggg tcacatactt tgtagtacta ctttctccac
     3841 atttaaacag cattgttgag ttaatagcac caaacttttt gatactaact tgatttttaa
     3901 gtatctttat agtattagtt atttctatat cttcttcttt ttccttgtaa ataacatata
     3961 ttccatcatt tttataaaaa attttaccaa aggcagtcat ctctatggtg tccatatttc
     4021 ctttttcatc atattgtcta gtatttatac ttatttttac actcaacgat ttttcctcca
     4081 tagattcttc atataaacta gtatttttgt atttctactt tttgaatctt atcaattttt
     4141 ttctttaaga actctcttgc tatatctgaa aatttttcag gtatatctga tacataatat
     4201 tgataagttc catgaatttc tgtattgttt ataatatttt gcacctctaa aatctttttt
     4261 aaatcctttg cagtctcttt agctggatta acaagtttta tatgctctcc aacttcttca
     4321 cctattgttc tctttaaaat aggataatga gtacatccta acactaaaga atctatattt
     4381 ttttctttca attcttgtaa atatatcttt gctgtcagtt tagccacttc tgtatttgcc
     4441 catccttctt caacaatagg cacaaataaa ggacatgctt tattaacaat ttctatactc
     4501 tcatctattt tagatatttc caagttgtat gcttttgaac ttatagttcc ttcagttcct
     4561 attattccta caattttgtt ttttgtagaa cttacagctg ttctagctcc tgcttctata
     4621 actccaatta tcggtatatc atatttttct tgagcttctt ttaagcttct tgcagtagca
     4681 gtgttacatg ctataactat agcttttaca ccttttgttt ttaaaaagtt tatagcttga
     4741 aaagtatatt taattatagt ttctttggat cttgaaccat atggtattct ggcagtatct
     4801 ccaaagtata ttatatcctc atttggtaat atcttcatta tctcttttag aactgttaat
     4861 ccacctaatc ccgaatcaaa aactcctatt ggtttattgt tcatctttat acaacaccta
     4921 ctttacattt ttgtgtttaa caacaactta tttatcgcta aaatttatat ttatattcat
     4981 aattaatata ttattaccta aatgattatt atagtttatt ttacacaaaa aataaaggag
     5041 ctatcttaaa ttagaaaata atttctacaa aaagacaact cctttcacta cttaaaaatt
     5101 taaatttatt ttgcaaacac atgatttcct attctcatat aaggattcaa tctatttatc
     5161 catttgcaag tggctatttc tggattccaa aaatatactg ctttattagt tgggtcagtt
     5221 ccataaagag cttcctgagc tgctttacgt gctgagtctg ttggtgccat ctttattgaa
     5281 ccatcaagca caactgagaa agcatttttt tgatatatga cttcttctat tgtgtctgga
     5341 aatctagggt ccaatactct atttattact acagctgcta cagcaacctg tccctcatag
     5401 ctttctcctc tagcttctcc tgcaaccaac ttagataata acatcaagtc attatcagat
     5461 aaatttatta cttctttctt tgctatattt ttttcttgtt tgtttacttt cttaatattt
     5521 tctgttggtt catatgcaaa tacttcttta ataggcataa atataaaagt caaagctaat
     5581 agtgtagcca tcagtttact ccacctgtcc atgattaaaa ccactcctta cattcttatt
     5641 tttaaaattt tttctctaaa tccttgacta cttcaagtac tttaaactta atttttactc
     5701 tacttgtttt ttttggtgta tcttgtttta ttaacttata ttctttatca gttaaaactt
     5761 ccttcaactt atcatctgta gatttatcta tcactccatt agattcatca acaaaacata
     5821 gtggagggaa cataacacac caccagtttt ttccttcacc tttcccaata ataatcttta
     5881 atgctttata ttcaccagct ggaagaacta tattagaata ttgtttagta gggaaattgc
     5941 tatattgtat acctaccttt acactgtaat tatatccatt tttaagaata atatttttac
     6001 ttatttcttc cagactacta tattcctttt ttataatagc ttcactttct tttatacttt
     6061 ttgaattttg aagctttggc tgtaaatatt ttattacttc atctctaact tttagtttaa
     6121 gttcttggtc ttcatctgta ttgctgtttg ctataacatg aaatcttatt aatttatctt
     6181 tataatcttt tgatatattg tctacttttt ttacctcacc atttattact attgtcatta
     6241 ttgatatgac tgatataaga cttaatatta atagtcccaa tctaatctta atttttctca
     6301 ttgaaaattc cccctcaaga taaatttata tcatcaatta tgtccaatta aatttaattt
     6361 attcataata ttttttaata ctaataaaaa aagctatctt aaaatgatta caatcatttt
     6421 aaaatagctt tttaaacctt atattttata agtctttact aatcatagcc ttatctatca
     6481 taaactgctc tgctttttct atatgctctg tagcatactt cttggatagc tcactatcac
     6541 ctttaattat agcatcaagt aagtatttat gctcttcaac aatattatgt gatgtatcat
     6601 aagcagacat ataggtaact ctaaatctat atacttgttc ccacaatgag tttattaatt
     6661 ggattaattt tttattatta gttgctttaa atatacattc atgaaattct acatcaagtt
     6721 ttaataacgc atctacatcg gattccaaag tgcttttttt aaattcttct gatatatctt
     6781 taagcttttt aatatcttca tcactaattc tttcagcggc aagatatgct gctaagcctt
     6841 ctaggcttgc tcttacttct aatacatcca taatatcctt taatgacata tttgccacat
     6901 acgcaccttt tctaggcaac ataacgacta aaccttctaa ttcaagcttt cttattgcct
     6961 ctctcactgg agtcctactt actcctaatt gttctgctag ttgcacttcc attagccttt
     7021 gaccaggctt taattttcct tctaagattg cttctcttaa attctcaaag actacatctc
     7081 ttaatggctt gtaattatcc aagttcagtt tagttaaatt ctccacaaat ttcaacccct
     7141 ttttgactgc tattaacaac atacacctgt ttatattttt ttaataattc tttttttcca
     7201 attagagcat cttctttatt tttaaatagt ccaaaaactg taggaccact tccactcatc
     7261 atagaaccta aagcattatt tttcatcatt acttgtttta tatcacttat ttctttgtgt
     7321 ttacctatag taacattttc aagaatattt accatgcttt ctgatacagc ttttatatct
     7381 tcagatttta aacactctat caaatattta ttatttggtc tttttttaat attattcaaa
     7441 tctaatcctt gataaacttc tttagtagaa acgaataagt ctggtttgca tatcaatata
     7501 tttgtatcac atggtaaacc tttgatgttt gttagctttt ctccaatacc ttgagctaag
     7561 gcaggtcttc ctgatataca aaaaggaaca tctgcgccca aattaagtcc tatttctttt
     7621 aattcatctt cagataatct aagttcccat aggtgattta aacctactaa tactgcggct
     7681 gcattagaac ttcctccagc catccctgct gctacaggaa tatttttttc tatgaatatt
     7741 tctacacctt tttttatata aaatttattt ttaagtattt tggcagcttt ataaacaata
     7801 ttatcttcat ctaaaggaat atctaaacta gtgcttttta cctttatttc atcttcgtct
     7861 aattctttta tctttactat atcgtataaa tctatcgttt gcattatcat ttcaacaaaa
     7921 tgataaccgt cttgcctttt tcccaagaca tcaatggata aatttatttt tgctctactc
     7981 tttagtctta taaaatccat tctatcctcc ttgtttaaag aataatgcat cactaacaat
     8041 aatattatac tacaaaaaat aaaataatag ggtattataa taccctatta tacttaatta
     8101 ctttaaattt tttaatatta atctactaat acgacttttt tttctaaaat aagacactta
     8161 ccaggcttaa ttggttcatc aagttttata tcattaagtt ctgcaatttc attttctgtt
     8221 gtattgtatt tttttgctat attccagaaa gtatctcctt ctttgcatat atatacgatt
     8281 atgctaggag ctttagataa atcatataca ccttggtctt ctcccttgat aataaaattt
     8341 tctgctttct tatctaatgc ttcagtaaat ctcttaattt tgataatcag gtctatctgg
     8401 tccctattca aatcaacttc gactttatca atacatgctg tattaaacac agatgctgta
     8461 tcagttagat tatccatttc tatatcatgt tcaaatggaa tttcctcact aattttataa
     8521 accaatttta aaccttcaac tgggacaaat aaaatttcaa cttttatgat tccttgtata
     8581 atgcttttat ttccttcaat ataactattt tctatagaca tggttggaca aacgcttaca
     8641 atatctttta tttgtatatc atcattatca tttcttatgc cttctctaac cataaatgtt
     8701 tcttcactac gtcttaaagt cttatttaat tgaataggtt tgtggtcaaa ttttattatc
     8761 ttttgaggcg aatatgcatc ttgtaatact tctcttgtaa cttcatctgt aacttttacc
     8821 ttacaacaag ccatacaatc tatttctaaa agacctgtgt tgctttcact attttgcttg
     8881 aaaatatgat taaaatctga catagacaac aatacttctt cagtcatacc atcactaact
     8941 ccagggactt ctacaaattg agtaaactca atacctactc tatccaactc tacaagttca
     9001 ccctcatatg tacaagctaa cggatttatt tctaatactc caccaataat aactttattg
     9061 tcagtcactc tactttcttt tactttaaca catggattta aacttattat agattgaatt
     9121 tcctctgtgt ttattgttat agtatctctt atagaacttt ctgctttttc tattccaact
     9181 atatcttgaa aacaaatttc ctttctgtgc ttttgtatgc cttccacttg cgccacatct
     9241 tttacaatgt ctaacctttg cttttcaaac aaacttcccc ttatattcat aagagctcct
     9301 actttaatct ttctctcatt cattatagta caatccatat gctctacttc tgaaaataac
     9361 atgtattcca tatcttgaac aacattgtct ttttctatta cttcgtttat atctactttt
     9421 ccgtctacat tagaaatagt atttttatca tctgcaatat agattacatt ataattaaaa
     9481 ctacctctgc aaagtatctt cccatctgcc atttcaattt ttttaagtga tatgtatcct
     9541 tctgttttta caatttcata tacatctaat tttttgtctg gtacaactgc ctccgcttca
     9601 atgaatgttt gaaattttcc aaagtctatt ctattgtcaa ctttaattac atctttaatt
     9661 aattccatag tctacctcct taagtattat aaaaatatca cactatattt atatttaagg
     9721 agcaaaaaaa aaattacacc tatttttaaa taggtataat tttaacttat ttgtaattta
     9781 tcttgatctc taaaaacttg caactttaca ttagatgtta ataaatctga gtaactataa
     9841 gatactctag gatatccatt actttcatca tctaacttta taacaaacac gcttggataa
     9901 actttttcta gtataccctc ttttgtaata atctgctttc ttcctttatt agctttcaac
     9961 aatattttct ttcctatatg tctctctaag cttactctta tcttatctag agtttgaaca
    10021 gtagccacaa tatcaccttc tttaacgttt atatgtttac ataatatcac atataaacat
    10081 ttttgtcaaa tgtttaaact aattatttta tatatttttt gcaaaaatgt caatactatt
    10141 tttgaagata tacagtataa ttatgttaat ttttgctaat gtaatattgt ttttggcatt
    10201 gcccatctaa attttcctct tgtttcagaa ccaccaattc ccttaacacc tgtaatagtt
    10261 tgctctataa cctcatcaat aggaactact acatcaattc ttgaacaagc aattttctcc
    10321 actataaaaa cagggtctaa tgcatgtatc attattcttc caggcgaact tgcatagttt
    10381 gctcctgcac ttattatttt ttcataattt gattggcatg ctcctgcgaa tatgacaaga
    10441 ttatccaaat ttggttgcca tttacgtgct tcttttactg ttttaacaaa atttgaagag
    10501 tttctatagt tactcatatc ttctatatct cctcttttta ttgtcattgc atcatgacct
    10561 gttattacta gtatatccgg attatatttt tctagtagtg ctcttacttc tttatactga
    10621 ttttgctctg aaacagctac acctactgca ggtattccaa gttgagcata tacatccaag
    10681 caaattttta aatattcttt atctccatct atctgtagta cttttccagg cattccatat
    10741 gtgttaggat ttaattgaag ttttggaact gctctagtca ttcttttttg tctttcttta
    10801 gcttttctta ctgacttata taataaattt tctacatttt tatctattaa tatatctttt
    10861 atatcaggtg cttttactaa ttctaggtca tcaatataag catctgctat tactctaaat
    10921 gcaattccct ttagtatagc tattttctca ttattttcat ctacaccaaa agagactatt
    10981 ttaaatacta tatctttgtt atgtgacttt ctagctacaa tgtctcctac cttcatagtt
    11041 aaacctccaa taaattttta tcttagttta tactatgttc gatataaaac tttgtttcct
    11101 aatgtcaaaa aaacatttct tataatactt cttttatcac tcttaaagca tttttatagt
    11161 atatcttttc tatatcatct tcactaaacc cttctttttt tagaggttca tacagtttac
    11221 ccatctgaga tatatcttca atctcaactt ttgaatctat tccatcaaaa tctgacccta
    11281 gagatacaac atctattcct ccaacattta caatatgttt tatgtgtcta accatatctt
    11341 caagtttact ttcactttta tcacttaaaa ataagtggaa gaagtttata cctgtaactc
    11401 cacctttatt tgctaatact ttaatcatat catcagttaa atttctagaa tgattcatca
    11461 ttgctcttga attagaatgt gtagcaatta ttggtttaga agaaatcttt gcaatttcat
    11521 aaaatccacc atctgaaata tgagaaacat caaccaacat tcccaattca ttcatcttat
    11581 gtaccacttc tcttccaaaa tcagtaagcc ctttttctct atattctact ttattatgag
    11641 gaaagctcaa ttcattcaca tgattccaac ttattgtcat cattctaacc cctaagtcgt
    11701 aaaatttttt taaattttct aatttacctt caagtactgc accttcctca atagataaaa
    11761 gagctgtaag tttaccttct gattggtttt tcataatttc ttcatagtta gtagctaaag
    11821 ctatttcatc actatttttt ttcatttcct catgaaattt atttgccata ctcatgcaat
    11881 aatcaaaagg atgttttact tcttcagtat caacaaaaag agcaaatgtt tgagctaatg
    11941 aatccccctt tttcaatctg tctatatcaa cagaatattt attagactta agttcacttg
    12001 tttctacatt ttccattaat tttgctatag tatcacaatg taaatcaata aatttcatat
    12061 ttataccccc tgtatatttt atatttatat ctaaaaatag aaatagttgt ccttatagac
    12121 aactattgac aactattttt taatattctt tttaatatta cttaatctta ttcttctttt
    12181 aagttaggta ttactttttt aacttcttca atttgatgcc atgttttatc tatattattt
    12241 tcactaatat atttttttaa atcagttaaa gatgtaaaat ctttaactga taaattgtca
    12301 tcatcatctg caaatcttaa aataaacatg agactccctt actttccaca acatttctta
    12361 tatttttttc cgcttccaca tgggcatgaa tcatttcttc ctatttttgc ttctttaact
    12421 attgttttag acttcttata agcctttatt atttcttttc tttggtctgc tggtagtata
    12481 tcttcccaac cttgtaaatt atatagccaa tgagcctcaa cagttaccat attgttatat
    12541 aatttttccc aatctatttt tatagaaact tctttatcag cttctacttc ttctaaaact
    12601 atatcttctt ttaagctttc acttattcca tctatgaatc ccatgaaaaa ttcattagta
    12661 gttccaaatt tttcagccaa ttcacttact ttaccttcaa caacatccat tttattattt
    12721 aatatttcat tgtatattcc agcttcaact ttcaaatatt cttcccagaa ttttacttct
    12781 gcttcttgac tttcatgatt atcacttaaa tttctccatt cagtatataa actcatactt
    12841 tttcctccta agtataccta tatttttata gtttttatag tataaaatct tttagttaga
    12901 ttggaagttt cttctttttg attataccat attctattta aagttaactc ttatattata
    12961 tcataattaa acaattcttt taatactttt atagcatact cttggtttat aaaataaggg
    13021 cttgtcgcaa ttgttattac tttggctttt ttaattaagt tttttatctt gtaaaatttg
    13081 agttcaaatg gtatatagtc catatcactt gaaaatatat ccaaatctat gtcaagaaca
    13141 aactctgatt ctacttctaa atcaaatcca tatagactat ctattattat taattctgaa
    13201 aatatgttat attttaaagc aggttttata aaacttccca catttaaaac ttcatttgta
    13261 tatccaaata catcttttag attatttaca tctacatcat agttctcagg ttctctagta
    13321 tccttatgtt ggtctacatg cactaacttg catccttttg taaactcacc ctttaacaaa
    13381 gattttgccc aaaaataaaa tgcatggtta tgattgtcaa acacatatat ctttttattt
    13441 cccagggcat attcgaccat attctccaat ccttttgctt ttatttcctt attttcatct
    13501 acttcattaa atactacctc attgcctaac cttacatcat ttaatgtacc atttataagt
    13561 tttggaacgt atatgctctt attttctctt ttatcatatg aaaatacatt atttcctact
    13621 ggtttttcta tataaaatcc actatattca taattttcca taaaacttta tttcctccat
    13681 tgttatataa atatataagt tataatttta aaaattaaaa tgcacttatt gtaaatcata
    13741 tcttctatta tagaaaacat cttatataac taaatataat atcttctctc aatatgtatt
    13801 atacaataag tgcaaaatat atttatataa aatatactac attcctataa caaaaccatc
    13861 agcttcaatt ggaaggtaac taactcttct attcaactcc acctttccaa aaaatactac
    13921 ccaatatggt ctaaaaatac tttcaatttt aactatttca atctctggga ttcctcccat
    13981 aaacctatga gttaatctca tagccatttt atttgccttt gcttttactc ttcgttcgtc
    14041 cttatcacta tactgcacaa tattacaatc cacatttttt acaacttcat atgtaggaaa
    14101 atcttgtact atactaccag aacctgtaga accattcact aaaattttta attcatttat
    14161 atcattagaa tatctcctat tcttgaaaat ggatttcgtt ttgtgaattt taagtgtgat
    14221 aattttaaac tctatataat gaagtctaac ttcatgagca tatttgttct ttaatattag
    14281 cttactaaat aaagaatttt ttccactttt agctatctct atagcttctt cttttgtaat
    14341 tcttataggt aacgctctta tatccataaa atcattctct ttcttatttt tttcttgtca
    14401 tttttaaaac tctatatata taatcaaaaa atattacaat tacaactgcc ataactccat
    14461 atggaattat ttttccaact ataatatcat ttctagtcaa tataccataa aatataaatc
    14521 ctgttattaa aaagtacaat aatactagaa aaccgtctat tccaagccat tttatttcta
    14581 aatctttttt taccttagac actaatttag acatagcagt ttctcctttt atagattatt
    14641 ttgtgtattt aaattttcac ctattctatt accaatcatc catgcaataa atgaaaaagg
    14701 tatagctatc acaagaggct ctaactcaaa gctctgaact ggaaatattt taaaataacc
    14761 aaaagtcact ccaccaacaa tcattcctaa aataccaccc atagatgttc ttcttccatt
    14821 tccaagaagc ccacctacta taggtacaaa tgccgaaccc atccaaatac ttcctacaaa
    14881 tatacatgca tcatatacat tttcaaatct aaatgctacg ctaagcccca taactgctgc
    14941 taaacataca cctattctag taaactttac taattgtttc tgagataact ctttttttgt
    15001 aattcttcca tatatatcat ttgctaatgt agctccagct gctagataat agctatccaa
    15061 tgttgatatt atagaaccta aaattcctat tataaatatt actcttattc cttgtggtaa
    15121 tgtatctaaa actaaagtta tatatccagc agctggagcc atttctggat aagtagctct
    15181 tactataagt cctgtcatag ttaacaccat gtcaaaacat atccatagtg aaaaacaagt
    15241 taataaggct cttcttccaa ccttaggaga atctgctgct gaaaatcttt gataaaaagt
    15301 tgggtctgaa taagctccaa aagaatatac aattaacatc aatgctttca tagctgacat
    15361 accttttgta gggtgcataa gctgtggtgc atctttgagt gcttctgtaa gaccttgaaa
    15421 ccctcctatt tgttcaaatt gacctggcat aaccatagtt aaaccaaata ccatacaaaa
    15481 aaacaatacc atatctgtaa tggcaactcc cataagccca ccaagtactg taagtacaac
    15541 gactactcct accattatta atcctattaa ttctttatct attttccatg ctacgttacc
    15601 tatagttccc atagcagtta tttctccaag ttgtgcacaa tatataaaca tcaaaattgc
    15661 cccaattata gcaacatgct tgccataaaa actttccagt aaatcaggaa ctgttatatt
    15721 ggttcttata tgaatccttg gaccaaccca caatgctaaa ggcattctcc caatatgtgc
    15781 agctattgac caaactgccc atgcagatat tccatatata gccgcatact ctacactacc
    15841 tactgtacca actcctccat accatgatgc aaccaaagtt cctactatta aggaccatgg
    15901 taaacttcga tttcctaggt aataactttc catatctttt acatttctag aaaaataata
    15961 acctattcct aataatataa atgcaaatat aactataatc ccattttccc aaaatgttaa
    16021 catatctaat ctccacattc ttatatttta atttttaact ttaatatcta aatatgaaca
    16081 actctaccca tttccataaa aataggtaga gttatataat aattataagt atttaagtaa
    16141 tcttgttatt aagcaatttt attgtttaaa cctttatttt ctcttttatt tattttattt
    16201 aaagcataag tagcaaatat ccctataact gcacatacta cagataaaat aaatatatat
    16261 gtatatcctg tttgtccata tttatctaac caattacctg caatagtgta ataaaacgca
    16321 tctggagaaa atcctataaa tgaagcaaat ccaactactg ctcctgtagt ttctaaagat
    16381 atattagact ctgaaacaga tgcaaagtat atacctctaa atccaaataa aattacactt
    16441 agtattatca tgtttattac agctatccat ataaatcctg tactcttagg aataaccaag
    16501 taagcagctg tacttactgt cattaatata aatcctacaa acattactct tatagatgaa
    16561 ccaactttat ctgttataac accagcaact ggagatgctc ccatttttat aacataagtt
    16621 cttattattg ataaagcaga aactagagtc attggcatta cataaacttc tgataagtat
    16681 ggacttaaat aagttaaact tgagaatact aaataagttg aaaatattat aagaccaatt
    16741 aaccaagcct ttggcattgt aactactttt aacaatgact taatacttaa tccttcatct
    16801 gatgctccac tatctttttt ctcttctact aagaaaaata gaagtatacc acatattata
    16861 gatgctgcag aatataacca tactacatat ttgaatccta ctgttatttc agcaaattta
    16921 ccaaagaaat aaagacctat aaaagaaatt aatgtacctg atacgcctga taacccttca
    16981 taaaaaccaa atattcttcc ttgctctgac tcacttcctt gcattctaac aaccttaaca
    17041 gaggctgaga agaaggttaa tattgtcgta aatccccata atacaaatat taaaagtatc
    17101 atactatatg atgggaatgt agagaaataa aatccaagta ttcctgaagc aattaaagaa
    17161 aaagttatta attttctaac tgagaatcta tctgcaaacc atcctcctat aaaataagat
    17221 accattccta ctaatccata taaactcaat aagttcccta tttgtgtatg acttaatgct
    17281 aaagcttgtt gcattgggtc ataaaatgat gatttcatat atggtaaact atacatagct
    17341 gttgttccaa atgccaatat gaatattatc aaaaatcttt taacattatt tttgctttcc
    17401 attgattctc tctccttaaa attttattgt ttataatctc attcatgtaa ttttatttta
    17461 atttttttat tgcttgttta aagtcattta ttatatcatt tgaagattct aatcctacag
    17521 ctattcttat tagattttcc ccattcattt gaactaatgt atgctcttcc cctaagcttg
    17581 ttgctattgt tgcaagctct aaagaattca ttaatcttct actagcttgg tctcttgtca
    17641 atccattgat accatcctta agtttaaatg aaactacacc tccacctaaa ccattcattt
    17701 gagttttaca aagctcatat tgaggatgag attttaatgc tggatacttt accaattcca
    17761 cattctcttc ttcctctaaa aactctgcta ctgctaatcc attttcacaa tgctttttca
    17821 tcctcatatc taacgttttc atacctctta aaatcatcca tgcactcatt ggtggtaaag
    17881 atgctcctgt aaaacaacat aaacttggcc atctaactaa atctattaat tcttttgaac
    17941 ctactactgc tccccctaaa gtatccccat gtccatttat aaacttagta agactatgta
    18001 ttactaaatc tgcacctaaa tctattggtc tttgtacaat tggtgtccca aaagtactat
    18061 caactatagc taagctttta ttttcatgag caatttctgc tattgttttt atatctacta
    18121 agtctaatga tggatttaaa ggactttcta tataaactat tttagtattt ggtctcattg
    18181 cttcttttac attttgtgga tttactgcat ctacaaatgt aacttcaact ccaaatttag
    18241 ataacaactc agacatgaat aatttagtgt gtgaaaatat tccatctgaa ctaactatat
    18301 ggtcaccact ttttaccaat gctaatatag tagatgttat tgcccccatc cctgatgtac
    18361 tagatatggc actttctgca ttttcaagca tagccaactt agcttctaac tctgtaagag
    18421 taggattaca ctctcttgta tacacatatc cccatgtcat acacaactct tcaaaatgct
    18481 ctactgtctt tgcagcaaat gttgctgttt gacatattgg tgtagccaat gcacctgttg
    18541 ttgggtctgc actatgtccc caatgtatta tttttgtctc taaatcttga ttaatattgt
    18601 tactcatttt gatttcctcc cattttgact tatttataca acgttttccc tgccaaattt
    18661 catcaattta gcttagtctt aatacatttt ttcaagtata cttttttatt tgttataatt
    18721 tctaaaaata tgacacttat tttgtttttt tacagttcta attataaact ctatagtaac
    18781 tatagagtca atatgttttt gatatttttt ttacaatgtt taaaatagtc agtacaaaaa
    18841 taggtatatt ttagaataac taaagtcatc ctaagatata cctattttta tatcacaata
    18901 taaataatta catataagca ttagtttttt tatcataaat agatttttca agatttatca
    18961 t
//
LOCUS       DAFKMX010000069        18935 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFKMX010000069 DAFKMX010000000
VERSION     DAFKMX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.069"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(217..2562)
                     /gene="secA"
                     /locus_tag="LZB08_001894"
     CDS             complement(217..2562)
                     /gene="secA"
                     /locus_tag="LZB08_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBM7315398.1"
                     /translation="MSVIDSILDKADEQEIKKLNIIVDKIDALEDSMKNLSDEELKDM
                     TAIFKNRLEKGETLDDILPEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLDMTVGVIISNQD
                     PSTRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDKEEEQEVKDL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            complement(2827..4953)
                     /gene="slpA"
                     /locus_tag="LZB08_001895"
     CDS             complement(2827..4953)
                     /gene="slpA"
                     /locus_tag="LZB08_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBM7315399.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFANVVTGSEGYTVSQKDYKKAV
                     KQIQDGIKKNIITSINIYFDGTKVSTVNVAGDQDARDKIADDFNNTVEGKLDSLGDGK
                     YVDFEISYNKGIEEYTKTELENYKKLLDNKVVIPKASGVNPGAVKEKSGSADEAVAAD
                     NDIKGSDLYNTTVEADTTNGGYKLSITAKTISDVKYGTIGAGKYPNAKVITATGTDAL
                     VKGKTVDISASYALEASTGNVSGLSLTDTNPSSDSVNVRIINAKEITIDLDASSYDSA
                     ESLAKKYVFKPSTLTSIYTDIEAGNIERVNGKYQVEIFADGKRLNTLSAVNSTIADPD
                     STAKVIIKADKLKDLKDYVDDLKTYNNGYSNSTNVSGDDRIETAIALSEKYYNSDDDN
                     ALFEKEVNSVVLVGSNAIVDGLVASPLAAEKKAPLLLTSKDKLDSNVKSEIKRVMNLK
                     NTVGVNSSKKVYLAGGVNSISKDVENELKDMGLKVTRLAGDDRYATSLEIADEIGLDN
                     DKAFVVGGTGLADAMSIAPVASKLESGEATPIVVVDGKAKTLSSDASDFLGNAQVDII
                     GGENSVSKDVERDIDDATGKSPERVSGDDRQATNAEVIKNSDYYEKGTVKNFFLAKDG
                     STKEDQLVDALAAAAVAGNFGTKFDAAGGETSPAPIVLATDSLSSDQSVAISKALGDN
                     DGENLVQVGKGIATSVVTKLKDLLNM"
     gene            complement(5165..6754)
                     /locus_tag="LZB08_001896"
     CDS             complement(5165..6754)
                     /locus_tag="LZB08_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBM7315400.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGAPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDARELANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(6973..8574)
                     /locus_tag="LZB08_001897"
     CDS             complement(6973..8574)
                     /locus_tag="LZB08_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBM7315401.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSNKTV
                     LEGAKNITSVGEINEKVIQQCISALKSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYVLTLKATDEWGKSTGKRVEIKVLDDKSQDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(8941..10821)
                     /locus_tag="LZB08_001898"
     CDS             complement(8941..10821)
                     /locus_tag="LZB08_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBM7315402.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNHKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNI
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(11031..16994)
                     /locus_tag="LZB08_001899"
     CDS             complement(11031..16994)
                     /locus_tag="LZB08_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBM7315403.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDESNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKILTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKARMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAINISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(17118..17203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(17375..18778)
                     /locus_tag="LZB08_001900"
     CDS             complement(17375..18778)
                     /locus_tag="LZB08_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBM7315404.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         5340 a         3564 c         2093 g         7938 t
ORIGIN      
        1 agtttataac taatagactt ttcatcttct ttatagtata atattaaatg gaagaaagaa
       61 atttcaagca tctctttttt ccgtatttct cacacagaaa aagaagctga ttttcagctt
      121 ctttttcatt tgtattcctt aattatttaa ttgtaattac aatataaaga atcaaaatac
      181 tttatttttc atttaataaa aattacatga acttttttag ttaaatttat ataagtattg
      241 cactgttgct tcccttatat ttttatttaa agcttcaaac atgtcatatc cttctaaagc
      301 atattcttta aatgggtctt tttgagcata agatttaaga cctatatact gtcttaactg
      361 ttccatagca tctatatgat ctatccagta ttgatcaacc acttttaaaa gtactgtttt
      421 ttctaactct gcaaccttat caataccaat catcatcttt ttcaagtcat aaactctttt
      481 tgaaatttca tatgtgctat caattatctc ttggacactt tttttatcta caccaggtat
      541 tagtaatgta tctgctggca taaatgtact atataaatgt ttaaaatatc cataataatc
      601 tctttttctc ccaattaaat aagtttctcc tgcttcttgt atgatgtctt taaccatttt
      661 ttgaatatct tcttgtatat cttcatcatt taacacttta tttctttcat tatatataac
      721 ttttctttgc tcattaatag tatcatcata tttaagaaca ttttttctta tttcaaaatt
      781 tttaccttct acaccctttt gtgctctttc tatagctctt gtaagtgctt tactttcaat
      841 ggcagtattt tcttttgagc ttgttctctt cataagtttc tctatagttt ttccaccata
      901 aagctttatt acatcatctt caagacttac aaagaatctt gatgtacctg ggtctccttg
      961 acgaccagaa cgccctctaa gctgattatc aattcttctt gattcatgtc tttctgttcc
     1021 tataacataa agtccaccta aatctttaac ttcttgttct tcttctttat ctcccgcacc
     1081 tagagaaata tctgttcctc tacctgccat atttgtagca atcgttatag catctaattt
     1141 accagcttta gaaattatct ctgcctcttt atcatgttgt ttagcattta agacttgatg
     1201 cttaatacct tgttttttaa gcattctaga cagcttttca gatttttcaa cagaaactgt
     1261 tcctacaagt attggctgtc tagtcttatg tatccttatt atttcttcta caacagcact
     1321 atacttttct tcttctgttt taaatacctt atcatgtaaa tcagctctaa taactggttt
     1381 attagttggt atttggacaa catttaattt atagattgac tcaaattccc cttcttctgt
     1441 ctttgcagta ccagtcatac cagaaagttt ttcatatagt ctaaagaaat tttgataagt
     1501 cacagtagcc atagttttag attcattctt tatctcaaca ccttctttgg cctctatagc
     1561 ttggtgaagt ccatctgtat atcttcttcc atccattact cttcctgtaa attcgtcaac
     1621 tatcattact tctccatttg agataacata gtcaacatct ttttccataa gcttatgacc
     1681 tcttaaagct tgatttatat gatgatataa ttctatgttc tttatatcag taaggtttgt
     1741 tataccaaaa aatgactcag ctttacttat gccacttgct gttaatgcta tagttttatc
     1801 ttttctgtcc atctcaaagt cttcttcttt aacagttttt ataaaactat ttgctaactc
     1861 ataaagtttt aaatcttcat caccatctcc tgcaataata agtggagttc tagcttcatc
     1921 tattaatatt gagtctacct catctactat ggcaaagttt agttctcttt gtactttatg
     1981 agataaatct ggcaccatat tatctcttaa atagtcaaat ccaaattcac tatttgttcc
     2041 ataagttata tcacatttat attgttcttt tcttgtactt gggtcttggt tagatataat
     2101 tactcctact gtcatatcaa gagattcata aactgggcgc ataagctcct tatcacgttc
     2161 tgctaggtaa tcatttactg tgattacatg tacaccctta cctgtaaggg cattcaaata
     2221 tactggtgct acttcaacta gagttttacc ttcaccagtt ttcatctctg ctatctttcc
     2281 ttgatgtatt actattcctc caattaattg cactctatat tgacgcattc ctaattttct
     2341 ttttgatact tctcttacta cagcaaaggc ttctggaagt atatcatcta aagtttcacc
     2401 tttttcaagt ctatttttaa atatagctgt catatctttt agttcttcat ctgatagatt
     2461 tttcatacta tcttctagtg catctatttt atctactata atattcaatt tctttatttc
     2521 ttgttcatct gctttgtcaa gtatcgaatc tataactgac atataagtac ctccttttct
     2581 cttgtccaat tactattata cattaaaaat ccataatagt agacaattat tttaaattat
     2641 agtataatga ttttacttaa ataatcaaac atctttttat ttaagtattt ctcacatatt
     2701 aattggtaca aataaaaaaa ctcttaacaa ttattaaaac taatgttaag agttttttat
     2761 ttgataaata ttataactgt aaaaaagact cttcaatgaa gagccttttt attataaaaa
     2821 ttttgattac atatttaata aatctttaag cttagttaca actgaagttg ctataccttt
     2881 accaacttga actaaatttt caccatcatt atcaccaagt gctttactta ttgctacact
     2941 ttgatcagaa gataatgaat ctgtagctaa tactattgga gctggactag tttcaccacc
     3001 agcagcatca aatttagttc caaagtttcc tgctacagct gcagcagcta aagcatcaac
     3061 taattgatct tctttagtag aaccatcttt agctaagaag aaattcttaa ctgtgccttt
     3121 ttcataatag tcagagttct tgataacctc agcattagta gcttgtctat catcaccact
     3181 tactctctca ggagattttc cagtagcgtc atctatgtct ctttcaacat ctttagatac
     3241 actattttct cctcctatta tatcaacttg agcatttcct aagaaatctg aagcatctga
     3301 acttaaagtt ttagcttttc catcaacaac tactatagga gtagcttcac cactttctaa
     3361 tttagaagca actggtgcta tactcatagc atctgctaaa cctgttccac caacaacaaa
     3421 agctttatca ttatctaaac ctatttcatc agcaatttct aatgaagttg catatctatc
     3481 atcaccagct aatctagtaa ctttaagtcc catatctttt aattcatttt caacatcctt
     3541 agatatagaa tttacaccac cagctaagta aactttttta ctagagttta caccaactgt
     3601 gttttttaag ttcataactc tttttatttc agacttaaca ttactatcta atttatcttt
     3661 tgatgttaat aataaaggag ctttcttttc tgctgctaaa ggagatgcta caagaccatc
     3721 aactatagca tttgaaccaa ctaaaactac actatttact tctttttcaa ataatgcatt
     3781 atcatcatct gaattgtagt atttttcact taatgctatt gctgtttcta ttctatcatc
     3841 acctgaaaca tttgttgaat ttgagtatcc attattataa gtttttaaat catctacata
     3901 atcttttaaa tcttttaatt tgtcagcttt aattattacc ttagctgtag aatctggatc
     3961 tgctattgtt gaatttacag ctgatagagt atttaatctc tttccatctg caaatatttc
     4021 aacttgatac ttaccattaa ctctttcaat atttcctgcc tctatgtcag tatatattga
     4081 agttaatgta cttggcttaa atacatattt tttagctaaa gattcagcag aatcatatga
     4141 acttgcatca agatctattg ttatttcttt tgcatttata attctaacat ttactgaatc
     4201 actacttgga tttgtatcag ttaatgataa ccctgacaca ttaccagttg atgcttcaag
     4261 tgcataagac gcagaaatat ctacagtttt accttttact aaagcgtctg tacctgttgc
     4321 tgttataact ttagcatttg gatatttacc agcacctatt gtaccatatt tcacatctga
     4381 tattgtcttt gcagttatac taagcttata tccaccatta gttgtgtctg cttcaacagt
     4441 agtattatat aaatcagaac ctttaatatc attatctgct gctaccgctt catcagcaga
     4501 accagatttt tcttttactg ctcctggatt aactccacta gccttaggaa ttactacttt
     4561 attgtctaat aattttttgt aattttctaa ttctgttttt gtatattctt ctataccttt
     4621 attataactt atttcaaagt ctacatactt accatcgcct aaactatcta attttccttc
     4681 tactgtatta ttaaaatcat cagctatctt atctcttgca tcctgatccc ctgcaacatt
     4741 tacagttgaa actttagttc catcaaaata aatgttaatc gatgttataa tgtttttctt
     4801 tataccatct tgtatttgtt ttactgcttt cttatagtct ttttgtgata cagtataccc
     4861 ttcacttcca gttactacat tagcaaatac aggagcagcc gaagctaaca ctgttaatcc
     4921 tgacatagct attgctatat ttttcttatt catttcttaa attcctccca acattatatt
     4981 aaaattacat ctcttattat gtacatagaa taatcatcca tatattccta atatttctca
     5041 catctctata ataatccatt tttattcttt ttgcaaggca tttttttgaa attattattt
     5101 ttcccaagca tatgagtttt tataccctta aatttcaata tttaataact taaaaaattt
     5161 aattttactt atttaaaaat tcttgagtag catacacttc tccattaggc aatatatata
     5221 atccaaaccc agtatataca aaatcactat ttaacatatt ttcattatat ttatctgact
     5281 ttttccaaac tgtaaatatt acatttgcta gttctctagc atcttttgtt gtataagtgc
     5341 tctttgtaac aagtggtaca aaagctatat tttcttcact tctcaaccca tatccagtaa
     5401 atacttcaac agatttttta ccatctatta catgtgaaaa ttggccttta tctgccatgt
     5461 acttagacca ggaattagaa agcccttcta agtttttaga aacttgtaat ggctctttac
     5521 ctttttcttt tctatatgaa tttaccaaat tatacatttc actagaaacc attcttttaa
     5581 actcactact attcatatca ttagtcttat attcaagtac attcacttct acacttattg
     5641 aggtagtctt tccaccaata tcagtagctt ttatagtaac tttgtattta cccaccttat
     5701 cagtattaat atttcctata agctctggtg tcaaatcgtt accattgcta tcctttgcta
     5761 ctatattaag tttacttata tcaaacttat caccttgata aatatttaaa tttccaacag
     5821 ttattgttgg tgcacttgca tccagtgatg atgcacttaa acattgttct agtgtctttt
     5881 catctattcc tcctagtgct gtgattttat cagcacttct aagtacagac ttatcactat
     5941 ttccatcaac caatacaata ggtgcatttt tagctataga agaaccagca actgcatcta
     6001 ctaattgata acctttagaa agataaaatt cttttgctga actataaaaa tgttttataa
     6061 cttttttatt agtctcaaat ctatcttctc cagctaatct aactgcatta gtttttttaa
     6121 ctaaatcatt acttattatc tcctctgaac caagagcata gcattgcaca ccttcttttt
     6181 tctcaaatgg aacattttta ccattagtta aaattatagg tgctccatct cttgatgcta
     6241 ctgatgacgc actcatagca tctgcttcac ctgtataacc attagttata aaaaccttat
     6301 ctataggttt tattgaagct atttcttttg ctataagata acttgtttca attctatcat
     6361 taccacctat tcttttaact tctatccctt tttgctttaa ttcattttca actgattttc
     6421 ctattgaatc ttcagttcct attatataaa ccttctttac atcttttaac attttttctg
     6481 tgtctgatgg tatactgttt ctttgtgata ataatatagg tgctttaact gttcctgcta
     6541 atccacttgc acttaatcca tcagctagtg tattatctgt atttactaat actacatttt
     6601 catatgtttg tttctgtgca attttggcag ctgtctcata tctatctttc ccctgtatct
     6661 tttctatact tgaaagtgca ttgatatttg gtgctcctgc cattattaat gatattgtca
     6721 atcctattga taatactctc ttatttacct tcatcaaact cctcctacta atttttttat
     6781 ataattcagc taatcataaa ttacatcctc agtagtgatt ccctcaaaat ctataaacta
     6841 ctaaaacaaa aaacacctaa aataccatag cctgtaaagc aataacaaaa cagattacaa
     6901 aaagtacttt agatgtgatt ttcattttat aatatcagta aattaacaaa aaaacaagat
     6961 ttatggaatt ttttacaagc taccttcatt tccatttaaa aactcctgag tagcatgcac
     7021 ttgaccatca gataatatat aaagcccaaa tccagtacta taaaactcat tactaagcat
     7081 gtttgcattg tactctggag acttcttcca cacctcaaat atagcctttg ccaagtcttt
     7141 tgcatcttgt gtcgtttgaa cgttcttaga atctatataa atatatgcta tattttcctc
     7201 acttctcatt ccaaattcag aaaatacttg tggagcattt tttccatcta tataatgtgc
     7261 aaatactttt ttatccatca tatacttaga ccatgcattt gccatacctt ctagcctact
     7321 agacactacc aaaggctctt tgcctttttc ttttctatag gaattgatta agttatacat
     7381 ttcagtagat accatttttt taaattctgg gctgttgtaa tcctgagatt tatcatccaa
     7441 cacttttatt tctactcttt ttcctgtact ttttccccat tcatctgtag cttttaatgt
     7501 caatacatat gtacctactc tatttgtatc tataaaccca tcaacttcta ttggtaatac
     7561 cttccctgtg ttatctttag ctactatatt taacttgcca gtgtcaaact tttcaccctt
     7621 atatacctct gtacttccaa ctgtaattgt tgggggttgt ccatttgact ttaaagcact
     7681 tatacactgt tgtattacct tctcatttat ttcacctaca gaggttatat tcttagctcc
     7741 ttctaataca gttttattgc ttccatcatt tactagtacc attggagagt ttttagtaag
     7801 tggtgcagcg gctattgcat ctactaattg gtatccatct gatacataga atccatctgc
     7861 actattaaaa aagtagtcta ttacgttttt gttagtttca aatcggtcag ttccttctat
     7921 tcttactgag tttgtatttt taactagagg attactcatt atctcctctg aacctataca
     7981 atatgattga atatttttta catcaaaagg cacactcttt ccatctgtaa gtatgattgg
     8041 agctccatct ctagtagcta ctgatgatac actcattgca tctgcttctc cactatatgc
     8101 attagttaat agtactttat ccactttttt tatagttgct atttctttag ctattaaata
     8161 gcttgttttt agtctatctt ctccaccaat tcttttgact tctatatttt tagaatctag
     8221 ttctttttct actgatttac ttatagtatc ttctgtacct attatatatg cttttttgat
     8281 attttttaaa catctatttg tgtctgctgg tatcttattt tgttgtgtaa atagtattgg
     8341 agcttttgta gctcccgata atccacttgc acttaatcca tctgcaagtg atttttctgt
     8401 atttactaaa attactgttt cataagtctg tttatcagct atctttgctg ctgtttcata
     8461 tctatcttta ccgtatattt tttctactac tgatgatgca tttacacttt tgaagtttac
     8521 taagattaat gatactgcta gacctaatga taatattttt ttgttcattt tcatatcccc
     8581 ctgatgattt ttatatttta tatttatatt gtgattattt tccatttatt ttctctttat
     8641 tttccatata ttatagagta tatatgatat gaatatatct cgtctatata ttagtgtttt
     8701 attttattaa atttaaaaaa ttatttttaa cttcttttta aacatattca aaattgaatt
     8761 tgtatttata tatataaaag atatctgctt tatttaaaat taaacttaat atagctcaaa
     8821 ttattataca ttttaacctc aatattatta aattgttaag ataatgaaaa taaaataaga
     8881 atgcatttaa atatataact ttatatctaa atacattctt attttctatt tacttaatat
     8941 ttatttttca attaaactct taatcttatc tattacagat gaggctattc ctccacctac
     9001 ttcaaacaac tttgatgaat ttttaccttt taatgacgat tcttgtgatt tatctaacgt
     9061 atttgtagca agtataactg gtgaattgtt tttagctgct aatggtccaa ctgctaaagc
     9121 atctataagt ttgtcttgat ttgcaactac aactccatct agcttagatt gagagtagaa
     9181 tttatctatt actttggcat tcgtctcttg tctagtttga cctgccaccc tattgtttga
     9241 tatatctttg tttgctattt ttccaacttg ttcaatagcc ttgttagata ttttttctac
     9301 tccaccaata aaatatatat ttgacacctt atttgattta ataaattgtt cagcttcatt
     9361 atctacacca tctttttgtg taagtactat tggagttttt tctttaccag ccagtgatgc
     9421 tattgataat gaatcagctt ctccatttcc tgctcctata tacaacttgc ttatattatt
     9481 cgtcttatct atttcttttg ctatattaat agaagtttga tatcttgtat ctccacctat
     9541 tctttgtact gatatttttg aatttacagc tttgatagct tcaacaactg aatttggcat
     9601 tgagttgttt ccacctataa caactacttt tgatggattt agccttttca attcttctac
     9661 agttttttga ggaagctttt catttgatga taaaagaatt ggtgagtttt ttacactagc
     9721 aagtggtgta gctgtaagcc catccaccat acttttatct tctccattta ctattacaac
     9781 tgtatctgat tttgtccaac cactttgaga tattttgata gctgtttcaa atctatcagc
     9841 accttttaat tttttgactg tgacattaga tgtagaatca cttgaatttc ctcctccaga
     9901 gcttccacca ctagatgagc cacctgaatt acttcctccg ccattatttc caccattgcc
     9961 attatctggt ggtaattcat ctggttttga taattcattt tcaactatgt attctaaact
    10021 ttttggaagt gtaacttcaa catatttgtt tattgtagct tttcttccct tagttgtttt
    10081 ttgtgctaat ccaagtttta atattacatt tgaaccactt tcttgtgttt ctttactaac
    10141 aatagaatag tttatcctaa tattgtttaa taaatattca agtgcttcac ttttctctac
    10201 cttatacttt tcagggtcat ttttactaga accagttcca ccaattggtt ttacttcact
    10261 ctcattaata agggtaaata ttaatccttc atcagagctt atattttcat aatccactgt
    10321 tttgttgtta tatgttacaa tttttaaagc tggtttatgg tttgttcctt ttcctaaaac
    10381 tttatagtca gcatttgagt tactgccaga tttttttaca gatattcttc ctatattcaa
    10441 tctatttccg tctagtaaat cttcatttga tgtaacatat atatctaatg tagttgtatt
    10501 acttgatttg ttgtattcta ctctacaagt agcatttgca tttccacttt ttatctgaga
    10561 ttttatatta tcagaccaag taatcttttc tttctgctca ttatctacta cttcaacctc
    10621 accttgtact tttatactgg cttgaaatga atatactatg ttttctactg agtccacaga
    10681 tatatctata gtatttactt tctcatacgt ttttgttcct tgtaaaactg gagctgcata
    10741 tgcacatgta gcactaccag ttacagctac tgccatagtt gatactgcta tagttcttat
    10801 aaaagatttt cttttactca ttaacacccg tccttatcta tcattaacta agtggataaa
    10861 tttacttatc cacctagctt ttatatttaa tttataaatt accttaaact tatagttaca
    10921 tttatatact ttacctttaa tatctactat tttaaaacta ttctagtacc taatcttatt
    10981 agatatacaa tgttagtgtt agtattttta cgtttttaga gttccaccta ctatttatct
    11041 ctaacaatat taccctttac tttattgctt acagttataa ccttcttatt actgatatta
    11101 atagcatttc ttgtagcttt tctagcttta ctattactat cagtaagact tattgtcttt
    11161 aactcatcat aattcttagc tcctacaggt acatacattg tatcacttac aaatctttga
    11221 cctactggag tatctccttg aaggtcattg tatctatata gttcagcctt aaccttatca
    11281 cttaaagaat caaagttttc ttttggcata tagtaaaccc atgtaccttt actgatttca
    11341 cctagttcac ctttttctgg aacttctgca aataaaagtg cttctccaga tatagtctta
    11401 ttgtttttat ctataagtag tggtaaatta aatgctggat ttccactgta ttcacctgta
    11461 ataactattg aacctgctgg aatcttacct gtttcatctt cacttgtgct agagtcatca
    11521 ctttgatata cataatcttc tgctaatttt ccaattccat tagtacgagt ttggtctgca
    11581 ttattcattg tgccaatttc tatattatca cctggaggtc ctattatatc taattctgca
    11641 acttctatat tagacttatt atttgagatt agttttacat atctagcttg gtatgtccaa
    11701 agtttaccat ctttattaaa tgtaactcta gcttcattat cttggtcttt tcctccacca
    11761 actaaatctt gtccatattc aaaagttcct tgacttacat cttcccagtt ctttttatca
    11821 ttacttactt gtatcttgta attagttaaa ggattgtatg ttgtactagt attctttcta
    11881 aagttaaata atccttttaa tgagaacttc ctagcttctg cctctggttt tgtatatttt
    11941 agtcctacta taggtaatgt ttctttcatg tctactatta tatatggatt ttcttctaca
    12001 tttatatcag ggtctttttg agggtctttg ttccactctg ctttacttat tatctttcct
    12061 ttatatgtag tacttgcatc attatctttt atattatcaa tttcaggatt ttgtaatggt
    12121 ccatgtgagt cattttcagt tctaacatca ccgtcagaaa cagtatttgt tatgaatgac
    12181 catgctgatt tatttatttt cccttcatct gataatttta cagtaccaac ttcaaattct
    12241 tctgttggat ttaggtgata atcatatgca actaccttat atgtaaatgc tctgttatta
    12301 agaggagata tattatcagt aaatttagtc ttactatttc cttttgcatc cacaaatcca
    12361 actgttctgt attttacctg tgttccattt tcaccatcta aataatttcc atcacttctt
    12421 attatttcgt atcctagtat cttatcttta tccttatcta catctaggtc tatagatatt
    12481 ttattagatt taactagaga accactttgt attccatcag aaaaactagc acttacttta
    12541 gtatcttttg ccatactagc tatattttca tttaaaatct ttcttctagc ttcgtcattt
    12601 acatattgta tctttcttga ttctttttcg tagttcttac tatccattat actgtttaat
    12661 tctggtgtaa tttctactcc ccatgcctta aagtaatctc ttaaatcttt ttcaacaaca
    12721 cttgatgcca ttgctactag catttggtct tttggtaaac tattaattgg gtcactactt
    12781 gaaagtgctc tgtattttct tgaaatcttt gcatagaatg tatcattact taaatcagca
    12841 tctgagttat tctttaacat tgaacccgta aaatcatcat cataagctag atgtaattgc
    12901 cataacattc caagcctttc aaatacattg cttggtatac ctaagctatt tgaagttaca
    12961 tgctcatata tcttatccat tgttgaacct tcaagtcttg aatgtgtctt atcatcaaag
    13021 gtctgagcaa gtaaagcaag tacattgtta ctagtttcac tatatgtcat ctttccaata
    13081 tctgtaacat gtcctatctc atgacctatt ccccatccaa ataagtttcc tcctgtagct
    13141 tttccactat catctatttg gaaaggtact ccattcataa gccctggaac tgaaccatat
    13201 tctactccta catgtaatcc agaagcatac ataaatgctc caataaacat tctttggtat
    13261 tttatgttca tacgagcttt tggagccata tcatttttag ttaagttaaa ttcattatca
    13321 tctacagttc cattaccatc taaatcttgt gttttaaata ctcctctttc tgagtaaact
    13381 aaatccatta tttgttccca tgcaagcata ctattatata atctatctac ttgtgtattt
    13441 aaatcagcat ttccttttga tatcccttga tatacagctg ttgcaggaac atttaatgtt
    13501 actttatcac tttcaatatc tgtagtgttt aacacaccag ttttttctga atatccatat
    13561 gaattaccaa catttgttcc tggataatat ggttctcctt ttacagcatc tttatattta
    13621 gctaattctt ctatatatgc ttttatcttt ttcttagatt cagaaactcc cgcaggactc
    13681 ttatcattta ttgttccatt tagattaaga tgtggtatct tagtagctcc tgctacacgt
    13741 acttttatat ctcctccact tggagtatct tcagcgtatc ttatgtataa acttcctcca
    13801 cattctatat ctaaactatg aagtttgctt aattgaatta cattttttcc agtgttaaga
    13861 tttatttctc cactcttata tgcaccagat tctccatagt actgtgtata tatcaactta
    13921 ggagtttttc cacttttact tccaacatat actgtgattt catctcctgc tctagcagct
    13981 actcctaatg cctgccagtc attttgcata cctaaattat tggaatttac agtatactgt
    14041 ccatttttat ctttatattt tcctaattta tttatttctt gatttacagt agttacatca
    14101 gtaagggtgg ctctatcatt atataagtct tgagctaatt ttaattctct ctcaataact
    14161 gtcttatatg gatgatattc tccactagct ttatctggag tatttaatct atcttttaat
    14221 tcagttattt tttcttgagt tacatcttct tttaattcaa cttgtaggtc atccataaat
    14281 aaatttctta catcatcttc tatactgtca tactcataaa acttaagttc tgatatatta
    14341 gacttatatg aataggatga gcaaacactt acttgtatct tggtagcagt tattggttct
    14401 tttaatctaa ccattacata cttattatta tttacagggt ctgtctttgt ttcaaaagga
    14461 acatccccca gattgtgtaa ttgaccattt tcatcccaat atcgtacctc aaagtcattt
    14521 attgtcttat cagaaacatc aaaacgaggt gtagccataa aagtatccat agtataagat
    14581 ttatcaaaag ttattattgg tcctctattc gttccctcag ctgcattata tgttgggaat
    14641 gtccaatgtg ttgtaaagtc cccatctact acattgaatt tatcaaatgg atttttatca
    14701 ctatattcac ttttgtcata tcctgctgga taagtaacat ctacaatgtg atttgttggt
    14761 attccaaatc caccattctc tttaggagta ttaattaatc tatagttagg tgttgttggt
    14821 gcatccatgc tttcagttgt acctatatat gttttagaca ttccactagt tcccaagtgg
    14881 ttagtagctg tcattctaac ttcatatgtt gtattatcct taagaccttc tatctttgaa
    14941 cttgtcccac gaataagctt atcattgtat tttatccaag acccattaga gcctttttct
    15001 ttatagtaca agtcaaaatc tttagcttgt ttatgatttt tccagcttac atcaagagat
    15061 ttaaatttac ctttaacatt tataccttct ggtggttctg gttttgtgtc tggaacaact
    15121 tgaaattcta atatactatc tgggctaaat tgtataggtt tatagtttga atctacattt
    15181 tctggtactc cgtctaagtt ttctggtgtt gcatctttat aaccactaga ccaatctcca
    15241 tttaaagatt gtatgctaac tctatatagt ccatatgctt ctatatctct aacttctatt
    15301 gtatttttac tagtttgtaa ttttttagta gataatactt ttcccttact atcaattttg
    15361 tctagtataa gttcgtatgc tgatacattt acttcattat tccaataaaa agatactttt
    15421 tcatttccaa cagcagtgct agtcaatatt ttatttattt taggtatgtt catctctggc
    15481 tttggtattt ctttatatac attgtttaaa aattcaactt ttgatatttc tgcaagattt
    15541 ctatcgtttg ctgttgtagc tgttattttt atagttatct tttttactgc tacttgttta
    15601 ccaagattta ttactatatc actattttga acatctgcat atgtttctaa tgctgaccta
    15661 ctatatgtag atgttgtaga gttatcatct ttactgatat taccctcttc ttttggagta
    15721 tcttcatctg aattaggttt atccattgat gaattttctt tatcattatt tttatcttga
    15781 ttggtattag agtttacttc ttgtttatcg ctattttcct gttgtttatc attactttca
    15841 tctttattat ttgagttatt atcagtctgg tttatatttt catctttatt atttacttct
    15901 tcttgattta cttcttcttg ttcgttattt acttcctctt gttgctcctc atcttttaag
    15961 tttttcatat attctttgtt ttcataagga atctgttgaa cattaccatc cttgtcagta
    16021 acctcaacta ttccttttgc tggagcagta ccttctggag cttttatatt gatgacttcc
    16081 atattaagtg cttctgataa agctatatcc attactgctg gtttttcctc agatatttca
    16141 tcattttttg aagatattgt aactccatta ttttctccac taaataaatt ttctacatta
    16201 ccattaatat ctacatcact atttcctttt aattcaacct tacttgtatc tactatagga
    16261 ttggtagata ctatactttc taggcttttt gcatcttcac tttgtcctaa atttttctgt
    16321 acataatcta agtcaacaat atctaccttt ccatctctat ttaaatcaaa ttctctttta
    16381 tttgaatcta tattctctaa aaccttatta taatcactct tatctacttt accgtctcca
    16441 tttacatcac ccattaagaa caattcataa tttaaaggtt tatttttatt atctacactt
    16501 gctctattgc ttaataagac tctttgtgaa taacttgata tatcaatgtc tttaatttct
    16561 ttgttcttaa atccagctcc agagacttct acattgtatt ttccaacagg taaattatta
    16621 aatacaatat tataatatga tacattctca tctttatttt ttatttcact tctacttgca
    16681 gataattttt ttatagtata ttctatatta tctgtcccaa catttactgt tcctgaatct
    16741 ttttctccaa gctttattat tccactattg ttactatcat cttttaatcg taactgcata
    16801 ttagtcatac tttctgactc gctatttttt attggcagtg aaaaatttaa atctacttca
    16861 agactccccc ttaacttttc tgtaactgat ttttcaactg cccttagctt cccctctaat
    16921 ataggttctg caaaaacgtt tagtggtgca ctacacatag atactgctag tacacaagat
    16981 attgcctttt tcatgaaata acaatcctcc cttaataaat atctaatttt tcatgaattt
    17041 aaatactata agtttcagac aataaaaaaa gacactcaaa gagaatctta attataaaat
    17101 aaaaagactt tactcttttt agcaactggc tgatatggct ttaaaagctt taatccctat
    17161 agttttgcgt cattagattt ccctaatttt gccatttaat tttcactact ttcttaccca
    17221 ttttatacta tatttaaact ttttaataca atttttttac attaaattat aaaaaaataa
    17281 catttatatt aatttattaa tataaatgtt attttccatt ataatttaat tatttatata
    17341 gcttccttac aagcataatt ttgtgtatat tttcttattc tttatatctt ccataaaatt
    17401 catgtgtagc atatagattc ttatctcctg ttatagctat accaaatccc attatttcat
    17461 accctttatg caacatatta gtgccttctt ctggatttgt cttccactca ttaaaaataa
    17521 agtttcctat atcatctgca tctctatttg tatatacagg tttatctgga attttatatt
    17581 taattatatt ttcaccttga actgctatat atccaggttt aatctcactc caatctaagt
    17641 atttcaaaaa agtagaatat gaattttttc catttatcgt atgagataaa gtcttcttat
    17701 ttgccattaa attagaccaa tcatttgcta gaccctctaa tctgcttgct acagataacg
    17761 ctcttactcc attctctttt ctatactgct ttgtaagagc atatacttta ccctttattc
    17821 tctctttaaa tgctctatca tttggattgt aatataaacc ttgttgattt ggtgaagctt
    17881 gatttataca ttgcttgata acattctgat ttatgcctcc tagtacagtc aatttgtctg
    17941 caccacttaa aatactcttg ttactgccat tttcaactaa aactacagga gcattttttg
    18001 ctattgtaga ggcagccaaa gcgtctgtta attgtaagcc tttacttaga taaaattctt
    18061 tactattttt ataaaactct tgtataacta tcttattagt ttcaaatcta tctgtgccac
    18121 ccaatctaat agaatttgtg ctcttaacta attcgtcgct gattatttca ctagaaccaa
    18181 gcgcataact ctttaagcct gtcgtgtcaa atcccacact atttccatct gtaagtatta
    18241 taggtactcc atctctagca gctactggtg atgcacttat cgcatctgct tctcctgtaa
    18301 atccattggc tataattact ttatctattg gcttaatttc tttgattttt tcagctattt
    18361 ttatacttgt atcaaatcta ttttcaccaa atattctttg tacaaccttc tttttggata
    18421 gtaactgatt ctcaacattt ttacttattg ttgattctga acctattata tacacagtct
    18481 ttgctttgtt taatctttcc atagttgtgt ttggaatctg gttttgtttt gtcatcaaaa
    18541 taggcgcatt taaggctcca gctaaaccac ttgcgcttag accatctgct aagctattat
    18601 ctgagttgat tagtattgct gtattgtagt tttgcttatc tgctattttt gctgctgtct
    18661 cgtatctgtc attccctttt atttgctcaa ctgatgtcaa cgcatttacc ataggagtat
    18721 ttaccattac taaaaatagt gtaagtccta tagctaatat ctttttacta ttcttcatat
    18781 tattttctac ccccaatgta cttttcatat tttttattag tactcatact tatttttaat
    18841 caaaagttac aattttgtta ccatatttta tatatgatac catagttatc catacaactc
    18901 aaacaatata cctaaagttt ttattctcta taaat
//
LOCUS       DAFKMX010000070        18870 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFKMX010000070 DAFKMX010000000
VERSION     DAFKMX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.070"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            71..1357
                     /locus_tag="LZB08_001901"
     CDS             71..1357
                     /locus_tag="LZB08_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBM7315405.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1469..1942
                     /locus_tag="LZB08_001902"
     CDS             1469..1942
                     /locus_tag="LZB08_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1601..1603,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBM7315406.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            1974..3284
                     /gene="grdB"
                     /locus_tag="LZB08_001903"
     CDS             1974..3284
                     /gene="grdB"
                     /locus_tag="LZB08_001903"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3021..3023,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBM7315407.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3541..5073
                     /locus_tag="LZB08_001904"
     CDS             3541..5073
                     /locus_tag="LZB08_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBM7315408.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5084..6229
                     /locus_tag="LZB08_001905"
     CDS             5084..6229
                     /locus_tag="LZB08_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBM7315409.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6543..7622
                     /locus_tag="LZB08_001906"
     CDS             6543..7622
                     /locus_tag="LZB08_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBM7315410.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVEILRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7735..8334
                     /locus_tag="LZB08_001907"
     CDS             7735..8334
                     /locus_tag="LZB08_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315411.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8521..9393)
                     /locus_tag="LZB08_001908"
     CDS             complement(8521..9393)
                     /locus_tag="LZB08_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7315412.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9810..11213
                     /locus_tag="LZB08_001909"
     CDS             9810..11213
                     /locus_tag="LZB08_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315413.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11240..12784
                     /locus_tag="LZB08_001910"
     CDS             11240..12784
                     /locus_tag="LZB08_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBM7315414.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12815..14206
                     /locus_tag="LZB08_001911"
     CDS             12815..14206
                     /locus_tag="LZB08_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBM7315415.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIVKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14399..15895
                     /gene="abfD"
                     /locus_tag="LZB08_001912"
     CDS             14399..15895
                     /gene="abfD"
                     /locus_tag="LZB08_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBM7315416.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16030..16305
                     /locus_tag="LZB08_001913"
     CDS             16030..16305
                     /locus_tag="LZB08_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315417.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16329..17636
                     /locus_tag="LZB08_001914"
     CDS             16329..17636
                     /locus_tag="LZB08_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBM7315418.1"
                     /translation="MSWQELYQSKLCSAIEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLIDIAHPSVRESLAVEFEKRFKEKY"
     gene            17656..18774
                     /locus_tag="LZB08_001915"
     CDS             17656..18774
                     /locus_tag="LZB08_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBM7315419.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         7227 a         2150 c         3738 g         5755 t
ORIGIN      
        1 ttgtaaaatg atataatttt gagaaaaact caaatgcata tacacaaatt aaataacagg
       61 aggtgcaagt atgcgtcttg aattagggaa aatctttata aaagatattc aatttggtga
      121 tgtaacagaa gtaaaagatg gagtattatt catcaataag caagagatgc tacaagaaat
      181 tggtggagat gagcatatta aatctataga tatagaatta gctcgtccag gtgaaagcgt
      241 aagaataaca cctgtaaaag atgttattga gccaagagta aaagttgaag gtaatggggg
      301 aatcttccct ggtatcatgt ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt
      361 aaaaggtgta gctgttgtaa ctactggaaa aatagtaggt ttccaagaag gtataataga
      421 catgactgga gaaggagcta agtacactcc attttctaaa ttaaataatg tagtagtaat
      481 agctgaacct attgatggtt taaaacaata cgctcatgaa aaagctgtta gaatgatagg
      541 attcaaagca gcaatgtact taggagaagt agcaagaaat cttactccag atgaagtttc
      601 agtatatgaa actaaacctc ttcttgaatc aataaaagaa tatccagagc ttccaaaagt
      661 aggatatgtt tacatgcttc agactcaagg tttattacat gatacttatg tatatggagt
      721 agatgcaaaa caaatagtac caactttatt atatccaaca gaattaatgg acggagcaat
      781 tgtaagtggt aactgtgttt ctgcatgtga caaaaaccca agttatgttc atataaacaa
      841 tggggttgtt gaagacttat atgcaagaca tggtaaagat ataaactttg taggtgtaat
      901 tataactaat gaaaatgttt atctagcaga taaagaaaga tcttcaaact ggacttctaa
      961 attatgtaaa tatttaggat tagatgcagt tatagtttca caagaaggtt ttggaaatcc
     1021 tgatactgac cttataatga actgtaagaa gatagaaatg caaggtgtta aaacagttat
     1081 agttacagat gaatatgctg gacgtgatgg aggttctcaa tcacttgcag atgcagatgt
     1141 aagagctgat gctgttgtaa caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt
     1201 agataaagtt attggacatt tagaagtagt agatgtaata gctggtggtt ctgatggaag
     1261 tttaagagct gatggaacta ttgaagttga aatccaagct ataacaggtg caactaatga
     1321 aacaggattt ggtcatttaa ctgctaaagg atattaagaa gtattttatt aaacgattga
     1381 gtaatgttaa taaaacatat tatagataat tataataagt gttatagata tataaaaata
     1441 tattagaaat ataataagga ggattattat gagtttactt agtaataaaa aggttcttat
     1501 aataggtgac cgtgatggta taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga
     1561 aggagcagag gttgttttct catctacaga atgctttgtc tgaacagctg ctggggctat
     1621 ggacttagaa aatcaaaaca gagttaaaga tgctgctgat aaattcggag ctgaaaatgt
     1681 tgtgatttta ctaggtgctg ctgaagccga agctgcaggt cttgcagccg aaacagtaac
     1741 tgctggagat ccaactttcg ctggaccact tgctggagtt gccttaggat taagtgttta
     1801 ccacgttgtt gaggaaccaa taaaatcatt atttgatgaa agtgtatatg aagaccaaat
     1861 aagtatgatg gaaatggttt tagaagttga agaaatagaa gaagaaatgt ctggtataag
     1921 agaagaattt tgtaaatttt aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa
     1981 aactaaaagc agtacattat attaatcagt tctttgctgg tataggtgga gaagaaaaag
     2041 ctgatactaa gccacatgtg gcagaaactt tacctccaat aagtttacaa cttgataaat
     2101 tattaggtga agatattgaa atagttggaa cagtagtttg tggagatagc tatttcaatg
     2161 aaaatataga tagtgcaagt gaagaagttt tatcaatggt aaaaggtttt gaaccacaac
     2221 ttttcatagc tggtcctgca tttaatgctg gtagatatgg ggttgctgct ggaacaataa
     2281 ctaaagttgt aaaagatgct ttgaatatac cagctttaac aggtatgtat gttgaaaatc
     2341 caggagcaga tatgttcaaa aaggatgttt atgtagtaga aacttctgat tctgcagcag
     2401 gtatgagaaa ggcacttcct aagatagcta aacttgcagt aaaacttgct aatggagaag
     2461 aaattggaac tcctaaagat gaaggataca tagctagagg aataagagtt aactacttcc
     2521 atgaagacag aggttctaag agagctgttg acatgttagt taagaaaata aaaggtgaac
     2581 catttgaaac tgaataccca atgccaaact ttgatagagt tgacccaagt aaagctgtaa
     2641 aagatttatc aaaatgtaaa atagctttag ttacttctgg tggtatagtt ccaaaaggaa
     2701 atccagatag aatagagtct tcttcagctt ctaaatatgg aacttattct atagcaggag
     2761 ttatggattt aacagaagag acttatgaaa ctgcacatgg tggatatgac ccagtgtatg
     2821 caaatttaga tgcagataga gtattacctg ttgatgttct tagagattta gaaaaagaag
     2881 gtgtaattgg aaaattacat gagactttct atacaactgt aggtaatggt acatctgttg
     2941 caaactctaa aaaatatgct tctgaaattg gagcagcatt agtggctgat ggtgtagatg
     3001 cagtaatatt aacttctaca tgaggtactt gtactcgttg cggtgcaacg atggtaaaag
     3061 aaatagaaaa aactggactt ccagtagttc atatgtgtac agtagtacct atttcattaa
     3121 cagtaggagc taatagaata gttccaacta tagcaatacc tcatccactt ggaaatccag
     3181 cgcttgaccc tacagaagaa aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg
     3241 ctttaactac agaagtagat ggtcaaacag tatttgaaaa ataaaaataa cacacacata
     3301 tattatatag ataaaaatat aaacacacac taaaaataaa aaataactat acacaaaaaa
     3361 aataatatag atttcatgaa tggataaatt ggagttattc attcatgaaa tcttaaaaaa
     3421 tgccaaaata cataaacaat aatagaatca aatatacaat aaaatacaat gattagagct
     3481 tttaataagt ttaataagat taaaaaataa gtgttcataa aatttatgga ggtgcacaaa
     3541 atgacttatc cagttttaaa aggggcaggc tatgtactta ttcatactcc agatatgata
     3601 gtgcaaaatg gaagtacttg tacagttgaa agagcaacaa atccagattc agaattttta
     3661 aaagaagtaa gtaatcatat aagaagttat gaagatgtag ttaactacat gccaaatcaa
     3721 gtttatatag gaaatagaag accagaagaa ttaagagatt taccaatgcc atggtgtgaa
     3781 caaaagattg agggaactag aaatggtaag tttggagaaa taatgcctca agatgagttc
     3841 atagctttaa tgcaaataag tgatgcattt gatttagtaa aattatcaca agaatttatt
     3901 gatgaagtaa agccaaaaat cgaaaataac taccctgaaa ttgcaccatt tgtaggtaaa
     3961 ttaaagggtg atgatataga agaaggtaaa gaattagtag caacacacat agctgaagga
     4021 ttataccatg atggaaaatt tgttggttat gtaaaaagag cacatgatgt agatgttaac
     4081 ttaaatgccc atacaatgtt tgaaaatcta gtagtaaaag catctggagt tttatctgct
     4141 attcaaatgc taagacatag caaaatagac cctgctgaaa tagattatgt tattgagtgt
     4201 tcagaagaag cttgtggaga tataaaccaa agaggtggag gaaactttgc taaatcaata
     4261 gctgagatag caggacttca aaatgcaact ggttctgaca caagaggatt ttgtgcagca
     4321 ccaactcatg cattaattca agcagcagct ttagtaaaag ctggcataca taaaaatgta
     4381 atggtagtag caggtggagc aagtgctaaa cttggaatga atgctaaaga ccatgttaaa
     4441 aaaggtcttc ctgtacttga agatgttgtt ggaggatttg cagtacttgt atctgaaaat
     4501 gatggtgtaa atccagttat aagaactgat ttaacaggta agcatactgt tggaactggt
     4561 tcttcaccac aagcagttat gacagcactt ataacttcag gtcttgatag agctaattta
     4621 aaaataacag atgttgatgt gtattcagtt gaaatgcaaa acccagatat aactaagcca
     4681 gcaggagcag gagatgttcc agaagcaaac tacaaaatga taggggcact tgctgttaaa
     4741 cgtggagact tagaaaagaa agaattgaaa gattttgttt ctaacaaagg attaccagga
     4801 tgggcaccaa ctcaaggaca tataccatca ggagctcctt atataggatt tttaatagat
     4861 gatttaacta caggaaatag aaatagagct atgatagttg gaaaaggtag tttattctta
     4921 ggaagaatga ctaacttatt tgatggagta tcttttatag ctgaaagaaa tacaggtgtt
     4981 actgaagaga cttcaggaat atctaaggat gaaattaaga agataatagc tgaatctatg
     5041 aaaaaactag cattagatat gttagaagaa taggggtgag gatatgtcaa aaaaagtaat
     5101 cgcagatgta ttcttagaag tagctaatgc aatagaaagc ggcgaatttg gcaaaaaagt
     5161 aaaaattggt gttactacac ttggaagtga acatggtgta gaaaacatgg ttaatggtgc
     5221 acaattagca aaatctaatt tatttgatat tgtgcttatt ggaccaaaag tggaaactga
     5281 ccttgaagta gttgaagtta atgatgaaaa agaaatgcat gcaaaaatgg aagaattatt
     5341 agattcagga tatattgatg cttgtgttac tatgcattat aattttccaa taggtgtatc
     5401 tacagtaggt agagtaataa ctccagcaaa aggaaaagaa atgatacttg caacaacaac
     5461 aggaacaagt gctactaata gaatagaggc aatggtaaga aatgccattt atggtattgc
     5521 tacagctaaa tcaatgggta acaaatgccc aaaagttgga atacttaatg ttgatggagc
     5581 aagacaagtt gagaagtgct taaaagaatt aaaagataat ggatatgaca tggagtttgc
     5641 ggattcaatt agagcagatg gtggatgtgt aatgagaggt aatgatttac ttgtaggtgc
     5701 acctgatgtt atggttactg atacattatc tggaaatata tttatgaaag tattttcttc
     5761 atatactaca ggtggagatt atgaagctca aggctttgga tacggacctg gagttggaga
     5821 agactatgat agaaaggtac ttatagtatc aagagcatca ggttctccgg tagtagcaaa
     5881 tgctttaaaa tacgcttatg atgttgtaaa gggagatata agtaatgttg caagaaatga
     5941 atttgcaaag gttaaaaaag caaaatttga tgacattata tcctctctta ctaaaaaaga
     6001 agttaaagct gaaaaagtag aagttaaaat gccagataag gaaattgtta ctcgtcaaat
     6061 agctggtgtt gatataatgg atttagaaga tgcagtatct gagttatgga aaaacggaat
     6121 ctatgctgaa agtggtatgg gttgtacagg tccaatagtt ttagtaaatg aagctaaagg
     6181 agacttagca gttgaaactt tagttaaagc tggatatact gcaaaataag tacatttttt
     6241 atatgtaaat aagaacatat agttataatt ttattgatta taattatatg ttctttttat
     6301 tttatatctt gtcaatattt atatattaac acaatccatt tatttgattc tctagctaat
     6361 ttattatatt tatttaatta aaattaagct ttctaactaa ataaaaacta atgaagtatt
     6421 aaaaaaaatg aaattaataa aatatgaaaa aattttagga ataaagtatc tgttagataa
     6481 aaataagatt aattagtgat ataatataat taagataaaa tttattattt ggaggtaaaa
     6541 tcatgagcag agtaaaaaat gtagtagaat tgttggaaac taaaggtgtt gatgcactat
     6601 atctaacaaa gaaaactaat gtaaactata taagtggttt tcctgatgaa gaagcatatg
     6661 cagtgatttg caaagatgga aactttttag ttactgatag tagatatatg gagcttgcag
     6721 aaaaggtttg caaagatttt gaaattataa attggcataa ttttgataga agtgtggcaa
     6781 aggcagttaa aagtgtatgt gataaagtgg gtataaagaa acttgggttt gaaagaacta
     6841 atatagtgtt tgataaatat gaagaactta aaaatctgat tgagaaggat aatggagaat
     6901 taatacctac agaaaatatt gtagagattt tgagatatgt aaaagacaaa gatgaaatca
     6961 aaaatacaag aaaagcttgt gaaatagcgg ataaggcatt agaagaattg attccacata
     7021 taaaagctgg agttagcgaa attgaactgg ctacaaggtt agagtacttt atgaaaatga
     7081 acggagcaca aaatataggg tttgaaacta tactgatatc aggagcaaag acttcattgc
     7141 ttcatggtaa gcctagtgat aagattatag aaaaaggtga ttttgtactt atagactatg
     7201 gtgctatgta taatggctat atatctgata caactagaac ttttatagtt ggaggagctt
     7261 ctgaaaagca attagaaata tataatttag ttaaggaagc tcaaaatgta ggggttgaga
     7321 acatgaaagc tggtgtacat gcgacaattc cagatgctga gataagaaaa gttgtcaaaa
     7381 aatatgaaga ctattactat caaggtatag gacatggagt cggtagagat gttcatgaag
     7441 aacctttcat tggtaattat ggtgataaaa tcatagaaga gggatgtata ataacaatgg
     7501 aaccaggtat ttatttccct ggatggggtg gagttagaat agaagatact gttttgatta
     7561 ctaaaaatgg tccagaaaga cttactaaat tccctaaaga tttgatgatt ttagacaaat
     7621 aatttaattt atatatattt ttttacataa aatataacat aatctcacta ttaagctcta
     7681 tttacatatt atagtaaaga gggcttaatt ttattgtgag aggtgatagt atatatgtat
     7741 aaaaaacata aatttataaa aaatataaaa aaagcctggt ataaaatcaa attaattgat
     7801 agatgcctta taattgtaat ggcaatatta atgtttcaat ctatatataa tttatttgtt
     7861 aatgaagtaa attcacaaga tactacaact attgatgtag tagtaagaac cacttcagca
     7921 gctatatttg ggtatttttt aagtgctaat tttataaaga gacctttaag gaagtataca
     7981 aatactgatg tatcttcaaa tctttttata gataataatt tatccccaaa aggtcaagag
     8041 tctagtcaaa ataatataat gaatgtcaaa aataccattg gatttacttc agaagctgat
     8101 aattatgaaa agaaaatacc tattaataat aatgaaggtt ttgaagaagg agaaactagt
     8161 gaattgcaaa ttattatagc tactgtaatt tgtatagtag cattaacagt actttttgtt
     8221 gtaagaaatt ttacaactac aacatcagct tctttaggta ctatttcaca gatgagagat
     8281 tttgtctcag gatgcgtggg ttttttgcta ggttgtccaa gtaaacctga ttaaaaaagt
     8341 attatcaata aaaggtattt taaaaagtat cagtataata atatttagat ttaaatgtat
     8401 acaattataa ttctgtaaca ttctatagtg tatttttata ctgatactct aaactatact
     8461 atttattaaa atattttaac attgtattta aacaaaatac tatatctgta taatataaat
     8521 ttagtttata tgaatagatg ccatatactc aataaaaagt ttcatactat gagatatgta
     8581 ttgatttctt ctccaaagaa ccccaatatt tgcagtaatt gcacgttcta atggtattga
     8641 acagagattt tctgtatttt gaaatatttc tttatacaat attgttgaag ccaagtcatt
     8701 ttctagtaaa taacgtatgg tagaaagttg atttgagtgt aaaattatat ttggcttagc
     8761 tttttcaagt ctttttgtaa taatcttata gtgataagaa ccttcagata agattacaaa
     8821 aggtacattt tttaacatat aattcgatat aatgggatgt tttgccaatt catttttggg
     8881 atttgtaact aagtataatt ctgttttata gattgtcttt gaatctatat catcataaat
     8941 atcactgtca attataccaa tacaaaaatc aagtgtagat tcttcaatca ttttagctcc
     9001 aacaaatgtt ggaacttcaa aaattttaag ctctatatct ggataaatta cattaaatct
     9061 aggaagtatt tttgaaagca aaaacgtacc taaaactgta ggaataccta ttttgagaga
     9121 agcttttctc ttttttccta aatcaagtgc gtcacattga aaatcctcaa attgttttat
     9181 aaaatgtttt gacttgtcta aaaaaatttt tccttcaggc gttaactcta ttttattacc
     9241 tattctgtaa aaaagttcaa agccaagttc tttttctaat tctttaattg ctaatgttat
     9301 ggatggttga gaaatatgaa tactttccgc agccttagtc atgtttttat atttacagac
     9361 agtttgaaaa tagtataaat agtttatatt catttggtat tagttccttt catttacata
     9421 ttagtgacaa gtatataatt aacaatagca taagttttgt atattgttgt aaaaaaatag
     9481 aatagtttta aactatcata gcataagttt ttgatatttt caatctatta agaatagtgc
     9541 tattattaaa ttaacaaaag agaatagctg gtatggttta gagtaaatca atctatatta
     9601 cagttatatc aacgaatatc aaaaaattat ctattaaaaa ataaaaatta tttttacaat
     9661 taaattgtta attaaatcat aaagaaaaaa gtgatattaa caagataaaa tttttttaat
     9721 cagaaatgtt aatttaataa caaaatagag tattggggag aaaggagaaa tatatttttt
     9781 aataatattt gttaatttaa taacgaaaaa tgaaagggag tgatttagtg aagtttgaat
     9841 tgatggattt tttaatgaat ccttttgtcc ttatgtttgc tgcagtaatt acaggaattt
     9901 tatttggaaa aattaaattt gggaagttta attttggggt atcaggagca ttatttacag
     9961 gattatttat tggatggctt gcatatagct tggggaatct aataatagca aaaggggaaa
    10021 cagcagcagg atataaggca gcaactgtaa tgatggggaa tggaattatt tcttctgact
    10081 tctttgattt ctttttaata atatttgttg cagcagttgg tctattggca gcaaaagata
    10141 tgaaagctgt attaaagaag tatggagcaa gatttgtaat tttaggagtt cttataactt
    10201 ttataggtgg ctctatgact tatgcaatga ctttactatc aagtgataaa ggaagtagtg
    10261 cttatgaagt ttctggcgtt tacacaggag cattaacaag ttcgcctgga ttagctgctg
    10321 ctttagaaac agctggtaaa catgcagaag atgtatcaaa agaatttgaa aaagcatcaa
    10381 taaaagataa aaaagaaatt ttaaaagtag tcgacccaga aggcaaattg gatgtgaata
    10441 caactacatc actgactcaa gagcaaatag ataagtatat tgcatatgct gaagctggtg
    10501 ttggtatagg tcatgcagtc gcatatcctt ttggagtttt aatagtaatc ttaggtgtaa
    10561 atttccttcc aaaattattt aggatggacc taaaagaaga aagaagaaaa tatgagaagg
    10621 aaatgaagga agcaagagat agtgtttctg gtaaaacgat accagaagtt ccatttaata
    10681 taataacatt tttcttgaca tgtttagctg gatacttagt tggaggaata catgtattta
    10741 tgggaccact agggtacttc acgctaggtg caacaggagg ttcactaatt gtatctctag
    10801 ttcttggata tataggtaag ataggggtag taaatttcag aatggaagaa aaggttttaa
    10861 atattttaaa acaaataggg ctagtatttt tcttagcaat agttggatta agatatggtg
    10921 gaaaagtagt tgattcaatt atgacatctg gtatgcatct agccttagta gcaatagcag
    10981 taggtgttac agctatgatg ataggattct tagttggtaa atacgtattt aaattaaatt
    11041 ggattttatt atctggagct gtctgtggtg gtatgacatc aacaccaggt cttggagctg
    11101 ctgttgatgc tcttgatagt gatgacccag cagcaggtta tggtgctacg tatccatttg
    11161 cattattaac taaagttata ttggttatag ttttacacaa acttccaatg taaataagat
    11221 tttatatggg gggatattta tgaataatga aggagcattt gaagaattaa gtggaagatt
    11281 aagaaataaa gaattgtgtg atttaataat gaatgcagat gaagctgcta aaattataga
    11341 ggatggcatg gttattggag ttagtggatt tacaccttca ggctatccaa aagcagtacc
    11401 attagcagtt tcagaaagag ctaaaagtgg agaaaagata aaattaaccg tatattcagg
    11461 agcatcttta ggtccagaag ttgatggagc atggtcagaa gcaggtataa ttgaaagaag
    11521 acttccttat caaactaatt caatacttag aaataatatt aataaaggtg ttgttgatta
    11581 tatagatatg cacctaagtc attctactca atttttaaat tatggaacta tacctaaagt
    11641 agatgtagct atagtagaag cacttgcaat aactgaagaa gggaatatta taccaactag
    11701 tgggataggt aattctccat cttttataaa aagtgcagat aaagtaatag tagagataaa
    11761 tcttgcaaaa ccaatggaaa tggaaggtat ggcagatata tatattacgg aaaatccacc
    11821 aaatagaaaa ccaatagaaa taaatcatcc taaggataga attggaacaa catatatacc
    11881 ttgtggtttg gataaaattg caggagtagt aattaccaat atgcaagata agacccgtcc
    11941 attgggggtt gtagatgaag cttcgaaaaa aatatcaaat aatattattg cttttttaag
    12001 agaagaagta aaggcaggca gattatcaaa gaatttactt cctcttcaat ctggtgtagg
    12061 aagtgtagca aatgcagtat tatatggatt atgtgaatct gaatttgaaa atttaacttg
    12121 ttatacagag gttgtacagg actctatgtt ggatttaata agaatgggta aagttacaat
    12181 ggcatctact acatctgtaa gtccatctcc agaaggatta ataaaatttg aaaaagatat
    12241 tgattttttt aaggataaga ttatattaag acctcaagaa attagtaaca atccagaaat
    12301 agccagaaga attggtgtaa ttgcaatgaa cactgcaatt gaagttgata tttatggaaa
    12361 tgtaaattct actcacataa tggggtcaaa gatgatgaat ggaattggag gttctggtga
    12421 ttttgctaga aatggagcaa taactatatt tagtactgag tcaattgcaa aaaatggtga
    12481 tatatcatca atagtaccta tggtatctca cgtagaccac actgaacatg atgtaatggt
    12541 aattgtaaca gaacaaggat atgctgattt aagaggtctt gcacctagag aaagagctat
    12601 aaaaataata gaaaattgtg cccatccaga ttataaagaa caacttaggg attatttaaa
    12661 tagagcttgt aaaagtggag caaaacaaac acctcacata ttggacgaag ctttatcatg
    12721 gcatagtaaa tttatgagta caggtacaat gaaaaaggct gaaactttta aaagtgcatt
    12781 ataaaatatt taataaattg gaggtagatt ttaaatggaa aaagcagtgg aaaactttga
    12841 agatttaagt aaggaataca ttaatggtta tattgagaga gcaagaaaag ctcaaagaga
    12901 gtttgaatgt tatactcaag aacaagtaga taaaatagta aaaatagttg gaaaagtagt
    12961 gtattataat gctgaatatt tagctaaact tgctgtagaa gagactggaa tgggagtata
    13021 tgaagataag gtagctaaaa ataaaagcaa agcaaaagtt atatataaca atttaaaaga
    13081 taaaaaatca gttggtataa ttgatataga tagagaaact ggaataacaa aggtagctaa
    13141 accagttgga gtagtagcgg caataactcc ttgtacaaat ccaatcgtta cacctatgag
    13201 caatgcaatg tttgcactta agggaagaaa tgcaattatt atcacacctc atcataaagc
    13261 aattggatgt agtacaaaaa ctgtagagat gattaatgaa gaattagaaa aaataggagc
    13321 tccagaaaat ttaatacaaa tacttgacca acaatcaaga gaaaatacaa gaaatttaat
    13381 ttcatcagca gatgtagtaa ttgccactgg aggaatggga atggtaaaag ctgcgtactc
    13441 aagtggaaaa ccagcacttg gtgtaggggc aggaaatgtt caatgtatca tagatagaga
    13501 tgtagacatt aaagaagcag tacctaaaat catagcagga agaatttttg ataatggtat
    13561 catttgttca ggtgaacagt cagtaatagt agcagaagaa atgtttgata aaataatgga
    13621 tgaatttaaa aataacaaag gttttatagt tagagataaa gtacaaaaag aagctttcag
    13681 aaatgctatg tttgtaaata aatctatgaa taaagatgca gtaggtcaat cagttcatac
    13741 aattgcaaaa attgcaggtg ttgagatacc agaagataca aaaataatag taatagaagc
    13801 tgatggacca ggagaagagg atattattgt aaaagaaaaa atgtgtccag ttatatctgc
    13861 atacaagtat aaaagttttg aagaaggtgt agctattgca aaagcaaatt taaatgtaga
    13921 aggaaaagga catagtgtat ctatacattc aaatacagtt aaaaatatag aatatgcagg
    13981 agaaaatata gaagtatcaa gatttgttat taatcaatgt tgtgctacta gtgctggagg
    14041 aagtttcttt aatggtcttg cacctactaa tactctaggt tgtggttctt ggggaaataa
    14101 cagcatctct gaaaatcttg attataagca tctaattaat atttctagaa ttgcttatta
    14161 tatgccagaa aatgaagtcc ctacagatga agaactttgg ggataaaatt tagacaaaaa
    14221 atattcattc taaattaaag taggaatata ttttaattta gaatgaattt aagacataaa
    14281 ttaaatttaa attagtttat tggactaaat ttaaaatatt aagagacata tagaagaagg
    14341 aagtattata atttaaaata aataaaaaaa tctataaaag agaaggagtg gttttattat
    14401 ggcattaatg acaggagctc aatatattga aagtttgaga aaattaaata ctaaggttta
    14461 tatgtttggt gaagaggtta agaattgggt tgaccatcct atgattagac catctataaa
    14521 ttgtgtagca gcaacttatg acttagctca tgacccagaa tatgcagatt taatgacagt
    14581 tacttcaaat atcacaggtg aaaaaataaa tagatttggt catcttcatc aaagtgttga
    14641 tgatttgata aaaaaagtaa aaatgcaaag actatgtggt caaaaaacag cttcttgttt
    14701 ccaaagatgt gttggtatgg atgcgtttaa tgcagtgtat tcaacaacat ttgagtgtga
    14761 taaagcccat ggaactaatt atcatgataa ttttgtaaaa tacttaactt atatacaaga
    14821 aaacgattta gtagttgatg gagctatgac tgaccctaag ggagatagaa gtctatcacc
    14881 tagtgctcaa cctgacccag atatgttcct tcatatagta gaaagaagag aagatgggat
    14941 aattgtaaga ggagcaaagg cacatcaaac tggttcaata aattcacatg agcaccttat
    15001 aatgcctact ataagtatga ctgaagcaga taaagactac gctgtatcat ttgcagttcc
    15061 ttcagatgct gaaggagtgt ttatgattta tggaagacaa tcttgtgaca ctagaaaatt
    15121 agaagaaggt gcagatgttg atttaggaaa taaggagttt ggaggtcaag aagctttagt
    15181 agtatttgat aatgtatttg ttccaaatga tagaattttc ttatgtggag aatgggattt
    15241 ctctggtatg ttagtagaga gatttgcagg ataccataga caaagttatg gtggatgtaa
    15301 agtaggagta ggtgatgtaa taataggagc agcagcttta gcagctgatt ataatggagc
    15361 aaacaaagct tcacatataa aagataaact aatagaaatg actcacctta atgaaagctt
    15421 atattgctgt ggtatagcat gttcatcaga aggtcacaaa acagaagctg gaaactatca
    15481 aatagattta cttcttgcaa atgtgtgtaa acaaaatgta actagatttc cttatgaaat
    15541 agtaagactt gcagaagata tagctggagg gcttatggtt actatgcctt cagaaaaaga
    15601 ctttaaatca gaccttaaag ttggaacaag tggaatgact attggcgaag tatgtaacaa
    15661 gtattttaaa gcttcttcag tggcttcaac agaagaaaga atgagaatat taagattctt
    15721 agaaaatata tgccttggtt catctgctgt tggttataga actgagtcaa tgcatggggc
    15781 aggttcacca caagctcaaa gaataatgat ttcaagacaa ggaaacataa atcaaaagaa
    15841 agaattagct aagaaaattg cgggaataaa aaaagaggaa gctttaaatt tataaaaagt
    15901 tgatatataa agggaaatta tatattaatg tgtattaatt aatgaaaaaa tatgtagtat
    15961 aagagatagt tataaaatta tggtggtttt tagctaagta aagaaatttt taataaggag
    16021 aatttacaca tgacctgtgg agttagattt tgtgggggct gtaaccctag atacgaaaga
    16081 gggaaggctc ttacaataat caaagagcac ttcaatggta gggtagattt tagtatagca
    16141 caagagaacg tcgagtatga tttgctactt ataattggag gatgcgcaac tctatgcgca
    16201 tcctatcaac aatataaagt gaaaaatgaa tgtgttaaga tgtgggattt tagccatata
    16261 gaaagagtta taaatgtgat tgaatcagca atttgtgaaa cttaaaaaag ctaaatgggg
    16321 ggatttcatt gagttggcaa gaattgtatc aaagtaaatt atgtagtgct atagaagctg
    16381 taaaacagat taaaaacggt gatactgtag tatttgcaca ttgtgtaggt gaacctcctg
    16441 cactagtaga ggctatgatt gaaaatgctg aacaatataa agatgttgag attaaacata
    16501 tggtaagctt gggtagtggt ggatatactg ctaagggaat ggaagctcat tttagagtaa
    16561 atccaatgtt tgtaagtgga aatgtaagaa aggcaattga aaatggagat ggtgatttta
    16621 cacctgcatt cttccatgaa gtaccaaagt tgcttagaga aaaaagatta aaatgtgatg
    16681 ttgttttagc acaggtaacc ccaccagatg aacatgggta ttgtagctta ggaacatcag
    16741 ttgattatac ttatgaagcc attaaaactg ctagaacagt aattgttcaa gtgaatgacc
    16801 agtttcctag aacctatggt gaggtagtcc atgtatcaga gtttgactat atagtggaaa
    16861 aatcacaacc tttatttgaa ctacaacctg caaagattgg agaagtagaa gaagcaattg
    16921 gtaaaaattg tgcttcatta attgaagatg gttctacttt acagctaggg attggtggga
    16981 taccagatgc agttatgtta tttttaactg ataaaaagga tttagggatt cattcagaaa
    17041 tgatttcaga tgggactttg gccctttatg aaaaaggtgt tataaatggt aaatataaaa
    17101 attttgataa agaaaaaatg acagttacct tcttaatggg tactaaaaaa ttatatgact
    17161 ttgcaaataa taaccctgca gtagaggtaa aaccagtaga ctatgtgaat catccagcaa
    17221 ttataatgaa acaacataag atggtttcta taaattctgc tatacaagtt gatttaatgg
    17281 gacaggtagt tgcagaggct atgggactta gacaatttag tggtgttgga ggtcaagttg
    17341 actttatacg tggagtgtct atgggtgaag atggaaaggc aattatagct atgccttcaa
    17401 taactacaaa aaaagatggt acagtaattt caaaaatcgt atctattgtg gatgaaggtg
    17461 cacctataac gacatcaaga aatgatgttg actatattgt aacagaatac ggtatagcag
    17521 aattaaaagg aaaatctctt agagaaagag caagaaattt aatagatata gctcatccaa
    17581 gtgtaagaga aagtctagca gtagaatttg aaaagagatt taaggagaaa tattaataat
    17641 agaggggtgt taataatgga agcacttaga gtagttccaa agatatttta ctttgataca
    17701 tttaaagaat ttaatgaaga atttaaaata ggaaaaaatg atttagtaat tactaatgag
    17761 tttatatatg agccatatat gaaacctctt ggaatagata caaatttaat ttttcaagaa
    17821 aagtttggaa caggtgaacc atctgatgaa atgatagact ccatgacaaa ggaaatgaag
    17881 aaatacaatt ttgatagaat tattgcattt ggaggaggaa caatagttga tatatgtaag
    17941 atattagcac ttgatgttcc agaaaagtct attgatttat ttgaaggaga agtatcacct
    18001 aaaaaagtaa aagagttagt agtagttcct acaacatgtg gtacaggtag tgaagtaaca
    18061 aatgtagcta ttgcagagtt aaaatctaaa catactaaaa aggggcttgc agtagaagaa
    18121 acatatgcag actatgcagt gttaattcca gagacagtaa aagggttacc ttacaaattc
    18181 tttgttacaa gttcggtaga tgctcttata catgctattg agtcttatct ttctccaaaa
    18241 gcaagtccat ttacagagat gtattctcta caagcaataa aaatgattat ggatgggtat
    18301 aagaagatag ttgataaagg agaagaagag agatttaatc atcttagaga ctttgtactt
    18361 gcatcaaatt atgcaggaat tgcttttgga aatgctggtt gtgcagcagt tcatgcacta
    18421 tcttattcaa taggtggagc tttccatgtt gcacatggtg aagcaaatta tcagttcttt
    18481 actgaagtat ttaagatgta ttctagaaaa aaacctaatg gaaaaattat aaaatgtaca
    18541 aaaattttag cagatgccct tgaatgtgac cctaactgtg atgtttatgg agaattagaa
    18601 aagtttttaa ataaattaat agctaaaaaa gctttgagag aatatggaat ggttgaaagt
    18661 cagatagatg aatttacaga tagtacaata gcaaatcaac aacgactact tgctaataat
    18721 tatgtagagc ttagtagaga ggaaataaga gagatatttg caaatcttta ttaatacaat
    18781 tttttattta tatgtatagt caacataaac aatgctaatt atatttatgt acaaataaaa
    18841 ataagggaac ttaaatttta attaaagtta
//
LOCUS       DAFKMX010000071        18669 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFKMX010000071 DAFKMX010000000
VERSION     DAFKMX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.071"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..591)
                     /locus_tag="LZB08_001916"
     CDS             complement(<1..591)
                     /locus_tag="LZB08_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7315420.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(975..2189)
                     /gene="dpaL"
                     /locus_tag="LZB08_001917"
     CDS             complement(975..2189)
                     /gene="dpaL"
                     /locus_tag="LZB08_001917"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBM7315421.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2812..3351)
                     /locus_tag="LZB08_001918"
     CDS             complement(2812..3351)
                     /locus_tag="LZB08_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBM7315422.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(3428..5233)
                     /locus_tag="LZB08_001919"
     CDS             complement(3428..5233)
                     /locus_tag="LZB08_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315423.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5629..6006)
                     /locus_tag="LZB08_001920"
     CDS             complement(5629..6006)
                     /locus_tag="LZB08_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBM7315424.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVRAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6911..7396
                     /locus_tag="LZB08_001921"
     CDS             6911..7396
                     /locus_tag="LZB08_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315425.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7586..8125)
                     /locus_tag="LZB08_001922"
     CDS             complement(7586..8125)
                     /locus_tag="LZB08_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7315426.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8118..8531)
                     /locus_tag="LZB08_001923"
     CDS             complement(8118..8531)
                     /locus_tag="LZB08_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7315427.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKNMEAVSYE"
     gene            complement(8567..9688)
                     /locus_tag="LZB08_001924"
     CDS             complement(8567..9688)
                     /locus_tag="LZB08_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBM7315428.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLEMGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQENIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFVASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10287..11939
                     /locus_tag="LZB08_001925"
     CDS             10287..11939
                     /locus_tag="LZB08_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBM7315429.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKSELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12238..12987
                     /locus_tag="LZB08_001926"
     CDS             12238..12987
                     /locus_tag="LZB08_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315430.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            13000..15027
                     /locus_tag="LZB08_001927"
     CDS             13000..15027
                     /locus_tag="LZB08_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315431.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            15171..16754
                     /locus_tag="LZB08_001928"
     CDS             15171..16754
                     /locus_tag="LZB08_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7315432.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKDI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16726..17346
                     /locus_tag="LZB08_001929"
     CDS             16726..17346
                     /locus_tag="LZB08_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315433.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17535..18461)
                     /locus_tag="LZB08_001930"
     CDS             complement(17535..18461)
                     /locus_tag="LZB08_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBM7315434.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6396 a         2922 c         2238 g         7113 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaagtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 ctttccatca actatttttc cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgac cgtcaaataa tattttctta cctggcttgt taccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt ttcatttatt tagatttttc atttgcttta cttttttatt
      841 tgctttactt tttcatttat ttagattttt catttgtttt agtttttatt ttatttaaag
      901 ttttcactta tttaagtttt tattttcctt aaatttttca tttatttgtt aagtttttct
      961 tttccttaag tcttttattt actttggcat tcaccattcc aaactatatc tctatattta
     1021 tcagggtcag tatctccttc tgtacttatt aataatactt tagagttctc atctagtttc
     1081 aaagcttctc ttaagtcttt taaatcttca ctttcaagga ttgaaactaa tgctccagtt
     1141 gtaactgctc cagattctcc agagattact ttctcatctc ctcttagagg gtttcctaat
     1201 attctcatac ctttagcaga tacccagtct ggtgctgaaa cgaatgctga tgaataattt
     1261 tttaacactt caaaacctat agtgtttggt tctccacaag caagccctgc cattatagtt
     1321 ggcatatctc ctccaacaaa tcttggtttt ccatctccag cttctgctga tttatagtaa
     1381 caatcagcct catcagcctc tactactact gttattgggc attcatcata tattgaagca
     1441 acatatcctt gaactgcacc agcaagtgaa ccaactcctg cttgaacaaa tacgtgtgtt
     1501 ggtctatcta ctccatattc ttttaattgt tctatagctt ctttagccat agttccatat
     1561 ccttgcatta tccatgctgg tatttcttca tatccgtccc atgctgtatc ttgaaccatt
     1621 actccatggt catcagcttc tgcataatca ttagctaatc ttactgcatc atcatagttt
     1681 aagtctgtta tactagcatc tgcaccttct cctttgattt tatttaatct aaattcagaa
     1741 gaaccttttg gcattaatac aacagatttt tgacctaatt tattagctgt ccaagcaact
     1801 cctctaccgt gatttccatc tgtagctgta aagaaagtta tttctcctaa tttttctttt
     1861 atttctttcg aagtcaattt gtcataccct aactcagata tatctgtatt taatctttga
     1921 gctaagtatc tacccataga gtaagaacca cctaaaactt taaatgcgtt taagccaaat
     1981 ctataagatt catctttaac atatattccg cttacgccta gtgattttgc taagttatct
     2041 aatttaacta aaggagtctt ttcatattgt ggaaagctct catgaaaagc tttagccttt
     2101 gcaatctctt catcttttaa aaattcaatt cctttttctt tttcctctga tttaggtaag
     2161 ttatttaatt tccattttat ttctctcatt tattttatat tcccctttca aatttttcgg
     2221 tgtcatttta attttttatc tttgtgcctt atagcaaagc aattaccgtg ccaatattat
     2281 ttattctaaa aacaattttt ttatgttgga attacatact tttataaaat ttctttaaag
     2341 tttataaatt ttgagtgtga aaaataagat ttataaaaga aaaaattctc atattgggaa
     2401 atttttatca atacgagaat tatcaattcg agaaatcgtt tatttatctt aattcttcct
     2461 ttattatgct cttatttttt atatgtctat tgttttgtca gatatattac tacttttatt
     2521 ttctataaat atatggctat aaccttctta cgcataaact ctataaattt atatacttta
     2581 tattctttat tacccaaata ttctttatta cccaaagcct ccaaaatggc ttaaatcttt
     2641 tcatagcttc ttacagaagt ttacatggtc gtccccccaa taagcaaaag tgcaatatgt
     2701 gtaccattgt gccctacttg cgactattga gctgtcgaaa ttcgcaactt cgcatttatc
     2761 tgatgaaatg cactatacaa aatattcata acatcaacaa cagagttaaa gttacctatt
     2821 catattcact gtaaaaattt cttccacagg aaatgtaatt ttaaccaatc ctattgagac
     2881 aaactcacca cttatttttt tgaactcacg cagtaataca tcacttgccc gaaaaaaagc
     2941 ctcactttca tcatctccag taagtgctat tgtgcaatgg ggtacccaac gattggggtg
     3001 gtaccattcc caaccatttg aatcaaaatc ttgtagtacc tcatataatt ctctctgaaa
     3061 ctgatacata ctgctattca ttataggtga tacaaacacc gttttagtat cattaaacat
     3121 gcctactgaa ccaatgtaag caggtattac cttatggttt tgtgcaaacc cctttagctt
     3181 gtctatgcaa caagcctcat ccacatcatt gaaacaagcc aaagtaagat gaggtcttgt
     3241 tttccattct aagtacttcg tactaatttt ttcatcagcg atttttttag ataaatcaaa
     3301 aagttgcttt tccgtttttt tatcataata tagctctatc gcatattgca taataacacc
     3361 tccaccataa agtaaaaata cctatctata aaaacacaca aatacctatc attttaatat
     3421 tatattacta ctgttcctct tccaattttc tatatagtgt agccaaacct atgcctaagt
     3481 cttttgctat cttcttcttc gtctttgtat ctcttccata cttattcaac aacttgttta
     3541 tgtaagtcaa ctctaattct ttaattgaaa gtatcctttc ttgattctct actatccctc
     3601 caactatatc atcttcaaat attatattta catccttatt tttataaata tttccattaa
     3661 tattataata gtttaaaata tctaatggaa gcatatcttt atatattatt ccatcatctc
     3721 caaccaaatt catcatatat tcaactgcat tttccagttc ccttatattt cccggccaat
     3781 tatagttaat caacatattc attacatttt cttctatttt atttaactga atacctaatt
     3841 ctaaagaata tttagaagca aacttcatca ttattgcctc aatatcatcc tttctttccc
     3901 taagaggtgg caaatttata ggaattacat tcaatctata gtataaatca tccctaaact
     3961 taccttcatt tactaatttc aataagtttt tatttgtagc agcaatcact cttatatcta
     4021 aatctataag tttatttgac ccaattctcg caaattttct ctcttgtaaa actctaagta
     4081 actttgcttg taaaggcatt gacaaatctc ctatttcatc aagaaatata acaccttcat
     4141 ttgcaagttc aaatttacct actttccctc cactactagc tccactaaat gcacctttta
     4201 tatagccaaa tagctcactt tcaaggagat tttctggaat agcagcacag tttatcgcaa
     4261 taaatggctt gttccatctg ctaccttcag catgtatagc cctagcaatt agctctttac
     4321 ccgtaccact ttccccagtt ataagtacag ttgaatttga ctttgctaat ttcttagttc
     4381 tctcttttac ttttaacatt gcttctgaat ttccaattat agactctata tcactatttc
     4441 cccagccact attcactttt ccatgtcctt tatgattgat atatgtcttg tcaaatatta
     4501 ttattttgtc gtattgattt atatatggat ataccggaat tattttggca tttatattat
     4561 aaattttgtt atcaatatca atttctagta attcatgtcc atttgaggaa gattcaacag
     4621 atactatatt tactatattt tctattatat ttttatctat atctaatttt tttagagcta
     4681 tattgtttat ataagatatt ttgctattta tgtctagtat tataacgcct ttttcaacat
     4741 tttcaatgat ttctatcatc atatccattt ttctactgct attttttgac gcttcatgtt
     4801 cttctatttt catagaaatt aaatcgcata tatgattgac aaattttaaa taattatcta
     4861 tttttgcact tatttctact tttttagttt tatctgtact aatagcacca attactccta
     4921 tagttcgatt attatacttt attggaactg ctattattac tttatttaag catttcatgt
     4981 aatgggaaca ttctatacaa agctgattat cacatatatc caatacgact aattcctgac
     5041 ctgtctgtat aacattgtca taaataaatc cttctgtaac aacgctttca tttatctttg
     5101 atttatacag acctgtacca ctaattctta ttaatctatc gtcaactatt tcaatatcta
     5161 catctaacac actagctact gtttcagtgt attttatcat ttcttgttga aattctttta
     5221 atttacttaa catagtttcc cccattagaa tttaatttac caaattatca gtttagctat
     5281 attttaacat atagtcactc tttaaaatat atataaaaca ttacttattt atatatttgc
     5341 gttaaaacag acaatattaa tttataaaaa gtttgtttta tatagtaacc ttaattataa
     5401 tatgcagaca ataaaataaa tcacaataca ataataaaaa aaagctttcc tcatataaca
     5461 aatatatttt tgacataaaa atatacatta tatgagaaaa gctcaatagt taaatattta
     5521 gatagatttt tatatagact tttttaatat aaactttttt aattgtagac tttttttatt
     5581 agtttttaat ttttattagt tttgttattg gttttattgt tataatactt ataatactgc
     5641 cattgcttca atttctaaaa ctgcatcttt tggtaattga gcaacttgta cgcatgacct
     5701 tgctggtgga ttttctttaa aatatgtagc atatacttca ttaatatcat taaaatcctt
     5761 taaatctttt acgaatactg tagtcttaaa tattttatcc atacttgtac cagcttgttc
     5821 taatattgct cttacattat ctaaagaagc ttttgttgct tcttttgggt cagatactaa
     5881 ctcgccagtc ttcatatcta ttggaagttg acctgatgta gaaactatgt ttccaacttg
     5941 aattgcttga gaatatggtc caattgctcc tggagctttc tctgtagcta ttactacttt
     6001 tgacatgact tcacctccta ttattaagtt atatttacag gaaaataaaa tttcctaaac
     6061 accaatatta atacgaacct ttagattaaa tttcaaatct aaaacttcaa ataatcaata
     6121 cttagatgat tatgtttatt tgtgtcttgt taacttttat taaagcaatt aacgtgccaa
     6181 actttattta tatataaaat tttgtgaagt cattcaaaat agttacttct gaattttttc
     6241 gtaaaaaaac gctctaaaat tactttttta tgatgtcaat ttttttctca tatctagaaa
     6301 tttaccaaca tgagaattct tgttttgaga atttacagat taaagttatt ttttaacctt
     6361 aaattctgaa cctcccatga ctaaagtcac agggttcctg cttcatagaa ttttgcataa
     6421 tcaagttatt acatatgaaa gaactatagt tgacaatccc actattataa ataataaaac
     6481 ctttacccat accttattta ccctagttat catagagcct attgaaaata taacagctaa
     6541 atataataat aaccaaaaaa ttatcaatat ctaccactgt tgctgaaaat ccaaaaaact
     6601 accttttgat agtattactt aacaatctaa ttaatctcat aataaattta caacttataa
     6661 ataaattgtt atattggccg tatctcaaca actttctcct ttcctagttt taaatctttt
     6721 atagaattgt accacgtcta agtaaatttt catactatta agtcttattt ctaataaatc
     6781 attagctctc attctatgcc caataatgta agattgcagt aattttattg attttaatag
     6841 ttttatttaa tatacttata ttaattattt tcatatgaaa ttattattta tataggggga
     6901 tttttttaat atgagcaaaa gtgagttaac agcagaaaca acagaagaaa tgttagaagt
     6961 actaagtggt aaagattatg atattgcatg tcatttacat gaacttggta aatcattaga
     7021 ttgtaaaatt gaaccaaaaa caggtgctcg ttcttacaaa atagtatatt caactaagaa
     7081 accaaaacgt agcttattta ctattgaatg taatgaaaaa aaatggagag ttaaagcaaa
     7141 tctttttcat ctaaatacat ataaagatgc tgtggaggaa tgctctaaaa ctattaaaga
     7201 tagtataact aaaactcgta cttgtaagaa atgtaattca aagtgtattg gaggttcttc
     7261 gtttgaatta gatggaaagt cttacctgac ttgcatagga agtggtcatt attttgcaaa
     7321 tatggaagaa atggattgga aaaacctaga aaaattaatt actaaagaaa ataatattat
     7381 gcaggaatct gtatagtttt ttaagggtaa ttattaaaat atatgagtaa caacagatac
     7441 ttaaataggc ataaaaaaat aaagggtata ataaggatat agaataaaaa taaatgatat
     7501 agcacaaaga ggctgtctca aaacacagat aagcctcttt ttattgttga aatgataaga
     7561 atatattcaa ttttatacaa acatactacg ccaacttacc catttcatat gcctgtttca
     7621 ttgccttact tcccttaata tcacctacat tccaagcacc tacaccatat actatacctt
     7681 tttccttaga gccatttaga catgatgtaa atcctctaaa tccttctatt gttctttcca
     7741 tatcagccac attttcatct gctgctgtaa cgataaaata gaaatcttta tcacaaattt
     7801 cagtatatct agagcaagtt ctatcaatca tagttttcat ttgtgcattc atagtataaa
     7861 aataaactgg agtcgctaaa acaatcacat ctgccccaat cattttttca agaatttctc
     7921 ccatgtcatc tgtctgtgaa caatttgctt ctttttcata acaagcacca catccagtac
     7981 aatagttaat atctttatct tttaaaaata tcttctccac ttgattttta gattctaata
     8041 ccccttgtat aaactggtca cacaataaat cagaatttcc accttttcta ggactagctg
     8101 atactattaa tacattttta ctcataagat actgcctcca tattcttatt tctactgctc
     8161 tttaatactt tttctttctc aataatacct tcttcatatc tatcaatctt aaaatttaat
     8221 cgttccaaag ttttttgcat ttcttctatt ctttttgcta gttggtttct ctgctctatt
     8281 aaaagttctt ttctagcttt gatagtactg ctcccttgtt gaaacatagc tacatactca
     8341 attaatgtct caatagaaag acctgcacct ctcatacact ttatgaaatt aacccactca
     8401 caatcttctt ctgtaaaaga cctaattcca cttttatttc tatttacagg tggtattaaa
     8461 ccaattcgtt cataatatcg taaagtatcc gctgaaagct cgtatttttt actaacttct
     8521 gaaatcgtca tacatatctt ctcctcttga tattgtatag attgaattat ttctcaaagg
     8581 tttctgctac attctttaaa gcatctataa ttttaatgtg ttgaggacag ctttcttcac
     8641 attgtttaca ttcaatacaa tctgaggcct ttccataagt tttagtataa ttattatagt
     8701 agaccatctg tgttgaaaat ccagtattaa gagcttgttt ttctgcattg tacaacgcaa
     8761 aatagtttgg aatagcgata ttttttggac agccttctac acaatactga caggcagtac
     8821 aaggaactaa aattgattcg ttgataatct ctactgcttc atctataata ttatattcct
     8881 cttgtacgaa tggtttgaaa ttttgcatat atcctgtatt gtctaataat tgttccatat
     8941 ttgacatccc acttaagacc atcataacat tatctttact tgctacaaaa cgtattgccc
     9001 aagatgaaat tgacatatct ggattgtagt cattcaataa tttctctgct ttttctggta
     9061 tctttgcaag tgtacctcct tttactggct ccataacaat tacaggctta ttatgcttct
     9121 ctgccacctc gtagcatttt ctagattgta tgctttcatt atcccaatct aaataattaa
     9181 tctgtaattg cacgaagtct acttctggat gctcactcaa gactttatcc aataattctg
     9241 cactatcatg aaaagaaaaa ccaatattct ttatttttcc ttcttttttc ttttcttcaa
     9301 taaacttaaa gctatcaaat tttttagcta cttcataatg tgacacgcct atattgtgta
     9361 aaagatagta gtcaaaatag tctacaccac atttttctaa ttgctcatta aaaatgtttt
     9421 cttgctcttc ttttttcttt aaagccatta atggaagttt tgtagctata gtaaaactct
     9481 ctcttttgtg tctttttact agagattctc taagtgctat ctcacttttt cccatgtgat
     9541 acacataggc tgtatcaaaa tatgtaaatc ccatttctaa aaaagtgtct accattttat
     9601 ttaaatgttc catatcaata gttgtaacat cattttcatc ttttattggt aaacgcatta
     9661 atccaaatcc taattttttt gcctccattg ttttacttcc ccctttaaaa cttgtttgtt
     9721 ttttctctct actttaacta ttcttacaag tatataattc ttgtataatt atactttact
     9781 acttggagtt aacttcaagt caatagattt ttttattttt aagacaaata ttataaaaat
     9841 acagttcttt aatattaatc cattacatgt ttctttcacc ctccaattct caaacttcac
     9901 aaactccaac atgcaaagaa atatatactt taagaaattc agtccattaa tattactaca
     9961 aatacttttt agatatgttt aaaaagaaat ttcacctaaa atgtttataa aagatttaga
    10021 aataggtctt gtgtctattt taaaatatat aaaacagtgg taaagactta tttataagat
    10081 aaaaaatgaa tagtgtatac cctttttaaa aagttttttc ctttttatat ttgtgataca
    10141 atgttaaata taaaaaggaa aattttgtat taaagaaatc ttatttgttg attttgtaac
    10201 ttcaaactca gcatcaattt gattttaaga ttatatttta ttaagtatgc taaataacaa
    10261 atttttaaag attgtgaggt aaaaatatga ataagaaaaa tttagctata ttaatatcag
    10321 gtatgatgtt tttttctagt tcttcagcaa tttttgcaga caatactaca aagcaagaaa
    10381 gattaatagg taaaacaaga tatgaaacag cagtagaagt aagtaaattg ggatgggttc
    10441 aatcaaaaac agccattata gttaatggta attctattca aagtgcgctt tgtgcaaatc
    10501 catttgctaa gctaaagaat gcacctatac ttttagtaaa caacaacagt attgaaaatt
    10561 caactaagtc tgagcttaaa aggcttggtg tagataatgt gtacattgta gatagtggaa
    10621 atagcatttc ttctaaagta gaaaatgaaa taaaatcact taacataaaa ataaataaaa
    10681 tagtaggaaa caatatatat gaaatgtcta caaatgtact taaagaaata gataagataa
    10741 aaaagataga aaatgttgca gttgtaaatg gactaaaggg actatctgat gcagttagta
    10801 tagcttctcc agcagcaata cataatatgc ctataatatt agtatcagat gaaaacagca
    10861 atgatagtag tataaatttc ttaaacaata ggtctataaa aaaatcttac atagttggag
    10921 gaaataagat tttatcagac aatacagtaa agaaatatcc aaacagagaa agaatcagtg
    10981 gttcagatag aaacaatact aatgcaaaaa ttattgataa attttataaa gataaaaaaa
    11041 taaataatgt atttgtagct aaagatggta ttaaccaaga aaatgcatta gtagattcat
    11101 tatcaatagg agtactagct gctaaagaaa attcacctat aatgatatct aatggtagcc
    11161 ttgtaaatag tcaaaaagaa tttattaaaa ataaagatat agatgaaata acacaagtag
    11221 gaggaggata taatcaaaaa gcatttgaag atattttaaa actaaaaaat gtgaacataa
    11281 gaaataaaac taaaaaaata tctttaatac aggctaaaga aatattaaaa caagctcttc
    11341 caggaagcaa tattttagtt tgttattatg atgaagatga taatgaatat gactgcaaag
    11401 ttataaagga taatatccta tatgagatag aaattgatgc ctactctggt aaaataatag
    11461 aaattgaaaa agatgatgac ttaaatgata ataatttagg tacacaaaat atatcattgg
    11521 ataaagctaa gtctatagca ttagatgctg ttcctaatgg taatttaatc tattgtaaat
    11581 atgatgctga agataacgaa tatgaggcta aagtaataaa aaataagaaa acatatgaat
    11641 tagagataag tgcatttgat ggaagaattg tagatattga catggacaat gattctgatg
    11701 atgactttga tgataatgat aagaataact ctaatactaa tgacaacagt tctattatta
    11761 gtgctgaaaa agctaaatca ataatgctag ataaagtgcc tggtgggaaa atagtaaaat
    11821 ttaagtatga tgaagatgac aaagaatatg aaggtgaaat aataaaagga aaaactgaat
    11881 atgagataac aattaatgca attactggta aaatattaga atttgaaata gatgattaat
    11941 aaaaaatata tattctatta attattacaa gcaataaagc cataaatatt gaaattttca
    12001 atatttatgg ctttatttta ttttttttaa tttaaatcca cattatgcca ttatatatat
    12061 ctatgcactc ttttaaatta aaatcttgtc catcatccca aacctaataa agttagattt
    12121 taaacaatat attgtgatat aagtaatttg tgtagaatta ttgcgtgatt tactcatgaa
    12181 gaaacaataa ggttaatata taatttgact atattaagac gaaaggagac atatacaatg
    12241 aatacaatat taaaagtaaa taacataact aaaacttata ataaagggca acagccgagt
    12301 ttagataagg tatcctttaa tgtatcagaa ggtgaattta taggtataat gggagcttct
    12361 ggctctggga aaacaactct acttaatgta ttatctacaa ttgataaacc aacaagtgga
    12421 gatattttca taaataatat aaatttgaaa tcgcttaatg acacttgtgc tgctgatttt
    12481 cgtaaggata atttaggctt tatctttcaa gagtattttt tactagatag tcttacaatt
    12541 ttcgagaata tttcagtacc tcttacactt cagaaactca atccagataa aatagaaaac
    12601 atggtaaaga gtctagctga acgatttggc atagcttctc aattaaataa atacccaaat
    12661 gaattatctg gtgggcaaag acagagagct tcagcagtta gagcaatagt aaaacatcct
    12721 agtattctct ttgcagatga accaacagga gctttggatt ctaattcagc tactgaacta
    12781 ctccactcac tacaggaagc aaatgaggag ttgaaaacaa caattttaat ggtaacacat
    12841 gatgcctatg tagcaagttt tgcaaataga attttgattt ttaaagatgg tcatatcatt
    12901 aaagaattaa ataaacaaca gaataataga catacatttt atgagttgat tattcaagaa
    12961 attgcaaaat tggatactaa aagataggag gataatttaa tgaacatttt ttatacggca
    13021 ataaagaatc ttaaaaaaaa cttttcattc tattctctat atctactttc agtctctttt
    13081 gttattactg tatttttcgc atttacttca ttttctgtta ataagataat gctagaaaaa
    13141 atttcaactg atggtagagt tgaatctatg tgtaatgtta tttcgatatt tttgatggca
    13201 tttgtcatct tctatatggc atactcaaat cgattcttct tgagacgtcg tacaaaagaa
    13261 ttaggtatat atgctttact tggctatcgt aagtttacaa tattatcgct acttaccttt
    13321 gaaaatgtat taatttgtag tggagctttt attattggat tggtattagg agcattggca
    13381 cataagggta ttgtctttgg aattacagca ctacttaatt tatcaattga taattcacag
    13441 ataccatttt ttaatataaa tgcaataaat aaaacaatta tctttatagc attagttgtt
    13501 ttagtcttag ctgtatcaaa tggtaaattt ttttataaga ctccacttgt aaaattgata
    13561 agatatgaaa aaagtgcaga aaaaaaatta aagttcagaa aaattccagc ctttatgggg
    13621 tttattatga ttttaagcgg gtattgttta tcactagata ttcttcgtgg agctaaatca
    13681 gtatgggtaa gtattggatt ttatcaaatg ggcttgataa ctatgattat cattgtaatt
    13741 ggtacagttc tctttatcaa ttcctttctt ccatatatta tgcaaaatag taaaaaaaat
    13801 aaacgtactt tctacacaga tatgaagata atagctattc caaactttat ctatcgtata
    13861 cgttcaaact ctaaaactct aattatgctt acattactct ctgctactac tttgacagtt
    13921 tcaagtgtta tggcactgtc cttatactac cctattgcat ctgtttcgcg tattgcacct
    13981 tcagagatag aatttagagt agaaaaaaat aaacaaatag aagatgtaaa acaaattgtc
    14041 agtcaatata cgtctaatac agatgatatt atttttacac agacaaatat ttataaggta
    14101 aaatcttcat caaaagacct tccatttgaa tatgaaattg gtacatctaa gaataattta
    14161 tccaatagta gttcttctaa agaagttatt tcaagagaat caggatttga atgtatatct
    14221 ttttctgatt attatacttt attaaaggca caaaatcgaa caaagctact aaaacaactt
    14281 acccctttaa gggataatga atgtatttta atgaaatatc aaccaaataa tgatggatat
    14341 agtgaaaatg gaacgattta tccattactt gttaatggta aagaaacacc tgtagctgta
    14401 agggaaacat cattaaacaa tgctatttct tttgccaata gcgttggaac acttatcgtt
    14461 aatgatagta tatatgagca aatagcacaa aataaattac caatgacaag cattttaagt
    14521 attaatggta aatcaattaa aaataatgaa aatttatata gtaatatttc agattattta
    14581 gatggaagcc cttatttaca aggatattct cacaggataa atgatttgtt atcaattaat
    14641 agttctacat ttctgttaat cggatttctt gtggtattat tctttattgc tgttggaagt
    14701 attttatact ttaacaatat atcagcaatt tcagattcaa catctgatta taagatttta
    14761 cagaaaatgg gttatgataa tatacaaatt aaaaaaatta ttaaaaaaca agtattgcca
    14821 ttttttaata ttccgttttt acttggtata ttggactgta tatttgctac cttggtttat
    14881 aagactgctt tgatgcagaa tttacttgga aattcatttt cacaatacat acctgtgatt
    14941 attgcaattt tcttaacttc tgtagtgtat ttgatatatt atatacttac tattaaatcc
    15001 tgttgtaaaa ttgtattaaa aaattaatat ttaccaaatc gtcaagctta gaggtgtgac
    15061 gatttggtat acttcattta taagtatttg atataattta aaattataga ttcttaggtt
    15121 tgatttagtc ataaaattaa tataattaac tcataaggag gtgtataaga ttgacaaatt
    15181 ttaatttaaa aatactattt ttaggaaact acattctatc acttctcttc atgggaatat
    15241 ctacaagcac tgaaacttat atgaatatcc aaagcattat tcccttaata atttcaacat
    15301 tattactcat agacatcaca tatataacct cttgtaatgt ccgagaaaat aacattattt
    15361 cattgttttg tggattacta gcactagaca gttggtattt actcttatca tttgtaacca
    15421 caccaatagg cactattatt tttaaaatat taagcacagt tatagtgtat gtctctatta
    15481 aatttatatt tttatttttg tttcaaggta gtggatacaa tttccgtaag ataacagaca
    15541 ttttattgct tatcacctgc attggtgcaa ttgtaggagc atttatttct aacagaacgt
    15601 atgcttgtat gtacggaata cagtttgttg ttagtataat aagctttttc tatattattt
    15661 tttatcattt gaaaagggtt gtttttgtca taaaaagcga aaaaaaacct atatcgattt
    15721 cagtatttat aacaattata ggattcctta cttattattt ttttacacta agaatatcaa
    15781 atcatattgg taattttgga atctatctaa ctgtactggt attttttatg agtattcatg
    15841 gtattgcact taaagaaaat gatagttttc ctatttcaac agtttttaac ataaaacaaa
    15901 gggctatatt tctttttata agtattttag taattagctt ggtcgtacta tttgccaatg
    15961 ggaatttttc tataatatta atagctataa atgttttatt tatgctcatt ttcctctgta
    16021 acattatttt ggagtttaac cttagagatg aaaaaaataa agtagttaaa aatagcaagt
    16081 atacttttgc attagaaaaa ttacaacaag aagaacaact aaaaacagag ttttctaatt
    16141 ttttacatga tgacatatta cagaacctgc tttcaataaa aaacatgatg agtaagtcat
    16201 atcgcccaga tgttcaagat attatatatg aaacacttaa caatctaaat acatgtataa
    16261 ggaagcaaat gcaagactat catcctgtta ttttaaaaaa tttaacagta aaagaaaatt
    16321 atcagaattt aattgaaagt gtttcttcat cctttccaca aaaaaatatt tgtgtttcat
    16381 ttaagtgttc tgatacttta ttcttagttg aaccatatga tatattgata tatcgactga
    16441 ttaaagaatt attgacaaac gtatataaac attcaaatgg tagccatgca tggatagctt
    16501 tagcactaga aaaagatata ataaaattaa atattaatga taatggtgtg aaaaacacat
    16561 cttttaaaac acctttagat ataactaaaa ttacttttga tacaataaaa cacaaaggga
    16621 ttatatcaat taaagaacaa gtggaaaatc taggaggaac gataagtatt tctgataata
    16681 tacctcatgg agtttgtgta cagattacaa tccctatgaa aggagatggt tcttataaat
    16741 attttgttag ttgatgacca tatgcttttt gctaaaagct tgtcaattgc actagaagaa
    16801 tacaaagaaa tcgaacagtt ttattccaca caggacatcc ataatctttc agaaatgatt
    16861 caagataaaa aaatagacat tgtcttaatg gatataaacc ttagaggctt aagtgaatgt
    16921 gatggcttaa aagtagctta taatttatta gagactaatt caactattaa aataataatc
    16981 ttgactggat acgacctccc agtttataaa catgaggcta aaaaaatagg tatctatgga
    17041 ttcctcaata agaatattag ccctgatagt ttaattgatt cattattaaa agtctatcaa
    17101 ggggtaactt gttttccatc tgaaaatact gaagtaatta ttgaagattt aactactatg
    17161 gagaaaagaa ttttacagct tatctgtagt ggtaaaaaaa gaaaaataat tgctgatgaa
    17221 ttgtatataa gtgaaagaac attatcaaat catttacaac acatatatga caagctagat
    17281 gtttcatcat cagttgaggc tattacaaaa gctattaaaa tgggttatat ttctccagta
    17341 tattaaaaaa agtgagtgtc tcaaaataaa tcttctaact cataaagcac tctcctttgt
    17401 attaaatcaa atatattttc atcatttaaa caattaagaa gaggcttctc tctattttga
    17461 gacaactcca ttttaataca ctatttatat ttatatataa acatatttta aacaactaaa
    17521 aagcaactaa tatctcaata catatcttca taacacatat caccataatt acaagcagat
    17581 aaagaactct cctcatttaa atacgactca aattcatacc caaacagaat tgttggttct
    17641 atatatattt ccatatctgt atatctccat cttcttacct ttgcctctat aaccttgtaa
    17701 aaatcttcta aactataccc ttcttttaat cttttatcta taagtgcctt agttatactc
    17761 aaatcagctt taaaattttt tcctgattca ctatttaatt ttcttataat aatttcatgt
    17821 atgctttctg gatttttgta agtagaatct tcagtcaaat cttcatccga attttcaaag
    17881 gtatttttat ttaaattctt tttatttaaa ttatttttta ataattcttt tttctttgtc
    17941 tcattttctg tttcataccc agtatgaatt atcaaatcat aatctttgtc tatactctct
    18001 tgatttatac ggctgtatat gttggtatta ctgttcttaa acaactcatc tctatgttta
    18061 tatttcattg ttttttggtt attgttaata gttgcatcaa tgtatttctc tatatttttg
    18121 aaaatatcac cattctttgt tacaatttct gcattactac tttcagaaca aacataacta
    18181 tatacagaac aacaatttcc ctttactccc ctacttatta atctcaatat acctaaatct
    18241 ataaattcat ttacaagtct acttattgta gatatagata catcaaggtc agatgcagct
    18301 ttccttactg tcatcataaa ttgtcctctt ggcagagttc cttttaatct ttgtatattt
    18361 tcttttctca ttacatagtg attaaacctt attctattta catctctttt ttcattaaaa
    18421 tataatactg ctgaatttaa ttgataaaaa ctattactca tattcccact cctccaattt
    18481 aaatagataa tttgttgtaa agtttttaga tttttaaata acttattaaa tgattatttt
    18541 tattaaaaag tattctttga tattttcatt ttaaagtttt aagtataata agttaatagt
    18601 gtagatttaa cttttttatt taaacaataa acttaatatt atttttaata taaaaatttt
    18661 attaaattt
//
LOCUS       DAFKMX010000072        18660 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFKMX010000072 DAFKMX010000000
VERSION     DAFKMX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.072"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(52..1356)
                     /locus_tag="LZB08_001931"
     CDS             complement(52..1356)
                     /locus_tag="LZB08_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBM7315435.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(1713..2588)
                     /locus_tag="LZB08_001932"
     CDS             complement(1713..2588)
                     /locus_tag="LZB08_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBM7315436.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(2781..3467)
                     /locus_tag="LZB08_001933"
     CDS             complement(2781..3467)
                     /locus_tag="LZB08_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBM7315437.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(3516..4049)
                     /locus_tag="LZB08_001934"
     CDS             complement(3516..4049)
                     /locus_tag="LZB08_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBM7315438.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(4093..4725)
                     /locus_tag="LZB08_001935"
     CDS             complement(4093..4725)
                     /locus_tag="LZB08_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBM7315439.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(4815..5894)
                     /locus_tag="LZB08_001936"
     CDS             complement(4815..5894)
                     /locus_tag="LZB08_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBM7315440.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(6027..7373)
                     /gene="pepV"
                     /locus_tag="LZB08_001937"
     CDS             complement(6027..7373)
                     /gene="pepV"
                     /locus_tag="LZB08_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBM7315441.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(7442..8722)
                     /locus_tag="LZB08_001938"
     CDS             complement(7442..8722)
                     /locus_tag="LZB08_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBM7315442.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(8849..9109)
                     /gene="hfq"
                     /locus_tag="LZB08_001939"
     CDS             complement(8849..9109)
                     /gene="hfq"
                     /locus_tag="LZB08_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBM7315443.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(9223..10158)
                     /gene="miaA"
                     /locus_tag="LZB08_001940"
     CDS             complement(9223..10158)
                     /gene="miaA"
                     /locus_tag="LZB08_001940"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBM7315444.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEKEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(10196..12163)
                     /gene="mutL"
                     /locus_tag="LZB08_001941"
     CDS             complement(10196..12163)
                     /gene="mutL"
                     /locus_tag="LZB08_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBM7315445.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(12174..15017)
                     /gene="mutS"
                     /locus_tag="LZB08_001942"
     CDS             complement(12174..15017)
                     /gene="mutS"
                     /locus_tag="LZB08_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBM7315446.1"
                     /translation="MQIDINKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHDLIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(15178..15912)
                     /locus_tag="LZB08_001943"
     CDS             complement(15178..15912)
                     /locus_tag="LZB08_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315447.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKSYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(15881..16894)
                     /locus_tag="LZB08_001944"
     CDS             complement(15881..16894)
                     /locus_tag="LZB08_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315448.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(16916..17686)
                     /locus_tag="LZB08_001945"
     CDS             complement(16916..17686)
                     /locus_tag="LZB08_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315449.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(17661..17951)
                     /locus_tag="LZB08_001946"
     CDS             complement(17661..17951)
                     /locus_tag="LZB08_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBM7315450.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(18082..18181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5895 a         3218 c         1897 g         7650 t
ORIGIN      
        1 gattttgtgc atatatagaa agaccgcttc atttttagtt acttaaatca cttaatagta
       61 gttatttaaa tcacttgata aaggtttttt ataaaattca atagttctat tccatgattc
      121 aaactgtcct tttgaatttc cttctacagt tccacctgaa gaaaaaatcc ctccactcat
      181 actcatacat aaagtctcct ttagaggcat aatatatgga attggtaacg gatgacccat
      241 attattgtat gttaataatt ctacgttata tggataatta ttttcttcaa agatttctaa
      301 tgcctttaaa caagcttcat aactattcca tatgttatct tcttttccag caatcataag
      361 tatatctgct ttggaatttt ctatttttat tctagcctct tttctattct ctgctctttt
      421 aagacttttc ttataagtac tattaaatcc aaatggaata ccttttgata tatttttttt
      481 ctgttcttca aagaatatat cattgtctac tttgatatat ggaaatggct ctcctttata
      541 tgaccatgat gaagtatcat ttccactcat caatccatta agagcttgga aacaataaat
      601 gtgaggttgt gatactacaa cctttttaat ttgttcatat cttgaagcaa gtaaaaaggc
      661 caattctcca ccctttgatg tcccatgtac aaatatttct ttagtactgg taatttcatt
      721 ttcactaatc cactcaaata ctttttcaaa atattctaat ggaacctctt ctaatttatc
      781 tggtaaccct tctaaagcaa agtatgcaac tgttagtaca ttgaatcctc tagatgcaag
      841 tggagctgct aaaaggtcta gagcgtctaa atttccatca gaaccaccta gcattaaaat
      901 tgttttatta ttagaatttt gtttataaaa taattttcct ataaactcat cagatatgta
      961 ttttgtggta atatcttctg atttaaatat tctctttaat tcaacttctt ttctttcaat
     1021 agaagtttca aatatcatat tcataattat tggtttatca atagatatat tttctgcaaa
     1081 atttttacct tcagttttag atttttccag cgaataaatt aaccccatac aattatcagc
     1141 cttataagta ccttctatag gttcaactaa atctaaatct acttctcctt tttcatttga
     1201 acaaaacact ccataagatg aaaattcttc tcccttacac catggaaatt ccattttcac
     1261 acttatttta agtttagtat ttggaattaa gtctgaaacc ataatcttaa gtctttcatc
     1321 cacaaatgat tctacattgt atacatttac tttcataatc tatttctcct tataataatt
     1381 atttatgttt tatataaaac agctttatct aatacaagat aaataaattt ttaaattaag
     1441 acaccatatt gactatttca ttttatcaac atatttcatc tttgtcaaca tattgtcttt
     1501 tttagtaatt taaaattatg gtctatatga aatccagttg atttttggct aattaaagat
     1561 aggaatgcta ttaggtttag aatatatata ttcaaaataa aaaaccactc atttaaagaa
     1621 tggtttttaa tataaactat ttgttattaa taaggatatt tttttattta aatttatata
     1681 atcgaacatc taatatttaa tgtttaataa aattatgcat ttgcaaaagt atatatggca
     1741 ttaactcttg ctacatataa attctcatca ctacatccaa tatatctagc atatttttca
     1801 atcttttctt ttgcattttc aacatcattt tcatcgctct ccattaactc atttatataa
     1861 atatccatta atgacgcttt atattctctt atatcatagc ctaaaaattc agtcaatctc
     1921 tcaatttcct tactaaaatc tccatcttct tttaccaaaa attcgtatac atcttcccta
     1981 tatggattta aagttattat ttgttttact actttgcatt cgtcagcctt cccaataaaa
     2041 ccatctttat aattattaaa taaggcatta gattttttta tagattcttc atcaatataa
     2101 gaatgtaccg cagttatacg attttctata agggcatcta caacagcata atgaatttta
     2161 aaacaatcat tgtataaata attgctcatt ttctttattg tcgattctat ttctaagaag
     2221 ctagcgtgac catttttttt atttttattt ggtataataa aagttttatt caaattatta
     2281 aaaagtctct taaaatctaa atatttttca caataaacac ttttaaaggt atcgatatct
     2341 atagttataa ttccaaaact tactatagtc tcaattcctt tcttaaaaga ctcttctata
     2401 tatttttctc ctaaacttaa acctctatca gatatttgct ttagattttg aaaactattt
     2461 aaacaatctc tttcaaagtt tttagatatt tctaaagcat ataatttaaa gattttcctt
     2521 atcttattat aattattttt tgtatcatca aatctgataa tagctccatt tacatcaaaa
     2581 gtaatcattg agttttaacc tccccattag tttcatcaat aaaataaatt taatataaac
     2641 tttataatca taatattaaa cttatttata ctataattat taatttgatg ataaaagcta
     2701 ttttccttct aaataaaaaa tttgtctaaa gtttctaaaa aatttagaca aatcttatgt
     2761 tattactctt taaattattt ttaaattatt ccaaatacgc caaataatat tggtataaat
     2821 atagctggta acaaattggc tacttttata ttcgttaatt taagtacatt tagaccaagt
     2881 cctataatta ataaacttcc agctgctgtc atttctgtga ctacaggatc acttaaaaat
     2941 ccatttaaaa atgatgctcc cacacatata ataccttcat acaaaaatac agatattgca
     3001 gaaaacatta cacctatacc taaagaagca gtaaatataa ttgaagctat tccatctata
     3061 acagatttta caaataaagt atcatgactt cctgtaagac cactttctaa tgaaccaact
     3121 actgccatgg ctcctataca gaataataaa ctagatgata taaaaccctc tgctatagaa
     3181 tcttttttat ttcctttaga aaatttactt tgtaaaaaag cacccaactt atttacccat
     3241 ttgtcaatat ctattaattc tcctatcaga gcacctacag ctacggatat tattgtcact
     3301 aatgtgtttt ttccttctag tgctcctgat ataccaatgt acaatacaca taaagcaatt
     3361 ccattcatta tagtatcgct tactctttga ggtaatcctc cttttattat aagacctact
     3421 atacatccac ctgcaattgc tagactattt gctattactc caaacatttt ttctcccctc
     3481 gattagctaa ttgaaattta tcctattatt aaaacctaaa catctatact ttgtattgga
     3541 tattttaaat attccaaaaa ttcttcaaaa ccttttctag atatatttac agttttagta
     3601 tttatgttag gatgaaaatt taagtaatct tctttaatta catctttatc tatgtaaagc
     3661 tcaacttttc tttctgtatc atttattaat gaaaaaggat ttacacttcc aggaagaagt
     3721 ttaagtacat tgtatagtct ttcaggtgaa gcaaacgata atctagaact tcctatctta
     3781 tctttaagtg agtttaaatc aacttgtttt tctcctttaa ctacaataag ataatactta
     3841 tttcctttgg catttcttaa aaataaattt ttacattgac acccctttgt tattccatct
     3901 aaaatgacta attgctctgc tgtatataca gcttcatgct ctactacatc atattcaaca
     3961 tttaatttat ccaaaatatc atatatttta gtttcattca tgctagattc attttcatct
     4021 tgttcttttt tattatttaa atcactcata cttttctcct taaacacttc atcataatat
     4081 cattgtgagg tattattcta ctatagctat tgctcttact ggagctccat cagcatcatt
     4141 atacttaaat ggtgctgcta taaatagaaa tttttctgga acattttcta aatttgtaag
     4201 attttctatg attatttttc ctttttcaaa taaaatatga tgcacttcaa atactgatgt
     4261 gtcatatcta tccactgata gcatatctat tcctatgcct tttatattag tattagctat
     4321 atagtttgca gcttcttttg ttaatgtagg ataacctaca tagtactgct ttttatccca
     4381 aaattttgac caacctgatt taaatattat aaaatcacta ttttttattt catcttcata
     4441 tttttttaga tattctaatt ctatattctc atttcccttt tcaagttcca atacataagc
     4501 tttcccaaca aaattttcta tatccaactt atctagggtc tcacctttag tatacatatg
     4561 tcttggtgaa tccatatgag ttccattgtg agaaaataca ctaattaatg tttcttgata
     4621 accattttct tcaataatag ctactttctt gatatctggt ctattaggct ctgcatatac
     4681 aggcatgtca ttatgtgtca catgagttaa atcaaaaacc ttcatcttta tataccccct
     4741 atgtttttat tttctattat atacctaaat tcattttatt tctatcagat tttattccat
     4801 tatataccat actactatat attttttata aacctcaagg gatttaccga aaaatcttgg
     4861 aaagaattat cagtaaatat aacatcttca cccaaaaaac gtttatacct tccttcttct
     4921 ttctttaaag taattctctg tttttgtgaa tgattattaa aaaccattat cttaagagga
     4981 ggtttttttc tattagtctg cactcttttt aacatgtatt ttatgtgcat atcagcttca
     5041 ggaaatgaat atccacaaaa tattaaaagg tcagtatctc ttaaagtttt ttctgtttca
     5101 ttccaaacat tacttaaaaa tatgtttgac atattcttaa aatatgttgg tggtactata
     5161 ataggttctg ttaactcacc acactctaaa cattttgtct ctgaactatt ttcaataagc
     5221 ttcatgacac caccttcatg tggtgtaaga gttacactat tacaaatggg gcaataaagc
     5281 caattcaatg acccatgtat cttatataat ttgattaatg gttcttttgg ttttctccaa
     5341 tttaactcct ctttaaaatt tgtaaactcc acaccataat caagcataat tggcaaatca
     5401 ttcttatctc ttaatccagt aactgtattg tctatatgta tatcataatt tgcactaata
     5461 aaagtagtat caagaagcaa atcattttcc aaaagatttt caactaaaag tttatgatat
     5521 ttattatctg ttctaggtgt attatgaatt gcttcagcca ttagcagtat taaatattgt
     5581 cttaaaaaac gtatactatc acttctgtta tttagatttt ctagtccaaa atttctaaat
     5641 gcttcttttc tttgttctgc taagtctaaa agacctaaaa cttcttcaaa ggtaggaaaa
     5701 tttgcattat ctatattatc ttttactata tcaatattga acatctgttt aaagaaaata
     5761 tacaattctc tattcatttt ggtattataa tttgtgggat ttagtttttt aaaatattcg
     5821 ctaaaaagtt cattttgaat aggtagattc tctgctgctg aagctcctgc tcctaaaaat
     5881 attgctattt tcatatagtt cctcctaata taaatattac tcctattttt atatccatta
     5941 aatatccata tcatttattt tatgatacaa caataaagct gtcttttact agaaaaaatc
     6001 ctattacaag acagccttat attatattaa atctttaata attcgtaaat agaaagagca
     6061 tatatttgag tagcttttat aatatcctct actattaagt attcattagc ttggtgttct
     6121 gtttctggtt ttcctggaaa aatagctcca aatgcaacac aattatctaa agctcttgcg
     6181 tatgttgctc ctccagatga aagtggtgtt ccatcaagac cagtttcttc ttcatatact
     6241 tttctaagtg tcttaacaag aagagtatct tctggaacat agattgaatc caagaaatca
     6301 tattcttcat aatttaaatt gtatttatct gtcattttct ttaactcttt tacaaaatca
     6361 tcctttttat atgttactgg aatccttaca tctattcctg caaattcttt ttcgttatca
     6421 atagttactc tacctatatt tactgttagt ttacctgata catcatcctt gcaattagga
     6481 attatattgt ttccattagc atcttcacca ataacttctg ctaaaaactt aattatatta
     6541 gaatctatac ctatattatt aagagctata caaagtctag ccactgcatt tataccagta
     6601 tcacttgctg ctgaatggac actttttcca attatacaga ttttattacc ttcaacagta
     6661 tactcaaaat ttaatttatc aagttctttt tttaatttat cagagtattt tccaatatag
     6721 attgctttac ctggaactgc atttaaagcc tttccaactg acaactctat atcacttttg
     6781 ctatcacaag tcaaatgaac ttgtaaaaga cctttctctg catttgttat tgggaatcta
     6841 gaatctggag taaatccata gtttggtatt tcttcattat tttctttata tttatttata
     6901 catctccaaa gattttcttc atcagcccca aaaatgaatc ttatcctctt attaaagtct
     6961 acatttaaat ccataagagc tttcactgca taaattgctg atattgtagg tcctttatca
     7021 tcttgtgttc ctcttccata caatttacca tcttctaaaa cagcttcaaa tggagggtaa
     7081 ttccagcttt ctaaatcacc ttctggaacc acatcaacgt gaccaagtat acctatcatt
     7141 ttttcacctt gtcctatttc tgcatatcca taatatccat ctttgtatac tgtcttaaat
     7201 cctaaagatt tacataagtc taagatacct cttaaagctt tatctacatt ttcaccaaat
     7261 ggacaatttt cagttgcttc agatataaca ctaggtattt ttacactttc ttgtattgaa
     7321 gaaatcattt catcttgtag agaatttact ttttctttaa tttgctcttt catattatca
     7381 cccttcaatt ttcatcataa tatactaata aatattttga cctttaaaaa aaaaaatatg
     7441 cttatttttt cagttttttt ataaattcaa tcgcttttaa agtccccatt tttgaatggt
     7501 caaatgtaag tccaccttga aaataaacat tataaggagg tcttattgga gcatctgcac
     7561 ttaattctat agaagaacct tgtataaatg ctcctgctgc cattatgact tcactttcgt
     7621 atccaggcat gtcccaagga acaggtttta cataagaatc gactggtgca gcttcttgta
     7681 taccttcaca aaaacttatt acttcatcag cattattaag tctaatacat tgaataatat
     7741 cacttcttaa gtcatcatat tttggtaata catcatatcc taatttttca aaagctcttg
     7801 agcaaaagat agctcccatt acagcctgag atactatata aggcgccaag aaaaaaccct
     7861 gaagtacatt tctagttgtt ccaaaagtaa gaccacattc ttttcctatt ccaggagaag
     7921 tcattctata agatataagc tcaatcaaat cttttctacc agctatatat ccacctgtca
     7981 atgcaagccc accaccagga ttttttatta aagagccagc cataacatct gctccaacat
     8041 cagtaggctc tttggtatct aaaaactcac cataacaatt atcaaccatt actatagctt
     8101 ctggtttaac agactttatt acttctatag cttcttttat atcactaatt gaaagtgatt
     8161 ttctccaaga atatcctttt gacctttgta tcataacgag tttagttcta tcatttattt
     8221 tgtttttaat tccttctaaa tctaagtttc catcctctaa aaaatctaca tcatcatatg
     8281 taacaccata ctctttcaat gaaccttttt cttctcttat gcctataact ccctctaaag
     8341 tatcatatgg tctaccagta acagataaaa tttcatctcc tggtctaact ataccttgta
     8401 tacataaagt aagtgcatgt gtaccattta caattattgg tcttacaaga gcatcttctg
     8461 tattaaatac tttagaataa atttcctcta ttttttcacg accaatatca ttatatccat
     8521 atcctgtagt ccagttaaag tgcatatcac ttagtttaga ttcttgcata gcttttaaaa
     8581 ctttatattg attatattct cttatttctt ttatttcttc aaacttatct tttatttctt
     8641 ccattatttc ttgcgatagt ttaaaagtat catcatctat tccatagtag tcttttaata
     8701 attcttttgt ctcattaagc attttatcac ctgtctcttt attattttat ttaaacatgt
     8761 aaaggagact attttactaa aacaaataaa ttatagttta taagttagta taagtctcct
     8821 ctcaatcatc ttaaattaaa ttaattatct atctgttgtt attattattg ttgttttggt
     8881 tttgatttgt tagattaata tactttccag gttgtatagt tgatacagca tgtttataaa
     8941 tcatgttttg ttgtctatta tccccttcta ttaatattat ataactatca aaccccttta
     9001 caagcccttt aacttgcact ccattaacta aatatatagt aacaggtatc ctttctttcc
     9061 ttgcattatt taaaaacaaa tcttgtaaat ttaaaactgt atttttcatt agatttgtac
     9121 cctcccaaga ttaaattgaa cttctaaaat attcctacct tatattctat ataagttatg
     9181 acaaattttc aatattaagt tgtatactta aaaatttata tattatttta tagaatcttt
     9241 aatatataag attatttcat tttttaattc atctatattt tcatattggt ctaaatcaaa
     9301 ccattttgct gttttatatc ttttaaacca tgttatctga cgttttgcat accttctaga
     9361 atctctttta attatttcaa tagcttcttc gtatgtatat tctccatcca gatactttaa
     9421 tatctcttta taacctatac cttgcattga tatcatatct tttttaaatc ccatacttaa
     9481 aagttttttt acctcttcaa ccaatccatt tttaatcata atatcaactc tcatatttat
     9541 tctttgatac aaatgttctc tatctctatt tagaactatt attattggtt gatattcttc
     9601 attaaatttt aaatcacttg aaaaatcatt cattttctta ccacttagac aaacttcaag
     9661 agctcttatg acccttttag tattgttttt atggattcta ttagcagatt cttcatcaag
     9721 ttcttttaac ttattatgca tgtaatctat tcccttttca gcaagttgtt tttgaagttc
     9781 ttctcttaat tcattattgg catcagattg tgcaaaatcc atattatata ttaaagagtt
     9841 taaataaagt cctgtgcctc cagttactaa aacacatttt tcctttttat ttatttcatg
     9901 tatatatttt tttgctcttt gttgaaactc tgaaactgaa aatggatagt caggcttaac
     9961 ttcatctata aggtaatgct tcactccttg catctctttc tctgtaactt ttgcacttcc
    10021 aatatccata tattcatata tttgcataga atcagctgaa attatctctg cattcatatc
    10081 ttttgctaat tttattgaca aatctgtttt tccaacagca gttggacctg taagtattat
    10141 aagtggtatt tttttcataa cattcctttc catatatttt aaatatccaa atattttaca
    10201 taattctttt aaacattttt tctatttctg tcttagatat ttcgaccatt ataggtcttc
    10261 catgaggaca tgtaaatgga ttctcacact tttctagctg ttccaataaa ctttttattt
    10321 caatatcgta tattttatca tttgctttta tagcagacct acatgccata gatgcaaatc
    10381 tctctccttt taaatcataa ttacttgtaa tttcttctat attatcaatt atttgaagta
    10441 taaatttctc agtttcaggt actccaaata tagtaggaac acatcttacc attatatgat
    10501 tattgccaaa aatttctagc tcaaaaccaa atttcataaa taactccaag ttattttcaa
    10561 tttgaagcat atctacattt gatacttcaa taaccactgg gtctaaaagt atttgcatat
    10621 ttatatcttg tctatagaac ttctccatat atctttcata tagtattctt tcatgtgcag
    10681 catgttggtc caacaaatac atagaatcat ctttgctcaa aattatataa gtattaaata
    10741 caactccaat tacagaatat ccgtataaag aaaacttgcg ttttgaacta cacaagtatt
    10801 cttcttcact gtccttaata ctatctccta agtaataatt tccttcattt ttaattccat
    10861 caacttgaaa ctcttcttgt atattatcat ttgcactatc ttcacttaga taattaatgt
    10921 tttttacatc atcttctaca cttgcattta ggacttcact tactgattga aattcattta
    10981 ttggtttttc acttgataaa gtaacaactt caatgacttc atccttacct ttagtaatat
    11041 tactattttt aggagtagat tctaataaat cattatttct taatttataa tctgtacttt
    11101 tttctctact gttaatagcg attctaggtt gtgttttttt atcactataa gtagcatatt
    11161 ttccaataag atttgagtgt attaatttaa cctttaaaaa atctctaagt tctatataca
    11221 cttcttgttc tttttcaaat ttaatttcaa gtttattggg gtgtatattt acatctatac
    11281 atgatgggtc tacttctata tttaagaagc acactgcatg tttacctatt ggaattatag
    11341 acttataact ttctgttatt gcatctatga ttattttact ttttacaaat cttttattta
    11401 tatagatatg ttgtaagttt ttatttgaac ggtatatatt gttattacct atatatccat
    11461 tcattttaaa atgattgcac ttaaactcta catctattat attttccgtt atttcttttc
    11521 catatatact tctaatagtg tttacaagtt ttccatcccc tggagtattt agcatctgtt
    11581 tattgttatt tgtatattta aattgaatgt ttggattacc tattgcaagt ttatttatca
    11641 aatcacttat atttatagtt tctgcatgag tagattttaa aaatttctgt cttgctggtg
    11701 tattaaagaa aatatctttt ataatgatgg tagttccatt agtagaacct attggttctt
    11761 tcgatattat ttttccacct tcaacatata ttttagttcc tattatttca tctttagttt
    11821 tagtcgtcat ttctaatttt gatacagctg aaatagatgc caatgcttct cctctaaaac
    11881 caagagagta taaatcatac aaatcatcaa tttttttaat tttactagta gcatgtctca
    11941 aaaatgattt ttcaacttca cttgaaggta ttcctattcc attatctgta atcttaatta
    12001 gactttttcc tccatcaatt atatctatgg aaattttatt tgcacctgca tcaatggagt
    12061 tttcaataag ttcttttact acagaagaag gtctttctac aacttcacct gctgctatct
    12121 tatttatagt caaatcatct aggatgttaa ttatattttt catttttaca cctctaagat
    12181 tttatttctt ttgcttttct ttgaagatta taaagagagt ttatagcatc taatggagtc
    12241 atatttaaaa tatccagatt taaaatttct tctgatagta tatctctatt tacactatca
    12301 aaagagattt gagttaaggc tacctctttt acagaatctt cttgattaat tttctcttta
    12361 tcatcatttt gcttcattaa agcaacttca tcatttaatg ctttaaattt agaatttaat
    12421 ttatcataat ccttttttat atgtttgtag tcttttacta taaggtcatg ttctattttt
    12481 agagattcat atttattctt aaaattactt tgatttacct taactaattc ttcgtccaaa
    12541 ttactattaa ttatattatt gttttcttta tctccattta ttgcaacact gttgtaaaca
    12601 tgattttttt ccaagtcttt aagtatatat tttgctcttt ctatgacttc gtctggtaat
    12661 ttagctaatt tagcaacata tattccataa ctcttgtctg ctccctgagg aattattttt
    12721 cttaaaaata ttattccttc cccatcttct ttgactgcta ttgaataatt tttaacctct
    12781 ttaaactctt cttctaaatc agtaagttca tgataatgtg ttgcaaataa tgtcttacat
    12841 ctaatatttt tttgaatata ttccactatt gaccatgcta aacttatccc atcataggta
    12901 ctagttccac gacctatttc atctaatata actaaacttc tctctgttgc attttttaat
    12961 ataagggata cttcgttcat ctctaccata aaagtagatt gaccttgaga taaatcgtca
    13021 ctagctccaa ctcttgtaaa tattctatct aatattggaa tatcagcaga ctctgctggt
    13081 acaaatgagc ctatatgtgc cataagagct attatagcag tttgtctcat atatgttgat
    13141 ttacctgaca tattaggacc agttattatg ttaataatgt tttctccacg atttaaataa
    13201 gtatcatttg gaacgaaatt ttcctctcct actatatttt cgacaacagg atgtctacca
    13261 tttcttatat caagtttatt attttcattt attgctggtt ttacgtagtt atttatatgt
    13321 gcaactgttg caagggacac aaaaacatct atattagcta tagtcttagc cactttttgt
    13381 attctatcta tgtttttata tattgtatct cttatctcta caaatatctc gtattctaaa
    13441 gatttaatct tttcctctgc atgtagtatc ttttcttcta tttcctttaa ttctggagtt
    13501 atatatcttt ctgcattact aagtgtttgc tttcttatat atgtctcatc tagctttgcc
    13561 tgtttaaagt tagcttttgt tatttctata taatacccaa aaactttatt aaatcctatt
    13621 tttaatgatt taactcctgt tttttctctt tccctgtttt ctatttcctt tacaagaaag
    13681 gctccattct tagaaatctc tcttagttcc ttaagttcat cactaaaatc tgatttaatt
    13741 atgtttccat cttttatggt tatcgtaggt tcttctagaa tagcttcatc tattaaattg
    13801 tatatatcat ctagtttatc catttctgaa acatactctt ttaggatttt tgcatttgat
    13861 aaatttatag tatcttttaa attaggtaac ttttctatcg aattttttaa gtgaattaac
    13921 tcttttggtg ttactctctc aaatgctatc tttccacata ttctttctat atcgtatata
    13981 tttttaagta tatcatttag gtcttctctt aatataaagt catcttttat ttcttctatt
    14041 acatctaatc tattttcaat tttagattta tttatcaaag gttcttctac atattttctt
    14101 aaaagtctac ctcccattgc tgtagaagtt ttgtcaagta catgtaataa tgaacctttt
    14161 ttcttattcc ctcttatagt ttgagtaagt tctaaattag ttctagtaaa catatctaaa
    14221 accatatatt ctgatgaatt gtatatattt atattattaa tgttagatgt tatttgtttt
    14281 tgagtattgt atatataatt cagaagaatt gataagcttg actttattag acctttatca
    14341 tcaaacttta gtttttgtag ataaacatct ggaaaatatt cttttaatat atttatatcc
    14401 aaatagttat cactaaaact ttcatttatg tatatattac ttacagtagc tatatctcta
    14461 agcttttcta taaagtctaa atcatttata attatttcag ttggatggat ttttgcaatt
    14521 tcttcaatta ccttatcttc atttaaacat gttgcattag tttctcctgt acttatatca
    14581 acataagtta gacctatatt agtaccatcc ttatacaaag ataataaata attattcttt
    14641 ttattttcaa gtaaatttcc atctaaaact gtaccaggag tgatgactct tataacctct
    14701 cttctaacaa ttccttttgc tgtagatggg tcttccattt gttcacctat tgctacttta
    14761 tatccatttt caactaattt tgatatatat gaattggctg agtggaaagg aacaccacac
    14821 ataggagcac gttcttctaa accacaagct tttcctgtca atgctatttc aagagctttt
    14881 gaagctacta atgcatcttc aaaaaacatt tcataaaaat ctccaagtct aaaaaacagt
    14941 atacagtctt tatatctatt ttttacttca agatactgtt tcatcatagg agttaattta
    15001 tttatatcta tttgcattat caaacctcct tcttttctaa ttaaatatta tatcatagta
    15061 gatgattatt attaacacaa tatataaaat tttgacatat aactaaaaac aatccaaaat
    15121 gtatactcac attttggatt gctaagttta atgcttatta aataaaaata ttttttttta
    15181 caataaagat attatttctt ttttaagttt aatgatattt tcaaggttat cttcatttaa
    15241 ctttctacta tctataacaa attctttctt tactattgat ggtttttttg aaataacata
    15301 aattctgttt gatagcatta tagcttcgtc aatatcatga gttataagaa gtattgttga
    15361 atttactttt ttcttaacat ctaaaagcca tttttgcatg gatgttttag ttaatgaatc
    15421 aagtgcccca aaaggctcat ctaaaagcat tatgtcatta gaatttataa aagttctcaa
    15481 gaagttagct ctctgtctca ttcctccaga aagttctgaa ggatatttat cttcatatcc
    15541 gcttaatcca aatacctcaa aataagattt taccattgac ctagctttac tctttttttc
    15601 tttttttaaa attagtggta gtgacacatt atctattata gttttatatg gcaagagtaa
    15661 atctttttga tacatataac tatacttacc atttatattc actttgccac tatcataatc
    15721 tgtaagttta gttataatat taaaaatagt actttttcca catccacttg gccctaatat
    15781 agaaacaaac tcaccttcat aaacatcaat acttatatct tttaaaactt caatatcttt
    15841 ataagttttt gatacatttt ctatagatac ttttactttt ttattgtggt aaaaattcat
    15901 ttgtgtaagc atcacttgct ttcatatcct tatctattaa tttatattct tttaagaatg
    15961 atgtatagtt gttccataca ctatctttca tttctcccca tcgtggtgca tcatctttgt
    16021 acttgcttgc taaatattct tgactcttta aagctagttt ttcatctact tcaggtgcat
    16081 gttttactaa aatttttgct gattcttctg gattttttat tgcatattca taaccttttg
    16141 ttgtagcttt taagaatttt ttagcaagct ctggattatc tttaagcaca gtttcattag
    16201 aaattataag tggtgtataa taatccaatc tctcatctaa atcttttact ggtataaagt
    16261 ttaagtcata tccaattaaa tcagctttaa ctgcatccca accttcaaat atccatgcaa
    16321 aatctacagt tttcatagct gcaaagaaat catcttgacc agtattaact atctttaact
    16381 tattaaaatc agctccattt ttcttcataa ctgctttaaa aacagcttct tctgatggag
    16441 agccccaacc accataagtt tttccttcaa aatctttagc tgttgttata ttcttttctt
    16501 ttggtgatgc aaacccagaa gtattatgtt gtataacagt tgctacagct tttattggaa
    16561 gtggatcttc actagtcttt gcataagtaa cttgttcttg atagcttata ccaaagtcag
    16621 cttttcctgt tgctactaaa gtttctgctc cactttcagg tggttgtact atttcaacat
    16681 ccaaaccttc ttctttatag taacccttat caagtgctac aaataatcca gtatgatttg
    16741 tatttggtgt ccaatcaagc accatagtta cttttttaga agaagtatct ttatcattgt
    16801 ttttgtcttt attgttattt gaagaacatc cagttattgt tgccaatgta aaaacactta
    16861 ctaaacctaa tattcctaac tttttaaaat tcataattgc ctccctcaaa tacctttaat
    16921 ctattaatct atgtcttata acaacttttt ttacagcctg tacaattcct acaaacatta
    16981 aactaaatat aactactaat atagtagatg caaatacttt atcaagtgca tatgcacttt
    17041 ttgacctaag catataaaca ccaagtcctt tcgttccacc tagccattct gcaacagttg
    17101 ctgccattac agcatatgtt gcagatattt taagtccaga aaaaaattta tccattgcca
    17161 taggaaattt aaggtgaatg aatgtattaa ctttatttga gtccatagtc ttaaataaat
    17221 ttaaataatc tttatcaata ttctcaatgc catctacaaa acttattaat ataggaaaaa
    17281 aacaagtaag cattaccatc aatacttttg gcattgttcc aaatccaaac catatcataa
    17341 agagtggtgc tataattatt gtaggtatca tctgtgtaac caacataata ggataaagac
    17401 atttttttat tattgataca aaatccatca atattccaat taccaatgat attataattg
    17461 cgactataaa accaagtatt gcttctccta tagtaaccac agcatgcatg gataaatttt
    17521 gataatcttt aaaaaacaca ttaattatat ctactggtgt gggtagtata tactgaggaa
    17581 cttctaaaac tcctaccata gattgccaaa gaataagtat aaacaaaaat gttgctattg
    17641 gatatatact atctttgact ttatctatga tttctaagtt tttcatctat agtcagacct
    17701 ccagcttttt catctactct agctactgtt atgactcttt cacatccaag attaaaaggg
    17761 attttatgta ccttttttaa taattcaaaa acttcatcaa aatcaccttc aacagtagtg
    17821 ctcattgctc ctatttcaca tttaagacca cttttttcaa tttcagcaat acaagcatct
    17881 actactttat acatctcttc ttcacctttg taaggtctta gtggcattac tgctatgtct
    17941 gcgattacca tttaaatcac tcctttaaaa ataattaata cctactaggt atattttgtt
    18001 ttttattaaa aaattttatt actaaaaaga tattatattt aaataaaaga aaagtcttat
    18061 ccaaaatagg ataagactta tttttatctt aactggtctc cctacactgg cattatccaa
    18121 acaggttcaa agggtcgaga taaaatctcc tctcagcaca ttagctcccc gaaatatatt
    18181 atatcttttc tacttgatta tactacactt atcatatttt aacaaatttt ttatttatat
    18241 tagatatttt atccgttcaa aaatatctaa tataaattgt acattattac accaaataaa
    18301 ttatcttaaa atggtaattt atcaacatct tccaaaacta gattaactga attttctagt
    18361 ttttattttg ctatttcaag catatttgga tgtatatcaa ttccatacaa atattgttct
    18421 aaatttctgt tttcactaat ctaatttgca atcgttacag attacagatt agaaatagct
    18481 tttagactct acttatcttt atctacaaaa aatccttcaa taaattcagt aataatattc
    18541 agtaatctaa aaataaacct ctatctggat ttttaagata caagcctttt aggaagtatc
    18601 ttttgtgata atataaaaat tactcgtcat tatcatcaaa atcctcagca accaacattt
//
LOCUS       DAFKMX010000073        18641 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFKMX010000073 DAFKMX010000000
VERSION     DAFKMX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.073"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..321
                     /locus_tag="LZB08_001947"
     CDS             <1..321
                     /locus_tag="LZB08_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7315451.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="LZB08_001948"
     CDS             337..1920
                     /locus_tag="LZB08_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBM7315452.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="LZB08_001949"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="LZB08_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBM7315453.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="LZB08_001950"
     CDS             3475..4827
                     /locus_tag="LZB08_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBM7315454.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="LZB08_001951"
     CDS             5019..6128
                     /locus_tag="LZB08_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7315455.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="LZB08_001952"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="LZB08_001952"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBM7315456.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="LZB08_001953"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="LZB08_001953"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBM7315457.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="LZB08_001954"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="LZB08_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBM7315458.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12637..13659
                     /locus_tag="LZB08_001955"
     CDS             12637..13659
                     /locus_tag="LZB08_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBM7315459.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13719..14726
                     /gene="gap"
                     /locus_tag="LZB08_001956"
     CDS             13719..14726
                     /gene="gap"
                     /locus_tag="LZB08_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBM7315460.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14948..16150
                     /locus_tag="LZB08_001957"
     CDS             14948..16150
                     /locus_tag="LZB08_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBM7315461.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16176..16919
                     /locus_tag="LZB08_001958"
     CDS             16176..16919
                     /locus_tag="LZB08_001958"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBM7315462.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16928..18460
                     /locus_tag="LZB08_001959"
     CDS             16928..18460
                     /locus_tag="LZB08_001959"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBM7315463.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLREDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7461 a         2160 c         3763 g         5257 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcat ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttat catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacggtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt agaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacagaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacgaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatgggat ggaattttta aacccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggattaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaggct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagatact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggagcttgg gataaacgat ttcagcaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcgaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaatct
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcagca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaaaggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggctca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta ggacacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 aacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctagccta cttcctgaaa atatagtgca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagtcaac tctgttccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagct
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaacataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataataga atatggaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gaacataaaa aactaaagaa gattaaaaca ttaaagaaga
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
    12421 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
    12481 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
    12541 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
    12601 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
    12661 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
    12721 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
    12781 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
    12841 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
    12901 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
    12961 agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
    13021 agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
    13081 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
    13141 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
    13201 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
    13261 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
    13321 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
    13381 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
    13441 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
    13501 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
    13561 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
    13621 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
    13681 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
    13741 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
    13801 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
    13861 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
    13921 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
    13981 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
    14041 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
    14101 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
    14161 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
    14221 gtatagaaaa aggattaatg actactatac atgcatacac taatgaccaa aatactttag
    14281 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
    14341 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
    14401 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
    14461 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
    14521 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
    14581 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
    14641 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
    14701 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
    14761 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
    14821 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
    14881 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
    14941 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
    15001 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
    15061 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
    15121 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
    15181 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
    15241 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
    15301 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
    15361 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
    15421 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
    15481 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
    15541 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
    15601 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
    15661 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
    15721 agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
    15781 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
    15841 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
    15901 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
    15961 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
    16021 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
    16081 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
    16141 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
    16201 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
    16261 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
    16321 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
    16381 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
    16441 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
    16501 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
    16561 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
    16621 ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat gagccaatct
    16681 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
    16741 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
    16801 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
    16861 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
    16921 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
    16981 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
    17041 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
    17101 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
    17161 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata aagttttatg
    17221 tgaagcaatg gataatgcaa aagaaaactc tcttcatgta atgggacttt tgtctgatgg
    17281 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
    17341 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
    17401 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
    17461 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
    17521 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
    17581 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
    17641 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
    17701 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
    17761 aagagaagat attaaaaact tctttgtatg tttaacagag tacgatataa ctatagagaa
    17821 tgtgcatata gcatttggac ctcaatcctt agccaacact ctaggtgagt atctagctaa
    17881 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
    17941 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
    18001 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
    18061 tttagataaa ttaagagaag ataagtttga ctttatagta ttaaactttg caaatcctga
    18121 tatggtaggt catacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
    18181 ttgtgtagga aaattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
    18241 tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg taacggctca
    18301 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
    18361 ggatggagga agattatctg atatagcacc gactgtttta gatatgatga agttagaaaa
    18421 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagaga
    18481 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
    18541 tttagacttt atgacataag atttagatat tgtaaggtgt agatgaagat atattttata
    18601 aaatttacaa gatagacaat ttataaaata aaaaattaca a
//
LOCUS       DAFKMX010000074        18587 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFKMX010000074 DAFKMX010000000
VERSION     DAFKMX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.074"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            1..1893
                     /locus_tag="LZB08_001960"
     CDS             1..1893
                     /locus_tag="LZB08_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315464.1"
                     /translation="MKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLKEGIPLAE
                     KSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEPFGIIGFN
                     NVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAHIVKRLNC
                     KGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVDKNFKITL
                     FNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTKIITNRVP
                     IIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHTSEKTKDCIEL
                     AKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSLIESEFFG
                     YEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQVMRIGGNK
                     VIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDIIKLSEFFI
                     HKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSILLEKVSNE
                     YTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRSTLWRKLK
                     EIEDDV"
     gene            2164..3138
                     /locus_tag="LZB08_001961"
     CDS             2164..3138
                     /locus_tag="LZB08_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBM7315465.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            3159..3434
                     /locus_tag="LZB08_001962"
     CDS             3159..3434
                     /locus_tag="LZB08_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBM7315466.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            3447..4610
                     /locus_tag="LZB08_001963"
     CDS             3447..4610
                     /locus_tag="LZB08_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBM7315467.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            4947..7226
                     /locus_tag="LZB08_001964"
     CDS             4947..7226
                     /locus_tag="LZB08_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7315468.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(7321..8661)
                     /locus_tag="LZB08_001965"
     CDS             complement(7321..8661)
                     /locus_tag="LZB08_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7315469.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(8673..9455)
                     /locus_tag="LZB08_001966"
     CDS             complement(8673..9455)
                     /locus_tag="LZB08_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315470.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(9470..10492)
                     /locus_tag="LZB08_001967"
     CDS             complement(9470..10492)
                     /locus_tag="LZB08_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBM7315471.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(10498..11511)
                     /locus_tag="LZB08_001968"
     CDS             complement(10498..11511)
                     /locus_tag="LZB08_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBM7315472.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(11639..12577)
                     /locus_tag="LZB08_001969"
     CDS             complement(11639..12577)
                     /locus_tag="LZB08_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315473.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDATPFFSQSYNPIGKELLLESVKNELTK"
     gene            12847..13311
                     /locus_tag="LZB08_001970"
     CDS             12847..13311
                     /locus_tag="LZB08_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315474.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQSKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(13423..13740)
                     /locus_tag="LZB08_001971"
     CDS             complement(13423..13740)
                     /locus_tag="LZB08_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7315475.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(13753..14484)
                     /gene="chbG"
                     /locus_tag="LZB08_001972"
     CDS             complement(13753..14484)
                     /gene="chbG"
                     /locus_tag="LZB08_001972"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBM7315476.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKSVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(14510..15820)
                     /locus_tag="LZB08_001973"
     CDS             complement(14510..15820)
                     /locus_tag="LZB08_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBM7315477.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(15917..17230)
                     /locus_tag="LZB08_001974"
     CDS             complement(15917..17230)
                     /locus_tag="LZB08_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315478.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(17252..17563)
                     /locus_tag="LZB08_001975"
     CDS             complement(17252..17563)
                     /locus_tag="LZB08_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315479.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(17763..18467)
                     /locus_tag="LZB08_001976"
     CDS             complement(17763..18467)
                     /locus_tag="LZB08_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7315480.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
BASE COUNT         6552 a         3044 c         2482 g         6509 t
ORIGIN      
        1 atgaaggaaa tactcgttat tgctcccact aaaggaacct atgaaaaatc aattcacata
       61 gttaaaaaaa acaaatatac aaatattgat gttgtatttg gcaatctaaa agaaggtata
      121 cctcttgctg aaaaaagtat taatcatggt actagaatta tcataagccg aggtggaact
      181 tacaatatgt taaaagctac ttacaatata cctatagttg aaattaaggt agatgcttat
      241 gatattataa aaagctacaa ggaggtaaaa aattccaacg aaccatttgg aataattgga
      301 tttaataatg tcatctacgg tttcgatatt atagaagaaa tattgaataa gaaaataact
      361 atgattgaaa ttgaaaaaga agaagaaatt tatgatgcta tagaaaagta cagaaagaaa
      421 ggtataaaca catatatagg agataccact gttgcacata ttgttaagag acttaattgt
      481 aagggaatat taatcgaatc tagagaagaa aacatactta gagccattca acaagcagaa
      541 caaatactcg aagcaaccaa ggatgaacaa aaaagaagat tacagataga agctatgaca
      601 gattttgtac atgatggaat aataacagtt gataaaaatt ttaaaataac attatttaat
      661 aagagtgctg aaaaaatatt tggtataaaa aaagaaaatg cactttatca taatgttgta
      721 gatgttatcc caaacacagt tcttccacaa gtagttgaga ctatggaacc tcaaattggt
      781 gaaatacaaa atatagggag tacaaaaatt attacaaata gagttccaat cattgttgat
      841 ggtcagattc aaggggctgt ttcaacattt caagatagca aggaaataag ttcttttgaa
      901 catacaataa gaaggagctt attaagcaaa ggtctatctg ctaaatacac ttttgatgat
      961 ataattcata ctagtgaaaa gacaaaagat tgtatagaac ttgcaaaaaa atatgcttgt
     1021 tatgatactc ctatgattat tactggagaa tctggagttg gtaaagaact attttgtcaa
     1081 agtatacata actatagtaa gcgtaaaaat gcaccttttg ttgctgtaaa ttgtgctgct
     1141 atacctccat ctctaataga gagtgagttt tttggttatg aggaaggttc atttacaggt
     1201 gctagaaaaa aaggtaaagc aggtgtattc gaactagcac atgaaggtac aatcttccta
     1261 gatgaaataa gtgaaatccc tcttgaactt caaggtcgat tattgagggt attgcaggaa
     1321 aaacaagtta tgagaattgg tggtaataaa gttattccta tagatgtaaa aattatctgt
     1381 gcttcaaata aagacttaaa aaatatgatg cgcaatggtt tatttagaca agacctttat
     1441 tttagaataa atatactaac attatatctt ccttcattaa aagaaagaaa agaagatatt
     1501 ataaaattat cagaattttt tatacataaa tattcaagca aatacaataa aacgccttta
     1561 attatcactc caaatattaa aaaggcactt cttgaacgag aatatgaagg taatataagg
     1621 gaattggaag gtatgatgga aagggctgtt attgtggaat catttgactc gattttatta
     1681 gaaaaggtta gcaatgaata tacaaataat atagttgata aagataatag tgattttgaa
     1741 tgcagtaatt tagatttaaa aactttagaa aaaaaatata tctataaaat ttataacgaa
     1801 aataatagaa atactacaaa aacctgtgaa attctaaaga taactcgctc cactctatgg
     1861 cgtaagttaa aagaaataga agatgatgtt taaaaataat acatatctat atatatcaaa
     1921 acgttttatg tttaatttta atacataaga cgtttatttg ttttcaataa aaaatacaat
     1981 tatatgataa aaagaatctt tgtaagtgaa attctcgtca atcgtacttt taattataag
     2041 taatctacag catttcaatc gattattaaa aataatcatg ataactaaag atacaaactc
     2101 tatatattgg catattaatt gcttttataa tacctatata aaaatgaaag ggagtgatac
     2161 tagatgcaca tcttaaaatc agtgaaaaag ataccaggag gacttatggt ctgtcccttg
     2221 ttattgggag cactatttaa tacagtatgt ccacaagctc ttgaaattgg aggattcaca
     2281 acatccttat ttaaaactgg tgcaatgagt atacttgctc tattttgtct atgcaatgga
     2341 gctcaaatca atatcagaca agctggtaaa ccattgacta aaggtatagt acttacagca
     2401 acaaaattta taattggtgc agtattaggt attattgtta gtaaatttat tggacctaaa
     2461 ggtataattg gtattacacc tcttgccata gttgctacca tgactaattc aaatggaggt
     2521 ctatttgctg ctcttgcagg tgaatatgga gactccacag atgttggagc aatatctatc
     2581 ttatctctaa atgatggtcc tttttttaca atgcttgctt ttggagtaac tggacttgca
     2641 aatgtaccta taatagcact atttgcagca cttataccaa tactacttgg atttatactt
     2701 ggtaaccttg atgaagatat acgagaattt cttgcacctg gtacaacatt attaattcct
     2761 ttcttcgctt ttcctttagg agcagcatta aactttaatc aaattataac tgctggacta
     2821 cctggagttg tacttggttt aggagttact ataataacag gtatgggtgg gtactttgta
     2881 atgaaactta tacgtgcaga acatccagca gtaggtgcag ctacaggtac aacagccgga
     2941 aatgcagctg gtactcctga agctttagca gctatagacc ctactctagc tacaatagca
     3001 gctgtttcaa cagtacaggt tgctgctgct attattgtta cagcaatatg ttgtccaatg
     3061 ttagtttcct atctagataa aagagaaaaa aagaaaaaat ctatttttaa aacaaataaa
     3121 aatattgaag taaattaata cttaaggagg taaaattaat gaatgcaatt attcatgcaa
     3181 atgaaaaaga taatctagtt acatgtgtca gacctttagt taaaggtgaa aaagtttcaa
     3241 ttgatggaaa atcttacata gtaaatgata atataccggt ctttcataag atggctacag
     3301 aagatgtaaa aaaaggtgat gttgtataca aatacggtga aattatagga attgcaacag
     3361 ttgacataaa aactggtgac tatgttcatg tacataacat tgaaagtaca cgtggtcgtg
     3421 gagataaaaa ataggaggaa gttaaaatga aattaatggg ttatttaaga gaaaatggtc
     3481 agtttggtat tcgtaatcat gttttaatta ttccaacatc tgtatgttca agtgagacag
     3541 ctacaagaat agcatcactt gtgcctggag caatagcaat ccctcatcaa catggatgtt
     3601 gtcaaattgg ttctgatata gagcttacag caaagacatt aataggattt ggaaaaaatc
     3661 ctaatgttgc cgcagtttta gtagttggtc ttggatgtga tggtattcaa gcaaaagaat
     3721 tagcatctga aatagctaca acaggaaaaa aggtagatta cgttgttata caggaatgtg
     3781 gcggaacatt aaaaacagtt tctaagggag ctgaaatagt aagtaaaatg gctcgtgaag
     3841 tatcaaaaga agtaagagtt gaatttggta tgagtgaaat tacattagca cttgaatgtg
     3901 gtggttctga cccaacaagt ggaatagctt caaacccttc aataggcgtt gcatcaaacc
     3961 ttcttgtaga tgaaggtgga agtagcatac taagtgaaac tacagaagta attggtgcag
     4021 aacatttgtt ggctacccgt tttgaagatg aagaaatgaa agataagttt cttaaatttg
     4081 taagtgatgt agaaaaaaga gcaattgcta tgggagaaga tttacgtagt ggtcaaccta
     4141 ctccaggcaa taaagctggt ggactttcaa caattgaaga aaagtcactt gggtgtatgt
     4201 ataaggcagg caataagcca ttcaaaggtg ctcttgaata tgctgatatt gtacctcctg
     4261 ataaaaaagg attatatttt atggatactc caggtcaaga tatagattct atcactggta
     4321 tggtagcagg tggtgcacaa attgtaatct tttcaactgg tagaggtact ccaacaggaa
     4381 gccctatttc accagttatt aaaatcactg ggaatagtga tacatacaac aaaatgccag
     4441 acaatatcga tataaatgca ggtagaatta taacagatgg tgctaaaata gcagatatag
     4501 gtcaagaaat atttaatgaa ataatagaag tctgtaatgg taaacatacc aaagctgaaa
     4561 gtcttggtca tagagaattt ggcatttaca gaatttcatc tacattttag attgtaacaa
     4621 aatatctttt ataaatataa tacttatgta gatacataag ctaaaagttc tttctaatac
     4681 tataaaaatt ttctatactg aaaaggaact gtatcaaaat tattttgaaa acagttcctt
     4741 ttttaacctc ttttactcaa aatattataa aataagattt aaacaataaa cgtatcttga
     4801 ttaatctcta agaagacata aataaagcta aatgtaaagt gggataaata aaatttaata
     4861 tctttgtatg aaaagatatt tggtataata tgtctgagag aattcttatt tgtaaacaaa
     4921 taaaggatgg aggatgaaac aaaagaatga actttgaaaa cacaaaaaat aaaaaaataa
     4981 tagctacttt actattgaca ctccttttaa tcgtgtcatt aacatgtgtc acttttatta
     5041 tgaatctaaa taaagtaaca cataatgaaa cagaacgata tcttgtagaa gtttcacaag
     5101 gtatttccac tacaattaat gtgaaatgta aaagtatatt taggacacta tattctatgg
     5161 gagatactta ttttgagatt aatgatagtg aaaaagaagt caatagatac ttatcaaaaa
     5221 aattaaaatt atttgaattt gattggttag gtattataga ttctaatgga aattttgtaa
     5281 gtgcccttaa agatacaggt aatgttaaag gacaagactt ttatagaaaa gcccttcaag
     5341 gcaaagaaac aattactcct gttaaatcac aatctggtac atatggaatt ttatattctg
     5401 taccactata caatggagat aaaattgttg gagctgttgc tgcttggaat actattgata
     5461 ctatgagaaa tttattaagt gtagaaagct ttcatggtga aggattttcc caaattatcg
     5521 accaagatgg aaattttatt gtaagttcag caaacaaaaa tgctccaaaa aatgtggtaa
     5581 atttttttga catgattaaa caccagggaa aacaggttga taaaaataaa cttgaagaaa
     5641 tgaaaataaa cttaaaaaag aacaaaactg gttctatgta ttatacatta aatgatggta
     5701 tttctaaagc aatgcactat cttccattac aagaagaaaa cttatacctc ctttcaattg
     5761 ttccaacaaa aacagcaagt gcacagtttg atttacttct aaaaaaagca atcttcatca
     5821 atattttaat tgttatacta tttttattgc ttattatatt gattatgttt ataaatcaaa
     5881 agaacagggc acaacttata aaaatcgcct atgtagactc tgtaactaaa ggttacagta
     5941 aagcacgttt tgatattgaa gctaaaaaaa ttatacagtc atcttcatca gaaagctatt
     6001 cattggtatc tataaatatt cagaaattca aacttattaa tgattctttt ggtagtgaag
     6061 caggagataa aacattaaag tacatacatg atgtcattag taattatcta gaaccaggag
     6121 aattgctttg tcgagtttct aatgaccagt ttaatgtact tattaaaact attcctcaaa
     6181 aagaaattct taagtacata gaaaatatca ccaatgatat taatagtttc aataaaaatg
     6241 aagaatgtaa atattttata gctctatcta taggtgtata taaaattgat gacccaaatc
     6301 tacctttagt ttcaattcaa gaccgtgcaa atgttgcacg aaaaaataat aaaggaaaca
     6361 ttggaaatca tccactttat acatgtgttt tttatagtga tttagagcgt ttaaaaatga
     6421 ttaaagaaaa agaaatggaa aatagaatgg aggaagcatt aaataataac gaatttatag
     6481 tatatcttca gcctaaaata agcctcaaag ataatactat tgtaggtgca gaagcattag
     6541 taagatggca agacccaaat aaaggtctta taccaccaga cgagtttatt cctttctttg
     6601 aaaaaaatgg ttttattaca aaactcgacc actatgtttt tgaaaaagta tgcagtatga
     6661 taagaaaatg gatagataat ggagaaactc caatacctgt ttcagttaat ttttcacgtg
     6721 ctaatttaaa taatatagat tttttagaaa aatacagaat gcttcgtaat aaatacaaca
     6781 ttcctgctaa gctcttagaa attgaattaa ctgaaacact tgtatttgaa aatctccaaa
     6841 aattaattaa tataatagac caaatccatg aagaaggttt tcaatgttca ctagatgact
     6901 ttggaagtgg atactcttca ctcaatatgt taaaagaaat aaaagttgac acattaaaac
     6961 ttgaccgtgc tttttttagc tcacctaatg cagataatat tgctgaaaat catgtaatag
     7021 aatctatagt taatttggca aaaaaattaa atatgaatag tatatcagaa ggtgttgaaa
     7081 caatcttgca aatggattat cttaaaaaaa ttaattgtga tatgatacaa ggatatgttt
     7141 tttcaaaacc tctacctcaa gaaatatttg agaaaatgac ttttggaaaa aaactagaag
     7201 aaaccagtat ggatagttcc gtttaaataa aataaaaaac ttgtctctat aaaataattt
     7261 taatcatttt ataaagacaa gtctttttat tttccaattt aattttattt tctaaatttc
     7321 ttacactcgg cttgttttaa attccctttt taagaataat aatacgatta tcatagcaat
     7381 aaaatctgca actggctgtg ccaaccaaac accatctaaa ttaaattgaa ttggaagtac
     7441 aatgattaat ggtataaata aaataaattg tcttaaaaga gttaaaaaca ttgaatgttt
     7501 aactttactt atacactgaa aatacacagg tcctacaata gaaatcccca ttataggcaa
     7561 tgtaattaga tttaggctta tacctcttac tgctatatcc atcaaatctg aatctttatt
     7621 aaatatacct acaaatactt ctgggaaaac ttgtactact aaaaatccaa atgacaatat
     7681 gacaactgac actatgatag ataatattaa agttttcttt gcacgctcat attgttttgc
     7741 accataattg tatgatatta tagtttgcat accttgattc aatccaaaca caggcatcat
     7801 aaacatcaat aaaatagatg taagtgctgt catagctcca atagctaaat ctccaccata
     7861 aacctttaga gaattatttg taaacatgtg agttatactt atagctactt ccatagcaaa
     7921 aggtgtcatt cctattgcaa ataccttctt tactatatct ttattcaatt ttaagttaca
     7981 tttttttagt tttaaattgg acttccctct tataaaataa tgtattaccc aaaagaatac
     8041 tactatttga cagagtactg ttgctatagc tgctcctttt atacctaaat taaatacaaa
     8101 tataaatata gggtccaata tcaggtttaa tacacatcct acaatcattg tccttgctgc
     8161 taattttgga ttaccttctg ccctaattgc attattaaga gaaaatgcaa ctaaattaaa
     8221 tatacttcca accaatatga cactcatata gtcttttgca taaaatattg aatctttact
     8281 tgcacccaaa ataaaaagta tatcttctaa gaaaactaat cctaatatag ttataattaa
     8341 agagataatt atagataatg tcaaagcatt acctaatatc ttctctgctt cttctctatt
     8401 tctttcacct aactttatag acaatcttgt gctagcacct actgatacca acattccaaa
     8461 agctattatt agagtaaata ctggcatagt tacaccaaga cctgcaatag ctatagagcc
     8521 aataccttcc atagaaccaa taaaagctct atctactaca ttataaagtg aagttaccat
     8581 cattgctatt attgcaggta tcgaatacct aaataataaa atccatattt tttcatgtct
     8641 taatgcttct tgattctcca tgttgtcacc atttacacca tgtcataagt tatacaaatt
     8701 ggtttatttg ttcttgggtc tgttgctatt tctgcatcaa tattaaataa ttcttttaga
     8761 ttatccttcg tcataacatc atatgcatca ccttcacaaa ctattttacc tttcttaata
     8821 cctatcatgt ggtcagcaaa tcttgctgca ttattaagtt catgtattac catgacaata
     8881 gttcttcctt gttttttatt tagttcatct aataatttta gtatctccaa ttgatgtgcc
     8941 aaatctaaat atgttgtagg ctcatccagt aataatatat cagtttcttg tgccaaagcc
     9001 atagctatcc atgccctttg tctttgtcct cctgataaat cttccatatt tctatcctta
     9061 aattctgcaa ttccagtaac ttctaaactc caatttacaa tctctctgtc ttctttacta
     9121 gaattaccaa atccactttt atgaggaaat cttccataag aaattaattc tgaaacggta
     9181 agaccactag gagcttgagg gctttgtgga agtacagcca tatcttttgc tatttcttta
     9241 gacttctttt tgtagatgtc ctctccattt atatatattt tcccactatt agggctaata
     9301 attctaccta tagttttaag tattgtcgat tttccacaac cattagcccc tatgatggta
     9361 gttatttttc cttttggtat atttaaattt aaatttttta taatatctgt gtttccatat
     9421 gatacattta gattcttagt atttatacag ttcataacaa caccctttcc taatcattta
     9481 ccaacattaa ataaataaag tacggcactc ctattataga aactactata cctactggta
     9541 tttctatagg tgctagaaga tttcttgata tagtatctgc tattaaaagt aataatgttc
     9601 ctatcaatgc tgatactggt attaatcttt tatgctttgg tcctattatt tttctaccta
     9661 tatgtggagc tattaaacct aaaaatgaaa tactacctgc tactgctgtc gatacaccag
     9721 ataaaattac tgcatatatt ataagtcttc ttctttgttt ttctacatct acaccaagac
     9781 ttatagatac ttcatcaccc aaatttaaaa catctatata tttagattta taaatagtta
     9841 tacccataaa aatcactata aatggaagca cagctattac atatttccaa ctggttcccc
     9901 aaatgcttcc tgatgtccat gccataaccc tattaaattc ctgtgttgta aatttcaatt
     9961 ggaatattgt caaaattgaa gtaaaagcta tgtttacacc tataccaata agtagtaatc
    10021 ttgacgaatt tataccacct ttccatgcaa gagaatatat aagaaatgct ccaaataaag
    10081 ctcctataag tgcaacaata ggcattgtaa atacagtcaa gttactcata ccttcatata
    10141 cattaccatt cataaggtat atataaacta ctacaaacaa tgcagcacct gaatttattc
    10201 ccaaaattcc agaatcagct aaatcattct ttgtaactcc ttgaagtatt acacctgctg
    10261 ttgataatgc agaaccaacc aaaagtgcta ttactattct tggtaatctt aatttaaata
    10321 ttgcaatttc atgattacgc tgaccctgcc ctaaaaaagt ctttataaca tccattggtt
    10381 ctatagaaaa tgagcccata tttaaactca ataaaaaagt gataaaaata agcaccatta
    10441 aaattgatat aacaagtaat gttttagaat ttatttttat aatatttttc atagtaccta
    10501 tttctccccc tttctaacaa ggtatataaa tattggcact cctataagtg ctgttataga
    10561 acctactggt gtctcatatg gaggatttat catcctactt aatatatctg tatatacaag
    10621 caatacagct cctaatacta aagagctcgg tattatatac ttataatctg aacctactat
    10681 tcctttagct atttgaggaa ctataagacc tataaaaata atattaccag acactgacac
    10741 agatgctcca gtcattaata ttacaagtac tatacatata aatctaacaa agttagtttt
    10801 ttgtcctaat cctattgcca cttcttctcc aagacttaat atagttattc ttggagccat
    10861 aataatagct aaaataaatc caataccacc agctacaaat agcaatttta taccttccca
    10921 cttcactcct acaagccctc ctgatatcca gaaacttaat tgttgagata ggttaaaata
    10981 catagatatt gctgatgcca aagaaattag caatgtacca agagcgactc ctgcaagagc
    11041 taattttaca ttattaactc ttcctcttga tttagaactt ataaaatata taaataatcc
    11101 acttatactt gctccaataa atgcaaacat cattacacta aaacttccat gtataagttg
    11161 aggaagtttt ttttgaagta ccagtgatat cgaaattgcg aacgtagctc cttgtgtaat
    11221 tcctataaca gatggttcag ctataggatt tctcgttatt ccttgcatta ttgccccaga
    11281 aactgctaaa aaccctccaa caagtgcagc tgctattgcc cttggaagtc taacatcctt
    11341 tactattctc atatttattc catcttccat ttttatcaaa ctatctataa cagttgatag
    11401 attcatatct tttgccccaa tagttatgga taatacaatt ccaaatacaa gtacaactat
    11461 acttactaat atcaacaaat aagttatatt ttttttactc agcttagtca attatcctac
    11521 ctccttttcc cttttaatcg tcagtataat atcatctatt aagtatttta attttaagat
    11581 ttattaaaaa taggtgtgaa aaatgaatat cattatctca cacctactaa gttaagaatt
    11641 actttgttaa ttcattttta actgattcta ataataactc ttttccaatt ggattgtatg
    11701 attgactgaa aaatggagta gcatcaagta ttgttacatt accatctttt actgctctta
    11761 tttgagccca gatagcacta ttttctaaat cttttttatc tgcttctgta gcaattacaa
    11821 ctatatggtc tgcatctata tctgttaaac cttccatact taccattggt agtgttattc
    11881 catcctgttt tggcatattc ttaggtctag ctaatttcat atcatcattg attagagtac
    11941 ctattccacc atcactaaat accataaatt gacctgaact tgccaacact ggtaaataag
    12001 ttttttctcc attcttttct ataacttcct tacctaactt tgtagctttt tcatcatact
    12061 tctgtagcca acttttagct tcttcttctt tgtcaaataa ctttgaaaca tcagttaact
    12121 tgcttctcca gtcatttgca taatctttca tcataactac aggagctatt tcttttaatt
    12181 ggtcatatat tttttcttgt ctttgactca ttattattaa atcaggattt acttctaata
    12241 tagcttccat atccatagta tccatcattg agtgcccaac tatctttaca cctttaagct
    12301 cttctcttat gtaacttggt aatttatcag tttcatatga gtctacattt gctgttgcaa
    12361 caggcttata acctgctaat aatagttctt cactacttcc tgatatatca actatttttt
    12421 taggatttga tggtatctta acctctcctt taacagattg tacaactctt gtctctgatt
    12481 tatctttatc ctctgtattc ttatctgaac atgctgtaac aagtactaac gtagttataa
    12541 tagatataaa tattgcaagt gattttattt ttttcatgat tacttctcct acttgtttat
    12601 tatacttata tgatattgat tatcataagc acatatttat tatagcattt ttctattttt
    12661 agtcaatatt attttgtaaa aggataaaaa ttcctaatta atcttaatta tatatagctt
    12721 agataaggtt ttcttatatt aaacagtatt ttttttaaga attggacaca aatacaatga
    12781 cttttcagtt aaacaatgat aaaatgcaat ttatagataa ggaatttagt agggaggatt
    12841 caaaacatgg tacatttagt atattgtgat aatgcaggaa aaaaaggaga aaaggtctta
    12901 gataaaatct tggctggaac aaaaacaatg gttattagag gtgctgctgg tagaaaaata
    12961 ccacatagta gagtatttga agatgagtta ctttatttta tggaaaaagg aactgcaaaa
    13021 atatctgcaa aagccactgt taaaaaggta gaaaactatg ttaaattaac agaagatgaa
    13081 atttctaaaa ttttaaatga aaatcaatca aaattaaatt tatctgaaaa gcaaaaagtt
    13141 cgttggcata aaaaatgttt atgcttagtt gaatttgaaa atgttgaaga aataacacct
    13201 cttgattttg accatcaagg aaatatggat gattggctta ttgttgaaaa aattgaagat
    13261 gtagttgttg gtactagtat accttacaat tatgagaagt ctaaatttta aagtatcaat
    13321 aacataatac tatattgcta aactctactt aaataaaaaa agagtgcatt taataattat
    13381 aaaaattatt tttatacact cttttttaat aataatttta aattacttac tttctaatct
    13441 ttcatataca tctataaatt cttctgctaa ttgttgaaaa ttcatagctg tcattaaatg
    13501 gtcttgtgca tgaataagta taacacttat ttcagtttta ttaccattag cttcattttg
    13561 tatcaattct gtttggaatt tatgagcttt atgaattgat tctttcgact tttcaataca
    13621 ttctctagct ttatctatat tcccgctctt tgcttctttt attgcttcaa atgctagccc
    13681 tttcgcatca cctgcaaatc caattattcc aaaagatatc tctatcatct tctcatccat
    13741 ttattcgacc tcctaaatat ctctatagct tgatatgact aatccattct tttctaaaaa
    13801 ttctttgact tttttagatg taagtatctt gtgctctttc gttctatcaa tagcataaga
    13861 tgtagaattt aaaatataat catctaaaaa agctgggtga gacataatat ccactacatc
    13921 atacttcatt atttcgtcta tattttttat aaaatattct tcacttacat tttccttata
    13981 aaaactatct attaaaggaa ctactttaga atactctaaa ttatattcaa atccacctct
    14041 tattggaaga ttatacttgg ttacaatctt ctctattact gactttaaac ttactaaagt
    14101 atggatatga tggtgactat ctaaatgaga tatatctatt ccaagccctt ttaccctatc
    14161 tatttgagca caaagttctc tataaatctc atcacaatcc attttttcaa taatttcgtc
    14221 tgtaatcctc ctaataaaaa aaccgtcttt atcaacaata gtttttaagt tagataataa
    14281 tggtctacca caacttaaag tcatatgaac tccacaattt aaagttttat tttctttaag
    14341 taagcctact ccatgttcta ccccaggcat atttgccatc atagaagtag acctaactat
    14401 accattatta tgagctgaaa ttattccata gttcacagct tcacaatatc caaaatcatc
    14461 tgcatttatt ataattttag tcataccata acctactttc tatagacaac tatctattaa
    14521 atctaggaag ataatcttta tgagcttcca acatttcgtc taatatagtt tttgctaagt
    14581 catcagatgg aatcaatggg tttatacaaa gtgccaaaag agcagattca taatcgccat
    14641 atacagctgc tttagctgcc aatacttcaa atgatttgat ttgacttact aatccatgca
    14701 cagaatctgg taaatatcct atacttattg gttttggacc attttttgtt attacactac
    14761 ttacttctac tgcttgttca tctttaaaat ctcttataga acccttattt aatgtattaa
    14821 ctacttggat atcttttttg tcattgtata tagaacttat taaattacaa gcagcatcac
    14881 tatagtaagc ccctcctctt ttttcaagtt gaggtggttt tacatctaat ttttcatcct
    14941 tatataattc aaataattgt tcttccaatt ctttaactac ttgacctcta gcttttccct
    15001 ctttaaactc tctcaactca tcctcaagca tttctttagt tttatagtag tatctatggt
    15061 atggacaagg tattgcaccc aaagatttta caaattcagc attaaaatca attgccttta
    15121 tattttgcat acttatatct gctttgacaa atttatcaat tgcttctttt gtaacatctt
    15181 ctccatctaa agatacattt agaccataaa ccatatgatt aagacctgca aaatccatac
    15241 ttattctgtc actttctaca ttaaatagtt tggcaacatc attttttaaa tgaattggca
    15301 cgttgcatag acctatatat cttctaaaat tagtatattt gaatacagcc tctgttatta
    15361 ttcctgttgg atttgtaaaa ttcaccatcc atgcatttgg acataattct tccacatctt
    15421 ttattatatc aaatataact ggtattgtgc gaagggcttt aaataaacct ccagcaccat
    15481 tagtttcctg ccccataact ccatgagata aaggaatagt ttcatctttt attctcgcat
    15541 ctaaaagacc tactctaagt tgtgttgtca caaagtctgc atctcttaga gcttctcttc
    15601 tatctaaagt aagatttatt ttcatatcta ctccagcttt ttttaccatc ctcttagcta
    15661 gatttccaac tatctctaat ttttcttttc cagcttctat atctacaagc cataattcct
    15721 ttacaggtaa ctcttcatat ctatttataa aaccctctac aagctcagga gtataacttg
    15781 aacctcctcc aattgtaact atttttagtc cctttttcat ctcattatac catccttacc
    15841 tataatttaa tatactctac aaaaccccat acatttattt aaaactctat acatttattt
    15901 accgaaataa aactacctag ttgttaattt tcaatttatt agcttccatt ttttcagacg
    15961 ctatgacaaa tggaatatat attaatattg ataatattaa attaaatagt gctaaagctc
    16021 cgccagtcca gtcacctgta gccattgctc cacctaatat aggaggtgtt acccaaggta
    16081 ttttaggtaa aattactggt gctactaatc caaaatctat tgctaagtat gatacagcac
    16141 tacatattac tggtgcaagt acaaatggaa tcataaatat aggatttaaa actattggaa
    16201 gtccaaatat caatggttct cctatgttaa atattgcagg tggaccacct aatactatca
    16261 tctctttacg tcttcttcca gctattaata aagctactat taatcccaat accattccag
    16321 aaccacctaa atatacaaat gcatcaaaga atgcacctgc aagtacatgg tatccttccg
    16381 ttgctcctgc ttgagccaag gcagcatttt ctccacctaa tttcataagt atagtttctg
    16441 taaaaggaag tgctatattt gcaccatgaa gtccaacagt ccaaagtata tgaattataa
    16501 atgcaataag cattgtagaa cctagactat cagctacatt tgatagtggt gctcctaaat
    16561 aagtatttaa aatatctgtt aagaaacttc cattagaaag catttttata aatattccta
    16621 ttacagtaaa tatcattata gttaacattc ctggtattaa ctttgcaaat gatcttgaaa
    16681 ctgctggtgg aactccatct ggcattttta tagttaattt ctttacctta gataatctta
    16741 caaatatctc agttgataat aatcctatta atatagaaga gaataaagct gctgtaccta
    16801 agtaggtttg ttgtatcatt ccccatcctt caactactgt agttcctccc tcaggaacta
    16861 ttttaccaat ttgtggagcc attataaaaa agatagataa agctataagc ccagattgta
    16921 acccatcact ctcataagat tttgctagaa aataagcaac acctattact aagaatactg
    16981 ccattgtacc tattgctccc caccaaaggt cgcctccaaa tgtggtccag ttttctccaa
    17041 atatacttgc catcaaattt ttgtatgctt ccaatggtaa gttatttatc aatgttccca
    17101 atgcacctac catagttata ggtatcatta ctataaatgc atctcttact gctactaagt
    17161 gcctttgagc tcctatttta gcagctacag gcactatata tttatccata aatgacatga
    17221 atttttccat tttacatcct ccagtacaac tttattttga actatttaac tctacagctc
    17281 tatctaacac agcttttcca ttacatcttc cataatccat catatttata acttctactg
    17341 gaatattata tggttttgcc atttcagttg cttttcctaa tacatatcta acttgaggcc
    17401 ctaaaagtaa gacatcacaa ttttctattt cattttttaa gttcacttct gatatagccc
    17461 atatttttgc ttctataccg ttttcctttg ctgcatcctc catttttgta actaataaac
    17521 tagttgacat tcctgctgaa caagctaaca ttatttttat cattttttcc cctccaaatt
    17581 agattcatta aatatttttt aagtcataag ttttttaaac tatacattta aaatatgctt
    17641 ttctattaat tttttcttaa tgtatatatc ttatttttaa atgtatatac atttaattct
    17701 tgatattatt atataccaaa tgtatataca tttcaagaaa actttttcat tttatttaaa
    17761 ttttattcga caacagcatt caaagttaat gtatatccat agtgatatgt gtgtgagtac
    17821 tcaaaaatct cactattttg taacttacaa atttggctta tatataaaag tggctctggt
    17881 tcttctaatt ttagattatc catacactct tcattcgata ttacagcttg tacttctctc
    17941 tctgacatgg ctatcttgta tccacacact tcttcaacaa aattataaag agaattttca
    18001 caatgagtct tatttaaatt tggaaataac ttaactggca tataagtaat catatatgaa
    18061 actcttgcat cattggctaa tcttactctt tttatttccc aaacattttc ttctaaactt
    18121 atattcaact tacttgataa attttcatct gcctgaacta tttcaagaga taccaatata
    18181 ttagatactt tatagccttt actttccatc tctttagtaa atccacttac agacgatata
    18241 ttatcatata tattatttgc aataactata ctacctacac ctctttgtct agttatatag
    18301 ccttctttaa ctagattatt aagtgcagac cttacagtca ttctcgttac attaaattct
    18361 tcactcagct gtttttcaga agggattaaa tccccttctt tcaaactgtc tgaatttatt
    18421 aactctctaa catgattttc tataacttta taaattggct ctttcattta attcactcct
    18481 tattaaatct tttatataat aagataattt tatatacaaa caaagaaaat gtctatacat
    18541 ttaagataat ttaaattttc ttaatataga ttataattta catatgt
//
LOCUS       DAFKMX010000075        18417 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFKMX010000075 DAFKMX010000000
VERSION     DAFKMX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.075"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            108..1268
                     /locus_tag="LZB08_001977"
     CDS             108..1268
                     /locus_tag="LZB08_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBM7315481.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1610..1771
                     /locus_tag="LZB08_001978"
     CDS             1610..1771
                     /locus_tag="LZB08_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315482.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1964..2593
                     /locus_tag="LZB08_001979"
     CDS             1964..2593
                     /locus_tag="LZB08_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBM7315483.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2832..4091
                     /locus_tag="LZB08_001980"
     CDS             2832..4091
                     /locus_tag="LZB08_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBM7315484.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4349..5344
                     /locus_tag="LZB08_001981"
     CDS             4349..5344
                     /locus_tag="LZB08_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBM7315485.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKIHKKLVVAGLAIVMISMIIISSII"
     gene            5590..6657
                     /locus_tag="LZB08_001982"
     CDS             5590..6657
                     /locus_tag="LZB08_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBM7315486.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7191..9101
                     /locus_tag="LZB08_001983"
     CDS             7191..9101
                     /locus_tag="LZB08_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBM7315487.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9164..9787)
                     /gene="ytaF"
                     /locus_tag="LZB08_001984"
     CDS             complement(9164..9787)
                     /gene="ytaF"
                     /locus_tag="LZB08_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBM7315488.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            9975..10619
                     /locus_tag="LZB08_001985"
     CDS             9975..10619
                     /locus_tag="LZB08_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315489.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLIQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10833..11891
                     /locus_tag="LZB08_001986"
     CDS             10833..11891
                     /locus_tag="LZB08_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBM7315490.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            11995..13179
                     /locus_tag="LZB08_001987"
     CDS             11995..13179
                     /locus_tag="LZB08_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBM7315491.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVINKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13386..14099
                     /gene="rpsB"
                     /locus_tag="LZB08_001988"
     CDS             13386..14099
                     /gene="rpsB"
                     /locus_tag="LZB08_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBM7315492.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14178..15089
                     /locus_tag="LZB08_001989"
     CDS             14178..15089
                     /locus_tag="LZB08_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBM7315493.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15174..15878
                     /locus_tag="LZB08_001990"
     CDS             15174..15878
                     /locus_tag="LZB08_001990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBM7315494.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15904..16461
                     /gene="frr"
                     /locus_tag="LZB08_001991"
     CDS             15904..16461
                     /gene="frr"
                     /locus_tag="LZB08_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBM7315495.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16464..16667
                     /locus_tag="LZB08_001992"
     CDS             16464..16667
                     /locus_tag="LZB08_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315496.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16696..17430
                     /locus_tag="LZB08_001993"
     CDS             16696..17430
                     /locus_tag="LZB08_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBM7315497.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17443..18222
                     /locus_tag="LZB08_001994"
     CDS             17443..18222
                     /locus_tag="LZB08_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBM7315498.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7573 a         1842 c         3213 g         5789 t
ORIGIN      
        1 tatgatatag ataaaagtat ttattttaat tgtctattat ctgaaaaagt ctttacttag
       61 aaatcataaa aatcaaatta attaggaaaa gaaagggtgt gtataatgtg attgagactg
      121 gatttgtata ttgtagtttc ttggttgtat tcgtaggtgt aatttttttt atggttgata
      181 aattgaaatg gaaggtattt gatgttatta atccaatgct tttaatatac ttaggttcag
      241 ctatacttgg atcatttggt ttatttgcac agaatgaaga tgttggaacg tatcaaaaca
      301 taataacagt gaacttcctt ccattaatgt tagtgttttt gttaattcaa tgtgatttaa
      361 gaaaaatatt aaagatgggg cctaaaatgt tattatcatt tttctgtgct acatttacat
      421 ttatgcttgg atttgttata gcattcataa tttttaaaaa ttcaatgggt gcagattcat
      481 ggaaaattct tggggcagca tctggttcat ggataggtgg ctcatcaaat atgattgcag
      541 cagctaatgc tctaggagtt gcagaagaag gacttagtta tgcaatactt atgggttcta
      601 taggatatac agtatggatg tctgtttgtc taatgtctgt gaaatttgga cctaaattta
      661 acaaatggac aaagtgcgat acaagtttta tagatgaggt tacatcaaaa cttgaaaaag
      721 aaaataaaga taatacggca cctacgtttg tagaacttat gattttactt tctgttgggt
      781 ttggagttgc gtcttttgca caaattgtat ccaagatact tccaacttca ggtatactta
      841 atgaaactat gtgggtagta cttgttgcat ctacagtatc aatattattg ggtatgacaa
      901 aaatatcaaa gcttaaaggg agtacactgg taggaaatgt gtttatgtat ttactccttg
      961 cagctacagg tagtaaagca aatttctttg gattgtcaga agcacctgtt tacataatgt
     1021 ttggattggt agtagtcctt gtacatgcta tttcatttat agggcttgct aaactattta
     1081 aacttgattt atttacttgt gaagtaggaa gtatagctaa tataggagga gtaactacag
     1141 ctccaatgat tgctggggtt cacaatcctg tattaatacc agtaggtgta cttatggggc
     1201 ttctaggaaa tattataggg acttattgtg ctcttatggt aactcaaata ttacatatgt
     1261 tagcttaata aaaatatggt ataaaattaa taatttaaat aagaaaagtc atttcaattt
     1321 aagcagaagc ttttactgaa atggcttttt tgtttgacaa gataatctat ttcaagaaga
     1381 tgttttgaca tatttagtgt ataatattgt aaaatttact aaaaacgaga tatatttaat
     1441 ttttatattg aatcgaacgt ctgtatgatg tataataaat atacagaacg aatgtttgca
     1501 gaacgggtgt tttttgaatt tattttaata atttatgaat atatatcaga atgcataaat
     1561 atatttaaat ataaagatta aaaagaattt aaaggagtgt tgataactta tgataattcc
     1621 atcaagaaga atacaagata taacattaac aaatttaaaa aatggtgaag taactttaat
     1681 tgaattggat gaaatataca agaagatggg atttttattt gttgttagtg aaggcaaatt
     1741 gaaaaagata aaaaaggaga atcatcatta gaaatgaatg aaataataaa tattataata
     1801 gtttataaaa cctaacttat gtagaatata aatacttaag ttaggttttt ttgtgataaa
     1861 gtttataagt actcaatttt ataagataaa taatggtctt aagtgaaaag taaataagtt
     1921 taaattttag taagtattat attgcataag aggtgatatt attatgaaaa tagtaaccta
     1981 caatattcat aaaggtatgg attcaaataa tagattaact ctaactaaaa tggggttata
     2041 tttaaaacaa ttggattgtg atgtaatttg tttgcaagaa gtattatatc ctcaattttt
     2101 agctttaaaa gctgttttaa atatggatgg tgtctttgcg acaaatgtaa aaaaagtaaa
     2161 tatgatatat ggtatttgta catttacaac atctaaaatg ttaaacagta atcatttttt
     2221 cttaactagc aaaaaagaac aacggggagc tctttgtata actatagatg catatggaag
     2281 aattataaat gtaataaata ctcacttggg acttgatagg caggagagag caaaacaatt
     2341 agacgaaata atagactata ggaacagatt agtggggata gttattttat gtggtgattt
     2401 taatgagaaa aatgtctttt taagtatgtt taatgatatg gctatttcac taaacaagtc
     2461 ttacttacca acatttgaaa aatcaaactc tagaatagat tacatttttg ttaataaaaa
     2521 tactgaacta aaaggataca cagtagaaaa aatttacctg tcagaccatt atcctgtaat
     2581 aggttacatt taaatttcaa tataaatgaa attagttgtt ataaaataaa caattttttg
     2641 ttaagaaata gtaaaagaaa tataataaaa aatatcaaaa ataaaactaa ttataagaca
     2701 taaatttaca ataaagttac atttgattaa attccttgtt taacaaaagc tattcaaata
     2761 taatctaagg tagttttgaa atgggaactc tattatgcaa atttaagcta gtgttaaaat
     2821 gaggtgaagt tatgggcaga agaacaacaa gagtttcaag attaggtaaa gaaaaaaaac
     2881 gaagtgtttt aattgtattg gcagctttag ttctaataat gggtggattt acttattttt
     2941 ttaacagtaa atttctgtac aatgggaaaa tcgctaaaaa tgtatatata gaaggagtaa
     3001 atgtatctga tatgactaaa gcagaggcat taaaagctat aacagataag tatactccag
     3061 aagatttaaa tcttgcttat gatgggaaaa aatatacaat atcaccaaaa gatattgatt
     3121 taaaatatga tacagaagat gttgtaaaag atgcttatga aagtacaaaa aaagggtcct
     3181 attttcaaaa cttaaaaaaa tatattgata taagagtgaa taaggcaaat atgaagataa
     3241 aagctgaata tgatgaggca aaacttagtt ccaaagtgtc ttctatagca gatagtataa
     3301 atgttaagat gaaaaatgct tctattagtg ttggaagtgg tggactaagt tatacagact
     3361 ctgttgtagg tagagaattt gatttggcag caaataaaga gtctatatat aatatgataa
     3421 aaaataaaga gcataaaact ttagaactta aagtaaatct tcaaaaacca gatataacaa
     3481 ctgaacaagt aaaaactgta aattctgtta taggtcaata ttcaactact tattcacaag
     3541 ctgtagaggg taggtcatat aatgtaggat tatcagcaag aaaaactagt gatgtattat
     3601 taatgccagg tgaagaattt tcatacaata aacttacagg acctagtaat aaagcaaatg
     3661 gttacaaaga tgcacctgtt atagtttatg gtaagctaga acaatcagca ggaggaggtg
     3721 tttgtcaaac atcttcaaca gtttataatg cggctcttct ttcgggtatg gagataactc
     3781 aagttacaaa tcattcatct gcatctactt atgtgcctaa agggagagat gctactgtga
     3841 gcgatggagg attaaactta aagtttaaaa atccatataa gcatcctgta tatattaaaa
     3901 attatgcagg tggaggaagt gtatcatcag taatctatgg taattctggt gacaaaccaa
     3961 atatatctat agaggtaaaa caaactggtc agaataaata ttctacatat cgaatattta
     4021 aagattcaag tggaaaggtc ataaaaaaag aacatatatc aaatagttct tataaagaat
     4081 taaaaaaata gataattttt tagagaaata tttgtaatat aaaagggagt gtctcaaaat
     4141 gaacttttta gtttagagac actctttttc tgtgaaatca aatatatttt catcatttca
     4201 acaatacaaa agaagcgtat ctctattttg agacagcctt ttttgtaaaa attaacacta
     4261 ttagttcttg ttttaatatt acaatcatta atatatagaa aatattccta tatattggta
     4321 taattgtaaa tagatttagt tatataaaat gtatgaggag gtgacatggt tgaattttat
     4381 aggcaataga tatgaggtag taaattctga tgacgtttta gaaattaaca aaatctataa
     4441 agctagagat gttttttaca ggaaaaatgt gttgataaaa gttataaagc acaataatta
     4501 tatctgtgaa gattttgttt caaatttaat tgatgaaagt actgctttag atgagataaa
     4561 ctctccttat atactaaaaa taatagatgt tgggattcac tgtacagaag aaacaacatt
     4621 atattatgtt gtaagtgaag actttaatgg tatcggacta gatgaattaa ttctaggtaa
     4681 ttatcttcat ttagaagcaa ttgtaaacat aatgatacaa gttttaaaag ctttagaaat
     4741 gatacatagg aatcattcat atcatggtag tttaaaatcg agtagtataa tagtagatac
     4801 agaatacaat ataaaaattt gtgattttgg aattacaaaa gcaaataatg gggttaatac
     4861 aagaagctat ggaaacagga gatatttatg cccacatcaa ctatgtataa attatactga
     4921 taaagaaagt gatttttttg caacaggtct tattctattt gaatctatat ttaaaaaatt
     4981 gccttttgga gaatcaaatt cagaagaaaa aatgctacaa tctattgata agggattaga
     5041 ttggaatagt ataaaagcaa ttaatggaaa tactgaactt ataaatgtta taaaaaggct
     5101 tcttggaaga aataataagt ataccagagc caatgatatt attattgatt taagtaaagt
     5161 aatgtatgaa aaagcatata tagaagttga agatgagaaa gaagaagagc agaaacaaga
     5221 caaagttata caagttaaaa aagaatataa aagtaaaatg agaaaaaaaa tacataaaaa
     5281 acttgttgtt gccggtctag ctatagtcat gatttcaatg attattatta gttccataat
     5341 ataaaaattg tatattaagt attttaaaat caatgtctat aaaggatata catagaaatt
     5401 ttagtgtagt cttaaattat gaaaggaagt ggttattatg ttaacaaaaa ataatgaggt
     5461 attaaacgtc taggagtatt ttaacaaata gccacctgct tttacagttt agaataaaaa
     5521 agctgtggct aaaggtcaca gctttttgct actttttaaa ggcaggagaa tattaaaaag
     5581 gagcaaatta tgagcaatat aaaaataata gcaagtaata aatgttggat tgaagatata
     5641 gcaaggaaac aacttgaaga tatttcaaag ttaaatggaa ttttaagagt agtaggatta
     5701 cctgacttac atgcaggtaa aatacctgta ggtttagcag ttgagactaa aaatattatt
     5761 tatccacata taattggaaa tgatatagga tgcggaatga ctttatttaa aactggggtt
     5821 ctaaaaaaga aatttaaaaa agacaaatgg attaagtcac ttagtaagat taaagatttg
     5881 tccgatatag aaataaaaaa cacatataaa gaagaatgtc caattttaaa tttaggaact
     5941 atagggagtg gaaatcattt tgtagaaatt caatgtataa gtgaaattta taataaagaa
     6001 caatttgaac aattaaaatt ttcatctgat gatattatga tgttagttca ctgtgggtca
     6061 agaaattatg gagaagatat tttaaaaaaa ttttatgata aagatggact tgaagttgaa
     6121 agtgagaaag ctatagatta tattaaaaaa catgataatg ctttaatgtg ggcagagcgt
     6181 aatcgtgaag ctatcagtaa aaaaattatg cagtcaatag gtacatctgg agatgttgaa
     6241 acaatattct ctataaatca taattttata gagaaaaggg aagataaatt tatacataga
     6301 aaaggtgctg tttcaagtga aagaggagct gtgattatac caggttcaag aggcagtcta
     6361 tcatatatag ttatgcctac tgaaaataca gagatatctt tatattctct atcacatgga
     6421 gcaggtagaa aatggtctcg ttcagtttgt aaatctcgcc ttaaaagtaa gtatagtaaa
     6481 gatactataa aacaaactaa atttaaaagt cagattatat gtaatgatgt aaatttatta
     6541 tttcaagaag ctccagaggc atataagaat atagagcaga ttattgaaag tttgatagaa
     6601 tatgaactta ttcaagtagt ggcaactatg aagccattga ttacttataa aggataattt
     6661 ttaaagttaa aaaagattaa aattatatta taaatagcta attttatgga acatttaaaa
     6721 gtgttttatt attagctatt tttttatttt gtagagacga taatttatag agctaaattc
     6781 tttgatatat aatgattaca aagttagact gtattggaaa ctgcacatac atgaattgat
     6841 gacaatataa gtgattccaa aatataaatg aaagtataag cttattttca ctataaaaat
     6901 tttcactgta caaaaacatt aatgacatta tttttattga cataaatata taatttatgg
     6961 aataatatta gaagcactga tataagtgtt taaataagaa tagaaaagga gaagtgtttt
     7021 atgaaaaaaa atactaacat taatgagtcc ggagttaaac ttcttaataa ataaggagtt
     7081 tgatatataa taactcctta tttattaagt tgttcgcaaa ataactatta tcaaagtaaa
     7141 tgatagggtg atttaaaaga cgatgactta taaataaagg agaaaaaata atgaatagta
     7201 atacatttga aagattacaa ctaaatgaag ttaaagactt aataaaaata cattgtgtaa
     7261 gttcgctagg aaaaaattta atagataaat taactcctag tgggaatatt ggagtagtaa
     7321 gaaggaaatt agcagaaaat aaagaagcta gaaagataat tgaaaatagt aatcacatac
     7381 cacttgaagg attgtttaat gcaggttcta caatagataa aattgaaaaa ggaatgataa
     7441 tagaacctgt tgaacttgta aatattgaag attttttaag aggttgtagg aagatgaaag
     7501 cttttatgtt agaaaaagaa ttttattcac ctacattaag ttcttatgcc ttaaatataa
     7561 cagaatgtaa aagtatagaa gatgagataa attactgtat aaaatctaat aaagttgatt
     7621 ctaatgctag taaagaatta aaaaagataa gacgaaatat agaaattaca gaaggaaaga
     7681 taaaagatag attaaataaa tttataacat caactgtaaa taaaaagtat atacaagagt
     7741 ttataataag taaaagaaat gatagatatg taataccaat taaatcttct tataaaaatg
     7801 aagttaatgg tactatatta gatacatcat caaaaggaaa tactgtattt attgaaccta
     7861 ttagtgtttc aaatttaagt acagagttaa ctatgttaaa agctgatgaa actattgaag
     7921 aatacaaaat attatcttat ttaacagaac ttatatttga gaagataagt cagataaaat
     7981 taaatataga gatactttca gaatacgata tggtatttgc aaaagctaag tatagtcaaa
     8041 aaataaaagg aattactcca aaaataaata ataatggcta tataaaaata attaaaggta
     8101 aacatccact tctaacagga gatgcagtac ctttagattt tgaaataggc aaaaattata
     8161 gaagtcttat aataacaggt cctaatgcag gaggaaaaac tgtaaccctt aagactgttg
     8221 gtttactaac tttaatggtt caatgtggat ttgatatttc tgcaaaagaa ggtagtgaat
     8281 ttagtgtatt tgaaaaagtt tttgtagata taggggataa tcaaagtatt gaaaatgcct
     8341 taagtacttt ttcatcacat ataaaaaata ttgcagagat tatgagttta tcaaataatt
     8401 ctactcttgt tctgtttgat gaaataggaa gtggaactga acctaatgaa ggagctggtt
     8461 tagcaatatc attattagaa gagttttaca aaatgggatg tataactata gcatcaactc
     8521 attatggaga gataaaaaag tttgctacat tgcatccaga atttgaaaat gctggtatga
     8581 tgtttgataa agaaacgtta gaaccattgt ataagttaac tattggaaaa tctgaagata
     8641 gtaatgctct ttttatttct aaaaaaatgg gaataaaaaa tagagtttta gatagagcta
     8701 aagaatatgt aattgataga aactacaatt tagatttagt taaaagaagt aagctacaat
     8761 ctaatattga agataaaaat agttctgaag tcattttaaa ctatacagat tataatgtag
     8821 gggataaggt aaaattacta gatgaagatg attttggaat tgtatataag cctatggaca
     8881 agtttaataa tgtagaagta ttgtttaatg ataattttat ttcagtaaat ataaaaagat
     8941 tacaacttag cataaaagca tcagaattgt atccagaagg gtatgattta gatgtattat
     9001 ttactagttt taaagaaaga aaattagaaa aagatattca aagaggttca aaaaaagctc
     9061 ttaaaaagat tcagaaagaa attagagaga atagaaaata aatataaaaa gcacaaaaga
     9121 aagcaatcta aagagaaaag tttatcttta gattgctttc ttattaacat atcttcataa
     9181 atccaatgac taacaagatt aaaccactta accaagataa gtttaaattt acgtttttaa
     9241 caaacatatt tcctagtaaa caaccaagta atactgcaat tatatttgat attaaagaaa
     9301 atagaataac ttgtatataa ttcacaccta caaggccact accaaaaccg atagctaatc
     9361 catctaaact taaagcaagt gcaagtgtaa atgcttcagc agatttaagc actttagatt
     9421 tacttgtatc taagacatca ggttcgatac ttacattaaa atttaattta aggtctaaca
     9481 tattaaaatt ataattcttt gagtgtatag attttttctt aaaataagat ttaaaaaaac
     9541 tttcaattag tctagcactt cctaataaaa ataaaataaa gaaacatatt aacattgtta
     9601 tattttgagg taaaaaatct ctaactattg tacctgcaaa aagcgatatt gctaaaatag
     9661 ctgaacaaac tacgctgata attatatttg aatataaggg aactttaact ttatttacgc
     9721 catatccaaa gcttgtcaca aaggcatcta tacaaagaga agtaactaat aaaattgatt
     9781 caatcacagt agattaacat cctttctctt aaattttgta cttatattag ttatagtatt
     9841 gtaaaaatgg ataaattgtt acagataatc caataaattg ttgataaaat ttcaacatta
     9901 tacaacacct aaataacaaa agaatcatat catataataa tataaaacca atcataataa
     9961 agcaggtgaa aataatggaa caacatagta aggttgggaa tttcggtgat ttgttgaaag
    10021 ttttattaga tgaaaaagaa ttatcaatgg gagaactaag taaaaaaagt ggaattgata
    10081 aatctactat atctcgtata gctaataata aacaaaagcc aaatataaat catttagaaa
    10141 agatggcaat acatttaaat attaacctag aagaactgtt gaaagcttca ggatatgaat
    10201 ttagaaacag caataatcaa atatttaatg cagatagtga ttttagtaac tttgatgata
    10261 ttcttggatt tgcaaattta atcaatgata agaactttaa tggaaatatt gaaaaagagt
    10321 taagtaagtg taagttatat gtccaaacag atgaaggtaa aaaattactt tttgataatt
    10381 ttaataaaaa aatagattct attgaaaaac aaggtcaatt tacagataga ttaatacaaa
    10441 tgtatgctga tttttgtaca gatggactta caataaagaa atatttattg attggaagta
    10501 tgctacttta ctttgttata tcaacagatg tgattccaga cttcgtgttc ccaataggat
    10561 ttatggatga tttagtagca ttaaatatag ttacaaaatt acttaaaaat gataagtaag
    10621 ttttagaatc atatttaata tataaataaa tcaaagtata taaaatttat tattttttat
    10681 atgaaacaaa ttaaaagcac aatgaagtag attaaaaatt taaaactaag aatctaatat
    10741 atattttgta actatttaag tatataaaat aaaataatat ccaaactaat acaaaatagt
    10801 aataattgtt ttgtactgga ggatatatat ttatgaattg taaatggata tctaaaatag
    10861 atgagagaaa aaaatgtcat agagaagcag attcttctgg atattgtatc tttcataaag
    10921 aaaataagtc tgatgaagag atacagttga tgatggatac tcttcataaa gaagagataa
    10981 gtgaatttaa tggatttgtt tttgaaaatg aatttaatgc ggaagaaata ttaacttaca
    11041 attataaaat actggatttt tctgaatcca tatttaaaca aaaagcaaat tttaaaaaat
    11101 atatttttaa aaagaatatt atttttaact atacagaatt tagagataaa gttttattta
    11161 atggttgtgt attcttagaa aactgtgatt ttaatagaac catatttagt aaacactata
    11221 taaatgaccg tatatttgaa aaagtgaaat ttaaaggacc tgatttggta gttaataaag
    11281 tagaaaattt tccacgaatg gatggaataa tttttagcat gtgtacaaaa tttgtgttaa
    11341 agaatgttga atatggaaaa agtgagtatg agcatggcaa gataaattat agaatagcta
    11401 gaaatcaagc aacaaaaatt ggagaatatg agatgatagg attttactat tataaggaaa
    11461 gaatttatag tagtaaaata atgaaacgtt ctaattatcc tactttcagt gattatttag
    11521 ttgaaaaatt ttttgaccaa atagcacgtt acacaacagg ttatggagaa aagccatgga
    11581 atatattatt agttattata gcaataataa gtgtttttgc tttattatac ttatttgtag
    11641 gcatagaaag ttcaaatagc acacttgtag ctttagatat aaataatata ggtgattact
    11701 cactaagtga gattttcaaa atgtatatgg atttatggta ttttagcatg gctacattta
    11761 gtacagtagg atatggagat atggttgcta caagtttgat tgggaaagca ttggcaggta
    11821 tagaagtatt ttttggagtt acaataggag caatttgggc atctgtgatt attaaaagaa
    11881 tgataaggta atatttgtgt aaatatggat aggtattaat tactagatat aaattggact
    11941 aaatagggaa aatttaaata taaatatact taataatcca agaaggtgaa aaatttgaaa
    12001 aatgcactaa aaaaaattat atcattagcc atgattctag tttttataat accaaattat
    12061 tcatatgcag atgataaatc tgtagacaag tattcaaaaa catctatact tatagaccaa
    12121 gaaacaggta gagttttata tagtaaagaa cctgatatga aggtaccatt agctagttta
    12181 agtaagatga tgactttttt acttgcaata gaagctattg aaaataaaga agtaaaagaa
    12241 actgataaag taaaaataga caaatctatt gcatcagtaa aagggtcaag ttataaactt
    12301 aaagatggtg aagaagtgcc acttatagag cttatgagag gtcttatgat tgtatcagga
    12361 aatgatgcgg caattgctat agcaaaacat atttgtaaaa cgaaagaagc atttgtagat
    12421 agaatgaata aaaaagcaaa agaaatagga atgagtaata cacattttct aaatccaaat
    12481 ggtcttccaa tatacgattt aagcaacccc aaaaaaccag caaaagaaaa tatctctact
    12541 gcaaaagata tagctattct tggaaaatac atgtttgacc attatgaaaa acaagtaaca
    12601 gctataactg atatggagac atatacaaat tcagatagaa gttttgaaaa aagcaataca
    12661 aatgcattat taagaattat accagaagta gatggtataa aaactggata tactggcaat
    12721 gctggatatt gcttatcatt ttctatggtt ataaataaaa atgataaaaa tgagaaaaaa
    12781 cgtagagtaa taggggttgt attaggtact aaccataaaa ataaaagaac ctctgcttca
    12841 ttagctctac tgaaatatgg aaaagaaaat ttcaatatga aaaaagtaat tgataaagat
    12901 agctttattg gtaagaaata tataaaagga atgaaagagt tagaggttac attaaaagca
    12961 aaagatgaac tttatacaat attaaaagat gatgaaagta taaagtcaga agttaagatt
    13021 aaaaatatag agtaccctgt taaaaaaggt gatactatgg gtattataaa atattatact
    13081 aattcaggtg acttacttgg aagtgtagat atagtaagta ataatagtat agataacgta
    13141 tcattaaaaa caaaattaaa aatgaaattt gcaaattaaa ttcaaaaaat ggtataaaat
    13201 acacaaaaaa atataaaaat agaaaatatg gaaaaaatat tgttatttct tcacctctat
    13261 gatatactac tagaggtgaa aaaataaaat aaatcacaca cagcattggg atccttaaaa
    13321 ggtgcctatt acatggtttt ttagggagaa gatcaagctg gaggtataaa accaggaggt
    13381 aagatatgtc agtaatatca atgaaacaat tattagaagc aggtgttcac tttggtcacc
    13441 aaacaagaag atggaaccct aaaatggcta agtatatatt cacagagaga aatggaatat
    13501 atataatcga cttacaaaaa acagttaaaa aagttgaaga agcttacaag ttcactaaag
    13561 aagtagctga aacaggaaag ccaatcttat tcgtaggaac taaaaaacaa gctcaagatg
    13621 cgataaaaga tgaagctgaa agatgtggaa tgtactttgt taatgaaaga tggttaggtg
    13681 gaatgttaac aaaccacaaa actataaaaa caagaataaa taaattaaga gaattagaaa
    13741 aaatggaaga agaaggcgta tttaatgttc ttcctaaaaa agaagttata aaattaagag
    13801 ctgaaaaaga aaaactagaa aaatacttag gtggaataaa agacatgcct gaattaccag
    13861 gtgcaatgtt cgtagttgac ccaagaaaag aaaacatagc tattcaagaa gctcatagat
    13921 taggtatacc agtagttggt atagttgata ctaactgtga cccagaacaa ttagactttg
    13981 ctataccagg aaatgatgat gctataagag ccgtaaaatt aataactggt gctatggcaa
    14041 cagctgtaat tgaaggtaga caaggtgctg aggaagaagt agctgaagac caagaataat
    14101 aatttgacaa ttaaatggta agggtcgccc ttaccattta taatatggaa tagaataagt
    14161 aggaggaaac ttaaaaaatg gctaatataa ctgctcaaat ggtaaaagag ttaagagaaa
    14221 gtacaggcgc aggaatgatg gactgcaaga aagctttaca agaagctgaa ggaaacatgg
    14281 aaaaagcagt agatttatta agagaaaaag gattatcaaa agctgctaaa aaagctggta
    14341 gagttgctgc tgaaggtcta gttgcaatag aaatgaatga tgataataca gttgcttcta
    14401 tggtagaagt taactcagag acagatttcg ttgctaaaaa cgaagacttc aaagtgttcg
    14461 ttaaagatgc tgcttgtatg gcattagcta ctgataaaga agatatagct tctttattag
    14521 gtgaaactca taaagaggga ataactttac aagaagtttt aaataataga gttgcaaaaa
    14581 taggtgagaa attagacttt agaagatttg ctaaagttgt aactaatgga caagttgctg
    14641 gatacattca cggtggtgga aaaataggtg ttcttgttga aatggaaaca gaagctagag
    14701 atgctaaagt tttagaatta ggtaaagatg tagctatgca agttgctgct atgaatccta
    14761 agtatgtttc aagagatgaa gtagatgctg aatacatagc acatgaaaca gaagtattaa
    14821 ctcaacaagc tttaaatgaa ggaaaaccag ctaatatagt tgaaaaaatg gttaaaggaa
    14881 gattagaaaa agaattaaaa gaggtttgtt tattagagca aactttcgtt aaaaatccag
    14941 atataactgt taaacaatta gttgctgatg ttgctaaggc agtaggttct gatataaaag
    15001 ttgttaaagt tgtgagattc gaagttggtg aaggtataca aaagagagaa gaaaactttg
    15061 cagaagaagt tgctaagcaa cttaagtaat aaccaaagag agaacacgca ggtgttctct
    15121 tttttaaaaa atagaaaaaa ttggatatga aggattgaaa taggaggttt ctgatggata
    15181 aaccaatgta caaaagggta ttgttaaaat taagtggaga agcattagca ggagaaagag
    15241 gttttggtat aaataatgat gtagttaatg atattgctat tgctataaaa aaaatacaag
    15301 aaataggtgt tgaagtagct gtagtagtag gtggaggaaa cttctggaga ggcagaacca
    15361 gtgaaggaat ggacagaact actgctgact atataggaat gttagctaca gttatgaatg
    15421 caatggcgct acaggatgca ctagaaaata tagatgttgc tacaagagtt caaactgcta
    15481 tagacatgag acaaattgca gaaccatata taagaagaag agcagttaga cacttagaaa
    15541 aggaaagagt tgttatattt ggtgcaggaa caggaaaccc ttactttaca acagatacaa
    15601 ctgctgcttt acgtgctgct gagatggaag cagaagttat attgttagct aaaaatgttg
    15661 atgcagtata tgacaaggat ccaaaagtac atgcagatgc taagaaattt acagaattaa
    15721 gttacatgga agttatacag aaagaattaa aagtaatgga ttctacagct acatctttat
    15781 gtatggataa taaaattcca ataaaggtat ttgagcttac aacagagaat ataataagag
    15841 cagtaaaagg cgaaaatata ggaactactg taaaataatg aagaatgaag gaggagtaaa
    15901 attatgaaac tggaaataca taagcaatta gaagagaaaa tgaatgggac aattgatgct
    15961 ttaaagtttg aatttggaac tataagagca ggaagagcta atgcacaaat gctagacaaa
    16021 ataagagttg attattatgg aactccaact ccaataaatc aaataggtgc tatatctgta
    16081 ccagaaccaa gaatattaat gatatctcct tgggataaat ctgctatgca tgaaatagaa
    16141 aaagcaatag ctaactcaga cttaggatta aatccatcaa atgatggtga ggttataaga
    16201 ctttctgtac ctgctttaac agaagaaaga agaaaagaat tagctaaaaa agctagtaaa
    16261 gctgcagaag aatttaaagt tagaataaga aatgaaagaa gagacgccaa tgagaagatt
    16321 aaaaagatgg aaaaaggcgg agaattaact gaagatgaat taaaaaaagc acaagatgaa
    16381 gttcaaaaaa tgactgataa atttataaaa gaaatagata cattattatc taaaaaagaa
    16441 aaggatataa tggaggtata atattgaaaa aatattataa ccttttaggt cttcatgtag
    16501 atgaggtaaa acaatatttt gatgagcaaa atataaatta taccgtaaaa tctattgaag
    16561 gtaaaaagga taaagaaaaa cttgtagttc ctagagttat aaaaatatct gagataggtg
    16621 atagtgtaga actgataatc acatactttt ctgactcctt agtttaagga gtcagaacaa
    16681 tattggaggc aaaatatgaa taacaacatt atatatgata tagacttaaa taacatacct
    16741 actcatattg ctattataat ggatggaaat ggaagatggg caaaagctag atttcttcca
    16801 agaactgcag gacataaggc tggagtagaa actataagag atatagtaaa agaatgctca
    16861 aaattaggtg ttaagcactt aacattatat gcattttcaa cagaaaactg gaaaagaccg
    16921 aaacttgaag ttgacacact tatgaactta ttatctacat acttaagaaa tgaaatagct
    16981 gaacttcacc aaaacaatgt aaaagtaact gctataggag atataagtgc tttaccaaaa
    17041 acttgtataa gagaattaaa ctcagctaag gaactgacaa aaaacaatac aggagttaac
    17101 ttaaatttag cattaaacta cggaagtaga gcagatataa aaaatgctct tatagatata
    17161 gtaaaaaatt gtgaaagtgg taaaatagat ataaacaata ttgatgaaga tattataaaa
    17221 aactatttaa gcacaaagtc tataccagat ccagacttgg taataagaac aagtggtgaa
    17281 caaagattga gtaacttttt actttgggaa gttgcttatt ctgagtttta ctttactgat
    17341 atacattggc ctgattttaa aaaagaagaa ctacagaagg ctatatacgt atatcaaaga
    17401 agggatagaa gatttggagg acttaagtaa ggagattatt ttatgcttac aagaattatt
    17461 gcttcactag ctctggttcc attatttcta tttgttgtat atggaggtat tcctctatat
    17521 atagctgaga cagcaatagt ttatattgca ttgcatgagt tttataaggc atttaaaata
    17581 aaagatgtac atccaatatt cattatagga tatttatttt ctatttattt agctgtaaaa
    17641 aatattttta atttaccatt agaatatact tatgcagtaa tttttatttt atttttagca
    17701 agtataattt atatgttgat gggcaaaaac aatgttatag atgtttcgat tacgttttta
    17761 ggagtttttt atataggagt atttttagat tttataatta taactataaa tggatttgaa
    17821 aaaggtagta tatatgtatg gcttatattt gtgatatctt ttatgacaga tatatttgca
    17881 tattttagtg gttatttact tggaaaacat aagctaattc ctaaagttag tcctaaaaag
    17941 actatagaag gagctattgg aggaataata ggaagtacac tttgttgtat tttatttgga
    18001 tatttatttg gcattgattt gcttcaatta gcgataatag gaagtatagg aagtgtaata
    18061 gctcaattag gagatttatt tgcatcttct ataaagagat atgtagggat aaaggattat
    18121 ggaaagataa tacctggtca tgggggtata ttagatagat ttgatagtgt tatattagtt
    18181 gcaccttttg tatatagtgc gattaaattt tttattagat aaagattggt tggatttaaa
    18241 agagcaatct ataattgcta gtaatgaaaa tagctatgta acttttaaat gcagagatat
    18301 tctgcagtta ttagttacat ggctattttc attgttcaaa aatttagaag accttgggga
    18361 attgtataag aataggtatt actttataat agtatactat atttgtagtg agtatat
//
LOCUS       DAFKMX010000076        18226 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFKMX010000076 DAFKMX010000000
VERSION     DAFKMX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.076"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            104..808
                     /locus_tag="LZB08_001995"
     CDS             104..808
                     /locus_tag="LZB08_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBM7315499.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            805..1575
                     /locus_tag="LZB08_001996"
     CDS             805..1575
                     /locus_tag="LZB08_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBM7315500.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            1572..2348
                     /locus_tag="LZB08_001997"
     CDS             1572..2348
                     /locus_tag="LZB08_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBM7315501.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            2403..3095
                     /locus_tag="LZB08_001998"
     CDS             2403..3095
                     /locus_tag="LZB08_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315502.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            3114..4538
                     /locus_tag="LZB08_001999"
     CDS             3114..4538
                     /locus_tag="LZB08_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7315503.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(4778..7246)
                     /locus_tag="LZB08_002000"
     CDS             complement(4778..7246)
                     /locus_tag="LZB08_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315504.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(7249..7917)
                     /locus_tag="LZB08_002001"
     CDS             complement(7249..7917)
                     /locus_tag="LZB08_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315505.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            complement(8203..8730)
                     /locus_tag="LZB08_002002"
     CDS             complement(8203..8730)
                     /locus_tag="LZB08_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBM7315506.1"
                     /translation="MKSFANNITFSHIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIIAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            complement(8947..10107)
                     /locus_tag="LZB08_002003"
     CDS             complement(8947..10107)
                     /locus_tag="LZB08_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7315507.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKAPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYAKNTVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            complement(10095..10769)
                     /locus_tag="LZB08_002004"
     CDS             complement(10095..10769)
                     /locus_tag="LZB08_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315508.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            complement(11591..12418)
                     /locus_tag="LZB08_002005"
     CDS             complement(11591..12418)
                     /locus_tag="LZB08_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBM7315509.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            12800..13120
                     /gene="sugE"
                     /locus_tag="LZB08_002006"
     CDS             12800..13120
                     /gene="sugE"
                     /locus_tag="LZB08_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBM7315510.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(13253..13933)
                     /locus_tag="LZB08_002007"
     CDS             complement(13253..13933)
                     /locus_tag="LZB08_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBM7315511.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            14329..15381
                     /locus_tag="LZB08_002008"
     CDS             14329..15381
                     /locus_tag="LZB08_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBM7315512.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            15428..15844
                     /locus_tag="LZB08_002009"
     CDS             15428..15844
                     /locus_tag="LZB08_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315513.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSIKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            15873..16346
                     /locus_tag="LZB08_002010"
     CDS             15873..16346
                     /locus_tag="LZB08_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315514.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            16393..17151
                     /locus_tag="LZB08_002011"
     CDS             16393..17151
                     /locus_tag="LZB08_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315515.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            17175..18014
                     /locus_tag="LZB08_002012"
     CDS             17175..18014
                     /locus_tag="LZB08_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBM7315516.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
BASE COUNT         6313 a         2409 c         3062 g         6442 t
ORIGIN      
        1 ttaaatttta ttaaaattag taggtgttat agaattgtta ttttttcatg ttagtattct
       61 cttagtaaca gatttaaggt attacaagaa gggagaaatt aatatgaaag atttaattct
      121 tgaaacaaaa aacttatcaa aaagatatgg agaacaaatg gcagttaaca atgtttctct
      181 acaaattgaa cgaaatagta tatatggact tttaggacca aatggagcag gaaagtccac
      241 aacacttaaa atgcttgttg gtcttcttcg tccaacaaat ggtcgtatta tttttgatag
      301 aaagccttgg aaacgagaat ctcttgcaaa aataggttct ttaatagaat cccctgcatt
      361 gtatggaaac cttacagcag aggaaaattt gttagtacat acaaaaatat tgggaatacc
      421 taaagataaa atttatgaag tgttggaaat agtaaatttg aaaaatacag gtaaaaagag
      481 agcttcacaa ttttctatgg gtatgaaaca aaggttagga atagctattg cacttttgag
      541 tgacccagag ttgttaatac tagatgaacc aaccaatggt cttgacccct ttggcattca
      601 agaattgcgt gaattgattg cttcttttcc aaaaaaaggt ataactgtaa ttctttctag
      661 ccatattcta tcagaggttg ctcaagttgt agataatata ggaattataa atggaggacg
      721 actattattt caaggaatgc ctgaccctaa tgaaaatcta gaagaatttt ttacagatgt
      781 tattcttaaa ggaggacaaa acagatgaat gctttacaat cagaactttt aaagtataaa
      841 agaacattta tgggtaaact tattgttttt ttcccagtgt cttttgcagc atatgcattt
      901 ataatgcaat caacactcat gcaaaatcca ttatctcaaa caacttcttg ggcgtggcaa
      961 aatcttttag cattaatttt taactggtgg tcatttttat ttctgcctat tggatttgca
     1021 ttgtttgcta ctttggtagc tttccaggaa aaaaaagctg gaaattatcg tgctttacgt
     1081 acacacaatg tgtctcctat gacactttgg attaataaag ttatagctat ggcagtctac
     1141 agttttattt caacgctagt tttaatagtc gtaacaatta taactggttt aatattgaaa
     1201 gctggtccag ttccttttgg acagattata ggggcaagta ttgtatgctg ggttgtttct
     1261 cttgctatac ttccacttca gctatggcta gctacatgga aaggtatgtt cctaagtatg
     1321 ggagttggag ctttaggtat gatatttggt gtccttgctg cgacaaaacc attttggatt
     1381 gcagtacctt ggagttgggc agttagaatg gtatgcccaa tcatcaacat acatcctaac
     1441 ggtactatat tagaagctgg agacccactt ttgaatactt cagtgattcc tattgggatt
     1501 gttgtttctt tggttgtgtt tatagtatta acagcactta ctgcagcttg gttcaatagg
     1561 agggatgaca aatgataaag ctattatcat cagaatggct tcgagcaaag aggacagctg
     1621 tccagtggct tactctttgt atgcctatta ttttttcatt atgtgtggtt ttttatctca
     1681 caactaaatc tggaagtaca caagaatttg cttttgaagg attttttaca gtatggactg
     1741 tattcatcat tcctattgga gtaggtattt tagcaggatt tatagtgcag gaggaagagc
     1801 ttgctggaaa ttttaatggt tttttatgtg taggtatttc tcgtgttaga ttatatctgg
     1861 gaaaatttct ttttcttctg ttttgcctaa ctatatgcac atttattgct acgttgatac
     1921 tttgcatagg aatgagtatt gcagtgccat ctggagcaac aatatggtta ttcctgtcag
     1981 cagcaggact tgtggtgatt gggacacttc cactattagc tattcatctt tgggtgagtt
     2041 ttgcatgggg tatgggtgca tctattggta ttagtatcgg tggaattttg atggcagcaa
     2101 tacttggatt aactagcgtt ggtgaaaaag tatgggcatt tgttccatgg acatggccag
     2161 taaaactagg tatgcttcca ggaatttatt ttattaaaga agctggaact atttcaactg
     2221 aggcatttta ctctaaagtg atgcaaacag ctagtataga gcttatagta gtagcaatag
     2281 gtttgattat attcttgata ggaggcgtta tttggtttaa aatgtgggag ggaaggaaaa
     2341 gttattagta gacgaatatt gttttaatac tgaccttccc ataagagaaa ggtggtgtta
     2401 tattgagtaa aatattgatt attgatgatg aaatggacct agtaatgcta cttgaggatg
     2461 aattgaaggc aaaaggacat gaagtgttga tagcttatga tggtcaggca ggtatagagt
     2521 tatcaaaaca ggagcctgac cttattatac tggatattat gatgcctaaa atgaatggat
     2581 ttgaggtatg tcaggcaatt agagatgatg ttttatgccc aattatattt ttaagtgcaa
     2641 agcaatcaga aaccgacaaa atcaaagggt tgacctttgg aggggatgat tatgtaacaa
     2701 agccatttgg actgcgtgaa cttatggcaa ggattgaagc taatctgcgt agggaaaagc
     2761 gttcacagta tatcaatgaa gagaacaaga gatctacttt gtattttggg aagttatgct
     2821 tatatatgaa ggaacgtgca attaaaattg atggtaagaa tattgattta acaaaaatag
     2881 agtatgatat tgttgaatta ctagcactcc atgcaggaca agttttctca agagaacaaa
     2941 tctatgaaaa agtatggggg tgtgattctg aaggtgattc ctcaactgtt gttgaacgag
     3001 taaaaaaaat acgagctaag ttttctgctg tgacaccaga aaaagaatat atttccacag
     3061 tttggggtat tgggtataag tggaataaaa tgtaaagaga gagaggttat taaatgaaaa
     3121 atcaatccct tattacccaa tttagacata cttttatttt tattataata gccagtatag
     3181 ttgccacagt gattacttat gtatttgctc tctatttgta tattcattcc ttaaataagg
     3241 atatctatcc accaaactat tatgagcgac aagttcctag aattgaaaaa tacatcaatg
     3301 aaaaaaacat aactttgtta tcacaatcga atgaagaagg tttaaaaaga acgattcgtg
     3361 gggatgatat gctatatcaa gttgtggata ataatggcaa tattttatat ggtactaacc
     3421 ccaaaaaact atttaaaaca aaagaagaat tatttaataa ttttatcaat aaaactgttc
     3481 gtaaaggtgg ttatatccat actgttccaa tcaaagggga taatggaaag attgagggtg
     3541 ctgttattct attctaccaa gtgaaaatca cctttgcaaa tattagaggg agatttgtat
     3601 ttgcagttat tataatggct ctgttttctc catttctata tatcgtgggt tttactagat
     3661 ggctttctaa gcgctttgta aagaatatca atcaaccatt acatctattg attgatgcgt
     3721 ccaagaagat aaaagaaaag gaccttgatt ttgaaatcga ctattattca gataatgaac
     3781 tcggtaaact ttgtagtgca ttttctgaaa tgaaagacga attaaaaggc tctttatctg
     3841 cacagtggaa gatggagcag gaacgtgttg aaatggtgga agctctagca catgatttaa
     3901 aatctcctct ttctattatt ttgggatata cagatgcact tattggtaat aatacagatg
     3961 acaatgaaaa acttcatagg tatcttacag tgataagaga aaatacagaa aaaagtgctg
     4021 ctcttgtcca aaagatgcaa tatacgtctg acttagaaaa gtcaaatata caattaaatc
     4081 ttgttcctat aaatttacct gaatttttga ggcaaaaggt gcaagactat gaattacagg
     4141 ctcatcaaaa ggaagttgaa cttatattga aaatgcaggg gaatatacaa tccccaattc
     4201 aaatcgacgt tgataggttg acacgaattt ttgataatat catatccaac agtctgcaat
     4261 acactccatc aggtggtaat attagtatta ctgtaaagga tgaaaaaaat tgtatttctt
     4321 atgagatttg tgattctggt agagggttta gctctaaaga tttaaaaaag gctcttgata
     4381 aattttatcg aggagatgaa gctaggcaga caaaaggtgg acattctggt cttgggcttt
     4441 acattgtcaa acaattagtg gaacagcttg gaggttctgt taaaatagaa aattcaaaat
     4501 caggtggagc atgtgtgaaa ttttggcata gcatttaact atatatttta aattaaagga
     4561 gagtgtctca aagcgaaatt tttaattcgt gaggcactct ttttttgtga agttaaatat
     4621 attttcatta tttcgacaat gaaaaaaggc ttatctctat tttgagacag gcctttttta
     4681 aagcttatat tatagttaaa aagatttatt agttagattt gaaagaaaat agagacagtc
     4741 attgataatt taatttatat tattatctgt aaaatagtta taaattgata tctatacagt
     4801 caattatatt catcttttta acttttctta acggaataag agtagctata atacatgcaa
     4861 gtacagaaat aatagtagct tgtaaaattg aagtaattgg tatattagtg aaatcaagct
     4921 tttctatagt tgccatgttt atgataatag cactggcata cccagcaatt gaaccaaata
     4981 tagcagcaaa aattccataa taaacacctt cccataaaaa cattttatat aagctactat
     5041 tactcatacc aatagctctt ttaactccaa tctcattagt cctagtattt atatttgtat
     5101 gagttgtgtt aatgatgttt aaagtaccaa tcaatcccac aaataatata actgcccatg
     5161 ctagaaattc tatttgttta aaactctctt taagttgttt atctgattca atatatgata
     5221 tccaatgaga gccaggatta ttttcacaaa tctgttcaat agacaatctt atttgttcta
     5281 attttgattt atctttagca taaatattta tctggttgaa cttgttttta tttgtcaagg
     5341 tgttataaac agaatcatat acaataacat caacaccatt tatccagcca ttaccttgta
     5401 aaaagaacat tttatctact gtactatata tatctagttg ttttttgttg attgtgatac
     5461 tatctttagg ttttaaattt gtatgtctag tttttacacc tggtgtagaa atagcaatcg
     5521 gattttttat aaaacagata gaaccatttt taaagtcttc tagtatagaa tctgtaatag
     5581 aaggcattaa gtcttttaaa gtttgctcat caattccaat gatttttaac atctcaccac
     5641 tattttcaaa atttatatct gtatctattt ccccctgttt gtattcagag tactttataa
     5701 aggaagtgcg attgacgttt ttcattttag ctattttatc tagtgttgat ttatcaaaac
     5761 cagatatttc atttgttaag gaaaaatctc cttcttttaa attacttact gacctagata
     5821 catctaatac attagagaac tcactcagtg caacgaatac tgttatactc ataaataatg
     5881 atagtatagt tattatagtt cttcctttat ttctttttaa gtttagccat gctagatgtc
     5941 tttcaaagtt ttttattgtt ttttctcttt taatttttct tttgattttg gtggtactac
     6001 caaccatagc aattttggga gagacatgtg aagcatatat tgctgatggt aatgctgaaa
     6061 taagtgaaaa aatcaaagat acattagtgc ttaatactaa tgggaacata tatgctgttg
     6121 tatttttatg tataagctca gtaatttctt tattatcatt tgctagaagt atatctggat
     6181 ttaagatatt agtgaccatt ccagttatgc ctttcgaaga tataattcca attacagctc
     6241 caataggtat ggcaacagta cataaaatta agatttgtaa aataactatc ttatatatct
     6301 ggtttggttc agctcctatt gctcttaaac atccatactc tttaattttc tttgtaacag
     6361 agatttttaa aatattatag attacaagtc cagaagccag taaaaccaat aaggcaacaa
     6421 ataaagatac caatataatc atagatatac catcactatt agaactattt tcatcttttt
     6481 caaactgtat cttaagagca tttaagtaaa tccaattata ttgaattgaa ttgtgagaaa
     6541 gatttatttt tttagctaat tgatttacta tttcttgaaa cttttcttgc tgttttattt
     6601 taaaatccaa agaggataaa atatatctct taggtaatag attttctgat gtacctttac
     6661 ctacaatacc atttactata ccacttacat atcctgtata atcatcctct aaaatgccac
     6721 tcaatttaaa gttagctgtg taattatatg gtggtatggt atcatttaaa agtgaaatat
     6781 ctaaattcat aggtatagtc actcctagtc ttggttttat acccataagt tttagtgtgt
     6841 tttcatctaa ggctatttcg tttttatctt taggcaaatg tccagatttt aatttcatta
     6901 attttgggta gttagaaagt cctgttttat catactcttt tacaagtaca gagataccag
     6961 aatcctttat tttactactg ccaatagtta ttgaagtacc agcttgataa actctatcat
     7021 ctttctttat gtcatcaact tgatttttag tgagttggtg aaaggataca tgtctatcac
     7081 cattaaaaga acgggcttgt tcaattctta aattttgtag gataccaatt gactgtccaa
     7141 ccacagttgt cataatagtt gatagtacta ttgctataat gatgaatgta gtcataagtt
     7201 tctggctttt taattctttt attgaaagtg aaaggtaatt cttcatttct atttcacctc
     7261 cataagaaca ccatcaataa ttgaaatttt tctatcagca agttttgcaa ttctatcatc
     7321 atgagtaatt accactaaag tctttccaaa ctgttttgaa gtttttataa gaagattcat
     7381 aacttcactg ccacttttac tgtccaagtt tcctgtaggc tcatcagcaa atattatatc
     7441 tggttttgca ataagtgctc tagcaattgc aactctttgt tgctgaccgc ctgaaagttc
     7501 atggggaaga tgattgagtc tatctgcaat tccaagtaat gatgaaattt catttaaata
     7561 gtctttatca ggttgttttt tatctagaag tacaggcatt gaaatatttt cagatgctgt
     7621 aagaacaggt aaaagattaa attgttgaaa gacgaatcca aaggattttc ttctaagtgc
     7681 agatagttct ttttcagtgt gtttatatat atctttatca tcaagtaaca cttgtccaga
     7741 ggtaggatta tctaatccac ttaaaatatg taaaagtgtg ctttttccac ttccagaagg
     7801 tccaattaca gaaacaaatt cacctgagtt tatttctaaa tttacattat taagtgctat
     7861 aactttattg ttattagaac catatatttt tgataattgt tttgctttta taatcataaa
     7921 aaacactctc cttttctata taagaagagt gtaagtttta aaactcaatt ttttcccaac
     7981 ttttagctta aatctaaaaa gtcttcacta tttgcggata attcgcagtg ctgataagca
     8041 gtttcagtat catttttaat ttaaaatgta atttatgcac taaaggcttt acaatacaac
     8101 tctattgctt taaaactatt ttacacataa acttgtggat aattttttat gttttacttc
     8161 ctcattatta tataaattat ttttgtcaca aattctaagt tttcacataa atatactttt
     8221 tatatcacga tttaattctt ttgattttat cataattgct atttcttcaa aagaagaaag
     8281 aaaagccacg atacatatca taattcctgc aacatcaaga ccaaagcaga tatagagtaa
     8341 tggaaacgca aagatagaaa ttccagtgat tttattcatc aaagtatgaa gcgatgaaaa
     8401 tgtgcgatac ttaaaaaaac caatactgta actaatcaac cgtaacaatg ctattgcaat
     8461 aatacaccac catagataca tgggaagagc aatatttttt ccgacagtaa ccaaaattgt
     8521 aaccgcaaaa atgaaatcag caatactatc taactttgca cctattccac tttgttgtct
     8581 caactttcgg gcaataagac catcaagtat atcactaatt ccacaccaaa aataacatat
     8641 ccagaaaggc agtgaacaga atgtagaact aagcattagc aatgcaaaca caatatgaga
     8701 gaatgtaata ttattagcaa aacttttaat ataatcacct ccaaaattct aatttcatac
     8761 tattctacat atttaaatat atatttctat tattgtttca aatgcctttt gccaaaatat
     8821 tataattgaa gcaactctta caacttctac aaaatagtat atatattggt tgtaaattga
     8881 aatacatttt ataaattcta aactaacaaa gcttatataa gaacagtgaa agcttacaaa
     8941 atcattttaa tttcttgttg agaatttagc agtaatttta gcactgttag tagaattatt
     9001 ttcaatagaa atagagccac catgtttttc gcataatatt tttgagatat aaagacccat
     9061 accaaagtgt gcattgctgt catttaaagt cttatctcta tagaaaggtc ttaaagctaa
     9121 ttttagagat tccttactaa atcctatacc atcatcttca attacaaagg taaggagttc
     9181 ttgttcaaga tatataagta tggatacagt atttttagca tatctaaaag cattcgatat
     9241 tatattttcg acgactctaa gaatgatatt ttcatcaaaa aatagattta tattatttat
     9301 ttgattttta agtgtaaaag attttccttc ttgtttagca atgatagata tattttcatc
     9361 taagttttct ataaacttgt ttatattcga catagatttt gaaacaacta aatcttctaa
     9421 tcgttgagca ttactcatat tttctacata gtattcaatt ctgttaatat gctcagacat
     9481 aagttgtgtg gttgataaaa tcttctcatc actaagctta cctgttggta tgtattttgt
     9541 taaatagttt gaataacctt ttagaactgt taggggattt cttaaatcat gagaaaaagc
     9601 agcattaagt tgttttctat cattaatcat agtccacatc tttatgttgt ttttatttag
     9661 ttcagacctc attttttcaa aagcattaca tagattacct aattcatcat tattattata
     9721 ttctaagcaa aagtctaaat tgttgttgga tatttccatt gcacctttat tgagtatttc
     9781 tatgggtgtt tttaatttta ccttgtaaaa aattatatca gctaataata aaagtcctat
     9841 aaaaaatata agtggaagta caagctgaag tatgttgatt atattaagta atgttttatc
     9901 ataatcacta aattggttca cattgttttg aactatggtt ataatacctg tatttatagg
     9961 tgcttttaaa tacttatctt gtatagaatt atatagatta ttaagtaaaa tgtatgcgaa
    10021 agtagtaagc actgatgcta taattagtga agataaagta agaacaaaga aagctttttt
    10081 taatgacata ttatttattt tatccattta taaccaactc cccagactgt ttctatataa
    10141 ttttcaagag tatgttttga tagtttgatt cgtatttttc taatgtgttc cattataaca
    10201 tcactatttc cattgctatt gtatccccag actctatcat atattttttc tttatcaaat
    10261 acctgtccag gattcataga gagaagctct actatatcaa attccttttt tgaaagtgaa
    10321 atctgattat tatatataca aattaacctt ttttcgtaat ctattgttaa atcattataa
    10381 aatttaattt tagtttgatt tgacttcctt tgttcacgtc ttaaatgggc agatactcta
    10441 gctccaagtt cgtctaaatc aaaaggtttt acaatataat catcagcacc agcacgaaaa
    10501 ccatttatct tgtcagtatt ttcaattcta gctgtaagaa atagaatagg gcatgataca
    10561 taatctctaa ttctattaca aacttcaatt ccatctattt caggcatgtt tatatcaagt
    10621 agaatgatat ctggattttt agatattttt tttaatgcgt cttttccact ttgagcagtg
    10681 taaacctcat aaccattcat ttcaaagtaa tcttttaaga gtgttattat accttcctca
    10741 tcatctacga cgagtatttt attgttcata agaatgctcc cttctaaatt aagtttgtat
    10801 agtatgtata ttttagtata aaaatctcaa taaattatca aattcttagt tatatttcaa
    10861 aacttaatca ttaaagttag tatattttta tatattaaat aaatcaagga tgaaattatt
    10921 agaaatttta atagaatata tatttgatat ttaatactta ttggtatgca aaaatatagc
    10981 tgatgaaatc ttataattta agttgaaaat ggataaatag aaaggaagga ttttaattaa
    11041 ataaggtaga ttaattaaaa agtatataaa ttataaaaat gaattaatct acattaatta
    11101 gttgatatat caattattat tgtttttttc agttgcttaa aatatacttg atatatcaac
    11161 taatgttgtt ttttaattaa aagaaatttt aggaaagata atatcaacta ggagagataa
    11221 tatcaacatt gaagaaggta gagcaagatt tctacataat tgtgaacatt atctggcatg
    11281 tgtgcattgg tgttttaagc aagtaattca atagaaagat gtgactaaaa atagaaagaa
    11341 ataccataat ccaaatgtaa agctagaaga cataataagt aaaaaagtaa agatatttaa
    11401 taaaagatat aactaatagt gattaaatta gtaaatgctg ataattagca taatcattag
    11461 taattattag tattatttaa ttaaattaat tttagcatta tttttatgaa attaatttta
    11521 aatcaaatta atgagaaccc cataaatttt tttaacactt aatttacgat ttatggggtt
    11581 atgttctgtt ttatttcaaa gtattaagtt catataaatc agttcttctg tgcttaagaa
    11641 gtggtaactg ttgtcttatg tcaggaatta aatctctatc aatgtcagca taaataatac
    11701 attctttttc atcagcttga gcaattattc ttccccaagg gtctgaaatt cttgaatttc
    11761 caaaagctat ataagaagca ttcatatttc tagcaggtgc agcaccaaca tagaatatct
    11821 gattatctaa agcacgcatt cttatagata actcccaatg ggcgggacca gtagtcatat
    11881 taaatgcagc aggtaagata actatttcag cacctttaag agccattaaa cgtgataact
    11941 cagggaaacg aatatcatag caaatagcta taccaacttt tccatattcg gtatctatta
    12001 cagtgacatc atttccagct gtaagagtat ctgattcttt aaaactaact tttcctgtaa
    12061 cttcaatgtc aaataagtgc attttacgat gttttccaat tagagctcca tttttatcaa
    12121 atacatagca agtattatag atttttccat cttctaactc aggaatacta ccagaaacta
    12181 gataaattcc atgtttctca gctaatttac ttaatatcgt tgttgtttca cctggatatt
    12241 cttcagcaaa tttaggaaaa tatttatttt catatgggca gttaaacatt tctggcaaaa
    12301 ctacaatttt agctccattg atggcagctt ctgttaccat atctacagca tgattgatat
    12361 tttcaatttt attttctgta gtaatcatct gacatactgc aattttatag aaattcatag
    12421 aaattactcc tttcaatatt tttatgttat tataacatat taaataaaaa ttagatattc
    12481 ttagattgac ctaaatgggt atggagagaa gtgaataaga atattaaaag gtatataaaa
    12541 gttattttta ttatagatgg aacccatata ttgacttttt ttatatatgt tgatagtatt
    12601 attatagata aaatagaata attaatagag ttttataggg ttccgcaatt attaatgatt
    12661 ggtctggtcc aagataaaac ccacaacata gtttttgttg tgaacacgga aggacaaaaa
    12721 cctgggagat attttataat atttctcagg ttattttatt ggaaaaataa gagacatatt
    12781 tgaaaagagg gatgaattta tgaaatggtt atttttgttt gttgcaggtt tgtttgaagt
    12841 gttttgggct tttcaactaa aaaattcaca tggattttct aaattgactc caagtatact
    12901 tactgttgtt ggaatgattg taagttttta ttttttgtca ttagcactaa aacatcttcc
    12961 acttggaact tcatatgcaa tatggactgg aattggtacc gtcggtacag ctattgtagg
    13021 catggctatt ttaaatgagc ctattagtat atcaagaatt gcatgtatag gttttattgt
    13081 ggtgggaatt gtagggttaa agctattatc tactaattag agtatattaa tttaaataag
    13141 ctatgaaatt aaaggcgaga aaatgtttaa aaataatcaa catttaaaaa gtctttactt
    13201 catagcttat cttatttata agtttattat atttgtaaat tatatgtata tactatttat
    13261 ctaataaaag agcagttaaa tccatagggt caactattac accatcttta taaacacgca
    13321 tatttccaga tgctatttca tctattaaga ttacttcatc attgtagaat ccaaattcaa
    13381 atttaatatc ataaagctca agaccttttt cagctatagt atcacgaact atgttagaaa
    13441 ttttttgagt catgcttttc attgaatcaa attgagcttg agtcataatg tttaaagctt
    13501 ctaaaccttc agatgttata agagggtcac aacggtcatc atcttttaaa gttgcttcta
    13561 cgtaacaatc tagtttagca ccttcttcaa cacatgaacc ataacgacgt aagaaactac
    13621 ctacagctct ataacgacaa ataacttcta atccattacc aaaaactttt gctggtagaa
    13681 cttccatagt tgcttcttct aaattagcac ttacataatg agttttaatt ccagcattgt
    13741 ttagaatttc aaagaactta acagatgtct ctaaattagc tctaccaatt ccatctatac
    13801 ttagacctac actattttcg cctgggtcaa atactccatc tttaccagtt acatcgtctt
    13861 taaatttaag taagtaattt ccattatcta attcaaaaac atccttagtc tttccttgat
    13921 acactttttt cataaacaac ctccaaaaag ttaaattatt atattgaata aggcattagt
    13981 gtcaaaaaag ttctgatatt cgcatgaaaa tttatattga taccttatat ttaatattat
    14041 atcacaaata gaagggaaat ttccgaacaa ttttataaaa ataacaaaaa aagtacattt
    14101 tataaaactt ataataagag aactaatata aaatgttagt gtcgtttata ttgacacaca
    14161 taagttttaa tataagaaac ttttgacaca ctttgacaca catttaatga taattttata
    14221 aaatgcatga atacaatgct tacatatatt ggcatgaaag ttgctttaga tatttaacat
    14281 ataaacaata gggatatatt ctcaaataaa aagtaggggt ggttaatagt gaagtcagtt
    14341 agattctatg gtataagaga cacaagagtg gaggatgtgg atgtaccaaa aatattagaa
    14401 aaagatgatg taataattaa agtaaaagta gctggaatat gtggttctga tatatcaaaa
    14461 tattcaaaga caggtccaca tatggttgga gaaattttag ggcatgaatt ttctggagaa
    14521 gtggcacagg ttggtaaaga agtaaggagt tttaaaatag gagatagagt tgctgtttgt
    14581 ccagctatgc catgttttga atgtgatgaa tgtaaaaaag gcttgtattc aagatgtaat
    14641 aatgtagcaa taataggaaa taaagaactt ggaggttgtt ttgcagagta tacaaaggtg
    14701 aaagagagaa atttaataaa aataccagat gagataagtt atgagactgc cgctgcacta
    14761 gaaccagtat gtatagcagg acatggtctt tttaggtcag aagctaaagt tggagatact
    14821 gttgtagtac ttggtacagg tccaatagga ttattttcaa tacaatgggc aaaaatattt
    14881 ggttctacaa aagtaatagc tgtggatgta tttgatgaga agttagactt ggcaaaagag
    14941 ttaggggcag atatttgtat aaatgcaaaa gaaaagaata ttgtagaaga aataaaaagg
    15001 ttaactgatg gagatggtgc agatattgtt attgaatcgg ctggtactcc actgacatgt
    15061 ggtcaagtac tgttgctagc taaaaaggga ggtacagtat tgtatgcagg tgtaccatat
    15121 ggggatgtag ctttgactag agagcaattt gaaaagatag taagaagtga attaactgta
    15181 aagggaactt ggtttggaaa ctcctttcca ttcccaggaa aagaatggag tgctggattg
    15241 tatcatatgc aaaagggtga tatgaatgtt gaaaaattag taacacatag aataaattta
    15301 gaagaagtac cagcttattt tgaaaaagta tacaaaaggg atattttctt tggtaagata
    15361 atgattaata tagataatta gattagagta ctaaataata atccatataa aaaaggttaa
    15421 gaaaggtatg attcatatgg caggaataat tatagccaca catggaaata tggctaaagc
    15481 aatgttagaa agtgctgaat taatagtagg caaacaggaa aatgtagaaa ctttaggatt
    15541 aaaccatggt gacagcatag atgagtttag tataaagtta gaagatggaa ttgagaaata
    15601 taaggatgaa ggggttttag tattgcttga tttttatggc ggaactccat ttaatacatc
    15661 tgctattata ataaataaat ttgctaaggt atgtgagtta gaatgtttga caggtgtaaa
    15721 tttacctatg cttttagagt tatttttaaa cagagatact atgaagttaa aagacttgaa
    15781 gaatttatgt gaggaagttg ggatatcagg aatcaaagat gttaaaactg ttttaaagtt
    15841 gtaaaataaa ttaaaaatct ggaggaataa ttatgagtaa tatagcatta acaagaattg
    15901 atgatagatt aatacatggg caagttatta ctgcttggtg taagattaca tcggcaaaaa
    15961 ggatagtaat cgtggatgat ttagtagtaa aggacccatt tatagttcaa gttttacaaa
    16021 tggcagctcc atcaactgtt aaggttgaag tacatgatgt agaatcaggt gctgaagtct
    16081 taaaatcaca taatggagat gaaaatttaa tagtattggt caaataccct aaaactgtct
    16141 taggtttagt aaatagcggg gtagatttaa aggaattgaa tgttggaggt atgggagcag
    16201 ggcttggtag aaaaagtttt tataaaaata tatctgtgtc agatgaagaa aaagaaatat
    16261 ttagagagct aatatcaaaa gatgttaaat gctttataca aatagtacca gatgcaaaga
    16321 aaattgatgt aggaacatta ttgtaaagaa gactatcttg taaatcttta aaagaaaata
    16381 gggaggaaga atatgcacat ttcactaatt caagcagtat taatagcaat tttttattat
    16441 ctttcttgga gtccgtggtt gacttatgtt ggatttttta catggaatag acctctattg
    16501 gctggatttg taacgggaat tatattagga gaccctgttc aaggagcaat tataggagca
    16561 ggtataaata tgatatatct aggttttata tcagcagggg gagctcagat gggggaccca
    16621 gcttttgcag gatatgtagg tactgcttta gctatagcat ctaagctaga tgtaagtact
    16681 gcaatggcaa tagctgtacc attgggaact gttgcaactg tactttggat tggaaagatg
    16741 acagtaaatt ctttttttgc acattgggct gatagagagg ttcaaaaagg gaatgttgat
    16801 agagtagcat ttataaatat agtaccacca cagatactgt tatttgcaat gagttttata
    16861 ccagcattat tagttgttta ttttggtcca aatgcgatag atggtatgtt ggaagtaatg
    16921 aatgataatg tgttacatgt atttaatgta attggggcta tgttgccagc acttgggatt
    16981 gctatgaatt taaaattgat tggaaacaag tttactatgc cctttttcat attaggaata
    17041 ctaatggctg tttattttaa agtagatata atagtaatat ctgttattgg agtaatactt
    17101 gctcttacaa ttacttcaat taagtacgga aaagatagtg ctacatctta aatacatgtg
    17161 agggggaaaa tattatgtca gatataaaaa atgaagtatc taagaaaacg ttaagcaaga
    17221 aagatgtaat aaaatcatgg ttaagatggt ttttctttgc acaatctaat tacaattatg
    17281 aaagattgca atcaacagct ttttcacatt caatgttgcc agtacttaaa aaattatatc
    17341 cagataagga agagttaaaa caagaagtgg agactcatct agcatttttt aacacagaac
    17401 ctatatgtgg atgtgttata catggaataa caatagctat ggaagaagaa aaagctaatg
    17461 gagcagatga aataagtgga gatggtatga atgctatcaa aacaggcttg atgggtccac
    17521 tagcgggaat tggcgatact ttaactcaag gagttataac tcctatagtg ttggctgttt
    17581 gtattggttt aactgaggga ggagcatcaa ttgcaggacc tatattattt gtaatagctc
    17641 aatatataat aatgacttca atttcttttg gaatgtggac aaatggatat aagtatggga
    17701 aaaaagctgt tgaaagtata cttcaaggtg gaatagtaaa taaggttata gaaggggctt
    17761 ctatactagg taccttagtc atggggggac ttgtaggtag gtttattaat ttatctacac
    17821 caataagtta tactagtggt gattttaaat ttagtcttca aacggattta cttgataaga
    17881 tatttcctgg gttaattcct ttggtactta ctttattagt actctttgct ttaaaaaaag
    17941 gtctttctcc aataaaaata atgtgtatac tgatagtagt gggagctatt acaggtattt
    18001 taggactatt ttaaataaat atatattggt gtaagttagg attctatatc tattgattat
    18061 atttgtagga tgttggaaaa tttatattaa aaagtttggc aattagggta attttataat
    18121 agattaaatt ttaaacttat gtaagctata tgtagtattt aatattttaa aaagtatctc
    18181 agaaagattt ttaatcagaa tgaggtgctt tttcattttt atataa
//
LOCUS       DAFKMX010000077        18207 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFKMX010000077 DAFKMX010000000
VERSION     DAFKMX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.077"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(200..1876)
                     /gene="bclA2"
                     /locus_tag="LZB08_002013"
     CDS             complement(200..1876)
                     /gene="bclA2"
                     /locus_tag="LZB08_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBM7315517.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGSDGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2254..2793)
                     /gene="hpt"
                     /locus_tag="LZB08_002014"
     CDS             complement(2254..2793)
                     /gene="hpt"
                     /locus_tag="LZB08_002014"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBM7315518.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRHINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3121..4428)
                     /locus_tag="LZB08_002015"
     CDS             complement(3121..4428)
                     /locus_tag="LZB08_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBM7315519.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4853..6628)
                     /locus_tag="LZB08_002016"
     CDS             complement(4853..6628)
                     /locus_tag="LZB08_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315520.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6625..6762)
                     /locus_tag="LZB08_002017"
     CDS             complement(6625..6762)
                     /locus_tag="LZB08_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315521.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6840..7409)
                     /locus_tag="LZB08_002018"
     CDS             complement(6840..7409)
                     /locus_tag="LZB08_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBM7315522.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7593..8228)
                     /locus_tag="LZB08_002019"
     CDS             complement(7593..8228)
                     /locus_tag="LZB08_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBM7315523.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREISDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8896..9777)
                     /locus_tag="LZB08_002020"
     CDS             complement(8896..9777)
                     /locus_tag="LZB08_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBM7315524.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9897..10904)
                     /locus_tag="LZB08_002021"
     CDS             complement(9897..10904)
                     /locus_tag="LZB08_002021"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBM7315525.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10924..11406)
                     /locus_tag="LZB08_002022"
     CDS             complement(10924..11406)
                     /locus_tag="LZB08_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBM7315526.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11424..11891)
                     /locus_tag="LZB08_002023"
     CDS             complement(11424..11891)
                     /locus_tag="LZB08_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBM7315527.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11906..12664)
                     /gene="prdD"
                     /locus_tag="LZB08_002024"
     CDS             complement(11906..12664)
                     /gene="prdD"
                     /locus_tag="LZB08_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBM7315528.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12744..13469)
                     /gene="prdB"
                     /locus_tag="LZB08_002025"
     CDS             complement(12744..13469)
                     /gene="prdB"
                     /locus_tag="LZB08_002025"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(13017..13019),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBM7315529.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13507..13791)
                     /locus_tag="LZB08_002026"
     CDS             complement(13507..13791)
                     /locus_tag="LZB08_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315530.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13872..15752)
                     /gene="prdA"
                     /locus_tag="LZB08_002027"
     CDS             complement(13872..15752)
                     /gene="prdA"
                     /locus_tag="LZB08_002027"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBM7315531.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16181..17941)
                     /gene="prdR"
                     /locus_tag="LZB08_002028"
     CDS             complement(16181..17941)
                     /gene="prdR"
                     /locus_tag="LZB08_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBM7315532.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT         5239 a         3591 c         2141 g         7236 t
ORIGIN      
        1 tatgaaatca tattgtttat tattttatag tcttttcatc atataaacct attaaatatt
       61 atcctattta ttggtatatg aaatcatatt gtttattatt ttatagtctt ttcatcatat
      121 aaacctatta aatattattc tatttattgg tatatgaaat catattgttt attattttat
      181 aatcttttca tcatataaac tattgtattc tataaactga tacatatcca gctgcagaag
      241 tacctgtcgt gcctgaacca aatgtaaaac ttaaagtaac agctgctgct ctagcatcaa
      301 ctaaaaagct taaacttaca cttacatctg atgaagtaag tgtatttact gcttttgcaa
      361 taaagttatt ttgagttact ccattaaagt tagtagtaat tgacataaat cctgcaagtg
      421 aaagtgtggc acgtataccg aaagatacat tgtatatccc tccaactgca agatttatag
      481 tactacttgt tggaaaagtt atattagttc catttataaa tgatgaatta aatgtcacta
      541 atgtattatt cactaaactt gaagaggaca ctgtaaattg tgcattgttt gctgctaaca
      601 ctcctgttgc tcctgtcgct ccggttggac ctgttgctcc atttgctcct gttgctcctg
      661 tcgcccctgt tgctcctgtc gctccatttg ctccggttgg gcctgttggt cctatcgctc
      721 ctgctactcc atttgctccc gttgctccta ctgctccggt tgggcctgtt gctccatttg
      781 cccctgttgc tcctgtcgct cctgtcgctc ctgctactcc atttgctccg gttggaccgg
      841 ttggacctat tggacctgct actccattcg ctcccgttgc tcctactgct cccgttgggc
      901 ctgttgctcc atttgcccct gttgctcctg tcgctcctgt tggacctact gctccatctg
      961 atcccgttgg gcctgttgct cctactgctc cggttggacc tattgcccca gctactcctg
     1021 ttgctcctgt cgctcctgtt ggacctacca aaccatttgc tccggttgct ccggttgggc
     1081 ctgttgggcc taccaaacca tttgctccgg ttgctccagt tgggcctgtt gggcctgttg
     1141 gacctaccaa accatctgga cctgttgctc ctactgctcc tgttggacct acctctccat
     1201 ctgctccggt tggacctgtt gctcctattg ggcctgttgc cccagttgtt ccggttgctc
     1261 cagtcgctcc tatacttccg gttggacctg ttgctcctgt atttcctgtt ggtcctgtag
     1321 cacctgttac tccatttgct ccggttgctc ctgtatttcc tgttggacct gttattccat
     1381 ttgctccggt tgctccggtt gctcctgtta gtccggttgg acctgttgct ccagttgctc
     1441 ctgtattccc tgttggacct gttattccat ttgctccagt cgctcctgta tttcctgttg
     1501 gtcctgtaga gcctgttatt ccatttgctc cagttgctcc tttgtttcct gttggacctg
     1561 ttattccatt tgctccggtt gctcctgtat tccctgtcgg acctgttatt ccattcgctc
     1621 cagtcgctcc tgtatttcct gttggtcctg tagagcctgt tgttccattt ggtccagtcg
     1681 ctcccatatt tcctgttgga cctgttattc cattcgctcc ggttgctccc gtatttcctg
     1741 ttggacctgt tattccattt gctccggtcg ctccagttgc acctctaggc cccgtcggtc
     1801 ctgttggacc agtagcacct gttggccctg ttggacctac atcttgatat aaacttggac
     1861 ctgaaatatc actcatataa ttaatcctcc ttttttaaag ttagagtatt actctataaa
     1921 tattgagtat tactctatat aattatatga atcgacaatt tcttatgtga taatgtatct
     1981 atttgcttat atcaacatat atgctttgat tcttgaattt cattactttt ttgtaaaatc
     2041 ttttacaatt attagtactc ttttacaatt attaatacaa ttaaaaaact catttcccat
     2101 caattattta aaattttttt attatgttat aaaaaataga taattaaatt gcataaaatt
     2161 aatttaatac tactttaatt actttgtaaa taaaaaaacc tcaacacgat taataaaaat
     2221 catattaagg catccacaaa attatataat tgcttatcta tcttcatcag taacattgaa
     2281 tatataagga acatttctat aataatcacc aaaatcaaac ccatatccaa ccacaaactt
     2341 atcttctatt tcaaatccac aataatctgg cactaaatcg acttttcttc tgcttggttt
     2401 atccaaaaga acacagcatt ttaaactagc aggattttta gcttttagat gttttagcac
     2461 aaaatccata gtaagtgcag aatcagtaat atcgtctaca accaaaacat catatcctgc
     2521 tatatcagta gtcacatctg aaactacttt aacccttcca gtactttctt cattgtttcc
     2581 ataactagat gtagtcataa aattaactct aagtggtaaa tttatatgtc taactaagtc
     2641 tgcacaaaat ataaagctac cttttaatag agaaactacc ataagatttt tatctttata
     2701 atctttttct ataattgctc ctaattccct taatttatct ttaatttttt cttctgaata
     2761 caatacttcc catttttttg tttctatatc cataaaaaga cctccaattg taataatcct
     2821 tcttaatttt ataagagatt gctcactttt tcaatactct tattcaatat tagtcaatat
     2881 tattgagttt ttatgttcat tcaacaatta ttaacaatgt atcttatatt ttaagtagtt
     2941 catatatcca cactaaatta catactatat attttagaca aaaaaagaaa aaacttgcac
     3001 taacaagttt tttctattat acataaattt atacataatc atattttatt agagggtact
     3061 aattaaattt atttatgcta tttttgttat acctcataat acaaaacata ttataaagta
     3121 ctagtcaact ttattcatat cattcattac atttaagatt atctcatctg ttatagacat
     3181 acctttgtca cttactctac ctaagttttg tatactttgc tcaacacttg aaccaactat
     3241 accatttagt ggtggtacaa aacaatcttg cttagctatt atagcacttt gtatagctgc
     3301 agatgctgca gaagataatt ttaatgcaca accagctttc gcaccgtcac atatcattcc
     3361 acttaagtca gctatcatat tttctatagc tccttctatt tgcttttcag taccattcat
     3421 aagccaagag ataccagcag ttgctccagt agaagcagca acaccacatc cacatacagc
     3481 agataatctt cctgtatagt tcttaatata accagttact agatgagata tagctaaagc
     3541 ttttgctagt ctttcatcag attgtgggaa ttttttatta taagcaacga ttggaagaat
     3601 tgctgttaat ccatgatttc cacttccatt tgaactcata acaggcattt ttacccctgc
     3661 cattcttgca tctgaagctc cagcagttaa catcattgca taatttataa catcattacc
     3721 taaaagacct tcttctatag actttttaat tccatatcct acaccaatac cagttttttg
     3781 ttttaatcca tattcagcca tctcttcatt catttttact ccatctagta aaaattctat
     3841 atctttaaaa tcaattcctt ctacattttt tattaattct tgtatagtta ctgtatccat
     3901 taaactttca gctttttctg ccgcatcatt tgaagctgat acttttggtt cattgtctaa
     3961 taatacttct ccatcttttt ctaagaacgt aaaattatca tgtttagttc ttattctcac
     4021 tttagcaata tgattttttc cttttaacac aacttctata aatactttct ctcttgtatc
     4081 agcaggtgtt attgatagtg gagtattatc catataatcc tcagctattt taacttcctc
     4141 ttttgttaga gtttctaaca cacttaatcc attttcagaa tgtccaccta ctatacctag
     4201 agctgctgca atttttaatc ctaatctttc agtacctggt atacctacgc acataccatt
     4261 tttataaata ctaggactaa ctaacatacg attttcaact atttcttctc ctaataattc
     4321 ctttgccttt gcacatgcta gagctaaggc tactggttca gtacacccta ctgctggttt
     4381 aacttctgcc tttaacattt ccactaattc ttttcttata tctctcatta tatataaacc
     4441 tcccaattaa gtttagattt agtactcaaa taattaatta aataatttct tatctatatt
     4501 tatagcaaaa tacatgccat attttttata tctttttata tttttagtct tatattctat
     4561 aaccattggt atgactgctt ctatttatgt atttttttta tttacataga gtatttatat
     4621 tctttaaaat ggagaaacat atgacaattt cgcaaaaaga caaaaaaaag tctcaaaatg
     4681 agattctata tcattttgag acggctcaaa tgtattattt tttaacttat aaaaaccatt
     4741 ttttaattca taaaaaaaga ttctgcaaaa cacttatagt ataagtttaa agctttcttt
     4801 aaagttatga atttagaaaa atttaaattt tatatagctt tttattatat tactatgaaa
     4861 ttatattgta tttttcaatt tttctatata atgtagctct tgatattcca agtgctttag
     4921 ctgttaattc tttatctttt ttataatctt tgtacttatt taaagctttt attatctcaa
     4981 tcttctctag ttcatcaagt ggaattatcc tatcgctttt aacttctaac tcttctacag
     5041 aggtattctt aattttttga ggaattacat tcattgtgat tattgaagaa gaagacatat
     5101 tcacactata ttcaataata ttctgcaact ctcttatatt ccctggccac ttatattcca
     5161 tcatagcatc aacaaccctg ctatctatgt caataacatt tttatctaat ttttttgaat
     5221 actctttaac catatgcttg attataagtg ggatatcttc ttttctctct cttaaacttg
     5281 gtaattttat aggtataaca tttaatctat agtataaatc ttctctaaaa gtgccatttt
     5341 gaaccatttt ttctaaatct ttatttgtag cagcaataat tcttacatct attgaaacat
     5401 ttgatttact tccaatttta ttaagttcct tttcttgtaa tactctcaat aactttgact
     5461 gtaaatgcaa actcatatct cctatttcat ctaaaaatat acttccctta tgagctactt
     5521 caaacattcc aagctttcca ccttttttag ctccagtaaa agctccctct tcatatccaa
     5581 ataattcact ctctaataaa ttgtctggta tagcagcaca gtttacagct ataaaaggat
     5641 tatctactct attgctatgg ttgtgtatgg ctcttgcaaa tagctcctta cccgttccac
     5701 tttccccagt aataagtact gttgatgttg atttagaagc tattagggct tcctttttag
     5761 aatttcttat agctacactt tctccaatta tgttttccaa ttttattcta taatctttat
     5821 tcatcttatt gtagtttttt atagcatctc ttttatctat aaaatcaata acatatccct
     5881 ttaactcatt ttttaatact attttattga tgttgtatat tcccttcgaa tcatatccgt
     5941 atcttttata aaaaaaacta caggatttgt ctttcttaaa gttatttata gaaggtttct
     6001 ttataaaatc taattcgaca aatatgtttt tgccagttat actatccttt atattgaaaa
     6061 gctctttaaa ttttgaatta tatttatcaa tttttccatc cacatcaata gacacaacag
     6121 ctttgtccat attgtttaac agtgtctcta attttttctt ttctaattca agctcataag
     6181 ttttaatttg tgcttttaat ttattagata ttaggtctgc cattttgcct aaaaagttta
     6241 taagaccact tttattattt tctataatct gtgcttgttc ctttgtaaag gctataaggc
     6301 ctataacacc atgagagact ccatcacata taatgggaca acacacctct gcaaactcta
     6361 cacaagcccc acttttatag cattccctac atatatcact actctttggg tcatcaatta
     6421 gtattgtaat ctgctctgta agtgattttt taaaagccgt atatttatct actttctcac
     6481 ctattttgtc tatatatact cctgtacctg caatcctaaa taactcctta tttactatag
     6541 ttacatctat attgagtact gatactatag cttcagctat attttgaata tcagatttta
     6601 tatattctaa acctatcata atcatcattc cttatttttc tttaacataa gtatttgtct
     6661 tatttcttta agaatcaagt atatcttatt taaagtaaat tggattgaca tcaggattac
     6721 aaagagcata acaattatta ttaactcata atttatttcc atctaaacat ctccttataa
     6781 caaaagacta ggcttaacct agtcttttat attatatcca tatttatatt ttaactcatt
     6841 tatcttcttt ttcccacata aatatatttt aatttatttg actctttaat cataccatca
     6901 aagtttaatc ttgaaaatat attaaatact tcatgtggat agtgaatctt tgcaacagaa
     6961 ccacaaaatt tatgtttaag atagtttaac ttaactttta aattctttaa atttaaatcc
     7021 tctaatataa cctctccatt gccctttaac actctttgta tttcagatga agctttttca
     7081 caatctgtaa taacattaaa ttggtcaact attattatct tgttgaactg attatcatca
     7141 aatggtagaa actctaactt acctttaact acattgcaat tttcattata cactagagaa
     7201 taacagacat cttcataatt actattctcc agtatagtta cagaagttcc aagacctctc
     7261 aatctcttgc ctaggtttcc tatatgacca acaagcaata ttttatcacc tacattatac
     7321 tcaattgtat ccaaaaaaca ttctaattca tcagatgtat atttagcttt aaccggctgt
     7381 ttatttatct gacataatat gcttttcaca atgcttcacc tcttcatttc ctcaattata
     7441 aattatgtac agattacatt ataataaatg gcgagcaact attcaagaga tatttcatta
     7501 gtattttttt aaacaatctt tatacatttt aatatgatat tataccaaat ttattaggat
     7561 tttattcata aaaatcagtc tattttttat gtttattcat tgttattttc atccaatttt
     7621 tttattttcg atatggatac ttcatatgcc atttttttga ccacattatt ttcatctatt
     7681 tttttctcat attctctact ttgaacccta ccccaaagct gtattctatc accaaccttt
     7741 agattctttg caaatttagc atttctaccc caaactattg atggtatata atcagattta
     7801 ttataaggtc tgtttatagc aactaataaa tcgcttattt cccttcctaa aggtgtcttc
     7861 ctatacacag gttccttaca tacatatcca tctaaaaata tactattagg gtctttattt
     7921 tcctcgtcta tgctttttat ttctctgaca aacactgtaa gcactaatct atttttatta
     7981 tttaatgttt tgttatatga tcttaattgg cctacgacat tgactaaatt ttgtttatca
     8041 aggtcaatat cttggagaag tctttctgat acagtcattg gaagtatatc aaaagaatcg
     8101 cttagtctgt ttatttttat tttcatatta taaaattttt ctccaaatat ctcatggcta
     8161 aattctagct tattgtctaa ctcccctctt aagtttacta cgttattctc atctttaaca
     8221 gctatcatat tttatccctc cacaattgtt aattcttata tttctggtat cagcttatat
     8281 aataaatata tttataaggg atgtatttta ttacaaatct ttgattgctg tttaaaagtt
     8341 tttatttagt tataaatttc atttaatatt tcacttttat aattataaaa ttattatctt
     8401 tttgtaccac atatactaat taaactacct atttcagttt tatattaatt ataaaaatcc
     8461 tattatatag ttaataagtc atatataact taaatttatt cactctatat aagcaacttc
     8521 aacgaattaa atttattcaa ataattcaga tgaatattga gtcattataa acttattttt
     8581 cttaattata agttatatta gacttattac aggttatatt tttattaatc tgtattaata
     8641 tatgaacatt tattgaactt attgattata aaatttaaat atttagcttt aaattatgct
     8701 ttattcttta aaaatacaac gttttttaca ttaaatctac taaatcacta tatatattac
     8761 ataattggca tgttaattgc tttatattaa tttagtaaat aaaaaactgc cctaaaatat
     8821 taattcaata ttttaaagca gtttataatc tttattatta gctataatat ttaataaaca
     8881 ctaagtagtc tttaactaag cagtcttttt gtttttattt gcagccatta tcattgtagc
     8941 agaagtataa gctataacta ctattactaa tgcttttaat atatccattg gtagagtttt
     9001 tactatatat gcagctacga aaactcctat taaaccacat atagctatta taactgatgc
     9061 ttttttagta tatgcatctt cttttatgaa tttcatactt gcaactggca ttaataatgc
     9121 acaagaaccc ataactattg ggaatgcaac ttttgcagac attcctaaga aataaaccat
     9181 tgccatacct ggagcgtata gacctatacc agctgtcatt aaagctccta gtataaagtt
     9241 acctattaca cctattacta atttaacacc agtaagacct aattcatttc ctccacctgg
     9301 taatacgtta atccaaggat gacctaataa cattaatatt gctgttaatg ctaaagttac
     9361 acccataaca agttgtattt ttctctcgtc catcttagct attattcctg cacctatata
     9421 tgaaccaact gctgctgcca caaccataga tactaaagta acaccatcaa ctggtatgat
     9481 tgatgtaaat atgaatgctt ctgtaacaac tggaatagta tgtgctacgt ttaatgtacc
     9541 aggcattttt ttatctggta tatttaactt taaagccttt gacattgcta ttgttggagc
     9601 aaatgaccct attcctaatg tatcaaagaa gtctgttata aatcctgtta cagcaacttt
     9661 tggagtactt tgttcttcta gtttcccctc tttgtttgct ttagctacgt ctttaccaaa
     9721 cacaactgca aagattgctg cgaatactac atataatgcc tttaagattg ctaccataat
     9781 ccatcctcct atttttttat tttttcatat attctgaatc ttatagccta aaagctatag
     9841 aatagcaacc gacaagtggc tactattcta ttttaatttc ctaatttatt tattacttat
     9901 ttaagaataa atccatgttt aagtgggtct tcttcatcta ttacaaaatg attaaatcca
     9961 gttatataag cagaaccagt tattttaggt actacagcat tgaaatctgc aactttagtt
    10021 tcttcaacta tttcaccttt gaataaagtt cctaatatac tttcatatac gaatttttct
    10081 cctactttta attcaccttt agcatgtaaa gtagctaatt tagcacttgt tccagttcca
    10141 catggagatc tgtcaacttg accttgacca aatataacta catttttgta agtagcttct
    10201 ggatgagttg gctcatcata tatttcaact aagtctacag tttttatatg agctaaagtt
    10261 ggatgttgta tttctatttt ctcatttatt atatctctaa gtttcatagc tagttcagtt
    10321 aatttaccag cattttgagg ttcgattttt aaacctaatt gacttgcatg tattatagca
    10381 aagaagcttc caccaaatga tatgtcaaat ttaacagttc caacacctgg taagtcaact
    10441 tcaacacctt ctttatataa gaaagctggt acgtttaaga atgatacttc tttagctttt
    10501 ccatctacaa ctgtaacgtc tcctcttatt attcctgctg gagcttccat aactacatga
    10561 gttacaggct ctactgctgg aactacacct gtttctatag ctgctgtcat agctcctatt
    10621 gtaccatggc cacacatgtt aaggtatccg ccaccatcca tgaagattat tccaaagtca
    10681 gcatctgggc aacaaggttg agtcattaca gaaccaaaca tatcattatg tcctcttggc
    10741 tctaacatta tagcagttct taaataatct agattttcct ctaaatattc ttttttctca
    10801 ggcatagaat ttccttttat gttaggtatt ccacctacaa ctattctagt tgcttcacct
    10861 gctgtatgag agtctatagc ttgtatactt ctgctaaatt tcatatcaaa ttccccctta
    10921 ggtttatttt gcgtctttac gcatttttaa ttttaggaaa gccaatgctt ttttaacatc
    10981 aacttcgtcc atgtgattgt ctccaactac ttctgtttct atgtagcctg ttgtcttttt
    11041 ataatcaatt atggtagctt tatccaaata tggattggta attactaatc catctgctcc
    11101 cataatagta agacctactg taggtatatc tgattttaca aactctgcta tagcatttgt
    11161 aaaatcaatg tgctggtttc ctgccatatc taaagttata atcactccat caggtcgcaa
    11221 tgattcaatc catcttccaa gtctagttgc taaaaatgtt ttccattcat tgtgctctga
    11281 tgtcccaact acaaccactc ctaacaagtc tatgtcagaa tcattatcta tcaaatctag
    11341 taacgggtct ctaaatatat gaagtgatgt gtttttcatt gatgtagaaa ttcccacaaa
    11401 actcattccc ttacttaaat tttttatcca tgttttctca ttttaagttt taaaaatgcc
    11461 aaagcttttt tagcatctat ttcatttgtg ttgttttctc caactacttc tgtctcaata
    11521 ccttcttttg atttgttcat atcaactatg gtatccatat attgattgct tacaacaaac
    11581 ttagcttgag tcccattaaa tgtaactcca actacaggta tatctctttt tcctatttcc
    11641 tcaaatgtgt tagcgtagtc aacatgtgag tttccccaac catctgatga aactatagct
    11701 ccatctactc tcatagcttc cagccaagcg gccgttcttt gtcccacaaa ggttttattt
    11761 tcatttccat ctggagttcc tactactatt actccaagta aatctacatc cttatcactt
    11821 tcaactatgt ctaaaagtgg gtctctaaag tgatgaagag atgtttcttt tgttgaaggt
    11881 cctattccca taatatccac ctctcttaag tcatagctct aagggcacca tctctgtatt
    11941 cattaggaga tataatcata ggtacatttc ccatatctat aattgatgtt cctccttcaa
    12001 atccactagg ttcctttgag aataactgat tgtcatacat agcaccttga cctgctattt
    12061 gttttactat gacaaccttt tttgcatttg gtcgaatttt gtcaaaatat tcacgaactt
    12121 gagtagcatt tcttccgtct tccattttca aagacgctct tatttcttgt ataaaatcat
    12181 cacatgctct gaatgctgca agtggaagtg ttctttcaaa aggtaaacct ccctttaaag
    12241 ttacatctac atggattata taatcttcaa ctgatggagt tccatatctc ccaaatacca
    12301 tttggtcttt caaaattcct tctgaagaac caaattcatg catttgattt ccatcttcat
    12361 ctgcaccagt taacattaca tatacacccg ttaaagtatg tgtaataccc tctcccagtc
    12421 tgcctaaaac ttttgttgat attggtataa tgtccataat agtattaatt tctctgtcat
    12481 aatctccagg ttttataatg tctactttta tgtctgttat taactcttct ctttctttta
    12541 tttcatctat acttgataaa tctattgtaa gtacatcttt ctttatagaa gtttttgaac
    12601 cgaaattaac ttcattcata tgaaacggtt ttataacaag tcttctcatt attttttctt
    12661 ccataatatc cacctttgtt cttaaaaccc ctctcctaac cgtgtaggag aagggttcta
    12721 tattttcaaa tataaattac attttaaacg tgagctttat attcgtatgg taatggaact
    12781 atcttaccag cagatggtat tgcaactaat tgctctaatg tatctcttaa tatatgtgtt
    12841 tgcatttcta tattatgtgg ttcaccagca ttagcaccca ttggaactaa tggagcaact
    12901 gctctaggag ttccgttttg tctaactact ggaggaagag ctgctattat tattgtaggt
    12961 attccagctt cctctattgc tctctgcacg atcacggcag ttctatggca agtacctcag
    13021 ccagctgtca taactacacc gtctactccc tcttccttaa gtttagcagc gatagcagga
    13081 ccagtttctt cagtgaagac atgttggttt cctccaccac ccatgaatgc atagtgcata
    13141 ggagctactc ctttgataaa tccttctgca gctaattcat gtaatctatc tatagggaac
    13201 atacagttta tatctttgtt tacatctcca ttgtcatatc caccatgtga taccatcatt
    13261 tcatcaactg tagctgtatt aggtatagct ctaaatgtag tatctcctgc tagattaaat
    13321 ctcttgtcag attttaaatg aacaccagct gctgtagcta aagctatagt catatctttt
    13381 aattctttag ttacaggagt ccaaactgga ggaggtgtta ttggaacgaa tatttcagat
    13441 tgaagtcctt gtactgttgt aaggctcata tatttcctct cccttctatt ttcaaacgat
    13501 tatttattat ttttcttctt gttttgattg catttctgct tgtttttctt tttctctcct
    13561 aatattttct acatactctt gattaggctc tggacttaat acctcaggat agcttagcat
    13621 gaatccaact tcttgaccta tcttaagttc gtagtcagtt ataagcattc tattaggtat
    13681 tttaaattgt ccaagtctac cttttaggtc tatagtcact ccaccgcctt tgatgtcaac
    13741 tataatacct tcataaaaag tctctgatga gatatatttt aacctatcca ttataattct
    13801 acctctctat actgttttaa acttggatct aaatttatgt tattgtctaa atcttagtta
    13861 gtatctaaat tttagttttc gtctttttcg tatatttctc ttctcttctt actcattggt
    13921 agagattgct cattctcttc taatactatt ttttctcctg taactttttc tatagcttct
    13981 atgttattta atttaacatt tggattccat tttctttcag gagctttaac ttctacacct
    14041 tctatagcat ttttaagcat agccattatt ctaacagcat cttctggagc taaagtgttg
    14101 ttagataata tttcattctc tataccttgc ttagatttat tgttgtctac catgtgagtc
    14161 atgtatttat taccaacaac tagagcacct tgaacagctg agaaacttac accaactact
    14221 ggtatacctc tcatacctat ttgctctatg tgagatgcga aatctatatg gttgtttccg
    14281 aaaccttcag tagttactac agctccatca acttccatag cttcaactaa catacctaat
    14341 ctcttagata catagaattt ctcagcattt acttgtggag aaccaacaaa tacaacacct
    14401 actaaatcta tttcttcgtc ttccatagct cttattacta atggctctct ccagtaatgt
    14461 cttgacattt cttttgatgc aggtcctata caagttaatg catggatacc accatctaat
    14521 acttcaagtg gagataatac aactggcatg tttcctaagt caacgtttgg ttgagctcct
    14581 aatgtaccaa ctggctcaac tggtaatatt aggttatcat gcattgcacc ttgtcccatt
    14641 atttctttta taacaacaac tttcttttta ccttctcttc tgtattgctc tattacttct
    14701 tcatcaacta ctaaagagtt gtcaactttc tttaatgctt ctcttatttc ttgagttaca
    14761 tagtcaaatg cacggtgagc agctaaaggt cctggtcttt ccatgttagt tcctgctttt
    14821 attgttactt gacctttgat gaatatttca cctttgtcag cagcacctgg tctaccccac
    14881 attatagtag tatttaactc accttctgaa gaaccaaatt cacctatttg aactccatct
    14941 tcatctgtac cagttaatac cattacagct ccatctataa ctctagttat acctgaacct
    15001 aattcgcctt cttctttagt agcgattggt tgtatatcta atacagcttc actgtaagtt
    15061 tcatatttat caggagttat tatttctaac ttcatatcta caactaactc ttgagtttca
    15121 ttagcttctt tacatatttc ttctgcgttt ctgatgtata aagtagttcc ttctatttta
    15181 gtttcatcag caaattcaac tttatctatt ttaaagtgtt ttttagttaa gcttcttaaa
    15241 agtttctttt cttcaacttt tggagctact tctgctgctg cacttgccac tggagcagca
    15301 acgtttgcta ctggagcagc aactcctgct tgaccagcta ctgataaagg tatctctaag
    15361 cttatgttct taccttcacc tatgtgtatt ttaactgttt cacctgcaac tcctgtgctt
    15421 actggaacta ctgctgcttc tgatactgga gctgcttctt ctactgtttc ttctactact
    15481 tcttctttag cctctcctgc tttgtatcct tctacgttat ctgtagtcat tggagaaagt
    15541 gcatctaaag tttcttttaa cttagctcct agtacttgac ctatagttaa tccattttct
    15601 ggtattgtta ataatccaga atcctctaag tctgcaaata ttgctggatc ttctaagttt
    15661 tctggcgcta taattgttcc cgcttcaaat ctacaacaac aaactgctgg gtcatttgca
    15721 tgggcttgag ctgtttctaa agttattgac atagtataaa atacctcctt acattatttt
    15781 cttatttatt attttaaatc tcaattcctc aacaggtaac cccttaactg agaaattaag
    15841 taaattaaaa cctacataat ttgttatgat taatattatt tttttctcac ttaaagtcac
    15901 tttatattat aagcaattcc tatgccaact tattaacaaa aagatttttt ctataataga
    15961 ttcttcatct atcgctattt ttagccaaat attatataaa tatttattta aaatatgtta
    16021 ttttttgtat ttgtaaacat tttctcaaac aaaaaaagtg tcaattaatc aacacttttt
    16081 catttttttt aacacatcta tatacacaac acttatataa gcgttggctt tactaggttt
    16141 taaattaaag tttatcattt tgtcaaaatt tagtcatgtg tcatatttta gacacatcta
    16201 ttttgtattg gtctatctta taatacaaag ttgttctggg tatatttaat atcttagctg
    16261 ctttagcttt atttccatta gacattttaa gtgccttggt tatattcttt atttcaattt
    16321 tttgagttgc cttcgttaaa tctaaaggat actcttcttc ttcagtacta ttatttgtag
    16381 aatccatcat ataacttggt acatcatcca catctatttt agaattttgt gacaatacaa
    16441 caatattttc aatcgtattt tttaattctc taatattacc tttccatcta taattctgaa
    16501 atatatctat tacatctttg ctaattgtaa gaacaggctt attattttgt ttacatatct
    16561 cctctaaaaa gctatgaacc aataatccta tgtcttcttt tctctctcta agtggaggaa
    16621 tctttatttc aacaacattt aatctatagt ataaatcttc tctaaatttt tctgccttta
    16681 ccattttact caaatcttta ttggtagctg atattattct aggatttatt tttattgtgg
    16741 tatctccacc aactcttctt atttcttttt cctgtaatac tctaagcaac tttgcttgca
    16801 tactaagagg taaatcggca atttcatcta aaaatacagt tccatctttt gcaagttcaa
    16861 atatacctat tcgacccttt ttacttgctc ctgtaaatgc acctgattca tacccaaaaa
    16921 actcactttc aaataactca tttggtatgg cactacagtt aacaggtata aattgccctt
    16981 ttctctcact ataatcatga atagctcttg caaatacttc tttacctgta ccactttcgc
    17041 cccaaataaa tatactacta tttgttctag ccacttgttt tgcaatcgct ttcgattttt
    17101 ccaatttata acttttacct aaaatcttat caaaactacc tttagataaa tttttgactt
    17161 cgttctttag atattttaat gtatcattta ctttttcaag ttcaagagct aatatcttag
    17221 cttctgtaac atcaacttct gtacatacaa caccgataaa ttcttcttta taaaatatag
    17281 gatttgcatg aactaatgca tacatatttt cttttttatc tgtaaaatac aagtcactca
    17341 ttccaacttt actatttagt actttttctg aaatagtatt ttccaaaaat tcattcatcg
    17401 gtcgaccaac tatatcttca gactttatat catacctttc ctccataaac ttattccaca
    17461 agataacaac accttcgtta tttatagcac aaattccttc tttaatttga cctataatat
    17521 acttcaaagt caatccataa tcttctaaat gcatatatag atagtcccta atgtgctctt
    17581 gttttagaac tccaataatt ttattatctc taagaacagg aagaatccct atatttttta
    17641 atatcattat atctctacat tcatctaagg ttaaaccttc gttgacatat attacatcct
    17701 tcttcattat aagttttact ggttgacctt catacttttt atgcatctca taaagattat
    17761 gtatatccgt catggatata atgcctttta attggtcaga tgaatctatt accattagag
    17821 tttttgtgtt gctttttatc atttctttta ttgcatcttc aattcttgtg tcttcgtcta
    17881 tagttgtaaa ttttgtatcc attacctctt ctactttttt tacttctggt atcgaaaaca
    17941 taaagtcccc cctttatagt agtacctcat caaaacttat ttatatatat ttattctagc
    18001 atattataat aattatttat taaataatta ttattaaaat ttattaagta attagcattt
    18061 aaatttatat aatcacttta aaatttataa ttaaatttat acaaatgaaa tcaaatgaat
    18121 tataccctta gtatattatt ttttcaatac tattttcacc ttcaaaacat ttttctattc
    18181 atcaaacaat taatgaatgc ctgtatc
//
LOCUS       DAFKMX010000078        17993 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFKMX010000078 DAFKMX010000000
VERSION     DAFKMX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.078"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(258..929)
                     /locus_tag="LZB08_002029"
                     /pseudo
     CDS             complement(258..929)
                     /locus_tag="LZB08_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
     gene            complement(956..1678)
                     /locus_tag="LZB08_002030"
     CDS             complement(956..1678)
                     /locus_tag="LZB08_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBM7315533.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(1678..2007)
                     /locus_tag="LZB08_002031"
     CDS             complement(1678..2007)
                     /locus_tag="LZB08_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBM7315534.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            2337..3263
                     /gene="fba"
                     /locus_tag="LZB08_002032"
     CDS             2337..3263
                     /gene="fba"
                     /locus_tag="LZB08_002032"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBM7315535.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(3398..5029)
                     /locus_tag="LZB08_002033"
     CDS             complement(3398..5029)
                     /locus_tag="LZB08_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315536.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(5512..6549)
                     /locus_tag="LZB08_002034"
     CDS             complement(5512..6549)
                     /locus_tag="LZB08_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBM7315537.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(6568..7353)
                     /locus_tag="LZB08_002035"
     CDS             complement(6568..7353)
                     /locus_tag="LZB08_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBM7315538.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7386..8519)
                     /locus_tag="LZB08_002036"
     CDS             complement(7386..8519)
                     /locus_tag="LZB08_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBM7315539.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(8567..9694)
                     /gene="hadC"
                     /locus_tag="LZB08_002037"
     CDS             complement(8567..9694)
                     /gene="hadC"
                     /locus_tag="LZB08_002037"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBM7315540.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(9694..10920)
                     /gene="hadB"
                     /locus_tag="LZB08_002038"
     CDS             complement(9694..10920)
                     /gene="hadB"
                     /locus_tag="LZB08_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBM7315541.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(10935..11735)
                     /gene="hadI"
                     /locus_tag="LZB08_002039"
     CDS             complement(10935..11735)
                     /gene="hadI"
                     /locus_tag="LZB08_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBM7315542.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(11774..12973)
                     /gene="hadA"
                     /locus_tag="LZB08_002040"
     CDS             complement(11774..12973)
                     /gene="hadA"
                     /locus_tag="LZB08_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBM7315543.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            13650..14648
                     /locus_tag="LZB08_002041"
     CDS             13650..14648
                     /locus_tag="LZB08_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBM7315544.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15010..15537
                     /locus_tag="LZB08_002042"
     CDS             15010..15537
                     /locus_tag="LZB08_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBM7315545.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            15564..16160
                     /locus_tag="LZB08_002043"
     CDS             15564..16160
                     /locus_tag="LZB08_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBM7315546.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIDVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16225..17808)
                     /gene="asnB"
                     /locus_tag="LZB08_002044"
     CDS             complement(16225..17808)
                     /gene="asnB"
                     /locus_tag="LZB08_002044"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBM7315547.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     YERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDITSRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5532 a         3255 c         2250 g         6956 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacgagtg
      241 tgcgtagcta ttttttaatc aaaatattct tgagaagaag ttactatatt aaccataatc
      301 tcattagtat gaatcccttt tctaactaca agatgtctca aatatccttt gtgattcatt
      361 gttctataat aaggaagctt tttttcattg aaaaactcta cactagaaac taatatcttt
      421 gagaagtctt catcaaccaa catacactcc tcaacagttt gtatatctat atgctttcct
      481 tttttatgaa gacctaatgt caaaggtcca ttttttactt catctccaaa agtatattcc
      541 atcttatttc tataatattt gttttctgga cttccttcta tccctaaaaa ctgaaatcca
      601 aataaatctt gctttaaaaa taaatccata acttgttttt cttttatttc taattgtttt
      661 tcatatggca cagacaatag tgtacatcct ccacactctc taaagtgctt gcaagtttct
      721 tcagtttcaa gtggtgagtt ctctaataat tctatcatct taacttcagc tttcttactt
      781 ctaacttttt taactccagc tctcaccttt tgaccactta ttccaccctt catatggata
      841 actctatctc caacttggct taaagatgta ccaccaaatt ccattttatc tacttcaaat
      901 tctataacat cttttttctt cacagacatt cctccaattt actatagtat ctttattatc
      961 tttcttcaat caattttact tcttcatctg ttaactctct atactcacct aactctaaac
     1021 tttcatccaa ttttaaattg cccatagaca atctttttaa gtatactact tttttgccta
     1081 cgctttcaaa cattcttttt acttggtgaa acttaccttc atgtattgta agctctattt
     1141 ctgattcatc atcacttttt aatatattta actgtgatgg catagtttta tatccgtcat
     1201 caagcactac accttccaaa aaagcttcta catcttcttc agtaactact ccatcaattt
     1261 ttgcataata agtcttaggc acatgcttct taggagataa aactctatga gaaagctttc
     1321 catcattagt taaaactaat aatccctctg tgtctttatc taaccttcca acaggaaatg
     1381 gttcaaaagc taagtatgat aaatcaatta aatctaatac tgttgggtca tatttatctg
     1441 tagttgctga tatatatcca tcaggtttat tcatcatcaa atatatatat tctctatata
     1501 taacttcttc tccattaaat aatatctcat cattttcagt gtctacttga gttcctggat
     1561 ttgaaacttc acttccattt actactacag acccttgttt acaatacttc tttatttcac
     1621 ttctgcttcc ataacctaaa tttgaaagaa ttttatctat acgttgcttt ttagccatta
     1681 aaaatgctct ccattctcat aatatttttc tttttcaaat actgtaaatg ctaaatccat
     1741 actttctaca tctaaattat ttcttatact atcagttatt attcttgcta ctatatcttg
     1801 tacttcttgt cctctatcaa accaagctac ttcaacaaaa ggatatacat ctgctatttt
     1861 accatctctt attgcaactg actttataca ttctatttca aagtagtctc ttggacattt
     1921 tacagcttca actaaatcat ttatcatttt ttcacttatt ttacatatat cattttcatt
     1981 aattcctctt atttttatct gtggcatagt ttatctcctt tattttaaat ttttagtgaa
     2041 ttatcatcca cttaattccc ttcgataacg tttgaagtgg taacttcttg gtcaagacaa
     2101 ctattgttat cagtatctcc aaactaataa ggtagttcct acccttatag ttagcattat
     2161 atagctaatt ttcctaaatt gtttatattc tttattattt tatttaatcc tattgtcttc
     2221 tatttaacat tatacttgta atttatagtt attacaattt actatatact ttttttgatt
     2281 taacgttata atataagtga tgggtaaatt ttaaaagtta ataggaggag tttaaaatgg
     2341 cattaattac tacaaaagaa atgtttaaaa aagcttacga aggtggattt gctataggtg
     2401 catttaacat aagtgactta gaacaattac aaggtgtttt aaaagctgct aaagctaaaa
     2461 attcttatgt tatgatacaa gcttctatgt cagctgttaa atatgctgga cctcatacat
     2521 tagttgaaat ggttaaggct gcttctgatg aaataggtgt agatgtagct cttcacttag
     2581 accatggtcc aaatatggat gctattaaaa cttgtattga tgctggtttc tcttcagtta
     2641 tgatagatgg ttctcatttt gactttgaag aaaatgttag aataactaaa gaagctgttg
     2701 aatatgcaca ctctaaaggt gttgttgttg aagcagaact tggtgttcta gctggaacag
     2761 aagatgatgt tacttctgat gttcacaagt atactcaacc agctgaggca gttgagttcg
     2821 ttgaaagaac aggtgttgat tcattagcaa tagctattgg aacttctcat ggtgctttca
     2881 aattcaaagg agaagctaaa ttaagattcg atatactaga agaaatccaa agcaaattac
     2941 caggtttccc aatagttctt catggtgcat ctgctgttga ccaaaacgct gttgctacat
     3001 gtaatgaatt tggtggaaat atagctggtg ctaaaggtgt acctgtagat atgttaagaa
     3061 aagcatcttc tatggctgtt tgtaaaataa acatggatac tgacttaaga ctagctatga
     3121 ctgctgctat cagaaaattc ttagctgaaa atccaaaaga atttgaccca agaaaataca
     3181 ttggtgctgg tagagatgct atacaagctg tagttgaaag caagatagat gatgttttag
     3241 gttctgctaa ctctataaac taatataact tttaaatatt tataaaagaa aagagatgac
     3301 ttaaagtaat taaatttatt tttaagccat ctcttttaag ttgtatcaat tatgtatctt
     3361 ataatatttt aattatattg tctttttaaa ctgacagcta aacgtgaata tgtccactct
     3421 ttatctttct atggatggta gaaggatata tacctataac ttcagcagcc tttgttattg
     3481 aaccatactt atttatagcc ttacttatca ttatttggtc cactatttta tatgcatcat
     3541 taagattcat aattccattt accactattg gtgataaatc atctgtttca ttatcaatca
     3601 tatctaaatt tataagcatg ttaaattcat cttctccaat tacattttta gctgataata
     3661 ctacaaatct ttcaatgatg ttttttaatt ctcttatgtt tcctggccaa gaatattctt
     3721 ctaataactc aataacttta ggcgaaactt ctacttctct attgtacttt tcattgtaaa
     3781 gttttaagaa ataatttact aatggtacta tatcctcttt tctttctcta agaggaggta
     3841 tttttactgg aataacactt aatctatagt acaaatcttg tctaaattta aggttatttc
     3901 taaggtcttc atgagaaata ttagtagcac ttatatatct aacatctatt tttataggct
     3961 tactaccacc tattctagta atagtatttt cttgtataac cctaagtaat tttttttgca
     4021 tctgcattgg taactcacct atctcatcca agaaaatagt tccaccattc gcctcttcaa
     4081 aaagtccctt tttacctttt ttcttagctc cagtaaaaga accttcttca tatccaaaaa
     4141 actctgactc taataattca tttggtatag ctccacagtt tatagctata aaaggtttgt
     4201 ctctccttga actatactta tatattgttc ttgctattat ttcttttccc acacctgatt
     4261 ctccatttat aaatatagaa gaatctgagg ctgcagctct cttaacaact ttttttatat
     4321 tgtccataat cttactttga cttatatact ctgtgtcttc tgatatatca ctatatttta
     4381 tctgagattc aagcttaata acccttgaaa tatctctccc tgttagtact cctcctgtaa
     4441 gctttccctt agaatcccat cttggaactg ccgtatatgc aataagcttc ccatctgggt
     4501 aaacgatatt tctataaatc tttttcttca ctttaagcat gtttaatatt attggttctt
     4561 caactaatcc ttgtctcaca aggtctgtta tatttctacc aagtacctca gttcttttaa
     4621 aaggtaatat ctcatcactg acagtgttca tcttttcaat taccccatct ttgttaaaca
     4681 caacaacaac ttcatctata tgctctatta tatcttcaat tactttatcc gatattagag
     4741 acttctcaaa taaataaact atattatttt cattattatt atctttcata ttgtatatat
     4801 gtgcagtcat ccttaactct ctgaaagcta ttttcttttt actatctgtc agtatttctg
     4861 ttttgtcaaa atttgaatct aattcattta tatttctagg cttttttata tctagaaaac
     4921 taaaaatact atctattaga tgattacagt atattatttc gcctgattct ttacataaaa
     4981 tggctggatt ttctacatgt tctataagcc ctattaaatt attatccatt aatattcgca
     5041 cctttcatta agtaatttat tgaactacat acaaaattta ttgtttgctt acaataaata
     5101 ccatcagcac atataatcaa ctccttataa tattcgtctt ttcaagtagt ttatttcatt
     5161 aaaacgtaac taagatataa ttttaatgat tatagtttgt taaaaatttc attttgcttt
     5221 taattgtatt ttatcacata tttagtaatt atttgtctat ttaatacaat ttaattaaat
     5281 ttaagattta tttaaaaaaa caaagataat ccttacagaa tataactcac aatttgtaag
     5341 caatatacat ataaggatta tccagattat cttgtattat aaaattttat tcaaacagac
     5401 tttttaataa ctgacaaata cacactagca ataacttatc aaagggttaa taattatgtt
     5461 actaactaat aagacttatt caatcagtaa tatttatata gtttgtatct attaagcttt
     5521 tatagcttct aataaatcat caacgctatc taatttttct ataagcattg gaactatttc
     5581 atgtaagtct ccaactactc catagtcagc gatttctaaa attggagctg ctgggttttt
     5641 gtttatagca attatgaaat ctgatgattg catacctgct aaatgttgta ttgctcctga
     5701 tataccacaa gctatatata ggtttggttt aacagtagtt cctgtttgac ctacttggtg
     5761 agaatggtct atccatccag catcaacagc cgcacgagaa gaaccaacta ctccacctaa
     5821 tttgtcagct aatttcttaa gcatttcaaa tccttctggt ccacctaatc ctaaaccacc
     5881 tgatactata acatttgcat ctgtaagaga tactaaatct ttcttagttt taactgtttc
     5941 taaaacagta gttcttatat catcttgagt tattttgtag tctaatgcta taacttcacc
     6001 agttcttgtt tcgtcttttt cagctttatc cataacacca ggtctaactg tagacatttg
     6061 aggtctatgg tttggacaaa tgattgtagc cattatgttt ccaccaaatg ctggacgagt
     6121 ttgttttatt ttcttatctt ctgggtctat ttctaactta gtacagtcag ctgttaatcc
     6181 agttccaact cttgaagcta ttctaggtgc taagtctcta cctatatgag ttgctccgaa
     6241 aagcattatt tctggtttta tttcatctat tgcatctttt attacttttg tatatgcatc
     6301 agttgtatat ttttctaaaa gagcatcatc tgcaacataa actttgtcag ctccaaaagt
     6361 gattaattct ttagctaatc catcaacatc ttttcctaat aatattgcac aaagttctgc
     6421 atctacttct ttagctaatt ttcttccttc tcctaataat tctataacta ctggagctat
     6481 ttttccttct ctttgttctg caaatatcca tacgttttta taagaactta aatcttttat
     6541 atcattcata gtttgtactc ctccaactta tataatgtgc ttttgtttta gaccgattat
     6601 tattttacta gctatttcat cagctttacc ttctaagatt tctccttttc ctcttggttc
     6661 aggagtaaat gatctaaata ctttagttgg tgatgctttt aaaccaactt cttctttatt
     6721 tacatctaaa tcatcaattg tccaaacttt tacatcatgt ttatatgctt ttactatttt
     6781 atcaacagac atgtatctag gtgtatttaa ctcttttact gctgttaaaa gtacaggtgt
     6841 acttactttt attaattcgt atccatcttc taattgtctt tgaactatta tatcttttcc
     6901 ttctattttg aaatcttgta catatgtaac ttgaggaatg tctaatttct ctgcaatttg
     6961 tggtccaact tgtgcagtat ctccatctat tgcttgtctt cctgcaaata ttatgtcata
     7021 gtcacctact ttagatattc ccgctgcaat agtatttgat gtagcccaag tatcagctcc
     7081 tccaaatgct ctatcactta ataatattgc atcatcagca cccatagcta aacactctct
     7141 tagcataaag tctgcttgag gaggtcccat tgttataact gttatagtta catcatcatg
     7201 ctcatcttta atttttaaag cttcctctaa tgcatttgca tcatctgggt ttaatatact
     7261 tggaactcca tctcttataa gagtacctgt ttctttattt attcttactt cgttagtatc
     7321 tggaacttgt tttacgcaaa ctaatatttt caaagtctat acctccaatt tcattagatt
     7381 atttattatt ttaatacgtt tgatgaaatt accatttttt gaacttctga tgttccttcg
     7441 tatattgata cgattctagc atctctgtac attctttcga tttcataatc ttttataaat
     7501 ccatatccac cgtgaagttg taatgcttta taagcaactt catttgcaat ttctgatgca
     7561 tagtatttag ccatagcaga ttcttgagca actctcattc ctgcatctct cttttgtgct
     7621 gcatcataaa ctaatcctct agctgcacaa acttttgttt ccatgtcagc tatagtaaat
     7681 tgagtatttt ggaatttagc tataggctta ccaaattgaa ctctttgttt tgtatattta
     7741 acagcttcat ctaaagcacc ttgagctatt cctagagcta aggcagcaac acctattcta
     7801 ccaacttcta gagtacccat tgctatctta aatccttgac cttcttttcc aagtaagttt
     7861 tcttttggaa ctttaacgtt ttcaaatatt aagtcagaag tttcagttcc tcttataccc
     7921 atcttatctt catgagctcc tgttgagaaa ccatcccatt tactttctac tataaacgct
     7981 gatgttcctc taagtccttt acttctatct gtaactgcta ttactatagc tacatcacag
     8041 aatggtccat tagttatgaa agtttttcta ccatttaata tatagtggtc tccaactaat
     8101 tctgctgttg tttgttgtcc acctgcatca gaaccagctc ctggctcagt taaaccaaat
     8161 gctcctatta attcacctgt tgctatacct tttaagtatt tttgtttttg ctcttcagta
     8221 ccattgtaaa gtattggtaa tgaagcaaga gaagaaccag ctgtaacaaa agtagctgtt
     8281 gaagcacatc ttctagctat ttcttccatt actatagcgt aagaaacata tcctgctccc
     8341 ccaccaccat attcttctgg gatattactg cttataaatt ttgcatcagc taattttttg
     8401 attaattcct taggcattac acctgtttta tccatttctg ctggtaaagt atctaattct
     8461 ttactaacaa aatctcttac tgcttttctt aaaagttctt gttctttgtt atataacata
     8521 gcttcctcca attttcatat tttatagtaa atttatttta atgaacttac gctaaactca
     8581 tcatctcagc aaaagtttga atacgagttc tggcttgttc attattctga gtttgttggt
     8641 cgatttcaac atataaagta ggtattccac tatcttctat atcttttcta actaaaggat
     8701 aatcgtattc ttctgggtca cagaatttca tcatacagaa gataacacca tctatatctt
     8761 tctttttaac ttcatctact ataagtgacc cacgtttttt cttagggtca taagctaatg
     8821 aacatccttc tatgtttgac cattgtcttg ctaatctctc taacgcatca tcacctgctg
     8881 gtacatctgt tctgaattgt cttgtttctt gtgctaagtc gtcagctaca actgatatat
     8941 tgttgtcttc taaaatgtct aatatatctt tagaatcagc taatatacct gttaataaaa
     9001 ctttctttcc agaacagact tcttctggca tagcatttaa ttttgctatt aaatctttca
     9061 ctagctcagt atgttcttct tttctcataa agaacccact cttaattact aagcttctta
     9121 ttgatggttt tatagtatta gaatgtttat aagctacttc aacaaagtct ctcatagttt
     9181 ttctatgttc attgtaaact tctatacttt catgaatttt tgcttcttct atttcatatc
     9241 cacaaatttc ttcaagttct ctttttacgc ctttgtactc actgattaag tattttacac
     9301 cagcttcaag ttttctattt tgtgggtgta ctaatgatat atattttata tgaggtactg
     9361 ctgatttcca gttttgtcct aaacaaatta gtgtatcaca catacccggt ataataactc
     9421 cagataattc atcataagca ccttttaatc catattctaa acatgattgc attattgaac
     9481 atgcaaatgc agggaaatat tgtttagcta aatctaattc tgtttgtcct ccccatatac
     9541 caactggaag cattccagct gcatgtataa tttcttctgg gcaataaact gggaaacaac
     9601 ctatagcttt tttaccagtt tcacttttat atttttttac agccgcattt ggattttcaa
     9661 ctacttcttt cattttagat aaaatagctt ccattatatc tcacctctat taagtttttt
     9721 tctttcttcc attacttcaa ctaaaccttg aattcttgtt tcaaattgag cattagtgaa
     9781 agctctaggg tctgcttggt caccatcaaa tccagcatat ggtaatccag tttcttcagc
     9841 tgctcttctt tgcatttcat attgtattaa actcataagt ttacagcttc tgttcatatg
     9901 atagaatgct ccatcacatt taccctctac taaagaatca actctatatt ttgtcatacg
     9961 gtctaggttt acattgttaa acatagtact atatgctact gccattccat ctaaatcatt
    10021 aacttcatat tgtaatgccc aagcatgtgg gtaaacacta cctgtcatgt taactccaaa
    10081 tttagctaat gttttcatct tgtatcctat atatggccaa caaggtatac cttccatcat
    10141 tattctgtat ttttcttccc ctctgaaaga agatttacca gttttcatgt tgtcctctaa
    10201 ttcttctata agtaacttaa atgcttctgt agtttctttt ttacctctag cacaaactat
    10261 tacagccatg taagtaaata agtcaaatcc attcattgga gaaggagaag aatctgctgg
    10321 taaactcata gaatacttcc atagtcttcc gttttcagct gatattttca ttacttcttc
    10381 aaacttctta gggtcaaatt tctttcctga tataatttct agttgtttta tagcttcttc
    10441 aaattgagct ttaatataat ctattcttga ttgagtaact tcgtcttcat tattgaaagt
    10501 tgtatcaatc attattaaag gtatatctaa ttctcttgaa atattttcat accattttat
    10561 aacttggtta catatattgt tacagcaaag taggaaatct ggagccggca tatccaaagc
    10621 ttcacatcca ccattttcta aaagaccaaa atttgttctt gcatatgcac ataggtcaat
    10681 agaatatcct ttagattcag ctatttcaca taattctaaa gaaccttttt tagctgctac
    10741 tccagctgct tggttttctg gatataatac gtttaagtca aatacttctg ctaactcttg
    10801 aggaaatact gatgttgacc aacctacagg tcttccttct tctttagctt taaatgcatt
    10861 tgcatattgt tcagctaata aatcattaat tactactcta gcttcttttt tttcagacat
    10921 tttcatccct ctttttatat atttttcact tctttttgtg attcttttgc ttcatcaaaa
    10981 gcatataacg ctgctcctaa agcaccagtt aattgaggta tatcaggtac aataatttct
    11041 gtgttgattt ctcttgccat agctcttaca ataccactat ttctagcaac tccaccaacc
    11101 ataactacat ttctttgtac tcctattctt tttactaggc tagaaactct ctttgctact
    11161 gaagtatgaa tacctgccac tatatcttca atttttgcat tttcagataa atgtgatata
    11221 acctcagact ctgcaaatac tgtacatgta ctgcttattg atacttcatt ttgagaattc
    11281 atagatatac ttccgagttc agatacatca acctctatta tttttgccat tacatctaaa
    11341 aatcttcctg tacctgcagc acatttgtca ttcataagaa agtttaatag tcttccatta
    11401 ttatctaatt ttaatacctt tgcatcttga ccacctatgt caataatggt tcttgtctct
    11461 ggaattatga aatttacccc tctagcatga cagcttaatt cacttatttg cttatcagca
    11521 tctgaatagt tcattcttcc atatcctgta actacaactt ttttaatatc ttctcttgca
    11581 agacctgtct tgccatataa tttttctaaa actcttgatg gtccagtagt cccagtacca
    11641 gaggatattg tttcagaagc tacaatatct tccccattct ttaagattac tccctttgat
    11701 gcagttgaac ctatatctaa tcccattgtg tacatcttta gcattctcct tttaaaaaat
    11761 ctagaattac taattaatat cttacaactt tactatcttt aaagttattt attttctctt
    11821 catcgtagcc taaagatttt aatacttcta cagtatgttg acctactttt ggtgctggtg
    11881 tatattcttt aattccttca tttctaaaca taactggagt attaactaag acacctgtat
    11941 ttccgctatc gtatgttttc ttaaataaga agtcatttgc ccaagcttgt tcgtcatcta
    12001 ataaatcttc acagctttga attttttcaa atggtaagtc tgcttcttct aataaagctg
    12061 accactcgtc taatgttttt tctaacatag cttctccaac tatcttaact aaatcttcaa
    12121 catgattaac cattgaatct atgttattat atctatcatc ttctaatata tattctctat
    12181 ttataacctt acagaatttg cctaaccact tgttgtattg tattaaagct aattgaatcc
    12241 atcttccatc tttacattta tatgtagtca ttaatgggct gtttggattt tctcttgata
    12301 aaggcatttc atttccgtat tgtgctgttg ttatcattgt tcccattcca tatatagctg
    12361 tatggaaaag acttactgtt actctctcac ctttaccagt ttgagctttt ttatgtaatg
    12421 ctgctaaact tcctgctgct agtgctagac ctgcatagtg gtcaccaaat cctgctgctg
    12481 tatttgctgg agatgttcct ttttccataa cagattggct aactcctcct cttgcgaagt
    12541 atgcagtata gtcaaatcct ggtttatctt ttaaaggtcc tttttcacca tatcctaata
    12601 tttgagagaa tattaatcct ggatacttat cttttatttg gtcataagct atacccattt
    12661 tttctaatgc ttgaactcta acattagtta caaatatgtc tgcttctgat aataatttat
    12721 gtaatatttc tactccttct tttgatttta cattaatact tacacccttt ttatttccat
    12781 tttctaattc aaacataggg ttttcatcat ctgatgctgg agatttaaat gttccaccca
    12841 taactcttat tccatcacct tctataggtt caatcttaat aacctctgca ccccagtcac
    12901 ctaacatttt tgcacaacat ggtgctgcga tgaaacttga aagttctact actttaactc
    12961 cttctaaaag cataacgttt tcctccttaa tagtatcaat tataaaattt cgattaaatc
    13021 actcataaaa tacttttttc tgatattaac tgtctgatgt caaagttcct aatcttatct
    13081 aaaataactt aaaaattatt aaaattattt tttattgttt tattgtctta cacctatatt
    13141 gttaagcatt tatcgtgcca actttatatt taatcaaaat atcgcttgat ttgaatgttt
    13201 tttaacaata tgcaattgaa taatcttttt catatcgtgg catcttgcca ctatccattg
    13261 cattttgcta ttctttctat gcttataaaa gtttttttta tatatttttt tatgaaaaag
    13321 cttgacattt caaaaaaagt tttcattgct aaactcaaaa acacatgtta tcattgtaat
    13381 taatcagcaa gttagttaac taatttcaaa cttagataat tttttaataa tatttaatct
    13441 aaaaaataac aatttctttg ataaatttta gagtaattat tacattaaat aaaaaattac
    13501 taaatatcaa aataacacag ggaaaacaat ttcaaaacta aatttttaac cttaaaaatt
    13561 ttgtataaaa aatataacct tatatgctac atattatttc taaatgaaaa attaccataa
    13621 taatttaata attacaggag ggtattgata tgaaaatact agtatttgga gcacgcgatt
    13681 atgaagaacc agtaataaaa aaatggtctg aagaacataa ggatgttcaa gtggatattt
    13741 atcctgaaaa catgactgaa gaaaatgtag ttaaagctaa agggtatgat ggtatatcta
    13801 tacaacaaac taactatata gataatcctt atatttatga aactttaaaa gatgctgggg
    13861 ttaaagttat agcttcaaga actgcagggg ttgacatgat acattttgat ttagttaatg
    13921 aaaatggact tatcgttaca aacgttcctt cttattcacc taatgcaata gctgaattag
    13981 ctgttactca agctatgaac cttttaagaa agactcctct agtaaagaaa aaagtctgtg
    14041 aaggtgatta ccgttggata gctgaacttc ttggaacaga agttagatct attacagttg
    14101 gtgttatagg tacaggaaaa ataggtgcta cttctgcaaa attattcaaa ggcctaggag
    14161 ctaatgtaat tgcatttgac caatatccaa atagtgattt aaatgatata ttaacttaca
    14221 aagattcttt agaagacctt ctaaaagaag ctgaccttat aacattacat actcctttac
    14281 ttgaaggaac aaaacatatg ataaataaag atactctagc tataatgaag gatggagctt
    14341 acatagtaaa tactggccgt ggtggtttaa ttaatacagg ggatttaata gaagcactag
    14401 agtcaggaaa aattagagct gctgcccttg atacatttga aactgaagga ttgttcttaa
    14461 acaaaaaaat gaatcctgga gaattaactg acccagaaat aaataaactt ctttctatgg
    14521 aacaagttat attcactcat caccttggtt tcttcactag tacagcgatt gaaaatatag
    14581 tttattctag tttaagtagt gctgtagaag ttataaaaac aggaactgct actaatagag
    14641 taaattagta ttttataaat atattttttc attaattagc tattatctaa caaaatagcc
    14701 tttaatttcc caaatataag agagtcagct cagtatatat tgagtagact ctcttatatt
    14761 attttttata ttaaacttgt actcttctta ttcataccaa attaaattta tattaactta
    14821 ttctaactac tcctactcta tatattttac ctatttttac ttttctaatt atatacattt
    14881 gaatgggtac aatctgtatt ttacaaaaaa aacacctaac aaactatgaa aattgtatga
    14941 ttaatattaa tatatttata agaaaacata acaattatgt tagctctgga tttttaggga
    15001 gggagtatat tggaaaaaat gaataaagtt acttctaaaa aaactaccta tattgttact
    15061 tcagcattat ttgcatccat aatatgcctg acaatagcat atatattaca tattccagtt
    15121 ggtggaaaca atggatatgt tcatatagga gatgctttta tttatctagc tgcaactatt
    15181 ttgccaacaa actatgctat agctgcttct gcaattggtg ctggtctagc tgatttatct
    15241 acaggtgctg ctatctgggt aataccaact ataatcataa aaccaatact tgtattattt
    15301 ttcacttcta aaagtgacaa gataataaat aagagaaata tagttgcttc ggttgtagca
    15361 ggtatagtag gattagtttt atacatgttt gctgaaggta taattattgg aagttttaca
    15421 agtgcatttg taatgagctt acttggccta ttacagccaa taggaagttt tattgttttt
    15481 ataatcttag gtatggcact tgataaactt gattttaaaa aacgttattt taattaagaa
    15541 tttaaattta tggagggaaa attatgaata tcttatatac aattgaaaat agtatctatg
    15601 taaatataac taatacatgt ccatgtagtt gtgttttttg tataagaaac gaaaaagatg
    15661 aagttgcaaa tagtggtagt ttatggttgg agcatgagcc aagtgtagat gaagtaaaag
    15721 aagccttcaa taaatacaat ttagacgact atgatgaaat cgtattttgt ggatatggcg
    15781 aaccattaat gagaataaat gaattgatag atgtagcaaa gtttataaaa gaaaaaagta
    15841 gcataaagat aagaataaat acaaatggtc taagtgattt aatacataac aaaaaaacag
    15901 ctattctgtt aaaagatgtt atagatgcag tatcaataag tcttaatgcc ccaaataaag
    15961 aagcttataa tagagttact caaccaaagt ttggagaaaa atcatttgat tatatgttag
    16021 attttgcaag agattgcaaa aaatatataa aagaagtagc tttttcagta gtagatgaaa
    16081 tatctccaga agaaatagaa gaatctaaac aactggcaaa aaaacttgat ataccattaa
    16141 gagttagaca taagaattag caagtaaaat tataaaacat taagaatcac ctgaatggtc
    16201 tttcaggtga ttcttcctct tgtattaaca ttttacaaaa tagatttcat accacactag
    16261 gaatgtgtaa atcatccaaa tctttctcca attatcagag tttccactaa tgtagtcatt
    16321 aaaaatctca ttaatctcat ctatgttaaa gaatttatct gctatctcac tattgaattt
    16381 atctcggata tccttattat attggtcatc agccatccat atacgaattg gcacaataaa
    16441 tcctaattta tttctaaaag caatctcttc tggtaatgtt tttgatgcag ctgtacgaaa
    16501 ggcaatttta ttctcttttc ctttcacttt atattctgat ggtattctgg atgtaatatc
    16561 aaatattctt gtatctgtaa gaggcattct gacttccaat ccaactgcag tactcatttt
    16621 atctatatta aaataaatat ctccttccaa ccatatttga atatctacat ttgacatgtt
    16681 agtaagtgta tctagttctt ctgtttcttc ataaattcct tttaccactt caataggaag
    16741 cacatcagga ttgtatttct taagaatttt tcttttctca ccctcattca taatgtttgt
    16801 atttccaata taaaatgtct tcaaattctc tccataacac tctgccttat ggtatatatt
    16861 atatccacca aaaaactcat ctgctccttc tccagagtaa cagatatttg tgttttcagc
    16921 tgctgccttg catccaatag caaaaacgat agcagatgca tctccagttg gttgctccat
    16981 atggtacatt acataaggta ctgacgcaaa atactcttca ggtgtgatta tatatctcga
    17041 atttttacac ccaagaatat tagctgtctg ttgtgctaat tttgattcat caaaacgctc
    17101 ttcctcataa ccacatgaac ctgttttttc tatatctgac attgccaata tataagatga
    17161 atcaacacct cctgataaga ataactctgc agtttcattc tctgatttta cctctggcat
    17221 aattttctga agtgttgtat gaatttcatc tgtccattcc tcaatagact tgctgtggtc
    17281 aggatgaaat tcaggtttcc aataacgacc aatacatact gttttattct gatagacaag
    17341 gtaatgtcct ggcataagct tctttactcc acggaagaat gtattttcac ctgctacata
    17401 tgtgaaagac atataaatct gtaacatgtc ttcatttaac tctttcacaa atcctggttg
    17461 ctccataatc ttacgaatag atgaaccata gagaagcttc ccattttcag tctcataata
    17521 atagaaaggt tttactccaa actggtctct taggcagaag agtttctttt ccacagtatc
    17581 ccataatgca aaagcaaaca ttccatgcat gtgatttgcc atatcatatc cccatgtctt
    17641 atatgcttcc actaaaattg ctcgttcacg ctcatatcta cataatgaag aatcgattcc
    17701 aagttcttca caaagaactt tccagtttcg aatatgtcca ttgtattcta gaatttttat
    17761 catatatgtg tcttttcgtg ttaaatactt ttcttctaaa tttatcatat gtgtatcctt
    17821 tcgtattaaa acatttaact tctaaattta ttaaatgttt atatatattt attcacttat
    17881 aatcactgaa aaatcgactt ttttcaaagt aaattctttt tgaattagta ctaatctgct
    17941 ctttattaat ggcataaata tgattatcaa gtattatcag tgttataaca ttg
//
LOCUS       DAFKMX010000079        17727 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFKMX010000079 DAFKMX010000000
VERSION     DAFKMX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.079"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            166..3714
                     /locus_tag="LZB08_002045"
     CDS             166..3714
                     /locus_tag="LZB08_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7315548.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKCSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSKGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3899..5833
                     /locus_tag="LZB08_002046"
     CDS             3899..5833
                     /locus_tag="LZB08_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBM7315549.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5939..6934
                     /locus_tag="LZB08_002047"
     CDS             5939..6934
                     /locus_tag="LZB08_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315550.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMVAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6906..7691
                     /locus_tag="LZB08_002048"
     CDS             6906..7691
                     /locus_tag="LZB08_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBM7315551.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7679..8377
                     /locus_tag="LZB08_002049"
     CDS             7679..8377
                     /locus_tag="LZB08_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315552.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8500..8973
                     /gene="ybaK"
                     /locus_tag="LZB08_002050"
     CDS             8500..8973
                     /gene="ybaK"
                     /locus_tag="LZB08_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBM7315553.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9170..10009
                     /locus_tag="LZB08_002051"
     CDS             9170..10009
                     /locus_tag="LZB08_002051"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBM7315554.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10022..10570
                     /locus_tag="LZB08_002052"
     CDS             10022..10570
                     /locus_tag="LZB08_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBM7315555.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10636..11817
                     /locus_tag="LZB08_002053"
     CDS             10636..11817
                     /locus_tag="LZB08_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBM7315556.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12112..12498
                     /locus_tag="LZB08_002054"
     CDS             12112..12498
                     /locus_tag="LZB08_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7315557.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12648..12911
                     /locus_tag="LZB08_002055"
     CDS             12648..12911
                     /locus_tag="LZB08_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBM7315558.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13040..13882)
                     /locus_tag="LZB08_002056"
     CDS             complement(13040..13882)
                     /locus_tag="LZB08_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBM7315559.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14177..14911
                     /locus_tag="LZB08_002057"
     CDS             14177..14911
                     /locus_tag="LZB08_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7315560.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14921..16054
                     /gene="nagA"
                     /locus_tag="LZB08_002058"
     CDS             14921..16054
                     /gene="nagA"
                     /locus_tag="LZB08_002058"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBM7315561.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            16153..16902
                     /gene="nagB"
                     /locus_tag="LZB08_002059"
     CDS             16153..16902
                     /gene="nagB"
                     /locus_tag="LZB08_002059"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBM7315562.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            complement(17034..17453)
                     /locus_tag="LZB08_002060"
     CDS             complement(17034..17453)
                     /locus_tag="LZB08_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315563.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFYTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKKDGSWYIDIPRLLLLCFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
BASE COUNT         7096 a         1866 c         3121 g         5644 t
ORIGIN      
        1 tgaataaaga ttttacaaat aaatcttaag ctatcttatt taattctaat acaggaagaa
       61 ggtaaatgta tattgaaaaa atcatagaaa tagaagtaaa caatttaaat aaatacaaag
      121 gtttgcaaaa gaagtaatat aattatatac aatgaggatg tgagtatgat gaacagacta
      181 aagccaagaa ttttaggtat tagtatcttg tttattatta taattatttt aacatgtgga
      241 gctgttttca agtttaaatt taaattagat aataaattaa aagaaaccac acatacatat
      301 ttgtctgagt ttacagaaca caatataatt aatctcaaga caagacttag tggtcaattt
      361 gacatgcttg aatctattgc taacttcata ggaaacttga ataatataaa tgatgaaatg
      421 gtgattgatt taatggattc tgatgtaaag tgcagttctt taattagaat gactatctct
      481 acattagatg gggaatgtta ttctaatgat ggaattaccc ataatgttca agatagagat
      541 tattttaaaa aagctaaaaa tggagaaaag aatatatcag aacctttaaa atctattgtt
      601 aataataaag aaataatagt tttatctacc ccagtttata aaagtgggaa aatagttggt
      661 gttttaagtg gaatttatga ttcaaaaaag ttaaatgagt tattaggatt atctgcattt
      721 gatgaaaaag cagatgtttt tgttgcaaag aaaaatggag atatagttgc tcaaaaaaca
      781 aataaaaata gaaagacgct atcatgtata tttcagcttt ttgacaaaga taattgtgat
      841 gaagatgatt gtctaaaaac tataaaagaa aatatgaaaa atgataagtc tggatatttt
      901 aattataact tagataatga aaaatttatg ttaaactatc agccacttgg agttaatgat
      961 tggtatgttt tttctgttgt gccaaataaa atagtatcta ttcagtcaga gaatatatct
     1021 tatgatgtat atatttttgc attagaaata ctactagtta tattgatttt tataatatat
     1081 atagcatgta ttataaataa taacaccaaa attattataa atgaaaaaga aaaatttaaa
     1141 gtactcactg ataatatatt aggaggggtt aaaacctgtt taaaagatga atatatgact
     1201 atgacatatg taagtcaggg gtttttagag cttacaggtt actcttgtga agatttaaat
     1261 aaaatattta aaaatagatt taaagacatg atttataaag atgatttaga caagtttaat
     1321 tgtattattc taaatcaatt agaaaaacaa gataaaattg aagttgaata tagaattgta
     1381 agaaaagatg gaagtataat ttggattttg gagagaagta agtctataaa tgataataaa
     1441 ggaagtgagt gtattcaggg agttttaact gatataacag aacttaagaa aatacaagaa
     1501 gaattagatt caaaaagatt tgaaatacag gctatcaatg atagtattgt tggtggaata
     1561 gtgataactg agataaatga tgattttgat agtatatatt taaatgaagg ttatttaaat
     1621 atgattggat atacacgcaa acaactggaa gatgagctaa ataataaatt aatcaaactt
     1681 atttttaaag atgatagaac agaattaatt aatagtataa acaaacaact caaattaagt
     1741 gatacatttg catctgaaca aagagttgaa aaaagggatg gcactattat atggatttta
     1801 ttaaaaggaa gaaagatgat ttatgataat ggaaaatctg tactttgttg tgttattgta
     1861 gatattactt ctgctaaatt attagagcaa gaacttaaaa agagtgaaga acgtcatgaa
     1921 atcattctaa agcagacgca aaatattata atagaatggg atatagaaaa agataaaatg
     1981 aatatatcac ctaattggta taataagttt aaatatgacc ctataactga aaacgcaaca
     2041 aaaaatataa ttaaatcagt agatatatat catgatgata tggataagtt tatttcatta
     2101 attaaactta cttcaaaagg tagtgaatat gaagagtgtg aaattagact tagaactaga
     2161 gatactattt acgcttggtt tagtataaag gcaacaactt tatttgataa taatggtaaa
     2221 ccttataaag tagtaggaag tttgactgat attgatgaat caaaacgaga agctgaaaag
     2281 ttaaaagaaa gggcagaaaa agaccctctt acagatttgt ataataaaaa agcatcacaa
     2341 tcacttatag aagagtacat aacattggat aaaaaaagaa aaggtgcttt aatgattatt
     2401 gatattgatg actttaaagg aataaatgat aatttaggac atttatatgg ggatgctgta
     2461 ttaagtgaaa ttgcaaatga cctaataaat ctatttagag caaccgatat agtaggaaga
     2521 attggtggag atgagtttat tgtatttata aaagatattt ctgaaacaag agatattata
     2581 aataaagcag aagaattggt aaaaacattt gaaaggtctt ttttaggtaa aggacgaaat
     2641 tataaaatat cattaagtgt aggaattgct aagtttccac aagatggtat ggactatatg
     2701 acactattta gaaatgcgga tatagctctt tactcagcca aagggaaggg taagaattgt
     2761 tatacattgt atgataaaaa aattgataaa gtccaatata taactaatac aagaaatgca
     2821 ggagatagtg ataatgtaga gatgtctttt aaagaacata tcacaaatta tatatttgat
     2881 atactatatg aatcaagtga tatagaagtg gcagtaaatt taattctaag tattgtaggt
     2941 aaacacttta atgtgagtcg catgtatata tttgaaaatt cagatgataa tcgagtatgt
     3001 aaaaatacat ttgaatggtg taacgaaggt gtaacctcgg aaattaataa tcttaaaaat
     3061 atttgttaca aggatttagg caactatctt ttaaatttta atgcagatgg gttattttat
     3121 tgtaatgata ttgaaaattt aggtagtgat gtgaaaaaag tacttaagcc acaaggcata
     3181 cgttcaatat tacaatgtaa aattttagag ggtaatgaat ttagagggtt cataggattt
     3241 gatgaatgtt catataatcg tatgtggact aatgatgaag tgaacacttt gacatttata
     3301 tctaaaacgc ttagtatgtt tttattaaaa atgcgtattc agagtaagct agaagagtct
     3361 ttaacaattc aaaaatctgt gatggataat atggatatat gggcatatgt tgttgacagg
     3421 aatacctatg aattactatt tatcaataaa aaaatgcttg agtcagcacc aaacactcat
     3481 gtaggtgatt tatgttataa agcgatatgg aataatcaag ataaaccttg taaaaaatgc
     3541 cctatgatat atttaaaaga tggtgatgat acttataaat cattcatgca taataaaaag
     3601 tttgaggtac aggcaaatat aactacaact actttgaaat ggtttgatgg taaagatgca
     3661 tgtttaatat ttggaagtga aataactagt attgaagctg aagaaaatac ttaaaatact
     3721 gtgttatata tatacttaaa atcgcaattt gaatttatta gacttaaaat gtctatataa
     3781 atcatttaag ttgtggtttt aagtattttt tatgatataa ttaagcgtta agaacaaaat
     3841 taaaagatac ttttgtataa aaaaatattt gttaaatata tattgagcag gtgataatat
     3901 ggaaaataag tttttaccaa taagcaaaca agatatgata gatagaggat gggaagaatt
     3961 agattttgtt ctagttacag gagatgctta tgtggaccat catagttttg gaacagcaat
     4021 aatatctaga gttttagaaa gtgctgggta taaagttgga ataatagctc aaccagactg
     4081 gagaactaca gatgatttta tgaagctagg aaaacctaga cttgcatttc tagttaatgc
     4141 aggaaatatg gattcaatgg tcaatcatta ttctgtaagt aaaaagcata gagaaaagga
     4201 tatgtattct ccaggtggga aaatgggtta tagaccagat agagctacta tagtatattg
     4261 caataaaata agagaagcat acagtgatgt tgcaatagta ataggtggaa ttgaagcgag
     4321 cttaagaaga tttgcacact atgattattg gagtgataag gttagaaagt ctatgttaat
     4381 agatagtgga gcagatttat tagtttatgg tatgagtgaa aagcagattg tagaagtagc
     4441 caatgcactt aatgatggat atgacccaaa atacattaga catattgatg ggacatgcta
     4501 tatttcagat acattggaag aaatatatga taaatatata ttaattccat catataaaga
     4561 aatttgtgaa gataaaatga agtatgtaga agcttttaaa attcaatatg atgagcaaga
     4621 cccatttaga ggtaatataa ttgttcaacc tcatggaagt aagtatctgg ttcaaaataa
     4681 gccagaaaaa ccacttagta gagaagaact agatgaagta tatggattac catatcagaa
     4741 gacataccat cctgtgtatg aaaaatttgg aggaatacca gctatagaag aagtaaaatt
     4801 tagtatagta agttcaagag gctgttttgg tagttgttca ttttgtgcaa taacatttca
     4861 tcaaggtaga gcagtgcaaa gtagaagtga aaaatcaatt attgatgaag caataggaat
     4921 aacaaattta gatgatttta aagggtatat acatgatgtt ggaggtccaa cagctaattt
     4981 tagaagacca gcatgtaaaa aacaaattac taaaggtgct tgtaaaaata gacaatgctt
     5041 atctccatca ccatgtaaaa atttagatgc agaccacagt gaatacctac atttacttag
     5101 agctgtaaga aaattaccta aaataaaaaa ggtgtttgtg cgttctggaa ttagatacga
     5161 ttatgtaatg gcagataaga acaataaatt tcttagagag ttgatagagc atcatgtaag
     5221 tggtcagtta aaggtagccc cagaacatat atcagaagaa gttttaaagt atatgcaaaa
     5281 accagcagga aaaacttatg ataaatttag acagaagttt tttgctataa atgaagaatt
     5341 aggtaaaaag caatatttga taccctattt aatgtctagt caccctggtt ctaccttaaa
     5401 tagcgctata gagttagcag agtatttaag agatactcac taccaaccag agcaggttca
     5461 ggatttttat ccaactccag ggactctatc tacaaccatg ttttatacag gtattgaccc
     5521 gcttacaatg aagccagttt atgttcctaa atcaaaacgt gacaaggcaa tgcaaagggc
     5581 tttattacaa tatagagcac ctagaaatta tgatttggta tactctgcgt tagttgaagc
     5641 aggaagagaa gacttaattg gttttggaca tagatgtctt ataaagccaa aaaatgaaaa
     5701 gccttatttt aatagaaata attctaaaaa aaatgtatct aaaggaacaa ataaaaacaa
     5761 aaaaactaat acaaataaca gaagtaataa aaatcaacaa aaaagttcaa ctaaaaaaaa
     5821 gaaaaaacat taaagtataa ataaaaatac ttaaaaaaga aggattattc gataaattaa
     5881 cagaaattag tataaataaa tataattatt ttaatgacgt tacctttggg aggaagagat
     5941 gaaaaagtat atttctataa tattattagt ggtgttgact atggttgttt tggtaggatg
     6001 ttcaccagga aaagataacc ctaaaaataa ggagctatca gttgtcaaac aagtaaaagt
     6061 agctgttcca gatggactac cagcagtggc aatagctaaa cttgcaaatg aaaatccaga
     6121 gattaaagaa ggatatgaaa cagtgtatag cattgaaaag acacctgagg cgatttccac
     6181 tagagtgatg aaaaaagatg cagatatagc tatagttcct tcaaacatgg ttgcaatagc
     6241 gtataataaa acttcatcat ataatattgt tggaactgta ggaatgggtt cactttattt
     6301 ggtgtcaaca gaaaatataa aagattactc tgatttagtt ggtaaagaag ttggatgtac
     6361 tggaaaagga cttactccag acataactat aaaaagtctt ttgttacaaa aggatataaa
     6421 ttattcagat ataaaattta attatgtaaa ttcagcaagt gaactggtac cattattggc
     6481 tactggtaaa gttaaaacag gtattgttcc agaacctgca cttagtgctt tgatgtctaa
     6541 aaatccagac ataaaaatta taaaaagttt aaatgactca tggaaggaag taagtggttc
     6601 aaaagacggt tatcctcaat ctactttaat tgtaaagtct gaatttttaa gagataataa
     6661 agattttgtt gattcttttg taggtcaatt atcaaatagt atagattggg caaacaaaaa
     6721 tcctgaagaa ttaggtgcat attctgaaaa aataaaaata tctactgaat caaagataat
     6781 aggaaaatct cttgaaagag ctaatttgaa atatattcca gttaaggaca tgattaaaga
     6841 ttataaaaat tactatgaga aattagctaa ttttgatgat aagacattag gaggcaaagt
     6901 accagatgaa gcaatatact ttgttgagaa ataaaaaaaa taaagaacaa tttatagata
     6961 aaatggaggt aacactttct tgtgttatcc tcttattttt atggcaaatt attgctttaa
     7021 aaattgataa tgatattttt ttaccaacag taggtcaagt atttaatagt ataaaagaga
     7081 ttatgctaaa ttctagtttc tatatagata tactttactc tatggggaga tgttttttta
     7141 gttttttact ggctatgatt tttgctatta tactttcaat agtgtcttat ttaaatagat
     7201 tttttagaaa cttattaaag ccaatcaatg ctttgacacg ttcaatacca actatgatat
     7261 tggtagtact tgcacttatt tggtttgaaa aggatagtac cccttttata gttggattta
     7321 ctatagtttt tcctatattg tatgataacg ttttgggcgc tatattgaat atagacaaga
     7381 acttattaga aatggctaat gtgtataaaa taaggtttat agataaggtg ttaaaaattt
     7441 atttaccagc cataaaattt caaattatat ctatactagt atcaacattc tctttaagtt
     7501 tgaaagttgc tatagcagga gaggtatata gtcaacctac atatggtatt ggaactatga
     7561 tacagactga aaaaattaac tttaacacat ctggaatatt tgcatggata attattgttc
     7621 ttttaatatc tgctatttta caaatagctc aaaagttttt agcaaggaga gcttttttat
     7681 ggaaaagata aagttgagta ttgaaaatat aaataaaaga tatgatagtc gtattatatt
     7741 tagagatttt aatattgatt tttatgtaaa tgaggtaaat tgtatccttg gaaaatctgg
     7801 ttgtggaaaa actacactct tgaacataat aagtgggatt attaaaaatg acaccaacaa
     7861 tctaaatatc aaagaaaatt taaatagagt aggaaataag cttgaagcta gttacatatt
     7921 tcaagatgat agacttatag actggttaac agtagaagaa aatataaaga tagttgtaaa
     7981 taaatactat aataaaactc aattaaataa aatttgtgat gaatacttgg aattggttgg
     8041 aattagtgat tataaaaaat tctatcctca gatgcttagt ggaggtatta gacaaagagt
     8101 aaatattgct agagctttta tatatccatc aaaaaatatt ataatggatg aaccttttaa
     8161 atctattgat gcaaagaata cacagcttat aatggataat ttcagaaata tattaagaaa
     8221 agaaaaaaga actgtgttat ttgtaactca taatattgag gaagcattgt ttttagcaga
     8281 taggatattt attttaggag actctccaat tagaataaaa aaaatattaa aaaattctaa
     8341 ggaattggaa aaaaatgaag ttttgaagtt gatatagagt tataataata ctgtcgtttt
     8401 aaaatatatt tggttaaaat ctataagact atattaaaat agtacagaat attcaaaact
     8461 gtgtataatg ttcgtatttt ataaaaagga agtgaatgta tggttaagac taatgctatg
     8521 agaatattag attcaaataa gattgattat aaagtaatgt cttatgaagt gaaaagtgaa
     8581 catgtggatg gtgttgaggt ggcacatgat ataggaagag atgtaaatga agtgtataag
     8641 acattagtaa ctcaaggagt tagtaagaat atatatgtat atgtaatacc agtacatgaa
     8701 aacttagact taaaaaaggc tgcaaaagta gctaaagaga aaagtgtaga gatgattcat
     8761 gtaaaagata taaataagct tacaggttat attagaggcg gatgttctcc tataggtatg
     8821 aaaaagcttt ataaaacttt tgtaaatgaa agtgcaaaga atttagatac aattattgtg
     8881 agtgcaggaa aaataggata tcaaatagaa ctatctccat ttgatttaca aagacttata
     8941 aaagtagaat ttgtagatgt aataaaaaaa taagaattac atataaaata tagagcttat
     9001 attctcaata ataggtgttg tagtattaat aaaatgaata ataattttaa aataaatatt
     9061 cagttactta tgtgtaattg gtataaaaat attgagaaat tttaaaaaca atggtaaagt
     9121 gtaagtataa tcacgaatat tttttataaa gtaatggagg gatgttcgct tgagaaaaat
     9181 aaaatctgaa caaatcgtcg agcaggtaaa aaaactttgt atagaagcga gcctatattt
     9241 aggagaagat gttttaagct gtataaaaga aaaagcgaaa agtgaaaaga gtgaagttgg
     9301 aaaaaatata ttgaatatat tagttgaaaa tgctgaaata gctaaagaaa aaaatatacc
     9361 aatatgccaa gatactggta tggctgtatt ttttgttgaa ataggacaag aagttttaat
     9421 tgaaggagac acacttactg atgctataaa tgagggagta agacaagggt atgaagaagg
     9481 ctacttaaga aaatctgttg taagtccaat taacagagta aatacaaaag ataatacacc
     9541 tgctgttatt cattatgata tggtaaaagg tgataaaatt aaaatagaat ttgcagctaa
     9601 gggatttgga agcgaaaata tgagtaagat gaaaatgtta aagccatcag atggattgga
     9661 aggaattaaa aaattcataa tagatacagt ttcagaagct ggacctaacc catgccctcc
     9721 tatggtaatt ggagttggta taggtgggac tgtggacaaa tgtgctcaaa tagctaaaaa
     9781 ggcacttttt agagaactag gtgaatttaa taaggacgaa aatatagcaa aacttgaaag
     9841 tgaactatta acagctatta ataaacttgg tataggacct caaggcttag gtggtactac
     9901 aacagctttg ggattaaaca tagagacttt tcctacacac atagcaggtt tacctgttgt
     9961 tgtaaatata aactgccatg catcaagaca taagaaggta gtaatttagg aggaaaataa
    10021 aatgataaaa ataactacac ctgtaaatga aattgatata gcaaaattaa attgtggaga
    10081 tacaattagt ttgagtggta tactttatac agcaagagat gctgctcata aaagattgat
    10141 agattgtata aataaaggtg aagaattacc atttgacgta tatggtcaag gaatatatta
    10201 tgtaggacct actcctacaa agccaggaga agttataggt gctgcaggtc caacgacgag
    10261 ttatagaatg gatgatttga ctatacctct tttagaaaga ggtcttagat tgatgatagg
    10321 caagggaaaa agaagtgatg aagttataga aggtatgcaa aaatacggag cagtttatct
    10381 tgctgcaata ggaggagctg gagcgtatat ttcaaattca ataaaatcat gtgaaattat
    10441 tgcatatgaa gatttaggag ctgaagctat aagaaagatt gtagtagaag accttaagct
    10501 tacagtagca atagatagct atggaaataa tatatatgag caaggtcgag caatttatga
    10561 atgtaaataa tgtttagaaa taaatatgaa tatcaaaatt aaaaatagtt gtgtatattt
    10621 taaggaggaa aattgatgtc taaggattat gcaaaattgg cattagaaat gcatgaaatc
    10681 aacaaaggga aagtaagtgt agaaagcaag gtagaaatta aaactaagga tgatttatca
    10741 acagcatata caccaggggt tgctgaacct tgtttaaaaa tacatgaaaa tcaagatgat
    10801 gtttatagat acacttctaa agggaatcta gtagcagtag ttagtgatgg aagtgctgta
    10861 cttggattag gtaacattgg tgctgaagca tctatacctg ttatggaagg taaagctata
    10921 ttatttaaac aatttgcaga tgtagacgct tttccaatat gtttaaagac taatgatgta
    10981 gatgagatag ttaaatctgt agagttgatg gagcctgtat ttggaggtat aaatttggaa
    11041 gacatatctt ctccaagatg ttttgagata gaagagagat taaaaaaatc tttaagtata
    11101 cctgtttttc atgatgacca acatgggaca gcaataatag ttgcagcagc cataataaac
    11161 tcaataaagt taattgaaaa taaaaaaata gaagatttag aaatagttat aaacggagca
    11221 ggagctgctg gtatagccat agcaaagata ctacttaata tgaatgttaa aaatataatt
    11281 ttatgtgata gaagtggagc attggaagct agtatagaaa atctaaacta tgttcaaaaa
    11341 gaaatgttaa aagtaacaaa tataagaaat gaaaaaggac ctttaaaaga tattataaaa
    11401 ggaaaagatg tatttattgg tgtttcaggt ccaggagctg ttacaaagga aatggttgaa
    11461 agtatgtctg agaagccaat aatattggca atggcaaatc ctactccaga gataatgcca
    11521 gaagaagcaa aattaggtgg agcatttata atgggaacag gtcgttcaga cttccctaat
    11581 caagtcaata atgtactggc ttttcctgga atatttagag gtgcacttga tgttaaggct
    11641 aaagagataa atgaagaaat gaagatagct gctgcatatg caattgctaa tacaataagt
    11701 gatgaagaaa tatgcccaga gtatatatta ccagatgtat ttaataagaa tgtagtaaaa
    11761 aatgttgcta aggcagttaa agaagcagcg ataaaaacta aagtaaatag aatataaatt
    11821 tttatatgtt catatatcct tatattatcg ggtatatggg catttttatt ataggtttat
    11881 actcatatat ccttactttt atatacatat ctttatgatt aaaggtgtta gaaaatgtag
    11941 taaattttat actatcagac tctatcttta ttactttagt agatacatgg ttgtaaaggt
    12001 ttatagaaaa aaatatagga aggcagattt ttattgttgc gtatatatga gaatgatata
    12061 atatgaatat tataaattaa taaatttaaa tttatggagg tacaagagat gatggaatat
    12121 aaagttaatg accaagaact taatgcttca atgtttatcc cattcgtcaa caggatatgg
    12181 ccaggagatt atgatatgga taaaacacag actgcattat caaaaacttt gaatataact
    12241 gcttatgatg ataaagtgct tgtaggatgt ctgcgagttc tttcagatgg ttattatttt
    12301 ggaacaatta cagaattgct tgttcttcca gagtatcaga aacaaggcat tggtagtaag
    12361 cttttccagc ttgctaaaga gaacacccca actatgctat attttggtgc acagccagga
    12421 gtagaagagt tctatgaaaa aaatgggtgc caaagaagtc tgcaatccta cataataaaa
    12481 aagaaagata gttattaaag ttccagcttt caataatctt aaaagtacaa tattttagtg
    12541 gaatcatttc attttttatt actaatatga cataaacatg tcatattagt attgatatac
    12601 ttttaatatg gttaagagcc aaagaaaaat ataaggagtg atttaaaatg gaagaaacta
    12661 aattttgcca atgctgtgca atgccaatgg gacaaactga tgaattatat gggacaaata
    12721 aagatggaag taaaagtact gattattgta gttattgttt taaagatggt gagtttacag
    12781 ctgatataag tatggaagag atgatagaag tttgtatccc tcatatgctt caaagtaata
    12841 aggatatgac tgaagatgaa gcaagaacaa tgatgaacaa tttctttcca acattaaaac
    12901 gctggaaata attttatgca aaattagtta tatgcaaaac atgaaatatg gtgttgagaa
    12961 taaggggtgg tgtgctttat agcataccac ctgttttgtt acttggagtt cagttatttt
    13021 cctaaatatt ttaaatatac tattttagat aagttccctc ttcatcttga taaattaatc
    13081 cttttttcat aagcactcca agagctcgtt taaaattttt cttacttgaa ttaaataata
    13141 tagaaatatc tttagggtca gatttatcat taaatctcat atatccatca gaaccttcta
    13201 aataacttaa aatcttattt tctaaagtat caagttcatc ctttctattt cctctaggag
    13261 aaagaccaag ttttccatct tcatatatct ttatgacatg taaattttca agaacatcac
    13321 cagtttttag ttcagtaaag tactcattat gaagtattac accagcatat ttattgtcta
    13381 cacatatcat tgcagaatta ttagtttgga atccatatac aactccagtt acaatatctc
    13441 ctacattgta agtatggtct gttaataagt atgaatctat atctgtagta gcagcaattc
    13501 ttttgctttt gtctaaatat atataaaata aatatttttc gtctctaaat agtggataag
    13561 tcttagcttt aaaaggaact aatatgtctt taggaagtcc catatctata aaagtaccaa
    13621 tgtctgtatt atcaactact tttaaatgtg ctacatcccc aaccttagct aaaggcggta
    13681 ttaaagttgc agcagttctg tcatcagaat ctttaaatat gaaagcattt acttcatcac
    13741 ctatgtttat ttcatttttt ccgattaatc tattatgaag aagtatatcg tcttttgtgt
    13801 tattagtttg tccatccaaa aaatatccaa attcagtttt tctttttact gtaagtttat
    13861 taaaatcacc tatgtttatc aaattaaaat catcctctca ttttatattt ataatttagc
    13921 cataatatac ttatgtagta gtttgtcaca gtatctatat tagtattagt gaaatttaca
    13981 ataaataata tagttattta tgtatttttc aattctctag cttcttatat agtataaact
    14041 ccaatttctt ttttgtcttt aggttttatt aaaattttta caaaataagt taaaaagtat
    14101 tgaaataaaa tttcttttaa agtataattg gtatatacca aaattggtat ataccatatc
    14161 gaaaagtggt gactatatga aaatagtaaa taaatctagt aatataccat tgcatactca
    14221 attaagttca ataataagag agatgataga aactggtgag cttaaagagg gcgatgcaat
    14281 aatgccagaa agagaacttt gtaacataca aaatgtaagt cgtatgacag taaataaaac
    14341 cattgttgga ttagttacag aaggacttct ttatagggta caaggtaaag ggacattcgt
    14401 tgcaaagcaa aaaaagaagt atcagttttc aaatgtaaaa ggttttactg atgttatgaa
    14461 agaaaaggga gtaaacataa aaacggatat attatccttt gagatggaac taccagatga
    14521 tttagttaaa agaaagcttg gaataagtga taatactaca aatatatata agattgtcag
    14581 acttagatat actgatggag agccatttgg attagaaata gtatatctat cagaagaaat
    14641 gtgtaagggt ctaacaaaag ggatattgga taattcttca ttatataggg ttttaaatga
    14701 aaaatatggc tataagatac aaaaagctga acaggtaatg gagccagtaa tactttcaga
    14761 tgaagaaagt aagcttcttg aaacagatga aggtgcttta gctcttaaac ttcatagaaa
    14821 ttcctataat agagaaggta gccctataga atataccata tcaatattta gaacagataa
    14881 atatcaatat gaaatagtat tgagtgagta ggtgaataat atgaaatgtt taattaatgg
    14941 aaagataata ttaaaaaatc aaatcttaga aaataaagta ttagtatttg atgaaaaaat
    15001 aattgatata gctgatagtg taccaaaaga ttgtgaagtt atagatgctg atggaaaata
    15061 tatatctcca gggcttattg atattcatat acatggaaac atgggaaaag atacaatgga
    15121 ttcgactgat gaatctattg aaacaatttc aaagtcaatt atgagacatg gagtaacatc
    15181 ttttcttcca actacaatga ctatggataa agaacatgtt tatgatgcac ttgaagttat
    15241 taaaaaagcc caaaatagaa agcttgaagg ggcacaagta ttaggagcac atttagaagg
    15301 accttttata aatgagaatt ataagggagc acaaaatgaa aaatttataa taaattcaaa
    15361 atacgaattt ataaaagaat ataaagatgt tataaaagtt ataacttatg ctccagaaaa
    15421 agatattgat tttgatttta ctagagaaat aaaaagatgt acagatatag ttttatctat
    15481 tggtcatagt aatgcaaatt atgaccaagc taaagaagct ataaatttag gagtaactaa
    15541 tgtaactcac atgttcaatg caatgactgg tttaaaccat agagacccag gcgttgttgg
    15601 tgcagcactt acaactaatg tgtacagtga gttaatagca gatacaatcc atataaataa
    15661 agatttattt caatttatac taaataataa aggaaaagaa agactgattt taataacaga
    15721 ttctatagaa gctggtggtc ttgaagatgg caattattct cttggaggtc agaaagtcat
    15781 agttaagggt aatgaagcta gacttgaaaa tggagcatta gctggaagtg tgttgtcttt
    15841 aaataaaatg gtatttaact tcttagataa tacaaacctt aaagtgaatg aagcaattaa
    15901 tttagcttct ttaaatccag caacatcttt gggaataaat gacaaaaaag gaagcttaga
    15961 aataggtaag gatgcagata tagctgtatt tgatgagaat ttagattgta aaatgacact
    16021 ttgcttgggg gaagtagtat ataaaaatat atagtataca aaacaaactt taaagacagt
    16081 aaaaacaata taaaaaataa taaattaaac ttacattagt taaagtagta gttaaaattt
    16141 aggaggaata agatgagagt attagtgtgt aaagattatg atggaatgag taaaaaagcc
    16201 gctgaaatga tagcagctca aatagtttta aaaccaaatt caatactggg actagctaca
    16261 ggaagtacac cagtaggtat gtatagagat ttagtaaaaa aatacaatga taatatagta
    16321 gatttttcag atgtgatgag ctttaattta gatgaatatt ataagttacc aatatctaat
    16381 gaccaaagct atgattattt tatgaaagag aatctattta atcatgtaaa cataaagcca
    16441 gaaaatactc atctaccaaa tggaatggct gatgatattg aaaaagagtg tatgaactat
    16501 gaagcttcta tagatgcagc tggtggtata gatgtacaag tacttggaat aggtcgtaat
    16561 gctcatatag gttttaatga acctgatact aaatttgcaa agagaactca tgtagttgaa
    16621 cttacagagt ctacaataga agctaatgca agattcttta aatcaagaga agatgttcct
    16681 aaaaaagctg taagtatggg aataggttca atattgaagt ctaagaaaat attgctttta
    16741 gcttcaggag aagaaaaagc tgatgcagta tataatactg tttatggaga tataactcca
    16801 gaagttcctg gttctatact tcaacttcat aaagacacta tagttatagt agatgaagca
    16861 gcagcaagta aattgaatcc aaaagattat aaattagtat aagaatagaa tgtagtttaa
    16921 gtaaaaaata gctatcattg aataatcaaa tttatttaat gatagctatt tttatttaaa
    16981 ttttttactt ctttttattt aaattagtta ttattgttta aattacttgc ttattacttt
    17041 ttacgaaaac tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt
    17101 gggattctat caagcataat aaaattacct ataattggga tattaccaac tggaaacata
    17161 aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt
    17221 ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct
    17281 agtaataaac caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct
    17341 tgcatataac cagtgtaaaa ttgagtatag aaatcataat ttaatttaac agctaaaaaa
    17401 tacataagca atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat
    17461 tctcccttca gaaatacttt attcagtttt attttgttgg aatctgattt tatcatttag
    17521 tttaagaaat ccataatatt agtgaagaat gaaataagta ttatatatga ataaatattt
    17581 ttagaattaa ggtgaattgt agtgcttaat ataactataa tattaattgt ttgaatattc
    17641 aatgaaaagt ttgaaaaata tcaattttga tataaactga ataaatacag ttgaatatta
    17701 aattattatt ttatcaaaaa tatgcaa
//
LOCUS       DAFKMX010000080        17590 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFKMX010000080 DAFKMX010000000
VERSION     DAFKMX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.080"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(269..1423)
                     /locus_tag="LZB08_002061"
     CDS             complement(269..1423)
                     /locus_tag="LZB08_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315564.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(1426..1893)
                     /locus_tag="LZB08_002062"
     CDS             complement(1426..1893)
                     /locus_tag="LZB08_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7315565.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1907..2395)
                     /locus_tag="LZB08_002063"
     CDS             complement(1907..2395)
                     /locus_tag="LZB08_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315566.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSSF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIRQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(2409..3740)
                     /locus_tag="LZB08_002064"
     CDS             complement(2409..3740)
                     /locus_tag="LZB08_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBM7315567.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGSIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(3754..5319)
                     /locus_tag="LZB08_002065"
     CDS             complement(3754..5319)
                     /locus_tag="LZB08_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7315568.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(5489..6274)
                     /locus_tag="LZB08_002066"
     CDS             complement(5489..6274)
                     /locus_tag="LZB08_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7315569.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(6441..7640)
                     /locus_tag="LZB08_002067"
     CDS             complement(6441..7640)
                     /locus_tag="LZB08_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBM7315570.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(8162..9499)
                     /gene="xylA"
                     /locus_tag="LZB08_002068"
     CDS             complement(8162..9499)
                     /gene="xylA"
                     /locus_tag="LZB08_002068"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBM7315571.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(9595..11145)
                     /gene="xylB"
                     /locus_tag="LZB08_002069"
     CDS             complement(9595..11145)
                     /gene="xylB"
                     /locus_tag="LZB08_002069"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBM7315572.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKIND"
     gene            11353..12513
                     /locus_tag="LZB08_002070"
     CDS             11353..12513
                     /locus_tag="LZB08_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBM7315573.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(12669..13073)
                     /locus_tag="LZB08_002071"
     CDS             complement(12669..13073)
                     /locus_tag="LZB08_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315574.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(13094..13576)
                     /locus_tag="LZB08_002072"
     CDS             complement(13094..13576)
                     /locus_tag="LZB08_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315575.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(13596..14405)
                     /locus_tag="LZB08_002073"
     CDS             complement(13596..14405)
                     /locus_tag="LZB08_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBM7315576.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(14398..15177)
                     /locus_tag="LZB08_002074"
     CDS             complement(14398..15177)
                     /locus_tag="LZB08_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315577.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(15177..17255)
                     /locus_tag="LZB08_002075"
     CDS             complement(15177..17255)
                     /locus_tag="LZB08_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBM7315578.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         5884 a         3144 c         1927 g         6635 t
ORIGIN      
        1 ggttttatat aaataaatcc ttaagtaaat attacatttt aactgttagc attacacttt
       61 tgtaacatat ggaagactat tataattata atggaaatat cttaaaataa aagcatgatt
      121 tacttctttg tataaacaaa gaaaagaagc tcctaagaag cctctaatct atacaattat
      181 tcttctctat aaaaaaccaa aatacacatt gattgtggca aatacagata ctataaacaa
      241 gtgatattac tactatatat taaaacaatt attcctgaag ataattcaat aagactatca
      301 agcaataagc taagcatcca aaaagactta ataccattaa tgtataatta tacccaatat
      361 taatgctcaa aaaaccagca acagttggaa taatcagaga tgaacctccc ataaatatac
      421 ttaaagctat taattggcta ctacttctaa gttttttagg agaaacttca aatattaatc
      481 tttttgaagt aattaaaatt aaaggagtag aaaacacttg aaaaacacac agtataataa
      541 tgatatttga atcatttgtt aaagcaaata atagaaactg aactgttatc ataatagcac
      601 ttaattttaa taagtttaaa ctgctatatt tttttaataa ataagaacca gccagatatg
      661 ttggtatctc taacaatgac tgtaaagacc actttaaaga tatctgagaa tttgtagcat
      721 ttaaagctat cattttatct attacagtac agttatttgc taccaccatt gagtataata
      781 agaataaaat acatactagt aagatatatt ttttatcttt taataattca ataatatctt
      841 tattagttac atttattttc ttattaatct tttcaatatc aggtaataat agtatattta
      901 taataagtaa tactacaatt aaaccaatcg ctacagataa tccaaaatat gagaatctaa
      961 gaatcaagta agatgctacc atacttccaa tagcccatcc aatcgaccca aaagtcttaa
     1021 taaatgacaa ataattatta atattatctc cacaacctat tatccatgta tcatataaac
     1081 tacaacaagt atttaataat cctcctgata aagcaacaag taataagtat attataaaag
     1141 ttgcagattc agtagaaaac ataataactg atgcaattcc ataaactgat aaactaatta
     1201 aaacaatttt tttcattgtt tgatacttat cagctaatac tccaaataat aattgaaaca
     1261 atatattggt tattgcatag ctcgataata taattcctct ttcaaacgct gagtaaccta
     1321 attttgataa gaaaggtgtg aattttgttg aagcaaaggc aaaaataata tatgagaaaa
     1381 aatagaatat gataaattta aatttaatcg ctttattaat cataatcaat caaaattgat
     1441 aactttaata ccactatttc cttcctgatt taagtttgaa agatgatatt ttaccgtatt
     1501 aggattctct acaataacta aaggaatata taaagtattt tcaagtaact tattatcaat
     1561 ttctactaac ttttccccca tattaacttc ttgaccttgt ttaacttcta atttaaagtt
     1621 ttcttttttt gttgttgcac tatccaatcc aatatgaata agaatttgaa catcattttg
     1681 caatttaatt cctatagcat ggttagtttg agcaataaaa cttattactc ctttagtcgg
     1741 tgatttaaga atattgtcct ttgctatcat agcaaatcca tctcccatta ccttagttga
     1801 aaatacttta tcaggaattt ctccaagatt aattattttt ccatcaatgt atgaattaat
     1861 tgtaattgct tctttttttc ttttaaagaa cattatagac actcctctac ataattaact
     1921 ttattctaat tatcattaca aataacgata ttaagcatac tgcaattgag gaaaaaagag
     1981 ccttatagct ctttccccat tgtagttctt taatactgct tatgatttgt ctaattccaa
     2041 caaatttata aatataggta aacatgataa caatagttat tgtgtaaatt tggaatatct
     2101 gccctaataa gactaaatag aaagcaatca tgttttcttt tatctcttta ttctttttta
     2161 tattgtaaat aacatatgca ctgtatactg cactaaagtt agagattaaa tttaaacttg
     2221 cattaacatc attgtttaaa agttgtgaaa atgaaatatt tgtaaatgat gatgtcaatt
     2281 ctaaatacac tatatataaa acaggtaata ttagaaaaca aaccataacc actgaaagaa
     2341 atatattttc atcttttaat actgctgtga tattttttat tttatttgtt atcatgtttt
     2401 tgttctcctt atttaaaagt tggccagaaa tccttattag ccacaatcat ttcatctaag
     2461 atttcttttg ctacttttgc acttggaaca gttttactca tagtaaacga ctgccataat
     2521 ttttgataac ttttttctat ccatgcttct acagctaatt tttcagaagc aacttgttgt
     2581 tccattaaac ctttttggaa agttggaatt ttacctatag acataggttc aaccatatct
     2641 tttccaacaa ggcaaggaat ttcaaccatt gcatcatctg caaaattttc aatagctcca
     2701 ttattaggaa caatcagtaa aaatctttcc tgagtattaa atgctaatgc acaagctaat
     2761 tcaacaataa attgagaatg ttcacttgca tcaaaccatg tatctcttgc tgtgccattt
     2821 tttataattc tttcacattc ttcgaatgta tccttttctc ttctatccat aatttcattt
     2881 gctcttgtat aatttggatt agcatgttca acagtttcat cagccattaa ataatatttt
     2941 aaataagtat taggtaatgt agttggttct atttctaata agtcttttgc tgctctcata
     3001 gtttctaacc aactagattc tttgtgttga ccttgaggtg taaattcctc atatcctttt
     3061 ttagcacaat attcttgtaa tttaggcatt aaatcatttc cttctttgtc cttaatactt
     3121 gtccaccacc cgaaatgatt caatccataa tatctaacat caaagtcttt acgagaattt
     3181 aaacctaaaa ctctagcaaa tatttcctct aaacatactg gcatatcaca aatattaata
     3241 attcttgaat ttggtcttaa tacacgacat gcttctgcaa ctatagctgc tggatttgaa
     3301 taattaagca tccaagcatt tggagaatat ttttccatat aatcaatcat ttcgattatt
     3361 gaacctattg ttcttaatcc ataggcaatt ccacctgccc cacaagtttc ttgaccaaca
     3421 accccatatt ttagtggaat tttttcatct tgttcacgta aagcatataa accaacacga
     3481 atttgtgcca ttacaaaatc aatatcagta aatgcttctt ctggatttgt tgtataacta
     3541 aatttaattt ctggattttt ctctctaaca attattgcac atgcttcagc aacttttctt
     3601 tgtctctcat cattattatc atataattta attgattcaa taggaaattt agcaacactt
     3661 tcaattaaca ttgttacaat tccagcagta taagtactac ccccaccagc aattagtatc
     3721 ctctgtcttt ttctttccat ctttccttct cctttataat aaattttcaa attcttctct
     3781 tacacttggt actgaaagcc caataataat ctgaatagct tttccattat gcactaaacc
     3841 atgtgcacct actgccttaa aatcttcttc tttagaaata attgagtcat ctttcaaggt
     3901 taatctcaac ctcgtagcac agtttgtaac atctacaaca ttttctgctc caccaactaa
     3961 ttttatgtat tcagatgctt gttgagaggc tttactcaat ttatttcctt tttcatcttt
     4021 agtttctaag tattcatttt tagtatatag ttttgtttct tcactatctt cctctctacc
     4081 aggagttttc atatcaaact tcttaattaa gaaagtaaat acaaagaacc agataaatga
     4141 aaatataatc cctacaacaa cttgaacaac atataccttc cagtgataca ttccaagagg
     4201 aagccaattt aatacaaccc agttaataat tcctccacca aaatcaccaa caactcctag
     4261 ggcaaacgaa atcgtttgca agcatgcatc taaaaatgca tgtactagga ataaaagtgg
     4321 tgctacaaat aaaaatgtaa attctaacgg ctcagtaatt ccagttaata ctgcagtaag
     4381 agtagcagga atcattaaac ctaatacttt tttctttttt tctggctttg cagttacata
     4441 gaaagcacca aatacaccta atgaaccaaa caccttcgat aatccggata atgaaaatcc
     4501 gattggatac atttccttta gagtcttagc tgaagtttga aaatcctgaa tatgagtagc
     4561 ccaatatact gatgtaccac ctggaacaac aacactatca taacaaattg gtgcatatac
     4621 aaaatgatgt aatccagttg gtattaaaat cttttgtaag aatgcataac accaaactcc
     4681 tatatttcca ctatttataa aaaacgtttg caaatgtcta ataacatttt gaatatgagg
     4741 ccaaataaaa caagttaata atgcaactgg tatcataaca aagaaacaaa ttgcaactac
     4801 aagtgatgag cctctaaata atccaataaa atctggcagt tctttatcat aaaatctatt
     4861 atgaatataa atgacaatac ctgatattaa caaagcacca accataccag tatctaatgt
     4921 tttaatacca gcaatgctta caagtccact tccagcactt atttcagctg cgaaatcaac
     4981 tccaaagctt tttccccaaa aacttaacat agaacctaca aaataattaa aagtcaaata
     5041 cgttgctaaa gcctccatac aagctcttgc ttgttgcttt tttgcaagtg ctattggtaa
     5101 agaaatagca aataataatg gcaattgatt aaataccatc caagctccac tagatattac
     5161 accccaaaat tggtaccaaa tattttctgg tgtagcaagt gaacccataa ttgactgatt
     5221 ttgaaataca attgagaatc caaccattat cccagcaaaa gcaaataata gtgttggtgt
     5281 aaacattgcc cctccaaaac gctgtatttt cttcatcata taaattactc ccctcctcat
     5341 atttttagtt tatctttggc cttagatatc tattatttta acaggttaat aaatatatac
     5401 aatctttagg gtacattcgg agcttattca cagtcaaata agtttttgca catttttatg
     5461 ttcaaattct cataaaaaaa aagaatgact acaatttgca atcaatccct tctgtaatca
     5521 ttctatgcct tttataaata ctatatttaa taaataataa ctcaatcaat ataaaatata
     5581 acattgtcaa tttataacta gggtgcattt gtaaaaatga gatattagtc gctgaaatat
     5641 aaattgattc atcacttaaa tcagtcaatg tattattttt aaactctgta attgaaatta
     5701 ttttagttcc tattaatttt aatcttctag caatattaag tgtattctca ttctcaccac
     5761 ttagggatat aataatcata acatcatttt tactgacaat attgtaaaaa gaatcatcaa
     5821 atgttcttcc ctcaaaatca tatattattt cttgacatga taagaacatt ctctttatct
     5881 gttttgcaac cacattttga atatcacctg aaccataagc gtatattgtt cttgattcat
     5941 aaagaagttt acacaatgca tcataatctt tatttttttg atcttcaaca tacttaatta
     6001 ttgaattaca tgcaatatca aatgactgat tttctatgac atgaaccttt tctgattccc
     6061 agcttaataa tactttaaac tcactgaaac cttttaaatc taatttttta acaaatcttg
     6121 taatagttgc acgagacaca ttacatttca tagcaagttc atttattgac atcttactac
     6181 aatgtttttt atttgcagtt atatatttcc atattaataa atctgtatca ttcaatttat
     6241 tataatttaa aaaaatcatt tcttctaatc tcataaacta ttaccccctt taattaaaaa
     6301 tcgtcataaa tattatacca atattcaaca ttaaattaca gcgtattctt atatttacta
     6361 tttagcccat aaaattgact ataaaaaata ctgctataat tgatgtttaa aaaaatgtag
     6421 ttttaatttt attcttttat ctaataatta atattttcaa gttctagtgc ttttcttata
     6481 caattatcat ctttaaaatc agcagtcctt ttacaatctt ttactttaaa ttctggaata
     6541 gttttaaatt cctcatcaca tattccattc ttatttaaat acatacaact tgccattcta
     6601 actattagac cactattttt taaattaaac aatacagggt ctatacaccc tccatctccg
     6661 ccagtagttt caccaactaa agtcgcaaat ttattttctt tacaaaacat tgcaaacgac
     6721 tctgatgatg aatatatttt tctatcaaca agtaagtata tattaccctt aaattttgaa
     6781 tcatctgtag attttatagt ataggaagta tcttcaaaat cattaaaatc ttttataatt
     6841 tctttaggtg cattttcctt tacacttgct ggtaaatttt ctacaggatt taattttata
     6901 tttcttgcat ttgtatattt ttttactatt tcattattat ttctataaat tctataacca
     6961 tttattttga catcattttt tatgagttta gatacgattc cctgccaata cctatcactt
     7021 ccacctaaat tacctcttat atcaataatt aaagctttat gtttttctaa agtgttaata
     7081 taatctccaa ttatttttaa atcattattt atagagccat tctttgatga cattgatggt
     7141 aaatacatat atccaatctg acccttaata acatccttta aaattaattc tttttttaca
     7201 aaaatatcat ctggcatttt tattttaaga ttcatagagt catatctatc cacaactttt
     7261 ttatcattaa gaaaatcata ccaatcatta ttagagtatg atttcttaaa aagttcatat
     7321 cttttcttat tatctattac ttctgtgtgt ttattattta aatcagataa tatagatgac
     7381 agttcttcta taaattcttc atcattttca gtgtttttta ttcgttttgt atattcatcc
     7441 ttatttgcta accaatcaat attattaagc ctcttattta cacctagata tggatatcct
     7501 gtctttacaa cattatacac ataattaaag tcatccacct tttcctcttc agacaactga
     7561 ttcttttgag aataagtaga attacctaat aatattataa atgctaaaat aattactaaa
     7621 aataatactt tctttttcat gtaattctca tccactttca gattttatat tttaatacat
     7681 tgtatcatta ttttattatc acctaaacag ttaaaagaaa ttcttgaaga tatattccac
     7741 tattattcta tttaaaaatt tttatttgta ttttcaaaat tatttcatga aagttataaa
     7801 ataacaatta tattcaaata tctattttac tgctatatcc tgaaagtaaa ttcttatcag
     7861 tgatatacta aaaaagatat gctaaaaaat ttatccatta taatgactta aatttagata
     7921 ctttactgat ttttatttat tagttgagaa agatattgat aattaaaaat attgataact
     7981 atagaaatga ttatttttta tagaaatttt aaggatataa actaattata cttaagtcta
     8041 tatccttaat ttatcatgat tatgagaatt tttacaaata cttatctttg tccgttttag
     8101 aataaaaaca agaacatatc ctgttatttt attcaaacat ccaagtttaa ttactacaca
     8161 attacttatt agaaattgta tctacttcat atatatatct gttaagtata gcttctagca
     8221 tttcttgtct tccagactta ttctttataa ctggcatttg cagtgcataa gattctaact
     8281 ctttaaatcc aaccttacct tctactatat ccttacctat accttcacta aaacttgcat
     8341 atctttcttt agtaaagttc tcaaatacct catcttctaa taatttatga gctattaata
     8401 atccctttgc aaatgtatcc attcctgcaa tataagctag gaataagtct tctacctcaa
     8461 aagatgctct tcttacttta gagtcaaagt taaatcctcc tggtgctatt ccaccttgct
     8521 ttaacacttc atacatagca agtgttgcat catatatatt tgttggaaat tggtctgtat
     8581 cccaacctaa taataggtct ccttgatttg catctatact acctaaaaca ttgtttattc
     8641 ttgctatatt taattcatgt tggaaagtat gtcctgcaag tgttgcatgg tttgcttcta
     8701 tattcatttt gaagtattta tccagattat actttcttaa aaatcctaaa acagtagcag
     8761 tatcaaaatc gtattgatgt ttagtaggct cttttggctt aggctctatt aagaattgtc
     8821 cagtgaatcc tatttcctta gcatagtcta cagccatttg taagaatctt gcaaagttat
     8881 ccatttcaag ttctgtatta gtgttaagta aagtttcata accttctctt ccaccccaga
     8941 atacaaaatt ttctcctcct aagtacttag ttatttccat agctttttta acctgagctg
     9001 ctgcatatgc atatacatct gcattacatg ttgtagatgc accatgaaca aatcttggat
     9061 ttccaaacat attggcagtt ccccataata actttatacc tgtcttttcc atattaactt
     9121 ttatatactc aactatttca tctaatatct tatttgtctc ttctaaatct cttccttctg
     9181 gtgctatatc tctgtcatga aaacagaaat actcaattcc catcttagac ataaattcaa
     9241 atcctgcatc aactttaatc tttgctatct caattgggtc tgttatatta ttccaaggtc
     9301 tctctactgt gcctactcca aatgggtcat taccctctcc acataaagta tgccaatagc
     9361 tcattgcaaa tcttaagtgt tctttcatag tcttattacc taccacttgt tcaggattgt
     9421 agtatctaaa tgctaattca ttgtcagatt ttactccttc aaattttatc tgtcctattc
     9481 ctttaaatat ttcactcatt tcatgtcctc ctaatatttt acacatataa tatatgtagt
     9541 atgttttctt aatttaaaac tattttctat ctttattttt ctttttaagc taatttaatc
     9601 atttattttt gcattaacta cttcatctaa taacttaaag tcatctttca aagatttgta
     9661 tattctttta tacaatctat gatatatatt gtatgtatct acattttcac ttattggttc
     9721 taatttagtt gaagttttta ttatagcatc acatgctttt tgtatatttt catatattcc
     9781 agctcctact aatgcaagaa ttgcaactcc aagtgctggt ccttcacttg aatttatagt
     9841 atatacatct tgattaaaca tatctgcttg catctgtctc catactttac tttttccacc
     9901 tccacctgag gctcttactt cattaacgtc tatattaaga ttctctatta tatccataca
     9961 gtctttaaga ctatatgaaa caccttccat tattgacctt gcaatttctt ttttagaatg
    10021 gattggagat aatccaaaga acacacctct tgcataaggg tcaagatgag gagttctttc
    10081 tcccatcata tatggtaagt aaagcaatcc attacatcct gttggtactt tacttacttg
    10141 attatttata atctcatata catcttcacc taaagaattt gaaacttcca cttcactttg
    10201 acagaaatta tctttaaacc attttaatga caagccagct ccttgagtaa ctcccataac
    10261 atgccatgta tttggtattg catgacagaa agtatgaact ctaccttctt tatcaatttt
    10321 tggctctttt gtataagcaa atactacacc tgatgtacct atagtagatg aaactactcc
    10381 atctttaact attccatttc ctatagctcc tgctgcttgg tctccagctc ctcctaccac
    10441 tattgttcct tctttaagcc ctgtcaaact tgctacatct ctagtcacat aacctgtaat
    10501 ctcttgtgat tcataaactt tacctaaaag atttttatct atatttaact tttctagtac
    10561 ttcatcgctc caatctcttt ttcttatatc gagcaactgc ataccacttg catctgatac
    10621 ctctgtagca tattcacctg tcaatttgta tcttatataa tcttttggaa gaagtatttt
    10681 attagtattt ttatatatat ctggttcatt gtttctaacc cataatatct ttgatgctgt
    10741 aaatcctgta agtgctggat tagcagttat ttcaatcagt ctttcttcac ctactaaatc
    10801 agttatctcc tcacactctt ttactgttcg ttggtcacac catattatag agtttctcaa
    10861 tgttttacct tcattgtcca gcataactag accatgcatc tgtcctgata atccaattcc
    10921 ttttatacat tcactgctta tatttgaact ttgaactact tctcttatag attcgactac
    10981 agctttccac caatcatttg catcctgctc tgcccaacct gatttctctt gtataagagg
    11041 atattcatat gtacaacttt ttatagtatt tcctagttta tcaaataaaa ctgttttcgt
    11101 acctgatgta ccaatatcca cacctaatac atactccaac ttcatccctc cttgaatgcc
    11161 acttcataac tatcgttttc ctatcctaat ttttttatat tttaggtata ttttacttgc
    11221 tttatttttg ttagtatatt aaactaactt atgaactaat aataaaatat ttaattatct
    11281 ttgtcaatgc aaaataacct tttatttttg tgtaaaaagt attacaatat tattttaagg
    11341 aggaatatca aattggttac tgataaatac actattcgag aaatgaatga aagattggta
    11401 cttgaacaaa ttataaaaaa tggtcctatt tctagggcaa gtatagcttc tactatagga
    11461 ttgaataaag caactatatc cgctattacc aaaaaactta ttgatgaatc tctagttcat
    11521 gaaatcggaa ttggaaatag tactcatagt ggcggaagaa aacccatatt attagtattt
    11581 aataaatgtg cagggatttc tttaagtatg gacattggat acgactatat attttcttct
    11641 ctatcttact tagatggaac tattataaat tctaagaaat taacagatat acaagtaagt
    11701 aaagacaatg ttatacaatt gatagatgaa attataaata gttataacat atccaaaata
    11761 gacactcctt ataaggtaat tggtcttact ttggctatac atgggattac ttgtgaaaat
    11821 aaagtattgt ttactccata ttacaatctt aatgagattg acttatatag catccttagc
    11881 aaaaagtatg actttcctat tcatatagaa aatgaagcaa acttaacagc tttagctgag
    11941 aatacttttt caactgtaca taactcacta cttagtctca gtattcacag tggatttggt
    12001 tcaggcatta taataaataa taaattatat agtggcagaa atggtatgag tggagaaatt
    12061 ggtcacacta tcatcatgcc taacggtaaa ctatgtcctt gtggaaatcg tggctgttta
    12121 gaacagtatt gttcagaaaa aaaggtattt gaacagcttt cttctttaga aaatattcct
    12181 aaaatagatt ctgatatagt aaagcaacta tattatgaag ataaccagaa tgccaaaaaa
    12241 gttattcatg agttttgtag ttacttaaca atagctataa ataatgctat tacaacttat
    12301 gctcctgaaa taatatattt aaatagccaa ataattagtg atatccctga gatattacag
    12361 attacaaaag atatgttagt tagctctttt aacaaaggaa taaatataga aatttcatca
    12421 ttagggtctg aggcatctct atatggtggc tctgccgtaa atatcaagag ctttttgaat
    12481 atacaaaatt taactttaat taatgaaata taagggttat gactatatac agccataacc
    12541 ctttaaatta tacattctaa aattagattt attatatagt ttatgcaaga ttagctatat
    12601 tttttaaaaa ttgtatatca attaagtatt aacataagta ttttgtttta aatattaata
    12661 taatcttcct actcatcatc agataataaa cttgcatttt gttcaaccat agaaatatta
    12721 tctttaccta ttttctctaa ttttgctgcc aattcctttg tatctaaaaa agctgattca
    12781 ataaccatac ctaaatttag ccctgaaata attttaattc gactgtcatt attggctact
    12841 aataaagcag cattgcatgg agttccacct agtaaatctg ccattataat tatttctgaa
    12901 ctttccttgt acacttctaa agcttttttt aatttttcta ttgttccttc tattccatca
    12961 tcttgtttca tatttacagt aggataatct atagaatccc ctattatcat ttttgcagaa
    13021 tttaagactt cttcagccat attgccatga ctcatcaaaa taatactatt caatttaata
    13081 cacctcttta aatctataag atgtctacaa gataagtttt cttatcatca ggcgtcatta
    13141 tagttgtaat ttcaattcct tcatctaaaa gtcttttaaa tgcttctttc tcttcttcag
    13201 taacactgat attaggtctt agaactgttg tatcttttct acttgacata tttcctacat
    13261 ttatattatg tattttaaat cctgcctcta caaatggaat aaggtccaca ggagacttaa
    13321 caattaataa tacattttgt ccttcatact ttcctgcagt aatatttttt atagcagtct
    13381 cttctgtaat tatagatgac gctatacctg caggtgtagc cattcttaaa acttgttttt
    13441 gaacagaatt gttagctact gcatcattta ctaccattaa tcttgtaaca cccaatttat
    13501 tactccacat agttgcaact tgaccatgga ttaaacggtc atctatacga atatttttaa
    13561 tacctttcat ttccatatct aatatcatcc tttccttatt ttaaaatacc taatgcatat
    13621 aaaattattg aaacaaccat tactcctaaa attaagtata taggttttat attcttttta
    13681 agagcccaat aaacaatcac aactagaatt gcaggaacta acccaggcat aatttggtct
    13741 aatatttctt gacctttcat tgtaaaatca ccttgttgaa agttcaaagc aacattagct
    13801 tttacaactg aaggtattaa tgcacctaca accattaaac ctaaaataga tgtactctca
    13861 gttaaaatct ttaaactttt ttcaattgaa gatattagct tagtacctga tgtataacca
    13921 gcattaaata gtggtatact taataatttc aatgctacac tcactacaat ccacagtaag
    13981 attccaaatg gacttccatc tatcgccata ttagctgcta tagctccaaa aattgtccac
    14041 ggaataacga caaataaact atctcctaag cctgctactg gtcccatcaa gcctgtttta
    14101 attgaagcaa cagcctcttc agtctcttca cttgataact cctccattgc tacattaata
    14161 cccaaaatat aaggggcaac ccaaggatta gtgttaaaaa attgtaaatg atttaataca
    14221 gcactttgta acttttctgg attatctttg tatactactt ttaaaaaagg tagtactgca
    14281 taacagtatg ccaaggcctg catcttctca taatttgaag aaacagaact agtaaaaaac
    14341 cagcgccata agacactttt tttcaccttt tcatttgatt tatttaatga taccttatca
    14401 ctcatcttcg tccatacctc ctactacagt atattgttgt tcttgatttt ccaaattctt
    14461 tttgtaaatt ataagtgcaa ttgctccacc aattaaagct acacctaata atggtacttt
    14521 catatatact gctaaaacaa aaccaattac taagtaacta aaatactcct tagcaggaag
    14581 atatctaagt aataatccta tccctactgc tggaagtagt cctccagcta ctgttaaacc
    14641 acctgttaac cattcaggtg tatatttcaa aatggtatta attaaagaag gtccaaatat
    14701 tacagataga gcaactggaa tacctgttgt agctgctgtt aatagtactc ctaacatttg
    14761 catataggtt atatttttat aatttccttt tttagctcca tcttctgctt tatgaactag
    14821 ccatatacca atagtatttc taagaacatc taattgtacc atcaataaag caactggaat
    14881 acctatagag attcctacag atgcttcttg cttagtcgta atagttataa atgtggctac
    14941 tatacttgct gtttgataat caggtattga tgctccacca aagttactaa tacctaatga
    15001 taatagctgt aatgtacctc caatatataa ccctgtattt atatctccta agataagacc
    15061 agtaataaaa cctgctgtta ctggttgata tgttccaaac attgttgaat ttttttcata
    15121 gtttataaaa aatccatata gtacgattaa taataattgc cataattgtg gctccattat
    15181 gatttcacct ctcccaaaat attttttagt tctttaaaat tataactgga atcttgcttt
    15241 aggaaaatag gaatttcttc cagtgggcat tcaacttcat aagttttccc accttcataa
    15301 ctaactccac taaatatatt ttcccatgta tgtccttttg gtaaatatac ttttctactt
    15361 ctatctttat aattcattat tggagctacc aaaatttcat ctccaagcat atatgtatta
    15421 tccacctccc aagttttatc atcttcagga aattcataaa ataaagttct cataactggt
    15481 gttccatctt catgcgcttc tttcatcagt ttttttaaat aagtttttaa gctctctcta
    15541 atttttatga attttgtaag aattacttca acttcttcac cataagacca tatttcatta
    15601 ggagctccag ttaccattga gcctccacct ttatttgata atggtttact atgaggcaat
    15661 ctatctccat gcattctcaa aattggtgaa aaagtagcat attggaacca tcttaccata
    15721 agttctctaa attctgggtc tttaggattt cctccatgga aacctccaat atcagtagtc
    15781 caccatggta tacctgccag tcccatattt aaccctgtat ttacttgatt attaaaagct
    15841 tcaaaactag aatcaatgtc accagaccat actaaagctc catacttttg agctcctgcc
    15901 caagctcctc ttacaagagt tacaacagac tctatgtcat tatgtaaacc atcataaatc
    15961 atttttaaat aatcaatagg ataaatatta ccacaactta aaacatttcc ttttttatat
    16021 ctatagttat caaaatcata cacagaatag ccaggttcag ctacatctaa ccaataatag
    16081 tcaatcccaa gttgtttata atttttatct attctatccc aaacatattc tcttgcattt
    16141 tcatttgtca tatctacaaa gactgtattt ccttgaattt gcattgtcat tctaacacca
    16201 cgattaacat tgactaaata gccattttct aaataatcat tgtagttttc tgcatcactt
    16261 tgaactgtag gccatacaga tactattggt tctactgaat actcttcttt caatttttta
    16321 actaattctt taggattttt ccaataatct aagtcaaatt tatattcacc ttgcttaggc
    16381 caatgaaaat agtcaattgc aattgatgaa agcttaattc ctcttttaga atattccttc
    16441 acaacatcta aaacttcttc tgaagttcta tatcttagtt tactttgcca tagtcctaaa
    16501 aggttttcag gcatcatagg aacagttcca gtaacttgag agtaattttt atttaactct
    16561 tttgggcttt ctccacaagt aatccaataa tctataaaat ttgtagaaaa catcttccat
    16621 tctgtcatat ttttagcaaa agatactttt ccaattcccg gattattcca taaaagtcca
    16681 taacctagac tagaaatata aaaaggaact gatatttgag aatttctttg ggctaactcc
    16741 aaaacagtat tctttaaatc taaaaattta tgttgatatt gtcccattcc aaaaatcttt
    16801 tcagaaggct cagattcaaa tctagtagtt acttcaaatt ctccactata gcttggtcga
    16861 tattctcttg attttaactt tagagtagag ttaaagtctt tggtaatctc aattgtccct
    16921 acatcttctc caccatcatc atgttttaca gctcttaatc gaatatattc tttcaataat
    16981 atttcatttt tatcattata aaaagtaatt ctatctctat ggtctaagac agcttttatc
    17041 tttccatttt taatgattgc acttccatct tcatttttat taattgtaat atcatcttta
    17101 tttaacttag gtttttcgtt aagagcatag ttttcatcaa caaaattttg gtctacaaac
    17161 gacctaatac gtaaagagtt ttttccccat gcttctattc gcaatagttc attgtcaaat
    17221 cttttttcaa tatagtcttc agatattttt atcacggcta cctcctaaat caatggtata
    17281 atattctatc tttgtgtagc ttaagttaaa taatgtcgtt tagtatcgtt atttataaat
    17341 taggttttat atataattca tttgtacatt aaactaactt accaataata tactttattt
    17401 gactttgttt gtcaatcgct ttccggattt tagtaaaatt ttctaatatg ttgctggaaa
    17461 gtcgttctta atatctttta tttatacttt taatatctgt gatgatacct atatacatct
    17521 agttttttta aaacacatac aggaataaag tttgcctatt atgtagataa tatcggactt
    17581 gaaaatatgt
//
LOCUS       DAFKMX010000081        17384 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFKMX010000081 DAFKMX010000000
VERSION     DAFKMX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.081"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            16..720
                     /locus_tag="LZB08_002076"
     CDS             16..720
                     /locus_tag="LZB08_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBM7315579.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            812..1858
                     /gene="selD"
                     /locus_tag="LZB08_002077"
     CDS             812..1858
                     /gene="selD"
                     /locus_tag="LZB08_002077"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:860..862,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBM7315580.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            1876..3288
                     /locus_tag="LZB08_002078"
     CDS             1876..3288
                     /locus_tag="LZB08_002078"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBM7315581.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTNTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEYMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            3402..5285
                     /gene="selB"
                     /locus_tag="LZB08_002079"
     CDS             3402..5285
                     /gene="selB"
                     /locus_tag="LZB08_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBM7315582.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            5390..8128
                     /locus_tag="LZB08_002080"
     CDS             5390..8128
                     /locus_tag="LZB08_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBM7315583.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLVSLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(8247..9191)
                     /gene="manA"
                     /locus_tag="LZB08_002081"
     CDS             complement(8247..9191)
                     /gene="manA"
                     /locus_tag="LZB08_002081"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBM7315584.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIIYSHI"
     gene            9364..10725
                     /locus_tag="LZB08_002082"
     CDS             9364..10725
                     /locus_tag="LZB08_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7315585.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKELYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TLEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            10813..12702
                     /locus_tag="LZB08_002083"
     CDS             10813..12702
                     /locus_tag="LZB08_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7315586.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEEGSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            12717..13172
                     /locus_tag="LZB08_002084"
     CDS             12717..13172
                     /locus_tag="LZB08_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315587.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            13231..13548
                     /locus_tag="LZB08_002085"
     CDS             13231..13548
                     /locus_tag="LZB08_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBM7315588.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            13573..14835
                     /locus_tag="LZB08_002086"
     CDS             13573..14835
                     /locus_tag="LZB08_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBM7315589.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GATFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            14940..16004
                     /locus_tag="LZB08_002087"
     CDS             14940..16004
                     /locus_tag="LZB08_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBM7315590.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            16058..17236
                     /locus_tag="LZB08_002088"
     CDS             16058..17236
                     /locus_tag="LZB08_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBM7315591.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7074 a         1724 c         3115 g         5471 t
ORIGIN      
        1 aggagtgatt aatatatgaa aaaaaaggta aaatttattt cgttttttgt tttatttatt
       61 ttaataatat catcaataat cgtttataaa tcgcttattt tcaaagatga tatttatgtt
      121 gtaagtaaaa aaaatttacc taatgaaaaa acacatgttg tagagaatga aattaaaggt
      181 gaagataata atcacagtaa agtaactaaa gacatagata aaaattttca aaataataaa
      241 ataactatat acataagtgg agcagtaaat agacctggaa ttgtaactat agagtcagac
      301 aagagacttt atgatgctgt agaattacta ggaggaacga caaaagaagc agatttaaat
      361 ggagtcaatc tatcagtaag attagaagat gaacaacatt acataatacc taaaataggt
      421 gaagcaacat ctgttacaag taatgatgat tccaataaac taaatcagaa gaatgagtca
      481 agaggtgagc ctaaaaataa atccaataaa aatatatcta acgaatcaaa agtaaatata
      541 aatgtagcga cgatagaaga gttggattct ctacctgggg ttggagaagc tacagccaat
      601 aaaattttac aacacagaga agaaaatgga cagttcagtt caattgagga aataaaaaat
      661 gtaaatggta ttggagataa aaagtatgaa aatataaaag atttaatttg tgtagactaa
      721 aatataatac aaaacttaca tatagatatg ttattataaa tatgtatgta tatgtgtgta
      781 tatgtggaaa tttagtaaag gaggcacttt tatgaaaaaa gaacaaaaaa aacttacaga
      841 gatgacaaca gcaggtggtt gagccgctaa aatagggcca gaggttctgg catcagtttt
      901 atctcaattg cctaaaaatg ataatataga aaatttatta gtaggactag atacagcaga
      961 tgatgctgca gtttataaat taaatgatga tatggcactt atccaaactt tagacttctt
     1021 tacaccaatg gtagatgacc catacgtatt tggtcaaatt gctgcatcaa attcactatc
     1081 agatgtatat gctatgggag gcaaaccttt ggttgctatg aatattgttt gttttccatc
     1141 atgccatgat atggatgtac ttgcagaaat attaaaaggt gggtttgata aagtgaaaga
     1201 aagtggagca cttttggttg gagggcatac tgtagatgac aaagagccta aatatggtct
     1261 ttcagtttct gggattgtaa gtccaaataa agttttatca aatgctactg caaaacctgg
     1321 tgataagtta attataacaa aaccaattgg agtgggtgta ttaaatacag ccatgaaaga
     1381 aggtatggta gaacaacata tagcagataa agtaatagaa ataatgatac atttaaataa
     1441 atatgcagca atgagttttg agaaatttga tgtaaattcg gttacagata taactggttt
     1501 tggtctactt ggtcatactt tagaaatggc caaagcttct gaagtaagta ttgaaattga
     1561 atcaaaacat gtaccaatta ttgaaggtgc aatagaaatg gcacaaatgg gaattatacc
     1621 tgctgggatg tataaaaata tgcattatgt atctaaagat gtagaagttg taggaaatat
     1681 agaagttgca gttcaagata ttttatacga cccacaaaca tcaggagggt tgttaatttc
     1741 agtaaaagaa gaacttgcag aggaacttgt aaaagatatg aaattaaatg gagcaataga
     1801 agctaaaatt ataggttctg tagttccaaa aggggaaaaa tatattacag ttttataaaa
     1861 tattatagag aaagagtgat tgttttgagc aaaagagaat tgtttgcaat gttgccttca
     1921 gttgatgaag tattaagtga taatagaatt gtagaaataa taaatgaata tcctagaagt
     1981 ttagttttag aatctgtgag agaagtaata gatttaaaaa gacaatttat attaagatta
     2041 aaagaagatg catcaaactc tgtaacaata gagtttggtg aaataataga atctgcaata
     2101 gagagagtta agttaaatta ctcattatct ttaaaaaaag ttataaatgc aactggtaca
     2161 gttattcata caaatttagg aaggtcttta cttagtgaag atataaaaga tgaattatgg
     2221 tgtgctgctt caaggtattc aaacttagaa tacgatttgg ataatggaga aagaggttca
     2281 agatactctc atttaactaa tacaataaaa agacttactg gagcagaaga tgtactagtt
     2341 gtaaataata atgcagcggc agtacttctt gtacttagca caatggctaa aggaggagaa
     2401 gcaattgtat ctcgtggaga attagttgaa gtaggtggtt catttaggat tccaagtata
     2461 atggctctta gtggtgcaga attagttgaa gttggttcaa ctaataaaac tcatttgaaa
     2521 gattacaagg aggccattac agaagataca aatgtattga tgaaggttca tacaagtaat
     2581 tatagaataa tgggattcac agagagtgtt tctatagaag aattggttaa tttaggtaaa
     2641 aaatataaac tacctgtaat agaagactta ggaagtggag tttttataga tttatcaaaa
     2701 tatggtttat cttatgaacc aactgtattg gattctattc gacaaggtgc agatgtagta
     2761 acttttagtg gagataagat gcttggaggt ccacaagcag gtattatagt tgggaaaaaa
     2821 gagtatatag aaaaaatgaa gaaaaatcaa cttactagag cacttagagt tgataagctt
     2881 acaatatgtg ctcttgaagc aacattgaga atgtatttag atgagacaaa agctatagaa
     2941 aatataccta ctttaaaaat gttgacatat aaaattgaag aattagaagt aaaagcaaat
     3001 aaactatttg aaaaaataac tgctttaaac ttaaatgcaa acataaatat agaagatgga
     3061 ttttcacagg tgggaggagg gtctatgcct cttgaaacta taagtacaaa ggtaatatca
     3121 ataactcctg aatatatgaa tgtttcatct ttagaaaaaa aactaagact tagtgaagca
     3181 catataatag ccagagtgta tgataataaa tatgtactag atgtaagaac tatatttgat
     3241 gatgaatttg atgtaatagt tgaggaattg agaaaagcat ttaattaaat aaatttgagt
     3301 taataaatat taaatttaga agtataagta tgaagtttta attaaaaaat agctacacac
     3361 acttgtgact ttagtcatag caggtttaga gggggcaaaa aatgaaaaat gtagtaattg
     3421 gaacagcagg tcatattgac cacggaaaaa ctactcttat aaaagcattg actggaagag
     3481 agactgatac gttagatgaa gaaaaaaaga gaggtatttc aataaactta ggatttactt
     3541 attttgattt accaaacaat agaagagctg gaattgttga tgtcccagga cacgaaaaat
     3601 ttataaaaaa catgttagct ggagcatcag gaattgatat agttttactt gttgtagcag
     3661 cagatgaagg ggttatgcca cagacagttg agcatttaga tattctaagc tttttaaata
     3721 tcaaaaatgg aatagtggtt ctaactaagt ctgatactgt agatgatgaa tttagagagc
     3781 tagtaaaaga agatatttgt gaaaaaataa aaggaacttt tcttgaagat gctgaaataa
     3841 ttgaagttga ttctatatct aaaagaggta tagacactct tattgaaaaa atagataaaa
     3901 tgagtgatga tatagaagat aaaaatgaaa actctccagc aagacttaat atagacagag
     3961 tattttcaat aaaagggttt ggtactgtaa taacaggcac tttaatagaa ggaaagataa
     4021 gtatagaaga tgatttggtt atatatccac aggtattaaa aacaaaaata agaagtatac
     4081 aagttcatgg agaaaataaa gatacagctt atgcaggtca aagaacagca atcaatatat
     4141 caaatgtaaa agtggatgat ataaaaagag gagatgtttt agctgcacca aactcattgg
     4201 aggaatcaat gatgttagat gtaaaattat cattggtaaa tcatttagat aaaggattaa
     4261 agcactggga tagattaaga ttgtatcatg gaactagaga gatactttgt agagcagttc
     4321 cactagataa agaattaatt gaaagtggag aaagtggcta tgttcagttg agacttgaag
     4381 aaagcatagt atctaagaaa ggtgatacgt ttgtagtgag acgttattct ccaatggaaa
     4441 ctataggtgg aggtgtaata attgacccat ctcctaaaaa acataagaaa tttgatgaaa
     4501 aagttataga agcattaaaa ataaaagaaa aaggcgaact aaaagatata atagaagagt
     4561 acttaaaaag gaatttaaaa aattatccaa atataaaaga aataatgagc tatagtggtg
     4621 ctcatgaaga agatgtaaaa agagcgcttg aaacacttat atcagaagat aaagtattta
     4681 ttataggaaa tatgtatatg catataaatc aatacaataa attaaaagaa aatacaataa
     4741 agcttttaag tgagtaccat aagaagtata ggcttagaaa agggatacta aaagaagaag
     4801 taaggtctaa gatagagagt aattttaaga ctagggagat ggatatttta ttagaaaaat
     4861 tgtctacaga aaatgctata aaaatagaaa ataatatagt atctctatta gattttgagg
     4921 ttattttaaa tgataagcaa aaagaaatag caaaaaaaat agaaaaaaga ttaaaatcat
     4981 gtggagtttc ttctatactg acaatagatg aagtttctga aggaaatcat aactatgctg
     5041 aagtattgga gtcaatgata ggtaataaag tagagaaatt agatgatttg tatataatgg
     5101 acaaagatat ttatgaaaat gcaaagaaca ttttgataaa ttatataaaa gaaaataaag
     5161 aaataacatt aggagaatac agagatttga ttgattctag cagaaagaat tgcatgatta
     5221 tattggaaaa cttcgataga aacaaaataa ctaaaagagt agaaaacaaa agaattttgt
     5281 tttagcagaa aatgtctgtt tttgctttaa ggtataaaga aggaattata atgtcgagta
     5341 tcgtataatt aattagtata aaatattaat taagaggaag tgtaaagtat tgaagaatat
     5401 aatacctgat gtaaaaccag atataaaaga aagagaagac atgagccttt tatttataat
     5461 aatgggctca gtacttttat tatttattat aaaagatata gagaaatatg aaactttatt
     5521 acatgccata tatacgatat ttttagtagc tacaggagta attatattta atactacaaa
     5581 atttaaagta gattcatttt ctacattttt aggattaatt tttatagcta ctggagtatt
     5641 ggagtgtata tatttattta attccttggg gattaaaact aagtctataa tggagattaa
     5701 catcacaata tcagcaataa cagatttatt tcctatactg ggtgtatact taagttttaa
     5761 atttgttaag gataacaaac aaatttattc aagtgtaatt ttatttatag caactgctac
     5821 attaacaata acatctttat tcataatatc aggaatttgt gactatctgg gaaatggtaa
     5881 atcacaatat gctttaggag ttataatatc tatattcata ataattattt ctatcattag
     5941 tggaattgaa ttaaattcaa gttcaaataa aggtaaatgg gaatatgatg aaaaaaagat
     6001 ttttaacaga ataatagtaa taataatctt atcaagagtt ccaaatctat tgcatatagt
     6061 tattgataat agaaatgtag agaggatatt atcacaaata ataataaatg tagccttata
     6121 ttatttatat aattacatag tatctaagaa tattaagaaa acagcactgg aattgcatga
     6181 tacgaatgaa gaattaacaa aaaaaacgga aagtttaaaa gaaaagaata aaaaattaat
     6241 ctatgagaca gaaaagatag aggaacttaa gggaatactt atgaaaagag aatctaggct
     6301 acaatctaca ttagatgtag cagtaaatag catagtagta tttagcaaag atggagaaat
     6361 tacatatgca aataagagct ttagaaatac ttttggagac tataaagaac aagataaatt
     6421 gactgataaa ataaaaaact tcaatgattt gatagaaagc attcattatg tgtttgttca
     6481 tgagaagaat gcagagaaac ttattagtac aagcgataat aaagtttatc aagctatatt
     6541 tgcaccttta attattgctt cacaaacaga aggagtattg tgtgtactag aagataagac
     6601 taagaaaaag gagtacgaac gcaaacttat tgaagcaaat aagagaagta aagatttttt
     6661 agaaagtatt ggagatggca tagtagtgct tgaaggtaat aacaaaatat atgttaataa
     6721 tgcatgtagg gaaattttta aaaataattt agagagtatt gatttttcat tagtatgtaa
     6781 aagtgaagaa agtatagaaa aaagatatgt aatagatggt gaagtaaagt atgtagagat
     6841 gagtttttct cagtatacta atgctggtac taataaaact attattgtaa tacgtgatac
     6901 aacctctcga aaaatatctc aaataaaact tgaggaaagt caatcatcat attcaagatt
     6961 tatagatata ttacctgatg gcatatgctt attaaaagaa gatttaagta taaactatgc
     7021 aaataagtcg tttttagata tgctagcttt tacaaacata gatgatataa aagattctaa
     7081 tattaaacta ttaatgaatg cgaatactga agaaaaaatg aaatttacgg ataatatggc
     7141 taaagtttta aatgaaaata gaagtatact tttactggaa tatgaattga taaatagtta
     7201 tgatgatata gtagaagtag aattaagtgc attacctttt gctatataca atactagata
     7261 cataatgctt attataaaag atttagtaca taagaaatat tccgagcaag cagaaaaaga
     7321 actgcttgaa agatttaaaa cagataaaat caaaacggag ttttttgcaa atatgtctca
     7381 tgaactaaaa actccactaa atgttatatc tagttcaaat caactggtag attctttcta
     7441 tagaaatgaa aagataaaag attacaataa caatattaaa tctcatgtgg atttagttag
     7501 acaaagttct tataggcttc aaagattaat aaataatatt atagatttaa caaagatgga
     7561 gtctgggttc tatacactaa aacttgcgaa atacaatatt gtatctgtaa tagaagactt
     7621 atttatgaat atagaagagt atgcacttag aaaagacata aaaattcttt ttgatacaga
     7681 ccttgaagaa attaatgttt atatagacaa ggttgaaatt gagagaatta tgttgaatct
     7741 attgtcaaat tgtattaagt ttactgataa tggaggctgg atatatgtaa gtattcatta
     7801 taaaatagat aaagtaatca taagtgtaaa agacactgga gttggaatac cacaagataa
     7861 attagaattg atttttgaag aattttcaca agttgataag actttatcca gaaatactga
     7921 aggaagtgga ataggacttg ctatagttaa gaatctagta agtcttcatg gtggagatat
     7981 agaagtagta agtgaagtga ataaaggcac agagttctta gtttcacttc caatgaaggg
     8041 atttagtagt gaacattaca cagaagataa gagaatatat aatattcaag aaaaaataaa
     8101 aatagaattt tctgatatat attattaaaa atgattatga gcaaaaataa aataaaaata
     8161 ctatttgttt aaatatttaa gttgtaagaa ataggctatc ataaggtaaa ttattatgat
     8221 agcctatttt tagctaggtt ttatatctat atgtgagaat atataatctc taaattacct
     8281 tcaaaactaa aatctccaaa gtcgtttggt agtataaaat gcataccctt ttttaaatta
     8341 tattctacag aattgtgtat aagttttcca tttccatcca acacactgta taaagaaaaa
     8401 ggtgtattgt gtgtaaaatt acattttcca aaaacatcta atttataaac actaaaaaat
     8461 tcgtttgaca caaatgttgt gcatttaaag tctgatgttg atacgatttt atagtcagta
     8521 tcgaaattta tatgaggaac attcgtaaca tctattgatt tttgaacatg aagttcacgc
     8581 ttattacctg aattgtcagt tctatcataa tcatagactc tataagtagt atctgagttt
     8641 tgttgagttt ctaaaatcaa tgttcctttg catatagcat gtatagtacc acttggaaca
     8701 tagaaaaagt ctcccttttt tatgttaacc tttctaagta gtttgtccca ttcattatta
     8761 tttaccatat ttacaaattc tttatgagat tttgcattat gacctattat aatttctgca
     8821 tcatcagaac aatctattac ataccaacac tctgtcttac ctaactctcc attttcattt
     8881 tttttagcat attcatcatt tggatgaact tgaactgata aattatcatt tgcatccaat
     8941 atttttgtaa gtaaaggaaa cttatcacca ggtgtgtttc caaataattc tctattttta
     9001 ttccacaatt cagatagttt tttaccttta tattttccat tttctattaa gcaatctcca
     9061 tttttatgag agcttatagc ccaacattca ccagttgttg gcgaatctat ttcataatta
     9121 aatttatctt taagtgcagt accaccccat attctatcca taaaaatagg ttttaaaaat
     9181 aatggttcca taattctgtt cctcctatga tattaaattt atcctacaaa taattatata
     9241 taaatagatt ataaaatcaa tagtaaaaaa gttttgtccc ttgaaatgac ataaagtata
     9301 aggagttatt aaataagata aactatgata tataatataa agttctaaat tggagtgata
     9361 attatggagt tgtttaaggc agaaagcaaa aaatatgaaa aagaattttt aaaattattg
     9421 gagcaatggg ttagtatacc ttcattttat gatagaaaaa ctgtatctaa agatatgccg
     9481 tttggaaaag gagtatatga tgcattaaac tggtttgaaa atctaggtag agaaaacaat
     9541 ttcaaagtaa aaaatataga taatcatgct gttcaaattg aatatggaaa tggtaaagag
     9601 tatgtagata tatttggaca ttgtgatgtt gtaaatccag gtgaaggatg ggatagtgaa
     9661 ccatttaaac taaatattat aggtgataaa ttagttgcta gaggtgtatc tgataataaa
     9721 gggcctatga tagtgaattt tttagcacta aaaatgataa aggatttaga tattaattta
     9781 aaaagaaagg ttagattaat agctggggga aatgaagaga gtggatttaa atgtataaag
     9841 cattattata gcaaagagct atatggtgta tgtggattta ctccagatgc taaatttcct
     9901 gtattaaatg gagaaaaagg tggagcaata ataaagttaa tttcaaatat agacgataaa
     9961 tctttatata taagtggtgg aatagagttt aatacaatac cagataaagt ttatataaag
    10021 aatgtagaaa aattgggtaa agataatata tgttttgata ttaacaatat atcgataaat
    10081 tatgataatg gcaattatat tgtgtatgga aaaggtggtc attcatctaa acccgaaaaa
    10141 tcaataaatc ctatattagc tacaattaaa cttttgtcag aaaatattga tgaaaaatgg
    10201 acaaaagatt tatataagtt gataaatcaa gataatataa atggaaactt atttggttta
    10261 aatattgagg ggaaatgtgg gattttatca atggtgccaa caataataaa tatagtagat
    10321 ggaaaattag aagtagtttt aagcgttaga tatccagaaa tattaaccct tgaagatata
    10381 ataaaaaaat ttaatttgta tatggagcaa aataatatta ataaatttga gcttattggg
    10441 gaaaatttaa agcaagcaaa ttatatagat agaaattcaa aacttgtaag aagtttgcat
    10501 gatatatata taaagtattc tggagattta aaaaatgatg ttagagtaac tagtgcagga
    10561 agttatgctt ctgaaatgaa taattcagta atatttggtt gtgaatttcc tgatggtagc
    10621 tttggcaatg tccatagtgc gaatgaattt gctagcttag atagatttat aactgcaata
    10681 ggtatatatg cagaggcaat aattacatta tgtaataaaa tataaataca aaatgtccct
    10741 tatatagaca tattatatat acaattaaaa ttttacttga tatataattg aaatcaaaga
    10801 gaaggtgata caatgataac taagaaacat gcaataatag taaagagttt aagcaataaa
    10861 gatggatata tgacatcaaa cgaattagct atcaaattgg atgtgtctac taaaactatt
    10921 aaaagatata tagcagattt aaatagtgtt ttaagcaagt atgatttggt aatagcttct
    10981 tctagaggta taggatacaa gttaagcggt tctaaaaaca atatagccag agctattaaa
    11041 gaagcaaata aatatattga tggtttttta gatgactctg aagagtcaag aatgtcaaat
    11101 ataatatgta tgcttattaa tagaaattat atgagtatag aagcaatggc agaagaatta
    11161 aatctaagta tagccgctat aaataaattg tctagtaagc ttaaaagaaa acttgaaaag
    11221 tatgacttag ttataaaatc taaaccttac tatggaagct atatacatgg tgaagaaatt
    11281 aatataaggc aattaattac tgactatgca ataaaattag atgaaaaaag taaagtgaag
    11341 gtgtttttag atgacattag tgaaaacgat atacactgta tagaaagtat tttagaaaag
    11401 cacttaagag aaaaagatac aataatttca gataaagatt ttaatctttt gctttcaaaa
    11461 ataatcgttt ctgtatttag gagtaagaga ggtcatagta acaatataaa cttaatggat
    11521 acaagttata gatttcataa ctactacttt atagaaaatc taatgaaaga aatatctgat
    11581 aaaattggat ttaaacttat tgaagatgaa gtaatttata tttcaaatta ttctggtgta
    11641 atagcctata aaggtacaca aggaagaaaa aacacaagtg aaattgagga acgaataagt
    11701 agtgttataa gtagtgcact tcaagatata ttccttattt caggtagtga ttatacaaaa
    11761 gatgatgagt ttatggttgc aatctctgac catataaaga gatttttaaa tagagctaga
    11821 gctaatgtaa aatcaaataa tcctttactt catcaaataa aagagaagtt tcctatagct
    11881 tttaatttag ccgtgttttt atcaaataaa ctagaaactg agtttaattt aaaacttgat
    11941 gaagatgagc ttggatatat agctatccac tttgctgctt caaatgaaag aatgaaaaaa
    12001 aatactagta agaaaatttg tatagtttgt cattatggga taggaacagg acaacttatt
    12061 tctgaaaagc taaaacagaa tataagtgat ttaagtgttg taggtgttta tccagttaga
    12121 tatctcgata tggctataag tcaagatgtt gatttgatag tatctaccgt tgaattaaaa
    12181 ggatatgaaa aacctgtctt atatatagaa aatatatttg atgatagctt aattgaaaac
    12241 gtaaacaaag ccttttatga aaaggaagaa agaagaaaga taataagcaa catgtttgat
    12301 gaaaaagctt ttttcagtat aaaggctagt actaaagaag aggtcataat ttcactttca
    12361 aataaactaa aagaaagaga ttttatagag gaaggttcta taaaaagtat aatagataga
    12421 gaaaatattt catcaactga aataggaaat ctagtagcaa tacctcacac aatagtgaag
    12481 ggagataaaa aatctataat aggagttgga atacttgaaa atccaataat ttgggataag
    12541 caagaggttc agctagtatt tatggtattt tttaatacta aagaaaaaca taatttctcg
    12601 atatttaaat acctttataa ttttataaaa gatgaaggag gagtaagagg aattattaaa
    12661 atatgtgact ttaataaatt aatggcactg ataggtaatt aaggaggaaa ttaataatgg
    12721 gaacaaacat atttaacaaa gaatatgtat ttttaaatgt tgatgctaag tcaaaggttg
    12781 aagttttaaa atttatatca aaaaaagcta aagacttaaa cttggcagaa gatgagagct
    12841 tagtatatga aggtttaatg gctagagaag aacaatttac tacaaattta ggagaatcta
    12901 tagcaatacc tcacactaaa aatgatgcaa ttgaaaatcc agcagtagta gtattgaagt
    12961 ttaatgaaga tgttgtttgg aatgaaggtg aggataaggt taaattagca ataagcttac
    13021 ttatgccagg aaaaagtaaa gagaatattc atcttaaatt attatcatct ctttcaagaa
    13081 aattaataaa taaagaattt aaagattctc ttttaaaaag tgataatgtt gaagaaatat
    13141 ctaacttaat aaatgaagct ttaggtttat aattaaacac taaggttata aaataaaaaa
    13201 ataattaact aaaaatagga ggaatttgaa atgaataaaa aattagtagc attatgtgct
    13261 tgtccaatgg gactagctca cactttcatg gcagcagaag caatagagca agcagcaaag
    13321 gcattaggtt atgaagcaaa agttgaaact caaggtgctg atggtgtaca aaatgagtta
    13381 acaagagatg atatattagg agcaactatg ataatacatg cagtagcaat aacaccagaa
    13441 ggtatggaaa gatttgatgg ttgtgaagtt tatgaagttg aattacaaga agctataaaa
    13501 aatgcagaag gtgttataaa agaaatagaa gaagatttag gaatataata aatttattgt
    13561 ggaggaaata gaatggcaat taagaaaaaa gttataaaca gttcaggtgc ttctacaaca
    13621 ggaggaaata atgtaggaaa atctactcca tctagtccaa ctcaatcaaa gggaaatgga
    13681 aaatggaaag aaataagtaa acatataatg acaggtatat catatatgat accagtttta
    13741 gtaatgggtg gacttatagg ggccttatct caattaatac catatgctat attaggactt
    13801 gacccatctg taggtatagt tgatgctatg aactcaggag aatttacagg gttcaagctt
    13861 agtcttttaa atatagcaca attaatgtca aatttcggat ttactttgtt tggatttgcg
    13921 ataccactgt ttgcagcatt ttgtgcaaac tctataggtg gaaaaacagc tttaattgca
    13981 ggatttatag gtggatatat agctaataaa cctgtaggtg taccacaatt cgttgatgga
    14041 caatggactg aagttgttcc agttgcatca ggatttttag gtgcaatact aatagcattt
    14101 ataataggat attttgtaaa atggttaaat aaatctataa aagtttctca taactggtta
    14161 gcttttaaaa caacattttt aataccatta atagcatcat tagcttgtat ggtattgatg
    14221 atatttataa taactccatt tggtggatta attaatgaaa gtatgaaaaa cttcttaaca
    14281 gcagcaggag cagctggaga atatgtatat gcaacagctt tggcagcagc tacagcattc
    14341 gacttaggag gaccaataaa taaagcagca ggattcgttg cacttggttt aactacagag
    14401 aatgtacttc caataacagc aagaacaata gctatagttg ttcctccaat tggattagga
    14461 ttaacaacat tattagataa aagattagta ggaagaagag tatatgatag acaattctat
    14521 caagcaggaa aaacttctat attcttatca tttatgggta tatctgaagg agcaatacca
    14581 tttgcacttg agagaccagg ttttgttata cctttaaaca tagttggaag tgtaatagga
    14641 gctattacag gtataattct aggagcaatt caatggttcc cagagtcagc tatatgggca
    14701 tggcctctag tagataactt atttggatat ataataggta tagcagttgg tgctacattc
    14761 atagcagtag gaaatatatt ctatagaaat aaattaataa aagatggtaa acttgttgta
    14821 gattatatcg actagtattt aatttttact atctataaat aactttggct ggtaatatgg
    14881 ttttaatcaa tattaccagc cttacctata tgattaatta aaggcggtgg agataagata
    14941 tggataagat agaaaaagta agagagtatt ttagagaata tgatatagat ggatttctta
    15001 taaatagtcc tacaaacaag ttttatgttg gaaatttatt tagctcttca ggttatgttt
    15061 ttataacgaa agaatcacaa tatataatag tagattttag atattttgaa gaaattaaaa
    15121 gaaaatctag tttgtttaat gtagtattaa tggacaaaac tagaactcat tttgatataa
    15181 ttaatgatat ttgtagagag cagaatataa aggaaattgg atttgaggga aatgaagtat
    15241 cttttgattt atatagaagt atgagtaata agttaagtgc aaccttaaaa tcagttgatt
    15301 tatctacatt aagggaaaca aaaaatgaag acgaaataaa atatataaaa aaagcttgtg
    15361 aaatagttga tgctacattt tatcatatag tagactttat aaaggttggt atgactgaaa
    15421 agcaagttga aaatgaaatt gttagaatta taaaagattt aggtggtcaa aaagaatctt
    15481 tcgatacaat agttgcatct gggcttagag gagcattacc tcatggaaaa gcaagtgaaa
    15541 aagttataga gtatggagat tttgttacct ttgactttgg tgctaagtat aataattatt
    15601 gttcagatat taccagaacc atttgtatgg gtactataaa taaagagtta gaagaaatat
    15661 ataatatagt aagaaaagct aatgaagagt gtataagggt tttaagaccg ggaatgacta
    15721 ctggagagat agataaagtt gcaagagaca taattggttc atatggatat gccaataatt
    15781 ttggtcataa tctgggtcat ggtgtaggaa ttatggttca tgaatatcct gcattagctc
    15841 ctgaatcaaa tgaagtatta aaagaaggaa tgattgtaac tatagagcct ggtatatatg
    15901 ttccaagtct tggaggcgta agaatagaag atgatgtact tataacacaa gatggatgta
    15961 tgagattgac tacatctact aaggatttaa tagttgtaag ctaaaaataa attagataaa
    16021 tattttataa caataagaaa agaagaggta aatttaaatg tataattttg atgataaacc
    16081 agatagagta agtgaaaagt gtagaaaatg ggatttaaat attataagag ataaatttgg
    16141 agatattaga gaagattttg taccaatgtg gattgctgat atggatttta aaataccaac
    16201 agaaatagaa aacaaattta ttgaagctgt aagaagaggt gtatttggat atacatactg
    16261 ttatgatgaa ttttatgatt cagttattgg ttggcaaaaa aatatgcatg aggtaaatgt
    16321 agaaaaagaa tggattacat taacttatgg aactgtatca acacttcatt atgtagtaca
    16381 agctttttgt aagaaaggtg atagtataat attaaacaca ccagtttatg acccctttga
    16441 atcatcagca aaaaaacaag gtgtaaatgt aatttgtaat acattagatg ttgtaaatga
    16501 tagatattat ataaattttg ataaattaga agttcaaatt aaagaaaata agcctaaatt
    16561 aatgatgttt tgtacaccac ataatccatc tggaaggata tggacaattg aagaaatgac
    16621 aagggtagca actatatgta aagaaaataa tgttattttg gttgcagatg aagtacatgc
    16681 agagcatatt cattatggta aatttaattc aatacttaag attggaaaag agcttttaga
    16741 aaatgttatt ctattgacat caccaaataa aggatttaat cttggtggat taaagacttc
    16801 ttattcaata gttataaata aggatataag ggacaaattt agaaataaat taaagcaaaa
    16861 ttctataact tctccaaatg tgtttggaat aataggattg ataaccgctt ataatgaatg
    16921 tcatgagtgg ttaagtggtg taaatgaata tataaaatca aattatgaac ttttagaaac
    16981 ttgggtaaat aaatataata agataaaatt gatgaaaatg gaatcatctt acttagcttg
    17041 gatggatata agtggattag gtattagtgc aagtgagttt acagataaat tggctataga
    17101 tacaggagta ttattagaag atgggagtca ttttgtaaaa gatggtgaga agtatgttag
    17161 aataaattta ggaactcaaa aggaaaatgt aattgaagct ttaaatagaa tggatttatt
    17221 tttaaagtct ttataaaaat ttggttgaat tagtatttta tataaattta aactattaat
    17281 atggtatata cataagtaga atttgtttta gattacttaa gtatatacca tattttatat
    17341 aaaaattaaa tttgcattag tcatgtgata actatttaaa ttca
//
LOCUS       DAFKMX010000082        17252 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFKMX010000082 DAFKMX010000000
VERSION     DAFKMX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.082"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(124..648)
                     /locus_tag="LZB08_002089"
     CDS             complement(124..648)
                     /locus_tag="LZB08_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBM7315592.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="LZB08_002090"
     CDS             complement(713..1465)
                     /locus_tag="LZB08_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBM7315593.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="LZB08_002091"
     CDS             complement(1496..2794)
                     /locus_tag="LZB08_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBM7315594.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="LZB08_002092"
     CDS             complement(2811..5075)
                     /locus_tag="LZB08_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBM7315595.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6148)
                     /locus_tag="LZB08_002093"
     CDS             complement(5330..6148)
                     /locus_tag="LZB08_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBM7315596.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="LZB08_002094"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="LZB08_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBM7315597.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7126)
                     /locus_tag="LZB08_002095"
     CDS             complement(6842..7126)
                     /locus_tag="LZB08_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7315598.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7116..7868)
                     /locus_tag="LZB08_002096"
     CDS             complement(7116..7868)
                     /locus_tag="LZB08_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBM7315599.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8050..8242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8374..10146)
                     /locus_tag="LZB08_002097"
     CDS             complement(8374..10146)
                     /locus_tag="LZB08_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBM7315600.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10160..11089)
                     /locus_tag="LZB08_002098"
     CDS             complement(10160..11089)
                     /locus_tag="LZB08_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7315601.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11317..11505
                     /locus_tag="LZB08_002099"
     CDS             11317..11505
                     /locus_tag="LZB08_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7315602.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11498..12199
                     /locus_tag="LZB08_002100"
     CDS             11498..12199
                     /locus_tag="LZB08_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315603.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12201..13124
                     /locus_tag="LZB08_002101"
     CDS             12201..13124
                     /locus_tag="LZB08_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7315604.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13281..14204
                     /locus_tag="LZB08_002102"
     CDS             13281..14204
                     /locus_tag="LZB08_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315605.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14197..14934
                     /locus_tag="LZB08_002103"
     CDS             14197..14934
                     /locus_tag="LZB08_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315606.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14945..15718
                     /locus_tag="LZB08_002104"
     CDS             14945..15718
                     /locus_tag="LZB08_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315607.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16259..16867)
                     /locus_tag="LZB08_002105"
     CDS             complement(16259..16867)
                     /locus_tag="LZB08_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBM7315608.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17045..17161)
                     /gene="rrf"
                     /locus_tag="LZB08_002106"
     rRNA            complement(17045..17161)
                     /gene="rrf"
                     /locus_tag="LZB08_002106"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5548 a         2991 c         2187 g         6526 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata catcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgagtg accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 tatcttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca tatatgaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tatgacttta
     4201 ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttctaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttggtact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttatcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt ttgttttagc actcattagc tttaactcct
     7141 ttatcattat tttctagtat gcttttttct ccgttttctg taattaaatt atatacaatt
     7201 acagtaagta taccttctgc tattgctatt ggtatttgag tcataaagaa aattcctaaa
     7261 aatttaattg ctgaagccat taccccacct gatgggtctg ggaaagccag tgctaattgt
     7321 attgaagtta ttgtatatgt ggctaagtct cctattgctg ctgcaagaaa tactggaata
     7381 gcattatttc catctttttt ctttaaagcc ttaaatatta ggaatgatac gattggccct
     7441 attatcgcca ttgaaaaagc attagctcct aaagtagtta ttcctccatg tgctaataac
     7501 aatgcttgga atattaaaac tatagttcca agaacaaaca ttacgcttgg tccaaacatt
     7561 attgctccaa gacctacacc agtaggatgt gaacaacttc ctgttactga aggtattttt
     7621 agtgctgata atacaaacac aaatgctcca cataaagcta ataatacttt ctttttaggg
     7681 tcttgcttta ctattttacc tattgatttt aatccgaata agaaaaatgg tataaatata
     7741 actccccatg ctatagacca ttttactggt aagtaacctt ccattatatg cattgcattt
     7801 gatgtaactg gagttaaaat gattaacata agtgcagcta taacccctaa tttaatattc
     7861 tgtttcatct tagtatccct ctctcccttt ctaataatta aaacataata atacatgtct
     7921 gatttattta ataaaataaa aaagcctaac tcgatggtta ggcttctaat tttccacaat
     7981 ctaaatacaa acacataaaa caaacatatc tgtataatta ttataagaaa ctttctccat
     8041 ccccgaagaa attactttaa tattttaggc aggtctcctg acttagcttc atcctactcc
     8101 ttttccttcc catgagatat aaaaaatatc atcatagtgg ctaacaaaag tttcgtctgc
     8161 atcacagtag cgggggctgt aaaggaattt aacctttttc cctattaatc ttgcttaaag
     8221 aacctataat attatatatt taatttttaa attattctta aatcattttc tatataaatc
     8281 attgtatacc ttttctgtga aaatttctac attttttata aattttattc atttttatat
     8341 ttttatgtat ataaattctt ttttgtaagt ttattaattt ctaattatct tctttaaata
     8401 cattgaaact acatcagaaa atctagttat atctcttgaa tatataaaat catttccata
     8461 tattaatctt tcagcttcaa gttctgattc ttttcctgta aatactccta atactaatat
     8521 attttgcttt ctagcttttc tcacttcatt ggctgtatct ctcaaaccaa ctatacccct
     8581 ataagccttt tctcctctta atgttctctc actacttttt cctatcttta catcatttgg
     8641 tcttccatcg ctgagaacta tcaggatttt attttcttca tctctattta ttagaccact
     8701 acatatagat tttattgcca aaccatctct attattacca gcacaaaaat attcaaatat
     8761 attttcacta ttttttatac tatcatcata atctttaaat ctttttaata ttgtatagtc
     8821 taaaaaacta gaaaatccca tcactctatt tggtatgcca acttctgtta gagcatttgc
     8881 aattatgtaa gcttgaattg ccacatttcc ctgatttcta ctttgagagc cacttgcatc
     8941 caataatata tcaataacgt atttcccttt ttcattttct atatctttat aaaatatttt
     9001 attattatta cttctaccaa ctctccaagc tctattggct aatatagtac cacaatcaga
     9061 atatactcta cttttagcat tttcttctat taaaattctt gacagacttt cttttaatct
     9121 ctttatttgc tgtctataca ttctagcatg ttctctaaat tttcctaaat tattttcttt
     9181 ttgtcttgtt acatatttta gttgaaatac attattacac tcacttctaa gaactccatc
     9241 tgtaaagtgt attctacaac cttcatgaac atttctacaa ttttgtgtct caattttttt
     9301 aatttcacct ttactcaaat aagtttttcc ataataatgt tctatttttg aatatatctt
     9361 atttgcagtg tcttcatcaa catatataac tcggtctgct gaattgctac atttagaatc
     9421 atcaaaatcc cctacacctt ctacaagcat ggatgaagat attgaatcta tatcactttc
     9481 aataatattt tcttcttcat cactataaag ttcttcatac atgaaatcag caaatgtatc
     9541 aaaatttact tctacacttt ctacattatt ttcatcaata agactatttt cttctccatt
     9601 taatatgtat tcaaaataag ttaaatatat aacatctaat gcttttaaaa tctccataat
     9661 atcttgattg gtatctatat ttcttatatc attaactatt cttctagtca tactatctaa
     9721 tgctgggtgc ttgtccatac tataaagtac aaatgtgtat ttaattttat ccataatatt
     9781 atcattgtgt attttggtaa aacgtgaaac aatatcttta taagcatttc ttcttatgtc
     9841 ttctatccct ggcctgtctt taatgacctt ttcttcaact attgtattta atataacttg
     9901 aattagacct agtataacat ctttgttgta tccttttctt attctatttg taagatattt
     9961 tttaactata tcccaatcta catactttct tctagcacct gcaattattg cgaaatatag
    10021 acctatttct tttgaagagt aatctttttc tgatatatct atactttcat tgtaatcacc
    10081 agctattgtc catgataggt tattagctct attttcaaat tcataatcat catatatcca
    10141 tttcataatc tcacctatat taaatatgac ttattggaaa cacatctata ctctcccatt
    10201 tattaggtat tctagtttta attacatctc caaccatttc tttctcatat acatcaaaag
    10261 tctttcctgt aagtcccata ttaattgcaa gagttggttt taatcctctt cttatagtct
    10321 taagagatgc cataagacca cgtaaatcaa tagctttact agatatctct ccattttgag
    10381 acttcaattg taaatctaaa aatattcctg caaagtgtat taatttttct tcatcagcat
    10441 ctgaaaattc attttttaaa atcatcatta acttatcttc ttctatagga ggaatatcta
    10501 ttaccataaa tctagatacc agtgcttcat taagttcttt tgttcctgca tattcataat
    10561 tcatagtccc aataaatctt gttgctggat gtagattgac tctctcatac ccaggtacat
    10621 caattattct tctatagtct aaagctgaat gtaccacaac tatagcatca ttttttgcca
    10681 tattaatttc atcaaatacc ccaaaacctc cattaatagc acactcataa actgagcctc
    10741 ttctaagttt aacttcattg tctataaatg tatctgtacc tataagtgtt gaactgtctg
    10801 tatttatatg aaatgatgta ttccactgag gtctattaaa tatttcccct agattatcag
    10861 caagtaagtt ttttcctgtt gcctttggtc ccgataataa tatattttct tcttctaaaa
    10921 tagctgctat acacatagac aagatatctt taccataaaa ataagtttta gactttgtta
    10981 ctctgtattc taaatctttt tctaatccat aataatttct aaaatacagt acatcttcaa
    11041 taagtttttc atctacccct tgcaccctta gattatctat tatatccata tttttacccc
    11101 ttttatataa atagtatttg tttcatttct tatcaccatt ttagcataaa aggatataag
    11161 ctaaaattga ttttttaatc tccccatttt aagtaaaacc atgcaccatt ttatcactac
    11221 aaaattaaga gtactactta ctgatatttc ttgattttgt tcgtctatac gtatataata
    11281 tatgctgata aattatgagg aaaggatgat tgaaatatgg aatatatgtc ttgcccagaa
    11341 gtagcaaaaa aatggggtat ttctgaaaga agagtacaaa aactatgtga agaaagtcgc
    11401 ataccaggag tttcaaaact cgggtatatg tggctgatac caaagaacgc agaaaagcct
    11461 gttgatggta gaacaaaaag aagaaaggaa gtacaatatg aatagcgttt tgattataga
    11521 tgatgacaaa gaactttgtg ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc
    11581 tattactgca cattgtggtg cagaaggtct acatttggtc gataaaaata gacacacact
    11641 ctctcttgta attttggaca taatgatgcc agatatagac ggttttcaag ttctgaaaaa
    11701 aattagagaa acaagtaata ttccagtaat tatgcttact gctaaaaatg acgaggatga
    11761 taaagtttcc ggactacgtt taggtgctga cgattaccta acaaaaccgt tcagcataaa
    11821 cgaacttatg gctcgtgtca attctcttat tcgtcggtat actatgttaa accctgcttt
    11881 tataacagac acagattgca taataataaa tggcatgatt attgacaaga caaaccgaat
    11941 tgtttctgtc aataatattc ctgttcaact tacaagcaaa gaatttgatt tacttgcttt
    12001 tctggcttct aataagggac gggtgtttac caaaaaacag atttatacac aagtatggga
    12061 agaagaatat gcttttgacg atagtaacat catgtctttc atcagtaaat tgagaaaaaa
    12121 gattgaacca gaccccaacc atccatttta tattttgaca gttcgtggtg tcggttatcg
    12181 ttttaataag gaggcttaac atgaatacaa gtgtctatct gcttttgata ttggttctta
    12241 gcctgattat tatagcgtac cttgtttctg tcctgctacg tgtccgaata caacttacac
    12301 ttataaaaga tgctttagag gatataaaaa atggaaacct taatcgacgt attttaacag
    12361 atgaaaacga cattacaaaa caaatttgct atgatattaa cgaaatagca atcaatagcc
    12421 aatttcaact tattcagcaa aaacaatcag aacaagccta caaacagcta atgacaagtc
    12481 tctcccatga tgtaaaaact ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa
    12541 gtaaaattgt tgtaggagag gaaaaagacg agtatatcca tgttgcgtct aataaggctc
    12601 attacttaaa gaattttgta gaaaatctat ttgaatgggt aaaattggat tctaaggaac
    12661 aagtttttca ttttgatatt tttgacttga atgaattatc ccgtaatatt atatctgatt
    12721 ggatacctgt tttagaaagc agtcattttg aatatgaatt tgatatacca gaaatagagt
    12781 acttcttacg tatagacgca aatgcatata cccgtatcat taataaccta ttacaaaata
    12841 ttattaccca tagtagtggc aacaaaatga cattgagaat ttttgaaaac aaagagcaag
    12901 ctcaaattat aataacagat aatgggaaag gtatatcttc agacaatctc ccacatattt
    12961 ttgaaaggtt gtaccaatgc gaccattcac gagcttcaaa gggaaatggt ttaggcttgg
    13021 cgattgcaaa ggaacttatt aacgctcata agggaaatat taccgccaac agcactcccg
    13081 gtatgggaac agaatttaca atattgcttc ctaaagccct gtaaaagtac gaggctttct
    13141 tactctaaaa caagataaaa gcaagattat ggcaaggttt tggcaaggtt catgttttat
    13201 aattctaatt gtagttgatt ttgacgttgt atcaactatc ttttgtagaa acaaatttat
    13261 taaaagtgag gaaattatat atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa
    13321 tttatgggaa tcaaacagta gttgacaaag tcaatctcca tgtaaaaaaa ggccgaatat
    13381 atggtctgtt aggacgcaac ggagctggga aaaccacaat tatgaaaatg attttaggat
    13441 tgacttcaat cacgtatggc gaggtagatg tatttggaga gaatattaag ggacgtgaaa
    13501 aacaagtata tccccgtatc ggtgcaatta tcgaaacgcc aggattttat cctaatctga
    13561 caggaacgga gaacttggag atttttgcaa aactgcgtgg tacagccgcc cctaattctg
    13621 taaaaagagc attggaaatt gtaggcttac cttataaaaa taaaaagctg tttagtaaat
    13681 attcacttgg catgaaacag cgacttggta ttgccaacgc tatcctgcat gacccagaac
    13741 tgttagtttt agatgagcca accaatggtc ttgaccctat tggtattgcg gagatgagaa
    13801 agtttattag ggacttgagc gttgagcatg gaaagacaat tcttatttcc agtcatattc
    13861 tttcagaaat ctcattgttg gctgatgata ttggaattat cgaccacggt gttcttttgg
    13921 aagaaagtag tatgagtgaa ctagaaaaga aaaacagtaa atatatcctg ctacaagttt
    13981 cagatattcc gaagacatca cttattttag agcgtcaatt taacacgaag gattattccg
    14041 ttcaagatga ctacacgtta cacctttata acactaatct ggatatgggt gaaatcaata
    14101 aagcacttgt aatgcagaat gtaattgtta ttagttctca actttgtaat gatactttag
    14161 aggactactt taaaaaaatc acaggaggag agggcattgc ttaaacttat tcaaacagaa
    14221 tttcttaaac tgcgtcgtag gaaacttatt tggctgatgt tgttgtctgc tctcattatg
    14281 ccgtttttcg cactactata cttcaattat tttggtaaaa caggtgttga gccaacaaag
    14341 ttttataaat ggtctgcatt tggttataca ctttggatta ttttgcccgt tgttttgggt
    14401 atattttgca ccatgcttat acacgaagaa aaccagtatg atatgctcaa acagctttgg
    14461 attgtaccta ttagtaaaat gggatacttt tttagtaaat ttttcgttgt tttgatttac
    14521 tctatttgtt ttatgctgat tacagccgtt gcttctgtgt tatttagtgt actttctgga
    14581 tatgtcgtat ttagctgggc aagcatttta tatttgttga aaaaatgttt agaaattggt
    14641 gtaatcactg cttttgtaat gttgccgatt ttggcaatcg ctacatctca aaaagggtac
    14701 attctcccaa tttgtatcac attggtatat gccttttccg gttttgttat aatgacaata
    14761 aatatgtatt tacacccatt atccagtatg ggagttatta tcatgagaaa taaagacatt
    14821 ccaggtctta tattttcaca ggaaattaat gtcccattag catttctttg tatttgtatt
    14881 tggagtattg tatcagtatt gtttgcaaac attgcactag gaagaagaaa gtgaggtgta
    14941 gataatgtgg aaattacttt gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg
    15001 gattacagtt tttgcatcca ttatgatagc agtcatagtt tttgcaggag gtcaggatgt
    15061 atataatgac ccagatttac attatggctt aaagagcgtt tatgacggtt cacggtatat
    15121 agacaatgca ggttggtata tggacgcagt acaaccttgg tcaacttttt ttgttcttcc
    15181 tgctgtcatt gccctattag gaagctatat tatttgtcgt gaagaagagg aggacactat
    15241 taaatccctg cgtcttattc ccataaatga aacaaattta accatagcaa aaatgattat
    15301 tacttttata ttcagtatac ttctttattt gttactcttt actataacat ttttgactga
    15361 atccgtttta catttttccg atttatcgac aaaattggtt ttaagttgca tgaaagagta
    15421 ttttcttgat ggcataggtg tattctttgc aatttctccc attattgcct tagtatcgcg
    15481 catgaaaaaa ggatattggc ttgcattagt attcacagaa gtttattcta ttgctggact
    15541 atttgcgggt atgtcaaatg tcttacaaac cttttttcct attgcggcaa tatttaatat
    15601 ttctggatac tatataacta ctggagaaaa aattatgggg agtatcataa gtttattact
    15661 ttgtgcctgc ctttctgcat ttatactgaa agggctaaaa cacaatgaaa aaaattgaaa
    15721 tatgtatatc aaaatgaaaa agataaaaaa actgattatt ctaattgtat tcttgtttct
    15781 ttttggcatg ttacactgtt atactgaatg gaaattcaac aatgacccac ctaaaggggt
    15841 aatagaaaga ataaatgagt ggactgataa gattggactg gattagtaac ttatgttaat
    15901 tatagtgaat gtaatattag caagttattg tttcgtattt taatactata actttaaata
    15961 tttggtttta atcttccaaa cgaatataaa aaaactgtca cagaggcaac atatttttga
    16021 atatttataa agtggtagtt ttgattttca aagctgccgc cttttcttct atataagcaa
    16081 acttatcctc aacaaagtgt aaaacatggt acattttaag aaaaaagtac cacagacaca
    16141 agcaataaaa gaaacgccga gaacctacaa aaacgtagtg cttatgcaga tttacgacgt
    16201 cttctatttt ctcaaccgtc ttaatgacct tgtacaaaag aaatttttct aatatttttt
    16261 ataatcttat cttgtgatgt tttttatgta taaatgactt aataacaaat ccaataaaaa
    16321 ttatggatat tggtataatt agaacgagga atttataatc attataatgt gacattattc
    16381 ttacccaact ctcagaaaaa atatatccta tactgattaa tgaagtattc catatggtta
    16441 ttcccacgat agatgatatt acaaaagtta taatattttg acctgctata cctgctataa
    16501 aagatatgta tgttctacat aaagggatta atctacatac acaaacagaa atagaagagt
    16561 atttattaaa ataatcttga gatgcaaata ttcctttctt tgcctttgga catatctcta
    16621 ttattttatt tatgatagat ttacctcccc aatttcccac tgtataacaa aatatcgaac
    16681 caagtatacc tgcaattata cttaaaatta aaattgtcag aaatccaaaa tgatttcttg
    16741 cagcaattgc tccacataaa ggtaaaacaa cttcgctagg taatggaaaa caagcatact
    16801 caatcataat taatacaaat acagagatta atccatattg taaaataaaa ctattgatta
    16861 cagacactta cttatctatc tcctttctta tttaaaggtt ttaagggtac atgttctaag
    16921 gattctcacc ctatatttaa agaatcaatc tctttaaact attatatgta tttacacaaa
    16981 acatttataa catactttaa aaatagtacc ttaaaataag aaacttaata aataaaaaaa
    17041 gattacgtgg ctacgtccta ctctcccagg cagtttccca ccaagtacca tcagcgctag
    17101 agagcttaac ttctgtgttc ggaatgggaa caggtgtatc ctctctgcta ttgtaaccac
    17161 ataatcttta tgcttaatat ttctattaag tttttaataa gtttatagaa cctagtaata
    17221 ctagtttctt tagagttagc actctaaaaa ct
//
LOCUS       DAFKMX010000083        17121 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFKMX010000083 DAFKMX010000000
VERSION     DAFKMX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.083"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            95..2164
                     /locus_tag="LZB08_002107"
                     /pseudo
     CDS             95..2164
                     /locus_tag="LZB08_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2161..3447
                     /locus_tag="LZB08_002108"
     CDS             2161..3447
                     /locus_tag="LZB08_002108"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBM7315609.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     DLLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDNNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDIHR"
     gene            3515..4027
                     /locus_tag="LZB08_002109"
     CDS             3515..4027
                     /locus_tag="LZB08_002109"
                     /inference="COORDINATES: protein motif:HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBM7315610.1"
                     /translation="MINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKD
                     YFDDLVDKFKKLVWIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLE
                     DSAFWNFKNQPDEDRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFD
                     NTEIVKKIEY"
     gene            4343..4966
                     /locus_tag="LZB08_002110"
     CDS             4343..4966
                     /locus_tag="LZB08_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315611.1"
                     /translation="MKMDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRK
                     AEILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENT
                     LKITANKNSNSGVISYIKIPKNEVGTSIVYCKHNYQSLSEFHMESSMSANLNINVIKL
                     GIVKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            4991..5479
                     /locus_tag="LZB08_002111"
     CDS             4991..5479
                     /locus_tag="LZB08_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBM7315612.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DK"
     gene            5592..5753
                     /locus_tag="LZB08_002112"
     CDS             5592..5753
                     /locus_tag="LZB08_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315613.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            5993..6220
                     /locus_tag="LZB08_002113"
     CDS             5993..6220
                     /locus_tag="LZB08_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315614.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6217..9156
                     /locus_tag="LZB08_002114"
     CDS             6217..9156
                     /locus_tag="LZB08_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBM7315615.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVYGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9156..9830
                     /locus_tag="LZB08_002115"
     CDS             9156..9830
                     /locus_tag="LZB08_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315616.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9991..10407
                     /locus_tag="LZB08_002116"
                     /pseudo
     CDS             9991..10407
                     /locus_tag="LZB08_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10367..12814
                     /locus_tag="LZB08_002117"
                     /pseudo
     CDS             10367..12814
                     /locus_tag="LZB08_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12954..13376
                     /locus_tag="LZB08_002118"
     CDS             12954..13376
                     /locus_tag="LZB08_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315617.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13914..14948
                     /locus_tag="LZB08_002119"
     CDS             13914..14948
                     /locus_tag="LZB08_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBM7315618.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGITGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     TYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15035..15238
                     /locus_tag="LZB08_002120"
     CDS             15035..15238
                     /locus_tag="LZB08_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBM7315619.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15409..16569
                     /locus_tag="LZB08_002121"
     CDS             15409..16569
                     /locus_tag="LZB08_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBM7315620.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDAIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTNLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16829..16969
                     /locus_tag="LZB08_002122"
     CDS             16829..16969
                     /locus_tag="LZB08_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315621.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7002 a         1666 c         2983 g         5470 t
ORIGIN      
        1 tagaaatatt ttagcataag atgtataata aaggaaaaaa tacagaatta aaaaacataa
       61 gatagataat tggacttagg ggagagaaat tattatgtaa ttgacaaaaa taattcttag
      121 taactatagg tgttttggag aagaacctac aactattgaa attaataatt taactggatt
      181 tataggtcat aatagcagtg gtaaaactgc tttattaagt gcacttatga aactttttgg
      241 agaaaagtct agtgatagaa ttattgaaag atcagatttc catatacctg aaagtattga
      301 tccagatagt atttctaaga atgaattata tattgaagca caatttgagt ttcctgaatt
      361 ggagagaggt aaagacagca tatctatacc agtatttttt aaaaatatgg ttgttagtga
      421 accaggtggg aaaccttatt taaggattag acttgaagca aattgggaga aaagctcaaa
      481 tcctgaagga agtattgatt caaatgtata ctatataaca tctacaacag aggagataaa
      541 tcctgaagat aagcgtaata tcaaaagata tgagcttgat caaataaaga tgatttatgt
      601 acctgccatt aggagaccag atgaacagtt aaaaaatgtc tcaggaacaa taatttcaag
      661 attattaaat ggtataagtt ggtcagaaca aacaaaggca agaattaaaa cgaaagcaag
      721 tgaaacggag gaaattttct ttgaagaaaa aggggttgaa tcattaaaga aatcactcga
      781 aactcaatgg aatagttatc ataaggatat tagatattct aatccaaaga taaacttcaa
      841 tagtactgat ttagagacaa ttttaaaaaa agttgaagta actttttcac ctacagggac
      901 acatcgagat catcgggttg aagagcttgg agatgggtta cgttctttat tttatctttc
      961 acttgtaaat acatttttag aaattgaaga agaaattcta aaatctatta atgatggtaa
     1021 agaagaagat atatttacat taataccgcc tgtattaaca attgttgcgg ttgaagaacc
     1081 agaaaatcat atttcacctc atcttttagg aagggtagtt gaaaaattaa attttatttc
     1141 tacaaaagaa aattcacaga caatattgac atcacattca ccagcaatag taaaaagaat
     1201 tgaaccaaca gacataaggt attttaggat ttgtaaggaa tatgaatgta caaaagtgaa
     1261 gtcaattata ttaccaaatg aagaaaagga agcagaacaa tttaaatttg taaaggaatc
     1321 agtattagca tatcctgaac tttattttgc aaaacttgtt gtattgggcg aaggtgatag
     1381 tgaagagatc attattaagc agatgattga attaaaagca gaatctgtag attcatcaga
     1441 aatttcaata gtaccattag gtggtaggca tgttaatcat ttttggcgtt tattaaatga
     1501 tttagaaatt ccatttatta ctttgttaga tttagataga gaacgagatg gaggtggttg
     1561 gggacgtata aagtatgcaa tacaacaatt aataaatatt ggtcatgata agaatgtatt
     1621 actttataca tgtgaaggaa tttcacaaga taatgaattt aaagagatgc atacttggga
     1681 tgtaactgat actgaaaata tggctaaatg gattgaaatg ctaaaaggtt atgatgttta
     1741 tttttcagct ccgttagata tagattttgc aatgttacaa agttttaaag ataaatatat
     1801 ttcagcatta acaagtgcgg aaggtcctag gataaaagat catggaaaaa tacaagatat
     1861 tgtgatcaat gaagatgctg aagatgaatc attgaaagct gcttataaag atagaagaga
     1921 tagtgatatt aaatcaacct taaaacaaga aggtggcgat ggatctactt atactgaggg
     1981 agagaaagaa ctaatgatat ggtattcata tttcttttta ggaagaggta aaccaactac
     2041 tcatttatct ttattttcta ataatactga tatagatttt gaaagttttc catactgtct
     2101 aaaggagttt gtaaatagta ttattgaaaa gatgatagat gattcagggg gtggaagaaa
     2161 atgaagagga attgggttag taaagaagat tggagtccag cggctggatt cagactagaa
     2221 gttgaagcga tgaatacagt tattaatgat aagaatacac ttgttgtagc tggtcctggc
     2281 gctggaaaaa cagatttatt agctcagaga gcctgctttt tactagagac taatacctgt
     2341 agatatccta aaaaaatcct tgctattagt tttaaaaagg atgctgctga aaatttaaaa
     2401 gagcgtttag aattgagatg tgggaaagaa ttagcttcaa gatttgaatc aaagacctat
     2461 gatgcttttg caaaagaaat cgtagataga tttagaaata gtttagatga agcatataga
     2521 cctttaaata attacaatat agctgaaaat cgtgatgtta gaagagcatt tgagatagca
     2581 ggattaaatt tcagaggaaa tcaaactgaa tttaataaag tatatggaaa taaactatcg
     2641 ataaataaat tgcctttaca tacagatttt gtagataaga tttttataga tgcttggaat
     2701 gtactactta aaggcaacaa agataataat tttgtaagtt cacttacttt tggtatgata
     2761 tcacgtttag ccgaatattt gataagaacc aataggtata ttaagaaagc aattgaaatg
     2821 acttatagtc atgtgttttt agatgagttt caagatacta aatctattca atatgatctt
     2881 gttaaagctt gtttttttaa aacagaaaca ataattactg ctgtagggga tggcaaacaa
     2941 cgaattatgt tatgggctgg tgctagaaag tcaatatttg aagattttca aaatgatttt
     3001 ttaagtcaaa aaagaacatt aattatgaat catcgttcag cgccaagact tgtagagatt
     3061 caaaaagcaa tgtataacag tttgaatgaa gaggcaattg atttaaaaac taatgaaaag
     3121 tggaattatg aagatggaga agtgtattta agactttttg aggattatat aaaagaagct
     3181 gatatactta gtgaggaaat agtcgaattg gtggaaacag gtatagaaat taatgaaata
     3241 tgtatattag taaaacagag cgtagatatt tattcaaaag agattataaa taaactttct
     3301 gaacgtaata ttaaggcgcg taatgaagca atgtatcagg agatattaaa agaagatata
     3361 ataaagacac tggtttcgct ttttaggttg tcgttaagtg atagaaatcc cgatgattgg
     3421 gattatattt acgatattca taggtgaatt atatggaata gacgaagggt ataaccccaa
     3481 aatattaaat gatttcatta ccaaagtaga attaatgatt aatgatctca aggtaaattt
     3541 aaatattact gatgaaaaac aattttcaga tttagttgat tctaaggttg aatatatttc
     3601 atatgaaaaa tttgcatcaa agtttcaaaa atataaatct aaagattatt ttgatgattt
     3661 ggtagataaa tttaaaaagc tggtatggat tgaatataat gaaactaaag attggattaa
     3721 agcgattgat aactttgaag gtaaaaattc aattccaata atgacaattc ataaaagtaa
     3781 aggacttgaa tatgatacta tcttttttgt tgggttagaa gattcagcat tttggaattt
     3841 taaaaatcaa ccagatgaag atagatgtgc attttttgtt gcgttatcaa gagctaaaag
     3901 aagaattgat tttacattta gcttagatag accagcagga ttttcgaatt cacagaagca
     3961 ttattgtatt aatgaattct acgaattgtt tgataatact gagattgtaa aaaagataga
     4021 gtattgatta agatgataaa aactttagta tattatattt taagatatta gttatataag
     4081 tagacattga atttatgtat ttgatgtcta ttttttatat aaaaatatat taaagaaagg
     4141 atgaatttgc atgaagaaaa aaacaaaaat atctaaaaag attgtaagtt ctattatggt
     4201 ttttataact atagttacta ctatacttac atcatcagtt tcagcttatg ctagcgagtt
     4261 ggatatgagt gaacagacag gctttaatta tttaggtatt agtccaatta caggaagaaa
     4321 aattaatcat aacatttatc gtatgaagat ggataataaa attgtatttt gtgtagagcc
     4381 aggaattaaa acttcaagtg gaggaggata tatatcggag gagttaggag ataaatttat
     4441 atctacaaca cttccaaatt atcataaaag aaaagctgaa atacttgaga aggtaaatag
     4501 acataatata ttaccttcat ggaataatga aaaagttgca gttaaaagtg gagaaacaat
     4561 tgagttagaa gataagaatg gtattattag agaaatgcaa ttagaatcaa ataatactgg
     4621 aacagatata ttacttgaag aaaatacttt gaaaattaca gcaaataaaa attctaattc
     4681 aggagttatt agttatataa agattcctaa aaatgaggtt ggaacatcaa tagtttattg
     4741 taagcataac tatcaaagct tatcagaatt tcatatggaa agttcgatga gtgcaaattt
     4801 aaatataaat gtaatcaagt taggaattgt taaagtaaaa aagattgatg aaggcacagg
     4861 aaaggaatta gcaaatgcaa aacttagatt tgagtatgat ggtgatagta aagaaattat
     4921 aacgggttct aatgaaattg ctagtataga agatattatt ggttgattaa aaagtgaagt
     4981 atacttttgt atctatgaaa aagactataa gcaatatata aaattagata tacctattga
     5041 tgaagcaaaa ataaaaaata gatttgaaat aaggttaaaa aatgaaagag catatcatgc
     5101 agcagttgat ttgttagcaa atagagatga agaaaagaca gcattttcaa ttattaatcg
     5161 ttatatacgt tttgttgata aagatgatga aaaaattaga agtgaatgga aaactaatga
     5221 tcagtggagt tattttattg gtgaaggtag agcgaagtta aaactaacaa caaatccaga
     5281 accatataat attcaaaaaa ctttaaattg gttatcaaga caagttgcac caacgttaaa
     5341 aacaattaaa aggttagata gtttgaacaa ctcaacatta attgagggct taataaggta
     5401 tgcaagacta acagataaac ataaaaaaat aattgaacaa aattcagtaa atgttgaaga
     5461 cattatttat gataaataaa aaaatgtaat tagtattgca gattttaata taaaagaagg
     5521 gagatatacg aatgaatttt ggtcaaaatt tatataatta gtttttatca aatgcacaat
     5581 cattggtatt aatggctata gctataattg gtgtatattt aggatttaag agagagtttt
     5641 caaaacttat aggattttta gtagttgcat taattgcagt agggcttgtg tttaatacag
     5701 caggagttaa agatatatta ttggagctat ttaataaaat aataggtgtg taaggattaa
     5761 aagagaatag aaaattatga gtatatgatg aagggttcta ataagtttta ttaaaatata
     5821 tattgaaaga caagtagagc tatttcgagt tattacatgt aatgctaata tatataactg
     5881 cattttggaa attaaataca taattagaaa tagcaaattt tataggtgac ttattaatat
     5941 gtttacttat tgtatactat ttgatatatt taatatgagg tgattcttga aaatggacat
     6001 agtgaaagtt tttgctaaca atgtgagaaa atatagaaat aaaaaaggtc tatcacaaga
     6061 agcttttgct gaaaaagctg gattgcatag aacttatatt tgtgctattg aaagaaaaaa
     6121 gagaagtatt gctttagata atgtgcagaa aattgctgat gcgcttgaga ttgagactta
     6181 cttactattt atagaaggtg aggagaaaga atgtttatga aaaaattacc aatagaaaag
     6241 cttatagaaa atttaggttt ttcagggtca aagaatcttg tgaatataga tatgcttaca
     6301 aaattagata tatctaaaca tgatgtaaaa actttaaaag ttataaatcc ttatgcaatt
     6361 tatttagtaa atagtaagcc atttatatta tttatagagt cttcttttga agaaaaaaaa
     6421 ttacaacaaa ttagtaagct tatatggaat gctcaaatac caattgcttt tatatgcgat
     6481 tctaattctg ttaaaatatt taatggaaaa gctttggatt taaagtcata tttaattgag
     6541 gaaatatata atcaatctat tgaggatttt tcggaagaat ctgagttttc ttatttgaaa
     6601 atatctgatc cattattttg ggatcaatac actaaacaat ataccaaaac gcatttaaat
     6661 gattatttgc tagataatat tacttcattg acaaataaat taaagaaaaa atataaggtg
     6721 aaatttgcaa cgaagcttgt tctaagatta atatttatta gatatttaat cgatagaggt
     6781 gtcgatatag cttataaaaa ttttaatcat gatattaaag cttcacaaaa agaactgcta
     6841 aagattacta aaaacaaagt tgaattatat gaactgttta aatacttaaa agaaaagttt
     6901 aatggtaatt tatttgagtt aaatgacgaa attaataata cttgtttaac agatgaagtg
     6961 tttattttac tatctgattt tttatcaggt aaaataagta ttgacaatgg acaaatgtct
     7021 ttattttcaa tgtatgactt taatatcata ccagttgaac taattagtaa tatatacgaa
     7081 attcttttag gaaaagaaat tcgtaataaa gataacgcat tctatactcc gaattactta
     7141 gcagaatata ttttagataa aacaacactt gaatttctga aaaataatag gcaatacaaa
     7201 attctagatc cttcttgtgg atcaggagta tttttagtaa atagttttag acgtattatt
     7261 gatatgaatt tgggtgataa acttttttgt gaagatgata aacttttaaa agaactttta
     7321 ttatctaata tttatggaat tgatatcaat gaagatgcta tcgatgttac tattttctcg
     7381 ttatatctaa ctattttaga ttacaaagac cctaagatat tatcagaatt taaactgcct
     7441 aatttaaaag ggtgtaattt aatagcgagt gatttttttg atgataataa attggagagt
     7501 ttaaagaaaa ttaactttga ttttatcatt ggaaatccgc cttggggaag tgtctcagat
     7561 ggattacatt tagattattg caataaaaat ggctataatg atatgcaaca aaacaaagaa
     7621 atcagtagaa gttttatttt tagagcaaaa gattttagct ctagaaatac agtatgttct
     7681 tttgtagtgc attctaaaat actatatact caaaaaaagc catcaaaaaa attccgtgaa
     7741 tttttgctta aaaaaagtca aatatttagt gtagttgaaa tgtcatctgt aagaaaatta
     7801 gtgtttaaaa atgcagacgc tcctgcagca gttatatcat ttaagtataa cgaaagtgaa
     7861 gaaaatttaa ataatagaat catctataca tctttaaaac caaatgtatt ttttaaatta
     7921 tttaacatta ttgtaattga aaaatatgat gttaaatata tttcacaaaa actattattg
     7981 gagaatgatt gggcttggaa aactattgtg tatggattca gtaaagatat agatataatt
     8041 atgaatatca aaaaaaaata tcaaactgta aatgaaatta ttttgaaaaa cagttttgtt
     8101 tatggaacag ggatacaaat caatggtaat gaaagccatg atgcgacaca tttaaaagga
     8161 aagtggtatg ttgattcaaa gaaaggaata gattcattct atgttgatat aaataaaggg
     8221 aaagaactta ctcaagataa aatgagaaga cctaaggcta atcagcaaga aatatttatt
     8281 cctccatatg tattattaaa aaagggagtt gacacgaaaa catatagata tagagcagca
     8341 ttttcaaatg atgcatttgt atattcagat gcagttacag gaatatgtgg aaaaaaagat
     8401 aatttaacta cactttattg cttgactggt atgcttaact cttcttttta ctcatactta
     8461 aatataatgc taggtacatc aattggaata gaaagagaac aaggattttc aacggaaatt
     8521 tttacgtatc cagcaataga aagtgatgaa attgctaatc tggtagtaaa aatacaagaa
     8581 gcgactaaat tagaaaaaac aactatgttc ttgaatgaaa aaaatagttg taagtatatc
     8641 aaacaattag ataagttaat tttgaagtct tttaatttaa atgatgataa atttattgac
     8701 tatgcaataa atattcaaat tcctgagctt acaaattcta agtcaactaa catatataga
     8761 aaaataacta aggaagattt aattaattat agtgaatgtt tcaaagaaca gttttcatcg
     8821 atatatcgta gggttgggaa aagtatttca atttcggtat atccaaaaat attaaatcgt
     8881 tttacagtat ttgaactaga agtaacagat gaaaaaacag atagagagat tatggttaaa
     8941 gcagaagctg ataataatat ggagttgttt acgaaaataa ttagatatga ttataatgat
     9001 gtttttcatc aaattagaga tattattcat tttggagaaa attcatttta tatcataaag
     9061 cctaattgct ataaatattg gcatcctgct atcgcagaaa tggatcttgc tgatgttttg
     9121 gatcagattt tatctgatac agggggtgaa gaataatggg aggatttaaa aattcttcac
     9181 taaactttta tttaaagcaa gggctttatg aaataattat agatggatta atagactgtt
     9241 gtttacttat gagcaaatca tgtattgaac aatctagaaa aatattaaat cacgaagaaa
     9301 agataagaac gcatttagtt gaaaattatt tagaaaatga tattgaaaga agaaaaacat
     9361 ctttatctaa acaacatcta agatttgaag tagaagcaca agaaaattat gacaacaaaa
     9421 atgaatctta tgagggtagg gttgatataa aagttatatc taaagacact ttagcggata
     9481 gaagggctta ttatatcatt gaatgtaaaa ggatagatgg taaggaaact ttaaataaaa
     9541 aatatataac cgaaggtgta caacgattta ttactgagaa atattcttcg cattatggca
     9601 aaaatataat gcttggattt atagtaaaag atataaatat ccagagcaat atacaaaaaa
     9661 ttaattcact gaatcagaac atattatata catatataga tacagatatg ttgacagtta
     9721 gaagtgattc tgaaaataat tatgctgttt gtgaaagtaa atataaagta aaatctaaac
     9781 tactattgtt aaatcatatt ttttataact tttcgagtat aataagataa tcttatgaat
     9841 ataaaatatt actatgttaa aattatagtt tggttttgat aatattataa aaataagttt
     9901 aagaataaaa tataaaggaa aaaaggggcg tagcactatt ttttagtgtg acagcccctt
     9961 taattttaaa atcggtaagg aggagtgtat ttgaaaaaaa ttaagtcata tacagggatt
    10021 tggaatgtgg agaaggttat atatgcaata aatgattttc agcttccatt tccagttact
    10081 tttacgcaga tgacatggtt tgtattaaca ctttttataa ttatattatt tggtaagtta
    10141 cctccattat ttatgataga aggagcattt ttaaagtatc ttggcatacc aatttttata
    10201 acttagttta tgtctcaaaa gacttttgat ggaaagaaac catatggatt tttaaagtca
    10261 ataatactat attcattaag acctaaaata acatacgcag gaaaaaacat taaatttaaa
    10321 aataaaatta ttgatgaaaa tataactata gtaaggagtg aattatatgt tcccgataaa
    10381 atatatagag aacaatcttg tatttaataa ggaaggagaa tgttttgcat attatgaatt
    10441 aataccatat aattattcat tcttataagc tgaacaaaaa tttattgtac atgataattt
    10501 taggcaatta atatcacaaa gtagaaatgg taaaattcat gcacttcaaa tagccactga
    10561 aagttctata agagttacac aagaaaggtt aaagaaaaat attacaggaa aattaaaaga
    10621 aattgcttat gaaaaaatag acgagcaaac agaggcactt gtttctatga ttggcgataa
    10681 tcaaattgat tatcattttt ttattgggtt taagctttta gttacagaga aagaaatgaa
    10741 tataaagagt ataaaaaagt caatattagc taatctatca gattttatat atgatgttaa
    10801 taataaattg atggatgact ttgtcagtat aaataatgat gaaattatga gatttttaaa
    10861 aatggaaaag ttattagaaa ataaaatctc aagaagattt aaagttagaa ggttagataa
    10921 aaatgatttt ggatatttaa ttgaacataa ttatggaaag aaaggcacag tttttgaaga
    10981 ttatcaatac catttaccta caaagaaact taaaagagaa acattagtaa agaaatacaa
    11041 tcttataaag ccaactagat gtcttataga agagaagcaa agatacttaa aaattataca
    11101 tgaggattca gaaagttttg tagcttattt tactattgaa attattattg gagaaatgga
    11161 ttttccatct agtgagatat tctattatca gcaacaacag tttgcatttc ctattgatac
    11221 tagtatgaat gtggaaattg taacaaataa aaaggcttta actacagtta gaaataagaa
    11281 aaaagaatta aaagatttag acaatcatgc ttggcagtca gggaatgaaa cagtaacaaa
    11341 tgtagtagaa gcattagagg ctgtaaatga gcttgtaaat aatttaaatc aaacaaaaga
    11401 atcaatgtat aagttaagct atgtaattag ggtatcagca gatagcattg atgaattaaa
    11461 acgccgttgt gatgaagtta aagattttta tgacgattta aatataaaat tagtacgcct
    11521 aatgggagat atgataggat ttcacaatga atttatacct tcaagcaaaa ggtatataaa
    11581 tgattatatt caatatgtaa ctagtgattt tctagcagga ttagggtttg gagcaactca
    11641 atagcttgga gagaacgaag gaatatatat tggatatagt ttagatacag gaagaaatgt
    11701 ctatcttcaa ccatctcttg caagtcaagg agtaaaagaa tctgtaacta atgcgttggt
    11761 agcagcattc tttggatttt taggaggtgg gaaatcattt gctaataact tacttgttta
    11821 ctattcagta ttgtttggag ggcaagctgt tttggtggat ccaaaagctg aacgtggcaa
    11881 atggaaagaa aatttaccag atatatatca tgaagtaaat attgttaatt taacaagtga
    11941 tatagaaaat aagggaatgc tagatcctta tgtaattatg aaagaagtaa aagatagtgg
    12001 aagtctagct attgatatat taacttttct tacaggaata agtagtcatg atggagagca
    12061 gtttcctata cttagaaaag caatcataaa tgttactcaa gacaaatgca gaggactttt
    12121 gaaggtaata gatgaattaa ggaaagataa ttctcaaata tcaaataata ttgcagatca
    12181 tattgaaagt tttacagatt atgattttgc acaattactt ttctcagatg gaaatattaa
    12241 aggctcaatt aatctagaaa aacaaatgaa tataatacaa gttgcagatc ttgttttacc
    12301 gaataaagat actttatttg aagaatatac aacaatggaa agtctttcag tagctatgtt
    12361 gatagtaatt agtacttttg cattagattt tatccataca gatagaagta cttttaaaat
    12421 agtagattta gatgaagctt ggacatttct taatgtagca caaggaaaaa ctttatctaa
    12481 taaattagtt agagcaggat gtgctatgaa tgcaggagtg tattttgtaa ctcaaaatgc
    12541 aggtgatgta tcagatgaaa atttaaaaaa caatattgga ttaaaatttg cattcagaag
    12601 cacagatatc aatgaaatta agagaatact tgaatttttt ggagttgata aggaagatga
    12661 gaataatcaa aaaaggttaa gagagttaga gaatggacag tgtttaatac aagatttata
    12721 tggacgagtt agggtaatac aaatacatct agtttttcaa gaactcctta ctgcatttgg
    12781 tacaagacca cctataagaa aagaggtgga ttaatgaaat cgaaagtatt tagaaattta
    12841 agctggataa ctattttaat agtagtactt ttactatttt ttatttatat aaatttatag
    12901 atttagagaa acaaatagat acattagata agaaaagaaa aaaactcata gatatgagat
    12961 tagaagatat aattgataaa gaatcatatg aagaaaagta tgatgattta acagttgaaa
    13021 taaagaaact tagtaaagag aaaaaaactc ttaaagcatc attaataaat gaaaagagta
    13081 taaaagcaag acttagagaa tttagaaaga tacttgaaag aaatgaattg ttagaaaaat
    13141 ttgatagata tgtatttgca agtatagtag ataaggttat agttggagtt attaatgaag
    13201 atggtatagt tgatccttat aaaataactt ttgtttataa aactagcttt gatgataaac
    13261 aagatggaca taaatttata aataagagtc cgttagaaac gtattcaaaa gataacaacg
    13321 ataataaaaa gaagtgttca tatactaata atgagacatt gcgagactgt agttaaactt
    13381 attagagaat aacaaggttt cagactttta tattgtgtct taaaagtaga tttgccacct
    13441 atttgccacc ataattattt ttgggtggca aatttattct acaatgattt caaaaatatt
    13501 taccgtctgg tttctcatgt tattagttac atgtaaatat gtatccatag ttgttgaagt
    13561 tttagcatgt ccgagtatat gctggatatc ttttatatta gtaccatttt ctaataacat
    13621 agtatcaggg tttttaaaat aaagtgtgta ttcttctaaa gtagtgataa ttaaaatatc
    13681 aaatatcact atacaaagga aagtgcacgc tttttatgtt taacaaaaac gaaatattaa
    13741 aaaacttatt gaagaatatg atgtcaaaac aactacagat attcaagata tgctaaaaga
    13801 tttatttaca aatacaattc atcaaatatt tacattgtcg aattttaacg gtttaaacat
    13861 aatatattga ctgatataat aaaatcatag aatattttag ggggaaatag attatgttta
    13921 agaaaaagtt aatagctgtt gcaatgagta taactatgat tagtgtaggg agcttttcgt
    13981 atgctcattc aggaagaact gattcatcag gaggtcatcg tgataataaa aataaatctg
    14041 gattaggaag ttatcactat cattgtggtg gttatccagc tcatttacat aataatggtg
    14101 gttgtcctta tactggagga ggaagcagtt ctggaacaac aacttcggtt aataatgaag
    14161 aaaagcaaaa gaggtctgtt ggagaaaaag gatataatca agggtatgaa gatgggtata
    14221 aaggaaatta tagttcatca aattatagtg gagattatag tgatacatat gaatcaaagt
    14281 actcagaagg atatgaaaaa ggtaaggcta aattagaaga ggaagaaaag gtggctaaag
    14341 agactggata taatttaggg attacaggtg ctaaatctaa taacacatat gaaaaagaag
    14401 ttttaaaaaa tgcttatgat accggatatt caactggata caatgaatat aagactaaaa
    14461 agatagaaga atataaagct aaagggatag aggatagcaa taaagataaa gaaaaaatga
    14521 catttgaaga aaacatagat tctgaattta tagatgctta taataatacc tatgacgaaa
    14581 tacaagaaca attaaaaaat gattacacta ctcaaggatt tgagagtgct atcaaaggag
    14641 aaagatttga cacatcaact ataggtaatg ttaaatatgc taattggttt aaagaaggat
    14701 atgataatgg taaggttaag ttacctaaag caaaagaaag tgtatataat caaggttaca
    14761 atgaagaaga ttttagtata ccagatgaaa tgaagtctat tgaaactaag ttaaaaggtg
    14821 tatatgatga aggtttagaa aaaagagaag aagagaaaag tagaaatgct acttatgggg
    14881 ttggagctgg aacagcagct gtagttgctg gagtagtata taagaagaaa aaacgtaaaa
    14941 agatatagat gctagatata ccaatgatga caagtgtata acaaatataa ttttaagtat
    15001 aaatatttaa aatatagaag ggaaggttat atctatggga tttaacttta ggaaaagcat
    15061 aaatctagga ggaggactca aattaaattt aggtaagaag agtgtaggta taagtgctgg
    15121 tgttaaaggt gctagagtgt cagttaattc aaaaggaaga aaatcagcta cattgagtat
    15181 acctggaact ggaatatcat atacaaaaac tagtacaaag aaacgtaaac ctaaataaat
    15241 atttgatata ctaagaaata gactgttaca aatagtctat tttttagtat atagatttaa
    15301 aaagtgacaa aattttgaaa tataaactaa tttcataaag gaaaatattc ttttatgtag
    15361 aattataata tttaagtaat attataaaat ttataggagg atttcattat gaaattaagc
    15421 aaaacactat tggcaatagg gatgtctgtt tcattattga ttgctagttg tccaacatca
    15481 aacgctctta gttctattaa ttcaataaaa ggtacaaata agtatgaaac agcaggttta
    15541 atagcagata aacaaaatta tgatattgca atacttatta attctgaaaa tggattatct
    15601 gatggattgt cagcaagtgg attgtcagga gctaaaaatg ccccaatatt gttaactaag
    15661 aaagatgcaa tcccttcaga aacattaaaa agattagaaa aggtaaaaga agtatatcta
    15721 attggaggaa aaaattcgat aggagcatca gttcagaaaa tattaagtga taagaaaata
    15781 agcataaata gaataagtgg aactgataga atagatacaa gtcataaagt tgcaaaagaa
    15841 ataggaagag taagtacggt taaaaaagta atattaacta atgcttttaa aggcgaacca
    15901 gatgctatga gtgttgcttc tatagcagtt agagataaat ctccaatagt attaactgat
    15961 ggaaatacta caagttttgc tacagatgga ttagaaactt atgcaattgg tggaaaagtc
    16021 agtatgagtg ataagttaat aaatgaaact aaagcaacaa gaataggtgg agtagataga
    16081 tacgatacaa ataaacaaat aataaataaa ttttataatg gctcaaaaga attttacata
    16141 gcaagtggga ctaatttagt ttattcttta gttggttcta caattgcaca aaaaacacct
    16201 atagtgttag ttggaaatgg aagtaataag agtatattaa aaaatgcaag taaaataact
    16261 gctattggaa atattgatga tgttgttata aaacaatgtt taaatataac taataatgtt
    16321 ggagattcaa atacaggtat tgtaaaagat actaattcaa ataatacgaa caaaccaagt
    16381 aatgagccta gctctaataa tcctggatta actgataaaa gtacagtata tgtaactcct
    16441 aatggaaaat catatcatct aacaaaaaat tgttctacac taaaaagaag taaaacaata
    16501 aataaagtaa cattaaaaca agctaagtca caaggtaaaa tagacccttg taatgtttgt
    16561 gtaaaataat gtattagcca aatatatatt aaaaaagaca attaattaag attgtctttt
    16621 ttattgaaga aatataaatt tatattatgt attattaaaa agaaaattat taattataaa
    16681 aaataataat aaagacgata agtattaaaa ttttatgttt ccaatattta tgatataatt
    16741 aatatggcaa ggaataaata tttgaaaaag ctatgagtgg tgtttccatg gaagtttctt
    16801 cactttctta tgagaggagg tgaaaactat ggaaaatcta atgatgagta ttataactgg
    16861 tgttatagcc agttacattt acgataaatt aaagagccac tctgagcgac caactaagag
    16921 tggctgggaa cttaatataa agttccagaa aagtaaatct tcaaaataat ttgttgaagg
    16981 aaacatcact caaagttaag taaaatatgt ttccttgctt ttattatatc acaaattaaa
    17041 aatattaaca caaaattttg tgaatatata cttattattg ttctaaaaaa atatgtaaat
    17101 aattgatttg attgcatgga a
//
LOCUS       DAFKMX010000084        17092 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFKMX010000084 DAFKMX010000000
VERSION     DAFKMX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.084"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            230..1531
                     /locus_tag="LZB08_002123"
     CDS             230..1531
                     /locus_tag="LZB08_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7315622.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1607..1882
                     /locus_tag="LZB08_002124"
     CDS             1607..1882
                     /locus_tag="LZB08_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBM7315623.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3249..3479
                     /locus_tag="LZB08_002125"
     CDS             3249..3479
                     /locus_tag="LZB08_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7315624.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3472..4053
                     /locus_tag="LZB08_002126"
     CDS             3472..4053
                     /locus_tag="LZB08_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7315625.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4633..5310)
                     /locus_tag="LZB08_002127"
     CDS             complement(4633..5310)
                     /locus_tag="LZB08_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBM7315626.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5499..6863)
                     /locus_tag="LZB08_002128"
     CDS             complement(5499..6863)
                     /locus_tag="LZB08_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7315627.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6991..7512
                     /locus_tag="LZB08_002129"
     CDS             6991..7512
                     /locus_tag="LZB08_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBM7315628.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            7644..7787
                     /locus_tag="LZB08_002130"
     CDS             7644..7787
                     /locus_tag="LZB08_002130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315629.1"
                     /translation="MLNFLRARARARARARARARARARARARARARARARARARARAI
                     SKV"
     gene            8005..9084
                     /locus_tag="LZB08_002131"
     CDS             8005..9084
                     /locus_tag="LZB08_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBM7315630.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDNINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            9408..9776
                     /locus_tag="LZB08_002132"
     CDS             9408..9776
                     /locus_tag="LZB08_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBM7315631.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            9893..10690
                     /locus_tag="LZB08_002133"
     CDS             9893..10690
                     /locus_tag="LZB08_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7315632.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVREYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            11082..11972
                     /locus_tag="LZB08_002134"
     CDS             11082..11972
                     /locus_tag="LZB08_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7315633.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
     gene            12586..13029
                     /locus_tag="LZB08_002135"
     CDS             12586..13029
                     /locus_tag="LZB08_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7315634.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            13201..13371
                     /locus_tag="LZB08_002136"
     CDS             13201..13371
                     /locus_tag="LZB08_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315635.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            13744..14865
                     /locus_tag="LZB08_002137"
     CDS             13744..14865
                     /locus_tag="LZB08_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBM7315636.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            15070..15558
                     /locus_tag="LZB08_002138"
     CDS             15070..15558
                     /locus_tag="LZB08_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBM7315637.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            15644..16051
                     /locus_tag="LZB08_002139"
     CDS             15644..16051
                     /locus_tag="LZB08_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315638.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            16052..16270
                     /locus_tag="LZB08_002140"
     CDS             16052..16270
                     /locus_tag="LZB08_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315639.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            16827..17092
                     /locus_tag="LZB08_002141"
                     /pseudo
     CDS             16827..17092
                     /locus_tag="LZB08_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6716 a         1806 c         2977 g         5593 t
ORIGIN      
        1 atatattaat aattggtagt aaagtatatc aaataagata ttgctattat gtagtaaatt
       61 atataaaaca atgtatcaga ataaatagta tattaataat ttgtagtata tcatgacgaa
      121 taatacagtc ataacaaata gtgagtcata acaaataatg tatttataat tgataatgta
      181 tttaaaaatc aaaatataaa aacttataag gaaagagaga ttaatttata tgggtaaatc
      241 aaaagatgtt gaaatagaga agtctatttt aggcacaatt cttttagaca ataaattaag
      301 ctataagcta gatgaattaa atgaaaacat gtttatgaat gatatatgtc ttgagatatt
      361 taaaattatg aaagaactta aaaaagaaaa catagtgatt gatgtggcca ctgtgaaatc
      421 aaagatagat agaaaatctc tagcgataaa aacaagtgat gtgactaatc taattacttg
      481 gggtcagaat tttggtcttg atggtcatat taaaatttta aaagaaaatt tagctagaag
      541 aagcataaat caaaactgcc aaaacctttt acacagccta aaccttggag aaaatatcga
      601 tacttgtatt tacaaatttg aaagtaatat aaaggagatt ttggacaagg atacttatga
      661 aaatgatgat gtaaatagta tagcagggaa ggtgcttgat tttttagaaa acaaaaaaga
      721 tattggattt aaatttggta taaaattact tgataccact ataggaggtc tttttaaagg
      781 agagctaacc acaatagcag ctaaatcggg ggttgggaaa actgctttgg cacttcaaat
      841 aatgttaaat tcatttaagc aaggtaaaaa aactttattt ataagtagag aaatgaccag
      901 tgagcaagta tttatgagaa atatatgcag agtaactggt gtaagtacaa ggaatatgaa
      961 atctaaagaa atagatgaaa atgattggaa acttattgta aatgctatag gtgatttaag
     1021 cgaaaataac ttaatttata ttaatgataa gatagataca attagtgcaa ttagaaagag
     1081 gataaggcag gtaaagccag atttattaat agtggattat gtgcaactcc ttacatctca
     1141 gaaaagcatg gataagagag aaagggaggt agctactttt tcgagagaac ttaaaaatat
     1201 gactttagat tttaatatac ctgtaatcca attaagtcag ttaaatgatg aaatgaaaga
     1261 ttcaagacct tatggagata gacctatgag ggatagcaaa gccatctacc acgatagcaa
     1321 caatgtggtt tatatacatc aattaaaggg gagtgattat gaagaagcag tgagagatat
     1381 tggagaaagt gaggaggcag ttagagcatc agaatacagg ggaattaaga tggttgattt
     1441 aatagttgcg aagtgtagag atggacagac taggcataag catttttgtt actttgggga
     1501 taagttacat tttcaagaac taaattatta ggtcaataaa gtagtaataa caagcttatt
     1561 aaaagtcggg aaatagcaaa tagattataa aatatgaggt gaaaatatgt tggaattaag
     1621 aaaaattata gaattacttg aagaacacaa gatagaagta agtattccaa aggagttaca
     1681 taagtatagt ttattggaat accttgaatt gtgtcaacaa agtgggggaa catattttta
     1741 tattaaaaca ctggaagggg ctttaaaaga ggctagaaat gagcttatga ttaaattgtt
     1801 taatgggaaa aattatatgg aattagcgag gatgtttgaa atgagtgtta ttaatgtcag
     1861 gcatattgtt agaaaaaaat agatattttt ggagaataca agtgtgatat acttctgaaa
     1921 tagaagttat tatagttgaa ataaaattgt ggttaataaa ttaaagttgt gtataagttt
     1981 aagattatag aggagtataa gtgtgattaa cagcatgatt ttgtgaataa aattgaggat
     2041 atatatgagt taaatgtgtg attaataagg taaggttgtg tataagatta tgagtattta
     2101 aaaggagggg ctataattaa tagtgtagag ttgtgtgttt gcttaagatt atataataat
     2161 ttaatagatt aaactatact aataaggtaa aatcgtgtgt ttagttaaag cttattataa
     2221 aatcttgata tgtgaataaa gttaagatta taatagattt aacctgtggc taatagaata
     2281 aaattgtgaa taaagttaag attataatag gtttaatctg tgactaatag agtaagagtg
     2341 taaatagaga taaacttcaa tatgagctta acttgtggtt aatagagtaa ggtgtaaata
     2401 gagataaact tcaatatgag cttaacttgt ggttaataga gtaaggtgta actaaagata
     2461 aacttcaata tgagcttaac ctatgattaa tagagtaaga atgtaaatag agataaactt
     2521 caatgtgggt ttaacttgtg gttaatagag tagggtgtaa ctaaagataa acttcaatat
     2581 gagtttaact tgtgattaat agagtaaggt gcaactaaag ataaacttca atatgagctt
     2641 aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaacct
     2701 atgattaata gagtaagaat ataaatagag ataaacttca atatgggctt aacttgtggt
     2761 taatagagta aggtgtaact aaagataaac ttcaatatga gcttaaccta tgattaatag
     2821 agtaagaatg taaatagaga taaacttcaa tatgggctta acttgtggtt aatagagtaa
     2881 gagtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     2941 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3001 taaaattata gagctgtata agaaaatatt atagatatat taaatattag ataataaata
     3061 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3121 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3181 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3241 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3301 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3361 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3421 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3481 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3541 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3601 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3661 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3721 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgctaa tatgcttttt
     3781 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3841 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3901 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     3961 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4021 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4081 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4141 catagattta gtttgtgtaa ggcaaactga tttatgcttc ttttttatgt cttagattat
     4201 tggctaagta gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4261 gaataataat taacattata tgaatggata ttcattacat ataagccagt taatagcaag
     4321 atttatttaa tatgaaatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4381 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4441 atggagaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaaataaatg
     4501 taaatatcaa ataagatact atataaaaat agatataagt atctttttta ttattgaaat
     4561 tatgaaaata tatttgactt tacacaaaaa agagcgcctc atgaactaaa aagttcattt
     4621 tgagacacac ttttacagtg tcaaagaacg aagtttatcc ttatccaata cttttatacc
     4681 accacgagat aaagacacaa taccttctct agcaaaatat ttcaacattc tcgatacaac
     4741 ttctctggca ctacccatat acttagcaat ttgttcatga gtcatattta tagtgctact
     4801 gccattttta gctatctcat caattaaaaa agatgccaaa cgcttatcaa aactcataaa
     4861 aagaatttgt tgcatagccc acataacatc agaaaaacgt tcggtagcta atttatatga
     4921 aaataactct gcatgaatat tttctgtaga taattttgca aaaacagagg aactaatctg
     4981 tataatatcg caatccgttt cagcatctac atagacatca aaagttattg tttttagaat
     5041 acaggaagct gataaaatac atacatctcc atcatcaagt cggaacagtg taacctctcg
     5101 accttcatct gaaagtatgt atgtacgcac tgttccagac tttataatca aaataccaat
     5161 acagtcacta tcaccacaat gtactggatt cccttttttg taatgcgagg tatttgctga
     5221 agaaattaat aattcttttt gtaagtcagt aagtttatcc caaaaaggca agctatgtgt
     5281 aagtatatca atttcatttt gtgaaatcat acaatatctc ctttcttaaa ttgttataat
     5341 cccatgatac aagtgaatag ataaattgac aagtagtttg ttaagaggat tatttagtca
     5401 gatagtaata gtatgattta tgtagaaaaa atatgataat atatattctt gctattaatg
     5461 agaaagtaat atgttttgat gatagtaggt ttgtataact aatgcatacc gtttattttt
     5521 aaaagattaa gttccttatg taaccttata gacataaaac cagcaattat agcagccatc
     5581 aaatcagcta ttggttgtgc atataagata ccatttaacc catagaatac aggaataatc
     5641 aaaattatag ggataaaaca gacaccttgt ctacacatac ctaaaaatcc accttccttt
     5701 gcttttccta gtgcgaggaa taatgtagaa tacactgtat agaatccaaa tagcataaat
     5761 gaaaacccat ttgccctaag agctttacta ccattttcta ttaatagaat gtcaccactt
     5821 gtaaattgtg aaattataga ttttgggaag agtatcaata taagaccaag cactacacaa
     5881 aatattgtag tccataatat ggaagttttt attgctatat gtaatctatc gtagttcttt
     5941 gaaccaaaat tataaccagc aattggttgg aaacctttta taaaaccaaa tacaacaaga
     6001 ctacctaaag atgttattcg tgttacagca cccattccag ctatcacaga atcaccatac
     6061 tttgtagcct ccatattagt taatgtaatg gcaagactag tcaataattg aaatattaag
     6121 gtaggtatgc caatttttag gatttctgaa aaaatttctt ttgaaaaaca gcattcgtta
     6181 attctaaaac taaatatact tttcttgctt aaaatatatc ctacataaac aagtgttgaa
     6241 gcaatctgtg agatagctgt tgcaatagaa gcacccctta cccccagacc aaaagtataa
     6301 atgaatatcg ggtctaaaat catattaagc aaagcaccta ataacatagc acacatagtt
     6361 gttttagcag ctccttcact tgtgacaata ttagtcatag tgacattgaa tatattaaaa
     6421 atagatgaca cgacataaat gctagcataa gatattgcat aaggcataat actttttgta
     6481 gctcctaaca attttaaaat tggattgaga aaaatcataa tcacaataat aactaatgta
     6541 cccactataa tactactgta aatagatgta cttaccactt ttcctgcttt ttcattatca
     6601 ccatgtccta aaagtctggc aatatatgaa gcagctccat ttccaaacaa taatcctaat
     6661 cccacaacaa gctgacctaa tggaaatgca acggatatag ctcccatttg acttgttcca
     6721 agtccaccca caaagtatgc atcaaccaaa ttgtataatg cagttatcat catacctatc
     6781 atggtaggta atcccataga taacaatgct tttgagattg gggcagaccc taataattca
     6841 attcttttgt tactttcttt catgcttaac actcctcttc tattgacatt aagtctaaat
     6901 tataatacta taaattatag taagtatgga aaatagtact ataatttata gtatttgtca
     6961 atagtaagaa aactaaagga gatgattttt atggctacaa tagatttaat tgtattggga
     7021 atagtaaagc aacaacccaa gagtgcttat gatattcaaa aggcagtgga atacaggaat
     7081 atatcacgtt gggttaaaat aagtacacca tcaatttata aaaaagtaat tcaattagaa
     7141 caaaagggat atattgaaag taaaactgtg aagaatggaa aaatgccaga aaaagcagtt
     7201 tattcattaa ctgaattagg tgagacacat tttttagatt taatgtttga tacatctagc
     7261 aaaccaataa atattctttt agattttaat gctgttatag taaatttaaa tagccttaca
     7321 ccagaaaaac agaaattatg tctcaataat attgaagaga atgttcaaga gttaaagaaa
     7381 actattgagg aaaatataag tataaagaca gaagtaccag agacagcaaa agcagtgttg
     7441 gagcaacagt tggtgctagc acaagcattg gaaacctgga taaattcttt aaaagataat
     7501 atgggaaatt agaattagag aagctaactt aataatcatt aaacaaaaac cagcatattt
     7561 aataatgaga tattagaaaa aataaaacaa atgatataaa ctgaaaagta tatttaaaat
     7621 aaagctcact taattgaggg agaatgttaa actttttaag agcaagagca agagcaagag
     7681 caagagcaag agcaagagca agagcaagag caagagcaag agcaagagca agagcaagag
     7741 caagagcaag agcaagagca agagcaagag caatcagtaa agtataaaat tctattacta
     7801 taataaaaga tagaactaga agtctttgtg aaagtgggaa aagtatttat ataaataaat
     7861 tcatatagtt tatagtttaa cagtaggaaa tatcgtatga gtgagaatat cgtattttac
     7921 ataaagattt agcaatatga cacactgttg tcgtgttctg taggatatga ttaataaatc
     7981 agatatgaaa ggagagtgag taatatgcca gaattactaa aaaatatgta taatcgtgag
     8041 tcactttatg aagttgcagt agctatccaa tcagtttaca actcatttaa ggtagacgag
     8101 ttcataaaat ctacaatgga tgagacatgg aataacttag aattaaaagc cagatgtcga
     8161 aagataagta tgagtttagg aatgtattta ccagaagact ataaggaagc tctttccatt
     8221 cttgaaaaat cagttacagg tttttatttt gcttttttct ttccagattt tgttgaagtt
     8281 tatgggcagg acaacataaa ttgggattta tctataagtg cattagaaag aaatactgag
     8341 tattggtctt cagaatttgc tgtaagagca tttatcatca aggatgaaga gcgtatgatg
     8401 gcacagatgc gtaaatggtc taagcataag agcgaacatg tacgaagact tgcaagtgaa
     8461 ggttgtcgtc cccagttgcc ttggggacaa gcaatatcca aatttaaaaa agaccctaca
     8521 cccgtactgc ctatattgga gcaacttaaa acagatacat caacgtatgt acaaaagagt
     8581 gttgctaata atctaaatga tatctcaaaa acacatccag atttagtcat aagcattgca
     8641 aaggattggt acggtaaaaa taaatctact aactggattg taaagcatgg ttgtagaact
     8701 ctactaaaaa aaggcaatcg tgatgtatta gccctttttg gatatgatga tactacttct
     8761 ataaatcttc aggactttac tttagaaaca acgtctattt ctattggaga ggatttaact
     8821 ttttcattta atattttagc aaaaaaagcg acaaaaacaa ggctcgagta cggaattgat
     8881 tatattaaat ctaatggaaa aaggaatagg aagatattta aaatatcgga ggtttcatta
     8941 aaagaaaatg agaaaaagtc atatatgaaa aaacattcat ttgcagatgt aagtgtaagg
     9001 aaacattatc caggtataca ctcgattgct attattatta atggtataga aaaagataaa
     9061 cttgattttg aattaggtat ataattttac taacagaata atctatatca gatactttgc
     9121 taaacttata taatatattt tactaaattt atattaggta gtttactact tgtttactga
     9181 atttaaatac aaagaaatac aggtgctaat ccctgcccat taaaggaata tgagcataac
     9241 acaaaagtag aacttatcaa ttctgttacg ggtatagtag taaatccata gaatcacaat
     9301 tagaagatga agtgtaaaaa ataagttagt gttgatttta gttgctagtg atattaaata
     9361 gttatacaat agccatataa attagaattt gtagaggaga aatgtaaatg gatgtatata
     9421 gtttttggaa tgacactttg gcacaagatg ctgttaagat aagaaaatat tttcatgaag
     9481 atgcttatat taattggcat gatacaaatg agcattttac tttagaagaa tttattattg
     9541 caaattgtga gtatcctgat aaatggaatg gtaaagtaga aaaggtgcta gaatttggaa
     9601 atttagtaat tacagtgaca catgtttatg caataaataa atcactatca tttcatgtcg
     9661 tttcatttat cagccttaaa gatgataaga ttatttctat tgatgaatat tggggagagg
     9721 atgatgatgt gccacagtgg agattagata aacatattgg aaagccaata aagtagcgtt
     9781 tgttctgttt tataactgaa attttgataa catttgatac tttgatttgt cattggtcaa
     9841 catagtcgtt agtgtaataa tttaaaaaag tagaatttgg agagtgagca ctatggaaga
     9901 aaaatacatc ttatctgacc gagggaaagt atattattgg ataaaagaaa gtaatagtgt
     9961 gtttgcaaag aacttaattt ttttgccagg actaacagca aatcatcatc tttttgattt
    10021 ccagatacag tacttttcaa aaaatcataa tgtattagta tgggacgctc ctgctcatgg
    10081 aaaatcaaga ccttacttag atttctcata ttctaacttg gtagaagaat tgaaagtaat
    10141 tttagataga caaaatatga aaaaggttgt acttataggg caatcggcag ggggatttgt
    10201 ggcacagtcc tttattgtaa aatatccaaa tatggtagaa ggattaatga tgattggaac
    10261 ttgtccatat ggaactgact attatagtaa gtcagatatt ttctggttaa aacagactga
    10321 atggatgtta aagttattcc cagataaatt gttgcgaaac ataatatcta agatgtgtgg
    10381 agctacgtct gaaggcagaa ggagtatgat gaaaatgtta gaaggctacg aaaaaaaaga
    10441 actgtgtcat ctcatgtatc tgggatttgc tgggttcata ccagaaattc aagacttaaa
    10501 tatacattgt ccagtttgtt tggtggttgg agataaagac aagacgggta aagtgagaga
    10561 atataatgag cagtggcata ataagactgg gtatccattg cacattataa aaaatgcttc
    10621 acataatgta aatgtcgata aacctaatga actaaatcag attatggata tatttattaa
    10681 gcaattataa tatataaaaa ttacatactc attaggtaaa ttatgtagta tatgtgttta
    10741 tagatggtta tagaaataaa tatgtaataa actacttaac acttaaaaag tggatttgtc
    10801 cagagcataa tacaaagcat gatatataaa tttttaagcg aaatacatca aaagaagtat
    10861 tagaattagt ggagataaaa cagctattag agattataaa taaatagttt taaaggtact
    10921 acgaatatag aggaaggtaa atcatacgtt attcaaggat tataaatgat tagtttaaaa
    10981 gtactatcgg ataggagtct ccatatttaa accttagtaa aggtagttaa agtagtagtt
    11041 taaaaactaa aaaattatat aaaggaatta ggtataagaa aatgattgag agtataccag
    11101 caaaacaaat tttacaaaaa gtgaaatttg acaatacaag atggtttgga attgactata
    11161 atatgaatct ctatcgtggt tgttctcatg gatgtatata ttgtgatagc cgtagcacaa
    11221 tatacaacat agagaatttt gacaaagtta gatataaaga aaatgtgata gaaattttaa
    11281 gtaaagaatt gcgttcaaaa agaaaaaaag gggtagtagg tattggagct atgtctgaca
    11341 catataatcc ttttgaaaag caactttgta ttacaaagca agcactagat ttgatttcgg
    11401 aaaatcattt tggagtgtct attgatacta aaagtagttt ggtggttaga gatataccga
    11461 ttttgcagaa aattaaaaaa aataattctg ctattgtaaa gttgactata accactgcaa
    11521 atgatgaatt atcaaagaaa atagaacctt atgtaaatcc aagttctgta cgatttgagg
    11581 cagtgaaaga acttaatgat tctggaattt tttgtggcat attattaact cctatgcttc
    11641 catttttgac tgataataaa gatgaaatac gagcaattgt agaaaaagca cacaaggcta
    11701 atgcaaaatt tatttattgt atgtatggtg taacaatgcg tagtggtcag agagagtttt
    11761 tttatgagca tttaagagat atatcaccaa aattggtact taaatatcag aaaacatatg
    11821 gtttaaacta tgtatgtact gtacaaaata aagattgttg tgagaagttg ttaagagaag
    11881 aatgtgcacg ttatggtatt ttgacggata tgaaagagat tataaaagcg tacaagaggt
    11941 cggagtcata tactcaaata aaattttttt aagataggct ttggatgaga tataatagtg
    12001 gtgattgatg tgttgatatt atagttgatt gttagagagg atattataga tgaatattag
    12061 tattaatatt ttaggtaatt gctagagctg atattataga tgaatattaa tattgatatt
    12121 ctaggtaatt gctagagctg atattataga tgaatattaa tattgatatt ctaggtaatt
    12181 gctagagtta gtattataga ttaatattag tattgatatt ctaggtaatt gctagagctg
    12241 atattataga tgaatattaa tattgatatt ctaggtaatt gctagagcta atattataga
    12301 ttaatattag tattgatatt ctaggtaatt gctagagctg atattataat ttattattag
    12361 tattgatatt ctaggtaact gctagagctg atattataat ttattattag tattgatatt
    12421 ctaggtaact gctagagctg atattataat ttattattag tattaatatt ctagggaatt
    12481 atcactatat tttaaaaaag taaaaatata tatcaaaatg aactaaataa gtactgtaag
    12541 aagtttgcta aaataaggtt tgaataaagt gagggtggag aacttatgat agaaataaaa
    12601 actatagaaa taaataaaaa aagtcaaatt gaccttttga tgttagccga tccatgtgaa
    12661 gaaatgattg ataaatatct caataaagga actatgtacg cattgtatga taataaagaa
    12721 cttacttgta tagcagttgt aaatgaaatt tccaaagaaa tatgtgaatt aaaaaacatt
    12781 gctacatatg aacattttca aaatatgggt tatgcctcaa agatgattta tcatctttta
    12841 gatgtttata gtaaaaaata ttcatcaatg gttgttggta catctgaaag cggagttcct
    12901 ttttatgaga agtttggatt tgtttattca cataaaatta aaaacttttt tgtagataat
    12961 tatccagaac caatctttga aggagaatta caatgtgttg acatgttgta tctatcatat
    13021 aacttttaga taggataata tttttaggtt aatataatta attaattcat ataattatcg
    13081 gaattataat ttataaatta tgtataataa gagtataatt tatttagaag ctgttacatt
    13141 gtattatatt caaataatat atataataac ttgtttattt tgaattttgg aggattaatt
    13201 atggattcaa ataataatac tataaaatca actgttaaaa agggtatttc ttttggttct
    13261 tgtttagcaa tgattatttc ttatactgca tggaaatcta ttccatgggc tatttttcat
    13321 ggcttaatga gttggatata tgtactttat tattgggtta agtatgcata gattaaatta
    13381 ataaataagt atgtaatatt gtgaagttta agaaacaaaa cgtaagtgag tgtctcgaag
    13441 tgaactttta gtttataagg cactcttttt tgtattaaat aaaatatatt ttcattactt
    13501 aaacaatgaa aaagatgagt atctctattt taatacatac ccttatattc attcatttat
    13561 caacaagagt tttaaaaaga aagctatcta aaatctattg taatctaagt gcttctcttt
    13621 aaagtcttaa ctttagtaat gatatataag tttataaggt tcttatttca ataatgcaaa
    13681 ttttacttag aagttcagac tttggtatga agaaatgggt aataataaaa aagaggtgtt
    13741 gatatgaaag aaaaattaga agaaaaatct aagattatta aaacatcaaa agagtataaa
    13801 cgaactataa ttgaagaaaa ggacagagaa tggaccaaaa aattaaatga catacttagt
    13861 agaggtgatt tttctaatga aaaattagat aaaatcaagg atttaattcc aaaagaaata
    13921 ttgactagtg aaaatgttgg agaagttgta actgaagatg ggtatgctaa gcctgagtat
    13981 gatgaggtat ttaaatcatt gtttacttta aataatgatt acgatttact tgcaaatttt
    14041 ataaatgata tcctcaaaga tgctccatat gctaatagga atatcaaaca gttcactcaa
    14101 ataaaaagaa taattaaggt tgaaaccgac ccaacaatta attacattgg tgagaaaaga
    14161 cctagacttg atattttggc tgaagatgaa gaacgtaacc atataaatat tgagatgcaa
    14221 agagctcttg aagaggatta tttggaaaga gctgaatatt atcttagtcg tgtacatggt
    14281 agaaagttag aagaaggtaa agaatataaa gaaattggaa aaactgttgg aatacatata
    14341 ctaaatcatg taaaatataa tcacatagaa gattatgtaa attgcctgag attaacaatg
    14401 gacgggcatc cagatatttt ttctagtaaa actgcattat attttataga acttccaaaa
    14461 attcgtaaat ccagttgtat agcaaacaga gttctaatat ggggtaaact tattgataat
    14521 ccttctcata tagatattag aatactatct aaaacggatt atgttattaa aagagcactt
    14581 gatagattaa aagaattagg tagtaatgag gactatttat taaatttaaa gagaggtgcg
    14641 tatattatga ataaaagtag aaattttaaa gaagaaattt ttcaagaagg tattgaaaaa
    14701 ggtatcgaaa aaggtatcga aaaaggtatt gcaaaaggga aaagagaaga gaaatttaat
    14761 atcatcataa aattaaaaaa taaaggatac aatttgagtg aaatatgcga tattatagat
    14821 gatttgaata aatcagaggt tgaaaaagtc tataatcaaa actaatacta tgattgagat
    14881 ggcactagat aaatacaaag cttcctcttt taaatttaaa ttaatataaa actttgttat
    14941 ttaaatatgg tagatataaa gagatatata aataataggt acaaagaggt gtataaataa
    15001 aggagataat tcaaaataaa agtttgaacc atctccttct tatagaaaaa caaaggggtt
    15061 gatttatata tgaaatttta tactatacag actttagaat tttggaataa caataaaaac
    15121 aataaatatc tatcaaatga ttattctttt atattagaag actttgatat tccatacaaa
    15181 tggatgtata gtcaaatgac aaatagaata tatgaatttg atgatagtat gatatgggtt
    15241 tggacgaaga gacctgattt aagatgtagt ggttatttag aaaaaggaaa aaaaggtgtt
    15301 ttacttgaaa tagaaataag tgaagacaat gtgttattat cagaatttga tttatggcat
    15361 ttacctttaa tggatattgc tgtagagctt tatgaagatg aacaaattga aaagaaggta
    15421 agttgggaaa gaatatttga cttagaactg tgttcaaaat taaatgaagt taatatagaa
    15481 gatttgacat tgcaaggagt gacatctaaa gtgcctctat ctaatgtaaa attaataaaa
    15541 gaatttgttg caaaataata tttttattga aatttattca aaaaagttat atatttataa
    15601 caagaggtgt tataaataca taacttttta agggggattt tatatgtcta aacaacaaac
    15661 tggtattaaa tcaacactta tttcagaaat attgggaatt tcttattgta ttggagctac
    15721 attagtaaat aagtattttt ttaaaggaag tagtattgaa tatcttccta taattatttg
    15781 ggtattagct atgggagctc agtgggtcta taataaaaag tatgatgatt taattgatga
    15841 ggcatcaaaa tgtatactat caagagttaa tgatattgca ataaaagtat tgtttttttc
    15901 tgtagctata gtatctatat ttcttgtaac tttggacata aatgtgtcaa atttaaatat
    15961 aggaatgtct ttattagtca ttttgtttat tcaatccttg ttgaagttaa ttctatttat
    16021 atattttgac agaaagggga tatacaatta ggtgtcagta ttactaaaga caaaaattaa
    16081 atcctataga gaaaagaacc ttatgacaca aggtgaactt gcaaagtcag taggagtaag
    16141 acgtgaaact attgtacatt tagaaaatgg tcgctacaat ccttctttaa aactggcaat
    16201 ggatattgct aaggtatttg atacaactgt agaagagttg tttgaattta ttgaagaaaa
    16261 taatatttaa atttcatgta ttacttatgc attagagaat gaattaatta taggtaaaaa
    16321 accaagctta tagattagag aataaatgac ttatagatga aaaaccaagc ttatagatta
    16381 gagaatgaat taattatagg taaaaaccaa gcttatagat taaaaaatga atgatttata
    16441 gatgaaaaac caaactcata gattaaaaaa taaatgactt atagatgaaa aatcaagctc
    16501 atagattaaa gaataaataa cttattagat gaaaaaccaa gcttatagat aagaagtcca
    16561 tgcttataag ccagaaaatg aatactttaa tagattaaaa aatacatatt tgtgaattat
    16621 agaatgaatg actccataaa ctagaaaatt tataataata tctatatgaa aattagaatt
    16681 attttatcac aaaaaaatta agttaaataa atatgaaatt acttccaaat atacttccaa
    16741 aaacatgtaa tcgcatatat aatataatta taaaaataat aaatattttt atagtagtag
    16801 ttaattatta ataaggaggg gtcaaaatgc ctagcatgat tatggaagtt gtaggagtac
    16861 tatttatagc tattggatta atcttgacat tcattgaaat aaaaggaaat ttcccttctt
    16921 tttatggttt tggtcaaaat taaaatgctg taggtattat agcagcagga attgctttaa
    16981 ttttctttag aaatggcata gatagcatag attctatagc tacttatggt gtatttttag
    17041 caatagaact tgttgtaata gttgcatatt tcttaataag aaaaaaattt ac
//
LOCUS       DAFKMX010000085        17049 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFKMX010000085 DAFKMX010000000
VERSION     DAFKMX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.085"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            242..3145
                     /locus_tag="LZB08_002142"
     CDS             242..3145
                     /locus_tag="LZB08_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315640.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3178..3624
                     /locus_tag="LZB08_002143"
     CDS             3178..3624
                     /locus_tag="LZB08_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315641.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3747..4049
                     /locus_tag="LZB08_002144"
     CDS             3747..4049
                     /locus_tag="LZB08_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315642.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4230..5636
                     /locus_tag="LZB08_002145"
     CDS             4230..5636
                     /locus_tag="LZB08_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBM7315643.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5980..7038
                     /locus_tag="LZB08_002146"
     CDS             5980..7038
                     /locus_tag="LZB08_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBM7315644.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7137..7823
                     /gene="araD"
                     /locus_tag="LZB08_002147"
     CDS             7137..7823
                     /gene="araD"
                     /locus_tag="LZB08_002147"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBM7315645.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7841..8488
                     /locus_tag="LZB08_002148"
     CDS             7841..8488
                     /locus_tag="LZB08_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBM7315646.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8661..10028)
                     /locus_tag="LZB08_002149"
     CDS             complement(8661..10028)
                     /locus_tag="LZB08_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBM7315647.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10218..10306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10734..12854
                     /locus_tag="LZB08_002150"
     CDS             10734..12854
                     /locus_tag="LZB08_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBM7315648.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12958..14211
                     /locus_tag="LZB08_002151"
     CDS             12958..14211
                     /locus_tag="LZB08_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBM7315649.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14365..15426)
                     /gene="ytvI"
                     /locus_tag="LZB08_002152"
     CDS             complement(14365..15426)
                     /gene="ytvI"
                     /locus_tag="LZB08_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBM7315650.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15700..16440
                     /locus_tag="LZB08_002153"
     CDS             15700..16440
                     /locus_tag="LZB08_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBM7315651.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16452..>17049
                     /locus_tag="LZB08_002154"
     CDS             16452..>17049
                     /locus_tag="LZB08_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBM7315652.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGL"
BASE COUNT         6855 a         1866 c         2930 g         5398 t
ORIGIN      
        1 ttttcaatca taaatttata aagtattttg cattaaaatt taaacaaaga gttaaattat
       61 ttaagattat gaataactaa tacatttagt aaattactaa atatacaaaa tgtaaatact
      121 tatcaaaatg tataaatact tatgacatct agttacttac agggtttaag tagaaaaaaa
      181 tatcaaagta agtatggcat gatagttgct tatatattta tcaagaaagg agttattaag
      241 catggagttg agagataaac ttgtaaacat cataaataca gaaaataaaa aagatccatt
      301 aacagatgtt caaatagcaa agtttttaag tacaactaga gagaacataa ctaatttgag
      361 aaaagaactt aatataagta attcaaggca aagaagatat ccttatttaa aatcagcaat
      421 ttcggcaata ttacaaaaga ataagaatat ttctatatca gaaattacaa gggaattgat
      481 gacagaaggc tttaatatat ctagaagagt tgtggaagaa cttttaccta aagaaagttt
      541 agaaatggat gtagtagagg aatctgaaga agaatcgaat gacccatttg aaactctgat
      601 aggtaataaa gggagtctta gaaatgctgt agagcaggct aaatctgcta tattgtatcc
      661 accaaaaggg ttaccaacgt tgattattgg ggaaagtgga gttggtaaga gtttattttc
      721 tagacatatg tatgagtttg caaagcaaaa aaagatagta aaagagagct ccaattttgt
      781 tgtatttaac tgtgctgatt atagtgacaa tccacaatta ttattatctt tgttatttgg
      841 atataaaaaa ggtgcattta caggtgctga atatgataca ccaggtcttg tagaagaagc
      901 tgatgaagga gttttatttt tggatgaaat acatagattg cctccaaaag gtcaagaaat
      961 actattttct attttagata ggggaaagtt taggagatta ggtgaaacta atgctgaaag
     1021 aaaagtaagt attatgttga ttggggcaac tactgaaaat gtagagacta atttattatt
     1081 aacattcaga agaaggatac caatgctaat aaccttgcca ccattacatg atagattact
     1141 aaaagagaaa atagacctta tatacaatat ctttcaacaa gagtgtaata gaataaatgc
     1201 aaaaatattt gtagataaaa atgtgattga aatacttgca ctaaaaaagt ttagtggaaa
     1261 tataggacaa ttacaaaata tgattcaggt tctttgtgca agagctttta tgaagtttat
     1321 aaattccaaa gatgaagata gtgaatcgat agtagtagta gatataaatg aagttttaaa
     1381 gcttaaagat tcttttaaag atgctgcctt tcaagagatt gaatatacag agataagaaa
     1441 atatttgaaa aatgctatat ttataccatt taatttagaa gaatcatcag taaaaggaag
     1501 tcttttaagt gatgaatata tattaccaga ggacatatac aaaacaattg aaaagaaata
     1561 ttatgaccta aaatccctag acattgaaga tatagatata gagaatattt tatggacttt
     1621 tattttaaat agatttgctg acttaaagtt taatctcgat agtaatgaag aaattttttc
     1681 aatgagtgat ttaaatagtt ttgtaagtga tagtttagtt aaactggtaa aagaacttat
     1741 gaatgatata gttgaaaaaa atatgaattt agaagtaaat aaaaatatgt tcaaatatct
     1801 agcaatacac ttagaagaag ctataaaaag aattaagcta aatcaaaaga taataaatgt
     1861 aaatttagaa aaaataaaga ttgacttttc aaaagaatat gaaattagta aaagttttgc
     1921 aataaaaatt gaggaatcaa ttggtattaa gatttcagat gatgaaattg gatttataac
     1981 gctctacata aagtctgctc taaaaaatga agtgaaaaaa gataaagtag ggttaataat
     2041 aatttctcat ggacgtatag ctacagagac tgtaaatgta gttaaagaac ttttaggtgt
     2101 aaagtttcct gtggctatag atatgccttt agatgaaaaa ccaataaata tatacaataa
     2161 ggcagtagaa ctgtcaaaaa taatagacca agggaaaggc atattgtttt tggtggatat
     2221 tggttcactt acaaacatag gtcaaatagt aaataagaga accaatataa gtactaaaac
     2281 tattgataga gtagacctcc taatggcact agaagcaact agaaaagttt ctatgggaga
     2341 ggaagaactt gatgaaatat ttttttcttt gctaaaagat aggatgggat ataactatga
     2401 attagaaaaa cacatagata aatcaaatgc aattataaca ttatgtctta ctggagaagg
     2461 aactgctaag tatataagta aaacgttgga agaaaagtat gataacacta aatgttatca
     2521 aatgagtgca ttggatgaaa atttattttg taaaattgaa aatctaaaag agacaaacaa
     2581 tatattagct attgttggga caataaaccc taagatacca ggtataaatt ttatacccta
     2641 taataaagaa cttttcaaaa atttggatat ctatctatca aaatcaaaac aacaagatga
     2701 cagaccagtt aaatcgtatg aaagaatgct tgatgaggat ttagtgattt ttgaaccaga
     2761 tatatatttt aaaaaggatt tattagaata tgtatgttca attcttataa ataaagaata
     2821 tgtagaaaaa gattacttag attctgtttt acatagagag gaaatgcttc caacatattc
     2881 taaaggagct ataggtgtgc ctcatggaga ttcttctact gtaaatagta caaggtttgt
     2941 ttttgttaaa ctaaagaatc ctattgattg gggtgtaggt aatgtaaatt tcatatttat
     3001 gccagttttt caggctaatg ataaagagat tgttaaaaat gttcttgaga tactaaaaga
     3061 tgcagaattt atgaacaatg ccaataagtg ttttgataaa gatgctttta gaaggattat
     3121 atttgataaa tttaaacatc tgtagccata gcaaaaaaat tgagaggagt tagagtaatg
     3181 aatgtagatt taatatcatt gggtttagaa tctaatgatt atgaaagtgt tattgaagaa
     3241 ttaggaagta taatgtgtaa aaaagaatat gtaaaagaaa catacataaa tgctgtcttg
     3301 gaaagagaaa ggacattacc aacaggtcta gatattggag acatgtgtgt tgctattcca
     3361 catacagatt caaagcatgt aaatgagtct aatgtagctg tagggatatt gaaaaatcct
     3421 gtaaagttta attcaatgat agacccaaaa gatagacttg atgtagaact tgtattttta
     3481 ttagcagtaa agaacccaga ctcacaagta aaactgttaa aagatttaat gtcagttttt
     3541 caaaatataa agttattaaa aaatataaaa aacgcatcaa caaaagaaga agtagctaaa
     3601 ttattggatt ttatagaaat ttaatgtagg agaattttat aaataaaaaa ttaaaattta
     3661 aacaatcaaa gtaagtatta tttttttaat aggtttaatc ttatataaaa taaaaattta
     3721 gagtgaaaaa ttaggaggaa attattatga aacaatttaa tgtattatca gtttgtggtt
     3781 cagggacagt tacatcatct atggtagcag gtaaattaaa agaagtacta ggtaataagg
     3841 ggatttctat tactacaaca gaagctagac caacagaagc actaaattta gcacaatcag
     3901 gaagatttga ctttataaca tttacgagtc cacttcaaac aggcgattat ggaataccaa
     3961 ctataaatgc atttgcttgc ctaactggta taggggaaga ggcattcttt gaagaagttt
     4021 tgactgttat tgaagggata gataagtaag ttttggagac gtgaatttat agtatttaaa
     4081 tcaatgattt agatactata ataagctaat attaactaaa tatttgtgga aaaaacattt
     4141 tatatgttgt cataaattaa aaaattataa aaatagtcac ttaagtatta aattaagctt
     4201 ataaatattt ataagtagga gggaaaaaga tgttagaatt tatgaaatct ataatcgata
     4261 catttggttc tgcaattata gttccaatta taatattcat aatcgcaaag atatttaaag
     4321 taactactaa gaaatcgttt ctttctgctg tgtatgcagg agttgcttta caaggtttta
     4381 cactgatttt aaattcattt acaccagtaa taacaccagt tatcaatagg atggttgaga
     4441 gtacaggagt aaacctacca gtttttgatg ttggttggca agcaacatca ttggttgcat
     4501 tttcaacaag tgcaggtatg atatatttag gtcttggtat attattacaa acaattttat
     4561 ttttgataaa atggacagat gtgtttcaac catcagattt atggaataac tattcttata
     4621 tggtatgggg agcaatggtt ataggagtaa caggaaattt tcctttaggt atagcatgta
     4681 tggttttatt aaacctatat agtttattaa tatcagagtt agttgcaaag agatggtcta
     4741 gttactatag atatcctaat tgtacaataa tagctatgca taatgttgaa gcttctgtgt
     4801 ttgcagtatt tgcagaccct atatacaata aattgggatt aaataaaata aaattaaatc
     4861 caaaagaatt agagaagaaa ttagggtttt taggtgaacc aattacttta ggtctttttt
     4921 taggtatgtt tataggtata ttaggtaata tgacgagaat aaatactatg gaagcttggg
     4981 gagaaataat gaaagtaggt atatctacaa gtgcagtgat ggcaattttc cctaaagtag
     5041 caagtatgtt tgctcaagca tttgcaccta taacagaagc agctagaaag attatgcaaa
     5101 aagctggaaa ccgtgagtgg tacatagcag taaatgatgc tgttggatat ggtgaacctg
     5161 caactttaat atcaggtctt atattaattc caataatgct tgtaatagct atggtacttc
     5221 ctggtaataa ggtattacca gttgtggatt tattggcaat tccatatatg gttcaaggtt
     5281 tagttgccat acataatggt aatataccaa aagtattagt ttcaggtata atttggtttg
     5341 gattaggatt atatgtttgt acatctacag caccattatt tacagatatg gctacaaata
     5401 taggtgtagc tatacctgca ggagcaatgt taataacaag ctttaatata ctaggaaaac
     5461 ctttgatggg attagttttc tttgcatttc taagtgcaaa tccaatttat ataggtcttt
     5521 cagttgtaat ttactttgtt ctatgggcat tgtttagaaa gaacaaaaca agtattcttg
     5581 attatttaga aaaacaagca ctcaaaaatg tagaagaaga gcctgtagca gtttaagttt
     5641 ataaaaatga aataaaaata attaaaggag tttataattg attttatata agtatcagat
     5701 aatacctgag aaatatttaa atgaaatata gacttctttt tatttgataa tatttgtaat
     5761 tataatgcat tgtattttta tagttgagtt ttctgtataa cctgctattt aaaacatact
     5821 cttttatatt tatacagtaa tgtggtttaa ttttataaaa tgtaatctct actggtaaaa
     5881 gactttgtat ataaggcatt tgcctgaata aaccatttat cttttgtatg gcatagatat
     5941 tgctatatag aagtatatag tatgaaagga gatatttata tgggagataa aatgagagca
     6001 tcagtattgt ataatgtagg tgatgtcaga tatgaaatgg tagatatacc agaaataaca
     6061 gatacgcaag tgcttgtaaa tgtaaaatat gttgggatat gtggttcaga tttacctagg
     6121 tctatggtaa gtggtttgag tggaaataca aaatatccat tgattttagg gcatgaattt
     6181 tctggtgaag ttgttaaaat tggagaaaaa gtaaagcata ttaatgtagg agatagagta
     6241 gctgttgcac cattagtacc ttgtggaaaa tgtgattatt gtaatgaagg taattttggc
     6301 ttgtgtgatg attacaatat aattggaaca agagtaaatg gagcgtttgc agaatatgta
     6361 agagttccag aagaacatat tttaaaacta ccagatacat tggattatga aactgctgcc
     6421 ggaattgaac cagcaactat agcttatcat gggatttcaa agtctaatat tagagtagga
     6481 gatagtgtag ttgtgttagg atgtggtcca ataggtcagt ttgttataca atgggcaaag
     6541 gtgtttggtg catctaagat tatagcagtt gatatttttg atgaaaaatt agaactttct
     6601 aaacttctag gagcaaacta tatacttaac tcaaaagaag taaatgtaat caaagaaata
     6661 aaaaaaataa caaatggtgg tgctgatgtt gtaatagaaa ctgctggtag ccgatttaca
     6721 caagaacaat ctctatttgt agcaaaaaag agaggaaata tagtgtttgt aggtatatca
     6781 cacacagaat taccacttag tgcagacgct actgagtgta tattacgtgg agaacttacc
     6841 ttgaaaggtt cttggaattc atatacaagc ccttatcctg gaagagcatg gactgcaaca
     6901 ttggatttta tggaaaaagg tgacattatt tttaaaccca tgatttcaga taaaataggt
     6961 cttaatgaag tgggtgactt tttaagtaaa atgagcaaga gagaaattaa tttcaataaa
     7021 atcctagtag aaatataaaa atttaataaa aaattctaaa agcaattaaa ttttataact
     7081 atgacattaa ataaaataaa aataattttc ttaaataaaa tataggagga agaaaagtgt
     7141 tagaaaattt aaaagaagaa gtattaaaag caaacttaga attacctaag aaaaagctaa
     7201 ttacttatac ttggggaaat gtaagtggga tagatagaga aaaaggatta gtggtgataa
     7261 agccaagtgg tgtagagtat agtaatatga ctgtagaaga tatggttgta gttgatttag
     7321 agggaaatat agttgatggc aaattaagac cttcttcaga taccccgact catctagttt
     7381 tatataaaga gtttgaagaa ttggggggaa tagtacatac acattcaagt tgggcaacaa
     7441 tttgggctca aataggaaaa tctataccat cttgtggtac tactcatgca gattattttt
     7501 atggaagtat accatgtacc agaaaaatga ctagagaaga aatttgcaat gaatacgaaa
     7561 aggaaactgg aaatgtaatc attgaagaat ttatgggaag aaatccaatt cattgtccag
     7621 gaataattgt aaatgaccac ggacctttta catggggaaa agatgcaaat gaggctgtac
     7681 acaatgcagt agttttagaa gaagtcgcaa agatggctta ttatacagag ttaatgagtc
     7741 ctgataatat tatggataag gttcttatga ataagcattt ttcaagaaaa catggaaaaa
     7801 atgcatacta tggtcaaaaa tgattatctg gaggtgaatt atgctagact ctttaaaagg
     7861 taaagttgtg gaaatagcaa ttaaagcaga gcattatggt ttatgtaaag aaaagactgg
     7921 taattttagt attagagata aggatactgg atatatactt ataaccccat caggtgtagg
     7981 aagagaagaa ttaaaagttg aacatatttg tgtattggat ttggatggaa atgtaataga
     8041 agttgagaaa ggtattaagc caagtagtga ggtattgatg catattgaaa tatacaaaac
     8101 aagagaaaat ataaatgcca tatcacatac tcattcttta tatgcaacag cattttcagt
     8161 agcaaagaaa ccaattatac caattgtata tgaatcagtt aattatggcg gttatgtaga
     8221 tattgcttca tacgaaagac caggaacttt aaaattagct aagagtgttt caaaattact
     8281 gatagataca gatgcatgct tattagaaag acatgggttg acaactgtag gtaatgattt
     8341 agacgaagca ttattaaaat caagatatgt ggaagaggta gcagaaatat actataggtc
     8401 tttagtatta aatcaattta aagaacttga agtagtagat gttgatgagt ttaaatcttg
     8461 gaaataccca gaagaaataa agttttaatc taaataattt tcattgaagt ggttaattaa
     8521 aatatatgta ttatttatat caattactat gttttattat gttaattacc gtattttgca
     8581 aatatattta ttacattttt tcaaaaaaat atagaatccg aaagccatat aaaattgact
     8641 tgggattcta tactttttat ttatgcattt ttctttatct gattttctgg agaatttata
     8701 tgttcaaaat attttttagt ttcagatact attataggtg ataatgcaag taaagctata
     8761 aggttaggta ttgccataag cccatttaca atatcagcaa ttatccaaac aacttctaac
     8821 tttaagaaag cacctaataa caccatagcc acaaatacag ttctataccc attcatgcct
     8881 tttacaccaa acagaaattc aaaacatctt tctccataat aactccatcc caaaattgta
     8941 gtaaatgcaa ataatgttaa actaactgta agtaatattg gtccaaatgt aggaattgct
     9001 ccgttaaaag ctgcttgtgt cataacagct ccatttaggt ctgaacacca cactcctgaa
     9061 ataacaagag agaatccagt aagagtacag attataatag tatctataaa agtacctgtc
     9121 atagatataa gaccttgttc cgctggccat tcagttttag cagcagcagc agctattgga
     9181 gcactaccta gaccagactc atttgaaaat acaccacgag cgataccatt tctaattgcc
     9241 agtgacatag ttgctcctaa aaaaccacca gtagcagcag taggtgtaaa tgcactttta
     9301 attattaaca taaaagtttc agggatttta ttccaaaaag atattaaaat gattccacag
     9361 attacaacat atataactgc cataaaaggt actattttag tagctacatt agatatactt
     9421 ttaagtcctc caaatacaac caaagctaca aatactgcta atacaacccc tgtaattcta
     9481 gggtctatcc cgaaactatt atttattgca tctgttattg aatttacttg agtaaaagta
     9541 ccgattccta aaagtgcaac tagtatacca cttatagcga aaaatattgc aagaggtctc
     9601 catttttctc ccataccatt tactatgtaa tacattggcc caccagacac ttggccattt
     9661 ttatctttag ttctgtattt tattgcaaga acaccttctg aatacttagt agccattcca
     9721 aagaatgctg ctatccacat ccaaaaaagt gcaccaggtc caccagcttt gatagctgta
     9781 gcaacaccaa caatattacc agtaccaact gtagcagcta gagcagtaca taaggctgca
     9841 aaacttgaaa catcaccact acctttgttt tcagcagaga atattaactt aagagctctt
     9901 ggcagtttag ttatttgaac aactccaagc ttaacagtaa gtagtatacc agtaccaact
     9961 aatagtacca gtaggggtgg tccccaaata aaagcatcaa ctttgtttaa aatatcaatt
    10021 agattcataa aaacactccc tcattttttt tgctttacat aatcaataga atttttaatg
    10081 aagtaattga caggaaataa gtataattta ttaataattt ctagtgtaat aaaaaaagct
    10141 aaatctaaaa aagattcagc tctggatgta cactaattag caaccaaaat aaaagtatta
    10201 gtaaatactg tttttggtag ataatccctg tccttttgcc tgagagattg agcataaaag
    10261 ccttgcccct tcggcgccca taatgggtct ctccagagtt ccgtaaattt tacagtccta
    10321 cttttaaaag tacctgagag tgttactcct tcggtgtgct atatagcaac tcttctataa
    10381 aatcttaacc ggatattgat ttgtcatagc ttattgtatt aataatatta atataagttt
    10441 ttatgttttg tcaactaaaa aattaaaaaa caaagaaaaa atataaaaat gtaagcctat
    10501 ttacgaatta taaacttaga aagatgagta atatacgtga aaatgaattt tgtaatacac
    10561 atacaaatga agtgtaaaat aagttttaaa taaaaaaata ataaaatctt aaataattaa
    10621 ttagatatga ttaaattatg gtaatattat agttacgata ttaagaaaag ttctatttag
    10681 ttagatttta agcgtaatat gagattatgt atagagaaag gatgtatttt tagatgggaa
    10741 aaatattagt gctagcagaa aaaccttctg ttggaagaga tatagcaaaa gttttagggt
    10801 caaaaaatga aaaaaatggt tatatagagg ggccaaagta tgtaataact tgggcactag
    10861 gtcatttggt tacacttgct gaccccgaaa gttatggaga aagatataaa tcatggagct
    10921 tagaagacct gccaatactt cccaagcacc ttaagactgt tgtaataaaa aagagtggaa
    10981 agcagtttaa tacagttaaa tcgcaaatga atagaaatga tatagatgaa atagttatag
    11041 caacagatgc aggtcgtgaa ggagaacttg tagcaaggtg gataatagaa aaatcacagg
    11101 taaaaaaacc tataaaacgt ctttggattt cttcaagtac agacaaagca ataaaagacg
    11161 gatttgcaaa attaagaagt ggaaaagaat atgaaaattt gtattactca gcaatagcac
    11221 gagcagaagc tgattggatt attggaataa atgcaaccag agctttgaca acaaagtaca
    11281 atgcacagct ttcttgtgga agagtacaaa ctcctactct tgcaatgttg cttaaaagag
    11341 aagaagaaat aagaaatttc aaacctaaag agtattatgg attagaatta attgctacaa
    11401 agggaaattc agatataaaa tttatttgga atgataaaaa caataattct tcaactttta
    11461 gtaaagaaaa gatagaatcc acattaaaaa aagtaaaagg tgtagacata aaaatatctg
    11521 aagtaaacaa gtcatataag aaaaaatatt cacctgcact ctatgattta acagaattac
    11581 aaagagatgc aaataaaatg tttggatttt cagcaaaaga aactttatct ataatgcaaa
    11641 aactgtatga acatcataaa gtacttactt atcctagaac tgattctaga tatttaacat
    11701 cagatattgt tggaacatta aaagatagaa taaaagctgt aaatattagt gattattcaa
    11761 agatttgtaa tagattatta aaaagcaata ttatagctaa taaatcgttt gttgacaatt
    11821 caaaggtaac agaccaccat gcaataatac caacagaaca gaaggtattc atgagtgatt
    11881 ttagtgataa agagcgaaaa atatataatc ttgtagtaaa aagattttta gcagtactat
    11941 ctcctccttt tgaatatgag cagacaacta taagtgcaac tgtagaagga gaaatattta
    12001 aggctaaggg aaacaaagta ataaatttag gttggaaaga aagttataaa gatattgaag
    12061 atgaagatga gtatgaaagt ataccaaatg ttttagaaaa agatgtactt aaggtcaagg
    12121 gattaaagat aacttcagga aaaacaaatc caccaccata cttaaatgag ggaacattat
    12181 tgactgctat ggaaaagaat ggtctaggga cagttgctac aagagcagat ataatagaaa
    12241 aattattcaa ttcattttta atggaaaaga aaaataaaga tatatatata acatcaaaag
    12301 gaagacaatt attagattta gttccagtag acttaaagac accagaactt acagcttcat
    12361 gggaaaagaa attattggat atttcaaagg gaaaacttag taaaaataca ttcataaatg
    12421 acatgaaaga ttattcaaaa gatattgtac tagaagtaaa aaatagtgat aataaattta
    12481 aacatgataa cttaactaaa aatagatgtc cagaatgtgg taaatttatg ttagaggtaa
    12541 atggtaaaaa aggtaagatg ttgatttgtg aagatagaga gtgtaatact agaaagacta
    12601 tatcacaagt aaccaattca agatgtccta actgccataa aaaattagaa cttcgtggag
    12661 aaggtgaggg gagactcttc acttgtagtt gcggatacag agaaaagttg tcttctttca
    12721 acaagagaaa gcaagaagaa aaacagaagg catcaaaaaa agatgttaat caatatttaa
    12781 aaaatcaaaa taaaaatcaa gagacattta ataatccatt tgcagaagca ttagctaagc
    12841 taaaaaacaa ataattttaa agttattatt atacacaatt ataactttaa ctgtatatta
    12901 gttatatgta ctgatataat atagtgtata gtttaggcat gaaagtaggg ctgttaaatg
    12961 gatttaagtg gcatatttaa atactattgt aaagagtgtg aaaatacatg gaataattcg
    13021 agtgttgaat tatttgagaa tatagaaacg tatagtaaag attcacaaaa aaagagggaa
    13081 aaagaattag ataaattgct aaatacaata tcagttcatt tagagaggta tccaagtgat
    13141 gctgtattga gaaaaatgtg ggtaaaaaag ggcgaggttt tcttacaaaa gacattggaa
    13201 aaagaaaata tttttaagtt agaaaaaatg gatgtagagg atagaaaaaa atttttagat
    13261 ataacaaaac agtttattag agatgctaga aaatttgatg atgatttacc tataggtgat
    13321 attatgcaag ctatgaggaa tgtatggatt tcaaatgcat tacaattatt atttggtaaa
    13381 gaagtatatt attcaaaagc taactttgca tatagtatgt tatatccata tactgataat
    13441 tatttagaca atacaaatat agataaaaat gataagattt tatttaataa ctggttagaa
    13501 aaaaggctcc tgggagaaca cattaaatct aaggattatc atgaaagtaa agtatctcaa
    13561 atgatagatt atattgaaag tgtataccct agagaaaagt ttacagaagt ttatgaatcg
    13621 ttattattaa tatttaaaag tcaagtaaat agtttaaaac aacatggtaa ggaaaatcat
    13681 ttgtgtaaag aagatttatt atccatttct attgaaaaag gaggttcatc cgttttagta
    13741 gatggatatt taataagtgg attgatgaca aaggaagaaa tagagttttg tataggatat
    13801 ggatttttat tacaaatatc tgatgattta caagatataa aagaagattt aaaatacaac
    13861 cataaaacta ttattacaga gatgtcaaaa gagggtactt tagataaagt tgtaaataaa
    13921 ctaataaatt ttactattga gttaatagat agttttaaaa ttaataataa aaataaatct
    13981 gtaatagcta tgataaagaa tgattgctta atgttaattt tattttctgt agtttataat
    14041 gctgaatttt tttctgtagg atatataaaa gaagtagaga aatttattcc atatacaata
    14101 gattattcat tagagattga agaaaaaata aaagaaaaat ttaaaaatat agatgtttta
    14161 aataatgaaa atgaatataa agaaatgatt gatattattt gtgcagagta gaaaaaaagg
    14221 gagtgtctta gaatagactt tttagtttat gaggcactct ttttgtataa aatcaaatgt
    14281 attttcatca ttttaacaat taaaaagagc ttatccctat ttgagaaagc cctttttaat
    14341 ttaaataaaa atgtcaaggt taaactattt aaatgcagta ataatcccat tatcatgtaa
    14401 gttttttatg attgtagctg cgatagggaa tccaaataat ccagctattc caaatagttg
    14461 agctccaaca aacatacaca ttaatgttat taaaggatat aggccaattt ggtgtcctac
    14521 tacctttggt tctaatattt gtctaacaac ggttataatt atgtatagta ttaaaagtcc
    14581 tattgctaaa ttaatatttc cattaatgag tgagataatc atccaaggtg tcaaaatact
    14641 tcctgtaccc aagactggca aaatatcaac aagtgctatg atgacagata tagtaaatgc
    14701 attttcaacc tttaggatag taagtccaat aagtagttct atgaatgtca ctgatagtag
    14761 tattgcatag gcttttagta gttttaaaag agcattaata ctgctatttt ttatatttat
    14821 tatcataaat tgactttttt ctggaaattg ttttaagata aagtttacta ttttttgata
    14881 gtcaaaagta aagaaaaaag aagcaattaa agtaataata agttttaaca taaatgcagg
    14941 tgttttactt gcaatactag caatagcatt gagtgcattt gttgaaatag aagctactaa
    15001 agacatcata gattgactta tattatccca accaagtatt aatgctaggt taacattagg
    15061 tgtactagca tctatctttg aaaataaagt atttagcgtt ggttgtatac ttgatttata
    15121 tatttcagga agtctaaaaa atacatcact tacacttgca aatatcttca caccaaagct
    15181 aactaataaa aatatacaca acccataaaa aattaaaagt attactatag aaataagtgt
    15241 acgatttaaa tttgtattat ttgtaataag tcttataata ggtcttaaga tggatgcaat
    15301 gataaaactg attacaaaag gcattaataa tggcaataag aattttaata caatatatac
    15361 aatacttgtt attaaaacta catatgtaaa tttaattata aaattctttt gcttttgaat
    15421 atccataata taaaacctcc aatgtatttg atattattct aaatatggtc ttttttacag
    15481 aaaatatact actgtatatt gattataaac tataaagctt atgtaaagta aataaattat
    15541 gtaaaattta ggttaatttt gtataaagag atacttttat attttacaat taataaccaa
    15601 atactaaaaa atattttaaa taaagaactt aaaattgtga taaaattaat ttatagatat
    15661 taaaaaaagt ataatatatg gtttttaaga ggtgctaaga tgaaaaattt tagagattta
    15721 ggtgggaaca aaactgagga tggcagaact gtaaagaagg gattatttta taggtctgca
    15781 aaattatcaa atctaagtga aaatgatata aagattttaa aagagttaaa tattaaatat
    15841 atatttgatt atagaagtga tgaagaagct cgtaaacatc catcaacaat aatatcaaat
    15901 ataaaaaata taagaatacc agcaatgaga gagctagaag aatcaggagg aagttttggt
    15961 tctattgaag atatgattga tggattgttt gaaaaagatg gtgcatttaa tatgttaaat
    16021 aatagttact ataatttgcc tataaataat ccgtcatata aaaaattagt agaacttata
    16081 agagactatt ctaatttacc aatattaaat cattgtacag ctggtaagga tagaactggt
    16141 gtggggagtg ctataattct tatgatttta ggggtatcga gagagaatat aatgaaagat
    16201 tatctaaaaa gtaatgattt tgcagataaa gagatagaaa gatttataga atataagcct
    16261 aaatttaagg acattccaaa agaaaatttg aaatatatat ttggtgtaaa tgaagagtat
    16321 atgaaaactg catttagaag aattgatgaa gaatacatta gtgtagaagc atatttatat
    16381 ggagaattta atttaaataa agaagaaata agaaaattaa gaaatcaata tctagagtaa
    16441 aaggtgagtt tatgagttta aatataacag caagacagat aaatataata aaaatattgt
    16501 taaattcaaa agatacaatt agtggtattg ccttaagtca agagatagga tgttcttcaa
    16561 aaactataca aaatgaaata aaggatatta ataaacaact tagaaatggt aaaattttgt
    16621 caataagagg agttggttat aaactagaag gaagttttga tgaggtaaat ctaaatagca
    16681 atctctatga tgatgtagat agagtagaat atataatcag aaaaatatta accttaagta
    16741 atgaagaaaa aaatactata aaacttgaag atttagccga ttctatgtat gtaagtgttt
    16801 caacagtaaa aaatgattta aaagaagtaa aaagaatgtt ggaaaagtat tcaatatcag
    16861 ttaaatctaa gcataaacaa ggtatatgtg ttttagaaag tgaagataag atattaaatt
    16921 gtatagttga cttaagcaat aagagagata atcaattgag tttaaatgat tttttaaatg
    16981 atgatataaa ggaaagaaaa ctattaataa aaaaattatt attagacata ttaaatgaaa
    17041 atggattag
//
LOCUS       DAFKMX010000086        17026 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFKMX010000086 DAFKMX010000000
VERSION     DAFKMX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.086"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            217..999
                     /locus_tag="LZB08_002155"
     CDS             217..999
                     /locus_tag="LZB08_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBM7315653.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(1118..1771)
                     /locus_tag="LZB08_002156"
     CDS             complement(1118..1771)
                     /locus_tag="LZB08_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7315654.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(1795..3159)
                     /locus_tag="LZB08_002157"
     CDS             complement(1795..3159)
                     /locus_tag="LZB08_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBM7315655.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMLKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            3817..5247
                     /locus_tag="LZB08_002158"
     CDS             3817..5247
                     /locus_tag="LZB08_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315656.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            5350..6453
                     /locus_tag="LZB08_002159"
     CDS             5350..6453
                     /locus_tag="LZB08_002159"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBM7315657.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            6468..6704
                     /locus_tag="LZB08_002160"
     CDS             6468..6704
                     /locus_tag="LZB08_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7315658.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            6721..7770
                     /gene="vorB"
                     /locus_tag="LZB08_002161"
     CDS             6721..7770
                     /gene="vorB"
                     /locus_tag="LZB08_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBM7315659.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            7772..8512
                     /locus_tag="LZB08_002162"
     CDS             7772..8512
                     /locus_tag="LZB08_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBM7315660.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            8515..9054
                     /locus_tag="LZB08_002163"
     CDS             8515..9054
                     /locus_tag="LZB08_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBM7315661.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            9178..11277
                     /locus_tag="LZB08_002164"
     CDS             9178..11277
                     /locus_tag="LZB08_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBM7315662.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(11544..12059)
                     /locus_tag="LZB08_002165"
     CDS             complement(11544..12059)
                     /locus_tag="LZB08_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBM7315663.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(12151..13362)
                     /locus_tag="LZB08_002166"
     CDS             complement(12151..13362)
                     /locus_tag="LZB08_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBM7315664.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            14038..15480
                     /locus_tag="LZB08_002167"
     CDS             14038..15480
                     /locus_tag="LZB08_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBM7315665.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            15522..15875
                     /locus_tag="LZB08_002168"
     CDS             15522..15875
                     /locus_tag="LZB08_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315666.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKFYYKIKTFKN"
     gene            16066..16887
                     /locus_tag="LZB08_002169"
     CDS             16066..16887
                     /locus_tag="LZB08_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBM7315667.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         6461 a         2063 c         2939 g         5563 t
ORIGIN      
        1 agttccaatg tttgcagtaa tttattctat aattaaggat attgttgaaa gtaaattgga
       61 caaaaaggga ttaccgacag atacttccga ttatatgtag ctaaaaaata ttatgtataa
      121 ttaaaaatat ataaattaag caatatttaa agcactgaga aagttaaact cttggtgctt
      181 tttagatata aaattattat agaagaggtg ttttttgtgg catttataga atgtaaattt
      241 aaatctaaat atactggagg agaaactgat ataattgtaa ttttgcctgt agcaaatgga
      301 aaagaacttt ttagtggtaa agagatatat gattttgaag gtaaatttaa aacattatac
      361 ttatttcatg gtttagaaga tgaccaaagc tcatggatta gatatactaa tgtagagcat
      421 tatgctaaaa ataaaaatat agccctagta ataccaagag tagaaactag tttttatact
      481 gatatggcgt atggacacca atattttact tttgtaagcg aggaattacc taaatttgtt
      541 agagctatat ttcctttatc agataaaagg gaagataact ttgtagctgg tatgtctatg
      601 gggggatatg gagctttaaa attagcgctt tcaaaaccaa aagagttttc ggcagttgca
      661 agtttttctg gttcgcctga tatgattcat gagttagaaa atcaacaaat agaaaccaat
      721 gcagacattc tatttcacga ttttggtaga ccagaagacg ctagaaatag tgagaatgat
      781 ttactttaca tacttaagaa ccttaaagaa agaaatgaag atattcctaa aatttatcaa
      841 ttttgtggtg atagagattt tttatataaa aataatcaga tgtttaaaaa atatgcagat
      901 tctctgggtg tagatgttga atatgaagag tgtgtagggg ggcatgaatg gcgtctttgg
      961 aatttatgga taaagaaatt tatagatata attggatagt acacttatgg tattaacagg
     1021 tatactataa aatatcttta aatatgataa agactgaaat cctaaaatca aattatgatt
     1081 ttaaaatttc agtctctaag aatttaataa aattttatta agttcttaca cgagttatta
     1141 ttggagtaaa attgtcaact atatctatat agtatttact caattcctct aaaatctctt
     1201 cgcataaatc cgtactatga ccaagattaa taagaaactg ttttgtaaaa tgctttatta
     1261 cagcttgaga agatatatcg tacttgtgtg aagtataaac tctaattatt tcaggtttca
     1321 tagcaagtat accaatacaa gaaagtatta attcatcatg ttctatagat tctttatcat
     1381 taacatatct tttcatgacc atttcggtta cacttctttg atagcttaaa atagcttctt
     1441 cataagctat atccaaataa aaatttctat atgcttcgtt ttttagaagg aaacaaaggt
     1501 cagcattcat agcaagaaag aacatcatca tagtaccttc ttcatggtag ttatagatta
     1561 aagactgcat ttcatttctt atatcttcat atattatagc tcctatttcg cttttacttg
     1621 agaagtaata gcttattaga cctaaattta tattagctct ttctgcaatt tgcctagttg
     1681 ttgtcttatg ataaccttgc tcaaaaaata attctctagc agcacttata atactagctt
     1741 ttgtttcttt gcccttttct gattttgaca tatttactgt gtatacctct cttttcaatc
     1801 tttttttgaa aatttgctaa ctataaatat tgtaatagaa ccacctatta taggaaccaa
     1861 aattctgtca aatacagtaa gaataccaat catatttaat aatgccaata taacacttac
     1921 tataacagca tagatagctc ctttttttgt agcacctttc cagcaaagac caattacaaa
     1981 tggtgcaaat attacacctg tacataatgg agccggccaa aacaataatt ctaataaact
     2041 atcaaattta agtgctatga ttgcacctat tatagctgtc aaagctgatg caatttgaga
     2101 atactttaga gtatttttta aatcattaaa ttctatatta ggatgtaata atttgttata
     2161 aatatctctt gatataatag cactcattga tgtcattaat gaatcggcag tagacattat
     2221 ggcagccaat atagcagcta agaataatac agatattaaa ggtggcaaag tagtcgatat
     2281 catttgaggt aaaacctgaa cagatgatat gtttggaaat aaactaccac caatagctcc
     2341 aattattcct atagggaaag caagaaataa tagtataaat cctgcaatta cactactttt
     2401 aactgcattt ttagcattat cagcacattg atatcttaaa aaactttcat aagaaaatat
     2461 tgagcctaaa actaatggta aaactgtata cataaaatca ctaatcgatt tatttcctgg
     2521 aaagaatggt tcttttaaca ttgattgtaa cataagtaaa gggttgtcgc caactaattt
     2581 taagcttata taagctacta ttggcaaaaa cactatacaa atagcaagtt gaataaatgc
     2641 tgatactgca actccccaca ttcctgatgt ataagtatat ataatgataa ctataactcc
     2701 tataattaca gctatatcat aaggaatacc aaatgcagta gatacagaac caaaagaaat
     2761 caattgacta cccataatag ctagtataga tattggtgtt acaagacttt gataagctct
     2821 taaaaataca ctatgaccat atctagtttc aatataatca gcaggtgtta tatcacaaga
     2881 taagcttctt aattttcctg cgaaaaatgc atttataaag caagctaaag ctgcacctat
     2941 agtagaatac attccaaccc aaagtcctct ttcagctccc cattgaactc caccaactaa
     3001 gcaccctcct ccaaaagatg tagcagcaat ggttgcagct agtataggca aaggcagatt
     3061 tctacctgcg attaggaaat cggatgctgt tttaactttt tttgaaaaat aaaaaccaat
     3121 ccatatgtat attgcacacg taacaattaa aagtaacata gtcttttctc ctttataaaa
     3181 tattttaata aaatatacaa aatatattta aaatatattt tatataaaag tagtacgata
     3241 tttgttttat tggttttaga ttaatatagc tataataatt tatgatatta gatggaaatt
     3301 acgtaaaaat agaaaattga aaattctgaa atttttgcat attgatattt atctcataga
     3361 ttcttttttg taattgtaac acgcttttat ttttaaatca atagtacatg tataaaaata
     3421 aaaggaattt aaataagaaa aagatatttt taatatctgg atgtagtata gaaatatctt
     3481 gaataaatta ttcttgaata aaattataaa aaattaaaat cactagaaat atgataaaat
     3541 aatttaaaat acgcatttca atgacatttc aaaacaagaa aataataaaa aacaaaccta
     3601 aattaagtct aaatagcttt ttagttaatt ttagtagtaa aaaataaaac ttaaaaagtg
     3661 tttgacaatt atgaattttg agaatataat taaattaaaa ttatacacat ataaaaatat
     3721 actcctgaat caagaatgag atatttaaga ttcaaaatat atattattat acgtgaataa
     3781 taaaaagtaa gaaaatttta taagggaggg gtagatgtga gtagtcaaga aagcaaacca
     3841 aaaggaaatc ttatatcaaa ttttagagat gatatgagag accctaagaa aagagcaata
     3901 tacacatatt caagttctat aaacttagcg tacaatttta taggttactt tgtatcaact
     3961 gttacaaata tttttttaac tgatgtggca aaacttggtg taattatggc aggtggtatg
     4021 caaattatac aatctttcat aaaaattgct gtagctccta taacaggtac agtatttgac
     4081 aaacaaccat tcaaaaaagg aaagtattat ccatggctta gatatgcacc agcagcgtta
     4141 gcattaacat atggaatatt tttcatagtt ccattaatga aaataagtcc agcattactt
     4201 ttacctttga tgacagcttt attattaata gcatcttgtg ctcagcaagt agttttcact
     4261 ataatttatg ctatttaccc tgtagttgct aagacaccta gagaccgagt tataggctca
     4321 actactacga atatatttaa agaagttggt aaattcttag taggtttttc atatccgtta
     4381 ttattagttt tatttgcaga tgtattaggt ggagaaggaa tgggttattt aggtacctac
     4441 ttattctttg cagcaggatg ccttttagta ttttggtttt cttcaacaga gataataaaa
     4501 tctggagcag aagaagaagt tcttcaaaaa gaagagaagc caaagattaa agcaagtgaa
     4561 atggcaaaag ctctattcac taatgtacca cttatgatgg cattttgttt agaatttctt
     4621 atatgtataa gatctatagg attaggacct ttggctccat attattttaa atatgtaata
     4681 gaagatgaac gtggattagc agtattttta tcaatcatgc ctttggtaag tgtagcattc
     4741 atgtttttcg caccagtgtt tataaaaatc tgtagagagc aaaaactagc ttcaataatt
     4801 tctttttcta tatgtgcact atgccactta ttggttgcat ggactccttg ggggaaaact
     4861 actttaggag taactatgtt gttggcaatt ggtggaggtt tttcaaatgt agtatcaata
     4921 ataaatctta atttctttgc cggttcttgt gattatgggc attggaaatc aggaaaagac
     4981 ttacctggct taagtatgtc actatatcca gttgcaatac aagtaggagt attacttgca
     5041 actactataa gaacagtttt aatgaactca atgggatatc aagctgatat ggtagtcaca
     5101 gaagcagtta aatcaggttt tataaatatg atttcttatt ctatggcaat acctttaata
     5161 atagctgttg ttatagcaat actatatcca gtaagtgaca agaaattaaa ccaaataaga
     5221 gaagagttaa atcaaagaaa tgcttagtta aaagaaagtt aagtagatgg ttaatttaag
     5281 ctattaaagt aagtagacgc aatagcgttt atataaatga aaatacatat acagaggggg
     5341 cttttaaaaa tgatagaaaa attattgaga aataccgtaa aagaattaca tcaatatgtt
     5401 ccaggtgaac caatagaaaa agttaaggaa aaatatggtg taaaagaaat aataaaatta
     5461 gcttcaaatg aaaatcctct aggaccatca ccaaaagcaa tagaagctat gattgaaatg
     5521 ttaaaacaag gtcaacttta tccagagcca gaagctaacg aacttagaag aaaattagct
     5581 gaaaagttag atttaaaacc agaaaatttt attgttgcta atggtgcaga taacgttatc
     5641 actctaatag gtgaggcttt tataaataga ggagatgaag ttatatattg taacccaaca
     5701 tttccatcat atagaactgc aactataaaa aatgaaggga taccagtaga agtaccttta
     5761 acagaagatt ataagtatga tttacaaggt atattagata agattacaga taaaacaaaa
     5821 ttaatatgtg tatgtaaccc taataatcca acaggaacaa ttgtggatga taaagagtta
     5881 gaagaatttt taaagaaagt ccctgaaaat attataacta tattggatga agcatatata
     5941 gaatttttaa cagttcctaa ttatgtggat ggacttaagt atgtaaggga aaattataac
     6001 gttatagtaa caagaacttt ctctaaaata tatggtcttg cagggcttag agtaggatat
     6061 ggtattgcta aagatgagat aataagaact ttatttacag taaaagagcc tttctctgca
     6121 aatcgtgttg ctataagtgg agcaacagca gcacttgatg atgaagagtt tataaaagaa
     6181 acatatgaat taaatagagt tggaatggct tactttaaag aagagtttac aaaaatggga
     6241 tttgatgttg tagattcaca atcaaatttc ctttatgtag atatgaaaac agatatacct
     6301 aagttatttg aagacttgaa aaaaagagga tttgtaataa gaccaagtgc tacacatgca
     6361 cgtgtaagta ttggaactat ggaagaaaat aggaaatttg tagcagtttt aaaggaaata
     6421 ttaaatatta aaggagagcc aagtattgga taatagatag aaatattatg aaacggggtg
     6481 tagatatgaa cttaaaaata aacgcagaga gatgtaaaag ttgtgaatac tgcgttatat
     6541 cttgtaaaaa aggagcatta aaaatatcaa gtaagataaa taaagaaggt tatgctcatg
     6601 tagaagttga tgagagtaaa tgcatacttt gtggaatttg ctatcaggtg tgtccagata
     6661 atgtttttga aataattaaa gaagaagcat gtcaagaggc ttagaaggag ttgggataat
     6721 atgaaaaaga tgtggaaggg gaaccacgcc attgcagaag cagccctaag aggaggttgt
     6781 gaattttatg caggatatcc aattacacca caaacagagg taatggaatt tttatcacat
     6841 agaatgtctg aacttggaag aacttttata cagtccgaaa atgagatggc tgctatatat
     6901 atggtatatg gagcatatgc aagtggaatg aggtctatga cgagttcttc aggaccagga
     6961 atatctttaa aacaagaagg gatttcttac ttatgtgcaa atcattaccc ttgtgtaatc
     7021 gtaaatgtac agagatgggg gcctggtctt ggctcattgg actcagcaca gacagactat
     7081 ctaagagata ctagaggggg aggaaatgga gattatcatt tgatagttta tgcaccaaac
     7141 agtattcaag agactgtaga tttgatgtat aactcatttg atgtagcgga gaagtacaga
     7201 gtgcctgttg aaattttgac agaagcagct ttaggtcaaa tgatggaacc agtagaattt
     7261 ccagagttta agaagagaga aggagattta ggttggactt atgatggaag taatagagac
     7321 catgcaaagg ttgcagataa ccaaaagcca actttttgta tggagaaacg tatgagaata
     7381 actgaaaatg aacaacaatg ggaagattat caagtagaag atgcagaata tgtgtttgta
     7441 gcgttcggta taccatcaag aacaactatg aatgctgtta gaagacttag agaacaaggt
     7501 gaaaaagttg gaataataag accaataaca gtatggcctt tcccatataa agcatttgaa
     7561 aaggtaagta aagatgtaaa aggatttatc tcagtagaat caacagacac aggtcaatta
     7621 gtagaagatg ttgctttagc ctcaaagaaa gtatgtaaag aaaatgtacc tgtgtatggt
     7681 ttatttagtg gaaatcatat accaaaaaca ttgcaagtaa tggacactta tagcaaaata
     7741 aaatctggcg aaatcaagga ggtgttctaa tatggctaca gaaaaattaa cacctagcgt
     7801 tgtttcaata ccaaacaagt tttgcccagg ttgtggtcat ggaatagtaa atcgtataat
     7861 agcagaggta atagaagaac atggatatga aaaaaatcat gtattaacat taggagttgg
     7921 ttgtgtctgc aatatgaatt ttagctggaa tggtgataag atgcaaactg cacatggtag
     7981 agcttcatct acagctatag gagttaaagt agcacttcca gatacattag taatgactta
     8041 ccaaggtgat ggagatgctt atgttatagg tctttctgaa actttaaata cagcatatag
     8101 aaatcataat gtgactgtat ttgtaataaa caataataat tttgcaatga ctggtggaca
     8161 aatgtcatgg accactatgc cgggtcaagt cacaacaaca agtgtaaatg gaagagatat
     8221 aaagactact ggtagtccac tgaaagttcc tgagatggtg gcaaacttct ttgatgtagc
     8281 gtatgtagca agaggttcag ttcatagccc aaaggaaata attaatctaa aaaaatatat
     8341 aaaaaatgca atagaagctc aattaaatgg agagggatat tccttagttg aaatattagc
     8401 accttgccca actaactggg gtgtatcact agagaaatct atcaaatgga tggaagaaga
     8461 aatagtgcca tattatgctc ttggcgaatt taagcaaagg gatggtgaat agttatggaa
     8521 aaagagctag tatttgcagg gtttggtggt caaggagttt taactcttgg tctgattgta
     8581 gctgagtcag cacttgaatt ggggaaacaa gtaacttgga tgcctgcata tggtccaact
     8641 atgagaggtg gaaaagccta tagtgtagta aagttttctg atgagagtat aggtgggcct
     8701 gatatggaag aaatagatgt tttagtagca atgaataaac catctttaga ttatataaat
     8761 ttagttaaag aaggtggaac agtagttatt aatacatctg caatagatga gaatgtaaca
     8821 ttaagagaag atataagtgt agttaaaata aattgtcagg aattagctca aaaagtaaac
     8881 aatccaaaag cagcaaatat agtagtactt ggagcattaa ttgctaaaac tgggttattt
     8941 gaaaaagaat tagctttaaa aactatgtgt gatttctttg aggaaaaagg aaaaggaaag
     9001 tttaatgttc aaaatgaagc agcattcatg gaaggctata ataacgccat ttgataaatt
     9061 acccttatct taagtgattt atctcaagat ttaataatct ttagtgccaa gagaaacttc
     9121 atcttgagat aaaattcact taaaatcaca actataaaca tataggagcg tgaatttatg
     9181 agtttggaaa aacttattag accaaaaagt attgctatag taggagtgac tgataaactt
     9241 ggttttggaa gaagtgcagc acttagtata gtaaaaagta aggaaacaga tagagtatat
     9301 tatgttaatc ccaaaagaga agaattgttt gggagaaaat gttataagac gatacaagaa
     9361 gttccagaag tagtagactg tgtagtagta tgtacgccta gaaatgtagt tccatctgta
     9421 ttaaaagatt ctggagagtt aggaacaaag gcagcagtgg tatatgctag tggatttgca
     9481 gaggaaggta cagaggaagg tacagattta gaaaatcaac ttatagaaat atcaaataca
     9541 tacgatatga aaatattggg tccaaactgc atggggttgc taaattgtat agataaggtt
     9601 aatctatggg caggaggttc taaatgggat ttagatacta aaaaacctgg tataggaatt
     9661 gtaggtcaaa gtggatttat aactgctgaa attgtttcaa gtgattattt taatatatct
     9721 tatggttttt caacaggaaa tggaaatata gttacactag aagatgcagt agatttcctt
     9781 gtagatgatg attatgcatc agtaatagca atttaccttg aaggtttaaa aaatcctcaa
     9841 aaatttatag atgctttaaa aagggcagca caaaaaagaa agccagtaat aatattgaaa
     9901 tcaggaaggt cagaaaaagg tgctatttct gctgcttctc atacaggaaa tttagctggt
     9961 tcgagtaaag cttttgaaag tatttttgaa aaatatggag taatttctgt ggaaaattta
    10021 gaacagttta tgtgtttagc acaagctttt agtgttttag atggtaattt acctgcaaat
    10081 tctaattttg cagcaataaa cttctcagga ggagaaaata ctatttgtgc agatttagca
    10141 gaagaaaatg gtgtagaact tgcagaaata tcttctgaaa ctaaagaaga aatgaaaaaa
    10201 tatcttccag gatttgcaac acctaaaaat ccactagatg ctacaacagc attattccat
    10261 gaaaaggata tgatagtagg tttattacat acctttgaaa aggatgatag tgttggattt
    10321 actatggtag gtgcaaatat aagagatgaa gaaaatgaaa tgcatgaaac cctttgtaaa
    10381 gctgtaagtg aagctagaga acaaggattg aaaaaacctg tgtttgcagt tcctacactt
    10441 gaaggaaata gatatttaga ttataggact agattagaag ataatcaagt gcctataatg
    10501 tcatcagttg gaactacatt tacttgtttt aataaaatgg ctaagtttat agattatgat
    10561 tattcaaaaa gaactttaga atttaaagct gttaagaaaa gagagtcaaa taatgtagta
    10621 gcattatcag aattagatag taagattgag atgaaaaaat atggagtacc agttccagga
    10681 caaggtaatg caaaaagtat tgatgaatta gatgaaatct taaaatatat taagtatcct
    10741 gttgttttaa aaataaattc ttctgaaata ttgcacaaat ctgatgttgg aggagttaaa
    10801 ataggaatta agaatagaga tgaggcagta gatgcatata atgagatttt aactaatgta
    10861 aaaaaagcaa aaccagatgc taatattgat ggtatattag ttcaagaaat ggtagaaagt
    10921 ggtatagaaa ttattattgg tataactaat gatgaccaat ttggaccaat gttattagtc
    10981 ggtttaggag gagtatttgt agaggtattt aaagatacta cattatatcc attacctata
    11041 aatcatgatg aagctattat gatgcttaaa aaactaaaat catttaagct tttaaatggc
    11101 tatagaggaa gtgagccttg cgatattgat gctcttgcag atatgatggt caaattagga
    11161 aaatatgctt atgaaaataa ggatgaagtc aaggaaatag acttaaatcc agtgtttgta
    11221 tatcctaaag gaaaaggcgt atgtgcagta gatgccttaa tagttaaata taaatagttt
    11281 ataagttttg gtattcaacc ataattaaag caccgactga aaatcacatt ttgtaagtta
    11341 gatagtgtga ttttcagtca ccccaaattt aatttaaaaa tattttaaaa tgaaagtata
    11401 gttttaagtt tcattgtgaa atagcactgt ataaacaata aaatactacg tataaaacga
    11461 ataataaaag gtgttttaat gataagaatc aatcttgatt ttcattaaaa caccttcttt
    11521 attatattaa aaatttttaa acgttatatt gtcacaggtg ggtttgttgg agctgcatat
    11581 gtacgattag caaattcttg gtcaacaaag aatagaccac cctctccagc aagtttaaca
    11641 cgttttatat tgtcttcaca atttgtctct tcttctgctt gttcagatat aaaccaaagt
    11701 aagaattgag aagttctaaa gtctgattca gagtttgcta cagctgctaa gttgtttatt
    11761 aaagctgtaa cctttttttc atgagctaac gtacgctcaa gaatatccat tgcagaagta
    11821 aagtctgaat ctggcattgg gatagcatca agctgaacct ttcctcctac attatgtaag
    11881 tatttgtaaa aaaacatagc gtggtctgtc tcttctttat attgaacata aaaccaatta
    11941 gaaaatcctt taagtccttc tgcttcaagg taagaagaca tagaaagata aagatatgca
    12001 gaatataatt catggttaat ctgctcatta agtaattttt ccattttagc tgaaatcatt
    12061 tttatacctc caaattttaa atgctcttat aaaatataat aatattaaat ggaaaaatat
    12121 tcaataaaag tatttaaata ttttacatat ttaataaaaa tttatactac cattaaaaaa
    12181 attataactt acaatggcta tagagccaat taatggtgca tttgctccaa aatttgaatg
    12241 ccttacactt atttttttat atttagaaaa acttgctgaa gcagtcaatt tttttaataa
    12301 tgtattttct ataaatgtac caggtatatt aaagtcatag cctattatta tagaactaca
    12361 atcaatcaat gtaagtgtat taactaatgc atatgatacg tatgtacaaa attcatcaag
    12421 caaaaacatg gcaagactat ctttattatt tgcctcatct attaacttga ctaaagaaaa
    12481 atctttacag ttaattaatg gggagttagg ataaatatgg ctcaatgatt caattctttt
    12541 aattagatta ctgacacttg tataataatc taaacatcca ttgttaccac aactgcacaa
    12601 tggtccacta aaattaattg atgtatgtcc tatttctcca ctttgtccta ggttgccagt
    12661 atgaagttta ttttctagaa ctagaccagc tccaatacca ttcattatgt gtaggtatat
    12721 ataatttgat atatttttac ctaaaccata cattttttct gctaatgcac ccgaattagc
    12781 atcatttatc aaaaatattg gcaaatcaga catttctttt ataaaatcta ctatatttag
    12841 gtttgataca tttccaaaat ctgggggatt tagtatggta ccatttatat catctactgg
    12901 acctattgaa gaaataccaa ttccaattat atctctcttg ttgattttca ttattttagt
    12961 aaacattctt tttattatat cttttaaaac atcatttgat agacatttag ggtaagtttc
    13021 agaaagttgt tttacaatat taccttttaa atctgaaata attaccttac atatatttct
    13081 tcttattagg attccacaaa tacaaggcga tatattagaa atatctaata aaataggttt
    13141 acgacctgat gaatgtgcat ttaaagttga tgactctatt tccctaatca tattttcatt
    13201 aataagttca gtaactagat ttgataaagt cattttagta agtcctgtag acttagctaa
    13261 atcaactctg gacataggtg tatttgtaga aagtaatttc aatattatta acttattttt
    13321 aatttttaca tctttgatgt tagaaccttg tttgttactc atcataaatc tccttaaata
    13381 aagttaagtg ttttatttat tttcaagtat actatcacct tataatagag taacattttt
    13441 ttacttgaaa atatcattaa ttagaatata tcacattata tatcataaaa tatataaaaa
    13501 aatgttagaa ttaaattaaa agtgaaaagt agattgatat ataaatagta aatacttaat
    13561 taaaaaaaga taatgatagc aataattatt gttaccaaga atctactact tcaagtgtta
    13621 ccttaggtat ttaagttgat agtggtttgc acgtaataaa cttgatgtaa tatatattct
    13681 taataatatc gataaaagat ttacttaaaa ataatataga ttatgtaaac atataatcta
    13741 tttattgtat acaataaaat ttcattttgt aaaacagtga attaaatatg taatatagat
    13801 atcgattttt attacaaatt gttactaaat ataggtacat tagaacaaaa atctagtaaa
    13861 ttgtaactaa aatgtaaatt tttcctttaa tctatacaat attatggaaa ctttttgtaa
    13921 tttatgatat aataacaaat atataattat taaattataa taaatgacta tagcgtagat
    13981 gtaatactat gttatagtca tttttgaatt aaacttgata tatcaagggg gaagaatatg
    14041 agaacaatta gaagagtttt agcattaggt ttaacattag ctatatttct aataaatgta
    14101 cctaatgtag atgcattaac atcagataca attaaaggta acaatatata tgagacggct
    14161 ggattaatag cagataaaaa gagttatgat acagcaatta tggtaaatat ggataattct
    14221 atagcagatg gtctttcagc aagtggtctt gcaggtgctg ttgatgctcc aattttgctt
    14281 gcacaaaaaa ataagatacc aaatgaaaca aaacaaagat taaaaaatgt taaaaagata
    14341 tacataatag gtaaagaatt atcaataagt aagtcagtag aaactgaact aaaaaacaca
    14401 ggggcacagg taactagatt gggtggagat gacagaataa agactagtta tagtgttgct
    14461 aaagaggtaa atggtatcaa aaaagttgat gaagtgatat taactaatgc atataaagga
    14521 gaagcagata caataagtgc tgcacccgta tcagtaagag atatagctcc tatagtactt
    14581 acagatggaa agagtgtgcc tttttcaaca agcaatgtaa aaacatatgc agtaggtggg
    14641 agtatatcaa tgagcactag tttagttaat aaaactaatg ctaaaagact tggtggttca
    14701 gatagatatg atacaaataa gaaagttata aaagagtttt atccagatgc atcagaattt
    14761 tatttgagtg atggatatga tttagtaaat gcacttacag gttctacaat tgctaaggaa
    14821 aatccaattg tattggtgtc agaaagtagt gataagtcta tattagcagg agcagataag
    14881 attactagat taggttcaat aagtgacagt gtgtataata agtgtgtttc tgctgcacaa
    14941 aataatggcg attcgtctac aaaaggtgag tcacctatga aaaatgagac aagcatatta
    15001 ggtcagccaa cagctagttt agaagcgtgc ttaaaatggg caaaatctaa aaaagctaat
    15061 gatttattta tagagttaat accaatatta tatgatactg ctgttcagga aggtgttaat
    15121 cctgttttgg cagtagctca atctgcaaaa gaaactggtt tttgtaattt tggtggagta
    15181 ttagatgcat catttaagaa tccttgtgga cttaaaactt ctgtaggtgg ttctgatact
    15241 gataagaatg cccattcaag atttgatact tgggaagaag gaatattagc tcaaattcaa
    15301 catttgtgct tatatgcagg acaagatggt tacccacttt caaatccagt agaccctaga
    15361 catgaaaaat ctttatttgg taaggctaaa acagttgaaa gtttatctaa taattgggct
    15421 ggaggtcaat atggacaaga tttagtaaga atgatgggag aaattgaggc aacaaaataa
    15481 ttgaagatta tcaaatatcc aataaataag gattgattat tatgaaaaag agtaatattt
    15541 ttagtatcat atttcctaca gtcataatgg tgctaatgtt tttaactttt gctacagata
    15601 tatttaatat tccagatatt caatctaaaa gtatttttgt tgtgggaatg gttttgattt
    15661 ttccaatttc ttttttgata caagggataa tttgtgtatt aaataagact aattggattt
    15721 tatctcttat tgtgtcttta gtaacttata tagtattaat gtatatattt ttaaatgatt
    15781 ctgcatatat atatgtttta ctttatacag ctttttatat gattggacat ataacagcta
    15841 aattctatta caaaattaag acttttaaaa attaaattga agtatataaa agtaacatta
    15901 ggagaagtat ccttaatgat tttctaaaat cagaaatagg tgcttctctt ttttgtaaaa
    15961 ataaaagtat gtaaataaaa tttaaccatt ataattttct tttaacaaat gtatacaata
    16021 aacttgttgt ataattaaga tagataaaat aaagggaggg ataatatgaa tttaatagat
    16081 aatcatattc atactaattt ttcaagtgat ggaaaggatt ccatggaaga tacaataaaa
    16141 aaagctataa gtattggtgt tagatatttg acttttacag accatttaga acatgatgaa
    16201 gatagaggat tttgcataaa ttataatgac tatgttccag tatttaataa gtttaaagaa
    16261 aaatataaaa aggacataga gttattacta ggtgttgaag tgggttacag aaaacatcta
    16321 aaaaatgaaa tagaagaaat aataaattct aatccttttg attttgtatt gtgctcaact
    16381 catacaattg acaatgtacc tgttccatca aaggcgtatt ttaaaggtct tagcaaagaa
    16441 gatgcgtatt ataaatattt taatagcata cttgaaacta atcgtgaatt tggagattac
    16501 aatatatatg gtcatttgga ttatatatct aggtatggaa tatattcaga taatagagtt
    16561 atatacaatg actttaaaga tgttattgat gaggtcttaa agtcaataat caataatggg
    16621 agtggtatag agctaaatac ttctggttat agatatggat taaatgctat acatccaaat
    16681 gaagatattc taaaaagata taaagaactt ggaggattta tagttactgt aggttctgat
    16741 tctcataggg tagaagatat atgtaaagat tttgatgtgg cctatgatat gcttaagtat
    16801 cttgacttta aatatgtatc tttatttaaa gaaagagaaa cttattttgt aaatatagaa
    16861 aaagttaaat ctaacaatat agcttagagt gtgttttgaa gtaaattaat ggatagttaa
    16921 aaagttaata gttataatat tgtatcctct agaaaaatac tagaggtttt tattttgcta
    16981 aaataactaa atagaattaa atttaaatag tatagcttat tttaaa
//
LOCUS       DAFKMX010000087        16993 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFKMX010000087 DAFKMX010000000
VERSION     DAFKMX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.087"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            382..777
                     /locus_tag="LZB08_002170"
     CDS             382..777
                     /locus_tag="LZB08_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315668.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNNTCTTE"
     gene            820..2919
                     /locus_tag="LZB08_002171"
     CDS             820..2919
                     /locus_tag="LZB08_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBM7315669.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(3144..8354)
                     /locus_tag="LZB08_002172"
     CDS             complement(3144..8354)
                     /locus_tag="LZB08_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBM7315670.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMKFVLGKQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8369..10864)
                     /locus_tag="LZB08_002173"
     CDS             complement(8369..10864)
                     /locus_tag="LZB08_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7315671.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11437..11724)
                     /locus_tag="LZB08_002174"
     CDS             complement(11437..11724)
                     /locus_tag="LZB08_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315672.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11744..12019)
                     /locus_tag="LZB08_002175"
     CDS             complement(11744..12019)
                     /locus_tag="LZB08_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315673.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12656..12835)
                     /locus_tag="LZB08_002176"
     CDS             complement(12656..12835)
                     /locus_tag="LZB08_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315674.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(13120..14586)
                     /locus_tag="LZB08_002177"
     CDS             complement(13120..14586)
                     /locus_tag="LZB08_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7315675.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKCIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLNAMEFVTITLEDVVFLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLDIL"
     gene            15743..16921
                     /locus_tag="LZB08_002178"
     CDS             15743..16921
                     /locus_tag="LZB08_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7315676.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT         5749 a         2888 c         1875 g         6481 t
ORIGIN      
        1 gtattcggtt ataaatttat ataaattata taagctagat aaagtattac ctcaactata
       61 taagctagat atataaatta catatataaa ctagatatat aaattgcata tataagctag
      121 ataagaatta tttcaactat ataaactaca tatataaatt acatacataa gctagatata
      181 taaactgcat atataagcta gataagaatt atttcaacta tataaactac acatgtaaaa
      241 ttcatgtaaa tttactaata taaatcaaat aaaattacta aactctctaa actaattttg
      301 taaaacatac tctattttta acatttgtta ccttatacat aagtacaaac aaaatctaac
      361 taaatgaaag gataatgtaa aatgaatatt ttaaaaatga aaaaaacagg gatattttta
      421 ggaggagtac tttttggtac tgtaggtgtt aagatacttt caagtgattg tgttaaaaaa
      481 acttgcactc gtgcaacagc aggagcattg aaagttaaag attctgttct tgaaacaact
      541 acgaaaattc aagaaaatgt agaagatatt ttagctgaag caaaagaaat caacgagcaa
      601 gaagacttat ctacatctaa ggtaaacgtt gtacaagata ctcttaaatg cgaacaagaa
      661 gttcttaaaa ctgaaactat aaaaactgta gaagatacta attctaatag cattaaatgt
      721 gaagaaatca aagataacgt aattgaagaa aaaaataata cttgtacaac agaataaatt
      781 taatgattat tatgattaat aatatcagga ggaagtaaat tgaattttta tataaaacac
      841 gaaataagag gtcgtatacg ctttgactta caacttggca aattaaacaa caagcagtcc
      901 gatactctct tatattattt aacttctata aatggtatta ccaaagcaaa agtttacgat
      961 agaactggtg atgcagtcat ttattacact ggtgaaagag aatacataat aagagaaatc
     1021 actaaattca gttttaacaa tagcaaaata gaaaacctag ttcctgaaca taccagcagg
     1081 gaattaaaca attattatag agacaaatta gcaaataaac tgctagtcaa aagtatctcc
     1141 aaaatatttt ttccaaagtc cttaagattt tttttaattg gacttcattc tataaaatat
     1201 tttaaaaaag gtctctcttc cctgctaaat aaaaaaatag aagtttctgt tttagatgcc
     1261 actgctatag gtatttctat ttttagaaaa gatatcaata cggctggttc agttatgttt
     1321 cttcttggta tcggtgaact attagaagaa tggactagaa aaaaatctat agacgacttg
     1381 gctcaaagta tgtcactaaa cattgaaaaa gtatggttaa gaaaaggtga cactgaaatc
     1441 ttaattccaa tttcagagat aaaggaagga gatttagtaa gtgtaactat gggaaatatg
     1501 attccattgg atggagttat agtatcagga gaaacaatgg tcaatcaggc atcacttaca
     1561 ggtgaatccc ttgcagttaa caaaaaagaa ggctcttaca tatatgctgg aactgtaata
     1621 gagcaaggca atattgttat gtgcgtcaag gaaaaagctg gtactactag attccagaaa
     1681 atagtaacta tgattgagga atctgaaaaa ctaaaatctt ctgtagagag taaatttgaa
     1741 catctagcag atacattagt tccatacagc tttttaggaa gtatattaac ctatgcaatt
     1801 actagaaacc ctataaaatc actctctata ttaatggtag atttctcttg tgcattaaaa
     1861 ctctctatcc ctatttcagt actatcagcc atgagagaat gtaacaataa caacattact
     1921 gtaaaaggtg gaaaatttct tgaaggtgtt gcatgtgcag atactattgt atttgataag
     1981 acaggcacat taacaaaagc tcaaccaact gtttcagata taattacatt ccaaaattat
     2041 aataaggagg atatgttaag acttgcagct tgcttagaag aacacttccc acactccata
     2101 gcaaatgcag tagtttatga agctgaaaaa caagggctta gtcacaaaga aatgcataca
     2161 gaagtagaat atattattgc tcatggcatt tcaacaaaag ttgatgataa aaaggttgta
     2221 ataggtagca aacattttgt atttgaagat gagaagtgta ctatcccaga cggtgaggag
     2281 gataagtaca ataatctatc agatgagtat tcacacctat ttatggcaat atcaggtaaa
     2341 ttatcagcag ttatttgcat aaatgaccct ttacgaaaag aagcaaaata tgtaatttca
     2401 aatttaagag aatgcggaat aaaaaagatt gtgatgatga ctggagatag cgaaaaaaca
     2461 gctaaatcta tagcaagtaa agttggtgtt gatgaatatt attcagaagt cttaccagaa
     2521 gacaaagcca attttgttaa aagtgaaaaa ctcaaaggtc gcaaagtaat tatgattggt
     2581 gatggaatca atgattctcc tgctatttca gaatcagatg tcggaattgc aatgagtgaa
     2641 ggtgctgaaa tagcaagaga gatttcagat attaccatat cagcagataa tttgaataat
     2701 ttaatcgttt taaagcagat tagtaataag cttatgaaaa gagttgattt aagttctaag
     2761 tttattatcg gctttaattt aggtttgatt ttacttggtg taggtggatt tgttagacca
     2821 agtacatctg cctttttaca caacgcatct actgtcggaa taagtttaaa cagtatgaca
     2881 aatttattgg aaaacaccaa tacttataac taccattaat ctccattaca taaaacatat
     2941 gatataaaaa atttataatt catcttttaa aaagaatatg tcttaaaatt agcttttttc
     3001 taatcaaaat tatataaagc atactttaca aattttaaac tcaacttatt tctatttttt
     3061 acaaaaaagg agatgattca aattttcatt ttgaaacatc cccttttgta aatttcttta
     3121 aaatttatat attaattata ttttcaacca ataattgtat tttcaaccaa tctttcttca
     3181 tagcatttta aatcattttg ctttagataa ttaaattcag actctacata aataccacag
     3241 cctaccaatt caattgtcaa attaggtata ttttccttta gtatactagt ctgctcataa
     3301 ataattcttt ccaataaatc aacttgtaat atagaaaaat gcttcataat ctccccatca
     3361 ctatctatat ctttcttgat ttcataccaa ctctttgaca aaattccctt caaatcaata
     3421 taccctagac tcctcaatat atctatacta tcgctccatg catacttact aggtttatca
     3481 aagtatgatt ctagtttctt cttctgctca tttgtaagtg aatcataaga aagcttccta
     3541 ttaaaatgtg ccttcaaaga aatcttagcc atagaatatg gacttctacc agagtttaat
     3601 cctgcatttt tgaacttttc aattccttta tcttctactg aagaccatat tgagtttatt
     3661 gaatctgaat cattgtattt aacatcaaga ccatgtgtaa tatttatata gttttgtgga
     3721 aatacatatt gataatagac atctttttta tcatcatata gagagaagta tccactatca
     3781 tataaatgaa tatgcacctt tacttttaaa ctatcctcta aaatatttcg tctaaggttc
     3841 ggtatatttg catacatggt tacatatttt atggcatcaa agtttttatt taatggaact
     3901 tcaaaactaa tttcatctcc tgtcaaaagc tttttcactt cttcttcatt acaatcatat
     3961 agagaatatg tattttcaaa tcccacacgt gatttcccat ctaaactgcc tgttaatgct
     4021 accttcaaaa tatggttatc tgttttttgc cattggtaag catcttggtc aagaaactct
     4081 ctaatctgtc tattttgctc tttcgcctga tttataacct cgttttcatc ttctgtaatc
     4141 cctacatggc atctatttaa gatatttact atattgcttc caaatggaaa tgtccatagt
     4201 tgaaaagctg ctgcctcaac ctgacacaaa tatctaacct gatttaattc aatttgatgg
     4261 ataagttttt tctgatattc attcatctga ttaccactat ctgacatttg ataaatcaat
     4321 tctcccattg caagattatc ttttttctta tcaatcttat cagtaatgat aatttgagtt
     4381 tccatcatat cctgtacttt tttaaatatt atagaaatct cctctgaatt ttccattttt
     4441 aagcttccaa acttcgtgct taattcagtt aaaacttcct tcatctttaa tttacttatc
     4501 tcattttcaa aaatatcttg atttttaact gtactatcga atttttctac aaatggtata
     4561 acagattctt tcaaagtttt atctttaaat tccaccattt gagtattcaa ttcatctaat
     4621 ttcttaatat tattttcttt atctttaatt gcctctttaa ctacctctat tttactctca
     4681 aaactagatt ttacattaag acaattatta acaccactaa tcgcagtatt tataagagaa
     4741 caagcagaac ttccaatact caatgcattt ttaattttag atgtcttttg ctgtctttca
     4801 atagcttgtt tacttttctc tagtgcagct aacttatctt tttgctgttt attattgtaa
     4861 tttgcctcca acatacctat ttcttttttc tggatttttg tgtaaatttc cttatactcg
     4921 ctttcactta acccctcttt tgctctttga atatcgtcag ctttgtctgc atagttatca
     4981 tcatctatag acttaactat aatctcctta atttggtcaa atttagttct tctttcttca
     5041 tcaacacctg ccaaattata agtcatttta tctaaagcat tttttctatc ttcacttagc
     5101 tgttttccaa gtacaaattt cataccatca gaatccaatt taaaatctga aaaattaacc
     5161 atcctgttag tccatttgta ttcctcttct ttagctgaaa gattttcctt caaagatttt
     5221 gcactgtcaa atgcccctag acaagcttga ccaacaccac taaatgcagt cagtaaactc
     5281 aatgcgcttt gtattccaga aagaattaac tgtgttttca tttgttcctc aagttttgcc
     5341 tgtttctttt ttagttcctc tatgtcttct tgttcatctt ctttcatctt tataatttct
     5401 tgaatcaaca ggttcatttt tgcattaaca gaaagcattt tattctcaag ttcatttttt
     5461 aaaacctcta tttgttgatt tccagattca atttgagtta ggatactttc cttatatgta
     5521 ttgaaatatc cttcttttaa ctcatctttc ttcccttgtt ccaattctct taaggtctta
     5581 tccagataat ctgtcaaaga acgaatatct gtaacacgat ttcctccaat ttcactttct
     5641 tcaatcatct gcacataaaa tgctgtcttc tgctcaagtg caaataaaat catactccat
     5701 tcgctaaaat tctgattttt tcctacaaaa gattctattc tatcagactg catcaattta
     5761 tttataaggt attcatagat ttcctttgct tctttataaa attctttctt tgtattcata
     5821 tcatccagag aaagtactgc tttatttttc ttctcaaaac tatagtactt ctgaagtatt
     5881 tctctattta attgcatctc tattttctca actgcatgaa aaatttcata aatatgtgaa
     5941 agttctaaaa tatcagcttc taaaattcct actgcacaca acagtctaag atttctataa
     6001 cctttaattc cagaaaaacc aaatctctcg aaaaattcat agaagaatgt atctattgac
     6061 aaagaatact cctgctttaa caattccttt tcttttagtg catctccaga gtgaccggct
     6121 tttccctctg ctccggctga acctgaatct tcacgtttgc taattgtcca gtccttatgt
     6181 tcttcataaa gagtatttaa tttctctcca tcatctgctg cttgcttctg atttaaagca
     6241 tgattaaaca catatctatc aaaataatag tagtttctat cactattatc cattggagga
     6301 gaggtaccta tatatcttat tgtttcttca tatatacctc taattccacc aagacctcct
     6361 gcaccaccag ctcctccaga gcctcctttt cctgctaaac catgatttcc atctttttta
     6421 cttatagcac tttttaataa aggagctttt ccaaggagat aaacctgtcc agacttgctt
     6481 ccaagacctg catttccacc attacctcct gtgcctccag ttccaccttg ttttcctttt
     6541 tctgctttta ccacatagta ttttgtacct ttaacattag caaattcttt tgactctttt
     6601 ttagaaccta ttttagcatt tgccccagct tctccagtta tccctgtatc tccttttttt
     6661 ccatcttgtc catttccacc acgtccacca tctacgtaaa gtttaagttc tcctcttttt
     6721 tctatttctt taaaagataa tatgatatca cctgaagtac ctgcattatt gccatttctt
     6781 ccattcattc cgtcgtaaat agaactttct ccaaccaatt tttctgcatc cttaccagat
     6841 acgttgattg aataagtccc ttctggtata tgtaattttt ctgtaagtag tacaatattc
     6901 ttacctgaga agacttcttt ctccatatta cagtcaattt tcaactcaca atctccatac
     6961 atatatatag atgacaaaat attaagttga taaccagata ttattttagt caaatccgat
     7021 aaattcactt taggtatagg aatatatata gaaagtacat tttctttctc atatgatttt
     7081 atatatgtac aactttgaat cttctctatc ctatactctg catcttcaag ctttttggct
     7141 tcttcttccc tatattcttc ttctgctatc caatatttga tatctttcaa tgtatcttct
     7201 aaagtatttt ttagcaatga ttttaacaac tcacttagac caacaatatg agtctcacta
     7261 cagtaactat ttaactctaa aagaagacgt gtttcttcta atatattcac tttctgtacg
     7321 ccatcaccat atgaaagttc ttgtacaaac tccataagct ctttcatatt attagtccta
     7381 ctacataaat ctgtatacat cttatctaat tgaatctttt tatcttttag actatttata
     7441 gaagatagaa tggaaataga atcttccata ccttttttaa cactttctat ataaggaact
     7501 aaaagttttt ctaggtcttt ataggttaca tcaacagctt tttgtatttc tattttacaa
     7561 gaagcagtaa gcatcaatcc caaatctgtt ttttcaaatg tttcaggtag atttttcatt
     7621 tcattcaaca acttttctgg ctgtttatct agtggattac ataatttaat agatacacca
     7681 tctgataatg ctacatttaa taagtacact aattccttac aaccgtcgta atctttctct
     7741 tgactatcta ggtcttcttt tgctttttcc aatatatttc ttattttttc ttctgttaca
     7801 ttaccaatta gtgttttagg tattttattg ataagaaaga agaaattttc tttatacttt
     7861 gcataatcac caagcatatc tttcaaattt ttgcctgttt ctatcaataa tgtatgtcta
     7921 gagtccagta gggattgata gtctaaaata actaccatcc cttttacctt tgaagatgtg
     7981 gaaatagttt cccttacaag catagcacca gcaatcatat actctgctcc acggttgtca
     8041 aaaaaacctg ggcaatcaca taatgaaaaa tctctatttg caatatctac aacttctgga
     8101 cataaagtct tagaatatac accatttcca atttcgacag ctgaatcttt tgcagttata
     8161 tatatttgtc cagtctcttc atccgttttt tcttccatca cacagccttt taaataattt
     8221 atacaagtac tctttccaac gcctgaagca cctaggaata acaaaattga cttttctttt
     8281 aagtcatatt ttctaagctc gtctcttcct tcttccaaaa ggtttacgat ttcactataa
     8341 agtttatttg ccatacatcc atccctttct atatttcgga acaatagcct tgatattttt
     8401 ctttaataag attctcttct acctttgaaa cagctgttga taacttagta tttggagcat
     8461 taaatcctcc attgtctcct ctatctccat attcagcgta ttctcttgct ggtcctgttt
     8521 taccttcatt ccagcttcca ccactaaatt ctatatgata tttaattata ctatctaact
     8581 tataatccca acttgtaaaa tatcctgtat agtatacagt cttatctata ccacttggag
     8641 aaaatgaaag tgtattatat ccgtgatatc caccacttcc tccatatcca cctaaacctg
     8701 gtctaccatt tggacctgct tctccatctt tcactgttat atcacaatga ttaattattc
     8761 tatttttttg ttcctctgaa tctgaaatga agtaaatatt acctttctta ccagcatttc
     8821 cacctcttcc agcatttcca ccagctccac ctttttcacc ttttttgcca cttgtacctt
     8881 tttcaaaata ccctttaaac atctgtccgt ggaaataact tgtatatcct gcatcagctc
     8941 cattacttcc agaagctcct gtttcaccat ttttcccatc tattccttta gaaccatttc
     9001 ctccatttgc aatcagtgtt agacgataat ttctaatttc ttctttaata tataagtaaa
     9061 tattacctgc atctctacca tcttcaccat ttttcccata catagaatta tctacaagtt
     9121 ctccgctctt tccacttaca ttaattttta cattactacc tacatcaaga ttgttagaag
     9181 caagaataat atttttctct gatgcaaagt ccattccaag gttatcatcc atataaatag
     9241 tatctttagc aaagaacata aatgtagaaa cattgtcatc tacttttcta tttttgaaat
     9301 attccaatac actgctaatt actaagtttg gaattaatgc tcttatttca atacctttct
     9361 tatcattaat attaattgtt tttactataa atggatttaa aatgtattta ccaataaatt
     9421 ctctaattat ctcattacct tcttctccat atgcacttaa tcttgttata ctgtcttcta
     9481 actcttcatt ttgtgtaact gtaacatttg tcaaaattga attaaaatca cttacactat
     9541 atacataatc ctttttattt gcaacttcca ttaatttttc tctatttggt aatgtaaata
     9601 cctgtttttg cacttcataa gaaactaaaa ctttttctaa attctttctt attataatga
     9661 agttttgttc cattatcttc ttacttgcct tttctataat aaattccata tgtacagaag
     9721 gtaataaaat ttccccagtt ccatgttctg tatatttact taaagcatca attatatcta
     9781 agtttccttt tatcttattt tgtaatttca tgcaactatc ttgtaagaat aagccacttt
     9841 gtttggctaa tacgttgata aaactctcta tagtaagact tgttgtactt tccaaccagt
     9901 ttttaaatac ttttacaacc tcatcatatt ttgctatttc ttgaatcatt gttatacttc
     9961 caataatatt gataaaatct tctgtatacg ccttaacata atcttctagg ctagatacga
    10021 tattgttttg aatttctttt atcaacgtat ctaaaactaa tcttgcatct gcactaattg
    10081 gatattggaa tgctgatgtc ttatctttaa cttcatctaa ttctagtact tgctttaata
    10141 agccttccct ctcatctgat gcaagtggct tacaaagatg aatatttttt ccattatctt
    10201 ttagcaattc ttcaaataac caagcttttt cctctatatt actgtctaaa ttttcgcata
    10261 attgttcata ttttcttttt ataacattat atatttgttt ttctgttctt cctaagaaac
    10321 tttttgaaat gaaaaaagtt atactaccct taaaattttc tttatgaaga cacatattta
    10381 actgttggaa caggtctagt acttttgtaa gtctagaatc taatatatct gcttctggta
    10441 tcaatacaat tactgcacat atagatgcag aatttttaaa tacttcacct aatgaaactg
    10501 ctgcactaat atcatgggct actcccctat tgtcgctaaa acctgcagta tcacataatt
    10561 ttgcatccat tccttctatt ggttttctca tatttacaat gttcgggtat aaagtttgag
    10621 aaacagctcc tgaaccaatc tctgccacag ggtctaagca atcaatatat gatataccag
    10681 tatcttcatc ttctacttcc atcattacac atcctgcaag atagttaatt gcagtacttt
    10741 tgccacatcc tgtcccacca agaattagaa ttaaattttg attttcagcc ttatttattg
    10801 ctacttttcc ttcatttaat aagagtgtta tttcttctaa aagttgattg ttattttctg
    10861 gcatttattt tcccccttgg ttatatattt tatttaaata aagtttaatc ccttaagtag
    10921 attacaaagt ttttagacta tatttaaatt tgttttttat tttttatttt taataaattt
    10981 gctttttcac tcaataatta ttcttctttg tttattaaaa taactgtgtt gtataatata
    11041 atctttacca acacagttat tttatttaat gcgcttattt tattgtattt tatgtaatac
    11101 agttgttttt tatattatat gtccattatt ttcctatggt tattacatat agatttttaa
    11161 ttatatttgt accattaatt taaacattcc tatactactg cttaagtttt acacctatac
    11221 cagcttgaat attttatttt tatttgctta aatagttcaa ctatactggt ttaaatagat
    11281 tatctttatt tgcttaaata gttcaactat actggtttaa ataggttatc tttatttgct
    11341 taaattagtt caactatact ggtttaaata ggtttccttt agtttaaata gttcgcctat
    11401 acctacttga atagttcact tttatttgct taaattctat accaatactg gcttaaacat
    11461 tatactacca tgattgtatt gtctatcttc aactacaata aaaacaggtt ctaaatcttt
    11521 agtagatgtt atttttaatt ctaaaaaaac attatcagtt ggtaatattt ttacatcata
    11581 attatttaaa ttattattcg tgatactatt aaatacatca ccttgacgtt caaaaaaaac
    11641 attatcaact attacaaaat aaacatcttc ttttctacta ttttcaacct ctatagtatt
    11701 ttgtttagtt acatttagtc tcatcttcca cctcctatta atattactta attatattaa
    11761 tacttgtaat tgttgcatta tgttcatcac aaggaagagg tttaaaaaca actatctctg
    11821 ctaaataatt ctgtggagga tttgtaactg aagtagcatt gcaaataaat ttaaaatttg
    11881 ttcctgctac aacttgagta gaaacaataa gtggttcata accaacccct tctaacattc
    11941 ctacagcttc attaaatact tttaaatctt cttcttcgac agctctgaat tttgaccatc
    12001 caccaacaaa aagactcata taaaaacctc ctttatatat atatttattt atatatatag
    12061 tagtaccaat tttctcaata ttcaatattt tctgtgtaac tggtcaatga aaatgcataa
    12121 ctggataaaa atacttataa aataacttaa ctttacatat aaatagctta ctttacatgt
    12181 aataagatta ttctacaata tgaaatgcat cttgttttga atatattcta aatacaataa
    12241 tggatatttt caattattat aattagaaaa tatccattac ttaacttgtt ttatttaaaa
    12301 tcacatagag actttattat tttttttatt gcatgaatag ctatttcagg tatcataagg
    12361 tgatataccc cctccttgaa accacaaaaa acatgacaaa ctttacgaat cttggcttaa
    12421 aagaccacag gtacattcct tatttgtgga ttggagtagc cataacaagc aaattttaca
    12481 atttttatga tgacaaaaat caataaaatc gcttagtata taacacgaaa cactatttgt
    12541 tactattaag aatagttaga taggaagtat gcattataca aataaaggaa tcaataaaga
    12601 aagttaaaac atccctacaa attaaaaatc tgttacattt ttctatattt aaacgttatt
    12661 tacttacaat tggagaaata acaggttttc cttctcggtc aagtaaaggc gtaaatccgg
    12721 ctgcattgcc ataatagtga aatacatagt tcacacctgt ttctttatcc acccaaatct
    12781 ccgttgcatt tatttttcct tgtgaatata ctttttcaaa tctatctttt cccattttat
    12841 aatcccccat atttttgatt ggtctatacc ttcttcactt aagtcgaaaa aatacataac
    12901 ttattttttt aattctaata tgagcacgta aaaatgtcaa acagcttatt taatttcata
    12961 acttcatgtt actgttagta accttgtttg tttacttaca ctacattaca gcaccatttt
    13021 atcctttaca ttattgtatt gcacacagta aaaatgctta ctttatggga tttttcaact
    13081 aaaaaaagga ttttttctcc acagaacatt tactattttt tataatatat ctaattttaa
    13141 tctcatatca acatttaatg aagatataac ctttttcaaa tattgtaaag actccttaaa
    13201 aatattagaa aaagattctg caaatctctc aacatctcct ttttttaaat acctaagtat
    13261 ctcaaaacat acatcaaaac gtgttttcaa aatttcatta tattcttcat gaaaaaatct
    13321 ctctaaatag attccaaata tcatatattg ttttaaatgc aaaaatgttt cctgtaatac
    13381 ataaatttgt gtataatcta ctatcttaca aagtaaaaca aaatctggat aaaccttaca
    13441 ctcttcacaa ttattttgta agtaccttaa atgagtttct attttatcta cagctatcaa
    13501 atccatttta tttcctagta aaaatactac atcctcaaga gtaatcgtaa caaactccat
    13561 agcattcaaa agcaaactca accttttagc tccttcttct gtatttatat aatcatcatc
    13621 atattcagaa aaaagtgtta catatgttcc cttgccatta attgttttag caattcccaa
    13681 ttcatttagc tcattatagg cacttctaac tgttggaaga gatacattat atctattaat
    13741 tatcgaagta attgatggta gacaatctcc tattttaata ttgccttcaa aaatttgaca
    13801 aattacatct gaaacaatta agtcatataa ataacatttt tccaacgtta cagaattaaa
    13861 actacttgta tctatacttt gtggagcacg attttctacc attttaagta tggattctct
    13921 acaagcttta tacatatatg ttaatagttt tttaaattca attagattcc cgtccaatcc
    13981 atataagtaa gtttttctta cctgctctat gcatttatta cgaaaaaagt ctatacaaca
    14041 attatccaag ttatcgaaat tataggccaa ttgtgaaata atgacatcat gctgaataat
    14101 ctcacaaaaa tggtgaatca ggaaattatt tgttttactt attagtttat ttatatattt
    14161 tagcctttgt tctttaaaga ctaaataatt agaactattt ttttccatac aatcgataat
    14221 ctcgtttaat tcttcaaagt ctttagatgt aaatctttta atattgtaca tagcaataga
    14281 aggaaatatt aaactaaaaa tcttatatat ttctgatatt tgtaaaatct tctcatataa
    14341 taagtcagat tctattgatt gtttactcgt actctggatt aaaactggtc tagaaccagc
    14401 ttttgtctct ataaagccct tttctgagag catttttatt acagttttta tagtattttt
    14461 tgataccata tattcttctg acatgctttt tatagaatca agcctatctc caatagtaat
    14521 tttaccagta taaatttgtt gaattatatg ctcaaaaata attaaatatt gtggttttct
    14581 cttcatattt ttcctcttcg ataaattaga tttattgttt tttgtaaggt atatttactt
    14641 ttatttaata gggcaaaata gttgcttatt ttttcctcat aatctataat taatataaac
    14701 catttatata tatggtctaa gttttataat actgtaatgt agtaaaatag catgttgtag
    14761 ctatactacg gcaaattaac ataccattgt agtatattaa ttggaataat tttaagtact
    14821 tcaaaattcc atcaaaacat atatgtgttt ttgatattat gtatattcgt taatagaata
    14881 gctatacttg ttttacataa taaattatat gatacttgct atatgtaatt actatattta
    14941 atacacataa ctattatact tgctatatgt aattactata tttaatacac ataattatta
    15001 tacttactat atataattac tatacttgga catatagtta tcatacatac tatatgtaat
    15061 tactatactt atacacataa ctattatatt tgctatatgt aattgtacta tacttggaca
    15121 tatagttatc atacatacta tatgtaatta ctatacttat acacatagtt atcatacatg
    15181 ctatatgtaa ttactatact tagacacata actactatat ttactatatg taattatact
    15241 atacgtggac atatagttat catacatact atatgtaatt actatacttg gacacataat
    15301 tattatattt gctatgtgta attatactat acttggacat atagttatca tacatactat
    15361 atataattac tatacttaga cacattatca tacatactat atgtaattac tatacttaga
    15421 cacatagtta tcatatatac tatatataat tactatactt gaacacatag ttatcataca
    15481 tgctatatgt aattactata cttatacaca taactattat actttccata cataattact
    15541 atatttggac acatagttat catacatact gtatattaag atataatact ttttaaattt
    15601 tatataacac agacacatgc aataaaaaag aaggtatcca taataagctc tttgcatgta
    15661 cctaaataaa actgtaaaat agaatatata aaatctaatc tattttaagt aaatacaatt
    15721 tcactttaaa agaggatgta ctatgaaata ccatatattt ttaatcataa ctactatatt
    15781 aataatcgaa ctatgtaaca tttacatatg caaaatagca tcttttggaa acaaaaatat
    15841 aactatacat aagaattaca aatatatttt tttaataaaa ccattaacct tcttgataat
    15901 aatattcaca aaattaccat taccactata ttatatattg ttttacatag taagtcataa
    15961 attgctctta agcagaaaaa gttctactaa caaccatttt attcccaata tggcagtaat
    16021 aagtttttgt attttacacc ttattactgt aagtacaata tcacttgtta aaaatacaag
    16081 tccagatata atatttgcaa atatgaccta taagttaatt agtattttaa tagcttctat
    16141 tttagatttt atcttatggt actctatgaa taaaataaaa gagcatattt atataataac
    16201 tcaaaatcaa atagagttca gtctattttt aaaatttatc tggttcactt taggatatat
    16261 attatttgat agtatacaaa ttacatttgc tccaccaagt tctctaactt cctactttat
    16321 attgggaagt aacatattat taatcttaca actaattata ttcatcaatc atatatattc
    16381 cattgaaaaa aatatttatt tagaaaaaga acataataga ctaaaaatga taaaacaaga
    16441 acaattacta aaagtaaatt tattaaagaa aaaagctaac attgatgaat taacatcagc
    16501 atataatcga aaatttgctt ttgaaaaaat acagcattac attgataaca atttattatt
    16561 ttcattgata tatatcgatt tggataaatt aaagacgata aatgatacat atggacattt
    16621 agctggggat aactatatta ttaacttctc aagtatagtt attgaaaact taggaataga
    16681 tgacatattt gctagaatag gaggagatga gtttatagtt attatgccta aatctaatga
    16741 aaacaccaca aaagaattta tagagaaaat aagtaaaatt atggataata agatatcttt
    16801 tagctatggt attgtagaag tttctagcga aaacaaaatg actgtagaag aaattattaa
    16861 tattgctgat attagaatgt acactaataa acaaaataat cataaaagga acaataacta
    16921 aaatgaatat ttacatgagc ttacttgtaa tcttactatg ccttttatct tattctatct
    16981 atcattataa ttt
//
LOCUS       DAFKMX010000088        16517 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFKMX010000088 DAFKMX010000000
VERSION     DAFKMX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.088"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..183
                     /locus_tag="LZB08_002179"
     CDS             <1..183
                     /locus_tag="LZB08_002179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315677.1"
                     /translation="LHMEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNE
                     AFSILEKELNISKANT"
     gene            357..1487
                     /locus_tag="LZB08_002180"
     CDS             357..1487
                     /locus_tag="LZB08_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBM7315678.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1728..2078
                     /locus_tag="LZB08_002181"
     CDS             1728..2078
                     /locus_tag="LZB08_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBM7315679.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2086..2535
                     /locus_tag="LZB08_002182"
     CDS             2086..2535
                     /locus_tag="LZB08_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBM7315680.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2637..3212
                     /locus_tag="LZB08_002183"
     CDS             2637..3212
                     /locus_tag="LZB08_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBM7315681.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3205..4614
                     /locus_tag="LZB08_002184"
     CDS             3205..4614
                     /locus_tag="LZB08_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBM7315682.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4798..6231
                     /gene="eutA"
                     /locus_tag="LZB08_002185"
     CDS             4798..6231
                     /gene="eutA"
                     /locus_tag="LZB08_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBM7315683.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6249..7613
                     /locus_tag="LZB08_002186"
     CDS             6249..7613
                     /locus_tag="LZB08_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBM7315684.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7626..8507
                     /gene="eutC"
                     /locus_tag="LZB08_002187"
     CDS             7626..8507
                     /gene="eutC"
                     /locus_tag="LZB08_002187"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBM7315685.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVEKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8527..9180
                     /gene="eutL"
                     /locus_tag="LZB08_002188"
     CDS             8527..9180
                     /gene="eutL"
                     /locus_tag="LZB08_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBM7315686.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9191..9892
                     /locus_tag="LZB08_002189"
     CDS             9191..9892
                     /locus_tag="LZB08_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBM7315687.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKFANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDK"
     gene            9895..11364
                     /locus_tag="LZB08_002190"
     CDS             9895..11364
                     /locus_tag="LZB08_002190"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBM7315688.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11460..11747
                     /gene="eutM"
                     /locus_tag="LZB08_002191"
     CDS             11460..11747
                     /gene="eutM"
                     /locus_tag="LZB08_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBM7315689.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11874..12635
                     /locus_tag="LZB08_002192"
     CDS             11874..12635
                     /locus_tag="LZB08_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBM7315690.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEIVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12648..13277
                     /locus_tag="LZB08_002193"
     CDS             12648..13277
                     /locus_tag="LZB08_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBM7315691.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13319..14044
                     /locus_tag="LZB08_002194"
     CDS             13319..14044
                     /locus_tag="LZB08_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBM7315692.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14057..14329
                     /locus_tag="LZB08_002195"
     CDS             14057..14329
                     /locus_tag="LZB08_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBM7315693.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14322..14873
                     /locus_tag="LZB08_002196"
     CDS             14322..14873
                     /locus_tag="LZB08_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBM7315694.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14885..15976
                     /gene="eutH"
                     /locus_tag="LZB08_002197"
     CDS             14885..15976
                     /gene="eutH"
                     /locus_tag="LZB08_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBM7315695.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            15978..16451
                     /locus_tag="LZB08_002198"
     CDS             15978..16451
                     /locus_tag="LZB08_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBM7315696.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         6816 a         1859 c         3089 g         4753 t
ORIGIN      
        1 ttgcatatgg aaatcaatgt tatagaaatt ttccctaaag ataaagctaa acttaataaa
       61 atagaaatgg ataaagctag ttggtttgta aatataataa gtaaaaaata tcctaaagaa
      121 gctttaaatg aagcatttag tattttagaa aaagaattaa atataagtaa agctaataca
      181 taagctttga ttattgattt tatatactat tggttttata cgctatgact tgacattata
      241 ttaatcaaat agtatatttt taaatatagc acaatgaagt gttaaaagag caatggagct
      301 ccatagataa agtctatgga gcttttttgc gtatttaact taaggaggat aggattttga
      361 ataactttga gattaaaact agaataaaat ttggagaagg ctcactaaaa gctttgcaag
      421 aattaaaaaa caaaaatgta ctaataataa cagacccttt tatggtgaaa tcaaaaacaa
      481 tagataaaat aacatctaat ttaataaact caacatatca agtttttgaa gaaatagtac
      541 cagacccacc aatagaagta gtagtagcag gaatagaagc atttaaatct ataaatccag
      601 acgtaataat agcattaggt ggtggttctg caatagatgc agcaaaatct ataatggact
      661 tttctaaaaa aatattaaaa attaacgaag tagagtttat agcaatacca acaacaagtg
      721 gaacaggttc agaagtaaca tcatttgctg taataacaga tagtcaaaaa aaatgtaagt
      781 atccattagt gagtgatgat ttacttccag atatggcaat actagaccca gagcttgtaa
      841 aaactgtacc aaattttata acagcagata cagggatgga tgtattgaca catgctatag
      901 aagcttacgt atctacaaat tctaatgact tttcagatgc actagctgaa aaagctataa
      961 agttagtatt taaatactta ataaaagcat ataaagatgg aaatgacata gaagctcgag
     1021 aaaaaatgca caatgcatct tgtttagcag gtatggcatt taaccaagca tcacttggca
     1081 taaatcattc tattgcacat gtattaggtg gaaaattcca tataccacat ggaagaacaa
     1141 actctatatt acttcctcat gtaatagaat ataattcatc aatagtagga tatactaaca
     1201 caacttactc tgatacagct aaaaaatatg cacaaatagc agaattagta ggacttggga
     1261 gtgctaatat aagaatatct gttagaaatt tagtaaatga aataaagaaa ctacaaaaag
     1321 aaatgaatat gccaactaaa ttgacacaat gcaaattaag tttagaagat ataactaatg
     1381 aagaaagaga aatagcaaaa cttgctataa aagatacttg tacattaact aatcctaagg
     1441 taccaactga agatgatata ataaacatat taaagaacat taaataacta cattttatta
     1501 catgtaaata taatattata caaataggga aaatgttttt aaaaatataa attatatatt
     1561 gacaaatata aaacataaaa ctataatgaa agtatcacaa aggcgtgata ctttagtaga
     1621 aagcaatgga gcttcttagg aatattaata ttattcttat ggagctcctt ttattttaat
     1681 caaatagctt tttaataaac tatattcaaa gaaagcaggg aataaatttg actgaagaat
     1741 ctaagcaaag agttattcaa gaatatgttc ctggaaaaca ggttacatta gcacatatca
     1801 tagctaaccc taatgaagat atatataaga agttaggatt agtacttgat aaaaaagatg
     1861 caataggaat acttacaata acaccaagtg aagcgtcaat aatagcagca gatgttgcta
     1921 caaaggcttc aaatgtatct ttaggtttta tagatagatt tagtggttca gtagtaatat
     1981 caggtgatgt aagttctgta gaatcagcgt taaatgatgt tttagaagtc ttaggaaata
     2041 tgttaaactt ctcatctaca aaaatcacaa ggacataata atacaatgaa gaatataata
     2101 tttatgggaa agacaggtag tggtaaaact accttatgtc aaaaactaga ccagttagaa
     2161 ttaaagtata aaaaaactca atcagtggaa ctttataata atgcaataga cactcctggt
     2221 gagtatatgg aaaatagaaa cctatataat gccttaataa caacatcagc agatgctaaa
     2281 attatagcaa tagtgtatga tgccacacaa gaagaaaatt atatagcacc aggttttgcc
     2341 agtatgtttt gcaaagacgt aataggaatt ataactaaaa taaataaagc tactgaaaac
     2401 gaagtcaata tagcttatga aagactatca ctagcaggag caagtcaaat ctttaaaata
     2461 gacacagttg ataatattgg acttgaaaaa ttatttaaat atctagaaaa atataaaaat
     2521 atagatgaag tttaagggga aatatttttt tattcaaaaa tattattaaa gtatgataaa
     2581 ataaaaaaat gtataaacta ttttaagaag atttaatctt taagcgaggt aggaacatgg
     2641 ggaaaaaaat actattggtt gatgatgaac cactgttaag gatggataca aaagatatat
     2701 tagaaagtca tggctatgaa ataataggcg aagcaagtga tggatttgaa gctgttgaaa
     2761 tgtgtaaaaa acacaaacct gatttagtta taatggatat agagatgcca atacttgatg
     2821 gtataaaggc aggcaaaata atatgtaaag aaaatctagc tggtggagta cttttattaa
     2881 cttcatttga agataaagaa tacgtagaaa aggctaaaag tataggtgct tatggatatt
     2941 tagtaaaaac agcaagtgaa aaatcactta tcccaaccat agaaatgtgt ttaaataagg
     3001 taaatgaatt tgaaaaaatg aaaaaagatt tagaaaaagt aaattctaaa ttgaatgaaa
     3061 gaaaattagt agaaagagca aaaggtatac ttataagaga gtttaagatt tctgaggatg
     3121 aagcatacac tagaataaga aaattaagta tggataaaag aactacaatg atggaaattg
     3181 ccaaaatgat tataatagga tacgatgact aatacgataa aaaaaatatc aactgcacaa
     3241 acatacttaa ataataacga aattgaaaaa attatagaag tatcaaaatc cttagattta
     3301 atggcttctt tttatgaagc tgatgttttt atagatgtac taagtaaatt tgatgatgaa
     3361 gcaattgttg tggcacatgg tagacctaaa gaaaagtcaa tgtactgtga aaatgttata
     3421 ggtaaaaagg ctttaattga aaatgaacca ggagttataa agtgcttaaa aacaggagaa
     3481 agagtaagag atattaaagc tttaacacag gaatataaac tggtcaaaca aaatattcag
     3541 ccaataacac tagatgacaa agttttagca gtactaatag ttgaaaaaga tataagtagt
     3601 gaagttaaga atgagtttaa tgctagtgaa gacgaagaaa ataaaaattt acatcagttt
     3661 ataaaactta tcggaaataa tgacttttta gttgataatt taaattcagc catattaata
     3721 tttgataaaa atggaaaatt aaaaattaaa aatcgtattg caactgagat atacaaaaat
     3781 cttgggtttg gatatgatat acaagatatg cattacaatg atttagatat agaaaccaac
     3841 acctttgaaa atatattaat ggatgaaaat tatgatgaga caaatgaagt ttcaataggt
     3901 aatatgtttt ttaaaataaa gaccatatac atcaaagatg atgaatttaa ggtttgcaaa
     3961 atagtacaag atataagtga tttaaagaga aaagaagaag aattagtttt gaaaacagtg
     4021 gctataagag aagctcatca tagagtaaaa aataatcttc atacagtaat atctataata
     4081 aaaaaacaaa gtagattatc tcaaaatgat gaagtaaagc agtgtttaga taacataata
     4141 aatagggtat ttgcaatact ttcgactcat tatttactat ctaaagaagt tgataataat
     4201 atatctatac gagaaggtat caatttatta gtgtcaaata ttcaaggtgg ctatttattt
     4261 agtaaggata tagatatacg tataactggt gaagatttta aaattagtgg agaaaaagca
     4321 acagcaatac ttttggttat aaatgaaata ttacaaaatt gttatgacca tgcattttcg
     4381 gatagagaac atggtactat acaaatatta ataaataaag ataacgacta tagaaatatt
     4441 gctattatag atgatggaat tggatttgat agtaataata taaataaaaa tagcttaggg
     4501 acatatataa tagatagtta tataacacag atgttaaaag gaaatataga gagaaaaagt
     4561 agtgagaatg gtacagaagt attaattact atacctacac aaattaatat ttaaataata
     4621 actcatctac aaagtagtag ttttaattga gcaaagaggc ttaaggttat taatagatta
     4681 agttaattga atgtatttat aaaaattata agtatatatt caatttaaca ggtatttatt
     4741 ataactttaa gcctctttgc ttgtttaaga catacttaaa ttttaggagg tgagaaaatg
     4801 agagaagagt tattaagtgt tggtattgac attggaacaa gtactactca gttaattttt
     4861 tctaaattaa tagtagaaaa tactgaatca aatttttcgg tacctagaat aagtatagta
     4921 gacaaagaaa ttatttataa aagcaaagtt tatttcaccc ctcttatttc aaataatgaa
     4981 ataaatggtc aacaaataaa agatatagtt gaaaatgaat ataaaaaagc aaatatatct
     5041 aaagaagata tacatacagg agcagtaata ataactggtg aaacagctag aaaagaaaat
     5101 gcaaatgatg tacttcatac tttgagtgga tttgctgggg acttcgttgt tgcaacagca
     5161 ggacctgatt tagaaagtat aatatcagga aaaggagctg gagctcatat ttattcaaaa
     5221 gaacattcaa ctacaattgt aaatatagac ataggtgggg gaactagtaa catagcctca
     5281 tttaaaaggg gagaagtaac cgatactggg tgtttggata taggtggaag gcttgtaaag
     5341 atagataaaa taagtaaaaa aataacttat atatcaccta aaatagataa aatcataaaa
     5401 gataataatt taaatcttca agtaggtaca atggctactg tagaaaatac aaagccaata
     5461 gtggatataa tggttgactt attaatggaa agcataggat taaagaaaca gacacctttg
     5521 tttgatttta taataaccaa taaatcaata agtatagatg aaaatattaa aaatgtttca
     5581 ttctctggtg gagtagctga ttatatttac tatgatggag aaataaaaga ttattttgaa
     5641 tatggtgact tgggaatttt acttgggcaa gcaataaaaa attgtgaata ccttaaaaaa
     5701 gtaaatgtag ttaaatctat ggaaacaata agagccacag tggttggagc aggttctcat
     5761 actactgaag taagtggaag tacaataact tacactagcg aaaagtttcc acttaagaac
     5821 ttgccagttt taaaattatc aattgatgaa gaaacaggca cttcaaaaca aatcagtgaa
     5881 gctataaaaa ataaacttaa ttggtttaaa ctagaaaatg atttacagaa agttgctata
     5941 gccatgatag gaaagaaaaa tccaaccttt ttagatatac aagagtatgc taaagggttt
     6001 gtagatggaa tgcaacaaat catagaaaat gatttacctt ttatagtaat tgttgaaaat
     6061 gatatggcaa aagtgttagg tcaagcaata tatgcattat taaattatga aaaagaaatt
     6121 gtttgtatag acagtgtcaa ggttgaaaat ggagactata tcgacattgg aaaacctata
     6181 gttgaaggta aagttttacc tgtggtaata aaaacattag tttttaatta atagaaggag
     6241 gtaagtaaat gattttaaat acaaaattat ttggacgtac ttatcagttt aaaggcttat
     6301 atgatgtaat ggctaaagct aatgaagaaa aatcaggaga taaattagca ggtcttgcag
     6361 cagaatcagc tgaagaaaga atagcagcaa aagttgtttt atcacacata aaattggagg
     6421 atataagaaa taatcctgca gtggcatatg aagaagatga agtaacaaga ataatacaag
     6481 atgctgtaaa tgaaaaagtt tatgaagaaa taaaaaattg gacagtctct gaacttagag
     6541 aatggctatt ggattcaaat actacaagtc ttgatataag aagaatatca agaggattaa
     6601 cttctgaaat gatagcagca gttgcaaaat taatgtcaaa tatggactta gtttatgcag
     6661 ctaaaaagat aaaggttact gctcactgta atacaactat aggagaacaa ggaacattat
     6721 cagcaagact tcaacctaat catccaacag acgacccaga tggaatactt gcatcacttc
     6781 ttgaaggttt aacttttggt ataggagatg cagtacttgg attaaatcca gttgatgact
     6841 ctgttgagag tgttactaaa gtattaaaaa gatttgaaga agtaaaacaa aaatatcaaa
     6901 taccaactca aacatgtgta ttggcacacg ttactacaca aatggaagca ataagaaatg
     6961 gtgctcctgc agatttaata ttccaatcta tagcaggttc tgaaaaagga aatgaagcat
     7021 ttggatttaa cggcgacact atagaagaag ctagacagtt agctttaaaa cacggtacag
     7081 ctacaggacc taacgttatg tattttgaaa ctggacaagg ttcagaatta tcttcagaag
     7141 cacatcatgg aatagaccaa gttactatgg aagctagatg ctatggattt gctaaaaagt
     7201 atgaaccatt cttagtaaat acagttgttg ggtttatagg acctgagtac ttatatgatg
     7261 ctagagaagt tgtaagagca ggacttgaag accactttat gggtaaacta catggcttac
     7321 caatgggtgt tgatgtttgt tacactaacc atatgaaggc tgaccaaaat gatatggaaa
     7381 acttagccat actattaaca acagcaggat gtacgtattt tatgggaata cctcatggag
     7441 atgatgttat gcttaactat caaacaacag gataccatga aacagcatca cttagagaaa
     7501 tgtttggact aacagcaata aaagaatttg atgcttggat ggaaaagatg ggattctcta
     7561 aagatggtaa gttaacagag ttggcgggag acgcatctgt attattagga taggaggtat
     7621 ttaatatgaa tgagaaggat ttaaaagcat tggtagaaca attagttgga caaatggttg
     7681 gagaattaga tactaatgtt gtatctgaga ctgttgaaaa ggcaactgaa gttgtagtag
     7741 acaacaatgc ttgtatagat gatataacag aagttgatat aagaaagcaa ttactagtaa
     7801 aaaatcctaa ggatgcagaa gcttatttag atatgaaagc aaaaacacct gcaagattag
     7861 gtataggaag agctggtact agatataaaa cagaaacagt tttaagattt agagcagacc
     7921 atgctgctgc acaagatgct gtattctctt atgtagatga agaatttata aaagaaaata
     7981 atatgtttgc agtggaaact ttatgtaaag ataaagatga atacttaaca agaccagatt
     8041 tgggaagaaa attttctcca gaaactataa ataatataaa atctaaattt ggtacaaatc
     8101 aaaaggtttt aatattagtt ggagatgggc ttagctcagc tgcaatagag gcaaatttaa
     8161 aagactgtgt gccagctata aaacagggtc taaaaatgta tggtatagat tcaagtgaaa
     8221 ttttatttgt taaacattgt agagttggtg ctatggacca cttaggtgaa gaattaggtt
     8281 gtgaagttat atgtatgttg gttggagaaa gaccaggatt agttactgct gaatcaatgt
     8341 cagcatatat agcttataag ccatatatag gaatggcaga agcaaaaaga acagttatat
     8401 caaatattca caaaggtgga acaactgcag ttgaagcagg tgctcatata gctgaactta
     8461 taaaaactat gttagataaa aaagcttcag gaatagacct taaataaagt cgagggagtg
     8521 tagaatatga aaaatgatgt aattcgtcct aatatactag gaattaaaat aatttcaaat
     8581 ataagtccag aaatggcaca aaaactagaa ttaaaatcac atcacaaaag cttaggttta
     8641 ataactgcag actgtgatga tgttacgtat acagctcttg acgaagcaac taaagcttca
     8701 gaagttgatg ttgtatatgc aaaatcaatg tatgcaggtg ctgcaaatgc ttcaacaaaa
     8761 cttgctggtg aagtattagg aataatagca ggtccaagcc cagcagaagt aagaagtggt
     8821 ctaaatgcag tagttgattt tctagagtat ggagcaactt ttataagtgc aaatgatgat
     8881 gattcaatag catactatgc tcactgcgta tcaagaacag gtacatacct ttcagaggta
     8941 gcaggaataa gagaaggtga agcattagct tatttagtag caccaccact tgaagcaatg
     9001 tatgcactag atgcagcaat gaaagctgca gatgttaaga tgtgtgaatt atttgcacct
     9061 ccaactgaaa caaactttgg tggagcatta cttacaggtt ctcaatcggc ttgtaaagca
     9121 gcatgtgatg catttgcaga agcagtaaaa tcagttgcag acaatccaac aggattttaa
     9181 ggatagttag atggatgcta taggcttaat agaagtaata ggatatgttg ctgcaataga
     9241 agcttctgat gcttgtttga aatttgcaaa tgttaatatt gtaagagttg ataaggttgg
     9301 ggcaggtatt gttactttaa caatatgtgg agatgtcggt gctgtaaaag cagctttaga
     9361 agcaggtgaa atagcagctt ccagagttgg tacattaaga acatcacata ttataccgag
     9421 aatccataat gaagtaacaa atgctttatt caaaactaaa gaacctaaag tctgtgaagt
     9481 ttcagaagat aaagaaaaaa actttgagat tacattggaa atagctgata atgtatcgaa
     9541 aaatttatca gaaaaagaaa atgtaatttt agatgtcaat gaaaatagtg gagatactga
     9601 aaatttatat agtgatatag ataaaacaaa caaaaaaatt gaaacagttg atgatagttt
     9661 aacagaagct aatgaggaaa aaatagaaaa cactaatgat aaagaagtat tggaacaagt
     9721 taaaatagaa aattccgatt ttgatagaga tgacctatca aagaacagtg taaaaaaact
     9781 taaagctatg gctaagaaat tagattcttc aataacttat aaagacttaa atgttcttaa
     9841 aaaagaagaa ttaataaact tagttaaaaa gcttagtaga agggataaat agttatgaca
     9901 cttttagata aagatttagt atctatacaa gaagtaagaa ctcttgtaag aagatctaaa
     9961 gtggctcaag agaaactttc taaaatgagt caagagcaaa tagataaaat agtaaaagct
    10021 gttgtagatg cagcatttaa taatgctact aaattagcta aaatggcaaa tgaagaaact
    10081 ggatttggaa aatgggaaga taaagtaatt aaaaatactt ttgcatctat gggagtttat
    10141 gaaactttaa agaacacaaa aactattgga ataattggtg aagataaaga agccaatacg
    10201 atagatatag ctacaccagt tggagttgtt gcaggtctta taccttcaac aaacccaaca
    10261 tcaacagtaa tatacaaaac tataatatct ctaaaagcag gaaatactat agttttctct
    10321 ccacatccaa atgcaaaaaa ctgcatactt gaaacagtta atataataag tgatgctgct
    10381 ataaaggctg gcgctccaga ggattcaata ttatgtataa ctaatccaag tatagttgga
    10441 accaatgaat tgatgaaaca taaagataca aacttaatac tagcaactgg aggagaagca
    10501 atggttagag ctgcttactc ttcaggaaca ccagctattg gagttggacc aggaaatgga
    10561 ccagcattta tagataaatc agcaaatgtt aaattagcaa tcaaaagaat aatagattct
    10621 aaaacttttg ataatggtgt tatatgtgca tcagaacaat ctatagtaat agaaaaatct
    10681 atggaagaaa ctgttttaaa agagcttaaa aatcaaggtg catatgtttt aaatgaagag
    10741 gaaagtgaaa gattagcaaa attcatatta agagctaatg gtactatgaa tccacaaata
    10801 gttggtaaaa gtgttgaggt aatagcaaat ttagcagact taacaaatgt acctaaaaat
    10861 acaaaagttc tagttgcaag agaaaataaa gttggtcaca atgtagccta ttcaagagaa
    10921 aaacttacac caatactagc gttctatgtt gaagaaaatg tagaagctat tatgaacaga
    10981 tgtagagaaa tattgttaaa tgaaggtgca ggtcatacat tctcaatgca tgtaaatgat
    11041 gaagaattgg taaaaagatt tgcccttaat atgccagtat caagaatact tgtgaatact
    11101 ccttcagcac ttggaggaat aggtgcatca acaaaccttg taccagcact aacacttggt
    11161 tgtggtgcaa taggtggaag ttcaacttca aacaatatag gaccacttga tttaataaat
    11221 gttaaaagag tagcttatgg aatcaaagaa atagaagaac ttggaagaga taaatctcaa
    11281 gttgaaaaat caatgtttga ctcaatggat aaagaagaat taataaatct tcttgttcaa
    11341 aaactagtca aagaactagc ataactaaaa taaacaatga aacttatagc tatcgctata
    11401 aatttctaaa aatataaact ataaaaaact ataaaattaa atttttggag gataagatta
    11461 tggcaagtgc aaacgcatta ggaatgatag aaactaaagg attagtagga gcaatagaag
    11521 cagcagatgc aatggttaaa gctgcaaatg tacaattagt aggaaaagag caagtaggtg
    11581 gaggattagt tactgtaatg gtaagaggag atgttggagc tgttaaagct gctacagatg
    11641 caggagctgc agctgccgag agagttggag aactaatatc agttcatgtt ataccaagac
    11701 cacactttga agtagatgct atattaccaa aggtatcagc tgagtaatat aagaataatt
    11761 ataaaattta aattttgatt gaaaaagagg ctatctcaaa aatgagtctt actaataaac
    11821 tcaatgtatt ttgaggtagt cttttttata catctaatag gagatgtgat aaaatgggtg
    11881 ttttaacaga aaacgaagta aaaagaaaaa ttggaaaaaa caaaaataat ataactgaat
    11941 tttatataga aaaaggtcaa ataataactc cttctgcaaa atcttattta gctgagagaa
    12001 gtattgagct taaatatgtt gataaacttt cagatgtaaa caatgaacct aaagaaattg
    12061 taaaagaagt agttaaagag aaaaaatata gatatactac tgtatttggc gctaaattgg
    12121 atgaaaaacc agaacatatg actcaattaa atggaaatct tttagttttt aaagaccata
    12181 aaagaataat attgagagga aaaatagatt ctcttgaatc agaaatacta aaggtacaga
    12241 tactttgtca gaaactagat atgccaaagc ttgttaagga attgcaagaa gtattagact
    12301 ttgtaagaaa tataatgaga tgtgaagttt tagatgaaaa gctaggggac tttaaacttt
    12361 taggattaac tcctcaagaa cttagagaac aatctcacta tcctaaaaaa tactttggta
    12421 taggacatga gttcccaagt tatgaaatgg gagaagtagt agtagctata aatagtataa
    12481 gaagtaatac acgagaggtt gaacttgcag cctatgaagc atttaagggt gagtatggaa
    12541 atgttcacag agaagattta ataaaggcac ttaatagatt atcgtcagta ttttggataa
    12601 tgatatataa ggttagaact aataagtata agtagttgga ggacataatg gagaatctat
    12661 taaatgaaat agtggaagaa gtaataacta gagttaaaaa agaagcattt atagaagttg
    12721 aagcatcagg aagacacgtt cacttaagta gagaagatgt cgataaatta tttggagaag
    12781 gatatactct tacaaaacta aaagacctat ctcaaccagg tcaatatgca tgtaaggaaa
    12841 gagttacaat aactggtcct aaaggaagta taaaaaatgt agttgtactt ggaccatgta
    12901 gaaatgaaac tcaggtagaa atatctttaa cagatggttc aactcttgga ctaaaagctc
    12961 ctataaaaca aagtggagac ttagaaggta ctttaagtat taaaatatca acacaatatg
    13021 gtgaagttga acttgataag gggctaatgg ttgctaaaag acatatacac atgactccta
    13081 aagatgctga aaaattcaat gtatgtgata aagagatagt tgaagctaag gtgatgggtc
    13141 aaagaccttt aacatttgat gatgtagtaa taagagtgag tgacagtttt aaaacttata
    13201 tgcatataga ctatgatgaa gctaatgcat gtggttattc aaaaggaact gttgctaaaa
    13261 taataaagaa agcataaatt taacctacta tataaattac ctctagggag gatttaatat
    13321 gaattatgat aaccttgtag atttaataac ggaagaaatc tataaaaaaa taaatagtaa
    13381 tgaaataaaa atatctaata aacctaaagc tgtgattgta tttgaacaag ataacaataa
    13441 atttaactta ttaaaagatg aatttgaaat agtagaattt gataaaagta taagggaatg
    13501 tgaaatagtg atagtttcaa gactttgtat gagggggcta tcgaatatag cacttggaaa
    13561 ttcaacaagt gatgaagaaa gatttatact aaaaatgatt atgaagggca aaaaagtata
    13621 tgtcctagat gaaggaattg aatacaaaaa atataaggat actgcaccaa aagcattata
    13681 taagaaattt atgtcatttg aagatgaaat atgtaaattt ggtgtagaaa ttataaaaga
    13741 cttaaatgtt ataactaaaa ataagttaaa tataaagaaa gaactagaaa gtactagagg
    13801 ttctaaaaaa gaagatacaa aagttttaga taaagatgtt ttaaatttaa ccagtgtaga
    13861 aagtgtaaaa atagataatg aatttagttt agatttaagg aataaaaaac ttatatcgga
    13921 agctgactta agaaaaccaa ctataaatgg agttaaaaac atattagtta ataaaaaaag
    13981 tataataaca ccattagctg tagatttcat gagaatacac catctcaaac taaagaaact
    14041 gtaaggaggt aattttatgg atataggaaa agttgtaggt aatgtttggg ctacaagaaa
    14101 agatgaaaaa ttatgtggcc aaaaactttt agttgtaaaa attttaaaaa ctaaagacac
    14161 atacaaggaa ggcttcatgg ttgcagccga taatgcagga gcaggaaatg gagacttagt
    14221 tcttatatcc aaaggttcct cagctagaga gtcgattgaa aaatctcatg ttccagtaga
    14281 tgcaactata gttggagtag tcgattcgtt tgaggtgttt gatgaataaa agtataggag
    14341 ctatagagtt taaaagcatt gctaaaggta tagaagtatc caatgaaatg ataaagaaat
    14401 cttcagttga tgtattgtac ttaaaaagta tttgcccagg taaatttcta ataatagtag
    14461 gtggagaaac ttcttatata aatgaatgtg tagattatgg aataaaactt ggagaaggat
    14521 atattgtaga taacttcgta ataaatgcaa tttcacaaga aatattagat ggatttaaaa
    14581 ataagtatca aaagctagat agtatagtct ctataggcgt tgttgaaagt agtaaaatat
    14641 gcgctggaat aaaaatgctt gataagactt taaaatctgg cgacttagta ttagtaaaac
    14701 ttcaattatc ttttgctata ggtggcaaat tagtatatat agtagcagga gatttaagta
    14761 gtttagaata tgcattaaaa gaaagtgaaa atgtagtaag agaaaaagaa gtaatatata
    14821 agactgtaat accatcagta gacagtcaaa taataaaaag tctaataaaa taatgggggg
    14881 aattatgagt ataaacgaaa ttataatcta tgtaatggta gtcttcatgg tacttggagc
    14941 tatagataaa tgtataggta acaaatttgg tcttggagaa caatttgagg aaggtataat
    15001 ggcaatgggg tcacttgcag ttgcaatggt tggtgttata tgtttagcac cagttcttgc
    15061 tgatgtatta agaccaattg tagtaccagt atttaatata cttggagctg acccagctat
    15121 gtttgctgga agtttattag ctaatgatat gggtggtgcg ccacttgcac ttgaacttgc
    15181 gaaagaccct aatgctgggt tatttggtgg attaatagta ggggctatga tgggacctac
    15241 aatagtattt ataataccag ttgcgcttgg aataattgaa aaagaagacc aaaaattctt
    15301 agcaacagga atacttgcag gtataataac aatacctatt ggagcattcg ttggtggatt
    15361 agttgcagga ttcgaggtaa tgatggttct tagaaactta gtgccaataa taatatttgc
    15421 agtagttata gcacttggat tattaaaatt cgaaaatgct atgataaaag gattcacata
    15481 ctttggaaaa ggtgtagtaa tagttataac tttaggactt gctgctgcca tagttgaagc
    15541 tttaacagga atagttgtaa tacctggaat gaaaccaatc agtgaaggta tagaaatagt
    15601 tggagatata gcaatagttt tagcaggagc attcccatta gtgtttgtta taacaaaggt
    15661 atttaataaa ccattaatgg ctttaggaaa agttcttgga atgaatgatg tatcagcagc
    15721 aggccttgtt gcaagtttag ctaactgtat accaatgttt ggtatgatga aagatatgga
    15781 caatagagga aaaataataa atgtagcatt ctcagtatca gcagcattcg tatttggaga
    15841 ccacttagga tttacggcag gatttaattc taatatgata acaccaatga tagttgctaa
    15901 attagttggt ggtataacag cagtattgtt agctatggtt atcgcaaata agacactaaa
    15961 gaaggagaat gtataagatg gatatatcaa atatagataa aaatttaatt gaaacattag
    16021 taagacaaat aatagaagaa aaaataagtg gaactaaaga cacagtagat tttgttagaa
    16081 ataaagacat tagtggaata acttctataa agttaccaac tgtaaaagtt agtgaatctg
    16141 atagattaga tacagggaat ccaagtgatg tagtttacac aaaagattta tttacattag
    16201 aggaaagccc aagattaggt tgtggtatga tggaaatgaa agaaacaact tttgattgga
    16261 ctctaaacta tgatgaaata gattatgtaa tagatggaac tttagatata ataatagatg
    16321 gaagaaaggt ttctgcttct tcaggtgaat taatattcat accaaaagga agtaagatac
    16381 aattttctgt acctgattat gctagattta tatatgtaac atatccggca gattgggcat
    16441 ctcaaaatta ataaaaatca agttaagtta ataataatca aataaataat ttaaaataca
    16501 aactcttaaa taagcac
//
LOCUS       DAFKMX010000089        16476 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFKMX010000089 DAFKMX010000000
VERSION     DAFKMX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.089"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(132..1076)
                     /locus_tag="LZB08_002199"
     CDS             complement(132..1076)
                     /locus_tag="LZB08_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBM7315697.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            1394..2824
                     /locus_tag="LZB08_002200"
     CDS             1394..2824
                     /locus_tag="LZB08_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315698.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSNFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            3024..3608
                     /locus_tag="LZB08_002201"
     CDS             3024..3608
                     /locus_tag="LZB08_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBM7315699.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(3702..4481)
                     /locus_tag="LZB08_002202"
     CDS             complement(3702..4481)
                     /locus_tag="LZB08_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBM7315700.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(4539..5795)
                     /locus_tag="LZB08_002203"
     CDS             complement(4539..5795)
                     /locus_tag="LZB08_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBM7315701.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            6053..6952
                     /locus_tag="LZB08_002204"
     CDS             6053..6952
                     /locus_tag="LZB08_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBM7315702.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            6976..8424
                     /gene="ddlR"
                     /locus_tag="LZB08_002205"
     CDS             6976..8424
                     /gene="ddlR"
                     /locus_tag="LZB08_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBM7315703.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ATELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            8483..9289
                     /locus_tag="LZB08_002206"
     CDS             8483..9289
                     /locus_tag="LZB08_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBM7315704.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            9315..11234
                     /locus_tag="LZB08_002207"
     CDS             9315..11234
                     /locus_tag="LZB08_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBM7315705.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(11476..11823)
                     /locus_tag="LZB08_002208"
     CDS             complement(11476..11823)
                     /locus_tag="LZB08_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBM7315706.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(11810..13738)
                     /locus_tag="LZB08_002209"
     CDS             complement(11810..13738)
                     /locus_tag="LZB08_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBM7315707.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKIPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(14149..16338)
                     /locus_tag="LZB08_002210"
     CDS             complement(14149..16338)
                     /locus_tag="LZB08_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBM7315708.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         6091 a         2512 c         2093 g         5780 t
ORIGIN      
        1 attttatttg tactgtccac tttattctat aaaatttata aatagttatt ttttaatatc
       61 aaatataatt tggtataatt tttgttggac tctaaaatgt ataccaataa atccttttta
      121 agtatataat tctaaatact ttcagttttt tcaatctgct ttaacttgtt ggacttataa
      181 aatattctta tttcatcata agaaagatta agtgctacta ctgcagatat taacatcaac
      241 atcactccaa ataacttccc taaggaaaaa ctttctccta atatagtgca accaattatt
      301 actgaaccaa ctaattctac tgatgccaca acacctgcaa ctgacaactc tacaccttta
      361 gatatacctg ctgcataaaa aatatatgca agtgcagctg gaacaatgcc taacattagc
      421 atacaagata aaacatctaa atccttagca taatttaaga tttcccaagg tcttgaagaa
      481 ggaatcataa atatagctcc aaataagaaa ctgtatacta atattgttga acttgaaaat
      541 tcctttaaag catttttact aatagttggc ataagtgcat atgctattgc tgataatacc
      601 ccaagtaaaa gcccaaaagc gtttaaacct tgaaaatcca aacgtccacc agtcactgcc
      661 ataattgcac caataaaaca aagaataagt gaaaataatt tgcttctagt aatattttct
      721 ttataacata attttgaaaa tattgctaaa aacagtggtg atgtatatag taaaactgct
      781 gcaattgata cacccgcaat gtcaattgct ttaaaataac ataaattaaa cattgcctga
      841 catataattc ctattattac tgaatatata attccttttt tgtttatctt taagatttgt
      901 ggtgtcctta tagatgaata tacgcctaaa actataaatc caaaaaacaa tcttgtaaat
      961 gctacttgtt ctgatgtcag tccatttccc attaaaaact ttccaaataa tccaagtgta
     1021 gcccataata accctgctat tgctataaat acataccctt tacttttttc tcccatttta
     1081 aattcccttc tatttttatt tagtcaattt acactcttct ttttaagcaa aaactatgcc
     1141 atcttaaatc aatatatatc gactctaaaa ccctagtctt tccaataata ctaataaatt
     1201 ttcaattttt ttatatttat ttttcgctta ctttaagttg atttagactt attttaattg
     1261 taaaatgagt tatatataac ttatttaaag ttaaaataga ctgaaactta tatataaata
     1321 tcaaatttta agtatattaa tcaattaaac ctattttaat atatcattaa aaatttagga
     1381 ggaaaatata aaaatgaatt tagatttgga attttatcaa aaaatattag aagcttccca
     1441 tgatgaaata tgtgtttctg atgataaagg aataataata tattgtaata aagcatttga
     1501 agaaaattat ggtctaaaaa aagaagatat tttgggaaaa aatgtttctt ttttagaaga
     1561 ctctggatat tcaacaaaaa gccccatacc aattgtatta aaaacaaaga ctaaattttc
     1621 actggaacaa gatactcaaa cagggaaaaa gcttatcata actgctaccc ctatatttga
     1681 tgaaaatgga cacttagaat ttactgttga aaattgtaga gatataactg aacttaataa
     1741 tataaaaaat aaacttgaag acacaaaaaa gcaagtaaaa aaatataaat ctgaagtaga
     1801 aacactatac agaactgcac ttagaattga agatactgta attatggacg gaatagttat
     1861 gagaccaatc ataaacacag taaatcatgt ttctaaaacg gatgtaagtg tacttttatt
     1921 gggtgaatct ggaacaggaa aaagctcact tgctagatat atacaccaca acagcaatag
     1981 atctaatggc ccatttataa caataaattg tgcaactata tctccacaac ttcttgaatc
     2041 tgaacttttt ggatatactt caggtgcttt tacaggtgca agtactaaag gtaaagttgg
     2101 gctcgtagaa ttagctaatg gaggaactct ttttttagat gaaataggag atataccaca
     2161 aaatctacag gcaaaatttt tgcaattaat acaggataga acttttactc cagttgggag
     2221 cttaaaaaat aaaaaggtcg atataagaat tatatctgct acaaatgttg atttagtttc
     2281 aaaagtaaaa gaaaaaaagt ttagagaaga tttatactat agattaaatg ttatagaaat
     2341 aaaattgcct cctcttagag aaagaaggga taatctagtg gaaattataa aatattattt
     2401 taataggtac tcaagtaact tcaatctaaa taaaactatt tctaaagaag ctatggatgc
     2461 tattgcaaat tatagattcc caggcaatat aagagaatta caaaatataa ttcaaaaaat
     2521 cttgcttact tgtacagata atcatataac aatagatgac ttaccaaaca ttcttacaaa
     2581 aaatatacat attaccaata atggaaataa aacacatata tcccaaatta ataaggttat
     2641 tatatctgac tcaaaatcta ctaattataa aaataaaaat tttgacactt taattaaaga
     2701 gtatgaaaaa aatataattt tggatgctta tgaaaaattt ggtagttctt ataaagtagc
     2761 caaacacctt gaaatctcac agtctaaagc caataggtta attagaaagt atactaatac
     2821 ttaattttaa atttgataaa taaaaactta acactattta catattgtaa atttggccat
     2881 ttactgttat tatgaatatg gttgaaatta tttaaataaa tactgttgac ttagtttcat
     2941 atatttacca ttaaactttt atcataaata tcatatcata ataatatatt taataagtct
     3001 aataaactat acgaggtgta aatatgttaa tgaataccca ttttattatg gcaaaatcaa
     3061 tacttgataa tatagatgaa aataaaacat tctttataag cgaaaaaaat tttatatatg
     3121 gcaatataaa gcctgatgct ttttctaaat ataaattaaa aaaacattat ttagatgagt
     3181 catttaatat gattgtttct aaaataaact atctatgcag tcttaatata gatagtcttt
     3241 ctaaatactt ttcagtaagt agacttagtc aggaactagg agtcatatgt cattttttat
     3301 gtgatttttt ctgtgtagct cacagtgaac gttgggaatt taagcatagc ttaaataaac
     3361 atgttactta tgaaagagaa cttggagcat ttgcaaaaga tgttgattta tctaaaatta
     3421 aatgtggtac tataaatagt agttttgaga agttttttac taaactttat agtgattaca
     3481 aaaagaaaac tgattatatg aatgatttaa aattttcagc ttatacttgt aatagtgtca
     3541 taaattatat acttgactct attttatcta atacaataaa atcatatcat atagcaaatt
     3601 gcggttaact taagaatttt taaacttata attgaatata attaaaatga ctgctctata
     3661 gaatataatt ttatacttct ctaggacagt cattttttat actaactatt tatctccatt
     3721 ataacatcta tggcactttt tacactatct actacataat ttgcactttg ctttacatct
     3781 tcccttgcct tagacattgc aaaactatat ttaaatcctt ctaacatgca aatatcattt
     3841 ggagaatccc caatagtagc aacttcatct ggacttacat ttaacgtttt tattacatgc
     3901 tctatacttc ctctttttga cacactttta ggaactatat ttaaagtata tgttccagaa
     3961 aaatatattt ctaagacttc actaaactca ctttttagat aaagttctat tctggataac
     4021 tcttccaaat tttcagaaca tacgcctata tttcctataa gattatcaaa actacttctt
     4081 ttttcaaaat ctgggtcaac tataaagtca ctagaataat gtctaaaagc agtggtatca
     4141 ctacccacaa ttacatcacc cttatattta aaataaagtt gtgcatcact tgactcaaat
     4201 ctatcaatga ctttttttac taaattatca tctatcgttc ttttaaaaat tacattataa
     4261 tctttatcat atataaatga accattttca catacatagt atccattcat atctaaacta
     4321 tctttgataa tattatgtgc ttgtttaaaa attctacctg tagcaacatt aaactgtact
     4381 cccttttttt ctatttcttc aattgctact atatcttctt ttgttatggt tccattttca
     4441 tatagagttc catctaaatc acaaaatata tgttttatca taaattaaaa gcctccaaaa
     4501 acaattttat attttactaa tactttaatc aaaataactt aaagcttttc taattgtatt
     4561 ctcaattcat taagcaaatc tctagtagct cttaatctta tctttcttct atccccatta
     4621 aatatatttt tctttactat tgtttttcca tttatatata ttgcagtata aaccagtccc
     4681 acaggcttct ctttggttcc tccaccaggt ccagctactc cagttgtaga tatacctata
     4741 ttagctttaa gtcctttagc aacgccctca gccatttcta tagctgtttg ctcacttact
     4801 gctccaaact cttccaaagt ttctcttttt actcctaatc ttttcatttt agaatcatta
     4861 gaatatgtta cacaaccttc aagaaataca gaggatatac ctggatagtt tataagagat
     4921 gaagaaacca aacctccagt acaagattca gccactgcta ttgttagatt tttttctaca
     4981 agtagcttag aaacagcatc ttctaaagca gtctcccctt cagatatgga aatatctcct
     5041 tcagtatata catatttacc aactctactt tttatttttt cttctactgg ttttattaat
     5101 ttaaaagctt ctctttcatt ttcagccttt gcagttattc ttattgtaac ttctaattct
     5161 tttgcatata aagcaactgt tggattagtc tgctcattaa ttatatcgag aatttctaat
     5221 tctagatttg actcacctat tccatataat cttaaagtct tagatactag catttcattt
     5281 gtgaattgtt gtaagtaagg tttgacactc tcattaaaca tagctttcat ttctcttgga
     5341 ggtccaggca atactattat aaatttacca tcttttttta aaattgcccc tggagctgta
     5401 ccattattat tttttagtac tatagcatct tttggaaagt acacttgttt catattattt
     5461 tcggccattt ttttccctgt tttaacaaag aaagattcca aaacctctag tgaaggttta
     5521 tgtagttcta atttctgacc aaagtattct gcagcagttt cttttgtcat atcatcacca
     5581 gttggcccaa gaccacctgt agtaattaca aaatcacttc tctttaagct ttcatcaaaa
     5641 cattctaaaa gtctttgttt gttatctcca acagtactct gatggtatac ttctattccc
     5701 aatgatgcaa gttctttagc taaaaattga gaatttgtat ttactatatc tcccaataat
     5761 atttctgttc ctacacttat tatttcagct ttcaaaattt atcactcctt gatttatagc
     5821 cattaagact agtcttaagt tcataacaat aattttagca aaaataataa cataaataaa
     5881 gttaaaaaat catttaaaca tatctttaaa ctagattatt attctaaaac cacacataat
     5941 ataaacttcg tatatagtgg tactttcatc ctcaactgtt aattcatata attaagtatg
     6001 taaataaaaa gtttacttta aaataataaa aattaaaata ggagtgtata atatgaaaat
     6061 agcagttata atgggtggaa tatcatcaga gagagaagtt tctttaaatt caggaaaaga
     6121 gatatataat aaccttgata aaaataaata tgaagttgta aaggtaataa tagatgataa
     6181 aaaagatata tttacaaaga taccagaaga tatagatttt gcaatacttg ctttacatgg
     6241 aaaatttggt gaagatggat gtatacaatc aatacttgag actatggata taccatactc
     6301 tggatgtgga cctctttgca gtgggatgtg tatggataaa aatataacta aaaaaatgct
     6361 tagagattca aacctaccta ctgctccatg ggttttagta aaatcagttg atgaaataga
     6421 ctatgatgaa atagataata taggttatcc agtatttata aaaccaaact caggaggttc
     6481 aagtgtagcc acattcttta ttcattctaa ggatgaagtt gaagaagcag ttagaaaagg
     6541 tcttgaagtt gatgaatttg taatgattga aaaatatata ccaggtggag aatatacttc
     6601 attcatatta aatggagaag tgttccctac aatatctata aaatctgatt caggattctt
     6661 tgattatgaa gctaaatact cagttgaaaa aggtgcaaag gaagaagttg tttacttaga
     6721 tgaagaactt caaaaaagag taaacgaaat ttctgagact tgttggaaaa tttttaattg
     6781 caaagcatat gtaagagttg atatgattat aagtgaaggt attccatatg ttcttgaact
     6841 aaatactctt cctggaatga ctcaaactag tttaattcct agaagtgctg ctgctagagg
     6901 tattaagtac agtgaactat tagataaact tatagaatac tctttaaatt agatgaaata
     6961 atttgaggtg gtaatttgat attttcaaat ctaaaactaa atgataatga accaatttat
     7021 atacaactta aaaattatat aagtgatatg atatcaaaag gactaattcc agacaatagt
     7081 aagctccctt ctacaaggga gcttagtcag cttttacaag ttagccgaaa ttctgtagtt
     7141 ttaacttatg aagaattaaa atcagaagga ttaatctact ctatttcagg aaaaggtaca
     7201 tttgttaaat caaagaataa gtcttcaaat actacatggt cattaaactg ggattgttta
     7261 gagaatacat actctaaaaa agccactgaa ttggacatta taaaaagtga aattccttgg
     7321 agctctgacc tgatttcctt taaaagtatt tctccagatg gagatttatt cgacatggaa
     7381 gaattaaaaa aatccttttt aaatagaatt tcactagaag gacataaatt actcaactat
     7441 ggatatgcac aaggttataa gccacttatt gactatttac tagaatatat gacaaacaag
     7501 ggtgctgata taagtaataa agatatacta ataacaaatg gattcacaga aggctttgat
     7561 attattatgt catcttttac ccaagaaaaa gactacataa tatgtgaaaa tcctactcat
     7621 aatactgcta taaaaattat gaaatctcat aacctcaata tcttaggtgt agatataaat
     7681 gaagatggtt tggacttcaa tatgctagaa gacaatttag ttaagtataa aaataaaata
     7741 aagtttgcat acattacacc ctcttatcac aatcctacag gaattgtaat gactcctgaa
     7801 aataggtaca aattctataa tcttatgaaa aaatacaata tagcaattat agaagatgga
     7861 tttaatgaag aattactcta caatagttct catatatttc ctatctgttc cctagataat
     7921 atgaataatg gggtcgtata tattggtagt ttttcaaaaa ttttatttcc tggaatgaga
     7981 attggatgga tatttgcaga taaaaaagta atcaataagc tagaaagtgt aaaaaggtgc
     8041 agaaacatac atgtatcctt tttagaccaa ggaattttat ttgattattt aagtaatgga
     8101 ggttttgaaa aatacattaa aaaaatacgt aaattctatg gagacaaatt taactttgca
     8161 tataagtgta taaaaaaata tataaaaaca gaatatatct tgggtgatgg tggtttgcat
     8221 atatttataa aactaaaaaa tatagatact agagttttat tagaaaaatg ttatgaaaaa
     8281 aatgttatat ttatgcctgg agatttattt tttacagata actctggttt tgatacttta
     8341 agactcggat tttctaggtt gtcttttgaa gacattgaaa ttggaataaa aataattgga
     8401 gaaactataa atgaaatgtt ctaggttaac taaatattgt tagcctagtt tatttttatg
     8461 aggtataata tatatagttt atatgaatct aatactgggg gataatatga gtaaaaagtc
     8521 aaaacgaaga aaaattaata aattaaaatt aacgattgta ataactgtat tatttcttgc
     8581 tttattatct atatatgaga tactgttctc taatcccaac aaaaaaggta atataaagag
     8641 tcaagaaact atgcaaaata aaaataatac aacagatgat tctaaaaata atcaaagctc
     8701 taccaaaaag caggaagata ataatatcaa acctactttt tcatattctt caataccaga
     8761 taatataaaa aataaaatga ttggtaaatc tatgcctact gatgaaccta taagctttga
     8821 tagtctatct tatttaaaac ttacatatta tggatttgat gaaaaaactc atcaaggaga
     8881 aatgatagta aacagtgaac tagctccaga agtagtggat atttttaagg aattatacga
     8941 aaaaaaatat ccaattgaaa aaattaagtt aatagatgac tatgatgctg tagatgaaaa
     9001 atctatgtct gataataata cctcatcatt ttgttataga actatagctg gtacaaatgt
     9061 agtttcaaat catggtaaag gacgtgctat agatataaac ccattacaga atcctcaagt
     9121 ttctggaaat gatgttacac ctaaagtaag cacagtttat gctgataggt ctagcacaaa
     9181 atttggaatg ataaaaaaag gtgatgattg ttataatgct tttgtaagca gaggttggag
     9241 ttggggtggc tattggaaaa acccagatta tcaacatttt gaaaaataat agattagaaa
     9301 taaaggtgaa ttatatgaat ttgatgacat tggaaaatat aagtaaaagc tattctgaaa
     9361 aaaaattact agaaaatata tctcttggaa taaatgaagg ggaaaaaata ggacttattg
     9421 gtgtaaatgg tactggtaaa tctaccttac tcaaaattat tgcaggagtt gaagaaagtg
     9481 agactggtaa tataacaaaa actaatggta ttaggataga atatctacct caaaatccaa
     9541 cttacaatga aaattcaact gtacttgaac aagtatttaa tggaacttct gaagaattaa
     9601 agctacttgg tgaatatcaa gaaatcctag aaaaacttaa ttcaaacttt acagaggaat
     9661 taaataaaaa attattatca gtacacgaaa aaatagatgc cttaaatctt tgggatttag
     9721 aaagtgaagc taagtcagta cttacaaagc ttggcataaa tgactttaat caaaaaatag
     9781 cagaactttc tggtgggcaa agaaagagag tctctctggc atcagccctt attacacctt
     9841 gtgagttttt gatactagat gagcctacaa accatctaga caatgatact atagattggc
     9901 ttgaggaata tcttaattcc agaaaaggtt cattgcttat gataactcat gatagatact
     9961 ttttagaccg tgttacaaat agaattattg aactggacaa gggtagatta ttcagttatg
    10021 atgggaatta ttcaatattt ttggaaaaga aaatggaaag actttcgcta gaagcaagta
    10081 ttgaagataa aagacaaaat ttaataagaa cagaacttgc gtgggtaaga cgaggagctc
    10141 aggctcgtag cactaaacaa aaagctcgtt tacaaagatt tgatgaactt ataaatagag
    10201 attcttttaa acctgatgaa aatcttgata tatctgttgc atcaagtaga cttggaaata
    10261 aaataataga aatacacaat atatctaaat catttgaaga aaatactgtt atagataatt
    10321 tagaatacac tttaactcgt acagatagaa ttggtataat aggcaaaaat ggtatgggta
    10381 aatctaccct tataaatata cttaatggag aaatagagcc tgatagtgga tatatttcaa
    10441 ttggagaaac ggttaaaata ggttgttttt ctcaagacga ctctcatatg aatattgata
    10501 tgagggctat agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta
    10561 ggattacagc ttctcaaatg tgtgaaagat tcctttttaa tggtactatg caatatactt
    10621 tgataggtaa attatctggt ggtgaaagaa gaagactaca tcttcttaaa atccttatga
    10681 cagctcctaa tgtgcttctt ttagatgaac ctacaaatga tttagatatt gaaacgctta
    10741 aaatattaga agaataccta gacgaattta aaggtgtagt agttacagta tcacatgata
    10801 gatatttctt agatagaatt tgtaataaaa tatttgcata tgaaggtaat ggaaaaattc
    10861 atatctatac aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat
    10921 ttgaagaatc taaaaaggaa tcagttaaaa aagatgactc tactacccta aaaaaagaaa
    10981 aacctaaaaa tgataaaaag cttaagttct catataatga acaaagagag tttgaaaata
    11041 ttgactctga tatagaaaaa ctcgaatcta aaattgcaac acttgatgaa agtactgcaa
    11101 aattttcaac tgattttact aaacttcaag aaattttaga tgagaaatct aaactagaaa
    11161 aagaacttga atacaaatat gaaagatggg aatatttaaa taatctagct gatgaaatag
    11221 ctaataataa gtgatattaa aataatctta agtttaataa gttttacttt aaatctgtac
    11281 tttataaaca ttttaaagga agtgtctcaa aatgaacttt ttagttttat gagacactct
    11341 tttttatgag aaatcaaata tgttttcatc atttcaacaa tacaaaagag gcttatctct
    11401 attttgagac agcctctttc ataattacct tctaatttat atgctctaat atatctataa
    11461 taattcttat tatatctata tatccagtct ttcaatatag tgacaattaa cttcaatatg
    11521 atttaaaaat actaacaacc tttcataaag tggatgagat tcttctccat atttagcttc
    11581 taggttcttg cctatttctc taacagtcct ttgaccatca atttgtaaaa aaacaaaact
    11641 ggaatattca tctagttcta tttttttgta tttaggtatt ctagctttta actttctaaa
    11701 aaacttttga attttatggt cctgtttttc aagtatagta acaacactat tttcagctac
    11761 ttcatattca agtccatcag taattttaaa tacaatatct aaaacatcat tactcttgct
    11821 cattattttt tactttagat aaaacaatag gtaatgctgt aagcacaact aaaactacca
    11881 ataaagcaat tgccattgaa tttgttgcag caaatccact tggtatcttt ggagttacaa
    11941 ctccagttac ttgtaatatt atacctatta aaccaatgat agaaccacca gcaacaagac
    12001 cagaagataa acttatacca ttagaaactt taacttcttt ttctttttca gttttagata
    12061 ctaattcaac aaataaacga attaatgctc caactaatat tatagaagtt gttgcaattg
    12121 gtaaatagaa tcctatagct atagtcatta ttggtaaatt taatgcatat agaactattg
    12181 ccataaatac tccaacaata atcataaccc aaggtaaact acctgacata ataccagaag
    12241 ttaatgttga cattaagttt gcttgtggta atgcaaattg tacattatca ccagttgctc
    12301 taagttggtc agaaagtaat aaaattactc ctacaacaac tataacacca actatacttg
    12361 caattgtaaa gtaacgttgc atttcgtttt tgctaccacc aataatataa gttacttttt
    12421 gagattgagt ataacctcca gctatagcaa tagcaacaac tataaatgaa ccaaataaaa
    12481 gtaatgattt attagcttct aaatcagtcc atcccattat aacaaacact aatgttacaa
    12541 taactagaga agctatagtc ataccagaaa ctggaagatt tgatgttccg attgttcctg
    12601 ttaaacgacc agctactatg acaaataata aagataataa aagagatatg atagctccaa
    12661 taattgccat aacaatgtta ccagaaatta agaatgctgc taaaaatcca ataactactc
    12721 cacctaataa aagtatcatt tgtatagatg aaccttcttc tccctctcca gcacttgatt
    12781 tagctttaag tgtttctttt atagaagcga ttatagttgg tataagtttt attgcaccaa
    12841 taatacctcc acaaagcatc ataccagcac ctatatattt tacataacta ctagatattg
    12901 ctccaacatc catttggtta agaggcattg caggattatt ccataccata gctccatctt
    12961 tagccatatc tgtaaagtat cctattaaag gtgctatacc aaagtttgat aaaatacttc
    13021 ctgcaaacat tgttaaagaa acttcaagcc ctactataaa tccaattcct aaaagtagag
    13081 gatttacttc aatatcaaat ttccatttgt agaatttgct tcctacaaga ctcattacat
    13141 tgtttgctac atttaagaat gaaccagtta atacagtaat aaatccactt attacaaatc
    13201 caattcccat gtacttaatt gaatctccac cagcctctga agcaacaagt gtttcagata
    13261 tagccattga ttctgggtac ataagtttac catgttcttc aactattaga tagttgtgaa
    13321 ctatagcagc aattcctata ccaaataaaa ctccaccaac tccaacagct aagccttcaa
    13381 agaatgtaat ttggcttcca attaatataa ctgctggtaa tacgaaaata aatccacttg
    13441 caacagattc tccaccactt gacatacctt ggattaagtt tttaccaaga ataccctttg
    13501 aacgagcaaa tgctccaaca aaagcagaac ctattatagc tcctggaata cctgcggcaa
    13561 ctgtaagacc ggctttcatt ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa
    13621 taataccgat aatcagtaca gctacatttc caccggttct ggacttatct gtgatatatg
    13681 gaacgtaatc ttttccacta acctcaccat aagcaccctt aggtaatttt ttgttcataa
    13741 aattttcacc ccttttcaat ttgtatatat tataacatat atacatataa aacttaactt
    13801 tttattattt tacaaatagc gagtcaaaac tactaaaatt taaccctata ttattaattt
    13861 ctacttttag atatttttat gctaattttc tttttatttt tcaattctac taaaaactat
    13921 taagatataa aataaaaaaa ttttttaatg taaaattaat tttgtaaaaa aaatgcttta
    13981 aaacacggaa aagtataaaa aatacaataa tcattcttat aaataaattt tcaccatagt
    14041 aaatatatac agaaaattct tgtttttatg caaattgatt cttttataaa aataagtgtc
    14101 ataaagaaat gtctgaaatc actttatgac acctatcata ataaaaaatt attctggtaa
    14161 tgctctttta tttacttttc cgattggtgt aactggtatc ttatctatcc tcattattat
    14221 tgttggaatc atatatggag gtagatgctc atttaaatat tctctaatag ttctttcatt
    14281 tatcttacca ctatcttcct catcaattac ataatatgca catatataca tatctccatc
    14341 atcatttgtt ctcgatatac aaacaacact tttaatttct gtaatattat ttattatatt
    14401 ttcaatctca tatagctcta tcctaaactc atcaattttt acttgattgt ccatccttcc
    14461 aatataatct aaatttccat catctaacca tcttactaaa tcaccagttc tatacattaa
    14521 cttattatgt tcacaagttt tgtaaggatt ctctacaaat acttgctctg ttagcccttt
    14581 tttatttaaa tatcctcttg atatttgaat gcctgataga catagctctc ctggtgctcc
    14641 aattggacat agtttgttct ttttatctaa tacatatgca taagtattaa atataggttt
    14701 tcctattggt atttttccat attctctgtc cacatgaaaa gaagtcgata caacagtaaa
    14761 ttcggttggt ccatactcat tatatagttc ataattacgc tttctatatc cttttaatct
    14821 ttctcctccg gtctgtacaa ctctaagtga attattattg caattttgta taaataacct
    14881 tgcaaatgct gttggcaatg ctagtatagt tatgtcattt tcctcacagt attcatttac
    14941 tgtatattca tcaaatttag cttcttcagg cataatatct attgatgcac ctacaagcaa
    15001 ataagggaat agttgtttca ttacagcatc aaaactgaaa cttaagtata taccacatat
    15061 atcattttct gtagcgctta tagattttac gctataataa cacatattta ctaaactctt
    15121 atgttctata gctatggtct ttggcctacc agttgaccct gaagtagata tcgtatatgc
    15181 acaatttgat gatgttattt tttcagttaa ttcatatcca agcttaaaca tttcttcatc
    15241 atttatatcg agtactatgg tattctcatt tacaacctta cttttgagtt catctgtagt
    15301 tatcactact tttgcattac tatcttcaag aaggtaattc aatctttctt ctggattact
    15361 tgtgtctatt ggaaaaaatg ctccccctga tttcataatc cctattgctg taataattat
    15421 gttaatatct cttggtaaca ttacagctac tatgtcttca ctttccactc ctatatgatt
    15481 taaataacct gcaagtttat cagttacttc atccaattct ttatatgtta actttctatt
    15541 ttcatcccta gcagctatat tactaggtgt taccttaact tgctccctaa ataaatcaac
    15601 ataactttta tcatatggta tttcatattg attattatta aagtcttcca ataatacttt
    15661 ttcttcttcc ttacatacaa attctaaatc ttctattaaa atatcattat cttctattat
    15721 ttgctctatt atataatcaa attgatttaa aattcttcta atagtttctc ttttatatat
    15781 agatggttta tactttattt ttattttaag tgccctctta gtatatacta ttacagataa
    15841 atcatatcct tttttttcta tgtcataatc atatgataaa tcaaaacttt tagctataaa
    15901 tattgtgttt ataagcttag tatttagatg atttttattc tctatatctg ataacaaata
    15961 gtatccatac tctaaagacc tgctccactg cctttttagc ttccttacaa catctataaa
    16021 tttttcactt tgtctaactc ttactcttat tggaattata ttcattgttt taccaaaaac
    16081 tacatcatct tcatatatat acttttgaag tataaggcca tatatagttt ctatgataga
    16141 gtctatgcta atattgttca ctttagaata ttgaattaac ctttcatgtt tctcaatatt
    16201 catcttaaaa acctcatcta ctacttcatc agaacaatct ttataaaaaa aatcatttgg
    16261 aatttttgct atattcgtat aatcatctaa caaatccatc caataattca tagctcgttc
    16321 taaattaact tcatacatat caaaattccc ccttactact aagtctatag aaaaaattct
    16381 tttggaattt tattttcaca atatataaat atattaataa tacatatgca agctctaaaa
    16441 acatgagctt gcataaaatt acacttatta tgacta
//
LOCUS       DAFKMX010000090        16453 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFKMX010000090 DAFKMX010000000
VERSION     DAFKMX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.090"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            217..354
                     /gene="rpmH"
                     /locus_tag="LZB08_002211"
     CDS             217..354
                     /gene="rpmH"
                     /locus_tag="LZB08_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBM7315709.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            432..776
                     /gene="rnpA"
                     /locus_tag="LZB08_002212"
     CDS             432..776
                     /gene="rnpA"
                     /locus_tag="LZB08_002212"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBM7315710.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            808..1059
                     /gene="yidD"
                     /locus_tag="LZB08_002213"
     CDS             808..1059
                     /gene="yidD"
                     /locus_tag="LZB08_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBM7315711.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            1091..1798
                     /locus_tag="LZB08_002214"
     CDS             1091..1798
                     /locus_tag="LZB08_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBM7315712.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1767..2396
                     /locus_tag="LZB08_002215"
     CDS             1767..2396
                     /locus_tag="LZB08_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBM7315713.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2508..3887
                     /gene="mnmE"
                     /locus_tag="LZB08_002216"
     CDS             2508..3887
                     /gene="mnmE"
                     /locus_tag="LZB08_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBM7315714.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            3982..5877
                     /gene="mnmG"
                     /locus_tag="LZB08_002217"
     CDS             3982..5877
                     /gene="mnmG"
                     /locus_tag="LZB08_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBM7315715.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            5870..6589
                     /gene="rsmG"
                     /locus_tag="LZB08_002218"
     CDS             5870..6589
                     /gene="rsmG"
                     /locus_tag="LZB08_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBM7315716.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            6752..7537
                     /gene="noc"
                     /locus_tag="LZB08_002219"
     CDS             6752..7537
                     /gene="noc"
                     /locus_tag="LZB08_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBM7315717.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7672..8445
                     /locus_tag="LZB08_002220"
     CDS             7672..8445
                     /locus_tag="LZB08_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBM7315718.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8447..9310
                     /locus_tag="LZB08_002221"
     CDS             8447..9310
                     /locus_tag="LZB08_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBM7315719.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9343..10485
                     /locus_tag="LZB08_002222"
     CDS             9343..10485
                     /locus_tag="LZB08_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBM7315720.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10910..11809)
                     /locus_tag="LZB08_002223"
     CDS             complement(10910..11809)
                     /locus_tag="LZB08_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7315721.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLERDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEINRISRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQKHPNIEIHILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEVEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGIAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSQYDSFA
                     VQKMKEHIVSYTKEKGFFNFKSF"
     gene            12079..13275
                     /locus_tag="LZB08_002224"
     CDS             12079..13275
                     /locus_tag="LZB08_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7315722.1"
                     /translation="MVKFAKRMENMKNSANIVRNLFNSMTDPEIISFGGGAPAIEALP
                     VDIVREISNEVLAKNKRGIEALQYSHPYGLKDLREAVAQKLLEPKGVHASLEDIMIVN
                     GGLETMNLICQVFIDPGDVILVESPTFVHCVEIFEMFQAKCIAVDMDEHGIIPEDIER
                     KIQKHHPKMVYVIPTFHNPTGRTLTTERRKILAGIGSKYDVLILEDDPYRDLRYSGEE
                     LPPIKAFDQTGHTILANSFSKIFSPGARLGYVLAEHAIIERLLDAKTATNSHTSAISQ
                     VLCAEFFNRGYFDEHLKNIRDIYRERRDVMIESIDKYLPKGTKRVFPDGGLFTWVELP
                     ENINTTELLKEAVEHKVAFVAGEGFFTEGGGKGRNCMRISFGNVPPEKIRIGMERLGQ
                     LIASKM"
     gene            13343..14089
                     /locus_tag="LZB08_002225"
     CDS             13343..14089
                     /locus_tag="LZB08_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBM7315723.1"
                     /translation="MTNKVPNLWNQLENLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSN
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNVSGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            14122..14625
                     /locus_tag="LZB08_002226"
     CDS             14122..14625
                     /locus_tag="LZB08_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBM7315724.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            14873..15706
                     /gene="panB"
                     /locus_tag="LZB08_002227"
     CDS             14873..15706
                     /gene="panB"
                     /locus_tag="LZB08_002227"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBM7315725.1"
                     /translation="MKNTIQTFKNAKSEGKKLSMLTAYDYSMAKIMDECNINGLLIGD
                     SLGMVVKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LVKEGGAHAVKLEGGANVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGITSEA
                     AKQLIEDAKLIEKAGAFSIVLEGVPAKISEIITNSISIPTIGIGAGINCDGQILIYQD
                     MLGIFEDFVPKFVKQYANVGDIMKDSIKNYILEVETGAFPQEKHSFSIKESELEKLYE
                     D"
BASE COUNT         6525 a         1849 c         2971 g         5108 t
ORIGIN      
        1 aaaataattt agaataaaat taaatagatt tttactagta ttttcaataa aaaaaataca
       61 taatatatat atggtagatt ttgtgatata agcgtcccaa aaactcgttg acaacagtag
      121 taaaagtatt tataattata tgtgttatgt ttactttgag ggtaaagtta ttatttttat
      181 aaacggaaaa taaaatctga aaggcggtgc aaaataatga gtaaaagaac ttatcaacca
      241 aaaaagagac aaagaagtaa agaacatggt ttcagaaaaa gaatgaagac gtctaacgga
      301 agaaacgtgt taaagagaag aagagctaaa ggaagaaata gattaactca ttaattacaa
      361 aagggccgct gcaaaggtgg cctttttgtg taaataatta caattcatag ctgtgtttat
      421 ggaggttaac tatggacttt aataggacta aagggttgaa aaaagactct gattttagaa
      481 aagtatataa acacggcaaa tcttttgcaa acaaatattt agtaatatat atacttaaaa
      541 ataaatcaga ttatagtaga gtaggtattt ctgtttcaaa aaaagtagga aaagctatta
      601 ctaggaatag agtaagaaga ttaataaaag aagcctacag attaaatatt gatgaaaaaa
      661 ttaagcctgg atatgatata gtatttatag caagggtatc tagtaaggat gctactttta
      721 aagacataga caagtctata aaaaatttag ttaaaagaac ggatatttct atttaattgt
      781 atgctataaa tatggaggtt aatgactttg aataaaataa tttatatatt aaaggaaata
      841 agtaagtatt tatcgaattt atgtatttat ttagtgagat tttatcaaaa gtatatatct
      901 cccttaaagg gacctacctg tagattttat ccaacttgct cacaatattc aatagaggcg
      961 tttaagaaat atggatttgt aaaaggtatg tatttaacga taagaagaat tttaaaatgt
     1021 catccttttc atccaggagg ttacgaccct ctaaaataaa agagagtaac tattaggagg
     1081 tataaaaaaa ttgaatataa taggaaattt cttaggattg attcttaagg taatatttga
     1141 atttgttaat cattatggta tttctataat actttttacg atacttgtaa agattatttt
     1201 attaccatta acaattaagc agactaagtc aacaaaggca atgcaagata tacaacctcg
     1261 tataaaagag atacaagaaa aatataaaaa taagccagaa aaacaaaatg aagaaattgt
     1321 taagttatat ggagaagcta aaattaatcc actatctggt tgtttacctt tattaataca
     1381 gtttcctata ttaataggat tatttagcgt tttgagagaa ccagttgctc atggcgtttt
     1441 tgcaaacaaa gctgcatttt tagcagctga caatgggttt ttatggataa aaagcttaac
     1501 atcaccagat tatgtattag cagtattttc aggagcaagt gcatatgtca tgcaaaaagt
     1561 aatgacacca aaagatcagt tacaaggctc tatgaaagta atgacatatg taatggcagg
     1621 tatgtctttc tattggggat ttatattccc agcaggactt acattgtact ggacagtaag
     1681 taacttattc tcaatagctc aatattactt aataatgaat cctttaaaag ctaaactggc
     1741 tgctaactct aaggaggaaa aagttaatga gaaaaagcct aaaattaata aaaagtaaga
     1801 gtaaagagga agctataagt aaagctatag cagagttgaa tttaaaagca gaggatatag
     1861 aagtagaaat acttgaaaat ccaagtaaag ggtttttagg ttttataggt gctaaagatg
     1921 gtacttatga aatttttgtt atcgagaggg aagtagatgt agctaaaaat tttatagagg
     1981 taatgttgaa aaatgcaaat gttgatgcca aagtcaatgt atctcaaaaa gataatttaa
     2041 tcatggttga tattgaaggc aaagaagctg caagtttaat tggtagaaga ggggaaactt
     2101 tagattctat tcaatttttg actggattag ctcttaataa aataaataaa gattctcata
     2161 caagagtact tgtagatata gaaaattata gatcaaaacg agaagaatct ttaataagat
     2221 atgctaataa agttgctaga gaagtagcga aaacaagaaa aactaaaaaa ttagattata
     2281 tgaatccata tgagagaaga ataatacatt cagctcttca aaatgacaaa tatgtaatta
     2341 catatagtga aggaaccgat ccatatagaa gattagtaat tgagtgtaaa agataaagtc
     2401 aacctcctaa acattagggg gttttatttt ttaatattat taaatcatta atgattggtt
     2461 aaatgtaaat ctatatgtaa aaaattatga attaagaggt gataaatttg tttatagatg
     2521 atacaattgc agcaatagct acagcacctg gagaaggtgg tataggtata cttagaataa
     2581 gtggagaaaa agctttgaag gttgcagaag aaatctttaa atctatgtct ggaaagagta
     2641 ttgaagaata caataagaga acattaatat atggaaatat agtggataat gaaaatataa
     2701 ttgatgaagt tttattggca tatatgaaag gaccaaattc ctatacagga gaagatgtta
     2761 tagaaattaa ttgccatggt ggatttattt cggtaaagaa gatattagaa ttaatactat
     2821 caaaagatgt tagattagca gaggctggtg agtttactaa aagagcattc ttaaatggaa
     2881 gaatagattt atctcaagca gaggctgtaa ttgatgttat aaaagccaaa actgatatag
     2941 ctcatgaagt tgctcaaaat caattagaag gttctttatc aaagaagata agagaattga
     3001 gagataaagt cactgagata ttagctcatg tagaagttgc tatagattat cctgaggaag
     3061 atattgagca tataacatat caaactctta aagaaaaaac tgatgaactt aaaaaagaca
     3121 ttaaaaaatt atatgataca gctgaaagtg gaaaaatact tagagaaggc ttaaagacgg
     3181 taattgttgg gaaaccaaat gtaggtaaat cttcactact taattctatt ttaggagaga
     3241 atagagcaat agttactgat ataccaggaa ctactagaga tgttatagaa gagtttgtaa
     3301 acataaaagg tataccttta aaaattgttg acacagcagg tataagagat actgatgata
     3361 ttgttgaaaa aataggtgtt gaaaagtcta aagaatcatt tactagtgca gacttgatag
     3421 taatggtttt agatgcatct agaaagttgt cagaagaaga tatagagata ttagaaaaac
     3481 ttaaggataa acaaacaata gtgctgttaa ataagaatga tttgaaacaa gagattgaag
     3541 aagaaaaaat acttaaatat gtagaaaata atagtataat aaaaatatca gctttacaac
     3601 aagaaggaat agaagaattg caagataaaa tagagtctat ggtatataaa ggtagtatca
     3661 aaaataattc tagtttagta gtaacaaatt caagacataa agatgcactt agtaaagcat
     3721 ataaatctgc aacagatgct ttaattgctt tggaacaaag tatgccattt gactttgtag
     3781 aagtcgattt aaaaaatata tgggactatc ttggatatat taatggtgat acagtaacag
     3841 aggatttact agataatata ttccataatt tctgtattgg taagtaatgt acaaaagtaa
     3901 acttgttttt attatagaga tttttaaaaa atttgaagtg tttctcatga aacattaatt
     3961 ttgaaaagaa aggaattata aatgataaag tttgaagcag gaaaatatga tgttatagta
     4021 gtcggagcag gacatgctgg atgtgaagct gctttggcta ctgcaagaat gggatataaa
     4081 actttgataa taacaatgtc tttggattcc atagcattaa tgccttgtaa tccatcaatt
     4141 ggagggactg gtaaaggtca attagttaaa gaaatagatg ccttaggcgg tcagatggga
     4201 ttaaatatag acaagacata tatacaaagt agaatgttaa atactgccaa aggtccagcg
     4261 gtacactctt taagagcaca agcagataaa tttaaatatc atgaagaaat gaaaaagaca
     4321 ttagaagatg aaccaaatct tgatatagct atggatgaag ttgtagaaat acttcatgaa
     4381 ggaaatgttg taataggagt tggaactaaa ctaggatgtt catttaaatc taaagctgta
     4441 atattggcca ctggagttta tctgaactca aagatatata tgggtgaagt tgcattttat
     4501 gaaggtccta atgcattagg gtatgcaaaa tatcttacag atagcttagt tgagcttggc
     4561 cttagaatga gaagatttaa gacgggaact ccagcaagag ttcatagaga tagtatagac
     4621 ttctctgtaa tgtctttaca agagggtgat gaaaaagtaa ctccattctc atttatgaat
     4681 gaaaatatag aaaaaaaaca agaaccttgt tatttaacaa gaactacaga agaaactcaa
     4741 aaagttatac tggataattt aaaaaggtct gctatgtata gtggagtaat agaaagtact
     4801 ggtccaagat actgtccttc tatagaagat aaagtagtaa gatttagtga taaaacatca
     4861 catcaattat ttatagaacc agaagggtta aatactaaag aaatgtatat acaaggaatt
     4921 tcaactagtt taccttttga agttcaatta gatatgtaca aaactataaa aggacttgaa
     4981 aattgtaaga taatgagacc tgcatatgct attgaatatg attgtgttga cccaactcaa
     5041 ctaaaaataa gtttggaaat aaagggtgtt gagaacttat ttagtgcagg gcaatttaat
     5101 ggaacttctg gatacgagga agcagcagct caagggctta tggctggtat aaatgcagtt
     5161 agaaagatag aaggaaaaga gccatttgta ttagaccgtt cagaagctta tataggtgtc
     5221 ttattagatg accttgttac taaaggaact aacgagcctt acagaatgat gacatcaaga
     5281 gcagaatatc gactatattt aagacaagat aatgcagata tgagattaac tcaaaaaggt
     5341 tatgatatag gattagttaa aaaagataga tatgaaaggt ttctaaataa aaaggctgct
     5401 gtagagaaag aatttgaaag actaaaaaat gaaagagtta caccaaaaga agtaaatagt
     5461 ttgttagaag aaaaaggagc aacaccaata aaggttggaa tatctctata tgaattttta
     5521 aagagaccag aggttactta cgaattgctt gaagaattag ggaaaggcgc tggagatgat
     5581 gtttcaagag aagttaaaga acagtgtgta ataataacta aatatgaagg ttatatagag
     5641 aaacaattga aacaaataga ccaatttaaa aaacttgaga ataaaaagct ggatgagaaa
     5701 ataaattatt catctataga aggtcttaga ttagaggcta gacagaaact agatgatata
     5761 aaacctattt caatagggca agcatctcgt atatctggtg tttctccagc tgatatatca
     5821 gttttactta tatacctaga acaaatcaga agaactagag gtggcaaagg tgaataatat
     5881 agaacttctg aaaaacggga ttgaaggttt taatatagat acgaatgaca gtatgttaga
     5941 aaaatttaaa atctatagag agatattagt tgaatggaat aaaaagatga atcttactgg
     6001 aatagaagat gaaaaagaag tgtatataaa acatttttta gactctattt cagctgtaaa
     6061 aaatggatat ataaaaaatg ggatgtctat aatagatgtt ggtacaggtg ctggttttcc
     6121 tggaatacca ttgaaaatat gtttagatag cttagaactt actttgttag attcgttaaa
     6181 taaaagaata aactttttag aggaagttag tagagtacta gagttagata atattacatt
     6241 tatacatgga agagctgaag attttggtaa aaatgaaaac tatagggaaa aatatgatgt
     6301 tgctacggca agagctgttg ctggattacc gattcttatg gaattttgtg taccatttgt
     6361 aaaagttgga gggtatttta tttgcttaaa aggtccgaat gctaatttag agctagaaga
     6421 atcacaaaag gctattgatg ttttaggatt ggagtatatt gaaaaaatag atgtagagtt
     6481 accagaggta gatttaaatc ataatatatt agtttttaaa aaagtgatgg aaacaccagt
     6541 taagtaccct agaaaagctg gtaaaccagc caaaaatcct attaagtaat gaaaaattta
     6601 ttattggtaa gcttaattat ttctcaggaa ataattaagc ttactgtaaa aaaacaaaaa
     6661 taaaggaatt attaaagtag atgcagaaat aaatttgtat gtacatatag agaacaaaaa
     6721 taatttatat ctaaaataag agaggtatgt tatggaagat aaaagagtta tggagatacc
     6781 tatagaagat gtagttccaa atccatatca accaagaaaa atattttcac aagtttcatt
     6841 ggaggaattg agcaattcta taaaggtata tggtataatt caacctataa cagttagagc
     6901 aaaagatggc aaatatgaac taattgcagg agaaagaaga ttaagagcag ctaaattagc
     6961 agagcttaaa accataccag caattataaa taatatgaac gatgaatctt ctgctgttct
     7021 agcgttatta gaaaatttgc aacgagaaga cttaaatttc attgaagaag caattggtta
     7081 tgaaaactta ataaaagaac atgcttttac tcaacagcaa ttagcagaaa aattgggcaa
     7141 aaatcaatct actgtagcaa ataagcttag aatattaaag cttccaaacg atataaaaat
     7201 gaagctaata gagaataatt taacagaaag acatgctaga gcatttctta aactaccaag
     7261 tgaagattta atgcaaagtg tgttagacaa agtcataaaa aatgaattga ctgttaaaaa
     7321 aacagaaaaa ttgatacaag atgtattaga agaaactaaa gttcaagaag agcctgataa
     7381 aaaacagaat ataaagggtg ctatgagtat aagaatttac ataaatacta taaagcaggc
     7441 ctttgatgct atatcaaata caggaattga tgctaagtat aatgaaatag ataagggaga
     7501 ttatatggaa gttgttgtaa aaatccctaa aaaatagaga ttactgctta ccagtaatct
     7561 ttttttgtaa tataagcctt gtaatataaa ttgatttata gacattattt atacttaaaa
     7621 aacacctatt caacatttaa aattaattta attcttaaag gggggaacat catgggcaaa
     7681 gttatagctg tattcaatca aaagggtgga gtcgggaaaa ctacaacaaa tgtaaattta
     7741 agtgctagct taggaacttt gggaaagaaa atattagtat tagatttaga cccacaagga
     7801 aatacaacta gtggatatgg tattaataaa aacgaagtag aaaatacaat ctatgaaatt
     7861 atgttagatg ggttacatat taaagaagca ataataagta ctgaatttga gaatgtagat
     7921 gtagttccat cagctactga actatcaggt gcagagatag agcttacatc taaaactaat
     7981 agagagtata ttttaaaaaa ttccataaaa gcggtaatag atgaatatga ttatatattt
     8041 ttagattgtc caccctcact ggggatgctt acaataaatt gtttaactgc tgtagatagt
     8101 gttttaattc caatacaatg tgaatattat gctttagaag gtgttagtca attaatggag
     8161 actataaaat tagtaaaatc aagattaaat gctgatatag aaattcaagg tgttgtttta
     8221 agtatgtttg atggaagagc taatttatca atacaagtag ttgaagaagt taagaaatac
     8281 tttaaaggta gtgtatatac aactttaatt ccaagaaatg taagacttgc agaagctcca
     8341 agtcatggaa aacctgtaat ttattatgat aaaagatgta gagggtctgt agcttatttg
     8401 gagttagcag aagaatttat agatttagaa gaggaggaat ggtagtatgg agaataagtc
     8461 taaaagaagt aatagattag gtagaggtct tagtgcattg ataccagaaa ttaaaggaga
     8521 aacttcagaa aaagaaatag tcaacataga tatagacaaa atatatccaa acgaagttca
     8581 acctagaaag caatttgatg aagaaaagat aaaagtattg tcggactcta taaaaaatta
     8641 tggtgtttta cagccaatag tggttaaaat ggatgaaaat aataaatata tgataatagc
     8701 tggagaaaga agatttagag catcaaaact tgcaaataaa aatcaaatac ctgctataat
     8761 taaagatata gatatgaaag atataatgga gatagcctta attgaaaact tacagaggga
     8821 agatttaaat tcaatagaag aggctctagc atataagagt ttaatagagc attacaatgt
     8881 aactcaggaa gaaatatcag aagctgttgg gaagagtaga cctcatataa ctaatacact
     8941 aagactttta aatcttggac aagatgttat agaaatgata gacagtggta gaatcacagc
     9001 aggccatggt aaggctttat tgagaattgc agataaagat ttgcaacttc aaatagcgaa
     9061 aaaaatagaa gaagaagaac tttctgtaag agaagtcgaa aatatagcta aaaaaatatc
     9121 tgaaaataaa caagaagaac ctaaaaaatc taaacctaag gatgtattta ttttagatgt
     9181 tgaagataaa cttagaaata tatttggaac aaaggtgaat atttctaaag gaaagaaaaa
     9241 aggtaaaata gagatagaat actataatga tgatgattta aatagtattg tatcaatgct
     9301 tcttgaatag aacaaaaata taaaacagag aggaagattg ttatgattta tttagataat
     9361 gctgcaacta catatccaaa accagaaaga gtgtataatg ctgttttaga ttgtatgaaa
     9421 aattattgtg caaatccagg tagagcaggc cataaacttg ctatgagagc tgctagggag
     9481 atatatgata caagagagaa tatagctaag ttgttcaatg taagtaatcc tatgaatata
     9541 gtatttacat ctaatgcaac agattcttta aatttagcga taaaaggtgt attacaagaa
     9601 ggagaccatg taataacaac gtctatggag cataattcag taataagacc tataaaagca
     9661 ttggaaaaaa ggggtattga aaatactgta gtaaaatgcg attatgaggg atttttagat
     9721 tatgaagatt tagaaaaatc aataaagagt aatactaaac tgattgtaac aacacatgcg
     9781 tctaatgtat gtggaacttt aatagatata aaaaaagttg gagaaatagc taaaaaacat
     9841 aatatactat ttttagtaga tgcatcacaa acagcaggtg tttatgatat agacgtaaat
     9901 gaatgtaata tagatatgtt ggcaatgcca ggtcataaat gcttatttgg acctcaagga
     9961 acaggtatat tatatgtaag agaagggttg aacttaaata tattaaaaga aggtggaaca
    10021 ggtagtaaat cagaagaaat agttcaacca gagttattcc cagataaata tgaatcagga
    10081 actcataata ctccaggtat agcaggtctt aaccaaggta tactatttat atttgaaaga
    10141 ggaatcaaca atataagaca acatgaagaa gaattatgtc aatatatgat agacaaatta
    10201 gaggaagtac cagatataaa gatatatgga ccaaaggata gtaaaaaaag agcatcagta
    10261 atcgctctaa atataggaga tatggattct ggagaggtta ctttcttatt agatagtgat
    10321 tataatatag cgactagatc aggaatacat tgttcaccac tagcacatac cactttaggt
    10381 actttaaaac aaggtgcagt aaggttcagt ataggatatt tcaacacaaa agatgagata
    10441 gataaagcag tggaagcttt gaaaaaaatt tcaaaaaata aatagcatga gatttaataa
    10501 tatagaaata aaaaatgggg atgtttcaaa ataaaaattt gaatagagta ttgaaatttt
    10561 tatgtatatt ctttaaataa aaacttcaat actctattaa ttaaattact aattttgcta
    10621 attttcataa aatctgttaa attactcata aagttatata ttttcaaagg ctcaagtctt
    10681 gtatgaaatt acttgtttta attttttata taactatgac ttttgcaatc tttttagttg
    10741 aaaagcgcct tcaatcatac taaaataata atattacaat gctaaattat tttatcctct
    10801 attataatta taaatacaaa ccggtgtgac tcatcttatg aaaaaagtat tgttcagcgt
    10861 tgataaaact gacaattaag taataaaaat attatactaa ttttactttt taaaaagact
    10921 taaaattaaa aaaacccttt tcttttgtat aacttacaat atgttctttc attttttgaa
    10981 ctgcaaaaga atcatattgt gagttgggat aagctaaact aattacacgg taatgctctg
    11041 gtacaaatgg gtatgtatca acattgccaa cacaactttt ataaattagt tctgataaaa
    11101 tagcaattcc aaaccctgct tctaccatgg caacaatagc actatcagta tctatgtgaa
    11161 attgagagta cacattaaga tgatattcac gaagaatttt ttctacttcc tggtcataat
    11221 cttttttagg cataacaaag gacatatttt ggatatcctc tattgtaaca tacccaggat
    11281 tacaaggtac aaatcctttt ggaaagacac aaatgatacg gtctttatga agcggaatag
    11341 attcaatatg tggaggaagt actgtgttgg atataaaagc taaatccaca atagaagttt
    11401 caatccattc taggatatta atatagttcc cttgaagaat atgtatttca atattaggat
    11461 gtttctgccg aaatgatttc ataatctcag ggatccagtt tattgtaata ctattaaaag
    11521 ttccaatacg aataatacca ctttctgaac gtgaaatacg attaatttct tgttctaaaa
    11581 tttcgttact atgtaataaa atacgaatat gaggtaaaat tttttcagct tctcttgtta
    11641 aactaatacc atttctattt ctaataaaca attgaaaacc tacatctctt tctaattttg
    11701 caatcgcatg acttacagct gacggagtta agtttaaaat acttgctgct tttataaaac
    11761 tattttgctc taaaactgtt tcaaaaatag tatatccata aattgacata tgatttactc
    11821 ctttccttta gatgagtaaa aactcatcta aaacgataaa aagattcgct ttattttata
    11881 ataataatct actattatta aattataaat acaagtgatc atttgcattt tttactcaag
    11941 ataataaaaa tagtaccacc caaaatacaa gaattaaaat attatttctg tttaaaagtg
    12001 agcggaaacg gaacctttgc tcatctaatc tcatataatt acagacaaat atcaacttga
    12061 taaaaaggag gaactaatat ggtaaaattt gcaaaaagaa tggaaaatat gaaaaattca
    12121 gcaaatattg ttcgtaatct ttttaattcc atgacagatc ctgaaattat atcttttggc
    12181 ggaggtgcac cagcaataga ggctttgcca gttgacattg taagagaaat ttctaatgaa
    12241 gttttagcaa aaaacaaaag agggattgaa gccttacagt acagtcaccc atatggttta
    12301 aaagatttac gtgaagcagt tgctcagaaa ttattagaac caaaaggagt acatgcaagt
    12361 ctagaggaca ttatgattgt aaatggtggt ttggaaacaa tgaatctgat ttgtcaggta
    12421 ttcattgatc caggagatgt cattttagta gaatcaccaa catttgtaca ttgtgttgaa
    12481 atttttgaaa tgtttcaggc aaaatgcata gcagtagata tggatgaaca tggaatcatt
    12541 ccagaagata tagaaagaaa gattcaaaaa catcacccaa aaatggttta tgtaattcct
    12601 acttttcata atccaactgg caggacattg actacagaac gaagaaaaat attggcaggt
    12661 attggtagta agtatgatgt tttgatttta gaagatgacc cttatcgtga tttgagatat
    12721 agtggagagg agttacctcc tattaaagca tttgatcaaa caggacatac tattttagca
    12781 aatagttttt ctaaaatctt ttctccagga gcaagacttg gttatgtttt agctgaacat
    12841 gcaatcattg aacgattact tgatgcaaaa acagcaacta attctcatac atcagctatt
    12901 tctcaagtat tgtgtgcaga gtttttcaat cgtggttact ttgatgaaca cttgaagaat
    12961 attcgagata tttatagaga acgcagagat gtgatgattg aaagcattga taagtattta
    13021 ccaaaaggta ctaaaagggt atttccagat ggtggtttat ttacttgggt agagctacct
    13081 gaaaatatca atacaactga attattgaaa gaagcggtag aacataaagt agcttttgtc
    13141 gcaggagaag gattctttac agaaggtggt ggaaaagggc gaaattgtat gagaattagc
    13201 tttggcaatg tacctcctga aaaaattaga attggtatgg aaaggcttgg acaactaatc
    13261 gcttccaaaa tgtaaataat tacttatata aaatacaact ttatatttta agatttataa
    13321 tataaccaaa ggaggaatac ttatgacaaa taaagtacca aacttatgga atcaattgga
    13381 aaacttaaaa aaatcatata aatgggtgga tttgagtcgt gaagtaagtc ctgagacacc
    13441 tcattggaca ggctttcctg caatggaagt agaacctttc tacttattgg atagaggaga
    13501 tggatttaat gtaaataaat atacactagt aactcaatat ggaacacatg tagatgcacc
    13561 aattcatttt gttgccgata gaaagtattt ggatgaatat acgccaatgg atatggtact
    13621 tccactttgt gttattgata agagtaatga ggttgctaaa aattcagatt atgtagtaac
    13681 tgttgaagat attcaagcat gggaaaaaga atatggtaat attccagagg gtgcatttgt
    13741 ggcatttcgt tcagattggt caaaaagacc tgatgattct ttagataatt atgattcaga
    13801 tggtaataag cattttccag gatggagtat agaagctctg caatatatgg ttgagaagag
    13861 aaatattgct gcaattggac atgaaacttg tgatacagat gcaccaattt tatcaaatgt
    13921 aagtggatat caggcagaaa catatatttt aggagaaaat cgatatcaag ttgaattttt
    13981 agtaaatttg tctgagttac ctgcgatagg agctattatt tttgtaacat tcccaagaat
    14041 acacaaagca tctggtttta ctgcccgttg ttttgcaatt tgcccataag gttttaactg
    14101 ttagaaggga gggggagaca gatgagtaaa aaagcgatgt ttgtttggtg tgttggctta
    14161 ggtctactag atggcatcta ttgtattctt tgttcctact taccccatat acaaaactat
    14221 atgtggattg gttttatttc tcttccaatt tacttttgtg gaggtgcaaa attagaagag
    14281 atgccaaaat atttttgttg tgcagcaagt ggtatatttt ggggtgggct aacattagtt
    14341 gtattagaca taggatttat atccaatata catttaaata tgcttgtgtt cgtaacaatt
    14401 gttgtatgca ttgcttgctt tgtacatcta ggtgtattga ctgaaaacgt atggaaagga
    14461 ttgttctcaa gttgcccaat gatgtttggt ggatttgcag caattttttc tcaaggatta
    14521 gcggagtggt tttgggtact tgtaacactt acactagggt tgatttttgg ttgtatcatg
    14581 gcttcaattt caacacctat cacaaaatgg attgataaga attgagataa agtagctatt
    14641 caataatttt taaaaagtta atttatttat gaaatataat ggtatattat ttcaagatgg
    14701 tttaaaagga aaagtttttt aatacttttc cttttaattt aaaatataaa tacatttgcc
    14761 acataagtgc ttctttaaaa tctagtatta tctttactag atataggagt aagttgtttt
    14821 accaaattag ttttatatga agatatttac aaaattttag gaggaaaaaa caatgaaaaa
    14881 tactatacaa acttttaaga atgctaaaag tgaaggaaaa aaactatcta tgcttactgc
    14941 ttacgattac tcaatggcaa aaataatgga tgagtgtaat ataaatggtc ttttaatagg
    15001 agattcatta ggaatggttg taaaaggcga agaaaatact ctctctgtta ctatagatga
    15061 aattatatat catacaaaag ctgtaaaaaa tggggcaaaa aatgctttaa tagtaagtga
    15121 tatgcctttt ctatcttacc atgtttctat agaagatgct gttaaaaatg ctggtagact
    15181 tgttaaagaa ggaggagctc atgctgtaaa attggagggt ggagcaaatg ttataaagca
    15241 aatagaaagc attgtaaatg ctcaaatacc tgtaatgggt catttaggac taactcctca
    15301 atctgtaaat tcatttggtg gatttaaggt tcaaggaatt actagtgaag cagcaaaaca
    15361 acttattgaa gatgcgaaat taatagaaaa agcgggggca ttttctattg ttcttgaagg
    15421 tgttcctgcg aaaatatctg agataataac taactctatt tcaattccta caatagggat
    15481 aggagcagga attaattgtg atggacaaat tttaatttac caagatatgt taggtatatt
    15541 tgaagatttc gtacctaagt ttgtgaagca atatgcaaat gtaggagata taatgaaaga
    15601 ttcaattaaa aattacatac tagaagttga aacaggggca tttccacaag aaaaacatag
    15661 tttttcaata aaagaatctg aattagaaaa attatacgaa gattaaggat gttataagct
    15721 gtcatatata acaatttatt attatattta gagatggatt gtccatcttt tctggataaa
    15781 aatttatcca gttttatgat tataataacc aattgtaaaa tatatctaaa caatatatta
    15841 tactatgtag atttttcaat gaaataacat cagatattta ctgaatattt ttatttctta
    15901 ttttttagag gaacttcttt taatttaaat ataaaaataa tgaatgtgtt tatatagtaa
    15961 ctgtatatat taatagttta aaaggacact attcattcgt aaaaacttaa attgatgatt
    16021 ttattaaaaa atatgtagaa gatattgtag gtttatgtct tcatagaata atgattatat
    16081 tatatgaatg tgatttagca taaaactaga tatattaaag ttgagtgaga agaatcttga
    16141 acaaagttct tcttaccaac tttaatatat ttctattata caatctattt tttatcatta
    16201 ttgaaattgt ttacaacttg atttaactca attaatatac ttccaacttc catatagaga
    16261 attttactat aaacacaagt aatacaatag gaatgtaatt atcacttgat ttttttattt
    16321 tttaaatccc ataacaaaac attaagtgaa actattggag ttaacaattg ttgtagtata
    16381 aaagctatta agttatctat atattatctt cctaaaaaag ttttcttaac actttaatta
    16441 ttattcaaaa act
//
LOCUS       DAFKMX010000091        15966 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFKMX010000091 DAFKMX010000000
VERSION     DAFKMX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.091"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(67..879)
                     /locus_tag="LZB08_002228"
     CDS             complement(67..879)
                     /locus_tag="LZB08_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7315726.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTIGLVLQK"
     gene            complement(1249..2151)
                     /locus_tag="LZB08_002229"
     CDS             complement(1249..2151)
                     /locus_tag="LZB08_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7315727.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(2201..2662)
                     /locus_tag="LZB08_002230"
     CDS             complement(2201..2662)
                     /locus_tag="LZB08_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBM7315728.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2714..3169)
                     /gene="def"
                     /locus_tag="LZB08_002231"
     CDS             complement(2714..3169)
                     /gene="def"
                     /locus_tag="LZB08_002231"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBM7315729.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVGGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3141..3404)
                     /locus_tag="LZB08_002232"
     CDS             complement(3141..3404)
                     /locus_tag="LZB08_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315730.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3501..4217)
                     /locus_tag="LZB08_002233"
     CDS             complement(3501..4217)
                     /locus_tag="LZB08_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315731.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4214..4897)
                     /locus_tag="LZB08_002234"
     CDS             complement(4214..4897)
                     /locus_tag="LZB08_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315732.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(4894..5739)
                     /locus_tag="LZB08_002235"
     CDS             complement(4894..5739)
                     /locus_tag="LZB08_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315733.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5853..6443)
                     /locus_tag="LZB08_002236"
     CDS             complement(5853..6443)
                     /locus_tag="LZB08_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7315734.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            6732..6965
                     /locus_tag="LZB08_002237"
     CDS             6732..6965
                     /locus_tag="LZB08_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBM7315735.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            complement(7196..9583)
                     /locus_tag="LZB08_002238"
     CDS             complement(7196..9583)
                     /locus_tag="LZB08_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBM7315736.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPSPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVDDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(9775..10530)
                     /locus_tag="LZB08_002239"
     CDS             complement(9775..10530)
                     /locus_tag="LZB08_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBM7315737.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            complement(10555..11706)
                     /locus_tag="LZB08_002240"
     CDS             complement(10555..11706)
                     /locus_tag="LZB08_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBM7315738.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            complement(11906..12646)
                     /locus_tag="LZB08_002241"
     CDS             complement(11906..12646)
                     /locus_tag="LZB08_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBM7315739.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            complement(12718..13707)
                     /locus_tag="LZB08_002242"
     CDS             complement(12718..13707)
                     /locus_tag="LZB08_002242"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBM7315740.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHEKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            13967..15220
                     /locus_tag="LZB08_002243"
     CDS             13967..15220
                     /locus_tag="LZB08_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBM7315741.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNDFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            15317..15541
                     /locus_tag="LZB08_002244"
     CDS             15317..15541
                     /locus_tag="LZB08_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBM7315742.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
BASE COUNT         5340 a         2611 c         1676 g         6339 t
ORIGIN      
        1 agcttttatt atgttatttt attgttaaag gtttcaagtt tttatttatt cattaaaaaa
       61 aaattattat ttttgtaaaa ctaatcctat agtcatacta tcgtcagaat atgcttcacc
      121 cttaacatta ctaaaaaact caacagactt aaatccaata ttttcaactt ctaataagaa
      181 actttctttt gaaaaatagt gattccaaac ataaaacaca ttttcatctt tttcggttat
      241 tacaagagtt tgttctaatg tattataatc attgtattta caattatctt gtagacacat
      301 atatttttca ttactccaaa atcctccatc ttcatttatt tcccaatatt taatttcctc
      361 aaaattattg tatttattta ttgtaaatac atctaatatt aattttcctc caggttttaa
      421 actatcataa attttttcca ttaataacct tctattttca gttgacaaag ctccataatc
      481 acaataaatt aaagtagcca aatcaaattc ctcattatag ttcatattta aataactttg
      541 aaacaagtaa tttatgttta aattagtctc cttatttcta ttttgtgcat agttaataga
      601 acgttttgag aaatcaattc cagttacctt ataacctttt tgtgcaaatt tctctgcata
      661 taaacctgga ccacaaccta aatcaagtaa tttaggataa ttagctggat ttgcaactgt
      721 aactatccaa tttacagaat cctcgataaa gttaaaattt ctacttgctc cttcaaaatt
      781 agtgtctaag tgtgctttta ataattgctt tgaaatatac tcatcatccc aaaaattaat
      841 ctcactttgt ttataaagct ctggtctttc taaatacata cataatttgt ttaacaaaac
      901 ataccactcc ttaaaattta tttttaaaat aaaaaaacca cagagtttac tctgtggttt
      961 aacttcaaaa agatgagttt aaataagagt aaaatagaac caaatatttt taagcacaac
     1021 aataaaacta tagctttact ttgtttgctt attaaatttg attttattca ctcttttcca
     1081 gcaatactta tatcaacgat tttatgatat caaatatcac cgaacaacat catcctttca
     1141 tatttttatt ttatgaatta attatatatt taaattttat atttgtcaat aaattttaat
     1201 tttaataaat tttaatcatc atagtaaaaa aaattttttt atataaaact aatcaaaaaa
     1261 agatatctgt acatctcctg agttttttaa cttttccttc attattttag agtaactgct
     1321 tgatagagaa tacttatatc taagttcatt aaccatttta tacaattgat ttttatactc
     1381 cctattaagt gaaccttcac tatatagtat tgataattta tcaaataatt caggaaactc
     1441 ttttttaaca aagtcaaaaa aaacacctct agttataccc cttaagtata aagtcccagg
     1501 taaaacataa tgtacattac attcttttgc atgtctaaat agcgaattta tattatcaaa
     1561 atcatctgta atatatggta taattggcat aatatgtaga ccaactgatg cgttagtttt
     1621 tctaaattct tttaacattt taaatcgtct cattgaatcc acaccatttg gctcaataag
     1681 cctttgtgta tcttcattta ctgtggtaat tgtagatgct acattgatat atgtaatcct
     1741 agatagctta tcaattaaat cgtaatctct taaaattaaa tctgactttg tagaaataat
     1801 agctggtgtc ttgtatttta tcagcaggtt taatatttct ggcataattt tatagtcagc
     1861 ttctattggt tgataactat ctgtaactcc tcctatattg attacttctt tcttccattt
     1921 actgctttta agttgtctct caagtttttc tactatattt gtttttacat aaatctcttc
     1981 aaaataatta ttggagttga tgtacttatg agagtaaata gcataacaat atttacatcc
     2041 atgctcacat ccacgataaa tatttaaatc ccatttatat ggcatactcc tttttaattt
     2101 attacaagct atttcacaat ttattggaat atattttatt ttattataca aataatcacc
     2161 tctttatata caataatact ataatgttaa tctatagtaa ttaaaacatg atatttccag
     2221 accacaattc ataatcttta ctatatttac aaagtaatat attccattca tttattggta
     2281 ttcctccaca tgttgctttt tcttctaaat tatataagca ttgctttaaa tacattttat
     2341 tatgccaatc atcatataaa ttttctaaat ttaaactatg ttcattagat atatcatttg
     2401 caaaatttct atttttccat tttaatatac ttgtatattt aagttcatta ggtttataat
     2461 ttctttttaa catttcatca tgtacaattt ttgtataaaa tttaaattca tctatactat
     2521 aattcaatac tttattcact aatagatggt ttggagtacc ttttttatct atagaacctt
     2581 ttatagcaag tagctctctc cattgactta ctaattgatt tttaggtaat acatcaatta
     2641 aatccttatg ccataatctc ataatttaac tccttactat aaaatatttt ataaattcaa
     2701 taaccaatct atcttacaat tttacatgtt caataatttt atctgtaaaa actataccat
     2761 ttaaatggtc tatctcatga caaagacatt ttgccataaa acctgaacct ttaatttcta
     2821 ttttctcacc gtattcgttt agagcttgca ctgtaacttt ttttggtctt tttaatttac
     2881 cccctacttc aggaaaactt aaacatcctt caacaacaat ttgttcccct tcttgtttaa
     2941 taatttcagg attaacaagc ttaataagtc cttctcctaa atctataaca actaatcgct
     3001 ttaatactcc tacttggcat gcagctaatc ctcctccatt tggagtattg tacatggtct
     3061 ctgccatgtc atttaatatt tctcttatct tattatctac gttttctaca tacttactct
     3121 tttttcttaa aatttcatca tcaaatgttc ttatctttct tatagccata atattctccc
     3181 ctacttatct gtgctcttga ttttctttca ctttcttcaa ataaaaatac actttcaatt
     3241 ggttcattaa aaattcttga aatatcatat gccaaccata ttgaaggttc atttttacca
     3301 gtttctatag catttatagc ctgtctagat gttccaacta atcttcctag ttgttcttgt
     3361 gtcaatccta atttttctct taatatcttt attttatttt tcatatttgc tccattcatg
     3421 taaggttaac tttacatctt aaatgtaaca tttttcaaca ttactgtcaa gtcaacctta
     3481 catttatata ttaaattttc ttatttaact atttttttac taaacttttt aaataataat
     3541 aaaatccata ttaatgatat aacaattgaa ataaacatat agacataatt atcttcctta
     3601 aatgccttcg atatccaaaa agttggtaag aagaacagaa tatattgtgc tttatttaaa
     3661 ataaaaaatg gtgctaaaat acccatcata aaaacacctg agaatttcgt aattaccatt
     3721 ccttctagtt tatttgatga taaagaaatc actaatattg atataataat tccttgtaat
     3781 aaaactaata aagaaattga taagtttaac aagagagaaa ttttagttag agaaaaaaag
     3841 ataagtagaa tcatagtaat aataaatgct aagatacctg gtattccaaa tcgtgaaata
     3901 agataaccag ttttaccaag cggagttact attaaatatc ttgaaatgct gtcatcaatt
     3961 tctcccaata tgaccattgt agaagcaaaa aagaacataa aaggtgtaat aaaagctaaa
     4021 agtaaatcaa acatcaagaa atacggctct aaatttaatt gataataaaa cttgttaagt
     4081 gatatattct gtattaatgg tattccaaat tttataaata ctccacataa tattggagaa
     4141 acacatacta taaaaagcat agcatctctc ttaatttgtt caaactcttg tttaaacgca
     4201 tttataatct ttatcatagt tttactccac ctaccttttt ccaactgcta caaataaaat
     4261 aataagttat aaaataaatt attattataa ttgatattaa aattaaaata ttaatcatta
     4321 taaaattttt atctccacta ataagagaaa tgcatatatt aaaagggtac aaatttgcat
     4381 atgattttgt gtcaagaagt acattcagta ttggtggaat aaagcatata atctcaatag
     4441 gaatacttaa aacaataaac tgattcaaac tagatgcttt acttgcaatt atcaatccaa
     4501 gcaaagaaaa tataatagaa gaaaaaaagg tacctatagt tgatattata atattatcta
     4561 aatttaatgt aatagcaata aacatagcta caatactaga tattactcct aatgaaatta
     4621 cctttgataa aatatactca gatactttta ctggagatac agctaaggaa tttaaaaccc
     4681 tttgactttt ctcaagaagt acaatagctc ccataaaaaa caatcccatt gctgctgggt
     4741 cagaataaat cataattata gctatttttt ctctcataaa agttggaaat acgttgataa
     4801 tacaaatata tataatactt aaaaaaagat ataaaaaata aaaaccatac ttaaattgaa
     4861 actttatatc tcctaaaata agatttttta gcctcattgt aaattcctcc ctgttatctc
     4921 aataaaaata tcatttaatg ttggttcact gctatgtatt gataaaagtt tatttttctc
     4981 aataagcata ttcaaatttt tatcattagc agtattgttc agaaaacatt cagaagtttt
     5041 ttctccatta tcaaagtaag tatatctaac tttaatagct ccttttgaca taataagatt
     5101 atgtggtgta tctaaagcag atattttacc atttacaatg aacgctactc tgtcacaaag
     5161 ctctgttgca tctaacatgt tatgagttgt taaaataata gttttacctt tagatttttc
     5221 tgataaaatt atatctttca taaccttgct atttgatggg tcaagaccac ttgttggttc
     5281 atctaaaaat aaaatatctg gattatgtaa taaagctttt ataaaattta atcgagattt
     5341 catacctttt gaatactcag ataccctttt atttgcttca ttttctagtc ctactgattt
     5401 taataactca tcaatagata gtagtttttt agaatataga gacccaaaat attttaaatt
     5461 ttctattgca gtcaactttt catataaact aggaaattca aaatcaactc ctatattttc
     5521 ataaaattta tttgagtgtc ttttactctc tactccattt acaatgacac ttcctccata
     5581 attagttatc attccaatta gaatcttttg taatgtagat tttcctgcac ctgatggtcc
     5641 taaaaatcct agtatttccc cttttcctac tccaaagtta atattttgta aaaaatctct
     5701 atctgtataa ctaaatgata aatcatcaac tttaatcatt caatatcacc ctttcaaatt
     5761 tttgactatt ttgactatat ggtcaataaa atataatttt tttgtttgtt aataattttt
     5821 aataaaagtt attagcaaac aaaatttaaa ttctattcca taagttgtat aactaatccg
     5881 tttatcatta atcttaaagc atcttcaaaa caattacttc caacctcgcg cttatatagc
     5941 atggtaagaa aaactgctct aaatgctcca ctaaagcttt ctatgtcttt attttttgca
     6001 tttggtatgc aagatataat ctctccaatt tcaaagtcat catgtctaaa gtgttcttca
     6061 attatagaat ctggtaactt tctcaccaat atctcaaatt ctccatttat catgagattt
     6121 aaaattgaag tactatcaac catcctatag aatttcataa atatatctgt caattgttca
     6181 catgtaatac tttcatctac tctatttatc tcatataaga tttctttttg tatagagtca
     6241 tgtatatctc ttaatacctc aaaaaagagc aattcttttg attgaaaaaa tagataaaaa
     6301 gttccttttg gtatttttac ccgttttaca agctcatcaa cagtagtttt tctaatacca
     6361 tattgctcca tacacaatct tgcttcttct ttaagtcttt ttattatata ctctctttct
     6421 ttatcagaat atgatttagg catcttttcc ctccagtttg accaaaataa tttatatagt
     6481 caatataata ctttttctat ctaatgtcaa tagtattatt agtatattta aaaataagct
     6541 taaaaacttt cgaaaattct gaattttata tagcattttt tattatctag tgttataatt
     6601 aaaatataaa taatatatta ttctaatttt catatctatt tgcttatttt ttaacttagt
     6661 ttctaataga agtaatcata tttatctttt taaaagtttc aactttaata aaaaataaag
     6721 gagttgattt tatggctact actacaaaaa accgttttta cgattatgtt caagaaaact
     6781 taaataaata tgttgaagta cattcttact ttagcagttg tcctctatat ggtgaaatta
     6841 tagaagcaga tagcttatca attacacttt gctcaagata tatagatgaa tctaatccaa
     6901 ctttaaatga aacttacaca ttatatttac cattaagttc aataatatct attagaacac
     6961 tataaaaagt taattaaata tgggttattt atatataaaa tgtaagattt tctatatttc
     7021 tatataaagt aacccttttt tatgttttta aacttatact acataaattt aaagctgaaa
     7081 acaaaacttt acaatcattg attatcttat ttatcaataa tttgaagtaa ttattttcag
     7141 cttttattaa gataagttac taatacacac taatatatgt taatacttaa gtactctatt
     7201 tagcactttt taattttact aactctttaa atgcattttt atttgctcct ccaccaactt
     7261 gagttacttt ttctaaagtc ttatttttaa gaacttcttt ttggctatca gctaatttat
     7321 caccaactaa aattactggt gacccagttt ttgctcctaa aggaccaact gccaatccat
     7381 ctattaaatc attttgattc ttgattccat ttttaactac aaatgcattt tttaaagtat
     7441 cttttttata aaaattatct attatctttg aattagtttc gtctctacta ctaccagaca
     7501 atctagttgg attttttaac ttactttcta gattgtttga tagggatgct gttcctccaa
     7561 taatataaga tttctctata tttaatgaat ttagaaattc atcagctcct tgcaattcgc
     7621 tcttttcatt tgcaagtatg attggtatgt tattttctgc tgctgcagcg cctacactaa
     7681 ttgcatcagc taatcctttc actccattta caactgctat ttgagataca ttttttatac
     7741 catttaactt ttgagctaat aataaacttg tcttgtatct atcacttcct gatattctct
     7801 ctatcgatat atttaagctt tttaattgtt gttcaatttc tttacttata gaattttctc
     7861 cacctattag gtatactttc ttagcaccta atctctgtat ctcagcttta gtttttgaat
     7921 ttaaagcttc tttttgtgtc aataatattg gtgaatttat agcagatgta aaaggagttg
     7981 ctgccaatgc atctactatt gaactgccat ttactaaaac tacattttct gaagaattga
     8041 aaccattttg actaactttg atagcagttt catatctatc actaccaatt aaagactcat
     8101 ttgggatact ctcaccagaa gacttttctg taacagcttt tatagtaaaa ttacacggat
     8161 agaagttact atctctattt atatcaaaga attttccagt attatctatg atataacttt
     8221 cacctaaatt agcttttgct ttaaatgtat catatcctgg tacatttgac tcagaaccta
     8281 tagttgactc tccatttttc ccctctatgc tgactacaat tgcacccttt ccttctggta
     8341 tatcaaaaga atcaattgga actgttatgt atcctgaata ttgggcagta ccttctttta
     8401 attttttcat attgttttct tgaggtatac catttactac tggagcataa tatacttcat
     8461 acttagctcc tatactttca gttaagaaca ttacagattt tagagtttca tcgcctctat
     8521 tgaaatcaaa tacatttgca gctgttatct tttttgaaat taaaggtaca atacttgcat
     8581 agtcatgttg atacatcttc tcatcatcat ttggtttttt accacctttg attgaaaaat
     8641 tatctatatc agacataagt gtcttgtctt cataagatat ccagaaatat ccaccctcag
     8701 aattataatc tccccagcta ttttttatta accaagcacc attttttgat ggtctaatat
     8761 ttgcattaaa attatcttta gaatagttat catcccaacc tacaattgaa atagcatgat
     8821 ttgtattaaa actttttgtt ggacaataat atgaattttg gtcatcactt tgatatttaa
     8881 tatcttcata ataaccagat gttacagctc catactgtaa tatagcattt tttgtagaat
     8941 ttatgtcatt gcttacacac acagcatctg tcacattaaa aacagtaggt gctgtattca
     9001 tattagttgg tttttttgct ccttgtgctt tagatactct accattgtat gggatatctt
     9061 tttctaattt aggacctgac caagatgtta aatatcccat agcttcataa tttaaagcac
     9121 cattcatatc atctacagac catccatatt caccatctga agcccaccat cttaagtgtt
     9181 cttctgataa atcatagtct ccataattat ttaactttaa gaaactttct aaggttgcca
     9241 ttgcagcaaa atcccaacat attcctaaat cctcttgatt ttttattgat gtcataagac
     9301 ctaattttct agggtcataa gatgttggag ctgttcttcc tttactttgg atttgagtac
     9361 cttccaactg atatggactt ggtataatcc cattatattt ttctgtacgt ccatttttag
     9421 catcatttag atattgttta tacgtctcac tgtattctgc tttttttatc tgattcaatg
     9481 tattgctatt gtcagctgat acaggaattg ttgacattac taaaatagat attaaaaaca
     9541 gcgcaacaaa ccttgttact ttttttgaag taaatttttt cacgctttct cccccttatt
     9601 ttgagctaat ttttaaaatt ataatgttat atatgactaa atatcaagta tttacaaata
     9661 tacaatcatt taaatcaatt ataccaaagt tttcctttat aaataaataa aataaaataa
     9721 aaaactgaat ttatttataa taaattcagc ttctcatttt ctttttatat gtttttatct
     9781 aaatcctatt acagcagctc ctaaagctcc tattatttga gtatcttctg caataaaaat
     9841 ttttttaccc agtatttctt ctatcatttt tactagttct ttactttttg caagccctcc
     9901 agtaaatgca acttcatcta ttacctcacc tcttgctaac attgaggtgg atttatttgc
     9961 aatagattta agtatccctg ctgctacctc tccagtagaa atgttttgag ctagaagact
    10021 tacaatttca gactcagcaa aaactgtaca catactagat atattaactg gttcatatcc
    10081 tttagctaat gtatctatac tttcaatatc gcttcctaaa aggttagaag taatctctaa
    10141 aaatcgacca gttcctgcag cacacttatc attcattata aagttaaata catttccatc
    10201 tctatctaaa tttataactt tactatcttg accacctaca tctaaaatag tccttatgtt
    10261 cttgtttaaa aagtaaatac ctctagtatg acaagttatt tctgttactt tcttatcagc
    10321 aaaatccatt actccccttc cataacctgt acctaccacc ttttttatat cttttttgtc
    10381 aatttcacta cttagcaact catagacctg tttagatgta cttttagggc tccatccagt
    10441 tggtataatt attttcttaa taattttttc accatcaaat agtacaccct ttgttgccac
    10501 tgaccctgag tcaactccta tactaaacat agttcctcct acttttattt attcctacaa
    10561 catctctaca aacgcttcaa atctagttct taattgttca gtatctgatt ttgaataatc
    10621 agtttcaaga gacataaatg gcttattttt ttcttttgta acaaattctc ttattctata
    10681 actttctata ttaaatgtat gacatgcttg taaaactaca tctattacag catctacatt
    10741 atattcatct attattctat ctaataattc aatacgattg tcattattca tcatacaaga
    10801 acaacctata tttatatatt tttgtgttaa agcatcatat acatcttcta tttcttcatc
    10861 aaccaataaa tcgtttgatc taatagctcc acaaagttca tatgcaacga ctgatgcccc
    10921 tgcatcttct aaagttttaa ctattttttc gcttattcca ccaataggag aacctgttat
    10981 caatattctt ggcttatctt tttgaacagg acattcacct ttttcatatc tttctttcat
    11041 atcatctata acttttctta aagaatgttt aagttctgca cggtcaaatt taaaacctgc
    11101 ttgatataaa acgttatgta actcaaggcc tgtaagtgca gatggttgta atttaccaag
    11161 tgcataaaac tcttttaaaa gttctctttc ttcattttta tcttttatag cagttcttat
    11221 atcttcttca gttatagtta cacctaaaga tttctcaacc tcttctttta atcttatcat
    11281 ttcatctttc caaagtttat aggcaaattc tccttttgaa gtatttggca aatgcattac
    11341 atatgttggc ttaacttttg caagttcttc atacattttt ttcttaccat cacaagtggt
    11401 ttctcctatc aacaaatcag aaaaatagaa ataaggacag gtatctgtta ttgcatgtcc
    11461 atagcttgat ttaattaaag gacaaagatt tcttggaagc accctttctg catcaggtat
    11521 tggctcttca ctaacgccgc atacacctac tacagttgct ccagctgcca ttggaatttc
    11581 aactggagta aatgtacaga aaactccaac catctttttc ccactttcct taagttcctt
    11641 tgcatttata aatccttttt gtctagcttc attgaagcta tcaaattgtt ttggcaaatt
    11701 tgtcatgaaa atattcctcc caattacttc tcttcgtaat tcaatttgta tcttaagcaa
    11761 taaaaactaa atttttccat taaaatacaa acctctacat tagattctat catgctttac
    11821 cttttatcca taattgtttt tatcaatgca aaattagata catatagata tattgtataa
    11881 ctaatttttt atgttataat ttctcttata acttaagctc tcgaatcctc aatctataag
    11941 cagacacagc agtttcacat aatttatcaa ttaattttcc attcacataa aatcctacaa
    12001 atgctcctat tccaggcatt agttgtaaaa acttagctaa atctaaataa tccctatact
    12061 cttgttgaaa tgttctccaa tcaaaatcat tgatgtcttc tggtagacta tttacatatt
    12121 catcccagtt attcatcaat ttaaaagttt tgtttatatg ctgttgacta gaaaaagcca
    12181 actgtaatat atttaatata taaatccttt ctctataatc tttaacatca tatccatata
    12241 ttgaagctac atcatataaa aatttcactt taatacctaa aagcaaagga aaatcagata
    12301 aacctaagag gattcctcct gcccctgttc ctgctccttc aatcattgct gtggtcttgt
    12361 aagttttagc tttttctaaa actaattttt ctctttcttc taatgacata tttctaagtg
    12421 gtggctttgt agcatattta tatccaaaca gtacggtttt tgtcattact tttatagaag
    12481 aagtaattat ttcatgatat ttatctggca atactccatt aaatttattc tgtataccct
    12541 ttgatgcttt gtcaaatata gaaggtttct ttaatatatt tttcttccat tcttcaagtt
    12601 cttttttgac tagtttaata taaacaacat cttcataatc tttcacattt tttctcctaa
    12661 tacaatgtat ttttgtcaat ttaatagctt aatttaaagt ttaaatattt ttatctatta
    12721 aattaaacta ttcaaatact ctttagtttc tttatctgca aaactagctt ctactgcatt
    12781 tctataaatt attttatagt cttctaatcc tagtttaaaa gtatcaaata taacatttag
    12841 ttctgaactt aaatttatat ctgatacagt tctattatca gtatttatac ttacatgtaa
    12901 attatcttta taaaaagtat aaaatggatg tacctcataa gaatctactg tcttagtttg
    12961 tacattacta gttggacata attcaagcaa tattttttta tctttaacta aattatacgc
    13021 cttttcatgg tctttaattt ttaccccatg accaatccta tccgccttta aaatatttat
    13081 agcatccaat acattttctc cacttgcagc ttctcctgca tgtattgtaa ctttatatcc
    13141 atattctctt gctaatttaa aaacatcttt atattcctta caaaatccct ctttctctgg
    13201 accacacaag tctactgcta caacaccttt atttacaaat gatttccctt cttcaattac
    13261 taataatgcc tcttttgagg tcattgttct catacaacct aatattaaat tgccttttat
    13321 atcataaata ctttcagctt ctctaattcc ttcaattata ccctcaatta tatttttaac
    13381 actcatacct ttttgggtgt gaagtaatgg tgcaaatcta atttcaatat attttacatt
    13441 ttctctactt gcatcttcca aaagttcaaa agtaattctt tttaaatttt ccttagattg
    13501 cattactttt aagggtaaat caaatttttc taaatattca tctagtgaag tacaattaaa
    13561 agaaaccttt gctaattttt ttatttcatc catattattt gaaggtaaat ctattttctc
    13621 tttaatagct atatcaagca tggtttctac tctaacactt ccatctaagt ggcaatgtaa
    13681 atctatctta ggtaaatttt caaacatttt atcctcctac tgtacttata aataatacta
    13741 tcaacagatt tttaagtctt atttctatta tatcaatata ctacggtttt taattctaaa
    13801 aaagtcatat ctcattaaaa tattttaatt taatttatga ctatctaatt atatctactg
    13861 attcttttta atatacattt attaatatac atttgataat aaaatttcaa tacataagaa
    13921 tttaagttat ataattaaaa ctttgaatta caaaggagga cttattttga ttaccttaag
    13981 ttttgatata gtgcaaacct tgacactatc tatattcttc tttttaattg gtaatttatt
    14041 gaagaataaa gttaactttt taaacgattt ctgtatacct gcaccagtaa taggaggtct
    14101 attattttgt tttctaaatt tattttttaa atattttaat atagcagata tatctataag
    14161 tggaaattta atgccaaatt ttataacctt cttttttaca actataggac tcgaaataag
    14221 cataaatctt ataaagaaag ggggtagtgt attatttaga tattggattt tgtgtggagt
    14281 attggcattt tgtcaaaata tattagctat tacaatatca aagattataa aacttgaacc
    14341 tcttttaggg cttatgtgtg gaaatgtatc tatggaaggt ggtcatggtt attctgctgc
    14401 atttggactt acaatagaaa atctcggtat agaaggagca gttggggttg gattatctgc
    14461 tgcaacaata ggacttatta tgggtggtat attgggatgt cctgtagcta aatttctaat
    14521 aaataaatat aaattaaaac catcatcaaa tatagacttg tcgattccta gatataatag
    14581 aaatctaaag agatttggaa gtaaattttt tagaaataag aataggataa aaaataataa
    14641 cttctccgaa acaataacac catctgtgtt tttggaacag gttcttctca tatttatttg
    14701 tataaataca ggagaaatta taagtagatg cttctatata acgtttaata tactattacc
    14761 ctctgttgta acttgtatgt tttcggctgt tatttttaga aatttaaatg ataagataaa
    14821 tatactagaa ttaaatttta aacttataga ttttttaaaa gaactttcat tgggaatttt
    14881 tttaacttta tcacttatga atatagattt atttgaatta tctacattat tacctcctat
    14941 tctcttaata gtaacttttc aagtaatctt tataattcta ttttcaatat ttatttgctt
    15001 tagagtactt ggaaaagatt ttgattctgc catcataata agcggtttga ttggacatgg
    15061 aataggagct actccgaacg ctttggcaaa tatgagttct ttgacacaaa aatatggtga
    15121 ttctccaaaa gcctttttgg ttgtaccatt agtaagtggt tttttgctag atgctattag
    15181 tatcccatgt atactatttt ttataaacat actaacttag tatgtttatt ttttgttata
    15241 tattgacaat ttgctctttt tgttatatat tataaatata aagtttgata atgattttca
    15301 ttaggaggtg ttaataatga ctgtttacaa cttaaaactt ggacaaaagg gaattataga
    15361 caatatagct ggaaatgaaa aacttatgaa gagacttcta gccttaggac taatagatgg
    15421 cactgaagtt gaagtgaaaa agatagctcc tcttaatgac cctatagtga ttagatttag
    15481 agggtttgac ttagctatta gaaaatctga tgcaaaaaat ataaatttaa aaaataatta
    15541 atatataata aaaaagagat tttaaatcta ttttcaggcc atcaacattc tatgtgttga
    15601 tggcttttta aattcaatca tgttgtaata cagataatca acagttagat aatgtacagt
    15661 ataaaaaatt tatattaatt tgtgtagata tagttatgtt attgactcat atttacttta
    15721 atgggcagtt caaacaaacg atagaactgt ataagcaagc atttaatgct actgttaaaa
    15781 cattgattga agattcaggt catttgctta ttgttcataa tggaatagtc attcataata
    15841 agttcttaat tttaaacgat tttggaaaca aaaatggtgt ttcaatatca gaagggtatc
    15901 aaccaacata taaaattcaa acggcttctt gattttatta aatcaaaaag ccataagtca
    15961 acaatc
//
LOCUS       DAFKMX010000092        15939 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFKMX010000092 DAFKMX010000000
VERSION     DAFKMX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.092"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..440)
                     /locus_tag="LZB08_002245"
     CDS             complement(<1..440)
                     /locus_tag="LZB08_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein complex assembly protein"
                     /protein_id="HBM7315743.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYT"
     gene            complement(571..1164)
                     /locus_tag="LZB08_002246"
     CDS             complement(571..1164)
                     /locus_tag="LZB08_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBM7315744.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(1229..2698)
                     /locus_tag="LZB08_002247"
     CDS             complement(1229..2698)
                     /locus_tag="LZB08_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBM7315745.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGRKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(2813..4204)
                     /locus_tag="LZB08_002248"
     CDS             complement(2813..4204)
                     /locus_tag="LZB08_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBM7315746.1"
                     /translation="MYFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLVQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKACKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISESGLNEY"
     gene            complement(4370..4618)
                     /locus_tag="LZB08_002249"
     CDS             complement(4370..4618)
                     /locus_tag="LZB08_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315747.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            4884..7013
                     /locus_tag="LZB08_002250"
     CDS             4884..7013
                     /locus_tag="LZB08_002250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBM7315748.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYDKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(7255..7854)
                     /locus_tag="LZB08_002251"
     CDS             complement(7255..7854)
                     /locus_tag="LZB08_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBM7315749.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(7872..10991)
                     /locus_tag="LZB08_002252"
     CDS             complement(7872..10991)
                     /locus_tag="LZB08_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBM7315750.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVLDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     NFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            11212..12036
                     /gene="pdaA"
                     /locus_tag="LZB08_002253"
     CDS             11212..12036
                     /gene="pdaA"
                     /locus_tag="LZB08_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBM7315751.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(12133..12918)
                     /locus_tag="LZB08_002254"
     CDS             complement(12133..12918)
                     /locus_tag="LZB08_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7315752.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            13383..13748
                     /locus_tag="LZB08_002255"
     CDS             13383..13748
                     /locus_tag="LZB08_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315753.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            13826..14380
                     /locus_tag="LZB08_002256"
     CDS             13826..14380
                     /locus_tag="LZB08_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBM7315754.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            14600..15274
                     /locus_tag="LZB08_002257"
     CDS             14600..15274
                     /locus_tag="LZB08_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBM7315755.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      15629..15725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            15779..>15939
                     /locus_tag="LZB08_002258"
     CDS             15779..>15939
                     /locus_tag="LZB08_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315756.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLV"
BASE COUNT         5411 a         2466 c         1751 g         6311 t
ORIGIN      
        1 gtataaaaac tctcaaatga tttagcaaaa tcacatttta ataattttaa aagacctcta
       61 agatttagta tatcaatgtc tttaggattt atctccagag ctttattaac catattaatg
      121 gcactagtta tataattttt atatgctaaa tccaatgcat tgttatatct tttatatgat
      181 tctaatttta actccttaga cgttgtgtct gactttgaat ttaaagctcc ttgcaagttt
      241 aatagagttt cttctttata atttgtagtc acataactct gttttaatgc ttttaatgtt
      301 tcatcaccgt cttcttcttc tatattaaaa aaagtcaaat cacttttatt atcttctatc
      361 aaagatatat ctaaaatttt tctaggcatg ctttctatgt cttgttttat atctttaact
      421 aaaataatac tcacctccac tctaaaatat atatttaaga taacatattt aatatgtcaa
      481 gtcaaaacta tacatatttt tccaccattc tcataataaa aaaggtaatc ctcttctttt
      541 cataaaaagg ttacctcttt ttcaatacta ttaactttta atgttgaatt tttttacttt
      601 tctatagagt gtagctatac ctatatttaa tgcctcagct acttttttca taccttctgt
      661 attacttcca tatttcttta tggcattttc tatagcttcc ctctcaaggt catctatagt
      721 cttaacttct tgtttataaa gttcatctag tttaaaagat attaaatcag acatactcct
      781 tacaaaagca gttaactcct tatcttttga aagtatcttt tctttttgtt cttcattaaa
      841 cgcagctata cccaagattc caagtatatg ctctcccatt ttaacgggag tacatatatc
      901 agctaactct ttacaatttt ctctatttgc acatttatta cagttctctt ctatttcttt
      961 atttataaca taggattttc catcttttaa aacaagagag tacactgact tctctgatat
     1021 cgtctctcct attttacctc tgaatattcc tgtacctgct atcctagtta aatcttttgt
     1081 aactaaagta acatctacat ccaagacatt agaaatattc tcgcaaatac tttgaacgta
     1141 attcgttata ttatttaaat tcaaagtaat ccctcctatt tagatttata ttttttatta
     1201 ctctacataa atcctattct tgagaacttt aagcttgcgg ttgtggttgt gtctcattag
     1261 gcttattatc agttggtggt gtttctggtt tttgttgatt gtttggattt tgattgtttg
     1321 tattttgatt attcttattt tcgtctgtat ttggtttttc ctctggtttt ggttccatag
     1381 tcccaactaa tataatacct tccttcttag gataatatga attagctatt ttttcagttt
     1441 taattttttt accatttgca tcattaaatg ttctatatgt tgatacagaa tatgcatttc
     1501 ttccactttt gtctactttt tctttacctt taggcattgt agggtcatta actcttttca
     1561 ttgtagtttg tgaaacgcca tctaattttg ttgatatcgt tatattttgt ttatcttctg
     1621 cacttccata tatattgcaa acaatttgat ttcctgaaac gtaatttttt atgtaaactg
     1681 gatgctttag attattttta aataagaaat ctattccact atctgcaaca gttgcatctc
     1741 ttccttttgg aacataactt gatggtatag tatgattttt tacattaaca atttctaaac
     1801 ctgcgtatag cacagaattg tataacgtag atgaaacttg acaaactcct ccaccataat
     1861 cttcttccat ctcaccttcc attattacag gtgcactttt atatccattt tccttacttc
     1921 ttttccctgt atgtttatta aatgagaaag tttctcctgg cataagtaat acattactta
     1981 tattatctgt tgctaatttt atgtttatac ttctatttat ttgagaagtt ttaaatgttg
     2041 ttgaatacgt acctaaattt gtatctatat tagcaagttg ctcttttttt acttttgctg
     2101 gagtagcttt tactactaac tcatttttat agttaccttt tttagtttga ttatcaaaat
     2161 tctccatact cttagaaata tccattttaa gaccatcaga atctggtata accttgactt
     2221 tttcaccact tatatcaagt gtagcatctt ttacatctac atctatttct ttagctatct
     2281 tttccatttc tgcttttaat ttatttttat cataatttat cacaactact aagttgcttt
     2341 ttctgccaaa atttgctgaa atagtttttg atagattgcc aaagaaacca ctctttctat
     2401 ttaaattata tgcgttttct actgttttat ccaggtcagt agataaattt aaatctttgc
     2461 tctttacttt ccaactttta tcattgtaat taaaatcaac attctctaat ttgtattttt
     2521 tagctaattc ctgttttgct tgcgatttag ttaattttcc aatatttact ccattaacat
     2581 atgtgttttt tgctattttc tcccctttaa attgcatgca cattatgcct ataaaaatta
     2641 ttactactaa tgctacagct ccaatagcta taactagctt tttgttcaca ttactcatcc
     2701 catacttctc cttacaataa aattatttat atatccctat tattatatta ctacataatg
     2761 cgccttaaaa ccccaaaaac tttttaatta tatattaatt taatattatt aattaatatt
     2821 catttagtcc actttctgat atggcatata ttttttcatt gtaaatatta atagcataaa
     2881 cttctttgcc tctaaataag cctttattta ctacagcata ataataaata tttttatatt
     2941 ttccaaacaa ttcttctaaa ctctttttac tttttacata tctatctgct ttctctatat
     3001 ctaatatctc aatcttttta tacttatttt ctttttcatc aagagtatta gttaacacaa
     3061 acttagcttt atcttttgta ataccaaatt gataacttcc atcttctaat attctaaagt
     3121 atccatcacc ataatctact ttgtaatctt ttttaccatc tttatatgaa tataaatctt
     3181 tagtttttgc acttaccaaa acttcattta ttaatttatc atactcatat acttcagcat
     3241 aatagtattt attatttttt atcatttgtg gaagtgagtt tatagataga tttgccttgt
     3301 ctttgccact ttttttagct attacattta taatgtcatc agatgttaaa gtatacaatg
     3361 ataaattctt tttatatttt ttttctaaac tctgtacctt ttcatcatct gaataatcgt
     3421 cttttaaata ctcaatatat tgtaatgctc tttcataatc tccagaagta tttgcctctt
     3481 cagctctact tatatagtaa tcatacattt cttcaataca ttgttctttc ccattttgag
     3541 ccatactgta atatttttca tcttctttta atactttatt atatttttca atagcttcat
     3601 aatacttaca tgctttttgg tcttttactg ctgagtcata aacatctcta gactctttga
     3661 ttgtttctat attctgtttg tatacatcta ggttactacc cattccattt aatattgaaa
     3721 cagtacttat gtagttaatg gcaatatcat aagtagtatt ttcttcttta tacatttctc
     3781 taactctatc agcttgaact aataagtcat ccacatctat acttattttg tttaattctt
     3841 tcacggtatt tattaatcca ataaaacttt cttgacttat gtctccatct aaatatttat
     3901 caccactatt taatagcaat ttatttattt ttttagaaac agacttatta aacctttcca
     3961 tttttgaatc agaaaacttt ttttctatac ttgtgtagta atcaacagct atagaatact
     4021 gtcttttctc tataatcttt aacaactcat tagcttcatt tcctacacta aactcgttac
     4081 tacttaagaa aaatactatt atcaaagcaa ttacacctaa tgttgataat gaaatcgcaa
     4141 ctatagattt ccttttttca tcaataaaat ctacagcctt ctcaaataaa tctctaaaat
     4201 acattatagt tccctctcca tttaataatc tttatatttt atggtaaact aaatttctta
     4261 ttggttatta tatcataatt cttgaaaaaa aataaaaaca caagttaaat cgtgttttta
     4321 ttttacctat caatcattat tttatacata aatatttcct ttacataaat tatgatatct
     4381 ttctttttat atccctagaa tgactctttt gttttttatt agtactgaca gttttcgttc
     4441 ttgtcttgtg tatagctttg ctatcaaata tttttctagc attgcataaa acaattccaa
     4501 ttgttaaaca aactgtaagc acacttccca tcaaaaagta ataatccaat cctatatgta
     4561 ctcccataac tgaatttatt aacactaatg caaaagtcat tgcaaaacat atcatcatgg
     4621 agataactaa aattcttaga atttttttca atccactcac ctcgtataat cactaaaatt
     4681 gacactaccc cttatccatt actataccaa attagtaaaa aaatagcaat tactattgtc
     4741 atttatatta ggaaaatatt aaatcttttt aataaatatc tttttttgac ataaggatta
     4801 cttaactttt aataaaatag taacactaat atgtattaat taatagaata aaataaatga
     4861 taatttattg aggggtgatt tacatgccaa atttgccaag tttagggtca aaggctcctg
     4921 attttaaagc caatacaaca aatggtccta ttagactctc tgactataag ggtaattgga
     4981 ttgttttatt ttcacatcct ggtgatttta caccagtttg tactacagaa tttttatgtt
     5041 ttgctaaata ttatgacgaa tttaaaaaaa gaaatacaga actaattggt ctaagtgttg
     5101 atagtaacag ttcacattta gcttggatgt ataatatttc tttacttaca ggtgtagaaa
     5161 ttccatttcc tattatagaa gatagagata tgagaattgc caagttatac ggcatgatat
     5221 caaaaccaat gagtgataca tcaactgttc gctctgtatt tattatagat aataatcaaa
     5281 ttctaagaac gattctttat tatccactaa ctacaggaag aaatattcca gaaatactta
     5341 gaatagtaga tgcacttcag actagtgata gagataatat agttactcct gcaaactggt
     5401 ttcctggaat gccagtaatt ttaccttatc ctaaaaacta taaggaatta aaaaatagag
     5461 ttaacagttg taataagaaa tattcatgta tggactggta cttatgtttt gtaccagata
     5521 attataatga tgaagaagtg agcaagaaaa ttgataatac ttgtagctgg aaaaaagaac
     5581 atactaaaaa cattgaaaat gaatgtaact gtgaacatga acatcatgac tacctgaaca
     5641 aagctcttga ttgtaaacaa gaacacaaga ctgatattaa agatgattgc aaccatgaga
     5701 aaaaacatac taaaaatact aacaaagttc acaactccaa acaagataag tttaaagata
     5761 agtcttgtga tgaaatgaat tttaactatg ataaagatga atcttgcgac aaaataaatt
     5821 ctagctatga taaagaagat agtagttatg aagatttcta taaacataat tataaaaact
     5881 acgattatac tagcgaaaaa aatactaaaa aaatagctat gaaaacttta aaagattcaa
     5941 aaaaattagt tagaccacaa ataacagacc catacaatcc aatagttgaa aatgcaaact
     6001 gtccagatat aaatccaatt gtagcagaat atgttcttgg aaatccaact aatgtagatg
     6061 ctcaactatt agatgcagtt atatttgctt ttgctgagat agaccagtct ggaaatttgt
     6121 ttattcctta tcctagattt ttaaatcaat tacttgctct taaaggtgaa aaacctagct
     6181 taaaagtaat tgtagctatt ggaggttggg gagctgaagg attctctgat gcagcattaa
     6241 cacctacatc tagatataat tttgcaagac aggtcaatca gatgataaat gaatatgctt
     6301 tagatggaat agatatagac tgggaatatc ctggaagtag tgcatctgga ataacatcaa
     6361 gacctcaaga tagagaaaac tttacactct tactaactgc cataagagat gttataggag
     6421 atgataaatg gcttagtgta gctggaacag gagatagagg atatataaat tcaagtgctg
     6481 aaatagataa aatagctcct ataatagatt attttaatct tatgagttat gattttacag
     6541 caggtgaaac aggtccaaat ggtagaaaac atcaagcaaa tctttttgat tcagacttat
     6601 ctttgccagg atatagtgtt gatgcaatgg taagaaatct tgagaatgct ggaatgcctt
     6661 ctgaaaaaat ccttcttggt ataccatttt atggaagatt aggtgctact ataacaagaa
     6721 cttatgatga gcttagaagg gattatataa ataaaaatgg atatgaatat agatttgata
     6781 atactgctca agttccatat ttagttaagg atggagattt tgcaatgtca tatgatgatg
     6841 ctttatcaat attcttaaaa actcaatatg ttcttagaaa ttgtctaggt ggtgtattct
     6901 catggacatc aacttatgac caagcaaata tactggctag aaccatgtct attggtataa
     6961 atgaccctga agtattaaaa gaagaacttg aaggtattta tgggcaattc taatagaatg
     7021 taggaaaatt ttatagttat ataaaaagca gattttatta tattaaataa gttgtatcta
     7081 attaatacta ttattaattg tttataaatt agatacttat tttttcataa tacaatctgc
     7141 ttttttaatc aaaatttatt taattataag ttgtttaatt acaagttgtt taattataaa
     7201 gtaggaattt agatatactc ttataccatg taattatgta taaatatttt tttattatac
     7261 accaatagct tttttagcta atttatcagc ttcctcatta tacttatttc cagaatgagc
     7321 tttaaccttt gtaaatttta cattaagctt attcttaata ctgtcataga aatttttata
     7381 tgctatagtt ccttctttat ttgttttcca aactccagta caccactttt caatcccttc
     7441 atagtcaaag tataaaatca agttttgaac attattgtca atacagtact gcattgcaat
     7501 tttagaagcc tctatctctc cagctacatt tctcatactt actaaagttt tctcttttcc
     7561 tttttcacta taagttttta taacttcatt attttttaaa atcacaacac ccgaaccata
     7621 catttttact gaatgttcat aacttccatc tacatatgct tctataaact ccttatcttc
     7681 ttgaaaactc aatttttctg acccatcaat atattcttta gcttcttgaa aagtagaaaa
     7741 actcttatat tcagctcctg agaatccatt tacttgtttt ttgcactcat cccatgtatt
     7801 atatacacct atatttttac cttttttcac agcataaaac ttttgttttg ccaaatatat
     7861 cactcccact cttaattttt atcttcttct ctcataaata tatctaaatt tataagatag
     7921 tttctatcaa aatattttac tttcatatct ttttttataa tatcaatatg gtacacttct
     7981 ccttttacag ctctttgtct tatattttca tttatatctt tttttataat atccttatca
     8041 acatcattgt tatccatttt agcatcaaca ttcaacgctt ttatagtatc aaatataaca
     8101 gattttagta ttaaacttct agatttattt aaatgtcgcc ttatataagt ataaaactca
     8161 ttgctattaa cctttgaaat tttacaatcg tgtaaatctt tctctaatct ctttaaaaca
     8221 cttatattag ttcctacatc aaaaatcaca aaacatccat agtcatattt gcttcttaaa
     8281 attctcccca tagcttgctt tacctttatg gtcatttgag gataatttat agtatagtag
     8341 tctataccat acttcttcat tatagtaaaa tacaaagggt cttttggatt tagattcgga
     8401 agcttgtcta aagttacaca aaccaaacca tcccctggaa tgtctacgcc ttcaaaacat
     8461 ccttttgagc caagtactac acattttcta tttaaatcat taaggtgtct aatgcccttt
     8521 ttatcagcat aaatttctaa gttaaaacta tgtaagtatt ttttcaatat ttcataagtt
     8581 ttttcttgtc tatctttact attaaaaaga gctagtgtat gaccttctgt aatctttgat
     8641 atattagata taattttact catctcattt ggaaattcag aatttctgta ctcacaaata
     8701 tcagaaaatc caactacaga gactctattt ttataatcaa aaattggttc tataatcttt
     8761 tccacattct taactctatc tattcctaat gtatttttaa aataagacat actttcagat
     8821 aaactcaaag ttgctgataa aaatattcct ttttctaatt gagataaaat attttcttca
     8881 aataaatctg ctatttttaa tggtactact cgaaattcaa agtcattata attcttatca
     8941 atttcaacta tccttgcata atcatccttt tcatcatact ctaaaaagat ttcaaatata
     9001 atctttatat cctctaaatc tcttgtcttt gcctttccaa atttatatat atctgcttct
     9061 ttatctatac tatcatcatc aatattccta taaattatta ttattattga aactaaattt
     9121 tttattattt tctcacaact caactttatc ttttcactat aggctctata acttatttca
     9181 gtatctatac catccttttt tagttttccc acaatttcat ctatttgcaa attcagttcc
     9241 catcttaaat tataattact tacattatta tattcattac aattaccaaa atttaatatt
     9301 gaatctatct cttcaataat gagatttatg cttctagcaa ttttttgttt atcttctctt
     9361 ccaattttta atacattata aaatttatca aaagccttta tttttctcat atttctacta
     9421 tattttttat atataaagct actattttga ataaactcgt aaggataaat ttcttgtaat
     9481 aagtacctta aagactttga atttataata cttgaaaaga aatcatatcc tttttcagtc
     9541 aaattatgtg cttcatctac tattatattt tctaaaggtt tttcatcctt atacggccat
     9601 ttagctaata gtgaatgatt tactactgtt atatgttctt cttttaattc ttctattctg
     9661 tttttatata aacaatcttt tttgcatttt ttaggtctgc acatatttgg gtcacaactt
     9721 acatttctta agtgagtact tatttccttg aagttgtcaa gcactaaata atttatttct
     9781 tctatatctc catacttgcc accttcaact aatcttttca aaaatattaa agataaaatt
     9841 tctcctttag tagggttttg agactcataa tcatctatat atgcttccag tctatctata
     9901 caaatatagt tattttttcc ctttatatat ccataactta cttttccatt tagacccaaa
     9961 gaatctaaca catttggaat atctttattt attaattgta tctgcaattc ctttgtgtca
    10021 gtagaaatta ataatctttt tttattaatt ctagcctcta atattgcagg caataaatat
    10081 cctacacttt tacctatacc agttggtgcc tctatacatg caattttttc ttcatcttcg
    10141 ctatttctaa acaactctct tattgtttta gtaagctcat attgaccagg tctatattca
    10201 tatgtaaatc cttctttatt ctcccaaata gttttgtatt ttaataattc ttcataattt
    10261 ttttcttctt catttaaaat ttttaaggtt tctctttcct tcttcttttc ttcttttgag
    10321 tcaaatatat tatattcttt ttttatattt atattatttg ataaatcata atttgcctct
    10381 tctaaaaatt tgctccattc ccaggttgga agattaaatt ttttaagata tgaatttatt
    10441 ttaaaactca atggctccaa agtagttttt tcatagttat taaattttac taaaaggcaa
    10501 tttacaatat ttattatatc aatagcatct gataaagctc tattttccac tttagaatta
    10561 caatttgtta aagtattttt taagtattct aagctataat ctttatgata tggttctaaa
    10621 ataactgcta attcaattaa atcaataaat ttgtttttta actttggcat atgatactca
    10681 aaaaactttt ttttcagact taaatcatga catataatga aactatcttc tacaaaattt
    10741 acaagtctat cttccaccag ttctatttca ttagctattt ctaaatcatt tttatctaaa
    10801 tttttacata ctgagaatac ttctacaggt acctcaaatt tgttttttat taatgattca
    10861 taggtatata ttttccagtc tttaaccttg acagctccaa cttctaaaat ttctgaattt
    10921 aaacaatcta atccactaac ttctatatct aaaaaaacaa catcattcaa tacacttatt
    10981 atattatcca tttttatatc ccttcaagtt aaaccagcct aacttattta caatcatttc
    11041 tctttatatt ttatcattta tatacacttt atttaagata attaatatca actttatatc
    11101 ttacagtaaa cttctttcat ttattatttg tatatgtgaa tatttttaac tataaatatt
    11161 caaactatat tataagaaat atactttaaa ttttaaataa gaggtgaaat tgtgaaaaaa
    11221 gatagcctaa aaaaatacat tatgatagga gcatttgctc taatactttt tggaattgct
    11281 agtataaatt ttaaatcatt agataaaaca aaaactcaaa tttctagtcc aacactcgac
    11341 acacatgaat atgattggta ttttaaccct agggaagatg gtaaacagcc atcacctata
    11401 aaagaagctg atttttttaa aaaatacgga gcttattatg taggaaatcc caatgaaaaa
    11461 gttatatatc tatcatttga tgctggatat gaaagtggaa acactccaaa attattagat
    11521 acgcttaaaa aacacaatgc taaagctcaa tttttcgtag ttgaaagtta tataaaaagc
    11581 aatccagagt taataaagcg tatggaaaaa gaaggacatt tagtttgtaa tcattcaaaa
    11641 agccatccat caatggcagg tattacagat tttgaaaaat tcaaagaaga aattacaagt
    11701 gtagaaaaag catacaaaga tgttactgga aaagaaatgc ctaagtattt tagacctcca
    11761 atgggtaaat ttagcgaaca atctttaaaa tatacacaag atttaggata taaatcaata
    11821 ttttggagtt ttgcttatgt agattggtat gaaaagaaac aacctactca tgaatttgcc
    11881 aaaaacaaaa tttattcaag aactcatcct ggtgcaatag ttttactgca tccaaattca
    11941 tcaacaaata ctgagatatt agatgaggtt cttacgcatt gggaaaaaga aggttataaa
    12001 cttaaaacac ttgattattt aaataataaa aaatagtatc ataaatttaa aaaaactcta
    12061 tcatgaatta tagtaaaaat atagttattt ataaatactt taattctcga tagagttttt
    12121 tattttttat agctatttaa aaagaattgt ccattcattt atctctttgt atcctatatt
    12181 atgatatatt ttacctgcta catcattatt ataaaaaaga catggaactt ttccttctaa
    12241 caacaattct ttactcaaat tgtaaaccat ataacttgca agtccttttc ccctatatct
    12301 tttatctgta cttacagaga ccaccattgc caaaaaacta gtttccatac cagttgacac
    12361 agtgctaatc atagtatcat catttctaat aaaatatgct cttacatttc cctcttttaa
    12421 atgttctttc ctttcttcta tgtaattttt acttacttta tagtctgacc tatttaaaag
    12481 ttttcctatt tcatcaatat ctttttcctg agcattttct attttatact ccttcatgtt
    12541 ggaaaaatct atttctttta tttcttttaa tacacaaaat ttattatcta gtttttcata
    12601 aaaatctttg tatttactta ttagtctatc tattacacat ttttttccac ttatgctttc
    12661 tacatctaaa tcttttatat gattcttcat ttctcctata ttaaaattat ttttaaaact
    12721 ataaattata agtgttgact tattttttaa tattatagag gttatatttc catcttctac
    12781 ctgagaccat atatcttgat attgagagct aaatccataa ttctcaacgt atccaattat
    12841 gaataaattt agactttctt catgtgatac atattgccaa aattcttttt ctaaagtatt
    12901 atctattttt acaatcaagc agatatcctc cttctatgtt aataatgata taccttataa
    12961 tttatagtat catttaaaat cattatattc taccaaatat tctaaatttt taaatatcta
    13021 tgcaatatgt tcgtatttat ttttatattc tgttactgct tcactccaag cttcttctac
    13081 tgaaatcccc ctcttatttg caattttttg tgctaattct attattttca tagcttttct
    13141 tgatatattc atatatattc ctcctctaaa ttatcataag ttattttaaa gttaaatttc
    13201 tatagtcatg ttatatattt atttacttat atcttactaa ttatttatgt actaaatatg
    13261 aactacaata gaagaaattt agaacttaat aaaacataaa atttatatac agttcatata
    13321 tatttgtcat atcacaagac aagtgatata atttattatc attttactgg gggtgatatg
    13381 ctatgaaaat aagagctgaa tatacatgtc ctcttgaaat tgtacatgac ataatcaaag
    13441 gaaaatggaa aactatcctt atatttgaat taagacaatc taataaaaca ttttctgaat
    13501 tgcaacataa tatttatgga ataaatcaaa aaatgttgat tcaacaatta aaagagttac
    13561 gtgattttgg gattattgat aaaataagtt ctgctggata tccattacat gttgaatatt
    13621 ttcttactaa tcgtggtaaa aaaatgttaa aagcagtgga aataatgcaa gaaataggaa
    13681 tcgaatatat gcttgaacat ggtcaacaag aaattctaga taataaagga atttgctaca
    13741 ataagtaatc ctatacatac caaaaagtaa gtaatttaca ttttttacaa tagtattata
    13801 ataaaaataa ataggaggta taatcatgtc tatagcttta atattgattg atattcaaaa
    13861 tgattatttt aaaaatggaa aatgtgaatt atttcaatca gaagaaactg ctgaaaatgc
    13921 taaaaaaata ctactgtttt ttagaaaaaa caatttacca gttattcata tacagcatat
    13981 atcaacagat aaaacagctt ctttttttct tcctaatact catggttcag aaataaataa
    14041 aattgttttt ccactagata atgaagaaat tataattaaa cacacaccag atagtttctt
    14101 tgaaacagat ttgcaaagta tccttgagaa aaaaaatatt actaatctag taatctgtgg
    14161 aatgatgagc catatgtgta ttgatacaag tgtaagaaca gcaaagaaac ttcgatacga
    14221 cataactcta ataagcgatg catgtacaac gaagaattta tcttggaata atatagaaat
    14281 taatgcaaat actgtacatc aagtattcat ggcatcacta cagaacagtt ttgcagatgt
    14341 aaaaaacaca gataaatttt tatctaacta tcacaaataa aaacatacac tatgttaaaa
    14401 gagcaagttt gatttattta agcttgctct ttaatcattt aaaattagat gctttctatc
    14461 atttttatat acataatata cgtttataca ttttatattc atatataact attatataaa
    14521 ttttaaactt tactatattc acataaaaaa tgtagtatta tagtatatat aatattatat
    14581 agaagggggt gctagataaa tgaaccatca tatattgatt caatttgaat atatagcaag
    14641 aatcattact gctggtttct gtggggcttt aattggatat gagagaaaga atagactaaa
    14701 agaagctgga atacgcactc attttatagt ggcattagct tcagcactta taatcattgt
    14761 ttccaagtat ggatttaatg atatagtggg tactccaggt attggtctag acccagcccg
    14821 cgttgcagca caagttgtca gtggtatagg ttttttaggt gctggattaa tatttattag
    14881 aaatcaatca gtaagtggtc ttactacagc tgctggtata tgggcaacag ccggtacagg
    14941 tcttgccatt ggctctggat tatacttagt tggtattgtt tcaagtatca ttattgttat
    15001 tgtacagata acacttcata gagataggaa atggatgaaa ctccctacag cagagcaagt
    15061 ttctcttaaa atttctaaca gcactgattc catagaatat attgaaggta aatttaaaaa
    15121 atctaatatt gaaattataa acatgaatat taaacgcctt gatggggatt ggttagaagt
    15181 agttatatat actaagttac ctaaaggata taaaaaaaca aatttattaa aattatttag
    15241 cgataatcct cttatagaaa ctttagaaat ataatataaa aattgaagct taaaaataat
    15301 aatatattta atcaaggtca atatataaat aattaaggga tgagtctata taaaattcat
    15361 cccttgtttt ttgccattca tatatatttt ttcaagatat ttttgtttat ctattatcta
    15421 gccaagtaac ccttgcgaac taaacttttt aatacaaaaa cttcaataca aaactaaata
    15481 taatttttat taaacattct atacatatag tttgtttctt gagtgaaaaa tagatttcat
    15541 attgttaaga tattgttaaa tatttcaatt ttctgacaag tatgttgaac aataacttat
    15601 cctagtgtat tattttagtt gtagaataaa actaaatcgg caaaactaga gaaatttagt
    15661 gacgcaaagc tatagggact aacgcttata aaataaacta tgagttatgt cagccagttg
    15721 ccaaaaagat attgttcttt ttgttttatg aaagtttttt agggggatag taatatatat
    15781 gtttagagat gaaatggata aatgtacaca catgttaact gcttatatta gtagtttata
    15841 tgattattgt gattttatag atacacagct agatgatttt atactagagt acggagaaaa
    15901 tgtagtagaa tcttgtttac atcaagtgat ggtattggt
//
LOCUS       DAFKMX010000093        15915 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFKMX010000093 DAFKMX010000000
VERSION     DAFKMX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.093"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..627)
                     /locus_tag="LZB08_002259"
     CDS             complement(<1..627)
                     /locus_tag="LZB08_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315757.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLI"
     gene            complement(1037..1213)
                     /locus_tag="LZB08_002260"
     CDS             complement(1037..1213)
                     /locus_tag="LZB08_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315758.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1267..1363)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2159..2560
                     /locus_tag="LZB08_002261"
     CDS             2159..2560
                     /locus_tag="LZB08_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBM7315759.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            2557..3624
                     /gene="rsgA"
                     /locus_tag="LZB08_002262"
     CDS             2557..3624
                     /gene="rsgA"
                     /locus_tag="LZB08_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBM7315760.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVYRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            4160..4858
                     /locus_tag="LZB08_002263"
     CDS             4160..4858
                     /locus_tag="LZB08_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315761.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            5036..5926
                     /locus_tag="LZB08_002264"
     CDS             5036..5926
                     /locus_tag="LZB08_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315762.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLEKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGVSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(6326..7282)
                     /locus_tag="LZB08_002265"
     CDS             complement(6326..7282)
                     /locus_tag="LZB08_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7315763.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7985..8980
                     /locus_tag="LZB08_002266"
     CDS             7985..8980
                     /locus_tag="LZB08_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7315764.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGTGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8958..9821
                     /locus_tag="LZB08_002267"
     CDS             8958..9821
                     /locus_tag="LZB08_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBM7315765.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            9818..10612
                     /locus_tag="LZB08_002268"
     CDS             9818..10612
                     /locus_tag="LZB08_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBM7315766.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            10897..12276
                     /locus_tag="LZB08_002269"
     CDS             10897..12276
                     /locus_tag="LZB08_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7315767.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            12724..13437
                     /locus_tag="LZB08_002270"
     CDS             12724..13437
                     /locus_tag="LZB08_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315768.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(13709..15247)
                     /locus_tag="LZB08_002271"
     CDS             complement(13709..15247)
                     /locus_tag="LZB08_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBM7315769.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         5763 a         1854 c         2392 g         5906 t
ORIGIN      
        1 tattaaactg tataattttt gttcatcttt ttttataatt aaaactacgt agttctcata
       61 atttctctta tatatatact tatcatcttt tttaacttct aacttatatt ttttaaaatc
      121 ttcttcaatt ttatttttat tacttttatt aatgttaaca gaatctcttt taaatttatc
      181 aatatatttt tctatttcat taatattatc tttgcttaca actataccac cttttaaatt
      241 aaaaactttt ttgaaatttt tttcagaaaa tccttgaata aaataacttt gtccatctcc
      301 attaatacta tcttgagtat caaatacttc aatttctttg ttaggtattg gtatatcaaa
      361 attccaagat tcctttagca ttgaaatatt agctgttttt cctaaaaaaa gattatatag
      421 gatacttcct aataacaaaa cacctattaa tagtagtgtg attaatacaa tttttctctt
      481 tttcattttt cgttcacaac cttttgaaaa aacatgattt aattcatcat ttgaaggcat
      541 gtcttcaaga ataatttttg ttgtgatagg aatgctttct tgaaacatat catttgtaat
      601 atcaaaatct tttctttgat tactcaatat ccatttcttc taatatttct tttgattttt
      661 tctagtacat tgaatactct tatatacatt ctcctctttt aaaattaaaa gttataaaag
      721 aataagttat ataaaaaatt attatatttt ttaatatagc ttatttgcaa atttataatt
      781 ttgttaatct aatccttcaa actaaaaaat catttatatt tacagaattt cattttaaga
      841 gatgtttatt ttcacataga taagagaatt taagttctca tatttattat ttgatacaaa
      901 tttacatctt tattatatgt attttaaata aaaaataaaa ttatgaatac tagaaatcta
      961 tcttaattag ctttctaaca taaggctatt taaagtagat aaaactaaaa agggttctct
     1021 agaaaaccaa atctatttaa ttatacttac ttactaatac catcacttga tgcaaacaag
     1081 attctaccac attttctccg tactctagta taaaatcatc tagttgtgta tctataaaat
     1141 cacaataatc atatgaacta ccaatataag cagtcaacat atgtgtacat ctatccattt
     1201 tatctctaaa catatatatt attatccccc taaaaaactt tcataaaaac aaaaagaata
     1261 tctctttttg gcaactggct gacataactc atagtttatt ttataagcgt tagtccctat
     1321 agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta caattacaat
     1381 aatacaatag aattgcttat tattcaatat acttatttga aaattaaata aatttacaaa
     1441 tatgaacgtt attttatatc aaaaaaatag actatgcatg tagaatatat ggaaaaactt
     1501 gattgtaggt tttgtatgaa aagacagttg ttattaattt aagtgagata aattgaacaa
     1561 taggcataaa atgagaaaac aaataatagc agtcaatttt tattattaat actaggtgaa
     1621 cattaaattt taaaatacca ctagatttta tagaataaga aaatgagaca aacataaata
     1681 tatgaatata ttaaattata cttgaacata cttatatttt atgataaaat ttcaataata
     1741 attattagtg aaggagtgtc ttgcctgatg tcagagttat atataatggt aaccaacaaa
     1801 taaatagttg gagacgcata tgggttctgt ttaatcagaa tttttaagtg tatttttgat
     1861 acatttgatt gttgtgtctt actctgcctt tatagataac taacattaga caacaaataa
     1921 taatattaca tattactttg ttaaattgtg gtgtagtggg ctttaatctg ctacactttt
     1981 attaatttta cacagtatta ttttgaatat aattatgttg aaatagaaga tattaaattt
     2041 tctagtccac aggggttttc tgtttttgtg taggtaaata tgtataccac ataaaaaggc
     2101 agatataaat acctgtggac tttttttgta gaaaatactc gatatggagg aaataaaaat
     2161 gaattataaa aatagaaaac gagcttatta tcaggaaaat tcctgtatgg ataactttat
     2221 ttgcaaaaat tgtggttgcc ttgtagttcc tgaaggagct ggtacacaac atagaaatca
     2281 ttgtccacat tgtttacata gtcttcatgt agatatcatt cctggtgacc gtgctgcaga
     2341 ttgtgatgct aatatggaac ctattggagt gtgggtgaga aaaaatggtg agtgggcaat
     2401 tattcatcgt tgtacacgct gtggacatct tagttctaat cgtattgcag ctgatgataa
     2461 ttcagtgaaa ttaatgtcaa ttgcattaaa accactggca cagccccctt tcccactaga
     2521 aagaatcatt gaaaagttgg aaaaggagga aacagaatga atggtatgaa acaaatcaat
     2581 ttacaagact atggtctaaa taggaatttt attactcaaa gtatggaata tcatgattta
     2641 taccttgctc gaattatctc gcaggaaaag aacttttatc aagctgtatg tgtaaatggt
     2701 gtactgtttg cagaggtttc aggaaagctg cgttttgagg ctactataca atctgatttt
     2761 cccacagttg gtgatttcgt tatgttggat cgttgcaata ataaaaatgg taatgctatt
     2821 attcatcatg tgcttcatag aaaaagcttg tttgtgcgta aggctgctgg aaaggtaaat
     2881 gaggagcagg tggttgcagc taatattgac attgtttttt tgtgcatgtc actgaataag
     2941 gattttaaca ttcatagatt ggagaggtat ttgtctttaa catgggaaag tggagctatc
     3001 ccagtagtag tacttactaa atcagactta tgtgacaatg tgtatcgtaa attacaagta
     3061 gttaattcca tagccttggg tgtagatgta gttgttacct catctataga gccagatggt
     3121 tataaacaga tttctaagta tattaaacca ggctgtactg ttgccttact tggttcttca
     3181 ggagttggaa aatcttcatt aattaattgt ctgatgggtg gagaattttt aaatacaaag
     3241 gaaattcgca tagatgatga taaaggaaga catgccacga ctagacgaga gctattttta
     3301 atttcaggtg gtggtattgt tattgacaca ccaggtatga gagagttggg aatgtgggat
     3361 gtttcagaag gtttagataa aagttttgca gatatagaag cttattttgg aaaatgtcat
     3421 tttcgtaatt gtactcacac ctcagaacca ggctgtgcta tctgggaagc tattgctcat
     3481 caagaacttt cgcctaaaag atgggagtcc tatcgtaggc ttaaaactga agcagtattt
     3541 gcagacaata aaagcgaata cttagcaaaa aaagaaaaga aatttaaaaa tatctcaaaa
     3601 ataaataaga agattagaag ataagtgtag tttttttgtt cattatatga gtctatatac
     3661 tattaatata ttaaatttgc catcaatttg ttactttaat gtgattttga tggcaaattt
     3721 ggtttgtggt attatttaaa gcaatttatt aattttttta ataaagtttg attttttata
     3781 tgatgatata tacatgtgat aatgtataca tatttgttac gataggctcc tttataaaga
     3841 ggtcttatgt aagataagaa aagtcatatt tatactaata cataatagag tatatgctta
     3901 tgtatgatag agatgtttct taagagcaaa actgcaatat tgctaattta taaagtaaaa
     3961 atatatgaat tataaaatac aaataatata tttataaaaa ttattgtttt aatttataat
     4021 aaaaattttt tgttgaaaat aatgctcgaa cctataaaac tattaactaa tatttgtaaa
     4081 tgtatacttt tgatgataaa atataattaa gctaagttgg tttttataaa cataaattta
     4141 ttttagagag gtgaaataaa tgaaaatatc tagtcaatat agaagtcaat attcatttag
     4201 atatgaaagt aatataaata atacaagaat aaatgaaagt atggttaaga aaaatgaaac
     4261 tgtaggaaaa gacacttatt tatctaatat tatgaaacaa aagcaagaac ttaatgatag
     4321 aattagagat ttaaaatata gacaagaggt ttatactaaa aaaataaatg acgcaattaa
     4381 gaacttatgt aaatcagaaa taagagaaac aactaataac ttttctaata tagaaatagg
     4441 tattaaaaat agcattatag aagataaaaa taaaagtaca atgttagatg aaaattcaac
     4501 ttatctaaat acaaatgatg aaaaagaatc tttaatcact aaagagtcta atgaaaaaat
     4561 tgaagaagaa atattaaatg atgaaaaatt agaagagtta gaacaaaaaa aggattataa
     4621 agaggattct aataaaaaag agaaagtatc tgaggactta tctttagtag gtaaaactcg
     4681 tgaagagctt gaaaatatgc ttaaaaattt tataaattta acacaagaag aaataatgaa
     4741 acttgagtcg agaatagaaa agttagataa agatgctgaa gaatacaaac aaaattcaaa
     4801 gactaatata tttgataaaa cagatgaaca aaaaaaacat ataaatgtac tgatttaaac
     4861 aatatgtttt aaaaaaataa gtttattata taatgaaaat ataaaggtac aatttcaata
     4921 cacttctatt aaaccaataa tttatgaatc agacttaatt tcaatttact aataaccata
     4981 tatttatgag ttaataattt tatgttttaa aaagaattaa ataaggggga gtagtatgaa
     5041 gaagaatata ttaactacta tgattaccat gattattata ttgatgatga gtatgacagt
     5101 aatggcagag ccatatcaag aagatgaaac atctagcttg gaaaaacaga gtgcaaatga
     5161 acgtaatcag actaatatag gcacaatgga tggcagagtg caaacaaatg caataagtaa
     5221 aggtgctaat tatgacccta aatatcctct taaaggaatg ttaacacaac ttggtcttaa
     5281 tacaacaggt aaagagggat gggatttact ctatggagga aatcccaatg gagttgagct
     5341 tcgagcagat ggtaaatatt attatataaa taagggtatg gaagggctag gattttatgc
     5401 ggcagcattg tcaggtgtct catatcagag aataggcttt ggaaaacttg accctataga
     5461 agtaatagct gatggaaatt ggatttctta caaacaagct caagatacac ttacagtaat
     5521 caggaatttt ttaaatagtt ttgactggag aaatgcatct gagatggaaa gagctaatag
     5581 agctgcaaaa ttggtaaccg aagcaaaata tgcagactct aaatattgta acatagtcta
     5641 tggtaatctg gttgataaac gtggtgtatg tggaagtttt gcaagtagct ttcatttatt
     5701 aacaaggtta atgggaatgg attcactatc tatattaaat ccatcattaa atcatgcttg
     5761 gaactatata caaattgatg ggaaatggta taggtttgat gggtctgaaa tatcagcttt
     5821 tggtggatca ttagactttg attaccgtaa attaaaggat gcaacaagag agatgactac
     5881 ttactatgat gctaaggcat tgtctatatt aggatttaat caataaaaat agggaaggct
     5941 tcttatggag ccttttttat tgatttaaaa tttaataagg aattttatat gaaaatttaa
     6001 tcaataaaag caaaaaaggc tctttataaa gcctttctaa atgttttttt aagtaaaatt
     6061 ttttatatca acatcagttt taatcaaaaa atataacatt ttggatttac ttttaaaaat
     6121 aaaattatta aaatgtaata ttaatcatgt taataatgtt tttagtttat tatattctat
     6181 aatagtggtc atgttgacta ttgtagtttt attaaaaata gttggtttat ataaatatat
     6241 gcaaagatag cttgttgttt actttaccta ttagtatata ggacaagata ctctaataaa
     6301 tttgcctatt ttaatatctt ttacactaaa accaactaaa gtttccttta ttatttcgtt
     6361 ttgaataata caaagcataa tccgattgtt tgtataaaga ctggaaagta tcagagttat
     6421 tttctaattt agatatacct atagaacaag aattactctc taggcataat tctaaaactt
     6481 tctttgcttt attttcaaca ttctcttttg attgattact atattcaaaa acagcaaatt
     6541 catctccacc aagtcttcca ataatagcat catttacgaa tactttagtc aaaatatttg
     6601 aaaattgtat tagtatttca tctcctcgaa catgaccaaa gatatcattt acctgtttaa
     6661 aattatctaa atcaaagatg taaaagtatc catgtgaaac agttttaagc atttctttta
     6721 tgtgtacttc aaaaaaatgt ctattgtata gtccagtcat aaggtctttt tctgatttta
     6781 gtttaaattc atttgccatt gctagtgctt cttgtgcttt ttcagttatt gttttagggt
     6841 aatactcatc tttcatttgg tcaaagtttg gaatagaatg gtgaacatgg ataagtttaa
     6901 tcatatcatc tgtaactctg tataccatag taaatcgagt atccatttct acatagatat
     6961 ctttttctag gtaatctttt tctattaccc aaagagtacc atatactata aaagcttcgt
     7021 ctgtgacttt ggtacatgaa taccactcat ctataatctc aaagtctatt tgctctgatt
     7081 ctgtttggtc tttttcaaga cctataataa attcttctaa tgtttcgtaa aattcatgtt
     7141 ttcctgttcc aattaatgca aggttgtcat ctaataagga aataataaat ttaaggtttt
     7201 cttcttctgg atttaaaata taaatatgcc aaattttttt tgataaatct cttaattgtt
     7261 tttgtattgt attattttcc atagattacc cccttaagat ttacagttta tattttaatg
     7321 tattataagt tgaactctta ttacaatatt attataatat agaactaaag aaattacatc
     7381 ctgtataggt aaaagtatat cttttacaat aaaaatatta cagtaaaatg accagtttta
     7441 atatacgtat tatattttta atttataaac gaatattata aatgaaattt aaattcaacc
     7501 ttgaagaaat aagctaaatg tattagtata taaatccatg cttgaacata aataaccttt
     7561 atgatatgat atcaataata atattatgag aggagtgcct tgatgtcgga gttatataat
     7621 ggtaaccaac aattaaatag ttggagacac atatattctg tataatcaga aatttcaagt
     7681 gtatttttaa tacatttaat ttttgtgtct tattctgcct ttatagataa ctgacatatg
     7741 acaacaaata gtatttaaaa tatatattac tttgttaaat tatggtgtag taggctttaa
     7801 tctgctacac ttttattaat tttgtatagt attattttga taactatttt gaaatagaga
     7861 gcattaattt ttctagtcca caggagtttt ctgtttttgt gctgttaaat ataagcacag
     7921 aaaaaggcag atatatacct gtggactttt tttatataaa ttatgaaata tggaggtagt
     7981 taaaatgagt ataattgaac ttaatgaact gactaaatct tatagaagat ttgagaaaaa
     8041 ggcaggattt atggggagtg taaagtcttt atttaatagg aaatatacag aaaaaacagc
     8101 tatatcgtca tttgatttaa agatagaaga aggagaattt gttggattaa taggtcaaaa
     8161 tggaactggc aaaactactc ttattaagat gttaactggt attattgcac cgtcttctgg
     8221 agaaatatct gtaatgggtt attaccctaa caaattagaa aataatttta aaaaacaata
     8281 tgctgttgtt atgggccaaa aaagtcaact attctttgaa ttgacaccag cagatacgtt
     8341 tcttttattt aaagaattat atgatatatc taatgaagaa tataaaaaaa acttggatta
     8401 ctttatcgaa ttatttgagg tatctgacta tttaaatgtt caagttagga cgctttcttt
     8461 aggagaaaga atgaaaatgg aactaatagt agcccttcta cataatccaa aaatattatt
     8521 tttggatgaa ccaactatag gcttagatgc tatagcccaa aaacaaatta ggtatttcct
     8581 aaaagaagta aatgaaacaa aaggaactac tattatactt acttctcatt acatggaaga
     8641 tattaagagt ctctgtagta gaagtattgt tgtagaaaat agccgtaaaa tatatgatgg
     8701 ttctacagag aaactattta atcattttca aaaatataaa aaaataacag ttagtttctc
     8761 caatgaaact aacattgtat ttccaaaaga ttgtaaagtt gttgaacaaa caaatcataa
     8821 gatatcagta attgtgccaa aagaaagagg aaatgaaacg attaaatgga tttttgataa
     8881 atatgatgtt aaagatgtaa tgattgaaga agaagatata ggtagtattg tagagagaat
     8941 atataagaaa ggaagtgatg agtattatga gcaactataa tgaagcaagt tcaaattatt
     9001 caatgtttta taaatttaaa cagagtatta gaaggtatta tgaagtagcc aaaattaatt
     9061 ttaaagtgca acttatctgg agatttgatg tggtggttaa tatggtgttt acgattgtaa
     9121 aaattttatt tgcttatatt ttatggaaag caatttttgg tgaaaataaa atagtctctg
     9181 gttttacatt taactctatg ttgtcttact atataataag ctcattttta gcaggtattg
     9241 atatgtcaaa gagtaccagt gaagagatat gtaatcgtgt aaaattgggc accttttcaa
     9301 agtacatgat tattccaatt aatgttcaaa attattttat tgctaaaaat tttggaatga
     9361 catgttttta ttttgtattt aatatgattt ctgcatttgt atggattttt atatttaaaa
     9421 tagattttgc ctttacatca aatccagttc aaatattaat ttcaattttg atgatttttc
     9481 tagggtttat ttttatgatg aaactccact tcttcattgg gattttgtca tttaaatttc
     9541 ttgagattag tttttttaga atgataattg aaaatattgt tgaatttatt acaggaacaa
     9601 tgataccact tgtactctta ccagaaacta tagtaacagt tatgaaggta tttccatttt
     9661 attatacaac atatttgcca tctatgttac taataggtag aaacagcaat gaggcaatac
     9721 aaggattaat tattctagca ttatgggttt tatttttttc tgtagtaaat aaagttatgt
     9781 ataaaagaat gagaacatta tttgatgggg tgggtatatg atgaaaaaaa atttgaaaat
     9841 aatatttatg ttattaaaaa tgaaattatc aaagatgatg gtatttagat tcgatttttt
     9901 tggagcattt tttgtagata gttcattatt tatattacaa cttctaatgt ttaatagtat
     9961 atattctcat gtagactcaa tagggggatg gcaacaagga gagatgttaa tatttatagg
    10021 aactttctca ttaataaatg cgataaatat gacgattttc ttttttggta tttatgatat
    10081 tccacgaaaa atccaagaag gagaattaga ctactatatt acaaaaccat tagacccact
    10141 atttaggtta acatttgaaa atattaatat tggttcatca ccattaattc tagcaagtat
    10201 acttattgtt ttatatggaa tatctgagtt aaactcagag ataactttag ttatgataat
    10261 aatatatatt ttcatggtgt tattgatgac attacttttt tatgatgtat gtatcatact
    10321 tagaacagtt tcattttttg ttatatcatc ttctgcgata atgagattag aagataattt
    10381 attgccaatg aatatgaaaa ttcctggtgt aatatatgaa ggtgttttta aagtattatt
    10441 ctatttaatc ttaccatatg gaattatgtc gacaattccg actcaaattt tggctgggac
    10501 aataacaatt aatggattaa tatactcaat tttaattgta tttttgttta cattgtttat
    10561 gtggagattt tggaagttag gtatgagaca ttataaaagt gcaagtagtt aataaattag
    10621 taaaaataaa caatgtataa ctatatataa atacgtttca tatgacacta aactatgtga
    10681 acaagtattt gcgctaaagg cactgaaagc tataaaatta ttaaagtaag gatttaaaag
    10741 tttctttagt agaaatataa ttgtgtaaaa aagcaagatt aattaaaact atatccaaat
    10801 aattagatat aattcttatg cagaaaggag gtctgtaaat gtctaattgt ttggaatata
    10861 ttttaaaaat aattaatgta cagaaaaata aacaaaatga gtgatgtata ttattcagaa
    10921 aatcatgaac aagataatct ttatgagcaa gataatttac tggcacaaag atttaattta
    10981 tttactctat tgaagtttgc atttcctact atttttatga tgatttttat ggggttgtat
    11041 acaatcgtag atactatatt tgtatcaaga tttattaata caaatgctct atcagcaatt
    11101 aatattgtat gtccaattat taatcttata gttggaattg gaactatgct tgccactgga
    11161 ggaagtgcta ttgtagcacg taagatggga aatggtgata ttgagggagc aaataaagct
    11221 ttttctttaa tcgtattatc aggagttgta attggtgtaa taattactgg tataggtatt
    11281 gtatttatgg attatatcat tatatggtta ggagcaagtg aagttttaat taaatattgt
    11341 cgttcatatc tttttattat cttggttttt gcttctgcaa atatactaca agtattattt
    11401 caatgtttat ttgtaacagc tggaaagcct caatttggat tatggctagt gttaggtgct
    11461 gggtgtgcta atataattct tgactatgtg ttcattgttc tgttacaaat gggtattagt
    11521 ggagcagcac ttgcaactgg agtaggatac ctaattccta ccgtttcagg tatggttttc
    11581 ttttcaaaaa ataaagggtt acttaagttt tgtataccta gactagataa aagaatatta
    11641 tttgaagctt gttttaatgg ttcatctgaa atggtgggtc aactatcaac agcagttact
    11701 acttttttat ttaatgctat gatgatgaaa ttaattggtg aagatggggt tgcagctatt
    11761 acaataatta tttattctca atttctttta acttcgcttt atataggttt ctcaatgggt
    11821 gttgcaccaa taataagtta taactatgga agtgaaaata atacgcagtt aaatcatgtt
    11881 tttaagattt gtatattatt tatttcagta gcatctttat tggtatttat cttttctata
    11941 tcatgttcac caatcattgt agagattttt tctcaaagag gaagccgagt ttatgaaatt
    12001 gcaaggtctg gattttatat attttctttt agttttattt tttgtgggta taatattttt
    12061 acttcgtcta tgtttacagc attatcaaat ggaaaggtgt ctgcaatatt atcttttcta
    12121 cgtacatttg gatttataac tataggttta ttgatattgc caaatatatt aaatataaat
    12181 gggatatggt tagcagtacc aagtgctgag attttaactt tatttttaac cattttttgt
    12241 atatgtaagt ataagaatag atataaatat atgtagatat taaataaata gtttaggtag
    12301 atattttatg ttttaataag taattcaatt agtgggagat atatactatt gaaaattaaa
    12361 gttataattg atataaagaa ttaataaaaa taacatataa aagtttaaaa gggagttgaa
    12421 cttaaagttt gattagaaaa agtcaatttt aagacaactc cttttatttt actattgtat
    12481 taatctgtat ctgtaagtgt attagtctaa tattataaac tttggatgaa attaattttc
    12541 tataggtttt aatttatcat acactttatt ttaaagaaac ttatcttaat tttcatagtg
    12601 attgaatttg tctattagag atgttactat tagtaatgta aaagcaagag tagtgaatat
    12661 aaaaaacaag atagtgatac tgcaagatta gaatataata aatttttatg gagagatttc
    12721 aatatggata catgttttaa taaaaaaata ttacttgttg atgatgaaaa ggatatagta
    12781 gatttaattg aagaagtact cataaatgat ggttttaaaa acattattaa agcttataat
    12841 ggtctagatg ctatttcgct gtgtaagata gcatgtccag atgttgtcat tcttgatata
    12901 atgttaccag atatagatgg tatagaagtg tgtaaaaaaa ttcgagagtt ttcatactgt
    12961 tctattcttt ttttatcatc aaaaaatgat gatattgaca aaatactagg tcttagtagt
    13021 gggggagatg actatataac aaagccattc agtccaagag aaattgcttt tcgagttaaa
    13081 gcgcaattac gtagacaaca gtatcaaagc attgtaccat cagatagtga agttattaaa
    13141 ataggagata ttactattga tatagaagga aaccgtgtat ataaagatag aaatgagatt
    13201 gagttaactg gaagggagta tcatctttta agctacatgg ctcaaaatgt aaataaaatt
    13261 ataggaaaag aaagactcta tgaacaagta tggggagtat atagtagtat ttgtgacaat
    13321 acaattatgg tgcatatacg acatataaga gaaaaaattg aagataaccc ctcaaatcct
    13381 aaaatactta taacagttaa aggactagga tataaacttg tgaatagaat cgattagaat
    13441 agattttaat attaatgaag atatctcaag ttgaattttt agttaatgag gcattctttt
    13501 atgggtgaaa tcaaatgtat tttcgttatc tcaacaatga agaagaggtc tatctctatt
    13561 ttgagatagc ctcattaagt ttatatcctg gtaatgtact ttatgttcta ataaaatttt
    13621 tttagtttat ttgcagtgaa acattaacta gaacattaaa atttgcattg aaataccaat
    13681 taaataatat tgtatattgt gtataaactt atactaattg tttttctata ttaaatactt
    13741 tatcaatcca acctttataa aatttttctt catgggaaac taggattata cttcctctaa
    13801 actgtttcaa agcattttgt aaagcatcct ttgtctctgc atcaaagtga ttggttggtt
    13861 catccagaat aagaaaatta caaggagata acatcataca acataattta accttagatt
    13921 gttcaccacc acttaaagta gatacactcc ttgaaacatg ctcttccttc acaccacatc
    13981 tggctagatg acgtctaatc tcttttgtat ttaattttgg atatttatct gctactattt
    14041 gaagtggggt tttatcagga ttttcccatt ttaaatcttg ctcataataa ccaattttaa
    14101 cttgttctga aaactggaaa tctccagata tacgaggaat atcttttact aatgtcttta
    14161 acaaggtaga tttaccgatt ccattaaaac cagttataac tattttttga cctccatcaa
    14221 cagaaaaatt taattttgga agtaaagaat agtagtatcc aacttcaaga tttgttatag
    14281 taagagcatt ttgagccgat atttcaattt ctgaaaattg aatatttggt tttcctgtaa
    14341 aactaggagg agctattctt tctattttat ccaattgttt ttgtcttcct cttgcaattt
    14401 ttgaatttac tccagcttta tttttacgta taaatgtttc ttctttttct attttctttt
    14461 gttgtgcttg ataccttcta atataatctt ctcttaaatg tgatttttgt ttaaggaact
    14521 ctgaatattt tccatgatat ttcttaatca tgccaaattc tatatcaaga atacctgtag
    14581 aaattttttc taaaaagtca aaatcgtggg aaactacgat aaatgcccca ttaaatgtat
    14641 ttaaataatt tgagagccat tcaacatgtt ctttatctaa aaagtttgtt ggttcatcta
    14701 ataatagaac atcatggtta gaaagtagta attttgctaa aattaccttt gccctttgac
    14761 ccccactaag ctctccaaca acacgattca ttcctattga atctaatcca agaccatttg
    14821 ctattttatt tatttcatta tctatagaat agaaattact tgctattagt tgttcctgat
    14881 aatctgatgc tttgattaga tattgctcat tttcagatat agcacttttt tgatagagta
    14941 aattcatttc cttttctatt ttataaagtt cttcaaatgc agtgtgtaaa tagagtgata
    15001 ttgttgtatc tctattgatt tctgcatatt ggtctaaatg gcctatattg atattaggtt
    15061 gccacttgat ttctcctgaa tctggaacaa cttctcccaa taaaatttta attaatgtac
    15121 ttttacctgt accattttgg ccaactacac ctatatgttc acctttgaat aaatcaaagt
    15181 tagctttctc atacagtgat ttatccataa aacattgtga taaattagtt acttttaata
    15241 aactcataat tcaaactcct tttcttttat taaaaaattt atggaaataa aaaaagcaga
    15301 ggaatggcat tgtaaaatgc cgacctctgc ttttggaaaa acaaaaggtc atagacaagc
    15361 attgttgtct atgaccagaa aatctatatt atctatcggc aaataggtaa ttttaaataa
    15421 gcatagccaa aaaactatac taaacctata tttgccgatg aagatagaaa gctttaagct
    15481 ttcagattaa cctctacaca acgcttcatc gtatacattt gtgtagagtt aagttttcta
    15541 acaagtttaa ctaattctat ataaatcata attattcaca tcctaatctt agtattaatt
    15601 ctagtttatc tatatttaaa ttgtactttt gtactttgtg gattatctta tttatttaaa
    15661 atatgtttta ttaattatag cttaagttat gttatcataa tacttagtta tagtcaataa
    15721 gaaagttata tttactatta ttatattaat ttagtaaatc tatcattaca aagttatttg
    15781 aagactcgct atcaataaaa ttttatagtc tataagaaaa ttaagattat gagttttata
    15841 attttgatat agggatacta ttttcataaa tattacaaca tgtagaagta tatgtctagt
    15901 attgttgaat tttca
//
LOCUS       DAFKMX010000094        15790 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFKMX010000094 DAFKMX010000000
VERSION     DAFKMX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.094"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(132..866)
                     /locus_tag="LZB08_002272"
     CDS             complement(132..866)
                     /locus_tag="LZB08_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBM7315770.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            992..1708
                     /locus_tag="LZB08_002273"
     CDS             992..1708
                     /locus_tag="LZB08_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBM7315771.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1842..2534
                     /locus_tag="LZB08_002274"
     CDS             1842..2534
                     /locus_tag="LZB08_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315772.1"
                     /translation="MNNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2524..3705
                     /locus_tag="LZB08_002275"
     CDS             2524..3705
                     /locus_tag="LZB08_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBM7315773.1"
                     /translation="MKNKSLLNHMFRPILDIIISCALTSMLILTSMYIIKSLYFNSGI
                     SVINQLVYQLRDLRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTMFEVQLPIIQS
                     A"
     gene            3987..4442
                     /locus_tag="LZB08_002276"
     CDS             3987..4442
                     /locus_tag="LZB08_002276"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBM7315774.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4432..5157
                     /locus_tag="LZB08_002277"
     CDS             4432..5157
                     /locus_tag="LZB08_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBM7315775.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5129..7510
                     /locus_tag="LZB08_002278"
     CDS             5129..7510
                     /locus_tag="LZB08_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBM7315776.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREVQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7715..8944)
                     /locus_tag="LZB08_002279"
     CDS             complement(7715..8944)
                     /locus_tag="LZB08_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBM7315777.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9545..10459
                     /locus_tag="LZB08_002280"
     CDS             9545..10459
                     /locus_tag="LZB08_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315778.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10483..11118
                     /locus_tag="LZB08_002281"
     CDS             10483..11118
                     /locus_tag="LZB08_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBM7315779.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11494..12210
                     /gene="pyrF"
                     /locus_tag="LZB08_002282"
     CDS             11494..12210
                     /gene="pyrF"
                     /locus_tag="LZB08_002282"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBM7315780.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12236..13279
                     /gene="carA"
                     /locus_tag="LZB08_002283"
     CDS             12236..13279
                     /gene="carA"
                     /locus_tag="LZB08_002283"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBM7315781.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13332..>15790
                     /gene="carB"
                     /locus_tag="LZB08_002284"
     CDS             13332..>15790
                     /gene="carB"
                     /locus_tag="LZB08_002284"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBM7315782.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMV"
BASE COUNT         6373 a         1742 c         2838 g         4837 t
ORIGIN      
        1 ctattaagaa ctattaaaat atagattaaa gataataaca tataataaat aaaaaagtaa
       61 gattgtgtag attatataaa gattatttac aattaataca atcttgcttt tttatttaaa
      121 aattttaatt attatttaaa attttttatc ttctctttat cccatataaa caatttctca
      181 gaattattta aagcaccaaa tacatttctt ttaaaatcag gcatctcttt atttaaatta
      241 gcaataagat tttttatgta ctctctccta gtatgaccat ttgcagggca gggatttgtg
      301 ataataggat agttagaatc ttttgaaatt ttcttagtca tatactcttc tatgtaaacc
      361 ataggtctta taactgttaa atcctgtctg tctaggtatg tctttgggga aaaacaattt
      421 actctaccct cataaaacat agacattaaa aatgtctcaa ttgcatcatc tttatgatga
      481 ccaagagcta ctttattaca tccaagtttt ttagcattat cgttcaaagc acctcttctt
      541 aaattagcac acaatgcaca aggattcttt tcttgtctta tatcaaatac aatttctttt
      601 atgtcagttt gaaattcata aaaaggaaca tttatttctt tacacaattt atctagtgga
      661 gaattatcaa cacctccagt atttaatgtt atggctatca attcaaagtt ttgtggagaa
      721 aatctttgat aacttttaag tatatgaagt agagttaaac tatcttttcc accagaaagt
      781 ccaaccgcaa ttttatcatt ttcttgtatc atatcaaaat cttgtatagc cttacgagct
      841 ttacttagaa gtttttgtat agacatttta tcaccatcct ttaaaaatat taaatttaag
      901 atttatttaa gatttactta taaaatactt aagtcttata ctctaaaatt aacattacag
      961 acaaacataa caaaaaggag aagatttaat tatgaaaaaa tttaaattaa taggagccat
     1021 tatattagta gtatttataa gtgtatttat atctaaagat agattactta agaaagataa
     1081 agatccaata gtagctgctc aacatgattc agaacaagat gttgaggcaa attctcaaaa
     1141 taaaaatcac tcaaatagta tatcaaatat attattggta aacaagacaa atggaattag
     1201 caaaaactat actcctgaaa atattactaa ggtaaacata ccttttgttg aagaagcaac
     1261 agaggaagaa aaacaaatgg caggagaacc tgcaaaggca gttgaagatt tagttaagca
     1321 agctaattca gaaggaatac agtttttagg gtcttcagca tacagatctt atgatactca
     1381 actggatact tatacaagaa gggtaaaatc tcagggacga gaaaaggcag atgcatatgt
     1441 agcaaagcct ggttacagtg agcaccaaac tggactttgt attgatttaa caaatccaga
     1501 aagatggttt gttggaagta caaaagaagc taaatggttg gcagaaaatg ctcacaagtt
     1561 tggatttata ataagatatc ctgaaggtaa agaagatata acaggtacag catatgaacc
     1621 atggcatatt agatatgttg gtaaggatgc tgccgaggaa atttattcaa aaggattaac
     1681 tttagaagaa tatttacaaa ataaataata taaatgtgta aataatttgt attatattgg
     1741 aaatttagaa attaataaat ttatagatta cactaactaa aatatagtat aattttatat
     1801 gaataaagct atatacaaag attaagttag gatgtgtaaa aatgaataat acaattcttg
     1861 ttgtggatga tgaaaaggaa ataagagacc ttatagagat atatctttta aatagtggat
     1921 ataatgtaat taaagctgaa gatggaaaag aggctctaga tattttaaaa aaggaaaata
     1981 ttcatttaat gatattggat ataatgatgc ctaaaatgga tgggatagaa gtttgtagaa
     2041 aagttagaga aagtttgaat ataccaatac ttatgttaag tgcaaaatca gaagacatgg
     2101 ataaaataca agggataatg actggtgcag atgactattt gacaaaacca tttaacccac
     2161 ttgaacttac tgtgagagtg aagtcgctga ttagaagagc atattatttt agtggtgcta
     2221 ataatgaaga taaagatgtc ataaaggttg gagtagtaat tatagataag gaaaaacata
     2281 gtgtaacagt ggctggaaat gaaataattt tgacatcaag agaatttgat atattatatt
     2341 tactagcaaa taataaaggc agagtatata gtacagaaga aatatttgaa aaggtatgga
     2401 aagagaaata ttaccaatct aataatactg taatggttca tatgagcaga ataagagata
     2461 aaattgaaaa atatacagat ggagaaaaaa taattcatac tgtatgggga gttggatata
     2521 aaattgaaaa ataaaagttt attaaatcat atgtttcgac ccatattaga tattataata
     2581 agctgtgctt taacttcaat gcttatttta acaagtatgt atataataaa atcattatat
     2641 ttcaatagtg ggatatctgt aattaatcaa cttgtatatc agttaagaga tctcagaagt
     2701 cttataggag agagtttatt ttatccattt ttatttacaa tcacatcatt agcaatttat
     2761 tccatgatat cgtacaagag aaataagcaa attgtagatt ttataagaaa gactaataga
     2821 aaaaatcaaa aattagaaat cattaaaagt gctgtaaatt taatggatga aggtaactta
     2881 gaaaaaagta tagatattga gtatgattta gatatattaa aggataaaga aaaggatgat
     2941 attgatgaac tagcacataa aatcaataat atagtccatc aacttagaaa tataactatt
     3001 gaagagagac aagctcagca gacaaaaaca gatttaataa caaatgtgtc acatgactta
     3061 aggacacctt tgacatccat aatgggttat ttaggagtga tagaagaagg taagtataaa
     3121 gatgaagttc agatgatgta ttatgttgat attgcatatg aaaaatcgaa aaatttaaat
     3181 gttcttataa atgatttgtt tgaattgact aaaatgcaaa ataatacaat aaaattaaac
     3241 aaaatagaaa taaatttagt agagttatta agtcaagtag tatctcaatt tgaaatttat
     3301 tttaaacaag aatttatggt tagtagaatt aattttaatg aagaaaaatt aattgtaaaa
     3361 gctgatccaa ataaattggt aagagcattt gaaaatctta taactaatgc tgtgaaatat
     3421 ggaaaagatg ggcattatgt agatatagtc acagagaaaa aggaagatat ggcggttgta
     3481 aaagtgataa attatgggga accaattcca gtgttagatt taccaaatat atttgataga
     3541 ttttatagag ttgaaaaatc gagaaataga aatgttggag gttctggatt gggtcttgcg
     3601 attacaaaaa atattgtaaa tttacatgat ggagaaatta ctgtttcaag cgataaatat
     3661 caaactatgt ttgaagtaca actgccaata atacaatcag cttagttata aaataaaagt
     3721 acgattatgt gatataaata ttataacaat aaagtgttta caaaaataaa aaagtaatat
     3781 ataatttaag tcagtttaat aagatactta tcaagagtta ggggagagag ttagctcagt
     3841 gatcctatac caacctttta tcaggtggtc ctgctaacaa cgatgagatg acttgaaata
     3901 atatactctt cttatcaata atagatagga agagtttttt ttagaattaa tgtaacaaat
     3961 tttaaagtat aatggaggat taaataatgg aaaaagtaga aagttttaag ctagaccata
     4021 caaaagtaaa agctcctttt gtaagaaagt gtagtgtact tgatggagta aagggagata
     4081 aagtaactaa atttgacttg agatttttac agccaaatgt agaaagtttt ggaactgctg
     4141 ctatgcacgg tttagagcat cttttagcta cttatttaag agatacatta gatggtgtaa
     4201 tagacttatc tccaatggga tgtagaacag gattttattt aatactatgg ggagatgtag
     4261 atgccaaaac agttaagatt ggtttagaag aagcattaaa aaaagttctt gaatcagata
     4321 aaatgccagc agctacagct atagaatgtg gaaactacag agacttatct ttatttgggg
     4381 caaaggaata tgcaaaagat gttttagata aaggattttc tttaaatata tatggagaat
     4441 aatttaattg gaagagaaga tataagcata aatcaaagtg aagttttaag atatttacaa
     4501 tacagaggtc aagagataga taaagacctt aaatatacta ttgatgaatg tgtagctata
     4561 actaaaaata aaataaatcc tagatattta tctaggattt atcctattaa aataaataaa
     4621 gataatgttg tagaattaga gggaacaaat ttaacattag agagtaaaga tgtatatgaa
     4681 cttttaaaag aatgtaatga gtgtgtaatt atggcagcta ctatagggat agatatagaa
     4741 aaagaaataa gacgacactc ttattcaaat ttaacaaaag gactaataat tgattcatgt
     4801 ggaacaacag caatagaaga agtgtgtgac attgttcaaa ataatttaga aaaggttttt
     4861 ttaaagcaag ataaatttat aactatgcga tatagcccag gatatggaga tttaccaata
     4921 gaaaaaaata tagatatatt aaacgttttg aatgctcaaa aacaaatagg tctaacaatt
     4981 acttctaatg gaattatgat accgagaaag tcagtagtgg ctattatagg tatttctaat
     5041 acaagaataa ataaatctaa gaaagaaaag tcgtgtgaaa attgtaaaaa ctatagaaac
     5101 tgtacatata gaaaaggagc gaactcttgt ggaaataaga caatatctga aaaataatat
     5161 attaatattt gatggtgcaa tgggaacaat gcttcaacaa aaagggttaa agttaggtga
     5221 aaatccagaa gtatttggtc ttcaaaatcc agataaactt atagaaatac atacagcata
     5281 cttagaggca ggttcgaatg taattcttac aaatactttt ggatgtaatg aactaaaatt
     5341 agatagtaag tatactgtag aagaagtgat tgacaatgct gttttggtgg ctagaaaagc
     5401 tatagaaaat gtagataata ctaaaccaag atatgtagct ttagatattg gtcctatagg
     5461 agaaatgcta gagcctatgg gtactttaag ttttgataaa gcatatgaaa tatttaaaag
     5521 acaagtacta caaggcgtta aatctggagt tgatgtaata gtaatagaga ctatgatgga
     5581 tttatatgaa gctaaagtag ctgtattagc agcaaaagaa aactctgatt tacctatttt
     5641 ctgtactatg acatttgatg aaggtggaag aagttttact ggatgtatgc cagaatgtat
     5701 ggtggctact atagaaggtc taggtgtaga tgcaatagga gttaattgtt cattaggacc
     5761 taaacaactt ttaccaatag ttgaaaaaat agcatcaaga gctacagtcc ctgttatggt
     5821 tcaagcaaat gcaggtcttc caaatatagt tgatggagag gctatatatg atgttgatgc
     5881 aaaagaattt tttgaagggg ttaaaaagtt cgttgaagtt ggtgctacta taataggtgg
     5941 atgttgtggt acgaatccaa gttttataaa agaaatatct gagaatataa attctgttac
     6001 aaagggatgt atcgagaaaa ttgataaatg tgtagtctgt tcaccatcaa aatttgtgga
     6061 agtacaatcg cctacagttg ttggagaaag attgaatccc acaggtagaa aatctctaca
     6121 agaagcatta aaaaatgaaa atgttgatta tgctataaat ttaggtttag aacaagtaaa
     6181 tgctggagca caaatacttg gagtaaatgt tggtcttcca gagattgatg aaaaaaaact
     6241 aatgccaaaa ttaataagag aggttcaagc agtagtagat acacctttac aagttgattc
     6301 ttccaatgta gaggccttag agcaaggact tagatattat aatggtagaa caatagtaaa
     6361 ctctgtaaat ggcaaagagg agtctttaga aagtatatta ccaatagtta aaaagtatgg
     6421 ttcatgtgtg gttggactta cacttgatga aaaggggata cctaaaaagg ctgaagaaag
     6481 atttgaaata gcaaaaagga tagttaatag agcagtttct tatgggataa aaccaaaaga
     6541 tatatttata gactgtttat cattaactgt ttcagcacaa caagaagaag caatagaaac
     6601 tataaaagct ataacaatgg taaagacact aggagtaaaa actatattag gtgtttcaaa
     6661 tatttcattt ggtcttccaa ataggaaagc acttaatgct tcttttctaa cattagcttt
     6721 aggggcaggg cttgatttag caattataaa tccaaatgaa tatagtatga tggaagctat
     6781 caattctttt aaaatattaa ataacacaga taaaggatgt ataaactata taaatcaata
     6841 tagcaatata aataattcaa aaaaatcgga ttcatctaca aagattgata aagatttgcc
     6901 cttggatatt ttagttgaaa gaggattaaa agatgaagca aaaaatgtaa cactgaatct
     6961 tttaaaagag aaggatgaaa attatatatt agatgagatt ttaattcctg ctcttgataa
     7021 agtaggaaaa agatatgata gtggagatat atttttacca caattgattc aatcagcaga
     7081 aacggtaaaa gtttcattaa atacaataaa ggaaacttta ttaagtaaaa gtagcaataa
     7141 tgtatccaaa ggaaaaataa tagttgctac agtaaaaggt gatatccatg atattgggaa
     7201 aaatatagta aaaataatgc tagaaaatta tggatatgaa gttatagatt taggtaaaga
     7261 tgtccctatt gaagaagtag tggaagttgc taaaaagaga aatataaaat tagtaggatt
     7321 aagtgcactt atgactacga ctgttcaaag tatgaaagat acaattcagg cactgagaga
     7381 taatgagata aatgcaaagg tatttgtagg aggtgcagtt ttgacagaag aatacgctga
     7441 agaaatgggt gctgattatt attcaaagga tgcaaagtct gctgttgaaa tagcaaagtt
     7501 aaatttttag catataaatt aaaataaagg ttttactaaa taggatattt tatatataat
     7561 ataaatataa gttttagata cttatgttta aaacaatagg aattaatcta taataatgag
     7621 attaattaat ataaaaaata tttatcatca acattaaaaa gggctgggaa gtctggtata
     7681 acttccatca gcccttttta gttaaaatca tatgttaaaa agcccaatta ccatttttaa
     7741 aaatctgtat ttgctttcca tcataagtct caccaataat atccatatca gcagaaccaa
     7801 tcataaaatc tacatgtgtt aaactgtcat ttacaccatg ttcatccaat tcttcatctt
     7861 ttaaattatt tccaccttcc agacaagtct tataagcaga accaattgca aagtggcaag
     7921 aagcattttc atcatataga gtattataaa aaattatatc cgtattggat attggtgagt
     7981 tgtatggaac aagagcgact tctcctaaat aatgagagcc ttcatccgtt tcaataagtt
     8041 tttctagtgt atctagtccc ttttcagcag agaaatctat tattttacca tctttaaatg
     8101 ttaagaagaa attgtcaatt atgtttccac cataaacaag aggttttgta ctataaacag
     8161 ttccatttac tttaaactta tgtggcattc caaaaatctc ttcagttgga atattcggat
     8221 taaaacttat tccattaggg tctttggatg ctccacttag ccaaacatga ccctttggca
     8281 agtctagagt caaatcagtt tttgaggatt tatattttaa agttttaaag ttatttttat
     8341 ttagaaagtc cattttagat ttaagatttt cattatgttc ttcccatgct tttatagggt
     8401 cctgaccatc tactcttgaa catttaaata tagcatccca cagtttttct attgctttat
     8461 cttctgataa atttggaaac actttttttg cccaatcttt tgttggcaca gagactatac
     8521 tccatttaca tttatcagtt agagtgtaag aacgccattc ttttaaagct tttccagatg
     8581 ccttctgaaa atttgcaact ctttcagggt caacatcttt taataaatct ggattttggg
     8641 catagactga taaaaatgcc ccaccttctt ttgctatatc tacatattgt tctgctgtcc
     8701 atttggggaa ctcatgaaat gcttcatctg gagcatataa gtatttaata agagtacttt
     8761 cttcatcaga ccactctata taaacatttt tagcaccaca tttatatgct tctttaagtg
     8821 catttcttgc aaaatcagca cattctattg gtgattttat aagtagaatt tgacctggct
     8881 gtatgttaac tccaactttt attgctaatt cagcgtattt ttctaaattt ctatttaaat
     8941 tcatatttat cctcctatat ttttaatatt gaataactta taaagtatta taacattact
     9001 tatgttttta atattgaaca acttataaaa tattataact ttacttagat tttgggtcat
     9061 attcaaaata tttatacttt atagattgag aaataaaaaa aatattttaa ttaaaacaaa
     9121 ttatgaggat aacaaactta tttaagcagt atgttttttg ttgataataa tattaaagta
     9181 gaaataaaaa tactttttta gtgaaaagct atacaagaat accgaaaaaa acttataata
     9241 taataatgag atagatattt ataaatgaaa aaaaattttt ctggcaaagc ttaattaaaa
     9301 aggtaatagc tagtgttgtt atttaaatta gcatagaaaa tcaaataaaa aaattatgct
     9361 aatgtataga cattgataag gtagaattta agccttttga atgctatatt aaactagagt
     9421 agatattctt atgaatttca aaatagggca ggaggaaaaa ggaaataaaa ctataaaaaa
     9481 tataaagtat ttatataaat ttatagagtt tatacgtcag aagatttcag ttaggagggc
     9541 ataaatggag ctttatgaag ccatttttta tagaaaatct gtaagaaatt tttccagtaa
     9601 gaagataaaa aagccattga tggaagaaat aaaaagaagt tgtaaaaatt tagaatactt
     9661 aaatgaagat ttaaatatta aagcacatgt tgttgataga ggtcatataa tacatttttt
     9721 gatgggtaag gaatgtaaag taaaggcacc tcattatata gtagttacat cagacaaagg
     9781 tgaggactat ttacagaata taggatttgc tacagaaggt gtagttttgc gccttacaac
     9841 attgggaata gctacttgct ggttggaagg aaacttaaag agagaagata tcttggaatt
     9901 tgtagatatt gggaataaga attttgaaga agatgaggaa gatgatttag attatctagc
     9961 tggaataaaa acagaagaag atgatgaaaa caaaatagaa aatccttata taataatagc
    10021 atttggatat ccagcaaaaa atgaagagtt gtttaggacg agatatgcta gacctgatag
    10081 aaagccagta aaagatatga taaataagat aaataggaat agaaagtgga aagatatctt
    10141 agaattgaca agaagagctc catctgtaaa aaattctcaa ccgtggtatt tccataagga
    10201 tgaacgaggt cttcatttat ttgaaaagag acctaagaaa cactgtgaag atatgaataa
    10261 ggttagctta ggtgtagcct taagacattt tgatatagct tgtataaaaa ataaaataga
    10321 tgtttcatat gaaaaacttc ctataaggaa taaaatagga aaatcatatt ttataactgt
    10381 agtcgagcat gtgaaaccag aagaagaaac ccaagaagaa aatgtaactc tagaaaaaga
    10441 agaatcgcaa gatgaataaa attttggaag gggatattgg atatgtataa gtgtggaaaa
    10501 tgtaatctta gaggctgttc taatggaaat atggataaat taatgaagat atgtccaact
    10561 agagaaattg aactacaaca aaaagcaaag caactttatg aagaagaaga gaatagaaaa
    10621 atagcttata atgctggatt agttgaagca gaggggtatt gtcaatatac tagactaaaa
    10681 gagattatag agtttgctaa aaagtgtggt tataaaaaga ttggcatagc attttgtatt
    10741 ggactaaaaa gagaaatgca aacagtgcaa aaaatatttg aacatcatgg gtttgaagta
    10801 ttttctgtta tatgtaaaaa tggggcaatg cctaagagta caattggact agaagcatct
    10861 caaacaatat caaagtgttc agatgaggta atgtgcaatc caataggaca agctttattt
    10921 ttaaatgaag ctaaggttga ccttaatgta attcttggat tatgtgttgg tcacgatact
    10981 ttagccatga aatatatgga agctcctata acaactttag ttgtcaaaga tagagtaact
    11041 gggcataatc cagttgctgc aatatataat gcagaatcat attttgagaa aaaattgttt
    11101 gataaagatg aagaataatt caaagactta ttgacataaa aagcaattag tggtttaatt
    11161 ataaaaaaga taaaacttta aattatccta tagatacata aagttatata aataaagaaa
    11221 aatattacag ctaaattcct taaagtagtc cagagagact aaagggttat agataatgtt
    11281 agtatataac ctctagtaaa tcttctagag gtttttgatg agtataaaat aaaaatttaa
    11341 atataataaa gtcttttaaa ctagtccaga gagactggga aagatatctc atagctaatg
    11401 aatagagtta tgtatagata aaatccttat gcctcttttg gtgtgaggat ttttttatga
    11461 ttatttaggc tttattaaaa aggagatgta aaaatgataa atggtaaaga aaaattgata
    11521 gttgcaattg atactgacga atttgataaa gctaaagaac taatagacag attagaagat
    11581 agtgtagaca tatttaaagt agggctagag caatacgttg ccacaaaagg taaaacaata
    11641 gattatctta aagaaaaagg taaaaaaata tttttagacc ttaaattcca tgatatacca
    11701 aatactatga aaagtgcagt gaaagctgca gtgagagata atgtttggtt aatgacaata
    11761 catgtatcag atatggaagg aatgagacaa tgtgcactaa ttgctaggga agaagcagaa
    11821 agattaaata tagaaaagcc attagtagtt ggagtaacag ttttaacttc attaagcaac
    11881 caagatttac aggacatagg atgtaatatg acaacagaag agttagctat aaagagagct
    11941 aaactggcaa aagaatcagg aatagatggg gttgtttgtt cagcgcaaga agtagataaa
    12001 atagtagaag catgtggaac tgattttata acagtttgcc caggaataag acctaaatca
    12061 gcagaggttg gagaccaaaa aagagttgta actccatcag atgcaataaa aaaaggtgct
    12121 cattatttag ttgttggaag acctataaca aaagcagaaa atccaagaga atcagctatt
    12181 aatattgtaa gagaaataga aaatgcttag ataaaatatt tgggaggtag cttacatgaa
    12241 ggggaaatta attttagaag atggaacagt atttgaagga aaagcatttg gatatctaaa
    12301 ggaagctatt ggagaagttg tatttaatac ttcaatggct gggtatggag aactattgac
    12361 agatgcaact tattatggac aaatagttac tatgacttat ccacttatag gaaactatgg
    12421 aataaattta gaagatgttg aatcaaataa ggtgcaagta aaaggactta ttgtaagaga
    12481 aaaaagtgat aacccaaata attttagatg tgaaatggat atagacacat atctaaagca
    12541 aaataaggta ataggacttg aaggaataga tacaagagca ttaactaaaa ttattagaaa
    12601 caatggaact aagagaggaa ttataacttt agaaaatgta tcattacaag atgttcagtc
    12661 taaattagaa gagcattgta acaaagattc agtaaaaaat gttacaagaa aagaaattga
    12721 aaatataaaa ggtactggag ctaaagttgt agttatagac tttggtgtta aaaatagtgt
    12781 actagagtct cttaaggctt gtaactgtga tataactata gtaccagcaa tgactacagt
    12841 agaagaaatt ttaaatataa atcctgactt aatcttatta tctaatggcc caggaaatcc
    12901 agaggatata aatgatatag ttgaaaatat aaaaaaatta ataggcaaaa agccattgac
    12961 aggaataggt ttaggacatc aaattttagc attggcatta ggaggaaaga cttcaaagct
    13021 tgtatttgga catagaggag gtaatcaccc agttaaatgt ttagaaactg gaaaggtatt
    13081 tataacttct caaaatcatg gatataatgt ttcagaaatg ccagaaaata cagaagtaac
    13141 tcacataaat ttaaatgaca atactataga aggaatgaga cataaggaat taccaatata
    13201 tagtgttcag tacattccag aatacacaac aaaagaagat ttagattgta tatttaataa
    13261 attcttagca ttaatataga tttagtatta tttacagata gtaaaaacca tttagataag
    13321 gagaaaaatt tatgcctaaa ttagatagta taaagaaaac tttagtatta gggtcaggac
    13381 caataataat aggtcaagct gcagagtttg actattcagg aacacaagct tgccagtctt
    13441 tgaaagagga agggattgaa gttgtattaa taaatagtaa tccagccact ataatgactg
    13501 ataaagaagt ggcagataaa atatatatag aaccattaac aatagaattt atagaaaaaa
    13561 taatagaaaa agaaagacca gacagtttac ttgcaggtat gggtggacaa acagggctaa
    13621 acctagcagt tgaactttat gaaaaaggca tactagataa atatggagta aagataatag
    13681 gtacttcagt agaatctatc aaaaaaggtg aagatagaga tatatttaga gaagtaatga
    13741 aagaaataaa tcagcctgta atagttagtg atatagttac agatttacaa gcaggtcttg
    13801 attatgcact tacaattgga tatccagtag ttgtaagacc agcatacaca ctaggtggaa
    13861 ctggtggggg tatagctgat aatgaggaag aattaagaga aatactttca catggtctac
    13921 aattaagtcc tgttggacaa gtattaattg aaaagagtat aaaaggttgg aaagaaatag
    13981 aatatgaggt aataagagat tcaaagggaa attgtatagt tgtatgtaat atggaaaata
    14041 tagacccagt cggagttcat acaggagaca gtatagttgt tgctccaact caaactttaa
    14101 gtaacaaaga atgtgcaatg cttaaaaaag catctttaga tatattaaat gcagtagaag
    14161 ttcaaggtgg ttgtaacgtt caatttgcac ttaatccaca tagttttgaa tatgcagtta
    14221 tagagataaa tccaagagtt agtagatcat cagctttagc atcaaaagct acaggatatc
    14281 caatagctaa agtttcatca aaaatagctt taggttatac tttagatgag atagaaaatg
    14341 cagtaactaa aaaaacttat gcttgttttg aaccaactat agattatgtg gtggcaaaaa
    14401 taccaaaatg gccttttgat aaatttaaaa aagcaaatag aaaattagga actaagatga
    14461 tggcaactgg tgaaattatg agtataggaa gtaattttga ggctgctatt cttaaaggga
    14521 taagatcact tgaaacagga aaatattcat tagttcacac accatctgag gagagaagca
    14581 ttgaggaatt aaagaaaaga gttgtagtac ctgatgatga gagactattt gatttagctg
    14641 aaatgataag aagaggctat aaagtagaaa tgatagaaca aattacaggt atagataaat
    14701 ggtttataaa taaatttaaa tggatagtag agcaagaaga aaaattaaaa gttatgaaaa
    14761 ttgaagattt aactaaagac tatcttcttg aattaaagaa aaagggattc tctgataaag
    14821 gtatatctga cctaatgaag ataagtccag aaaaattata tgaattgaga agtctataca
    14881 atataaaacc agcatataaa atggttgata cttgtgcagg tgagatagat gctatatctc
    14941 cttactatta ttcaacttat gagcaatatg atgaggtagt tgttagtgat aagaaaaagg
    15001 tgatagtatt aggttctggt ccaataagaa taggtcaagg tatagaattt gactattgtt
    15061 cagttcactg tgtaaaatct ttaagaaaaa tggacataga aactataatc ataaacaata
    15121 accctgaaac tgtaagtaca gactttgata catcagataa gctgtatttt gaaccattaa
    15181 cagaagaaga agttttaagt ataatagaaa aagaaaagcc agaaggtgta atactacagt
    15241 ttggtggaca aacagctata aaattggcta agtttttaca tgaaaaaaat atacctatac
    15301 tgggaacaga ttttgatgat atagatgcag cagaagatag agaaaaattt gatgatttac
    15361 tagagagact agatataaat agaccaaaag gaaagggtgt atggtcactt aacgaaggta
    15421 ttgaagttgc taatgagtta ggttatccag tattagttag accatcttat gtacttggtg
    15481 gtcaaggtat ggaaataact tataacgaag aaaaattatc tcaataccta caagatgcat
    15541 ttgatagaga ccataaaaat ccagtactta tagataaata tctaactggt agagaaatag
    15601 aagtagatgc tatatgtgat aaggaagata tattgatacc tggtataatg gaacatttag
    15661 aaagagctgg agttcactct ggagatagta caacaatgta tccaagtcaa aatatatctg
    15721 atgaaataaa agaaaagata gtagaataca ctaaaaaaat agctttagac cttaatgtgc
    15781 ttggtatggt
//
LOCUS       DAFKMX010000095        15746 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFKMX010000095 DAFKMX010000000
VERSION     DAFKMX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.095"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            592..4734
                     /locus_tag="LZB08_002285"
     CDS             592..4734
                     /locus_tag="LZB08_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBM7315783.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDAVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVNFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGN
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVASA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENVQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
     gene            5191..5538
                     /locus_tag="LZB08_002286"
     CDS             5191..5538
                     /locus_tag="LZB08_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBM7315784.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(5688..6188)
                     /locus_tag="LZB08_002287"
                     /pseudo
     CDS             complement(5688..6188)
                     /locus_tag="LZB08_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(6215..7720)
                     /locus_tag="LZB08_002288"
     CDS             complement(6215..7720)
                     /locus_tag="LZB08_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBM7315785.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEEIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(7920..8774)
                     /locus_tag="LZB08_002289"
     CDS             complement(7920..8774)
                     /locus_tag="LZB08_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7315786.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQPQGVLGISICNNPEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            9159..9440
                     /locus_tag="LZB08_002290"
     CDS             9159..9440
                     /locus_tag="LZB08_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315787.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            9590..10438
                     /locus_tag="LZB08_002291"
     CDS             9590..10438
                     /locus_tag="LZB08_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBM7315788.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            10490..11011
                     /locus_tag="LZB08_002292"
     CDS             10490..11011
                     /locus_tag="LZB08_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315789.1"
                     /translation="MIPLLILGLLKQSPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYENEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            11362..11895
                     /locus_tag="LZB08_002293"
     CDS             11362..11895
                     /locus_tag="LZB08_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBM7315790.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            11892..12653
                     /locus_tag="LZB08_002294"
     CDS             11892..12653
                     /locus_tag="LZB08_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315791.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHKLTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVILSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            12691..13557
                     /locus_tag="LZB08_002295"
     CDS             12691..13557
                     /locus_tag="LZB08_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315792.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            13578..14819
                     /locus_tag="LZB08_002296"
     CDS             13578..14819
                     /locus_tag="LZB08_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HBM7315793.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGNYTQKEKDFWLSKNKDIKEPYLNGYYQGWEYFLLARDLLIFTIMSVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(15043..15615)
                     /locus_tag="LZB08_002297"
     CDS             complement(15043..15615)
                     /locus_tag="LZB08_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315794.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         5899 a         2087 c         2311 g         5449 t
ORIGIN      
        1 taagcattta agtagatttt aagcttgatt tgattagaaa ggtacaaact atcaccgaaa
       61 aaaattaaac ccatttaaaa agcaatataa aagcttgtta tttttgagaa aaatagaccc
      121 tctaaactca attagataaa aataaaatac agaaaattat atctctaaat atctcaagta
      181 attaatttta aattttacga tattttgttt gatgatttat aatataaaag catactctta
      241 atctgctcaa attaaaaaga ataaaaaata acaaaaaaaa tcactgacat ttcactgaca
      301 aatggtaatt ttgttattat aaatacattg aaaatacttg attattgaaa aatggcataa
      361 aataatacat tttatgccat ttactattat ttgatagttt ttaacttatg acataagata
      421 ttttttaaca tatacaataa aatataaaaa aattgatatt gcaaataaaa aaaatacata
      481 ttagatgtta tagaaaaaaa gcgcacttat aggcttaaaa tgtcacataa aagagtttta
      541 aagaataata tattatgaac acaaattttg taatcacaaa ggagtgataa tatggcaaca
      601 gtatcaggac aaatagtttt tgatagaaat agaagtgcta ccattgattc tggagactca
      661 ggaatagcca atataccagt tgtattacaa gatatagcaa ctggagttag acttgtggtt
      721 ctcacagatg catcaggaaa ttatgttttt actaatgttc caaatggtaa ctatagaatt
      781 gtagaagctt ttggaactac aggaggagta ccaacaccag ggaattttgc aacagcgata
      841 gtaggtccag ttccaattgc agaaatgcca cctattagtt ctgtgacaaa tcctcctatt
      901 ggttcaacaa acttagatgg tacaacacct aacacactat taataacagt agcaggttca
      961 gatataacaa atcaaaatat attaaatggg cctgtgattt atacacctat agagaatata
     1021 ttggattctt gcgtatctgt atctaatata aaccttatta cagatgcaga taacggaaca
     1081 ttcggttttt ttccaccagg tacaccaagt aatacaggac ctgaaataga accttaccca
     1141 aatgtgacgc cagattttac gtatgttgta ccagacccaa ctaagtttac acctttagat
     1201 ggagaatata cagtacaaaa tattatgaca aatgcaatga gtaatgttat aggagcttgg
     1261 tggcgtattg ctgaccatac tacaggaaat gaaacaggaa gaatgatggt ggtaaatggt
     1321 tttaatcctg gagctgtatt ttttaaatca acagtacaag ttacacctaa cactaattat
     1381 ttatttagca cttggatttt aaaccttttt aaggtaacag gatatccacc accacagcta
     1441 ggtgtaagaa tatttgacca aaataataat atactatatc aagcaacatt aggagctcaa
     1501 attccagtta atacaaacgc accagaatgg aaagaagtag gtactgtgat taattctcaa
     1561 aataataatc aacttacagt agaatttttt agtgaaggag aaagcgccat tggaaatgac
     1621 tatgctatag atgatgtttc tttaagagaa atagctgttc caacttttac tccaataaaa
     1681 acaagtaaca cagaaactgc taatgtaggt gatataatca catatacagt tacattagat
     1741 aatacttgtg atagcccttt aactaatgta ttttttaggg atttaattcc aaatggttta
     1801 gagtttatac ctggaactgt aacggttgat ggagtatctg tgccaggagt agaccctaat
     1861 acaggtttcc cacttccaga tattggagga ggaacagggg ttgaagtaac ttttgatgct
     1921 gtggttgaaa gtattcctaa cccaaatcct actaataata tagctaatat agattactcc
     1981 tatacacctg tagaaggcgg tataccaaat gatttttcag tagattctaa tccagttcct
     2041 gttgaagtaa tatcagcaga tattgaagtt acaaaattat ctgaacctac tatagtgaat
     2101 ccaggagagg aattaatata cactataaaa gtagtaaata atggaccatt cccatctgaa
     2161 aatgtggttt taacagatga tgtaccagct tctattataa atccagagta ttcattagat
     2221 ggaggagtaa attttcaacc atggactgga agtttaaata tagggacatt agaagtagga
     2281 gagacaagag ttattataat taggggtatt gtaaatccat caacagtagg aattataaca
     2341 aatacagctg tagtatcttc aactacagca gacccaaacc ttaataataa tacatctaca
     2401 atagagacag aagtgaattt attagcagat attttagtaa tgaaaacagc agaacctaat
     2461 tcagctgttc caggtacttt attgagatat actattcaag tagaaaactt aggaccagca
     2521 aatgctgaaa atgtaatatt aaatgatgat attccagctt ctattataaa tccagaatat
     2581 tcattggatg gaggagcaag ttttcaacca tggaatggaa gtcttaatat aggaaattta
     2641 aactctggaa tttcattaac agttttaatt caaggaacag ttagtttaaa ttcttcagaa
     2701 tatatagtaa atacagcaac agtttcttca actacacctg acccagattt aagcaacaat
     2761 atatctacca ttattacacc agttaaccca caagcaggta tttctattat aaaagtagct
     2821 gacgaggatg tagctgttcc aggagaagaa tttgtatata caattgaaat ttttaatgaa
     2881 ggaccatcta atgctacgaa tgtagtttta acagatgata taccagatgt tattttaaat
     2941 ccagaatatt cattggatgg aggagcaact ttccaaccat ggactggaag tcttaatata
     3001 ggtacggttg caccagggca attaataaga attatcataa agggattagt aagttcaact
     3061 gctacaggag atattactaa tacagcagaa gtaagtgcag atgtaccaga accagttacg
     3121 gattcatcaa cagtgacaac tcctatagtt gcatcagcag atatagaagt aataaaaact
     3181 agcaatatgg atacagcagt tccaggagaa acttttagtt atactataac agttacaaat
     3241 ttaggaccat ctgctgcaca aagtatcgtt ttgacagatg atataccaga tgttatttta
     3301 aatccagagt attcattgga tggaggagta actttccaac catggaatgg aaatcttagt
     3361 ataggcactt tggatgctgg agaaataaga agtattataa taagaggaac agtgagccag
     3421 acagcagtag gtacaattat aaatacagca acaataagtt caactacacc tgacccaaat
     3481 ccagataata atacttcaac agatgaaaca gatataagtc cattagcaga tatttctgtg
     3541 ataaaaggca acgaaccagt tgctatacca gggggaagat ttatttatgg aattgaaatt
     3601 gcaaatgcag gaccatcttt tgcagagaac gtaactttaa cagataatat accagcttct
     3661 attttaaatc cagagtactc aatagataat ggagtgactt tccaaccatg gaatggaagc
     3721 cttaatatag gtactttaga tgctggagaa ataagaaata tcataataag gggaacagta
     3781 agtcaaacag caataggcac aattataaat acagcaacag taagttcaac tacacctgac
     3841 ccaaatctaa ataacaatac ttcaacatca gaagcagagg taagttcatc agcagatatt
     3901 tctgtagtaa aacgaagcaa tcagattgtt gttgtaccag gagatgtact agactataca
     3961 attgaggtga gaaatgcagg accgtctact gctcagaacg taactttaac agataatata
     4021 ccagcttcta ttttaagtcc agaatattca atagataatg gagtgacttt tcaaccatgg
     4081 aatggaagtc ttagtatagg cactttagat gctggagaga taagaaatat tataataagg
     4141 ggaatagtaa gtcaaacagc aataggtaca attataaata cagcaacagt aagttcaact
     4201 acacctgacc caaatccaga taataatact tcaacatcag aaatagatat aagttcatca
     4261 gcagatattt ctgtaataaa attagcaaat aaaacagaag catgtgtagg agagcaaata
     4321 gattttgtaa tagtagtatc aaatgcagga ccagaaaatg ttcaaaatgt tactttaata
     4381 gataatgttt cagataagct taagaaagca gtattttcac ttgatagagg tgtaagtttt
     4441 caaccatgga ctggaagtct taatataggt acactaccag ctggaacttt aagagtgatt
     4501 atattaagag gaattataaa atcaacatgt cttgatagat taacaaatat tgcagaagtt
     4561 acttcaacaa caccagattc aaaccttaac aataatatat caagagtgca agtagaaata
     4621 aaacaatgtt gttgtaatga ttgctgttgt aacaactgta gtaattgttg ttgcaatgat
     4681 tgtggttgta aagattgttg ctgtaattgt tgtaaaaatg acttttggtg ttgattttat
     4741 aaaataattt tgtatacctt aaattaaact gaaataaaaa ataaattcac atatgagtaa
     4801 aatattttat aaatattaat aaattttcta tctttgatat ggaccacatt cgtaatacta
     4861 gttattaaat gaagtggtcc tttttaattc attaaaatta tttattctaa ttaaatttta
     4921 aatgtattaa ataattttaa ttttttataa aaaatattga tatgttattt atattaaagt
     4981 ttagatatct aaaaatactt ttagaattta ttatattatg ttaatttttt atatcttata
     5041 aggattgtat ttttctaact ataaaaatag tattatactt atatttaaaa ataaaagaat
     5101 tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa taatagtaaa
     5161 tctataatat agaaaagagg tatttctagt atgcatgaaa ataaaaagaa tctattagac
     5221 ggaagtttag ctcaatataa tgatacagca gttccaaaaa taccagtatt tgctggtaat
     5281 gatataactt cagaagttta ttattttaaa cctaatcaag ttcttaatgc tcatagacac
     5341 cctaatggtg aacaaatatt tgtgttccta aaaggagaag gtaaaatgaa acttggtgag
     5401 catgaatgtg atgtaaaaaa cggcgatact gtatttgtac caacaggaga gtggcatgaa
     5461 attactaatg gaagcaatga agaaatggta gccgttcaaa ttacaaaaat taatgctgga
     5521 gcagaataca gaggatagat atataagtac agttagtact ttaatatcta ttttattata
     5581 taaactttaa tctcaaatat aaaaagagtc ttttaaaagg ctctttttat atttttgtta
     5641 attttcatta taaaaaaaag aaaatatcta gctaaaacta atatatttta tttaatattt
     5701 tttatagcat tgtttgcaaa ctcagtattt acgatatctt tataaggtgc ttccttatct
     5761 agttctttag cttctttcat aacagtcatc aatttcttca aaccttcctc ttttaataca
     5821 ggattttgag accatgcatc tattgattta tatctatcta caactgttgt taaatcttct
     5881 aaagatatat cttcaaagaa aggttgcata gccttagcta cttctttgct agatgcttgt
     5941 tgaatccata attgaccttt ataaacagcg tttgtaaatc tttgaattaa atctttattg
     6001 tttttcatgt atgatctcgt agtagagtat gctgtaaaag gtatttctcc agaatctttt
     6061 cctatagatg taactatata tccactacct tctttttcca ttgctgttgc tgtaggttca
     6121 aatgctgttg taaagtcacc ctctccacct gcaaaagctc cagccattac accaaattgg
     6181 taatcagtct atttataaca tttaaaacat ataattaatc tcttgggata ttagggtaaa
     6241 gtattaaaga aaaatcatca gatttttgat taggttcttt tttatagact gcgtaattta
     6301 ttacactttt aagcaatata ttttttagat aaggttcttt ttcctctgta tattcactta
     6361 aaattttttc aagttgagga acaatttgtg ttttaactgt atttctttcc ttttgcattt
     6421 caatatctat atttgtttta ttaatagctt ctcttgcatt ttctatacga gataagagta
     6481 tttttaaacg ttccccaaat gtattattgt cataaattcc ttgttctaaa aaatcatgta
     6541 gtcgttcttt ttgtttttct aaagtatcca attctttatt taaatttgtc aaggtatgtt
     6601 ctaaagtatt ttcaggttta gagttatctt tttcatcttt taaagatagt ctataattta
     6661 ctaaccattt atttaagacc tctaatattt tattttctaa tatgtatagt ttagtagctt
     6721 tatttttaca attagtacaa accatatatt tctcatatcc atttgatgta gtattagatt
     6781 tggctattaa agtatgatta cagtatccac attttacaat tccagccaat ggatttgtta
     6841 ttttattgtt tggagtaggg gaattaatac gtttttttct tatattttgt gccttattcc
     6901 atatctcttc acttattatt ggttcatgct ttccttttac ctcaatttgt tcctcaattc
     6961 ctcttgttct actagaattt cttttcctct cgactttatt ccatactata tatccacaat
     7021 acactttgtt ttttataata tctcttatac ccttttcata ccaagttcta cctgttgtgg
     7081 tcttataacc aagttcgttt aaataattgg atattttaca tgcaccatct ccttttaaat
     7141 acatatcaaa gattatttta acaacagttg ctttatcttc atttattaca agtactcttt
     7201 ctttattatt taaatattta atatcatacc cataaggagg agcttgagct ataaatttac
     7261 cctcttcaat acttttgatt tttcctcttt gcatacgtct agatattaat tttaattcct
     7321 tacgagccat aaaagcttca aattcagaat actcctcatc aaactcatca tttaaatcat
     7381 acgtttttct aggagtaata attttcgttt tagatttttt aaatgtatct agtattaacc
     7441 cttggtcttg catattacca cgacccaaac ggtcaatatc catcactaaa acagcatcat
     7501 agttattatt tttaatttca cctagtaatt ctagcatctt aggtctatat tctatacttt
     7561 ccccagaaac aagttcttct ttaatttcaa tgacatttaa attttgttct cttgctagtt
     7621 ttaaaagagt tgtcttatgt ctacttagag tttcaaattc acctctatgt tctgcttctt
     7681 catcagctct gcttttgcgt aaataaattg ccacattcat taaatcacac cccttgaata
     7741 tattatattc agtaacagat tgattatatt taaaacacaa aaataaacct tcaaaataca
     7801 atatatattt tttaggttta tttttataca gtaaatttta tagtatattt aaaagcttgt
     7861 aatctaagta aaatatttat actattaatt ttatgtcttc ttcatttttt aattttaaac
     7921 tattttttta ctacaggtat ccatacttca aatttagcat tttgagggtc tggatttaag
     7981 tacagttcaa tatcaggacc attatcatac tcataaccag aagtaggtaa ccattctgtt
     8041 acaatacgct tttctagttc ttgtattgct tgtggacatt gtccttctcc agaaaatatt
     8101 gcccaagtaa aagctggaac agtgtattct tctaatgtat tatctataga ttttgtactt
     8161 gaaacagata taaaatactt ccattcttct ggattattac aaatacttat acctaataca
     8221 ccttgaggtt gactatccat catagggatt aatttttgaa gcgttccatc taaagcaacc
     8281 ttttgccaca tttggggaac aatttcaaaa ttcttttcta gttcattgtg aagtggctga
     8341 gatacaccaa ctatacgaaa tgcttctttc ttttcaattc tataattcat ttcttcggct
     8401 cctttaatac taattttaaa gctgattgga ggaaaggctt ttaaagttgt tatttctctt
     8461 ttttttataa ctgatggggc aacaccatga atacttttaa atgcacgatt gaatgctgtt
     8521 ggagaagagt atccatattt taaagccaca tcaattatct ttttatcatc attttgtaat
     8581 tctacagctg ctaaagacat ccgtctacga cgaatatatt cagaaagtgg tacatctgcc
     8641 atataagcaa acattctttg aaaatgatat gtagaacaac aagctatttt tgccacttgt
     8701 tcatactcaa tttcttttgt tatgttttct tcaatatagt taattgcttt atttaatctt
     8761 tcaatccatt ccataaaatc agctccttgt aatttatata atatctattt taaaatagtt
     8821 aatcctctct ttctatgcac taattaacta gcttaataaa aaatgaacat gagaaagtct
     8881 gtaattaact acaaaaatga acaaatagtg ttgtttttca cattaaagta tatcacagat
     8941 tagtatctaa tttttatata agataacagt tttaatttat tatgaaattt ttataatatt
     9001 ttaggttaga catttaatta tatataacat tttatttata gttaactatt tttgaagttt
     9061 acccttaatc aagcaacact tagttattca attataaata atgattgaga ataggacaat
     9121 ttttaagata cataaaatat aaagaggagg ggatttaaat gccaaatata gaaaaagact
     9181 tagatggtac aactactata acatacctca acgacgatat aggtgttcca acagacgaga
     9241 atggatataa aatatctatg acaatactac atggaaagac aatctgccat gtagtatctc
     9301 ctgaaacaat cttaggaaga aagttcacat tagaagaaaa acaagaaaaa gatacaataa
     9361 tatatggagc aatagaaaaa ataatggtag acagacaatt acaaataaaa aattcaaaca
     9421 aagaagttgc tagtcattag cacttcttta ataagtaaag taggacaagt attttttata
     9481 atattattga cttatagtct aattagacta taatataaac atagatagtc taattagact
     9541 actattaaaa ataaatacaa taagaattta agaaaggata ataataataa tgattaaatc
     9601 atttttaatg ttaggacagt ccaatatggc tggaagagga ttcataagtg aagtgactcc
     9661 aatttataat gaaagaatac agatgttacg taatggaaga tggcaaatga tgacagaacc
     9721 tattaattat gacagaccag tttctggggt aagtcttgct gcatcatttg cagatgcatg
     9781 gtgctgtgaa aatcaagagg atagaattgg gctaattcct tgtgctgagg gaggcagttc
     9841 cttagatgaa tggaatatag atggtatact ttttaaacat gctataagtg aagctaaatt
     9901 tgccatacaa agcagtgaat tgacaggaat tttatggcat cagggagaaa atgacagtaa
     9961 taacggcaac tataaatttt attataaaaa attactttct attattgaaa ctcttagaaa
    10021 agaattaaat attccagata taccaattat tattggtgga ttgggagatt tcttaggaaa
    10081 agtaggtttt ggaaaaagtt gtactgagta tgtatttatc aatcaagagt tacagaaatt
    10141 tgcttttgaa caagataatt gttactttgt tacagcaaca ggtcttacat ctaatcctga
    10201 tggtattcat attgatgcaa tatctcaaag aaaatttgga ttacgttatt ttgaagcttt
    10261 tcataagaaa aagcatatta tggaagcatt agctaatgaa agtgagttga taataccgag
    10321 taattccaag acatatacaa aaacagaaaa aatatatata aatagtatgg acttggcact
    10381 aggaaagata tcatatgatg agtttgaatc taaacttatt aaacttaatg ataattagat
    10441 aaaatattca ttaatatact aataaatata gattgaaaga ggtgattttt tgataccatt
    10501 attaatttta ggattattaa aacaaagtcc aaattcttat ggatatgagt tattagcatt
    10561 aatggaaaag aagcattaca agtacatagt aaatttcact aaaggttctt tttattataa
    10621 tttacagcaa cttgaagaaa aaaagtttat tgaaaaagtt gaaaataaac aatctaataa
    10681 aaacagagaa actcataact acataattac tgaacttgga tgccaggaat ttgaaaaatt
    10741 aatgtataag tatggttcta aaacagagta tataaatcta tcattttacg caacactatt
    10801 atttgcaaat gattatgaaa atgaaaagtt aatagaatta ttggaaacgc aaattaagca
    10861 aactcaagta agaatttctc tattaaatga atccatagaa aatgacaaaa caaattcaca
    10921 ttattttaat agaatgctag aaaattctcg ttctcaccat ttagttaata ttgaatggtt
    10981 tgagaagttg ctaaatgata ttagagattg attttaataa taaaaaaaca tacatatata
    11041 ctttatataa atgtatgata tatagattaa aaataaggca gataattttc aattgaatat
    11101 ctgtcttttt gttatataag atgttaaaag acacttatac tatgtaagta aaatttataa
    11161 atataaagtt gttcattgtt gaattacaga cacttaagat gaaaagtatg acatttgata
    11221 taataaaatt gtttaagtat taattatgag tattaattaa tggtatagat aattttaata
    11281 taactgtatt cataaataat tataaaataa taaattaatt tttttatact gactttttta
    11341 tagtataaat cgttatatat aatgacggag gtgataatag tgaatgatgc tcggattata
    11401 tcacaaataa aagcaggaaa aaaagaattg tttgatactc taataaaaaa atactatgat
    11461 agcatctact gttattgtta tcgccatgta gacaataaaa tgactgcaca agatttaact
    11521 caagatgtat ttgtaacagt tataaataat atagaaaatt ataaacatta tggtaagttt
    11581 gaaaactatt tatatgttat tgcaaaaaac aagtgtaagg atttttggaa gaaaaaacaa
    11641 ccaatatatt tagagtcatt agataaggag atatcaaatc aagaattatc tgatatagaa
    11701 aataaaatgt ttataaaaga agctttgact aaattaccag aattagaatt tgaagtgata
    11761 attttacggt attaccatga tttaagattt aaggatattg ctaaaattat ggagtcaagc
    11821 gtttctgtca caaagtacag gatgagtagg gcattaaaaa aactagaatt atatctggaa
    11881 ggaggtaaat tatgaataat aaagaaataa aaagaagtct acagaagtat aaggttccca
    11941 aatatgattc ttcagctctt gaaaagacta ttaaaactat acataagtta acgcttagta
    12001 atacttataa aagaatgaat aataaagact tttttatatc tcaaattggt tttataagaa
    12061 aaagaacttg gattatgcaa tttctaatac tatgtacaat atgtatattt ttatttagta
    12121 catcagcaga tagtaattca ttttataata ctttatcctt gatttctata gcatcaccac
    12181 tttttgtact gacaaatata gaagaattaa caaaagtata taatagtagt attttagaga
    12241 tagaattttc aacaaaaaat acattacata aaattattgc tactagaatg cttatatttg
    12301 gaataagtga tttattgttt atgattatta ttttggtatc tgcaaaacaa atcattgatg
    12361 taaacattct gtatataata atttatatac tagttccttt taacctaatg tgtataggat
    12421 gtatggaaat tataaataga aataaaggta agcatacaaa ttattattgt atgatatatg
    12481 gtatatctct tgtattatta atagttatac tgagtaactt tagagataat ctatatcaat
    12541 ttgaagcaat aaatcaatgg ctaattgttt ttctagtcac agcagtaaca ttaatatatg
    12601 aaataagaaa aacgtggaga catctttgtt catttgaaaa tattattatc taagtaaaaa
    12661 ataagaatac acagtatcag gaggaatagt atggagttaa gtgtagaacg tttaacaaaa
    12721 cagtataaca ataaaattat agtagataaa attgacttta aattaaatga gggtatatat
    12781 ggatttttag gagaaaatgg agcgggaaaa acaactctta tgagaatgat ttgcggaata
    12841 ttaagtccta catctggaga agtaaaggtg gatggtaaaa ataatttgga tatgggagaa
    12901 gattttagag atatgattgg ataccttcct caaaactttg gatactaccc agattttact
    12961 gccaaagaat ttatgctata tatagcatca ttaaaaggat taatgcctag ctatgcaaag
    13021 agaaaaacac aagaattatt agaaatggta ggattatcag aggtaattaa taaaaaaata
    13081 agaacatttt ctggaggtat gaagcaaagg ctaggatttg ctcaaacact acttaatgac
    13141 cccaaaattt taatattaga tgagccaaca gcagggctag accctaaaga acgagttaga
    13201 tttcgcaacc taatttcaga attttctaag gaaaagattg ttatattgtc tactcatatt
    13261 gtgtctgatg tagaatatat tgcagataaa attttaataa tgaaaaaagg aagatttgtt
    13321 ttaactggct cagtagaaga agtaacttca aagataaaag ataaggtatg gaaatgtgtg
    13381 gtcaatgaga atgaaataga ttatatgtct gatagatatt gtttagcaaa tatccatcat
    13441 tctggtggag ttgcaaatct tagaattgta agtgatataa agccacatga caatgcatgc
    13501 aatttaaaac caacattaga agatttatat ttatattgtt tcaacgaagt actataaata
    13561 tgcataagga gataggtatg tctacattaa ttaaatttga gttaaaaaaa ataataaaaa
    13621 gaaagtctac aatagcagtt attttaggaa gtttattctt aagcatcatt ctttttgcat
    13681 taatgccaat tcaggaaaaa gctaatgatg aaagtggaaa agagtatagg ggtttacagg
    13741 ctattgagct aaaaaagcaa caatctaaat taaatgaggg atacataaca gataaaaaag
    13801 taaaaaactc aatcagagat taccaaaaaa tgtttaataa tccaaataac attgtaacaa
    13861 atgagtctgg attctcagat tttaagccag atatttattg gaaattcttc tatccaaata
    13921 aaacttttta taattttata atagagtcat atagtaaacc aaatgatttt aacccagaag
    13981 taattaaatc aatgccatta gaaaatgaaa ttaattttta taaatttaga aatgaaaaag
    14041 taaatacaat attaaatatg gaatatccag agggaaatta cacacagaaa gaaaaggact
    14101 tttggttatc taaaaataag gatataaagg agccttattt aaatggatat tatcaagggt
    14161 gggaatattt tctattggca agagatttac tgatttttac tataatgtct gtctgtataa
    14221 gtatagcttc tgtattttca ggagaatacc aatctggtgc tgattcagta gtattgactt
    14281 ctaaatatgg aaaaacaaaa gttataacag ctaaaatagt aagttccttt ttatttggta
    14341 cgattatttt tacaataaat acaattgtgt ctattggtat acttctttta tcttttggaa
    14401 ttgatggatg gaatttacca ttacaactta caaccagttc aattccatac ccatatacat
    14461 ttttacaggc aatattacta agtgtgttaa ttatttatat gatattaata ctattaatta
    14521 gttttacact tttattatca tcttatatga aatcaccttt tccagtttta attacaaata
    14581 taatattact atttgtacca atatttttaa aatatagtga aacaaataag ttgtataatc
    14641 aaattctcaa tgttttacca tcaaatgcta tttcattttc atttactaat tatgtagatt
    14701 ataaatttgg taatttaata atagctttac ctacaatgat tatttttaca tgcttagttc
    14761 ttacagttgt ttttttacct tttattaaaa ataattttaa aaaacatcaa gttaactaaa
    14821 atatatatta taaaaagaag gtgtcttaaa atgaacttct tagcttatta ggcactcttc
    14881 tttgtattaa tttaaatata ttttagtatt agattaaata tgttttcatc attttaacaa
    14941 tgaaaaagag ctttatctct attttgagat agctcttata aagttaaata ttaaattgat
    15001 aactataaaa tatgtagttt gaatttataa tttgtgttat aatcatactg atttttgtac
    15061 atcagttctt acattaacat tgcctgcttt tagattttct ccaatagtaa gacctttttc
    15121 ttttagaaca tattcaaaag tcattagtgg aactccacct tttctaccac ctaatatttc
    15181 tgttcctttt aaatcattgt aagagaaatt aggcattttt ttcctagcaa ctagaaaact
    15241 accatctctt tttgtcaatt gagcaaagtt cactgcataa tcgtttttgt tttgattata
    15301 tacatatata gatgcttcag gacccattaa acctacgttt gcttcacctg aaatcaaagc
    15361 tgccatagtt ttatctgcac cttgggtatt tataaggtca atctttatac cttcatcttc
    15421 aaagaatcct ttcgtaatag cagcatactg tggagcataa aaaacagagt gagtaacttc
    15481 tgctatagtt actttatcca gatttttaga cgaagaagat tcatcttttt tgtcattaga
    15541 gttaaaatca catccagtta gacatattgt acttacaatg atagagcttg ctattgcgac
    15601 tattttttta aacataataa atacctccca taagacatac gaatagtata tagtattaaa
    15661 aatttattgt agttgttaca aaaatgtatc tatgcttata atatttctac aaaaacttaa
    15721 taataatttt cagttttatg tttagt
//
LOCUS       DAFKMX010000096        15700 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFKMX010000096 DAFKMX010000000
VERSION     DAFKMX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.096"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            338..1414
                     /locus_tag="LZB08_002298"
     CDS             338..1414
                     /locus_tag="LZB08_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBM7315795.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1517..2359)
                     /locus_tag="LZB08_002299"
     CDS             complement(1517..2359)
                     /locus_tag="LZB08_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBM7315796.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2543..3115
                     /locus_tag="LZB08_002300"
     CDS             2543..3115
                     /locus_tag="LZB08_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7315797.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3278..3550)
                     /locus_tag="LZB08_002301"
     CDS             complement(3278..3550)
                     /locus_tag="LZB08_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315798.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3664..4536)
                     /locus_tag="LZB08_002302"
     CDS             complement(3664..4536)
                     /locus_tag="LZB08_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7315799.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4832..7939)
                     /locus_tag="LZB08_002303"
     CDS             complement(4832..7939)
                     /locus_tag="LZB08_002303"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBM7315800.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVEALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(8032..8264)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8439..8966)
                     /locus_tag="LZB08_002304"
     CDS             complement(8439..8966)
                     /locus_tag="LZB08_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBM7315801.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(9001..9786)
                     /locus_tag="LZB08_002305"
     CDS             complement(9001..9786)
                     /locus_tag="LZB08_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBM7315802.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9796..10053)
                     /locus_tag="LZB08_002306"
     CDS             complement(9796..10053)
                     /locus_tag="LZB08_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBM7315803.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(10072..10530)
                     /locus_tag="LZB08_002307"
     CDS             complement(10072..10530)
                     /locus_tag="LZB08_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBM7315804.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10570..11274)
                     /locus_tag="LZB08_002308"
     CDS             complement(10570..11274)
                     /locus_tag="LZB08_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBM7315805.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11284..11919)
                     /locus_tag="LZB08_002309"
     CDS             complement(11284..11919)
                     /locus_tag="LZB08_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315806.1"
                     /translation="MKKVKYTRLVILGILIYLVFNTLVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12300..13340
                     /locus_tag="LZB08_002310"
     CDS             12300..13340
                     /locus_tag="LZB08_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315807.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13803..15158)
                     /locus_tag="LZB08_002311"
     CDS             complement(13803..15158)
                     /locus_tag="LZB08_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBM7315808.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDETDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15184..15453)
                     /locus_tag="LZB08_002312"
     CDS             complement(15184..15453)
                     /locus_tag="LZB08_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBM7315809.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         5008 a         2713 c         1805 g         6174 t
ORIGIN      
        1 aaatgataaa tttaaaagtt ttaccacttt aattctaatg atactaaatt tttaaaattg
       61 tttcaatatt attttattat aaaattttat ttattatttt tcattagtat ataaaaagat
      121 aaaatacata actttataga cttaatttat tacttttata gaattttcac aataaaaagt
      181 cattttactt ccgtatttaa tgcaaaagta gagattaaac ttaatataaa aaatatataa
      241 caaatttttt ttatatttct agaagaaaag tatactcttt tttgtttaat aatgctaaaa
      301 ttgatttgta ttgaattttg attaggagga aattattatg aaagctcaaa gattaaatgc
      361 tgtacttgaa caaatgaaaa aagatgatat ttctcaaatg ttagtaagtg accctacttc
      421 tatattttat cttacaggag tactaatcca tccaggcgaa agactattag cattatactt
      481 aaatttaaat ggcaataata aattatttat aaatgaatta ttcccagttt cagaagattt
      541 aggtgttgaa atggtttggt ttaatgatac tcaaaatcca gtagaaataa taactgaaca
      601 tatagacaaa aatgctacta tgggtgttga taaaaactgg ccagctagat tcttattaaa
      661 cttaatagaa ttaggtggag gaagtaagtt tgtaaattca tcttatataa tagatacttt
      721 aagaatgtgt aaagatgaag aagaaaaaga gttaatgaga atagcttcaa aattaaatga
      781 caaggctatg gaacaactta aagctactgt ttctggtgaa cttacagaaa aacaacttgt
      841 tggtaaatta tctaaaatat atgaagacct aggtactgat ggattttcat ttgaccctat
      901 tataggtttt ggacctaatg gagctaatcc acatggtgaa cctggaaatg cacttgtgaa
      961 accaggagat gcaataatct tagatatagg ttgtataaaa gataactatt gtgctgatat
     1021 gacaagaact gtattctata aagaaatacc tgaaaaaggt agagaaatat ttgaaatagt
     1081 tcttgaagct aataagagag ctgaagctat tgtaaaacca ggtgttagat tctgtgatat
     1141 agatgctgct gctcgtgact acataactga aaagggatat ggacagtact tcactcatag
     1201 aactggtcac tctataggac ttgaagttca tgataaagga gatgtatctt ctataaatac
     1261 agatactgta caaccaggaa tgatattctc tatagagcca ggtatatatt taccaggtga
     1321 gttcggagtt agaatagaag atttagtact tgttactgaa gatggttgtg agatattaaa
     1381 caagcatgac aaagaaatat gtgtagttgg ttaattctaa aatcaatttg ataaaattaa
     1441 aaagccatat aaaaatgttt taaacatttt tatatggctt taatttctca atctatgatt
     1501 tatttcaatc cattatttat attgcagtta ttaagattgc accctttgaa gtatatgtcc
     1561 ccatcactga accaatttca cagattacaa catctttgta ttttacatcc ttttccataa
     1621 cttcccttac cttttgagcc ttttctgggc aattagcatg tcctataaat aaaacctttt
     1681 cttctttatc tttaattttt gactctacta aagtaatcag ctttttcaaa ctgtttcctt
     1741 cacctcttgc cttatctata ggagttacaa cgccatctgc aacttgtatt atcgccttaa
     1801 aatttaaggt atttataatt tttcctgcta ttggatttat tctaccaccc ttaatagcat
     1861 tttctaaagt ttcaagtgtt ccaaaaaatg gaggattatc tttatattta tttattattg
     1921 aaacaatctc ttcaatttct tttccttctt ttattaatat tgctgctttt aacactaatt
     1981 gtcctattcc aattgaccca ctaagtgaat ctataacctc aactttcttg ttatttcctt
     2041 ccttttcaaa catatctttt gctaaaagtg cactattata agtcccagat aatcctgaac
     2101 ttatattaat cactaataca ctttcttcct gacaatggta tgcttctaaa aatttatcag
     2161 gagaaggaca tgatgttttt ggtagtactt tactctcact catttttcta tagaaagttt
     2221 cattatctat ttcctttcca gatatataac tttcttctcc aaaagataca tttaaaccaa
     2281 caacttctat gttgtatttt tctattatat catcaggtaa gtcacatgca ctgtccacta
     2341 tcaattttat tttatccata ttaacctcac tataagatat tattttattt attcagtcgg
     2401 tcgtacgacc gatttaatta ttattttata tattgaatga aaaaaagtca atagatatag
     2461 attctcgctt tattgacaat aaatatccat aagcttataa tgttattaat ttgttataaa
     2521 tatacatgga aaggtaggtt ttatgaaaac aaataatatg aaacaggcta ttttatcatc
     2581 tgcaaccaat ttaatagcca aaaatggagt tcaaaatact agccttgctg atattgcaaa
     2641 agatgtaaat ataagtaaag gtactttgta ttatcactac gcatctaaag atgatattat
     2701 atatgatatt gcggatactc atttagaagt tataactagt gctatactaa actgtgtaaa
     2761 aaatgtcaaa tcaaaaaatt ctcaaataga aatggttaac ttaatcttag agaaaatatc
     2821 tactatagaa agtcgtggta gggtacatat gtaccttatt tgtgaggcaa ttacttcaaa
     2881 taatgattta aaggaaagaa taagattaaa atatattgaa tggagaacta ctcttaaatc
     2941 tgaaattgtt gaatcactag aaaaatataa tagtagcgaa aatgaacagg atgctgaatc
     3001 attttctttt ttactcatgt caatagttga tggtttagtt gtccaaagtt tattaaaaac
     3061 agaaaaaata ccttatgaaa atatagcttc ttttttagtt aatcattgga tttaaactta
     3121 atacttagat aattaataaa agtgaactat ttaagttatc tactttaaca gttcactttc
     3181 ttatttattc tcaataattt ttacttaaaa cattcaattt ttatttaaac cttcatggaa
     3241 tcacaaaata aaaatctctt ataaatttaa aatgttttta tttatgttca tgtgtatgcc
     3301 catctgaatg ttcatgacca tgtgtatgtg tatgctcatg gctatgctca tgagtacctg
     3361 aaaaaccatc atcatgatta tggtcgtgac ctacaccatt tacatgctca tgttcatgcc
     3421 catgactgtg agaatgttga tgtgaatgtt catggttatc tcctccacag catccttctc
     3481 ctccagtccc acaacaccca cttgtatgtg gattatgatg atttcttact acaaatctct
     3541 ctcttaacat aattcttata cctcctagta tattattgag tacttcatgt accccattaa
     3601 ctacagtata catctaacta ggtataatct caatacatat ggcatttttt aacatatata
     3661 atcttaaaga aatttatcct taaaacctcc accttttact tcttcaacgt tattgacaga
     3721 aataaaagct tcactatcca tatcataaat taaatcttta aatttaccta tttcatttgc
     3781 agatattata caataaatca tctttttctt attttgtgta tatgcacctt ctgcctccaa
     3841 aaaagtgact cctctaccca ttttattcat tatcactttg gatatttcat catatttatt
     3901 tgatattaaa agtatagatt tttttctatc aaagcaatct tttcctatat ccatagtaaa
     3961 agaaattata tacattgtaa ttaaagtgta catcaccaat ttagctccaa ataaaaaagc
     4021 tcctgcacat actacaaaaa aattaataaa taaaaatgta tttttcatag gtatatcata
     4081 ctttcttttc attatggctg ctattatatc aagtcctcca gcagtagctc ttgctttaaa
     4141 agttaacccc atacctatac cagttaatgc accaccataa atacactgta gcatagtatc
     4201 atctattggg aaatatttag caatatcttg tgttagcccc agtgctagag aaaatagtaa
     4261 catattgaca aaacttatta gcaaaaattt attatcaaaa tactttctag ctataataaa
     4321 tattggtatg ttcatcaaga aacttgatat tccctgattt ataccaaata atttgtataa
     4381 tataacacaa attccagcta ctcctccact taataacttg gctggtgcta gatataaatt
     4441 tattcctaag gttgatagaa tcatacccaa tataatcaga caatattgta aaaatatatt
     4501 attatcaata cttagctttt tactttcacc atacatcaaa acgcacccct ttgcataata
     4561 aataaaccac cctgcataac acacgataaa tttacaaata aatttatcag aagtagtttc
     4621 ctaaaaatat tattcatcct cacttaaacg tttattcatt aatattctat cacatgacat
     4681 aaataattta tatgatttat atagatttat ttattgtaaa ttttggaata ttaaaacatt
     4741 ttattgaata gataattata ttacataaaa aatccctata gaaatctata aactaatttc
     4801 tatagggatt ttggttaact aactattatt tttatttttt tataactttt atacctgtca
     4861 tatggtcatt taagttttgt tcctctaaag atttatcatc tactctgttt atttctaaag
     4921 ctaaagtttc agacttgata tattcattaa aatggtcaac agccttagca atttcatcat
     4981 caccacagta ttctattact atattatcta atacttcaaa gttattagat tttcttaatt
     5041 gctgtacttt agatataaat tctctagcat aaccttcatt aatcaactct tcagttagct
     5101 tagtatctag tataacaaaa agattgtttt cgacagatac attaaatcct tccttagcag
     5161 ttatacctat taatactaaa tccttattaa attcaaaagc ttcaccatct aaatcaactg
     5221 ttaaagtttc acctttttca agtttattag cagtttcatg agcatttaat ttacttaatg
     5281 cttcaacgaa taaattcatc ttagaaccta ataatggccc tgcttcctta aagtttggtt
     5341 ttaaactaaa gttcatatac tcatctaaat ccttagcaaa tattacctct ttaacattaa
     5401 gctcttcttt tattaaatca actacatcac ttattgtatc ttcaaactta ccatctacta
     5461 acactttttg taatggttga cgaactttta ttctttctac ttctcttgaa gctctaccta
     5521 aagtaactaa gtttttagct aaatccattt tttcttctaa tttagtatct actaactctt
     5581 cattacattt aggatattca gctaaatgaa cagatacttc tcctgttaag tttctataca
     5641 tttcttctga catatatggt gcaaatggag ctatagctct acaaagacca tgtaacaatt
     5701 cataagttgt attgtacact gcttttttat cttctgttaa ttcagttgcc cagaatcttc
     5761 ttcttgaacg tcttatatac cagttagata aatcatcatt tataaaatct tgtatcattc
     5821 ttaaagtttt atttaactca aatatagcta aattttcttc tacatctttc attaaattat
     5881 tgaacttaga taatatccat ctatcaagtt ctggtctatc tttatattct acaaaaaatt
     5941 cagttggatt tatatcatct gtgtttgcat ataaagtaaa gaagttatat acattcttca
     6001 tagtacctat aaatttactt tggatttctt ttaatccatc catatcaaat ttagttggag
     6061 tccatggtgg agaaacatat agtaaatacc atcttaatgc atctgctcca tattggtcaa
     6121 ataattccat tggatttact gtatttcctc ttgatttact cattttctta ccatctttat
     6181 caagtacaag atctggaact aaaacactct tatatggtgc ttttcctaat acaaatgttg
     6241 atactgcaag taatgagtag aaccaacctc tagtctggtc tataccttct gatatatagt
     6301 cagctgggaa caattggtca aagttttcct tattttcaaa tggatagtgg tgttgagcaa
     6361 atggcatagc cccactatca aaccaacagt ctataacctc tgttactctt gtcattggtt
     6421 ttccacattt ttcacatttt aagtgtatat catctacata tggtctatga agttcaactg
     6481 tttctggatt tacatcttct atagcttttt cagctaattc agctcttgaa ccaactgaat
     6541 ctgtatgacc acattcacat ctccatatag gaagtggagt tccccaatat ctagttcttg
     6601 atattgccca gtcatttaga ttttctaacc agtttccaaa tctcttctct cctacaaaac
     6661 ttgggaacca ttttacacca ttattatttt ctattaattt atctttaagt ttagtcatct
     6721 ctatatacca gcttggtttt gcatagtata ctaatggagt ttgacatctc cagcagtgtg
     6781 gataattatg aactactttt tctttgctat ataatttatt ttcagaagct agccatttta
     6841 ttatttcaac atctagccca tcttccatta caaatctacc ttcccaagga gtagatgtaa
     6901 atttaccagt ttcatctact ggttgaataa ctggtaaatc atatctttta cctgtgttgt
     6961 agtcatcttc accaaatgct ggtgctgtat gaactatacc agttccatct tcagtagtta
     7021 cataatctcc tactgtaata aaaaatgctt ttttgtcaac ttctacaaaa ggcattaatt
     7081 gttcatattc taaaccttct aggtctttac ctttcatttc ttctaaaact tcataatctt
     7141 cacctagtac tttatcagct aaaggtttag atacataata tacctcatca ccttttttaa
     7201 cttttaaata atctacatca gcatttactg ttaatgaaac gtttgatggt aaagtccatg
     7261 gagttgttgt ccatgctaag aagtattcat cagcatcttt tttcttaaat ttagctataa
     7321 ctgtattagt ttttacttct ttatatcctt gtgcaacctc atgactagca agaccagttc
     7381 cacatcttgg gcaataagga agtattttat gtccttcata tatatatcct tctttgttga
     7441 atttgtttag tatccaccat acagactcta tatagttatt atctaaagtt atatatggat
     7501 tatctaaatc aacttcataa gccattctct cagtcattat tctccattgt ttttcaaatg
     7561 agaaaactga ctctctacat ttttggttaa attcagctat tccatattct tctatttgtt
     7621 gcttactagt aagacctaac tctttctcta cttgtatttc aactggtaat ccatgtgtat
     7681 cccaaccagc ttttctcttt acttgataac cagacattgt tttatatcta caaactgaat
     7741 ctttaagcgt tctagataaa acatggtgta ctccaggatt tccattagct gttggaggtc
     7801 cttcatagaa tacgaaactt gggtcatctt tccctttttc taaagttttt tcaagtatat
     7861 ttatgtcttt ccaataatca aaaacttgtg cttctgcttg ttttacagaa ctgtctacca
     7921 atggcttaaa tttcgccatg taaatcaccc tttcctacta aatattataa ttccaaaatt
     7981 ttttattctt tcacataaat ttaaatacta aaaaactcgc cccctttaaa ccaaagggac
     8041 gagttataaa tcgcgttacc accctaattc tatatgtaaa ctaaatacta gctacatata
     8101 acactctaat agtataacgg tactaaccgg cacagcttag tagaatttaa tcgttcagcc
     8161 tgcagctcaa gagtgatttt tatctttata aattcattgg ttctcagcat acccaattct
     8221 ctgtaaaatt atctaaagat aaatgtctct atcaacgctt ttaaaaattt atctgtgttt
     8281 atatgaaatt ctataacaaa taatttgaat agtcaatata tttttgaaaa atataataat
     8341 ttctattttt cttacatttt tatcctattt ttaatctttg ttatagattt ctcaaactca
     8401 cacaattatt ctacataaaa aataaacaaa ttacaagctt attctaaagt tgtagcagct
     8461 tcatcattac tataattata cactgttgtt ccttcaaatg catttacaca tttttcatct
     8521 acatcataaa aaatctcatc aacacttctt atttcatctt ctaacagtga tttaaattta
     8581 tttctaaata tcttaaattc tttgactaaa gcatcatact cttttcttat ctctataaca
     8641 cggttattag cttgttctat aatttgtcta gcttttaaat cagcttcttc aacaattatc
     8701 ttagcctttt tattagccgc actacaagtg tcttctgctg cactttgagc agttattaaa
     8761 gtggctttta gtgtttcttc aatattttca tacttgttta cttggtcttg atataaccct
     8821 aatttttctt taagagctgt attttcccta catagagatt cataatcttc cttgatgata
     8881 tctaaaaatt catcaacctc ttcatctcta taccctctta aacctttttt aaactcttta
     8941 ttttctatct caattggagt tagcattaca tttcctccca gctataattt aaattagtcc
     9001 ttatacaatt aatcttgctt gaacttttat ttttcctttt ttagtaatat ctcctatttg
     9061 tgttacctta gctcttcctt tgcctctaat agaaatcaaa tcatcatttt ttactatttt
     9121 agaagttgaa gttatctttt cataattgac atggactttt tctccattta tgtattttgc
     9181 actatcttgt ctagatatat tgtaaatacc acttatcaca caatctaacc tatctgaaga
     9241 tactgtaaat gatacttctt tatattttga agaactatta acaatatttt ctctggaaat
     9301 ttcctttaca attacattat ttcttgaaac cttttctaag tttacaatta taaaatcgca
     9361 aatatcacta cttacaacta cttgacaaaa gctatcatgt ataattatat ctccaatttt
     9421 ttctcttttt atacctagac ccagtattga ccctaagtaa tctttatgag atatattttt
     9481 aaatttgaaa ttcccttcta tttgtaaaaa tctaagagta tcttctatat cttcatattc
     9541 catgtaaaat ggataaataa atacagtgct tctttcagct cgttcataac ctccatccac
     9601 actgtatttt atttcatttg tagaatttaa tattgctaca gcatttttca cttcatatgg
     9661 attaagaaac tccgtagact taacatcata gttttttata caactattag ccttgtctat
     9721 cactttaaac attttatttt tcaagtctat atcctttatg tgatttgtta gttttaattt
     9781 atccatccta tcaccttaga atataaccaa tactaattgt cttaggaaca tcaataatac
     9841 tattgcaatt aaaggagaaa aatctatagg tccatttgta tatttataca ttatagacct
     9901 tattggccct tctattggct ctgtaagatt ttccaaaata tcaaaaattt tactttgtct
     9961 tccatttgga aaccaagtca tcaagctttt tataactatc atccatgcta ttatatctaa
    10021 aagataataa agtgcaattc ttattgttcc catatattaa ccgccttttt actatttatt
    10081 ttgccaaggg aagaatgctt tactttctaa ttcttttttc atatttgcat ctatatccac
    10141 attattagga gctagtataa atattgattt agaaaccttt tgtatacttc catctaagac
    10201 ataaactgcc ccattcataa agttaaatat agatttacga acttcagaat tagtcaatcc
    10261 ttccaaattc actataactg cccttctttg ttttagatgg tctgctatag ttgcagcttc
    10321 atcataaacc ttaggttcaa ctattacaac tttcatttgg ctagttgtat gtaaatttac
    10381 aattttattt gcttttgcag tactaaagcc actgttcatt tcttcttctt gaactatatc
    10441 gtccatgcca ctttcatatt catcttcaat gtaatcttct tcttcatcaa ctatccaatt
    10501 cttaaattta gatattattc catttgacat tattaacttc ctccttaaaa tttattcaaa
    10561 tttgagtaat tattttttat tgtagtttct ttctccaaag attgatgttc caactctaac
    10621 aatggtagcc ccttcctcta tagcaatttc ataatcgtga ctcattccca ttgacaaaac
    10681 atccatccct acattaggga tgtttagttc ttttatttgt tttgataaat ctttaagacc
    10741 tttaaataca cctcttatct catcttcatc ttctataaaa ggtgccattg tcattagacc
    10801 ctttattttt atatttttat atttttctga cacttctttg acaaagtcta caacttcttc
    10861 tctatcaagt ccatgcttag actcttcttt agatatattc acttgtacta agcaatctat
    10921 ttctctgttt atttgttctg cacgtttttg aatttcttcg cataatgcaa ttctatctaa
    10981 agagtgtatc atacaaactt tatcgattat gtacttaact ttattggttt gcaaagttcc
    11041 tatcaggtgc cagtttaatt tgtcacctat aatatcatac tttctagcta attcttgggg
    11101 tttattttca ccaacagaag tgattccttc cctcattgcc tcattaacta agtctacatc
    11161 aacagtcttt gtgactgcca aaagggtaat ctctttgctg tctctatcta ctttttttgc
    11221 agctttatca attttgtttc ttatctcaga caaattatct ttaatagaac ccattattat
    11281 cacctacttt atccttatat ttgtgtttat actgttaggt cttaaaatta ttttgtcata
    11341 tagattaaca cttgtaactc catttatgtc atttttataa taatccacaa atacagaaga
    11401 ttcattctca taagttattc cttctaatat gacaaatttt acactttgtg attgttcatc
    11461 aattacatat acaccacgtt tattgtcttt tgtttttata gaagttttag gtatttttaa
    11521 acattccatc tgattatata ttatatcaaa ttcttcgaca cttgtatcct gatttgcaac
    11581 aatttggtta cttattttta attttaccat tattttatta ttattttcat atattttgta
    11641 aacttttgca tctaattttt gctcatgata ttcaattatt acattttgat ttttttcaaa
    11701 gttattagaa tcttctttac tgatgtatgt agatagataa ttactctggt cattggcatc
    11761 tttttctata gttcctttta catgaatctt tgcatcaact ttttcatttt ttaacaccaa
    11821 cgtatctaca tttttgctaa ttatcattac taatgtatta aatactaaat atataagaat
    11881 accaagtatc actaatctgg tatattttac ttttttcaaa gactctcccc ctataaaatt
    11941 actttcatta taagtacttc ttctaaaaaa tgtttattcc ttttttataa gctaatattt
    12001 tacgttctaa taattatttt ttccatatta aatttatttt ttatcaatct tcctatacaa
    12061 gactgcatta tttatatata attgtattat aaagtataag tttaatttac attttatacg
    12121 ttaagcttac tttatatgtt tttatagtta tttttatttt tagatacaat ataaaaccaa
    12181 ttaaaattta aaataacttt tgttatgttg acatttattt aggtaatcat ttacgaatca
    12241 ggataacgtt atcataacaa aagtttatgt cctaaaaaaa atacaaggag gattttctta
    12301 tgtcagaaat tttattagga acaggtttac ttttattggt tctatgttgt ttcacaattt
    12361 ttagttacaa ggccccatat ggtatgaagg ctatgggagc tctagcaaac gctgcatgtg
    12421 ctactttctt agtagaggca ttcaaccttt ctttacttgg tgatgtttta ggtatggaat
    12481 tttttagaag tgttggagct gctaatggta gtcttggtgg agttgctgct gctatacttg
    12541 tgcctttagc cttaggtgta tctccagttt atgcagtttt aataggatta tcttgtttag
    12601 atttttctat attatctgga ttcgttgctg gatactttgt ttctttctta attaagttta
    12661 tagagaaaaa agtgccacct ggagtggatt tagtggttgt aattcttcta tgtgcaccac
    12721 tatctcgtct tatagcatca aatgtagacc cattagttaa tagtacacta ctacaaattg
    12781 gacaagtttt gatagaagct accaacatta gccctataat aatgggaatt atactaggag
    12841 gtctaataac agtagttgga acgtctccac ttagttcaat ggctcttaca tctataattg
    12901 gtcttactgg tcttcctatg gcaataggag cattaggtgc ttttggttca gcttttatga
    12961 actcaacact atttgcaaaa ttgaaattag gttctaaaaa agatattata tctgttgcta
    13021 ttgaaccatt aacccaatca gacataattt cagctaatcc aatacctata tattctacta
    13081 atttctttgg aggagccttt tctggtatca ttgtagcctt gatgggtctt gtaaacatga
    13141 caccaggtac agctacacct gtggctggtt ttgctataat gtttgcttat aatccagttc
    13201 aacaagttct tataacagct ggtttatgtg ctttattcaa tgtaattgca ggatttttag
    13261 gttcaaaaat attcagtacc tttaaagttc ttactgctgg tgaaatacgt ggtactgatg
    13321 tagaagaaga aattgcttaa aaaacaataa gatttataaa tttatatctc aagtagtaga
    13381 gttttatatt gtaggcaatt ttattaattg caaacgtttt attttttatt tatgtttatg
    13441 attattatgt gtgttgaata tttagataaa tttttattta ttatttttat ttagattttt
    13501 gtttagtttt ttatttggtt gatatattag gaataaaaat tagtatagtt tattactagt
    13561 tagatagcat aactttttag atacataaaa aattttatat taattagata ttattaaaat
    13621 aaaagcgata catgaatttt taaatgtcca tgtatcgctt tatttttgcc ataatcttaa
    13681 caactgattt ttaaagttta ttatcttaac aaagttcatt atcttatata atatttaaat
    13741 gtatagactt ctttaattta accatctaaa tatttaatta aatattatta tatgtaatat
    13801 aactaatttt taaatgaatg tataggagct ggaatccttc ctcctctttg agtaaataat
    13861 tcagatgaat ttgtatttat aggcattact ggtgctcgtc ctaaaagtcc tccaaattct
    13921 accatatcac ctacatcaca tcctggggct ggtataattc taacggctgt agtcttatta
    13981 ttaatcatac ctattgctgc ctcatcagct atcatggctc caagcgtacc tgctgtagta
    14041 tttcctggca cagctatcat atcaagacct acagaacaaa tagcagtcat tgcttcaagc
    14101 ttatctatac ttaaagcacc tttaataaca gcatcaatca tacctgcatc ttcagatact
    14161 ggtataaacg ctccactaag tcctccaaca tgtgaacatg ccattactcc acctttttta
    14221 acagcatcat ttaaaagtgc taatgctgct gtagtcccat gagtaccaac aacttcaaga
    14281 cccatttcct ctaatatatt tgctaccgaa tcaccaatag caggtgtagg agctagtgat
    14341 aagtctatta tcccaaatgg aacatccaat ctactagaag cttcctttgc cacaagttgt
    14401 ccaactcttg taattttaaa tgcagtcttt ttaactgttt ctgaaacaac atcaaaaggc
    14461 tctcccttta ctttctctaa agctctctta acaacaccag ggccacttac accaacattt
    14521 ataattctat cagcctcacc aacaccatgg aaagctcctg ccataaatgg attatcttct
    14581 actgcattac aaaatactac taattttgca caaccaaaac cttttgcatc ttttgttaat
    14641 tctgcagttt cttttatgat ttctcccatt tctttcactg catccatatt tataccacat
    14701 cttgtagaac ctacatttac tgaagcacat actttattag tacttgctag agcttttgga
    14761 attgacttaa ttagtatctt atcacctttt gtatatccct tttgaactaa tgctgagaat
    14821 ccaccaataa aatctatacc taaatcctct gcagccttat ccaatgtctg tgcaaacttt
    14881 acatagtcag tttcatctgt tgcacctgca ataatggaaa ttggagttac tgataccctt
    14941 ttatttacaa ttgggatacc aaattcacgc tctatatctt ttccaacctt caccaaatct
    15001 ttagcagaac tcataatctt attgtatatt ttttctctag ctttatctcc atcagggtct
    15061 atacaatcta ataaagaaat ccccatagtt atagttctta tatccaactg ttcttcatct
    15121 atcatcttta ttgtttcaag tatatttcct acattcacta ttatcacctc cccttactgt
    15181 ctactacaga ttatgcattg aattaaatat ttcttctcgc tgaattttta catccactga
    15241 taatttctta cctctttcaa ccaagtcctc aaccgttttc tcaaaagaat ttaatgattt
    15301 ctctaaatca actatcataa tcatagtaaa ataatcctgt aatatttttt gtgatatatc
    15361 tactatgttg atattttgct tgtataattc atcactaatt cctgccacta tacctggttt
    15421 atcctttcca attactgtta aaattgcttt cataataata tcctccctta tttttttata
    15481 aatcttatat ttttacaaat tgaactatgt gttaacatat atttcattta tttgttagta
    15541 aaccactttt tatgtatata tttaatatat tttaatacct atacattcat ttgtcaattt
    15601 tttttcaata ttctattatt tttatactat ttagaaaatt catatattat atatgttttc
    15661 tcaacaacaa atacaatttt cataataatg aaaggtatta
//
LOCUS       DAFKMX010000097        15670 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFKMX010000097 DAFKMX010000000
VERSION     DAFKMX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.097"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(59..1246)
                     /locus_tag="LZB08_002313"
     CDS             complement(59..1246)
                     /locus_tag="LZB08_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7315810.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(1251..2321)
                     /locus_tag="LZB08_002314"
     CDS             complement(1251..2321)
                     /locus_tag="LZB08_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBM7315811.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            complement(2331..3653)
                     /locus_tag="LZB08_002315"
     CDS             complement(2331..3653)
                     /locus_tag="LZB08_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315812.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            complement(3685..4020)
                     /locus_tag="LZB08_002316"
     CDS             complement(3685..4020)
                     /locus_tag="LZB08_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315813.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            complement(4117..4422)
                     /locus_tag="LZB08_002317"
     CDS             complement(4117..4422)
                     /locus_tag="LZB08_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7315814.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            complement(4437..5480)
                     /gene="ypdE"
                     /locus_tag="LZB08_002318"
     CDS             complement(4437..5480)
                     /gene="ypdE"
                     /locus_tag="LZB08_002318"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBM7315815.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            complement(5483..7384)
                     /locus_tag="LZB08_002319"
     CDS             complement(5483..7384)
                     /locus_tag="LZB08_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7315816.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            complement(7710..8561)
                     /locus_tag="LZB08_002320"
     CDS             complement(7710..8561)
                     /locus_tag="LZB08_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBM7315817.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(8610..9779)
                     /locus_tag="LZB08_002321"
     CDS             complement(8610..9779)
                     /locus_tag="LZB08_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBM7315818.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            10241..11158
                     /locus_tag="LZB08_002322"
     CDS             10241..11158
                     /locus_tag="LZB08_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBM7315819.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(11287..12216)
                     /gene="pfkB"
                     /locus_tag="LZB08_002323"
     CDS             complement(11287..12216)
                     /gene="pfkB"
                     /locus_tag="LZB08_002323"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBM7315820.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            12485..13210
                     /locus_tag="LZB08_002324"
     CDS             12485..13210
                     /locus_tag="LZB08_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7315821.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            13293..14447
                     /gene="nagA"
                     /locus_tag="LZB08_002325"
     CDS             13293..14447
                     /gene="nagA"
                     /locus_tag="LZB08_002325"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBM7315822.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQETPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKNPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(15146..15628)
                     /locus_tag="LZB08_002326"
     CDS             complement(15146..15628)
                     /locus_tag="LZB08_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBM7315823.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT         5236 a         2564 c         1824 g         6046 t
ORIGIN      
        1 aatatttgat aactaaataa tgtgttttta tataaaccat agatactttt tttatgattt
       61 aacaatacat acttatatac gtttttttga ttctatttag agcctcattt aatgtttctc
      121 taggacaccc cacattaata cgaataaatc catctccatc tttcacaaaa taacttccaa
      181 ctaccatagt taagtttgca tgtctaaaaa attctttaga ttcttcttca tttctaaata
      241 cttctcttac atctatccag gctaaaaaag aagagtctga cttcatcact ttagcttttg
      301 gtatattagt ttcaaagaaa tcataaacta tttcaaaatt ctcctctaca taactagtca
      361 tagcatctag ccattcttcg ctttcattat atgctgcaac tattgctggt attgcaaata
      421 catttggaga agttattgaa ttacttttta gcctttctaa taaagtctcc ctaattttta
      481 catctcttat taatacatat gatgttttta atccacctaa gttgaagcct ttattaggag
      541 atacacagat tatactattt ttataaattt ctggatgagc attccacaaa gatacaaatc
      601 tataatcttt aaatattata tctctatgaa tttcatcact taccaaaatt acattatact
      661 ttaaacataa ctctgacact tgatatagtt cttcttttgt ccatatacga cctgatggat
      721 tttgtggact acagaatata agcaatctta tattctcgta tttcatttga ttttccatat
      781 cctctaaatc taaataatat ctattgttat ctaattttaa tgagctacaa cacaactttc
      841 tattattatt tactacagcc tctgcaaaag ggtcataagc aggtgtattt atcaatacac
      901 cctctccttc tttagtatat gcttggacta tataatgaag ggttgaaaca gtaccaaaag
      961 ttaattttat ccaatccttt tctatatata catcaaatct tcttttattc caatttataa
     1021 tagcatcata gaagtcatct cctataaaag tataactata atctcctact gatgctcttt
     1081 ttaagataga ttcttttata gcaggagcac actcaaaatc gatatcagct aaatccatag
     1141 gaattgcatc ttcatttaag ccaaattttt ctttaatcac atggttgctc cattttctac
     1201 aatttccttc tttacgattt aatactttat taaaattata tttcataatt ttactctcct
     1261 aatattataa actctttgga tgtcttattg agtggaacac cttttccatc tattataaca
     1321 atatcatctt ctattctaac tccccctaca tttggaatat aaatacctgg ctcacaagac
     1381 attaccattc cttccactaa tatatcttca ctatttggat ttaaagtggg gacttcacca
     1441 tctccaagac ctataccatg acccagtcca tgattaaaat attcgccaaa tccatgttta
     1501 tctataattt ttctagccac tttgtcaaca tcacttgccc tagttccttc ctttattgac
     1561 tctattgcag ataattgtgc ttccaatact acatcatata tctctttaat aattttagga
     1621 ggtttaccta tagatattgt acgagtcatg tctgattgat acccttgata aacaacacca
     1681 aaatctattg ttatagcttc attcactttc aattttttgt ttgtaggtct tccatgtggc
     1741 attgcacctc tttctcctga taccactatt gtttcaaaag acattccact tgcacctttt
     1801 tttaatgcat gatactgaat tagagctgat acttctagct cagtcatatc ttttttaatt
     1861 tcagaaataa cctcatcaaa tacttcatct gtaatctcac aagccttttt aataagttca
     1921 atttcttctt tagattttat agcccttagc ttccataatt catttgttat taaattgatt
     1981 tcaaaacctt cattttttag tgaaagatac tcttgtatac tcatagcttg gttttctatt
     2041 gctaattttt ttatatttaa ttcttttaat aactcaatta tagttggtat ataagaacct
     2101 tgagatacaa tcctttttat gaaaccagtc gtttttttat ctgcttcatc aatatatctg
     2161 ccatctagaa tttgataatt tttatcctta gtaacaagca catatacacc actacctgat
     2221 aaagctccta tatatctttt agtatttttt gctttaatca aaagagcgtc aacacctctt
     2281 ttatagatta aggatactaa tctctccatt gtattattca taacaaaata ttagaaatcc
     2341 aatcctaaat catccaataa gtcttcttct tctttagaga atatattctt agtttcttct
     2401 ttttgttttt tattattttg ttgacgttcc attattttaa agaatggcca atataataaa
     2461 actgctaata ctaattgcaa tgcatttgct aatatagaat gccaatctcc attagttaaa
     2521 aatccttcta agaatacccc tacatatgga gggtcaaata ctggctttgc taaccatccc
     2581 cagtgcataa aaaacattgg tagtgtacct attatagctc caccaaatac atgtggtata
     2641 aacattattg gatttaatat tatcggtgct ccaaataata ttggttcatt tatcccaaat
     2701 aatgcaggga ataatgatac acgtccaatc ttcttatagg aagcattttt tgataacata
     2761 catgcaatta cacaacctat actaattcca catcctgtaa atccaaagaa tgcacttgaa
     2821 gctcctgctg taaagaaatg aggtattgtt tctccagctt gcatagctgc tatgttttca
     2881 gctataaatg taatcattat aggtctagtt ataggactaa atactgatga atgtattcca
     2941 aaaaagaata tagtacatat agcaaagtta agtgcaagaa ctgaccatgg gttatccata
     3001 cttcctacta atggagataa aaattgagta agtatctgtg gaggtaattc tccaaatact
     3061 tttaatgaaa taaaacgagc aactataaaa gttccacaaa ctattatagt agtaggtatt
     3121 aattcaaaag acctagatac aaaatcaggt acattttctg gcatttttat tgtgatattc
     3181 ttcttattac aaaaattata taactccaca gaaccaagcc ctgctatcat acctgtaaat
     3241 agccctttag ctcctaaatt tcctatttct aaagctccat ctggtgtaaa tgaggctact
     3301 aggaataaaa aagaaaaagt tgatagtgca acacaacctg gtatatataa tttatagtag
     3361 ttccccagat taaaagcaat acacatacaa gcatataatc ctattaaacc tatggaaaga
     3421 cttactggta aatccaatat tgctgaattg ttaagtatga atttgcttat tggattattt
     3481 tctccaagca tatttggaag tgctggaagt attgcaaata tacttcctaa tattgtgaat
     3541 ggtgtcatag caaccatacc agcctttata gaaccaaggt gacgttgatt gtcaacctta
     3601 tgagctaaag gcataaatac tctattcata aatgcttcaa attttgaaaa catatctaat
     3661 ctcccttttc cctcattatt tttattatcc ttgagctaat tgatgtttct tataagttat
     3721 taatgccaac ttcataactg tagctccatc catttttcca tatacatctt tatctattac
     3781 tgcaaaaggc ttattttttg gctctaatat tccttttaag aagtctattt tgtgagctat
     3841 ttgaggacct ataagtacta tatcccaatc atcaacttca ctttctattg aatctgctgg
     3901 tatagctgta aactcaaaat ctttttcatt gagtttagca ctctctttta ccaccttttt
     3961 catattttgc attaacatat tagtagaaaa tccacctgca caacataata atattttcat
     4021 aataattcct cctaaagttt gtatttttat tatctcttaa aagcatctaa acttttaaaa
     4081 gaatttttac ataaataatc tatttctaca ttacaattac ttatcgagac aatatatcaa
     4141 agcctctatc aagtcttttg ctagctgaga agtcatatag tggtcttgag catgtaccat
     4201 taaaagactc attgctggac tttcaacatc ttcatttaaa tcttgagtta ttaattctgt
     4261 ttgtatcctg tgagcttcaa tatctatttt acgagcctcc tctattaaca attttgctcc
     4321 tttaaaatct tttgacttca ctttttttaa agcctcaaaa gctttcgatt tactctctcc
     4381 tgcagttgat ataagttcca ttatttgtac ttctgttgac attttcttat tctcctttag
     4441 ataaaatttc ttatatgttt atattgttct tcatcaaaat ttaaaattaa ctgtgtatac
     4501 aatcttataa tgttctccaa atctttatta cttactattg aaaatgcaca atgaccatat
     4561 ctaactggta cacaagttac tacacaaggt ttcccttcat ttacgagatg tgcttctcct
     4621 ccatcagtcc cacctgattc aaacatatca tgttgaagtg gtatattatt tttatcagca
     4681 atttccatta cataatctat catttttcta ctagggacta gagtcctatc tctatgcatt
     4741 agcattacac ctttatcaat ttgtttctga tttgtatagt ctctcacaaa ctcattttta
     4801 gcacatgcta catctattac aaatactata tctggattta ttaactgtgt tgatgtcttt
     4861 gctcccctta ttccaacctc ttcactactt gtagccgcaa agtgaatatt acaatttaga
     4921 ttttctttcc taagttcttt taaaacttct cccatcacaa aacatcctat cctatcatca
     4981 aaagcctttc ccactaatcg gtctggtaaa gtaaattcct caaagcttgt tgtatatgtt
     5041 accatatccc caacattaat ccctaggtta tatacatctt cttctgtata tgctccaata
     5101 tctgcatata tattttcagc cttaccatta ttatatgtag catttatgac agcggggatt
     5161 tttttccctt cttttgttgt aattctaact ttttgtacaa actgtgcaag aggttttact
     5221 cctcctattg ttattaagtg aatcatacca agtttgtcta tacttctcac cataaatcca
     5281 acttcatcca tatgagcaca aatcattacg tttggtgcac tctcatcttt tattttagaa
     5341 aagatgatac tccctagccc atcacagata attttatcac tataagcttt tagttcttcc
     5401 aataaaacat gtctaacctc ttcttcatta gatgctatcc catctgcttc tgacaacctc
     5461 ttaaaaaact ctatattcat gtctaacctc ccagatagat ttttaactta tacataaact
     5521 cttcataaga ttgtgactta tttagcatgt ctaccatatc cttatttgcc ataatcttac
     5581 ttaaatattt agatatctgc ttatgaagtt ctaaatttcc ctttattaat gaaatcatga
     5641 aaataatttt gggcacccta ttttcataaa taatatcagt ttgtatcaaa gctactgata
     5701 ttatattttt gtttcctgtc atctcgattg gatgaggtat agccacccca tttgtataaa
     5761 tagtactcaa atagctttct ctttcccata catctttggg ataggttgga gaacaagctc
     5821 cctcgtcaac tatacatttt gacatactat caaggatatc cttatactta gctttatctt
     5881 ttatacacct aaagtgattc ttatcaaata gacctaatat atattgtttt gggcttgata
     5941 tatttcctat atggtctgca aatctaacac tttctttaat atctaaataa tccaattcgt
     6001 ctaaaatttc atttataagt ataactggag catttatttc aaataaaaga tttgttattg
     6061 aaaaaatcaa atctggtgca aattctatta cagctttttc ttctagtaat gaaaaatttc
     6121 ttatctcagc atttggaaaa atttctttaa gtcttaaatt tatcaaaaat gcactacctc
     6181 caccagaaga gcatattatt gctattttat attttttatt aaaattagct tcttctctct
     6241 tttcaaaagg aacagcaatg tgagtagcta taaaccctat ttcatcttga cttattttta
     6301 tatgatactc attttctatg atttttgaaa attgaataga taaattaaaa acagttggat
     6361 actgttgact tattttaact gaaaaaggat ttattatctt gtggttcttc tttatacgtt
     6421 ctattaaaca tgcaatatgt aagtaaaata agttaaaaaa ctctttatct tctaaaaaat
     6481 tagagttata ttttttatct atctctctaa aaaattcgaa cataatatct tgaagtttat
     6541 tcttatcaat attctttata gtggctttct tatctttagt ctttagtttt ataagctcct
     6601 ttaaataata cttttcatca taattaaggt tatatttttt atctctaaaa attttatcta
     6661 ataatttatc tataattaaa tcttctgtat ataattttaa atcgctagca tttcccacta
     6721 ctctcatact aactcttata tacaatataa taatttgtgc taatatttcc tcaagttctg
     6781 ttaaatttgc ttctaaatca tgttcattaa gtacattttt tattgtactt ctaatattag
     6841 ctaattttaa attatctata aacttattcc tgtactctgt tatatttcta ttccctctaa
     6901 aataactatc caccaatata ctttgaattt cttttataga accttctatt ttaattccat
     6961 aatgagcctt tcgctctagt tttaattctg tatcctttat cttttcatct atctttttta
     7021 aatcattttt tatttgggat gaacttacta gaaatttttt acttaactgt tctatagttt
     7081 tataaccttc atttactaaa agctctacta ttatagactc caaccgttgt tctggatttt
     7141 caactaaatc tgagggtaag tttcttagat aatgctcgaa tttatcttgg tctttaatat
     7201 ttagaatata tcctaattta gacttatatt ctaatttaaa accattacat tcttttatag
     7261 tttctatctt ttttatttca tttctgatag ttcgagtaga tacccccaac ttatctgcaa
     7321 aataatctat gtttagataa ccactactat tttttaatgt ttcaagaata atgcgttctc
     7381 tcatatcccc acctccctca gtgttattat tgtgtttttt aaagtggtat aaaaaatatt
     7441 ttttataaaa acaacctttt ctttttattt aagaatttta tatcctagta tattttaatt
     7501 ccacaattta atataaatat tttataataa taaaatatat agttcaattt ttagtatatt
     7561 ttaattatat atgtaatact ttaccaataa aagctgatat ttttcatcaa ttaaatgaaa
     7621 aaaatacacc atttacatat aattaatata aatttttcat ataacaaata tcctaaactt
     7681 atctaaaata aatttaggat attttatttt tatactctat ttacagaccc acacacttta
     7741 atcttttctt tcacaaaatc ctttactgat tgaagccctg ctaatccata ctttttaggg
     7801 tctataacct cagaatcttc atctaaaact tttttaaccc ctttactata tgcaattctt
     7861 aattcagttg catagttaac ttttgcaata cctcttttta tagcttcttt tataatatca
     7921 tcatgtattc ctgaacctcc atgcaatact aaaggtattg aaactacatt tttaatttct
     7981 gataacctat ctaaatctat tttaggttca cccttataca atccatgtgc agtacctata
     8041 gcaatagcca atgaagatat tcctgttcta tcgacaaact cttttgcctc ttttggattt
     8101 gtataatcat tactattccc gccatctaaa tcatcttctt ttccaccaac tttcccaagt
     8161 tctgcttcca ctggtataag agaaggacta catgctttta ctactgattt agatatagat
     8221 atattatttt caaagctttc atgagaacca tctatcatta ttgatgtata tccacttctt
     8281 aaagctttca ttgcaaggtc aaaacttgaa ccatggtcca gatgcattgc tacaggtata
     8341 tttactcttt ctgcagcaac ctttgcattt gctagataat aatcaagacc tgcatatttt
     8401 acagtagatg gagtagtttg taatattact ggagaattta actcctcagc agcttctata
     8461 acagcaatta ccatttccat attttctaca ttaaatgctc ctactgcata cccttcatct
     8521 tgtgcctttt taagcattga tgttgttgtt actaatgcca cattgatacc ctcccaaatt
     8581 atttagttat aatattttaa tcttatcttt taatattcat atattgttac accttttact
     8641 actcgattta ctgttcctgt agctgatggc gtatctggct tatttccaac ctttaccgat
     8701 acaaacaatg ctatagtttg tgcaaacaat atgtatggca ttgcaagata cacatctggt
     8761 aatttattat acttttcttt aaactcaaac tttgttcctg agaagttatt ttcattttct
     8821 actgacactg cacaagtgaa tttagcaatt ttatcccttt ttatctcctc taaaacatct
     8881 aaatcatatt ttcttgtata taaacagtta gatacaaatt caaatacaag tgtattttca
     8941 tcaataaatg attttggtcc gtgtctaaac cccataggag aatcatacac tgtagatatt
     9001 tttcctgctg taagttctaa aagttttagt tgtgcttctt gtgtcaaacc acctaatcct
     9061 cctgaaccta aatatacaac tctatcaaaa tctttattta ttaactcatg tatttcatct
     9121 tttctatcta taacatttct tcccatttca attattgcat ttatatatga cttttctaca
     9181 tcatcttcta aactatcgaa tattaacata gcagataaca tcatacacga aaaacttcct
     9241 gtcatggcaa agccttcatc atttgatttt gatggcatta ataacaagta attattttcg
     9301 tcgtttttag tcatcttagc cagttctcct tctggattac aagttatagc taaatgataa
     9361 aaattatcta caatcttatt ccctaaattt aaagcagcca aactttcagg actatttcca
     9421 cttcttgcaa atgaaattaa taatgttggt atatcttttt ttaagtaatc atatgggttt
     9481 gaaactatat ccgtagtagg tattgcttca aatatgtatt ttcttatgtc atttttattt
     9541 ttaaggtaag gaagtattga atttcctatg taagctgaag ttccagcacc tgtaaatata
     9601 actctaaact gcccatgatt attacttatt gtatctatga atctacttaa tttttctttg
     9661 ttagatttat atatttcata agtctctaac cataattcag gctgttgttt tatttcatta
     9721 gttgttatta ttgcacctaa atcttttagt ttttgttctt ctaatttaaa cattttcatt
     9781 ctcccttcat acttgtaaca tttacatttg gtaatgaggt tataaccact ttaaactata
     9841 aaaaaatata ctcttttata attttttgct tagatttttt agtactactt aaactcacta
     9901 taaaatatac gctatagatt ttaactaatt ttatagttta tctttaaaaa tttaataaaa
     9961 tcatacttta gtatttttat tttacgataa aaacttgttc agaccaaagt ggtaattacc
    10021 actaatccaa atataaatta atttgaattt tttgtcaata cataaaatat ttatatcaat
    10081 caattttata gatatcctac aaaattgctt tatcaatatt tcttatttcg ctaatttact
    10141 tatataagct ttaaatttat tttttgatac aaagtttaca aatttatgtg tctgaattat
    10201 aatcaattct gtaaatataa aaaccttagg agaaagtatt atgaaaaata taaaaatggg
    10261 aatgataggt cttggctcca tagctcaaaa ggcgtattta cctatattaa ctaagtctga
    10321 gagatttgag tttgtaggag cttttactcc aaataaagtt aaaagggaaa aaatctgtag
    10381 tgattataga atcatgccat tcgattctat tgaatcccta gctaaaaaat gtgattgtat
    10441 ttttctacat agctctacag aaacacacta cgaaattata aaaatattat taaatttagg
    10501 tgttcatgta tatgtagaca aacctcttgc ttcaacagta agtcaaggag aagaacttat
    10561 cgaattaagt acgaaaaaaa acttaaattt aatggttgga ttcaaccgta gattctgtcc
    10621 tatgtacaaa gaaattaaaa ataatgctac agaaattgta tctatcaata tatgtaaaca
    10681 tggattaaat tccttaagaa atgttaaatt cgacagtact cttatagatg actatataca
    10741 tgttattgac actgctttat ggttagcaaa tgaagatgtt gaaattagtg gagaggattt
    10801 atttttaaca gacaataaga atcttatatt tgtaagtcat aaacttaaag gtaaaaattt
    10861 ctctataaac acctctatgc atagagattc tggaacaaag ttagaacaag ttgaaattct
    10921 ttcaaagggt aagattcaaa gagttaagaa tcttaatgta ctggaaatag aagaaggtgg
    10981 aaacctaact ttaaagcaaa gtggtgcatg ggtaaacata ttaaaacaaa agggttttga
    11041 agatatatca aatcatttta ttgattgtat agaaaataat ataaaaccag ctattaatgg
    11101 agaagagtgt atcaaggctc aaagattact agagaaaatt ataaattcag taaaatagat
    11161 atttattata aaaataggtt aaaaatgaat ttttctaacc tatttttata taagactata
    11221 tcttttatat taaaattaaa atctattaca acaacattaa atttattata ttcccattaa
    11281 atgtatttaa cttaactttt ttacttcaac taaatcttga tatttatcaa tttctaccaa
    11341 atctacaaat ccagtttcca tatttaaagc attacttata ccacaagcat ttgcaaattt
    11401 aagtaaattt tctattttat aaccccttaa aatacctaca ctaaatccag ccactgtgct
    11461 atctccacta cctacagtat ttatacactt tacaactggt acttttacat catatacact
    11521 atcttcgttt aaatacacca taccatcttt tcctaaagat atacatacat tctctgcacc
    11581 catttcaata agtttttttc ctgacaagat aatttcctct cttgattcta tatttattcc
    11641 taataaatgt cttatttcat ctatatttgg ttttattaaa tatggttttg attttaaagc
    11701 tatttttaat gcttctcctg atgtatctaa tataaacttt atattatttg ctctacatat
    11761 atttcctatc ttttcataat aatctatcgg catattctca caataactac cagaagctac
    11821 cagtatttta gtatttttta gtacttcctc tatatcattt tcaaatctct ctaaatcgct
    11881 tttttctact attggtcctt tttctaaaaa ttctgtacta actttattgt cctcaattat
    11941 gtttaagcaa tttctagttt cctgctctat ttttgtaaat ttattttgta tatctagttt
    12001 tctaagttca ttttttataa attctccatt aaatccgcct agaaatccca tagctaaagg
    12061 attttctttt aaaattttac ataccttagc tacgtttaat cctttacctc cagcagttgc
    12121 attcttactt actacccttt gcacctcacc tatgtttata ttgttcactc tatacattct
    12181 atctatagat gggttgaatg ttattactgt aatcatacta gattctcctc attattaatt
    12241 tttacttttc taaacaataa attaatctgt aaaataaaaa atacctcatc tataatatat
    12301 ccaaagtggt tattaccaat ttgaatataa attattttac ttgttttgtc aatgtaccat
    12361 aattttataa taaattaata gccttactta ttgaattttg attaaacttt attcattgac
    12421 ataaataatt ctatattcta taatcaaatt gtggtaataa ccacttagaa aggactatat
    12481 ttttatgata aaaagaaatg ataaaagacc aatatatgac cagcttgttg agatattaag
    12541 aactaagatt gaaaatgaga tggaaccaaa cgatagaatg ctttccgaaa gaaaaatttg
    12601 tgatgaatat ggagtaagta gaaccacagt tagactagct atggctgagt tagagcatat
    12661 gggatatata tataaaagac atggaaaagg aacttttgtt gctgcactaa gtcaaaattc
    12721 tcaaaactta atggaaagtt atagctttac agattatatg agaacacaag gaaaaaagcc
    12781 aggtacaaag gtattatcat ttgaggtagt agaaagtaca aagtatttct ctgaaaaatt
    12841 aggtataact cctggtgaaa aaatgataaa aataatgcgt ttaagacttg ctgatgacct
    12901 tcctatgatg cttgaaagaa catatcttcc tatgaaagaa tttataggat tgacaaaaga
    12961 aaaaatcgaa caaaaacctt tatatgaaat atttagagaa gattatggcg agattattaa
    13021 agttgctgat gaagaatttt cagctggaat aatctctgat aaggaagcta agttattgga
    13081 agtaccagga gattcacctt gtctgaaatt acttcgtacc acatataatg atgacaatat
    13141 agttattgaa tttactttga gtgtcgctag aagtgataaa tttgtatata gagtaagaca
    13201 tgtaagatag taagttaaat aactttcaag aaaacagaat gatattataa gcgtcaatgc
    13261 cacaatgata gtataattat aaggaggtta ttttgaaaag ttttatttat gcagataaat
    13321 tttttatgaa acatggaatc aaagaaaaag gttaccttag tataattaat ggcaaattcg
    13381 gtgaatttca acaagaaaca cctaatgtta ctgataatgt tatagatttt tcaggtaaat
    13441 atattgctcc tggcttagtt gatactcaca ttcacggtct tttaggtgca gatgtcatgg
    13501 acaatacatt tgaagcaata aacacaattt caaaaggctt attaaaatat ggagtaacat
    13561 ctttcctacc tacaacttta actgattctg tagatacact aagtgattct gtagaaaata
    13621 taagcaatag cttcaatcga gttgagggtg caaaaataca aggtatttat ttagaaggtc
    13681 cattttttac agaaaaatat aagggcgctc aaaatgaaaa atattttaaa aaccctgaca
    13741 tcaatatatt aaaattatgg caaaaaaaat cgaaaaattt aataagaaaa atagcaattg
    13801 ctccagaacg taaaggttca atagaattaa caagatatgc aactaaaaat aatatatcag
    13861 tttctcttgg tcatagttca gctacatttg agcaagttaa acaagtcgtt aattcaggtg
    13921 ctaaagtgtt tgtacataca tataatggga tgagtccttt aaatcataga gaacctggaa
    13981 tggttggggc tgctatggta ctaaaagata cttatgctga attaatttgt gatggtcatc
    14041 atgtaagccc aatagctgtt aaaataatga tggatgctaa gtctagtaat aacatagctt
    14101 taattacaga ttgtatgaga gctggtgcta tggaggatgg aaaatatact cttggtaagt
    14161 ttaatgtaaa tgtaaaaaat agtgttgcaa gattaaatag tgggagttta gctggaagtg
    14221 tattaactat ggacaaggct gtgagaaata tagttgattg gaacatatca actttagaag
    14281 atgcaataaa aatggctaca tatgtccctg ctttaagttg taatattgat aatgtctgtg
    14341 gctctatata tcaaggtatg gatgcagact ttatagtaac agataatgac tttaatatac
    14401 acgaaactta tataaatggt atctgtaagt acaaagttaa taaataggtt caatataagg
    14461 ttatggaggc tgtctaaaaa gagtctggaa atatttaaag ttatttctcc aaaatctgat
    14521 ttaacacttt tatttccata tcgttaataa actattgcga tagaggttat tttaaacaaa
    14581 tatatattat aagtattttt gtgataatat tttcgataat atgtacattt aaagtaactt
    14641 ttctattatt atttgtaagt ttttatcttg aacaaacata taaattttta ataaagcatg
    14701 agataaaccc tatatttcaa ttactaatga tgagattttg attgataaca gatatatttt
    14761 caaatgtaaa aatactaaaa atcatatcaa ttttaaagat attgtaggtg taaataaaca
    14821 agataatact atagaaattt ttacaaatga atgcacttat tgtatatatc taaaagtttt
    14881 aagcattgag agcgaagaat atctaataga tagactaaaa tcattttgtt gattttatat
    14941 attttatgtt aaatataaag cagagtagtt aaaactaccc tgcttttgta attaaaattt
    15001 aaaaataata actcataata cattgaattt gagcttaaca gtaactattg taataatagc
    15061 atcaatcttt tgtgttgaat ataatttatt tacactatag ttaagcatga tttctaatgc
    15121 tattttctct aatttacaat tatgattaaa attcattaat atatatatat ttataagttg
    15181 tattgtttat attgtttatt acttttttaa atccactagc tgtaataata aatgtctttt
    15241 ctggtatccc tggtgaaaag attattttat catggttaaa tatatcttga tctattacaa
    15301 gagagatatt tttctcatat ccaaaaggag atacacatcc aggcaaacat tgtgtttttt
    15361 gtgttaattc ttcttttgaa catatagata cttttccacc taaaacattt tttatagttt
    15421 ttggatctaa tttttttcct tctgtggtta caaatatata gtaatttcca gacctatctt
    15481 ttataaataa acttttactt tctactccag tatattgtaa tttttctctt atttctttag
    15541 ctttttcaaa tgataaaata ggttcatggt catattcttc aaacgttata tgattttctt
    15601 ttaaaatatt tttgatattg ttgtacatat attttccttt ctatatttaa attaatttat
    15661 atataatatt
//
LOCUS       DAFKMX010000098        15635 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFKMX010000098 DAFKMX010000000
VERSION     DAFKMX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.098"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..355
                     /locus_tag="LZB08_002327"
     CDS             <1..355
                     /locus_tag="LZB08_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBM7315824.1"
                     /translation="VNKKALSEKLKCKKTVNFLKFLGYKQNTNVNAYLEHLVDKLKSD
                     VFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWKVYGDTKQSEDTYSKFLLHRKKMR
                     KLLDYISVDKIVSCF"
     gene            complement(453..1412)
                     /locus_tag="LZB08_002328"
     CDS             complement(453..1412)
                     /locus_tag="LZB08_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7315825.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1446..2177)
                     /locus_tag="LZB08_002329"
     CDS             complement(1446..2177)
                     /locus_tag="LZB08_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315826.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2170..2940)
                     /locus_tag="LZB08_002330"
     CDS             complement(2170..2940)
                     /locus_tag="LZB08_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315827.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3025..3735)
                     /locus_tag="LZB08_002331"
     CDS             complement(3025..3735)
                     /locus_tag="LZB08_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBM7315828.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(3915..4067)
                     /locus_tag="LZB08_002332"
     CDS             complement(3915..4067)
                     /locus_tag="LZB08_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBM7315829.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4093..6249)
                     /gene="feoB"
                     /locus_tag="LZB08_002333"
     CDS             complement(4093..6249)
                     /gene="feoB"
                     /locus_tag="LZB08_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBM7315830.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6349..6570)
                     /locus_tag="LZB08_002334"
     CDS             complement(6349..6570)
                     /locus_tag="LZB08_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBM7315831.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6583..6795)
                     /locus_tag="LZB08_002335"
     CDS             complement(6583..6795)
                     /locus_tag="LZB08_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBM7315832.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7116..10013)
                     /locus_tag="LZB08_002336"
     CDS             complement(7116..10013)
                     /locus_tag="LZB08_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7315833.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10340..10948)
                     /locus_tag="LZB08_002337"
     CDS             complement(10340..10948)
                     /locus_tag="LZB08_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBM7315834.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11208..11753)
                     /locus_tag="LZB08_002338"
     CDS             complement(11208..11753)
                     /locus_tag="LZB08_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBM7315835.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(11871..13694)
                     /locus_tag="LZB08_002339"
     CDS             complement(11871..13694)
                     /locus_tag="LZB08_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315836.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMCEGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(13707..15482)
                     /locus_tag="LZB08_002340"
     CDS             complement(13707..15482)
                     /locus_tag="LZB08_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315837.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5169 a         2823 c         1527 g         6116 t
ORIGIN      
        1 tgtaaataaa aaagcattat cagaaaaatt aaaatgtaaa aaaacagtaa actttttaaa
       61 gtttttagga tacaaacaaa atacgaatgt taatgcttat ttagagcatt tagtagataa
      121 acttaagagt gatgtttttc ctgatgaaat aggtattttt ttaggatatc ctcttaaaga
      181 tgtgattgga tttatgggat atagtaatta tgaagtttct atgattaaat attggaaagt
      241 ttatggtgac acaaaacaat ccgaggacac atattcgaag tttttacttc accgtaagaa
      301 aatgagaaaa ttactagact acataagtgt agataaaata gtaagctgtt tttaattgtg
      361 tttttgtata ataataaaaa tacaccctct tttaccatac agtttccata ataaataagg
      421 gtgtattttc cactttaata ttttaaagta ttctaatttt gattgtacaa catatcttta
      481 atatttattt tcttttgttg cattccatta gacatcaaaa attcctgtgt atcttctaat
      541 tcttttatat ccttgtccgt tattttagaa ctaaagtcat accaagaata catttctttt
      601 gtttcatcta aagataaacc aacttcttta gatacaacat ccatcacttc atccttgttt
      661 tcattcatat attttaaagt ttcttcatga accttcataa atctttttac taattcagga
      721 tatttttcag caaagtcatc acttacagca gtaactatta ttccacttac taatccttct
      781 ccatttacaa tcattttggc acctgaattt atcgttttca atgccactgg acctgcaagc
      841 attgctacat cagcactttt actttctaat gcagaagcag cactaggaat atccatattt
      901 acatattcaa catcatctat agtatagcct tctttagcaa gtgcagcaat caacagttga
      961 tgaagtatag tcccttttgg acctacaatt tttttacctt ttaagtcttt tatagattta
     1021 atattatcat tattagttat aagcatatat ccttttggtg aacggctata aatattcgta
     1081 attttaagac caacaccatt tgatgcagct attattgcag acgttcctcc taatgcatgt
     1141 aaaaagtcta gctcacctgc tgctaatgct tgagtttgct ctggtcctgt tgtcaattca
     1201 tggaagttga cttttatatt gtccttttta aattcctttc caaataaatc atcctttttt
     1261 tctaatatac taggaacatt aagtggtgat tttacataag ttaaatttat ttccttaggt
     1321 aaactagcct ctttttttgc aggttcatct ttcttatcat ttgttgttga acatcctaca
     1381 gtcccaacca acaccattat taaagatgtc attactgcta aaatcttttt gtttctttta
     1441 atcaattaaa actcccccaa ttcccttaat atttcttttt tcaagtttat ataatagttt
     1501 ttagttaaat ctctattata ttcatcttcg acactaaatt gtttaatcct tttattttta
     1561 ctaaaaacaa ttattttctt tgctatttcc atagcttctt caatattgtg agttacaaat
     1621 actacacctt ttttagtatt tttatggata tttacaactt ctttttgcat ttttcttctt
     1681 gtgaagtaat ctagtgctgc aaatggttcg tccatcagca aaatatcggg attgaaggaa
     1741 agtgctcttg ctatacttac tctgtgagcc attcctcctg ataattcatt tggatatgag
     1801 tttttaaact tttcaagctt catcattttt aaatacttgt ctaaatcaac tttatttcta
     1861 ttatcttttt cagtatgtaa aagtatattt tcacttacat tcagccatgg cataagtctg
     1921 ctttcttgaa ataccattcc tacttttgga gtacactttt tatcattatt aaaaaaatat
     1981 atgtttccac tagtagcatt ttcaagccct gctaaaattc taagtagcgt tgttttccca
     2041 catccacttt cacctaatat aacagttatc tcttcactag atatatttaa agatatatta
     2101 tcaagaacta aatgctcttt attatcaact aaatatttct tatatatatt ctctagcaaa
     2161 aatccacttc taaccattta aatcaacctg cttccctttg ctaaattttg ttattatctt
     2221 tgaaaataga taatctgaaa gtatacctaa aaatccaata acaaaaattc ctaccaatac
     2281 aacatcagtt ctagacatat cttttccatc agaaatcaaa tatcctagtc ctgaagaagc
     2341 tgcaataagc tctgctccaa ttatagccct aaaactatac ccaagcccta aactaagtcc
     2401 aacaacaata tccagcatag aattagggat tattatttta taaaaaattc tctttttaga
     2461 taaattaaaa gatttaccta cttctattaa tttattgtca caatttttta tacctttcaa
     2521 tgtatttaaa aatattggaa aaaaggatgc caaaataata attataattt tagattcttc
     2581 acctatacca aaccataaaa taagcattgg caccaaagct agtggaggtg tacttcgcat
     2641 aaattcaaat agtggtttga aatattcata cattttttta tttactccaa atacaactcc
     2701 aagtggtata gccatcaatg aacttattaa aaaacctatt aatatccttc ttacactggc
     2761 ataaacattt aagaaaatag aaccgtcata tatcattttt ataaaagtat ctaagactat
     2821 ttcaggagga ggcagaatat agctactcca tatacccaaa acacatacta tcttccataa
     2881 aattaaaatt attaaaaaaa tataatatcc tttaagcctt ttatgaattt tctcaaacat
     2941 ataaaactcc ttttaacata tctatccctg ctaccctttt tattttttaa tatcttaaat
     3001 aataattaag ctatatcata agatttaatc tatgccaagt cttaatccac ctgaactcat
     3061 tctatcagaa ggtgtagaat ctgtttttaa gccagacaat ctttcagatg gttgtataat
     3121 gactatttgt ccaggttcaa caaattccat atgataagat tctcctgaag tctgaccaat
     3181 aaaagtcttc catgatagtt ttgatacttt aggatatggt tctcttccag tccaagctac
     3241 taatgcatct gggtcaacac aacaaggtcc ttcaagaatt agaggattac catctgttat
     3301 tatagcaaca tcttgatttt gacctgtttt atttactaaa tgtgtagtaa aaagaccttt
     3361 ttgagatatt acccctgaac caatcaattt tacactgtaa tttaattgtt cagaaaatgc
     3421 caataagttt tcactctcaa cataaatgga atcacctggg tcaattgata ttacatctac
     3481 atggtaagca ttttgagcta ggtaaatttc accttcacct tcaaccacca ttattggcat
     3541 ttgttctcct gtcaatgacc tctgaacatg acctaataat gctcttccca atccaccgcc
     3601 attacttgga cctaacagca atttttcaaa attaaaattt cctttaaagg ctaccatagc
     3661 accttttttt gcaaaaaatt taccacttcc ccttgctata cacataagct cgtttgtaac
     3721 atcaaattta aacataatat tcacttatat tgcttaattt agcaataatc cctcctaatc
     3781 atatatttat atataattat acagtaaaat tacagcatag tctaacctac ttcaaatatg
     3841 aaatttgtta tttatacaaa aaataagaac ctgataataa agcaatttaa ctttttatca
     3901 ggctcttatc ctatttatct cttagcgcta tgacaactct tactacttga gcatccagaa
     3961 caaccacatc cacaactgct accattcttt tttgaatttt taaccatata cattacagct
     4021 agtgccatta atactactac aatagctgct ataataaatg tagccatatt catcactcct
     4081 tatttatatg aattaatttt tcatataact taatttaccc attttttctt ttttcatatt
     4141 tttatttgaa taagttaaaa ctacaaagac tactgctact gctataaata tagatattat
     4201 tgccccagca atacttcctc cagtacctaa aactaaactt ccaacttgat ttataagaag
     4261 agcaattata tacgctgtta aagtttggaa tcctaatgta atccatgtcc atttccatga
     4321 acccatttct ctttttatgg ctccaatagc tgcaaaacaa ggtgcacaaa gcatattaaa
     4381 tgccataaat gcaaacgcac tagctactgt aaacatgctt gcaactgaag caagaagtgt
     4441 tgggtcttgc tctgttgcat ctgatatacc aaataaaact ccaaatgttc caactacatt
     4501 ttccttagct actagacctg taactgttgc tactgatgat tgccaatttc caaatccaag
     4561 aggagcaaat attggtgcaa ctatatttcc taagctagct aatatactat ctccagcatc
     4621 taccatttgt aatgaccaat taaatgattg taagaaccag attactccac aagcaacaaa
     4681 tataattgtt cctgctttga ttataaatgc ttttcctcta tcccacatat gtattaaaac
     4741 accttttgca cttggaatat gatattgagg taattccata acaaatggag atggttctcc
     4801 tgcaaataaa ctagttttct ttaatataat tccacaaatg attatcattg caatacctaa
     4861 gaaatacatt gatggagcta cccatgaagc acctccaaat aatgcccctg caaataatgc
     4921 tattattgga atcttagctc cacatggaat gaatgtagtt aacataatag tcatttttct
     4981 atctctatca ttctctatag tacgagttga cataactcct ggaactccac aacctgaact
     5041 tataagcatt ggtataaatg attttcctga aagaccaaac ttacggaaaa ttctatccat
     5101 tataaaagct acacgtgaca tgtacccaca atcttctaat attgataata ataagaataa
     5161 aagcatgatt tgtggtacaa atcctagtac tgctccaact ccaccaatta gaccatcaac
     5221 tacaagtcct tgtaaccact ctgctacatt taaagaagct agaaaattac ttacattgcc
     5281 ttgaataatt tctccaaaca gtacatcatt tgtccaatca gttgctatag tacccaatga
     5341 acttactgct atatagtaaa ctccccacat tataagtgca aaaataggta atgctagtat
     5401 acgatttgtt acaatcttat ctatcttatc tgatacagtt tccttacctt ttcgattctt
     5461 ctttattata cttgaaatta ttgaactaat aaattcataa cgattggctg ttattatact
     5521 ttcactatca tcatccaatt catcttcaca atttctagtt atttcttcaa tactattcaa
     5581 tattgtttta gaaatatcaa gtttttgtat aacattttca tctctctcaa atagctttat
     5641 agctaaccag cgtgtttcta catctatata tggagtcttt tcttctatta ttttttcaat
     5701 tttttctatt gcatccttac tttcatcaac aaagggtaat ttgaaattta atttattgtt
     5761 actacttgca agttcaactg ccttttcagc agcctccata attccttgac ctttaactgc
     5821 tgttacttct accactgggc aacctataac ttcagatagt tctttaatat ttatcttatc
     5881 accattctta tttacaatat ccatcatatt aagagctata acggtcggta tacctagctc
     5941 taaaacttga gttgtaagat ataaatttct ctctatattt gatgcatcta caatatttat
     6001 tactgcatcc ggcttatcat ctagcataaa attacgtgta actacttctt ctaatgtata
     6061 tggagataaa gaataaatac ctggtaaatc tactatttcc acatctttat ttccttttaa
     6121 ttttccacct tttttttcaa ctgttactcc tggccagtta ccaacatatt gtgatgagcc
     6181 tgtaagccca ttgaacattg ttgtcttacc acagttagga tttcctatga gacctatttt
     6241 tattgacatt tccattccct ccattattat ttaaatacat cactgtttgt gatacagaaa
     6301 atcattctca ttatataatt acattaaccc tactctaatt aatctttatt attccacaat
     6361 aattttttct gcatctgact tacgaattga tagttcataa tctctgactg ttacctcaat
     6421 tgggtcacca agaggagcta cttttcttat aaatatatta acacctcttg taatccccat
     6481 atccattata cgtcttcgtg tagctccttc tccttctaac tttttgactt ttacagtctc
     6541 tccacactta atatccttca gtctgttcat gtcattacct ccctatatta ttattctact
     6601 tgccattttt ttatctaatg caactctagt atcttttacg tctattatta agttaccatc
     6661 tatcttagaa actattttaa catttgtacc cactacaaaa cctatactat ttaaaaaagt
     6721 cctcgtctta tcacttcccg aaattttctt tataactact tcttcacctg tatttgataa
     6781 tatcaatggc atcatataat atccctcctc ttaaagtaat ttaaatctac aagcaatcgt
     6841 tatcttatat ataaattaca ataccttaaa tcttaatcta ttaaaataat atcctatttt
     6901 ttccttcttt ttacaattcc taattttgat ttaagtctat cacaatagta tattttttgc
     6961 aagtgattat tgtattcttt tgatcttttt tctcatttga tttattttat taataaaatc
     7021 gtaaaaatta atacaaaaat aaagatgctc taaaatagta tactcaacta atattaaagc
     7081 acctttatta tatatgtcat tatttacaca ttgctttatt cttttttaaa agcgattttt
     7141 tcaaactctt ttacaggcat tggcttagaa aacaaatatc cttgtaccat atcacaccca
     7201 atttgcttta gaaattctac ttgcgaaata gtctctacac cttctgatat tactttcatt
     7261 ttcagttcct ttgccatttt aatgacatta gatataacaa ttttttcatt attactctca
     7321 tctttttctg taaaaaatgc tctatctagt tttaatatgt caacaggcaa ttcttttaaa
     7381 agattaagag aggaatatcc tgtaccaaaa tcgtccatag agattacaaa tcccttttct
     7441 ttcaaattgt tcattgtatc aagtaatcct tctatattat ctaatactgc tgtttctgtt
     7501 agctctaatt ctataagtct tgtcggaatt ttgtatttat ctacaattaa tttaaactcc
     7561 tctataaaat tactatttac aaaatgcatt cgagacaagt ttacagatat aggtactact
     7621 ttttgtcctt catccatcca ttctctaatc tttttacaca cttcctcaaa tacatagaaa
     7681 tcaatattta caataaaacc attcctctca aatacaggaa taaattctat tggtggtatt
     7741 agaccttttt gtggattatc ccaacgaaca agagcctctg cccctataat ttgataatct
     7801 gaaaggctat atttaggttg aaaataaaca ataaattctc cattattaag agcatctacc
     7861 attgaatttt caatttcttt ctcttttaat atttttttgt gcatttcctt atcgtaaaaa
     7921 gcaaatgaat ttttatgacc accttttata gtttttcttg cagtattagc tctatccata
     7981 atagtattta tgtcattatc acttttactt attttatata taccacaatc taaaatcaat
     8041 ttataatagt ttattttagg attactaagt atttgaacct tattgtaaat attgtttaac
     8101 cttgttttaa tatcatcatc tgtcttatac ttaataagaa gaacaaaatg gtcagcagaa
     8161 actcttgcaa aaatttcatc ttcctttaat tcacttgcaa tagtatttga tatatgtatc
     8221 aaagtatcat ttcccatatc atatccaaac atatcattta tatatttaaa tttgtcaaca
     8281 tctatataaa acaacgcata ttcttctgga ttattcttag caaacagttt attagaattt
     8341 attttaaatt tatctatact atctgctcct gtcaatgggt ctgtatatgc tatcttcctt
     8401 agataatcct ctttcttctt tttattataa attatatatt taatagcaat taagaataaa
     8461 atacatacaa aagaaataat ttgtattgaa tattctttaa aaatcacacc aaatggtact
     8521 ttataaggtt tgtcaatagt attagaatat atacaatcac ttaattcttc ttcagtaatt
     8581 ttgtctattg atttatttaa aatagacact aatatagggt cagttttatt tgatacccct
     8641 atacgcatcc catagcttgt atctaaaaca gatataacag ataaattctc actctttcct
     8701 tctcttaaaa gttcatcaaa ctgataagaa ctaagtatca ttaagtttgc tttaccttca
     8761 tttatagcgt ttagacaact ttctacactc ttataagatg ttgtcttagc attttcaaaa
     8821 ctcttttcta tataagaatc aatagactta taactactag ttaaagcaat atttaaatca
     8881 ttattgagat tggtatccaa ttttcctacc ataatcattg gaatatttat gtatggattt
     8941 gtcaatttca tattaaatcg ttttgcctta tcattttcat tgattgagcc acatagcata
     9001 gttgccttac cactttttat gtaatcaaga ctctcattat aattttttgt tttaatatat
     9061 tcaaatttta taccacattt ttttgatata attgacatta aatcggaact tataccttta
     9121 aatgaattgc tttctttatc ataatactct acaggagacc actctgaatc atataccata
     9181 gtaagttttg ggtttgcttt aataaaattc atttcttgcc gagtaaatgc tatggaatta
     9241 ttgacctctc ttttgaaata ttttttatat aattccaact cataatacat atcatttatt
     9301 tttaattctt ttaaagcata atctaattct tttaccaaat taggtttatc ttttgcagta
     9361 gcaaaataaa aaggttctac tgaaaatttg ctaactattt tcattccttt agcatttata
     9421 atacttccac aaacaaatgc atctgccttt ttttcattga cggactcaat taattcatca
     9481 atagaattac agtatatata ttcaactgaa aaattatttt cttctctata cttctcaaat
     9541 gcagtattat gaaagtttcc tctaagtaca gctactctca ttcccttaaa ggaacttata
     9601 tcttcataaa acatattatt gttactatcc tttgtatata gtgcactata ttcatatcct
     9661 gaactcagtt taggaaaatt aaatagttta tttctttcat cattcttctg taaaggtccg
     9721 agtaagtcta tttcaccctt ttctagtctg tctaaacatt cttcccaaga cccttttaca
     9781 aattcatatt cccatcttgt atattttgag atttctctta gataatcata accatatcct
     9841 gtataagaat gctcttttga tgaggatatt attccgtagt catcacaata tccaactctt
     9901 attttttttg aattttcaga ctcatcagca aagcatagta tattgttaaa acatgatata
     9961 ataagtaaaa atatcaaaga aagtatcaag aagtgctttt taaatgcttt catatttaaa
    10021 aacctccaat ttgccaactt actttaactt atttcgtttt ctagttgtaa ctacttttat
    10081 tgtaatggta aatatatcaa attgcaatat tctcatataa tattcttatt caactataat
    10141 gaatttatta ttatctattt aaatacacta tctaatatta gtttcttata agtcataaaa
    10201 gcacaactaa cactataatt ctagtgatta attttatttc aaaaaatata ataacactac
    10261 taatatttta aatatcaata gtgtcataaa aacttttcag attaattaaa tctttctata
    10321 tattttataa taatctaaat catgctaaat atttagacag aaaacttttt tctttaatag
    10381 taaatacttt tgtttgaata tcagaataat tatttgaata tctatctata aaaccatgat
    10441 ttgcatctaa aatctcaatt tggatatttt gtttcctaga aagttggtaa gaaatagaat
    10501 caacatcaaa tgaatcgtgc tttgcaaata ctaataaggt aggacatttt ggaattacat
    10561 ccatgtaatc tctaatccga gagccataac aacaaattat gccatcacaa aaagaatctt
    10621 cactgcatct ccatgcaata gttgctccca cgctataccc agcaacaaat acatatttat
    10681 actctgattt taaatcattt ataagctttt caactttttt ataaatatca aaaccaacat
    10741 tatttataaa aaaatcatat gcctcttgga cttttgaata tggaaaaact cttttttctc
    10801 ccagaagttc aggacaaaat acatcatatc catatttata atacttttga caaacatctt
    10861 ctataaagct attaatccca tagatttcat gcaaaacaac aatagctaca tcacttttat
    10921 gtatatactt tatcagatta tccaccacct ttacaaaaca gcaaattttt tatacttagt
    10981 ttaatatatt cttctttaca tgtcaattta cttttctaaa atcaaattta tttttggatt
    11041 aatcatttat ttttaaaagt tgtgcattat tactattaaa ttattctcta ttattaaaaa
    11101 tataatcgtt agaaataaaa aataaagtac aaataattaa aaaatatctc actcataact
    11161 ttaaaaatta taagtgagat actcctaaaa tctactttat attaaattta ttttccaaaa
    11221 tatctattta ataatcctaa aaatgcttta ccatgtctag cttcatcttt acacatttca
    11281 tgtactgtat catgtatagc atctaatcct aattcttttg ctttcttagc taattttaat
    11341 tttccatcag ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct
    11401 gcaactacaa cttctccaag aagttctgca aatttagcag catgttcagc ttcttcaaga
    11461 gctattctct tataagcttc tcctacttct ggataacctt ctctatcagc ttgtctactc
    11521 attgctaaat acattcctac ttctgtacac tcaccaacaa agttagctct taatccttcg
    11581 attatttctt catctacacc ttgagctact cctattctgt gctcatcagc ccaatttttt
    11641 tcgcctttca tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt
    11701 gcagcatctc cttcatgtat atatccacat actgtacaaa caaatttctt cataattact
    11761 ttcctcctaa attttatata tcaaattaaa ttttttattt attaagttag tctatatata
    11821 cccgcaccat atacctttaa acaatttttt taaaagtttt ttctaaatac ttattttccc
    11881 tgtgtcaata atatgtttct ataataatgt ccctttaatt ttatcaattc tttatgattt
    11941 cctgattcca caattctacc attttctaaa actattatat tatcacaatt tgatatagta
    12001 cttaatctat gagctataat tatacttgtc tttccataag ttaaattatt tattgcttct
    12061 tgaattttag cttctgttgc tatatctatg ttacttgtag cctcatccaa aataagtatt
    12121 ggagcatttt ttaatactgc tctagcaata cttattaatt gtttttgacc ttcacttaaa
    12181 tcttcacccc tatccgataa tacagtacta tatccttcac acattttaac tataaattta
    12241 tgtgcatttg ctattttact agcatatata acttcttcat cagtagcctc cagatttcca
    12301 taccttatat tttccataac tgtatcattg aatatataac cttcttgtgg aactactcca
    12361 aaacaagttc tcaaatagtt tctgttaata gagtatatat ccttaccatc taaaaatata
    12421 cttccttcat ctatatgata aaaatttgta attagattta taatagttgt ttttccactt
    12481 cccgtctctc ctatgatggc atttgatgtg ccagatttga tttttaggtt tatattatct
    12541 aaaattttct tttttgaatc ataagaaaaa cttacatctt taagctcaat ttctcccttt
    12601 acaaaatgag agtttaaatt accatcttta ttatcgttaa tctcactttt aaacatgtta
    12661 tgcacttctt gctcttcttg ttcatctata atttcaaaaa ctctttcaca tccagctatg
    12721 gctgaaagta atgtattaaa tagatttgct aattcattta aaggtcttgt gaattgtctt
    12781 acataactta aaaaactagc tataacccct atggttataa tatctttaga tgcaagaatg
    12841 cctcctataa tacatatagt tgcaaatcct atattactaa tcacattcat aataggcatt
    12901 aaaagagaag tgtaaatttg cgatttaagg gaaatattta gaagtctatc attgacttct
    12961 ttaaaatcat ttacagattt tctttcataa ttaaaagatt ttactacatt tatgtttgat
    13021 atcatttctt caatatgtgc atttagtttt cccaactcaa cttgttgttg cttaaaaaaa
    13081 actctagttc tctttgcaat aaaattactc acaaacatta ctatcggtat taatattaca
    13141 ctgcaaaagg ttaaaattgg gcttaataca aacatcataa taaaagtcat caatatgttt
    13201 aagattcctg taattaactg tactaatgaa tttgatattc ctgtactgat attatcgaca
    13261 tcatttgtca ctctactcat aatatctcca gtagaacttt tatcaaaaaa ggttattggt
    13321 agatttttaa attttaaaaa taaattactt ctcatttctc tgactatact ttgagataat
    13381 ctagctgtac atatcccctt agacagttta actgaaaact caattatata aaatgaaagt
    13441 aaaataatta ttccttttaa gaaaatggat tttgaataag tatcgatatc tattatgtca
    13501 actacctttc caatagtata tggtattaca gttgatataa ttgaatcaat tattactagc
    13561 aaaaaaataa atctagtaat cttcttatct tttttaaagt acataaaaat tcttttaaac
    13621 gtttttttaa aatctttagg cttttctttt ttctttaatc tactgacctt ctgatttcca
    13681 gtacttaaat tcaatatact tcctccttaa tttacatctt cacttactaa attctgcaac
    13741 gcaaaaattt ttttataaat tggagaacta accagtaaat cttcatgtgt agcaaatcca
    13801 accacctcac cctcatccaa gactaaaatc ttatcacagt caattataga actaatcttt
    13861 tgtgaaatag ttattacagt acatttctta ctttcctttt ctcttaaatt acttatatac
    13921 tcttttatat tctttcttat aattgtctct gtattaatat ccattgcact aaaacaatca
    13981 tcaagaatta atatttgtgg ttttttggct atagctctag ctatagtaat tctttgtctc
    14041 tgtcctcctg ataaattttt acccctttgg aataaaactt catcataagt attttcgagt
    14101 tttgttataa attgatttgc atttgctatt tcacatgatt ttacaacatc ctctttgcat
    14161 atatctctat ttcccataac tacattctcc aatatacttt ttccaaacac actactattt
    14221 tgaaatacta cacctattct tgcccttaaa ctttcttcag aaatactgta tatactacgt
    14281 cctccaatct taatatcacc tttatcaatt atatataatt tttgaattaa atttactaaa
    14341 gaagtctttc ctgaaccagt ttgaccaatc acaccaatat tttgaccttc ttctatttta
    14401 aaggatacgt tttttaatat tttttcatct gtatttttgt tatacgaaaa atctacattt
    14461 tcaaaaatta tatcacagtt tacaaacttt aaatcttttt ctccaacatc taaactttct
    14521 tctctctcaa ttacttcttc attctcatca accaaaacct cacttattct ctcataagat
    14581 gcttttgccc taacaaaaac attaaaaaca tgtgaaatca ttataagaga tgttaatatt
    14641 ctagtcatat agtttacata agcaactata atcccaattt taatttctcc tgaataaatt
    14701 aaatgccttc caattagtaa tattattatt atggaaacat taatgattat tgtaattgtc
    14761 ggtctaaaaa atgacatcat agttgtagct tttctactat ttttaaaaag ttcatcattt
    14821 aactcttcaa agctttggtt ctcataatcg tatctacaaa atagtttaac tacttttatt
    14881 ccacttaaaa actgtcttaa ttttgaattt actttatctg tactttcttg tacattttta
    14941 aataatctat accctatctt catattaagc aatattataa taaatataat taaaactgca
    15001 attagaataa ttattgacat ctttaaattt aattttatag ctataattaa acttcctata
    15061 cacattattg gtgactttat aaaaactctc atcattccat taacgaattg ttgcatttga
    15121 ttgatatcat tagtaattct tgttattaat gaagctctac caaatttatc tatttgtttt
    15181 aatgagtatg tatttatctt ttcaaataca tcctgtctta aatcataaga aaaatgctga
    15241 gaaactcttg ccgagattac acttcttatt attgcaaata ttgcacctaa aaaagttata
    15301 cctatcatta tagctcctgt tgatagtatg tagtctaagt tcttatttga aacccctata
    15361 tctattattt ttgccattat agttggttga gctaaatcac aaaaagcttc caatgttaaa
    15421 aaaaatactg ctgttagaaa taatactata tattttttta tgtatttttt aaaatacctc
    15481 attcaatcat cccttcatag aattttctca ttaccaaaag tatatcataa tggctaattt
    15541 aattgattat atcaattata aatatttgat ttattattaa tttttatacc atatatttta
    15601 taaattattt gtttttattt cctatattta tatat
//
LOCUS       DAFKMX010000099        15632 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFKMX010000099 DAFKMX010000000
VERSION     DAFKMX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.099"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            301..1185
                     /locus_tag="LZB08_002341"
     CDS             301..1185
                     /locus_tag="LZB08_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7315838.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            1387..2937
                     /locus_tag="LZB08_002342"
     CDS             1387..2937
                     /locus_tag="LZB08_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBM7315839.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            2966..3613
                     /locus_tag="LZB08_002343"
     CDS             2966..3613
                     /locus_tag="LZB08_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBM7315840.1"
                     /translation="MRKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDINYLNISEL
                     SGLSKADIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(3755..4015)
                     /locus_tag="LZB08_002344"
     CDS             complement(3755..4015)
                     /locus_tag="LZB08_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBM7315841.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            4232..5515
                     /gene="hflX"
                     /locus_tag="LZB08_002345"
     CDS             4232..5515
                     /gene="hflX"
                     /locus_tag="LZB08_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBM7315842.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFLSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            5523..6200
                     /locus_tag="LZB08_002346"
     CDS             5523..6200
                     /locus_tag="LZB08_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBM7315843.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            6286..6729
                     /locus_tag="LZB08_002347"
     CDS             6286..6729
                     /locus_tag="LZB08_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBM7315844.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            7034..7678
                     /locus_tag="LZB08_002348"
     CDS             7034..7678
                     /locus_tag="LZB08_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBM7315845.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGTKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(7764..8141)
                     /locus_tag="LZB08_002349"
     CDS             complement(7764..8141)
                     /locus_tag="LZB08_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBM7315846.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(8141..8527)
                     /locus_tag="LZB08_002350"
     CDS             complement(8141..8527)
                     /locus_tag="LZB08_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBM7315847.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            8706..9461
                     /gene="nadE"
                     /locus_tag="LZB08_002351"
     CDS             8706..9461
                     /gene="nadE"
                     /locus_tag="LZB08_002351"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBM7315848.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            9544..10263
                     /locus_tag="LZB08_002352"
     CDS             9544..10263
                     /locus_tag="LZB08_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBM7315849.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(10430..11434)
                     /locus_tag="LZB08_002353"
     CDS             complement(10430..11434)
                     /locus_tag="LZB08_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBM7315850.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            11700..12596
                     /locus_tag="LZB08_002354"
     CDS             11700..12596
                     /locus_tag="LZB08_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBM7315851.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            12717..13979
                     /locus_tag="LZB08_002355"
     CDS             12717..13979
                     /locus_tag="LZB08_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315852.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            14055..15248
                     /locus_tag="LZB08_002356"
     CDS             14055..15248
                     /locus_tag="LZB08_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBM7315853.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
BASE COUNT         6017 a         1784 c         2853 g         4978 t
ORIGIN      
        1 taaattttga cttagctaag ttggtttttt tacttaaagt attattttta aaatgaaaat
       61 ttttaagttg cataaaacac ctctttctta ttaaattatt ttaataatag aattgaagtt
      121 ttcttgctgc ttctatatta tgttgtgcaa gaatcatgtt ttgatgcttg gaaaactagc
      181 tcaataacac cttagttttc ataaaaataa ataaaataaa tagtgtatat gataaaataa
      241 atacatattc atataaataa aaataaacaa taactgtcta gcataagaaa ggagcaaaaa
      301 atgaaaaaat atagattgtt agttacttgt ataattgcca tgggaatcat aattatagga
      361 acatcaaatc cagttagcaa tttttacaag actacaaatt tgattagttc aggaaatatc
      421 acaaatgaaa aagcagataa agatgaaaat tcaaacaaaa ataataaaga taaaaatgaa
      481 gacaaatcta attctaaaag taataaagat aagtcatcag aggacaaatc gtcagataat
      541 aaaaatgaaa ttcaaaaaaa taaaaaactt ttaatttgta tagacccagg gcatcaagga
      601 aaaggagata gcaatttaga accagtagct ccaggttctt cctctaaaaa agcgagagta
      661 tcttcaggaa cagagggtat tgctacaaaa aaaccagaat atgttttaaa cttagaagca
      721 tctcttgttt taaagagtat attagaatct aaaggttata atgtaataat gactagagag
      781 acacatgatg taaatataag taattcagaa agagcaatac ttgctaatga caaaaaagca
      841 gatatggttg taagaataca tgcagatagt ttaaataatt cttcaaaaac tggagcatct
      901 atattgattc ctgaaaaaga tggaaaatat acagctccta tatatgaaga aagtaataaa
      961 tgtgctgaat ttatcaaaca gaatatggaa caatcaggta tacaaatcaa tggaatcgtt
     1021 caaagaggtg atttgactgg attcaactgg tcaaaagtac cagctgtatt agtagaaatg
     1081 ggatttatga gtaactataa tgaagaccaa atgatgtcaa atccagacta ccaaagaaaa
     1141 atgatgcagt gtatagctga tggattggat gcgtatttta aataagatat gaatagagaa
     1201 aaagttaggt tggtattatt tgcctaattt ttatctctat tttttattgt acataacaca
     1261 aagaagaagt ttattttagg ttaaattgtc gtaaatataa ctaaaaatat tgggaatact
     1321 tgatttaagt ttaaaaagaa tatattatag tatagaatgt gtaaaatttt tttgagaggt
     1381 gttgaaatgc tagtagtaga aaatgtgagt catggatttg gtgcgagaac aatattagaa
     1441 aatgtatcct ttagacttag aaaaggtgaa catattgcac tagttggtgc taatggagaa
     1501 ggaaaatcga gttttttaaa tataataaca aagaagctta tgccagatgc tggaaatata
     1561 aaatggtctt caagagctac agtaggttac ttagaccaac atacggtatt aagcaaggga
     1621 aaaactataa gagaagtgct tagagaggct tttaagcata tgtttgactt agagcaagaa
     1681 atgattgcta tgtatgacaa aatgggagaa gctagtgatg atgaaatgag taagcttctt
     1741 gaagaaacag cagagataca aactatactt gaaaatagtg gtttttatat gatagatgca
     1801 aagatacaag aagttgcaaa tggtcttggt cttggagaaa ttggtcttga taaagatgta
     1861 actgacttaa gtggagggca aagaacaaaa gtacttctta ctaaacttct acttgaaaat
     1921 ccaactattc ttatattgga tgagcctaca aactatttag acgaagagca tataacatgg
     1981 cttactaagt accttcaaga gtatgagaat agttttgtct tagtttctca tgatattgaa
     2041 tttataaata atacatgtaa tgtaatatat catatggaaa atggagagtt aaatagatat
     2101 aaaggaaatt atgatgaatt tgtaagactt aatgatataa agaaacgtca agaagaacaa
     2161 gcttatgata aacaagttga agaaagaaaa agactagagg attttgtagc tagaaataaa
     2221 gctagagtag caactagagg aatggctaac agtagacaaa agcaacttga taagatggaa
     2281 atattagaaa gaccaaaaga aaaaataaaa cctacttttg cttttaaaga tgctagagca
     2341 gctagtaaaa taatttttga aacagaaaat ttagttttag ggtatgatga agcattgaca
     2401 aaaccactta atttccattt agaaagagga aaaaagattg ccttaaaagg aatgaatggt
     2461 attggaaaat ctactctatt aaaaacttta cttggaataa taaaaccatt tgaaggaaat
     2521 gttaaattag gtgactattt ggaagttgga tattttgaac aagagagttc aagagaaaat
     2581 agtaatacac ctatggatga agtttggagt gaatttccag ggcttacaaa ctttgaagtt
     2641 agacaggctc ttgcaaaatg tggacttaca aatgaacata taacgagtca aatgagagta
     2701 ttaagtggtg gagaagctgc taaggttaga ctttgcaaag taatgttaaa aaatataaat
     2761 ttcttagtat tagatgagcc tacaaatcat ttagatgttg aggcaaaaga tgaattgaag
     2821 aaggctatca aagagtttaa gggaacagtt cttttagtat gtcatgagcc agaattttat
     2881 tcagaaattg ttgatgatgt atggaatata gaagatttta caactaaaat cgtgtagtga
     2941 tttaacttat tgctgggggg atttcatgag aaagtttcta aatgtacttt tttcaatttg
     3001 tattagtatt gttataatag taggtgttat taattttact gttggattta aacaacttta
     3061 ttattttgac attaattact taaatatatc agagttatct ggtttatcta aagctgatat
     3121 aaaattgaac tacgattatc taattgatta taacttaaat aagaatgtgt ctgagttcaa
     3181 attaccaaca ttaaaatctt ctccacaagg gaagatacat tttgaagaag tcaggaatat
     3241 atttcaaaat ataaataaat tagctaaatt attattggta gtttctttag taggaattat
     3301 attgagtgta aaaaataaaa atataaaaat cttaaaaact acatcaataa cacttataat
     3361 tatgccatta ttattaacag ttccaatttt attaaatttt gaaaagtcat ttattatttt
     3421 tcataaatta ttatttagaa atgattattg gatatttaat cccgatttag acccagttat
     3481 aaatatattg ccagaggaat ttttttttca ctctggaatg atgattttaa tattaatttt
     3541 gttagtaagt atagtactgt ttgtaatgta taaattatgt aaatctttat caataaaaat
     3601 atttcaatgt taaatagagg gatattttta tttccaaaat aagataataa aaaaggatta
     3661 gattaaaagt tatacaataa aaatagctgt gcaataatat ttagactatg aaagtagtta
     3721 aaaaatattt atgcacagct atttataata gaatttactt tttactagtg attttaacta
     3781 aatcttgaaa tttactcata atgtaattgt aattatcttt tttatgaggt aatatacaac
     3841 tactgcaatc tttgatacct ttatctgtat acttaaagtt tccaccacag ttttctccta
     3901 aagcatacaa aggacaatag caaaatagac agttaaactc ttctggttta ttagttttat
     3961 gacaagggaa aaattcgcaa tctttgtgat taaaaaactt ataattttcg cccatatttt
     4021 cctcctaatt aaataaaatt taaatttttt atactatata agattttggt gataatataa
     4081 ttatactaaa taattttatg aatagtcgat aatatagtat tattaatcat agtattaatg
     4141 gtataattag tcaacagaaa aataatgtaa aaataatgta agtgtatttt gtaattagaa
     4201 tatgttttat aacaaaaaat gaggtgatta agtgaaagaa attcaagaaa aagcacttct
     4261 tgtaggtctg aatttgacta caatagtgaa aaaaaatgat gatatagaca ctaatgaatc
     4321 tatggaagaa ttaaaagaac taacaaaagc tgctggggca gaagttgtag gtagcttaat
     4381 tcaaaataaa cactctgtag atgcagctta ttatatagga aaaggtaaag tagaagaaat
     4441 aagagcctat agtgactctt tagatgctac tttagtaatt tttaatgatg aattatctgg
     4501 tgctcaaatc agaaatatag agaatgttgt tggaagaaaa gtaatagata gaactacttt
     4561 aattttagat atatttgctc aaagggcact tagcaaagaa ggtaaattac aagttgaatt
     4621 agcacaatta aaatatagat tgccacgact ttatggaatg ggtggtgaga tgagtagaac
     4681 aggtgcaggg attggtacta gaggtcctgg agaacaaaaa ctagaaatag ataagagaca
     4741 tatacttaat aaagcagcag atataagacg tgaacttaaa gaagtaaaaa agaatagaga
     4801 gacacagaga gttaagcgtt taaagtcaaa tataccgatt gtagcacttg ttgggtatac
     4861 aaatgcagga aaatctaccc tgttgaacga attaataaag acacacaagg attatgaaca
     4921 agaaaaagaa gtttttgtaa aagatatgct ttttgcaact ttagatgtaa ctttgagaaa
     4981 ggctcttttg ccgaataaaa aagaattttt agtagtagat actgttggat ttttaagtaa
     5041 acttccccat gatttagtag aagcgtttaa ggcaacattg gaagaagtac agtatgcaga
     5101 tttaatctta catgtaattg atgcaacaaa tactagttat gaattgcaaa aaagtacgac
     5161 tgaaggtgta ttaaaagaac ttggtgtaaa tgataaaaaa catatacttg tatataataa
     5221 agtggacaag ctagaattgg atatatatcc taaaagtcaa gaagatattg tgtatatttc
     5281 tgcaaaacaa ggcataaata tggataagtt attaaatatg atagaaattg ctttaatgga
     5341 aaatacttat tcagtaagtt tgatgttacc ttatgagaga ggtgatatat ttagtagaat
     5401 aaaagataaa tataatgtgg agaattttga gtatggggaa agtggtataa cgctagatgt
     5461 agatttagat gaagaagatt ttaatatcta tagagagtac atactagaaa aataggttga
     5521 atatggatat aaactgggaa aatatagata atcttgaaga ttattttata acctatcttc
     5581 tttataaaga atctaaaaca gtatctcaaa ttagtaaaat tagaaatatt tcaagtactg
     5641 aagcaagtga gcaacttata caagcaaagt taaaaataaa ggaaatgcaa aaagatgatt
     5701 ttgaggcatc taaggatatt ttggataaat ttctagaact agataaaatt aagagacttg
     5761 attttatgga tagcttagat gacgaaaaaa tggtatactt taagagaaag gttttcaaaa
     5821 gaatccttgt agaaaaaaat gctgaagatt taattgtttt aatttgggcg acaggtgagc
     5881 taaaagatga taggtttttg aaattgcttc atcagctcac aaaccacaga cactcagata
     5941 ttagaagaat aacttattca gcaatcagaa agattgaatc accaagtagt agagaagtac
     6001 tgcaaaaagg tctctacgac aaaaatgcac agactaggca gtactgtgca aaagctcttt
     6061 caaaattggg agatgaaaac agtttaaaga ttttacagca gttgaaagac aagaataaga
     6121 attttgaaaa agaatatgtg cttagagctt atgacgaagc cattaagtca ttggaaaatg
     6181 caaaaaattt gaaaaagtaa ttttttatta aagcaatttg tttacttaat gatgtatttc
     6241 taaagtttaa caaggtaata aataaattaa tgggaggggt ttcaaatgat agtaagacgt
     6301 tgtgcaggtg gagtagtgtt ttatgctaat aaagtactta ttgtaaaaaa tgataggggt
     6361 gaatggacac ttccaaaagg aaagatacta ggagggggac ttccatatga gagtgctgtt
     6421 caaagagtta aagtagaaac tggcatagat gctaaaatga tagatgtggc tggcgatact
     6481 atgtatgaat ttttctcaag aagtagacaa caagaagttt gtaatgcaat aatgtggtat
     6541 gttatggaag cttgtaacac tgattgtgta cttgctcctg aatttcaaga aggtggattt
     6601 tataaagtaa aggatgcttt agaaatgtta tcacatcata aagagcaagc tttagtagaa
     6661 gtatcttata aaaagtttaa ggaattaaag aaattagttt cagagggcga tgatgttcca
     6721 gcaaattaat tgtgaattta gaggattctg aggaagggat agccccttcc ttttttaatt
     6781 gaagcagttt taagcgtaaa ttatatagtc tctttaaact atgatttatc aatttagaga
     6841 tttagtagtt tatatttaaa atcagtaaga tatatttaaa ataaaaatta aaaagtatac
     6901 aattttatta tataattgtc ttatgatgtt gattaaagaa ttattaaaag tccatttatt
     6961 tttaaataga ataattttat aaatatatat ttgatgtgta tattagaaaa tgattaaaat
     7021 aggaggaaat acaatgagca attacaggac attacatgaa tttggaacgg atgaaataac
     7081 gattgaaaag tctgttttta ttggatatgc taagcctata aagagtgaag aagaagcatt
     7141 agagtttata aatgaaataa agaaaaagca taaagatgca actcacaatg tgtgggctta
     7201 tactgtaggt aaaaatatga atatacaaag atatagtgat gatggagaac cacaaggaac
     7261 agctgggata cctacattag aggttataaa aaaagaagat ttgagagatg tagtggttgt
     7321 tgttactagg tattttggag gaacaaaatt aggtgcaggt ggtctagtta gagcttatac
     7381 aaaaggtact aaactgggac ttgaagcagg taagataatt tataaggtaa tgtatcaaga
     7441 agttaaggtt aagatagatt atactcaact tggaaaagtg caaaatgagt taatgaattt
     7501 ggggtatttt ataaaagata ctgtatatga ggataatgtt gaaatagtag tatattcaag
     7561 attagaagat gtagagaaat tatcagaaaa aatgattgat ataacaagtg gaacaggaaa
     7621 aatagtttta ggagaggaat tttatttgtc tgaacaaaat ggagaaattt taatataatc
     7681 taaaaggtta ttagaatagt taatacctca aatagccaat tagtattttt aagtgaaaag
     7741 ccattaaatt gatttaatct aaatcaattt aatggctttt tatcggattt tgcacagaat
     7801 ccttttttta cttcaaataa accaactaaa agtaaaaata tcccaaatat tttttttaat
     7861 atatctgaag acagataatt agctaatagt gaaccaaata cagctcctaa aaccccaaac
     7921 acagctatag gtataaccaa tttaaaaatt acatttttat tttttatatg tatagcaagt
     7981 gctataagtg tcatagggat agatgaaagt aaatttacgc tttgtgctat tttagtgtct
     8041 atgccagcaa gtaaaacgag tgcaggtata aggattgttc ctccacccat tcccattcct
     8101 ccaataattc ctgcaaaaaa tccaattaga gcaaataaca ttaaaacacc atccttaatg
     8161 ctgcaattat cataattatt ccaaaagaaa ttctaagaaa ctttccagta accttattta
     8221 gtaatttagc tcctataata ccaccaagaa tacttccaac tgcaacttta attgttaagt
     8281 taacatcata agtaccttta gatacatata aaacagaact tgctgtcgtt aagaatatta
     8341 ttatagaaag agcagttgca tgagatttat gttcttctac cttaacgatg tcatttaata
     8401 ttggaactaa aagagtacct cctccagaac caaaaactcc atttataaat cctgtaaaaa
     8461 ctccaattat cgtatttttt aagttaaaat caaagtattt tttctttaaa tcatcagttt
     8521 ttttcataaa aatcaccttc tgaaatagtt ttaccaaaat aggcaaatta tatttatata
     8581 aaataagatt aaatataatc tttcatgttt gtttaatttt gcataaaaaa aaacatatta
     8641 aaacaacata ttaatatatg gtataatata ttaaaacaaa gtagcaaatt tgagaggaga
     8701 taacaatgtc aaatataaaa atacaaatag acaaaactgt agaatggctt ataaataagg
     8761 ttaatgaggc aaatgcaaaa ggattaattg ttggtgtatc tggaggaatt gattcggcag
     8821 tagttgctaa tttaattaaa aaggcatttc cagaaaactc tatgggagtt ataatgagca
     8881 taaaaagtaa tcctcaagat agagaagatg cacttaaggt tatagaggga tgtgatatag
     8941 aataccttga tttagacctt atagaaccac aaagtgctat attagacatg gtagtaggaa
     9001 atttaaaaga taaacattta tatagagaag aatatttaaa gatgacagat gcaaacttaa
     9061 gagcaagagt aagaatgagt acaatttata caattgctaa caatctagga tatctggttg
     9121 ttggtacaga caatgctgca gaaatacaca caggatattt tactaaattt ggtgatggtg
     9181 gagttgatat tttacctatt gcaaatttaa ctaaaggaga ggtgtatgaa tgggcaaaag
     9241 agcttggtgt tcatgaagat ttaataaaca aagctccttc agcaggtctt tgggaaggtc
     9301 aaacagatga agatgaaatg ggaactactt acaatatgat agatgctgtg ttagaaggta
     9361 gattggatga agttcctaaa agagaccaag agataattga aagacttcat agattaagtg
     9421 aacacaagag aaaaacgcct gcacaacctc ctaagttcta atttactatt tataagattt
     9481 gtgataaaat ttacaagtga tgaaaattag aataattaat tgtacgtatt aaggagtgga
     9541 aatatgggac gtataggtaa tataattaat agaaaaggta aacaagatgc tcaaagagca
     9601 aagatattta ctaaacatgc tagagcaata gcagttgcag ctaaagaagg tggagcagac
     9661 ccagagtaca atgcagcgtt aaaaacagct atagaaaaag ctaaggcaga taatatgcca
     9721 aatgataata tagatagagc tatcgctaaa ggtgctggag caggtgctgg agaagactat
     9781 gaaactatag tttatgaagg atatggacct ggtggagttg ctgttatagt agaaacttta
     9841 actgacaaca aaaacagaac tgctggtaac gtaagatatt attttgacaa aaacggagga
     9901 aacttaggaa ctagtggatg tgtgtctttt atgtttgata aaaaaggaca aatattagtt
     9961 ggtttaggag atggagtttc agaagaagaa ttgatggatg ttgcattaga agcaggagct
    10021 gaagatttca taacagaaga agatggttat gaaataataa ctacaccaga agatttttct
    10081 agtgttcgtg atgagttaaa agctaaagga tatgagttta tatctgctga tgtgaagatg
    10141 atacctcaaa ctacaactgt attgactgaa gaatctcatt taaaaatgat gaataaatta
    10201 gttgatatgc ttgaagaaga tgatgatgtt caagatatat atcataactg ggaagtagaa
    10261 taaatagtat aagcatattg aaatttataa gtattgaatc aattttaaca tgtgataaat
    10321 tttatagaat aagaaacact aaaatagaca tacgcacctc taacctatat ttgcataata
    10381 atatgtaagc ataggtaagg ggtgttttta tatttatagg ttaagaaatt taaagtaaat
    10441 tttgagctgg actcgattgt ctataaataa ttgatgggta tataacttgt tgctgaatac
    10501 catttatacg cccagttact gtatcaataa tcatcctaag actacgttct gctgtctctt
    10561 ccatttttaa atctataact gtcaaaggag gaatacttct tgccattagt cgtgaaggtc
    10621 cattacttgc tgcaaatatt tgaatatcct ctccaattac aattcccgat tcatgaaatg
    10681 cattcatcat accaagagct accacatcat gattacaaaa aattactttt tttagtgaat
    10741 tttttgcaag cattttttga gcaatatcat atccactatc tattttatta gaacaataat
    10801 atatagaatc tgatagatgt attccatgtt ctttacatgt ttttaaaaaa gatttacccc
    10861 ttaaagtcat acatgtaaag gaagttgggt tgaatacaac agcaatatca tttttaccat
    10921 tttgaatagc atgtaaagct gctatacgac cagtttcctc attatcaata gatacacttg
    10981 aaaaaccagg tatttcacga tttacaacaa ctacaggaat tttaggttta ttttttacta
    11041 gatattctat atcatcagca ttgatacctg ttaaaacaat tccatcataa taattatttg
    11101 caaaaaagta ttcatcatca catagatgac caactttgta aggtcgtatg atgatattta
    11161 tgtttaaatg ttctaaagaa attatgtggt ttattgcttg aagtatattg gttgatgcca
    11221 actcaaatcc ctcatcctgc caataaaaag ctattttaat attattatgt ttaataggag
    11281 tagagcgttt atatccaagc ttatcagctg aatttaatat aagctcttgt gtatgtagcg
    11341 gaatcctgcg ttcatttcct ttaccactta agacgtacga gacagttgct gaagaaagac
    11401 cacagtcttt tgcaatatct ttgattgttg ccatgataaa atcctcctta aatagttgct
    11461 taaatttgct tataccaata atcttaacat atattcaact aaagaacaat attctacaag
    11521 gtgcatattt tacaggatta gaataaataa attaaaaaaa acttcttact tatgttgaca
    11581 taatttagtt tataggatta taatatgctt aaataagctt atgattaact taaattaagc
    11641 aaagttaagc aggaacatgc aatgctagct tgcaatgtga ctgtaaaggg ggaaaaggta
    11701 tgagtaaggt aaatatattt gaagttggtc ctagagatgg atttcaaaac ctgaaagagt
    11761 ttttagaaac tgataaaaag cttaagatta ttgatggtct tattgcttca gggattaaac
    11821 aacttgaaat tagttcattt gtaagcccaa aagcaatacc tcagatgaaa gatgcaaaag
    11881 aagtagcaac ttattgtgta gaaaaatatc ctgatgtgaa actttatgca ttagttccaa
    11941 atttatacgg tgctaaagtt gcatgggagt gtggtataag agatatatcc tatgttatat
    12001 ctttgagtga aactcataat aaaaaaaata taaatcgtac ccatgaagag tctattgatg
    12061 agcttaaaaa aataatgaat acttatccag acatgaatat atgtattgga atggctactg
    12121 tatttggatg tccttttgaa ggtttaccaa aaattgaaga tgccataaga tttgctaaac
    12181 gcctttggga aaacggtatc agggagataa atctagcaga tacaataggg attgctgacc
    12241 ctaagcaagt aagagaaact attagtgcat tagttgaagc tttaccagaa tgcagatggc
    12301 aagtacatat tcatgatact agaaatatgg gaatggttaa tactcttgtg gctattgaat
    12361 ctggaattac tactgttcag tctacacttg gaggattagg aggatgccca tttgctcctg
    12421 gagcatctgg caatactgca actgaagatt tagtatatat gcttgaacgt atggggtatg
    12481 atacaggagt tgattttgtt aaacttcttg aattatcaaa gtatcagaag agttttgtaa
    12541 atggagtata tagtggtcat catgtaaata taaagtctga tagttgtaat tgttagttct
    12601 attgattaat cgattaaaaa aatatgctga ttggtaaaat agatttattg ataaaaaata
    12661 taaattataa aatacattat ctaataaaac aaataaaaaa attgaggggg attagtatgg
    12721 atattatagc tttactttta tttttaggag ttattattat tgcatttgta cgtaagaata
    12781 atgcaggtat tttggctcta gctattggtg tcatagctgt tagaattttt ggaatggatg
    12841 ataaagaact ttatggagct ataagcaact caatgtttac tacacttgtt ggtataacac
    12901 tactctttgc aatagtgaac tcaacaggag cattaaagct tgttgcagaa aaaattgtag
    12961 ctgcttctgg taaacgtgtt tggcttatac ctatagcagt ttttatagca ggatttgttg
    13021 ttgctggtgt tggacctggg gcaattccag cacttgcaat tatcccagca atggctgttc
    13081 ctgtagccat aacagttggt tataatcctg taatgcttgc acttataggt gaatgtggat
    13141 taatggcagg tcgtatgact cctattactc cagaaggtca aattataaca accgttgcag
    13201 aaggggttgg aattgaaaat gttatgccta ctatcttagc ctgtcaaact ctttctacaa
    13261 ttgtattttc aatagtattg tttataattt tcaaaggata taaacttaaa aaacctataa
    13321 atgttataga tagtaagcaa atagaaaaat tcaatataaa gcagataatt tctctaataa
    13381 gcatagtagt tatgattgct tttatcataa tttttgatat gaatgttggg cttgcagctt
    13441 ttgcagtttc agctgtactt atttttttca atattgctga tgaaggagaa tgtatcaaat
    13501 caattccatg gagcacgata gtaatggttc ttggagtagg tgcaatgatg acaatcgtag
    13561 atgcagcagg aggtattgac ttgatgagta atgcattatc atcactgatg aatagtaaaa
    13621 cagctactcc aattatgagt atttcagcag gacttatgtc tatggttagt tctgctttag
    13681 cagtagttta tccaactatg atgcctatgt gtgttgatat agctaaggca gttggaggag
    13741 tagacccatt agcacttatt gctgcagttg gtgttggagg ttctcttgct ggtgtaagcc
    13801 cactttctac tggtggggca ttgattttag cagctatggg ttcaagtaag aaagacttta
    13861 ataaggaaga acaaaataag gtttttgtac agcttttcat tatgagtgca gttggcttat
    13921 tggttattgc agtagtatca atattatcat ttaatgcaat aacacattta atgaactaat
    13981 tagaataact gaaaataaat cattaaattt aacttttaat attaatcaat ataatataat
    14041 ttggagggga agttatgaaa aaaggagcat taagcaatat aaaggtactt gaccttacaa
    14101 gagttcttgc aggtccatat tgtacaatga tgttggcgga ttttggagct gatgttataa
    14161 aaatagagat gccaagaaaa ggtgatgata caagaggttt tggacctttt gtaaatggag
    14221 aaagtttata ctatgcaaat ataaatagaa ataaaaaagg tatcactcta aaccttaaat
    14281 cagaagaagg aaaaaatata tttttagaaa tggtaaaaca tgctgatgtt gtagtagaaa
    14341 attacagacc aggtgtaatg gaaaagttag gtctgggtta tgatgagttg gctaaagtta
    14401 acaatcaaat aatttatgga gctgtatcag gatttggatg ttatggacca tattcagaac
    14461 gtccaggtta tgatattatt gcacaggcaa tgggtggtct tatgagtata acagggcaag
    14521 aaggaggaga accaacgaga gttggtaatg ctatgggtga tgtactaggt ggaatgaatt
    14581 taactattgg tatactcaca gcactcaacg ctagaaaaat aataggaaaa ggacaaagag
    14641 tagatgtttc cctcgttgat tctgttgtat cgtctctcga aactggtata cagagatact
    14701 ttgattcagg acaacctgaa cttatgggta atagatatgc ttcagcctat ccttatgact
    14761 cttttaaggc aagtgatgga agttttgtaa taggctgtgg taatgacaaa ctttttgaaa
    14821 agttatgtaa aaaagttctt tttagagaag accttttaat agatgagaga tttaaaacaa
    14881 atcttaatcg ttgtgaacat tacaaggctc ttaaaccaga aatagaaaaa tggtctgtta
    14941 agcatacaat aaaagaagca gttgacttaa tatcagaggc tggagttcct gctgctccta
    15001 tctataatct tgattgtgtt acaacagatg aacatatagc aaaagcaaga gaaatgattg
    15061 tagatattag tcatcctatt ataggaaata tgaaagtcaa tggaaatcca gttaagttga
    15121 tgacaacaaa agctaaaatt gatatgccag caccatctct aggggaaagt aacgaggaga
    15181 tttataatgg ttggcttggt tttgatagtg ataaaataaa agatttaaaa gaaaatggag
    15241 ttatttgatt aaaaaataac taaacacact tgtgacttta gtcatgggag gttcagttag
    15301 aataatatca aaaaatatta aatcaatact gtaaataaaa tatagaattg tcagaagcat
    15361 ttaagatggg aaaagctact tagacattga gtctttgtgg cttttcctat tttcattaag
    15421 aattttatat tatcagaata gatatgtact aaactaaagt attaacaatt taaattaata
    15481 gaaaataaac tacctaatta tgttgaataa agactacttt agtgaaaata aacttataga
    15541 gatgatattt aaattatttt aatggaaata tcaaaaatga gactgcattc tcaaaaatga
    15601 gactgcgttt tttaagtatt ttctcgaaaa tg
//
LOCUS       DAFKMX010000100        15529 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFKMX010000100 DAFKMX010000000
VERSION     DAFKMX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.100"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            262..570
                     /locus_tag="LZB08_002357"
     CDS             262..570
                     /locus_tag="LZB08_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315854.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGLKLLKQVQELLSK
                     "
     gene            659..2005
                     /locus_tag="LZB08_002358"
     CDS             659..2005
                     /locus_tag="LZB08_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315855.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            2238..3335
                     /locus_tag="LZB08_002359"
     CDS             2238..3335
                     /locus_tag="LZB08_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBM7315856.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            3434..4348
                     /gene="murQ"
                     /locus_tag="LZB08_002360"
     CDS             3434..4348
                     /gene="murQ"
                     /locus_tag="LZB08_002360"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBM7315857.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            4458..5378
                     /locus_tag="LZB08_002361"
     CDS             4458..5378
                     /locus_tag="LZB08_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBM7315858.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            5395..6246
                     /locus_tag="LZB08_002362"
     CDS             5395..6246
                     /locus_tag="LZB08_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7315859.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            6858..7919
                     /locus_tag="LZB08_002363"
     CDS             6858..7919
                     /locus_tag="LZB08_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBM7315860.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(8035..9009)
                     /locus_tag="LZB08_002364"
     CDS             complement(8035..9009)
                     /locus_tag="LZB08_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315861.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            9465..10346
                     /locus_tag="LZB08_002365"
     CDS             9465..10346
                     /locus_tag="LZB08_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBM7315862.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRISNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            10728..11531
                     /locus_tag="LZB08_002366"
     CDS             10728..11531
                     /locus_tag="LZB08_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315863.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            11543..12673
                     /locus_tag="LZB08_002367"
     CDS             11543..12673
                     /locus_tag="LZB08_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7315864.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNENHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            12677..14086
                     /locus_tag="LZB08_002368"
     CDS             12677..14086
                     /locus_tag="LZB08_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315865.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            14297..14806
                     /locus_tag="LZB08_002369"
     CDS             14297..14806
                     /locus_tag="LZB08_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBM7315866.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         6192 a         1629 c         2737 g         4971 t
ORIGIN      
        1 aaaagtattg agactatttt atgttaataa taagatagtc ttttttcttt tttaagaaaa
       61 gatatgagta ttttaagtag tgtgtaattt tagacaaaag aatgattaga taaataaagc
      121 aattaaagca agatataaat tcatgaaata aaattacaaa taaaaatata aaatatgaaa
      181 aaatatatac aaacgtattc ctgtaatcaa aaatacatgc tataatgatt gaaaagaaat
      241 aaaaaaacag ggggaatcaa tatgagagta ttatttgttt gttcatcagg gatgtcaagt
      301 gctatagcag ttaatgcatt acaaaaagaa ggagcaaaaa atggaataga tatagatgtt
      361 ttggctgttg gtactcaaga gtttgaggat gaagttaaaa atggatggga tattgctatg
      421 gtagctccac aagtcagaca tagatttgac tatttaaaag cttttgctga tgaggttagt
      481 gtaccatgtg cattaataca agcacaagct tatagtccat taggaggact aaagttatta
      541 aaacaagttc aagagctgtt gagtaaatag tagttgcagt gttgttaggt tagaagaaag
      601 ttgattttat aaaaatttta atgataataa aaataaatat taattaagga gagaaaagat
      661 gctagataaa tttacagaat ttttagacac taaattatca acttctatgg ccagattggc
      721 agaacaacgt cacttaagag ctgtaagaga tggaatcgta gcttcattac cacttataat
      781 agttgggtca tttttcttga tattagcatt tcctccatta ccagattcat ggggggtaac
      841 tcagtatcta aaggaaaatg cagttacaat attactacca tatagaatga cattttttat
      901 tatatcagta tattctacat ttggcatagg ttatagtttg gcaaagtcat ataagttaga
      961 tggattgaca ggaggtttat tagctacagc tacattcctt ttgacaatag ttcctactgc
     1021 aatagagggt cttacccctg atttattaaa gatagttgaa aagagtccag aacttttagc
     1081 ttggtataat tcaattcctc aaggatggca aatgccaatg gctaatcttg gtggaggttc
     1141 aatgtttgtt gctattgtaa catctataat aggtgttgag atttatagat ttactgacaa
     1201 atctaaattc aagataacca tgccagaagc agttccagca tcagtcacaa ggtcttttga
     1261 agctctgaca ccagccgcat taataataat tttaatggct attgttactt atttcctagg
     1321 gtttgattgg catggcttca tagctaaatt agtaactcca ttagtttcag ccactgacac
     1381 attaccatca gtaattatac taatattttt aataacgttt ttttggtcat ttggaataca
     1441 tggtgtatca ataataggtt cattggcaag acctttatgg atacaattat tagatgctaa
     1501 tacagcttca tatgctgcgg gtcaagcagt tccaggtatt gctgctgaac ccttttttca
     1561 atggtttata tggattggag gttctggagc tacaataggc ttagctttac ttcttgcatt
     1621 tagagcaaaa tcagcttatg gaaagtcttt aggaaaagca actgtaatac cagcaatatt
     1681 caatataaat gaaccaataa tatttggagc accaatagta ttaaacccaa tattaataat
     1741 accatttgtt gtggcaccaa tagtaatggc aacagtttca tggtttgcta tgagtgctaa
     1801 tttagtatcc agagtagtag ttactgctcc ttggacatta ccaggaccag taggggcata
     1861 tttagccact ggtggagatt ggagagcttc agttcttaac ttattatgta tagctatatc
     1921 aataataatt tactatccat ttgtcaagat gtacgataag aggttacttc atgaagaaaa
     1981 agttgaagag caagaagctt attaattaga aactatattg gaaattaaaa actatattag
     2041 attagtataa atttcaaagt ataacagtta tgaaataagt aacgaagata ttttgttgat
     2101 tgtttataaa aaaagaaaag gtaaactttt ctttttttat aaatatatat gaaacttttt
     2161 ttcaccaaat ttttaaaaaa gtttcgtgta attcgaaata aatttaaaca catataggat
     2221 ataaggagat gacatagatg ggaagattag gaatatcaat ttatccagaa aaaacaacat
     2281 ctaaggaaat atatgattat atagataaag ctgctgaaaa tggatttaca cgtatatttt
     2341 catgtttatt atcagttaaa gatactaaag aaaatataat taaaaaattt aaaagtataa
     2401 atgaatacgc taagtttaaa ggttttgagg taatacttga tgtaaatccc agagtatttg
     2461 gtgaattaga aataagctac aaagatttga ctttttttaa aaagacgaag gcagacggaa
     2521 taaggcttga tatgggattt acaggttctg aagaggcctt aatgactttt aatcctcaaa
     2581 atcttaaaat agaaatcaat atgagtaata atactaatta tatagataca ataatggact
     2641 accaaccaaa taaagaaaat ttgacaggat gtcataactt ttatccacat agatatagtg
     2701 gattaaattt agaacacttt ataaaatgta cagaaagatt taataagtat ggattaagaa
     2761 cagcagtttt tgtaacaagt caaaatgaaa ccacatttgg accatggcct gtaactgatg
     2821 ggcttccaac aattgagatg cacagaaatc tacctataga tattcaagta aagcatataa
     2881 tagctatgaa taatataaat gatataataa tatcaaattg ttatccaaca tatgaggagc
     2941 taaaggtatt aggttctatg agaaaagata tggttacatt tgatgtagca atagaggaaa
     3001 atatacgaga agttgagaag aagatactct ttgaagaaat acattttaat agaggggaca
     3061 tatctgacag tctgataagg tctactcaat ctcgtgttaa atataaagga tacaaatttg
     3121 aactatttaa tactcctaaa ataataaaaa gaggagatat agtaattgaa agtagtgaat
     3181 atggtcatta tgcaggggag ttacaaatag ctttgactga tatgaaaaac agtggtaaat
     3241 cgaatgtagt aggatatata agaaaagaag aattatttat attagattat attaaaccat
     3301 ggcaaaagtt taattttaaa ccctcaacaa aataatttat tatgaaaata ttaatttatt
     3361 ataaaaatat aattatgaaa gttggaatta aaatgtgttt aaaaatttaa tattaagaaa
     3421 atgaggaaat gatatgttaa taaaaacttt agaaaattta gttacagaag gtagaaataa
     3481 gaatacattg caaatagata aagaagatac attaggcata atagaattaa taaataacga
     3541 agataaaact gtagcctatg ctgttgaaga acaaaaggag agtatagcaa aagctgttaa
     3601 tataatagta gatagaatga aacagggagg tagattattc tatataggag caggtaccag
     3661 tgggaggata ggaatactag atgccacaga atgtcctcca acttacggag tggattttga
     3721 gcttgttcaa gctataatag ctggtggaaa tcaagcaata tttaaagcaa tagaaggtgc
     3781 tgaagatgat aaagaacttg gaaagcaaga tataatagat agaggagtta catctaagga
     3841 tgttatatgt ggtatagctg catcaggaag gactccttat gttataggtg caatggagta
     3901 tgctaaggaa ttggggtgtg cagtactatc aataactatg aatccgaata gtgaaatgag
     3961 taaaaaagcg gatttaccaa taaacattat agtaggggca gaagtaataa tgggttcaac
     4021 tagaatgaaa agtggtacag ctcaaaaaat ggtatgtaat atgttaacaa cagcttctat
     4081 ggttaagatg ggaaaagtat actcaaactt gatggtagat gttaagacat caaatgaaaa
     4141 gttggttgaa agagcaaaaa gaataataat gatagctaca aatgtaaaat atgatgttgc
     4201 agaaaaattc ttagaagaag cagataatag cgttaaattg gctatattta tgataaaaag
     4261 tggtttagat aaggatagtg caaagagtat cctagataga caagaaggtt atataagtga
     4321 agctttaaaa tcaatagaaa aattgtaaaa taaataatag tactgataac ctttcaatat
     4381 taaaaaaaca gcattggaag gttattttta gatattaagt attgaaagaa ttattaaaga
     4441 tattgtagga ggatagtatg tattttttag gtgtagatgg tggaggaacg aaaacaacat
     4501 ttacattagt agatgaagaa ttaaatatag taggaaccat aaccaaagga acttgtcatt
     4561 ataatcaaat aggatttgat aatctaacga aattattaat aactgggttg gaagaagttt
     4621 gtaaagatgc taagataaat gtagaagaaa taacgtatgc atttgttgga cttgctggat
     4681 atggaaaaat aaaagaagta ttgtatgcat tagaagtagc aacaaaaaat gcatatagtc
     4741 atataaatta tactctaggt aatgatgtag aaatagcatt agcaggttct ttaaatggag
     4801 aaaaaggtat aaatataatt gcaggtactg gttcaatagc acaggcttta gataaagatg
     4861 ggaatcttca cagatgtgga ggatggggct atgtattagg agatgaaggt tcagcgtatt
     4921 atataggaat ggctacctta aaaatgttca caatgcagtc tgatggaagg tgttcaaaaa
     4981 caaaattata tgatttaata aaaagacatc ttaatataga gaatgattat gacataataa
     5041 aatatgtaaa tgatgaaata caaggagata gaatagaaat agcaaagttt gctacgatat
     5101 gtttaaaagc agttattgaa ggtgataata ctgctagtaa aatatttgat gatgcagctt
     5161 atgaattaag taggcttata ataggattag agcatcattt tgaacaaggt acaaagataa
     5221 aggtatctta ttcaggtgga gtgtttaagt caggagattt aatattagag ccacttaaaa
     5281 aatatttgaa taaaacgaga tttggtatag tgagaccagt gttgactcca gatttaggag
     5341 catgtttact tgctaagaag aaatattata gtaaataggt agaggtgata gagtatgagt
     5401 tgcattttaa aattaaaaca gatatatgaa gatttaacgg aagtagataa aaaaatagct
     5461 gattacatac ttaataatac agaggctata agtaagcttt ctgtatcaga attggcgtca
     5521 aactcaaaaa caagtacagc aagtatagtt aggttttcta gaaaaatggg atattcgggt
     5581 tttggggatt taaaaattga gatagctaaa gatttgatgg gcaaagaaaa tgaatacaca
     5641 tatgtagatg aaaactatga ttgtaatata gatagtgtta taaacaaaat tacaaacaaa
     5701 aacatagaga ctatcaacca aacaaggttg ttgaatgaaa aagatataat aaaagaagca
     5761 gttgaacaga taataaaggc taaaaatgta tatatatttg gagtaggtgg ttcggcttta
     5821 gttgcactag atttacagat gaaactttta aggataaata aacaggcatt tacctcttta
     5881 gatagtcata cacaacttat ggtatcttca aatgtagata aagaagatat agctatagca
     5941 atatcttact ctggagaatc aaaagaagta ataaagtcta tagaaaatgc aaaattaaaa
     6001 ggatgtaagg tcatttgtat aactaaatat agtgacaatc atcttagtaa gattagtgat
     6061 ttaaagctag tagtaccaaa tatagaaaaa aggcttagag aaggagctat ttcttcaaga
     6121 atagcaatgc ttacgttgat agatataata tatatttcta taattcaaga aaacttaaat
     6181 gaggcagaac agaaactaca agaaacaaaa aggatacttg attatttaaa tattaataat
     6241 gcttaaaact aggttatata tcaactaaac aaataagtta ggctataagc gtaagtcaat
     6301 aagaaataga taaatatcta aaatattgac ttacttaata ataatatata gcatttttat
     6361 aatacaaatg agttgtctta aaattgattt tttttaatca aattttaagt tcaactcctt
     6421 ttttatattt ttatagatat atcctatatt ttcccagaaa acgtattgaa tatacaaatt
     6481 ataagaagta taatgttgtt gggagagaat gtatgaaatg tatacaaagt tgattttaac
     6541 tgtattattg ataattctct ttactcttac cattattaat gattgaagac aatggtattt
     6601 attataggtc aaagattccg aatgtattaa tcatcatcat tttctcaaac taacagcaaa
     6661 caccctgatt tgcgagttgt attgtataca aattatctag aaaatatcta ttagattttc
     6721 catgaaattt aaatatttca cgaatgtatg aatggagaaa tatacatgtt gacaatccac
     6781 ctttttaaat cctaaattaa ttttattaat ggtagagaat aggaagtaac aatgttttat
     6841 aaaggagaat attaagtatg aagaaaatta ggaagttagc taccaagtta ttgattacaa
     6901 caatagtttt aatctcaggt atgagtatga cagtttatgg tatgacagcc aaagaggtaa
     6961 cagcaaaaac accaaaaagc tatgtcactg gaaccaatag cgtatatggt ccaaagctgt
     7021 cacaagccca attaaactca gtagcacaag caacagcaga ttttatgaac aagaaaatca
     7081 caaagaatat gaccacagat gctaagattt tagttgccta caatcacatt aaaaataaca
     7141 caacatatgt tgactggaat gcagtagagg gagcaaatac tgcctataca ttggttacta
     7201 aaaaaggtgc atgctcagga atggctagga gcatgaaagc tttgtgtgat gctatgggaa
     7261 ttgaatctta ttatgtacac agcacatcaa atgaccacca gtggaatctt atccgttttg
     7321 gtgatgggcg tttgtatcat gtagatattg atgctaataa atcagcagga aaggatatca
     7381 tatacaagag tcttagccac ccattaccat ttgataagac agcatatcca aaagttggtg
     7441 caatagtaca gacagtaaaa ccagaacaga cacagaaacc agcaccacct gggaagcaca
     7501 ctatgttacc acatcaagaa ggggcaaagg ctgttggaaa ttttggaaaa aaaggagaag
     7561 atataaatga aaaacatgta atgtatgaag gtgttcgatg tagtgtagtt aattcatcag
     7621 taaatacaat tacatttaca ggtagtgaat caaatttaga cccatcaaat cttacgattg
     7681 ctgtaatagg atttatgaac acaccatatg ggatgtttgc attagcacca gagggtaaac
     7741 ctgttgaata tccttttaaa tatgataagc cattttcttt agattttagt ggtaaaaatc
     7801 gttctggtgc agaattagcg aagtactgta aaaaaaatga tgtgactatt aaaatagaaa
     7861 tcactgatga aaaagctaat attggacttg gtcaaataat gtatgttcga tatgaataat
     7921 gatttaatca tgacagtaga ataagttata aacattgcgc cctcttacta ggggcgcatt
     7981 taatattata tctgatattt tgtagtcagg tcaactgcta gatttctccc ctttttattt
     8041 ataaaaatta ttatctctaa gcatctgagc atgtttacga ttaactaaaa actgttttaa
     8101 ctcagtcaag cataggaata aaatagctct attttcaaac tctgttctgt caacaggtag
     8161 aggctggttt ttcatactaa gcattagtct ctttaattct tcaagaagaa gattgcctgt
     8221 ttcagcttca aattcagtat aacctatctt atgtataaaa gcagatagaa tatgagcttg
     8281 tggtggtgta gaatttagtt gagatacata actatacaaa ttttctaaga tatcccgttg
     8341 actttttata atatccatat gctctaagaa gaatctagta tcacttaata aagtattatt
     8401 aatattatcg taagtttcag aaatactgct gtcaataagt gaatttaatg tatataaatc
     8461 tctactatat ccattttctt ttttaggatt tacaataata tctgcaatgt ctataagtac
     8521 aatactcact tcttcctgta actttttttg aacttcatgg attttatgta tatttgttgg
     8581 aatatacata tttattaata cacctattcc agttcctact gcaaatattc cagcttcatt
     8641 taaaatccat ttgatagaag tttctccttg aaggtagtaa tgggatgcta tgactacaca
     8701 catagcaata acatcttgaa tatttaacaa aaaacaaagt ggtataatta taaatataaa
     8761 tattgaaaat gaagtcaaat tatatcctaa aatattaaaa cataaatatg aaaaaatagt
     8821 acataaaaga aaaccataaa tctttccaat ggagccttta agagtttctc ttttagtgtc
     8881 ctttactgta agtagtacta taactccagc aaccattgaa tattctaatt ttaatagcca
     8941 agcaataaat atagcaagag aacatccaag agataatttt attactttaa gtgaattaat
     9001 attattcaaa tgcaaacctc ctgtaaatca atataataaa attctaacat aaaactaata
     9061 ttgcttgaaa ggtaagtaac tatacttaat tattttattg ccctatttgt ttagatacaa
     9121 tcaatataat tttattttat gaaaattttt tgtaaaattt taaatttaga gactaacatg
     9181 agaccacaaa gaggaagttt attataggaa gaaaacttaa aattgatttt aagcagcaaa
     9241 tgaaaataat caaatatgta tatactatta ttaaaaaata aaattatata gttaatatga
     9301 ttttataatt ggtttagtat agagattttt aacttttatg ttatactatt tataaaagta
     9361 ggtataaaga ttaaaataca taatcaaata tgtataagag gttttaaata gtattgttaa
     9421 tgaacttgat tattgtttat aattacaaat tggaggagat aaacatgaaa attactgaag
     9481 gatatatgcc ttttaaaggt tttaaaacgt attaccgtat agtaggagaa aatacggaag
     9541 ggaagaagcc attagtatta cttcatggag gtccaggttc tacacacaac tattttgagg
     9601 tattggataa gatagctgaa agtggtagac aagtaataat gtatgaccaa attggttgtg
     9661 gaaattcatt tgtagaaggg catcctgagc tatttaatgc agatacttgg atagaggagc
     9721 ttattgaact aagaaagcat ctaggacttg atgaaattca tctgttaggt caatcttggg
     9781 gaggtatgca ggctatttgg tatgctattg aatataaacc aaaaggtatt aagtcttaca
     9841 tcctttcatc tactctttct tctgcaaaac tttgggagaa agaacaaaaa agaagaatat
     9901 catatatgag tgaggctgac caaaaagcat tacttgatgc agtaaatact ggagattact
     9961 ctagcaaaga atataatgat gcattagaaa gatttatgga aatgtattgt gcaggtgaag
    10021 tgacagaaga ttctccagag tgcctgagaa gacctaaaaa atcaggttct gaagcatata
    10081 ttgtaggatg gggacaaaat gaattttctc ctacaggaac tctttctgga tatgaattta
    10141 cagatagatt acatgaaata aaagagcctt gtctagtaac tagtggagca atagatttat
    10201 gttcaccata tattgcaaag actatgtatg atagaatatc taatagtaag tgggaattat
    10261 ttgagtattc aagacatatg ccattcgttg aagaaaatga gaaatatata aaagtcttaa
    10321 ctgagtggtt gaatgcgaac gattaaaaac tatataaatt tatataaaaa aatgagtgtc
    10381 tcaaaatgaa ctttttagtt cattaggcac tctttttgca tgaaatcaaa tatattttca
    10441 ttatttgaac aataaaatga ggcttatatc tattttggga aagtaatttt ttattactag
    10501 cgttagattc taatctaatt gtatacttat tgaaataata gaaatatatt ggtgtttata
    10561 acttaatttt attgtttttt agaaattttt ttatatattg gtataattat tctgtttaaa
    10621 tttgattaaa aagtatatta attataataa gaaacaatta tataaatagg aatagattat
    10681 tataattaat atagatataa aaataattat agaaggaaag gcacattatg gaaaaattga
    10741 gagttattga aatgataaaa aataatccca atattatagc tacaatagat aatccaactg
    10801 atgaaatgaa attgttagct attaaagaaa atgggcttgt actagaatat ataaataatc
    10861 caaccagaga gatgcaagaa ctagcaatag ataataatat tagagcaatt aaatttatag
    10921 ataatccaac agaagatatg atgataaaag cagtaaatga gggatggagt atactggatt
    10981 atatcaaaaa cccaactgat aaagttattg aaatggctat aaatcaagca ggatgggcaa
    11041 ttaaatatgc taaaaatcca agtgaagaat tacaactgtt agcagtaaga aaaaattatg
    11101 attcgataaa gtttataaaa caaccttatg aaagtgtaca agaggaggca gtgagaataa
    11161 gttatgatgc cttgagatat atagattcac ctagttataa tgcagaactt atagctataa
    11221 aaaataatga agcagctatt agctttataa ctgatttaga taaaaataaa atgttggaat
    11281 ttttaaaagt gaatatttta gtgattaagt acattctgaa aaaagtatct aaagatgaat
    11341 tagagagtgt attaaaagaa gtattatcca aagaagatgt tgaggaaaag tatattagag
    11401 attttttgaa ttgtagtgtt atagatagaa atagtgaaag tataccaata gataaaatta
    11461 tgtttattta taaatatgga agtaagacag caaaaaaaat agctgtagat gagaaattaa
    11521 agattatgta ggagagagaa aaatgaattt tatagacaca ttaaaaagcg ttgacttaat
    11581 attatccact ggagaagtac ctttaatagt aggtgaaagt ggaataggaa aaacagcttt
    11641 agcaaataag cttgccaaag aaaatgattg gagtttaatt gttatagatg gaaacttact
    11701 taaagagggt gaaataggtg gtcttccaac tatagaatct tatgtaggag ttaattctaa
    11761 tggatataag actgaaaaga aaacaacagt gtatgctgtt cataacaaat taagagaaat
    11821 tgatgaagag atatctaaaa gtaagacagt tcttttattt atagatgaga taaatcgttg
    11881 tgagcataca gtacagcaag aacttatgaa tttaatttta aatagagaaa ttaatggata
    11941 taaactgcat gatgatgtaa agattttagc agcaatgaat ccatcaagta aatatggttc
    12001 agattttgat tatcaagtgg tagatatgga tgcagcccaa gaaaataggt ttgtttggtt
    12061 aaatatggag agtgaccata cacaatggat aaaatgggca atagatgagg gtattgaaag
    12121 aaaagttatt gagtttattt ccacttttcc agaatatctg cataaaataa atgaagatga
    12181 tgtaagagct actcctagaa gttatgagag agtatctaaa atttataaag tttataaaga
    12241 aaaaaataat tctattccta gagcagtatt tttaaatgtt gtaaagggta atgtaggtaa
    12301 agttattgca gaagaattta taagttttat tgaatcaaac tctgaaccat taatatccta
    12361 tgaagatgtt tttttaggag aatctattga tgagtctatt gtagaaagag taaaaaatga
    12421 gaatcataca agactttatc tatcagctat gaacatttta aaagacttag aattaaatat
    12481 taagaatgat aaatatgaat caaaccacta tattaataga tttattgaat ttttaaaaat
    12541 gtatcctgta gatttaatga taggtattat gaaggatatt agaaacagtt atattgaagt
    12601 atataaaaaa gctatagaaa atgaagagtt tgtaaaatca tattttgaat cttatagttt
    12661 aataagggga taatgtatgg aaaattattt tgataaacaa gcaaaagagc tttataataa
    12721 agcaagtgaa attataaata ctcattctat gctaaaggca aatcgtgcta atgagaaatt
    12781 tgatatagat ataccacaag attttaagta tgagtttttt agtcttgtag ataaagtgaa
    12841 tttaagtctt atggaagaag aagataattt ttatggatat tttttgtttc agatgtcaag
    12901 agaaataaga tttgatataa gtagtcctac aggtgtgaat tttaagggag ctaaatatgt
    12961 tatatatttt aatccaataa tctttttaac gcttgacata aagcaaatgg agactacaat
    13021 taaacatgaa attcatcata tattatctat gcatttgatg agagcaaagg agttaaaagg
    13081 aaagtatagt acattagcaa ttaatatggc aatggatata gtagtaaata aatttctaaa
    13141 caatttacca ccatatgcaa ctaccttaga gtgggtaaat ttaaagtatt ccttaaaact
    13201 tgaaccatat gaaccttttg aatattatgt agaaaagatt cagactgaat tagatttgat
    13261 ggaagtggat gaagatggtg aggaagatga tagtgataaa tatggagata tagaaattga
    13321 ttatagcccc gaaagaactc atgatatatg ggatgaatcc aatgagatag atgaaaatac
    13381 ccttagagaa tttactgaaa aatttattaa cagttctcaa aaaggtaaag ttcctgctta
    13441 tttagaaagt atgatatcat cacttaaaaa tagtaagggt gaattgccat ggaatttata
    13501 tcttaataga ataatgggaa cagttgaaaa taataaaaag aagactataa caagaagaaa
    13561 tagaagacaa ccaaatcgtt tagatttgag gggagaactt agaagtcata agtcagaaat
    13621 agcagttgca cttgatgtaa gtggaagtat tagtgatgaa gagtttaaac aagctattaa
    13681 agaagttctt agtatagtga aaaactataa tcatgaaatt acaattatag aatgtgataa
    13741 tgaaattaga cgtgtatata aagtgagaac tgaaaaagat ataaaagata gaattaggat
    13801 aggtggaggc actagatttt ctccagtctt tgagtatgct aataataaaa agattaattt
    13861 gttagtctac tttactgatg gtaaaggtga aaataaacta caagtaatac ctagagggta
    13921 taaaatttta tgggttattt ctggaagagg agataaaatt tcattaaaag aaccttatgg
    13981 agcagttaaa aaacttacta aggttaaaac aaatgatgaa atattagaaa taaatgatgt
    14041 aagaaatgat gggtactcaa tgaatagcca agcaccaatt ttataggtat tcacaggaga
    14101 taagctttat taatttattt tgatatgctt gttataaaaa tctaaaaaat acattaatca
    14161 atggttaatg tattttttaa ttttaataat ataagtttta cttacattta agtcttaaaa
    14221 tgtgttaaaa tataatatat agcagatagt tttggcaagg tggatttagt tgtcaaaact
    14281 atttttttta ttaaggatgg tgatgttttt gtttaagatt gatgattata taatgtatgg
    14341 tatgacagga gtctgcaaag tcatggatat aacaaatgaa cgatttacaa atggaataaa
    14401 aaaagagtat tatgtattga gtcctattta ttctaataat acagtaataa aaattcctgt
    14461 agataatgaa aaagtaccaa tgagaaagtt actttctaaa attaatgtgc tttcattaat
    14521 aaatgatatt cctaatatgg atacatcatg gatagataat gaaaaattga gaagtgagca
    14581 atttaaaaaa atactcagag gtggtaaatg tgaagagtta ataaaattag taagaagtat
    14641 agataataac agagagtatg taaaatctat tggtaaaaaa acacatcaag cagatgataa
    14701 tataatgaaa gaagctgaaa ggttattaaa tgaagaattt gcaactattt tagatatttc
    14761 tcctaatgaa gttagttcat atatttcaag tcatatacct caataaagtg ttttagtatc
    14821 ctttatttaa tctattttaa gtattattta tgtatgctgg ttttggtaaa ataaaatata
    14881 taattaaatt atataaaaaa taataatgat tgtattgaag aattacagaa tagtgcggag
    14941 agcctactcc tttaggtgtg agatggatat cacttaaaga tttaaattgt tattgtagat
    15001 gtttttaata taaaacaaga ttaataagtg tattgtaagc tttaagtgaa attgatacta
    15061 taatgagtta cttagatttt aaaatatata atattataaa aaatcctttt aggttttgaa
    15121 tgaataagat ggatactatg ttgaatagaa tccatcttat ttaagtttta tttatgttgt
    15181 ttatttgtaa gcattatttt tgtacatgaa aacacagtgt aaaaatatgc ttagaattat
    15241 gaagttttat taaaaagata ttcgacaaaa taagactaaa aatattctta aacttataac
    15301 taagataaat attgtatatt tatagtgtag cctgaaaaat tgtgaatgta cgaaatttat
    15361 ttagtatttt attgaaacaa agtagaagaa gtactatagt ttatctttga atagattgtt
    15421 attttttagt gaaaaagtta ttaggttaat acataaatat aatagcttat aaatagttta
    15481 agcatgttaa aaaagtagtt attagagata tatagttaca ttgcttata
//
LOCUS       DAFKMX010000101        15273 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFKMX010000101 DAFKMX010000000
VERSION     DAFKMX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.101"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(114..974)
                     /locus_tag="LZB08_002370"
     CDS             complement(114..974)
                     /locus_tag="LZB08_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBM7315867.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1123..2013
                     /locus_tag="LZB08_002371"
     CDS             1123..2013
                     /locus_tag="LZB08_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7315868.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2231..3985
                     /locus_tag="LZB08_002372"
     CDS             2231..3985
                     /locus_tag="LZB08_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBM7315869.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            4370..5356
                     /locus_tag="LZB08_002373"
     CDS             4370..5356
                     /locus_tag="LZB08_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBM7315870.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     IRPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            5777..6628
                     /locus_tag="LZB08_002374"
     CDS             5777..6628
                     /locus_tag="LZB08_002374"
                     /inference="COORDINATES: protein motif:HMM:NF012703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HBM7315871.1"
                     /translation="MKTIRLLYPDYVSGGLEQYFFGANLMSHILPENENQPIVKVEIT
                     PPNRKEKLVTDGIYGKNEVIAGIKNATEKIEKENPDKIITIGGNCLVSQAPFDYLHGK
                     YNDLGIIWIDAHPDVSTVNDDYPNAHAMVLGSLMGYGDNALSSLMKNKKFKADEILYV
                     GLQEIHDYQKKFLDETCVNYKIQTDEFLSDKEILSFIERFENIVVHFDIDVLDENLFH
                     STYFANPDLVGDGSGGGKMTLDKLGNILHCITENSNVVGFTIAEYLPFDEYKLHKLFS
                     KIRMFTE"
     gene            7192..7812
                     /locus_tag="LZB08_002375"
                     /pseudo
     CDS             7192..7812
                     /locus_tag="LZB08_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8225..9706
                     /locus_tag="LZB08_002376"
     CDS             8225..9706
                     /locus_tag="LZB08_002376"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBM7315872.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKIKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDKSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            9732..10703
                     /locus_tag="LZB08_002377"
     CDS             9732..10703
                     /locus_tag="LZB08_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBM7315873.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEANVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(10959..11561)
                     /locus_tag="LZB08_002378"
     CDS             complement(10959..11561)
                     /locus_tag="LZB08_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7315874.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDSSIEVDNC"
     gene            11842..13053
                     /locus_tag="LZB08_002379"
     CDS             11842..13053
                     /locus_tag="LZB08_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBM7315875.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEVELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYELPK
                     QYYKRKDGYIE"
     gene            13121..13318
                     /locus_tag="LZB08_002380"
     CDS             13121..13318
                     /locus_tag="LZB08_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBM7315876.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            13352..14323
                     /locus_tag="LZB08_002381"
     CDS             13352..14323
                     /locus_tag="LZB08_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7315877.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            14510..14887
                     /locus_tag="LZB08_002382"
     CDS             14510..14887
                     /locus_tag="LZB08_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7315878.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         6112 a         1749 c         2515 g         4897 t
ORIGIN      
        1 catttaattt ttatcttaaa aacaacaatt ataatatata aatagcaagt ataatataac
       61 atataaaaag ttgaatttgt atatgttaat tatacttgct atttatttgt tagttattct
      121 atttttttaa atccattacc taaagcttca tgtatatctg taattataat aaaagcatta
      181 gggtcgacat tttttactaa ttttttcaat tcgacaactt ctttcttttc aactactact
      241 aaaagtacat ttttattttc tccagtatat gcaccttctc cttttagaat agttgcacct
      301 ctatctaaat cttccattat ttttttactt atatcttttg aaaggtcaga tataatagtg
      361 aatgatttgg aataattaaa tccttcaata agcaagtcac taacttttac taaaatgtat
      421 aaagccacag ttgaataaag tgcaatttca acctgtctat ttactatacc tgataaaaca
      481 actacaacgc agtctacaat acccattaaa acaggtatac taaatgtagg aaaatattta
      541 tttagtagga gtgccattaa atcagttcca cctgtagagc catttattct aaaaataagt
      601 ccttgaccaa ctcccattaa aatagaacca gatataattg caagatacat atcatttgtt
      661 atatctaatg tagccatatt tgcagtaagc ttaagtgcta tggtaagaaa aattattcct
      721 aaaaccgttt taaaacaatc tttttttgaa agtattttgt aagcaaatat gaataatgga
      781 acatttaaaa gtaaattgac aatccaaagt tctacaccga ataatgaatt taaaacaact
      841 ccaagtcctg ttataccacc agcagcgatt gcatgtggat taaaaaataa gttaagagaa
      901 atggccatta atatacagcc tacaattaat attaaaattt ctgaaaaagt tattctatct
      961 tttttcaata acatcactcc ttatatatag attataatat atattatata gtttttccaa
     1021 tttttaataa aagaaataaa aatatattat aaaaacaatt tgattggaat tttcagaaaa
     1081 ttaatgctat aattaaaagt aaatttaaag ggaggttata aaatggctaa attaactcta
     1141 gaacatataa aaggaaatac atattatatt ccactcccta caatagtagg tgtatacgta
     1201 gatggtaagg atgcaatttt aattgacagt ggaaataata aggatacagc tagacaagtt
     1261 ttaagattat tagaagaaca taatttaaat cctaaattaa taataaacac tcattcaaat
     1321 gcagaccata taggaggaaa tgcatatttg aagaatcaaa caaaatgcaa gattgctacg
     1381 actaaaattg aaggatattt tacagaaaat ccaatattag aatcagcatt tttatatggt
     1441 ggctatccaa gtaaagcact gaagaataaa tttttattag caaaggaaag tgaagttgat
     1501 tatattatac cttcaaatgg caaaattgta gatacagaac ttgaagcgat ttctcttcca
     1561 ggtcattatt ttgaaatgat tggtgttaga actccagata atgttttgtt tgttgcagat
     1621 agtcttacac cagagaatat aattactaaa tatcattttt tcttcctact ggatatagat
     1681 tcacagttta agacactaga aaaattgaga atgttagagg ctgatttctt tgttccaagt
     1741 cactctgtca aaactacaga tataaaaagt ttgattaata ttaataagaa gaagatggaa
     1801 gaaataatag ataatattaa aaaggtttgt tatgagccag ttatgattga tgaagttatt
     1861 gaaaaaatgt gtgatttgta caatgtcaag cttgatgcta atcaatatgt gttggttgga
     1921 agtacaatac gttcttatat aacttattta tatgagaata atatggttga atatatattt
     1981 gatggtggta agatgatgat aaaagctgtg taaatagtaa taattttcaa attatatgat
     2041 gaaattgttt tagtatgact gttatttgaa aagtaatata aaaaaattta tataaataag
     2101 ctaagatgat tgatataaag ttactgataa ttttaaggta atatatacgt tactataata
     2161 ttatgttatt atttgtaaat aaggggataa tttataagca aattaaagac cttaatttac
     2221 aggaggaata gtgtatatta tgaataaaaa attcaaaaaa ttcagtaaaa aactaagtat
     2281 attaatttta gttttgacta tgtttctttt taataattta actgcaaatg cacagattaa
     2341 agaagattca ataataggaa ttgatagata tgaaacagca ggacttatag cggatagaca
     2401 aacttatgat actgtaatat tagttaatgg agataaaaat ttatctgatg ggctttcatc
     2461 aagtggatta gctggagcta ttaacgcacc aatacttttg actaaaaaaa gtgaaatacc
     2521 aaaagctact ttaaatagac tagataataa gagtttaaat gttgtaaaaa aagtatatat
     2581 aattggtggt tataatacta ttgaaaattc agtagaaaaa gatataaaaa gaaaaggtat
     2641 tcaagtggaa agaataaatg gtaacaatag aattgaaact agttataatg tagctaaaaa
     2701 aataaatgaa gtaagtagtg taaaagaggt aatgcttaca aatggatttg taggagaggc
     2761 tgatgctatg agtatagcac ctgtagttgc aaaaaataaa ggagctataa tacttacaga
     2821 tgggaaatca gttccattta agactgaaag tttaaatgtt tacgctatag gtggcaaatc
     2881 tgcgataaat gaggatttag taaatgaaac taaagcaact agaatcggag gaaatgatag
     2941 atttgagacc aataaaaagg taatggaaaa gttttataat ggagcgacag atttttatat
     3001 aacaaatgga tatcaattag tagatgcact aacattatct cctttagcca aagaaaaacc
     3061 aatcgtttta gttgctgatg gaagtaataa aggtatttta aaaggagcta aaagtataac
     3121 aaaagttgga ggtattgatg ccaatatata taaacagtgt ttagatatga ttaaatataa
     3181 taatatggat atgactccaa atattttaaa acacttaact tcagttgaag gaaatgaagt
     3241 ttctgaaata cttatagaat cagataattt aggtgtggtt gttgaaaaga cgaataccaa
     3301 taaatttgag tttgattacg taagtgtaac taatgaaaaa aattgtactt tttctgtata
     3361 taaagaaaat tcttctaata atataaaaaa tggaaagcta gttgtaagtg ctaagaaaaa
     3421 aatagataag aaagatagtg atttgcaaga tatgaatggc gacaatatga taaatgcaaa
     3481 taaagatagg atggtaaatg tgataaaaat tggagttccc gataaaatgt attctaattt
     3541 taaagtagat gttgaaagtg gttcagtaga actttataat attaaaggag gagctactgt
     3601 tgatgtcaat gacgggattg ctaacattgt tgataacaat gtaacaaatc ctttcaatat
     3661 aaatactaat agtgggattt caggtgtaac agctgaaact atatcatctg aaataaaatt
     3721 tagagcaaac aatggaattg taaatgtaac agctacgaat ataaatggca atataagttt
     3781 atttggaagc gataataaag gtacctttga tggaatattt aaattaaatc tgaaaaaaga
     3841 accatcaaat ttacacttga agttaattgg aaatgggata aataaattgc cagaaggttg
     3901 gtctaaaaat tatattttag gaaatggaca accaattatt gaagttaaaa ataatggcat
     3961 aaataatata tcaattggag aataaaaaca tatgaaaata aagtttatat agttttaaaa
     4021 attatagaat tttaaaatat ttatatatat tctttaaata aaaaattatt ttttaagtga
     4081 taattaagta tttatcgata aggctatggt tgtaaaaatc atagccttat ttttatatta
     4141 taaataaatt aaagtattta tcaattcata gtagataaaa ttgaaattaa atataacaac
     4201 aaaaaatgaa aattacaatt aatttaatgt taaaggctca attctttgga tgtggatatg
     4261 gatagtacaa gtagtatata aaaagtattg actggacaaa taactatatt ttattttaaa
     4321 tatataatat aatgtaataa aatatatcaa atgtagaagg tgataaccta tgaaaaaaat
     4381 aacaataaat gatattgcta acttggcagg agtatctaaa agtactgtat caagatatct
     4441 aaataataaa gatatcagtg attctactaa agagaaaata aagacaataa tagatgaata
     4501 tggatatgag ccgaatgcat ttgctcaaag tttgagagct aaaaaaacat actttattgg
     4561 tattataaca ccgtgtttag attcttttgt aaaatctaaa attatgatgg caatagatga
     4621 agaactcaaa gaattaaaat atacatcact aattattaat actaatagaa aaataaggtc
     4681 agaaatagat agtatatcaa agttagctag tctaaaagtt gatggaataa tactaattgg
     4741 taccgaaata actaaagaac ataagaatga aattgaaaag ttagatatac cgatagttgt
     4801 agttggacaa aaagttgatg gtatcaatag catagtgaat gatgattatg gagcaggata
     4861 taaaatgggt caatatattg ctaataaagg ttacaaaaac attgtatatt taggtgttga
     4921 tgaaagtgat atatcagttg gtttaaatag aaaaaatgga gtacttaatg ggttaaaaga
     4981 taaagggtat gatgcaaaag tattttatac tgactttgac caagaaacgt ctatacaaag
     5041 aagtggagaa atgctagaaa gtgaaaatcc agatataata atatgtgcaa ctgataatat
     5101 agcaatagca acaatgaaag aaatcaataa gagaggtaaa agtataccac aagatatatc
     5161 tgtagctgga tttgggggat atgatgtatt gtctattata agacctaaac ttacaacaat
     5221 aaagtttgaa aataaaaatg caggtaaagt agcagcaaat acgatagtaa atttaataca
     5281 agaaagaaaa gaaccactat taaaggagat taaatttgaa ttaatagaag gtgaaagtac
     5341 aataaataaa aattaagata cacattacat atattagtaa tgtgtatttt ttacgtgaaa
     5401 aacagataga aaatcgaaaa tatccacata aatacagtat ctatcaacaa aacaagcaaa
     5461 agaaaatacg gaaaattaca acaaatgaaa gagccttatg taacgactta taaagagaaa
     5521 tacaaagata taagaaaacg gcacttgaac agagtgtctt tttctatgca gtggaacagc
     5581 atactatttc atttctgctt gattacctta aaacttatta gtctatggtt tgtcaatggc
     5641 ttattgtgta agcaatgttg aatagcaccc tttacaaatc atggactggt aagtaacttt
     5701 tcaaaagcag aaaagaaatg acgataaaaa tatatcatgc tataattgaa gaaaattata
     5761 attttaggag gataccatga aaacaatacg attattatat cctgattatg tgagtggtgg
     5821 tttagaacaa tatttttttg gcgcaaattt aatgtctcat attttgccag aaaatgagaa
     5881 tcaacccatt gttaaagtgg aaatcacacc accaaacaga aaagaaaaat tagtgacaga
     5941 cggtatttat ggaaaaaatg aagttatagc aggaataaaa aatgccacag aaaagattga
     6001 aaaagaaaac ccagataaaa taatcacgat tggtggaaat tgtttggttt cacaagctcc
     6061 atttgactat ttgcatggaa aatacaatga tttaggaatt atttggatag atgcacaccc
     6121 tgatgtttca acagtcaatg acgactatcc aaacgcacat gctatggtac ttggttcttt
     6181 aatgggatat ggggacaatg cgttgtcaag tcttatgaaa aacaagaaat ttaaggcaga
     6241 cgaaatatta tatgttggac ttcaagaaat acatgattat caaaaaaaat ttttagatga
     6301 aacatgtgtc aattacaaga tacaaacaga tgaattttta tctgataaag agattttgtc
     6361 gtttatagaa agatttgaaa atatagtcgt tcattttgat attgatgtac ttgatgaaaa
     6421 tcttttccat tccacctatt ttgcaaaccc tgatttggtc ggcgatggtt caggcggggg
     6481 aaaaatgaca ttggataaat taggaaatat tcttcattgc attacagaaa attcaaatgt
     6541 tgtaggattt acaatagcag aatatctgcc ttttgatgaa tacaaactac ataagttatt
     6601 ttctaaaatt cgtatgttta ccgaatagct tatttgatta acgtggaata tttcaaaccg
     6661 acggagtggt ccgcccactg tggcggtcgt aagacatatc gccactggct aggggtggtt
     6721 cacttcggcg gcttggcaag cgaagcgagc cttataagcc ccgttatcta acaggggggg
     6781 gactattaca agaaacaaga gacagaacaa ttacaaaaat cccgatatgc aacgatttat
     6841 aaggttataa gcacttactg tacttcgcac cttgtcaatt ggtggaaaac agtatatcga
     6901 cagcaagaaa atacatgata caaggtgtat gctcctatgg gcgtatgcct tttttgattg
     6961 gaggaataaa acatgaatga cattgaattt attgaagcat tgaaagaaaa gcggaacgcc
     7021 tgcggttatt cccaaacaag actgccaaag gaactgcaca tcagcaggca aaacttaaat
     7081 tattcatgaa gattacggac tgtttggcta tgaagaacaa tatatctatg gagagattat
     7141 cgttacagct tctaaagacg tttctatggg cgttttacgg aattgagggg tatgggctgt
     7201 cggcacttgg aacacgtctt acaggcaagg aaaacaggct tgcgtatctc ggagctttgc
     7261 ggactgacaa agaatgattt ggatttcaag aaccatgtaa tccgtatcaa tcatcagcta
     7321 cttaaagata aggacggttt ctacatagca tagccaaaga caaagagcag tttccgcaac
     7381 gtgcctatga gcgaagaaac agagaatgcc tttcgccgag tgctgaaaag gaagcagaag
     7441 ccaacgctca aagatataga tggctaccgc aatttcctat tcatcagtcc caacggaaac
     7501 cctatgcaag aaagcaatta taaatcggtg ctgaacggtg ttgtaaagaa gtacaacaag
     7561 aaccatgaga aaacaccgtt gctgaagata acaccccaca gcctaagaca tacgttctgt
     7621 acacagcttg tacagaagaa catgaacccg aaaaatttac aatatacaat ggggcatgca
     7681 agcataacga taacgctgga cttatacgcc tatgcgtcag aggcaggagc aaacagagaa
     7741 ttgcgaaagc atgatagcat gatttacaac gatttgtaca actattgcga gcagaaatat
     7801 aagggattat gaccagttcc acagtatacc tgcaacatga aaaaaacgct agacccttat
     7861 aaacagggat atattgacgg ttcgtgatag ttataaaaaa atactaaaaa atctatgctg
     7921 ttttttacta aaaaactata attctaaaaa ttaggaaaac ttttttgaaa gaaatattgc
     7981 atctgaaata aatttatgtt aatctttaat taaaccggtt tcgaaaccgg tttaattaaa
     8041 ccgatagaat agcaaaatga gtgcaaaaat aactgattgt tacaaagtat caaagtaatt
     8101 attagagtac ataggaaata aataaaatat aaaaggtgta gcacattgtg taataagact
     8161 aagaattgtt ttagatagca ataataaagc aaatataaaa ttataaaaga aaaggaataa
     8221 aaatatgtac aaagagccta aatatagaac catattagaa tcgacaaaag aagaattaaa
     8281 taaattaaga aatatatctt taaatgataa atataaacca ctatttcata tacatccaca
     8341 acatggacta cttaatgacc caaatggatt agcatattat aatggtaagt atcatgtatt
     8401 ttatcaatgg tatccatatg atgcaactca tggaatgaag cattgggctt atgtaagttc
     8461 tgatgatttt gtaaactgga atagagaaga tgtagcatta ataccaatcg aaagttatga
     8521 atctcatgga gcatattcag gtaatgcgat agaggtagat ggaaaattgc atatgtatta
     8581 tacagggaat ataaaatata gtgcagaaga tagatatgca tatcaaaact tagctataat
     8641 gaacaaggat ggtaaaataa ctaaatatga aaataatcca atagtaagtg aaataccaaa
     8701 aggatataca ggacatgtaa gagacccaaa agtatttaaa ataaaggata aatactttat
     8761 gttacttgga gcacaaacaa gtgataaaaa aggagttata atagtatatg aatctaaaaa
     8821 ctctatagat tggaatttta aaggagaatt aaatgtaaaa aatatagatg aagattttgg
     8881 ttacatgtgg gaatgtccag attatattaa tatagatgaa aaagatatat taatattttc
     8941 accacaagga gtagaaccta aaggatttga ttatcaaaat atatataatg tagtatatgc
     9001 tattggtaat atggatttag ataatttaac atttgaaata gatacaatga aagaactgga
     9061 aaaaggattt gatttctatg ctccacaaac atttataaag gactcacaaa taatactatt
     9121 tgcatgggct ggaatgggag aagttttata ccctacagat aaaaacaaat gggcacattg
     9181 tctaactgtt ccaagaaaat taaatataaa aaataataag ctattgcaaa tgcctgtaga
     9241 tgaattaatt aaattaagat atgatgaaac aagtgggcaa aatactataa agaataatat
     9301 caatattata gaaaataatg aaaatgtata tgagcttaat attaatataa aaaatattga
     9361 cagcaataaa tttgggctgg aattattttc atcacaagat gaaggtgtaa aattagaatt
     9421 taacaaatta ggaaacattg taactttaga caaaagcaac tttaaaaaag ttttcggtgt
     9481 tgaatatgga acaaatagaa aagaatatat aaatattgat gaaaatacta atatcaaagt
     9541 actagcggat agaagtattt tagaaatttt tataaatgat ggagaagtag tatttactag
     9601 tagaatattt gctaaagaaa attctaatca aataagggtt tatagtgata aaatagtaga
     9661 gtatgagtat actaaattta aattaaagca aggtattgaa ttataaaaac acattataag
     9721 gggtagaaaa gatgaaaaaa gtaattagca taggagaagc tttaatagac tttataccga
     9781 atcaaaatgg tggtaaatta aaaaatgtat cagaatttag aagagtagca ggaggagctc
     9841 cagcaaatgt tagtgctgtt gtagcaaagc ttggaggaaa atcaagtttt atatctaagt
     9901 taggaaaaga tgcatttggt gattatataa tagatgtatt aaatgaagca aatgtaaata
     9961 ctgattatgt attgagaaca agtaaagcaa atacaggact tgcttttgta tcattaaaag
    10021 aagatggtaa tcgtgatttc tcattttata gaaatccaag tgcagatatg cttttagaag
    10081 ctgatgaagt taaaaaagag tggtttaata attgtcatat cctacatttt tgttcagtag
    10141 atttaataga tagtccaatg aaattagcac ataaaaaagc aatagaatac gcacttgaaa
    10201 gtaatagtat aataagcttt gaccctaata taagattacc tttatgggat agtgagcaat
    10261 cttgtaaaaa ggcaatatca gaatttttac cttttgctca tattgttaag atatctgatg
    10321 aagaattaga atttataaca ggagaaaatg atatagaaaa atcattacat aaactctttg
    10381 tggggaatgt tgaactagta ttatatacaa aaggaaaaga tggagtagat gcatacacta
    10441 aaaaagtaaa aggtatgtgt aaaggggtta aagtaaatgc aatagataca accggagcag
    10501 gagattctta tataggttca tttttataca ccttattgta taaacaaata acattgaatg
    10561 atataaaaga aatgaaacaa gaaacgttaa atgagtattt agaattttct aattattatg
    10621 cagctcgtag tacaactatt aaaggtgcga taagttcata tgcaacaaag gaagaaataa
    10681 ctgaatttat aaaaaattta tagtaggaat gagtaatagg ttttgaagga agtaacatgt
    10741 aaagagtttt taaggatgtt tactatgaaa ataattaaca tataaaaagc aataaagagg
    10801 ctgtctcaaa atagagataa gcctctttat aattgttgaa atgataaaaa atattttaat
    10861 tattgttact ttttagaaga atatatatct tattttaaat aagcaaatta tgtaagattt
    10921 atttatatta tcaatacttt acttgaagta ttatgtcttt agcagttatc tacttcaatt
    10981 gaactatcta aaatataata aatttgtttt gcaaattgac gagaatgttc ttctttttct
    11041 ttattcataa aattaggttt acctaaatca cgttcaacaa gcattcctat atattttaac
    11101 tgtgaatgct tacagtagcg cttcatacca ttttcccaaa tatcagcacc cttttcaggt
    11161 ctatatccac atgttgtaat aagtgctact ggttttcctg cccatagagc ggggcctttc
    11221 tcattaccat agtatttatt cattccatac accaatctgt caagtagtgc tttcattgga
    11281 gaagtgcagt accaggaata aattggagta gcaaaaacaa ttaaatcaca cataagaatt
    11341 ttcttgaaaa ttttctgaac atcatcttta caataacatc caaaatcaaa taagttatcc
    11401 tgacattttt tacaagcaat acatggctct attttacaat catataacca tattacttca
    11461 atatttgaat tataattttc taattcctca ataaaaggtt ttaaaatagc agcagtatta
    11521 ccttttttct taggacttcc cataagaatg catacttcca tattgatacc tccacagtat
    11581 tttaaaatag tttttagtgc ttaatatagt tttaggatac tatgagaata cgttaacaac
    11641 aatacacttt gtatatttgt aataaaattg aaacaaattg ttttatcaat aaaagttgga
    11701 atgtatctat aattaaaaat attttaggta tgtttttaca tatatgtgtg tagttaacta
    11761 tggtaattta ttgtaaaatt atcttatagg attataattg cttaatttat gattatattt
    11821 aaaatcttgg aggtaaaaaa tatgtactca aatgaagact ataaatttat agaagaaaat
    11881 gcaccatgtt atatatatga tgagactaat ataattgaaa aatgtaagga attgaaaaat
    11941 aatattttag gttttgattt tttatattct ataaaaacaa atccatataa caaaattgta
    12001 aaaagcattg cagagcaagg ttttggagca gatgcttctt caactaatga agttatgatt
    12061 tcatcagaat gtggagtttc taaagatgat atttattatt catcaccagg caaaacagag
    12121 attgatatag aaactgtttt agataaatgc ataattattg cagatagtat tacagaagta
    12181 gaacttataa ataagatatc taaaaagtat aatcagaaag ttaaaatagg aataagaata
    12241 aatcctgatt ttactatgga tgatgatagt gctaaatcaa gcaaatttgg tatagatata
    12301 gaagatatga gcagtgtttt taagatgtta agtatgtgtg attatgtatc tgtagttggg
    12361 attcatatac atattaaatc tcaggtattg gattttgaaa agttaggaca ttattatgaa
    12421 aaatgttttt ttatagcttt aaatttaaat aaaagtaagt atattaatat tgaatttata
    12481 aattttggta gtggacttgg agtagcttat gataatctta gagaaaatcc acttaatctt
    12541 aaagagcttt caaaattaac tcaaaagatt gcttttacta attcaaacac actaaaatca
    12601 agacttctta ttgaatcagg acgtttcata gtttgtaaat caggtaatta ttatgttaaa
    12661 attgtagata ttaaagtatc aagagggaaa aaatatctta ttgtaaaaaa tggacttaat
    12721 ggatttatga gacctattat ttctaattta attgaaaata ttacttgtgg agtagaatta
    12781 gaaggtcaag agccattata tacatcaaat catgcctttt ctctttcagt attaaataat
    12841 ttaaaagaag aagagtatgt aaatgttgtt gggaatctct gtacatcatt agatgtaatt
    12901 gcaaataatg ttaaattgaa aaaagcaaac ataggagatt tgatttcaat tagtaatgct
    12961 ggaagttact cctattcttt atcaccacta ttattttcga gttatgaact tcctaaacaa
    13021 tattataaaa gaaaagatgg atatattgag taaacaatag cataaattaa taaacttagt
    13081 attattgaaa aaatttaaaa tgcagggggt gtaattctat atggaattgt ggaaaagaaa
    13141 tttatttgta tgttggatag gtatgttttt tagttctata gggatgagcc aaatagcacc
    13201 tatacttcca ttatatatta aacagttggg ggtaactgat gttagtttaa tacaacagta
    13261 ttcaggtata atttttggat gtacctttgt agtagcagcg ttcttttcgc caatttgagg
    13321 gaaagctgct gataaatatg gaagaaaatc aatgctttta cgtgcaagta ttgggatggg
    13381 aatagttata tttaccatgg cgtttgttca aaatgtctat cagttattag gattaagaat
    13441 acttcaagga gtttttacgg gttatgctac tgcatgtact actcttattg caacacagac
    13501 agataaaaat cattcaggat gggctttggg tactcttgca actgcatcaa ctactggctc
    13561 attaattggt ccaacagtag gaggatatat agaatcaatc ttaggtctta aaagtacatt
    13621 tattataaca ggaggattgt tgtttgtatc atttatcatc tctattttat ttgtagtaga
    13681 taattttaaa cctaaagaaa taaaagaatc aatttctata cctaaagaac aattaaatat
    13741 attgccaaat aaatttttaa ttgcatctat ttttgtaacc acattcatta ctcaactagc
    13801 tttatactct atagaaccaa ttgtaaccat atatatatca caacttacaa attttgcatc
    13861 aaatgtagct ttaatagctg gattgacatt ttctgcatca ggattagcaa atttattagc
    13921 agctcaaaaa cttggaaaaa tgtcggataa aataggtcct caaaaagtat tgttaatttc
    13981 acttttatgg gctgggatta tatttatacc acaagctttt gtaaaaactg catggcaact
    14041 gatgttgttg agatttttat tagggttgtc agttgcaggg cttaatcctt ctgttaacag
    14101 cttgcttaaa aaaattgcac cagaagaata tgtgggaaaa atatttggat ataatgcatc
    14161 tgcccagtat ataggctgtt catcaggagc atttttaggg ggacaaatat cagcacattt
    14221 aggtattaga acagtatttt tttcaacaag tttattgttg tttatgaatg cactttggat
    14281 gtataaattt actggattgt ttattaaaga taagactaaa taatcatact atttacattt
    14341 ctaattttat gatgtaaaat aaatattttt tatgtaattt atcatagaat tatataaaaa
    14401 ataaccctat ggtaaaaatt ggaaaatatt attatcgaca aaatctttga cttaaaaact
    14461 atattgcgat agtattaatt taataaaagt ttgagagggg cgtaaggtta tgacaatttc
    14521 aaaaatacca caagcagaat taaaagttat gaagtttatt tgggaaaaaa atgatacagt
    14581 aacatcaaaa gaaactattg aagctatgga acgaaaatat ggttggaaac aaacaactac
    14641 attgacactt ttatcaagac ttgttaaaaa acatttttta agtgcagaaa aaatagatag
    14701 atacactcat tatacaatag ttgttggaca taaggagtat ttaagtgttg agacaaaaga
    14761 ttttttaagc aatatacacg acaattcttt acagagttta atttcagcac tacatgatga
    14821 tgaagttgtt gataaagata tgttggattt atttgaaagt cattttaaaa atttagaaga
    14881 agagtaatta tatcaggata gaactctaag aatttaatta atctatggct gaaaaccata
    14941 aatggtatta tttaatcagc tactcactat tcataaagta atattaaata ggttctctaa
    15001 ttagtaaaat gcctaaaata attggaatag ggagtatcaa gttaaatgat accacttatt
    15061 taaataagaa atgattttta aaatttaaaa taattttata acttgtcaat attcattaat
    15121 ctaaagtatt atttctattt aaccatatca aaatatattt tactattttt aattattaca
    15181 atataaacct gtcttttgtt attataggta aactaaaagt aagaaatgat ttttatattt
    15241 ttaaacttta ttattggtaa cttatctgta aat
//
LOCUS       DAFKMX010000102        15119 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFKMX010000102 DAFKMX010000000
VERSION     DAFKMX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.102"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(302..1045)
                     /locus_tag="LZB08_002383"
     CDS             complement(302..1045)
                     /locus_tag="LZB08_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBM7315879.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DKLIMNYPKKLINHFHQKDKLLDVDYTNI"
     gene            1211..3778
                     /locus_tag="LZB08_002384"
     CDS             1211..3778
                     /locus_tag="LZB08_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315880.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIVKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4008..4412
                     /locus_tag="LZB08_002385"
     CDS             4008..4412
                     /locus_tag="LZB08_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7315881.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NRDGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4436..4933
                     /locus_tag="LZB08_002386"
     CDS             4436..4933
                     /locus_tag="LZB08_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315882.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            4936..5337
                     /locus_tag="LZB08_002387"
     CDS             4936..5337
                     /locus_tag="LZB08_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBM7315883.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5355..5843
                     /locus_tag="LZB08_002388"
     CDS             5355..5843
                     /locus_tag="LZB08_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315884.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            5870..6625
                     /locus_tag="LZB08_002389"
     CDS             5870..6625
                     /locus_tag="LZB08_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315885.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6625..7488
                     /locus_tag="LZB08_002390"
     CDS             6625..7488
                     /locus_tag="LZB08_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBM7315886.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7535..8014
                     /locus_tag="LZB08_002391"
     CDS             7535..8014
                     /locus_tag="LZB08_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBM7315887.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8079..9386
                     /locus_tag="LZB08_002392"
     CDS             8079..9386
                     /locus_tag="LZB08_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7315888.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYILVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9696..9836
                     /locus_tag="LZB08_002393"
     CDS             9696..9836
                     /locus_tag="LZB08_002393"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBM7315889.1"
                     /translation="MNGEGCILSNKITKECYKIGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            10348..11442
                     /locus_tag="LZB08_002394"
     CDS             10348..11442
                     /locus_tag="LZB08_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7315890.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11447..11647
                     /locus_tag="LZB08_002395"
     CDS             11447..11647
                     /locus_tag="LZB08_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBM7315891.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11647..12564
                     /locus_tag="LZB08_002396"
     CDS             11647..12564
                     /locus_tag="LZB08_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315892.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12561..13319
                     /locus_tag="LZB08_002397"
     CDS             12561..13319
                     /locus_tag="LZB08_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315893.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            13578..14651
                     /locus_tag="LZB08_002398"
     CDS             13578..14651
                     /locus_tag="LZB08_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBM7315894.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            14721..14984
                     /locus_tag="LZB08_002399"
     CDS             14721..14984
                     /locus_tag="LZB08_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315895.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQTMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
BASE COUNT         5832 a         1581 c         2795 g         4911 t
ORIGIN      
        1 aattaaaaat atacctaatt aaaataagta aagaaagagc tatcatagaa caaatttaat
       61 ttgctcatgc gacagctcct ttttagatga tgctttttta gatttatact ataaggtata
      121 taaaactaag cttattaaaa atgttataat ttctattgct atcatagacc atatcaatcc
      181 atcaagttca taaaagtaat taagctttat taaatagctt ctttattata atttctttat
      241 ttcatataat ataaatagta ttaaaatttt tatgggagtt aaagtaagca tgtattatta
      301 cttagatatt tgtataatca acatctagta atttatcttt ttggtgaaaa tgattaataa
      361 gttttttagg ataattcatt attaatttat ctggaaaatt aatttccttt aagatatttt
      421 cagaatactc atagttacct atgtcaaaac aaatgtgagc atctgaagtt agtatgattt
      481 tagcaccata ttttttgcaa agtgtagcaa tatacttaca gttatccgag ctaccatttc
      541 ttgacacgcc tttaatagaa caattattta tttctattaa gatattcttt tctatagcta
      601 atttaatgat taattcatag tcgattgggt agtttgggtt tccaaggtgt cctaatattt
      661 caatattatc atgcttattt attgcattta ttaaagcttt agtattattc tctaatgtat
      721 ttggagaaaa aacagcttcg tgaaatgaca agataagata atctaaccga ggaattacaa
      781 aaggctctag gtctatgttt cctttaatat ctagtatatt tgcttcacag cctcttaaaa
      841 ttataatgtt atttatgatt cttgggatat tttgaatgtt gtgaaagtac catctgtggg
      901 gagaaccagg catcttagga ccatgttcag atgtacctag tatcttaatt ccattttctt
      961 tacaataatt aatattttct agtaaagtgc tatagccatg accactgact atagagtggg
     1021 tgtgtaaatc tgataaaaaa ttcatataaa ttcctcctct ataaaagatg agggtaacgt
     1081 aatttatatt ataaaacgaa taggaaagaa aatcaatagt tacataatta gacaaaaata
     1141 ttaaaatatt gtagaattaa gcttaataat aaatgtttaa cagtattact gcttataggg
     1201 gaggttaact atgacacaaa agaagctatc aaggaaagaa aaagttttaa tttgcttgaa
     1261 aaggttaaca gaagaacact tgaactttaa taatggcatt agatttggat ttgatgcaga
     1321 atatatagct actgaagtag gtataaatag aagtaatgct agtaaggaat taaatcaatt
     1381 aataaaagaa agtattatta taaagataaa gggtaagcct gttcagtact tatataaaga
     1441 aagtattgag agtattttag gaaagaaact aaataaaggg atatttgata atattaactc
     1501 tataaacgaa tatagtgaaa atgattctat caaagatgag tatattttag agagtataaa
     1561 aaaagagaac aatattttta atttagtagg atgtgatgga agtttaaggt cccaaataga
     1621 acaggctaaa gcagcagtaa tatatccacc taaggggtta agtacattaa taataggacc
     1681 tacaggagtt ggtaaatcta tatttgcaga atatatgtat aagtatagtt taactataaa
     1741 agaagatagt gataatgcac catttatcat atttaactgt gctgattatt ctgataatca
     1801 gcaattatta ttagcgcagc tttttggata tgtaaaagga gcatttactg gagctgataa
     1861 agataagtat ggtattgtac atgaagctaa taatggagta ttgtttttag atgaagttca
     1921 tagattatct gcagagggac aagaaatgct atttctgctt atggataaag ggatatatag
     1981 aaggttagga gaggctaata aggtacacga gtgtagagtt atgataattg cagccactac
     2041 agaagaccca gaaacagcaa tgttggaaac gtttttaaga agaatacctg taactataaa
     2101 gattccgtct ttagaggaaa gaggctttca agagagaatg aagcttatat gttatttttt
     2161 taaagaagag agtgttagga ttggtgttaa gttaaaggtt tcaaaagaag ttattaaggc
     2221 atttatttta tataagtgta agggaaatat aggccaatta aaaagcgata tacaattaat
     2281 ttgtgcaaga gcttttttag attatatgac ctataaaagg gaatgtgtat atgtaaggct
     2341 ctctcttcta ccaaataata ttagagaatc attatatgga aataatagaa aagaggagtt
     2401 tgtacaaaat ttcagtttta ttggaaaaga tgatattatt tttttaccag aagaaaagga
     2461 ttttgaaagt gaaaatttat taatagaaaa taaaaaatat gatttagatt tttatggaat
     2521 gataaaagaa acttggaata aacttcaaca agaaggtatt aaagagtcac aaattagaag
     2581 tgttatagat ggagacatac agaagtattc ctataattta atgaatactt ttatatataa
     2641 tagtagtaat caaacagctt ataacaacat tgttaatgat tcaattgcaa ctactgtaaa
     2701 atatatttta caaaagcatg ataaatggag caaaagagaa ggattagata aattaataaa
     2761 agctatagct ttacatattc agaatttaat tgaaagaata aagattggaa atatagtaaa
     2821 gcatcctaat aaagatgaaa ttatgaagga aagagcatat gaatataaca tagcaaaaga
     2881 aatattatct aacatgtcat taatgtatgg agtagagttt ccagaagatg aagcaatttt
     2941 tttagcaaca tttctttatt tatcgtatgt gggattaaat gaggagagta tagcaatatt
     3001 agtcataatg catggtgaat ctacagcatc tagcatggtg aatgtagcca atactttact
     3061 ggattgtaac catgcagtgg caattaatat gggattagaa gatagagttc aggatatttt
     3121 agtacaagct gtagaaatag ccaataaaat tgataaagga aagggaattt taattttaac
     3181 tgatatgggg tctattttaa catttgctaa agttattcgt gatgctacag gaaaagaagt
     3241 aaaagcaata gatatggtaa gtacacctat tgtaatagaa gcaactagaa aggctctaac
     3301 tccagaaatg acattagaga aattatattt taatattata gaatctataa agaaacatta
     3361 tggagatatg gaagttacat atgctgaaaa agataatggt ggtagatatt ttgatagatt
     3421 actaattgat aatattagta aaactttgac ttttttaaat ggggaaaagg cttattttat
     3481 attaaaagaa gtaattaata gaattagtga acactattct ttagtaattc aagatgaact
     3541 tttagtaaag tttatatttc attgttcatg tatgattgaa agagtaatag taaaagagtc
     3601 tttagtttat aaatcatatg aagaaagaat tagtagaaat aaagaattat atttagttat
     3661 aaaagaaagt tttaagctag tagaagagac attcgatata attatttcag atatggaata
     3721 tgcaaatata ttagatatat ttgaatcaca atatgataca gataaattca aaagttgata
     3781 caaaatttaa aaatagccaa aaggctatct tttttttact ttttttgata cagatttgat
     3841 acaggttgaa aaaatctttt gatacatatc gaaataataa gtttttaaaa aaaattgagt
     3901 aaaaagtttt gattaaatcc tgtaaaatag ccatttgtta ttgatacaca taagttggca
     3961 cggcaattgc ttatataaaa agtgacaaga aaagtgaggt gaaattggtg ataggaataa
     4021 tagttatgac tcatgggagt tttagtgaag aaattataaa aagctgtgaa cttattgcag
     4081 gaccagcaga aagaacagca gcaattaaac taaataggaa tgataatatt gaagacttaa
     4141 ataaaaattt tgtagaaaag ttaaatgaat tagatgaagg tgacggagtt ttagtattag
     4201 ctgacctatt aggaggttcg ccaagtaatg ttgcaagttt aaaccttaaa aaaggtggca
     4261 agtttcatgc acttactgga gtaaatcttc caatgctact tgaagcttta attaatagag
     4321 atggaaaaag tttagaagag ttagcagaag catgtattga agcaggacaa actggtataa
     4381 acaatattaa taaagttctt agctcaatgt aatagaaaaa ggagtttgat taaaaatgtc
     4441 tttagtaaaa tattgcagat gtgatgatag attaatacat ggtcaagtta tatataaatg
     4501 ggttaagcat ctaggtgtaa aaaagatagt agtagtagat gatgaaacaa ccaatgatgt
     4561 tattgctaaa ggtttgataa aaatggcagc accaaagaat attgatttat cgatattaac
     4621 tgtaagtgaa tcaaggagat atttttataa taatcaagct gatgataacg tttttgtatt
     4681 aattaaaaat ttagatactg caaatagaat gatagaagaa gggattaata tcaaaaaact
     4741 tattgttgga agaataccta caggaatagg aaaaaagaaa atatctcaaa atgtatacat
     4801 taataaaaaa gagtttcttt taatagatga atttattaaa aagaatatta atgtatcaat
     4861 tcaaatggta cctgatgaag aagaagtaga attaaaccaa aatattaatc gctataaagg
     4921 agagtttatt tagcaatgaa taaatataag gtaatcatac tgtgccatgg aacttgggga
     4981 aaagtcttag tagaagaaac aaataaaaac tttggattgt caaatcaata tgaagtttta
     5041 tcactaagtt ctgaaaaaga tgtttctgta tttatgaaag aggttgaaga agtagtagat
     5101 aaagaagatg tgaaaattat aatatcagat ttatatggag gatctacatc aagtgttgca
     5161 atagctgttg ctataagaag aggaatagat gcaataaatg gacttagctt acagacaatt
     5221 ttaatcgtag atgaagaatt aagcaaagaa gataaattac atacattacc agaaagaatc
     5281 gtcaaaaaaa ataataacct ttgtgtggat ttaattaaag aatttaaaaa aatataaatt
     5341 atgaggtgaa tataatggca aaaataacat tagtaagagt agaccacaga ttaattcatg
     5401 ggcaagttat aacaaaatgg gtaaaaatcg ctcaagctca aaaaataata atagttgatg
     5461 acttcttagg tcaagatgaa tttatggctg acatatataa aatggcagca ccatcaggag
     5521 tagaagtagt tattttaaca gcagaagatg cgggacaagc attccaaaac aacactttag
     5581 gagataaaaa tatatttatt ctatttaaaa atgttgatat ggcaaataag gcttataaag
     5641 ctggacttaa atatgaaaag attcaattag gaggtattcc aaatgaagca ggaaagaaaa
     5701 tggtgtttac agctgtttca ttaggaaatg aggatgtaga acaacttaat gaacttaatg
     5761 aaaatggggt tgaaatagtc ctacaagtta taccagagga atcatctatg acatatgaaa
     5821 atgcgttaaa gaaatttaaa taaaaaatat aaattaggag gaattataaa tggaatctgt
     5881 tttgatgttg gcaattgtaa caggattatg gtattggttt gcagcaggtc ttgcagggta
     5941 tacccttttc tcaacactaa agtctccttt atttataggt tttagccttg gattactttg
     6001 gggtgatgta acaactggta tgattgttgg tgcaagtata gaaatggttt atctaggaat
     6061 ggtcgcagca gggggaaata ttccatcaga taaatgtcta gctgctctta ttgcaatacc
     6121 agtagcactt caaactggtg ttaatgcaga agttgcagta tctattgcgg tacctttagg
     6181 tgttatagga gtattagtta ataacttaag aagaactggt aatgctgtat tggttcataa
     6241 ggcagataag tatgctgaag aaggtaatac aaaaggcatc tggagatgtg caacacttta
     6301 ttctttaata tttgggtttg ttttaaggtt tccaatagtt tttgtatgta acttttttgg
     6361 agcagattta gttcaatcat tattagatgt tataccacaa tggttaatga atggattaac
     6421 agtaatgggc ggtatattac ctgcattagg ttttgcaaca acaatattta ctataggtaa
     6481 aaataaattc ttaccaatgt ttattatagg attttttatg gttcaatatt ttgaaatatc
     6541 aattactgca gctgcaatat ttggtgtttg tatagctctt ttaattacct ttatgaaaga
     6601 agacaaaagg gtaggtgaag tataatgcaa gaaataaaaa ataaagaagt taaaaatgga
     6661 caaggtccaa ctgaagttaa taagacaaga gtacttacaa agaaagatgt aacaaaaaca
     6721 tatctaagat ggtggtggac agcagaatta tccaactctt ttgaaagaat gcaagcatta
     6781 gcagtttgtg caagtttcac tccggcttta gaaaagttat ataagagaaa agaagattta
     6841 gtagatgcgt taaagagaca cttacaattt tttaatactc aagctatatg gggaggatta
     6901 attcacggta cagttttagc tatggaagaa gagaaagcta ctgaaggaaa aatcccagga
     6961 gaagttattt caggtgttaa gaatggtctt atgggacctt tggctggaat tggagatact
     7021 ttagattttg gtacatttca aacaatattc ttagctttag gtgcttcatt tggagcagaa
     7081 ggaagtgtta taggtgcatt tttcccaata atgtttagta tattactatt ttgcgaaggt
     7141 tattacttat ttcatttagg atattcttta ggtagagatt caattaagaa aattctatca
     7201 ggtggtattg tcaataagat aattgatggt gcttcaattc taggaatgtt tatgatggga
     7261 gcattatctg caactacagt taagctttca actccgctat catttgatat tggtggtaaa
     7321 gcaattgttg ttcaagatac attaaatatg attgctccag gtttattacc acttggtgtt
     7381 gtattcttcg tatattgggg aatgaaatac aagaagtgga caataactaa gttattagta
     7441 attttagttg tattagcatt ggtagggtca ttcattggaa tattctaatt cacagtgatt
     7501 aaaatataga atttaatttt aggaggagaa agaaatgtca gaaaaaatgt atacagaaaa
     7561 ggaatattta gaaggaatta gaggagcaac aggagaaaga gcagtatggt tctatcttct
     7621 tatgaaagaa gctgaaaaat taggagctaa tccagatgat atatgtaaag aagcaatata
     7681 tggatttggt aaaatgagag gtcaaaaata taacgtagca gatactccag gaaaaatggc
     7741 agaaatgtta tataacagta aaggacaaaa agtgtttgaa atggaattag tagaaaatac
     7801 agatgagaat ggcgttttaa aattccatca ctgtccatta gatgctgctt ggaaagaata
     7861 tggattaact aaagaagaaa gaaaagaaat ttgtagatta gcttgttatg gggattatgg
     7921 aagagttgac tgtgcacaag gtgttaaatt agattttgct caaaaatgtg ctcatgatga
     7981 tgaagtttgt gaattagtat ttacaaaaaa ataattttta atgctaaaag cctgttcttc
     8041 aaggacaggc tttaaaaaga tagaaagtag gttgatttat ggatataagc agaagtgtag
     8101 ataatagttt aaatagaacc atagaatttc taaaagaatt gataaaaatt gattcacaac
     8161 aaggtgaacc tatttcacaa tgtccttttg gtataggacc taaaaaatct ttagataaaa
     8221 ctttagatta ctgtgctagt ttaggtttta gtgttaaaaa tattgataat tatattggat
     8281 atgcagaaat tggagaggga gaggaattaa tagggattcc aatgcattta gatatagttc
     8341 caccaggaga ggggtggagt gtagacccat tttcgggagc agtaattgac aatattattt
     8401 atggaagagg tgtcatagat aataagggtg ctgtatctat gcttatacat gtattgaaaa
     8461 acatagagga tatgtatcct acaattaata aaagaataag attaattttt ggtacaaatg
     8521 aggagacagg tatgaaatgt atcaaatatt acttagataa aggtgaagaa attccaagta
     8581 tgggatttac tccagatgct atgtatccag tggttaatgg agaaaaagga agggttcata
     8641 taaggataga aaaagaaatt aaaattgaca aaagtaaacc ttacatatta gtaaggggag
     8701 gaactaaaga aaatgttgtt ccatctcatt gtactgcaaa aattattaat ggaattattt
     8761 cagagttaac tactaaagga gtagctgtac atgcaagtaa tcctgaaaag ggagaaaatg
     8821 ctatatctaa aatggtaata aaaatagttg aagataatat ggattttcag catagagaag
     8881 atatagaatt agtttcaaaa tacctttgta gtgattatta tggagatgca ttggggatta
     8941 atcaatatga tgaggtattc aaaaacacta ccttaaattt aggaatatta aaagtaaatg
     9001 aggaaaaaat cgtatgtgag ttagatataa ggtatggtaa aaatatagtt ttaaataaca
     9061 taatagatag attcaaaaag gttttttgta atggttggaa aatagaggtt attgctcata
     9121 aagacttaca ttatgtggat gagagtaatt tagtacttaa aaaattatta gaagcctatg
     9181 aagaagtaac agatgaaaat ggatatacaa ttgctatggg tggtggcaca tatgcaagtt
     9241 ggtttaaaga tatggtggcg tttggaccta agttcttagc gtataaaact ggtggtcatg
     9301 gagtagatga aagagtacca ataaatcata tacgaaagaa tatggagata tatactttgg
     9361 ctttaataaa actattagaa ttgtaaaaga agaagagact ttatgtgcaa atttacagat
     9421 atcttaaaaa gagagataaa acgagttcaa ccttatttaa atgttcaaca ccatcaaatg
     9481 gggctgtctc aaaatagaga taagcctctt acttattgtt gaaatgatga aaatatattt
     9541 tacttcatac aaaaaagagt gccttatgaa gtaaaaagtt aattttgaga caccctcatt
     9601 tgatgatgtt gaagagaaaa taagtatgaa attattaaaa aagatgattt tataaaagtt
     9661 aaggttgaaa agaaaagtta attaaatgga attagataaa tggagaagga tgtatactat
     9721 ctaataaaat aacaaaagag tgttataaaa ttggttatat gattagagaa aagtctattg
     9781 atacttatcc agatagtgga tggggattta tgacttggga tgaaaatgga gaataataaa
     9841 aattatagtt tatacagttt aataagatat atgtacaagc agttaatcta aatgattgac
     9901 tgcttttctt ttatagattt ttatataata aacaaatacc aatacatgat agttgcctgt
     9961 tataattaga ggaataaata cgaataataa ctttataaaa gaattaaatg gaaaatcgaa
    10021 gagttatatt cagtatttta aaaagaatat gaaatgagaa atgaaaataa ggtgtttata
    10081 ttaaaagagg ttttatacaa aatatttaaa aacgtgataa ataaaaactg tatttagata
    10141 gattaactat tagttttata tataatatgg attttatttg caaagtttac atattttaca
    10201 aaagacatta aaaataaatt taaatatata ttttatatac ttgtttattt gtttctctgt
    10261 ggttgatttt ttcgtcaaaa gtcaacttca aatcgcttgt tagctttaca tgtttaagga
    10321 tataattata cataaggagg gattgacatg aatgaattaa acatcgcaaa aacgctgatt
    10381 ttaaagcgta aagaaaaagg aattacacaa gatgaacttg caaattatat aggcgtttct
    10441 aaagcttctg tatctaaatg ggaaactgga caaagctatc ctgacataac ctttttaccg
    10501 caactcgcga catatttcaa cattaccgtt gatgagttaa tatgttatga acctcaaatg
    10561 atgaaggaag atattaataa actgtataat aaactgtgca aagactttac ggttaaacca
    10621 tttgatgaag tcatggatga aataagagaa atcataaaaa aatattattc atgttttcct
    10681 ttgatttttc ggatgggtct tttaatagtt aatcactatg atattgtaga tgaaaaaaag
    10741 cgtgaactat taattagtga agcattagaa atatttattc gtatacagga aacttgtaat
    10801 gacatagata tttgccgtca agcaaaaagt atggaggcaa cttgttatat attgttaaat
    10861 caacccatac aagtgattga tttattgcaa aacagcaatt ttcccatgat aaatgaatct
    10921 atacttcttg cacaggggca aatgatgaat ggtcaaatgg atgaagcgag agaaacattt
    10981 caacttggtg catatcagaa ccttatatca ttagtccaaa atctagtggg tattttgcaa
    11041 aatgcagata aattacaaat gaaggaaata gaaagacgta ttctagcaat ttcagatata
    11101 tttgagcttg atacattatc tcctgctatt atgctttctt catatttaac acaggcacaa
    11161 attaatttaa tacatggtga taatgaagga gctattaaat cattacgaaa atatgtagat
    11221 ttagcaacac gtgatatata cccaattata atacatgggg atgacttttt taataaactt
    11281 gacagatgga tttctgaaat tggaacaggt attacacgtg atgatacaat cgtaaaagca
    11341 ggtattgttg ctgctattaa aaataatcca atgttttctg tattaagtga aaacaaagaa
    11401 tacaaatttc ttatagaaaa attatcttta ttggaggaat aagattatgg aaaatttgat
    11461 ggaatactta ccacttttgg ttcctgttat tattttagat ttaatactta taattactgc
    11521 acttgttcat gtgttgagac atccaaatta taaaattgga aataaggcaa tatggattat
    11581 tgtagtactg tttattagtc taataggacc aattttgtat tttacaatag gaagaggtga
    11641 agaatgatga atattgtctc aataaatgga ttatcaaaag gttttggtaa tcgaaagata
    11701 atagataatt taaattttac tgtaccagaa gggtcagtct ttgggtttgt aggtaagaat
    11761 ggtgcaggta agactacaac aatgaaaatg gtacttgggt tacttgaacc agatagtgga
    11821 actatagatg tttgtggtga gaaagtgacc tatggaagaa caagttctaa tcgtcatgta
    11881 ggttatctgc ctgatgttcc agagttttac aactatatga gaccacttga atatctttct
    11941 ctttgtggtg aaattacggg actttcaaaa aaagaaatac agataaggag tgaagagttg
    12001 ttatcacttg tgggtcttgg aaatgaaaag agacgaatag gtgggttttc tcgtggaatg
    12061 aagcaacgac ttggtatagc ccaagctttg ctttcacgcc ccaaattact tatttgtgat
    12121 gaaccaacaa gtgcacttga ccctgttggt cgaaaagaaa tattagatat tatgcttaaa
    12181 atcaaagact caacaactgt aatattttca acacatatac tatcagatgt tgaaagaatc
    12241 tgtgaccatg ttgctatatt aaacaaagga agcattgccc ttagtggtac actttcagaa
    12301 ataaaaagta tgcacggaaa ggatagactt ttactggagt ttgcaagtaa tgatgaaatt
    12361 caaaagttta agtctagtga tggtattaaa tccttactaa aagattcaga agaaactaat
    12421 atggaaattg ttttgcatgg taaagatata aaagcaatac aaaaaacagt tatatctaca
    12481 ctcgcagaaa tgaatctttg tccagtaaag atggaactaa tagaattatc acttgaaaat
    12541 ttatttttgg aggtagtaaa gtgagagaat acatagcatt tacaaaaaaa gaatttaaag
    12601 agaatttaag aaattataaa ttatttagtt taattatttt atttctaata tttggtataa
    12661 gtagtcctat ttctgcaaaa tttatgccag acttgattgc tcattttgca ccgactttaa
    12721 aagttactgc tgcaccaact gctttagatt cttggacaca attttttagc aatatctctg
    12781 gtcttggtat gagtttaact ctaattattt tttgtaatat tttatcaaat gaatattcta
    12841 aagggacact tgttattatg ctgactaaag gtttatcacg ttcatctata gtattgtcta
    12901 aattctctgt tacggtgatt attatgacaa ttggtttttg gttgagtttt ttatttgctt
    12961 atggctatac aatgtatttt tggccaactg caaatcttaa tcatattata ttttcagcat
    13021 ttaatttatg gcttatagga attatgtata ttagtgtatt aattcttggg tgtgtactat
    13081 ttagaccagc atttgccagt gttttacttg tattggtggc aactctttta ttgagtttaa
    13141 tttctattcc taaacagatt gcaccttata ctcctaattt cttgatgtca aaaaatatag
    13201 acttgatttc agggaaagtg gcaatgccag aatttattat tccaattatt ataacagttc
    13261 ttatttctat agtatgttta ctagcagctg ttattttatt taataaaaaa ccagtttaat
    13321 aataccttta tcacaaggta aagtaatctt atgttgttaa aaactacacc taaaaatcaa
    13381 aaagacttgg acttaagttt caagtctttt tgatttttag gtgtagttta atatatagaa
    13441 ttaagtgtag aaaataatac aaataatatt atttgtatca aataaatata agaatattta
    13501 attatgttaa aatatactaa aaattacttt aaaagtgata aaattagtat aagtaaatat
    13561 tttaaagggg tgtagatatg gaaaagtcaa aagtatattt ttgtgattta tactcaaatt
    13621 cgcaaaataa aaacgtacca aacaatgtaa gaagattatt tgatgaagca ggattcaaag
    13681 atttaataga aaagaatgac caagttgcaa ttaaacttca ctttggagaa aagggaaata
    13741 ctacatatat gagtcctgtg gcagttagac aagtggttga taaggttaaa gactgcgaag
    13801 gaaagccatt tttaacagat acaaatacac tgtatacagg gagtagaaca aattctgtag
    13861 accatcttac aactgctata gaaaatggat ttgcttatgc agttgtaaat gcaccagtaa
    13921 taattgcaga tggattatat agtagaaatt atgagaatgt aaaaatagac aaaaaacatt
    13981 ttgaaagtgt aaaaataggt ggagaaattt ataattcttc tgcaatgata gtaatgagtc
    14041 attttaaagg tcatgaagca gcaggttttg gaggagcatt aaaaaactta gctatgggat
    14101 gtgcaagtgc agcaggaaaa caaatgcaac attctgatgt tactccagta gtaaaggaaa
    14161 agaagtgtgt aggttgtgga aagtgtgtta attcttgtcc tacaaaagct ataagtatag
    14221 tggataaaaa agcagttata gattctgatg tatgttatgg atgtggagaa tgtccaactg
    14281 tctgtccaac tagagcagta actatacaat gggaaagtga ctcagatgtg tttgtcgaaa
    14341 agatggcaga atacgcttat ggagcagtat caaataaaaa agacaaggta gggtatataa
    14401 cttttgttat gaatgtaact ccactttgtg attgtgtacc ttggtcaggt agaccaattg
    14461 cccatgatat aggaatatta gcatcaacag accctgtagc tattgaacaa gcatgctatg
    14521 atttgatttg taaggaaatg ggtcatgatg ttttcaaaca tgaacatcct catgtaaatg
    14581 gaactagaat aatagactat gcatgtgaaa tgggtatggg aagtaaagaa tatgaattaa
    14641 taaaattata agaatagtag cacttaaatg catataatta ttgtaagaga aatctaaaag
    14701 tatcaaagga gtatgcattt atgaaaattt actatgagaa ggaaaacagc aagaaggcta
    14761 atgtaaacaa tattccaagt gtatttgcgt ttaattttct aagaaacttt ttgactagca
    14821 aagagtatga ggaaactaga aaagaatatt ttataaataa tctaacaaaa agtcaggtta
    14881 atcaaaccat gaaggattta aaatggctat ttagagaata tgaaggctta gaggttatta
    14941 caatggaaag cttggatgga gttaagacaa ggatagtttt ataaaccagt aagaattata
    15001 ttctaataaa aaatactgct ttaataataa tatgtataat ccatttacta atattatttt
    15061 gaagcagtat ttttttgtgt atagtaaact tgtgtctaaa ataaagttga ccaataaat
//
LOCUS       DAFKMX010000103        14669 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFKMX010000103 DAFKMX010000000
VERSION     DAFKMX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.103"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(149..1039)
                     /gene="metA"
                     /locus_tag="LZB08_002400"
     CDS             complement(149..1039)
                     /gene="metA"
                     /locus_tag="LZB08_002400"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBM7315896.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(1069..2334)
                     /locus_tag="LZB08_002401"
     CDS             complement(1069..2334)
                     /locus_tag="LZB08_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBM7315897.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIEQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2444..2546)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2600..5257)
                     /locus_tag="LZB08_002402"
     CDS             complement(2600..5257)
                     /locus_tag="LZB08_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBM7315898.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKNALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(5385..6128)
                     /gene="yaaA"
                     /locus_tag="LZB08_002403"
     CDS             complement(5385..6128)
                     /gene="yaaA"
                     /locus_tag="LZB08_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBM7315899.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6138..6602)
                     /gene="bcp"
                     /locus_tag="LZB08_002404"
     CDS             complement(6138..6602)
                     /gene="bcp"
                     /locus_tag="LZB08_002404"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBM7315900.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKIVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(6954..8651)
                     /gene="ade"
                     /locus_tag="LZB08_002405"
     CDS             complement(6954..8651)
                     /gene="ade"
                     /locus_tag="LZB08_002405"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBM7315901.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(8957..10111)
                     /locus_tag="LZB08_002406"
     CDS             complement(8957..10111)
                     /locus_tag="LZB08_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBM7315902.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(10356..11195)
                     /locus_tag="LZB08_002407"
     CDS             complement(10356..11195)
                     /locus_tag="LZB08_002407"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBM7315903.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(11208..11804)
                     /locus_tag="LZB08_002408"
     CDS             complement(11208..11804)
                     /locus_tag="LZB08_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBM7315904.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(11855..12505)
                     /locus_tag="LZB08_002409"
     CDS             complement(11855..12505)
                     /locus_tag="LZB08_002409"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBM7315905.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNAKEVIQIAENSDIDFKDNNIYLDEEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(12551..13804)
                     /locus_tag="LZB08_002410"
     CDS             complement(12551..13804)
                     /locus_tag="LZB08_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBM7315906.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(13828..14469)
                     /locus_tag="LZB08_002411"
     CDS             complement(13828..14469)
                     /locus_tag="LZB08_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBM7315907.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
BASE COUNT         4594 a         2643 c         1583 g         5849 t
ORIGIN      
        1 acatacacct aaagtgattt tcttaaaata taaaactact attataaggt caaattttaa
       61 aaaaataagg tataaagtat cattaagatt aactttatac ctataaattt ctatattttt
      121 ttagttaaca tttatatact cttaaaattt attgtatcaa atagtttttt atccaatttc
      181 taaacaacaa ttctgaatgt tttttccatt ttacagttgg ctcttctgaa gggtcatcat
      241 taatatagta attttgaggt atagatattt tatctccttt atttatatcc ctcttatatt
      301 ctttatcaag agtgtatttg tcatactcgc tatgacccat tacgaacaca tctcttgaat
      361 ttgtaattat atagggacca gcttctttag aatgactcac aatgctcaac tctttattct
      421 ctttcaaagc ttcaatattt acagtagtat gtcttgaatg tggagcaaaa aattcatttt
      481 caaatccatc tactatttta ctatctttat ctaccttatg cttaaatact ccaaaacatt
      541 tttgacttag tggaagtttt tttacattgt agtatttgta aagacttgct tgagctgccc
      601 aacatatata taatgtagat ttagtttttc tcttagaata atccataatt tcttctagtt
      661 cattccaata atcaacttct tcaaactcca tttgttcaac tggagctcct gttattataa
      721 agccatcaaa atactctcct ttggcttcat caaatgtttt ataaaattct ttaacatatt
      781 cattacaact tcttttactt tctcttgttg aaattttaat aaattccaca gacacgttga
      841 atcctgtttt atctatcctt cttagtaaat ctaattctgt atctatcttt ataggcatta
      901 gatttagtat agctatctta gtgtctatgt tttcttcata ctttaattcc tttttaaaat
      961 cttctttata aattacatct attccttcat ctaataattt attaataaca ggtaatccct
     1021 ttggtagtat taaagccata tcttttcact tctctttctc ttagaatatt agaaatttaa
     1081 agcttgattt aaatcagcta ttaaatcctc tacattttct attccaacag ataatcttat
     1141 tagtgaaggc aatactccag atgcaattaa ttgttcttct gttaattgtc tatgagtagt
     1201 tgaagctgga tgtataacac aagttcttac atctgaaaca tgtgtaacca aagatgctat
     1261 ctgtaatttt tccacaaatt ttttggcatt ttctaaccca cctcttactc caaatgataa
     1321 aacaccacta catcctcttg ataaatactt tttggcattc tcataatact tgttatcttc
     1381 aagtcctggg taattaatcc aatctacatt ttcatgtttt tctaaaaatc tagctatttt
     1441 aagtgcattt tcactatgtc tctccattct aagatgtagt gtttcaacat ttaaattagt
     1501 aagatacgca ttgaatgggc ttaaacaatt tccatagtct ctaagcaact gaactcttgc
     1561 tttagttgca tatgcggcat ttccaaattt ttgagtatag cttattccat gatatgttgg
     1621 gtctggctca actaattctg gaaattttcc attatcccag ttaaagtttc caccatccac
     1681 tataattcct ccaacacttg aagcatgccc atctaaatat tttgtggtag aatgagtaac
     1741 tatatttgct ccatacttta aagggttaca aagcactgga gatgctaatg aattatcaac
     1801 aataaatgga acattaattc tcttagctac atttgctatt ttttcaaaat ctatgacttc
     1861 aagagttgga tttgcaagtg tttctgcaaa cacaacctta gtattttcct ttgcaagttc
     1921 tactatctca tcttcacttg aatctaagtc aaacgatatt aaatctatac caaatttttt
     1981 aagactaggt cctagtaaat tgaatgttcc tccatatact tttgaagaac aaagtatact
     2041 atctcctgat ttacatatat ttaaaactgc caacatattt gcagattgcc ctgatgatac
     2101 agctacagaa gatactccac cctctagtaa acttattttt tcttcaaaag cttgaatagt
     2161 aggattgctt attcttgaat atatatgtcc atcaactttt aaatcaaata attcagcaag
     2221 ttgttctata ctgctatatt taaaagttgt actttgatat aaaggtagta ctcttggttc
     2281 tccattacct ggtttataat ttccttgcac acatattgtt tctttattat acattcttgt
     2341 atccccctta gattttagta aaaaaaatta aactatttac cagatgagag ttaaaataaa
     2401 taaagcctga gtatataact caggctctgt ataagcttaa ttatactcat ctctcagctt
     2461 tcgccgcagg atttagcacc aatgtatata aatatactgg ttgccgggtt tcatagggcc
     2521 agtccctcca cctctctgga taagatttat ttaatttttc tatgttttat atcttatata
     2581 tttttcctaa ttttgtcaac tattttttta atttttttct tttaataatt ttcccaacct
     2641 ctacttgaat tacagacatt atggctaaaa caatcgttat aagccactct aagttattta
     2701 aaactgttaa gttaaaagca tttctaaaaa caggtacaaa cagtattact gctattaaaa
     2761 atgcagaagt taaaaatgag attaaaagaa atggattttt accattattt ctattaaaca
     2821 ttgaatctgt atttgaacgc tgactaaatg ctcgtaacat ctgtgaaaat gctaatacac
     2881 aaaatgccat tgtttgacct acagaatgat tttctatatt aagaccaatt ttatatgcta
     2941 aaatagtcat tattgccaca aatatacctt gggtaacaac tctttgtata agattttttt
     3001 caaacaaagt ggttgatttc actggctggt gcttcattat atttctgctt ggtgggtcaa
     3061 ctccaagagc aagggctgga agcgtagcag ttgcaaggtt tacccataga atatgaacag
     3121 caagaagcgg agtatcccaa tttaataata ttgctacaaa caacgtaagt atctctgcta
     3181 tattaccaac aagcaaaaat tgtataactt tttgtatgtt tctatataca cgtctacctt
     3241 cttttattgc ataagcaatt gttgtaaaac tatcgtccat taaaatcatg tcagaagaat
     3301 cttttgcaac atccgtacca cttataccca tggctatacc aatatcagca gactttaagg
     3361 caggagagtc atttacacca tccccagtca ttgctggtac ttctccaatt ctttttagag
     3421 attgtataat tctaagttta tcaaatgggg atacacgagc aaatactgta gtatttttaa
     3481 cagcttcatc tagttcatca tcactcaaat tatcaagttc atcaccagat atcacagtat
     3541 tttcttcact ccatattcct aactcatgag cgattgttgt ggcagtaact ttatggtcac
     3601 ctgttatcat tatggttctt atacctgctt ctttacaagt acttactgca tcaattacct
     3661 cagttcttgg tgggtcaatc ataccaacca tacctataaa tgttaaatca tattctatat
     3721 tttcactatc ttcttttggt atagatgaaa tctcacgctt agcaaaacca agtacacgca
     3781 atgcattttt agacatgtta agacataatt cgtgtatatt ctttttatcc acttcagtaa
     3841 tgtttctttc accttttgaa gttaatattt tagtacatag gtcaagaatt tcgtctactg
     3901 ctcctttagt ataagcaata atcttttcat ctatattatg aacagttgtc atacattttc
     3961 tatctgaatc aaaaggctgt tcaaatacac gtggatattc ttcttcaaga gcttcttgat
     4021 ctattccaaa ttttttggcc ataaaaatta atgcgccctc tgttgggtca cctaatattt
     4081 catctgtttt atctggatta atacatgcat cattacaaag tgcaccagca taaacaatct
     4141 ctttatacac taatggatgt ttttcagatg cagtatctat tgatgtcact tttccagatt
     4201 caaaatctcc attgacagca atatgtgtaa cagtcatttt atttaaggtt aatgtaccag
     4261 ttttatcaca acatattact gtagctcctc ctaatgtttc aacagcaggt aattttctaa
     4321 ttaaagcatt tcttttagcc atacgctgta cgcctaaagc cattacaata gtagctgttg
     4381 caggtaatcc ctctggaata attgatatgg caagggaaat tgcaatcata aacattggca
     4441 agagaggtct ctgataaaat gcacctattg caaatataac aacgcataca ataataccta
     4501 tgacacttaa tgtctttcca acagtattaa gtttacgttt tataggagta tcaaaatcat
     4561 cttgtgaatc taaaagatgt gctatgttac ccacttctgt atccatacca gttgctacta
     4621 caacacctac acctctacca tatgtgacta ttgaagatgt atacgccata ttgctacggt
     4681 ctcctaatac acattcttta ggtaagattg tatctgcatc tttttctgat ggaacagatt
     4741 ctcctgttaa agaagcctct tgaattttta aacttgatgt ttctattaaa cgaaggtctg
     4801 ctggaaccat agctccatct tctaaaaaaa caatatctcc tactacaaga ttacttgctg
     4861 gaataacatt ttcttcacca tcacgcataa cacgagctgt aggtgaattc atatttttta
     4921 atgcttctaa tgatgattct gcttttttct cttgaacaat tccaataact gcatttactg
     4981 ttacaattat tagtattaca aaagcttctg taaattcgcc aaatatcaaa gataatattg
     5041 ctgctcctat tagtatcata accatagcat ctgtaatttg actccaaagc atttgtaaag
     5101 ttgttctagg tggttttttt ctaagttcat ttaaaccatt ctctttaagc cgtttttccg
     5161 cctcggtatc ggtgagcccc tcctctgatg aatctagact ttctaaaatt tcatgcgttg
     5221 ctcttgaatg ccaaggcatc tgtttgtgat tttccatttt ggaagaccac tcctttcttt
     5281 ttttaattta agaataatta tattttaaaa tcaagttcaa ttcaatacac actaagtata
     5341 ttttacctaa atttaacaat gccatttaat atttaacaaa acttctactc ttttacaaat
     5401 atgtattcac tattgttaga taagttttct ttatacttat aaccttctaa attaaacttt
     5461 ttcagttctt caatatctct tatcctattt tttacaatgt attttgtcat catacctcta
     5521 gctttctttg cttgtgtaga ttccactttc aatatgccat ttttatttac tttaaaagta
     5581 catgtaatat atgtatcact atctttaata aatttttcta tggatttgct gtattcattt
     5641 gatgaaagat taacgatagc tgttttttct ttgattagtt ctttatatat acgatttccc
     5701 caatattcgt ataaattctt aaactcacct actcgtaatt ttgtctgcat ttctaatcta
     5761 tattcatata tactatcata tggttttaca acaccataca atccacttat gattcgaata
     5821 cagctatttg caaactcttc atctttcctt gtaaaatttt ctgcttctat atttttatat
     5881 tgtattccat cataagttaa gattgcaggg tttcctaatc tatcaaattt aatgctttca
     5941 aatctatttt tatttaatac agctaatttt tcattaattt tcattttatt tttaaaatct
     6001 tctataccaa atgtctttat attttccact atttcttttg tgttatctat aaaacaaggc
     6061 aaactcaaat ctctatcaaa tacttctata ttcttcatat ttttagctgg tgataacatt
     6121 ataatcataa attcttatta ctcctgtttt tccaaatatt caagaatctc ttgtgcattt
     6181 gtgtctggtt ttgctttttc aaagactttt tcaataattc cattttcatc tataatatat
     6241 gtcgcacgta caactcccaa tgcagttttt ccatacattt ttttctcttt ccatacatca
     6301 aaagctttta tagcaactaa gtcagggtca gaaagtaaaa taaaaggaag ttcatgcttt
     6361 tctgcaaatt tttgatgtga ttttatacta tctttactta tcccaataac ttgtatatct
     6421 tcctttttaa aaccatcata tgcatttcta aaagcacaag cttgacgtgt acatcctggt
     6481 gtattatctt taggataaaa atatactact atttttttac ctttaaaatc tgacatactt
     6541 accttatttc catctttatc ttctagtgta aattctggtg cttttgttcc tatactcaac
     6601 ataaatatct ctccttttgt ttttattaat aattttatac tatgcttata ttattaccct
     6661 ataaatctat atataaataa aatattttat tttttatgta taaagtttca taaatatata
     6721 attttcatgt atttatatgt taaaatagac tgaaaatagt tttattaaga tatctatgct
     6781 taaaacaagg ttaaatgata gattaattaa aataaggata tctaaaaata aaatttttag
     6841 ttcatgagat gctctttttt gtatgaaatc aaatatattt tcatcatttc aacaataaaa
     6901 agagacttat ccctattttg agaaagcctc atttattatt acttttatat tatctatata
     6961 aatttaaatt cctcaacatc aaatagacct aaatctgtaa gtcttatttg tggaacaact
     7021 ggaagtgcca taaaagaaag tgtaataaaa gggtctacat actctggaac tcccattttt
     7081 cttgctatct ccaacatatt atcagtaatt tcttgtactt cttcagctgt aaaagtacta
     7141 attaatccag ctacttgaag acttaggtgt gcaagaactt ttccatttga tacaattaca
     7201 taaccacctt gtatttcctt taagtaattt atagctacta gaatatcatc atcattatcc
     7261 cctgcaacta taatattatg agaatcatgg gccacagtag tagcaattgc tccatttttt
     7321 atgccaaatc cttttagtgg agcaattgct acatttccag tcattctatg tctctcaaca
     7381 acacaaagtt ttgaatactc tttatttgga acaaaatatc catctgcaca tggtaaagat
     7441 tcatataact tatttgtcaa aatttgataa ggtacaatct ccattacata atttttttca
     7501 cttactttta actgtatttt ttctttatta atatatttaa acttgactgt attaagcaat
     7561 tctttatctt taatctcata attgtaatta gaaaacatct cttcgtttac caaattacca
     7621 tctttatata cactatccac ttccattttg tctaaatcat tcaatacaac aatatctgcc
     7681 ttataacccg cacctattgc tccaattttc cttaacccat atgcttttgc agaattataa
     7741 gttgcaactt ttatagccct aacaggttcc atacctaaat caatcgcaca ttttatattc
     7801 catcttatgt gaccttgttt ttctatatca tctaagtgct tatcatctgt gcaaaacata
     7861 aattcttcaa ttggcaagtt atgctttagc ataccactta ctatagactt taaatttttt
     7921 gctgcgcttc cttctcttat taaaattttt aatcctgctc tcaatttttc atatacttca
     7981 tcaaaagtta tacattcatg gtcattttct attcctgcac atacatatgt ctgaagtgat
     8041 ttaccattta tattaggtgc atgtccatct acaactttat ttttaaaaag ctctagttta
     8101 tccaagattt cattttcaga atttataaca tcattaaaac acattacctc tcctaaacct
     8161 aaaattctag gattatttac ataactctcc atgtcttttg cactaaattt accaactcca
     8221 ttagtttcaa aacttgttgc aggtacggat gaaggcacca taatatatac attaagtggt
     8281 atatcttttg tcgattctaa taaaaaatct attgcactag ctccttttac attgaccaac
     8341 tcatgtgggt cggctatgat agttgtcgta cctgatttta ataatttttt cgataattca
     8401 attggcatta ccattgtaga ttcaatatgc atatgtgcat caataaagcc aggtgatata
     8461 tatttgttac tacaatcaat ttctacattg cccttgtaat cgccaattcc aacaatagtg
     8521 tctcctacta ttgctacatc tcctgttatt aactcttctg taaatacatt tatgattgtc
     8581 ccatttttta gtacaatgtc agctttttct cttccacttg aaacatcaca ctgtcttttt
     8641 aaattaccca tttttacaac tcctcctaag ttttttatat caatatactg actgtattac
     8701 gtattatagt ttaatttatt aatatatatt atatatgagt tttgatagaa agtatcactt
     8761 gaaatttgtt ccataattcc tttaattaaa tagagagaac atgaggaata atattatctc
     8821 ccatgttctc attaatattt aagcaataat ttaattcata ttgaattttt tatttttata
     8881 tttaatcttc actattctta taaataaatt tatgtgcttt attatttttt taatacttct
     8941 ttattcataa ttatatttaa cctaaaaatg cataaactat catacctaca agtattgctg
     9001 gtatagtagt aataatcata gcccataaag aagatttttt atccattgct aaaagtggga
     9061 aaagtgcatc tccatcttga gaaatagagt tggctatcac tgcaccaagt ggtatagttc
     9121 ccttcaaata aaatgacata agcacaattt gtatcccaca acctggaata agtccaagac
     9181 ctgcacctat aaatacactt attacacctg ttgataatac taatttaact aagtattcat
     9241 ctccacctaa aattaatatt atcaaatcgt aaataagata tgcaataaaa atccatgtaa
     9301 ttacaaatga tatttctcca acagaatgga taagcatttc tcgcaaagaa atctttttat
     9361 tttcttcctc tttttgatta gaatttttaa ataccttttt aaaacaccac atataaatta
     9421 ttgaaaataa tattccaatc actgctatta cttcttccaa tgaatgtaac ttctcaatta
     9481 taggaagatt aagtccagag tgagcaagtg acataaatat aaaaccgatt actataattc
     9541 ctatataaat tttatatccc tttccatgtg ttattataaa tgcaagtcta tttgtatttc
     9601 catgagattt agctagagtg tctaaaatta tttcatcatg attatgttgt tctttatttt
     9661 ctttagggtt atttttactt atatcatatt tatctgattt tctacggttt tttaacccta
     9721 atttttcttc tagtttaaag taatccacta attgtccagt tattattcct gtaattgtac
     9781 taactatagt gacaaaaaaa tacattttaa tatttgatga aataagtaca aatgctgcat
     9841 ctcccatact agctattaaa cttgcaatta ttgctccaaa gcttaatttt ccatttatat
     9901 acaatggcat tattgccaaa cttccgccac aacctggtat agctccaatt aaagcaccta
     9961 ttaagggttg aaattttctg ttttttgata tgatattagt aaaatttcca ctagttttat
    10021 aattaatata tccaaataac aaaataaaaa atccaatcat agaacctaca tgtaaaaacg
    10081 cctcttcaga agcggatatc aacatttcca ttaatttacc tcctagacta attttgcaac
    10141 aattatatta tattctattt tctattgata atcaatatca atatttcttt tgaatttcta
    10201 atattaaaaa gtgcgcatct tatactctta gtttttcatc ttaagataca cactttttga
    10261 tattaggtta tctatttacc acatgtaata tgaaatcaaa ggtggatatt atgataaatt
    10321 ataacaatgt ccacctattt ttattaccta tcaaattata aattttcaag gaatgttaca
    10381 acttcttcta ttatccaatt aggtgtagat gcccctgctg taatacccaa atttttatat
    10441 tttttaagcg tatttacatc caattcatct ttagtctcta ttgcaaaagt tggtactaag
    10501 tcttcacaaa cctttactaa cttttgagta tttgaactgt gtttacctcc tataactatc
    10561 atacaatcaa cttctttagc taactcttta gctgattctt gtcttgtttt tgtagctgaa
    10621 caaattgtat cattaaatac tatttcttct aatttatctg atagtttatt aactatactt
    10681 atatacaact ctggatttat tgtagtctgt gcaacaagac aatatttttt ggagttatca
    10741 aattccatat tttccatttg ttctaaggtc tttattatta tagctgaatt ttcacaccat
    10801 ccatttatac ctataacttc tggatgcttc atatctccaa taactattat ttcatagcct
    10861 ttattatgat attcacttac tactgtatgt attttcttta caaatggaca tgtagtatcc
    10921 acaattttta attttttatc tttggcttcc ttatatatat tttcaggaac tccatgagac
    10981 cttattatca tattttcatg atttggtatt gtatcaattt cattcacagt cttaagacct
    11041 ttttcttcat attttgctac tgcttgcttg ttatgaataa gaggacctaa agcatatatg
    11101 ccagaatcat ttttttcaac ttcatcccat gccattttca ttgctctttt tactccaaaa
    11161 caaaagccag cattttttgc tatttttatg ttcataactt taccccctta ttttgaatat
    11221 ttaaagtaat tttgttttat cacttctaaa atctcttgtg atatcctttc atggtcatca
    11281 ttactcaatt tttccttaaa atgttcttca aatgatattg gttctccaat atatacaatt
    11341 actttactaa aaagtttata tttagatatt attgatactg gaacaacttt agatttactt
    11401 tttattgtaa atacagaaag acctgcctta gccttaccaa aagaatctcc ttttattcta
    11461 gtaccctctg gaaatattcc taatacataa ccatctctaa ctgaccttaa aatagttttt
    11521 attgtagata catctggttt ttctctattt ataggcataa catgcaattt ttttaatata
    11581 aatcccaagg gttttacttt aaacaattct ttctttgcaa ttgcagctat ttctctattt
    11641 tctatggcag cagccaagaa tatagggtca agattcgatt tatgatttga tgctattact
    11701 atatttcctc tatctggaat attttctgca cctataactt catatttaaa aaatatttta
    11761 gaaaaaccct taaaaatatt tattacaaat ctataaaaat tcacgtttat ctcccttcaa
    11821 cactacattt attttcatta tgatgcataa aaaactaaag atttatttta gatacaactt
    11881 cttctacaac ttcttctata gtttttcctg tagtatctat ttctagtgca tcattagctt
    11941 ttaccaatgg tgcaaattct ctatttgaat caatttcatc tcgtcttatt atatcctcaa
    12001 ttacttcttc taaagtagta ttatatcctt tattacaaag ttctttatag cgtctatttc
    12061 ctctttcttc tggagtagct actaaaaaga atttgtaatc tgcatctggg aaaacatatg
    12121 aacctatatc tctaccatct agtattactg aacttttcat tcctattttt cgttgaactt
    12181 caaccatcaa ctgtcttact tcttttattt ttgctacatt agaaacatta ttgcttactt
    12241 ctattgttct tatttcttca tttataactt cttcgtccaa atatatattg ttatctttaa
    12301 aatctatatc cgaattttca gctatttgaa taacttcttt tgcattattg acatcaatac
    12361 cactttttaa acacttataa gtaactgctc tgtacatagc acctgtatct atataattta
    12421 tatttagctt ttttgctact atcttggcta ttgtactttt tccagctcct gctggaccat
    12481 ctacagctat aactaaatta cccatatata cacctctatt tattcatatt taattattaa
    12541 aaaattatat ttatacactt atacccgcaa gatatccagt tgaaaatgct atttgtaaat
    12601 tatatccacc agtttctgca tctatatcaa ttacttcacc tgtaaaaaat aagttcttaa
    12661 ctaatttaga ttccatagta gaagaattaa tttctttaac tgaaatacca ccagaagtaa
    12721 ccataccagt gtttatacct gtagtcccat tacaagttag cttcatattt tttatatact
    12781 caataattct aatttcatct gccttagaca aatcacttgc ctttttatca actaaagata
    12841 atagagaaaa aatctctttt aagaaatttt gaggtagtat ttgcttaaga ttatttagaa
    12901 catttttatt tggaaatgct ctgattatac tagagatttc atcagtactt aaatttggta
    12961 agaaatctat atttaattct accttttcct tttcaataat cttgttaata taagaagaca
    13021 ttataagaac acaaggccct gttatcccaa aatgggcaaa tagcatgtct ccagattttg
    13081 atattttcct tttcttaatc ttacaagata tttccacatt cttcatggat attccttgta
    13141 aatctttaat ccatttttcc tctattgtaa gtggaactaa agcaggatat agtttgttta
    13201 atgtatgtcc atgcttttta agaatcttat acattgaacc atctgaaccc gtctttgaat
    13261 aactcactcc tccagttgat ataataacct tgtctcctag tatcttatct ccattatcta
    13321 aaataatccc ttttattaga tactttgact tgtccttatt agaatcatct tttagagcaa
    13381 tttcttcagt tataatatct ataactttct tattatacat tatctttaca ttgtgattga
    13441 gtaaatcttt atttaatact tctattactt ctatagattt atcattttta gtgtatactt
    13501 tgtggtcatt ttcttcttca atcttatatt ccaaatttct actttcaaaa tacgaaataa
    13561 ggtctttatt tgtaaatgta tagaaactac tatagagaaa ttttttatta ctgacaactt
    13621 tatcaaaaaa atcttctatc tctctattat tggtaaaatt gcatctaccg ccaccagttg
    13681 ccctaagttt tcttcctaat tctccatttc tctcaactaa tattacttca ttattgtttt
    13741 ttgaagctga caaagctgcc atcattccag aagctcctcc accaactact ataactttac
    13801 tcataaattt ctccttataa ttgattccta ctccagtgca gaattattta tctttacatg
    13861 attttttata tgacttgtat cattcatttt tactataaca ggtccataat ctttaaattc
    13921 tactatattt aaagcagtat tatcttgctt aattctatat atattttcca tacctgattc
    13981 taaaaatgac aacaacataa ccctcaatgt aattgaatgt gtaacaagaa ttatattttt
    14041 attatcatat ttttcattta gttcctttat aaaagcatct actctttcct ttataatctt
    14101 aagagtttcg ccttctggta tatttactaa gtgtggttca tttctccatg ttgcatatat
    14161 atctgaataa tctttttgaa tttcttttat taaaaggcct tcccaaactc cgaatcccat
    14221 ttctctaagt gcctcagttt tttgaacttc aatattgagc ttatctccta aaatttgagc
    14281 tgtttgcatt gctctcccaa ggtcgctact aaaaatataa tcaattgaat acttgccaat
    14341 atcttcagaa agttcttttg cctgttctat accttgaggc gtaaggtcag aattaccatg
    14401 accttgtgtt tttcccaata tattccaatc cgtttgtcca tgtcttacta tataaaaagt
    14461 atttcccata tgtcctccta atatatgcct attttttatt aaatataaat tatcacttct
    14521 caaaacttaa ttatatcata taatacttta ctttgtttct attttataat ctaatctaac
    14581 atattattta caataaatct catttgtatt tctgcaaatt attgtactgt taaagaattt
    14641 ttctaaattt ataaacatat ttctcttat
//
LOCUS       DAFKMX010000104        14308 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFKMX010000104 DAFKMX010000000
VERSION     DAFKMX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.104"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..316
                     /locus_tag="LZB08_002412"
     CDS             <1..316
                     /locus_tag="LZB08_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315908.1"
                     /translation="SINELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIY
                     ETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRYGEYYRFRI
                     KS"
     gene            331..915
                     /locus_tag="LZB08_002413"
     CDS             331..915
                     /locus_tag="LZB08_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315909.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1168..2205
                     /locus_tag="LZB08_002414"
     CDS             1168..2205
                     /locus_tag="LZB08_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBM7315910.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELVEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2286..2651
                     /locus_tag="LZB08_002415"
     CDS             2286..2651
                     /locus_tag="LZB08_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315911.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2656..2973
                     /locus_tag="LZB08_002416"
     CDS             2656..2973
                     /locus_tag="LZB08_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315912.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3021..4625
                     /locus_tag="LZB08_002417"
     CDS             3021..4625
                     /locus_tag="LZB08_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315913.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4749..6377
                     /locus_tag="LZB08_002418"
     CDS             4749..6377
                     /locus_tag="LZB08_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBM7315914.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6642..7991
                     /locus_tag="LZB08_002419"
     CDS             6642..7991
                     /locus_tag="LZB08_002419"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBM7315915.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8147..9226
                     /locus_tag="LZB08_002420"
     CDS             8147..9226
                     /locus_tag="LZB08_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBM7315916.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9571..10479
                     /locus_tag="LZB08_002421"
     CDS             9571..10479
                     /locus_tag="LZB08_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBM7315917.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKILFEASNFNTKIGG"
     gene            10484..12853
                     /locus_tag="LZB08_002422"
     CDS             10484..12853
                     /locus_tag="LZB08_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBM7315918.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRILQDEIIGRTEQTF"
     gene            13178..13981
                     /gene="proC"
                     /locus_tag="LZB08_002423"
     CDS             13178..13981
                     /gene="proC"
                     /locus_tag="LZB08_002423"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBM7315919.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5866 a         1456 c         2624 g         4362 t
ORIGIN      
        1 atctataaat gaacttgaag attatataag tcaaaataag ctagtggata aatcaaagaa
       61 cactttacca acaacagata aaactagaga gaaattttct aaaataaaac ttaatgaaat
      121 aaaaaaaata tatgaaacaa ttggaataaa tagtattact aaaatatcaa tagataaaaa
      181 taagctagag atggaaggtg tgtgtagtga tttagatgtt ttaaacaatt taaggaaaat
      241 aaagtacata aaatattatt acatagacag tattgtaaga tatggagaat attatagatt
      301 tagaataaaa agttagatgg gtgattattt ttgagattaa ataatagaga aaaaaatcta
      361 cttaaaatat tgttaatatt agttattata ttggcaagtg atatagcagt aagttatttg
      421 gaagaaaacc taactagttt gaaaaatgaa aagcaaaata taataaataa aataaataat
      481 caagataatt atcaaataaa ggataataaa catatagatg ataaatatat agaaagtgtt
      541 gaatcaaata aaaatgaaga attgaaaaat aattttaata actcattttt agataaaaaa
      601 acaaataaag atatagtgtt attagtagaa aatgaactga gagatattgt gaatgtagaa
      661 tatattaatc aagaaagtag tttagataat cacaataaaa aacaagttaa tttagaaata
      721 aaagttaatg gagaacttgc aaatgtgttg aaacttgaag gtgtaatgaa gaagttaaga
      781 ctaaataata aacttgaaaa gatagaaatt aataaattaa aagtcaataa ttttgatgtt
      841 caaaatattc caaatgaata taaacaagaa aaagacaata ttgttgaatg taaaatgaca
      901 gttagagtca tataacaaaa aagaatagat aaagtaaaaa actattatca attaataaaa
      961 ttttatagag gaattagttt tacgataatt catttctaca agagggattg ttgatgattt
     1021 tatcgaaatc ctaattaaag ttaatatatt gtaccaaaat ataagtgaat ttaatataat
     1081 atttacttat actatagatt gattaataaa aaaatggtat aataacaaat tgtactttaa
     1141 tttaataata agggagtgga agtcatattg ttagggagaa atgagctatg cccttgtgga
     1201 agtggcaaaa aatacaagaa atgttgttta aataaagata ttgtttcaga aagagcatgc
     1261 agaaaaactg aactttcaca aaaacaatat acagatttat attcaaagtt atatgattat
     1321 tcaagacagg ataagtttaa agaagaatgt gaaaaagcta aagaaatgtt ttatataatg
     1381 caaaatgata gtgtaaatga aaaatttgaa agatttttta atacttattt aatacaagac
     1441 cacataatgg aaaataaaaa ggttatgaca gttggttttc ttgaagaaaa tggtgcaaac
     1501 ctaagtcaat cagaagtatc aatattaaaa agtatgtttg aatcatacgt aagtatttat
     1561 gaggtaaaag aagtttctgc tggaaaaata gtattaaagg actgtttaag tggaaatgaa
     1621 ctttgtacag aagatgtaaa acttctgaga agttttaaaa tcggaagttg tatgatagct
     1681 agaatagttg atattgaagg aactaacata ttgatagata taactataag tatatcaaat
     1741 gaggttaaag atataatttt aaaggatatt atgaatctat tcaatcaata tcaagatgta
     1801 tacaaagata tgaagtcgtt cttaatttat catacacata tactatacaa gtatatacaa
     1861 cagcttttag acccaagtat agctgaatat ttaaagagag aaagagataa aaacgataca
     1921 aaagttaatt cagaaaatga attagttgaa gaagaatgta aagttttaga tactcttaaa
     1981 gaaaatgtag aaaaagaaaa tatagatgca tgtatagagt tttggaatga atataagaaa
     2041 aatcatgaaa atataaaagg agctgaaaat ggttgggcag ctgctgttga atactatgtt
     2101 aaaaaagaag caggacaacc tataactcaa gttcaaatat caaagaaata cgatataagc
     2161 tctagtacac taggaaagag atacaaggat ttaaaaatat cttaattaat tataaaaagc
     2221 tttaagtcat agataaagaa tctatagtat aatatatgta taatgtttag aaagaggtat
     2281 taataatgag tcttagttta ggaaatgcaa ttgctgtatc aacgaacttt ataaaatcta
     2341 acccagctgt acctgtgtac aacggagaag aaatatatgc agttatttat catatagaaa
     2401 taggatataa taatggtgta tcacctgata ccaaacaata tataataata gactataaag
     2461 agtttgattt atctatggat aaaaaaaatg aactaataaa tattgctaaa gagtattgta
     2521 aatctaaagg tattgtagaa aacgaaaacc ttgaaataaa ccttttagac aatgaagaag
     2581 cagagaactt cttagaaaag gtgtttaata atatgaaggt tataagaggt cttataacta
     2641 aaaaaggata aataaatgga ctatatagga atagaaaata taacacctta tgaaaataca
     2701 tatgaattta gtgtatatga atatgatgat gaaatcacct taggtagtga aaagttatat
     2761 gtatgtgaat taagggttgt attgattaaa gttaattctc tgtatgttga aagattgcat
     2821 aaatcagttg aagcaatggt cttagtaaaa aacttgaaaa aagatttaga taaaacactt
     2881 gttgtaaaca aaataaagaa ttttgtgcta gatgagattt gggtagaaaa tctagtaaaa
     2941 gagaatatag aagttatatt tgtagaaagc taggaataac cctagctttt ttatattaca
     3001 atctaagaga ggaaaacaat atgttaagag tatctaatgt aaaacttgga attgataaag
     3061 atatatcttt attaaaagat gttatactta agaagctaaa aataaaagaa aaaaacttag
     3121 tcaaatatag tatatataaa gaatctatag atgctagaaa aaaaggaaaa atggaatttg
     3181 tttattctgt agatgtagaa gttaaagatg aaaataaaat acttaactca aaaataaaag
     3241 atgtaacaaa aataaaggaa atcagatacg taaatgtacc tgtgggtgat aaaaaattaa
     3301 agaataatcc tttggtaatt ggtagtggac cagctggttt gtttgcaggt ctgcttttag
     3361 ctcaaatggg ttatgaacca atcatacttg aaagaggtct tgatgtagac agtagaacca
     3421 aagatataag tgatttttgg acaactagaa aattaaaaaa taattcaaac gttcaatttg
     3481 gagaaggtgg agcaggaact ttttcggatg gtaaacttac aactagaata aaagatatac
     3541 gatgtagaaa agtattagaa gagttagttc agtttggagc accagatgaa atattatact
     3601 cacataagcc acatgttgga acagatatat taaaaaatgt agttaaaaat ataagaaatg
     3661 aaataataag tttaggtggt caagttagat ttaattcaaa agtaacagat atagttgtat
     3721 caaatgattc tatacaatct gtaataataa atgatactga aaaaatagat acagatacaa
     3781 ttatattagc tgttggacat agtgcaagag atacttatga aatgttgtat tcaagaggta
     3841 taaaaatagt tcaaaaacct tttgcaatag gagctagagt tgaacatcca caagatttaa
     3901 taaataaagc acagtacaag gatttttata atcatcctag gttaggagct gcagattata
     3961 gacttataga gcatgtttca aatggaagaa cagcatatac tttttgtatg tgtccaggag
     4021 gtagtgtcat agcatctgca tcaaatgatt ttgaaatagt tacaaatggt atgagtgaac
     4081 atgcaagaga taaaataaat gcaaatagtg catttttagt aaatgtaacg ccagaagatt
     4141 ttaagagtga taatccacta gcaggtgttc atttccagca gaagtatgaa aaattagctt
     4201 ttgaattagg tggaaaaaat tataatgcac cagttcagct tgttggagat tttataaaca
     4261 ataagacttc tttgaattta ggaagtgttg agccatcata tagaccagga tatacactta
     4321 caaatttaag tgaatgtttg cctagcttcg taacagagac tatgagagaa gcgtttatat
     4381 ctcttgataa aaaattgaat gggtttgcaa tgtatgatgc tgttttgacg ggagtagaaa
     4441 ctaggtcatc atcaccaata agaatagtaa gagatgaaaa tactttagaa tcagtgagta
     4501 ttaagaatct atatccatgt ggtgaaggtg ctggatatgc aggtggtata gttacagcag
     4561 cagttgatgg tataaaatgt gcagaaaaaa taatacaaaa atacaccaat aatacaaaaa
     4621 tttaacataa acttaatgat tttttaatat ttaacatgaa tttaacatta tcactcctat
     4681 aatttgataa aatacaatag gttataaaat ttttagatac agtaatctct ttacaaggag
     4741 gaaataattt gaacattata ataagtctaa tgggtggatt aggtctgttc ttatatggta
     4801 tgaaccttat gggagaaggt cttcaaaaat ctgctgggac aaagctaaaa aagataataa
     4861 aactacttac tagtaaccta tttatgggag ttttggtagg tacaggagtt acagcagtaa
     4921 tacaaagttc aagtgctact actgtaatgg ttgttggttt cgtaaatgca ggaataatga
     4981 ctttaaaaca ggcaatagga gttataatgg gtgctaatat agggactact gttacagccc
     5041 aattggtatc tttcgactta actggtatgg ccccagtagc attaggtgta ggtattatac
     5101 tttatttatt tggaaataag cctagaataa aaaatatagc cgaaatactt ataggttttg
     5161 gtatattatt tacaggtatg gattttatga agatggcagt ggagccacta agagactatc
     5221 aaggatttac tgatttactt gttacatttg gtagatatcc actacttgga ttattattgg
     5281 gatttgggat tacagcaata atacaaagtt caagtgcatc tatgggtatg ttggttgcac
     5341 tggcagcaga aggtctagtg ccattatcag cagcactgcc aattctttat ggccaaaata
     5401 ttggtacatg tgtaacttca ctgttatcaa gtattggtgc taacaaaaat gcacgaagag
     5461 cagctatgat gcatctaatc ttcaatgttt taggaacagt gatattctta atattcttga
     5521 ataaaccagt agtaagcatg gttacaagtt gggacccaag caatgttgca agacaaatag
     5581 ctaatacaca tacattgttt aatataatat ctgtattgat acttttacca tttacaaatc
     5641 ttattataaa actagctata aagcttgttc ctgatagagc aggtgatata gatgaagatg
     5701 aaactaaaac tattaaatat atagatgata gaatgataga aactccttct atagctcttg
     5761 caaatacgat aaaagaagca cttcgtatgg gtgagaaggc gaaggaaagc ctaaatgcat
     5821 ctatggaagc tttagtagaa cattcaactg aaaaaataga taagacatat aggcgagaga
     5881 gattaatcaa tgatttacag aaagctattt taaattattt acttaaactt tcaaaagcac
     5941 ctttagatga tgattcaaga gaagttgtgg atactttgtt taatacagtc aatgatatag
     6001 aaagaattgg tgaccacgct aaaaatatag ctgaattatc gcaagttgca atagattcaa
     6061 atatttcttt ctcagaagaa ggacaatctg aacttgatgt tatgtataat agagttgttt
     6121 cagcatatac ttatgcatta gaatcaatga gaactgataa tgttgattta gcttgtaagg
     6181 ttataaaaat tgaagaacaa gttgatataa tggagaaatc ttgtagagct aatcacatgt
     6241 atagattgaa caataatcta tgtagtatag aaaatggagt tatttattta gacgtaataa
     6301 gtaatttaga gagaatatct gaccatgctg tcaatatagc acaacaagtt atagcaaaga
     6361 gattaggaaa tgattaaaaa taattaattt aaaataatat aaaatttaaa atagaggata
     6421 aataaaagag aaactttaaa tgatacgttt agagtttctt tttttgtaaa tttatttgac
     6481 taaaattttt aaatttatac atagttatag catttagaag aatcgatttc ctgataataa
     6541 atgatgagat atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat attttgtgtt
     6601 ataatctctt taaaacgaat atgctgaaaa gagggattta aatgggaaaa ataaattttg
     6661 attatagtaa agctacagag tttttttgtc aaaacgaaat agatgtcatg caaccatatg
     6721 tagatgttgc gcacgacatg ttacataata aaactggttt aggaaatact tttttaggat
     6781 ggatagacct tcctaaaaat tatgataaag aagagtttga cagaataaaa aaatctgctg
     6841 agaagataaa atcagactca gatgttcttt tggtaatagg tataggaggc tcttatttag
     6901 gttcgagagc tgctatagat atggtaagtc attcttttag gaatggactt aaaaaagagc
     6961 aaagaaaggc tccagaagta tattttgtag ggcataatat aagctcaaca tatattatgg
     7021 atttattaga tataattgaa ggaaaggata tttctgtaaa tgtaatatct aaatcaggaa
     7081 ctactacaga accagctctg gcttttagaa tatttaaaga ttacttagag aaaaagtatg
     7141 gtaaggaaga agcaagaaag agaatttatg caacaacaga tgcaagtaaa ggtgcactta
     7201 gacagttagc tacagaagaa ggatatgaga cttttgtaat accagatgat gtaggtggtc
     7261 gtttttctgt attgacagca gttgggttac ttcctattgc tgctgctgga cttgatattg
     7321 atgctatgat gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct gacttaaaaa
     7381 gtaatgatag ttatagatat gctgttgcta gaacaatttt acatagaaaa ggaaaagatg
     7441 tagagttatt agtgaactat gaacctcaac tacattatgt aagtgagtgg tggaaacagc
     7501 tttatggaga aagtgaagga aaagaaaata aaggtttatt ccctgcttct gtagatttct
     7561 caacagattt acattctatg ggtcaataca tacaagatgg aaaaaggctt ttatttgaga
     7621 cagtacttaa tgttgaaaac tgtaagagaa atataactat aagttcagag gaagtagact
     7681 tagatggttt aaactattta gcaggtaaga ctgtagattt tgtaaatcat aaagcttttg
     7741 aaggaactct attggctcat acagatggaa aagtacctaa tttagtaata aatatacctc
     7801 aactagatga gtataatttt ggatatttag tttatttctt tgagaaggct tgtggaataa
     7861 gtggatattt attgggagtt aatccatttg accaaccagg agtagaagct tacaagaaaa
     7921 atatgtttgc tttactaggc aaaccaggtt atgaaaaaga aaaggaagag ctagaaaaaa
     7981 gacttaaata attttaaata gttaaggttt aattaagatt aaaataatat aatgaaatca
     8041 tcaaaaagga gtttactcaa aatagatgat ttcatttttt ttatgaaaaa ttgggaaaaa
     8101 tatattttga taatttatat tatattaaga tagggagtgg tatgagatgt caagaagaaa
     8161 gaaagggata agtttagtaa tattagttgc tataataagt tcaatactta gtagttttct
     8221 aacaataatt ttagttaagg acaatttagt aagtaaatct acaggaagtt ctacgccgat
     8281 agttgtaaat gatgatggta agagtcaaaa tatttatcaa gcagttgcag aaaaagctac
     8341 gccatctgtt gtaggtatta caacaactag tgttgatact agtaatatgt ttgcaatacc
     8401 aactgaaaca caaggtgtag gaactggaat catagttgat tcaaatggat atatacttac
     8461 taattctcat gttatatcag atggtcaagc tacaagtgta aatgtattat tcaatgatgg
     8521 ttcaacaact tcaggtaaag tagtatggtt tgaccaacaa cttgatttag ctatagttaa
     8581 ggttgataaa actggactaa caccagcaga atttgctgat agtgacaaag taaaagttgg
     8641 agatatatca atagcaatag gtaatcctct tggattagat tttcaaaaaa cagttactca
     8701 agggataata agtggattag atagaactat acaaacagaa aaaactaata tgactggttt
     8761 attacaaact gatgcaagta taaatgcagg aaatagtgga ggaccattgt taaatcaaaa
     8821 aggtcaagta attggtataa acacagcaaa agcttctcaa gcagaaggat taggatttgc
     8881 aataccaatc aatacagcta agagtatagt tgaagaagta atcaaaaatg gaaaatatga
     8941 aaaagtaact ttaggcataa aaggtacaga tgtatcaaat tatgaagcag caacaggtac
     9001 aaaattaagt actgataaag gtgtttatgt tgcagaagta atatctggtt catcagcaga
     9061 aaaagctgga gtaaaggtag gggatattat aactaaagta ggagatacag atataacagg
     9121 tatgaatgat ttaaataaaa agttgtatac tttctcaaaa ggtgctagta caaaaataac
     9181 agtaaataga ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat gaccaaaaag
     9241 atagagacta catggtaaag tgtagtctct atttttattt aatttttata gtatatacat
     9301 ctaaattaat taaaatgatg tattgtttat agtttattgc tatattttct tataaaaata
     9361 aatttaataa atatttaaat ttaatttaca aaaacaatat aaatatcata ataaaataaa
     9421 attttttaat attactaaaa atatatagac aaaataataa caaatatagt attatgtaat
     9481 tgtagaaatg cttttcatag gtatattcta tgaagatgaa ttgcataaaa atgaaaaaat
     9541 ggaatggata ttatttaggg gtgaaaaatc atgaatccat tagttataaa cttacaaaaa
     9601 tgtagcatac atgatggacc agggataaga agtacagtat tttttaaagg gtgtccactt
     9661 gaatgtgttt ggtgtcataa tccagagagt caaacatata caaaacaagt tttgtataat
     9721 gaagaaagat gttcaaaatg tgaagcatgt ataaatatat gccctcataa ggcaatttac
     9781 aaaggtgaaa caaaaatatg cttagaccaa gataagtgtg agttttgtga aacttgctta
     9841 gactattgtg taaacaatgc aagagagatt gttggtcaag agtattctgt aagagatttg
     9901 gttaaagaga tagagaagga tagaattttc tatgaagaat caggtggtgg agtaactcta
     9961 tcaggtggag aagtaatggc tcaagatatg gacttcattt gtggagtaat aaacatgtgc
    10021 aaatcaaagg gaatccatgt agcaattgat acatgtggct atgcaaagtc agaaaattat
    10081 gaaagagttg ctaaatgtgc agatttgttt ttatatgata ttaagcttat tgatgaagat
    10141 aagcacataa aatttacagg aaaatcaaat gacttaatac tgaaaaatgt taagatttta
    10201 agtgaattag gggctaacat aaatataaga ataccattaa ttgtaggagt aaatgtagat
    10261 gatgaaaatt tagaggttaa aaaaatgata gagtttttaa agcctttgaa tatacaagca
    10321 gtaagtcttc ttccgtatca taatattggt aaacacaagt atgataaaat ttataaaaag
    10381 tatgaaggag aagaactaca aagaccatca gaagaaaaat tagaggaaat aaaaatactg
    10441 tttgaagcaa gtaatttcaa tactaagatt ggagggtaat aaaatggcaa gaggaacttt
    10501 tgagagaact aaaaaattaa gagaagaaag tattaatgca gagccacaca tatctattga
    10561 aagagcagta cttatgacag aagcttataa aaaatatgag ggaagtgtag agattcctgt
    10621 acttcgtgct ttatcattta agcattatat tgagaataga actcttagca taaatgatgg
    10681 agaattaata gtaggtgaaa aaggtgattc tcctaatgga gcaccaacat atccagaaat
    10741 ttgttgtcat acaatggaag atttagaagt aatgcataat agagatataa taaattttag
    10801 cgtatcagaa gaagctagaa aaatacataa agaagaaata attccttttt ggaaaaagag
    10861 acaaacaaga gataagataa taaatgcaat gacacctgag tggttagcag catatgaagc
    10921 aggtatgttt acagagttta tggaacaaag agccccagga catacagttt gtggagatac
    10981 aatatataaa aaaggatttt tagatttaaa gaaagacata gaagcaagat taaaagagct
    11041 agattttctt aatgatttag atgcatataa taaaaaagca gatttagaag ctatggcaat
    11101 agcttgtgat gcaatggtta tattaggtaa aagatatgct gagaaagcaa gacaaatggc
    11161 agaagaagaa acagatgaag ctaagaaaca agatttatta ctaattgctg aaacttgtga
    11221 tgttgtacca gctcacaaac cagaaactta tcatcaagct atacaaatgt attggtttgt
    11281 tcatataggg gttactactg aacttaatat atgggatgct tttactccag gaagacttga
    11341 ccaacatcta aatcctttct atgaaagaga tgtagaaaat ggaatactag atagagatag
    11401 agcacaagaa ttacttgaat gtttatgggt taaatttaac aatcagccag caccaccaaa
    11461 agttggtata acattaaaag aaagtagcac atatacagat tttgcaaata taaacactgg
    11521 tggaataaat ccagatggtc aagatggtgt taatgaagtt agttatataa tacttgatgt
    11581 tatggatgaa atgaaattga tacaacctag ttcaaatgtt caaataagta aaaagactcc
    11641 tcaaaaattc ttaaagagag cttgtgaaat atcaagaaaa ggatggggtc aaccagcatt
    11701 ttataatact gaagcaatag ttcaagagct tatggaagct ggaaaaacta tagaagatgc
    11761 aagacttggt ggaactagtg gttgtgttga aactggatgt tttggtaaag aagcatatgt
    11821 tctaactgga tatatgaaca ttccaaaaat acttgaatta acattaaata atggatatga
    11881 cccaattagt aaaaaacaaa taggtattga aactggagac cctagaaatt tcaaatctta
    11941 tgaggaacta tttgaagcat ttaaaaagca attacattat atgattgaca taaaaataga
    12001 aggaaatgct gtgatagaaa atatatgtgc aaaacacatg ccttgtccac ttatgtctac
    12061 aatagttgat gattgtatag aaaaaggcaa ggattaccaa agaggtggag caagatacaa
    12121 cactagatat atacaaggtg ttggtatagg aactataaca gatagtttaa cagcaataaa
    12181 atataatgta tttgataaga aaaaattcga tatggataca ttgcttaaag cacttgatgc
    12241 taattttgaa ggatatgaag ctatacttaa tttagtttca aataagactc ctaaatatgg
    12301 aaatgatgat gattatgctg atgaaataat gcaagagata ttcaatgcat attacaatga
    12361 ggtaactggt agaccaactg tttgtggtgg agaatacaga gtagatatgt taccaacaac
    12421 ttgtcatata tactttggag aaattatggg agcaagtcca aatggtagac tttgtgcaaa
    12481 accagtttca gagggaattt cacctgaaaa aggtggagat actaatggac ctacagcagt
    12541 tataaaatct tgtgctaaga tggaccacat aaaaactggt ggaacattat taaatcaaag
    12601 atttgcacca tcagtagttc aaggagaaaa agggttagat aatatggcta atctagtaag
    12661 agcatacttt aatatggatg gtcatcatat acaatttaat gtatttgata aaaatgtact
    12721 gttagaggct caaaagaatc ctcaggatta taaagactta atagttagag ttgcaggata
    12781 tagtgaccat ttcaataatt taagtagaat actacaagat gagataatag gaagaactga
    12841 gcaaacattc taggtttgct tgtaaataaa acttcttact atataaaatc gtaactgata
    12901 taataaagta taaatggttt gattccattt ttaaaatttt agattaatta tgagctaact
    12961 ttctaaatag gaagttagct cgttaagttt aatgaaaaaa tcgttaaatt tatatttaat
    13021 aaatcataca catattagta agtaatacat acaaaagaat aatttattat agttataata
    13081 aggttatatt ttataaaaat aagataattt taagcagtta aaataaagtc aatttagcta
    13141 ctttaaaata aaactaatag aaaaaaggag atttgatatg aaaactttag gatttattgg
    13201 ttcagggaat atgggaagtg caatgatagg tggcatagta cagtcaggat tagtaaaggc
    13261 agaagatata actgtatcag atttaagcca agttgcctta gataaagtaa aagaagaatt
    13321 tggagtaaat actacaactg attcaaaggt agctgttaca aattctgatg tggtaatagt
    13381 agcacttaaa cctaatatat gcgaaaaggc tttaactcca cttaaagaat taatagatgc
    13441 aaataaaata atagtatcta tagcagcagg taaaactata gaaagtctgg aaagttttat
    13501 aggttcagat aaaaaaatag taagagtaat gcctaatact ccagcccttg ttggcgaagg
    13561 aatgtcagct ctttgtaaaa atagtaatgt aacagatgaa gagttaaata tgattaaggc
    13621 tctgtttgaa tcttttggtg aggcagaaat agtatcagaa tatttaatgg atacagttac
    13681 aggtattagt ggttcttctc ctgcatatgt atttatgttt atagaagcta tggcagatgc
    13741 agcagtatta gctggaatgc caagacaaca agcttataaa tttgcatctc aagcagtaat
    13801 ggggtcagct aagatggttc ttgaaactgg aaaacatcca ggtgaattaa aagatatggt
    13861 ttgttctcca gcgggaacta ctatagaagc tgttaaggta ttagaagaag aaggatttag
    13921 agcagcagtt ataaaatcta ttgttgcttg tatagataag tctaaagata tgagtaaata
    13981 acagatgtaa ttaagtttac tatatggttt cttgaattgt ttttttagtg cttatttata
    14041 tctataaata aatgtatttg gtataagtaa aataagagta atttactata aaaaggaatt
    14101 taatgattga gtgagaattt attacttaaa tattaataat taaatagata tatttttaac
    14161 tatatttgag atatatcatt gtgaaaaaat aaaatttata agtattattg tgaggttata
    14221 agtagattta tttaaaaata aatttattta tagccttttt aaatttgaaa taatattcat
    14281 aaggtaataa aagttacaaa tattttct
//
LOCUS       DAFKMX010000105        14266 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFKMX010000105 DAFKMX010000000
VERSION     DAFKMX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.105"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            131..844
                     /locus_tag="LZB08_002424"
     CDS             131..844
                     /locus_tag="LZB08_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBM7315920.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            962..1831
                     /locus_tag="LZB08_002425"
     CDS             962..1831
                     /locus_tag="LZB08_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBM7315921.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(1986..3131)
                     /locus_tag="LZB08_002426"
     CDS             complement(1986..3131)
                     /locus_tag="LZB08_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBM7315922.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3464..4240)
                     /locus_tag="LZB08_002427"
     CDS             complement(3464..4240)
                     /locus_tag="LZB08_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBM7315923.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            4822..5796
                     /locus_tag="LZB08_002428"
     CDS             4822..5796
                     /locus_tag="LZB08_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBM7315924.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            5894..6199
                     /locus_tag="LZB08_002429"
     CDS             5894..6199
                     /locus_tag="LZB08_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBM7315925.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            6354..7514
                     /locus_tag="LZB08_002430"
     CDS             6354..7514
                     /locus_tag="LZB08_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBM7315926.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            7564..8466
                     /locus_tag="LZB08_002431"
     CDS             7564..8466
                     /locus_tag="LZB08_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBM7315927.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            8546..9439
                     /locus_tag="LZB08_002432"
     CDS             8546..9439
                     /locus_tag="LZB08_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBM7315928.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      9492..9673
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9901..11031
                     /locus_tag="LZB08_002433"
     CDS             9901..11031
                     /locus_tag="LZB08_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7315929.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            11103..12128
                     /locus_tag="LZB08_002434"
     CDS             11103..12128
                     /locus_tag="LZB08_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBM7315930.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     TIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            12131..13327
                     /locus_tag="LZB08_002435"
     CDS             12131..13327
                     /locus_tag="LZB08_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315931.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(13455..14180)
                     /locus_tag="LZB08_002436"
     CDS             complement(13455..14180)
                     /locus_tag="LZB08_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBM7315932.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIIAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         5382 a         1641 c         2487 g         4756 t
ORIGIN      
        1 ttataattaa ttataaatat tgaataatct aaatggataa gataatggta ttaaaaatta
       61 tcttaaaagg tgaaataatc tgtatttaat gtagtacagt tatatcttta aagcaaggtg
      121 gtgaaatgat ataaataaga tatctaaaag agggtttgat gatagtgata aaagatggtt
      181 ttcaagtaaa gaattaacaa gacttataaa ggctaaagaa gaaatagaat ggttattaaa
      241 tagggattac aaaatagacc cagtcgttac ttttgttggt gatagatatc agttttctat
      301 aagacaaaga gatgcattaa aaagagcagt ctgtactgat gaaaaaaata ttataagaca
      361 atcaaagagg ctttctctag ataaaatcaa agaagatacc gtaaatatag atggctttaa
      421 tttgataata tccatagagg taggattatc tggtggtact ctagtaatag gaagtgatgg
      481 caatataaga gatttagcag gtcttagggg aacctataag attatagata aaacagaaga
      541 agctttgaat ctgataggta aattttttaa taaatacaat gcacagaatc ttaaatttta
      601 tttagatgct ccagtttcta attcaggaaa tttaaagtat aggatattag agcatgccaa
      661 aacttgggga attgaaactg aagtagaatt agtaaaaaat gcagatgttg tattggaaaa
      721 attagataga gtagttagta gtgatgcagt catagttgac aagtgtataa gttattttaa
      781 tgtggctaga ggtataattg aagagtatat taaagaatgt aatatcataa atttaaataa
      841 atagataaat ataggtgaga atatataagt atatgtagca atattcttag taatacaagt
      901 aaagtttgaa aataggagaa cgaatacata tcgaatttca taaaatgttg taaaaagaca
      961 tatgagagga tggatatgtg tgaaattaac taaactaaat attaaaaaat atagagcacc
     1021 tatatataaa tatgctttag caaatataaa tttgaggtct gctaaatcta ccaattcaag
     1081 tatcataaca gtaatacctc agggagccaa aatggaagta ttggatgagg aagatgactg
     1141 gattaaggtg atgtacaatt ctcaagaagg atatgtgtat aaggagttgg tatcagtaag
     1201 cgaatatgct tggagtaact taaacttaag agaagataaa tctacaactt ccaatataat
     1261 tacagtaatt cctgaaaaat ctagggttga agtccttcaa gtagatggag attggagtaa
     1321 ggttgtatat gatgataaaa taggatatgt ttttaattat tttttatcaa ttgatgggaa
     1381 taaaccaaat gaattagatt ataagtattt ttatactgac atgattaaat ttgttaacga
     1441 aaataatatt aaaagtacta gcgattatct aattgtaaca gaccttagaa ataaatatac
     1501 ttatattttt aagaaagata atggaggatg gggacaactt tacaagtggc aatgtactat
     1561 aggcaagcct gagactccaa ctataacagg tatattttat ataagtggaa gaaaaccttc
     1621 ttttggaacg gatgagtatt cagttaagta tgctactaga attaaaggtg gatattacta
     1681 tcattctgta ctatatgatt caacaggaag ttatataata gatggcagac ttggtgaagc
     1741 tcttagtcat ggatgtataa gattaagtac ggaaaatgca aagtggatat atgataatat
     1801 accagataca acaacagtta taattcatta agaaaaattt tgagtttttt attgggataa
     1861 tgattcttaa attaatagga ttatatatta acttctcata aagagtacta tttaaaaagt
     1921 gtaataagag agtgctatta acaaaacaaa gatattttga ggcactctct tataaatata
     1981 cataattaag atattttcat aaacaattct ataatcttat ctttatcaaa gaaaacagga
     2041 ttagcaccag cacaagcatc tttcatagag ttttctgcaa gcaattcaaa attaggatta
     2101 tcaacaccta catcttttaa agattttggg attccaacct cttcagaaag agcttttatc
     2161 ttatctatta caaagtcaac acactcttta tctgatttat tatttacatc cattccaata
     2221 acttcagcta ttggtcggaa tttggcaggg gctgatttag cattttcctc ctcaactatt
     2281 ggaagtagca tggcattaca tactccatga ggtaaatcat ataaacctcc taattgatga
     2341 gccatagcat gtacatttcc aagacctgca ttactaaagg caattccatt taaaaaacaa
     2401 gcataacaca tttgttctct agcttctatg tcattaccat ttttaacagc tcttggaagg
     2461 tatttaaata tctgctttat agcatataag gcagttgaat ctgtaacatc ataagcacca
     2521 ttagctacta cagcttctat agcatgagtt agtgcatcca taccagttgc tgctgttaaa
     2581 ttagatggtt tgctaatcat taattctggg tcatttactg tcattgtggc tagtgaattg
     2641 gtatcaacca ttatcatctt tacatgtctg tcttcatcag ttataacata gtttattgta
     2701 acttcagcag atgtaccagc agtagtagtt atagctatga taggaagaca ttttttagaa
     2761 gttttgttga taccttcata gtctctaata tctcctccat tagtggctaa tatagagata
     2821 gctttaccac agtcttgtgg agaaccacct cctatagtta taacgaagtc gcaattttct
     2881 gattttaata ttttaagtcc tttattcaca ttagttacag ttggattttg tttaacttca
     2941 tcataaacaa caaagtctat atctatctga gataaaatat ctgttacttt ttttacagta
     3001 ccatttgaaa ctagaaattt atcactaaca ataaatgctt ttttagttcc taatgattta
     3061 attgaatctt tagcttcatt aagacatcct ttcccaagta aatttattgg tggtacataa
     3121 taaatgctca taactaaaat ctccttttat tttcattgat ttttatgtaa aaatatatat
     3181 ctaacaattt tcactaattt actcataata tatcgtatct tagattgaga tagggttgct
     3241 ggttcttata taagaactta attcgtaatt acgattatga ttagaattta acatagtatt
     3301 tttatcatgt caataaaaat ataaaaaata ttataaaaaa aacaaagtta ctgtagttaa
     3361 atcagcaact ttgtttttat agtataaatc aaataaatac attagtttaa aacaaaattt
     3421 ttaattatta tgttttgata tcttccatat acagaatatt aatctaattt attacaatgt
     3481 cttcattacg ttcttccatc attgattgaa tatttttctg agcatttaag agtaattttt
     3541 ttatcagttc tattttttcg tcattagctc taaataaagg cagtgttaca ctaacagcag
     3601 ctcttataca tccatttttt cttattggta cggcaagaca ttttaggtgt tccattgatt
     3661 cctcacactc tgaagctatt tgtgttcttt gaacttcaag aagctggtcg tataaaacat
     3721 caaaatctgt aattgatttt tctgtaaaac cttttagacc atctggatat aagcttatta
     3781 gtttttcctt gtcaaaatca cataaaagag cttttccaag acctgtacaa ttagcaggta
     3841 ttttttttcc aacataagaa ataagtctaa tcggttctgg agaatctact tttccaatat
     3901 aaagaacttc attattgtca agtataccaa gctgacaagt ttcagaacag gaattaacta
     3961 tattagtcat ttcgtcaatt atatagttaa aaatagttct tttttcaaaa aatgaagacc
     4021 ctaatgtgta agaacgtatc cctatagaat atttggatgt gtttttatca atagtaatgt
     4081 attttctagc attaagtgta tgaactatag gaaagatgct actttttgga gcattgatag
     4141 catctgctat ttctgtaaga gtatatccat tttcggattc tgctaatgct tctaaaatat
     4201 ctaatatcct agctgttggg cgatgattat ctgaagtcat aaatttcccc ctctttttat
     4261 taatttaaag tattagaata tgttgatttt agcacactat acgatatttt acaatgtaga
     4321 aaataaatat gtaaatttat atataaataa gctcttgatt tagatacaga tagatatttt
     4381 tttagactgt acattaagga ataactaatt tccaaaatat acctaaagaa agtaagtgat
     4441 atttagttat ataatttaag taaatatatg caatagttat ttttatatgt aaatatcaaa
     4501 atattatgaa cttatatttt atctattttt tacactgtat aaaaacttta tagttttgaa
     4561 tactatataa ttttaaatac gctgtatttt tgcatataag gaagtcattt gataatatac
     4621 aaggtattat cagaaagttt gaaaaaattt tgacaaaaat ttattttttt tattgactca
     4681 taaataggaa aatgctatca ttaattcaaa gattcgtaat taaaaactta gttcttaaat
     4741 acgaaccgaa acggccgtta gaattaaagc taaaaactta ataagagtaa ttaaatatca
     4801 tgttttagga ggattaactt tatgataatg gattttatta aaaaaattcc tgcaggtatg
     4861 atgatagtgc caatgtttat agcagctttc attaatacct tttgcccaga tattgtacaa
     4921 ataggttcgt ttacgacagc agtattttca agtgttgggt ctgcaactat aattggggtt
     4981 caactggtat gcttgggaac tcagcttcaa tttagagaaa tgccaaaagt tttaaagaga
     5041 ggtggtatac ttcttggttc taaatttgct ataggtgctt tgataggtat acttatagga
     5101 aagctatttg gaatggatgg tgtccttggt cttacaacat tggctgttat aagtgcagtt
     5161 acaaatagta atggtagtat atatctttca ttgatgactt catatggaga tgaaacagat
     5221 tgtgcaacta tgagtttact tactttaaat gatggaccat tttttacatt aatagctctt
     5281 gggacttcag gtcttgcaaa tgtaccattg ttgtcattat tggctgcaat agtgccaata
     5341 cttgttggta tgataatagg aaattttgac aaaaaaatga aagagttttt agagccaggg
     5401 tgtaatttac tcgtaccttt tgtagggttt gcacttggta gtagcataaa tcttgcaagt
     5461 atactaaaag gaggtttatc aggagtatta cttggattaa tatctgtatt tgtaggaggt
     5521 atatttatag tattctgtga taaatttata ggaaaacgtc caggatatgc aggatgggct
     5581 gtagctacaa ctgctggaaa tgcaattgct gtacctgctg ctgttgcatt agttgaccca
     5641 tcatggcaac catatgttgc aacagctaca acacaagtgg cagccgcagt agttgtcact
     5701 gcaatattag tacctcttat aactagttgg tgggctaaga agtatggatg tcctaagttc
     5761 ccaaaacaaa atgaggatgg tacaataaca gcttaattta tattacagtg agttgttggt
     5821 aaaataataa attctttaat tagattttag gatattaata aaattatatt atttataaag
     5881 gagagcaaga ataatgataa atgcagtgat aattgatgaa aaagataatg ttgcagtagc
     5941 tattgaagca atagctaaag gtagtgaaat tagttttaaa ttaaaagaca aaacagtaaa
     6001 aacgattaca gcactagatg atataactat ttatcataaa ttggctataa aagatatggc
     6061 ccaaggtgat aaagttacta aatatggtga acatatagga gaagcaaatc aggaaataaa
     6121 aagtggtcaa catgtacata ttcataatgt tagaagtgtt agagaggatt tggataaaca
     6181 agttatgact aatatataaa ataattttat agagttataa aagttgtatt cattgctttt
     6241 agtgtcaaat agtttttaaa taggttttta gctgtttgat tagtgagtgt aataaaaatt
     6301 ataatatata aaaattataa aaagtatata gactatttta ggaggaaatc agtatgaaat
     6361 tttatggata taaaagacca gatggaagag ttggtataag aaaccacata ttaatattgc
     6421 ctacgagtgt gtgtgcatca gatacaacta gaataatagc atcacaagtt caaggggcag
     6481 ttacctttaa taatcagaat ggatgttcac aagtacattc agaccaacaa ttaactatgg
     6541 atgttatggc tggttatgca gcaaatccaa atgtatatgg tgtaatagtt gtatctttgg
     6601 gatgcgaaaa ttgccaaaat gatttggtag ttgatgcaat aaaagagaga acaaataagc
     6661 caataaaaac tcttgtaata caagaagaac atggaacttt aaaaactata gaaaaagctg
     6721 ttcgttatgc tagagaaatg gcacaagaag cttctttgct tagaaaagaa gaatttccaa
     6781 tatctgaact tatactagga actgaatgtg gtgggtctga cccaacaagt ggattagcag
     6841 caaatccatt aataggtgaa ttaagtgata aacttgtcga tttaggagca acatcaattt
     6901 taagtgaaac tactgaattt ataggtgcag agcatatact agctaggaga gcagtaaatg
     6961 aagaagtaaa agaaaaaata ttacatatag tacatagata tgaaaattca ttaaaattgg
     7021 ttggagaaga agttagagaa ggtaatccat ctcctggaaa tatagctggt ggtttaacaa
     7081 gtttagaaga aaaatcactt ggttgtatac ataaaggtgg tcatagacaa ataagtgaag
     7141 tatatgatta tgccaaacaa attgataaga aaggtcttgt tataatggat acccctggaa
     7201 atgatgcttc atctgttgct ggtatggtag caggaggagc acaaatagtg gtatttagta
     7261 caggaagagg aacaccatca ggaaatccaa tagcaccagt aataaaaata actggaaata
     7321 aaataacttt tgcaaatatg gaagataata tggattttga tgctagtcca gttatctatg
     7381 gtccacaaac aatggaagaa ttaacagatg atttgttaaa tatggttgta gatgtagcaa
     7441 atggaaaaca atcaaaagca gagtcccttg gatatactga aatggcaata gctagagtat
     7501 gcaactatgt ataatcttta agtaagaaaa taaatacata gaattttaat aaagggtgag
     7561 attatgaagt ttgtaagttt tactgaagga gaaaatgaat gtaaaagaac tggagttttt
     7621 tctaaagatg aattatttat tatagatgtt aactctttaa atctcagtag aaattttaag
     7681 gatttaaatg aattaattca aaatgtatct agtgaagata ttattaagtt gaaaactatt
     7741 ataaatgaaa gtacattcta taattatgat gtttttaaaa gagagagatg tacggtacat
     7801 gttcctatag aaaaaccaat acatgatatc ttatgtgtgg gagtgaacta taaagaccat
     7861 cttgaagaaa ctcaaactca ctttgatgat agttttgaag aaccacaaaa aacggtttat
     7921 tttacaaaac gtgtgacaaa ggcaataggt ccagaagatg agatagatgg tcattttgag
     7981 ataaataatc aactagacta tgaagttgaa cttggagtag taataggtaa aacaggtgtt
     8041 aatattagaa aagaagaggt agaagattat atctttggtt acactgttat aaatgatatt
     8101 tctgctagaa agttgcaaaa agaacatgtt caatggttta gaggaaaagg attagataca
     8161 tttacctctt taggtccatg tattttacat aaaagtgcag ttccttttcc tataaaactt
     8221 aaagtatgta gtagggtaaa tggtgaagaa agacaaagtt ctaatacagg attattcata
     8281 tcagacgttt cagacatagt ttcagaactt tcaaaaggta tgaccttaga ggctggagac
     8341 attatagcta ctggaacacc ttctggagta gggatgggat tttcacctcc tcgatatatg
     8401 agtagtggag atgtagttga atgtgagatt gagagtattg gaattttaaa aaattacatt
     8461 aagtaactgt tttaatataa ttaatattaa agctaaatct gatgtattaa tgaacatatt
     8521 aaaataaatt ttaggagttt aaattatgaa agcagaattt ttgacaccag ttgttacaat
     8581 atttgatgaa gaaggaaatt tagataaaga aggtaataaa aaagtgtatg accatcttat
     8641 agatggaggt gtagatggac tagtcataat gggaagtact ggagagttct ttaatatgtc
     8701 tatggatatg caaaaagaac ttatagattt agtaactagt catgtaaata agagagtaaa
     8761 agtgtttata ggtacaagtc gtatgtgtat aaatgaatct atagaacttt caaactatgc
     8821 acataatagt ggagcagatg gtgttatgat aataagccct tattatttct ctcttgatga
     8881 tgatagtatt gagttatttt atagtgaaat agctaagaat acagaggctg aaatatattt
     8941 gtacaattat ccagatagaa ctggttatga tttaagtcca gaacttacat taagacttgc
     9001 aagaaaacat aaaaacatag ttggatataa ggatacagta actttgatgg gacataccag
     9061 agatttaata aatactatga aaatagagtt cccagaattt aaggtttata gcggttatga
     9121 tgaaaacttt atacataatc taatgtctgg gggagcaggt tgtatagggg gattatcaaa
     9181 tttaattcca gaagtttgct ccaaatgggc tgaagcttac aataatagag atatggagaa
     9241 ggttatagaa attcaaaaat atattaataa agcaatggat ttttacttaa taaccaatcc
     9301 atttatacca gcaatgaaaa aagctatgaa tatcagaggg ttacaaataa gtgattatat
     9361 gactgctcca tttgtaaaac caaatgaaaa acaagtatgt gaaattaaaa aattgatgaa
     9421 tgaattaaat atatgttaaa tcaaccttgc aaagaggtca acaataaatt acaattatct
     9481 taataaaaat taaattaaaa taatactagg gtactatatt agtttaatag ggaaagtgat
     9541 gaaaattcac tacagccccc gctactgtat acggatacga aatcaaaatc cactgaaatt
     9601 tataaaaatt ttgggaaggg tgatggagga aaagccgtga gtcaggagac ctgcccagta
     9661 ttatataagt tcaatttagg gtggagattg taatttataa taaaatgaag tatcaattcg
     9721 tttttgtaaa gttgatatat gaacttctcc ttattaaatt aggagaagtt ttttatttta
     9781 ttaaaaggtt atacttatta aagatgactt taggataact ttaataagta tactttaaaa
     9841 agcatatatt attaaagttt tttagccact cataaaaaat aagatattgg agtgaaagag
     9901 atgaaattta aatcaaatgt tttaaagcag ttttttttaa ttgctatggt aatttttatg
     9961 gtaggaggtc ttatagggtg tagtaaaagt gacaaagaga ataaacaagc tagtactggt
    10021 aagaaattag aattaaaata tgctagtgga tttagtgttg aagatttaga agatggaatt
    10081 aaaaaagtaa ctgatggaga taatcgagaa ctgattttaa tcccaaagaa gattaaaatt
    10141 cctgaaaaat ataaaaatgc taatatagtt agaacgcctg tggacaatgt attaatagca
    10201 tctacaacac aggcttgttc tctgagaaca atagatgaac tagatagtat aaaagcagta
    10261 acaacagaag agcagtactg gactattaaa gaaattaaag atttaatgaa agatggaaaa
    10321 atacattacg ttggttcaaa tttagcacca gattatgaaa aaatagtaga cttaaatcct
    10381 gacttaacta tagtgagtag tggtttatca gaggctgata caaaatttat tgacaaatta
    10441 aaagaattag ggcttaatta tgttgtagac aatgagtata aagagcaaaa tccatttgga
    10501 agaatggagt ggacaagttt aatagctgca ttttatgata aagaagatat ggctactagt
    10561 gaactaaata aaactgtaca aaaggtagaa gaagtaagta aaaaaataaa aaacaaagct
    10621 aaacctaaag ttgtatgggc atctgtatat gaaggaagtg cttatatagc tcctaaagat
    10681 tcatatgtgg ataatatgat aaaaatggct ggtggaataa atgcatgtgc ttcaatagat
    10741 tcaaatgaag ggcgagtaag tattgaacaa ttgcatgaag ttgcaaaaga tgcagatata
    10801 tttgtatact catcaagttc agattatgca ccaaatttag attctataaa atctagtgca
    10861 cctgtattgg ctgatttgga tgttgtaaaa aacggaaacc tatgggtgtt tgctccagat
    10921 tattatcaat cagtagataa gacagacgaa ttaatagtag atttagttga gatgttccac
    10981 ccaggtacta caggtgaaaa aatcaaacat tatataaatt atgataaata gtacagagag
    11041 gacacaaaaa gataggtttt atagatttta gttaatatag ctttgctaag aggagtatac
    11101 aaatggattt tatcaataaa atgagaataa ggaatacgac aatatttata gtattaatta
    11161 taagtatgat acttactttt ttagtgtgtg tagcatttgg ctctgtaaaa attgaattta
    11221 aagaagtaat agatgttgta ttaagaggta tggataatac agaacattat aaaataatat
    11281 acaatataag aatccctaga gtacttgcaa ccatggtagg tggagcatgc ctagcagtat
    11341 caggtatttt attacagata ttttttaaaa acccaatagt tgaaccgtat atattaggag
    11401 tatcttcagg ttctacttta tcagtagctc tcatgatttt gggaggatac acattgggat
    11461 ttgagagtgt aaattcaatg agtatatttg tagcagcttt tataggagct ttaattgtaa
    11521 tgtctatagt cattttattt gcaaataagg taaagaatat aacaactctt ttaataatag
    11581 gaattatgac cggatatgta tgtagtggta taacaaatgt gttacaatca tttgcaaata
    11641 gtgataaatt aaaagaattt actatgtggt ctatgggaag tttttctgga tttacatggg
    11701 aaaaggtaga aatacttata atagttggta tattaggtct tttgatgact gtattcacaa
    11761 taaaaccttt aaatgctttt ttactaggag aagagtatgc aaaaagtatt ggtataaata
    11821 taagattttt tagagttttg atagtattta tatctagtat attagcagca atagtatcag
    11881 catttgcagg acctataggg tttattggac ttgcagtgcc acaaatttca aaaatgatat
    11941 ttaaaacatc agataataga gttttgatac cagtagtaat atttttaggc agtatagtta
    12001 cagctctttg tgatttaata tcaagagtaa ttttatcacc agtagaatta ccaataagta
    12061 cgataacatc tttattagga gctccaattg tagtaatgtt attggtgaaa aggaaggata
    12121 gggcttagac atgttgaaaa ctaataattt aagtgtagga tacaataaca aagttgttat
    12181 aagtaatata aatgtagaag taaaaaatgg agaaatacta tgtcttttgg gaagtaatgg
    12241 agctggaaaa actaccctat taaggtcctt atctaaactt atatcaccaa taaaaggtga
    12301 aatatatttg aatggtgtaa acataaattg tatatcaaga aaagcattat caaaaaaaat
    12361 ggctttagta cttacaaata gattattagg tgatttaatg actgtacaag atatagtcaa
    12421 tataggaagg tatccatata caggattttt tgggagtcta tctaagaaag atttaataat
    12481 ggtagatgaa gcattggagt ctgtagatgc cttacattta aagaaaagat attttgatga
    12541 actaagtgat ggagaaaaac aaaaggtgtt ggttgcaagg gctcttgttc aagaaccaga
    12601 aataattata ttagatgagc ctacaactca cttggatata aaacatagat tggaactcat
    12661 aaacatactg aaaaaattaa gtaaagaaaa gtcaatatca gtaatacttt cattgcatga
    12721 aatagacata gcattaaaaa gttgtgataa agttgctctt ataaaaaata ataaagtcat
    12781 agcttatggt cagcctgaag atgtagtaga tgaggatatt ataaattcgc tttatgaatt
    12841 ggatgataaa aactttaata gtcttttagg ttcagtagaa attagtaata aatctaaaaa
    12901 tgaagttttt ataattggtg gtgggggtaa agcaacacca atttatagag cttttactaa
    12961 aaaaggtata ggtctgtatt ctggaataat acatgaaaat gatattgatt atgaaattgg
    13021 tagaactatg gggataaaga tgtttacaga aaatccattt gagcctataa gtgatgagag
    13081 tttcgatttg gctattagaa atttaaatga ttccaagatt ataatagata caggatttag
    13141 tgtaggagag acaaataaga ggaatataga tattataaaa gaggctttaa aattggataa
    13201 aaaagtttat tcttttagaa atagagatga gagtaagaag tattatgaca gtttggatag
    13261 taagatagaa catatagata aagtttctca aataatcaat agtgcagata taaataattt
    13321 attataattt atgaaataaa atgtacttat gtgttataga aaaaagagta tagattataa
    13381 tttttttgaa attacaatct atactctttt gttattttgt ttatgtattt atatatttat
    13441 aaaactttag cattttatag ataaccaata gcttcctctc tagttaaaaa aaagtgaatc
    13501 cctgtagtcg attctttcca tctattttca ttaaaatctt cagctataat cgtttctcct
    13561 actctatata taaaattttc atcagcatat gcacgagcac ttttaaaaga ttctttacca
    13621 tctacactag taattgacag tacttttgcc ttatcacatc tacaagcatt tgatgtagca
    13681 gacactcttt ttgcgtcgct gggtattaat aattgtacca ttctaaaatt aaaacatttt
    13741 ttatagccta taaatgctcc tgtttcagga caatacaatt taaaaaaacg ggtactttca
    13801 tctgatatta ctccatttaa agtagcatgt tctaaattgg aattttctaa attagctcct
    13861 ctaatatctg agttagataa gtcacaaaat ctaaaacttg caccacttaa gtctgcattt
    13921 tttaatatag aattttttaa tgaacaatcc gaaagataag aattattaaa aatagagttt
    13981 tctaaatctg ctttttctaa atttaaattg ataaggtcag accacgaaaa gtcaatatta
    14041 cttaagcagt atcctcttaa gtccatattt tctaatctta attcttttaa atctgccttt
    14101 tctccagcat tattgttttt aagccataaa taatgtttct caagtgtttt ttctaactct
    14161 tgtttagaaa tagttttcat taaaatcacc ttcataaatt tatttttata tatgtagtag
    14221 caaaatagtc atatatttaa ttatataaca ataaataaaa aacttc
//
LOCUS       DAFKMX010000106        14196 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFKMX010000106 DAFKMX010000000
VERSION     DAFKMX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.106"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     regulatory      complement(217..300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(533..1375)
                     /locus_tag="LZB08_002437"
     CDS             complement(533..1375)
                     /locus_tag="LZB08_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7315933.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(1627..3411)
                     /gene="pepF"
                     /locus_tag="LZB08_002438"
     CDS             complement(1627..3411)
                     /gene="pepF"
                     /locus_tag="LZB08_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBM7315934.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3477..4142)
                     /locus_tag="LZB08_002439"
     CDS             complement(3477..4142)
                     /locus_tag="LZB08_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBM7315935.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4293..4454)
                     /locus_tag="LZB08_002440"
     CDS             complement(4293..4454)
                     /locus_tag="LZB08_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBM7315936.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4532..5041)
                     /locus_tag="LZB08_002441"
     CDS             complement(4532..5041)
                     /locus_tag="LZB08_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBM7315937.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5247..5465
                     /locus_tag="LZB08_002442"
     CDS             5247..5465
                     /locus_tag="LZB08_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBM7315938.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5483..7429
                     /gene="feoB"
                     /locus_tag="LZB08_002443"
     CDS             5483..7429
                     /gene="feoB"
                     /locus_tag="LZB08_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBM7315939.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7608..8663)
                     /locus_tag="LZB08_002444"
     CDS             complement(7608..8663)
                     /locus_tag="LZB08_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBM7315940.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8711..9511)
                     /locus_tag="LZB08_002445"
     CDS             complement(8711..9511)
                     /locus_tag="LZB08_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBM7315941.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9504..10295)
                     /locus_tag="LZB08_002446"
     CDS             complement(9504..10295)
                     /locus_tag="LZB08_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315942.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(10335..10757)
                     /locus_tag="LZB08_002447"
     CDS             complement(10335..10757)
                     /locus_tag="LZB08_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBM7315943.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10773..11252)
                     /locus_tag="LZB08_002448"
     CDS             complement(10773..11252)
                     /locus_tag="LZB08_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7315944.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(11384..14140)
                     /locus_tag="LZB08_002449"
     CDS             complement(11384..14140)
                     /locus_tag="LZB08_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7315945.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4595 a         2464 c         1612 g         5525 t
ORIGIN      
        1 ggtttatatt ctgcatctca atgtttataa aaactttatt ttcttattat aatactatta
       61 ttcttttaaa aaaatttccc ttttccaaca aatggctact ttaaaagcca aaattttacc
      121 tatcaataaa aatcttaacg actaatactc ttattttcaa attttgcaac atatttgtta
      181 attttaaaac aaaaaaagac tatgcaatat aaaaattata ttcatagccg gttgcaccac
      241 taactccata tatctctaga tgcccaataa taagatatac ttctcagttt ctaaaatatt
      301 tttattaaaa atcttttata tattttcata aatttctttt ttattctaac attgtaatat
      361 tattttgtca atatttccat attcttagag ataagatact aaaataccta ttttatctaa
      421 agatatttat atttctacat tcaataatag acaataccct gcaagttaaa gcatcatttt
      481 tatgagctta tctcacaggg tattatatgt ttactttata ttataactct tgttattgat
      541 taactttaat aaatcctttt tctgaaacaa gtgcttgtcc ttcatcactt aatatataat
      601 ctattaaagc tttacctttt tctgacaaac catcttcttt attaactaat ataaatggtc
      661 ttgatattgg atatttatta gctattacat ttgcatcagt tgcttcaaca tcatttattt
      721 ttaaactatt tactttctca tcaacatatc caaatgatat atatcctata gcattttcat
      781 ttccttcaac tgtagttttt atatttcctg aaccatcact tatttgtgca tctttagtta
      841 gttcttctga atcaaaccct acaatttctt ggaatccatc tctagttcct gaaccttctt
      901 ctcttgatac tattactatt ggtgcatcat ttcctcctac ttctttccag ttagtaatct
      961 ttcctgtata tataccttta acttggtcca ttgtcagatt ttttactgag tttgcagtat
     1021 tagttatcaa tgctatacca tctattgcta ttttagtttc ctttaatcca gcactttttt
     1081 cttcatcttt taaatctctt gaactcattc ctatttcaga tgttccttct atagcatttt
     1141 ttatacctcc agaagaacca atctgttgta cttctacact aacaccttga tttttttctt
     1201 gaaacttttc tccgatagtt tccataagag gacctattga agttgaacct gaaacagaaa
     1261 ctttgcttga atctcctgaa tcacctgaac ctcctgagtt agaactacat cctactgcta
     1321 aagctccaac taacattgta ctagctaaaa gaacgcctat tttcttttta aacattttac
     1381 ttacccccgc ttatatttta attttttgtt gtcatttttc tatattttaa taatacagat
     1441 tctttgttaa atgaatatta gagcagggtt aagtaaatgt aaatattgtt aaatacatgt
     1501 aaaaacttaa ttttatatga tttattctta attagattaa ctttttaaag ttactttagg
     1561 cagatataaa cataatacaa ctcatattat cagaagtctt cattgaagct atcaacctat
     1621 cacatcctat aattcttttt ctagtttatt aactaattca gcaaatacat ttaaggcttc
     1681 ctctatagat tcttttttcg tcatatctac accagcagat tttaattgtt ctagtgggta
     1741 ttctgaccct ccacttttta agaagtttat atatctatct actgcagtac ttccttctga
     1801 aagtatctgt tgacttaaag cacttgcagc agaaaaccct gtggcatact tatatacata
     1861 gaagtttgaa taaaaatgag gtattctcgc ccattctatt cctatttgtt tatctactat
     1921 tgcagatttt ccgtaatact tttcatttaa tttgtagtat atattagtaa attcatcagc
     1981 agtaagtggt tctccactct ctaccctttg atgtacaatt ttttcaaatt cagcaaacat
     2041 agtttgtcta tgaactgtag ttctaaattg ttctaaataa taatttaata agtatatagt
     2101 ttcatcctta gacttagaat tttctaataa ataatttata agaagtaatt catttaatgt
     2161 agatgcaact tctgcaacga atatcttata gtccgaatat aaaaatggtt gacttgttct
     2221 tgaataatgg ctgtgaacag agtgccctaa ttcatgaatt aaagtgaata gagaattaag
     2281 gtcattttta tagctcatta atatataagg atgtgaatca taacttcccc aagaataagc
     2341 tcctcctttt ttaccttcat tatcatatac atcaatccac ccttcatcaa aagcactttt
     2401 tatgaccttc aagtattctt ctcctaaagg tttaagtgcc tctaatatga tatcctgtgc
     2461 tttatcatat ggtatatcca tgtcaaattt atctgtcaaa ggaacataca aatcatacat
     2521 gtgtatttca ttcaatccta aaaatttctt tttaagctca acatatttat ttagattagg
     2581 aatactttta tctatagctg atataagatt attgtaaaca tctaaactta catcatctga
     2641 aaataaagac gcttctattg ctgaattata ctttctagtt tttgcgtaaa atatctctga
     2701 ttttattcca ccataaagcg ttgaggcaaa tgtattttta tatttctcat atgttgaaaa
     2761 ttcaccttca aaagcgtctt ttctaactct actattctta ctttttataa ataaagagta
     2821 gtttgaatga gaaagcttaa ccttattccc ctcctcatct tctatctcag gaaattccat
     2881 atctgcatat gctaacatat cgtatacatt ttctggaatt gtcgataatt ctgatgtagc
     2941 tgcaagtatt tcttcctctc gttcactcaa agtatgtggt ttatctctca atatatcctt
     3001 tatatatttt gcataaaagg atagcttctc atcttttaag tattcactta acttattctc
     3061 ttctatcgcc agtatttctg gaactatata agaagtagcc acacctaaat cagtagataa
     3121 catttctgtc ttagtggctc ttccttgatt atcattaatt ctagtatctt catgttgttt
     3181 catatgagta tatacatata aattttgaac aatcctagat gcattttcag agatatttaa
     3241 tactttatat aagttctcct tactctcaga aagcttccct ttatattctt ttacttcttg
     3301 gataagattt tttactttac ttatgtcttt ttcaatttcc tctttactgg agtacatctt
     3361 atctatcttc catttgtact tttcttctat tgtacttcta tcagttccca tatcatacac
     3421 ctctatttta atttgtttta tattttattc actttacaaa atatattgta tatattttag
     3481 tccaaataat ctacatttat tactccttct atcatattta tttctttcat aacgtcgtgt
     3541 aagtgtattc tcttagcata agatacaaaa aaattaacct tccagtattc ttcattacga
     3601 cattctattt ccatatttct tacgataagt tgattttttt caaatttatc atataaaaaa
     3661 ggtatagaat ccaaattgct taattttaat atcaatacat tcttttcttg gctaaataaa
     3721 aacgtattga ttatcctaag cacaaataat gctatcatta caaataatcc acctagaatt
     3781 gccaattcat aataaccata tccaactgct ataccaatac atgcagttgc ccacatccca
     3841 gcagctgtag taagcccttt tactccacct ttatttttta aaattgctcc tgcacctaaa
     3901 aaaccaattc cagataatac ttgagcaggc attcttgctg ggtcagcatt tgtatacaaa
     3961 ttataatcaa agaataattg aatcgaagtt attgaaacaa tacaagaacc tattgcaatt
     4021 agtatatgtg tcctaaatcc tgcaaactgt cttatttttt ctctctctaa gcctattatt
     4081 cctcctatta ttagtgctaa tagtaacctt attgctatct ccttacttcc tatatcaatc
     4141 ataatataaa accctcctga cttaattaca acttatgtcc tacagataat ttacacatca
     4201 gaaatactat tttatatctt aatattatta ttaatatatg taaaatatag ccatctgtta
     4261 atttttgata caaaaaaacc cacaactgtg ggctatttgc ttaggtacat gtatttattt
     4321 attaatatat ctattttctt acttgattct actaactctt cttgaaatat ttctggatat
     4381 tctatgtatg tatttatctc ttccctaact gcttcaatct cattttttaa cgcttctaat
     4441 tcattgttcg ccataataat caccactatt acaaatttgt atatttttat atacatttta
     4501 tcaaacaata ttatattttg tcaatattat gtcaataaaa cttactataa aaatgcttta
     4561 atttaggtat tgtatacctg gatactaagc ctacaaacat accagttact aatgatgcca
     4621 ttaacataaa tggtaagtac cctatagcaa gtatattttc tatgattaaa cttgcaacta
     4681 ttaattgacc tatattatgc cctattgcac ctataatgct tatccctatt agtgataaat
     4741 cttttatttt actcgctaaa tacataaata aaagactcag ataagctcct cctatactaa
     4801 atagtagata tgataaaggg cctgcaaata ttgatgacaa aattaccctg atagtaacaa
     4861 ttataacagt atctttgaca cctaacaaca ttaatgaaat taaagttata atattgctca
     4921 aacccaattt tgcccctgga aatataacta taagaggatt tggtatatat gtctctaata
     4981 catataaagc taaactatac cctatcataa gacctaaaaa tgtcattttt ctagttttca
     5041 ttaagttaac tcctccaaat tttatctatc atcttacatt ataatacaaa atatataaaa
     5101 acacctaatt tagactaaaa tatttttaat ctatttagaa taaatattag ttatttcaat
     5161 gtgcaattac ctctataaat cttgactttt tgtatcatac ttgttacaat tttaatgata
     5221 atgaatttca ttaggaggtg ttaataatga ctgtttacga tttaaaaata ggagaaaaag
     5281 gatttattaa tagtatagat ggagatatta aattatctaa aagattgctt gcacttggat
     5341 gtataaatag tactgaaata gaagttaaaa aggttgcccc acttggagac ccaattataa
     5401 ttagatttag aggctttgac cttgcgatta gaaaatctga tgctaaaaat atttctttac
     5461 aataatagag gaggagctac ccgtgattaa tgtagcatta ttaggaaacc caaatgtcgg
     5521 taaaactact gtttttaatc tacttacagg ttcaaatcaa tatgtaggta actggcctgg
     5581 tgttactata gaaaaaaaag aaggatttct tggaaaagag ataaaggttg tcgatttacc
     5641 tggtatatat gcaatggata ctttctcaaa tgaagaaaag gtttctaaat catatttaga
     5701 aaatgaagat gttgatgtaa tagtaaatgt agttgatgct tctaatttat caagaaacct
     5761 ttacttaaca actcaactta tgcaatttaa caagcctata gttatattgc ttaatatgtt
     5821 agacattgca gaatctaaag gagttaatat agatgccaaa aaattaagtg aagaacttgg
     5881 agtaattgtt gttccaatta tagcaaaaaa gaaaaatggt gttgatagga tagaagaaga
     5941 gattaaggga gctacgcaaa aagcttttat atatcagaaa aactttggaa gtgaaacaga
     6001 aacatataaa aaattagaat ccattttatc gagatgtaca aaaactacat atacacaaaa
     6061 gaaatctatc agtgataaga tagacaatat agtcttgaat cctatattag catatccgat
     6121 atttattggt gtattgttcc tcttatttaa atttaccttt gactgggtcg gaggaccact
     6181 acaagaggga tttgctagtc ttatagaggc ttatatttct gcacctgtta gtgatatgtt
     6241 atctaactct agcccatggt ttaaatcact tatagttgat ggaataattg gaggtgttgg
     6301 tggtacttta ccattcttcc ctttaatatt tacattattc tttggtatat cgttgtttga
     6361 agatagtgga tatatgtcaa gaacagcatt tttaatggat aaagttatga gaaaggttgg
     6421 tctatctggt aaagctttta tacctatagt tatgggtatg ggatgttctt ctccagctat
     6481 aatggcaact agaacattgg aaagcgaaaa agatagaaga gttacagctc ttattgcacc
     6541 acttatgact tgtggagcaa agctacctat atatgcacta tttgtagcta tattctttcc
     6601 acataatgct gctttagtaa ctacatcact gtatttggtt ggtatagtta tggcaattgt
     6661 agttgcatta ttcttaaata aaacagcctt taaaacagag gctgaaccat ttatattaga
     6721 attaccagaa tatagaatcc ctactataga tgcacttatt aaaaatactt ggaataaatc
     6781 aaaaggtttc ttgataagag ttgttactgt catgtttgca atgtcagtcg taatttgggg
     6841 actttcttca ttcaatgttt ttggatatac agaagatata aatgtaagct tcttggctac
     6901 tctaggtcat attatatctc ctatattcaa acctcttggt tttgatgatt ggagaacttc
     6961 agttgctatt ttaagtggtc ttggagcaaa agaaatcgta gtaaactctt taaatatact
     7021 atatggtaat ctaactgttg tcttaccaac aatatttaat ggagttactg catatacatt
     7081 cttgatattt actgctttat atactccatg tatagctgca cttgcaacta tgaaaaaaga
     7141 atatggcaat aaaatgatgt ttacatcatt tgcatatcaa tttatactag catggataat
     7201 ggcttttata gttaaaagta taggtggagt gctatttatg ggtaattcaa taatggaagc
     7261 tttgataggt ggaataatta tagtccttgc actctttatt ctgtttaata aattcaagtc
     7321 tacaaaaaac ggaaatgtct gtagtggatg ttctatttgt tctggatgtc caagtgctaa
     7381 ttcttgctca agtaaggaag acagcaataa aaaaaataat gcacactaat atataaatat
     7441 tttagtttaa aataagataa aagtgggtgt ctcaaaatta actttttagt tcatgaggca
     7501 ctctttttta tagaaaatca aatatatttt catcatttca acagtaaaaa agagcttatc
     7561 tagtaaaaaa gaggcttatc tctattttga gacaacctct ttttttgtta cttattcagt
     7621 ttcttgctac ctaacttcat gtggaaccta gtatcttttg gaacacttgg gtctacacct
     7681 cttaatgtag gtatttcccc acatattagt tgtataggta ctataaaatt aaatgtttga
     7741 tagtaaggat tatctgtgat atcaatcttt aaatttttat cattttctgt aacatctcta
     7801 cctatagcaa atacattttc cgtccaacca gaaagtacat caatcatctt agttactcta
     7861 ggttcttttc ctgtatctag tataaatata gtagaatcac tatttatagc attatatata
     7921 ccatgtataa actcttcaaa ctcataccca gttacaggta ttctcattgt ttccaataat
     7981 tttaatgctg cttctaatgt atctccatat atatcagagt gacctattat tcttatttct
     8041 ttagaattaa ctaacttttc tttgtttctt tcaatccatt gtttagagag cttatacact
     8101 gcttcaaatc tattaattgc atctaatatc ttatttattt cctcattgta cttttcacta
     8161 gatattatac ctttttctct agctatttga agacctaata acattaagtt taactttgta
     8221 caataatatc cttttgtttt agctccagat ttttcttcac cacaatttac tgttaatata
     8281 taatcagaaa tctcatctat taaagcattc ttacaacctg ccatagatgc tattttacag
     8341 cccttatctt cagctaactt catagcgttg tatgtagaat aactactccc accttgagat
     8401 acacctacta ctagggtatt ttcattatca aacttgaatg tatcttcagt tatcataaat
     8461 ggatacattt tcacaacatc tatatctaat aaattttgta aatatggttg cacttgcact
     8521 cctgagtgat agctagtacc tgaacctgtt attataatct tttttagatt tgttctctta
     8581 acttcattaa ataagctatc tgcattagat ataatttctc tcattctaac tggtgtttct
     8641 aacatataat cttgtattgt cattttatac accttcctta tctatatatt caataaatta
     8701 taattaattt ctataacaaa cctatagcat gtaaagcgat tcctgttact atagtgccta
     8761 tagttaattt tgttacactt acctttctct ttaacatgta gtatattaca aatgcaaagg
     8821 ttaaaggtaa catattaggt attattttat ctagtatatc ttgtaaaatt acttctgcac
     8881 catttaagtt gaatttcaga ggagtagtta tactcaacat agttgcaacc attcctccta
     8941 caacgaataa accaacagtt gtagccatag acattatttt ctccataagt ccctctcttt
     9001 gtattctttc aagagaattt acccctactt gataaccata cttaagtcca aatattctaa
     9061 gggcaaatgc tggtatattg tataatagta agaataatag tggtccaaag atattacctt
     9121 cttttgcaag tgatactcct actcctgctg ctataatcct aaatgttccc cagaataatg
     9181 aatcaccaat tcctgcaaga ggtcccataa gagctgtttt aacagcacta attgaacttt
     9241 catcaaattc tggattatta gcattctctt cttccatagc tgctgttata cctaatatag
     9301 caggaactac tactgtcgtt gtattaaaaa tctccaaatg cctttctatt gcttttgctt
     9361 gattttcttt cttattgtat agcttcttta ttgcaggtaa cattgaataa cagtaaccta
     9421 agttttgcat tctttcgtaa ttaaatgcac cttgaagagc aaatgaacgc caaaacatac
     9481 ttcttaattc tttttttgtt aatttattca aagtcaatct ctccttccgt taaagtattg
     9541 attgctaatt gaggattttg attattttct ttgttattga tataaaagtt tagtacaaat
     9601 gctacacata ctccaaataa agctatagca gttatatcta atccaccata agatgctaac
     9661 aaaaacccta taaagaaaaa tacagacatc ttttttgaaa gtaacatatc cataagcaat
     9721 gcaaaaccta ctgctggcaa caatgttcct gcaacaccca gtccgtctaa tataacttta
     9781 ggtattgcat ttatcaatgc tgtaacttta tcagcaccaa tcataattgc taaaaaggtt
     9841 ggaactgatg tacttaaaaa gaataatatt actggcaacc acatcattat ggtaactttc
     9901 ttaaaatcag cttgtttagc atagaaaact gccttttgag aaaagtacga atttattatt
     9961 ctaactaata ctccaagaga ttgtgctaag actgctactg gtactgctac tgctaaagca
    10021 acttctgcac cattacctga taatatagca aaagctgtac ctaatacgcc tcctgttact
    10081 acatctggag gagtagctgc acctattcct gcaattccca tccaaattaa ttcaagctgt
    10141 gcaccaatca taattcctga ttttatgtct cctaaaacta atcctactaa catacctgta
    10201 actattggcc tatcaagcat agattgacca aatatacgcc catctagtat accaactcct
    10261 gctataatag ctaacatagt agcttgtact aacatattga acctccattt taacttagtt
    10321 taatttacat taaattacat tacatttttg attttgtttt gattttctaa taactcattt
    10381 acataaacaa ttccattttt tgcttcctct aatcctgatt taactattag ttttgtatcc
    10441 tcctcaacta ataaaagtat agataaaagc attggtaaat ttactcctgt caataattca
    10501 aatttgaacc cttgtaaaag taagtttgta catatgtttg ccacacttcc accaaaaaca
    10561 tctgttaaga caacaaattc ttcactatct ttattatttg ataatatact tctaatttct
    10621 ttctcagcat catctgctga tttatccgtt gtcatacaaa aatactcaat attagttaat
    10681 gaaccaatta ttaatttact tgtgtcaaca agctctctgg acaattctcc atgtgttgca
    10741 attagaaatt ttttcattct ctttctccta aattatttta ttaaatctga tagttgtttc
    10801 tttttgtctc taggtaacat tctaaattct atatcaatcc ctaaatcacc tatttgttta
    10861 aagttattta tatcatcatc atcaacagca actaaatctg taagtttctt cttcccttcc
    10921 tcatatctta atcctcctac atttatttct tttaatactc ctcctgaatt tttagctaca
    10981 actaaagcat ctttggtatt ttttactaac actaatacat tgcttttctt tgaagaagca
    11041 aatttttcta ctatttctcc agattcctct acccccctta tatataaagt tacattgcta
    11101 ggtttcgcta agttcagtga catagctttc atttggtcat tttttgcttc atcatttgca
    11161 actagtatta catttatacc tagtgatgtt gcccatccat atgcaacctg accatgtatc
    11221 aatctgtcat caattctcat caaagatacc attttcccca aatcctccat catacattaa
    11281 aatttttatt gtttatactc tcatttatct ataaaagcaa aaagtatgcc aaatactggc
    11341 atactttttt aaatttcatt cacttatttt gtaatcttgt attttactgt ggtagaccat
    11401 agataatatt ataaatatat cctatctctg aaattggtat ttgcacacta taactctttt
    11461 ctagcacact aaatgcattt tttattagtt ttacttcact atgatggttt ttgattaaca
    11521 aatccatatc agtatactct tcaatttcag tttgtctaac taatctttca atcatacaac
    11581 ttaagtgaac atataaagaa atctttttat catttgaaag ttttttcttt tgaatatttt
    11641 ctatagcatt aattccttct tctattctct ctattatctt ttttgaatct aaaatagtta
    11701 gactatctat tactctaatt aatgaaaaat tctttacaat agaatcattt atgttcttta
    11761 ttttttcatt ttctactact ctgctaaata ttctaaaaac atcatcttct ccttttcctg
    11821 atattatatc ctctaataat ataaaatcta ccccattcac atttggattg gcagttccta
    11881 ctatagccat cacatcatac atttcaaaaa ctggttcttt atctcccata gaaacaagac
    11941 tatcataatc ataaggtatt attttgacat caactattcc aattaaactt tcggaaagta
    12001 gatgttggat ttttatagca gtacctatcc ctgtaaaaca agatgatatt atagcatatt
    12061 ctttattttt ttgaggataa tacaattgta tattaggttt agtttcttca tccataattt
    12121 ctgcaatctc ttcaatgttt ttaccttgca ttaataaatt tcctacttct agtgcagaaa
    12181 tagtggatat ttggtctata aataacattg ggctatttat cttattcttt aaatcttcat
    12241 atataagatt aagagagccc atatcaaata acacaattaa accattcttt atcctatctc
    12301 cctctaggta tctagacatt tgttttgata catcagcaat agtagcatct ataggcatat
    12361 caatagctgt aaatatattt ttacccaaca tccgattaca tacattcgcc atactactgg
    12421 cagtagcata tccatgtgct ataatagtcg cattaacttg attaaatttc attcttataa
    12481 ttgttgattt aataaatata atcattatga ttgtatctac attattaaga tacatatcca
    12541 atctattcgt tattaatgtt gatattttac ttattataga gtattctttg taactctctt
    12601 tttttaaata tattagtaat ttattttcta aagctttata ctcatcagac aattgataag
    12661 attcgtcctt caaataaaga taagacgaaa atgccaatac attattccca tcatatttta
    12721 tattgttatt aacttctaaa tttctaaata tatcctgcat tatcgatatt ataagctcca
    12781 taagtacgtt attattttca tcattatttg aaaagactaa attgtccatt agtctaatca
    12841 ttagattaga actcttttta ctaaattttt cctttgttat ttcatttttt tctagtctat
    12901 caaagtaatc taaacatttt atgaaacttt gatagattat atttctttta tttcttccat
    12961 ttgacatttc aatactaaat tgtttaggag aaaagataat gcttttgctt ttaccacttt
    13021 ggaattgaaa tatatctaca ttttctatat aaagatttct aggaaggtct tgaagagtaa
    13081 caaaaatatt tccatttctt ctttgatttt ttgcataagc tcgtccacat gcatatttta
    13141 ttacattttt acattcacct atattcccct tgaattttat ctttaaaatc gcatttaatg
    13201 taatttgagt tatctctata ggcaaaccaa gtaccttact ttcttcataa ataaaatgtt
    13261 ctacaatctt ccttttttca atagtccctc tttcttctat attgggaaca taaatattta
    13321 tgggtattct tcttaaaaag gtttgtaaaa attcagatag actttctgtt gtagcaaata
    13381 ttaatcttac cctagctttt ctaattactc cattctctcc aatcctagaa aacgtacctg
    13441 tatccataaa tgtaaataac ttttcctgac cttcactatt taatctatgt acttcatcta
    13501 aaaataaaac accaccatct gattgttcta ttagacctgc tctagattca tttgctccag
    13561 tatatgctcc ttttacatgc ccaaataata gactagacaa taactccaaa ttattataat
    13621 actgcgcaca gtttaacaca ttgtatggag aatttttttc taatacatta ctgcttacac
    13681 tatatttgta tattatcttg gctaaaaaag tttttcctac tccactttca cctgtcagca
    13741 ttattgtaag tccgccattt ggataaagta cagcggtttt tatttgttcg attacttctt
    13801 ttaagctatt atcatatcct atgatactat taaacacagt ctcttgcttt aataatttac
    13861 actcattttt aagttcatct aaattttcat aaatactttt ttttgtaggt cctactacat
    13921 catttatttt atttttaggt aaaaaaataa caggtctact gtttatcttt ataagattat
    13981 cagattttac taaatcattt aataatctac ttgattgagt tctacttaaa ttaaactttt
    14041 ttactatatc ttctgttatt atgccacttt cttttttaag ttcatcaaag ttgttaaata
    14101 atacacattc atttacaaaa cttaaaattt catccctcat gttcaacctc caatacaaat
    14161 atcaataata ctataatatt agtatatcat ttatat
//
LOCUS       DAFKMX010000107        14137 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFKMX010000107 DAFKMX010000000
VERSION     DAFKMX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.107"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            140..1531
                     /locus_tag="LZB08_002450"
     CDS             140..1531
                     /locus_tag="LZB08_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBM7315946.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1784..3577
                     /locus_tag="LZB08_002451"
     CDS             1784..3577
                     /locus_tag="LZB08_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBM7315947.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3658..5040)
                     /locus_tag="LZB08_002452"
     CDS             complement(3658..5040)
                     /locus_tag="LZB08_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBM7315948.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5298..5645
                     /locus_tag="LZB08_002453"
     CDS             5298..5645
                     /locus_tag="LZB08_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBM7315949.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5663..6697
                     /locus_tag="LZB08_002454"
     CDS             5663..6697
                     /locus_tag="LZB08_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315950.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6746..7795)
                     /locus_tag="LZB08_002455"
     CDS             complement(6746..7795)
                     /locus_tag="LZB08_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7315951.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8070..9605
                     /locus_tag="LZB08_002456"
     CDS             8070..9605
                     /locus_tag="LZB08_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315952.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9790..10476)
                     /locus_tag="LZB08_002457"
     CDS             complement(9790..10476)
                     /locus_tag="LZB08_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBM7315953.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10601..11983
                     /locus_tag="LZB08_002458"
     CDS             10601..11983
                     /locus_tag="LZB08_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7315954.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKEIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIQEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12385..13653
                     /gene="eam"
                     /locus_tag="LZB08_002459"
     CDS             12385..13653
                     /gene="eam"
                     /locus_tag="LZB08_002459"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBM7315955.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13746..14102)
                     /locus_tag="LZB08_002460"
     CDS             complement(13746..14102)
                     /locus_tag="LZB08_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315956.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
BASE COUNT         5470 a         1728 c         2334 g         4605 t
ORIGIN      
        1 aataataata aaaaaatgaa aatacaggcg ataatctatt gagtaaacat agaaaagatg
       61 atattataag tatgtgctaa aatggttaaa gcaatattaa tattaaattt aactataaaa
      121 caaaaggggg cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa gtggtcatcc
      181 gaagagtttt tggcttattt gcatgacaat tttgtgggaa agatttagtt attatggttt
      241 aacagcttta ttggtattat tttttacagc aagtgtagct caaggtggtt taggtctttc
      301 tactcaagaa gcaacatcca tgtttggctt atttacaggt cttatatatc ttacaccatt
      361 agtcggtggc tggttagcag ataaagtttt aggtcaacaa aaatgtattt ctataggtgc
      421 acttttaata gcaataggag atttcgtact atttgcttca tctacatcta ttgcaatggt
      481 ttgggtaggt ctatcaataa ttattttagg aaatggtttt tttaaatcaa gttgctcaaa
      541 tatagttggg gatatatatc ctaaaactga tgtgtcaaga aaagatgcag catacagttt
      601 attctatatg gcagtaaatg taggagcatt tatagctcca ataatatgtg gattgctttc
      661 tgataatgta tttgcagtaa gaggagcaag tggagatata gtatcttatg gatataagat
      721 ggcattttta atttgtggta taggcatgat gttaggtatg attatattta cacttcttgc
      781 accaaaatta ttaaatgaag tcggaaaata tcctgtgact aaaggtaata aaggtgaaaa
      841 aattgagtat ggaccactta ctaagttgga gaaaaatagg ataatagcaa tggttatttt
      901 atttttattt gtggtcttat tctggacagc atactatcaa acacaaagtt cttttatgat
      961 ttatgtaagg gataatgtaa atagaactct atttggattt gaaatgccag tagcatggct
     1021 tacatcacta aatgctatat tatgtgtggc tttatgccca gtattagcta ccctatgggt
     1081 taaattatct cataccaaaa gaggtgattt ctctattcca gttaaaatgg gattaggaat
     1141 gataataatg ggtgttgggt ttatagttat ggtatttgct acattagcta atggtccaga
     1201 tggaatggta aaggctagta tattgtttat agtattgact tatatattta caacagtagg
     1261 tgaattattc ttatctccta taggacttgc aatgttcaat aaattagctc cagctaagta
     1321 tgctacgttg tctatgggag cctggtatct aacaagtttt tttgcaagtt tattatcagg
     1381 aaagattgct ggttttacag ccacacttgg ttttttacag atatttggag gaatagcaac
     1441 agttttaata atatttggtt taatattatt gggaataaga aatgtattaa caagattaat
     1501 ggctatggat ttactaacaa aagaaaaata attcatgacg aattattgta aataaatgaa
     1561 ttactttaaa taaaggaagt tacatttaaa tataaaatta aatgtaattt cctctttgat
     1621 tatgaaaaaa agctttacac aaatagatat tataattaaa attataattg tgataattag
     1681 agtgtataag atttatataa attatacaaa aaatatttat gtaataaact ttacatactt
     1741 atatatttag ttattcaaac tattatttcg aggaggtctt gttgtgaata taaaagatag
     1801 attaagtgga ttaagaaaat ttatggaaga aaaaaatata gatgcctata tgattccatc
     1861 ttctgataat caccaaagtg aatatgtagg agattacttc aaatcaagag aatttatatc
     1921 agggtttaat ggttcagcag ggacggttat tgtgactaaa gacgaagctg ggttatggac
     1981 agatggaaga tattttatac aggcagaatc tcaattagaa ggaagtacaa taaaattatt
     2041 caaaatggga caagaaggtt gcccaactac agatgaatat ttatataaaa atattcctga
     2101 aggaggaact ttaggatttg atggtagagt tatatctgct agagaaggag ctacattagc
     2161 tgaaaaatta tctaaaaaag gtataaagat agaatatcag tatgatttaa tagatagtat
     2221 atggccagat agacctgctt tatcagatag taaagcattt ttattagatg ttaaatattg
     2281 tggagaaagt tttagctcaa aattagctag attaagagaa aaaatgagtg aaaaaggtac
     2341 ttctactcat gtaataacta ctcttgatga tatagcatgg ttattcaata taagaggtgg
     2401 agatgttaaa tataatccag ttgtactttc ttatgcagta ataactttaa aagaagttta
     2461 tctatttgtt gatgaaagta agttaaatga agaaatacta aatgaactag ctaaggaaaa
     2521 tgttcaaata aagccatata atgatgtgta tgaatttgta aaaaatatag acaaaactga
     2581 aaaagtttta ttagatggaa ctaagcttag ttatacaatt tacaataata taccttgtga
     2641 agttgagaaa gttgatgagt tcaatccagt aatgtttttt aaagctcaaa aaaatgaggt
     2701 agaattagaa aatataagaa attcacatgt aaaagatggt gtagctttca ctaaatttat
     2761 gtactggtta aagaaaaatg taggtaagat ggaaatcact gaaataagtg ctactcaaaa
     2821 attagaggac ttaagaagag agcaagaagg tttctttgag ccaagtttca atacaattgc
     2881 tgcatataaa gagcatgctg caatgatgca ctatagtgca actccagagt caaattataa
     2941 attagaggca gaaggattat ttttagtaga ttcaggtgga caatattatg atggaactac
     3001 tgatataact aggactacag ttttaggacc tataagtgat gaattaaaac tacactttac
     3061 ttctgtagca agaggaatga taaatctttc taaggctaag ttcttgcatg gttgtagagg
     3121 atataactta gacatactat ctagaagttg catgtggaat atgggaattg attatcaatg
     3181 tggaactgga catggtatag gatttgtgct aaatgttcac gaagctccaa atggatttag
     3241 atggagagta gtacctgaaa gatttgatag tgcagttctt gaagaaggaa tggtaactac
     3301 taatgaacca ggtatatata tagaaggttc tcatggtata agaacagaga atgaaattgt
     3361 agttagaaaa gctgaaaaga atttctatgg tcaatttatg gaatttgaag ttgttacgtt
     3421 agctcctatt gacttagatg gtatagtacc agaattaatg aataaggatg aaaaagatta
     3481 tttaaactgg tatcataagt tagtttatga taaaatatca cctttcttaa ctgatgaaga
     3541 aagagaatgg ttaaaagttt acactagagc tatatagttt gtactagata caataaaaat
     3601 cgaggtatta taatgcctcg attttttatt gtatatttta ttaactttaa aaaattttta
     3661 atttgataat tctactaatt ttttattcat tataagtagt ataattccaa atatcaataa
     3721 aaatgatggt atcactatga agacttgtaa cataccaagt tggtctataa tagcttgcgt
     3781 atagccagat aatttatttg ctgcaaattt gcctaaaaac caaacaccca ttagtaatga
     3841 aatatacttt tcaggcgaaa gtttatttac cataccatac ccgactggag aaaagcaaat
     3901 ttctcctata ctttggaatg ttaaaaatcc aactacccaa aatacacttg ctttgtgaat
     3961 tgaattaggg tctgttcctg tttgtataac tgctactatc ataaacaaaa atgctatggc
     4021 taatagtata aaacctaagc tcattttttt tggcacactt aaatcgcctt ttttagtttg
     4081 agataatttt acccatacta aagcagagat aggtccaaga ataacacaaa ggagaccatt
     4141 gtatgaatct atccatgaag taggaatttg aaaattgcca acagtaagat ttatgtgttc
     4201 ctttgtatag agtgcaatag acatagaggc ttggttatat gctatccaaa aaaatactgt
     4261 gaaaaaagct aacaccaata ttactattat tctttttttc tcaatcaaag ttaaaggtct
     4321 tgaagcgagc attttcttat attctagtga ttttcttata gactctctcc cgttaatagg
     4381 gtcattattc aaaggtttat aaaactttct gtttccaaat ataaagaaga ataaacctat
     4441 tagaaccgta attgatgaaa ataaaaatac atatctatat ccatacatac ttatagataa
     4501 tccgcttttc atagcaaacc attcattagc aactaaaccg catattaaag ggccaaataa
     4561 agtacctaga ttagtagcca tatacgtaat agagaaagct ccatcttttc ttgggtcatc
     4621 attggtatat aagtttccaa ccatagtatg taagtttcct ttataaaaac ctattcctaa
     4681 tccagataaa aatatcatga tgtttatatc catcttacta tttgctatgt atcctagaaa
     4741 ataaccaatt gaaaccatta gcattccaag tataatacag tttttgggac ctaaaaatct
     4801 atcactgacc catccaccaa taactggaga gaaataagtc catgctaaaa aagatgcaag
     4861 cattgatgcg gattctgctg gtgttaaccc taaaccacct ttggagactt gttcagctaa
     4921 aaacaataat aataccaatt tcataccata gtatgcaagt cctacaaagg taaatattga
     4981 acagcagaaa tataaatctc taggttgttt aagtttttct tgcgttttta aaactgacat
     5041 atgtcgagaa cctccagata aatagtttaa caaaatttta ccatactata tgagtatatg
     5101 tttttttcat atagacgtca acttgtaaga taatttaatt aagtcgaaat atatgtaaaa
     5161 atagtatcat ttttgtgtat tcagtaatat ttttttatga taatatatgt tattattata
     5221 ttaaaataca aacatattta tggaaaggat atgccaattt agtagttaga ttgattgttt
     5281 ggcaggtggg atgagctatg ataagaaaga aatctgaatt acaagtagaa gtaaaagaaa
     5341 atttaaatgg tggtatagga aaggttaagc ttgaaaacat aatacaaaat gaagagctta
     5401 aaggaaaagg aagactattt aaaagagtag ctttaccagt aggaagttca attggtgtgc
     5461 atgaccatac aacagatttt gaagtatatt atatactaaa gggaaaaggt aaagtatttg
     5521 ataatgggga atttgtagaa gtaaatgagg gtgatgtagt gtatacagct gatggcgaaa
     5581 aacattctat agaaaatata ggagaagaag atcttgagtt tgtagcacta gttttatata
     5641 tgtaaaatat ggaggaggag tcatgacagg caagatagaa gaaattaagg acaagataaa
     5701 tagattttgc aactccaatt taaatcaaga atacaaagat ataagcttta aaattttgca
     5761 aaacctttta gataataata aagagataat tcattcttct agagctgata tatggtcttc
     5821 agctatacta aatatagttt tagaacaaaa tcttttatac aataagaagc atccactaca
     5881 tataacaaaa aaagagtttt caaaacaaat aggtgtttct ttaaatacta taaataataa
     5941 atcgtcttta ataaaagatg tttgtagtat tgacttttta aatcaagata ctatagctat
     6001 ttctaatgta gaattttggg taaaagagac taattccaaa tcaaagtctg tagatttaga
     6061 tttagaaaag aaaaaaatac tatataaaag atatattaag ctatcacaag atgcgaatag
     6121 tgatattgaa tcaattagat atatggagga agctgtaaat ataggtaaaa gtatgattac
     6181 taaagaagat aaagaacttg gattttggaa aggtttatct accagagcct atatgatagc
     6241 acttgaatcc cttgctagaa agttagaaag tattaataat ctaaaggagt ccaaaaaagt
     6301 attagaatat ttaatagaaa ttaatcctag tgatgagcag ggaataagat acaaattatt
     6361 aaatgtatta attagattaa atgatagaac ttcaataaat aaattatttg aactttataa
     6421 agaagaaaaa tctgctacat ggatgtacag taaagctctt tattacttta aaaataaaag
     6481 tatgtttttg gcaaatgatt ctattaaggt tgctagaagt aaaaataagt acgttggatt
     6541 atatttgtta gattggcgaa atgcttttgg tagagaattt gttacagagg aagaaaaggc
     6601 agaagctgtc tattactatg atgaaaacat agtgatttgg aatgaagtta aaggttctat
     6661 ggattggcta ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc ttacttttat
     6721 ataagtaaaa ggagtagttt ttaatttata catctagttt taaatcttca ggttttatta
     6781 gacctttttt tctcataaat tctgaaatca acaaagttac aatagcagga agtataaaat
     6841 taagtaaaag tataccaacc cacatagata tacctccttt tccagcactt tgcatagcag
     6901 ttactgttcc aaattgtcca accaatccac aagtgcccat accagaacca ataggaatat
     6961 tctccatttt gaatattgtt gtagataatg gtccaagtaa tattgaagct acagtaggtg
     7021 gtatccaaat tttccaattt tttattatat taggtacttg caacatagat gtcccaagac
     7081 cttgtgccac aaggccacca actccatttt ccctaaagct cataacagca aagcctacca
     7141 tttggcaaca acaacctaca gttgctgccc caccagcaag accacctaaa cttaacatca
     7201 tacaaatagc tgcactactt ataggtaatg taagagctat tccaacaagt gcagagacta
     7261 aagctcccat tattataggt tgcatagttg tagcttgcat tattatattt ccaaatgaag
     7321 tcataaaagc agaaacagct ggaccaataa gttcagctac aagtactcca attagtattg
     7381 ttacagcagg tgtaaccaat atatctattt ttgtttcttt tgaaactaat tttccaacct
     7441 ctacagcaaa aagtgtagcg acaaatgctc caacaggtcc tcctaattca ttaccacaag
     7501 ctccaacaag tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca tacgcaatag
     7561 ccactgctat agctggacca gtaacctgtt tagcaagagg gcttataact tcagtaaaaa
     7621 gagaaatatt gaaattatct cctaatgtat tgaaaattgt tccaattaaa agggacgaaa
     7681 aaagaccgag tgccatataa cttagtgcgt caataagata cctttgaaca gaaatttcaa
     7741 tattttttct tttagcaaat gatttgaaat ttcttttttt ataattgatt tccaatttaa
     7801 aatcctcctc atataatata agtataattt ctttttaatt aattatataa cttttcagct
     7861 gacaagacaa atgttatttc actaaaatta gactattgta atagtaagaa attaacatta
     7921 attttagttt taaattttaa atttatggtt tataattagt atagggatat tctgaaatat
     7981 aataattttc cagtttatcc caatatttaa cttaggtaga ttgcttacaa aacaaatgat
     8041 tatatattag gctaaaagga agtggataga tgatatttgt aaaaaataag aaatacagaa
     8101 aaattgccat aattatatct attcctatat tgcttgttgc gcagtactac ctttttaaat
     8161 ttggaatact tagaccaatg gtaaagggag tagaaattga aattgtagat ggggaatatg
     8221 ttaaagatat agataaatat gtagtaaaat taaatgaaac agttactttg tctacaggtg
     8281 aatatattaa ggttccttcg tatgctaaaa atccaaatat ttggtttaat gtattggata
     8341 actcagggac tttaaaaata gatggaaata aaatgacagc attaaaggtt gggtattctt
     8401 ctgtgggtat catgaaaaat tcaagagtat taaaaaaggc tactataaaa gtagtagacc
     8461 cagaggtaga atcactagat atggagatta atggggattt aaaacatgtt ggtgatagtg
     8521 ctactataga aagtacaata gaagttgatt atgataagtt taaaaaatca tataagccag
     8581 tttataagag tagcaatgaa aatattttaa aaatcaaagg aaataaagta agtgcagttg
     8641 gagttggtaa agctacaata tatgctaagt gtggaaataa agaaattgag agaacattta
     8701 ggattcaagc tagagttgct gaaataaata tggatgatat aaaaatagaa gttgaccaga
     8761 atgaaaaatt gaaaccagat ataataactt ctccacctgg tttagaacca ccaaaaatat
     8821 attatgagtt agtagattca aaattacctg tagaaagagc aataagttta agtagtaatg
     8881 gaaatgttgt agggcttaga gaaggtaaag atagggtgag aataacttgt ggagataaat
     8941 ctaaagtaat aacagtaaca gtagtcaaag aaactataac gaatgactat atacaaaact
     9001 tggttggttc acataaaata gtaaacaata aagttattat taaacttgaa tgggattata
     9061 tgaaagatgt ctttgattat gatatatatc ttaaagataa gttgtcagga gaatcattta
     9121 agaaaattaa atctataact ataaatgaaa gtgatttagg taagtctaac aaggtatatg
     9181 cgactataga agtagatatg aagggtaatc aaaatataga ttttgatatt tatgtcatag
     9241 ggaaaaagga ttcagataca actaagccaa gtaatgtaat aaatattacc aatggaaatg
     9301 gaaattcaaa taatgataat aatattaaag atatgagggt agaaaatata tcagctaatg
     9361 ttgatagaga taataatgtc attagagtta actggagtag tatatctcat aaggactgta
     9421 cttacagtat atatgtaaaa aataatacta atggagatgg agaatttact ctatatcaaa
     9481 gtgatataca gggaaatgag catactataa gtattcctga aggagattta aatctagata
     9541 tttatgttgt tgcaaattat aatggaaata gctctcaaaa tagtgatgtt ataaatgtaa
     9601 gatagatttg tatacaaatt gaaagtgttg agtttattta aatattaaat taagaaaaag
     9661 gatagtatag ggtaattata ctatcctttt ttgttacaat atataggttt aaaatctcta
     9721 gtaatataaa ttataaaaag tcttatttac acaaatctat agatattttt tttattaatc
     9781 aatattgtct taaattgaac ttagtgcagc ttctattgca tgtattactg tcgtatcaaa
     9841 tagtggaata gaagtgtcat tttgtttaat taaaagacct atttctgtac atcctaatat
     9901 aacagcctct gcaccttgtt tgccaagctt atctataata gataagaaag ctttttttga
     9961 agattcagat ataattccta agcaaagttc attaaatatt gtgtcattta ctattatacg
    10021 gtcttcttca tttggaatta agacttcaat tccattatta ataattacat ttttataaaa
    10081 atcttgttcc attgtatatt tagtacctaa taaggccact ttttttattt ctttttccct
    10141 taataccgta gcagttacat ctgctatgtg aagtaatgga atatgtatac tttcttgtat
    10201 tttatctgat accttatgca tagtattagt acagataaca ataaaatctg cccctgcctc
    10261 ttgtaattta attgcagcat cagctaaaat tttggcgctt ttttcccaat ttccactaga
    10321 ttggcattct tcaatttctt gaaaatctac actgtaaagt atacatttag aagagtgtag
    10381 tccacctaat ctttctttta ttactgtatt aataacttga taatatgtaa tggtactttc
    10441 ccaactcata ccaccaatta agcctatagt cttcatatta tacctctctt atttatttat
    10501 tttgaaatag ctatttacaa atacaagtat agcatctaaa atagaagcat acaataataa
    10561 aattgtatgg agtacaatta taattaagag gagtaattat atgccaataa attcttttga
    10621 aaactatcct atgacatgga aaccaaagtt agataaccga aaaccaccaa tatataaaac
    10681 tttagcaatg ttattagaag aagatattaa aagaggaaat ttaaatcctg gagataaact
    10741 gcctccacaa cgggaattag cagattttct agatttaaac ttaagtacaa taacacgagc
    10801 atttaaactt tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg gaacattcat
    10861 ttcttcggat gtaaatgtaa gtaatacatt attatatcaa acggaatcaa aagaaataat
    10921 tgagttaggg acagttcatc ctccatatga gcaaaataca tatattattg actttattaa
    10981 aaatgtacta aaacaaccag aaatggatag gtttttacaa tacatgtctc catctggcac
    11041 atatatgcaa aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat acaccagtga
    11101 agaaaatata ttattatcta caggagggca aaatgctata tgtgcaactt tactaggatt
    11161 atttaaagct ggtgatagaa ttgctacaga ttctttaagt ttttctggaa ttaagtcaat
    11221 agctaaaatg ataggtattc aattagtccc aattcctcaa gaaaataatg aaatatcgat
    11281 tgaatatctt gaaagttatt gtaaaaatga aaatataaaa ggcatatatc ttataccaga
    11341 ttatcataat ccaacgacac atactatgtc tgatttatct cgtaaaaaga tagccaagat
    11401 agcaaagcaa tataatataa taattattga agatgctata aatagcattt ttagagacgg
    11461 tatacaaact cccatatttt ctttagctag tgataatact atatatattt ttagtacatc
    11521 taaattttta tgtgctggtc ttagagtagc atttgtagtt gctccaaaac gatatataga
    11581 aaatttagaa aatgcactat ataatatgaa tctaatggtt tctccattta cagcagaaat
    11641 agtacaccgt ttattatatt ctcctattat agataaaatt atacaagaga aaagagatgc
    11701 aattatagaa agaaatgaag ttgctgataa agttttgtca gattataatt tgattggtga
    11761 taagaattgc agttttcgtt ggttacttct tcctgatgaa ttggatggaa aatcatttga
    11821 aatttctgca aaaaatctag gagttcaagt atattgtgct gaacgatttt cagtaggaaa
    11881 ctctacagtt ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg tagaagaatt
    11941 agaaaaaggg cttaatataa taaagtcact tcttaagagt taaatttatt ttactattat
    12001 ttaattaaat aaataaaaag gcggagaaat ttattataat aaacctccgc ttttattatt
    12061 taatgaaata ctttactttt tattaaaaaa tttaatttta ataatcttga aaatataatc
    12121 gttttatggt aaaataattc ttaaaaatag aaaaaattaa tcatttgtta aaaaaaatgc
    12181 ataatttatt gtaatttatt gacgaaggtt gaaaaatatt cataaatgtg cgaaataaca
    12241 gaaaaaatgc aaaaattaat tcaatttcat ttgttataaa atctatatgg caaaattaaa
    12301 tctgcctatc agaatgatat aaacacatca gaaaggagga cgctatcaaa taatacaagt
    12361 agttaacttt gatagcaaaa aattatgaat gaacaaacta gaatatcctt agagagagct
    12421 gctgaattaa aatcaaaaat tgatgattat attcaggcta gaaaaacgat taacagaggt
    12481 cttgaaaaag aagaagagat aaataaacga aaacagaaaa tattaagtat cttaaatgga
    12541 actgaagagg attggaataa ctacaaatgg caattatcaa atagaataac agatgtagat
    12601 actttatcaa aaattataac tctaactaaa aaagaaaaag aatatataaa agaggttggt
    12661 actcaattta gatgggcaat atctccatat tatttgagtc ttatagaccc agaagatata
    12721 tgtgacccaa taaaattact gtctatacca acacatatag agttggaaga tgaacaagaa
    12781 gatttggacc caatgggaga agagtataca aacccagcag gatgtataac tagaagatac
    12841 ccggatagat taataataaa tgtaacaaat gagtgtgcta tgtattgtag acactgtcag
    12901 agaagaagaa atattggaca acaagattct cataagtcaa aagctattat ccaagaatct
    12961 atagactata tcagagaaaa tgaagaaata agagatgtac tagtaactgg tggagatgct
    13021 cttacattaa aagatgatta tttagagtgg attcttagcc aacttaaaga gataccacat
    13081 gttgattatg ttagattagg tactaggact cttgttacaa tgccacaaag aatcacagat
    13141 gaattttgca atatgctaaa aaaatatcac cctgtatata taaatactca ttttaatcat
    13201 ccaatggaaa taactaagga atctaaagaa gcttgtgaaa agttagcaaa tgcaggagtt
    13261 ccattaggaa atcaggcagt attattaaat ggaataaata atgataaatt tgtaatgaga
    13321 tgtttaaatc aagaattact gaaaataaga gtaaaacctt attatatatt ccaaagtaaa
    13381 catgtaaagg gaacaaaaca tttcaataca tcagtagatg atggtcttga aatcatggag
    13441 tatttaagag gatatacatc aggaatggct ataccaacat atatagtaaa tgctccaaaa
    13501 ggaggaggaa agactccttt gcttcctcaa taccttgtat caaaaggaac agattacgtt
    13561 atgcttagaa catgggaagg aaaagttata aaaatggaag atgaacctgc tgtagatata
    13621 aagaaactta taaaagaaca agcacaggat taaaaaaata tttaaagttt ataaaaagaa
    13681 acaactttaa aataaaaaga aaatggatgt caaatttata tcaatttgca tccatttttt
    13741 attagttatt tattattttg ttgttgatta actaattcaa gatatccact tgggtggaag
    13801 taatatatct cagaagtatt ttcatttaca aagtaaccaa tatcaccatc tgcatctggt
    13861 aaaaatacat aaccattaag atttttagct tttaatgctt caaaatcact agcatttcct
    13921 tgatatatgt aatcaacttc attgttgtat tttactaaaa gttctttagc ttgttcttga
    13981 gtcaatttag tattttggct tacaggaaca ttcttttgag aatctgcata agaagattca
    14041 tttactggtg cagttaataa caaacaagat aaaactaata aagatataag tgcttttttc
    14101 atactaaaac cctctttcat gtttaattat ataagat
//
LOCUS       DAFKMX010000108        14127 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFKMX010000108 DAFKMX010000000
VERSION     DAFKMX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.108"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            326..556
                     /locus_tag="LZB08_002461"
     CDS             326..556
                     /locus_tag="LZB08_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315957.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            613..1263
                     /locus_tag="LZB08_002462"
     CDS             613..1263
                     /locus_tag="LZB08_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBM7315958.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1466..2137
                     /locus_tag="LZB08_002463"
     CDS             1466..2137
                     /locus_tag="LZB08_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7315959.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHYIVTLIRSGYK
                     FEVKE"
     gene            2134..3264
                     /locus_tag="LZB08_002464"
     CDS             2134..3264
                     /locus_tag="LZB08_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7315960.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3696..4541
                     /locus_tag="LZB08_002465"
     CDS             3696..4541
                     /locus_tag="LZB08_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315961.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4541..5569
                     /locus_tag="LZB08_002466"
     CDS             4541..5569
                     /locus_tag="LZB08_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315962.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5572..6585
                     /locus_tag="LZB08_002467"
     CDS             5572..6585
                     /locus_tag="LZB08_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7315963.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(7074..8324)
                     /gene="cme"
                     /locus_tag="LZB08_002468"
     CDS             complement(7074..8324)
                     /gene="cme"
                     /locus_tag="LZB08_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBM7315964.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8410..9156)
                     /locus_tag="LZB08_002469"
     CDS             complement(8410..9156)
                     /locus_tag="LZB08_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7315965.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9290..10312)
                     /locus_tag="LZB08_002470"
     CDS             complement(9290..10312)
                     /locus_tag="LZB08_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBM7315966.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDIFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGSWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10312..12732)
                     /locus_tag="LZB08_002471"
     CDS             complement(10312..12732)
                     /locus_tag="LZB08_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBM7315967.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLIVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHNDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHNILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12818..13738)
                     /locus_tag="LZB08_002472"
     CDS             complement(12818..13738)
                     /locus_tag="LZB08_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7315968.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         5014 a         1816 c         2212 g         5085 t
ORIGIN      
        1 ttatatatat taagatttat tattatattg agccttatat taaattaagg actataattt
       61 gaaaataagc tataaaaact taatattgtt ttgttcaaag ataaaaaaag aatactttag
      121 gtgattttta ttaaaaatta agagtgataa gattatatac tttataaatg taaatatgac
      181 gaataaaaat gataaatata ataatatata tatttacatt aggagttttc tatactataa
      241 ttatttaaaa ctaaatataa atgttggaat tatttaggac ttgaaattgt ctaaaaataa
      301 atataagtga tgaagaggtg atgtgatgag attagatatg gatagtatgt tatatgtact
      361 aatttatatg acattattgg tatctatcat tggaagtata ttttattttt gtaaagacct
      421 cttaaatcat aaaattaagt atgttttaat aagagacata tttgtaaata gaattacata
      481 ttctgaagaa cggttgccta aacagtatat agtttttcag aaatatgata tttggaggta
      541 ttgtagtttg ttttaatttg aaatgaatct attaaaaatt ctaaagttta aaaataaaaa
      601 ggagagaata aaatggattt catttcaagt atattgcaag gattattaag tatattattt
      661 aattttacag gtgattttgg aatttctatt gtacttataa ctttaatcgt taaattagta
      721 cttatgccac tatcattaaa acaaagattt tctgtaaaaa aacagcaaga attagctgaa
      781 aaaatggaac atataaaaga aaagtataag aatgatacta aagaattgga aaagcaaata
      841 caagtaagtt ctgtagaaag tatgaagagt atgctgggat gtcttatagt tttggttcaa
      901 atgccagtag tatatgcttt atatcatgta tgtttagata tgacaaagga atttacaaca
      961 attataattc catggattgc taatttaggt acatttgata atttgtttat attaccttgt
     1021 atatatacat taattatgtt agcttcaaat ttagtgaatt atataccata tttaaaaata
     1081 aattctcaag taaagtttaa caaacaaatg gctgtggtga ctaccataac aagtcttata
     1141 ctaactgtta gaactccagt agccataggt ctgtacttta taacaagtgc aatttattca
     1201 ttaattgaag atatttgttt tagaatatat tttaatcgaa aaaatagaat agtgacaaaa
     1261 taatataatt attattagaa atattttagc ctgctaacat agaaaaagtt agtaggctat
     1321 ttttatctat gatatcaatt ccattaagaa tagtctaaaa aatactatca aaatgctata
     1381 aaaaatacta taaaaaaata gttgaaaaat aaataaatta atataaacta aaataaagcc
     1441 tacaaaaaag aggtgataaa tagaaatggc taaaatacta gttgtagaag atgaaaaaag
     1501 aatgcaaaat attattgtag aatatatgca aaagggagga tatacttgta taactgctga
     1561 tgatggagtc gaggcactta caatattaaa gagcaacaat atagatttaa tgatattgga
     1621 tattatgatg ccgtatttag atggattttc agtgtgtaga gtttcacgtg aaatgactaa
     1681 tatacctatt attatattga cagctaaagg tgaagaggag gacaaattaa agggttatga
     1741 atatggcgca gatgattata taacaaaacc atttagcccc aaagtacttt tagcaaaagt
     1801 taatgccttg ttacgtagat acactactga tattcccaaa aattcattga gtttaggaaa
     1861 gatttttatt atggtagcat caagacaagt gtatgtagaa gataaattga ttgatttaac
     1921 atataaagaa tttgaattat tgcgtttatt tatggaaaac cctaatcaag ttttttccag
     1981 agagaaactt ttaaattgta tatggggata tgattttgaa ggaaacacac gaactgtaga
     2041 tacacatata aaaaccttac gcaaaaagct tggtagtgaa ggacactata tagttacatt
     2101 aattcgttca ggatataagt ttgaggttaa ggaatgaata gtttaagaaa gattatagaa
     2161 aaactgaaaa atgtagatat ttcaaattgg attaaacaaa attttggact atattcagtt
     2221 agaaaaaaag tgtttttact ttctaaatta tcaggagcgt taattatatt attttatctg
     2281 gttgttgaag aattttcgat aaactctaaa tttgaatttt ggatttggtt tataatactg
     2341 gttatattta ttatgagtat tgatttctta ttgggaaaat ttatttcaga accaatcaca
     2401 tctataaata aatctgcaaa aagtatgtcc caacttgatt tttctaatcc atgtactgta
     2461 aatactaatg atgaatttgg agaactttct aggagcctga atacaatgtc tacaaatcta
     2521 caacaggcat tatcagacct agaaagtgca aatattcaac ttgaaaagga tgtaaataag
     2581 gaaagaatgc tattggaaca aagaaaggag ttggtagata caatttcaca tgaaatgaag
     2641 actccacttg gcattatacg tgcgtataca gaggggctta tggatgaagt tgatgaagaa
     2701 aaaaagaaaa attatatgaa tgttataata gaagaaactg atagaatgaa caatatgatt
     2761 gtatcattat tagacttatc tgcattggaa gcaggagttt ctaaacttaa cccagagcgt
     2821 tttgatttta tagagtttgt tgaaactgtg gcaggtcgtc ttttaattga tataccagat
     2881 gccaattttt attttacata tgatttgcca gagtatgaag tttttgtagt ggcagataaa
     2941 atgagaatgg aacaagtagt agagaatcta gttattaatg caaagaagca tgttgtatat
     3001 aatggtaatt tagatttatc gattacatgt gaaaatggct tgcttttatt tgaaatttat
     3061 aatgatggta ggtttataga gcaagatgag atttcaaaga tttggtctaa gttttatcgt
     3121 agtgttcaat ctcagcgtac aggaggttca ggtctagggc ttgcaattgt ttcacaaata
     3181 cttacaatgc aagggttaga atatggagtg aataatcgtg atagaggagt agagttttat
     3241 tttatgattc caattgatga ataatttagt attaatttat aaatcataag tatctgatgt
     3301 aaaatatttt tatttgtata tgtaagcatt taaagagaat aaaataaaaa aacttaatga
     3361 aaaaagctta atgaaaaagt ctaataaaaa gtttaataaa aagcttaata aaaagtcttg
     3421 ttttcctgat gaaaacaaga ttttttatta agctttttat tatttcactt cgatttcaca
     3481 agagagacac tctgatttca ccaaatagaa ataaaatagg ttaaataaat taaaaaatag
     3541 aagcgtaatt aaataaggtt acaaggggga gtacttatga aatggtatac atggttgact
     3601 attatattga taatattgct ttctattctg cttaaaatta aatttataaa atggtgggat
     3661 agacgacaaa aaaatgataa aggaggttat gatagatgat aaaagtaaga gagttaaaat
     3721 tttcttatgg aaaagataaa cagatattgc atggacttaa ttttgaagta aaagaaggag
     3781 aaatctttgg attccttgga cctaatggtt caggaaaatc cacaacacag aaaatcttaa
     3841 atggcgtttt aaaaggctac aatgggcacg tatccatttt tgggaaggaa gttaaagcat
     3901 atacagagtc tctatatcaa aagattggtg ttttatttga atttccttat ttatatacca
     3961 atttaagtgc aattgataat ttagaatatt tttcgtcttt ttatcctaaa aatcaaagga
     4021 gagacatata tgaactctta gatttgttag agtttaagaa agagtttata aataagcctg
     4081 tatcatccta ctcaaaaggt atgaagcaac gtattagcat ggcaagagca ttggtaagta
     4141 atccaaagtt gttattttta gatgagccta ctagtggact tgacccatct ggagctgtac
     4201 tatttcgtaa gattattgaa gaagaacgaa aaaaaggaac aactatcttt ttgactactc
     4261 ataatatgct agatgcagat ttaatgtgta acaatgtggc ttttattgca gatggaaaga
     4321 ttatggtaat agataaaccc aaaaatttaa aaatgaaaaa tagcaataat aaagttgaag
     4381 tggaatttgt atataatgga aatcgagata ttgaaattgt agatatacaa gagttagaat
     4441 ctggaataac ttttaaatat gatgaaatct taagtgtaca ttcaaaagaa cctacactag
     4501 aagaagtttt tattaaatac acaggaagga tgttagtgta atgtattcta aattttttac
     4561 cttagtaaaa aaagattttt ggatgatgat ttatggtaaa ttttttgtgc tgacacttgg
     4621 ctctctcata ctttattcgt gttatattaa ttttggttat gtaaagttta tggatgaaga
     4681 tagttatcac aataatgtat atctatataa tccagagaat ataagcatga gtgcatctcc
     4741 attaattaat caagtttctt ctataggtga attaaaagca aaacttgatg aagatacaag
     4801 tgcagtttgt attgatatga gttccagtaa gccgaaagta attttacatg aaagtattaa
     4861 aggagttgac aattatagag ctgactatgc attgtcatta ttatcatcag aaaaagacca
     4921 taatagcaaa atatcagaca acaatgcaat aattcaaatt aatgataagg atacatctga
     4981 gataaaacaa agaaaagaca tgacttgtga gattttattc tttgaaattg tagctattgg
     5041 atttttaggg attgcctctt tattatttaa agaaaagcag atgggagtaa tgcgtattac
     5101 tggtatttta cctgtaaaaa gaagtctttt tatattatca aagatatttg ctttttttat
     5161 atgtgaccta tgttttacag ttcttttagt agtatttaat attggatttt tagaaggaat
     5221 aaaaatactt ccacaagtat tagttcaagc tagtatttta tcaattatta tgtctttgat
     5281 tggaatagga tgctcaatgt tattgagaga ttttaagcaa ttttctctta tatatcttgt
     5341 ggttgctgta ttcataacta cacctatatt tctatctgca aatacatctt ttgaaataga
     5401 ttggattaaa tatcatccct tctatcatgt ttatagtcag cttaaaaatg cgtttttcat
     5461 gaatttttca aaagaattag tttattttgt agtgtgttct ttgacaataa tagttctatt
     5521 tttacttgta aaatatattt ttaataaaga gttggttagg gagggataat cttgatagga
     5581 ttacgttacc agttaaagag tataaaaaaa gataaaatgt gtataatttc cttcttttta
     5641 ccagttgtta tggcgatttt aataaatttt atgggaacaa ttgatatatc ttcattagga
     5701 gagatacgtt ttggtatagt gaaggataca cttcctgtac aaacagagca gtggttgaag
     5761 aagtatggta tggtaagtgt atatgacaca aaaatgcatt taatacgtga aattaaggat
     5821 attaaaacgg acacaattgg agttgttaga gatggtaaag ggataaaaac tgtgctttat
     5881 ggaaatgaga ttaatgctat aaaaaatgtt ggaaagacat tgccagagat gtttagacta
     5941 aagggtgatt taaaagatgt tgaggttaca atattgccta aagatagtat tttaaaagag
     6001 tttcaaaatg tttttattgc tatgatttta attactgcta tgtttatggg atgcaccttc
     6061 aatgcaatga atattatatc tgaaaaagaa gatggaatat catttataaa tgaagttttg
     6121 ccacagagta gaagtcagta tattatacaa aaagtatttt tgggtctttt atgtgggtgt
     6181 ttatcttcaa tgataactgt atttatatgt ttggatattt caatattaga tatgataatg
     6241 atgttgttac tcgttgtatt ttcagcattt atttcatcac ttataggttt atttattgga
     6301 aagttgtcag aaggtcttat ggtaggaatt gtttatatta aaatagtaat gattttattt
     6361 ataggtactc ctttgttaat atatcttcta ggggttaata ccagtggtat agggaattta
     6421 tgctatataa tgccttcagt tgctacattt aatggtatta tggagattat taatggtaat
     6481 gtcattatta taaaagatat tgtaattttg gctgtacatt gtgtagtatg gtttatgata
     6541 tatattattt tagataaaaa aagaaagtta tcttttagta gataactcag tataattata
     6601 attgggacaa tttaataggt gatttaatat actttgtata gtgtaattta tgtagtgcaa
     6661 tagttagttt tattttataa ttattcaata taatttagta agaagttcag tacaattaga
     6721 gctacttagt ggaaaactta ataggtactt aaactatata aatactttat tttcctccat
     6781 ttgtactata aatttaattt atgatataaa tggaggtatt attatatgat ttgataaatt
     6841 tcatcattat aacctacaag taaaggtatt attatataaa gttaataaag atagtatcaa
     6901 aatattattt gctataaaga gagggactca aaataaagat aaacctattt ttcaatgttg
     6961 aaatgataaa atatatttga tttcatataa aaaattttct tcataaatta aaaagtccat
     7021 tttaagcccc ctcattttta accagataaa tcgagttaaa ctatttaaat atattactcg
     7081 gattcatttg acttagatat tccttgcttg taaaattgtt ttgaataggg gatagccaaa
     7141 gcaaaaaata aaagtataat ccctgctatg attattaaat tattaataga aattacatct
     7201 gccattggtc cgaaaatcat cataccaaga ggaataaaac cagtatatat aataataggt
     7261 aagctaagaa ctcgacctag catttgtggt gctacatgct cttgcaacat agtcatagct
     7321 gatgcttgaa tgattggaat tgaaaaattc gtaaaaaaca taaatataaa aaattgccaa
     7381 gcttgtgtta caagacctag aataattgaa aaaatagcat aggataatat tgctagaaaa
     7441 aaggtcttat ttctattttt aaaaccacca aaaactccta taatcaatcc acctaaaacc
     7501 atacctaaaa atccaatcat ttcaataata gacaggtata tataaccctt gccaaatgtt
     7561 cgttcaataa gaagtcctat aagaaatcct gatggaacac aaagaagcat gaatgctcca
     7621 taagtaaaaa gcacttttct aatgaactta tctgaaaaac agtactttat ccctgatacc
     7681 atatctgcaa aaaatgagac tttttcagtt tgttttggta tatcatgttt aggaattttg
     7741 atgaatatca ataatacaat agcaataatt gcagtaaata catcaattaa aagaacttga
     7801 tttattttat taccaaatcc aagtattgca gcagctacaa taggaactat aaattgtact
     7861 atagattgta ggctactatt tataccatta aaacgcatta aatgttcttc tggtacaagt
     7921 tgaggaatca ttgcgtttac agcaggtgtt tgtacaccac ttcctagtga acgtataaca
     7981 gatattactg atatggctat taattcactt gttgtgccat ttgatgacat cataaataga
     8041 attagaacta atgtagatat agcaattgtt gaatctgata caattatgag tacctttcga
     8101 ttgtagtagt ctgcccatac accagcaaaa aaagaaatta tcatttgagg tataaaggag
     8161 catacagtta aaactgttac ccatacacct gatgatgtct gaagtgtgac ataccatatc
     8221 attgccattt gtacaagtga tgagccaaat aaggaaatat attgacttgc taaaaaaaga
     8281 atgacttttt tcttccatgt agaattagta tttttgtttt ccatcctatt cctcctttga
     8341 tttgtgtgtt gaatttttct tgctcttaag gataacacac aaatcaaagt ctggagttag
     8401 agtagagggc taggaaaatt gttttttcat aatttgaaga tgagatttca tggtttttgc
     8461 attatgttca gcttctttca atgaagttat cagtttgtca cacactgaga taatttcgtc
     8521 atcatctttt ggagtgtcaa ggacagtttt aatattagta ttaggattag aagataaagc
     8581 atttaacatt cgtaaaatag ctgaaagaga atagtttgca caacgaagtg cgcttataat
     8641 tttaagtcgt tttatatctt catcagtata tacacgataa ccattttgtt ttctttttac
     8701 ttttaacaaa ccattcattt cccaatttct taaagtatct attgtaacat ttaagtgttc
     8761 tgcagtttct tttctagtta aaaagagttt atttgtttct ggagtatttc catttaaaat
     8821 ctgtgcaaca atagtaagtg cttcctcggc attagatttt tctatttcta attgttgcaa
     8881 gtattgattt acaagactaa tagccttatc aaagtcccta ttcgctgatg ttcttacaat
     8941 ctcaagggca tcttttctta atcccctttg taccacttca attttcaagg ctgtacgagc
     9001 aaatctaaat tgttctatat gaaaatcatt aaagatacga taaccattgg tattacgctt
     9061 aggtttaggt attaagttta gttcttcata taaacgtact gtattaggat gtacaccaat
     9121 taaatctgct acctctgatg tcgtgtaagt gtccattagt attcaactcc ttttagatat
     9181 atagtattag tcataataac accaaacaaa agtctggtgt tataatggag ggtttggtta
     9241 tctaagagta attagtttat gaaagagtaa ttaatttatg aaagagtaat taatctatta
     9301 tagaagtaag tatatatttt tcaataaact cccatctatc acgaggttta aacactattt
     9361 tagatggtat ggctattact aagtcatatt gtggaataca acaaatagca tttcctccag
     9421 ttcccattgc tgaataccac aaaacaccat cttttttaca tagccaccac agataaccgt
     9481 attcattact attcatttta actgattctt caatccacga tttagatata atacgtttat
     9541 tattccaaat accatcattt aaatacagaa aaccaaatcg caacatatct ctagcactca
     9601 atgtaagtcc ccagctacct atagaataac cctgtctatc ttttgcccaa cccttaagat
     9661 tttttccaaa gaaatcttca aaaccaaaat ctttcatatc ataatctggt ataactttca
     9721 taccaatagg tttaaataag tattcattag caaattcacg agcagatttt ccagtagttt
     9781 ttgttataat agctgaaagt aggtgcgttc cagctgaaga atatttgaaa gtaccaattt
     9841 cgcctttttg accaagttga ttcaaggtat attttatcca atctttttgt ttgcaaagtc
     9901 tttctaaaga ctcagtcata tttttaaaag ggtatggtgc agtcattgta agtaggtgac
     9961 gtatagttac tgctttttta catatttcag aaggactaca agtatattca gggaaaaaat
    10021 caacaacctt ttggtcaaca tcttttatat atcctttatc tattgcaata cctattaagg
    10081 ctgatataat actttttgta actgatgcaa catgaaaaat atcatcagca ttatatccat
    10141 tatagtactt ttcaaagaca acttttcctt ttcttgcaat aattattcca tttatattac
    10201 tatattgtga gattattaaa ggttcaagtt gtgagagctt ttctgaactc ataccaacac
    10261 ttgaaggatt tactttttca aatattaaat ctaagcaatt gtttttattc attaaagata
    10321 cctccctgtt atagattttg gattttcttt tagagttttt ggagaacctt ctgcaattat
    10381 ataacctcct ttgtcaccac cttctggacc aagctcaaca atccagtcac aaacatttaa
    10441 tacatctata tcatgttcaa caacaattac agaatttcca ttttcaacca attcatctaa
    10501 taattgaatc aacttagaag tatcgtatag acttagacca gtagttggct catccaatac
    10561 ataaaggata ttaccatttc gttttttacc aatttctttt gcaagtttga ttctttggga
    10621 ttcaccacca cttaaagttg gtgtaggttg gcctaatttt atatacccca tacctatttg
    10681 ttctaataca tttaagtgtg aaacaatatt tggaatatcc ttgaagaaat gtacagattc
    10741 agaaacactc atatttaaaa tattatgtat atttttattt ttatatttaa tagataaaga
    10801 ttcatcatta aatctttttc cctgacattc actgcatata tggtctatac ttagattatt
    10861 tcccaaccag accttttcat atccactacc actacattta ggacaagcac ctttggagtt
    10921 aaatgagaaa tgaccagctg taaatcctcg ttctaacgat tctggctgac ctgcaaatag
    10981 tgtacgtatc ttatcccata taccaatata agaagcagga gttgagttca tatttttgcc
    11041 aataggagat tgggatatct ctgaatatcc agaaatatgt ttagtaccaa tcagtctatc
    11101 tgcaatagtt tcaacaactg cataatcgtc ttcatcacct atttcgctct ctgaagaatt
    11161 tgtttgatta ttgatagtat ggttattaaa ttggcttcta agaagaggaa gtagggtgtc
    11221 tgatataagt gaactcttgc cactacctga ttttccagat attccaacca tagcacccaa
    11281 aggtaatgat acagttacat ttttaagatt atttgtattt gcattttgaa tagttataca
    11341 aggtatttta tcattatgtg taatgttttt attgcttgtt ctattaggca ttggatattt
    11401 tccagataaa tactgcccag taagtgattt gtcggattga agtagacctt ctaaatctcc
    11461 ttgatatact aattgaccac cttcaacacc agcttttgga ccaatatcaa taatatgttc
    11521 tgccatctca attgtgtttt tatcatgctc aactactatg accgtattgc ctaaatcttt
    11581 aagtttcttt attgatttta agatgtctat tttttctgat tcatgcaatc cagctgttgg
    11641 ttcatcaagc acataaatta atgagtccat ctctgaatcg agatgtgaat ttaaaaatag
    11701 tctttgcatt tccccaccac tcaaagttga catttctcgg tataaggata gatgacctaa
    11761 tcgtgattct attaaatgtt gagttttgga taaaatatca tttacaagat ttgtaccaaa
    11821 tgtgtttaaa ttaccttgat ttattaagag tttaaggaaa tcatcaattt ctaaaatcgt
    11881 catttttcca agttctccaa tatgttttcc gtttaaaagt actctacgtg cttcttcacc
    11941 aattttaaaa ccattgcaat ctgaacaggc agtcatagta taaactccat ttaaatcttc
    12001 gccccttttg taacgattgt gaaatacctt tgataaagag gaactttttt taccattttc
    12061 ataatgacca tagagtactt catttcttat ttcttcaggt agttgtaaaa attgtatgtc
    12121 taaataatcg ttgaattttt tagaaaatac tctcaaatat cctggtgtaa tctttgctct
    12181 atctaagact tgttttaggg tgtcatgctt atcaaggact aatttttcaa tattaatctc
    12241 aaaatatgca ccatgtccag agcacttcat acacattcca tctgaagagt tgtatgagaa
    12301 ataacttggt cttaatcgtt cttcctcata tccacaatta ttgcaaatta gattatcgct
    12361 tacaggagta tcacacatag tacacattat ttgaccttcc actgaataca atagtgtaag
    12421 catgttgaga atattggttc ttgtgcctac tgttgaacga ggattgctct ggcgaataat
    12481 attttgttga acagctatag tgggagctaa accttgaata ttatcaaact tatcttcact
    12541 atcaatacct gcaagtatac ctaaagattg cagatattgt ttacgccctt cctcaaatac
    12601 aatgtcaaac ataaggctag acttacctga gccactgaca cctgttgcaa caataagctt
    12661 atctttgggg atagaaacat ctatattttt aagattgtgt atctttgctc cattaatttt
    12721 tatttctttc attggttcac ctcattttat caattttatc aaaccgtaaa atagggatat
    12781 attaatcctt aatacaagaa tagattaggt gaggttatta aaaaggttct ataggtaaat
    12841 atatatcgac caagtgtcta ttttgagggt gattgtgtgg gttgttaaga tatacttcaa
    12901 aagggaagga ggcacgtagc ttataattac catttgatag ccattcatta tacataaaat
    12961 cccacgcatc actatattca gattttgaaa tatcaaaatg tcccacaaga tattttccag
    13021 aaggtattat catattgcca agattgtcat tctctttgac aactatgtta tctggaatag
    13081 tcatgcaaat actagtcctt aattgatgct cctgtgtaaa ttgtggatta tcatgataaa
    13141 tggctaatat ttgtgtgttt tcaagtccaa gtagattctg ctcacttgca tatgtgaata
    13201 accgttctaa aagttttggg aaagtactgg ttagattacc atatgaacca gtatacctag
    13261 tgtaaatagt attcatatta tcaagttcta gaatttcaat ttcaccttta acctctctag
    13321 tattcttaca tttaggtttt gatatgcttc tattgtactg agaaatttta tatggttctt
    13381 tgcaattctt gctataattg tttctatatt ttatagggct tattttatag taatttttaa
    13441 atgctcttga gaaaactgct gaatcagtaa agccaaagta gtgtgcaata tcagtaacag
    13501 tcatgtcagt tctatgaata agaaaagcag ttgcattttc tagttttagc ctatttacat
    13561 attgtgccaa agtttctttt tctattgctt tgaagattct atgaaaatga aattttgaaa
    13621 aacctgctat attagaaagt tcatcaagag aaagaggttc atgtaaatgt tcttctatat
    13681 agtcttgcac tttgcaaata cgatttagat attctttttt acttttagca tttgtcatat
    13741 atagccctcc ttattgtgta aaatgtgtat caattaattt atagcaatat agtaataaaa
    13801 ttttatgttt ttgtaattta tgataaataa gatagcgttt aattttttat aattgataaa
    13861 agatatttat aattaataaa agatataaga tgccttatat ttatctttgc atataagtat
    13921 agtcttcgta ataattagta taccatagtg atttattttt aattgtgtat ttatataaat
    13981 acacaactta tgatatggac gaattattat gcaaataagc tgtaattaaa taaattgcaa
    14041 tataaaccag ttgaaacata agttagggaa atattatcaa agttattatt tagcataaga
    14101 gtatagatgg aataaaataa gattaaa
//
LOCUS       DAFKMX010000109        13862 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFKMX010000109 DAFKMX010000000
VERSION     DAFKMX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.109"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            82..222
                     /locus_tag="LZB08_002473"
     CDS             82..222
                     /locus_tag="LZB08_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315969.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(397..717)
                     /locus_tag="LZB08_002474"
     CDS             complement(397..717)
                     /locus_tag="LZB08_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBM7315970.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(837..1574)
                     /locus_tag="LZB08_002475"
     CDS             complement(837..1574)
                     /locus_tag="LZB08_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBM7315971.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1789..3612
                     /gene="acd"
                     /locus_tag="LZB08_002476"
     CDS             1789..3612
                     /gene="acd"
                     /locus_tag="LZB08_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBM7315972.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3827..8125
                     /locus_tag="LZB08_002477"
     CDS             3827..8125
                     /locus_tag="LZB08_002477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBM7315973.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8329..8790
                     /locus_tag="LZB08_002478"
     CDS             8329..8790
                     /locus_tag="LZB08_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBM7315974.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8820..9974
                     /gene="nusA"
                     /locus_tag="LZB08_002479"
     CDS             8820..9974
                     /gene="nusA"
                     /locus_tag="LZB08_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBM7315975.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            9981..10262
                     /locus_tag="LZB08_002480"
     CDS             9981..10262
                     /locus_tag="LZB08_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBM7315976.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10252..10563
                     /locus_tag="LZB08_002481"
     CDS             10252..10563
                     /locus_tag="LZB08_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBM7315977.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10585..12525
                     /gene="infB"
                     /locus_tag="LZB08_002482"
     CDS             10585..12525
                     /gene="infB"
                     /locus_tag="LZB08_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBM7315978.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12568..12948
                     /gene="rbfA"
                     /locus_tag="LZB08_002483"
     CDS             12568..12948
                     /gene="rbfA"
                     /locus_tag="LZB08_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBM7315979.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12929..13606
                     /locus_tag="LZB08_002484"
                     /pseudo
     CDS             12929..13606
                     /locus_tag="LZB08_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5536 a         1587 c         2610 g         4129 t
ORIGIN      
        1 agtttttgga aataataata acagatatta taattaatta acagtttttg taagtatttg
       61 acaatagaga gggtgtaaca aatgggaata atagagtcag catcaaaatt ggctgagatg
      121 gttcatttat tggctgttga aaaggggata actgatatag aagcatggga tgaagctgtg
      181 aaagaataca gcaaaattta tgaagaaaga agaaatgaat agaatgataa aaaatgaaaa
      241 cttgatgata aaaaataact ttatttaaag aaattcaatt tttatagaag tattaaaaat
      301 aatataaata aaaagatggt atttcaaatg taagtaaaaa atcattttgg gataccatcc
      361 ttattattta ttttaataat atataagtaa taaatcttac ttatccatat tttttatgta
      421 acctcttttt tctagctttt ttatatgctc cttacgtatt tcatcaagat taataccata
      481 ttgttcttct agcttaaaca tcatagtaac acatgtctgc gctacatcca ttagttcctt
      541 agcagttttc tttataatat caacttcatc taaatcatta ttttctccac ttagatttct
      601 gaatttacct atacattcag ctaattcacc ttgttcttca cccattttga taaatgtaga
      661 ttctagtgtt gggtctaaat tattaagctc tggtaaagat atagttttta gtttcataaa
      721 agctcctttc aagttattcg tatttatact aaaacatcat aacaaaaata atactaaaaa
      781 aaaactttaa attcaacata aaaacttaca tatataataa aaatcgtgaa ataaatctat
      841 atttgtctta atacagcctc atataaaact tctccaatag gtttatttat tacaagagaa
      901 gctgacttat catagactgt atttgcttta ttaataagag caatataatc acctttgtaa
      961 taatctataa aacttgctgc tgggtacaca gctaatgatg tacctcctat aatcaataaa
     1021 tcagcattac ttatagcaga aattgtttta gtaatgacat cactgtcaag agcttcctca
     1081 taaagtacta catctggttt tactatagaa ccacagttgt cacagtaagg aatattacct
     1141 cccagattta acatagattc taagtcaaaa aacttaccac atttagtaca ataatttcta
     1201 tgtacagaac catgaagttc tagcacattt ttacttccag ccatttgatg aagtccatca
     1261 atattttgag ttatgacagc tttaagctta cccatttctt cgagttttgc taaagctata
     1321 tgagcattat tgggttttgc attaggatat attaatttat ccttataaaa ttcaaaaaat
     1381 tcttctggat atctaacaaa aaaagtatgt gaaactaatt gttcagcagt aaattgttta
     1441 ttgagttttt ggctaaataa tccagtagaa cttctaaagt ctggaatatt agattcagtt
     1501 gaaacacctg ctccaccaaa aaatacaata ttattgtgat tagctattaa gtcttttaag
     1561 ctatttgcat ccatagttac acctccaaat tattatatat aaattgtact acatattagg
     1621 tcgtttatgt tttattaatt taaataaata tatagtttaa taagttaaat aaagggaata
     1681 taaataaaat gtatacagag tataaataga caatatttac taaaataaca agttgtcgta
     1741 taattatatt aaaattgttt tagtgtataa gaagggcggg ggaagtacgt gaagaaaaaa
     1801 gcagctttag caacattggc tatgttaccc ttaggtgtgg taaatgcaca tgctgatgga
     1861 gatataggta tagtgactat aaattattta aatgtaagaa atgaaccaac tgccgaaagt
     1921 agcatagcct ttgttgctaa aaaagatgat aaagttttga ttaaagactc ttctaatgga
     1981 tggtataaaa taaaagctga atctggacaa gaaggttggg cttcatcaaa atatatagca
     2041 aaatcaaata gtgactcttt aagaacgtct acaaataaag aaaaacaagt tatttcaaat
     2101 agtttaaata tgagaaatgg agcaggtaca agttacagag taataactgt actaaaaaaa
     2161 ggtcaaaaag tagaagtaat atcagagagt aatggatggt ctaaaatcaa atatgatgga
     2221 agactaggat atgtatctag ctcttattta ggagacgttt caaactcaac taataaatca
     2281 aaaactaaac aagtgaatac aacttcacta aatgtaagga gtggaccaaa tacaagttat
     2341 ggtttgttag gcaagttgcc aaaaggaagt aaagtagaag taatatcaga aagtaatgga
     2401 tggtccaaaa taaagtataa tggaaaagat gcctatgtat ctagtatgta cttatcggat
     2461 gtaagtcaaa gtaattcaga caattctagt caaagcaatg ataaaaagaa tactgataag
     2521 gtcgtaaata cagcttcttt aaatgtaaga agtggaccag gctctacata tagtaagttg
     2581 ggaaaagttt ataaaggaag taaagtaact gtactatcag aaagtagtgg atgggctaag
     2641 attaatttta acaataaaga agcatttgta gtaggcaatt atttatctac ttcagcagat
     2701 acttcaaata ataactcaaa tagtaactct gataacagtt ctaatagtaa tggcaataat
     2761 tcttcatcat caggtcaagt aaatggtatg tcaggtataa gtggggctaa aattgattat
     2821 aaatctttga gttatacttt agaatctcat ataagtaaac aagttgagaa agcagcatca
     2881 ggaggaaatg tgatagctcc aagtaatagg aaaagcactc caagtcctga atttagtact
     2941 ttttcagctc aaagaacaag ctcatttgta aatgcaagtt caagtgatat agaatattat
     3001 ttaaatccta aaaattttac aaatactact aaaggtatga tgcagttttt aaagattaac
     3061 agttatagag atggtatttc agaatcaagt cttaactcgt atcttaatgg tttatcttct
     3121 agtgtattta aaaaccaggg agcagccttt ataaatgctg ccaagaagta taatatagat
     3181 gttgtatatc tagtatctca tgctatgtgg gaaactgctt atggtaagtc tacacttgcc
     3241 caaggtcaaa cattaacatc ttataaggga caacctctta gtaagccagt taaagtatac
     3301 aatttttttg gtataggagc aatagataaa agtgctaatg tttcaggtgc agaagcagcg
     3361 tattccaatg gctggactag tgtagaagct acaatagatg gctctgcaaa gtggatatca
     3421 caaaattacg taaatagttc taagtacaat cagaatacta tctacaagat gaagtggaat
     3481 tatgattata catggcatca gtatgcaact gatgtgaact gggctaatgg aatttctgga
     3541 attatggaaa atttaattgg tctttatgga ggaggaagta gtttggtttt tgaagttcct
     3601 caatacaagt aatacataaa taaaaaatat agactatggg tagttttcat tttatttaaa
     3661 ttatctatag tctttttgta tataatttat aatctgtaaa tttaatgcta acaaaacttt
     3721 tatgttataa tattaattct aagtaaatat aagtgagtaa taggtagaaa tagataaaaa
     3781 gtggtaataa tatatataaa gataattttg agaaaggagg atatatatgg aaagtattaa
     3841 ggaatatttg gataagttag aaattaataa tagtggtctt ggaaagcaac ttaaagaagt
     3901 ttatataaat agagtagttt attttaagga agataagata gtttattttt atctaacttc
     3961 taaagatatt gtttctcatg aacttttaga taaatttaaa gaagaattga tgtataaact
     4021 cgattatttt aaagatatga ggatgaagat aagatttact ggtcttgaaa gaaaatccaa
     4081 taaagatgtt ataaaaaaat attggaataa tatattgtat atattaaagt atctttgtcc
     4141 atctattgca gggtggtata aacaagtaga atttctttgt ttagaagaag aattaaaaat
     4201 aaaacttcca aaaggtatat tttatgagcg attaatgaaa aaaaatgtaa cttatgtact
     4261 taaaaccgtg cttagcgaag agttgggatt agatttaaat ataacaatag aaaaagctgt
     4321 tgatgaaaag gtaaataaag aaagactcat aagaatcaat gatagagaaa tggaagaaaa
     4381 aattagggct ttagaaattg gaaaagttaa taactgtgaa gaaaatgaag aggaaagtta
     4441 tgtcattaag tctgatgtag atgaaaattt aatctatgga gataatgcaa atgctatgat
     4501 tgaaaatata gttgaattaa atgcatcttc tggaacagtt gcagttgttg gagatatatt
     4561 tgatgttgac actaaagaac ttaaaaacgg aaaaatactt atgatagctt caattacaga
     4621 ttatactagc tcaataagct gtaaactttt tttaacagat acaaataagg atggagtact
     4681 tgaaaatgtt aaaaaaggag catacttaaa aatcaagggt gacatagttt atgatacata
     4741 tcaaagagaa attagtatga tgattagtgg gataagaaaa gaaactagaa tagagagaga
     4801 agataaatca gaggaaaaaa gagtagaact ccatgcacat acacaaatgt cttctatgga
     4861 tgctatttgt tctgttaaaa agcttgtaga gcgtgcagcc aaatggggtc attctgctat
     4921 tgctataaca gaccatggtg ttgtacaagg atttccagat gctatgaatg caggtaagtc
     4981 aaataatata aaaatacttt atggagtaga agggtacttg gttgaagatg attctctcat
     5041 tatagaggat gcaaatgata aagaactttc gcagacattt gttgtatttg acatagagac
     5101 aactggtttt tctaatacaa atgataaaat aactgagatt ggtgctgtaa aaatagaaaa
     5161 ctttaagatt gtagatagat ttagtgagtt aataaatcct gaaaaagata tttcttataa
     5221 gatacaggaa ttgacaggaa taactaatga attaattaaa gacaagccaa ccattgaaga
     5281 agttcttcct aagtttatgg aatttgttgg agatagtgta ttagtagcac ataatgcaga
     5341 gtttgatact ggatttatat cgcaaaaatg tagggaacaa ggcttgttat ataataacaa
     5401 aaaagtagat acacttatgt tagcaagagt aatgttgcca aacttaaaaa gacatagatt
     5461 aaatgtcgtt gcaaaagaac ttggtattcc tcttttaaat caccatagag cagtggatga
     5521 tgctgaagct acagcactta tatttaataa gtttttgcag atgcttacag aaaaaggtgc
     5581 aaaaactcta agtgatgtaa acaatatact tggaaagatt gattacacaa agcttagcac
     5641 aaatcatata accttgatag caaaaaattc tataggtata aaaaatttat ataaaataat
     5701 ttcagatgca catgtaaatc atttttttag agcaccaaga atccttagaa gtgtattaaa
     5761 tgaatataaa gaaggattaa taattggttc ggcttgtgaa gctggggtgg tttttcaagc
     5821 tgtaaagaaa aacgtgtctg atgaggaaat gaaaaaaata atagatttat acgattatat
     5881 tgaagtaatg cctatagata ataatagatt tatgattgat aaaggagaag taaaggacga
     5941 agaagagtta agggagttga ataggaaact aattgataca gccaaaaaat ttgacaaaat
     6001 tccagtagct actggggatg ttcatttttt agacaaacat gaagctgtct taagaaaagt
     6061 tcttaaatat tctcaaggtt ttaaagtaga tgaagaagaa acttatttac attttagaac
     6121 aacagatgaa atgttggaag agtttagtta tcttggagaa gaattggcat atgaagttgt
     6181 agttgaaaat agtaatctga ttgccgatat ggttgaagat ataaaaccta taccagatga
     6241 tacttatcct ccaattatag agggctcaga tgtggagtta agagaaatgt gttacaaaaa
     6301 agccaaacga atctatggag acccaatacc agaaatagtt caaaaaagac tggatagaga
     6361 attaaattca ataataagca atgggtatgc agttatgtat attatagctc aaaaactggt
     6421 tgcaaaatct ctttcagatg gatatttagt tggttcaaga ggctcagttg gctcatcttt
     6481 tgcagctact atgagtgata taacagaagt taatcctctt ccagcacatt atatttgtga
     6541 aaacgaagac tgtaaatatt cattctttta tgaaataggt gaatggggct caggtgttga
     6601 tttaccagac aaggattgtc caaagtgtgg aagaaaactt gctaaaaacg gtcatgatat
     6661 tccatttgag gttttcttag gttttgaagg tgataaggaa ccagatatag acttaaattt
     6721 ctcaggaaat tatcagccaa caattcataa atacacagaa gaactatttg gagaggggta
     6781 tgtatatagg gctgggacta taggtactgt agcagaaaaa acagcttttg gatacgcaag
     6841 aaaatatgtg gaagaaaata atataagtgt accaaatgca gaagtactta ggctttcaaa
     6901 tggatgtact ggagtaaaaa gaacctcagg acaacatcct ggtggagtta tggttatacc
     6961 agactataaa gatgtatacg attttacacc tatacagtat ccagcaaatg atacttcttg
     7021 tggtgttata actactcact ttgattatca ttcaataagt ggtagaatac ttaaacttga
     7081 tatacttgga cacgacggtc ctaccattat aaggatgctt gaagatatga ctggaataaa
     7141 tataacagaa attcctttag atgataaaga gactatgtct ctatttacat ctacagaagc
     7201 attaggtgtt acaccagaag aaataaactg tcctataggt tgccttgctg taccagagtt
     7261 tggaactaag tttgtacgac aaatgttact tgacacaaaa cctactacat ttgcagaact
     7321 tgtacgtata tctggtctct ctcatgggac agatgtttgg gtaaataatg cacaagactt
     7381 agttagagaa gatatagttg gattaaaaga tgttatatct acacgagatg acataatgaa
     7441 ctatttaata tttaaaggat tacctcccaa aatgagcttt acaataatgg agagtgtaag
     7501 aaaaggtaaa ggtttaaaac cagaacacat tgaagaaatg aaaaaaaata atgttccaga
     7561 atggtatata ggctcatgta aaaagattaa gtacatgttc ccaaaagcac atgctgttgc
     7621 ttatgtaatg acatcattta gaattgcata ttgtaaagtt cactatccag aagcatttta
     7681 tgcgacttat tttacgacaa aagttgagga ttttgatgca gatttaattg ttaaaggatt
     7741 agatgctata aaatcaaaaa taaatgaaat tgagtcactg ggaaatgatg caaccacaaa
     7801 agaaaaaggt atgttgaccg ttttagaagt tgctcttgaa atgtatgcta gaggaataaa
     7861 attattacca gttgatatat ataaatcaga tgcaactgag tttatagttg taggagaaaa
     7921 aacattactt ccaccaatgg cagcaataca aggtcttgga ggaaatgcgg ctataaacat
     7981 ccaaaatgaa agaaaaaatg gtgaatttat atcaaaagaa gatttgagaa agagaacaaa
     8041 aatatcaaaa acggtaatag aaactcttac taatcatggt tcacttgaga atatgagtga
     8101 gagaaatcaa ttgtcattgt tttaagttag cccaaaaagt tgcaaataaa agaattttat
     8161 gatatcatag tatagaaaat agtatacttt gcagaaacta agagtgagac agctgttctc
     8221 actcttttat tttaaatata ttattaaaaa gctggtttat ttttattaaa tatattttat
     8281 ttaagattat ataaactggt catttacaat tgaataggag gaactttaat gaaaaaaaat
     8341 ttagaagcta caattgagga gattgtaact aaaataacgg atgaacatgg atttgaaatg
     8401 gttgatgttg aatatgtaaa agaagcagga gaatactact taagagtata catagataaa
     8461 gaagaaggaa tatctttaaa tgaatgtgaa ttagttagta gagaattaag tccaatacta
     8521 gatgaaaaag accctataaa agaaaattat tttttagagg tatcttctcc agggcttgat
     8581 agagcattaa aaaaagacag agattttgtt agatatcaag gtagagatgt agatttaaaa
     8641 ttatataaac cattaaatgg atgtaagcaa tttgaaggtg aacttgtagg attaacagaa
     8701 gataataata taaaaatcat tgcaaatggt aaggaaatgg aatttaatag aaaagatgtt
     8761 gctattgtca gattagcaat aaagttttag tggtatatta aatagggagg atataaaaaa
     8821 tgaatcatga atttatggaa gcattagatg aattagtagt tgacagaggt atagataaag
     8881 aaatacttat agatactata gaacaagctc ttttaactgc atacaagaaa aactttggtt
     8941 cagcacaaaa tgttagagtt gaatttgata gagacaaggg agatataaaa gtattttctc
     9001 aaagagttgt tgttgatgaa tcagatttat atgatacttt cttagaaata gagttagcag
     9061 atgctagaga gataaatcct aattatgaat taggtgatat tatagaacat gaagttacac
     9121 ctaaaaactt tggaagaatt gcagctcaaa cagcaaagca agtagttgtt caaagaataa
     9181 gggaagctga gagagaaata gtgtataatg aatttataga aaaagaaaat gaaatagtta
     9241 caggagaggt tgctagagca aataaaaatg ttgtttatgt aaatcttgga agaatagaag
     9301 gtataatgac tcaaagtgaa caaataccat ttgagaatta taaagcaggt caaaaaataa
     9361 aattctatat acttgaagtt aaaaagacta acaaaggacc tcaaatagtt gtatcaagaa
     9421 gtcatccggg tctagtaaag agattgtttg aagaagaagt tcctgaaata tatgaaggaa
     9481 tagttcaaat aaaatctgta gcaagagaag cagggtcaag aactaaaatg gcagtaagat
     9541 ctatagatga aaaaatagac ccaattggag cttgtgttgg accaaaagga cttagagtaa
     9601 aaaatatagt ggatgaactt ggcgatgaga aaatagatat aataaaatat agtgaagacc
     9661 cagcagagtt tatatcagca gcacttagcc catcaaaggt tgttagagta gatatagatg
     9721 aagatgaaaa gtctgcctta gtaatcgttc cagattatca gttatcgctt gctataggta
     9781 aagaaggtca aaatgcaaga ctagcagcaa aattgactaa ctggaaaata gatattaaga
     9841 gcgaatctca agcagaacaa gaaatgttaa atcttgaaaa ttccaaagaa atagaagctg
     9901 aggatgttga agctgaattt atcgaccaag aagagttaga tacttcagag gataatttta
     9961 gtgaagaaga gtaaggaggg atgattttga aaaaagtaaa aaaagtgcct caaagaaaat
    10021 gcatagcatg tcaagataga gactctaaaa aagagcttat aagaatagtt aaaaataaag
    10081 aaggaaaaat atttttagac ccaacaggaa gagccaatgg aagaggagca tatgtatgta
    10141 aaagctctga atgtctaaaa aaagctataa agagtaaagc attaaataaa gcttttaaaa
    10201 tagatgttcc tgatgaagtc tatgataatc tacttatgga gttagaagaa tatgaaaaat
    10261 aataaagaaa aagtactttc gttcttaggg ctcgcaactc gtgcagggaa aatagtgtct
    10321 ggtgatgatt caactttgct tgaccttaaa aaaggaaaag taaatttgat tttaatcgca
    10381 gaagatgcat caaataatac taaaaaactt tttaaagata aatcaacttt tagaaatatt
    10441 ccatatctat ttttttcaac aaaagaggaa ataggatttg caataggaaa atctccaaga
    10501 gcagttgtag gaataaaaga tgaaaatttt tctaaaaaga taattgaact aatagaaatt
    10561 taaaataaaa tagggggtga tcttgtgtca aaaacaagag tataccaaat agcagaagaa
    10621 ttaaatatat caaatgaaga gcttataaat aagttagctg aattagatat aaatgtaact
    10681 gacaaagatt cagtattaga aggtgaagag ttagaattag cacttgaaat gttaggtgaa
    10741 gatttatctc aagaaaatgg aaatgttata gaaatagatg gaaagttgac agtacaagta
    10801 ttagcaacga aattagataa atctccatca gaaataatta tgaagcttat gaaaatggga
    10861 actatggcaa caataaatca agagataagt tttgagattg ctgctttggc tgccaaagac
    10921 tatggatttg aattaacagt agctgaaagt gatgatacag aagctttaga aatagaagct
    10981 ttaatggaaa ttgaagaaga caaagaagag gatttaaaac ctagaccacc agttgttaca
    11041 gttatgggac atgttgacca tggtaagaca tctttactag atgcaataag aaaaactgat
    11101 gtgatctcag gtgaagctgg aggaataact cagcatatag gtgcttcaga agttaaaatc
    11161 aatggacaca aaatagtatt tttagatact ccaggacatg aggctttcac atcaatgaga
    11221 gcaagaggag cacaagttac agatatagca atattagtag ttgcagcaga tgatggtata
    11281 atgcctcaaa cagttgaagc tataaaccat gctaaagcag caggtgtacc tttaatagtt
    11341 gctattaata aaatagataa accaggtgca aacccagata aggttaaaca ggaattagca
    11401 gaccaaggat tattagttga agactggggt ggagaagtaa tagcagttcc agtatcagct
    11461 aagaaaaaag aaggaataga tacactttta gaaatggtat tattggttgc tgaaatggaa
    11521 gagttaagag ctaatccaaa taaaagagca gtaggaactg ttatagaagc tgagcttgat
    11581 aaaggaagag ggccagttgc aacagtactt gttcaaggtg gtactttaac agtaggtgac
    11641 ccaatcgttg caggtgttgc atgtggtaaa gttagagcaa tgataaatgc aaaaggtaag
    11701 agagtaaaaa cagcaggacc atctacagca gtcgagatat taggtctatc agaagttcca
    11761 caaggaggag accaatttgt cgaagttcca actgataaga ttgctagaag tgtagcagca
    11821 agaagacaac aaatagttag agatgaaatg ttaaaatcaa ctcaaagatt atcactagat
    11881 gctctattta gtcaaatgag tgaaggttct ataaaagacc ttaatatagt tataaaagca
    11941 gatgtacaag gttcagtaca ggcagttaaa caatctttag aaaaattatc aaatgaagaa
    12001 gttcaagtta aagtaataca tggtggtgtt ggagctgtaa cagaatcaga catattactt
    12061 gcagcagctt ctaacgcaat aataatagga ttcaatgtaa gaccagttcc aggtgcagaa
    12121 tctctaggag aaaaagaaaa tgttgatata agaacatata ctataatata caaggcaata
    12181 gaagatatac aagcggctat gactggtatg ttagaccctg aatatgttga tgaagaaaca
    12241 ggaaaagctg aaataagaga aatatataaa atatctggtg ttggaacagt agctggatgt
    12301 tatgtgacta atggtaagat atttagaaat tgtaaagtaa gattagttag agatagcata
    12361 ataatacatg aaggtgagct tgcagcactt aagagattta aagatgatgt aaaagaagtt
    12421 aattctggat acgaatgtgg tatgagtttt gtaaattata acgacataaa agaaggcgat
    12481 atagtagaag cttatataac taaagaagta gaaagaaaat tataaattcg tatcaaagaa
    12541 aatcagttag taaagaaggt cgatggtatg gcatcatata atagaacaag aaggatagca
    12601 gaagaaataa gaaaagtcgt aagtactatg cttataaatg gagtaaaaga ccctagaata
    12661 acatctatgg tgagtgtaac tgatgtagaa gttacaaatg acctaagata tgcttatgta
    12721 tatgtaagta ttctaggcgg agatgaagaa tccacactaa caggtcttaa aagtgctgga
    12781 ggatatataa gaagagaagt aggaaaaaat ataaaactta gatatattcc tgagatagta
    12841 tttaaacttg atgattcaat agaaaaagga atgtatatgg atagccttat aaaaagagtt
    12901 aatgaaaaaa atgctcaaca aaatgaggat gaaaattatg atgaataata tggatagtat
    12961 tataaactat atttatgagg gtgactattt tatagttacc tctcatatca gtccagatgg
    13021 agacaatatt ggttctacac tttcaatgta ttatacattg aagaatttag gaaaaaatgt
    13081 atattatgta cttgatgatg aagctccatt aaatttaagg tttttggttg aaggtgtaaa
    13141 aatctataag tctaatgaat ttaatatgaa gaactattct ctaattgcac ttgattgtgg
    13201 ggataagatg agaatatgtg tgagtgatga aatcaaaaat aatgcatcaa aattaatctg
    13261 tattgaccat catgctagta atgattcata tggagattta aattatgtag atattgatgc
    13321 atcttctaca tgtgaattag tttataattt attagttaga tttcagcaaa taaaagatat
    13381 taatattata aatgaagata tagcaacatg tttatataca ggtcttgtaa ctgatactgg
    13441 aaacttttcg tattcaaatg tacatgcaag tagctttgaa atggcaaaaa atttacttgt
    13501 tttaggtgca caaaaaaata ctataataca aaatatatat caaagcaatt cttctggtta
    13561 ttacaaatta ttaggagaag ctctaaaggg tttggagata tttgattaaa aaatagttac
    13621 gcacactcgt gactttagtc atgagttagt agctaaaata gtcagcatat agggaaactt
    13681 atatgtagag ataggataaa acctatccaa tactacttga attgctggaa acccctaaag
    13741 ctaaccaaac taaaacataa ggatgaaata aacctaagtg tgaaagttac gaaagtagaa
    13801 aaaattggtt agatggtata aggttaaacc ctaagtactt taaaatgggc aatcagcagg
    13861 ta
//
LOCUS       DAFKMX010000110        13828 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFKMX010000110 DAFKMX010000000
VERSION     DAFKMX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.110"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            141..1646
                     /locus_tag="LZB08_002485"
     CDS             141..1646
                     /locus_tag="LZB08_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBM7315980.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2036..3133
                     /gene="dinB"
                     /locus_tag="LZB08_002486"
     CDS             2036..3133
                     /gene="dinB"
                     /locus_tag="LZB08_002486"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBM7315981.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEFNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3396..4532
                     /locus_tag="LZB08_002487"
     CDS             3396..4532
                     /locus_tag="LZB08_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7315982.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4525..6072
                     /locus_tag="LZB08_002488"
     CDS             4525..6072
                     /locus_tag="LZB08_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBM7315983.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMFSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6406..7272
                     /locus_tag="LZB08_002489"
     CDS             6406..7272
                     /locus_tag="LZB08_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBM7315984.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7413..8555
                     /locus_tag="LZB08_002490"
     CDS             7413..8555
                     /locus_tag="LZB08_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBM7315985.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            8939..10177
                     /locus_tag="LZB08_002491"
     CDS             8939..10177
                     /locus_tag="LZB08_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7315986.1"
                     /translation="MDGDILMSKILKYKNEMLLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLILHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            10206..11093
                     /locus_tag="LZB08_002492"
     CDS             10206..11093
                     /locus_tag="LZB08_002492"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBM7315987.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            11127..11756
                     /locus_tag="LZB08_002493"
     CDS             11127..11756
                     /locus_tag="LZB08_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBM7315988.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            11761..12225
                     /locus_tag="LZB08_002494"
     CDS             11761..12225
                     /locus_tag="LZB08_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBM7315989.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            12475..>13828
                     /locus_tag="LZB08_002495"
     CDS             12475..>13828
                     /locus_tag="LZB08_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7315990.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     K"
BASE COUNT         5223 a         1458 c         2381 g         4766 t
ORIGIN      
        1 tcttgaaaaa aattaatctt aattgtataa tattactttg agtgaaaaag tattaacatt
       61 tttttataag atgttttact aaaaaaatat atataaatat actgttatca acaagaaatt
      121 taatttagtg aggtgttaag atgaaagtag gatttgacca caaaaaatat ttagaagagc
      181 agtctaaata tatcttagaa agagtaaata attttgacaa actttattta gagtttggag
      241 gaaaattgat gggagacctt catgcaaaaa gagtacttcc aggatttgat gaaaatgcaa
      301 agataaaagt attacaacat atgaaagaaa aagttgaagt tgtaatttgt gtctatgcag
      361 gagatataga aagaaacaaa ataagaggag actttggtat cacttatgat atggaagttt
      421 taagattaat agatgattta agaggttatg aactagatgt aaatagtgta gtaattacta
      481 gatttgaagg acaacctgct acaactgtat ttataaataa attagaacgt agaggaatta
      541 aagtttataa gcattatcca acaaagggat atccgtcaga tgttgagact atagttagtg
      601 atgagggata tggagcaaat gaatatatag aaactacaaa gccaatagta gtagtcacag
      661 caccaggacc taatagtgga aaacttggta cttgtctaag tcagttgtat catgaaaata
      721 aaagaggtaa tgtagttgga tattctaagt ttgagacatt cccagtttgg aatgtacctg
      781 taaaacatcc tttgaatata gcttatgaag cagctacagt tgatttaaaa gatgtgaata
      841 tgatagattc attccacctt gaaacgtatg gtgaaatgtc agtaaactac aatagagatt
      901 tggaattatt tccagtgctt aaaaaaataa tagaaaagat aactggaaaa gaatcaattt
      961 tccaatctcc aactgatatg ggtgttaata gagttggctt tggaataata gatgatgaag
     1021 taataagaaa agcttctata gaagaaataa taagaagata ctttaaaact gcctgtgagt
     1081 ataaaaaggg acaagttgat aaaggtgctt atgatagaat gaaaatgatt atggaagagc
     1141 ttaacttaaa gccagaagat agaaatgttg tattacctgc tagaaactac agtaataaat
     1201 tgaaagaaag tgccgacaag aatgatactt gtcctgtagt tgcattagaa ttagaagatg
     1261 gtacaatatt aactggtaag ggttctgatt taatgaatgg aactgcagca gtagtattaa
     1321 atgccattaa acatttagcc aatatatctg atgatatgca cttaatatca cctgtaatat
     1381 tagaacctat cataaactta aaaacaaaga ctttagctag tagaaatgtt tcacttagtt
     1441 gccaagaggt gttaactgca cttagtattt gtgctgtaac taatccaact gcacaatttg
     1501 ctatggaaaa gttagcatta ttaaaagggt gtcaagctca ttctactact atattaaata
     1561 gagatgatga acaaacattt agaaaacttg gtatagatgt aacttgtgac ccagaatatc
     1621 cttcacaaaa tctttactat agttaaaatt atataaaaaa atatgggctg ttttaaagta
     1681 atagtttgag cagtccatta caattataat aaaatcagta ggctgtcttg tataagacag
     1741 tcttttgttt ttaaaacatc ccataaatta aatatatata ctgttatcca taaggaattt
     1801 taaatttagt tccttggaat acctattgta aagcttttat atagtttttg caagtgtttt
     1861 taaggatttt ttataaatta tattgaagtt aatggattat agatggtttt atttatggaa
     1921 aatatgaata tataaagaaa ataatgaaaa aataccaaaa agaaacataa gtttggtata
     1981 attaaaaagt aatataaata aaattaatct atttatactt taaaaggtga taataatgaa
     2041 acattataga aaaataattc atatagatat ggacgctttt tatgcatcta tagaacaaag
     2101 agataataaa aaattaaaag gaagacctgt tattgtcggt ggaaatccac aaagccgagg
     2161 tgtggttgca acgtgttctt atgaagctag aaaatttggt atccattcag ctatgccatc
     2221 agctgtagct tacaataggt gtccatatgc agtttttgta agacctagat ttgatgttta
     2281 taagagtgta tcagagcaaa taagagatat attttataga tacacggatt tagttgaacc
     2341 attatcttta gatgaagctt acttagatgt aacaaaaaat aaaaaaaata ttgattcatc
     2401 aattgagata gcaaaacaaa taaaaaaaga tatatttaga gaagttggtc ttacttcttc
     2461 agctggtgtg tcatacaata agtttcttgc taagattgca tcagacttaa gaaagccaaa
     2521 tgggttaact gtaataaccg aagaaaatgc ccaggacttt ttagataaat tacctgtgaa
     2581 taaatttttt ggtgttggaa aagttacttc aaatactctt aaaaatttag gtataaaaac
     2641 tggatatgat ttaaggtgtt taaatttatt tgaacttgaa aatatattca aaaaaagagg
     2701 ttatgaatta tataaatttg ctagaggaat agacgataga ccagtggaac caaatagggt
     2761 tagaaaatct gttggggctg agactacatt aagtcataat ttggatattg atgaagaaga
     2821 aacacgcaac attcttgatg aactttgtga agaagtatgt catagattaa aaaactctga
     2881 gaagtttgga aaaacattaa cattaaaaat taaatatgag gactttacaa agataactag
     2941 aagccttagt ttagaacact atattgacga atataatgat ataagaagtg gtgtagataa
     3001 tttgttgaga aatgttgagt ttaatggaaa acaaattagg ctattgggag ttacaatatc
     3061 aaacctaagt gataaaaaag aaacttataa agatattaca ttatttgaat acatggatag
     3121 tatccaaatg tgagttttgt ttaaatgaaa taacactagc tttaatacta tataattgag
     3181 gctagtgtta ttttatgaat atttttatat tttttgtttt ttgacattat atattatata
     3241 ctatatataa atactttata tttgtagcaa tattcagaca aatagtgacc taaaaatcgt
     3301 actttgttta cataaataaa atagtatgat ataattataa aaacaactta taatattgaa
     3361 gaaggttgct tgttcgaaaa aggggatgat aaattatgat agagataaga aatgtaacaa
     3421 aaaaaatagg aaataatgtt atattagatg atatttctct agttgtagaa acaggtacat
     3481 tggtagtttt gattggttca agtggttgtg gaaagactac tactttgaag cttataaaca
     3541 aattaataaa acctacatct ggtgagatat atattaatgg caaaccaatt tctcaggaaa
     3601 atgaaataga gcttagaaga aaaataggat atgttataca gaacacaggc ctattccctc
     3661 atcttacaat aaaagaaaat atagagctaa ttccacgcct aaaaaaggaa aaatccgttg
     3721 aagaaattga aaaaagaacg ttgcaactac ttgagatggt aggacttgat agtgatgaat
     3781 ttttgaataa gtatccttca gaacttagtg gtggacagca acaaagaatc ggtgttgcaa
     3841 gagccattgc tactgatgct gaaataatat tgatggatga gccttttagt gctttagacc
     3901 caataacgag aacatcttta caagaacaat tattttctct tcaagatgaa ttgaaaaaaa
     3961 ctataatatt tgtaacgcat gatatggatg aggctttaaa gatagctgat aaaatttgta
     4021 ttatgaagga tggaagaata gcacaatatg atactcccga aaatatactt agaaagcctg
     4081 caaatgactt tgtaaaagat tttatcggag aagatagagt ttgggataat ccagaatata
     4141 taaaagcaag agatattatg ataaaaaatc ctattgctgt taattctact agaactgtaa
     4201 ctcaaggaat agaaattatg agaacgagta aggttgatag cttactaatt atagatagag
     4261 caaaaacatt aaaaggtatt gttactgtaa aagatatgaa agatattgat gataagtcaa
     4321 tattacttgc tgatattatg tcaagtgagc ctttgcatgt aaatgaaggt gataatttag
     4381 tagaaatact aaatgtaatg aatcgtaatt cggttggata tattccggtt atttctgatg
     4441 aaaataaact ggtagggctt attactagaa gtagtttact ttctgtttta agtgaacaat
     4501 tcttagaaat ggaggtgagt gttcttggct aattttttta actttatact attacaaaaa
     4561 gataagatta tagagctttt aatacaacat atgagtctaa ctgttacatc tattttaata
     4621 gctataatag ttggtgttcc tcttggaata attatttcaa gaatctcatc tcttagaaag
     4681 tttgtacttg gatttgtaaa cttagtccaa gcagttccgt ctatggcact tttgggtctt
     4741 cttgtaccta tacttggtat aggaagtaaa ccagccatat ttatggtagt ggtttattca
     4801 ttactaccaa ttgtaaaaaa tacgtatata ggtataacaa gtattgaccc agtagtatta
     4861 gaatcagcta aaggtattgg tcttacaaga aatcagacat tatttaaaat acaattccct
     4921 ttagcacttc ctataataat gggtggagtt agaatatctg cagttacagc tgttggtttg
     4981 atgactcttg cagcatttat aggtgctggt ggtttaggat atttagtatt ttcaggggtt
     5041 caaactgtaa ataataatat gattttagca ggggcaatcc catcatgtat aattgcactg
     5101 ttagttgatt atttatttag taaaattgaa gttgcagtta ctccaaaagg attaaatcca
     5161 aaagcaccta agaagaatta tatagcactt aaagtaatat ctgtagtggt tgttgtttca
     5221 atgttgttcg ttgtttttag ttctagtttt tcaagtaaaa aagatacaat aactattggt
     5281 tctaaagatt atacagaaca acttatactt ggtaatgttt atgctgaatt agtagaaaaa
     5341 aatacgaatc ttaaagttaa gaagaattta aatctaggtg gttcatctgt tgcgtttaat
     5401 gcaattaaat ctggtgagtt agacatgtat gttgactata caggtacact tttggtaaat
     5461 gtaatgaaac atgctccgat taaagatgct gatgaagcat ataatgttgt aaaagatact
     5521 atggaaaaag aaaatcagtt aactttactt gaccctcttg gatttaataa tacttacaca
     5581 ttagctatga tgcctgaaac tgctgaaaag tatggaataa atactatttc tgatttgact
     5641 aaatatggta aagaatttac attctcacca actttggagt ttgaaaatag agaagatggt
     5701 cttgtagggc tttctagaga ttatggttta aaattcaaag atgtaaaagc aatgaatgga
     5761 agtttaagat atacagcttt agataataat gaatctcagg ttatagatgc atttttaact
     5821 gatggtctta ttaaaaagtt taatcttaag atattagagg atgataaaaa tttctttgta
     5881 ccatattatg cagcaccatt agtaagagaa gatactttga agaaatatcc tgagttagaa
     5941 aaagttatga atatgttttc aggaaaaata aatgaagaaa ctatgagaga acttaactat
     6001 caagtggatg aacttggtaa atcaccagaa gaagttgctc attcattctt agttaaagaa
     6061 ggcttagtat aaaaaattaa tagtttatat caaaaattta agggagtgtc tcaaaatgaa
     6121 ctttttagtt catgaggcac tcttttttgt atgaagtcaa atatatttta tcatttcaac
     6181 aataaaaaag aagattatct ttattttgag acagtatctt atttttatgt atacaaaaac
     6241 catacctacg atttttattt ataattaagt ttcaaataga atgaacaagt acaaccaatt
     6301 ttgaatataa atatagtaaa tgtgcttata tacgatttta ttgtctggtt gattgatatt
     6361 gatagatagc tttctttatt gaatcgaagg gagggggaat ttgttatgga taactataag
     6421 aaagtgaagc aagtattgtg gataatttta tttgctaatt ttgcagtagc attattaaaa
     6481 atcattattg gtaatcaaat aaaaagctat agtatgacag cagacggatt tcattctttg
     6541 tcagatggag catctaatat agtggggctt ataggtattt tctttgcatc taaaccaaaa
     6601 gataaaaatc atccttatgg acataaaaag tttgaaatta taaccagttt atttataagt
     6661 ggtatgttgt ttgttatagc aattaagata attcttagtg cagtgttaag aatagctaat
     6721 ccagttgtac ctgctataac tatagaaagt ttaattgctt taattattac actttttata
     6781 aatatttttg tatgcatgta tgaatataga attgggacta aactaaatag ttatgttttg
     6841 atttcggatt cattgcatac aagaagtgat atatttgtgt ctctaggtgt attggttact
     6901 ttagttggtg ttaagttagg atttccagta ataatagaat ctatagttcc tattattatt
     6961 tctgcattca taatttattc ggcttatgga atatttaggc catctattgg aattttggtc
     7021 gatagagtgg ctgtagatga ggattatata aaagaaattg tgtttgaatt taatgaggta
     7081 agggatgttc acaatatcag aagtagaggt agcaaaagtt ctatttatat tgacatgcat
     7141 gtaatggtag acccatttat aagtgtagag caatctcatg atttaacaca taaaattgag
     7201 aaacaaatac aagaagaaat taatgaaaat gctcaagtaa tagtccatat tgaacctttt
     7261 tatagttttt aacttagtta taaaatacaa gttataaaaa aatataattt ataaaaaaaa
     7321 tattatacca taaataattt ataaaaatta tttatggtat aatattttct ataaagaaat
     7381 taagtcttaa tatatctaag gaggaattta caatgagata cgttgaatat ggaaagacag
     7441 ggaaaatggt ttcagtagtt ggctatggag gattaaggtt tgatttagaa aaaagtaatc
     7501 aagaaaatgc tgatttagtc aaatatgcgt atgaaaaagg tattaattac tttgatacag
     7561 caccagggta ttgtgatgac agaagtgaag atatatttgg actagccttt agacaaatga
     7621 taaaggaagg taagaccgat ttttatgtat caactaaaga aaagccaaaa gcatgtgata
     7681 catcagaaaa agctatagag gctgtaaaaa aatcaataga acgattaggc gtatctaaaa
     7741 taaattttta tcatgtttgg aatattagaa agatggaaca ttacgaacta gcaatgagac
     7801 ctggaggtca atatgaagga ttattgaaat gtaaagaaga aggattgata gaccatatag
     7861 tattttcatc tcatcaacca ggagatgagg ttatagatat tctaagtgaa aataagtttg
     7921 aaggtgtaac tatgggaata aatatattaa actttccata taggctaaaa ggtgccaaat
     7981 atgctgttga taatggatat ggtgttgtgg caatgaatcc attaagtggt ggaactattc
     8041 caaagtataa taaagaactt tcttttctat caaaagatgg cgaaagtact atagaatcag
     8101 ctttaagatt caacatagga atttcacaaa taagtatatc tttgataggg tttaataaaa
     8161 aacaagatat agatgatgca tgtaaaattg cagacgaaaa tagaatttat tcagatgagg
     8221 atatacatga tattgagact agattaaata agaatatgaa cgaaatatgt acaggttgtg
     8281 gttattgtaa agtatgtcca aaaggtatta ataccccagc ctatatgctt ttttataatg
     8341 aaaagcagat gtttaaaaag agtgatgaag aaatgacaaa gttagtttat ggcttaggac
     8401 attggaatta cacaatgaat agtaaggcaa aagctaaaga atgtatatct tgtggaaaat
     8461 gtgaagttga atgtactcag catttaccta taatagatag attaaaagaa attaaaaaat
     8521 gggaagaaga tggagctaat agtgttaaag tctaattaat tttatactat atttgaaatt
     8581 tgtatagtat ttagtaatgt tttaaaatat agttttaata tttaaggaga cagattataa
     8641 ttttgtctcc ttaaattatg ctttaatttg ttatagttta attccataag caacattgta
     8701 tatttttata taaagttttt caaatatttt atattaatat tgcttacaaa ttctcatttt
     8761 atacaacact aatctatgtt atataaacaa tacttgactg attataacat caataaagta
     8821 aatattgtgg ttgtaaataa agagttattc aattacaata aaacaagtaa gaacattatg
     8881 aagatggcta ttaaatataa ggaataaatt gtaggtgtca gcaaatccta tataatgaat
     8941 ggatggtgat atattgatga gtaaaatatt aaaatataaa aacgagatgc ttttgtttat
     9001 attagttgta ataacttact taataataac taagatattt ttttctaaga ctactatatt
     9061 ttatgattta aacaatactt atgatgtatt gttagataca gatacaggag ttttattcaa
     9121 tttaaatgta tttgctataa gtcaagacaa tagtaagcac atattgtttt cagcaattat
     9181 atcaattttt gcatatccaa tatacctatt ttgtacttca atagcaaatc ctgggacaac
     9241 agattttaat agtgcctatg ggtttggact aatttgtcta caaataataa ctagtgctat
     9301 gtcaataact ttagtcttta atcatattaa aaagataaaa atgcagagat taactttaat
     9361 tttacttact atgataatga tattttcttt tccacagtta tttatgactt taaatgtaga
     9421 gagatttata tatagccaat tttcattaat atttttcata gttattgcaa ataagatgaa
     9481 aggaaaaaat tcttatttag tagaattagc agctattcct ctatttggaa taactattag
     9541 taatatatat ttgtattttt ttaatatgat atttgagttt aaattaaaaa taggaaaaat
     9601 gttaaaacat ttaattacat ttatcctaat ggcatatatt tgtgtagttt caacgaagtc
     9661 ttatgagagt tttatgaacc ttggaaatgt aatacagtat gatacgaaat ttataagtgg
     9721 agaaccaatt ttggaaaaga ttgcaatgat tatagagaga ttattgtatt cggtttttta
     9781 ttttcctgga gcaagtatta aaaaagggtt atttttgcaa aatggagaag ttgctacaat
     9841 accagtaatt cttacattat tagctttatg tttttgtgtt ttatcagtta ttgaaaattc
     9901 agaaaaaaga gttcctaagc tatgtatggg aataattata ttcaatctaa ttttacatgg
     9961 tatagtagga tataatctag tcaattcaag tattatggct ataaatttta gttttgcagt
    10021 tataatttta ttggcttact ttacgaaagc gcttagaaaa aatgaaaaga atatgtataa
    10081 tatatttcta tcgctgttat tggttactat agtaatcagt aatgtcaatg gatttataga
    10141 aattttaaac atagggataa aatcatatcc tgtttaaaaa ataatattga aagggtagta
    10201 agtttatgaa acagaccaat acaagtataa ggcttcttac taaaatttca gattatataa
    10261 agttaatgag aataaagcaa tatataaaga atttctttgt attttcagct ataatttttt
    10321 caaacaacat actaaatata aatttatttt taaatacgtt tattgcgttt gtgtgttttt
    10381 gcctaatgtc atcatcagta tatgcactga atgatgctat agacatggat aaagataaaa
    10441 aacatcctaa aaaatgtaat agaccagtgg caagtggaag aataagcaaa aaaagtgcta
    10501 atatactatt tgtagtatta gctttaattt cagtctgttt atcaacagtt gttagtttta
    10561 atctttctat aattctcagt ctctatttga taaacaatat attatattct cttaaactaa
    10621 agaatataat actattagat gtgttttcaa tatctcttgg ttttatactt agggtttatg
    10681 caggatgtgt agccataagt gttagtttat caaattggat aattttatgt acatttttct
    10741 tatctctgta cttagctctt ggaaaacgta aaaaagaaat agaaactcta agagatgatg
    10801 cagttgaaca tagaaaaata ttggaggatt atgacataga aaatctaaat caaatgatga
    10861 tagttatatt aagttctaca attgtatgtt atgcattgta ttctacaagt aatccagaaa
    10921 agccacatat gatatttaca acaatatttg tagtttatgg tgtacttaga tataattata
    10981 taattaacac tacaaatgaa aataatccta ctgatattgt cctaaaagat aatgctttaa
    11041 aaataaacgt aatcttatgg ataattacat gtttaataat attgattttt tagtgatatt
    11101 tttagtgata ggatggggaa atacttatga aaaaaagtgt attattactt gatgtagatt
    11161 atactgttat aaatactgat agcatgatag atttttttat ttactcttta aaaaataaaa
    11221 cctttaaaac tattataaag ttgccataca ttatttttat tctatttatg tatttaatta
    11281 gaatgatacc cttgaagaag gcaaaagagg caatctttta tccaatagta gattttagtg
    11341 aagaagattt aaaaaagttt tttgatgatt gcataatgaa aaaaattaac gaatctatga
    11401 aaaaggttat ttacaaaaat aaagaagaag ataatgttat aattatgata actgcatctc
    11461 catatgcata tatgaaatat tttaagtatt atggtcttgc agacgaggtt ataggcacgg
    11521 agttttttta tgaaaattca agatataaaa atcaattcat tggagaaaac tgtaagggaa
    11581 ttgaaaaagt aaaaagaata aaggctgtat taggtaaact tgggatagag attgattatg
    11641 aaaattcata tgcatattca gactctaaga gtgatttacc aatgctttca ttgactaaaa
    11701 atgcattttt agttagtaaa aaggatggtg aggttataga gcaaataaat tcataactta
    11761 atgtcactta gaataggagg gaatagtttg ggatatttac acatattaat tttatctatg
    11821 gttcctgtaa cagaattgag gggagccatt cctataggta ttgcaatggg cttaaatcct
    11881 atatgggttt atgtatttag tgtaataggt tctacattgg tatcgattcc cttgatattg
    11941 acattcagac atgtattaca atttttaaga gggaagaaac tatttaaagg gattgctgat
    12001 gtaattgata gaaaaataaa ctcaagaatg aagaaattaa aaagtgtttc gataattggt
    12061 ataatactat ttgtaggtat accactgcca actacaggaa catggactgc atctgctata
    12121 gcttccatat taaaaatgag aataaaagat gcttttatgg gagtatttct tggcaatttg
    12181 ttatcagggg ttatagtttc agctctttct ttacatataa tataaaacta tctaagtaat
    12241 atacttggat agttttttta tgagtatgtg tgaaaaattt ttaataataa ataaaaaatg
    12301 agataaagta tttaacttca gttagttcta aaatgtttac tatggcaaaa gaaatatagt
    12361 atagtattac ttgtagggaa agtactctaa atttgtctgc taataaaatg aaatttttat
    12421 atagaataaa attttagtga tacattttaa ttattggatt gaggaggttt taaaatggaa
    12481 aattcaaaca ctaacttagg tagtgaaagt gtgggtaaat tattatttaa acttgctaca
    12541 cctgctatta tcgcacaaat tgtaaatgtt ttatacaaca tagtcgatag gatttttatt
    12601 ggtagaatgg aaaatggaga agttgctatg gctggtgttg gagtggcatt tccaataata
    12661 attataataa ctgcatgtag ttatttaatc ggtatgggag gaggtccatt agctgctata
    12721 aaaatgggtg agcaaaacaa tgatgaggca gagaaaataa tgagtaacag tttttctgta
    12781 ctggtgattt tagcaatact tttgactata ggttttaaga ttggaaaaga gcctttactt
    12841 tggatgtttg gagcaagtga atctacaatt ggatattcaa tggactattt aaatatttat
    12901 ttaataggta cagtgtttgt tcagatttca atgggtatga atacttttat aaatacccaa
    12961 gggtttgcta caactggaat gatgacagtt gctataggtg ctttaataaa cataatactt
    13021 gacccgattt ttatatttgg ctttaatatg ggtgtaaaag gagctgcatt agctactata
    13081 attgcacaag gcgtttctgc tatttgggta cttatgtttc tctttggaaa gaaaagtata
    13141 cttaaaataa aaaagaagta tatgatacca aaagcaagta tcatcttacc agtgcttggc
    13201 ttgggaatct ctccatttat aatgcaatct acagaaagtt tagtacttat agccttaaat
    13261 agtaaattac aaatgtatgg aggagattta gcagtaggtt caatggccat aatgagcagt
    13321 ataatgcaaa ttcttatgct tcctaatatg ggggttacac aaggagcaca accaataata
    13381 agttataatt atggttcagg tcaacttgat agagtaaaaa agacatttaa gttgtgttta
    13441 ttgtcatgtt ttacatattc tactatttta tggcttttat taatgatatt tcctgcattt
    13501 tttgttagta tatttaataa gaatccgcag ttacttagta tgactagttg gagtataaaa
    13561 atatattttg ctggtgcttt tatgtttggg attcaaatag cttgtcaaca gacattttta
    13621 gcacttggaa aagcaacaat atctttggtt ttagctctac ttagaaagat tgtactttta
    13681 atacctttaa tatttatact cccaacattc tttaatgaaa aactttttgc agtaatttta
    13741 gcagagccag ttgcagatat aacagctgct actataacag caatttcttt ctttatattt
    13801 tataaatgtt ttctatcaaa acctaaag
//
LOCUS       DAFKMX010000111        13334 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFKMX010000111 DAFKMX010000000
VERSION     DAFKMX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.111"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            171..2030
                     /locus_tag="LZB08_002496"
     CDS             171..2030
                     /locus_tag="LZB08_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7315991.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2322..2921)
                     /locus_tag="LZB08_002497"
     CDS             complement(2322..2921)
                     /locus_tag="LZB08_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7315992.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYTMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3024..3821)
                     /locus_tag="LZB08_002498"
     CDS             complement(3024..3821)
                     /locus_tag="LZB08_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7315993.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3934..4039)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4131..5117)
                     /locus_tag="LZB08_002499"
     CDS             complement(4131..5117)
                     /locus_tag="LZB08_002499"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBM7315994.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5601..7331
                     /locus_tag="LZB08_002500"
     CDS             5601..7331
                     /locus_tag="LZB08_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBM7315995.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7441..7521
                     /locus_tag="LZB08_002501"
     tRNA            7441..7521
                     /locus_tag="LZB08_002501"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7475..7477,aa:Leu,seq:tag)
     gene            7856..8371
                     /locus_tag="LZB08_002502"
     CDS             7856..8371
                     /locus_tag="LZB08_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBM7315996.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8863..8967
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9177..11651
                     /gene="gcvPA"
                     /locus_tag="LZB08_002503"
     CDS             9177..11651
                     /gene="gcvPA"
                     /locus_tag="LZB08_002503"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBM7315997.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11651..13108
                     /gene="gcvPB"
                     /locus_tag="LZB08_002504"
     CDS             11651..13108
                     /gene="gcvPB"
                     /locus_tag="LZB08_002504"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBM7315998.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDRYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4996 a         1581 c         2202 g         4555 t
ORIGIN      
        1 aaaaaaattt ttgtatatat agaggactct aaagatgaac tatgtaaaac aaggtttata
       61 tattctaata gtatagaaaa atttattgta aacgcatgta tattattaga aaaaatagtt
      121 tgaaggtatt caataaacat aaaaaacaat ataggggata aaggtgtttt atgaagagat
      181 ttttgaggag aattatctta gttttattta ttttgttgat ttttataagt tttatttcta
      241 ttaaattaat tcataatgtt ggagattatg gaaaattgat taattatgtt gggattgttc
      301 gtggagctag ccaacgtcta actaaactag agatgaatca tcaaccaaat gatgaattaa
      361 tagaatatat tgatgaaatc ctacaggagt tgattacagg gcatggagat tatggtttag
      421 taatcactga ttgtaatgaa tacaatgagg acttactttt attagaaaaa aagtgggaag
      481 atttaaatat tgaaattaaa aaagtaagaa tgaaagaaca gaataaccag ttattaagta
      541 taagtgaaga gtttttttct cttgcaaatg atacagtgtt taaaatagaa aacttttcta
      601 aagaaaaaag taattactta atgactttaa taataattat atctattata ggtatattag
      661 catgtatcat attgatttta caatatagta aaaaaatggt tagactggaa aaattaaatg
      721 tagatttaaa aaatattgca tataaagatg aactaacagg agtaaatact atagaaaaat
      781 ttaaattgga tgcaaatcaa aatatttgta tacataagga taagaaattt gctgtattct
      841 atattgattt tgaaaacttt aaatacatta atgatatatt tggatatgat tatggtgaca
      901 tgatactgaa aagatatgct aatcttatga tgaatgacat agggaaatat gaaatatttg
      961 caagagaaat tgcagataga tttgttgcgt taagatgcta tgtagataag gaagatttag
     1021 tggtaagaca acgagatgta gacaatgaac taattaatat aacaaatgaa attaaaaata
     1081 aacatagtat tactgttgtt agtggaattt gttgtattga agatgtaaat gaaaaattaa
     1141 gtatagatgg tttaataaat agagcaaatt ttgcacaaaa aactgttaag aataaaccag
     1201 gtactaatta tgctttttat aatgacagta ttcgtaaaaa aatgattgaa gaaaatacta
     1261 tcaagagtag aatacatgaa gctattgaaa aaagagaatt tatagtttat ttacagccta
     1321 aagttaattt acatagtcaa aaaataaatt gtgcagaagc tttagttcgt tggcttactc
     1381 cggataaagg tattatatcg ccagcaatat ttattccagt acttgagaag aattttttta
     1441 ttgccttagt agataagtat gtttttgagg aggtttgtaa atggattaga aagaggttag
     1501 atgaaaataa accttttgtt cagatatcag ttaatgtttc aagaatacaa ttttataaca
     1561 ctaaatttgt agaaacttat tccaatatac aaaataaata caggatacct aaaaatacta
     1621 tagaaattga atttacagag agtgttgctt ttgagaatca aaaacatctt ttggaaatta
     1681 tacatgattt gcatgaaaat ggatttactt gttctttaga tgattttgga aaaggttatt
     1741 cgtctttaag tgttttaaaa gatttaccat ttgatgtttt aaagcttgat tctatgtttt
     1801 tcaaggctag tttagataaa gataaagaga aaatagtaat aaaaaatatt gtacatatgt
     1861 taaaagaact taatatcaca actgttgctg agggcattga atgcaaggaa caagttgaat
     1921 ttcttaggga tattgggtgt gatttagttc agggatttgt attttataaa ccaatgccaa
     1981 tattagaatt tgaaaaaata ttggataaag agtttgttta taatagctaa aatgtacttt
     2041 ataaaatatg aactaattaa gtaaaattat tttgtactga ataaattttt actttattta
     2101 cagaataatg attatttaca gaaaagcgcg aaaagtgtat tcctttaagt gtgggatgga
     2161 tagcacaaat aggggagaaa aaatattggc catataagaa tgcataataa tataaatatt
     2221 tgaaaggtca gcattatata aaaattaaaa agaaaataaa taatttttct ataaaaaaga
     2281 gactatgaga aaagttactc atagtctcct aaataatgta tttattttct tcctgcaacc
     2341 cattttaatc cccaatgaaa ttcagtatcc atttctctat gacctttgaa gtatttaact
     2401 actttttcaa cactgaatgt ctcatcattt acattttcta tcaatgctat tgaacacata
     2461 gtatagccat ttttatgttc atcaagtttg acaactaagt ctgaatcttg tttttgtttg
     2521 attgttacaa ttccatcagc ttcagaccag ttagctacac cttcgtatat aaaagcataa
     2581 ataagtattc tttttatatc ttttatttta ttaccattta ttcttaaatt ttctccttgt
     2641 gtattacttc ctgttctgtc atctccatct aattgagcaa aaggtggatt gtctaaacta
     2701 ccaaaggcat ttcctaatgc ttgaacagca ccttttacac catttttcat ctcaaatagg
     2761 caacccaaat ctaaatctat attactgctt cttaaagagc caaaaaaacc ctttttttgt
     2821 actttttgat tccaatttaa attaactaag atttcaccta aatcgctatt ttcctttttt
     2881 gtaaggttta ttttttgtcc cttttttaat tctatagcca tagtaatctt cctccacaaa
     2941 catagattta tttaaatggt agcacttaat aagtcaatat tcaacaagat tagaataata
     3001 atattaaaat tatactaaat atactaaaaa gtaggaatca caataccttt gtatttttct
     3061 tcaattaagc ttcttgcttt atctgatgta agaacatctt gtagaacttt aatcttttca
     3121 ctgtctttat cttcagttct aaatgctaaa agatttgcat actcgtcatt tataggagct
     3181 gtataaattc cgtttttctt ggcatcaaga ccagcaggta cagcataagt agtatctata
     3241 aatgcagcag tcacatctgg aagagaagat ggaatttgag caatatctaa ctctttgaat
     3301 tgtattccct ttggattttc tattatgtcc ttaggactaa ctaatccatc ccctttaggt
     3361 aacgttaaaa gtccaatact ttcaaggtat ctaaggttct tagaacaaga agatgggtta
     3421 ttgcttattg caattgtatc tcctttttta aattcatcaa cagattttat cttataagag
     3481 taaagtattg ctgggcatac atatagtttt ttaccagcca ttattttgta acctttggcc
     3541 ttaacagctt cttttagata aggttcatgt tgaaatagat ttgcatcaat ttcaccatta
     3601 tttaaagctt cgtttggaag tatgtaatca tcaaagtttt taacttctaa agtatatcct
     3661 ttttctttaa taagtggttt taattcttct aataactctc ctcctggaac taaagtagca
     3721 ccaacaacaa tttttttatc atctttagct ttagaacatc caaccacagc taaagttaat
     3781 actagacata ataacaaaca taataatttt ttgaatttca tataaattcc ctcctgaata
     3841 taattgatta gttaaaaata aaataccatt ctttatcaca ctgcaaaata aaaataagcc
     3901 tgagtttaaa ctcaggcttt aaaagctaat ttagactcat ctttcagtat aatactgcag
     3961 gatttagcac caatgtatat aaaaatatac tggttgctgg gtttcatagg gccagtccct
     4021 tcaccactct ggataagaag tatcaatttt taattatgtt ataaatatta tcatgatttt
     4081 ttacgtctgt aaacataatt ttataaatat tttaaaatct ttaatgtatt ttatttaaca
     4141 cctaaaattc cagaaactag aatatcagta tcagcaccta agaaatagtc atttatttca
     4201 atagaatcca gagtatttct caaagcattt tcttcatgcc ttataccaat aagtttattt
     4261 tctacaaatg caacatcaca ttttccaaaa aaatctccaa aaaattttat actagttatt
     4321 atgcctttat caacatttaa attaaactca acatttccac cagaatattt taattcattt
     4381 gaaagagtat attttggtga attaccaaaa ttccaatccc aagtttcgta ttttgattta
     4441 gtaagctttt ctatttgttt tatatcatgc tcactcaaac aataattagt attatcagta
     4501 tttgttttag aaagataatc cattatagca tctttaaatt ctaatatatc catttttgaa
     4561 tgtaaatgtt cacttatatt ggttacacga gattttacag attttattcc ttttccttca
     4621 aattttatag gtttaacctt taatgctttt gagaggtcat ttatttcaga tgaaaataaa
     4681 agagtcccat gatgcattac tttgtttttg tagttgtatt gagcatttcc agaaaatttc
     4741 ttgccatcta taagaaggtc atttcttcca gaaaattcgg cattgacatc tagtgtttta
     4801 agtaaatcta ttatgggctg agtgaatctt ctgaaatcac taaaattatt attgttacaa
     4861 gctataaatg taaaatttat atttcccaaa tcatgaaata cagctccacc accagactgt
     4921 ctcctaacga caggtataga atattctttt atatattcta aatttatttc agaaagtgtg
     4981 ttttggtttt ttccaacaat tatagaaggt tcattcctcc ataaaataaa taagtcttca
     5041 tcaaaatttt ttagaagata ttcttccatt gctaaattaa aataagggtt tgttttttca
     5101 ttataaatta ataacattaa agtagttcct ccatagaatt gataaaattt ttattatgaa
     5161 tacaattaat tttataattc gacaataata taaaaactcc tttaaattag aaattttaaa
     5221 aactaccttg caaagtttaa ataaataata tatactcaaa atatgtttaa taaaagtgaa
     5281 tagaagggta gaggcgcaat aatctatcag tactaataag gagttaagca cagagagtta
     5341 ttaggaaagg agatattgcc gaagttttat aatcgatgct ttaagattat attgctgggg
     5401 gtacatagaa aatatgtaca actgtcacag gtttatttgt ggtgagctat catttaagga
     5461 attatattat atttattttt actattgttg atatattgta atgtttaagc cttgagtgtt
     5521 taccactcaa ggctttttta gttttattgt aacataatac cctgtacagt tcacaaaatt
     5581 aaaaggagag ggagatttat atgaggaaca aaaaaattaa tattattttt ctgacgacaa
     5641 tcatgttcat aatgtcaaca gtaatggttt ttgcagaaga agatatagac actattgcac
     5701 tagccaatgc agaaaaattt gggattttaa ctttaatacc accattagtt gctataatac
     5761 ttgcatttat aactaaaaac gtaataattt ctttacttat aggtattctt tctggtagtt
     5821 ttataattaa agctagtggt attaatgttt ttgcgacatt tatacaggca tttttagatt
     5881 tagtagacag agctttagta tctttagcag acccttggaa tgcagggata atactacagg
     5941 ttttggcaat tggaggagta ataaatcttg ttgctaaaat gggaggagct aaagctatag
     6001 ctgaagcact tgctaaaaga gcaaagtcag caaaagggac acagcttata acatggtttt
     6061 tggggctttt agtattcttt gatgattatg ccaattcact aatagtaggt cctatgatga
     6121 gacctgttgc agataaaatg aaaatatcga gagaaaagct ggcatttata atagatgcca
     6181 cagcagcacc agttgctggt ttagctataa tatctacatg gataggtctt gaggttggtt
     6241 taatacatga tgcttttgaa agtataagta tagatgtaga tgcatttggt atatttttaa
     6301 atacaatacc atttagattt tacaatatat taatattagc tttcattgta atatcagcat
     6361 tattattaaa agaatttggt ccaatgagaa aagctgagat aaaatctaga agtagaaaaa
     6421 ttagtataga ccttgatgaa ggcgtagaag agttggatga tttagctcca aaaaatggag
     6481 taaaattgag tgtatggaat gctataatcc caataggaac attgataata gttgcattag
     6541 catcatttta ttatagtgga tatacatcta taatgggagg agacgataag gctctgatac
     6601 aactatttac aaattctcca tattcttttg aagcaataaa agaagcattt agcgcatctg
     6661 atgcatctag agcattattc cagtctgcac ttgttgcaag tttagttgct attataatgg
     6721 ctgtagtaaa aaagattttt acaatatcag aagcaattga tgtttggata gatggaatga
     6781 aaagtttagt aatcactggt gtaatattaa tacttgcatg gtcattaagt tcagtaataa
     6841 aagagttagg tacagctaaa tttttaatac acctattatc aggttcacta ccaccattct
     6901 tattaccaag tttaatattt ggtttaggag caataatatc ttttgctact ggaacagctt
     6961 atggaacaat gggtatactt atgccacttg caattccatt agcatactca ttaaatcctg
     7021 atatgtctta tgtaatagta agtactagtg cagttttaac aggtgcaata tttggagacc
     7081 attgttctcc aatttcagat acaacaatac tttcttcaat gggagctggc tgtaatcata
     7141 tagaccatgt taacactcaa atgccatatg ctatatttac agcagtaatc actattgtat
     7201 ttgggtatat accagctgga cttggattgc caatatatat agtattacca gtggcaatag
     7261 ctgctatatt tgtaggtatt caaataattg gtaagaaagt tgatgaagcg gaaatagaac
     7321 ttgtagaata aagatataaa taagtttcac cttttattat cactattttg ttagttttta
     7381 gataatttta aaaagttatt gaaaataatg gcgaaatata gtaaaataaa agagtgataa
     7441 gcggatgtgg cggaattggc agacgcacta gacttaggat ctagcgccat tggcgtgggg
     7501 gttcgactcc cttcatccgc actttacagt acttaattgt actgaaaaaa actggggttg
     7561 ctggctttcg gagtgggcta tcccgttttt atttttttgt atatataata gattggcact
     7621 ttagtataag gtgctttttt tataggcaaa aacagtacat ttatttctgt tttattatca
     7681 ttcatctatt agaatatact ttagttaaga atgtgttaat aagcagtgtt cacattcttg
     7741 ataaaatgtg gaataatatg tatttttact aagacggaaa tgacataatt aaaccattaa
     7801 ataatattat aagatgatga ataagtagat aaatcaaatt ttgtaggagg atattatgga
     7861 aaaatatata tggttgtttc cattactttt tatttttcat gatatggaag agataatagg
     7921 ttttggaata tggcttaaaa aaaataaatc aatgcttgat aagaagtatc catttatcag
     7981 taaagtttat gaaaattata gtactgaagg catggcattt gctgtgtttg aagaatttat
     8041 tttatgcatt attttctgta tacttacagt tataacagag aatcagtatg tatatctact
     8101 atggttaggt agttttatag catatacttt acatttggta attcatatag ggcagtcaat
     8161 cataataaga aaatatatac cttcacttat taccagtatc atatgcttac caataagcat
     8221 ttggtgtata agtaagagta tttatatagt tgattgtgaa atgagtacaa caattctgta
     8281 cagtataatt ggcattataa ttgtagcatt gaatttaaag tttgcacagt cgctgattgg
     8341 aaaatttaca aagtggatgt caaacatata aattttagtt tattggacag ttcaaggaat
     8401 cagaaattgg aattttatag tattaatatt ttatggtatt aatataatgt ataaaatttt
     8461 atagcaatgt atttcataag tataaagtga ctagaatttt aagaaatata caaattctat
     8521 aaaaactaaa taatttcaag tatattttaa aaatcttatt tttcacaagt ttaaaattgt
     8581 aaacgatttc atcaagtcta tgaatatact tttaacatgt tatagtaaat ctcaatataa
     8641 atttacattt ggttctttgt gtgttatttt aataataaat taagtgaaaa tgttatttta
     8701 cattaatata taatatcttt gcataagagt gaataatata aaagagaaat aattgataat
     8761 aaaaacttaa caaaaaaatt aatacataaa aaaaaataaa tataatgtgt tgacatttaa
     8821 ttgaaatatt catacaataa aattgaaaat aaatatttcc aaatgagatt agcgagagag
     8881 tcctttatac gaaaaggcgc cgaagaagaa atttaagaaa tgccaatttt tagagaagct
     8941 ttcaggcaaa aatatcgcta attgatggga ctctgaagag acttaagtta agatactata
     9001 agtcaccgaa gaagcaaaat aatattaaat tattaaatct ttcaggtata caaacaggga
     9061 tatataggct aggattattt tccatgctga tatatctttt ttttatgaaa aagtacagga
     9121 aataatatca aagttactta aatacagtag gtctaatttt ctagggaggg ttatatatga
     9181 atgaattgaa aagagtatct ttgtacaata tacacaaaga gcttggagca aaacttgttg
     9241 aatttgctgg ttgggaaatg cctttagaat atgagggtat aaataaagag catgagaagg
     9301 tcagaaaaag tgcaggtata tttgatgtat ctcatatggg agaggtacag ataaagggag
     9361 cagagtcaga aaaatttata caaaacctag ttacaaatga tataagcgca ttaaagataa
     9421 atgatattat atatacacct atgtgttatg aaaatggtgg agtagttgat gatttattaa
     9481 tatataaatt tggtgaagaa gattatttat tagtaataaa tgcaggtaat atagataaag
     9541 atgtagcatg gataataaaa caaagtgaag gatacaatgt agatataaaa aatatatcta
     9601 gtgaagtgag tcaattagca attcaaggac ctaaagcaga agaaattctt caaaaaatca
     9661 cagatataga cttaaactca ataaaatttt ataagtcaat tccatcaaca aaagtatgtg
     9721 gttgtccttg tttagtttca agaacaggat atactgggga agatgggttt gagatatact
     9781 gtaaaaataa atatgtagaa ataatttgga atgaagttct aaaggttgga ggagaagata
     9841 tatgtccagc tggtttaggt tgtagggata ctttgagatt tgaagcagct ctacctctat
     9901 atggacatga aataaatgaa cacatatctc caattgaagg tggtttatct atctttgtga
     9961 aaacaaataa agaatcattt attggcaaat caattttatc aaaagaaaaa gaaagtggag
    10021 ctaaaagaaa gttagtagga tttgaaatgc aaggtaaagg aatgcctaga aatggatatg
    10081 atataaggat tggtgataaa acagttggat ttgtaacaac aggatgtgca tctccaacga
    10141 caggaaaaat tttaggtatg ggaattatag attctgaata tgcaaaagta ggaaatgaaa
    10201 taggtatagc aatcagaaaa aaagttgttc ctgctgtgat agttaagaaa cctttttata
    10261 aaaaacaata taaaaaagat aatattatat taaacaaaga aaataaattt tcatatatac
    10321 ctgctacaag tgaagataaa tcaaaaatgt taaaagtagt tggattaaat tcggttgatg
    10381 aattattttc agatatacca gaagaagtaa aattaaaaag agatttaaat ttggaaattg
    10441 gaaaatctga attagaggta agcaaaatag taaaaagatt gtcagaagaa aatttaagtt
    10501 tagaagactt gacttgtttt cttggtgctg gtgcatatga ccattatata ccatcaatta
    10561 taaaacatat aacttcaaga tctgaatttt atacagcata tactccatat caagctgaaa
    10621 taagtcaggg aactttacaa gtagtttttg aatttcaatc tatgattgca gaaattacag
    10681 gtatggaaat tgcaaatgca tcaatgtatg atggagcaac tgctgcaata gaagcatgta
    10741 taatggcaat gaatcagact agaaaaagta aaatagtagt gtcaaaaact attcatcctg
    10801 aaactttgtc aatattaaga acttacttac aatataaaga ttgtgagata gtagaaattg
    10861 acttttgtaa tgaatatggc actacagata tagaaaaatt aaaagcttct gtagacaaag
    10921 atacagcatg tgttcttata caaactccta atttctttgg aattattgaa gaaatggaag
    10981 aaatagaaaa gataactcat gaaaataagg ctatgttaat tatgagtgta gacccaatat
    11041 cattaggagt tttaaaaaca ccaggtgaga taggtgcaga tattgttgtt ggagaagctc
    11101 aatcacttgg aaaccctctt aattttggag gtccttatgt aggattttta gctagcaaat
    11161 ctaaatatac tagaaaaatg ccaggaagaa tagttgggca aagtttagat gtagaaggta
    11221 aaattgcata tgtattaact ttacaaacta gagaacagca tgtaagaaga gaaaaagcaa
    11281 cttctaatat atgttcaaat caagctttaa atgctcttgt agcttcaata tatatagcta
    11341 ctatgggtaa agaaggattt aaagaggttg gtatgcagtc tatgaaaaaa gcacattata
    11401 cttacaataa gcttgtacaa actggaaaat ataagccaat atttaaaggt aagtttttca
    11461 aagaatttgc tgtacaagga aatcttaata tagaaactat aaatgataaa ctcttagaag
    11521 aaaatatatt aggtggttat aatttagaat acaattatcc agaattaaaa aattcaactt
    11581 tgctttgtgt gactgagaaa agaagcaaag aggaaataga taagttagtt ggaataatgg
    11641 agggattata atgaaagaat acaatagttt gttgatagat atatctaaaa aaggtaggaa
    11701 agcatactca cttccaaagt tagatataga tgatataaaa atagaaaata tgattgacca
    11761 agatatggca agacaaagtg agttgaattt gccagaagtt ggtgaattag aattagttag
    11821 acattataca ttattatcaa ataaaaactt tggagttgat acaggttttt atcctttggg
    11881 ttcttgtact atgaaatata atcctaaaat aaatgaagat atggcagcac ttccaaactt
    11941 tactggaatg catccatacc aatcttcgga tacagctcaa ggttctcttt ctttgatgta
    12001 tgatttatca agaagacttg cagaaattac aggtatggat gaagttacac tacaaccttc
    12061 agcaggttct catggtgagt ttacagggct tatgattata aaagcatatc atgaaaatag
    12121 aggtgaccat aagagaacca aagtgataat tccagattct gcacatggaa caaatccagc
    12181 aagtgcagct atggcaaact ttgatgtgat tcaaatagca tctgataaaa atggagctgt
    12241 agacataaat gcattaaaag aggttttgaa tgatgaagta gcagctctta tgcttactaa
    12301 tccaagtact cttggtttat ttgaaaaaaa tataaaagaa atagctactc ttgtacatga
    12361 agcaggagga cttttgtact atgatggtgc aaacatgaac gctattatgg ggataacaag
    12421 acctggagat atggggtttg atgtggttca tcttaatctt cataagactt tttcaactcc
    12481 tcatggaggt ggaggtccag gagctggtcc tgttggagtt aaaaaagaat tagtaccttt
    12541 tttaccaata ccagtaattg aaagaaaaga agataggtat gtactaaatt atgatagaga
    12601 aaaatctatt gggaaaatta aaaatttcta tggtaatttt ggagttcttg taagagctta
    12661 cacatatata ctaactatgg gaagggatgg acttaaagag gctagtgaaa tggcagtttt
    12721 aaatgctaat tatataaagg aaagtataaa agatgattat attttgccaa tagatacctt
    12781 gtgtaagcat gagtttgtat taggtggatt gccaagagga gaggccaata taaaaactat
    12841 cgatattgca aaaagactac tagattatgg ataccatcct ccaacaatgt actttcctct
    12901 tattataaat gaggcactta tgatagaacc tacagaaagt gagagtatag agacattaga
    12961 tagttttata gaagctatga aaagtgttgc taaagaggca aaggaagaac cagaactatt
    13021 gaaaactgct ccacataata ctttggtcaa aagagtagat gatgcaaggg cagtaaagaa
    13081 acctatacta acatggtcac aaagataaat agttaaaaag tagagattta aagtataaag
    13141 agaattaaat ttgtaagaaa aagctatcta acttaatcta gtttagatag cttttttgta
    13201 tattagtatg tatggtattt tctatataaa gttttaaatt atattagtgc ttatatagaa
    13261 tattgatatg atgattatgt atagatatta ataagaattg ttgaatttat cttgccttaa
    13321 ctttatttac tctt
//
LOCUS       DAFKMX010000112        13235 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFKMX010000112 DAFKMX010000000
VERSION     DAFKMX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.112"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            9..710
                     /locus_tag="LZB08_002505"
     CDS             9..710
                     /locus_tag="LZB08_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBM7315999.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            981..1799
                     /gene="larE"
                     /locus_tag="LZB08_002506"
     CDS             981..1799
                     /gene="larE"
                     /locus_tag="LZB08_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBM7316000.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1854..3002
                     /locus_tag="LZB08_002507"
     CDS             1854..3002
                     /locus_tag="LZB08_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBM7316001.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3138..4526
                     /locus_tag="LZB08_002508"
     CDS             3138..4526
                     /locus_tag="LZB08_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7316002.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4555..5253
                     /locus_tag="LZB08_002509"
     CDS             4555..5253
                     /locus_tag="LZB08_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316003.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5256..6689
                     /locus_tag="LZB08_002510"
     CDS             5256..6689
                     /locus_tag="LZB08_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316004.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6711..7448
                     /gene="truA"
                     /locus_tag="LZB08_002511"
     CDS             6711..7448
                     /gene="truA"
                     /locus_tag="LZB08_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBM7316005.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7559..7766
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8019..9218
                     /locus_tag="LZB08_002512"
     CDS             8019..9218
                     /locus_tag="LZB08_002512"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBM7316006.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9465..10511
                     /locus_tag="LZB08_002513"
     CDS             9465..10511
                     /locus_tag="LZB08_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBM7316007.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10619..11782
                     /locus_tag="LZB08_002514"
     CDS             10619..11782
                     /locus_tag="LZB08_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7316008.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSVVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11871..12251)
                     /locus_tag="LZB08_002515"
     CDS             complement(11871..12251)
                     /locus_tag="LZB08_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBM7316009.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12254..12913)
                     /locus_tag="LZB08_002516"
     CDS             complement(12254..12913)
                     /locus_tag="LZB08_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBM7316010.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERVSIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5309 a         1311 c         2279 g         4336 t
ORIGIN      
        1 agataaatat gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga
       61 ttgatattga tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac
      121 caattatagt cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta
      181 aagagtatgg ttctcttgaa aactatagag agtattgttt aacaaaatat aatgaaattg
      241 atgtaaatca attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa
      301 ttgatttaga agatgatttt tatgattata aacattttag accaattatg aatatagata
      361 tatttcatga atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag
      421 tatttgcata catacctatt ggcggattta atgattgtcc tagagatgaa gaacatatag
      481 ctattgcaaa atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata
      541 cagttcaata ttctgtaaaa aatttaacta aagatggtaa aaaatttgat gaattatgta
      601 ttgaattagt attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc
      661 ttaaggatgt attaaaacgc tctacaatag tgcttttttg gtgggattga tattccatca
      721 tttagatagt attaaattgt tagtcaccaa gaatccactt gctttagtta tggggagtgt
      781 gtcaaactag gtttttatac acagtgtagc atagtaatat gtttaaatta caaaggttaa
      841 aaaaatactt taatattttt aatattataa ttaatagatt taaaggtaat tataatttat
      901 aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata tatagtttta
      961 tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa aaattagata
     1021 aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct ggtggagtag
     1081 atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt atagctgtga
     1141 ctattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa tatactcaaa
     1201 attttggtgt taaacatatc atattaaata tagaaaactt tgatttaaaa gaatttaaag
     1261 aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa ataaaagagg
     1321 tagcaaaaga acataatata aagtacatag tagatggaac aaatatagat gatttaggag
     1381 actatagacc tgggcttaag gcattgagtg aattaggagt aattagtcca ttaaaagata
     1441 gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaac attaggatta aatactttta
     1501 ataaaccatc atttgcatgt cttgcgagta ggatacctta tggagttgaa ataacagatg
     1561 aaaatttaag aatagtagaa aagagtgaag aatatctatc caatttaggt ttttctcaat
     1621 ttagagtgag aatgcatgga gatatagcta gaatagaagt tggacaggaa gaactaggta
     1681 aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata tttggcttta
     1741 aatatgttac actagatatg tcaggatata aaatgggaag tatgaattta aatgtgtaga
     1801 ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt tttatgaaaa
     1861 attatacgtt aatatcgcca tgtttttttg gaatggaaaa aatgctggct agagagatta
     1921 ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat aaaactgatg
     1981 agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta catcttaaaa
     2041 ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa agaataaact
     2101 ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc tctataaaat
     2161 caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata gtagaaagct
     2221 taaagaaaag ttatttggag gatggtttgc ttaaagaaga taaggagaaa taccctattt
     2281 ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga gatgctttgc
     2341 ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact ttagctgctg
     2401 ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct atgtgtggtt
     2461 ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct ggcttaaata
     2521 gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg gatgtaagaa
     2581 aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga tatgatatag
     2641 atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta gatgaatata
     2701 tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt ggatttataa
     2761 tcactaatcc tccttatgga gaaagattag aagataaaga cagtgttaaa cagttgtata
     2821 aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg ataacttctt
     2881 atgaagactt tgaatatgag tttgggcaaa aagcagataa aaaaagaaaa ttgtataatg
     2941 gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga aataataaat
     3001 aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta aaacattatg
     3061 atgccttgaa acgtctaatt attgtgagga gatttttgtg aacaaattta aagatttata
     3121 ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc ctgatgatgt
     3181 tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg aagatacctg
     3241 actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta aaaccagatt
     3301 catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag gtagatttaa
     3361 acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta agtaataatg
     3421 tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta cttggagacc
     3481 attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg gactcagatg
     3541 gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg catatatata
     3601 aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg cttcaaaata
     3661 aagaagattt taatgatatg aaagtaaaaa ttggatatat agatggagat gacaagttag
     3721 acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt gttgcaaatt
     3781 ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa aacttaagtg
     3841 aattatatat aacattaggt aatgttgctt catctgttgg aaattcttct gcagctataa
     3901 aaggtattgt acttgatatt cctatattga aagataataa ctatattacg cagatacttt
     3961 atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact attactaaat
     4021 cttattatat acctgtagat gatattgaca aagataagat tatagaaata cctatagttg
     4081 caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca actataagtt
     4141 ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca agtcagatat
     4201 attataatta taaatataat tttaaattat ttattccaaa taatttattt gataaaatac
     4261 ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat tttgactatt
     4321 cagaccgtaa gaatttattt aatatagtag tagagagtaa aaataaactt gaagatagaa
     4381 aaaatacagg tactcaaaat tctattgtac ttcaagagag cgatgaatat actttcttac
     4441 tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac gcattaaaag
     4501 aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta ttttatgaag
     4561 gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag tataaactta
     4621 aaaagggaag gctatgaaat agttgaggca ggtactggaa gagaagctat agaaaaaatg
     4681 acaacagaaa aggatattac aatagctctt ttagatgtaa tgcttccaga tataagtggt
     4741 atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat aatgcttact
     4801 gctaaagctc aagaagatga taaaatagaa ggatttatct caggagcaga tgattacatc
     4861 ataaaaccat ttagtataaa agagctttta gtcagagttt ctgctcttct tagaagagta
     4921 gcaaaggatg attcaagtgt taagtcgagt gagatagttt ctccaccatt tatacttgat
     4981 atagacaaaa gaaaattatt taaaaatgga aaggaaattg agcttacacc aactgaattt
     5041 tcaatagtga aatatctaat atcaaatgct aagcagtcgt taagtagaga ccagatatta
     5101 gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt aaatataaga
     5161 aggataagaa ataaaataga agatgaccca tcaaaaccaa aatatataca aactatttgg
     5221 ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa ggtctgagaa
     5281 aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg tttgaaggat
     5341 tgttcatgtt ttacatacaa aactactatt atgattctgt caaacagcta cttgaatctg
     5401 aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt tttgagaaaa
     5461 aagtaaaaaa tatatttgat aaacaaccct taactaaaaa ttcagaattt ggaatttcaa
     5521 taatagataa agataaaaat attatattag accaatatgg ttttaaaagt aaagaaaaag
     5581 caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat ttaacacctt
     5641 atacttatag aataccagat acaggggagc atgttatgag tatctcctta ccacttaagg
     5701 taaataatat aatagaaggt gttgttagat atacagtatc attagatgct attgataatg
     5761 caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt attatagcta
     5821 tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag ctaaagaaat
     5881 ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg aagattgtag
     5941 atgatgaaat tggtgattta gcacagacat ttgaacacat ggcacatgag attgacaaaa
     6001 gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg actcctctta
     6061 catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga gaagaactag
     6121 acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta gttgaagaat
     6181 tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat atagttgatg
     6241 ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct gaaaaagata
     6301 tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga gataaaaata
     6361 gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg agtcaaggtg
     6421 gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca gtagaggata
     6481 atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt ttccaagaag
     6541 attataataa ggctggtagt ggattaggac tagcaattag taatgaaatt gtaaaacttc
     6601 atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata acttttaata
     6661 taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag atgagaaaca
     6721 taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa ttaggtgaca
     6781 ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact aatgaaacag
     6841 tggagataat aggttctgga agaactgata tgggagctca tgcaagagga caagtggtaa
     6901 attttagaac taattgtatg gattctttgg ataaaattca aaaatattta tatgaatatt
     6961 taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt cattcaagat
     7021 acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat catagtcctt
     7081 ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga atgagaaaag
     7141 caagtgagta tttagtagga gaacatgatt ttaccagttt tgcttcttct aaatctaaga
     7201 aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat aatgttatag
     7261 aaatatatgt agaaggaaat ggatttttgt ataacatggt aaggataata gtaggtgctt
     7321 taatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg cttgaatcaa
     7381 aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt tggaaagtaa
     7441 catattaatc aattatataa aactctcttt aaaataaaaa taaaataaat tatatagttg
     7501 acataaatgg aaaaataaac tattatatat tttataagag agatataaaa ctttatattt
     7561 aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag gatggtggaa
     7621 gcttggcaaa taagtattag tgaaaagaac tttggaggat actaattgaa tttagtatgt
     7681 tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca attagagtgg
     7741 taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat atatttaatt
     7801 attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt tatcaataag
     7861 agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt ttttttagta
     7921 ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag aatttctata
     7981 aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa gtagtattag
     8041 catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa aattatgaag
     8101 atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg gaagatgtat
     8161 atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata agtgaagaat
     8221 ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa ggaaaatact
     8281 tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa gttgctcata
     8341 aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac caagttagat
     8401 ttgaagctac aattgcagct cttgacccga ctataaaagt tattgcacct tggagaattt
     8461 gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat ataaaggtaa
     8521 cagcaactaa agctaaaata tattcagtag atgctaacct ttggcatgtt tcaacagaag
     8581 gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa caatgtgtag
     8641 acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa aaaggtgtac
     8701 ctaaaaaggt aaatggtgaa gaattatctc cagttgcact gattcataag ttaaatgagt
     8761 tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt gttggtatga
     8821 aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca cataatattt
     8881 tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta tcttataaat
     8941 acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct atagatgcat
     9001 ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt tacaaaggaa
     9061 atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga atatcttctt
     9121 ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat ttattcactt
     9181 taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta tttaagagtg
     9241 tataaaaact atttttaggt ttttatatgc tcttttttta tttgaattta ttaacttata
     9301 cagtaaatag tataaaaaaa tatattatag ttccaaatgg cttaataatg tgaaaatata
     9361 agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt gtaagagggt
     9421 ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga aaaataattg
     9481 aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa gatttatata
     9541 taaataaaac cggtgaatgc cttccaaacg attttttctt tttactgtca ggttttggtt
     9601 cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct ttgggagatg
     9661 gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa tacaaacatc
     9721 atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt gatgatggat
     9781 atccagttat tttaggagct ttagatatgt attatctacc atattataag aagctatatc
     9841 ataaagaaca tattcctttt cattatatat taatggtagg atatgatgat gaaggaatat
     9901 atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa ttacgtgaaa
     9961 gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca ataagaatga
    10021 atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag cgtgatatgt
    10081 ttttaaatcc acctaaagga tttttgggat ataaaggttt tgaaaagttt attaaagagt
    10141 tgccacagtg gaacactgaa ttggataaag atgattatga taagattttg accaatatgg
    10201 ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt aaagaaccag
    10261 atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac agtattgggg
    10321 aggaatacaa aaacgactct tttatacaag tagctgatat ttttgataaa actgctatta
    10381 taattgagaa aatatccaat attataatag attatttaac tcaaacatgt gatgatacag
    10441 aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga tatttaatct
    10501 tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa tatgaaaata
    10561 tagcttgaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag gtgtggatat
    10621 gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat tacattatta
    10681 tgatgaaata ggtcttttaa aaccaaataa aattacagat gcaggatata gactttatga
    10741 tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg atattccttt
    10801 aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag ctctaaaaaa
    10861 tcaaaaaaaa ttacttattt taaaacgaaa gagattggat gaattgatag agcttataaa
    10921 tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga gcgagtattt
    10981 tgatgtttta gaagaattta aaagagagca taggaataag gtaattaaaa tttatggtag
    11041 tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga aaattgctaa
    11101 aatggctata aaacaatatg gaactattga gaagtttgct aaagctataa aaacaaattt
    11161 tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg attgtttaaa
    11221 agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt taaataggaa
    11281 tccttcttca acagaccttc aagaaatagc aaaagagata acagatatat ctaaaaaaga
    11341 ttatgaaatt ttctctatgg atactggtga tgacaactgg tattatatgg ttcaaaatta
    11401 tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg gagcaggaaa
    11461 gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga taaatacatt
    11521 gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag ttcaaagcgt
    11581 tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa tagacaatgg
    11641 tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact atataaaagt
    11701 aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt ttaagattta
    11761 ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt ttataaaagc
    11821 acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta ttaaaatata
    11881 tagtttacat caaacttaac actttcaata atatttccca aatatgtttt tatactttca
    11941 acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag aatattaaac
    12001 cttaatccta tttcatcaaa gtactcatga tttaaaactt tgtaccaatg gtttttttct
    12061 ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca atcttcattt
    12121 tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa atcatatagt
    12181 ttattgttaa cataaaatcc aaataataca aataagatag tccatagtat aacaaatgtt
    12241 gtagatttca atattaatca ccctttttat tttgaataaa tattttatca ttttcatcta
    12301 aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa atacccatta
    12361 accagttagt atctttttga gctatgtcta gatttttatt tattattcta ccatccagta
    12421 taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag cttgatgctg
    12481 gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct tttaggttaa
    12541 agtatccttg aatcctaagt tgctctaata attcatctat actaactctt tcttttttaa
    12601 gctctttata tacaattttt cccttatcta ttaatacaga aggcttacct gataaaaatg
    12661 aacttaattt tctgcttttt aatgataaga atgaaataag agtctgcata attacaagtc
    12721 cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt gggacagatg
    12781 caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca gctatttcac
    12841 cttttcccat gactctaagt gcaattagca ctgttatgta taatataata cttcttatta
    12901 atactactgt catatctatc aattactcct ttaaaaaata tttacactta gttagtatat
    12961 cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat tcaactattt
    13021 aaggtttttt tgataatttt aatattaaat tttttatgga ttttctgtta cttttattta
    13081 ttttaataac agattcaaca aaactctttt tatctcctaa ttttaagata aaatcaggat
    13141 attcttcaaa ttcattgagt tttattaatt tgaaagaaga agatagattt ttaaagttaa
    13201 taattttaga gttattcact aatacacttc ttttt
//
LOCUS       DAFKMX010000113        13231 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFKMX010000113 DAFKMX010000000
VERSION     DAFKMX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.113"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(58..201)
                     /gene="scfA"
                     /locus_tag="LZB08_002517"
     CDS             complement(58..201)
                     /gene="scfA"
                     /locus_tag="LZB08_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBM7316011.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(267..629)
                     /locus_tag="LZB08_002518"
     CDS             complement(267..629)
                     /locus_tag="LZB08_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBM7316012.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(687..980)
                     /gene="yajC"
                     /locus_tag="LZB08_002519"
     CDS             complement(687..980)
                     /gene="yajC"
                     /locus_tag="LZB08_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBM7316013.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1114..2235)
                     /gene="tgt"
                     /locus_tag="LZB08_002520"
     CDS             complement(1114..2235)
                     /gene="tgt"
                     /locus_tag="LZB08_002520"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBM7316014.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2314..2799)
                     /locus_tag="LZB08_002521"
     CDS             complement(2314..2799)
                     /locus_tag="LZB08_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316015.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2811..3836)
                     /gene="queA"
                     /locus_tag="LZB08_002522"
     CDS             complement(2811..3836)
                     /gene="queA"
                     /locus_tag="LZB08_002522"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBM7316016.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4154..5173)
                     /gene="ruvB"
                     /locus_tag="LZB08_002523"
     CDS             complement(4154..5173)
                     /gene="ruvB"
                     /locus_tag="LZB08_002523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBM7316017.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5280..5882)
                     /gene="ruvA"
                     /locus_tag="LZB08_002524"
     CDS             complement(5280..5882)
                     /gene="ruvA"
                     /locus_tag="LZB08_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBM7316018.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5875..6384)
                     /gene="ruvC"
                     /locus_tag="LZB08_002525"
     CDS             complement(5875..6384)
                     /gene="ruvC"
                     /locus_tag="LZB08_002525"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBM7316019.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6696..7325)
                     /locus_tag="LZB08_002526"
     CDS             complement(6696..7325)
                     /locus_tag="LZB08_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316020.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7528..7851
                     /locus_tag="LZB08_002527"
     CDS             7528..7851
                     /locus_tag="LZB08_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBM7316021.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8119..8973
                     /locus_tag="LZB08_002528"
     CDS             8119..8973
                     /locus_tag="LZB08_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316022.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9159..10595)
                     /locus_tag="LZB08_002529"
     CDS             complement(9159..10595)
                     /locus_tag="LZB08_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBM7316023.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10666..12048)
                     /locus_tag="LZB08_002530"
     CDS             complement(10666..12048)
                     /locus_tag="LZB08_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBM7316024.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12184..13089)
                     /gene="garR"
                     /locus_tag="LZB08_002531"
     CDS             complement(12184..13089)
                     /gene="garR"
                     /locus_tag="LZB08_002531"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBM7316025.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLVAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
BASE COUNT         4238 a         2425 c         1494 g         5074 t
ORIGIN      
        1 aatgataaaa gaatcacttt gagataccct cttacaaaca actaaatcaa taattttcta
       61 tctttcacac tcttgatttg caactgtaca agaagtttta caagctgatt gacaagaagt
      121 ttgacactct ccacatccac cttttgcagc actttgcttt aaagtagctt gagataaagt
      181 ttttacatgt ttattttcca ttttcaacgc ctccatttct aattgtttcc tattgattat
      241 atcataaaac tactttaata taaactctat ttaaagttga cacccaatac gcctccaatg
      301 cctcctgctg ctgatattaa caataactta taaatcatcc ctacttcaaa tacaaacttt
      361 tcttgtgcta aaaacacagt caaaaaaata catactatgt acaaaagtcc tactaaaagc
      421 ccatacataa gtcctttttc tttcatgtgt tttgaagtgt aaaaccctcc tatagctgca
      481 gaaatagtag ttataaatga tgagaccata gttatattat ctccaccaat atctgtaaat
      541 gtcaaaaata agttatacac taataaaaca gccaaagtta ttatatatgc atatcctaga
      601 cctttaaaaa tataattaga ttttcccata aaaaaacctc ctctcaaatt aatatatatt
      661 cgagaaaagg cttttaaatt actaatttag tcttcttcta tttcttcttt tttagattta
      721 acagttccta tagcccattt ttcaaatgga actttagttc tatttggtcc taattctaat
      781 attacaacat catcttcaac tttagctaca ttagctacta ttccacctat tgtaatgacc
      841 ttgtctccta cacttaggct acttctcatt tcttttaatt gtttgtcttt tttcttttga
      901 ggtctaatca ttaggaaata aaagattgcg attactacta cccatagacc tatactcata
      961 attatttgtt gctgtggcat taatttttca ctcctttgta aattatactc ttttgtaaat
     1021 cataataaat gtttatataa caatttactt tctctatctt tgagtactat tgaatattat
     1081 atattaaaat gattataatt aatagtgcaa aacctatatt tcatatccat attttgcaaa
     1141 aaattcattt ctaaaatcaa gtaatctatc ttccattatc gcttgtctta tttgtttcat
     1201 taaatttaat aaaaagtgaa gattatgagt agttattaat cttgcaccca gtatttcatt
     1261 tgcttttata aggtgtctta tataagctct tgaatagttt ttacaagcat aacaatcaca
     1321 ttcagggtca agtggagtaa agtcttcagc ataagttgca tttctaacta ctactttacc
     1381 ttggctagtc attgcagttc catttctagc gatacgagta ggtaggacac aatcaaacat
     1441 gtctactcct cttataacac cttctactaa gtcatctgga ctaccaactc ccattaggta
     1501 tcttggttta tcctttggca taagtggagt tgtatgctct aacacgtcat acataagtgg
     1561 ttttggttct ccaacactaa gtccaccaat agcatatcct ggtaaatcaa tattagttat
     1621 ttctttcgct gattgttccc ttaagtcttt atacatccca ccttgtatta taccaaataa
     1681 agcttgcttg tcggtattat tatgggcatc tttacatctt tttaaccatc ttgttgttct
     1741 ttctaaagaa ttttttacat attctctatc tgatgggtat ggtgcacact catcaaatgc
     1801 catcattatg tctgaaccca atgcattttg tatttccata gctttttctg gagttagaaa
     1861 atgttttgaa ccatctaagt gtgacctaaa ttctacacct tcttctttta tctttcttaa
     1921 aggccctaga ctaaatacct ggaaccctcc actatctgta agtattggtt tatcccaatt
     1981 cataaactta tgaagaccac cagctctttt tataagttca tgacctggtc tcatatataa
     2041 atggtatgtg ttactaagta ttatttgtga accaatctct tttaattctt ctggtgtcat
     2101 agattttaca gttgcttgag ttccaactgg catgaatatt ggtgtttcaa taatcccatg
     2161 tggagtgtgt aatcttccta accttgcacc actctgctta caagtcttta tcaattcgta
     2221 tcttactgcg tacataaagt tctccttaat catatttatt tctcattatt tcttaagata
     2281 tatttgtaat aagctattca tataatttat tgtctaaata gatttatctt cgtcttcttt
     2341 gttctgttgt tccttaattg cttcttcatc atgaatactc attatattaa gagcactctt
     2401 gtcttttgtt actaaaaact tttttatagc aaatagtcct gctatagcta taactcctat
     2461 taaaacatct atcatacctt catttttagg cagtagcaac atttttctag caattgcgta
     2521 aagtaatgcc tcaataaatg ctcctggtat atgaagagat agcattacta caagttcaac
     2581 acctattact aaaagtaata tttgtgctaa aaaatcatta agctgactgt attgtactgg
     2641 attttgaaaa tcaactatat atgctcccca cataagtctt agaacatcta tagttcctaa
     2701 aaatactgct attagaacta ctactgctaa aacacgttca aatataaaag caatctttaa
     2761 tatagggtta ttcttttttt gtttttgtaa actatgcatc cattacctcc ctactttata
     2821 atcatagcat ctccaaaact gaagaatcta tatctttctt ttactgcctc attatatgca
     2881 tttaaaacat tatcctttcc acaaatagca cttactaaca ttattagtgt agactctggt
     2941 aagtgaaaat tagtaataag tttatctaaa actttaaatt tataacctgg atatataaat
     3001 atttctgtcc atccactagt ttctttcatc tttccatcct cattacaagc tgattctata
     3061 gttctacaac tggtagtacc aacacatatt acattttttc cattttcctt tgctctattt
     3121 attttgtcag ctgcttcttg gctaaccata taatattctg aatgcatctt gtgatttaaa
     3181 acatcctcta cttttactgg tctaaaagtt cctaagccaa catgtagagt tacaaaggct
     3241 atatctacac ctttttcttt tattttattt aacaattctt ctgtaaaatg taatcctgct
     3301 gtaggtgctg ctgctgaacc attatgcttt gaataaacag tctggtatct ttctttttca
     3361 tctagtctag cagtgatata tggaggtagt ggcatatttc caagttcatc caatatttct
     3421 tcaaaaattc catcataatg gaattttata attcttgaac cttcatctga taaatctaca
     3481 acttctccaa ttagctttcc atttccaaaa gaaaatttag tccctacttt tgctcttttt
     3541 cctggtttta ctaaagcctg ccaagtatct tcctctgttc tttttaataa taaaaactct
     3601 attttacctc cagtttcaag cttctctcct attaatctag ctggtataac tctagtatta
     3661 tttaatacta agcaatctcc tggatttaaa tattctatta tatctctaaa tactttatgt
     3721 tcaatattac ctgtctcttt atctaatacc atcaatcttg agctagctct atcttctata
     3781 ggaacttgag ctataagttc ctgtggtaag tcaaatttaa aatcacttgt tttcaatgtt
     3841 aaacttcctc tcttaaaatt tctccagcta caataatatc aaaaaaacaa cttattttcc
     3901 acaatattac cataatctat caagaaattg ctatttttaa cagacaccat atccacaata
     3961 tatagatatt tttccacgat atacatgtct tatccacaat atatagatac aatacacaca
     4021 atttttatcg tgtttatcca cagtatctac agatataatt accattttgt ggatatgttt
     4081 gaatttttag ccattttaga aatattttaa tttaatgact cttttatttt ataaatactc
     4141 gtataatcta tttttatttt tcattaggat atggaatttt taagtgttca tatgctaatg
     4201 gcatggctac tcttcctcta ggacctctat ttataaatcc taattgaagc aaatatggct
     4261 catatacatc ttctattgta tttctatctt ctccgataga agctgccaat gtatctaaac
     4321 ccactggtcc tcctctaaat ttttctatta tagtcataag aagcttctca tctacaaaat
     4381 cgagacccaa actatcgact cctaaaagtt ccagagcatc ttttgccacc ttatcagtta
     4441 tttttccatc agctctaact tgtgcaaaat ctctaactct ttttagtaat ctatttgcta
     4501 tccttggagt ccctcttgaa cgttttgcaa gttctaatgc tccattagac tgtatctctg
     4561 catctaagat gcttgaagac ctaagtacta tttttgaaag ttcatcaact gtataatagt
     4621 ccaatttaca aataacacca aatctatctc taagtggatt tgtaagcata ccagctcttg
     4681 tagtagctcc aataagtgta aatttaggaa ggtcaagtct tatgcttcta gcagatggcc
     4741 ctttccctat tattatatct aggcaaaaat cctccattgc tggatataaa acttcttcaa
     4801 cacttctatt gattctatga atctcatcta taaataaaac atcattttca tttaaattcg
     4861 taagtatggc tgccaaatct ccagctcttt ctattgcagg tcctgacgta attcttaaat
     4921 ttacccccat ctcattggct attatacttg ctagtgtagt ttttccaaga cctggtggtc
     4981 catataaaag cacatggtct aactgctcat ttctcgattt agctgcttct ataaatatac
     5041 taagttgttc tttggatttt tcttgaccca aataatcttc taaagttttt ggtcttaaac
     5101 tgttttctat atctatatct tccatcttca ttgtagaagt tattatccta ttttcatctt
     5161 caaaaccttg catttatacc cctcctctca tacttttgaa aatctacttt atatattttc
     5221 aaatttacat aaactcctta aatattgttt taaaatccaa atattataaa atccaaatac
     5281 tatttcaagg atttactcat tatatatgta agtgctttct ttataatagc ttctgtattc
     5341 ataccatctt ttttacattt ttgaactgcc tcttttgatt ccgacaaagt atagcctaaa
     5401 gctacaagtg catctacaga ctcatcttgt gatatcaagg taggtttttg tgataataac
     5461 gttggttcaa actctatgtt atttttgtct accttatcct taagctctaa gacgattctt
     5521 tctgctgtct ttttaccaac accaggagct ttggataatt ttccaatgtc ttcacttaaa
     5581 atatatgctc ctaactgtgc tggtgatgca aatgatagta ttgaaagcgc cactttaggt
     5641 cctattttag atacagatgt tagtaattca aacatcttaa gttcttccct acttacaaaa
     5701 ccacaaagac tcatatcatc ttctcttaca attaatttag tatatatttt tgtggcttct
     5761 cccacttgta aattcattat tgtattggat gatacattta ttttatatcc tatcccatta
     5821 ttttcaacaa ctatactgtc tatataaatt tcttctactg taccttttat ataactatac
     5881 atacttacca cctatattct ttagagtttt ttcaagtttg tttgaatgag catggcaaat
     5941 agccacagct aaagcatctg caacatcatc tggttttgga acttttttta gacttaaaaa
     6001 tgaagttacc atctgttgaa cttgtgcctt atctgctctc ccatatccaa ctacaccttg
     6061 ttttacttga agtggagtgt attcatatac aggcttccca ttatgagcac atgcaagcat
     6121 agtaacacct ctagcttgtg caactgttat agctgttttt acattcttgt taaagaataa
     6181 ttcttccatt ccaacttcat ctatattgta attctttact attgtatcaa ttcctttata
     6241 cacaagttcc aatcttctcg atatattcat atgggcaggt gttgtaactg ctccataatc
     6301 gattgcttta aacttgctat ttttgtattc aattatacca tatccaacta tggctatacc
     6361 tgggtcaatc cccaatatta tcatttttta aaattccccc taaaaataaa agaaatacca
     6421 aaatgtattt ctcataaaaa cttatgttct aaatcattat atcataaata atattaacaa
     6481 atcataaaat gctctatgta aaatataaaa tcaaataaaa aagttatatc aaagcaagat
     6541 tttacctcaa tataactttt atatttatta caaataaatt attgtatttt tataggaatt
     6601 aaatgtgact acaattttaa tactaatcta tttaatatta aattatttat tattaattta
     6661 cttagtattt aacttaaaat aatattaatt tactattaac ttgcaaagtc ttctaatctt
     6721 gaataggcct cttcttcagt atccataact acaccttttt tgatttcatc ttgaactgtt
     6781 ttaggaagtg aatctactct tatttttgtt ttttctacta atgttttact tccgctatca
     6841 tcatatttat atagtgctat atttccatta tcattttcaa tagtatattt accttttata
     6901 gtaacatcca ttgcttgtac atctttatta tcactatatt tagattctac caatatgatt
     6961 tcatttttat ccatactctt tattttttta gttggatatt cttttttaaa atatgatact
     7021 atttctgact tcgtcttatc taataatgac tctggaacta ttcctgccat tgtaagtgtt
     7081 ctcttatagt tagtttcatt ttcgccttca ctttgaagcc aaacttcaca atttttattt
     7141 aatccaaaag cttcttttga cttttcattc atattcgcac tagtacttac aacttcacca
     7201 gtttgaactc cattgtgttt taatcttgtc cctatataaa ctccgctact taatacaaat
     7261 actccaacca gtattaaggc agatattttc caaggaaata attctttttt cttcttttca
     7321 aacattgatt acgcctcctt tttctattat tttctccaag ctgtaagttt ttatacaatt
     7381 taggtaaatt ttagaagaaa atatatttat atttcaaatt actctaagaa tagatatttt
     7441 ctaaaaggta gttttctctt aatggttata ttcttgtaat tatatggaca taatttataa
     7501 taaataatcg tttaggaggt aataactttg gaaagaataa actgtaatgt aattaactgt
     7561 tcacataaca acagccatat atgttatgca gataaaattt ttataaatgg tgaaaaagct
     7621 cacaatgaca gacatacttg ttgtagttca tttttagatg aatctattta tagtaattta
     7681 tctagcaacg ttaataatag aaattgtgag gaattagttt gtactgtaaa aaattgtaaa
     7741 tacaacaaag atggtagctt atgtacactt gataatatat tggttgctcc tgaaactgat
     7801 agagtaaatc tttatattga aacatgttgc tctagtttcg aatgtaaata gtcatagtat
     7861 aaatataagg gtgtcaaatg taattaaact aacatagtgt agagtatcta caactattag
     7921 tttgattata cttgacaccc ttatttattt taaataaaat ttcattatta taaccatgtt
     7981 tataatgttc taactgtaat taaatttgat aaatttctat aaattggtat aatcaatatg
     8041 taaattgaat ttagtaaatt aatcataaaa ataaaatatc gagtatatag tgttatacta
     8101 actagaaatg aggtgcttat gagtacattt tatattcaat ttctttattt tgtaatttat
     8161 agctttatag gatggtgttg tgaaactaca tattgttcag ttttacaaaa acaatttgta
     8221 aatagaggtt ttttaaatgg tccattttgc cctatatatg gatttggagc attagcaata
     8281 gttgctacct tgactccttt tgtacacaat attcctttgt tatttttgtt tagtataatt
     8341 ataactagca taatggagta ctgtacgagc tttattttag aaaaaatatt taatatgact
     8401 tggtgggatt attctaaaca taaatttaat attcatggaa gagtttgtct ggaaaactca
     8461 cttatgtttg gtgtattatc attgattgta atgcttatag tacatcctat tgtggttgac
     8521 tttattaact ctatctctaa aaatgtgcta tttatatttg ctatatctat agaaatttat
     8581 tttgttttag atttagttat cacagtccat acaattttac aattaaatgg taagttaaaa
     8641 caaatcaatc ttataattaa ggaattaaaa gataagaaag aatactacaa acttattaca
     8701 caagaaacta tagaaaacaa acttgaatct ttagttgaaa atagactgga tactttggaa
     8761 gataggtttg aaatacttaa agaaaaagaa aactatcgct atgctaaaaa tcgtattgat
     8821 gagctaaaaa ataaacttaa tactttgctg tctaaccaca aattacttca tagacgtata
     8881 atcaaagcat tccccaacat aagctctaat aagcatacag atattttaaa taaaatcaaa
     8941 gaaaatatta aatcaattaa aaaagataaa taataaaata aacacataag ttaaataata
     9001 aaaatactaa tacataagat taaggtttag aatatttttt gtaaaatact ctaaacccta
     9061 attaatatac tattattttt aatcttaatt tataatttta gtttatttta attttaactc
     9121 agtaaattta agcgtttaaa cttcaataca ctaatatact accccatcaa tatctcaact
     9181 gattctctaa gtgtgctaat ctctcccaca tttccaggga aaattatata tggcatatta
     9241 ggaaatttgc tttctttgcc tgtcatccat actggtatac caggttttat ctgtcccatt
     9301 actgtagctt tttttactct tagtgcttta gtaccaacat cactagaagt aattccaccc
     9361 ttagctataa taaaatttgg tcttacatta agcataccaa ttatactggt aactgcatct
     9421 gatatttcta cagaaatcat aagctgttta tctttatcat tcgtatccaa gtcaaaacgc
     9481 tctcgtctag tataaacagc aacagtttta cccctgctta ttttttcctc tacaactttt
     9541 ataactcttt ttacctcatt ttttagacca ttttcttgaa gaactaaatg ttgattaaat
     9601 tctataaatt ctatcggata tttacaattt ttaagttctt ctaactgttg ggtagtctta
     9661 ttcacatgtg aaccaacaag tataattcct ccattggtat taccttttga tactaattca
     9721 tccttagtaa gtagttcctt gtcactcact ccgccaagta cttttgttat ggcagcagcg
     9781 cttctaaaca taaattcctt accactattt actgctcgta taaacgcaat tgtaaatacc
     9841 tttacatcta cataatcggt tgcatttaca ataattttat tgaatccttc agctttttta
     9901 agtttttctg ttatagcttc aatatttagc tctctaagtt cttttgacga aatatatatc
     9961 atatcactag atttgtactt tccatttgtc ttttcttcac accactcacc tatatcagat
    10021 gatttatatc caaaagattt atcttttgca aattcagtca ttcctgctgg tacaagtatt
    10081 tctccttctt taacataatg tacattatct atagtaaacc taccaccttc tttaaaaaat
    10141 ggcattatta tttcaccctg aaataacttc cccgataatc tctctacttc gctttttaat
    10201 aaattagttt caagaggata atgtcctctc atagtagaat cactacgact tatgattata
    10261 aaatccttat taactttctt tgatacatcc acaatattct ttgaaatttc tttatgtgct
    10321 tttgtagttt gagcaacagt aaatcccctt gaattggtaa gtataaaaaa catactattt
    10381 ttctcattaa aaccttgttc catactatcc ttatcccaat cagtataaac agaaatatca
    10441 tgaactgttt gtacacctgt tgggtcatca tctaatacaa caatttttct atctaaatgc
    10501 ttaagctcat ctgcaagaag tttatttaat ctgtcctcat ctacttgttt aaaagtatta
    10561 agtatatcta tgctaattgg ttttacttct ctcatttaaa acgccccttt atgtaatttg
    10621 tatagtctaa atgtaattta tgtgatataa acaatagttt gttatttata gacctggaag
    10681 tggtataatt ccatttataa cactaagcaa taaaaccatt ataaatgcta caactccagc
    10741 aatggttcct ccaattgtcc aagagtttaa agtatcttta actgataatc cactaaatct
    10801 tgaaagtacc cagaatccag aatcatttgg aagggagaaa ccaactcctc cagcacatat
    10861 agcaagccct actaaaactg gtgacattcc agttgccaaa attgtaggtc ctaaaattga
    10921 tgatgttgtt actaaggcta cagtagcaga accttgagaa attctaagca aggcacttaa
    10981 taagaatcca agcactacaa ctgatatact aagaccagac atagtatcaa ctaagaagtt
    11041 acctatacca cttgcattta taacactgcc aaacgcacca cctgaacctg ttatcaataa
    11101 tattagccct gctgaatcag cagcttcaat aactacatct cccataggtt tatttatgta
    11161 tttctttaaa aatcctattg ccaccaatat tccaagaagt attgctatgt ttttatctcc
    11221 tataaatgca agtactccac tcattgtaga acctttttct aagaacatac taaatacatt
    11281 tgatataagt ataagaagta ttgggaacaa taaagaaaac atagaaattc caaaacctgg
    11341 tactttagaa gaatcatcat cttcaatttt tatgctatct tttccctgct ctaaattttt
    11401 ttctctatca ttctcatcaa tagcataagc tggatatttt tcaaaacgtt ttccataaat
    11461 ccatccacct ataagagctg ctggaaaagc aacaattata gaataaagta taaaactacc
    11521 tacatttgca tttacatttg ctgcaactgc taaaggtcct ggtgtaggta ttacaagtgc
    11581 atgacctact atagtcccaa ctcctaaggc acatacaaaa accattagag atttcttagt
    11641 tactctcgaa acatacttaa ttataggcat cattataaca aaagctgcat ccataaaaac
    11701 tggaattgaa attaaaaaac ctgctgtagt tactgctaaa gcactgtttt tttctccagt
    11761 cttagctaac aacccctttg caatagattc tgttgctctt gattctgaaa gaatatttcc
    11821 aaatattatt ccaagtccaa ttacaatacc tatactacct aatgtatttc caaatccagt
    11881 cgatattgtg gttgatattt catttatagg cattctaacc ataaagcctg taattataga
    11941 tgttatcagt aatgctaaaa acggatttac tttaaattta ataatcgata caagtaaaat
    12001 tcctatagat ataacaaatg ctatcaaaag tccatatcct gtaatcataa tctttacccc
    12061 tttatcattc tactttatta tttatcaatt ttatttttct tttactatga taacttaacc
    12121 tcgctgtatt tatataatct aatcctaata atatgtattt aattatattg atatatttaa
    12181 atattaatta tcgtgttttt ttatttcaac acctgccaac ttttcaaaat actgtgctat
    12241 tccactatgg tcatcttcca aatgtccatg tacttttaat gtctgcataa tttctaataa
    12301 ttgacttgtc ataggtagcg gtacatctaa attgtgtgct gtttgcatta cattcttaat
    12361 atctttcaaa ttaatagata tttttccacc tggtttaaaa tcacgattca taatcattgg
    12421 tatcttcgca tccaatattg tactaccagc taggcctcct cttattgcct tatatacttt
    12481 ttctggatct gcacctgctt ttgctacaag tacaaatgcc tcagaaactg ctgctatcgt
    12541 caaattaact attacttggt ttgtaagttt tgtaacactt ccactaccat tatctccaat
    12601 taaaatagat gagcttccca ttatattaaa aaatggctct actttcttat aaatttcttc
    12661 cttaccacca gccataaaag caagtgtacc atcaatcgct tttggctccc ctccacttac
    12721 tggtgagtct ataaattcta agcccatatc ttttaattta tttgcacaca atatggattc
    12781 tgtaggagta actgaactca tatcgactac tatacttcct tcttttaaag tttttgccaa
    12841 tccatccatc ccaaacaata tatcctgcac aatagttcca tttggaagga ttgtaaatac
    12901 aatatcacat tcttgaccca tttctaaaac acttgcgtat tttgctccac atgatataag
    12961 ttcatccaca gcagatttat taatgtcata caccaaaaga ttacatccat cttttaataa
    13021 attttttgcc attggctttc ccattattcc taatccaata aatcccaatt tcattttata
    13081 accctccata tcttcacatt tttgaaaacg ttttttatgt tttagagtat actacatacc
    13141 agtataccgg tcaatataaa atataaaagt atttatttta acaataaata gtaattttta
    13201 aagtcattta ttatatttag tcatcctata t
//
LOCUS       DAFKMX010000114        13187 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFKMX010000114 DAFKMX010000000
VERSION     DAFKMX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.114"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            263..1144
                     /locus_tag="LZB08_002532"
     CDS             263..1144
                     /locus_tag="LZB08_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7316026.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLRERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1352..2215)
                     /locus_tag="LZB08_002533"
     CDS             complement(1352..2215)
                     /locus_tag="LZB08_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBM7316027.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2370..4304)
                     /locus_tag="LZB08_002534"
     CDS             complement(2370..4304)
                     /locus_tag="LZB08_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBM7316028.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEVDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYNST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4416..5429)
                     /gene="galE"
                     /locus_tag="LZB08_002535"
     CDS             complement(4416..5429)
                     /gene="galE"
                     /locus_tag="LZB08_002535"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBM7316029.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHIVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5453..6322)
                     /gene="galU"
                     /locus_tag="LZB08_002536"
     CDS             complement(5453..6322)
                     /gene="galU"
                     /locus_tag="LZB08_002536"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBM7316030.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6520..6840)
                     /locus_tag="LZB08_002537"
     CDS             complement(6520..6840)
                     /locus_tag="LZB08_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316031.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6830..8821)
                     /locus_tag="LZB08_002538"
     CDS             complement(6830..8821)
                     /locus_tag="LZB08_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316032.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8944..11487)
                     /locus_tag="LZB08_002539"
     CDS             complement(8944..11487)
                     /locus_tag="LZB08_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBM7316033.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEMEQNEL
                     KEIKQKEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11765..12099)
                     /gene="rnpB"
                     /locus_tag="LZB08_002540"
     ncRNA           complement(11765..12099)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LZB08_002540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12231..12908
                     /gene="srtB"
                     /locus_tag="LZB08_002541"
     CDS             12231..12908
                     /gene="srtB"
                     /locus_tag="LZB08_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBM7316034.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13068..13142)
                     /locus_tag="LZB08_002542"
     tRNA            complement(13068..13142)
                     /locus_tag="LZB08_002542"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13108..13110),aa:Thr,seq:tgt)
BASE COUNT         4064 a         2304 c         1347 g         5472 t
ORIGIN      
        1 cgagacttta ctaataacaa attgataatt tcctattaat tcatagattt tgtttataga
       61 ttttatttat aagataattc ttgtattttt ctattaggtc gtataaatat atttatctta
      121 taattcagac aaaaaacgat aaattttttt aaaagctttt attcttactc tataatcata
      181 tataataaaa atgtaaaaat atcttaaagg ttaacttcta caaatatata aataaaaata
      241 cctatgaaag gatgaacaaa aattgaattt atatcactta cgctattttg ttacattggc
      301 acatttagaa cactatacaa aagcggcaga aaatttatct ataacacaac caagcttaag
      361 tcatgctatt tctttactag aaaatgaact tggggtggct ctatttgaaa aagaaggtag
      421 aaatatagtc cttactaagt atggaaaaat atttttaaaa gatgtggaaa aatcattaga
      481 aattttagat tcaagtgtaa aatcattaaa aataactggt actggtgaag gacaaataga
      541 cttagctttt cttagaacac ttggtactga ttttatccca gacatagtgc ataaattttt
      601 aaaatctaat cctgctaaat caatagattt taaatttcat actggtgtaa ctactgacat
      661 tattcaaggt cttagagaaa gaaaatatga tattgcattt tgttcgaaac ttgaaaaaga
      721 aaagggaatt gaatttatac ctgttgccaa acaagattta gtactaattg taccatatag
      781 tcatccacta gctgcaaagg atactataga tttaaaagaa accatacctt atcctcaaat
      841 agtatttaat cagagaagcg gtcttagata tataattgat gatatgttta aaaaaataaa
      901 tcaacagcct aatatagtct atgaagtaga ggaagaccaa gtaattgctg gattagttgc
      961 aaaaaatttc ggaattgctg tagtcccaaa tatgaatatg cttagcttta caaaagtaaa
     1021 agtaattcag attattcacc caagttggga aagaaacttt tacttagcat ttataaaaga
     1081 tagatatttg cctcctgcaa ttaaaaactt taaaaacttt gtcattaaaa acgctcaatt
     1141 ataacttatt aataaagggg ttatttttaa atatcctctt ttattctttt tataaaataa
     1201 aaaaatctat acttttaaaa gtatagattc ctaactgaca gtcggcatcc gagtctcagt
     1261 aattatcgtt atacaacagc aactggattc agttcctgaa taactaggtt acttacatta
     1321 tatcagattt tttaaaattt gtcaattgta tctaacgcat tttttcaagc catggtccaa
     1381 caggctctac aataggaact tctttaaatc cccaatttct tatatcattc acactcgttg
     1441 gagtatatat tttgtttctg acagatttga atattttgtt tttttctcca tattgcatac
     1501 actttggatt agcttctatt aaccctcttc ttgctctgct aagaggatta cataaaatat
     1561 ctgctatttc taaaccttct ctatagtcag catcttcttt tttggcaatt ataaaaccct
     1621 taaccttatc tcttaattct tcatctatat ctaagtatat tgttccatta ttaaatacat
     1681 caaaaaacgc cttttgtact tttaagtttg agacatcatc ccttgactct ataactattc
     1741 gtgcagattc tacagtttca ttatttacaa atgaataaaa attctgaaga acatgtataa
     1801 aagccacaac atacggttcc tttgaaggtg cataatattt ctttaatcta gctttatcta
     1861 ctgttactga tattatagta cactctaatc tagatagaaa taaaggcaac tcatcataga
     1921 ataacttaat ctgtgaatca tcaaacttct caaatgctct tgattttctt gatatatctg
     1981 tcaaatgtat tcctatgtta ctggttccaa aacaatactc tttaaatgca tccatttctt
     2041 tcctcaaaga acatacataa tcgccttgac ttactgcata tttatattca aaaattactc
     2101 ctgtcaatgt aaatgggtct tcatgattta tatcagctct tccactttca tcaataaaca
     2161 ttatatattt tttctttata atcttactca ccctcttaac atatgatgat agcatctata
     2221 ttcattttat atgagtttta aaaatattcc aagattttta tataacttaa acttaatttt
     2281 atactaaaaa attgagatgt aaaacaatca agcttttatt gattgaattt cacatctcaa
     2341 ttataattat ttttcaatat atatttttac tattttgcaa gaatatccat taatttatcc
     2401 attactatat aagatacccc gcctcctatt tgataaggag tttcaacttt tttatttgca
     2461 aatactcttt gttgctctga tgataatcct gaattatcca ttaaaactat tggtgaatgt
     2521 aattttacag ctaatggtcc agctgataaa gcatctacta aagcatctga tttactaact
     2581 aatacagcct gtaaatcagt atctccatag aatttttcta ttactttggc attagtatct
     2641 actctactat ctccatatat tctatttcct gaaatatctt cagtagttat atcattcatt
     2701 tttgctatta ctgaatcgtt aattgcactt ggtccaccta taaagtatgc attttctaat
     2761 tttctttctg ttagccattt ataagattct tctgtaattc catctttatt gactaatatt
     2821 ataggttgtt tttcttcccc agctttagaa gctacagata aagcatctgc ttccccattt
     2881 ccagaaacca tatatgcagt ttttatttct tctcgatttc caagttcttc agctattctt
     2941 gtagcagttt gatatctatc ttgacctgct attctctttg tttctgtgtt gaaagtagtt
     3001 tttatttctt gctctaattt agaacttatt gcattttctc cacctatcaa gattactgtt
     3061 ttaggtgcta ataacttcat ttcttcaaca gtttcagttg gtaaattagc tgtattagta
     3121 agtagtattg ttgagttata gctacttgct aaaggagttg ctgttatacc atcagctatt
     3181 gcattaccat ttactaatat tacagtatct gagctatcag cattccagtt tccaactgaa
     3241 cttatctttt tagctgtatc aaatctattt gcacctataa ttgtatccca tgtaggattt
     3301 atagttccat cttcattaaa agtataatct ttcccatcta ttttttgaga accagttagc
     3361 atctttccat ctttgtctaa atagtaagac ttaccatcta tttgttgcca tcctgtctgc
     3421 attattcctg ttttgtcaaa gtaatactta ttaccatcta cttcttgcca tcctgttact
     3481 gctaaaccat cagatcctag atagtatttc tttccattct tttcttgcca tcctgtttgc
     3541 attactccat ctgagttaaa gtaataagtt tttccgtcta ttactttcca tccagatact
     3601 cttgctccat cagttcctaa ataatatttt ttgttattaa ttgttttcca tcctgtttgc
     3661 attactcctg aagcatcaaa gtaatacatt tttccattta cttttagcca actattcttt
     3721 tggtatgttc catcagattt tacataatac ttcttaccat tttctgtttt ccatcctgtt
     3781 tgatttagat ttatcttata ttttgcggct atctttacat agctgtcatt actataatct
     3841 ataattacat ctgaacctac aggaacttgg tcaaataacc atctaacatc tttattgtgc
     3901 attcttatac atccaccaga aatatgcttt cctatagaac tctcattatt atttccatgt
     3961 attccatatg tatctccata agttcctttt aatgctaatc ccatccatct atctcctaat
     4021 ggatttcttg gacttcctcc aggtatatta cctttataat atggtctatt ttttatcttg
     4081 ttaactactt ttgttttccc tgttggtgtt tctgacccct ttttacctgt tgcaactcta
     4141 aattctttta caagtttatt atttacaaaa tatcctagag tattttttct tgaatttact
     4201 ataatcacat gtttactggc tgcgcttgat tctggtacgc cacatgcaaa tactaaaact
     4261 aaagtcagtg ctagtactaa atactttttt agactttttt tcactataac ttccccctct
     4321 tttagaaaca ttactacatg tgtctaatta taacataaaa ttaccaatac cttatattta
     4381 caaatttgtt acatttatac atataagtat atattctatt cttcataccc attaggattc
     4441 atactttgcc atctccatga atcttcacac atctcttcaa gttcatactt agctttccat
     4501 ccaagctctt tttctgcctt tgaagaatct gcataacaca tggctacatc tccagccctt
     4561 cttcctacaa tcttatatgg tatttctttt ccacttgctt tagaaaatgc ttttactaaa
     4621 tcaagaacac tatacccctt tccagttcct aaattataaa caacaagtcc tggattttct
     4681 tctaattttt gtaatgcttt tacatgacca gcagctaaat ctaatacatg tatatagtct
     4741 ctaactccag ttccatcatg tgttggatag tcattaccat aaacactcaa ttccttcaat
     4801 ttacctactg ctatttttgt tatataaggc attagattgc tcggaactcc atttggttct
     4861 tcacctattc taccactttt atgagcaccg actggattaa agtatcttaa aagagctata
     4921 tccaatgact tatctgcttt tgatatatct actaacatct gctctatcat aagtttagtt
     4981 cttccatatg gattggttac agaaagtggg aaatcctcta atataggaca agtatgtggg
     5041 tctccatata cagtagctga agaactaaat acaaactttt taacaccata ttctctcatt
     5101 aattcaaata aatttaaggt acttattaga ttattgctgt aatattcaat tggtttttct
     5161 actgattcac caactgcttt taaagctgca aagtgtataa ttgattcaat attattttct
     5221 ttaaatacaa tatgcatttc atctttattt ctgatatcaa tattataaaa ttttactggt
     5281 tttttagata attccttgat tctatcaacc actattgagt tgctattact taagttatca
     5341 actataacaa cttcataacc tgattctaaa agttctattg ctgtatggct tcctatatat
     5401 cctgctcctc cagctactaa aactgccatt gtaattacct cttttctaat atttattttt
     5461 cacttacttt ttttctcata tagtccatta aatcatcttt taaattttct ctctttagag
     5521 caaagtctat tgttgcctct aaaaaaccaa tcttatctcc cacatcatat cttctacctt
     5581 caaagttata agcataaata gcctcttgtt ttccaagagt ttgaagtgca tctgtcagtt
     5641 gtatctctcc acctttacca ggtgtctgtt tctctaatat gctaaatatt gcaggagtta
     5701 ttatatatct tccaagtata gctatatcag atggagcttc ttcaattgca ggtttttcaa
     5761 ccatatcttt aactttatat actctatctt cgatatgttt tacatccaat ataccatatt
     5821 tattagtatc ttctttagct atcttttgaa cacctaatac agttgtttta tactcattat
     5881 aagtatctat taattgtttt aagcacggaa catctgaatc tactatatca tcacctaata
     5941 atacggcaaa aggttcatca cctataaaac tttttgcaca gtaaattgca tgtccaagac
     6001 cttttggctc tttttgtctt atataatgaa tatttaccat atttgatatg tctcttacca
     6061 tttctaacat ctctgtctta cctttttgct ctagttctaa ctctaattca actgatttat
     6121 caaaatgatc ttctatagat tttttatttc tacctgttat tataagaatt tcttcaattc
     6181 ccgattctat agcttcttct attatatact gtaaagttgg tttatctact ataggaagca
     6241 tttctttggg ctgtgatttt gttgctggta aaaatctagt tcctagccct gctgctggta
     6301 ttattgcttt tttaactgtc atctgcattt tttcaccctt cttcgtaaat tttctattgt
     6361 accaaattaa tataccatat ttatactttt tcttcaaact ctatattatc ccaatattta
     6421 atgctataag ataatataag gtagatgaaa tttcatctac ctttagtttt ataagttaat
     6481 attgtattgt ttatatttta tccaaaactt atacaggtac taaatttcat ctaagatgcc
     6541 ttttatatca ctatctatat cttcatctag ataagaatct aaatcttcta agtcaacttc
     6601 tagttcttga tatgtcatat caacactgac atcctcatct atatccattt ctgatattaa
     6661 ttctcctaaa atactatttc catcagaatc taaaccacta tctatatctt catctatata
     6721 gttttcattt aaaatttcat ctaaatcaat ctcgtaataa acttcattat ttttttcatc
     6781 ccctgagtcc aatcctaaga actcatagtt aaaagtccaa acattgtatt tacttgacat
     6841 actttattcc tcctgtgaaa aacatatttt catctagttt attggttcca taatcaacag
     6901 agttatatat tttttcaatt atgtcttttc ttctttcttt tgtactatta tttaatgtca
     6961 attgtttaaa tacatatgta tttgttattc tctcgaataa tatttctttt atactatcat
     7021 catcaatatt tctatagttt ttccacaata actcaaataa cttaaacatt tccctactag
     7081 tttcatctac ataataagga tacatatttt ctactaaatg gtttaatctt gatatggctt
     7141 gttttaaaaa tacatcaaga cttgcacttt cactttcttt taacattcta taattgtatt
     7201 tcttcttatc ttctcctgaa attacaaaga tgatattgtc taaacttttt tcaacttcat
     7261 ttaaataact gcttatatta tatgataagt cagacaatag atttttaatc tgcttaaaca
     7321 cttgtacatc aagtttagta ttatactttt caaacaaatc tatttcttga ttttctaata
     7381 ttcttggcat atatgctgac aataaatcta aattgtttct ttgttcttgt atttgcttat
     7441 aaagtgaatt tataacttct attcttattt catcatttaa ctctttttct aaaatcttat
     7501 caaattcaga tttcatatag taaatatttc caaagaaatt aaatattatc atatcattca
     7561 tagctttaga ccaattctta tatatcactt caatagaatt ttctttaagt agaggttgct
     7621 ttgcatatat aaatacatct tgcataagtt tttgaaccat atttttactt aagtttaaaa
     7681 gtactttttg attattaaat tttatttccg tttctacaaa attagaattt tcgttttcaa
     7741 gttctttatt aattacaatg tcttttatat cactattttt tatagatatg atttcttttt
     7801 gaaaagactt tagaatagtc aagctatcga aatcttcttg aataaaaaat ggagcatgtt
     7861 ttaatgtata tcccactatt tcattttcta tatcttttag gtcatctatt tgcataattt
     7921 ctctattttt atcatttata tgtaggttaa atacattttt attatcatat aatattatat
     7981 cttctcctaa atttaactta gaacaattat ctttcgctga ctttacaata gtcttcttag
     8041 atttcttatt tataaaaatt aattggtctt gatattttac taaaagcatg tttgtgtttt
     8101 tataatcaaa tccatctata tttcctgata tcttgcagaa tactatttca caatctttag
     8161 gaagatttcc tatcttttgg ttatctttca ctctctcatt taagtcagat gggactacac
     8221 tatctatatt gttattaaat atagatacag accttactat aatcccctct ctttccatat
     8281 gaaagtatcc cttcaatgac actcttttat cttctaaaat gtatctttct atatcatcaa
     8341 aatttatttc aaatctattt ttcccactga taaatataaa cttctcatta tctttattaa
     8401 ttccacccat ataaaactta ttgtcatcta aacttaatat agtcatatta tcctttagat
     8461 agaatataaa ttctctttga cttttgcaga ttaaatcata ttcatccaaa gttattttca
     8521 tagtcaaatt tgctgtaaca tttttacttg aattttcact ttttatgtaa aaaggcaagt
     8581 tatttccata gacaattatt tgagaatctt ctaaaactgc ctctaatttt tctttattgt
     8641 ctgaaaaact atatatatat tttacacctg tatcctcatt aacttcatct gatttgctga
     8701 ttattatatt ccctagattt gttatatgaa gctttttata ttcttttgag tatctactta
     8761 tgccactaag ctcaaaccca tctgaaagct catatatgat tttttctcct aatttcaaca
     8821 taccttcacc acctaaacag aattataagt attttataaa ggtgaataaa atgtctcaaa
     8881 acaaataata ttttttttaa tactttttat tctgagacat agtttctcct tttttataat
     8941 gctctaagca ccttcaacta tttcttcata aagtttatct aaatgtttaa catattcacg
     9001 atatccagat ttgttcttta actcacttct taaatcttca tatttttgtt taagttcttc
     9061 agcactcaaa gaaggagctg attcttttaa ttcttctaac ttataatcat agtacttatc
     9121 atacatagct atatatgtat caatagactt cttagctagt tcattacatt tttcttgtgc
     9181 ttctccatat tgagaataat cacctatttt tattttatag tatccatttt cttgaattat
     9241 tgttgaagaa atacctttag cctcatattc ttttgctgtt tcttttgctg ttttttcaac
     9301 tttaagtgaa cctgcataaa cagtgtaata tacaccttta ccatctttat atgcttccat
     9361 ttcttttgct ttcttttcag cttgtgcttt ttcttgagcc tctttttctg cttttgcctt
     9421 ttcttctgct tcttttttct ctttttcagc ttgtgctttt ttctcttctt cagttaattt
     9481 attgttagct tgttgttgag ttgatgactt tggaatattc tctttatcat ttttcttatt
     9541 gttgcctact gttataaaat atcctacacc aacaataacc actataacta ctgctataat
     9601 agtttctttt aatctactct tcttagaaac aacctcttca tcatctaaag aatcttcttt
     9661 atctgataca taatttttca tttctttctt gttttctcta tatttgtcct tagaagaatt
     9721 tttagatttt ttattactat tttctttctt tttaataggt attactactg atttttcatt
     9781 ttctaatttt tcttctttta tctcttttgc ttttttatct tctaagtttt tactttcttt
     9841 ttcaagcttc gattcattat ttgaagatac tttttcatct aagttattat ttattggttt
     9901 ttctattttt acattacgtt cttcttctat atgttgactt ggttcttttt gttttatttc
     9961 ttttaattca ttttgctcca tttcttctga ttttacttga tgattttcaa ctggtttatc
    10021 taaaattata tcaggagaaa tatcatttaa attctcttct tcttgttcaa cttcttcatt
    10081 gtctttttta ataaatccaa ataatttagc cttcttttta ctctttttat tttctggttc
    10141 aattatatct tctttattgc tgtcgtactc tttatctgta gtatctttgt cttctacaac
    10201 gtcacctata tcttgattat tactatctat ttcttcttta accccttctt cagcatcatc
    10261 ttctattgaa atactacttc taaagcaaat atctttatct gcatttttta tcatgtaaat
    10321 gtattcttca atatcgatat ctttatttct tctagttctt tttgatgcaa tttcatgatt
    10381 ttctaattca ctatagttat cttttattag attctgtgct ttattccatg tatcttcact
    10441 aatttctgtt ttatttttaa ttacatagtc cacaaattcc ttaaaatttt caggaccaaa
    10501 ttctccaatt atagtcagtc tcattgcacc ttctataagg tctctatttc ttctctttaa
    10561 aaattctaat acaaccgttg cacaagcaaa atctttaata atttctttgc caaaaccata
    10621 tccttcaaca gaatgaagtt cttctaatcc acataacttc ttatattcat tgtgtattgt
    10681 ttgagataca ctttcattat ttaagctatt taacttatct ataaacttcg attctatatc
    10741 atttgatttt gattcatgtt ttacttttga atttttaatt tcatttagta gcatgtcatt
    10801 ttctgaatta tctttaccta attgcaaatc ctcaatttca gttgctttta tttctacttt
    10861 gtcttgttcg ttattctctt ccacattatt aggtaccaca tctttattgc tagctaatat
    10921 tttgtttaca cagaatataa tttctctttt taattctctt ttcgcatctg cttgattttt
    10981 aagaaataaa tctccacatt ctttcttttc taaacttatt tttctaagag atatattaag
    11041 aaggtcttta tataggttta aaaccttata atccttttct aaacgtcttc ttctctctga
    11101 tagttccatt ttatcataag ctaaaaaact atttaaatca ttggctttta gagagtctaa
    11161 tccctctttc atagccttat ttacatactt tctacatttt gctgtagtta attctaaatc
    11221 tttttctaat tcatctattt caggtatgta cttatctact atccatttgc caataagtgg
    11281 ttctgtattt cttgctaatg tcttataaaa caatatatta aattttaaaa gctctgcata
    11341 ttttgcttca ttaaattcta ctttttcaaa tttcttttcc aaaacgccat ctagcttttc
    11401 atttatttta gactcttgtt ttaaaaaact tgctgtgaca tttaaaacag tgtttcttat
    11461 tatattattt tcactcaaaa ccttcactcc ttaaatcaat tattttattg taatatttac
    11521 attgtgcaaa gttgctaatt tgattatgat atctaaataa ttatttacta aatttgctac
    11581 atcttatatc atatcatatt atttacatca ttttactttt tctttttata tctatcttat
    11641 attataccat ttttaatatc tgtactaact tatattacaa tattcctcaa aattttaaaa
    11701 gtagattaga caaatttaca aataaaaaaa catcagttta aactgatgtt tttgactatc
    11761 gaaataatta agcaagtcta taagcggagt tctgtattag ataatcatct ttctaggcct
    11821 attgtcacca ataggctcaa gcgacctacc taccggagag tacgagcagc actctttatt
    11881 cctatcttgg tcttgctcca ggtggggttt acatagccaa ctagtcgcct agctgctggt
    11941 gagctcttac ctcgcctttc catccttacc acaatagtgg cggtttattt ctgttgcact
    12001 atccttagga tcgctcccac taggcgttac ctagcaccct gctctatgga gctccgactt
    12061 tcctcatgta agattacatg cgattacctg gcttacttaa tatttatttt acttagttat
    12121 gttaacacaa attcttttac atgtcaaagt aatattatac taaaaattta aatttattca
    12181 aaaatagtgt aatatagata tataaactac atttaactcg aggtgatata ttgaaaaaat
    12241 tatatagaat agtaattaac attatactag tattagtaat actttacagt ggatttaata
    12301 tatactcaaa acttactaaa tacaatcatg atactaaaat atcctcagaa cttcaaaaaa
    12361 aagaatataa aaaagaggat ttatcaaaaa ttaacagtga ttttaaattt tggctttctg
    12421 ttgagaatac aaatataaat tatccagttg tacaatctaa agataattct tactatcttg
    12481 acaaagattt ttataaaaaa gattctattt ctggtacact gtttatggat tatagaaata
    12541 aatctattga tgacaaaaat atcataatat atggacataa tatgaaaaat aaaactatgt
    12601 tcaacaacct aaataagttt aaagatgctg acttctttaa aaaaaataat aaaataaaaa
    12661 taaccttgaa tggaaaagaa tttttatatg atgttttttc tgcttatatt gtagaatctg
    12721 attatgatta tctaaaaaca aattttaaca atgaatctga ttatcaaaat tacataaatg
    12781 atattacttc taaatcattg tataaatctc ctattaaggt aaatagcaat gataaaatag
    12841 ttacactttc tacatgtacc tatgaatttg atgatgccag aatggtgatt catggtagat
    12901 tgatttaaaa ctcacagaca cagtttaaaa caataatttt aaactgtgtc atcttttata
    12961 cctataattt gtaaattaga ccactaaaag tatttaatac aaaataacaa atacatcaaa
    13021 caataaaaaa caataaaaaa agactatgct aaaaacatag tctttactgg agctggtgat
    13081 aggaatcgaa cctacaacct gctgattaca agtcagctgc tctaccgttg agccacacca
    13141 gcacacccat tttattgtaa attagtaaaa ctaattttta aaagtta
//
LOCUS       DAFKMX010000115        13076 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFKMX010000115 DAFKMX010000000
VERSION     DAFKMX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.115"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(150..1364)
                     /locus_tag="LZB08_002543"
     CDS             complement(150..1364)
                     /locus_tag="LZB08_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBM7316035.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1608..2990
                     /locus_tag="LZB08_002544"
     CDS             1608..2990
                     /locus_tag="LZB08_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBM7316036.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            3002..4351
                     /locus_tag="LZB08_002545"
     CDS             3002..4351
                     /locus_tag="LZB08_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBM7316037.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTHFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            4501..5034
                     /locus_tag="LZB08_002546"
     CDS             4501..5034
                     /locus_tag="LZB08_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBM7316038.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            5118..5927
                     /locus_tag="LZB08_002547"
     CDS             5118..5927
                     /locus_tag="LZB08_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7316039.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            6338..8383
                     /locus_tag="LZB08_002548"
     CDS             6338..8383
                     /locus_tag="LZB08_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316040.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            8644..9843
                     /locus_tag="LZB08_002549"
     CDS             8644..9843
                     /locus_tag="LZB08_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBM7316041.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKYGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            9895..10590
                     /locus_tag="LZB08_002550"
     CDS             9895..10590
                     /locus_tag="LZB08_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBM7316042.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            10603..11280
                     /locus_tag="LZB08_002551"
     CDS             10603..11280
                     /locus_tag="LZB08_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316043.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            11391..12992
                     /locus_tag="LZB08_002552"
     CDS             11391..12992
                     /locus_tag="LZB08_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBM7316044.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKFWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         5038 a         1391 c         2371 g         4276 t
ORIGIN      
        1 ctaaatttta tcagttatat tttaacattt tactttatat ttaattataa ataaagtatt
       61 tttaaaagga acattataaa agactgtatc aaagttaact tttagaatta taaagtttca
      121 tctttttaca gtcttttaat atataatatc tatttatcaa atctatctac tttaaataat
      181 ttcatatttt tattttgttt gccaagatat aattcactta acattatacc tcctaatatt
      241 gcaaagagta taccatttgc tccataacct aagcaatacc atagattgtt atttttatta
      301 tctggaccaa taaaacctaa attatctaaa gttgaagcaa aacaaccaca atatttatat
      361 tcaacttcaa tgtcttttat atccttaaac atacttttca atctttgttc taaaatatca
      421 tacttttcat tggctaaagt ttcattttct atatcatcaa aacttacatc ttcaccacct
      481 ataattaatc gattgtcttt agtagttctt agataattat atgggtcaca gttatctcta
      541 attaaaatgt tgtttttcca agaagctatg tttttaagtg gtttagttgc tatattaaag
      601 gtatttgact tagttgcaaa atttcttttt gtaaataaac ttgtattgta ccctgtagct
      661 acaattaatt ttttaccatg aactttgtat ccatacgata cttcagcagt aaccttatca
      721 tttgataaat ctactttttt tacttctgta ttttcatata cttgaagacc attttttaaa
      781 cttacatcaa tcaaatggtg actatattta tacgggtcta attctcgtcc tccattcttt
      841 gctattaatc ctgattttaa atcaaagcta aaagggtttg tggattcatc aatatattca
      901 acatcaaatc cattttcttt tcttaagtta tattcttctt tgatagcctt aacttcaagt
      961 ttgttggcag tataaagtag agtatctctt ttggcataat cacacttatt tccatatagc
     1021 tctataaatg tatctaattc ttcaagtgct gaagtacaaa ggttatatgc ttttagagca
     1081 tcttttagag tcataacctc tgttaaatct atcaaattgt catctaattc atactgtaat
     1141 agagaagttg tcacgctagt gcttccatgt gcaatccttc ctttttcaag aatgacagac
     1201 tttatattgt ttttagctag atagtaggca caaatacatc cagttacgcc accaccaaca
     1261 attataacat cagtatcaat atcatcagta aggtatggaa attgtcttgg aactttatta
     1321 atattggtaa aaataggatt tccttttaca tattcttgat tcatatactt cactcctaaa
     1381 atttattatg tatgagtgta ttattttcta tagcaaataa tattatacat tttaattatc
     1441 tagttatatg ggtatgtata gataaaatgt gataacattt tagtataaaa catatgtata
     1501 atatattcat aattacctta ttttaagtaa tatagtatat aatgtttgtt aacaagtaaa
     1561 aagattgaat aaaagttaat taaaaattta aagtaggagg taaatttatg ctttcaaaaa
     1621 atatagcttc taaggtatta gaaaaatgtc taattactgg aggagatttt gctgagattt
     1681 ttgaagaaga cactataaat aattctatta gtttaataga taataaagta gaaaatgcaa
     1741 taggtggaag aaattatggt ataggaataa gaatatttaa aggattgaaa agtatatatg
     1801 cttatacaag tgacaattca ttaaatagtc tgttggatgt agcatacaaa gctgctgttg
     1861 ctttaggtaa attggaagat ggaaaaagtg taatattaaa tgacagtatt aaaataaata
     1921 acatacataa tataaaaatt tatccaaatt caataggaaa tagagataaa gtaagtgtta
     1981 tgaaggttgc atataagagt gcaaaagaat ttagtaatga tatatctcaa gtaagtgtta
     2041 gctatttgga taaagaccaa aaaatattaa ttgcaaacac tgaaggtata tatgttgagg
     2101 atagaagaat aagaaccaga ctggggataa gttcaattgc atctaagggt aatgaaaatc
     2161 aaactggatt tgaaggtcct gggggttgta aagggtttga aatatttgaa gaaatagacc
     2221 cagaatatta tgcaaaagaa tcagctagag tagcgcatac tatgttgcat gctaaaaatt
     2281 gtcctgcagg taatatgatg gtagcaatag ataatggatt tggtggagta atatttcatg
     2341 aagcttgtgg acattctttg gaagctacat cagttgcaaa ggggaactct gtatttgcag
     2401 ataaattagg tcaacagatt gcttccacaa agattacagc tatagatgat ggtacaatac
     2461 caaactactg gggttctatg aatatagatg atgaaggaaa tccaactcaa aagaatatac
     2521 tgattgaaaa tggaattttg aagtcttata tgatagataa acttaatggt agacgaatgg
     2581 gaatgaatcc tacaggaagt tctcgtagac agaactacaa atttgcacca acttcaagga
     2641 tgactaacac ttatattgct gctggagagg atagaccaga agacattata aagtcaatac
     2701 cagatggact ttatgctaaa aaaatgggtg gtggttctgt aaatcctgta acaggagagt
     2761 ttaattttgc agtttcagaa ggttacttaa taaaaaatgg agaaatacaa gagccagtta
     2821 gaggtgctag tttaattgga aaaggtagcg atatacttat gaatatagat atggttggga
     2881 ataacgtaaa acaagctcag gggatgtgtg gctcatcatc aggaagtata ccaactaatg
     2941 taggccaacc tatgataagg gtaaaagaaa ttactgtagg tggaagatag gagggataat
     3001 tgtggaactt aaaaatttta aagatatatt atttaaaaag gctctaagtg aaggttttga
     3061 agagtgtgaa atatattatt atacaggaga aaatttaagt ataaatatat atgaaggaga
     3121 agttgagaaa tacaatcttg ataaatcttt tggtttatct tttagaggta aggtaaatgg
     3181 aaagataggt tattcttata cagaaatttt agatgataaa gctgttgata tgttgataaa
     3241 aaatgtaaaa gatggagtaa acactattga aaatgaagat gtacaattta tatatgaagg
     3301 agataagcac tataatgatg taaagactta ttctaaagag ctagaaaatt tagaggcaga
     3361 taagttgatt gatttagctt tagaaatgga aagagagact aaagcttatt cagataaggt
     3421 ggtaaattta tctaaatgta ctatatctta ttctgtatca agtaatggta tatctaatac
     3481 taaaggtctt aatttaagca ataaaactaa tatgcttata gggtttgtag ttcctatcat
     3541 agaagacaat ggacaaaaat atgatggaat aggatataat atagcaaatt ctattgaaga
     3601 aataaaacct tgtgagatag caaaacttgg tgttgataat gctttatcta aagtaggtgg
     3661 taagagtatt ccatcaggta aatataaaac tatattgctt aatgaagcta tggtatcttt
     3721 gcttagcaca ttttctggga tatttaatgc agattcagct caaaaaggat tatctttatt
     3781 gaaaaataga gaaggagaca tgatagcatc tcctatagtt acaatagttg atgacccact
     3841 tttagaaaat ggtatggcat ctactcattt tgatgatgaa ggagtagcta catttaaaaa
     3901 agaagttgtt ttaaatggaa agcttataac tttacttcat aacttaaaaa cggctaataa
     3961 ggcaggtgta aagactacag gaaatggaat aaaatcttca tattcatcac ctataagcat
     4021 atatccaact aatttttata tagagaaagg agataaatct ttagatgaaa ttataaagga
     4081 tatagatgaa gggttgatgg ttactagctt tgcaggactt cattcaggtg caaattcagt
     4141 aacaggagac ttttctttag ctgcaaaagg cttttatatc aaagaaggaa aaaaagtatt
     4201 tccagtagag caaattacag tagcaggaaa ctattttgac ctgttgaaag atattgaagt
     4261 tataggggaa gatttagaat ttccaatgag tagtataggt tcgccttcag ttgttattaa
     4321 agagctatca gtagctggaa aagatgaata attaaaaagt aattttgtgg ataaaaggtc
     4381 atgttaattt taattaatat ggctttttat tatttaagta gattttatta tctaagttat
     4441 atgaaaaagt tgtgatgcat acaattaata tcatacatta aatttaaagg aggagatatt
     4501 atgagtgatg cacattatga catgcacaag cgaaatggat attcaagtga tgagccatat
     4561 ccagagataa aagttttagg tccaaataag tactatgcag aattattgat ggatgactat
     4621 gccggagtat caagtgaatt tacgagtgta aatcaatatc tataccataa ttttgattta
     4681 gatgaaacgc atagagagtt aagtgaaatg tggataaata tatctataac agaaatgtta
     4741 catatggaga tattagcaaa gactataaga ttattgggtg gaaatcctgt atatagaggt
     4801 tctacaagtt cttgtggtgc atattggaat ggtggttttg tatgttatgg aaattctatt
     4861 tgtaacagac taaaactaga tttgcatttg gagcatgtgg ctattaataa ttattataaa
     4921 gatataagtc ttattgaaga cccttatata aaagctatat taaatagaat aatcctagat
     4981 gaaaaacttc atgtaagttt atttgaaaag gcaatagaga agtattgtaa ataaatttga
     5041 aaatttaatt attcatcatt ttattcatat atataaaata accatcatac attttcttga
     5101 agggagttgc tgttgatatg tattatgtat atgtgaatga tataaacata gcagtatatg
     5161 atttgaatcc gagtgctaaa aaaactgtac tttttataca tggttggccc ttaggacata
     5221 agatatttga atatcaaaca aatatattgc caaaactcgg atatagaaca gtttcaattg
     5281 atttaagagg ttttggtaaa tctgatgcaa catcaggagg atatacttat agccaattag
     5341 cagacgatat ttataaggtg gtacatgcta ttggattaaa agactttacc cttgttggtt
     5401 tttcaatggg aggagcaatc gtacttaggt atatgagttt atttaatgga tatggagtgt
     5461 caaaattggt attagctgca gctgctgcac catcatttgt acaaagacct ccagaatttc
     5521 catatggtat gacacgtgaa gatgtaaata aattgatagc acaagcatgt actgatagac
     5581 cagaaatggt aaccgatttt ggcgaaaaag tatttgcatc aaatccacca gaaagcttta
     5641 gaagatggtt taatgatatt ggttttagtg catcaggtat tggcactatt ggaactgctg
     5701 tatctttaag agatgaagaa ttatttaatg atttaaaatg tgtaagagtg cctactggta
     5761 tatttcatgg gaaattagat gaaatatgtc cgtatgaatt tgcagttttt atgaatgaaa
     5821 aaatagaaga ttcaatttta tatacttttg agtatagtgg tcatgctata ttctatgatg
     5881 agcttaaact atttaatcag gaatttttac agtttttaga ggaatagata tattttatta
     5941 tcacttagaa aaagagtaat attatgtgaa aagatttaag ctaaatttta tgatgttttt
     6001 tacactaaac aaatagggtt ttagaacttg taactaagtt ctgagaccct attattatat
     6061 attcctaaaa tttgttataa tttattcctt atcattctta aaaatgtatt aatatattaa
     6121 cgaatttata ttaaattttt gtttatattc caataatgtc aaactaaccg ttttagaaat
     6181 taaattatat gtgaaaaaag atagcttaca ttattttatt atggaaaata atagtaaatg
     6241 gtacaaaatt tgatttttaa gatttaaaac ttttttaaaa agtgctacaa tagaccaaat
     6301 tattactttc tatactcgtt aaacaggagg tgggactatg aaaaaacata atattttatt
     6361 tgtatcaact gatgacaaaa taaatataga tatatcaaaa caattggaaa atatatttgg
     6421 agaattttgt aatatagata acttggtata tgtaaatagg ataaatattg aactgtcaag
     6481 ctatgagctt gtagtatgtt cagacaatga tataaaggaa tatatacata ataatattga
     6541 taaaaatata cctattgtaa tagtacatcg tactataaat attgaaaata taaatcagat
     6601 aataagtata gaaaatgatt ctgatgttat ggttatagat gcttataaag agtcagcaga
     6661 tgaaactgcc aagataatta gaaaactagg attaattcat attaatctaa taccatatta
     6721 tccaggttgt gataagtcta aatgtgagat tggaattatt acgggaagca gaaacagcat
     6781 accacaaaac ataaagcaaa taattgacat aggtgataag gtaattgata taaatacagt
     6841 tatagaaatc tttacaaaac taaatatatc tatagataaa ttacatatta ttaaggaaaa
     6901 atacaatgaa gatacagtaa gtggatatag gtattataca actatgaata aaaccatgaa
     6961 gagttttttg gagattatag atgaaggtat tgcttctgtt gataaattag gaaaatttat
     7021 atattgcaac aaagtatttt ctaatctagt tggaatagac caaaatgaaa ttatatctaa
     7081 taactttatg gacttattta gtgataaggt tgtcaaaaaa atattttttc aagaagatga
     7141 agttaatgat gaagtagtaa atttaaataa caaaaaactg ataattaata aagtaaatgt
     7201 ttatgaaaat aatgaacgaa taaagagtat tataagcatt aaaaatataa gtgcaattca
     7261 agtgcttgag gacaagattc aaaataaact tcagtcaaaa ggttttgtat caaagtatac
     7321 atttgagagt gttgtaggtg agagtaaaat aataaaagaa aaaattaata ttgcaagaaa
     7381 aatagcaact acagactttt ctgtgttgat acttggagaa aatggaacag gaaaagaaat
     7441 atttgcccag gcaatacata atgaatctct aagaaaaaac aagccatttg ttgcagttaa
     7501 tctatcttct ttgtcagata cacttattga aagtgaactt tttggctatg atgaaggaag
     7561 ttttacagga gcaataaaag gtggaaaaat gggtatattt gaaagagcac acacaggaac
     7621 aattttttta gatgagatag gtgatatatc tttagatgta cagcagagat tgctaagagt
     7681 attacaggaa aaagaagtta tgagaatggg cggaagtaaa attataccaa tagatgttag
     7741 aattatagct gcaacaaata aagatttaaa gaaaaaaatt aaagaaggtt cctttagaga
     7801 agatttgtat tataggatta atgtattaca tatagagata cctagactta gagaacgtaa
     7861 ggaagatata cctcttataa gtaagtattt tttagatgaa ataaatagca acaaatgttt
     7921 tacagaggaa tcaatgcaag ctttaacatt gtatgaatgg ccaggaaatg taagagagct
     7981 taaaaattta atttattata ttgatactat agttgaggaa gatagagtag attatgagca
     8041 tttgccagaa caatttagat ttgaaaaaaa caatacttta gttaatgaaa atttcgattc
     8101 aattatctta gatttcaaac aatctaattt ctttgaggaa agtatatgta tattgaccag
     8161 tatagaaact tggaataata aaaatatatt attaggaaga aataaactgc aagagatttt
     8221 aaaagaaaaa gatatagttt taagtgtaga ccaaattaga aaaagaatag ataagttaaa
     8281 gagccatggt ttacttttat ctggagtaaa aaaacaaggt agttttataa ctgatgaagg
     8341 aaagaacttt atatcatatg taaaaattaa gggagtgata taggggacta aaaaggcaaa
     8401 acaccctaaa aaaatcccta taatggatag aaataaataa aattatcttt aagattaaga
     8461 taattcaaaa atattgaata aataatctaa gaagattatt tttaataaaa cagtagtaaa
     8521 tatcaacctt actactgttt ttattttttg caagatttat tatacaatta aaatttatat
     8581 tttggcacat caattgctac ataggaaaaa tataaaaaat tgtagaactt ggaggatgtt
     8641 gttatgaagt cagcttacaa aggatatgaa gcctatagtt atttagaaaa aggaaaagat
     8701 atttcatcta ttgagatgtg taaaggagat aaaagagtta aagaatacct tattgaatta
     8761 gatgatattc aagaagctaa ggttaaaagt atagtggatg aaaaaatatt tatatcatta
     8821 catgaacatc cagtattatt tccagaacac ctagaaagag atatatttga atataatagg
     8881 gaaggtaaac aaagatgtgc ttatgaagca ttatcaagag gatattatga tgctatattt
     8941 gataatttaa tggatggtgt atgtacgata acatcaaatt cagggtggaa atgggatgat
     9001 atattaatag atttaggcat gagattatgt gatttagctc atcaagattt tgtcataaag
     9061 tgtgaaaaag tagaagatat atacagagct aaaagtgaag gtaaagtagc attgatacca
     9121 acattagaag gtgcagcacc aatagaaaat gaacttgata gaatagatat actgtatgga
     9181 tttggtataa ggttaatggg tataacatac agcgaatcca atgcattagg ttcaggacta
     9241 aaagaagaga aagatggagg tcttacaaat tttgggaaaa aagctgttga aagaatgaat
     9301 aaaattggaa tggcaataga ttgctctcac gttggagttc aaactacact tgatgtaata
     9361 gaatatagca aggacccaat aatattaagc catgttgggg caagaagttt atggaacaca
     9421 aagagactaa ctccagatga agtattaata gcatgtgcta aaaaaggagg ggttataggt
     9481 gtagaagcag cacctcatac tacaataaca gagaagaata aagaacattc aatagagtca
     9541 tttatggaac attttgagta tattaaaaat ttagtaggaa tagaccatgt agcttttgga
     9601 ccagatacag tatatggcga ccatgtaaaa ttacactcag tactagctgc taaatttgat
     9661 ataaaaaaaa taacaggaac aaaagaacat cccaaagtag aatatgtaaa aggtcttgag
     9721 aacccaacag aaacttcaaa aaacattgtt agatatttag taaagtatgg atatagtgat
     9781 gaggaaatag agaaggtact aggtggaaat atacttagag tactaaaaca ggtttggaaa
     9841 tagatatatt agaaatatta ataaaatttg aggatttata gggaggtttt ataaatggat
     9901 tatttaaaat tagctaatca tccacttttg tggatagcga gtacagtagc agtatcaata
     9961 gtagtggtac aatcccttat atttgctata aaatcttgca aagttggtaa aacaatggga
    10021 ataacagata aacaaataaa gtcagctgtt aagagtagtg ctatttcagc tattgggcct
    10081 tcaatgacta tatttgctgg aatggtatcg cttatagtaa ctatgggtgg gcctatagca
    10141 tggatgagat tatcatttat aggttctgta atttttgaat caatgtcagc cggatttgga
    10201 acagatgcat taggtataac tttaggttct cctgaaatga caaaacttgc atttacaaat
    10261 gcagtttgga ctatgatatt aggttcttta ggatggataa tattcacatt actatttgga
    10321 cataaactag ataagataac caatgttata tctagtggca agaaatcatt tataccaata
    10381 atatctctag gagcaatgtt gggttcattt gcatatctaa atgctgatag ggtgttgcgt
    10441 tttgataatg gaacaattgc atgtataagt ggaatggcta taatggtaat attgtgtatg
    10501 cttgaaaaga agaaaaatat aaaatggtta agagagtggg ggcttacaat atcaatgttt
    10561 agcggaatga taatagcttc agtaatataa ggggggagtt ttatgagtaa agtaaaatct
    10621 atatacaatg aagaatattt accttttatg ataaggtatg gaagactaac actatctttg
    10681 gggattatag cagcattagt tcctggaata attttgagct ttggatttgg tattatgcct
    10741 ccaatatcag cattattagc aagtacaatg gcaattgtca gtatgagtgc tccaaattac
    10801 ataatagaac cagtttctta ttcaccaatc ttaggtatcc caggaactta tatgagtttt
    10861 ctttctggta atatatctaa tatgaggcta ccatgttcaa tagcagctca aaaggcagca
    10921 gaagttgaat caggtacaga agaaggctcg ataatatcta caataggaat agctgtatct
    10981 attttagtga atataagtat actaactata ggggtaattt taggaggaag cgtactatct
    11041 aaaattccag ctgaagtagt agaaaaatta aacttaattt taccagctct atttgggtca
    11101 gtttttggac aagtattttt acaagataaa aaattaggcc tagtagcaat tgtaatatct
    11161 gtattgacta ttatattatc aaagcaaggt ataataccac agtctctagt agttcttata
    11221 tgtgtatttg gtactatatt aattgcaaga gctatgtata aagataagct tagtgactaa
    11281 ttacaatgaa ataatagtga attttcaaat atgaaattta ataatttgat tttcaaatat
    11341 aaaaattaca ttaagattat ttaaaaattg gtttttaagg agggaatatt ttgatatatg
    11401 atttaatctt aaaaaatgga tttataatag atggtacagg taatcctggt ttttatggag
    11461 atatagcaat taaagacaac ttaatagcaa aaatagattc taagataaat agcaatacaa
    11521 ataaagagat tgattgttgt ggaaaagtta taacacctgg atttattgac cctcatgttc
    11581 atgaagaaat agtagcaata ctagatggta aatttgaaaa gtttctaaaa caaggagtta
    11641 caacaactat aaatggtaat tgtggacatt ctataacacc atattcttct gaaaatgtat
    11701 atgaatatat gtataaaaat ggtctattac ttgaagaaga aaaaaagtat cttatagata
    11761 aaaataaatt ttggaataat tttacagaat attgtgacct tatatctaaa agtggtattt
    11821 ctgtcaatat gggattcttg ttggggcatg ggactataag atggagtgtt atgggaggtt
    11881 ctaaggatag acctcctaca gaaaaggaaa aaaatgaaat tacagatata ataaatgatg
    11941 gaatgaaaag tggtgctttt ggaatatcta ctggattggc atatatacca agtaagtatg
    12001 ctgatattga tgaacttgtt gatattgcaa ggcagattaa agagcatgat ggtatatata
    12061 catctcatat tagagattac ataggaagat ataatgcagt aaaagaagcc atagaggttg
    12121 ggcagaaaag tggtgctagg gttcaagtat ctcacttaag tccagttgaa atagaagcat
    12181 ttgatgagat attaaaggct aggtataatg gtgttgaaat aatggttgac acagtaccaa
    12241 gaagttctgg gcattgtatg aagaaaaaaa gagtaattca atttattatg gcaatatcat
    12301 ctagtctatt tgaattaggt atagatgggg taatggatgc tcttagaaat gaagagggaa
    12361 gaacacttat attaaaagaa gcatttatac ttggagatag ggggagtata atacttctaa
    12421 acactaaaga tattaatatg gaaaagaaat caatacgtga aatagctaca caaaaaggaa
    12481 tagatgagga caagttatta ctcgatttac ttttagatgg agacgaagaa cttatctttt
    12541 gtttaggagg aatgtataga gctgatttcc cagataaact acatgatgat aaaataattg
    12601 ataatccatt tgttatggtt ggttcagatt gtttgtttag tgtgggtggg gatatgtcat
    12661 ggtttgaact acaaagaaga ggagcttttc caattttctt caatatgtac agaaagtctg
    12721 gtgttagatt ggaagagatt gtaagaagag ttacttcatt accagctagg caatttaaaa
    12781 taaaaaatag aggtattctt aaagaagggt tgatagctga tatagcagtt attgacatgg
    12841 ataactatag ttatcctagg tcagaagata ttgatttttc aaagccacaa ttactagcca
    12901 atggtgttga atatgtaatt gtaaatggaa aaattgcgtt ggaaaatgga gagattactg
    12961 agaataaatg tggtgaggta ttaaaaagat aaggttttaa aaataatagg atttagaaag
    13021 taaataaata tctaagtaag taaagacgaa gtttgagatt tatttaaata aaaatc
//
LOCUS       DAFKMX010000116        13049 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFKMX010000116 DAFKMX010000000
VERSION     DAFKMX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.116"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            82..2565
                     /gene="recQ"
                     /locus_tag="LZB08_002553"
     CDS             82..2565
                     /gene="recQ"
                     /locus_tag="LZB08_002553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBM7316045.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYAKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      2654..2765
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2907..4208
                     /gene="thiC"
                     /locus_tag="LZB08_002554"
     CDS             2907..4208
                     /gene="thiC"
                     /locus_tag="LZB08_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBM7316046.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            4262..4456
                     /gene="thiS"
                     /locus_tag="LZB08_002555"
     CDS             4262..4456
                     /gene="thiS"
                     /locus_tag="LZB08_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBM7316047.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            4449..5252
                     /gene="thiF"
                     /locus_tag="LZB08_002556"
     CDS             4449..5252
                     /gene="thiF"
                     /locus_tag="LZB08_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBM7316048.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            5315..6085
                     /locus_tag="LZB08_002557"
     CDS             5315..6085
                     /locus_tag="LZB08_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBM7316049.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            6102..7205
                     /gene="thiH"
                     /locus_tag="LZB08_002558"
     CDS             6102..7205
                     /gene="thiH"
                     /locus_tag="LZB08_002558"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBM7316050.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            7206..7895
                     /locus_tag="LZB08_002559"
     CDS             7206..7895
                     /locus_tag="LZB08_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBM7316051.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            8083..9477
                     /gene="yfcC"
                     /locus_tag="LZB08_002560"
     CDS             8083..9477
                     /gene="yfcC"
                     /locus_tag="LZB08_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBM7316052.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            9994..11361
                     /locus_tag="LZB08_002561"
     CDS             9994..11361
                     /locus_tag="LZB08_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBM7316053.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            11386..11994
                     /locus_tag="LZB08_002562"
     CDS             11386..11994
                     /locus_tag="LZB08_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBM7316054.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            12042..12413
                     /locus_tag="LZB08_002563"
     CDS             12042..12413
                     /locus_tag="LZB08_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBM7316055.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            12592..12930
                     /locus_tag="LZB08_002564"
     CDS             12592..12930
                     /locus_tag="LZB08_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBM7316056.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         5261 a         1237 c         2403 g         4148 t
ORIGIN      
        1 accacgattg tgaatataaa aataaaaatt tgaacataat caggaaagaa gtctaattaa
       61 tttaaattag gagagtaata tatgaataaa aaacttttag aaatactatc aaaatactat
      121 ggatatacaa gttttagaaa aggtcaggaa tctataataa attctatctt aagcgaaaaa
      181 gatgtgctag ctattatgcc aacaggaggg ggaaaatcaa tatgttatca aattccagct
      241 ttgattttag atggaatgac aatagtaatt tctccactta tatcacttat gaaagaccaa
      301 gtagatgcct taaagactat gggaattagt gctgctttta taaatagttc attaagcagc
      361 aaagaattta atgaaatatt aaataatatt agaaaaaata attataaaat actctatatt
      421 gctccagaga gattagatgc acaagagttt attgagctaa taaataataa caatgtaagt
      481 caagttgcga tagatgaagc acattgtgta tctcaatggg ggcatgattt taggttgagt
      541 tatagaagga tttctgattt tataaaaaat ttgcctaaaa gacctatagt tacagcattt
      601 actgcaacag cttcagaaga ggtaagaact gatataataa atttactgtg tttagaaaat
      661 ccagattatt atataactgg atttgataga gagaatttat caataaatat agtaaaatca
      721 tctagtaaaa ataagtatat tttagattat attcaaaatc ataaaaatga gtctggaatt
      781 atatatgttg caactagaaa ggaagttgaa aatatatata atggtttatc taagagaaat
      841 atatctgtgt caaaatatca tgcaggttta tctaaaaatg aaagaagtaa aaatcaagaa
      901 gattttataa atgataatgt ggatataatg gtagcaacaa atgcatttgg aatgggtatt
      961 gataaaccta atataagatg ggttatacat tacaatatgc ctcaaagtat agaaaactac
     1021 tatcaagaaa taggtagagc tggtagggat ggagaaaaaa gtgagtgtgt acttttattt
     1081 tctccaggag atgtacatat acaaaaatat ttaattgata ttggagttga aaatcctgaa
     1141 agaaaattat ttcagcataa gaagcttcaa tacatgattg acttagttta cagtaattca
     1201 tgctatagaa agacaatatt gagttatttt ggagaaaatt atatagacaa ttgcaataat
     1261 tgtagcaatt gtttagtaga gggtgaaata gtagataaaa ctatagatgc acaaaaagtc
     1321 atttcatgtg tagctagaat gaaaagaagt tatggtgtta ctactattgt agatgtgctt
     1381 agaggttcta agaataaaaa gattttacag cttggattaa atactttgtc tacatataac
     1441 attatgagag attattctag tgaagactta aagaattttg taaatacatt ggtttctcat
     1501 ggttttttag atttagttga gactctaggc aatggaaata gaggtagttt tcctactata
     1561 agattaaatg agatgtctat gaaagtttta aaaggtgaag tgaaagtaga gtttaaagaa
     1621 attgttatga caaaatcatt ggaagtagag gatgagttat ataatgcttt aagagaactt
     1681 agacattcaa ttgcaagtga agaaaggata gctccatata tggtatttgg agatggtaca
     1741 cttagagcca tgtctagtag ttatccaatt aataaagaag aaatgctaga tatctctgga
     1801 gtaggagaaa ttaaatatca aaaatatggt agagattttg aagcagtaat taaagaatat
     1861 gtcgaaaaaa ataatataaa taaaaaaata gatgaaaaaa atgtcgattc aaagaacgtt
     1921 aatagtgatt tcttagaggt taatacagat aaagttttat atgaaaaact gttagtcatt
     1981 agagaagaat atgcaacaaa ggaaaaatta cctgcatata tgattttagc caataaggct
     2041 ttgaaagaaa taagtggaag atatccactt gatgaagaac aattaaaaga tatttctggc
     2101 ataggtggag taaaatttga aaaatatgga gctagtattt tggcagaggt taataaatat
     2161 gtaaaagaaa ataatattaa tgttatttgg gagaaaaagg gcaaaaggaa gttaatatta
     2221 gatggagaaa gtagaaaaaa taatgaaata gcattagatt tgctaaatca aggaaaagat
     2281 atacaaagta tcagtcaaga aatagaagtt tcactgtcaa ccattattgg atatgtgcat
     2341 gactatgcaa aggatggaca tagtatatca tttgaattag gactagaaaa tttttataat
     2401 gaagataaaa agaagatgat tttagaagtt tgcgaaaaag ttggctatga taatttaaat
     2461 aacataaaaa gaaaacttcc agattcaata aagtatgaaa atataagagc tgtgatactt
     2521 gactcttatc ttgaaaactt aaaaaataat attatagcag agtaattttt aacttaataa
     2581 aaaataaaat ttgtattatt gtgttgaact aataattttg ctgtgttata ctgaatttgt
     2641 aaaaataatg aaaaattaga gctaggggcg ctagaattgg ctagctgaga gataaagccg
     2701 taacttacaa accctaataa cctgatcaag ataatgcttg cgaagggaag ctttttaata
     2761 taattttatt atataaatag gcaaattatt caatgttagg agaaaattgt ctaagtgtat
     2821 atacgattat aagagtgctt tccaaacgag gaaaagcact ttttttattt tcaaaaacta
     2881 taactttaat cttggaggat tgtaatatga actatacaac acaaatggat gctgctagaa
     2941 aaggcataat aactaaagaa atggaaatcg tttctcaaaa agaacaggta gatgtaaatg
     3001 aattaagaga attaatagca aatggacaag ttgttatacc agctaataaa aatcataaat
     3061 ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa aataaatgta aatctaggaa
     3121 tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa agttagagta gcaatagaca
     3181 tgaaagctga agctataatg gatttaagta attatggcaa aactagagaa tttagagaaa
     3241 aggttgttga aatgtcacct gcaatgatag gttcagtacc tatgtatgat gcagtaggat
     3301 atttggaaaa agaattaaaa gatataacag aagaagagtt tttaaatgta ataagacaac
     3361 atgctattga tggagtagac tttattacta tacatgcagg tcttacaaga agtgtttgcc
     3421 aaaaaataaa aaatcatgag agattgactc atatagtatc tcgtggaggt tctttattgt
     3481 ttgcatggat ggaattaaat aataaggaaa atcctattta tactaatttt gataaaatat
     3541 tggatatatg tgaagaatat gatgttacat tgagtttagg agatgcttgt agaccaggtt
     3601 gtataaagga ttctactgat ggtgttcaaa ttcaagaact tgtagtatta ggtgaactta
     3661 ctaaaagagc atgggaaaga aatgtacaag taatgataga aggtcctgga cacatggcta
     3721 ttgatgaaat agaagcaaat gtagtattag aaaagagatt atgccatgga gcaccttttt
     3781 atgttcttgg accattggtt acagatatag caccaggtta tgaccatata acaagtgcaa
     3841 taggaggagc tttagcatgt gctaaaggag tagatttttt atgttatgta actccagcag
     3901 aacatttaag attgcctaat ttagatgata tgaaagaggg tataatagct gcaaagatag
     3961 ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc tcgtgaatgg gataataaga
     4021 tgagtaaagc tagagctgat ttagactggt gtgagatgtt tagacttgca atagaccctg
     4081 aaaaagctaa gagatacaga gatgaatcaa ctcctactca tgaggatagt tgtactatgt
     4141 gtggaaaaat gtgctcaatg agaacagtta agaaaatatt aaacaatgag gaacttaatc
     4201 taatataatt tttggctagt atgactggaa taaaaagaag ctgaaaagag gaggtgattt
     4261 agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat ttgactgtta tagatttatt
     4321 aaataaatat aatttaaaaa gtgatagagt tgtagttgaa gtgaatttag agataattga
     4381 agaatctaac tacaatacat atgtattaaa agacgaggac attgtagagc ttattagctt
     4441 tattggaggt ggttaaaata aaaatatttg taaatgaatt attaacagat gttgaggaaa
     4501 atacaactgc ttataaagtt agagatagct ataacaataa atgtgatgta ttggtgttaa
     4561 atggatatcc aattaaaaca gatatgcccc tttgtgaaaa tgataaatta acacttatac
     4621 aaaaaggagt taagccatca cttgatgaat tagaaagctt gttgattgct agacatactc
     4681 caaatataca taataaatta aaaaaaggga aagttgctat attgggattg gggggacttg
     4741 gttccaatat agctatctct ttagctagaa taggagtggg agaactttta cttatagatt
     4801 atgatgttgt agaaccatct aatttaaata gacagcagta ttttatagat gatataggaa
     4861 aactaaaaac tgatgccatg atagaaaata taaaaaaaat aaatccattt ataaaattaa
     4921 ataaacgaga tattttttta aacaagaaca atatggatac tattaaggat tcagatttaa
     4981 taattgaggc ctttgatgat gcttcatgta aagcacaggt atgtaattat gtattaataa
     5041 atttaaaaga taaatattta atagcatcat ctggtatggc aggttactat gattcaaata
     5101 taataactac taagaagata aaagacaggt tttatatatg tggagatttt gtgaatgaag
     5161 ctaaatttgg tgaagggctt atggctccaa gggttgccat ctgcgcaaat catatggcaa
     5221 atttggcaac gcaaatactt atagatatgt aaatattaaa attagatata aaaaagattg
     5281 aatattaaaa aaatttaacg tggagggaaa taatatggat aaattgatac ttggtggaca
     5341 tgaatttaat agcagattat tggttggaac aggtaaatat ggttcaaata atatattgcc
     5401 tgaggtaata aaagaaagtg gaagtgaaat aataacaatg gcacttagaa gagtggattt
     5461 agacaataaa caagaaaaca tattaacgta tattccaaaa gagatgacaa tacttccaaa
     5521 tacatcagga gctactaatg cagaagaagc tgtaagaata gctagaatat ctagaaaaat
     5581 gggatgtgga gattttataa aaatagaagt aatatcagat actagatatt tacttcctga
     5641 taatgaagaa actataaaag ctacaaagat acttgctgat gaggggttta tagttcttcc
     5701 atatatgaca ccagaccttt atgctggaag aagacttata gaagctaatg cagcggcagt
     5761 tatgccactt ggagctccaa ttggttcaaa tagaggtctt caaatgaaag aaatgataag
     5821 aataatgata gatgagttgg atataccaat aatagtagat gcaggtattg gaaaaccatc
     5881 tcaagctatg gaggctatgg aaatgggagc tgatgcagta cttgtcaaca ctgctatagc
     5941 ttcagcagga gaccctgtac aaatggctag agcatttaaa ttagctgtag aaggtggaag
     6001 agaagcatat attgcaaaaa caggaaatgt gtcagaattt gcaaatgcat cttctccttt
     6061 aacaggtttt ttaggcaatt tatagaaagt ggtgattggt gatgagtttt tatgatgtaa
     6121 tagaaaaata tagggatttt gattttgatg gatatttaaa caatgttact gataatgatg
     6181 ttttaagaag tttatcaaaa gacaagcttg aagattttga tatattaaat ttgctttcta
     6241 aaactgcagt aaaacattta gaagatatgg cacaaaaagc acataaatta agtgttcaat
     6301 actttggtaa aacagtatgt ttatatacac caatgtatat agcaaattat tgtgttaatc
     6361 aatgtgttta ttgtagctat aatataaaga gtggaataaa aagaaaaaaa ttaactatgg
     6421 atgaaattag agaagaggga gaagcaattt caaaagaagg tttcaaacat ctacttgttt
     6481 tgactggaga aagtagtttt cactcaagtg tagaatatat aggtgaggct atagaaatat
     6541 taagagagaa atttccctct ataggtatag aagtttatcc aatggaagta gaagaatata
     6601 agtacattgt agataaaggt gttgaaggat taacagtata tcaagaaact tatgacgagg
     6661 aaatatataa gagagtacat ataaaaggcc caaaatcaaa ttataaattt agactagatg
     6721 ctcctgaaag aggggcaaaa gcaggtatga ggactttatc aataggtgca cttttagggc
     6781 taaatgattt tagaaaagaa acttttttta caatattgca tgggaaatac ttaaaaacga
     6841 aatatcctca tattgagctt tcatattcaa caccaagaat gagacctttt aaagggtgtt
     6901 ttgaggaatt ggtagatata agtgatactg atttagttca agctatggta tgtatgagat
     6961 tatttgaccc tcatgcagct ataaatatat ccactagaga aaatttagaa atgagaagtc
     7021 atataatacc tttaggagta actaaattga gtgcaggtgt atctactgat gtaggaggtc
     7081 attctcaaga tgaacatgat actgctcaat ttaagataaa tgatgaaagt actgttaaag
     7141 atgtggagaa aatgctaaac tcaattggtt atcagcatgt gtttaaagat tgggaaagat
     7201 tttaaatgta tctgataaca aacagaaaat tatgtagtga ggaacgatat ttagaagtta
     7261 ttaaagagtc tatattgagt ggtgtagaaa atataataat aagagaaaag gatttggaat
     7321 atcaagagct aaaaaaatta tatatgaaaa taaaaactaa aatcaattgt atagattttc
     7381 aagagcaaat atcagatgaa agtttgaaaa ctaatataaa tcaaaaagag tgtagaaata
     7441 aattcaaggt taattttata ataaatagta acattgaatt ttttgaaaaa gtggattgtc
     7501 aaggtatcca tcttcctttt aaattattct tgaatttaat tgaaaataag tataatttta
     7561 atgaaaataa aatactagga ttatctttac ataaagtaga ggaagtagat tatttagaaa
     7621 aattaataag aaatcaaaat ataaaaatag attacataac attatcacat atatatgaaa
     7681 ctaaatgtaa agaaggatta aatccaaaag gaattgagct tttaaaagaa gctaaaaaga
     7741 ttactgatat taaaataatt gcacttggag gaattttacc atctaatgtc aaagaaacat
     7801 taaaatactg tgatgatttt gctataatgt caactataat gaggtctaaa gatattaaaa
     7861 aaaccatatc aaattataat gaaaagttga attagtaaat atagtatatt gaatttttaa
     7921 cttaaataaa aataaaaaag ttatctttta aattttaaaa ggtagctttt ttattttata
     7981 tctttgctaa taaaaaattg taaaaaggga aaactttagt cagagagatt aaacaatatc
     8041 ctagaaagat ttaaaattta gtaaagtatg gaggacatta taatgactga cagtaaaaag
     8101 aaacgattta ttataccaca cacttttaca ataatatttt tactaatagt cttaatggca
     8161 atattgactt ggatagtacc aagtggtgaa tttagtagag aagatgtaga aggtagaatg
     8221 gtagttgtac cagggactta tcaaagtgta gaaagtaatc ctcaaggatt tgcagatgtt
     8281 tttaaagcac caatagaagg ctttatcgat tcagctgaag ttgtaggttt tgttttaata
     8341 gttggaggtg cttttggtat tgtaaatagg actggagcta tagaagcagg aatagcttcg
     8401 gttatctcta agtttaaggg aaaagaactt cttataattc caatatcaat gattttattt
     8461 ggacttggag gtacaacatt tgggatggca gaagagacat taccatttta tatgatattt
     8521 gttccactca tgacatcctt gggatatgat tctctaacag caatagcaat tgtatttata
     8581 ggagcagcag caggagcagc cgcttcaaca ataaatccat tttcagttgg tattgcacag
     8641 gcactagctc aacttgctcc tggttcagga gtaacttaca gagtagttat ttttatagca
     8701 tatatactaa taagtatagc atttgttatg tactatgcta agaaggtaaa ggaaaatcct
     8761 gaaagttcaa tagtatatga aatagataaa aataataaat caatacttaa tcaaacttca
     8821 acagaaataa aagaatttac ttttaaagaa gtatctgttc ttgctatatt tggaataggg
     8881 atggcaataa tgatttgggg tgttttaagt caaggatggt acattccaga aatatcaatg
     8941 tgttttgctg caataggtgt gctttctgga ttggttggta gattatctca aattgaaatc
     9001 tcagaaagtt ttatagatgg agcaaaagat ttagcatctg cagctcttgc tatagctttg
     9061 gctcgtggaa tcgtaataat tgctcaaaat ggatttataa tagatactat attaaattcc
     9121 gcagcagcct ttctaggtgg gcttccaaag gttatatttg tatatttatc atttttcttg
     9181 gaagctgcat tggcattttt aattccatca tcttcaggtc ttgcttcact tacagtacct
     9241 gtacttgctc ctcttggtga tttggttaat atatcggctc aagtgatagt aaccgcttat
     9301 caatttggtg tgggtcttac aaactttata actccaactt caggagtact gatgggagca
     9361 cttgcagtag cccatattcc attttcaaaa tgggtaagat ttataatgcc tatcttgttg
     9421 attttgacag ctgtatctct agtcttttta acaataggta tttatatagg gttttaatta
     9481 agtgtagttt tatttaagat atttagtaat ggtataaaat gataatatgt tttcaataaa
     9541 ttgccactaa tttaattagt ggtaatttgt tttttgtatt taaaaataaa tgtatttact
     9601 tgtattttga attaatatct acttagacat tgttttaatt gttatatttt catttaatta
     9661 agcattgatt tatcattttt tatatttcat ataacaatgc aaaaaataat ttttatatag
     9721 taaatgacaa aagtttacaa taaatgtata ttttcttact cattccttaa aattactcat
     9781 ttagtttaat aactctcatt tcatatgtaa agttggtaaa aaaattaaaa ttaaaagtaa
     9841 aaaactaaat aaattaatgt taatttgaaa tatatatgaa ggtttttgga gaatacttac
     9901 ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa tataaaggac tgaaataatt
     9961 aacagttatt aaatattagg agtgtaaaaa tttatgggat taaaagaatt tgattttatt
    10021 gaagagtcta ttgatatgtt gaggacaatg tcaccaacat tagaaactat atcagatgaa
    10081 atagaagagt attttgaaaa tatcttggat gaaaagaatc aagaatatat aaatgtaacc
    10141 tctagaataa aatcagaatc aagtttaaga gaaaagataa ttagaaatag atatttaaaa
    10201 aaatatggag aagcaagtaa tcttattcat aatgtttctg acttaatagg cctaagaatt
    10261 gagtgtagat ttattgagga tgaaaacaaa atatatagac ttttgagaag atattttaat
    10321 aaaacagatg acaaaataaa ctattataat aaagagaata agaatattaa attaaaacta
    10381 tctgagagac aacccaataa gcaaaaaaat ggatttgaaa tatataaaat agatggagtt
    10441 tttgcgtatt tagatagaga agtaaaattt gaacttcaaa ttaaatctct tgtaaatgtg
    10501 ttttggagtg aaatagaaca taaaataatt tataaaaaca atacatattt acttgctgat
    10561 aaatttataa aggatatgat ggactctata aagaataatc ttactatgat agataatcag
    10621 cttttaagta tttataaaaa ctttcattca gggaagtctt ttaatatgaa agtaagtaaa
    10681 aaagaaattg aaaaattatt tgcgaaatta gtctatgacg ctttttctga aaagatgaat
    10741 aaaagtatag gttttgttgt agattttaaa aaaccatgtg agactatatt gaactattct
    10801 tttaataaac aagatataag tgatgaagtt ttaggtaatt ttatgcttga tgagtttgca
    10861 agattaaatg aaattgtaaa caaggatata gattttaatg agcaaataga atttgaaaga
    10921 gaaccaacat ttgatgataa gttttgtaaa gacctaggaa atcattttag aagtagatta
    10981 aacacagaat ttccatggaa cctatttttt agaatattat ttgaaataga gccatataac
    11041 aataccaaag actttgaaaa ctttgtagag tttataaaag aaaatgtgct actagaagaa
    11101 aatagggagc aattattatg ccaatttgaa gaagatagca aacttataat tgaagatata
    11161 tatgattgta tatttaatag tataagtgag attgatagtg ttgaaatctt atataactat
    11221 aatcttgaaa aaataaactt tacatcaagt gaaatcataa attgtatatg tagagaatat
    11281 gagtgttatg gtgaatactt agaagaaaaa gaaaagctta tgaatacatt taaagaaaag
    11341 attattcaaa tctttgagta ataggttaat gctgaagtgg cgctaatgcg ccatttttca
    11401 aatatatatg aattaagtaa aattattttg gaggaggcga tttgtataga gaaaacaggt
    11461 gtaattttgg cagggggaag aaattctaga atgggtagag ataaagcctt tttagagtta
    11521 catgataagc tttttattga aattgcaata gaagcattta aaaactttga tgagttgata
    11581 ataatatcta acaatgaaga attatattct aaatacgata ttaaagttta taatgatata
    11641 gtaaaagatg ttggtccaat tggaggaata tatactgctc ttgaatatgc aaagtatgat
    11701 atagttacaa ttgcatgtga tatgccatat ctaaataatc aaatagtcga aagaatagct
    11761 aataaaatgg atgataaaag tgtgatttca gtgacaaatg gaaagttaca accattgtgt
    11821 tctggatata aaaagagtat aataaataaa gtatctttat gtataaggga aaatgattta
    11881 aagctcagaa gctttataga taaaatagat aaaagttata tatactttga tgaagaagat
    11941 ttatttttaa atgtaaacac tgttgatgag tatgaaaaat taactaaagt ttaattagct
    12001 atggttgtaa aatagagtag gatttatatg gaggagagtt tatgaattta cttaaatgcg
    12061 attcttgggc agtaatatta aataatagcg ataaagaaag taaagcatat aaaatattag
    12121 atgaacttaa aaggaatatg tacaaagttg ttgctataga tgaagagaaa aaacctattg
    12181 aaggaataga tgtgtatgaa tgtttaaaag atgttccaca caatattgat gttgtagcta
    12241 ttattgataa acaatctaag atggatgtga ttttagaaga agtagagctt ttggatatac
    12301 aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat gattaaaaag actaaagatt
    12361 taaaattgaa tatagaatat aatttaagtt tatatgatga gttgactaga taaaattata
    12421 tttcttaata ataaaaatat aattagttaa taaaacatat tattttatag tatagattgg
    12481 ttaataaaag tacagattgg ttaatgaaac attaaattac aatatttgtt aaaaaaattg
    12541 tgagttacat gatataatgt attaaatagg atactaagga gttgatatat tatgttaata
    12601 gtaactacag aaaaagtaga aggtaaaaag atatcaaagg ttttaggatt agtgagagga
    12661 agtacaataa gagcaaaaca tgttggaaaa gatataggag caagttttaa aaatcttgtt
    12721 ggaggagaac ttactggata taatgaaatg ctcactgaag caagacaaat tgccataggt
    12781 agaatggttg aagatgcaga agctaaaggt gcaaacgcag taatagcatt tagactgtct
    12841 tcagcttcag ttatgcaagg ggcagcagaa atgcttgctt atggaacagc agttgtttta
    12901 gaagatgata atagtattct tgaaaaataa aatataaata gttaaaaaga ggcatctttt
    12961 aaaaaatttt atggatgcct ttttttattt gaaaaaacta atttgagaag aaattttaac
    13021 tattatgaca ataaaattag ggtgatatt
//
LOCUS       DAFKMX010000117        12960 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFKMX010000117 DAFKMX010000000
VERSION     DAFKMX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.117"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            164..1537
                     /locus_tag="LZB08_002565"
     CDS             164..1537
                     /locus_tag="LZB08_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316057.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1793..2860
                     /locus_tag="LZB08_002566"
     CDS             1793..2860
                     /locus_tag="LZB08_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBM7316058.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2958..3266
                     /locus_tag="LZB08_002567"
     CDS             2958..3266
                     /locus_tag="LZB08_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBM7316059.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTIIGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3259..4689
                     /locus_tag="LZB08_002568"
     CDS             3259..4689
                     /locus_tag="LZB08_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBM7316060.1"
                     /translation="MNNSTSMKDMSYQAVMGKNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4767..5141
                     /locus_tag="LZB08_002569"
     CDS             4767..5141
                     /locus_tag="LZB08_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBM7316061.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5135..7339
                     /locus_tag="LZB08_002570"
     CDS             5135..7339
                     /locus_tag="LZB08_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBM7316062.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7339..8748
                     /locus_tag="LZB08_002571"
     CDS             7339..8748
                     /locus_tag="LZB08_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBM7316063.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8733..9797
                     /locus_tag="LZB08_002572"
     CDS             8733..9797
                     /locus_tag="LZB08_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBM7316064.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFRINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9827..11248
                     /gene="nhaC"
                     /locus_tag="LZB08_002573"
     CDS             9827..11248
                     /gene="nhaC"
                     /locus_tag="LZB08_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBM7316065.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11677..12141
                     /locus_tag="LZB08_002574"
     CDS             11677..12141
                     /locus_tag="LZB08_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBM7316066.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12439..12801
                     /locus_tag="LZB08_002575"
     CDS             12439..12801
                     /locus_tag="LZB08_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7316067.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
BASE COUNT         5106 a         1413 c         2513 g         3928 t
ORIGIN      
        1 aaattttaaa tatacttaaa aatatattaa tataatgtac ataaaaaata tcatcaatat
       61 atgataagat aaatacaata gcaacatttt attaattttt tgggttttta tatatttagg
      121 tactggggtg atatagttga accaagagat attttttaaa ataatgaatg aaatattgga
      181 aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa tctacaataa
      241 ttctgtacaa aaaatggaaa aaatgaacag acacgatatt ttagccaatt cattttttga
      301 tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg aaaaaaggga
      361 agttatcaaa gacaatattc aaaaatatct agataaaaat ggaaaagaga taacagcaat
      421 taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg aaatatcaaa
      481 gaatatgaca acgatagaaa atttactaaa cagaaattat aaaaaaaatt tagtcaataa
      541 agttagtagt agacaaaaaa agggttatga atttgatgat attttagggg aaagtaattt
      601 gattaaagaa gcaatagaaa aatctaagaa agcttgcaag tctgatgctt cagtatttat
      661 atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcact atggaagtaa
      721 taggagaaac tatccattta tagcacaaaa ttgtgcagct ttgccggaat cactattcga
      781 aggtatcctt tttggaacta gcaagggagg atttacggga gcaatagata ggccaggctt
      841 atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca tgcctattat
      901 gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg ttggaggaac
      961 taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc caaaggtaat
     1021 tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa ttcatataaa
     1081 aattcctcct cttagagata gaaaagagga catacctcta ttgtgcaaaa aatttatttc
     1141 aaagtataat aaaaagttaa ataaaaaagt ggtaggaatt tcagatgaag ctattaaaat
     1201 tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa tttattgtac
     1261 tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc taaacgacta
     1321 ttacaatctt aaagagaata aagagagtaa caattattct ttaaacgatt tggaggacaa
     1381 aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat cattagaaaa
     1441 ggcatcgtac aatataacta aagctgcatc aattttaggt atgaataggc aaaatttaca
     1501 gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa aaaaacaaaa
     1561 tcaaagtaaa ataaaaaagc aaaatagagg agcaaaatta ttataaaaga gcaaaatatt
     1621 ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa atacataata
     1681 aaataattat agtttcaacg ttccaataaa tatttgcttt aattttaaag ttggcacgat
     1741 ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat atatgagaaa
     1801 agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta atatgattct
     1861 aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag ggaaaagtat
     1921 gtatgatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca ataaaaatta
     1981 tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg attcatttac
     2041 taaaaaggct tttgataaaa taattttctt attaaataga aaaataaatg ttatatcaac
     2101 agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa aaatggatga
     2161 agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag gatttgtatt
     2221 agatttacta gtattggcac tttcaggtac ttgtgaagaa gttactagta taaaagctaa
     2281 gagagtaaat gatttatctc catttggaaa gtctgttatg gtggaacaag gtgttggagt
     2341 aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc atgtaggttt
     2401 tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata agatagaaca
     2461 aacaaaagag ccaataatga ctacagtaga tagaaaatct aagtacggag aggcattggc
     2521 tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag aagttttaat
     2581 tgaaatggag catccacaac agataatacc agaagcagaa ggtattaaaa caggagacta
     2641 tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa tacctggagg
     2701 agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg ctagaccagg
     2761 tcttaaaact atgttagata taccagtacc tagagcaata atgggagata taagaaatca
     2821 gatagaagtt gaattggaag aagaatcaaa ggctaattag taagataaca gttagctaat
     2881 tggtaaaata atgtttaatt taaaataaca gtaaattaat tagtaaggca atagtcaatt
     2941 aataagggga tgaaaaaatg gatgctaaaa ttaatgattg ggttattata cacaatatag
     3001 tattgactcc agaagaaaga gctccacaag ttccagagga tactaagaaa gtatccttag
     3061 aaatgtgggt taaaggtttt atacaaagtg atgcttctat aggcgactta gtagaagtta
     3121 aaacaattat aggtagactt gtaaaaggta atttacttaa agtaaatcca tattatactc
     3181 atgattatgg taaatgtata ccagagttac tccaaatagg aatccaagct aaagaaatat
     3241 tatttggagg tgtatataat gaataattca acatcaatga aagatatgag ttatcaagct
     3301 gttatgggaa aaaataacga aataatgaaa aatgcaatcg gattggatta ttcttctttt
     3361 gaacaagagg gaatagggtt tgattatgag aaaatgatga gtgaaacagg atatacatta
     3421 caagatattg aagcaattca atcacagtat gctgttggaa atactcctct aatagagctt
     3481 aaaaatttga ctaaattagc tagaaaatgt gctaaagaag gtaaaggagc tagaatattt
     3541 gtaaaagatg aagcaatgaa tgcatctggt agttttaaag caagaagagc agctactgca
     3601 gtatatcatg ccaaacaaat gggatataaa ggtgtaatag cagctaccag tggtaactat
     3661 ggtgcagctg tagcatcaca agcggcaatg cagggattaa aatgtataat agttcaagaa
     3721 tgttatgatt ctaatggtgt aggtcaacct gaaattatag aaaaagcacg aaaatgtgag
     3781 gcatatggag cagaagttgt tcagttaact gttggaccag agttatttta tacgttctta
     3841 gttttattag aagaaacagg ctattttaac gcatcattat attcaccatt tggaattgca
     3901 ggtgtagaaa ctttaggtta tgagttagct atccaattta gagaaaagta taaaaaagac
     3961 cctgatatag ttgtttgtac aaatgcaggt ggtggtaatc ttactggaac tgcaagagga
     4021 ttgataaaag ctggttctat taatactaaa gtagtaggag caagtgtaga tttaaaagga
     4081 ttacacatgg ctagtgataa tcaatttaat aaaaaatcat ttacaacagg acatacaggt
     4141 tttgggatac catactgtac atggcctgat aggtcagatg tacctaggtc tgcagctaga
     4201 ccacttagat atatggatag atatgtatta gtaaaacaag gagaagtttt ttatacaact
     4261 gaattattgg cacagctaga aggtatagaa agaggtccag caggaaatac atctttagca
     4321 gcagcattct ctttagctca agagctagat gaggataaag ctatagtggt acaagaaaca
     4381 gaatatacgg gtgcaggtaa acatatatgt ccacaactta cctttgcaag agaaaatgga
     4441 atagatataa aatttggaaa tccaagagaa gaaattgcag gagtaaactt aatattacca
     4501 gaaagaccag aattattgaa atgtgttgat attgatatga ataaaataag aaagtcattt
     4561 ataaaaaatt gtatatctaa taatcacatt gataatattg ataagctaag taatcgagat
     4621 atagaatttt taatggaaga agtaaaaagt tcaagagatt ttgttgttga tgtattaaat
     4681 aatttatagt aattattgtt ttttaattta atataaaata aaaattcaga aaattcgatt
     4741 aaatcaatac gaatggagga atgttcatga aaaaaagaga ggatgatttt gaagttagaa
     4801 gaaaacatct tcaagaactg agtgatgatg agttaaaaga aaggttttgg agtttagcta
     4861 cccagatagt agaaccaatg atagaacttg gtaaaaagaa tacaacacca tcaatagaaa
     4921 ggtctgtctt gttgagaatg ggattttctt ctcttgaagt aaaacctatt ttagaagggg
     4981 ttatggaaag aggtcttata ggtaaaggag cagggcatgt agtttataaa ttggctaaat
     5041 ctaaaaatat aacggttaga gaagcaggtt tgttattagt taagggtgaa tattgggatg
     5101 aagttacatg tttatttaga gaaggggtag aatcatgtta aaggaaaata aaaaattgga
     5161 tatagactat atattaaaag atttggataa atataaacca aaacgaagag gttgggtttg
     5221 gagagaacac ttggaaaatc ttgaaatggg accttttaaa tataaagact gtacaaaacc
     5281 tttaaagaag agtgtagggc taccatcatc taagtatttc aataatatag accctcaacc
     5341 aagtccagtt attactactg aaatagcttc tggtagattt gaggatgata taagaagaat
     5401 gagaatggca gctcatcatg gagctgacca tctaatggtt atcagaactg ctggacaatc
     5461 acattttgat gggcttatag aaggaactcc acaaggtata ggtggagttc caataacaag
     5521 aaaacaagta agagctcaaa ggaaggctct tgattttata gaagaagaag ttggtagacc
     5581 tattaattac cattcatatg taagtggtgt tgctggtcct gaagtagcag taatgtttgc
     5641 tgaggaagga gtcaatggag cacatcaaga cccacaatat aatgttctgt ataggaatat
     5701 aaacatggta aggtcttttg tagatgcttg tgaggcaaag aaaataatgg cttttgctaa
     5761 tatagctcaa attgatggag cacataatgc taatgctact gctagagaag catggaaggt
     5821 aatgcctgaa cttatggttc aacacgcttt aaactctata ttctcagaaa aaataggtat
     5881 agataaaagt aatatatgct tatcaacagt tccaccaaca gcacctccag caccatgttt
     5941 aaaaatagac ttaccttacg ctgttgcact tagagagtta tttagtgatt ataagatgag
     6001 agctcaaatg aatactaaat atatggaatc atcaactaga gaagcaacag ttactcatgt
     6061 attaaactta ttaacatcag tattaactag agctgacata caatcaacta taacacctga
     6121 tgaaggtaga aatgtacctt ggcatatata caatatagaa gcatgtgata cagcaaagca
     6181 agctttagtt ggtatggatg ggcttatgga tatgatagaa cttaaagatg ttggtgaatt
     6241 aagagacaaa gcaagagaac ttaaagaaag agctgtatta tatatggaag aaatatctga
     6301 agttggtgga tattttgaat ctgtggaaca aggatttttt gttgattctg gaaattatcc
     6361 agaaagaaat ggtgatggaa tatctagaaa aataaaaggt ggagttggtg aaggtactgt
     6421 ttatgaaaga gaagaagatt atttagcacc tgtaactgct cactttggat acaataatgt
     6481 agctcaatat gatgaaaatg ctatagataa tccatcaatg ttaatagatg gatgtacatt
     6541 tgaaaatcca gacaaaataa tatatattga tgagcttgac gattttgata atgtagaaaa
     6601 tagactaaaa gaatcatatg agtacagaaa tggaactaaa attaagcctg aaatggaatg
     6661 gtgtgcagat ggaattgtaa tgataacaat gatgcttcct actgataaaa gaacagcaga
     6721 gtttgcagct ttggaatttg tcaaaaaaat gaacttacaa gaaattgagg tcataagtag
     6781 agaagttatg catgaatctg aagggactag aatagaagtg aaaggaagag ttccgtttga
     6841 tatagattta aacaatttag taataccaga agaacctaag gtactaactg atgaagaaat
     6901 aagagcagat atagaagaaa aacctatgaa aatagtttca gctacggttg gagaagatga
     6961 acattcagtt ggtttaagag aaataataga tataaaacat ggaggtattg aaaaatatgg
     7021 catagaatgc cactatctag ggacttctgt acctgtagaa aaattggtag atgcagcgat
     7081 tgaactgaat gcagatgcaa tacttgcatc tacaataata agtcatgatg atattcacta
     7141 taaaaacatg aaaaaattac acgattattg tgtagaaaaa ggtataagag ataaagtaat
     7201 gattgcgtgt ggaggaacac aagtaacacc tgaaatagct gttgaacaag gcatagatgc
     7261 tggatttgga agaaacagca aaggcataca tgtagccact ttcttagttg aaaaaagaag
     7321 agaaatgagt aataaataat ggaaaatagc aaagtttctt taaaaataga tgtgttggta
     7381 gctgaaattg gaagtactac tacagtggta aacgctttcc acgatattaa tactaacaat
     7441 cctatatttt tgggtcaagg tcaagctcca acaacagtgt ttgaaggtgg cgatgtaaga
     7501 aatggcttat caggtgctat taaagattta gctaataaat tatctgtaga tgatatagag
     7561 tataatgata tgtttgcaac ttctagtgca gctggtggat taaaaatgac tgttcatgga
     7621 ttagtctatg atatgacagt aaaagcagct aaagaagcag ctttaggggc tggagctgtt
     7681 attcgtcaaa taacatcagg tagaattaaa agaactgatt taaataaaat aaaagaaata
     7741 aacccaaaca taattttaat agctggtgga gtcgattatg gagaaagaga tacagctatt
     7801 tataatgcag aaatgatagc ttctatgaat ctaggcatac cagttatata tgctggtaat
     7861 gttgaaaatc aagaggaaat aaggttaata tttgaagata ctaattataa gctttatata
     7921 actgaaaatg tataccctaa aatagattta ttgaatatag aacctacaag aagaatcatt
     7981 caaagtgtat ttgaagaaca cataactaca gcaccaggaa tgaaatacat taaagaaatg
     8041 gttaatgaaa atattactcc aaccccagga gctgtaatgg aagcttctaa acttttatat
     8101 aaaaatattg gagatttatt aactctggat gtaggtggag caactactga tgtacattct
     8161 gttacagatg gaagtgacta tattaataaa attttggtta atcctgaacc tactgcaaag
     8221 agaagtgttg agggggattt aggtgtatat gtaaatatga agaatatagt agaagttata
     8281 ggtaaagaaa acttacaaag tgaactatct atagatattg atgcagtaat agagaattat
     8341 cctccaatac caaaatcaaa agaagaaata ttatttgttg agaggttgac aaaagaagct
     8401 gttgttaaag ctatgctaag acattcagga aaaataagaa atatctatgg aacaactgga
     8461 aaagtcaaaa ttgcagaagg gaaagattta acagaagtaa gatatatagt tggtacaggg
     8521 ggagcactta ctaggctccc tagtagaatt gaaatattag ataatatgct caaatacaat
     8581 aaaaataatg aattactatt tccaaaggaa aaaacaaaga tattaataga taatgactat
     8641 attatggcat ctatgggtgt tttatctaaa aaatatgaag aagcatctct taagctcctg
     8701 ttaaaaagtt tgaattttga ggaggaaaga ttatgtatcc taggttagaa atagatatag
     8761 aaaagttaaa gcataatgca aaattaattt cgcagatgtg tcatgaaaga aatattaaga
     8821 tatcttttgt tactaaatca ttttgtgcac aaaaagaaat agtagaagaa atatgtagtg
     8881 aaggaataga ccatatagca gattcaagaa tacaaaactt aaaaaattta caagatataa
     8941 atctacctaa gatattgatt agaatcccaa tgttaagtga attagaagaa gttatcaatt
     9001 attgcgatat tagttttaac tcagaattag gaacaataaa gaaattaaat gaattatgtg
     9061 aaagtaaaaa tattattcat aagatagttc ttatgtttga cttaggcgat ttacgagaag
     9121 gatactttta tgaagaagac ttttttagta atgtaagaga gatagtaaga ttaaaaaata
     9181 ttgaaataat tggtatagct acaaacttaa cctgttatgg agctattata ccatctaggg
     9241 aaaatatagg cagactggta agtatagcaa aaagaatgga agaaaaattt agaataaatc
     9301 tagaaatagt atctggaggt aattcaagtt cgattcatct gttgataaat aataatatgc
     9361 cagaaggtat tactaatttg agaataggcg agtccattct acttggtaga gaaacggcat
     9421 acggtgaaaa tataaatgga acatatcaag atgcatttaa attaatatgc caagtagttg
     9481 aatgtaaaga aaaaccatca gttcctattg gagaaatagg agttgatgcc tttagaaata
     9541 aacctgttta tgaagataag ggaatcttaa aaagggcaat catagctata ggtaaacaag
     9601 atataaatat tgactcttta attccaatag acactgatat aaaaatatta ggggcaagtt
     9661 cagaccacat gatactagat gtaagtaaca caaaatatga ttataaatta ggtgacaatt
     9721 tagaattttt attaacttat gggggtatca tgtcatctag tacaagcaaa tatgtttcaa
     9781 aaaaaattat agtgtagtgt ttaaataatt aggaggaaac tattttatga aagagcgaga
     9841 atgtagaaaa gcaagtctgt ttgatgcaat gataccaatt acttgtttga tattattttt
     9901 aagtctagga gtattagtgt atggttcatc acctcatgta ccactaatag gagcagcagc
     9961 agtagcagga cttgtggctg tatatagact tggatttaaa tggaaagagt tagaattaag
    10021 tatgtttaat agtataaaga tggcaatgca agcaatctta atcataatta taataggcgt
    10081 acttatagga acttggatag taagtggagt agttccatgt atgatttact ggggactaaa
    10141 aattttatca ccaaatattt ttttagtagc atcaacatta gtctgtgcaa tagtatctct
    10201 tgcgacaggt tcatcttgga gcacaatggg tacagtagga gtagctcttt taggaattgg
    10261 tcaaagttta ggtatgccaa ttggattaat cgtaggttct ataatatctg gagcttactt
    10321 tggagataaa ctatcacctt tatctgaaac gactaactta gctccagcaa tggcaggaac
    10381 agatttgttt acacatataa aatatatgct atattcgact ataccatcat tattaatttg
    10441 tttagtaatt tatggtgtaa ttggaatgaa gtattcaggt caagcactag atataaaaca
    10501 aatagaatta attaggtcta cattagattc tacatttaat actttatcac cagtattatt
    10561 attagctcca gcaatagtaa ttggattggt tgtatttaaa gttcctgcta taccagggct
    10621 tatagtaggt gctgtattgg gagtattgtt tgcagtagtt tttcaaggag aatctatgaa
    10681 tacgatatta gatgcagcac aaaatggata tgtttcgtca acacaaatac aggaggtaga
    10741 tgctttatta tctaaaggtg ggattatgaa tatgatgtcc actgtatcct taacaatatg
    10801 cgctttatct cttggaggta ttttagaaaa aacaggtatg cttgaagtag tagcatcttc
    10861 tttactaaga ttagcaaagg gtgtatttgg aacagtatta tgtactatgg taacttgtac
    10921 aattaccaat ataatagcag gtgaacaata tctatctata gttataccag gaagaatgta
    10981 taataaggaa tataaaaaga gaggtataca tcctaagatg ttgtcgagag ctttagaaga
    11041 tagtggaact ttaacttctc cattagtacc atggaataca tgcggggcgt atataacagc
    11101 aacattaggt gtatctgcac taacatatgg tccatacgct ttattaaata ttataaatcc
    11161 aatagtatct ttagtattaa ttgcatgtaa gtttaaaata gcaagaatag aagatgaacc
    11221 tgaaacttgt ttaaattttg atgtttgata atagataact tatgtaagaa cgtaggaact
    11281 aggggggtag cctgtgggga attagtaaga catattttag tgtgcgaaat tctataaatc
    11341 agtaatcttg tgactttagt catgggaggt tcagattagt aaatttatag ttttatatta
    11401 gtctatattt tgatatttta attgtttgta taaatttttt atagaaaaag atttctttta
    11461 gacttgccta aaaattaggc aagtctttta agtatgttct gattataaga gcactgtgtg
    11521 acaagaatga tgagattatt tttgctatac tttcgatata agaaatggaa ggaggttatg
    11581 ttttatattc atatgaaata attagttaca cagtaaattt tcgtataatt tggtataaaa
    11641 agattagaaa tgaaaataaa gtaatggagg aataaaatga ctagaatatc caatataaca
    11701 atagctgaac aatctgagta ctgttttttg gcaatacgaa aaacaattga ctttatggta
    11761 gagttttcag agttttcaaa acaaagtttt gaaaaaattt ctaaatatct ggaggagaga
    11821 ggaattttat cttcaggagc accgattgta tgttttcata acatggattt agcaaaattg
    11881 gatgttgaag tagggtttcc tgtagccact tatatagatg ggaaaaatga gattctacaa
    11941 aggattgttc ctgcacagaa aataataact gcaattgatt taggacctta tgaattacaa
    12001 gaccctactt tagaagactt attttcatgg gcaaatagca atggatataa acttcatggc
    12061 gatatatatt accagtattt aaatgatatc aatcgtccag ctagtgaata tcttacgaaa
    12121 atgatgttac ccattatata gaaagttaaa gattatcgtt tgttagttga taattagaag
    12181 aacatttact gctactgcat aaattttatc ctaaataata tcaaggtgat ttaatttatg
    12241 tattctaatg taactgtaga taatcaattt ataactaact tagtgtaatt tagatttaat
    12301 ttattattct aatgtaaccc caatatattg ttaaggtcta cttaagatgc tcaaaaggct
    12361 atgcttaatt tattgttata taatttgtag agtggtataa tttagaggta atattatata
    12421 gatatatagg gggaaaatat gaaaatatat gatatattaa cacgttttta tattcaagat
    12481 atagaaaaag ccataccttt ttatgagaat cttttaaaag aaaaatgttc tttgcgtttt
    12541 tcctataaag aagttgggtt ggaattagcg caaataggaa atgttttact attatctggt
    12601 tctgatgatg ctcttaaacc atttatagaa acaaaatcta cttttatggt tgattctgtt
    12661 gatgaatgga gaagttatct attggataat ggagcagttg tagtaagaga taagaaaaaa
    12721 gttcctacag gatataatat gacattgaga catccagatg gtacaattat agaatatgtt
    12781 caacatacaa agcaggatta gttaaaaata gattttacta ctgagcaact aattagaatc
    12841 tattcttaga cgtacaattt attatttttg gtattaatag attataagtc aaattataat
    12901 atttcaaaat agaatagaaa tttatttaca taaaagagag tgtttaaaaa tgaacttttt
//
LOCUS       DAFKMX010000118        12748 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFKMX010000118 DAFKMX010000000
VERSION     DAFKMX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.118"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..1962)
                     /gene="recJ"
                     /locus_tag="LZB08_002576"
     CDS             complement(<1..1962)
                     /gene="recJ"
                     /locus_tag="LZB08_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBM7316068.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNT"
     gene            complement(1975..3639)
                     /locus_tag="LZB08_002577"
     CDS             complement(1975..3639)
                     /locus_tag="LZB08_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBM7316069.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3937..4434)
                     /locus_tag="LZB08_002578"
     CDS             complement(3937..4434)
                     /locus_tag="LZB08_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBM7316070.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4463..5842)
                     /gene="scfB"
                     /locus_tag="LZB08_002579"
     CDS             complement(4463..5842)
                     /gene="scfB"
                     /locus_tag="LZB08_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBM7316071.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNSDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6147..6293)
                     /locus_tag="LZB08_002580"
     CDS             complement(6147..6293)
                     /locus_tag="LZB08_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBM7316072.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6323..6901)
                     /locus_tag="LZB08_002581"
     CDS             complement(6323..6901)
                     /locus_tag="LZB08_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBM7316073.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7269..8699)
                     /locus_tag="LZB08_002582"
     CDS             complement(7269..8699)
                     /locus_tag="LZB08_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBM7316074.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            9043..9708
                     /locus_tag="LZB08_002583"
     CDS             9043..9708
                     /locus_tag="LZB08_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316075.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(10214..>12748)
                     /locus_tag="LZB08_002584"
     CDS             complement(10214..>12748)
                     /locus_tag="LZB08_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7316076.1"
                     /translation="TEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVE
                     KYNVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNP
                     IYKNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDF
                     NFSKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKS
                     ISKELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFE
                     KRQDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRK
                     IKVENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDML
                     DEKKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDY
                     NSERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGI
                     SIDKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIAL
                     EYAKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIK
                     AEALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQM
                     QLNLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKE
                     GISIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKING
                     MDIVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
BASE COUNT         4218 a         2182 c         1221 g         5127 t
ORIGIN      
        1 agtatttttg ctatcatctt caataatatt gtaatttatg ttaaaatcca aatctataag
       61 tgatatatct gacattgctc tataaaatcc atttatagaa ttagatatta ttagtgtatc
      121 tttttcaaaa taatcaaata tatttatatc tgtatttcca tcaaataatt caaaatcaga
      181 cacagatata ttatacaggc tttgagtatc ttttggtatt atttttgaca ttaatttcaa
      241 tgaaagtttg tcattacttg cacttttagg tcgagccaat cgtatatctt taagtaaaaa
      301 ttgtactttc ctatttccca tatagttatt ttcatctaat tggaatagta catctacttt
      361 gtctcctact ttatacattg attttaaatg actcatatta aatcctatac attcatagct
      421 tttctctttt tctatactta atttcagatg ttgttgattc ttacctataa ctttcaaatc
      481 ttttaaaata taatttctaa ctataaatct aggattagga ttatttaatc caaagggttc
      541 taacttatgt aattcttcta caagatttaa atttattaca ttttcttgta attcaaactc
      601 gacatttaca ttctctatca tatcatcttc agttaaatta taatctgcaa atttatttat
      661 ttcatttgct aaaatttcta cattgtctga atccaaggat aaccctgctg cttgttcatg
      721 accaccaaac ttagtcatca agtctttgca ctttatgaga gcttcaaata tattaaatcc
      781 ttttattgac ctcgctgaac cagtagcctc cccattttct atacctagaa gaattgttgg
      841 tttgtagtat ttttcagtta actttgatgc tacaattcct attattccat gttgccaacc
      901 ctctttagct aaaaccaaca ctttgtcatc attgtatctg ctattagact ttagcatttc
      961 ttcagcttca tgatacatct tagcttctat catctgtctt tcattatttt tttcttccaa
     1021 tatactagct atttctactg cctcttcttg tgattgagta gtaaatagct ctactcctaa
     1081 gtaagaatat cccaatcgac ctgaagcatt tatcctagga cctattgcaa aacctatatg
     1141 agaagaacct atcttatcac tttctacacc acaaactttt ataagctccc ttaatccaat
     1201 atttttgcct tctttcattg attttaaacc attttttaca attattctat tttcatctat
     1261 aagtggaaca atatcacaaa tagttgctag tgttactatt tctaaataat tatacatact
     1321 agttttaaat tcttcttttg gagtcaaggc ttgaatcatt ttaaatgcta caccacatcc
     1381 acatagtgaa tcaaatggat aattacaatc ttcttgtttt ggatttatta ctgcaaaggc
     1441 tgatggaatt tcactttgac attcatgatg gtctgtaatt ataacatcta tgcccaaatc
     1501 attcgctgta ttaacctcag acactgatgt aattccacag tcaacactta taatcaagtc
     1561 acatccccta gagcttataa gcttaatagc atcttcattt ataccatacc cttcttctaa
     1621 tctattagga atatagtaat caacattgta acctatactc ttaaaatata agcacaagat
     1681 agatgtagat gatacaccat caacatcata atctccgtaa atccaaattt tttcgttctt
     1741 ctcaactgct aactttattc tatcaacagc tttttgcata tctttcatta aaaaagggtc
     1801 tcttaaataa tctaaagatg gattcataaa tatttctgaa tctttttcag tacatatccc
     1861 tctatttttt aaaatttggc agatttccgg ggaaatatta agttttttgc taaattcact
     1921 ttcctctatc ttacctttat acttcaatat ccattttttc aaatttaatt ccccctaatc
     1981 ttttttgtat tttgacctaa tacttcttat aactaagcaa aggcctatta taaaggatat
     2041 aaagaaaccc gctattgcag taaattttat ccatatatta ttttctatat ttggagatgc
     2101 aattataaga gaagaaccta ctataataga tgccaacact aaactcaaag atatttgaga
     2161 tgttagctct attatacaat tttctaacct cgtcatttta acatcttcaa tttgcatttt
     2221 tatattattt ctttctatat tttttaaaat tgcttttaat tgctttggaa tagtttttat
     2281 gtccagtaat atttcttcta cattttgtct tgaacttaaa acaacatttt tagggttaaa
     2341 tttactctta taatgatgtt tcataaactc ttttcccata gaaccaaaag aaaaatctgt
     2401 gtttaaagtt ctacttgttc cttctaatgt tataactgtc tttgcaagag ttacaaattg
     2461 tgcaggcatg actattttat actgtctaaa aaacctaaat agttcattta atatatctgt
     2521 tacatttatt ttttctatag aaatatcata gtaataatgt attagataaa gtaaatcctg
     2581 tctaagtccc ttaatatcag cctctccatt taatgcatcc atttctatca atagatatat
     2641 tatcttgtca acattctttt ctacacctgc taatgcaatt tcatttaatt ggtttaaagt
     2701 tttattatca ataattccaa tcattccaaa atctatatat gctatcttat ttttagcaac
     2761 tacgaatata ttacctggat gtgggtctgc atgaaaaaat ccatgagata atacctgtgt
     2821 aaaaaatgac ctaacgccta tttcaacaat agtttttgtg ttataaccta gtctccttat
     2881 tttttctaca tcacttaact ttgtaccatt aactttctcc attactaaaa ttttcttagt
     2941 attatattct gaatatactt taggtatata tacttcatca ctatttttaa atatcctaga
     3001 gaatttagtt gcatttatag cttcaaatgt ataatcaagt tccctcatta attgagtttg
     3061 aaattcttca atcatctgta ctaaatctaa attaaaatct ttttttaaat cttttaaagt
     3121 atttgcaata gttctaagaa tctctaaatc agattttatt attttctcta tatttggtct
     3181 ttgaattttt acgattactt cttctcccgt tttaagtaca ccttcataaa cttgaccaat
     3241 tgaagctgct gctattggtt cttctttaaa ttctaagaat atttcttcta tacttaaacc
     3301 cacttcttct ttaaatatat caatagctat atttgaatca aatttctcta catcatctct
     3361 gagttttgat atttcatcaa ttatatcctg gtctaacaag tcttttcttg tactcaatat
     3421 ctgtcctatt tttatatatg taggtcctag ttcttctaaa actcttttca ttctctcacc
     3481 agttgtcata ttttttattt cttctggtgg gtcaaatagt ggtattttat aggcaattcc
     3541 ttctatattt agtctttcta caataaaact aaaaccatac tttattaaaa catatccaat
     3601 ttctctatat cgctttaaat ttctataact tattctcaaa tcttttcact cctaagatag
     3661 gtatacccaa aaacttacac cttaatttat cgtatatgac attatttcac caattgatat
     3721 tctaatatcc tatatcctct ttaatattta attttatcac atatactacc aattttatta
     3781 aaattattta actcagttaa tttttatttt tgacatttta tatctggttt aatcaaatac
     3841 tttttgaaaa ctaaaatatc aacaaaatct aataaaataa attagtggtt gcctcaaaac
     3901 agatttaaat atgttttgaa acaaccactc tttaaactaa tatttttgat tttgtgctgc
     3961 ctctaaatac cattcataag attcatcaat atatttttta tcttcttctg taagctctct
     4021 aacaacctta ccaggagaac ccattatcaa aacacctggt ggaaacttct tattagatgt
     4081 aattagtgta cctgctccta tcagagtata ttctcctatt tcagcattat ccaatattat
     4141 tgaacccata ccaattaaca cattatcact aattttacaa ccatgaacta tagacctatg
     4201 cccaactgtt acattgttac caattatagt ttctgtatct ccatgcacta tagaacaatc
     4261 ttgtatatta gtgttttctc caatagttat tggtccttca tcacccctaa ctactgcatt
     4321 gtaccatata cttgaatctt tgcctatttt aacatttcca attacatctg ctgattttgc
     4381 cacaaataca ctttcgtcaa ttaaaggttt atcttctaaa taatctctaa tcatatataa
     4441 acaactcctt taaaatataa atttagttgg ttgctccttc taacattaac ttagcttgta
     4501 tcattataga gcactctgta cgttttcttt gcatttcaca gcctaattca tatggttttg
     4561 atatgtcact attaaagttt atcgcatttg catgacatcc acctgaacag taaaacttat
     4621 tccaacattg tttacactct tctttagtat atacatgagc ttctctaaac atatttttta
     4681 aattttctgg aagtactatt tcttcatcaa atatatttgc cattttaaat tcttcatttc
     4741 caacaaattg atgacatgga tagatatctc catttggagt tacagaaaga tactcatttc
     4801 cagctccaca tccagttatt ctctttatta cacatggacc ttgatttagg tctatcataa
     4861 aatggaaaaa tttaaatcca tctccttgta attgtctatc tgcatactct acagcaaatt
     4921 tttcgtactc ttcaaatatc ttaggtaaat cttcttctct aagtgcatat ggattactct
     4981 catctccaac gactggctct acagaggtta atttaaatcc taaatctgca aaatgcataa
     5041 catcttttga aaaatctaaa ttatctcttg taaaagtacc tcttatataa taatacttat
     5101 cttttgctct tttttccaca agtttcttaa atcgtggtaa tgtaatatca taacttcctt
     5161 tatcatttaa agttggtctc atattatcat taacttcttt tcttccatct aaacttaaaa
     5221 ctacattatg catattttca tttatataat ctataatctc atcatttaac agtacaccat
     5281 ttgtagttat tgtaaatctt atatttttat tataatcttt ttctacactt cttccatagt
     5341 caaccagttg ttttacaact tcaaagttca tcaaaggctc tccaccaaag aaatctatct
     5401 ctaggttctt tctacttcct gaattagcaa tcaaatagtc aatggctgcc ttaccaactt
     5461 caaaactcat aagttctttt tctcctccaa aatcaccttg agctgcaaaa cagtatttac
     5521 attttaagtt acaatcatga gctacattta aacataaagc ttttactaca ggctctctat
     5581 gtacaaagct tggatgatat tgatatgtat cttctgtata taaaagaccc tctttttcca
     5641 aatttaatat ttcttcatat gcttcattta ttttttcttc ttgatatttt gattttaata
     5701 tctctaatat ttcttcttta cttttttctt tataaaaatc taacaaatcg tatgcaacat
     5761 catctaacac atgaacagct ccaccattca catctaaaac gatattatat ccattcatag
     5821 aaaatttatg tatcatactc ataccctacc tcctaaatta ctatctatac taaagttttt
     5881 tattatttta attaaacttt acccttcatt atatcataag tttacataaa atacatacaa
     5941 tacaaaaatg acttactctc cgtaagtcat ttcattaaat acactatatc cccctaaatt
     6001 ggcgcactaa tatattacca aataggtatt aggctcctat aaaataaata aaataaattt
     6061 tgtatctgat tttatattgt tttaatctta ttaaaatata ataatttctt ttttcctgat
     6121 aattttgtat aaataagtgc ttgtatttaa tttgtcttct ttagattgct gatttctttg
     6181 ggtactttag gctgatgaat aatccaagga caggtactat tagctgacaa aatagcagtg
     6241 ctaagtgcca atgagctagc aaacttcata aatcttacaa taaacttttt cataacttaa
     6301 ctctccctca tacatatttt acttatactt tagcttttaa cttacctaat actaacatca
     6361 caaaaatcca aaacatggca aatgcgctat atgtgaaata atctacaaat atactcaaag
     6421 aaagacttaa aattgttatc aataacacta ctgtagatat aaatatagca gttttcttat
     6481 aaaccaactt ttccctatca cttatagggt tactcctatg ttctattggg cacagaacac
     6541 aaattccaac ccaactaaca cttgcaataa tcataataat atttttaaat aagtctatat
     6601 ttatattttc tataattaag attgtggata aaattataaa aataaaggtt aataagcatc
     6661 taaaataatt gtctgcatga taccctcctg aaaattgtct tattggacaa taacacatta
     6721 aaaaaaataa tacatatgta aatttaccaa ataaaaaacc tataaacagc atggtggtta
     6781 tattaactat gaaagctatt aatatttcaa atccataaga atagacttta gattcattat
     6841 tatctatcat attattacaa attaaaacag atgtcatttt ttcagcatat cttttaaaca
     6901 tttttacctc caaaaaaatt ttacatatat agagtacatt cttttttgag aaaattcaat
     6961 atactttaca aaactagttg ttaaaaatgt aaaactggtc aaaaaaagac ctatataatt
     7021 ttcttcttat tatttatata acaaaacaat tataataaaa caattttatc aagctttata
     7081 tgcataaaaa gcctatgtca aaatagaaat aaaccttttc cccattgtta aaaagctaaa
     7141 aatatatttc atttcatata aaaagagtac ctcacaaact aaaaagttcc ttttgagata
     7201 cccattttat ttattacaaa atatcaaatt ttatttattt gcatatattc cccatatatt
     7261 ttagcctttt aactttgttt ttgttttaca tactgtagtg cttttctatt ctgttttact
     7321 gcttcaagac aaactttttc tgtttgatta tcaacaaatt ctaaagctct ataattttgc
     7381 tttactgctt ctaaacatat tttttctgtt tgattattta catatttcaa cattctataa
     7441 tcttgtttta ctgcttttag acaaacttct tctgtttgtt ctttcacata tttaagcgct
     7501 tcataacttt gattaactgc ttctaaacaa atcttttctg tttgttcctt tacataagct
     7561 aaagcattat agttttctct tactgcctct aaacatatta attctgtctg attttctaca
     7621 tatttaagaa tttttccatt ttgctttact gcttctacaa atattctttc tgttggtatc
     7681 tttacataac ttaatatttc ttttatttca agttcttcaa atgaacctct tactgcttct
     7741 atacatatat tttcagtctg tttttttaca tattgcagta cttttccatt ttgtctaact
     7801 gactccaagc aaatttcttc tgtttgctct tttacgtact gtaatgctct tccatcaatt
     7861 cttactgctt ccaaacaaat ctcttctgtt tgcttattta catactgtag agccatataa
     7921 cttcttttta ctgcttccat acatattttt tctgtctgtt catttacata atacaatgca
     7981 aagtcattct gttttactgc ttctatacat atttcttcag tttgctcttt tacacaagat
     8041 agtgcactgt agttttcttt tacagcttct aaacatatac tattactttg ttcctttaca
     8101 taagctaaag attttccatc tttttttaca gcttctaaac atatggctgg agtttgctct
     8161 tttattcctt ttaatatctc tgcaacatca tttttagatg ccctttctat aaatgctaga
     8221 catacatctt ctgtttgctc ttccacaaat ttaaatactt ccatgacatc ataagttgat
     8281 gcatttttta tagcctcaat tagtacattt tctgtcttat tatttatgta gtacaaagct
     8341 ctgtagttct gttttactgc ttctatacaa atatcctcat tttgatttgt tatatactta
     8401 agagatttat atgtctgttt tactgcttcc atacatactt tataagtctt gtcctttaca
     8461 aactcaatta tagtaccatc ctgctttact gctagcagac acatctcttc tgtttggttt
     8521 tctatgtctt ttaactctct gtaatcatat tttactctgc ttaaatcatt taaatgtact
     8581 ttgttatctt cctttaaatc ttcttcatta ttttctatat catactcaaa ttcttcatca
     8641 tctaaatagt catttttttc tggtaaaaat atagatttaa ttttattctt tatatccata
     8701 aaattatcct cctttaatat gattctatca ttaaaataaa tcgtttttaa gcacttattt
     8761 aattaatttt atattaagtt tatttaaaag tctattcatt gtcgatagat tatattttat
     8821 attaataatt ttatcttata tatgtggggt aataaagtta atagtggaca ttttccatcc
     8881 cattagtata cactatttat atttattact catttatagt gatttttttc aaattttttc
     8941 ttatttacta tatcatattt attcaattta tgatattatt taaatataat ataagtttta
     9001 taaagtatat taaatatatt ataagatgga ggtcgagtaa ttttgaaagg aaacatagta
     9061 caatataatt ttgcagatat agaggaagaa gtatattcgt tggattatgc tatagcatgg
     9121 aatacagacg aggaaaacgt aaacataata ccatttacaa ataaattctg taaagaatct
     9181 atagaatctt tttgcctagg taaaattaat aactttgttg aaatattaaa tgaaggtttt
     9241 gttgaaaatc atcattatgt acatttagat aaaatgatta gtgttcctaa gaaaaaggtt
     9301 aatttagtat atcaacaaga cacacatggt tatcttctaa gagatgataa tgacaacctt
     9361 attcctgcaa aaataacttc agagcaatct aaatctatat caagtaagat ggagttattc
     9421 tgtgctggtg aagaaaaatg tttaattaat atattactaa aggctgaccc atcatatatt
     9481 ttagatgtag atagtataaa agacaaaaac attctaaatc taggttatga gtctatcgac
     9541 agatataaag aatacaattt tgatgatgat aagattttaa tattttttat aaataaaaaa
     9601 cgttattcag tgattatgaa aaaaacaaac aactctgata acgatttagt ttccagaaac
     9661 aatgctatta aagaattatt tactaataaa gcaggaaatc ttaactagta ttataatttt
     9721 aaattatgtt attgattaaa ttaataatag tatacaaaga ggctgtctca aaatagagat
     9781 aagcttcttt ttcattgttg aaataataaa atatatttaa ttctatataa aagagagtgc
     9841 ctcataaact aaaaagttca ttttgagaca ccctcctatt tataataaac aaataaatcc
     9901 ttaaacctat cgagtgatta taatttttag attgggttat cttgcatttt taaatttaag
     9961 gtaactttct actctaaact ttaatctcta atcaatatca tcaactgtaa caacttacaa
    10021 ttaaatcttt aataatcaaa ctttagacta tctatattaa aactcaaatt agatttagga
    10081 tttatttgga tatcacattt attttattaa ctactaatca attgttttta cttataaact
    10141 tattcttccc taattctatt attaaatact tttaattatt tttcaatacc ttaaatcttt
    10201 attttattaa cctttattct tcaatcataa gttcaataaa tttatcttca cttaaaggtt
    10261 ctgaaaaata atgaccttga atcaaattca cacctgaagt tacaactaaa tccagagttt
    10321 cttttgtttc aacaccttct gctacaatat ccatgccatt aatcttagca atttgaacta
    10381 tagtttttaa taattcaaat tgttttttag aataggtaat ttgttctact aattccttat
    10441 ctatttttaa attttcaata ggtaaatttg ccagataatt tatactacta aatccagaac
    10501 caaactcatc taaagctatg ctaatgcctt ctttttttag tttatcaaga gtaacattta
    10561 caatttcaaa agtttccata agtacttttt ctgtaacttc aacagaaata tactttttat
    10621 ctagaccaca attctcaata catgataata tgtcatttgc acactgctta tgtacaatct
    10681 gctgagcaga taaatttaat tgcatttgtt caaagtctat atttttatct tttatttttg
    10741 tagttacatc acaagccttt tttagtacat atagacctac atccttaata agtccatgct
    10801 tttctgcata agtaataact tcataggcag aataataacc gccctcttta tttctcaatc
    10861 taattagtgc ctcagctttt atataacagt ttttactaac attaaaaatt ggctgatacc
    10921 aaacttctag tgtttgttct tccagacctt tttttaaatc cttaataata ttattttcat
    10981 ggtaaatctc ttctgtcaat tccttatcat aaactttaac attacttttg acatcctttt
    11041 ttgcatactc caatgcaatt tgtacctttt ccagaaggtc atatggattt tcaccatgat
    11101 atggatattg acaaatacca atattacatt ttacatcaaa aagtacatct tctacactta
    11161 aagaactatt aaatacttca ttaaccttat gtaaagtatc taagtaagat tctttatcta
    11221 ttgaaatgcc taaaaataca tctccattaa tacgatatag ataattagaa aaaattgttt
    11281 ttaatttgtt actaacttct tttaaaataa catctcctat ttcaatagaa aacagttcat
    11341 taaattttcc aaacttgcat atgtctatta aaaataagta taacattctc tcagaattat
    11401 aatcaattaa tacttcgaca tcacgcatat accctcgcct atttccaata tcaatattat
    11461 aatctttata cataatacgc tgtacttttt tataatattt gtatattact ttagacaact
    11521 tgtttatttc taaatctgga tattcaccaa atacaatctg tttcttttca tcaagcatat
    11581 cttcacactt tttagtaaaa tcatgaatag gagacattgc tggaaatctt ctctcataat
    11641 cttcaatagc tgaaagtaac agagattctg catcttttct atcatcaaca cacctagatg
    11701 agccataacc aatcgttaaa aatacttgtt tattttctac tttaattttt cgtaaaagct
    11761 ctaaggaaat ccctttttca atgttaagca attcattttt atcacaattt tcttctaaag
    11821 ctagaacaaa accttcctca cttatacgag caataaaatg agaacttttt atgtaattat
    11881 ctagattttt tactatatgt gtaagataat cttgtctttt ttcaaagctc ataactggag
    11941 caaatttact ataattagta atacatatat acagcaacac tatttcatta ttttttctat
    12001 tcagatgctc ttgtaactta attaaaaaac cttctctatt caaaatacca gtttctaaat
    12061 ttttaagact ctgttgattt aattccttac taatcgactt cttttcaatg taaataaatg
    12121 tcatcacacc tattaatatg gaaataatta acaatataac aaatacaata aaatctatat
    12181 tcttattatt ccatccattt ttgtacacaa tgctaagagt ccaaacagtc ggaagtttaa
    12241 acttaatttc atgtgatttg gaaaaattaa aatctttagt agaaatttct attgtagatt
    12301 ttgaaccatt atctaggtgt acacgccaaa gttcatactc aaatccagat tcttttaatt
    12361 tatttaagtt aaaagaatct acaacttttt tggcatctaa agctactgta atttctcccc
    12421 agtactctcc atttttataa ataggattag taattaaaaa cacatctcca tttgaaaact
    12481 ggtctttaga aggaccttct acgactaatc ctttgataga atttgctaat gaataagaat
    12541 aattcagttc ctgtattgga gtattgataa tatctttttt gacagaatta gaaaaaaatg
    12601 cagctacatt atatttttct acaactgaaa tatatatgca agaattgttt ttaagaattt
    12661 catctgcaat aggttttaat ttatctatag aataattgtt tgaaactaaa actgatgtca
    12721 agtctttgct ataattaaat ttttcagt
//
LOCUS       DAFKMX010000119        12390 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFKMX010000119 DAFKMX010000000
VERSION     DAFKMX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.119"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            476..2710
                     /locus_tag="LZB08_002585"
     CDS             476..2710
                     /locus_tag="LZB08_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7316077.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSKKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2741..3586
                     /locus_tag="LZB08_002586"
     CDS             2741..3586
                     /locus_tag="LZB08_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7316078.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3826..5559
                     /locus_tag="LZB08_002587"
     CDS             3826..5559
                     /locus_tag="LZB08_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7316079.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5951..6377
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6016..6044
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6657..6809)
                     /locus_tag="LZB08_002588"
     CDS             complement(6657..6809)
                     /locus_tag="LZB08_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316080.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            7934..9289
                     /locus_tag="LZB08_002589"
     CDS             7934..9289
                     /locus_tag="LZB08_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7316081.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(9407..10075)
                     /locus_tag="LZB08_002590"
     CDS             complement(9407..10075)
                     /locus_tag="LZB08_002590"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBM7316082.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(10142..10570)
                     /locus_tag="LZB08_002591"
     CDS             complement(10142..10570)
                     /locus_tag="LZB08_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBM7316083.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            10677..11270
                     /locus_tag="LZB08_002592"
     CDS             10677..11270
                     /locus_tag="LZB08_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBM7316084.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11390..12325
                     /locus_tag="LZB08_002593"
     CDS             11390..12325
                     /locus_tag="LZB08_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBM7316085.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPNDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
BASE COUNT         4691 a         1599 c         1657 g         4443 t
ORIGIN      
        1 tgaatattta ttatagtctt taacaaaaaa tacaagaata aacttaattt taataactaa
       61 aattaattat gcaaaattat taatatggta ttaaattatg tataaaaagt taattatgaa
      121 taaaataatt atgcctttga aaaatcatta aacttaaaat taattataaa aaaactctac
      181 cttactaact atacagctca caatctatct atttatgaaa aaatatgata taattgcatt
      241 caaaaaggaa atctttcaat atactagaat ttaagcatta actaatttta tctaatatta
      301 ttactaaact aaaaaatatt tgtatttgta gcattggagc aagtaactta atgtatattc
      361 attttaaata cttatttaaa atttagcatt taaattattt acaaattaac atttaaatac
      421 ttctagcata aatacagttg tcatcaaaat ttaaagtatc ttatggagga ataccatgca
      481 aaatcataat tatataaaaa tgttaaataa aaaggtagaa gtattatcta aggaattaga
      541 tagtagaaat tttaaattaa aaaccttaaa tgaaatagat tttttttcta aaataccaaa
      601 cagagaattt atgtaccaac atattgatga ctgtatttcg aaaaatatac atataggaat
      661 catgttatta aatatcgata actttaaatt atttaatgat atatatggat atctagtcgg
      721 agaaaattta ttaattactc taactcaaaa cctattgcac tttattccag aatcaaaggg
      781 aatagtcgct tatttaaaag gagaaggttt tttaattgta cttccaaatg tatcaaataa
      841 agagttaaat aatatgggtg caactattat agagagcgcc cgagaattaa aaataaaact
      901 tcctaatgaa aaagaaatat actccaatgt tacatttagt atcggcagca ctatctggaa
      961 tggtgaacca aacatgacta ctttgatgct tttcaaccaa gtatatagtg ctttaaaaaa
     1021 ggctaaaagt gaaggcaaaa atagatacat tctatttgat tttaaagata atagttttta
     1081 caataaatta gaaaatgata aggagttctt atcagaaata tcacatgttc tacaaactaa
     1141 acaaattgaa gtattttacc aaccacttta taatataaaa aataatacta tagttggatg
     1201 tgaggcactt gcaagatgga gacaccctat taaaggactt cttactccag ataaatttat
     1261 tcctttattt gaacagtttg gtctgataac ggaattagat ttgcatttat ttgaagagaa
     1321 ctgtaaaaat ataagaagat ggattgataa caatgatgtt tttgttccaa tttgttgtaa
     1381 cttttctcgt cttcattttt ttaaccctaa ttttccaagt atgctaaaag gtattactga
     1441 aaagtacagc gtctctacat caaattttag cattgaaata acagaaaata tattaataga
     1501 aaatacaaat acaattattc aacaactagc tgaacttcgt tcttatggtt tttctgtatc
     1561 aatggacgat tttggttctg gatattcatc ttttggtatg ttgcaaaatc taccttttga
     1621 tataatcaaa attgatagga tatttttatc tcataattta aatgatttta aaaatacaac
     1681 cattttgtac tctataataa acattgcaag agctcttggg atgcttacag tatgtgaagg
     1741 tgttgaaaca gaacaacatg tagaatttat taaaagtata aattgcgata tcgcccaagg
     1801 ttattattat gcaaaaccca tggagtgcaa agaatttgaa aaattactat ctaaagagaa
     1861 taattcaaag aaaatgttta taaaatattc tgatatagga aaatctttaa tagagaagat
     1921 atttgatgga ttttttatta tgcaagatat gaaaaaacta agagctaatg tcactgaaga
     1981 cattcaatgg tttgattttt acaacaaaac tgaaattaca tcttttccta aagtaataga
     2041 acattttgaa aaacatatta aaggtaaaaa attttctatt ctttacaaaa gtataacccc
     2101 acataatact gaatcagatt cattattaat tagtggagaa gccgttttat cagatgaagc
     2161 ttctgaacat caaaaatata gcaatttcta ctttagtgca aattgtataa tttcagaaaa
     2221 taaattactc ttagcaaaat tacatttgga tttaattaaa actgctggct atataaatga
     2281 atttgaatca tattcaatac ctaaaatgac aaatacacat cttgatgaaa gtaatatttt
     2341 atcacaattt tattcaatgt tgcctgtagg tataattcgc tacgatttat ttggtgatat
     2401 gattattact tatatgaatg aagaaatgtt tgatattata ggttatacaa aagaacagtt
     2461 ttttggtgag atgggcggaa atttaagagc aattgttcat ccagatgatt tagactttgt
     2521 ttataaaaaa agtttggaca tgattgacac aaataaaatt gaaccatttt tatatagatt
     2581 cataaaacga gatggtcaaa ttgtcacagt tatgtataca caatgtaacc tttctgcaat
     2641 tgatggaagg tcgattgccc agggtatgta tgtagatgta aataaaataa aaaatttaac
     2701 aaaaatctag tacaaagtat tttattttgg agaaattaat atgtttaaag aaattttttt
     2761 aagaactttt ccaggatttc taattataag agattctaac tacagaatta tatttataaa
     2821 tgataattta aaaaatttaa ttaaaagtta tatgcaagat aatccacttg gaatgactaa
     2881 tatagaaata gctaagaaac tccctgataa tattgctaaa ttttttacgg attctcacaa
     2941 tattcaatta gattgggaaa aaaattatcc ttatgataaa atttcaaatt ggattttaga
     3001 gtttaaaaaa gatactacaa gctattggaa tgttttagaa tataaagtag atgttgatga
     3061 aaaaacctac ataattacta tggccaatga tataacaaaa ctctatgaag aaaataagag
     3121 aaatcttcac tactcaatta ctgacccatt aactggagca tataatagaa aatatttaaa
     3181 tgatagattt gacatattta taggagatta tattgtacta attgacttag ataatttcaa
     3241 aatgataaat gactatgaag gtcataatgt aggagacaag attttatgtg attttgtttc
     3301 acttttaaat aaagaattaa taaattctac ttctattata agattaggag gagatgaatt
     3361 tattgttata ttctctagtg atgttgacaa aaattatgtt tattcacagc tagaagcctt
     3421 aagagaaaat tttttaaaag tattctctaa gtataagtat ctttcattta gttatggtgt
     3481 tgatacagta aaaagaaatt taaaattgac aatagttgaa ttggataaaa aaatgtataa
     3541 aaacaaggag aaaaacaaga aaaaatttga taaaaatgat tattaatatt gttattttaa
     3601 aataaattaa aattatcttt ctttttttaa acttgtacat tagtttataa atatgtatct
     3661 atacaagata ttgacattaa gtactgaagc atatattgtt tcagttcttt tttgttaaaa
     3721 ttaacttatt tgacaaagat atcatagttt aacaaagctt ttaatttaaa tatattgatg
     3781 tataatataa ttaaaatata atagcaaaaa gaaaggagga atatcatgag catactccta
     3841 aaaaaagcac ccaaattagc aaaacatatt ataacaagtt tttatataaa tcgggatatt
     3901 gatgaagtat taaaatacct ttgtgaaaat gttacatgga ttggaccagg agaacaggaa
     3961 tttctcactt cttttaatga aataaaaaat tatttttatg caggtcaaga tgaaattcct
     4021 tcttgtgata taaataatga tatatttgaa actgtaagtg aagatgagaa tagatgtatg
     4081 gtattaggaa gatatactgt tcgaactaaa gaaaatgctc aaatgatttt agaggtaaat
     4141 caacgttgta catttgagat aattgaggat agaggaaaat tattaataaa gcacatgcac
     4201 atatcaaacc cttatggtga aatgcaactt gatgaatact ttcccactaa aattggaact
     4261 caaagttatg attatttgca aagattacta aaagaaaaaa cagaagttat tgaaatgatt
     4321 acaaataata tcaatggtgg cttaaaaggt agcaatgatg atagtacata ctcatttttc
     4381 tatgtcaatg agggattgcc caaaatatta ggatacacat atgatgagtt catggaaatg
     4441 agtggtggtt cagcagtggg tgctgtatat ccaccagact tacctaaagc actagaagat
     4501 tgtgaacgat gttttgcaaa aggacctaca tattcatctg aatatagaat ccgaaaaaaa
     4561 gatggaacat taatgtgggt attagattct ggaatgaaat cgttaaatag tgatggcata
     4621 gtaaaaatta atagtattat tactgacata acccaactaa aaaacataga atctgaacta
     4681 aaattagaaa gagagcgata tcgtattgct ttgcaaaata ttacagatat catgtttgag
     4741 tatgatattg aaaacgatac ccttataaaa tatcaacgtg tagaaattga caaaaaaatt
     4801 gaattagaaa attttgaaac aaaaaattac tccaaactat tagaatctgg taaaattatt
     4861 caccttgatg atattggaaa actactagaa gtacttcatg gaaatcttcg tgaaacaatt
     4921 gagataagac aagtaaattc attaactaaa aatgaatgga gatggattcg tgttcaatgt
     4981 tcagttatat atgatagtga ccataaccct atcaaaacaa taggcgtttt aaaagacata
     5041 actgaagata aatctaaatt agaaagctta attaatcagg ctcaacgtga cccactaaca
     5101 caattatata atcaaagagt atcacaaaat cttattgaag agtatctatg tagttctgat
     5161 tccaaaaata atgatgctct attagttatt gatattgatg attttaaaac tgtaaatgat
     5221 acttttgggc atttagaggg aaatgaagtt ttagttgctg tatcaaaaat actattacat
     5281 aacacacatg ataaagacat agttgctcga attggtggag atgaatttac aatatttata
     5341 aaatcattaa caaaggattt aataataaaa atcactaatg atattttaaa tgatgcaagt
     5401 aaaataaaag taaaggacaa tcataaaata acattaagta ttggtattgc cttcactgat
     5461 gattcaacta aattatataa agatttattt tctaaagctg ataaagctct ttatctatca
     5521 aaagcagatg gcaaaaattg ttatagtaca tacgaataat aaaactattt atacgaccaa
     5581 gaatgaacct ttaaatttaa tttcatagtc taacaatcaa agaattgaag ctaatcttat
     5641 agctagtcaa ctagttccta gaaaaaattt agacaatata tttattgata ctgctcattt
     5701 ttctaaagag caaatttata taaaaaatta aacttttatg tacatacaga tactaatgat
     5761 aaacagttat cattagtatc ttctatctta aaacaaaatg ataatttatc tatataaaaa
     5821 agtgctttgt tgacagagtt cactttttta tataggtaca gaatttaagt ataaatacat
     5881 gttttattct atacatttta gttaatgtaa aatttcttga ttgtactctc taacttttca
     5941 acattatact atttatatac cacttggtta atataaaact attattatag tgtcgtgtgg
     6001 agggcggtgt gacttattta cataccactt agttaatata aaactctccg ccccattttt
     6061 catcatatta aacatttcct catttacata ccacttagtt aatataaaac gaggtgcttt
     6121 caaaattgac gaagactgta gaaaaatatt tacataccac ttagttaata taaaacttat
     6181 aagttgtttt atcagcaaaa acgtttatat tactatttac ataccactta gttaatataa
     6241 aacgataaaa gcatgaataa aattgaagta gctaaaattt tatttacata ccacttagtt
     6301 aatataaaac ccagcttcaa tttctttagc tcaaataata ctagaaagat ttacatacca
     6361 cttagttaat ataaaacccc aaaataaact gggcattttc aatgcttaca catgtgcaac
     6421 tctcttaaat ttgcagtgaa ccatgagtag tgcaaatgat aaagcttatc acatgccctc
     6481 aacaccttat atttcaagca ttaagcccta ctttgtaaca aatatcgctc actgcaaaac
     6541 tatttacatt tttattatat cataaatata ttatttttga atatctgtac caatttgtgg
     6601 tataataaaa acaagaagaa ctacaatcta tttagacgta gagtgaagtt caaatactaa
     6661 tgtttattct ttttgaactt gatttttagt tcaaaactaa agtcgctcct gccagagtgg
     6721 ctttttactt ttttaaataa tttactattt aagtaaacta ttaaactggc aactaaactt
     6781 gctagtacac cttgtaaaaa attatccata catactcacc tcccttctat acattgggag
     6841 gataatcttt tgtatgaact ccactctata aattgtagat tacatcttct tgctaaaaat
     6901 gttatagcat ataattctta catattttac ctatatatta cttttctaaa gattcgtcta
     6961 tatatttctg tatacatcta ctctatcctc gatttgttac aaccccaatc aaaaaagtag
     7021 ttcaacttct aagttttata caaagcagga aactcaatta ttattagaaa aatcaaaaaa
     7081 tactcctata ggtataccaa tttttttgat gctaactctt ggattacgtt ttggtgaagc
     7141 tgtatcactt atatggtcag atgtagacct tgataaaaaa tctcacatgt aaatcaaaca
     7201 ttagtttatg tatacaataa aataatcttc aaaggcccta aaacccctag attcgaaaga
     7261 agattatttg ttcctgatga acttataagt ttgcttaaat gggaaaaatt aaaacaaaat
     7321 aagttaaaat tacagggtat attgaaaaat gaatttgatt tgatatgctt aaataaaaga
     7381 ttcaacccgt gagttcaacc aacttttgtt gttcatttta aaaaactatt aagagataat
     7441 aatcctagag atataaaagt acatgaatta atacatgcaa atgcgacttt gatattactt
     7501 tcaggtacta acataaaagc tatttataaa atattagtac atgcagatat aaaaatagct
     7561 atgaataaat attccatatt ctcacttatt agatgaaatg aataaagaaa tatctgaaaa
     7621 tataagcaaa atactattca aataatgttc agttttatgt caattaaatg tcactcaaag
     7681 tgcttttttt ccatttgtca gttaaatgtc acttaaagta cctttttcct atttgtcagt
     7741 taaatgtcag taatatataa ataagatata tttataagac ccttaaaatc actattattt
     7801 aataaaattt ataattatat actatttgct gttgacattg gtattatacc atagtttatc
     7861 atttatatta gaaataaaat tattaattaa tagtttcgta attttataat agatttacag
     7921 ggagtgataa taaatggata caaaaatatc tataaatgaa aacatgagtc taggtaaaag
     7981 attttttaaa tacctagcac catctgtagt tgcaatgtgg gttttctcac tatatacaat
     8041 ggttgatgga atatttgtaa gtaaaggagt tggagaatta gctttagctg ctgtcaatat
     8101 atcaatgcct tttattaatt ttatatttgc agtttctcta ttattttcaa ctggtgcatc
     8161 taccataatt gcaatctatc tggggaaaaa agatattaaa tctgctaacg aagtcttttc
     8221 atttaactta gtatcaataa ttatattatc aataattata ttagctataa ctttttttaa
     8281 tttagataga ttagcactat ttttaggagc tacagaatct acaattggta tggttaaaga
     8341 ttatttagga ataataatat tttttaacgg attctttata gtgtcttatt ctttagaagt
     8401 tataataaaa actgatggct ttccaatatt agctactgtt ggtgtaataa tatctgctct
     8461 tacaaacatc atacttgatt acttgtttgt aattgaattt ggatggggtg taaaaggtgc
     8521 tggtattgca actggtttgt ctcaagtctt ttctactatt ttcttcttaa tacatttttt
     8581 aagaaaaaac tctactttga atttttcaaa atttagaatt gattttaaga cacttagaaa
     8641 aattgtattt ataggttttc ctgattcaac tactgaatta agttgtggta tagttgtttt
     8701 actttttaac ttaagtctta ctaaatatat tggagaaaat gctcttattt attatagtgt
     8761 cataaattat ataaatacat tagtacttat gactatgatg ggtataactc aaggtatgca
     8821 accacttact agcttttatt atggtgcagg aaatatagat aatgtaaaaa aattattaaa
     8881 aatggggata aaagccacaa taatagcatc agttgctgta tttgcaattt gtatggcttt
     8941 ttcaggacca atagtctctt tatttataca tccagaagaa acaatgctat tcaatgaagg
     9001 agttcgtgta tttaaaatat tctctatatc tttcttattg gtaggaatta atgtaataat
     9061 ttctggattc tttgtatcag tagaaaaacc atcaatttct actgttatat ctcttggtag
     9121 agggcttgta atagttgttt taagtctaat ttcaatgata ctaatatttg gtggacaagg
     9181 aatatggatg actactattg tttctgagtt tatatgtctt attttatcat tagtttttct
     9241 aaaaaagaat ttttctacat tagactctaa tctaaataag gttgcttaga tgataagagt
     9301 gacaatatat gaaaatatct aaataaaaaa tacctattat acaatcttgc aaatattaaa
     9361 cctttgcaaa aattaggatt atataatagg tattttattt atgtaattat tttatttcta
     9421 aatatccata gccatctttt attaaagttc cttttacttc atattctaca ggttttttaa
     9481 atattggacc atctacgaca aatgtatgat actctccatg ttctccacat gggtctgcac
     9541 cagcattttt tatcttctcc acaagttctc tagttaattt ttttcctaaa aattcctctc
     9601 ctaagtgctt taagtttact tttgttacaa cactacaaaa tccactgtct ataaattcat
     9661 atactaaatc ctctctactc tcttgccata atggtaaatc tactttcata ccaactgctt
     9721 cacatctatc agtatcccat tttctatgac tttctatatc tatatctcca tatgcacata
     9781 tcgttgctcc catgttttta gcttttatta aagccctctc aaagctctct tcatatcctt
     9841 ctacgccaca ctcagcaact aataaaggta atcctatgtt gctacttact tgcttaagca
     9901 tattatattc taaattatgt gtccatgact ttccatgttc ttcacttata gttgtcaaga
     9961 gtgccacagg cttatatcct tttttcaaca ttcgatttaa tgctaatata ctatccttac
    10021 caccactaaa tgacattaca aatttttcat cagccaattt taacacctct ttcatctaac
    10081 tttgttttat taacataatt ataccaaatt tttgagttgc atagcaaatt atattaaatt
    10141 gttaattcac tataaagaat gtccctaata ttaaaagtat aacccctatg tatttgtata
    10201 aatgcattgt ttcttgtccc aaaattacgc tatcaataaa aaatccaact aaaacttggg
    10261 atacaagagt taatataagt gtatttgata ctcctaaatt tggaattgcc tttactgtaa
    10321 aatatattat tagacctcca aatactcctc ctaataacaa cttataatta aaaccattta
    10381 tagatactag acactttaag tcacctgtaa ctattatact tgccaaaaaa aatattgctc
    10441 ctacagttaa gcttataaag gtggccacta atgctgttgt actttttcct agctctccat
    10501 ttataaaagc ttcaagagct gtagaaccac ctgctaataa tgcaaatatt attgcccata
    10561 attttgtcat ggacaaacct ccttctatat aattctatta atagaagttt atgatacaac
    10621 ctattaatta attactaagt atagtttaat ttttatacac taggctttat ttggatatga
    10681 atttctgtta taattgttct atcgattttg gaggtgattt tatgtacaca aattttaata
    10741 tcgattttaa taactttaat aaaaaagaaa atgctattaa atttatgtta atgggagtat
    10801 tactcattat attaggatta ttatgcctta catttaagac attaggtata aaattaattt
    10861 cttggacttt tggtattgca cttttattct ttgcatattt aaatttaaaa aatattaatg
    10921 aattaaaaag atatgctact aaagaagaaa ttaaaccctc tattaatatc caatgggttc
    10981 taataattgc atgtatatta ctttttgtat ttccgcaaaa aatacaaagc atattttcat
    11041 tatttttagg attttattta atctttaatc aactagtagc acttgtaaat agtaagaata
    11101 atccttattc taaatttaca acttggaata tagttaagat tctatttggt atatgcctta
    11161 tattatctcc acttttttta tctagattta tagtatcgat aatgtctttt ttcataatat
    11221 tatttggact agttctattt ttctctggaa atacagcgag aaaatattaa ttattttctc
    11281 gctgtatttt tatattactt aaaatagaat ttgtttaagt tatctatttg tggtaatatg
    11341 ttttttgtac aagctcagcg tacaatttta ttagaaatag gtgatttttt tggataaatt
    11401 taaatatgct tttgataata aaagatatca tacttggaat tactatctta gaaatacttt
    11461 tggagaaaaa gtttttaagg tatctataaa tgcaggattt acttgcccaa acattgatgg
    11521 aagtcttggt tatggcggat gtacctattg tagcaaagaa ggctcaggtg attttgctgg
    11581 aaaccctaaa gataatctta tcagtcaatt ttataatata aaagaaatga tgttaaaaaa
    11641 atggcctcat gcaaagtata ttggctactt tcaagcctat acaaatacgt atgctcctct
    11701 tgaagtttta aaagaaaaat atgagactat attagaactt gaagatgtca taggtctttc
    11761 aatatccaca agacctgact gtttgccaaa tgatgttgta gaatacttgt ctgaactaaa
    11821 taaaagaacc aatttatggg ttgaacttgg acttcaaaca attcatgatt ctacatctaa
    11881 aattataaat agaggtcatg attataaaac atttgttgaa ggtgttgaaa aattaaagtc
    11941 taaaaatata aaggttgtcg tacacattat aaatggtctt cctggtgaag attataatat
    12001 gatgatggaa acagcaaagg cagttggtaa aatgggtgta gacggtataa aaatccatct
    12061 attgcatgtt ataaaagata ctcctatgga aaagatgtta caaaatggta tgttaactct
    12121 tatggaacaa gatgaatata tcaatcttgt ctgtgaccaa ttagaaatac ttcctgaaac
    12181 tatgataatc catagactca ctggtgatgg taagagggat gaactagtag gtcctatatg
    12241 gagcctaaaa aaatgggaaa ttttaaatca aattgatgat actttaaagg ctagaaattc
    12301 ttatcagggc tgtaagtttg tttaatacaa aataataact atagaaatgg ttatataaat
    12361 atagtccttt ctatagttat ttagtattta
//
LOCUS       DAFKMX010000120        11985 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFKMX010000120 DAFKMX010000000
VERSION     DAFKMX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.120"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            123..1004
                     /locus_tag="LZB08_002594"
     CDS             123..1004
                     /locus_tag="LZB08_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBM7316086.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1065..2273)
                     /locus_tag="LZB08_002595"
     CDS             complement(1065..2273)
                     /locus_tag="LZB08_002595"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBM7316087.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     RIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2346..2598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2750..3739)
                     /locus_tag="LZB08_002596"
     CDS             complement(2750..3739)
                     /locus_tag="LZB08_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBM7316088.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3891..4331
                     /locus_tag="LZB08_002597"
     CDS             3891..4331
                     /locus_tag="LZB08_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316089.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGVICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4658..4885
                     /locus_tag="LZB08_002598"
     CDS             4658..4885
                     /locus_tag="LZB08_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBM7316090.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4911..7031
                     /gene="feoB"
                     /locus_tag="LZB08_002599"
     CDS             4911..7031
                     /gene="feoB"
                     /locus_tag="LZB08_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBM7316091.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7164..7982)
                     /locus_tag="LZB08_002600"
     CDS             complement(7164..7982)
                     /locus_tag="LZB08_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7316092.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8189..8473
                     /locus_tag="LZB08_002601"
     CDS             8189..8473
                     /locus_tag="LZB08_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316093.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   8641..9132
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8708..8736
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(9791..11884)
                     /locus_tag="LZB08_002602"
     CDS             complement(9791..11884)
                     /locus_tag="LZB08_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7316094.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         4237 a         1748 c         1451 g         4549 t
ORIGIN      
        1 gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac tttttctact
       61 ttcttcgata taattataat atatttttga tacaattcga caaaaggggg aatgctgtaa
      121 ttatggtaaa aaaaagaaaa aagaaaactg ttacacttac tatacttgtt gtaatagtta
      181 ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc
      241 aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac
      301 ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca aaaatagttt
      361 gggacaaact taacaaatac gactattcaa acaatggaga aaagatagtc tttcttacat
      421 ttgatgatgg accatctact actaatactc cacaagttct agatatcttg aaaagacatg
      481 gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga gctaatgaaa
      541 ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat actcatgact
      601 ataaaaaact atatcctggc agaagcctaa atctcgatac attcgtaaat gaacttaaca
      661 aaactgatga agctatgaaa aaagtcttag gaaagaattt ttcctcaaat gtagttagat
      721 gcccaggcgg gtatatgtca tggaaaaata tggaacctct aggaaactac ttaaaagaga
      781 aaaacatggc atcaattgat tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa
      841 atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa atggttgttc
      901 ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac caaattataa
      961 catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa agcaagttta
     1021 ttgaatatac attttatact caatagactt gcttttactt tactctattt tactagctcg
     1081 atttttacaa attttttctt tcctatttga acaactaatt cactctcttt tacaatagtg
     1141 cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc taatcttcta
     1201 acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa gtcaaatcca
     1261 tcttccttaa cttgaattgt ttgtatatct tcaggtattt gacctttttg gaatacagat
     1321 ttaaatcttt cttcagcttc tttagcagat tcttctccat ggtataatgt tactatttct
     1381 cttgctaagt tcatctttat atctcttgga tttactgaac cctctttcaa ctgagattct
     1441 attctattaa cttcatcagg atgaacatct gtcactaaat tgtagtattt aattataagt
     1501 tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc tattccaata
     1561 tagttaccta aactcttact cattttttct ttaccatcta atccttctat caaaggcatc
     1621 attattacta tttgagactc cataccaaat tctctttgta atgatcttcc cattagtaaa
     1681 ttgaatcttt ggtcagttcc accaagttct atatcagctt ctaatgctat agagtcatat
     1741 gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc ttcatatctc
     1801 tttttaaaat cttctctttc taacattctc gcaacagtaa tagttgctgc taattttatt
     1861 acatcttcaa aatttaactt ggctaaccac tcgctattaa atcttacaat agttttttct
     1921 ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag aacttgctct
     1981 gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt tcctgtaaaa
     2041 tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat ctttctaaga
     2101 actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa ttttactatc
     2161 ataggctttc cttctttttc tgatttaata agcttttccc ttaactcttt ctcatctatt
     2221 aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt catttttcat
     2281 tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa aagctttcat
     2341 cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc aatatctcac
     2401 gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg tactaatccg
     2461 atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt caaactaaat
     2521 caatttattc catttcacca aactggaact ctctgttaaa taaaatatag ccctacttct
     2581 tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat ttttcttaac
     2641 ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat tatatacatg
     2701 tttttttaca ttatactact tttttatatt ttttaccaat acccaaagtt tatttttcaa
     2761 tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata tttattttct
     2821 tatttaaaaa tgcgtttata attattaaac caataactgt tacatcaaat ataaaaaaca
     2881 gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta aacttagcta
     2941 ttataaagtt ttgtagtgac atacatgagt ctctaatgat aaaatagaca cttatatagg
     3001 ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc aacagtttta
     3061 gaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt gagcaacata
     3121 aaaattggtt taaacttcta aaattcttaa atattaaaaa tttttttaaa gaattagaat
     3181 ctaaaatatg caatttattc cctaaaatta aacttaataa atatatacaa aacaccatac
     3241 caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca ttaaaataaa
     3301 tccatttgct atatccttga aattctttca taaaactata acctattaat gttgaagcat
     3361 atatatcaat gaaaaccatt acaataactc caaatactga accatatact aaatcatcca
     3421 taaccattgt aggcttattt attagatagc cacacattat accaagtaat acactctgac
     3481 ttgcccaaat agcagttcct agatttccaa gtaccagaga aattattaaa agtgaagtaa
     3541 ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa aaaaatactg
     3601 gcactataaa atctaagtaa atagcatatc caaatccagt actcacagct ataattgtta
     3661 ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta gtattcaaaa
     3721 cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata tagtatatca
     3781 ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg ttaaaaatgg
     3841 tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact atgtttaaaa
     3901 ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa tcaagatatg
     3961 aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat atagataaaa
     4021 gctcttatca catattttta aaagaaaatg gagtgatttg tgcttatgct agaataattc
     4081 ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt ctttctagtc
     4141 atagaagaaa aggattagca aaacaaatga tggattgtgc tatagatttt ataaaagtta
     4201 acttacacga aaataatata accttatcag cacaaactta tataaagaac ctttacttat
     4261 cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa catattaaaa
     4321 tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat aaattaaata
     4381 ttgaattgat atttaggtat ttgaaaatac cttacataga gtcagaaggc ttttgctttc
     4441 tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa tttttattct
     4501 atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag gcatcttgcc
     4561 tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttaa aataagaaga
     4621 ttagcccagg ctaaatttca agcatggagg tgctattatg aaaaacctta acgatattca
     4681 agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa gagaaagaat
     4741 gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag gtccaaaaaa
     4801 taatttaact gtctataaaa tcagaggtag caagatagct ctacgacaag aagaaagtag
     4861 cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat atggggttga
     4921 cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac aataaggaag
     4981 accagtttgt aattgcattg gctggcaatc caaatacagg taaaagtact gtatttaatc
     5041 atcttacagg gcttagacag catactggaa attggccagg aaaaactgta gctacagctc
     5101 gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca ggaacatatt
     5161 ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt tttggtaatc
     5221 cagatgcagt aatagtagta tgtgatgcta catgtcttga aagaaattta aatttagtat
     5281 ttcaagtgat ggaacttaca gataaagtta ttttgtgtat taatttaata gatgaagcta
     5341 gaaaaaaagg cattactatt gataaaaaac ttttagagga ttctcttggt attccagtta
     5401 ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact ttaaatgatg
     5461 tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa aatatagaga
     5521 atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata aattctaggt
     5581 ggttgggact tagacttatt gatggcgatg aaagtatatt tgaatctatg agtaattata
     5641 tagacaaaga ttctatagat gctattaatg aagtaaagaa aaagattcca gacaatataa
     5701 ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct aaaaaactga
     5761 gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa aaagtagata
     5821 aaatattaac atctaaaata tttgggcttc caataatgct cttattatta ggtactatat
     5881 tgtggattac tatagaagga gctaactatc catccacttt attatctaat ttattactcg
     5941 gatttgagcc aagtatttcg ggtatattaa atagtattaa ttgtccatca tggttaaatg
     6001 atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta atgctacctc
     6061 ctatggcaat attttttcca ttatttaccc tacttgagga ttttggatat ttgccaagag
     6121 ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga
     6181 ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg ataattgact
     6241 ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt aatggtcgtt
     6301 ttcctacttt gatagctata tctacaatat tttttagctc agttataacc aactctttcg
     6361 tatcaagcgt agcaactgca ctttgcataa ctttactgat aattctaggt gttataataa
     6421 cattgttggt ttcttatact ctttctaaga cgctattaaa aggagttccg tctacattta
     6481 cattagaact tcctccttat agagttcctc aaataggaag aactttatat acctctataa
     6541 tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct gctggtgtca
     6601 taacttggat atttgcaaat atatatatag gagatttaag tatattatca catgttgcaa
     6661 actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta cttgctttta
     6721 ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct tacctagcaa
     6781 ctggttctat gatagaatta gatagcttta gtgctttagg tcaagttctt agagaacatg
     6841 gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat tggccttgtg
     6901 caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact gccttaggat
     6961 tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca gtttacaatt
     7021 tatttatata attagtatta actgacagta gacatatatt agaaaaacta tttaaaaatt
     7081 tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt acttaacatt
     7141 tgaatatatc tctattttaa ttcttatctg gatacttgga ttgaaatttc agtcatataa
     7201 ttatcttcat tactttcaca aaagaagtca agtatactct cctcatatga atttccaata
     7261 attttatatt cattttcata tatatatttt ttcaattttt cgtaagattt atatgaatcc
     7321 tcatattttc caatatgttt tatgcaagca tacattcctt ctggctttac atatagcttt
     7381 ttatccttta tttttttatc tatcttcaaa taaaaacttg atatacttaa atagtcccca
     7441 ttttttatat tttcattact tactattact cccaactcat atccttgata caaattttta
     7501 gatttacaat aattggtgaa atctattaat tccatcataa aatcactatt actcatatta
     7561 ctcaaatcgc ttgatagaac taaatattct tcaaactttt tggatatata aatttcttca
     7621 ctattcattt tagaaattcc ttcaactgtt atatttattc tattttgcat cttagaactt
     7681 atctttctta gtttttttat tttttctctt atcttttttt cttcttcttt aagcattttc
     7741 acacaaagct ggggagttct attttgtata tactctttta tttcctctaa actcattcct
     7801 atttctctca atgattgtaa tatatcaagt gtatcatatt gataaactga gtaatatcta
     7861 taattatttt tatctttata ttcaggagaa aaaataccta ttttgtcata gaaaattaat
     7921 gtctgcttag atataccaca caatttagca aattcacctg ttgtaaaata ctgtttttcc
     7981 aagggttata cactcctttt ctttttgttg tctatatagt tactatattc tttattattt
     8041 actaaatcaa gtagttatta ttttagaaaa tcaatatggt tttgatttta aagcttaaat
     8101 atcagttaga tgtcagttat aaatatttta accattccca tcataaaaaa gatatcccct
     8161 cttaactttt ggatggaata ttatatttat acttgttaga gtgaaaattg taataggtgc
     8221 tacaataaga tatttaatta aacataaagt taattattgt aataaaaaat ctttagataa
     8281 attagagtta aagtatggaa atatagatag agaaaaagct gttaaactag aaatatttta
     8341 tcagtattta atagaacttg aatatataga aatgagttta ttaataagaa gattagatac
     8401 tgcaataaca tcattagcat tagtatctat tataacaata gcatcatatt atttaatcag
     8461 aaaaaatgtg taattatgta agctatttat aataaaacac tgataaaatt aataataaaa
     8521 aggtaatagg atttctatac tacctttttg tttttataat atagtgtaat tttattatat
     8581 ttttgtatgc taatataact agataatcat aagataacac attaaaagaa aattataaaa
     8641 acttacattc tgtatagtta atataaaaca taagtaatat aaatttatgt aaagcaataa
     8701 aagatggatt tacattccat atagttaata taaaacttta aatcatcttt atgaacttca
     8761 aaagtaggaa gcgtatttac attctatata gttaatataa aactagtaaa atcaaactat
     8821 gaaagaataa atgtttttaa tatttacatt ccatatagtt aatataaaac atcaatactt
     8881 tgatgcagtt tttaactagt ttttcaattt acattccata tagttaatat aaaactgata
     8941 agattcttgt tactgctgtt cgaaagttca aatttacatt ccatatagtt aatataaaac
     9001 gtttgaggat ttattagcaa ctcatagtga aaagtatatt tacattccat atagttaata
     9061 taaaactaaa gtttgaggtg agttttttga tttattttta ttcatttaca ttccatatag
     9121 ttaatataaa acaccaaaat aaacttagta ttttcaacac ttacacgtat acaactctct
     9181 taaatttgca gtgaacgagc aatagtgcaa ttgataacgc ttatcatgca ctctcaacgc
     9241 cttatatttc aactgttacg ccaaattttt tcacaaatat cgctcactgc aaaatttcta
     9301 taattttatt atatcataaa tatattactt ttgaatattg ataccaattt ataccataat
     9361 aaaagcaaaa aaacgtaatc tatctacatt agatttaaat tctataactt aaagtttttt
     9421 acataatatt tatgcttgtt taatcaatct aatatatcat ttttgtatat tgcttttaat
     9481 ggtctttttt cttaaataaa tatctatctg aacaagcata gtttaattaa aaacttttgt
     9541 attcccttaa aactgtatct acctcttatt attttattta tgatatatta tttttaatct
     9601 aaaaatagat atctattttt attatggtta tcccttttta aaatacttta atcatatact
     9661 attcccaatt taacatccat tgaaatgcta ttttcttata ttgcaattta taattgatat
     9721 atcttcaata taataattta atgtattttt gattttaaag cttttcagtg atattattga
     9781 taaattagaa ttaattatct agctttaaaa ggtcaaagat ttctgttgct gtctgcggtt
     9841 ttccaaataa atatccttgt atgtaatctg gctttatttg tttaactaaa tcatactctt
     9901 cttgtgtttc tacaccttct aaacatactt ttattcctac atcatgacaa attgcaacaa
     9961 taaaatgtat aaatgtagca tcaaacttac tctttaatat atctttaaca aatactctat
    10021 caattttcac tatatctatt ggagaaaatt ttaaaacttc tagagacgag tatccagtac
    10081 caaaatcatc catagcaata taaattccta aatcttgtaa agccttaaat ttaaactgta
    10141 aaatactcat attttgtact gtatgacttt ctgtcaattc taaaacaaga ttttgataag
    10201 gaaaattttc atcatctata atttttacta tatcttcaat aaatgtgtct tctaatattt
    10261 gaacagctga aacgtttaca ctcactttaa aaaaaggata gtaatcaatc catttcctaa
    10321 aagtacgtaa tgccattcga agcacccaag cccctacaat atttatcata tcattttctt
    10381 ctaatattgg aataaattcc aaaggagaaa ttgctttgca tttgtcatta gtccacctta
    10441 aaagaacttc tacacctatt attttatgac tttctgtatc tacttgtggc tggaatcgaa
    10501 gactaaaccc cctaaaatca tcattaattg atgcctttaa atcccgcatc atttctaatg
    10561 aatacatttt attttttaat atttcttggg agaaaaaaac aagcctgttt ttaccatgct
    10621 ctttcgcata ttgtaaagag taactagcac acttataaag ttcttttact gtatctccat
    10681 gttttggata tattacacaa ccagcagaaa ttgttatatt taatccatat tttctccata
    10741 gttgaaggtg tataatcata ttctgtattt gattatatag agttagaatt tcattttctt
    10801 caacattatc aacaagaatg cccatttcgt ccccatcaag tttataaagt tcagcattat
    10861 ctggtaaaat agattgtata gactgtgcta gtgtttttat tatattatct ccaaaatccc
    10921 tatcatacat ctcattaaca tttttaaaat cgtcaatatc taataatact atgcataatt
    10981 tttcaatcat aggatttgaa attttatctt caaatacact cataaattca tgataattca
    11041 gtaactgtgt caatgaatca atcttatttt gtttacctaa ttgcgtcatt attcctgcaa
    11101 aaatacttgg ttctccaaat tcatcacgca ttagttgacc tctacatctt aaccatatat
    11161 attctccact acgattttta gccctaaatt ccactgtatg ataatccatc tggtttttac
    11221 ctatttcagt gtttgactta taaaaacgat tccagtcttc tggatgtaca atttttttcc
    11281 agtaaataag cggattttca acaatttctc ctggtaagtc aaataattca acttgtgatg
    11341 gtgaatatcg aaatacgcct gttttcatat tgcaaatgta tatatattca tctgtacctc
    11401 taatcaaagc atcgtaaagc aatgtagaat tataatcaaa attgtctctt gaaaagttaa
    11461 aaactccttt ttcttcttta ttatttgata tttcatgaaa ttcattaatc ttaattggtt
    11521 tatttaagct tgttgagttt gcaatatcta ttgattgatg catatgaaca actgtgccat
    11581 cttgccatgt aattaaactg tcataatttt caaaaacacg atttaactta ttacattttt
    11641 cataccactt tattaatact ccttttttat cattctttaa taattctaaa actttacaaa
    11701 aactacaagt agaatttttt tcaggatata atacttgcca acaaactttt ccttctggat
    11761 ctaaaatatt atattcttct ttcatttttt tgttcataaa aataatttca ttagtatgaa
    11821 tatttgtaac atatatatta atctgtagtt gatttaatat aacttcaaaa ttatgtttgt
    11881 tcatatactt atctcctcca tagttactgc tctttataac aacattgtta aaattattct
    11941 ttttagaaga tttacttata acttatacat atacactaaa tacct
//
LOCUS       DAFKMX010000121        11918 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFKMX010000121 DAFKMX010000000
VERSION     DAFKMX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.121"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            166..837
                     /locus_tag="LZB08_002603"
     CDS             166..837
                     /locus_tag="LZB08_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316095.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            940..1488
                     /locus_tag="LZB08_002604"
     CDS             940..1488
                     /locus_tag="LZB08_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316096.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            1750..2058
                     /locus_tag="LZB08_002605"
     CDS             1750..2058
                     /locus_tag="LZB08_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316097.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(2220..4010)
                     /locus_tag="LZB08_002606"
     CDS             complement(2220..4010)
                     /locus_tag="LZB08_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316098.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(4010..5737)
                     /locus_tag="LZB08_002607"
     CDS             complement(4010..5737)
                     /locus_tag="LZB08_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316099.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(5982..6248)
                     /locus_tag="LZB08_002608"
     CDS             complement(5982..6248)
                     /locus_tag="LZB08_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBM7316100.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            6546..7658
                     /locus_tag="LZB08_002609"
     CDS             6546..7658
                     /locus_tag="LZB08_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBM7316101.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(8023..8532)
                     /locus_tag="LZB08_002610"
     CDS             complement(8023..8532)
                     /locus_tag="LZB08_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HBM7316102.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     SLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(8534..9181)
                     /locus_tag="LZB08_002611"
     CDS             complement(8534..9181)
                     /locus_tag="LZB08_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316103.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(9195..10367)
                     /locus_tag="LZB08_002612"
     CDS             complement(9195..10367)
                     /locus_tag="LZB08_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBM7316104.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            10635..>11918
                     /locus_tag="LZB08_002613"
     CDS             10635..>11918
                     /locus_tag="LZB08_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316105.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYNCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVN"
BASE COUNT         4184 a         1887 c         1489 g         4358 t
ORIGIN      
        1 ctgcctttaa tgttaatcat gcttcacact tcaataattt tttatatttt ttaatcttat
       61 ctgcaattta aattgcaaaa gattgacctt attttcacta tgttgtatgc ttaaaattat
      121 ataagttttt gtatttttat attgtttagg ggggtattat taagtatggc tacatttaaa
      181 agattacaag aacaagcata tgactactta aaagaattga tactatctgg agaaatgata
      241 gaaaatgaaa tatattctga aacaaaactt gctagtgaaa taggtatctc tagaacacct
      301 ataagagatg ctcttcaaag acttagtcaa gatggattta tagatataat cccaagcaaa
      361 ggatttagaa tccatcaaat tacagcaaat gaaattgtag agatattcca aatacgctct
      421 gcaatagaag gattttgtac ttttttaatt acaagtcaat acaaagaagc tagagcagta
      481 gaaactataa gtaaattaaa acatctttta gataaacaaa aagatatttt attaggtgat
      541 aaaaatttaa actcatttgc agaatacgat acattatttc acacaactat agtttcatat
      601 gctcaaaata ctgaatttga caaaatgttt aacaattata tgtacagaat aaaaaaatta
      661 gctctcgatt cattgtcaca tgaaggaagg cttgaaacaa cattaaaaga gcacacagat
      721 atttttaata atattgcaaa tggatgtatt gaagatattt acagaactac tctcattcat
      781 atggaaactc ctaaatacat taatttagaa gatttttgta acaataagtt attttagtat
      841 tatactttca tttattaaaa atatatctat ctcaaattaa tttcctaatt tataaaaaat
      901 attctataat tatattgtaa ctaatactta ttctaaggag tgattttttt gaacgaagat
      961 gttgtaacta atgaaactat tgacaaagat tatgctattg aagaagttcg tatggcatgc
     1021 aaacattttg gtgatttata tttttatttt tccaaagtgt tatttgaaga gtttggtgaa
     1081 gataaaacta gcgaaatatt aagaaaagtt ttatttgaaa ggtctgaaga aagagctatt
     1141 gctatgagag aaagagctca tgaaaatgga gatgaattaa ttgcagacaa tattattagc
     1201 acaactgatg ttccatttct aggatgggtt cctgaatttg aagagttaca ttgcccctat
     1261 ggagcttcct ggcttttaag atttgaggaa aatccttggt ttaaaaaatt cgcttctctt
     1321 tactgtgatg ttacagatac tactgtagct gaagtattta caggagatac ttcccataaa
     1381 ataacaaaaa acattctatg gggagataaa agttgtgaaa gaatatattt ccatgatgac
     1441 aaggtatctg aaggtaaata tacttatgga aataaaaact gtaaataaat aatttaaatt
     1501 ttctatattc atatactata ttttgtactt tttcatataa atattataac tagtctttta
     1561 ttttactttt gtttaaccta aagataattt acttttaatc tatatgaatt atcatataaa
     1621 taatcagaaa gttatcttta taaattatag ttttagattt ataatttata aaatatttat
     1681 gttctttgta aaatttttta gaagcttcta atgttatgct ataaaactat cttgagaagg
     1741 gctgattgaa tgtttaataa aatatatgaa aagaaagaat ttatagtttt tcaagttaaa
     1801 aaaggttatg tagtttataa tactagaaag agttttgaag aaggtcatac acatttaaag
     1861 cattttgaag cagcaaagac agctattgat ttagcaataa ataaaaagat tcctagaagc
     1921 aaagatggat attatctgac tagtttaatt cgcataagtg atgatggtta ttacattgat
     1981 aagttaagcg aattgttata tgttagagaa caaaaaggta aaaaagaaaa gtactgtaat
     2041 tctggttatc agatgtaaca agactagttg tgaattataa gtaccaatca taagtacctt
     2101 tagtcttaaa atcaaagagt atcagtaaat ctataaaaaa acaacttact gatactcttt
     2161 ttaattatat gattaatatt tgaatttgat acaaaatttt atacaaaagt ttatgttatc
     2221 tattctgatt ctttgtctgc aaattggcta ttataaagct tctcataaaa tccacccttt
     2281 tccattaatt catcatgatt cccctgctct ataatacttc cattattcat aactaaaatc
     2341 aagtcagcat tgcgaattgt agaaagtcta tgtgctataa caaaactagt tctaccattc
     2401 ataagatttc tcattgcttt ttgaagcata agttccagcc tagtatcaac agaactcgta
     2461 gcttcatcaa gtatcaatat tgaagggtct gatataaaag ctctagctat ggttaaaagc
     2521 tgtttttccc ctagagatat attagatgct tcttcattta aaaacatatt gtatccatct
     2581 ggaagagtgg ttataaagtg atgaacattt gctacttttg ctgcttgtat aatttcttct
     2641 ttagtagccc caaatctacc atattctata ttttcaaaaa tggttccatt aaataaccat
     2701 gtatcttgaa gcaccatacc aaacattgaa cgcaaatctt tacgtttcat atctcttata
     2761 tctactccat caattttaat agcacctttc tttacatcat aaaacctcat caataagttt
     2821 attaatgttg ttttacctgc tccagtaggt ccaacaatag ctaccatctg accacttttt
     2881 acttctgcat ttaaatcttc tattaaagtt gaattttctc catatccaaa attaacatgc
     2941 tcaaatgtaa catttccttt tacattttct atcttaactg gattttcaac atcaggtatt
     3001 tcctcttctt cgtctaaaat ttcaaataca cgttctatag ctgcaaaaga agattgtatt
     3061 actgtagata actgagtcat ttgtgaaagt ggctgattta tctgccatat ataacgcaca
     3121 aatgcttgta aatttcctac tgttattcct ccacttactg catatataga accgacaact
     3181 gccacagctg caattccaaa gtaagttact aatgaaacca taggattcat catacttgat
     3241 ataaattgtg ccttaaatcc attttcacaa agttcttgat ttactttttt aaattctttt
     3301 atagaatctt cttctttacc atatagtttt atttcattaa atccagtata catttcttgt
     3361 actttcccat ttaattttcc aagtgcattt tgttgcaatg aaaatctact ttgagatttt
     3421 tttactacaa atttagatac aaaataacta attggaatta taaaaagagc tataagagcc
     3481 atatatatat taattgtaaa catcattgaa agtgccaaaa ttatgactaa gattgagttt
     3541 acaatttgca taaaactctg ctgcaatgca tttgatattg tatctacatc atttgatata
     3601 cgacttaata catctccaaa actattacta tcaaaataac taataggcaa tcttcttatt
     3661 ttattttcga cttcatttct taaatctctc attgtgtttt gaatagcatt cgtaagtaga
     3721 aaacttgaag tatacgtaaa aattgcattg gcaaaatata tacatgctaa aatcaccaat
     3781 atttttgtta cataatcaaa atttacggaa gcccctggta ctccttttgc gatactaatt
     3841 acatctttag tcaattgtgt tattatcaat ccttcagttt taggtgcaag tgcattcata
     3901 gtagctgctg aaattataca tataatagct ataataaagg aaaatttata tttctttaca
     3961 aatggaagaa gtctaccaat tgttttctta aaacctttag actttttcat tattctaatt
     4021 cctccttttt taactgagat gttgcaattt catagtatat ttcacagttt tttaataatt
     4081 ctttatgagt tcccattcct acaacttgtc cttcattgag tactataatc ttatcagcat
     4141 caataataga actaattctc tgggcaacaa tcaatacaat tgcatctttt gtttccttct
     4201 tcaatttagc acgaagtatt gcatcagttt taaagtcaag tgcagaaaaa ctgtcatcaa
     4261 aaatgtatat ttcaggtctt cttataatag cccttgcaat agaaagtctt tgtttttgac
     4321 ctcctgatac atttgctcca ccttcactaa tcaattcatc aaactttcta ggtttttttg
     4381 atataaactc atatgcttgt gcaacttttg cggaatattc taattctgac tctcctgcct
     4441 tatgtttccc aaatctaata ttatttgcta tgctaccagt aaaaagcagt gatttttgag
     4501 gtatgaatcc tatctttttt cttaaagctt ttaaatcata ttctcttata tctactccat
     4561 ttatcttaat gctaccttct gttacatcat aaaatctagg tatcaagtta attagagtac
     4621 ttttcccact accagtactt cctataaaag cgactgtttc acctgttttt gcagtaaatg
     4681 aaatatcctt aagtacagaa gcttctccat ctggataggt aaaagttaca ttatcaaatt
     4741 ctatgatacc cttattttca gtatccttaa ttccattttc aggatttttt atcaatggtt
     4801 cttcatttaa tagttctttt attctatttg cagatacctc tgctcttgga tacataataa
     4861 ataccattga aaaaagcata attgaaaaca tggcatggaa aagatattcc aaaaatgcaa
     4921 ccaactgtcc aacttgaagc tctccaatat ttatcttctc acttgaaatc caaaatacag
     4981 ccaaaacagc tatatttaac agtaaaaaga atgctggttg agtaattgac ataagcttaa
     5041 atagtttctt agaaacatta gcataatatg tatttgtttt ttcaaatcgc tctgtttcat
     5101 aatcatcatt tccaaaagct ctaattactc ttattccagt taaattttct ctcgatataa
     5161 ggtttaattt atctagtcct ttttgttgtt ttgaagatat tgcctgagat acttttgcta
     5221 ttattgctac tccaataatt ataaaaggta ctgatatagc caatactata gaaagttcca
     5281 cactggttct aattgtcatt attatactta taatgaacat aactggagtt aataaggcag
     5341 tccttagtag tatattaaca aattgttgaa tttgaaatgc atcattcgtt gttcttgtaa
     5401 tcattgaaga aattccaaat ttattgtatt cagtatgtga aaattcttgt gatttctcaa
     5461 atatgtcatt tcttatatct ctggttacac ttgttgatac cttagcagaa caataaccca
     5521 acaatatact acctacaacc cctataatag aaataataac aatcactatt cccatttgtt
     5581 ttatgtaagc tatattctga tttgcaatac cattgtcaat tattttagct attatagttg
     5641 gaatccccaa ctcgactaat gcaaaactaa atatagccag tatgtttaat actattaata
     5701 acttataatt ctttaaataa cttaaaatta atttcatatt tttctccttt cttaattttt
     5761 gagatagtgt aatcatgtaa taatactaaa tttataaatg cttaatttta taaaaaaagg
     5821 agaaaagagt taacgcactc tcttctccag catattcttt ttaatacacc ctccgacaag
     5881 tcgcttatat gttaacatat ttgacaagta atgtcaattc atatagccaa aagtctttta
     5941 aatgcttcat ttcataacat tgtaacaaat tggcatgtaa tttatttttg ttttttactg
     6001 tattccaatt ttacaagttc tccaatatta ctagctttct taaaattcaa attagtctta
     6061 tctataccag acttaaatag tgaaatccct ctccccaata ttattggcaa tatatatata
     6121 taataatcat caattaagtt atctttcata aataatttta ctatatcgcc tccaccaact
     6181 atccaaatat cttttttatc cttctctttt ccaatttgct caattaattt tttaggttct
     6241 aaattaattt atagtgtgat tattctatgt aaacatcact tttttactca ttcaataata
     6301 ccctattaat aaatcctcaa actaatttaa ataaactttg cataaaatta aaaatatatt
     6361 atataaataa tttaaaacta cactaactta ttgaaaattc taattattac aaataaatat
     6421 tgctctctgt taacgctagt tctataatat aattataagc tattaatatt ataaagccga
     6481 tattactaat ataataatta taatacttat agttaattta cttttgttga aaggtggtaa
     6541 ataacatgaa aatttcagaa gtcgctactc caaatttttt gctagattta gaccagttag
     6601 aaaagaatat agataaaatt caacaaattt gtacaaataa taataaacaa ctttggccta
     6661 tgcttaagac acataaaagt acttacatag cgagacttca aaaaaatgca ggtgctagtg
     6721 gttttctagt tggaacactt gatgaaggtg aagctctgat aaaagcaggt tttgaagata
     6781 ttatgttggc ttatccttac atgggagaag taaatataaa tagaattgta actatggcat
     6841 caaaaataaa tttaatctgt gctacagata atatagaatg tgctaaggct tatagtgaag
     6901 ctttttctaa atcaaaaata gagtgcaagc tacttattat tgtagattgt ggcttacacc
     6961 gttttggggt tgaaccagag aaagtggttg aacttgctac tgaaatttct aagctagaaa
     7021 acataaagat tgttggtata agttcgcatc caggtcaagt gtatggttgt agttctccag
     7081 aaggtgttcc agatgtttgt gttcaagaag attcttctat ggagaaagca tacaaagctc
     7141 taactgataa tggatttaat atagaaattg tcgcttctgg tagtactcca acagttgaat
     7201 ctgaagctgc ttctaaaact ataactgctg taagacctgg aaactatgta tactatgatg
     7261 ctatacagat aggtttaggt tgtgctactg aagatatgtg tgctcttact gttgtgtgca
     7321 ctgtaatctc aaaaaatagc agtggatact atttaattga ttgtggaagt aagtgccttg
     7381 gacttgataa aggtgcacat ggaaatagta gtattgttgg ttatggtagg gtatgtggac
     7441 atccagaact tactattgat tcactttcag aagaagttgg aaaaattaaa gtaaatggtg
     7501 aaactgatat aaaaattggt gatagaataa gaattatacc taatcattct tgttccactg
     7561 caaacttgac aagtaattta ttaggtttta gaggagatac aatagaaaaa gtcatagaag
     7621 tagatattcg tgaaaattca aaacaaatca tcctttaaaa acagatttat tctgatatta
     7681 acatgaaagt atatttttct taaaattatt gttaataact aatgttaatg ttggataaat
     7741 aaaaaacata aattctagaa atataaattt aaaagaagag gctgtctcaa aatagagata
     7801 agcctctctt tcattgttga aattatgaaa atatacttga tttcatacaa aaagagtatc
     7861 tcatgaacta aaaagttcac tttgagatac tctctttttt ttaattaata tacacattaa
     7921 ttactatctt agtttattac tatatatcaa ttactattca ttaatccttg tatatattaa
     7981 ttcttatata tcatttatag ttaggttata attttatgtt atttacatta atcccataac
     8041 agtgagtaaa actcttacta ttaacatccc cactattgca tcaaatatag gaactgccaa
     8101 cagtaacatt ctatacttag tactggcatt tgctacaagc actattctgg cataatgacc
     8161 aactagagta cccattaaca tacatggcat aattaatatg gttgcttgtg gtgcagtgat
     8221 tagactttgt gcaaataagt ttgcagctgt agctgctcct gctgccttag caaagaatgc
     8281 tgctatcaaa actacaacag attccccagg tagtccaaaa atacccatta ctggtccaaa
     8341 aatatttcct aaaatatcaa ctaaaccagt taattgtaaa aactgcacta ttacataacc
     8401 cattatcatg gctggtaata tctgttcaac acctatgtaa aaaccttttt ttgctccccc
     8461 cataaatatc tcaacagcag ttttcttagg tttactattt acaattgcat ttccagtatt
     8521 ttgttgactc atactacaca gcctccttag ccttattctt tttatttctt acacctatga
     8581 ttaatctaac tagattagca ccaagtattt tacagaatat ctctataagg attattggac
     8641 ctaatgccaa taatgctatt ggtaaaagtg gagcttgagt atttattgtg ttcagtataa
     8701 cagctgaacc agcatattga tatgctacaa atatagttct ttcatcatct gttatttccc
     8761 cactttcaaa taattctttc gtcattattg aggctacatc tgaactagta aatgaagata
     8821 caaatgctat tcctgctatt ccaggtatac caagcagagg tctaagtaat ggattaaata
     8881 tctttgatgc tgctttcatt cctcccatag attcaactac atctataaga cctacagcaa
     8941 aacagacagt tggaatcaaa gtcagagcaa ataagaaacc atcttttgct cccgttcctc
     9001 ctttacccat aaagtctaca ccatctgcaa tagtaccaaa tgaacctgtc agattagcaa
     9061 aatctaatgc tttaaggaag ccttgactat ctttaaacac tcctgaaaat aatactatca
     9121 atacaaataa agataaatac cctttccatg ttaccttatt atctccagct gttacatcca
     9181 tataaaatcc ccctttatac ctcaaattta tctaagttta aaataactgt agaaattaat
     9241 ttcaccctct caaacataga atctactact gcatattctc ttgatgtgtg gttccattct
     9301 cctcttacac caaatgaaca gattgtagga acgtttgcaa tagttaaata acttgcatct
     9361 gaactacctc caagtctctt agcatttaat tctccaaatc cattttcctt tgacacttca
     9421 tttacaaatg tatgaaatct cataacatct tctgtagttt caaatggcat catttcactt
     9481 ataaaattaa cttctgtaga agttccttct atgtaagttt ctttacatat ctctctaact
     9541 tgttctttaa cattctccat ttcttctact ttttcaaatc taaggtctac ttttatacta
     9601 caatcttctg gaatagaatt ttccactctt ccacccttaa tcacacttac acttactgta
     9661 gttccaacat ctaaattagt caatttttgt atgcgtagta tcttattagc catttcttct
     9721 atagcatttc ttccaccttc aaaatcatta cctgcgtgtg tttctacacc ttttacatgt
     9781 atattacatc ctattcttcc ttttctacct acacacaaac tattatctac taatccagtt
     9841 tccatattaa aagcacaaag accacctttt gcttccctca atataacatc tgcacctgtt
     9901 gaatccttat gtcctatctc ttcatctcca gaaaatacta tttttattgg tctttctttg
     9961 tatcctattt tatttaatgc tttaataaca tataaggaaa ttattatacc acccttcata
    10021 tctaaaacac ctgggccata tgcttttcct tctattattt taaatggatt ctctccaaat
    10081 gtacctgttt caaagacagt atccatgtga cctgaaaaaa ttataggctt tttatttata
    10141 ttagaaccaa gtgtacctat caatgtacta ccattatctc caacatctac taactcgcaa
    10201 tctaaacctt ctttttcaaa ctcaaacttt aatctcttag ccaatttatt tacgctttct
    10261 ttacaatgtg tatagctttc catattaaca atctctttcc ataatgaaac tatttcttct
    10321 ctattctcat caacaaactt agaaatttca tttaaatatg tattcataat cttctccttt
    10381 attttcaaat aaaatgtatt tctttcctaa aacctaagtt tattttagga aatcttaaaa
    10441 gactagtcct taattacaaa atagcacaat tattcttgac agtcaacata tttttcagtc
    10501 ttttttatat tttctgattt ttctttcaat tttccttttg taaaataaat tgacgcgttt
    10561 tatctactat tataaaatga ggttataaat tgattgatta gttacaatac tctcgaaatt
    10621 ttaaaggagg ttttatgaaa tataaaattg gttttttgac aacatcttat tttatgtcag
    10681 ataagtttaa acaagctctt aaagaattaa atgatacttg tgaaattaca tttattgagc
    10741 tcaaagggga gaatcctttt aataatttac ctaatatata ctatgagcaa gtagataatt
    10801 ttgatggatt tgtatgcagt ggtatagttc catatagtga acttataata aatataaaag
    10861 acataaaagt tccattaaat tttttaaagc ttgatgaaag agatttttat aaatacttat
    10921 tcaaattatt aaatagccaa aaagatattg actttacaaa atcatttatg gattttttaa
    10981 gagaggacaa caattattat gatatatatt cattaataga ctctaataaa tgtccatata
    11041 caattaaaga ttttaatatc cctcaacctg tatatgactt taaaaaatta aatgataaat
    11101 tattggaaat acatttagac ttattgcgag ataataagat taacctttct tttactagaa
    11161 attacatcat tagttgtgaa cttaataagc ttggatataa ttgtatttac atggttccat
    11221 ctacagaatc aatcctgaat acatttaaat ctctggtgaa tgaaataaca ttacaaaact
    11281 tagacaaaaa taaatctgca acttgtatag ttacagtaaa tagttcagag tacataaatg
    11341 aagatttgat atttaaaaat gatgagatac aaaatcaaat atataacact atattaaatt
    11401 ctctatcaag acatggattt tatggagttc aggtcaaaaa aaatgatatg aaaatagaga
    11461 tacacactac taaagaaatg ttaaacaata tgaccaataa ctatacagat ttttttattt
    11521 cagaagattt aaaagaaatt aaatatagct taaatatagg ttggggcatt ggaaatacaa
    11581 atattcatgc agagcaaaat gctcgtcaag ctaatggtaa atctgcttct ttgaatggaa
    11641 actgtacttt tatagtaaat gatactaatg atacaatagg tcctttatat tcaaataaaa
    11701 attctaataa tattgaagat agctcaatag ccaataaagt tgcttctttt atacctctat
    11761 ctaatacaaa tgtatctaaa atcatgtgta tgattaatga tagaaattca aataatgtgt
    11821 cagcagaaat attagcagac tatatgaaca taactcttag aagtgctaat agaatattga
    11881 gtattttata taaagcagga attgcaacta ttgtaaat
//
LOCUS       DAFKMX010000122        11671 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFKMX010000122 DAFKMX010000000
VERSION     DAFKMX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.122"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..261)
                     /locus_tag="LZB08_002614"
     CDS             complement(<1..261)
                     /locus_tag="LZB08_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBM7316106.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYIL"
     gene            473..1174
                     /locus_tag="LZB08_002615"
     CDS             473..1174
                     /locus_tag="LZB08_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316107.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            1256..1864
                     /locus_tag="LZB08_002616"
     CDS             1256..1864
                     /locus_tag="LZB08_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBM7316108.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGFHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            2188..2652
                     /locus_tag="LZB08_002617"
     CDS             2188..2652
                     /locus_tag="LZB08_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBM7316109.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            3289..4305
                     /gene="gap"
                     /locus_tag="LZB08_002618"
     CDS             3289..4305
                     /gene="gap"
                     /locus_tag="LZB08_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBM7316110.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            4700..5869
                     /locus_tag="LZB08_002619"
     CDS             4700..5869
                     /locus_tag="LZB08_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316111.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            6249..10502
                     /locus_tag="LZB08_002620"
     CDS             6249..10502
                     /locus_tag="LZB08_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBM7316112.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            10651..11145
                     /locus_tag="LZB08_002621"
     CDS             10651..11145
                     /locus_tag="LZB08_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBM7316113.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            11261..11638
                     /locus_tag="LZB08_002622"
     CDS             11261..11638
                     /locus_tag="LZB08_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBM7316114.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         4756 a         1161 c         2085 g         3669 t
ORIGIN      
        1 taaaatataa aaatatacta ataaaaattg taaaaatagt ataaatggta taccaaatgt
       61 aaatttaatg tgtttagttt tatgtctaaa agaatacatt ccaattatag aacctatact
      121 acctccaatt actgctatag acataagagt agcttctttt attctccatt cgtttctaat
      181 tgctcttttc ttatctatgt acattgaaaa gaaagcaata gaattaatca ctataaaata
      241 tattagaata aaatttttca cagtcttgcc tccataaaaa tatattatac attataaaac
      301 aagatatttg ttttggaaag ttaaatttgt aattagtaca tataaaatat ttgatattaa
      361 ttggaaaatt taaaaaaatt aaacaattag caaatagggg gtatatttat aagacacaaa
      421 gtgataaaat ctatttagaa aataatgtaa aataacagga ggataaagca aaatgaaatt
      481 tatagtagaa aaagaagttt ttgataaatt agaaaatgta tgctttggtg tagtagttgc
      541 aaaaggaata gataatacta aagaaataga aagaataagc aaccttttag atataagtat
      601 agatagagta gaagattact tcaaagataa aaaagtaaag gaatcagaag aaattatacc
      661 atatagagag gcatttagaa gtctaggcat gaacccaaat aaatttatga gttcgataga
      721 agcaatgaca acaagggttg ctaagaataa gaaacttcct catataaatc ctattgtaga
      781 tttaggaaac tctatttctt taaaatattt acttccaatg ggtgctcatg atatggattt
      841 tagaaatgat gatgtatatg ttagattttc taaaaaaggg gataaatttg taccatttgg
      901 agaaactgat gttgaattaa tggaagaagg agaattaatt tattcagttg gagacatggt
      961 aaaaactaga agatggatat ggagacaagg agaagaagga aaaattacta atagctcaaa
     1021 aaatatattt tttcctatag atggttttac tgatgctaat ttagataagg taatgagtgc
     1081 aagagaagaa ttagcaaaat tgttaaagga aatatttaat tgtgaaatta aagtaggttt
     1141 tgtagataaa gataatcctg aaatggaaat ataataagtt gttgaatact tgttaagata
     1201 ttatatttac ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag taaatttgaa
     1261 aaaaactata ataaagaatg taaatccaat gattgaaaat aagtttatag gactttttga
     1321 aatagagtat aaaaacaaat tagatgaaga taaggtttgg atggtagcat caagaaaaag
     1381 tagtgagcaa ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag atgcagttgt
     1441 aatagttggg tttcataaaa gtagtaaaaa attaatttta ataagacaat ttagagtacc
     1501 tattaatgga tatatatacg agttaccagc aggtcttgtt gatgaaggag agtctataga
     1561 tatttccgtt gaaagagaac ttagagaaga aacaggactt actcttttag aaattaaaaa
     1621 aaataaaagt attgataaag tttatttatc tcctggaatg agtgatgaat ctgttgcctt
     1681 tgtttattgt atatgtgatg gtgatataac agatgaattt ttagaaccag atgaagagat
     1741 tgaagcgctt ttaatttcgc aagaagatgc aaaagagatt ttacaaagta atcataagat
     1801 agacacaaaa gcatttttga ttttacaaat gtttgtgagt ttaggagcta aattatttga
     1861 atagatatat agaaaagagg ttatatagaa gagtaaaata ataaagttta ttttacttgt
     1921 ataaattata atctcttttt ttatacaata aagtataatt ttgtgtattt ttaaataagt
     1981 gacttgactc ttaaattttc ataatatgtt ataaaccaat ttaagatact acaataatta
     2041 aaattccaaa attatttaac atatttgcaa ttaaaatgag aaaaggtaaa ggctgaatga
     2101 ttgaaaaatt tgatgtaaaa tagtagctta gtttaatact atagtttgta gggttaaaaa
     2161 ttagaaattg taagggagat aaattctatg aaaaaattag ttattatttt aattagtatc
     2221 attagtatgg ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata taataattta
     2281 aacaacaaag aatccggaca aacaaccatt gaaatggact tagataaaaa ttatgatact
     2341 tcagacccat ttgttaatgc acgtttgttt tgtgtgtcta atgatataga tgttttagac
     2401 acagagattt cttttaaaat ggatggagat agtggtattg tggaaataaa ggacaataaa
     2461 acagatgaaa ctttatggag taatacatgg catggaagcg ttgataatga tacattcaca
     2521 atttctcttt ctaatataca aaaagagaaa gagtatgcta tatgctttac tggaattaaa
     2581 ataaatcatg cagttgtaaa ggttactttt gaaagcaatt tagttaagga gaaagaaaaa
     2641 ccctcaaaat aaattccagc ttttctataa cttgatattc agtacataaa agcagttgat
     2701 atttatgatt gactgctttt tcttatcaaa aaggataggt tgtgcatttt tacttgaaat
     2761 agtttatgca catcaaaagt tggtgagcat gatattggaa atggtaagca tgaaatcttg
     2821 ataatacaag gaaataaatt taagttttca attaagtata tttccataat ttagttaaca
     2881 ataagctaat tattatttat attttgatat ataaataata attgtagcaa attttaaaaa
     2941 atcatatatt ttttgaacta aataaaaatt tatttttatt attttaaatt agaggagatt
     3001 cttgaagata tattattttt aaaaataagt atagtttaaa aattcaaaaa aaagatggca
     3061 aaatatgtcc caagggacat tgagtaaaat aaatgatttc agcttattta cactaaaaag
     3121 tacatactaa atgaggggta aaataaagaa aaatgatata ctaattgtct tttgataata
     3181 aaaaaacatg gaatttgatt atttaatcaa gaaaaagtat tgaacaaatg atttatttag
     3241 tgtacaataa tcatgtaaaa acatagtaat ggagggagac atttcataat gaaaattaag
     3301 gtaggaatga acggatttgg aagaatagga agagctgtat taagaatagc tcaagaagaa
     3361 ttaggagata atatagaaat tgtagccata aatgcaagag caacaacaga atctttggca
     3421 catttattta catatgattc atgctatgga acttttagag gtgaagtaga ggcaaaggat
     3481 gaagacactc tagtagtaaa ctctaaagag ataaaaatat taagatataa tgacccagaa
     3541 gaattacctt ggaaagaatt gggggttgat atagtaatag aatcaacagg attatttaca
     3601 caaagagaaa aggcagaaaa acatattaaa gcaggagcaa aaaaagtaat tataacagct
     3661 ccaggaaaaa atgaagatat aactatagtt atcggagtta atgagaaaga atatgataat
     3721 gaaaagcaca atataatttc aaatgcatct tgtactacaa attgtctagc tccttttgct
     3781 aaagtattgg acgaaaaatt tggtatagta aaagggttaa tgactacagt acattcatat
     3841 actaacgatc aaagaatatt agataaaagc cataaagatt tgagaagagc aagagcagcg
     3901 gctgaatcta taatacctac cactacagga gcagcaaaag ctgtgtcaag agtactacca
     3961 caattagaag gaaaattaaa tggtttttca cttagagtac caacaccaac agtttcactt
     4021 gttgatttag tgtgcgaact taaagaaaac gttacatctg agcaagttaa ctctgtgtta
     4081 aaagatgctg ctgaaggaga attaaaaggt gttttaggat attgtgacaa gccacttgtt
     4141 tctatagatt ataaaggaga ttcaagatca tctattatag acgctctatc cacaatggtt
     4201 atagaagaca atatggttaa ggtagtttct tggtatgata atgaatgggg atattcttca
     4261 agaactgttg acttagtaaa atatgtagct gaaaaattaa aataatatat atttcttaaa
     4321 gcaaatttta aaaaaatttt ttaaaaaaat tatataaaac taaaaaaaaa gattatgaaa
     4381 aatattgaag gaaaaatttt ttcataatct tttttctttc atgcaaaaag atacaagtta
     4441 caacttatat ttgcatatta ggtcgttaga gaagtatata tatatttagt aaggaatcat
     4501 actttttata aaatgaaaga taagatgtcg aaaacttgca taaatctgaa aaatttttat
     4561 tattaaaata ctgatttcca ttttaaaaat gataaaaaaa tgacaaataa agttgcaaat
     4621 agtaatactt ataagtataa ttaaaaaaga gaaaaggttt tgttaaactt tataacaaaa
     4681 aaagaaggga tgattcaaaa tggagaagtt aacagacagg agacaggtta aggcagtaag
     4741 catggaaacc ttcattttcc taatattact agtagttggt tttgggtatg ttggtagtat
     4801 tatgggagca ggaatgatgt tcaaagtaat aatgagtaca gcacatgctt tactattgga
     4861 gacagttttc ttaattatgg ctatggcagt tcttgcaggc gcattaagcg cactactttc
     4921 agagtttgga gtaatagcgt taattaataa aattttcgct gtatttatga agcctttata
     4981 tggactacct ggtgctagta tagcaggagc tataacaact tatttatcag ataatccagc
     5041 tattatacct tttgctaaag ataaaacatt cacacagtat tttaaacaat accaagttcc
     5101 agcattatgt aacttaggaa cagcctttgg tatgggactt atcttaacaa catttatgat
     5161 ttctcaaggt acagaatatg tacttccagc attaattggt aatttaggtg caattatagg
     5221 tagtgtcata agtgttagaa taatgttgac atttactaag aagttttata aatacagtcc
     5281 ggaagaagat aaagcaacag gaacattaga gaaaaaggat gaatttagag aaatcagaga
     5341 aggaaatgta tttcaaagag ctttagatgc aattcttgaa ggtggtaaaa tgggggtaga
     5401 tatgggaatg gctataattc ctggagtcct agtagtatgt acattggtga tgttattaac
     5461 ttttggacca tctacagacc ctgttacagg tcaagaagtt tatacaggag ctgcatatga
     5521 aggaataaaa ttacttccag ttattggaga taaacttgga tttatattag aaccattatt
     5581 tggatttaca tcaccagagg caatagcatt ccctataact tcattgggag ctgttggcgc
     5641 agcaatgtct ctagttccag agtttataaa gtcaggagct ataactccta atgatatagc
     5701 agtatttaca gctatgggta tgtgttggag tggatattta agtacacata ttggtatgat
     5761 ggatgcatta aatgctagac agttggcagg taaagctatt ctttctcata caataggtgg
     5821 tttatgtgca ggagcagctg ctcactttat atttacactc gtaggataaa tgtaatcagc
     5881 taagataaaa aataatatac aaaaaatggt tgttgatatg taataagaat ttacatatca
     5941 acaaccattt ttttctgact aaagataatt tataatacat aaacattaag tggatattag
     6001 agttaagtta tatttatgta aaaagtaaca aaaatctgta gaaatttatg acaaaattta
     6061 gtacactaac tatatgttct tatttaaaat ttaaggttag aattaggaat tacctagtag
     6121 agtaagcttt gattaataaa agaatcataa aataaaaata ttagtcataa agattataaa
     6181 taacttactt aaaataaagc catagaactt acaaaaatta aaaaagcata taaatatgga
     6241 ggggtacaat ggatgaatta tttcatatag gaattgatgt aggttctaca acggtgaagg
     6301 ttgtggtact taataatagt aataatatag ttcataaaga atatagaaga cattattctg
     6361 atgtaaaaaa atcagttaaa gaagtactaa atggtatata tgaaaaattg ggagatataa
     6421 atactaccat tataataact ggttcaggtg gaattggaat atcaaaaaaa ttaggtgtaa
     6481 aatttgttca ggaagttata tctagtacaa aatcaataga gtattttcat cctgagactg
     6541 atgttgtcat agaacttggg ggtgaagatg caaagataac atatctaagt ggaggaatag
     6601 accaaagaat gaatggaata tgtgcaggtg gcactggagc attcatagac cagatggcat
     6661 cactcttaaa gacagatgcc agtggattaa atgaacttgc taaagggtat aatgttatat
     6721 atcccatagc ttcaagatgt ggtgtttttg ctaaaacaga tattcaacct cttataaatg
     6781 atggagccaa tcagactgat atagcaatga gtattttcaa tgcagttgtc gtacaaactg
     6841 tgagtgtcct ttcttgtgga agaaaaatag aaggaaatgt tgcgtttttg ggtggtccat
     6901 tgtacttttt atctgaactt agagaagcat ttaaaagagt attagattta aaagatgaaa
     6961 acataatctt cccagaaaat gcacaacttt atattgctat tggagcagga ttattatcag
     7021 tagatgaaga taatatacaa ctaaaatctc taatagaaaa attggatagt ataaagaata
     7081 ttgaagatgg agaagtaaat ttattagaac ctttatttaa agataaaaat gagtatgaag
     7141 aattttcaag aagacatgaa aaagaaaaaa taaattatgt ggacataaat tcaataaaaa
     7201 gcaattgtta tttaggaatt gatgcaggct caactacaac taaagcagca cttattgatg
     7261 aggatggaag acttgtatat tcttattata atagcaatga aggaaatcca ttaaaaacaa
     7321 ccattaaagt tataaatgaa gtatatgata tattaccaaa aaatattaaa atattgagtt
     7381 caactgtaac tggatatgga gaaggtctta ttaaaaaagc tcttaaaatc gataatggag
     7441 agatagagac tatagcacat tacaaagctg ctaaattttt caacaaagat gtagatttta
     7501 tacttgatat tggtggtcaa gatatgaagt gcctaaaaat aaaagatgga gtaattgata
     7561 atataatatt aaatgaggct tgttcatcag gttgtggctc atttttagag acatttgcaa
     7621 gttctttatc tatgacaata gaagaatttg cgcatgaagg tatttattct caaaatcctg
     7681 tagatttagg ttcaagatgt actgtattta tgaattctag agtaaaacaa gctcaaaaag
     7741 aaggtgcaag tgttggagat atatcagcag ggctatctta ttcagtaata aaaaatgctt
     7801 tatttaaagt aataaagatt agagatttaa atgagattgg taataatatt gttgtacaag
     7861 gtggaacctt ttataatgat ttggtgctta gaagctttga aaaactaatt ggaaaaaatg
     7921 taattagacc taatatttca ggaattatgg gagcttttgg ttcagcactt atagctaaag
     7981 aaaaatatga aagtggatac aaaacatcct tgttatctaa ggaagagttg aataatatca
     8041 agctagaagc aagtgtaact agatgtaaag gttgttcaaa tcattgtctt cttacaataa
     8101 ataaattttc tgataatgaa atttttattt caggtaatag atgtgagaag ggtgaagcta
     8161 tttatggtga taaaaaaata ggattagaaa agaaaaaacc aataaattta tttaagtaca
     8221 aatacaatag aatttttaga tacaaaccat tggaaaaaga agaagcaaaa aatggggaaa
     8281 tagggatacc aagagtatta aatatgtatg aagattatcc attttggttt acttttttca
     8341 atgctatggg ctttagagtt gtactttcag ataggtcatc aaaacagtta tatgaaactg
     8401 gtataactag tattgcatct gaaacagttt gttatccagg aaaattagtt catggacata
     8461 tagaaaattt aattgcaaaa ggtataaaaa atatatttta tccaagtgta acaaatgaaa
     8521 ataaagagga ttcaaatgca gataattatt ataattgtcc tgttgtaata tcatattctg
     8581 aagtaataaa aaacaatgta gagaatataa gaaataagaa cataaattat ataaatccat
     8641 ttataagtct aaatgataag gaaaaattga agaaaagact ctatgatgaa ttatcaagac
     8701 atttttctga tataaaaata actaaagaag aagttaatca tgcagttgat aaagctactg
     8761 atgaacagaa tttatttaaa atggagatac aagcagcagg tgaaagagca cttaaagaaa
     8821 taaaagaaag aaatatgaag gggattgtgt tatgtggaag accatatcat atagacccag
     8881 aaataaatca tggaatgcca gagcttataa attcattaga tatggcagtg cttactgaag
     8941 actctatatg tcatttagcc aatgtacaaa gaccacttag agttgtagac caatgggtat
     9001 atcattcaag attgtataaa gcagctagtt ttgtaagaga taaaaactat ttggaacttg
     9061 ttcaacttaa ttcatttggc tgtggattag atgcagtaac tacagaccag gttcaagaaa
     9121 tacttaatga aaaatcgaag atatatacta ttataaagat tgacgaaggc aacaatttag
     9181 gagcagctaa gataaggatg aggtccttaa aagcagctat gtttgagaga gaaagcaaaa
     9241 atatagatgt aaaaaatata aaggtaaaca ggatacagta tgctaaaaat aatcagataa
     9301 cgaacaaaca tactatatta gcaccacaaa tgtctccaat acatttccaa ttccttgaaa
     9361 aagctgttaa cttaagtggt tataatatag aaattctaaa agatactgat agtagtgtaa
     9421 ttgaagaagg gttaagatat gtaaataatg atgcatgtta tccagctata atagttgtag
     9481 gtcagctaat aacagcatta aaatctggca aatatgattt aaataacact tctgttacta
     9541 taactcagac tggtggaggt tgtagagcta caaattatat tggattctta agaaaggcta
     9601 tatacgatgc tggatttaaa gatgttcctg tcattgcact tagtgtaaat ggaatagaag
     9661 atagtggtat aatggataat atatcactaa aattaatcaa tagactcttt atgtctgttg
     9721 tttatggtga tttgctgatg aaagtactat atagagttag accatatgaa aaagttactg
     9781 gaagtgcaaa tgctctatat gaaaaatggg ttgatatatg caaatcttct cttgttaaag
     9841 caaaaatttc tgtatttact agaaatataa aagatatagt aaaagaattt gataatttag
     9901 agatactaga tatcaaaaaa ccaaaagtag gtttggttgg agaaatattg gtgaaattcc
     9961 atccaattgc aaataataat ttagtagata tattagagcg agaaggtgct gaagcagtcg
    10021 tgccagacct tacaaatttc ttcttaagtt gtgcctttaa tactatatac aaacacactc
    10081 atttagaagg aagtagaaag agtagaatga taggagaggc atttatatac ataactggaa
    10141 tatatcaaag agtatataaa aaagcacttg ataagagtga gagattttat gctccagcca
    10201 atataaaaaa tgttgctaaa agtacagaac cagtagtgtc attaggtaat caaactggag
    10261 aaggttggct tttaacaggt gaaatggttg aattattaaa tgaaggtgta gaaaacatta
    10321 tttgtatgca accttttgga tgtttaccta atcacataat tggtaagggt tctataaaag
    10381 aattaaagag gttgtataaa aatgcaaata taatacctat tgattatgac ccttctgcaa
    10441 gtgaggtaaa tcaacttaac aggataaaac ttatgctttc taaggctttt aaaaatatat
    10501 aagatttata ttttgatatg ttaaaagtta atgaatttaa aaatatactc tatattaagt
    10561 atctataaaa aagagttatc ctatttttta gggtaactct ttattagtta aaaaattata
    10621 tgtatactga tattaaaggg gggaagtaat atgagtttag tatatgatgt ttataaatca
    10681 aaattaggaa atttgtacat attaagtgaa gatggtttta tagttagtat atacatagga
    10741 gatgaaaaat ttaataattt aaaaaatgaa tgtattaaaa aaggaattga tataaagaga
    10801 agtaaggaag atttaaagga agcaataagc cagatagatg attactttaa tggaaaaaga
    10861 aaagtcttta atttaaatat tcgtattgaa gggactgaat ttcaaaaatc agtttatagg
    10921 gaaatgttaa aaatacctta tggagaaaca ttgtgttatt cagatatagc aagaaatata
    10981 aacaatccaa aatcagtaag agcaatagga caagcaagta aaagtaataa aattccaata
    11041 ataatacctt gtcatagagt agttggcaaa aaagatattg gtggatatat ggggaatcat
    11101 tctgatttga aagagatact attaaattta gaaagacaga gttaacttag aaagaaatgt
    11161 ttttattgtt aatatttgat ataataatag taaaaagaag tattttttac aaaaacaatg
    11221 aagaatttat tttaatttac aaaaatgagg gggaatttta atgtgtgata aagttatagc
    11281 atcaaaatat catgagaagg gatttaattg tgcagaatca gttataaaag catataatga
    11341 ggaatttaat acagatatac cagtttgttt aggaagtgga ctgggttcag ggtgtggggt
    11401 tgctagttta tgtggtgcag taaatgcatc aaatataata attggatatg taaaaggcag
    11461 aaaccacgaa gatgaaagta ctaaagctaa agtatatgct aaagatttaa ctacaacagt
    11521 aagaaaagag tatggttcag agttatgtat agacctaaaa aaagatttag ttgcttgtag
    11581 agaaataatg gatttcgcat atgatagtct aaaggaaact ttaaaaaaag aattataaga
    11641 aaataagcta tcaaatataa attatttgat a
//
LOCUS       DAFKMX010000123        11248 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFKMX010000123 DAFKMX010000000
VERSION     DAFKMX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.123"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1033
                     /locus_tag="LZB08_002623"
     CDS             <1..1033
                     /locus_tag="LZB08_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBM7316115.1"
                     /translation="LLLFVVGCSNNPNKESTIKKDSKNNQSQVKEEKREPVKKDITIS
                     FAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDLTVVNLEGPLTTA
                     SNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDTQFILDENKINYF
                     GLGKDVVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIESLKKKSDVVVVYFHWGIE
                     LDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYSLGNFCFGGNKNP
                     SDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKILDGSEKDRVLNKL
                     EKISKDLNK"
     gene            1203..3104
                     /gene="cooS"
                     /locus_tag="LZB08_002624"
     CDS             1203..3104
                     /gene="cooS"
                     /locus_tag="LZB08_002624"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBM7316116.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3097..3570
                     /locus_tag="LZB08_002625"
     CDS             3097..3570
                     /locus_tag="LZB08_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7316117.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3545..4771
                     /locus_tag="LZB08_002626"
     CDS             3545..4771
                     /locus_tag="LZB08_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBM7316118.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSI"
     gene            complement(4910..6097)
                     /locus_tag="LZB08_002627"
     CDS             complement(4910..6097)
                     /locus_tag="LZB08_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7316119.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6370..7194
                     /locus_tag="LZB08_002628"
     CDS             6370..7194
                     /locus_tag="LZB08_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7316120.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVYKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7323..8588
                     /gene="gluD"
                     /locus_tag="LZB08_002629"
     CDS             7323..8588
                     /gene="gluD"
                     /locus_tag="LZB08_002629"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBM7316121.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8801..9391
                     /locus_tag="LZB08_002630"
     CDS             8801..9391
                     /locus_tag="LZB08_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBM7316122.1"
                     /translation="MHFKSKDFHLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYDLTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFTYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9721..10854
                     /locus_tag="LZB08_002631"
     CDS             9721..10854
                     /locus_tag="LZB08_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBM7316123.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYCTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
BASE COUNT         4419 a         1182 c         1973 g         3674 t
ORIGIN      
        1 tctattgcta tttgtagtag gatgttctaa taatccaaat aaggaaagta ccattaaaaa
       61 agactctaag aataaccaaa gccaagttaa agaagaaaaa agagaacctg tgaaaaaaga
      121 tataactata agtttcgctg gtgatgtaac tatggggaac tataaaggtt catcatatta
      181 tggcagtttt gaccatgaat ttaaaagaca aggagaagat tacaattatt ttcttaaaaa
      241 tgtaaaacct gtatttgaaa aagatgactt aacagtggtt aatttagaag gtccacttac
      301 tacagcgtca aatgcaaaag aaaaaaaatt tgcatttaaa ggaaagcctg gatatgtaaa
      361 tattttaaaa tctggaagtg tagaggcagt aagtattgca aataatcatt cagaagatta
      421 ttttgaagaa ggaatgaaag atacacagtt tatattggac gagaataaaa taaattattt
      481 tggattaggg aaagatgttg ttgtagatgt caaaggaatt aaagtgggtc tattagggta
      541 taatggatta tcaactgaat ataatgaaaa aaatttaaaa caaatggaag atgatattga
      601 gtccttaaaa aagaaatcgg atgttgttgt agtgtacttt cattggggga tagaattaga
      661 ttattatcca gataaaaaac aaaaagattt tgctcattat gctatagaca agggagcaga
      721 tttagttatg gggtctcacc cgcatgtgat tcaaggaata gaaaagtata aaaataagta
      781 tatagcttat agtctaggga atttttgctt tggaggaaat aagaatcctt ctgatacaga
      841 tagttatata tatcaacaaa cctttacttt ttcagataat aaattaactt caattaaaga
      901 gccaaatata ataccaactt caataacatc tagtaactcc agaaataatt atcaaccaaa
      961 aatattggat ggaagtgaga aggatagagt tttaaataaa cttgaaaaaa taagcaaaga
     1021 tttaaataaa taaaatgtaa aaaaaaataa atatatatat ttattttaat aatttatatt
     1081 ttgtttaggg tatggtaaat cgtggtatta aataagtaga ttttaatagt taaattatag
     1141 tcaattataa taaagctatt taatctactt ttttaatata aaaatataag ggggaatttg
     1201 taatggcttg taacacttgt aaaatgtgcg aaagtgcaga caagaaatta gaaagttttg
     1261 tagcttcaaa ggatgttgaa acagcatttc atagaacaga agatcagaaa gttaagtgtg
     1321 gttttggact tcagggggtt tgttgtagac tctgttcaaa tggaccatgt agagtaactc
     1381 ctaaatcacc aagaggtata tgtggagcag atgcagacac tatagtagca agaaactttt
     1441 taagagcagt agcatcagga gcagcttgtt atttacatgt ggtagaaaat acagctaaga
     1501 atttaaaaaa tgttggaata actaaaggtg ttgttaaagg agaaaaaaca ttaaatcaat
     1561 tagcagagat gtttggaata gaaagtgatg aaaagtatga taaatgtata aaggttgcag
     1621 acaaagttat caacgatttg tataaatcaa gagatgataa aatggaatta gtagaaaaaa
     1681 tagcttatgc acctagagtg aaaaagtgga aagagttagg tataatgcct ggtggtgcaa
     1741 agtcagaagt ttttgatgca atagtaaaat catcaacaaa tttaaatagt gacccagtag
     1801 acatgctagt taattgtttg aacttaggta tatcaacagg attatatggt cttacactaa
     1861 ctaatctttt aaatgatgtt atgttgggag aaccagttat aagaatggct cctgtaggat
     1921 ttaatgtaat agacccagac tacataaata taatgataac aggccatcaa cattcaacat
     1981 ttgcaaattt ccaagataga ttaaaagatg aagatgttat aaaattagct caatctgttg
     2041 gtgcaaaagg ctttaagcta gtaggatgta catgtgttgg tcaagattta caattaagag
     2101 gagaacatta tcaagaagta tttgctggac atgcaggtaa caattttaca agtgaggctg
     2161 ttttatcaac tggagcaata gatatagttc tttcagaatt taactgtact ataccagggc
     2221 ttgaaccaat agcagataaa tataaagtaa aaatggtttg tttagatgat gttgctaaaa
     2281 aagcgaatgc agaatacata ggtttagaca gaagtaaatt agatgaactt agtaacactt
     2341 taatagaaaa agcattggag tcttataagg agagaagagg aagtattgaa atagatatac
     2401 caaaagacca tggatttgaa caatctttaa ctggtgtaag tgaaaagaac ctgaaagcgt
     2461 ttttaggaga ttcatggaaa cctcttatta atttaattgc agaaggaaaa attaaaggtg
     2521 ttgcagctgt tgtaggatgt tcaaatatga cagcaggagg acatgatgta aatactgttg
     2581 aacttactaa agaattgatt aaaaaagata taatagtact atctgcagga tgttctactg
     2641 gaggattaga aaatgttggt cttatgtcac ctggagcaga agaattagca ggagaaaact
     2701 taaaagaagt atgtaagact ttaggaatac caccagtatt aaactttgga ccatgtctag
     2761 ctataggaag actagaaata gtagcaacag aattagcagc tgaacttgga attgacctac
     2821 cacaattacc attagtgctt tcagctccac aatggttgga agaacaagca ttagctgatg
     2881 gagcgtttgg tcttgccctt ggcttacctc ttcatttagc tttacctcca tttataacag
     2941 gaggaaaatt ggtgacagaa gtattaacag aaaaattaaa agacttaaca ggaggacatg
     3001 ttatagtgaa tccagaccct aaatcttcag ctaatcaatt agaagaaata ataatagata
     3061 gaagaaaaaa tttagggtta aaggatgtgg aatgcaatgc atagaatttt tataaataaa
     3121 gatttatgta caggatgtaa gagctgtgta ctagcatgta tgctaaaaca caataaagat
     3181 tatgacatgt acactcttga tttagaaaat atagataatg atagtagagg gcatatagaa
     3241 ttagatagta aacataataa tccagttcca attctttgta gacactgtga tgaacctgaa
     3301 tgtgtattag catgtatgag tggtgcaatg cataaagata gtgaaagtgg aatcgtttca
     3361 tatgatgaag agaaatgtgg ttcatgttat atgtgtgtaa tgtcttgtcc atatggatta
     3421 ttaaagccag atgatagaag caagcagaac atacttaaat gtgatttatg taaagatgag
     3481 gagtatccta gatgtgtagc taattgccca agtggagcaa ttgaattaca gaaggaggaa
     3541 aatgatgaat tatgtagtgt taggggctag tgcagctggt ataaatgcag taaaaacatt
     3601 aagagaatta gataaagatt caaatatagt agtaatttct aaagatgaaa acgtatattc
     3661 aagatgtatg ttgcatcatg ttatatctga acacagaact ttaaaacaaa taaattttgt
     3721 tgatgaagat tttatggagc aaaataatgt aaaatggatt gcaggtaaga ctgttaaagg
     3781 tatagacata aataaaaagg tagttcagac agaagacatt actgtaaatt atgataaact
     3841 tttaatagct acaggtgcat cttctgctat tcctcctata aaaaatttaa gagatggaaa
     3901 ttttgtatat tctgtgagaa acatagatga tatttataaa ataaaagaaa aagcacaaaa
     3961 ttctaaaaat gtagttataa taggtgctgg tttagttggt atagatgcat tagtgggatt
     4021 atttaaatat gagcagttaa atatttctgt agcttttatg gaaaagtaca ttttagacag
     4081 acaattggat gaatatacag catcagtgta tgaaaataag ttcaaagaaa aaggtgtaaa
     4141 attttaccca agtgcatcaa tacaagaaat tgtattggat aacagtaaga atgttacagg
     4201 tgttgcattt tcaaatggtg aagttttaga tgcagacatg gtaattgtag ctacaggggt
     4261 taaaccaaat gctgactttt tagatggaac aggaatagaa tatgatagag gtataataat
     4321 agatgatatg tgccaaacaa cacaaaaaga catatatgca gcaggagatg ttgtaggtaa
     4381 aaatgctatt tggcctctgg cagtaaagca aggaattgta gctgcctata atatggttgg
     4441 aaaagataag aagatagaag atgaatttgc ttttaaaaac agtatgaatt tcatggatat
     4501 accaactatt tctataggaa tgaatacacc tgtagatgat agctataaag tattaaccag
     4561 acatggaatt aatgattata aaaagtttgt ttttaaagat aatgtaatct atggagcagt
     4621 tatacaagga gatatttcat atgtaggtgt attaacttat ttaataaaaa ataaagttga
     4681 aatatatgat ttagaaaata ggatatttga tgtatgttat gcagactttt ttaatataaa
     4741 agaaaatgga gaattttgtt atagtatata gtatatacgt gaagtaaggt ttataaatac
     4801 agtaaatcaa taaatagagt tattctaaaa taagagaaat gacaaatctt atttagaaat
     4861 agctctattt tttgcaatta aatttaaatt atattcaatt tgtaaaatat tattttgaac
     4921 aattatacat atactctcta gttagaggta aatcattttt tataccttta gtgaaaagta
     4981 tttgatgtat atctataata ccagtgttga aagcagcagc acaagaacaa aggtataaat
     5041 cccacattct tataaactta tcatcaaaca tttctttaat ctttggaaga ttctcagaga
     5101 aatttttttg ccagtatagt aaggttttta cataatgtaa tcttagactt tctacgtcta
     5161 tagtatgata tttgtaatca ctacaaatat gtatcatttc tcttaggcta ggaataactc
     5221 ctcctgggaa aatatatttt tttatccaag ggtctccata agattcctgc aatccactta
     5281 tatagtgaag taaaaacaat ccaccatctt ttaaaacagc atttacgttt ttaatgaaaa
     5341 gttcatagtt ttctctacca acatgttcaa gcatcccaac gcttacaact ctatcaaaca
     5401 taaggtctga tttttttaat tctctatagt ccataagtct tacctcaaga tagtcttgaa
     5461 ggttctcttc tttaattctt gcgttaaatt ccttaaattg ctcttcgcta agtgtaattc
     5521 caacgccgtg aattttatat ttttttgcag cttctattag taaaaatccc caaccacaac
     5581 caatatcgag aaggctcata ccttcctcta aatttagttt agttaatatg tggtgaactt
     5641 tattgacttg agcatcataa agagtatcat tatcatttct aaagtatgca caagaataac
     5701 taagtgtctt atctaaccat aagctataaa aatcgttgcc aatgtcatag tgggcatgta
     5761 tttctttctt ttgatgtctc attgaatttg atgagtgcaa tagatgtttt acagctgaat
     5821 tatcaatatt gaatttgtct atttgtttta aaagtagatt taaagttaaa aataaatctc
     5881 cctcaacttc aatgtctcta tttatgtagg cttctccaag tgctaaagat gtacttttta
     5941 atagttcaga cttgctaata tcattattta ctataacttt gaactgaggg gagccctctc
     6001 caattagaaa ttcttcctca tctttaagcc taactagaaa tggtgtttca ccaaatttct
     6061 ctaaatagtc ttttaaaaaa ttttttgtaa tattcatatt accagctcct tctaatgtaa
     6121 atccagtaag tttccctgct taaaatatat tatagtcctt tttttaaaaa agtaaaattt
     6181 tttcaataaa ttttttattt aaaggaaatt tattttttgt aaagaaattt catgttgtag
     6241 tatatttttt gcttataaat tttggtactg gatataatat taaactgtta atgatataat
     6301 aaatttaatc tataaagtaa ataaaatttt gtaataaata gttaaagcta ttttataagg
     6361 agatatatga tggaagtata tgctttggtt gggtcaagtg gcacagggaa gagctttaag
     6421 gctttagagt tcgcatatga aaatgatata gaatatataa ttgatgatgg gattttaata
     6481 tacaaaaaca aggttttagc tgggatatca gcaaaacaag caaatactac tatagaagca
     6541 gttaaaaggg ctatttttta taatttggag catagacaag aagttagaga agcaattaaa
     6601 aaagaaaata taagaaaaat tcttattatt gggacatcaa aaaaaatggt taatcaaatt
     6661 gtagagagat tgtcaattgg aagggtttat aagtttataa atatacaaga tataagtact
     6721 aaatatgaaa tagaaatagc aaaacaagct agaaaagaag gaaatcatgt aattcctgtt
     6781 ccagcagttg agataaaaag tatggcaagt ggtcttagta taaattcttt gaagagactt
     6841 tttagaaaag gaaataatag gaatatggta gttttagaaa aaacaattat aagaccaact
     6901 tttagttata taggaaagtt ttatattagt gctgatgtaa ttaaacagat tattgaatat
     6961 gaagtataca actttggaag cattgataga ataaataaaa tcaatataga gaattgcaat
     7021 aattttatga atatttttgt aagtataaat atcaatgatt tagaaactat aaaaaaaatt
     7081 gaagacatac aacagacaat aaaaaagtct attgagaaaa tgactttagt taatgtacaa
     7141 aaaatagata ttaacattca taaaattaaa aaaataaact tcctccaatg ttaaaaaata
     7201 aactgagaaa atgatatact aatttgcaat aaagtgtgct ataattaaac tgtaaattta
     7261 ggaaatggtt ttttctaaat ggagtataca taattataac gtatttataa gggggctttc
     7321 aaatgtcagg aaaagatgta aatgtcttcg agatggcgca atctcaagta aaaaatgcat
     7381 gtgataaatt aggtatggaa ccagcagttt atgaattatt aaaagaacct atgagagtta
     7441 tagaggtttc aattccagta aaaatggatg atggttctat aaaaactttt aaaggattta
     7501 gatcacaaca taatgatgca gtagggccaa caaaaggtgg aataagattc catcaaaatg
     7561 tttcaagaga cgaagtaaaa gctttatcta tatggatgac tttcaagtgt tctgtaacag
     7621 gtataccata tggtggaggt aaaggtggaa taatagtaga tccatcaact ttatctcaag
     7681 gtgaattaga aagattaagt agaggataca tagatggaat atataaatta ataggtgaga
     7741 aagttgatgt tcctgcacca gacgtaaata ctaatggtca aataatgtct tggatggttg
     7801 atgagtacaa taaattaact ggacaaagtt ctataggtgt tataactggt aagccagttg
     7861 aatttggtgg ttctttagga agaacagctg ctactggatt tggtgttgct gttactgcaa
     7921 gagaagctgc tgctaaatta ggaatagata tgaaaaaagc taaaatagct gttcaaggta
     7981 taggaaacgt tggttcttat acagttctta actgtgaaaa acttggtggt actgttgtag
     8041 ctatggctga atggtgtaaa tcagaaggtt cttatgctat atacaatgaa aatggtttag
     8101 atggtcaagc tatgttagat tatatgaaag aacatggtaa cttattaaac ttcccaggag
     8161 ctaagagaat atctttagaa gagttctggg cttcagatgt tgatatagta ataccagctg
     8221 cattagaaaa ctctataact aaagaagttg ctgaatctat aaaagctaaa ttagtttgtg
     8281 aggctgctaa tggaccaact actccagagg ctgatgaagt atttgctgaa agaggaatag
     8341 ttcttactcc agatatatta actaatgctg gtggagttac agtttcttac ttcgagtggg
     8401 tacaaaactt atatggatac tactggtcag aagaagaagt agagcaaaaa gaagaaatag
     8461 ctatggttaa agcatttgaa tctatttgga aaatcaaaga agagtacaat gttacaatga
     8521 gagaagctgc ttacatgcat tcaattaaga aagttgctga agctatgaaa ttaagaggat
     8581 ggtactaaga tttactctta ataaataata ggatataatt atttaagata taaaggggct
     8641 gtacatagta cagcccttaa attatgtggc ttacaatgaa gtaagctatt tttgttttaa
     8701 cttataaaga acttgataaa ttatgtaatt tagaatgtat tttttaccac cacatgatta
     8761 caaattagaa gagttagatt gaatttttag acatgaactt atgcatttta aaagcaaaga
     8821 ctttcattta aagtatctag tattattttt aaaaatagta tattggttta atccatttat
     8881 atatataatg gaaaaatata tggatttgga ttgtgaactt tattgtgatg agagagtttt
     8941 aaaaaattgt actgtacaac aaaagcaaga atatgatcta actattgtaa attcaatgaa
     9001 aagaagtaca aatttttcaa ataaatttgt agctggatta aataaacaat ctgatattag
     9061 aaaaagagta ataaacatgt tcagtgaaaa acataaaaat ggtatcttaa gggctttgat
     9121 tataagcttg ttttcttcta ttacattttt taaattcgat tcaactagta ttattgaaga
     9181 gaatgatttc ttagagaggt caagatatac gtttgaagga aggcaagaag acatgtttgt
     9241 aacaatagat tttacatatg cagatgcacc tataaaactt agaaaagagc atgaagaaaa
     9301 ctgtagagtc aatggaatag taccaaaaga ttcagattca atagaaatta ttcctagatt
     9361 ttataaagat tgtatggatt taaaaaaata agttagactt aatattgatg ttgatattat
     9421 gaatttttta tatttaagca attgaaaatt ttattataga ttcaatttta tatgtgttta
     9481 agtatgaaat ttatagatag ttgaaataat taaatatgat tgatgcatag gaatcattac
     9541 taagctataa ttaattatag gggagtttaa ataaattatg agttacacaa ttataaaatg
     9601 ataaaaatag ttatctgtta caaaaaatgt atctttttaa ttaataaaga taatatgaaa
     9661 acttaaaagg caaataaact taaatataaa atactcttaa ataagatagg agaaaaatgt
     9721 atgtctaact ttattctaaa tgggattttt agaacagtta tagtaagtag tctaagcatt
     9781 cttctgatat tgattttcaa gaaaaatgta tttaaaagat ttagcaaaaa atttaattat
     9841 tatatatgga tgatagttgt tattaaatta tttttaccat ttacatattg tacttttaca
     9901 ataaatatct tgagaagtaa aaaacacatt aatattaata acataaattt agagggattt
     9961 aataatgttt caactataag taataatatt atattgtaca cttggattat aactgtagta
    10021 gtttatttaa tttacactat atttaaatat ataaaactta aaaatttaat aaatgactta
    10081 tcttatgatg ttgatgatgg agagattgta aatctttata aaaatatatt agaagaattt
    10141 aatataacaa aagatataaa actcaaatac tcttatgaag ttgaaacacc agcatttttt
    10201 aattcatgtg tgcttttgcc accacatgag tataaattga aagagttaga ctggattttt
    10261 agacatgagc ttatgcattt taagagtagg gacctttatt taaagtatgt aatcctgttt
    10321 ttaaaaacag tatattggtt taatccattt atatatataa tggataaaca tatagattta
    10381 gattgtgaac tttattgtga tgaaagagtt ttaaaaaatc gaaattcaga ggaaaaaaag
    10441 gattatgctt taacaattct aaatgcaatg agaaaaggtt caaatacttc aaataaattt
    10501 atagctggac ttcataagca atctgatatt aaaaagagag ttatgaacat gtttaatgaa
    10561 aagtacaaga atggtatatt aatggcatta actttatgtt tattatcttc tattactttt
    10621 ttgaaggtta attcaactag tattgttaat cttaatgtat tgccaaaagg aatggaagaa
    10681 aaagatgtta ctaatagtgc aattgttaca atgatagaat ttacatatgc agaagctcct
    10741 aaaagtatga gagaagaaca taaaataaat tgtgaattct taggtttaat acctaaagac
    10801 tctgataaaa taggtgttgg tatagaatat tatgacagtc tattgaatga ttaaataaat
    10861 attattttaa cagatattaa ttataaagtt ttgaatagta atttgttgag aaataaatat
    10921 atccttataa aaatagatac ttatatacca agaataaaaa atagttaaga tagtacacaa
    10981 ggatatactt ataaagaact taaataagtg aaataaatta ttaattataa ggtttataat
    11041 tttaaaaaat tagataaatt caggtgtaaa atactataaa acacaattcg gaaaagtgta
    11101 tgttgaactt catttaaaat ggaatttcaa agttgctata gtaagtagtt taagcattct
    11161 tcttatatta atctttgaga ataatgtatt taaaaaagtt agtaaaaaat ttaactattg
    11221 tatattaatg atagttgtta ttgatata
//
LOCUS       DAFKMX010000124        11180 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFKMX010000124 DAFKMX010000000
VERSION     DAFKMX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.124"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            326..991
                     /locus_tag="LZB08_002632"
     CDS             326..991
                     /locus_tag="LZB08_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBM7316124.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSNELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            1013..1945
                     /locus_tag="LZB08_002633"
     CDS             1013..1945
                     /locus_tag="LZB08_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBM7316125.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1966..2607
                     /gene="pcp"
                     /locus_tag="LZB08_002634"
     CDS             1966..2607
                     /gene="pcp"
                     /locus_tag="LZB08_002634"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBM7316126.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2727..3059)
                     /locus_tag="LZB08_002635"
     CDS             complement(2727..3059)
                     /locus_tag="LZB08_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316127.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3324..3944
                     /locus_tag="LZB08_002636"
     CDS             3324..3944
                     /locus_tag="LZB08_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7316128.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4259..5083)
                     /locus_tag="LZB08_002637"
     CDS             complement(4259..5083)
                     /locus_tag="LZB08_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBM7316129.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5094..6497)
                     /locus_tag="LZB08_002638"
     CDS             complement(5094..6497)
                     /locus_tag="LZB08_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316130.1"
                     /translation="MKWKITRNFIFIIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDDRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6501..7226)
                     /locus_tag="LZB08_002639"
     CDS             complement(6501..7226)
                     /locus_tag="LZB08_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316131.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7338..8111)
                     /locus_tag="LZB08_002640"
     CDS             complement(7338..8111)
                     /locus_tag="LZB08_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316132.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8117..9004)
                     /locus_tag="LZB08_002641"
     CDS             complement(8117..9004)
                     /locus_tag="LZB08_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316133.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(9018..10040)
                     /locus_tag="LZB08_002642"
     CDS             complement(9018..10040)
                     /locus_tag="LZB08_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316134.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            10615..10791
                     /locus_tag="LZB08_002643"
     CDS             10615..10791
                     /locus_tag="LZB08_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316135.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4014 a         1675 c         1275 g         4216 t
ORIGIN      
        1 aaaaagagag tgtctaatga actaaaaaat tcattagaca ttctcttttt ataatataat
       61 atctaattta aaacctattt caaatattga catatactta aactatagac ttacaaaatc
      121 aattaatatt agcttaaaat ataatctttg ttattcaaat tcctatacac aaccattatt
      181 tactttgata ctttaaaaca tcttccctat tttttattaa attttataat aatagtccta
      241 ttaacgcaaa tactataaaa gagtttaact ttatccatta aataaattta aattaaaaca
      301 tatagaaatg gaaggagaac aatttatgat aaaactaatc ggtatattga tagttgtaat
      361 tggatttata cttaaaattg acacactatt tactgtatta ttagcaggag tggcaacagg
      421 aattgttgca ggattagatt ttaatcaaat ttttacaatt ttaggagatt cttttgtctc
      481 aaacagaggt gtatcattat tcatattaac attgccagta atcggtgtat tagaaagata
      541 tggtttaaaa caaagagctg tttctttaat agaaaaatta aaaagactta caacaggaaa
      601 agtacttact atatacatga tagctagaca aatagctggt gctctatcaa taagaatgag
      661 tggacatcct caatttgtaa gaccacttgt taatcctatg gcacaagctg ctggtttaag
      721 taatagtaat gaactaaaag aatcggatga agaagccata aaggcattat ctgctgcatc
      781 agaaaactat ggaaattttt atggtcaaaa cttatttgca ggtagtagtg gagtattact
      841 tatagcatct actctaactc aatttggata caatgtaact ggacttaata ttgtaaaggc
      901 tagtactatt atggcagtaa tagcacttgt agtggctaca atccagttta ccttatatga
      961 taaaaaatta aacaaaagtc ataaaaaata gttttattta ggaggaataa aaatgcaaaa
     1021 ttttataaac atatcattgg aaatctttta cgcactaatg ggatttttaa tgattgttat
     1081 agcatataag tcttttacta ccataaataa caataagaaa tatggtacat cattattttg
     1141 gatacttata tcactgccat tcatatttgg tagattgatt ccagccaata taataggaat
     1201 aatattaata ttatctagtt tattaacact ttctaaacaa gttgtatttg caaagtatga
     1261 agaaccagat gaaaattttg gaaaagaaca agcagacaaa cttaaaaata aaatatttat
     1321 accatcatta atattagcat ttgctgcagt agtagtagct atgagccttt ctaattttga
     1381 taattcttct caatttgcaa taggtgtggg ttcaataatt gcattaatat cagcgcttat
     1441 aataactaag gcaaaacctg ctacatcagt tcaagatggc tcaagattac ttcaacaaat
     1501 gggacctgct agtatgttac cacaactttt agttgcattg ggagctttat ttactcaagc
     1561 cggagtcggt gaagttattt caaccatgat atctggtgta gtccctgctg atagcagatt
     1621 atttggtgtt atagcttatg tattaggtat ggttatattt accatgataa tgggaaatgc
     1681 atttgccgca ttttcagtca taacggcagg aattggtatt ccttttgtat tgtctcaagg
     1741 tggaaatcct gctataattg gagcattagc attaacagca ggatattgtg gaactctact
     1801 tactccaatg gctgctaatt ttaatatagt tccagcagcc ttgctagaat gtaaaaatga
     1861 ttatattgtt ataaaatatc aagctccagt tgctttggta ttaattatag cccacatatt
     1921 agtaatgtac tttttaggat tttaatataa ttaggaggta aaataatgaa aattttgtta
     1981 acaggatttg acccatttgg cggagaacca ataaacccag ctcaagaagc agttgaaagg
     2041 gtcaataata atataaacgg tgcagaaatc ataaagataa ctataccaac agttatgaca
     2101 aaatctgttg aagctataga taaagctatt caagagcaca atccagatat agtaatatca
     2161 gttggacaag ctggaggtag atttgacatt acacctgaga gagttgctat aaatatagat
     2221 gattttagaa taaaagataa tgagggtaac caggttatag atactattat aaaagaagat
     2281 ggtgaacctg catatttttc aaaattgcct gtaaaagcta tggtaaaaca tatgaatgaa
     2341 aataaaattc cagctagtgt ttcaaacact gcaggaacat ttgtttgcaa tcatgtgatg
     2401 tatggaattc tttatatgat agacaagaaa tatcctaata taaggggtgg atttatacac
     2461 atcccatata caacttcaca agtaatagat aaaaaaaata caccattcat gtctttagaa
     2521 gaaatagtaa aaggcttaga attagctata gaagcttgta ttatatataa agaagatgta
     2581 aaagaaattg gtggtgaaat ttcataataa attttttagt atttttataa attaaaaata
     2641 ctaactatta atttaaatat agactgtgag tagattattc aaacaatttt actcacagtc
     2701 tttttatggt atctacagat acaaaattaa ggtctattcc tctctcccca ctcacacatt
     2761 gcatctaaaa ttggaatcag agattttccc ctttctgata gactatattc taccttagga
     2821 ggtatttgag gatattcagt acgaataatc aagttatctg cttctaattc ttttaatgat
     2881 aaactcaaag ttttatagga gatagtattt atataacgtt ttaatgcatt atatcttaca
     2941 acttcaaatt ctacaagaca gtaaagaatt atcatcttat atttaccact aatcagtgaa
     3001 agtgtataac taaatcctgt ttcacttaaa tctgtatttg atatacagtc tatactcata
     3061 tattacactc tccttttggt tagtattgaa caaaaatgtg cgtacttgtt tttagaataa
     3121 tttataataa aattatagtg taagaataat tgaaagtcaa ttttataata ggaggtgtat
     3181 ttactaaaaa aaagttattt taaataggat tctaagatta gacatttcta tataaattga
     3241 taatattcat gaaaatccta ttttataaaa aataatatct cttagaaaat ctattaaata
     3301 aaattcattg gaggaaattt ataatgaaag ttttattgat aaacggtagc ccaaaccaat
     3361 atggatgtac ttatacagca ctaaatgaga ttactaaagt attgagtaaa cacgatatta
     3421 aaacagaaat actgtattta ggtaaagaaa caatcccagg atgtattagt tgcgcatcct
     3481 gttttgaaac tggaaaatgt attcgaaatg ataaagttaa tgaattgatt gaagatctgg
     3541 ataatattga tggtattatt attggttctc ctgtttactt ttctagtgca actggtcaat
     3601 taacttcttt tcttgaccgt ctatttttca tagctgggag tcgcatggct acaaaattag
     3661 gtgcttcagt tgtgtcatgt agacgtggtg gtgcaagtgc aacatttgac caactaaata
     3721 aatatttttc aatcagcaat atgcctattg tatcatcaca gtattggaac caggtacatg
     3781 gctttactcc agaggatgtt atgaaggatg aagaaggatt acagactatg cgaactctag
     3841 gagaaaacat ggcatggctt cttaagtgta tatcagcagg taaaaaagct ggaattaaag
     3901 aaccacaata tgaagaaaca attatgacta attttataaa ataaataaaa aatgtactaa
     3961 tattaattaa aacaaaacac taatttataa ataaaataag cataaatgta ggataaaaag
     4021 cactaaaaca gaggtactga attttatatc aaacctctgt tttattatta cattaataac
     4081 caaaccatgt ataacatcat atatttctaa ataaattata ctaatgttat tgaaattaca
     4141 ataactgaat ttctgctcat aaatttaaat attctacaaa tctcatctaa ataacaattt
     4201 taatagacct aaaacaatcc tcatattgtt cattttgtat taaaaatatc acttacaatc
     4261 aaaaaccatt tattaagtat attcctaaga tatacaatat atatacatgg attggatagt
     4321 ataaataaaa taaatacttc atactctttc ctttttctcc attgtaaagt aacattaaag
     4381 gtagagaagc aatcatcatc cattggtaat tttgtaaaat agaatttttt aaactataat
     4441 ctccatttga cattgttgta aatataaata tacacattaa aacataacaa atactcaaac
     4501 tgaacttttt acctctacac aaataaaaaa taatcccaag tataatccaa attggtccac
     4561 cttctactaa aaatggtact ggtaccaaaa tcataaataa agcaataact ctgttagtga
     4621 ctttaaaaag taaagctact gtaaatattc ctaaaattat aggaatgatt atcatcaata
     4681 atcctataat aaaatattta atttctttct cttttctaaa tttctttatg aactctattg
     4741 cttgtaaata aatcgctatt aaaaataatg ttgaaaaaat gttatttata attatagctc
     4801 cttctggatg tgaaaataca ttattaatta tttgatttcc tatagccata ataacagacc
     4861 aaatatacaa tctacttata tatttttttc tgttactagt gtgataaaat ccttcaacag
     4921 ccatgaatat aaacagtggt gctgatattc tgcctaccca atgaaaccaa attggtattt
     4981 gcattgtttg tggcataaat gctgctatat ggtcaaatgt cataaatatt aatgctaaaa
     5041 tcttaatagt aaacccactt ataccttttt taaaaaaatc catatcatat ctcctaattt
     5101 tgttttatct ctataataat ttttgttcct attccaattt cactacttac ataaatttta
     5161 cctttatgaa tctctattat ttctttagaa atagccatac caagacctga acctttatgc
     5221 atctcacctg tattagtacc tctataatat tttttaaaaa tatgtttcaa atcacttttg
     5281 cttattccaa ttccattatc ttttattata atttctatat tatctttttt tacaatttct
     5341 acacttatca aagttccttc gctattatga actattgaat taaatataag attggttata
     5401 gcccttctga ataagattga atctacatat acttcaatta aatcttcatt actttcaaat
     5461 tcaatattcc tatttttata tctatcatca tttaaaatat caattattat atttctgact
     5521 aaactgacta aatttatttt tttcttgtcc agaactatag tattattttt taatcgagta
     5581 gataaattta aatcatcaac taaatctttt atatacttag acttattata tataatttct
     5641 gtatactctc gtatttcatc ttcttcaaac tcataatctt tgtcattaat tatctctgca
     5701 tacccttgta tagatgctag aggtgtcttt atatcatgtg ttatatttgc cagccattcc
     5761 tctctcaact cttcattttc ttttctctca acttcattta ctcttaaagt atcagctaac
     5821 atatttatat ttttaaaaac ttcttcatat atacctctgt ccttaagata taaactataa
     5881 tttccattag ccatagtctc aatactccat agtacgcttg aaattggttt tgtaagtttt
     5941 ctactaaata aatatcctac gcctaaagct accacgctat caattactag tgttataatg
     6001 ataactttat ttaaagtttt tttaatgtta ttttgagaat aggttaaaat tattcttgat
     6061 atatctctac taggtatacc tactattata tttaaatgct ttttgtttaa atatttttct
     6121 agtacaaaat taacatactt tgtttctata tacttgtaat tattcaccat ttgaaagggt
     6181 gtatattttt ttggagtatc ctttggcaca tttactccat aaacttcctc tcctaaatca
     6241 tttaaaacct gtatccagga atttgatttt tctaattttt cagctccaat cttagatagt
     6301 ttaaattctc catctttttc atataaatct ttttcaaaag aacgtgcaaa ttcttcagga
     6361 ttatttccag aatctacaac tttaaaaaag ctattagttg aaatcacata caatatagat
     6421 atgatattta taattactac tgatatagct acaaacacaa ttataaatat aaaatttcta
     6481 gtaatcttcc acttcatatt ctaatcttcc tttactgcta atttatatcc aagacctttt
     6541 attgtcagaa tatattcagg ttttgaagga ttatcctcta atttttccct aatttttctt
     6601 atatgaacca ttatagtatt gtcaaatcca acataatctt ctccccacac tttatcccat
     6661 agtttttctt tacttataat ttgattttga ttatgtgcaa ataaagatag gatttttagt
     6721 tcctttgcag acagtcctat agattttcca tcttttataa gttctgccct actttcattt
     6781 aatatatatg ggccaaatga tataattttt tcttcattat tatttagatt tttactttcg
     6841 ttttgagcat cactaaaata acttaatctt ctcaaatgcg ctttaactct aaaagccact
     6901 tccttagggc taaaaggttt tgtaacataa tcatctcctc caatcgctag tccaagtaac
     6961 ttatctaatt cttcggtttt agctgataaa aatattattg gagaattgga ggtttttctt
     7021 atttctttac aaatatcata accttcgcca tctggtaaca taatatccaa tacaatcaaa
     7081 tcaggattaa tacttttaaa taattctaat ccttcttttt tagtttttgc agtatatata
     7141 ttattaaggc cttctttttt taggacagtt tctaaaagct tcaatatttc tacttcatca
     7201 tctattatta aaacacgatt ttccataaac aacctcctgt aataatttac atatatatta
     7261 gcatatattt tatctaaaat tcatttatct tatttttaat taaactaaaa tataaataaa
     7321 attactatta tttttagtta aaaccttttg cttgatgtaa aattttcata tgttatttta
     7381 gttaacacta tcattaatat aattgagatt actatagaaa aaactatcaa aatacttggt
     7441 acattatact taaccatttg ttggctcatc ataatccaat ttggagtcac tgacataaat
     7501 tttatagctt tatctggtat aaaacctaca ctaaagtatg gaattgccat tatcaatcca
     7561 cctagtataa atggaattac agccgattta aatatagatg aaagtgttaa gcctatttgt
     7621 gcaaatataa aagaagcaaa aatagtagtc aatataacaa ttatatacat ttgtattact
     7681 gtaagattta aattactatt tccaaagtac cataagttat gaatcggttc atttaaattt
     7741 ttaaaatcaa aaaaactaaa acctctaatt cctactaaca atataaaaga aatattaata
     7801 atagataaaa ttaatccatt agctactagt tttgataata ctactgattt ttttcccttt
     7861 ttagttgtat ttattaaacc tgtcatatta gaatcatatt ctttaatgta atttccagaa
     7921 gttattataa acattatcaa acctattaga ggtattgagc cttctatctt gtaaaatata
     7981 tctgcataag tatctaaaac atcaaacttt ctaatcagaa aaaatccaaa aaatattaaa
     8041 aacagccaaa ttatccaaac actctttgat tttaatattt ttatcatttc agctttaact
     8101 aaatacatca tttctactac acctcccttg catcttctaa atcaaaataa aacatgtata
     8161 catcttcgaa ttttgcttga actggttgta tattatcctc gcttggaata tcctctgaaa
     8221 caattctaag tactattgaa ttaatatcac tttgaatact tacaacctta tatttagatt
     8281 gtatatcatc aacttcacat tcatttttaa cttgaacatt atatacttta ttttccatgt
     8341 gacttaatat gtctttgtgt gttcctttca ttaggagttt gccatcttta atcattattg
     8401 tttcctttgc aactgattct atatcggaaa ttatatgagt agaaagtatt attatcttat
     8461 ctctagacat ttttgctatt aaattcctaa atctagctct ttcttgtggg tcaagacctg
     8521 ctgttggctc atcaaggact attatcttag ggtcgtttag taaaacttgt gctatcccaa
     8581 ctctcctctt catacctcct gaaaattttc ctatagactt atttctcaca tcataaagac
     8641 ctactaaatc taataattct cctatcttac tatttaaata ttttcccctt aaacctttta
     8701 aagctccaat atactgtaaa aattcttttg ctgtaaaatt tttataaact ccaaattctt
     8761 gtggcaaata tcctaaaata tttctataat cagcatccat tttaaatata tcctcaccat
     8821 tataaagtat ctgaccttca tcaggtttta tcaacgttgt tatttgtttc attaatgtag
     8881 attttcctgc tccatttggt cctagaagac cataaactcc attttctaga gttactgtaa
     8941 tcttgtcgtt tgctactttt ttaccatata tctttgaaag attttctatt tctaatttat
     9001 tcatcttaac tcccctttta tctacttata taatctgatt ttattactaa aaaacataat
     9061 gacatagcac ctatcaatat tgccatcaaa agtcctattg ctatattgac aatgtctata
     9121 ttcactttaa atatcataat atcacctaga taatttccta ttattttatt aaaaccaaat
     9181 atcaaatcat aataatttac atttgataca actgaaagta attttttagg taatatatgg
     9241 attaccgaca ttatcttagt tatagatata ataaatataa aaaaacttat tgaagatatt
     9301 gaagacttac ttataaaaga cccaaatatg gctatcatgg cccatccttc aaacatcaaa
     9361 agctctatgc ctatatttaa tattacatac tttgttatac ttaaattagt tttcaatgca
     9421 actacattgt ctactattct aaaagattct aagtctccat taacaggtac tccttgttgc
     9481 atacaagtta taataaatat tcccattaaa taaacagaat aaattactaa tggaactaat
     9541 aaagcaacaa atattttaga ctttaatgct tttttcttct cttttgatga tagtatgata
     9601 tcttttaccg aagtaacgac ttcatctacg taaatattag atataattat agatataatg
     9661 attaacattc ctacatttat aagtgaattt gtaactctat agttaaatac tttccaaaaa
     9721 ctaatatcct tatattcatt cacacttaga ttatcaagtt ttttctttga cattaattca
     9781 atttttttag aaaattcacc tgtatctgtt tgatttgaaa actgctttaa aacattaatc
     9841 ttattttgaa atttctcatt acctacgtcg ataccacttc tcgtatcttc tatacgtcct
     9901 ttttctttat ctatataata actttctgtt tctaaaacag gcgtaataaa gatactaatc
     9961 attagtatta acactaatat ccctagagat ataaacatac tcttagattt agccaatttt
    10021 tttatttccc aatttaccaa tatatttctc ctcctaaatc attttaatta cacatatata
    10081 ttaaaactta aatcttatat aaatataata ctaaccttaa ataaagctta aattatttta
    10141 taatttatag gtgtgtttat tattggagtt acatttatta tttttctggg aaattataca
    10201 ttttcaatta tttttattat ttttgatatt tttattgatt ggaaatttta ttgttgttca
    10261 aattgtagtt attctttaaa tttaaagatg gagtttttta agaatagaac ctactgtcct
    10321 tattatggaa agattattaa atagttgcat ataactttat ttatcttgaa tcttattttt
    10381 aaaaacaatt ataattttag aatattttct attctcaaac aagcatgttg aataatggag
    10441 catcttctag tattatatac ttgtagaata aaactaaatt ggtaaaactg ggaatattta
    10501 accttaagga gttaagttat agaaactagt actaaaaaat tttataaatt atgttaattg
    10561 actaatagaa tgcgactttt tatttttact aaatttttta gggggatgga taatatgttt
    10621 attggagaaa tagacaaatg tacacatata ctaactgctt acatcagtag ttcatatgat
    10681 tattgtaatt ttttagatac acagttagat gattttatat cagaatatgg agaaacggta
    10741 gttgaaacct gcttacatca agtcttatta ttagtaagta gatacaatta aaactaaatt
    10801 tttctagaaa aaccttttta gatttatcta ctctaataaa tatataattt tttgtacttt
    10861 ataagtaact ataacttctg ctaaagtaaa gatgtattgg gagtacatct ttactttccc
    10921 attatagaag ttaaaagcat acaccataaa attaatcatc caatacaaat tatatatttc
    10981 ctatgtctaa cttaatttac aagaatcatt ctatatacat attctgcaat ccactaaaca
    11041 tggctctaaa cctttactat tttatacatt ctctataaat catacaaatc atatataaaa
    11101 tccccctaat atgtttaaac cacctataaa ataacaccaa tatttagaat attcaatatc
    11161 aactgcataa ctggtcaata
//
LOCUS       DAFKMX010000125        11169 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFKMX010000125 DAFKMX010000000
VERSION     DAFKMX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.125"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            293..1324
                     /locus_tag="LZB08_002644"
     CDS             293..1324
                     /locus_tag="LZB08_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBM7316136.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1457..2755
                     /locus_tag="LZB08_002645"
     CDS             1457..2755
                     /locus_tag="LZB08_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBM7316137.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRNLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2869..3114)
                     /locus_tag="LZB08_002646"
     CDS             complement(2869..3114)
                     /locus_tag="LZB08_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBM7316138.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3262..4128
                     /locus_tag="LZB08_002647"
     CDS             3262..4128
                     /locus_tag="LZB08_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBM7316139.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4132..4668)
                     /locus_tag="LZB08_002648"
     CDS             complement(4132..4668)
                     /locus_tag="LZB08_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBM7316140.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4859..5062)
                     /locus_tag="LZB08_002649"
     CDS             complement(4859..5062)
                     /locus_tag="LZB08_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBM7316141.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5353..6117
                     /locus_tag="LZB08_002650"
     CDS             5353..6117
                     /locus_tag="LZB08_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7316142.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6506..7819
                     /gene="prdC"
                     /locus_tag="LZB08_002651"
     CDS             6506..7819
                     /gene="prdC"
                     /locus_tag="LZB08_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBM7316143.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8222..8307
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8889..11072
                     /locus_tag="LZB08_002652"
     CDS             8889..11072
                     /locus_tag="LZB08_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBM7316144.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQIPDGEKLSDGQIPDGEKPS
                     DSQVPDGEKPSDGQIPDGEKPSDGQIPDGEKLSDGQIPDGEKPSDGQIPDGEKPLDEQ
                     TPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKVDEQSEINQ
                     VFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVG
                     ASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSIL
                     YAGSEFDGFGYKQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCD
                     KLIEIAKTEEERAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKIN
                     FEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKS
                     VGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSLLPSSKQNLLSYTIEKKNG
                     QEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPP
                     VPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPPVSDTVEK
                     TPELSRDDTIVKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT         4520 a         1248 c         1935 g         3466 t
ORIGIN      
        1 aaaattgatg cttaatcata gataaatatt aataaaatat acaaggggat tgtctaataa
       61 aaagataatc tcctttttta caaataattc taaattgtga tataaatcta aaaattttat
      121 cttaacattt aaccatttaa tgataataat tgatataata aatataaatt aaatatatgg
      181 tgtacttatt ttatgttttt agcaagaaat atagcattta aaagataaat tgtgcaactt
      241 aaatcaaagt ttaagatgaa ctattaacat agatatatta aaaacttttg taatggagaa
      301 tatatgtgat atgaataata aagttaaggt tgcagtatta gatacaggaa tagacaaaga
      361 acatgattac ttaaaagata atttagttgg aggaattgct tttgaatgta tgcatgatta
      421 tatctttata tcagataaat ttgatgatga agatggtcat ggaactgctt gtgcatcaat
      481 aataaagaaa gagtatgaag atgtagagtt atttgtaata aaaattttga gaaaagatgg
      541 tattacaaat ataaaagttc ttgaagaagc attaagatac cttttagaca ctaacataag
      601 attaattaat ttaagtttat cagtcatagg agtagaaagt gttaagggct tatttgaagt
      661 ttgttatgaa ttgtttagaa aaggaaaaat aatagtatgt tcactcgcta atgattttga
      721 cttaagttat ccagctatgt ttaacaatgt tataggtgta agaggattta ctttagatgg
      781 agatgattca ttttggtaca acaaaaaata tgatgttcaa tgtgttatgg atagcaattc
      841 atatataagt tgtgatataa ataattccta tagattacca ccaaaatgta atagttattt
      901 atcagcaaag cttactggta aaatagctaa gatattatca gaaaaaccag gcataacaat
      961 atctgattta aatgataagc ttgaatcatt agcaactaga aatcattggg atagttgtga
     1021 ttttgatgag tgtagtagaa ttgtaaactt taaattggat ttgtatgaca aagaaaatgc
     1081 tctttttatg aaagttgctg acgttgttag ggattgctta gatattgaag agaataatga
     1141 aaaactcttt aagggtagcc tttttaataa ggagataggc ttggtttatg ataattgttt
     1201 taatctgttg aaaaagctag agcatagatt tgacataaag tttaattata tggacatctc
     1261 aaaatatgac ttagtttcaa tatatacact gactgaatta gtggaaagat ataaaaactt
     1321 ataaataaag atttgtagct aaatttaatg tagaaggtat taaattatat gtattctttg
     1381 tcggtagatt aaaagtaaaa aatatactat aattagatag cataagagaa aatgccaata
     1441 cagggggagt tagataatga aatatgaaga agcattagag tacatatcac aaactaataa
     1501 attcggtata agattaggat tagaaaatat cggaaagtta ttagaattat taggaaatcc
     1561 acaagaaact ttgaatataa tacatgtagc tggaactaat ggaaaaggtt ctgtgtgttc
     1621 ttttgtttct aatatactaa gagagtgtgg atataaagtt ggtttatata catctccata
     1681 tttagaaact tttactgagc gtataagagt aaatggacaa aatataccac aggatgatgt
     1741 tgctagaata attgagttaa taaaagaaaa aatagagatt atggtaaaag aaggctatgc
     1801 atatccaaca gaatttgaag tggttactgc aatggcattt tattactatt cagaacaaaa
     1861 ggtagacttt gtggcattag aagttggttt aggtggcaga tatgatgcta caaatataat
     1921 aacaaaatca cttgtaagtg tgatagtatc tataagtttg gaccatacag gtatacttgg
     1981 agatacaata gaaaaaatag cgtacgaaaa ggctggaata ataaaggaaa atggagtagt
     2041 actagtatat gaccagactg atgaagcaaa ggatgttata aagtcagttt gtaaagagaa
     2101 aaaagctaaa tatatagaag ttgattttga tgatataaac ataaaaaaat cagatataaa
     2161 ttctcaaata tacgattgta ctgtaatgaa agaaacttat agaaaccttg aaataaaatt
     2221 gataggcgaa catcaaataa acaattctat cttggcaatt agtgtaataa aatatctaaa
     2281 agacataaat aaattagcaa atataaatga agaatctata agaaaaggac ttataaatac
     2341 taaatggcca ggaagaatag aaaagataaa agaaaatcca atttttataa tagatggtgc
     2401 acataatgaa gatggagcaa aatctttggc aaaagcactt gataagaatt ttaaaggtag
     2461 aaaattaacc ttattaatag gtatgttaga agataaagat attgatagtg ttttagagat
     2521 attgttgcca cattttaata aggtaataac aacaacacca aataacccaa gggccataaa
     2581 ttcagatata ttaagagaaa aagtattaaa atatgtagat gatgtaacat caaagcatga
     2641 gatagaggat gcagtaaatt atacattaga aacatcaagt gaggatgata ttataataag
     2701 tgcaggttca ttatatatga ttggtacagt gagaacttta gtaaagaaat tataatttta
     2761 taaaataagt atacaaataa aaatataaaa aagagttgtc taaaaaatgg acaactcttt
     2821 ttatataaaa taagttttta ataattttac tgatgtttat ataagtaatt atatagattg
     2881 acgtatagct ttagataatt ggtctgggca agaagtacct tttccaccac agtcaatacc
     2941 atttaattta tcagcaactt caagagcatt ttggccaatt attaagcttc taagtcctga
     3001 taaattacca ttacatcctc ctataaactc agcatcaaca atcacattat tttcatcaac
     3061 ttcaaaaatc atttctctac aacatacacc acttggatta aatgttactt tcattaaaaa
     3121 gacctccatt aaatattatt tcttttttta acattaataa taataccaaa tatatgcgta
     3181 ataatgcaac tataaagcat aatacaagct aatgaaataa tatattattg tagtatatgt
     3241 attgtagaga ggaggggtta tatgaaaatc tatatttcta actacttatc aaagagtatt
     3301 agcatagttg attattctac acttgaacta gaaaaagaaa tagtattaga agataatata
     3361 tatccacacc atttttgcat agaaaaagaa aaaaatttaa tatacatacc tagttcaagt
     3421 aatgggatac tctatgtctt agatttaagt aatgataaaa taattgatac tgtctctata
     3481 ggaggaagtc ttagtcaagt tgtacttagc gataatgaat tgtttgtggc aaatgaagat
     3541 tctaatagta tatatgtact agataaaaac acattaaatc caataggaat tattggagtt
     3601 gataatatgc cacatggatt tgactttgat agagaaagca aaaagttata tgtaccgtgt
     3661 ataaactcga tagtatgtat agatacaatt aataaaaata tacaaaagaa aataaatatg
     3721 gactttagag cttggcatat agaacttgat aaacaaaaga aagaaatgta tatatcaaca
     3781 cttgatggaa agcttataat aatagatgaa gtaagcatgg atataaaaaa ggtattatat
     3841 gaatttttat taccagtaga aataagattt aattatagtg gcaaaaaagt ttatgttgct
     3901 gatttgggtt ataataatgt aagaatttta gattatacaa cgagtagata cattggaaat
     3961 attgaaataa atgggattcc ccaaggatta gaaatatcta aagatgaaaa gttactattt
     4021 gtatcagata cacaagaaaa ttcagtaaaa gtttatgaaa ctgcaaataa caaattaata
     4081 aaagaaatca aggtaggaaa agagcctaca actataattt gtttgtaggc tttaatgtat
     4141 gtctcttata agtaaatcga taacttcggc atacatataa gatgcattat ttttccagtt
     4201 attgcaaata agttttgctt gtttctctga tgaaacattt agatttaaat ctattaagtt
     4261 agactgattt tctatgactc taaggttgac aataaattca ttatcacttt gttttacaaa
     4321 actagacttt acttgagtat ctgctagtag attttctttg ttagaatcta tatacttgtc
     4381 tattttttca gtaacactag taggtattcg attaaagaag tattctagac tttctacgcc
     4441 tctttgagta agagcataat attctctgtc agagtcttta tatgtagtca aaaacttaga
     4501 ttccataagc tgagataaaa gctgttgtaa tgaaaaataa ttcatcatct cagtctctaa
     4561 tactacttga gttatttgag aattagttaa atccatttta attttttcta atatatataa
     4621 aattaaaagt ttgtgatagg ctaattcttc agacgagttt tcaaacataa atatcatcct
     4681 ccgtcaaact atttctatat ataatatatt aaactaattt gtacaaaatt aaaagaacaa
     4741 tttgtattgt attattaatc tttaataatt catataaaaa caattttata aataaatata
     4801 gaaaaacagg gctgctaatt agcagccctg ttgataccca ggtgtcaaaa tttactattt
     4861 attttccagc catttgtctt tcagccattt caactaattt cttagtcata tatccaccaa
     4921 catatccatt ttctctagct gttaagttac ctttgtctat gttttcatag ttagataaac
     4981 ctatttcgtt agctatttct aatttcattt ggtttaaagc agcttttgct tctggaacta
     5041 ctgttctgtt tgaatttgac atagtaaatt tcctcctcaa ataattagtt tatgatgtct
     5101 aacaacactt ttatttatgt gatgttaata ctaatttctc caaaataaaa ataatatata
     5161 cagaaaaata aaggtaaaaa tgtaaaaaat ataaaaaaat taaataaatg agtattttag
     5221 agagtttaat taggtcaata taggttttat ttttttagtt attgtcttat aaattaaata
     5281 ttacagtact tttttgagct gaaaaaaaat aaattttaaa tatagaagaa attatttcaa
     5341 agggggaata gtatgaaaag agaagttaaa aggactaaag ctttgatata tagtgtttta
     5401 tttttttcat tattattagt gttaatattt gtttttgttt atggaataaa atacatatgt
     5461 tctaaatctg acaattcaaa aatgcctata tataaagttg atacaaatga gaaaaaaata
     5521 gctttgagtt ttgatgtagc atggggaact gataatatgg atgatatttt aaaaatttta
     5581 gataaacata atgtaaaagc taccttcttt ttagttggta gttgggtgga tgataatgaa
     5641 gaaattgtaa aagctataga tgaaaaagga catgaaattg gaaatcattc aaatactcat
     5701 gccaatttga aggaaatatc aaaagaagac ataacaaaag agatagaaac tacatcagaa
     5761 aaaatatata atataacagg aaaaaggact aatttattta gacctccttt tggggacgta
     5821 aacaataaag ctatggacat ttgtagtgat ttaggttata aagtaattaa gtgggatgta
     5881 gattctatag attggaagga gcttggacca aatcatgtaa ttgagaaagt aataaaagaa
     5941 tctcaacctg gttcaatagt tttattccat gctaatataa atgatgtaga taactatttg
     6001 gatactataa taagtcgact aaaaaaagat ggatacagtc tagtcaaaat atctgatttg
     6061 ttgtataaag ataattatgt agttgattcc aatggagtgc aaaaaactaa gaattaataa
     6121 gtaataaatg tatattttaa gtaaagttgt gataaattat atagtatatg ttagaaaagt
     6181 gtcgataaaa agacggtgtc tattttttga cactttttat acatgtatgc aatatcaata
     6241 attatagatt aaattgatta aaaaagtgtc taataattga cttttttttg atgggtttaa
     6301 aaatttagaa gaattaatga atgctgtaag acagtatttc caaagggtat aagtttgttt
     6361 aaaagttggc atagaaattg ctttgttaat aagcgatatg ttctatagaa ataaacaata
     6421 tgttaagacc ttataataga aaaatagcat taacattatt tgaacagctt ataaaaaaat
     6481 taatatactt aagggggcgt actagttgaa caaactatat tctattttat taaaacaaca
     6541 cgttggtggt ccagataagc cagtagtaag tgttggagat gtagtaaaaa aaggaactct
     6601 aatcgcagaa ccaactggat taggggcaaa tatttatgcc agtgttagtg gaaaaataag
     6661 tgaaataaat gaccaagcga tagtaattga agctgatgaa gtacaagaag atacttttga
     6721 accattaaaa ggagaaggta tacttgactt aattaaagaa gctggagtag taggaatggg
     6781 tggagcagga ttccctactc atgtaaaatt aaacatagat ttaaatggtg gaacaatatt
     6841 agcaaatgca gctgaatgtg aacctttatt agctcataat ataaaagaaa tagaagaaag
     6901 accagaaata gtttatcaag gtataaaata tgctatggaa gttactaatg ctggaaaagg
     6961 tatgctagca ataaaaagta agcatccaaa agcaatagaa gctttcaaaa aagtaataaa
     7021 gccaggagac aacatagaag ttgcagaatt agttgatatg tacccaatgg gagaagagag
     7081 agcaatagta agagacgttc taggaaaatt acttgagcca actcaattac cttcagaggc
     7141 taatgcagta gtaataaacg tagaaacact aactagaata gtagaggctg ttgagcaaaa
     7201 aagaccagtt atatctaaaa atataactgt agttggtcaa ttaaacagtg gaaaagaatc
     7261 tatagtgttt gaagatgtgc caattggaac aactgtcgga gaattaattg aaagagctgg
     7321 tggaataaaa ggtgaatatg gtgaaataat cctaggagga cctttcactg gtaaagctac
     7381 tactttagat gctccaataa ctaaaactag tggtggtata atagttacta tgccatttgt
     7441 taatgaaaag agaaaaatgg gtcttttagt ttgtgcatgt ggacctaatg aagaaagaat
     7501 gagagatata gcaactaaaa tgggagttac tgatatagta tcagttcaaa aatgtaaaca
     7561 agctcaagaa attaaaggtg cattaaaatg tgaaaatcca ggacactgtc caggacaagc
     7621 tcaaaaatgt attgaattta agaaagctgg agcagaagtt atattaatag gaaactgtac
     7681 tgactgtagt aacacagtaa tgggttctgc accaaaatta aaattaggtg tatatcatat
     7741 tactgaccat gtaatgagaa cagttaatca tccattaata agaagaataa aaggtgacgt
     7801 aagattaaaa ataaaataat agataaatag aagttagaca tctttagaag taaaacttaa
     7861 aagttacttc taaagatgtt ttttaatgta ttttgagggc aatatatatt gtaaaataaa
     7921 tggaaaaaat agacaaaata tgctagatat tgtataatga taacaatagc tattacaatt
     7981 aatattggac aaaaataatg atactcaagt atgtggtttg tattaatatg catattatat
     8041 taagtcaact atccgataga gtaaaaggtt tttgtgatat ttgaagatac tctatagtat
     8101 cttttttatt ataaaaacta atttttgtct tttattagat aagtatctag gatactaatt
     8161 tatattttat tgagttgtta aaaaagtgca cttaaaaagt taaaaaagaa aaattaaaaa
     8221 acctatttat agaaactgtg aagtatatct taaacctggg cacttaaaag atatatggag
     8281 ttagtagtgc aacctgctat aaatataaat aggagtaact tttaattgtc aaaaatatat
     8341 aaaataaata cataaataag taattaatag aagatagata taaatatatt tttgaaatgc
     8401 tccctgcatt cataacgagt gtagagtggc agatatctgc ccaaaactcc tttaattgct
     8461 ggaagttcct aaagctaact aaaccacaac ataatcatga aataaggata agtgtgatag
     8521 agtgtgtatt taataagcac ttgaggttac gaaagtagaa aaaattagtt agatagcata
     8581 aggttaaatc ctaagtgcct tgaaaatgga taatcagcag gtaagcttcg aaaagaagaa
     8641 acttcaacga ctagagagaa atctcgtacc tacaagtgtg gggaaatggg gagtacctaa
     8701 tttatggtta ggtaatgaga tagtctgtgc ttaatagaaa tattaagaag ttcataggag
     8761 aactggttag gagtaacgac cctaattgaa cacactaaag atgtattatt gaataagatt
     8821 catataatat ttaataattt taattatatt aagtgaatac aatatatgtt tttagtatca
     8881 ggagaatttt gaaaacaaaa attaaaaaat caagtataat cagtttagta attgctttta
     8941 gtatggtatt tactgcattt acaccaatag tatcttatgc tgacgaagtg actagcaatg
     9001 atacaatact taatggagaa gaacaatcaa atggtcagac accagatgta gaaaaaccat
     9061 cagacggtca ggtaccagat ggggagaaac catcagacag tcaagtacca gatggggaga
     9121 aaccatcaga tggtcagata ccagatggag agaaactatc agatggtcag ataccagatg
     9181 gggagaaacc atcagacagt caagtaccag atggggagaa accatcagat ggtcagatac
     9241 cagatgggga gaaaccatca gatggtcaga taccagatgg agagaaacta tcagatggtc
     9301 agataccaga tggagagaaa ccatcagatg gtcagatacc agatggggag aaaccattag
     9361 atgagcaaac tccagaagaa gaaaagccat tggaagaaga aatagttata gaggaaatga
     9421 gtttaaaaca agatattgat aaaattcttg atatgacttt atctcaaata aataaaatag
     9481 tatataactt ttgggaagat gaagaagatg taaaggtaga tgaacaatca gaaattaacc
     9541 aagtttttac ttcagaagac tcatttatta gcttatggta tgacaaaaaa gcaaaggtga
     9601 aaaattcttg tttactaaaa gaaatagatg gttcagatag accctatcat gatttaagat
     9661 ttgatgatat tacaaagact ttaacttttg actatgtaat aaagggtctt gtaggagcaa
     9721 gctcaaaaga cgacaaatat gtaatagaag gtgaagatgg agagcaaact gcattttgtt
     9781 ataataatca tttaagacca ccaagtagta atggaaagag tccatattta ccagcagaag
     9841 attttaatgg tcaagaaaac caaaatgaag aagcagttaa atctatacta tatgcaggtt
     9901 ctgaatttga tggatttgga tataagcaac aatttaattt aggcgaagaa gaaaatgaca
     9961 taatgactta ctcagctact caaagtgcaa tttggattat tcttggacaa atagatgaac
    10021 aagaaagact taagcagtat caaggctcta tagacctttg tgataaactt atagaaatag
    10081 caaaaactga agaggaaaga gctgagtatc agaagaggaa agaagtagcg ataaatacaa
    10141 aagcttattt agaagcattg ttaaaagcag gtcgtgaaga gttaaagcca aatgatacag
    10201 gaaaacctag tttgagtaat ggtttaacta agattaactt tgaaaaaaat gaagatggta
    10261 cttatgaaac agaagctgta gccttagtag gttatagtgg tgtagtaaag ttaaaactac
    10321 cagatggagt aactgcttat gatgtagatg gtaatataat aggtacaggc gaagtagaaa
    10381 tttcaacgca acagaaattt aaacttaaga gtgttggaaa accagatgcc aaagctaata
    10441 tttctgctgt atcttatgat tatatttttc caaaagctat tcaatattat aaggcagtgc
    10501 ttgatttagg acaaaaagac cattcatctt tattaccatc tagcaagcaa aatttattaa
    10561 gttatacaat agagaaaaag aatggtcaag aagttaactt taatataggt ttaccaacag
    10621 atgatgatga tgttgtaaat ccaccagtgc caccaataga tgatgatgtt gtaaatccac
    10681 cggtgccacc aacagatgat acaaatggac ataagccaaa accatcacca ccaatagatg
    10741 atacagtaat aaatccacca gtaccaccaa tggatgatac aataataaat ccaccagtac
    10801 caccaacgga ggatgcagtg ttaaatccac cagtgccacc aatggaggat gcagtgctaa
    10861 atccaccagt gccaccaaca gatgatacag tgttaaatcc accagtacca ccagtaagtg
    10921 atacagtgga aaaaactcca gaactatcaa gagatgatac aatagtaaaa tcaccaaaaa
    10981 caggtgatga aactcagcta atgtcatatg tatttattag tgtcattgca atttgtggat
    11041 tagcgtatca atgtaaaata aaaagaaatt aatctagatt attattaagt ttaaaataaa
    11101 aagtctcaaa ttgatttata agtcattttg gggctttttt atgtaaatat aatatgcaaa
    11161 tatagtttg
//
LOCUS       DAFKMX010000126        11136 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFKMX010000126 DAFKMX010000000
VERSION     DAFKMX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.126"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            384..1706
                     /gene="dnaA"
                     /locus_tag="LZB08_002653"
     CDS             384..1706
                     /gene="dnaA"
                     /locus_tag="LZB08_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBM7316145.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1948..3054
                     /locus_tag="LZB08_002654"
     CDS             1948..3054
                     /locus_tag="LZB08_002654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBM7316146.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            3191..3397
                     /gene="yaaA"
                     /locus_tag="LZB08_002655"
     CDS             3191..3397
                     /gene="yaaA"
                     /locus_tag="LZB08_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBM7316147.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3415..4530
                     /gene="recF"
                     /locus_tag="LZB08_002656"
     CDS             3415..4530
                     /gene="recF"
                     /locus_tag="LZB08_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBM7316148.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4531..6432
                     /gene="gyrB"
                     /locus_tag="LZB08_002657"
     CDS             4531..6432
                     /gene="gyrB"
                     /locus_tag="LZB08_002657"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBM7316149.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6452..8878
                     /gene="gyrA"
                     /locus_tag="LZB08_002658"
     CDS             6452..8878
                     /gene="gyrA"
                     /locus_tag="LZB08_002658"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBM7316150.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8947..9243
                     /locus_tag="LZB08_002659"
     CDS             8947..9243
                     /locus_tag="LZB08_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBM7316151.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            9243..9503
                     /locus_tag="LZB08_002660"
     CDS             9243..9503
                     /locus_tag="LZB08_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBM7316152.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9515..9832
                     /locus_tag="LZB08_002661"
     CDS             9515..9832
                     /locus_tag="LZB08_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBM7316153.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9857..10243
                     /locus_tag="LZB08_002662"
     CDS             9857..10243
                     /locus_tag="LZB08_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7316154.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            10243..11016
                     /locus_tag="LZB08_002663"
     CDS             10243..11016
                     /locus_tag="LZB08_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBM7316155.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKIL"
BASE COUNT         4525 a         1150 c         2025 g         3436 t
ORIGIN      
        1 ttagttaata ttgtggataa attagttaca aatcattgaa gtttgacata tattttgata
       61 ttataaatat acttgttgat aatgtgcata aatgttatca acttattaga aattattcac
      121 agcttgtgga tacttttgtg tataactttt aagattactg acaatactct agttataatc
      181 accatttatt gtatgtttat gttatttata actttatcta catattgttt atatcttttc
      241 atattttttt aataatttat actattatta atttatcaac accattatcc ataaatttag
      301 ttattctgta aaatttatat atatttttgc ttttctgtta ataactttgt ggaaaatatt
      361 aattattctg ttcggaggtt tatattatgg atatagtttc tttatgggac aaaaccctac
      421 aattaataaa aggtgactta acttcagtaa gttttaatac cttttttaaa aacatcgtac
      481 cattaaaaat acatcttaat gatttaatct tactggctcc tagtgatttt aataaagata
      541 tcttagagaa tagatattta catttaattg aagatgctat tagtcaactg tcattaaaaa
      601 aatataatat taaatttgta ttgagcgaaa aagaggttgc tgatttaaat tctgatagca
      661 cagatttaaa ttatagagtt ctttacccca atctaaatcc aaaatacaca tttgatacat
      721 tcgttattgg taatagtaat agatttgcac atgctgcatg cgttgcagtt gcagaatctc
      781 cagctaaagc ttataaccca ctttttttgt atggaggagt tggattaggt aagactcacc
      841 taatgcatgc tataggtcat catattgtta gtcaaaaaaa agactcaaaa gtagtttacg
      901 tttcttcaga aaaatttact aatgaactta taaactctat aaaagacgat aaaaacgaag
      961 aatttaggaa taaatataga aacgtagatg tattactaat cgacgatatt caatttatag
     1021 caggaaaaga aagaactcaa gaagagtttt tccatacatt caatactctt catgaagcaa
     1081 acaaacaaat aattatatct agtgatagac cgccaaaaga catacctaca ctagaagata
     1141 ggctaagatc gagatttgaa atgggactta taactgatat tcaagcacct gactttgaga
     1201 caagaattgc tatacttaga aaaaaggctc aactagaacg tatagatgtg ccaaatgagg
     1261 ttatgtcata tatagcaaaa aatattaagt caaacataag ggaactagaa ggtgcattaa
     1321 ctagagtggt tgcttattct tctcttagca atagggtaat atcttttgat cttgctacag
     1381 aagctttaaa agatattata acaacttcta aaaatgaaga aataaatgtt ctcagaataa
     1441 aagaaaaagt atcttctgta ttcaatttaa agatggaaga ttttaattct aaaaaaagaa
     1501 cacgttcaat agcttatcca agacagatag ctatgtattt aacgcgcgag cttactgatt
     1561 tatcccttcc taaaataggt gaagagtttg ggggtagaga ccatacaaca gtaattcatg
     1621 ctcatgacaa agtatctaag gatatagaag aaagtgaaga aattaagact aaaatagaca
     1681 aaattatatc agatttgaag ggataattta tacacaattg tatgtttata tctcttgaat
     1741 aacctgttaa tacttttttt gaacaaaaat tattaacaaa ttatcaagtc taaaataaca
     1801 aagatattaa catcttatac acatgttaat atctttgtat ttataatgtt atacatactt
     1861 attaacatta tcaacaacac ctactactat tactactatg attttaactt atttttatat
     1921 ataaaaagcc agaggaggtt atccacattg aaaataattt gtaatcaaaa aatcctagca
     1981 aatagaattg gaattgctca aaaagctatt aatggaaaaa caacaataga attattaaaa
     2041 ggtatactta tatctacaga agaaggtcaa ttaaaactta ctggatatga tgctgaaata
     2101 ggaatagaaa cttatgtaca agcagaaatc atagaaaaag gtgatgttgt tgtagatgca
     2161 aggctatttg gagatataat tagaaaatta ccagattcct ttgttgagat agaaactgat
     2221 tcagaaaata atatttacat aaattgtgta aattcaagat ttaaaattaa aggttatgca
     2281 gcaaaagaat ttcctaaatt accagaatta aatgaagaag atttatatag catacctcaa
     2341 gaaatcctaa aaaatatgat taaacaaact gtatttgcta tctcacagga ccaaacaaaa
     2401 cctgttttaa tgggagaact tttagaaatt gtagatagaa acttaaattt agtagcaatt
     2461 gatggatata gattagctgt aaaaagttgt tcggttgata gtttaactga aaatataaaa
     2521 gtcataattc ctggaaagac acttatagat gtaaatagtt tgctttctgg agaggataat
     2581 gttaaagttg gatttaacga aaaaaatgct atttttataa ttaatgatac gaaaattatt
     2641 acaagattgc ttgaaggaga ttttattgat tataaaaaat tattgccaag agaacataac
     2701 agcagagtta aattaaatac taaggaactt ttaaatagta tagagagagc gtctttattg
     2761 tctcaatcag aaaaaaataa tcttataaag ttatctataa gagataaagt aatggcaatt
     2821 acttctaata cagaaaaagg aaatgtgtat gaagaagttg aaatagattt agatggagat
     2881 tacctagata tagctttcaa ttctagatat tttatcgaag gtttgaaaaa tatagataat
     2941 gaggaaatat ttatagaatt tactacaaat gtaaatcctt gtataattaa accaacagat
     3001 gatgttaatt atatttactt actgcttcca gtaagaatat catcaaatat atagcagaca
     3061 tgaaggagtt gtcttttgta gaaaattttt ctaatcttag acaactcctt ttataagcat
     3121 atttatggta taatatactt ttagtgtatc aataggaatt tgtttaaaat agtgacaagg
     3181 aggaactatt atgactgaaa taactataga atcagaatat ataaagttgg atcaatttct
     3241 taaattagca gagattgcat caacaggagg tcatgcaaaa tttttaattc aagaaggttt
     3301 agtgacagta aatgatgaaa tagaattaag aagaggaaaa aagataaaat caggagatat
     3361 agttgaaata gaaggaacta aaataaaagt attgtaaaaa aaggagaact ttttgtgaaa
     3421 cttaaaagtt tgcaacttgt taattttaga aattataaaa agttgcatct ggaatttaat
     3481 gggaaagtaa atctacttgt tggtaaaaat ggtcaaggaa agacaaatat agtagaatct
     3541 atttatatgc tttcttttgg aaaatcattt aggactaaca aagataaaga aatggttaga
     3601 tttaatagtg aaaacttata tattggagga agtttttcaa aatataataa atatagtcta
     3661 atagagctaa ttattggaaa agataaaaaa gggataagaa taaataaagt tcctttacaa
     3721 aagatacaag aactacttgg caacttaaat gttgttatat tttctcctga ggatttaagg
     3781 ttggttaaag agggtcctaa agaaagaagg gcatttatag acaaagagat tagtcagatt
     3841 atacctaaat attataaata tcttaccaat tataataaaa cactttctca aaggagtagg
     3901 gtgcttaaaa atatacatgt tgatgaagct ttattagatg tatatgatga tacgttagca
     3961 aagtatggaa gttatattta tattttgcgt agagacttta taaaaaagat agctaatata
     4021 tctgaaaata tgcatatgaa tttgactaat ggagttgaaa gattatcaat tcgatataaa
     4081 aatcaaataa atataactga tgaagatact atagatacag tatataataa atttttagcc
     4141 aagttgtcat cgaatagacc taatgatata gaatctaaga ctaccagata tggtatacac
     4201 aaagatgact taaatatatt cataaatgat ttagatgcaa gactttttgg ttcacaaggt
     4261 caacaaagaa ctgcttcaat atcattaaag ttatcagaga tagaattaat aaaaaatgaa
     4321 gttgaagaat atccagtatt aattttagat gatgttttta gtgagttaga tgaagcaaga
     4381 caaaaacttt tagtaaataa tttaagcaat gttcaaatgt ttataacaag tgctgaagtt
     4441 tcacataaaa aaatatttga tgaaaaaaat gttacaatat ttaatattga aaatggcgat
     4501 gttattagca tagagaatgg aggaaattaa atgaaacaag aatacggtgc aagtcagata
     4561 caagtattag agggattaga agccgttaga aaaagaccag gtatgtatat tggtagtaca
     4621 agccctagag gtttacatca cttagtttat gaagttgtag ataatagtat agatgaggct
     4681 ttacaaggat attgttcaga catatatgta tctattaatg aagatggcag tgttttagta
     4741 aaagataacg gaagaggtat acctgttgaa atacatccta aaactggtaa atcaacttta
     4801 gaaacagtat taactaatct tcatgcagga ggaaagtttg gaggcggagg atataaagta
     4861 tctggaggtc ttcatggagt tggtgtttct gtagttaatg cattgtcaaa atggatggta
     4921 gcagaggttt atttaaacgg aaagatatat aagcagacat atgagaaagg attgccaaca
     4981 tctaagcttg aagtagttgg agaatctcaa gataaaggaa caatgattca atttatgcca
     5041 gatgagacaa tatttgatga aatagagttt aaatatgaaa cattggagta taggcttaga
     5101 gaactttctt tcttgaataa aggaataaaa atagtatttg aagataaaag agaaggacaa
     5161 gaaaaaagaa aagaatttca ctatacaggt ggtttggtag aatatattaa gtatttaaat
     5221 aaatctagaa ctggtataca tgatgacata gtttatatag ataaaaaggt tgatgattgt
     5281 tttgttgaac tagctatgca atatacagat ggttatacag agaacatata ttcttttgca
     5341 aacaatataa atacacatga aggtggttct catttaagtg gatttaaagc agctcttact
     5401 aaaactgtaa atgattatgc taagagaaat aaatttttaa aggaaaatga tgttaaccta
     5461 cttggtgaag acataagaga aggtcttaca gcagtagtat cagttaaatt accagaacct
     5521 caatttgaag gtcaaactaa aacaaagcta ggtaatagct ttatgagagg tatagttgat
     5581 agtgtaacag ttgatgaact ggggtctttc ctagaagaaa acccatcaac agcaagaatt
     5641 atagttgata aagctttaag agcacaaaga gctagagaag ctgcaaaaaa agcaagagaa
     5701 ttaacaagga gaaaaagtgt attagaaagt acatctttac ctggaaaact tgcagattgt
     5761 gcagaaaaag atccatctaa aagtgaaata ttcttagtcg aaggggattc agcgggaggt
     5821 tcagctaaac aaggtagaga tagaaatagt caagctatac ttccattaag agggaaaata
     5881 cttaatgttg agaaatcaag actagatagg atactatctt cagatgaaat aaaaaatatg
     5941 ataacagctt atggttgtgg tattggagaa gattttgata tagataaggc taggtatcat
     6001 aaaattataa ttatgaccga tgctgatgta gacggagccc atataagaac tttattatta
     6061 acattcttct ttagatatat gagacctctt atagatgagg ggtatgttta tgcagcacag
     6121 ccacctttat ataaagtgac aaagcaaaag aaggaacatt atgtttactc agataaagag
     6181 ttaaatatct tattagatga aattggaaga aatggtgttg aacttcaaag gtacaaaggt
     6241 cttggagaga tgaatgcaga gcaattatgg gaaacaacta tgaatcctga gacaagaact
     6301 ttattacaag ttactgtaga agatgctgca attgcagatg aagtattttc aatgcttatg
     6361 ggtgataagg ttgctccaag aaaagaattt atagaagaaa atgcaagatt tgttagaaac
     6421 ttagatatat agagagaggg gattagtata catggaagaa aataacaaaa tactccctat
     6481 tgaaatagcg gaagaaatga aaaaatcgta tattgattat tcaatgagtg ttatagctgg
     6541 acgtgctctt cctgatgtta gagatggttt aaagccagtt catagaagaa tattatattc
     6601 aatgagtgag ttaaatttaa ctccagataa accatatagg aagtcagctc gtattgttgg
     6661 ggacgtttta ggtaagtacc atcctcatgg agatactgct gtttattatg ctatggtaag
     6721 aatggcacaa gatttttcaa ctagagcact tttagtagat ggtcatggta actttggttc
     6781 tgttgatggg gattcaccag ctgctatgcg ttatacagaa gctaaaatga gtaaattatc
     6841 attagaacta ctgagagata ttgaaaagga aactgtagac tttaaaccaa actttgatga
     6901 gtcgttaaaa gagccttcag tattgccagc tagatatcct aatttattag taaatggctc
     6961 aaatggtata gctgttggta tggcaacttc aatacctcca cataatttag cagaagtaat
     7021 tgatgcaact gtatatttga tagataatcc agagtgtagt gtagatgatt taataaaatt
     7081 tgttcaagga ccagatttcc ctacagctgc aattataatg ggaaaagaaa gtatagcaga
     7141 agcatacaga actggaagag gaaaagttaa agttaggtct agagctttta tagaagagct
     7201 accaaaagga aaacagcaaa taatagttac agaaatacct tatcaagtaa ataaggctaa
     7261 actcgttgaa agaatagcag agttagttaa agaaaagaga atagaaggta tatcagacct
     7321 aagagacgaa agtaatagaa atggtatgag aattgttata gaattaaaga gggatgctaa
     7381 tgctaatata gtattaaata atttgtataa acattctcaa atggaagata cttttagtat
     7441 aataatgctt gcacttgtag atggtcagcc aagagtttta aatcttaaac aaatattata
     7501 tcattatatt aaacatcaag aagatgttgt tacaagaaga actaaatttg aactaaataa
     7561 agctgaagca agagcacata ttttagaagg attaaagatt gctttagata atatagatgc
     7621 tgttataagc ttgataagag cttcaaagac tgggcaagaa gctaagctag gtttaataga
     7681 aaaattcaaa ttaactgaaa tccaagcaca agctatatta gatatgagac ttcaaagact
     7741 tacaggttta gaaagagata agatagaagc tgaatatgaa gatttaatca agaagataaa
     7801 tagattaaaa gagattttag ctgatgaaag attactttta aatgtaataa aggatgaaat
     7861 tacaataata aaagaaaatt actctgatga gagaagaaca gaaataagac atgctgaagg
     7921 cgaaatagat atgagagatc ttataagtga tgaagaaata gcaataactc ttactcactt
     7981 tggatatata aaaaggcttc catctgatac ttataagagt caaaaaaggg gtggaagagg
     8041 tatttcagca cttacaacta gagaagaaga ttttgtaagg cacttggtaa ctacaactac
     8101 tcatagtaga ctattattct ttacaaataa aggtagagta tttaaattaa atgcatatga
     8161 aataccagaa ggtaagagac aggctaaagg tactgctata gtgaatttac ttcaattgtc
     8221 agcagatgag aaaattgcta ctctaatacc tattgatggt aatgatgaaa atgaatattt
     8281 attacttgca acgaaaaaag gtattgttaa aaagactaag agagaagagt tcaaaaatat
     8341 aaataaatct ggtcttattg ctataggttt aagagatgat gatgagctta ttggagtaga
     8401 acttacagat ggaaaacaag aagtactttt agtaactaaa gaaggtatgt ctataagatt
     8461 tgatgaaaat gatataagat atatgggtag aacagcaatg ggtgtaaaag gtataacttt
     8521 aagtaaagaa gattttgttg tgtctatgaa cctttgcagt aaaggtacag atgtgttagt
     8581 tgtaagtaag aatggttttg gaaagagaac aaatatagaa gagtatagaa gtcaaataag
     8641 agctggtaaa ggaattaaaa cttataatat atctgaaaaa actggtacaa ttgtaggtgc
     8701 agatatggtc aacgaagatg atgagataat gattataaat tctgatggag ttcttattag
     8761 aataagagtc aatgaaatat cactgtttgg aagagttaca agtggtgtta aattaatgaa
     8821 gacgaatgat gaagtcaatg tagtttcgat tgccaaaata aatattgaag aagaatagga
     8881 taaatatcct attctttttg tataatgtag ttagataaaa atttaaaatt gaaaggagta
     8941 attgttatgt gcaataaaag aagttgtaaa aaaggtggat ttttattatt aggagctatt
     9001 ttagggttta tatttggtat gttttttgca cctaaaaaag gttctgaact aagaaaagaa
     9061 acaaaagaga agtttaatga tgtcaaagaa aatccaaaag aagttctaca tgaaacattt
     9121 aatgatgtta aagagagaat aataaattta gtggatgatg ataataatga agaagatata
     9181 aagatttcag aagaagatat agtaataagt aaaagctttg atgatgaggg agatgttaac
     9241 taatgaatgc atgggggtgg caaattggag cagtgttagt aggaagctct gctcttatag
     9301 tagcaattta tttagctaaa actctaaaca gcataaataa ggttgttgaa aaggcatata
     9361 agattgtaga ttataatgaa agacatatac aagatattgt tgaaaatgca tcatcaatat
     9421 caaagggaat agatgatata gtttatgtaa ttaataaaat attgagtata ggaaatatat
     9481 ttaaaattat taaaaggaaa taggaggttt ttacatgtct atgaatattg attcaaattt
     9541 agattcacaa aataaatttt ggaatgtttg tcttgatgga gaacttgatg tttctacagc
     9601 agataaacta aaggaacatt tacatgcttt aatagaaaaa aatatgttag atgtaaagat
     9661 taatcttaaa gatttagatt atatagactc aacaggctta ggagctatga taggtgtatt
     9721 aaaaaaatta aaaataaatg aaaaagagat atacatagta aatcctaaga gtaacgtaag
     9781 aaagattttt actataacag gtctagataa aatatttaaa gtggagggat aaactttggc
     9841 ttgtgagact ataaagatgg aaattactac aaatccagat tatgttagta taataagatt
     9901 aacagcatct ggaattgcca ataagatggg ttttccaata gatgatatag aagatataaa
     9961 ggtagcagta tctgaagcat gtacaaatgc aataaaacac agtaaagatg atgtgtttaa
    10021 tatagtattt aatattttag ataatgcaat taatatagaa attcaagata atggaaaagg
    10081 atatgatgtt agctcaataa gtacaccaga tttagagaat ccaaaagaga gtggtcttgg
    10141 attgtttata attaaaactt taatggatga tgtagatatt gaatcagaac ataatcaagg
    10201 gacaaaaata aaaatgacta aatatttagg agttgatatt taatgaaaaa tgtagctaat
    10261 gctacacatt acctaaatat ggatacaaaa gagctattta atttatataa taaaaataaa
    10321 aatgtagaca ttagaaatat tcttattgaa agacatctgt atttagcaag attgttagct
    10381 aagaagtata taaacaaagg tgttgatttt gaagatatat atcaagttgc ttcattggca
    10441 ttaatttatg ctattgatag atatgatgtt gagaaaggat ttgagttctc cagctttgca
    10501 acaccaacta tagtaggaga aataaaaaag tattttagag ataaggtatg gacacttaga
    10561 gtcccaagaa gaatacagga attaagcaaa aagataagtg atgcgaagat taaattagaa
    10621 caagaaaata agaagcatcc aaaggtaaaa gatatagctg attatatagg cgttagtgaa
    10681 gaagatgttt tagaggctat ggaggcttct tatggatatc aaccaatgtc tttggattca
    10741 tcaagtaatg atgattcaga ggataaagat ataactctta ttgataagat tggaaaagaa
    10801 gaaggtaact ttggaagtat agagtatgaa gattttatta ataagtttat tgaaacttta
    10861 aatgaattag aagttaaaat ttttaaagat agatttttct ttgataaaac acaatctagt
    10921 atagctaaag aactagggat atcacagatg actgtttcta gactagagaa aaaggtagta
    10981 gagaagctga aaaaagaata tgaaaaaatt ttataaaaaa atataaaaaa agtattgacc
    11041 tactgtttta aagatggtat agtattactt gtccttgaaa aaaggaacaa gaaataaaca
    11101 cgaacattga aaattaaaca gtaggttaat ttatat
//
LOCUS       DAFKMX010000127        11127 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFKMX010000127 DAFKMX010000000
VERSION     DAFKMX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.127"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            305..751
                     /locus_tag="LZB08_002664"
     CDS             305..751
                     /locus_tag="LZB08_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316156.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            1132..2049
                     /gene="pstC"
                     /locus_tag="LZB08_002665"
     CDS             1132..2049
                     /gene="pstC"
                     /locus_tag="LZB08_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBM7316157.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            2051..2875
                     /gene="pstA"
                     /locus_tag="LZB08_002666"
     CDS             2051..2875
                     /gene="pstA"
                     /locus_tag="LZB08_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBM7316158.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2894..3658
                     /gene="pstB"
                     /locus_tag="LZB08_002667"
     CDS             2894..3658
                     /gene="pstB"
                     /locus_tag="LZB08_002667"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBM7316159.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3686..4339
                     /gene="phoU"
                     /locus_tag="LZB08_002668"
     CDS             3686..4339
                     /gene="phoU"
                     /locus_tag="LZB08_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBM7316160.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4497..5366
                     /locus_tag="LZB08_002669"
     CDS             4497..5366
                     /locus_tag="LZB08_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBM7316161.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5453..6889
                     /locus_tag="LZB08_002670"
     CDS             5453..6889
                     /locus_tag="LZB08_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBM7316162.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6937..7764
                     /locus_tag="LZB08_002671"
     CDS             6937..7764
                     /locus_tag="LZB08_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBM7316163.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    8060..8322
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8398..11064
                     /locus_tag="LZB08_002672"
     CDS             8398..11064
                     /locus_tag="LZB08_002672"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBM7316164.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDHNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPEKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4403 a         1191 c         2075 g         3458 t
ORIGIN      
        1 atctaaatct atatattatt tttttacctt taatatatat taaataataa aatagttaaa
       61 taataagatt aagaatttct taataaatcc agaagatgct ttcgattata taatatttta
      121 tatttttata ttattaatta tatatgttgc aattgctgca cctacttttt ctggtgagta
      181 tcagacacaa tcagatagca tccttcgttg tacgaaacat ggacatattt gtctagcgtt
      241 tactaaaata ttagctctat tttctatatt tataataatg tttgttatat gtatttctat
      301 ctatttggca atttctgatt tagcttttgg tactgaatgt ttgaaaacct catttcaaat
      361 gttgttttct gtcattagtc tagtaaatat aaatttacta caagcacaga ttcttatagc
      421 tattggtggt ctaatttcaa tacttgcaat ggttagttta acattatttt tatctgcaaa
      481 atgtaaggat tctcttactt ctatgctgat tgctttttct atttgcttaa ttcctatggg
      541 aataggtgtt agttggattg gatacctatt accgccaggg ggaattggtt ttaagattag
      601 tttattatat caaatgacat catttaattt cttacactta ggaggtatga gtttttggac
      661 accacacatt atactgtttt tctcagtgat agaaatacct atttttatat ttttggctat
      721 tcatacctat tgcaaacatc aagtatcgta acaatttgat ggaaataata gtgatataaa
      781 ctagaggata caataattat atatgtagta taaaagttaa atttagttaa caaatcttaa
      841 tagcagatat aggtaataaa ttatacataa actgtattat tatctatatc tgctatttta
      901 atatagagaa acaacagtat attgtaaagt aaatgtcaaa tatagtttta aaagtaaaat
      961 taatgttaaa aagtaaatat aattttacac ttgcttaact taatcctaat atagatttaa
     1021 cttaaaaaaa gtagaataaa agtataggaa atttgatgaa gacgaattat aaaaaattaa
     1081 attttgaata cagaacgaaa atcgataagt atattttaat gggagggtat tatgatagta
     1141 aaatctagat tagtaggttt agaaaaaaat gacaataatg gcaataagac aaaatacgta
     1201 attgaaaaag tagcaaaaaa tatttttcta cttagtgctt tagtagcagt tgcaagttta
     1261 cttttgataa taggattcgt attttataaa ggattaagac catttatatt tgaaggatat
     1321 tcatttatag actttttaac tggtgcagat tgggttcctt ctgccaataa atttggaata
     1381 tcatctatga tagtagcatc aatatttgca acacttggag cacttgttat aggagtacct
     1441 gttggtatat taacctctgt atttatagca gaagtagcac ctaaaagatt agcaaaaata
     1501 atgtcaccag cagttgaact tttagcaggt ataccttcag tattatatgg tgtatttggt
     1561 cttgcagtga tagttccaac tatacaaagt atatttaatc ttccaaaagg tcaaagtttg
     1621 ttagcaatag taatagtatt agcagtaatg atgttaccaa caataatatc tgtatcagaa
     1681 acagcaataa gagcagtacc aaaagcatac aaagaaggtt cattagcact tggtgcatca
     1741 aaaatagaaa caatattcaa agttgtatta cctgctgcaa aatctggagt attagccgca
     1801 gtagtgttag gagtaggaag agctttaggt gaaacaatgg cagtaatact tgttgcaggc
     1861 aactcaccag ttatgccaac tgcaataaca gatagtgtaa gaccacttac aactaatata
     1921 gcacttgaaa tgggatacgc atttggtaca catcaagaaa tgctatttgc aactggagta
     1981 gtattgttta catttatatt aattttaaac ttagttttaa ataaactttc aaataaggcg
     2041 gtaaattaag atgagaaagt tcaaagagaa tctattgaag tcattagtat atctatcagc
     2101 actatttaca atagtatcat tagtagttat agtgggattt atatttataa agggaattgg
     2161 caacatgaat tttaatttct tatttagcaa ttattcagca tcaggtgatg gtggtatact
     2221 tccaatgata attacaacat tatatacagt agtagtttct atagttgtag caactcctat
     2281 aggaatattg gctgctatat atttacaaga atatgctaaa aaaggaaaag cagttacagc
     2341 tataagattt gcaactgaaa gtttatcagg gataccatct atagtatatg gattatttgg
     2401 aggaattttc tttgtagtta cattaaaaat gggatattcc atagttgcag gttcacttac
     2461 agttgcaata ataatattac cagtaataat tcgtacaaca gaagaagctt taaaaacagt
     2521 tcctcaagct tatagagaat catcattggc attaggagct acaaaatttc aaactttata
     2581 taaggtagta cttccaagtg caataccagg aattttatct ggagtaatct tatcagttgg
     2641 tcgtataatt ggagaatcag ctgcaatatt acttacagca ggtactgtag ccaagatgcc
     2701 aggtggaata tttgatagtg caagaacatt aactgtacac tcatacttac tgactaaaga
     2761 gagtggagat atagcaacag cagccagtat aggaattgtt ttaatagtaa ttgtattagc
     2821 actaaacatg ctagcaagat ttgttgctaa gaaattaaat aaagctaatt attaaattta
     2881 agaggtgtga ataatggaat taatcgataa gataaaaatg agtgtaaaag atttggattt
     2941 attttatggt gataagcaag cattaaagaa aataaatatg gatataaaag agaataaggt
     3001 tactgcttta ataggtccat ctggatgcgg gaaatcaact tttataagaa cgttaaatag
     3061 aatgaatgac ttaatcgaag atgtaacaat taaaggaaac atttctgttg atggagagga
     3121 catatatacc tcagatgatg ttataaatct tcgtactaaa gtaggaatgg tatttcaaaa
     3181 gccaaatcca tttcctatga gtatatatga taatgtggca tatggtcctc gtacacatgg
     3241 actaagagat aagaagcaat tagataaaat tgtagaagaa agcttaaaag gtgcagctat
     3301 ttgggatgag gttaaagaca ggcttaaatc atctgcacta ggactttctg gtggtcaaca
     3361 acagcgtata tgtatagcaa gagcaattgc tatgagacca gaagttattc ttatggatga
     3421 acctacatct gccttagacc caatttcaac attaaaagta gaggaactta tagaggattt
     3481 aaaaaaagac tacacaatag tcatagttac acataatatg caacaagctg ctcgtatatc
     3541 agatgaaaca gcttttttct taaatggaga ggttatagaa tttagcgata caaaaactat
     3601 gtttaccact cctgtagata aaagaacaga agactatata acaggtagat ttggttaaaa
     3661 tatataacta taaaggagtt agacaatggt caataatagt ttagatataa gtattgatac
     3721 tttaaaagaa caaacagtgc ttatgatgga aaaatgtgaa gaagcaatag aaaagtctgt
     3781 gacttgtatg atagagaagg atttagaggg tgcaagaaaa gtagtagaag atgatgaaga
     3841 aataaataaa ttaagagaag aaataagaga taagagtata gagctaatag tattaaagca
     3901 accaatggca agggacttaa gatttattta ttcattaggt aatatatcta tagagctgga
     3961 aagaatagga gattatgcag caaatatagc tagagaaatg ttgaagatag gtggagaggc
     4021 ttatataaaa gagctaatag atattcctaa gatggcaaaa gaatgtaagt ctatgatgtc
     4081 tcagactaga gaagctttaa tcttatttga tgcaagtata gcttatgatg ttggaactaa
     4141 agatgatatt gtagatggat tgtatgaaca agtacaaatt gattgtttaa aaataatgca
     4201 tgaaaactca aataccatag accaaggggt aaaacttcta cttataggaa gataccttga
     4261 aagaattgga gaccatataa caaatgtatg tgagaagata atttttgcaa ttgagggtaa
     4321 aatggttgag ataggataaa aaactgcttt aaagcagttt ttttattgtc taaattaaga
     4381 aagaattgat aaaaaaatat gattaatggg taaatttaaa aaattgtgat atactaggaa
     4441 catattaaat atgaggatgc gtattttata taaggtgctt tttaggaggt ggaactttga
     4501 aacttcaaaa gatgggtatg aggactataa aaacaggaat tagtgtcact ttatgtatga
     4561 ttcttggtga ctatttagta gagaatatgt tttattctct ggtagcatgt ataatttctg
     4621 ttcaagatac agtaaaaggt tctttaaaga cgggaatcaa tagagtggaa ggtactattt
     4681 taggtggtat aataggattc atatttgcca tcattaaagc tggagaccca tttttatgtg
     4741 gtctaggagt aatgtgctca atatatttat gcaatctgtt taaaataaag tcagtagtag
     4801 ttgcctgtgt aacctttctg gctatacatc taggtgtagg aaatagtgac cctgtgtatt
     4861 attctttaca tagggtgtta gatacatctg tgggtgtttt gtttggagta cttgttaatt
     4921 attatgttgc tagaccaaat tatctaaata gtgttataga tgagtttaaa aagatagaag
     4981 aactttcaat gttgcttatt gaagataaaa ttcttagaaa agaaaattta aatgtagaga
     5041 aatttgaaga agaagtagaa aaattagaag aaatatattc aaaacttatt gatgagttgg
     5101 attatagttt gagtgaaact aatatagaaa aaattgagaa tgccttaagg atatgtagag
     5161 aaatacattt tcatatgcag tctatagagt tacttgaaaa taaactttat ttgaaagagc
     5221 atacatataa ataccttaag aaaacatata atgtggataa gctagactgg aatatagatg
     5281 aagaaaaaag ccctgtattc aactatcatc taatgaaaat aatgattgaa atagagttgt
     5341 tgagaaagct aaataaatca tattaataat agtagttaaa taataaataa aaaatagtat
     5401 ctttgtaaat attaaacata tataagttta atttatggag gtactatttt ttatgaatac
     5461 tagaaatgac aattctaaaa tactagaaat atataaaatg aagctttttg gcgaacttgt
     5521 aaaagcagtg tggaatgatg atatagagga tattgaaaac ttacctaata aaattttaaa
     5581 tgaagattta aaggataatt taaataatta ttttagtgga aaatttgata aagatactat
     5641 ggtaaattta attagggtaa ctatgggatt ggatgttaca gaagatacag ataaaaattt
     5701 aaaagagtca ctacatgaag ctgtaaattt agatgaagtc aaaagaccaa taatatctgt
     5761 aatatcagat gcttgcaaat attgtgatga aaataataaa gaagatgagt catgtaagat
     5821 aagaagtaag catattaatt gtaatgagga agatgtatgt agtgcttgtg gtgattgtgt
     5881 atcaagatgt aagttgggtg caatttcaga taaaatagaa tttataccaa tgataaaatt
     5941 gttaaaagat ataaagagcc ctgtatatgc aatagttgca ccagcttttg taggacagtt
     6001 tgggaaagat gtaagccctg gtaaattaag aactgcatta aaaaagatag gatttgaaga
     6061 tatgatagaa gttgctttgg cagcagatat gcttactgca aaagaagcat atgattatta
     6121 tgagcatatg aaaaaggacg aagaaggatg ctttataact agttgttgct gtcctatttg
     6181 ggttagtttg atacaaaata gttttcctca aatattggag aatgtatcac catccgtttc
     6241 tcctatgatt gcttgtggga gagctgtaaa aattttaaat ccagatgcta aagttgtatt
     6301 cataggtcct tgtatggcaa aaaagaaaga agcaatgtta gaagatataa aagatgcagt
     6361 tgattttgtg cttacattta aagagttgca agaaatattt gatgcattag agttaaatcc
     6421 agcaataatg gatgagagta atagagtaga ggcgtccctt tcaggtagag tttatgctag
     6481 aacaggaggt gtaagtaaag cagttaagtt atcagttaga agaattgaca aaaatataaa
     6541 atttgaagaa aaggcatttg aaggaacaaa agagtgcatg gacggtctaa agaaagttct
     6601 aaataaagaa atagaagcta catttataga aggaatgggg tgtgtaggag gctgtgtagg
     6661 aggtccgaag cgtattttaa gtgtggaaga aggtactaag aatgttgatg aatatagtga
     6721 aagtacgaat atggaaactc catttgataa tttaaatgta ttacaatttt taacttcttt
     6781 gggaatcaaa agagtagaat ctttaggaga aaaagaagaa gaaaaggtta aaaaaatatt
     6841 tagtagagat ataaaaagta ataagaaaga gaataaatat aaaaaataac tttttaagtc
     6901 ttaaacttat gcttttataa aagggagata tgggggatga aaattaaaga taataaggct
     6961 gtttttcatg tatttataag gatatttatt atagctgttt taattttaac tggaacatta
     7021 ttggaggaaa aaattaataa tgttccagtt gaaaatataa gttcaaaagg tgaaaataca
     7081 ctggaactgc tttctcaaag taatacttta ggagaaatca tatacaaagc atctcaagaa
     7141 gaaacaaaag gtccaggggc tacttctgaa aaagtagcat acataacaat agatgatggt
     7201 ccatcaaaat atacaaatgc actacttgat attttaaaag aaaatgatgt caaagcgaca
     7261 tttttcatgc ttaatcagaa tatgaaaaac cacaaggaag aagtaaggag agtcttggaa
     7321 gaagaaaact caataggttt tcatagtgta agtcatgata tacataagtt atataaaagc
     7381 ccagaagtaa cagttgggga atttaataca tgcaataata ctctacaaga aataactggt
     7441 cagagttcaa aattaataag attaccatac ggaagtaaac catatatgcc agaaggttca
     7501 tataataaac ttatggcaaa tgattattta atttgggact ggaatttgga tacacaggat
     7561 tggaagtctt ctacaagtca gatagtaagt aatatattat attatggaag aaaaagagat
     7621 gaattagtag tattaattca tgagaaagag caaagtttaa atgctttaaa taatattata
     7681 agaatcttaa aagagagagg atatacaata ttgccaatta cagaaaatat aacacctaaa
     7741 aatttctggc aagaaaactt ataataaatt taaatatagt aaaagataaa gtaaattatt
     7801 tataaaataa aacatataat ataaaaaata cgaataagag gtatactgta aggataatat
     7861 aaaattaatg agaaagcatt aaaattaaaa taaaataatc ttgcaattat tgacattata
     7921 agacagaata actgtaaaaa catattttaa aatataacaa ctatataaaa gagaatagac
     7981 ataattaaga aaattcagaa aaaataaaat aaaaataatt gacaaagaga ataatatttg
     8041 tatataataa tatgtaatat taaagactga gaagaagagg agtaagtaaa tactactaaa
     8101 agagaggaaa attcactggc tgtaagattt tctaagtata agatttactg aatgtagctt
     8161 tggagtttct aaactgaact ataagtaagt ttagacggtt agaaccgtta aattctttga
     8221 gagtcaaagt tgataaaata acattaacca ataatttaat tatatgttaa tgtattttaa
     8281 gctttggaat taaggtggta acgcgagctt ttcgtccttt ttaaagagga tgaagagctc
     8341 ttttttattt ctaaagatat ttaaaaaatt tatttataaa atacaggagg caggacaatg
     8401 gaaaatacga atttatcaaa aacttataac ccaaaagatt ttgaggctag gctatataaa
     8461 aaatggatgg atgaagggta ttttaaatct aaaccaaatc cagataaaaa accatttaca
     8521 ataatgatgc caccaccaaa tataacaggg caattgcata tgggacacgc tcttgaccat
     8581 acacttcaag atatattaat cagatggaaa cgtatggatg gatatgaagc tttttggctt
     8641 ccaggaactg accatgcttc tatagcaaca gaagttaaag ttgttgaaag aataaaaaaa
     8701 caagaaggca agacaaagta tgaaataggt agggaagaat ttttaaagag agcctgggaa
     8761 tggaaagatg aatttggagg aaaaatatct aatcagctta aacaactagg tgattcttgt
     8821 gattgggata aagaaagatt tactatggat gaagggtgta atgaagcagt tatagaattt
     8881 tttgtaagcc tatatgaaaa aggtcacata tacagaggaa atagaataat aaactggtgc
     8941 ccagattgca aaactacttt atcagatgca gaagttgaac atgaagaaca cgacggaaac
     9001 ttttatcata taaagtaccc actaaaagat agtgaagact ttttagaaat agctacaact
     9061 agacctgaga caatgatagg agatactggt atagcagtaa atccagaaga tgatagatat
     9121 aaacatttaa taggaaaaac agcaatactt ccattagtag gaagagaact tccaatagtt
     9181 gcagatagtt atgttgattt agagtttgga actggtgcag ttaagatgac tccagcacat
     9241 gaccataatg attttgaagt aggattaaga cataatcttg agcagttaaa tactatgaat
     9301 gaagatggaa ctatgaatga agtttgtgga aaatatgaag gtatggatag atttgagtgt
     9361 agaaaagcta tcgtagcaga cttaaaagaa caaggttacc taattaagat aaaagagcat
     9421 aatcataatg taggaacttg ttatagatgc catacagtag ttgagcctag attatcagag
     9481 caatggtttg ttaagatgga agaacttgca aaaccagcta tagatattct taaaaaagga
     9541 gaattagaat ttgttccaga taaatttgat aaaacttatt tacaatggct agaaaatata
     9601 agagattggt gtatatcaag acaattatgg tggggtcatc aaataccagc ttattattgt
     9661 caagagtgtg gagaaatagt agttgctaga aaaatgccag agaagtgtcc taaatgtgga
     9721 agcactcatt ttaaacaaga tgaagatgcc ttagatactt ggttcagttc tgcattatgg
     9781 cctttctcta ctctaggatg gccaaataag actgaagctt tagattatta ttatccaaca
     9841 agtgtacttg ttacaggata cgatataata ttcttctggg tagttagaat ggcatttgca
     9901 ggtatgttct gtatgaatga aaagccattt gaccatgttt tggttcatgg attagtaaga
     9961 gattctcaag gtagaaaaat gagtaaatct ttaggaaatg gtatcgaccc attggagata
    10021 atcgagcaat atggagctga tgcactaaga tttacattaa caactggaaa ttccccagga
    10081 aatgatatga gattctatat ggaaagagta gaatttgcta gaaactttgc aaataagttg
    10141 tggaatgcat caagatttgt atttatgaat attgatgaag atataattaa aaacatgact
    10201 agagaaagtg ttaaagagga cttaactcta gctgataaat ggataatatc aagagcaaat
    10261 aatattgtaa aagaagcaac taataacatg gataagtttg atttaggaat agcacttcaa
    10321 aagatatacg attttacttg gtcagaatac tgtgactggt atattgaaat ggttaaacct
    10381 agattatatg gagaagatgc aaatgctaaa tctgcagcat tatatacatt aacttatgtt
    10441 cttgaaaaaa tattaaaatt attacatcca tacatgccat ttataacaga ggaaatatat
    10501 actcatcttc ctactgttga aggatgtata atagtatctg aatggccaaa atataatgaa
    10561 gaagataaca tggctgaaga agaagatatg atgaacttat tgatggaagg tataagaagt
    10621 ataagaaatg tgagagcaga aatgaatgta ccaccttcta agaaagctaa attgataata
    10681 atcccaagtg aagaaaaaat agaagctatt gagcttggta aagactattt tataacttta
    10741 gcatctgctt caaatgtaga gatagcaaaa gataaatcta atgttccaga agatgcagtt
    10801 ggagtagtta ttgatggtgt tgaaatattt atacctctta atgaattagt tgattttgaa
    10861 aaagaaattg agagattatc taaagaaaag aaaaagttag aaggcgaaat aaagagagtt
    10921 aatggaaagc ttgcaaatca aggtttctta gcaaaagctc ctgaaagttt aattgaagaa
    10981 gaaaaagcaa aaaaagaaaa atttgaagag atgattaaat ctgttgagga aagattaaca
    11041 aatttagaat ctaaaataaa ataaatataa gaattagtaa aaacatacaa aaagtatata
    11101 ttctatactt tttgtatgtt tttcttt
//
LOCUS       DAFKMX010000128        11011 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFKMX010000128 DAFKMX010000000
VERSION     DAFKMX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.128"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1109
                     /locus_tag="LZB08_002673"
     CDS             <1..1109
                     /locus_tag="LZB08_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7316165.1"
                     /translation="KEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATS
                     IDISNDKVFEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEK
                     DLKIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIF
                     KEKLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYI
                     ETGNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFERE
                     KIATTEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDY
                     EVIFSKIYKRVDSIAKVISICENSSFEKFVNLFN"
     gene            1276..2193
                     /gene="pfkB"
                     /locus_tag="LZB08_002674"
     CDS             1276..2193
                     /gene="pfkB"
                     /locus_tag="LZB08_002674"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBM7316166.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            2230..4149
                     /locus_tag="LZB08_002675"
     CDS             2230..4149
                     /locus_tag="LZB08_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7316167.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            4689..5651
                     /locus_tag="LZB08_002676"
     CDS             4689..5651
                     /locus_tag="LZB08_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7316168.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNFKIGILLFFYFLFSQI
                     IFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGI
                     VMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIM
                     HVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            6031..7557
                     /locus_tag="LZB08_002677"
     CDS             6031..7557
                     /locus_tag="LZB08_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBM7316169.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            7585..8142
                     /locus_tag="LZB08_002678"
     CDS             7585..8142
                     /locus_tag="LZB08_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316170.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            8664..9707
                     /locus_tag="LZB08_002679"
     CDS             8664..9707
                     /locus_tag="LZB08_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316171.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            9818..10960
                     /locus_tag="LZB08_002680"
     CDS             9818..10960
                     /locus_tag="LZB08_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBM7316172.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKSTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         4487 a          989 c         1917 g         3618 t
ORIGIN      
        1 aaaaagaatt tatagagaat tatatagaaa attataggag caaaagaagc tcaattttag
       61 aaaatgaaca aaacaaagaa attataataa attcaataaa aacattttgt aaaaatttaa
      121 aattagctac atcaatagat ataagtaatg ataaggtatt tgaagagttt ttatataagc
      181 atattagtag tttatgtaaa aaaataaagt taggtataaa tcaatcacct ataatggctc
      241 aagatataaa aataaaatat ccatttgctt ttgaactagc taatatagct aaaaagacaa
      301 tagaaaaaga tttgaagata gaaattaatg aagatgaagt tgcaaacata gctctacata
      361 taggaggagc agttgaaaga gcatcttata ataataaaga taaggttttc aaaactataa
      421 tagtttgtac ttcaggcata ggcacatcaa tgttaataaa agctaaatta gagaatatat
      481 ttaaggaaaa attagaaata ctaaagatta ttccttcata cttagttgaa tatataaaag
      541 ttatagatgt tgattttgta atatctactg ttcctattga agtaggagat gtgccagtaa
      601 taaatatatc tccaatttta agtgagaaag aaattagatt aattgaaaaa tatatagaaa
      661 caggaaatat atacctagat ttagatataa aaagcctgtt tgatagtgaa ttgtttttta
      721 ctgatttggt gtttaaaagg aaagaagaag ttatagattt tatggcaagt aaacttgtac
      781 aaaagggcta tatagatgag gatatgaaaa aatcttactt tgagagagaa aaaatagcaa
      841 caacagaaat agggaatatg gtaactatac cacatggagc tattggaaaa atttttgata
      901 ataaaatagc aattggtata ttaaagaagc ctatttcttg ggaaatatca gatgttcgtt
      961 taataataat gttagccctt gataaagata aaatattaga ttatgaagta atattttcaa
     1021 aaatatataa aagagtagat tctatagcaa aagttataag tatatgtgaa aatagtagtt
     1081 ttgaaaaatt tgtaaattta tttaattaga tgggtagtat tgtatataca cctgtaaatt
     1141 tggataatat aaaactaaat aagataaaaa cgataaattt ctcttttaat taagagaaat
     1201 tttttgactt tcaaggatat taatataaag ttaagatata aacataaagc aaataaataa
     1261 aaaagaggtg gcaatatgat ttatacagtg acattaaatc catcaataga ttatatagtt
     1321 aagctagatg agctaaagac aggaagtaca aatcgtgtaa aagaagaata cgtttatcca
     1381 ggaggaaaag gtataaatgt atctcgcata cttaaggaac ttggaaatga taatacatct
     1441 ttaggtttta taagtggttt tactggagaa tatataataa gaacattgga agaaaaagaa
     1501 ttgaagacag attttataaa gataaaaagt ggtttttcaa gaataaatgt aaaaataaag
     1561 gcgttaaagg aaactgagat taatggacaa ggtccaaata tagatgatga agacattgat
     1621 actttatata aaaaacttga taagctaaat caagatgata ttttaatatt ggcaggaagt
     1681 ataccatcta ctttagatga aaaactatac gaaaatataa tggctagatt agaaaaaaag
     1741 aatataaaag ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt aaaatacaaa
     1801 ccttttctaa ttaagcctaa taacgacgaa ttagaagaac tatttggtgt taaattaaat
     1861 tcaattgaag acatggtaaa atatgctaga aggttaaagg aaatgggagc tataaatgtc
     1921 ttggtatcaa tgggaaaaga tggagcattg ttgattacag aggaagatga agtgcttata
     1981 agtgatgttc caaaaggaaa agtaaaaaat tctgtaggag caggtgattc aatggtagca
     2041 ggatttatat ctgggtattt gaatactggg aaatatgatt atgctcttaa attgggagca
     2101 gcttcaggta gtgcaactgc tttttcatat gacttagcca aaagagaata cattgataaa
     2161 ttggtaaatg aaatatctgt aaagaagttt taaaatatta aaaaatatat agaaaggtaa
     2221 ttaatattta tgaaaattat agatttatta gatgaaaagt caattaagtt aaatttaaat
     2281 tcaaaaacca aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa tagtggaaat
     2341 ttaaatgata aagaaaacta caaaaaagca attttggctc gtgaagaaat gagtacaaca
     2401 ggaattggag aaggtgtagc tatacctcat gctaaaaatt cttcagttac taaagcttgt
     2461 atagcagcag cagtgtcaaa agaaggtata gattatgaat cgtttgacgg aagtttatca
     2521 aatctatttt ttatgatagc tgcaccagac ggagctaaca atactcatct tgaagtatta
     2581 tcaagattat ctacaatact tatggatgaa gactttagaa ataaacttat aaattcaagc
     2641 tctgaaaaag aatttaagga aataatagat aaaaaggaaa gagaaaaatt cagtgaagaa
     2701 tatcaagatg agaaagtggc tgaaaaaaat ataatagata ctaaagagaa tgatgctaat
     2761 aagtatccta aggttttagc agtaacagca tgtccaactg gaatagcaca tacatttatg
     2821 gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg tatcaataaa agtagaaact
     2881 aatggttcag caggtgttaa aaataagctt acaaaagatg aaatagaaaa tgcaacatgt
     2941 ataatagtgg cagcagataa aaatgttgag atggctagat ttaatggcaa aaaagtcata
     3001 aaaactaagg tggctgatgg tatacacaaa gcggaagaac ttatagacaa ggctgtaaat
     3061 ggagatgctc caatttacca tggaggagat ggaagtcaca acgaatcaaa tgaagaaagt
     3121 gaaagtggat ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa tatgttacca
     3181 tttgttattg gtggagggat acttatagcg atagcctttt tacttgatga ttatactatt
     3241 aacccttcaa actttggttc aaatacacct atagcagcat tttttaaagg aataggagat
     3301 aaagcttttg gatttatgtt acctgtactt gctggatata tagcatactc tatatctgat
     3361 agaccagcgt ttgttgtagg atttgtagga ggtgctctag caggtgatgg tggttcaggc
     3421 tttttaggtg cactacttgc aggttttata gcaggttatt tagtagaagg gcttaagaaa
     3481 atatttagtg tactaccagc ttcattggaa ggaataaaac cagtattgtt atatccttta
     3541 cttggaacac ttttgatggg aattataatg acatttttag taatacctcc agttacagca
     3601 attaataatg caatggttgg atttttaaat ggtttaggtg gaacttcaaa aatagtttta
     3661 ggattagtat taggtggaat gatggctgtg gatatgggtg gacctgtaaa taaagcagca
     3721 tatgtatttg gagttgcatc acttgaatca gggcagtttg aaataatggc agcagtaatg
     3781 gctggtggta tggttccgcc acttgctata gctcttgcaa ctacattttt taagaataga
     3841 tttactaaag aagaaagaga ttctggtaaa gttaattatg taatgggatt atcatttgtt
     3901 actgaaggag ctataccttt tgcagctgga gacccacttc atgttatacc agcttgtgtt
     3961 ggaggttcag cagtggcagg agcacttagt atgttgttca acgcagctct tcgtgcacct
     4021 catggaggag tatttgttat accagtggtt acccatccat ttgcatatat attagctata
     4081 gctgtaggtg ctttggttgg aatgatgctt ttagcattac ttaaaaaacc attaaatcaa
     4141 gaagtatagc aattaaagga tattcaagaa cttaaaatat gatgttttat aaaaattaat
     4201 atattgctga attattttaa aataaggatt ttatgaggct gttatataac gatttgtaat
     4261 tgttatcttg acagtctttt atttttatat aagtaaaata agtgtgtttt gctttatttg
     4321 tatttaaatt gtttttaagt tagaatataa agaaaattat atattatttt aatattcact
     4381 cgaactagaa aataatgttt gttgtatttt ttgtatataa aaatatattg taatattaaa
     4441 aaaataatag taaactgatt gcataaaaat gatttttata taaattttaa acagaagaat
     4501 aaatttctaa cgaaattgga tatttcttct gtaaatacat attcaaaaaa agaattataa
     4561 gagtttagtc agcagtcgtg taagtaataa ggactttata aatatataga aatataatga
     4621 tatgagtata agtagaaaaa ttttatgatt tttacaatta ttttttataa aaaaggaggt
     4681 tatgtttgtt gaatttacaa tcaaaatggg taaacttttt aaagccaaca tatgtcagtg
     4741 atgaaataaa aagaaaaaaa ctacaagtta taatattttt ttctataact tatggtttat
     4801 cttattcact aggtttatta gcaatatgtt ttaatcattt aatagattca gaaattttag
     4861 ctcaatttat gatgatattg ccattatctt cagtttctat tgcaaaattt tacacagagg
     4921 gaagaaataa tgataaatat aatttctatt catcagtaat attatttttt attatttact
     4981 tttttatatt tattattgaa tcattaaagt taataagtat taatcaattt caattaatgt
     5041 atcttatgtt aattttaata agtagtttat gtgttattgt ttattcttat aaaataacaa
     5101 gtttatatat ttttaaaaat tttaagatag gtattttgct atttttttac tttttgtttt
     5161 ctcaaattat ttttggatta ataataagtg ataatcaatt tgattataag aatttcttga
     5221 attatattct tcttcctata atatcattag tatacattta tcctttttta tgtgaagagt
     5281 atggttggag atcttttttg caaagtattt tatttgatag atttggcaaa aaaataggga
     5341 ttgtaatggt aggcacttgt tggagtttat ggcacttacc tttacaattt acactatata
     5401 gtcctcaaac tccaataatt ggagctatag cccatttgat atatggtatt ggactttcta
     5461 tatttttagg ttatgtttat atgaaaacta agaacatatg gttttgctct ataatgcatg
     5521 ttctaattaa tagcttagga ttaattttta atgattctga aataattatt aattaccata
     5581 gtattataca aagattgata tttatactat tattttatac tccttttcta tttacaaaag
     5641 aatataagta agaattacaa ttaatttata taaaataaat ttttaagtaa atatttttat
     5701 agagagattg ttatattaca tatcagaaaa atattaaact aactaaatca ataaaaaagt
     5761 tggtggataa caatttataa ttgttagcac aacatcatct tatgtattga aatctaaaat
     5821 atcctcattt ttacttagca taaatactat taattaagaa aataatatag aaattataaa
     5881 tatatataag tttagtctgg tgtataaaac aagaaaacta agtgattttt aatttagctt
     5941 tttattaagc ttatttttta tactatttat aataaataaa ctccatgatg ggaaaactat
     6001 tactatttaa tatgtaaagg agtattttaa atgaatgaat catattggtt tttaaactca
     6061 agtccaaaag aatataacaa attaggtgaa aatataaaaa cagattgtct tatagtaggt
     6121 ggtggaataa ctggattgac aactgcatat ttattagcaa aagaaggtaa aaaagtagtg
     6181 cttgttgagg ctgataaaat aggatatgga acttctggaa ggaatactgg taaggtaact
     6241 tgtcagcatg atatatttta ctctaagata gagaaaaaat atggattaga taaagcaaaa
     6301 tcatattata atgcaaataa tgaagcttta aatttagttg aacaaataat agaagaaaac
     6361 aatataaaat gtgattttaa aagggaaact tcttttgtat ttacagaaaa agaggatact
     6421 ataaagaata ttaaagatga atatcgtaca tgtaaaaaaa taggaataaa ttgtgagtat
     6481 catgaaacta tagaaaatat tccattagat ataaaggggg ctattagttt tactaatcaa
     6541 ggtcaattta acccaaagaa gtatattgat ggattggcaa aagcagcagt aaatttaggg
     6601 ctaaaaatat atgaaaatac accagtagta gaccttgaaa aaggaaaaat atgtagggtt
     6661 aaaacaagag aggataatat aatagaagct gagaatgtta taatttcatc tcatagtcca
     6721 tggtatgatg gattgaattt ttattttgca aaagaatatg cagagcgtgc ttatttaatg
     6781 gttgcagttt tggaaaataa attagcagat ggtatgttta taagtataga tgacccatca
     6841 ataactttta gacaatataa tgatggaagt gaaaatttac tgatttttgg aggaggagac
     6901 cataaagttg gtcagggagg aactgagaaa gaaatatttg atgatttaga gcattatggt
     6961 aaagaagtct ttaaggtaaa agattttaag gggaaatggt ctgctcaaga caatatgagt
     7021 tttgacaatg ttccttatat tggttatatc aacaaaagag aagacaatat ttatgtagct
     7081 acagggttct ctaaatgggg aattactaat ggaactgctg ctggaataat aataaaagat
     7141 ttgataataa ataataattc tgattataaa gatactttta atccatccag attaggtagc
     7201 tacttttcaa aagattttat aaaggaaaat gcaaacgtag caataaatta tgtaagtgga
     7261 aaattaaaaa taggtagtgg agatatgcct aaaaataatg gagaaggaaa gattgttaat
     7321 attgatggaa aaagatatgg tgtatataag gatgataatg gagattttta tattgtagac
     7381 actacatgta ctcatttagg atgtgaactt aattttaaca gtgaagaaaa aacatgggac
     7441 tgtccatgtc atggttctag atttgactat aagggaaata tacttgaagg accagctctt
     7501 aagccattaa aactttatgg acatggtgat aatgatgtta atcctaagtt attataaaat
     7561 tatttttagg aaggtaaagt gtatatgaat aaacttttaa agccaataac tatactattg
     7621 tatatagttt atattttatc tgccgtattt tttgtagcaa acaaagaata tggaagtcta
     7681 ggtctggtag caattagttt agttggtact ttcattttga gttttataaa taagcaaacg
     7741 gaaagattat tgagtaaaga attatatata atgcttgctt tatttataat gttttcatca
     7801 ttgttaggta cttgttttaa tttttatgat ataaattatt atgatgattt tttgcattta
     7861 tggtcaggta taatatcttg tattgtggct ttttctataa ttaaatactt ttatacacaa
     7921 agagagataa gtaaaatgag taaagtgttt ttagttatat ttttatttat gtttagtatg
     7981 ggagtagcga gtttatggga aataggtgaa tttttaatgg atacttttgt tgggactact
     8041 acacaggccg gagggttaga agatactatg attgatatgg tagatgcttt aataggcaca
     8101 attataacta ttccttttat agtaaaaaaa tctaatatat agttttaaat tttttatatt
     8161 aattataatg gatttaatta atcatgttga gtttcaaggt ctatatgatt aattaaatcc
     8221 atttttgtta tttataatta ttattgaata tatttttaca tataagatat aaggtgatag
     8281 atagaaattg ttggattatg taaataatta atatataata ccattatggg caataaatac
     8341 aaatcaaatg ataatatagt ctatttatgt aaatgttata ttgtatacta agtataatag
     8401 taagtttttt gatagtggtg tagaaagtaa aaatctacta gcctaaattg atgtatggat
     8461 tagtttagga acagtaattg catatcttaa gtttgatgtc atgttgggct actagaaata
     8521 ggtcatcttt ttcacttgaa tatcttttct ttaaacatgg agagtatcat atcaaaaaca
     8581 tatgaaaatt aagtgattta taagaacagt aatattttat atgatagtat agaatagtaa
     8641 taaagtatta ggaggaacat tgagtgttaa aaaagtggtt tggtattgtg aaaaaaagac
     8701 aaaaaagtga gtcagcaaaa gaagaaggtg aagtaatatt aaaaaacgaa aaaatattat
     8761 ctgaagaaaa gttgatagat gaagaaggag ttgtagttaa tattgataat gaaatattaa
     8821 ctaaagtaga agtagtaaat gatgataatg aaatagaaga aaaaataata gaagaagatt
     8881 ggttaattag tgaaaatacc ataaaattag atgataaaga agcaattatt aatgataaaa
     8941 acatagaatt atgtaaaaaa gaagttcaag ttgaaagtga aaaaataggt ttaaataagt
     9001 ttgaaggact tgaccaaaat gaaaattata atctagaaaa aaatgttatt gaagaaaaaa
     9061 aagtaagtga atgtttgaca gaagaagatt tagagtacat aaaagaaatt aaaataaaaa
     9121 gaggaaaaag tataaaagct ataaacttgt atactaaaga agagtgggtt tttgacactc
     9181 atatacagtg tagtaaaaaa ctcaaagttc cattagggta cataagagaa aatttaaaat
     9241 atggatatat ggattacttt ggagatgcaa taaattattt aagtgaagta ttaaatatag
     9301 atgaatactg caaaagtgaa tggagctatc tcgataatag caaatctcca tctgagatat
     9361 ttaatattct aaacaataaa atatttagta taaggctttc taatgaaaaa agaaatgaaa
     9421 ttttgacaaa tgataaaatt gaagcattaa aaatgaatta tagatttgaa tgtattgatg
     9481 aagaatatga tgaatatttt aaaaaatata agtctataat caaaagaggt ggaaagaaaa
     9541 aagttgaatt agtaaataaa aaaggtgaca ttttagaaat atttaagtct ttagaagaat
     9601 gtgccattta tttgcaaaag gaaaagaatg aagttataca aatgttaaaa tatggagata
     9661 caaaagtagg aagaaacttt ataaggtata gtttgagaag tatttaattt cattttaaat
     9721 tcataagagt ttcatttctg attcacacat ttaatattat tttaatgtaa aataataaag
     9781 gtgaagtatt gcacaattaa aattaaggag ggtattaatg tctgaagaaa aaaatccaaa
     9841 agaaaataca ttagatgaat ctaaaaattc agataatgta aattctaaaa aagatgatgt
     9901 gtcagatgaa aaaagtactg ataacaaagt taaaaaaagg gaaataaagc taaacaacaa
     9961 aacacttaaa gctattgtag caattgttgc agtaggagct atatgtttag gtggaggcta
    10021 tgtttttggc aaagaagctg gaagaaaatt gcctgctact tcaaaacact ataatagcag
    10081 tgaggtagta gctactattg gggataaaaa aataacaggt gaaatgttaa gaaagagaat
    10141 ggagccatta ttttatttaa atgcaaaaac tactatgact gatgacgaaa tacgttcata
    10201 tgaatcaaat atgattgaaa ctatgattac atctgatata ttatcctctg aagcagcaaa
    10261 agagaaaata agtataacta atgaccaact agatgctcag tataataatt taatgagtag
    10321 tatagaatca gcaatgggaa tgactaaaga agagttttta aaacagtttg atttgactga
    10381 aaaatatata aaagatgata tgaaaaaaga gctagtagca tcaaaatatt taaatgaaaa
    10441 atcaaaagtt actgataaag aagcagagaa ctattacaat aaaaacaaga gcaattattt
    10501 acaagttaga gcttcacata ttttaataaa aactgttgat gataaaggta agcaagttag
    10561 tagtagcaaa aaagctgaac ttaagaaaga agctgaagaa atacttaaaa aagcccaagc
    10621 tggggaagat tttgctactc tagctaaaaa atactctgaa gatagtagtg cagaatcagg
    10681 tggagattta ggtttctttg gtaaaggtca gatggtggaa tcttttgaaa aagcagcctt
    10741 tgcattgaag aagggagagg tatctaacaa acttgtagaa agtgattatg gatatcatat
    10801 aattaagaaa actgatgaaa aatatcagcc atttgaagag gtaaaatcag atttagtaag
    10861 cagtttaaca tcagaaaaac aaaacctact tatacaaaat ctaaaagaaa aatataatgt
    10921 taaaattaca gataaatatc aaatgaaagc taaagaatag ataaagtcaa tttattaaaa
    10981 aaaagaaaaa ttaactgtat ggataaaagc t
//
LOCUS       DAFKMX010000129        10918 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFKMX010000129 DAFKMX010000000
VERSION     DAFKMX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.129"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(199..1374)
                     /locus_tag="LZB08_002681"
     CDS             complement(199..1374)
                     /locus_tag="LZB08_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316173.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDMSEV
                     KQEMTVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEI
                     NIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINI
                     MKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQ
                     NKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLESEDKSMKEIQIEQNNILGSLLHN
                     SEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1604..2689)
                     /locus_tag="LZB08_002682"
     CDS             complement(1604..2689)
                     /locus_tag="LZB08_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBM7316174.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2725..4038)
                     /locus_tag="LZB08_002683"
     CDS             complement(2725..4038)
                     /locus_tag="LZB08_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBM7316175.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4039..6330)
                     /locus_tag="LZB08_002684"
     CDS             complement(4039..6330)
                     /locus_tag="LZB08_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7316176.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEQFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6320..7114)
                     /locus_tag="LZB08_002685"
     CDS             complement(6320..7114)
                     /locus_tag="LZB08_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBM7316177.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7153..7599)
                     /locus_tag="LZB08_002686"
     CDS             complement(7153..7599)
                     /locus_tag="LZB08_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBM7316178.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7671..9077)
                     /locus_tag="LZB08_002687"
     CDS             complement(7671..9077)
                     /locus_tag="LZB08_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316179.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSRILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9095..10465)
                     /gene="hydA"
                     /locus_tag="LZB08_002688"
     CDS             complement(9095..10465)
                     /gene="hydA"
                     /locus_tag="LZB08_002688"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBM7316180.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLETKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10598..>10918)
                     /locus_tag="LZB08_002689"
     CDS             complement(10598..>10918)
                     /locus_tag="LZB08_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7316181.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3158 a         2216 c         1134 g         4410 t
ORIGIN      
        1 ttatcatcta aattttaggt tttattttta taaattaatt gtatttacat aaaaaaaaag
       61 acctaaaaac aaatttttaa cggtcttttc tcaatttttt attttcattt ctaaaattta
      121 taataaatag taagaacact ataaattctt ttatataaaa ttcttagttt gtggatgata
      181 aattagtgaa ttaaaatact actttactga ttttagtttg acaatatcac tccaattttt
      241 actagtggct aactctacta aatttaagtc tcttcttatt tcagctatat cctcttttat
      301 agaattagtg tcacctttta tatatgctat gttatgttct atattatcat gagtagcttt
      361 atttatttca gaattatgta ataaacttcc taaaatatta ttctgttcta tctgtatctc
      421 tttcattgat ttatcttcag attctagact atcaatctta atttcaattc catataattt
      481 ttcatttgct atggatacct cgtccttaat tccattcata tcttcattaa tgttatttat
      541 tttattttgc ataaagttta cttctgattt aacttcggaa acatcttctt tcattacagt
      601 tatttcttgt ctcacttctg acatgtcttt tttcattaca tttatctctt gttttacctc
      661 tgatgtatct tctttcatta cggtcatttc ttgtcttact tcggacatat cttctttcat
      721 tatatttatc tcttgtctta cttcggacat atcttctttt aatacattta tctcctgttt
      781 tacttccgac atatcttctt tcattacagt catttcttgt cttacttccg acatatcctc
      841 tttcattgta tttatctctt gtcttacctc ggacatatcc tctttcatta tatttatctc
      901 ttgtcttacc tcggacatat cttctttcat tacaatcatt tcctgtttta cttctgacat
      961 atttcctttt aatacattta tttcctgttt tacttccgac atatcttctt tcattacagt
     1021 tatttcttgt tttacttccg acatatcttc tttcattaca gtcatttctt gttttacttc
     1081 cgacatatct tcttttaata catttatttc ctgttttact tccgacatat cttctttcat
     1141 tacagtcatt tcttgtttta cttccgacat atcttctttc attacagtca tttcttgttt
     1201 tacttccgac atatcttctt tcattacagt catttcttgt tttacttccg acatatcttc
     1261 tttcattaca gtcatttctt gttttacttc cgacatatca ttttttatgc taactacatc
     1321 ttcatttata gtatataata actctaaaat cttctcttgc acttgcatca ccatccctat
     1381 taattattat aacattttac acaaatgcta tacaatgtta acatatatat cagaattatt
     1441 aaaattttaa ttaaacaaaa ttgattcact ttattacttc aaaactaatt aatatctaaa
     1501 ataactacaa ataaacaaaa ataaggtcaa caacaaaaaa ggcttatctc tttgagatag
     1561 cctttcttat ttttatttaa aactttgttt tttataaaat aatctataca ttcagctctg
     1621 aacatgaaat ttttcttcca agtgcttttg gtttacaaac tgatatacaa agtccacaat
     1681 atctacactc ttcttcattc aattccatct tatcattcaa ttttctcgct tgatatgcac
     1741 aaactctctc gcacatctta catttagtac atttttcttc atcataagta aactcaaagc
     1801 taagtctttc attaacatct cccatatgat tatgtactat accacaagca ttttctatag
     1861 tattatatcc taatctatta agattagatt ttaaatcatc atgtatttta gatattactt
     1921 gtgcgccttt taaaattact actgaacata taccaacaaa gtcagcacct gccataatca
     1981 tttctaaggc atcatctcca cttacacatc cacctaagcc aattattggt atattaactt
     2041 gtttttttat ttcatatatt ctctgtaaag ttattggctt gatagtttct ccacttaaat
     2101 atcctattcc atttccacct aataaaggtt gccctgtttc tatgtctatt ctaaatgctg
     2161 gtcctacaga atcacaagca gttattgcat ctgctcctgc ttcttctaat tttttagcaa
     2221 aatcgcctat ctcatctatc ataggaggaa gtttaactat tacaggtata tttactcttt
     2281 cctttgcatc tttaagcatt ggtatcaaat ctctatagtc ataagatact aattcaataa
     2341 agtcagctcc tgcatttgca actttctcta ctaattcgct actttcttca attgtatgac
     2401 ctattgaagc tatacagaca gtcccatctc ttttcatttg tggtatttca aaatctatcc
     2461 attgctctgg ctcaaaatct gtccattttt cattatttaa aagagcatta gctgtatttc
     2521 taaccatatg tggtgctgga tttatagctc tttcttttct tatagtctta gtcacaactg
     2581 cccctgctcc tgcatcactt aatatcttgc atccagctgc actatatgtt aatggtcctg
     2641 aacctattat aagtggtgat tttaattctt tacctaaaaa attatacatt atctgcttcc
     2701 ccttttcata tatatttata ttcgttaaaa tttccaaaat tcacaagcaa cttctttcat
     2761 ttctttaaca atagcttttt tatctaaatc aactaactgt ttttctttta aaatatgtct
     2821 accatctatg tatacatttg atataaattc ttttttaccc tgaactacta tctggtcaaa
     2881 tatattatca agagtcacag gagttttaaa ttcatcttcc ataactatta tatcagcctt
     2941 attaccaact gctattttac cagaattttg aagtcctaat acataagcac catgctctgt
     3001 tgccattcta aatactaaat ttgctggcat tactgatgca tcttcttcaa ctgatttatg
     3061 cattaaaaat gcacctctca taacttcaaa aaaatcattt atatatccat cagtacccag
     3121 tgccactttt atgccttttt taatcatttt tggtactgga gaaaaacctc cacctacttc
     3181 acagttgcta agaggcatat gaaccacttt tacacctttt tcttttaata tatctatttc
     3241 ttcatcattt acttttacac actgagatgc taatacagtt tcatccaata tatctaaatc
     3301 attatagtag tcaactgctt ttttgccaaa atgtttttca gcatagtttg gctcatatat
     3361 actctcacat aaatgaaatt gcattggtgc atttaatttt tttgcatctt ctttagcttt
     3421 cttaataaat ctatcagaac atgtgaagct tgtatgtgta cacattattc catttattaa
     3481 tttactattt tctctactcc actttattaa attagaattt tcatctaaac atctaagtcc
     3541 attttcaaag cttattcttt cacaactttc taaggataaa atcgctttca tgcctatttc
     3601 ttctagtatt tttccttgct caattaaagt tccttcttct gtatttggag cttctagggt
     3661 atcacaaaaa gctaccacac cagattctat aagttctatt gctgatgctt tagttgttgc
     3721 cttcacttct ttcaatccaa ttctattttc tataaatggc caccaatagt catttaagaa
     3781 accttcaaag tcggtaaaat gtacatttgc cggtattccc ctagaaagaa ctccatattg
     3841 atgcatatga ccattgacga atcctggcat tattatagca tctgatttat ctattatttc
     3901 ttcaatattt ttatatttta tttcaacttc ttcatttggc aatatatctt ttataatgtc
     3961 attttctact acaacagcag aatttgtata tatttcatca gcagaagata ttaagtattt
     4021 acttttaata gcaatcattt atttacctcc ctgatttaat tttttcatgg ccatataaac
     4081 tttctcagaa gttataggaa gttctcttat acgaactcct gtggcattgt atattgcatt
     4141 tgctattgct ggtgcaggtg tatgaataac tatttctcct aaagatttag ctccatatgg
     4201 acctgttggc tcataattat ctacaaaatc aacaattatg tttcctatat ctttttttgt
     4261 tggaactttg tattgtaata gattagaagt ttgaagcttt ccatcagaac catgtctagg
     4321 gtcttcatac attgcaagcc ctataccttg agttacacca ccttcaactt gtattctagc
     4381 aagtgcagga ttcataactg taccacaatc tgtagaacaa gcataattta ttactttaaa
     4441 ctcccctgtt gttttatcta actctatttc agcaaatcct gctaagaatg gtttagcact
     4501 ctcttgtata ccataagatt cagatgaata taaaacttct tgatttccac caccaacaga
     4561 ttcttttcct aattcactta acaaaacaaa ctgctctaca tcttcactat aacaaactct
     4621 gtcttcatat agtattaaat catctattgg taaatcgagt ttcttctgtg ctacttttaa
     4681 tatcttttgt tttaaacttt cagcagattt tattgtagca tttccagtta cataagtagt
     4741 acaagaagcg tatgcaccag tatcatatgg acacatatct gtatctcctg tataaacgga
     4801 aattctatca atagatgtat tcaatgcttt tgcagctatt tgtgcaagta ttgtgtcaga
     4861 accagttcct aagtctgaag acccagaaaa caacttataa gttccatctt catccatcct
     4921 catatttact attgccatgt ctatgttggc tataccagaa ccatgagttc ccacagacat
     4981 tccaacagcc ctcaccttat tgttgccaat atccctaaca gggtactttt catcccatcc
     5041 aataagtttt ttacctctaa ttatacattc atcaagtgca caacttctta ttggaaaatt
     5101 cattattccg ccttcagttt gtttttttat aatattcttt aactttattt ctgttgggtc
     5161 catattaagt ttatgcgcta attcatcaat tgcacaatca agtgcaaatg taccttgtgt
     5221 tgctccatat cctcttaatg ctcctcctgg aactaaatta gtatatacag ttctcccatc
     5281 aaattttatt gcaggtacat tattatatgt tggaagtaca ttatgacctg attcagagca
     5341 aactgaaggt ccattatcac cataagcgcc tgtattattc aatgctttca tatctatagc
     5401 ttttatgttt ccttctttat ctgacccaat ttttatatca aaaaacatct gatgtctagt
     5461 attagtcatt gcaaaagttt cttctctagt ataaattagt tttgctggtc tttttgtcat
     5521 ccaagttaca aatgatgcat atatctctgt tacagctatg ttttttccac caaaacctcc
     5581 acctattctt ggttttatta ctctcacttt tgaaagaggt attcccagtg ctttagaaac
     5641 ctgtcttctc atatgataag ctgattggtt tgcagaagtt actactaatc tgccattcgt
     5701 atctatatac gtataagctc tatgagtttc catcatacaa tgggcttgtg attgagtcat
     5761 ataagaatgt tctactatta catcactttc tttaaactct ttttctacat cccctttacc
     5821 cattttttct ctcgatacaa tattttttgt agcatccaat ccaaaatcaa atggacaaaa
     5881 caaatcatct tcttcatgga ttaaaatttt atttccttcc gattctcttg catccaatat
     5941 aggagtcatt acttcatatt caacctttat aagtttcata gctttaatag cagccctttc
     6001 atcttcagcg gctattatag caacttcatc tcctacatat ctaactacgt cttcaagtat
     6061 taattggtca tgaggtgatg cttctggata tgactctcca accattgaat atctataatt
     6121 tggaacatct ttgtaagtga atatgtttac tacacctttt acctttaatg cattttttat
     6181 atctatgttt tttatcttcg caaaagcatg tggacttcta agaagcttaa ttgttaaaac
     6241 atctttatga aaaattaaat cttctgtata tattggtttt ccagtaacaa tggcttcact
     6301 atctattttt cttacctctt tatttaccat cacactcacc tccaatacta ttatacattc
     6361 tcactactaa ggcttttgcc atatcttttc tgtattcttt acttcctctt atattacttc
     6421 caaatgaaag ttcttcactt gatacttcac cagctttttc tatatcattt tctaaactta
     6481 atatttcact tgctttatag gcaatttttg ctcttcttgg tctagcgcct atcgcaattt
     6541 tataaatact tccttctttt aacattgctc catttaatac tgcaaaatca cctgtacatt
     6601 ttcttataga atcaaataca cctatagcat cttttttagg taatataact tctattaata
     6661 tatctttttc aagctcattt tctaaaaaat cagctaagtt cataacccct tttttatata
     6721 acttaacctt tgcatctacc actagtaatg aaggtattaa atctgaaaat ccatacttag
     6781 caaatacgct agctcctact ctagctccac ttctaaattg aactcctact atattagaaa
     6841 cagcgtttga aattacccca ttacagtaat ttttaataac cttatttaat tctaaagttc
     6901 ttaaacttgt atctgctcca attaatatgc tatcatcagt ttcatttata taatctaaag
     6961 ctaagtcttt taaatctatt gctgaaccaa tatttttact acctagtttt aaaaatccac
     7021 atcctccaag tattatgttg ttttttcttg atattagcag ttcataagct tcatctaaac
     7081 tttttggaac tacatattcc tttatagtta tcatactctc tccccatatc aacttaattt
     7141 tcacttaaaa gtctaagtgt ttaaatattt atgtattgct cttaattgac ctttatatcc
     7201 tgtacatcta caaaggttac catttacata atctataact tcttcttctg ttggattttg
     7261 aagctctctt ttcattgcca aaaccatcat tataaatcca ggagcacaaa atccacactg
     7321 gtcagcccct tcgtctgcaa gaaaatctcc aaacttcttt gcttcatcca agacaccctc
     7381 aagtgttgta atatgctgac cttctactct agcagtaaga taattacatg aaagcacagc
     7441 cttgtcattt accatcactg tacataaccc acatgagcct gtatcacacc cttttttaac
     7501 acttacatat ccattttctc ttaatgtatc tgctaaaaaa tcatctggtc ttatacttaa
     7561 ttctttttct ttattattta tggtacattt gataatcaaa taaatcacct caaaactcat
     7621 atttttaagc taaagtttta ctatactttt ttatgaataa attacattta ttaataaagg
     7681 ctattccatt tatgaccttc cattctattt tttatagttc ctcttacaca aagtctttca
     7741 gctgtatctt gagcatcttt tagtgctttc tcttcattag ctgtcaatat tcttgaatct
     7801 tccattatta tatttccatc tactataaca ctctctatat ttttcatact agaagaatat
     7861 actaatgtag ataccggatt atgcataggt atcgcttttg gtgaaagcat tggattaaat
     7921 attaataagt ctgctttttt acctatctcc aatgaaccta tctcatcttc cattccaata
     7981 gctcttgctc catctatagt tgccaactca agaacttttt ctgctgacat tgctaatggg
     8041 tcacacttat taactttatg ttgaagtgct gttaatttca taagttcaag catatcttga
     8101 gagttattac tagcggctcc atctactcct aaactaactg ttattccttt tttaagcatt
     8161 tctggcactg gtgcaacacc tgaagataag tacatattac ttgctgtatt atgagacact
     8221 ttcatatcgt attttttagt aagttccata tctttttcag ttagatacac acaatgtacc
     8281 ataagcacat ttggtccaag tattcctaat ttttctaaaa catctatgtc atattgccca
     8341 tgcaattctt ttgctgcttc tctatcaaaa ggagtctctg atatatgtac tgtaaataaa
     8401 gcatcatcat attcttttac tactctccat agcatttcaa gcatttcttg agaatttgac
     8461 catattgctg caggagctac tcctattttg attcttccat tttctgtatt atggtgtttt
     8521 tcaaacaatc ttcttacatc tttttcaacc gtttctacat cttgcattat tccaggatga
     8581 actccaaatt gagctcctgt attcatacat cctcttccaa gtattcctct tattcctaat
     8641 tctttataag catctattat tccatcacaa agacctggct tactatgagg atacatgtag
     8701 tcaaccattg ttgttatacc acttctaagt ccttcaatac atccaagcat tgcagcatca
     8761 taagtatctt ttggctctaa atagttagct gctggaaatg tcatagtttc taaccagtct
     8821 ttaagtacca tatcatctcc caatcctttt aataaagttt ggaataaatg attatgtgta
     8881 tttataaaac ctgggaataa aactttacct ttagcatcta taattttttt aacatcagta
     8941 tatttacttt ctatctcttt agaatttcca atatcagcaa tcttgttatc ttttactact
     9001 aatgccccat caaaaataac ttctctctct ttattcacag ttactattat ggcattctta
     9061 ataagtatat ccatcattct tatttccccc cgcattattt agtaattttt cttttaatat
     9121 attgacctgt tggatgttct ttaacattac aactttgtac tacatgattt cctctcaaaa
     9181 atacatcctc aactttacat ttaaccttaa atccttcata tggagtataa tctacatttt
     9241 gtttttgagt tttataacta attgtttctt ctttattgat atttaaaatg actatatctg
     9301 catcacttcc tacagctatc tcaccttttt taggatacat tccaaatatt tttgcagcat
     9361 tcgtagaagt aacttccaca aacttattta cacttattct attttctaag accccatatg
     9421 tatataaaag tgctaaccta tgttcaacac caggtgctcc atttggaatc ttactgaaat
     9481 catcaattcc caagtctttt tgccccttaa aattaaatga acagtggtct gtgccaactg
     9541 tttgaatatc tccactagac aatccttccc aaaggtaatt tatgtcttct ttttttctta
     9601 aaggaggaga cattacatac ttagcaccct caaatttatc cttattatat aaattgtctt
     9661 ctaataatag atactgtggg catgtttcta ctatcatatc tatgtttttt tctctagcca
     9721 tttttacagt ttcaagtgac tccctacaac ttaagtggac tacataggat tttgaattag
     9781 ataatttagt tatatcagca agtcttgaaa cagactcttt ctcaactatg ttaggtcttg
     9841 taagtggatg atattttgtc tctaaattac cattttgtat attttcttct atcaatacat
     9901 ctagtaaatc cccattttca caatgaaatc caactataca acctaaatcc cttgctttct
     9961 ttaaaaccct gtatattgtt ccatcatcta ctttcatgcc atcatatgcc atatacatct
    10021 taaaagatac tatcccatcc gaaataagtt tttccatatg attaaatgta tctttattta
    10081 aacatgtaat tgtcatatga aaaccataat cacaataaca atttccactt gcttttttat
    10141 gatatgcttc tacaccttgc agtaggtttt ccccctcatt ggcttctgca aaatctagta
    10201 ttgtagtagt tcctccagct atagctgctc ttgttcctgt ttcaaaatta tctgcagttg
    10261 taatagaacc tgcattcata tcaaaatgag tatgtgtgtc tattccacca ggaattacaa
    10321 ttttacctgt agcatcaata accttatcac catttatttc taagttattt cctatttcaa
    10381 ctatcttttc atcttctatc cttaaatcac ttatataagt cttatcagag gtaattatag
    10441 ttccacctct tagtattgtt cccatatata cctcctaact tttaagcttt aaagtatttt
    10501 ctaagtattg taaaacttta tttaaaattg tattttctaa aggtgctata ttcagcacct
    10561 ctagactata tttaaataat ttattgtgaa ctaaatatta ttttccgtat acagatttta
    10621 atattccata gtaaccttct gttcctataa ataactgttc ttgctctata tactcatcta
    10681 ttgtgtgagc aagattttcc ttagaaggac caaatcctat agtttttatt ccagcttcac
    10741 ctgcatagtg acttccattc gtacagaatg agtattgagt tatttctgaa tctattccag
    10801 cttcttttaa tcccttatac gctgcttgaa caaattcatc ttcttcgtca tataaccaac
    10861 ctgggaagaa tctttcactt tctatagtat ttcctgtata gcaatcagct ttttcaac
//
LOCUS       DAFKMX010000130        10914 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFKMX010000130 DAFKMX010000000
VERSION     DAFKMX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.130"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            188..826
                     /locus_tag="LZB08_002690"
     CDS             188..826
                     /locus_tag="LZB08_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316182.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            833..1675
                     /locus_tag="LZB08_002691"
     CDS             833..1675
                     /locus_tag="LZB08_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBM7316183.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            1736..4363
                     /locus_tag="LZB08_002692"
     CDS             1736..4363
                     /locus_tag="LZB08_002692"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBM7316184.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            4577..4822
                     /locus_tag="LZB08_002693"
     CDS             4577..4822
                     /locus_tag="LZB08_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBM7316185.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(5111..6409)
                     /locus_tag="LZB08_002694"
     CDS             complement(5111..6409)
                     /locus_tag="LZB08_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBM7316186.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(6443..9577)
                     /locus_tag="LZB08_002695"
     CDS             complement(6443..9577)
                     /locus_tag="LZB08_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBM7316187.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            complement(9581..10804)
                     /locus_tag="LZB08_002696"
     CDS             complement(9581..10804)
                     /locus_tag="LZB08_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBM7316188.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
BASE COUNT         3754 a         1620 c         1757 g         3783 t
ORIGIN      
        1 tactaatata gttctattaa ttaaagaaat atcccccatt aatatataat aaatatcttt
       61 aagcgagaga acttagtaaa aaatatatcg tattcaataa aaaatctgtt tacagaatgg
      121 tatatatagt tatataatat actatataag atgcgaaaag ataaaaagta tagcacaatg
      181 aaagaaggtg attattattc agcttaatga aagacaacta aaaattatag atatagttaa
      241 ggaaaatgaa ccaataacga gtgaaagtat tgcctccagc ttgaatgtga ctcgtgctac
      301 acttagatct gatttagcta tattaacaat gacaggaata ttagacgcaa gaccaaaagt
      361 tggatatttt tattcaggag ttagtgaaat aaatttaatt ggtaaaagtg taaaagaaaa
      421 aacagtagag gatataatga gcatgcctgt actagctaag aaggatgaaa gtatatatga
      481 tgtaatagtc actatgtttt tatctgatgt agggagtata gttattatag atgagaatga
      541 agagttatgt ggagttgttt ccagaaaaga cttgctaaaa gcaactatag gaggttctga
      601 tataaataaa atgccaatag gtatgataat gacaagaact ccaaatgtag tcactttaac
      661 taaaggagca agtgttttat tagcttcaag aaaaataata gaacatgaag tagattcgat
      721 tccgattgtt gaatataagg gagaagacaa aaatcatatg agagttgttg ggagaatatc
      781 aaaaaccaac ataactaagc tattcttaga aatagttgac aattaaggag gtatgctggt
      841 gaaaaaccta attatatatg cagtatcaga ttcagtaggg gagactgcac aacaagttgc
      901 aaaggcatgt atgtctcagt tttatgtgaa tgaaacttat gaaataaaaa gatttccata
      961 catgattaat aaaggagtac tacttgaaac tttggagaat gcaaaagctg aaaacgcttt
     1021 aattgtatat acattagttg atgaagaatt atgcagtatt gttgaaagat attgcgaaag
     1081 agaaggtctt agttgtatag atttaatgac agatatacta agagaaataa gcaagagaac
     1141 aggaagaaaa cctaaaagag aagctggtat aattagaaaa ttagatgaat cttactttaa
     1201 gagagttgaa gctatagaat ttgcagtgaa atatgatgat ggaaaagacc ctagaggtgt
     1261 tcttcaagca gacataattt tagtgggaat atcaagaaca tctaaaactc ctttgagtat
     1321 gtatttagct aataaaaata taaaagtagc taatgttcca ttagttccag aaataccaat
     1381 tcctaaagaa gtgtttgaga tagatactaa aaaaataata ggtcttacta attcacctga
     1441 aaagctgaat gagataagaa cccaaagatt aaaagcttta ggattgtcca gtaaagcaaa
     1501 ctacgctaat ttagaaagaa tacttcagga gttagattat tcagaagaga taatgaaaag
     1561 aattggatgc ccagtaataa atgtttcaaa taaggcaata gaagaaacag caggtataat
     1621 tttagacata atgaaagaaa atggtctaaa gatatacaaa gaaatagaaa tttaataatg
     1681 tctattaata aaaatgatat aaattaaaat aaaaagatta atgggggatt taacaatgga
     1741 aactaagtat gtttatagtt ttggtgaagg aagcaaagac atgaaatctt tactaggagg
     1801 taaaggtgcc aatttagcag aaatgactaa gataggctta cctgtccctc ctggattcac
     1861 tataacaaca gaagcgtgta atgattatta tgttaacaat gaaagtataa gagcagagat
     1921 aataaaagaa atagaagagc atttagctac tttagaaaaa gacttaaata aaacattagg
     1981 atgcaataag aatccattat tagtatcagt tcgttctggt gcagtattct ctatgccagg
     2041 aatgatggat acaattctta acctaggtct taatgacaat agtgttgttg gcttagcaga
     2101 ggctacacaa aatgaaagat ttgcatatga tagttacaga agattcatac aaatgttttc
     2161 tgatgttgct atggaagttc caaaatataa atttgaaaac gtattagaca gagtaaaaga
     2221 agcaaaggga tacacagttg atacagaact tacaactgat gatttaaaag aaatagtaaa
     2281 agaatttaaa gcaatttata aaaaagaaat aaaaaatgat ttcccacaag acccaaaaga
     2341 gcaattaatg cttgcaatag aggctgtatt tagatcatgg aataatcctc gtgcaatcgt
     2401 ataccgtaaa ttgaatgata tagctcataa cttaggaaca gcagtaaata ttcaatcaat
     2461 ggtatttggt aatatgggag aaactagtgg tacaggtgtt gcatttacta gaaatccagc
     2521 tacaggagaa aataaattgt ttggtgaatt tttaatgaat gcacaaggtg aagacgttgt
     2581 tgcaggtata agaactcctc aaaatatatc aactcttgca gatgtaatgc cagctgtatt
     2641 cgatgaattt gtaaaaatta ctcatatact tgaaggtcat tataaagata tgcaagatat
     2701 agagtttact atagaaaatg aaagattata tatactacaa actagaaatg gtaagagaac
     2761 agcagcagca gcaataaatg tagcggttga tttagttgaa gctggaataa ttgatgaaaa
     2821 agaagctatc atgagaattg agcctaatca attagaccaa ttattacatc caaaatttga
     2881 agataaggca ttaaaagaag ctaaggttat agcaaaaggt ttaccagcat caccaggtgc
     2941 agcaagtggt aaagtttatt tcaatgctga tgatgtagta aaagcaaatg aaaaaggaga
     3001 aaaagtagtt ttagttagac tagagacatc tccagaagat atagaaggaa tggttaaagc
     3061 agaaggaatc cttacagcta gaggtggaat gacatcacat gcagcagttg ttgctagagg
     3121 tatgggtaaa tgttgtgttg ctggatgtgg agaaataaaa gttgatgagt ttaataaaga
     3181 aataagagca cttgatgatg tagttataaa agaaggtgaa tacatttcta tagatggttc
     3241 tacaggaaat gtgtacctag gagatgttaa gaaaacagaa gtatctttaa ctggaaactt
     3301 tgaaaaatta atgaactggg tagacaaaca caaatgtatg atggttagaa ctaatgcaga
     3361 taatcctaga gatgctcgtg cagctataga gtttggtgca gaaggtatag gtctatgtag
     3421 aactgagcat atgttctttg atgaagctag attgccagca gttagagaaa tgatattatc
     3481 taatacagta gaacaaagag aaaaagctct tgaaaaaata ttaccaatgc aaagagagga
     3541 ttttgtagag ttatttaaag ttatggatgg aaaaccagtt aatataagat tactagaccc
     3601 accactacat gaatttttgc ctcatgatga tgagactata gaagaacttt caaaatctat
     3661 gggaataaaa gtaagtgata ttaagaagag aatagttgat ttagatgagt ttaacccaat
     3721 gcttggacac agaggttgta gacttgcaat aacttatcct gaaatatgtg taatgcaatc
     3781 taaagctata atacaaggtg ctatagaagc aatcaaagca ggtgttaaag tttctccaga
     3841 gataatggtt ccattagttg gagaagtaaa tgagttaaaa ataataagaa aaatgatagt
     3901 agaaactgtt gacgctataa taaaagaaga aggtatagaa gttccttata ctgtaggtac
     3961 tatgatagaa atacctagag cttgtttaac tgctgatgaa atagcacaag aagcagactt
     4021 cttcagtttt ggtactaatg acttaactca aatggcattt ggatattcaa gagatgatgc
     4081 tggtaaattc ttaggtcaat atgtagatga agaaatatta gaaaaagacc cattccaagt
     4141 actagaccaa aatggtgttg gtaagttagt taagatggga gctaaattag gtagagaagt
     4201 taaaccagaa ttaaaactag gaatatgtgg agagcatgga ggagaaccat cttcagtaga
     4261 gttctgttat agtgtaggac ttaactatgt atcttgttca ccatttagag tgccaattgc
     4321 tagacttgca gcagctcaag catctataaa aaatcctaga taataataga ctgcaaaata
     4381 tgtaactaaa taaaaataaa atcccctatt gttcaatgta tcaaattaag tatttgtaaa
     4441 ttgaatatag gggattttta ttataaaaat ttaacttata tagttgtaag tgaaacgtat
     4501 ttaattatat tgcatatatt attgtagaga gaaatttcaa ctatcaatta aattgagttt
     4561 actggagggg ataaagttga caaagtgtcc taaatgtgga aaggaagtat catcacgtcc
     4621 aggtagtata tgcccacgat gtggatttaa agtagcagat gagagtctaa aaataagatg
     4681 tccagaacct agttgtaggg ctttggtatc taggaaactg gattattgtc ctaaatgtgg
     4741 atgcaaactt agaggatata atatttctga atttgttaac atggtgagat gtaaaataga
     4801 tgagacattt gataaaaaat aattaaattt agagttaagc ttttgagggt gtctcaaaat
     4861 gaacttttta gttcataagg cactcttttt gtatggaatc aaatatattt ttataattta
     4921 aacaataaaa aagaggctta tctctatttt gagatagcct ctataaaagt atacagtttt
     4981 ataagtaaat atatttttac taatctattt ctattatttg ccttctgaat attccatgac
     5041 taaagccacg agtgtgcata actatttttt aatcaaacgt atgttaaagt aatctttcta
     5101 tttaccagaa ttattttcca gtattaagta tccaaggttt ttctatacca ttttttagtg
     5161 agttataatt cacaataaga gagtcatagc tatttttagc atcttcatta cttaccactt
     5221 gttttttata atcagttaga gtaattaatc ctaaatcata tcttaattta gtatttttta
     5281 attgtttttc tgataaatca agttgttttt tagtaatctg aattgtatct tctaaagcaa
     5341 gtaattgagc atagctatca attaaccctt ttttaagagc ttttttactg tcttctaatg
     5401 ttactagacc attttcagta ttaaattttt gttgaagata atttgcataa gtagtaagac
     5461 cagcagcata agtctgtctt tcaagattgt atgcttcaac ttctttttta tatctatcat
     5521 aatctttttc atacttatcc ataagaacct tgtattcatc atcagttagg ttaccttgct
     5581 ctggttttgt aggagcaact ggtcttgtaa ggttaggtct atctggtaaa tctgctttgt
     5641 tctcatcttt atcattaaag ctttcttctg tacgttctaa aatcaattgg tcgtatttta
     5701 aatattcatt aaccttacct tccatatatg aatcaactga gccacctact ctaaaagatt
     5761 caaatctata ttctttatcc aattgataat tatttaaatc tatattagtc aataacttaa
     5821 aatagtcttg attgtttttt agttcttgaa gtttagcttt ttgagtattt tgtagtgttt
     5881 gaagttcaag ttcagctgat tcagtttcta aagatgttac taaccctaaa tcttttttta
     5941 atttcatatc tttaatttct ttagttttta tttcaagatt gttttttatc ttatctagct
     6001 cattttctct tgaaactaaa tcgttaaatt tattagtaat atcatttgct atttggtcct
     6061 ccataaagcc tttagcctgt tcactttgat ttttaagaag tccaatttgg tcgtatggaa
     6121 acgagttgtc acttccagta aaactatcaa actcatcttg tacttctaac ttttctttta
     6181 acatttttat ttcttctgat ttcattttta atttttcact atttttaagt gcagcactaa
     6241 cagcttctct agcagtcaaa atttcctttt cagatgttct tttatttgag tcacttaaaa
     6301 tagaattatc aactttgttt atagaagaag tattattatc tgcgtaaact gggattaaat
     6361 tggcagttat tataccaaca ccacagccaa ttgcaactat tttgctcatt tttttattca
     6421 atacaatcac cctccattct atttaaactt tagctttcaa ttttggtttc tttattattt
     6481 tacgttttat acttcctatt ttatctgtta tcttaccaaa tattttaggc attttgtctg
     6541 aaaacttatt ttcccattta tcaaatatta agtatatagt aggtataagc accaaagtta
     6601 taagtgtaga tacagttaga ccacccatta taactatggc tagtggttgc atagtttctc
     6661 caccttcacc aagagatagt gctgttggca acatacctaa aattgttgtt gctgtagtca
     6721 ttaagactgg tcttaatctt gctgcacaac caattgaaac caaaccaatt aaatcattgt
     6781 tacgatttgt ttttctaagt tgctctatgt agtcaataag aactatacca ttattaacaa
     6841 caataccgat aagtaagatt atacctagca taccgacagt acttaaactt tgaccactaa
     6901 ctagaagcga aagcacaact ccaacgaatg caaatggaac acagaacatg attataaatg
     6961 gtttactaaa tgattcaaac tgagctacca ttaccatata aactaatgct atagctatga
     7021 ccattgaaaa tccaagactt gccatagatt cattcatcat ttctgtttca ccaccaacac
     7081 ctatagagta atctttagga attaccattt tattcgtttt ttctagtgct agttttgtag
     7141 cagtaccagc atctaaattg tcaagtgtag cagttatatt tacagaaaaa tctccatcag
     7201 tcctagatat agattgtaga ccatctgcca tttttatatc tgaaaaagca ccaagaggaa
     7261 cttcttttcc agttgcagag tttactttta gttgctttat atcttctaag ctatcaatag
     7321 tatctgcttt aaattttagg tttacatcca ctttgtaatc atcaatagta gcagtggtta
     7381 cggagtcacc atttattgct gtacgtaaca tgcctgcaat ttcagcagta tttacaccat
     7441 attcttgagc cttatgttta tctatagtaa attgagcttc ttgtgttgta tctgtaagag
     7501 aagtttcaac atttctaaat ccttttatag agctaaattc tttttcagct tcttttccca
     7561 gtatttttaa tacatctaaa tcaggacctt tcatttctaa tgtgaagtct gctcctccag
     7621 ctccacccat aacactactg ttttgactta catttatttt acaatctggt acagtagcca
     7681 ctctatcaat tatctctttt tctatttcat cagtagttct ttttctattt ttttgagatt
     7741 ctaattcaat tgctatactt gctttactat tatttgaatc tgatgtcaaa gttgttatta
     7801 aagttttaat ttctggaata tctgagactt ttttctcagt catagataca tagtaatcat
     7861 tggattctag gctaagacct tttggaagtt ctatagaaat agataattca cccttgtctg
     7921 ctgcaggcat aaagtccata cctataaatc ccattccaaa taaggaagct acaaaaataa
     7981 gtaaacttga tattacaact aagcgttgat gtctcaaaca tacacttaat aattttttat
     8041 atgcttcact taccttatca aatataggac tatctttttc ttgcatcttt tgactatttt
     8101 ttcctctgct taattttgca aatacactag gaacgagtgt aattgcacct ataaaggaga
     8161 atgctagtga agctattagt gttttaccta atgccccaaa agttatttta gctatacctt
     8221 ctgtaaacat gataggtaag aatattgcta ttgtggtcaa tgttgatgca agtattgcat
     8281 ttgttacagt tcttgttccc tcaatagcac aatcatcaag actcaatctg ccacttttcc
     8341 tatatttaaa tatattttca attacaactg tagcattatc aacaaccata ccaactgaaa
     8401 ttaccaatga acttgcagta ataagattta aactttcacc agtaaagtag agaattgcta
     8461 tagcacctat tatagaaatt ggtattgcaa ctgcaacaac tagtgaagct cttacacttt
     8521 ttaggaaagc aaatattaca ataaatgcga taacagcact tataagcaag ttgctcatta
     8581 cactgtttat tgattcactt atgtatgaac ttgtatcatt agcaatttta attttaaatt
     8641 gaggattttc cttattcaat tcatctacgt atttgtatgc actctgcata acttttacag
     8701 tgttggcatc ttgttgttta gaaatatcta ttaccaaact gtcttttcca ttgtatctat
     8761 aaatactatc tttgtctgca tatccataat ctacactaca aagattatct aatctaactg
     8821 tatttcctcc ttttacagga atttgaatct gttttatatc atctagggat tctaatttat
     8881 caatcgctct taaaactatt ttatcctcac cttgagtaat tgaaccataa gggaatgttt
     8941 tattactaga ggataagaca ccttttatag tggataaact aactccataa tttgaaagaa
     9001 cagctgggtc agctattaca ttaacctgtg ctttttctcc acctttaagg tctgctgaag
     9061 taacaccatc tatggattct attttaggct gaattacatc ttcagcgtat tttcttaagg
     9121 cttgtgaact attttcattt cctccagata tgacaagttg acctatagcc tgtgcattca
     9181 tatccagctt taaaactgtt ggtttagctg catcatctgg gaggctggtc tgtaatgcat
     9241 caactttaga cctaacatca ttcattattt catccaaatt agttccaaat tcaaactgag
     9301 caactaccat agaaacactt tcttgtgaac ttgatatagt agattttaca tttgaaacgg
     9361 cagataaact ttcatcaact ttttgactaa cttgttcatc aacgtcttga ggaccagcac
     9421 ctgtccaagt agtcatgacc attacaactg gtatttctat atcaggcatt agatttattg
     9481 acattttttt atatccaata ccaccaaaaa ctattaatac taaaaagacc attatgattg
     9541 tgagaggacg tttaacggaa gtctgtgtta aattcatagt ctacttctcc ttttctacag
     9601 gaaatatctt agttccgtca gaaataagac ttatccctcc aataatcact gtatctttat
     9661 cacttacacc acttttaatt tctattttat cctctgttac aattccagtt tgaaccaagt
     9721 gtttctttgc tatattgtct tttgttgcta tgtatacata ttttttacca ttttcttcaa
     9781 atattgcttt tttaggtact gtaacagcac cattttcttt ttcaacactt aagtttactt
     9841 gagcagacat acctgcctta aatttgttgt ttgtattatt tacaagtact ttaacaggat
     9901 ataaagatgt ctgagggtca actactgaag gtacataatc tatttttcct aaaatccttt
     9961 ggtcttcaat tataacatct acatttccat ctttcttaaa tttacctata tctgattctg
    10021 ctacatttaa atcaacttct agtatattag gattagaaat aataaaagct ggttgtgatt
    10081 gtgttatcat ttcctttgaa tcaaagtttt tagctgttat tgtaccatct actggagatg
    10141 ttaatgttaa tttgtctaaa ttactcattg ctaaattata tgatacttga gcttggtcaa
    10201 gctgtttttt agcaagtgct ttagcttgag gtatactttt tgaagttgct gtatcgtatg
    10261 ctctttgagc agaatctaac tgttgttttg tttgctctaa agaaaattct atttttttaa
    10321 attggtctaa actaatgact tcctgttcat aaagaatctt attattgtca taatttcttt
    10381 gagactcctc atattgaatc tttgcattgt caagagctgt ttttgcctga tttacttgtg
    10441 cctctaatga accaccagtg gcacttgaat aacttgcttt tgctaaatct agtgaggctt
    10501 tactttgctt tacactattt tctagctctg gactgcttat actcaataat tcatctcctt
    10561 ttcttacatt ttgcccaagt tttacataca cttcttgaac tgtgccaaca gtctgtgcag
    10621 taacagctgt ttcatcttta acctttgtag ttcctgagaa agaatttgtg ttttcaattt
    10681 ctccacctac tgctttttga acagaaactg ctattggttt ctcaggttca gattcttctg
    10741 tttttccaca tccaactaaa aacagtgaac tacacattaa gaaaactatt aaatatcttt
    10801 tcatatctcc tcctattata tattgcacac tagcttgttg tagaatttaa agtgtcttat
    10861 aatttaagtt tagttttaga ctaattaaaa aacttccctt tattggcaat ttat
//
LOCUS       DAFKMX010000131        10905 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFKMX010000131 DAFKMX010000000
VERSION     DAFKMX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.131"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            266..730
                     /locus_tag="LZB08_002697"
     CDS             266..730
                     /locus_tag="LZB08_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBM7316189.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            737..1231
                     /locus_tag="LZB08_002698"
     CDS             737..1231
                     /locus_tag="LZB08_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBM7316190.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1247..2272
                     /locus_tag="LZB08_002699"
     CDS             1247..2272
                     /locus_tag="LZB08_002699"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBM7316191.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2259..4706
                     /locus_tag="LZB08_002700"
     CDS             2259..4706
                     /locus_tag="LZB08_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBM7316192.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4886..6259
                     /gene="radA"
                     /locus_tag="LZB08_002701"
     CDS             4886..6259
                     /gene="radA"
                     /locus_tag="LZB08_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBM7316193.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6262..7332
                     /gene="disA"
                     /locus_tag="LZB08_002702"
     CDS             6262..7332
                     /gene="disA"
                     /locus_tag="LZB08_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBM7316194.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7421..8515
                     /locus_tag="LZB08_002703"
     CDS             7421..8515
                     /locus_tag="LZB08_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBM7316195.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8644..10821
                     /locus_tag="LZB08_002704"
     CDS             8644..10821
                     /locus_tag="LZB08_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316196.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4565 a         1100 c         2016 g         3224 t
ORIGIN      
        1 caaatgttta gtaataaaga tgagaaggta tatttaaaat ataaagtatt taaagttctg
       61 aaatctaatc atgaagcaaa aaattagcat ttggttatca ataaatataa taaataaaaa
      121 ctcataatta atgaaagaat aaattcatat ctaaatttct ataaaaaatt taaatttata
      181 caatttaggg ctttaaaaat ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa
      241 aataaaaagg tgtgtggtga tattaatggc aactatgaca gatataattg aaaaatttat
      301 taaagatttg atggaagaag ataatagcat acaaattcaa agaaatgaac tagcaaactt
      361 gtttagttgt gcaccatcac aaataaatta tgtcttaaca acaagattta caatagatag
      421 aggatattac attgaaagta aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg
      481 aaaaagtaaa gatgggcata ttagagaact actaaatgaa aaaataggaa gtcagatatc
      541 ctataaaaaa gcaaaggaat tattagaagg tttaaaagag tcggatttga ttaacgaaag
      601 agaattaaaa ttaatattat atgcaataga tgataaatca ttgtgtatgc caatatatga
      661 attaaaagag aaggtaaggt ctaatatttt aaagaatata ataataggat tatttagtat
      721 agaggagtga taatttatgc tatgtcaaaa atgcaataag aataaagcat ctgtatatta
      781 taataagata gtcaatggag aaaaaacaga gatgtattta tgtagtgagt gtgcaaaaga
      841 gaatacagaa atgaatttta acttagatat gcctttttca atgatggata tattttctaa
      901 tttaggtttt caacctaaaa aagaacttga ggaaaagcta gtttgtccta aatgcaatac
      961 aacatatagt gagtttaaga ataatgggag atttggatgt agtgaatgtt ataatgcatt
     1021 tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg catatagaac atacaggaaa
     1081 agcacctaaa aaatcttttt ataagataag tgtagaaaat gagattaaag aactaaaaga
     1141 agatttagat agagctataa aaaatgaaga gtatgaatta gcagctcaat ttagagataa
     1201 aattaaatat ttaaaaggtt ctattgacta agattggagt gatattatga aagagaacat
     1261 agtcatgaaa tcaagagtta ggctagctag aaatttaaat aactatccat ttccaaataa
     1321 attagataaa gaatgtgcca tggaaataat agaaaaagta aaaaatgcgt ttattaattc
     1381 taacttagaa caaaaagaag aatttgattt ctataaaatt gaagatttag accaatctaa
     1441 aaaaatgctt atggttgaag aacatataat aagcccagat ttagcagaaa atgataaaag
     1501 tgcagttata gtaaaaaaag ataaaactat aagtataatg ataaatgaag aagaccatat
     1561 aaggatacaa actatatgtg atgatttaaa cttagaatat gcatatagtg tagcaaatga
     1621 aatagatgat ttgttagaat caagtttaga atatgccttt aacacaaagc taggttatct
     1681 aacatcttgt ccaactaata caggaacagg aatgagagct tctgtaatga tgcatttacc
     1741 agcattaagt caactaggct atatggatga actttataaa atttcatcac aaataggtat
     1801 agcaattaga gggatatatg gagaacgaac agaagcattg ggaaatatat atcaaatatc
     1861 taatcaactt acacttggaa gaacagagtc taatattata gaaaatgtaa gtggacttac
     1921 aaaagatgca atttcaaaag aaataaaagc tagagaaata ttgcaaaaaa aattaggtat
     1981 aaaactagaa gataaaatat ttagatcgat agggacactt gaaaattcaa gagtaatgag
     2041 tagcgcagag gcaatgagcc atctatcaaa tataaaaatg ggaatagaaa tgaactatat
     2101 agataaacta gatttaaggg ctatagaaca acttatgata ggtatacaac cagcgcatca
     2161 gtcaataatg tataagagtg atgatgttga gaatagagat ataaatagag caacatatat
     2221 tagagaaact ttggaaaaat taagaggagg gatgaattgt gaacttcaat agatttacac
     2281 aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc taaaagtcta ggacacaata
     2341 ttgtcggaag tgaacatata cttttaggtc ttttaagaga agaagaaggt atagctgcta
     2401 aagttctaag taaagttgga tttacagaag cttatttaga aggcaaaata gttgatatgg
     2461 aaggtaaggg agaagaaatc tcagaagata tagtattaag tccaagaagt aaacaaatac
     2521 ttgaattatc aggaatgttt gcaaataaat taaaaacaaa ttatattggg actgagcata
     2581 tattattagc aattattcaa gaaggtgaag gaatagctaa taagatttta aattatgctg
     2641 gagtaaatga taggacttta gcccaattaa caattgatat gatgggtatt agtgataaaa
     2701 atcaatacaa agcagaaaat agttacacgg gcaaccagaa tcaagcagaa tctaaagtac
     2761 ttgataagta tggaagaaat cttacactat atgcaaaaca gaataaaata gaccctgtaa
     2821 taggtagaga aaaagaaata caaagagtaa tacaaatatt aagtagaaga actaaaaaca
     2881 atccagtgtt gataggagac ccaggtgtag ggaaaacagc catagctgaa ggtctggcta
     2941 caaatatagc cttaggaaat gttccagaaa cactaaaaaa caaaacttta tactctttag
     3001 agatgggttc attattggct ggggctaagt atagaggaga atttgaagaa agaattaaag
     3061 aagttgtaga tgaagttgtt aaaaatggaa acataatctt atttatagat gagatgcaca
     3121 caataatagg ggctgggtct actggagaag gctctataga tgcttcaaat atattaaagc
     3181 cagccttagc aagaggagaa atacaagtta taggtgcaac tacaattgat gaatatagaa
     3241 agcatgttga aaaagattct gctctagaaa gaagattcca accagttatg gtagatgaac
     3301 caactaaaga ggactcaata aaaatattag aaggattaag agataaatat gaagctcatc
     3361 ataaagttaa aataactgat gatgctataa aggcagctgt agaattatca actagatata
     3421 tatcagacag atatctgcca gataaagcta tagacttgat tgatgaagct gcatcaaaag
     3481 taagattaaa agaaaataca cctccttcag aaataaaaaa attagaacta gaaatagaaa
     3541 atatagataa agaaaaagaa gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa
     3601 taagagatga acaaggtcta cttaaaaaac aattagaaga tgttagagaa agatggaaca
     3661 agtcatctaa acattcagat ttagttgatg gagaagttat tgctgaagta gtgggcttat
     3721 ggacaggaat accagttaat aaaattcttg aagaagaggc tgatagactt ttgagacttg
     3781 aggaaatact gcacaataga gttataggtc aagaacaagc agtaaaatct atttcaaagg
     3841 caatcagaag gtcaagagca ggtcttaaag accctaatag accaattggt tcatttttat
     3901 ttttaggtcc aacaggagta ggtaaaacag aattatctaa ggcattagca gaagtgcaat
     3961 ttggagatga aaatcaaata attagaatag atatgtctga atatatggaa aaacatgctg
     4021 tgtcaagaat gataggttca cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa
     4081 ctgaaaaagt aagaagaaat ccatattcag ttatcttatt tgatgagata gaaaaagctc
     4141 atccagatgt atttaatatt ctgttacaaa ttctagatga tggtagacta actgattcaa
     4201 aaggaagaac tgtagatttt aagaatacta ttgtaataat gacatcaaat gttggagcat
     4261 ctacaattgg tagacaaaaa actttaggat ttagtatagc taaaggagac gaagaagaaa
     4321 aatctcaata tgaaaaaatg aaagaaaata taatgggtga attaaaacaa agattcagac
     4381 cagagttttt aaacagaata gatgatataa ttgttttcca ctcactaaat gaaaatcata
     4441 tatcaaaaat agtactttta atggcagcga aactacaaga gagattaaaa gaaatggata
     4501 taaaattaga aatgagtgat gaggctgtta aattaatttc taaatctgga tttgacttag
     4561 aatatggagc aagacctctt aaaagagctt tacaaaaaga gttggaagat gagctatctg
     4621 aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt agtagcaaaa gtcaaagatg
     4681 aaaaaatagt ttttgaaact aaataattta atagagttgc attttaaaat attgcaaata
     4741 taagttttat aatctaaaca aaattttaag ggcaatgaat ctataataat tcattgtcct
     4801 taattatttt atttaatctt tacatattgt ctttcagata tgctaaaatt atagtataga
     4861 ataaaaaatt aaggatgata aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc
     4921 ttgcggatat gaaacagcta aatggcttgg taaatgtcca gaatgtacaa aatggaatac
     4981 atttgtagaa gaaatagacc aaaaaagcac caaaaaagag gtctttataa tagataaatc
     5041 ttcatctaaa ccagtcagta taaactcaat agaaagtaaa gaagaagagc ggtttacaac
     5101 agatataaat gagttagata gagtgcttgg aggaggtata gttaaaggct ctttagtgct
     5161 tgttggtggt gaccctggta taggtaaatc aacactttta atccaagtat ctagtaatgt
     5221 tgccaattta ggaaaaactg ttttatatat aactggagaa gaatcagaat ctcaaataaa
     5281 aatgagagct aaaagattag gtattaactc agaaaattta tatatatttg ctgaaaataa
     5341 tttaagtata attgaatctt atttagaaag tgtaaatcca gaattaataa ttctagactc
     5401 aattcaaact gtcttcagtc cagaaatatc ctcggcacca ggaactgtca gccaaattaa
     5461 agaaggtact tcaaaattca tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt
     5521 tgggcatgtt acaaaagaag gttcactagc aggaccaaaa ttattagagc atatggttga
     5581 tactgttcta tattttgaag gagaaagata taatacttat agattagtaa gagctgttaa
     5641 aaatcgattt ggttcaacta atgaacttgg agtatttgaa atgagggatt taggattagt
     5701 agaacttgat aatccatcta aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc
     5761 agtaataata tctactgtag aaggtacgag accaatgtta cttgaattgc aagcattagt
     5821 ttctcctaca agttttggta taccaaaaag aacatctaca ggagtcgatt acaatagagt
     5881 aggtatgtta ttagcagtat tagaaaaacg tgttggtttg caaatacaaa atcaagatgt
     5941 ctatataaac atagttggag gaataaagat aaatgaaccc tcaattgatt tagggatagc
     6001 catttctgta gcatctagtt ttagaaatat accgatagat gaagatatag ctgttacagg
     6061 agaagtggga cttacaggag aagttagagc agtaagcttt atagaaaaga gaatagctga
     6121 gtgtaaaaaa cttggattta aaaagatagt agttcctaga agtaattatg atgttgtaaa
     6181 agagaccaaa ggcatagaaa tatggccagt agataactta agacaagcta taaatatagt
     6241 acttgggagg aatcaataaa tatggagaat tttctagata ataaaaatat gctatatgca
     6301 ttaaaaatga tatctcctgg aactccactt agattaggtc taaacaatgt actaagagct
     6361 aagactggtg gattaattgt aattgcaaca aacgaagatg taatgaaaat agtagatgga
     6421 ggatttgcta taaatgcaga atattcacca tcatatctat atgaattagc taaaatggat
     6481 ggagctatag ttttaagtgg tgatgtaaag aaaatattat ttgctaatgc acaacttata
     6541 cctgactatt ttatagaaac atcagagaca ggaacaagac atagaacagc agaaagagta
     6601 gcaaaacaaa ctggtgctat agtcatagga atttcacaaa gaagaaatgt tataacagtt
     6661 tatagaggaa atgagaagta tgtagtcgaa gatatatcta agatatttac taaggcaaat
     6721 caggctatac aaactctgga aaaatataag acagtattgg accaagctgt aacaaattta
     6781 aatgccttag agtttaatga tttggtaact atttatgatg ttgcattagt catgcaaaag
     6841 atggaaatgg taatgagagt tacaagtata attgaaaaat atgtgataga attgggtgat
     6901 gaaggaactt tagtaagtat gcaattagaa gaattaatgg gtacaaccag aatagaccag
     6961 aaattaatat tcaaagatta taataaagaa aacacagaaa taaaagaact tatgaaaaag
     7021 gtcaaaaatt taaattcaga agaactaata gaattggtca atatggcaaa actattaggg
     7081 tatagtggtt tttcagaaag tatggatatg cctataaaaa caagaggtta taggattctt
     7141 agcaaaatac atagactacc aacagcaata atagaaaact tagtaaatta ttttgaaaac
     7201 tttcaacaaa ttttagatgc atctattgaa gaattagatg aggttgaagg aataggtgaa
     7261 ataagagcaa catatataaa aaatggactc ataaaaatga aacaattagt cttattagat
     7321 agacacatat gatgaatttt aaatgaattt tactactttt ttcttgaata taatatacac
     7381 tttggtctat aatagataag tataactagg gggtgaagca ttgataagga aagtaacaag
     7441 aatattgttt attttacttg gatttacaat aggtataaca acctatttaa cattaatgaa
     7501 agattttgaa atattaacat ttggaaaaga aacatatggt tatatcgcag ctgtagtagc
     7561 aggtataata atagccattt taggatatct tatagaacca tgggttgtaa ataaagtaaa
     7621 agaaattgct aaaatagtgg ataaagaatt gtcaaaatat cctcagactg atatattgtt
     7681 agggtctatg ggtcttatag taggatttgt tattgcgtat ttattaagtg gattagtaaa
     7741 tagaatacca atagtaggtg gaatactatc cttactatta tatttattcc taggatatct
     7801 tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt aatattggaa aactaggaag
     7861 attagcaaat cctattaagg ataaagataa ggataaagaa aataaaaaag aagtaaaagc
     7921 cattcctcca aaagtattag atacaagtgt aataattgat ggaagaatag ctgatatttg
     7981 taagactggg tttatagaag gaaaacttat tattcctgct tttgttttgg aggaactaag
     8041 acatattgca gattcttcag atgatttaaa aagagtaaga ggtagaagag gtctggatat
     8101 attaaatata attcagaaag aattaaatat agaagttgaa ataagtgaga gagactttga
     8161 tgatatagca gaagttgata gtaaattatt aaagcttgct caggttttaa atggaaaagt
     8221 tgttacaaat gattataact taaataaagt tgctcaattc caaggtgttg aagtattaaa
     8281 tataaatgag ttagctaatg ctataaaacc agttgctata ccaggtgaag atatggttgt
     8341 tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt gcatatctag atgatggaac
     8401 tatgatagtt gttgatggag gaagaaaata tatgaatgag actataaaag tactcgttac
     8461 ttctgttctt caaactcctg caggtagaat gatatttgca aaacctaaaa attaatatgt
     8521 tcaaagaaac ctataatttg ggctgtttat tttaaataga tggtccaaat ttatttattt
     8581 ataacgataa tgtttaatgt attcttaaag atggggattt aaatcagaaa gcagggagaa
     8641 tttatgagaa aaaagattat atctttctta gcaacattta tcataatatt aacatcagca
     8701 agtcagtata gttttgcaga tgatatatca acacgtggaa aagtaatatt tatagatatg
     8761 aatagaacta gtatgagtaa tatgttaaga ataaaatcat taagagaaga actagacaat
     8821 agagggtata ttggcttgat gaatataaga ggcgataaag gaagtgatga tagaaggtca
     8881 tatgctagta tgggtgctgg tggtagagct aatgtggcta atgaagaaga tattaatttt
     8941 gaaagctcga gtaaggatag aaatatagtt tttgaatcag ctacaggaaa aagtgcaaaa
     9001 ggtataaata atttaactat aaataagtct ataaatgaaa atttaaactt tggagagtat
     9061 ggttctgtat tagggtcatt aggacaaagt ttatctgata atggattaaa agcatctgta
     9121 ttgggtaatt cagatataat tgaaaatggt caacttataa aaaatagaaa cttatgtcta
     9181 actgcaatgg atgaatatgg aagaatacct aatggaaatg tagatactat aaataaaaaa
     9241 gacttatcta tgccttatgg aatatcaact gattatgata aattgattgt agagactaaa
     9301 gaagcttaca aaaataatga tgtgattttt gtggagcttg gagacacata tagattagat
     9361 ttatataaac caaatttaaa tgaaaaaaca tatgagagta tgaaagataa catagaaaaa
     9421 aatatagata tatatttaaa aaatatattt tctatggttg aagaaaatga cactgtctac
     9481 atagcaagtg catttccaag tgatttagat tataaaaata aaagaagact ttcgccaata
     9541 ataaaattaa atggagaagg gaaaggtata ttatcatctt caactactag aagagaaggt
     9601 attgtaacaa atttagatgt tggggttgaa atattagaca actttaatat aaaaaatcaa
     9661 aatatggttg gaagaaaata tgaacttatt aacagagatg ataataaaga atttttgatg
     9721 gatgaatatc aaaagatagt atctatttca agcataaggt ctacaatact gaatggtttt
     9781 gtaagtatag ttttcttgtc atggatagtt gctatgattg caattttatt tagaaagcat
     9841 atatcaaaaa attataaaga aacaacattt tttatattaa aggaactttt aaaaatagga
     9901 atagtaatgc cactgtcatt tatgataaca ccaataatga atttcaaaac accattagca
     9961 atatctttag gaataattat aataacactt actatatatc ttataagtaa agttttaata
    10021 aagaacgatt taaaaaacat gctgtttttt actggattaa ccatagtaat tatggttatt
    10081 gatgctgggt ttggctcata tttaatgaaa agcaatgtta tgagttatga ttgtataatt
    10141 ggagctagat attatggagt aggaaatgag tatcaaggtg tggcaatcgg tagtgcaata
    10201 tttacatttg caattttact tacatataaa aatataccaa agtggtctat aatagttttt
    10261 tctttggtta tcttaattac aagtgcatct ccaattatgg gagcaaatgt tggaagtgct
    10321 atttctgaat gtgttgcatt tttactattt atactattga tatataatgt aaagatagat
    10381 ttcaaaaaaa tcgtattatt aggtatagca gtagtatttg tattaggagt ttttgtagca
    10441 atagatatga ttttgggaag taattcacat ttaggcatgt ttgtaaaaga aatttacttt
    10501 aatgggccag gagaaataat acaaacattt agtagaaaga tagagatgaa tttaaagcta
    10561 gctcaaacca gtgcatgggt aaatatactg ttaactggaa taggtgtaat tttagttctt
    10621 atgattaatc agattagata ctttaagcaa ctgatggatg agtatccaat tgtatttaaa
    10681 ggatttatag catcaatagc aggatgttta gtaactcttt tagtgaatga ctctgggatt
    10741 gtgtcatcag ctacagcctt tatttatgta attgtaccta tgataacatt aagtataaat
    10801 cttacagcac taaaagaata agtactgtat gaataatatg taaatattaa tacattaatt
    10861 taacttcttt atttacaaaa taaagaagaa agtttataaa aaata
//
LOCUS       DAFKMX010000132        10884 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFKMX010000132 DAFKMX010000000
VERSION     DAFKMX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.132"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..1035)
                     /locus_tag="LZB08_002705"
     CDS             complement(<1..1035)
                     /locus_tag="LZB08_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBM7316197.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENDKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFS"
     gene            1102..1473
                     /locus_tag="LZB08_002706"
     CDS             1102..1473
                     /locus_tag="LZB08_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBM7316198.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1510..2184
                     /locus_tag="LZB08_002707"
     CDS             1510..2184
                     /locus_tag="LZB08_002707"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBM7316199.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2433..3272
                     /locus_tag="LZB08_002708"
     CDS             2433..3272
                     /locus_tag="LZB08_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBM7316200.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3516..3612
                     /locus_tag="LZB08_002709"
     tRNA            3516..3612
                     /locus_tag="LZB08_002709"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3550..3552,aa:Sec,seq:tca)
     gene            3763..4470
                     /locus_tag="LZB08_002710"
     CDS             3763..4470
                     /locus_tag="LZB08_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBM7316201.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4612..5448)
                     /locus_tag="LZB08_002711"
     CDS             complement(4612..5448)
                     /locus_tag="LZB08_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7316202.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5636..6541
                     /locus_tag="LZB08_002712"
     CDS             5636..6541
                     /locus_tag="LZB08_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBM7316203.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6550..7734
                     /locus_tag="LZB08_002713"
     CDS             6550..7734
                     /locus_tag="LZB08_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316204.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7849..9150
                     /locus_tag="LZB08_002714"
     CDS             7849..9150
                     /locus_tag="LZB08_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBM7316205.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9104..10147
                     /gene="holA"
                     /locus_tag="LZB08_002715"
     CDS             9104..10147
                     /gene="holA"
                     /locus_tag="LZB08_002715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBM7316206.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10334..10600)
                     /gene="rpsT"
                     /locus_tag="LZB08_002716"
     CDS             complement(10334..10600)
                     /gene="rpsT"
                     /locus_tag="LZB08_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBM7316207.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4262 a         1122 c         1683 g         3817 t
ORIGIN      
        1 agaaaaagat ttatctgtaa gtttaaaatt atcttttata cccttagtta agtatacttt
       61 cttatcctta gttatgaata tagcatctac attatcaatt ttattaatta aattcatacc
      121 tttttcaaga ccaagaccaa atgtagaagt ggataaggca tcacaattaa tagatttatc
      181 agagattatt gtaacactac ttaaattgtt ttcaaaagga taccctgtaa atggatttaa
      241 catatgatga tatatttttc cgtctttttc gataaatctt tcataaattc cagaagtaac
      301 aacagactta tttgtggttt ctatgtttcc tatggattca ccatttttac tagttgggtc
      361 ttgaatacct attttaaaag gtttatcatt tttatcattt tctaaagtaa atatatttcc
      421 tcctaagttt actaaacctt tttttacatc ctctgatttt aaataggcta caattttatc
      481 agcagcatat cctttagcta tggcacctaa atcaattttc atattttctt tggttaattt
      541 tatagattta tttttttcat ccaatatgat gttcttataa tcaataagag gcagaagttt
      601 ttgtatctca gaatcacttg gaactttagc tttatctgtt ccaatagccc ataaatttga
      661 taatggacca attgttatgt caaaatatcc atctgataat ttggaatatt gtatagcttc
      721 ttttacgacg aaaaatgtat catcagaaac tctgacaaat tttttaccag cattatcatt
      781 tattttagaa acatcacttc ctggtatatg agtgctcatt ttgttgtcaa tatatcttaa
      841 aattgaatca cattcattta agattttatc acttttggat tcttttacat taaatacagt
      901 aacttcgttt actgtaccaa ggtagtagct agttttacta tatttagtat tctggcttga
      961 actcctaaaa aatgaaaata aaattaatgc taaaatcaat atcacaaaaa taaatgtgat
     1021 ttttttctta ttcatggtat attcctccta tgaaattgtg ttattatttt attataacat
     1081 aatttcatag gaggacaaaa aatgaagaaa aaagacattc ttcttatttt aggcattttg
     1141 gttgttatta cagcatgtta tgggataata aatgttataa attctaaaaa tgctggaaat
     1201 attgagattt atgtagataa taaattgtat aaaactgttt caataaatgc caaagaagaa
     1261 tttaaaatag agaatagagg ggggtataac atagtaaaga ttcatgataa aggggttgag
     1321 atagtagatg cttcatgccc tgataaggtt tgcgtacata ctggatttat aaacaagcca
     1381 agtcaaagca tagtttgtat acctaataga gtgagcataa aaataaatac aaatgaaaag
     1441 aatgataatc aagaagatat aatttctaaa taaaataata aaaataattg aaaggaattg
     1501 gtgataatta tggttaactt aggaaacgat tgggatgaat tattgaaaga agaatttgaa
     1561 aaagattact acttaaactt aagaaaattt cttatagaag aatataaaac acgccaaatt
     1621 ttcccaaata tgcacaatat atatgaagct ttaaaacaca cttcatacaa agacacaaaa
     1681 gtcttaatat taggacaaga cccttatcat ggagataatc aagctcatgg tttagctttt
     1741 tcagttcaac cacaagtgaa aactccacca tctctattaa atatgtataa agaattaaaa
     1801 gatgatttag gttgttttat acctaataat ggatatttaa tgccttgggc agaccaagga
     1861 gtgttacttt taaatactgc tcttactgtt agagcgcatg aagctaattc tcataagaac
     1921 aaaggatggg aaattttcac tgatagagta atatctattt taagtgagcg tgaagaccca
     1981 gttatttttg tactatgggg aagtaatgca cgtaaaaaag tagagttgat tgatacaagt
     2041 aaacattata ttttggaagc acctcatcca agcccattgt cagcaagtaa aggctttttt
     2101 ggatgtaaac acttctctaa gataaatgaa attttgaaaa agctaggaaa agaacctatt
     2161 aattggcaga tagaaaatat ataaaagtaa aaggtaacaa aatgataaca aaacaacaat
     2221 aaacaatgac attgttgtaa ctaatagaaa tatgcttaaa tagttgaaaa tactatgtat
     2281 gcatatttct atttttttgt caagttgaca actcttgaaa aaatagttac aatttagtta
     2341 cacaaaaaac ataaataaaa aaagaaactt acaaagcaga attttatgaa gcaaaatata
     2401 tagtgtaaaa aatcttaggg aggaaatttt atatgaattt taaaaaattg agtaatgcta
     2461 aagtctcggt aatggcagtt gttttatcag ttggaatatt atcgggatac tctgcgctta
     2521 ataaacaagt aacattagta gtaaggggag aagaaagaac tatatcaaca tttagttcta
     2581 atgtaggaga ccttttagaa tctcaaaaca ttagctatga tgcaaatgat atagtaactt
     2641 tagatgaaaa tacaaagtta tctaatggag ataaaataga agtaattgat gtaaaggaaa
     2701 aaacagttgt agaaactaaa gacattccat ttgaagttac agttgttgaa gatggctctt
     2761 tattaaaggg tgattctaag gttaaagaag aaggaaagtc tggtaaaagt gaattaatat
     2821 ataaagaaac ttatcacaat ggaaagaaag tagaaaagga atttgtgaaa aaggttgtta
     2881 aagctgagcc agtaaataag gttgtaagca aagggacaaa ggtagaaatg caagtagcct
     2941 catcaagggg agaaagttca agaagaatgg cttcatcatc atctagtagc agtagtagta
     3001 acaatggtaa aaatatgaga gtggtagcaa ctgcatatac tggtcacagt ataactgcaa
     3061 caggaacaaa acctaagtgg ggaacaatag ctgtagaccc taaggttata ccatatggaa
     3121 ctaaagttta tatacctcaa tttgacatgg tgtttactgc agaagactgt ggtggagcaa
     3181 taaaaggaaa taaaattgat atctacatga atgattcaaa aacagtttat aactggggaa
     3241 gaaaaactat agatatacgt atattaaaat agttaaataa tttatatttt tactggaaat
     3301 tatattttga ttatatagag aagaaactct ttatagattc taaaaatata atttccagtt
     3361 ttttattttg gatatgtaat aaaacaggta aattttaacc atgtatagat aaaagctatt
     3421 taaattataa tttactatca tataaatcta taaaaattgt tgcaaagagt caaaatatat
     3481 gatattatag atttcgttga gaaggatttc attatgggag tggatgggtg ccggtgtgcc
     3541 ctctagtctt caaaactagt atgaggggtt aagagcctct taggtgggtt cgattcccac
     3601 gcattcccgc caatatgtaa aaggtatctt ttaattaaaa gatacctttt taaatttatt
     3661 ttaagataat ttatttttaa taatatataa tgttttgtat tatataatgt aaattttcta
     3721 atactattat atatttagat aaaaatctag gagtgtggaa agatggaaaa taattttaca
     3781 acgagaacta gttttctagt tggagatgat gggataaaaa aacttaataa ttcaaatata
     3841 atagtatttg gagttggagg agttggtagt tttactgttg aagctttagc aagagctgga
     3901 gtaggcaata ttactattgt agattttgat gatgtagata taaccaatat aaatagacaa
     3961 ataccagccc tacattcaac tgtaggaaga tacaaagttg atgtcatgga agaaagaata
     4021 ttagatataa atcctaatat caatataaaa aaaataagaa gtctttataa taaagatact
     4081 agtgacgaaa tactaactga aagatatgat tatgttgtgg atgcgataga catggtaagt
     4141 tccaaaatac atctaattga aacttgtgaa aaaaaaggat tgaaaataat aagttctatg
     4201 ggtatgggaa ataaattaga cccaactaaa atagtcgtta cagatataca caaaacaagt
     4261 acttgtcctc tagctaaagt tatgaggaaa gaacttaggg atagaggtat caaaaagtta
     4321 aaagtagtat attctactga acaaccaata gaacttaaga agaaagtaat gaatggtaga
     4381 aaagtaacac ctggtagtgt atcatttgtc ccatctgtag gaggtttaat aatcgcatct
     4441 gtaatagtaa atgaactatt aggacaataa gttttaaata ttttaattta taaattttat
     4501 atacaaattt gaaggctaga aggatgaata taaagcaatt ttatattcat acttttagcc
     4561 tagttatttt atattacctt attttatgta gttgtagctt tattttttag attatgtatc
     4621 tatttcataa ttatattttg taaatataat ttctccatca ctataaaaaa caatatctcc
     4681 tattctgtct gttctataaa ttgatacatg gttttgtttt agattattaa taacctccct
     4741 atgtggatga ccatattgat ttttatatcc acatgatatt gcagctatat ctggactaat
     4801 tttttttata aattctaaag atgaagaaga attacttcca tgatgaccta tttttataaa
     4861 attaatatta tttaatttaa aagaatgtaa aatttctttt tcattttctt cttcagcatc
     4921 acccataaat aaaaaagaat tatcattaaa agttaatttg aaaactatag aatttaaatt
     4981 agattcttct tgtatatatg atggacttaa tacatatata tttatattgt tatctatgtt
     5041 taaaacatca tttttataga gatgctgaat agataagttt ttatccgtac aagaatttat
     5101 tagattttga taagcctcac tatcagttga ttgttcaggc atatatatgg agtttacatt
     5161 aaattttttt ataatattat caagacttcc tatgtggtct gaatctggat gagttgctat
     5221 tataatatca atagttttta ttcttttttg tctaagataa ctaataatta tattttcgct
     5281 atcttcatca cctccatcaa ttaatatatt cttatttgtg ggtgtttgaa ctaaaatact
     5341 atcaccttgt ccaacatcaa tcatatgtat agatagtaga gattttttgt cacagccgat
     5401 taaaagtgat attataataa gtattgataa aaaaatttta tttctcaata aaaaatcact
     5461 ccattcatta catgtttata taaatttatg atgggtaata taagatatat atgacttatt
     5521 gtattttata ttttaaacaa tataaatgaa attaatacca taataaaatt ggagtttttt
     5581 atatatacaa aataaaattt ttaaaattaa gcaataaaaa aggtaggagg attttatgtt
     5641 tttcaaagat attgaactaa attcaaaaaa agagttagac ccatattttg atttagtaga
     5701 ttatgaagct tgtgagtact gttttagtac tttatatatg tggcaacatg tttataagac
     5761 aggatattat ataggtgaag attttgccgt tttagttggg gaatacgaag gagatagctt
     5821 ctcaatatta ccacttgcta aaaaagataa acttccagaa gttgtagatt ttgtccttga
     5881 atatttttct aaaaataata aaaaaatata cttaagaggt attactactg aagtagtaga
     5941 gtttttaaaa gaaaaatatc caggtagatt tgagtatata gaagaaagag atttatttga
     6001 ctatatttat gatgcagaaa gtttaagaac tctagctggt aaaaagaatc aaaagaagag
     6061 aaaccatata aactatttct taaaagagta tgcaggtaga tatgaagcta aattattaga
     6121 taaagaaaac tttgatgagt gtttagtact aatgaaggaa tgggaaagta ataaggaaga
     6181 aaataatgaa tttgatgaaa gtatggatga tgaattaata ggtatcaaaa agatatttaa
     6241 ccattatgac atattaaaag ataaggtaaa agtgtttggt gtttatgtag atggaaaatt
     6301 agaagctttt agtataggag agcttttaaa tccaaatatg gcattgattc atatagaaaa
     6361 agctaatccc gatataagag gtctgtatcc atttataaat caacaatttt tggtaagtga
     6421 atttaaggac gttgagtttg taaatagaga agaagattta ggtattgaag gattaagaaa
     6481 agctaaactt tcttatcatc cttgcagatt tgttgaaaag tacagtgtta gggaggctta
     6541 atagactata tggagattag gtatgctaaa gaagaagaga tagaaagtat aaaagagatt
     6601 tggagttatt gttttaacga tacggagagt tttatgaagt actattttaa tgataaatat
     6661 aaatctgaaa atactgtagt agctttagat gaaggaaaaa ttatttcttc tcttcagcta
     6721 aatcagtata agttattact aaatagcaag gtatataata catcatatgt agtaggagtt
     6781 tctacacttc cagagggaag aggagctgga tatatgaaca aagtaatgaa atttacttta
     6841 aacgaattgt ataaaaaagg gcaattagtt tctatattga tgcctattga ctatagactt
     6901 tatagaagat ttggatatga gcattgttac gaccagattg aatatactat taacacagat
     6961 gatttaaaaa attttaagtc aagtggaaaa atgataaagt ctaatttatc acaaattgat
     7021 gatttgatac aaatagatag aactttttta aatgaggtta atggtaatgt gttaaaagat
     7081 gaacattatt atgaaaactt atttaaagag attcaaagtg aagatggatt tttatacatt
     7141 catgaaggaa atgaaaaaga tggatatata gtttattttc ttcaggaaga taaaatgttt
     7201 gtcagagagt tattttataa aaatatagat gcccttaaat caatgcttaa atttatatat
     7261 aatcacaata ctcaatgtaa gatagttact atatctactc ctacaataga taagataaga
     7321 tttatattag acaaccctaa agatagtgat ataaaaataa aaccttttat gatgggaaga
     7381 gttataaatg taaagaagtt tattgaagat atagatattg agaatgatat taatagctca
     7441 tttaatttat taatagaaga caagtttata gatgaaaata atggtctatt taaaatttct
     7501 atacagaata agaaggtatc tgtagagcag ttagataaaa aaggtgcaga aaagcctcaa
     7561 gaagattttg atattaaatt agatataaat accttaacac agttatcatt ttcttatata
     7621 gatgtaaatg aagctatttt cttaaatgat ataaaagatg tttcagaaga aacactagaa
     7681 acacttaatt gtattttttc taaaaaaaat aactatataa atgaatatat ataggtagag
     7741 tacgttaaaa gattttaaaa atcttttgac gtattttttt gtttagataa caatttatct
     7801 tgtttaaaaa ttttctattt gaaaataatc taaataatat acttttatat gggagtgatt
     7861 ataattcaaa taagaagacc attattaatt atattaatgt ttgtggtagc tatatcattt
     7921 atcattacaa ataacacaaa aaacgacgaa gaacttaatg ataagataat aactatacaa
     7981 ggagttgtca aaggaaaaat tgaaaagaaa agatacaatc agtacaaggt aggggttttt
     8041 ctaataaatg attatacaaa aaataaaaat ttgaagattg gtcagaaagt taatattaca
     8101 ggtaaattca aaagtttaga taaaatgaag tatgatgatt ttgattatgg aaggtatata
     8161 aaaagtactg gatataaagg tatagtgtat ataaataact ataaaattat tgataaaaat
     8221 aaactttact ctttaatagg ggaaataaag ttttatatta gtaaaactta taggtactta
     8281 tataagaaaa actcagattt tataaattct atactattag cagaagttga aaatttgact
     8341 gaggaacaaa aagaaatttt tactagaact ggaactagtc atgtaatatc tatatcaggt
     8401 cttcatacag gaatattatg tgtaattata tcctttctat taagaggtat aaataaattg
     8461 tataaattat taatactgtc gatttttata actttatatt gtataatggt aggtgcgtct
     8521 ccatccataa ttaggtctat agcatttgtt atgatttttt atttatcaat atttattgat
     8581 agaaagaaag atggaatatc ggcattgtct ctaattggga taattttaat tatgaataac
     8641 ccctatgtta tatataatgt aagttttcaa ttgtcttttt tagctacact ttctatatta
     8701 atattctata ataaaataaa tagtattata aaattaagta tggtatcatt gacaatatca
     8761 tcaaatatat taactttacc tataatttat tatacattta aaggtatacc attgctttca
     8821 attataggaa atttaattat agtaccattt gtgggagtaa taatgtattt aagtattgca
     8881 agtttaatcg tatttaaagt aagtgtagta atagctaaaa ttatatcttt ttttaatagt
     8941 acattgatag aaagtatatt ttttttacta gaaaaaatca gcaatttaag ttttgcatac
     9001 attaatatag aaaatcctaa attttatatt gttgttattt attacatagg agtgtttttt
     9061 tacatatttt acatagaagg aaaagagata aaggagcaag agaatgaatt acaaggatat
     9121 tataaggaat gtaaaagaga aaaactttga aaaaatgtat cttttttatg gaagagagta
     9181 ttatttaatt gagaatgcaa taaaagcatt taaagatagt ctaaacgagg gaatgcttga
     9241 ttttaactta gatataatag atgggaagga aatagtctta aatcagctta taagctctat
     9301 agaaacactt ccatttatgg atgatagaaa gatagtaata ataaaagact ttgaactatt
     9361 aaaaggaaag aaaaagaatt ttacagatag tgatgagaaa tacttaatag agcatttaga
     9421 caatattcca gacaccacaa ctatagtttt tgttgtatat ggagatgtag acaaaagaaa
     9481 atcattagtt aagaaaatag gaaacaatgg aattgttttt gattgtgata agctttctga
     9541 tatggatttg tttaaatgga taaaaaaatc ttttgcttta aatgatgtta taatagataa
     9601 ttctcaaatc atgtatttta ttgaacaaga aggatacaga gataagagta gtgaaaagac
     9661 attatctgat ttagaaaatg aaataaataa aataagttca tttgttggta aagggaataa
     9721 tgtaaccaat gatgtaattg ataaattatc tcaaaaaaaa gttgagaatg atatattcaa
     9781 attaatcgat tatattggag aacaaaatgc ttctaatgca atgaaaatat taaatgatat
     9841 gatacaagaa ggcgaatctg tattaggtat attttctatg atagcaagac aatttaaaat
     9901 aattatgcaa gtaagacagt tacaacttga tggatattca acaaaactta tagctgataa
     9961 attaaaaatg catcaatttg ttgtaggaaa agcattaaag caaactaaaa atttttctga
    10021 tgatataatt gtggaaatat taaactatat attagaaagt gattacaaaa taaagacagg
    10081 tcttataaga gatactttag cagttgaaat gttggttagt agatactgta aaagagaagc
    10141 aatttaaaaa atctatatat taataaaaaa taaatagcaa gatatgttat agttcagagt
    10201 agatgtaagc taaattaagt ttgatataca atcaactata atatgtctaa ctatttattt
    10261 tttattgtat aaatttacaa aataaaaaac ccctacatag gggttattat atataagaaa
    10321 tataaactgg taattaagcg ttcataccgt ttaatttttg agctaattta gctacttttc
    10381 tagctgcagc attcttatgt atagttcctt tagaagctac ttggtatatt cttttttgag
    10441 cgtattggaa cttagcaaca gcatcctctc tgtttccagc tgcaacagca tcttcgaatc
    10501 ttcttattgc agtttttatt tgagattttc taactctatt taaagcagtt ttcttttcga
    10561 taacacttat tcttttctta gctgatttta tatttgccac cgtgttcacc tccctgtatt
    10621 tttttatcat attcatcgtc aacatattag attttaacag aaataaatct aaaaatcaag
    10681 gattattatt tatatatttt aataatgttt tttattttga ttttttagac tgtaaattgt
    10741 gtataaataa tttgcaatat cttgttttta aattggtata ttttgtgttt taaatatttt
    10801 gacatatata aattaatatt ttatttttgt aacaaatatt ttgagataat acaaactatt
    10861 aacatacatt ttgatagtat ttcc
//
LOCUS       DAFKMX010000133        10791 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFKMX010000133 DAFKMX010000000
VERSION     DAFKMX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.133"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            152..1993
                     /locus_tag="LZB08_002717"
     CDS             152..1993
                     /locus_tag="LZB08_002717"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7316208.1"
                     /translation="MRILKIINDLENTNKYLSIAYFIDKYSVSKRTLQNDFSYLSKVS
                     SNKGFKIIQKRGLGYLLEIEDNTLFKKFKVELFEYLDQPKLNVESIIACLILNEDYIT
                     IDRILEELKTSRSVVKGYKEDIDSYLKKYNLKLERKSHYGLKIINSMKERRKLIVELY
                     IKENEIIKKYIDSKLENDFKEVQEKFINKLKENNLVINYIELKEILSWLKVTMYIDRQ
                     SKLLELDDSTNEYELNLSSEDYKILKSLIKKKARKKFRRQKGADNLRDDLEDFLKQFD
                     EENNTYFNEDEEFKRSLLNHLTELLERSTFSISYQNPLIDEIQIKYPVAFNIAILLGK
                     IMYKKYNVEIQRDEIGFIATYFAMNMEKQAIYKFRKYNSIAIVCSSGGGSAQLIKFKI
                     SSLFENTNIKTFSLIQMKELEKFNPDIIFSICELDIKLEVPIVYIKELLDDYDILRIK
                     ELIMFEKFKEYSLENKGQAFICKFLRNDYFNIDNDSIDYIKCLEDMSKNIELKGAGNY
                     GYSENVLKREYFATNIYINGVAIPHPIDIDGNENLISVKILQNPIEFKGKKVSLIFMI
                     CLKKENIGLYKKISSSLYEIMNDIETVQLLVNAKTFKEFVALINQRI"
     gene            2005..3033
                     /gene="ypdE"
                     /locus_tag="LZB08_002718"
     CDS             2005..3033
                     /gene="ypdE"
                     /locus_tag="LZB08_002718"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBM7316209.1"
                     /translation="MSIDYLKKLCEADGIASNEQEIRDLLAENINGEISFDNLGSMIV
                     HHRGNGLKMMFCAHMDEVGFMVRHISDIGMLHLINVGGVKDSAKSFQKVNISKVDGTK
                     VKGLINCSYDENNKVKDLYADIGVSTAKEVEDLGINIGDMVCFASESEMLNENVLMAK
                     ALDDRIGCYILKEIDRRVNQIKKNDNDIYLVYTSSEEVGIRGGKTTSKVVNPDIVFAV
                     DVACAPDLIRNYTNQRQLSKGCMIVHYDKTMIPNKKFLNYMKDLATKYDIKFQCDMFS
                     GGGTDAGNAHLANEGKLAIVLGVPLRYCHGNYSMVDMRDVEEVINLCTAVINDMNMEK
                     YNNILKFI"
     gene            3051..3371
                     /locus_tag="LZB08_002719"
     CDS             3051..3371
                     /locus_tag="LZB08_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBM7316210.1"
                     /translation="MENLEMKIMEIIVSSGDSKSKSFEALNKVKEGDYEGARACIEEA
                     RKIDIEAHNAQTALIQSELSGDCESKSMVSLLMVHAQDHYMTSQLSRDLIEKLIDIFE
                     SKEA"
     gene            3374..3700
                     /locus_tag="LZB08_002720"
     CDS             3374..3700
                     /locus_tag="LZB08_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7316211.1"
                     /translation="MRIILCCAGGFSTTMLMKNMENVVKNSKKLNDDDFEFKAIPVDL
                     LKNEVDNCDVLVIGPQIAHKLDYIKPILEPKNVPYVIIDQNTYGKMDGATALKMALIA
                     HKKANM"
     gene            3739..5076
                     /locus_tag="LZB08_002721"
     CDS             3739..5076
                     /locus_tag="LZB08_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBM7316212.1"
                     /translation="MFKKFEQFMNKHLTPIAQRMDKQHHLSAIKKSMVAMTPILIIGS
                     LSLMPEAIGNLTEKAIGANAITSFITNNMDSLQIPFYLTIGMMGFLVCMCITYFLSEH
                     YKLYVPGAMSLAAVGFLLMSFNFTEDGGMDIKYMGTKGLFIAMVAGVLAVELYRWCKK
                     KNFTIRMPESVPDFVSSSFELIPTTVIIVGVFIGIRIITLQVFGVLPPEILTQFLAPL
                     VGSLDNPFTFAFITFLGITLFFFGIHPSVLSPITAPITAQFLAENLEAMAVGQSLPHF
                     YTGGMVSAFANFTGTGVTFGLVFWYLMSKRVDFKRIGQVSLVPALFGINEPILFGGPI
                     VLNPTFFLPFVIGGTILGTFPAFLMNAGILAKPFFNPPYLGVFIEGFLVNGHWLTPIV
                     QAFQMVGSILIWYPFYKMYEKQCDERDTKQVQEIQEESVISPEDLGLLGDLDF"
     gene            5297..6478
                     /locus_tag="LZB08_002722"
     CDS             5297..6478
                     /locus_tag="LZB08_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7316213.1"
                     /translation="MKHNFDEVYNRKGTYCTQWDYIQDRFGKKDLIPFSISDTDFTVP
                     KPVYEKLQEILQHQIYGYSRWNHNDFKGSITGYYNRRFEAKIESDWVLYSPSVMYSVA
                     LLLRLLCNNKKKVATFNPMYDAFFSVIEENGFELEKIQLYEKDGKFNIDFDEFEDSVK
                     NCDVLLLCSPHNPTGKVWKEVELEFLINTCKKYNIKIISDEIHSDIIMPDNKHLPIVK
                     YLREYKELYLVSSGSKTFNYPGLIGSYAVIANKNIYDEFLSITRRRDFLNSVSLPGMY
                     ATMVSYNECDYYIEQLVEYIDNNLSYVSQFIKENFEDIKFTKPEGTYLAWIDCRDVPF
                     TCEEIQDSLINIGGVGIMRGEVYGSEKYLRLNCGCPREKLKKGLERFKQSIDYLYFNN
                     K"
     gene            complement(6626..7348)
                     /locus_tag="LZB08_002723"
     CDS             complement(6626..7348)
                     /locus_tag="LZB08_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316214.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            7739..9166
                     /locus_tag="LZB08_002724"
     CDS             7739..9166
                     /locus_tag="LZB08_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBM7316215.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            9159..9761
                     /locus_tag="LZB08_002725"
                     /pseudo
     CDS             9159..9761
                     /locus_tag="LZB08_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
     gene            10388..10501
                     /locus_tag="LZB08_002726"
     CDS             10388..10501
                     /locus_tag="LZB08_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7316216.1"
                     /translation="MIMGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            10503..>10791
                     /locus_tag="LZB08_002727"
     CDS             10503..>10791
                     /locus_tag="LZB08_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316217.1"
                     /translation="MIAVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKKK"
BASE COUNT         4251 a         1151 c         1872 g         3517 t
ORIGIN      
        1 aaacctttta aaggtattgt atagaaaata taataacagt ataagcatct tacacaaatc
       61 gatactagat tgtacaatta aaataaaata gttctacaat attttttaga atataaaaat
      121 aaaactgtta tggataatag gaggtgtaag aatgcgaata ttaaaaatta taaatgattt
      181 agaaaacaca aataagtatc tatctatagc ttattttata gataagtatt ctgtatctaa
      241 aagaacacta caaaatgatt tttcgtattt atctaaggta tctagtaata aaggttttaa
      301 aataatacaa aaaagaggat taggttatct attagagatt gaagataata ctttatttaa
      361 aaaatttaaa gttgagttat ttgagtattt agaccaacct aagttaaatg ttgaaagtat
      421 aatagcatgt cttatattaa atgaagatta tataactata gataggatat tagaagaatt
      481 aaaaacaagt aggtcagtag taaaaggata taaagaagat attgattctt atttaaaaaa
      541 atacaatctt aagctagaaa gaaaatctca ctatggattg aaaattataa atagtatgaa
      601 agaaagaaga aaattgatag tagagcttta tataaaagaa aatgaaatta taaaaaagta
      661 tattgatagt aaactggaaa acgattttaa agaagttcag gagaaattta tcaataaatt
      721 aaaagaaaat aatttagtca taaattatat tgaattgaaa gaaatactgt cttggttaaa
      781 agtaactatg tatatagata ggcaatctaa attattagag ttagatgata gtaccaatga
      841 gtatgaatta aatttatcta gtgaagatta taagatattg aaatctttga taaagaaaaa
      901 agctagaaaa aaatttagaa gacaaaaagg tgcagataac ttaagagatg atttagaaga
      961 ttttttgaag caatttgatg aagaaaataa tacatatttt aatgaagatg aagaatttaa
     1021 aaggtcatta ttaaatcatt tgacagaatt attagaaagg tctactttta gtatatctta
     1081 tcaaaatccg cttatagatg aaatacaaat caaatatcca gttgctttta atatagctat
     1141 attactagga aaaataatgt ataaaaagta taatgttgaa atccaacgag atgaaattgg
     1201 gtttatagct acatattttg ctatgaatat ggaaaagcaa gctatctata aatttagaaa
     1261 atataattca atagcaatag tatgttcatc aggtggtgga agtgctcagt taataaaatt
     1321 taagataagc agtttgtttg aaaatactaa tataaaaact ttttctctta tacagatgaa
     1381 agaattagaa aaatttaatc cagatattat cttttcgatt tgtgaactag atataaagct
     1441 agaagtacca attgtatata taaaagaact tttagatgat tatgatattt taagaatcaa
     1501 agagttaatt atgtttgaaa aatttaagga atactcttta gaaaataaag gtcaagcatt
     1561 tatctgcaaa tttttaagaa atgactattt taatatagac aatgattcaa ttgattatat
     1621 caaatgttta gaagatatgt ctaaaaatat agagttaaaa ggtgcaggaa attatgggta
     1681 ctctgaaaat gttttgaaaa gagagtactt tgcaacaaat atatatataa atggagttgc
     1741 aatacctcat cctatagata ttgatggaaa tgaaaattta atttcagtaa aaattttaca
     1801 aaatccaata gagtttaaag gtaaaaaagt ttctttaata tttatgattt gtttaaaaaa
     1861 agaaaatatt ggactttata aaaaaattag tagtagttta tatgaaataa tgaatgatat
     1921 agaaactgtt caactacttg taaatgctaa aacattcaaa gaatttgtag ctttaataaa
     1981 tcaaagaata tagagggtaa tcgtatgagt atagattatt taaaaaaact atgtgaagca
     2041 gatggaatag cttcaaatga acaagaaata agagacctat tagctgaaaa tataaatgga
     2101 gaaattagtt ttgataactt aggaagtatg atagttcacc atagaggtaa tggattaaaa
     2161 atgatgtttt gtgctcatat ggatgaagtt ggatttatgg taaggcacat aagtgatatt
     2221 ggaatgctac atttaattaa tgttggtgga gttaaagata gtgcaaagtc tttccaaaaa
     2281 gtaaatataa gtaaagttga tggtactaag gtaaaagggc ttataaattg ttcatatgac
     2341 gaaaataaca aagtaaaaga tttatatgca gatataggag taagtacagc taaagaagtt
     2401 gaggatttag gtataaacat aggagatatg gtatgttttg ctagtgagag tgaaatgtta
     2461 aatgaaaatg tacttatggc aaaggcttta gatgatagaa taggatgcta tatacttaaa
     2521 gagatagata gaagagttaa tcaaataaaa aagaatgata atgatatata tcttgtatat
     2581 acaagttctg aagaagttgg aataagagga ggtaaaacaa catcaaaagt agtgaatcct
     2641 gatatagtgt ttgctgtaga tgttgcttgt gcaccagatt taattagaaa ttataccaat
     2701 caacgccaat tatctaaagg ttgtatgata gtgcattatg ataaaacaat gattcctaat
     2761 aaaaaatttt taaattatat gaaagactta gcaactaagt atgatataaa attccaatgc
     2821 gatatgttta gtggtggagg aacagatgct ggaaatgcac atctagcaaa tgaaggaaaa
     2881 ttagctatag ttttgggggt tcctttaaga tactgtcatg gaaattattc aatggtagat
     2941 atgagagatg ttgaagaggt tatcaattta tgtactgctg ttattaatga tatgaatatg
     3001 gagaagtaca ataacatatt aaagtttatt taacgacata ggaggtaagc atggaaaatt
     3061 tagagatgaa aataatggag ataatagttt cttcagggga tagtaaatca aagtcatttg
     3121 aagccttaaa taaagtgaaa gaaggcgatt atgaaggagc tagagcttgt atagaagaag
     3181 ctagaaaaat agatatagaa gctcataatg cacaaactgc attaatacaa agtgaattat
     3241 ctggagattg tgaaagtaag tcaatggtga gtttattaat ggtacatgcc caagatcatt
     3301 acatgacttc acagttatct agagatttaa tagaaaaatt aatagatata tttgaatcta
     3361 aggaggcgta aaaatgagaa taatattatg ttgtgcaggt ggattttcga ctacaatgct
     3421 aatgaaaaat atggagaatg ttgtaaaaaa tagtaaaaaa ttaaatgatg atgattttga
     3481 atttaaagca ataccagtag atttattaaa aaatgaggtt gataattgtg atgtactagt
     3541 tataggacca caaatagctc ataaacttga ttacataaaa cctatactag agcctaaaaa
     3601 tgtaccatat gtaataatag atcaaaatac atatggaaaa atggatggtg caactgcact
     3661 taagatggca ttaatagctc acaaaaaagc taatatgtaa tattcattaa agtatactat
     3721 gatgatgggg gaaaaataat gtttaaaaag ttcgaacaat ttatgaataa gcatcttaca
     3781 cctattgcac aaagaatgga caaacagcat cacttaagtg caattaaaaa gtcaatggta
     3841 gcgatgactc caatccttat aataggaagt ttaagtctta tgccagaggc tataggaaac
     3901 ttaacagaaa aagctatagg agcaaatgct ataacttcat ttataacaaa taatatggat
     3961 tcattacaaa ttccatttta cttaactata ggaatgatgg gatttttagt atgtatgtgt
     4021 ataacgtatt tcctttcaga acactataag ctttatgtac caggagcaat gtccttagct
     4081 gctgttggtt ttcttttaat gagttttaat ttcactgaag atggtggaat ggatattaag
     4141 tatatgggaa ctaaagggtt atttatagca atggtggcag gtgtattagc tgttgagttg
     4201 tatagatggt gtaaaaagaa aaactttact atacgtatgc cagaaagtgt acccgatttt
     4261 gtatcaagtt catttgaatt aatacctaca acagtaataa tagttggagt atttataggt
     4321 ataagaataa taactttaca agtatttgga gtacttcctc cagaaatact tacccaattt
     4381 ttagctccat tagttggttc tttagataat ccatttacat ttgcatttat aacatttcta
     4441 ggaataacat tattcttttt tggaatacat ccatctgtat tatcgccaat aacagcacca
     4501 ataacagctc aatttttagc tgaaaacttg gaggctatgg cagtaggtca atcattacca
     4561 cacttttata caggaggaat ggtaagtgca tttgcaaatt ttacaggtac aggagtaaca
     4621 tttggcttgg tattttggta cttaatgtct aagagagtag atttcaagcg tataggtcaa
     4681 gtttctttag taccagcatt atttggtata aatgaaccta tattatttgg tggtccgata
     4741 gttttaaacc caacattctt tttacctttt gtaattggag gaactatact gggaacattc
     4801 cctgcatttt taatgaatgc aggaatatta gccaaaccct ttttcaaccc tccatattta
     4861 ggagtattta tagaaggatt cttagtaaat ggacattggt taactccaat agttcaagca
     4921 tttcaaatgg tgggttctat attaatatgg tatccattct ataaaatgta tgaaaaacag
     4981 tgtgatgaga gagatactaa gcaggttcaa gaaattcaag aagaatctgt aatatctcca
     5041 gaagatttag gcttattagg tgatttagat ttctagaaat ataatcaagc atgtaaataa
     5101 aagctgtgga gatagagtta caaatctata ttcatagctt ttattttata gagtagaaag
     5161 atattttagt aacaaaatat aattaatatt ttattttaga taaaaaacat gcaaatgaag
     5221 caaaaaatac tccataaaaa tctaataaat cgtattttta tattgtatgt gagaagaatt
     5281 atattggagg attaatatga agcacaattt tgatgaagtt tataaccgca aaggtactta
     5341 ttgtacacag tgggattata tacaagatag atttggtaaa aaagatttga ttccattttc
     5401 tatttcagat acagatttta cagtacctaa accagtatat gaaaagttac aggagatatt
     5461 acagcatcag atatatggat attctagatg gaatcacaat gattttaaag gaagtattac
     5521 aggctattat aatagacgat ttgaagcgaa aatagagagc gattgggtgt tatatagtcc
     5581 atcagttatg tattcagtag cattactact tagattactt tgtaataata aaaagaaagt
     5641 tgctacattt aaccctatgt atgatgcatt tttcagtgta atagaagaaa atggatttga
     5701 gttagaaaaa atacaattat atgaaaagga tggtaaattt aatatagatt ttgacgaatt
     5761 tgaagatagt gtaaaaaatt gtgatgtatt attattatgc tcacctcata atccaacagg
     5821 taaggtttgg aaagaagttg aattagagtt tttaataaat acttgtaaaa aatacaatat
     5881 aaaaattata agtgatgaaa tacattctga tataatcatg ccagataata aacatttgcc
     5941 aatagttaaa tatttaaggg aatataaaga actatattta gtgtcttctg gaagtaaaac
     6001 atttaattat ccagggttaa taggttcata tgctgttata gcaaataaaa atatatatga
     6061 tgaattttta tcaataacaa gaagaagaga ttttctaaac tctgtaagtt taccaggtat
     6121 gtatgcgaca atggtttctt ataatgaatg tgattattat atagaacaac tcgtcgaata
     6181 catagataat aacttaagtt atgtatcaca gtttataaaa gagaattttg aagatattaa
     6241 atttacaaag ccagaaggta cttatttggc atggatagat tgtagagacg ttccatttac
     6301 ttgtgaagaa atacaagatt ctttaattaa tattggggga gtgggaataa tgcggggaga
     6361 agtatatgga agtgagaaat atttaagatt aaattgtggc tgtccaagag aaaaattaaa
     6421 aaaaggtctt gaaagattta aacagagtat agactattta tactttaaca acaaatagtt
     6481 taaaaaataa actattaaaa atagataggg acaatttgtt cctatctatt tttcaattga
     6541 taaaaaattg atttctaaaa tgaataattt taatttggta aaataaaaaa agatgattca
     6601 aatatttatt ttgagacatc ctcttttaat atctttgagt aacatccatg aatacaaatg
     6661 tttcaggtgt atgctttgat tcagtatatt caaaaagctt tccatcatca gtatatacac
     6721 tatttttaat aacaccaaca caattataat catttagagg tagcgttttt aactcaagct
     6781 ctgtagcttt ttcaatttta aatatctttc tggatgaaac aatttttgta cctagacact
     6841 cttctatata tttataaata gagccttgtg ctatagaaac atcaaggtct ttaactatac
     6901 tacatagaaa atagttaatg tcgagaattt taggtatgtt atcaatatat cttagtctgt
     6961 gaaggtaata tacttcacta ccaactttaa aacctgtctt tttagataac ttattatcaa
     7021 ttaagatttt gctgaagtgg agaacactag tagcagtatt aaccttttta tcttctacaa
     7081 tcgattctac acctttgaaa ttgtgcatat ttagaaaaaa atcattagaa caactatttt
     7141 ctaacactac aacacccttg ccttttatac tttgaacata tccttctgtg tttaattgag
     7201 aaattgctct acgtacagtg tttctgctac atgaaaacct ttcaataagt tgaagttcag
     7261 taggcagcat catattactt gtatatttac catcaagtat ttcctctttc aatacttcat
     7321 atatctctga aaatttattt ttagccatat acccctatcc ctccttaaag gtctttagta
     7381 atattattga actgatatga acaaattata tcaaatgtgt ttagacatgt atagtgaaaa
     7441 tagaatcata tcaaccataa aaaggacaaa atagaattat tttgtgggtt gtttcaaata
     7501 catggttaaa catattttta aaataaaaaa aatgaattaa aaaaatagac tagatatagc
     7561 aatacactta aaaggattta tatgttaaac taaatgataa aaataaaatg aataaaaaat
     7621 attgacaatt tgtttaaaca aatataatat ttaaaatata aaatgtgaat aaatcgtttt
     7681 cacaaaaaat taaaccgaaa tagaaagggg attggatatg aagaggttct taaacaaaat
     7741 gatgcaacaa ctgtcacttt taggaaagac tatgctggtg ccaatagctg taatgccagc
     7801 agcaggtata ttaggattat tatttggtcc aaatatgctc aatgtgccag ctatatctaa
     7861 tatatctaat atagtatttt caaatgtgga ctacttattt ttattaggag cagctagtgc
     7921 ctactcaaag tctaaagaca agacaagtgt aatggttgca gccgtagttg catatatgat
     7981 atttaagtca aatttagagt tattgaatga tacattaaat gcaggtgtat ttggtgggat
     8041 aattgttgga gcttgtatag catggactta caataaaaca tataaagtta aagttccagc
     8101 atttttaagc ttctttgcag gagaaaaatg tgtaattaca ctatcaccag tggttgcttt
     8161 aattttatca tatatattta gtttattatg gatatatcca caagctgcaa tgaatcaatt
     8221 tggtttttgg ttgggaggag ctggtgcaat aggaatattc ttgtttttat tctgtaaccg
     8281 tgcactaata ccaactgggt tacatcaagt tttaaatgct tacatattat ttgaaatggg
     8341 tgaatataca acatcaacag gagaagttat aagaggagaa attcctagat ttatggcagg
     8401 agacccaaat gcaggattct tctggacagg attttacata attatgatgt tttcaatacc
     8461 tgctatttct tatgctatat ataaaacagc acaagataaa aagaagcaag aagtaaaagg
     8521 tgcaatgacc gctggagcat tgacttcttt tatggcaaca gtaactgaac caatagaatt
     8581 ttcgttttta tttgcatctc caaagttata tataattcat tctttttatt ctggtctagg
     8641 tggagttgta ctatatttac tgggtgctcg cttgggttca tttaatggag caagtataat
     8701 tgactatgtc ttaagtttta gttatggaga taaagcatgg ttagttatac ctgtaggagt
     8761 tgtattcttt gtattatatt atgtaacatt taaatatata ataataaaag ataatgtgca
     8821 aacaccagga cgtgaagttg agatagagat aggtgataaa gttagtgaaa aagaaaagaa
     8881 tttaaaactt tctcatggta atttttcata tatggctaaa cagataatta aaaattgtgg
     8941 tggttatgaa aatatagtga ctcttaataa ttgtatgacg agattacgtt tagaagtgaa
     9001 ggatgctact attttgaatg atgataatat taaaaaaaca ggagccaaag gggtaattaa
     9061 attgagtaat acatcagttc aaatcatcat aggaacggat gttgttaaag taaaggatga
     9121 gatggaaatg caacttgatg agttgagaaa gcaggcagat gcataaaatg aagaaattag
     9181 tagttagggc agatgatata ggatattcag aaggtgttac actagggata atatcttctt
     9241 ataagaacgg tattgttaca agtgctggaa tcatggtgaa tatgcctttt tcaagacaag
     9301 ctatagaaat ggtaaaaaat gatgatgtat gttttggttt acatgttaat ttagtaatag
     9361 gagaaccttg ttgcaaagaa catattaaga taaagaattt gttagatgaa ggtggaaaat
     9421 ttgtttctag caaaataaga agaacgcaac tactgacagg taaagaggtt tttaattatg
     9481 aagaagtata tgaagaagtt tgtgctcaaa ttgaaactta caagttgtat atgaataaat
     9541 tgcctgagta tattgatata catggtatag aagaagatgt atcaatatca gcaatgtgtg
     9601 atgctgcaaa agaatatggt ataccaagtt gtccctatta tttggattca tgtattagca
     9661 tgccaagtaa atatcctcag aatgattttt acaagttaaa taaatctttt gttaaattat
     9721 ttgaagaaaa atatataaat ttagataaag atataacatt gattaaaaaa tagctacgca
     9781 cactcgtgac tttagtcatg agttagtagc taaaatagtc agcatatagg gaaacttata
     9841 tgtagagata ggatagaacc tatccaatac tacttgaatt gctaaaaacc cctaaagcta
     9901 accaaactaa aacataagga tgaaataaac ctaagtgtga aagttacgaa agtagaaaaa
     9961 attggttaga tggtataagg ttaaatccta agtactttaa aatgggcaat cagcaggtaa
    10021 gctccgaata ggagaaactt caacgactat tcctcttgag ggaagtacac tacaagctat
    10081 tggtagtgga agtgggtaga ccttagcgga taatgccgaa ggataagata tagtctgtgc
    10141 ttacatgaaa gtgtaagaag ttcataagag aactgcatag gtagtagcga acttatgtga
    10201 acgacaacct caaaaatgat aaaagttaag acttacggtt cttatatatg ttatttaaaa
    10261 ataaatataa aaaacttcaa aatacttgtt ttaagcaatt gtatatttta atattatgat
    10321 aagtgatgaa aaatggaagt tactcatggt agaggatatg catatagtat gtcaaatata
    10381 gacatccatt attatgggga gacatctttg gaatcctagt tatttcgttg ctactgtatc
    10441 agaaaataca gaggagcaga taagaaaata tattaataat cagaaaaaac aagtaaagtg
    10501 aggtgatagc agtggaaaaa gcttataagt ttagaatgta tccaaataaa aaacagcaag
    10561 agttaattaa caaaactttt ggatgttgta gatttgtata taataagtat cttgctaaaa
    10621 gaatagaagt ttataaaaac gataaagaaa cttttactta taaacagtgt agttctgatt
    10681 tgaccaattt aaaaaaagaa ttaaaatggc ttaaggaacc tgacaaattt tcactacaaa
    10741 acgccttaaa agatttagat aatgcatata aaaaattctt taaaaaaaag g
//
LOCUS       DAFKMX010000134        10730 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFKMX010000134 DAFKMX010000000
VERSION     DAFKMX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.134"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            317..775
                     /locus_tag="LZB08_002728"
     CDS             317..775
                     /locus_tag="LZB08_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7316218.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            836..1111
                     /locus_tag="LZB08_002729"
     CDS             836..1111
                     /locus_tag="LZB08_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7316219.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1170..2444
                     /locus_tag="LZB08_002730"
     CDS             1170..2444
                     /locus_tag="LZB08_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBM7316220.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPK
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2574..3626
                     /locus_tag="LZB08_002731"
     CDS             2574..3626
                     /locus_tag="LZB08_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBM7316221.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3626..4657
                     /locus_tag="LZB08_002732"
     CDS             3626..4657
                     /locus_tag="LZB08_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBM7316222.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKAIGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EENLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4749..5561
                     /locus_tag="LZB08_002733"
     CDS             4749..5561
                     /locus_tag="LZB08_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBM7316223.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5564..6484
                     /locus_tag="LZB08_002734"
     CDS             5564..6484
                     /locus_tag="LZB08_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBM7316224.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6659..7105
                     /gene="rpiB"
                     /locus_tag="LZB08_002735"
     CDS             6659..7105
                     /gene="rpiB"
                     /locus_tag="LZB08_002735"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBM7316225.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7135..7800
                     /locus_tag="LZB08_002736"
     CDS             7135..7800
                     /locus_tag="LZB08_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBM7316226.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTD
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7910..9412
                     /locus_tag="LZB08_002737"
     CDS             7910..9412
                     /locus_tag="LZB08_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBM7316227.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9724..>10730)
                     /locus_tag="LZB08_002738"
     CDS             complement(9724..>10730)
                     /locus_tag="LZB08_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316228.1"
                     /translation="AIVFLISIVVQAIIQYLVLIFFMYIYYAEAIAIVILFTLVNTVF
                     HFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLET
                     AVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKS
                     QRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPE
                     YAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNL
                     KPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN
                     "
BASE COUNT         4290 a         1216 c         1967 g         3257 t
ORIGIN      
        1 aataaataat tttataaaat agttgttaaa cataaaaaac catttgtaaa aaacaaaaaa
       61 atcatttctc taacaaaaaa aaccacaaaa aatataaaaa agtatggcta gttaagtgat
      121 taacaagtaa aaattaaaat aaaaatcaaa aaaaatttat caatgaaagt tagctaaaat
      181 cctggaagag agcattattt ttgggaaaac attttctttt gtttcacaaa aaaagccact
      241 ttttggcaca caacatgctt tatataaagt caagaaaaac taaggtataa gacaaaggca
      301 attaggaggt gtaaatatgg agtttataaa ttttgtaagt gataaagtta tagaccttaa
      361 aatggattgt aaaacgaaag aagatttctt taaagaaata cataataaag tttttgattt
      421 aggctttgta aaagaagaat ttggagaaaa aatattagca agagaaaatg tatttccaac
      481 aggtttaaat ttaggtgatt atggagtagc aataccacat acagatgcag aatatattaa
      541 agagcaattt atatcagtct gtacttttaa tgaaccagta gtatttagtt ctatggaaga
      601 ccaagatgaa aaagtacctg taaatttggc tttcgtatta ggacttaatc aaccacacag
      661 tcaattatct gtattaacag aattaatggg aattatgcaa aataaagagt tagttgataa
      721 gttgatgagt tctactgata aagaagaagt tttaaagaca ctaaagtctt tataaaaata
      781 aaaaagtact aaaaataaaa aatataaaaa taaaaattat taggaggaaa taaaaatgaa
      841 aaaaatatta gtagcatgtg gagcaggaat agcaacatca acagtagttt gtgacaaggt
      901 agagagatta gttaaggaaa ataatataca agcagaagtt atacaatgca aaatagctga
      961 atgtgcaaca aagcaagaag gagcagattt aatagtatca acaactatac taccaacaac
     1021 ttataatata ccagcaataa aagcaacagc atatataact ggagtaaata cagcagcatt
     1081 agataaaaaa atattagatg ctttaaaata atcctatcat agatacattg aaataatatt
     1141 ttataaaaca gaaaaaggag gatttataaa tggataaatt attagcagta gtacaataca
     1201 ttttaaatat gggaccgaca gtaatactac caataacaat tgtagtacta ggaatgatat
     1261 ttagaattaa gtttacaaaa gctttaaagt caggtcttac aattggtata ggatttgtgg
     1321 gtataaactt agttgtaaac ctattaacat caacactagg gccagcggct caaggaatgg
     1381 ttgaaagatt cggacttaat ttaacagtaa tagatgttgg ttggccaaca acagcatcag
     1441 caacatgggc ttcaccagtt gcaccaatat taataccagt ttgtttatta gtaaacttac
     1501 tattattata cttcaataaa acaaagatat taaacgttga tatatggaat tattggcact
     1561 ttatagtagc aggtgcagtt ggatatatag taacaggaag ttatgtttgg gctatattct
     1621 gtgcaatact aatggagtta ttagtactgt ttattgcaga aaagacagca aaaaatgttc
     1681 aggagtttta tgatttagaa ggtatagcat taccaactgg ttcaacagct tcatttgctc
     1741 cacttggata tttagtaggt aaatttgttg aaaaaatacc aggaataaat aaaatacaag
     1801 cagacccaga ctctatacaa aaaagatttg gtatatttgg agaaccaatg atgatgggga
     1861 ttatactagg tataatactt ggagcacttg cgggatatga tgtagcagga atattcaatg
     1921 taggtatatc aatgggtgca gtaatgttac ttatgcctag aatggttaga atattaatgg
     1981 aaggtttaat tccaatttca gaatcaataa gagatttctt acaagcaaaa tatggagaca
     2041 gagaacttta tataggtctg gatgcagcag tagcaactgg acatccagca gttatatcaa
     2101 cagctcttat acttgttcct ataactttgt tcttagcagt tatactacca ggaaacaaag
     2161 ttttaccatt tggagactta gcgacaatac cattttatat ggcatttata gtgtgcttta
     2221 gaaaaggtaa tatagttcaa tcagttataa caggaacaat agtaatagct atatcacttt
     2281 atatggctac aaactttgca gatgttcata cacaattatt acaacaagca aatatgatgc
     2341 caaaaggaac aacaatggtt acaagtatag atacaggtgg aaacttgcta aaatggggaa
     2401 tattgaaatt atctcaatta gtttcttcat ttggaatatt ctaatatgta gttaatagag
     2461 attactcttt tttagtaaag tatgcgaaaa tttaacattt agcagctact aaaaaagagt
     2521 aatatttttt ataaattttt aaaaatacaa aatacaagag gaggagattt atcgtgaaag
     2581 cagcagtatt acatggcaca aatgatatga gatttgaaga tatagaaata aaaccatgtg
     2641 aaagtgatga agtaaaaata aaagttatgg cagcaggaat atgtgggtca gatcctccaa
     2701 gagtattaaa acattggaaa tatccagttc cagctatacc aggacatgag ttttctggag
     2761 taattgctga agttggtaag gatgtaaaaa atgtaaaagt tggagataga gtagttgcaa
     2821 ttccttttat accttgtaat gaatgtgaat attgtaaaag aggattattt tcattgtgtg
     2881 atgaccatgg aatgttagga gctaaaagtt ttggagcttt tgcagagtat gtaaatataa
     2941 aagctacaaa tgtacttcca ataggagata tggactttga agatgctgca atgatagagc
     3001 cactagcagt tgctatgcat ggagtactta atataggtgt tcaagttgga gacactgtag
     3061 ccgtaatggg tagtggaaca atggggcagt tggtaataca aggacttaaa atagcagggg
     3121 caggaacaat tatagcagtt gatatatctg ataataaatt aagagaatca aaggaattag
     3181 gggctgatat aataattaat gctaaagata ttaacccagt agaaaaaata aaagaactta
     3241 ctggtggaaa aggtgttgac atagctcttg aatgtgcagg ttcaaaaata actcaagaac
     3301 aatgtttact tataactaaa aagaaaagta agataggatt tttagggata gcatattcag
     3361 atataacttt atcagaagaa gcatttgaaa atatatttag aaaagaacta gaattaaaag
     3421 gtttttggaa ctcttattct gcaccatttc caggacaaga atggactaag ggaatcaatc
     3481 tagttaatga aggaaagata aaattaaaag aaatggtatc tcatagattc tcacttgaag
     3541 atacttacaa agcatttgaa atgataagag atagaaaaga agagtttaac aaaatattaa
     3601 ttttaccaca aggggttgaa aaataatgaa agcagtagtt aaaactaaac caggctatga
     3661 taatgtagaa gttttagaag tagaagaacc aaaagctata ggagacaagg ttaaaataaa
     3721 agtagaatat agtggtatat gtggttcaga tatacattca tttaaaggtg aatatgcaaa
     3781 tataaaagcc ccagtaacat taggtcatga attttctgga attgttgtag aagtaggaga
     3841 agatgttaaa aatataaaag taggagatag agttacatca gagactactt ttgcaacttg
     3901 tgagaaatgt atatattgtg aaactaaaga ttataactta tgcccaacta gaaaaggtat
     3961 tggtactcaa gtaaatggaa gttttgctga gtatgtatta tcaagagaag aaagcataca
     4021 tgtacttcct gaaaatgtat ctttattatc agcagccctt acagagccac tagcttgttg
     4081 tgttcatgca gctcttgaaa agactactat agaaagttct gatacagttt taataatagg
     4141 accaggacca attggtttat tattagctca agttgttaaa tctcaaggtg ctacagttat
     4201 aatgtctggt gttacaaaag ataaggaaag attagaaata gctaaaaatc ttggagtaga
     4261 tagaactgta aatgtaatgg aagaaaattt ggcagatgta gtgaatgaaa tgactgatgg
     4321 aaatggagta aacaaaggat ttgattgttc tgggtttatg ccagcagtta acgaagcctt
     4381 aagactgctt agaaaaaaag gtactttcgt acaagttgga atctttgcta aaaaattaaa
     4441 tgaaatggac caagaagcaa taatccaaag agagttacaa tatataggaa gtagatctca
     4501 aaagccaagt tcttggatta cagcattaga tttaatggca agtgggaaag taaatacaga
     4561 agctttagtt actagaactg ttggtttaga cgaatttaaa gaaggtatta cagctttaat
     4621 gaacggggaa gaaataaaag tagcagtaaa atcttaaaaa aacatttgta ttattttaaa
     4681 aaaaattgta aaatctactg taggataaaa aaatgtaagg tcttaaaaaa taacaggagg
     4741 aagaaagtat gagcactctt agagatcatt ctaataatat tagaaaaaac ataataaaaa
     4801 tggttgcaga agctaaaagc ggacatccag gtggttcatt atcaatagct gatatgttga
     4861 cagtattata ttttgataag atgaacgtat gtgcagaaaa tccaaaaatg gctgatagag
     4921 atagatttgt tctatcaaaa ggtcatgcag caccagcttt atatgctaca ttagcagaaa
     4981 aaggattttt cccagaagaa gaattaataa cactaagaaa atttggttct aagttacaag
     5041 gtcacccaga catgaaaaaa gtagcaggaa tcgatatgtc tactggttct cttggtcaag
     5101 ggctatctgc agcaaatggt atggcacttg caggaaaatt agataacaaa gactatacag
     5161 tatatactat attaggtgat ggagaaatcc aagaaggtca aatatgggaa gctgcaatga
     5221 cagcatcaca ttacaaatta gataatttaa ttgcatttgt tgacttaaat ggattacaaa
     5281 tagatggttc taatgaagaa gtaatgaatg taaatccagt agatgacaaa ttcaaatctt
     5341 ttggatggaa tgtaatagta ataaatggac attcttttga agaaatagga aatgcagttg
     5401 atgaagctaa aaaagttaca ggaaaaccaa ctgtaataat agctaaaaca gtaaaaggta
     5461 aaggtgtttc atttatggaa aataatgctg catggcatgg aacagctcca aatgcagaac
     5521 aagcagagca agcattgaaa gatttagaag gaggtctata ggaatgggaa tagcaactag
     5581 agaagcttac ggacaagtgt taaaagaact tgcagaaaat aaagatatag tagtattaga
     5641 tgcagattta ggaaaagcta caaaaagtat atcatttaaa gaagttgccc cagatagatt
     5701 ttttgacatg gggatagcag aaggtgacat gattggtaca gcagcaggtc ttgctacttg
     5761 tggaaaaata ccttttgcga gtacttttgc aatatttgca gcaggaagag gctatgagca
     5821 aataagaaac tctgtagcat atccaaattt aaatgtaaaa atagcagcta ctcatgcagg
     5881 tgtaacagta ggtgaagatg gtggtagcca tcaagctata gaggatatat ctctaatgag
     5941 aggtatacca aatatggttg tattaaatcc agcagatgct ttagaagcaa gacaagctat
     6001 tcttgcatct attgattata atggacctgt ttatataaga ttaggtagag ctgcaactcc
     6061 tgatgtaaac agtgaaaatt ataaatttga aataggaaaa ggaactgttt taagagaagg
     6121 ttctgacata acagtaatag ctacaggaat aatggtagca aaagccttag aagcagcaga
     6181 agaacttgct aaagaaggta taaatgtaga agttgttaat atatctacaa taaaaccatt
     6241 agatgaagca ttaataaaag aaactgctaa gaaaactgga aaagtagtta cagcagagga
     6301 gcacagtata ataggtggtt taggttcagc tgtatgtgaa gctttagcag aaactaaaga
     6361 tgtagtagtt agaagaattg gtgttaaaga tgtatttggt caatcaggta caccagctga
     6421 tttattaaaa cactatggtt taactacaga agatatagta aaaaatataa aagagttatt
     6481 atagatataa atatatctaa ataacaaatt gatttgacat aagtcaatta aagctgtctt
     6541 aaaaatagaa agaaatttct acaatgggac agctttattt ttacaatact ttatttttat
     6601 gataattata aaatagtctg aaagtaatca aaaaaattat aaaaagaggt gctatttaat
     6661 gaaaatatct attggatgtg accatggtgg ttatgaatta aaggaatata taaaggaaaa
     6721 tttaataaat aaaggtatag aagttgaaga tgtgggtaca aattcaaaag aaagtgtaga
     6781 ttttccaata tatgcaaaaa aggtaagtca aaatgttcaa agtaaaaaat ctgatttagg
     6841 catactttgt tgtggaacag gaataggtat gtctattgca gcaaataagt ttaaaggaat
     6901 cagagcagct gttgtttctg actgtttttc agctaaggca actagagaac acaataatac
     6961 aaatgtactt tgtttaggag aaagagttat tgggaaagga ttagcaatga gaattgttga
     7021 agaatggtta aattcaagtt ataatggaga aagacatgca agaagattac aaatgattga
     7081 tgatatagaa aaagaaaatt tataaatata taatttataa caagggggag cgttatgtct
     7141 attatttgtg cttcaataat gtgtgcagac caaatgaagt taaaagagga attagaagct
     7201 cttgaagaag caggtgtaaa attacttcat tgtgatgtta tggatggaat atttgttaaa
     7261 aacttggcta tgggaccaga gttattaaaa tcaataagtg aaaatacaac aataccttta
     7321 gacatacact tagccacaga aactccagat aaatatattg atatgatgtc ttatataaag
     7381 ccaaaatata tttcttttca tgtagaatca agtacaaatg taaaagctga tatacaaaag
     7441 ttaagaaatt atggtatagg gccagtgtta gcaataagcc ctcaaacaag tgtggataaa
     7501 atagaagaat atataagttt agttgagggt atcttagtaa tgacagtaaa tccagggttt
     7561 gcaggtcaaa agtttaatct ttctgtattg gataagttgg ataaacttac agatatacta
     7621 aaagattatg ataatcctcc atttatagag gttgatggaa atataaataa ggatactatt
     7681 aaacttatga atggtaaaaa agtagatata tatgttgttg gtacttcagc tttatttaat
     7741 gataagcctc caatttcata taaagataaa atagaagaac taaaagaaag tataaagtaa
     7801 ccatgtaata aaattataat atgtttctgt ttaatagtaa aaatttaggt ggaaacaaga
     7861 aattttataa tatttaataa aaattttggt tgagtaagga gaatttttta tgggagacct
     7921 atataaacta caaaatggta ctgatattag aggaatagcg tatgaaaatg attctaaaga
     7981 agttaaccta actgtagaag aagtaaaaaa aatagctaaa gcgtttcata tttggttaaa
     8041 agaaaagact aagaaagata aagtaactgt tgccatagga actgattcta ggataacagg
     8101 aagccaattt agaagtactg ttattgaaac tttaactaat gacgattgta atgtaattga
     8161 ttgtgagatt gccacaactc cagctatgtt tatgacaact attatggatg gatacaattc
     8221 tgatggggcg attatgatta cagcaagtca cttaccttat tactataatg gtttaaagtt
     8281 tttcacagaa agtggaggtt tagaaaaaac tgatataaaa gagatgttgg atatagctgt
     8341 taaaaatgat agtgtagact atgaggaagt aaataaaaaa ggtgaagtta tagtaaaaaa
     8401 tttaatagaa gattattcta atttattgat agataaaatt agaaaaggtg taaattcaag
     8461 taaaaattat gaaaagcctt tttctgggct taagatattg gttgatgcag gaaatggggc
     8521 tggtggattc tttgctgaaa aagttttaca catactggga gcagatacga caggtagtca
     8581 gtttttgaac ccagatggta tgttcccaaa tcatatacct aatcccgaaa acaaagaagc
     8641 aatggaaagc atatgtaaag ctgtgttgga taataagagc gacctaggaa taatctttga
     8701 tacagatgta gacagagctg ctattgtagg taaaaatggc aaaccaataa ataaaaatgc
     8761 tcttatagct gttatatcat caatagtatt agaagaacat ccaaaaacag ctatagtaac
     8821 agattcgatt acttctgaag gacttgctaa atttataaat gaattacaag gaagacatca
     8881 taggtttaaa agaggatata aaaatgtaat aaatgaagct ataagattga atagtgaagg
     8941 tgaagagtgc catttagcta tagaaacgtc tggacatgct gctttaaaag aaaattattt
     9001 cttagatgat ggggcatatt taatagctaa aattttaata aaagtagcaa agctatcctt
     9061 ggaaggaaag actatagaag aattgataga aaaccttgaa gaagctcaag aagagaaaga
     9121 agttagaata ggcattaata aagaagattt tagaccatat gcagaggata tattaaaaga
     9181 tttagattct catgttaaga gtattgatgg atggtctgta gcaccaaata attttgaagg
     9241 cataagagtt aattgtgatg aatcaaatgg agatggatgg tttttactta gaatatcact
     9301 acatgaacca ttattagctt taaatattga atcaaataaa attggcggag cagaacaaat
     9361 ttattctaag ttaaaacttt tcttagaaaa atatgattta aaaggtcttt agggtaaata
     9421 gaaagaatat atttatattg aagaacaaat gtaaatttgt acctaagatt acaaatatta
     9481 caaaattgta atcttaggta acagagatgt aactgattat ggaaacacta tgtgatatta
     9541 tagatacaga gagatgaaat atatgatgaa ttatagagaa atataattta aaatgtatta
     9601 atatcacacc aaaaaggtat aatcaaaaaa tatgtaaata taaaccccaa catttagaaa
     9661 aagacctcta aggaggtttt tttcttgttt aaataactta aaatatattt ttatattaat
     9721 atatcaatta actcttctaa aagtaagcat taccttaaat aaatcagcat ctataattat
     9781 ttccatgtgt gcattttcta attcaattat attttgagca attgataaac caagaccaga
     9841 gccatctgtt tttctagata tatcacccct tctaaatctt tctattaact cttctggctt
     9901 tagatttagt ttatcatttg aaatattttt aaacgttaat attatatttt cttcctttat
     9961 aaacatatct atatagaccc tagtgttagg catagagtat tttagtatat tgcaaattag
    10021 attctcaaaa actctccaag tttttttacc atctatcatg gtaaaaacag catattcagg
    10081 tgtttctatt ataaaatcta aactgctatt ttgaattttg tcttcaaatt ctacaatact
    10141 ttgaattaaa agttctctta tatcggtttt tgttatatta aattctacat ctccactact
    10201 tagcttagat gcttcaaata tgtcatctat taataatttt aatctttgtg attttttatc
    10261 taaaatattt atatagtcat ttatagtctc tctgtcaaag tcattatttt ttaacatatc
    10321 aacatagctt aatatagatg taagtggagt ttttaagtca tgagatacgt ttgtgataag
    10381 ctctgccttc atcctttcat tttttatatt agcatctaca gcagtttcaa gaccgcctgt
    10441 tatattattt atacttttgg ctaaagtaga tatttcatca tctcctataa cttctaattt
    10501 atactttaaa ttgccatttt ctatattttt tatatttttt gatatgttat ttatgtaatt
    10561 aaacttaaca gataaaaagt tgtatatata aaaatgaaaa actgtgttta caagtgtaaa
    10621 taaaattact atagctattg cctcagcata atatatatac ataaagaata tcaaaacaag
    10681 atattggata atagcttgta caactataga tattaaaaat actatagcta
//
LOCUS       DAFKMX010000135        10718 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFKMX010000135 DAFKMX010000000
VERSION     DAFKMX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.135"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(79..711)
                     /locus_tag="LZB08_002739"
     CDS             complement(79..711)
                     /locus_tag="LZB08_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBM7316229.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(783..1136)
                     /locus_tag="LZB08_002740"
     CDS             complement(783..1136)
                     /locus_tag="LZB08_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBM7316230.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKSQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1214..1417)
                     /locus_tag="LZB08_002741"
     CDS             complement(1214..1417)
                     /locus_tag="LZB08_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBM7316231.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1807..2037)
                     /locus_tag="LZB08_002742"
     CDS             complement(1807..2037)
                     /locus_tag="LZB08_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316232.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2039..2446)
                     /locus_tag="LZB08_002743"
     CDS             complement(2039..2446)
                     /locus_tag="LZB08_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7316233.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2806..3255)
                     /locus_tag="LZB08_002744"
     CDS             complement(2806..3255)
                     /locus_tag="LZB08_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316234.1"
                     /translation="MELIEWNYTYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            complement(3269..4195)
                     /locus_tag="LZB08_002745"
     CDS             complement(3269..4195)
                     /locus_tag="LZB08_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316235.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4320..4724)
                     /locus_tag="LZB08_002746"
                     /pseudo
     CDS             complement(4320..4724)
                     /locus_tag="LZB08_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4874..5494)
                     /locus_tag="LZB08_002747"
     CDS             complement(4874..5494)
                     /locus_tag="LZB08_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBM7316236.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5582..6280)
                     /locus_tag="LZB08_002748"
     CDS             complement(5582..6280)
                     /locus_tag="LZB08_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7316237.1"
                     /translation="MYKLSYVVRVSTKDLDELKRRCYEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLNTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAKRGKW
                     KETLPEIAHEINIVNLTSDDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6381..7196
                     /locus_tag="LZB08_002749"
     CDS             6381..7196
                     /locus_tag="LZB08_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBM7316238.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHKDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            7868..8635
                     /locus_tag="LZB08_002750"
     CDS             7868..8635
                     /locus_tag="LZB08_002750"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBM7316239.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEVKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(8963..9640)
                     /locus_tag="LZB08_002751"
     CDS             complement(8963..9640)
                     /locus_tag="LZB08_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316240.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(9790..10107)
                     /locus_tag="LZB08_002752"
     CDS             complement(9790..10107)
                     /locus_tag="LZB08_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316241.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10129..10467)
                     /locus_tag="LZB08_002753"
     CDS             complement(10129..10467)
                     /locus_tag="LZB08_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316242.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3579 a         1670 c         1515 g         3954 t
ORIGIN      
        1 aaataatata caaattttga aaaagctctt ataaaatgat tattaaagta aagtttaata
       61 taaataaatt ataaaatatt aaactaattt atcaatattt cctgattgaa tatgcttaat
      121 gtttgattga atttttttat atggccaatc ccaccattta atttctaata attttgaaat
      181 tgtatcatta ttaaatctct tttttataat ttttgcaggt actcctccaa caatagaata
      241 cggtggaacg tcattagtaa ctactgctct tgttccaata attgcaccat caccaatatt
      301 tacacctgac ataattatag aatcataacc tatccacaca tcatttccaa ttataatgtt
      361 acccttattg tcccaagcgt ccgttatatg gttgatatcg agtttccatt cttcataaaa
      421 gatgggaaaa gtatatgtgg attttgattt cattgagtga tttccactag tcattagaaa
      481 ttttgcatta catgctattg agcaaaactt accaattatt aatttatcat tgtttatagg
      541 gtaatgatac aatacattgt ttttctcaaa atctcttggg tcattataaa agtcattata
      601 tattgtgtaa tctccaactt ctatattttc ccttgttatt acatttttaa gatatacagt
      661 ttgataatca ttacttcttg gatatacctt atttgaactt ggaatagtca tatttaaatc
      721 tctcctttaa tatatattat gtttttgact attccactac tacaatacat ctcttcacag
      781 aatcactcct tgttaagaat cttcataaga taatggcgtc cttttttagt gttaagtcgg
      841 atattctttc ttagtacatt ctttttagcg tataactgac aaactgtcat tttcttacca
      901 atagtgtcaa tgctatcatc aaggtcattt tgtattgctt tttccttatc ccttaatgat
      961 atatcatcac gttttccctt tgggggttta tcagtaggta caagtttcca agagtaaaca
     1021 aattgtgatt taccaaatga atatacatac ttatatgcgt atcttccatc ttttctctgg
     1081 ctctctccag ttcttaaaat tctatcttta ttatctcgtc ttttttcagt tgacatgata
     1141 agaagctcct ctcaataacc gaattgagct tgatacttca tatgaatatt ataacatata
     1201 aggctcaatt tcattagata aagtcaagtg tatcaacgat tttctcaaat tgttttctct
     1261 ttatttgaat gcgattgcta ttcataatga cccagccaga atcaaggttt tcatcagcaa
     1321 gtttacgtaa ttttttctct ccaatacgaa agtattttga cgcttcttca atcgtcaagg
     1381 tatatttttc ccaaataggt acatcagtat tgttcataga atgtatcact cctttctatg
     1441 ttcgtagttc agaagtagat agatggcttt ggtaagctcc attggtattt caaccaatcc
     1501 cattcttatg ggcagaaccg tgtcatacag gtgtatcatt atactgtggt acaggttacg
     1561 gcaaaacaac cgttacaaag gttattcaca aatcactata tagtagctct cttgattaag
     1621 gtcatggcgt atagtatttg ttggctatct gtatcacaaa cgtatctatc tactttattc
     1681 agttgtcaaa gaacagtcgg cattgatttg cctataaaaa atcacaatag cttatgggct
     1741 tttatagaca aataattagt gattctccat gtaccaaata gcttgataca tggagagagg
     1801 gcgagattaa gacaaattaa atctcaaaat agcagtgatt agttttgttc ctagcattcg
     1861 ctttgtttct tcttctacat gaatataggg atttccgtat tcatcaaata aagttttctg
     1921 tgaaagacga atgatatagt gttcaaaatg tttaagtatt acgtttattg catttacatc
     1981 gccatgtaca gcaagtaaaa taattgtaaa aggtaatact tctttagtag gtttcatttc
     2041 aatgttcctg catgagttct ttcaactctt ttagtgcatt tgttttgtga taatgtatgg
     2101 tattttctgc taaatccatc aatgttgcta tgtcagcatt tgtcatgttg agaaagaatg
     2161 aaagtagaat tatatcttgt tttctttttg taagttttcc gattgcttgc gccattaata
     2221 catcttcaat tcgtatgttg tagtcgtaag aaaccataca gatgtagtta gaatcataat
     2281 catctaaaat actcaattta cttaattctg caagtgacaa agattcgaga gataccaaat
     2341 aatcatttct gcgtttattt tcgtcataaa tatctctagc atagttacgc aaaactgttt
     2401 tacagaaact gtcgaattga tgttcaattg tcttttcatc aaccataaga tttcaccctc
     2461 tttccatcgc aaggagttaa ataaccttgc ttactcctat cacggtggga gggcgttaaa
     2521 tcttcgataa atttcaaaaa aaagagaaaa aaatacacac tctgcctata gggcaaaaat
     2581 gtgtattctt taaaacgtat ttaatttata ttggttgtaa gccaccacca tattgatgta
     2641 tggaaatatg cattggctag cttttcataa aaatacaaag atgcataaga aattaataga
     2701 ggggaaaaac ttattctgtt attaaaaagt aggttaattc taataaatgc tattgcggtc
     2761 gaattaaact atatacatgg caatcaatca tgtttctatt ctttattatg aattgtgaca
     2821 gtattcttca atcttaaaac cacatttttc caatacttta cttgactctc tattgtagga
     2881 atatacatta gcatgaattt tgttaaggtt gtatttttca aaggcaattt tgcatatttc
     2941 ttttactgca tttgttgcta tccctttgcc ccaatagttc ctagcaagcc agtaacctaa
     3001 ttcagcattt ttattaatag aggtatcttc ttcaaatgtt aggctgatac ttccaatagc
     3061 cctttcttga taaaaaatag catagctaag ttgtttaaca tctggagttg ccaaacaaaa
     3121 actaataaaa tctctagcgt tttgctccct ataagggtgg gggaagttgt cacgaagatt
     3181 ctttgcaatt tctttatcat tcgcaaactc aactaaatcg gtaatataag tataattcca
     3241 ctctataagt tccatttttc ttctcctttt atgaaacagc tacaagaaat ctttctaagc
     3301 caataccact tatcaaacag cgttcttttt tatttccaag catttgataa gtccagtcaa
     3361 caaatccacc gtcaactatt tcaacggttt catttccttg tttgaccgat aatttgaaat
     3421 tgataccttt ataataatta ttatcaacat catcattatg aattgataac tctatattag
     3481 gaaaaataag tttgatagtt tcaaccattc tgtcgaaaaa tccgtcatta tctttgtatc
     3541 cgtttctttt gcgtagaata atagagaaac gaacattttc tatttcactt aatatatctt
     3601 tataatagtt taagtgcttt tctaacaaca ttttttcaca agtgtaagac cctgtatcta
     3661 taccagatga aaccatacaa tataagccaa aatgtgaata taaaccttta cctacaaaat
     3721 tttgtgctct tattacccta gcagtagtag ccatgtggat aggtatggta ttattttctt
     3781 cttttctttt taatttatca gcaataatta ttgccaacat atttgagggg tctgcaagtg
     3841 tttctgtccc tcgtagagca cttactacat tatattgatc tacggagcca aaaacgctac
     3901 agctacctaa tggagcagag ggagaaagca taatcgtctt gatatccata ttgaatgctt
     3961 ttctcaataa ttttgactcc aaagcatgaa tttcttctgg atctataccg ctagggatag
     4021 taaatctatt tagttggtat gactttagaa tatcagttgc agttaaagta tttgcttgtc
     4081 tatcaaacac ttctaataga agtgagttca aatctgattt tgatagagct gataacttat
     4141 caactatatt cttatcccca attttatttg taattctact taaaatatca ctcatagtta
     4201 tcctccatac tgttactttc aaaataatta tagaatggtt acaacaacta atctaggtca
     4261 tttgaccaaa aatgaaaaaa cactgaatat ttactattca atattttttt actttgggtt
     4321 tatttgatta aatcttgaaa atactgtaat gcaacccttt cagcttcaac ctttcggaca
     4381 ataggacttt gtgataattc ctctcttaac tcaagataca ggttgatagt agcctgcaat
     4441 gcattaacac agctttcata ttcatggtta gcaaccgtct tttctaatgc ttcaatatat
     4501 tggggtgcgt ccaattcaat ttttctaact ccacgaggta atttacctaa gagaagctgt
     4561 attaacgagg caagtactga ttgtcttaaa aaagataaaa aatcaattgt ttcaaatagt
     4621 tctctacgtc caatcttagt gcaagcgtaa tgaacccaaa tccaaaatct atcttcaatc
     4681 cactgtaagt ctggagttgg gaagtatgct tcttttttag tataacacct ctcactttct
     4741 tttattagct atcaatccaa cataattatg cataggagta ttatataatg aagaaataaa
     4801 aaaaaatagg tcacttgacc cgaaataaaa aagcattgaa taattattat tcaatacttt
     4861 tttgaaatta tacttatgtt gtaggaagat tctttaatac ctctcgctga ttaattacac
     4921 tgaatctttt gttttcataa gcaattagat gttcttgtac acattcattt atagcacgat
     4981 taatgcttct aatcggagcg tttacttcgc tagcaagctg ttttttggta agcagattta
     5041 attcacttct ataatagtgt aaggatatac acctcagaag tcgttcttta acacttaaag
     5101 atgataattc ttcaattaat tcagcattta caactaattt actagatatt tctcgaatca
     5161 gaaattttgt agcttcaaaa tctcccctta accagtttaa aaaatcatct ctatgaagtt
     5221 tatatacatt acaatctgtg aaagcgacaa tttcaacggg ttttttccca tcattaaatt
     5281 gttccatttc tccaaaaaaa ctaccacttc tataaagatg aatagttgag atgtttcctt
     5341 tcacatttaa gatgaatgct tctgcaatac cgtcaatcat aagaattaca tattcatttt
     5401 cttgctcagc aaaaagaata gtctctttct ttttatacgt ttttttaaca agcttctttt
     5461 taatatcttc attcataaaa tcaattaaat gcatattcat ctctccttac caaatctttg
     5521 attaaattat atcatctttc aattagttag gtaagatagt aaaaatatat ttacatagaa
     5581 actattccat tattattttc cagttgtcat ttttctgcaa aaacttatca ctgtcacgac
     5641 tggagatacc agtaaggaat gttaggatat caatcgctaa actttcagaa tcctttttac
     5701 ggctcaaaat gacgtaaggg tcaagcaatc ctttgttttc atcatcactg gttaaattga
     5761 caatgtttat ctcatgggct atttctggta gcgtttcttt ccatttgccg cgttttgctt
     5821 tagggtcaac tatgaccgct tgtccgccga atagtaccgc atagtaaaca agcatattgt
     5881 tactgaataa tttaccacca cccaatgaac cgataaacgc agacgctaac gcattggtaa
     5941 tagaaccctt taccccttga ctagcaaggg caggttttag atagacattc tttccagtgt
     6001 tcagattgta gccaagatag atatcctcat gttcgccaag ggtttgtgtc gctccaaaac
     6061 caagtccagc caaaaaatcc gaagtgacat attgaatata atcattgatg taacgtttgc
     6121 tgacaggaat aaattcgcca tgcaatccca acatatcccc aaagggacgc acgagcttga
     6181 ttgataagtc gtcgtaaaag tctttgactt cataacaacg cctttttagc tcgtcaagat
     6241 cctttgtaga tacacggaca acatagctaa gtttatacat agattcttta cagttaaaag
     6301 tctgttttta aaaaaatgta taaattataa atcaaaatat agatattatc aatgacccac
     6361 taggtgcaaa aagcattgat atggttaaat tatcagacgc taaaagctgg gctatccgaa
     6421 tgagtgaaga tgggtgtgct tataaatcca ttgacaatta taagcgctca ctgaaagcgt
     6481 cgtttttcat ggcaattcaa gacgactgta ttagaaagaa tccatttaat tttcagttaa
     6541 gtgatgtttt ggaagataca acagaaccaa aaatcgctct aacaccagaa caggaaatgg
     6601 gcttattatc ctttatggag agcgataaga cttattgtaa atattatgat gatgttgtta
     6661 tcctcttaga aacaggacta cgcatttctg aattatgcgg tttgacttta gaattggata
     6721 tgaaaaataa agccattatg gtagaccatc aattgttgag tgatactaag attggctatt
     6781 atgtagaagt tccaaagacc acacagggta agcgagaaat tccaatgact gaaagagctt
     6841 atcaatcatt tgaacgtatc gtggaacggc gtaagaacgc taaagcagaa ccaatcattg
     6901 ttgatggtta ccagaacttc ctatttctta aacaagatgg attaccaaaa caagcaactc
     6961 attatcaaag catgttaaga gcgttagtca agaagtataa caagacccat aaggacaagc
     7021 taccaaagat tacgccacat acctttaggc atacactctg tacaaatatg gctaacaaag
     7081 gaatgagtcc taatatattg caatatatta tggggcataa aaacattact atgacgcttg
     7141 gctattatgc acatggttca tttagttctg cgagagctga aatggaacgg ttataagcct
     7201 agtagtatga ctagagttta ctacttattt actacttttg atggcattct tatacggtta
     7261 aatgccaact tatgcaaagt atcttccaaa aagaaaatgc cgatactcct tgtagataca
     7321 ggatatatcg acatttacca acttatgaaa agataatcaa atttaatata agtttttcaa
     7381 ataaaaagat accaaattct ttttcgagaa tctatataac attacttaag cttggttggg
     7441 gtataaacaa ttgagtagct acttttgtat ggtgttcaac tttagtcaaa actcttaagt
     7501 aatgcaaatg atttagattc attaatattc aatctcttca taagtagtga aattcgagtt
     7561 atttctgcat aattatacta tgatttatag attaattcta ttagcatcca gaataatatt
     7621 gtttatatga ataaaattta aagaatattg tgaaaattat agaatgttaa aataaattta
     7681 ggtaaaaaag aagtgtttgt atattataga gtaccttttc taaaatatgt gggattgttt
     7741 taagaattag aataaaaatt atttttaaat tagtattttt ataatgtata gtatttttaa
     7801 tgtataatta aattgtgcag aaaatagaat ttaaagtcct atagaatttc tgattatgga
     7861 ggagaatatg aaaagaaaag ttcaagtaaa aaatattact attggagaag gtagacctaa
     7921 aatttgtgta ccaattattg ggaaaaataa aaaagatatt ataaaagagg ttaaagagtt
     7981 aaaagatgca tgtttggata ttatagagtg gcgagttgat ttttttgaga atgtagaaaa
     8041 tattaaagaa gttaaggaag ttctctatga attaagaagt tatatacatg atatcccttt
     8101 attattcaca tttagaagtg tagtagaagg gggagaaaaa ttaatatcaa gagattatta
     8161 tactactttg aataaagaaa tttctaatac aggtttagta gatttaatag atgtagagtt
     8221 atttatggga gatgaggtca tagatgaagt agttaatttt gctcataaaa aagaagtgaa
     8281 agtaattata tctaatcatg attttaataa aactccaaaa aaagaagaga ttgtatctcg
     8341 tttatgtaga atgcaggaat tgggcgcaga tttacctaaa atagctgtta tgccgcaaaa
     8401 tgaaaaagat gtgttagtgc tacttgaagc tacaaatgaa atgtttaaaa tttatgcaga
     8461 tagaccaata ataacaatgt caatgtcagg aatgggtgtt ataagtagat tgtgtggtga
     8521 aatatttggt tctgcattga cttttggagc tgcaaaaagt gtttcagcac cagggcaaat
     8581 atcatttaaa gagttaaatt cagtacttaa tctattacat aagagtatta attaaattaa
     8641 ttatatagaa atattagacg gtatttttga agatataaat taaatataaa aaacttttca
     8701 gatagtaaaa tttaaattgg gcaattatat ctgaaaagca actatttagt gtgtattttg
     8761 aaagtgatat agaaaatctt ataaatcaac tatggcatat ttaaaatatg ccattaaaac
     8821 ctactagaca ttagattata gatggtatac tagcatttct attatttata gatagtaagt
     8881 tttcataata actgttttat ctaaagtaga agatatattt gtattatgta tatttgtcat
     8941 tatttttttg taattatgta tattaatggt tatatatgta atataatatt atcactctat
     9001 tttttgtata ttgtacatta caacatttat atctatcatc atcaattaca gtagaatgta
     9061 tgggatactt attttcactt aaccaatatt tttgcatatt ctttgtccaa gactttggaa
     9121 acttcaatac ttttttgttt attataacat cctcatcttc aaaaatatta acttctcttt
     9181 ctatttttat ttttctgttg tatatcttat tgatagattc tttgtcgagt tttatgtccc
     9241 tatacatatc agggcaatag caacaaacat aatttctttt ttttcgacgt ttaaatttca
     9301 caactttctc tatgctaatt tctaattttt ttgtaccatc ttttataaat ttaatatctt
     9361 ctatgttctc gcttgaaaaa atttctgtca atcttaaagt atctacagtt tctctaatta
     9421 tttgcttttt ataagaagag aatccatatc caaaagaaag gtttaattta ttagggatgt
     9481 agccattttt atatttaaac atcttaattt tttccaattc agcattatct ttaaatttca
     9541 acttttcata cctaaaagtt actttgctaa aaagagtata ttctacatca atatattctt
     9601 ttgcaatctt aaatttatcg ctaccttcaa tcacaatcat agcctatctc caatcgtttt
     9661 atattaaaat aaggccatag aacatggtct aatagtactc attagcacta taagacaatg
     9721 ttaacacaaa aagacaaaaa aaggttattt atttcaaata accttttttt ttatataaaa
     9781 tataaagaat tatattttat tcttttttat gatgtctata atctgtgata tcagatttag
     9841 ttctcttgaa tttaagtctt ttaactcttc atttatatca ttttctaaat atgtttttcc
     9901 agtggaagta atatctttta taagaacatc tgcagaaata tttaatatat ttacaaattt
     9961 tatgaatgtt ttaagacttg gattcttttt accactttcg atttgagtta tataccaagg
    10021 cgatactcca accatatttg caaattcttt tgaagttaaa tttgcttttt tccgttcttc
    10081 tttaagtctt ttacctatat aattcattat tgttactatt tccctcactt atatttcttt
    10141 gtatttaatg atgcacttta ttaactctag cattgcaatt tgttcttttt cagttaattt
    10201 attaatcatt tcataacatt ctaaaggtac agagttatca acgccttctg ttgtaataat
    10261 atttgcaaat aacaattcaa gtggtatgtc taaagcagta gcaattttca ttataatatt
    10321 gatagatgta tttgtctcag ctctttcaat ctgaccaata tatgttggat gtaaacctgc
    10381 cttttctgct agaaactctt gactatatcc caattttgtt ctatagactt ttattctatc
    10441 tcctattatt tttcctatat ttgccaatta ttatccctcc actttagatt atattcatag
    10501 tttaagttgt tttatataga ctatttatac ttttttagtc taaatataga ctatatatgt
    10561 ttttatactg aaataagtgc tatttatttt tgtatagtct gtatctacaa taaacaaata
    10621 tggataaatc atatttttaa ttagagaaat attttcataa tttcatagcc atactacaat
    10681 aataaaacga attaataata tgttaattat agcatttt
//
LOCUS       DAFKMX010000136        10666 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFKMX010000136 DAFKMX010000000
VERSION     DAFKMX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.136"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            119..967
                     /locus_tag="LZB08_002754"
     CDS             119..967
                     /locus_tag="LZB08_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBM7316243.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1046..2968
                     /locus_tag="LZB08_002755"
     CDS             1046..2968
                     /locus_tag="LZB08_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBM7316244.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3050..3913
                     /gene="aroE"
                     /locus_tag="LZB08_002756"
     CDS             3050..3913
                     /gene="aroE"
                     /locus_tag="LZB08_002756"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBM7316245.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKIKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIKSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4113..5330
                     /locus_tag="LZB08_002757"
     CDS             4113..5330
                     /locus_tag="LZB08_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7316246.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5566..7431
                     /locus_tag="LZB08_002758"
     CDS             5566..7431
                     /locus_tag="LZB08_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBM7316247.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7609..8973)
                     /locus_tag="LZB08_002759"
     CDS             complement(7609..8973)
                     /locus_tag="LZB08_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316248.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9152..10495)
                     /locus_tag="LZB08_002760"
     CDS             complement(9152..10495)
                     /locus_tag="LZB08_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBM7316249.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10568..10666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         3950 a         1356 c         1834 g         3526 t
ORIGIN      
        1 agaaatcaaa aaatactttt atttttaagg taaaataata ctactagtag gataaataaa
       61 aatataaaat caaaaatact attaattgta aagaaaataa agggggcaaa tttaagctat
      121 gaataatcaa aaaaatctta atattccaat tacaattagt tcttatacat tgggaacaga
      181 agtttcattc cgtgatagag ttcgtattgc aaaagaagct gggtttgatg gtattggatt
      241 gcgtgctgaa aattatattg atgcaaaaaa tgcaggtgtt acagatgaag aaatgattgc
      301 aattcttaat gaatatgata tgaaagttac agaagtagaa tatataactc aatggggaag
      361 agaagaagat agaacggaag ctcaaagaga aaaagagcaa aatgtttatc atatggctca
      421 cttatttaat gttaaacata taaattgtgg attattagag atgataccag aagaacaaat
      481 tataactgca ttgggagaat tatgtgacag agcaggtgaa cttattatag gtcttgagtt
      541 tatgccatat agtggtgtac cagatttggc tactgcatgg cgtgttgtga aaggatgtaa
      601 tcgtgaaaat gcaatgctta tattagatac ttggcactgg gctagagcaa agcagacagc
      661 agaggacctt gcacctgttc cagcagataa gatagtatca attcaagtgt gtgatgtttt
      721 agaaactcct tatgaaaaac ttagggatga atcactacat gaccgtttgg ctccaggtga
      781 aggatatgga aacacagagg aatttgtgaa aataataaaa gaacacaata tatcaccaag
      841 agtcataggg gcagaagtaa tatctgattc tctaatatct aaagggttat ccactgctgc
      901 tgaaactgta tttaatgcaa ctaaaaaagt tttggataaa gcatggtctg aagtatctcc
      961 aaaataaact tatataatat aaaaatatac agagtattat aatataaaac ttttaaatat
     1021 aaaacttgaa aggaaggaaa accttatgaa gattagttct atgtttacac ctataagaat
     1081 tggttcaatg acagttccaa atcgttttgt agttccccct atgggaaaca attttgcaaa
     1141 cacagatggt actttaagtg aaacttctaa ggcttattat cttgagcgtg ctttaggtgg
     1201 ttttggatta ataactatag aatctagtgt tgtagacaaa aaagcaaaag gaggtcctcg
     1261 taagccttgt ttatatgatg atagcacaat agatagtttt aaaaatgtaa tagatgcttg
     1321 tcatgatgca ggttcgaagg tatctataca attacaacat gctggttctg aaggaaatga
     1381 aaaagtagca ggacatccat taaaagctgc ttctgcaata cctgcatcaa atggtcgtaa
     1441 tacaccacta gcaataacaa cagaagaaat atatgaactt atagaatctt atggagatgc
     1501 tgcattacgt gcacaaaaag ctggtgcaga tgctgttgaa gttcactgcg ctcatggata
     1561 tctggtcagt agttttatat cacaaagaac aaataagcgt gtagatgagt ttggtggatg
     1621 ttttgaaaat agaatgagat tacctcgtct tatcattgaa aatatacgta aaaaagtagg
     1681 aaattctcta gcaatattat gtcgcataaa cagtactgat gatgttccag gaggaattga
     1741 tgtacacgat agttcagtaa tagcagcata tctagaagat tgtggaatag atggacttca
     1801 tgtttcacgt agtatacata tacatgatga atatatgtgg gctcctacga ctttacatgc
     1861 tggattcagt gcagaattag ttactgaaat aaaaaaagct gttagtatac cagtaataac
     1921 agttggacgt tatacagagc cacagtttgc tgagttaatg gtaagacaag gaagatgtga
     1981 tttagtagca ttcggtagac aaagcttggc tgatccagaa atgcctaata aagctaaaaa
     2041 tggaaaacta gacgaaatga taccatgtat tgcatgctta caaggatgtg taccaaatat
     2101 gttccaaggc aaacctatag cctgtcttgc aaatcctata ttaggacatg aagcagagct
     2161 taagccagct gaaattagca aagaagttct tgttgttggt ggaggtgttg gaggtatgct
     2221 tgcagcatgg gtatgtgcta aaagaggaca taatgtaact ttagttgaaa aatctgaagt
     2281 tcttggtggg caaatgcgtt tagctgcata tcctccagga aaaggagata ttactaactt
     2341 agttagaagt tatatttcaa aatgtaatca atatggtgta aaaatatgta ctaatacaga
     2401 agctacagta gaacttataa aggagaaatc acctgatgta gttattatag ctactggagc
     2461 aactccactt gtacttccta taccaggaat caatgattca ggattaattc atgctgtaga
     2521 ccttcttgat ggtaaaaaat cttgtgggaa aaaagtactt gttgttggag gtggaatggt
     2581 tggatgtgaa gtggcagcct tccttggaga acaagaacat gaggttactg ttattgagct
     2641 tagagaagaa gttggtgcag atgtaatttc agaacaccgt aaattcctta tgaatgattt
     2701 tgaaaattat aatatacata ccattactgg tgcaaaagtt tctaaatttt atgatgatgg
     2761 tgtcgactat ttacttacag atggcacaga gcatgattta aaaggttttg attcagtagt
     2821 acttgctatg ggttctcgta attacgaccc attaagtgaa gttataaaag aagttgtaaa
     2881 agaaacttat attgttggtg atgcagtaaa agctcgtagg gcattagatg caacaaaaga
     2941 agcttttgaa gtagcattaa atatataaat aataaataaa tttttctttg taaataactg
     3001 aataggttat ttgcaaagaa aatgataata ttgggaggag attatagtta tggaaatttc
     3061 aggtagaaca ggattatttg cattaatagg tacaccggtt ggtcactcta aatcaccagt
     3121 gatgtataat tatagtttta agaaattaga tttagattat agatatttag cctttgatat
     3181 cacagtagat aagataaaag aggcactttt agcaataaaa acttttaata taaaaggagc
     3241 caatgttact atgccatgca aatctgctgt tacagaatat atggatgaac tttctccagc
     3301 agctcgtata attggagcat gtaatacaat tgtaaatgac aatggaaagt tagttggtca
     3361 tataacagat ggagtaggct atgttcgtaa tttaaaagaa aatggggtag aagttaaagg
     3421 taaaaagatt acaattatgg gtgctggtgg tgcagcaact gctatacaag tacaatgtgc
     3481 tttagatgga gcaagagaaa tatctatctt taatcctaag gatgattttt ataaaagagc
     3541 agagcaaaca gttgagaata taaaaaaaga tgtaccagaa tgtgttgtta atctatatga
     3601 tttagaagat acaaacaaac tttatgagga gataaaatca agtgatatat tgacaaatgc
     3661 aactcttatt ggtatgaagc cttatgacaa tgaaacaaat ataaaagata ctagtgtttt
     3721 aagaaaagac ttagtagtaa cagatgtcgt ttataatcct aagaaaacaa aaatgattga
     3781 agatgctgaa gctaatggat gtaaggctat tggtggatta ggaatgttat tataccaagg
     3841 agcagaggct tttaatctat atactggatt agaaatgcct gtagaagaag ttaatgaatt
     3901 atgctttaaa tagataagag taatatctta aacaatgaaa aaacatacgt atttcaatat
     3961 ttttaataat aaattacaaa ttgtcatagt gggttttgcc tcatagtcca gttaaatttg
     4021 aagctttaac tgactatact ttaaatccat tatggggatg agtttattgt acacaaaaat
     4081 gttaataaat taacaagaat gggagaaaaa ttatgaacaa taaaaaatat tatcctactg
     4141 ccatagcgtt gtatttttca tattttttac ttggtattgg aatttcaatt ttaggacaat
     4201 acaaaccaga attttcatca atgtgggggg ctaaaacatt atcagatgga acattggatg
     4261 taagtattgt tttagctgtt attgctgcac taggattagg gagacttata tcttatccat
     4321 ttgcaggacc tatttcagat aagtatggaa gaaaagtaag tggtcttata ggaaactttc
     4381 tacatgcaat tttctttgtt ggaattgtat tttcaccaaa tttttatata gcatatgtat
     4441 ttgcaataat tggaggggca gcaaattcat tcttagatac ctgtgtaact ccttcttgta
     4501 tggagatatt tgctagttta ggaacaatag caaatatgtt tactaaattt acaatagctt
     4561 tagcacaatt tttgcttcct tttataattg gatttgttgc agcaaattca atatcattta
     4621 aagttatttt tatagtaaca gcgattctta ttgtagtaga tgcctttttg atagctgtgt
     4681 taccatttcc accagctaat aatgttattg ataacaaagg caaaacagtt aaatcagaga
     4741 agatgaaatt tacaccaaca agtatagctc ttgtttgtat tggatttaca tgtacatcta
     4801 catttgtatt atggttaaat tgtaatcagg aattgggtaa attgtatggg atggctgacc
     4861 ctacaaaaat tcaatccttt tactcaatgg gagtaatatg tgcagtatta ataacttctt
     4921 tacttattaa aaaatatatt aaaccaataa gaatattagt tatatatcct ataatagctt
     4981 tacttatgct attagttgta tattttgttc aaactccaac tatttgtatg ttagggggat
     5041 ttgtaatagg ttattcagca gcaggaggtg ttcttcagtt aacaacgtca actgcaaatg
     5101 aaatgtttcc aactaataaa ggaaaaataa catctatagt tatgattgca tctagtatag
     5161 caaactatgt tattttaaat atagcaggta ttattacaaa atcaggggga gttaatggac
     5221 caaaatatgt tgtattgttt aatgtggcaa taacatttgt tggaatttta cttgcattgt
     5281 ttgtaaacat gagatatgaa aaagaaaaag tgtatgatta tgatgtctaa tattagtata
     5341 atatatgcat agagaagtct aaatctagga tagattttta ttaaaagtaa ttaaaattaa
     5401 taaggatatt atttacagtt ataagatagg gtatttacat aattaataat aaggatatta
     5461 ctagaatttc aatttctagt aatatttttt tgcgtataaa tgtaaacttg acgaaataag
     5521 tagtataatc aatataaatt acatcatgta aaggaggata atttggtgga gaatgttttt
     5581 aagcgattgc aggaatttaa tggttatgat ggatacaaag aatcatttga aatgaattac
     5641 ctatgtatat atgaaagtat accgttaaga gaacaagtag agcttgcaaa taatctagta
     5701 gatgaaattt taaatatgta taaaagtgaa tctaatgaaa tttatttatt ggaggatagt
     5761 aattctaagt ctttaatttg ttatttcgaa atttttatga aaaagataaa tactttggta
     5821 aaagaaatga ttattgatga gaaatggtta tataaattaa ctaaagaatt aatatataag
     5881 agtaaaaaag ttgagtatgt aaaactaggg ttagttttaa gtgagaaata tctaaatgta
     5941 gaaaacttaa gagaagttgt agatacattt tctaaatctg gagaatacgt attttatctg
     6001 agtaatacta ttaaaaaact tgaattttac aatacatatt tatttaattt atctaaaaag
     6061 gctacaggtt caataaaagt atttgctatt gttaatatgg aaaatttaga ttctaaaata
     6121 aattcctatc taattgaaga tggttataag gatacaaagt acgaacgatt attgatgaac
     6181 tatattattt caattgtaga tttgaatgaa tatcttgaaa aacgagattt agataaagaa
     6241 aagataaata atttagcacg tcttatatgc aattacttgc taagtgtaga atttaaatat
     6301 ataggtaata aattagaatt agtgaatcga tttttaccta ctgtagtaaa ttatgggact
     6361 aactttgaaa gtttatattc aattttccta atagctataa acgtcttaaa agatgaaaat
     6421 atagagtgca ataagattga atttgaaaaa gaaataaatg atatcttgct cagtgaaaaa
     6481 tggaaaaata tatattttga agcattaaga gatgctagtg gaaaaacaga agatattata
     6541 aaaatgtcag agatttatga tgtgaactta tcttttgatg acttgttgcc atatctgaat
     6601 agagatatta gagattttga ggtatattgg catatatcta aaaagggaac gacaagttct
     6661 agattaaaat tattaaattt ttttgaagag acgtttaaaa tagatgattt aattggaaaa
     6721 atgaaagata tagaaaaaga taaattgact caagaatatt atgatgatat gttatttttt
     6781 attgtgctta aaggaagtaa atcactatat cctgaaggaa agaatatatc tttaaaagga
     6841 atatttggaa atataaatga agtaagaaaa gaatccataa atatcttaaa aagatacaga
     6901 gagaaactat ctcttgaaga attaaaaatt gttaaagaag cgtatgaaaa agaaaaaaat
     6961 gttatattga aggatgaatt aagaagagta ctatatgaaa gtaataattt aaaaaaagaa
     7021 tttgtaaata ttgaaaaaat aaaagtagat gaacatggta aggatatcta tttgacttca
     7081 atagctgttg ctggttcaag gtttagaaat agagaatatc ttgaaaaaga attggagaaa
     7141 agtaagatat actatttaac tagagagaaa gataatctat atgatgaaaa agctattaaa
     7201 atagttggtg aaactggata tgtgataggt tatgtcccaa gaaaagaaaa ctatattttg
     7261 agcaatttat tagatggagg aaaactgctg tattgtagag taactgaata taatttatat
     7321 gaagattgta tctacgcaaa tgtgtacttg agttataaag atgtaattga aacagttgaa
     7381 aactctttaa agatggtttt agataagagt agaataaaac ttataaacta agacgtttat
     7441 atattgctta gatgatgtaa tcttaactga ttggttattt tttctggaat tatggtgtat
     7501 tttatgagca atgctaatga atataaaata aaaaaacaat tgttttgtat atatgatgta
     7561 tataaatata tttatatatt catatttaca aaacaattga aatttatgtt attttataat
     7621 tttaaattta gatggaattt taacttctgc tttatctttt attttagaag cttgtttttt
     7681 aactttattt aataattcag attcatctac aactaatact tttctttcct tcatcaacca
     7741 tttaccatca caaatagtag agtctacatt ttcactagtc atagaataaa ctatatttcc
     7801 aattggatta tgcattggta aacaatgaat tgtatctgga tttagaataa tcatatcagc
     7861 tttttttcct acttcaagac taccaatttc tttttctagt aaagcacatt ttgctccatt
     7921 tatagtagcc atttctaaga cttcttcagc tggtacgaca gttgggttta aagttcttcc
     7981 tttatgtatt agtgatgtaa gatacatgtc cctcataagg tccattctat tatttgaagg
     8041 agctccatca gtaccaattg aaacaggtat tcctttttct aacatttcag gaattgatgc
     8101 aaatcctaga accactttca tagctgaacc tgggtcatga gaaactttta catcatgtaa
     8161 tctaaataaa tcaatttcct tatgagtaag ccaaactgta tgagcagcta aaagatttgg
     8221 tcctaataca cctagtttat caaggtattc aactgttcca actccattgt tttgctttac
     8281 aaatgaaatt tcatcagcta tttcagcaat atgcatatgt atacctgtat tgtatttatc
     8341 agctagttct ttagttctaa caagtaattc ttcagttgta ttaaaaatag ttcttaatgc
     8401 aaaccatatt ttaattctct cattagatgt attattaaac ttatggaata agtctaattg
     8461 agtattgatt tcctcatcag tagttttttg ccatgcctca ggaagaccat ctcctgtatc
     8521 cataactgat ttagctaaac aggctctaag tccagtttta tcaacagcct caaccatagc
     8581 atctacatat tgaccaccag attctaaaaa tgtagtgaca ccagatttta tcaattcaac
     8641 acaacatgca gtagatgaaa tcaaggaatc ttcataattg aagctacttt cgtatggcca
     8701 aattctctct ctaagccaag ttaaaagtac aacatcatct gcaagaccac gtcctaattg
     8761 ttgagataag tgaacatgcg tattgactag ccctggcatt agtatttttc ctttagcatc
     8821 atatatctct gcatctttat ctacaatatt aatatcaaac ttaccaacct cttttataat
     8881 attattttca ataagtatgc ttccattttc atatacctct ctatctgcat tcatagtaat
     8941 tagatatgca ttttttataa gtaattgtga cataaacacc actccttttc tatatatttt
     9001 tatatctata tattatgata ataaattttc tatacaaagt taatagcaaa atcaatttac
     9061 atataaagtg attttgctat ttataaattt aaaataaata agattttatt ctatataatt
     9121 taaaatattg acctagaact atttaaaaat attaacttag aactaagttt acaactgcaa
     9181 gtaaacctat aatccaaagt gtaatactga ttttatcagt ttctttagct actacatgca
     9241 ttattatata tgaaataaat ccaaagcata aaccaatact tatactataa gttaaaggca
     9301 tcaataaaac tgtaacaaaa gcaggtacag ctattttgct atcagcaaaa tcaatctgtt
     9361 ttatgctttc aaacatgaat acaccaacca ttacaagtgc aggagctgca gcgtaagtag
     9421 gaactatacc aagtaatggt gtaaatataa gtgtagctaa gaataatgct gctgttacta
     9481 ttgaagcaag tcctgttctt gcacctgctg caataccaga agcagattct ccaaatgttg
     9541 taacagtact agttcctaag aatgagccta ttaaagtaga agaaacatct gcatataaca
     9601 ttcttcctaa atcttttatt tcaccattct catcttcaag acccatttgt ttacaacaag
     9661 taataagaaa tgctaatgaa tcaaacatgt ctataaacat gaaagagaat ataggtccaa
     9721 ttaatgagaa tttcaatgca cctacaatat ctaatttaaa agcaactggt gctatactag
     9781 gaggtgttga gattattgct gttggcaact ccacaaatcc aaatataaca gatattattg
     9841 ttgcaactac tatacttatc aatattccac ctttaacttt tttatattct agtattgcca
     9901 ttaagataag agcaaaaaga gctattaaaa ctgttggtgt aaaatctgca agtgaaacta
     9961 tagtgtctgg attagctatt ataacaccca tattttttaa accaacgaaa gcaaggaaaa
    10021 gacctatacc tgctgaagat gctactgcca attctctagg aattgcgtct gcaattgtct
    10081 ttcgaagacc tgttactgcg agtgtaaaga agacacatcc agatagaaaa actaccccaa
    10141 gacctgtttg ccatgggatt cctgcaccta taaccaaaga gtatgtgaaa aatgcattta
    10201 atcccatacc aggagctaga gcaaatggtg catttcctaa aatcgcaaat ataagtgtac
    10261 caattgctgt agctagtata gtaactgtta taagggcggc tttatccatt ccagcttcag
    10321 ataatattgc tggatttacg aaaactatgt atgccattgt taagaatgta gtaactccac
    10381 ctattacttc tgttttaagg ctagagccac gttcgctaaa tttaaacatt ttgtctaaaa
    10441 agcttaatga tgaattattt ccacctggtt gtacattgtt agtgtttggt accattttta
    10501 cctccaaaaa actttaagat ataaacaaaa aaatatatat tcatatagat agaatagaac
    10561 atatattcag agttgacatt ctattcatag aaaacccatt taaggtgagt ttgtagaaac
    10621 tattagaccg tattactaat attatatgaa cattttctat ttgttt
//
LOCUS       DAFKMX010000137        10589 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFKMX010000137 DAFKMX010000000
VERSION     DAFKMX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.137"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(131..700)
                     /locus_tag="LZB08_002761"
     CDS             complement(131..700)
                     /locus_tag="LZB08_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBM7316250.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            903..1130
                     /locus_tag="LZB08_002762"
     CDS             903..1130
                     /locus_tag="LZB08_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBM7316251.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(1349..2092)
                     /locus_tag="LZB08_002763"
     CDS             complement(1349..2092)
                     /locus_tag="LZB08_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBM7316252.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            2260..3132
                     /locus_tag="LZB08_002764"
     CDS             2260..3132
                     /locus_tag="LZB08_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7316253.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            3132..3797
                     /locus_tag="LZB08_002765"
     CDS             3132..3797
                     /locus_tag="LZB08_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBM7316254.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            3818..4675
                     /locus_tag="LZB08_002766"
     CDS             3818..4675
                     /locus_tag="LZB08_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7316255.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(4831..7377)
                     /locus_tag="LZB08_002767"
     CDS             complement(4831..7377)
                     /locus_tag="LZB08_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7316256.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNMLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(7377..8063)
                     /locus_tag="LZB08_002768"
     CDS             complement(7377..8063)
                     /locus_tag="LZB08_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316257.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(8146..9135)
                     /locus_tag="LZB08_002769"
     CDS             complement(8146..9135)
                     /locus_tag="LZB08_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316258.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(9122..9808)
                     /locus_tag="LZB08_002770"
     CDS             complement(9122..9808)
                     /locus_tag="LZB08_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316259.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(10308..>10589)
                     /locus_tag="LZB08_002771"
     CDS             complement(10308..>10589)
                     /locus_tag="LZB08_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316260.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3730 a         1748 c         1542 g         3569 t
ORIGIN      
        1 attatataat taaaaatact ctataaaagc taaaaagttt ttctattcgc tacatctaaa
       61 aataattaga tagaggtata aatgtattta gtttatttat acctctagtc ttttataaat
      121 tatatctcta ctaaattgat tttttagcac gaataagtgt gctcttggag ataccagtca
      181 tcttcacaac ctctctatat ggcttagaat ttaataaccc caatgcatat tgtatctgtt
      241 gattgctgta tttctttggc ctaccatctt taaagtcctt tttcttttta gcaaattctt
      301 ttcctaattg agtacgttct attatcatat ttctctcaag ttctgcaact gcaagtaatg
      361 tagtcaaaaa gaatttaccc attgaagtat tttctaaaag gcctatattt aaaatatgaa
      421 cacttacttc tttagcaaat aaaccttcta ttagttgtat tccttctgat gtacttctag
      481 ctagtctatc cagcttagtt gcaacaagta agtctccctt ttttagagat gatataattt
      541 tttcaaattt aggcctgttt gtagtagtgc ctgtataagt ttcctttatt attttagcat
      601 cattatattt attcaaaatt tcttgctgtt gctgatacaa actgttgttg tctgtttgat
      661 tttttgtact tacacggcaa tatccatata taataatcat cacttcctaa cataaagatt
      721 ttgtacagaa gttacccaga agcttgtttt tacttatctt tatccatttt actaaaacga
      781 tttttgggtg tcaatactgt taacttttga caccataata attcctttat aatttgtttg
      841 actatgtcaa aaattaccaa tataatgaaa ttaatgagtt tatacaaatt ggaggaaata
      901 atatgaatag tctgttaaaa gaagcaatag atgaattaaa aaacatccat tgcggggaag
      961 tttttttatt aaaagaattg ttcaaaggct atgaatggaa caggttggag atgaatatca
     1021 gattgaaatt aggaaaactt tttttgtatg aagtggtaac taatcccaat ttaaatgtag
     1081 aggtcatcaa taaaaattct gcttatcagc aaatgtataa aaaaaaatag tgacttaaat
     1141 gtatataata gtataatctt cttattgatt caaaagttaa aacttttaat acaaaacaat
     1201 cattctattt taacatttat caatatgtct aaatttatat aattaacaca tacgaatagt
     1261 attgtttaaa tcacattatt taagatagat aaatcttaac cctactccag cataaatact
     1321 aaaatagggc ttagaattat ctatattttt atcccataaa atgcttagct cttcctcttg
     1381 attgaacata tatatctggg gaaatacaat agaagcgtaa atttataaaa tttccaaagt
     1441 cagaaaactt cagttcaaac ttaggtatac caaaactttc aggtgctatt tcgtcaacgc
     1501 caaaatagat gataattcca ttatctgtaa gataaaatgc ttggtcttca ggtataacta
     1561 actcaacatt agaataatac atttctctat tttcatttat ttgagattta atgtaatcag
     1621 taattatatt aatataatca actccatctc taaaaatgtc attaagtaaa agttcttttc
     1681 cagttgtcaa atctatattg taattattca atgtattgta tttaggccca tagttattag
     1741 caaaccccat aaggtttaat atcatactca gtacataatt tttattaaaa gtaactgcgt
     1801 aactggtaga tacccaatag ctttgttttg gaagtgaatt ttgagaagct gcgttattat
     1861 attctcgttc ttcttcctgt aagccatttt taaaattatt tacagtcatt cttacagcat
     1921 tgtttatgtt atttaaaata actgggttta catacgtgga ttgatttcta atattgtata
     1981 taacaggata ctttacatca tatgaaaaat acttacttct ttcaaaatct tcattaaatt
     2041 gtacatataa tatattatat gtacaggtat ttgtagattg ataattttcc attaaaatcc
     2101 tccccctttt ataaaaaaat cagatacaat ataatatgct taaaacagta ataatgttaa
     2161 tggtaaatgt aaagaataga aagtatctaa aacagcaaaa aatgactaat tttgtggtat
     2221 aatacatagg attgagagtt attttatagg aggaaaaata tgttattatt agcagataaa
     2281 tggaaagatt atgaacttat agatatggga aatggagaaa aattagaacg ttggggaagt
     2341 tatgtactta gaagaccaga cccacaggtt gtatggccaa tggaatctga atgggcttta
     2401 tggaaaaacc ctcatggaca ttatcacaga agcaataaag gtggaggtca atgggaacac
     2461 aaaaaaagat atccagaaca atggactata aattataaaa acttaaagtt tcacattaaa
     2521 ccaactggct ttaaacatac tggattattc ccagagcaag cagctaactg ggattggatg
     2581 atagagaaaa taaagaaagc taacagacct ataaaagtgt tgaatctttt tgcatataca
     2641 ggtggagcta ctgtagcatg tgcatctgct ggtgcagaag tatgtcatgt tgatgctgca
     2701 aaaggtatgg ttaattgggc aaaagaaaat atagaattat caggtcttaa aaatcaaaca
     2761 gtaagattta tagtggatga tgttgtaaag tttgttgaaa gagaaataag aagaggtaaa
     2821 aaatatgatg caataataat ggaccctcct tcttatggaa gaggtcctaa aggagaagta
     2881 tggcaaatag aagaaaagtt atacagtttt gtagatttat gtatgggagt tttatcagat
     2941 aaccctttat ttttcttaat taattcttat acaacaggat tttctccaat agttcttgaa
     3001 aatgtattgt ctacaacaat aggtaaaaag acaaatacag gagatatata cggtggggaa
     3061 ttaggaatac cagcttccag agatggtaag atacttcctt gtggtatatt tggaagatgg
     3121 gagaaaaaat aatgataaaa gtcatatatg aagataacca tttattagtt gtagaaaaac
     3181 cagttaacat attgtctcaa ggagataata ctaatgataa agatatggta aatcttttaa
     3241 aagactatat taaaaaaaag tataacaaac ctggaaatgt atatattggt ttaatacata
     3301 gattggatag accagtaggt ggagttatgg tttttgcaaa aacatcaaaa gcagcatcta
     3361 gattgtctga gcaggtaaga agtaagactt tcaaaaaaac ttatctagct gttgttcatg
     3421 gtaagataaa atctaactca gatatactaa aagatttttt atataaaaat aaaaaaacta
     3481 atatggtaac tgtagtgaaa aaaagccata aagatgccaa agaagctgaa cttaactata
     3541 atgtattaga ttttaaagaa aatctaagtt tagttcaagt taatttaaag actggtcgtt
     3601 ctcatcaaat aagagttcaa tttgcaagca gaaaacatcc attatacgga gaccaaagat
     3661 atggcaaaga aataaataaa attggacaac aaatatctct ttggtccaat aagatagaaa
     3721 tttgccatcc tactacaaaa gaaaaaatgg aatttacatg tcatccacct gacaaatatc
     3781 catggaatat attttaaatc tcaaatgagg tgattatatg agtaaaaaat tttcaatagg
     3841 agaaatgtct aaacttcata aaataagcat acagacacta aggtactatg accaaataga
     3901 tttatttaaa cctgttcatg ttgaccaaga aagcaattac agatattact caattgacca
     3961 attttcacaa ttagatatta taaaatattt aaaatattta ggtatgcctt taaaagaaat
     4021 taagtcaaag cttgatgaaa ataatgaaga aattctaaaa ttactaacaa ataaaattaa
     4081 tatagtggat gaaaaaatta aagaattaga gcttataaag aaggtactaa gcacaaagaa
     4141 agaaactatg gaaaaaggta tgaaagtaga tgaagttggt aaaatagtga gaaaacatat
     4201 tccacgaaga agtatcttat ctatagagta tgaagaagga ttaagtagtg aagaaactat
     4261 ggaactttcc agaagacaaa tagcaaatat attagaagat aatatatctg tgttctatgg
     4321 tggagtaagt ggacttgtat ctatagataa atttttaaaa gaagataaag ttatttatga
     4381 caatagtttt gttactgttg aaagagactt atttgatata aaatcacaaa aacatataac
     4441 agaaatttca tctggtgatt ttatttgtat gacatataaa gggccttaca aagaaaatta
     4501 taccgctttt aagaaactgg ttgattatgt tgaatataaa agaataccta ttgaaaatta
     4561 tatatatgaa atacctataa tagaccctct gtctacaaac aacgaagagg aacttctaac
     4621 agaaattcaa atacaagtta aaaagagaga aattgactta agatgtctag tgtagtaatc
     4681 tagtttactt acacatctta atatagttgt ctattatcaa catatgattt ttctagatag
     4741 tgattcctaa cagtgagatt ttcaaaaaat aggaggaaat atggttaatg ctaattccct
     4801 cctattttta attaatccat atcattttaa ttaagccatg tcatcacgaa gtgcatcaat
     4861 aatattttct ttttttatct tattaatagt atacaacatt gtaataaata ctataaataa
     4921 aacactaaat atactaattc caatactaaa ccatggaatc acaaaatcaa tatcatctgt
     4981 aaccattact ttatgaatta gccatgaaga aattactgct ataggaagtc caaagaacaa
     5041 tgctctcatg ccataaaagg cacactcaaa attcatcatc ttttggaaat catgctcaga
     5101 cattcctaca gagcgaagca tggcaagttc tctacgacgt aacttaatat tagttgaaat
     5161 tgtattaaac acattggcaa tcgaaatcag agaaatcata attatgaaag tataagcaaa
     5221 cacattggca ataaaaatgt aattacggtt ctcatccatc attccataca tattgtacag
     5281 attgtactca ttcgtaactc cctcactctc aataatttgc tccatttctg caactgactg
     5341 agtaggattt ttcgattgaa agcttaaact ttcaattcct gtagaaacac caggaatctc
     5401 aaacttatct ttggctgaat aaggagccaa tactctgaag cttgctttct tcacatctga
     5461 acttctgatt cctggaggag aatcaggtgg cactactttg acaaatgaaa tattcacatt
     5521 ttgtctctca ggtatctcca acttgccatt agtttcagga gtgaggctaa cattcataga
     5581 agaactgttg aaaatatcaa taatattatt atcctttgtt accatagaaa cagcaatcag
     5641 ttttgcattt tgtccagtat attcttctgc tggtaatcca agacttttaa taatattcag
     5701 ataagttcta tcatcaagaa attgcatatc tactggtaat tcaagcattt cactcataga
     5761 atgtgaaccc tcatagttca aataatcgtc tgtaagctca cttactttgg caacacatga
     5821 ataggtcata agtttctgat acaaaccttc gtaaacacca tttgcagttt ttagcttatc
     5881 gtaaagcttc aacatttcgt tgtctttcat accttgtgta gaaaaaataa tatcctggtc
     5941 agtaacctct gacctctgat ttgacatctg ttttaggtct gctactaaag cacttgtaga
     6001 tataaataaa actatgctta aaacaagcga caatacaata cttcgataac gtttcttgtt
     6061 tcttttaaag tttttcatag caagaactcc ctcaagacca taaactcgct gtgttagttt
     6121 tgatgtcttc acagatttag attcaatttt tacctcattt gtctgacgaa tacactccat
     6181 cactggtgtg ttggcagcct ttcttgctgg tatgtaagca gaaattaaga ttgtaaccat
     6241 acttaccact gctgcaacga tgattgcagg gatagacaat gttaatgtca acggaacgtt
     6301 tacatagaga atatttgcaa agtttttagc tatgacagaa atcacaagtc caatactgac
     6361 tataccgatt gcaacaccaa ttggtatacc aatcgcacca atgcaaattc cttcaaatag
     6421 caccgaattt cgtaactgtt ttgctgtagc tccaacagat gaaagtattc caaattgatg
     6481 tgttcgctcg ttcaatgaca tgttaaatga attatatatt agaaaaattg agcctagcat
     6541 gattatggca agcacaatgc caccaaccga gtacaaaaac atattgaaca aattgttatc
     6601 agaaacacct atgaagcgca acatattgtc attgaaaatg gaagcatggt ctttagtgct
     6661 acttatgtaa gagcgaatct gatgtggatt tttcaatgtg acaaaaaggc taagactgtc
     6721 agtcttgact tgtttatctt ctttggttat caaggtatat cctggtgcag aagattcttc
     6781 aaatacaggc tttctacaga tacccacaac tgtataagtt ttcttatcct gtggcacaaa
     6841 aacttcactc ccagcagtgt atgggtcact ctgaccaagc ttttcatttt cactcattcg
     6901 tttccctaca tccaaagaaa tagtatcacc tactgcaaat ttgacaccac catctgtcgc
     6961 aactttactt gaaacaagaa tctctctact attttcaggt aatcttccag aaattaaagt
     7021 tataggcaaa gtatcaaaag cttctttgct aaatccagca ataaaaagat atggtttatc
     7081 tggatttctg ttaccttcaa gtgttgcata gccaatattt tcaaatgttg cagtatttgc
     7141 aacttcatca ttgactcttt gttcctgtac aaaagaagaa tccacatcca aaaactggac
     7201 atgccaatca ccatatttta taatagctcc atttgtcata tagttcatca aagaaactcc
     7261 aaaggtagta actgctgtaa tcatagcagc tgacagaaca actcctataa ttgttacaag
     7321 agttcgagtg cgattctttt tcaatccttg cagagcaact ttgttaaaaa tgttcattaa
     7381 cgattcacct tcctctcatc acgcactact tttccatctg aaatacagat aattctatct
     7441 gcctgtaaag caatgttttc atcatgtgtg acaataatga gagtttggtg atatttacta
     7501 tggctctctt tcagtaactg gataatctct tgtccatttt tactgtctaa gctacctgta
     7561 ggctcatcag caagcataac tgctggtgca ttcatcaatg cacgaccaat ggcaacacgt
     7621 tgctgttggc caccagaaag ttgatttggt aaatgtgttt ttcgttcttt taaaccaagt
     7681 aattctaaca agtcatttaa ccgttcttga ttgaccttac gcttatccat aagtataggc
     7741 aatgtgatat tttctactac atttaatgtg ggtatcagat tgtgaaactg ataaatcaat
     7801 ccaacttgtc gcctacgaaa aatagcaagt ttttcattgc tttggccata tacatcttgc
     7861 ccctccagat atatctttcc acttgttggt acgtctactc ctgcaatgga atgtagtagt
     7921 gtagatttac cagaaccaga agaaccaatg attgcagtaa actctccttt ttcaatagta
     7981 agtgagacat ggtcaagtgc agtaacctta ttttcattct taccataaac cttacataaa
     8041 tcttcaatct ttaaaaacgc cattatataa gtctcctttc atattttata aatatatcat
     8101 aggactttca acttacattt aagtgacatt taaatgagag attcgtcact ttggaaaacg
     8161 aataacaaat attgcaccac cttgtgggtg attttgtgca gtaattgttc ctccttgtct
     8221 cataataatc gtcttacaaa gtgccaatcc tattccataa ccagctgtgc ttgaattttt
     8281 cccacgataa aatctgttaa atagacatgg taagtcttct tttttaaagc ctgctccgtt
     8341 gtcatgaata gtaagttgag taaacaaaaa gttgtcctca caaatgatat caatttttcc
     8401 attatcaccc acactctcca tgcagttttt aaatatattt tgaattgctt ctgaaagcca
     8461 atttaaatca cccaaaatcc ttactccttt tggtatatca atatgtaaag tgatattgtg
     8521 cagttccata ggaatcaaaa gttgatgaag tgcagaacta atcaaattat tcacatcaat
     8581 ttgttctttt tgaaagacta caatacctgc atctaaacgt gataatttta aaagggaagt
     8641 aatcagccag tccattcgta taagtaattc ctctgtttct cgtatcaact cttttctctc
     8701 atcttcatca gaagtattct ctaacagtga caaaataaga gttactgatg taagaggagt
     8761 gcgaagctgg tgggctatgt cagctagtga gtcagcaagg tgttcttttt cttttttcag
     8821 tgcgtaattt tgctctctaa tacgtaacgt cattttagtt atttcacttt gtagaataga
     8881 aagctcaccc tcatctgatt cagcaatata cagatggtta gcattatgaa gcacaagatt
     8941 aatttgttct gaaatctgtg caatactttt gtatcgattt ttagtaaaca caaaaaatgc
     9001 tattccaaag gtagtggcag aaacaataga aagtattcca gtcataatac tgattgcaaa
     9061 tcccagggct acagtgagaa tagtaattaa taagaataaa atagcaaatt ttcgaaactc
     9121 tctatttcga aacataactt ccccccaatc tataccctgt cccacgaaca gtcaaaatta
     9181 tttgtggact tgaagggtca tcttctatct tctctcgcaa gcgtttgatg tacacagtca
     9241 atgtgttgtc attaacaaac tcacctgccg catcccacaa ttcatcaagt aatcttaccc
     9301 tcgtaataat acttttagga ttgctaataa acaccaataa caaacggtat tctaaagctg
     9361 aaagaaaaac ctcattgcca ttctttttca caacaccggt tgctgtatct acataaagcc
     9421 cacaaatttc aaaagttgac ccaaaacgcc cactttttcg tagtgctgtt ctaattcgcg
     9481 caatcagttc acgtggacga aaaggtttag taatataatc atctgcaccc atattcagtc
     9541 cagtaacaac acttgattca tcaccggaag ctgtcaaaaa gataacagga atgtcttgct
     9601 tttctttgat ttctgtgcaa atcgtaaacc catttccatc aggtaaagaa atatcaataa
     9661 gtgccaaatc aaatttattc ttagcaatct ctacaagtgc ttcacttctt gtggatacat
     9721 gggttactat aaatccttct gaattaagca aaagaataag atttttggca attgctttat
     9781 catcctcaac taaaaatatc cgtttcattt actttcatcc tcctttattt tattagtttt
     9841 atatattcca gcagagcaat agtacactac tattttactt atcattacac aattttcagt
     9901 agtttctttt gttcttatat aatccatgca taatacctcc attgatttat tatatttctt
     9961 ataacataaa tacacaagat aagacaaaaa acataatgta agtgcaagtt ttttaactca
    10021 ctaacttata aacatacact cctactaaca ctactttctt gatgacacat cttacaagct
    10081 tatattcata catcaatgaa aaattaaatt tatccattta ttaaaataaa aggcaatagc
    10141 ttctatagct aatgcccttt atcttaaaat gatttaaata ctatacaatt tttataaatg
    10201 gctataaata aactcgtata gaatataaaa tttatctaca gtcttttgtt atacaaaatt
    10261 tataacctct tatagaacaa atttaccact ttgcaaactt atcactttta ctcagctata
    10321 ttataaactt ttaatttatc tttgtttaaa tgtttcttct taacttctac taaagctttt
    10381 agcgtatcca aagatgtcat tatttcagta gaaaattcaa tggctgttct tctcatctta
    10441 aatccatctc ttgtagagtc atttccttta gttggtgtat ttattactat atcaacttgt
    10501 ttatttctta tagcatctaa tatgtttggt ctaggctctt caattctatt tactatatca
    10561 gcctctattc cattttctct taaactctt
//
LOCUS       DAFKMX010000138        10505 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFKMX010000138 DAFKMX010000000
VERSION     DAFKMX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.138"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..898)
                     /locus_tag="LZB08_002772"
     CDS             complement(<1..898)
                     /locus_tag="LZB08_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBM7316261.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSRPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVL"
     gene            complement(1174..3828)
                     /gene="adhE"
                     /locus_tag="LZB08_002773"
                     /gene_synonym="adhC"
     CDS             complement(1174..3828)
                     /gene="adhE"
                     /locus_tag="LZB08_002773"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBM7316262.1"
                     /translation="MAHKEQIIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     SIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKKFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            4332..4532
                     /locus_tag="LZB08_002774"
     CDS             4332..4532
                     /locus_tag="LZB08_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316263.1"
                     /translation="MKKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(4739..5329)
                     /locus_tag="LZB08_002775"
     CDS             complement(4739..5329)
                     /locus_tag="LZB08_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBM7316264.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(5332..6207)
                     /gene="dpsA"
                     /locus_tag="LZB08_002776"
     CDS             complement(5332..6207)
                     /gene="dpsA"
                     /locus_tag="LZB08_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBM7316265.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(6283..7674)
                     /locus_tag="LZB08_002777"
     CDS             complement(6283..7674)
                     /locus_tag="LZB08_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBM7316266.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(7678..8814)
                     /locus_tag="LZB08_002778"
     CDS             complement(7678..8814)
                     /locus_tag="LZB08_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBM7316267.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRVP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(8846..9391)
                     /locus_tag="LZB08_002779"
     CDS             complement(8846..9391)
                     /locus_tag="LZB08_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBM7316268.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            9715..10485
                     /locus_tag="LZB08_002780"
     CDS             9715..10485
                     /locus_tag="LZB08_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316269.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAIKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         3543 a         1716 c         1228 g         4018 t
ORIGIN      
        1 ttaatacaat agataacact gcatatacag ctctgttgat tatttgtttt gaattactaa
       61 atacaacagt ttctggaatt gaaaaaatta aattccagtc ttttatataa ttattttcac
      121 tattcccatt atccatagtg gcatatccta aatatcttct ttcttccaac atatttaata
      181 cagttgcacc tgttttattt tcttgcaatt ctttttttaa tctatttact tcttgaatat
      241 cattatcctg ttctaacaat ttataaatat tttttgatag gctatcccct tgtggatgaa
      301 ggataattgt accatcattt tttacaacat aagatatact attattttca taaaaagata
      361 tattaataag tttcatcaga gtattgatat tgtaagtaca atataaaact ccaactactt
      421 tatcattatt taataatggt actgaaatag caatcttctt tatagactta tttacttcat
      481 caacatatgg ccttgaaaca tacttcttac ctttaattga gtttttgaaa aattctctat
      541 ctttcactga aaacttatca cctttggtat tttcagcatt accatttata tcaactattg
      601 ccatagtgta aaatttttga ttttccattt catctaaaag atttttcatt ttttcctcat
      661 taggtatatc tggatttgaa gcataatagt tagaaaaaat atttaaaaca tctaaatctt
      721 tttcaatttg tctttgtgct agttgagagc cttgtactgc aacttctttt atatgtattt
      781 ttgcctctga cataagaaga tgttttgtat cttctatata gatatacccc attgtcaaaa
      841 ataatgatgt taaaaaaaga attgtaatta atgtaaaact tttatttctc cctgtcattc
      901 gactcctccc ctaattagtc aatacttcat atagataata tatccttttt ataccaattc
      961 atcatttata ttgtatataa ctattcttaa atgttacaca taaaatcaac ataataacca
     1021 taaaaacatt cttttattat tataataaca ctatttaatt gaattttaaa ttaaagaata
     1081 aaaactatcc attttctcaa acaaaaaaac tcttatacaa taaaactttg ttttatgtat
     1141 aagagttttt attttatcta ttttatagca aacttatctt ccataatatg aatttaaata
     1201 catatctttt atttcattca taagtggata tcttggattt gctcctgtac attggtcatc
     1261 aaatgcttga attaccattt catctattga atccataaac ttagattctt caactccaaa
     1321 cttttttata gatttaggaa taccaacttt aagttttaaa tcttcaatag ctaccaataa
     1381 actttggaat ttctcttcat cagaatttcc ttttaatcct aaaaatgaag ctatttcagc
     1441 atatctcttt aatgcttctg gatatttata ttgagagaat gctcccattt tctttggtgc
     1501 atctgaagaa ttaaatttca taacttctgt tatcaaaaga gcatttgcaa caccatgtgg
     1561 tacatggtgg aaagcaccta atttatgagc catagagtga caaacaccca aaaatgcatt
     1621 tgcaaaagcc atacctgcca ttgtagaagc atttgccatt ttttctctag cttctgggtc
     1681 ttttgcacca ttatcatatg cacgtggaag atattcaaat atactcttaa tagcttgtaa
     1741 cgctaatcca tttgtataat cggttgcaag catagataca tatgcttcta atgcatgagt
     1801 taaagcatct ataccagaag ccgatgttaa gctttttggc atattcatca tcatatcaga
     1861 atcaactata gccatatttg gcatcagttc ataatctgct aatggatatt ttactcctgt
     1921 atcttggtca gttataacag caaatggtgt tacttctgaa ccagtacctg ctgatgttgg
     1981 tatagctaca aaattagctt tttcacccat tttagggaat gtatataccc tttttcttat
     2041 atccataaat ctcatagcta ggtcttggaa atctacatct ggatgctcat acaatgtcca
     2101 cattatcttt cctgcatcca ttgcacttcc tccacctata gatattatta catctggatt
     2161 aaattctctc atagctttag cgcctatctt agcacattcc aaagttgggt ctggttctac
     2221 ttcaaagaat gtagtgtgct taatattcat ttcatctaat aaatcagtaa ctgctttagt
     2281 atatccatta ttgtaaagga atgtatctgt tacaataaaa gctctcttct tattcattac
     2341 atctttaagt tcttttaatg ctactcctaa acaacctttt ttgaaataaa ctttttctgg
     2401 tgctctaaac caaagcatat tttctctcct ctcagctact gttttaatat taagtaaatg
     2461 cttaactcct acattttcag atactgaatt tccaccccaa gacccacatc ctaaagtaag
     2521 agaaggagct agtttaaaat tatataagtc ccctatacca ccttgagaag aaggagtatt
     2581 aattaaaatt ctacaagttt tcatggctga agtaaactta tcaagttttt ctctttgatt
     2641 tacatcatca atatataatg aagatgtatg cccaaatcca ccatcttcta ctaatttttc
     2701 tgcttttcta actgcatcat caaaatcact tgccttgtac attgcaagta ctggtgaaag
     2761 tttctcatga gcaaaagctt cttctatttc aactgattct acttcaccta ttaaaacctt
     2821 agtatctact gggacttcaa agcctgcaag ttttgctata gtacaagctg tttgcccaac
     2881 tattttagaa tttaatcctc catttactaa aattatattt cttatttttt ctgtttcatc
     2941 tttatctaat aaataagcac cacgctcttt aaattctttt ttgacttcat tgtatatact
     3001 ttctaatact ataaccgatt gttcagaagc acatatcata ccattgtcaa aagtcttaga
     3061 tacaagaatt gagtttacag cagtttttat atctgcacta tcatcaatta ttgctggagt
     3121 attacctgca ccaactccaa tagctggttt tccagatgaa tatgctgatt taaccattcc
     3181 aggacctcct gtagcaaggg tcaaatccac ttctttcata acagttgtag ttaattctaa
     3241 agatggctca tctatccatc ctattatacc ttcaggagct ccagctaaaa cagcagcctc
     3301 aagaacaact tttgcagcct ctatagtaga attttttgct cttggatgtg gagagaaaat
     3361 tattccattt ctagttttta aagctaaaag tgctttaaag atagcagtag aagttggatt
     3421 agttgtaggt acaactgctg caataacacc tattggttcg gcaattttag tcataccaaa
     3481 tgcttcgtct ttctctataa caccacaagt tttagtttct ttatatgcat tatatatata
     3541 ctctgaggca taatgattct ttataacctt atcttcagca attcccatac cagtttcttt
     3601 ttgtgccatt atagctagag gaactctttg cttattagct gccaaagatg ctgctaaaaa
     3661 aattttatct acctgttctt gactatatgt agcaaatatt ttttgagctt ctctgatttt
     3721 tgctagtttt ttaactaagc tttcaacgtt atttacagta tctaaaccaa ctgacataac
     3781 ttcattttct tttttatttt ctattattat ttgttcttta tgagccattt taacaacctc
     3841 ctgagatagt tttttattta ttatgttaat ttattaacat tatagcattt actttttcta
     3901 ttttcaagtt atttttttaa cattttattt tttatctaat ataacatcat ttctactaat
     3961 aatatctagc tttacaaata cttaatttaa ctagatatca ctttattttt tacgcaaatc
     4021 actaatgaaa tcatctaaaa acttaaggat aacgttattt ttttaacgtt tatatttttg
     4081 tattttttac aaataaaact ttataaaaag aatttttaat atgatactct atcaagttta
     4141 aaaataaaaa tacttgaaaa tgaaaataaa aacttaatta acaagttaaa gtaaatatag
     4201 ttaaatgtac aaacatttac aacatatagt ataattaaaa aaatatatat tcatgatttt
     4261 tatatactat gaaataaaat atatttaata ttatatagat aaatttgaag tttataagaa
     4321 ggaggataag catgaaaaaa ttaaaaacaa ttagcgtttt ttctttgatt ataagtgtaa
     4381 ttttaacgat tggaggtatt ggaatagtaa cttattatgt agataatttg tttattagag
     4441 gactatcggt atttgtttta attatgtcct cttcttttgt atcaacaaca gttagattga
     4501 tatttgaaga gtcaaaacga tataaattct aatttaaaaa ttggattaag taaataattt
     4561 taatatacca aaaattacgg agattagctc aaaatagact ttttagtcca tgaggcattt
     4621 ttttgtatga aattaaatat attttcatca tttcaacaag aaaaagaggc ttctctctat
     4681 tttgagacag cctctttgct agtattttat ctaatacata atattataag aaccaattct
     4741 attgatgtgg actttttata acaggctgta gctgtttgtt ctctagagca agttctatag
     4801 ttggaattat tagctcagta tgtgcaatca tagaattagg ctttgctttg caattatctt
     4861 gaccaaaagg tacaaaatat atatttttag aattaaggag aatgccaata tttttgaaat
     4921 taaagcttag agcatcattc gttgatattg atattactag tggtttatca ttacgaagat
     4981 gaccttttgc tgccattaat actggtgaat ctgtaatacc atttgctaat tttgcagcag
     5041 tatttccagt acaaggagct attattataa tatcagcaat tcctttaggt ccaaatggct
     5101 ctgcctcttc aatcgtaact ataggtttat ttcctgtaag ttcatatgct tttttcataa
     5161 actcttcaga atttccaaat ctacaatcga tattttgtga tacatctgaa aatatagtgt
     5221 gtacattagc tccttcttta accaaattct ctaactctat aaatatttta tcataagtac
     5281 aaaacgaacc agtaaatcct actccaattg taagtccttc aagtctcatt tctaatccct
     5341 tctttcattt ataatatttt ctatagcgtt aacaagaatt tcaccagatg tctttggtga
     5401 atatttacca ggaagcccaa ggcaaagagt cgcattaatc cctgcttctt taacaacaga
     5461 ataatctact cctccaggag aagaagctat atctataata gttacttctt gtgatacttt
     5521 ttctaacctt tctttcgtta aaatacactc tggtatggtg ttaaaaatat aatcaaattg
     5581 taaaagtttt tcatcaagtt gacttaaaga aatactggaa tatccaaatg cattagccgt
     5641 acttcttgca tcattatttc ttgcgccaac acatacattt gcatccaaag cgtataattt
     5701 ttttgcaagt atttgtgcac atcttccata acctaatact aaacagttac tttgatgtat
     5761 attgattata cttcttttaa tagcttctgc tatagcccct tcagctgtag caactgcatt
     5821 taaaatagca acatcattca ttttcataaa atcataaatc tgaatagagt gttgctcgca
     5881 aaatttacaa actttagttg gtatgttacc tccaaataat atttgattag gagataaatt
     5941 aattagcaag ttatcaatag tagcatcaag acaatcatcc gtattttcaa tattaacttg
     6001 attctttgta aatggaattg gacataaaat aatggaactt ttggatagtg cttcagctag
     6061 tgaagttgat tttccaacga ttccatttat aatttcattt gatatcccat aagtgactac
     6121 agaataccct ttattaatta gtttttttac catgtacact tgtcgcaggt ctcccccaat
     6181 aacagttata tcgtattgat ttgacatatt gtaagcctcc tttactatat acaatatgtt
     6241 tttatcaata cattggttca ctaaaattac taaatattat acttataaca tttttaataa
     6301 tgctaatcta tctatctttt cagaagaggt atatggaaat gaatttaaaa agataatttt
     6361 ctttgggatt tcatttttca ttagattatc tttcatttta atcaacaaat ctttcttcgt
     6421 aattttgtct atagttatta caaataatac aatttgattt cctcttatag tgtctgagta
     6481 cggcaataca attgcatctt caatttgagg aattttttta acttcatttt caatcttagt
     6541 agaactaact ttaaatcctc caatatttat aatgtcatca cttcttcctt caaatattaa
     6601 gtatccatca ttatcataat atccaatatc attgacagaa taaggtttgg ttgcattata
     6661 tactgcatat tctgtattca caaaaatttt tccttctcta atatatatat ctattcctgg
     6721 aaatggtctg cctacactta gaggtttttc aattagctca tcatagcata aatatgtaat
     6781 ataatttaat tcacttgccc catagtataa aataatttca gactttggca tatatttttt
     6841 tagatttctt gcagtatctt caaataataa ttgagaccct gtaaaaatac ttacaacttt
     6901 gagtactggt tcttttaagt gtttaaccaa aagttgaagt tttgtaggta taaggtatat
     6961 gttagttaca ttatattgtt ttattgtcct tatccatgat ttacaattaa gacctgaact
     7021 tattacaata ctacctcctt catataatac tgacattata gaatttagat tccctgtaaa
     7081 actaagtgtt ccattaacaa acaagattga ttccccagag atattaaaaa taccattttg
     7141 tataggaaaa aatccagccc aactctcata tgttctatat aatacctttg gaacacttgt
     7201 agaacctgaa gataaagctc ccatacaaat acttttatct aaataattta taagcttctt
     7261 atattgatac atatatatat taaatttagg attaaaaata gaaaaattaa ctttttttgc
     7321 tacttcatta ttgttatcat catataaatc aataacgtcc atagattcat cagatataat
     7381 gattgaaata ttatttttta ataaaatatc atctaatact tttttagaaa gattataatg
     7441 acaaataata ggaatatttt ttgaataatt aatagcaaaa aaactaatca actgaaacat
     7501 tatattttta gaatatatta aaattgggat attttcacct aaagaaattt gttttcctag
     7561 ttcctcacta taactcaata tattttcata agtatatttt tctccatcta taattaaaaa
     7621 atttttattt ttatgcctct ctttttttat tcttaaaagt tcataatagt tcataatcta
     7681 caccctttca agcagtaaag ctgaacctag accaccagct gcagctatag agcaaattcc
     7741 atatctaccc ttagtctttt ctaatgcttt taaaagatgt aatgcaataa ttgcacctga
     7801 agcaccatat ggatgtccat acgctaaagc tccaccaaat atattatatc tatctattaa
     7861 ctctggatac tttctttgaa acaacacatc tataactgca aatgcttcat taaattcaac
     7921 agcagataca tctaaataat taagactttc tatctctaaa agtttatcca ttgcttgtat
     7981 cgctgatgtt ggacttaagg ttgcatctgt tcctattgtg catgtattaa taatttttgc
     8041 tttaggcttc ttttttacat gttctaaata cttcttagaa catattataa taaatgaagc
     8101 tccatcatta ataagacatg aatttgctgc atttgtaata gtttcttttc ctaaaattga
     8161 tggcacccta tctaataacc tttgactcat cttatcacgt ataccttcat cataggtact
     8221 attatttata ggagatataa tatcctcaag tattttttct tctcttgctt cttttgctct
     8281 tttatgactt tcctttaccc aaaaatccaa gtctgctttc tcaatttgat atagctctgc
     8341 tactctttcc gccccttcga gcatactatt ttggctatta tcatcaggag aaaattgagc
     8401 aactgtataa tttggattat ttgtattata tcgcttatca ttcttatgat atgtgcgcat
     8461 tggttgtaga gaactacttt caaaaccacc tgcaataatt aaatcacact gaccagattt
     8521 aacctttgaa aaagctatgt ctatactcat catagcagaa gcacactgca tatccacagt
     8581 aaaagcaggt acttcatttg acactcctgc tgttaaagtc attaatctag ctatattccc
     8641 tcctgtccca actgcatttc cacatataat ctcatcaatt ttatcaattt catacttttt
     8701 aattaaatct tttaaaacac ttgctcctag taattcaggt tgaacaaatt gaaaaattcc
     8761 attttttaga cctatatgac ttctcaatcc tcctaatata aaaacttcct ccatacatta
     8821 cacccttcct tatattatag atttatcata gatttcttct gagtacattg ttcagtttag
     8881 tgcctaaaaa agatgcaata accgacttta ttgtatctcc aattaaaaat ggtactactg
     8941 ctgcaaaaag tgctgcttct actgtcattt gattaaaata gcacataaat actgttccca
     9001 taaaatatat aataggcatt ccaacaaaaa ttgttattag tatatatctc ttaatatcat
     9061 tatttcgtcc ttttaataaa ctaataatta gtgctgcaaa caaaaaccca aaataaaaac
     9121 cacctgttgg gcttaacaac tttccaatac cagatgtacc tcctccaaat actggaatcc
     9181 caattaaacc tatcattata tatacaccaa ctgtactaaa tgcttgtctt ggtgttaaaa
     9241 tcaaagctat taaatttatc attattgttt gtgctgttac tcctgctgga gtaaatggca
     9301 atggtatata tatgtaagat gatatacaca ataatgctac acatattgac attttagtta
     9361 aatcttgaat atttacttta gtatttttca tttttataat tcccttcata tgtcttattt
     9421 catttagttt atatgtacat atacaaagct tctattaaag ctttgtttta gtataaaata
     9481 aagacgtttt tttgtcaact atcataagta tttaggttta catttaattt ataagtaaac
     9541 tttatactgt tactttatat tttaatagtc atttataaaa ctctgcttaa aaacagctct
     9601 atttagacta tacttgtaaa ttataatatg atatattttt atataagatt ttattttata
     9661 taaaaaatag taacaaatag tttatacaca agatatttaa aaggaagtga tgatatgaaa
     9721 gttaatgaaa ataatataga tgaagagtta gatgaactta gtaggaaagt ttacttagag
     9781 tccccagatt tttggataag tatttctgac aaaataatag ataaagtatt tgatgaaatg
     9841 ttattatttt ttgcaattaa atataactat ttcaatatag tatgctttgc tattgaaaac
     9901 aattatctgg atttaaacat gccttcaaaa aacaaaaaat acactaacat aatggagcat
     9961 ttgttagata catcaaaaca gaatgaagat aaaaaaattt ataattatct tctaagctta
    10021 aataaaggta ataacctaga tgaaagacca attttagatg aattaaactg taatattagt
    10081 agtgatactt ttgaaaaaaa agatatatat cttccaaagt acttatgtcc aagttgtaat
    10141 tctaatatat ttgatacagg ttataaagta tcaaatgaca taacatataa attttcttct
    10201 aaagaagctg aacctattga aatgtgtagt gaagtttctt ctatcagatg ttgtaattgt
    10261 gatgaattat tagaagatat tacacttgat aaactatata acctatgcaa aattcaaaac
    10321 tgtaataatt gtggttctaa tttaactaag gtaggaatta ttgcaaagaa aaaaatgaac
    10381 tttgatgaga atactgaaaa atttaaagat gtctcaacat cttactgttg tggaaattgt
    10441 gactctgaaa taaacttatc tcaaagagaa cattttaaga tttaaatatt tataggctaa
    10501 agtaa
//
LOCUS       DAFKMX010000139        10389 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFKMX010000139 DAFKMX010000000
VERSION     DAFKMX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.139"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            34..258
                     /locus_tag="LZB08_002781"
     CDS             34..258
                     /locus_tag="LZB08_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316270.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            311..835
                     /locus_tag="LZB08_002782"
     CDS             311..835
                     /locus_tag="LZB08_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316271.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(940..1086)
                     /locus_tag="LZB08_002783"
     CDS             complement(940..1086)
                     /locus_tag="LZB08_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316272.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1225..2151)
                     /locus_tag="LZB08_002784"
     CDS             complement(1225..2151)
                     /locus_tag="LZB08_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBM7316273.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2341..4212
                     /locus_tag="LZB08_002785"
     CDS             2341..4212
                     /locus_tag="LZB08_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBM7316274.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYVSLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4304..5239)
                     /locus_tag="LZB08_002786"
     CDS             complement(4304..5239)
                     /locus_tag="LZB08_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBM7316275.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            5759..6271
                     /locus_tag="LZB08_002787"
     CDS             5759..6271
                     /locus_tag="LZB08_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBM7316276.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(6426..7487)
                     /locus_tag="LZB08_002788"
     CDS             complement(6426..7487)
                     /locus_tag="LZB08_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBM7316277.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(7611..8504)
                     /locus_tag="LZB08_002789"
     CDS             complement(7611..8504)
                     /locus_tag="LZB08_002789"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBM7316278.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(8514..9995)
                     /locus_tag="LZB08_002790"
     CDS             complement(8514..9995)
                     /locus_tag="LZB08_002790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBM7316279.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(10103..10334)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3903 a         1349 c         1489 g         3648 t
ORIGIN      
        1 aaaatatcaa ttatataagg agtagataaa aatatgataa taaaacttac taaagacaat
       61 tttaatttta gatttaaaca atatgaaggt atgttttttc atgctgaagt agttgaaggt
      121 atggaatcca ttagatataa aataactaag gataaagaag gtacagaatt aaaagaattt
      181 ggaagtacac atataaaaga taatatgata tatgtacctc aggtagaagc atacatagat
      241 ttgaacagta taaaataaag atgtaaaatt ttaattaaaa ataaagatta tgtttgaggg
      301 ggaatccaat atgagtaagc ctatagatat aacaataaga cctataagaa gagaagatgc
      361 acaagctttt aatgaaatta gaagaacata cagtgttatg agaaatactt tggcattaat
      421 gagtgacaga gtcgataaaa ctgaagctat gttagcttct ttgggagaaa atgattatat
      481 gtttgtagct gaaactaata ataatggaga aaaagatgta ataggttttg ctgggttaca
      541 tgtaaattct tctccacgat tgagacatag cgcagaaata ggaataagtg ttgcagagaa
      601 ttatcaggga agaggagtag gtaagaagct tatggaacaa cttttagata tagcagataa
      661 ttggattatg ttaactagag taggattaga agtaattgtg gataatgaaa agggattaaa
      721 tttatataaa aagctaggtt ttgaaataga gggaactaaa aaatatgctg taatcagaga
      781 tggaaaattt gaagatgttt atattatggg aagatataat aaaaatttaa tttaaagaag
      841 ctaatagaga agtaaaatag agtgtcttta ataaaaaatt tacattaaag ataatctatt
      901 ttacttctct attgtgttaa aaaacttatt tttaaatgtt tacaaaaaca ttctatatac
      961 aaataaaatt attagcaata tcaacgctat tttaaaagct aaactagcaa cagttttaat
     1021 gatttttaca gaaacaccta tagcaactat agcaaatact attttaaaga gtaaatccat
     1081 atccatattt aagcctccta ttctataata catatagata ttattgacaa atatctagga
     1141 aaaaattcca tcaatatact acaataaaat gtttatattt gtaatttatt gaatatgtta
     1201 ttatataata cataaaaaaa taaatcaaga taagagtaaa atacttataa aaggtataga
     1261 tataactgaa aatagtgttg taaaaaacac acattgtgaa gctagtgtta tattggagtc
     1321 atattgatta gccattatag cagtatttgc agcagcaggc attgaagaaa ttactactgg
     1381 tatagctaaa atcatcttat catttatcca tccttttaga ataaaataca caatcacagg
     1441 aagtaccaat aatcttataa aagtgactat atataatttt ttgttgacaa aacaatcgag
     1501 agctgaagaa ttagcaagta agcttccaat gataatcatg gatattggtg tagttatact
     1561 tcccaacatc tcaagtggct cgtttataaa ttggggtaac ctaagtcctg ttacaaataa
     1621 aaaaagtcca ataacaatag ctattgtagc agggcttata agtgatttta ttgaaaaacc
     1681 tctacttgta ctgcctttag ataaaagata aattcctaaa gtgaaagaaa ataagttaaa
     1741 aggcaaattg aatattgcag tatagaatat tgcttcatga cctaaaactg cttcaattac
     1801 aggataaccc ataaaagcaa catttgaaaa aacaactata tactgatata ttcccaaatc
     1861 ttttttatca ttacatttta aaatgtattt caataaaaat gcaattataa ttgaaactat
     1921 atacataatc aaagatgtaa ataataacaa tagtattttt tgtatcattt tactatcaaa
     1981 gttaatttgc atggagctaa ttatcattga tggtaaaaaa actgtcattg tcaaagttga
     2041 aagcttagat gtgcagtggt catctaataa gttgaatttt ctgacaaaat atccaactaa
     2101 aataattata aataaaactg caacttggat aaaaatattg ctgaaattca taaaatgtcc
     2161 cccctatata tacattatat accaattaca taataaaatc taatgattat aaaatttaaa
     2221 atttaatgtt acaatatagt gacaaaaggt aaaaattcaa tattatagca ttaattgtag
     2281 attataatgt ataataatgt ttgagagaat atattttata aaatgggtag gtagcgaata
     2341 atgaaaaata caaaattttc tataaaaaaa ataaaaaata tatctaatga cccgaaagtc
     2401 gtacatacta agggtgccct ccttgaaaag atgggtaaga tggaaagcgc aatagagttg
     2461 tataagagtg ctgcattgga taattacgta aaatctcaat atactttagg aaatatatat
     2521 gaaagtaaaa aacaatttaa agaagcagaa aagtggtatt caatggcata taaaagtgga
     2581 agtgaagatg ctgcttttga tttaggtaat atgtactata agttagatgg ctatgaatat
     2641 gctatatatt ggtatgaaaa gatagcaaat ttaggttatt tacaagcaca aaataattta
     2701 ggtgtatgcc attttaaaat gaaagatttt gtaaggtgtg aaaaatggtt aaaaacagca
     2761 gcagacaaga atttaggtaa agcttgtttt aatttagggg tattatatac ttttttggag
     2821 aaagatgatg aagcatatga atactataaa aaaggttctg tgcttttaga tgatgatgca
     2881 aaatataatt tagcaatatt aaatggaaaa aagaaagata ataaatcaac agtaagttta
     2941 tataaacagt tatacaagtc aggtcatatg aaaggttgct ttaacttggg tatgattatg
     3001 gaaataaatg ataatttaga agatgcagag aagtattata aaaagagtgc tgatagagat
     3061 gatgtgaagt ctgagtatag gcttgcatat gtatatgata gaaaagaaga gctgggagat
     3121 gcaatttatt attatgagaa agcaatagaa aaaaatcata cactttctaa gtatagactt
     3181 gctaatttat ataatagaga aaatgagata gagaaggcaa aaacttatta taaaatggct
     3241 gctgaagatg atataacaga ggcaaaaaat aatctggcag gtatatattt tgaagaaaaa
     3301 gattatgaaa atgctattaa atattatgag gatgctatag ctgttggatg caaaagttcc
     3361 cttgaaaatc taggggattt atattatcag aatcaagata tagaaaaagc gatttcgtat
     3421 tattcgagga ttccaaataa tgtatcttgt caaatcaaat taggaaatat ctatgaagat
     3481 ttaaataata ttgaagaagc aataagttgg tataagaaag catctgaaaa tggagatacg
     3541 aggtctagtt atagattggg gtgtatatat gagagcttag gaaatacaaa aaatgctaga
     3601 aagtattttg aaatggcaag tagcaaaaac catatgaatg ctcgtataca tctaggtaga
     3661 atttatttta gagaaggtaa attggaagag tctaaaatga tgtttgatac acctgccaat
     3721 gaaaataatg tgtatgctca acatatggta ggattaattt atgatatgtt ctataaagat
     3781 tatgtaaatt ctaaattttg gtatgaaaag gctagagcac aaggttgtgt tgaatcaata
     3841 tataatttag gtcaaattta tttaaaatta aatgatgacg cagaagctga gaaatattat
     3901 aaagaaggta ttaaatatgg aagtaagaag tgtgagtata tgcttgcagg tctttattat
     3961 aaaaagagcc tagatatgta cgtttcttta gcaaatgaag agtacgataa ttgtgaagag
     4021 atagtagaag gctttcctaa tttaaatata gactttgatg aagtattaat tcctccattt
     4081 aagttgcaag aagaggtaat agatgaagaa gagtatgtac caatttatat actaaatata
     4141 aaagaaagtt tagattcaat gctagaaggg cttgaaactg aaatgattat agatgaagaa
     4201 catgatagtt aaaaagtatt ataagattat atagatttaa atatataaaa gccattagga
     4261 taaatgtaaa tcaattaatt ctaatggctt ttttattaat attttataat agcttatttc
     4321 ttgacatgaa aaatgtaatc actatacata ttatagaggt aataagaagt attatccaaa
     4381 atccgtttgg gttattagaa aatggcatgt tgatgacatt cataccaaag aatcctgata
     4441 ttattgtagg tattgagaaa attagtgtaa ctactgttaa aatttgcata acattattct
     4501 gattattact tataattgca ctgaaagcat ccataattct actcaatata tctccatata
     4561 ttttagccat atcaagagcc tgtctatttt caactataac atcttctaat aggtcttcat
     4621 catctggata cttctttata ccagcagttc taaccatttt gtttaaaaca agttcattag
     4681 attttagtga ggtagagaag tataccaaag atttttctaa ttccaataat tgaaccaatt
     4741 ctttgttttt cattgatttg taaatttctc gctctattat tattgtcatt ttatttattt
     4801 ttcttaagta atgcaagtaa taagctgcat ttttgtgaag aatttgaagc aaaaagcgag
     4861 tctttttaaa tgtaaaaaaa tctttaatat gaccaactat aaagtcattt aaaatcttag
     4921 tttgtgcatc acaaactgtt acaatagctt cttcagttag tataatacct aagggtattg
     4981 tagtataatg agaagaatta gagtcacttt cgtctactgg tatatctata agtataagag
     5041 tatgattgtc ttcaacatct attcttgagc gttcttcttc atctagtgca gattctatgc
     5101 tttctacatc tatatttaat aaattagaaa tttcgttgat ttcactatga ttaggttcaa
     5161 caagatttat ccaacagcca tcttcaaaaa aactaagttt ctctaatttt gaatctatag
     5221 ttttgtaata tcttatcatg gcaacacatc ctttattcag ttttatataa atctcatcat
     5281 ttaatagtat ctattaatta actaatttat ataactgaat atatgtatta gttttgattt
     5341 tagatattat taagtgatga aaatgtaaaa aattctctcc gcaacggact agtatccatt
     5401 gcccatcact ctccttattt attattttat atatagctat ttatgaaatg cgtattttaa
     5461 gcactcttta atataatacc ttttccaggt atataaatca agttttgatt tgattaaata
     5521 atatttttac cataatttac acataaagta tataagttat atttaatttg agtaaagaat
     5581 atatatattc tgagtgaaat tattaaatat attaaaaaat aagagagtat atatatattt
     5641 acttatttgc aataatatat ttaaaaaagt attttatttt caaaactaga cttcatttat
     5701 aatttatcac gaaaataaaa ggaatataaa tataagagaa tgagaggtta ttagagatat
     5761 gagtgatttt aaagaaattt tggcagaaga gattaaaaat aatccatttg gattgatagg
     5821 taaagattgg acacttataa cagcagaaaa tgaaggtaaa gtgaatacaa tgactgctag
     5881 ctggggaggg cttggagtta tgtggggaaa agatgttgct tttgtagtaa taagaccaca
     5941 gcgttatact aaggaattta tagataatac agataaattt tctcttacat tttttgatga
     6001 agattttcgt aaagagcttt catattgtgg aaaagtatct ggtagagaag aggataagat
     6061 atcacagatt ggatttaata tagaacactt aaatgataca ccttatttta aagaatctaa
     6121 aatggctatt atatgcaaaa aaatgtatag tcagaaactt gaacctcaat gttttattgc
     6181 tgaaggtata gatggaagat ggtatccaca aaaagattat catactttat atatagctga
     6241 aataaccaat gtacttgtaa aagaagatta gttataaagg ttaatactat ttctaattaa
     6301 attaaaattt ccataaaagg aaattgacac aaaaaagcta aggacataaa tatgtttata
     6361 aagattaata aataacgtat ttaaactcct tagcttgata aaatcttaat ttagttttct
     6421 ttaaattata attcagaaat caattcattg ataacatctt caacagtact taatttatca
     6481 attttatagg catcatttcc acaaaataaa agtgaatttt caacatctcc tttaactgca
     6541 ttgattagag cttgagaaat acagtaagga gtttcttttg gattacaagg aatcaaacaa
     6601 ttatagcact tctttatttc tggacgattt attttaactt cttctataaa tttgtttctt
     6661 atggcgcgtc caggaagacc tacaggactt ttaactatat gaacatcttc ttcactagca
     6721 tttatgtatg ccattttaaa gttctcatgg gcatcacatt cgtaagtagc aacaaatctt
     6781 gtaccaactt gtacaccact tgcacccata tctatatatt ttttaacatc actactagaa
     6841 gttataccac cagcagcaac tactggtatg tttctaccaa atttttctcc ataagtattt
     6901 gcaaccttta aaatttcaac aaattcctta tcatagtcta tagaatctat gttatcaagt
     6961 tcctcgttag agtatccaag gtgaccacca gcttttggac cttcaacaac tattaagtca
     7021 gcagtagttt tctcttttct atcccacatt tttaaaatta cattagcagc ttttgcagtt
     7081 gaaactatgg gtgctatttt tacattactt ccctttacta atgatggtag cttattaggt
     7141 aatccagctc cagatattat aaggtctaca ccagctttaa cagcttcctt tacgtatgtt
     7201 tcatattctt tcatagctac cataaaatta ataccaataa taccaccatt tgagatttct
     7261 ttggccttag aaatatgttt ttttatagct cttaaatttg catttatagt attagtttca
     7321 aaatcttcct catcatatcc aatttgaact ccagaaatta caccaacacc accaagctta
     7381 gcaactgcac cagctagtga tgacctagat ataccaacgc ccattccacc ttgaattata
     7441 ggaacttttg caactaaatc tctaattatt aactctttta tgataatccc tcctcaatgt
     7501 aaacatgttt aaagaactag attataatac ttaatattat tacctagtcc taattattat
     7561 actacaaatt ataataaaag agtagttata ttttgtatag aagacgataa ttatattata
     7621 tttaaaacag ctccattaga atcgcaacat aactctttta tttcccaatc agattttagt
     7681 ttttgtaggt aatttttcat attatccaca aaactatcat cattttcttt tattaaagac
     7741 attatagtag gtccagctcc acttaaaaat actccaatac tatttaattg ttcagatttt
     7801 tcaacaatat cattgtagtt ctcaattaga gttcctctat aatcttgatg caatttgtct
     7861 tgacaagcta ctttaagtag gtcaaagttt ttgtttagta aggcagatat tagtaaagca
     7921 actctactaa cattgaatac cccatcagaa tatggtattt cctttggtaa aacacctcta
     7981 gctttttctg ttgataactt aaaattaggt atcattgcac aaaatttaag ttcttcagga
     8041 atttttataa tatcataatg tatgttctca ttatcagtaa cagatacaat catatttcct
     8101 aaaatagcag gagcaacatt atctgggtga ccttctattt ctgaagctat atttagtaat
     8161 tgatttttat ctaaattagc acctgataga gcatttgcag ctataactcc agctactata
     8221 caagaggcac tactgccaag acctctacat ataggtactt cactttggat ttttattttg
     8281 ataccagcag gtattttttc tggtttaact ctatcaaaaa agtacttcat agatgtataa
     8341 actaagttgt tttcgttttt gtaagcatct tcacaacctt ctatttcaag accactttca
     8401 atttcttcaa cataaaattt attgtatata tttaacgcaa ttccaagaca atcaaatcct
     8461 ggacctatat tagcacttgt tgcaggaact ataatctcta acataataat cacctatacc
     8521 tttaagaatt tttttatagc tagttttaat tcatcttttt cataaatatc tttatgtagt
     8581 atttctgcat ttttaagatt tttaataggt tttggaatat ctattttaga aatacttgat
     8641 aatctatcaa ttatagcaaa gtcatcaagg tctttaaaat cacaatctat agactttaga
     8701 acatcttcag aaaacttgaa tggacttgca gtacttacaa taacagtttt tgtgttatca
     8761 gaagtttctt ttttatattt ttcataacaa ttgtatgcaa cagcagtatg agtatctatc
     8821 aaataattat aatttttaaa cacattgttt atcgtgcttt taacttcttc ttcatttgaa
     8881 tattctccat agaatgaggc taaatttttt tccatatcat cacttatttt gtagactcct
     8941 ttatcactaa ggtctgataa aagcttattt acaatatcag tatttctatt tgatatttca
     9001 aacagtaatc gttctaaatt acttgaaatt aatatatcca tagaaggaga agtagttaat
     9061 ttcaatgttc tgtttttatc atatacacca gttttaaaga aatcataaag aacattatta
     9121 tcatttgatg cacatatcaa tttgtttatt ggaagaccca tttgtttggc ataatatcca
     9181 gctagaatat taccaaaatt accagttggg acaacaaaat ttattttatc atttaaattt
     9241 atctcactat tgctaactaa tttcatatat gaataaaaat agtatgcaac ttgtggaaga
     9301 agtcttccta tattaattga atttgcagaa gataatatat aattattatc caatagtttt
     9361 tgattaaatt ctttatcaga aaaaatcttc tttacacctg attgtgcatc atcaaaattt
     9421 ccttttatac caactacata tgtatttttt ccagtttgag ttttcatttg aagtttttgt
     9481 actgggctta caccatcttc tgggaagaat acaattatct ttattttatc tatatcttta
     9541 aatccctcaa gtgcagcttt gcctgtatct ccagatgtag cagtaagaat cacaacatct
     9601 ttctctaaat tatctttctt tatagatgtt ttaagaagat gaggcattat agtaagtgcc
     9661 atatctttaa atgctaaagt tggaccgtga tatagttcta agaaataagt atcatttact
     9721 ttatttaatg gagctatact actacaatca aacttttcat catatgcatt ttctatacaa
     9781 tttttaattt catcttcagt aaaatcacat aaaaatttac taagtacaaa aaatgctatc
     9841 tgagaatacg ttaaatttac taaatttata agttcatttt ttacattggg aaattcactt
     9901 ggaacataaa gaccaccatc acttgatatt ccttttatta ttgcttgtga tgcagttact
     9961 ttttcttcgg tgcctctcgt acttgaataa tacatttttt ataatcctcc atatatttta
    10021 aatttttaat tagctaatta aattagatac agaatatttt aaaaactgaa tttaggtaat
    10081 aaaaaaagac cctcatccct aaaaaaggga cgagaatctg tttcccgcgg ttccacccaa
    10141 attgatttta aaaaatccac ttgatacttt aacgcaagta ataaaaacgt ctattcctac
    10201 taaaatcatt aagataactt cggatagaag ctcctaggta gttttcaatc cttctaatta
    10261 gggaaacatt tcagctagta gtttccttct ctggtaatcg attcagattt actcttccta
    10321 atcaatgcct ttcaatattc ttaatattat taattattct agtttataaa acagacaaag
    10381 tcaagtatt
//
LOCUS       DAFKMX010000140        10368 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFKMX010000140 DAFKMX010000000
VERSION     DAFKMX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.140"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            199..1623
                     /locus_tag="LZB08_002791"
     CDS             199..1623
                     /locus_tag="LZB08_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBM7316280.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1658..3787
                     /locus_tag="LZB08_002792"
     CDS             1658..3787
                     /locus_tag="LZB08_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBM7316281.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYNKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNIVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3817..4239
                     /locus_tag="LZB08_002793"
     CDS             3817..4239
                     /locus_tag="LZB08_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7316282.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4258..5409
                     /locus_tag="LZB08_002794"
     CDS             4258..5409
                     /locus_tag="LZB08_002794"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBM7316283.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSNKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5515..5616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5799..6371
                     /locus_tag="LZB08_002795"
     CDS             5799..6371
                     /locus_tag="LZB08_002795"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBM7316284.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6435..7085
                     /gene="fsa"
                     /locus_tag="LZB08_002796"
     CDS             6435..7085
                     /gene="fsa"
                     /locus_tag="LZB08_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBM7316285.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7237..10161
                     /locus_tag="LZB08_002797"
     CDS             7237..10161
                     /locus_tag="LZB08_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316286.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4253 a         1009 c         1945 g         3161 t
ORIGIN      
        1 gtattattta caaaatcata tactccaaaa tactacataa ataaaattaa acaaaaacta
       61 aaatgacaaa tgtcattatc taaagtgaca tagaaacggt ttcttaaagt tgttactata
      121 aaatataaac tctataataa aagtataaca aaagggaatc atatcgatat agaatataat
      181 tcaagaggag gtaaaattat gagtagttct aatcaatcga caataaagac atctatacag
      241 cgagtaggga aatttttaag tggtatggta atgccaaata taggagcttt tatagcatgg
      301 ggtcttataa cagcattatt tataccaaca ggatggatgc ctaatgaaaa attgagtact
      361 ataggtgacc caatgataaa atacctttta ccactattga tagcttatac tggtggtaaa
      421 gcaatagcag gacaaagagg tggcgtaata ggtgcagcag cagctatggg agtaattgtt
      481 ggtgcagata taccaatgtt tatcggagca atgataatgg gaccatttgc aggatgggta
      541 attaagaagt ttgataaatt tgtagatgga aagataccaa caggatttga aatgttagta
      601 aataactttt caataggtat aataggtatg cttcttgcaa tactaggatt ttatgcaatt
      661 ggaccagcaa tagtagcagg aacagctctt atagaatctg gagttcaatt tatagttagc
      721 aaaagtttat tgcctctagt ttcagtattt atagaacctg gaaaagtatt attcttaaac
      781 aacgcaataa atcatggtat attagggcct ataggtatag ctgaggcaaa agaagcaggt
      841 aaatctataa tgttcttact agaaagtaac cctggaccag gtttaggagt tctattagca
      901 tattggatgt ttagtagagg aagtgtaaaa caatcagcac caggagctgt aataatacat
      961 ttctttggtg gtatccatga aatttacttc ccatatattt taatgaatcc agttttaata
     1021 ttagctacaa tggcaggtgg agcagcagga atattgactt tttcaatatt aggagctggg
     1081 cttgtagcag caccatcacc aggaagtata tttgcactta tggcactagc tccaaaggga
     1141 ggattacttc cagtattagc aggagttgca gtggcaacag ttgtatcatt cttagtggca
     1201 gcaccatttg ttaagagagc ttcagcaaat caaagtgaag aagacagtac ttctttagaa
     1261 gaagctaaag caaaaatgag tgatatgaaa tctgcaagta agaactctga gaaaaatatt
     1321 gaggaaaaac agcttgaagt aaatgagatt aagaagattg tatttgcatg tgatgcagga
     1381 atgggttcga gtgcaatggg agcttccaga tttaaaaata gaataaaaaa cttagactta
     1441 aatatagaaa ttacaaattc atctgttgat aatcttccag atgatacaca aatagtagta
     1501 actcacaata cattagttga aagagttgct aagaataatt cgagtgttga gatagtttca
     1561 ataaacaatt ttttaaatga cccaaattta gatacattat ttaaaagatt agaatctaaa
     1621 taaaaataaa aagtaccact aattaggagt ggataatttg aaaaaaaaga agattacatc
     1681 taggcaaaaa aagataatac taatgatagt agaaaattca aagaaaaaca ttccaataac
     1741 tatttctgag attgcaggga ctttagagtt aagttcaaga actgtactta gagatatgtc
     1801 tggaattgaa aaatggtttg atgaaaatga ttttaacttt gtaaaaaaac caggagtggg
     1861 attgatttta gaggaaaata tagaaaatca aaactttata attgagttgt tagaagaaga
     1921 aaaaatagaa aaagaataca acaaagaaga aagaaattta attatattat caaagctact
     1981 agtttctaac gagccagtaa aatcctatta ttttgcaaaa atattaaagg tatctgaagg
     2041 ggtacttaat aatgattttg ccttagcaag taaatggttg gaaagatttg atattgagct
     2101 tgttagaaaa ccagggcttg gtgtctactt aaaaggtcaa gaaaaaaact ttagagaggc
     2161 atatgtaaat cttatatacg attcattcaa tgaaaaagaa atactagata tggtcagaaa
     2221 tataagtgaa aatatacaaa cagataaagc aatagaaatt ttaagtgaaa atagactttt
     2281 aaatttaatg gacaggtgca taataagaaa agttgaagct acattaacaa agaagctttc
     2341 agatttagat gtaaatttag ctgatagtgc atatataggg cttgtagttc atatttcatt
     2401 agccttacag aggataaaaa atggtgaaaa tataactatg gataaagagt ttctaaaaga
     2461 attatctata acagaagagt ttaaattagc taaagaaatc gtaaaaggta tggaaacaga
     2521 ttttaacatg gatataccag tagatgaggt tggatatata actatgcata taagaggtgc
     2581 aaagcaaagg tcatcttcaa accataaggc tttaaactta gatgatatag aaataatgga
     2641 aattacaaat aagatgatag atttggcgga agatgaattt aaaataagtt taaaaaatga
     2701 cgagaggtta tttaaagacc tagcaaatca cttagggcct tctataaata ggttgaatat
     2761 gggattagaa ataagaaatc ctcttttaga tgaaataaaa agcaagtatt catatgccta
     2821 tgatggagtt gaaaagattt ctagaattat aaaagataaa ctaaatataa attcaatacc
     2881 tgaatcagaa ataggatata ttgcaatgca ctttgcatca gccatagaaa aaaatcttat
     2941 gatgaatact aatataaata ttgttgtggc atgtcccact ggaataggta cttcaagatt
     3001 cttatcaact aagattgaaa ataaatttcc taatttaaat atacttgaaa ccatatcagc
     3061 tataaacata gatgaagagt atttaaagga aaaagatgtt gatttaatag tgtctacagt
     3121 tgagttaaat acaagtttaa attatatatg tgtaggtcca tttatgagtt tagatgatga
     3181 acagataatc aaggaaaaaa taaaatctat agctcaaaat aaattaatca atttaaatgc
     3241 gaaaaatgat actaagagta agaataaagt atatgaacag ataacagaaa gtatgaatat
     3301 aggaaaagat attcttcaat ttttagagga aattagattt gaaaaatttg aaagtaaaga
     3361 tttaagtgag cttattgaag actcatcaaa aatatttgca aaatctagtg aagatataat
     3421 ctctataaaa gaatcgttaa aagaaagatt aaaaatttca attccatata tagaagaatc
     3481 aaaaattcta ttgttacatt gtatgagtga aagaatagat attatgaagt tatcaataat
     3541 aaaactagag aatagaatcg tgctagattc aaatgaagaa atcgacaata tagtttttat
     3601 gttacttcct aaaaattcac caagttatca aagacaaata atgagtgaaa taagtggttc
     3661 attaatagac aatataattt ttactaataa gataaataaa ttctctatag aagagatgac
     3721 attagaggta aaagatatag tttttaattt ttatacaaac agattaaaag catttataga
     3781 taaataaaat taatattaaa aaggaaggtt gatactatga gtaaagtatt gaatgagaat
     3841 aatatatttt tagggttaga tagtgtttca aaggaagaag ctataacttt agcaggaaga
     3901 aaattagttg aaaatggata tgtaaaagaa gaatatatac cagcaatgtt agaaagagaa
     3961 aaagtgatga ctacatatat gggaatggga gttgctattc cacatggtgt aaatgaagca
     4021 aaaaaagaaa tattatcttc tggaatagtt atattacaat tccctaatgg tatagatttt
     4081 gatggagaaa aagcatattt attaatagga atagctggtg ttggagatga acatttagaa
     4141 atactatcta atatagcagt agttttagat gatgacttaa cagaaagact aaaaaattca
     4201 aatgacaaac aagcttttat ggaagctttt gccaactaat cttaggaggg atttaaaatg
     4261 aaaaaggcaa ttcagtttgg agcaggaaat attggaagag gttttattgg aggcttgtta
     4321 gttaagtcgg gttatcatgt agtatttgct gatgtaaatg aggatatatt aaactcaata
     4381 aacaaagata aaaagtatac tatacacata agagatgtag aatgtataga tgaagtaatt
     4441 gataatataa gtgcggtttc atctataaaa gaagaaataa tagatgagat tgtacaggct
     4501 gaaataataa ctacagcagt tggaccttta gttttgacta aaatagcctc tacaattgca
     4561 aaaggtataa aggctagaaa agaaaaggga ttaacaagta atctaaatat aatagcatgt
     4621 gaaaatgcaa tatacgcaag ttcttcttta aaagaagagg tacttaagta tttaaataag
     4681 gaagaagtag aatatttaga gatgtatgtt ggttttccaa actgttcagt tgatagaata
     4741 gttcctcctg gtaaaaacga aaatcctctt gatgtaacag ttgaaaattt ttatgagtgg
     4801 aatgttgaaa aacaaggatt taaaggggaa ataccaacta tagttggaat gaatttagct
     4861 gataatctta tggcttatat agaacgtaaa ttgtttacac tgaatacagg tcatgctata
     4921 acagcttata taggatattt aaaaggatat aagactatag aagagagtat aaaagataaa
     4981 tttatctgtg atatagtcaa aagtgcaatg gtagaaagtg gagaaggatt gattaaaaaa
     5041 tacaattttg actcagaagt gcactataaa tatattgata aaattttaaa tagatttaaa
     5101 aatccatatc ttaatgatga tgtattaaga gtcggaaggg aaccattaag aaaactaagt
     5161 aataaagata gattgattaa acctttaatg acagcaaaat catatggatt atcagttgat
     5221 aatttgattc ttggaatagg tgcagcgctt cattataata atagtgaaga tactcaaagt
     5281 gtagaattac aagagttgat aaagtctatt ggggtaaaaa aagccgttgc taaaatagct
     5341 aatataagca atgacgaaga acttttaaat aatatcgaaa aaagttatat atttatgaaa
     5401 aatttataaa tttacttgaa aaaaagtagt tctgataaag taactataat atattattct
     5461 acacagtttg aattatttgt tgaataatag agaattataa tatataatta aaaggaataa
     5521 attaatatat ttacctgtat atgtttgata atatggttca aatgtttcta ctaagctacc
     5581 attaatagct tgactacagg agtgaagaag atatagttta tacttatttt aaattatata
     5641 ttaagttaat tactttgaat tttaagctcc tgtctatgat atagacagga gcttttttag
     5701 tattttaata taaaatactt acaagaatag atagcaaaat tagaacaaat ataatttttt
     5761 tattaatata attttagaaa gggctaggtg gaagttcaat gaaggcttta caagaaaaga
     5821 tattgagaga aggcagtgtt tcaggaaatg atatactaaa agttgatagt tttttaaacc
     5881 atcaaattga tgtagctttt ttaaatgaaa taggaagaga atttaaagag agatttaaag
     5941 gagaaaaggt ggataagata tttacaatag aggcttcagg aatagctata gcatcaattg
     6001 tttcacagta ttttgataat gcacctgtag tatttgcaaa aaaatcagaa tctaaaaatt
     6061 tggataaaga tgtatatgaa actaatgtat attcatttac taaggcaaga gagtattcag
     6121 ttaaagtttc taaaaagtac ataaacaaag gcgaaaatat attgatagtg gatgattttt
     6181 tggcaaatgg aagagctgct cttggactta aagatttaat tgagcaagca gaagctaact
     6241 tagttggagt aggaattgtc attgaaaaag gatttcaagc tggaggagca cttttaaagg
     6301 ctaatgatgt aagattagag tctttggcag tagttgaatc tatagataat ggaactgtaa
     6361 aatttaggta atttatgata ttatatactt gtgtttagca catattttaa ctatgtaatt
     6421 atggaggatt ttttatgaga ttttttatag acacagccaa tattgaagaa ataaaagaag
     6481 caaatgattt aggtgtaatc tgtggtgtaa ctactaatcc gtctctaata gctaaagaag
     6541 gtagagattt tatagaggta gttaaagaaa taagtgaaat agtagatgga ccaataagtg
     6601 cagaagtaat aagtttagaa cataaaggta tgatagagga agcagaaaaa ttatctaaga
     6661 tacataaaaa tatagttata aagataccta tgacagcaga aggtctaaaa gcggtgaaag
     6721 tactatcaag taaaggtata aagactaatg taacgcttat attctctgct ggacaagctc
     6781 ttttagctgc tagagctgga gctacatatg taagtccttt tgttggtaga ttggatgata
     6841 tatcacaaaa tggtcttgat ttaattgaag aaatcgttga tatatttagt gtaaatagta
     6901 ttgaggctca aataatagtt gctagtgtta gaaatccaat acatgtttta caagctgcta
     6961 gaatgggagc agatatagca acagttcctc ttaaagttat aaaccaaatg attaagcatc
     7021 ctttgacaga caaaggaata gatagcttta tgaaggattg ggaaggtgct tctttaaaat
     7081 tataattata aactacgctt tataagtata ttaaagaggt tattatggaa attctaagta
     7141 ataaggctag gctattttgc ctagtcttat tttggtgtat aatatattga aacatattat
     7201 tgaaatagat taatttttta agtgaggagg cgaaaaatgg ataaaatatt aagagagata
     7261 caaagagaag ataaaaaaaa tccatatact gaccaagaac ttgctgaaat gttaaatgta
     7321 gctaggtctg aagtgatatc agttaggaaa aaaaataata ttttagattc gagagaaaga
     7381 agaaaaaaaa tattaataaa agatatatca aaaattgtaa gtgaaaatcc tcaaatgtct
     7441 gaaagaaaaa taactgaaat attaatagaa aatggatata atatatcgag aagtgcagtt
     7501 tcaaaattat taaaagagga aaatttaaat cagtcaaaga ttgggattaa ggataaagac
     7561 ccagcatcat caggaaagta tgtgaaggtg agtaaagttg tagaagaaga tggatttgaa
     7621 gaattgatag gtataaaggg aagtttgaaa gaaaaggtaa atctagctaa gtcagcaata
     7681 atgtatcctc ctaatggcct tcatactata atatatggag aaacaggagt aggtaaaagt
     7741 gaattagcta catgtatgta taaatatgct ataaaaaaca acattaaaga agagaacagc
     7801 ccatttatag tgtttaactg tgcagactat gcagaaaatc ctaatctatt gatagctcaa
     7861 ctctttgggg tagtaaaagg tgcatataca ggagcagatg caaatagaga aggtcttgta
     7921 gaacaagcta ataatggaat attgttttta gatgaaattc atagattgcc tgctgctggg
     7981 caagaaatct tattttcatt aatagataga ggagaattta gaaggttagg agaaagtagt
     8041 tctaatagaa aggcaaatgt tctcataata agtgctacaa cagaaaatcc agattctaat
     8101 ttacttcaaa catttagaag acgtatacca atggtaataa atttacctaa tatgagtgaa
     8161 agaccaaaat ctgaaagata tgaaataata ataaagtttt ttgaaagaga agcaaagcga
     8221 gtaaacaaga attttataat aactaaagaa gttatgtgtg cacttatgaa ttataagtgt
     8281 actggaaata taggtcagtt aaagagtgat atacaagtaa cttgtgctag agcttttagt
     8341 aaatcgatat tttctagcga taaagtaatt atagatttag atagtttaag ggattatata
     8401 aaaagtggat attatgattg taattgtaaa aatgaaaagt attctgagtt ttcagttgag
     8461 gatatatata tagatattag tgaattggtg agtggaaaaa atagaatttt atctgataat
     8521 gagataagtg agatatataa ttatgcagaa aaagagctta aggttttgga aagtaagtgc
     8581 ttttcagaag aagagttaaa aaatgcattt atagaaaagt tagataagaa gtttaatgct
     8641 attaaaaaca ataaaaattt atctgataga agaagtcgaa tagactgggg aggacaatta
     8701 aaagagtcta cactggagat aatggataag gtaattgtat ttattaagga gcagtttaag
     8761 catgtaaatc aaggtctgta cttggctcta gcaattcata ttgagcatgc tataggtagg
     8821 ataaaagatg gaaaaactat aataaatcca agtttagata aaataaaagc atcaatgcca
     8881 atggaatatg agttgtcaag atatataatg ggtatagtag aagatttgac acaagtaaca
     8941 tttccagaag atgagcttgg atatttggct tattatataa ataaattttg ttataatgag
     9001 gaaagtataa aagataaagt taaagtagta atagttacgc atggtaaagt tggaattgaa
     9061 atgtctaaag ttgtaaatca tattttaggt atagaatgta cactaggtat agaaatagca
     9121 cttacagatt caccatcaga ggggatagag catgtattag aagagcttca aaagatagaa
     9181 gctagaaaag gtattctagt tctaattgat atgggttctt tggtaattct tggtgatgag
     9241 gttgaaaaaa ggcttggaat tagatgtaaa actgtaaata gagtagatac attattggca
     9301 atggaagcag gaaaacttgc aaccattgaa gggaaaagtt tagatggtat aatagctgat
     9361 ttaaagaaaa ataagaatta tgctatgata aatacaaata aatttagtta cagaaaaaac
     9421 gagtatggaa agaaaaatgt aatcattact ttatgtttga gtggtgtagg tacagcttta
     9481 aatttaaaag agcatataga aaaacaaatt aaggaatatg acactttaat tgaagtaaag
     9541 ccagttgctt ttttaaataa taatttagaa gaagagttaa atgatattga agctgaatat
     9601 aatattattg ctatctgtgg aactattgat atagattata aaaacgtgcc ttttatttct
     9661 tataatgagg tacttggtaa aaatggtatt aataaagtat tagagcattt aaatagtaaa
     9721 caaccagctt taaatattga aaaccagttg aataatttaa tacatgaaga tttgatttta
     9781 tacaattttg aaggaatttc aaaggagtat ataattgata ctcttgtttc aaaacttgaa
     9841 gaaggtggat atgtcgactc aaagtatata ttaagtgtat ataaaagaga agcaatggga
     9901 agtgttgtaa tggcctctaa ggtagccgtg ccacatggac ttccagaaaa cgttataaag
     9961 cctgcaatag caattgcgag gttgaataaa cctatagtat gggacaataa atttatggtg
    10021 gatttggtgg tactactggc acttaaagaa aataataaga aagaaatacg aagcttattt
    10081 tcaaagatta atgaccagca tactttagaa gttttattaa atactgaaga taaaaatgaa
    10141 ataaaagaac ttttaagcta attgttagta ccgagaagaa agacaaaaaa atttgcttta
    10201 gtataaaatt taaaactact caattgctaa gtagttaatt actctatata aggctagttc
    10261 tatgaaattt atttatatga ttttatagaa ttagcttttt tcttggtaaa taaaatatat
    10321 gaatttattt atgtaaaaag taaattttat tggacaattc tataaaaa
//
LOCUS       DAFKMX010000141        10290 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFKMX010000141 DAFKMX010000000
VERSION     DAFKMX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.141"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..838
                     /locus_tag="LZB08_002798"
     CDS             <1..838
                     /locus_tag="LZB08_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316287.1"
                     /translation="IISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQ
                     NLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQL
                     NAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELAKS
                     FIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESLSNSSKHGKATEIRIFITFNTSN
                     LIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDF
                     FE"
     gene            831..1478
                     /locus_tag="LZB08_002799"
     CDS             831..1478
                     /locus_tag="LZB08_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316288.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1607..2542
                     /locus_tag="LZB08_002800"
     CDS             1607..2542
                     /locus_tag="LZB08_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316289.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPKSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2556..3653
                     /locus_tag="LZB08_002801"
     CDS             2556..3653
                     /locus_tag="LZB08_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316290.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3656..4720
                     /locus_tag="LZB08_002802"
     CDS             3656..4720
                     /locus_tag="LZB08_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316291.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4964..5461
                     /locus_tag="LZB08_002803"
     CDS             4964..5461
                     /locus_tag="LZB08_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBM7316292.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(5637..5885)
                     /locus_tag="LZB08_002804"
     CDS             complement(5637..5885)
                     /locus_tag="LZB08_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316293.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5951..6967)
                     /locus_tag="LZB08_002805"
     CDS             complement(5951..6967)
                     /locus_tag="LZB08_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBM7316294.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7025..7792)
                     /locus_tag="LZB08_002806"
     CDS             complement(7025..7792)
                     /locus_tag="LZB08_002806"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBM7316295.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYESIEKYGITPIHRKSFLKNIL"
     gene            complement(7860..10049)
                     /locus_tag="LZB08_002807"
     CDS             complement(7860..10049)
                     /locus_tag="LZB08_002807"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBM7316296.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3594 a         1579 c         1184 g         3933 t
ORIGIN      
        1 tataatatct tctcttatta ttagtttaga taaaaatata tatttggcac tggagtcaag
       61 ctctatattg ctaattataa ctgtactttc tatatatata gaaaatgaga atttatcaaa
      121 attatacagt caaaatcttt atgataagct tagaatttct aaagatgaat taaaaaaagt
      181 taatgctgat ttggaaactt atgcaagttc tatagaagaa ttagcaattt taaaagaaag
      241 aaatcgaata tcaagagaaa tacatgatag tgtaggacat tctctttcta caacaataat
      301 acaattaaat gctatagaaa aactcttaaa agacaaacct ctaatttatg acttagtaca
      361 tgaacttaga gaatttgtaa ctgaaagttt tcaagatgta agaagggcta tatctgagtt
      421 aaaacctgtt gaatatgaaa attaccaaag tttatttaaa atcaaagaat tagctaaaag
      481 ctttattaag cttacaaata ttaatgttaa attaactatt tcaaaaaata cttggaactt
      541 atccagaaat caatctataa ctttatatag acttatacag gaatcccttt ccaactcatc
      601 taagcatgga aaggcaactg aaatcagaat ttttattaca ttcaatactt caaatcttat
      661 aattactatt agtgataatg gtataggatg tggcaatata aaaaaaggaa atggacttaa
      721 tagtatcaat gagagaatat atgaattaaa tggtaaagtt gaatttgata attctaataa
      781 tggtttcaca attagagctt cttttcctaa attttcaggg ggtgattttt ttgaataaaa
      841 ttaaagttct tatagtagat gacgaaaaat taattagaaa aggtcttaaa attatattat
      901 catcttataa tgatttagaa attgtaggag atgcttctaa tggatatgaa gctttagaat
      961 tttgtaaaat gaatgatgta gacatagttc ttatggatat acgaatgaaa gtatgtgatg
     1021 gggttctagg tacaaggctt ataaaggaat ataataattc tataacatta ttaattttaa
     1081 caacttttaa tgatgatgaa tatataaaag atgctatgaa gtttggtgct tcaggatacc
     1141 ttttaaaaga tagctctgat gaagtattgc atgaagggat tcgttcttca ttttttggca
     1201 atattgtgct tgataaaagt gtagctgaaa aaatcatgac ctctgaaaaa acggttaaac
     1261 aagagcatct ttatgacatg tacaatttaa cagaaaaaga aatatctata attagactaa
     1321 ttgcaaatgg acttaataat aaagaaataa gtcaagaact attcttatct gaaggtacaa
     1381 taaagaataa tataactaat attttagcta aactagaact tcgtgacaga actcaactag
     1441 caatctttgc tttcaaaaat aaaattgtaa tagaataaat ctaaaaatat aaagtgactt
     1501 aagtcacttt ttttagtgac ttatagtaat agaattataa ttcacaattc tttattataa
     1561 tattatagct ttttaactta tacaaaatta ttttggaggg attactatgg atatagtcag
     1621 agtaaataat ataacaaaaa gatttaatga taaattagta cttgataaca tcagcttttc
     1681 tgttaaaaaa ggtgagatat ttggacttat tggcccaaat ggtgcaggta aatctacact
     1741 tataaatata attacaaatc ttatgttacc aaatagtggt agtattcaaa tcaatggttt
     1801 agatttatca aaagattata taaaagcaaa gtctataata gggttagttc cacaagaatt
     1861 agcaattata gaaactctta ctccttttga taacttagag tactttggag ctttctacgg
     1921 tttaaaggga aaactactta aagaaagaat cattgaagca ttagaagtta caggtcttac
     1981 tgaagtcaaa aagaaaaagg tcaaaaaatt atctggtggt atgcaaagga gattaaatat
     2041 tggaatagca ctcttaaatc atcctaaaat attaatattg gatgaaccaa ctgtaggtgt
     2101 agaccccaaa tcaagaaatc atattttcaa ttttataaaa gatatatcta aaaaacatga
     2161 aactactgta atatatactt cccattatat ggaagaagtt gaacaccttt gttctaaaat
     2221 ttttattatg gatgaaggta aagaaattgc ttttggagat aatgattatt taaaatcact
     2281 cgtatctaca aatacaaaat taattatgga aataaaaaat ataaatgccc aacttatctt
     2341 tgatttaaaa aatactaaag gtgttatatc tgttttagaa aataattcat tattagaatt
     2401 ggatatagat aaaaaattac aacttacaga tatactctct gttattgaca aaaacaattc
     2461 aaaaataatg aaaatttctt atgaagaacc tagcctagaa gacgtatttt taaatctaac
     2521 aggaaaaaat ttaagggact aatggaggtg attttatgaa attatacatg agtgtaaaaa
     2581 ctatgctaaa gagtttaaaa agctccttta tacttaattt aatatatttt ttagctttgc
     2641 ctctgatttt atcttggttt cttgggatgg ttacagaaag catgtttcaa aaccctataa
     2701 aaacagaatc tacccccatt gttatatatg acaaagataa cacaagatta tcaaatgatt
     2761 tgacaaagta tttgaaaaat gatttatctt atatattaac agttaaaaaa gatgattcta
     2821 aggcagaatt aaagcttact atacctaaag gctatgagag tagtttatta aatgaaaaat
     2881 caaatactct aaacatagaa aaacttggaa ctcgtgatga tattgcaata ttgcttcaag
     2941 atatattaga tacttaccat gaaaaatttt atttaaacaa ttctcagaaa ataagttcag
     3001 aagatttttc taaattattt aataaaaatt ctattgatac ttctataata aataacaatg
     3061 ttaaacagaa ctcttatgaa tactttgctt tagtttcact aggatttctt gtaataattt
     3121 ttataatgaa caatattttg tcaaattata taagtgaatc aaagggactt tcaaaaagac
     3181 tttattctat gcctataact agagtacaat tcttaattta tgactttgta ggtttatgga
     3241 tttattcatt tatattctta ttattatatg ttttattctt tagaatactt ggaataacat
     3301 ttaaaggtaa ttttgccata ttgctcctac tttgtgcttt atcatcttat tttatgactt
     3361 caatatctac ctttgtaacc agctttttta gtaaaaaata cggtacagca atagtttatg
     3421 cattactctt tttacagaca atttttggag gtatcttcag tatgataagt gatacattta
     3481 ctaagcttac aagtttatct cctacctatt taataggaga attgtttagt aattatgaaa
     3541 catttaaaac tatagattca ataggagatt taattttcac atgtttaatt atctctacaa
     3601 tattaattgc acttgctatt gtaaaggaaa aatataaatg gagggaggtt taataatgat
     3661 tagattaact attgcaaata taaaaagata tctaaaaaat cataatcttt taattaatat
     3721 ggttatgctt cctattatac taattttttc acttaatttt tttatcaaca actcaggaaa
     3781 tcaatcaatg tacttttctc cagtagcaat tgtatctgac tcttcaggaa agtatgaaca
     3841 taagttaata aatagttcta aattaaaaga aaattcattt agattaaatg aacaagataa
     3901 ggctatgaat ctattaaaaa ataataaggt ttctgctgta ttcgttttag ataaaaattt
     3961 ttcaaatagt atagataaat tacaacgacc tgtagttaaa tgctttaaaa ttgaaaatgg
     4021 tggtggtagt ctttgggcag aatcacaaat agaatctttt attactaaat cgttaaaatt
     4081 gaaagtggat aaaaatatag atgataaatt gacaaagaca aatataattg ataacaaacc
     4141 agataagaat aaaggttcat ttttagtagt cttcttgata tgctatttta tgtatattaa
     4201 tgcggcacat cttgcttctg atttatttac attaaaaaaa tcaaatgtgc taaaaagact
     4261 tatctctaca gataataaag atataaaaat aattttcagt atatttttag gactattttt
     4321 tattcaatca attgtataca cttttgctct attatgtttt tcaattatag aagactttaa
     4381 tttgagtctg aatgtattga tgattgtact agcaaatagt tttgtttcca ctggatttgt
     4441 attttgggta gctagagtat ttaaaaatga aagttctata agtcttatat ctacatttta
     4501 ttctttaata ggtctagcta tatctatctc tagtctaata ccatcaatgg ataaattatc
     4561 ctttatgact aatctttcaa agttcacacc attttattgg actatagatg ctattaaaaa
     4621 caatggtagt attttaataa atataatagc tctaatatta attggaatta tatttgtaac
     4681 tgcaggaagt cttaaactaa gagattttgc taaaaactaa taattattaa atataattag
     4741 tatctatttt tatataattg gaaaagaata tttcaacata ttaaattatt ttatcttata
     4801 tttttctctt tagtttcaca caccaccaag tctaaacttt atattatata ttaatatttt
     4861 tgacttgtca agttatttat gttataatat taatcattag ataattaaag cattgtacct
     4921 aagttgcaat atctaaaatt caatttggag gtataactag catatgtata aaattggtga
     4981 aagtgtaatg tatccaaaag agggagcctg ctctgtaaat gatattgtaa caaagaaaat
     5041 aaatcatgag atgcaaaaat actatgaatt aagtgtcatt tttaatagta accttaaaat
     5101 ttcgattcca gttctaaatg cagatagaat tggcatacgt ccagttatgg atggaaatga
     5161 tgttgataat tttatccaat ctattaataa aacagatggt gtatggattt ttgatagaaa
     5221 agaaagactt aaactgtacc aagataaatt tcattcagga gatgtttttg aaattgtaaa
     5281 attaatcaaa atgcttatga tacaagacag ttcaaaacaa ttgtgtagca cagataagga
     5341 gtttttaaat aaagctcaaa aatttgcttt atcagaatta gctgctgctc aatgtaaatc
     5401 ctatacaatg gtattagagg aaatgaaaaa acatatatta aattcaaaga atataaatta
     5461 aaccagtttt ttaaactggt tttatttttt atactaatca tccatgctac tttagcttat
     5521 tttaaactgc ctaatctaat attaataaag ttatctcaat tagatagatg tattttattc
     5581 aaataaaaaa aactaagagt aaatcatatt actgatttac tcttagctaa taaaatttaa
     5641 tcttcttcaa gttctttttt ctcttgtaat aatacactta attcatttgc ttctaaaact
     5701 tgtgcctctg caatatcaag taactcttgt tgccattcca cacatttatc tttgtaatca
     5761 aaaggtagcg ttctgtcaac tatgtactta tcaatacagt cctccgcact tttaattatt
     5821 tcttttcttt catcaataaa tttttctaat tctactaagg cttcttcata agcttttcta
     5881 ctcatataaa ttcccccttt gtacaattat tctacattaa aatgattttt cctctttttt
     5941 gtataaaatt ttatttgtat ataacttttc catcaatatc ttttatagtt tgacatccag
     6001 ttaatatcat agaactactt agctctgatt gaagtttatt tacataagtt tctacaccat
     6061 cagttgcacc accaaaagaa gcagtcacaa aaggtcttcc tattaatact gcatctgcac
     6121 ctaaacctat catttttagt acgtctaaac cagttctcac tccaccatct gcaagtattg
     6181 taactttacc tttaacagca tcagcaattt ttggtaaaac ttcacatgct cctggtgtac
     6241 agtcaagcac tcttcctcca tgatttgata caactattgc atccacacct gcttctactg
     6301 ccattaaagc atcttcaaca gtcattatac ctttcaatat aaatggtagt ttagtagact
     6361 ttactaactc ctttatttgt ttaacatttt taggaagtac tggttttcca tgtaatgata
     6421 aagttactag accacaggca tctatatcaa ctccaactgc aaatgcacca gcttcttcag
     6481 ctaatcttat cttttttata atattttcat tatcccaagg tttaataaat acaattccag
     6541 ctccattata ctttttgact acatctaaat tttccattaa aaaagcatct actgccgtat
     6601 caccaaccat tgcataaata ccactattag cacatcctgc aactactggt tctatgtaat
     6661 ctctttcatt aatttttcca cccatattta aagtagttcc tgttactgga gctgcaaata
     6721 taggtgcact cattttcttt ccaaacatct ctatagatgt atcaggattt gatacatcat
     6781 gtattactct catatttact tttacttttt ctaaactctt gctattttct ataaaagaag
     6841 aaccacttcc tttacctccc atgccaggca cttcacctgc acaagcaacc ccattacaaa
     6901 ccttacaaac tttacaactt ccatttaaat tttctctagc actttttaat aattcattat
     6961 attccatctt taatccccca ttaactatag taatttatat tatatacatt atatcatatt
     7021 atatttacag tatatttttc aaaaaacttt ttctgtggat aggagttatg ccatattttt
     7081 caatagattc atagtgttct ttagttccat atcctttgtg agatttaaac ccatattctg
     7141 ggtatactct atcatattgg tacattatac tatccctagt aactttagca agtatacttg
     7201 ctgctgctat tgatatagat tttgaatctc ctttcacaat tggattttga gatatgtcaa
     7261 tacctggtat tgttgctgca tcaactaata aataatccgg tgcttttttt aaacaattta
     7321 tagccttttt cattgccata tatgtagcat ttaatatatt aaattcatct atttcttcat
     7381 tgtttactat acctattcca taatctagtg cttcttcttt tattacttca aaaagttcat
     7441 ctctttttgc ctcactcagt ttcttagagt catttacacc ttctattttg gtatcttttt
     7501 taaatactac aacagaagct acaacaggtc cagcaagtgg tcctcttccc gcctcatcaa
     7561 ttcctccaat atataaataa cctttatcat agccttcgtt ctcaaatata tttatagttt
     7621 ccaatctttc ttcttctttt ctaatattat ccagcttttt tgctagtttt atagctagac
     7681 cttgtacaga ttttctttca tcaactctta acagctctat atattccata tatttttcta
     7741 cttctaaagt ctctattatt tctttaatct ccctcacact tttatcctgc ataccattcc
     7801 ctcatctttc gtttttaaaa atttaatttt taaatatatt aaaatttttt aattagagtt
     7861 taattgcatc cactatagcc acattttaaa catatactac atccacctgt atgtgcaatt
     7921 ccttcacttc cacactcagg acacacaaat ttttcatctt cttcaaaatt tttatttatt
     7981 atttctttgg ctttttcatc tttattatct ttttcttttt tattattttt ataatattct
     8041 tttttactat tttctttttt atcttcttta ttattttctt cctttttttc atttattaaa
     8101 attccactcc ttttacatcc atctctaaat attgttactc ctttaagacc agcctcccat
     8161 gccttaatat atatattgaa aacatcttca actgtagttt caaaaggtat atttattgta
     8221 gatgatatag aagcatcaat atacttttgc catacttttt gcatgtttat tctttcttca
     8281 tatcctatat tcatagcagt aacaaaaaaa tctggtaaac attcttcatc tgtaatacct
     8341 ttcttgtcca tatactcttt aacaattgga gtatatactt tataatacac atcctcatca
     8401 tgcaagcttt cagtttttct tatatatgaa agattaaata taggttctat tccaccacta
     8461 atacctatca ttgtagatat agaacctgta ggaggtatgg ttaaaagttg tgaatttcta
     8521 agcccatact ttttaaccat ttccttagtg tcttcattta gattttctat aaaaaactta
     8581 gatttagaaa tattttcata tttatactca ctaaatgttc catattcttt agcaattagt
     8641 gctgattgct tcacagcttc atttaacata gtcttaccaa ttaaatcaca tagttctaaa
     8701 gattcatcag aaccatatct tattcccatt tttataagca tatcagctat acccattaca
     8761 cctaaaccta tctgtctata cttatgtaca ctatctcttt gttcttgtaa tggatgcaag
     8821 ttcaatccct catctaagac atcatttaaa gctactacag cttctctaac gcaagatttt
     8881 aatttattaa tatcaaaagt agcatgttca ccaaatggtt gtattataaa ctctgacaag
     8941 tttattgagc caagtagaca acttcctcca cttggaagtg gttcttctgc acaaggattt
     9001 actcctgaaa agcaaaagtt cttatcttca ctcataaaat tatatgtttt tattttatcc
     9061 caaaacaata tacctggttc tgcataatcc cagttatttt tcacaaattt attaaataaa
     9121 tcataagcat ttactatctt aactattttt tctccagtag tttcaacttc aaaagtacaa
     9181 ttatataact ttttttctct tacagcttgc ataaattcat ctgttactct tacagaaata
     9241 tttgctttag taaccttctc caaatctgtt tttatatcta taaagtcttc tatctctgga
     9301 tgatttacat ccatagaaat cataagagct cctcttctcc ctctttgacc aattaagcca
     9361 gttgtcatac tgtaaagttc cataaaagag actgctccag tcgtatgttt tgcagcattg
     9421 ttaactttcg ctccttttgg tcttagcttt gaaatatcaa ttccaactcc tccaccataa
     9481 ctaaaagttc tagcaagttt ttttgcagta tcaaatatat cttctatgct atcttttggt
     9541 ggttctatta cataacaatt agaatatgtt actttttttc cctctttctg taatcctcta
     9601 tttgctaaaa ttcttccagc aaatagaaat cttttttgtc ttattagttt tgatattctg
     9661 gaactattat ttgaaattct ctttatccat tcatcaaaac tctcaccatt atgtctatac
     9721 ttgttcttcc atattgattt ttgtaattca tcaaactccc aagctttagt ccttatttca
     9781 gagcgtcttt ctctatataa tatgtagtgt tttgctatat cttttctatt tgattccata
     9841 agtagtactt caactatatc ttgtatttct tctacggata agcatttttc catagaatta
     9901 tagtcattag ctatttttct tgatatatca tatgccaact ctctatctac tccaccttca
     9961 ctatttatac tagctttata gacagcatct tcaatcttac ttttactaaa aagttctttt
    10021 gtaccatctc tttttattat gtatttcatt tttaaacctc caataaatta gtattaaatt
    10081 aatacacaca ttaactataa ctctaaacaa actttttata tatttgtata ttttcatatt
    10141 aattatctaa cataatatat attatactat tttatattca atataacact ccttatagtt
    10201 ttcttaactt gtaaaatatc ttcctaaaaa actcaaaaat aatataaaaa agtatataaa
    10261 agtcaaatta tactcttata tactttattt
//
LOCUS       DAFKMX010000142        10175 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFKMX010000142 DAFKMX010000000
VERSION     DAFKMX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.142"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            225..1106
                     /locus_tag="LZB08_002808"
     CDS             225..1106
                     /locus_tag="LZB08_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7316297.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1154..1464
                     /locus_tag="LZB08_002809"
                     /pseudo
     CDS             1154..1464
                     /locus_tag="LZB08_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1724..4291)
                     /locus_tag="LZB08_002810"
     CDS             complement(1724..4291)
                     /locus_tag="LZB08_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBM7316298.1"
                     /translation="MIYYVLPLKHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQSFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFYE"
     gene            complement(4294..4899)
                     /locus_tag="LZB08_002811"
     CDS             complement(4294..4899)
                     /locus_tag="LZB08_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316299.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSLEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5273..6733)
                     /locus_tag="LZB08_002812"
     CDS             complement(5273..6733)
                     /locus_tag="LZB08_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBM7316300.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7066..7644
                     /locus_tag="LZB08_002813"
     CDS             7066..7644
                     /locus_tag="LZB08_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316301.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNEIQNKLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYLPNNHDYISLINKLPQDMFSQLEYNNIQSFYDLIDKYNLKFKIEKEKAFGICVA
                     LLSGLLVKENIEYNHIEVFDFMVDNLVEHILE"
     gene            7935..9185
                     /locus_tag="LZB08_002814"
     CDS             7935..9185
                     /locus_tag="LZB08_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7316302.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLTSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKVTNLHIPFIICALIFVPMAVSVYPFILNIGFPVPFLIFIVSSMLIFF
                     TTSLTSIYVITITQLKTPNELLGKVMSILFSGTSIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9346..9915
                     /locus_tag="LZB08_002815"
     CDS             9346..9915
                     /locus_tag="LZB08_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBM7316303.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3406 a         1735 c         1489 g         3545 t
ORIGIN      
        1 atattgtata ctttcttttt aattatttaa tttttattat ttgtacttat ttaaggttac
       61 atctagttat aaattggaca atttttatgt taattttatt tatgcaaatt aaagtaactt
      121 acaaactttc cacaataaaa aacctttttt attcgttaaa ctgctccatc attggtttag
      181 caataaatat attttttagg tctttaatgt caatagtgtt aaatatgcca gcatatttat
      241 ttgatagtaa ttctattaca aatatttaca gagtattttc tatattgctt agttttatag
      301 ttacaggatt atatttcagt tataaaaatc ataaaaataa taagattaac tttgaggcag
      361 ttttagagga caaaaaaaac ttaattttct tattgagcct tatgtttgga atgtacttgt
      421 atctatgttt gagtttgtca atatactata ctgttggaaa tgtattgatt ttaaaattat
      481 atggaatcaa gtctgccata atagttttgg taggtaacta tattgcagaa agatatagct
      541 ttagaattac tcaaataaaa ggttatgata ttaaaaatca aatagaatat gtagaaagtg
      601 taaagacaga gtatgaaatt aataaattga atgatattgt atatacagat ttactgacag
      661 gcttctacaa tagacctttt gtgaatcaaa aactatcaga atggacatca aatcactaca
      721 aatttacctt atgttttact gatttaaaca gcttaaaacc tgtaaatgat aaatttggac
      781 accaatttgg tgataaatac atatccacta ctgctcaaat aataaaaaaa cttataaatg
      841 tagaaaattc cttactattt agatatggtg gagatgaatt tttattcctt ttacagaata
      901 caagttgtgt agaagcagaa aaaataatga ttaatataaa ttatgaatta tcaaaattaa
      961 gtaatactga cgagtatcct tatgctcttt ccataagcta tggattagtt gagtgggatg
     1021 gaatatccga tattgaaact ttaatatcaa aggcagattc tttgatgtat gaaaataagc
     1081 ttaaaaataa atctacttta aactagctag ttaagatatt ctacaaaata tatgttataa
     1141 gagagtacta agaatgtcaa aatatataga ttttgcaaat attgaataca gtttactaat
     1201 gaatgcactg gatgtaagtg ttagtaaaca cctattagat gaacacttta ctgttgtttg
     1261 ggctaatgac cgttattatg aaatgttcgg ctatacctag ctaaaaagtt aaaaatgacc
     1321 actgtagccg agggaataga gaccagtttt caaaaagatt tcttacaaga aataaactgt
     1381 gatatgttac aaggatacgt gttctctaga cctttaccaa taaaagactt tgaaaaactt
     1441 atgtttcaaa actcctccaa ttaacaatat aaaattgctt attataagtg atattattgt
     1501 atcaacaaat aaaggaattg tctcaaaatt gattttttct aatcaaattt ttattttgag
     1561 acattctcat ttttttgcat aagaaaatac ccaccaagct acggtttttc ttaccgctgt
     1621 tcggtgggta ttagtagtat tatgaaatct gaaaacttta agtattacta cttccgctag
     1681 cgcaatcaca aaggtcgaaa gcttcaaagc gaaaataggc tttttactca taaaacgtca
     1741 ccgtacctgc caacccgtcc accgttatca tttgtccgct cttaatagtt cgtgtggcaa
     1801 tgtgtgtagc cataacggct gggatgccat attcacgtgc gacaatgctt gaatggctaa
     1861 gaggaccacc aatgtccgtt acaatcgcac ttgcagatga aaataatggt gtccatgctg
     1921 gagttgtggt aactgcaacc aaaacacttc caggttgaaa gctttcaaaa tctgctggac
     1981 tattcagtac acaagctgga gccgtcacaa caccagtact ggtaccaatg cccgtcaaca
     2041 caattttccc atctttctgc ttctgtggtg cttgtgatat agtttttttg tttttttctg
     2101 gcaacttgga tggtgacaca taacccatgt attttttaag ttcagctttt cgaacaggta
     2161 ttatcccgtt catatcagat agagacttgt ttttatccaa ctgctctata agtacctcca
     2221 attccgattt tgtgagccaa taaatatcat ctaaatgtga aatagctcct cctcgtatga
     2281 atcgttctga gatttcctga aacatacaac gaatcagagg atgtcccatt cccatcaaat
     2341 aaatagcatt ttcacgcata ggagaggttt cctgtgccca atggagcaac ttaaaaaata
     2401 gttttttacg caatccacct atgtgttgta aaatctcttc ttctgcatgt tttctacgct
     2461 tttcaaacgt ggtctggcgt aaaaaaggac tttccccttt tccttccaca aaggttttta
     2521 cggattcaaa agttggtgta agtgtttcct gcggtgtgga atacgcaaaa tcaaactcat
     2581 aagcggtacg accaaactct ttaaagtact tattgattcg atttttccat tctatccata
     2641 cttcctgtga aactccaaca ggcacaactg aagacatgaa atcttctgct atctttattg
     2701 caggactgtt ttgcagataa aggttgatac tgttattttg ctttacccat tctgaaagac
     2761 cccacaactc cttttccgat tgcagaacaa tcgtatcaaa cccaagcagg aaaacggaag
     2821 tatccgttat accagcacgt cgaactgctc cttgaaataa ctttgtaaat aatgtttcac
     2881 tgatagaagc agcaggcaat gtaagctgaa tccttgtgaa ataaatacat gcggcataaa
     2941 atattgtctg aataccctcc ataatttgat gggcgttcat agtatgcagg ggcttttctt
     3001 cccattgttt aatagcatcc tcatatcctt ttcgtgccgt ttcccagcgt gcaacgctgt
     3061 tcataagtgt cctacgtaaa gagcgtggag aaagggattt gatagcaatc aaaagtggct
     3121 ttgatttagc agaaagataa acatatccat taataaccgt atatgccccg ttttttggta
     3181 ataatttttt cgcactttta ccaaacatat ctatccataa aagctctgat gcacggttta
     3241 cgatttcaag accaagcgtg gcgaacaaag gtgtaacagg atttggcaag tgttctgcca
     3301 aacttccctt tgtgtataca acatcctgtt ctggcaatac ccattctggt ggcagaacag
     3361 taattggacg agcctgaaca atgtacaatt tatctttttc cagtgcccac tctacatcca
     3421 taggcatttg ataatacttc tcaattttct ttccaagctg tatcagttgc attacctgat
     3481 tacgagtaag agcatatttt tttctcaacc tttcaggaac catggtttct tctgttcctt
     3541 ctgaagtacg aaccgtcata atctctttat ttgccacttc atacgatata attcgttcgg
     3601 agtccttatc cactacgatt gtatcgggtg taactaagct agaaaccacc gcttcaccaa
     3661 gcccccatgc agcgttaata atcatttcgc tacgtctacc attgattggg tttaacgtaa
     3721 acataatacc agatgaatcg gaaaatgcaa gcttttgtac tacaacagca agtgcgacaa
     3781 tctcgtgctt tatattattc ttcacacgat aagcaatagc acgagctgtc cataaggaag
     3841 cccagcatct ctttacagcg tcaataactt tatcttcacc ttgtatgtta agataagttt
     3901 cctgctgacc tgcaaaagaa gcgtcaggta aatcttcagc agtcgcagac gaacggacag
     3961 ccaccgatat gttccctaat ccagcataag ccattttaat tgcgtccgac acttcctgtg
     4021 gcatttctcc attatggaaa agctccccta ttttcttaga tacatcttca agttggctgg
     4081 tattattgga atcaatacca tctagcagtt tattaattcg agactgaata tggttcgtct
     4141 caacaaaact ctggtatgaa gcagtcgtaa catgaaaacc gtctggcaca gggatgcctg
     4201 ctgtcaatag ttttgataaa gacatccctt ttccaccaac aatttcaatg gttgcttgtt
     4261 tatgtttcag tggcaagaca taataaatca taatcattca cctccatcat tcataattcc
     4321 gtgcagaaat atattcattg tttcatccaa cattttttcc aaactatccg atggtcgtga
     4381 cgtgtagctg attgataaca tggagttaat taacaaacgt gtcacaagag cggtatccgt
     4441 tttgcgaaag atgcctgtgg tcacaccatt atcaattacc gattttataa tatcttgata
     4501 tgcataacga gcatctttaa gtctctttcg atactcttct tctaaataat cagattctgt
     4561 atttaaccac atcagtacag ttctatactg ctctggaaat ctaagatgat taagcatgat
     4621 ttttctaagg cactgttgtg gtgaaggttc accatcaata atcctatgaa cttgttggat
     4681 agttttttct atttctttct caactgcata tacaacaatc tcgtctttcg ttttgaaaaa
     4741 atcgtataga cttgactttc ccatgttggc taaaactgca atttctctca ttgatgtttt
     4801 ttggaatcca ttttcagcca tgagactcaa agcaacagca actatttttt ctcggcgttc
     4861 tgcttgttgt tcgggtgtaa gcacaattcc ttttggcata gggcgatacc tccttttagc
     4921 gaccactggt cgtttctttt ttatttatca tacagcgacc aacggtcact tgtcaaggtt
     4981 aatttttttc attgtatatg gtactcgaga taaaaatgtt cattttagaa attgctctat
     5041 ttaagtagaa ataggcttca tcattagaaa acaagactat aaatttgaga tatttatcat
     5101 gttcaaattt catcaatatc aaagtttaag atattaataa aaaaagaatt actaagaaat
     5161 aacatataaa gattcaaaaa aggagttgaa cttaaaattc aattacaaaa aatcaaattt
     5221 aagacaactc ctttattctt tatctctact attctttatc tctatttatt tattacaact
     5281 ccctaacatt taccactact gccttaggtc tagtcatagc ctcgattgaa tacttaacac
     5341 cttgagttcc cataccagat gcctttacac ctaaaaatgg gaagtggtct ggacctcttt
     5401 cagtcttatt atttatttga acagtaccaa cttcaagttt atcagctata taaaatgcct
     5461 tatcaatatc acttgtaaat actgatgatt gaagcccata ctctgattga ttagcaattt
     5521 caattgcttg atttatatct ttaactctta taattggaag tactggacca aatggttctt
     5581 cccaagcaat tttcatatca acatttacat tatctaacaa tgttggatag aataaattat
     5641 tttttctaac attgcctgtt attaactttg ctcctttatg taatgcatca tcaaccaacc
     5701 cttgaacaaa atcagttgct ttattatcta taagaggagt gataactact tcatccattg
     5761 gatttccaat tgtcaacttc tctaccaatg gctttatttt tccgactaat ttatccgcca
     5821 cactctcttg tactaaaatt ctctttactg ccgtacatct ttgtcctgaa tatgaatacg
     5881 ctccagatac tatattctta gctgcaaaat ctaagtctgc atcttctaat actatagccg
     5941 catcttttcc accaagctcc aataataatg gtaccattcc agatatttta gatatatgct
     6001 gaccaacttc agtactacca gtaaagttaa taaaattaat tcctttatgc gttacaacat
     6061 aatcccctat ctcactaccc ctacctgtaa tagtattaag tactccatct ggtatcccag
     6121 cttttctcat aatttctacc acatgaagtg cgctaatagc cccttgtgta gcaggtttta
     6181 atacgactgc atttccacca ataagtgctg gagcaatctt tgacattgat aaatttacag
     6241 gataattaaa aggagatata gctaaaacag tacctaatgg aacccttgaa acataagaca
     6301 tcttatttct tgttccacca gggaaattat ctccactaat ggcttcaccc tccatatttt
     6361 ttcccacatc agctgtgtat cttaaaaaat ctgctgttct ctcaacttct gcccttgcag
     6421 attttatgtc tttggctatt tcaagtacca gtatattagc tatctcatct atattttcta
     6481 acaataaatc agcagcctta tgaaatatat tggctctctt gtatacaggc atttcagccc
     6541 atacttttat actctctttt gtgtttttta taacctcatc tacctcatgt tttgtcatag
     6601 attgtacttt cccaacaaga gaattatcta ttggtgagta aatttctatt ggctttctcg
     6661 aattagattc tacccatttt ccatttatta aatttttata tacattttct tttgttttaa
     6721 gttcattaaa catattttta ttcctctctc tgatataact ttgtgtattt aaattatagc
     6781 catttattcg ttatgtaaac atttcttttt ttcataaacg tttacctata aaaaaaattt
     6841 aaagttaacg tttttctaca ttcttctatt ttaataacat actcgctcca aatctatata
     6901 ggtcacactt taatactaat ataccaatat actaatatat caatatacca atattataaa
     6961 cataaatcac ttgacaaatt tataagtaga caatattata tatctatgac ttaaaatatt
     7021 tttaaagtca tagatatcat atatagacag aaaggattga taaaaatggc taaaagtttt
     7081 acagaaatag aaaaagacaa tataagaaaa agactaattt cagaatgtga aatcaactgg
     7141 tctaaatatg gatacaaaaa aactaatatt gatgaacttt gttcaaaatc aggaatcgca
     7201 aagggttcat tctacacatt ctttgattca aaagaaaagt tattttttga agtactaaat
     7261 gaaatacaaa acaaacttat atcatcagta gatgaaattt taagtaatat gcctccaaaa
     7321 gaaggctttg caaaatcttt aaaaatgtta tatcgattat atgataaaaa tccattttta
     7381 tatttaccaa ataatcatga ttacatcagc ctaataaata aactacctca agatatgttt
     7441 tcacaacttg aatacaataa tatacaaagt ttttatgact taattgataa atacaatctc
     7501 aagtttaaga tagaaaaaga aaaagccttt ggtatttgtg ttgctctgct atcaggtcta
     7561 ttggtaaaag aaaatattga atataaccat attgaagtgt ttgattttat ggtggataat
     7621 cttgtagaac acatacttga ataacaattt aaaataattt ttctaagtat ttttttatac
     7681 taaataggat taatatgatt gaattaatgc aaaaaataaa caatataatt tataaaataa
     7741 atgataaact aaatatcaca caaaacttat agaccctaga gcaaacccat tccactctac
     7801 tctaagccat ctataatagt ttgtatattt ctaataatat tagatattaa agtaagaaaa
     7861 aaattcctta taataattgc tattatatca attattataa atattttaat aatcttagat
     7921 aggtaggtac atatatggta aaatcctcga acacttttaa taaaaacttc aacttgatga
     7981 tattaggtca aataatctca ttgtttggtg catcaatact caagtttgct ttgtctttat
     8041 acatacttga tataactggt aaagcagaaa tatttgctac tatactagca gtatcttcaa
     8101 tcccaattat agtattctcc cctattggtg gagctattgc ggatatattc aacagaagaa
     8161 acttaatggt tatttttgat ttttcaagta gtcttacagt actaatctta gcagtattct
     8221 tattcaacaa taatggttca atattacttg taggaattat tatgaccata ttatcagtca
     8281 taagcacaat gtatcaacca actgttcagt caagtacacc ccttttagtt gataatgagc
     8341 atttaatgaa tgcaaatggt attgtagctg gagttgcatc ccttacaaat attgcaggac
     8401 ctgtattggg aggtgtcttg tatggtgtga ttggaattaa tgcaattata gttataagtt
     8461 gtgtaagttt ctttctatct gcaattatgg agatatttat acagattcct tttatcaaac
     8521 aagagcaaaa cagcaacatt ataaaaacta tatttactga tataaaggat ggtattgttc
     8581 atatatgtaa agaaaataga tttatactaa aaatactttt tttagctata gcaataaact
     8641 tatttttaac ttctatgtta attgtaggta tcccctatat agtaaaaata actatgggag
     8701 ctagtaactt cttatatggt ctagcagaag gagcaatttc ttttagttca ctagtagctg
     8761 ctgtgagtat tggtattttc tcaaaatatc taaaagtcac taatttgcat attcccttta
     8821 tcatatgtgc acttatattc gtaccaatgg cagtttcagt atatccattt atattaaata
     8881 taggatttcc agttccattt ttaatcttta tagtatcatc tatgctaata ttttttacaa
     8941 cgagccttac ctcaatatat gttataacaa tcacacaatt aaaaactcca aatgaacttt
     9001 taggaaaagt aatgtcaatt cttttttcag gaacatcaat tgcaatgccc cttggtcagg
     9061 ttatgtatgg taaacttttt gatttattat cagatagagt gtacatcatt atactaggag
     9121 ttggatttat tacactgctt atagcatttg tatcaaagat aacattgagg aacgaaaatc
     9181 tttagataca aagaatttag atataaagat ttaattttca ctaatttctg aatttaaatg
     9241 caaaattgtg tatcagtatg ttgacgtttt agaaattgat tgatataatc aatggaaaat
     9301 aaactttata aaaattcaaa atattcagct agaggagtga taaaaatgag ttacatgcca
     9361 actttagaac aagcatggga aatattaaga aaatacaaca aggaagaatt tcatataaga
     9421 catgctcaaa ttgtatctgg agttatgaga tactatgcaa aggagtacga ccctgaaaga
     9481 gtagaatttt gggaaatagt tggactttta catgatttag attttgaaat gtatcctgat
     9541 gaacattgtg ttaaacaaag agaattaatg acagagcttg atttagataa atcaataatc
     9601 gattctacaa taagtcatgg atatggatta actggttcag atgttaaacc agagcaattt
     9661 atggagaaag tattatttgc tgtggatgaa cttactggtt taattggtgc tgctgcaata
     9721 atgcgtcctt caaagagcgt ttctgattta gaattaaaat ctgttaagaa gaaatttaaa
     9781 gataaatcat ttgctgctgg ctgttctcga gacgttataa gaagtggtgc agaaatgctt
     9841 ggatgggaac ttgataagct tatccaaaca actatagaag ctatgagaag cttgattcca
     9901 gagatggcaa tatagctcct ccatacatat accatagaag ttacatcttt aaagagcatt
     9961 tctctttttg atgcaacttc tttttatatt ttgtagaata aaaagtttaa tattttaaac
    10021 tcactatata atctataata aatactataa ataatataat gtataatagt tataaacttt
    10081 ctaaccttaa aagtttctct aatactcaat aaacaattct aatatataaa tggcattata
    10141 tgatttaatt atatatcttc aatatatata ttaaa
//
LOCUS       DAFKMX010000143        10148 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFKMX010000143 DAFKMX010000000
VERSION     DAFKMX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.143"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            133..2004
                     /locus_tag="LZB08_002816"
     CDS             133..2004
                     /locus_tag="LZB08_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBM7316304.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKSKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSRTIYVGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIDK
                     LGIAGAKNIDDVQLAEITIKNSDLNIVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTSEKDLKDAKAALGGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2111..2818
                     /locus_tag="LZB08_002817"
     CDS             2111..2818
                     /locus_tag="LZB08_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBM7316305.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            2851..4995
                     /locus_tag="LZB08_002818"
     CDS             2851..4995
                     /locus_tag="LZB08_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBM7316306.1"
                     /translation="MKISKKIVSLLTITFLTIMLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWTKSESAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDDRTKVELKRLGV
                     KNVYLIGGTNTLGINVEKQLKAENISFERISGNNRYQTSLKLAEKLSKEKEISNIVVV
                     NGEKGLADAVSVGSVAAQENMPIILSDTINGIKDTEKFIKEKNIKKSYIVGGIYSVSN
                     SVEQSLPNSKRIAGNNRNETNAKVIEEFYKNTDLKNVYITKDGMKKQSDLIDSLAVGV
                     LAAKNNSPVVLVGNKLDFTQNDVLNTKIIKNVVQVGGLGNENAVESIIDSQEETTYTV
                     KNTDELNVAIKKSDANDIIKFKPEKEKNITESFKVETDKSISVEFEGTYTQTISINMP
                     NGSITNLGDVNGTIKIMDVRSSTLVNKGEINYLDIYDNNGCRVENERDGDIWLVTISN
                     ESRDVHIENDGDINKISNSCSSVTIKNSGNIDTLSGSKEIAISGKKPKVDDTDKDDER
                     ASGINIKVEPCSIPKRDYVKVRIVTEPKNSNYKIYYKVVSKKPTSRYVGDKIKVSEWD
                     DAPSAGTSFEVEARNGFYIEAVEINTSTNKISKWGRSDSTDDGFKSIEVAKGLSANVF
                     IDGDKVELTTTRANSGCEIYYRIMSSKPTAMNVGSSITLSSWEKISSSSLELESSEVS
                     EKYIELVELEKSTNLVTRWGSTEKINIPNSSR"
     gene            complement(5031..5429)
                     /locus_tag="LZB08_002819"
     CDS             complement(5031..5429)
                     /locus_tag="LZB08_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBM7316307.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIKFLFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKVFTNIIVIVLNYLFSKMIIFKEK"
     gene            5656..8067
                     /locus_tag="LZB08_002820"
     CDS             5656..8067
                     /locus_tag="LZB08_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBM7316308.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            8364..9941
                     /locus_tag="LZB08_002821"
     CDS             8364..9941
                     /locus_tag="LZB08_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBM7316309.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTTSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         4230 a         1151 c         1821 g         2946 t
ORIGIN      
        1 taagaaaaag tttgtatctt ttggttaatt attacaataa gtctcattta ttgaaataat
       61 atcaaatata tattataatt ggtaaataag gaaaaaataa taaaaatttg aattttttag
      121 ggggaaaata ccatgaataa aaaaaatctt tctgtaatta tggctgctgc aatgataagt
      181 acatcagtag ctccagtttt tgctgcagaa actacacagg taaaaaaaga aacaataact
      241 aagaaagaag ctacagaatt agtttcgaag gttagagatt taatgtctca aaagtatact
      301 ggtggttctc aagttggaca accaatatat gaaataaaag ttggtgagac tttatcaaaa
      361 ttaaaaataa taactaatat agatgaatta gagaaattag taaatgcttt gggagaaaat
      421 aaagaactta ttgtaactat aacagataaa gggcatataa caaatagtgc aaatgaagta
      481 gttgcagaag caactgaaaa atatgaaaat tcagcagacc tttccgctga ggctaattct
      541 ataacagaaa aatctaaaac tgaaactaat ggaatttata aagttgcaga tgtaaaagct
      601 tcatatgata gtgctaaaga taaattagtt ataactttaa gagataaaac agaaacagta
      661 acttctagaa ctatatatgt aggtattggt gatgaaaaaa ttgatttaac agcaaatcca
      721 gttgattcaa ctggaacaaa cttagatcct tctacagaag gatttagagt aaataaaatc
      781 gataaactag gtatagcagg agctaaaaat attgatgatg tccaattagc tgaaataact
      841 ataaaaaata gtgacttaaa tatagtttca ccacaagatt tatatgatgg atatagatta
      901 actgttaaag gtaatatggt agcaaatggt acatcaaagt caattagtga tatttcatca
      961 aaagattcag aaacaggaaa atataaattt actattaagt atactgatgc atctggaaaa
     1021 gcaatagagc ttactgtaga gagtacgagt gaaaaagatt taaaagatgc caaagctgca
     1081 ttaggaggta attcaaaggt taaattgata gctggagatg atagatatgc aactgcagtg
     1141 gctatagcaa aacaaacaaa atatactgac aatatagtta tagttaattc aaataaacta
     1201 gttgatggat tagcagctac accacttgct caatctaaaa aagcacctat attattagca
     1261 tctgataatg aaataccaaa agtaacttta gattatataa aagatataat taagaaaagc
     1321 ccatcagcta aaatatatat agtaggtgga gaatcagcag tatcaaatac agctaaaaag
     1381 caattagaat cagtaactaa gaatgttgaa agactagctg gagatgatag acatatgact
     1441 tctgtagcag tagcaaaagc tatggggtct tttaaagatg catttgtagt aggcgcaaaa
     1501 ggggaggctg atgctatgag tatagctgct aaagctgctg aacttaaggc tcctataata
     1561 gtaaatggtt ggaatgatct atcagcagat gctatcaaat tgatggatgg aaaagagatt
     1621 ggcatagttg gtggctctaa taatgtatct agccaaattg aaaatcaact tgcagatatt
     1681 gataaagata gaaaagttca aagagttgaa ggagaaacaa gacacgatac taatgctaaa
     1741 gttatagaaa catattatgg caaattagat aaactatata tagcaaaaga tggatatgga
     1801 aataatggta tgctagtaga tgcattagca gcaggacctc tagcagcagg taaaggtcca
     1861 atacttctag ctaaaactga tataacagac tcacaaaaga atgcacttag taaaaaatta
     1921 aatcttggtg cagaagtaac tcaaataggt aatggagttg aattgacagt aatacagaag
     1981 atagctaaaa tattaggttg gtaataattt atactgtgga gaagattcct taattaatac
     2041 aggaatcttt ttctataatt atgctattta ttgtaaatta taacatttta taaagtagga
     2101 ggatgtagta gtggtagatg caactttatt ttttacacca catcaagatg atgaaacctt
     2161 aagtatggga agtgctataa ttgaacatgt tgaaaaaagt gatacacatg tcatattatg
     2221 cacagatggt agtaaatcta taataagaaa agttttggat gatggagggt catgttctta
     2281 tcatattaaa gatgttcata agtactcttt atcagaaagt gaattttcaa aagatagaga
     2341 tgaagaattt aaggatagtt gtgaagcaat gggtgtaaaa gaaagcaata tacatataga
     2401 agataataga gcccatgatg gtgagttaag taaagaaaag gcaagggaaa taatacttaa
     2461 gtacttagaa gaatatccag atgcaaaagt aaaaactgtt actccgttta aagctagtgg
     2521 aatacatgaa gaccatagag cattaggaga agctgcacta gagttatata aagagggcaa
     2581 aataaaagat ttaagatttt atgtagaacc atatgactat aaggatttta aaaaagttaa
     2641 tccaaatgta gaggtatgga aggtattacc tagtcaagaa gagaaattat taagtgcaat
     2701 gaatgcatat aaaaaatgga atcctgaaag tggacattat gctataggat accattcagt
     2761 aaaatctcat tttgatgagc tagctacaaa taaaatacag tatgtacatg ctccataaaa
     2821 tattatgaaa gaattaggag gtaaaaatta atgaaaatat caaaaaagat agtgtccttg
     2881 ttaactataa catttttaac tattatgtta tatggaaata catctaatgc atctacaaaa
     2941 gatacattaa caggttctgg aagatgggaa acagcaataa aaataagtca agctggatgg
     3001 acaaagtctg aaagtgctgt attagtaaat gataattcaa tagcagatgc tttatcagct
     3061 actccatttg caaaggcaaa agatgcgccg atattattaa ctcaaagtaa taaattagat
     3121 gatagaacga aagtagaatt aaaaaggtta ggtgtaaaaa atgtatattt gataggagga
     3181 acaaatacat taggtattaa tgtagaaaag caattaaaag cggaaaatat aagttttgag
     3241 agaatatcag gaaataatcg atatcaaact tctttaaagt tagctgagaa attaagtaaa
     3301 gaaaaggaaa tttctaatat agttgtagtt aatggagaaa aaggtttagc agatgcagta
     3361 agtgtaggtt ctgtagctgc gcaagaaaat atgccgatta tactttctga tacaataaat
     3421 gggataaaag atacagaaaa atttataaaa gaaaaaaata taaaaaaatc atatatagta
     3481 ggggggatat attctgtttc taattctgta gaacaaagtc tccctaattc taaaagaata
     3541 gctggaaata atagaaacga aacaaatgca aaagttatag aagagtttta taaaaataca
     3601 gatttgaaaa atgtatatat aactaaagat ggtatgaaaa agcaaagtga cttaatagac
     3661 tctttggcag taggagtttt ggcagctaag aataattcgc cagttgtttt agtaggaaac
     3721 aaacttgact ttactcagaa tgatgtatta aatactaaaa ttatcaaaaa tgttgttcaa
     3781 gttggtggat taggaaatga aaatgctgtg gaaagtataa ttgattcgca agaagaaact
     3841 acatacacag ttaaaaatac tgatgaatta aatgtagcca tcaaaaaatc agatgctaat
     3901 gatattataa agtttaagcc tgaaaaagaa aaaaatatta ctgaatcttt taaagtagaa
     3961 acagacaaat ctataagtgt agagtttgaa gggacatata ctcaaactat aagtataaat
     4021 atgccaaatg gtagcataac taatcttgga gatgtaaatg gtaccataaa aataatggat
     4081 gtaagatcta gtacattagt taataagggt gaaataaatt atcttgatat ttatgataat
     4141 aatggatgca gagttgaaaa tgaaagagat ggagatatat ggttagtaac tatatctaat
     4201 gaatccagag atgtgcatat agaaaatgat ggagatataa ataaaatatc taatagttgt
     4261 tctagtgtaa caataaaaaa ttcaggaaat attgatactt tgagtggaag taaagagata
     4321 gcgataagtg gaaaaaaacc aaaagttgat gatacagata aagatgatga aagagctagt
     4381 ggtataaata ttaaagttga gccttgttct atacctaaga gagattatgt aaaagttaga
     4441 atagtcactg agcctaaaaa ttcaaattat aaaatctact ataaagtagt gtccaaaaaa
     4501 cccacatcta gatatgttgg agataagata aaagtaagtg aatgggatga tgcaccttct
     4561 gcaggaacat catttgaagt tgaagcaaga aatgggtttt atatagaagc tgttgaaata
     4621 aatacttcga caaataaaat ttctaaatgg ggtaggtcag attcaacaga tgatggtttt
     4681 aaatcaatag aggtggcaaa aggtttaagt gcaaatgtat ttatagatgg tgataaggtt
     4741 gaactaacaa caacaagagc aaattctgga tgtgaaattt actatagaat tatgtctagt
     4801 aaaccaacag caatgaatgt tggaagtagt attactttat ctagttggga aaaaattagt
     4861 tccagtagtt tagaattaga gtcttctgaa gtaagtgaaa aatatattga attagtagaa
     4921 ctagaaaaat ctactaattt agtaactaga tggggttcaa ctgaaaaaat aaatatacca
     4981 aattctagta gataaaaaaa taaagtagtc taacatattt gtaggctact ttatttttct
     5041 ttaaaaatta tcattttact aaacaaataa tttaaaacta taacaattat attagtaaag
     5101 actttcacta taacatcatt tacaaaaagt aaatcaatca ttacatacat aattaacatt
     5161 tcaacgacac cagaaagtag cctaaaacta acaaaagaca aaaattcctt aaataagaac
     5221 tttatttcaa ttcttttact ttcaaacaca aaaaacttat tagtaacata agcaaataaa
     5281 acagcaagta tccatgccaa agcatttgca accataaaat taaataaaat aactctagta
     5341 aaaaataaat acgaaactat atttacaaga gtagtaaatg ctccaaaaaa taaatataat
     5401 attgtctctc tatgtttttt taaaatcaaa tataatcctc ctaagctaat ttaatataaa
     5461 tatacttaaa gatactaaca aacattagta tagcacatct attaataaat caatatatct
     5521 taaaatatta taaaaataaa actataatat tctataatta atatatactc ataatccata
     5581 ttttatcaaa ataaaatatg accttaattt agaaatctga tataataaaa attaaataca
     5641 tataaggggg taaacatgag aaagtacaaa tcaaaaaaat tgtcaaagct actagcattg
     5701 ttgacagttt gctttttaat agtgtcaaca atacctgtct cagcagaaaa ccataaaact
     5761 ctagatggag tagaaactgc agagtattct gaaagctatc ttcaatacct agaagatgtc
     5821 aaaaatggag acacagcaaa atataatgga gtaatacctt ttccacatga aatggaaggt
     5881 acaacacttc gaaataaggg tagaagtagt cttccatcag catataaatc aagtgtagca
     5941 tacaacccaa tggatcttgg tcttactaca ccagcaaaaa atcaaggaag tcttaataca
     6001 tgctggtctt tttcaggtat gtcaacttta gaagcatatc ttaaattaaa aggatatgga
     6061 acgtatgact tatcagaaga acatttaaga tggtgggcaa ctggtggaaa atatggatgg
     6121 aacttagatg atatgtcagg gagttcaaat gtaacagcta taggatatct gacagcatgg
     6181 gcaggtccta agttagaaaa ggacatacca tataatctta aatctgaggc acaaggtgca
     6241 actaaacctt caaatatgga tactgcacct actcaattta atgtaacaga tgttgttcgt
     6301 cttaataaag ataaggaaac tgtaaagaat gctataatgc aatatggttc agtgacatca
     6361 ggttatgcac attattcaac atattttaat aaagatgaaa cagcatataa ctgtacaaat
     6421 aagagagctc cattaaatca cgctgtagcg atagtaggat gggatgataa ttattcaaaa
     6481 gataattttg catctgatgt aaaaccagaa tcaaatggag catggttagt aaaaagtagt
     6541 tggggagagt ttaattctat gaaaggattc ttctggattt cttatgaaga taaaactcta
     6601 ctaacagata cagataacta cgcaatgaaa tcagtatcaa aaccagatag tgataaaaaa
     6661 atgtaccaac ttgaatatgc tggtcttagc aagataatgt caaataaagt aacagcagca
     6721 aatgtatttg attttagcag agattctgaa aaacttgact ctgttatgtt tgaaacagat
     6781 tctgtaggag caaaatatga agtatattat gcaccagtag taaatggagt tcctcaaaac
     6841 aattcaatga caaaacttgc aagtggaaca gtatcatatt ctggatacat aaatgtacct
     6901 actaattctt acagcttacc aaaaggtaaa ggagcaatag tagtagttat agacaacaca
     6961 gcaaatccta atagagaaaa atcaacttta gcatatgaaa ctaatataga tgcatattat
     7021 ttatatgagg ctaaagcaaa cttaggtgaa agctacatac ttcaaaacaa taaatttgaa
     7081 gatataaata catatagtga attttctcct tgtaactttg ttataaaagc tataacaaaa
     7141 acatcttctg gacaagctac ttcaggagaa tctttaactg gagcagatag atatgaaaca
     7201 gcagttaaag ttagtcaaaa aggatggact tcttcacaaa atgcagtatt agtaaatgga
     7261 gacgcaatag tggatgcttt aacagctaca ccatttacag cagcaatcga ctctccaatt
     7321 cttttgacag gaaaagataa cttagattca aaaactaagg cagagttaca aagattagga
     7381 actaaaaaag tttatctaat aggtggagaa aattctttaa gcaagaatgt acaaactcaa
     7441 cttagtaata tgggtatatc agtagaaaga atttcaggta gtgatagata caagaccagt
     7501 atatctctag ctcaaaagtt aaatagtata aaatctgttt cacaagttgc agtggcaaat
     7561 ggtgtaaatg gacttgcaga tgcaataagt gttggtgcag cagctgctga taacaatatg
     7621 ccaataatac ttactaatga aaaaagtgag ttgcaaggtg ctgatgagtt tttaaattca
     7681 tcaaaaataa ctaagtctta tataattggt ggaacagcta ctttatcatc aaatttagaa
     7741 agtaagcttt caaatccaac aagacttgca ggaagtaata gaaatgaaac taatgctaag
     7801 ataatagata aattctaccc tagttcagat ttgaaatatg cttttgttgt taaagatggt
     7861 tcaaagagtc aaggagactt gatagatgga ctagcagtag gagcattggg tgctaaaact
     7921 gattcaccag tagttttagt tggaaataag ttagatgaaa gtcaaaaaaa tgtacttaag
     7981 tctaagaaaa tagaaactcc tattagagtt ggtggaaatg gaaatgaaaa tgcttttaat
     8041 gaactaaata ctcttttagg aaaatagtga ctggaaagat agtaaggata tacattttat
     8101 tattaaaaaa tattcaatag aagtttataa atataaaata acaaaaaggg gtgtctcaaa
     8161 atgaactttt tagttcatga ggcactcttt tttgttgttt tatattttta tcatttaaat
     8221 aatgaaaaat aggattgtct atatttttag atagttttct tattattgat gaaaaatgta
     8281 ataattttaa actgtaaaat agtgtaaagt tattttataa tataaatata gtaagatttt
     8341 aaacaaaata agggggaata ataatgaaag caccaaaaac tattttaaca atactgacaa
     8401 tagcgcttac tttaagtagc atttctataa taccatcata tgcacttaca gaggaaaaat
     8461 taataggtaa tggtagatat gaaactgctg taaagataag tcaaaaggca tacagttcta
     8521 gttctaatgt agtgttagtt aatgacaatt cattagctga tgcactttca gccacaccat
     8581 ttgcaaaggc taaaggagca ccaatacttt tgacagaaag tgataagcta gatgatagga
     8641 ctgaaaaaga gataaaacgt ctaggagcta aagatattta tttgatagga ggtactgctg
     8701 tacttaataa agatatagag aacaagttaa aaggtaatgg acttaatgta gaaagaatta
     8761 atggtaagga tagatatgaa acttcactga ttttggctaa taagcttaag gatataaaag
     8821 atattaagga agttgctgta gtaaatggtg aaaaaggatt gagtgatgct gttagtgttg
     8881 gagcaccagc agctcaaaat aagatgccta taattttgtc tagtcctaag gatggagtag
     8941 aagcatttga taaatttata agggatgaga aagttataaa agcatatgta attggaggta
     9001 caacttcagt ttcaagagct gtagaaaaga gtcttcctaa tgcagaaaga gtgagtggga
     9061 aagatagaaa tgagactaat gctaaagtta tagaaaagtt ttacactgat acaaatttaa
     9121 gtaatttata tgtaactaag gatggtagta aaaatgagaa tcaactaata gactcgttag
     9181 ctgtcggagt attggctgca aaaaatgagt ctcctgttgt tttggttggt aataaattaa
     9241 acacaaaaca aagagatata cttagtacta aaaaattaaa tactataaca caggtaggtg
     9301 gaaatggaaa tgaagaagct tttgatgaaa ttaaaagtct acaagaaaaa actgtgtttg
     9361 aagcaaaaac tgttgaggaa ttaactgata tgataaatat agcaagtcct aatgatatta
     9421 taaactttaa accaaaagag aatactgtaa atgaagcttt tagaatggta acaaataaac
     9481 caataactgt gaatataaag ggtgattgtt caaagactct tacagtagat atgcctaatg
     9541 gtgaagtgaa taactatgct actttggtaa atgttatagt tagaaatatt ggagaaggtg
     9601 gatttaataa tcatgataca atcactatat tgtcagttcg tgacaaaaat ggtagagtta
     9661 tagaaaatac tagaaattca gatatagata cgttgatgat attagcttct gctaatgata
     9721 caaaattgat taatgatgga tatataggaa aacttataga taattcatct aatagtgata
     9781 taactaataa tggtactata gataaaaaag ttaatcaggt tgaagattta gaggctaaag
     9841 tagattcaat tgaaaaagct atagattcta taagtcaaaa agtcaataaa atacaagata
     9901 tactagataa gcttggattt ctaaagaaat ttcttagcta agaatatgta tatttaagtg
     9961 aagtgtgtgt tgtaattgtt ctaaatttat gtagttaaac aggtttatat acaaatagat
    10021 ttatagataa atatgtagtt tagtatatat ttgaataagc tttggggagg taacatgggg
    10081 attggaattg aatgtaacaa aaaaataaat aaaagtattg acaaaactag aataaagaaa
    10141 aataatct
//
LOCUS       DAFKMX010000144        10117 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFKMX010000144 DAFKMX010000000
VERSION     DAFKMX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.144"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..1053)
                     /locus_tag="LZB08_002822"
     CDS             complement(<1..1053)
                     /locus_tag="LZB08_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBM7316310.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNID"
     gene            complement(1426..1941)
                     /locus_tag="LZB08_002823"
     CDS             complement(1426..1941)
                     /locus_tag="LZB08_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBM7316311.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2304..3374)
                     /gene="leuB"
                     /locus_tag="LZB08_002824"
     CDS             complement(2304..3374)
                     /gene="leuB"
                     /locus_tag="LZB08_002824"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBM7316312.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(3381..3872)
                     /gene="leuD"
                     /locus_tag="LZB08_002825"
     CDS             complement(3381..3872)
                     /gene="leuD"
                     /locus_tag="LZB08_002825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBM7316313.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3887..5164)
                     /gene="leuC"
                     /locus_tag="LZB08_002826"
     CDS             complement(3887..5164)
                     /gene="leuC"
                     /locus_tag="LZB08_002826"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBM7316314.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5121..6863)
                     /gene="leuA"
                     /locus_tag="LZB08_002827"
     CDS             complement(5121..6863)
                     /gene="leuA"
                     /locus_tag="LZB08_002827"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBM7316315.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISKTC"
     misc_binding    complement(6987..7233)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7381..7827)
                     /locus_tag="LZB08_002828"
     CDS             complement(7381..7827)
                     /locus_tag="LZB08_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316316.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7848..8567)
                     /locus_tag="LZB08_002829"
     CDS             complement(7848..8567)
                     /locus_tag="LZB08_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBM7316317.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPRKVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8685..9959)
                     /locus_tag="LZB08_002830"
     CDS             complement(8685..9959)
                     /locus_tag="LZB08_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBM7316318.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3058 a         1955 c         1068 g         4036 t
ORIGIN      
        1 atcaatatta tctaataatt cttgtacatc ttcgacagta tagtcaccca tatgtgaaat
       61 tctaaaagtt ttttctttta aatctccata gccatttgct atttggaatc ctcgttcttg
      121 taattttgaa tttaaatttg atacactaat accttgagta ttttctataa cagtaagtgt
      181 attggataag tgattttcat tggtaaaaat ttggaagtgt tttttagccc agtttctaac
      241 taagtttgcc atgtcttcat gtctgttgaa tctgttatct aggccttcat ctaatatatt
      301 atctaattgg aaatctaaag cgaacatgtg agaaagagat ggtgttgaag ggtattggta
      361 gtttttcttg attaagtatt catacattgc taacaaatca agatatactc ctctaaaagg
      421 tacttgctta gctctatcga ttgctttttg agaaaatgtg caaatagaca ttcctggtgg
      481 taatcccagt gctttttgta ctgatgttat acaaatatct attccaatct tatcaacctc
      541 gacttttatt ccaccggctg aacttacagt atctacacag tagattacat cttcatactt
      601 tttaacaact tctccaattt cttctattgg atttcttatt ccagttgaag tttcattgtg
      661 agttacagtt attaaatcgt attctccggt agaaagcacc ttatcaacca tttctggcgt
      721 tgttgcctct cctaattcaa ctttaaatat atccgctggg acattattag ccactgccat
      781 tttgtaccat ctatcaccaa aggatccaca ggaaaatact gctgcctttt tggatgtaca
      841 ggaacgaatg gagccttcca ttaaaccagt tccggatgac gtggataaaa gtatcgtgtt
      901 gtttgtatag aatagttttt gcatcttttc acttatactc ttttggagca tagatgcatc
      961 ttttcctcta tgtcctatca ttggtgtagc catcttttgc aagacttctt ctttaacatc
     1021 aataggacca ggaataaata atttcttact cattataatt cccccctttt tagtttgtaa
     1081 tatggatagc gtttttactg tccttatttt taataactat aatatcaagc tgaattaact
     1141 atgtcaacat aaaatttatt ttttatatct aatttatttt atatatttta tgaaaacatt
     1201 cattttctac cttttcataa gtctgaattt tctgaattat tctctcaaaa aaaatatact
     1261 ttttatctat ataaattaat ttttttagta tgtaaatttg acgctattac gataaatctt
     1321 tttagtataa ctaatttatt tttatcttgt atttttattg tatttttgaa ttttattttt
     1381 tctaaatata aaatttatgt tttaaaatgt aaaattttta tttaatcaaa gttcaatata
     1441 ttccactttt tctttcgatt cttcaaaaaa ccattcttca ttagaaaaat caccttctgc
     1501 aatttgtcca ataggcattt tcatatactt tccagttgct gtcactgcta tatctccatt
     1561 tggtagaatt atttctcctg taccttcaaa taattttctg ttttctttag ttattcttcc
     1621 aacaactttt atagtagcat ctgttggtac tggtttctta tatttcaact ctaaagatac
     1681 tgttactccc catatttggt catcatttat acttacagct cttccaatag tctcatccaa
     1741 tattgcagca gacatacctc catgaactct cccaggataa ctttgatgcc aatcctttgt
     1801 attacaaatt gatacaagct ctccattttc aagttcataa aaatctgctt taagtcctag
     1861 ctcattttgt gttccacaaa ctaaacaaga tttactacta ttttgcttct tcaaaacttt
     1921 atactttgcc aaaaaatcca tctcctaacc gacattagta gtcgcttaat attaaataat
     1981 tctaaaatat attatataat attaataaaa tccctgcaaa cttctaataa taaaaatagg
     2041 ctacttcaat atgataagtt taatttaaag tattattttc caaaaaataa ctttatcaaa
     2101 atagaaactc aaagtagata taaacctctt tttcgttatt aaaatagtaa aaatatattt
     2161 gacttaatat aaaaaagagt gtctcattaa ctaataaagt ttattttgag acacccctca
     2221 taaaacaaat tatactacaa tagattttta ctaattctac aagttatata ttcaaattat
     2281 attattcaaa ttaaactata attctaaatt tcatttacta ttaattcgcc catttccctt
     2341 gttcctacaa catttcccac accattatag atatcaatag ttctataacc tttattgagt
     2401 acactttgta cagcatcttc aatacactta gcttcttctt ctaagttaaa actatgtctt
     2461 aacatcatgc ttacagatag aatagtcgca attggattta caatatcttt tccagctata
     2521 tcaggtgcac ttccatgtat tggttcatac ataccaaaac tatcttctct aagtgatgct
     2581 gacggtaaca tacctataga acccgtaatc atagatgctt catcagatat tatatctcca
     2641 aatatattat tagtcactat tacatcaaat tggctagggt cttttacaac ttgcatagca
     2701 gcattatcaa catacatatg tgacagttct acatcttcaa actctttagc taagtcttcc
     2761 attatacttc tccaaagtct tgaactctca agcacatttg ccttatctac acttataagt
     2821 tttttatttc tatttctggc tatttcaaaa gctctcttac caattcttct tatttcattt
     2881 tcgttatata tttctacatc ataagcattt tcgataccat caatgatttt tcttccacgt
     2941 tctccaaaat aaatgccccc tgttaattcc cttacaacaa caaaatcaat gcctttctca
     3001 actatatccg ttctaagtgg tgaagcttct tttatacttt catacattgt agctggtctt
     3061 aaatttgcaa ataaacccaa tgactctctt aattttaaaa gaccagactc tggtctttta
     3121 tctcccttac atttatccca ctttggtcct ccaacagaac caagcaatac agcttcgttt
     3181 ttccgacaag tttcaacagt tttattaggt agaggttctc cagtttcatc aatcgcgcat
     3241 cctccagcta atacatattc acaatcgaat ctatgattaa atttacaaca aattttattt
     3301 agaaccttta taccttcatc tataatttcc ggtccaacac catcaccttt tatgactgct
     3361 atattacaat tcatactcca ctacccccta tttgctttta ttgagttaat taagccttca
     3421 tttaatataa tattttgcat aaactcagga aatggttctg ctttatattc ttttcctttt
     3481 gttatatttt ttatcacacc tgtagaaaaa tctacttcta tattatcccc ttcatctatg
     3541 ttattggcag cttcttcaca ttcaagtata ggaagcccta tatttataga gtttctaaaa
     3601 aatattctag caaaagtaga agcaatcaca caggatactc cactttcttt tattacaatt
     3661 ggtgcatgtt ctcttgatga accacaacca aagtttttag ttgcaactat aatatctcct
     3721 ttttttacct tagaaataaa tttatcatca atatcctcca tacaatgcgt tgctagttct
     3781 ttataatcag ctatatttaa atatctagct ggtattatta catctgtatc tatattgtca
     3841 ccgaatttaa aaacacttcc attggcaatc ataaatttta cctcccttaa attatttctg
     3901 atggttttga tatttttcca gttatagctg atgctgctgc tacagctggg cttgcaagat
     3961 acacctctga atcaacatgc cccattcttc ctacgaaatt cctatttgtt gttgaaatgg
     4021 atttttctcc tgctgcaagt atacccatat gtccccctag acatggacca caagttggtg
     4081 tagaaactgc tgctcctgcc tctataaaag tggtaatata ccctttttct atagcttcaa
     4141 gatatacttt ttgagttcca ggaaatatca taactctaac tccatctgct acttttttgc
     4201 ctttcattat ctcagctact acttcaaggt ctgatattct tccatttgta caagagccta
     4261 ttacaacttg gtctatcttt atatccttat ctatctcatc tactgttcta gtattttcag
     4321 gaagatgagg aaaggctact gtttctttta gtttggctaa atctatttcg tatacttcat
     4381 catagactgc atcttcatct gcttcatata tatttacatc ttttgtcata gttgaacact
     4441 tatgtgaatt tatatattcc atagtcttat cgtctactgg aaaaattcca ttttttgctc
     4501 ctgcttctat tgccatatta caaatagtaa atctatcatc catagataag tattctactc
     4561 catctccaca aaattccatt gacttgtaaa gtgctccatc aacacctatc tcccctatta
     4621 tatgaagtat tatatcttta ccacttatcc atttatttgg tttatttttc aaaacaaatt
     4681 ttattgctcc aggaacctta aaccaacatt ttcctgtagc catacctgct cccatatcag
     4741 ttgaacctac accagtagaa aatgctccta atgctccata tgtgcaagta tgagagtcag
     4801 cacctataat tacatctcca gcagttacta tacctttttc aggaagaaga acatgctcta
     4861 ttcccatatc tcctacatca aaaaaattta caactccttt tgattttgcg aaacttcttg
     4921 taaatttaca ttgttctgca ctctttatat ctttattagg tgtaaagtgg tctaaaacca
     4981 tcactacttt agtcttatca taaacatctt caattcctaa tttttcaaat tctctaatgg
     5041 ctactggtga tgttatatca ttacctaaaa ctacatctaa atctgcttcg attaattgcc
     5101 ctttcttaac ttcatttaat ttagcatgtt ttgctaatat tttctgcgtc atagtcatcc
     5161 ccatttttta tttcctcctc aatataactt ttatttattg cattcataag tgcattaata
     5221 gctgctgtat ttatgttttc cgatatactt acaccaaaat agacttcatt attgtgctct
     5281 attcctacat aagctacccc tcttgaatgt gacccttcct ctaatgcatg ttcccagtaa
     5341 tatctaaatt tataatcttt catgccacat tgctttagtg cattattaaa tgaatctaca
     5401 ggtccatttc ctataccaac aatcctttgt tcatttccca tataacttat tcttgcagtc
     5461 atctcaatca tgttagtatc attagaatca tcattttcat agtttaattc atccattgat
     5521 tttattttat acttcttaac tttataaggg ctctcaactt caatatattc tttcttaaac
     5581 aaattaaata ttgcttcact actaagttct tccccaattc tatcagattc cattttgact
     5641 acatttccaa aatcagaatg catattctta ggtatcataa atccataatc tgtttccatt
     5701 atataaacag ctcctccttt tccagattgg ctatttatcc ttataatttc tttgtattct
     5761 cttccaatat catgagggtc tattggtaaa tatggaactt cccagtaatc attatctctg
     5821 tttttcattg ctatcatgcc ttttctaata gcatcttgat gtgaacctga aaaagcacaa
     5881 tgtacaagat tacctgcata tggatgtcta tcatgtacca taagttttgt acagtcttca
     5941 tatataccta ttatcttatc tatattgcta aaatctagct ctgggtctat tccttgtgta
     6001 taaagattaa gtcccacatt tactatatcc atattcccag ttctttcacc atttccaaat
     6061 agtgttcctt caagtctatc ggcaccagcc aataatccaa gttcacttgc agccgtacaa
     6121 gttcctctat cattgtgagt atgcaaactt agtataatag aatccctaca acttatattt
     6181 ttacaaaacc attctatttg gtctgcatat acgtttggtg ttgccatttc aactgttgat
     6241 ggaagattta ttattacttt tttttgagga gttggtttcc aaacatctat cactgcctca
     6301 catatttcaa gtgcataatc catctctgtt cctgtaaaac tctctggtga atattcaaaa
     6361 gtaaattctg tttctggata tttttttact tcttcattaa ataattttgc tccttcaact
     6421 gctatatcta ttatttcttg tttactcata ttaaaaacca catctctttg aagaacagat
     6481 gtagagttat ataaatgtat aattgctttt ttacatcccc ttattgattc aaatgttttt
     6541 tctataagat gagccctaga ttgtgtaagt acttgtatag ttacatcatc tggaatcata
     6601 ttctcatcta ttaattttct taaaaatgta tactctgtat ttgatgctga tggaaatcct
     6661 acctctattt ctttaaatcc tgtatcaata agcattttga acattctgac tttttcattt
     6721 acactcatag gtgttggcag tgactgattt ccgtctctta aatcgacact acaccaaatt
     6781 ggagccttaa atatttcatt atctggccat ttacgattta ctatttgcat tttatcatat
     6841 tttttatact ttccacactt catgttattt cccccttttt ctgttttttg tattcagtat
     6901 acctcatatc gacgcaatcc tgcacaattc ataaggataa atgtcaattt tatcattaga
     6961 ataaaaaaaa cctcatctct aataaaatta gagacgaggt tttgctcgcg gtaccactct
     7021 atttagcttt ctaaaaagct cccttagtaa tagccaatac aataattata atgactatat
     7081 ttcctataac gtggaattac tccgattatt ctaagattta aatcttagaa aacagctcct
     7141 agacaagttc agaactatca actactaaat cacaccattc tttagctctc tgaaagtttc
     7201 attgttctta ctacttctat tcatcacttt tttaatcatt taaattttct aaatattttg
     7261 atttaatagt tattatatac acattaaaat gtattgtcaa cacaaaaaat aattatctca
     7321 attttttatt tttgataagc aattctataa aacatgctaa ctattgaaaa cagtatgata
     7381 ttatgcctta aactttttca tcttttcatc tatgatattc aataattttt tttcatcttc
     7441 caaactcaat gcccttaatc taattttata attagattca tcagtgtata tatttaatat
     7501 attttttact acttctattt tttctattat cttataatta aacttttgac tttgtttaga
     7561 ttttatactg aatatacatt gattatttat taatattgaa tgattatcga gagtaatata
     7621 tacttttccc tcatatttat ttgaaaaatc acgaccttgt ttaaatagaa aaactgctgt
     7681 tattatacat aaaatatata atattgtcgt tgaatatata aaaattgtag ctaaaattat
     7741 aagtgctaaa accatatata tagtcattcc ttttttaaaa taaccattgt cataataatt
     7801 catatagata aacttatttt tactcaaatt ttttcactcc caagattcta aaaatttata
     7861 tcaaaaaatt ctataatttc atcttcatga taatttatga ctaattcatc tggaaaatct
     7921 actgcttcta aaactttttc tgcattttca aattctccta tagaataaca tatatgagag
     7981 tcagtcccaa gaattactct tgcaccatat gttttacaca atgtcaacat atgacttaca
     8041 ttttcccaag tacccgttct atgagagttt gaacttaatg atgaattatt tatttctaaa
     8101 aggatatttt tatctttagc tttttttact ataggttcat aatctaaagg atacctactg
     8161 tcatctggat gtccaattat ttttacctta ggtttatcca taacttttaa aatagcattt
     8221 gtattttctt cttttgtacc agaagctata caaggtctat gcaaacttgc gattatataa
     8281 tccaaatgtt gcaacatatc ttcttgaaca tctaaattac cattataatc cattatattt
     8341 ccttctattc ccctaagaat ctttacgcct tgcactttac gtggaagcaa atgcaaatta
     8401 taaaaataga atggatgtgg tcctcctggc atattaggtc cgtggtctga tattcctaag
     8461 tattttatat tcttcttact cgcctcttcg acattttcct tgaaagtact atgtgcatgt
     8521 ccacttatta gagtgtgaca atgtacatcc ataatagact ttttcattaa atttcctcct
     8581 aatattttac ataaattcgt gaaaatagct aaagggctat ttcaaaacta aagctgtcat
     8641 aatagacagt gtaacgtttt aaaacagccc cattttcaat tattttacac ttctaaatct
     8701 ttaaatttat ctttaaataa ttctccaata cttcctaaag attcctctac ttctaggtat
     8761 tctgttatat cctcttctgg ctctctagtt gcttctttta ttgaaaccaa cattctttta
     8821 gtctttggtt taaaatcaag aatcttaact ttaacctcat ctccaatatt aacaacatta
     8881 gtaactttag ccactctagt atcactcaat tcagatattg gtaaatacgc atctacacct
     8941 tctttaattt gaacaaaagc ccctttttct attattctaa gaactttagc attgacaaca
     9001 tcatcaattt gtacttcttt tgctattaaa tcccaagggt cattatttat gtcttttaat
     9061 gccaaggcaa tttttttagt ctctttattt acatccaaca cataaacttc tacttcttgt
     9121 ccctctgaaa caacatcttc aacactttca actcttgtcc aagctaaatt atttatatgt
     9181 gctaatcctt caatcccacc aatatccaca aatgcaccat aaggcattat ctttgtcacc
     9241 ttaccatctc ttttttgtcc aacttcaaga gaattaaata agtcttcttt agctgctttg
     9301 atacgagctt tttcttcttc tctttcttgc ttaattcttg ctctttcagc ttctttttct
     9361 gccttaattt gagctcttct ttcttctctc tctttattta tacgctcttg taatacatct
     9421 ctatgagatg ctacaagtct attttttcta acatccaatt cttttatata aacttctaag
     9481 ttaagaccaa caaatttaga tgtatctgta ataaattttg tatcaagttg agaaattggt
     9541 ataaaaaatg tatatgtatt gtaattagca aatacacctt tatctatatt cttttcaaca
     9601 tttacagtta ttattctatg ttcatcataa gccttttgta actcggcaaa atcatttctt
     9661 ttgtcaagtt gtaatttaga taaagttatt gtaccatctt tttgagatac ttttgttatt
     9721 acagcagtta tttcatcacc tatgtggtat atatcttcta tatattgatt tttttctatg
     9781 ttcaattcac ttataggtat tattccatca aacccatagt ctaatcctaa ttgtacttca
     9841 tcatttctca ctgcagttat ttttccagtt gtcaattctc caactttcac tttgctgaat
     9901 acttgttctt gctggtcaag taattcttgc attgttaaat cattttccat tataaacatc
     9961 ctttcctctt attgattatc atatctgtaa atattatacc attaatattg caaatactta
    10021 catttttctt actataattt ttaaaataaa ttcaacttca tatatttata cattatagaa
    10081 aatatcttat taaattaaaa tacaaattct aattttt
//
LOCUS       DAFKMX010000145        10112 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFKMX010000145 DAFKMX010000000
VERSION     DAFKMX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.145"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..541
                     /locus_tag="LZB08_002831"
     CDS             <1..541
                     /locus_tag="LZB08_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316319.1"
                     /translation="KELLKKIELIDIIDNYNFSREPVNTKNLGKKMIDNGNLSNLKEL
                     LLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWIVDGGEEYKMKNYMESNLIYSRV
                     GKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQ
                     SSELELNTNVGLFIQEIMK"
     gene            561..1574
                     /locus_tag="LZB08_002832"
     CDS             561..1574
                     /locus_tag="LZB08_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBM7316320.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINGRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            1590..2387
                     /gene="cas5"
                     /locus_tag="LZB08_002833"
     CDS             1590..2387
                     /gene="cas5"
                     /locus_tag="LZB08_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBM7316321.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            2414..4822
                     /locus_tag="LZB08_002834"
     CDS             2414..4822
                     /locus_tag="LZB08_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBM7316322.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            5263..6210
                     /gene="prmA"
                     /locus_tag="LZB08_002835"
     CDS             5263..6210
                     /gene="prmA"
                     /locus_tag="LZB08_002835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBM7316323.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            6229..6987
                     /locus_tag="LZB08_002836"
     CDS             6229..6987
                     /locus_tag="LZB08_002836"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBM7316324.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            6989..8287
                     /gene="mtaB"
                     /locus_tag="LZB08_002837"
     CDS             6989..8287
                     /gene="mtaB"
                     /locus_tag="LZB08_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBM7316325.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKNINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            8330..8680
                     /locus_tag="LZB08_002838"
     CDS             8330..8680
                     /locus_tag="LZB08_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBM7316326.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            8843..9022
                     /locus_tag="LZB08_002839"
     CDS             8843..9022
                     /locus_tag="LZB08_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBM7316327.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            9052..9495
                     /locus_tag="LZB08_002840"
     CDS             9052..9495
                     /locus_tag="LZB08_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBM7316328.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            9606..10103
                     /locus_tag="LZB08_002841"
     CDS             9606..10103
                     /locus_tag="LZB08_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBM7316329.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
BASE COUNT         4243 a          925 c         1878 g         3066 t
ORIGIN      
        1 taaagaatta ttaaaaaaaa ttgaacttat agatattata gataattata atttctcaag
       61 ggagccagta aacactaaaa atttaggtaa aaaaatgatt gataatggga atttatcaaa
      121 tttaaaagaa ttattacttg ataatataag ttttttaaag caatcaactg gaacttctcg
      181 agaaatggat tttgtatcta gctcatataa tatcggttta tatataaaat tgtgttggat
      241 tgtagatgga ggggaggagt ataaaatgaa aaactatatg gaaagtaatt taatatattc
      301 aagagtaggt aaagacttat ttaataaaat gacagatgaa tccagaagaa aaaatttttc
      361 tatgaaagtg attcaactac ttaagtctaa tgatagaact cagctatttc aaactataat
      421 gcatgtgtta gtttctaatg gaatcttaat aggagaaggg tttgtagaag gtctaatgca
      481 gagttctgaa ttagaattaa atacgaatgt aggtctattt attcaagaaa tcatgaaata
      541 aaaaaggggg aaaatttaaa atgaatgaaa aaagagtaaa aggagtaaca ttaactatac
      601 tgacagaaag cccagttcca ctttcatatg accaagggta tggaaattat acaccaataa
      661 aaaaagaaca atatagagaa aaaatacatg caaaaactag tatagccaca ataacatatg
      721 atttaagaag aatgctccac caagaatatg gttggaattt aagtaatatt gtttttggaa
      781 aaaaaggaaa tatatatcca tctattaaga aaaatgtagg atgtgtagat gaaaatggat
      841 tagagactga tgtatttggt tatttgatac cacttaaaga taaaggttct ataagtaaag
      901 cttctccact tagaataata ccttttaggt cattaaatcc atatataggt tcaacacagc
      961 taataacaaa tagaggtttt ttaagtagcg aatttggtag aaaatattat gatgagaagg
     1021 aagaaaatga agtacctaga gatgaaaact ttccaacaac acaagcatta gcaatagaag
     1081 agactttatc agattactac gtttatacaa taacattgga gcttgataga ataggagtag
     1141 tagaagtaga agatgggaag ttacttttac cagaagaaag aaaatttatg agtaaggaat
     1201 taagagaaaa ggcagtaaaa gatatattag atgcaattac tgtgtttaca agaaacataa
     1261 aacattcatc agtactatta aaaccacttg cagtaatagg tggagctttt gataaagttg
     1321 taccattttt ttgggatgat gtagattata atgctgatag tggtgagata aatttagagg
     1381 gagtcataga aactatagaa agttatagcc ttaaagaaag taacacaata cttgctatca
     1441 atggcagatt aaaaatttct aataaaaagg aattaaataa gtttaattta agtaaatacc
     1501 ctgtaaaaga gattaaaaat ctagcagata gattggaaat tggagaagat aatatgtggt
     1561 atttaaaaga gtaggaggtt ttttgaatta tgaaagtatt acaatgtaaa cttaagcaac
     1621 caactgctca ctatagagac ccaaaagtat ttcaaaatga atatataagt actttaaatt
     1681 taccatctaa gaccacaata atgggaatga taacgtattt atgtgataga agattaaact
     1741 ctgatataga tataggaata attggaacac atcatcatag agaactagaa ttttcaagag
     1801 gagagaacat tgatttttgg aatgaatata caaatatgag aaaaggcaaa gataaagaaa
     1861 agtttttgct tcaaggaaac tactatgatt actataaaga acataaagct caaaatagca
     1921 tattaaacta tgaagttctt aaagaagtag aactgactat atttatttct tgcaaggatg
     1981 atgaagaact tgaatttatt aaaaaaaagc ttgaaagtcc atgtaaatat gcaaatttag
     2041 gaagaaaaga agattttgta ataccaagtg aaaaagggtg ttttgtgaaa gaagttgagt
     2101 taaaagaggt tatacctatg aatactcgtg atgctataaa agaaaatata aaattaaaga
     2161 atacatatgt aagagttgac ttaagagata aagagaatgt cgagactata ataaatcaag
     2221 gtgtattgat tgcattacct cataaatata aagatttaga agcaaataga gatgatagag
     2281 tttatgagtt ttgtcattat atatatgttg ataatgatgg tatatatcct aaaaatatta
     2341 aggtaaatgt atgtattgat acaaaagaag tttttacatg gctttaaatt attttgaaag
     2401 ataggtgttt atcatgaaaa atatcatgca aaaagaaata ttagctaagt catacaattt
     2461 aagaactaat caacaagagc aaacattagt tgaacatatt aaggatttat ttgaagtatt
     2521 agaatctata ttagaactaa atctatattc agataaagat gtagagatat tgaaaatatg
     2581 ttgtgctttg cacgatttag gaaaaattaa ttcaattatg caacaaaaaa tggagattaa
     2641 taataaaata tcgtatagtt gctcagaaga agagaggaaa aagttagagt ctgacaaaaa
     2701 atctttaaaa aagatagcaa gacataatat attttctgga gcatttttaa aagatatatt
     2761 agaaaaaatg aacttgtcag aagaagataa gttatatata tataaaagta taatgttaca
     2821 tcatggaaac tatgaagatt atatgaggtt aagtattagt aaagttcaag aagaaattta
     2881 tgagtatatt gaaaaaggca ttttagaaaa agaagagttt aatctgaaag atatagaaag
     2941 ttatataaat aacattttaa atgtagactt taaatttggt gaagatgtat tggattatga
     3001 ttacatagat aaattaagtg aaagtatgtt tatagacagt gattataaca atggtcaaat
     3061 agatgattca gttttaaatt atagaaagtt taagtatatt ttatataaag ggatgctgaa
     3121 cttaatagac catagtgcat caagtagaca gaaaggtata aaattttata acgattttac
     3181 tgatgaagaa attgataata tgattttaaa tgagatatat aagagtcagg gtaatttaga
     3241 aaaaaataat atagagttca atactataca aaaaaggttg agagaacttt ctggaagaaa
     3301 tgttctgact gttgcattta caggttcagg aaaaacagct gcagattata gaaagacttt
     3361 taaaagaaaa ttctttttag tgccaaataa aatatcagca gaaagttttt atagaaaaaa
     3421 tatttttcaa aataaaaatg atttagatac aagaagtagt aatgattaca ttggattact
     3481 tcatggagat ataaacttat attcagagaa tgaagataat ggagaacatg attttgtatt
     3541 gacattaagg gatatagatt tgagtataaa cttttgtaaa ccttgtgtaa ttgcaacagt
     3601 tgaccagtta ttacttagta tgtttaaatt tccgggatat gaaaaaatat tcgcagccgt
     3661 aaaaaatgca tctataactg ttgatgaagt acatttattg gaaccaaaga tgtttttgat
     3721 tttgatatat tttatgcagt ttgcatgcaa gtacttagat gttgattttc acttaatgac
     3781 agctacactt ccaaaatcat ataaagagca aatgataaac aagggaatta tttttcaaga
     3841 agaaagtaat aaaaatgtta ctgataaagg agaaatagtt tttattgaga gcaacaagga
     3901 agaagagatt tgtgaaggta aagatgtaaa ggtttctttt ataaaggaaa aagaaatcaa
     3961 gtcaatagtt gaagatgctt tagaaaataa acaaaaaatt cttataataa agaatactat
     4021 tgattcagtc aatagaacat atgagttttt aaaagaaaat ttatctgata agtatggtgg
     4081 tgtagatata gatgttttac atagtagatt taagttcaaa gataaaaaag agaaatatag
     4141 taaaatatta aatggagagg gtgatatttg gatatcaact caatcagtag aaatatcctt
     4201 ggatttggat ttcaatataa taataagtga tttagcaact atggatagct tgatacaaag
     4261 aatgggtaga tgcaatagaa ataataaata tgaatatgga aacttttata ttctacctag
     4321 tgaagataaa atttatgata acaaacttaa gaacacaact aaaaacatct taaaggacat
     4381 attaaaaact aactctattt ttacaatggg aattagaaaa actattttgg atgattacta
     4441 tgataatagt gtagtcaaaa aatattttga agagaatttt ataagttgtg ataaagaaat
     4501 taagaatatt tatggtataa ataaagaaat ttttgatgga ttagatttaa tttttaattt
     4561 tgaaccttac aaaaatattg ttgatagtaa gaaggaagca gctaaaattt ttagagatat
     4621 tgatgttagt tacaaaatta ttctggaaga agatttttat aaggaagata gggagttaca
     4681 acaagattcg attcaagtat cagggtttat atttaataga ttatattatt atggattaat
     4741 aagtaaagta gaaggataca tggtactaaa atctagtagt aagtttgagt ataatagtac
     4801 tgtaggctta attttgaagt gagcatatgt ttaaatataa tataccttta gccattgaaa
     4861 atacaaaaag tactactgta agtccactgt aaaaactatt ttttaaaagg aaaaataagt
     4921 tgtgagatag agtgtttaca agtgtatata gataataaat ttgaggttta agattaacta
     4981 agtgggtttc aggtacatcg cgaaaataat tataagttgg agattttgta tgtttaagat
     5041 taactaagtg gatttcaaat attgatatag tagatataat agaagtttta ttattaggtt
     5101 taaaattaac taatcggatt tttaaatatt ctgactatta tataaggata ttattagctt
     5161 gaatttaaaa ccaataatat aaaacaacaa aatattatat ttatgatata ataaaagtta
     5221 tggaaaaaca ggaatattac tatagagaag agaggtaaac ttatgaataa ttggatagaa
     5281 gtaactataa aaacaacaac agaagcagtt gaacctataa cgaatatact atatgaacaa
     5341 ggagctggag gagctgtaat agaagaccca aaagactttt tattccaaaa gaaaaatgaa
     5401 ctagactggg attatgttga agaagaagta tttaaaaaga atgaagaaga tgatgttctt
     5461 ataaaaactt atgtttctga agaaaaaaat gtgatggaat ttgtcgaaat aataaagcaa
     5521 aaagttttag gattaaaaga ttttggtata gatataggtg aaggaagtgt atcattggac
     5581 caagtaaatg aagctgattg ggcaaatgca tggaaagcat actacaaacc aacaaaagta
     5641 ggacaaaggg tagtagtaaa acctacatgg gaagattatg ctatgcaaga gggggattta
     5701 atcatagagc ttgacccagg aatggcattt ggtacaggga cacatgagac tactagtatg
     5761 tgcattagag aattagaaaa gtatgtgaat aaagattcta aagtatttga tattggctgt
     5821 ggtagtggaa tccttgcaat agcagcagct aaattaggag caaaagaagt tgtagctgta
     5881 gatttagatg aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa taaagttgaa
     5941 aaaagtgtat ctgtaatgca tggtaattta actgatgtaa taaaagataa agcggatgta
     6001 atagttgcaa atataatagc tgatataata aagatacttg caaaagatgt tcaaaacttt
     6061 atgaaagaag atgctatatt catatcttca ggtataattt tggataaagt agaagaagtt
     6121 aaagaaagct taattgaaaa tggatttgaa atagttgaag ttcaaaaatt aggtgaatgg
     6181 tcagcaatag tttctaagct taaaaaataa gggatatggt gacgtataat ggatagattt
     6241 tttgttgaaa aaaataatat aaatctacaa gataaaactt gcactataga aggtgaagat
     6301 gtaaaacata tttcaaaagt tttaagatgc aaattaggtg agaaacttga aatatgtgac
     6361 aagaataata atgagtatat ttgtgagata atgaatatag ataagagtat agttaattta
     6421 gagattttag aaaaagttga tataaataga gaatcagaac ttaaagtaag gctataccag
     6481 ggacttccaa aagctccaaa aatggaaatg atacttcaaa aattaactga agtgggtgta
     6541 gaagaaataa tattagttca aactaagaga agtgttgtta aagttgatga caaaaaagaa
     6601 gataaaaaat ttgaacgttg ggaaagaata atttatgaag cagcaaagca aagcaaaaga
     6661 ggtaagatac caaaattaag aggtgtttta agttttaaag aagctttaga agatatgaaa
     6721 aaaaataatg taaatatttg cccttatgaa aatgaaagaa cagtttcaat aaaacatgca
     6781 ctaaaaaaat gtgattctaa tatagatagt gttggaattt ttataggtcc agaaggtgga
     6841 ttttctgaag aagaaataga acaaatacaa aaaaataatt gtaatgttgt atcattgggg
     6901 cctagaatat taagaactga gacagcttct gttgtagcat caacaatagc tttgtatgaa
     6961 ctaagtgatt taggaggaga aaaataaatt gaaaaaagta gctttttata cacttgggtg
     7021 taaagttaat caatatgaaa cagaagctat gctagaacta tttgagaaag atggttatga
     7081 acaagtcaat agtgaagagt atgctgatgt ttatgttata aatacatgta cagttactca
     7141 tatgagtgat agaaaatctc gtcaatatat aagacgagtt aaaaagaaga atccagatgc
     7201 aataatagcc gttgtaggat gttattctca ggtatctccg gaagaaattt tggatataga
     7261 agaagtaaat ttagtcatgg gtacaaatga tagaagaaag attgttgaag aaattaaaaa
     7321 tataaattca agtaagaaag ttagtacagt tgatgacata atgaaagtta aagcttttga
     7381 agaaatagaa atcagccaaa ctaatggaaa gactagagca tttatgaaaa tacaagatgg
     7441 atgtgacaga ttttgtactt attgtatcat tccttatgcc cgaggacgcg ttagaagtag
     7501 agatattgat agtatagtcg atgaagtgaa aaaattggct aataatggct ataaagaggt
     7561 tgtattaaca ggaatacatg ttgcctctta tggaaaagat ttaaaggata gggatattaa
     7621 acttcttgat gtaataaaac aaataaacca gatagaaaaa atagaaagaa ttaggcttag
     7681 ttctgttgaa ccaattttat ttacagatga atttgtaaat gaagttttaa agatggataa
     7741 agtttgtcct cattaccatt tatcacttca aagtggatgt gatgaaactc taaaaagaat
     7801 gaatagacga tacacaactt tagagtacaa aactattgta gatagattga gaagtaaaat
     7861 gccagacgtg gcaataacta cagatgtaat agtaggtttt cctggagaga caaatgaaga
     7921 gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt tcacaaatgc atatttttaa
     7981 atattcacca agaaaaggaa ctccagctgc aactatggaa aatcaagttg accctcaaat
     8041 gaaacacttt agaagtgaac aacttttaaa tttaagtaaa gtaaatttta ataaatttgc
     8101 aactaaattt atcggccgtg agttggatgt tttatttgaa caaaatatag aaggcaataa
     8161 atatgaggga ttaacttcaa attatataag agttgttgtt gaaagtgaca aaaatataca
     8221 aggtcagata ttaaaagtga aaataaatga tgtaaaagat gaatatgtag aaggaatttt
     8281 gctataacat gtagaaatta taatatttat aagataggag gtggaaagaa tggattgtat
     8341 tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa gtttatgaag atgatagagt
     8401 cttagctttt aatgacttga acccagttgc tccatatcat atattagttg tacctaaaaa
     8461 acattatgat agtttaattg atattcctga taaagagatg gacattgtgt cacatattca
     8521 tgttgttatt aataaaatag ctaaagagaa aggttttgac caaactggat tcagagtcat
     8581 aaataattgt ggaagtgatg gagggcaaga agttaaacat ttgcactatc atattttagc
     8641 tggtaaaaag cttcctaatt acgaagcggg acaaaactaa actaaattat tgaaaaattg
     8701 agttccttct tgataaaaat acttgataaa agtaaaaatt tagtttataa taaacaaagt
     8761 gttaggttgt aatgcatatt atgcaaaaag aacatagtta cagtcccagc ataattgcgc
     8821 tattggaggg agggaaaaag aaatgtcaga agtaagagtt agagagaatg aaacattaga
     8881 cagcgcttta agaagattca agcgtcaatg tgcaatgtct ggcatcatgt cagaagtgag
     8941 aaaaagagag cactatgata agccaagcgt taagcgtaag aaaaaagctg aagcagcaag
     9001 aagaaaaaac gctaagaaat agtagtaaat aagaataaag aggtgaaggg gatgtccctt
     9061 aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa acaaagatac agtgagaaaa
     9121 tctgtagtaa ctttaataag agcttctata aagcagtatg aggttgataa tagggtcgag
     9181 cttgatgaag atggaattat cgatgtaata gcaaaacaat taaagcaacg tagagatgca
     9241 ttagttgaat ttgaaaaagc tggaagagaa gacttaataa aagaaacaga aggtgaaatt
     9301 gaagttttaa aagagtacct acctcaacaa ttaagcgaag aagagttaga agaaattgta
     9361 aaatctacta tatctgaagt aggggcaact tctatgaagg atatgggaaa aataatgtca
     9421 gttatccaac caaaagttaa aggaagagct gacggaaaac ttataaataa gttggtaaaa
     9481 caaaacttac aatagattga tatagcctag agagaaatct ctaggctttt tatagttttt
     9541 gggaataacg agtcatattt gagcatatct ataatcaaag aaaattttga aagaagggga
     9601 aaaatttgat tatatcagta aaagagatta ggaatttttt gataaacata tttataccac
     9661 taataatagg ttacttaagt agcatcttaa caatgattat cagtggaaca gatatttcaa
     9721 cctattattt acaactcaca aagccaagct ttgcaccacc agcttttatc tttcctatag
     9781 tttggactat attgtacata ttgatgggta tatcttcata cctaatatta agaaagggtt
     9841 acaacttacc taaagtaaga gatgcaattt tttattatgg attacaactt gtattaaact
     9901 ttatttggag tatactattt tttggatttg gacttagatt tacagctttg atagatataa
     9961 taataatgat tttggtttct ataataatga tttcgaagtt ttctaaaata gataagagat
    10021 ctggatacat aaatttgatt tatttaatgt ggcttgttta tgcaggattt ttgaactatt
    10081 ttatatggtt tattaataaa tagaatgtaa ag
//
LOCUS       DAFKMX010000146        10055 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFKMX010000146 DAFKMX010000000
VERSION     DAFKMX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.146"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(21..899)
                     /locus_tag="LZB08_002842"
     CDS             complement(21..899)
                     /locus_tag="LZB08_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBM7316330.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKNKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(916..1233)
                     /gene="trxA"
                     /locus_tag="LZB08_002843"
     CDS             complement(916..1233)
                     /gene="trxA"
                     /locus_tag="LZB08_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBM7316331.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1383..2615)
                     /locus_tag="LZB08_002844"
     CDS             complement(1383..2615)
                     /locus_tag="LZB08_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316332.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKIIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2605..3276)
                     /locus_tag="LZB08_002845"
     CDS             complement(2605..3276)
                     /locus_tag="LZB08_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316333.1"
                     /translation="MKVLIVEDNKILLESVVEELNKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3292..4128)
                     /locus_tag="LZB08_002846"
     CDS             complement(3292..4128)
                     /locus_tag="LZB08_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316334.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4154..4828)
                     /locus_tag="LZB08_002847"
     CDS             complement(4154..4828)
                     /locus_tag="LZB08_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBM7316335.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4842..5570)
                     /locus_tag="LZB08_002848"
     CDS             complement(4842..5570)
                     /locus_tag="LZB08_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBM7316336.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5697..6386)
                     /locus_tag="LZB08_002849"
     CDS             complement(5697..6386)
                     /locus_tag="LZB08_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBM7316337.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6394..6957)
                     /locus_tag="LZB08_002850"
     CDS             complement(6394..6957)
                     /locus_tag="LZB08_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBM7316338.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6959..7927)
                     /locus_tag="LZB08_002851"
     CDS             complement(6959..7927)
                     /locus_tag="LZB08_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBM7316339.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENVLKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFISMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVIEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8028..8071)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8436..9065
                     /locus_tag="LZB08_002852"
     CDS             8436..9065
                     /locus_tag="LZB08_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316340.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYKTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9040..9444
                     /locus_tag="LZB08_002853"
     CDS             9040..9444
                     /locus_tag="LZB08_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBM7316341.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9447..9923
                     /locus_tag="LZB08_002854"
     CDS             9447..9923
                     /locus_tag="LZB08_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBM7316342.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEVRLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT         3353 a         1665 c         1013 g         4024 t
ORIGIN      
        1 aaagttaatc aatataaata ttaagccctc tttaatttat ctaaatagta aacagcattt
       61 aaagctgcta tttgcccttg accagctgct tttatataag aatagggttt tccaacacaa
      121 tctccagcag caaagcatcc atctatacta gtcttcatat tattgtcaac tttaatatga
      181 atgccatcaa tctctattcc aggaaccaat gcacttggag ctgtactatc ttttataacg
      241 aatactccat caacttcaat gtggttttct ttaaaagata ctttgtttac aagtttatct
      301 ccatctatct gtactggtct gtcatgtata acttcaatag aatcatctag gttatctgaa
      361 cttctcataa gattatcttt tttgtacata ggtataaaat aagtctttga agtgagttca
      421 cttaaaaaat ttgcttcttc tttagattct tcattatatc ctataactgc taccttttta
      481 tttctataaa gtggtgcatc acatgtagca cagtagccta cacctcttcc tagaaattct
      541 tcttcaccct tgattggtct agtatactct accccagttg caaggattac agtagtagct
      601 tcatacatat catttccaga cattattgta aagtattctc ccatagcata aatattattt
      661 attcttttat tttcaattga aatatccata ctatctatat gtgatttaaa cttgtttttt
      721 aattcatcac cacttatatc ataaaaacct aaataattat ctattgaagg ggctttaaca
      781 agcttattac ttaaattatc attaccaaac atgataattg ttttatttct tatttttgca
      841 tttatagcag cagacaatcc agctggacca ctgccgatta tagctatatc atatctcata
      901 atactcaccc attttttata aatatgattt tacttgcatt tcaattttgt tctttggcat
      961 gaatccaatt aaagtttcta ctggttttcc atctttaaat ataatcatag ttggtactgt
     1021 agatacattg aattgttgag ctatttctaa gctctcatct atatcaactt ttacaaatct
     1081 agctttatcc ttcatctctt cacctaattc tgcaaataca ggtcccaaca tgttacaagg
     1141 tccacaccaa gttgcaaaga aatcaactac aactactccc ttactacctt caacactact
     1201 tctaaattca gatgtattta taacttttgc catttttaaa tcctcctatt aaactttatt
     1261 atccctatat aattatacta tgaaatttat aaatataaat ttacttttat aaggtctttt
     1321 tatattttat actaattagt atacccattc ttcatttcta aaaacaattt tttatatttt
     1381 tattatttta acttttttgg tattgacaat tcaaatgtag tcccaatatc tacatcagaa
     1441 gttacattta tatcaataga taagtttaaa ctaatcatgc gagctattga aagtcctatt
     1501 ccagaacctt ctaaatcttt atttcttaca ttttcacttc taaaaaatct atcaaatata
     1561 aggggtatct cttctttttt tatgcctata ccagtatcac ttataagtaa agttacctca
     1621 tcctctatat cttcttttaa ttctaatgct atctcatcac ccaattttgt atacttaaag
     1681 gcattatcaa tgaaaatcaa tataagctgt cttaatttat ttttatctgt tattataatt
     1741 ttattcttca gtttagaatt aaacacaaat cttttttctt gaaactcagc aatgtcaata
     1801 tagtcttctg atatttcttc caataacttt tctaagtcta tttcttctaa attaactttg
     1861 acaatagagt cctctttagt aagagacaat aaatcagtta tcattttttt aagtcttcta
     1921 gtttccttca ttgcagtagc tattgtttca acttcatcat ttactgtact ttctggggat
     1981 tttaacatac tttctaattt tgatgaaact attgttattg gtgttcttaa ttcatgagaa
     2041 gcatcttgta taaacttagc ttgatttttc catgctgttt ctattggaat taaagctttt
     2101 ctagtgagat atacagccac aaagtaagta attattacag aaattaatat tccaattata
     2161 agaacagatg taagctgtct taaagaattc atctcagaat ctatatttct aattatctgt
     2221 atttggtatc tacctacatc tacatttaat tctctaaaag tatatccatt ttctgtgtat
     2281 ttaaaaaacg aatttttttt atctagtctt ctatctggaa gtaactcatc aaaatattca
     2341 ttttgagtat agtaacttat ccttccatct tcatatacat acaccatatc tttagggtct
     2401 ttgagtctta ttgggttcaa aaatgaagtt cttttaaatt gagagactat atattcaagt
     2461 tcatcattta tctcactatc aattccacta tatgtaagtc ccctaaaata tgagtatata
     2521 aatatagaaa agattattaa aaaactacct actacaataa tatacatttt tattaaatga
     2581 tttttagtac tggtaaatac attcttattt atcatcgaat atatatccta ccttacgttt
     2641 agttttaata tatttatcat atccatattt acttagcttt tttcttagat tacttacata
     2701 tacttccact atttcaatag ttgcatctga atcaattccc catattctat cataaatctg
     2761 ctcttttaat aaaattgaac ctttatttaa tacaaaatac tcaagcaagt taaactgttt
     2821 gttttgaagt tttatttctt ctttttcaat atacactctt ttttctagtg tatctaaata
     2881 taaagattta aattctaatc cgtttctttc ttttatcttt ccattagtcc ttaatatagc
     2941 gtatacccta gctactaact cttccatata aaaaggtttt gtcaaataat cattcgctcc
     3001 tatagtaaat gcttctactt tgtcatctaa tgtttctttt gctgtcaata taagtacagg
     3061 tgtatctata ttattaactc tcattttctt taggatatca aatccattta tattaggaag
     3121 cattaagtct aaaatgacta aatcatatat attttgattt actaagtata aagcttcttc
     3181 tccatcttca cacttttcag tttcaaaatg cttgttaagt tcttctacta cactttctaa
     3241 aagtatctta ttatcttcaa ctattaaaac cttcataagt atcccctacc tttatttgtc
     3301 tatcttaact ttatgagatt ttaagtcaat atccacattg gctttttctt ttatatcatc
     3361 atacgtatac cccactattt caacactttt tgcctttgtt gaacacaata tatctatatc
     3421 tgtctcttct gaaggcttta aaatcttata tgatggtaca cgatttaccg ttaaatattc
     3481 tccattttca ttaacttctg ctgtcttaag aataatattt cctaaatctt ttgatgatga
     3541 attttttact tttacttgat atgtaattcc ctcatttgat tcatttactt ttttaagttc
     3601 tgatgtagac agtacctcat actgactact attttctagc ttaatattat tatttcttat
     3661 attaatagta ttttctttta aattaacata cacaatctta ccctctgcct catattcata
     3721 tgcataaact tctacactct ttacaaaagt tttatgccca cattctatgt aagcaacttt
     3781 cccaggcatt aaagtcatgt ccaacaaagc cttactatct gaagatataa tttttttatt
     3841 ctcatcatat tcattatata tcaattcaac attggatata ttaaatgttg aattattttc
     3901 tacctttgtt gatgtatcta ctttatcacc ttcatctata tttataattt ctgattttcc
     3961 tatattcaat acttttgcat ttccctcatc tatttttatc tcatttttat cttctgtttg
     4021 aatatcaact gaatcaggct gttcatcttt ttcacatgct gtcaacataa aaataaatat
     4081 tgttaatata atcattagag aaattgattt ttttactatt ttattcatat taagcacctc
     4141 atatagtagt attttatagt agatttttat actttttcat aaattctttc aatgcctgtg
     4201 taagtatttg atgtttttca aaattcgaat tactacataa tctttcaaaa tcattccata
     4261 tttctttatc tatacgtatt cttgttgttt ttaattcatc tatgtgttca tcatctatat
     4321 gtacatcttt tttattagat acttttttag gctttatatt gtaatctttt acttctaaat
     4381 accaatcata tacttcacaa agcttatcta aatcttcttg agttatattt atttttctat
     4441 ctttcttagg cttaacattt tgttttggct ttgcattttt taaacctgta tcattaaact
     4501 caatttgttc aacactttta cttaaagcct tttcattaga aatctttttt ttggtgctta
     4561 ctttttttct attacttata gtattctttt tacttttttc atactcctta gctttctctt
     4621 cttccaagtt tctattatcc aaacttttat tataatttaa ttcgtctttt aatatgtatt
     4681 tacctttttc tgacttatat ccttttttat ttaaaaaagt tcttaaagta ctttgactaa
     4741 tctttagatt ttgagctatc tctgcaaatt taaatccttt gctttgaagt tctaaaaatt
     4801 tgtcaattct gtcttcattg ttattcatta atattcctcc gtcaacatct tcttgtcttt
     4861 cttttcatac ttcttattat attcttcttt tctaaatcta ctctatttga ttcaattatt
     4921 ttttgtagca ttttattata agtgaaatca tctaaattat tattttttat atagagaaaa
     4981 gtttcctctt cacatttaat aaaacaaatt gatagcaacc atgccatacc cattttaaca
     5041 taatattctt cactttgtat attattacag tattcaaata tttctttaat atgtttatca
     5101 tcaacataat atattaaaaa cataactaat gcaaatctaa tttcccatgg attctttgaa
     5161 taaacatatt ttttaagtat atcatacata ttttctttat attttttcat agactttaaa
     5221 tcagaacaaa atccatcgca aactgaccaa ttatcgattt taggtataaa tctttttaca
     5281 tagtataaag tttcttcaaa attactattt atattgccaa ttaccatacc ttgtagccta
     5341 acttcttcat agtatgtatc ctctgctact tctaaaaatt ccttccaatt cccttggctt
     5401 atttcttttg ataactttct taaattagga actctaatac ctattatatt ttctgttcca
     5461 ggtataagct ttttattaaa atctctatat tttatatctt gtatagacat catatattct
     5521 aaaagttcat tataatccaa tttattccat tttcccctat caaacatcat ttaattgctc
     5581 cttaattcct tttttcttat tgtaacataa aactctacaa aaaaagcttc taactattag
     5641 aagctttttc aaaaacataa gaaacttttc aattttaaat ttaaactaaa ttaatgctaa
     5701 tctcctacac caaagtctgt attattgtat aaaacttcta tatttggatt ttcatccaag
     5761 aatttatact tactaacaaa ccatttcact aactcttcat ctctttttat actagaagaa
     5821 ttatttataa aaacattcac tgtcgctttt tcaaaagtat ttaggataat ctctatatct
     5881 ttatctatca tctcttttgt ttgtcctttt attccaacca taatacaagg tgaatcaaaa
     5941 tattctttta catcttgtgg agtcttaaaa tttgcatttt tatttaaaat attatttcta
     6001 aaatcattgt caaaggtttc aactccaatt ttaaatacca caggaatttc aaaatactct
     6061 ctcatttcct taagcctatt tttgtaagac caatgacttt ctaaaaataa tttttttata
     6121 ttcttctctt ttataataca ctttattttt tctaaagtat ccttaggtaa ttcaaaacag
     6181 cttccagaat ttatgacttc aagaactcca tatttcccag ttacgttttt taaaaccttt
     6241 agatttaatt tattcatctc ttcttctaaa ttagaattat catctatata atcacaaaaa
     6301 gaacactttc cccatataca aggaaatcct ttaagcagaa caatttctct ttgattttta
     6361 tttgttatct cactatatct atccattttc tatctatttt cctccagtca atgatacctt
     6421 taaagttttt ggaactgcat ttaatcctaa attcactaaa acaactgcta acagcttatc
     6481 tccataatca gtaaatactt gggttacaaa cacactaaaa acatctccca gtcctaacac
     6541 tttcaagatt tgcactatat atgatgaacc tgaagaggtc atacctccaa ataaaaatgc
     6601 tgaaattatt gaacttatta tagatgttgg aatggcaaat aaaagtactc ctagtggagt
     6661 ttttaaaccc cttaaaaaat tctttttaaa tgctattcca gcaagtaatc cggttgatat
     6721 ttgaactggt gcaaaataga gagaatacac atcaaacgta attccactta ttaaacttcc
     6781 aaacacacct gtaacaacag cgtatttagg tcctaaaagt gaagctatca ttatagttcc
     6841 tattgaatct aagtatacag gtaatcttaa tcccatagct ataaaagcgc ctattatatt
     6901 tatagaaata ccaaaagcta taagtgtaat agtttttaca catatttttc ttttcacact
     6961 atatcactcc ttccacagag tctataatgt cttcataacc tttaaaaagc ctctttaaaa
     7021 acatgtacat aaatttcttc tcatcaactt catttagaac aattgcatta ggattttttt
     7081 tatagaaatt gaaagaatcc actatagact gacccatagc tattccatct tctataactt
     7141 ctacatatga ttcaaatcct ttacatatac ttctatctat aaagtacgct actgctagag
     7201 ggtcatttat cacacagcca attattccct cttgttccca atggaaatca atataaaatc
     7261 tagttatttc agttatatac tttgccatct tcttatcaag tctatttata aactcaataa
     7321 tattaggagt aagtacaatt tttctagtta catctaaacc taccatgtgg atttttttag
     7381 ataaattctt gtaaacataa tctgctccat gtgggtctac ccaataatta aactctgcta
     7441 ctggagagca atttccatga atcctaaatg ctcctcccat agatataaac tcatcgagat
     7501 tttcaaatgc tttcttatct ttaataagtg ctttagctat gtttgtaagt ggtgcaagtg
     7561 ctattattga tactttttca tgattatgta aagtatttat tataaaatcc actgctccat
     7621 tttttgcttt agctcccaca accttttgat aaaagttttc tccaatacca tcttctccat
     7681 gtgtatcttg agctgttaca agttttcttt ttagtggtgc ttcttctcct atatatactg
     7741 gaatatttag tgaagaacac atttgaagtg tttttagtac attttctgca cctatatttg
     7801 ctggaacatt tccacaacat gtggtaattc caattacttc tagctctggt gagtttaaag
     7861 ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca atcaattatt acttttctct
     7921 tttccatttt ttatcctcct tgatttgtgg tcgaggagaa ctacacatct tttttccact
     7981 ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc actatataat tccttagttt
     8041 tttatacttg gagttctcga acaagtacag atgtaaaatc atatttttag gctacttact
     8101 atagactctt aaaaactata gactgttaaa aaatttttaa acatctgatt taatagctat
     8161 aaaataaatt ttatagcata aaagaattta ttatatttta atgtatctta ttaaagttta
     8221 tttagaatta tgaaccaact ttaataagat gtggcgcatt taaaccgatt attattatac
     8281 aataatatct taaatattac aattaataac attaatttat tatccataaa aatatattaa
     8341 cttatattat ttaataattt attgacagta ttaaaattaa attatacaat aacagtgtaa
     8401 attaacttaa caccgttaag caaagaggtg attatatgag tatcaaagaa agacgtaaaa
     8461 atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt ataaaccaac
     8521 atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat tctccaacta
     8581 caatttatct ttactacaaa gacaaagcag agattatcag cgatatgacc aataaacttt
     8641 ataaaacagt agaaagtaat gctattgaca ttatgaataa ttgctcatca ctaccaatag
     8701 ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt gaaccagaga
     8761 tggcaaaagc tatcatgcat agtagaatga atattatatt tgcatctgaa aatacaaaca
     8821 atacgcctag caataatggt ataaaaatgc ttgataaatt cctttccatt ggaattgaac
     8881 aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt gcattgttag
     8941 gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt tctcaactta
     9001 tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg caacaaggct
     9061 actaataaaa agaaaaaata tgtaaaaata tctattgctc tattctttgt atttatatgc
     9121 attattattg taattgtaag caacttaaag ccagaaaact tggtagtaaa agatatagat
     9181 attaataatg ttaaaaatgg catttatact ggaagtgctg ataataactt ggtaaaagct
     9241 actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt aaaacacgac
     9301 catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca acagtcttta
     9361 gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa agctgtagaa
     9421 aatgctttaa gaaagggaga ataattatga atactattat tatatattca agtaaatatg
     9481 gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat gtcactattg
     9541 ttgatattaa taataataat aataaaattg agttatcaaa atttgataaa attattattg
     9601 gaagttctat atatgttggt tcagtttcaa aaaaaataca agtgctatgt aatgataatg
     9661 tagagttatt aaacaaaaaa caagttggca ttttcctttg ctgtggcttt tctgaacaag
     9721 cagataaata tttaaaatca aattttccat cctccttatt agaaagtgca aatgctattg
     9781 gaatttttgg tagtgaagtt cgattagaaa aaatgaaatt tttagataaa cttattatga
     9841 aggctgtttc aaaaggtaat tatgacagtt ttagaatatc acaggataat atagataatt
     9901 ttttaataaa tcttaatagc taaaataaaa ctaattattt ctatgataaa ttcttttatt
     9961 tataagtaat attgctcaaa atagtcttta aactttatca aaattttatt tttcttagtg
    10021 aaatctaatg aaactagcac ttaatatata cttta
//
LOCUS       DAFKMX010000147         9901 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFKMX010000147 DAFKMX010000000
VERSION     DAFKMX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.147"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            625..1158
                     /locus_tag="LZB08_002855"
     CDS             625..1158
                     /locus_tag="LZB08_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316343.1"
                     /translation="MDSKVIMVETDRLILRRYIESDLQDLFEYLSNSEVLEFEPYKPM
                     TIEEVKDSLEWRISTDEMIAVELKSTNKMIGNVYLGKRDFNSLEIGFVFNKNYWKKGY
                     AKESCEKLIELAFQSGIHRIYANCDPDNKNSWGLLEKMGFVQEAHLKKNVFFWRDNNN
                     QPIWKDTFIYSKLNSPK"
     gene            1389..2297
                     /gene="trxB"
                     /locus_tag="LZB08_002856"
     CDS             1389..2297
                     /gene="trxB"
                     /locus_tag="LZB08_002856"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBM7316344.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(2387..4210)
                     /gene="typA"
                     /locus_tag="LZB08_002857"
     CDS             complement(2387..4210)
                     /gene="typA"
                     /locus_tag="LZB08_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBM7316345.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            4422..4664
                     /locus_tag="LZB08_002858"
     CDS             4422..4664
                     /locus_tag="LZB08_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316346.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4812..5003
                     /locus_tag="LZB08_002859"
     CDS             4812..5003
                     /locus_tag="LZB08_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBM7316347.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            5294..7795
                     /locus_tag="LZB08_002860"
     CDS             5294..7795
                     /locus_tag="LZB08_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBM7316348.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7955..8623
                     /locus_tag="LZB08_002861"
     CDS             7955..8623
                     /locus_tag="LZB08_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316349.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            8607..9884
                     /locus_tag="LZB08_002862"
     CDS             8607..9884
                     /locus_tag="LZB08_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBM7316350.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         3893 a         1118 c         1709 g         3181 t
ORIGIN      
        1 actacactat attacaaaaa aagtttacaa aactttaatt tgaaaaatgt aattatatgg
       61 tatatcatat aaatataagt aaatagtgct aatatttaaa ctattaatca taattggttc
      121 aacaaaagta tatagttaat aatgtttaat ttgaagaaat tgttagtgtg aaattacata
      181 tatattcaaa ttaaattcat aaatatttca cataataata gtatactgta aacaaaagga
      241 ggtattaaat atgggaatag tacttataac agcagcatta gttttggtta tgtatggact
      301 ttcaattcta tcatatatat cagaaagcaa tatattagaa gacactgtaa atgataacca
      361 ataaggtttt gaattatata aataagtgag aactgcaaat aaaagtttat ttttatttgc
      421 agttttttta tatatatgta aaataaagtt aattaataaa aaataatgct tatattgaaa
      481 taaaggttta ttaaaatttt tatgatgata tgttcacatt tacctgatag acatttagat
      541 attaggttta agaaaatata gtgtgttgta catataagct gaataatatt tagtaaaaaa
      601 attaaagttt gaaggggaag tgtaatggat agtaaagtga taatggtaga aactgataga
      661 ttaattttaa gaagatatat agaaagtgat ttacaggatt tgtttgaata cttgtcaaat
      721 tctgaagttc tagagtttga accatataaa cctatgacaa ttgaagaagt aaaagatagt
      781 ttggagtgga gaatttcaac tgatgaaatg atagcagttg aactgaaatc tactaataaa
      841 atgattggta atgtatatct tggaaaaagg gattttaatt cgttggaaat tggattcgtt
      901 tttaataaaa actattggaa aaagggatat gcaaaggaaa gctgtgaaaa gttaattgaa
      961 ttagcatttc agagcggaat ccatagaatt tatgctaatt gtgacccaga taataaaaat
     1021 tcttgggggt tattggaaaa aatgggtttt gttcaagaag ctcatctgaa gaagaatgta
     1081 tttttctgga gagataataa taatcagccg atttggaaag atacattcat ttattcaaaa
     1141 ttgaatagcc caaaatagat gaacttaagt ttggtaagtt tatttatgtc agtttaaagt
     1201 atataatttc atagtttaag aatgaaaaat agcattttac agtgtagtaa ttaactttta
     1261 aattaatact ttttgtacac taataaaaaa taatgttttg tttatagtat atatggataa
     1321 taatagtaaa gtataagaat gatgagaata atatataaga aataataata taaagagagg
     1381 taaacgttat ggtagatatc attgtaattg gtgctggacc agcaggttta acttcagcaa
     1441 tttatgctat gagagcaggg ttaagcgtta cagtatttga aaaaaatatt tatggaggtc
     1501 aagttgcaag tacctctgag gtagaaaatt atccagcagt tcaaaagatt tctggggtag
     1561 aattttctaa taatatatac aatcaagcag ttgctcaagg tgttgatatt cagtttgatg
     1621 aggttgaaga gataaattta gagggaaaag taaaggttgt aaaaacttct tcaggtgaac
     1681 ataaagcaaa ggctgttatt cttgcaaatg gagttgaaag acgtaaatta ggttgtgcag
     1741 gggaacaaga atttacaggt agaggagtat cttactgtgc aacatgtgat ggagcctttt
     1801 ttaaagataa agaagttgct atagtaggtg gaggaaatac tgcactagaa gatgctttat
     1861 ttttagctaa taattgtaca aaagtgtatt taattcacag aagagatagt tttagaggtg
     1921 aagaagtgtt ggagaaatct gtgaaagcaa gagagaatat tgagatactt tacagtcatg
     1981 gtgtagaaaa aatagaagga gaaaagactg tatcgaaaat agaagtaaaa aacttaaaga
     2041 ctgaagaaaa aagaactata gatgttagtg gtatttttat tgctataggt cttaaaccta
     2101 ataataaaat gtttgaaaat gttcttgatt tggatgaagg tggatatata atttcagatg
     2161 agtcttgtac aacatcagta gaaggtgtat atgtagcagg agatagtaga actaagtttt
     2221 tacgtcaaat aataacagca gcatcagatg gagctattgc agccgttcaa gcagctaatt
     2281 atataaatgt tgaataatat aaatttcaaa taaaaatagc catataaact gaaataattt
     2341 ttcagtacta tatggctatt ttgttaatta gaggtaaatg tacgttttat ttaccttgtc
     2401 tagatttgtt atatcttaat ctatcatgtt catttaagaa tctttttcta agtcttatag
     2461 aatcaggtgt aatttcaact aattcatcat cttctataaa ctctagtgct tcttcaagag
     2521 taaatattct aggaggagat aattttatag catcatctga accagaagca cgtacgttac
     2581 tcattttctt gtttttacaa gggttgacta ccatatcatc ttttctggaa ttcataccaa
     2641 ttatcatacc ttcgtaaact tctacaccag ggtctacaaa cattacagct ctatcactta
     2701 aggcattaag agaatacccc atagtaacac caggtccttg tgctattaaa acaccatttc
     2761 ctcttgaagg tatttctcct ttaaattctt caaatttttc aaatgaacga actaaagtac
     2821 cttctccacg agtagcgttt ataaattcac ttcgatatcc taaaagacca cgagtaggtg
     2881 ctaagaactc tatttttaca tagtctcctt ctattgacat agactccatc ataccttttc
     2941 tcatgtttaa ttcatttata atagtaccag agtaaacttc aggacagttt acaacaactc
     3001 tttctattgg ttccattaat tttccatctt ctttatgcat taaaacttca ggtttagaaa
     3061 caccaacttc ataaccttca cgtctcatat tttcaagtag tatagataag tgaagctctc
     3121 cacgtccaga aactttgtat ccatcagtag tatcaagagg ttcaacttta agaccaacat
     3181 ttacttcaag ttccttttca agtctatctt tcaaatgtct agttgtaacg aattttccac
     3241 tttttccaac gaatggagag tcgtttacta agaagttcat agaaagagta ggctcttcta
     3301 tttttatcat ttccataggt agtggactat ctaaatcgca tatagtttca cctatagata
     3361 tatcaggaat accagctata acaactattt ctccactagt agcttcatca acttcaactt
     3421 gttttaaacc ttcatacaca gaaagttttg atacttttcc tttagataca acagaatctt
     3481 ctctacatat agcaacttgt gtattgtttt taagagtacc tttgtaaatt ctacctatac
     3541 caagtctacc tacataatca tcatatgcaa gagctgatat ttggaattgt agaggttcat
     3601 tatcataatt aggataagct tcaacgtgat ttacaatagt tttaaataga ggagaaaggt
     3661 cttcactatc atcatccatt tcaagtttag caataccttg tttagcaata ccatatatta
     3721 taggaaattc gcattgctca tctgttgcat ttaagtctac aaataaatcg aaaacttcat
     3781 taactacttc ttccgctctc tggtcttttt tatcaatctt gtttataaat aatattggtt
     3841 ttaaaccgaa ctctaaagat ttttgtaaaa cgaatctagt ttgaggcatt gggccttcac
     3901 tagcatcaac taatagaata actgtgtcaa cagttttcat aacacgttca acttcagaag
     3961 aaaagtcact gtgtcctgga gtatcaacta tgtttatttt gtaatcttca tagtttattg
     4021 cacagttttt tgaatatata gttatgcctc tttctttttc aaggtcgtta ctgtccataa
     4081 cgcagtcttt aactacttcg ttctttctaa aaacaccgct ttgagaaagg aaagcatcaa
     4141 ccaatgttga ttttccagcg tcaacgtggg ctataactgc gatatttata attttatgtt
     4201 tttgtgacat ttttgttgct cccttcgatt aaaataactt attaattata gcacaatata
     4261 ttattaggta caaggaggaa tatattaaat tgtattaata tagtttaaat tggataaaac
     4321 tgttagtttt gcatctgaaa attattctgt ttaaaaaaat tttttagaca aataataacc
     4381 ttaatagata aaaagattaa taaaagagta aaggatgata tatgttggaa gtaaccaaaa
     4441 taataattaa atattttgca agtgaaagtg atacttttgt aaaagaagaa gtttgtaatt
     4501 ttgaagaact tcttaagtgg tctcataaat ttattgaagg taaaaagata gaagatgttt
     4561 tgtatataaa gtctaaagaa aactataaag taaatgtgat taagtcttta gaaattaagg
     4621 aattggaaat agtgagaaaa caacagatac caccgtacat gtagtattaa taatatataa
     4681 aattaatatg taatactcgc aataggatat aaatggtata tatggtaata atttatatta
     4741 aaaaaacttt tataaagttt atatttgggt ataaaagtat taattagaca ttaattagga
     4801 ggaacttaaa tatgataaga gtatgtccaa actgttcaaa tgtggatgta aataagctaa
     4861 aaactttggt tggagaagaa aatgtaaaga ctggttgtat aggtcagtgt agagcattta
     4921 aaaaagaagc tgttggaata atagacaatg agcttgaaat aaaggaaaca gaagcagagt
     4981 tttttgaagc atgtaaagaa taaattttaa ataaaatata aattataata aatagagcat
     5041 ctaaattgta taagcattaa acaatttaga tgctctattt ttatatatac atttaattat
     5101 gagtatactt ttctattaaa tacaaataaa tataactaat acatttattt attaaaaaaa
     5161 tattgattta atatacctat agggggtata ataaaaatat agatttctaa ataagtaatt
     5221 tatcaatatt acatatcaaa ataaaacgtt acaaattagc tgaaaatata ttgaaagaaa
     5281 ggaagatttt attatgagtt caaataaagt ggtagaaagt tataaaatta caggtatgac
     5341 atgtgcagct tgtgcaaagg cagtagagag agtaactaag aagatggatg gagtatatga
     5401 ccaaagtgta aatatagcta ctgaaaaact taaaatagaa tatgataatt ctaaggttaa
     5461 ttttgatgat ataaagcagg ttgtagaaaa agcaggatat ggaattataa aggaagaaag
     5521 taataaaaaa attgatatga aaattgatgg aatgacatgt gcagcttgtg caaaagctgt
     5581 agagagagtg gtaaaaaaat tggatggagt agaaagtatt agtgtgaata ttgcaacaga
     5641 taaagctaat atagactatg acccatcaaa agtaaaatta tctcaaataa aagcagctat
     5701 tgaaaaagct ggatataaac caatagaaga ggtaagaaat aaggttgatg tggatgaaga
     5761 taaattgaga aaagaaagag aaatgaagtc tttatttgtt aaatttatag ttgcaatagt
     5821 atttgcagta cctctgtttt atatagcaat gggacctatg ataataaagc ctatagggcc
     5881 atggcctttg ccagaaatta taaatcctat gacaaatact tttaattatg cattaattca
     5941 gttaatttta gtaataccag taatgatagc tggttataaa ttttatataa atgggtttaa
     6001 atcacttttt tctttaagcc ctaatatgga ttctttagtt gcaataggaa cattagccgc
     6061 atttttatat agtctatata cgacactaca aatagccaat ggacaaattc aaggtatgca
     6121 ccatcatcag ttgtattatg aaagtgcagg aataataata gcacttatct tacttggtaa
     6181 atatttagaa tcaaaatcaa aaggaaaaac atcagaagca attaaaaagc ttatggggct
     6241 acaacctaaa actgcaatag tgttagttga tggtaaagaa gttgaaactc ctatagaaga
     6301 agtagagata ggagatatac tattagtaaa accaggaact aaaatacccg tggatggagt
     6361 tgtaatagaa ggatatacat cagttgatga atcaatgctt acaggtgaaa gtataccagt
     6421 agaaaagaat gttggaagta aggtgacagg agctagtatt aataaaaatg gtgttattaa
     6481 gtttaaggct gaaaaaattg gtggagatac tgctcttgcc caaataataa agttagttga
     6541 agatgcacaa ggtacaaaag cacctattgc taaacttgcc gatactgtat ctggatattt
     6601 tgtaccaata gtaattgcaa tagctgtagt tgcatcatta ttgtggtttt taataggagg
     6661 aaaagatata gtatttgtac ttactatatt tatatcagta cttgtaattg cttgtccttg
     6721 tgcattagga ttagcaacac caactgctat aatggttggg actggaaagg gcgcagaaaa
     6781 tggaatcttg ataaaaggtg gagaggcact agaatctgca cataaagtaa atacagtgat
     6841 atttgataaa acaggtacta taacagaagg aaagccaaaa gtaacagata tagtattaaa
     6901 taataatgta aaagaagaat atcttatcaa gattgcttca agtgcagaaa aaggttcaga
     6961 gcatcctctt ggagaagcaa tagttaagta tggagaagag aagaatataa aatttgaaaa
     7021 agttgataat tttaaagcca taccaggtgc tggtatacaa gttactatta atgatgaaag
     7081 tatattactt ggaaatagaa agcttatgaa tgacaataat ataaagcttg gggatttaga
     7141 agaaaaatca aatatattgg caagccaagg taaaacacct atgtatatag ctgtagatgg
     7201 aaatttatca ggtataatag cagttgcaga tgtagtcaaa gaaagtagta aaaaagcaat
     7261 agaaatactt catgatatgg gaataaaagt tgcaatggta actggagata atgcgaaaac
     7321 tgcaaatgct atagcaaatc aagtaggtat agatatggtt ttggcagagg ttttaccaga
     7381 agataagtca aaagaagttg aaaaactaca aaatcaagga aagtttgttg caatggtagg
     7441 ggatggaata aatgatgccc cagctcttgc aaaagcagat atagggatag caataggaag
     7501 tggaacggat gtagccattg aatctgcgga tatagtactt atgaagagtg atttaattga
     7561 tgttccaaca gcaattaaac ttagtcatga aactataaaa aatataaaac aaaacctatt
     7621 ttgggcattt ggatataata caattggtat acctgtagca gcaggaatat tatatgtatt
     7681 tggaggacct ttattaaacc caatgattgc agcagcagca atgagcttaa gttcagtttc
     7741 agtcgtatca aatgcactta gacttaaaaa cttcaaggca tataagagag attagattaa
     7801 taaattaaat aaaatgtatc caaaagagct atctttaaat taaaaggatt aatttacaaa
     7861 ttaagatagc tcttttttca tgtaaaattc acaacaataa gataaaatat aagtaatata
     7921 cgaaatttta aataaaatag aaaggattga tagaatgaat tcgtcaatac ttgtaattga
     7981 agatgattca aatatacaag agttaatatc agaattttta agtgcagaag gttatcaagt
     8041 tgatacagca aatgatgggt tggaaggtat acaaaagttt aagcaaggaa gttatgattt
     8101 agtcatatta gatataatga tgccaaactt agatggttat ggagtttgta aaatgataag
     8161 aaagtcatcg agcgttccaa ttatattttt aactgcttta aatgatgaag gagaccaact
     8221 taaagggttt gacttagaat gtgatgatta tataacaaaa ccattttcat ttaatcttct
     8281 tataaaaaga gtagaagcaa ttttaaggcg aagtaacaag actataaatg acaaatttat
     8341 agtttttgaa aaattaaaat tagatttaaa cacatatatt gcagagatag atggagaacc
     8401 tatagaactt acactaaaag agtttaatat attaaaagct ttgatagaaa agtatccaca
     8461 agttataact agggaaggtc ttttggatag tatatggggt tatgactatt atggagatac
     8521 taggattgtt gatgcacata taaaaaatat aagaaaaaaa atatcattac catatattaa
     8581 aactgtaaaa ggaataggat atacacttga gaaggatatt tgataagtgg gaaaagttaa
     8641 gtataaagta taagttattt tcaataacga catcattatt aatagcatta gctttaataa
     8701 tatatctaat tttatatttt ttattgccat catattatca tgagtataaa attgagtctc
     8761 ttcaagaaag tttgaaatct ttagtagata gttcaattca tttcgataca tatactttag
     8821 aagaaagact ttattatatg gctaaagacc aaaatttggc tattttatta aaagataacc
     8881 aaggtaaaat tgtatatggt aagaatgaag tagttatttt aagatatagt aagtacatga
     8941 ttaatagtct tgaggatgag tatagaactt ctatacctat atatactaaa gatgcaaaag
     9001 atggtccata tactttagaa ctcgttatgc cattacaacc aattgacgaa gcaaacgaag
     9061 ttataagaaa actaatgcca tacatcatat caatagcaat attgatagcg attattggag
     9121 catatatata ttcaattgta ataacaaagc ctcttataaa cataatagag agtgagagag
     9181 aacaggaata tagaaggaag gattttgttg ctacaatatc acatgaacta aaaactccaa
     9241 taaccataat aagtggtcaa attgaaggta tgatttatag tgtaggtaag tataaggata
     9301 gggatactta tctgaaaaaa tcgtatgagt gtacacaaga attaaaagat ttagtaaatg
     9361 aaatgataga ggtttcaaaa tcagaaatac ttgaaaaaga tttgaaattg gtatcaataa
     9421 atataagtga attattaaat agacttgtaa agagacaagt atttttaata gaagaaaaac
     9481 atatgaaaac tattttaaaa atagaagaaa atttggaaat taaggcagac caagagagaa
     9541 taactaaggc aatcaataat ataataaata atgccataaa atattctcct gaagaatcag
     9601 agcttataat tagactctat gataaaaata aacgaataag taaaaagact tctaatcaaa
     9661 gagtggtttt agaaattgaa aatactggtg taactatcga gaaacgttat ttagaagaaa
     9721 tatttaatcc attttataga atagaaaaat ctcgttcgag aaaaactgga ggaagtggac
     9781 ttggacttta tatagtcagc caaattttta aaagtcatgg gtttgattat agcataaaaa
     9841 acaaagaaaa ctctgtagta ttcacagtag aatttaaaaa ttaattactt attataaaga
     9901 a
//
LOCUS       DAFKMX010000148         9896 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFKMX010000148 DAFKMX010000000
VERSION     DAFKMX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.148"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            99..890
                     /locus_tag="LZB08_002863"
     CDS             99..890
                     /locus_tag="LZB08_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBM7316351.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            919..2139
                     /locus_tag="LZB08_002864"
     CDS             919..2139
                     /locus_tag="LZB08_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBM7316352.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIISFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2159..2854
                     /gene="pxpB"
                     /locus_tag="LZB08_002865"
     CDS             2159..2854
                     /gene="pxpB"
                     /locus_tag="LZB08_002865"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBM7316353.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENRDEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2884..3870
                     /locus_tag="LZB08_002866"
     CDS             2884..3870
                     /locus_tag="LZB08_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBM7316354.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4089..4655)
                     /locus_tag="LZB08_002867"
     CDS             complement(4089..4655)
                     /locus_tag="LZB08_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316355.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5007..6074
                     /locus_tag="LZB08_002868"
     CDS             5007..6074
                     /locus_tag="LZB08_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBM7316356.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6093..7253
                     /locus_tag="LZB08_002869"
     CDS             6093..7253
                     /locus_tag="LZB08_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBM7316357.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7266..9362
                     /locus_tag="LZB08_002870"
     CDS             7266..9362
                     /locus_tag="LZB08_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBM7316358.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9521..9865)
                     /locus_tag="LZB08_002871"
     CDS             complement(9521..9865)
                     /locus_tag="LZB08_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBM7316359.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
BASE COUNT         3751 a         1141 c         1831 g         3173 t
ORIGIN      
        1 acaagtgata aaatatttca tgcaatatat aaaataaaca aaatttaaaa attaaaacta
       61 cttattctat caaaatgtta gcgaggaggg gtcataaatt gtataaagta gatttaaata
      121 gtgatttagg agaaagtttt ggaacgtata agattggttt agatgaagaa gttttaaaat
      181 atatttcatc agctaatata gcttgtgggt ttcatgcagg tgaccctagt catatggaaa
      241 aaacagtaca gctagctaaa aaaaatggag tgaaaatagg cgcacatcca ggtttcctag
      301 atttaatagg atttggaaga cgtgagatga aaataacaaa gcaggaagct aaagattata
      361 caaaatatca attgggtgct ttaatggcat ttgcaagttc aaatggatgt aatatacaac
      421 atgtaaaacc acatggagca ttatacaata tggcagctaa agataaagag ttagctatgg
      481 ggatttgtga agctatatat gaagtcgata aagatattat acttttgggg ctatataata
      541 gtgaaatgat aaattctgct aaagaaattg gacttaggtt tgcaaatgaa gtatttgcag
      601 atagagctta tgataataat ggttttttgg ttccaagaaa tgtagaagga gcagttatac
      661 atgatacaaa acatgcaata gatagggttg ttaggatggt aaaagaagga actgtagaaa
      721 ctttaacagg tgaagttata catatcaaag cagattctat atgtgtacat ggggacaatc
      781 caaaagcaat agagtttgtc aaagaaataa gaaagcgttt tgaattagaa ggtatagaag
      841 tttgtccatt agaaaacata gaagtttgtt cgtcagagaa catagtataa gactgataac
      901 atgaggggga gataaaatat gagtacaaaa aatattacag ataaaactaa aaataaaaaa
      961 gatgtcggag cacttatagg ggctgcattt ataatggcaa catcagcaat tggaccaggg
     1021 tttttaactc agactgcaca atttacccaa gattttggac cgaatttttc atttgtaatt
     1081 ttaattacaa caatcctttt tataggggct caagtaaatg tatggagagt aattggtgta
     1141 tcaggactta gaggtcagga tatagcaaat aaaataatac caggattagg ttatttagta
     1201 gcttttttag tagctcttgg aggattagct tttaatatag gaaatgttgg tggagctgca
     1261 cttggtatga atgtaatgtt taatatgaat atgacattag gaactgtatt aagtggatta
     1321 atagctatat ttgtatttat gtcaaaaaat tcaaattctc tagtcgataa gataactaaa
     1381 tttttagcat taggaatgat aattatagtt ggatatgtag caatatcaaa tcatcctcct
     1441 gttggagagg cagtatcaag aatggtaaaa ccagaaaatc caaaaggact gatattccca
     1501 ataataactc tacttggagg aagtgttgga ggttatataa cttttgctgg aggacataga
     1561 ttaatagacg gtggaataac aggagaagaa aatataaaag aaataactaa atcttctctt
     1621 ttgggtatat tagttgccac aatgatgaga gttcttttat ttttagcaat actggcagtg
     1681 gtttcaaaag gtcttcaact tgacccagaa aatccagctg cttcagcttt taaatttagt
     1741 gcaggagcaa taggatataa attctttgga cttgtattat ggtctgctgc aattacatca
     1801 gttataggag ctgcttatac atcagtttct ttcttaaaga cactaaatcc ttttatagat
     1861 aaatatgaaa aatattttat aatatcattt atagcaatat caacacttat aatggcattt
     1921 ataggaaaac cagctacttt attaatttta gcaggagctt taaatggatt gatacttcca
     1981 atcacattag gaattatgct tatagcatct aaaagaaaag atattgttgg agattacaag
     2041 catcctacat ggcttttaat atttggatta atagtagttt taatatctgc atatacagga
     2101 ataacttctc ttagcagcct aggtgcttta tttgcttaat ttagaagagg tgacatttat
     2161 ggaaactaga tacttgatat caggggataa ggcagtggtt gctgaatttg gaaatgagat
     2221 atcagaagat ataaacaaaa aagtaatttc atttatgaga gcaatagaaa tatcgaatct
     2281 taaaggtatt gtaacagaaa tggtaccaac atataggtca cttatgataa gttataatcc
     2341 attagaaata gattttgata gtttgattga gaatttaaaa aaaatagaag ataatttgga
     2401 aagtatagta ttaccaaaac ctaaaataca tgagattcca gtatgttatg atgaggtttt
     2461 tggtatagat attaaaaacg ttgcaagcta caataatctc actgtagatg aagttataaa
     2521 aatacacaca agcagagagt acttgatata tatgctaggg tttactcctg gatttccata
     2581 tctaggtggt atggatgaaa gaatagctac acctagatta gaagtgccaa ggactaaaat
     2641 ttatggtggg agtgttggaa tagcaggttc ccagactggt gtatatccta ttgatagtcc
     2701 tggtggatgg caaataattg gtaggacacc tttaaaatta tatgatgaaa atagagacga
     2761 acagattttg cttagagctg gagattttat aaaatttgtt ccaataacat tagatgaatt
     2821 tatagaaatc gaaaaaaata acttaagtat ttaaatatcg ttatagtaca gggagtggta
     2881 tatatgggat tcaaagttga attaggaggt ttccaaacat taatacaaga cagaggtaga
     2941 gttggctatg gtcaatatgg tgtatctgga tgtggagcaa tggatgagta tgcacataga
     3001 gtaggaaata tattagtagg aaattctgaa gatgaggctt ctttagaagt tttaatgtta
     3061 ggtcctacaa ttacctttga tgaatatacg caaatagcag taactggggg agatttaggg
     3121 gctaaaataa atggaaaaga aattcaaaac tggaggtcat atcaaataaa tccaggtgat
     3181 gtattatcat ttagaggtgt gaaatcaggt gctagagcct atgtgtcaat agctggtgga
     3241 atagatgttc cacttgcaat gggaagtaag tcaacttata caagagctaa aattggtgga
     3301 tttgaaggta gagcacttaa aaaaggagat tatataaata cttttattca ggaaaaagat
     3361 tttacaataa ataaaaaatt aagttcaaaa tatattccaa cttatagttc agaaatcgta
     3421 ctaagaattg taaaaggtcc tcaatttgat gctttttcta acggtgaagt agagaaattt
     3481 ttatcaaata aatacaaagt tacaaatgaa atagatagaa tgggttgtag actagatgga
     3541 gagagtataa agcaccttaa tggagcagat attatatctg atggtatttc ttatggagct
     3601 atacaagtac ctggtcatgg aaaaccgatt ataatgttat cagatagaca gactagtggt
     3661 ggctatacta agatagggaa cgttataagt gtagatttgt ataagttagc acaagcaaag
     3721 ccaaatgatg tagtaaaatt tgaacttgta gatatatatg aagcacatag actattgaga
     3781 gagcaagaag ataagattca agatatctac aagagtatga aaaatataag agttgtaaag
     3841 gcaaaagtat taaatgatat agcagtttaa tttaatttta acaaatacaa tttttattat
     3901 aatttataat ataacttaca aatagctatt tactatttac cataattaag atggtttaga
     3961 ttagtcaaat agctattttt ttagtttgag taaatatttt aaatttaaat aaatacttca
     4021 attttaaaat aaaagcaata tttaaatcta ttatattatc tacaaaaagt aggtgtgtta
     4081 tattgagttt atttagattt tattggccaa attggaggtg atataactcc atatccacga
     4141 gctattgtat ctttacttgg attgatgatt ttatgtggta cattagcttt tataaatgtt
     4201 gagtcacctg gatttaatct aactaccttt tcacccatac aaatatcaac cacaccactc
     4261 gttaccacca taatttcttc tgatgcatga tatatataat ctttggcatc ctcactttct
     4321 ggtttaagtt caaactctac aaataaaatt gaaggtgtaa actcagttgt aggcattgga
     4381 gatactatct cgtaaaacat ttcactcttt ggaaatttca tcataactct atcttctttt
     4441 ctaatgacta aatcatcagt attattgtca tccataaaca tatataaagg agtgtctaaa
     4501 acttcagcga tttttctcaa tgaagacaag gatggttcaa tagcatctct ttctatttga
     4561 gagatataac ttatagataa atctgtcaat ttggaaacat cacttaatgt ttttttcttg
     4621 ctttttctta tatttctcat cttttcacct agcataagaa cctccaaata ataaaattaa
     4681 tataaaatat tcgcttaaaa caaagtaatt atagcacaac atagaagtag tggcaataag
     4741 aatacatagt agattttgac aattaaaatt taattaaatc caaataaaag tctatgaaaa
     4801 tgaattattg ttaaattaat ataaagaaaa taataataaa aaaatttaat agaagagtat
     4861 taattgaatt aagattataa cattatattt tggaaaagag taaattttca gtaaattatc
     4921 ttgacatata tattgaagta ataatataat ttaaagtata aaattttaaa aattgaatta
     4981 taacttcaaa aaataaggag ggtaatatga agataacaga tatcaaattt gaaaagctta
     5041 gaattaaatt aaaaaaacct gtagtagttt catttggagt aattgaatat ggagaaagtg
     5101 taattttaaa aattgaaact gatgaagggt attgtggatt tggcgaagca gcacctcttg
     5161 cagcagttac aggtgaagtt ttggataatg ttctttcagt attacttatg tttaaaaaag
     5221 agttaatagg caaagaccca ctagatattg aaaccataca taccatcatg gatggaatta
     5281 ttataggtaa tacttctgca aaagcagcta ttgacatagc tttatatgat attaaaggaa
     5341 aaattatgaa tgcacccctt tataaagttc ttggaggatt tgatagtaaa gtccaaacgg
     5401 atattactat aagtatagat aagccagaaa aaatggcagt agaagcttta gaaagagtaa
     5461 aagaaggttt taggatattg aagcttaaag caggtataaa tcctgaagac gatattgaag
     5521 ctgtaaagtt aattagagaa gccgttggag agagtataag aattagaatt gatgcaaatc
     5581 aaggatggaa cgtaaatagc agtataaata ctatcaaaaa gttagaagaa tttaatgttg
     5641 atgcaattga acaagcactt ccacattggg atttagatgg tactgcttac ataagaaata
     5701 aaagtaacac aaaaattatg atagatgaat cattacattc accaatagat gcaattaagg
     5761 ctataaaaaa gaatgcagtt gatacattta atattaagct gatgaaatca agtggaatat
     5821 atcctgctat aaaaatcaac aacatagctg aggcaagtgg agtaaattgt atgttaggat
     5881 gtatgctgga aactagaatt ggtataacag cagcagcaaa tttaatagca tctaaaaaga
     5941 acataacaga agctgacctt gatagtttta tgttttgtga agaattagaa ggtatatctg
     6001 gaggatttgt aatggatgga gatattatga atcttgtcaa taaaccaggt ttaggaatag
     6061 aagtaaactt atgataaatt tggaggttaa atatgaatac acttttttca aatccaggta
     6121 gtatacttgc tgtaatgaca tcaatgattg cattgggttt ttacttacaa agatacaaag
     6181 ccattaaaag cttaggacca gcacttacaa taatcataat gggaataata ctttcaaact
     6241 taaaagtagt tccagtaagt actgagttat atggaactat ttcaacatat gctattccag
     6301 tttctatgac tataatgttg atgagtgttg acttaaaaga aatgacaaaa ttatcaagag
     6361 aacctttaat tgcaattttt gtagctgtat taactgtaag tatcatggca tttttatttg
     6421 gattagtatt tgcagaaaaa atatcagaag gttggaaggt tgctggaatg tttgtaggta
     6481 catatactgg tggaagtgct aatcttacag ctattggaac aggtcttaat gtaagtagac
     6541 aaactcttgc tgcagcaaat gcagcagact atgtaattgg agtaccaaca ttgatattta
     6601 tgtttgctct accagcaata ttaaaaaatt caaagaagtt taaaaaactt tggccatatc
     6661 atgtagaaga atcagaatta gaggattgtc aaaatgaaga atttatggag tcaaaagaat
     6721 ggagtattaa agatatagct tggatgttgg ctataggttt tgttgttaca gaagtttcaa
     6781 caatacttgc tggatatttc aattcaagtt ttagtagtgc agcaagaata cttttagtta
     6841 caacaatttc aattataatt gctcaattaa agccagtaaa gaaactaaag ggaaatttag
     6901 atttaggatt atttgttgca ttattctttt tatgtacaat aggtttttca gtagatataa
     6961 aagagttttt aggttcaact tttactataa cactttactg ttttagcata atttttgctt
     7021 catttgtttt ccatttaggc ataactagac ttttaaagat aaagtatcaa tatgtaatac
     7081 tttctatagt aggagcaatt gctgatggtc ctacttcagc tttagtagct gcttctgcaa
     7141 agtggaattc gcttgtaagt gtagcagtcg taatgggagt aattggtgga gtattaggaa
     7201 attatgcagg tatatcagtg gcatatgcaa taaagatgtc tctaggatta taggggggag
     7261 tttaattgat aaaatttaaa ttatacatat ctggactata cagtggaaat gtaatctttg
     7321 atggagattt attaattgaa aagttgaatc cttttacaaa taaaatagaa agtttaaaac
     7381 caataagcaa agaagaaaat acatactatt taaatcttac aaaaatagat ttaaagtctt
     7441 tatttaataa ctttgatgta tatactaaaa gcttagtaaa cacagataaa gacacagtta
     7501 taaataactt aggagaaacg cattctaaac ttaatgaata tatatgggtt caaagaaaca
     7561 aaaagttccc actagatata attattgtag ataataaaat agtaggtttt atctgtttat
     7621 ccagagaaac ttgtactata ttaattatgg atggatatga agagtatact gtattaaaag
     7681 aatgggaaaa aactcataaa aatgaagaga tatattctat aagatttggt ggaaattata
     7741 tgattgatat gaaagatggt ataaaacttt ctacagatgt atatctccct gattttgtag
     7801 attctaccaa aaaagcacca acaattctta tgagaactcc atatggaaaa gaaaatgata
     7861 aagaaatata ttataaatat gtacaaagag gatatgctgt tgtaatacaa gatgttaggg
     7921 gaagaaatga atcagaaggt aagtgggaac ctatgattca tgagagggaa gatggagata
     7981 gtaccataaa ttggatagta tcacaggaat ggtcaagtgg aatagttgga atgcttggag
     8041 catcatatct tggttatgtc caatgggcag ctgcatcttc aggcaataag catcttaaag
     8101 ctttagttag tatagtaaca tcaggaagcc cttttattga tataccaaga aaaggaggag
     8161 cttttgtatc tggtatgctt gcatgggcat ttatggtatc aagaaataaa gttgatagaa
     8221 gcaaaatggt aagagatgat tgggatgatg ttttaaacat acgaccaata gaaaatatac
     8281 ctgttgaagc tcttggatat agaattgaat ttctggaaga atggttaaag agagtagaaa
     8341 aagatgagta ttgggatttg atggattggc atttacaaaa agataaaata aatgtacctg
     8401 cattggttgt atctgggtgg tatgatgata atagtatggg aactacagag gccttagatg
     8461 ttataaagga ctatgaaaaa ggaaagagaa aggctattct aggtccatgg atgcataatt
     8521 caaatacttt aagagatata aatggtattt ctttaggaaa tagttcatta agatatgatt
     8581 tggattataa ttatctatta tggtttgata aatacttaaa aggtatagaa aataacatag
     8641 atacgactgc tccagtagag tattactctg taggttctaa taagtggaag acagaagaaa
     8701 attggccaat aataaataag atagataaaa gtatgtatct aataagtgat ggaaatgcta
     8761 acacatcttt aggaaatggt agattagtat ttgataatga tttagaagaa aagtatgata
     8821 gttatatata taatccaaaa gacccatcag tacaactaat tgatatgtca gagaatgaag
     8881 ttggagttcc aaataactac aaagatttag aaaaaagaag tgatatgttg tgttatacat
     8941 cagatgcttt tagtgaagaa tttactgtaa caggtgatat aaagttagag ttttttgcat
     9001 caagttctgc caaagatact gactgggtta taaaaattat ggacgtggat ttagatggaa
     9061 attcaattaa attagctgat ggtatactta gtgctagatt tagaaatagt ttttataagt
     9121 cagagtttat ggaagaagga gagatttata agtttacgat tataacttca aaaatatcaa
     9181 acacgtttaa agttggtcat aaaattagac tagatataac gtctagtgct aaaaacttta
     9241 ttttccaaaa tagcaacacg acagagggat ataatagtat tgaatatata gaagcaaaga
     9301 atacgattta tcatggaggt aaatatccat ctaaattgat attgccaata gagaataagt
     9361 aaatttatat agattaaata gtgaggaggt gtctcaaaat gaatttttta gttcgtgaga
     9421 cactttcttt tatacaaaat caaatgtatt ttcatcattt caacaatgaa aaagaggctt
     9481 atctctctgt tttgagatag cctcctataa gctaaattga ttattttgta gctgcaacaa
     9541 cgctcatttc aactaacaag tgctctctag caagtctagc ttcaacacaa gctctagcag
     9601 gttcaaatcc tttttcaacc caagcatccc aaacagagtt catagcttca aagtctttca
     9661 tatctcttaa ataaatagta acagatagca aatgttgctt atctgaacca tatttgttta
     9721 ataaatcttc tattttagct aaaacttcag ttgtttgttc aattactcct ccttcagcat
     9781 gagtttgtcc acataaataa actgtattgt tatgaactac agctctactc attcttccag
     9841 ttccttcata tctctttatt tccatatttt aaatacctcc atagttaaaa aatcta
//
LOCUS       DAFKMX010000149         9698 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFKMX010000149 DAFKMX010000000
VERSION     DAFKMX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.149"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            129..1019
                     /locus_tag="LZB08_002872"
     CDS             129..1019
                     /locus_tag="LZB08_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBM7316360.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            1237..2523
                     /locus_tag="LZB08_002873"
     CDS             1237..2523
                     /locus_tag="LZB08_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBM7316361.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(2612..3055)
                     /locus_tag="LZB08_002874"
     CDS             complement(2612..3055)
                     /locus_tag="LZB08_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBM7316362.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(3079..3873)
                     /locus_tag="LZB08_002875"
     CDS             complement(3079..3873)
                     /locus_tag="LZB08_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBM7316363.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(4439..4915)
                     /locus_tag="LZB08_002876"
     CDS             complement(4439..4915)
                     /locus_tag="LZB08_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBM7316364.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(5016..6185)
                     /locus_tag="LZB08_002877"
     CDS             complement(5016..6185)
                     /locus_tag="LZB08_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316365.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKCNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVILHVKNVLSF"
     gene            6348..7211
                     /locus_tag="LZB08_002878"
     CDS             6348..7211
                     /locus_tag="LZB08_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBM7316366.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            7282..7875
                     /locus_tag="LZB08_002879"
     CDS             7282..7875
                     /locus_tag="LZB08_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBM7316367.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(8125..8760)
                     /locus_tag="LZB08_002880"
     CDS             complement(8125..8760)
                     /locus_tag="LZB08_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBM7316368.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(8840..9571)
                     /locus_tag="LZB08_002881"
     CDS             complement(8840..9571)
                     /locus_tag="LZB08_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBM7316369.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         3657 a         1168 c         1454 g         3419 t
ORIGIN      
        1 agttacttga catagtttac acttttaatt atagtataaa ttggaatatt tagataatta
       61 ttatttaatg tataagctaa gtttttaatt aatatggctt tatacattta tatagaaaag
      121 aggtacgtat gagtagcaca atcaaatcaa atcgaataaa aggtataatg tttataatag
      181 catcagcgct tggctttgca atgatgtctg cattcgtaaa gctagctgga gatttaccaa
      241 gttttcaaaa agtgttcttt agaaatctgg tatcagctat aatagctttt tggcttatca
      301 taaaacataa aggaagtcta actggtaaaa aagaaaattt aaaagtgctt ttatataggt
      361 caatatttgg aactctagga gttatattta actactatgc aattgacaag ttgatgcttt
      421 cagatgctaa tatgctaaat aaaatgagtc ccttccttgt agtaatattt tgtgcaattg
      481 ctctaaaaga aaaaataaat gtaaagcaga taggagctat tataattgca tttataggag
      541 ctttattcat aataaaacca acatttagtg tagaagtaat tccatatctt ggaggaattg
      601 caggagctgt atttgcagca atggcatata cttgtttaag agttcttgga gatagagaag
      661 attactatac aattgtattt ttcttttcag ttttttcatt agttactgta gggccaattg
      721 cttttgcagt atatgaacca atgaccctta tgcaacttat atatctatta ctaggtggag
      781 tatttgcaag tctaggtcaa tttggaataa ctctagcata taaatatgca cctgctaagg
      841 aaatctctat atttgactat agtaatataa tcttttcagc aattttaagt atattcttat
      901 tcaatgtcta tccagatatg ttaagtgttg ttggatattt aatagtattt tcggcagcat
      961 tttatatgtt tttatacaat aagaagttag ataaattaga caaagaaaag aagaagtaaa
     1021 ctagataaaa gtgtttttat aatttgtatt gaatatttaa gtctttgtat taaaattaaa
     1081 gaaaatttaa ttaaaaatgt ttggtatatt aacttataac agcagttaaa attgaaaaat
     1141 tataactgct gtttctttta ttaaaaatat ccaaatgtct gtaaaaatag actataatag
     1201 agtagtataa ataatataaa ggggataaat taaagtatgg aaaaagtgaa agttgctgtg
     1261 attggagcag gtaatagagg aacgtatgca tatgctccat atatttatga aaattctgat
     1321 gtatgtgaaa tagttgctgt agcagaacct aaaaaaggaa gacgagaact atttacacaa
     1381 aaatataacc tagatagtaa aaatgtattt gaaactttag aagacttttt taaacatgac
     1441 aagatggcag atgcagtaat aatagctaca aatgatgata gacattatga tgtagcaaaa
     1501 ctagctcttg aaaaaggtta tcatgtacta ttagaaaagc caatgtctaa tagtttagat
     1561 ggtttggtac atatagatga cttgtgtgat aagtataaag ataagatttt tatgatttgc
     1621 catgtactga gatattctcc attttataat aaattaaaag aaattgtaga aagcaaaaaa
     1681 ttaggagagc tagttagtat acaatataat gaaaatatag gatattggca ttttgcacat
     1741 agctttacta gagggaattg gagaaatagc aatgaaacca gtcctttaat tttagcaaag
     1801 agttgtcatg acatggatat tttgttatat ttagtaggaa gtagatgcaa aaaaatatct
     1861 gcatttggaa gcttaaagca tcttaataac caaaatgcta gtggagagat ggcacaaaat
     1921 tgtcttcagt gtttagttga aaaaaaatgt ccatattcag caaagagaat ttatttagaa
     1981 aaagatagaa gtataaatag agctgttcac ataaatccaa ctgaagagaa tctattaaat
     2041 atacttaaga caagtccata tggaagatgt gtttacagat gtgataataa tgttgtagac
     2101 aatatggtaa atatactaca gtttgaaaat ggagtgacgg ctacttttaa tttatgtgca
     2161 tttactaaag aaaatggaag aactataaag ttaatgttta gccatggtga agttggtggg
     2221 gatttaaata aaaatgagat aagaattaaa gaatttggaa aaaacgaaga aatagttatg
     2281 aatccatcaa atcaacagaa tatggtagag gaatatgata gaaatcttat agctgaattt
     2341 attaaattag tttcaaataa agaactagaa aagggtagag tagcagcaaa agaagctata
     2401 caaagtcatg taatggcatt tgcagcagaa tattctaggg tcagtgatga agtagtgtat
     2461 atagaagaat tttttgatag tgctaagcaa atgactaagg agatagaaga aactatattt
     2521 taattaataa aaatttatta aataaaataa agatagatta aataatagaa atacgaaaat
     2581 atgtgttcta ttaaggaatc tatctttttt attaaggttt tttcttatcc ctatcaaaaa
     2641 aatccataca ttctgataaa agataagaaa gtaaagcgaa taagatttct gtacttaaag
     2701 gaatcgttac tgaatccata aaggtatcaa ttatgttcat agctagaaaa gttagtataa
     2761 aatcaattat aaattcgcaa agtttatata ttgagtatgg taatttgttt acatatctga
     2821 atatatctaa aatagtagtg catagaaaat caattggagt ggttatacaa aaatatatta
     2881 aaaagaaaat taatacagct ccaagagatt tatattcaaa tcctaagaat gataagaagc
     2941 caattcctat aaccgtgaaa atacaaaaag ttataccaaa aaaaacagca aaaaataaaa
     3001 atacagaaat aaattcgcct aaaggaatcc ttcttttatt acttatctta atcaactcct
     3061 taatttatga tatgaaattt atataaataa taacattctt aaaaattcat aatagtcctc
     3121 tgtttcaaat agtactgttt gagaatctat ttttttcata tttggataaa aagaggcctt
     3181 aaaagcttta taatgttctc taaattttat ttctatctta aatttatctg gcaattttat
     3241 taagtgccta gataaatctc cagataaagc ttttttgaca cctttttcaa ttttttttag
     3301 tgcaaggttt ggatggattg aaattgaacc atttccaatt ccttttgata caggaacagt
     3361 gactatattt ggatttagtt tttttgcact ctcacaaagc atttcatctc cacttaaaaa
     3421 tgctacagga acattataat atgcagcaat ataagcattt atagtaaatt cagatgctat
     3481 ttcatcattt attttaaaat agtcataatc aatatccata gtatgggaaa gtggattacc
     3541 attacaagta gctccagagt gatagccagt gaatatacat gcatcaaagc tttcatcaat
     3601 tccagccatc ataactagtg ggtctttggt ccatcctcta agtattttga cgttttcagg
     3661 taaaatattt ggattaatat ttctaccact gtcatgtgca tctttaacaa gtatttcatg
     3721 tacaccagat tgagtagctc catcacatgc tgctgttatc tctttactca tttgtgttct
     3781 atagtgttgt gaaaattcac attcaagttt tgtttcatcc caatttacaa taccacaagt
     3841 accttctata tcagcactta aatacaattt cataatatat acccccttat gtttattatt
     3901 gatgataata acagataaca gaaaatacat aatcaataaa gggcataaaa atattatgtc
     3961 ttactattaa ttattattaa atctgtaact gaaaaataac taaattggta ttaaaaaata
     4021 taacactatg tgtcatttat ttatatagtt ttttatatct gatgtttata taaaattgta
     4081 acatataagt ggttacattt actagtttaa catagttaaa atagttaggt attattttta
     4141 agttaaattg attattataa aatcgcagaa taaaagaaaa atttaattta ttaaaatgca
     4201 tgaaatgttt tttcctcaac ataatgctaa attaaactca aaaagaaata atagaaaaat
     4261 cttatgtagc ttagaaagaa tgtgaatgat ttaagggaaa aatagaaatt tattatataa
     4321 tattaggctg tattatataa tacagcctaa tattatatgt atatgtgaca taaaatcttg
     4381 ctaagtgtaa ataaaataaa tgtggaaaac aagttaatga aatcaagaaa aaagtaattt
     4441 aaaattcaat ttcatcttcc caattaaatt ctcctgattt ttctacactc ttaggccagt
     4501 gactacacca tttaggaacg gacggatttt caaccctatc aacgcttgga gtatatggct
     4561 ttaagtcaat aacaggacta ttatcattag catcaatata tggtattttt attatgccat
     4621 tttcatggtc gatattgata atttgtacag cagtcaatgc tattggattg ggtcttatag
     4681 gtgaccttgt tgcaaatatt cctataacgt ctggtccact tttataaggt tttggggttt
     4741 ctaaaatact tcttagttca ggattatcaa acccatcagc ccaccaaaat acatttaaat
     4801 gactgaaatc gtcgagttca tttaatgcag gtacatactt tttgtctaat ttaataaaga
     4861 cctcttcgcc ttgaatacat attttaccta taggatttat taatatttct ttcataatat
     4921 tacctccaac tatttaattt atcatttgta attttagggt ataacatgac atcatgtgag
     4981 ggtcaatata aaataataaa taaaaatatt aatagttaga atgataatac attttttaca
     5041 tggagtatga caccagtaat aaggcaatcc tcgtcaatat caaaagtaga ggtatgcaaa
     5101 ggggaagtaa tgtttttttc acgatttgca caaccaaggt ggtaaaaagc tccttcacag
     5161 tgtcttagaa aatacgaaaa gtcttcagcc ccaagagtgg gcgagtcttt aaggataata
     5221 ttttcttctc caaacaattc tacagcattt tgtttgacgt aatctactag atggtttgaa
     5281 ttgataagag ctggataacc ttcctcaatt tcaacagaaa tacttcctcc aaatgtttta
     5341 caagtaaaat cgcaaatttc tctaattcgt ttttttgaaa actctctagt ttcaggagtc
     5401 aaggttctca aagtacccct tataaccact ttgtcacaaa ctatattttc cttgattcca
     5461 ccttcaatct ttcctagaga aataaccaca gaatcagttg gatttgtgtt tcgactaacg
     5521 attgtctgta gagctgtaat aatatgagca gatataacaa tagcatcaat accttgatgt
     5581 ggataagctc catgacattt tgaaccatgt acagttaatt gaagtgtatc ggtggaagca
     5641 tttagagtgt tgcattttaa ttcaattaga tttttgttga tattgggatt tacatgaagt
     5701 ccaaatatat attttacatt tgggttttca agacatccat cttctatcat aagttgagcc
     5761 ccaccaatag tttcctcggc aggttgaaaa aagaatttta catttacatt taagtaatcc
     5821 tttaatttaa atagtatatt acaagtaccc agtaaaatag cagtgtgtgc atcatgacca
     5881 catgcatgca ttttgccaat attagtagat ttataagaat aattaagttc ttctgttata
     5941 ggtaaggcat ctatatcagc tcttatagca actgtttcaa agtttggact cacgttaatg
     6001 tatgcagtta tccctgtatg attcttatat tcaatataat ttatacctat ttcatttaag
     6061 tatttaataa tttttttctt agtttgaaat tcttctaatc caagttctgg agtcttatgt
     6121 aaatctcttc ttatatttat aacccaatca tttatctcat gacaaagagt ttttatatta
     6181 agcataaaac acatcctaac tatattaaaa atttattttt cttcattaat tatattattt
     6241 tatgtaaatc attattatgt atcaagtaca aataaaatat cttataatta tattagagat
     6301 atttagtatg gagaagaata tatcatttta taagaagagg tgtttaaatg ttaggaataa
     6361 ttgcgccatc tgggccatta agaaatacaa gtttggagga aataaagttt aatttagaaa
     6421 gttatggtta cgaagttaag ttttctgaaa gttgtagttt aaattataaa ggttatttag
     6481 ctggtaatga tgatattaga gcaagggata tagaagatat gtttttagat aaagatgtag
     6541 atataattat gtgcttaaga ggaggttatg gaacaacaag aatattggat aaaataaatt
     6601 atgatataat aaagcaaaat ccaaaaccat ttataggttt ttctgatata acaggtttaa
     6661 atttagcttt ctataaaaat tgtggtcttt taccatatca tggaataatg gcagcaaatg
     6721 taggtaaatg ggataaattt acctataaat cattggtaaa tgctttagaa tttaaagatg
     6781 aattatattt agaaaatcca aaagaagaaa agatttatac tgtatgtgaa ggtaaagcag
     6841 agggaataat aatgggagga aatttatcct taataattgc cacaatggga acaaaatatg
     6901 aaatagatgc aaaagataaa attttgttta ttgaggaaat tggagaacct caatataaat
     6961 tggatagaat gttaacacaa ctatattctt caggaaaact tgaggaatgt aatggtataa
     7021 tttttggaga ttttaaggat tgtattgaag aaaatgattt gatggaatta ctaatagaat
     7081 ttgcaaataa ggtcaataaa ccttctatat ataatcttca gtcaggacat tgtatcccaa
     7141 tgataactat tcctttgggc agaatgtgcg aattagatgc tacagaaaaa atcgttaaat
     7201 taaaaaaata gtgtgtatat aaatataata gaaatatggt ataatcttat acaagtaaaa
     7261 atttagctag gaggatgaaa aatgtctgat gatagaataa tagactttaa cgaactaaag
     7321 aacaaagtta aagattctga tgtagataaa tttgagcaat acatatataa cttatatttt
     7381 tctgttatgg atggaaagat gtctatggct gagttttcaa gaaaaatatt cgattatatg
     7441 agagataaca acatatctca agaaaaattt atgaaaatac aaaagcaatt tatggagaga
     7501 tatggtatgg acactgaaga agtagaaaaa caacttagaa actttggaat cgacccttct
     7561 acagcaggtt ttatgagtaa taatacttcc agcaaagtat ctacagaaga tttagagtca
     7621 tttaagaaga gtgcaggatt ttatgagaaa tatggagaaa aaatccaacc aaagagttgt
     7681 ataacaacat ttataaaaaa tgacctaaat gatattaatg taataatcga ccaagaaaag
     7741 ataatgcttt gtagtgatag aaaaataaat cttatggatt cagaactaaa tgagttttta
     7801 ttagaatata agaatatgtt taataaaaaa ataaaggtag taatgtgcga aactacgaat
     7861 aagtatgatt attaaaaaat gaatttaaat actgaagtag tatcaattaa ttgtactact
     7921 taaaaataaa aatcgcaatt tattattcct tataaatttg tataaggaat atctaaactg
     7981 cgatttttta tattgtgaag attagtcata atagatagtg ttttaaattg taatcttctc
     8041 actaaaactg tgtttttcta ttagaaattt tattaaaaat aaatatctga taattgtatt
     8101 atatggatgt aaagtattag aggcttaacg aatctctttt aatacattaa ctccattttg
     8161 aatcatactg ttgaaaaata ctaagtttaa aatctcagca gggtgaacgt taggaatatc
     8221 ataagtatct actaaattca taggtacaac tatatttttt tcaatattat gttgattaaa
     8281 atatgatttt agagttattg caaattgata tatacatata tctgtgcaat cgccaactat
     8341 aattatatta tctatattat caaaatccaa attttctaat gcaaaaaatc cattagtaga
     8401 atttttagga agaatttgta ggttttctat cgacttaagt tcatcaacca attcactttc
     8461 tacatcattt tccaaacagt gaacaggata actcaaaagt tctattgatt taggagtatg
     8521 agaatcagta aatgctatta ctctatttaa tttgtttgat attttttttg taaacatttc
     8581 aattggcttt attaaagact ctactctagg agagtataat gcaccttgtc ttgcaaagcc
     8641 attattaaca tcaattataa ataaagcagt tttactcaaa tcataatttt ctattttttc
     8701 tattggtaga ttatctaaat tactctttaa agcttcaagt tcatttaata agttatccat
     8761 agttatacct ccaagataaa tttaataaaa ttgtacatat taattatata aaagtcaagg
     8821 agtacaaact attgtataat tagctgtatt gttttgagtg taaaagattt gaagcaatat
     8881 ctggatagtt agtaaaaata ccatctacat tgagttttag acagtgtagt atatcatcct
     8941 cttcatttac agtatataca ttgacaccaa gtagattttt gtgacaaagt tcaattagag
     9001 attcatttac taaaactaga ggtggatgaa gatagctatt acccagaaat ttagcaaaat
     9061 tgactacatt gtttataggt aaataacata ataatcctgt ttttatacta gggtctaatt
     9121 tatgaaagtt ttttaaacta ttgctgttga aactagatat aagaactcta tttttcatat
     9181 tatactttaa aataagctct aaaacgtctt tttctatatt gggatagttt attttgtcag
     9241 tttttagttc tatatttaaa ataaggtctg taggtgaaat tagaattaat acttcttcta
     9301 aagttggaat tttacattct ttattgcttt gatatccttc aaaagatgag tttagagttt
     9361 ttaattttct taaagtaaag tcttttacaa aaccatgtcc attaaaagtt ctatcaacct
     9421 tttcatcatg aataactaca atttttccat ccttagttct gtggacatca agttctattc
     9481 catatatatc catatccaaa cattttttaa aagctagaat agtattttct ggatactttc
     9541 cactaaaccc tctatgagca taaatattca tttatatggt tccctcctat aagtataact
     9601 tatcttataa aaatttaatt aatataatat atcatattcc aaaaggtatt gttatatatt
     9661 agttaaataa ctatttgtaa tttatatatt atataaat
//
LOCUS       DAFKMX010000150         9628 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFKMX010000150 DAFKMX010000000
VERSION     DAFKMX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.150"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            174..1214
                     /locus_tag="LZB08_002882"
     CDS             174..1214
                     /locus_tag="LZB08_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316370.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1570..2892
                     /locus_tag="LZB08_002883"
     CDS             1570..2892
                     /locus_tag="LZB08_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBM7316371.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3189..4550
                     /locus_tag="LZB08_002884"
     CDS             3189..4550
                     /locus_tag="LZB08_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBM7316372.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAADLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4623..6017
                     /locus_tag="LZB08_002885"
     CDS             4623..6017
                     /locus_tag="LZB08_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316373.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6079..6549
                     /locus_tag="LZB08_002886"
     CDS             6079..6549
                     /locus_tag="LZB08_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBM7316374.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6552..8768
                     /locus_tag="LZB08_002887"
     CDS             6552..8768
                     /locus_tag="LZB08_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7316375.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8851..9522
                     /locus_tag="LZB08_002888"
     CDS             8851..9522
                     /locus_tag="LZB08_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7316376.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
BASE COUNT         3608 a         1131 c         1933 g         2956 t
ORIGIN      
        1 tgtaaaatga agaaagtata tctatcaaat taaaactaag tagatatact ttttattaag
       61 ttttgtttta aaataatatt attaaattat aactaagctt ttctgttatg atataattaa
      121 atagataatg tgaatgatta ttatatagaa tacttatttg gagttgatat tttatggaca
      181 aacacagtga ttcagtaatg tatggtggaa tggaagtata taatatttgg ggaacaacaa
      241 cagtagactc cataattgga gagtctgatg taatgcgtgc tcttaagaat agaatttgta
      301 aaattggaaa tagtgattct actgtagcta taactggaga aagtggcact ggaaaagaat
      361 taatagcaag agccattcat ctcgcaggaa acagagggaa taaaacattc gttgctataa
      421 actgtgctgc tataccagaa cctttactag aaagtgaact ttttggatat acaaaaggag
      481 cttttagtgg agcagattca aaaggtaaaa ttggtaagtt tgaattggcc aatggagggg
      541 ttgtattttt agatgagata ggagatatgc caattcaact acaatcaaag ttattgaggg
      601 tattacagga aaagaagttt acgagaatag gttcaaatga actaattgat atagatgtta
      661 gaatcatatc agctactaat agagatttat ttgagttggt taaggaaaat aaatttagag
      721 aagacttata ctatagattg aatgtaatac ctttagaagt gcctccactt agagaaagag
      781 gagacgatat acaaatatta ataaacaatt ttatagacaa atattcgaga aagatgaaga
      841 aaaacgtgta ccatatagag ccagatgtgg aacaaatgtt gcttaagtat ccttggccag
      901 gaaatattag agagttggaa aatactatag aactggcatt aaaccttctt gaggaagatg
      961 gaataataca taaaggaata ataaatagaa agatacttga atattttaag ttaaataata
     1021 taaatataaa gttacttgag gaaagtgact atgagataag tatggaacaa gacatactta
     1081 cacttgaaga gtttgaaaga gcctatataa agaaagtgtt aaagttttac ggaaatgata
     1141 ctaagtctaa aaaactggct gccaaaaaac ttggaatatc actagctaca ttgtacagaa
     1201 aaatagatgt ataataatta ggctttttat tctcaattct aaaatttgaa attataaaat
     1261 tgataaaaaa cacttggaaa actattttct aagtgttttt ttgtgtaata gcattttatt
     1321 atccaaaaat aaaaaaaatt taaaagaatt taaaaaaata aatgctttaa taaattatca
     1381 aatactaaca aatataataa aaattatata tgtaaaacaa ttatgttaaa aaattggcat
     1441 tataattgct tcatattaag ttgagatgac aaaataaaaa aagaaaggat gttgtgtgag
     1501 ataataataa ctgtcattaa aaaatagttg ggagtgataa ccttgagtaa atatcaaaag
     1561 aatgtttcta tgtttgatat tgatggaagg ccaccaattt taaagtgttt gccactatca
     1621 atgcaacata tactagcaat gatagttgga actgttacag tacctatagt cgtaggagga
     1681 gctgtgggtg ttagccctac acaaataaca cttttagtgc aatatgcatt aataatagct
     1741 ggtatatcaa cacttataca actatatcca ataggaaata ttggttcaag attgcctata
     1801 atttttggag ttggttttac ttatgtacca acacttgtag cagtcggagg aaattatgga
     1861 ttagcaagca ttttgggtgc acaattaata gggggagtgg ctacagtatt aatagggata
     1921 ttcataaaaa agataagaaa attttttccg aatatagttg caggaacagt ggtttttaca
     1981 attggattat ctttgtatcc tatagcaata aattatatgg caggtggtgt aaatagtcct
     2041 acttatgcct ctatgcaaaa ctgggcagta gcggctataa cattagtggt agttataggt
     2101 cttagtcaat ttgcaaaagg atatgcaaaa ttggcagcaa tatttttggg aattatagtt
     2161 ggatattgtg tatctttagt tctgggtata ataaattttg accctattag agaggcaact
     2221 tggtttgcga tacctaaacc gcttgaattt gggatggaat ttgatatgcc tgtaataatc
     2281 tcagtagtaa ttgtaagtat aatcaatgct gttcaagcaa ttggagattt atcagctact
     2341 acaatgggag gcatggatag gaatatcaca gataaggaat tatcaagtgg agtaatagga
     2401 agtggaattt gtactatgat aggagctttg tttggaggtt taccaccatc atcatatagc
     2461 caaaacgtgg ggatggtagc tatgaacaaa gtaataagta gatttgtgct tggaatagct
     2521 ggtatattta tgttattgtc ggggtttgtg cctaaatttg gtgcattaat gactactata
     2581 ccatattctg ttttaggagg agctacaata tctatattta gtatgataac aatgacaggt
     2641 ataaagttaa ttattacaga agagttgtca tcaagaaata ttaccattgt tggtttggca
     2701 attgccttag gcatgggaat aacatcagta ccagcagccc aagaggcatt tccttcattt
     2761 gtaaagttag tatttggaga atctccaata gtaattgcaa cattggttgc attcgtttta
     2821 aatctaataa taccaaataa aagtctagag gatgaggcta aagaaagagc tgttatggaa
     2881 gagactaatt aagatataat aatttaaaag aattgtttaa attatttagt atgagattgt
     2941 tagattattt ttgatagcta ttcattttaa taaaaaattt aacaaataaa tatataccag
     3001 gcaattaatg ttcatttaag ttgaaaatcg gggagggaat atttagaaat ttgttaattt
     3061 tttataaaaa tagctcagtt tagctgtgtt tatcggttga gcaataaatt catggtcata
     3121 aaaatattta tagaaaataa ttataaaaat atttagtcaa gatatttatg tatgggagga
     3181 tttattttat gaaaacacgt aatacttcaa aatatgatgt cgatgggata ccaccactaa
     3241 aggaatcaat acctttggcc ttacaacatc tgttagcaat gatagtagga aatatggtac
     3301 ctgcgatatt aatagcaaac gtggtaggac ttaaccaggg gcaagcaaca atgttgattc
     3361 agggttctat gttagcagca gatttagcaa cgtttttaca gttatatcca atacctttat
     3421 ttaaaggatt taagttaggt tcaagattac ctgtaatgat gggaatgagt tatgtatttt
     3481 taggagcttg tctttcagta gcagctaaag atggtttagc agctctattt ggagcacaga
     3541 ttgctgctgg ggtaatcgta ttctttgtag gatttggagt taagaaaatt agacatatat
     3601 tcactccaat agtttctgga actataatag catgtatggg acttggacta ttcgcaacag
     3661 ctataaagaa tttagctgga ggagaaggca cacaaacatt tggaagccca ataaacttca
     3721 cagtaggagt tatagttgct tttgtaataa taatgataaa taaatacggt aaaggtttag
     3781 taaaaaattc ttcaatatta ataggaatat tagtaggtta tgctatatct ttagttttag
     3841 gattggttga tttctcagcc gttcaaggag cagctatagt atctttacca acaccagcag
     3901 cttttggatt agaatttaga ccagagttaa tagtaatgtt tactattatt tacataatag
     3961 gtatagctga tatgatgggt gcctgtacaa tagctacagt aggagcaatg gatagagagg
     4021 taactgacga agaattagca tcagttgttt taggaaattc tataacttct ataatttcat
     4081 cactatttgc agctcttcca actggagtat ttagccaaaa tacagtaatt gtatctatga
     4141 acaaagttac aagtagattt gtaatagcac taggggcact tgtattatta ctagcaggga
     4201 tatctccagc attgggtgca ataatgacaa ctataccatc ttgtgttgtt ggtggagcaa
     4261 ctttagtagt tttctcatca atagcaatgt ctggtttctc tattatgagt atggatggat
     4321 tcactgaaga aaacaatctt atagcaggtg tatctatagc aacaagtatg ggtcttacaa
     4381 ctgctccaca agttttagac caattcccag aaacaattgg aactgtcctt ggagggtcaa
     4441 gtatagtttc tggagcaata atagctatat tacttcaaac attatttaaa ttaaagtcac
     4501 gtaaaagtgc tgaaaacgtt acttcaaatc ttgaggaaaa tattggataa atgtattaga
     4561 tataatatga gaaaaccata tggtaaaatg ttgtaatgaa agtattcaaa gggggattta
     4621 agatgattga tatactaata aaaaatgcaa taattgttac agttaataaa gacagagatg
     4681 ttatatttga tggagcaata gctattcaag gtgacagaat cttggacata ggaaatacag
     4741 ttgatatcga gcctaagtat cttgatgcta aaaaagttat agatgctaat ggaaaagtaa
     4801 tattccctgg ctttataaat actcataatc acctgttcca agtgttatta aaaggtttag
     4861 gtgatgatat ggcacttcat gagtggttaa atacaatgat gtttccatca gcaaaattct
     4921 taacagaaca agatacttat gatgctgcaa tgcttggatg tatggaaggt ttaaaaagtg
     4981 gtataactac aatggttgat tatatgcata ctcacaatag accaggactt acagatggaa
     5041 tagtaaaggc atataaagat ttaggcataa gaggggttgt aggtagaggt tgtatagatt
     5101 taggtattca caaagagctt atagaagatg tggaaacggt tgaaaaagat ttgagaaggg
     5161 tattcgaaaa atatcataat tcagaaaatg gaagaataaa aatctgtgta gctccttctt
     5221 caatgtgggc tatatctgaa gaaatgggta agatgctttg gaatatagtt aaagaatatg
     5281 attcatattt tacagttcat atatctgaaa cagaatttgc aagaacagca acaaaggata
     5341 tacatggaga aatagatata aaattacttg aaaaatgggg tatagtaggt ccagaagttg
     5401 tagcagtaca ttgtgtatgt ataactgatg aagacatgga aatgcttaaa aagtatgaca
     5461 taaaagtttc tcataatgta gcaagtaata tgtatcttgc ttctggtgtt gcacctgtgc
     5521 cagaaatgtt aaaaaaaggt ataaatgtga gtttaggtct tgacggtgca gcaagtaata
     5581 acgcacaaga tatggtagag cttatgaaat taacagcttt acaacacaaa gtaaataatt
     5641 gtgaccctct ggcaatttct gcagagaaag ttcttgaaat ggctacaatt gagggagcaa
     5701 gaactcttag aatggaagat gaaataggtt ctttggagat tgggaaaaaa gctgacctag
     5761 taatatttaa ctcaatgctt tctccaaaat caatacctct tcacaatcct gtatcaactt
     5821 tggtatactc agctagtatg cacaatgtag agagtgtttt agttgatgga aatgtaatac
     5881 ttgaaaatgg aaaagtaact acaattgaaa gtgaagaaca agtgttgctt aatgcacaac
     5941 atgcagcaga agaattatgt aagagagcta atgtaactaa tagaatggaa ggacataagt
     6001 ggaatagctt atactaatat taaaataaga ttgtaataat attctattac aacattagtt
     6061 tatctgtagg aggagaaaat ggaaaaaata aaattaaata ttaatgatta catgtatgaa
     6121 atagaagttg aaaagaatga aactcttttg catgtacttc gtgaaaaatt aaatctaact
     6181 ggagcgaaac aaggttgtaa tagtggcagt tgtggagcat gtaaagtttt gatagatgga
     6241 gttgacatga aatcttgtaa acttcttgca aaaaactgcg aaggtaaagt tatatacact
     6301 atagaaagtt ttgctaatgg agaaaatctt catccaatac aagaagcttt tatagaagtt
     6361 ggagctgtac aatgtggata ttgtactcct ggtatgatta taacaacaca agctctttta
     6421 aatagaaatg catctcctaa tgaagatgaa attagagaag cttttaaaga aaatctctgt
     6481 agatgcacag gatatgtaaa gattatagaa gctgtaaaat tatctgcagc tagattaggg
     6541 ggtagataat tatggaaaag gtaataggaa aatcataccc tctaaaagat gcagctttaa
     6601 aagttactgg tcaaatgaaa tacgtggctg atatgaaacc acaaaatgta ctttacgcta
     6661 agatgctttt aagtcctgta gcacatgcaa aaataaaaag tatagataca agtgaagctg
     6721 agaaagtagt aggtgttaga gctatagcta cttatctcaa tacatcacaa agaaaataca
     6781 atggttcatt aagattttat gagcacaaaa tacctgaaaa tgaagttata ttttcagata
     6841 cagttagatt tgttggtgac cgtgttgcag cagtagccgc agagactcca gaaatagcag
     6901 agaaagctgt aaaacttata aaagttgaat atgaagaatt gccagctgta tttactatac
     6961 aagatgcaat aaaagaagat gctatctgca tacatggaga ttcaaatata gtttctcaaa
     7021 acaacataaa tggaggagat gtagaaaaag gcttcaagga atgtgactat atatttgaag
     7081 atgaatactc tactccagca atacatcata gtgcaataga gactcatagt gtaattgcac
     7141 actatgatta taatgataaa ttaactgtac atacaccttg ccaaaataca tttggattta
     7201 gaataatact atctcaaatt tttgggatgt cattaaataa agtaagagtt ataagacctg
     7261 ctattggagg ctcatttggt ggaaaattag aacttacaat tgaaccagta gcagccgcat
     7321 tatcaaaaat gacaagaaga ccagttaaaa tggttcttac aagaaaagaa tctatgatat
     7381 cgacaagaac tcgtcatggg tcttttgtaa aattaaaaac tggtgtaaat aaagatggtg
     7441 aagtaatagc acaagacata aaaatatata caaatacagg agcatatgca tcaagtgctt
     7501 taaatgttat aggagcactt agtcataaag tatttaaagt ttataaaata ccaaatataa
     7561 agtttactgg gatgcctgta tatacaaata caccaatagc aggagctatg agaggctatg
     7621 ggtcacctca aatatttatg gctcaacaag ctcaatttgc gaaaatagca aaggaaatag
     7681 gtatggattt agtcgatttt caaaataaaa atgcagtaga gccagatgat gtcgatataa
     7741 tcttccacgg ttctcttgga aatcctagag tattggattg tatagagcaa ggtaagaaga
     7801 tgtttaagtg ggatgaaaag aaaaaacttc caaaagaaga aggaaaatat ttaagaggta
     7861 taggtatggc aataggtgca catggtaatg gagtttttgg tgcacataga gatgttgtta
     7921 cattgactct taaattaaat gaagatggta cgttgacact actaacagga gctcatgata
     7981 tgggtaatgg tgttgtaact atgcaaacaa tgatggtagc tgaagtttta ggaataacac
     8041 ctgataaagt tgatacattt gaaactgata cggatgcatg tacattcaac cttggtgact
     8101 atgcaagtag aggggtattt gtagaaggtg gaggagctaa aaagacagca gagaaattga
     8161 aaaatcttat attagaagaa gcagaaaaat tacttgaaac atcaaaagaa gatttatatc
     8221 tagacaatgg atgtgtaatt agtaaacttg atgaaaatgt aaaagcatca ctttctgatg
     8281 tcgcagtata tagtcaaagt aaatcattaa gagagttaac tgttgttgaa gattattctt
     8341 caccagctgg acgtacttct tatggtgtac attttgctga agttttagta gataaagaaa
     8401 ctaaggatat aaaagtagtt gattttgtag ctgtacatga tgttggtaga gttataaatc
     8461 ctctaaattt agaaggacaa ttagaaggtg ggattcatat gggtctagga tacgcattga
     8521 gtgaagaatt ggcatttgat gaacaaggta gacctaaagc tactgatttt aaaacatata
     8581 aattatttag aactgtagat atgccaaaat gtcaagttgc atttgtagat ggttatgaaa
     8641 aagctgggcc ttttggtggt aagagtattg gagaatgtgc cgttgtacca gttgcgccag
     8701 ctgttgcaaa tgcagtatat aatgcaactg atgtagagat acattcatta ccaataaaat
     8761 taaaatagta gaaattaaag cagaaataac aatttattgt atttgtaaat agataataaa
     8821 aatagattta tttggtgagg agatattatt atggcaaaga tattaggtat ttgtggaagt
     8881 ccacgtaaag gagcaacaga atatgcagtg ttagaagctt tgaaagaagc agaaactata
     8941 cctggtatag aaactgagta ttggtcagtt agagggaaaa aaataagtcc ttgtgtacat
     9001 tgtgatgcat gtataagaaa agaaactatg tgtattataa aagatgatat ccaagaatta
     9061 gagcaaaaaa tattggaggc agatggaatt atagtaggtt ctccagttta tgatatgaat
     9121 ataactgctc aacttacagc agtatttaat agacttcgtc caatgtattt agttcatcca
     9181 ggaaaacttc aaaataaggt tggtgcagct ataactactg gtggaactag atatggtggc
     9241 caagaattga caaaacttcc tataataaat ttcttcctaa tgcatgaaat gttagtgtct
     9301 ggaggtttag gtggatgcta tataggtggt acaatatgga gtaaagatgg aaaagcaaaa
     9361 ggtgcccaag aggatgagac aggtatggac acagtaaaaa gacttggaaa aggtgcagca
     9421 gaggcagtta tggtttctaa gtttggtctt gaaaaatgga atgtcgtaaa agaagaatta
     9481 gatgttaaaa aagatgaaaa atcacctctt agagaccact aaagatttat tatattattg
     9541 aatatataca aatagtgatg taatagatta ttgcatcact attttatgtt ttaatttaga
     9601 ttttataaat tttatattaa aaaatata
//
LOCUS       DAFKMX010000151         9595 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFKMX010000151 DAFKMX010000000
VERSION     DAFKMX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.151"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            25..759
                     /locus_tag="LZB08_002889"
     CDS             25..759
                     /locus_tag="LZB08_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316377.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTREKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            970..2151
                     /locus_tag="LZB08_002890"
     CDS             970..2151
                     /locus_tag="LZB08_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBM7316378.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            2135..2707
                     /gene="mobB"
                     /locus_tag="LZB08_002891"
     CDS             2135..2707
                     /gene="mobB"
                     /locus_tag="LZB08_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBM7316379.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            2826..3101
                     /locus_tag="LZB08_002892"
     CDS             2826..3101
                     /locus_tag="LZB08_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7316380.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3344..5044
                     /locus_tag="LZB08_002893"
     CDS             3344..5044
                     /locus_tag="LZB08_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316381.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5407..6594
                     /locus_tag="LZB08_002894"
     CDS             5407..6594
                     /locus_tag="LZB08_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316382.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6654..7817
                     /locus_tag="LZB08_002895"
     CDS             6654..7817
                     /locus_tag="LZB08_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316383.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            7968..8771
                     /gene="zupT"
                     /locus_tag="LZB08_002896"
     CDS             7968..8771
                     /gene="zupT"
                     /locus_tag="LZB08_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBM7316384.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            8866..9426
                     /locus_tag="LZB08_002897"
     CDS             8866..9426
                     /locus_tag="LZB08_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBM7316385.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         3728 a          953 c         1761 g         3153 t
ORIGIN      
        1 aatttaagaa aggaggattg atttatgaag ttaaaaaggt cattagtttg tgtaactatt
       61 ttaggtatta tacttgttgg atgtcataaa gaaaatacta gagagaaaaa tcaagtagca
      121 agtaaagcaa cacagcaaaa aactatgaca aaagttcaaa atgatgttaa tgaaattatg
      181 aataaagatt ataagtacat tataaagaat atgggtatac cttataatac attttattat
      241 ataaaaccta aagttttaaa agaatcaaat acaatgcaag atataaatac atcaagttat
      301 atgacattag tttatccaaa atatactgga aatgatgaac tagatggaag tgctttatat
      361 gttgatatta acgaaaataa agttgtcaat gttgaaacca attctttctc atcacaaggt
      421 atatcagtca ttgatgctga atctagtata gtgattgaaa aaagtgacca tgaaaaatcg
      481 gctgtatcac tagaaaattt tagacatatt gacctgggag agtatgttgg tgttgaagat
      541 tccagaataa atgaaattgt aggagatgcg aattatgatt taacagcata taatcatgaa
      601 ggcagtaaag tagttaagag ttatagattg aaagaagata acaaaatatt aaaaaaagag
      661 gtacttacaa taagtattgt ggataacaaa ataaaatcta taaaaactat tgaaagtgat
      721 aaaattgtaa aaataataaa aggaacttta ttagaataaa atattctaaa gcatatgatg
      781 ctgtttcaag atagaaggaa atttctagct gggaacagtt tttttattag atttataaaa
      841 tttgaactgc acttatagat aactattagt tataattaaa tataagataa aagtatttcc
      901 aacttttata atctataata tattacatag aaatatctta aataaaaaat atattgggga
      961 gtatttaaaa tgatagaatt agaagatgcc ataaaaatta ttgaaaaaaa tgttttacca
     1021 ataaacaggg tcaagatagt aaatcttata gatgcagtaa ataaggtagt tgcagaagat
     1081 atatattcaa caattgatag tcctccattt gataggtcac cctatgatgg ttatgcatat
     1141 aatgcaaatt ctgaaaatag aaatttaaag gttataggga ctttgtatgc aggagatgta
     1201 tttaataggg tattaaataa aaatgaagca gtgaagataa tgactggtgg aaagatacca
     1261 cagggtgcaa attgtgtaat aaaaaaagaa gatgtaacag ttataggtga tatgataact
     1321 ttaaatataa aattaaagga atacgataat tacatattta agggtgagga tataaaaaag
     1381 gggcaattga ttattaataa aaatcagaat ataggatatg atggtattgg agtattagca
     1441 agtcttggaa tatccaaagt aactgtttat gatgatttaa aagttggaat tttgaataca
     1501 ggtacagaac tacaagatat aaatgagact ttagagaatg gtaaaatata cgatagtaat
     1561 agatatacct tatattcaag attattaaaa cttggaatac aagcagatac aatatgcaat
     1621 tgttctgatg atatattgga gttagaaaaa aaggtaaaga atatgttaca gagtgtagat
     1681 tttttaatta ccacaggtgg tgtttctgtt ggagataaag atttaattcc agatgtattc
     1741 aaaaaaatag gggcaacaga attattttgg aagataaaca ttaaacctgg tggagcttgt
     1801 tatgtaggta cattgggaga aaagctatta tttggtcttt ctggtaatcc acatgcagca
     1861 atagtggtat ttgataatgt gatagttcct gttattgagt atttaacata taagaaggga
     1921 acacatcata taaaagctat atttaaagga gaatttaata aactttcaaa taaagataga
     1981 tttgtaagtg gaaaactata tacaagtgaa ggtaaattgt atgtagagct aaatgataag
     2041 aaagatgcaa aagctagatt atctgcaact ctaagaagta attgtatgat taagattaaa
     2101 aagaatgaga caataaaaga aaatcaatta gtggatgttg ttattagata gttttatgaa
     2161 aggacacaat tttatgtata atatattgtc agttgtctca tattcaggag taggtaaaac
     2221 tacgttgatt gaaggcataa taaaagaatt aaataaaaaa gggtatcgtg ttggagttat
     2281 taaacatacc tgtcatgatt ttgatatgga tgaggaagga aaagatactt ataaacatag
     2341 aaaatctggg gcgagaaaag tctgtataat atcagataaa cgagtagcat atattgaaga
     2401 actaaaagag aagaatccat tatataaaat gattcaatta tatgaagata tggacttgat
     2461 tatcatagaa ggatataaaa attatagatt taaaagattg gaagtaacta gaaaaggtaa
     2521 gtatgaggat attttatcta ataagtctga tttgataggt attgtcagtg atatagaata
     2581 taaattgaat attgaacagt ttaatttaaa tgattataaa aatatatcaa agtacataga
     2641 gtcttgtata aaaaatgaaa ttttaagtat aagcaatgtt gaaataaaaa acatattaaa
     2701 agaataattt taagatgttc ttcttatata aaatgatata attatttata taaataaaga
     2761 tataagaaat ttatcaattt tattttgagt tataaaatat taaagaaatt gtaggaggac
     2821 aatatatgaa aaaaatatta gtagcctgtg gagcaggaat cgcaacatca acaatagttt
     2881 gtgacagagt agaaagattg gttaaagaaa ataatataca agcagaagtt gagcaatgta
     2941 aaattgctga atgttcaaca aaacaagaag gtgctgattt aatcgtatct actactatac
     3001 taccaacaac ttatgatata cctacaataa aggcaacagg atatataaca ggtataaata
     3061 caacagcatt agataaaaag atattaaatg cattgaagta gttttgtatt tataaaaaaa
     3121 atttagtatt aaatatagag ttgtatcttt ataaaatttt aaatatagag atatagctct
     3181 ttttatgttg tttcaagtat acaactatat gatataaatc ttcaatttta tgaaaaagat
     3241 tgttattact ttgtaagttt ataaaagatt taaagttatt gttatgttat actaatttat
     3301 gggtattttt tagaagtact aataaaggag ggatttaata cttatgataa aacaatttgt
     3361 caaatattat aagccatata agaaaatttt tacactagac cttatagcag cttttctatt
     3421 ttccttatgt gatttagttt atcctatgat aactagaaat ataatggatg acgttgttcc
     3481 aaataaaaac ttaagaatgt tagtagtttt tgcagtagca cttatactta tatttatagc
     3541 aaaagcaggg cttaactatt ttatgcagta ttggggacat gttattggtg tagatatgca
     3601 agctgatatg agaaatgagg tatttacaca cttacaaaga cttccaaata cgtattttga
     3661 taataacaaa tctggagtaa ctatgtctag aatagtaaat gatttaatgg atataacaga
     3721 acttgcacat catgggcctg aagatttatt catttctata gtaatgttgg ttggttcatt
     3781 ctttatactt atagatataa atattcctct tacgcttata atatttgcta tattaccgtt
     3841 tataatttgg tttgcaatag ctaaaaaaga taagatgaat attgctttta tgaagagtag
     3901 agttactata ggtgatgtaa atgctacttt agaaaatagt atagcaggta tgaaggtaac
     3961 taagtctttt tgtactgaga aagaagagtt aaataagttt gtaagaagca ataaattatt
     4021 tagaagagca agacaagatt cttataaggt aatggcagaa tattactctg gtatgaattt
     4081 atacatggat atcttagagt gggtagttgt aatagctggt ggttatttta cttatatagg
     4141 aaagattact ttgggtgatt ttgctgctta tatactatat gtaaaaatgt ttatacaacc
     4201 tatgaaaaaa ttaataaact ttacagagca atatcaaaat ggtatgacag gttttaaaag
     4261 attcatagag ataatggaac aagaccatca aaaagaagct aaaaatccta ttgaacttga
     4321 aaatgtaaaa ggtgacattg agatagaaaa tatatctttc acatatgaag ataagactca
     4381 agttttggat aatttaagtt tatctataaa agctggtaag actatagcat tagttggacc
     4441 ttctggagga ggaaagacta cattatgtaa tctattgcca agattttatg aatttgataa
     4501 gggtgatata aaaattgatg gaaagagtat aaaagatgtt agtttaaaat cattgagaaa
     4561 aaatataggt atagttcaac aagatgtatt tttatttaca ggaactataa gagataatat
     4621 tctttgtggg aaccctaatg ctacagatga agaaatgata gctgctgcta aaaaagctag
     4681 aatacatgac tttgtagaga ctttgccaga tggatatgat acttatatag gagaaagagg
     4741 cgtaaaatta tcaggtgggc aaaaacaaag aatttcaatt tcaagaatct ttttaaagaa
     4801 tcctccaata ataatattgg atgaggctac atctgcttta gacaatgtaa cagaaagaga
     4861 aattcaagag tctttagagg aattaagtaa agatagaaca aatttagttg ttgcacatag
     4921 attgactact ataaaaaatg ctgatgaaat catagtattg acggataaag gaattgaaga
     4981 aagaggtact catgaagaac tagtaaacaa aaatggagta tatagtaggt tacacaataa
     5041 ctagtataca aattttacac catagctaac atacacatta aacttacaaa cataggatat
     5101 attaagattc ataaagacaa ttcattttat atttataaga tgttttatga ataaaatgta
     5161 tcctatgttt tattttttgc aaattacatt taattctctt tgtaagaata gagttaaata
     5221 gccaatggtc gggataacgt ttttacaaaa agaataattt agaaaactaa ctttaattta
     5281 tggaatttat cgaattttat gaaaaaaatc gaattttgac ttgaaatgtt tttcaaaagt
     5341 agtaaaataa tggataacta tacatacatt ttataaaaat atatttatta tgagggggga
     5401 ttttgtatgg aggcaataac agctttttgt ctagttttag tatcactgac gataggagat
     5461 atagtttcag caaaaactaa agcttttgtt ccgtcggttt ttgtatcagc aattatattt
     5521 atagtaggat tttggacttt tttcccagaa aatattgtgg acttagccgc tttaggaaca
     5581 ccattagctc aattaggtat gcttttgtta ataactcata tgggaactat gatgagtatt
     5641 aaggaattag caggtcaatg gaaaacaata gtaatagcct tggcaggaat agtaggaata
     5701 tgtgtaggtg ctttggcatt agggacagta gtatttggat gggatacagc agtaatagct
     5761 acacctcctc ttactggagg gctagttgca tctataatga tggcagatgc tgctacagca
     5821 aaaggtcttg caagtttatc agtacttgca attttgatgt atgttgcaca aggatttgcg
     5881 ggttatccta ttacagcatt aatgcttaaa aaagaaggaa aaagactttt atcagacttt
     5941 agaagtggaa aagtaactgt aaatacagat gaagaaaaag ttaaggattt acctgaacaa
     6001 aaaagcagat ttaagataat accagactta ccagaaaaat atgatacgac atatatgata
     6061 atacttagat taggatttgt agcatggtta gcagtacaat ttacaaatct tacaaatgaa
     6121 gttgtatcaa gatatgttat atgtttaata tttggtgtaa tagcatcaga acttggaata
     6181 gttgatagaa aaccacttaa taaatctggt tcatttggat ggttaatgac atcattgatg
     6241 gcttttatat ttgcagggtt agcaaaagct acaccagcaa tgcttgttga aatggcagtt
     6301 cctcttgtag gaatcataat atttggtgta ataggtatgg gaatattctc tattatagta
     6361 ggtaagcttt taggtgaaag taaagaaatg gctttttcag tttcgcttac atcactttat
     6421 ggttttccac caaactatat attaactgaa gaggcagtaa aggctttggc tgaaacacca
     6481 gaagaaaaag aatttttaat ggataagatg cttccaaaga tgcttgttgg aggatttact
     6541 acagttacaa tagcttctgt agtaatagcg ggtatctttg taaatctaat atagtattta
     6601 agtgataaaa agtattttaa ataagaagaa tgtgtactac ttggaggtat ggaatggata
     6661 ttaaagaaat aacaaagagt tataaagact atgtaattaa gcttagaagg gaatttcatg
     6721 aaaatcctga gaaaagtatg gaagaagtta gaacatcaaa gagagttaaa gaagaacttg
     6781 ataaaatagg gataccatat gtatcagctg gaggtacagg tgtaattgca actataaaag
     6841 gagctaatcc aggaaagact gtagcactta ggggtgatat ggatgctcta caagttgttg
     6901 aatgcactga tgtagaatac aaatctaaaa atgaaggact tatgcatgct tgtggtcatg
     6961 atggtcacac atcaatgctt ctaggggcag caaaagtgtt aaatgatatt aaggatagta
     7021 tcaatggaac tgtaaaatta ttttttcagc caggagaaga agttggtaaa ggtgcaagag
     7081 ctatgattca agatggtgct atggaaggtg tagatagtgt gtttggaata catctttgga
     7141 ctgatgtaga atctggtacc atatcggtag aagaaggacc aagaatggct tctgctgatt
     7201 tctttaaaat tactgtaaaa ggtagaggtg gtcatggttc acttcctcat caaggtgttg
     7261 atgcagtgct tgcaagttca gcaatagtta tgaatctaca atctatggtt agtagagaag
     7321 taagtcctct ggaaccatta gttgtgagtg ttggagtttt aaattctgga actagattta
     7381 atgtaattgc gagtgaagct atcttagaag gtacaataag gctatttaat ccagagctta
     7441 gaaagcaaat tcctggaatt ttagaacgta tagcaaaatc tacagcagaa gcatatagag
     7501 cagatgctga attagagtat gggtacttaa caccagcagt tataaatgac aaagaatgtt
     7561 caaagatagc tacagatgca gcaattaagc tatttggaga agactgtata acactatttg
     7621 aaaaagtaac aggagcagaa gatttagctg agtttatgaa tatagcacct ggtgcactag
     7681 catttgtagg agctagaaat gagtcaaaag gtgcttgtta tcctcatcat catggatgct
     7741 ttaatataga tgaagatgct ctagaaattg gaactgcttt atatgtgcaa tatgcagtgg
     7801 atttcttaaa taaataattt taatatacta taaagaaaaa gcagagacta agtttatatc
     7861 tctgcttttt tattgtgtat attttttata aatattgact atataaaata tatatgatag
     7921 cataaagaaa atgataatca atatcaatta aaaagaaggt gatttatttg aataatgtaa
     7981 tatttgcatt tttgataaca ttattggctg gtttatctac tggtatagga agttgtatag
     8041 cattttttgc taaaaaaaca aatactaagt ttctttctat aagcttagga ttttctgctg
     8101 gtgttatgat ttatgtttct atgatagaaa tttttcctaa agcacaagat gctttgacaa
     8161 aatctatggg agagaaatta ggaagttggt caactgtaat agcttttttt ataggtatgg
     8221 ctatcattgc tgttattgat aagctaattc cacaagaaga aaatccacat gaaataaaga
     8281 aaatggagaa tatagaagaa aagaatatca aaaaaaataa gtctcttttg agaacaggta
     8341 tatttacagc aatggctata gcaattcaca attttcctga aggattagct acatttatat
     8401 cagctcttga tgatgttaca atagctattc cgatagcaat tgcaattgca atacacaata
     8461 taccagaagg tatatctgta tctgtaccag tttattatgc cacaggagat aaaaagaaag
     8521 ctttttatta ttccttttta tctggtatgt ctgagccttt aggagcgatt attggatatg
     8581 tacttcttag aaatttttta aatgatataa ctcttggaat tgtatttgca attgtaggag
     8641 gaattatggt attcatatca ctagatgaat tattgccatc agctagagag tacggggaac
     8701 atcatttatc tatttatgga ttgattgcag gaatgggtgt tatggctatt agtttattac
     8761 tatttaaata gcttttaaat atatgattgt aacctaaaat tagtatcaaa atgatataat
     8821 tatttaaata gatttttaag ctatttaaaa atatcatttt ggagaatgag tgctatggaa
     8881 aagaaaataa aattcaaatt tgactggtat agttcacctg ataagataga gaaatggtta
     8941 gaagatattg aagctaaagg atataattta tataagataa ataaatttgg aggaatattt
     9001 tattttcata agggaagtcc aagaaaaata aaatacactg ttgattacta ctatgatgta
     9061 gagaataatt attttgatga acataaatca aatggctgga atcttgtatt taggagtatt
     9121 ggtagtttta tgggtacatc tgcatggtat atatggtcga aagaatacaa agacatacct
     9181 cctaaattat atagtgattc tgataaaaaa aacaaagtaa agtcaatttt atttatgaat
     9241 atgtttatat tctttatgtt agtagtaata tttatatatt tattgaatat tgaaataaga
     9301 aatttatata gtggttatgg agcatctgta actgctattg tactacagat tatattgttg
     9361 attttatatg cagtacaatt atataggaga ttaatgtatt atttaaggct taaaaagttt
     9421 aaataaataa aagaaggtgt cctaaaatta tttttttaat attttaagac atcttctttt
     9481 gttaataaag tactgttttt aattatagta atttctgtct ttaaattgaa atatcaaata
     9541 atagttgtat gggattgagt attgataaaa ataatttaaa aatgttatcc taaag
//
LOCUS       DAFKMX010000152         9501 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFKMX010000152 DAFKMX010000000
VERSION     DAFKMX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.152"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..692)
                     /locus_tag="LZB08_002898"
     CDS             complement(<1..692)
                     /locus_tag="LZB08_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBM7316386.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPE"
     gene            complement(732..1079)
                     /locus_tag="LZB08_002899"
     CDS             complement(732..1079)
                     /locus_tag="LZB08_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316387.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1297..2253)
                     /locus_tag="LZB08_002900"
     CDS             complement(1297..2253)
                     /locus_tag="LZB08_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBM7316388.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPIRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2696..3064
                     /locus_tag="LZB08_002901"
     CDS             2696..3064
                     /locus_tag="LZB08_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBM7316389.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            3489..3734
                     /locus_tag="LZB08_002902"
     CDS             3489..3734
                     /locus_tag="LZB08_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316390.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            3833..4096
                     /locus_tag="LZB08_002903"
     CDS             3833..4096
                     /locus_tag="LZB08_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBM7316391.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(4381..5328)
                     /locus_tag="LZB08_002904"
     CDS             complement(4381..5328)
                     /locus_tag="LZB08_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBM7316392.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5331..6962)
                     /locus_tag="LZB08_002905"
     CDS             complement(5331..6962)
                     /locus_tag="LZB08_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBM7316393.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            7092..8009
                     /locus_tag="LZB08_002906"
     CDS             7092..8009
                     /locus_tag="LZB08_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBM7316394.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIEVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(8066..8647)
                     /locus_tag="LZB08_002907"
     CDS             complement(8066..8647)
                     /locus_tag="LZB08_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7316395.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(8687..9253)
                     /locus_tag="LZB08_002908"
     CDS             complement(8687..9253)
                     /locus_tag="LZB08_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7316396.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKLVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
BASE COUNT         3331 a         1419 c         1260 g         3491 t
ORIGIN      
        1 tcttctggag ttctatcaaa tttgagttca aaattttcat cttcaaagta gggaatataa
       61 tataaaggtg ttttgagagc aggtaattta ttatataatt cttcaagaat atcgatattt
      121 ttaggggagt taaagaaaaa gcatttacat acattcatta aatccacagc atctgaaagt
      181 gagttatgtt taatatctcc ttctacagaa aaaatttttt tcatattcag taaagatatt
      241 tgcttagata agattctatt attgtatgta atatgttgag atataaaagg ttgaatgtca
      301 atcatcatat taaataactc taagcattta cctgtatatt tatatactct aaatgaagtt
      361 agaaggccag ttatatcaaa atcaccccaa ttatatattg taacatcatc aaatttacaa
      421 aaccatttat aaaaatcatc cataacatat aaaagattag gagcattatt gatatctagt
      481 tggttcaatt tggttaaatt tttacattta tttgaaagta ttttattgta ttttggctta
      541 actaaagagt aataattatc tattgtatta aattcgttat caactaaaac tccaccaata
      601 gaaattaatt ccgcaccatt atattctttt ggtttatttt tattatcatc tagtatatta
      661 aattcaaagt ctataaataa cttgtatttc ataaatctaa agcacatcct caggtaaatt
      721 atttattgag cctaagcatc ataagaatca atagatataa tgactctaaa catcaaaggt
      781 tcattacaac ttttatttat ttcataattt aattcaaata aatcttttaa gatataattt
      841 ataagtattc tttcccaacc catttttaaa ttaactttag agtctattaa tatattttct
      901 atctttttaa atatttctat atttgctggt attatactga gctcaatatt acttttaaac
      961 gggttagact gtaaatctgt tataagtata ttttttaatt cctctgtaag ttcagaaatt
     1021 aaactatcac ctatgtattt ttttatgatt tcctctctaa tttcttttaa atttagcaat
     1081 attagtacct gctacttaca tagtagcttt tcctttcctt ttaatactat tattcatttt
     1141 tgaatcttat ttagatatat tttaagatta aaagtcttta gctatataat tttttaccca
     1201 tttttaactt atattaacat attacaggct tatatctcca ccctgcttac attgaggatg
     1261 gagtatattg cctattttga taaaaatgat aggttgttat ttgcctattc ctttttctga
     1321 taatttttct aacatatctt tggtcatttt atctaaatca taactaaact taaagttcca
     1381 ttcttttact gcactactac tgtctagaga gttaggccaa gaatcagcta tactttgtct
     1441 tataggatct acatcatatt ctatcacaaa atcaggaata tattttttta tagaatcagc
     1501 tatttcttca ggagaaaagc tcatagcagt aatattaaaa gcatttctat ggatgagttt
     1561 atcagctgga gcttcgatta aatccactat tgattgaagt gcatcaggca tatacatcat
     1621 atccattttt gtaccttctg ctatgtaaga tttatatctc ttatttttta atgcctcata
     1681 atatatatca actgcataat ctgtagttcc tccaccagga ggcgtaacat aagaaataag
     1741 acctggaaat ctaacacctc tagtgtctac tccaaatttt gagtgataat aatcacatag
     1801 taattctcca gaaactttag tcacaccata catagtatta ggtctttgta atgtatcttg
     1861 aggagtcata ttttttggag aattttcacc aaaagcagca attgaactag gtgtaaaaaa
     1921 tttacaatcc aattctttgg ctatttcaag accatttatt aatccattca tatttaagtt
     1981 ccaagcttct agaggtttac tttcagcaac tgcagaaagt aaagatgcta gatgaattat
     2041 agtatctaca tcatattttt ttgcaatttc aaaaaaacga ttcttatctg taacatctaa
     2101 tatctcaaat ataccagatt cacatactgg attaccttct tttactctcc ttgaactagc
     2161 gattacattt tgttctccat actcatttct aagctttatt gttaattctg aacctatttg
     2221 tcctaatgca cctgttataa gtattttttt caattttaca acctcctaag ggtttaattt
     2281 atgtagtact acttattttt attttagcaa atcttttgtt taaatacaaa gaattaaaga
     2341 taataatata cgacttaatt gaatgaagtg cagtaagtgc ctaaaaattt aaagctaata
     2401 tataatggta tattgtataa caataaaaga gtagattaaa ttattatata tagattgtgt
     2461 gactaagtca cagaaaatag agttaattta atatataata aaagcatatc aagtaaagct
     2521 ttaaattaaa gtaactttat ataaaaagta atataaattg ataaaattat aaatttattt
     2581 atagtgaata tggggtggat tttatagata caaaaatagt atatatgata tgtagaccat
     2641 tagcatgttt aaagtagcaa aacatataaa ataaaatttg aaaggaagta taaaaatgac
     2701 aagaccatca atatatcata gccaaggata cacttgtgca gaggcattaa taaagtctta
     2761 taatgaggaa cacaacacag atataccaat ctcaatagga agtggtatgg gggttggaat
     2821 gaatgttgga agtgtttgtg gagctgtaaa tgctgctgtt gttataatag ggtatttaaa
     2881 tgggcgaaat agtaatttag atgaaaatat agcaaggaag tattcaagag agctattgaa
     2941 tagagtaaga aataaataca gctcagaaat atgtgtatct cttaaaaaaa ataaagtaac
     3001 ttgtgaagaa ataataaatt tcacatatga tgctctaaat gatatgctaa aaaatcaaaa
     3061 ataattctaa gaaacaagta ttttacattc gatagaatac ttgttttttt atgatatttt
     3121 taataggtat tagtttatta taatttttaa atcaatttta ttatatttta attgaaataa
     3181 tctactttga aaattactag ctgacaaaag aatatgatta tagtatagaa aaaatgtaaa
     3241 ttatttaata ttaagtgaga gtatatgctt acctatgtaa aatcgtataa attaatggct
     3301 aaatttataa attataaaga aatatatagg aaaacaaaag aattaattag tataatataa
     3361 agttaaaaag ttaaaattta tataatttat ttataaagta aagaaaaaat agaaatttgt
     3421 aataaaataa aaagtttatg acataatatg gtataattag taaaaatata gagattggag
     3481 gaattgcttt gtttatagat gaagaattag aaggttatat attaacatgt aaaatatctg
     3541 aagactttaa aaatatacct gaatatagtg atgaagagtt ttatgttaca gtctataaag
     3601 atgaaagttc tgactctggg tactatgctt tattagaaaa taaagaagaa agagttgtat
     3661 gggatggaga agttgttgcc aataatattt ttaataacct ttggattgta gtaaataagg
     3721 ttaaaactgg ataatagtct aattatatag tatttgtaat attttaaatg aatttttaca
     3781 tatttgagat gagtaaataa caaagattat tcgatactgg aggagtaatt caatggaata
     3841 tagttatagt aagatgaact taaaaaaagg ggatattgta gaggtaaatt tagagaaaca
     3901 agccaatgta atattgttag accatataaa ttatgtgaaa tttaaaaatc aaagaaatta
     3961 tgattactat ggtggatttg caaagaaaaa tccatgtaga atgagagttc ctaatactgg
     4021 aacatggtat ctagtagtaa atcaagatgg aaatagtgga atagtaaact tttcaatcaa
     4081 tacaattcaa aattaatttt agaatattgg atattcttac tttttgtact ttttatatag
     4141 ttaaaataaa attaataagc aattattata aaataatgtg ggtgcctcaa aatgaatttt
     4201 ataattcacg agacactctt ttttgtataa acttaaatat actttatcat ttcaacaatg
     4261 aaaaagaggc gtatctgtgt tttgagatag cctcatttat catatatcat atatagaatt
     4321 ttaattattc ttaactaaaa ttttttccat aggttatctg tacaattaaa atatttattg
     4381 ttagcataca gctccacctg taaggtgtaa aagagcctct agtatctctt cttttctttc
     4441 caaaacttca tctgataaaa gcatttctat aaatttatca gcaccgattc tatcgatagt
     4501 tattccaaaa cgttcacctg tgttaccctg ttctctaaat aaaagaattg ctttttcaat
     4561 taaagtcata agttcctctt ttgaaaataa tctatcaatc tgtgttccag gacgaactga
     4621 ttttccccat ttccctccta tatagacttt ataaccagat tctttttctt caatactatc
     4681 aaaattacaa ctttctatac acttaccaca gttattacat agattactat ctatttcaag
     4741 tttaccttta tctgttaatt tagctgcttt aacaggacag acctctacaa cagcacattt
     4801 tttacaacca acacaaagct ctgaatcata gtcaggtaca cgttgaccaa caattccaag
     4861 atcgtttagg tcaggtttaa tacagttatt agggcatcca ccaacaccaa ttttgaactt
     4921 atgaggtagt tttacatcat accaattttt gtaaaattct tcgtgtattt cagcagcaag
     4981 tgcctgtgta tcagctagac catgaacaca aacagtacct ttacaagcca caacaggacg
     5041 aacccttgat ccagttccac ctgttattag attttcgcta gcaataaatt cacttagagc
     5101 ttcaatatct tcaaatttta caccaggtaa ttctactgtt agtctagtag taaatgctaa
     5161 ctgaccgttt ccgaattttt cagcagcttc tgcaattttt tttgcttgtg atgcatttat
     5221 tacaccattt acagttatta tacgtgctgc aaaatgttct ccatccctgc ttggtagaaa
     5281 acctctacct tttacttcct ttttttgttc tgctgtaaca ttactcatat ttactcctcc
     5341 attaccttaa gatttggata aaatagtatt ccaccttcaa gaactttcgt atttgtatag
     5401 ccaaagcgtc ttaagcgatt ttgcgccata tagctatttt tacctttagc acatataaga
     5461 agaatttttt catccttagc aaggccttca atttctccat taatttcacc aacaggaata
     5521 taacgtaaac taggaataga aggagctttt gaaacatcaa gtttagtcca actgtcaaaa
     5581 tcctcaattt cgtcaagtaa aacactatca agttctccat taagtttatt ttgtaaaaca
     5641 tttactgcca ctacaaaagg gtgaatagct gttgaaaaag gtggtgcata tgcaaggtcc
     5701 atactttcta atgaataaag gtcagcgcct aaagttattg cagtagcagc gatatctact
     5761 attttatcaa ccgctccagg tcccataact tgaacaccta ataattttct agtatttttt
     5821 tcagcaacca ttcttataat aaaattagat gcacctggat aataatgagc tttatcatca
     5881 gtagctatgg ttacagagca aacattataa ccttcttttt cagctatttc tttacttaga
     5941 cctgtttttc ctgcattgag ctcaggtaat tttactactg ttgttccaag aacaccattg
     6001 taagttttag attttccaga tatattttgt gcacaaattc taccctcatg atttgcagat
     6061 gaccccatag gtgaccatgt aggtttattt gttaaagaat tttttacaaa agcacagtct
     6121 ccaactacgt atatatcttt gtcatttgta agcatatatt catctgcaat aattgtatta
     6181 ttaggggcaa attctaaacc tgtgtctttt agaaaacctg tgttaggacg gattccaact
     6241 gacataacca ctaaatctgt tttaatagct ctatttttag tacgaagttt ttcaacttta
     6301 ttttcacctt caatacctac aacctggtca cctgtaaaaa tcattatgcc tttatcagcc
     6361 atatgatttt caacgtattc agcaaaatca gtatcaaatc caggcataac atgttcagcc
     6421 atatcaacaa cacttacacg tatacccatt gcagaaagat tttcagcaat ttcaagacca
     6481 atgaaacctc caccaataac aacagctctc ttaataccgt tttcgataga atctcttagt
     6541 tttatagcat catttggagt acgcataaag aatactccag gtaaatctat tccaggtatt
     6601 ggtggtttta ttgggtctgc ccctgtagca attacaagtt tgtcatagct tagattttct
     6661 ttttctcctg ttgaaagcgt tttcaaagtt acttgttttg catttctatc aatagatgta
     6721 acttcaactc cacaacgaac ttctgctcct gttagtttag aaaaactttc aggtgtattt
     6781 acgattaaag atgatttatc ttcaattatc ttaccaacgt aatatggaag accacaacct
     6841 gcataagaaa tatcatcacc tttatttagt attgtaacag agcaattatc acccatttca
     6901 cgttttattt ttgcagcagt ttttgttcca gcagcaactc cacctataat taatactttc
     6961 acaatatatc acactccaat ctactttata actctataat aaaattatac ttcttgacaa
     7021 tatatttgta aaatactaaa atattaataa aatcataagc atttactaat gtttgctaag
     7081 gttgtgataa tatgacaatt cgtagtcttg aaatctttgt taaggttgct gaatgtggta
     7141 aaatgagtga agtagcaaga aatatgtata ttacccagtc atcagttagc caagctattt
     7201 cagaaataga aaaagagtat ggtgttaagc tatttgatag aatttcaaaa aaattatatt
     7261 taactgaggc agggaaaaaa cttcttggat atgggaggca tcttttagct gtaaatgaag
     7321 aaatgaatga ttgtatgaag cattgtgcaa aaaatatccg tataagagtt ggggcaacag
     7381 taactgttgg tacatgtgta ataagcccga taatgttaga attatacaag gtaaatcctc
     7441 ttatagaacc tgaagtattt gtagaagata caagactgat tgcaaaaaaa ttacttaata
     7501 gtgaattaga tattgcgatt gtagaaggaa aaattaagca cccagatatt gttacaaaaa
     7561 gcattattaa tgataatctt gtacttatat gttcacacaa gcatgagttt tataaaagag
     7621 actctataga agtttcagaa ctttcaaatc aaccattaat tatgcgtgaa ttgggaagtg
     7681 gtacaagagc acaattagaa aagcaattaa aagaattaaa aatcccaatg aatattagat
     7741 ggtcatgtta taactctgaa gctatattaa gagcagttgt agataatttt ggcattgcag
     7801 ttatatctga attacttatt gaggattatt taaaaaagca tcttttgtgg gcttgtgata
     7861 ttgaaggtat taatttacat aggacatttg atattgcata tcatagaagc aagtttttta
     7921 cagaaaatat ttcagcattt tttgatattt cagtagaata tgggaaaaaa caagcgagaa
     7981 agaagagtag aagtgtaaac agtttataat atatctaaat aaaaataaga gtatatcaat
     8041 atactcttat ttttatttag atatattagc ctatattaac gccaaaattt ttacacaatc
     8101 ctgcaagtcc atcctcaaat ccagaaccta aagcattaaa tttccactct ccattatgtc
     8161 tatatagttc tgccacaact attgctgttt ctatgctaaa atcttcaccc aattcatatt
     8221 taataagttc ctcattagtt tcttcattat atattcttat atatgaattt tcaacttgtc
     8281 cgaaattttg tcttcttgta attgcttcat ttatagtaac actaaatgat attttagcaa
     8341 tattttgagg tattttagat aagtctacat ttatttgttc atcatcacca tcaccaacac
     8401 ctgttctatt atcacccata tattcaacag ctccagaagc gtgttttaaa ttattataga
     8461 atataaagtc tccatcattc gttacttttc catcagttcc agttaaaaat gcagaggcat
     8521 ctaaatcgaa atcaaagcca ccatcatatt tattaatatc ccatcctagt cctactatta
     8581 ctttttttag accagggtta ctttttgtta aacttacttt ttgtcctttt gctaaagtta
     8641 tacccattta aaccaactcc tcattataaa ttttttattg taattattac ttatatgtat
     8701 taattaattc acgaagctct tcattttgac caattcctaa agcattaaat ttccattctt
     8761 catttctacg ataaatttct cctgccaaaa ctgttacttt accagaaaag tcttcttgaa
     8821 gattatattt aaataattct tgcttggaat ttgaatcaat taatcttata aaagagttgt
     8881 tcatttgacc aaaatcctgg tctctttttt tagctttgta tatagaaact ccaaaaacta
     8941 atttatgtac atattcaggt atgtcttcta aattaacaat tatttgttca tcatcacctt
     9001 caccttttcc agttaagtta tctccagtgt gtataatagc tttagaaggg tgtttaagat
     9061 ttccaaaata aaccaagtca cctctaacat tcattttatc ttggttagtc aatgctattg
     9121 caaatgcatc taggtcataa ttttttttac ccaatttatt catatcccat cctaagccta
     9181 ctactagatt ttttacagga ttattatctt ttactaagct aactctttgt cctttttgta
     9241 aactaacact cattttaagt tctccttttt atatttttag ttttattgat ggtatcaagt
     9301 ataagaacca tattagctat attatatacg tatttaggct aagttggcta tatttataaa
     9361 ttatttatta tataactaca tgaatatttt ataatttaaa catattgaca ttatatgctt
     9421 taaattattt tgtagtttta taatatttaa atttgtattt gattgttata aagtaaagta
     9481 agtaataaag ttagactcaa t
//
LOCUS       DAFKMX010000153         9444 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFKMX010000153 DAFKMX010000000
VERSION     DAFKMX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.153"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(100..708)
                     /locus_tag="LZB08_002909"
     CDS             complement(100..708)
                     /locus_tag="LZB08_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBM7316397.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(817..3555)
                     /locus_tag="LZB08_002910"
     CDS             complement(817..3555)
                     /locus_tag="LZB08_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBM7316398.1"
                     /translation="MESKGTLSKNKVYILANGLFLAIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3703..4494)
                     /locus_tag="LZB08_002911"
     CDS             complement(3703..4494)
                     /locus_tag="LZB08_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7316399.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4530..5186)
                     /locus_tag="LZB08_002912"
     CDS             complement(4530..5186)
                     /locus_tag="LZB08_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316400.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5179..6144)
                     /locus_tag="LZB08_002913"
     CDS             complement(5179..6144)
                     /locus_tag="LZB08_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBM7316401.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6250..6356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6646..7590)
                     /locus_tag="LZB08_002914"
     CDS             complement(6646..7590)
                     /locus_tag="LZB08_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBM7316402.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7701..8168
                     /locus_tag="LZB08_002915"
     CDS             7701..8168
                     /locus_tag="LZB08_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316403.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8338..8937)
                     /locus_tag="LZB08_002916"
     CDS             complement(8338..8937)
                     /locus_tag="LZB08_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316404.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9183..>9444
                     /locus_tag="LZB08_002917"
     CDS             9183..>9444
                     /locus_tag="LZB08_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 domain-containing protein"
                     /protein_id="HBM7316405.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDLIVIDKEKKGLKILFVNKKAL"
BASE COUNT         3103 a         1513 c          925 g         3903 t
ORIGIN      
        1 tataaagtta tttttatcaa actaaaaatt ctataaaaaa ataagataca tacctataaa
       61 ataccttcct atagatacat atcttaccta tataaaaact tattctatat aaaaacgcca
      121 taaaaaatct tttgcttctt ctgcataatc tatatttata cgtttactag tttttatatc
      181 taactcatct ttactaaata ctttttttcc tttatcatca taataaaaat cacttatata
      241 taattcttca cccattatac tcttactatt tagacttcta tctattttta aagctttgca
      301 aagttttcct ggtccgttag taatattttt tacttgatat ttactcaact cagtgtaagc
      361 tttgttgtat ctattcatag atatctcatc taaagaagtt ataggctcta cccctcttat
      421 taatacacac tctggcacat tttctatatt tgcagataaa tttaagcaat tatacatacc
      481 ataaattaaa taaacatata tatgtccacc atctaaatat aatggcttag ttctatctgt
      541 ctttttattg ccatatacat gtgctccttt atcatttaca cccatgtatg cttctgtctc
      601 tatgattttg gtaacaatta ctttattttc atatttcctt attagatatt tacctaatat
      661 tgatttggct aaatcaattc cattttttct aaaaaaatcc ttttccaaaa tttcacctca
      721 taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat aaataaaatt aactcttatt
      781 aatgtgatat atataaacaa taaatttaga catttattat ttatatatat caaaaaattc
      841 tatctcaaac ttttcaatat tatcattgta atttgaatta tcatatactt cttcaacaac
      901 ctctttaatt gggaatataa cttttacatt agttccttct cctaccttac tatctatgta
      961 aatcttccca ttttgaattt caatcaatga ttttgtaagg cataaaccta taccacttcc
     1021 aaattcattt gatataccac tgttttcaat ttgtttaaat cgattaaata tatcatttaa
     1081 tttttctttg tctatgccca ctccagtatc ttttataatt atctctactg tttcgtcttt
     1141 tttatatata cctacaagga tttctcctcc ttctaaagta aattttatag catttgataa
     1201 tatatttagt attattcttt ctatcgattc tgggtcaagc cctaccataa cagtttcttc
     1261 atctgtatca aaaattaaat ctattccttt agacttagca taactactaa tagatgtaac
     1321 tattctttct gttatgtcaa taatatcaaa attaactgct ttaatcttat atatgtctga
     1381 cttaacttga gaaatattta ttatattatc tattaatctc aataatctaa acatattttg
     1441 tttagttatt tttatatgtt cttttagtat atcctgaaaa tcaattattt ttcctttttt
     1501 ataaaggttt tcaataagtt gaactgaact atatatatta tttaaaggtg tcttaagttc
     1561 atgagatata ttagcataaa actccatctt catttggtca tactgtcttt gttttattaa
     1621 tgctttttta agtctttcag actctctttg cacacttaaa tctttaataa ttctcactct
     1681 acttcttttc tgtccaaagt ataatgttct tgatatcatt tttacttctt taatgtgatt
     1741 attttcattt actatatgga tactcttctc tctttctcct atatttaaat tgtttatgta
     1801 tttttttact aatatatttc tatatttttg tgttgtaact cctaatacat cactagaaat
     1861 tccgaacaac tctctaaagg ctctatttgt atatagtact cttgatgtac ttaaatcctc
     1921 aagaaaaata gcctctggta taacatccag taatttttca tatattattt tttgttcatt
     1981 tactgttcta agaatattta tctctttagt tatatctcta aatgtaagca atttaaagag
     2041 tttattatct gaccaatagt ctccaactct aacttcttca acatgttctt caccttctga
     2101 gtcagacatc acaaaattaa ctatacagtt tgctgtgtca ttgtaactta ctatttcagc
     2161 tttcttttct tgtgttagat ttaatctttt aaattcccta ttgtcaaaga aatctatgtt
     2221 aaaagtttca aaatattgtt ctaatgtcat atcgactaaa tcacttgaaa cacatttaaa
     2281 actcttttca tattttttat ttatacttat taccttattc ttctttatgc caccttcagc
     2341 acttcttaca agtataggag atggaatatt tagtataatg ttttcatagt ctttatttat
     2401 atttcttatt tcttcatcta ttttgatttc atttgtaata tcatttaaac ttattaagta
     2461 attgctttcg tctttattat tacttaaact taacaaataa attttatcat ttaactctac
     2521 cagtttatca attatactta tatcgttatc aaatttcttc aatttttcag caactttttc
     2581 atcttccttt ataaaagtta atattttata ataattattc atatcctttg aaaattcatc
     2641 tttaaattta ttatttgtgt aacttatatt aaaatcatag tctgttacta tccatacatt
     2701 tggtattgta tccagagctt gtctaagtaa ttcttttctc tggtctatgt agttttttaa
     2761 tgttctttgt ataatcatat ccttttctat tgctaaattt atctttccta attgctctga
     2821 ctttttactt aattttttat tgattgaatc aagttcataa tatggataca ctactctatt
     2881 attaagactg tatacatata cataataagt atggttacat aatagtaaat atattactcc
     2941 tattaatcta tctgaatcta ctatattaat cttagaaatt gctacaaaac atataatagt
     3001 tgtaaaaaaa acaaaatatg ttaaatcttt tattaactta tagtgctcaa tgttactatc
     3061 aagtttattc aacaaatata gtaaattaaa catagatata caaatattaa ttaatataat
     3121 aatcatattc cagttaatat cactaaaaat atttcttata aagtaatcta atatcacaaa
     3181 agcactataa aaaattagta ttgggcttat tcttagtttt tttactttag aaatggttaa
     3241 gtatataaaa gtagacatat taattatgct taagttaagc aatacaattt ggaatttttg
     3301 aatttttaaa tttactactc ctaaaatact tacaaaaact aatacaagac accctacttt
     3361 caatatattt ataaattcat ctgttttaaa gctacttata taaactatag aatatccaaa
     3421 tatccaaagt aaaattattc tatacatatt tatagaaact gtatacaaat ttatatttaa
     3481 aagtgcaata gccaaaaata acccattagc tagtatatag accttattct tacttaaagt
     3541 tcctttagat tccatattcc ccaccacttc ttcattttta tatagtttta cctataaata
     3601 tgttaccaca attcttccaa aaaatttgtc actttctagt tataaaaaaa aatgagtaaa
     3661 tatttttaat tttactcatt ttgtagattt tgtatattca gtttaaaaag ctggaactat
     3721 acttccttta tattcatctt gtataaattt tttaacttct ttactattca tagcctctga
     3781 taaagcctta atcttatcac tatctttatt attttctcta caagctatta tatttacata
     3841 tggagaatca cttgattcta ttactattgc atccttagta ggatttaagt tagctgttaa
     3901 agcataatta gaatttatta ctgctccgtc aacatctttt aatactgttg gtaattgctc
     3961 tgcattcatt tcttttatta ctatattttt aggattttta gttatatctt ttttagttat
     4021 aagctctcca tcttttactt ctatcaattt attttttgct aataatttaa gtgctctagc
     4081 tccattagtt gcatcatttg gcacagctat aacagctcca tcttttaatt catctaatga
     4141 cttaactttt tctgaataga atcccattgg ctctatatga actttagatg tataagtaag
     4201 tttatatcct ttctccttca cagtttcctc taaaaatgga atatgttgaa agaagttagc
     4261 atccaaagac ccttcatcta aagctgtatt tggaagtaca taatcatcaa atatttttac
     4321 ttctaaatca tatccttttt cttttaacaa aggttttgca acttccaata tctttgcatg
     4381 tggatttgat gaagctccta ctagtatttt tttatcctct ttgtttgaac aacctactgc
     4441 tgatattgct attgctgaca ccaatgctac tgataataat ttctttaatt tcatttttac
     4501 tccccctctt taaaatgttt ataattaatt tattttagtt tcttataagc cagatttcct
     4561 actccctgga ttatctgaac taatattata agtgctatta cagtatatac cattactgct
     4621 gtatcaaatc tttgatatcc ataaataagt gctatattac ctaatcctcc agctcctact
     4681 gcacctgcca tagctgtata accaagtata gatattattg ataatgtaat tccagatact
     4741 attgaaggca tagcttcctt tatcataact ttaaatacta tttgtctttt tgtagctcca
     4801 aaagattttg ctgcttctat caatccttta tcaacttcat ttaatgagct ttctataatt
     4861 ctagcgataa acggtgctgc cccaatagtt attggcacta ttgcagcagt ttcccctata
     4921 cttgttccaa cgattaatct agttattgga atcattgcaa ctataagtat cataaatggg
     4981 aaacttctaa atatatttac tataaatcct aatgcactat ttattgtgct atttggtttt
     5041 aatccatctg gttttgtaac tactaatatg attgctaata taaatcctaa tattgttgcc
     5101 actatagttg gtactataac catatacaaa gtttgtaaca atgcatttgg aaatagtgtt
     5161 gttaaaaagt caattaaact attcatttat catttcctcc catcttatgc ctttactagt
     5221 tagatattgt tttacttgct ctccactttt atctgaaatg tttataataa gtgaacctaa
     5281 aatatcatct ttaaattgtt ctaatttccc aaatattata gacacatcta tatttagttc
     5341 tcttgccata gtcgtaataa tagcttcatt agaaatatct ttagggaata gtatctttat
     5401 atttgttcct tttggcaata ttatactttc ttcacctatt aattttctaa gtcctttagt
     5461 atttcttaag aatatttctt ctgtatctcc tacctctaat acttctccat tttccattat
     5521 tgctactctt ccacaaatct gctttataac ttccatttga tgagttacaa ctattattgt
     5581 tattcccaat tttttattta tatcttcaag caatgaaaga attgactttg tagtatttgg
     5641 gtctaatgct gaagttgctt catcacaaag aagtacttgt ggatttagtg ctaaagctct
     5701 agcaattgca accctttgtt tttgtccgcc acttagattt ctgggtttat catttttctt
     5761 ttcagatatt ccaactactt ctaataactc taaaactctt tttttaattt ctgcctttga
     5821 gtatccaaaa cattctaatg gcaatgctac attatcaaat acagtttttc tttcaagcaa
     5881 tgaaaagtgt tgaaaaatca ttcctaactc ttttcttaaa tctctcattt gcttttcatt
     5941 caatgacttt acttctttat cagaaacaag tacactgcct tcttggtatt cttctaatcc
     6001 gtttatacat ctcagtagtg ttgatttccc tgcaccacta tgacctatta ttccaaaaat
     6061 ttctccagat tctatttcaa tacttacatc tttaagcact tgaatctttc cataatactt
     6121 attaacattt tttatgctaa tcataaaatt tcctcccata tattctgctc agtttttatg
     6181 tttatcctct cttatgtatt acaacaaaat aaaagcccga gtataaactc aggcttagaa
     6241 agctatttat actcatctat caactatatt agttgcagga tttagcacca atgtatataa
     6301 aatatactgg ttgccgggtt tcaaagggcc agtccctcca ccactcttga taagacgtta
     6361 tttaatttaa ttctattatc atttgttgct atgttatata tgatatattc attaatagta
     6421 tttgtcaata aatttattta aattttttat tttttattat cgttaattaa ctatttttaa
     6481 ttagttttct agctcctaac actttatatt ttaatgtcta tataactata gaacatcatt
     6541 ataagaaata aattaaataa catcaatcta tctaaatcat ctgtaaagta aattaagaaa
     6601 attaacattt tatccaaact ctatcctact attaaataaa ataatttaaa tacaatgatg
     6661 attatatagc tctagtactt tagactttat caaatgttta agttctatag gttctattat
     6721 tgtagcattt ttcccaaaag ttaaaaaata ttcagccacc cagttaagtg tagattttgg
     6781 tatatacata tctatgtatc cacctttaga caagattttc aatcctcttg ccaacaaaaa
     6841 ttctgtttca caacatttta ctccctcatc tgtcaaatta attttaatat gataatctgt
     6901 acctacttcc atattcttaa gataatcatg tatagaagat aaaaccttat atttcttact
     6961 tgataagttt tctatactca aaatcttcac aattctgtct acacgaaatt ccttaataga
     7021 ttctgatttt atacagtaag ctggacaata ccaaagtcca ttcatggcat acaaaccaat
     7081 aggaacaatg gtacgtacac tatttttttg ctttgatgaa tattcaattt taattgtaga
     7141 ttcattcatt actatattaa acaattcttt caataatggt gttgaacagt gcctatctgg
     7201 tgtccaaaaa acaaatttac gccttatatt ctcaatatta tattgaatat catttggtat
     7261 acaatttaaa aattttttta aaacagaaat agtttcttgc tcaaatggca aatcattata
     7321 atattctagt gactgatatg caaagaatat agattttacc tcactttctg aaaatgcaat
     7381 cggtggaagt atacgctctt taagtacatg atatccacca gccgctccaa cttttgaata
     7441 taatggaaat ccaatactct ccagcacctg taagtctctt aatatagtac gttttgatac
     7501 agaaaattct tctgctaatt ctcctgctgt aaaacttctt tttgcattta ttgttatcat
     7561 cagttcaatt aaacgctcag ctcttgacat atttatactc ctaatttaaa tatagtgaca
     7621 ataattgtca ctattatatg ataagatatt cttacattta aagcaataat aatagtaaag
     7681 aaagaaggga ttgatatttt atggaaaatg atactattaa ggctgatgat attctcaatt
     7741 attgtctatc aaacttagat gatgttgtac taatggatag ttggggggaa cgagcaattt
     7801 actacaatcc taatggtgtt ttaaagcgag gggtatatgt tcttaccatt aaggaaaaag
     7861 acagtaataa tgataaaggt tcgttagtta gtcgtccaaa tgtataccgt gtgaatatag
     7921 gattaaaaaa agaaactttt attgaaatgt ttggatatat tccaaagcgt ccaggtgtag
     7981 gtcaaatagt tgatatggat tttgatttta caaaattgga cacaatcatg ccacatccta
     8041 tctactcatg gatgggatgg atatgtgtcc taagccctac tgaaaagaca tttgagaact
     8101 ttaaaacttt aataggagaa tcttacaatt ttgcaaaaca aaaatttaaa aagagaaaaa
     8161 acaagtaaaa acgtgtgaaa ataacctcta aataaattat aataaaaata ttaaggttac
     8221 aagataaaaa ttaatatctt aataacatgc aaaaaagcga agccaaaaaa tgtattattt
     8281 ataaggcttc gctatatctc aaataattta tttcaaattt caaaactatt tattctatta
     8341 tttcttatta tttcctgtta ttcctaatct atcattcata tcttttatca tttttctaaa
     8401 accactacct atatcatttc caacatcttc agttccatga actaatttta taaagtcatt
     8461 ttgacctttt ttcatatctt catcagatac cttatctaat ttttctattc tcttatctat
     8521 atcttttact aatctttccc cttcagcgat taggtcatca ttcattttct ttaactctgt
     8581 atcttctgtt ttaccatctt ttttaagaga tgttacaaat gattgtattt tatctctact
     8641 atctttatat gcagccttta catcacttag atatttttca tttcctggat attctttatc
     8701 tttaaactga ttatttatac tttctggagt atcataaata gaatatctat ttagacctgc
     8761 tatataatca ttatagcttg ttgaataata attcatatat ccttcattat tatttacaga
     8821 ttcattagga ttttttcctg tattttcact tggtttttca ttatttttag tacatgcagt
     8881 tatacctata gctaatacta gtgctaatcc tacaaataaa gttcttttta atttcatttt
     8941 tattaccctc ctagatgttt tatactataa aaaattttaa taattttctt tcttaattta
     9001 gtatttctat atttttatat tttatttact aaaattaaac aagttataat ttattttttt
     9061 ggaaatcata atgaataaat aattaaataa aaaaattata attttattat tgatattgat
     9121 tatcattcat agtataatct aattacttga tattaatttt catttgaagg aggttatctt
     9181 ttatgtcttg ttgtaattca caattttgtt gtaaaaataa aattgaatca tcatatataa
     9241 aatggctttt agaagtttta ggtccagtct ttttaggctc taaacctgct gaaataataa
     9301 atatttcgtt tactgatgta aatagagagg aaaaaattaa tgatatttat aaatatctat
     9361 ataaatgtaa gaaaatagat ttaatagtta ttgataaaga aaaaaaggga ttaaaaatct
     9421 tatttgtaaa taaaaaagca ttat
//
LOCUS       DAFKMX010000154         9396 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFKMX010000154 DAFKMX010000000
VERSION     DAFKMX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.154"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1592
                     /gene="aspD"
                     /locus_tag="LZB08_002918"
     CDS             <1..1592
                     /gene="aspD"
                     /locus_tag="LZB08_002918"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBM7316406.1"
                     /translation="GKISPFEFKDKLIDIAKYTAKENNRELLDAGRGNPNWTCSTARE
                     AFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDMPGAYLARDIINFG
                     INELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQEMKYDISGKYGDVE
                     IFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPNLPRYDFKVVNINA
                     NEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDETSCNNLIDVIENYN
                     KDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWRLGTFALHKKNVFD
                     KKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNHTAGLSTPQQVQMA
                     FFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAAYYTQFDIEEWAKL
                     NYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIRISLANLYDEAYTK
                     IGKAIREILNGYIVEWQKIKK"
     gene            1713..2708
                     /locus_tag="LZB08_002919"
     CDS             1713..2708
                     /locus_tag="LZB08_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBM7316407.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2886..3268)
                     /locus_tag="LZB08_002920"
                     /pseudo
     CDS             complement(2886..3268)
                     /locus_tag="LZB08_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3494..3591
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3733..4088
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3733..3761
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4374..4532)
                     /locus_tag="LZB08_002921"
     CDS             complement(4374..4532)
                     /locus_tag="LZB08_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316408.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(4891..6261)
                     /locus_tag="LZB08_002922"
     CDS             complement(4891..6261)
                     /locus_tag="LZB08_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBM7316409.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6545..6643
                     /locus_tag="LZB08_002923"
                     /pseudo
     CDS             6545..6643
                     /locus_tag="LZB08_002923"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6662..7083
                     /locus_tag="LZB08_002924"
                     /pseudo
     CDS             6662..7083
                     /locus_tag="LZB08_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7314..7586
                     /locus_tag="LZB08_002925"
     CDS             7314..7586
                     /locus_tag="LZB08_002925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7316410.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(7949..8029)
                     /locus_tag="LZB08_002926"
                     /pseudo
     CDS             complement(7949..8029)
                     /locus_tag="LZB08_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8069..8737)
                     /locus_tag="LZB08_002927"
     CDS             complement(8069..8737)
                     /locus_tag="LZB08_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBM7316411.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
BASE COUNT         3358 a         1420 c         1277 g         3341 t
ORIGIN      
        1 atggaaaaat aagccctttt gaatttaagg acaaattaat agatattgca aaatacactg
       61 caaaagaaaa taacagagaa ttacttgatg caggtcgtgg aaatccaaac tggacttgtt
      121 caactgctag agaagccttt tttacttttg gtcatttcgc tattactgaa actcgttcta
      181 attgggattt aggtcatttg gctggaatgc ctcaaaaaaa gggtattaaa gaaagatttt
      241 ttaaatttat aaatgaaaat atagatatgc ctggagcata tttagctaga gatattataa
      301 actttggtat aaatgaactt ggatttgatg gagatgaatt tgttcatgaa ctagctgatg
      361 gaattattgg tgataactat ccacttccag atagaatgtt acctcatatg gaaaaaatag
      421 tacacgatta cctagttcaa gaaatgaaat atgatatatc aggtaaatat ggtgatgtag
      481 aaatttttgc tgttgaaggt gctacagctg ctatgtgcta tatatttgat tcattaatgg
      541 caaatgaact tctaaaaaaa ggagatacaa tagccttaat gactccaata tttacacctt
      601 atcttgaaat acctaacctt cctcgttacg actttaaagt ggtaaatata aatgctaatg
      661 aagttgatga aaaaggtgct cacacatggc aatatacaaa aaaagaatta gagaaacttc
      721 gtgacaaatc tattaaagct ttattcgttg ttaatcctaa taatcctgct tctatagcta
      781 tggatgaaac atcttgtaat aatctaatag atgtaattga aaattataat aaagatttaa
      841 tgataatatc agatgatgta tatggaactt ttgtagaaga tttttcatcc ttaatgtcaa
      901 aactacctta caacactgtt ggagtatatt cttattcaaa atattttgga gttacaggat
      961 ggagacttgg tacatttgcc cttcataaga aaaatgtttt tgataagaaa ataaatgatt
     1021 taactggaga attaaaaaaa tcagtagata agcgttacag tgatatgagt cttaaccctt
     1081 ctagcctttc atttatggaa agagttgttg ctgatagcag acttgttgct ctcaaccata
     1141 ctgctggtct ttctactcca cagcaagtac aaatggcgtt tttctctgct tttgcattga
     1201 ttgacaaagt agatgcctac aaaaaactca atatgagtat ctgccataga cgtcaaaaac
     1261 tattatttga agctttagag cttccaatta atgaaaacaa aaacaatgct gcatattaca
     1321 ctcaatttga tattgaagaa tgggctaaat taaactacgg agaagactta tttaaattta
     1381 taagtaaaac tgcatctcca gtagatgttc tttataaatt agctaatgac tattcagtag
     1441 ttttacttag tggaaatgga ttctatggtc ctgaatggtc aattagaata tctctagcaa
     1501 atctatatga tgaagcttat actaagatag gaaaagccat aagagagata ttaaatggtt
     1561 atatcgttga atggcaaaaa attaagaaat aattatatta cactgaaata tctaaattat
     1621 atgaataaaa gagtttatgt agaccttaac tacttttttt gatataatcc tatcggaatt
     1681 ataaatttta tttatgaaag gcagagaata gtatgagtat taagaaaaaa gttgccatag
     1741 tttttactgg tggaactata tctatgacag tagatgataa agtcggagca gctataccaa
     1801 ctttatcagg tgaacaaata ctatcaatgg ttaccaacat tgataaagtt gcagatgttg
     1861 aagtttttaa ttttgatgaa attccaggac ctcacataac accttataga atgatggaat
     1921 tgaaaaatta tgtaaatgat ttattagcaa gaaaagatat tacaggtgtt gtaataacac
     1981 atggtacaga tagtttagag gaaacagctt attttctaga ccttacaatt gacaacatca
     2041 aacctgtaat agtcacaggt gctatgagaa gtagttctga acttggatat gatggttcaa
     2101 gtaatttatc tgcatcagtt tgtactgcag tatccaaaga tgctatggga aaaggagtcc
     2161 ttgtagtttt aaacaatgaa gtattattag cttctgaagc tactaaaaca aatactcttt
     2221 ctctaaatac atttaaatct ctaactagtg gtccattggg aataattgac tgcaacgaac
     2281 ttgtccttac aagagatatt gtaaatagaa caattataga tactgataag gtagaatcta
     2341 aagttgcctt atttaaagca tatgtaggag aaaatgctga ctttataaga tttgctgttg
     2401 atagtggtta taaaggtata gttattgagg caatgggcag aggtaatatt cctcctcaaa
     2461 tgctttctgg agttgaatat gctagagaaa aaggacttcc tgtagttata gtttctagat
     2521 gtcattcagg tagagtattc gatagttatg gttactttgg ttctggtaga gatttaaaaa
     2581 atcttggttg tatatttggt ggggacttac caggtcaaaa agctagaata aaacttatct
     2641 tagcacttgg aaaaactgat aaccttgatg aaatcaaaga cttctttgaa aaaggaatct
     2701 attgttaatt ctaataactt ctatttaatt atttattgat atttcaacat ttttaaatca
     2761 aaatctaata cttatgtcag ttaaatgtca gcaaaatatt ttatccacaa attttccaca
     2821 acttatcccc aatattatcc acaagaaaaa aggtagcact tatattagta gctacctttt
     2881 atttattaat cttcatcttt ccagcttttt atccaatttc ctatttctat tattttttct
     2941 tcagttattt cttctccatc atgtaatttt gaaattagtt ctgataatgg attgttatgt
     3001 agacctccaa aaattctttt tccctctatt ttgagatatt ctttctcttt tattaatact
     3061 ctatagtgta catgtttccc aattctctgc gatatgaaaa aacctttttt agttaatcta
     3121 gctaaaagtg taattgtagt tgtatgcttc caagcatatg tttttctatt taatctgcaa
     3181 catcttttga tattacagtt ttataaccag tattccatat gtatttcata atttttaatt
     3241 ctgcatctgg taatttttta ttcaacataa tatattcctt ctttcaattt atgtcacttc
     3301 ctcaaactta tctagcttaa attaataata ggagcttttt atcacaaaaa ctacaggtaa
     3361 atttttctat atattctacg tatttagtct gtttttaagt gtgaaattac gttcatacct
     3421 ataaatttat tcaattttca aacaatcttg tttaataata actaattcta ttgtattatt
     3481 tagttataga ataaaactaa atcggcaaaa ctagagaaat atagtgacgc aaagctatag
     3541 ggactaagac ttatataaat atcttatgag ttatgtcagc cagttgccaa agagatattg
     3601 ttctttttgt ttttatgaaa gttttttaag gggataataa tttatatgtt tagagataaa
     3661 atggataaat gtacacatat attgactgct tatattagca gttcgtatga ttattgtaat
     3721 tttttggata caatttacat tccatatagt taatataaaa ctcaattaaa ttatgacctt
     3781 ggtttagata gttcattatt tacattccat atagttaata taaaacgtaa ttcattagaa
     3841 cctttaagtt gtaaatctct catatttaca ttccatatag ttaatataaa acgagcatca
     3901 aacacatgaa aatcggaaag cctacccatt tacattccat atagttaata taaaacgtgg
     3961 tagcgttcaa aatataataa aaggtgctac aggatttaca ttccatatag ttaatataaa
     4021 actagcgata gggttaaata acccaccata atctatcgaa tttacattcc atatagttaa
     4081 tataaaaccc caaaataaat tgagtatttc caatgcctac acatatacaa atctctcaaa
     4141 tttgcagtga accatgagta gtgcaattga taacacttat cacaagtcct caatgcctta
     4201 tatttcaact gttaagcctt aatttcttca aaaaatcgaa cactgcaaaa tttctatgac
     4261 tttattatat cataaaaata ttatttttga atatctgtac caatttgtga tataataaaa
     4321 gcaagaagaa ctacaatcta ttttgctgta gagtggagtt cataatttag aaattactta
     4381 gttttatgga atctgatttt taaatcaaat tcccaaccac tcttagttgc cgctttgagt
     4441 ggttttttac gctttctaaa tagtttgcta gctatataaa ctattaagct agcagataga
     4501 cttgctagta tgccttgtaa aaaattatcc atgtatattc acctcccttc ttagcgtcgg
     4561 gaggataatt ttttgataca tgaactccac tctataaatt gtagattaca tcttcttgct
     4621 aaaaatatta taacatataa ttcttacata ttttacctat ttattacttt ttacattata
     4681 ataatattct tttaatcaaa aaaacaccta ctattaaagt aaatgttttt aagtattttt
     4741 aattttaaaa tccatatagt tcatatgaaa ctaaatataa taactcaaca tatgagcaat
     4801 atcgagacat ttacatacca catagttaat ataaaacgaa aataaaaaaa ttaaacttat
     4861 aaaaccctta ttaatttaca tacccactag ttatttatat tcaaaaaaat cttctccatc
     4921 tgggttataa aataatattc tacttgccaa tccttgttca tatttataat tttctttatc
     4981 acctacataa gaaaatgtta atgtcatgct tcttgatgga tctaatgaaa ttatatctaa
     5041 ataaatagta catttttctc ctggctttaa tacaccgaaa ctattaaaat ccatttttat
     5101 tttagcatat tgccccatat ataatgttga attagcttct ccataaactt ttaattttgt
     5161 tacatctaaa ttttgtgtac ttgtattttc tatatttata ggaactgttg ggtctttata
     5221 tttttcagta taatattcaa ggttatcatc ataacattca agataacttc ctttatcttg
     5281 ccaaattatg tgctttgaat caaaatcaat ctttaatcca catattatat ttttatttga
     5341 agacggaaga ctaatattat tgatggtatt ttcacactct agcataattt tatcacttag
     5401 atttcctatc ccagtcactt ttgcagcatt tttaagtata tctttattac ttccaatatc
     5461 aactagtaca ataggattat ttttagccat tggtgaagca actaaagcat aaactaaatc
     5521 agtaccacta gctatataaa attcttttgc gccattatag aacttattta ctattttttt
     5581 attagtatca tatctatcta ctccacctaa tctagttgaa ttagtatcat taactagttt
     5641 gtcactcatt gatgaagtac cacctattac ataactttct acaccagttg tattaaatgg
     5701 aacacttttt ccatcagtta atactatagc tgctttatct ctaactgcta caggtgcagc
     5761 actcattgca tcaggctctc ctttaaaagc attagtcaat ataactttat ttactttgtt
     5821 aattgagttt atttcttttg caacattata gcttgtctta attctatcac taccttgaag
     5881 tcttttagcc tctattcctt tatcttttag aagagtttct gttgctttat caatagaact
     5941 ttctccacct attatgtata cctttttagc tttctctact cttttaagag ttgcattagg
     6001 tatgttattt ttcttagtta aaaaaattgg tgcatttgtt gctcctgcaa gtccactcgc
     6061 acttaatcca tcagccattg tgctgtctgc attaattaat atagctgtag tataattttg
     6121 ttcatcagct ataagagcag ccgtttcata tttatctttt ccttgaatag tataaattgt
     6181 atcaagtgca ttagctgata taggaccagc agcaaacatt gacacggcta accctagtga
     6241 taataatttc tttaatttca caatatctcc cccttttata tatgaaatac tatatatctt
     6301 atcataattc tacattaaat tttcttttcc tcctttaacg atatttatct tcctgttaat
     6361 ttaagtgtat acaaatacgt gttataatgt aactgtaaag aattaaaaag ataatgacat
     6421 aattagagca gtccaactgt tcttttataa aactaactta taaagggagc gtacatatat
     6481 gaatatcaaa tcagctttta taagaaaaag atgggaaaaa ttctatgtat atgtggaata
     6541 agaaactggc aaaaagaaac aaaaaagtta tggaagctat aaaaagaaaa aggatgctga
     6601 aaaacattta attgaaataa agtctactat aaataataat aagtttgttg ctccaaaagg
     6661 tatgacattt gtagaaagat gttataaata tatagacatg aataaaaata attggtctcc
     6721 atatactgtt acaaatacaa aatcttgggt taaaaattac atagaacctt ttttaagaat
     6781 ctaaaactca cagaagtaaa tcctagttca gtacaagctt ttgtaaatta taactttaat
     6841 aattctacct ttactagtgc taaagtaagg tatagctttc taagttctgt aataagggaa
     6901 gcgtatagat taagagaaat acaagaaaat tcatgtgatt ttataaaact tccttctaaa
     6961 gaaaaatatt ctacaaatga aatatacaat agagaagaag tattattgct aatagaaaag
     7021 ttacttggta ataatataga aatacctatt cttttaatgt tacttctagg tttaagaatg
     7081 tgagaagcta ctgggcttag gtggtcagat gttgacttag ataacaatct gataaatgtt
     7141 aatcaaattc ttgtctattc taatggcaaa atatctttta aagagccaaa aacaccaaaa
     7201 tcaaaaagag ctttatcagc tccaaaagaa ttaattgaaa aattgaaaat tgaaaaacta
     7261 agacaaagca aattaaaatt gtaaggtatc ctggaaaaca gaaataactt agtttgtcta
     7321 aacaccaatt taaatccgtt tctaccttct tcactaagaa aaacgtttca caattttaca
     7381 aaaaaaacaa tttaaaaaag attagaatac atgatttaag atatacaagt gccactctgc
     7441 tgcttttagg gggaactaat atgaaagttg tttctgaaag attgggtcat acagatataa
     7501 aaataactat gaatagatat tctcatgtac ttaaagaaat ggacaaagaa gcttccgaca
     7561 atctaagcga acttctattt aaatagtaaa aactgactgt cagttaaatg tcagttaaac
     7621 ctaaaaaaat aggttttgtc agttgattat cagtcaatta ttttataata gtttcactta
     7681 aaaacgtcta tatctacaca ttttactatt tgtttatgct tattaatatc tatctgttat
     7741 tattaattct aatagcttct atttaattat ttagttcagt ttacaggaaa ctacacacaa
     7801 accataaaat aggatgttga caatataaca gatagtatgc tcctgtaaaa ggtaacatac
     7861 tatctgttat gttgtctgga tttccaaatg gttacccttt ggcacacgac tttgctatct
     7921 ccttggtaaa ctaaaagttt gtaatctaaa atcctaatcc atctctataa aatttaattg
     7981 acttctccat actttttaca cctaaacata taaaagtaat tttattcact taatgacctc
     8041 cataattccc aaattagcta tttggaactt aactaaaaaa tcctaatata agggcaacta
     8101 ttataatggc aatcggaata catactgtgt aatcctgttc catagagtca tcatctgaat
     8161 gtagttttac aattcgcaat ttagcaatca gtttgaagca gaaatagcct attattgttc
     8221 caattacgtt cgtgagtaag tcgtctatat cggttgctct gtgtaacgta aaaagctgaa
     8281 tagtttcgat aaaaagagat aatccaaaac caatcaagaa tgtatttctc aaacgttcaa
     8341 aggttttgct tataagcgga cataaaaaac ctaatgggac aaaaagtaaa atattcaaaa
     8401 taaaactcaa actaaatcca tcactaaaag gaataagatt tatgtttgga ttaaagaacg
     8461 tttcccctaa ctgtgacagt cttatatatt caccgaatgt aggtatacca acaatatgcg
     8521 atagtgttaa acataagtag taatacattg ccagtgataa aatagatgtt ttgacctttg
     8581 gaatatcact atttttcttg aatattacaa ctaagaaaat accaatcaag ataattccca
     8641 acaagaaaat aggattaatt aacaataaat ctttgatagc ttcaaacaga ctgagttgaa
     8701 tttctacacc tgattgatta tttgttacag tctccatttg tacatacctc ctcataacat
     8761 ttcaattatt tctaaaaaaa atagaagata aatcttaaaa tcatcctatc aaaattttta
     8821 aatttgcctt tatcagttta taccaccagt taattcctgt cttacttctg tatggtattc
     8881 ttgggattct ttcatcaaat tagacaaaaa atggttacca tatcaataaa ctggttacgc
     8941 tccatatctc ccgaagcaac catctcctga gaataaatcc gtatcaggaa tctccacatc
     9001 tactgcacct atcacaagtt atttgataac atcctcaaac aatcccaact tggatatatc
     9061 gacaacaccc attagaatgc tactgctgac catccttgtt ttcctggtga taattttaat
     9121 caactggtaa gttgaaacgc ctattttttt tgcaagttcc tttttagaaa catggtagct
     9181 cttatatgaa tctgaatttc gttgtctggg tgtttcatat aaagcataga tacgcacctt
     9241 ttttgcctat tttcattata tagaaaaaaa tttcaaaata caataattat atttttttcg
     9301 tttaagctta gagcagttcc ttgatgaaca acactttgga aactgaatga tttacatacc
     9361 acttaataca ttaactcgat ttaacattaa ttataa
//
LOCUS       DAFKMX010000155         9340 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFKMX010000155 DAFKMX010000000
VERSION     DAFKMX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.155"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            101..730
                     /locus_tag="LZB08_002928"
     CDS             101..730
                     /locus_tag="LZB08_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316412.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            731..1513
                     /locus_tag="LZB08_002929"
     CDS             731..1513
                     /locus_tag="LZB08_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316413.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1506..2384
                     /locus_tag="LZB08_002930"
     CDS             1506..2384
                     /locus_tag="LZB08_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316414.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIVNDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2417..3376
                     /locus_tag="LZB08_002931"
     CDS             2417..3376
                     /locus_tag="LZB08_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBM7316415.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3692..4384
                     /locus_tag="LZB08_002932"
     CDS             3692..4384
                     /locus_tag="LZB08_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316416.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4394..5398
                     /locus_tag="LZB08_002933"
     CDS             4394..5398
                     /locus_tag="LZB08_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316417.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5646..6497
                     /locus_tag="LZB08_002934"
     CDS             5646..6497
                     /locus_tag="LZB08_002934"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBM7316418.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSIIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6601..7347
                     /locus_tag="LZB08_002935"
     CDS             6601..7347
                     /locus_tag="LZB08_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316419.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7347..9263
                     /locus_tag="LZB08_002936"
     CDS             7347..9263
                     /locus_tag="LZB08_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316420.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3621 a          881 c         1660 g         3178 t
ORIGIN      
        1 attgttttat taagaaaaaa ttaatatatt gacaaaattt aagtgctcat ttaaactatt
       61 aattgataat aatattcaat attaaataat aggaggcgtt atgaataaat atcttctaat
      121 aaatccagtg gcagaaagaa tgtatggaga aaagtttcac cttattaaag caaatttgat
      181 aaaaaagggg tacataatag cagaatgtga accacagtta gaatatgtaa aaaagcagta
      241 caaagagtat acaaaaaaga ctgaaaatac aatgttggat tgtagatgtc cagaaagtat
      301 aagtctttta aaaagaaata atttgataaa tgattttgaa gtacctatga tagaaccaat
      361 attagtaagg acttgtaggg ttttatacga taaatatata aaaagtaaag atgatatgtt
      421 ggtagtaacc tgtccatgta cacaattaag agactttgca agtgaaaagt ttaaggataa
      481 gggtaatgct atgttttata catggaaaga gtttgctgaa caagaaggtg taaaatcact
      541 tggaaaaata gatgaatcac caattccatt aggatatttc aaagatactt ttgaaaaagt
      601 tcttgaagta tcaagtgaag acgaaatatt aagtgaaata caaaaaataa gaaatggttt
      661 tgaagataag tatgacatag tagaaatgtt ttattgtaaa gatggttgta ataatggaga
      721 tggtttgtaa atggtcaata aatttaataa taagttttta aggctagtaa agagtgttct
      781 gatatttttt ataattcttt tattatggaa aattacaaat tatcttggta tttggagtga
      841 ttatatatta ccatcacccg aaaaggtata tagtacattt ttaaatatga taagtgatgg
      901 ttcaatattt attaatgtat atgcaagtat gaaaagagtt ttgataggtt ttgcaataag
      961 tactgctata ggtgtgcctt tagggatatt ctttggaatt tatagtggag tatacgaata
     1021 ctttaaatct ctaataaatt ttttaaggaa tacacctccc cttgctttaa taccaatgct
     1081 tatattatgg tttggtatag gagaggaatc aaaaattata attatagttt tagcatcctt
     1141 tttcccaatc tttacaagca cattgaaagg gattaaaaat tgtgactcaa aactgattga
     1201 agtgggtaga gtatttgagt tttcaaagct tcagataata tttaaaataa taataccaaa
     1261 tgccattctt gatattgctg ttgggttaaa gttagctctt ggatatagct ttagagcaat
     1321 tattggggca gaacttgtag cagcttcatc tggtcttgga tacttgatat ctgatggaaa
     1381 agaaatgtct agaacagatg ttgttattgt aggtataatc gtaattggac ttttaggaat
     1441 aattacagat tacatatttt cgattattgt gaagaaggta agtaagggaa agatggtgga
     1501 agcatatgaa taatttagta tgtgactctg gatatataat tagaaattta tataaaagtt
     1561 ataaagttga caatgaagaa catgtagttc ttagtgatat ctctttaaac atagatagaa
     1621 accatataac agtaatttta ggtgaaagtg gttgtggaaa gactaccttg cttagagcaa
     1681 ttgcatcact tgaaggtata aatagtggtg aaattaggtt tattaatgat aataaagagt
     1741 atagacctaa tgttggtttt gtttttcagg agagcagatt aatgccttgg ttaaacgtaa
     1801 gtgaaaatat agcttttcat aatcaaagaa aaaatactat tttaaacaag gtgcttagaa
     1861 aatttaaaca caataaaaga attagcagta aaattgtaaa tgataatttt agaagcttag
     1921 aaacaaacga taatttaaac aaatcaaata aatataatag gaaaagtaat attgatgtag
     1981 aaaaatatct taagatgatg aacttgcaga aatttaaaaa ctcttatcca aatgaattgt
     2041 caggaggtat ggcacagaga gtaagcatag ctagagctct ttctttcaat ccagatatgt
     2101 tgcttatgga cgagcctttt tcggcattag attattttac aaggattgat atgcaaaatg
     2161 aagttataag aatacataag tcaactaata aaggcgtgat atttgtaact catgatattg
     2221 atgaagcgtt aaagataggc aagaaaatag ttgttttcac agacgaaaga aaagtaaaag
     2281 aatttaatat agaagatggt tataatagag atttaacttc agattactat attaaattaa
     2341 aaaaagatat tttaatgaca atgaaatcaa ataaaggcat ttaaagaaat taataatatt
     2401 ttaaggagta aagtaaatga aattaaaaaa gctattgata ggtataacga tattaacact
     2461 tgcaacagca tctttagctg gatgtgctaa aaaaaataat ggagaaaaac tttcagaaat
     2521 aaatttaact tatgtaaaat caccattaaa tgttccttct ataattcaga aacaggatga
     2581 tttatttgga aaggaattta aaaaggataa tataaaagtt aacttccacg aaattactac
     2641 aggtccagag caaacacaag ctttagctgc tggagaaata gacttcttac atgcactagg
     2701 tggtacatca gctttaatag ctgcatctaa tggagtagag ttaaagatat taaacactta
     2761 tagcaggtca ccaaaaggat ttatgatttt gactaataat aattcaataa aatcagcagc
     2821 agatttagta ggtaagaagg tggcaggccc aaaagggact atattacatc aggtattgat
     2881 ttcagcactt gataaagaag gattaagtat ggatgatgtg gaatttgtta atatgggtat
     2941 tccagaggca tcagcagctc tttctgatgg cagcgtagat gctgcattga ttgctggtcc
     3001 agcagctctt aaagctatga agtcaggtag taaacttgta gcaaatggtg aaggtttagt
     3061 agatggtata atagtaactg ctgttagcac agactttgca gagaaacatc cagaaatagt
     3121 tgaaagattt atgaaggttg aaaaggaaac tttagaatat gtaaataata attttgatga
     3181 agctatggaa aaagtagcta aagaagttga tttaagtctt gaagaaacta aagaattata
     3241 tgcttggtat gattttagtt tagatataac agataaagac atatcttcgc ttgaagatac
     3301 acaagacttc ttaataaaaa ataagttaca agagaaaaaa gtgaacataa aagaattaat
     3361 ctataatgct aagtagaaac tatgataaaa taatactaag taagaaatat aataaaagtt
     3421 taagttacct tattaaggta actttttcta ttttgaatta gtaatttcaa tttattttag
     3481 aatgattagt tgataataat gattaattaa tcataaaaag atactttttt atagagtttt
     3541 attaagatgt ctaataatat ggttatactt atgaagtcag taattgtgaa gttagtaata
     3601 tgattaaatt tatatattgt aaaaagatta taatatatta aaattggact gttaatatga
     3661 tgagtaaata ggtatatata ggaggaatta tatgagttat aaagtatata ttgtggaaga
     3721 tgatatatca ataagtacat tacttaaaga ttatattgag aaatatggat ttgaagtgat
     3781 tgttgaagaa aactttgatg atataatgat tacatttgaa agttttaagc cacacttagt
     3841 tctattagat gtaaatttac ctaaatttga tggtttctac tggtgtagat gtattaggca
     3901 acagtcgaat ttacctataa tttttatatc agctagagat ggtaatgtag accaaattag
     3961 agctttagaa agtggagcag atgattatat aacaaaacct ttttactatg atgtagtaat
     4021 ggcaaaaata aagagtcata taagaagggt atatggtgaa tattcaccta aaatagacga
     4081 aagaatagtg gagctagatg gattaaagtt ttatccagag aggttagaac tagaattaaa
     4141 tggtgagaat ctaattataa ctaaaaaaga aggtatactg attgaatgtc ttatcaagag
     4201 atacccaaaa gtagtaagta gagattatct tttagaaaag atatgggatg atattgagtt
     4261 tgtagaggaa aatactttaa atgtaaatgt tagcaggata aggaaaagat ttgaggaatt
     4321 aggtatagat aatgctgttc agactgtccg agggttagga tataaattaa ataagaattg
     4381 gtaaggagaa agtatgaagt tatttttaaa agattataaa ggttatattt ttatatatta
     4441 tataggcata gttatgacaa ttatttactg taatcttatg aagtttatta gttttggaga
     4501 gagtttatat attcttttat ttagttcttt tatattgtta tgttttttaa tatataagta
     4561 ttataaaaat agagaagtat ataatctgtt tgaaaaagga gtacattctt tggaggagtc
     4621 atttttgtat ttaggaagct cattcttggg gagaaatata tcagttattt taaaacaaca
     4681 acataaattg tatcaatcaa tgatacaaga gcataaaaag atacatagtg aacatttaac
     4741 tttcatcaac caatggattc atcagatgaa aacaccaata tcagttataa gccttcagct
     4801 tcaagattat gagggagaag aagtaactga aaatataaga aaagaaatag atagattaaa
     4861 taaagggtta gatatggcta tgcattttgc aagggcagat aattttcaga aagattttat
     4921 agttgaaaaa gccaaattaa atgaaattgt atctagtatt gtaaatgaag aaaaaaggtt
     4981 atttataaaa agtggaatga ttcctaagat tcaagtagac aaatttactt atgtttatac
     5041 agatgtaaaa tggatgaaat ttataatagg tcaattaatt acaaatggag tgaaatattc
     5101 taaagattat agtaactacc taactataaa atctgaagaa aataataaat atgtaatttt
     5161 aagtataata gatgaaggaa ttggaattgt ttctaaagat ttaaaaaggg tatttgaccc
     5221 attttttaca ggagaaaatg gtcgtaagtt tggagaatct acaggtatgg gtctttatct
     5281 agtaaaaaag gtatgtgatg atttaggcca taaagtaagt ataaaatcag aagtaggaaa
     5341 aggtacagaa gtttcaatct actttaataa agatgtaaat agtttaataa aggtgtaaat
     5401 tatctgttac aatagggttt ttagttaatt ctactttttt attatacaat ttattaaatg
     5461 aaatattcat agttaaataa tcaaaattta agagagttta tttgcaaacc ttacatagat
     5521 gtaaggtttg tgtaatgttt gcagatagtt aaattttcta atctaaatta caattaatat
     5581 aagaaataac ataagagaaa taacataaaa aaagaaattt tgtaaaaaag gaaggatgtt
     5641 tattgatgag tttagatgtt atttttatat tgaaatcagt cataatagct atagtagagg
     5701 ggcttacaga atttattcca gtttcatcta caggtcatat gattttggtt ggtaatttaa
     5761 tagattttaa ggggcaattt gctgaaatgt ttgaggttgt aatacaactt ggggctatat
     5821 tagcagtagt agtactttat tggaaaaaga taaaagatag cattatagaa ttttttaaat
     5881 ttatatttac tggcggaaaa gaaggaaaga ttggttttag atttggaatg aatgtaatta
     5941 taggttgtat accatttgcg attataggag ttctatttta cgataatata aaatcattgt
     6001 ttaatttaca atctgtaatt ataggattta tagtgggtgg tattttatta ttagtagtag
     6061 aaactttgtt tagaaaaaag aatcattcca cagataatat agataaaatt actccaatac
     6121 aagctttaaa ggttgggaca ttacaagttt tgtctgcatg gccaggtatg tcaaggagtg
     6181 catctactat tatgggtgga tggatagcag gacttaattc tcctacagca gctgagtttt
     6241 ctttcttttt agcagtacca gctatggtgg cttcttcagg aaaagattta tttgaatttg
     6301 attattcaat aatgacacca acattgtgga ttgctttggt agttgggttt atagtagctt
     6361 ttattgtatc aataatagtt atggaaaaat tcgtgaattt tttaaagaaa aaaccaatga
     6421 gagtgtttgc tgtatacaga ataatcatgg gtgtagtact tgcagtatta gcttttacta
     6481 acataattag tgtatagaaa aaatagattt attatctata aaaattaaac taagataaat
     6541 tataattaac aattatatat atgttatttc aaaaattaaa ttaggaagga tagttaaata
     6601 atgaaaattt taactgtaaa taatttaagt aaagtatatg gtaaaaaaat aattttcaac
     6661 gcattaaatg acataaattt tagtatagaa gatggagaat ttgtaggaat tatgggtcca
     6721 tctggtagtg gaaaaactac tcttttaaat atgatttcta ctattgacaa gccaactaca
     6781 ggaactatgg aattaaaggg caagaatcct ttattgttaa gaggtgagga acttgcatta
     6841 tttagaagaa gagagttagg ttttgtattt caggatttta atttattgga tacattaact
     6901 ataggagaaa atatagtatt gccattgaca ttagataagg tcagtgtaaa agagcaagat
     6961 gagagattga atgaagtatc tacaatatta ggtataaaag atttattagg aaaaagaact
     7021 tttgaagtat caggtggtca agctcagagg actgctattg ctagagcatt gattaacaat
     7081 ccatcaatac ttttagcaga tgaaccaaca ggtaatttag attcaaagtc atcaaaggtt
     7141 gtaatggagc tatttcaaaa gataaataaa gaaaataaag ttactactat gatggtaact
     7201 catgacccat tagcagcgag ctactgtagt agaatcttat tcataaaaga tgggtctata
     7261 tataatgaaa tatataaggg tagtagtaga gagcagtttt atcaagagat aatggatgta
     7321 cttacattat tgggaggcga taactaatga atataaaaca gtttgctatt aacaatattt
     7381 caagaaatat taaagcgtat ttaggatact tagcaagtat tgttatatca tcttcactgc
     7441 tattttcttt tattatgttt acaagtcatc cagatttaga tgtatctcaa tttcctgatt
     7501 atttaaaaga aggtctcaag atgagtaaga taattgcata tctattctta ttcttctttg
     7561 tgttttattc agttagtgtg tttcttaaaa gtagatataa agaatttgga atactttata
     7621 ttacaggtat atcaaagcgc caagtaatga aattaatctt tatagaaaat atgataataa
     7681 atatagtatc ttctgtaatt ggaataataa taggcttaat ctttgcaaag atatttttag
     7741 tggttatgtc tactttttta gaattatcac cattaaaatt ctatatacca tttaatgcga
     7801 tattgtctac tttaatctat tttatgattt tagctgtatt aacatcaata tttacttctt
     7861 ttatagttaa agaaaatcag gtgttgaagt tacttaaagg tacaaaaact ccaaaaccag
     7921 agcctaaaac atcactaatg cttgctatat taagtatttg tttgtttata atagcgtatt
     7981 ataatgctat tacctcaacc gaccaatata caacatatct gcgcttagtt ccagttacat
     8041 cccttactat agtagcaact tacttgttct tttctcaatt tagtgtattt tttatgaaga
     8101 agctaaaatt aaacaagaga ttttatagaa aaaatacaaa tatgctttgg atttcgaact
     8161 tgatttataa ggtaaaggat aatgctagaa tatttttctt gataactata acttctgcag
     8221 tggcatttac tgctataggt acagtttatt ccttttggaa agatgtagag cgtcaaatta
     8281 atttaatata tcctaacaca atatattatt caacaatgac attgcataat gaccctaaaa
     8341 agcctgatag caaagaaaaa gacaaggaaa gaatgatgtt tatagaaaat aatctaaaaa
     8401 aagagaatgt agactataca aggattaatg gtgaatttaa aactgtattt cccaagaaaa
     8461 gtgattttaa tgtcagaatt atgaaagaat ctaagtactt agaaattaca gaaactatag
     8521 gggttaagcc tataagtttt gaagataatg aatgtatatc attattatca tcaagtttac
     8581 caggaaataa gaacgtcaaa gaaaatatta ttgtagggaa tagaggttta aaggtggcaa
     8641 aacaagtgga agaatgtgtt atgccagcat attacaaaaa tatgtatgtg gtaaaggata
     8701 atttttatga tagtatagaa tcaaaattta tagtggatag atttactgca tttgaagtag
     8761 aggattcaag taaaactata gagatatgca gagaatttga aaataaattt aataatgagt
     8821 cagggatgca accatatttg tttttctcta aagaattaat gataggaact gggaaattaa
     8881 tgtattctac atttatattt ttagcaatat ttataggttt aattttcttt gttactacaa
     8941 gtagtttttt atataataaa ttttatatgg attgccaagt tgataagaaa aagtatgaac
     9001 agttaaataa attgggtatg acatataatg agattaaaaa agcttcaaca attgagattg
     9061 gaatagtatt tttgtttcct tatgtagtgg ctgttataca ttcaatattt gcacttactg
     9121 cacttaaaaa ttcttttgat atagaagtag catcatcagc tgttttggta atgggaagct
     9181 tgttcttggt tcaaatagtt tattttttaa ttgtaagaaa taattattta aaagagataa
     9241 ggttaaattt agtcaatttt tagttactat tattaaatga taataaaaag ggtttagaat
     9301 taattaatat tactggaatt atagatgttt attttttcag
//
LOCUS       DAFKMX010000156         9334 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFKMX010000156 DAFKMX010000000
VERSION     DAFKMX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.156"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(317..1033)
                     /gene="dapD"
                     /locus_tag="LZB08_002937"
     CDS             complement(317..1033)
                     /gene="dapD"
                     /locus_tag="LZB08_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBM7316421.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1257..2006)
                     /locus_tag="LZB08_002938"
     CDS             complement(1257..2006)
                     /locus_tag="LZB08_002938"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBM7316422.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2073..2960)
                     /locus_tag="LZB08_002939"
     CDS             complement(2073..2960)
                     /locus_tag="LZB08_002939"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBM7316423.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3077..4078)
                     /locus_tag="LZB08_002940"
     CDS             complement(3077..4078)
                     /locus_tag="LZB08_002940"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBM7316424.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4503..5384)
                     /locus_tag="LZB08_002941"
     CDS             complement(4503..5384)
                     /locus_tag="LZB08_002941"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBM7316425.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDALL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPFVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5707..6900
                     /locus_tag="LZB08_002942"
     CDS             5707..6900
                     /locus_tag="LZB08_002942"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBM7316426.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7106..8224
                     /locus_tag="LZB08_002943"
     CDS             7106..8224
                     /locus_tag="LZB08_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBM7316427.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAIN"
     gene            8355..8600
                     /locus_tag="LZB08_002944"
     CDS             8355..8600
                     /locus_tag="LZB08_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBM7316428.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8587..9333
                     /locus_tag="LZB08_002945"
     CDS             8587..9333
                     /locus_tag="LZB08_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBM7316429.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
BASE COUNT         3361 a         1353 c         1425 g         3195 t
ORIGIN      
        1 aaaatttagt agttcatttt attaaattat agaaagatta atggttttca ctttattaaa
       61 ttataaaaag tttaatagtt tattacattg atatattgaa aaattgaagg aaataaaaat
      121 acttgtcagc aagaaataaa aatgatataa gaatgaataa gagaattgta aaaaaataaa
      181 atgattataa ataaaagata aaaatattgt aaaaaagagg gtgcctctgg tgaattaaat
      241 tcacacagag acaccttttt aattaatttt ttaaattagt atattattgt ttaattaata
      301 gcatagtact atttaattaa tctaaatttc taaggtcttc cataagctta gttttgtcag
      361 ccgttttttc atctttcatt tttataactt tagcaggaga accagcaaca acggcaccag
      421 cttctacatc agtagttaca acagcaccag ctgcaacaac agcaccttta ccgattctaa
      481 caccttcaag aataactgca ttagctccta ttaaaacatc atcttcaact ataacaggag
      541 tagcagaagg tggctctaaa acaccagcaa caacagcacc agcacctaag tgaacatttt
      601 taccaagagt tcctctagct ccaataacag cattcatatc taccatagaa ccttcaccaa
      661 taacagctcc tatatttata acagctccca tcattacaac agcatttttt tctatagtaa
      721 ccatgtctct tattattgca cctggctcaa ttcttgcatg ttcacttaaa gtatttttta
      781 gaggaatagc agaatttctt ctatcatatt ctacatgagt atctgttata tcatttccat
      841 gtttatctaa tacttccatt atatcctcat agtcacctac aaatatataa gaaccattat
      901 ttccaaatac cttaaattta tcagttgatt ggaagtgtaa agagttagta tttacatata
      961 ccttaacagg tgttttcttt ttaacttctt ttatatacct tgctatttca taagcatcgt
     1021 ttaaatttac catttgttaa ttctccttgt atttcattat atttctttta ttatatactt
     1081 atttgtcaat tttagtatat acttacttct tattatttat attttatttt agttagtttt
     1141 tattgtatag acatagtatt aaataaattt attatatatg tttgatttgt attgcatatt
     1201 attatatata tattgtatat gtttaattta tattgtgtat tattatattt atactattat
     1261 cccaacatgc tgtccatatt ataaaatcct ggtttttgct taactaagta tttggcagca
     1321 gctatagaac cacttgcaaa gataccacga gattgagctt gatggcttat tgtaagaacc
     1381 tcattgtctc cagcaaatag agcatcatgt tcgccaacaa tatttccacc tcttatagca
     1441 tgaataccaa cttcatttgg ttgtcttttt gcactacgac catatctacc ataattattt
     1501 tcaacgtttg gcaaaacctc ttttatagcg ttagcaatca taacagctgt gccacttggg
     1561 gcatctactt ttctgttgtg atgtttttct attatttcta tatcaaatcc ttctagtaat
     1621 ttagctgcat ctttaaccaa ttttactaat acatttacac caagagacat attatatgat
     1681 tggaatacag gaataacttt agccgcttca cgtattttgt tcatttcgtc atcattatat
     1741 ccagtagtag caattactaa aggtgtttta gtctttaaaa cgtaagataa gatattatct
     1801 aaattagagt gatgagaaaa atctataact acatcagctt cctcaactat agtactcata
     1861 tcttcataaa ttttaaggtc gccttcatag ctatccagac ctctagctgc aacacaaacc
     1921 aattctaatt cattatcttc tcttatagta tcggctaaaa tcttacccat ttttccatca
     1981 taaccagttg ctataacttt taacataatt tcctcctatg attaagggct aacttttgac
     2041 caaacttact ttttaccatg cattaatctt tactatgcat taacttttag cccaaagtta
     2101 actaattctt tctttaatac ttctaaattt gctgggtcca tttctgctag aggtaatctt
     2161 aaatctccaa cattgaatcc caataaattc attgcagtct taactggtat aggatttact
     2221 tctataaata aagctgcgat tagagcatcc ataccaagtt gtaattttct agaaccttca
     2281 atatcgcctt caaagaattt agctaccaaa tcgtgagttt cttgagggca aacattagca
     2341 agtactgata taactccttg acctcctaaa gataataaag gaagtatagt atcatcatta
     2401 ccagagtaga tagcaaagtc atctggaact aaactagcta tttctgcaac ctgtcctagg
     2461 tctccactag cttcttttac agcaactatg ttatcaattt tagcaagttc aactataagg
     2521 ctaggactta tatttacaca agttcttcca gggacattgt aaagtataat tggaagttta
     2581 acactgttag ctattgtttc aaaatgacgc ttaagtccag ctttattagc tttattgtaa
     2641 taaggtgtta ttattaatag agcatctact cctaattttt cagcttcttg acttaagtta
     2701 actgagtgca tagtatcatt agaaccactt ccagctataa caggaactct tttattaact
     2761 ttttcaacaa catacttaat agtagcaagt tgttcttcat cagtcatagt attagcttca
     2821 ccagtagtac cacaagatac tatagcatca gtaccattag ctatttggta ttcaactaat
     2881 tcacctaatt tgtcaaaatc aactttgtta tcctttgtaa atggtgtaac tagagcaaca
     2941 gcagaacctt taaatatcat ataaaatcaa tcccctcttt tttatttttg tgttttatat
     3001 tacagttact tctaatgctt gtttttgagt tactattaat attttacaaa attatatatt
     3061 tatacaagtt tgtatattat actaaatcgt atgataaagc catttcagct atttgaattg
     3121 cgttagttgc agcacctttt cttatattat cagcaacaac ccaaagattt acaccattat
     3181 caacactaaa atctcttctt attcttccaa caaatacttc atctctatca tcaaattcta
     3241 cagcagttgg atattcgttt ttagcaggat tatcaactac tacaacacct ggagcttctt
     3301 caagtgtttt tactaaatca tctaactcaa atggattgtt aaactcaaca tttatagatt
     3361 cactatgacc attttttact ggaactctta cagtagttgc agttattttt aaatcgtagt
     3421 tatcaagtat tttcatagtt tcatttacca ttttcatttc ttctttagtg tatccatttt
     3481 cagtgaatga atctatatga ggaagacagt tgtaagagat ttgatgaggg tagaatccag
     3541 taggattacc gttcattcct ttctctaaat cttcaacacc tttaacacca gaaccagata
     3601 cagcttggta agtagagtaa acaactctct taattttgta tttatcatca agagctttaa
     3661 ggggaaccat agcttgtata gtagagcagt taggattagc tattataccc ttatgatttt
     3721 ttatggcttc tggattaacc tcaggaacta ctaaaggcac atctggattc attctccatt
     3781 gactagagtt atctactact aagattccat ttgaagcagc tattggagca tacttttcac
     3841 taatacttcc accagcagag aataatgcta tttgtatgcc tctatcagta aatgaagttt
     3901 cctttaattc ttctactata tattctttac cacaaaacat tactttagaa ccagctgatt
     3961 tagaagaaga gaataaaaat agattttcta ttggaaaatt tctttcttct aatactttta
     4021 aaaatgttct acctaccatt ccagtagcac caactattgc tacatttact tttttcatat
     4081 aaattcaccc caattttaaa ttattaaaac attattttat tttagttata caagtaagat
     4141 atgttaatat ttttatatat ccaaaaactt taatattata ttaacaactt taattatata
     4201 caaaaaacgt acaaaggtat actttgtacg tctaatttat cactcaaagt aaaccctgta
     4261 gctcatcacc tacaaaaata agtgacagta ttacacatgt tatgtgcaat cccagaaaat
     4321 attattgcaa gaaatatttt cttcggctgt aaatcctttt tccaatcatt aacacctgcc
     4381 agctactatt ggatactctt agttacagct acctctaccc taggttaata ggatattaaa
     4441 atttgattat tctaataata atacatattt aagaaaaaat caacttattt atataaatat
     4501 tgctaaaatt taaatccaaa atttgtcaat tcttgtttta acacattaag attagtttct
     4561 tccatttcag ctagaggaag tcttaaatca ccaacattaa aacctaaaat gttcatggca
     4621 gttttaacag gaactggatt tacttctata aataaggcag caattaaagc atccatatca
     4681 agttgtaatt tttttgagcc ttcaatatca ccttcaaaga atttagttac taaatcatgt
     4741 gtttctttag gacatatgtt agcaagtaca gagataacac cacttccacc taatgataat
     4801 agtggtagta tagtatcatc attaccggag tatatagcaa agtctttagg tactaattta
     4861 gctatttctg caacttgagc taagtctcca cttgcttcct tcacagcaac tatattatct
     4921 attttagcaa gttcggccac aacaaatggc tttatattca ctttagttct tcctggtacg
     4981 ttgtataaaa taattggcaa cttaacgcta tttgctattg tttcaaagtg aagtttaaga
     5041 cctttctcat tagtcttatt atagtaagga gttattatta aaagagcatc cacccctaat
     5101 ttttcagctt cttgactcat atgaacagac aacatagtat tgtttgaacc agtacctgct
     5161 ataacaggta ttctcttatt aactttttca actgtatatt ttattacagc aaaaatttca
     5221 gattcactca tagtagtcgc ctcacctgtt gttccacaaa caactagtgc atctgtgcca
     5281 ttctcaatgt gatattcgat taattcacct aatttttcaa agttgacatt attatcctct
     5341 gtaaatgggg taactaaagc aacagcagaa cctttaaata acatataatc aaatctcctt
     5401 ttttatctaa atttaatttt ttaagtacaa taataattat agtacaaatt aattaatttt
     5461 aataggtata gttaatatct taactgttta aataagatat ttatattata ttaatattag
     5521 aaaaatttta aataaatgtt ttattctcaa aatatgcata ttaatttaat aaaatatacc
     5581 aaaatggaaa taaaaaataa attttatgag aatttaataa ctaataatta atatatttag
     5641 gttataataa atatgtgcgt aaaatctaac aataagcaat aataccgtaa atttaggagg
     5701 aataatatgg atactttaaa ggaacttttt aaaataggaa gtggaccttc aagttctcat
     5761 actatgggac cacaaagagc agcagagaga tttaagaatg aaaacccaga tgcagaaagt
     5821 tttagagcaa tattatatgg tagtttggca gccacaggga aaggtcattt aactgactat
     5881 ataattgaaa aaactatagc acctaaaaaa gtcgaaattg tttgggaaga agatattata
     5941 aaagattttc acccaaatgg gatgaagttt gaagctttag ataaagataa aaatgtaaca
     6001 gctgaatgga ctgtatattc tgtaggtgga gggactatag cagaagaagg tcaaagaaac
     6061 agtaaaagca atagtatata tcacttagat actatggatg agatagtaaa atggtgcaaa
     6121 gaaaataata agactttggt agattttgtg ttggaatgtg aaccaaaaga cataaaagat
     6181 tatataaaaa ctataaaaga tgctatgaga aaatctattg atgatggact aagtacagat
     6241 gaaataatac caggcaagtt attattaaaa agaagagcaa gtacatttta taatgcatat
     6301 aaaaaagata agagcttctc aacacttgta tatgcatatg cactagcagc ttcagagcaa
     6361 aacgcttctg gtaatataat agttacagca ccaacttgtg gttctgctgg agtaatacca
     6421 ggaatattct ttgcaatgca ggatttctat aattatgatg acgaaaaaat aattgaagca
     6481 ctacttgtag ctggaataat aggtaatata ataaaaacta acgcttcaat atctggagca
     6541 gaggtagggt gtcaaggtga agttggagcc gcttgttcta tggcagcagc agcagtagca
     6601 tacttaaaag gtggaactat agaccatatt gaatatgcgg cagaaatagc acttgaacat
     6661 catttaggaa tgacttgtga ccctgtatat ggatatgttc aaattccatg tatagaaaga
     6721 aatgcaatgg cagctcaaag agcttatgat gcagctaatt atgcattatt aactgatggc
     6781 tcacattctg tctccttaga ccaagttgtt gaaacaatga aagaaactgg tatagatatg
     6841 atggataagt acaaagaaac agctaagggt ggacttgcta aacatttctt ctcttgctaa
     6901 aaataaaatt ttccaataat aattcctctg ttttgattta tgaaacaggg gaattattat
     6961 taatggtgaa ttatttaata atgcaaaata tttttatatt caatttagct ttttattttt
     7021 aattagtata acatgctata taatatttta attataaaga aatatagaaa tattttttaa
     7081 taaattagaa attggaggag attacatgag agaggcatca tatttacaag aacaacttta
     7141 taaacatagg aagtcattaa atcctattgc agagattggg agaaaagagt ataagacttc
     7201 aaaatatata agagattatc ttgataaatt aggaattgag tatgaagttt atttagaaac
     7261 aggtatagta ggtgtaatag aaggtaaaag acctaaaaaa gatattgctt ttagagctga
     7321 catagatggt cttcatagtg aagaaggggt aaaacattta tgtggtcatg atggacatgc
     7381 tactatatta cttggattaa ttgagtattt aaatgataat aaagaagact taaatgataa
     7441 tatagtattt atattccaac cagcagaaga aggtcctggg ggagcaaaag ctttaataga
     7501 tgaaggagtg cttagaaaat acaatattga tgaaatatat gggcttcata tatatccaga
     7561 gttaccagaa ggttatgtag gtacaagaga gggctatttt atggctcaaa taggagatat
     7621 tgacatagaa atagtgtcta aaagtggaca tggggctatg ccacaaaatg gtattgatgg
     7681 tatagtgata gcttcaaact ttgtaagctc acttcaaact atagtgtcta gaaatataag
     7741 cccaatagac aatgcagtgt tgactattgg tagaattgaa gggggagcta gaagaaatat
     7801 aatagctgaa aatataaaga tggaaggtac tatgagatgt ttcagcccag aagtgtatga
     7861 gaaaatgaag cttagagtta gagaacttgc tagagggttt gaattggctt ataactgtaa
     7921 ggttaatata aatattatag atgattatat tgctgttaaa aatgacaagg atttgtatca
     7981 agagtttgta gaggctattg gaagtgatac tgttgtagag ttagagccac ttatgatatc
     8041 agaagatttt tcttattatc aaaaagaagt tccaggatta ttttttatgt tgggttcaag
     8101 aaacgaagaa aaaggatttg taaatggtct tcataatata aactttaatt ttgatgaaaa
     8161 gatatgtgta aatgcattga atgtttattc aaaattatta aaatacaaag aagcaataaa
     8221 ttaggaagat aaattataaa gattaaaaag cataaatacc taaatttaaa aaaagtgtat
     8281 taataaatac cccttacata tttgtataaa tttattttaa ttgacaaaaa tacaaataag
     8341 aaggggtgat ttttttgaaa gaaaaagtac aaagagaaaa gaagataact aatggcaaga
     8401 atactaaaat attgacaaat gatgatataa tgaaatatga gatagcctca gaactaggtc
     8461 ttatggataa agttggtgaa cgtggatggg caggacttac agctaaggaa gcaggaagaa
     8521 ttggaggtat gcttacatca cgcaaaaaga ggcttaaaaa gattgtggag gagcagaata
     8581 aaaaggatga gtcaatatag tgattttgcg tttgtatatg atgaacttat gaatgaagtt
     8641 gattataatg gttgggttaa atatatagaa gatataatag atagtgaagg tgtaaaagta
     8701 aaaaatatat tggaattggc atgtggcacg ggtaatctta caattccatt gactaaaaaa
     8761 aattacgata tagctggtat agatatatct gatgagatgc ttagtgtagc aagagaaaag
     8821 tcagaaaaag aaggagtaga attagtttta cttcaacaag atatatcaga gcttgatttt
     8881 gaaatatctg atttggactg tgtgctttgt gcttgtgatg gatttaatta tataacttat
     8941 gatgatgatt tggaaaatgt attttcaaag acatatgaat tattaaagaa agatggaata
     9001 ttcatatttg atataagttc tttttataaa ttagccaaca tattgggaaa taacatgtat
     9061 ggagaaaata gagaagatat agcgtatatg tggcaaaatt actttgatga agaagagaat
     9121 ctaattgaaa tggaacttac attctttatt aaagatgaat gtggaaagtt tgaaagattt
     9181 gaagagatac accaacaaag agcatatact gaagatgaga tagttgatat gcttgaaaaa
     9241 tctggattta aggatataaa atattatggg gattttacat ttgacaaacc tagtgaagaa
     9301 agtcaaagaa tcttctttgt atgtaaaaaa taag
//
LOCUS       DAFKMX010000157         9325 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFKMX010000157 DAFKMX010000000
VERSION     DAFKMX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.157"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            49..627
                     /locus_tag="LZB08_002946"
     CDS             49..627
                     /locus_tag="LZB08_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7316430.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            666..1244
                     /locus_tag="LZB08_002947"
     CDS             666..1244
                     /locus_tag="LZB08_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7316431.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1330..1959
                     /locus_tag="LZB08_002948"
     CDS             1330..1959
                     /locus_tag="LZB08_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBM7316432.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKNLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            1949..4708
                     /locus_tag="LZB08_002949"
     CDS             1949..4708
                     /locus_tag="LZB08_002949"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBM7316433.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4795..6357
                     /locus_tag="LZB08_002950"
     CDS             4795..6357
                     /locus_tag="LZB08_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBM7316434.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6358..7494
                     /locus_tag="LZB08_002951"
     CDS             6358..7494
                     /locus_tag="LZB08_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBM7316435.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7558..8634
                     /locus_tag="LZB08_002952"
     CDS             7558..8634
                     /locus_tag="LZB08_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBM7316436.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8612..>9325
                     /locus_tag="LZB08_002953"
     CDS             8612..>9325
                     /locus_tag="LZB08_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBM7316437.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTCLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISS"
BASE COUNT         3678 a          970 c         1790 g         2887 t
ORIGIN      
        1 taattagcaa gatttgttta gctaaaatta acaagggagc gataaattat gggtataaca
       61 ttatcaaaag gccaaaaggt tgatttaact aaaggaaatc caggtcttaa aaatatttta
      121 gtaggtttag gatgggatac taacaagtat gatggtggat ttgactttga tttagataca
      181 gcagcatttt taacaggagc aagtggaagt gttactaatg atggagattt tgtattttac
      241 aacaatttaa aacatacatc tggagcagtt gagcatctag gagataacag aactggcgaa
      301 ggtgatggag atgatgaaca aatagttgtt gatttatcta agattcctgg agaaataagc
      361 aagatttctt ttactgttac aattcatgat gcaacagaaa gaagacaaaa ttttggacaa
      421 gtaagtaatt cttatataag aatagtagat aaagacacaa atgaagagtt aataaagtat
      481 gaattaggag aagattttag tatagaaaca gctatagtag tagctgaaat atataagcac
      541 aatggagaat ggaagtttaa tgcactaggt tctggctttg aaggtggtct tgctgcatta
      601 tgtggaaact ttggaataaa cttataattt caactataac aataaactaa agggggatat
      661 tttccatggc tatagtatta aaaaaaggac aaaaaatcga tttaactaag ggaaatccag
      721 gtcttaaaaa tataaaactt ggactaggat gggatacaaa ttcatttgat agtggctatg
      781 attatgattt agatgtaagt gttttcatgg ttggagaatc tcaaagagtt gaaaaagatg
      841 aagattttat attctacaat aacttaaaac atccatctgg agcagttgaa cacttaggtg
      901 ataatagaac tggtgaaggt gatggagatg atgaggaaat attagtgaat ttagagacaa
      961 tgccaaagca tatacaaaag atagctgtaa cagttacaat atatgaggct gctgaaagaa
     1021 gacaaaattt tggacaagta agcaactcat atataagagt attaaattct gaaaatgatg
     1081 aagaaatatt aagatatgac ttaggtgaag aatttagtat agaaactgca attacagtat
     1141 gtgagatata taagtataat ggagaatgga agtttagtgc agttggagca ggatttgaag
     1201 gtggcttaga agcactttgt agaaactatg gcttaaatgt ttaatttaag caatataata
     1261 tattaaatat aaataaccta tacaaataat gtataggtta tattatataa aattttgtgg
     1321 aggtttagta tgtcgattaa tttatcaaaa ggtgataaaa tagatttaaa aaaatctaac
     1381 ccaggactga gtaacatact tgtaggtcta ggatgggacc ctgtgcaaca atctggcgga
     1441 ggttttttta aatcgttatt tggtggagga caagctgata tagattgtga tgcatctgta
     1501 tttatgctta atcaagaagg taaattaagt ggaataaaaa atttaattta ttttggaaat
     1561 ctaaaaagtg cctgtaaatc agttttgcat acaggagaca atctaactgg tgagggtaca
     1621 ggtgatgatg aacaaatcct agttaatttg gataaagttc catcaaatat tcataaatta
     1681 ttgtttgtag taaatatata caattgtgta gatagaaaac aacactttgg aatgatagaa
     1741 aatgcctata taagagttga agaccaaggt aataagaaag agatagcaaa atacaatcta
     1801 tctgataatt attctgaaaa aactactctt atagtaggag ctatatatag aaaagatgga
     1861 agttggcaat ttaaagcaat tggtgaagga acaaaagatg cgggtcttaa agaagtaatg
     1921 caaaatttag acaggattga gtgtgcatat ggaatttaat aatgatatca aatattataa
     1981 tgtagataaa gaagaagtta caaaagactt atctgttaat cctgaaaaag gtttatcaga
     2041 atcagaagtc aagactagaa gagaaaaata tggtttgaat gaatttactc caaaagaaga
     2101 gggtagtttt tgggatgatt taaaggaaag tttaagtgaa ccaatgatag ttatcttgat
     2161 tattgcagca gtagtaagtg cagtaattgg ggaaactcat gatgcaatag ggattgtagg
     2221 tgctatagca ataggtatag caataggtat gattacagaa ggtcgttcta agaaagcagc
     2281 agaggctctt tctaagttga ctgaaaacat agaagtaaaa gtattgagag atggggaagt
     2341 acatcaaatt tctaagagtg aattggtacc aggtgatata gtatatattg aaactggtga
     2401 catggtacct gctgatggta gacttataga aagtataaac cttaaaatta gagaagatat
     2461 gttaacagga gaatctgatg acgtatctaa aaagtgtgat gtagtagttt ctatggaaga
     2521 gatagaatct aaaggagcag tagtagtaca ggaaccaata cctgcaaaac agataaatat
     2581 ggtgtttggt ggtacacttg tagcttatgg tagaggagct ctagttgtaa cttcaattgg
     2641 agataatagt gaaatgggta aaattgctca aaacttatca gaaacagatg agcaaactcc
     2701 acttcagata aaacttggaa atttaggtgg tttgatagcc aaagtttcta gtgcagtagc
     2761 aggattacta tttatattta tggttttcca aatgataagt aagaatgtat taaatgtaga
     2821 tatgtctgga atattaccgt ttttagaatc aatagaccca gttaaaacag catttacagt
     2881 atgtgttgcg ttgatagttg cagcagtgcc agaaggtctt ccaactatga taaatatgac
     2941 acttgcaata acaatgcaaa aaatggctaa aataaatgca ttggttacca aaaaagaagc
     3001 ttgtgaaacg ataggttctg tttctgtaat atgttcagat aaaactggta cattaactga
     3061 aaatagaatg actgtagaag ttgcatatgt tgatggcaga tatattgaaa gttcagaaga
     3121 ggaaataaat agttattttg aagaaaactg tatgataaac tctacagctg atgtagagca
     3181 caatgatggt gatattaagt atttaggaag tgctactgaa tgtgcattgc ttttatatta
     3241 caaaaacgta gattatagac aagttagaaa aaattctaat atagtagctc aaaatccttt
     3301 tacttcagat tcaaagagaa tgacttctgt tataggtcaa gataatcatc atgtactatt
     3361 atcaaaagga gcaccagaag tcttacttga attatgttca catattcaaa gaggaaaaga
     3421 catagttcca ttaactgaaa atataaaaca tgagatttta gaagaaataa agaaattgca
     3481 aataaaatca atgagaactt tagggtttgc atataaagaa atctcacaag ctgaagaaga
     3541 agcagcagta actgctgaaa gtgatgctat gaatgtaagt gcaatggaaa acaatttagt
     3601 atttagtggt tttgttggta taagagaccc acttagaaaa gatgttatag agtctgtaaa
     3661 tacagcaaat aaagctggtg tttctgtaaa aatgcttaca ggagataata taaatacggc
     3721 aagagctata ggtgaagagt taggcctatt aaaaaataat atgagagtgg ttgaagcttc
     3781 atatatagat actttagatg atgaagaatt aaaacaagag atacaaagta tttcaatcgt
     3841 ggcaagaagt aaaccagata gtaaaatgag aatagtttca gctctgcaaa agagcggaga
     3901 tgttgttgca gtaacaggtg atggtataaa tgatgctcca gcactttcaa aagcagatgt
     3961 tggtatagca atgggtatat ctggtacaga ggtatctaaa aacgcagcag acataatttt
     4021 aactgatgat agttttagta ccattgtaaa aggtattaaa tggggtagag gaatttatga
     4081 taacttccaa agattcgttc aattccaatt aactgtaaat gtaattgcat ttttagtggc
     4141 tataatatct caagttatgg gacaagagat gccatttact acaatacaat tattatgggt
     4201 aaacatcatt atggatggac caccagcact agcattaggt ttagagccag ttagggacca
     4261 tgtgttaaat agaaaacctg ttgatagaaa agccaatata atttctaagt caatggtgta
     4321 tacaatagta ttaaatgcat tttatataac agcaatacta atgttacaat ctacatttaa
     4381 tttcttagga gttgatgaag cgcataaggg aacagtaatg tttgcgttat ttgcttttag
     4441 tgcactgttt aatgcattta actgtagaga gtttaatgca gatagtatct tccctaattt
     4501 tacacataac aaattagcac tacaaataat agtgttgaca ggaattgcac aagtaatatt
     4561 tactcagcta ttccaagatt tctttaactc tgtagcaatg gattttatga catgggtaaa
     4621 agttttatgt gtagcagcaa gtgtaattgt ggttaatgaa gttgtaaaat gtataataag
     4681 actggtaaaa ccaaaaaaga ctgcttaaaa caaatttttt agaagaaaac agactgactc
     4741 aaaatatgaa ttttgggtca gttctgaata aacatttaaa ttgggaggat agttatgggt
     4801 gactcaaagg tatttgaaaa aatattctcc agccaatcag atagaggaaa ttacactcct
     4861 tcaaagggat atctaagtta ttttatttcg tatattggtt tagaagatga agttttgtac
     4921 aatctcgaga tatttaaaac aaaacaaaat attgatagca aaaaagatat tgctttattt
     4981 actgatgtta tagcaaatcc tagtgatttt gatattataa attattttaa aagtggactt
     5041 caaaaatata gaactagcat ggaagatgta gatataaata ttttgggatt tgaagagatt
     5101 gattataaaa taaagcaagc aatggataga gtgttaaaag aagaggaaaa agaatttact
     5161 aatgatagag ttaaacaaaa ttttatagtg aaaataatgg catggataaa aatttatatc
     5221 ggtgctttag atataaataa aaatgaagct ccaaaagtaa tattttatgg agatataaaa
     5281 aaacatgaag tttatttact cttaatatta tacttggcag gttttgatgt tttatatctt
     5341 aatccaaatt caaagagtaa tatagatatt ttaaaatcag aaagatacaa tatagagttt
     5401 gaagaagcca acataataga agaaaaaatt tcttttgaag aaagggttat tttaggggaa
     5461 aaaatagata aaagttctgt aaaaaaagct tttactgttg gggcagaagc atcaaagcgt
     5521 ataagtgagg aattgttaaa tgatgcagga tttataaaac cttggcaact ccaagataga
     5581 aaaataaaaa atctacttct aagttcaact gttgatgaaa tatcaatata ctggaatcaa
     5641 ccacttaaat taagaccagg atttaagttt aatgatgcta ttgtagaagc tccaaatttt
     5701 ctttctaaga ttaatggaat atataacgat aagaatgaat atattaagtt tttagattta
     5761 ttaagagatt cggaaagttc aacatttatt gaatttaatg gagatgtaga tagattttca
     5821 aaagctttta caagagaagc ttttagttta tcctttttat tggattcaaa aggtgcaata
     5881 gataaaaact ctgttttaaa taataaagat tattccatat caacactagc attgaatcaa
     5941 caaattatga ttttagagaa agtggaagag ttgttagaag gaagtatgtt tttgaatggt
     6001 ttaagtggag aagacaagat aaaaggtcta tttacagtac ttcacatgga taagaaattt
     6061 gttcatatga tgaacaattt cgattactca cttataaacc caaaattgat tatatacatg
     6121 tacaagagta ttgtatttga taaagaaata gtttttctta tgcttttact tagtaagatt
     6181 ggatttgata ttatcatatt atgtccagga ggagaaaaca atatagagaa tgttataaat
     6241 aaccaattga ttgatgtaca cagattggat aaaatggtat atgaccttaa attaaattcg
     6301 cttgaaaatg atataccttt acttaaaaaa atttttggaa agaggagacg attttagatg
     6361 gggataaata taaatagttt ttcaaaagat aagaactctg tagaggttac tgagaatttg
     6421 cctacagcag ttcaagaaga aaacttcgat ataatggaat atacaaataa taaaaaaaat
     6481 gaactgagaa agtctaagga agttgaggca ttgacatctc ttatagaagt agaaaatcca
     6541 gatacgattt tacagtttgg taggaaagca tctgaaggag tagcaagagt ttctgatagc
     6601 cttttaaata caataaaatt aaatagaaat gaagaaaatt ctaaaatgct ggttcattta
     6661 actaaaataa tggacaagtt tgatttagac gattttcaag aaactaagga accaaatttc
     6721 gttcaaaaac ttttcaaaaa agctaataat gccattgaaa tgatgtttca aaagtatgaa
     6781 acattgggag gagaagttga gaaaattcaa attgagcttg aacaatacga aagagatata
     6841 gcactttcta ataagcaaat aggtgctatg ttaaatgaaa actttgagtt ttacaatgaa
     6901 cttcaaaaat atattgttgc tggagaaatg gcaatagaag aaatggataa tgaaatacta
     6961 cctttcttta aacaaaaatc agagactagt ggagaccaaa tggatgttgt caattatcaa
     7021 gaactattaa aagtttatga tatgttaaat caaagagtct atgatttaag gatagcagaa
     7081 aatatagcta tccaaacaat acctatgctt agaggtatgc aacataataa ttatggttta
     7141 attagaaaaa taaatagtgc atttgtcgta actttaccag tatttaaaca atgtttatct
     7201 caagctatat tacttaaaaa gcaagaatta caagcaaaat ctttaaaagc acttgatgac
     7261 aaaactaatg aattattgct tagaaatgca caaaatgtaa gtactcaaag tgcacaaata
     7321 gctagaatgg ctggaacaag ttctgttcaa atagaaacat tggaaaaaat gtacaatact
     7381 atcaaatctg gtatagatga aactatgaga atagaagaaa acaatagagc tttaattaag
     7441 gataatacaa agagattgga agaattaaat acgactataa tttataataa gtagtattat
     7501 attaagggct aatttgtata aagttggccc tttctatgta tatgaggagg ctagacattg
     7561 aaatatttcg attatatctg caaagatgat ttggagagaa tttttttgaa ggagccagaa
     7621 gattttagtg ctaagactga aaaagatgtg ttgaaatatg cactgggcgc ttttttatac
     7681 gttccagcta ctcaatataa tatgatttat aagagtatta taggggatgt taagggagtt
     7741 agaccattag ctatttgttt agaagatgct gttggagtaa atggagagtt agaggctata
     7801 gagaatctta gattaatttt aaaaaatatt agcaatgaaa gtataactaa taaagatgga
     7861 ataccactaa tttttgtaag aataaaagat gtggagcaac tgttgagaat taaagagatt
     7921 ataattaaaa atagtcattt tataacagga atattaattc ctaaagcaaa ttcggagtta
     7981 attgaaaatt gtatagaagc tttagattct atgaatcttc aagatatgta tgttattccg
     8041 atagtagaga caaaagaatt tatatataat gaaaaaaaag aacttagttt tactaatttg
     8101 tacaatgcta ttttaagaca taagtcaaga atattaagca ttagagtagg cataactgat
     8161 atattaggga tgtatggtat aaggagagat aaaaactttt ctatatataa taatttgatt
     8221 tgttcttcat ttatattaga tataatcaca tatcttcaaa gaccagagtt agatatacct
     8281 ataagtggtg gagtatcaga attttttgat atgacgaata aagatataag aaataagtat
     8341 atagaagaaa tattattgga taaatatcat ggattaattg gaaagaccgt aattcatcca
     8401 atgcaaattc aaattgtaca agcgctatct acagtatcat atgaagattt tactgatgct
     8461 ttggatatac tggatagtac agacagtaag tatggtgtga gcaagggtgt attaggagag
     8521 agaatgaatg aaacaaatcc acatttttta tgggctaaaa aaacattgat tctatctaaa
     8581 atatatggtg tattaaataa aggagtagac tatgaagaat tgcttaaatt ttaattgtga
     8641 gattgacatt attgataatc cattgaattt agaactaaat gattttttag acgtaggaat
     8701 aagaaataat agcaaaagac gttttttgtt tatctcaaag aaactcggta aacatttagc
     8761 atgtaagcca agtgagatgg ataatttggg aaaattaatg gctacaattt ataatgaaaa
     8821 aaataaggaa gaatcaaatg gtactgttat agcttttgct gaaactggta cagctgttgg
     8881 gcatagtttt tttgattact tatgtggaga ttatgaattt attcatacta ctagagagca
     8941 gtttgacgag ttagaaaatc tggaattttt agaggaacat tcccatgcaa cagaccagaa
     9001 tttatacttt ggtatgctta acaactttaa atttggagat gagataatat tggttgatga
     9061 tgagattacc acagccaata catgcatgaa tataattagg aaaattcaaa gcatttatcc
     9121 taaaaagaaa tacactatat gctcaatact caattgggta agtgatgaaa atttaagtaa
     9181 agttacttgt ttagaaagag agctagattg taagatagat tttgtatact tatttaaagg
     9241 aaactttaaa ttttatatag atgaagatat tatttcaaat tacaacgata attatagtat
     9301 tactaaatta gagactattt caagt
//
LOCUS       DAFKMX010000158         9192 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFKMX010000158 DAFKMX010000000
VERSION     DAFKMX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.159"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            393..1598
                     /locus_tag="LZB08_002954"
     CDS             393..1598
                     /locus_tag="LZB08_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBM7316438.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="LZB08_002955"
     CDS             complement(1669..1890)
                     /locus_tag="LZB08_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316439.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="LZB08_002956"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="LZB08_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBM7316440.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="LZB08_002957"
     CDS             complement(2643..2993)
                     /locus_tag="LZB08_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBM7316441.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="LZB08_002958"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="LZB08_002958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBM7316442.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="LZB08_002959"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LZB08_002959"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="LZB08_002960"
     tRNA            complement(5068..5159)
                     /locus_tag="LZB08_002960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="LZB08_002961"
     CDS             complement(5160..5615)
                     /locus_tag="LZB08_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBM7316443.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="LZB08_002962"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="LZB08_002962"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBM7316444.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="LZB08_002963"
     CDS             complement(7666..8547)
                     /locus_tag="LZB08_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBM7316445.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="LZB08_002964"
     CDS             complement(8559..9068)
                     /locus_tag="LZB08_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBM7316446.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2943 a         1600 c          974 g         3675 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttaattttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt gtcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa gcttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaagatattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtcgcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatgatact gccctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagtg
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcttca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctacaataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 tgtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctacatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tatcgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta agaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaagtata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta caatgacatc ttttataata ccacctaaat ctacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaaa ga
//
LOCUS       DAFKMX010000159         9135 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFKMX010000159 DAFKMX010000000
VERSION     DAFKMX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.160"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            166..510
                     /locus_tag="LZB08_002965"
     CDS             166..510
                     /locus_tag="LZB08_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316447.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            complement(842..2218)
                     /locus_tag="LZB08_002966"
     CDS             complement(842..2218)
                     /locus_tag="LZB08_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBM7316448.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(2317..3339)
                     /locus_tag="LZB08_002967"
     CDS             complement(2317..3339)
                     /locus_tag="LZB08_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBM7316449.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            complement(3350..4144)
                     /locus_tag="LZB08_002968"
     CDS             complement(3350..4144)
                     /locus_tag="LZB08_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBM7316450.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            complement(4155..5297)
                     /locus_tag="LZB08_002969"
     CDS             complement(4155..5297)
                     /locus_tag="LZB08_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBM7316451.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            complement(5337..6569)
                     /locus_tag="LZB08_002970"
     CDS             complement(5337..6569)
                     /locus_tag="LZB08_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBM7316452.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEYTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            complement(6706..8046)
                     /locus_tag="LZB08_002971"
     CDS             complement(6706..8046)
                     /locus_tag="LZB08_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBM7316453.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            complement(8141..8935)
                     /locus_tag="LZB08_002972"
     CDS             complement(8141..8935)
                     /locus_tag="LZB08_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBM7316454.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
BASE COUNT         2814 a         1858 c         1083 g         3380 t
ORIGIN      
        1 ttcacaggat attattttta caattagttt tattttcgtg taatttatat atgttgtaat
       61 taataaaact aaatatgatt taaataattc cttgggataa atttaataaa acttagattc
      121 cttatctaag cttaaaatac taaaattatt gggaggatat aaataatgga ccaaattaag
      181 cgtttacatg aattgcaaca aaaatcatat ttaactaaag aagaatttga ggaattaaaa
      241 aataacacat atattgaaag atttgaatta agagatgagt gcgataactc ttatatttat
      301 acattttaca ctaacgataa atgcaatgat ataaaattta taaaatcaca atttatcgtt
      361 acacttataa aaattggttt cctatgtgag tgtggtggtg tatttagaca aaagaaaata
      421 gttgatgaat atgattacgg atacaatgct atatataagt gtgattcttg tggcaaagaa
      481 cttgataaag aagtagaaaa ttatgactaa ataacaaaaa agctattcaa aatatttttt
      541 ataaatgcta gataaaattt actctaaatt taaactaaac tatcccctct tagtagacag
      601 ttaaaatata aaaaactgtc cataactaag agggttttgt tatatcctaa attaaacata
      661 ataatcttag atatcaaaag atactgaaat atagatagta attataaata taataaaaaa
      721 gtactaatga ttgacattag tacttcacat ctacttttac acgttttaac tgcataaaac
      781 agtttataca gttcgctttg aataagggcg cttttttagc attgccttaa ttcaatttat
      841 cttaattatt taattcaatt tcatactttt ttattttctt atataaaaga ctcttgtgta
      901 ttcccataac ctctgccgcc ttagaacatt tccaattata cttccttaac acatcaataa
      961 ttaagctctt ttcataattc tcaaccattt catgaattga acaattgcta tccacattac
     1021 attgagctac tctgctgata ttatccatct tagatggaag gtcacacatc ataatgacca
     1081 tatcatcact tacagcatag gcacttttta ttacattttg tagttctctt atatttcccg
     1141 gccaagtata gttaataaaa caatttttaa ccttatcact taaaaccttc aattccttgt
     1201 attcaacatt taatttgttt aatatataat ttgctagcaa taaaatatca tcttgcctgt
     1261 ccttcaattc catcatatga atgtttatta catttaatct atagtatagg tcttctctaa
     1321 ataatccctc ttcaaccatt ttacttaaat tctttcttgt ggcagagatt actcttacat
     1381 ctatctttat agttttaaga cctcccactg gttctatctc actttcctga agaactctta
     1441 aaagttttgc ttgcatggtc aaaggcatgt cacctatctc gtctaaaaag attgtaccat
     1501 tattcgcaac taaaaattta ccttttttac cacccttttt tgctcctgta aaagctcctt
     1561 cgtcatagcc aaatagctct gactctaata attcttctgg tattgcagcg caatttatac
     1621 taaccatagg tttatctgac ctgctgctat tattgtgaat tgctctagca aaaacttctt
     1681 tacctgtgcc tgtttcaccc gttaaaagta ctggaaagtt tgttttagaa gctttcacac
     1741 ctgttttctt aagttctatg aagtctctat tttcacctat gagcttgtca aaaccacagt
     1801 tatccttaaa ctgctcttta taatattgga gctccatata ttctgacatt aactttttag
     1861 ctacatcaaa tgattgcaat ctaaatttaa cttgagctac tcctgcaact acttctccat
     1921 tgtcattttc cacataagac ctacttacta ttacactttt ttcttttgta tttccacctg
     1981 aataatgatg tatagctcct tcttcacaat acttattttt cataacatct agcatttttg
     2041 tatttggtat aatactttgt atattttgtc ctattatatc ttttctttct tttcctaaaa
     2101 agtcacaata tttatcatta atatctatta ttactccact tgtatttaca acaataaaac
     2161 catcatcagt catagataat actcttgcaa atatatcctt ataccactgc tctttcattg
     2221 tttcatctcc ccactataac cttcagcaaa aataataagt cttacaatac aatattataa
     2281 gacttatcat tctacatatt caatgttttc taaaagttac ctatttaatg attctatcat
     2341 ttcaggtata atttcatata aatctcctac tattccatag tcacatatac taaaaatagg
     2401 ggcttttgca tctttattta tagcaactat atatttagaa tcactcatac ctgctaaatg
     2461 ttgtattgct ccagatattc cacatgcaat gtatagttct ggtctaacag tagttcctgt
     2521 ttgtcccact tgatgagaat gttctatcca ccctgaatct actgctgctc ttgaagcacc
     2581 aatctctgcc cccaatttat cagccaattc ttttataagc tcaaatcctt ctgctctttt
     2641 taatcctctt ccccctgata ctattattct agcatcagtt agatttacag atttcttttc
     2701 ttgtggaagt atctctacta atctagttct aatcattttt tcagtaagtt ctggagtaac
     2761 aacttctaaa attccagtct ctgattcgtt tcttacagct tttgccataa ctcctggacg
     2821 aactgttgac atctgtggtc tattttttgg acaaacaata gtggccatta aatttccacc
     2881 aaatgctggt cttgtttgta ataacttatt atctgttgaa tctatttcaa gttttgtaca
     2941 gtcagcagtt agacctgttc caaccttacc tgcaattcta ggcgctatat ctcttcctat
     3001 agaagtagca ccaaccaata caacctctgg cttttttctt tcgattaaat ttgcaattga
     3061 aattgcatat ccatcagtag tgaaatcttt aagaagaggg tcattgatgt aatatacttt
     3121 atcaacacta tagtgaagta attccttcac ttctttctct acttcatatc cagctatcac
     3181 aactgcaagc tcttttccta attggtcagc aagctttctt ccttcaccaa taagttctat
     3241 cgtaacagga ttaattttac cttccctttg ctctcctatt acccaaacac cattataatc
     3301 atttaaattt ataccttgat taacttttgc tctcatcatc ttaacactcc tatactagtt
     3361 tcaaatcgaa tagtatttct attagtttct cagctttttc ttttttattt ataccttcta
     3421 ttatttcatt atgcttgtct ttaactggta caaaagactt ataaacgttt gtaggtgaac
     3481 cctttaatcc aatttgagtt gtatcaacat ctaaatctgc aagtgttaat acagtaatct
     3541 ttgcatctcc attattataa gcttctaaga ttcctttgac acttggatat cttggattgt
     3601 ttaactcttt tatagcagtt aataaaactg gcatctttgc ttctattaaa taatcccctg
     3661 tctctgtata acgagttact agtaatttgt catcttgaac ctttacttct ttcgcataag
     3721 taacttgtgg tatccctaaa aattctgcaa cctctggtcc aacctgagct gtatctccat
     3781 ctatagcttg tcttccgcag aatattacat catactttcc aaccttctct atagcagcag
     3841 caataattgt agatgtagcc caagtatctg aaccaccaaa agctctatct gagattaaat
     3901 aagcttcatc agctcccatt gccaaagctt cttttaaagc acttttagct tgaggaggtc
     3961 ccatgcttat aaccttaatc actgctccta gttcatcttt catttttaga gcttcttcta
     4021 aagcatttct atcatctgga ttgattatac ttggtacacc atctcttatt agagtacctg
     4081 tctctttatt tattttaact tcattagtat ctggaacttg ctttaagcaa acaactatat
     4141 tcattctttt cctcctatcc tagtactgct cttgaaataa caactttgtg tatttctgaa
     4201 gtaccttcgt aaatttctgt tattttggca tctctgtaca ttctctctaa tggataatcc
     4261 ttcatatatc catatccacc atgaatttga agggctaaat ttgtaacctc tcttgcagta
     4321 cgtgacgcat tgtattttgc catagcagct tctttagtgt gtttttctcc tctagctttc
     4381 aaatctgcag cataatatac taataatttt gctgattcca ccattgtcgc catatctgct
     4441 atataccatt gaataccttg caaagctgaa attggcttgc caaactggac tctttcttta
     4501 gaatatttga tactttcttc taaagcccct tcagcaattc ctacagcttg agctccaact
     4561 ccaattcttg ctccatctaa acaagtcata gctatattaa atcctttgcc ttcttttcct
     4621 aataagttag actttggaac tctacagtta tcaaatatta actctgcagt ttctgaacca
     4681 tgaataccca ttttttcttc aatttttcct atcgaaaaac ctggagtacc tttgtcgaca
     4741 atgatagcag acattccctt tattcccttt gatgggtctg tcagtgcaaa tattattaat
     4801 gattctgctt gacctccacc agaaataaaa cacttagttc catttaaaac atattcatca
     4861 gtttcttcat caataattgc agttgttgca gctctcgcag catctgaacc agcattaggt
     4921 tcggttaagg caaaagcacc tacacaccca ccttcagcca tcattggaag gtattttttc
     4981 ttttgttcct cagtaccaaa tttcaaaata gcttgagcat atattgtgtg tattgataat
     5041 attgctgcag atgctgcaca ttttttggca atctcagtaa caacaattac cttttctata
     5101 tctgccccac cagaaccact atactctaca ggtgttccaa ttccattaaa tcctatttta
     5161 gccattttgc taaaaagctc ccttggaaaa atacctgttt tgtctgtttc agcagctatt
     5221 ggtgctattt cattctctgc aaattctttt gctactcttt gaagcattaa ttgtgcttct
     5281 gttaatctaa aatccataaa attccctcct atttattaac atacttcaca tgtttattaa
     5341 catatttcac atgcttcttt attcattgtt acaattccag attcaattag agcttctata
     5401 tcagaatcac tgtatccaag ttctctcaat atctcatctg aatgttctcc tataagtgga
     5461 gcatcacaag tcatgttaac ttcaatattt ccaaatttaa ctggagtaga cgcaagcatt
     5521 gtcttattcc catttttact tgtatattca tatatatagt tattagcaat agcctgttca
     5581 tcagtcacta catcattaac atgttgaatt ttttcatgag ctatatcagc agcagtaagt
     5641 ttttcaacca tctcattttg agtaaacttt gcaaattcac catccaaaat actaataagt
     5701 tctgtggcat ttttcttact ttctactgaa gatgcaaatt tctcttcttt tattaagtct
     5761 tctcttccaa ataatctaca aagtgcctca aaatatcttt catgctctaa aatacttaag
     5821 aatatccact ttccatcctt acaacgataa gaatttatta ctggtgaaat agcatttttt
     5881 ctagtttttg gatactcatc actaaactgt gtagatgcta ataaagatga tgcattccat
     5941 attgcttgcc caaataaaga tgccattact ttttgtcctt tgccagtctt agcttgctgg
     6001 tacaaagctg cacatattcc tccagataaa ctacaagaag ttgttgcatc tccaaaggct
     6061 ataggtggat ttattggact tgtatctttt tctgctatat caagcatagc cccacttctt
     6121 gcccaaaagg caactgtatc aaatcctgca ttatccttag caggtccaaa atcgccaaaa
     6181 ccatttatct gaccccagat aaggtgtgga tgctttttac ttaatgattc ataatcaaga
     6241 cctagtcttt ttaatgctcc tgttctataa cttgaaacaa atacgtttgc ttcgcttaaa
     6301 agcttatcca taatttcaat tccagtttca ctcttaagat ttatagacaa acctctttta
     6361 tttgaattat atgtgctgta aaaaggatta ttataatcat ctattggcat tgtcattgtt
     6421 gcccctgtct ttcttcctgg gtctccaaag cttggctcta ccttaataac gtcagcaccc
     6481 cagtcagcta atattttaga agtccctgga cctgctacaa aatacgttag gtcaataacc
     6541 ttaactccct ctaatggttt atttaacact ttcaattcct ccttatatgg tcaatttgtt
     6601 attttaaata catatttaca gtattaaaac atgaaattta ttgtatatat tttgaaaatt
     6661 gattttataa catcaaaata taatttatac cttttatctt ttctattatg gcaatcccgg
     6721 gaacattgta aacagtagga ctgctactat ggttccaata aacggagtta aaacagttac
     6781 ccagaatatt ggcatatagg catccttatg tgtttcttta cacagaccat ttgttacagt
     6841 aacaatatat ccattgtgtg gaagtgaatc taatgctgct gatgaaattg ccatagtcct
     6901 agttaatgct gcagcggaaa cacccttggc taaaaatact ggtccaagaa gtggagctgc
     6961 aattcctagt ccacctgaag ctgacccaca aatacctgca ataactgtag ttccaactgc
     7021 agctcctgca ataggaggac caggaatatt agtcatggca ttaactatga agtcaaaacc
     7081 aactgctgat tttactactg aaccaaatgc aactacagca caagtattac atattgctac
     7141 tactgtagtt ttacatgcat caccaacatg tccaactatt ttagtattat cttgatattt
     7201 atttaacaat aaatatacta ataaagctcc aataaatact cccacctcaa ttggtactat
     7261 cgcttttcca ttaactttta cattaattat taaaattgac actatcaatg gaattaaggc
     7321 aattacacca ttgggaccat tttctctctt agaattagtt gcttctgctt catcaaatat
     7381 atctgattca tgtgctataa aatgaccacc attggctttt tcttttgaaa ccattttgta
     7441 caaaattgta cttccaacta taaacattac tgcacatgat ataaaaccat ttactgctcc
     7501 agccataata tctgtcccta atgttgatgc tggaacaata ttctgaattt gtggtgcccc
     7561 tggtgcaacc atagcaaaag ttgagcatcc aaaagtcaaa gctgctggta taaatcttcg
     7621 tggcaaatca gcttctttaa aaatttctaa agctatcgga aacactgcaa aacttacaac
     7681 gaaaacactt actccaccat aggctaatat tccacaagct attggaatag aaagtagtgc
     7741 tttatccttc ccaatccatc ttacaatcat cttagcaata gattttgctc caccagtaat
     7801 ttccattatt tttcccatta atgtccctgt taggaaaata aagaaatttg cctgaagaaa
     7861 tccaacaaac cctgtcatat aatccacttt aagtgcatca tacacattta gacttcctgt
     7921 tatagcaact aatgatgaac atacaattgc tataataaaa atatttattc ccttcataac
     7981 cattacaact agaagagata gagctatgat aatccctact aaacttataa ttactcccgt
     8041 attcataaat taactccttt taaaattaaa ctatatgtat tttttcacga aatttagatt
     8101 ttgaatatat tctacattgt tccaatttta gttataatag ctacttattt tcaaaaactg
     8161 gctttctctt ttctataaat gcattcattc cttcttgctt atctcttgta gcaaaagtta
     8221 aacctgcaat atctttctct agttcaagag cattctttaa atccatatct atccctttgt
     8281 taataacaac ttttgcatat cttattgcta ttggcgcttt ttcaactatt tggttcatca
     8341 tcttcataga ctcttctaaa agtaaatcac ttggaaccac tttattaata agcccaatag
     8401 atttagcttc ttctgcattt acttgtctcc ccgtaaaaat taactcttta gctattccag
     8461 ttccaacaag tcttgataat ctttgagttc caccaaaaca tggaataact cctaaattaa
     8521 cttcaggttg tccaaatata gccttttctg atgcaattct aatatcacaa ctcatagcaa
     8581 gttcacatcc accacctaat gcatatccat taactgctgc tattactgtt ttctcaagag
     8641 cttcaatttt atcaaaaatt ccctgagcat aacctgcata ttttctgcct tcctcacttt
     8701 tatagttttg catctggcgt atatcagcac cggctacaaa aactttacct tctccagtaa
     8761 taataactcc taatattgcc tcattttctg atactacatc aataacttgt gatagttccc
     8821 taagagtgtc actattaaga gcatttaaag ctttaggtct atttatagta ataacacaga
     8881 ctttgtcctt aacctctacc ttaagattgt tcaaattttc cattacagat tccatcatat
     8941 tccccccatt atacattgtt attttttcta catttcttgt taatataaaa tagcaagtac
     9001 tatgccaatc accattgaag ttctttattt ttagtaaaaa attgttattt ttaataaaat
     9061 aaacgaaata tacataaatt tcactaaatt tttaataatt ttcaacttta aaattgatta
     9121 aattgtaatt cacag
//
LOCUS       DAFKMX010000160         9134 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFKMX010000160 DAFKMX010000000
VERSION     DAFKMX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.161"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..435
                     /locus_tag="LZB08_002973"
     CDS             <1..435
                     /locus_tag="LZB08_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBM7316455.1"
                     /translation="YNNIILYDYLYNKGEYSYIYENKREESEIIKYYNKTDLLYLKNV
                     VSNIVPSRDEFITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILNVFRELNLLDFN
                     LNYEKNSVLIRILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            448..960
                     /locus_tag="LZB08_002974"
     CDS             448..960
                     /locus_tag="LZB08_002974"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBM7316456.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            1034..3241
                     /locus_tag="LZB08_002975"
     CDS             1034..3241
                     /locus_tag="LZB08_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBM7316457.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3266..3715
                     /locus_tag="LZB08_002976"
     CDS             3266..3715
                     /locus_tag="LZB08_002976"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBM7316458.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3730..4344
                     /locus_tag="LZB08_002977"
     CDS             3730..4344
                     /locus_tag="LZB08_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7316459.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4433..5989
                     /locus_tag="LZB08_002978"
     CDS             4433..5989
                     /locus_tag="LZB08_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBM7316460.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLKFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5979..7241
                     /locus_tag="LZB08_002979"
     CDS             5979..7241
                     /locus_tag="LZB08_002979"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBM7316461.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7315..9102
                     /gene="aspS"
                     /locus_tag="LZB08_002980"
     CDS             7315..9102
                     /gene="aspS"
                     /locus_tag="LZB08_002980"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBM7316462.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3701 a          975 c         1770 g         2688 t
ORIGIN      
        1 tataataata ttattcttta tgattattta tacaataaag gcgaatattc gtatatatat
       61 gagaataaaa gagaagaaag tgaaattata aaatattata ataaaacaga cctattatat
      121 ttaaagaatg tagttagtaa tatagttcca tcaagagatg aatttataac aatttataaa
      181 caagcactca taaaaaaaga aattgatttg gatatggtaa atataaggga aacttttaat
      241 gtaattccat taaagttttt tacgatatta aatgtattta gagaattaaa tttacttgac
      301 tttaatttaa attatgaaaa aaattctgta ctcataagaa tattgccaaa accacagaaa
      361 aaattagact taaatgagag tttaatactc aataatctaa aaaatttaga aaaacaatac
      421 aatagcagtt attaggaggc gtttcccatg gatttaaaaa actttataag gaatattgat
      481 gatttcccaa agccaggcat agattttaaa gatgtgacaa ctttatttaa agatggagat
      541 gcatttaaat atgcagtgga ttctatagtt gaagaattga aagataaaga tgtagattta
      601 gtaataggac cagaagcaag aggatttttg atgggtacac ctgtagcata tgctttagga
      661 gtaggatttg ttccaattag aaaacctgga aagttaccag gtgaagttga gagttatgag
      721 tatggattag agtatggtac agatacttta gagatacata aggatgcaat taaaaagggt
      781 caaaaagtag caatagtaga tgatttattg gctactggtg gaactatgga agccgctgcc
      841 aagcttgtag aaaaattagg tggagaagta gtttctatgc aatttttgat tgagctaaaa
      901 ttcttaaatg ggagagaaaa gttgtctaac tatgatgtta attctcttat aaaatactag
      961 tacttttata atagttggta taacaccaac tattttcata tatattaaaa ttttatacaa
     1021 aataggtggt tatatgcatg ataaagaatt acaagaaata attgaaaaaa taaaagttta
     1081 tgcacctaat gcaaattttg aaatggttga aaaagcctat aatcttgcaa aaaaagcgca
     1141 tgaaggacaa tatagaaaat ctggggaacc atactttatt catccattag cagttgcaaa
     1201 tattttaata gagatggaat tagatattga aactataaca gcaggtttac ttcatgatgt
     1261 tgtagaagat acagaatata cctatgaaga cataaaaaaa gaatttggca aagaagtagc
     1321 ggatttggtt gatggagtaa ctaaacttgg tcaaataaaa tatcgttcga aagaagaaac
     1381 tcaatctgaa aatttaagaa aaatgttttt ggcaatggcg aaggatataa gggttatact
     1441 tataaaactt gctgatagac tgcacaacat gagaacatta aaatacatgc ctccagaaaa
     1501 agcaaagtat aaagcaaaag agacacttga aatttatggt ggtatagctc atagattagg
     1561 aatttccaag ataaagtggg aactagaaga cagagcatta agatttatgg acccagaagg
     1621 gtattatgat ttagttagta gagtatcaat gaagagaagt caaagagagg attatattca
     1681 aggaattgtt gaacttctta aagataagtt taaagattta agtataccat gtgatgtata
     1741 tggtagacca aagcattttt acagtatata tagaaagatg caaaaaaaac ataagacttt
     1801 tgaagagatt tttgatttaa cagcagtaag gatattggtt gataatgtaa aggattgcta
     1861 tgcagtactt ggtatagttc atacattatg gagacctatt ccaggaagat ttaaagatta
     1921 tatagcaatg ccaaaaccca atatgtatca atcattacac accactgttg taggtcctga
     1981 tggagaacct ttagagatac aaataagaac tcatgagatg cataatatag ctgaaaatgg
     2041 tatagcagcc cattggaaat ataaagaagg tatctctagc aaagagtcaa aggttgaaga
     2101 aaaacttcaa tggttgagac agatgatgga gtgggaaaaa gatttaaaag acccacaaga
     2161 gtttatggat gcacttaaag aagatgtatt taatagtcaa gtttatgtat ttacgccaaa
     2221 aggagacgta attgaattac cagcagaatc aacacctata gattttgcct atagagttca
     2281 tacaaatgta ggtaataaat gtgtaggagc taaaattgat ggaagaatag ttcctattga
     2341 ttataagctt caaaatggta atattgtcga ggtacttaca tcatctaatt ctaatggacc
     2401 aagtagggat tggcttaata tagtgaagac cccaaatgca aaaagtagaa ttagacaatt
     2461 tttcaagaag gaaagaagag aagaaaatgt tgaccgtgga aatacaatat tagaaagaga
     2521 atttaagaag catgatatac cacaaaaaga ccctcttgtt gaaaaatata tggcattagt
     2581 tgctaagaaa tttaatcagc caagtgttga ggacttaatt gccactgtag gttatggtgg
     2641 tataatgtca tctcaagtgg tatctaaggt aaaagaccta tatttaaaag aggttaaaaa
     2701 agctaaaaag gaacaacaga tacaagaaga agatttaaat aaacacaata taagtgaaac
     2761 agaatacagt aaaaagagaa agaaaaacgc atcacaagga attatagtaa aagggcttga
     2821 caatatctta gtaagatttg ctaagtgttg taatcctctt ccaggagatg aaattgtagg
     2881 atatattact aagggtaggg gagtagccat acacagagct gattgcccaa atgcaaacaa
     2941 taatggtgat ttcttccaaa atagattggt ggaggtttca tggagttcag caggaaaagg
     3001 taagtttgaa gcggaaattc aaataaaagc tattgacaga agaggaattg ttaatgaaat
     3061 aactcatatt gttgcaatgg ataaacttag tttaaacgga ataaatgcaa gaaaaggtaa
     3121 ggatagtatt gtaagtataa acttacttgt agaggttaat gacacagagg aattaaaaat
     3181 tctaatgaaa aaattaaaag caattcctgg tgtagaaagt atttatagaa tgacaaatta
     3241 gagttaaaat aatagggagg aaactatgag agcagtagtg caaagagtat cttcatctaa
     3301 agttacagta gatgaaaaca ctataggtca aataaataag gggttactag tattgttagg
     3361 agtgactcat gatgataaat caagtgatgt agattatatg attgataaaa tattgaatct
     3421 gagaattttt gaagatgaaa atgacaagat gaatttatct ctaatggata taggtggaga
     3481 gcttttagta gtatctcaat ttactttata tggagattgc agaaaaggaa gaagacctgg
     3541 atttagtaat gcagctagac cagaacttgc aaataacctt tatgaagaat ttgtaaaaaa
     3601 agcaaaagat aaaggtgtga cagttggtac aggtcaattt gcagctcata tgatggtaga
     3661 gttaactaat gatggtccag taacaatact attagactca agtaaatctt tttaaggggg
     3721 aaaactttaa tgattataaa aaaatttgta gatagttatt ttggagtaaa tacttatgta
     3781 ttaggtgatg aaaagactaa aaaatgtgca gttatagacc caggtggaag tttagtagag
     3841 acattaagtt ttgtaaaaga aaatgaatta aatattgaat atattatcct tactcatggg
     3901 catggagacc atataggata tgttaaggat attaaagaaa aaactggtgc taaggtagtt
     3961 gcacatgttg atgaaaaaga attattaaat gataaaaata aaaatctaag ttatacaatg
     4021 agatgtggag cacaagagtt tgatgcagat atctatgtaa atgataaaga caagcttgaa
     4081 cttggtgaat taaaaatgac tttcttgcat actccaggac atactcaagg atgtatgtgt
     4141 ataagagttg aagatgaaat gttcacagga gatactttgt ttgctggaag tattggtaga
     4201 actgatttat atagtgggga ctttaaccaa atggaaaaat cacttaagaa attatgcaaa
     4261 tatgaagatg ctgttagagt atatcctgga catggaccaa ccagtactct aggggttgaa
     4321 aagaagacaa atccttacat gtaattttat attagtattt aaaaaagtat aaaatattta
     4381 aactatatta gtgagggggt tcttctactg ttaaatagaa ggagttaagt tgatgttgta
     4441 tgttttttta aaaggacatg attataagta tgaagtagca gaacttataa aattgtttac
     4501 cagtgaattt aagtttaaag actctaatgc taacaataat atagagtcaa agcatttaat
     4561 aaatagattg atttatgaga atggtgtatt attctcaagt actgaatact atgaaaatgg
     4621 aaacttaaag tacgagtcaa tacagaatat agaaaatatg aatcttaaat ttttagatgg
     4681 atttgatagc agcaaaacga ttgaagaatt tcttaatagc ctagatagtg aaagtaagag
     4741 gaactttaaa aagctgtgta aagaaaatat caaaaaaagc atgtttgtag tgcttaaaca
     4801 agtatttaat tcttatgtac catggggaat tttgactggt attagaccag tcaaaattgt
     4861 ccacaattta atggataaaa agttagatga taatagcata agaaccattc taaaagataa
     4921 ttattttata atagatgaga aaatagattt agctcttgaa atagctaaaa gagagcgatt
     4981 atttatgtat cctatagata aaaataaaat ttctctttat gtaagtattc cattttgtcc
     5041 aactagatgt gtatattgtt catttccttc aaattcatta aaacaatttg gacatttaaa
     5101 aagagaatat gtttataaat tgattgaaga aattaagggg tttgccaaag ttattaagga
     5161 gactaagaaa gaagttgaaa ctctatatat tggtggtgga actccaacta cacttgatga
     5221 ggaagaattg gatttgctta taaattcatt gttcaatgaa ttagatttaa gtaaaattaa
     5281 agaatttact gtagaagcag gtagacctga cactattaca gaacaaaaac taagagtgtt
     5341 aaaaaaacac aatgttagta gaattagtat aaatcctcaa actatgaatg atgatacctt
     5401 ggtaaagatt ggaagagagc atagagtatc tgatttagta gattgcttta atatggctcg
     5461 tagaatggga tttgataata taaatatgga tataatttta ggtcttatag atgaagattt
     5521 aaatatggta aagaatacat tggaggagat aaaaaaactt tctcctgaaa gccttacagt
     5581 tcatacatta gccataaaga gagcatccaa cttaaatata aatatggata ggtataaaga
     5641 acatctaaca caatatgaag aaatggtaaa aatgatagac ttatcaatga aatatgcaaa
     5701 agatatggga ttaaatccat attatatgta cagacaaaaa catatgttgg gaaatttaga
     5761 aaatataggt tatgctaaag aaggatatga gtgcatttat aatatacaga ttatggaaga
     5821 aaaacaaagt aactatgcat taggtgcagg tgctatatcg aaatttgttt atgtagatga
     5881 agatagaatt gaaagagtag aaaatgtaaa aaatgttgaa caatacattg aaagagtaga
     5941 tgaaatgata aaaaggaaga aagaagaggt atataaaaat gttgactaaa gctcctagag
     6001 gaactaaaga cataactcca aaagaggctt acaaatggcg ttatgtagaa aataaattta
     6061 gagaaatatg tgctttatat ggatatgaag aaatggtaac acctatattt gaacatacag
     6121 aacttttcaa gagaagtgtt ggagatacta cagatatagt tcaaaaagaa atgtattctt
     6181 ttaaagataa aggtgataga gaaattaccc taaagccaga aggtacagca ggtgtagtaa
     6241 gagcatttat agaaaataaa ttatatgcag atactcaacc aacaaaatta ttttatgtga
     6301 caccttgttt tagatatgaa agaccacaag ctggtagaca aagacaattt caccaatttg
     6361 gaatagaagc acttggaagt gatactcctt caatggatgc tgaaataatc gcactagctg
     6421 ttcaattttt taatgaagtt ggattaaatg atttggtagt gagtataaat tcagttggct
     6481 gtccagtttg tagaaaagag tacaatgctt tattaaaaga gtatcttgac tcaaaagctg
     6541 atatattgtg tgatacttgc aatgagagaa gagaaaaaaa tccaatgagg gttatagact
     6601 gtaaaaatcc tacatgtaaa gaaaatatca aagatatacc atttattgca gaccatttgt
     6661 gtgatgattg taaatctcac tttgataaac ttcaagaata cttaaaggaa atgaatataa
     6721 actttgtaat tgataaaaca atagttagag gcttagatta ctatagaaaa actgcttttg
     6781 aaattatatc taatgatata ggagctcaaa gtactgtatg tggtggagga agatatgatg
     6841 gtcttgttga acaattagga ggaccaaaag gtataagtgg aattgggttt ggactaggaa
     6901 ttgaaagact tttacttaca cttgaaggta atggaattga aatagaaaac cctcaatcta
     6961 cggatatatt tatagtaact ataggtgaag aagcaaatac aagaagcttt aaattgttaa
     7021 aagacttgag acaaaatcat ataagtgcag ataaagacca tatagagaga agtgttaaag
     7081 cacaatttaa atattcagat aaaataaatt caaaatttac aatagtaatt ggagatgatg
     7141 aacttaaaaa tgacactgcg acattaaaaa atatgaaaac atcagagcaa actactgtaa
     7201 aattaagtac attagttgaa gaattaaagc aaaagctgta aaataagaaa gtatgatgtt
     7261 ttgtgtatta gataaatata tagcattaaa attaaataaa atggaggtag tatcttggaa
     7321 actttaaagg gtttaaagag aactcactac tgtggagagt tgagagaaaa aaacataaat
     7381 gaagaagttg tacttatggg atgggtacag aaaaaaagaa atttaggtgg tcttgtattt
     7441 gttgatttaa gagatacaag tggattatgt caaatagttt ttgatacaga tgtagataaa
     7501 gaagcttttg aaaaagctga aaaattaggg gctgaatttg taatagctgt aaaaggaaaa
     7561 gtatgcgaaa gacaatctaa aaaccctaat atgccaactg gtgatataga aatatttgca
     7621 actgaactta gacttttaaa caagtcagaa actccgccta tttatataaa agatgatgat
     7681 gatgtttctg aagcactaag attaaaatat agatacttag atttaagaaa accatctatg
     7741 caaagaaatt taaaattaag acataaagta atgaatatta caagaaatta cttatcaaac
     7801 aatagatttt gcgaaataga aacaccattt ttaatagctc caacaccaga aggagcaaga
     7861 gattatctag taccaagtag agttaatcca ggaaaatttt acgcattacc acaatcacca
     7921 caattatata aacaattatt gatggtaagt ggtatggata gatatttcca aatagttaaa
     7981 tgctttagag atgaagactt aagagcagat agacaaccag agtttacaca aatagactgc
     8041 gaaatgtctt ttgttgagca agaagatgtt atgagtatga ttgaaggttt gcttgaagcg
     8101 atatttaaag aagttttaga tgtagaattg gcacttccac ttcctaagat gacttatgca
     8161 gaagctatgt ctaaatatgg ttcagataaa ccagatacta gatttggata tgagttaaca
     8221 gatatatctg atgttgtttg taactgtgga tttaaagtgt ttgctgatgc tacacaacca
     8281 ggaaaaagtg tgagaggtat aaacgtaaaa ggcaaagctg acgactttac tagaaaacaa
     8341 atatcatcac tagaagaaca tgctaagaca tatagagcaa aaggtcttgc ttggatgaaa
     8401 gtaggacaag aaggagtaac ttctccaata gcaaaattct tcaatgaaga agaaatgaac
     8461 gcgatactta caagaatgaa tgctgaagta ggagatttac ttttatttgt agctgataaa
     8521 aactcaatag tatttgatgc tttaggacaa gttagacttg aagtagcaaa taggcttaat
     8581 ctattggata aaaatgtata taacttacta tgggtaactg agttcccagt ttttgaagaa
     8641 gatgaagaaa caggcacatt ctcagcaatg catcacccat ttacatcacc tatggatgaa
     8701 gatttagaca agttagaaga aggtgataag tcatctttaa gagcgaaagc ttatgatatt
     8761 gtattgaatg gttatgaaat aggtggagga agtgttagaa tatcaaattc tgatgttcaa
     8821 tcaagaatgt ttaaagcact tggttttaca gaggaaagag ctaatgaaaa gtttggttat
     8881 ttattagaag catttaaata tggtactcct cctcatgctg gtctagcttt tggtcttgat
     8941 agacttgtta tgttacttgc aggaactgat aatataagag aagttattgc attccctaaa
     9001 aatcaaaatg ctgtttgccc tatgacaaat gctcctacat tggcggaaga tgagcaatta
     9061 gaagaattaa gtataaaagt tgatataaaa gataatgaat aatatgtagt tgcaagaaac
     9121 taaaatgtaa aaag
//
LOCUS       DAFKMX010000161         9057 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFKMX010000161 DAFKMX010000000
VERSION     DAFKMX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.162"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            232..1851
                     /locus_tag="LZB08_002981"
     CDS             232..1851
                     /locus_tag="LZB08_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBM7316463.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2225..2419
                     /locus_tag="LZB08_002982"
     CDS             2225..2419
                     /locus_tag="LZB08_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7316464.1"
                     /translation="MINYMVEIIYSEKISEIENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2978..3301)
                     /locus_tag="LZB08_002983"
     CDS             complement(2978..3301)
                     /locus_tag="LZB08_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316465.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3346..3600)
                     /locus_tag="LZB08_002984"
     CDS             complement(3346..3600)
                     /locus_tag="LZB08_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316466.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3864..4145)
                     /locus_tag="LZB08_002985"
     CDS             complement(3864..4145)
                     /locus_tag="LZB08_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316467.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDIVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            4458..5267
                     /locus_tag="LZB08_002986"
     CDS             4458..5267
                     /locus_tag="LZB08_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316468.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5309..5575
                     /locus_tag="LZB08_002987"
     CDS             5309..5575
                     /locus_tag="LZB08_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBM7316469.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            5857..6621
                     /locus_tag="LZB08_002988"
     CDS             5857..6621
                     /locus_tag="LZB08_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBM7316470.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            6684..6968
                     /locus_tag="LZB08_002989"
     CDS             6684..6968
                     /locus_tag="LZB08_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBM7316471.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            7116..7658
                     /locus_tag="LZB08_002990"
     CDS             7116..7658
                     /locus_tag="LZB08_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBM7316472.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(7821..8990)
                     /locus_tag="LZB08_002991"
     CDS             complement(7821..8990)
                     /locus_tag="LZB08_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBM7316473.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
BASE COUNT         3389 a         1211 c         1149 g         3308 t
ORIGIN      
        1 acataaaata ctaaaaatct aagttcaagt atttaaattg ctatatatca taaattttct
       61 aaaattaaca acaacttaaa aatttaacat tctcttaacg tatacttaac aaaacattaa
      121 tatttttatt gacaaaattt gttattctat attatggaaa caataaattt tagttaataa
      181 agtttataat tcagataaaa aaattattaa tttcttaagg gaggaaatat cgtgaatata
      241 gtaattagcc ttatcggtgg acttggactt tttctttacg gaatgagttt aatgggagaa
      301 ggtttacaaa aatcagcagg agacaaatta aaaaaaatta ttgaactctt aacaagtaat
      361 gttgttatgg gtgtactggt aggtacagtc gttacaggta ttatacaaag ttcaagtgct
      421 actactgtta tggttgtagg atttgtaaat gcaggtataa tgaatctaag tcaagctatt
      481 ggtgtaataa tgggggcaaa tataggtact acagttactg ctcaattagt ttcatttaat
      541 cttgaaggaa ttgcaccaat tgctttaggt ataggtattt tattctattt atttacatca
      601 aatcaaaaaa caaagcattt atctgagata cttataggtt tcggtatatt atttacagga
      661 atggagttta tgaaagatgc agtagctcca cttgctgaat ataaagcatt cactgatgca
      721 cttttatatt ttagtaaaaa tcctgtatta ggtatattgg caggatttgc cataactggt
      781 attgttcaaa gttcaagtgc atctatgggt atgttaatag cactagcttc tcaaggaata
      841 cttcctctat catctgcttt acctattctt tatggagata atataggtac ttgtgttact
      901 tcacttttat caagtgttgg tgcaagtaga aatgctcgac gtgcagctgt aatgcattta
      961 agttttaatg taattggtac aataatattt atgcttgttt taaataaacc tatatcagcc
     1021 atagttactc attttgaccc aactgataca gctagacaaa tagcaaatgc acatacttta
     1081 tttaatctta caaatgttat aatattatta ccattttcta aatacatagt taaattagca
     1141 aatagactta tacctattaa agaaactgaa tcagaaatag taaataatac taagtactta
     1201 gatgaaagga tgtttagcac tccatctata gcacttggta atactgtaca agaagttgta
     1261 agaatgggtc ataaagctac aaactctttg gaacattcaa ttgcaggatt tttaaataag
     1321 agcaatgaag atattaataa agcttttgag tcagaaaaag ttgtcaataa gttacagaaa
     1381 gatatattga actatttgtt aaaactttct aaagaacctc ttagagatga tgaacgcttt
     1441 agaacagatt tgctttttaa tactgtaaat gacattgaaa gagtctctga ccatgcagaa
     1501 aatatagctg aattggccat gagtgttaag gaaatgaaca ttagtttttc tgatagtgcg
     1561 ataagagaaa tatacgaaat atacaataag acaattacta attttaagga tgccttaata
     1621 gttttagatg ttaaagattt tgagttagca aataaagttt tagaagttga aaatgaagta
     1681 aattatttag aaaaaacttt tagaaattca catatgatta gactaaataa tggaagttgt
     1741 acaattgatg ctggtgtact ttatcttgac ttacttacta atctagaaag aatttctgac
     1801 cactcaacaa atatagtaaa acaagtacta aaacttaaac aaaaaatata gtgattattt
     1861 ttatatataa tcttaatcaa tgagaagtaa gaagctatag ataatgctta tatcatttat
     1921 ggcttcttat tttttatgta atactcaaat ttttatagta ttctcttatt attaaacagt
     1981 ttctgcctag aatattccat atatatatga cttctttcat aaaacttgaa ctgcccataa
     2041 ataaagtcac aagtatacat aactattttt taatcaatta taaccaatat ttgttttatt
     2101 tatcttcatt ttaattcttc attcaataat gtaataagat tattttttat ttggatacta
     2161 ttatatcatc taaatatgtt attatatatt tggatatttt catattaata ggagggattc
     2221 tacaatgata aattatatgg tagaaattat atatagcgaa aaaataagcg aaatagaaaa
     2281 catgttgaaa attgaaaata tacaatatga atttacttct cgtgatggct ttatattttt
     2341 tggtttttat gatatgacag acgcatatag atttgagact ttatgttcag atgaaaaaat
     2401 tcgatttaaa agaatataat agataaaaaa gtacatattt ctaaactatt tgtttttcaa
     2461 atttaggttg tgttttaatg tagtgttgat attataaaca attttaataa taaaaaagta
     2521 gggacattat attaatatgc tcctactttt tactcatact tattaatatt atttatcaaa
     2581 attaccctac aaactggaat ttatcattct ttcataattt taaactatca tatttaaaat
     2641 atataaaatt ctacatgatt tttgtataaa cataatatca accattatcc aatttaaaaa
     2701 tattttttag taatttccat ttcattttct cttaacatat taattaaact tttatatgta
     2761 aatggtgtat ttacatatta tttaatatcc aacaattata taaagaaatg tgctctttgc
     2821 cttataaaga attataaagc caattcgttt tattatatta tttttcttta taccttaaaa
     2881 actttacctt caaattctta tttatctata tattataaaa atgttcttaa cctcttttac
     2941 gagattttct tgacatatta tcagttagat aaaataatta aataatataa tcagcaagta
     3001 tgttttttac tcttttaagt aaatttccac ctgcacccca atcaattttt aaaattccaa
     3061 ttgcactgcc tgttgaagta gctttaataa ctgtttttcc tgtggtattt tcataaacta
     3121 ctattaatcc tgcatcacta ttatttcttg cataatactt ctcaaaagtt gtaattagta
     3181 taaatttatc atctcctaaa ttataagaat cactaaaaac taaatctgca tcaagatttt
     3241 cttttatcat tttttgtgcc tctaagggtg taatttttac agtaaatatt tctgttgcca
     3301 ttttttatct cctcataaga atttatttta gacaaaacat aatctctagt attttctgat
     3361 atatttatca aacaatatcc ctattcctcc taacagagct aatatagaac caaaatttct
     3421 atattgagaa atagattggt ctagataaat attatattgg tcagtatcca tgattccacc
     3481 attttgttga ataatatgct ctgcactaaa ttcacctaaa cctatacaac agaataatac
     3541 tactactcca attacaaaag caaatataca tattgttaat ttaaaatcct tattttccat
     3601 agttatccct ctcataagct aaatttattt gattattttt actagatttt atattactct
     3661 tattatacaa tacaacatat atttacatct atttccaaaa ttgtaacctt tatagtttta
     3721 tatatttaaa ttttttaaac aaaaaataca catctataaa ttataaatgt gtattctgta
     3781 ccattactaa taatatatta tactttttta caaatttcta attgtttata actattcaaa
     3841 aataaaattc atataaaata attctattgt acaattgaaa ctttaataat gtctccattt
     3901 tttctctcca agtgaactac ttctccagtt aaatcatagt caaatgcttt aactaatatt
     3961 tcagtaactt ttttaccatc tactatatca tcttttccat taaataaatc caaagcatta
     4021 ttctcaatca ttctctttgc aaaatttaca ggtaacttta tatgtatttt atcgcctgta
     4081 tgtgataaga ctcttattct taaaaacttt tctaaattgt ttcttggtgt ttgttttgat
     4141 gccatgctat taaatcactc tcctttattt tttatttaat aataaatttt attcttaaaa
     4201 atatctttat atgtatttat tatgaaagta acactattat tttctaatct tgttttatat
     4261 tccttcttct ttaatatatt tatttaataa atatctatga tttataataa ttgtatattt
     4321 tgagatttta ctttacaatt attgtatcta aatttaacaa aatatcttat tagaatattt
     4381 caattaaaac aaaactggtt tattatataa aaaataaatt agtatactta ttttttatat
     4441 atcaggagga tatacttatg aataaaataa tcaataacat tttaaatacc tgcaaacagt
     4501 atacatatat tttgccacat aaattgttaa gacagtatat agcacactat acaatatcta
     4561 ttccagatac aagtattaaa gaaaatctta cattaatacc tgatgctagt ggatgtatga
     4621 tatttaaatt tgacaaaaaa ggtatagaaa gcgcattctg gggagccaca acaaaaacta
     4681 ctattgtaaa aaatgatata gaaaatgtac tttttagagt attcgttgag tttcgcccag
     4741 gtggagtata ttatttgaca ggtctttctc aaagagaatc tacagattta aaaatttcac
     4801 ttgaagattt taacacatta tttagcttag aggttaattc catttttgaa agaacaagta
     4861 ctataaagga attagtagaa caattagata tgttattctt atcatactta ttgaaatcaa
     4921 atatagtaga tatgactata ccaattcttg aaaatgctag aaaacaaaac tctattatgt
     4981 ctgttaaaaa catttcacaa attagttgtt atagtgaacg acatttaaat agaattttta
     5041 ataattctct tggtatgagt gttaaatcat accttagatt acttcgtata aaccttgtat
     5101 tacaagaaat acaaaataat aaaataccat ttgcaacttt agcacaagat attggttatt
     5161 acgaccaatc tcattttata aatgacttta aatctatttg tggtgttaat ccaacaacct
     5221 atataaaaaa tttgtccgat ttttacaatg aaaagtataa attttgatat acaatcatat
     5281 aaaatttata tttaaggagt gacttaaaat gatagaatca agatgtggaa ttttatgtta
     5341 tgaatgtgga tacaaggaaa aagtcaattg taaaggttgt acagagattg agaaaccatt
     5401 ttggggtgaa caatgtcctg taaaatcttg ttgtgaagat aaaaacctta cacattgtgg
     5461 tctatgtgat acatttccat gtgaaatgct taatcaattt gcatatgaca aagaacaagg
     5521 tgatgatggc aaaagaattt ctcaatgcaa aaaatgggct gtactagcct tataatatac
     5581 tctcttgtta taaaataagt ttttgtaagg taaatttttt aaactgttgt agaaatttct
     5641 gcaacagttt ttataattaa atttaaaggt tatttaatta aattaataga atagtaattt
     5701 attgataaaa tctgattact aaaattggaa atataaaatc tcaatctctg tctatttaaa
     5761 atatatgtta caaaattgaa ttaaaataga cttaaatttt attttatgtt aaaatcttta
     5821 cagtaataaa taattttatt ttaaggagat aattttatga ataattactt aaaatttgct
     5881 acatatcaac ttataggttt tacatcttct atacctaggc ttattaaaat taaaaagaat
     5941 cctgataaat ttagcttaaa ggaaaaattt gaatttatgc aaaaacaagc taaaaaatct
     6001 ttagatatag tgaacataga gcttaatata attggtaagg aaacactgcc aaaagaacca
     6061 cttctatttg ttgtaaatca ttctagtatg cttgacagct tcatactaac agcaagtgta
     6121 gaaagaccaa ttggatgtgt aattgcagat gaaccagttt ggagaaatat acctatattt
     6181 aaagaatggg caaaattact aagatgtgta tatgtaaaca gaaaaaacaa tcgtgagggt
     6241 ataaagagta tagcccaagc ctctcaaaat atacttacag gtcaaagtat ggcagtgttt
     6301 ccagaaggag atttaacatg gattaaagaa ccaaattcat tggtttcgga gtttagaagt
     6361 ggagctttaa agatagcata taaagcaaaa tgccctattg ttccactagt aataaaaaat
     6421 tcaaaagata cttatgaagg ataccaacct attggtaaaa ttaactctgt tcctgtcgaa
     6481 gtcgaatttt tagaaccaat ttatgaccat atcgaaaatc ctagactaaa gtcttctgta
     6541 ctaggtgaaa atataaaaaa taagatgata aatacaatag aaaattttag aaaatcaaat
     6601 aaaacattta aagaatttta atattaaaat ttattgacta aaaaaaattt taatgttaga
     6661 atttttttta agaggtgatt aaggtggcaa aaaatacaaa gcaaacaacg gttcatattg
     6721 atgagactct acttaaggaa gttaagagaa taggaattaa tgaaaataaa agtatatctc
     6781 aaataataaa cgaatcaata atagattata tattagtata cagagaaaac gaagataaag
     6841 aaaaaaatga acatgataag aatctaaatg aaacaataaa tgaatttaaa aaagaagaag
     6901 aaaaaagaat gcaagcaaaa agtgctgcta gagctatagc agaagcaaaa aaacgagttc
     6961 tttactaaga aaaacaaaaa tcggtacaaa ttaagcaccg atttttttat attaaaaaaa
     7021 ttacatatga agaagttttg aaagaattat aaaagatgca aattccagtt tgtactacat
     7081 agaaaaatgt gaatgtttag aggtgaaatt attatatgaa aagttatatc gcattattaa
     7141 gaggtatcaa tataagtggt aagaacaaga taattatgtc tgaattaaaa gcaagctttg
     7201 tagaacttgg ttattcagaa gtttctacat accttaatag tggtaatgtt gtctttttaa
     7261 gtgatgtaga tgacagcaag gttatctcaa atacaataag attaatgata aaaaaacagt
     7321 ttgaattgga aataccagtt tttattattt cacaagaaga gttaaaagat atactaaata
     7381 atgctcctaa ttggtgggga gataacaaca aagaaatata tgataatctt atttttatgt
     7441 ttcctgactt atcttatgac gagttttatg atgaggttgg aaatcctaaa gaagagtatg
     7501 aaaaagtata tcattataaa aatgctatct tttggtcttt tagtcgtaaa gattataaaa
     7561 agacaaattg gtggtcaaag actgccagtt caaatgtaag tgataagatt acaattcgta
     7621 cagcaaatac ggtaagaaaa atagttggaa tgcgataaaa gataacaaac agccgataaa
     7681 cctcaaaaaa taggtatata tcggctgttt ggaaacttat aagaatatag tcaaaagtta
     7741 tgctgttttt attgataaaa taagtttaaa aatgagtcaa gaaatattta tataattctg
     7801 gaaatactta tatttattct ttaaattaaa tcaccaacaa cttttacttt tataagaagt
     7861 gcattaccag gaaggtatgc aagtggaaca tcttctatat ttaatacttc tatagtatct
     7921 tctttagcac cagcttttag agctttattt acagcctctt tttttgcatc ttctatggca
     7981 tcttctctcg acattttatc taaactgtat actttttcta catctccact tatttgtcct
     8041 aaagctgcac caatagcatt tgcagcagta taatgaggag gtcttataac attgcttatg
     8101 ccttttagtt tttcaggaac aattatactt cctccaccag ttaaaaccaa ttcaacatca
     8161 tcttgactag tcttcatttg gtctacagca tcttctaatt ttaaaataac cttcttatat
     8221 acctcattac aatactcttt atctaagttt tcaacattgc tttttctatc ttcaaacgcc
     8281 atatctaatt ttgtaacaac atctgataaa gttagagtgt ctccaccaaa tattattgca
     8341 tcttcagtta ttctatatcc cacactttca ggacctatct ttatattatc tttttcgcct
     8401 tttacacatg taccaccagc aaggccaata gttaaaacat ctggcattct aaagtttgta
     8461 tgtactcctc caattgtaac tggaatagat gattctctag ggaatccatt atatagtaca
     8521 cccaagtcgc ttgtagttcc accaacatct aatactattg cattttttac ttttgataag
     8581 aaggaagctc ctcttataga atttgttatt ccacatccaa ttgtaaaaat tgggtatttc
     8641 attgtatact ctaaattcat taatgttcca tcattttgac tgaaatatat ttgagcatct
     8701 atacctaagt ctttaagtgc tttttcaaat cctaaagata cagctttacc tacttgagac
     8761 aatgcagaat ttaaaatagc tccattttct ctttctaata tacctatatt acctatttca
     8821 tttgataaag atataggaat atcccctaaa atttctctaa cccattttgc aacttcttct
     8881 tcttgttgtt tatttactgg agcaaatact ccacttattg caacactttc cactttacct
     8941 tttattctat tgcaaaactc tacaacttca tctttacaaa gttcatttat ctttctaccc
     9001 tgaacctccc atgactaaag tcacagggtt cctgcttcac agaattttgc atactaa
//
LOCUS       DAFKMX010000162         9023 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFKMX010000162 DAFKMX010000000
VERSION     DAFKMX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.163"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(50..2116)
                     /locus_tag="LZB08_002992"
     CDS             complement(50..2116)
                     /locus_tag="LZB08_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBM7316474.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQNEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2193..5222)
                     /locus_tag="LZB08_002993"
     CDS             complement(2193..5222)
                     /locus_tag="LZB08_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7316475.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIMSI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWVRLNIFEDELKILGVVVDVTK
                     EFIEKKKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMKTLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5275..6735)
                     /locus_tag="LZB08_002994"
     CDS             complement(5275..6735)
                     /locus_tag="LZB08_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBM7316476.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6827..7201)
                     /locus_tag="LZB08_002995"
     CDS             complement(6827..7201)
                     /locus_tag="LZB08_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316477.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7377..8117)
                     /locus_tag="LZB08_002996"
     CDS             complement(7377..8117)
                     /locus_tag="LZB08_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBM7316478.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8138..8926)
                     /locus_tag="LZB08_002997"
     CDS             complement(8138..8926)
                     /locus_tag="LZB08_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBM7316479.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEVATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKTINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
BASE COUNT         2880 a         1613 c          867 g         3663 t
ORIGIN      
        1 gcacataata aataatacat tatatttcta tctaaattta gattctaaat taaatagtat
       61 aaattatatt cttcaataaa caagttttca aatccaggat tcgctctcta tctttaaact
      121 ttattgtaaa cataattcca tcttttttaa ttatttctcc ttcatgaaat tttttatggt
      181 ataccttatc tcctacacca atactgtcaa tttctttttt cgttggagat tttatatcat
      241 taataaataa tgatttatca actttcttac catacttatt taaagtagag cttatacata
      301 acttttcttc agctcttgta atagcaacat acataagtct tctttcttct tctaattgtt
      361 cttctttttt ctcttcctta caaatatcat atgatttttc atgaggtatt gttccttcat
      421 ttacacctat aatataaaca tttctaaatt ctaatccttt tgcactatgc atagttgtaa
      481 atatgacacc ctcattctgc ttattcttgt tattttccgt taactcagtc tttactctat
      541 caatatgctc taaaaactct gtaacagtat taaaattagt tgctgaactc tcaagttcat
      601 ttaatatctc tacaagtcca ttagtcttta tctttctgtt gctacaataa tctaaaacat
      661 atctatcata atcaagacta gttctaatat aagatattgc atttttagga tttaaagtat
      721 ttaaataact taaatctatt tccaaatcat ttattgtttt aacttgttta ggatgaagat
      781 tacacttatt tattaatgct gttataaagt ctttttcatc cttaaccata tttatactgt
      841 ctttggatat atatctaaaa ggcttgttta taattctcaa ccaatcttta tttgattttg
      901 gattaatacc tattcttaaa taggctagta tatcttgact tgcccaatgg tcatatattg
      961 taattactga gtcttttact acaaatggaa tcctcatatc cataaataca tctactaatg
     1021 ctcttgactg tatattagtt ctataaatta ctgcaaaatc tgaatactct acataatctt
     1081 tttgtatttc gtcaattatt tccctggcaa tatataaggc ttcttcctct gaatcatgcg
     1141 gagaaatata ggtaacactt cctccatccc cctgactaca cttaatgatt ttttcatatc
     1201 tatttttatt tttgtcgata agtctatttg cagtatgaac tatctcactt ttagacctgt
     1261 aatttatatc taatattatt tttttagtat tattaaagta ttgttcaaat tcaagcaaaa
     1321 aatctggtct agcacctcta aatccatata tactttggtc ttcatctcca actgcaaata
     1381 tattgtttaa tgggctacaa attaacttta aaacttcaaa ctgtacttta tttatatctt
     1441 gaaattcatc tatcaatata tatttataca catttctaac catttctagt gcagacttat
     1501 tgttcaaaag taaataataa gttcttataa gcatatcatc aaaatctatt ttattaactt
     1561 tagatttttg ttcttcgtat aagttgtata cttttaaaaa ctcatctttt gttaatactt
     1621 ctgaattaaa ttcattttta tccataagtt cattttttac ataggaaatt tcatttataa
     1681 cttgtcctat attctcatca tcatcagcat tctctatatt taaacttttt aggattgcct
     1741 tgattgtcat tcgctttgac ttttcatcaa atatactgtc taagttgtat ctttcaaagt
     1801 atcttagaat tttaaaaaat acagagtgaa aagtaccata ggttacctta ttaagtctta
     1861 tatcatcact taaattcaaa gctctatttt tcatttctat tgaagatgcc ttggtaaaac
     1921 ttattgccaa gattcttgtt ggtgctatgt ttttgtttac aaccatattg gctattctat
     1981 atgtaattac tctggtctta cctgaaccag gccctgcaag tatcatgcaa ggcccatcta
     2041 tatgctcaac tgcttctaat tgattttcat ttaactttct tatatctata tttgttgtga
     2101 ttattagctc caccaccttg atttataatt atattatttt ctttttaatt ataaactaaa
     2161 agttcataaa aataaattta atttttcctc tattaaacca catcattcct attttcatga
     2221 catatcttga gttctttttt aattttgtcg cttatatctt caggtataaa tgatggttta
     2281 ctaatatagt agccttgcat ataatctact ccaagattaa ttagtgtttt catttcacct
     2341 tttgtttcta caccttctgc tatcacttta atatttcttt cgttagaata tgagataaga
     2401 ttttttaata ttttctgtct gttttcatct ttatcaatac ctcttacaat agacatatca
     2461 atctttacat aatctggcat tatatataat agtacagcat ctccgttgta acctgtccca
     2521 aaatcatcta aagcaatttc tatattccaa tggtctatat atgctttctt tttctcaata
     2581 aaatcttcat taatcttatc attctctgtt atttctagta ctactctatt taaatattca
     2641 ccatataaat cttcaatttc ctcaaaatct acttttgaca aattattatt aggaatagaa
     2701 tttaagaaca gtttgcaatc gccaaatttt tctttgtatt ttttaaatga tttaagtgct
     2761 ttaaaccatg taatacgctc aatttgatat aatttagact gtgaacgagc taatctaatg
     2821 acatctattg gcgaactaaa agtttttatt ttaggtctca taagtgcttc atatgcaaat
     2881 actttacctt tacaatctac aataggctga aatgcatact caactaattg accatcaatc
     2941 aacttgttta attcttctct attactcaat aagaatgaat ccttattata tatattaata
     3001 tcaaattcac taacttcacc cttgattgta tttttaattt gatacatagc aaaatctgcg
     3061 tatctcatta attcatcata gttggttgaa tcatcagggt accaggaaat tcctgctgat
     3121 gcccttattt taaattctgt tccatcatga agttttaata aggtattatt catctgtttc
     3181 ttaacagaat taataatatc tcttatacta tctttacttt catatccata tataaataca
     3241 taaaactcat cacctgaaag tctagatact ataccattcc atgatataaa cttcttaagt
     3301 atgtctgcag cacacctaat atattcatca ccagcatcat gtccatatgt atcattaata
     3361 tattttagat tatctaaatc ccacataata aatgctgcta tcttaagttc atctttattc
     3421 ttaaattttt cttgtatctg tgaatgaaaa gctctcctat ttaaaagatt tgttaaaata
     3481 tcaaaatctc tttcatattc tatcttcttt ttctcaataa attcttttgt gacatcaaca
     3541 acaacgccaa gaatctttaa ttcatcttca aagatattca atcttaccca cttaagtttt
     3601 ttatctttag attcaaactt atatatatat gtattttctg tttcataact ttcttgcaaa
     3661 tatttatcta gtccccttaa ttttttaaga aatttatttt tttccatata tccatattct
     3721 tcaaatttga tttcagtagt aaacacacta aaaaattctt ttgtacaaaa aacattttct
     3781 gcgtttttgt ataactcaaa agccccaatt ggcatatcta ccatttcaat aatctgtgat
     3841 aattttgacg aagaatctgc aacatttgca ctgagcaact ctatagctga agataattca
     3901 tctatttcag atatatttat cttatcaaga tttactggtt ttgatggatt actgtttcta
     3961 actttgttgg ctaaaagaac aattggtttt gtaaataatc ttgatgcaat gtaaatacct
     4021 attagaccta ttaataaaga aattgctata gacattataa ctaatttttc tacccttttt
     4081 gaaaatgaca aaagactttt ttcttcaata atacccaaaa gagcccaacg ttcattttca
     4141 aatggagtat tggagttata taaatctaag tattgtacac tagcataaat gttgccatta
     4201 ccacattcat tatctttatt tttaatctta tatatatttt tatacgattc cttctgctct
     4261 aaattagaat gtaaaccatc ccaaaatttt tcttttaatg agtatccatt tgaaattaca
     4321 ttttcaaagg ccatatcatt atttctatct acacctaaaa tataattacc ctttttattc
     4381 ccatttatct ctgagtatgg aagtaattct cttaaataat ttacacttaa atctattcca
     4441 agtactccat atacagttcc atcttcataa ataaggggaa ctgtatatgt cataagccta
     4501 tcacctgcat ttgtcaataa aaacttactt ccccagtatc ctaaattaga acttgataca
     4561 tttggattat caattgcagc tctaaatggt ttataaaaaa aatcatctcc ctcttgctca
     4621 ttttcacttt taaatgaaaa cttaggttcc caataactgt ccatagatat tccaaaactt
     4681 cttgctatat ctgatgaacc tctctcgatt aacaaatcag aattatcatt tggattaaac
     4741 ttagggtcta aatcccttat atacaatcct gtcctactta tttcaccttt actatctatt
     4801 gacttatcgt taatattttt gtcacttagg attataaaag ctcctgtaac agaatttttt
     4861 ctcaaaagat atattaaatc atttgatatg ctttttataa tatctttgtt aatttcttca
     4921 ttgttgccta catcctcaat taaggcattt ttttcattta atactttttt tacagaggaa
     4981 ttaattgtag cttccgtttc acttgtattt gaccatcttt gtaacatttc attttgtata
     5041 taatttttac gattaattac tctttcattt aagatgtcaa acgaattatc atttaatttc
     5101 ttaatagttc ctccccataa aattgtggca caaaataaac ttgtttgaat tagcataaca
     5161 ataattaggg gaaatatcat ctttttcatt attgaattgt tttttcttag attgttttcc
     5221 atactcattc cacctatacc tttgtgcttg tcaacacact aactgccaat tcatctattt
     5281 ttttatagca ttttcaagct cagccttaaa actttcaaac caagttttaa aatttttatc
     5341 attattaaat tgtgctactg catccttctt agaagttcca ctttttacta attcatttat
     5401 tttatttgaa tcttctttag ctttatcaag cattgaatat tcaagtattt gtctagcttt
     5461 agttccacca tcaaatgctt tattagtata taaattacaa ctgttaatct gttcaataga
     5521 tgaggctatt gaaccttcaa ctttatcaga taaactcaaa ttatctttct tcataacttc
     5581 ttttataaca tccaaatcat tcgttttttt ctttactggt aagtatccag aagcaataga
     5641 gaattttata ttaggttctt tatctgtaaa ccattttaaa aattctactg atgcatattc
     5701 ttcttcttta gtagattttg ttacagccat tcctgcacct tgttgtacag catattttcc
     5761 ttctccttta aaacgtggtg cttgaaaaac agatgtttca atattataac tctcattatc
     5821 atttagcatt actctatctg gaaagtatgc tgctcctgaa ctagaaccta caagagctaa
     5881 tatatcacct gtttttgcat catcagaacg aaacttacca taagaagcaa aatatccatt
     5941 tatatatggt acatagaaat tatcccatag ttttttcata actttttcat caatatttag
     6001 tgtaacttta ccatctttaa cagaaaaaat ttctactcct agttgtttac ttcctataat
     6061 aatataattt gctaaagcat ctcttccaaa aaatgcttta ccatcattag gctctttcgt
     6121 taaactatct gtccattcat aatatttttt tgatgtctct actaatcctt caattgtttt
     6181 tatatcattt atatttgaat ttgtagcttt tgcaaattta tcccaatcag ttttatttaa
     6241 catcaaaatt tcagttgatt ttgcaattgg gaaaagttta aactcatcct tagaatttat
     6301 tctgccttct tctacatatt cgtctatgta ttcatttaat tcttctttac ttatgtactt
     6361 atccatatct actagtaacc ctaatttatc catttcatat gcagtatctg agtatgctgc
     6421 aaatatattt ggtacatctt cagaaccaac ttttttattt gcagaatcta aaactttctc
     6481 tgataaatca actacattac cttgactgaa tgcttcaaca ataattcctt tttcagcacc
     6541 tacagtgtca ttaaattcct taacaagctt atcaaaagcc attttctgct gtccattata
     6601 ataatgccaa atttgtattg atgtcggatt ttctgggtct aacttataat tatcttcctt
     6661 ctttaaacta catcctgtaa gcaataaaac aatcattgat gtgataatta attttgcaaa
     6721 cttacaccta ttcataaaat ctactccttc aaattataaa atttataata gcattaaaac
     6781 tctaacttca ctatttatat taattataga acgtttattg atatatttaa acaggattta
     6841 caacatattt aaaagcaatt aagaaacata ctattacaaa tatagaaaat aaagctttta
     6901 cataattttt atttctaaat gcaccaaatg acaccaatcc ttgttgaata gctactgtaa
     6961 ttactaaaag tatcattatt atattaagtt gaaccagttt aaataagtaa agcaatgaaa
     7021 acactactag aagtacacat aatacaatac tagatttatt aataatatca tcaatcttat
     7081 ctaccttctt agattgctta ttaactttat ttaatttttc tttatttaaa ttctcaactt
     7141 ttttattata cctttctctt gttttatctt tatatttagt atttttattt ttatttccca
     7201 tttattttcc atcctctata aattttttaa ttaagcctat acattattta aatattatat
     7261 atttaagata ccatatattc ctacaaaaag gtaacttact atgtatctat ttttgatttt
     7321 cgcataaaaa agccatctcc tttagaatta attctgtatt gagacagcta atttaactat
     7381 ttatcctttg aaacattttc tacattatca tatttactat caactaaaac tatatcaact
     7441 cttctatttt ttgacctacc ttcttcagat gaattttctg ctattggtct aaactcacca
     7501 tatcctacag cagataattt atctggtgct atacctgcat tgtcaataag aagttgaaca
     7561 acattggttg ctctcataac tgataaatcc caatttgact taaactctga attttttata
     7621 gacatattgt ctgtatggcc ttctactctc atgtaactat tcatttcatt taacatctta
     7681 cctatttgaa taatcttatc tctagaatta tccttcacaa tcgctttacc tgtatcaaat
     7741 agtattgtat ctttaaggct tattactaat cctctatctt gaactcttaa tgatactgaa
     7801 ttagccatac cattttcgtt taaatacttc tggatttctt caccaacttt tttaaactta
     7861 gcattttgta aagtatctaa atcattactt cctggttcaa ctactggttc tattttgcta
     7921 ttaccacctt caataactcc agaagaacca ccaaaagcag aatttagaga ttgagataat
     7981 tgtttatatt tctctgcatc aacattactc atagaataca ttattacaaa gaaaatcata
     8041 agtaatgtta tgaggtctga ataagttaaa agccatcgct cattgttttc ttctttttca
     8101 tcttcatcat acaacataaa cattccatcc ttttatatta ttcagctcta ccaccaattt
     8161 cttcaagttc aattagttct tttgaattta aaaatatttt taatttttct gtcattaaat
     8221 tagaattatc accctcaagt atggataata ttgcttctat caccaaaagt ctttcactta
     8281 cttcttgttg atttatagtc ttaagttttg atgctattgg taaccatatt aaattggcag
     8341 aaccaacacc atacaaagta gccaaaaatg caacagaaat ttgaggtcct aatgcatctg
     8401 gattacttga aagattacct aaaatatgta ctaatcccat aactgtacct attatcccca
     8461 gtgtaggtgc atatccacct gctgaatcaa aaattgctat tccttcttta tgtcgttttg
     8521 atgttatctg tatctgtgat tccaatatat ttcttatagt aactgcatcc actccatcta
     8581 ccataagttc aagccctttt ctaataaaag ggtctaattc atcattctca cttatttctt
     8641 tgtcaatact aagaagcccc tctttcctag cctttgttgc aacttctttg aaatagatta
     8701 tattttctat gttattaggc tttttatttt taaaagctaa taacattatc tttatagact
     8761 ttttaaacac agggaatgga gtcgaagctc caacagctcc tatagttcca ccaaacacaa
     8821 caagtgctga tgaaaataca aataatgctc caacatgtcc cccatcaata acaaaagcaa
     8881 ctaccaaaga tgctatcccc actatgaaaa aaattaaagt tgtcatctat tccctccttt
     8941 ctttagttat acatatctaa tcatttatga cattataaaa agactatctt taataaaaat
     9001 agcctttaat aattggcgga gaa
//
LOCUS       DAFKMX010000163         8970 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFKMX010000163 DAFKMX010000000
VERSION     DAFKMX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.164"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            151..642
                     /locus_tag="LZB08_002998"
     CDS             151..642
                     /locus_tag="LZB08_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316480.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYTGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSF
                     KIE"
     gene            670..1617
                     /locus_tag="LZB08_002999"
     CDS             670..1617
                     /locus_tag="LZB08_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316481.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQKGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRNILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            1907..2449
                     /locus_tag="LZB08_003000"
     CDS             1907..2449
                     /locus_tag="LZB08_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7316482.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            2777..4252
                     /locus_tag="LZB08_003001"
     CDS             2777..4252
                     /locus_tag="LZB08_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7316483.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATINEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVVIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGITNDDIGV
                     IEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            4326..4559
                     /locus_tag="LZB08_003002"
     CDS             4326..4559
                     /locus_tag="LZB08_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316484.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            4715..5479
                     /locus_tag="LZB08_003003"
     CDS             4715..5479
                     /locus_tag="LZB08_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7316485.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(5764..8943)
                     /locus_tag="LZB08_003004"
     CDS             complement(5764..8943)
                     /locus_tag="LZB08_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7316486.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMKLENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLKLVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
BASE COUNT         3510 a          936 c         1282 g         3242 t
ORIGIN      
        1 acttttactt gtaaattgaa attaatgaag caaaaaagga ttctttctta aaagtaaata
       61 acaactagat agtgaataat aaaaaatggt aatttgaaat aatttttatg aatagtatta
      121 atatatagat acagggagat tgatgcgatt atgtatgaaa ataataaggt aatatacaac
      181 cttgaaagat gtgaaaaact agaaaagact ctttatttat ctcaatttaa tacatataaa
      241 aattttaaat tagcttctaa aaaaactgta ataaattcat tagagacata tgtttacaat
      301 atgaatatta acaatataag tcttgttgat actatagaat gtatatcatt agaagatgaa
      361 caacttacag gaaagaagtt gattttagtt ggtgatatag atattgatgc aatattagat
      421 tatacaggca ataaaagaaa tagaaattct agtaaaaaaa atacatttaa attaaaaata
      481 ccctttagta catttataca aatgcctaga aagatagaaa ataaagataa aattaattta
      541 aagtatctta tacaagatat tacttcatct attttagatg ataatttatt tattagtgtt
      601 actgcaatta taagttatga aaatagtttc aagatagagt aatctttaaa ttatagaaag
      661 gtggatgcaa tgagaataac gaaaactact tttaatgatg tattttcaaa tgatgcaaat
      721 ggaagtatta taaaagtaga aaaatatgtt gataaaaaat gtgcatctgt atttgagatt
      781 ttgacctaca ctattattgt tacaaataca agtaggtaca aaactggaaa tatatttttc
      841 aaagattata tttctaaata tatagagttt ataaataata ctgtaaaagt taatggcatt
      901 ataaaacgtg gattagaccc acagaaaggt ttttacatag ggagaataga tgcaagttgt
      961 aaaaaaataa tatcttttaa gagtgtagtt ttaccaaatt ctgcacatag gattataaag
     1021 aatagtgcta atatatacta ttattataag tgtaatctag ataaattccc aacaagaata
     1081 tcaatagaaa gtaatagagt ttatactaat gtaaataaaa ttgtattcaa acaattgaat
     1141 ataagtaata ctttaaaagc tcctaaaaat ctaagaaata tattaaaagt aaatgtaaat
     1201 agtaaaatat ttgatgttaa acctatgaaa aatcttataa acaaaggtag taatttgaaa
     1261 ttatgtaact tgatagtttt tgggtgtata gaagttgaaa tagactatag ttgtaaaaat
     1321 aggagtaact ttaaaacagt aaataagtct aatgacatta aaaaagataa gtattataaa
     1381 aatgatatac aaaataacaa tgaaaatata aacgattgta aaaagcaaat aagttttggt
     1441 gataataagg taaaaaaaat aacaaaaact tttggatttt cttgtttcat ttgttctcca
     1501 gttggaatta tttatgaaga tatgaaaaat ataaatataa aaattgaaca tacatcaata
     1561 aatgaattaa atccagaaga attatttgta aatacatcac ttttattgta ttattaagta
     1621 ttttaaagct aaaacttaaa cttattaaaa tagcatttgg ttaaaggaaa atagatattg
     1681 atatatttat catatatata aatgattaat taaacaatta taaataataa gtatattata
     1741 atatttaaaa aatatagatt tatgataaaa taagacaaat atgttaaaaa aatgataatc
     1801 acggagaact tttaccttct tcaattaata tattattatt agtaataact ttaaaattat
     1861 attactatta tataaaaatt ggcttaaaag aaggtgaaaa aagtaaatga aaatatgcat
     1921 aacagtaggt catagtatac taaaaggtgg taaaagtaca ggtgtaaatg gtatagttga
     1981 tgaatacaat tataataaaa aattagctcc tatgttagct gagatgctta taagtcaagg
     2041 taatacagta gatgtaataa tttgtccaga aaggcacttt atgtcagaaa gagaagaatt
     2101 tttctataga gttccaaaag taaacagtgg taaatacgat ttattagtag agctacattt
     2161 aaataaagct gatggaacac aatgtggtac agaagtgctt tactatggca ctgaaggatt
     2221 agaatatgct aaaagagtat ctaatagact tggagaatta tttgaaaata gaggagctaa
     2281 aaaaagagaa aatttatata tattgagaaa tacaaatcca gttgcaatac agattgaaag
     2341 ttttttctgt gataatgtga atgattgtaa caaagcaaat gaatcaggtt atgattatat
     2401 agcacgattg attacagaag gtatattgaa taaagatata aatatgtgag attatggtga
     2461 tttaggtatt gattaatacc ttttaatatt agatataaat aaaaatacaa tgtattagta
     2521 taatttaaac tttcaaaaga tagattagtt tgattattga caagagatac ctccttgaaa
     2581 atattgattt atcagtatat ttaaggaggt atttaaatat aaaaatttaa ttaatttgct
     2641 actaaactat aggtttatag agtatatatt tttatcttca gtctaataaa taatataaaa
     2701 atagtagaac cttgtattat aataatataa tgtagaaaaa tatatagata agataaatat
     2761 aataaggtgg taatatatga aatatacatt tgaaaaattt aagttaaatg aaaagatact
     2821 taagtcattg aagagtttag gatataatat accttctaga gtacaaagag aagttattcc
     2881 aaaattatta aaaggtcaaa acttagtagt taggtcaaaa acaggaagtg gaaagacagc
     2941 aagttttgca atacctttat gcgagaatat aaatgtagat tacaataata ttcaagctct
     3001 tatagtggtt ccaacaagag aattagcctt gcaagtaaaa gatgagatat cagatatagg
     3061 gagactaaag aaggtgagat gtagtgctat atttggcaaa caatctataa aagaccaaat
     3121 agctgaattg aaacaaaggg ttcatattgt tgtagctact ccaggcagaa tacttgacca
     3181 tataaataga ggttctatta agttggaaaa tgtaaagtat ttagtaatag atgaggcaga
     3241 taaaatgttt aataaaggtt ttgttgaaca aatggaaaaa atattattaa atttacctaa
     3301 ggagaaaata gtaagtcttt tttcagctac aataaatgaa gagataaaat atatatgtga
     3361 aaaatatatg ctagattaca gtgttataaa tatagaagaa aatgagagcg atactaatca
     3421 aaaaacaaga caaatagatg acaaaataat caaggcaaat ggaagagaga agtacatcct
     3481 tttaaaagaa ttaatataca gcgaaaatcc aaagagtgtg gtaatatttt gtaataccaa
     3541 agagaaagta tctaaattat ataataagat gagtaaagag ggttttttaa taagagagtt
     3601 acatgcagat ttatcacaag aaagaagaat ttttgtaatt aaggatttta aaaaccaaaa
     3661 atttaatgta ttagttagta gtgatgtagc gtctagagga atacatattg atgatatttc
     3721 tttagtaata aattatgatg taccacaaga taaagaaaac tatatacata gaataggtag
     3781 aactgggaga aaaggaaata gtggaaaggc aattactata gtaactgaaa aggatgaaaa
     3841 atatatagag aatatagaaa cgtatatagg atataaaata aatgaattaa aagatataga
     3901 aaaaagtaga attacacatg gcaaaatttt gtttgaagaa tattcaaaag gaatcctaaa
     3961 aaaagttgac aaaaattctt acaaaagtaa agatataaaa ctaaatatag aaagtgaagt
     4021 tgttaaatta tatttaaatg ctggtaagaa gaagaaaata agagttttag atatagttgg
     4081 tgcatttagt aatataaaag gtataactaa tgatgatata ggtgtaattg aagttcaaga
     4141 tttatgttca tatgtagata tattaaatta taagggagat ttgatattaa aaaaatataa
     4201 ggagatacct ataaagaaaa aaatggttaa agtgaaaaga gatatacgat aatttagtca
     4261 atattgatga attgctttgt tatcgaaatg aagttgcaaa aaacacaata gaagttgtta
     4321 aggatttgaa agctgaagat atgaaacgaa aggtctctcc aattgactta gaaaggattt
     4381 tggtgactgg aggagtaata aagcatgaag attctatttg gttgttggac ttttggggga
     4441 ataaagatat tgctggaatt ttgcttatgc ctcctactcg tcatgttata cttcatttga
     4501 atgattgttg taaatggaag gaatattttc gtacaaaaaa gaaattttac agaagttaaa
     4561 ttataataaa ttagacaaac aatagataaa ttagcaagga atgttgattt tatctacatt
     4621 ctaagttata ctatttttaa gtggctaagg gaatagtcat tttaaatata agtaataaat
     4681 atatacctat agggatatta aaggggggaa atatatggca tattttaact ataacgacaa
     4741 acagtgctac tataatgaaa ttggggatgg tacaccatta ctttttttac atggaaatac
     4801 agcctcttcc aagatgttta atgatataat caatttttat aaagatgaat ataaagtaat
     4861 attaattgat tttgtaggtc atggaaaatc tcaaatggtc gataaatttt ctgctgactt
     4921 atggtttgat gaggctatgc aagtgatatg ttttttagaa gcaatgaact ataaaaaagt
     4981 aaacataatt ggtagtagtg gaggagctct tgttgcatta aatgttgcac ttgagagacc
     5041 tgatttagtg aataaggtaa ttgcagatag ttttgaagga gaagttcctc tagagtcatt
     5101 tgtacaaaat gtgaaaatag agcgtgaagc atctaagcaa gatgatggtg caaaagcttt
     5161 ttatatttat aatcaaggag aaaactggga aaaagttgta gataatgata cagaagctat
     5221 ttttgaacat tataaaacta taggaaagtt ttttcataag cctctagaaa ctatgcaacc
     5281 agaggtgctt ttaacaggaa gtagagaaga tgagtttgta tctctaatat ctagtgattt
     5341 ttttgaaaat actttttcat tacttcttga aaagattaaa aatggtaaaa tgtatttgtt
     5401 tgataaaggt ggtcatcctg caattttatc gaatggatta gatttttcaa atgtagcaaa
     5461 gaaattttta gaggaataat ttaaccaaaa tttacaataa tgaaaactgt aaaatggatt
     5521 gcaaaactta caaaagtgag tgtacatata cttcattatt atgatgaaat agtattgtta
     5581 aaacaaaata ttctaatagt caagttatta catttgtata tgcaatatgt atagaaagag
     5641 atatcattag attgtaaagt tgggattaaa taacaatatt aaattttatt tattcagata
     5701 ttttagctta actatataaa tgttagaaca aatttatata gttaagctgt aggattaaat
     5761 aatttagtta aataagttta ttatctcctc atcagataga ctttttaata caccttcatt
     5821 agaaagttct ccatttataa attgatttat aagctctttt ttactttctt gtaattttat
     5881 tattttttct tctatagtcc ctttagcaat caattttata acttgtactg aatttttttg
     5941 accaaatcta tgagctctat cactagcttg attttctact gatggattcc accatgggtc
     6001 aaagtgaatt accatgtcag cagatgtaag atttaaacca gttccgcctg cttttaaaga
     6061 tattaaaaaa acttttttat ccatactatt attgaactca tctacaagtt taagtctttc
     6121 tttggcatta gttttaccat ctatataatg gtatttaatt ttatacttat caagttcctt
     6181 acttatgttc ttaagcacag aagtaaattg tgaaaacaat aatattttgt ggttttcgtt
     6241 tatactatcc ctaagtattt ctaaacatgt ttcaattttt gaactttttt tattgtaatc
     6301 ttttactact atacttgggt ctaaacaaag ttgtcttagt ttagttaaat aagaaaatat
     6361 aactatttta tctttcttta gatttttatc tttcatttta tcttgtatat cttttgaata
     6421 tacactatat atctttttct gctctttatt caattcaaca aaaaagtttt tttcaatctt
     6481 atctggaagt tctttcataa cttgtttttt acttcttcta agaataaaag gttttatcaa
     6541 ttttttcagg tttttaacat tatcttcttt atttataaac aattcctgaa actttgcttt
     6601 actatataga tagccaggca ttataaaatc aaatatagac cataattcta gtagattatt
     6661 ttccataggt gtaccagtga gtgcaaattt attttctgca tttatacttt ttacagactc
     6721 agttactaaa gctactggat tttttatatt ttgagcttcg tctaatatac aataatcaaa
     6781 ctttatttca ctatatttgt ctaaatcatt tctaagagta ccatatgttg taagtattac
     6841 atcaaaattt tctaatttca taaaacattt ttctctatct cttttatttc catgtatcaa
     6901 taaaactttt atatctggag caaacttttc aaattcattt ttccaattat aaatgagtga
     6961 tgtcggtgta acaataagac tttttttatt agataatgat aataaaaatg caatagtctg
     7021 tattgtttta ccaagaccca tttcatcagc taatatacca ccaaatttat aataatctaa
     7081 aactttaaac caattgaggc catttatttg atagtcacga aggtttgcat ttaaatttgc
     7141 gggaatttct aaatttatgc tatcaaaatt tctaaatttt tcacatatac tgtctagttc
     7201 ttttttacca tctataaact ttaaattttt actacgtatt acatcattta tatatagagc
     7261 tttattttta tgaattttat tattttttga tttatccatt atatctagat tttcaactaa
     7321 ttcaaaaaaa tcttttgttt ctctttctga taaatctata aagtttccat ctttaagttt
     7381 ataaaaagta cgtttttctt gaaaagcttt caaaatatcc ttgtactctt ttggatttat
     7441 atcagatata ttaaaattaa attctaggta attctggtta gtctgattga ttgaagcttg
     7501 aattgatgaa gcgttatata tttttttcaa tttaaacttc tcagaataat atatatctcc
     7561 aaattccttt agagattcta attgtaaatc taaaaatgta aataggtcat ggtcagttcc
     7621 tttaaacaca taatgattat tttcctttgt aaattgtgaa gaagtaagtt tattttcaat
     7681 agtggcttcc ttttgtatgt tggggagcac aaatttttta tcttcatcat catatatata
     7741 ctttacatca caacatatgt tgccatcaat catatcaaag tagaactcag atttaaaatc
     7801 ttttgttata ttatttttta tgttatcatc taaatgaact tcttgagata tagattttaa
     7861 tctaggaact agcaatccta aaacatcctt agaatcctct ttatcaaatt ttaactcttt
     7921 agtctttgat aatatttgat atatttttga ataatcatga gcttttttat gtgataataa
     7981 atagatattg ccatcatata aaagtacatc atttttttta gtaagtggaa caggtagtga
     8041 atctttagaa tataagacta ttttatcttc tatattttta aaatcaaacc ttacaggtag
     8101 agtatcattt aatatctttg gatgatattc ctctttagaa aaaataaggg taaaatcctt
     8161 gtacttaata gcatttaaaa attgttttaa agagcttgca tttatctcca taaatttttt
     8221 tgaatgtgat gaagaaaagt tgtatgaaag atttgtccca tattcttcta taatatctac
     8281 taacttctca tcttcatcag caaaataatg agtgttgggg ttgtaagtaa atttcttccc
     8341 aaaatctaat tcatatccat taagttttga atatgcaaat tcagtaatgt tttttaaaat
     8401 gtacatttta tcaattccta ttttaaattc agcaattgct ttatttgaat ccacatttat
     8461 caatacatct aaattaaccc tttctctttt agtattgtct aacaaagaaa gtaattgctt
     8521 gcttaaatct aagctggttt tagaatttgt attagaaagc ttatccttat ctgcacctaa
     8581 tatatctatt agaataggag gaacatcttt tttatttttt atttgtttta agctttttat
     8641 cacaatagca actatatgct tacatataaa aatacttttg ccctttgatt gacagtcagc
     8701 gcaactacaa tcattttcaa ttatcttaaa gtgctttata tctatagtta ttgaattaaa
     8761 ataaaagcta taattatctt cagaattgac tccaccacga aatgtcaaca ggttattttg
     8821 taggtcatca atatctatgt ctatataatc aactaattga ttagtataat attttacacc
     8881 tcttgaccat cttgatggtt ctgtatttct tcttattagt ttatatatca tatctatttt
     8941 catattattc accttacctc actgtatata
//
LOCUS       DAFKMX010000164         8868 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFKMX010000164 DAFKMX010000000
VERSION     DAFKMX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.165"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            256..948
                     /locus_tag="LZB08_003005"
     CDS             256..948
                     /locus_tag="LZB08_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316487.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1144..3204
                     /locus_tag="LZB08_003006"
     CDS             1144..3204
                     /locus_tag="LZB08_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316488.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3410..3733)
                     /locus_tag="LZB08_003007"
     CDS             complement(3410..3733)
                     /locus_tag="LZB08_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBM7316489.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3726..4427)
                     /locus_tag="LZB08_003008"
     CDS             complement(3726..4427)
                     /locus_tag="LZB08_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBM7316490.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4705..6090)
                     /locus_tag="LZB08_003009"
     CDS             complement(4705..6090)
                     /locus_tag="LZB08_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7316491.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6198..7436)
                     /locus_tag="LZB08_003010"
     CDS             complement(6198..7436)
                     /locus_tag="LZB08_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBM7316492.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(7449..7817)
                     /locus_tag="LZB08_003011"
     CDS             complement(7449..7817)
                     /locus_tag="LZB08_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7316493.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(8345..8767)
                     /locus_tag="LZB08_003012"
     CDS             complement(8345..8767)
                     /locus_tag="LZB08_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBM7316494.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         3350 a         1029 c         1179 g         3310 t
ORIGIN      
        1 aaatgaaagt aatctgaaaa caacataatt caaatcaatt tattcaaata aacctcaatt
       61 tatcttaaat cttaagaaat atttaagaat taggataata ttttcttaag atttatattt
      121 tattatatag ttataaggga aagttacaaa aatgtaagcc aaagtaatat aacaatttag
      181 tcattgttga gaaagggagt gaaagatatt gtataatata gataagtatt attatacaat
      241 agaggaagtg aaaatatgta caatatatta gtagtagacg atgataaaga aattgttgat
      301 tcgatagaaa tatatcttaa atcagaagga tttaaaatat ataaagcata tgatggaatg
      361 aaagctttgg aaataataac agagaatgac attcatctaa tactaatgga tataatgatg
      421 cctaaattag atggaatcaa agcaactgta aaaatacgag aagaaagaaa tattccaata
      481 attttaattt cagcaaaaag tgaagataca gataaaataa taggattaaa tattggagct
      541 gatgattata taacaaagcc atttaatcta ttagagctta tagctagggt taaatcaaac
      601 ttaagacgat atgtaacttt aggtaattat gaagatacat gtaaagaaat tctaaaaagt
      661 ggagctttgg agttaaatac actaacaaaa gaagttaaaa ttgatggtga aaacataaaa
      721 atgactccaa ttgagtataa aataattgaa cttcttttag aaaataaagg acgagttttc
      781 tcaatagatg agatatacga aaaagtatgg aatgaggaaa gttttaattc agaaaataca
      841 gtagcagtac atatcagacg aataagagaa aagattgaaa taaatccaaa agaaccaaga
      901 tttttaaagg tggtgtgggg agttggttat aagattgaaa aattttagtg gaaaaattat
      961 aaaagaaaag agtttaatat ttaattgcat tttgatttta gtcattttta catttacaca
     1021 aatgttagga attttaatat gttttaattt tttctctgag agtttaattt actctatggg
     1081 gattatgctt ttcttaaact ttattttata taaaatactg acaatgtaga tgaggtgggc
     1141 aacttggaga aatatagagt attaaagtta gtttcattaa tcataatatc tatctcattt
     1201 ccattaacta tattgttggt gacaatagga cttgctgagc ctaaatacaa tatacaaaaa
     1261 acttctacaa agagtgggat ttttgatata tctgatgatt tttatgatag taatatattt
     1321 gacgaaggaa caatggcaag tctattaccc aagtttaagc tatctgtagg tggtaaatta
     1381 actagtaaag aaaaaggtaa attccaacaa gaaggtaaaa gtatagattt aagtagtagt
     1441 gattctgagt atgataaaat catgattaat gaacaagctt ctgaagaata tttaagttat
     1501 ctaaaaaata taaaagtgta tgttattaat acatacacag gagtatttta tacaaatacg
     1561 tcatatgcta gtttatatga ttttaaacaa aaaactaaga gctattgtaa tgtagagata
     1621 ataaataaga taggaaagag ctcatatatt aaaacaataa atggagaaaa atttgaactg
     1681 aataatctga gtctggaact aaatgaagta gatgaaggtt ttgaggcata tgtatctttt
     1741 ccagaagagc caactataga agatggaata gtatacacta attttcaaat atttaaacaa
     1801 gctacagaaa aagtaagatt attttttgta gctagtttaa gtttagcaat tatatgttta
     1861 ttatctataa ttttatatat caaaataaaa ggtgaagttt taaaaagaaa taatgtattt
     1921 ttactaatat acagtaagat acctatggaa tttaatgtaa tattagtatc tatttcaatt
     1981 gcaattttaa ctatatatgc tcttgctgta tcttatcata ttgatgtaat tgcattagtt
     2041 tctttatctt tactgatagt atcaaataat atatttttta taaatgtgca attgagctat
     2101 ttagaaaata aaatgtgtat atttaagaca agtattatag taagagtgat tttatggtta
     2161 ataaaaactg taagggaggt cagtaacgct ataaagaaag tatctttagc aaggaaagtt
     2221 attgttatag tgataatatc tttagcttta atcatggcaa ttagcactcg aagtataggt
     2281 atgggattac tgttttctat atgtattttt ttaggattta caatatatat aactaaaaga
     2341 ctttcatatc ttagttacgt aatagaaggt acagaaagaa taaaaaaggg agaattagat
     2401 tataagataa aaattactgg gaatgataat tttacgtctt tagctgaaaa tataaataat
     2461 attggagaag gtcttgatag agctatagaa gagcaagtaa aaagtgaaag aatgaagtct
     2521 gaccttataa caaatgtatc tcatgatttg aaaactccac ttacttctat aataaattat
     2581 gtagatttaa ttaaaaaaga agaatcaata caaccagagt atataaatga ttatataaat
     2641 gtacttgagt caaagtcaaa aagactaaaa ttattgatag aagatttatt tgaagcgagt
     2701 agagtaagta gtggcaatat agaattacaa attagcaaga ttgatttagt gcaactatta
     2761 agacaaagta taggtgaact tgaagaaaaa ttatctaaaa ataatttata tttaaaatta
     2821 aatgttccaa atgataaggt gtatatatgg gcagatggaa gaagaatgta tagagtattt
     2881 gaaaatcttt tatctaatat agcaaaatat tccttagaag aaactagagt ctatattgat
     2941 gtatatgact atggtgaaaa tgtaaaggta actatgaaaa atatatcttc ttatgaatta
     3001 aattttgacc caagtgagat tatggaaaga tttaaaagag ctgatgaatc aaggaatact
     3061 gaaggaagtg gattagggct tgctattgca agagatttag tagctttaca aggtggaaaa
     3121 ttttatatag aaatagatgg agatttattt aaagcaaatt tagagtttca aaaatatgaa
     3181 gaagaaataa caaatatgga atgagatagt atttaagtat atatattttt tttactgtcg
     3241 aaagctttta ttataatata ttagaatatt tacagtagag aatgaagagt aaaaacagag
     3301 taactttaag tcactctgtt ttttatttaa aatcaataat ataacaactt aaattaaaat
     3361 tgtacacata gttttaaaat aacttataca ataaaacatt atccaaatat cacaagataa
     3421 ttccaaataa atatatagat atcactcctg taataataga aataccaata gatttaaact
     3481 tatatgcaac aagaagtaca atcataaaag atataatttt agggttagct atagataaat
     3541 atagactatc tccattaaaa aatacatctt tagcagttaa ggaagtaagt attgctacag
     3601 gtataaattt catgattttt ttcaaaaaat caggcatctc tttttttgaa aaaattaaca
     3661 taggaagcac tcttggtaga tatgtaacaa tgtacattcc aattataata aaaaatatat
     3721 aattattatt cataagattc ctccttttta cagtaagatt cttctataaa gaaaccaatt
     3781 aaagatgcta aaattgatgc aactattatt actaaattgc ttttgaataa tgcatataaa
     3841 ataacagaaa taatgattga tgatataaat actacaatat aaatcttatt ttttatttga
     3901 gaaactatta aagctataaa catagaagtc aatatgaagc cagaaataga actgtctata
     3961 gatatacttt ctcctaaaaa tgctccaata aaattagcta aaactgatga aattaagcag
     4021 aagaaattaa gataaatagc atttctatca gaccaatttc cagcagtaaa tttttcataa
     4081 ttaactgcaa aagtttcatc tgttatagta tgacaaaatg gcaatataaa ttttggactt
     4141 ttatttttta tgtacatgtt caaattagaa ctcattagaa aatgtcttaa gttaactata
     4201 aatgtagtca aaacaataga tgttacactt gcaccagtag aaatcattga agctgcaatg
     4261 aattgagaac ttcctccaaa tactaaagtt accattagtg ctatttgaaa aggtgaaaga
     4321 ccagattttt gtaatagtat tcctcctact atacctatag gtatataacc taagaatata
     4381 ggaattgaag caataaagct ttcttttaat tgttgagttc tttccacaat aaatcactcc
     4441 ttttgtatta aatgtattaa agtattcgta aaacagaaaa atagattaat tgtataattg
     4501 caattataat taaaaataaa tttggattaa tatataaaat actatacaac aaatagataa
     4561 tcttaaacgg ttattaaatt ttatctattt gttattatga tgtaataata catattaatc
     4621 tctataagtt aattactatc tggtgtaatt ttattttaac aacaaaaaaa aatacacaca
     4681 attaaaatat tgtgtgcaat acaattattt tttctcttct aatagtcttt ttaatacgct
     4741 caaaccagct ttaagttctt ctgtactttc tgtagcacat acagctattc ttattgcaga
     4801 aattggtttt tcttttccaa cagcaaaacg ttcagaagca tagacctgta caccttcttt
     4861 aagtgcttgt atctcaaatt tttctgccat tattccatca ggtagaatca accatctaaa
     4921 tatacattct tcatttccca aaagattata tccagataaa tattgattaa taatcttgtt
     4981 tcttcttctt gcagatttac ggcgtgcatt aattaattta tcggcttcac ctgatgtaat
     5041 catgcgataa gcaatttctg ttaaaaaata agattgagat agattaatgt tgtataatgc
     5101 gcttgataat gcctctcgat actgtttagg tgtagagata tatgctagcc gtaaactagg
     5161 tgcaataatt tttgataaac tcgtaatata aattgtttga ttgggcagat aggaagccac
     5221 tggatttaaa tgtgtttcat taagtaaact atgaatagca tcctcaataa caattaaatt
     5281 gtatttagat gcaatatttg caatcatttt tcgtccattt tgtgacatta tatgtgtagt
     5341 tggattttga taatctggta ttatgtatag acctttaata ttttcatttt tacaagcata
     5401 taatagtcct tcttcactaa tctcattatc ttcttgttta attggaatta gttgaatccc
     5461 aagcatttta gctgctgtct ttattccagg ataagttaat gggtctacac ctattctatc
     5521 tccatgttga aacaaaccag ctaaaatagc aacaattgca ttttgtccac cacttgcagg
     5581 aagtaagcta tctgatgtag tttcaagccc agctttataa attaatttgg caatagcttc
     5641 tttttgccat ttagcaccat ctctttgtcc atattggaat atggttttaa aacctatttc
     5701 cttaattatg tgtttaaaca aagtattcat ttcttctagc gtgaaatctg gattcattgt
     5761 gcccatttca ataaatgtaa ttttattatt actggacatc aagaatagat ttgaacgtgt
     5821 atcataagat acaaaagttc cacttcctgt aactccactt attagacctt ttttttcaca
     5881 aattttaaaa gctctagaga tagtactcac attaacatct aagaaatctg ccaattcacg
     5941 ttggggtggt aatttagtac ctggtaataa aaatccattg ttgatatctt gttctagttg
     6001 ttctgcaagc gccttatata atatttgccc ttttgaaggt ctctttggtt tccagttcat
     6061 aggataattt tcaaaagaat taattggcat aagttttcac ctcacattat taatttgatt
     6121 ttttctttat ctaaaacaat aaattctaaa gatacaaata aaaactgata ggaaataatt
     6181 ctatcagttt ttgtctttta attgatttca aatcttccta taatttttat accttcttta
     6241 tcataaagag ggattgtttt tttaaatata ttaaaatttc tttttcttgt atataaaaca
     6301 gcttcttctg gtgtctttgg ttgattatca caatcgtaca aatctctttt ttttacgtag
     6361 ccgacttttt tacctctacc tattgtttgt ataagttctg gttcaatgga atttccattt
     6421 gagtctatta tatgtgtacc ataggtttct ccatttttat tataattaat cttaccaata
     6481 gtatattcta catcttcatt ttgtttaaaa ttggagttga tagaaaatat tgaccctatt
     6541 attataaagg ttaataaaaa cttaagacct aaattaattt tctttttatc ttgattaaac
     6601 atagaattta acctcttttt taaaataata ttttttttat ttagaaactg agatacgaat
     6661 gatgaatact tcaaagattt ttgatacttt attgtatcta atattatata tgcatactct
     6721 ttaatttcac tcgtggtaca cttgtcaatc acaatttcat cacaagataa ttcacataat
     6781 tcatgaaaat atttttttaa taagtataaa attggattga accaatatat gatacaaatt
     6841 atactaatta gaagcttaaa taagttatct tttcttttat aatgaattaa ttcatgttta
     6901 aagatgaatc ttaactcttt attattgtac tttttttctg gaattattat tttacagcgt
     6961 gttaaaccta taatggcagg agaataaata cctttaacct tatatatctg aagtttttta
     7021 tttatactta gttcttttgc ttgttcatct aagatactag taatatctat ttctaaaatt
     7081 tcttttttaa aattttctat gtaattataa aatttaaaat ttgttcttaa atagtaaaaa
     7141 gagattgcta taattcctaa tatccagatg tacataaata tatctaaagt aatgccctgg
     7201 tcatatggta tataattatc gatattatgc ttatagatat taatagtagt attattgata
     7261 tttaatatat ttaaatttat tttagatata aacatcattc tgactactat taaaagacat
     7321 ataaaataat taaacttaaa agtatatttg cttatctttt tagtaaataa aatgaaaaaa
     7381 agaattagtg gacttatgta aatattaaat ttaataactt ctataaataa ttccataaaa
     7441 tacgtctcct attttctttt ttttagccat tgttcaagtt cttttatgtc atcatctgat
     7501 atttcatttc cacaacctaa tgatgatata aagctatgaa tagaattttt atgtataagc
     7561 tctaaaaaat cctttgtttc aaattttaga tattcctcat gtttaacttt aatagtgtat
     7621 aaagtatttc taccatcttt ttcaaattct aaaaaacctt tatcacttaa tctagaaagt
     7681 actttaccag tagtaccttt tgtccatcca tgagaaattt ccatataatt agctacttct
     7741 ctggctggta ttttatctat gcctttatcc catatgaaat tcataactaa caactcggat
     7801 tttggtattt ttttcaatat aatcactcac ctttaacttg attatattag acaaatgtct
     7861 aatagtcaat ataatatatc atcatgtata aataatcttt atattatttt atgcttgaga
     7921 actaagtagt atagatttat agataaaaaa gatattgaga gttttatgga agaggggaat
     7981 atattttcta ttagagataa gaatatgaca tatatttttt attgatttgt acaaaaatac
     8041 tatatagtga ataaattaat atcttttcta aattagaaga ggagaatctt attagataaa
     8101 gattatcctc tttattgaaa gtttaaggga tattagagat tattaatttt tctctctttt
     8161 tttaactaaa agcaataata cataaaatta ttattgctaa tgatactctt aatgtggtat
     8221 atagtcatag tgtcacatgt ttagcaatag ttttttcagt aattattgta acgaatagta
     8281 ggactcataa aatacttaga aatatgtttt tagataaatt ttaaatatat aatctaactt
     8341 atatctataa attaacacta ttattctcca attccatttc tagttttttt ataaatttat
     8401 taaattcttc tggttgcctt tttccaaaca atactcttac aagagctgaa ttgatttttc
     8461 caaatatatt cctcttgctc tcactttcaa taaaaataag ttttgtattt tctaagtcaa
     8521 tatgtactaa ttcatatcta gtaatagtac tttctctttt attgcttgtt tttatctcat
     8581 atactctatt tttttcgaaa ttagttattt caaccttaaa tcttttatta tttttttgct
     8641 gtacaaatgt tcctactgct tttttctcat taaaattagg aaaatttagt cttgcagatt
     8701 ttgtaaatat attaaatatg tcttttgcag aatattttaa taaaacttca gttttaaatg
     8761 tattcatatc gttttccctt ctgtatataa atttaatatt tctaagttaa tttttaagtt
     8821 aaaatagtat aatatgccat attaaatcat gttaaaacta tttatata
//
LOCUS       DAFKMX010000165         8705 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFKMX010000165 DAFKMX010000000
VERSION     DAFKMX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.166"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            167..622
                     /locus_tag="LZB08_003013"
     CDS             167..622
                     /locus_tag="LZB08_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBM7316495.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            671..1255
                     /locus_tag="LZB08_003014"
     CDS             671..1255
                     /locus_tag="LZB08_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBM7316496.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1260..1583)
                     /locus_tag="LZB08_003015"
     CDS             complement(1260..1583)
                     /locus_tag="LZB08_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316497.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSN
                     YLENL"
     gene            1735..2253
                     /locus_tag="LZB08_003016"
     CDS             1735..2253
                     /locus_tag="LZB08_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7316498.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2430..3098)
                     /locus_tag="LZB08_003017"
     CDS             complement(2430..3098)
                     /locus_tag="LZB08_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316499.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3213..4628
                     /locus_tag="LZB08_003018"
     CDS             3213..4628
                     /locus_tag="LZB08_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBM7316500.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTILDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKEFSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4851..5309
                     /locus_tag="LZB08_003019"
     CDS             4851..5309
                     /locus_tag="LZB08_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316501.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5306..5890
                     /gene="clpP"
                     /locus_tag="LZB08_003020"
     CDS             5306..5890
                     /gene="clpP"
                     /locus_tag="LZB08_003020"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBM7316502.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6202..7278
                     /locus_tag="LZB08_003021"
     CDS             6202..7278
                     /locus_tag="LZB08_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBM7316503.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7345..>8705
                     /gene="bclA3"
                     /locus_tag="LZB08_003022"
     CDS             7345..>8705
                     /gene="bclA3"
                     /locus_tag="LZB08_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBM7316504.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGNTGADGATGPTGATGATGV
                     AGAT"
BASE COUNT         3329 a         1116 c         1605 g         2655 t
ORIGIN      
        1 tcaacttaaa taattttaag tattatatgt gttttgatta aagaaaacta aaattttaag
       61 gaaaaataac atatttgtat taaattttat tgaaatgatt aaatttattt acttaatgta
      121 aattattagc taaaattgta ttaaataatt aaggaggtga gtagctctgg atgaaataga
      181 tttaaaaata attaattctc taaaaaagaa tagtagggct tcaacctctg aaataagcag
      241 acaaattaat ttatcagtac cagcagtctc agagcgtata aggaaaattg agcagtcaaa
      301 tttaattcaa aagtacacta ttcaaattaa tcgggaaaaa acaaaatata agcttttggc
      361 ttttatattt atcaatactg atttatctaa aagtatgaaa gaatttaaga aaactatatt
      421 ggaatatgat actgtattag aatgtcatca tatttcagga gagtatgatt acttaataaa
      481 agtattagca gaagatacaa gtagtcttga agagtttata tcagtgactt tgaaggaaat
      541 tggggggatt caaaaagcta atacaaccat tgttttatct acaattaaag aagaatttaa
      601 tacaataaaa gatatttttt aaataattta ctagtaaagt gttaattata ttttaggagg
      661 aaaatagaat atgaaagttt tagttttttt agcaaaaggt tttgaaacta tggaatttag
      721 tgtatttgta gatgttatgg gatgggctcg taacgattat gggcatgata ttgatgtagt
      781 gacttgtgga tttaaaaaac aagttatgag tacatttaat atacaagtat tagttgataa
      841 gacgatagaa gaagtatgtg ttgatgatta tgatgctcta gctattcctg gaggatttga
      901 agaatttgga ttttatgatg aagcatatga ttcatctttt ttaaatctaa taagggagtt
      961 taattcaaaa gaaaaaataa tcgcttctat ttgtgtggca gcattgccag ttggaaaaag
     1021 tggtgtttta aaaaatcgta aagctactac atatcatttg aaaaatggta aaagacaaag
     1081 acagttaagt gagtttgatg taaatgtagt taatgaacca attgttgtag ataaaaatat
     1141 tataacctca tattgtccag aaacagctcc acatgtagct tttaagcttt tagaaatgtt
     1201 gacatctaaa gaacaaatgg atgaagttaa attagcaatg ggatttaaac tgtaatggtt
     1261 tatagatttt ctaagtaatt agaaccccag tcacacatgg aatctaaaat tggaataata
     1321 ctctttccaa aatcagatag cgagtattct acttttggag gaacaacagg ataaaccact
     1381 cttataatta aaccatctga ttctaattca cgtaattgtt gagttaacat cttaggcgtt
     1441 gctttaggaa ttaatttttt taattcattg aacctcaata tagtgtcttt aagatgccaa
     1501 agtataagtg tcttatattt accaccaatc aatgcaagtg ttgcttctat tgggcaactg
     1561 taattatcta aacatttttt catttttggg ctcctttatt tattttaaag tatcttttta
     1621 ggtactatat tacaaaaaag tgcattattg taatatagta ccatagtgat aaaattatag
     1681 cataggttaa ataaatcatc aacttagcct aataaaacaa tagggggtaa ataaatgaat
     1741 ttcgttgaac ttgcaaaaaa aagatattct tgccgtaatt atcaagatag aaaggttgaa
     1801 aaagaaaaac ttgagaaggt tttagatgtt gcaagaatag ctccaactgg tggtaatcgt
     1861 caacctcaaa gattgattgt tattcaggaa aaagaaggta taaataaact ttctaaggct
     1921 gccaatatat atgatgctcc attagcaatt cttgtatgtg gagataaaga caaagtttgg
     1981 acaagacctt ttgatgggaa acaacttaca gatattgaca caagtatagt tacagaccat
     2041 atgatgttac aggcaactga acttggattg gcaagtgttt gggtgtgcta ttttaatcca
     2101 gatataatta gagaagagtt tagtttgcca gataatctag aaccaataaa tatattattg
     2161 atgggatatg aatctaagat accagagagc ccagagagac atgaaaaaac tcgtgttcct
     2221 ttaagtgaaa ttgtttctta tgaaactctt taattataaa attaagttat tcattaggat
     2281 aaatgtaata aagtgtttat tataaacaaa attcaaaata aataatatct acatataatt
     2341 aattttagtg aagtatatct agtttaacta gaatgtatac ttcactaatt tatttattca
     2401 aaatatttat gaggatttta atatattaac taaaatatat caattgaatt aacttttaaa
     2461 tctggaaaag cacttgaaat atagtgtttt atgtcattta aatggtcttg aatttgcttt
     2521 tctgatattt tatcaggtcc atatacaaaa tacataatat ttttagtatc ttttgttata
     2581 gctgttcgat tatcagggcc attaagaata ctagataata taccaatatt atctgataaa
     2641 aataaatcat cttttgttaa aatatgttct ttaccaccta taccataaaa atgttcactt
     2701 ccatttgcta attctatttt caaaggtaat tccattttat caaggtcatg acctgctgtt
     2761 agaagaagat ttttcaattc tgctaaaaac attgactcaa ttgttacacc tacatctggg
     2821 ataccttttg attttaatag aatagattct aattgaagca atacagggta agttttcttg
     2881 aattttttat aatactttat ataacttgaa acaggctctg ttttaatttt ttctttacga
     2941 ttgtattctg ggtgttgttc tataatattt ttaattgtag agtttttaag aactaaaaaa
     3001 ttttctttat taggttttga atgtgtaata tttataacca taactccaaa tttaatattg
     3061 ggataaattt ctttaacttt atgtgatatt tctaacataa tatagactcc tttttatttt
     3121 atattttatg atataatctt agcaaattaa aagtggattt tgtagttcca tttttatagt
     3181 aattttatag aaccactttt ggaggtatta gaatgtggat tacgattaat agaaatagta
     3241 atatatcatt atcaagacaa atttattgtg agataaaaga tatgattcta gatggaagct
     3301 taaagtttga gcaaaaactg ccatcatcta ggactttatc aaaagaacta tctgtatctc
     3361 gtaatacaat tttagatgca tataatcaat taattgcaga aggatatttg gaaacaagga
     3421 atggatatgg aacaattgta gctaaaggaa tatcagggca taaagttgat tttaagttaa
     3481 atgatacttt tagagatgaa aaagatttac atactaataa acaaaaaaat tatatagatt
     3541 ttcgttcagg agttccagaa ttaaaactat ttccacgcaa agaattttca aagctatata
     3601 aacaaatact gtatgattta tcagattcgg atttcagata tggaagtaca gctggtgtat
     3661 gggttttaag agtggaaata tctaagtatt tatttcgtac tagaggaatt agatgtaatc
     3721 ccaaaaacat tatgattgtt tctggttcaa cacaaggttt atctttaatt tcacagcttt
     3781 tatacaaaag taataaaaag gttgctgtag aagaccctat tcataaaggc ttattaaagg
     3841 ttatttcatc tattggatat tcagttgttg gaattaaagt agatgataag ggaatgaata
     3901 ccaacttaat aaaatcaata aaagggattt cttttgttta tactacacct tctcatcaat
     3961 acccattagg agggatttta cctattcaaa gaagactaga gcttattaaa tgtgcaacag
     4021 agaatgatta ctatataatt gaagatgatt atgatagtga atttagatat gagggtcaac
     4081 caattagttc tctttatgaa cttagcccaa ataaagtaat ttacataggt tcttttagta
     4141 aaatattatc tccagctatt cgtgttggat ttatgttatt accagacaat attttagaaa
     4201 gctataaaac cttaaaaatg tactctgatg ttcatacaga aacaatattg caacatgtat
     4261 tagctgaatt tattggaaat ggaggtttag agaagtacat atttagaatg aagaagttgt
     4321 acaatagaaa acgtaagtac ctcataaaag ttttaaacac ggaatttaaa aatgaattta
     4381 aaataaaagg tgaagcagca gggcttcata tagttgctca tttctataat gttaaattta
     4441 cggaaagttt aattcataaa atttttgaag caggtataaa agtttatcct atacaacaat
     4501 atgcaattga gaatatatta gaatatgacc atgaaattat actaggttat gctcatctta
     4561 gttttgatga aattgaaaaa ggtgtaaaaa tactcagcaa aataatacat atgaatcaag
     4621 gtacatagat aaaacatata gcaatctaca tataatagat gtaattaagt ttaaacttta
     4681 aaagaaatat atattaatat ttttgtataa aaatacctga tattttttta tcgatacttt
     4741 attgctatac actaaattaa gatagcaatt tatattaaga aaaaactttt tgcataggtt
     4801 ataatactta aaaggagagg atagagttga aaacaaatac agatgttatt atggagataa
     4861 ttgattatat agaagaaaat cttacagaaa atctaaattt agataaaata gcttatattg
     4921 ctaattattc aaaataccat ttacacagaa tgtttacaaa tgttgtagga tgtactatcc
     4981 atcaatacat acagaggagg agacttactg aagcagctag acaacttata tttacagaca
     5041 aatatattat agacatagct attacagtag gatattctac acatcaatct tttacactag
     5101 catttagaaa tttatataaa gaatctccac aagtatatag aaataaagat gaattttatc
     5161 ctattcaaca gagatttgtt ttaaatctaa atcatcaaat tcaaagcgaa aaatgtattt
     5221 cttatgcaaa tatgaattat aaacaaagaa gagatgaaat caattcttat cttaacttta
     5281 atattaattg tgaggtgaaa gtagcatgag tttagtgcca catgtaattg aacaaacagg
     5341 tcaaggggaa aggtcttatg atatatattc aaaattatta aaagatagaa ttatatttat
     5401 aggagaagaa attaatgatg ctattgcaag tttagtagta gctcaattac tgtttttaga
     5461 atcagaagac ccagattcag atataattat atatataaat agccctggtg gctctgctac
     5521 atcaggattt gctatatatg atactatgaa ttatgtcaga tgtgatgtat cgactatttg
     5581 tattggtatg gctgcaagca tgagtgcttt tttacttgca ggaggaacac atggaaaaag
     5641 atgtgcttta cccaattcag agattatgat tcatcagcca atgggaggag ctaaaggtca
     5701 agcaacagat gtaaaaatag ctgtagataa tatattgaaa ataaaagaac gattagataa
     5761 aattctttct gaaaatacag gtaaatcaat agaagatatt aggagagata cagatagaga
     5821 taattttatg actgcactag aggctaaaga gtacgggctg attgattata taatgaatag
     5881 gaatgaataa agtatcaaag tatcatatta aaatgtctgt tttacaagta tatatataat
     5941 attaaataag agtgtctcaa aatgaacttt ttaatttatg ggacactctt ttttgtatga
     6001 aattaaatat ggttttatca tttcaacaat tattaccaaa tttcaagttt aataaattct
     6061 actataaaaa atacataaaa gataaggttc tttagtaact ttctgtcgtc aaaagttttt
     6121 ataaatttaa taacaaaaca agagaaaata ccatatatta cttatgtctg caatatatag
     6181 aagacaaagg agttgtacat tatgattaca ataagtttgt gcatgattgt taagaatgag
     6241 gaagaagtta taggcagatg cttagaatgt gtaaaggata tagtagacga aattataatt
     6301 gtagataccg gttctacaga ttccacaaaa gaaatagtct caaaatatac agacatggtg
     6361 tacgatttcg aatggataga tgatttctct gctgcaagaa atttttcatt ttctaaagct
     6421 tctaaagact atactatgtg gcttgatgca gatgacataa ttttagaagc tgatagagaa
     6481 aaattattaa agattaaaga aagcttggat acttccattg atattgttat ggcaaaatac
     6541 aatgtatcct ttgatgaaaa tggtaaccca actctctcgt attatagaga acgattgttt
     6601 aaaagaagtt ctaatcataa atgggttgac cctattcatg aagtagtacc tctttttgga
     6661 gaggtatttt attctgacat tgcaatatct cataaaaagc ttcatagaca ggaccctcta
     6721 agaaatctta gaatttttga aaaaatgatt tcagaaggaa aaacattgga gccaagacac
     6781 caattctatt attctagaga gttgtactat aatgctaggt ataaagaagc aatagaaggt
     6841 tttactaagt ttttagactc atcaagagga tggattgagg attgtatcag tgcttgcaga
     6901 gatttagcca catgttatta cttaattaat gatgaaaaaa gtgccttata ttcattgttt
     6961 agaagtttcg aatttgatga gccaagagca gaaatttgtt gtgacatagg aaagcatatg
     7021 tttgatagac aaaaatataa agaagccatt ttttggtata aggtagcatt aacaagagat
     7081 aagaatgata ctaatggagg ttttaagtca aatgattgtt atggatatat accatctata
     7141 caattaagtg tctgttatga caggttagga gaatcagata aagctattta ttatcatgaa
     7201 aaaacaaaag aaatcaaacc taacgatagt gctgttttgc acaatgaaaa ttattttagt
     7261 aaatttaaaa atagttgata ggtcacaatc atactaaatt tgacatatta tatatagagt
     7321 ttaaaagctc aaatatatca ggaggtgctt ttaataatga gtagaaataa atattttgga
     7381 ccatttgatg ataatgatta caacaatggc tatgataaat atgatgattg taacaatggt
     7441 cgtgatgatt ataatagctg tgattgccat cattgctgtc caccatcatg tgtaggtcca
     7501 acaggcccaa tgggtccaag aggtagaacc ggcccaacag gtccaacggg tccaacaggt
     7561 ccaggagtag gggcaacagg cccaacagga ccaaccggtc cgactggccc aacaggaaat
     7621 acagggaata caggagcaac aggattaaga ggtccaacag gagcaacagg ggcaacaggc
     7681 ccaacaggag cgacaggagc tatagggttt ggagtaacag gtccaacagg cccaacagga
     7741 gcgacaggag caacaggagc agatggagta acaggtccaa caggtccaac gggagcaaca
     7801 ggagcagatg gaataacagg tccaacagga gcaacagggg caacaggatt tggagtaaca
     7861 ggtccaacag gcccaacagg agcaacagga gtaggagtaa caggagcaac aggattaata
     7921 ggtccaacag gagcaacagg aacacctgga gcaacaggtc caacaggggc aataggagca
     7981 acaggaatag gaataacagg tccaacagga gcaacaggag caacaggggc agatggagca
     8041 acaggagtaa caggcccaac aggcccaaca ggggcaacag gagcagatgg agtaacaggc
     8101 ccaacaggag caacaggagc aacaggaata ggaataacag gcccaacagg tccaacagga
     8161 gcaacaggaa taggaataac aggggcaaca ggattaatag gtccaacagg ggcaacagga
     8221 acacctggag caacaggtcc aacaggagca acaggcccaa caggagtagg agtaacagga
     8281 gcaacaggag caacaggggc aacaggagca gacggagcaa caggagtaac aggtccaaca
     8341 ggagcaacag gggcaacagg agcaaatgga ttagtaggcc caacaggagc aacaggagca
     8401 gcaggaacac ctggagcaac aggtccaaca ggagcaacag gcccaacagg agtaggaata
     8461 acaggagcaa caggggcaac aggagcgaca ggtccaacag gagcagatgg agcaacaggt
     8521 ccaacaggag caacaggaaa tacaggagca gatggagtag caggtccaac aggagcaaca
     8581 ggagcaacag gaaatacagg agcagatgga gcaacaggtc caacaggagc aacaggaaat
     8641 acaggagcag atggagcaac aggtccaaca ggagcaacag gagcaacagg agtggcagga
     8701 gcaac
//
LOCUS       DAFKMX010000166         8633 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFKMX010000166 DAFKMX010000000
VERSION     DAFKMX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.167"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..649
                     /locus_tag="LZB08_003023"
     CDS             <1..649
                     /locus_tag="LZB08_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316505.1"
                     /translation="KEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEIFRI
                     ENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLNFKV
                     LSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDLNRI
                     FNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
     gene            761..1990
                     /locus_tag="LZB08_003024"
     CDS             761..1990
                     /locus_tag="LZB08_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBM7316506.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1998..2672
                     /locus_tag="LZB08_003025"
     CDS             1998..2672
                     /locus_tag="LZB08_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316507.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2675..3907
                     /locus_tag="LZB08_003026"
     CDS             2675..3907
                     /locus_tag="LZB08_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316508.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(4193..5458)
                     /locus_tag="LZB08_003027"
     CDS             complement(4193..5458)
                     /locus_tag="LZB08_003027"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBM7316509.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5511..6659)
                     /locus_tag="LZB08_003028"
     CDS             complement(5511..6659)
                     /locus_tag="LZB08_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBM7316510.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6759..7304)
                     /locus_tag="LZB08_003029"
     CDS             complement(6759..7304)
                     /locus_tag="LZB08_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBM7316511.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7527..8471)
                     /locus_tag="LZB08_003030"
     CDS             complement(7527..8471)
                     /locus_tag="LZB08_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBM7316512.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3016 a         1405 c         1140 g         3072 t
ORIGIN      
        1 caaagagatt ttaaatatgt gggtacatga tgtaaagata cctatagcta taataaaact
       61 cattcttgaa caaaatgaaa atttaattga tgaaaaaatt tcagatgaca tagatagtga
      121 aatatttaga attgaaaact ccgttgaaaa aattttatat ttatcaagac tcgaagattt
      181 tcataaggat tttttaattc aagaggtaaa tattgaaaaa atcattagaa atattataaa
      241 aaaatactca aaatatttta tttcaaaaaa aattatttta gacttaaata atttgaattt
      301 taaggtttta tctgatgaaa aatggctata cttcatattc gagcaattat tgagtaattc
      361 cctaaaatac tcctcattag gagataatat atctatctat tgcaaaagaa ccaaaaatta
      421 tttacaatta agagtggaag atactggttg tggtataaaa aaggaagatt taaatcgaat
      481 attcaataag ggatttacag gtaataatgg aagaaacaat tctaaatcta caggtcttgg
      541 gctttattta gtaaaagaac tttgtgataa gcttggtcat aaaattgatg tagattcaga
      601 gtataaccaa tatacaaaat ttactattac attcaaatgt gatttatagt atcttattat
      661 tgatagttta ttttcattaa cttatattca acttcttata gtaattctat atgtacatcc
      721 cagtatataa ttaagtagtt tttataaaaa ggaggaagac atgtcttttc taaaaaaatt
      781 taaaaataaa agatttagaa ataacagttt taaaagtaac aactttagaa atagcagttt
      841 tggaagtagt ggcttcaagt ctaaaaataa aggttttaaa aacaatccct ttaaaaataa
      901 gagattcaaa atatatgcag gaatagcagt aatcatactt ttaattctag gagttcttgc
      961 ttatgttgat tcgaaaaaca ctgaattaca agctaatgaa aattttatcg aaacatatac
     1021 tattcctgaa aatgaaaaaa tatttataaa tgggatggtt gttcctaagc aaactaagga
     1081 cttcaatata tctggagatt atgagttaag tgatgttaat gttacaaatg gtcaaaaagt
     1141 aaatcaagga gaccttcttt ttactgctaa aaatccaact ataatagcag aaatagatag
     1201 tttaaaatca caacttagtc aatacaaaaa acaaaaaata tcactttctg atattgctga
     1261 aaataaagat gctattgctt ctataaatgc acagataact gctttaaact ctcagatagc
     1321 ttctctggat aaaaaagctt atgacagagt tactgctcct tttgatggca cagtatactt
     1381 gaatgaccaa actggaaatc cagaccaacc agtttcattt atgactattc aaggtctaga
     1441 gttttatatg aaaggacaag caagtgaaca agatttacct aaaatgaaga ttgaccaaat
     1501 ggtcaatata ctcgtttttt caactgacca aaagcttact ggtagaataa gttttatatc
     1561 tgataaacca tctactccaa atactgaaat gggagctcag cagaatacac tatcttatta
     1621 tgatataaac atagcatttg ataatcaaga aggattagta aatggattcc atcttcaagc
     1681 atcacttgaa gtagctaatt caagctttaa aataccagca tcttgtgtac ttaaagacaa
     1741 caaacactca tatgtattta aggatttaga tggtatattg aaaaagcaaa ttgtagatgt
     1801 agcaagtcaa aatgatgatt ttgctgtagt tagaggcggt cttgaacaag gcgatataat
     1861 aataaaacac cctactaaag aaatgaaaga aggagaccca gttcaaggcg atggcgtaac
     1921 tgctggttct aataatggag atactactcc aaacaaaaaa gtagaagaag ttactatgga
     1981 tgtgaattaa tttacatatg ttaattaaat tagaaaatat tcaaaaatat tacaaagtag
     2041 ggaaagatga attacatgtt ttaaaatcct taaatttaga aattgaatct ggagaatttg
     2101 tcatgataat gggtaagtct ggtagtggta aaactacttt acttaatatc ttaggttttc
     2161 tagatgtatt tgatgaagga agatatatat ttgatggaac agatgtaact aatttgagtg
     2221 aaaatgaacg ttctgttttt agaaatatta atattggatt tgttttccaa caatttaatt
     2281 taatagaaac tctaaatgta tatcaaaatg tagagttgcc tctaatttac aataaagcac
     2341 taaaaaaatc taatagagaa gaaattgtta aagataaatt aagttctgtt gggcttttgg
     2401 acaagcttaa gcagaaacca cttcaattat ctggtggtca acaacagcgt gtagccattg
     2461 cacgttgcct agcaaatgac cctcagataa tctttgctga cgaaccaact ggggcattag
     2521 atagtgaaac tagcagagaa attatggagc ttttaacaag gctcaacaaa caaggtaaga
     2581 ctataataat ggttacacat gatcaagatt taactaagta tgcaactaag gttatacgac
     2641 ttaaagatgg tgtgtttact tcggaggtgt aatcatgagt ttattaaaaa attctttggc
     2701 caatttaaaa ggtcacaagc ttcgtgtatt tgtagcttta ctctggatta taataggaat
     2761 aacttcagta atacttgtta gttctatagg taatggattt caaaaagaaa ttaagaaatc
     2821 tgtaaacaat gtaaacccta ataaaactac tatttcgttt gaatcagccg ataacacagg
     2881 tcttactgat gatatgagta tttttttaaa accctttaat gcaaaagatt tagaagaatt
     2941 atcatttgtt gaaggagtag aaagaatagc tccttcaaga gatgggttta accttgattc
     3001 agtttactct tcacaagcat cttttgataa aaaaactacg tatgttgatg ttggaccagt
     3061 aaaaaaagat tctaaaataa atttaatttg tggtagagat ttttctttag atgatgaaaa
     3121 aagaaaagta attttactta cattacaaag tacaagtgaa atttttgaaa atccagaaga
     3181 tgccttaggt catggtatca acataaatgg aactatattt gaaataatag gggtattaga
     3241 tgattctcag caaaaccaag ctggaggatt ctttggaggt taccaagata tgcaatttac
     3301 tacttcccta gtacctaaaa aggcttttga cactttaatg agtcaaaatt cttattcaaa
     3361 tgaaatttac cagttagact tagtttcttc taaaggctat aatgtaaatg aagttgcaaa
     3421 caatgttata gctaaattat atgagatgca ccctggtatc aatggttctt atactacacc
     3481 agacccaact gaacagacag cttatcttga aagcattaat tccaatgtaa acaaatatgt
     3541 atctataata actgtagttg caatgtttgt tggtggtatt ggtgtaatga atataatgta
     3601 tgtatctgtt atggaaagac aaagagaaat tggtataaga cgtgctattg gagcaaaacc
     3661 taggtctata ttatttcaat ttttagttga agctgttttt ataacagtat gtggtggaat
     3721 tttaggaact atagttggat ttgctgctac taactatgtt tctaaatata taggttttga
     3781 agctatacct agcttaaata gcttatttta tgcaattgta gctactatat tgactggagt
     3841 tgtatttggt ctgattccag cctttaaagc ctctaaacta gaccctataa aggcaattta
     3901 taagtagaaa ttgtataaag attttcgatt tttagattag tttatataaa acatacttaa
     3961 atatataacg cactattata tatgatagat taggaactta atctttccaa atataatagt
     4021 gcgttctttc ataaaaagga ttctcttcct tactatatct aaattaccaa gatgttatgt
     4081 tctaattgtt caaactaata cttccaattt tgattaatca atctcattct gctaatatac
     4141 aatcttttac tttcaatcat tgtacactcc gccttaaaaa aataatctta aattaggcgt
     4201 tatatacaat ctccccatta catattgtat aattaacctt tccatataaa gatttacctt
     4261 taaatggtga attatcagct ttagaaacaa agctttcaac tgtccattct tcttctggat
     4321 tgaatataac aatgtctgct acagcccctt cttctataaa tccaatattt aaattgtaca
     4381 actttgcagg atttatagac attttttcta ttaactgcat catactaaga tgatttttat
     4441 gcaccaaatt tgttattcca agagctaaag aagtttcaag cccaataatt ccacttggag
     4501 cttttataaa ctctctatct ttttcttctt tactatgagg tgcatggtcc gttgctatta
     4561 tttcaatagt accatcattt aatccctcta taatcttatc tctgtcccat ttacttctta
     4621 gaggtggatt catttttgca tttgttccaa actccaatac atcttcctca gtcaatgtaa
     4681 agtgatgtgg tgaagcttct gcaactacat ttgcacctaa gtattttgca aatctaacca
     4741 tatcaacaga tacacctgaa cttatatgtt gtatatcaac tttagcacca gtctttagtg
     4801 ccaacataca atctctggct accataacat cttctgctac acttgatgca ccttttaatc
     4861 ctagttctga agctaccttt cctgcattta cacctggatt tccaactaaa gaagggtctt
     4921 cttcatgaaa actaagtggt acatccaact cttttgcctt aatcattgcc tccataatta
     4981 gattgctatc cattaaaggt aatccatcat cagtaaaccc tggtacacca gcttttttaa
     5041 gagcttccat atcaactaac tctttacctt caaaaccttt agttatagct gctgcttgta
     5101 aaacatttat acatgcattt tttgactttt ctttaacata attaaaagta tcttcattat
     5161 ctacaatagg attagtattt gccatacata taacagttgt aaatccacct ctagcagcag
     5221 atttagctcc actttctata tcttctttat atgtaaaccc agggtctcta aagtgtacat
     5281 gtacatccac taaacctgga gcaactacat taccactagc atctattatt ttttcatatt
     5341 catcactttc atcaaacttg cctattttat aaattttatt ttcttttata attaagtcaa
     5401 tattctcatc tctttgactt ttagggtcta ttactctacc gtttttaatt aaaatcatat
     5461 tatttctcct ttatattttt aatttatata gactcatata atattataca ttattttaca
     5521 tacttaaata caaaattacg tttgacatca atagcctttt taggacaaag ttcatggcaa
     5581 cagtaacatc ttatacattt acttaagtct ataatagcac ccttagtccc tatctctata
     5641 gtggatgcag gacaagcttc cttgcatttt ccacatttta tgcaatcttg aaatcttacc
     5701 acaggctttg gtgttataag ctttgaaata ggttcattta aaaattttgg tagctttacg
     5761 gaaaacaatc ttaaatcctt acttacattt ggaatcttgt aattttttat aattaaatct
     5821 tttatatctt cacctaaaat ttcaatatct gaaaaatctt cttgtatata tcctctctta
     5881 atagcacctc taattgtagc aactctattt ggaagtaaac ttataatctt acatgcaaca
     5941 acatctatag catatggatt ttgtgatgct aataaaactc ctatttctcg agtagtacca
     6001 gctgaaggtc catcaccttc catacctact ataccatcca taattgtcaa tgttggtttt
     6061 atataatcac aaatatctaa aagtacattt tcgcaaaaca actcttctgt aggaaatcta
     6121 taatgtatct ctgctttttt caatccagct atgcatccat ataagttttt tgtaccacca
     6181 gtaaaagttg ccattatatg agtctttaat ttacaaagat ttattatata atctgaatct
     6241 actattggtt taataatatc catatatttt agaacatgtg ctttttcact atgtatctta
     6301 acatctgaaa catcataatt taacttaatg cccagttcat ttgctacttc ttcaattcca
     6361 gatgccttat aaatgctctt aagagaactt tgattatatg gaccaccagg gctatctgct
     6421 attgtaatat cacaatttaa ctctaaaagt ttttcacata caacttttac aaccatagga
     6481 tgtgtagttg tagcttcttc aggctttttt ttcattaata aatttggttt tataagtact
     6541 ttgcttcctt ttttaacaaa cttatctata ccaccaatat cctctaaatt tttttcaata
     6601 gataacttta cttcttcata attgtaagtt ttacaactcc taacagatac cgacttcata
     6661 ttatctccat ccttctatca caacagttta tttgtataat ttaaaaaagg agaaactaaa
     6721 gtttctcctt ttaatctatg ttttaattgt tttaattatt attttccaaa atgtctgttt
     6781 aataatccta agaatgcttt accatgtcta gcttcgtctt tgcacatttc atgtactgta
     6841 tcatgtatag catctaatcc taattcttta gctctcttag ctaattttaa ttttccatca
     6901 gttgcaccat actcagcatc aactctaact cttaagtttt cttttgtatc tgcaactaca
     6961 acttctccaa gaagttctgc aaatttagca gcatgttcag cctcttcaaa agctattctc
     7021 ttatacgctt cagctacttc tggataacct tctctatcag cttgtctact cattgctaaa
     7081 tacattccta cttctgtaca ctcaccagta aagttagctc ttaatccttc gattatttct
     7141 gcatctacac cttgagctac tcctattcta tgttcatcag cccaaaccat ttcgcctttc
     7201 atttcttcaa atttatcagc tccaacttta catactggac attgtgctgg tgcagcatct
     7261 ccttcatgta tatatccaca tactgtacaa acaaattttt tcataattaa tttcctccca
     7321 attttatata attaaatttt tatttttaag tttaataact tgctacaaga atatatatac
     7381 cccagacttt atagcttaaa cactaaaaat aaaatttatt tcctaattaa ttttatcact
     7441 ttgaaacacc agtatcaacc aagttttgat gtcaaatttc tacataatac ataatttatt
     7501 tgattaatta ggaaatagtt gtcatattaa atactttcta atgaagactc ctctgaatct
     7561 ttaagtttca cttttttgct ctttaaaaaa gataatttag tttctgcagt tatgattgca
     7621 acaaatatca agatactacc aataaccaat tttgctgtaa taagctcctt taaaattatt
     7681 atagaaaata ttgtaccaaa tacagcctct gtggataata ttatagctgt tctagttcca
     7741 tcaactcttt tttgacatat agtctgaaat aaaaatccaa ttgtagtact aaatacagct
     7801 agatacatag ttcccatgta accagataat tcagcttcca tctttaattg acctgcaaat
     7861 gtttgaacta caacagacat taaaaacgct actgtaaatt gaatagctgt caatgccatt
     7921 gggttgttat cttttgcaaa ctcacttgta aagaatatat gaaatgcaaa tccgaatgaa
     7981 catataagtg ttagaaaatc tccaaaatta atagagaaat cagcttctaa agaaagtatc
     8041 ccaattccta taagagctac caaactactt attattccta ttttatcaag ttttcttcta
     8101 taaagtataa atcctatgaa cggaactatt acaacattcg ctgctgttat aaaagcattt
     8161 ttagatggtg tagtgtacat aagtccaata gtttgtactg tgaatgctag aaataaaaat
     8221 attcctaaaa tccctcctgc ttttaataat tttttaccca tattggcacg aatctgttta
     8281 aaaaacagta aattcataat tatagctgcc aagaaaaatc ttaaagttat gatttgtaga
     8341 ggtgtaagcc caccgtctaa tgcaaattgg gttgctacaa agccacttcc ccatataatt
     8401 gctataaata ttagtcctat ttctccaaag tattttttca ataaagcccc ctccttttta
     8461 tttttttgca aaattataaa ccccctaaac agcaagagag tttttctata agcattacta
     8521 taaattaata tagtaaattt tttcagtatt ttaaatctaa atttacatac attttgagct
     8581 caaatttata tccactattt aacagtaata tattaatcat tttttgtaaa cac
//
LOCUS       DAFKMX010000167         8582 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFKMX010000167 DAFKMX010000000
VERSION     DAFKMX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.168"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            63..218
                     /locus_tag="LZB08_003031"
     CDS             63..218
                     /locus_tag="LZB08_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316513.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            348..1361
                     /gene="aroF"
                     /locus_tag="LZB08_003032"
     CDS             348..1361
                     /gene="aroF"
                     /locus_tag="LZB08_003032"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBM7316514.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1383..2447
                     /locus_tag="LZB08_003033"
     CDS             1383..2447
                     /locus_tag="LZB08_003033"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBM7316515.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2447..3760
                     /gene="aroA"
                     /locus_tag="LZB08_003034"
     CDS             2447..3760
                     /gene="aroA"
                     /locus_tag="LZB08_003034"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBM7316516.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGKTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3738..4817
                     /gene="aroC"
                     /locus_tag="LZB08_003035"
     CDS             3738..4817
                     /gene="aroC"
                     /locus_tag="LZB08_003035"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBM7316517.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4820..6016
                     /gene="pheA"
                     /locus_tag="LZB08_003036"
     CDS             4820..6016
                     /gene="pheA"
                     /locus_tag="LZB08_003036"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBM7316518.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6063..6872
                     /gene="aroE"
                     /locus_tag="LZB08_003037"
     CDS             6063..6872
                     /gene="aroE"
                     /locus_tag="LZB08_003037"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBM7316519.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6884..7417
                     /locus_tag="LZB08_003038"
     CDS             6884..7417
                     /locus_tag="LZB08_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBM7316520.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7438..8277
                     /locus_tag="LZB08_003039"
     CDS             7438..8277
                     /locus_tag="LZB08_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBM7316521.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3528 a          848 c         1535 g         2671 t
ORIGIN      
        1 ttttctttgt tataattatt aaacaagaag aataataaat tataaaaatg gaggataata
       61 ctatgaaagg aatattaaaa gaactaacag caaaacttta tagctttttt tactttatgt
      121 tctggggctt ttattttagc gctggctatt tcttttacaa aaatatatgt acttcctttc
      181 tggtgaggcc ttattttagt gattttgagc ataagtaaat tttataaaaa tgattatttt
      241 atatatttat ataagattta gtgcttgata gattatttta gagaactcat tagtgagttc
      301 tcttttttat tttaaatata aaattataat attgggaggt agtaaaaatg atagtagtat
      361 taaaaatggg agcagacaaa aatgaagtta aaaaattaat tgaggctatt gggagagaag
      421 gggtagaagt taatcccata gatggaacag aattaactgt tttaggttta gttggagata
      481 ctagtaaaat agatgccaaa agaatagaag caaataaggt agttgaaaaa gttatgcatg
      541 tggtagagcc ttttaaaaag gccaatagaa aatttcatcc agaaccttca atcataaatg
      601 ttaatggaat ggaaattggt tcaaagaaaa ttgctatgat agctggacct tgttctgttg
      661 aaacagaaga ccaaatagta tctatagcta aagatgtgaa aaaatcaggt gcaggatttt
      721 tgagaggtgg agcattcaaa ccaagaacat caccatacgc tttccaagga ttgaaatatg
      781 atggattgga tttattaaaa aaagcaaaag aaaaaacagg gcttcctata gttacagaaa
      841 taatgtcaac acaagatata gatatatttg aagaaaatgt agatgtaatt caagttggag
      901 ctagaaacat gcaaaacttt gatttattaa aagaattagg taaaacaaat aaaacaatac
      961 ttttaaaaag aggattatct gctacaattg aagagtggct aatgtcagca gagtatataa
     1021 tggctggagg aaatgaaaat gtagttcttt gtgaaagagg tataagaact tttgaaacat
     1081 atactagaaa tactttggat ttaagtgcaa ttttagctgt gaagaaactt agtcatttac
     1141 cagttatagt tgacccaagt catgcagcag gtaaatcatg gatggttgac tctttatcta
     1201 aggcagcaat tgcagttgga gcagatggtc taataataga agttcataat gacccagcac
     1261 atgcactttg tgatggaaaa caatcgatta agccaaatga atatgatgaa ttgataagtg
     1321 aattaaagac tatcgcatca gcagttggta gagaaatata atctataaaa ggatggttaa
     1381 atgtggaaga aaaactatta gtaaatttaa aagaaaatag ctacaatata tttattaaaa
     1441 aaaatatact aaagaatata ggagaagaaa taaaaaaaat atattttgga aataagattt
     1501 ttattgtaac tgatgaaaat atagcaaaat tatacttgga taaagtaaaa aataatttaa
     1561 gtgaatctgg atttgaaatt aaatctttaa tattaaaatc tggtgaagaa acaaaatcat
     1621 ttaatactct tcctaaaata tacgatagtt tacttgactt taagttaact agaaaagatt
     1681 tgattattac tttaggagga ggggtaatag gtgatttagg tggatttgca gcagcaactt
     1741 ttttaagagg tataagtttt attcaaattc caacatcttt attagcacaa gttgatagtt
     1801 ctgtaggtgg aaaagttgga gttgaccttg agagaggcaa aaatttagtg ggaagctttt
     1861 accaaccaaa ggctgtgttt atcgatccaa atgtattatt atcacttcca gaaaaattct
     1921 atagagatgg aatggcagaa gttataaaat atggatgtat aaaagacaaa acttttttca
     1981 ataaactttt aagtttaaaa aatagagaag aagtaatgca acatatagaa tacattatac
     2041 ataaatgttg ctttattaaa aaaactgtag ttgaagctga tgaaaaagac tttggagata
     2101 gaatgcttct aaactttggc catacattag gtcatgctat tgagaaatac tacaatttta
     2161 cgggttatac ccatggagaa gctgttgcca taggaatgta taagataact ttagaaagtg
     2221 aaaagatagg aattacaaaa aaaggtacat cagaacttat aaaatcaatt ttaatgaatt
     2281 ataatttgcc ttatgagatt gaattaagag ataataaatc tatagtagat gctatatctc
     2341 ttgataaaaa aaatctaggt gatacattaa aaataatact tttaaaagat attggagaga
     2401 gtattatata taatactact tctaaatttt ttcaggaggt aagataatgg gaagtctaaa
     2461 aatatatcca agtaaattat ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca
     2521 tagggcagtt atttgttcat ctctaagcaa tggaaaaagt agaatttcta atatagattt
     2581 ttctgatgat ataatagcta ctataagagc aatgacatca ttaggagcta ttatagaaaa
     2641 gaaagaagat attttagaaa tttcgggaat tttttctaaa gaaggtattt taaatagaga
     2701 gaatcagttg aatcaaccta aattgacaat agattgtaat gaatcaggct ctacattgag
     2761 atttttagta cctatatctt tagcatttga tggtgtaaaa aggtttatag gtagaggaaa
     2821 tcttggaaaa agaccacttg atacttatta tgagatattt gacagacaga atataaaata
     2881 tagttacaaa gaaaatcagt tagatttgat tatatcaggt aagcttaaac cagatgaatt
     2941 tagagtaaaa ggaaatataa gttctcagtt tatcacaggt ctactgttta tattgcctac
     3001 gctagaaagt gattcaaaga taataattac cactgagcta gaatctaaag gatatttaga
     3061 tttaactctt tcaacgataa aagattttgg ggtagaaatt ataaacaata actataaaga
     3121 atttattata aaaggaaatc aaacgtataa agcgagagat tataaagtag aaggagatta
     3181 ttctcaagga gcattttatt tatcagcaga tgctatagga gaagatatta gcatattaga
     3241 ccttaaagaa gattctcttc aaggggatag tgaagtagtt gaaattttat ctaggatggg
     3301 aatggaaata ttaagagaag gcaacaaaat aaaaggaata acaaatggtt taaatggtac
     3361 tttgatagat gcatctcaat gcccagatat aatacctgta ttatctgtag tagcatcctt
     3421 aagtataggg aagactacaa taattaatgc tggtaggctt agaattaaag aatgtgatag
     3481 acttcatgca ataaatgttg agttaagtaa acttggagca aatatagagg agaaagagga
     3541 tagtttgata atcgaaggag taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa
     3601 agaccataga attgctatga cacttgcaat tgcttcttgt agatgtgata aaccaataat
     3661 attaaaagat tttgagtgtg tatcaaaatc ttatccacat ttttttaaag attttaagat
     3721 gttaggaggt cgaattgatg agtggaatat ggggaaataa tttaaaagta tcaatttttg
     3781 gagaatctca tggaaatgct ataggtataa atatagatgg tcttccttcg gggattgaac
     3841 ttgaccttga taatattgat aaagaaatga agagaagagc acctggaaaa aattctatat
     3901 ctacttcaag aaatgaaagt gatataccag aaattctaag tggatatttt aatggaagaa
     3961 ctacaggaac gccattatgt gcaatcataa gaaatagtga tactcgttca aaagattatg
     4021 gagagcttaa aaatttaatg agaccaggac atgcagattt cacaggtaat gtgagatatt
     4081 ctggatttaa tgactataga gggggaggtc atttttcagg taggataaca gctccattag
     4141 tattttgtgg ggctatatgt aaacaaattt tatcacaaaa aggaatagag ataggagcac
     4201 acataaaaaa aattaaaaat atagaagata tgagttttga ttatgtaaat atttcaaaac
     4261 aacagttatc aaatttgcaa actttggaac tacctttact tgatttatca aaggaagaag
     4321 ccatgaaaaa tacgattata gatgcaaaaa atcaaggaga ttcagttggt ggtataattg
     4381 agtgtgcagt agtaggtatt aatgtagggc ttggaaatcc attttttgac tctgttgaat
     4441 ctactttatc acatttattg ttttctgttc ctgccgtaaa aggtgtagag tttggtttag
     4501 gttttgaact tgcagatatg tatggctctc aaagtaatga tgaaatgtat tatgaaggaa
     4561 atcaagttaa gagcaaaaca aataacaatg gaggaattat aggagggatt actactggaa
     4621 tgccaataat tttcaaagtg gcaattaagc caactccatc aattagcaga caacaaaaca
     4681 cagttaatat aaaagataaa aaggacgata ttttatatat taaaggcaga catgaccctt
     4741 gtatagttca aagggctata cctgtaatag aagcagtaac agccattgga atttttgatt
     4801 tgatgaaagg acgataataa tggaggattt aacgagatat agaaatgaga ttgacaatat
     4861 agataaagag cttattcaat tatttgaaaa gagaatgaat acagttcttg aaattgccag
     4921 acataaaatg aaaaataaca ctactatact tcaaaaagat agagaagaaa aagtactaag
     4981 tagagccgta gataatttag ataataaagc atattcacaa gaaacggtac agttttttaa
     5041 ttctataatg gaaattagta gaggattaca aaaaagattg atagataatg atggtgagca
     5101 gaaaatcgag tttagtttag attcatcaaa taagaaattt gatttgtcaa atgtaaataa
     5161 atataagtct ttaaaagatg aattaaataa aaaaaatatc ctagtagggt ttccagggaa
     5221 atcaggttca tttacagaag aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa
     5281 acaatttaaa gaatttgaag atgttttcat agcacttaaa aataaagaaa taaactatgg
     5341 aataatcccc atagaaaatt catctacagg agctatctct gaaacttatg accttttgag
     5401 aaagtacggt ttttatatag ttggagaaga atgtataaaa atagaccaaa atttaatagg
     5461 gataaaagac acaaagcttg aagatataaa agaaatatat tctcatccac aaggtatagg
     5521 acaatgtagt gaatttttaa aacaaaatag tacatggaaa ttgataccat ttaataacac
     5581 agcaacaagt gcagaacttg taagaaaact tcaagataaa acaaaagcag caattgcaag
     5641 caaaaaagct gcaagtattt atggattaga aataataagt ccttgtataa atgatatagc
     5701 aaacaactat acaagatttg tagtaatcag taatcaaata cacattgaag aagaatcaaa
     5761 caaaatgagt gtagtatttt ctgttgaaca tgaagcagga aaactttata aagtacttgg
     5821 atactttgct aaaaataata taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc
     5881 ctcatggaga tatttctttt atatagattt tgagtgttct atatacaatg ctaaagtgta
     5941 cgatttatta gaattgatag aacataatac agcatatttt aaatttatgg gagtatataa
     6001 aaataaaact gaatagataa gataaatttt tatttaattt ataattatta ggaggtgtaa
     6061 gtatgaattt tttcggtcta gtaggtgaaa aactctctca cagtgtttca cctcaaatcc
     6121 acaagagagt attcgagata ttgaatattg aaagtgctta taaaaacttt gaaatttcaa
     6181 aagaggatat atctaagcta gacggtgcta taaaattatt aggaatacaa ggagtaaatg
     6241 ttactgtacc atataaggag cgtattatga aatatcttga ttttatatca ccagaggcaa
     6301 aacgtatagg agcagttaat acaattttac ttagagaaaa tatgttatat ggttacaata
     6361 cagattactt tggtcttgat agtatgttta aaatggctaa tattgatgtt caaggaaaag
     6421 tagcagttat attaggaact ggaggtgctt ctaaagctgc tcttacatat tttattgatt
     6481 ctggtataga aaagctttat gtttcaacta gaaaaaagga tgataaaaaa ttattaaata
     6541 gtaaagctat attaatagat tatgaagaac ttaaacatat aaaaggagat ataatattaa
     6601 atgctacacc agtaggtatg taccctaatg taggaatttc accagtgtca aaatctatta
     6661 tacaaaattt tgatatatta atagatttaa tttacaatcc tggagaaaca gagtttttaa
     6721 gaattggtaa tagtatgggt aaaaaaactt gtgatggatt atatatgctg gtagggcaag
     6781 caataaaatc tcaggaaatt tggcaagata ctaaaataga taatagtatt ttagatgtta
     6841 tatataatga actaaaatta gaatttttgt aagggagatt gttatgataa ataaaacaaa
     6901 agaaaaattg atattaatag gtatgccagg tagtggaaaa actacaattg gtaaactttt
     6961 ggccaaagaa tataattgta gtttctgtga tatggatgat tatataattc aaatatctca
     7021 aaaaagtata gcagagttgt tttcagaagg agaagatatc tttaggaact atgagactca
     7081 agcatgtaga gaactttcta taagtgataa aacagtgata tctacaggtg gaggtgtaat
     7141 aaaaaaggat gttaatatgg aaatactaaa agaaacaggt ataataattt ttattgatag
     7201 acctatacaa aagattttgg aagatataaa tattaattca agaccattat taaaaaacgg
     7261 aaaagataga ttgtataatc tttacaatga gagaataaat ctttataaaa aattttcaga
     7321 tatagagatt ttaaatgata aatcattaaa taatgctgta tataatatta caaatgcagt
     7381 cagtgaaaat tttaaatttg actttaaaga aaaataatct agggggaaat aaaaataatg
     7441 aatattgtta ttgtaggact aggagttata ggaggctctt ttgctaaagc attaaaaaaa
     7501 gctggatatg aaaatgtatt tggagtagat gtagatttag aaactttaaa aaaagcagaa
     7561 aaagcaaaaa tcataaaaaa aggatgtact acaggtaaag agttatttaa aaaagctgat
     7621 ttaattatat tatctatata tcctagatta gttgtagatt ttctaaataa taataaaaac
     7681 ttttttaaaa aagggactat aattactgat actacaggaa taaaagaaac acttatcaat
     7741 gatgtgttgc aaattattcc agatgatata gattttatat ttggacatcc aatggcaggt
     7801 agggaaaaaa agggtattga ttttgcaagt gaacaagttt ttaatggcgc aaattatata
     7861 attactccaa ctggcagaaa caacataaaa aatttagagt tagttgaaaa tttaatttta
     7921 gagattggat ttaaaagagt aaaaaagtta acttcacaaa aacatgatga gattatagct
     7981 tttacttctc agttacctca tgttatggca gttgctctta taaatagtga tgaagaaggt
     8041 agagatacgg gtaaatttat tggagatagt tatagagatt taactagaat tgcaaatatg
     8101 aatgaagact tatggagtga acttttttta ggaaatagag ataatttact aaaagttata
     8161 gaaaactttg aatcagaagt aaatttagta aaagaagcta tatttaacaa tgataaaaat
     8221 aaattaatag agtattttaa aaaatcatca ataagaagag aagctttaga aaaataaata
     8281 ttaatattta agctatatat agatgggtat aaatataagc gatagtgtat ttaattgtat
     8341 taataaatat ggattcaata taattaaata tgctattgat atatttgtac ccttaactat
     8401 tgttatcttt tagatgataa tgatatttat ttaaatactc tttattaaca atttataaaa
     8461 cttaattgta aaaaatactt attaatagta aaataaagtg aatcaattat attataattg
     8521 ctaaagagag tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca
     8581 at
//
LOCUS       DAFKMX010000168         8385 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFKMX010000168 DAFKMX010000000
VERSION     DAFKMX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.170"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            374..1534
                     /locus_tag="LZB08_003040"
     CDS             374..1534
                     /locus_tag="LZB08_003040"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBM7316522.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1536..2666
                     /gene="glgD"
                     /locus_tag="LZB08_003041"
     CDS             1536..2666
                     /gene="glgD"
                     /locus_tag="LZB08_003041"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBM7316523.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSEKKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2695..4137
                     /gene="glgA"
                     /locus_tag="LZB08_003042"
     CDS             2695..4137
                     /gene="glgA"
                     /locus_tag="LZB08_003042"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBM7316524.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4151..6571
                     /locus_tag="LZB08_003043"
     CDS             4151..6571
                     /locus_tag="LZB08_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBM7316525.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRM"
     gene            6576..>8385
                     /locus_tag="LZB08_003044"
     CDS             6576..>8385
                     /locus_tag="LZB08_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBM7316526.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     KINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIEN"
BASE COUNT         3373 a          815 c         1550 g         2647 t
ORIGIN      
        1 ctttggataa ttatttaaca aatattagat tatattttgc tattacgaat ttaacaaatt
       61 aaaaaataag catgtttata tgacacaaag gatttataaa aaatgttttt atatttataa
      121 aataaaattt gtattatttt ttaaccgata attgaatatt aataaatact atagtgctta
      181 tggaaaataa tgtatacgtc ggtaagtcca ttatggggta tacattatat caattgtata
      241 gttgatatta ttttattttt attaaggtat tatttaagca acatttatac acgacattta
      301 ttgactgata aagtttataa aagtaaaaat gcaattataa atattaatta ctagatgagg
      361 ggagtgtcat taaatgaaaa aagagatgtt agctatgatt ttggcaggag ggcaaggttc
      421 aagattaggt gtttttacaa aaagaattgc caaaccagct gtatcatttg gaggtaagta
      481 tagaattata gattttgtgt taagcaattg ttcaaactct ggtattgata ctgtaggtgt
      541 tttaactcaa tatagaccgt tgatattaaa ttcacatata ggcatgggaa gtcattggga
      601 tttggacaga ataaatggtg gtgtctatgt tttacaacca tttatgaatg aaaaagaagg
      661 aaattggtat aatggtacag cacatgcgat atatcaaaat atggactttg tagataccta
      721 taatccagaa tatgtattga tattatctgg tgaccatata tacaagatgg attatagcaa
      781 aatgttaaaa tttcacaagg aaaaaggctc taaagctaca atagcagtga ttgaagttcc
      841 ttgggatgaa gcttcaagat ttgggataat gaatacaaat gaagattcaa gtatctatga
      901 atttgaagaa aaaccaagtg aacctaagag caatttggca tctatgggag tatacatatt
      961 tgattggaaa atgttgagaa attattttaa ggaagcagaa aaaaatccag aaataaatta
     1021 tgatgatttt ggaaaaaatt taatacctaa aatgttagaa gataatgttg gaatgtatgc
     1081 ttatccattt aaaggatact ggagagatgt aggaactata caaagtctat gggatgctaa
     1141 tatggatata ataaaatctc cagaaacact tgatttagca gaccctaagt ggaaaatata
     1201 tacaaatact atggcaatgc ctccacaata tataggtaaa aatgcaaatg ttcatagatc
     1261 aatgatagct gatggttgta gaattttagg tgaagtaggt aactctgtat tatcacatgg
     1321 agttgttgtt ggtaaaggaa gtaaagtaat tgattcagta ataatgccaa atgttgtaat
     1381 aggtgaaaat gtaactatag aaaaagctat gataggtgag tgtgcaacta taaatgataa
     1441 tgttcagata aagaatgtaa ataatgaaat aaatgtagtt tctgagtatg aaaatatcga
     1501 acctagatgc gtattaatag aaggaggtct ttaagatgag aaatgaatgt ttagggataa
     1561 taaatttaaa caaaaaagga gacccagcaa taaacaaact taactatgga agaccaatag
     1621 catctacacc aatagctgga agatatagaa taatagactt tgctttatct aatatgataa
     1681 attcaggaat cactaaagtt ggaatattcg caaaagaaaa atacagatca ttaacagacc
     1741 atataggtag tggtaaagat tgggatttaa gtagaaaaaa aggtggccta tcaattttta
     1801 gtccagaaaa cactaagtat agaaatactt actcacatag agaaggtgat atatatacta
     1861 tcttagctaa tctagattat atagaaaaga gtgaagaaga atatatttta atagctccaa
     1921 gctatatgct atgcaattta aattattctc aagcattgga ataccataaa aaatctcata
     1981 atgatataac tataatatat aaaaatgtaa ataatgcaaa taaagatttt gctggtaact
     2041 taactctaaa tttagatagt aacaataggg taataaatgt aggaaataat ttaggtaagt
     2101 tccctagagc aaacatatgt atggagacat atattatgaa gcgtgaagat tttgttgaat
     2161 gtatatataa cattgtaaat aaaggcaatt attgttattt agaagaattt attattgaag
     2221 aagcagaaaa catgaaaata ggagcctatg aatatacagg atatctaaaa tgtgtaaatt
     2281 ctgttgaatc atattttgaa atgagcaaag atttattaaa tttagaagtt gcagatgaat
     2341 tattatattc tgaaaaaaag atatttacaa aagaaaagaa tgaatcacct acaatttata
     2401 cagatagtgc taaagtagaa aatagcttta tagcatctgg ttgtttaata gaaggtactg
     2461 taaaagatag tatcatattt agaaaggtaa atgttgaaaa aggaacagtt atagagaact
     2521 caatagtaat gcaaaattgt gtaataaagt caaatgcaaa actatataat gcaatattag
     2581 ataaaaatac gagtgtatca aatgggaaag aattaagagg agatgaaaaa tatcctcttg
     2641 tagtggaaaa gaatacaaat atataaataa attttttaga agggggaatg actaatgaag
     2701 gttttttatg taacagcaga gtgttggccc tttgcaaaaa ctggaggatt aggagatgtt
     2761 tcttatgcat tgcctaaaga attaaaaaaa gaaggtgtag atgtaagggt aataatgccc
     2821 aaatattcaa ctataccaag ttatttaaaa gatcaactaa aagaaatagc tgtgtttagt
     2881 gtacgtgttg gatggagaaa tcaatactgt ggtcttttag aaatggaatt agatggtgta
     2941 aaattttact ttatagataa tgaattttat tttagaagag aagatgaaag aaagagtatc
     3001 tatggatatg gagatgatgc agaaagatat actttcttta cagatgcagt tttagaagct
     3061 ataagtagaa ttgattttta tccagatgta atacatataa atgattggca tacagggatg
     3121 ttaccattaa ttctaaaaga aagatatgca acccttgagg ggtataaaaa tataaaaact
     3181 atgtatacta tacataatct ccaatatcaa ggtgtatttg ataagcatgt tttatatgat
     3241 atattggatt taccacaaaa atactttgat aatggagata tagagtatta tggctcaata
     3301 aattttatga aagctggtat aaattttgca gataaaatta taactgttag tcctacttat
     3361 gcaaatgaaa tacaaacatc tttttatggc gaacaattag atggattact tagaaaagag
     3421 tcaggaaaat taaaaggtat attaaatggt atagattatg atttaaatga ccctgcaaaa
     3481 gataaagata tatttgttca ttatgatgta gactcaattg acaaaaaagt agaaaataaa
     3541 ttgagattac aagatatatt gggactaaaa aaagattcat ctattccgtt gataggtata
     3601 gtttcgagat tagtttctca aaaaggattt gatttaatag catacatgat gccagaactt
     3661 atgagagaag atttacagat tgtcgtttta ggaactggag aacatcaata tcaatcaatg
     3721 ttcaactatt acgattctaa tttttcagat aaagtatcag ctagaattac ttttaatgca
     3781 tcattggctc aacagatata tgctgcaagt gatatgttct taatgccatc attatttgag
     3841 ccttgtggta taggtcaaat gttagctatg agatatggtt ctttaccaat agttagagag
     3901 actggagggc ttagagatac agtaacacct tataataaat ttactggaga aggcaatgga
     3961 tttagtttta aaaattacaa tgctcatgag atgtttttct gtttgaaaaa tgcaatcaaa
     4021 gtatttaagg acaaagaaaa atggattaaa ttagttgaaa atgcaatgaa aacagataat
     4081 agttggaaaa aatcagctaa ggagtatata gaaacatata gagacatatg tgattaaagg
     4141 agtggttttt ttggtaacaa taagcaaaaa gaaattcaag caaaaatttg aagtaaaaat
     4201 gtatagctta tatgcacaat ctatcaagga agctactgat gaacaattgt taaatgtatt
     4261 atgtagtcta ttgaaagatg agatagcaaa aaaatgggtt gctacaaagt tagataagaa
     4321 aaaagaagtt tattatttta gtttagaatt tttaataggg agacagttaa aatctaattt
     4381 attaaattta aatattgaag aagaagttag agaaggttta tctgaattag gaatcaactt
     4441 ggatgattta attgaagctg aagtagaccc agccttagga aatggtggac ttggtagatt
     4501 agcagcttgc tttttagatt ctatggcatc tttgaacata agtggtcaag gatatggaat
     4561 aaggtataaa tatggtctgt ttgaacaaaa atttgtaaat ggttatcagg tagaagtgcc
     4621 tgataactgg cttacagaag gacgatatgc atgggaaact gtaagaccaa atgaagcaac
     4681 aatggtgaag tttggtggtg aggtagaatt aatcaaggaa ggtagtcatt taaaagtaat
     4741 tcataaaaac taccttcctg taatggcaat gccttatgat ataccaataa tcggatacca
     4801 aaaccaatgt ataaatactt taagattgtt taagagtgaa ataccaaaga gagattttgg
     4861 tgaacttacc tcaaatgctc taaattattc tggtagctat gaagaggctt taaaacataa
     4921 gtattatact gaggaaatat cacaggtatt atatcctgat gattcaaatt atgcaggtaa
     4981 gttgttgagg ttgaaacaag aatacttttt tgtaagtgca gggatacaag atataataag
     5041 aaaatataaa aagaacaaat taaatattaa taatttattc gataaggtag caattcatat
     5101 aaatgataca catcctacct tatgcatacc agaacttatg agaattttgc ttgatgaaga
     5161 aaatttatct tgggatgaag catgggaaat aactaaaaaa acagtgtcct atactaatca
     5221 tactataatg tcagaagcaa tggaaaaatg gcctgtaagt atgatgaaag agctattacc
     5281 aagaatttat atgataatag aagaaataaa tagaagatat gtagaggaat taaataataa
     5341 aggatatgac caagatagaa taaaaagaat gagtataata gactgtgaca atatcaatat
     5401 ggcaaactta tgtattgtca caagtcatag tgtaaatgga gttgctaagc ttcatactca
     5461 tattcttgaa acagaggtat taaaagattt ctatcaagat gaaccaaata aatttaataa
     5521 taagactaat ggaattgcac ataggcgatg gcttataagt tcaaatcctc aacttagcaa
     5581 tttaattaca gatttaattg gtgattcatg gaagaaagat acattacaat taaaaaatat
     5641 tgaaaaattt aaaaatgatt cctctgtttt acaaagactt gatgatataa aatacaataa
     5701 taaagcaaat ctagcaaagt ttataaaaga taaatacgat ttaaatgtag acccaagttc
     5761 gatatttgat gttcaagtta agagattgca tgcgtataaa agacagttac tgaatatatt
     5821 taatgtactt catatgtatc atgaattact tgataatcct aatttgaatc ttgacccaag
     5881 aacttttata tttggcgcaa aagcagcacc aggatattat ttggctaaat gtataattaa
     5941 atttataaat tctgttgcaa gtactataaa taatgatgtt agagtaaagg acaagttaaa
     6001 agtagtattt ttagagaatt atggagtatc attagcagaa ataataatac cagcagcaaa
     6061 tgttagtgag caaatttcaa ctacaactaa ggaagcatct ggaacaagta atatgaagtt
     6121 tatgatgaat ggtgctataa ctttggctac acttgacggt gcaaatgtag agatatgtga
     6181 acaggttggt aaagaaaata tgtttttatt tggacttagt gcagaacaag tactaaatta
     6241 taataaatat ggtggatatt cttcacttga cctatatcat tctaatatgg atataaaaag
     6301 ggtagtagat gatttaataa atggatttat tcctaatctt ggcgaagaag gaagaagtat
     6361 ttataattca ctcactactt acaatgatga gtattttgta ttgagagatt ttgaaaatta
     6421 tggtcaagct caagcagata taaacaggtt atatagagat aaagaaaaat ggaataaaat
     6481 gtctttagtt aatatagcta actctggatt tttttcttct gatagaacaa tttctgagta
     6541 tgcaaaagac atttggttta aaaggatgtg attaaatgca aagtgtaata gaatataact
     6601 cttgggataa aaattttaag gctccgtttg gagctttaaa atttgatgag gaattgacca
     6661 tttgtgttaa agtaaatgag ggatacaata tcaagtctat ttccctaaaa ataaatagag
     6721 aagaagaaat gagaacaata actttaaatg aagaattaga taatgataaa cttggaaagt
     6781 gcttttgtgg taagattgaa aagtttgatg gtacaggtgt ttatttttat tactttaaag
     6841 ttgatgttga aatggatggt cagataaaga ctttatttta tggaaaaaac agagataatg
     6901 gatattcttg tgaatataat tatagtgata ttaataaata tcaaatcact gtttacaaag
     6961 attttaaagt accaacatgg tacaaagaag gaatacttta tcatatattt gtagatagat
     7021 tcaataatgg aaatcgtagt ggtaaggttg ataatcctaa aaaaaatagt ttcatatatg
     7081 gaaattggga agatacacct atgtacataa aggatagtca aggggatgtt ataagatggg
     7141 atttccatgg tggaaattta agagggataa tcaataaact tggatattta aaaaaattag
     7201 gtgtaagtat actatatcta agccctatat ttgaagcctc tagtaatcat aagtatgata
     7261 caggtgacta taaaaaaatt gacccaatgt ttggggatga agatacattt aaagaactta
     7321 ttgataaagc aaaagaaaaa ggtatatcaa tagtattaga tggagttttt agtcatactg
     7381 gagcagatag taaatatttt aatatgtatg gaaactataa tagtttaggg gcatatcaat
     7441 ctaaagaatc tccatattat tcatggtata tgtttgaaga atttcctcaa aaatataaaa
     7501 gttggtggga tgttaagaca ttgccaaata taaatgaatt agaacatagc tatatggatt
     7561 atataatata tgacaatgat agcgttataa ataaatgggt aaatatgggt attaagggct
     7621 ggagactaga tgttgcagac gaattaccaa ctaagtttat aagagagtta aaaaaggaat
     7681 taaaaaaggc agatgatgac tcaattctta taggtgaagt atgggaagat gcatcaaata
     7741 agattagcta tggtcagaga agaagttatt tattaggtga agaattagat tctgttatgg
     7801 gttatccttt tagaaataac atgttttctt tcttaaaagg agaaattaat tcatatgaac
     7861 tttgtaatag atatatgcag attaaagaaa attatccaaa agaatcattt aggtctaatt
     7921 taaatttaat tggaacacat gatgttacaa gagctaaaac ggaattaaat gatgatgtag
     7981 atttagtgaa gcttgctgta gctatacaga tgacatttga aggagtacca tatatatatt
     8041 atggagatga agcaggtctt tgtggaggtg ttgacccaga taatagaaga acttatccat
     8101 ggaaaaatga agacgaagat atgcttaatt tttatagaga tgttataaaa attagaaata
     8161 agaataaact tttaagttct ggaaatacag aatttatata tactaataat gattctgtat
     8221 tttcttttat tagagttaat gaaaataatg atagaatgtt aattttagta aatagaagtg
     8281 aaaatgtgga aagtatatca ttatgtatag agagtagttt tattgaggaa atacctataa
     8341 agtatagtct taaaaatgca aatagtacta tacaaattga aaata
//
LOCUS       DAFKMX010000169         8182 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFKMX010000169 DAFKMX010000000
VERSION     DAFKMX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.171"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            4..906
                     /locus_tag="LZB08_003045"
     CDS             4..906
                     /locus_tag="LZB08_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBM7316527.1"
                     /translation="MVYILMIIRIILFRDVPIYAIFKGTFRNVNLIPFYTIYQFIVDS
                     NLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIVIILVSLAFELTQYIFALG
                     SSDIDDVILNSLGGIIGINIYINMNKLFPSDINRFKAIIGTSLVLGVIGLGVISKNYH
                     NLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVK
                     RPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVW
                     IDKENQPKDKHGIMASKLLIGLYE"
     gene            1021..1806
                     /locus_tag="LZB08_003046"
     CDS             1021..1806
                     /locus_tag="LZB08_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316528.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKYKKQQEQLEHFKKIINEKR"
     gene            2098..3417
                     /locus_tag="LZB08_003047"
     CDS             2098..3417
                     /locus_tag="LZB08_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBM7316529.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3492..3920)
                     /locus_tag="LZB08_003048"
     CDS             complement(3492..3920)
                     /locus_tag="LZB08_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316530.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(4011..5387)
                     /gene="hydG"
                     /locus_tag="LZB08_003049"
     CDS             complement(4011..5387)
                     /gene="hydG"
                     /locus_tag="LZB08_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBM7316531.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5388..6473)
                     /gene="hydE"
                     /locus_tag="LZB08_003050"
     CDS             complement(5388..6473)
                     /gene="hydE"
                     /locus_tag="LZB08_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBM7316532.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6509..7717)
                     /gene="hydF"
                     /locus_tag="LZB08_003051"
     CDS             complement(6509..7717)
                     /gene="hydF"
                     /locus_tag="LZB08_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBM7316533.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7732..7983)
                     /locus_tag="LZB08_003052"
     CDS             complement(7732..7983)
                     /locus_tag="LZB08_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBM7316534.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2909 a         1124 c         1195 g         2954 t
ORIGIN      
        1 tttttggtat atattttaat gattataaga ataattttat ttagagatgt accaatttac
       61 gctattttca agggaacatt taggaatgta aatttaattc cattttatac aatatatcaa
      121 tttatagtag atagtaatct tgactttatg aaagctactg taaatattat agaaaatata
      181 ggtatattta ttcctatggg aattttttta cctatagtat gtaaaaatct aaataaaaaa
      241 actataatta tagtaattat acttgttagc ctagcatttg aattaacaca atatattttt
      301 gctcttggaa gtagtgatat agatgatgtt attttaaatt cattaggtgg cataattgga
      361 attaatattt acataaacat gaataaatta tttcccagtg atattaacag atttaaggct
      421 ataataggta ctagtttagt tttaggagtt atagggttag gagttatttc taaaaattat
      481 cataacttac taacttttaa atttagacct gatagaaaaa tatcaaaaat attaattgaa
      541 gaaaataggg aaataattaa agatataaat aaagatacag tagatatagt tgggacattt
      601 gagagcttta aaaaaggaat aataactatt aaagcaggtt ctaataacaa agtaaagaga
      661 ccagagaatt taattgattc tgatggaaat ataagaattt atttaaatga aaatacaaaa
      721 ttagtatctt atataattac caaagaatca aaaatggata tagttaaata tgaggaattt
      781 gatgtgaaga atttagattt attaaggaga tatgatacta ttaatgtttg gattgataaa
      841 gaaaatcagc caaaggataa acatggtatt atggctagta aattattaat tggattgtat
      901 gaataaaata ttaaacaaga ataaaagaat aacaaatata aaaaacgaaa aaactatttc
      961 tataattatt tttacgttgt gcataaaata agaacattag tgaaggagta gagataaatt
     1021 atggagataa taaaatatat cttgtacttt tttatatatt catttttagg atggacagta
     1081 gaaagtatag gatgttctat agcaagcaaa agaataatca acagaggttt tttaaatggt
     1141 ccaatatgcc ctgtttatgg ttttggagcg gtaattgtga tatcactatt gggaagattt
     1201 aataatgtag taatagtatt tctattagga atgattttaa ctacgatact agaatatttt
     1261 acaggattta ttttagaaac cttatttcat gctaaatggt gggattattc tgacagaaaa
     1321 ttcaatataa agggtagagt atgtttaaaa aatgcaatat actttggtgt aatgtctgtt
     1381 ttaataataa gatttataca tccatttata aaatattttg taagtataat tccatataga
     1441 atattaattt caatggctat aattacaaca ctttggacta tacttgattt gattgttact
     1501 attataacac ttaaaaaatt agatataaag ttaaacttac tagacgatat aattacagac
     1561 cttaatgata taaatgtgaa attggataaa tttgatagag gagaaataca agctttattt
     1621 aaaacaataa ataacgacga attagaagtt agagaaaaaa taaacaaaat aaatagtaag
     1681 ttggaccgta tagagtctaa tattatttta caaaaaagag ttataaaagc ttttccacat
     1741 ataaaatata aaaaacaaca agagcaacta gaacatttta aaaaaattat taatgagaaa
     1801 aggtaattat acaatttaat gaagacgaat agaataatgg tatttataag atgattttca
     1861 gaggactcta taaaaataga atagagtcct ttattttttg aaaaataatg tttatttgat
     1921 aaaaataaaa tatattgttt ctgaataaca aatattttga aaattataaa aacatatatt
     1981 gacaatttgt gtcaaaaata ctaacattaa gtatataaag aattttatat gagaatatct
     2041 ttataaatcg caataatttt aaaacataaa tttatttaaa gaggggatga tttgccaatg
     2101 agcgagataa ctagtcaaat gataagtaca gaagaaaaaa aatatgttgc taaaactcaa
     2161 aagatacctt attatccagt agcatttaaa tcaggtgatg gtgcaatgct atatgattat
     2221 gaaggtaatg aatatgtaga ttttttagca agtgcaggct ctgcaaatgt aggtcatggt
     2281 aataaggaaa tctcacaagc agttaaagaa caaatggatg atataacaca atatacactt
     2341 gcatatttcc atagtgatcc tcctgtaaaa ttagctgaga agcttgtgga aatagcccct
     2401 ggagataatg ataaaaaagt actatacagt gcaactggtt ctgcttgtat agatgctgct
     2461 ataaaattag caagagggta tactggaaga acaaagataa tatctatgtg tgaatcttat
     2521 catggaagta cttatggagc tatatctata tctgctttaa gtacaaatat gagaagaaaa
     2581 atgggtccat tgttaccaga agtttatcac ttccattatc ctgataaaaa tagaacagct
     2641 aaagaatgtt tagatgagat agaatatgct tttgcacact atctaccagc agaagaggta
     2701 gctgcaatat ttatagagcc aattgcagga gatgcaggaa taatagtgcc tccagtagag
     2761 tgggtacaag gtttaagtaa aatatgtaaa gaaaatggaa tattacttgt aagtgatgaa
     2821 atacaacaag gtatgggaag aacaggtaag tggtttggta tagaaaattt tggtgttgaa
     2881 gcagacctta tagttcttgg aaaatctgtt ggaggtggat tacctctagg agcagttgtt
     2941 ggaagaacag aaataatgca aagtcttgat gcaccagcac atttatttac tttagctgga
     3001 aacacaacag tatgtgtggc tgctttaaaa tctatagaga taattgaaaa agaaaactta
     3061 cttcaaaaga gtatagaaat gggagattat attaaagcag gatttgaaaa actaaaagaa
     3121 aaatacgata taattggaga aataagaggc ataggattat ctataggagt tgatatagtt
     3181 aaaggaaaag gttctaatga aaaacatcca gatgctacag ctaagatatg ttatagatgt
     3241 atacaaactg gattaataat gatattctta ggacaatcta ctttaagagt gcaaccacct
     3301 ctagtaataa ctaaggagca agtagataaa gcaatgaaca tcatagattc tgcaatagat
     3361 gattatttaa atggtagaat tggtgatgaa gtatatgaag taactcaagg ttggtaaaat
     3421 aaatattaaa tgaaaatagg agttatctca attttagaaa attatttctg aaatgagata
     3481 actcttttaa gttacccaca aataagaaaa ttgtttgatt cattaaagac tatatttcgg
     3541 atgtcgtttt tgtgagtatc agagacagta ttacgagaat aattaccttt attcgtacta
     3601 tctgttaatt tagttataat taagatagct aaaagaataa gaattactgt gtataaagca
     3661 ataacatatg gttttctttt atatttgtat ttgttattga aaaaagaatg gccataatta
     3721 tttttattta ctttagatgt atttgtattc tttttcttgt tacctttctt tttatcatcc
     3781 ttttttctat ttttagggta aggaatgact ttgtgtgtat tatcaagttt gatattttta
     3841 tttttttgaa tattgttttc caggctagcc tttttttctc caaattgaat agttttattg
     3901 gtattgttgt tatctttcaa tgttaatctc cctcccttaa actttccctt tagataatat
     3961 tactactaac ctatggaata tacaataaat aatatataag taataaaatt ttataggtat
     4021 aaatctcttt ctccacgctc taaggcagtc atattgttag atactatgtt tcttatatct
     4081 tctctttcta tcttacctat ttcttctgcg ataagttttt tacccataat tttaagttct
     4141 tcatctccat aatcgattaa aaattcattt aatgtagtaa gagcatttgg tgtacataca
     4201 ttatggattt gtcgactttt agctaagctc atgaatctat cgccagttct acctttacga
     4261 taacaagctg tacaataact aggtatatat ccatcagtaa tcagttcttt aagcacctca
     4321 acagggcttc tatggtctcc aacttcaaat tgttctgtat tgtcaccatc ttcatatgct
     4381 ttatatcctc caacacctgt tagagaacct gcactaattt gtgatacacc atattttagt
     4441 aactcatttc tcatttcagc agtttctcta gtggacatta tgattccagt aaaaggaaca
     4501 gctaatctag ttatagctac tatttttttg aacatatcgt cagaaactaa gtgaggaaaa
     4561 tcttctaagc tcataccttc agcttttttc aatctaggga aagatatagt atggaaacct
     4621 acaccaaatt tttcctctaa gtgttcgttg tgcatcataa gtcctagaac ttcaaattta
     4681 gggtcagata aaccaaataa aactcctgca ccaacatcat caacaccagc ttccatagct
     4741 ctatcaaaag ctgttaaatg gtaataatag tcatttttta tagattgacc atgcattttt
     4801 ataaatgttg gtttgtgata agtttcttgg aataatatat aagttcctat tcctttttct
     4861 tttagtaatt tgtactcatc tacacttgta gctgctatgt taacgtttac ccttcttata
     4921 tttccgtttt cattatatgt tgaataaatt gcatctaagc aatctaagat gtaatttata
     4981 tcacaatttt tagggtcttc accagcttct agagcaagtc ttttatgacc cattttttct
     5041 aatattttaa cctcttcctt tatctcttcc atggtaagtt tttttctttt aaatttgttg
     5101 catttactaa aaccgcaata aacacattca tttacacagt agtttgatac ataaagaggg
     5161 gcaaataaaa ccactctatt accatatatt tcatttttta tttgcccagc tatactaaat
     5221 aatctatcta gttgtttttt atcttctact tcaagaagtg tagctatatc tttataacta
     5281 agtttttccc ttctatcagc cttatcaagt accttttcaa tatcatcaga agttgaattt
     5341 tttgcatctt ctaataaaga attaataagt tcatggttta tgaacattta caaacgcctc
     5401 cattcaatat tatcaccgcg agaatagtca acttcaagtc ctaatgactt gatgttttct
     5461 tctaaacttt ggtgatattc acaagcttct tgaccagtaa aggctttgtt atcgtaaaga
     5521 gaatattgtt ttctaaattc ttgaggagaa aggtttggca taattacatt gcaaccagct
     5581 aaaagacctg catttctacc actagggttt atagatgcca aggctgttgt agcgggtaat
     5641 aaaacttttg gaagtagaag tcttgtaatt gaaagcataa gtaatgtctt ctctaaatct
     5701 ccatgtttat aatccttaaa aattgtatca tggtgtggaa tgaaaggtcc tattccaacc
     5761 atatgaggat ttaactcttt taggtataat aaatcacgaa caaggtcttc gttgttctga
     5821 aatggtgatt caaccataaa tccagcacct acctgataac caatttcttt aaggtttttt
     5881 aggcattctt ttctagttct aagctcaatg tttggcggat gtaatttttt ataatgactg
     5941 tctgtagcag tttcatgtct taaaagatat ctatctgcac cacataaaaa gtatttttta
     6001 tacgaatcat aactcttttc tcctagtgat agagttatgg cacaatcctc atatttattt
     6061 tttatacttg aaacaatttc acatatttta tcatcagtaa aatatgcatc ttcaccacct
     6121 tgcaaaacga atgttctgta tcctatttta taacctatat cacaacattc aagtatttca
     6181 tcaagtgaaa gcctgtatct tatgatattt ttattagaag attgtattcc acaatagtag
     6241 caattatttt tacaatagtt tgtaagctct ataagtcctc tcaagtagac tctatttcca
     6301 taatgtttat ttctcatgtc gctagcttgt ttataaagat agtttcttaa attttgttcg
     6361 aagttatatt cctcattgtc gatattttct attaaatata gtagttcatc aaaacttaat
     6421 gaattaatgc tgtaaagttt atctataaag tatttaatct tatttttagt catattcaaa
     6481 tatataatta ttaagtatag ttaaattttt aataattgaa aagttcaagt gatctttcca
     6541 aaataccttt tacatatgct attaagatac catagtttac aataggtaca ttgaaagatt
     6601 ttgccttttc tattctagat aacattccag ctctattcat catacaagct ccacagtgaa
     6661 ctacaagggc atatttattt atatcttcag tgaaggatac tccagaacta aattcaaaag
     6721 taatgttttt gccagttttc tttctaatca tattaggaat ttttactgtt ccaatatcat
     6781 cagtttgtct atgatgggta catccttcgg ccatcaagat tttatcacca tcttttaaat
     6841 tttctaaagc ataagcgcca ttaatcaatt ctttaaggtc tcccttttgt ctagcaaaca
     6901 atatagaaaa agatgtaaga ggaatatctt ttggtgtatc tttatctact tgtggaaata
     6961 cttgagagtc agtaataaca agttttggtt ttttgcctaa attagataga gtttctttta
     7021 aactatcttc ttttgtaact atagaaattg caccactatc caaaatatct cttataactt
     7081 gctgttgagg aagtataagt ctaccttttg gagctgcttt atctattgga acaactaaaa
     7141 caactaaatc atttggttca attaaatctg ataccaattt gaactcagtg ctatccttag
     7201 gcaaaacttt tattatttca ttttttaaat tttcaattcc aattttatct gttgatgaga
     7261 cagaaacaac tggacattta actttatttt tagttaagtt taaaatttct gattgattct
     7321 taatagtatc aattttgttg agaattagaa tatgagggat attcttatca ttaaattttt
     7381 caattagtga caaatcttct tgtgatatac caatttggca atccactact agtaaagcta
     7441 tatctgtctt ttctaaaaca tctagtgatt tacctattct aagttcacca agttcaccaa
     7501 catcatcaag accagcagta tctataagaa cacaaggtcc tataggaagg atttccattg
     7561 gtctaaatac tgggtctgta gtagttccag caatatctga gactattgca gcactttgat
     7621 ttgttatagc atttattatg ctagatttac ctgcatttct tttaccaaag agacctatat
     7681 gtactcttac cgattgtgga gtagaattta gactcatttg tactcaccac cttaaacttc
     7741 tttttttgat atactagttt ttacagatac atgaggtatg ttgcctaatt taccagtcaa
     7801 actattaata gtatccattt cgccaacaac tgctatacat acgattgaga caccttcttc
     7861 ttcgcaagga atgcccattc ttcctttaat aataccttta aaacctgata caacctcatt
     7921 gaatttaaat tgacattctt ttggctcttc caaaatagca cttataactg caaccttttt
     7981 cataaatatc ctcctaactc agaaaatatg tcttaaacaa atccaaaatc aaaaaacttt
     8041 tctttgtaaa gaaaagtttc tacaaaagaa aagttattaa ttacacaagt ataatgtgct
     8101 atattaaact atttccttac agattagata actcttttct ggtagggtta gcttgtaaaa
     8161 atttaattta acaacaagta ac
//
LOCUS       DAFKMX010000170         8062 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFKMX010000170 DAFKMX010000000
VERSION     DAFKMX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.172"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1059
                     /locus_tag="LZB08_003053"
     CDS             <1..1059
                     /locus_tag="LZB08_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBM7316535.1"
                     /translation="MDNDHFILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVM
                     SSGIYKITKKDDIKKIDLLIDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDN
                     MNKALEKGEFIVYYQPKYSLDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFI
                     VNIDMFVFEEVCKTLNKWINKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPE
                     FIELELTESVVFDNLNILIDIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLD
                     RGFLIETTDTKRGKIIISKIVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLF
                     AKPMVLDEFEKHLNFKFD"
     gene            1206..1952
                     /locus_tag="LZB08_003054"
     CDS             1206..1952
                     /locus_tag="LZB08_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316536.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGSSIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            1952..3832
                     /locus_tag="LZB08_003055"
     CDS             1952..3832
                     /locus_tag="LZB08_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316537.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            3951..5342
                     /locus_tag="LZB08_003056"
     CDS             3951..5342
                     /locus_tag="LZB08_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7316538.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5449..6489
                     /locus_tag="LZB08_003057"
     CDS             5449..6489
                     /locus_tag="LZB08_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBM7316539.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            6978..7631
                     /locus_tag="LZB08_003058"
     CDS             6978..7631
                     /locus_tag="LZB08_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBM7316540.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKAFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            7707..7916
                     /locus_tag="LZB08_003059"
     CDS             7707..7916
                     /locus_tag="LZB08_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316541.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         3222 a          757 c         1358 g         2725 t
ORIGIN      
        1 atggataatg accattttat attgttgatt gcttgtaaca ctgaagatga acttttaaat
       61 agattatcta aaataaataa agaaatttgt aacttaaata ctactaactc atctaagtat
      121 aaaattgtta tgtcaagtgg aatatataag attactaaaa aggatgatat taaaaagatt
      181 gacttgttaa ttgatagagc aaatattgca gcaaaaagta aaaaggaaaa atatgaacat
      241 tcatattcat tttttaatga agatacaaga aatcgattgt acaaggaaaa acgattggaa
      301 gataatatga ataaagcttt agaaaaaggt gaatttatag tatattatca acctaagtat
      361 agtttagatg atgtaaatga aattgaagga gcagaagcct tgataagatg gaatagtcca
      421 gaatttgggt ttatttcacc tatagatttt gtacctttat ttgaaaagaa tggttttatt
      481 gtaaatattg atatgttcgt gtttgaagaa gtatgcaaaa cattaaacaa gtggataaat
      541 aaagggtata ctcctgtacc aatctctgta aatatgtcta gagttcattt atatagggat
      601 aattttatag aaaatataac tgatttgata agtaaataca atatatctcc tgagtttatt
      661 gaacttgaac taacagaaag tgttgtgttt gataatttaa acatattgat tgatataatg
      721 aaaaagatta aagagatagg attccttata tcaatggatg attttggttc gggatattca
      781 tctttaaatc tgttaaaaga cctttctttt gatattttaa aattagatag aggattttta
      841 atcgaaacaa cagatacaaa acgtggaaaa attattattt ctaagattgt ggaaatggca
      901 aaagcgattg atattaaagt aatctgtgag ggcgtagaaa cttatgaaca agtagagttc
      961 ttgaaagaga taggttgtga taaggttcaa ggatacttat ttgctaaacc tatggtatta
     1021 gatgaatttg aaaagcactt aaactttaaa tttgattaat cattaataat tattagacta
     1081 tgagataccc taacaactta cttacaaata tgtaagttaa ttgttagggt atttttatgg
     1141 ttaaagtagt atattcacga tattatggta ttgtagtaaa aataaaaaat tatggagaat
     1201 ataaaatgaa catattgagt attagaaata tatcaaaaac atattgtgga aatattccat
     1261 ttaaagcatt agataaggtt agtctaaata tagaaaaggg agaatttgtg tctgttatgg
     1321 gaccatctgg aagtggtaaa tctactcttt taaatataat atctacagtt gatagacaga
     1381 gtgaaggtga ggttgtatta gatggatatg atgtaagtaa attgaaagga gaaaaacttg
     1441 ctgaatttag aaggaaacaa cttggttttg tatttcagga ttttaatttg attgacactt
     1501 taactgttgg cgaaaatatt atgttgccac ttacactaga ggggtcaagt atcaaagata
     1561 tgaatataca aactaaaagt atatctaaat ttttaggaat agataaaatt ttagatagaa
     1621 aaacctatga aatttcagga ggtcaagctc aaaggtgtgc tattgctaga gctattataa
     1681 ataaacctgc catacttctt gcagatgagc caactggaaa tcttgattca aaatctactg
     1741 atgatgtttt gaaattattt actaggataa acaaagaaca aaaggtaaca acacttatgg
     1801 taactcatga agcatatagt gcaagttatt cagatagagt aatttttata aaagatggtt
     1861 gtatatatac agaaataaaa aaatctgaaa gcagtaattc tttctattct gatatactag
     1921 ctgttttgtc tcaaatagga ggtgtaagat agtggatttt agaaagtttg cttataataa
     1981 tatagttaga aatttcaggg catatttagc atatttttta agttctgttt tttctataat
     2041 gatatttttt gtttttgcag tgtctatgtt ccatccaatt ataacaaaat ctggaatgca
     2101 gagtggttct actgcattta tggctcttat gacttcagaa ttaataatat tcggttttag
     2161 tctacttttt atattgtact ctttaggaaa tttcttaaaa tatagattga aagattttgg
     2221 agtattgatg ataattggaa tgagtaacag acaactgaaa aaattgatat taattgaaaa
     2281 tttgataata ggatttttag cagtgatgtt aggaatatta cttggtttat caatttcaaa
     2341 attgttttta ttgtatttaa gcaaactgtt ttatatgaat ttaactgaat tttattttcc
     2401 agtaaaagca atgctattga ctattgtatc atttatgatt ttgtttttaa taacaggacc
     2461 aatgagctta aaattattga gtaaaaagag tattttagag cttttagtag gtacaaaaaa
     2521 gcctaaaaaa gagataaaat cttcaaagct atttggaata attggtgtaa tatgtcttat
     2581 attaggatat gctatgatac tatttggcaa taagttaaaa ataaatggag gtatgtatac
     2641 aacagcacta ttaattactt tggggatatt tttatttttt tctcaattta ctattttgat
     2701 attaaagtat ttaaaaaata aagaaaaatt ttataaaaac aaagtaaata tgttacttat
     2761 aagtaatttg atatataaat taaaggataa tacacgattg ttatttttaa taacaatact
     2821 tttatcggct actttagtag catttacaac aacttcaaca cttgtaagtt ctcaaggaga
     2881 agcttctaaa aataatttcc caatggttta ctcatatttt tcagagcata ataatgaaaa
     2941 agaatatgaa gaactacaac aaattagaga agcaatcaaa ggatatgatt ataaggagtt
     3001 aagttttcct gtacttaaat ataataggga agtcttacta agtgtaaaga attataacaa
     3061 attatcaaag gaattgggat tagataaaat tagttcaaag ttagaaaaat atgaagggat
     3121 tataattcct agtagcaatt cgcaagaaca tataaattct ttaaaacaat taaaaaatta
     3181 tagtataaaa aatttaaata tagaagtaaa aaaatcaaaa aataaaataa tttttcctac
     3241 aggactgttt agtaaagttg tagttataag tgatgactta tacaatgaag taaaaggtga
     3301 attttcaaaa attaattttc atggatttga ttataaaaac tggcaaagca gtgcgaagat
     3361 aactgaaaat ttaaaagaaa aacattttaa tttggataga gaatataaca ggtcatattt
     3421 tttaacatta ccagatatgt atttagtaga gttacaacaa agtaaaattc ttcaatttat
     3481 gggtgttttt ataggagtga tattatttat agcagttttt agttttttat acttcagatt
     3541 atatacagac gagatagaag ataatgtaaa gtacaaaaaa ctatcaaaaa taggtctatc
     3601 ttttaaaaat atgaataaga ttgttagtat agagataggt acattgtttt ttataccgta
     3661 ttttatagca attataaata gcataatttc tttactgttt ttaaatcaag tattaaataa
     3721 tagttttaag ttagaaaatt tgggtatatt actactgata tcatctatat attcacttta
     3781 tttttactta ttaaaaaaaa tttatactag aaaagtttgg ttttttgatt aaagtggtat
     3841 ataatagata tataaattaa tatttaaaaa gtagttaata ttaggggagg gcatttgcac
     3901 ctcccttgta atatgtggaa aattgaatat tagaagaagg aggagacttg atggaaaatc
     3961 aacaattatt aggaacagaa cgaattagta aattactact aaaatattct ataccagcta
     4021 taataggtat gttggtaaat agtttatata atgtagtaga tagaattttt attggaaata
     4081 taccaggggt tggaccactt gcaataacag gtcttggtgt aacaatgcca ataatgacaa
     4141 taatacttgc atttgggatg ttgataggta taggaactac tactaccata tcaataaaac
     4201 ttgggcaagg taaagtagaa gaggcaagaa aacttatagg aaatgcaatg acgttatctg
     4261 taataacagg aattatcata atgatacttg gaatattatt tgcaaataaa atacttacat
     4321 tgtttggagc aagtgaaaat actttaatat atgcaaaatc atatatcaat attatattat
     4381 taggtacagt agtaaatctt ctttcctttt ctttaaacca ctcaataaga gctgatggta
     4441 gtcctaaaat ttcagcagga ataatgatag taggatgttt aactaatata gttttagact
     4501 ggatattaat atttggattc aatttgggaa tacaaggagc tgcaattgct acagtaactt
     4561 ctcaggctct tacagctatt ttaactatag ggtattatat aagtggaaaa tcaaacttac
     4621 gattcagtaa atctaatcta aaattagata aaaagctaat aaaagcagtg tttgcaatag
     4681 gtatgtcgcc atttgcgatg caacttgcag caagtttagt tcaagtaata tcaaatattg
     4741 cacttaaaac tcatggtgga gatttagcta taggggctat ggctactatt tcttctattg
     4801 caatggtatt tttgatgcct atctttggaa taaatcaagg agcgcaacct attataggtt
     4861 tcaattatgg agctgaaaaa tatgataggg ttaaaaaagc atatttaggt tcattagtag
     4921 tagctactat aattttatgt atgggtatgg tagtagttat gcttttccca gaagctatta
     4981 taggtatatt taataaagac cctgaactta tgaatatatc tgttaatgga ttaagaatat
     5041 acttacttat gttgccaatt gtaggcttat cagttacagg aacaaacttt atacaatcta
     5101 taggaaaagc aaaaatggct atgttattga gccttttaag acaagtaatt ttattgatac
     5161 cagcagtatt aattcttcct acattcttag gtttacaggg tgtatggaca gctcaaccag
     5221 tgtctgattt tatagctaca gtaattacag gtatagttgt atttagagaa ttaaaaagat
     5281 atactcctaa aactgaaaaa ttaaatgaaa atgaaagact aaacgagata actactgagt
     5341 aaaaatataa attcagataa ttgagcaaga aattgcaaaa aatacgatta tatgataaaa
     5401 taaaaccgct gtcaaggata agagaaattc taattatgga gtgattttat ggaatttaca
     5461 aagaaaagca aattaaaaaa tatgtatgta aacatgaatg tagctagaat gttgtctaag
     5521 ataaatgagt ataagggtag acaactgctt tacaaaaaac aaccaaaaga aattttagaa
     5581 aacttagaaa aaaaatcttt agtagattgt tctgaatcta catacattgg aaatcaaaga
     5641 gatagtagta attttaatct agaaaagttg ataagtaatg aagttacacc tagaagtaga
     5701 gaggaattaa gtattgttga atacagagat gtggtaaaaa ctattaacag tgcttatgag
     5761 agtattccca tcagttcaca aacgattctt gagcttcatg gatacttata taaattttca
     5821 tctactagag gaggaagtta taagtcagat aatgatttta ttgaacacaa tttaaaacct
     5881 agtgaattta taagtattga ttcaagtgtc aataaagttg aaaaagctgt agaagaaata
     5941 tgtgaagctt ataatacatt gatagaggaa gatgaaattg atatattaat attaatttct
     6001 gcatttgtgt tagatttcat attgatacat ccattcaaag aaggtaatat aaaaatggcg
     6061 agagtactta tcctattgtt actaaataaa aatgggtatg aagttggaag atatataagt
     6121 ttaggaaaga tatttgatga tagttcatat gaatattata gtaatttaaa ttatttgaaa
     6181 gcctcaatag gtagtgaaaa agcagatatg aatgcatgga ttgagtattt tttagagacg
     6241 atattaactg catatgagaa gctggatgat agtttaaata tatctgataa gaaaagacaa
     6301 acaaaaacta gtagaataga gaaaataata aattctaccc ttggatattt tacaaaagaa
     6361 gatataagag atttatgtcc tgacattcca gaacctacta taaatagagt ttttaataat
     6421 ctaagaaaac aagataagat agaagttgtt gcaagaggaa gaagtgcaaa atggaagaaa
     6481 aaatattaaa aaatgtttag ataactaaaa tggtatagtg ttttacacta taccattttt
     6541 aatgtcatta gtatgagcaa taatttaaca acttaagcaa tatcaatttt atttattgat
     6601 tatttttgaa ctgaaaaatg gcgttatatg gtatatctca gtggtaatta attttggatg
     6661 agaagatata atattacaga atatattagg agtatatgta gaaaaaatat aataaaagtg
     6721 tgaggcttag taattaacaa attatgtggc ttcacacttt tttataaaat aaagatactt
     6781 agcagaataa tattttagtt gatatgttta taagatggta cttgtttggg taaaagtata
     6841 aatagtaaaa aagtagaagt aatgtgtata aaaacataag ttacattctg taataatagt
     6901 tattttataa ttcaacaaaa gaatagaatg ggtatatttg tgaaataata atataaaata
     6961 atcttaggag tgagcttatg aagaaaaaat tattatatat aaatgtaaat tcaaaacctg
     7021 aagacttatc ttcaagtaaa actgtagcaa gaaaatttat aaataaattt atggaaaaaa
     7081 ataaagcttt tgaggttgaa gagatagacc tttataaaga acatatacca agacttgagt
     7141 atcagtactt tgaaaaaaga aatagcatag ttagtgaaga aaatgctaaa aatttagacg
     7201 ataaggatag aaaagagctt acaaagatta gaaatctttg tgaccaattt gtgagtgcaa
     7261 gtgtatatgt aatagcagca cctatgtgga gtttatcttt tccagcacca cttaaagagt
     7321 atatagattg tataattcaa gatggaaaaa ctatatcttt tgaaggcaat gataaaccac
     7381 aaggaatttt aaatgatatt gatagaagta tggtatatat acagtcttct ggtggacaca
     7441 taccatgggt gttgaagcca gttatgaata aagggttaaa ttatgtggaa agtattatga
     7501 aatttatagg aattaaaaaa tttgatgaat tgttagtaga tggaactgga actagtgaag
     7561 aagaaagaca ggctgcaata gaaaaagcat ctgaaaaaat agatggtata atagatggga
     7621 tgaaatttta attataatat tttattaaaa atataaagga aaaacctctt ttaatgtaga
     7681 aacttatatt gaaggaggcg agaatcatgc gagaagaaaa aagtaatgaa aagtatgatt
     7741 gttattggtg taatcaagag aataatttct gtgtagaaat aaaagataat atagtcatga
     7801 tagatgatgg cactggtacg ttaaaacaag ccgttttcat agggtataaa caaatccaaa
     7861 ttaatttaaa ttgttcacat tgtcaaaact taaatagaat aaaattaaat ttgtagaatt
     7921 ttaaaatgga aattattgaa ggaattaaaa atagaaagtg ttaatattta gactgaaaat
     7981 tatattttat acaataaaag tttatttaaa taacattgta ttttaatatt tattttcagt
     8041 cttactttat taaaattatt tt
//
LOCUS       DAFKMX010000171         7915 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFKMX010000171 DAFKMX010000000
VERSION     DAFKMX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.173"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..625)
                     /locus_tag="LZB08_003060"
     CDS             complement(<1..625)
                     /locus_tag="LZB08_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBM7316542.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFN"
     gene            complement(664..1524)
                     /locus_tag="LZB08_003061"
     CDS             complement(664..1524)
                     /locus_tag="LZB08_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBM7316543.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            1793..2002
                     /locus_tag="LZB08_003062"
     CDS             1793..2002
                     /locus_tag="LZB08_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBM7316544.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2002..2268
                     /locus_tag="LZB08_003063"
     CDS             2002..2268
                     /locus_tag="LZB08_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBM7316545.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2297..2869
                     /locus_tag="LZB08_003064"
     CDS             2297..2869
                     /locus_tag="LZB08_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBM7316546.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3104..4294)
                     /locus_tag="LZB08_003065"
     CDS             complement(3104..4294)
                     /locus_tag="LZB08_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBM7316547.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4554..4985)
                     /gene="dut"
                     /locus_tag="LZB08_003066"
     CDS             complement(4554..4985)
                     /gene="dut"
                     /locus_tag="LZB08_003066"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBM7316548.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5098..7326
                     /locus_tag="LZB08_003067"
     CDS             5098..7326
                     /locus_tag="LZB08_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBM7316549.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVNVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7437..>7915)
                     /locus_tag="LZB08_003068"
     CDS             complement(7437..>7915)
                     /locus_tag="LZB08_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316550.1"
                     /translation="NNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAYFEE
                     CPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDPKDR
                     RILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKLG"
BASE COUNT         2768 a         1249 c         1031 g         2867 t
ORIGIN      
        1 cgttaaatgg tggtcttatt atgtcacgta ttattaaaag attttgtata tctacattat
       61 ttgtgattgc ctcatccaaa ataaataaat tatatgcatc atttaatggg tcatgggcat
      121 ctccactaaa ctctaagtta acaaattcaa gtaaattttt caatgatata tagttcatac
      181 atctcatacc acaatttata tatttttctt ttatatctac aaataaatct ctaatgttat
      241 ttacaaacct tggatggtta ttttttttag cacttctcat atctgtgtta ttgaaacatg
      301 tcaaatccaa attaccaaaa gtatatattc ctttaatatc agaaaatatg ccaagccatt
      361 ttttaaactt tctcatgacc tcttcataac ttggtgcact cttcaattct tcagaagata
      421 tatgtgttaa ttcttgtata tgaggataca actcctcatt acttactggt cttattaaag
      481 atttaaattt atcaatattt tttgtttttt catcatacat aactgctccg attgcaatta
      541 tatcagaatt aaatactgct ctttttgatt tagaatttgg catattcatt tcaaaatcta
      601 tatatactct tttcaatata aacacccttt ccaattgttt atagtgattc tttttaacta
      661 tttttattca aaattttcta agaaagaatg tttctcccca tctttttgcc aaccaagtac
      721 tgaatcaaga tttacatttt ctgctgcctt aaatatagat ttttctacat ttttaaaatt
      781 tgattctaag ctctttatta agtcttttat ctcatatctt ttattacctg tttttttagc
      841 agcaaccata catgcacaat ttaatggcca tataccacta ttttgaataa atcttataat
      901 atgttcttct cttatataat aaagtggtct tataattttt ataccttcaa aattagtaga
      961 attaagttta ggaagcattg ttttaaaatt tccagcacac aataagttaa gcattgttgt
     1021 ctctattaca tcatcataat gatgacctaa tgcaagtttg ttacacccta attcttctgc
     1081 ctttgaatat aatgctcctc tcctcattct tgcacacata tagcatggat aatcttttgc
     1141 tatttcatct gctatctcaa atattcttga atcaaataaa tgtattggta tatttagata
     1201 ctcacaatta tctataagta attgtcttat atttgcatga tatccagggt ccatagcaat
     1261 aaattcaaca tcaaaattaa cttgtccatg tttttttaac tcttgaaaca ttttagccat
     1321 aagaatactg tcttttccac cagaaatcgc tacagctatc ttatcacctt cttcaaccaa
     1381 tttataatct cttattgcct tcataaactt agaccataaa ttttttctat atttttttat
     1441 tatacttttt tctatatctc ttactggaag tctttcctca aaaggaacta taatttcgca
     1501 gccttttcct gatagttcac tcatctaatc acctcgtata tttatgttaa taatatttta
     1561 caaaaaagtc taccttcatc cacaaaatac tataattttt actttaattt tactaaataa
     1621 tttatagttt acaataacac ataaacttat aataacataa ataacctctt tcttttatta
     1681 tcttttttat ttttctcatt taatatttta ttattagtaa cgatttttaa atgattgaat
     1741 attatacact agatagagtt acctatctat ttacaacaag gaggttttca tcttggaaaa
     1801 taagactaga gatatcaaaa aaaattgtgg tccatctttg gctagaccat acattaataa
     1861 tcaaattttc acagaaatgt actgcctttc tgatgcatta aaatttggaa ctatatttcc
     1921 agaactaaac ttactagagt ctgaaatgta caacaaagaa ttatatgcta agccaaaaaa
     1981 atatagagga ggtagaagat aatgaaagac tcatctagag ctgaacttat gagaaaagtt
     2041 caggaaacat cttttgcatg tgttgatatg aacttatatt tagacaatca ccctgatgat
     2101 aaaaatgcta tcaacactta taattcatta tgtaatcaat ttgcgcaagc tagatatgct
     2161 tacgaaaata aatacggtcc tttaactaac tttgggtatg ctccaagtag atgcccttgg
     2221 caatgggctg accaaccttg gccttgggac agagaattta attattagac atcaagtatt
     2281 ggaggattga aaattcatgt ggatatatca gaaaactata caacacccag ttaatataaa
     2341 aacttgtgac cctagaatgg ctaaatttct tataactcaa tttggtgggc caaatggaga
     2401 acttgctgca tctttaagat atttaagcca aagatataca atgcctactg gtaatatgcg
     2461 tgcactttta acagatattg gtacagaaga actagctcac gttgagctta tatgtactat
     2521 ggtttatcag ttaacttctg atgcaagccc agaagagtta aaagctgcag gtcttggttc
     2581 aaactatgct caaaatggat atggaattta tccaacagat tcaaatggtg ttccatttga
     2641 tgtaagacct atagcagtta tgtcaaatcc cgtaaccgat ttacatgagg atatggcagc
     2701 tgaacaaaaa gcacttgcaa cttattatca acttataaac ctaacagatg acgttgatgt
     2761 tatagatgta ttaaaattct taggtcaaag agaaataatt cactatcaaa gatttggtga
     2821 agccttaatg gatgcttacg agttagaaga atctcaaaaa atgttctaaa atttttattt
     2881 gtataactat tattatttga taatttataa tttactgttg tgttaaaatt tataaaaaat
     2941 ataaatataa tttattttct ataaacagag aaaataaata aaaagcaggt atcaatcaaa
     3001 tagtaacatg tattatacta tacattacta tcaatattgt acctgctttc atattataag
     3061 caataaagaa aaatctattt attaggtttt ataataaaaa caattataaa actcttcttg
     3121 catttacata tctacttgaa tagtagctac tatttatatt agatattgtt actttttttg
     3181 atgaacctga agatgcatgt atcatatctc catttcctat gtatattcca acatgactta
     3241 cagaaccatt atttgaacct tgtgtatcaa agaatactaa atctccactt ctaaggtctc
     3301 ctctatttac atatgttcca tacttacttt ggtctctaga tactcttggt atactaactc
     3361 ctacagattt tttcataacg tactgtgtga atccagaaca gtcaaaacta tttggacctt
     3421 ctgctcccca tacatatggt tttccaagta gtgtctttgc aaaacttatt acactatcag
     3481 ctccttgact tggagaatta gtcgaataag atggaattga agtatcaaca gaaccttttt
     3541 cagcaaggta ttgacttgac acatatccag tttgattgcc ataacttact tttgtccatc
     3601 ctccactttc agaaatactt ccaacctcag taccataact taatgtagct attttagaat
     3661 gacttgtacc tggtcctgtt ctaacgttca atgactttgc tgttacaacc tttgtaccct
     3721 ttacaaggtc atttgaactg ttttcagcat tttcatttgt agagtcattt acactagttc
     3781 ctaaatattt acttgaaaca taacctactc taccattaga acttattttt gaccatccat
     3841 ttgactcaga tatgtatccg acttcagtcc catatcctag tgttgatatt ttagaactac
     3901 ttgtacttgg tcctgttcta aaatttaatc cttttgctgt tacaactttt gtcgacttaa
     3961 ctgaagtatc actactttca ttgctacttc caaggctatt tattgctaca taatttccat
     4021 gcacataacc tgtgtttcca ttatatttaa cttttaccca agaaccttct tttcctacgt
     4081 actcaacttt atcacctttg taaagtttcc ccattgattc atggtttgta cctggtcctt
     4141 ttctaaaatt taccgcattc cctgttattg ttctgctttc tatactcaca ctctctactt
     4201 cagactcatt agcatatgct aaattcccca tagtaggcat tattatcgca gttgccatta
     4261 tactggctgc tattctcttt tgattaaaat tcataacttt ccactcatct cctcttttct
     4321 actatcttta tttcacttca aaatataaaa gaaataattg acactattaa aatatcaatt
     4381 atttctttta tattaatatt attacaaatt tgtaactatt tgtctatagt aatatttgct
     4441 taccataaat taccatgtat aaattttaat ctttaatagc ctgtaattta gtaatatagc
     4501 catcttttac cttttttaaa aatggctata ttttttaact tttttgtaac tttttaaaat
     4561 ccagttgagc caaacccacc ttttcctctt tcggagtcac ttaattcttt tacttcttct
     4621 atgttaatat catagatagg ttttactatc atttgagcaa ttttcatatg cttttcaacc
     4681 ttaaaatcat ttttaccatg atttattaat ataattttaa gctctcctct gtatccttcg
     4741 tcaatagttc ctggtgtatt taatacagta acagaatgct ttaaggcaag cccacttcta
     4801 ggtcttacct gcgcttctgt catagtagga agctctatac atatcccagt tttgataagt
     4861 ttagtctctc caggaggtat tacaacctct tctatagaat acaaatccat accagcatca
     4921 cctttgtgag caaaattagg tattatagca tcatcattta atttttttac ttttaaattg
     4981 tacatcttat atctcctttc aactttctat aaatattata acttaaattt cgacctcttg
     5041 aagtttttta tttttttgtg gtaatataat tattagtgtt ttaaaggagg attttttatg
     5101 aaacttgtac ttgctgagaa accatctgtg gcaaaaacta tagcatcatt tttaggtgca
     5161 aagaccagac aagatggtta ctttgaagga aatgattata taattactta tgcagttgga
     5221 cacttagtat cattatatga tatgaaggat tatgataaag ataaatactc tggctcttgg
     5281 aaaatgaaca atttcccatt tgttcctgag gataagttta aatttaagat tgatagttct
     5341 aaaactaaac aattcaacac tgtaaaaaag cttttgaata gaaaagatgt agaatatgta
     5401 ataaatgcca ctgacaatga ccgagaagga gaacttatag catttttaat atttttactg
     5461 gcaaaaaata aaaaaccagt aaaaagaatc ttggtaaatg aatggacacc agaagatatt
     5521 acaagaggta taaaaaacct aaaagatgaa gatgaaatga gaaatcttca agctgctgga
     5581 tatactaggc ttattactga ctggttgatt ggaataaatt tcacttctgt tgctacattg
     5641 aaatatggaa atgggaaact attaaacata ggtagggtaa ttcttccaac agttaaatta
     5701 gtctatgata gagatatgga aatactaaac tttgttccta aaacttatta tgaaatagaa
     5761 gggcatttta aagctgaagc tggtgaatat aaaggtaaat atgttaaggg taaagaaagt
     5821 aaatttgata cacttgaaga tgcaaataaa attatagctt caattacatc tgaaactggt
     5881 aaaatactag ataaaaaagt tactatgtca aaggaatatg caccaaaact ttttagtttg
     5941 acatcacttc aaggatatat aacttctaag tattccaatt ttacatctga taaggtttta
     6001 aatgtctgtc aatctctcta tgagggtaat ggtaagggtg gatacataac ttaccctaga
     6061 actgattcca ttttcttaga agaaagtttg gcttcaaaag cttctcaaac tttagataaa
     6121 ttaaaggttg gtcttgaata tgaaaataaa attaaatttt ctaaaactaa aagagttttt
     6181 gattcttcta aagtagactc ccatagtgct ataattccaa cttatataat tccaaaaagt
     6241 ttatctaagg atgaacaatt agtttatgat gccataaaag atagatttgt agcaaacttt
     6301 atgcctcctg ctgaatatga gaatacagaa attaagactg aagtagataa ttgtactttc
     6361 ttaacaaaag gtaaggtgct taaatcaaaa ggataccttg aggtatataa taaggaagaa
     6421 aaaaacgatt tacttccact tgtaaataaa gatgatgttg taaatgtgtt agaaataaaa
     6481 ccattaacta agcaaactac tccacctaaa ccgtatactg aggacacact tttaaaggct
     6541 atgaaaaatt gtggtaaaaa tgtgcctgaa gaagatacaa cagtgctatc tggatattcg
     6601 attggaacat cagccacacg tgctgatgtg ttaaagaaga taagtcaagt gggctatgta
     6661 aagaaaaaag gtaaatctta ttctataaca gaccttggga aaaatttagt tgaaattttt
     6721 cctgttaaag acttatttga tgtagattat actggaaaac ttgaaaaaag tttaagcgat
     6781 attcaaaaag gaaagtatac aagaaaagaa tatcttacta atatcattag ttttatatat
     6841 caaaatgtca atattataaa acaagatgct tcaaaaaata taaacacaga agcatatact
     6901 ttcgattcaa aaactaaaaa atttactaaa acaaaagaaa aaaaacagaa aaaagatgta
     6961 actaatactg aaaaagaagt tgctatatca aaggattcta ataacaaaaa agaagttaaa
     7021 aatggtgatg aaaatcaatc tcttggcaaa tgccccattt gtcaaggtga tgtactagaa
     7081 tttgacaaag gctttgcttg taaaaatcat aaagaatgta aatttgttat ttggaaaaat
     7141 gataagtatt taagtcttta taaaaagaaa gttaacaaaa caatggtcaa aaatatttta
     7201 aaaaaaggtg aaactaaagt aaaatcttta actgctaaaa acggtaataa atttgatgct
     7261 acattaaaat atgataaaaa tccagatact ggctatttca attggaaaat agagtttgac
     7321 aactaaatat tcataagaaa agaaatagca gtagtataat aatctatatt atattactgc
     7381 tatttctttt cttatgaagt tttaactttt ttaatagtga cttttacaat cacactctaa
     7441 cctaatttct ttactatttc tgataacttt aatgtatgtt catctaataa agttagttcc
     7501 tcttcagaaa gattagataa tttttttact agagattctt tgagaatagc ttttactgca
     7561 tcaaataatt taaatgcttt atctgttaat tcaaccctaa gaatccttct atcttttggg
     7621 tcaggaaatt tattgactaa accatgttcc actaatctgt caataatcgg agtcatattt
     7681 gattttaaaa ttcctaaatt agaagctatt tgagaaattg atgatgaatt tgatgtcata
     7741 agataaagaa ttaccttagc atgtgatgga gccattggac attcttcaaa ataagctttc
     7801 attatatcag agttattttg aaactttttt aacaaatcgc tttctttaaa tatattattt
     7861 tcaagcacaa ataaaaaatc taaaaaagag tccgctattt taagtatatt gttgt
//
LOCUS       DAFKMX010000172         7805 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFKMX010000172 DAFKMX010000000
VERSION     DAFKMX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.174"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..230)
                     /locus_tag="LZB08_003069"
     CDS             complement(<1..230)
                     /locus_tag="LZB08_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316551.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVN"
     gene            complement(298..711)
                     /locus_tag="LZB08_003070"
     CDS             complement(298..711)
                     /locus_tag="LZB08_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316552.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(1527..2156)
                     /locus_tag="LZB08_003071"
     CDS             complement(1527..2156)
                     /locus_tag="LZB08_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBM7316553.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2235..3197)
                     /locus_tag="LZB08_003072"
     CDS             complement(2235..3197)
                     /locus_tag="LZB08_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBM7316554.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRSEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3959..7627)
                     /gene="cbpA"
                     /locus_tag="LZB08_003073"
     CDS             complement(3959..7627)
                     /gene="cbpA"
                     /locus_tag="LZB08_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBM7316555.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AGEDANAGNAEGDANAGNAEGDANAGNAGGDANAGNAGGDANAGNAEEDANAGNAEGD
                     ANAGNAEEDSNAGNAEGDANAGNAEEDMNALVVEEATAEDPTSVGDFKISGGEINVDF
                     KFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTLT
                     PDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVPE
                     GGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIIIN
                     GGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAGIGGGYYTTT
                     KNALSPSVVDSSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVTG
                     GTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMNK
                     ISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQGVLYLWFSDQFKGSEVSVN
                     LVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEEDV
                     EKSFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDTNLGSDDLTKQVD
                     AGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKPT
                     DTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTASI
                     EWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDVTNVDVDGDGIPDINI
                     DTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDGN
                     PDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDPDN
                     GGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDS
                     GNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKP
                     DIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKP
                     DINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNIQTGDQSNIMLDLALMF
                     ISLFFLIKNLTNKYLRRK"
BASE COUNT         2463 a         1471 c          820 g         3051 t
ORIGIN      
        1 ttattaacct ttgatagtat aaattcagat gattcatcaa ctaaatcatt atatttttta
       61 ttataaattg aataacatcc aaaaactatt aaccaactca accctggaag agaagcagtg
      121 cttggacctt taaaaatata cttagtggct aatacagcta taaaaaaata agttattaca
      181 agcaactcca ggataagctt tgatcttaca ccagtttctg atttagacat ataaaatcac
      241 tccttaaaaa ttatattctc tatactcttt aattttcgtt tttatcaata ttatcactta
      301 gttgcatatt ccctttctat caaaatgtat atacaaaatt aatttagata aagattgtat
      361 aaataaaata gctaagagaa ttacacctat atccaaattt gatatatcca aacctcttgt
      421 ataatttgct gaaaagaaaa cagctattag caaaatagaa tataacatta tttttgatgc
      481 aatatgatca acctttaata acataagttt tgacgcctcg tcaactaaat cattatattt
      541 tttattatac atgaagtcac atgcccaaaa agcgaataaa attataaaag taatacaaag
      601 atttccctct ttaaaaataa acttttttaa taatgagtct ataaaaaaat aggatatcat
      661 aagtaactct gaaacaagat ttgttttaac accaagctct gatttagaca tataaaatca
      721 ccccttaaaa gttatatatt tataacacct tatgttataa atatataact tttccaaata
      781 aattgcaata gaatattgaa aatattcaat aatatcttca taaatatatt ttataaaaga
      841 ttttataata aacattacaa tacagtacta aattagttta tttcatctat catctcaatc
      901 atatcattta ctaactatta ttctaatgag catataacat tgaaatttat aattatacaa
      961 cctaaatgcc tcccaatgta ccctcaaaaa catcatttcc tgtaactgaa gtataattgg
     1021 tatattttat cactgaaata aaaaaacact agtcaataaa gactagtatt ttcaagtatt
     1081 ttatcattct ttttttaatt attggagacg aatcatcaga ataatgccca taatttatct
     1141 atatgcaaat tctaaattta aaataaagtt tcagataaat ttactattct atgctatgat
     1201 acaacagaaa aaactgaaat ctatgagtgg taacttcata taatcctagt tcctacagta
     1261 atcaaaactg aggtttattc tatatgtaac taatgataaa ttttattata ggagttatta
     1321 gctctatttg tgttactata agtagtaatt atatatatga taatttttta acacttttct
     1381 attgctatat agaaagtggt ttagaattta aattcaccaa aaagaaatct atattagaaa
     1441 ccactactct attccagcag attgtaattt tttgttcttt atatcacaat gctataccat
     1501 attaaattga aaaataaatt ttaaatttat ttgttacaat tcattaattc aaaaaaatat
     1561 tttgagtcat gagttttgta aagatttagc catgaattta atgcttctga ttcaaaaaca
     1621 tctaaaagtt tcaatacttg aaacgtaaat tccaacggag caattcctga agccgtaact
     1681 aaattttcat cactaactac agattccgtt ttataatatt tttctccaga gtaatttggt
     1741 attaccattt taagaaactc taagtcatta cttgtatgat ttctaaaatc taatagccct
     1801 tttttagcta agcctaatgt agctccacat atggctgcaa ctatagaatc ttgtattaaa
     1861 gcttctttaa ctttttctaa tattatctca tgattcatag ataaccaagt atttccccct
     1921 ggcaaaatta ataaatcctt actttccata ttaaactcat caactgtaat ttcaggtaaa
     1981 acttttaacc cacccattgt tataatagga tttttatcaa ttcctacagt aattactttt
     2041 agtggcttta atcctttttt aaaatatctt cctgtattta attcagcagt taaatatgcc
     2101 acttcccaat ctgacatagt atcaaataca tacatatata cttttctatt tttcatcttt
     2161 tttactcctt atgtaattaa tttatatatc taattataaa ataacttaac tgacatattc
     2221 agtcagttaa gctattaaat ttgataataa tcaattaatt ctagtaaatc ttcaattatt
     2281 ttcttcctaa ggcttagtgg ttcaattact tgtattgtct ttctataagg taaaagtata
     2341 tttggtatgt acttgcatat tacttcctct tcaagcataa atattatttt attaggtgtt
     2401 ctttctttca agtagtgccc taaaaaccaa tgttggctta aactatctaa agaatctata
     2461 ctcccttcta caactaattg aattatattt tgttcatctt ttatttcagg tataattcct
     2521 tttataaaaa attcacttgc tgaaaattct gtcggtctgt taaatgtatt tgatgtttct
     2581 atgatttcac taatcctatc cactctgaaa ctacgtacct cacttctcat attacaaaat
     2641 gcaactacgt accaattatt attccagtga acaattccat atggatttat aattctatat
     2701 tttgattttg ctttacgacc tgcatgatag ttgatttcta cagatagctc ctttaatata
     2761 ctattctcca attttttaag tactgattct atagacactt tcccaattgg ttttattact
     2821 tcaagggcta ttaagtggtt tgttaaaata tcttcttgtt tttgatttga atacattttt
     2881 aactttaata tcgatttatc taatgcttcg tctaagaagt atccttcttt tttagcaaat
     2941 agtgctgcat gaagtagaga tttttgctct tctatatcaa aaaaaagagg tgctttagta
     3001 aaattattta aaaggctata cccaccatta tgacctgtat ctgaaattat aggtactcca
     3061 ctcgcagaaa gtacatcaat atatcggtac actgttctta tatttatttc taatttttca
     3121 gctatctgtt ttgctgtaat ttttttgtca gagtttaaca tccacagtat agacatcata
     3181 ttgtcaattt tagccatgta gtttcacctc tttctatttg ttatataaag ttattatata
     3241 actttttgat tgatttttta caaaaaaaca ttagtaaact gctaataatt tatactaaga
     3301 tttatttcaa atatttatag atagtctttt tctatagtta agttatccat taaattacat
     3361 agtagtatat ccattttagt tgatatattt tcagctacct cccttgaata ttattacaat
     3421 aagtctaaat cttctttctt ccataaatat attatgagga aagactatca tctttaatgg
     3481 tttaatagta taaaagatat taatttaaaa aaatctattt ttatatcaga tgggtattgt
     3541 tttttataaa actgaaaaaa tcacctaata gaatctatga ttttttaaca taacctccat
     3601 aaattagtac caattatcac ttcaaattca aatttttatc tgaagaatat acaatcatat
     3661 ttccatactc aacataatat tgagtgtttg gcttgtattt acaaattatt attctaacaa
     3721 aaacaactat acatagatta aaatcatctt ctaaacataa ctactaaaat tgaattaatg
     3781 atatcaattc cattaatctc ttttaataaa aaattctctt ttctatatga aaattatttc
     3841 aaaactttta agttaaatac aaaaatgact ataaatagat ttttatttac acaaatctat
     3901 ttatagtcac tatagtttat ccaataaaat aataaaataa atatgtacac aataaatgtt
     3961 atttacgtct taagtattta tttgttagat ttttaattaa aaaaaacaag ctaatgaaca
     4021 ttaatgctaa atctagcatt atattgcttt gatctcctgt ttgtatattt tgacctacat
     4081 tactatctaa actttcatct gtatctatat ttatatctgg ttttccattc ccatctgtat
     4141 ctatatttat atccggcttt ccatccccat ctgtatctat atttatatcc ggttttccat
     4201 ctccatctat atctatattt acatccggct ttccatctcc atctgtatct atatttatat
     4261 ctggttttcc atctccatct gtatctatat ttatatctgg ttttccatct ccatctgtgt
     4321 ctatatttat attcggtttt ccatccccat ctgtatctat atctggtttg ctatttcctc
     4381 cattatctgg gtctggttta ctatttccac cgttatctgg atccggtttg ctatttcctc
     4441 cattatctgg atctggttta ccattgccac cgttatctgg atctggtttg ctatttcctc
     4501 cgttatctgg atccggtttg ctatttcctc cattacctga atccggtttg ctatttcctc
     4561 cattacctga atctggtttg ctatttcctc cattacctga atctggtttg ctatttcctc
     4621 cattacctga atctggtttg ctatttcctc cattacctga atctggtttg ctatttcctc
     4681 cattatctgg atccggcttg ctatttcctc cattatctgg atccggcttg ctatttcctc
     4741 cattatctgg atctggctta tttagatttg gattacctgt atcataacct cctgagccag
     4801 tatctacatt tttatcaggc ttccaataat catctactcc atcattatct gtatctattg
     4861 ctggacgatt ataatctgta tcagggtttc catctccatt ggtatctata tttttatcag
     4921 gtttccaaat accatctcca tttccacctt ctgtactagg cttccattca cctgtattat
     4981 cagtatctat gttaatatct ggcttccaat caccactcgt atctatattg atatctggcc
     5041 ttccatcacc atcagtgtct atatttatat ccggtattcc atccccatct acatctacat
     5101 ttgtcacatc taactttaac ttcttttcat cctcactacc ttcaatgtaa tttatatcat
     5161 ctccaatata ttttacagta atagtttgtt cagtagtaaa aatatgatga ttgctatcta
     5221 aaggtctcca ttcaatagat gcagtactat aattatgact ctcactaccc ttattatgag
     5281 tttttaattc tactggatct ttatattcct ttccattaat atagaattgt acgtaacctc
     5341 ttggagaggc acaatctggt ccttctttct tatctggtga aatagtaaca tttaatgtaa
     5401 acgtatctgt tggtttaaaa acatcagttt tagctttaga ctcttctaca actaatgaag
     5461 tttctgtatc agcaggaata atttctgcct tataatatgc tctatgcccc caataagctt
     5521 cattaaaagg atatacattt gaaaactgtg ttgatgtaat gcttaattga tattttccag
     5581 catcaacttg ctttgtaagg tcatcacttc ctaaatttgt atctggttcc attgtttcct
     5641 catttaaacg ttgagaacta atagtcataa attttttatc tgtgagtttt tcacctttag
     5701 gagtaattat tggaataccc ccatctttta atatccaatc taaaaatgat ttttctacat
     5761 cttcttcaaa tggtaatcca tcatatcttt taataatcaa atatggtgga gtatcatcaa
     5821 tagtaaattc ttcatattgc tttaaaaatt gagaacctga acctatatct ttaacataaa
     5881 aatcatctgg ttttatattt cctccagtaa ttttatctat aacaactaaa ttaacagata
     5941 cttcagatcc cttaaattga tcagaaaacc ataagtaaag aaccccttga tcatctgtat
     6001 aactaggtgc tccataagga taaatttttc catcaattag cattttaaaa tcatcaacta
     6061 atgtagtact aactttatct tgtcctaaat actttaaaga tattttattc ataaaaactt
     6121 ttgttttctt ttctaaatca ccgtatgcaa ctgctccacc taaacttttg aatgttgttg
     6181 cttttaatga accacctgtt actataacat ctcccgttgt agcacttagg tcaaaaatcc
     6241 aatctcccac tcctgtcata taaggtaaaa tcgttcctcc agttacaagt atacgatatc
     6301 ctctatttgt tcctggatta acacatccat ccccaaatgc actaccatgt gcacctccat
     6361 atgaatagga aagtccacca ttaattgtaa tatttccact ataaccagaa cctgtgctag
     6421 aatctactac tgagggactt aatgcattct ttgttgttgt atagtatcct ccacctattc
     6481 cagctccatg gtatgcacct ttagcagtta tagtaccacc attgattgtg atatctccac
     6541 cactaccatt accgcctcca cctattccag ctccatatcc tgtattacga ctcgattctt
     6601 gacctatagc agttatagca ccaccattaa taataatatt tcctccactt tcagtatttc
     6661 caccgccaat acctgcagaa taataatttc caacaacttt taaacttcct ctattattac
     6721 tatctagtag tgtaaggcga tctccttttt ttaacttgtc tccattttct agagttacat
     6781 ctctaggaat ttttccaccc tcaggaacta taaactctcc ttcaatatct ctatttacaa
     6841 cagaatcatc aataattaga gtagtacctt ttcctgctct catacctgga taatatacat
     6901 cacctgactc tagcttgttt tcactaccat cttgtaatat aacatgtgca tatgcaccac
     6961 catctttatc tggtgttaaa gtaaatgggc tattagatgc agttttaata tttactccat
     7021 caaatattaa ttcagctttt acatctttag gtataacaat cctatgtgtt gttggagtat
     7081 ttggattatt attttgaatt gtcatttgtt ttccactaag aatagttaaa acgccacttg
     7141 caaatttaaa atcgacattt atttctccac cactaatttt aaagtctcct acagatgtag
     7201 gatcttctgc agtggcttct tccacaacta atgcattcat atcttcttct gcatttcctg
     7261 cattagcatc tccttctgca tttcctgcat tagaatcttc ttctgcattt cctgcattag
     7321 catccccttc tgcatttcct gcattagcat cttcttctgc atttcctgca ttagcatctc
     7381 ctcctgcatt tcctgcatta gcatcccctc ctgcatttcc tgcattagca tccccttctg
     7441 catttcctgc attagcatcc ccttctgcat ttcctgcatt agcatcctct cctgcatttc
     7501 ctgcattagt actttcttct atagtatctg cgaaagtatt tgacgcatta aatgatgcta
     7561 ctgatccaag tcccattgag atacttaaaa tcattgacag tgctttcttt ttacattttt
     7621 gttgcataaa ttccccctcc ccttaataat ttttaatata cactgtatac atccttatat
     7681 acactctaat aattcttaat atacactgta tacatcctta tatacactct aataattttt
     7741 aatatatact taaaacttat atttgtcaat gaatatacag tttttttcaa ccttatatat
     7801 tataa
//
LOCUS       DAFKMX010000173         7746 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFKMX010000173 DAFKMX010000000
VERSION     DAFKMX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.175"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            107..1705
                     /locus_tag="LZB08_003074"
     CDS             107..1705
                     /locus_tag="LZB08_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBM7316556.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KADKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLNN"
     gene            1900..2700
                     /locus_tag="LZB08_003075"
     CDS             1900..2700
                     /locus_tag="LZB08_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316557.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(3436..3585)
                     /locus_tag="LZB08_003076"
                     /pseudo
     CDS             complement(3436..3585)
                     /locus_tag="LZB08_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3612..4121)
                     /locus_tag="LZB08_003077"
     CDS             complement(3612..4121)
                     /locus_tag="LZB08_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316558.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEALETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4943..5380
                     /locus_tag="LZB08_003078"
     CDS             4943..5380
                     /locus_tag="LZB08_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBM7316559.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            5519..6307
                     /locus_tag="LZB08_003079"
     CDS             5519..6307
                     /locus_tag="LZB08_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7316560.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILNWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            6352..6810
                     /locus_tag="LZB08_003080"
     CDS             6352..6810
                     /locus_tag="LZB08_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316561.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(6911..7261)
                     /locus_tag="LZB08_003081"
     CDS             complement(6911..7261)
                     /locus_tag="LZB08_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316562.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(7263..7646)
                     /locus_tag="LZB08_003082"
     CDS             complement(7263..7646)
                     /locus_tag="LZB08_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBM7316563.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGLLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
BASE COUNT         2834 a          967 c         1210 g         2735 t
ORIGIN      
        1 attatataaa tttacctaat taataaattc attctaataa gtttacttaa aaggttgtag
       61 ataaatagtt tcaaatatat atcctaactt ataggaggtt ttaattttga aagctgcaat
      121 ttattcaaga aaatcaagat ttactggaaa aggtgattct atagaaaatc aaatacaatt
      181 atgtattgat tatgcaaagt ctattggtgt aaaagatttt ttgatatatg aagatgaggg
      241 atttagtggt agcaatatag atagaccaca atttaagaaa atgatgcagg atgccaaaga
      301 taagaaattt agttacctag tttgttatag attagatagg atatcgagaa atgtatctga
      361 cttctctact ttaatagaaa aattaaatga actaaacata tcatttatat ctatcaaaga
      421 acaatttgat acttcaactc caatgggaag agctatgatg tttatatcaa gtgtatttgc
      481 tcaattagaa agagaaacaa tagcagaacg tataaaagac aatatgtatg aattagcccg
      541 tagtggtaga tggcttggag gcaatacacc tcatggattc aacagtgaaa aaatcacatt
      601 cttagatgaa aatctaaaag aaagaagtat gtataagctt caagtaaatg atgaacaaat
      661 ggagattgta aagctaatat acaataaata tttagaactt aggtccttat ctaagttata
      721 taaatatgtc tataataatg gaataagggg tcctcgtggt ggtaaatttg accctagttc
      781 tttatctaga atacttagaa atccagcata tgtaaaagct aatgatgaaa ttttagatta
      841 ccttaaaaat agtggtatgg atgttgtagg tactccagat agaaaatgtg gaatccttac
      901 ctatgctaaa aatacatcta actcaatagc agcaattgca aaacaccatg gagtcataga
      961 acctgaaata tggcttgaag tacaaaatca attagataaa aatcgtgata aaactccaag
     1021 gatatcaact ggaaaaactg ctcttttatc tggtcttata aaatgtggta aatgtggttc
     1081 taatatgaga attacctata aaggcaaaaa agctgacaag gatttaaagt attattatat
     1141 ttgtggtact aaaaaaacac ttgggtctga tagttgcaac tgtaaaaatc ttaatggtcc
     1201 attagttgaa agtttagtta tagataaaat taagaattgt aaagaagagt ctattataag
     1261 tgcattcaat agtaataaca ttaagactga taatgttttt aatattttta aatctgagac
     1321 taatttactt aaaaatcaaa ttttagataa ggaaagactt attaaaaatt tagttataga
     1381 gcttgcaaaa aatactggta gtactgcttc tgattatata gtttcccaga ttgagagcct
     1441 aaataatgag attagtattt taaaggataa gattaatact ttggaatcta gtaacatcaa
     1501 tattagtagt gacagtatta atattgatat tattgttagt aatttgaaga gatttaatga
     1561 tgaagttgat aatgccagtt tagatgataa gagattgtta ttatctacta ttattaatag
     1621 tatcgtttgg gatagcagtt taggaaatct taatattgtt tatatgggtg ctgaagtgga
     1681 taattctgtt tctttaaaca attagttgca cttttttagc tatagcagaa gcaataagca
     1741 tatcagataa ggttgcaata ctatctaaaa gaccagctac cataaaaaat atttacgata
     1801 ttgaacttaa catggatgga acaaaaacgc ctctcaaaac acgtaaagtt cctgattttc
     1861 aaaagtattt cgacatatta tggaaggagc tagatacaca tgaggcttaa taaaagatct
     1921 gaaggatata taaactatgt taaaggtgta aaaagagaaa aaagaaatgt attattctac
     1981 cagctattaa ttttaattgg ttttattgta atctgggaac ttttagctga cttaaatgtt
     2041 ataaatacat ttctatttag caaaccaagt gatatttaca atttatttat tcaatatgca
     2101 tttagtggtc aattatttaa acatattgga atatctgtat atgaaactgt tcttggtctt
     2161 gttataggta ctgtattagg tattttggta gcaattgctc tttggtggtc agaaaaatta
     2221 tcaaaaatat tagacccatt tttggtagtt ttaaatgctc ttcctaagac tgctttagct
     2281 cctataatta tagtttgggt aggtgctgga atagaaggaa tcgtcgtaac agctgtaacg
     2341 atttctgttg ttgttactat actttcagct tataattatt ttatgaatat agatgaagaa
     2401 aaaattaaga tgttaaaaag ctttggagct actaaatctc aaatattatt taaactcata
     2461 ttacctgcaa acacaggaaa tttaataaat ttaactaaaa ttaatattgg tatggcatgg
     2521 gtaggtgtaa tagttggaga atttttagtt tcaagatatg gaataggcta cttgatagta
     2581 tatggttctc aagtatttaa attagattta gtaatgatgg gagtatttgt attggctatc
     2641 tgtgcttggg caatgtacgc tgtagttaat attatagaaa aaatatacaa ttccagataa
     2701 ataaaaattt tatttagaat atttgatgta tcagtgatta taataaaaaa tgatttcttt
     2761 acagtaatca tttttttaaa ttaaaataaa gcgtaagttt ttgattatct tcttataact
     2821 catcatattg ctagcacagc tctattttat gggctttgct ggctttttat tgcctccaat
     2881 tatatacata atgcttaaat gcagtcattt ctttattacc tatgcttaaa tttctacaaa
     2941 agaaaagaca gtcaactttt taatcaaaat taactgtgct ttatatttaa tatttaattt
     3001 ttatttttgt gctctgtttg ttagatagca gagacttaat tttgtatttt gaacaggatt
     3061 gaatcttaaa aatttcccaa ttatatcaat ttcaaagtcc attaagaata atcctataag
     3121 aactttgcta atttggtcta agaatattaa ggatataact ggaaaaacaa aagactttac
     3181 ttgcctgata ttaacacctc cttgcaaaag tcattcactt tttccttact cagtattgtt
     3241 aaatctaaaa aaggaatttc gatttattaa tttcacatca ataattataa catgggtaaa
     3301 ttatcaagtc atttcttttc tgatttcgta gataatactt atctccatat atttctactc
     3361 gtagtcgttg tacaaatcgt tgtaaatcat gctaccagac ctcgcatttc ctgcatctga
     3421 cgcatgagag tataagtcca gcagtcttgc ttcctgtact ttacttaatg cggttttgag
     3481 cctactgtcg tcctcaataa cttcgcttag ttggaaatca aaaaggttct tccttacata
     3541 gttgtcctcg attgccatgt agaatgacac ttttaaggaa cgcttgctgt attataaaaa
     3601 gcagagttac ttcatatttt cttacgtgtc aaaaagattc caataattgc tcctataagg
     3661 acaatcggta ctaatctgac aaatagccac tcaaaagcat gaaaagctac tccaaaaaca
     3721 gcagtttcag ggtcggtata attccaatcc aagtaaggac tatttagtcc ccctaagacc
     3781 acaaaaacta ttaatatgac tgcacataat aaaataaata accaatgaaa catttttctt
     3841 ctcatgtttt ttctttgtgc aagttgcaag tttataatct ctaatttttc agaaatcact
     3901 tttaagctat caacttccga ctcaacaata gtttctccaa gtaaagtgct tacgggcgtt
     3961 tcaagcgcct ctgatatgga aatcaacata tcagaatcgg gaaccgacag tccttgttcc
     4021 catttagata ttgtttgtcg cactacattc agtttgatag caagttcttg ttgtgaaagt
     4081 ccttttgact tcctgattgt tttaatgttt tcgcttaaca ttagggctaa ccacctttct
     4141 ttcagttgtt accgatatta taggcaaaac tgcccattgc ttcaagcaac gcattttaac
     4201 attcaagtaa atttcttctt tttcctttaa ccgttttgct tatatatagt ttttatgagg
     4261 atattctcca tttttaatcg ctttttataa taaaaataga tatgtttact aattaaaact
     4321 gtagaaatat ttataaaggc ataagacaaa tttacctaca atttttagtg tttagattct
     4381 aaaaaaataa aaataattat aattgagttc ataattttaa aacttattga atataattat
     4441 atgtcggtta aagacgtatg aaaataaaag gaatttacaa gttatgtgtt gtatagatat
     4501 tgtatatgca gcatgtaata aatcttagca ctatttatat tctatacaaa aagatatttg
     4561 aagggagatt aaaaatggga atattagaag gcgctgcaat gataagagag attgcaatta
     4621 aaatagctaa agaaaaaggt attacagaac aaaaggcatg gcccgaagct gtaaaagaat
     4681 ttaaagaaaa atatgaactt gtattataaa ctttgactgt aaaactgtct ctattttatt
     4741 gagacagttt tttatattgt aacattcata ttacgaagaa agatatatat aatcttagat
     4801 tccttgtaac tttaatttaa aatgaaataa tttagactaa aaacagtatt aataaatttt
     4861 atttatcgta taaatttgtt tttaatatta tttttaaggt ataaatctta tatattagat
     4921 ataaattttg gaggtatatg atttgaataa aaaacttgaa aatagatgct catggcaaga
     4981 atattttatg agactgtgcg aaacagtagc tgaaagagga acttgtgata gagcttatgt
     5041 aggagctata attgtcaatt cagaaaacag aatagtgtct actggatata atggtagtat
     5101 ttctggagat aagcattgta gtgaagttgg tcatgaaatg agagatggac attgtattag
     5161 aaccattcac gctgaacaaa atgcccttta ttattgtgca aaagaaggta tttcagttaa
     5221 agattgctct atttatgtaa ctcactttcc ttgcttaaat tgtactaagg ctataattca
     5281 agctggaatt aaacatatat attatagaac tggatataga atagatgaat atgctataaa
     5341 attattgcaa tcaagtaatg tcttatatac aaaattataa ttttaacatt atttacttaa
     5401 tattctatat ttttacaaaa tgggttataa taaaagtata agatataaaa agaaacaaaa
     5461 ttctaggtta tgcatatatt aaatagaagt taaatttaat tgtaaaggag atgttagttt
     5521 gaaagtagta ataataccag ggcacacttt aattggaaaa ggtacaggag ctgttgggta
     5581 tataaacgaa agcaaagaaa caagaatact gaatgactta attgttaaat ggcttaaaat
     5641 aggtggagct actgtttata caggaagagt tgatgaatca agtaatcatt tagcagacca
     5701 atgtgctata gcaaataaac aggaaactga tttggctgtt cagattcatt ttaattcaaa
     5761 tgctacaact tcaactccag tagggacaga aacaatatat aagactaata atggcaaaac
     5821 ctatgcagaa agagtgaata ctaggttagc tacagtattc aaagataggg gtgcaaagtc
     5881 tgatgttaga ggtctttatt ggcttaatca tactattgca ccagcaatat taatagaagt
     5941 atgttttgta gatagtaaag cagatacaga ttattatgtt aataataaag ataaagttgc
     6001 taaattgata gcagaaggta tactaaacaa atctatatca aattctcaag ggggagggga
     6061 aaataaagtg tatgaaaatg taatcgttta tacaggtgat gctgataaag tggctgctca
     6121 gattttaaat tggcaattga aagattcttt aataattgag gcgtctagtt ataagcaagg
     6181 tttaggtaaa aaggtttatg ttgtaggtgg agaagctaat aaacttgtaa aaggtgatgt
     6241 agtaataaat ggtgctgata gatatgaaac tgttaaatta gcattacaag aaatagataa
     6301 attataaaat catgattatt ttataaaaaa taaataagtt aggataaaaa aatgataaga
     6361 acaaaagttg tagagttgat agcaacagta tgcagagaaa acaaaccaca taaatgggtt
     6421 gatgaaaatt atactcctta tgataaaagt ggtaaagttg aattaatgtc aatagaagat
     6481 ttaaatgaat taatatcaag cagtggaaga gcagattttc tttatagcag tagattacaa
     6541 aagcttctag aggaggttta tataaatcaa agtagagcat cttatatatc aggttgtgga
     6601 ttattttgga gttcgtattg ggatatactt gaagaaaaat ttgaagaatg gctatataat
     6661 tcttatatct tttttgatga agatgatgaa tatttagaag gaatggaaga ttttgagctt
     6721 gaatgtaaag atgttcttat ggatgtaatt gaaacaacaa gcatagatat atatgttcaa
     6781 atgataaaga ggaatattac caattattaa aaacacatag tgtcaaatca agctaataaa
     6841 aaacgacctt ataaatgcta ttttaaggtc gtttaaatta tatcttatag gcttatttta
     6901 tattaactta ttatttttta tctagaaaaa tgtttttaat tatagtcaat atatcaacta
     6961 taacatccat atttgatttg acactgtctt tagtaacaag aaaatctgct gtaacttcag
     7021 taactacttc tgttacatct ttcatatttt ctcctacttc tccaaaagtt tctacaacat
     7081 taggaagatt atttaaagtt gtatttatat tttgagtatt ttcatttaac atagttccaa
     7141 cattatttaa aatcttatgt agtttaacaa gtacaataat taaataaact aaagcaatac
     7201 aagcaagtat agctaataat acatatatta tacttatgtt aatcgttatt gtattgtcca
     7261 tattattcct cagtaacact aactggttca actatatcta ttattttatc ttcctcacta
     7321 gaattttcaa cactcttttt gttatctata tattttttta tttttttctt tgattctata
     7381 aagtttcctt ttttatcctc tagcttctca gatactttgc cttttacatc tacagttttc
     7441 atatttatct tttcagcaat ttgttgtgaa gcatctttta tatcctctct tgtttctttt
     7501 ccagatttag gagccaaaag taatccactc actgcaccag taacagctcc taataccaca
     7561 ccagctgtag caattttagc tttcttaact ttttcagctc tctgtttagc ttttctttta
     7621 tcttctattt tcttcgatag attcatgttt aatcgctcct tattataata tttctaaaca
     7681 ttataataca ttttttacca taatgatata gtttctaact aaatttttaa aaatcaataa
     7741 aaattc
//
LOCUS       DAFKMX010000174         7701 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFKMX010000174 DAFKMX010000000
VERSION     DAFKMX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.176"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            950..2308
                     /locus_tag="LZB08_003083"
     CDS             950..2308
                     /locus_tag="LZB08_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBM7316564.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2415..3209
                     /locus_tag="LZB08_003084"
     CDS             2415..3209
                     /locus_tag="LZB08_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBM7316565.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3202..5508
                     /locus_tag="LZB08_003085"
     CDS             3202..5508
                     /locus_tag="LZB08_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7316566.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPVFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5647..6924
                     /locus_tag="LZB08_003086"
     CDS             5647..6924
                     /locus_tag="LZB08_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7316567.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7107..>7701
                     /gene="yqeC"
                     /locus_tag="LZB08_003087"
     CDS             7107..>7701
                     /gene="yqeC"
                     /locus_tag="LZB08_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBM7316568.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLF"
BASE COUNT         3013 a          839 c         1444 g         2405 t
ORIGIN      
        1 gtatatttta tgttgcagat acaaatatag taaaggagag tacattggta caactgagat
       61 acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat tcataaatat
      121 tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta attaatttaa
      181 taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca aagtatgtta
      241 aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca actttaaaac
      301 cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt tatgaatgtg
      361 gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta cttaaaaatg
      421 gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga gagagagtac
      481 actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg aagatgaaga
      541 aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca tctataaagt
      601 agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa aacttataac
      661 ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa aaattaaaaa
      721 ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata caaaattaaa
      781 gaatacgttt atttgttaat aattaaattt attgcatctg ttttttaaga ctaaaaactt
      841 aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata tattagattt
      901 ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta tgaaagcaaa
      961 aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa tacctttagg
     1021 gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat tgattgcaaa
     1081 tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat tagtttttgc
     1141 aggattatca actttattac aattatatcc aatacctttg tttaaaggtt ttaaattagg
     1201 aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg gggtatctgt
     1261 tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg gtatagtaat
     1321 aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag ctgtatcagg
     1381 aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa acttagctgg
     1441 gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa tatctgtatc
     1501 tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg catcaatatt
     1561 agttagcata atttttggat acattttatc attaattttg ggtatagtaa atttttcttc
     1621 aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc tagatataag
     1681 acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg atataatggg
     1741 tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg aagaactttc
     1801 ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt ctgcaactcc
     1861 aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg taagtaaatt
     1921 tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt tattagctaa
     1981 tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta tattttcaac
     2041 tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag aaaatagcat
     2101 gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc aagttcttga
     2161 aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt caggagcaat
     2221 ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata aaagtgataa
     2281 ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga attaaatata
     2341 ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta ataagttaat
     2401 gatgggggga taagatggtt actataaagg aatattcagt tccacagagt ttagaagaag
     2461 catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt ggatttataa
     2521 aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat ctaaattata
     2581 taaaagagga tgaaaaaaat attttgatag gtgcagatac ttcacttaga agtttagaaa
     2641 ttgataaaac tataaaaaat tactgtagtg gaatactttc aagcgctgtt tctaatatag
     2701 ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca aagtatggct
     2761 tttctgactt aataccagcg cttttggttg ttggagcgaa ggtgagatta tacaataaag
     2821 gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata ttggtcgaag
     2881 taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa tgcacaggag
     2941 atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa atagcaattg
     3001 gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta aataaagaaa
     3061 aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga agtaatatga
     3121 gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg atgtataatg
     3181 ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga tagtacagca
     3241 attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga tgttttgaca
     3301 ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga tacatcaaaa
     3361 gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc acaggtcaga
     3421 tattgtgttc caggtcaaac agaaccagaa gcatcacctt atgatagagt tttacttgaa
     3481 gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga tgagaaaaca
     3541 gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc tgtgtttgat
     3601 gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat agtagagcca
     3661 tacccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt tgaatttgga
     3721 gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata caaaacccaa
     3781 agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga tataaatgat
     3841 agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat acttagaaaa
     3901 gtattgggta taccgtcttc aaaaataaga gtaattaaac ctagaatagg tggtggattt
     3961 ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat gaaaactggt
     4021 agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa tagtagacat
     4081 gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa agcaatagag
     4141 ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt ttcttccgaa
     4201 tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt taatgctaga
     4261 acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc tacacaggga
     4321 atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat ggacccactt
     4381 gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc tgaagacgaa
     4441 atattaagtt gtgagctgag acaatgtata gaaaagggca agaaattaat taattgggat
     4501 gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg tatggcagta
     4561 gcaaatcatg gttctggtat accacgtgta gatatggcaa cagtcactat aagaatggat
     4621 gaagatggca catataaact attatctggt cttgctgact taggtcaagg ggctgataca
     4681 attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat ttctatttat
     4741 acaggtgatt cagatttatg tccctatgat acaggagcat ttgcttcttg tacaacaaca
     4801 gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt actttcagtt
     4861 gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga cagggttttt
     4921 gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga atctgttgga
     4981 ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga atcttttggc
     5041 atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt agataaaaat
     5101 acaggcgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac agttataaat
     5161 ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg gtttgcaatg
     5221 tatgaagacc ctgtatttga taaagatggg agattattaa cagatagttt cctacagtat
     5281 aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga gaatttcgac
     5341 ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac accagcacca
     5401 gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct tcctatgact
     5461 tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata aataataagg
     5521 tgtataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa tggacattat
     5581 atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc taaaggaggt
     5641 gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca acaattaaaa
     5701 aacttaacta aaaaagaata tcaagctcta cgagagatgt gcttcttagc taagaactta
     5761 tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga acatcttaac
     5821 tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct caatagtaat
     5881 atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt tgggttaatt
     5941 aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa atatttagat
     6001 aaagaagggt tttttagcat aataataggt caaataagaa tcaaagaaga tgggatttta
     6061 aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat aaaagttcca
     6121 caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca taaagctagg
     6181 ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc aaatcaaaaa
     6241 aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac caatttaggt
     6301 gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc aaacaaacag
     6361 aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac aagaaaacaa
     6421 tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa aacctgtttt
     6481 tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg atataatgct
     6541 aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat aagaatccca
     6601 tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat taattttatt
     6661 aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt tctacctgat
     6721 atcaatgtgg acaatgttaa aaaatatgac tttaaagggg aaagaataac acgtggacag
     6781 tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagtct taatatacta
     6841 aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat attaaagcaa
     6901 ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc gtaagcttac
     6961 agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt actttcgagt
     7021 ttatggaatt tcaaaaatca cttttactaa aaaggtctaa ctataataat taagagatta
     7081 gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa aagaatgaaa
     7141 taataactgt agttggagca ggaggaaaaa cttcgtttat aaactatttt gcaaattttt
     7201 atagagataa attgaaagtt ctccttacaa caacaactaa aatatatgta ccaaacgatt
     7261 atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt catggaataa
     7321 cagtatgtgg cagttatata aataatgaaa ataaattagt aagtattgat tcctctattt
     7381 tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc tcaaagagaa
     7441 aaaaacttaa aggttggaat gctaaagaac cagtagtata tcataaaact acaaaaacca
     7501 tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat atacatagag
     7561 tagaaatatt taagaaaata gcaaatttag atacaagtag tattaatagt agttctacag
     7621 taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt aaaaattatt
     7681 ctggaaaaag agtattattt a
//
LOCUS       DAFKMX010000175         7633 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFKMX010000175 DAFKMX010000000
VERSION     DAFKMX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.177"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            18..92
                     /locus_tag="LZB08_003088"
     tRNA            18..92
                     /locus_tag="LZB08_003088"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gly,seq:gcc)
     gene            117..193
                     /locus_tag="LZB08_003089"
     tRNA            117..193
                     /locus_tag="LZB08_003089"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Arg,seq:tct)
     gene            203..278
                     /locus_tag="LZB08_003090"
     tRNA            203..278
                     /locus_tag="LZB08_003090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Gln,seq:ttg)
     gene            287..362
                     /locus_tag="LZB08_003091"
     tRNA            287..362
                     /locus_tag="LZB08_003091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Lys,seq:ttt)
     gene            365..453
                     /locus_tag="LZB08_003092"
     tRNA            365..453
                     /locus_tag="LZB08_003092"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Ser,seq:tga)
     gene            457..532
                     /locus_tag="LZB08_003093"
     tRNA            457..532
                     /locus_tag="LZB08_003093"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            539..612
                     /locus_tag="LZB08_003094"
     tRNA            539..612
                     /locus_tag="LZB08_003094"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Met,seq:cat)
     gene            627..703
                     /locus_tag="LZB08_003095"
     tRNA            627..703
                     /locus_tag="LZB08_003095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Ile,seq:cat)
     gene            721..797
                     /locus_tag="LZB08_003096"
     tRNA            721..797
                     /locus_tag="LZB08_003096"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:His,seq:gtg)
     gene            806..881
                     /locus_tag="LZB08_003097"
     tRNA            806..881
                     /locus_tag="LZB08_003097"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Lys,seq:ttt)
     gene            889..962
                     /locus_tag="LZB08_003098"
     tRNA            889..962
                     /locus_tag="LZB08_003098"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Cys,seq:gca)
     gene            970..1046
                     /locus_tag="LZB08_003099"
     tRNA            970..1046
                     /locus_tag="LZB08_003099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Arg,seq:acg)
     gene            1059..1134
                     /locus_tag="LZB08_003100"
     tRNA            1059..1134
                     /locus_tag="LZB08_003100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Val,seq:tac)
     gene            1210..1623
                     /locus_tag="LZB08_003101"
     CDS             1210..1623
                     /locus_tag="LZB08_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316569.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1932..5363
                     /locus_tag="LZB08_003102"
     CDS             1932..5363
                     /locus_tag="LZB08_003102"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBM7316570.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5605..7533
                     /locus_tag="LZB08_003103"
     CDS             5605..7533
                     /locus_tag="LZB08_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBM7316571.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3031 a          938 c         1517 g         2147 t
ORIGIN      
        1 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
       61 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      121 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      181 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      241 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      301 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      361 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      421 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      481 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
      541 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
      601 ctattaccgc tactgaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
      661 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
      721 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
      781 tctcattatc caccccaact tatatggtct attagctcag tcggtagagc acctgacttt
      841 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
      901 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
      961 caataaaatg cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag
     1021 gggttcgagt ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag
     1081 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa
     1141 acctctagta aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag
     1201 gtaaaaaatt tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata
     1261 attatagata aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga
     1321 gaagatgtaa tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat
     1381 gagcaatgta atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg
     1441 tctgatgaac ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct
     1501 agagcatata aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat
     1561 gcagatttag gacacttaaa actttctgat gtagtaaaga ggctatctag acaagtagat
     1621 taggatatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
     1681 aattatgtaa acatagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat
     1741 aaattcatct gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca
     1801 cagtctaaaa attacttata tttataaatt tatcttatta attaaggaac tttcttaaac
     1861 atcttcaaaa ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag
     1921 aagaatagag tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag
     1981 ctataagaat atttagagcc tgttcagaat tggggataaa aagtgttggt atatacagta
     2041 aggaagataa gtatggattg tttagaacaa aagcggatga atcatatttg ataggagaag
     2101 gtaaagggcc aatagatgca tatttagata tggatggaat aatagaccta gcaaagagaa
     2161 aaaaagttga tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc
     2221 gtaaatgtga agagaatgga attactttta taggaccatc atctaaggta atgaatatga
     2281 tgggggataa gattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag
     2341 gagtagaaaa agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggat
     2401 atcctgttat gataaaagca tctaatggcg gtggcggtag aggtatgaga attgtacatc
     2461 gcgaagaaga cttagagctt gaatatgaaa ctgcttgtag tgaatcaaga aaagcttttg
     2521 gagaagatat tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa
     2581 tattaggtga taattatgga aatatagttc acttatatga aagagattgt tcagttcaaa
     2641 gaagacatca aaaaataata gaatatgcac cagctttttc gttagatgat aaagtaagaa
     2701 aagagatatg tgaagatgca gttaagttat caaaacatgt gggttattct aatgcgggaa
     2761 cattagaatt tttagtagat gcaaatggtg ggcattattt tatagagatg aacacaagag
     2821 tacaggttga acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa
     2881 tactaatagc ccaaggttac agcttagata gtgaagaaat taatataaaa tcacaagatg
     2941 atgtggaaat aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata
     3001 aattcatgcc tgatacaggt aaaattcaag tttatagaac tggttcagga tttggtataa
     3061 gactagatgg aggaaatgga tttacagggg caaacataag tcctcattat gatagtttat
     3121 tggttaagac aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat
     3181 ctataaaaga acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt
     3241 taaataatcc tatattctca aatggtaaat gtagtactaa gtttatagat gaaaatccag
     3301 atttatttga aataacagaa agtaaagaca gaggaacaaa attacttcaa tttataggag
     3361 atgtaattgt aaatgataat gcttgtaaag aaaaaccatt atttgatgca ttacatgacc
     3421 caagaatgga taaggatggt tcaaaatcag aaggaagtaa aatacttttc gataagttag
     3481 gtaaatctgc atatatagaa aagataaaaa atgataagaa attactatta acagatacaa
     3541 caatgagaga tgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa
     3601 aagctgctaa gccaacagaa aaatatcaaa aggatttatt ctcacttgaa atgtggggag
     3661 gagctactta tgatgttgca tatagatttt taaaagaatc tccttggcgt agattacaaa
     3721 agctaagaga agaaatacca agcataatgt tccaaatgtt gcttagagca tcaaatggag
     3781 ttggatataa aaactatcca gataatgtaa tagaagaatt tacaaaagaa tctgctagac
     3841 aaggtataga tgtatttaga atatttgatt cattaaactg ggttgaaaat atgaaaccat
     3901 ctattaatac agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg
     3961 atatactaga taagactaaa actaaataca atctagaata ttatattaaa atggcacaag
     4021 aattagaaag tttgggagca gatataatag caataaaaga tatgtcaggt cttcttaaac
     4081 catattcagc ttacacacta gttaaagagt taaagaaaaa tgtaaaagca ccaatacatt
     4141 tacacactca tgatacaagt ggaaatggtg tagcaacttg tttaatggcg tcagaagctg
     4201 gagtagacat aattgatgct gcattagaat ctatggcagg acttacaagt cagccatcac
     4261 ttaatgctat agtagaggca cttaagaata cagagagaga tacaggaata gatttatttg
     4321 gatatgatga gttaggaaaa tattataagg atttaagaaa agtttataat aaatttgaaa
     4381 gtgatttaac taattcttgt gcagaaatat ataattttga aataccagga ggccaatata
     4441 caaacttaaa acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag
     4501 aaaaatataa agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa
     4561 aagttgtagg agacttagct atctttatga ctaaaaataa attagataaa gataatataa
     4621 tagaagaagg taaaaattta tcattcccag attctgttgt ggattactgt aaaggtatga
     4681 ttggtcaacc agaaggtgga ataccaaaag atttacaaga ggtagttcta aaaggtgaag
     4741 aagctataac agtaagacca ggttcattat taccagcaga agattttgat gaaatagcta
     4801 aatatctaaa tgaaaagtat gatataaatg ctaatataag aaatgttata agttatgctt
     4861 tatatccaaa agtatatgaa gattatatca aacatctaca acattataat gatatatcta
     4921 aacttgaaag tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtagaaa
     4981 tagaagaagg taaggtatta acaataagat tagttgaaat tggtgaagtt aaggaaaatg
     5041 gatttagaac tataggattt gagttaaatg gaatggttag agaagtagaa ataaaagata
     5101 agaatttctc tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa
     5161 ttggagcaag cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa
     5221 aagcaaatca accattaatc gttatagagg ctatgaagat ggaaactata atagttgcta
     5281 agactgatgg agtaattaag tcaatcaaag taaaagaaga tgatatggtt gaagataaac
     5341 aattattaat gataatgaag taatttaatt aaaataaatt aaaaagcact ctgaaatgtt
     5401 taataaaaaa tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag
     5461 aggacaatta cctccagatg tagaaataaa gctagtaagg aaaatgttat ctaaaaattc
     5521 taaatatagt aaatattttc cataatattt cttaggaaat aacagcaaag ataaaataca
     5581 taaatttaaa aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta
     5641 gcggtattag gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca
     5701 gcaaacacta ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga
     5761 ttaataccta tagagtataa tgattataag tttaacttat tggatacccc aggatatttt
     5821 gactttagcg gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata
     5881 gatgcaacag cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt
     5941 cctaagataa tgtttataaa taaaatagat aatgaaaagg caagatacaa agatgcaata
     6001 gcaatgttaa gagaaaaata caataataag atagttccta tgataagtcc tatatacaaa
     6061 gataaaaact ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa
     6121 tttaaggaac aagctatgag tgttaaagaa gcattaatgg aacttatagc agaaactgat
     6181 gaccaaatat tagataaata ttttaatgga gaagaactaa ctacagaaga gatacaaaaa
     6241 ggtattatta taggtataca aagaggtgat attatacctg taatatgtgg ttcaactata
     6301 aataatatag gcacaaagga aatattagat acaatatcaa gttatttaga acctatcttt
     6361 acagaggaaa gcaaaccatt taggggatta gtatttaaaa ctatggttga cccatttgtt
     6421 ggaaagatgt catatataaa aattactgag ggtgtacttt ctaaggataa agatgttttt
     6481 aatataaata aaaatgtaaa agaaaaaata gctaatatat atacattgag aaatagtgaa
     6541 ttagtagaaa tagaaaaagc taaagctggt gatatagtag ttataactaa agttaattct
     6601 ctaaaaacag gagacactat atctgcagat aaagatgcag aagctttaga aaagatagat
     6661 tttcctaaac cacaaatata ttatgcagta actcctaaga ataaaggtga tgaagaaaaa
     6721 gtagcatctg tattaaataa actagtagaa gaggacccta cattacattg gtatagaaat
     6781 acagagacaa aacaagcact tttaggaggt caaggagaac ttcatataaa aacaattaaa
     6841 aataaaatga aagataagtt tggtgttgat gttgaattga atgaccttaa agttccttat
     6901 agagaaacta taaaaggaac agctgatgta caaggaaaac ataaaaagca atcaggtggt
     6961 catggtcaat atggagacgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt
     7021 acagaagaaa tatttggtgg ttctgtacca aaacaatata taccagcagt tgaaaaaggt
     7081 ttaaaagact ctatgcaaaa aggtatatta gcaggatatc ctgttacaaa cataaaagct
     7141 acgctttatg atggttcata ccatgatgta gactcttctg aaatggcctt taaaatggca
     7201 gcaagtgcag cctttaaaaa aggtatggaa gaagcacacc caatactact agaacctata
     7261 atgaaactta agataactgt tccagaagaa tatatgggag atgtcatggg tgatataaat
     7321 aagagaagag gaaaaatatt tggaatggaa ccagatgaca aagggaaaca aataatattt
     7381 gcagaagctc cacaagcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa
     7441 ggtagaggat actttgaaat ggaattagag agatatggag aggttccatc acagtttgca
     7501 gaaaaaataa ttgggctagc tactgcaaag taatgaacaa aataaataga aaaataatta
     7561 atatatatta ttaaaaatat ttttagggtt ttaactaggt ataggtttat gttaggtaaa
     7621 cttataccta gtt
//
LOCUS       DAFKMX010000176         7604 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFKMX010000176 DAFKMX010000000
VERSION     DAFKMX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.178"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            5..316
                     /locus_tag="LZB08_003104"
     CDS             5..316
                     /locus_tag="LZB08_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBM7316572.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      409..501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            601..1395
                     /gene="thiD"
                     /locus_tag="LZB08_003105"
     CDS             601..1395
                     /gene="thiD"
                     /locus_tag="LZB08_003105"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBM7316573.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1419..2207
                     /gene="thiM"
                     /locus_tag="LZB08_003106"
     CDS             1419..2207
                     /gene="thiM"
                     /locus_tag="LZB08_003106"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBM7316574.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2230..2862
                     /gene="thiE"
                     /locus_tag="LZB08_003107"
     CDS             2230..2862
                     /gene="thiE"
                     /locus_tag="LZB08_003107"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBM7316575.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3001..3303
                     /locus_tag="LZB08_003108"
     CDS             3001..3303
                     /locus_tag="LZB08_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7316576.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3369..4118)
                     /locus_tag="LZB08_003109"
     CDS             complement(3369..4118)
                     /locus_tag="LZB08_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316577.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4112..5074)
                     /locus_tag="LZB08_003110"
     CDS             complement(4112..5074)
                     /locus_tag="LZB08_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316578.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5078..5536)
                     /locus_tag="LZB08_003111"
     CDS             complement(5078..5536)
                     /locus_tag="LZB08_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBM7316579.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKVQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5892..6248
                     /locus_tag="LZB08_003112"
     CDS             5892..6248
                     /locus_tag="LZB08_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316580.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6320..7036
                     /locus_tag="LZB08_003113"
     CDS             6320..7036
                     /locus_tag="LZB08_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316581.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7029..>7604
                     /locus_tag="LZB08_003114"
     CDS             7029..>7604
                     /locus_tag="LZB08_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316582.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIV"
BASE COUNT         2960 a          891 c         1250 g         2503 t
ORIGIN      
        1 taaggtgtat tttacatata ttataagatg taaagatgat tctctataca ctggatatac
       61 cagtaatata gttagaagaa tgaatgaaca taaattagga attaattcaa aatacacaag
      121 agcaaaggga tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa gcaatgctat
      181 gaagttagaa tattatataa aaaaattaac tagaaataaa aaattgtcta taataaaaaa
      241 cccaagtata cttataaatt taattgataa taaagaagat tatattattg gtaaggaaat
      301 agaacaatta acataaatat ttatagtaac attataatgg gtgcatatgg tgtaaaaact
      361 tgacaataaa tactattgta atataatgaa gttataaata tagtaaatta aaatagctag
      421 gggagccaag agctgagagg tataccgacc cttacacctg atctggataa taccagcgta
      481 ggaaagctta gtaaaaattc aagtatgtat tatttgtgat atatgctatt tctaagctat
      541 acctattgta ggtatagctt ttttaattaa gttatttaaa ttaaacttta ggaggcaaca
      601 atgagtaatt ataaaatacc aacacttaca atagccggct ctgattcatc tggaggagct
      661 gggattcagg ctgatttgaa aacgtttagt gcaattggta cttatggtat gagtgtaata
      721 acagctataa cagcacagaa tactaaagga gtttttgctg tagaggattt aaataagaaa
      781 ataataaaaa aacaaataga ggcagtcttt gaagatatac ctccaagagc tgtaaaaata
      841 ggaatggttt caagtcctga gattatactt gaaatagttg agaatctaaa gaaatacaat
      901 ccaaaatatt tggttgtgga cccagtaatg atatctaaaa gtggatatta tctattaaag
      961 ccagaggcaa aagaaaactt aattaagtat ttgataccac ttgcatacat aattacacca
     1021 aatataccag aggcagaaga aataacagga ataaaaatac ataatgtaga tgatatgaag
     1081 agagtaggag aagaaatact acaacttgga cctaaatttg tacttatgaa agggggtcat
     1141 ttagatggag aagcagtgga tattttagtt ggtaaaaata tatttaaagt ttataaaagt
     1201 gaaagaattg ataaaaaaaa tacacatgga acagggtgca cattatcttc tgcaataaca
     1261 tcttatctag cattaggata tgaaataaca gaagcagtaa atctaagtaa aatctatata
     1321 acagaagcaa taaaaagaag ttttgatata ggtcatggag taggaccagt acatcatttt
     1381 tataaatttg agtaataaaa aaatattgga ggtagaatat gtataattta attaaagatg
     1441 taaaaaaatt aaatccattg gtaattcatt atactaataa tgtaactata aatgattgtg
     1501 ctaatgtaac tttagctgta ggagcaagtc ctcttatgag tttttcatat gaggaagtag
     1561 aagaaatggt aagtgtagca aactctgtag taataaatat aggaactatg aactcaaata
     1621 tgttggattt attcctatta gctggaaaag cagcaaataa gtacaataaa cctgttgttt
     1681 tggacccagt tggagtattt gcaagcaaag cgagagcaga acttacaagt agacttttaa
     1741 atgaagtcaa atttagtgtt gttaaaggga atgtatcaga aataaagttt ataggtggct
     1801 ttaatgtaag gggtaaaggt gtagattcat ttgatgaaga agaagattct actgagataa
     1861 taagaaagat tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa atagatataa
     1921 taactaatgg taaaggtaca tataaaataa acaatggaac tgataaatta aaaggaatta
     1981 caggaactgg atgtatgact gctagcttga ttgcaagttt tatggctgtt actgagaata
     2041 tattagaagc agcaacaatg ggtgttctta caatgtcttt aagtggggaa ttagctaatt
     2101 taaataatcc accaataggt acatttaaag aaaatcttat gaatgctatt tatcaaatgg
     2161 atatagatac tttaagtaaa aattctaata ttgaattttt aaattaataa agagtaaggg
     2221 agacttaaaa tgattgataa agaatcttta aaaaaatgtt taaagttgta tttagtaact
     2281 gactctgaaa tgcttaaagg tagagacttt tataaatgtc ttgaagatgc aatatcttct
     2341 ggaataacaa ctgtacaact tagagaaaaa aatgcttctg gaagagagtt tttaagaaaa
     2401 gctatgaaac ttagagaaat aactaaacga tatggtgtta agtttataat aaatgataga
     2461 gtggatatag cacttatttg tgatgcagat ggagtacatg taggacagag tgacattgat
     2521 gttagagagg ttagaaagct tataggaaac aataaaattc taggagtatc agcaagaact
     2581 ttagaagaag ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt agggtctata
     2641 ttcacaacat ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt taaagaaatt
     2701 aaagaaaaag tagacatgcc atttgtacta attggaggta taaatttaga caatatagat
     2761 aaacttaaat gtttagaaag tgacgggtat gctataatat cagctatatt aaaagctgaa
     2821 gatatctcta aagaagtgga aaagtggaca ttaaaaatat aaaattggct ctggtagtga
     2881 aaaaaatgga aaaatttgat ataatctaag agtaaaattt aagcaattat aaatgataaa
     2941 cttttctaca tatacttttt aatcaagata tatgtagatt tggttaaaaa ggagctataa
     3001 atgattagga tattgttagt gtgtgttgga ggcatgtctt ctaccctatt ggtaaataaa
     3061 atggagaagg atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt tggagaagga
     3121 gatataaaat cggagcttga taactttgat attttacttc ttggaccaca acttagattt
     3181 atgcttgatg acgtaaagtc tatagttgga gatagagctc cagtatctat aatagatatg
     3241 gtaaactatg gcacttgtaa tggtcatgct gtgttaaatt cggttttaga aatattaaaa
     3301 taaataataa aagtggattc tatataatag agtccatttt tattatttgt tattaatagg
     3361 cttaaaactt agcttaccat ttcttttttt aatctccaca tagcaagtga aaaacttgaa
     3421 cagcacaata ttaaaaatag taggataaca ggcagtatat ctccaataag aatagtctca
     3481 ccaaatatta agctacgtaa agcgttaata acatgggtaa atggattgag catcactgct
     3541 acttttaatc ctccagataa actttctata gggaaaagag ctgaacttaa aaagaaaata
     3601 ggaagtacaa ttgcagtcat aattgtttca taaactattt catttggcaa taataaactt
     3661 atagaataag ctagacttga taaaaagaag gctgtcataa atatcagtac tatcataagt
     3721 aggattccac caatgccaga ttctatcctt actgagaaga atatacttac tatacacaaa
     3781 atagttactt ctatgaaaga aactaagata gcttctaata attgaccaag gacgattgaa
     3841 tacctactga taggtgcaat taagactcta tagaaacttc cactgttctt cattataaag
     3901 tttataatac caccactact acaagaagaa aacacaacca gtacaatgat acctggtagt
     3961 ataaaagcag tataattatt gatatttata ttattcatag tttggtttgc aatagaacta
     4021 taaagcacaa gccaaagaaa aggttgtaat atagtaacta caaatgagat tggattttta
     4081 aaacgccact tcatactacg ccataagata tttaacattc ccattcctcc tttaagcttt
     4141 ttttagaact tgtaagtgct aaaaatacat cttcgagact tggctctaca atttctattg
     4201 catcaaattc tatatcatgt tctaaaagcc atttatttat taaagtaaaa gcagttctac
     4261 tatcgtttac acttataaaa attgaatttt ctcgtacgct cataaacttt actaaaccag
     4321 tacttttaat tgaatcttta tattttttta tatcttcagt attatgaaaa gttatacgta
     4381 gtatattttg tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct aaatcctttc
     4441 cattcttcat aatacaaata ttatcactca attgttcagc ttcttcaaga taatgagtag
     4501 ttagaaatat agttgtgcca tactcatctc taatttttaa taacatatcc cacatagatt
     4561 ttcttgaatc gacatccata ccaacagttg gttcatctaa aaataggatt ttaggagatg
     4621 ataccatatt catagctatg tccaatctac gttttacacc ccctgaatat gatgaagtag
     4681 gatattttaa gtaactagat aagtcaaact tatcaattaa agagtctatt ctttgttttg
     4741 caatctgtgg ttctacttta tacattttac tttgaaatac catattttcc ataagggata
     4801 aatgttcatc aatagagatt tgctgtgcta cacaagcaat ttgagtacgt atccaagaag
     4861 gattatctac taaatctttt ccaaacattg ttacatttcc agatgtaggt ttgtaaaagg
     4921 tagtcaaaat atttataaga gaagatttac cagcaccatt aggaccaagt aaagaaaaaa
     4981 tttctccagc atttacatta aagcttagac catttaaagc tttcacacca tttttatatt
     5041 cttttactaa attgtctatt tcaatcgcta acatatatta ttcctcctct ttaagtggaa
     5101 atattatctc tgtaatatag ttatctgggt ttccttttat caacatacct ggtcctttaa
     5161 taaatacttc tctccaagga gtttgtactt ttatattatt ttcatgaata tatttaaata
     5221 tcatttcata cgtttttggc aaattactat aagagcctgt gtgagtaaaa aataaagctt
     5281 ttgttttagg aaattctttt gtagttatac ccatacgatt tggagtttca gcagttggaa
     5341 cacataattc catttctgca acaccagttt tttggtcaac atcatagtag caaaagaatg
     5401 gtgcaccgtt agactttcct tttatagctt taaatacatt tggaaaatac tttgttgcct
     5461 cagtcatagg tccattataa cgcatggtag ccactgtgac tgattcgata tttttgattt
     5521 caattttgca attcatttgt ttacctccta atgtgtgttc atattcttac tagattttta
     5581 agcattaata catataagat tttgattagt tgaaaacata taatcttatc tcgtttgctt
     5641 gttttcaagt ctaacataac ataaaatata tatcctctca tttgatgctc cattttgtaa
     5701 agttattatt taagctttac tttagatttt ttagcaaatt aaaataaagc aatttataaa
     5761 ataagtattt acaaattaag tgtattaatc gtataataca cttaatagga tactagataa
     5821 agtctgaaat aaaggcgtat ttttttaaag taagtgtact atcgtattaa tacactagaa
     5881 taggaggaat catgaatttt gaattagata atacaactcc catttatttg caaatagtaa
     5941 agtatataaa gagacaaata gtaacaggag agttgaaacc aggagagaca ataccatcaa
     6001 gaagagaaat ggcacttaat ctaaaagtaa acttaaatac agttcaaaga gcatataagg
     6061 agatgggtga tatgaatata ataaatactt ttaaaaatta tcaaagcagt gtaacagttg
     6121 atgaaaatat attaaaaaat ttaaaattag agcttataaa tgaatcacta tctgttttta
     6181 tagaagatat gaaagcaata aatgttagca aagaagaagt tttaaaaata atagaagata
     6241 agtattagtt ttttaacaaa tagaaaactt agtttagaaa gtttatttat aagcatattg
     6301 gatgtggagg atagtaaata tgatagaaat aaagaatgtc agcaaaacat ataaaaggat
     6361 gcaagggctt aaaataagaa agatagaagc tttaaaaaat gtaagtttta atatagaaaa
     6421 gggaaaaata actgcattac ttggtataaa tggtgttggt aaatcaacta tgttaaaagc
     6481 aatagcagga cttataaaca ttgatagtgg agaaattcgt atagatggag aaaaaataaa
     6541 tgaaaaagta tacaataaat tggcttttgt tccagatgta caatctcact ttagcaatac
     6601 aacaataaaa gaaacatttg aatttatgga aattttttat tcaaagtgga ataaagaaaa
     6661 atcaaaagaa atgatggaca tatttaaatt ggatgaagat gaaataattg ataacttgtc
     6721 aaaaggaaac attgcaagag taaaattaat acttggattt tgccaagacc cagagtacat
     6781 acttcttgat gaaccattta caggaattga tttgtttaag agggaagagt ttataggagt
     6841 aatagcgcaa tatatggagg aaaatcaagc cattatcata actactcatg agatagtaga
     6901 gatagaaagt ttagttgatg aggttgtaat tcttgatgaa gggcagatta taacttcatt
     6961 taatgcagaa gaattaagag aaagagaagg taaatctata ttagataaga tgagggaggt
     7021 atataagaat gaataaactt ctatatctaa taaatgtaga tttgagaaga agcaataagt
     7081 tttatatagc ttatatatca tttttttcat taattacact tagtttaaat ttattcgaaa
     7141 ttaataaatt taaaaattct aaatttatta tgaacttagc aatagaagat tttggaggta
     7201 tattttatgg actagggata cttagtaatc ttggttttat ccaaagtata ttgttctttg
     7261 ggatattggg actttttata tactatgttt ttatgtggaa aagagagttc ttttcaaata
     7321 atcaaagtat ttatacactt atgatgttac ctcaaaataa gtttaagatt tgtatatcta
     7381 aatcaatagc cttgataaca atgatatatg gttttttgat aagtcagaca gcaacattat
     7441 ttatatgtaa atatatattt aatttcattt ttagaaatat gcctataatc aatatgaact
     7501 ttgccaaaga tttatattat ttaaacttga agagtatacc aattgatttt atctctttta
     7561 tggctattta tgtattttta ctacttatag ctatttctat tgta
//
LOCUS       DAFKMX010000177         7552 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFKMX010000177 DAFKMX010000000
VERSION     DAFKMX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.179"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            182..1159
                     /locus_tag="LZB08_003115"
     CDS             182..1159
                     /locus_tag="LZB08_003115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBM7316583.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1152..2120
                     /locus_tag="LZB08_003116"
     CDS             1152..2120
                     /locus_tag="LZB08_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBM7316584.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2304..2894
                     /locus_tag="LZB08_003117"
     CDS             2304..2894
                     /locus_tag="LZB08_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBM7316585.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2913..3683
                     /locus_tag="LZB08_003118"
     CDS             2913..3683
                     /locus_tag="LZB08_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBM7316586.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3820..4788
                     /gene="dusB"
                     /locus_tag="LZB08_003119"
     CDS             3820..4788
                     /gene="dusB"
                     /locus_tag="LZB08_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBM7316587.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4897..5376
                     /gene="greA"
                     /locus_tag="LZB08_003120"
     CDS             4897..5376
                     /gene="greA"
                     /locus_tag="LZB08_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBM7316588.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5391..6920
                     /gene="lysS"
                     /locus_tag="LZB08_003121"
     CDS             5391..6920
                     /gene="lysS"
                     /locus_tag="LZB08_003121"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBM7316589.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7229..7423
                     /locus_tag="LZB08_003122"
     CDS             7229..7423
                     /locus_tag="LZB08_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316590.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         3022 a          760 c         1517 g         2253 t
ORIGIN      
        1 attttaaaat tatttaaata aaatgtaaaa ttatataaat catcaatata aaatataaaa
       61 caaccattat aataattaaa aaggaaattt aaaattaata gtattaaaag tatattaata
      121 atgtataatt atttattaga agaaaataag tcatgaaatt atgtcatatt agaggtgaaa
      181 tatgcgagat aaaataatag agattatatt aaataataaa tctgaattta tttcaggaga
      241 agaattatca aaacaattag gggtgtcacg tgcagctata tggaaacata tgaaagcctt
      301 aaaagaagag ggatataata tagaatctgt aaataaaaaa gggtatcgat tagcggaaaa
      361 tccaactgat ttattaagcc cacagaatat ttactataaa ttagatacag agtttattgg
      421 aaaaaatatt gaacatcttg acactataga ttcaacaaat gaacatgcta aaaggatagc
      481 tataaatagt gtagatggga ctgtgatttt aagtgaacat caagatatgg ggaaaggacg
      541 attgggaaga agatgggatt caaaacctca tgaaggaatc tggatgagta ttatattaaa
      601 accagatatg gaaccattca aagccccatt tataacttta atagcaggag caagtgtaac
      661 gaaagcttta aacaatctag gagcaaatac attaataaaa tggccaaatg atgttatttt
      721 aaatggaaaa aaaatatgtg gtatattaac agagttatct gctgaaattg aaagaacaaa
      781 ttatgttgtt cttggaattg gaataaatgt taaaataatg gattttgaac aagaaatagc
      841 tgacaaagcc acatcattgt ataaagaagg gtatatgtta tccagagtag atattgttag
      901 gaatgtattg actgaatttg aaaaattata tttagattat actgtaaata attccaaaga
      961 aaaaacctta aaaatatgta gagattattc tgcaattata gataaaaata tatatgtatt
     1021 aaaaggagat aatagagaac tagtgaaatg tttagacata aatgaagagg gaaatttatt
     1081 agttcaaaga agtgatggaa gtataaaaga aattatttca ggtgaagttt ctataagagg
     1141 agaaaaagga tatgtataat aatattttgg atatagaagg cttaaaggtt gggcaggttg
     1201 aggatagaaa tggtcttact gggtgcacag tagtaatatg tgaagaagga gctacttgcg
     1261 gtgttgatgt aaggggctct gctccaggga ctagagaaac cgatttatta gaccctgtaa
     1321 atatggttca aaaagttcat gcagtagttt tggctggagg ttctgcatat ggtcttgaat
     1381 ccacttgtgg tgtaagtaga tacttagaag aaaaaggaat tggttttgat gtaggtgtag
     1441 gtaaagtgcc tatagttgta ggagcagttt tatttgattt aggagtaggg agctttaaat
     1501 gtagaccaga tttaaagatg ggttatagag cttgtgaagt tgcaaatact gatattttaa
     1561 atcaaggaaa ttatggagca ggttgtggag ccactgttgg aaagataaga ggtcaggaat
     1621 atgctatgaa aggcggaata ggtagctatt ctataaaatt agataatggt ctagttgtat
     1681 ctgcattaat tgctgtaaat gctcttggag atgtttatga aaatggtaag gttatagctg
     1741 gtgctttaaa tgataaaagg acagaaacaa ttgatacata taaattaatg aaacaaggtg
     1801 aaagtaaagg tggatttagt gtagataata ctacaattgg gataattgca acaaatgcta
     1861 agttaaccaa agctggatgt aaaaagatat ctcaaatggg acatgatgga tatgctaagt
     1921 ctatttttcc tatccataca cctcatgatg gagatacaat attcacaata tcaacaggag
     1981 aaatagaagc agatataaca ttagtaggtt cattggctgt agaggttgtg gaaaaaagta
     2041 taataaatgc agtaaaaaaa gcggatagtg tagaaaatat aatggcatat aaagatatag
     2101 taaaaaaacg agaaatatga tgaaattagc atatttttct tgtcaaattg ttgacaatgg
     2161 caactcatct tgataaaatt attcttgtta gagcgggcat cctacaatgg tagcttgact
     2221 catagttata agaaaaaaac gaaaaaaatt aaaaaattaa gtagtccggc atcttaatga
     2281 gctggaacgg aggtgtattt tttatgatga acacaagtgc atcagtaaga aaagtaaatg
     2341 taagaaagat gactataata ggagtactat cagctatttc tattatgcta tctatgactc
     2401 cattgggatt tattccagtt ggacctacaa aagcaactat aatgcacata ccagttatta
     2461 ttggagctat tatggaagga ccaatagttg gggcagcaat aggatttata tttgggatat
     2521 caagtctctt aaatgctata acaaacccaa cagtaacatc ttttgttttt ataaatccat
     2581 tagtttcaat attaccaaga gttatgatag gtgtattagc ttattatgta tatcagttaa
     2641 taattaaggc tacaaataag gtatacatat caggtttaat tacaggggca ataggttctc
     2701 tgttaaatac agctggagtt ttaggaatga tatatgtctt atatgctgac aagtatcttc
     2761 atgcaatggg taaaagtggt tcagcaggaa aagtaataat ggctcttgcc gcagcaaatg
     2821 gggtgccaga agcaatagta ggggctttag tagtagctgc tgttgccact gtacttaaga
     2881 aaagcaaaaa atagaaagaa ggttaatgac atatgcttct agtatttgat gttggaaata
     2941 ctaatatggt tttaggtata tataaaggtg acaaattagt taattactgg agaattaaaa
     3001 cagataggga aaaaacgtct gatgaatatg gaatcctgat aagtaaccta tttgattatg
     3061 ataatgtgaa tataagtgat attgatgatg ttataatatc atctgtagtt ccgaatgtta
     3121 tgcattctct tgaaaacttt tgtataaagt actgtaaaaa acaaccatta atagtaggtc
     3181 caggcataaa aacaggtcta aatataaaat atgataatcc aaaacaagtt ggggcagata
     3241 gaatagttaa tgctgtagca gggatagaaa agtatggagc accaagtata cttgttgatt
     3301 ttggaacagc aactacattt tgtgctatct ctgaaaaagg tgaatatttg ggtggaacaa
     3361 tagcaccagg aataaaaata tctagtgagg cgttatttca aagtgcgtct aaattaccta
     3421 gagtagaatt agctaagcca ggtatgacta tttgtaagag tactgtatca gccatgcaat
     3481 ctggaataat ttatggatat gttggtttag ttgacaaaat aataagtatg atgaagaaag
     3541 aattgaattg tgatgatgtt aaggttatag ctacaggtgg attagctaaa ctgattgctt
     3601 cagagacgaa aagtatagat tatgtagatg gttttttaac actagaagga ttgagaataa
     3661 tatatgaaaa aaaccaagaa taattaatat acagggaatt gggttatata tccaattccc
     3721 tttttatgat agatttctag ctatatgtgg tatattataa tgaggaaata agaatttaaa
     3781 gttaaatcac aaaataaagg caaaaatagg agaaaaacaa tgaaaatagg aaatttagaa
     3841 ttgaaaaata aagtttttct atcacctatg gcaggtgtaa cagaccttcc atttagatta
     3901 atttgtaaag aacagggatg tgggctttta tatacagaaa tgataaatgg taaggcactt
     3961 tgctatgatg atgaaaatac taaaaaaatg cttaagatag aagaggaaga acatcctgta
     4021 gcagttcaga tattcggttc agaacccgag tttatgggaa gagcagcaga gataatgaat
     4081 gattattcaa atgagattct cgatataaat atggggtgtc cagctccaaa agtagttaaa
     4141 aatggagatg gttcagcact tatgaaaaat cctaagcttg cagaagaggt attgagggca
     4201 gtagtgaaga actcaaaaaa gccagtgact ttaaagattc gaaaaggctg ggatgataat
     4261 agtgtaaatg ctgttgaaat agcaaagata gcagaggatt gtggaataag tgctttagca
     4321 atacatggaa gaactagaga acagttttat acaggcaaag cagactggga tataattgca
     4381 gagattaaga aaaatttgag tatacctgtt ataggaaatg gtgatgtatt tacaatagaa
     4441 gattcaataa atatgttaga caaaactggt tgtgatgcta taatgatagg taggggtgct
     4501 caaggtaacc cttggatatt taaaagaata aaccattata tgaatacagg cgagattttg
     4561 ccagagccaa ctcttaacga aaaaataagt actgccatta agcatttaaa acttgcagta
     4621 gaagaacatg gagagtatgt agctgtaagg gaaatgagaa aacacatcgc ttggtattta
     4681 aaaggactta gaaactctgc aaaattaagg gatgaaatta acaaaataga agactatcaa
     4741 gaagttgtgt cgaaattgga gtactatatg gaagactcct tgacataaaa ataatagtga
     4801 cttataatat agcgcataaa ttaaaaaaag attataaaat ttttaaaata taattaggaa
     4861 aattcctaag aaatttgtga aggagttgta tttataatgg aagaaaataa agagttttta
     4921 ttgacacaag agggatatga taaattagaa gaagaattag aaaacttaaa ggttgtaaag
     4981 agaaaagaag ttgcagagag aataaaagtt gctatatctt ttggtgattt atctgaaaat
     5041 gctgagtatg atgaagctaa aaaggaacaa gcacaagttg aagaaagaat tttaaaatta
     5101 gaaaatatgg ttagaaaagc tgttatcata gatgaaagca aaatagacct taatgttgtt
     5161 acaataggat caatagttaa agttaaggat ttagaatttg atgaagaggt tgaatatact
     5221 atagttggtt cgacagaagc agacccatat gatggtaaaa tatcaaacga atctcctgta
     5281 gggaaagcac ttttaggtag agctgcgaaa gaagttgtgg aagtgcaagt tcctgatgga
     5341 gttgctaagt ttgaaatatt agaaataaga agataatttg gaggaaaaaa atgaaaaata
     5401 atcaacaaag caatgaagaa gcgcaaattc aagaagacct tagtgaagta cttcaagtaa
     5461 gaagagataa actaaaaaaa ttacaagagt caggtagaga tccttttaaa gaaagtagat
     5521 atgatagaac tcattattct atggatataa aagataactt tgattctctt gagggtaaaa
     5581 ctacaaaaat agcaggacgt ataatgagca agagaataca agggaaagct ggctttatag
     5641 atattcaaga ccaagaggga agaatccaat cttatgtaag actagatgct ataggagagg
     5701 aagaatatag tgtattttca acatatgata ttggagatat agttggaatt gaaggagaaa
     5761 tctttaaaac taaaaaaggt gaaatatctg taaaggctaa atctgtagta ttactatgta
     5821 aatctttaca agttttacca gaaaaatatc atggattaaa agaccaagaa ctaagatata
     5881 gacaaagata tgttgactta atagtaaatc cagaagtaaa aaatgctttt ttaattagaa
     5941 caaaagcatt aaaagcatta agagcatact tagatgatag aggattttta gaagttgaga
     6001 ctccaatatt aaatactata gcaggtgggg caaatgctag accatttata actaatcata
     6061 atactttaca tataccaatg tatttaagaa tagcgaatga attgtattta aaaagactta
     6121 ttgttggtgg atttgataaa gtttatgaaa tgggaagaat gtttagaaat gaaggtatgg
     6181 atttaaaaca taatcctgaa tatactgcaa tagaattata tcaagcatat gcagactata
     6241 ctgatatgat ggaaataaca gaaaatgtta ttgcacatat ggctgaagtt gctacaggaa
     6301 gtatgatagt taactatcaa ggaacagaaa taaactttac accaccttgg aaaagaatga
     6361 gtatggaaga ttgtgttaaa gaatattctg gtgtagattt ttctactata aatacagatg
     6421 aagaggcact agaagttgct agagaaaaag gtatagagat aaaacctgga atgagaagag
     6481 gagaagtaat aaatgctttc tttgaagaat ttggagaaga taaattaatc caaccaacat
     6541 ttataactca ccatccagtt gaagtatctc cattatctaa gagaaatgtt gaagacccaa
     6601 gaagaactga tagatttgag gcatttgcta ataagtggga gttagctaat gcattttctg
     6661 aacttaatga ccctattgac caaaaaggaa gatttataga ccaattgaga aaaagagaat
     6721 taggtgatga tgaagctttt gaaatggatg aagatttctt aaaagcatta gaggtaggtc
     6781 tacctcctac aggaggacta ggaataggta tagatagagt aattatgtta ttaactaact
     6841 ctccatctat aagagatgta ttattattcc cgactatgaa acctattgat aacaatccaa
     6901 ataaagaaga agaaaactaa gaaatattag cattctacta agttttaaat aaagtttagc
     6961 tattaaatta tcaataaata gctaaatttt atttaaaatg tcagtgtgga atgcttttta
     7021 tatgcttcaa agattctttt agatatttaa tttataaatt accaacaaac gttagaaatt
     7081 gatattagtt tttgttgatt gagactttat atttagaaaa tatgattgaa agtaatttat
     7141 tatgaaaata tttagaataa tttatgagaa ggtaaaaaga ataatttatg agaatactat
     7201 atataaaaaa ttattggggg aatgtctgat gtttcaagta attaattttt tatgtttatt
     7261 ttgtgttgga tttttggttc ttgatgttat gatatatttt tgtaaggatg caatggatat
     7321 ttttgagggt acaactaatg ttaataaagt taagagtata aatgtttggg cagatagagt
     7381 gagagctaat tcatattctt atgaaaataa aattgcaaaa taggtaaaat tatatatgca
     7441 tgtattgttt gtaatatcaa gggttatatc tattagtttt aattatattt attaaaaaaa
     7501 ccaaaagtta taaccttttt taaaataaaa aacaaaaaaa gtattgacga aa
//
LOCUS       DAFKMX010000178         7523 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFKMX010000178 DAFKMX010000000
VERSION     DAFKMX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.180"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            85..2313
                     /locus_tag="LZB08_003123"
     CDS             85..2313
                     /locus_tag="LZB08_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBM7316591.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2551..2850)
                     /locus_tag="LZB08_003124"
     CDS             complement(2551..2850)
                     /locus_tag="LZB08_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBM7316592.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3056..6097
                     /locus_tag="LZB08_003125"
     CDS             3056..6097
                     /locus_tag="LZB08_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBM7316593.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6258..7523)
                     /locus_tag="LZB08_003126"
     CDS             complement(6258..7523)
                     /locus_tag="LZB08_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7316594.1"
                     /translation="MSHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQEAK
                     LVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPPMAG
                     FFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLTNDN
                     EVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSLINN
                     KFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFGNLH
                     PVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVCVRN
                     CHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAILSAI
                     LKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2781 a         1051 c         1097 g         2594 t
ORIGIN      
        1 taactttact atttgtgtaa aaagtaatat aatttaaaat tgattataaa tctaactttt
       61 ttctttatca aaatatttta ggaggtgaga catttgaaaa aaactaatag tttaaaagaa
      121 attttaaact ggaataatat caagaattta agaccttcaa agggtttaaa aaatatatta
      181 gaaattttta aaagtgacct aaaagatata cgtggcaatt atgccgtact tgtaatagtt
      241 ttaggtcttg cttttttacc atctctgtat gcttggttca atataaaggc atcttgggac
      301 ccttatggaa gcactgctaa tatgcttgta gcagtaacaa atgaagataa aggaaatgaa
      361 atagcaggac aaaagattaa tgtaggagac caattaattg aacaactcaa agataacaag
      421 agtttaggtt ggcagtttgt agatgaaaaa actgctcttg aaggtgttaa aaaaggtact
      481 tattatgcat cacttaagat tccaaaaaat tttacaaaag atttgacatc tttaattaca
      541 gatgatgtta aaaaaggcga aattatctat actgttaatg aaaaaataaa tgcagttgca
      601 ccaaagatta cagataaagg tgcttctaca atacaactac aggttaatca gactatagta
      661 aaaacagtta gtgaaatcgt tcttggtata tcaaacggag ttggtgcagg aattgaacac
      721 agtcttccaa aattgacaaa agttgaaaat tcccttattg accttcaaaa gagatttgac
      781 catataaata agactgttga tttagcttct gatgctactt caaaattatc tgatacagtt
      841 aaagatatta aatctgattt acctacaatt aaaaagactc tgaatgatac aaaactatta
      901 tcaagtgact taaaaaaatt cttagaggat acaaatgata atttagacga actttctcct
      961 cttattaagt ctgacttaaa tttaatggtt gacctttcat cttctgcttc ttctcttaca
     1021 ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg tacctaagtt gatagataat
     1081 ttatcagaaa aactttctaa tttgcaatct ttaaacgata ctttggtgga ctttttaact
     1141 aaattaaacc aacttacatc aaataataga ttggatgatg taatagataa tcttgaagac
     1201 tctagcaata aaatagattc atctatatca acattaaatg atattaaaaa caaagttata
     1261 tctggacaac aaccatctat aagtgctttg aataatgttt taagtctatc aaatggtatt
     1321 ggaagaatta atttaaatat tttaaataat tttgactcaa aaatatcaaa acctataaat
     1381 agtatatttg caaacagtat aaaagtagca aatgatataa ttactgtttt agataaggca
     1441 gaagcaaaac ttccaaaggt tgaagaaata cttactactt ctctaaaact ttcaggaaat
     1501 gcacaggaaa gtatatcatt gataagagaa agacttcctt tagcaaaagg aatgttggat
     1561 gatttgattg atactttaag caaaattagt aatggtgaag atatgaaaaa attagtcagc
     1621 ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa aagaacctgt tgaggtgaaa
     1681 acagagaaat tatatccaat tgaaaattat ggttctgcaa tgacaccatt ttatagtgtt
     1741 ctttctacat gggttggtat attacttatt tcagcactat tgtctacatc tgtacatgga
     1801 aactataaac cttatgaaat ttactttgga agaggcctta cttttttaag tatagctctt
     1861 gtccaaggtt ttataatagg aactggagat atattattgt taggagtaac agcaaaacat
     1921 ccaattttat tgatagttct gcttatgttt actagtatgg tatttaactt tatagtgtat
     1981 tctttagttt ctgtatttgg aaatgtagga aaagcaattg caatagtact attagtcgtt
     2041 cagattggtt cttctggtgg tacattccct atacaggtta cacctacatt cttccaaaga
     2101 ataaacccta taatgccatt tacctatgca ataggtgcta caagggaagc agttggtggt
     2161 atatatgctc caaatctaac taaagatata ctagttctat tactatttat gattctgtct
     2221 atttgtttaa atgtacttct aaaaggacct ataaatagag ttataaaacc atttaatgat
     2281 aaattcggcg atagtaattt aactggtcat taaaaaatta attctgtctt taaatatatt
     2341 tttttaataa tattctaagt tcatattaaa aggtagtagc taaaaagcta ctaccttttt
     2401 ccaacttaaa aactaatatt tactacaaaa actgtcatag aaaaaaacga aaaaattcgt
     2461 tctttcctaa atttatctta taaatattta gatatattcc tacttaataa gcagagctat
     2521 acatagtatt taatgtataa cttacattat ttaactcata taatcaatta ttcctgaatt
     2581 taaattatat aaatgctcaa aaccttcttc agctaacaaa ttacatgcag taacacttct
     2641 atgaccactt ctgcaataaa ctactacttt cttatcttta taatcgtaaa tttcatctat
     2701 attattgatt aactcttgta aagatatatt tatagcattt tctattttat attccttaaa
     2761 ttcatcttta gttcttacat ctaataataa aacatccttt tcatttttca tcatttcttt
     2821 aagttgcttt ttatttatat tcttatacat tgtctaaaat tcctccattt tatttatata
     2881 ttacctatac cctttattct ttatacaaca acaaatatta tttttatatt tttctctgtt
     2941 atataaaaag ctatacttat aaaagctttt tttattgata ataatctgtt gattaaaata
     3001 attaagaatg ttatgctaat tataaattat taaaaaggga aagaggtaat gtaatatgtt
     3061 taagcaaagg ctctctaaat tattatctag tacattagta ttgagtatgt tatttactgc
     3121 tgctccaaat attacttttg ctgataatac aaaagataat tccgaaaaat atcaaagttc
     3181 tgatatagaa ttacatgatt atagtaaaaa cgcggaatct tacacaaaaa ctaaagctct
     3241 tgctaaagag aaaatccaaa ctttgctatc aaagtatggt gctgtaagtg ctcaatatgc
     3301 tttgattgat aatggcaaaa ttgagatttc tggtaatggt ggagtttaca gcaaacagga
     3361 taacaaaaac ttaaataagg ataatatgta tagtatcgca tctatcagta aaatgtttac
     3421 tacaacagct gttatgaagt tggttgatga tggaaaatta aatttagata ctcctgttgt
     3481 aaagtatata cctgaattta aaatggctga tgatagatac aaggaaatca ctccaagaat
     3541 gttattaaat cattcatcag ggttgatggg aagtagtttt aaaaacacta ttttattagc
     3601 agacaatgat tcttatggtc atgataattt cttaaaagaa ttacaaaaac aaagattaaa
     3661 agctaaacca ggagcttttt ctgtttattg taatgatgga tttactttag ctgaaatttt
     3721 agtagaaaga gtatctggta tgtcatttac taattttctt gataaataca taaataatcc
     3781 acttaactta caaaatacaa aaactacaga aaatagtttt gatagttcaa aactagcaaa
     3841 agcatatgta ccttattggg aagatgcagt tccacaagat aaccttaatg ctataggtgc
     3901 aggtggttta tattcaagtg cagaaaatct atgtacattt gcacaaacat ttatgaaaaa
     3961 ttcaaatggt attctttctc ctgcttcagt aaaagctatg gaaaacaaag aatatttaaa
     4021 tggtctatgg cctgaaggag aagactctat acttggatat ggtttagggt gggattgtgt
     4081 caacacttat ccatttaatc agtataattt aaaagctctt acaaaaggtg gagactcttt
     4141 attattccac agtaatctta tagttcttcc agatgaaaat atggcagttg cagttctttc
     4201 ttctggtgga agtagtcaac ttaatgaaat aattggacaa gaaatcttac tatctgcatt
     4261 aaaagaaaaa ggtaaaatta aagaaataaa acctgataaa acatttagta aaccacaaca
     4321 agttaaaatg ccaagctctt taaaagaaaa tagtggtctt tatgcttctt caaatatgat
     4381 aaaagtagat gttaatgata atggtacttt aacagtatct tctccttata tagaaaatgg
     4441 acctgaagat aaatacgtat acataggtca agatagattt gtatctgaaa aaggtaatag
     4501 ttgtttaaaa tttgtaaaag aaaaaaataa tatcacatat ttaaatatga gctcttatga
     4561 tgatgtccct ggattaggtc agacagcaag tttatactat gtagctcaaa aagtagatga
     4621 caacaatata tcaaatagtg tcaaagaagt gtggaaaaaa agaagtggca aaggttacta
     4681 tctagtggat gaaaaatata cttctcaaag ctatatgttt ggctctgtaa aagcaagttt
     4741 tagtctttct gatgaaactc caggttatat agttaacact aaaataatgg atgagaataa
     4801 ctcaaatgct tttatagaaa tacctggtgt aataggtcgt gatttatccg acattaaact
     4861 tcataaagaa aatggtacag agtatttatc ttttggaaca ctaacgtatg ttagtgaaga
     4921 ttctataact aatttgccag cagaaaaatc ttttacttgt gaactagaat ccaatggtta
     4981 tgcaaaatgg tataagattg gtgacgatat cgcaaataaa aaaatagaag ttaatcttcc
     5041 tcaaaattca gcttttgctg tttatgatga taaaggtgtt cctgtaaact attctttagt
     5101 tacaaagaac aatagagtta gattgccaaa aggaggcgta attgttttct taggaagtcc
     5161 aaatgctaga tttgaggtta catatcaaga tgaagtaaat gcaagtgcac ttacaggaac
     5221 tgatagatat gaaacttcta ttaagataag tcaagcaggt tgggaaaatg ctgaaaatgc
     5281 agttttaata aacgattcag caattgcaga tgctttggct gcaacaccat ttgcatacaa
     5341 gaaaaacgct cctatattat tgactggaag ctctcaaata aatgaaaaaa ctttggctga
     5401 attaaagaga ttaaaagtta aaaatgtata tgtagttggt ggagaggctt ctataaatga
     5461 aaaatcttta gatactataa aatcaaacaa tatctctgta tctagaattt caggaagtga
     5521 tagatatcaa acttctatga acattgctaa agaattgaac aatattagca atatatcaaa
     5581 gatttcagta gttaatggcg aaaaaggatt agccgatgca gtaagcattg gagctgtatc
     5641 agctcaaaat gatatgccta taatacttac taatgaaaat agtaatataa ctgaaataaa
     5701 taacttgttt aaaaacaaaa agattgataa atcttatgta atcggtggag aatatacagt
     5761 ttctaaaaat atagaaagta aacttcaaaa tccacagaga atatcaggta gcactagaaa
     5821 tgaaactaat gcaaaagtaa taaaagaatt ttacaaggat tctaagatag acaatttata
     5881 tgttgctaaa aatggaatga ataaacaaga tgatttaata gacggattat ctgttggagt
     5941 tttggctgga aaaactaaat ctcctgtaat gctagtagga aactcactag attataatca
     6001 aaaagaacta tttaaaacta tgagatttaa atctgtaaca caaattggtg gaaatggaaa
     6061 tgaaaactca tttaagcaaa taaaagaaat agcttaacat aactaaaaaa ggctgtattc
     6121 aagtaatatt aattgaatat agcctttttt tatttaagtt ataagtttta tatgtgtaaa
     6181 aattaaattt aaatattaaa tgataagttt attaagatat agataagtct aaaactttgc
     6241 cttttttaag ttagtttcta tttatcattc atacttaaca atttatctaa atatactgac
     6301 tttaattgtt tactggctaa aatcttttta gctggttcaa gctttaatat agctgacagt
     6361 atagctccca tagctctatg actagccaga gcattattat caaaaattaa attttgtaac
     6421 tgcccttttt caattgccat gagaactgct ctatgtactg aatttgctgg agttattatt
     6481 ttttcatctc tctttaataa cataatgcta tttttatggc aatttctaac acaaactcca
     6541 caacctagac atctatcctc atcaattttt atatattctt taccatcttc cttaaccttg
     6601 cttatcgcat ctattggaca tgcagttata catttaccac actttacaca attttcatgg
     6661 tttatatttg gtataaaact tgttgtctgt actggatgaa gatttccaaa tcttttagct
     6721 gctaccattg cttcacagca acatccacag caattacata taaacgtaac ccctttccta
     6781 acattttctc cacattgaac taagttatgt tcatatgctt gatgaagtag ttctttacat
     6841 tctattttat ccacacgtct tgcaaattta ttatttatta aagaatttgc tacattgtca
     6901 aacgtcatac atatatccat aggagcatca caagcttttc caacatgttg cattctatgt
     6961 ctacagtaac acatacttat acctatatga gttgattcat caattatatg agtagccctt
     7021 tcataatcta aaatactaac ctcattatca ttagttaaaa cttcctcttg aacgtatact
     7081 cttccaagct tcgtttctgt tgaataaaat aaatctttga taaaatcttc ttctacattc
     7141 atatattgat agtaaagctc tgccaaaagt ttttggtcta tatcgtgtct cgttctcatc
     7201 atggcaaact caaaaaaacc agccattgga ggaggcatta tatatttctt ccctttatta
     7261 tcttctatat ctagtatcag agccttacta gcaagtttat ccaatactct atatgcttca
     7321 ctttcactta cactccaaat tttagctgct gtttttactc tgaaaggctt tattggaagc
     7381 tgagcaacta acttcgcctc ttgttctgta tatagtacat ttagtatttt gtataatgta
     7441 tcagatggtg gagcaccttg tggaaacttg tttattcttt cctctaaact tttatacata
     7501 ttcttcgcag ttatatgaga aat
//
LOCUS       DAFKMX010000179         7445 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFKMX010000179 DAFKMX010000000
VERSION     DAFKMX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.181"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            324..545
                     /locus_tag="LZB08_003127"
     CDS             324..545
                     /locus_tag="LZB08_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBM7316595.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            545..808
                     /locus_tag="LZB08_003128"
     CDS             545..808
                     /locus_tag="LZB08_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBM7316596.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            870..1442
                     /locus_tag="LZB08_003129"
     CDS             870..1442
                     /locus_tag="LZB08_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBM7316597.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            2780..3313
                     /locus_tag="LZB08_003130"
     CDS             2780..3313
                     /locus_tag="LZB08_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBM7316598.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            3539..3709
                     /locus_tag="LZB08_003131"
     CDS             3539..3709
                     /locus_tag="LZB08_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7316599.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIF"
     gene            complement(4324..4878)
                     /locus_tag="LZB08_003132"
     CDS             complement(4324..4878)
                     /locus_tag="LZB08_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBM7316600.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISGMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQIETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            5344..5757
                     /locus_tag="LZB08_003133"
     CDS             5344..5757
                     /locus_tag="LZB08_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBM7316601.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKLCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCEGIISIHDRECSECQEKVK"
     gene            5830..6366
                     /locus_tag="LZB08_003134"
     CDS             5830..6366
                     /locus_tag="LZB08_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBM7316602.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6526..7188
                     /locus_tag="LZB08_003135"
                     /pseudo
     CDS             6526..7188
                     /locus_tag="LZB08_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
BASE COUNT         2688 a          890 c         1248 g         2619 t
ORIGIN      
        1 tttataaaaa aaatagtatt aaattgtata tttatatcgt ttttaataaa aaataatcac
       61 attttattta aaaatgaata atatatagta aatagtaatt aagctataaa aaattgtata
      121 aactctatat tatttattta aaatagatat ttatgaaaga ttaattagaa aaattttaga
      181 ggtaaaataa gaaaaaaaga tacttattta atcatttaat cttattaaag acctattaaa
      241 actaccagtt ataatataga acaagtatac aatttttaaa aataatgcta ttttaaaatt
      301 ctaaaaatta aggaggataa ttaatgggat atttaaaaga ggataaacaa gactattgtt
      361 gtgaatttgc tagaccatac atcaatcgtc aaatttatga tgacacatac aatccaaaaa
      421 cagcattaaa aagaggtact ttatttcctg aattagattt aatagagtca aataactata
      481 atgattggct atatgcaaat cctaaaaata gagttagagc taaaaaaggg ggtaacatgt
      541 actaatgtca ttaaatagaa gagaactttt agagagaatt tcagaatatc aatttgcctg
      601 tatagaatta aatttatatt tagataacaa tccaagggat aagaaagctc ttgattcata
      661 caacagatac tgcgataaat ttacacaagc tgtgtgtgat tatgaatcta aatatggagc
      721 attaactaat tttggatatg aatcaagtga atatccttgg tcatggatat ctgaaccttg
      781 gccatgggac aaatcatttt ataaataaaa ttattaaact ttaaaattag atttattatt
      841 aaatatatat taaggaggag taagataata tgtggattta tcaaaaaaca ctggaacatc
      901 cagttaacat aagacaagca gaccctagaa tggcaaaata tatcatgact cagttgggag
      961 gacctaatgg tgagttggca gctgcaacta gatatcttca acaaagatat actatgccaa
     1021 ctggaaaatc tcgtgcactt ttaactgata taggtacaga ggaaatggct catgttgaga
     1081 taatttcttc agtgttatat caattaatag gcaattgtac tccagaagag cttaaggctg
     1141 ctggacttgg tagtaattat gctaattttg gacatggtct tcagccagta gattctaatg
     1201 gagtaaactt tactacaagt tatattaatg tctttggcga ttcggtaact gatttacatg
     1261 aggatatggc tgctgaacaa aaagcattgg ctacgtacta tcaattaata aatttaactg
     1321 atgaccctga tttgaaagat atattgagat ttttgggtga gagggaagta gttcactatc
     1381 aaagatttgg tgaagcatta atggatgttt atgagtttac agagtgcaag catcagtttt
     1441 aatgaagaat taaaagtgaa tggagagtaa gcatcatttt taatgaagaa ttaaaagtga
     1501 aaagagaata agcataagtt ctaatgaagt attaaaaatg aaaagagaat aagcatcatt
     1561 tttaatgaag tatcaaaaat aaaaagagtg taaatattag ctctgataaa ctatcaaaat
     1621 aagaggtaaa agttttagtt gatttctttt atctcttatt ttttattgat taaacagatt
     1681 actttgctat ttaaatatat tattttagca aaaaaagtcc aatcaaaagt tcaaagtatt
     1741 accatagttc aaagcattgc cgttgttagt aagtttttta caatattttt tatttactct
     1801 ttggataatc aaaagaaata aaaaatcata tctctttttt tatatttctg tacacttttg
     1861 gagaatatcc cgtttttctt taaaacattt actaaaatag attatttgat gaaaaacaac
     1921 ttttatagta atgtcactta ttagctcatt tgtgcctttt agaaattgag ttgcttttaa
     1981 taaacaaaac tcagtaaaat atttataagg tatagtattt aaacttagtt taaatcatct
     2041 ggagcactct gatttattga tattaccact gcttgatagt actccaaaat taagttttca
     2101 caatagtacc cttcaatata atgtaaaaca atagtatttt tcaatataac gtaaaatttt
     2161 ttgcatatgg atattaatta tatttttgtg ttttattggt ggcaaaacaa tattttttat
     2221 cataataagc caaaggaaag aaacagaaac taatgattca tacatataca gttcattttt
     2281 acttttttct aaccttatta atcgttgtag aatagaaaat actttattgc gccaattata
     2341 tttatgtgta aaatgataaa tagcaaattg ttcgttttct gttattctat ctactaatga
     2401 ttttactgga tttctaacat aaaaatactt ttctttttta cagcctattc ttacttttcc
     2461 catttgaaag tattatgata ttacttgatg ctatatcgat atttttttgg cctatttagt
     2521 gataaaatta taagatacac ttctataaaa gttaatacag taggaagctt aaatgtatga
     2581 attttatatt catcaaaggg aaagggaaaa aaggatgaac agatgcaggt aaatataaaa
     2641 ctaatatgat atgaggatag atattataac gaaagtgtag ttagaaggaa attatattag
     2701 tatgttttta cataatggta gatagaaaag aagtgtctaa tgatttgagt gaagttttca
     2761 gaatataagg aggaattgta tgcaatatat tagctattac catagtccaa ttggaaatat
     2821 tcttttagca tcagataata ttgggttaac tggtctttgg tttgagaatc aaaagtatta
     2881 tgcttataat cttgaccaag aacatcaaga aaaagaattg ccaattttcg agcagacaaa
     2941 gaaatggtta gatatttatt tttcgggaaa agaacccaat tttacaccat cactccatat
     3001 ggtaggtact ccttttcaga ctacagtgtg gaaaatttta caacaaatac catatggtaa
     3061 aacaatcact tatggagaaa ttgcatataa agttgctcaa caaaaaggaa tatctaaaat
     3121 gtcagcacaa gcaatcggtg gtgcagtagg gcataatgga atctctatta ttattccatg
     3181 tcatcgtgtt gtaggaacaa atgggagttt gactgggtat gctggtggaa ttgatagaaa
     3241 aataaaattg ttatctttag aaaaagtatc tgtgcaacat tattttatcc caaagaaagg
     3301 gagtgcatta taaagccatt aaatttagaa attcttaatc atttatttga tatacaaaca
     3361 tctaattttg aaagcaataa cttgcaattt taaaaaatga ttatttaaac tatatgttat
     3421 acaaccttat tcctagtaaa cattatacaa ctttagaggt agtttttaga gcttgtattt
     3481 tggaagttta ttttcttttt tcgtgtgagt agttatatgt ttagatgcag taatttatat
     3541 gctacaaaca ggaaaacaag gagcagacaa aaaatactta gataatatgt tttttgtgaa
     3601 aggttatgtt gaagaacttc tatttttaga caacagttat gatattgtat tttcatgtat
     3661 tgtatttcat cattgtacaa atgttcaaaa tatattttgt atattttaga aatggtaaga
     3721 gtgttaaatg taaggggaaa atttataatg tttgatactt aagctactca ttaagtatta
     3781 agaaccatac aagataaggt tgaaatttat atgcttcgtt ttatgtaaaa aatgtaagtg
     3841 agttagaaat aaaagaatta tttataaaaa tagattaagt attggaaaat atgaaaaata
     3901 gatgttgaac aaaacttagt cgattggtta aatttaacca aacacttaaa gtatcaaaag
     3961 aaaaaattat tactcttaag caagaagata taaatggaaa aatgaaaaca agattttatc
     4021 cttttataag tgataagggg atgtgtttta atcaaagatg gacttttatt ttaggctgaa
     4081 aaattatata actatattat agaatataag ggagatacac aactttttag tgttcaatct
     4141 cccttatttt ttattattat atcaagactc atttagtcgt gttaatttta ggataaaatg
     4201 agtatttttt aataaattat ttataagtaa agtgtgttgt cggataatca gtatttctgt
     4261 ataaaatttg gtataacttt aaagaagtat gtgaaatttt caggtatttt ttactatggt
     4321 atattattta actgatttta atttagcaat atcattcgaa ttcttagatg tgactatctc
     4381 tatattattt aaatcatttt taatatctga tgtatctttt actaatgaag aggtatgttt
     4441 aatcactgta tctaatgtat ttctgttaat ttttacatct gacttaattt ctgtaacatc
     4501 actctgaata gtatcaagtc tactactagt ttcaatttgt ccagttttta agtctttaat
     4561 atcaggttcc atattgtcta acctactatt gacttcttta atttcaactt gcatatcagt
     4621 aatactgtct tgcatactat taatagtgcc atccatacta ctgatactgc cttgcatatc
     4681 gctaatagtg ccattcatac cactgatact gccttgcata tcgctaatag tgccttgcat
     4741 gccactaata gtgtcttcga taccattaat attgctttgc atatcactaa tagtgccttc
     4801 gataccactg atactacctt gcatattact aatattacct tgcatttcct gtaataactc
     4861 taatattttt tcatccatca ctatcacact cctgttttat tattataaca taatttaggt
     4921 gttatattcc acaaacgcta tgtcatattt aatttttaat catataatta tctgctattc
     4981 aaaacatggt taaatatatc tatattgaga acttttttat taatgaatct tatgtattgt
     5041 ctaagtttct aagatactgt aataagtgtt tttgaggcat gtgtggttat atttgtattt
     5101 aaaactgctg gtaatatcat atacttttaa atatacctta agttatttat attgattact
     5161 tgtgtttgaa tattttgatt agtagcttcc tttttatctc tttttattct tagttttaaa
     5221 ccttcatatt tccttgaaaa tttattggca atgaaagtgc atgaattttt tagctatttt
     5281 ttatgttaca atatgaatat ataacaattc gacaaattgg atttttgaaa ggagtaaaat
     5341 gttatgaaaa tgccaaaaga gagtattgat acgattatgt ttgctccttg tggaatgaat
     5401 tgtatggttt gctataagca ttgttatcac aaaaagctat gtgctggttg tttgaacggc
     5461 gatacaggaa agccagaaca ctgccgtaag tgtaaaataa aggattgtgt ctgtcaaaaa
     5521 gggttgtttt attgttttga atgtttcaat tttccttgta aactcataaa aaatcttgaa
     5581 aaaagttata acaagagata tcaagcaagc cttatggaaa atagtaaatt tgttcagcag
     5641 tacggtttag agaagtttat gcaaaagcag aaggagagat atacttgttc taaatgtgaa
     5701 ggaattatat ctattcatga tagggaatgt agcgagtgtc aagaaaaagt gaaatagcta
     5761 aatcactatt tatcaagata gaaaaaattg taatatatat aaaggtggat tatattgagg
     5821 tggtatatag tgataggttt aaaatgtggt atggttaaat tattggaaca tcagatgata
     5881 tgggataaaa gtgcaaaaga tgtcattata ttattgaaaa gtatatggga taagactgcc
     5941 attgatattc aacacattgg aagcacttct attcccagta ttagcgcaaa gcctattatt
     6001 gatattgttg taggtgttgc ttctttagag gaagctaaat tatacctcga aaggttagag
     6061 caatgtggaa tcgtttttcg aggtcaggat gtaccaaaac aattactttt tgctatggga
     6121 gattttgaga aaaatactcg tactcaccat attcatgttg ttgaatggaa tagtgttgca
     6181 tggaataatt atatcaattt tcgagattat ctaaatactt ttacagacaa agcaaaactc
     6241 tatgatagtc ataaacaaaa attagcatta cagtttgagg acaatcgtaa aatctataca
     6301 cagagaaagc aagaattaat aaatattttg ctggaagaag cacgactgtg gagaataaat
     6361 acctaaaaga ataaattcac atttattgag aaattgagca attattttct tatatttagt
     6421 aagtaaaaat ctatttttgt tgattaaaaa atatctgtac acactcatga ctttagtcat
     6481 tggagattca gattgtgtag aattattgga tgaaggagga acagtatgaa aaaggataca
     6541 tctcatatta tcaaacgggc agagcacttt caggtaattt tgattggtga aggacttatg
     6601 gttggggctg tggctggttt gattgtgctt ttatacagga tacttttagg gcaggctggt
     6661 acttggctta gccaaatttt aaaatttgta gaaggttcta caataaaaat aatagcttgg
     6721 tttgcagtac ttgcagtact tgcgtggatt gtatcaaagt tggtggactg ggagccaatg
     6781 atttctggaa gtggtatccc tcagcttgag ggggaaatga cagggaaaat aaaacaagta
     6841 tggtggcgag tgttgccaac aaaatttatt ggaggattct tatcactcat gggtggtctt
     6901 gctttgggaa gggaaggtcc ttccattcag cttggtgcta tggcaggtaa aggtatttct
     6961 agagctcttg accgagggaa gacagaggaa aaattcctgt taacctgtgg ggcaagtgct
     7021 ggattatcag cagcctttca tgcaccactg gcgggtgtta tgttttcact tgaagaagtc
     7081 cataaaaact tttcagtatc tgttttaatt tctgtgatga cagcatcttt gacagcagac
     7141 tatatttcag ccaatttcct aggaatggag tctgtattcc agtttgatta aaaaatagct
     7201 acgcacactt gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac
     7261 ctatatgtag agataggata gaacctatcc aatactactt gaattgctgg aaacccctaa
     7321 agctaaccaa actaaaacat aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag
     7381 aaaaaattgg ttagatggta taaggttaaa tcctaagtac tttaaaatgg gcaatcagca
     7441 ggtaa
//
LOCUS       DAFKMX010000180         7353 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFKMX010000180 DAFKMX010000000
VERSION     DAFKMX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.182"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..795)
                     /locus_tag="LZB08_003136"
     CDS             complement(<1..795)
                     /locus_tag="LZB08_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7316603.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLK"
     gene            1266..1916
                     /locus_tag="LZB08_003137"
     CDS             1266..1916
                     /locus_tag="LZB08_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBM7316604.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(2056..3432)
                     /locus_tag="LZB08_003138"
     CDS             complement(2056..3432)
                     /locus_tag="LZB08_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7316605.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(3448..5361)
                     /locus_tag="LZB08_003139"
     CDS             complement(3448..5361)
                     /locus_tag="LZB08_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBM7316606.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            5497..6129
                     /locus_tag="LZB08_003140"
     CDS             5497..6129
                     /locus_tag="LZB08_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBM7316607.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            6268..6807
                     /locus_tag="LZB08_003141"
     CDS             6268..6807
                     /locus_tag="LZB08_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBM7316608.1"
                     /translation="MVFVEELWKKAIKFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(6983..7153)
                     /locus_tag="LZB08_003142"
     CDS             complement(6983..7153)
                     /locus_tag="LZB08_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7316609.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2708 a         1010 c          951 g         2684 t
ORIGIN      
        1 ttttaaacta tctacaaata tatcctcaat gtattttcca tatatatcct catttatgtc
       61 taaaatatct tttactttac tattatatgt atttatttca ccatcttttg aagtaaataa
      121 tataccatct tttgataaat ttaatattgt atctaattct tctattttta aaaataattc
      181 cttatattta tcttttatac cagtatctaa acttattatt tcttctgagt atttaactag
      241 attagattgt atttcctttg aatcaatttg taataaattt attatttcta taaatgtaga
      301 aatatctata tatctgtgac ctaaatcaaa tatatctgag ataaagctag gtactttttc
      361 tggaactcct ggagttatag ctatttttat atttttatag ttattatcat tcatatatgg
      421 aattggtcta atattagtca ctccaatttt ataaaataaa cttatagttt ctagagtagt
      481 ttcatcagaa tcatttacga ttagaacgtt tgtaccctgt ggtaaagata gtagattata
      541 aattttatct tccctaaaag ttcttcttac cacaatcact tttttcttat ctagtatatt
      601 atttataatt ttgtctaatc tttctactga cattactaat atcacatcat catttataat
      661 atcattatca tttatttcat ttatgaagta tctattaata tttatagaat ctccaaaaac
      721 taatttcaaa ttattttcta aaaagtctat taaatctttt ctactatcat ttaccagagc
      781 tactgatttt ttcatttatt tctccttata taataaatag tttccaacaa aatagttgta
      841 taaagcaata taattttgat aatttaaatt tatcaaataa aaaagaaagt gtctataatg
      901 actttataaa agttgcatta agacactttc tttcttataa ttaaaaatct tgtcaccaaa
      961 tccccctaat tgttgcttaa aacttaaact gtatgtatat ttttcatttc tattcgatta
     1021 aattataata tacacccaaa aaaatttatt ttaataaatt ctattgataa tataattttg
     1081 taaatataga attatattaa aataaattat atatgaaaat atatatgtat aagtttattc
     1141 tgcgtgagta gaatgaattt gtcaagttta atttagttgt aattgtatct tttattaaaa
     1201 ataatataaa ttaaaacata taaataaaat attttataca atatattgta aaggaggtaa
     1261 ctttgatgaa agcagaaaaa acaagtatat atccaattat taaaaataat ttaaacaaaa
     1321 tatcaattgt tgaatataaa aagggtcaaa gatttgtagc atcagataaa gacttacaag
     1381 aagtgttctt tataatagag ggagttgcat tagtggagtg tactactaga ggaggaaata
     1441 aatttcttgt tgacattgta cctgaaaatg aatttatagg caaaatcagt tacatatatg
     1501 aacataattt gaaatgtgat atatttgcaa agactaatat aaaattattt cgatttgaaa
     1561 aaaatgtatt tgaagagttt tatctcaaac cagattttat tgcactattt cacagaaaat
     1621 gtacaagaag aatatatgaa ttatataaaa ccagaatggt aagagaacta ttttcttgca
     1681 cagaagttat tgcctattgt atatcaaata aacaagaaaa taatgtctgc aatataaaag
     1741 ctattcgtaa tttcagtgaa atctattcaa ttagtagaaa aagtagatac cattctctgc
     1801 taaagttaac tgaaaaggag atagtaaaaa aaatgggtaa tacatatgaa attttaaact
     1861 atgatgaatt acataaattg gcatttgaag taaaagaatt tttggaagat gagtaaaaat
     1921 tgttacttaa tcaaaataaa gtataataat ttctgtaaaa aatagaaata gtctcactga
     1981 aaatcaaaaa tactttagtt attttcaatg agactgttta gaaatattgt atacttataa
     2041 tgtatagtat aattattaat caatttcctt tactatatta atagcccatt ttttagataa
     2101 tgctctagga acaccaaata taaacttaac cacttcttca ctataagtga gcaaaacgag
     2161 tacaaatata ggtgcacctt taaatgcagc tataaaagtt agaggaatag aagctaacca
     2221 catacatcct aattctaaaa atagagcata tttagtatct cctccacttc ttaaaatacc
     2281 aattataacc aatgtattaa aagatttaag agccattagt attcccatta taaagaatat
     2341 cttagttata tcagaagcta agctatcaga aacactaaac atttttaata ataatggtat
     2401 atttaaaatt aaaataattc caagtattaa accagctgaa aatacaagta tagaaaactt
     2461 tttagcatat tcaatggctc ttttaatatg gtcagctcca agttcatttc caagcataat
     2521 agatgcacca gatgcaatac atacagctgt cattatgaag aaattacctg tactggttgc
     2581 tatctggcta gctgctatag cagaagtacc agctcttgca tatgcaacag aatacaggac
     2641 agttccaatt gcccatacag agtcatttac aaatatagga aagctttttg atgaaaaaga
     2701 ttttataaaa attttatcaa tagcttttag attttttaga ccaaacttta atatatagtc
     2761 ttttttataa aggtaaacat aagtaatcat taaaattaat tcacatattc tagcaataac
     2821 agtagctaga gcagcacctt ttacacccaa tgcaggaaga ccgaagttac caaatataaa
     2881 tccataattt aaaataacat ttgttacaag tgcaaatgca ctacaaatca tacctaactt
     2941 aggatttcta acacctcttg aaccagtact aaatacagta cttacagcaa ttaatggata
     3001 actaaatact gcaatactaa agtaatctat acaaagtttt accacctcag aatcatacga
     3061 aaatatgtgt ataattggct ttggtgttag taaagcagga ataacaaata caaaacttaa
     3121 aacaattgcc aatacacaag taagacctgt gacttttctt atattactca catccttctt
     3181 tccataaaac tgtgaaataa aaactccagc acctcctgtg ataccagata aggacatatt
     3241 aaaaagaaag aaaaattggt ttgcaactcc aactgaagct acagatgttt cccccaaact
     3301 acttatcata attgtatcta ttacatttac agaagtagaa attaagttct gtatgataat
     3361 aggtatacat aaagatagaa gtattttata aaatctttta ttttcgtctt ttaaagaaaa
     3421 tagttcttgc aaataatcct ctccttttta cataaattct tcccactcat catatagaga
     3481 ggcaaggtta ttttctaaat taattttttc ttggctaact tctttagctt tatctggatt
     3541 agagtatact tcttcttgac ataaaagtat atctaatttt tcaatttcaa tctctaactt
     3601 ttcaatttct ttttctatat tttgtctttt tattctattt ttcttttcta tttctctttg
     3661 ttctctctct tttctttttt catcttttat ttgagttcta gttttttctt ctttttcttc
     3721 tataatactc atctcttgga tttgcctttt tttatctata taataatcat aatttcccag
     3781 atattcagta attccatttt catctaaaac taaaatttta tcaacaacag tatttagaaa
     3841 gtatctatca tgtgatattg taaatatagt cccatcataa ttagttaatg cctcttcaag
     3901 gacttcctta gaatcaatat ctaggtgatt agtaggctca tctaataaca ataagtttgc
     3961 atttgatagt ataagtttaa gtatggcaac tctagctctt tctccaccac tcaatgtaga
     4021 aatcttctta aaaacttctt catctacaaa taaaaatgcc cctaacatat ttctcaaagt
     4081 agtttgagta agatgagtat tattttccca aatctcatca attattgtgt tatctaaatt
     4141 aagagttttt tgttcctgat ggaagtagga aacatgtaca ttagttccaa atttaatatt
     4201 gccactaaga ggttgtagtt cattagttat tattttaaat aatgtagatt tgccaacacc
     4261 atttgcacct ataagtgcaa ctttttcccc tctatatata tctaaatcca agtctttaaa
     4321 aagtattctt tctccataac ccatagatac atctctaatt tgaagaacat cattaccact
     4381 ctggacagaa ggcgtaaatt gtatacgagc ttttttccta taagcttctg gtttatctaa
     4441 aacgtctacc ttgtctaatg ctttttcttt acttcttgca cgctttaggt gtttttctct
     4501 accataagct tttagtcttt caattgattc ttcttgcttt tttatttctt tttgttggtc
     4561 ttcaaatttc ttaagctcta gttctttttc tattttagat aactcaacaa atttagagta
     4621 gttaccatta tatactttaa gttttttgtt gtgaacttca aaaattctat ttacggattg
     4681 gtctaagaaa tatctatcat gagatattaa catgacagtc cctttatatt gttttaaaaa
     4741 tacttctaac cattctatcg cttcagagtc taagtggtta gtaggttcat caagtaacaa
     4801 tagagttggt ttcttaagaa gtaattttcc taaaagaact ctagtttttt caccaccaga
     4861 aagtatgctt attggcttat ccatatcatt gtcactaaat cctaatccct ttagaactcc
     4921 tttggcttca gatttatatc catatccatt taaatctgaa aataattcta gtttatgtga
     4981 atattcatcc atgatttttt ctataattgg agagtttgtt tttgtacttt cttcagatat
     5041 tttaacctcc aattctctta aataggattc catttctatc aaatttttaa aaacttctag
     5101 tacttcttcc aaaatagtat tatccgaata aaaattggta ttttgttcta aatatccgat
     5161 ttcacaatct ttagaagtgt aaatatctcc actatcatat ccataaattc ctgaaattat
     5221 tttaaataaa gtagtttttc cagtaccatt aacaccgatt ataccaattt tatcgccttc
     5281 atttactgta aaactaatat tttctaatat agaatctata ccaaaacttt tatttaagtt
     5341 attacatgac aaaacaatca taaatttcta cctccttgaa ctatctaaaa ttataatgtt
     5401 ttgatataat ataatcaagg ttatatgtat tagttttgaa aaagaaatat tttattgtga
     5461 atcaaacaaa aaagaggaca cttttaggag gtgaagatgt tgggaaataa aaatatatca
     5521 atggcagtta taagaaggct cccaaaatat catagatatc ttggagactt attagatagg
     5581 gatatacaaa gaatatcttc taaagaattg agtgatataa tagggtttac cgcttctcaa
     5641 ataagacaag atttaaacaa ctttggtgga tttggacaac aaggatatgg ttataatgta
     5701 gaagctcttc atactgagat aggtaaaatt cttgggttgg atcgaccata caacgcagtt
     5761 cttgtaggag caggtaactt aggacaagct atagccaatt atgcaggatt tagaaaagct
     5821 ggattcgaga taaaagcttt atttgatgca aatcctagaa tgataggttt aaagataaga
     5881 gagtttgaag tattagattc agatacttta gaagacttta taaaaaacaa taatatagat
     5941 attgctgtat tatgtatacc taaaaatgga gcacaagaag ttattaatag agttgtaaaa
     6001 gctggaatca aaggtgtatg gaattttgca cctttagatt tagaagttcc gaaaggtgtt
     6061 atagttgaaa atgtaaactt aacagaaagt ttatttacct tatcgtattt aatgaaagaa
     6121 ggaaagtaga caacttaaac atattaataa tgagcagttg tgaagagtat tgagaaagtc
     6181 agaagattta atattaaatc ctctggcttt ttgaaatatt aggataaaaa tttaaataat
     6241 gtatggaaat aaacaatatt aaggagagtg gtttttgtgg aggaattatg gaaaaaggca
     6301 attaaatttc atggtcatga atgtccagga ttagctatag gatttaaagc agcacttgca
     6361 gcaagagaat atttaggatg taaacagtca ggagatgagg aaattgtatg tatttctgaa
     6421 aatgatgctt gtggaataga tggtatacaa gtcgtgctaa gttgcacaat gggtaaagga
     6481 aatttactac ttaggatgac tggtaaatca gtatatcatt tctttaatag ggaaaatgga
     6541 aaatctataa gaatatatgt aaagtcaaag ccatttcaaa tggatagaga aggatacaaa
     6601 aagtacttat tagaaagtga ttttgaagaa atatttgaga ttacagatac taagttaaga
     6661 cttcctgaaa aagcaagaat atttaaatct cttaaatgtg aagtgtgtgg tgaatttgca
     6721 tctgagcaat atataaggat tgaagaagga aagaaagtat gtcttgattg ctttaataaa
     6781 tatgaaagat tttatgagga gtattaaaat tataaattag attaaagtta aaagtattag
     6841 acatgtgcta acagtatttt aagatgaatt aggtagcaac ataataaagt atataaataa
     6901 tattaattga atcatacata attaaaaaga ggctgattag tatttataaa ataaaatcag
     6961 tctctttatg aataacgaat tattattctg gttgaggtgc atctacagga caagctcctg
     7021 cacaagaacc acagtctata caagaaccag catctataac atatgcgtcg tctccagctg
     7081 atatacagct aacagggcat tcagcctcac aagcaccaca gcttatacaa gcgtcagtaa
     7141 ttttatatgc cattttgaaa tcctcccttt tatatgtatt gtaattcaat aacattataa
     7201 caaatattat tgaatataac aattaaatta attctacaac aaaaacttta ttatgatatt
     7261 cttgttttaa agttttacaa taaagtttaa tctgcttata tttttattta ttttaaagaa
     7321 gtttatttta aaaataaaat taaatttaaa aag
//
LOCUS       DAFKMX010000181         7301 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFKMX010000181 DAFKMX010000000
VERSION     DAFKMX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.183"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            195..845
                     /locus_tag="LZB08_003143"
     CDS             195..845
                     /locus_tag="LZB08_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316610.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1161..3227
                     /locus_tag="LZB08_003144"
     CDS             1161..3227
                     /locus_tag="LZB08_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBM7316611.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3244..5199
                     /locus_tag="LZB08_003145"
     CDS             3244..5199
                     /locus_tag="LZB08_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBM7316612.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIISHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5215..6405
                     /locus_tag="LZB08_003146"
     CDS             5215..6405
                     /locus_tag="LZB08_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBM7316613.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         2702 a          858 c         1366 g         2375 t
ORIGIN      
        1 atagtacaat aacttgattt gcaatacaat gaattaatct atagtataag agcatgattt
       61 acagtacaat aatttaaatt gtagtacaaa agtactatca aataagaatt tctactttaa
      121 aaatattact aaaagtaatt aaaatcaaat aattcaatat aatatggcaa aagaatagaa
      181 ataaggtgag agtagtgaaa gtaaagtcaa atttagatga aattttatat cataagatta
      241 ttgaaagtct tattcgtggt gaatactctg taggtcaaaa aatattacta aatgaccttt
      301 gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc agtaaaaatg ctaaataaag
      361 atggagtgct tactattatg acaaatggta aagtgtacgt accagagtat gaatatgata
      421 tggtaaaaca agtttgtgaa acgagaacac tgattgaaac atatgcttta gaaaaaatga
      481 tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt aataaaaaga tactctgata
      541 aatgcgagga gtactatcgt caagaaaaat ctgtagaact tgcactagca gatttggaat
      601 tacataaagc cattgtagaa ggggcaaata atgagatttt aaatgatgta tatgttggta
      661 tacaaggaag atttatagta gtgaactatt taattcgacc attaaaaaat cgtaattatg
      721 aaggaacagt acaagaccat tttgaaatat taaaattcat tgaagacaga gatacaaaaa
      781 aagcagtaga taaactaaga aatcatattc aaggaactat taaaagattc tgtgaagatg
      841 aataataagt tttgaagtta ctatatatac ttatctatta agttgctaaa tgtagtctaa
      901 gtataatcta attatagttt aaacataatc taaatataat ctaataaata taatctaaat
      961 atagtgtaaa taagtctaag tatgaattaa atatagatta agtatggact aagtatagat
     1021 taagtatgaa ttaagcatgg attaaatata ctttaaatta caaatattac aaggaagcca
     1081 gaaggcttcc taaaaaaata acctttataa aattttttat tttaaattta aaatttttta
     1141 ttttaaattt aaaataaaaa atgaaagagg tggtagggag tttgaaaaaa attactaaag
     1201 aaaggataga aaatactatg aaaaagctat ttacgagtga aacatttttg gaatatcaat
     1261 ttataagtga ttgccaaata tcaccagatg gaggatatac agcatttatt gtaaaaaaag
     1321 ctgatatcaa agagaatggt tacacaagcc aagtttatat attaaacaat aaaactggcg
     1381 aattaaaaca aataacgtct ataaactctg ttggagcata tgcctgggaa gatgaaaata
     1441 ctatcttgtt ccctgctttg cgaaatgaaa aagtaaaaga agctgtcaaa aacggtaagc
     1501 aatgcatgag ttactatgct ttatctttaa atggtggaga agcagaagaa ctttttagac
     1561 tccctatcaa aggtggtaaa ttgaatccta taggaaaagg tttgtatgca gttattgatt
     1621 cttatgacaa tgaccgacct attgtagagg gattgccaga agaagaacaa cagaaaaaaa
     1681 taaatgctta taacaaaaga cattatgaac atttcaaaga aattccttat gctgtcaatg
     1741 gggagggata tattagcaga aaaagaaaac gcctatatgt atacgacagt aatataaatg
     1801 agttgaaggc aattacagca cctatgttta atgttgtagg tatgaaaata agtgatggaa
     1861 aaatcttata tatagggcaa gagtttaaag atgtaaaagg tttgaaaaat ggggtctatg
     1921 tatttgatac aaacacaaac acaaatgtct gtatattaga aaaagataag tatattataa
     1981 aaggttttga attatatcaa aatcaagtga tactaaacct aactgatgct ttatcttatg
     2041 gaaatggaga aaatggagat ttttatacta ttgatattga tacgaaagaa atgaaacttc
     2101 tttcagggca tcaacatcat tgtattggaa atacagtaac atctgatgtt aaaatgggtg
     2161 ctggtcagac aacaaaggta gatggagaat atatttatta cacttctaca gtagatatgg
     2221 actgcattat tgaaagaata catattccaa caggaaaaca ggaaaaagta acacagacag
     2281 gttcagttga ttttatagat gtaaaagatg gcaatattgt atgtgtgggt tgtttaggaa
     2341 atggattacc agaagtttat acagtagaga atggaacatt gtgtaagaaa acacatttaa
     2401 atgaccatat tttagaggaa tataaaattt ctgtgccaga atacatagag tcaaaaggaa
     2461 gttctaaatg ggaaatacaa ggatacgtat ataaaccagt agattatgag gtggggaaaa
     2521 aatatccagc tgtattggcg attcatggag gaccaagatt gacatatgga ccatatttta
     2581 tgcatgagat acaggtattt acaagtgcag gttactttgt attcttctgt aacccaaggg
     2641 ggtctgaagg tcgtggcaat gcttttgcag atattaggaa acagtttgga gatattgatt
     2701 atatagattt tatggaattt acagatactg tacttgaaaa atatacagac attgataaaa
     2761 caaaattggc tgttgaaggt ggaagttatg gtggatttat gacgaattgg ataattggtc
     2821 atacaaaccg ttttgctgtt gcttgtgctc agcgttctat tgcaaactgg agtggcatgg
     2881 agggtacaac agacattggt tattatttct gcaaaggaca aacaggtgct tctcatatgg
     2941 aaaaccacga gcttcaatgg aaacagtcac ctcttgctta tgctgataaa tgtgttacac
     3001 cgactttatt tttacatggt gaaaaagact atagatgcta tatgcaagaa gcattccaga
     3061 tgttttcagc attgaaaatt catgggtgtc ctacaaaact ttgtctattt gaaggagaaa
     3121 accatgaact gtctcgttct ggtagaccaa aacaaaagct acaaagatta gttgaaatgc
     3181 tagattggtt ttctgtgtac ataaaaaaag ataacatagg acagtagaaa ggagaatata
     3241 aaaatggaaa aaataaagat tgaaagttca gatatttttg aatatatatt ccctcatgat
     3301 atttcttgtt cacctgatgg aaagcatatt gcttatatta tatcaaatat caacgaggaa
     3361 aaagactgct atgaacatga cttgtatgtg atggacatca aaacagaaaa acaaatacat
     3421 atgactcaaa caaaagatgt aacatcattt agttggattt ctaatacaga actccttttt
     3481 acatctaaaa gaaataagcc aaaagcaggt acaacagatt tttacactat ttctatagaa
     3541 ggaggagaag caaaaaaagc attttctatt ccaaaagctt gtagcgtacc tgtatcactt
     3601 ggaaataaat tgtggttatt gacaacaaaa aatccaacgg attctaaaaa atcagaacca
     3661 gatagggcag tagagggtgt agattattgg acatttacag ataaaccttt tataagagat
     3721 ggagaaaatt tttctcaaag aagacgtgta acattagaat tatatcaaga aggtgaaaat
     3781 ataacaaaag ccattacacc aaaattttgt gaagtagcag gtgtagacgt ttcttctgat
     3841 aaaaacagaa ttttatatac tggtcagatt tatgaagatt gtgcaactcc tttttctgga
     3901 ctttgggagt atcatataga cagtggtgaa accaaagaac ttgtaccaca aggaaagtac
     3961 caaataagtt tggcaaaata tattggcaaa gataaagtaa tgttgcaagc ttcaacatta
     4021 gaccgttcta ttacacagaa tcatgatatt tttatacttg atttgagtac tagtgaaata
     4081 aatatgattg ctagtccaga tggtatgtat gctacattgc tggatgtaga tgcagtatat
     4141 ggtggtggac gtagtaataa agttatagga gacaaattta ttggtgcaag aatttgcaga
     4201 actatgacag aatttaatga gtttgataca aagacaggta atatacgtat catcacaaaa
     4261 gtagatgctt ttacatcttt tgatatttac gacaacacca tgtacacagt aatgttgaaa
     4321 gattatgagt tagcagaaat atatagcatt gatatgacta caggtacaat gaaaaaaatg
     4381 actgcttttt ctaaaccata tttagatact cataaggttt cattacctga aaaattgacg
     4441 tttgtagcaa aaaacaaaga agaagttgat ggttttgtta taccacctat agatgctaaa
     4501 gaaggcgaaa aatatcctgc tgtattgttc attcatggtg ggcctaaatg ggcatatggc
     4561 tatatgttta cacacttgaa gcagtgtgta acatcaaagg gaatgtatgt gatttattgt
     4621 aaccctcatg gtggtgacgg ttatggtgaa aaattcttag aaatggtaga aagatggggg
     4681 tatgttgact atgagcatct tatggaattt gtagatactt gtattgaaaa atatcctggg
     4741 attgatgcag accgcttagg ggttgcaggg ggaagttacg gtggttatat gacaaactgg
     4801 ataattagtc atacagaccg tttcaaagca gcagcttctc aaagaggtat tagtaacttg
     4861 attacagctt ctttaattat tgactttggg gatagaatta tgaagcagac ttgtggagat
     4921 aagacgcctt ggaatcatga ggaggttctt tggaatcatt cacctattaa atatgtgaaa
     4981 aatgttaaga caccaacatt gttcttacat agtgataggg attatagatg ttttatgggg
     5041 gatactttcc aaatgtttac agcattaaaa caacttggtg tagatacaga aatgtatttg
     5101 ttccacggag atacacatgg attgtctaga aatggaagac catctaatag aattgcacga
     5161 gcaaatgcta ttgtagattg gtttgaaaga tatttataat aagggggtta ttctatgagt
     5221 gatatgttat tatggtttag tgtcgtatta gttttatttg ctattgggga tttaattgca
     5281 agcaaaacaa aagcaaaagt atcagctgta tttgttacac tacttttatt tttgattcta
     5341 tttgtcacaa aagcaatacc agctgatatt attgaaaaag caggtatgac cgcagcagct
     5401 agttggagtg ttccaatgat tatgtttagt atgggaacaa tgttgaatgt taaacaattt
     5461 atagacgaat ggagaacagt attgacagca tggcttggaa ttgtagcagt tatagtctgt
     5521 gtctcattat gtattccttt gtttggaaaa tcaacagtat tgacaagcat accagttata
     5581 aatggagctt tacctgctac tactattatg acacaagcag cactagaaaa aggactgaca
     5641 cttgcagcag ctacagcaac agttgtattt gcaattcaga agtttattgg tacaccaata
     5701 gcttcaagag cagcgttaca agaggcaaac cgtcttttag tagaatatca tgaagcaaaa
     5761 tcaaaaggca ttgatttagc aaatgttgat actgataaga aagaagcaga gaatacaggc
     5821 acaaaagtaa aacaagcttt ctgtgaaaaa tacgataaat attattctac aaatgtttgt
     5881 atatttttta tagcattatt ctcttatcta gggtatgaac tttcagagat tattcatgta
     5941 aattattcta ttgtatgtct tgttgttggt gttattgtaa cacgtattgg tattgtgcca
     6001 aaagacattt tggaaaaagg aaaaatcaag ggatttatca acatggttgt gtttgcagct
     6061 gttattcctt ctttagcaaa agtttctttg acagatttaa ttagtctttt tgtaccaatt
     6121 gtgggtatgt ttgcagcttc tatcattggt atattcctta tgatgaaggt gttaccaggt
     6181 tggaaaatta tcggttctaa acctcttgct tttggagtag ggttttgtca gatgttggga
     6241 ttcccaacta catatttaat ttcaaatgaa gtgtgtaatg ctgttggaga aacggaagaa
     6301 gaaagagcat atttgatgtc taagattatg cctaagctgg tagttggtgg tatggcttgt
     6361 atgatttcta ttgtagtagc tggtcttatg gtaccaatgt tgtagatagg tttcaaagtt
     6421 aaaaaagtgg tattaattta taggagaagc taaaaagtag tattaattta taaaataatt
     6481 ttatattagt taatttgtta taagaatatt ttctttttat aattataatt ttgattttat
     6541 aagatagcag attttttatt tgctatgcta atagttataa tttgttttta tttgctaaaa
     6601 tgcaacttta atttgattta tgtataatat agggtattta ataatatttt tgaagaattt
     6661 ttataaaaaa ttaaaaagag gagaaaaaag ggagtgccac aaagtgaact ttttagttct
     6721 aaggcactct tttttgtacg aaatcgaata tattttcaac gttttaaaag tgagaaagag
     6781 acttatctct agtttgagat agccaatttt aattattaaa attatttttt gttttattta
     6841 tcaaagagta ttcgtgtttt agaagttagt attttttatt ttatatatac aaggttttcc
     6901 gtttctaatg ttttcttttt taaaataatt agggtattca attatagtaa aaggtggatg
     6961 gtattttgga gtgatattat ttaaagcaca gtattgaatg ttgaaatcct cacattttag
     7021 agtattatct tttgtaatca aaaaaatctt ctatacttat agcaaggtta cttctgcaaa
     7081 tattccatac ataataatat cccaatttaa atttttttaa tatagagttt acctctagtc
     7141 tacactcttg aataactctt tcctctatcc aaattcttat aaattcatcc catttattac
     7201 tatttattta ttattatcta aattttttag tatgtttgta attagaaatg gatttattcc
     7261 tttatgtttt ttataaatag tttataactt aatttatcat t
//
LOCUS       DAFKMX010000182         7253 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFKMX010000182 DAFKMX010000000
VERSION     DAFKMX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.185"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            411..788
                     /locus_tag="LZB08_003147"
     CDS             411..788
                     /locus_tag="LZB08_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7316614.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            781..1929
                     /locus_tag="LZB08_003148"
     CDS             781..1929
                     /locus_tag="LZB08_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBM7316615.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVRNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNTNEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1946..2548
                     /locus_tag="LZB08_003149"
     CDS             1946..2548
                     /locus_tag="LZB08_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBM7316616.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2641..3663)
                     /locus_tag="LZB08_003150"
     CDS             complement(2641..3663)
                     /locus_tag="LZB08_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBM7316617.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKIRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3992..4174)
                     /locus_tag="LZB08_003151"
     CDS             complement(3992..4174)
                     /locus_tag="LZB08_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316618.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4487..4906
                     /locus_tag="LZB08_003152"
     CDS             4487..4906
                     /locus_tag="LZB08_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBM7316619.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            5037..6878
                     /locus_tag="LZB08_003153"
     CDS             5037..6878
                     /locus_tag="LZB08_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7316620.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6905..>7253
                     /locus_tag="LZB08_003154"
     CDS             6905..>7253
                     /locus_tag="LZB08_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7316621.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNN"
BASE COUNT         2974 a          775 c         1019 g         2485 t
ORIGIN      
        1 tataaaagtc ttattcaata aaatttataa ttaatttaga tatatttgta aataatcagt
       61 ataaaataga tgttttaata gcataaaggc ataattagaa ggttgttaaa atttattaga
      121 tattactcta gtttatttta aaaaaaagta ttataatatt ccttgtgtga aaatatagga
      181 atttttatta aatagtttta atgtgtaaat aacattctac actattttaa ttaaatatgt
      241 aaaaagtaaa atatttaaat agatatattt taaaatctaa cttttaatat tgtaaaaaaa
      301 ttagaaattt aacaaaaagt acaaaaattc acattttttt cataaagtgt ttgactaaaa
      361 aattcacatg ttgtagaatt aaggtaacag gagttttgag ggaggtgtaa ataatgataa
      421 gaaaaatacc acaagcagaa ctaaaagtaa tgaaattcat ttggaaagta gatgttacag
      481 tgacatcaaa agatgttatt gaagctatgg aacaaaaata tggctggaaa caaaccacca
      541 cactgacact tttatctagg ctggtaaaaa gaggattctt agatgctcaa aaaatagaca
      601 gatatacaca ttacacaata agagtaaaac acaaagatta tttaagtttt gagacaaagg
      661 atttcctaag taatatacat gatgactctt taagaagctt aatttcagca ttacatgatg
      721 atgaaaatat aggtaaagat aagttagatt ttctagaaag ttattttgat aacttaaagg
      781 atgaataatt atatgctata tgtttttgaa aatcttataa agacaactat tgtatgtagt
      841 cttggtattt gtctattatt atttttgaaa agatatttat ttaagaagtt tagtaagaga
      901 ttcaattatt atatttggtt gataatagtc tttagaatgt tactttttct atttaattat
      961 acaatagtat atgaagtaaa agaacccaaa gaaaatgcag taagaaataa tattacacaa
     1021 attgatattt ctacagataa taatcttatg ctgtatgtag cttatttatg gttatttgta
     1081 actatagtca ttgcagttta tacatttata aagtatacaa gatttaaaaa tctagttgtt
     1141 gatgtatctt atgatattga agataatgat ataaactgta tctacaaaaa cttgttaaaa
     1201 gaattaaata taaaaaagaa aattgaatta agaggttctg atgaattgat aagtccagca
     1261 ggcatgggat tatttaaatc ttacatattc ttaccagact acccttatag taaagatgaa
     1321 ctaacttgga ttttgaaaca tgaattaatg cattttaaaa ataaagatat tttaataaag
     1381 tttttagttt tgtctgttag aataatatat tggtttaatc cacttgtata tgtaatgagc
     1441 aataaagtaa atctagattg tgaattatgt tgtgatgaaa gtgttttggt agaatgttct
     1501 ttaaaagata aaaaagaata tgcactagca cttataaagt caattaagtt tagtaaaaat
     1561 tataatagta agattttgac aaccgagttt gataagacaa atttggaaaa gagacttgag
     1621 agtattgtta aaaagaaagg caaaagtgga atactaattg ctgttttagt atttatgact
     1681 tctataacat cttttgttga agttgatgct caagttagaa ttgatgaggg taataatgga
     1741 atgaataaca atactaatga atttttattg aataagacga ctgataaaat tataaatatt
     1801 tattataaag atgcaccaaa tgaaattagg aaattttatg aagataaatg cgaattagaa
     1861 ggcaaaatac ctagagacta tgacataata gaaatggata tgaaagatta taatcaatta
     1921 ataaaataat tataaggagg attttatgtt aacaaaatta agaaaaatag tagctattat
     1981 gtgttgctta ttgcttactt ttacaagtgt ggcatttgct gacacaagta aagagaaagg
     2041 aggaagtaaa gagactgttc cagagaaaaa cctagattct tttactcttt ctgggtatat
     2101 atatacatat gaaaatgcgc cacaagaaat aagagatgaa cataaacaaa attgtgaaga
     2161 gataaatata gaccctaagc cagatgatga gatatttgtt ccagaatcag ctaatctaat
     2221 gaatgaggac aatagtaaag gtgtttatgc aagttatact gtttcttata atataggtgc
     2281 taaaactata acaattatga gtaatgagta tttgactata agtacaacaa aagttatacg
     2341 aaagggtaat agcggaaaag aagtaaaagc tgcacaaatc atgttaactt tacttggtta
     2401 taatgtaggt atagatagtt cttttggaag taagacatat aatgcagttg taagtttcca
     2461 aaaaaaatac ggtttgtctg cagatggaat aattggtcca gctacttggg ataaattggg
     2521 tagactaact gaccctactc tttcatagga ttaaaatcta aaaatatatt aaatagtgtc
     2581 tcaatatgat ttttcaattt aaatgagaca ctatttaaat ataaatgtta taaaactatt
     2641 ttacaataaa ttccatataa ctctaccatc tatatcctta acattttgac aaccagtcaa
     2701 aatcatagtc tcacataact cattcctaat cttctctata aaaaactcca caccatcaag
     2761 accaccgcca aaagaagcag taacaaaagg tcttcccatc aaaacagcat cagcacccaa
     2821 tccaagcatc ttaactacat caacaccagt cctaactcca ccatcaacaa gtattgttat
     2881 ctttcccttt acagacttag ctatatctgg aagtacctca caagtaccag gagtataatc
     2941 aagtactctt ccaccatgat ttgaaacaat aatggcacta gcaccagact ctacagccat
     3001 caaagcatca tctacagtca taattccttt tataataaaa ggtaatctag tggattcaac
     3061 caattctcta atctcatcaa ttgttttagg agaaaaagga ttttcttgaa attgattatt
     3121 aataagtcca caagcatcaa ggtctacacc aactgcaaaa gcacctgctt cttcagacaa
     3181 tctaatttta tctattatct ttgaattatt ccaaggcttt ataaatacaa ttccatttcc
     3241 cctattatcc ttcaatacat ctaaattatc cagtaaaaaa gtatctacat tagtatcgcc
     3301 aaccatagca taaattccag aattagaaca acctctaaca acaggctcta tgtattcttt
     3361 ttcggaaact ttaccaccca tgttcaatat agtacctgat acaggtgcag cgaatatagg
     3421 agaactcatt tttctaccaa ataattctat ggatgtatct ggttttgaaa cattatgtat
     3481 tactctcata tttatcttta ttttttctag actttttcta ttttcaatga aagacgaacc
     3541 acttccttta ccacccatac caggaacatc tccagcacaa gctaatccat tacaaatttt
     3601 acatacttta catgttccat taaagttctc tctagctgat tttagtaagt cattgtaatt
     3661 catacaaaat ctctcctttt tataatatta aaattgagta ttactatatt gaaaatatct
     3721 taattgtaaa tatagggatg ttattatgat gagctaaact agataataca tattagatat
     3781 tttttattgt tgtagatagt taaattatat catattagat agtcaaattt tgaaaaaaac
     3841 taacttattt aagtatacat tttgaaaaaa ttaaaaaagc tatatataga tttttaaaga
     3901 aatctatata tagcttttaa gtttatgtca atcttttttg ttaatgaata aataatatag
     3961 aatacaatat acaaaaaagt ttcttgaaat gctaagataa tccccattta caaagcaaca
     4021 tcattatact gtgtaaattt gaatcaacaa tgttttcacc atatttcatc ataaaatcct
     4081 ctaattgtgt gtcaataaat gaataatatc cacttatact atcaatgtat gcagtcatta
     4141 agtgtataca ttcatcactt ttatctttat acatatatat ctccccctat atacaatata
     4201 taaatttaga taccaaatcg tatcatatta aatagttaag tgtgtaacat ctatgcagtt
     4261 atatatttat atcattatat taataactaa agtgagtttt gacaatagat tttgggaaac
     4321 tggatgttca aagtgcaaac ttggatttta aaatcattaa ttaagatagc gcataggaat
     4381 atatgtatac ataacttaat tataaagatt ataaacaatt acttttatta actttattta
     4441 aatggtagaa taacttggag attaataatt ataaggagga tataatatga gtttatataa
     4501 gtttatatat gatgataagg aatatttatt aaaagaggag aactgtagtg ctttaataaa
     4561 tgatgaagaa aatccagttc aaggagtaag tatttcaaaa ataattgata tacttaatga
     4621 agcagaagaa gttgattttg atgtagaata ttatcaagaa gcatgtccat tatgtttaga
     4681 aggagtaaaa gaaaagaaaa aattctttcc ttttttagaa tatcactttt atattttcag
     4741 taaagatgga gaatatgtaa taagcaatat atctgttgat tataaaggac tatcttttaa
     4801 taaactatca agggcaaata aagttgataa tagttatatt gtaagtgtga taatttgcga
     4861 aaattgtcag gattatatag ttcagataga aaattgtata gtttagtgta aagtatgtct
     4921 taatttaaga gaaatttcta aattgagaca tcaacttttt tcaaaaatag tatggaaaat
     4981 tactaaattt atgagataat gtaagtcgga ggtgagttac aatatatagt aaatatttgg
     5041 aagagataga tgatacaatt ttagattcaa tgtttaaact aaccttgaat gaaagattat
     5101 tgactaataa cgattttatt gaaaataaaa ttgatgtagt aaagaattta attgattgct
     5161 ataaaactga acaaaatagt ttaatgttat taaaatcaaa ttttttgcta gctttattat
     5221 atggtattca agggtttagt gaaaaaatga aagagtatat tttgatttcc tgtaaatata
     5281 ttgataaatg cttacccaaa gattataaat ttttagcacg attttattca caaatagcta
     5341 ttttaagttt aaaaaatgaa gatgtaaata aggcaaattt atatatggat aaatttaatc
     5401 ttatttgtga tgaaaataat tttctaattg aagaaattat ttttaaatca caacttattt
     5461 acattaaggc aagtaagtgt atagaatctt ctgtaataat taaagaaata gaaacgttgt
     5521 atataaagat aaaagaaatt aacgatttta attgtaaaaa aatatatttt tttattattg
     5581 gtaaaatata ttttttgttt ttggatgatg ctctaattgc taaggtaaat tttttaaaag
     5641 caagaaaata tgcagattta ctaaaggata tgcaagtatc ttcactatgt aatataaaga
     5701 taggtgagtg tgaatgttcg tttgaaaatt atgaatgttc catggaatat ttcaatgaag
     5761 taataagcaa taaaaagtat agaaatgtaa atataattca aaaatataga gcatcaaaca
     5821 acataactaa aatattaata aagactagaa attatcctgt cgcaataaat aatttagcaa
     5881 aatctgaagt atatctagaa aaaattaaaa atcatagctt aaaagaagta gagaagtttg
     5941 atttatttat aagtttagcc atgtattatg caaaaagtaa tgaaaaatct tttgaacaat
     6001 caatatgcta tttagataga gctaaaaaca tattaaatat gataaaaaat atagaagaat
     6061 atataatata tgatttagaa ataatttatc accaggcaaa gatatattat atctttggat
     6121 attatgataa tgccttaaac acaaataaaa aactccttga aaaatctaaa aatgctaaaa
     6181 attataatta tgtgaagtta gcatacaaga gtatatattt gtgttttgaa aagattcaaa
     6241 attatgaaat gagtatgaaa tactttaaaa tgtattatga gttaaaaatg atgtatataa
     6301 aagctcgaaa tagaaattat atagattcct taaattatag acatgagtat atagaaaaag
     6361 aaataaataa catgagaaaa gtaaaacttg atttagataa aaaaaagtat gttgaccatt
     6421 tgacaagtgc atataataga acttttttga acgatttttt agaaaaacaa gaagttagtg
     6481 aatattgcac agcatttatg atagatgtag attactttaa agaatacaat gatacttatg
     6541 gacactacaa tggagatatt gctcttaaaa atataactat aataataaaa aaatatctaa
     6601 aaaaagacat gcttatgata aggtatggtg gagaagaatt tctgatatta tctgtatgta
     6661 aagattataa aaaaagtaaa atatttggaa gaaaattatg taggattgta aagaaatttt
     6721 taaacaataa tttgacagtt agtattggca ttgatacatg taaaaatagt agtatagata
     6781 taaaagaaat aattgaaaat gctgataaag ctttatataa agcaaaggaa agtggtagaa
     6841 atagatgttt gcattatcat gattttaaga atttttaact gttgaaatag gattggtgat
     6901 ttacttgaaa aggagtttaa tagaagattt aaagagaaat ataaacaaga tagaatacga
     6961 tatgttgact aaattctatc taaatttgac agatttaaac tacatcttgg attatcacaa
     7021 agacctttta aatacaaaat tagaagataa tagtagagtc tatactttat atttaattag
     7081 tcttataaac tatataaaaa aagatagaga agcatcatac aactatttga tagagtcatt
     7141 aaagtatatt aaaaaattgt cattaaaaga tgattcagat ataatatcta aaatttatat
     7201 ttatcttact ttatcagcta tttctataag aaaatatgat aaggtaaata att
//
LOCUS       DAFKMX010000183         7096 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFKMX010000183 DAFKMX010000000
VERSION     DAFKMX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.186"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            67..1191
                     /locus_tag="LZB08_003155"
     CDS             67..1191
                     /locus_tag="LZB08_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBM7316622.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1665..2489)
                     /locus_tag="LZB08_003156"
     CDS             complement(1665..2489)
                     /locus_tag="LZB08_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316623.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(2837..3892)
                     /gene="ispG"
                     /locus_tag="LZB08_003157"
                     /gene_synonym="gcpE"
     CDS             complement(2837..3892)
                     /gene="ispG"
                     /locus_tag="LZB08_003157"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBM7316624.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKRVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(4075..5079)
                     /gene="rseP"
                     /locus_tag="LZB08_003158"
     CDS             complement(4075..5079)
                     /gene="rseP"
                     /locus_tag="LZB08_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBM7316625.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(5092..6246)
                     /locus_tag="LZB08_003159"
     CDS             complement(5092..6246)
                     /locus_tag="LZB08_003159"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBM7316626.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(6410..6613)
                     /locus_tag="LZB08_003160"
     CDS             complement(6410..6613)
                     /locus_tag="LZB08_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316627.1"
                     /translation="MISYAPLWKLLIDKKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         2417 a         1214 c          723 g         2742 t
ORIGIN      
        1 attttacaat ctttcttcaa ttgatatata atatgtttat ataaactaat gtatgtgagg
       61 tgaaatatga gcaaaaaaac aaaatatttt tctttaattc tctttcctat tgctttgctt
      121 ttaaatttta tagccagtaa agttccaaat attgttgaaa aatattactc ccaattcata
      181 gataaaataa ttgtacaaat attgagtaaa gtttctggaa ttttcccatt ttctctctat
      241 gagattacaa tgtacataat agttatatct atcttcttat ttttatgttg tactttatct
      301 acaattttta ataagaaaaa aaaattaaac atatatttaa aaaattccat tttaaatata
      361 ttatccattg tatcaatagt ctactttctt tttgtagtgc tttggggaat aaactataat
      421 aggattccac tagaaaccac acttataaat aactataatt taaaaaacaa tacctctatc
      481 caatcaaaac aatatagtgt taaagagctt actaaattat ataaattcct tgtaattaaa
      541 gcaaatgaaa ctagaaaact cacattacaa gataaaaatg gtattgtaaa atctaataca
      601 gattataatg gtgtcataaa tagagctcaa ctaggatacg ataatatctt ggatatatta
      661 cctggtgtta gtggtagtta ttctaagcct aagtatatta tttcatcaaa tctaatgtgc
      721 tatacaggaa taactggtat atactttcct tttacaggtg aggcaaatgt aaatatagca
      781 atacctgact tatatattcc ttgtacagtt gaacatgaaa tggcacatca aaggggattt
      841 gctagtgaag atgaggctaa ttttatagcg tatcttactt ctataaaaca ccccaatatt
      901 gattttaact attctggata tattttagct ttaaattata ctgcttctgc tttaagtaag
      961 gttgattata atgcatatgt tgatatttct gctggcattt cagattctgt ccgtagagat
     1021 ttaaaaaatg aaagtgaatt ttggcaaaaa tatgaaggta aaattaatga aatttctaat
     1081 gagtttaaca attcttactt aaaagctaat ggcgttactg aaggtactca gagttatggt
     1141 aaaatggttg atttattgct aacttattat gaattatatc catataacta aacactacaa
     1201 ttcaaaattt tattcatata cagctgatat taaatcagct gttttctatt taaataaatt
     1261 atttaacttc aagcaatctt atataatttg atagtactta tactatctgt ataacatttc
     1321 aattctaact attttagtta aaacatttct attatctttt ttaataaatg ttttaactaa
     1381 aatttttaaa acataaaact tccacattat ttcactatct atataaaaat ctaaaaattc
     1441 acatataaaa ttacataact aaaaaatcat aaatttatag aaaacatata ttcaaaataa
     1501 aaaagcatgt atcaaaactt gacacatgct tataatctta tgtatatata tcattttgtc
     1561 acaataaaaa tttacttgta acactgatat ttttaaacaa aatagtcctt atttaattaa
     1621 attaacatta attaaattcg tactcatttt gctatcatta aacattactt atagtaattt
     1681 tctaatacac tatttgaaac ttccttccct tgaaaacttt tcaaccaatt ctcatatgct
     1741 gagtttttcc atacactacc atcattaaat gttacttgtt ttacacaaga tataatgtac
     1801 tttacaccat gctctccagt tgattgtttc catttatcaa acaatagaaa ggcatcattt
     1861 tgattcttac ctggtgctat tgcattttgc tttgcatatt ctttctccat ttgtttccat
     1921 tctttcaacc aattttctac ccatgccttg ccttgttgtt tttcaaacat acttattata
     1981 tcctgtggtg gtgattgttg catttttcta taagtatgtg aatatgattt tggagacaca
     2041 ggagatatgc ctgtaacaat accatagtct acaccagctg ggctaaatcc aacacttcct
     2101 acttcgccat ctgctgccac attttgagca tcccaatata gctcaagtgg ttttccattt
     2161 ttatcatatg ctaagcatgt aatttcataa tccataattg ccttttcact gctatttgtt
     2221 aagcttataa cattgctagg ccaatgatat ggagtatcat catactgtat tttgctatta
     2281 tttataaata atggaatatt ctcattataa acaagacctt tactactttg aatatctgat
     2341 accttctttg tgacagtact taccgtattt gctaagtata catttgtttt ttctgaattg
     2401 gtcgtactaa gagaaaatgt tgttgtagtt ccagtaatca gcaaagcaac aactaaaata
     2461 ggtaagctag atattttttt atatctcaca gttcttcacc tctttttcta ttaacttata
     2521 ataaaacaat ttaccaaaac attaaaatct atgttaacat aatttaccaa ctgttctagt
     2581 aactatatcc tatcatagtt actatcaagt ttcaatacta ttgatggaaa ttttcactat
     2641 attaaatctt tttttaattt tataaattta caatatgatt taactcatac atatatagtc
     2701 aaattagaat ataagtttct tacatttaat gaagtttata aaataaaaaa tgactagata
     2761 attaaatact gtaaataaaa acttattaca aaagtattta aaaatctagt caattaatat
     2821 atatatgaat tgtatactat aatttttcaa tctcttctag caactcatca actaatttat
     2881 taccatcaat cattctaact atttcaccct ttttaaataa aagaccttca cctttacccc
     2941 cagcaatacc tatatctgcc tctctggctt caccaggtcc atttacagca cagcccataa
     3001 ttgcaacagt gatatttttt tccatactac caatcttttc ttcaacctca tttacaacac
     3061 taattaagtc tatattacat ctaccacatg tagggcatga tataacttta atcttatcat
     3121 ttaaaagtcc caaacttctt aaaatctgtt taccaacaat aacctcttca acagggtcgc
     3181 ctgttaaaga tattctaaca gtatctccaa ttcctttaag aagaagtgct cctaccccta
     3241 tagaagactt tattgttcct ttgtgaacac ttccagattc tgtaattcca atatgaagag
     3301 gataatcaac tctttttgat attaattcat atgcatcaac agttttataa atatcggatg
     3361 attttagaga tataactata ttatgaaaat ctaagtcttc aagaatctta atatgtccca
     3421 ttgcactttc aactaatgct tcagcagttg cagagccata cttttttaaa agttctttct
     3481 ctaaagaacc tccattaact ccaattctaa tttttaaatt tctttccttg catttctcaa
     3541 ctaccatctt aactctctct atacttccaa tattaccagg atttattctt attccatcca
     3601 caccttgttc tatagcttca agggctagct tataatcgaa atgtatatct gcaataattg
     3661 gtatatttac actagacttt atctttgcta tatttttagc agctttaata tcaggaacag
     3721 caactctaac tatatcacaa ccagcttcct ctaatctctt tatttgagct attgtagcat
     3781 ctgcatctct tgtatcagta tttgtcatag attgtatact tataggattt tctcctccta
     3841 tttttacagt tcctacactt acttctctcg tctttcttct ctcgtaactc atattccacc
     3901 tctatatatt attttatatt gaatctcttt aaatattaaa atattataac ctacatgtat
     3961 attataaatt aaaaagctat ctagttaaat aagaataaat ttcatatata aaaattaatt
     4021 tattcttatt taaaactaca tagcctattt tcttaaaatc tctaaaaaac taaatcattg
     4081 aaataatctt agtatatctt tatatgtgat aaaaagcata aataacatta aagctccaaa
     4141 accaacaaca tttatcatgc cttctttatt tgggtcgagt tttttaccac ctctaacagc
     4201 ttctagtaac agcataagta ttctccatcc atcaagtgct ggtattggta atagatttac
     4261 tatacctaaa tttaagctta tgacggctgc taaatacact acatttatga tgcctgtacg
     4321 agcagcatct gataccatac caataacacc tactggtcct gctacagcat tgccaatccc
     4381 tcctggtaca cgtcctgtaa tcatttgacc tagaaatgtt agcatctgtt ttgtcatatc
     4441 tacagtagtt ttacatgcat ttactattga ccccaaaaag tctttttctc tttgtggttg
     4501 aatccctatt tcatactttc cattgttatt tttaggtgtt acatttacaa ctttatcact
     4561 tccatctcta gttatactaa gttttaattc tccaccagaa gaagtattta ttatatttgc
     4621 aacttcatcc caagtcttaa cagaattacc attaatttta tttatcttat ctcctacttg
     4681 aagccctaca gcctgtgcag gagtatctgg cattatcttt cctaatttag tcgttggaga
     4741 acctatatac aaaaatactg ggactaaaag tattgctgct aatattatat tgaatattgg
     4801 tcctgctaca atagtactaa atctttgtaa tatagacttg tttccaaaag aatttggact
     4861 tatttgttct tcatcttcac cttccatgct gacatatcca ccaattggaa gtgctcttat
     4921 agaatactct gtatcttttt ttacactata tatcttaggc cccataccta tagaaaactc
     4981 atttactttg ataccacttc tttttgcaaa tataaaatgt cctaactcat gtattaatac
     5041 aattatacta aatagtatta atgcggctat tatagtcata aattcacctt cctatttttt
     5101 tatcagattt gcaacataag ctctactcca tctatctgtt tctaagattt cttctaatgt
     5161 tggttcactt atgctactat gaacttctaa agtcttctct atgtaatatg gtatgtcata
     5221 gaaaccaatt ttatcctcta aaaattcact tactaaaact tcatttgctg aatttaaaac
     5281 agaagaataa gttcctccca tttttaaaca ctcatatgcc aattttaaac atggaaaagt
     5341 ttctaaatct ggttcttcaa aagttaaagt actaaatttt gaaaaattca ttctttcaaa
     5401 gctactttcc attctatctg gatatgtaag agcatactgt attggtagtc tcatatctgg
     5461 acatcctaat tgagcaatta ttgagctatc tgtatactgc accattgaat gaattatgct
     5521 ttgtgggtga actactacat ctatattttc ttgttctact ccaaataacc atctagcttc
     5581 tatcacttca agtcctttat tcataagcgt tgaagaatca atacttattt ttcttcccat
     5641 actccaattt ggatgcttca aagcttcatt tttagttata tttgcaagtt ctcccttttt
     5701 ctttcctcta aatggaccac cagatgctgt aagtattatc ttttctatat tttttttatt
     5761 ttctccattt agacattgaa atatagcact atgttcacta tcaacaggaa gaatatttac
     5821 attgtatttt tctgcttctt tcattacaag ttcaccagca gttaccaacg tttctttatt
     5881 cgctaatgct atgtctattc ccttctttat agcacaaaga gttgggacta atcctatcat
     5941 acctactaca gcagttaaaa ggacatccac ttcatcaagt gatgatatta tttttaatcc
     6001 ttccattcct gctaaaactt ctatttctat atttttaggc aacatttctt ttaattttaa
     6061 tgctttattt tcctcaaaaa ctgttacata tttaggttta aactctacta tctgttctag
     6121 caataattca atattactgt tagctgatat tgcaactatc tcaaacttat ctctatgttc
     6181 tctaaccaca tccaaagttt gtttacctat agagcctgta gaacctaaaa ttgatatctt
     6241 tttcatataa taatcctttc taataataaa tataccctgt caaaacatta taacactact
     6301 aaaacaatta tttttactat tcatagaaat ttcatatatt attccacaga ttaaaattaa
     6361 tctattattt tttgtatttt gtggaatatt ataaaatatt ctagtattat taaatgccct
     6421 tttttatttc cacaacatct tctattttac agtctagtgc taaacatatc ttctccattg
     6481 tatccataga aacagattta tcatttccta gtctgcctaa aacagaaata cttatacctg
     6541 atatatttac aaattccatt ttctttattt ttttgtcaat taacaatttc cataatggag
     6601 cgtatgaaat catgttatca cctcgcaaaa tatattctat cacattttat ctattttgga
     6661 aaattttcat ctatttagat aaataattat ttatctaaat agatttcaat tatagtttat
     6721 taaatataaa tatgttaaaa taaaacaaaa gcataagttc aaaaattaaa ctttatatat
     6781 atttatctca ttattttact aaaacataac tatataaaaa atatttacta ttatatatct
     6841 atttaaaaca cattattata gtatcatata aacatatgta aatatataat taaaaaagct
     6901 aaagattagt aaatttataa aaattatatt ttgaataaaa ggagaaagaa tgttgtattt
     6961 actaaaaatt aatcaatttg aaaaaagtta tgatatagga atttttaaaa atgaacaatc
     7021 aatatataaa tttattgaaa aaataccctt tgtaaaaaaa gataatagta attttaatta
     7081 tacaaattac tatatg
//
LOCUS       DAFKMX010000184         7081 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFKMX010000184 DAFKMX010000000
VERSION     DAFKMX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.187"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1023
                     /locus_tag="LZB08_003161"
     CDS             <1..1023
                     /locus_tag="LZB08_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBM7316628.1"
                     /translation="ICITTYLVLTTLDIKLIPKIIRLVNKTYIFLGILTIIVYMIIES
                     IVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASGSQPMQIYALTKYDVSLSKSV
                     AIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKVLITFGMIMNVSMLLGGLLIV
                     FSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYIDDYNYSIKIFIKNVRILVLSI
                     LLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFLYMAISPIPTPGNVGANEVTF
                     FTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFGTHYTLNSWKKENKVNSNLDK
                     VIEKLN"
     gene            1172..1333
                     /locus_tag="LZB08_003162"
     CDS             1172..1333
                     /locus_tag="LZB08_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBM7316629.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1674..2942
                     /locus_tag="LZB08_003163"
     CDS             1674..2942
                     /locus_tag="LZB08_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBM7316630.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            3137..3925
                     /locus_tag="LZB08_003164"
     CDS             3137..3925
                     /locus_tag="LZB08_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBM7316631.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            3925..4374
                     /locus_tag="LZB08_003165"
     CDS             3925..4374
                     /locus_tag="LZB08_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBM7316632.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            4378..6642
                     /locus_tag="LZB08_003166"
     CDS             4378..6642
                     /locus_tag="LZB08_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7316633.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6782..>7081
                     /locus_tag="LZB08_003167"
     CDS             6782..>7081
                     /locus_tag="LZB08_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316634.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGS"
BASE COUNT         2749 a          738 c         1290 g         2304 t
ORIGIN      
        1 atatgtatta ctacttattt agtattaact actttagata taaaactaat acctaaaatt
       61 ataaggctag taaataagac ttatatattt cttggcatat tgacaattat agtatatatg
      121 ataattgaat ctattgtaac tcatttgata ataaatgcag ttcaaaaaac aaaagtaaaa
      181 tttattggta ttaaaatggc tacaatggga ttttactata acttagttac tccatttgca
      241 tctgggagtc aaccaatgca aatatatgca cttacaaaat atgatgtaag ccttagtaaa
      301 tcagtggcta tagttactaa taaaactgta gtatttcaaa gtacagttac tgtattttgt
      361 ggaattttaa ttttgctaaa taagtcatta ctgatggagc aagtacattc tgtgaaagtc
      421 ttgataactt ttggaatgat tatgaatgta agtatgttgt taggtggact actaatagta
      481 tttagtccaa aacaagtaaa aatgatagca gaagttttga ttaatttttt atctaaattt
      541 aaaacattta aatttttaga ttcaaaacgt gatagaataa atcattatat tgatgattat
      601 aattattcta taaaaatatt tattaaaaat gtaagaatct tagttttaag tatattactt
      661 actgtgttac aattaatcgc atatttcagt atagtttatt gtgtgtataa agcctcaaat
      721 ttaagaggag tttcatatgt acatataatg acattacagg tctttttata tatggctata
      781 tcaccaattc caacaccagg aaatgttgga gctaatgagg ttacattttt tactatgttc
      841 tccaatataa ttccaaaaga attattgggg tactcggtac tcttatatag tgggtttgta
      901 tactatttta tattaatagt ttcaggttta tttacatttg gaacgcatta tacattaaat
      961 tcttggaaaa aggaaaataa agtaaattct aatttagata aggttattga aaaattaaat
     1021 taataaaata attagggaaa ttctatatat aaatatggtg ttgaataagc tatattatat
     1081 tgagatattt cccttttttg attaaaatta tagaaatttg aaataataag taataaacag
     1141 aaaaagtgat attgttttga aaggagaaac tatgaaacaa gataaacaaa atgaaaaaca
     1201 aagtgagatt tctaagataa aacaaagcaa tgataataaa cttgaaatag ctcaagaatt
     1261 acatgtaagt aaaaatatag ggaatatgct tggatataga atagataaga aaaaaatgaa
     1321 tagaccaagt taatgaaaat gttgaaaatg gtacatttta aaaaatatat ataatgaaat
     1381 gttttattgt taaatttaaa cttattttaa tggaggaaaa agtcgaaaaa tatctaaaaa
     1441 gatatggata taaaaaatat taatatatat atactataaa cttgacaaaa tcaacctttt
     1501 aataactagc aaaaaatagc aaaattatat aagcgttatt taattgtcaa aaatgaaagc
     1561 taaattgcat gtcctaaaaa aaatgattga taacatttat ttgtgtgcta taattatata
     1621 gtaaaacaat aagtatattg cataatgtat gcaaatcggg aggagagacg gacatggaag
     1681 catctataca cacaattgcc agcaataatt tattgataat ttttgctatt gtaagtataa
     1741 ctggaataat ttgtagtaag cttagtgaaa taataaaggt tccagatgta gtgttatatt
     1801 tacttgtagg tattttaata ggaccatcat ttttaaaatt tatagatatt cgtggatttc
     1861 aaatagaaaa tcaactaata cttacatttg gttctgcatt tattttatat ttaggtggaa
     1921 aagaaataag tttaaaagta ttgagaaatg tgaagatatc tgtattctta ttagctactt
     1981 taggagttgt gatatctgct tttataatgc aacaagtaat aggatttact tttgggataa
     2041 gtgcaatgac tgctttacta gcaggaacta taatagcatc aacagaccca gccactcttg
     2101 tacctatatt taatcaggta aagataaagg atagggtaaa acaaactgta ataagtgaat
     2161 cagcttttaa tgatgcaaca ggagctatac taacatcagc agtgttggct gtaattttgt
     2221 caaataaatt ttcattaggt gaaaatattt atgaactggg gacaatggtt attgtcggag
     2281 ttttagttgg tttaattaca ggaatactgc ttttgaaatt agttaatgat aaaccttatg
     2341 gaatatttaa agattttgca ccaataatat ctgttatttc agttgtagtt gcatatgaat
     2401 tatctacaaa attaggtgga agtggatata tgtcttgctt tattgtaggt ataataactg
     2461 gtaacaagaa aaactttaaa atttggttaa gtcaaaaatc ttatgatgca gatttttatg
     2521 ttgctgaaac tttaggtact atttgtcgta tggcaatatt tataatattg ggtagccaag
     2581 ttaatttggc tcagttaagt aaatatttta tgccatctgt aattacagta ttagtattaa
     2641 tgtttatagc aagaccactt tgcgttttag tttgtacact tgtagacagg gaagcaaaat
     2701 ggagtaaaag tgaaatatta tttatgatgt gggtaaggga aacaggcgta ataccagcag
     2761 cactttgtgg tattatttca gcaatgaaag ttcctggata cgaagtgatt tcatctgtag
     2821 tattcatgac tatactaatt acattagtaa tacaaggaag tactacaaaa ttagtagcta
     2881 aaaaacttgg attattagaa gaagaagtga taaatataag tgaaaaagta tcaacttttt
     2941 aaagttttgc tttaaaatag tacctgttaa tggctatttt aatattgtct aatgatgatt
     3001 attgaaatag tcattttttt atgtataagt aatttacaga atttacatat tagacatatt
     3061 tattatcaaa atttgttgaa gctatgatat aattagttgt atttaaaata agaatagcaa
     3121 ataagggagg aaatttatgt ttacggttat ggatattgta cagccagata cagtagaaga
     3181 agcgtactca attttaaata aaagaaaaac taaccaagta attggtggaa gtgctttttt
     3241 aagaatgggg aaaaaaagaa taggaacagg aatagaatta tctaaattaa atttagatta
     3301 tataaaagag tatgaagatt acgttgagat tggttctatg actactttta gaacattaga
     3361 aacaagttcc ataataaaaa ataattttgg aagaataata gaagattcag ttaaaaatat
     3421 aataggagtt cagtttagaa atgtagtaac agttggtgca acagtatttt ctaaatatgg
     3481 tttttctgac ttgatagtag cattactttc attagataca gaagtagaat tatataatat
     3541 tgggagaatt agtcttgaag agtttctaaa tagagattat gagaaagatt tacttattaa
     3601 gatatacata aagaagacaa ataagaatgc ttcttataaa tcacttagaa atgcaaagag
     3661 tgattatcca attctaaatg tatctgtatc aaaacatatg gaacaattta agttatgtgt
     3721 aggagcaaga ccgcagaaag ctacgattgc aaaacaagct agtgaatttc tatctaacaa
     3781 tgaaatcaat gaaattaata tagataaagc agtagaaata gcatcagaag aactaacttt
     3841 tggttctaac atgagagcat ctagggagta tagaaaagct atgagtaaag tacttttaaa
     3901 aagagctata atggaggtta tttaatgtta gtaaagatta atataaatgg gaagaaaaaa
     3961 agtgttgata tagaaccaga agaatatctg gttgatactt taagaaaagt tggtaattta
     4021 agtgtgaaaa gaggttgtga cacaggctgt tgtggtttat gtacagtttt aattgacaag
     4081 aaacctacat tatcctgtgc aacactgaca ttgagagctt tagataaaga aataactaca
     4141 attgaaggtt tagaaaatga agttaaagaa tttagtgaag ttttggtaaa agaaggagct
     4201 gagcaatgtg gtttctgttc tcctggcttt atacttactg tacttgcaat gaaggatgag
     4261 cttgaaaatc ctacagatga tgaaataaag cattatctta caggaaatct atgtagatgt
     4321 acagggtata tgggacagtt aagagctatc aaaacttatc tggaggtaaa atagaacatg
     4381 aatatagttg gaaaaggaat caagaaaata gatggaatga gtatcacaac aggaaagcct
     4441 gtatatacag atgatttatc agcaaaagat gctttggtgg taaagatact tagaagtcct
     4501 cacgcttatg caaaaataaa agatatagat acatcaaagg cagaaatggt tgatggtgtt
     4561 gaatgtgttt taacatataa agatgtacca aaatctcgat ttacacttgc tggtcaaaca
     4621 tatccagagc catctccata tgatagatta attcttgaag atgttgtaag atatgtaggt
     4681 gacgaagtag ctatagtggc agctatagat gaaaaaactg caattaaagc aatgaatctt
     4741 atcaaagtaa agtatgaagt acttacacct gttttggatt ttgaagaggc aataggaaat
     4801 aaaactatgg tacatgatga agatagacat tgtaactttg atataggtat gcaaagagaa
     4861 aaaaatatag tatcccatca tgattataca aaaggtgatg tagaaaaagt attaagtaat
     4921 tgtgatgtag tgattgaaga aacttattat actcaagctc aagcacatgc aatgatggag
     4981 acgtataggg catacaatta tttagaccat atgggaagat tggttgtagt aagttcaact
     5041 caaataccat ttcatgtaaa aagacattta tctcgtgcac tagaaatacc agctagcaaa
     5101 attagggtta taaaacctag aattggtggc ggatttggtg gtaaacaaac tgcatgtgtt
     5161 gagatttttt ctgcaattgt tactttaaaa acagggaaac cagctaaaat aatttatgat
     5221 agaaaagaaa cttttaattg ttcaacaagt agacatgcaa tgagattaaa agttagacta
     5281 ggtgctacaa atgatggcat aataaaggca atagatataa aagcactttc tgatacagga
     5341 gcatatggag agcatgcttc tacaactatt ggtttggttg gagaaaaaac tttaccaatg
     5401 tataataaag ttgaagcaac aagatttatt gcagatgtgg tatatactaa taagatgcca
     5461 gcaggagcac ttagagggta tggagcatca caaggatgtt ttgctgttga atcaactata
     5521 aatatgcttg ctaataaatt aaaaatggac ccaacagatt taaggttaaa aaatgtggta
     5581 acagaaggtg aaactacatt tgcatatggt aaaactttaa atagtgtcga tttaataaaa
     5641 tgtataaata aaggcaaaga acttattgga tggaatgaaa aatatccttg taaacatctt
     5701 gataatggaa aaattagaag tgtgggtatg gcaattacta tgcaaggttc tggtatacaa
     5761 ggtcttgata agtcttcagc tgaaataaaa ttgaatgact gtggagatta tactcttatg
     5821 ataggttcta ctgatatggg tacaggaagt gatacgattt tagctcaaat ggctgctgag
     5881 gttcttgata caagcattga aaatataaca gttatatcag ctgatactga cttagttcca
     5941 tatgacccag gttcatatgc ttcaagtact acttatgtaa caggtatggc agtagttaag
     6001 gcagctcaag acttaagaga aaaaataatt tctgctggag caactaaact aggcgttgag
     6061 aaggatgaag tagaatttga tggtataaaa ttaactagta aaaaaggtag cactttatta
     6121 ttcgatatag cagttgattc tacaattggt ccagatacta atcaattaat aggtcttgct
     6181 tctcatggta gcccagtatc tcctccacct tttatagcag gttttgcaga aactgaaatt
     6241 gatacagaaa ctggaaaagt tgatttagta aattatgtag ctgtagtaga ttgtggaaca
     6301 gttataaata aaaatctagc taagatacaa gttgaaggtg gaattgttca agggattgga
     6361 atggcattat ttgaagaagt tagatacaca gaaaaaggaa gcatggacac aaatacattt
     6421 atgcaataca agattccagc tagatgtgat attggaaatg ttaaagtaga ttttgttgaa
     6481 acttatgaac caactggacc atttggtgct aaatctgttg gagaagttgt tataaataca
     6541 cctttaccag caatacaaga agctgtgttt aatggaattg gtgtaagaat aaatacactc
     6601 cctattacac ctgaaaaagt ttttatggaa atgaataaat aattatttat gaaaatttag
     6661 actagtttta catgttaaac aaccagttat gtcatacctg ttgaaagata tttaataagt
     6721 agttattata atattatgaa ttttacttat aaaatacaaa attaaataag gggatgatgt
     6781 tttggctggt aatctaaata atatgagagc agtaaataat tttagaggag ataagaacat
     6841 tttagaatgt ttagtcagct ttgagggtcg ttcaataagt cagagaaaag taagggtatt
     6901 ttttaaagaa aaacaaaatc aaatagaaat tgattttgca gaagaggaaa tttctaaatt
     6961 ggttgaaaat gttgttttaa atacatcata tcaagaaatg ttatatgatg aaatagagaa
     7021 acaactggaa attgattgta taggtacttg gatgatatta tctaaattaa aagatggtag
     7081 t
//
LOCUS       DAFKMX010000185         7067 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFKMX010000185 DAFKMX010000000
VERSION     DAFKMX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.188"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            69..2426
                     /locus_tag="LZB08_003168"
     CDS             69..2426
                     /locus_tag="LZB08_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBM7316635.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2512..2829)
                     /locus_tag="LZB08_003169"
     CDS             complement(2512..2829)
                     /locus_tag="LZB08_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBM7316636.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(3410..4681)
                     /locus_tag="LZB08_003170"
     CDS             complement(3410..4681)
                     /locus_tag="LZB08_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBM7316637.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   5328..7065
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5328..5356
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         2541 a          824 c         1042 g         2660 t
ORIGIN      
        1 aattgagaat tttataatat atttgtagat ttgagtataa aaaagtgcta agttgaaagg
       61 agtaaaatat ggataaggtt aatttagaaa ggtatagttg tgatataaat gttggtttaa
      121 ataaaaatca acttcaaagt agaatagatg ataatttaat taatgcattt gataatggaa
      181 aaaccaaaac atataaacaa atttttaaag acaatatatt tacacttttt aatctcataa
      241 atattgtttt agtctgttta ttagtttcag ttggttcttt taaaaatacc ttatttatat
      301 ttattgctgt aattaatact attataggag taatacaaga gataagagct aaaagattat
      361 tggacaatct atctgttata gtttcaaata aggtatctgt cataagagaa aaagagaaaa
      421 aagagattga tgtacaagaa ttagtattag atgatattat gattttaaaa acaggtaatc
      481 aaatttgtgc agattcaaaa gttttaaatg gaaaattaga ggtaaatgag tctttggtta
      541 caggtgaatc tgatattatt ataaaaaata aaggcgattt tttatattct ggaagttttg
      601 tagtttctgg agaagctttt gtaagagttg aaaatatagg aaaagataac tatgcaaata
      661 agatagctaa tgatgcaaag atttctaaaa aacataattc tcaacttaga aattcattaa
      721 atgcaatatt aaaaattgta ggtataataa taattccttt aggaattata ttgtttgcaa
      781 aacaacattt tggaaatgga gattctatag ctacatctgt agtaggtaca gtggcagctg
      841 taaatggtat gataccagag ggattaacat tacttacgag cattgctcta gctgtaggaa
      901 ctataaaact tgctagtcat aaaactttag tgcaagaact gtactgtgta gagactcttg
      961 ctagagttga tacactatgt cttgataaga caggtacaat tacagaaggt aaaatgcaag
     1021 ttgatgatat agtaatgttg gaagaagtgg atattaatga aataatgggg aatatatgta
     1081 attctctcaa agatgataat gcaacactaa atgcaataag agataagtat aatgttttag
     1141 atacgtataa agctaagaat ttaataccat tttcttcaga aagaaaatac agtggggtta
     1201 cctttgaaaa taaaggtaca tatttattag gtgcatttga atttatattt aaggaaaaaa
     1261 ataaaaaact taggatagaa gttgaaagat attctaaaaa aggaaacaga gtaatagttt
     1321 tagctcatag tgattcttat atggaagata ataatattcc tgaagatata aagccactag
     1381 cttttatatt gatattggat aaaattagag atgaagctaa ggaaacttta gaattttttt
     1441 acaatgaagg tgtagatata aaaattatat ctggtgacaa tcctataaca gtaagtgagg
     1501 tagctagaaa agcaggtctt aaaacagcta gtaattttat tgatgcaaca acacttaaaa
     1561 atgatagtga tatatacaaa gcagttgata aatacagtgt ttttggaagg gtaagcccac
     1621 aacagaaaaa acaaatgatt ttagcattaa aaaaacaaaa tcatacagta gctatgactg
     1681 gagatggtgt aaatgatgtg ctagcactta aagaagcgga ttgtagcatt gccatggcat
     1741 ctggaagtga tgttactaag aatgtgtcta atttagtctt attagattca aactttgcat
     1801 caatgccaaa ggttgttatg gagggaagaa aagtaattaa taacattcaa agagcatctt
     1861 ctttgttttt agtaaagaca attttttcat tcttcttgtc gttattaaca ttattcttct
     1921 tcagtagaca gtatccattt gttcctatac aattatcact cattggggct gtaacagtgg
     1981 gtataccatc attcttctta gctcttgaag caaacaaaag tagaatttca ggaaattttt
     2041 tactcaatat attaaaaaat gctttaccag gagcactatg tgtgacagta aatgtaatta
     2101 ttgtttacaa tatagttgaa tatttaggtt atagttcaag tgtattttcc acaatgtgtg
     2161 ctatattaac tggtgtatgt ggtcttttaa tattaagaca acaatgtttg ccatttaata
     2221 aagaaagatt atttttaata gtaagtatga ctattgcatt tgttattggg attctatttt
     2281 taggtggatt attctcattt gtagcattga gtagagaatt aattctcata actatggcat
     2341 tagcaatatt aatgccaatt atgataaaag ttatgttatt tgtcttagat gagatattag
     2401 agacaattgt tccagattta aattaatttt aaaatgatat aaataagagc agttttagtt
     2461 gaactgctct ttttagatgt gtttaatttt atgtaagaat atgtattttt attaatcagc
     2521 tatatatgag caacagtagt caacaacttc attaaccttt aagtcaaaac ttgaatcact
     2581 aggaacatta aatttttgcc cttctttata agttacaaac tcattgcttc caggcagttt
     2641 tacatccata cttccagcaa gcatctccat tatttcttct tctctagtag aaaaagtgta
     2701 ttctcctggc aacattaatc ctaaagtttt tctttctcca ttaggcagta ttattactct
     2761 gctacttacc ttaccatcaa aataaacgtt tgccttcttt actgcagtaa cattcttaaa
     2821 ttcactcatt agataaaacc tccaaaatta tttgtacata tatattatat aaaaataatg
     2881 taaaaaatgc tatatattta caaaaaaatt tagtatttat taaaaaaatt aataagaata
     2941 aaaaagagat gaaattgctt aaattgataa aaaatataaa ataaattgca aagtaagcaa
     3001 gaaaagaata agttgaagag ttatatatta taaatattaa aaattttagt attaataaaa
     3061 aagatattac attaatatga aaatatagga gattagaagt gtttgataat tacagtacat
     3121 gttagatata tcattgtaaa ttaacataac tttgaatata ataatgttca gaaggcaata
     3181 tttttgaggt atgaatccaa taatatagtc ttatgattgt agatatcaaa aaatataagt
     3241 tactaggaat acgttttgta gatttatata tgattaataa ctaaagctat aaataaacaa
     3301 aataaaaaga gtatgtttca acctatagct tttagattta aaatatactc ttttattaat
     3361 tatagttgta taaattaacc ataatatata attaaaattt ttattataat tatatattag
     3421 gttcttcaat tagttttcca catacatatc tatgaatgat atttctatca tcaccaacat
     3481 atataaatct ttcaagtctt tcaagtatag aataattgtc atgatttaaa tttgaatcat
     3541 caataatcaa tgcatcaaaa tcataacctt cttcaaagct accaactttt ccaaagaaac
     3601 ttccaccact tttggttgca atataaaaag cctcagataa agataagaaa ttatacttct
     3661 taccagagtt aacccaatat aatttagata attgtattgc agataccatt gctttaaata
     3721 ttgacaatgt atgtccacca cttatatctg atccaagtgc taatctaata ttattatcga
     3781 ttaattttct gataggcata gcaccactac caacattaaa gttagaagct ggacagtgaa
     3841 ctgccataac attattttct ctcattaaat ctatttcatc ctgagaacaa tgaacacaat
     3901 gagccattaa agtaggagtt tgaccaaata aattatatct attatatgca tctccataga
     3961 atttagattc aggttctaga cttctaaccc attcaatctc atcataattt tcagataagt
     4021 gtgattgaat aggaatatta tattttaaac ataaatctcc taaacctttt aaaagttcac
     4081 ttgtacagct tggtataaat ctaggtgtta ttattggttt tacaatagaa tctgtatcac
     4141 tatacttatt tataatttct tctgtatcag taatagaatc ctgagtgttt tctaataaag
     4201 tatcagggca attcatattc atatttactt taccaacgta tgctccaaga ccaacttctt
     4261 tgaatatatc cataagtttc atagaggctt ctttatgtac tgttgcaaac actgctattc
     4321 ttgtagttcc agatttccaa agttctttta caaatttttt atacattttt gttgcatatt
     4381 ctatatcttt ataatttgat tcttctggaa atgtataagt ttcaagccat ggcaaaagct
     4441 ctttatccat agccattcca agatttttaa attgagaggc atgggcatgg aggtcattca
     4501 taccaggtat tataagctta tcagtgtaat ctactacttt taggtttttg tattccttag
     4561 gtatctcttt aaatattccc tttactttac ctttttccag tataatataa ctattttcaa
     4621 atactgtgaa agtttctgaa gtttttgtaa aaattatatt tccttttaat acttttaaca
     4681 tataggtctc cttagtttta aatttgtttt aatatatgta ggtaaaattt tgttttacaa
     4741 atcaaaattt taccacatgt attatatcaa atctaaacta taaatactat tagataatag
     4801 caaatattag tgaaatacag ttgtttacta ttattatata taactgacat ttaagtgaca
     4861 tttaagaaaa atataatgcc tacttacata aaatggaatg ttatttaaag agaactttga
     4921 ttatattttc agaagctttt ttatccatat cgtttaaaag atgagaatat ctatttatag
     4981 ttatttttat attagtatgt cctaatcttt cagatatgat ttttatatta gttctagcta
     5041 gaagaagaac tattagaata gataatatag taagtattta caaatatgta ggtgttctta
     5101 aattgataaa ttattccatt ttaattttat agtttgaatt ttatgatata ataaaaatat
     5161 ataaattttg cagtgagcga tatttttgat aaagtagggt ttaacagttg caatgtaagg
     5221 gattgagggt gtgtgataaa tgttatcaat tgcactactc atggttcact gcaaatttga
     5281 gagagttgta tgtgtgtaag tactgaaaat acttagttta ttttggggtt ttatattaac
     5341 taagtggtat gtaaatgaat tattaggaac ataaacatcc aatccatctt ctgttttata
     5401 ttaactaagt ggtatgtaaa ttttctaaca tcctgtacag tcgtttcttg ccatgcgggt
     5461 tttatattaa ctaagtggta tgtaaatcga tttagctcta ccaacaacaa ttacagactg
     5521 cgaagtttta tattaactaa gtggtatgta aattattaca aatccaactg ttatacctaa
     5581 tgcactcgtg ttttatatta actaagtggt atgtaaataa tactttatct attttcaatc
     5641 ttttacttgc ttcgttttat attaactaag tggtatgtaa attagcagta tttagtgtgt
     5701 gggctagtcg tgttttcgat agttttatat taactaagtg gtatgtaaat ttaaaatact
     5761 ttttttaact ccgtcaactt caacatcgtt ttatattaac taagtggtat gtaaatgtgc
     5821 aaataatagt gccaagtttg gtggtataac attgttttat attaactaag tggtatgtaa
     5881 ataaagaggt gatgtctact tttagctttg aaacatagtt ttatattaac taagtggtat
     5941 gtaaataaat ttttagcact ctacaccatc tctaaatatt tgggttttat attaactaag
     6001 tggtatgtaa atgtctttta taactaggtc tattgagcta ttatttgagt tttatattaa
     6061 ctaagtggta tgtaaatgat aattgctatt ttactaaagg tacggtcgtg atttgtttta
     6121 tattaactaa gtggtatgta aattcgctag gttttatatc tgctttatta gtatttttaa
     6181 tgttttatat taactaagtg gtatgtaaat tatattttag atgttaatgt tgcaaataca
     6241 aggttcacgt tttatattaa ctaagtggta tgtaaataag ctccaaaata gctataattg
     6301 ggaacagctc ctttgtttta tattaactaa gtggtatgta aattgattta ctgttagacc
     6361 actcctgctt atctctacag ttttatatta actaagtggt atgtaaattc atccagcaat
     6421 ttttttatta tagctttcat gggtgtttta tattaactaa gtggtatgta aatgttatct
     6481 tcgccaattg ggttagttga acaaattata gttttatatt aactaagtgg tatgtaaatt
     6541 attttttcaa ctcataattt gcacacttaa cctctagttt tatattaact aagtggtatg
     6601 taaataaact aatcaccacc ccgattactc aatttataag tgttttatat taactaagtg
     6661 gtatgtaaat catggacttc tttgaagatt ttacaaggtc aacaatagtt ttatattaac
     6721 taagtggtat gtaaatctca gtatgtattt atagtgcttt tgtcagttgt atggttttat
     6781 attaactaag tggtatgtaa atttagaaat tttacttgaa aatattatac taaaggatgt
     6841 tttatattaa ctaagtggta tgtaaatact cgactgaaca tagaaacagc ttttacaact
     6901 ccaagtttta tattaactat gaggtatgta aatcttatac ttagttagaa ctatatatcg
     6961 acacaaatat gctttatatt aactatatgg tatataaatt gcaattttta ttcgttgtcc
     7021 aatctctttg aaatttgttt tatattaact atgtggtatg taaattt
//
LOCUS       DAFKMX010000186         7051 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFKMX010000186 DAFKMX010000000
VERSION     DAFKMX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.189"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            114..1502
                     /locus_tag="LZB08_003171"
     CDS             114..1502
                     /locus_tag="LZB08_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316638.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVIQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            1530..2483
                     /locus_tag="LZB08_003172"
     CDS             1530..2483
                     /locus_tag="LZB08_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBM7316639.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            2543..2767
                     /locus_tag="LZB08_003173"
     CDS             2543..2767
                     /locus_tag="LZB08_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316640.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            2760..3272
                     /locus_tag="LZB08_003174"
     CDS             2760..3272
                     /locus_tag="LZB08_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBM7316641.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            3304..4158
                     /locus_tag="LZB08_003175"
     CDS             3304..4158
                     /locus_tag="LZB08_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBM7316642.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            4692..5486
                     /locus_tag="LZB08_003176"
     CDS             4692..5486
                     /locus_tag="LZB08_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7316643.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            5590..6396
                     /locus_tag="LZB08_003177"
     CDS             5590..6396
                     /locus_tag="LZB08_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7316644.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIKLINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            6458..6979
                     /locus_tag="LZB08_003178"
     CDS             6458..6979
                     /locus_tag="LZB08_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBM7316645.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
BASE COUNT         2712 a          789 c         1324 g         2226 t
ORIGIN      
        1 ttaataagaa taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct
       61 cataaaatga aagtatatca attgataata ctttaaataa gaagggagaa aaaatggaag
      121 ataaatttta tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt
      181 cctttaataa cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg
      241 aaaaatacta tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta
      301 gcgatattac agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta
      361 aagagtatcc tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag
      421 caatgagttg tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta
      481 agtgtgaagc aggaattaga atatacagtt taaaagaaga ccctttaaat cctaagtttt
      541 taggatattg ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg
      601 gtggaagata tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg
      661 tcatagatat aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc
      721 aatatgcaga tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat
      781 ttatggataa aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg
      841 gttatggagg agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct
      901 taggtgaatt gccatttacg cctgcatttt ctagtagact tgcaggtgca agaactcata
      961 cagcattacc attgccagga agagatttag tcgttattca aaatgaggga gaaagattcc
     1021 agttctttaa accagataac attacagatg ttcaagctat gaataatata catatggttg
     1081 atgttagtga cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa
     1141 aagatttccc ttatcctaac tttaatgttg cgggattagg aaaaccaggg ccatttggtc
     1201 cacataatct tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag
     1261 tatattgctg ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata
     1321 tcaaagagct agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt
     1381 tcccaggatt cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag
     1441 gctacatcat catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt
     1501 aaataaatac taaagaggga gagttgaaaa tgaaaatact aggtatttct tttggaacaa
     1561 aaaatggaaa taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg
     1621 ctgaagtaga atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg
     1681 cgtgtgttaa aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg
     1741 aatggttgct tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg
     1801 aagaaggggc atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata
     1861 gaggaaataa cattatagga acaaaagttg cagaagctat aggaggaaaa attccagatt
     1921 caagaatgct taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta
     1981 caagagttca atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata
     2041 atgcttggtt cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc
     2101 atcaaattgg tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag
     2161 gagaagaagg tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta
     2221 aaaaggcaat ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa
     2281 ctatatttag ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt
     2341 ttatacatgg tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac
     2401 aagaatttaa agaaagaaaa acatcttata attttataga gacattaact aaagaaaagc
     2461 aagctggagt ttcattagga taataattaa aatactaaga ctctttttta agggatgtta
     2521 gatgttatag gaggcttatt gtatgaatga aaagccaagt tttaaacaaa ttgaagttat
     2581 tttagcagtt atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat
     2641 tgttatattt ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat
     2701 agcaattaag ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa
     2761 tggatagaaa gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt
     2821 tatgggcagt attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg
     2881 caggattata tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt
     2941 tttggtgtgg agatttatgg gcagtgttag ccattttaat tatgggatat atgaatttca
     3001 atcaaaacct agaattattt ttaacactta gtatattagg tttttttgca gttgtaattg
     3061 catccttatt tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc
     3121 taactattat gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg
     3181 tagctatggc agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata
     3241 ttttgagtaa agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg
     3301 agaatggcta tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc
     3361 aaggaagaat atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt
     3421 attagcactt ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca
     3481 aatgaaggtg agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta
     3541 gatgaacata tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt
     3601 ctacgttggc aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgcagaa
     3661 agtggagatg caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc
     3721 tttgttccaa taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga
     3781 ccaaactatc gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga
     3841 gaaaaatggt tctatgatga ggtagtgcca tattttacaa actgttgcta tgttaatcgt
     3901 tttgtcagta gtaaaataat gattaattat ggagcaactg cttttgaccg tgtgtcagaa
     3961 ctatggtttg aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaagtcgttt
     4021 attaaaaaac cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat
     4081 atcgcatcag tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga
     4141 tatataccaa tgagataaaa attatagttt gtgataagta gagaaggcat ttactcttac
     4201 tggtagaaga agacttattg tatccttaaa agtaggtgaa tatatagttt tatatttaaa
     4261 tttatataaa actaagacta ataaatatga aacaatagaa ttagaataat ttgataatat
     4321 ttgttaaaag agcatttaga atgtgtttaa agattaagat attcctttta tggtagcaga
     4381 tagttgaaat aataaaactg ttatcatgaa aggaatattt ttatgtatag gaaattttta
     4441 aaaataaaag ttaaatatgt ttaagaacat cttaaagtga aagaatcatt gttagaaata
     4501 tgtagacaat atagtcttta taacgtatca tattaacaag tttacacttg gattatgaaa
     4561 taaaaagagt aattccaaag gttgtaaata ctatgctgac tttgatactt caaagtttag
     4621 gtgattttgt aattgttaat agaaatacgt ttaatgagca taaagaattt atctaataat
     4681 aggagaatat aatgtataca attggacaag tatctaaatt tttaggaata tctagagata
     4741 cactaaaatt ttatgaggac aaaggattag taaatcctaa gaaaaatgat gaaaatggat
     4801 atagaatata taatcaggtt gatatctatg atattgcaac aataaacttt tatagagaaa
     4861 ttgatataga aattaaaaag attcaagaaa taagaaaaag taagagtata aacaatttag
     4921 aactcttact agaagagaag gaacaaatca tactaaaaga gatagaatat aaaaagcttc
     4981 tcttaaaaaa ggttcagtta gtcaaagaag attgtgacaa aatcaaaaca tattttggaa
     5041 aatacacaat aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa catgttacta
     5101 actatgagga atatgaagcc ttaaaagaga atacaaacaa cttaaaaaaa gcagttacat
     5161 taactgattt aagaagagta ataaatttta atgaagaagg tataatagaa gaaaaattta
     5221 taattgtaag ggaagtagat gactacaaca aaatcataga aggtgaagtt ctatctcatc
     5281 ccaattgtat ttatactatt attgaagatg gcagatggtc aactggagga gaaaatattg
     5341 ataataaagt ggaagacaga ttaagagaaa tagcaataga aaatgaatgt gaattacttg
     5401 gaatagtcta tgttaatata ttgcttacta cttatgaaga agggattgag cgtatatttt
     5461 tagagattta tgcacctata aaataatata aaatcattga cctgggactt actcataggt
     5521 ttaaactcat gatataagca gttataactt atatagcaat taaatattaa tttgaaatga
     5581 ggattactta tgagaacaga gaaatcatta aaaaggccta tagccagttt tattattcta
     5641 acaaatataa tttttttacc acttttttta ttggtagtag tgactataat gttaaaactt
     5701 ccaacagtga tatctgacat tgcgttgtgt atatcagcgt ggtcatccac ttttgcattt
     5761 atgatattat ttaaaaaaat ttatccaggt aagaaatttt tagtacacgt aaaagataga
     5821 tttagaaata aacttaaatt ttcaacagta tctataataa ttagtataca agtcctaata
     5881 gctgtagtag ttatatttat attagcatct aaaaatcatg gaataatgcc taactttaca
     5941 gtttcatcat ggggagcatt tatatattta tttttaaaaa atttatttgc aggatcatta
     6001 ggtgaggaat taggttggag aggatttgtt caaaatcatc ttcagaaaag acattctcct
     6061 ttaaaagcat ccataatagt aggtttttgg tgggggatgt ggcatttacc aatatggttt
     6121 acaacaggat tcaaagggct tgatttaata aaatatatta tattttttat gattgcaatt
     6181 atatcaatca gtattataat gacaactttt tataatctaa ataaaacttt gataatacct
     6241 attgtaatac atcaactatt taatttttta atagggataa ttaatggaga cttaataaag
     6301 cttataaatt actatgcaat gttatattta atatttgcaa ttgcattaat agttataaat
     6361 ccaggaaatg tattatataa aaatattaga gagtaatata tatctttatg gtaagatata
     6421 aagaaattat tagaagaaca tccaaggagg aataaacatg gttttactga ttgttgatgc
     6481 acaaaagtta ataacaaacg agaggttata taaatttaat gaatttgtag taaatgtaga
     6541 aaatttaatt gatacagcta gaaaaaataa tatagaagta atatatgtac gtcatgatga
     6601 tggagttgaa aatgaattga ctaaaggtaa aaatggcttt gaaatatacg aaaaatttaa
     6661 accatgtaat ggagaaaaaa tatttgataa aaaagtaaat agtgctttta aagaaactgg
     6721 tctattagaa tacctaacaa ataatggtga aaaagatatt attattgctg gtctccaaac
     6781 agattattgt attgatgcca ctataaaatg tggatttgaa catggattta atataatagt
     6841 tccagcttat tcaaatacaa ctgttgacaa tttttttatg tcagcagaac aatcatataa
     6901 atactacaat gaatttatat ggaatgaaag atatgcgaag tgtatttcat tagaggaaac
     6961 acttagaaaa atgaaataaa aatagagtat gaattgtata tgtacataag ttgatttcat
     7021 aaaaaaggat gttaaattcc aagttaaaaa t
//
LOCUS       DAFKMX010000187         7019 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFKMX010000187 DAFKMX010000000
VERSION     DAFKMX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.190"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            218..1960
                     /locus_tag="LZB08_003179"
     CDS             218..1960
                     /locus_tag="LZB08_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBM7316646.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYRELSSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2124..2318)
                     /locus_tag="LZB08_003180"
     CDS             complement(2124..2318)
                     /locus_tag="LZB08_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316647.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2315..2896)
                     /locus_tag="LZB08_003181"
     CDS             complement(2315..2896)
                     /locus_tag="LZB08_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316648.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            3064..3882
                     /locus_tag="LZB08_003182"
     CDS             3064..3882
                     /locus_tag="LZB08_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7316649.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4892..5713
                     /locus_tag="LZB08_003183"
     CDS             4892..5713
                     /locus_tag="LZB08_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBM7316650.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(6023..6802)
                     /locus_tag="LZB08_003184"
     CDS             complement(6023..6802)
                     /locus_tag="LZB08_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBM7316651.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2621 a          924 c          893 g         2581 t
ORIGIN      
        1 atataatagg tctaaaacta tcatagcttt tactattttg tacttaaaat ttatttatca
       61 ttttaccagt ataaatttca tatacttttt tgaagttaaa ttttttatat tatttataat
      121 cttatctata tctttatgtt aaattttctt aacatgcatt ttttatattt aatatagtaa
      181 aataattaat aactatatcc atgattggag gatttttatg gttaaatcca agaagaaaat
      241 atttgttttt ctaattttaa ttgcagtttt tattatattt gcatcataca taagaaatta
      301 tactaatgat acaaaaaaag attctgttag aggaactatt ccttttgaaa aaatggaata
      361 taagcgtcct aatatacagt caatttgcga aaacattaac aagtgtaacg acaaactttt
      421 aacagctaaa acagctaagg aacaattaaa tttatttaat gaagttgata aaatttatca
      481 ggatttttac agtacactta ctatagcaaa aattagaaat aacatagatt catcagacga
      541 attttactct aaggaatata agtatcttat gagtaaatca gtagatgttg acatgtcata
      601 caaagacttt caagatagat ttgtaacttc taaattctct aaagaattag aaaaagaact
      661 tggaaaggat acatttaatt atctaaagaa cattggaaaa cttaattcta aagaagttga
      721 aggtttgctt aaaaaagagc aagacctagt tgtaaaatat gaagatttat tagctaaatc
      781 tacagtatca atagatggta tagaggtaga ctttgaagaa gccttgtcaa gaccaaatct
      841 tagccctgaa gaatatgtga aaatttattc agattactta aaaaaataca atcctatatt
      901 tggtaacata ttcttagaac ttatacagac aagaactgaa atagcaagta agcaaggatt
      961 taagaattat atagattatg cctatatgaa cctaaacaaa gactattctc aagaagaagc
     1021 taaaaaattt agacaagatg taaaagatta catagttcca ttatatagag agctttcatc
     1081 taaaccatct gattcttcta tttatattaa agtatataaa aatcgctcat ttaggaagtt
     1141 tgataaagta cttgaagata tatctccaaa actaaaagag agcttcgact atatgaaaaa
     1201 atatgacttg tacgattatt cttctggaaa aaacaaatca cctggaggat atactactta
     1261 tataaataaa tataaagcac ctttcttgtt taatacatgg gacaattctt ttttaggtgt
     1321 tactagtttt gctcatgagt ttggtcattt ttataattac tacaacagta ttaacttaaa
     1381 taataagatg caaccaagta tagatgtatg tgaagttcat tcaacatctt tagaaattct
     1441 attttataaa tattttgatg atttttttgg taaacaatct gaagctataa aaaaagaaca
     1501 cttatcgatt gttttaaata ctattattga tgcttgtctt tatgatgaat ttcaagaaat
     1561 tatatacaag aatccatata tgagtctaaa tgatatcaat aaattgtttt ttgatttaga
     1621 ggaagaatat ggagtttcca ataatatact tagagaaaaa aatgctcctt tttggatact
     1681 tgtatctcat aacttccaag ttccttttta ttacttgagt tatggtcttg catctgatgt
     1741 atcacttcag atatggcaat tatcacaaga agattacaga aaagctgttg atgtatatat
     1801 ggatttttta aatcaaaata ctgatgctgg atttaaagat gtggtagaaa aggtaaattt
     1861 acaattacct ttccaaaatg gaaatttaga ggaaataagc tctactttat atgattattt
     1921 tggtatagat aatccacttg aactaaaaaa tgcttcttaa tttctaatac tttatactca
     1981 ttcttaagat ttagtattgg attgaaataa gtgatatttt ttcacacaca attaatgttt
     2041 aataaaatta tatggtaaag tatcagaaat ttttaagact cttgttttta taatttctga
     2101 tactttattt atcaataata ttattatttt tttatatata taattaaaag gattgtggaa
     2161 atcaataaaa atatccctgt aaaaatagca ctattcatat ggaacttaat agaacttgca
     2221 tctgttacct tattattctt aatccagaac ccacaaacca tagttgaaag aaaaagtaaa
     2281 acagtgataa gacttgaacc aatagttata attttcatct tacatcaaca tcctctccaa
     2341 taatattttc tattaaaata tcaatgaatt tatctcgctc atatttattc tttatattta
     2401 taccactgta taactgaaat gattctgtag ataaagcaga aatctgtatt ggtaaaataa
     2461 tttgcagggc aatgattctt agactcattt catctttttt atttccaaaa attttacgaa
     2521 gcatagggac aatatcaagt tctgcttgca tatctccatt ggttatacat tgattcaaca
     2581 caaaaggaag cactttttca taatgtaatc cagtatcaca caattttttt aataaatttt
     2641 tcaaatcttc aataggtctt agagtatata cactatcgtc atatagctct gctattatat
     2701 ttgacatcac atctcctatg gcagtactca acaaattatc ctttgtttta aaatgataat
     2761 ttattaaacc aactccaaca tttgcacgct cagcaatttt tctaactgta atctcctcaa
     2821 tatcatctac ttcatttaaa agattcaatg taacttcaat tatacgttct ctagcatctt
     2881 tatcattact tttcataata gacctccaaa acaattaaca tttttggttc tgaacatgtt
     2941 cagtataata tttttttcat catatgtcaa tataatttaa ttataatttt agttgactct
     3001 aacattatga tatggtttat attaatatta agctaaaagt acctttataa aggagacaaa
     3061 aacatgttta agataggcga tttttcaaag ctaagtaaaa ttagtattcg tatgttacgt
     3121 cattatgatg aaataggact tttaactcct tctcatacga ataaaactaa tggatataga
     3181 tactatagtg cagaccaact ttctactaca aacagaatcc atgcactaaa agatatgggc
     3241 tttggcttgt attctataaa agaaattttg acagaatata acgataaaga aagtctcata
     3301 aaatatctaa atatccacca ttctcaagta aaagaacaat tagaagatac acagaaaaaa
     3361 ctattaaaaa tagaaaccac tattaaaaga attggaggaa atgatattat gaaaaattat
     3421 gatgtaacta taaagaattt tgcaccaaaa tatatgatga cacttagaag ggttatacca
     3481 acttatcaag acgaaggaat gttatggcat caagcatttt tagagacaaa agaccagaat
     3541 gttcaaattg aacccccaaa gtattctaaa gcagtttttt atgatactgg atataaagaa
     3601 gattatgtag atgttgaagt acaagttgct gtatctggaa agtataaaga tactgaacat
     3661 gttaaattta agactgttcc aagtgtgact gctgcaactg caattgttaa tggaaatttc
     3721 aatcaagttg cagacgcttg tgaggctatt ggtaattgga tatcagataa taactatgat
     3781 gtagatggtc ctatgtttaa tatttatcat gtaagtcctg gcaacgatag taatcctgat
     3841 aattgggtaa cagaggtatg cttcccagtt aaaaagaagt aatttttata ttaattggat
     3901 aatattttaa tttgttagac aaattacttt tagtgttatg tataaatacc tacaatttga
     3961 ttaggatatt atcttaattt gttaaacaaa ttacttttag tgttatgtat aaatatctac
     4021 aatttaatta ggatattatc ttaatttgtc agataaatca cttgtgatgt tatgtttgaa
     4081 tgactagaat ttaattagga tattatctta atttgacaga taaatcactt gtgatgttat
     4141 gtttaaatac ctacaattta attaggatat tatcttaatt tgacagataa atcacttgtg
     4201 atgttgtgtt taaatgacta gaatttgatt aggatagtat cttaatttgt cagataaatt
     4261 atttataatg ttatgtttaa atacctacaa tttaattagg atattatctc aatttgtcag
     4321 ataaatcaat tatgatttta tgtttaaatg actagaattt gattaggata ttatcttaat
     4381 ttgtcagata aatcaattat aagattatat actctcctta tactctagtt aaatatattt
     4441 taatcactaa tataaagtag tttttttaac aaaacaataa aagagtaaag tacaaataat
     4501 tcctttgtgc tctactcttt ttaactttct ttagacaatg atataatgat aaatataaaa
     4561 aattatatat tccattctat agaaatatgc tcaccaataa catgtatgtt gttaatcaat
     4621 atattatatc aaattatatc aaaattacat aaaccttata ggacaatcat atcttattac
     4681 aagaaaaatg tcttatccat attgaataag tcattctgtt catatacaaa gtaagtttca
     4741 ttgataaata tttaatatat gcttataaaa atttcatact attctttcat atttacatgt
     4801 gatataattt attaactcat aattttgtta atcagttgtg cttgtatatt atttatataa
     4861 tctaatattt taagaaggtg gtgtaataac tttgaaatta aaaattgttg gtagtggcgg
     4921 tatgtcaatt attccaagtt cattttgtaa atgcaaaata tgtgaagaag ccagacaaaa
     4981 aggcggaaga tatgaacgat tagggccaag tttatatatt gatgatataa aaatgctgat
     5041 agacacacct gaagatattg cagtagcttg taacagacaa aaaatatcgg aagtaaaaca
     5101 tatttcaatt tctcaccatg acccagacca tgttaagggt attagaattg tcgaaaaaat
     5161 aggatgtgac tttatcacag gagaaaataa tccaattggc ttttatgcgc ttcctgaagt
     5221 aattgaagat ataaaccgaa cgaatctcga ttgtcttaaa tattatggtg atgttataaa
     5281 ttgcatttct attaatgaaa cttcgcatgt aaagataaat aattttgata ttgatttgat
     5341 taacaataat ccagacagga atataacctt ctatgttatc aaagagaatg aaaaaaaagt
     5401 catatatgcc tgctgtaatc ccaagccatt tactcatagt gatatgtact ttgatgctga
     5461 tattttaata ataagtctag tttctgatga tggtatatta aatgatggta caaaacttaa
     5521 agacacacca tttaaagatg aaatatttac tttagatgag attgttgaaa ttaaaaacta
     5581 ttatagaatt aaaaagatta ttgttacaca tattgacgaa atgtggggaa aatcatatgg
     5641 ttattacagt gaattagaaa agaaacttga taacatttat tttgcatatg atggtatgga
     5701 aattatagtc taaatcatta agtaataagc ataatgcaaa ataagaaaac ttcaatttaa
     5761 cagtatagtt aaactatgta atatcaacat atctcagttt ttacaaaata tcatagtatt
     5821 tatctttata aaatttcaag tcctacatat tttaatactt caatatctaa ataaagtaaa
     5881 atcaaaaata tattttatat acattataga cggcttatcg gcactagata agccgttttg
     5941 tcacaattat tatttgctca acaaaacttc tcataatata taacagattg aatctccaac
     6001 tgatacttaa ttaccgaaac tcttattcct taatatgttg ttgcattaac caaaccttca
     6061 tcatcatttt ttgtggcaat agttttgcta ataaatgact ggattttgta tttataccat
     6121 acacagaaat atccttattt ttctttgcat ccttcaatgc ttttttggca acaatgtctg
     6181 gagacactat gcctgggaaa ttttttgtcc ctttttcagc tacaactata cctctcttaa
     6241 atagatttgt actcatccaa cctggacata cagcaattgc atttatccct ttactcttaa
     6301 gttcaacatt taatgctctt gtataatttc ttataaacgc tttagttgaa ctataaatat
     6361 tttgatatgg caatggctgg aatgatgctt gtgaagaaat attcatgata tggcttccct
     6421 tttgcatata tggaatacat ataagtccca atgctacaac agcactaata tttaaatcaa
     6481 tcatattaat agattcatct atacttaaat cattgtatga acagaatttt gcaaaaccag
     6541 cattgtttat aaggtatttt atacatacat tttctttact taaaaatgta ccaatttctt
     6601 ttacttcttc tatttttgat aaatcttttg aaaaaatttt taccttactt ccaaattcat
     6661 ctactaattg atttaatctc gccttgtttc ttgcaactgc ccatatttct gttatttctt
     6721 cttcatttat taataacttt agaaactctt taccaaaacc actattagca cccgttataa
     6781 ttgcaatctt cttataactc ataattattc tcctttacca taagtttata cgctcaaact
     6841 gtaaacttat tttttacaaa taaaacaccc tctattcaga tgttatattc atatcatttg
     6901 agatattata cttatatcat tttcaataat tgttatatag cataaaaaat agtgttaata
     6961 ttatagaatc ggtattgatt atatttaaaa agtagtgtta attatattat agaatcgat
//
LOCUS       DAFKMX010000188         6984 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFKMX010000188 DAFKMX010000000
VERSION     DAFKMX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.191"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     repeat_region   2..1275
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=68..96
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(2106..2978)
                     /locus_tag="LZB08_003185"
     CDS             complement(2106..2978)
                     /locus_tag="LZB08_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBM7316652.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(3372..3950)
                     /locus_tag="LZB08_003186"
     CDS             complement(3372..3950)
                     /locus_tag="LZB08_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBM7316653.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(4008..4724)
                     /locus_tag="LZB08_003187"
     CDS             complement(4008..4724)
                     /locus_tag="LZB08_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBM7316654.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4915..6000
                     /locus_tag="LZB08_003188"
     CDS             4915..6000
                     /locus_tag="LZB08_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBM7316655.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(6155..6814)
                     /locus_tag="LZB08_003189"
     CDS             complement(6155..6814)
                     /locus_tag="LZB08_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7316656.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
BASE COUNT         2299 a         1084 c          717 g         2884 t
ORIGIN      
        1 catttatatt acatatagtt aatataaaac taatagttaa tgaagcattg caataggggg
       61 gtattgtatt tacattccat atagttaata taaaacccaa tgtaataaat gaccatgtgc
      121 gagaagagga aaaatttaca ttccatatag ttaatataaa acttttcatt ggcaccaata
      181 cttctaaatg ttccaatatt tacattccat atagttaata taaaaccttt gaaaaagaga
      241 tagataaact aaatgtatct tatatttaca ttccatatag ttaatataaa actatctaat
      301 cgacgaacag gtttatcgcc attttctata tttacattcc atatagttaa tataaaacga
      361 tttgtaacat taaaaaagaa taaaactttc tcagatttac attccatata gttaatataa
      421 aacgatgtaa taaatgagta ttcagtgcat ttcaaaaatt atttacattc catatagtta
      481 atataaaacc acatattgtt aacagctcta tagcaatgat tgaaaattta cattccatat
      541 agttaatata aaacattatg agtatcggga aagatgttgt gagaggctta atttacattc
      601 catatagtta atataaaact gatgaaatac atcaaactta tacagatgca tatagattta
      661 cattccatat agttaatata aaacattgta tgatttattt ttatagcggt actcctggga
      721 gatttacatt ccatatagtt aatataaaac ttattccaag aaggacgagt tttagcattc
      781 caagaacgat ttacattcca tatagttaat ataaaaccat tatgctatag tttcctagct
      841 ctccgacacc tacatttaca ttccatatag ttaatataaa actacaccag gttataatga
      901 aaatggagca ttagggaaat ttacattcca tatagttaat ataaaacgag tctaaattac
      961 aagcagaagc ggaaaaaatg ataaatttac attccatata gttaatataa aacattgtat
     1021 gttgatatta ctattctttc agttaaaaga tttacattcc atatagttaa tataaaacgt
     1081 ggttggaact tataaaaaag atgctgataa gttatttaca ttccatatag ttaatataaa
     1141 acgttctgtt gcaacttttg aaatgtattg accaagtata tttacattcc atatagttaa
     1201 tataaaacga tttgttcctg ttagtctttt tactgctgta acgtatattt acattccata
     1261 tagttaatat aaaaccccaa aataaattaa gtatttccaa tacttataca tatacaattc
     1321 tcctaaactt gcagtgagcg agcaatagtg caattgataa cagttatcat acactctcaa
     1381 cgctttatat ttcaactatt aaaccctact ttatcacaaa aatcgctcac tgcaaaactt
     1441 ttatattttt attatatcat aaaactcata ctataaaata aaaatgggat aattttacaa
     1501 actaaaagca tctacatttt tataagtatt tattatattc tctattccaa tgattccttt
     1561 ttctttttat tgcatccttc ctatgtaatc gcaccttagc tcaataagtc ttttattttc
     1621 attaacaatt ttttaaatta ttcaaaatca tcaaattttt catcttatac agttaaatca
     1681 aaatatgtgt tatatctctt tatctatata cttttcttcc ttccatttct tttcctcttt
     1741 atacattttc actttgttac tttacaatct caaggttatt atctgctatt aaagttttat
     1801 ctttccaggt ttcacaaaaa taaattgaat aagtgcaaaa ataacagata caaaaccata
     1861 actattttct tttctataaa aaaatatata atcaacaaga aatataataa atgaaaataa
     1921 ctatgaataa atactctcat ttagaagaaa tagacaaaga tattccttaa aatttaagca
     1981 aaatattgtt taaataaaaa aactgactgt cagttaaatg ccagtcagct attttatata
     2041 ttttatccaa acatcactat atttgaacat tttaataatt aataatactt attaatatac
     2101 aaactttact catacacaga atatcttttc caaatttgct ctaaatcatc aaaatgctgt
     2161 ttaatatctt ctttttcctt catcccaaca gatatagcaa gagcatttga aatactaaga
     2221 gctggaacta gagaatctac aaatgaagcc atattactct taactagaag agtgttatct
     2281 gcaagtgatg ctactggcgc aaataagcta tctgttaatg atattacatg ggctcctttt
     2341 tctttagcat aattgacaat ttgataagat ttcttagaat aacgagggaa actaatagca
     2401 actattacat cttcttcatt tattcttaca atctgctcaa aagcatctcc catatccatt
     2461 ctaattatat gaacattgtc aagtattata tctaagtaaa aacctaaata ttgagctact
     2521 acaaaagaac tcctcattcc aagtatatat atttttctcg ctcttaaaag tttgttagaa
     2581 gcttctttaa acgctctctc gtcaatttct tctagtgtag accttatatt atctatatca
     2641 ctttttaaaa ctttgtttag tattgcaaaa tcatcggaat aatcatgtgc catttcaact
     2701 ctttgtactg tagttagttt attttttatg agttcttgaa gtgcagcttg taatttagga
     2761 tatccactat aacctaatgc atttgcaaat cttacaactg tagactcact tactcctaca
     2821 gtttcaccca atttacatgc agtcataaat gcaactttat cataattatt taaaatgtat
     2881 tgtgctataa gtttttgacc cttactcaat ctcgtatatt gagattgtat atttgatatc
     2941 aaatgtttac tgtccttcat ttcatttgtt tcttccattt ttttctcctt ttagttatat
     3001 aaactatttt gttttattaa cactgtcttt ttctctaaaa attttatttt ttatctcaat
     3061 ttcatcttag tttttatcat agcttcttaa actcataaaa ttctaacttt tctgtacctt
     3121 atacttcaca tagtattttc gattaaattt attataccat ctttccttgc attttttaat
     3181 atttatccaa tatttctttt aaatttttca atttttactc atttaaaagt tatctatctt
     3241 attttgcata tttagatgta tattttgttt tgaaaataag ctgttgtata cttattattt
     3301 aaagtatttt taataggtca cactatatat attaactctt aatacaatct tgtcaagttt
     3361 tttagtattt attatgcttt tttcttttca attaaaatca attgtggtgg gttattgact
     3421 tgatttaaaa acttagattc taaaacatta aattcatctt gatttaaatt attgacaagg
     3481 ttatatatgc aatctttttc ttcttgacct ccatcatgcc ctgtatatat ggcaatgctt
     3541 attactccat tttcttttaa catttctaaa cttaacttta tagcctttaa agttgtatct
     3601 ggtttggtta taattgaatg tttcgctctt ggaagataac ccaaattaaa taaaacacat
     3661 gaaacttctt cttttatata ttgattcatt ttttcatgcc catcaaatat caattttact
     3721 ctatctgtat atccttcttt ctctagtttc tttcttgtag actttattgc ttcttcttgt
     3781 atatcaaaag catatacaaa gcccttttct ccaaccttct cagctagata ccttgtatca
     3841 tacccatttc ccatagtagc atcaattact atgtcacctt catttataat attttctaaa
     3901 taaaatttat ttatatctgt aattttagtt aaatactttg catatgccat aatactattt
     3961 ctccctacta ttaactttat ttcatttttt ctcaatttat ttttatctta ttttattgat
     4021 tttaaatatt ttatttcatc ttctgttagg tatctatatt ctccaacttt ggtgtctttt
     4081 aatactattt tccccacaga aatccttctt aaatttaaaa ctggatgatt tatagctttg
     4141 cacatttttc ttacttgtct atttctaccc tcatgtatag ttatttcaca cactgaataa
     4201 tttttttctt tattttcttt agttaccctt attttggcag gtgccgttgt gtaatcttct
     4261 atttttagtc ctgtttcgaa ttgtcttatc tcatcatcac ttataattcc cttcacactt
     4321 gccacatacg ttttatcaat ttcatgtttt ggatgggtaa gcttatatgt taaatcgcca
     4381 tcattagtaa gtattaataa tccactagtt tcataatcta atctccctat tggatatact
     4441 ctttccctta catctttaag tatgtctatt acactgggtc tattgaattg gtcttttact
     4501 gtagttatat atccctctgg tttatttaat actatatata tataattttc acttggtttt
     4561 acttttttat tatcaaattc tactatgtct ttttcttcat caatattaaa agataactct
     4621 tttactacac ttccatttac ctttattctt cctccaagaa ttatttcttc tgcttttctt
     4681 cttgatgcta ttccacaaga agctatatac ttattaattc tcatacttaa tccttcctta
     4741 tcttaaatta tgtgtgttta atatttttat caatgttaat tttgctacat tattatacta
     4801 tactagacct taattaaaaa caagcaaaaa aattatttaa ttttcctaaa taattgtaat
     4861 ttcttttcac atactattaa tatgtaatag ctgactaaac actattttaa ggaagtgatt
     4921 attatttcaa aggaaaatat aaaatattat ttaatagtcg tgtttattgg aattttattt
     4981 tttaaattta ttaatactcc atcagacttt atatctagta ttgaaggatt cttaaagttt
     5041 tttagcccat ttttattagc tattttactt gctttacttt taaatccact tgtgatgctt
     5101 tttgaggtaa aattcaaagc acatagactt ttagccattt ttttatctta tatatttata
     5161 ggttttattt tagcctttgc cattaggtta ttaattccat caatagctaa tacattaaat
     5221 agattaataa atgaaatgcc tatgtatact gattatatag atagttttat agaaaaaaat
     5281 atgtcaaaca tagattttct aaaaacttta attcctcata tacaacatag tttggataat
     5341 ttattaaaag aagcaagtaa ttttgttggt aaaattccta aaaacttctt aatctataca
     5401 cttagtatat cttctatgct atttaatatg acaatgggat ttatactatc catatacatt
     5461 atatatgaca aagaaaaaat tgcattggga tttaagagat ttctatattc ttcaactgct
     5521 agaaacaagg cagataacat tatagaattt tttaggacta ctcatgatat tttctatgat
     5581 tatttacttg gtagaatatt agactctctt attattggta taattgcatt tttaggattt
     5641 caatttgtta tcagaataga aaatgcttta tttttagctt caataatatt cttaggtaat
     5701 ataattccct attttggtcc ttttattggt gcaatccctc caatagccat gactatatta
     5761 tatagtccac aaaaaactat atgggtcatt gcatttctat ttatattgca acaattagat
     5821 ggaaatttta tagaaccaaa agttatgggt aatcaagttg gtataggagc tatatgggta
     5881 atatctgcta ttttggtggg acaatctcta tttggtttca taggagtttt tctttcagta
     5941 ccaatagctg ctgttgtaaa aacttatgtt gataaatata tagataatcg tttacaatag
     6001 attatctcta ctgtgacttt aatttttagt ttaaaattta actgctattt attaaattat
     6061 ttctcaaaaa atatctctta gtaagaaaaa attgctaaga gatatttttt ataatatata
     6121 atttgttcat taaatttatg catatatttt atacttattg attatttgag ttttgagaat
     6181 ttccaccact atctgaacca ccagtagtat cttgtggagt tggtgctggt gttggattac
     6241 tattatttgg cttttcttga ttttgagaag aactgttatt attgtcagac ttattactat
     6301 ctgatttttt atcattgttt ttagaactag atttattatt tttactactt ccactataag
     6361 aagatttcct tgcatctgat tcaaccctat acctatcttc tccagccgta tcttttgcct
     6421 ttttctttct ttgttcctct gcttcgtttt gttctttttc taatttagtt aactcttctt
     6481 tctttttagt tgcctcttct ttaataacag aggaaccatt cttgattttc tctataagtt
     6541 taagttcctt gattgcaccc tcataatttt ttaattcttc aaactccgtc atatttgaca
     6601 ttctcatagc ttgcatatac atagctcttg cattctcatt agtactatct tcttccagtg
     6661 cagaggataa tgattccata gcttttttat agttatgttt ttctaaatat gtttttcctt
     6721 gctctactaa atttatttca tcttttttat taaatgaaca tcctacaaga aatatagata
     6781 atatcgatat taataaaatt actcttcctc tcattaaatc cctccttgtc atatacttta
     6841 aattttatca taattataaa gtttttaaaa tattttatat ttattacaag aaatttgtta
     6901 gtaaaacaaa aaataagcag atatgctacg aacgatacaa taaaccaaaa attaatttag
     6961 agatacatat cctagcaaat ctgc
//
LOCUS       DAFKMX010000189         6944 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFKMX010000189 DAFKMX010000000
VERSION     DAFKMX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.192"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            9..389
                     /locus_tag="LZB08_003190"
     CDS             9..389
                     /locus_tag="LZB08_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7316657.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMSYGRMFSFKDQDGNDYLVREDK"
     gene            514..714
                     /locus_tag="LZB08_003191"
     CDS             514..714
                     /locus_tag="LZB08_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316658.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            910..1638
                     /locus_tag="LZB08_003192"
     CDS             910..1638
                     /locus_tag="LZB08_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7316659.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1660..2577
                     /locus_tag="LZB08_003193"
     CDS             1660..2577
                     /locus_tag="LZB08_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBM7316660.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2719..3903
                     /locus_tag="LZB08_003194"
     CDS             2719..3903
                     /locus_tag="LZB08_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBM7316661.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4022..4210
                     /locus_tag="LZB08_003195"
     CDS             4022..4210
                     /locus_tag="LZB08_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBM7316662.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4359..5009
                     /locus_tag="LZB08_003196"
     CDS             4359..5009
                     /locus_tag="LZB08_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBM7316663.1"
                     /translation="MKKNYEIILFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLDNLE
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            5112..6050
                     /locus_tag="LZB08_003197"
     CDS             5112..6050
                     /locus_tag="LZB08_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBM7316664.1"
                     /translation="MKSILIMGGSDFIGFALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6076..6927
                     /locus_tag="LZB08_003198"
     CDS             6076..6927
                     /locus_tag="LZB08_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBM7316665.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         2862 a          663 c         1257 g         2162 t
ORIGIN      
        1 gtattgttat gataaataaa ataggaaaaa taactctcta tgtaaataat caagaggatg
       61 caaagttatt ttggacagaa aaattgaact ttgtagtaac ttatgaaaag gaaatgggtc
      121 caaatatgaa atgggtggaa gtagcaccaa gtaaaaatga atttacaaca tttgtgttgt
      181 atgataaaaa tctaatgatg aagcaaaaca aagagataaa tgtatctcat ccatcaataa
      241 ttttaagtac aagagatatt aatggaactt atgaacaaat ggcaaataac gaggttgagg
      301 ttggggaatt aatggagatg tcatatggaa ggatgttttc ttttaaagac caagatggaa
      361 atgattattt ggtcagagag gataaataat tttacaaagt attaaattta ctcaatatct
      421 gatataatta gagacaataa ttattataaa ctagtctagg attagaatta tataattaaa
      481 aattaagtgt aaattttgag tagaggtgat aaaatgttta agaaaatggc agtactaaaa
      541 gatatagcaa ctaaaatagg tcgtaaaaaa gcatatgaac tattagaaat ggttgaaggt
      601 aatgatgcct ttgtagctga ggtaaagata aaaaagaatg gaatagaatc taaaaaagaa
      661 gaaattatgt taaaagataa tcaaaaaata atattagagt atatagaagg ttaaatttta
      721 aatacatata agttgatata ctaaatttct gagatgagtt tacataaaac tcatcttttt
      781 tacttttcaa taactttgtt ttattattta atatacattt tatctcctta gagttgctga
      841 tatataaaaa ttactgatat aataaaagaa tggatattaa tgtaatattt tatagaaaag
      901 aggggtatta tgcttgaaat aaaaaatctt tcctttaatg ttgagtctaa caatgaggaa
      961 ctaggaataa taaatgatgt tagcctttca tttgagaggg gaaaacttat tgttatcaca
     1021 ggtccaaatg gtggaggaaa atctacaatt gctaagctta ttatgggaat tgaaaaagct
     1081 acgagtggtc agataatttt agatggagaa gatataacaa atcttagtat tacagaaaga
     1141 gcaaaaaaag gaataggata cgcatttcaa caaccaccaa gaataaaagg tatgacagtt
     1201 gaaaatttac ttacacttgc tcatggaaaa ccattgtcta ctgatgtatg ttgccaatac
     1261 cttacagatg ttggattgtg ttcaaaagac tatttaaatc gtgaagttga taactctctt
     1321 tctggagggg aaatgaaaag aattgagata gcaactcttt ttgctagaga tttaaaggtt
     1381 tctatatttg atgaacctga agcagggata gacctttgga gctttggaaa actcaatgaa
     1441 agttttaaga aaatacatga agagtctaat cagactataa tcataatttc tcaccaagaa
     1501 agaatattag agttagctga tgaaataatt gttctacaag atggttcagt aaaaagccat
     1561 ggaacaaaag aggctatatt acctgaaatc atgtgtcaag ttaattcaag ttgtgaatta
     1621 atgaaagaca tgaattaatt agaaaggaag gcaaattaaa tgaacaaaat agatttaaat
     1681 cttttagaaa aagttgctga acttcgtgga actccattag gagcatacaa tatacgtaaa
     1741 aatgggcaag gagtagagag acattcaact gaaaatgtaa ctataattcc taaaactgat
     1801 aaacctggaa tagatattat agtaaagcca aatacaaaag gagaaaatgt acatatacca
     1861 gtaatagtaa cagaggctgg tgtacaggat atggtatata atgattttga aattggagaa
     1921 aatgcagatg ttgttataat tgctgggtgt ggtatacata actgtggttg tgatgatagt
     1981 gaacataatg gtatacatag atttttctta cgtaaaggag ctcgcctaaa atatgtagaa
     2041 aaacattatg gtgaaggtga tggagaagga aagagaattt taaatccagt tactattgtc
     2101 aatatggatg aggattcata ttgtgaaatg gaaacaattc aaattaaggg tgtagattca
     2161 actaaaagag aaatggatgc aaacctaggt aaaggagcta aaatagttgt atttgagaaa
     2221 ttattaactc atggtaatca agttgcagaa tctaatatga aggttatctt agagggtgaa
     2281 aactcttctg ctcaagtagt ttctcgtaca gttgggcaag ataattctaa gcaaaagttt
     2341 aatccttgtg ttattgggga ttccctttgt tctgcacatg ttcaatgtga ttcaataatt
     2401 atgggaaatt ctcaaattag tgctatacct gaaattgctg caaatcatcc agatgctctt
     2461 ttagtacatg aagcagccat tggaaagatt gcaggagacc aaatactaaa gatgatgaca
     2521 ttaggtctta ctgaagaaga agccgagcaa caaatactta attgcttctt aaagtaaatt
     2581 aaagatttta tttaatttaa tataattaat atagtataat atagtgaata agtctaaaat
     2641 atttttattt ttaggcttat tttctttatg taagaaaagc tattagagaa attatattgt
     2701 aattaggatg tggaataaat gaaaaattta cttataagaa atgggacaat aatatctcca
     2761 acagatggta tcaaatttga aaaaagagat atcttggttt tacatgggaa aatatatgct
     2821 attggattga accttaaaga gaaaataaga aaagaaaaat tacttacaaa taaagagttc
     2881 gagataataa atgccgataa tatgtatatt tccccaggat ttatagatgt tcacacccat
     2941 tgttataaga gaaaattacc tacaggtatg gattctgatt caattggaat tgaaaaaggt
     3001 tctacagtta tatttgatgc aggtacagct ggtccttcaa actactatga ttttaaaaaa
     3061 aagtatatgg atgagtgtaa aacagaagta tattcttttt taaatgttac aaatgaggga
     3121 ctaaatatat tgaatgaatc aacttcttta gatgttataa attttgacaa ggttgagtca
     3181 attgtagaaa aaaatataga aaagataaag ggtataaaag taaaagcgag taaatttcaa
     3241 tctaatgaaa gtggtataga tataattaag aaatcaaagc aagttgctaa aaaattaaat
     3301 ttacccttag tggtatatat aggagagtat ccatcatata tagatgaagt aatgaatatt
     3361 ttgggaaaag gagatgtggt tacaccggta tatggaaata gtacaaatgg aatccttaaa
     3421 gaatctggca cattaagaaa atcagtaata aatgcaaaga atagaggagt attgtttgat
     3481 gtatgtcatg gagtggacca atttgatttt aaagttttta aaaaagcaat aaagcaaggt
     3541 ttagagcctg acctaataag tactgatatg catataaaaa acatgaaaga cgtaaactat
     3601 tctcttttaa atgttataaa taaaattatg gaacttggga tatccctaga aaagtgtata
     3661 gaaaaagtaa ctgaataccc aacaaagatt ttcgatttac atggaaataa aggcagaata
     3721 aagattggtg gagaaggtga ttttacaata ttcacaatgg aggagtgtaa tgatattata
     3781 aaagactaca ataataatga acttgtttta aataaaaaat taagattgca atatactgta
     3841 aaatcatgga tgaaaagcag tgaagtttat agacatggat acgaaaatac tataataaac
     3901 taaacaatag tgataaaata ataaatatat gtttaaattg gtatatattg gataaatatc
     3961 taaggaaaca acaattgaaa caacaattaa aaaataataa ttttagggga ggtattttag
     4021 tatgaaagca tttgttgata aggatgtatg tgtagcttgt ggagcatgta taggaacatg
     4081 tccagacgtt tttagtatga gcgatgaagg gcattcagta gcaattgaag gagaaatacc
     4141 agcagattta caagatttat gtaaagaagc cgaagaagga tgtccagttt cagctataac
     4201 agttaaatag agctgtttaa atttattttt aaaataaata tataagattt aattggtact
     4261 taagctatag tattagaaac ttctattact atagcttttt aaaatagata aacttaatat
     4321 ataagatatt actttttata aatatggagg actgaaatat gaagaaaaat tatgaaataa
     4381 tattgtttga tttagatgga acacttacag accctaaaga aggaataaca aagtctatcc
     4441 agtattcttt aaatagcttt ggcatagaag aagatttgga caacttagaa caatttatag
     4501 gaccgccatt acatgatact tttaaggaat attataaatt tgaggataaa aaagctaaag
     4561 aagctgttga aaagtataga gaatattttg ctgataaagg gatatttgaa aataaaattt
     4621 atgagaatat gaaagagata ttagaaatgc tctataaaaa tggtaaaata ttattagtag
     4681 caacttctaa gccaacagtt tttgcagaaa ctatacttag atattttgat atagatagat
     4741 attttaaata tatagcaggt agcaatcttg atggtacaag agtgaataaa aatgaagtta
     4801 tacaatatgt actagaccta tgtaatgtaa aagataaaga taaagttatt atggttggag
     4861 atagaaagta tgacataatt ggagctaaaa agataggtat agattctatt ggagtattgt
     4921 atgggtatgg ttcttttgaa gagcttagtg aaagtgaacc tacttatatt gtcgaaaatg
     4981 tagagtctat aaaagatatt ttgttataaa tatcttttat agacaaaaat attacaaaat
     5041 ggtgtcgcat atttaaaaat atgatattat agttaaggaa gtcatttaaa aaagaggagg
     5101 atgcacttaa aatgaaatct attttaatta tgggtggaag cgacttcata ggttttgcac
     5161 ttgctaagcg cttgattaaa tgtgggtatc aaattgatat acttacaaat ggtaaaaagg
     5221 aaatagatta taatggtttt aaagaacatc taatttgtga tagaaaagtt cgaaaagata
     5281 tggaaaacat tatcacagga agaaaatatg attacatata tgatatgaca gcatatacaa
     5341 aagaagatgt aagtaatctg attgatttta taagcatgga taacttaaaa aagtatatag
     5401 tattgtcagc aggagcagta tataaagata gtggaagaaa tataaaagaa gaaaatgaga
     5461 aaggtgaaaa tgaaaactgg ggaaaatatg ggttaaataa aaaagaggct gaagatttta
     5521 ttataaatag ccctatacca tatataatta ttagaccaac ttacatatat ggtgaaaata
     5581 ataatttata tagagagtat tatttctttg aaaaaataga aaaaaatgaa aagataccag
     5641 taccaaaagg aaaacaagtt agtaatcaat ttatatatat tggggatttg gtaaaggtat
     5701 tggaaagcat aatgaaaaat cctcatgtaa gagaggccta taatgtgaca aatccacagt
     5761 taatttcttg ggatgacctt atatatacat gtggagaaat tattggcaaa gaacctataa
     5821 taaaatatgt agatatggaa aaagtagagt ttagagaaag aacgtatttc ccatttagaa
     5881 atatagactt taatcttgat ataaataagc ttattgaaca tgggctttat ataccaaacg
     5941 tgttactaaa agaaggtctt acagcaacat ataaatggtt ttcagcaaat aagccaaaaa
     6001 tgcatgacag aaaaatgaac aaagttgagt ctgtaatgca aatagtttaa aaaaataaat
     6061 aagtaggtag atattttgaa ctcaaaaatt aagtattttt taaaattttt tatcttaata
     6121 tttattttga tttttatata ctcagttgcg atagagccga gtctattaat tgtgaaaaaa
     6181 tataatataa gtgaaaatgg agcattgata aaaaatgata caattgagag taaatctgaa
     6241 agtgtaaaag tagttcaaat ttcagatact caaataggta gtttttattc aactaagaat
     6301 ctcaaaaaag tagctaataa gattaacact ttgaatccag atataatagt atttactggt
     6361 gatttaattg attactccaa taaaaatcca agtgtagatg aaattacaac tattttatca
     6421 agtatgaatg ctaggttggg aaaattttca gtatttggca atcatgatta tatgtataaa
     6481 cttcctaggt attataggca gattatgaaa aattccaact tcaatctttt agtaaatgaa
     6541 aataaaaaaa taaaattaaa agatgataaa tatattaata tattaggtgt tgatgaaata
     6601 ttaaatggaa atcctaatat aaaatatctg gagagtcaaa tagataataa aagttttaat
     6661 ttattgttag cacatgaacc agatttagta gatatgctta gtaaagatac aatgaattta
     6721 gttctatctg ggcatagtca tggtggtcaa ataagattac ctattaaggg agcattagtt
     6781 actcctccat atggaaggaa atatactaaa gggttttatg atataaatgg aaatcattta
     6841 tatgtgagtt cgggtcttgg aagtacaaag ttaccattta gattttttaa tataccagag
     6901 ataatagaat ttaatattat aatctaacat aaaatattta gaaa
//
LOCUS       DAFKMX010000190         6931 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFKMX010000190 DAFKMX010000000
VERSION     DAFKMX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.193"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            22..2199
                     /locus_tag="LZB08_003199"
     CDS             22..2199
                     /locus_tag="LZB08_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBM7316666.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2213..2635
                     /locus_tag="LZB08_003200"
     CDS             2213..2635
                     /locus_tag="LZB08_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7316667.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2809..3384
                     /locus_tag="LZB08_003201"
     CDS             2809..3384
                     /locus_tag="LZB08_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7316668.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNNYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3509..4225)
                     /locus_tag="LZB08_003202"
     CDS             complement(3509..4225)
                     /locus_tag="LZB08_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBM7316669.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4390..5115
                     /locus_tag="LZB08_003203"
     CDS             4390..5115
                     /locus_tag="LZB08_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7316670.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5175..6767
                     /gene="hcp"
                     /locus_tag="LZB08_003204"
                     /gene_synonym="priS"
     CDS             5175..6767
                     /gene="hcp"
                     /locus_tag="LZB08_003204"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBM7316671.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMILSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
BASE COUNT         2707 a          785 c         1245 g         2194 t
ORIGIN      
        1 tatgatattt gaataaaaag aatgtataat aagtataata atttgtattt attattggga
       61 aaggaaaatg ttaatatgga tagttataga caacttaaag ctaaaatacc ctgtaaggaa
      121 acaggtatag aaataaagag gactttatgt gatatttgtt ctccaggagt acattgtgga
      181 attgatgcat atgttaaaga tggtaaagta ataaaaatag aaggtacaaa agaacatcca
      241 atgaataaag ggttgttatg taccaaaggt ctttgcaata gagaatatat ctatagagaa
      301 gatagaataa aaacacctct aaaaagaatt ggaaaaagag gtgaaggaaa atttaaacca
      361 atatcatggg atgaagctta tagagaaata gcagaaaaat taaatagagt aaaatctgag
      421 tatggagcaa agcatgtagc tttttttaca ggttatggaa agtggtatag acctttttta
      481 caaaggtttg catcttcatt tggttcacct aattatggta cagaatctag ttcatgttca
      541 acatcagcac agatggctgg aaatgttgct actggaagaa actttacaat gcaagatatg
      601 gagaatgcaa aaacttattt agggtgggga tttaatccat actactctaa atttttaaat
      661 agtgtgaatc taataaaatt aaaagaaaaa ggtttaaaat ttataattgt agaccctaga
      721 ataacaccga caactcaaaa actagcagat ttacatttaa gaattaaacc tggaactgat
      781 ggagctttag ctcttggaat ggcgaatcta tttatacaaa aggatttaat agatagagaa
      841 tacatagaaa agtatgtaca tggatttaat gaatataaag aatatgtaaa attatttgat
      901 ttagatactg taagtagaat tacaggagtt tctaaagata atatagtaaa agctgtcgag
      961 atgttgtcac aaggtccatt ttgtataaat gaaagtgcag ctccaatagt tcatcatcaa
     1021 aatggatttc aaaactatag agcaatgatg tctttatccg ctataacagg taattatgat
     1081 agaataggtg gaaacatacc aaaacaagat tactttgctg gtaagatagg tggattccaa
     1141 acactagaaa aagagtttgt aaaagaagtt ctacctaaaa atataaatga aagaattggt
     1201 ggagaaagat ttcctctatg gaatgaaatg gtgactcaat ttcaagctat ggatttacct
     1261 aggcaaatag aagaaaagac accatatgaa ataagagctt tgttttgtca tgggataaat
     1321 gctagaatgt ttcctaattc agggagactt tttaaagcgt tgaatagtgt agattttttt
     1381 gtgaatatag atttatttat gacagataca tcaaaatatg cagatatact tttacctgca
     1441 tgtacatcat ttgagagaga agaatgtaag agttatccag gagggtatat tacatatacc
     1501 aaaaaagtta ttgataaatt atacgattca aaatcagatg tagaaatact ttcagattta
     1561 gcaaatgcta tgaatataga tgatgattta ttaaaagcag gttatgaagc aagtgtaaga
     1621 catatgttta aaaatacatg tgtagatgta gataaactta aagaaagtga tttaccatta
     1681 aaatgtaaag attttaagcc atatatagta ggttcatata caagagaagg gtatgatact
     1741 agcacttcta ggtttgaatt aaaatctaca attattgaaa aatataaaga ttttgggctg
     1801 gatgcactac caacatatag aggatttaat gataatatag atgatgaata tactcattta
     1861 ttgacatcag gagcaagaat ttcaaatgca ctacattcaa gacttcatga catcaaactt
     1921 tcaagaatat taaaaaatga agctagttgt gatatttcta gcttagatgc aaaagaattg
     1981 ggtgttatca gtggagatta tataaaaata actactaaga taggaagttt ggatgtaaaa
     2041 gtcaatgtta ctgatagagt cttatataag aatatacata tgtatcatgg atatagagaa
     2101 gctgatataa atagtattat ttgtgataat tttgaccctt atagtggatt ccctgcgtat
     2161 agaagttgta agtgtaagat tgaaaaaatg aatagataaa tggagagata taatgaaaag
     2221 tttatttaaa tttaataaag aattatgtac agcatgtgga gcatgtttgc ttgcatgtat
     2281 agaccaaaat gataataata tagacaatgg tgaacttttt agacatgtat ttacaataga
     2341 ggaaggtgaa aatatagaat atttttcatt agcatgtata cattgtaatg agccaaaatg
     2401 tttaggtgta tgtcctaaaa attgttttaa aaaagaagaa ggattcgttg tattagacaa
     2461 tcaaaactgt atagggtgta aactttgtga aaaagcttgt gaatatggag ccttaattta
     2521 tggaaatgat ggtaaggcaa ataagtgtaa tggttgtatt gagagatata agtgtggtat
     2581 ggattcacct tgtgtaatgg tatgtcctac aggagcatta aaatttaata aataattgta
     2641 ttaaattgct atactacttt taaataattg tgttaagaat tattttttgc tatacttttg
     2701 taatataaaa tgtaaataaa acaaatacaa agtatataaa atatattttt agaataaagt
     2761 gtgctttaga ttaaatttag aaaaataaat tagacaggag aacttaatat ggtaaaagta
     2821 ttagccttta tgggcagtcc aagaaaaaaa gggaatgtag acacaatact tgatgagata
     2881 ataaaaggtg taaatgataa taatgttgaa gttaaaaaat attatctaaa tgatatgaat
     2941 attaaaggat gtcaaggctg tctatactgt agaaaagttc ctacttgtgc atttaaagac
     3001 gatatgattg acatatatga agatataaaa agtgctgaat atataataat tggttcgcca
     3061 gtatatatat gtcaggtatc agctcaaaca aagctacttt tagatagatt gtatccttta
     3121 acagagataa ataagtctaa acatatacct aggtttggtc aaaaaaagtt aatcatggtc
     3181 tatactcaag cagctcctat ttcgttttta tttaaaaaat attttaagta tactgcaaaa
     3241 catttaaagg gcatgggatt aaataactac aaaactatag ttgtaaccaa agcatttgaa
     3301 agaaattcca ctaaaaaaaa ttttaaggta ttgaaaaaag cctataaact tggaatgaaa
     3361 atagccattg atatcaatgg atagataaaa gtttaattta tagtatagct aaaggagcat
     3421 taattattaa tactcctttt ttgtataaag gtttctagaa aataactaat aaaaaacaat
     3481 attatttgaa tacagatatt atacaagcct agatatctaa cttgttttga aaataaaaga
     3541 tttcaaattt atttttgtta aaagataaca agccttcatc tcgcatcttg ccaagctctc
     3601 ttgacataga acttctgtca acacataaaa aatcagccat ttcatttctt gaaaagggaa
     3661 ttgtaaactg gtggctacct gtttgttcta tctgttttga aaaatacata agcaatttct
     3721 cgcgagttgt tttacgagaa agtaattcta ttttattgtg aagtaaaatg tttttttcag
     3781 ctattaattg tagcatattt tcaacaagcc tattatgaaa tacacatgca gatgaacaaa
     3841 taccagttac acttttaaac tttataaata aaacttcgca agaagatgtt gtggttactg
     3901 taacattact tttatgtagt ctagtacatg cgaatacctc tccaaataaa tctccaggca
     3961 agagttcagc tacaatattc ctattccctt cgatatcttc ctttattata tgagcacttc
     4021 ctttaagcat aattccaata gaagtaactt cacttccaac agagaatatt atactatctt
     4081 ttgggtaagt cttatctaca acacctaaac attccaacat ttcttcaagt tcatcacttt
     4141 taattccttc aaatagagga atttttttaa gtaggtctat atgcttttct aaaaattttt
     4201 tcaaataatc acttccttgt tgcatatgca accgataatt tgttttcatt gtactatact
     4261 aagtttataa aagcaagggg atataaaaat cctactacaa gaaatttgaa ataaagaaat
     4321 ataggtatta aaaataaata tataagaaat caatacttta aattaaaaaa attttatgag
     4381 gtgatataaa tgatgagaaa aataattaaa attgatgagg aaaagtgtaa tggttgtgga
     4441 ctatgtgttg aagcttgtca tgaagaagct ataggaatgg taaatggaaa agcaaaactt
     4501 ttaagagatg actattgtga tgggctggga gattgtctgc caacttgtcc aacaaatgcg
     4561 atttcatttg agtatagaga agcagcagaa tatgatgaag ctgcagtaaa agcaaatatg
     4621 gaagcaaaga aagcacaaaa gaaaacttta gcatgtggat gtccaggttc tcagtctaag
     4681 tcaataaata gagaggtttc aaatagtacg tctataagca atgatatagt agagataaaa
     4741 ggttctcaac ttaatcaatg gccagtacaa ataaaattag taccaactaa tgcatcatat
     4801 ttaaagaatg caagtctttt aatagcagca gactgtacag cgtatgcata tggaaatttt
     4861 cataataaat ttatgaaaaa taaggttaca ttaatcggat gccctaaact tgatgaagtt
     4921 gactatgctg aaaaacttac agagatttta aaagaaaatg atattaaaaa tatagtagta
     4981 acacgtatgg aagttccttg ctgtggtggt atagtaaatg ctgttaagac agctctacaa
     5041 aatagtggaa aaatgatacc atggcaaata gttactatat ctgtagatgg aaaaattgta
     5101 gatggagaaa tttaattaag tgaattatag aaaaatataa taaattttaa taaataaaaa
     5161 gggaggaata aaaaatggaa aaaatgtttt gttttcaatg tgaacaaaca gcaggaggta
     5221 aaggttgtac tataaatggt gtctgtggta aaaagggggg tacagctaac ttacaagacg
     5281 aacttacagg agtgttgatt ggtctttcaa gagcaactat tggaaataag aatagaccaa
     5341 caagtgaaac agataaaatt atgatagagg ggctattcac tactataacg aatgtaagtt
     5401 ttgatgatga agctataaaa agacaaattg aaaaaactga ggtagaaaaa agtaagcttg
     5461 tacctagatg ttcagattgt tcaacaactt gtaatagaaa tgataactat gatatgaagg
     5521 atttatggaa tgataatgag gacatacgtt cattaaaatc attaatctta tttggattaa
     5581 gaggtatggc tgcctatgca tatcatgcaa tggtacttgg ttatacagac aaagacgtaa
     5641 atgaattttt ctatgaagga ttaatagcag ttggaggaga cttgtctatt gacgaacttt
     5701 taggtctagt tatgaaaact ggagaaataa acttaaaatg tatggaattg cttgatagag
     5761 caaatactga aacttatgga acaccagaac cgactcaggt atctatgaaa atcgagaaag
     5821 ggccatttat tgtagttaca ggacatgacc tttatgattt acacaaatta ttagaacaaa
     5881 cgaaagataa gggtataaat atatatactc atggtgagtt attaccagca catgcatatc
     5941 caaaactaaa agaatataaa catctaaaag gtaattttgg tacagcatgg caaaaccagc
     6001 aaaaagaatt tgataatatt cctgctccaa tattatttac aacaaactgt ttaatgcctg
     6061 ttaaagatag ttatagagat agagtattta ctacagaagt agttgcttat ccaggtatgg
     6121 ttcatattga tgaagataaa gatttttcta gtgtaataga aaaagctctt gaacttggag
     6181 gatatagaga agatgttcat atgactggta ttaatggagg agatacatta actactggtt
     6241 ttgcaagagg tacagtttca tctgtacaag ataaagtagt agatgctgta aagagtggag
     6301 ctataagaca ctttttattg gttggaggtt gtgatggtgc aaaacctgga agaaattatt
     6361 acactgaatt tgtaaaacaa gctcctaaag acactgttat attgacactt gcatgtggaa
     6421 aatatagatt taatgattta gaccttgggg atataggtgg cttacctcgt ttaatggata
     6481 tgggacaatg taatgatgct tatagtgcaa ttaaagtggc agtttcattg gcaaatgctt
     6541 ttgaatgtgg agtaaatgat ttgcctctta gcatgatact gtcatggtat gagcagaaag
     6601 ctgtttgtat acttcttaca ctacttcatt taggtatcaa aaatatctat ataggacctt
     6661 ctttaccagc attcttatct aagaatgtat tagatatatt ggttgataaa tttgcattga
     6721 cacctatatc tactcctgaa gaagatatga agaaaatttt aggataaata aatatatcta
     6781 tacttaaaga tatttatagt taaactattt ttaagtatgc cacaattaag ataaggggtt
     6841 aaacttaaaa tttgattaaa aaatcaattt tgagatgacc ctttatttat tttttgtaat
     6901 aattgaaaaa taaacctgct ttcagtatat t
//
LOCUS       DAFKMX010000191         6857 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFKMX010000191 DAFKMX010000000
VERSION     DAFKMX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.194"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            364..1197
                     /locus_tag="LZB08_003205"
     CDS             364..1197
                     /locus_tag="LZB08_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBM7316672.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1290..2222
                     /locus_tag="LZB08_003206"
     CDS             1290..2222
                     /locus_tag="LZB08_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBM7316673.1"
                     /translation="MVGMLSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2274..2756
                     /locus_tag="LZB08_003207"
     CDS             2274..2756
                     /locus_tag="LZB08_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7316674.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2768..3637
                     /locus_tag="LZB08_003208"
     CDS             2768..3637
                     /locus_tag="LZB08_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBM7316675.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQDRMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3680..4435
                     /locus_tag="LZB08_003209"
     CDS             3680..4435
                     /locus_tag="LZB08_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBM7316676.1"
                     /translation="MTTYKKLKKSIFKIAIFLICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRSISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4477..5298
                     /locus_tag="LZB08_003210"
     CDS             4477..5298
                     /locus_tag="LZB08_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBM7316677.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5303..6064
                     /locus_tag="LZB08_003211"
     CDS             5303..6064
                     /locus_tag="LZB08_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBM7316678.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIVEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6225..6482
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6595..6834
                     /locus_tag="LZB08_003212"
     CDS             6595..6834
                     /locus_tag="LZB08_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBM7316679.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         3019 a          635 c         1154 g         2049 t
ORIGIN      
        1 aacaaaaatt agataatatg aatatataat gtatgtatta aaaatgtggc aaatagttgt
       61 tttgctattt attatttgtt taatatttct aatttataaa aaaaaaatta taaattttaa
      121 aaattataaa tgacatttta actagaatta attgtaaaaa acactatata atactcaatt
      181 ttcaacattc tatacaatta tacagaaatt atacagcata ataatcacca aaataacaaa
      241 ttttaaaaat taagcaaaac tttttgtcga agttcaacgt ctatatatat gtagtgtaaa
      301 tttttgggat aaaatactac aaaaatataa atgtaaaaat atagtaatat tggaggtcga
      361 acgatgagaa ataaaaagaa aaaaataaat agaaagaagt tgtatttgct tttaagtgct
      421 attgtagtgt gtctggcttt tatagtagtt ggtgttcgcg tcagctcttt aaatatgaag
      481 gaaaatgaaa ttagtagaaa tgcaaaactg tcaagttcta ctattacaga tatagtatct
      541 aatacatcaa tagaagttag taaagggcca agtaaaagtt ctggaaaaat agcatacata
      601 actatagatg atggtccatc taaatttact gacgagatga taaaaacttt aaataaatac
      661 aatgttaaag caactttttt tatgatagat gggaatatga aggaatatcc acagcaagtt
      721 aaaaatataa ttaaaaatgg gaacacagca ggttttcata gtgtatcaca tgatattcac
      781 aagctttatg ttactagtac atcagcaaaa gaggaatttg atacaaatga tcaaactttt
      841 tataagataa ctggtaaaca ttcaaaagta ataagaatac cttatggaag caaaccatat
      901 acaccacaag cctcttatca ggctttagtt gatgctggat ataaaatttg ggactgggac
      961 ttagatacag aagactggag atctaattct tctcaaattg tacaaaatgt aaaaaatcat
     1021 attaagaata gaaaaggtga agataaagac caacttgtag ttttaatgca tgagaaaaaa
     1081 cagagtgcag aagctttaga ttcagtttta aaattcttat cagatgaagg ttatgaattt
     1141 gcgccagtag atcaaaacca aataccaaaa aattattggt tacgtaattt agagtagtta
     1201 aaatataaga atattgattg atatttttag aaattgagaa taaaaatgag tgaggtgtag
     1261 gtatattgaa taaaaaactt tatataggaa tggtcggaat gttgtctttg atgatggttg
     1321 gttgtaataa aagtttagca aaagtaaatg atgtggaaat cacaaaagaa cagtacaaaa
     1381 aaacaaaagc agttttatct gctactaata attatataaa tgggcagtct ttagatgaat
     1441 tagaaaaaac tttggataaa aaaggtagaa ataaattaga aaatgttata atatcgttta
     1501 tggtggataa tgaactttta tatcaagagg ctaaggataa aggactaact ccaagtaaaa
     1561 gtgaagtaga ctcaaaatat caagaactag aagataagat gaatttgaat acaagctata
     1621 aggaaaaaat ggacaaagca ggtgttgata aagattattt aaaacaagaa atatctagag
     1681 atttagcaat agataaaaat aaaaaggcct ttgaagatag aataaacata agtgataatg
     1741 atatggaagc ctactataca agtcataaaa aagattttaa tgtagaagaa gtaagtgctt
     1801 ctcagatact tatatcgact ttagataaaa ataaaaaaga agtaagcaaa gataaaaaag
     1861 aagctttgaa gaagaaggca gataatatat tgactaaaat caaaaatgga gaaagttttg
     1921 aaagtttggc taaaaaatat tcggatgata aagcaactgg aaaaaatgga ggacagctag
     1981 gatattttac aaaggatgat aaaaatgctg agtttacaaa ggaagtgttc aaattaaaga
     2041 aaaatgaagt ttcaaatgtg tttgaaacta gttatggata tcatatagtc aaagttactg
     2101 ataaaagaga aagacaaaaa agttttaatg agtgtcagag tttaataaga gaatctattt
     2161 taaatgaaaa gtatattgag catataaaaa aactgaatga agatgctaaa atagacagat
     2221 agaatttcta acagctcaat cctagcctgt aatcggaggg agattttaca ggaatgttta
     2281 agagtttaaa gagaacgaaa gttgagaaat acattatcga taataaagac tctttttata
     2341 gaatagcata tagttatacg aagaatgaag aagatgcctt agatgtggtt caagaagcca
     2401 tgtataaggc actatactca gtggaaaata ttaaagaagt aaattatata aagacatggt
     2461 tttataaaat tttagttaga acgagtatag attttattag aaaaaaccgt aaatataata
     2521 atatgacaga tattgacctt atagatgaga ctggagagta tgataaatat actgatttgg
     2581 atttaagaag agctttggaa gaattaccta ttgaatacaa atctattata atattaagat
     2641 tttttgaaga tttaaagatt gaagaagtag caataatatt ggatgaaaat gtgaatactg
     2701 ttaaaactag gttatatact gctttaaaaa aactaaaatt aaaaattgaa gagtagggat
     2761 aaaaagaatg acaagcaaag aaaggttaaa taagttaaaa gatgagtatc ataaaattcc
     2821 aatacccaag aaattagata ctataataaa tcatgaaaaa atagaaaaca tatatcgaga
     2881 gaaaaataaa aaagtaaata gattaagatt taaagttgca atagcctttg catgtatatt
     2941 tacagtatta gtaaatataa gtcctgtatt tgcagacaat ttttctaaga tacctgtaat
     3001 tggagcaata gtagaagtaa taactataaa aaattatagt ttaaaaagtg aaaattatga
     3061 agctgagatt gatattccta aaatacgagg tttaaaagat aaaaacttag aacaaagatt
     3121 aaacagtagt tttatggaag atggaaaaag gctttatcat caattccaag atagaatgga
     3181 gaagatacaa tcatcaaaga ataaaggata taaatcttta agtttgagtt atagtgtaaa
     3241 aaataattct aaaaaatttc tatcaataga gatgacaaaa aatgagatag aggcttcatc
     3301 atatgtgagt aaggtacatt atactattga taaaaaaaga caaatagttt tgactttgcc
     3361 aatgcttttt aaagatgata agtatataaa agttataagt gataatataa aagaacaaat
     3421 gagagaacaa atgaaaaagg atagtacaaa atcctatttt atagatcaaa aaaaagactt
     3481 gcctgttgaa gattttaaaa caattaataa gtatcaggat ttttatttta ataaaaatga
     3541 agaccttgta atttgttttg atgaatatga agtagcacct ggttatatgg gcgcagttga
     3601 gtttgtaata ccatataaag taataaaaga tttgtaaatt gaatagtagt ttatatataa
     3661 atcttttaag gaggacaata tgactacata taaaaagctt aaaaaatcta tatttaaaat
     3721 tgctatattt ttaatatgct tattagtttc attagtagat ataaacccta tatttgcagg
     3781 tagttcctct aatgagactt caaatagtga tacgataaag gtgttaaatg ctaagagtat
     3841 caactttaaa caagacaatt atgaagtgaa tgtgagtatt cctaaaataa aaggtttaaa
     3901 agataaaact ttagagaata aattaaacaa tgagtttttg caagatggga aaaaaattta
     3961 cagtgaattt caaaataaaa tgaaaaagga aaataataag ggtcatagat acctaaatgt
     4021 aatccataat gtcaaaaata attctaagga ctttttatca atagaaatga caaaagaaga
     4081 gatagaagct tcctcatcta taactaagaa gcattacact gttgataaaa atagaggaat
     4141 actattaaat ttaccaatat tttttaaaga tagtagatat attagatcta taagtgataa
     4201 tataaaagag cagatgagaa atcaaattaa gaaagatgat acaaaatctt attttataga
     4261 ccaagataaa gactccccag tgaaagattt tgaatctata aataagtatc aagattttta
     4321 ttttaataaa aatggaaatc ttgtgattag ttttgatgaa tatgaagtag cacctggtta
     4381 tatgggagca gtagaatttg tgataccaaa taaagtaata aaaagactaa gatagatata
     4441 tagaagattt ttagatagac tggtaaggag aattatatga atatatatga acaagcaata
     4501 agattaatag aaaataatga ggatttcgca tttgctacaa taacgtctca ttcaggttca
     4561 acaccacgtg aaactggagc aatgatgatt gtgaaaaatg attctactat atttggctca
     4621 gtaggaggag gtagtgtaga agcagaatgt ataaaacatg caattaatgt tataaaaaac
     4681 agagaatcaa tgttgtataa atttactcta aacaaatctg atgttgcaaa actaggaatg
     4741 atatgtggag gtactggaga aatacaaata gattttatag atagtaaatt aaaaagtaat
     4801 atagaaaaat ttaataagag actaaaggaa aatacaagta aagcatatat atttggtgca
     4861 ggtcatattt caagagatgt tgctgtgata ctatctcttt tagaatttag aacagtagta
     4921 atagatgata gagaagaatt tgctaatcat gaaaggtttc cagaatcaga agtagtagtt
     4981 ttagattctt ttgaaaacat accagatttt ccaactgacg aaaatagtta tataattatt
     5041 ttaacaagag gtcatctata tgattcaagt gctttagaat gggctcttaa aagagaggct
     5101 ggttatatag gtatgattgg aagtagaaca aaaattggat tgacttacga aaaactcatg
     5161 aaaaaaggtt ttaagaagga agaattatca aaagtacatg caccaatagg cataaaattg
     5221 aatgctcaaa caccagctga aatagctgta tgtatagctg ctgaacttat aaattgtaga
     5281 gctaataagg aaaaataaac taatgaacac agggagtaaa gatattgata ctttaaaggt
     5341 taattttaga gggattgaaa taaaagcagt tttagtatat aaaaatagaa aaaatatttc
     5401 tataaaaata gatccatttg gcaaaataac tgtaatgagt cctcctaaaa tatccaaaaa
     5461 aataataaaa gatataattg ttgaaaaagg agactggata cttaaaaagt cagaaaaata
     5521 taaaggaaga gaggaagtat ataaacaaag aatgtttata actggcgaaa aatttcttta
     5581 tttaggtgaa gagtaccatc tagttataaa agaactgttg agagattctg tcaaaaaaag
     5641 taactgtaga ataactatta acgaatatca aatagttgtt cagacaaatg atacaagtac
     5701 agattttatt aaaaaatctt taaaatcttg gtataagatg gaaagtgaaa gaatagtatt
     5761 agagagaata gattttctta aattaaattg tgaaataatg aagcaattag ttccagcgag
     5821 tgttaaggta aaagaacaaa ataagagatg gggaagttgt acagctcaaa aaaatattta
     5881 tataaactca aaaatatcta tggcaagatt agatgtaatt gattatgtaa taatacatga
     5941 gtttagccat ttagtacata tgaatcactc taaaaaattc tatgatttag taaaatccat
     6001 tatgcccgat tataaagata aagaaaattg gctaaaaata aatggatata aaataataat
     6061 ctgaaaacga ttattccaaa atataggcgt aaagtataaa agaagtatta ttgtgaaaaa
     6121 aataaataat ctgaaaattt taaaaaaagt cattgacatt gtaaatgata gggaatataa
     6181 tctatattaa caacaaaaca gaattgaaat ataaaattaa aaaattaaaa gcaatgacag
     6241 agacaacaat actaatagtg ttatccacag agaaccaatg ttgctgagaa ttggtgttaa
     6301 cagtagtata atgatcactc tggagctttt gtactgaata tttttagtaa gtacaaacgt
     6361 atgtcaacgt tacatgacta aggttaaggt aattaattac ccgacctgaa aaaagagagg
     6421 tagatgtatt ttttatatct acaaataagg tggtaccgcg gaatataact ttcgtcctta
     6481 taagtaatat ttatattgct tataaagatg aaagtttttt tattttaagc gatataaata
     6541 aatttaatcc atataaaatt aataaaaaaa taataaaaat aaggagggat ttttatggaa
     6601 aatactgtat atgctaaatt aaaccaagga atagattctt ttttaagagt ggctatgact
     6661 ctaagaagaa gagaggttga tatacaatca ataagtatga ctgtagataa tatgaataat
     6721 tcaggtataa aattaatagt aaatgaagaa aagacatctt tagattctgt cttaaaccat
     6781 atgaaaaaac tacatgatat aagagagata actgttgaaa gaatgagaca ttaattaaat
     6841 atattttata aagctaa
//
LOCUS       DAFKMX010000192         6806 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFKMX010000192 DAFKMX010000000
VERSION     DAFKMX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.195"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..686
                     /locus_tag="LZB08_003213"
     CDS             <1..686
                     /locus_tag="LZB08_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBM7316680.1"
                     /translation="IQIGAIKLDSKFNIIDTFNSYIKPTIYKDIHPFISRMTNIKNSD
                     FNDSPTFPEVYNNFIKFISSQDPILCVWGAGDLKELYRNINYHKLPSNSLPKSYINIQ
                     QHASKYFNNPAGKSIGLQNAISILELDEKMSYHNALNDAYYTAKVFIKIYNPSIVPDI
                     YLYTSIKPKTIRYSNKKRVDYDKLFDEFRKILNRELTKDEKKIINLAYNMGKTNQFTL
                     ENVKQRKNK"
     gene            complement(778..1530)
                     /gene="xth"
                     /locus_tag="LZB08_003214"
     CDS             complement(778..1530)
                     /gene="xth"
                     /locus_tag="LZB08_003214"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBM7316681.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1691..2641)
                     /gene="galT"
                     /locus_tag="LZB08_003215"
     CDS             complement(1691..2641)
                     /gene="galT"
                     /locus_tag="LZB08_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBM7316682.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3026..4222
                     /locus_tag="LZB08_003216"
     CDS             3026..4222
                     /locus_tag="LZB08_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7316683.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4557..5009)
                     /locus_tag="LZB08_003217"
     CDS             complement(4557..5009)
                     /locus_tag="LZB08_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316684.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5320..6765)
                     /locus_tag="LZB08_003218"
     CDS             complement(5320..6765)
                     /locus_tag="LZB08_003218"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBM7316685.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT         2292 a         1083 c          820 g         2611 t
ORIGIN      
        1 ttatacaaat aggagcaatt aaattggatt ctaaattcaa tataatagat acgtttaata
       61 gctatataaa acctacaatt tataaagaca tacatccctt tattagtcgt atgactaata
      121 ttaaaaattc tgattttaat gattcgccta ctttccctga ggtctacaac aattttataa
      181 aatttatatc atcacaagac cctatattat gtgtttgggg agctggagat ttaaaggaat
      241 tatacaggaa tatcaactat cacaagctac catcaaattc cttacctaaa tcttatatta
      301 atatacaaca acatgcatct aaatatttca ataatccagc aggaaaaagc attggcctac
      361 aaaatgctat ttctatattg gaacttgatg aaaaaatgtc ttatcacaat gctttaaatg
      421 atgcatatta tactgctaaa gtttttatta agatatacaa tccaagtata gtcccagata
      481 tatatcttta tacgagtata aaacctaaaa ctataagata ttctaacaaa aaaagagttg
      541 attatgataa gttatttgat gaatttagga aaattttaaa tcgtgaatta actaaagacg
      601 agaaaaaaat cattaactta gcttataata tggggaaaac aaatcaattt acattagaaa
      661 atgttaagca aagaaaaaac aaatagagtt tatttagata aaaaataggc atgtagatta
      721 aaaaactagt taactttaaa tttatatcta cagcccaatt tttatcataa ttaattttta
      781 ttttatctct aattcaactg gacaatggtc tgaacctaat atttcagtgt gtatatccgc
      841 acttactaat ctatcttcta attttttgga tgcacaaaaa taatctattc tccaccctgc
      901 attatttttt cttgcattaa atctatatga ccaccaacta taaacacctt ctttatcagg
      961 attaaaatat ctatatgtgt ctataaaccc actgtctaat aatttagaaa acttctctct
     1021 ttcttcttta gtaaaacctg catttttcat attattttta ggatttttta aatctatttc
     1081 tttatgtgca acgttcatat ctccacacac aattacaggc ttatgattat ctagtttggt
     1141 taaataatct ataaagtcgt cttcccatct agttctatat tctagtctct ttaattcaga
     1201 ttgagaattt ggcgtatata ctgtaacaaa gtaaaaatct tcaaactcta gagttatcac
     1261 tctaccctct ttgtcatgtt cttctatatt tattccatac attactttta aaggctcttt
     1321 ttttgtaaat atagcagtac ctgaataacc ctttctctca gcataattcc aatattgaaa
     1381 atatcctggt aaatctaact ctatttgccc ttcttgcaat ttagtttctt gtaaacaaaa
     1441 tatatctgca tctacttctt tgaaaaagtc taaaaatcct ttcccaacac aagctcttat
     1501 tccattaaca ttccatgata taaatttcat acttatactc ctcatctaaa cattgacaca
     1561 ctccccatag ctaaagatgg gggcttttca acaattttat tgtaaattct taattcactt
     1621 ttattttaac aatgctatac tataatttca tctaaaatta ctatttactt aatacctaaa
     1681 aatatatgtt ttaatcaatt tttaactttt ttgttaaatc ttcagggctc attgaagaaa
     1741 catacatatt tgttccaagt tcaaatcctc caaaattata ttttcttggg attatgtgca
     1801 tatgaacatt aaaatacgat tttgtttcaa tctttgttgg atgagtgtga atacataagt
     1861 taaatggcat atatccattg atattgtata acttcttaaa caacttttca aatattgtag
     1921 aaagctcctg tatattatta tcatttaact caccaaactc tattttatct ttaaatatta
     1981 ttcttacttc tccactatac ttggttgcat atggtataag taccaaaaaa gcttctccat
     2041 tataaacaac tcttctatct aaacttattt cattttctat taaatcatca tacaaagagc
     2101 ttttattgct gtcataatac tcctttgaaa ccaacagttc attcgtaatt tcaggtggaa
     2161 taaaagacat agacaatatt tgagcatgtg gatgcatcaa agatgctcct gcctttctca
     2221 agaagttttt gaagatacta acatactcta ctttatcatc tttcgacaaa tctctaaatc
     2281 tatttctata cataaaaaaa acatcttcaa attctttttg actcatgtta taaaaactac
     2341 gattatgttt atcgctctct atcattactt catgaacacc atatacatct cttgccatat
     2401 catcaattat aggaaattta ttgtagacag attttacaag ccatccttct tcactatcta
     2461 tttcaaataa tgcatcagtt gcatgagctt catttccttt gcaaaatggg cattcttcat
     2521 catattcatt agtttcttca tcttcatttg ggatatctgc tgtatctaaa ggtcttttta
     2581 acctatctgt agcaaatata acaacatcat tggtaattgg atctattctt aattctttca
     2641 taattaacac ctcttagtta atagccttta atataattat atgatattta tcgtatctgt
     2701 taaataaatt tataatattt tttataattt tgcatattta ttattatata ttttgtctat
     2761 agctgaaaga ttataactaa aaaatcacta tcagtatatc agaatattat cttactataa
     2821 attaatttat gaatacataa tttagtggca taaacatggg tattttacta taatttgtta
     2881 aaagtttatt ttaaagctat attaattttt tttgatgatt tttacccata agtatgttat
     2941 tattatcttg taaagatgtc ttgtattcaa acatacaatt tacataatac aatatacgta
     3001 tttaaattaa atagggggta ttaaaatgaa ttattcaaac agagttagtg caatgcaagc
     3061 ctctccaatc agaaaacttg ttccatttgc acaagcagca aaagataaag gaatcaaagt
     3121 atatcactta aacataggac aaccagacat aaaaactcct aaaggatttt ttgatgctgt
     3181 taaaaacttt gatagcgaag tcttagaata tgctacatca gaaggtattc cagaattatt
     3241 agaagcactt caaaattact ataaaactta cgatatgaat tttgaaaaag atgaattatt
     3301 agtaactaat ggtggtagtg aagctttact tttcactatg atggcagttt gtgacccagg
     3361 agataatcta ttagttccag aaccattcta tacaaactac aatggatttg gtcaatctgt
     3421 aaatgtagaa gttaatgctg ttacaacaaa ggcagaaaac ggtttccacc ttccatctaa
     3481 agaagaaata ttgtctaaag tagatgataa aactaaggct ataattcttt ctaacccagg
     3541 aaatccaact ggtgcaattt atacaaaaga agaacttaat atcctagctg aaatagctaa
     3601 agaaaaagat ttatggataa tagcagatga agtttataga gaatttgttt atgatgggct
     3661 tgagtataca agctgtggta acttagaggg tgtacaggat agagttataa taatagacag
     3721 tgtttctaag agatacagtg cttgtggtgc tagaattggt tctatcgctt gcaaaaataa
     3781 aggtcttatc gctcaaatac ttaaattatg tcaaggaaga ctttgtgttc ctacattaga
     3841 gcaaatagga gctgttgagt tatacaaaac tcctgtttct tacttcaaag aagttaatga
     3901 agagtacaaa aaaagaagag atgtattata caatgaatta atgaaggttg aaggtgttat
     3961 atgtaaaaaa ccaactggtg ctttctatat agttgctaaa ttaccagttg aaaatgcaga
     4021 agatttcaca atttggatgt taaaagaatt taacaaagat aatgaaacag ttatggtttg
     4081 ccctgctgaa ggattctatg ctactccagg tttaggacgt gatgaaataa gattagctta
     4141 catcttaaat gaaaaagacc ttcatagagc tgctacatta ttaaaagaag gtttagagca
     4201 atatgtagct ttacaaaaat aattttttaa acttaattta tataaattta atgatataaa
     4261 atgtcccctt ttcagatatt atttgaaaag gggacatttc ttaattaata tattaattaa
     4321 gacttagacc tgactatttt tcattttctt tatatagtgt tggccagtaa tctttattca
     4381 ctttaattaa agcatctaaa acttgtcttg ccttagttag agaaattata aaaaagtaca
     4441 tcaagaaaat ttaagatgta ctttttagat tgtatttaga cttataatta tatagttaaa
     4501 tgaataacaa agaattaatc ttagtaccat taaatacaaa aatttttata ttttttctat
     4561 ctaaattctt ctttgtttaa tatatattga agagtattta ttgcttctaa atattttttt
     4621 ctgtcttctt cagttaattt agaaagtttt tctcctaaac catcacttaa tttatccata
     4681 aaatcagttg ctatcttatc tccctcttca gtaatgtcaa ttagatattt tcttctatca
     4741 actaaatctc ttttccttgt tacatacttc ttatcagtta aatcatcaac catactagtt
     4801 aaactgcctt tttctatatt taacttagag cataaatcag tcatacttat tactccataa
     4861 ttttttatga aaactaatgc tcttaattgt gttttattta cattataatc tggtgcaaat
     4921 ttttttaggt cttctaagta caggctagaa taaatcttag gaaatgtttt tactaaaaaa
     4981 cttatggtat cttttactat atagttcata taatcacctc ggttgtttta aaatttatta
     5041 aattatctaa ataactttat ttcagtttac attaaaaata ttgcaagtac aatagttttt
     5101 gctgaatgtt taaatttaat tagtaatctt atcatttttt tatttttatt tatattattc
     5161 ctattaaata ttatttatat agctatttta ttaaattcat ggtatagtaa gtaaagaaga
     5221 actcttcaca gcggtggaga gaagacgcta gcccattaaa atggctatct tatttataaa
     5281 taagatagcc gtttttaatt atattttatc tgtacaattc tatactctta ggtctacatc
     5341 tacttttcct aagtcttcct tataatcttt tatagcaggt tctacaactt catttacaaa
     5401 ttctacaact tgttctggag ctctacctat aaagtttttt gggtccatta tagatagtat
     5461 ttcttccttg ctcattttaa atgaatcatc attcattata agttcaatta agttattatc
     5521 cttgccttca tgctttactc tataggcagc tttcatagaa tattctctta ttatttcatg
     5581 taattcctgt ctatctccac ctctttttac agcttccata agtattgttt ctgtagccat
     5641 aaaaggaagt tcttcattta cacgtctatt tatcatattt tcatatacta ctaaaccatc
     5701 agttacattt atccctattt caagtatagc atctgctgcc ataaatgctt gaggtattgc
     5761 caatctctta ttggctgagt catctaatgt tctctctaac cattgagtgg cttgtaccat
     5821 tgcagggctt atagattcag agattatata cttagataaa gatgctattc tctcacttct
     5881 cattggattc ctcttataag ccattgctga tgaacctatt tgattttttt caaatggctc
     5941 ttctaattct tttaaacttt gtaaaagtct tatatcatta gtcatcttat gcatactttg
     6001 agctatccct gatagtatgc ttattacttg ataatctaac tttctagtat atgtctgtcc
     6061 acttactgca taggaagaat ggtatcccat ctttttacaa attttcttat ctaactcttt
     6121 tactttttcg tggtctcctt caaataagct taaaaaactt gcttgagtac cagtagtacc
     6181 tttaactcct cttaacttca tgttgtctat tctatagttt aaatcttcca aatcaagaat
     6241 taaatcttga gcccataaac aagctctttt tcctactgtt gtaagttgag cagcttggaa
     6301 gtgtgtaaat ccaagagctg gcatatcttt gtacttaagt gcaaattctt ttaaattgtt
     6361 tattaaattt acaagcttta ctcttagaag ttttaaagct tcattcattt gtataacatc
     6421 tgtattatct ccaacatatg cagatgttgc tcctaaatgt attattcctt tggctttttc
     6481 acattgtaaa ccataagctt ttacgtgaga cattacatca tgtcttactt ctttttctat
     6541 ttttctagct tcatcaaaat ttatattatc tatattagct cttagctcat ctaattgttc
     6601 ttcagttata ttgattccta actcatgttc accttctgca agtgcaaccc ataaccttct
     6661 ccatgtagag aatttaaatt gtggagaaaa tatataactc atgtccttgc tacaatatct
     6721 gtctgtaagt gggtttgagt aagtagtata tttattatct atcattatat tagcaccttc
     6781 cttaaatctt ttgtcgtgtg attact
//
LOCUS       DAFKMX010000193         6657 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFKMX010000193 DAFKMX010000000
VERSION     DAFKMX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.196"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            321..473
                     /locus_tag="LZB08_003219"
     CDS             321..473
                     /locus_tag="LZB08_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316686.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(765..1469)
                     /locus_tag="LZB08_003220"
     CDS             complement(765..1469)
                     /locus_tag="LZB08_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316687.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1460..3067)
                     /locus_tag="LZB08_003221"
     CDS             complement(1460..3067)
                     /locus_tag="LZB08_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBM7316688.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3321..4499)
                     /locus_tag="LZB08_003222"
     CDS             complement(3321..4499)
                     /locus_tag="LZB08_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316689.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4725..5219)
                     /locus_tag="LZB08_003223"
     CDS             complement(4725..5219)
                     /locus_tag="LZB08_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBM7316690.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5220..5981)
                     /locus_tag="LZB08_003224"
     CDS             complement(5220..5981)
                     /locus_tag="LZB08_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBM7316691.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2262 a         1220 c          703 g         2472 t
ORIGIN      
        1 gacaaaaagg gcaatttttt tataccaaaa aaaatgttaa attgatatta tccatatata
       61 aattgatatt atccatatag gtgctatatt taacaattct acttacttcc cttacactta
      121 ttagcatata gttattaaga gtttgttgca aaacaacact tagcacactt taaaaagtgt
      181 ggtctaataa gtaatcaacg aagaagacat aataaagcat aaaatatgat atacttttcc
      241 acgtcatcaa aactgaggat tttattatta atatatgata aaatatgctt aataaattaa
      301 attttaatgg gagaatagtt atgaaaatat ttagtttctt gttgtttgta gtgggaatat
      361 gtttttgtat tgctggattt atggggcatt cctcagcttt ttctggtgca acattgagtt
      421 tttttgcaac tacaatgata gctgtaagac aagtcaaaac taagaaaaaa tgaaataggc
      481 aaatcctaac tttacaagtt aatattacat aagtaattag tctaaaatta gatacttttt
      541 ctattacaga aatacatcat ccactttgta ttctcttaaa aaaataaaat agctatgtaa
      601 attcaagatt agatagacta actctctatc catttcattg taaaatatac atagctattt
      661 gtattcgatt taaaatactt agattttctt acataacaaa taatttctat ctgattatta
      721 ctcatattca tttagtaatc actcagcaac taatcattta gtatctattc atccgccata
      781 cggtaaccta ccccaacctc agtcaaaata tattctggct gagcaggatt cttttcaatt
      841 ttccttctta tacttgccat aaaaactcta agcgactgat tctcactttc atagtaattt
      901 ccccaaactt tatctattat aaacttatgg gttaagactc ttcctgaata cttagacatc
      961 aactcaataa tcttatattc gattggagtt aaatgtattt cttcaccatt aattgacaca
     1021 atatgatttt cataatctat ctttaggttt ttaacttcaa aagactttgg agcatcatta
     1081 tttatattat cctgttgagc tttatttcta agagccacac gtactctagc caaaagctct
     1141 gttacactaa aaggtttagt taaataatcg tctgctcctc catctaaagc ttccacctta
     1201 tccctctcat gctctctcgc tgaaacaaca attattttat tagtctttgc acatgcccta
     1261 actcgtccta ttacctcaat cccatcaata tctggtagtc ctaaatccaa aattatcaaa
     1321 tctgggtcaa gagaatgtat aagtgatata gctgttcctc cattatctgt ctctacacac
     1381 ctataatttt gagtttcaag agatactttt ataaatttac aaattggctt atcatcttct
     1441 acaattagaa ctaatggctt tatttccact tgtctttccc ctcctcatca tacataggta
     1501 tgctaaatct aaatgtagct cctccagaag gattattaaa ggcgacaatt tctccaccat
     1561 gagcttctac tatagattta catatctcca atccaagacc aatacctctt ctttcaagat
     1621 ttctagactt attcttagta taaaacctat taaaaatatt cccgatatct tcatttggta
     1681 ttccttttcc attatctgca acttcaaata gtacttgatt tgactctttt tttacagtta
     1741 gttctatctt agaattttta ggtgtatgcc ttatagcatt atcaattaga tttactaaca
     1801 cttgttctat taataataca tccacatgaa cgattattat ctcatctgga atatttgttt
     1861 ttatgtctct actattggca aactttttga ctcttaatat agattcagct attatttcat
     1921 caacaacttc tggtcgtttt tcaatatcca attttccttc atctattctg gtcatactaa
     1981 gtatattctc aacagaatga gttagccaac tcgtatcttc atatatattt ttcaataatt
     2041 ctactctagt ttctttatct atcacatcat cattttcaag tattgttgaa gatgaaccta
     2101 atatacttgt aagtggagtt cttaaatcat gagaaattga cctaagcaag tttcctctta
     2161 atctctctct ctctgcctca agatttgctt ttttattttt ctcaaataaa ttttctctat
     2221 caattgccaa cgcaacctgt gttgaaactg cttctaatag tatcttttca ttttctgtaa
     2281 taatatctat cttaaaacat gcaacaccta taacaccaag aacatttgat tcttgacctt
     2341 ttatagggaa ataatatgca ctgctgtttt tacaatattt tgtcccaatt ccaactgggt
     2401 tacctgtcct aaaagcttct tttatagctt ccttttctaa tactgaatgg aatatattct
     2461 cacttccatc attgttacaa atataaatac ttggcttacc tagattatta ttctcatctg
     2521 caatagtcac tattacagtt ctatttaata tatttactaa attagaccca caaaattcaa
     2581 taatttgatt tttatttttt gctttaagca acgttttgtt attctcatag agcaatctta
     2641 tcattttttc tctcttatga gaaagtctca cttctctttt tatcctagaa gttaaagtac
     2701 ttgttataat tgctgccatt agcataatac aaaaggtcat aggataatct tttctataag
     2761 ctaaaaaagt atataatgga tgcgtaaaga aaaaattaaa tgatagcact cctattactg
     2821 aagaaagtat tccaaaaaaa tatccatagg ttatacttgt agcaaatagt acacctaata
     2881 tgtatactag tataatatta gattcattaa aacctacctt ttcaaaaact actgatattc
     2941 cagtagcaat tgtcataacc aatatagttt ttatgaagtc cttaataatt atctttttac
     3001 tgagatagcc atcttcatca taatcatact cttttaagtc ttcatgttgt agtcgttcct
     3061 ttttcattta ctacaattcc tccttattgt tactgtttaa attggctcta ttttatataa
     3121 tacaatttta tcataataaa ttcaatcaat acatttaatt aatgtactta aattactaaa
     3181 aggtgctgtc ttaaaatgaa aataaatctc tatttcaaaa cagcacctat atatttaaat
     3241 tactaattta gtttttacat acttaaatca atagttttat tcaatttcat aaattataat
     3301 acttcaatta taaaacattt ctatccaaca agcataaata taaagtgggc acatatacct
     3361 gcacataaac cacctatagt atgcgaaagt atcgctttac cagccaatgg tcttgcatct
     3421 aaagcatcca tcatacctat gtgtgtactt aaatatccac tccaacacat acccattgct
     3481 gtgaatactg ctatatcatt aggagttata gcgcctgatt ttataaactc tggtactaaa
     3541 gatattgctg ctccaacagc tcctaatgca gttacaggga atgctattgc ctctggtgat
     3601 gtgaatccaa ataatggttc tattatgaag ctaatcttat caccaatagc aggtaataat
     3661 tttatacctt catatgcagc tcctgtgtaa acagcttgac ctgtagcagg gtctgtagat
     3721 ggtccaaaag ttaataacat aactaatgta caaactacta aaactcctgg tattatagcc
     3781 atacccattt caacacctaa cttaccacct tcaagtatag catccaatgt tctttggaat
     3841 acattaccat ctcttatttc tctaaactct tcaagttttg cacctttatc atttatttgc
     3901 ttttcagtat catttttagg gtcataatta taatactttt tagtaaaagt caacataatt
     3961 ctaacactaa taatactacc tattattgcc gcaacattac ctattatagc tggtagtaca
     4021 tattctttac cttgagctat cataaaagtt gttacaataa gtcccatacc aaaggctgtt
     4081 cctaaattac ataaagcagg tacttgatac ttcttaaaat attgtgtaaa tgttttgtct
     4141 ttagcaaatg gtataatagc tggattatca gataaatatg ttgcaactac tcctgctata
     4201 ctagcaccag gtaagcccca tataggtctc ataagaccct taaatatctt attaactaaa
     4261 gatattactc caaactctga aagcaatgca ctaagtgcac ctgcaagaac tgccatagcc
     4321 attattaaaa atactgtgtc taataaaagc gcatgtgctg tactcattat tactttaaac
     4381 atcattcctg cacccatgat gcttcctaca tatccaaatc ctaccgccaa tagtacaata
     4441 aatacgaagg tttccatact tactgccttg actcttctct tatccgacag cttttccatc
     4501 ttgaaatctt ccccctttta attttaaatt aaggttccta acattggtac aaattttttg
     4561 gtacaaactg agttctaaaa cttacactcg taatgcaagt tgtcagtttt ttgaaataaa
     4621 aaaaaattac gtttatagaa taagaatatc ctttttaatt tggatactct tattctattt
     4681 tatgttatta tattatcatc cttttgttta aaagtcaatt ttttctattt ttgttgttta
     4741 attttattta tactcttttt atatttttca ttttctttta tctttgtaag tactccatat
     4801 aagttgactt ttctaaatac agtagttata aataccaatc ctaatgcaat tgaacttgca
     4861 cacgataatg tactcacccc atattctaca gcttttatat aattattggt caaaacagaa
     4921 tacatagtat tatatattcc ataaccaggt actaatatta ttaaacagca aacacttaat
     4981 gtagttgctg gagtcatata aattcttgca cataactcac aatatatcgc aaaacaaact
     5041 gtagctataa aaaaacataa gttttctgtt actcccatat ttaaaagaaa tttatacgaa
     5101 aaccaagcta tacttccacc tattcctgct agtattaagt tttttccttt catattaaat
     5161 agcactccaa agcttaaggc agtaaaaaaa gctgctatta cttctataaa caaatgcact
     5221 tatatccccc ctaacttgaa gtacaatcca agtaccaaga acattcctac tgctatagat
     5281 acacctacaa aaaatacttc tgctaatttt gcaagaccag aaatcaactg tccatctaac
     5341 aaatctctta atgcattagt caaagcaagc ccaggaacta agagcattat tgctcctgct
     5401 attaaatggt ctaacgtctt tatgaatccg agctttaaaa ttaaaattga acaagaagct
     5461 ataataactg ctccaattgc atttacaaaa aagtcattaa gggaagattt acttaaatac
     5521 ataataacaa acttagtcag aatcccaaca gtaaatgcag ctataaattc ttccacattt
     5581 cctccaaata atatcgaaaa agttgctgct gcaatacctg caaaaaatat tgttattaaa
     5641 tccgagtaat agttatcttc acttatttca tctaaaattt tctcacataa ttcaatatct
     5701 aaattattta tacttattgc tcttgataat gagttaactt catgaaccaa gtttaggtct
     5761 actcttcttg agcttattct tttaattgct gaatgtaatt tcccatctac aaatacagat
     5821 gccattacag ctgttggagt tgcaaataca tctacgtggt ctattccaaa agattgacat
     5881 attctagcta tcgtttcttc aactctatag gtttctcctc cactttgcaa catagcttta
     5941 cctgcatttg aggaaaagtt taaaattcta tctatatcca caaaacatat ctcccattct
     6001 tttatagtat tattcatatt ttaatacaag atattattta tttaaactat tttaaataat
     6061 tcatatccaa tatttacatt ttatgaaaaa acttgttttt tatgcttgtc aaaatcaaat
     6121 tttaagcttg tttacaccat aaaatggctg aattaatatt tgacaaacac tttcaaccat
     6181 ttatataacc ttctaaattg tataactttt tactacaaat aaaattatat ctcttttaat
     6241 tctttctctt gagtatttag cttatagtaa cttcatattt atatatgtac aatataaaac
     6301 attattttta tgatacaaaa tataatttgt aaatgtatat atgtattaac tgtgtatata
     6361 taaattattc ttaaccttat cagttttttc atatatcttc atctataccg accatatatt
     6421 aattacattg accatatatt aattacattg accatatatt aataaatgat attacataga
     6481 cttaagtaca ttaaaaaact taaatttagt tatttaacaa cataatactc taatttaatc
     6541 tataaaaatc catacctaat ataatgcaac tcttatttta acaccatcta ttacacaaaa
     6601 caacatatta aaaagtaaaa tacaaaatat ctactataaa ccttatttta atactga
//
LOCUS       DAFKMX010000194         6469 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFKMX010000194 DAFKMX010000000
VERSION     DAFKMX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.197"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            285..1229
                     /locus_tag="LZB08_003225"
     CDS             285..1229
                     /locus_tag="LZB08_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBM7316692.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1251..1877
                     /locus_tag="LZB08_003226"
     CDS             1251..1877
                     /locus_tag="LZB08_003226"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBM7316693.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1911..2960
                     /gene="hisC"
                     /locus_tag="LZB08_003227"
     CDS             1911..2960
                     /gene="hisC"
                     /locus_tag="LZB08_003227"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBM7316694.1"
                     /translation="MREKESIRELRGYEPNHVNCEVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEI
                     SMFVGAIRDILKKEK"
     gene            2963..3541
                     /gene="hisB"
                     /locus_tag="LZB08_003228"
     CDS             2963..3541
                     /gene="hisB"
                     /locus_tag="LZB08_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBM7316695.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3543..4148
                     /gene="hisH"
                     /locus_tag="LZB08_003229"
     CDS             3543..4148
                     /gene="hisH"
                     /locus_tag="LZB08_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBM7316696.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLRLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4145..4867
                     /gene="hisA"
                     /locus_tag="LZB08_003230"
     CDS             4145..4867
                     /gene="hisA"
                     /locus_tag="LZB08_003230"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBM7316697.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4849..5607
                     /gene="hisF"
                     /locus_tag="LZB08_003231"
     CDS             4849..5607
                     /gene="hisF"
                     /locus_tag="LZB08_003231"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBM7316698.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5635..6303
                     /locus_tag="LZB08_003232"
     CDS             5635..6303
                     /locus_tag="LZB08_003232"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBM7316699.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2642 a          542 c         1264 g         2021 t
ORIGIN      
        1 agaatattaa taaaaattat ttgggaggaa attatcatgg tgagattaaa tatagtaaaa
       61 ttaaatacaa aattaaatca tcaccatcat catagattaa agggattgta atatgaaatt
      121 aattcataga cacaagccct gtttttagtg taattggtct tgtatctatg atgggaagta
      181 attttaagag aaaattatag ccatataggt ataaagccta tatggctttt ttattgtttt
      241 aaatttgcaa atataagagt aaatttaatg aagagggtga gaatgtgaaa tttttgagga
      301 ttgaagatga ggttatttat tctaaaaaaa gatataaatt aaaaagggaa atagataaac
      361 tttttttgga tgaagaatat tttcaaatag aaccacaatt atttgaagaa tatgatgaat
      421 ttacaactat aaataataaa attccagaag aatctatggt aaaggttgta aatggaaaag
      481 taatggtact tagagctgat ataactacaa gtataataaa aagccttgta ccaagatggg
      541 aagatggttt aaagcttaag cttttttata attcatcaat atataaaaat aaaaatacag
      601 ttggaataaa agagataagg caaataggtt gtgagtatct aggagaagcc tctgtagaag
      661 cagatagaga agtagtgaaa ttagctctta agatacttga aaaatacaat aataatttta
      721 ttctagaggt tgggagtagc aagtatattc atggtttgtt agaagaatta aatttaaata
      781 agaattgtga aaatcaaatt aaaaatttac tatatacgaa gaacactcat gaattaaaag
      841 tatatattga agaattgaaa attaagcgtg aagtaaaaga gttattatca aatattttaa
      901 gtttacaagg gaatttatat aatgttattg aaaaatcgaa caagttctat tgtaataata
      961 agatgaagca agctcttgaa gaattaaagc aagtaaataa tttaattgag gaatgtaatt
     1021 ttttagataa ggctagattt gatttatcaa tgattactat gcttgactat tatgaaggaa
     1081 ttatgtttag aggatactac ccaaactcat ataaagaaat tcttagtggc ggaagatatg
     1141 attctttaac taaagagtat ggaaaagaaa ttccagccat tggatttaca ctaagtgttg
     1201 atgagttaat gaagtatgta tataagtaaa gtttagaagg agggattgtt ttggattatt
     1261 tgactatagc actagcaaaa ggacgaattg aaggggaatc atttaaaaaa tttaaaaaaa
     1321 tgggtctagg agatagtata gacacagata cgagaaaact aatttttaaa gatgaagaaa
     1381 ataaaattat atatatacat gtaaagccat cagatgtagt tacatatgtt gaaaaaggag
     1441 tcgctgacct cggtatagca ggtaaagata ctattttaga aaatgagaca gatgtatatg
     1501 aaatatatga tttaggtttt ggaaaatgta aatttgctgt agcaggatta aagggagata
     1561 gcatatatag agaagatgaa tatttaaaag tggcaacaaa gtatcccaat atagcaaaaa
     1621 agtattttaa agagaaaggt caaaaaattg aaataataaa attaaatggt tctgtagaac
     1681 tagctccaat agtagggttg tcagatgtaa ttgtagatat agttgagact ggcaatacac
     1741 ttaaagcaaa tggtcttgaa atattagaag acatatgtaa tataagtgct agaattatat
     1801 caaatagagc tagctataga tttaaatatg aacaaattca gaatataatt cgtttatttg
     1861 aagaacttga taattaaaat ataattgagc tttataagga gggatttaag ttgagagaaa
     1921 aagaaagcat aagagagtta aggggatatg aaccaaatca cgtaaactgt gaagtaaaac
     1981 ttgatgcaaa tgaaggaagt aagaggttgt ttaaatacct tattaaagaa atttcagata
     2041 gtgatataga tttaaattta tatcctgaag attcttatag tgatttgaaa gaatctataa
     2101 tagattacat aaatatttct ggagtaaata agaaaaatct tctagtagga aacggttcaa
     2161 gtgaaataat tgatttaata atacatactt ttgtagataa agatgaagta atattgagtt
     2221 tctctccaag ttttagtatg tattctatat atagtcagat aaatgggtct aaatttatag
     2281 gtgttgaaag tgatgaaaat ttagttataa atatagataa tgtgattgaa aaagtaaagg
     2341 agaataatcc taaaatagta attgtatgta atcctaataa tccaactgga acaatattaa
     2401 agagggaaga gataataaag cttttagatt caactaatag tctagtggtt ttagatgaag
     2461 catatatgga ttttggagaa gaaagcatgt taagtgatgt ttttaagtat gataatctaa
     2521 tagttcttag aactttatca aaggcatttg gattggctgg aataagaaca ggatatatgt
     2581 tatctaatag tagtttaata aattctgttg agaaagtaag acctccatat aatttaaatt
     2641 cattatcaga ctttattgct acaagagcac ttagaaataa agatgtggta aaggcttata
     2701 taaaggaagt aaaagaggaa agagaagttt tatatgaaga aatgattggt atgggaatta
     2761 aagcatacaa atcacaagca aattttatat tattttattc tgaaatcgaa aatttatcac
     2821 aaaaacttat tgatagaggt gttttaataa gaaaatttgg tggaaagtta gaaaattatt
     2881 acagagttac aattggtgat aaagaagaaa tttctatgtt tgttggagct ataagagata
     2941 ttttgaaaaa ggagaaatag gtatgagaat ttggaaggta gaaagaaata ctcttgaaac
     3001 acagatttta gtagaattaa atattgatgg ttctggtaaa gctgaaatag atactggaat
     3061 aggattttta gaccatatgc ttaccttaat gtcatttcat ggcaagttcg acttaaaagt
     3121 gatttgtaag ggtgatacat atgtagatga ccaccatagt gtagaagata taggtatagc
     3181 tataggtgaa gcatttaaaa atgctctagg tgacaaaaaa ggaatacgaa gatattctaa
     3241 tatctatata cctatggatg aatctctatc aatggtagca atagatataa gcaatagacc
     3301 ttatcttgta tttaatgcta aatttgatac ccaaatgatt ggtagtatga gtactcaatg
     3361 ctttaaagaa ttttttagag cttttgtaaa tgaatcgaga gtaacattac atatcaatct
     3421 cttatatggg gaaaatgacc atcataagat agaaagtatt tttaaagcat ttgcaagagc
     3481 tttaaaagag ggtagtgaaa tagtttctaa tgagatagca tcttcaaagg gggttttgta
     3541 gaatgaatat aatagtagac tatggtcttg gaaatataga ttctgtgtct aggggtttta
     3601 gaaaagctgg aatagaaact aaaatttcta gtgatataga tgaaataaaa caggcagatt
     3661 cacttatact tccaggtgtt ggagctttta gagattctat aagtgcctta gataaactaa
     3721 gattgatacc aataataaag gagcatgttt ctaagggtaa atttatgata ggtatatgtc
     3781 taggaatgca attactttat gaaaaaagtt atgagtatgg agaatatgaa ggattaggac
     3841 ttataaaagg aagtattgat aaactggata taagtttaaa agtgccacat atgggatgga
     3901 ataatctaaa atttaataag gcaaatgatg atatacttaa atatataaat gaagatgatt
     3961 atgtatactt cgttcactca tattatgcaa attcatcaaa tgaagaacta attgcatttt
     4021 cagaatatga aaagaaaatt cctgctattg ttagaaaagg caatgtatat ggtatacaat
     4081 ttcatcctga aaaaagtgga gaagtagggt taaatatact aagagcatat ggggagatga
     4141 taaaatgata atatttccag caatagatat aaaagataat aagtgtgtaa gactcacaca
     4201 aggagaattt gacaaagtaa atgtatatta tgataatcca ttagaagtag catataaatg
     4261 gaaaaatgaa ggagcagaat atatacatat agtagattta aatggtgcta gaagtgaatt
     4321 tggtgtaaat acaaaaataa ttgaggacat agcaaacaat atagatatac ctattcaagt
     4381 tggcggtgga gttagggata aggaaaaagt caaaagttta ataaatgctg gtgttactag
     4441 agtaatatta ggtagtatag cgattgaaaa tttgaattta gttgaagaat tggtcaatga
     4501 atataaagag aaaattgtag tttctataga tgctaaagat ggaaaagtag cagttagggg
     4561 atgggaagtt gtaagcaatg tagactcatt aacattatgt aaacaacttg aaaagatagg
     4621 tgtacaaact attgtttata cagatatatc aaaagatggg atgttgcaag gtccaaattt
     4681 tgacatatat gaaagaatag caaaggaaac atcactaaat gttattgctt ctggaggcgt
     4741 aacttctata gaagacgtaa aaagactaaa agctatgaac ttatatggtg caataatagg
     4801 taaagcgctt tatgataaaa agatagactt taaggaggca caacaattat gcttactagg
     4861 agaataatac cttgtctaga tgtgagaaat ggaagagtcg taaagggaaa aaaatttaaa
     4921 gatatagtag atgttgatag tccagaagta cttggaaaat tttatagtga ttgtggtgca
     4981 gatgaacttg tattctatga tataactgca tcaaatgaag agagaaagac atctttagaa
     5041 tttgtgacaa aagttgcaga aaatataaat ataccatttt gtgttggtgg aggagtaaat
     5101 aaattagaag atttcactga tattttaaga aaaggtgctg ataaagtttc tataaattca
     5161 tcagcagtca aaaatcctga attaataagg gaagcttcat taaaatttgg agcacaatgt
     5221 gttgttttat cgatagatgc aaagaaaaat gaagagggtt catggagcgt atacgtaaaa
     5281 ggtggaagag aaaaaactaa tctggatgca attgaatggg ctgttaaagg tgtagaactt
     5341 ggagcaggag aaatcgttgt aaatagtatg gatgaagatg gaatgaaaaa tggatatgat
     5401 atagaattat tatcaaagat aacttcttta gtaaatgtac ctgttatagc atctggaggc
     5461 gctggaaaaa aggaagattt ttatgaggcg gttaataaat ccaatgtaga tggaatttta
     5521 gcagcatctg tttttcattt tggcgagata aaaataaacg atttaaagaa atatttaaaa
     5581 gacatgggtg tagaagtaag attataagtt aaatactaaa aggagattag aaatatggat
     5641 aataagtgta acaatgtata cagtgatgaa gtagagaaat ttataagaag tataaagttt
     5701 gatgataaag ggttagtccc tgttgttgtt caagaggtag ttagcaaaga tgtactgatg
     5761 ttagcatata tgaataaaga tgctataaag aaaaccttaa aagataaagt agcttgctat
     5821 tttagtagaa gtagacaaga actttgggtt aaaggtgaga catctggtaa tactcaaaaa
     5881 gtagttaaga tgtcttatga ttgtgatgta gatactatac tactttttgt tgaacaaact
     5941 ggtgtagctt gtcatacagg caattattct tgtttttaca gagacttgtt tgatgatagt
     6001 tcaaaaatgg agcttgaagt acaaacaaat atattgaaag aattatatga tttaatcaat
     6061 gaaagaaaaa ataatccagt tgaaggttct tatactaatt atctttttga aaaaggaata
     6121 gataagattc ttaaaaaagt aggtgaagag agtagtgagg taattatagc atcaaaaaat
     6181 accgataaaa gtgaacttat ttacgaaatt agcgatttag tatatcatac attggtttta
     6241 atgatagaaa aaggtgttga aattgatgaa ataaaaaaag aattacttaa aagaagaaaa
     6301 tagaataaga tagattaatg ggtaataaga aaatatacca cttttaataa aaagtggtat
     6361 attttttatt ttatatttta atttatagaa taatatttaa attacttgat aaaattaact
     6421 atatatataa tttattgata aaataattct agtacattat ttattttag
//
LOCUS       DAFKMX010000195         6436 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFKMX010000195 DAFKMX010000000
VERSION     DAFKMX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.198"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            321..764
                     /locus_tag="LZB08_003233"
     CDS             321..764
                     /locus_tag="LZB08_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316700.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            795..2612
                     /gene="ftsH"
                     /locus_tag="LZB08_003234"
     CDS             795..2612
                     /gene="ftsH"
                     /locus_tag="LZB08_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBM7316701.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2790..5492
                     /locus_tag="LZB08_003235"
     CDS             2790..5492
                     /locus_tag="LZB08_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBM7316702.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5526..6224
                     /locus_tag="LZB08_003236"
     CDS             5526..6224
                     /locus_tag="LZB08_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316703.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         2543 a          710 c         1172 g         2011 t
ORIGIN      
        1 atatatggtg cgataatggg agactaaacc tttatagtat caataaattt gtattttaac
       61 ttttaaaaga gattaaaaag ggagaaatca gtgtaaaata tatatataga ttttttatta
      121 aattttatta aaaatatcct gtataaaatt aaatatcaat aagataacaa aatttcaatt
      181 gaagttacaa tattgtaatt gacaataaat acttaatact ataatattta atacattaaa
      241 tattatagta ttaagtattt atttacagta tgcacttcag cctaaatttg aaataaagta
      301 ttttcgtagg gagcgtttgt atggatgaga tgagttttaa gaaagagcta ctagagttga
      361 ctagagatat aaatatgaaa tttacaactt tattaagtga tttttatcag ccacttggta
      421 ttacagcagt gcaagctttg attttatctg agttatgtga acatggagag aaaaaaataa
      481 gcgatttagg gaagaactta aacatgacaa atagtaatgt atcagtaatc tgtcaaaggc
      541 ttgaaaaaaa tggattttta aatcgcataa gagatataga agaccaacga attgtaaaag
      601 taaaagttac aaataaatca ttggatatac aagaacatat ttcaagtagt atttttgata
      661 gttattttga aaatatgacc agtgaaaaat tgcaagatat ggaagatata atagaaggtt
      721 tagagaaatt aaataaactt ttaacttaca ttgattgcaa ataacataaa aataatttaa
      781 aggagaataa aattatggat gaacataaac cccaaaaaag gtcagcactc tattattata
      841 cagttataat aatagtagta atgtttttaa atgctttaat atttccaagt ttaatggata
      901 aaaaaatggt tgagattgac tatggaaagt ttctcacaat gttggataca ggaaaggtta
      961 aatctgttga gatagaaaat aatagaatag caataaatcc atctagtagc aaggataaga
     1021 atatatacat aactggtaga atggatgacc cagaattggt agatagacta aaaaatgcaa
     1081 aagtagaatt tactaaggtt attcctaagg aaaattcacc tcttttaagt attttactta
     1141 catggataat acctataggt attcttatgc tatttggtaa cttaatgatg aagtctatgc
     1201 aaaagcgaat gggtggtaat gctatgcaat ttggaaaaag caacgccaaa atgtatgtat
     1261 ctgctcaaag tggaaaaaga tttaatgatg tagctggtca agatgaggca aaagaagctt
     1321 taactgaaat agtagatttc ttacacaatc cagaaaagta taaaaagata ggagcacaaa
     1381 tgcctaaagg agcactttta gtaggacctc caggtacagg aaaaacactc cttgcaaaag
     1441 ctgtagcagg tgaagcaaat gtaccatttt tctcaatatc tggttctgag tttgttgaga
     1501 tgtttgtagg aatgggagct tctaggattc gtgatttatt taaacaagca aaagaaaaag
     1561 ctccatgtat agtgtttata gatgagattg atactattgg taaaaagcgt gataatggaa
     1621 gtggattagg tggaaatgat gaacgtgaac aaactttaaa tcagttgtta agtgagatgg
     1681 atggttttga tggtggaatt ggagtagtaa tacttgctgc tacaaataga cctgacagtc
     1741 ttgacaaagc attacttcgt ccaggaagat ttgatagacg tataccagtt gaacttccag
     1801 accttggagg acgtgaagct atattaaagg tacatgctca aaatgtaaat gtaagctcag
     1861 atatagatta taatgcaatt gcaaggtcta catcaggagc atcaggtgca gaacttgcaa
     1921 atattgtaaa cgaagcagca cttttagcag ttaagtctgg cagagatata gtagaacaac
     1981 aggattttga tgaatctgtt gaaactgtaa ttgctgggta tcaacgtaaa ggagctgtaa
     2041 tttcagataa ggaaaagaaa attatagcat atcatgaaat aggtcatgca cttgttgctg
     2101 ctatgcaaaa acattctgca cctgtccata agattactat aatacctaga acttcaggag
     2161 cattgggata tactatgcaa gttgctgaag atgagagtgt acttatgtct aaagaagaag
     2221 caattgataa aataactact tttacaggtg gtcgtgctgc tgaagaggtt atctttaaca
     2281 cttgtacatc aggagcatca aatgatatag aacaagctac taaaattgca agagctatgg
     2341 ttactcgcct tggtatgagt gaagagtttg atatggtcgc tcttgaaact gtaagcaatc
     2401 aatatttagg aggagatact tctcttgctt gttcttccga tactgctgct aaaatagatg
     2461 aagaggtatt taaaatagta aaggcttgtc acaaaaatgc aattgaaatc ttagaaaata
     2521 atattgataa attgcatgaa cttgctaatt atttattaga aaaagaaact attacaggtg
     2581 atgagtttat ggaaatactt aacttaaact aaaatgatta tatacaagat aaaaatgcct
     2641 attgttaggc atttttattt tacaactaaa ttttttaaat tcatatttaa atctttacat
     2701 aatcttaatc attaaaaata catcttaact acaattttat atcggaattg atattataat
     2761 agtaatgaat ggtgtaagga ggttttttaa tggatagacc taatcctgat attctattag
     2821 aaaaaattaa aaatgaagaa gaaaaactaa gtagaggtca attaaaaata ttctttggtt
     2881 atgcagctgg agtaggaaaa acctactcta tgttggaatc tgcacaaaat ttaaaaaagg
     2941 tgggtgtaga tgtagtagtt ggatatattg aaccacacac tagaccagaa acattggctt
     3001 tacttgatgg cttggaaatt cttcctgtaa aggagataga atataaatct ataaaactaa
     3061 aagaatttga tttagatgct gctttagcaa gaaaaccaga agttatatta gtagatgaat
     3121 ttgcccattc taatgttgct gggttaaggc atacaaaaag atggcaagat atagaagaat
     3181 tgctgttagc aggcataaat gtctacacta ctgtaaatgt acaacattta gaaagtctaa
     3241 atgacattgt tgagataatt acaaacgtat cagttaaaga gactatacca gataagtttt
     3301 tggatacaaa tacacagtta gaacttatag atgttgaacc tgatgtactt cttgaaaggt
     3361 tcaatgaagg taagatatat aagaaagaac aagctactag agcaaaaaat aattttttta
     3421 taaaagataa cttggttgca cttagagaaa tcgcacttag aaaaacagca gagagagtta
     3481 ataaagaagt tcaaatgacc agactatcaa agggtgatgt aactgtaata cctacatcag
     3541 atacacttct tgcatgtata agtccatctc catcttcagc aaaagttatt agaactgcat
     3601 ccagaatatc agacagtaca tttgcaaaat ggatagcttt atatgttgaa actccaaaca
     3661 cagcaagact tccatttgat gaacaaaaac aactacaaaa taacctaaaa ttagctaaga
     3721 aattaggtgg agaaataatt gttttacatg gtgaaaatat aatagagcaa atacttagaa
     3781 ttgcaaagct gagaaatgta acaaaaattg taataggtag aaatcattca agtaataaaa
     3841 gatttagtaa aagatttaaa aaagatatag ttgacaaatt aattgatgaa gttgattata
     3901 tagatataca tataattcca tataaatttg cctcagatgt taaatacaaa cctaaaaagg
     3961 ataaatcatc aataaaatct aaatttaaaa ttagcaaagt tgattttata aaacttatat
     4021 ttattacttt gatgataagt attttagcat atgcagtaca aagcatggga tttataagag
     4081 aaaatatatt gttaatatat atgttgggtg tagttttagt ttctatgtgg actaaagggt
     4141 atagtacagg tataataagt tctgtattta atatagttct tttaaattat ttttttacag
     4201 caccattata tacattatct atagcagatt caaactatat agtaacactg gttgtattct
     4261 caatagtagg tataataact agcactctta cgtctaagat acagcatgaa gcagaaactg
     4321 ctgctaaaag agaagaaaat acaaaaatga tatatcaaat aattaaggga tttttaagat
     4381 tatcaaataa agatgatata gtaaacaaag gtatagaact tttgtctctt agcttaagta
     4441 gagatgtaat ttgttattta aatgtagata agcataataa atcaaagctt tacaaaaaaa
     4501 atactaataa taaggataaa aatgatttgg atagtgaaga tgaaaaagct gttgcaagtt
     4561 gggttttatc caattcaaca gtggcaggaa atgacacgga tacacttcct ggttcaaagg
     4621 gatactatat accaattgta ggaatgaatt taactttagg agtagtggga atatcatgta
     4681 ttgattcaaa attggataca gaagatataa gcttaattga aacaataatt gctcaaatgg
     4741 caatagcgct tgatagagaa atcttgtcag aagctaaaga aaataccaat cttgagattg
     4801 aaagggaaag attaagaagt aatcttttaa gagcagtttc gcatgacctt agaactccac
     4861 tagcaggaat atctggtgca gtaagtacaa ttataaaaaa caagggtact ataggtcaag
     4921 atataataga tgaattactt agtggtgtat atgaagatac tcagtggtta ataagacttg
     4981 ttgaaaattt acttagtatg accaagatag atgaagggaa attggaagtt aaaaaacatt
     5041 ctgaactggt tgaggaaatt atatcagaag cgttacaaaa aataaaaaaa agaattgaaa
     5101 atgcacttat agatattaat ataccagaac agatattatt tgttcctatg gatgcaaaat
     5161 taatagaaca agttttaatt aatttaatag ataattcttt gaaatattca aaggaagatt
     5221 gtaagataga gataaatgta tatgagaaag atgattatgt ttggtttgaa gtatctgata
     5281 atggtcctgg tatatcaaag gaacttaaaa agcatatctt tgacagattt tttactggag
     5341 aagaaggagc taaagattcc agaaagggtg ttggacttgg attatcaatt tgtaaatcta
     5401 taattcaagc tcataaaggt gaaattatgg tagaaaacaa taaagataag ggttcaactt
     5461 ttaagtttag tttgcctaag gaaaatgaat agttttaaat tttaaaatta gttttggagg
     5521 aagaaatgag taaaaaactt atattgttag tagaggatga taagacaata agaaaattta
     5581 tatctactgc acttttaaca caggattatg atgtcaagga ggctataact ggaaaagaag
     5641 gtatatctat tgctgtctca tattctccag atgttgttct tttagattta ggcttagaag
     5701 atatggatgg aatagaggta ataaaagcta tcagacaatt ttcaaatata ccaataattg
     5761 tagtttcagc tagagagcaa gatagagaca aggtagaagt atttgatgca ggggcagatg
     5821 attacttaac aaaaccattc tccatagtag agttattagc aagagtaaga gtagcattta
     5881 gacattcaca agtagaagct caacaaaaag atgatgttaa atctacgttt gaagtagata
     5941 aattattgat tgattttgat aaaagaaaag ttattgttga tgatgttgaa gtccatttga
     6001 cacctattga atataatatt ctttcattac ttgcaaagca tcatggaaaa gtgcttactc
     6061 ataattttat aataaaagaa atatggggta gtgttatagg gaatgagact aagtctttaa
     6121 gggtctttat ggcaacgtta agacgaaaaa tagaaaaaca acctgcaaac ccaagatata
     6181 taattacaga agttggtgta ggatacagat taaatgatga atagttgtaa tatattaatt
     6241 attagattat gtgcttatta atatatatta aaattatata gtttagttaa aataagaatg
     6301 aagtgtatgt gcttgttgtc agtttagcac atgcatttta ttttttagct tttttagtat
     6361 caataatttt ttagtatcaa taatatgaaa tatgttttaa aataagtata tagtgtaatc
     6421 tgtaataaaa actttg
//
LOCUS       DAFKMX010000196         6414 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFKMX010000196 DAFKMX010000000
VERSION     DAFKMX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.199"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            295..2382
                     /locus_tag="LZB08_003237"
     CDS             295..2382
                     /locus_tag="LZB08_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBM7316704.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGIYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNTEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2397..2684
                     /locus_tag="LZB08_003238"
     CDS             2397..2684
                     /locus_tag="LZB08_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBM7316705.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2711..3982
                     /locus_tag="LZB08_003239"
     CDS             2711..3982
                     /locus_tag="LZB08_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBM7316706.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4040..4639
                     /locus_tag="LZB08_003240"
     CDS             4040..4639
                     /locus_tag="LZB08_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBM7316707.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4644..5549
                     /gene="dapA"
                     /locus_tag="LZB08_003241"
     CDS             4644..5549
                     /gene="dapA"
                     /locus_tag="LZB08_003241"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBM7316708.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5655..6287
                     /locus_tag="LZB08_003242"
     CDS             5655..6287
                     /locus_tag="LZB08_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBM7316709.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
BASE COUNT         2358 a          767 c         1165 g         2124 t
ORIGIN      
        1 aattaatgtg ctttttaatt gtagtaattt atttataatc aatatgattt ttattattta
       61 aatcaatttt tcttgagttt ctttgtctaa agaaactttt tttatttata aaatctacag
      121 ttatattata gtatctattt tagatgagtt taatttttgt atcaatctaa aacaaaagtt
      181 atacttaaat gaaattgaaa acgataaaat tctcaaataa aataagtatt gtggaggtga
      241 tgaaagaatc caatgataat atgaagacta atcttaataa gaaagtaggg gttaatggta
      301 gtggacaaag aaattgtatc aattctccag attttattaa atagcacatt gattacaact
      361 aatggattac aagaggagtc taattcatca aagagacaaa ttacttatag aataaataaa
      421 atcaatgaca tgcttaaagt aaaaaatgta ccacttatat gtttaagagc cgacaaagat
      481 attatcgtta gaaaagaaac aaaaaaagca attaaagaaa ttttagaaaa aaactattct
      541 aagaatactt attattttag taagacagaa agacttttat atatgtattt aatgcttttt
      601 attaacatgg aggatatttc aattaatcat tttataaatt ctataaaagt aagtaggtct
      661 actgtaaatc ttgatttcaa ggatttgata ccagagcttg aaaagaagaa cattaaagtt
      721 aagaacaatc gtataaatgg atattacata gttggcaatg agatggaaat taggagagta
      781 cttattaaaa acattataga aactttgtct aatgagaaaa gttcaagagt atttgatatt
      841 tttattaaag aatataattt agatagtttt gaagaggcta aaaaaattat attaaagcta
      901 ataaaaaaat atgaaattac ttttgttgaa gatagattaa tagaatttat atatatattt
      961 atctttctaa aagcaaggat gtattcaact agaatagtta ctcaggaaaa tgtagatatt
     1021 cctaatattt cagttatgag ttcaatgaag gagtataaat ttgctaagga attattagaa
     1081 gtatatggga gtgaagataa gataaagcct cacaatataa tgtatattag tgcttggatt
     1141 ttaggtatat cagtaggaaa ttatgaagaa gatacagaag ataaggatgt aatttcaaaa
     1201 attgtaaata aaatgatgac aaagtttgga tatttaagcg gaatttacta tataagccaa
     1261 gaaaaaatat ttaaacaatt atattctcat tttagacctg cctactatag acttttattt
     1321 aaattaccta tttataatcc gatttgtgaa aaagtaaaag aagaatataa attagtttat
     1381 agaattgtaa gtaatgcaat gaaagagttt tgcggattat ttggagaaga aattcaggaa
     1441 gaagagttag catatcttac aatgcacttt gcaacacttt tttcaaataa aaaagaattt
     1501 gatggtttta gaaaaagaat agcattaata gtttgttcaa atggcgttgg tagttcagct
     1561 attttacatg cagaacttac aaacttattt cctgacttac actttatacc aatgtcggaa
     1621 tcagttggat tagaaaatat aagtgaacct gtagatattg tatttactac taattataac
     1681 acagagggtt taaaaacaga tgtaccagta attaaagtaa gccctgttat gaccacaaaa
     1741 gaaaaatata agattacacg tgaagtgtat attcaattag gtgatatttt tttaagacaa
     1801 cctaaaatag atgaggttat ggatataata aaaaaatatg caaatgtaac ctctgaaagt
     1861 attctttcaa gtgagctatt agcttatttt acacagatag agaattttac atctaaggaa
     1921 ggagaggggc ctatgctaag tgagattact aatgaaacac tagtaaaaat aaaggtaaaa
     1981 gctaagaatt gggaggaagc tattagacag tcagcttctg tattagttga aaataacaaa
     2041 gtaactgaag ggtatgtaga tgctatgatt aactcagcaa aagcatctgg accatacata
     2101 gtaattacaa aacatgttgc acttccacat gctagaccag aagctgggtc aaaagagatt
     2161 gcaataggca ttgctacttt agatagtcct attgagtttg ggaataacga aaacgaccct
     2221 gtaaaatatg tattttgtct aagtgcaata gacaataata gccatttacg tgctatgtct
     2281 gagctagttg aactattgga agaagatgag ttttttaagg ttttagatag tgctaaagaa
     2341 gctaaagaaa ttattgattt tataaaaaca cacgaattat aaaggaggat ttaattatgt
     2401 ataaaatttt aatcgcatgt agagcaggag ttggctcaag tttaatgtta aaaataaaga
     2461 ctcaacaaat tattaaggaa aataactttc caattgaagt agaacatggt tcattggatt
     2521 cgctaaatgg attcaatgga gatgctgtag aaacattaat tgatgttgct gaagaattaa
     2581 aaaataaaaa tttgaagttt gatataattg ggattcacaa tattgttgac cgtaatgaaa
     2641 taaagacagc actagaaaaa ttccttgctt caaaagaggg ataaatttaa gatgataagg
     2701 aggaatattt atggagttta ttattagttt gctgagtaca cctgctgttt tgcttggcgt
     2761 agttgcatgt ataggattga ttcttcaaaa gaaatcagct gttgaagtat ttacaggctc
     2821 ttcaaaaaca cttatcggtt ttttgatatt tggtattggt gcctctgcta tgacaggagc
     2881 acttcaaaat ttcaataaac tgttccaaca tggctttgga attactggtg ttatagcttc
     2941 accagaagca gctacagcac ttgcacaagg tacatatggt tttgcagttt catgtacatt
     3001 aattcttgga ttcatattaa atttagtatt tgcacgtatt acaaagttta agaatatttt
     3061 ctttacaaca ggtcatagtt tgttcttttc ttgtgtatta gttttaatta tgaaagcaca
     3121 tggatttgat aatacaatca ctattttaat aggaggaact atcttagggt ttatgtctgc
     3181 tgcactacct caattctgtc aaccatttat gcgtgaatta acaggtggcg acgaacaagc
     3241 tattggtcac tttaacatga ttggttatgg tttatcaggt tatattgggc gtttattttc
     3301 aaaacataaa gatgatacaa cagagactat agaatttcct aagtggttat caatattcag
     3361 agacttctta atgggtctat ccatagttat gttaatctta ttttatattg caacattaaa
     3421 agctggtcaa gcatttactc aagaaattgc tggttcaaca cattggttgg tttttccgct
     3481 tatagaggca tttacatttg tggcaggtat gtcaatattg atgtcaggtg ttcgtatgtt
     3541 cttagctgaa ataactgcag catttgtaat catttcagaa aagtatattc caggttcacg
     3601 tcctgcgcta gatgttccaa ccgtatttcc atatgcacca aatgcagtta ttattgggtt
     3661 tatatcagca tatgctgctg gtctattagc aattgctatt atggcatcat ttcctagtat
     3721 atttcctgta gtaattattc cagctgctca catatgtttc ttctcaggag gtacagctgc
     3781 aatatttggt aatacttcag gaggatggag aggggctatt gctgggtcat ttgttgtggg
     3841 gttattactt gcatttttac cagttatact atatccagtt tatggcactc ttggtataga
     3901 aggagcaact ttccctaata ttgattacaa tgtagttggt tcgatattac ataatatttt
     3961 acaatttata aaacctattt aaactaaatt atataaaagt atagtcatag ttactatgat
     4021 ttcaaggagg atacatctta tggaaaatac attatctgaa tttttagata atatatctaa
     4081 tgaattgtca gaatttattg cctcaataga tgagacatct ataaataaag catgcgaatt
     4141 aattcttgaa gctgaaaaaa atcacggacg tgtacatgtg actggtattg gcaaaccagg
     4201 acatgtatcg ggatatattt catcattgct ttcatctact ggaacgtcag catatatatt
     4261 gcatggtaca gaagcagtac atgggtcaag tggtcaagta gttgaaggag atgttgttat
     4321 tgcaatatca aatagtggtg aaactcaaga gttaaaagca actcttaaga ctctaaaagt
     4381 aaatggagca aagataattg gtgtaagtgg taatgagagt tcatttttaa aaaatatttc
     4441 tgatatattc ctgtttgcag gtgttaagca ggaaggagat tgtcttaata aagctcctcg
     4501 tgcttcaatt ttagctgaaa caataatatt acaatcatta agtgttgtat tacaatatgc
     4561 taaaggtctt aatacacagc agtatttgaa gtggcatcca gcaggaagtc ttggaaagtc
     4621 tattagagag ggaatataat tttatgaaat ataaagggat tatatgtgca atgataacac
     4681 catttgatga aaatcaaaat attaattcac aagcaacatg ccaattaatt gattatttga
     4741 ttgaaaaggg aatttatgga ttatttattt tgggcactaa tggtgaatgt catgtattaa
     4801 cagatgatga aaaagttgaa tttgcaaaga ttgttattaa tcatacaaat aatcgtgtac
     4861 ctgtttttgt tggaacaggg ggtaattcaa ctcgtgaagt tgtagattta tctaagagaa
     4921 tggaagcgat tggagcaagt gcattatcta ttattacacc atactttgtt acaccaacac
     4981 aacaggaatt aatcttacat tacaaaacta ttgctaattc agttaattta cctattataa
     5041 tgtataatat gccaggtaag actggaatta atattgaacc agagtcagta cgtgaattat
     5101 caaagataaa gaatattgtt ggaattaagg atagtagtgg taaacttgac aacatgaaag
     5161 cttatattga agcaacgaag aatgagaatt ttagtgtgtt ttcaggctca gattcactta
     5221 ttctggatag tctaaaagct ggtagtcaag gagctgttgc agcaacaaca aacttcctca
     5281 cagagattgg tatctctatc tatgaaaact ttatgaaagg ggatttggaa gaagctcaaa
     5341 aagctcaaag tagtatagaa gaacttcgac gtatactgaa acttggaaca attccatctg
     5401 ttatgaagaa aactattgtc ttgaatggaa ttaatgttgg aactgcacga cttcctgtaa
     5461 ctgaaccaac tggggaagta cttgaagaaa ttaaacgagt agttgaaaat tataggacaa
     5521 tacttaataa aagtagtgag aatagataat ataaataggg aaattacact ggtatataat
     5581 taatatatat attataaaac atgactaaaa ttaatgaata agtaaagtgg tttgatgagt
     5641 atagaggtga taatatgaaa aagatgaata taaccaaaag aatgtcagaa tgtggggctc
     5701 ttgcaattgt aagagaagaa aatcttaacc gtgcatgtga aattgctgaa ggatgtataa
     5761 aaggtggtat aacagttata gaaatgagtt ataccctaaa taatgctgga gaaataattc
     5821 aagggcttaa taaaaagtat ggtgaaacac tttgtgtggg agcaggaaca gttttggata
     5881 gtgaaacagc tcgtcatgct atattacatg gagctcaatt tattattgca ccaaattaca
     5941 atgaaggtgt agcgaagatt tgcaatagat atcagattcc ttatgctcca ggatgtacat
     6001 cattgactga ggcagtagac gcactaagtt taggagcaac ttttataaaa gctttcccaa
     6061 tatcagattt ctatggacct aaactagtaa aggtatttaa gacaccaata cctgatatgc
     6121 cagttttagc aagtggaggg cttaatattg aaaacctaca tatatggtta gaaaatggag
     6181 ttgatgtatg tggatttggt ggtttattaa ctaaaggttc tgttgaagat attgctaaaa
     6241 atgctaacaa gattcgtgaa atcattaaaa atgtaagaaa aatttaacat agggctgtat
     6301 catagtatct tataattact atgatacagc ctttttaata ttattcaaat ttaaaacagt
     6361 ttacatttct attatattac atataatttc agtaatttta tcttaatttt tcta
//
LOCUS       DAFKMX010000197         6387 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFKMX010000197 DAFKMX010000000
VERSION     DAFKMX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.200"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            14..1897
                     /locus_tag="LZB08_003243"
     CDS             14..1897
                     /locus_tag="LZB08_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBM7316710.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKESILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGDFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2121..2801
                     /gene="pgmB"
                     /locus_tag="LZB08_003244"
     CDS             2121..2801
                     /gene="pgmB"
                     /locus_tag="LZB08_003244"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBM7316711.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2798..5083
                     /locus_tag="LZB08_003245"
     CDS             2798..5083
                     /locus_tag="LZB08_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBM7316712.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            5309..6202
                     /locus_tag="LZB08_003246"
     CDS             5309..6202
                     /locus_tag="LZB08_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBM7316713.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         2656 a          573 c         1150 g         2008 t
ORIGIN      
        1 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaataaa
       61 aggaacaatt caaaatagtt taacaaaaga atctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtgatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggt taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1921 tttcatgtta aattattata cagtttaaac aagtctttat aaatgataaa aaatatggtc
     1981 tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag tagataattt
     2041 tataaataat atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct
     2101 tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta
     2161 cagatactgc atattatcac tatatggctt ggagaaaact agctcataaa gttgggatag
     2221 atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca
     2281 ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg
     2341 ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca aatgatattt
     2401 tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat
     2461 cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg
     2521 attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta
     2581 tgtcagcgaa agggcttaat gtaaatccac aaaattgcat aggaatagag gatgcaagtg
     2641 caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa
     2701 acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac
     2761 aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt
     2821 agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc
     2881 cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag
     2941 tggagataca cacaaggggt cttatatagc tggagtatgg tttccagaca aaactcgtgt
     3001 tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaatta
     3061 tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga
     3121 agatttttat agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa
     3181 tataaatgat aaaaaatttg aagttagggt tgctaggttt ttaagtatat ctgttaagga
     3241 acttgcagta attaaataca gtataaaatc attaaattct aatactaaaa taaaatttgt
     3301 accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa
     3361 tggaatttct tcaagttctt tagaatataa aggtagtatt ctttctcgaa caaaggagaa
     3421 tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga
     3481 tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga
     3541 tgttttaaaa aatgaggttg caagtataga aaaatatgta tctataacct caactagaga
     3601 ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatagag aactatctaa
     3661 aagttatgat gatatattaa atgaacatat aaagttatgg aataaaagat gggaaagtgc
     3721 agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca
     3781 actattttca acatattatg gagaagattc gcgattaaat ataggtccaa agggatttac
     3841 tggtgaaaag tatggaggag ctacttattg ggatacagaa gcgttttgtg ttccagtata
     3901 tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt
     3961 agaacaggca aaggataatg caaaaaaact agggcttaaa ggtgctttat atccaatggt
     4021 aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg
     4081 aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg actataaata
     4141 tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt
     4201 tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta
     4261 tgaaaacaat atcaataata attggtatac taattatata gctaaatggt gtttagatta
     4321 tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa
     4381 tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt atctgccgta
     4441 tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga
     4501 agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat
     4561 tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa
     4621 tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtcca
     4681 tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga
     4741 aaaggcttat gaattatatt caagaacagc taggcttgat ttagataatt acaataatga
     4801 tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg
     4861 atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt tgccaaaaga
     4921 gtggaaaaat tattctttca atataaatta tagagataat attattaatg tagatgtatg
     4981 tagtgattta attaaaatag aaaatttaaa aggtaaacca ataaaaatta ttgtatatgg
     5041 gaaaaaatat atattagaaa aatgtataaa aattgataaa tagatttaat aatattttta
     5101 ttatataaat agttaaaata agcttatcta attatttatt tattttaact gttttgtgtg
     5161 atagaaaact atatattata acaatagtag tgcttttaat taaatatatt tcataaatct
     5221 tcataaaatc ttcaaaattt cgtaaagaat atgtcacaaa agtaaggtat attatattca
     5281 gcagatagta taagggagga caatatttat gaagaataaa aaattcattg ttataatact
     5341 gataatatca attttaatag gtgtattagt gaaggaatat tcatctagag agataaaaaa
     5401 ggatgatata aatgtcagta agtatataaa atatgctgat ttagcaagta aaaataatgc
     5461 acaagttaac tggaagtatg tagcttccat tgttgctgtg ttaaataaaa ataacttaaa
     5521 aaacgttaag gattcacaga tacaagaagt atcagattta tttgttaaga atttttctaa
     5581 aaataataaa ataaataagt taagtgatat tttggatgag ttagaattta gtaatagaca
     5641 aaaaagattg gttgataatt atatagacaa tcttaaggat tatggtataa aaccagaaag
     5701 attgaagtct gatacaaaat atatgaagtt tattgatgaa attaaaacag aagcaataca
     5761 aaattataag gattataaaa tacttccatc tataactatt gcacaagcta taatagaatc
     5821 tagctggggt aagtcaactt tggcaaaaca atataacaat ctatttggta taaaggctga
     5881 cgcttattgg aaaggtaagt cagtgacttt agaaacaaaa gaacatttag atacaattat
     5941 agatgataaa tttagaatat atgatgataa aaatgaatct ataaaagacc atgcaaaatt
     6001 tttagccaca aataaaaggt ataaaaacaa tggtgtattt gatgcaaaaa cgtatatata
     6061 tcaagctaaa gcattagaaa aggcaggtta tagtactgcg aaggatgaaa atggcaatag
     6121 tatatatgca gctcgtctaa tagaattaat tcaacagtat aatcttcaat taatagacag
     6181 tgaaattcaa agtgaaatgt aactttgatt aaatttttat tgataaggct atgattttta
     6241 aaatcatagc cttattttga catgctttta cttgtaatta ttaaatcgat atcattatct
     6301 ttcaaaattt actttatact gtaaatcaaa ttatttaagt taaaacttgg aattatttat
     6361 aaaaggatta tttactttat ctaaatt
//
LOCUS       DAFKMX010000198         6372 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFKMX010000198 DAFKMX010000000
VERSION     DAFKMX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.201"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            257..1732
                     /locus_tag="LZB08_003247"
     CDS             257..1732
                     /locus_tag="LZB08_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBM7316714.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1819..2232
                     /gene="speD"
                     /locus_tag="LZB08_003248"
     CDS             1819..2232
                     /gene="speD"
                     /locus_tag="LZB08_003248"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBM7316715.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2262..3113
                     /gene="speE"
                     /locus_tag="LZB08_003249"
     CDS             2262..3113
                     /gene="speE"
                     /locus_tag="LZB08_003249"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBM7316716.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3103..3981
                     /gene="speB"
                     /locus_tag="LZB08_003250"
     CDS             3103..3981
                     /gene="speB"
                     /locus_tag="LZB08_003250"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBM7316717.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4161..4361)
                     /locus_tag="LZB08_003251"
     CDS             complement(4161..4361)
                     /locus_tag="LZB08_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBM7316718.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4828..6318
                     /locus_tag="LZB08_003252"
     CDS             4828..6318
                     /locus_tag="LZB08_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7316719.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         2551 a          689 c         1130 g         2002 t
ORIGIN      
        1 taataaaaaa taagaaagtt tataaatact aagttttaaa aaaattaata taaacaacaa
       61 aaaattaaaa agtttataaa tattgatata gctaatgtat atgtttttga gtaacttcaa
      121 aaaaacactt gtaaaaatgc tgggatgtta atataataaa tatcatgagt ttaataaaag
      181 taaaaataaa aaatagaaat aataaataat aaatattaaa aatatttgaa tatagagagg
      241 agatgaattt gaaatcatgg atagcgagtt tgcaaatgca gttgaattaa gctatgagtg
      301 tgctccatta ttagagtcgt taaaggaata ctcagagaaa gatatagctt gttttgatgt
      361 gcctggacat gtaaaaaata gaggtgtagc tatattaaat aaatatctag gtgaaagtct
      421 catgaaaatg gatataaatt catcaccaac tatggataat gtatctgcgc caaatgggat
      481 tataaaaaat gcacaagatt tgctagcaca agcatatatg gctgatgagg cattctttat
      541 aacgaatggc acaactcaag caatacatgc aatgattttg agtgttataa aaccaggtga
      601 aaaagtatta cttccaagaa atatacataa atcagtaata aatgcgttaa tactttgtgg
      661 aggaatacca atatttatac aaccagaatt tgatgaaaaa ctaggtataa gtttaaatat
      721 tactcttgaa aaagttagaa cagagataga aaaagattgt aatataaaag cattattttt
      781 ccttaatcca acttactatg gagtttgtgc agaccttgag agtattatag aactatgcca
      841 taaaaataat gttttagtgt tagttgatga agctcatggt gcacattttc catttcattt
      901 agatttacct ccttctgcaa taagtttggg agctgacatg gtagctgtaa gtatacataa
      961 aactggagga gcattgacac aatcttcagc actcttattg aatagagata atgtaagttt
     1021 tgaaaaggtt cttcaatcaa tcaatatgct acaatcaaca tcagcatctt atttgcttat
     1081 ggcaagtata gatggtgcca gagttaatct agttgagaac ggtgaaaagc aactgtcaaa
     1141 agcattaaat ctatctaggt atgcaaaatc taaattaaac aaaattgatg gaataaaagt
     1201 tttatctaca gaaatattga aacaaaaagg tgttaaattt atagatgaaa caaaattgtg
     1261 tataaatgta aaagaattaa atttgactgg atttgaagta tacgatttat tatataaaaa
     1321 tttttcaatt caagtagaat tgggagattc atataatata ctggcacttg tgtccattgg
     1381 aacaaataaa tcagatatag atagattagt aaaagcactt tctataattg caaagattta
     1441 tagaaaagaa tcaacattaa atgaatttaa tatggttcaa ataaatccaa ttattaaact
     1501 taatccaaga gaagcttttt atgcaccaaa ggaaagtgta gaaatcaatt catgtataga
     1561 tagaatttgt ggagaatcaa taatggcata tccaccaggt ataccgatta ttgctccagg
     1621 agaattgata acagaagaaa ttatggagta tattattttc ttaaaaaata gcaatgcata
     1681 tcttacagat gtacaagata aaaacttaga tagaatatta gtaataaaat aaattacttt
     1741 attttgcaag ggcaagtatt tgttaagtag agtttatcaa atagaactta ttaaaagtat
     1801 aaaggagttg gggttgaaat gaaatttgaa acactaggaa gacatatact agtagaatac
     1861 tataattgtg atgaaaacgt acttaaagac ccacgtctta tagaaaattt tatgaatgat
     1921 tcagcattaa atgcaaaagc gacaattgtg gattctgtat tccaccattt taatccttgg
     1981 ggggtttcag gagctgttat aattgctgaa tctcatttga caatacatac atggcctgaa
     2041 tatggttatg cagcagcgga tttttttact tgtggtgata tagacccatg gaaaagtttt
     2101 gaattattag agcaactttt aaagtctgaa agaagcgagt ctacagaaat tccaagaggc
     2161 ttaactacaa aaattaaaaa atattcaaaa aaagatttag gtaagattac tcataaacca
     2221 gaagcagtat aaaacataca agagaaagtg aggattatta tatggaactt tggtatacag
     2281 aagaatggac agaaaatgta agattttcta taaaagtaaa taaacatcta tttgaaggaa
     2341 agtctcaatt tcaaaggata gatgtatttg atagtgatga atttggaaaa tttctaacaa
     2401 tagatggatt aatgatggta acatataaag atgaatttat ataccatgag atgataactc
     2461 atgttcctat ggctacaaat ttaaacataa agaaagtatt agtcataggt ggaggtgatg
     2521 gaggtacagt aagagaatta agccgttatc ctcaaataga aaagattgac atggttgaaa
     2581 tagataaaat ggttgtagat gtatctaagg agtatatgga tatatgctca tgtaaattag
     2641 atgataaaag agtgagttta tattttgaag atggtgttaa ttttgttaag tgtgcacatg
     2701 ataaatcata tgacctgata atagtagatt caactgaccc aataggacca ggcgaagggc
     2761 ttttctcaac tgatttttat aaggattgtt atagaatact gactgatgat ggaattttag
     2821 taaatcaaag tgaaagtccg tattttgact ttaatgctaa agagatgaaa agagctaata
     2881 aaaagttaaa acagatattt ccaatatcag aagtttatca agcacatata ccaacatatc
     2941 catctggtca ctggttgttt ggatttgctt caaaaaaact taatccagta aaaaatcaag
     3001 atagaaatgg atgggagaaa ttaagtttaa aaactaaata ttataatagt gatatacatt
     3061 taggttcgtt tatgttacct caatacgtga aggagatgct agatgaagaa taatttctat
     3121 cataggaaca cttttatgag tatgaacaaa aattatgaag aatctaatct tatagtattt
     3181 ggtgttggat ttgatggaac tacttctaac agacctgggg ctagatttgc aagtagctct
     3241 atgagaaaag aattttacgg tcttgagaca tatagtcctt ttttagattt ggatttagag
     3301 gattataata tatgtgatta tggagattta gaaattagtg ttggaagtac agaacaagtc
     3361 ttaaaagaaa tctatcaaga gacatataag attgttagag attcaaaggt accctttatg
     3421 attggaggag agcatttagt tacattacca gcctttaaag cagtacatga aaagtacaat
     3481 gatatatatg taattcattt tgatgcccat actgacttga gggaagaata taataatagt
     3541 aaaaattctc atgcaacagt aattaaaaga atatgggata ttgtaggtga taataaaata
     3601 tttcaatttg gtataaggtc tgggacaaaa gaagaattta aatttgctac agaagaaaaa
     3661 cacacataca tggaaatagg aggaatagat acgtttgaaa atatagttaa tatgctaaat
     3721 ggaaagaata tttatctaac tatagattta gatgtattgg atgcagctgt gtttccagga
     3781 acaggtacac cagaacctgg tggtgtaaat tatagagagt ttcaagagat ttttaagatt
     3841 ataaaaaact ctaatataaa tatagttggt tgtgacattg tagagttaag ccctgattac
     3901 gatacaacag gtgtatcgac agttatagct tgtaaaatct taagagagtt atgcttaata
     3961 atatctgata aaattaaata gagtatttta tataaatagt atatattata atataagaag
     4021 ttattttaaa aaagttagtt taagtttgac gcttttctaa agtaacttct tatttttgta
     4081 attaaaaata aatataataa acaacaaaaa agagccctcg atagctcctt ttctaaaata
     4141 tatatattta aattaaataa ttatattcta tttacgtttt cagcttgagg tcctctatta
     4201 ccttgagtta tatcaaagct aaccttttcg ccttcttcta aagttttgta tccatcattt
     4261 tgtatagctg agaaatgtac gaaaacatca tcttctccct ctacagatat gaacccaaat
     4321 cctttttcat tgttaaacca ttttactatt ccgtttttca ttataagaaa acctccgcat
     4381 tattaaaaaa tacaaactaa aaaaataagt ctgttctatt aatataacat aaattttaaa
     4441 taaaagcaaa taaaattttt aaaaaaatta attactttta gaatttaata aaaattgtta
     4501 aattatacag tgataagtat tgtaaaattt gacgaaaatg taaatgtaga taataaattt
     4561 aatatcaaaa aatgtaacaa aaaggtatgt aagtaaaaag taaaattaaa atatataata
     4621 aaaaatatta tatattagat tgaaaagtaa aaataaaaat agtgaaattt cagaaatatc
     4681 aataatatta tataataaaa caaaaaaaca ctatatatat tcgataaaat aaaatttaaa
     4741 catataatat tttatgtata tattaataaa aggacatatt gtgttataat ggtatcgtat
     4801 aaaatcattt acgggggtgg tattaaaatg tttcaatttg aaacacaaat acacaaatta
     4861 agacatgatg ttttaactgc aatagctaga cttggaaaag aagataacct taccagagaa
     4921 gcaatggata atattcagta tgaaataata aaaggtgaga aacctacata cagatgctgt
     4981 gtttataaag aaagagctat aatatctgag agagtaaatg taagtatggg gttacaacca
     5041 ggtcaagata agatagattt agatacaatg gatatagaag aagatgaaca aattatatat
     5101 attttggaag ctgcatgtga cagttgtcca ataaatgaat ttacagtaac agatatttgt
     5161 agaggatgtc tagcacatag atgtaaagaa gcttgtaaat ttggagctat aacacatgtc
     5221 ggaggaatgg cctatataaa tcatgaatta tgtaaggctt gtggaatgtg taaaaaagct
     5281 tgtcaatatg atgccatctc agaggttgtt agaccatgta agagtgtttg tccaactaat
     5341 gccttaggat ttgatagaga aaatatgaaa gctatgatac atgaggataa atgtttaaac
     5401 tgtggagcat gtatgtcagc atgtccattt ggagcaatat ctgataagag tttgatagct
     5461 cctgtagcta gaaagttagt tcaaaaagag aaaatgtatg cggtggtagc tccagctata
     5521 acaggtcaag ttgaagcaaa tgttacatat ggtcaaataa aaaatgctat aaaatcacta
     5581 ggatttgttg atgtattaga agcagcttgt ggagcagatg cagttacagt tcatgaaagc
     5641 ttagaatttg ctgaaagaat ggaaaaggga gaagctttta tgacaaattc ttgttgtcca
     5701 ggatttgttg gttatataga aaaaacatac ccaaatcatg tagggaaaat atcttctaca
     5761 gtatctccta tgatagctag tggtagattt ataaaaagtc atgaaccaga tgctaaagtt
     5821 gtattcatag gaccatgtac agccaaaaag agtgaggcag ctaagaaaga attaaaagga
     5881 gctgttgatt atgtacttac ttttgaagaa ataatggcat tatttgaggc atttgatata
     5941 gacttggcta agtgttcaaa tgaagatata gatgatgcat caatttttgg aagaggtttt
     6001 ggagctgctg gtggtcttac aaaagctata gaaaattatc ttgctgaaaa aggtattggt
     6061 gttaattttg aacctgtaaa gatttctggt tcaagagaaa tcaagaaaac tatgactatg
     6121 gctaatttag gaaagctaga aggtaatttt atagaaggaa tgatgtgtga aggtggttgt
     6181 ataaatggtt ctggtaaatt cttatcaggt gcaaaggtta aagccacttt tgatagaaaa
     6241 aattctcaat caactaagaa aagtgtctta gcaaatacaa agataaaaga ttttagtaat
     6301 ataaatttag aaaaataaag ataatacaaa gcaacaaaaa tacatattgt taatagagtt
     6361 gttctaagtt ga
//
LOCUS       DAFKMX010000199         6296 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFKMX010000199 DAFKMX010000000
VERSION     DAFKMX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.202"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            161..679
                     /locus_tag="LZB08_003253"
                     /pseudo
     CDS             161..679
                     /locus_tag="LZB08_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1111..1365
                     /locus_tag="LZB08_003254"
     CDS             1111..1365
                     /locus_tag="LZB08_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316720.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1401..1724
                     /locus_tag="LZB08_003255"
     CDS             1401..1724
                     /locus_tag="LZB08_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316721.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1998..2150
                     /locus_tag="LZB08_003256"
     CDS             1998..2150
                     /locus_tag="LZB08_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316722.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2344..3266
                     /locus_tag="LZB08_003257"
                     /pseudo
     CDS             2344..3266
                     /locus_tag="LZB08_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     repeat_region   3533..4419
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3533..3561
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4610..4762
                     /locus_tag="LZB08_003258"
     CDS             4610..4762
                     /locus_tag="LZB08_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316723.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4883..5248
                     /locus_tag="LZB08_003259"
     CDS             4883..5248
                     /locus_tag="LZB08_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBM7316724.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIRL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQGWISDEV
                     LPSIRQTGAYINNNAIPKS"
     gene            5245..5697
                     /locus_tag="LZB08_003260"
     CDS             5245..5697
                     /locus_tag="LZB08_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316725.1"
                     /translation="MREKASEIEKLQLVYNSTSMLKELLDDAGFDNKSKLLTAKTLYK
                     KAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVW
                     ETNGSWTGTVNKYTKSVIDKVRTWIEENNRPTKIADEKKNYHVFYKIE"
     gene            5749..5910
                     /locus_tag="LZB08_003261"
     CDS             5749..5910
                     /locus_tag="LZB08_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316726.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
BASE COUNT         2598 a          593 c         1089 g         2016 t
ORIGIN      
        1 tttaatagaa tatgtatagt atatagtaga tagaatgtat aaaaataaaa actgttcaat
       61 aagaattaat atacattaat tcagctgaat ccaatcaaaa tacttaaata aaagtaatct
      121 gaaaataaca taattcaaat caatttattc aaataaacct caatttatct caaatcttaa
      181 gaagtcatta aacaaacttg ctaaattaca aagagaatta tcaagaaaaa caatcggtag
      241 tttaaataga aataaagcaa gattaaaagt tgcaagactt caagaacata tagctaatca
      301 aagaaatgat tttctacaaa aactatctac taaattaata aaagagaacg atattatttg
      361 catagaagat ttacaagtaa agaatatgat taggaatcgt aaactatctc gctcaatttc
      421 tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt ggcatggtag
      481 acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata aatgtgggta
      541 taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttatccta gttgtaatga
      601 aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat
      661 aacaattcaa aataaataac gtatgtaaga accgtaggaa ctacggggat agcctgtgga
      721 gaattagtaa gacatatgtt agtatgcaaa attctgtgaa gcaggaaacc tgtgacttta
      781 gtcatgggag gttcaggcta gtattactca ttattgtatt caaaagctaa agtggaaacc
      841 tagtgaatat atgaatttag aagttaatta gagagcatta gcagccgact caatacttat
      901 taaaattgaa gatgaagaag aaacaatgaa agaagctgaa agagagaaaa agagaggtag
      961 aagaaggtag aatttaaagt taaaatagtt acattgctaa tatatgaaaa tgttacaaat
     1021 ttggaaatag atggatatat atgttgtata gtataatgta gataatatat caccaagtaa
     1081 aaaacaacaa ataaatctaa attatgaggg gtggaaaata aagatattaa acttacaata
     1141 tgtatatttg cttttgtaat tggaataata gtgttatttt gctgtatagg tctaggtcaa
     1201 tttaatatgg attgtattat aaaacaaaat ggtgggtcaa tagctaccga acaatacaac
     1261 atatatttag aacaatctat ttcacaatat agaaattttg gttctatact ggttttacta
     1321 ggaggaatag gaatactgtt tgataaaagt gtcagaaaat actaaaaaag tatattttta
     1381 tttaggatgt gatgaatgat atggcaagag atgtatttag tgtaaagatg acaccactag
     1441 aagcacagaa aatggtagaa aaaaatttgg aagcagattt agtttctgtt gatatttata
     1501 acctagaaaa taataagtct ataatagtta caatctttga aaaatattat gcaagaagca
     1561 atagtgatgc aggtttggta gtgatttgtg aaaatactac aggaaaaaca cttgttaaaa
     1621 ttacctcaac tggtagtgca attggactat ttcagattga ttggggaaca ggtaataatt
     1681 tgattaaaag agtaaaaaaa atacttattg attatattat ttaattattt tatgtagata
     1741 ttcttaggtt ggtatggaat ttgtactaac ctattaagta atcaataaaa ctaaataaac
     1801 atagaaagca cttacaaata tgtaggtgct ttttattgaa aaaatagaaa ataaaataaa
     1861 aagtaagata taggaaaaat atgtaagaat tatatgttat aataattgta gcaagaagat
     1921 gtaatctaca atttatagag tggagttcat gtattaaaaa attatcctcc caacgataag
     1981 aagggaggtg aatatacatg gataattttt tatttaatgt gttagctagt gtaacagcta
     2041 gttacatagt ttatttgatt agcaagttaa tcaaaaaagt aaaaagccac tctaccgcaa
     2101 agagtgactt aaagattgat ataaatttta aatatcatcg taaaaaataa ctttcagtta
     2161 tagaacttca ctctaccgca aatagattgt agttcttctt gtttttatta taccacaaat
     2221 tagaaaaaat attgcatata atatttttta tagtcaataa aaatatgaaa ttttttatac
     2281 acaatgatga tgaatctaac tagatgaata aatttaattg taaataagaa agtacttact
     2341 taaatggtag gtgcttttgt tttgccaaat tatgctataa tattgagtga gatgagtaat
     2401 ttgtggggga attttataat gtggatatta gtaccaataa taacaatagt attacttata
     2461 atagctgttt cttctatgca atatatttta gttatgatag cttttttgtt gattatatat
     2521 tcttttatag aaaagaaaat agttatgggc cttgtgtcag ttcttttttt cacttattca
     2581 atttatcttt gtgcaacttg ggaagataaa tccttgatag cagataataa agttgagact
     2641 gttaaggcac aaagagaaac tgtagaaaga gaaaaagaaa tggaaagaag aagaatacaa
     2701 gaagaagtag acaaagaaag atatattgaa aaacatggaa tggaaatatc agaaaatgat
     2761 ttaaaagtta agctagaggt tttagtacct caagaatata aggggaagaa atatgaatta
     2821 aaagtaggaa aatttaaaag atatagtatg tattttgatt taactgtaca aaatgaaaaa
     2881 tttagtaatt ctgaataatg taaaaaattt gtaaaagaaa ttgctaatga tcttaaaaaa
     2941 attaaaataa gcaaggctta ttttaatttt cattcaaaag atgacggtgg tatatataat
     3001 tctgtttata ttgattattt tagaaacata caaaataatg tagataatgt tgaaaattta
     3061 gaattcaatg agtttgaact taaaaccgaa gaagaagaga agcgagaaca agagaaaata
     3121 gaacaagaaa aaacagttat aataactaca ttcaaaacag agtggttgac ccactagata
     3181 gaataaagaa actaaaagag ttgctagatt caggagcaat tacacaggaa gaatataata
     3241 aaaagaaaaa agaattatta gaatagataa tatagtaagc acttacaagt atgtaggtgc
     3301 ttttaaattt acaaattatt acattttaat tttatagttt agattttatg atataataaa
     3361 aatatagaag ttttgcagtg tgcgatattt gttacaaagt agggcttaat acttgaaatc
     3421 taagatgttg agggtgcgtg ataagtgtta tcaattgcac tattgcccgc tcactgcaat
     3481 tttaagagta ttgtatatat gtaggtattg gaaatgctaa gtttattttg gggttttaga
     3541 ttaactatat ggaatgtaaa ttagcttgat aataaccacc ctccatagga ttatcagatg
     3601 gttttagatt aactatatgg aatgtaaatg atgacatgat agtattagct aatacagaag
     3661 taaaacgttt tagattaact atatggaatg taaatttttt ttcatcattt aaaacttcaa
     3721 ctatcttata atgttttaga ttaactatat ggaatgtaaa taatattatt gcttttatat
     3781 tttttaaatt tatacttgtt ttagattaac tatatggaat gtaaatttgc attctcccaa
     3841 ccattctaga tattctccat agcgttttag attaactata tggaatgtaa atgcgcaaac
     3901 attaccactt ccaccttttc caaataaacc gttttagatt aactatatgg aatgtaaata
     3961 gccttgtctc aatatgaaag agatttaaga atgccaggtt ttagattaac tatatggaat
     4021 gtaaattgag ttaatagaaa atttaaatac taatgaaaaa gtgttttaga ttaactatat
     4081 ggaatgtaaa taatttttcg agagtctcaa tttacagaag aaattggagt tttagattaa
     4141 ctatatggaa tgtaaatttt taagcgtgtt gcaatatgat tgccttatgt ttggttttag
     4201 attaactata tggaatgtaa attcggttta ttcgtattat atttctataa tcttatttca
     4261 gttttagatt aactatatgg aatgtaaatc ttgatgattg gcgagaatac tatatttgtt
     4321 ggtcagtttt agattaacta tatggaatgt aaattgctgt gatttcacta ataaaacaat
     4381 taataattgt gttttagatt aactatgtgg attcaaaatt aaataaacaa agaaagcact
     4441 tacaaatatg taggtgcttt tattctgctc aaaattggtt ggttgggtaa aataattaga
     4501 aaaagttagt aaaaacctat tgactgtaac tcgttacaat attgttatta atgtaacgag
     4561 ttacagaaaa gaggtgaata aaatagcaac taaaagtagg gcagagtata tgaaaaatcg
     4621 tcgaaaagat aaaagaggtt ttagtgtact tttagacaaa gaaaagttag ataaatttga
     4681 tgaagtatta gaggaaaaga atctaaccaa gaaagaatgg ctagaagaaa aaatcgacga
     4741 ggaactggaa caaaaggaat aaaaaataag ggtcactccc accgaccaaa gtttgagtaa
     4801 cccctatgac gtatactatc gtatatcaat tataatatat gtcattcctt aaaaaaaatc
     4861 aattattaag gagtgtaata ttatgaaaaa tgaattaatg atgtttgaag gaaaagagat
     4921 tgaagtattt gaatttgaag gaagaatttt attcaactca aaagatgtag caaactgttt
     4981 agacataaaa aatgttaatg agaatataag acttatgaat gaaaagcaag ttgtaaagtt
     5041 aagaaattcg gatatcagta atactgacat ccgaaaactt aataatgctg gagaaaattt
     5101 tcttactgaa agtggagtgt ataaattaat ctttaaatca agaaaagaag aagctgagag
     5161 attccaaggc tggataagtg atgaagtttt accaagcata agacaaactg gtgcatacat
     5221 aaataataat gcaataccga aaagttgaga gaaaaagcaa gtgagattga aaaattacag
     5281 ttagtttata acagtacatc tatgttaaaa gaattgctag atgatgcagg ctttgacaat
     5341 aaatcaaaac tattaacagc taaaacatta tataagaaag caggaattga tttaccaata
     5401 gagataaacg aagaagaaca ttattttgat acaaaacaaa tagcatctaa actgaaaata
     5461 tattctaaga gtaataaacc agctcagatg gctgtttgtg agattattaa aaagattgat
     5521 ttagaagatg gtgaagttaa aggtgtttgg gaaactaatg gttcttggac tggtactgta
     5581 aataaatata caaagagtgt aatagataag gtgagaactt ggatagagga aaataataga
     5641 cctaccaaga ttgcagatga gaagaagaat tatcatgtat tttacaaaat tgagtaaatt
     5701 tatcagttgt attaaataat ttagtttagt tttgtgggga ttaatacaat gtgtgagaat
     5761 ttacttgata atatgtatat tgaaaagaga aaagaagaat atagaattaa acttttgaaa
     5821 ataagagaaa cagatataga tatatataat aagttagaaa gtatagtata taaactttct
     5881 gagaaaaaat tagagaaaaa taataaatag acatataaag cacttggata ttctactgtt
     5941 tcaagtgctt tgtttggtat aaaatggtat aatggagata gagaattata ttaacccaaa
     6001 aatggagaaa atagagaaga tgatttagtt gacgctttat cagtaggagt acttgcaggt
     6061 aagacagaat ctctagtacg attagttgga gatggaataa gtgatactca aaaaagttta
     6121 ataaataaaa gagaatttag aaatataact caaattggcg gaaatggaaa tgagaaagca
     6181 tttagtgaag tagaaaactt agttaaatag attagaaggt agcaactaga gttagttgtt
     6241 accttctttt tttatacttt ctctctctaa gtattttctt tttcatatct tctaat
//
LOCUS       DAFKMX010000200         6292 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFKMX010000200 DAFKMX010000000
VERSION     DAFKMX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.203"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            401..1675
                     /locus_tag="LZB08_003262"
     CDS             401..1675
                     /locus_tag="LZB08_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBM7316727.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPCLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYADIPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYVKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1882..2199)
                     /gene="trxA"
                     /locus_tag="LZB08_003263"
     CDS             complement(1882..2199)
                     /gene="trxA"
                     /locus_tag="LZB08_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBM7316728.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2466..3218
                     /locus_tag="LZB08_003264"
     CDS             2466..3218
                     /locus_tag="LZB08_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBM7316729.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3205..4086
                     /locus_tag="LZB08_003265"
     CDS             3205..4086
                     /locus_tag="LZB08_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBM7316730.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4312..5667)
                     /locus_tag="LZB08_003266"
     CDS             complement(4312..5667)
                     /locus_tag="LZB08_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBM7316731.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2408 a          904 c          810 g         2170 t
ORIGIN      
        1 aaaatctttt tttctaatta tatacaatat attttccatt atgttctaat caatttcttt
       61 actattttta caactttatt agtcatattc ataattattt ctaattagtg tttaacattt
      121 tatgtaaaat ttataaaaaa atgtatataa attaaaataa gtgactatac taaaaatatg
      181 atacggataa acaataagtt caacctatct cccccaaaat caagtgagct acagtttccc
      241 ccaactgtag ctcattttcg tgtaatttaa taaacatctc aacaagaatt aattaacgaa
      301 accccttata aaaacataac atatattaaa caagctcttg attaaaactt gagctttttt
      361 aataaaaaac agaatagttt attagataag gggttttttt atgcaattca tggatgtatc
      421 taaatacatt gaaggtgcaa atcttaaagt taaaactaaa aatggtgaaa ctgttaaaag
      481 aatatatttt aacaactctg ccactccatt agttttaaaa aacgtagtgg ataacttaaa
      541 ctgtgagatt ccttgcctta cttatattaa tgctccagga ataatctcag aaaaaaatac
      601 tttaaaatat gaaaatgtac gctctactat cctcaaactt attgatggag acgaagaaaa
      661 ggattctgtt atttatgtca aaaatgctac agaaggaata aacttcttag caaaattttt
      721 taaagatgaa aatccagata aatttgttat taccacagca atggagcata tggctaatta
      781 cctgcctttt aaagttaatt ttcctacaaa agttgttgga attacagaaa aaggagaact
      841 tgatttatgt caattagaga atatacttaa aaattatgct ggtaatgtat ctctcgttac
      901 agttacagga gctagtaatg tcacaggtat aaccactcct atatatgaaa tagcaagaat
      961 agctcataag tatggtgcaa agatattggt ggatattgtg caagtaattc agcataaacc
     1021 tttttcaatg atgccacatg aatgtgatga gcatatagat tttgttgttt ttagtgctca
     1081 taaatgttat tctccactaa atggaggagc tctggtagga cctaaaaaat tctttgaaaa
     1141 atttaatcct gtactatatg gctcaggttc taccaaattt gtaaatgata acaaaatact
     1201 atatgcagac ataccccaac gttttgaagc aggataccca gagtattttg gaactcttgc
     1261 aatgggaaaa gcactgaaca ctttaaataa aattggtcta aacagaattt caaaatacga
     1321 aagagaatta tttttatatg ttaaagaaga aatgagtaaa atacctaata taataatata
     1381 tggagataaa tcaaataatg taattatccc aaatatatct tttaatataa gtaatatgta
     1441 ttacaaagat gtagcaaaat atttagttga taactttgga atagaaactg gagctggagt
     1501 agtaggtgca gatatttatg tacaaaaatt attaggtata agtccaaaag aagcatatgt
     1561 cagatttatg agggaaaatc ctgtaggtct tgttaggata agcttaggaa tgtacaatac
     1621 ttttgatgag gtggatagct ttatacatgc tattaaaact ttggctttta aataagtttt
     1681 ttctattctt ttttatcaat aaatagagag tagttttgtt ttcacaaaaa agcaaattat
     1741 atcaacagtc aatttcatta gcaattagac actcatttgt aactatacaa actattttac
     1801 actatacaaa caaaaaactc attacaatga gtttttttgt ttatatatga gatttatttt
     1861 aattacttca atttacttta attacatata tgattcaatt ttgtttttta aattctcctt
     1921 aggcataaaa cctattaatc tatctacagg ttttccatcc ttaaatatta gcatagttgg
     1981 tactgtagat acttcaaatt tctgagctaa ttctatactt tggtctatgt ccaccttcaa
     2041 gaagtttgct ttttcaacca tctcatttcc taaagcttca tatattggtg atagcatctt
     2101 acaaggtcca caccaagtcg caaagaaatc tacaactact aaaccatctt tattttctac
     2161 ttcatttata aattcattat tatttattat ttttaccata atatacatct cctttattat
     2221 cttcttgata ttaatatact acgccctata taacagttca attaggcact tttttgtaac
     2281 cgcctatttt taataggttt aatattatga taaatagatt tataagatta aatatttatg
     2341 agactaaata tttctaatat attttatttt tttcttaata tagttgacat tttttccctt
     2401 aaattataaa atcaaattaa taactacata ggtagttact aatcttttat caaaggagtt
     2461 tctatatgga taatataatt aatgaattac aaaatatagg atttactaaa tacgaatctc
     2521 aaatatacat atcattatta aaggaaagcc ctttaacagg atatgaaata agtaaattat
     2581 caggtgtacc acaatcaaaa gtctatgaaa atataacaaa gctattaaac aataatataa
     2641 ttatcaatgt tggaactgac cctattaagt acataccaat aaaccctaat gaattattaa
     2701 aaaataagaa aaaagaattt gacaattcta tggataatct aaagaatagc ttacagatat
     2761 taaataaaag taaaagtgta gaatacgtat taaacataaa aggagataaa aatatattaa
     2821 aaaaagcaat agaaatgata aaaaatgcga aacaagaaat tataatttct tgcccttatg
     2881 aagaaattct tgaattaaaa aaagaactcc taattgctta caaaaaaaat attaatattg
     2941 aaatactact tttagaaaac aaaagtctga aaggtcttga taatattcat attcatggag
     3001 gtaataaaac tacacttgaa aataaaatgc tttacaaagg aattattttg attgtagata
     3061 atgatgaaat attgacagga aacttatcag aaggcagtga ggccatttca atttggacaa
     3121 aaaactctaa cattttatat attggagttc aatacataaa acatgaaatt tatatttcag
     3181 aacaaataaa aggaggttat aaaaatgaac aaaattaaga ttaaacaagt tgatgctttt
     3241 acaactatac cttttggtgg aaatcctgct ggtgtcgtta ctgatgcctc taacatttct
     3301 gatgaaatta agcagaatgt ggctaaagaa atgaatttat cagaaactgc atttgtttca
     3361 gagtcaaata ttgcagattt taaagtacaa tttttcacac caggtttaga agttgattta
     3421 tgtggtcatg caacaatagg tactttccat gcacttttgg aagaagggaa gcttaatact
     3481 aataagagta tattttatca agaaacaaaa gctggtgtat tatctgtaga gcttaagaaa
     3541 ataaataatg aaaatatatt tatgatggaa cagaatacac ctgaatttga aaatttagat
     3601 aattgcagaa aagaacttgc acaaatgata ggtgtgcatg aagatagact tcttgaatat
     3661 cctattataa aggtaaatac tggactttgg tggcttgtat ttggtctaaa atctttagat
     3721 gatttaaaaa atatctctcc agatttaaat aaaatcaaaa aatttagtgg tgataataat
     3781 ataattggta taactccatt ttgtatagaa actatagata aaaagtgtga ctaccattta
     3841 agatctatag caccatatgt tggagttaca gaagaccctg tatgtggaac tgggaacgct
     3901 tgcgtatcat cttatatagt acatcacaat attttagata aaactagttt tattggtgag
     3961 caaggtaact ttataaacag acctggaaag gtatatgtaa atataactag agaaaacaat
     4021 gacataactt ctgtaaaaat tggaggaagt gcttatactg ttttaagtgg tgaaattatg
     4081 tattaatccc atacctaata aaaacaaata aaaatactag ctaactaaag ctagtatttt
     4141 tatttgttat ttttatttaa taatcatact ctaacaaata ttataattat atatatcata
     4201 atattgtatt tataagtaaa attttatttt tattattaat tagttataaa tcaatctaat
     4261 acaacataat tacagtagat taacctataa aacataataa ttgcttacta attatttatc
     4321 atcctcaaca agtctactta atgtcttatc agataagaat aataatatcg ctgcaacaaa
     4381 tagtattact ggaattatag tatatacttt aatcatatca aatttaagag taaatgcata
     4441 tatatatcca tatcctttac cagctataaa agtagcaaat gtccatactc ccataagaac
     4501 acctagaagc ttcttaggtg catacttact tacaaaagag ttaccaagtg gtgagaataa
     4561 catttcacca agacttaaaa gaataccaaa catgattatc catagtatac tagctttagc
     4621 agtttcagga gctccaactc ctcttgaaaa ttctgcacca acaagcatta agaatgcaag
     4681 acctaagaat attaatccaa gacctgtctt tttaaataaa ctcatatctc cttgtggtct
     4741 actagcaagt ttaaaccata atgctccaag aactggtcct aatactatac atacaagtga
     4801 atttaatgag tcaaaccaag caagaggaac atcaaatcct ccaacattca tgtttacaaa
     4861 tgctgcacca taatcataaa ctgcaagata agttagatac cagaataccc agaaaattac
     4921 agagaagaat gatataagta taatagccca aattctcttc ttatccatgc ttgtaagagg
     4981 agacttatct ttttcttcaa ttttttcagc aactacacct tctttaaaag gtctctttcc
     5041 tgcattccca aggaatctcc atccatatat gaaccatatt gcacctatta cacaaagtac
     5101 agctgctata aagaaacatt gagaaaatcc aagtacacca ttttttgcaa atgttttaag
     5161 gtaaagaata ccaactgcta tagtaccaat aaaagaacct atattaacaa aagaatactg
     5221 tactgaaaat gctgtatcaa gttcttcttg actatcaaaa agctgtccat ttaccgcaga
     5281 tacgttacct ttaaagaatg cagtaccaat tgaaactaag attatcattg catttaccat
     5341 agaaactgta gttgcaatag aaccaagata gtatcctact cccatgataa gcatacctat
     5401 aggtatagta tatctagctc ctatccatct atctgatata tatcccccaa ttataggtgc
     5461 aaggtatgtg aatgcaacaa ggttggcttg cattatagca gcttgtcctt tatctattcc
     5521 aagaccacca tgaactatag ctgttgtaag gaatatatat attaagtact ttgaagcata
     5581 atatgctgct ctttcaaatg agaaagttgt tgagcagata taaaatccca taggaaattt
     5641 acccttcttt tttactgttg agctcataat tttaaatcct ttcttaattg tatttttatt
     5701 ttttacgttt tgtccaaatt ctttctttta tgaaagatat aatattatta ttttgtaaaa
     5761 caatttgtaa ttattattaa gataattgcg catttatttg tagagtacat attctcatgg
     5821 aataaaatta atacacttct taatcataaa tttaaattat ataaattcac tattttcaga
     5881 tatagttata aattaatata ttctcatcta ataccatgaa aaaaccttcg ccatatgcaa
     5941 ttcttatttt ttacgtgata gtattttaca aactaaaatc ccccttttgt agcaacttat
     6001 gtaattacat tttagtgctt ttttaaagga gtctcgaata aagcaacata tattttgttc
     6061 aaataatgtt tttatttatt ttttaaattt atatattact tcacccaacc aattctcaac
     6121 ttaaataact aaactactat ctattcacag attaggctta tttcttacac attagtttat
     6181 aataaacact gtgaatctaa acataaaaac tgttatttta aagtaattac ccatatattc
     6241 gctttgatac tatttattga ggttatactc tttatgtata atatattatt ta
//
LOCUS       DAFKMX010000201         6242 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFKMX010000201 DAFKMX010000000
VERSION     DAFKMX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.204"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(248..655)
                     /locus_tag="LZB08_003267"
     CDS             complement(248..655)
                     /locus_tag="LZB08_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316732.1"
                     /translation="MSKVAKAAFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1129..2013)
                     /locus_tag="LZB08_003268"
     CDS             complement(1129..2013)
                     /locus_tag="LZB08_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7316733.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2304..2780)
                     /locus_tag="LZB08_003269"
     CDS             complement(2304..2780)
                     /locus_tag="LZB08_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBM7316734.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3047..4222
                     /locus_tag="LZB08_003270"
     CDS             3047..4222
                     /locus_tag="LZB08_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBM7316735.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4303..5106)
                     /gene="proC"
                     /locus_tag="LZB08_003271"
     CDS             complement(4303..5106)
                     /gene="proC"
                     /locus_tag="LZB08_003271"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBM7316736.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5146..5730)
                     /locus_tag="LZB08_003272"
     CDS             complement(5146..5730)
                     /locus_tag="LZB08_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316737.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2062 a         1057 c          698 g         2425 t
ORIGIN      
        1 ttacctgctg attgcccatt ttaaagtact taggatttaa ccttatacca tctaaccaat
       61 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttgat
      121 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      181 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacgagtg
      241 tgtgtagcta ttttttaatc aatattttag taaattttac ggtaagtaaa aacctctctc
      301 cttcaaatcc tatagcaaat atacttacaa gttgctttga gaaaataact cctaatatag
      361 ctacaactat gcatatacca actattatat ttaaaacatt gtttaaaaac tttagtgctt
      421 gcttttcacc ttgtttacta cttatatctt gatacattgg aataaaagta gtaactatag
      481 cagtacctat tgctgcaaat ataatatttg gtatattcat agctgttaaa taactctctg
      541 tatataagcc tgtaccataa attgatgata atactaattc tctacccatt ccaagaatct
      601 tagagattat agtaactatc atcaaataaa aagctgcttt agctaccttg ctcattttat
      661 cactccactt ctaacatacc tctttttaat caagtaaaat ttagctcatt agatataatt
      721 ctatcaacta atttgccttg cttagcccta tcaaaatata attaacacct atttcttaat
      781 atatcacaaa ctacattatt cttcatattt ttttatttct ccaataagtt ttacattttt
      841 cctatataaa ttgtatagct tcaattatat tcaaagaaat ggagttgcta cccgataact
      901 ttaaaagtaa aaaacagatt ttattatctt tttaaactat attctaacat attttgtcca
      961 tattgagaaa ttttactaaa ctattatgta ctttattaaa taaataaaat aacagcacac
     1021 ctataaaata caagtgtgct gttcataaac agattttata aaaaaataat tcctaagtag
     1081 agattaacat gctttctcat actctcatat ataatgtgta taaaaatatt aataaaaatg
     1141 ctttaattgt tttaacttac ttggatgtct taattttctt attgctttag cttctatctg
     1201 tcttattcgt tctctggtaa ctccaaatac ctttccaatc tgttctagtg ttttagggtc
     1261 atcatcatca attccaaatc tcattcttaa aacttgctgt tcttgctctc caagacctgt
     1321 aagtagttct tctatctttt cttttaaatt atgctgttca acttcgtgta ttacaacatc
     1381 tttaaaatca gcatctgatg gtataaattc aactaaacta ctatcctcat cttcttttaa
     1441 tggagtatcc aaagatacaa catttttatc tcttcttaat aattccaaca ctttgtcaac
     1501 ttctaagcca catgcatctg caatctcatc tatagttggc tctcttaata atacatttaa
     1561 aagttcttgt ttctttttct ttacaaaatt tattctctga tgaagatgta taggtatcct
     1621 aatagtattt tcgcaatcgt cgatatatct tgttatactt tgttttatcc accaagttgc
     1681 ataagtgcta aatttgtatc cttttttata atcgtacttt tctacagctt ttataagtcc
     1741 tatatttcct gcttgaatta aatccaacat atcaatacta tctcttttat atctctttgc
     1801 tatgctcact accaacctat aatttgaatt tataaatttt tcttttgcta ctgaagatga
     1861 gttgattatt tccttagcca gctctacctc ttctcctgct gacaacattt tgtattcctc
     1921 tatttccttt aaatacattt tcatcgcatt tgatacattt gatgaaccca cacaaatata
     1981 attttcataa ttattttctt ttttagtatc cataatcaaa ccccttctat tcatagaatt
     2041 tgctaaaaca aaaataaata atttaaacct acataatcta caattacata atatcccatt
     2101 ctttaaatga attgttttgt atattatgta tacaaattta taaaaactat acctataata
     2161 ttattctact tctaacagca atatcctcct tatatatata atttatttta ttatatgttg
     2221 ttatattctt agttttttga atgatatatg tctaaaaaaa gactgtttac taaacagtct
     2281 ttttttatat atatgctcaa tatttaattt tcatgatgat gattacatcc acatccacaa
     2341 ttttcatcat ggtgatgatg ttcttctaaa tcttttacct gttgttccat ttcatgaaac
     2401 tcttttacca ttatatcata agttggaaga gctgcctcag tatcgtattc ttcccctctc
     2461 caatcagcgt acatcatagc atctccatta gttaaaacca atctacctga catagttata
     2521 ttatccactt cttctaaaaa ttctatttgt ttttgtaaaa cttctccttc taactccaca
     2581 tcttcatcta aacattgcac tatcattaca ggtgcataat aatcttcttc atctttaaca
     2641 tttacaacaa tatctaaaat tccttctgca ccttcttcaa attctgccaa ctcaacattt
     2701 gtatcaatca tatcttgagg tactaataaa tcttctatta aatattttat tactctatct
     2761 tttactaatt cttttgacat ttatttttct ccttccgatt ctataacatt atcataatca
     2821 tttctatatt atagtatact acattaattg ttttttcaat ctcttacaaa aattattttt
     2881 tcattatctt ccttatattc ccttttttat tacaaagttg taatcgcatt gatttaaaaa
     2941 atagtttata tttagtataa tagaaatgta gcacatattt ttattcaata tattaataaa
     3001 aaaatagatt gaaaatgtaa tgtatgttaa atctaggagg aatattatga agtttaaaaa
     3061 acaaattagt tctattgttg tgtctactat gcttgtactt gggagtatga acctttctta
     3121 tgcagacggt acaaatgtag ttacacttgg agctaactta tcagagtctc aaaagcagca
     3181 aatgcttaat tactttggag taaaaaaaga ccaagtctta gttttagatg taacaaatgc
     3241 agaagaaaga cagtatttac aaggtgttgc aacagagggt caacttggaa aagttactat
     3301 ctcttgctca tacgttgaac ctactaaaaa aggaaatgga attaatgtta aaacagcaaa
     3361 cttaacttgg gtaactagtt caatgattgc ttcaaccctt acaacagctg gtcttgaaga
     3421 tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt actggagcat tgacaggaat
     3481 catgaaagca tttgaggatg cttcaggaaa gaaacttgat gaaactaaga aagaaattgc
     3541 ttctgaagaa ttggtagtta caggtgactt aggagatgat atagggcaag aaaaagcaac
     3601 aggtgtaatg aatgatataa aaacagaaat tgtaaagaat ggtacaaaag ataccaatca
     3661 aatagcaaat acaataaata atgttgtaaa taattacaat attactatta caccagaaca
     3721 aaaagaacaa attaaaggtg taatgaaaaa aatagctgac caaaattacg attacaatag
     3781 catgaaaaat acactagata atgtatcaga taatgtaaat gaacaattaa agaaattagg
     3841 tgaaagtgtt aaaggttctg gtatactaga tactattggt ggatggttta gtggaatagg
     3901 agactggttc tctggtttat tctcaggcgg agataaaaaa gatttgggaa tcttaaataa
     3961 cactaatgat gagtctcttg gctctgatgc tgtaataaat tctacagaag gtgttcaaat
     4021 aaatccacaa aataatacta attctaacaa tgatagtgat acaaatagct caaaaaatga
     4081 tacttcaagt aataatgaaa ataacgatag ttctcaaaca aatgaaaaca acacaacaaa
     4141 taattcagaa aatcaatcaa ataataatga ctcagttggt ttctttgaaa aaataaaaaa
     4201 ttggctttca ggcttatttt agaaattaaa caaataaaaa gaattgccta aaatggaaat
     4261 tttctataaa aggcaattct ctttatttac aataaatttt tattactcac tcattttttt
     4321 agatttttct atacatgcac atattgcttt tatcacagaa gctctaaaac cttcttcttc
     4381 aagtacttta actgcttcta tagtagttcc accaggtgaa cataccatat cttttaattc
     4441 accaggatgt tttcctgtct caagtaccat ttttgcagaa cccataacag cctgtgatgc
     4501 aaacttatat gcttgtggtc ttggcattcc tgcaattact gctgcatctg ctatagcttc
     4561 tataaataaa aatacatatg ctggtgcaga accacttgca ccaattacag cttctataag
     4621 atactctggt actacctcag tatttccaaa agaattaaat acttctgtaa ctgcttctaa
     4681 gtcttctttg tttatatttt tatttataga aagagaagac atggcttcat ttacaagagc
     4741 tggagtattt ggcatagttc taactatttt tttatcttct cctataatag actctatatc
     4801 ttttatagat tttcctgctg ctattgtaac tataatttta ttgtcatcta taaagtcttt
     4861 tatctcttcc aaaatatcat catatatatc tggtttaaca gctacaatta ctatatcaga
     4921 ggttctagtt acttccttcg agtcttttgt agtatttatt ccaaaatttg catctattct
     4981 atcaagagtt ccttgactat atgctgatgc tataaccata ttaggttcaa ctaattttga
     5041 atttactatt ccccctatca tagcgcttgc catattccct gcacctatga atccaatttt
     5101 tttcacttta gtacctcctt attgactaat agtaatcgtt aaaatttaag cttctttaac
     5161 tgcatcagac ttttcaaatg gatttggtat acttactacc aaaattttca ctaccttatt
     5221 agtatagttg acccaattat gattgttctt ttcactatta tagtgtattg tgtctcctgg
     5281 atacatttct atttgttcat ctcccaaaaa cactgtaaga gtaccctcta atacatacac
     5341 aaactcctca ccttcatgta cgtaacaaca aatttcctca ctactcttac ttggaagaat
     5401 ttcaattaat cttggtagca ttgttttttc ctttaaacta gatgataaat gataatgtat
     5461 aaatgttgag ttttctacat caaatacttc tttttcataa cttcttaata cagctctttt
     5521 atgctctttt ggcttcataa aaaagtaagt taattctaca tctagtacac ttgctatttt
     5581 tcctaaggaa tctgttgcca cacttgttaa tcctctttca agttgtgata aaaaaccaat
     5641 tgacaagttt gttttttcac tcatatcctt cagcgtcatt tgcttttgag ttctgagttg
     5701 ctttattttt gctcctatgt ccttattcaa aaccatcacc cctaaataat tttaattttt
     5761 tcataatata aatacaaaaa tataaatata ggtactgaac ctatctttat ttcatttaaa
     5821 aaaattttta taagtattgc tataatttat caatcttttt ttgtctacat ttttacgatt
     5881 atttacgatt tatttttcac atatgtatat ttaatcttgc caactaaaga tacatttttt
     5941 ataagcattt actctttata taataaaact taagttgttc atgagtcttc ttctggttta
     6001 attattaaaa tttcaaatta tattattatt tctacacatt attacatcct ttagtacaat
     6061 tataacataa ttttacaatt taatgatatc tattcaatgt ctttttttac tctacaacat
     6121 attttatttt agagtttaat actatatata ttttttatat acagccttgt cattttttag
     6181 attgtcaaga gttattgaag tattttatgt agatttagat tttattttct aatttctcat
     6241 tt
//
LOCUS       DAFKMX010000202         6087 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFKMX010000202 DAFKMX010000000
VERSION     DAFKMX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.205"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            325..846
                     /locus_tag="LZB08_003273"
     CDS             325..846
                     /locus_tag="LZB08_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316738.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1213..2388
                     /locus_tag="LZB08_003274"
     CDS             1213..2388
                     /locus_tag="LZB08_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316739.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2422..3960
                     /locus_tag="LZB08_003275"
     CDS             2422..3960
                     /locus_tag="LZB08_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBM7316740.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3992..5194
                     /locus_tag="LZB08_003276"
     CDS             3992..5194
                     /locus_tag="LZB08_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7316741.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5361..5960)
                     /locus_tag="LZB08_003277"
     CDS             complement(5361..5960)
                     /locus_tag="LZB08_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7316742.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
BASE COUNT         2292 a          746 c         1072 g         1977 t
ORIGIN      
        1 atgctctaca tttggataac caatactatt ttactgataa tcttttcctt atcactaatc
       61 ttacatttta aaatattatt tttactcgta atattatcat aagaatttgt ttcttattta
      121 aaactaatta gaattataaa gtttattgcc atgttattaa taaaataaat ccagttaaat
      181 cgagtgctag ttaaagtaat aagaaacttt tacaatggtt tcctttatcg attgaaataa
      241 aagttaatat ggtataatat ctaaagagag aatcttatca attagaatat gcatagaaac
      301 aagtggctta aaaggagacg ttatatggaa gagatagtaa agaaggaaca agcaagtatg
      361 aatgatattt ttttgtatct taaagctaaa tcaaactcat tatataaatt tgtaatatta
      421 tataatgagt ttatcaataa acctaaagat tatggaactg gggaacttat aaacatggtt
      481 gaagtacata tgctaacagc gattgaagaa aatccaggaa taagtccaac tcagttagct
      541 aaaatatgga acagaactaa aggtgcaata tctcaaacta tatctaaatt ggagaaaaag
      601 ggttatataa ctaaagaaaa gaaagaagga aatgcaaaaa ctatattact atatccaacc
      661 acatcaggag tagagttaag taaggcacat aaactatatg acaccataga tgttacaaga
      721 acatttgaat acttaatcaa ggagtgtaca ttagaggaga tagatagttt ttataaagta
      781 attgatttgt ttattaattt attagaagaa tctccgcagg aaaataatga ttctgaaaaa
      841 gtgtagctga gattgaagtt gaataaagag aaatagatat tatagaagta gtacttaagt
      901 acacattata taaaaatata ataggtatag gattaaaaag tttagtggtt taaagtttaa
      961 ccactaaact tttttaatgt ctataatcta ctattattta atttactaaa aataaaaaaa
     1021 ctcattatat taagataaaa atagaaaaat actataatat atcagtttaa tacagaaaaa
     1081 agttaaaaaa tttgagaaaa ataaaaatga aaacgtttta tattgacata gagaaaaagt
     1141 agtgatacca tttaaatata gtttagtagc taaactatat ttaaattata ataactattt
     1201 tagggggatg ttatgaattt aaaacaatta agtgaaaaat acgaagaata tgtaattcaa
     1261 atgagaagag aatttcataa gtatccagaa ttatctttta aagaaataag aacaacccaa
     1321 agaataaggg aagaattaca aaaaataggg gtcccttttg tatctattga accaaatatc
     1381 gtaatagcta ctatacaagg caaaaatgaa ggtaagacaa ttgcaattag aggagatata
     1441 gatgcgcttc caatgcaaga agattcagat gttgaataca aatcagaata tgatggaata
     1501 atgcattctt gtggacatga tgctcatgga gctatgttac taggacttgc acatatgcta
     1561 aatgatataa gaaaagattt aaaaggtaga gtattattag tttttcaagc agctgaagaa
     1621 gttggtggtg gacacaagga gataatacag tactttaagg aaaatggagg accagataga
     1681 ttaatagcta ctcatgtttg gtccgatatt gaagctggta aaatttctgt tgaaccagga
     1741 cctcgtatgg ctggtgggtc tggttggaga atagacataa caggtagagg aggtcatggt
     1801 tctagaccag atcaaagcat agatcctata aagccagctt gtgatatagt tttaaaagta
     1861 tcttctatac cagttaatca tataagtgta ctagaacaat gtgttgtaca tgcatgtgca
     1921 atacatggag gaactacaat aggaaatgtt attccaaaca atgcagaggt aataggtgga
     1981 tatagatatt ttactgaaga atcaggcaaa aaagtgtttg acattataaa gcaaatgagt
     2041 aatggtgttg gacaagttta tggagtggat gtgaaggtta ctcatactgg aggcatagga
     2101 cctgttatca atgatgaaga agcagttaag atggctaaag aaatagtttc taatatagat
     2161 ggattggagt tagattctta tgagccaata tgtgcatcag attgttatgg tgaaatatta
     2221 aaagagtatt ctggctttta ttgttatcta ggggtaggta acaaagaaaa aggaataata
     2281 tatgcacaac accatcctaa gtacaatata gatgaagata cattaaagtt aggttgcgaa
     2341 tttatggcta aatatgcagt tgaattttta aataaaaata ataactaaaa taaatcgaat
     2401 tttataaatg gggggatgaa aatgcaaggt gcattaaagg aaaataagaa aaaaagtggt
     2461 ggaggatttt taggatggat tgaaaaagtt gggaataaaa tgccacatcc tctagcatta
     2521 tttacatata ttacattgat agtagtagca ttatctgcaa tagcaagctt tttaggtttt
     2581 tcagcaacaa gtcctgctac aggaaagtta ataaaagtta tgaacttaat aagtgcaaaa
     2641 ggtttagtac tatttatgca agagtttgtt aagaacttcc aaaacttccc tgtgcttgga
     2701 gtagttttag ttatggcagt tgccacagga gtttgtgaaa agacaggatt tttttcaact
     2761 gccataaaaa tgagtttatc aaaagttaag gggaatgcgg ttgtatttat aatagctttt
     2821 ataggagtat ttgctaatca agctggtgat gctgcttttg tattagtacc aactcttgca
     2881 ggagcaatat tttatggatt aaatagaaat ccattagctg gtatattctg tggatttgca
     2941 tcagttggtg gtggatttgc aacagctgta attcctgggg gatgggacgt tacgctaacg
     3001 ccaataactg tatcagctgc tcaaactatt caacctgcat ttgatatgac attattagct
     3061 agttattatg cactatttgt atctgcgttt atagtagcta cagtatcaac aatagtaacg
     3121 gttaaattta tagaacctaa attaggtaag tatactggta aaccagaagg tatagatgat
     3181 aatcaaggtt ttgaggtcac aaaagaacaa gcaaaagcag caaagttagc aggttttaca
     3241 gttttagctt atgtagcgtt attaattgct ttgtgtatac cagctaatag ttttttaaga
     3301 agtccagaag gttctttaat atttggagca ccacttatgt cttgcttaca gttttttata
     3361 gtaatactat tcttcttgcc agggattgtg tatggaatga gggttggtaa aatcaagact
     3421 gtaaaagact taaatgatat attatgtcaa tcaatggcag caatggcacc gtttatcgta
     3481 cttgcaatag ttataggtca attcttaacg ctattctcag tgtctaattt agcacaggta
     3541 ctagctataa aaggtggagc attactcaac tcattgccac taccaccaca agctataata
     3601 ctgctattct tagtactagt agcgtttata aatatatttg ttataagcgg aagtacaaaa
     3661 tggatgatat ttggaccagt atttgttcca atgttaatgc aattaaacat acatccagca
     3721 tttactcagt ttgtatatag attaggagat tctgtaacca atcatttatc accattaaat
     3781 gcattcttta taatactttt agcaacagtc caaaaatacg ataaaaatgc aggtatggga
     3841 actatattct cagctatgat accttacact ataggttact ttgtagtact tgcagtttta
     3901 gtagtagttt ggatgacaat aggtatacca gtaggaatag gtggacaagt ttggttataa
     3961 aacatattta ataattttgg gaggaaagaa aatggaaaca actgaaataa gatacaattt
     4021 tgatgaagta atagatagaa gtggaacaaa ctgtcttaaa tatgatctta agagtcaatt
     4081 tatgccagaa tctccagaag atgcactacc actatgggta gcagatatgg attttgcatg
     4141 tgcccctgag ataattaatg caatgcatga aagaatagac agaaaaatat ttggatatag
     4201 ctctcaacaa actgatgatt attatgaagc tgtatgtgga tggtttaaga gaagatttga
     4261 ttggaaaata gaaaaagaga atatattctt tagcccaggg gttgtgcctg cgttaggatt
     4321 tttcacacaa atattaacac aaaagggtga tggtatcata atagaaaagc ctgtatacta
     4381 tccttttgaa gataaaataa ctaataatga aagaaaagta gttaatagtc ctttaaagtt
     4441 tgaagatggt cattattcta tggattatga tggatttgaa gaattagcta aagatgaaaa
     4501 taataaggtg tttatattat gtagtcctca taatccagtt ggtagagtat ggaaaaaaga
     4561 agagcttaga aaaatagtag acatatgtaa gaaatatgat ttgtggataa tatctgatga
     4621 aatacattgt gatattattc gtaaaggtga aaagcatata ccattagaag tagcatgtcc
     4681 tgattataaa gatagaataa taacttgtac agctccaagt aagagtttta acttagctgg
     4741 tatgcaatta tcaaatatag taatcaataa taaggaatta caaaaaaaat ggatgcttga
     4801 aacagattct aaattagcta tattcctacc taatccattc gcaatagttg ctactaaagt
     4861 tgcttataat gattgtgaag attggatgaa tcaagtaaat gagtatatag atggtaatat
     4921 taaatatgta gaagattttg ttaatgagta tatgcctaaa gtaaagataa taaaatctca
     4981 aggaacttac ttagtttggt tagactttag agggtatgga ttagatgcaa aacagcttga
     5041 atatgtaatg cttaacaagg cgaaggtatt gctagatgaa ggatacatat tcggagaaga
     5101 aggagcagga tttgaaagaa taaatgttgc ttcacctaga acaattatag aagagtgtat
     5161 gaatagaata aaatgtgcat ttgaaggatt ataatagtaa tatctaaata atatatttgt
     5221 gtattttaaa aaaagactat gctaaaaaaa tatttcaaag catagtcttt taaagtttta
     5281 tgatgagttt aaaaaatttc aaaacgttac tattcactta tatgtttata ttagaaataa
     5341 ttccacaata tactcatttt ctaaagttgc acttttatac accttgcttc accaatatca
     5401 tgtgtaacca aaatagtaga cttattctca ttcttaataa tactataaac atcatcacaa
     5461 actaataatc tagtctgata atcaagagct gaaaaaggtt catctaaaag tagaatatct
     5521 ggatttacag aaagtgttct aataagcgca actctttgtc tcatacctcc agataattct
     5581 tttggataca tacttctaaa atcccataat ccataagtct ttaaaaggct ttcaactcta
     5641 tctaaagatt ttttatcttt tttgccttga atttccaatc caatagttat attatccatt
     5701 atatttctcc attcaagcaa attatctttt tgaaacatat atccaatatt tccagtaatc
     5761 tttatatcac cactagtcgg ttttaaaaga ttagtaagaa tatttaatat tgtagattta
     5821 ccacttccag aaggaccaag caaagttaat atttctcctt ctttcaagtt aaaattgaca
     5881 tcctttaata cttcaagctc gccttcctta gtgtagaagt tcatatttat atttttaact
     5941 tctactatgt tgtcattcat tatgccaccc cctataactt ttagctcgta tatattatgt
     6001 tattaacaat atgacctatt tgtaccattt ttcataaata taatataaat attttgttta
     6061 tttacgtatt ttcttagtaa cttgtac
//
LOCUS       DAFKMX010000203         6024 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFKMX010000203 DAFKMX010000000
VERSION     DAFKMX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.206"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            131..943
                     /gene="modA"
                     /locus_tag="LZB08_003278"
     CDS             131..943
                     /gene="modA"
                     /locus_tag="LZB08_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7316743.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            953..1744
                     /locus_tag="LZB08_003279"
     CDS             953..1744
                     /locus_tag="LZB08_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316744.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1746..2441
                     /locus_tag="LZB08_003280"
     CDS             1746..2441
                     /locus_tag="LZB08_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7316745.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2428..3159
                     /locus_tag="LZB08_003281"
     CDS             2428..3159
                     /locus_tag="LZB08_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316746.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3473..4273
                     /locus_tag="LZB08_003282"
     CDS             3473..4273
                     /locus_tag="LZB08_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBM7316747.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISIFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4813..5529
                     /locus_tag="LZB08_003283"
     CDS             4813..5529
                     /locus_tag="LZB08_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316748.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2347 a          728 c          847 g         2102 t
ORIGIN      
        1 gattttttct taactataca taacaaaaca taacaaaaca ttataaaaca taacaaaaac
       61 ttagacatcg ttttcttagt atgatatact aaaaaagaat ttacactaat ttaataaggg
      121 ggaatatctt atgtttaaaa aactttcagt actagcttta gctatgatta tttcattaag
      181 tattactgca tgtaattcta aagaaaacaa agctaatgat gaatctaata ctaaagtgac
      241 aacagctgat gattcaaaag aaaaagagac tctttttgtc tattcagggg caggtttaaa
      301 gaaaccaatg gatgaaatag caaaaaaatt tgagaaagaa aataatgtca aagttgaata
      361 tagctatgca ggttctgcac agctaatagc tcaaattgag acaagtcaca aaggagacgt
      421 atttattgtt ggttctgaac caatttattc aaaggccttt gaaaaagaac ttgtaggtga
      481 acataaaacg gttgcacatc atacacctgc aatagttgta cctaaaggaa atccagctaa
      541 tattcaaaaa ttggaagact taaataaaga tggagttaaa cttattttgg gagacaaaga
      601 gtctaatgct ataggtaaga ctacacaaaa aatacttgaa aaaaataatt taaaatctat
      661 aaatgataat gttgtctcta cagctgcaac tgtaaatgaa atgattgtac agttaacatc
      721 aagtaaagct gatgctacaa tagccacaaa agattcggtc tttggtaatg aagatgtaga
      781 agttatagaa attgcccaag ataaaaatat agaccaactc atatcagcag gaatcgtaaa
      841 ctatagtgat aaaaaagatt tagctaataa atttataaat tatatagagt cagacgaagc
      901 taaacagata tttaaaaaat atggttttga acctgttaag tagtgattaa acttgaaaaa
      961 taaatttttt actactctac ttactggatt ttttgctata gtctttttgt ttattgtatc
     1021 accacttctt atgttaatca ttaaagggat aagttatgtt cctatatgct taaaatcagt
     1081 ggaagtacag tatgcaataa cacttagtat taaaacatct ttaatatcta ctatagtatg
     1141 ccttttatta gctattccag tagcatattt tttacatatt actaaattac catttaaaaa
     1201 actgattata cagataataa atctccctat gtctcttcca cacttagtat caggaattgc
     1261 tcttttactt ttgtttggaa gaatggggat tggagattcc atttataaga tttttaagct
     1321 tgattttata tttacaaagc aaggtatagt tttagcacaa atatttgtaa atcttccact
     1381 tactattaaa atacttcaca cttcattaaa tgaaagtaat gaaaagatga tatttgttgc
     1441 acgtaccctt ggatgtaata gttgggaagc atttagattt ataattcttc caaatctaaa
     1501 aactggaatc atatctgcaa ctgtaatgac ttggtcaaga gcacttggtg aatttggtgc
     1561 agtagctatg atagcaggtt ctacacgtat gaaaactgaa attattccta catctatata
     1621 tttaaatatg tctactggtg atatagacat tgctatcgga atagctgtaa ttttgatatt
     1681 tctctcactt acatgcttga tgctttttga gatatttttt aatagagagg ttgataagaa
     1741 ttaatatgtt aaaaataaaa aatttatctc ttgatttacc taattttaaa ttacaagaaa
     1801 atagtttaaa tattaataat catgaatact ttgttttatt aggagctagt gggtcaggta
     1861 aaactctatt tctagaaaca ttagcaggaa gatatccatc tgctaaagga aatgtatcat
     1921 acaaagaaaa tttaatatca aacttacctc cagaaaatag aaatatagga tttgtatatc
     1981 aaaattttga attatttcca aatatgaatg ttgaagataa tataaggttt ccacttaaat
     2041 tgagaaaaat acctatagat gaacagattc ttaaggctaa ccacttgtct aaattaatgc
     2101 agatagaaga tataaaaaat aggtatccta aaaatttaag tggaggcgaa aagcaacgag
     2161 ttgcttttgc acgtgcctta atagctgaac ctgatatatt acttttagat gaacctatga
     2221 gttcattgga ttatattaca aaaaaacata taagcaaaat tttgaaggat gtctacataa
     2281 aatacaaacc aacagtgatt catgttactc atgatatttc tgaagcaatg ttttttgctc
     2341 ataagatagg aataatgaac aatggaaaaa taaatcatat ttttgattta aatgaagaaa
     2401 taaaaaataa aggagaatct tttttctatg aatatatttg atattttgag agattatcta
     2461 ttagtacaag tcgataaata taatttaaat atggatatga taagtattgt gtccaaatct
     2521 ctatcttcaa aagaagcaat tggaaatact aagcgtaaag atttcccaat tatagttggt
     2581 aaagaaataa tgcttgaagc tgactttaaa ggagctaaag gacaagcatt tacttctact
     2641 ccatctacat ttgaaggttc tttaaaagac atattatcat tagatttaca tgacaattct
     2701 catgatagaa gtctttttat agcatcatta aatgcagtta tgaaatactt aggcaagacg
     2761 gatagaacca ttcattgtaa aaataacgag ccagaggtat gtgcaaaaaa attccctgag
     2821 tttataaaaa tggaatttgg taatccaaaa gtagcaatta taggatatca accagccata
     2881 attgataata ttaaggactt tttcgaaaca agagtattag atttaaatcc tgaatttgtg
     2941 gatactattc aatataatgt taagattgaa gatggtatac gagattatga agatgtaata
     3001 tcttgggctg atttagtgat ttgcacagga agtacattat gtaataactc aattataaat
     3061 tttctatcac ttaacaaacc agtttactat tatggaacta cgatagctgg tgcatcaaat
     3121 attttaggtt taaaaagact ctgtttttgt agtaaataag caatcatatc atcaaggagt
     3181 acacaaatat atatgtgtac tctttaaatt taaaaagtta ttacttttat tctaagtact
     3241 aaagtaaaat tctattaaaa tgaatcccga ataaatgtac aatcccttta aatctcaatt
     3301 cctatcaatc attaatattt tgattagtct tatacataaa tgttttcttt attttaaact
     3361 aataaaaaaa ttttattcca ttaatctact ctatattttt cagcatttaa gaaataattt
     3421 ttagtacttt gtttgataat ataactaaga caacttttca ggagatgatt aaatgacatt
     3481 taagaataaa aaatttacta taaataaata tgaaattcta tctattattc tagttttatg
     3541 tttgatttta accgctttta gtagctatgt gaattatagt cataaaaaat ttaaagatta
     3601 tgttgtcttc tatgttcagc atcaagatga tgaagtgttg tgggctggga gtgctattgt
     3661 taatgctata aaagaaagag gaaaaaatca tgtatttgtg gttttagtat ctactggatg
     3721 tggtatatcc atctttgata aatataaaaa atatgaaaac ctaacaaata tacaaaaatc
     3781 agaatataga aatagagaat ttttagcttc tcttaatagc cttggtgtaa taagggaaaa
     3841 tataatatta ttaccagaaa caaatacaaa tggtaatact gacttcaatt acatggaaaa
     3901 aattgctctt acatttgaag aaaaatttaa aaatgtaact catatagcac atacttataa
     3961 gttagatgac catttacaac atttaaaaaa tggttctgtt atccaaaatt tatataatgc
     4021 tggtaaaata aaagatgcta aatattttgt aaaacctaaa tttgctgata aaataacttt
     4081 taataataag atagtttata aagctcattc tactgaagac tatgaaaagg ttaaaaatgc
     4141 ctgtcaacaa tacaaaattg ttgatgaatt agaatataga gaaggtattg gctataagtc
     4201 tgaccataaa tcatttgata agttattaaa taataaagat gttcctgcta tacttcatac
     4261 acctaattta taagaaatat ttattctttt aatttaagtt ataaaaagga aattatttga
     4321 gattttgtga tatagcactt tttgtgctat actacttttt ctcatacaac tattacccat
     4381 atctaatttc atcatctctt aaaatatata aagatatcat cattaaaacc tataagcctt
     4441 attaaatgct ttatacatac ctacattaaa ctacctgttt atattaaact acattaaaat
     4501 aaatatttgt tataaattct taataattat gaaactcatt tgttatttat atttgttaat
     4561 atatgtttaa acctaaaaaa ttaataaacc aaaataacat aagaagtgtg atagattacc
     4621 ctagatattt tcataaactt ttaccttcag attaatatgg gcgtctatac ttttaaattt
     4681 actatatgca ttgacaatat attaaaagta ctagttttta tttcataatg aatgggctta
     4741 ctgttttcta atacctcttt ttggtataaa ataaaaattg gttaaaaata aaatttcaag
     4801 ggggttaata agttgaatca aaatatattg gttgtagatg atgatagaga gatagttgat
     4861 gctatagagt tctatttgag acctgataac tttaatgttt taaaggcata tgatggactt
     4921 gaagcattgg acatactaat cgaaaatgat attaagttaa taataatgga tgtaatgatg
     4981 cccaatttag atgggcttaa aactacactt aaaataagag aaaaaaggaa tataccaata
     5041 attctcttat ctgcaaaatc agaagatatg gacaaaatta taggtcttaa tatgggtgct
     5101 gatgactata taactaaacc atttaatcca ctagaactta ctgctcgagt aaaatctcaa
     5161 cttagaaggt atataaattt aggtgatttt agtaatacaa ataactcaaa tgatactaat
     5221 tatattctaa aaagtggtgg cctagagcta aatacagata caaaaatagt ttctttagat
     5281 ggtgaggaaa ttaaaatgac accacttgaa tataaaatac ttacacttct tatgtcacga
     5341 aaaggtaaaa ttttctcttc tcatgagatt tatgaaaatg tctggaatga ggatgcttat
     5401 agttgtgaga gaactattgc tgttcatatt agaagaatta gagaaaagat tgaaattaat
     5461 ccaaaagaac caaaatattt aaaggtggtg tggggcattg gttacaaaat tgaaaaactt
     5521 aattattaat gattctaaag caaaatttat tttattatta ttactgtttc tattagtttc
     5581 tatattattt gaagcagttt atgatttgaa taataacttt tctaatatta attattatta
     5641 tagagagttt gctctccctt atttttattt tttcggtact cctgtacatt tgtcaaaagt
     5701 agaattagag tttgtctgca taggtattat attatcaagt tttttattga ctatactatg
     5761 ttttacattg tataaaaaaa ataaacaaac taaagtcaaa ttcttagaga atgtcataag
     5821 ccttttttct aaggtaaaaa tagaacttac acttatatta actttgatac tattaacctt
     5881 aaggtttcat gcatttacaa gtaataaaat gttttacaac actaatttaa taatctacaa
     5941 ttttataatt atatctatag tctatataat atatgaaaat attaaatata ataactataa
     6001 tctaaaagat atgtctataa cata
//
LOCUS       DAFKMX010000204         5913 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFKMX010000204 DAFKMX010000000
VERSION     DAFKMX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.207"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            49..1401
                     /locus_tag="LZB08_003284"
     CDS             49..1401
                     /locus_tag="LZB08_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBM7316749.1"
                     /translation="MTVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTG
                     YSNWRKFELDVTNLLKKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLN
                     ELTDINETFARVSADNFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLS
                     VSVYSDRASLAKSSIKNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQ
                     PKYNIKLDKFCGSEALVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIR
                     SLFDKGISPLPISVNFSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTD
                     TLFNVSRNLQDIGFIVAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIV
                     IKSIVSLIKQLGIRVVAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI
                     "
     gene            1434..4871
                     /locus_tag="LZB08_003285"
     CDS             1434..4871
                     /locus_tag="LZB08_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBM7316750.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4889..5635
                     /locus_tag="LZB08_003286"
     CDS             4889..5635
                     /locus_tag="LZB08_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBM7316751.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5584..5892)
                     /locus_tag="LZB08_003287"
     CDS             complement(5584..5892)
                     /locus_tag="LZB08_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBM7316752.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2461 a          586 c          910 g         1956 t
ORIGIN      
        1 tatgtaggta atattaatag agtgttagaa tatgaaacaa atgacataat aacagttaca
       61 attgctggat ggattatact ttcattttta tttttaggta ttataattta catattatat
      121 tcaaaagcaa atagtcaaaa aactatagaa aaagtagcat atacggattt tgtaacaggt
      181 tattccaact ggagaaagtt tgaattagat gttacaaatt tattaaagaa aacatcacaa
      241 aataataaat atgcaatggt catattcgat attgataaat ttaaagcaat taatgatata
      301 tatggccata aaaagggaaa tttaatatta aaagatattg cagatacttt aaatgaattg
      361 actgatatca atgaaacttt tgctcgtgta agtgctgata atttcaatat attattaaca
      421 tataacaaaa aagaggatat aataaatatt atcaaaaaga ttatggcaaa taatgaactt
      481 gtaaacttat cttttggaat ttatgaaatc aaagataagg atttatctgt aagtgtatat
      541 agtgatagag cttcacttgc aaaatcatca ataaaaaata atagtgatgt aaattttgca
      601 ttttttaatg acaaattaag agagaaatta ttatttgaag ataagataga aaaagaaatg
      661 gaatatgctc ttgaaagtgg gcaattcgta atgtatttac aaccaaaata caatattaaa
      721 ttagataaat tttgtggaag tgaagcccta gtcagatggc aatatacaga aaaagaggtc
      781 atatatccag gtgattttat tcctatattt gaaaaaaatg gatttattag aaagattgat
      841 atgtacatat tagaacaagc ttgtaaagaa atccggtctt tatttgataa aggaatttca
      901 cctttaccta tatctgtaaa cttctcaaga gttgattttt ttaaaaaaga ttttattgaa
      961 aacatagtaa atatatgtga tagatataaa ataccatata gtttaataga aattgaaatt
     1021 actgaatcta gtatgtttgg agatactgat actctattta atgttagtag aaacctacag
     1081 gatattggct ttatagttgc aatggatgat tttggctctg gatattcttc tgtaaatatg
     1141 cttaaaaata taccattaaa tgtaataaaa ttagatagag gattttttgt tgatgataaa
     1201 gatgttgata agagtcaaat tgttataaaa agcatagttt ctctcattaa gcaattagga
     1261 ataagggttg ttgctgaagg aattgaaaca agaagtcaaa tagaaatgtt aaaaaaagca
     1321 aattgtgata ttgttcaagg atattatttt tcaaaaccat taccaattaa agaatttgaa
     1381 aaattagtct ataaaatata gtcagcgagt cttaaatggg aggtggtgat taagtgataa
     1441 gagcaaaatc gttaaaaaat gtactgagta ttgttttaat atttttattt acaataattt
     1501 ctttttctga ttttgcattt aaaactaatg tccaggaaga agaaaatagt aaaattaatt
     1561 taagagaaat atccctacaa agttccaatg ctataaaaat aaaattagat agttctctat
     1621 cctttattaa aagtgtggca aatatggttt cttattttaa tactatagat aactcaaaaa
     1681 tagttccatt attagctaat atatcaaaag aatctgactt tcaaagaatc tctattgtat
     1741 ctcctgatgg aataagtcat accagtgatg gcaaaattat caatgtgtca aagtatcagt
     1801 attttaagaa agctattaaa ggagaaagta atatatctga agttgtgaaa tctccaattg
     1861 atggaaaaga tacttttgtt atagctactc ctgtatttaa taagaataac aaaataattg
     1921 gtgttttata cggttcttac tacaatgata aattagctga atttatagat ataacaagtt
     1981 ttaatagtga agggtacata gatatatttg aatctagtgg tcaatttgtt ttaaaatctt
     2041 cacatacaaa caaactgatt aagtcaagta gtaacatata tgatatattt aaaaaagctt
     2101 cttttgatga tggttatagc tataaaaaaa tatatgaaga tacaaaaaat aacaagagtg
     2161 gctttacctc atatgaatac aaaggagaaa atcgttatgc tacttataca cccattggtg
     2221 taaatgactg gtatgtaatt tctgtgatac cacaatatac tgtaagaaga aacattgata
     2281 aaataaatca attagcattt tctcttattt tcaaggtatt cttattgttt attatgctta
     2341 taacatatat tatttataaa aacaataaat ctcagaaaga tatactaaaa gctaacaaag
     2401 aaatctcatc tattacaaat agcattcctg gaggagtaca aaagtgtacc ctgggtgaaa
     2461 catgcaatat taatttttta agtgatggat ttatagagtt gtttggttat acaagagaag
     2521 aaataaaaac attgtttaat aatgattttt atagtttaat acataaagat gatgttgaaa
     2581 gaataaaaaa agaaattttt aaacaacttt acaaaaataa aactgttgaa ttagaatata
     2641 ggattataac aaaaaatggt aatacagtat gggttttaga taaaggggag cttataacag
     2701 aacctgatgg aaaagaatat ttttgttgtg taactgtaga tattactaga acaaaacaat
     2761 tagaacaaga gatggaacaa aaaaatctag aacttgaaat gatatcagaa aatgttttag
     2821 gtggtataat tataacagaa tttaatgatg attttactat aaaatacgct aactctggtt
     2881 atctttcaat gattggatat acacgtgaac aattagaaaa tgaacttaaa tacaaagctt
     2941 gtaatcttgt tttttatgaa gatagaaaga aggctcttga aagtatacaa catcaattaa
     3001 acacaaaagg aatagctgta tatcaacata gaataagaag aagagatggt aatattattt
     3061 gggtttctat aaaaggaagg cttattgttg atgatgatgg tattgaaaaa ggtatttgga
     3121 ttctaaatga tataacagat ttaaaaaatg cagaacaaaa acttaaaata aatgaagaaa
     3181 gatttagaat tgcattaaag caaacatcaa acactatatt tgagtatgat ataaaaacaa
     3241 agtcactttt aaatactagt aaagagcttg ataaatataa tatttataaa attactaaaa
     3301 atgttccata ttctctagta gaattaggag ttattcataa agattttagt gatgatttta
     3361 taaatatgta tgaaaatgta ataaatggag caaaaaaagc aagttgtgta gttaagatga
     3421 aacttccaga tgaaaattat atatggaata aagttacctt gactacgatt tttgatgaag
     3481 atggtaatcc tgtaagagca attggtatac aggaagatat aagcaaacaa aaggaagcag
     3541 agataagata caatcaagaa ggtaagtatc attcagctat attgtcggaa gcaatgataa
     3601 cttatgaaat aaatgtatca aaggatactt ttatcaaagg aaatgaaagt tggcaaaaac
     3661 catttggaat taagcctgac aataactatt ctcaaatggt aaaaactata gcaaatagta
     3721 ttgtacataa agacaataaa gaaacatttt taaatacatt tttatgtgaa aacttattgg
     3781 atgcatataa aaataatgtg actgaaataa aattagaata tcgaagaatg aatcaagaaa
     3841 ataaaatgat ttgggtcatt tgtacaatgc acttaataaa agattctatt acgaacgata
     3901 taaaagcact tgcttatgtt aaagatattg acaagcaaaa aaatgaagaa attagattaa
     3961 aattaaaagc tgaaagagat actcttacag gattatataa taaaggtact acagaaaaat
     4021 tgattaatga tttcttgaac tctgaggaag caagcttagg aatgcatgca tttttaattg
     4081 ttgatataga taattttaaa tctgtgaatg ataatttagg acatgtattt ggagataaag
     4141 tattatttga agtttctaaa aacctaaaac ctatatttag aaaagatgat atagtaggta
     4201 gaattggtgg agatgaattt atagtattct taaaaaatat tcagtctttt gagaatatca
     4261 aaaagaaagc agaaaaaata tgcgatatat ttagaaattc ttatacagaa aataaaaaga
     4321 catataaaat atctggaaca gttggaattt catttgtacc agaacatgga actgtatttg
     4381 aagaacttta taaaaaagca gacatagcac tttattatgc aaagagcaaa ggaaaagata
     4441 agtatgcact ttataatgat tgtatctacc aaaatgaact tgaaaataat ataagtgata
     4501 ataatataaa gttagcagat aaatgtgaga aatccaaacc agtacttaaa gaaattcttg
     4561 attctattga taattatatc tatatagtga atccaaatac ttatgaactt ctttttgtca
     4621 ataataaggt gtctcgtttg gatttagaga taaaattagg agaaacctgt tataaatcac
     4681 tattaaaatc tgatagacca tgtaataatt gccctcttaa cggattagaa gaaaataagc
     4741 attcaaaatt caaaatagag acaaatacat ttggtaaaaa tatcagtata gcaagttgga
     4801 ttaattgcat agatggagaa tacaactgtc ttatagagtg tatagattct tctttataca
     4861 aaaataaata aaaaaggact atgaagatat gttaaatagt ataactttga tacttggtat
     4921 aatatttaca atttactatt tttatctcaa aatagttttt ggaggaattt catttagtga
     4981 gattttctta gttgtaggaa ttgtactaat tatctatcaa atatttaaga aaaaaattaa
     5041 agaaaagaag gttttttata gggtgttaaa aataataatc agcatttttt taatagtatt
     5101 tgtagttact gagagtctaa ttatttttta tcccaaaaat agtttagaat caaaaagtga
     5161 ttatctctta attttaggag cttctgtaaa aaagactaca ccaagtacaa ccctgaaggg
     5221 taggttagac actgcattga aatacctaaa aattaatgat aattgttatg tggtagtatc
     5281 tggtggtaaa ggaagtggag aaaaaataac tgaggcaaaa gctatgaaag attatcttat
     5341 aaaaaatggt atagataaaa ataggataat agaagaggat aaatccacga atacttatga
     5401 gaattttaaa tattcaaaat tgaagataga agaacatagt caaagaaaac taagcgattt
     5461 aaaagtaaaa atagtaacaa cagattttca tgttctcaga agtaaaatat tagcatatag
     5521 aaatggatat aaaaatacgt ctttttatgc tagtaaatca aaactttcat ttgtaccaac
     5581 ttactataca agagaatttt ttgcattatg gaaaacaatc atttttgata gataataaat
     5641 aaaaagacta gatataaact catatctagt cttttctatt cccttaattc taatccattt
     5701 tccaacttta cttgttgtag aaatttcaat attgggattt acaagtaatt gcttataaga
     5761 agttttatat ttttcgattc caaaatataa ttttccttca taatccatta taaatccaaa
     5821 tggtcttact cttggtttat tatcttcaac tgtagcaaaa tagaatgttg agttttcttt
     5881 taaaaaattc attatttttt catgattaaa tcc
//
LOCUS       DAFKMX010000205         5912 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFKMX010000205 DAFKMX010000000
VERSION     DAFKMX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.208"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            116..1180
                     /locus_tag="LZB08_003288"
     CDS             116..1180
                     /locus_tag="LZB08_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316753.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINHLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(1308..2498)
                     /locus_tag="LZB08_003289"
     CDS             complement(1308..2498)
                     /locus_tag="LZB08_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316754.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2604..3983)
                     /locus_tag="LZB08_003290"
     CDS             complement(2604..3983)
                     /locus_tag="LZB08_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316755.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4014..5222)
                     /locus_tag="LZB08_003291"
     CDS             complement(4014..5222)
                     /locus_tag="LZB08_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316756.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         2019 a         1064 c          695 g         2134 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagtttgg tactgactta caaagttact atgatatgtt
      121 tctaaagctt atctttaaat ataatacatt agatttttcc agagcatact ttgacttaac
      181 agactgggca gataatccaa aagatatatc atattattta tcaaatggca catttggaga
      241 cttaatggat agtattgatg aaaatgcatc taaaatgagt ttatctgaca tagcaaaact
      301 tgaaaaaaag attgcaaaaa aacatattaa tctatggaat gaagggaaaa tcgatttttc
      361 tactactcgt tttagtagta ttgttcctat gttacaaaat gcaggagtaa actttcattt
      421 catataccct gatatagatt tattaaaaaa tacttttaat aacttaataa aagatattca
      481 tttacataga atgaagggaa atcaaccaag tgtaatatat ataacagtta aagatacaaa
      541 ttatgatgac tctactctag accaagttta taaaatatta aatagtatta aaaaagataa
      601 attaaccgat tttataattg aaaaaaatag taattctttt aaaattttca ctaaatgtga
      661 aacgattaaa tcattaacta aaaattttac tgcatgttat ataaaagaat ctattgaaaa
      721 tagtacaaat attaaagtat gtgttggata tggaattggt aatgatataa agcaagctat
      781 gtctagtgcc ataagtgcta ataaggaatc taaaataaat catttaaatt acagtttctt
      841 agttaacgaa aattatgaga caataggacc tttactgtgt gataagttac ttactgtttc
      901 aaataaagta actccacata taaatacaat tgccaaaagt tctaaattgt ctactcttac
      961 tattcaaaag attatatctg ttactgaaat tctacaaaga gatgaagtta ctcctaagga
     1021 attatcttca tgtttaaatg taacagttag aactgctaat agatttctaa gtagtctctt
     1081 aaagtctgga aacgctacta tattatatga aaaacaaaac tcaacaaaag gtagacctga
     1141 aagggtatac aaattattta tatcagaaaa taaatattaa aatccaaaat gcataaaaat
     1201 attttatcat ctaatattaa gtaaaattat atatgacttt ctaaaaggta tatctttgca
     1261 tctttttaag atataccttt tatgatattt aataatattt tatgttatta tctactaaaa
     1321 ttattttcta accattttac tgcacaatga gcaaataaag ctgaaccata aactaatccc
     1381 tcttcattaa aaattgcttt aggattatgc ataccaaaat tatatccctg actaatactt
     1441 cccattccaa tccataaaaa tactcctgga acccttagta aaatctctga aaaatcttct
     1501 gaacccattg cagatggaaa gtctattaca ttttcatttc ctataacttc tttaacataa
     1561 gatacaatct catctcctac atccaaatca tttataactg caggagtccc cattttaaat
     1621 tccactttag catttcctcc aaatgattta gctattcctg tagatatttc aataattcta
     1681 ttttttaatt tttctctaac aaattggtca aatgttctta aagttcctga catagatgct
     1741 ttatttggta tagcatttgg tacgtttcct gcattaaaca taccaatagt taaaactaca
     1801 ggttctttct gaggtttttc tcttgaaagt atagtgttta atgctatttg tatatgcgac
     1861 attatgttta ttgggtccac tccaaattca gggcttgctc catgagttgc ttttccttct
     1921 actgttattt caaatatatc cgcagatgcc atagctggac cacgtgaata agctacttga
     1981 cctgtgttaa gaaatttacc caccatactg tgaaatgcca tagctgcatc gacctgagga
     2041 ttttctaata taccatcatc aatcattgat tttgcacctc tggtagtctc ttcatctggt
     2101 tcaaatacaa actttactct acctttaagt aagtgtttat tatccataag tattttagct
     2161 gctcctaaga gcattgctgt gtgattatcg tgtccacaac tatgagatgc atctttattc
     2221 ttacttttaa aaggtaaatc cgtctcctcc ataataggaa gtgcatccat atctgccctt
     2281 aataataagg tctttccacc attttcactg tctaaaacag ctactattcc attattagac
     2341 gtcaatttcg gagaatatcc cattttttct aaagtactgg taacaaaggt tactgttttc
     2401 ggtatattgc aaccaatttc gggattttca tgtaaatatc ttctccatct aataatttcc
     2461 ccttctattg actgcgcttc tttaaaaaaa tttttcatat tccttcctcc taatcagata
     2521 gtcagcaaaa tctcactttc ttttagtaat gaaatgatgc tgatataaaa attataattt
     2581 tatatattaa atttcatgtc tttttaagga gttggaatat catctggaat aggacattta
     2641 tatgttttac cattcataga tttatcaaat tcttcttttg cctttttagc tatctctgga
     2701 ttatttaata ttttagctcc aaacatagcc ataatctttg atgcatatat catacctttt
     2761 tctcctatag aacttcctgc acaagatgta aactgccaac tatgacctgg tgctcctaga
     2821 ttcgtacacc ctgtaaaaaa gtatgctgtc ggaactatat gcattacatc tccaatatca
     2881 gtagaaccat aactattaaa gtttgtaaca tttcctccac cataatatag atgagttcct
     2941 tctggcaaat tatatttact tatcatagcc ttatattgtt gtgggctttg tctatctaat
     3001 tcagctgcaa aatccaattc ctctcgagtc caaggctctt gtttaatctc attcatacat
     3061 tctgatacaa gatttgctaa aacatgattg tttaaagtat tataacaacc tcctaaaaac
     3121 tctacttcaa cttcagtttc agtcatcatt gcagctcctt ttgctacctt tacaagtctt
     3181 tcatatgtac tttctacagc ttctctactt aaagctcttg tataatacca tactgatgct
     3241 ttatcaggaa ctatgttggg tgctacgcct ccatctgtaa ttgtataatg aatccttaca
     3301 tcagaagtaa catgttctct caaataatta gctcccacat tagtaagttc cacagcatct
     3361 agtgcacttc ttccattttg agggtctgag cctgcatgcg cagtctttcc cttaaagtga
     3421 aactttacag agttaacagc tgttgaagta ccaattgtag cttcactaat tttatttgga
     3481 tgaaaattta tagctaagtc taacccttca aacgctccac ctctagccat aaatccttta
     3541 cctgttaatt gttcttctcc tggacatgca taaaatacta tagtcccact taagttattt
     3601 tctatcatct ctcttttaag ccctactact gcccctacat gtgctgtaca taataaatta
     3661 tgcccacatc catgaccata tgcacccaat tcaaatgctt cttgctttgt tgaaactttt
     3721 tgatttaaac caggtaatgc atcaaattct cccataaaac ctataactgg tttacctgaa
     3781 ccaaaactag ctctaatagc cgtggctaaa cctcctacac ctacttccac atcaaatcct
     3841 tcacttctta gaaagtttgc tgtattttca catgctttaa attctttaaa tgctttttct
     3901 ggattttccc atattttctt acttaaatca tcaagtttga atcttttttt atctatctca
     3961 tttatagata tacttcctat cattttctat tcctcctata aatctattta ctatcaacca
     4021 aatataattg gacctattat actagcaaat gccactgatg caatggttac agtaacaaat
     4081 cctcctataa tcatctttgg taataaataa tctgttgccc tttgtttttc ttcttcagtt
     4141 ccttccatag catcaatacc ttcattggaa attatctgtg ttgcaggata agctagcata
     4201 caagttacac caattgcagc tgacattctt ggagaatatc ccaatagttt tcctacaatt
     4261 gcagtaaata atattataaa tacagaacct acaacaagta ttcctatagt tggaacaatc
     4321 atcccaaaca atgctgttgg agttacctta gcaagtcctc caggtaacat caacatacaa
     4381 ccaaacatta taattccatt agattgagat ttatctaaaa ttcctttttc taaaaaacct
     4441 attcgtgtaa aaatcaaccc tactaacaaa catgctatgt tgggatttaa ataatagttt
     4501 gcaggggtaa atcctggtat taaagtagcc aatcctacaa aattaccaat catagcaaca
     4561 aatgaaagct ttgcaagata cactaaagta gatgattttt tacctaaatc ttctttaaaa
     4621 attctcatac ttggaagttt aaaattactt tttgactcat ctttcaaaaa atccccatta
     4681 tctcttttaa attttaattc tttatttata caatatgtag ttatcggaat accaaagaac
     4741 ttttgaaatg ataatactag aattacaaat gcagcaagtt caggtctatt tgcagcttct
     4801 gcaacagttg ttacgataat cccagcaaca gtagaacctg ctattggagg tgctgctatt
     4861 aaagcatact ctctgccaaa taacatacta cccactgtta aacagccaac acctattcct
     4921 acaataccta ctaatgcaat tacaaccgtt ttccattcat gacataagtc ttcaagattt
     4981 ataagtgttc ctaagttagt tattaataaa gctataccaa agttgctcat tgcagctaat
     5041 atccctgatg ctcctgtaat gtcttttggt aaaatatttg tccaaaatcc tagtaaaaat
     5101 ataatacata caaataacaa tgttgaaatt gcaccttttg ttttcttaga taaaaactca
     5161 ctaaaagcat aaatcattac acatacagta aagcaagtaa gtggattcca aaaattttcc
     5221 attgttgttt cctcctaaat ataataattt tttatcaata aaacaataat tataaaatgt
     5281 atattatgca gttattctta actacatatg gtatagcttg atattttttg atgatattat
     5341 atacgtccta aattattcct aaaatatcta aataaatata actagagtta actctctaaa
     5401 tattaactat agttatcaac ttactagatt tctttttgat ttttactttc tacataattt
     5461 tacgagttag aattgtactt caactactct ctatattagt atacatgttg gaagacttat
     5521 atttatttaa atattaaata tagaatacat ttttataaac gtcttaaatt attcttaaaa
     5581 tatctaaaat gaaataataa tatacccatt aaaccaatat tatcagaata tacttcactt
     5641 taagtcttct ttatatagaa attttatcta ttagtgcatc ccctcctttg taaattagac
     5701 tatttctaaa cgaatttaga tatcttattc ctaggaacta ttactaaaaa caaaaattat
     5761 ttattatatt aaatcaatga taagccatac tttaaaattc tttctataca agtataattt
     5821 atcatataat gacatctata cctttatctt tatagtagtc ctaaattatt cttaaattta
     5881 caataaaaat aatttcacta tttataaaaa ta
//
LOCUS       DAFKMX010000206         5770 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFKMX010000206 DAFKMX010000000
VERSION     DAFKMX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.209"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..256
                     /locus_tag="LZB08_003292"
     CDS             <1..256
                     /locus_tag="LZB08_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBM7316757.1"
                     /translation="ERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVD
                     LSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            261..878
                     /locus_tag="LZB08_003293"
     CDS             261..878
                     /locus_tag="LZB08_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316758.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            1067..1411
                     /locus_tag="LZB08_003294"
     CDS             1067..1411
                     /locus_tag="LZB08_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBM7316759.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            1981..2847
                     /locus_tag="LZB08_003295"
     CDS             1981..2847
                     /locus_tag="LZB08_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBM7316760.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWKK"
     gene            2960..3364
                     /locus_tag="LZB08_003296"
     CDS             2960..3364
                     /locus_tag="LZB08_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBM7316761.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            3531..3980
                     /locus_tag="LZB08_003297"
     CDS             3531..3980
                     /locus_tag="LZB08_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316762.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNEIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(4136..4513)
                     /locus_tag="LZB08_003298"
     CDS             complement(4136..4513)
                     /locus_tag="LZB08_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7316763.1"
                     /translation="MLTPYLIFNGDCEKAFNFYAEAFGGGKTIFARLDSNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            4775..5692
                     /locus_tag="LZB08_003299"
     CDS             4775..5692
                     /locus_tag="LZB08_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBM7316764.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         2326 a          609 c          932 g         1903 t
ORIGIN      
        1 tgaaagaaat aaatataagt tatatattaa aaaaatattt ggttactcaa ctacagctag
       61 aagtatgttg ttaatagtct cattaatatt aacaaacatt attccaatag gcattatatc
      121 aacaagagta gatttaagta ataatattat cttatttgga cttctaatat tagttataga
      181 agtaattgta tctattgtat tagacaagat tatctcaaat agttcattta ataaaattat
      241 aaaaggagaa cattaaaaag atgatttcat taataaatat atctaaaaaa tttaaagata
      301 aatctataat agacaaattt aacctagaaa tttctaaggg cgagtttgta gcgataacag
      361 gaaatagtgg ttcaggtaag acaactttac ttaatataat gggattatta gaaaaaccta
      421 attctggtga tattataata aataatataa aaaatccaaa tagtaaacag ataagaattt
      481 tacaaaggga tttttatggc tatctatttc aaaattatgc tttaattgag aatgaaaatg
      541 ttgaaaataa cttgaaaatt gctttaaagt atcaaaaaaa tatcaaccat aatgaaaaaa
      601 taaattacgc acttgagtct gtaggtctta gaaaatacaa taaaaaaaag atatacgaat
      661 tgagtggagg agaacaacaa agggtagcat tagcaagagt aatattaaaa gaatgtgaag
      721 taatatttgc agatgaacct acaggtaatt tagataaaaa aaatagagac atagtatttg
      781 atatacttaa aagcctaaat aagaatggta aaactatcgt atttgtaact catgatttag
      841 agttatcgga gcaggcagat agagttataa aattgtaata atatcaacta taataaatat
      901 aagaaagaga ttacagctaa atcaagttta ataataatac gaatagaatt taaacattaa
      961 gtaaattagt agtttgtaaa tataagtata atatgatatc ataaaggctc aaaagaaatt
     1021 ttatataata agaaatctaa aaataacaaa tggaggacat ataaagatgg aaaatttacc
     1081 aaattgcccg aaatgtaatt cagaatatac ttatgaagat ggaactcttt tagtttgtcc
     1141 agaatgctct catgagtggg cattaggatt gggagctgaa gaagattcaa atattatcaa
     1201 agatgtaaat ggaaatgtat taaatgatgg agattctgtt acagtaatta gagaccttaa
     1261 agtaaaagga agttcatcat ctataaagat tggaacaaaa gtgaaaaata tccgtttaat
     1321 ccatgactca tcagatggac atgatataga atgtaagata gatggttttg gagctatgaa
     1381 gctaaagtct tctgttgtta aaaaagcgta atattataaa taagctgtag tgtttattgt
     1441 ttttcagtac agtataaaac cataagaatc tattaagtaa taaaagtttg tttatttatc
     1501 tttatatttt cttatggttt tattaaatat aaattttatt gaacaccaaa atattctctc
     1561 ctctacaaat aagccaatct tatttaaact aatatacttt gtactattta tactgaaatt
     1621 gtaaatttaa atgctatgtt atttaggttt gtatagttaa tttatatatt tctgatttta
     1681 taaattgtat ttattattat atcttgattc taaaatcatt aaaaatttca acttctataa
     1741 agatttacaa tttatcaaat acattatgag tatataaaca atttattatt acagtgctac
     1801 atttaaaata agtcttagta taaaggtatt gttacaagta taaagaaact tatgtatgct
     1861 attggaataa ataatacctt aaattataat taagaataag aactgttaaa taagggactt
     1921 gattatcatt tagttataga taatataata ttaaaaataa agtttaagag gagaaaaaat
     1981 atgatagata tacataaaaa tatatatgat aataagttat ttgaggagct aaagattgac
     2041 tgtaagaagt gctttggact ttgctgtgta gctctttatt tttctgcttc agatggattt
     2101 cctatcgata aggaatcagg aaaaccctgt attaatttac aaccagattt taaatgttct
     2161 gtacataata gtcttatgaa gcgaggtttt aaagggtgta ctgcatatga ttgttttggt
     2221 tcaggtcaaa aagtagcaca agtaacatat aaggggatag actggatgca atcaccagaa
     2281 ttaacaaatc aaatgtctga agtgttttta attatgagac aattacatga aatgttatgg
     2341 tatcttaaag aagcatctgt attaaatata tcagatacaa tcaaatcaaa aattgattta
     2401 ataattgaag aaactgaaaa gataactaat atgggcccag aacagataat taatttggat
     2461 atcatatcac atagaacaaa ggttaattta ttattatcac aagcgagtga gtctgtgatg
     2521 ggaaaggtta aatcatttat caagacttct actcttaaaa acatgaaaaa attaagtaaa
     2581 aatatagatt taataggagc agaccttaga aaaataaatc taataggagc agatttaaga
     2641 ggtagatttc taatagcagc aaatttaaga aatacagatt tgagtggggc aaatttaata
     2701 ggagcagact taagagattg tgatattaga ggtgctaatc ttgaaaatag tatattttta
     2761 acacaattac aagtaaatac tgcaaaagga gattctagta cgaagttacc agcttcacta
     2821 atacgcccta aatactggaa aaaataatta aattaataca taattttaca aaatatgaac
     2881 ttttttgact ttaattcata ttatacatta gagattcttt acaagaattt cttaatataa
     2941 tatagggaga gatataaaaa tgagttatca aaattataaa tgtgaaagag attatgtgta
     3001 tgatgattat aaatacaata agtgcaacaa gtgctataat gattatgagg aaatgacaca
     3061 tgttcatgaa tattcagaaa gtgttaagtt agcagaagaa tgtgaggata gacataacca
     3121 ccgtgcagca ggggttactg gtgaagctat accaataaat ggaggtacaa atcatgttca
     3181 taaaataaat gataatgtag atttccttga ccatttccat aagatatgtg ttacaacagg
     3241 gccagcaatt agaatacctg gaactgataa acacattcac ttaatatgtg gagaaactac
     3301 tgttaatgat ggtcattgtc ataaattcct ttttactact caaatagaag cacctttagt
     3361 ataagctgat ttgaattatt gtagtgaatt tgctagatta tagtataata atatataata
     3421 gataatattt aaacttaatg aggactatga tatttaacct tcgtagtcct ttctaagttt
     3481 tgtataaaaa agtgttatgc aattaaaagt gatataggga ggaaattttt atgaaatctg
     3541 gaataattct tgaaatgaaa caaataaatt caacagaata tgacgaaatg ttgcaagtct
     3601 gggaatcatc agtaagagca acgcatgatt ttttaacaga aaaagatatt gaatctttga
     3661 taccattagt tgagattgga cttaaagaag tagaaaatat agtttgtata aaagataatg
     3721 agataataaa gggctttata ggaatagata aagataaaat tgagatgctc tttatagaag
     3781 ataaatatag aggaaatggt attggtaaaa aattaataaa atatgctata gataaataca
     3841 atgtaaaata tgttgatgta aatgagcaga ataaaaaagc tgtaggattt tatattcact
     3901 taggatttaa agtgtttgat aggtctgaaa tagatggaca aggtaaccca tttccaatac
     3961 tacatatgaa attaatttaa agttatactt ctcacttatt cgcaaaaata tttacttaaa
     4021 tttttataca aataaaagtc gtgacagtct taggacacga cttttttact acactattaa
     4081 attgaaattt atctgttcta tattatgatt attagatgta gtttttttat ttttactatg
     4141 tacttaaata ccaagtatac ccatacctat caagtacttc agcagcgcca tcattttgat
     4201 gtggtggtgg gtgtggtaaa aattcttgta caagtgtacc accttcggca agttttacag
     4261 atatttcttc tatcgcttct cgagatggta gaacaacaca aatcgccatg ccagaaatta
     4321 caacaggctt gtctgtatcc gcacccatta tacaaccttc gtattttgtg aaagtaacac
     4381 ttgcgtgcat aacaggattg tttggattgc tgtctaatcg cgcaaatata gtttttcctc
     4441 ctccgaaagc ctcagcataa aaattaaatg ctttttcaca atcaccatta aatattaaat
     4501 atggagttag catgaccaat atatcctcct tgatatagaa taatttatag ttgcacctga
     4561 taatctaaat tatgatttag taaattgtat catagggaaa ccttgaagta tataattttt
     4621 tattattata ttatacaaat ttattaattt aaaaaaatgt tatatgtacg gaaggtttac
     4681 aggataaaat ataataaaaa gattaaggtg acattataaa aaatgtatat tgacattgac
     4741 gctacgtaat tgtatatact ttgtcttaaa ggagatggta aatatgaata ttatagaaat
     4801 tcgctcagat aaaatataca agaagataat ggatgcgcca ataaacaaaa aagaagatat
     4861 atacagatat gaattaatga agccttttga atttaagtgg aagtgtatga atgttccaat
     4921 agttgctaga cagaaaggtg gatatgatgt aattatagca agtgaaatgt taggggtttt
     4981 atcgcctaag gatattgatg aaaagcaaaa aaagaatata aatgtgttat ctgctgataa
     5041 aatttggggc acttgcaaag aaaccataga aaactctata aatgctttta taaaagaagg
     5101 gtatgattta aacattaagg actataaata ttcaatatta ttggcgaatc caaatagtcc
     5161 ttatacaata ttaagtgatg gatactgggg tgatggtggg attcctggat atatatttct
     5221 atcattggtt cctaatgaat atactatcaa tagattacca gtattaatag cacatgaatg
     5281 taatcacaat attagatttc agtttataga gtggaataat aatataacat tagaagaaat
     5341 gatgataaat gaaggtcttg cagaaaattt tgcaacatgg atgtttggag aggaaatgtt
     5401 aggaccttgg gtcagtagaa cagatatcga aacattaaat acttatataa agccaataat
     5461 aaaaagtgct ttaaaagaaa ctggatttca aaatataaca tcttatcttt atggtgatga
     5521 tatagctaaa atgcaaggat attttccagt agggttgcct tattgtgcag gatatgcttg
     5581 tggatattat atgattaagt attatttaga aaagacaaat aaatcaataa tcgaagcgac
     5641 tttattgcct tatagtgaga taatcgaagc agtaaaagag ttttgggaat aaattttata
     5701 aattgcataa tttgaaataa attatgcaat tattttattt ttaagaaaaa ataaattgac
     5761 aataatttat
//
LOCUS       DAFKMX010000207         5759 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFKMX010000207 DAFKMX010000000
VERSION     DAFKMX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.210"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            179..1630
                     /locus_tag="LZB08_003300"
     CDS             179..1630
                     /locus_tag="LZB08_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBM7316765.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1852..2313)
                     /locus_tag="LZB08_003301"
     CDS             complement(1852..2313)
                     /locus_tag="LZB08_003301"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBM7316766.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2418..3611)
                     /locus_tag="LZB08_003302"
     CDS             complement(2418..3611)
                     /locus_tag="LZB08_003302"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBM7316767.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3634..4299)
                     /locus_tag="LZB08_003303"
     CDS             complement(3634..4299)
                     /locus_tag="LZB08_003303"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBM7316768.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4333..5445)
                     /gene="ribD"
                     /locus_tag="LZB08_003304"
     CDS             complement(4333..5445)
                     /gene="ribD"
                     /locus_tag="LZB08_003304"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBM7316769.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5577..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1898 a          855 c          794 g         2212 t
ORIGIN      
        1 aaggtttgat ttgaatataa aactgcaata tattaaaaaa attattcatt ttaaaccttg
       61 taatttgatt ttataaatat tataattaaa ttaatgaatt tttaacaagg aattattcat
      121 ttttttgatt ttaagattta ttgagaaaac aaaactaaat aggggggtaa ttaatataat
      181 ggattttttc aatggtattt taacgagtat taacaatgca gtagtatatg taaacaatta
      241 cttatggtcg tatatcttaa ttataatgtt agttgtaatt ggtatttact ttactattaa
      301 aacaaatttt gtacaattca gatattttat agaaatgttt agacttctgg gtgatggtac
      361 agcgaataaa aatgcaaaaa aggaaggaaa aatctcttca tttcaagcat tttgtataag
      421 tacatcttca agagttggaa ctggaaatat agctggaata gcaattgcag tggtagcagg
      481 aggacctggt gctgtatttt ggatgtggct tatagcatta attggttctg catcaagctt
      541 tgttgaaagt acattagctc agatttacaa agtgaaagat ggtcaagctt ttagaggtgg
      601 accagcgtat tatatggaac aaggtctaaa taaaaaatgg atgggtattt tattctcaat
      661 acttataact atatgttatg gttttgtatt taatgcagtt caagcaaata ctgtttcact
      721 tgcatttaat aatgcatttg gaataagtaa aatggctatg ggaataggtc ttggaatttt
      781 aactgcaata gttatttttg gtggagtgca tagagttgca aaagtttctg aaataatagt
      841 tcctgtattt gcaggattat atatattagt tgctttggct atagttgtaa tgaatataac
      901 agagatacca agtgttatag cattgatagt tgaaagtgca tttgatttta aaggaatggc
      961 gattggtaca tttatgggag ttgtaatgac tggagtgaag agaggtctat tttctaatga
     1021 agcaggtatg ggtagtgctc ctaatgctgc tgcaacagca catgtaactc atcctgtaaa
     1081 acaaggttta attcaatctt taggtgtttt tacagatact ataataatat gtagttgtac
     1141 agcattcatg gtattattat attcaggtta tagtagtagt ggagcaactg gaattgaatt
     1201 agctcaagaa gctttaactc atcatatagg accagttgga aatatattta tagcagtttg
     1261 tatattcttg tttgcattta gttctatagt tggtaattac tattatggtg aatcaaatat
     1321 ggagtttatg agtggaagta agactaaact taatgtattt agagtttttg ttgtaggtat
     1381 ggttttattt ggttcactta ctcaagtaga tgtagtttgg aatcttgcag acttatttat
     1441 ggcattgatg gcgataataa acttaatagc aatagcactt ttaggaaaat atgcattcat
     1501 tgctttaaaa gattatacaa gtcaaaaaaa atcaggaata aaagaccctg tgtttattgc
     1561 agatgaaata gaaggcctag aaaaggtatc agagtggcat agggaattat ctgaggaaca
     1621 tttaggataa aaaatagtat aaaattataa aaccattttg aaagactaga gtatgtatat
     1681 actttatatg aatataatat tgaattaatt tatattactg gaatttttaa ttccagtaat
     1741 atttttttat tatatataat ttagctttat taaaagtttt tattattaaa aatagcttat
     1801 ttatcgtaaa gtgaatgata ttgagttttt aattattatg aatgttagta tttaatttaa
     1861 agacttaaat aaatttgcca tttctagcgc attcatagca caatcataac ctttatttcc
     1921 agcttttgta cctgctcttt caatagcttg ttcaatattt tctgtagtta cgattccaaa
     1981 tataacaggt aattcactgt ctaaagatac tttggctatc cctttagaga cttcactaca
     2041 tacataatca taatgagaag tagcccctct tataacacag cctaaacaga ttatagcatc
     2101 atacttacca cttttagcca ttttttgagc aacaagagga atctcaaaag ctccaggtac
     2161 ccaaactatt tctatatttt ctggagaaac gtcatgtctt aaaagacagt cttcagctcc
     2221 tgataaaagt tttgatgtta taaattcatt aaaacgagaa tttattatac ctatttttaa
     2281 atcttttcca ataagttttc cttcatatat catttttatt tcctccaatt ttatttatgt
     2341 tatagattta tatttataaa tatctttaat ttaattataa aaaactactt agtataaaaa
     2401 tatcatcata taatcattta tataatatct aaaagatgtc ccattttatc ttttttagtt
     2461 tttaaatata gcttattaaa gtcattaatt tcttcattaa tagggattct attttcgatt
     2521 tttattccat atttctctaa ttgatttatt ttatctgggt tattacttaa aagatttata
     2581 ctttttatgt ttaaattttt taatatttga gcagccatat agaaatctct catatcttct
     2641 tcaaatccaa gcattaaatt tgcttcaaca gtatcgtaac cttcttcttg aagtttataa
     2701 gccttaattt tatttaaaag accaatacct ctaccttctt gcttcatata aattaaaact
     2761 ccacttccat tttccacaat tttcttcata gaagattcta gctgtaatcc acaatcacat
     2821 tttagagagt ggaacacatc tcctgttaaa cattcagaat gaagtctaac tagagtattt
     2881 tctatattta tatttccata agtaagggca atgtgttctt catttgaata agtatctcta
     2941 tatcctatga tttcaaaatt tccatatttt gtcggaagga aagcactaga tactttttct
     3001 atagtagttt catttatttt gcggtattct ataaggtctt ttatagtgat gatttttaaa
     3061 ttatgctttt ctttgaaaat cattaaatca tcaactcttg ccattgtacc atcatctttc
     3121 attatttcac agataagacc aatctcttta aaacctgcaa gtctagcaag gtctactgag
     3181 gcttctgtat gtccatccct gacaagaact ccaccagttt ttgctattaa aggaaagatg
     3241 tgaccaggcg ttttgaaatc tttacttgta gaattttcat cacagaataa ttttacagtt
     3301 tcagctcttt catatgcaga tataccagtt agagtatctg ctccatctat agtaacagta
     3361 aaggctgttt gaaatgtatc tgtattttct ctaaccataa gaggtatatt aagactttta
     3421 aatttttttt cagtggcagg tagacaaatt agacctctac catatgtagc cataaaattt
     3481 attgattcag gagtagccat ttcagctgcc atcaataaat ctccttcatt ctctctactt
     3541 tcatcatcaa ctacgattac cattttccca ttttttatat cttcaatagc ttcttcaata
     3601 gtattaaaca tgtaattcac tcctttatta tttttaaaaa ccatttttaa ataaaaaatc
     3661 tttatctata tttgatttat ggttactact attagaattt aattccttgt agttattagc
     3721 cataaaatta ttaacatact ttccaattac atcgctttct aaattaacga aatctccaac
     3781 attctttgtc aagagaatag tgttaatttt agtatgaggt ataatagaaa ccttaaatat
     3841 ttcatcatct aaatatgata tagttaaact tacaccatca atagcaacag aacctttgag
     3901 tatcattgag gataaaagtt ttttatcagg tcgtatactt actaaaactg cattttcatc
     3961 tttttcaaat ttgcagatag ttcctttacc atcaacatga cctgttacta tatgaccacc
     4021 aaatcttgaa gaaagactca tagctctttc aagatttacc ttgtcatttg catttaataa
     4081 acctaaattt gtggaacgaa ttgtctccat cataacatcg gctgtaaatt cattttttcc
     4141 gaggtttgaa acagttagac aaactccatt tacggctata ctatctccaa gatttgtgcc
     4201 atctaatatt ttatttgata gtatggttac ttttccactc ttaccattag cagtaatttt
     4261 tctaagtatt ccaacttctt ctactatgcc agtaaacatc ctaacctcct atatgaatgt
     4321 attaacaaaa atttaattta aaacatctga ttctataaga atatcgccat ctatcaaagt
     4381 tacattattg attgttaact gtgttgaatc tgacaaatga tttattcctt ctccagaaac
     4441 gaatgtaggt gcatctttac cacctataat cttgggtgat agaaataatt tgactttatc
     4501 aactaactta tttctaaaga gactgtcatt gagagtagca ccaccttcaa ctagaacaga
     4561 atcaatgttt aactctccta atttatacat tacaaactcc aaatctacta agttgttttt
     4621 tgaaggagaa attattgttt caacattatt ttcttttaac atagataatt ttatactttc
     4681 tttgcaacat gtgaaaacta tagttttact ggttttatct ttaaccaaat tactattaag
     4741 tggcaacttt aggtgtgtat ctattactat tcttgtagga tgagacactt tttcttgatt
     4801 gagcctacaa gttagtaaag gattatcgtt caaaactgta tttatgccaa ccattatact
     4861 ttggtattta tttctgtatt tatgagttag ataccttgat gattcattag atatccattt
     4921 tgattctagt gattttgtgg caattttacc atctagactt actgctgatt ttacaataca
     4981 aagaggtctt ttgtttttta tatgataaaa aaatgcctca tttaaatctc ttgcttcatc
     5041 ttctaatatt cccacagtaa catcaatatt gttatctttt aattttttta ctccattacc
     5101 acaaacaagt ggatttgggt ctaatgtaga tattacaact ctcttaatct tattttgtat
     5161 aattttatct acacaaggag gcgttttccc ataatgagaa catggttcta agtttacata
     5221 aagagtagag tcttttagac tttgtttagc actgtttata gcatttactt cagcatgatt
     5281 agagccaaat ttctcatgat atcctttgcc tattatatta ctatctttaa caattacaca
     5341 tccaactaat ggatttggat ttacaaagcc ttctccattt ttagccaatt caatagcttt
     5401 tttcatataa taaatatctt tttctttttg attcacaaaa ttcacaatat gtacacctcc
     5461 tatttaattg gataattttt aaacaaaaaa ccctgagaat taactcaggg tttagaataa
     5521 tctacaataa atgtttaata aatgtagaac aaaattgctc tacaaaaaca ctcattaaat
     5581 atcttcttcc atccagactt tactgtcggc tttggaattt aaccaaatca accaagtttt
     5641 accttggctc gcgggctata ccgccgattg ggaatttcac cctaccccga agattaagta
     5701 ttaaatttat atttagaatg tatcataaat tattttactt tgcaaactat ttttttaaa
//
LOCUS       DAFKMX010000208         5731 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFKMX010000208 DAFKMX010000000
VERSION     DAFKMX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.211"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            133..516
                     /locus_tag="LZB08_003305"
     CDS             133..516
                     /locus_tag="LZB08_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316770.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            713..1258
                     /locus_tag="LZB08_003306"
     CDS             713..1258
                     /locus_tag="LZB08_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316771.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            1272..1958
                     /locus_tag="LZB08_003307"
     CDS             1272..1958
                     /locus_tag="LZB08_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBM7316772.1"
                     /translation="MNTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(2082..2702)
                     /locus_tag="LZB08_003308"
     CDS             complement(2082..2702)
                     /locus_tag="LZB08_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316773.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(2692..3714)
                     /locus_tag="LZB08_003309"
     CDS             complement(2692..3714)
                     /locus_tag="LZB08_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBM7316774.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(3715..4353)
                     /locus_tag="LZB08_003310"
     CDS             complement(3715..4353)
                     /locus_tag="LZB08_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBM7316775.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(4471..5622)
                     /locus_tag="LZB08_003311"
     CDS             complement(4471..5622)
                     /locus_tag="LZB08_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316776.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRSHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTKDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVNFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         1964 a          890 c          625 g         2252 t
ORIGIN      
        1 agtcatatat gaaaaaacta tattatacta tcttttcttt ttggaataaa ctatacttta
       61 aatatagata tttaatttaa ataaatttta cagtattaat agtaaattat cttagaaagg
      121 aatgtgcttt atatgtctac agacaaagtt gttggaatca tagatgaaga aacggcagaa
      181 cttgcaggaa ttgaatatac tggtaaaatt tatgcttctt ctggtgttat aaaacatata
      241 aagaaaaaac atagatgtca actgagtaag gatattttta atgatataat agacactata
      301 aaaatggtat taaagtcacc tgaatatata ggaagtcatc ctaaaaaacc tggaaaaagt
      361 gtggagttta taaaaaaaat tgatgattat attttagtag ctacagaatt agatacaaaa
      421 aaaggatatt tatatgtatc ttcaatgtat gctataaaag aagcaaaatt agaaagtaga
      481 gtaaacagtg gaagagtaaa acaatatgat ggttaacata atattattaa ttttccctaa
      541 aatattgaaa aatattatta tataagttat aatattaata tacacgtaga tatttattag
      601 ttagatttga ggttggaaaa ggcacccaat acgctactat agagtagtta atagagatgc
      661 aggatacgcc gccctgccaa atctaattaa attaaaaaga gaggatattt atatgaatga
      721 tttaaattta ggggaaaaaa tagctgaagt tcgaaaaaaa caaaacttaa gtattcgaga
      781 tttagcaaaa ctagctgatg ttacaccatc attattgagc caaatagaac gaggacttgc
      841 taatccatct gtaaattcat taaaatctat agcctcttct ctaaatgttc ctttatttac
      901 attttttgta tctgaagtag ataaaaaaaa cttgatagta agacatgata atagaaaaaa
      961 agttatttta cctggaagca aagaagttat atatgagatt ttaattccag actctagtgg
     1021 taatttagaa tttgcaatta tggatttagc tccaaacact tcttcttgtg ttgataggat
     1081 tactcataat ggagatgaaa ttgcatatgt tttggaggga gaagttaaac tttttatgga
     1141 tgatgatgaa tttactttgt ccaaaggaga tagtgtaaaa gttcctttag gaacaaaaca
     1201 taaatggcaa aatgactcaa atagtgaaag taaagtaatt tttgcagtca tcttgtaaag
     1261 gagtaaaaac catgaataca tataaattta tattttttga tgcagatgat actttatttg
     1321 attttaagaa aagtgaatca catgctttta aaaaacttct atctgagttt gatttagaat
     1381 ttaattttga gaattatata gagtcatata gaaatataag tgataagctt tggctcgacc
     1441 ttgaaaaaaa tattataact ttgaatgaac ttaaattact tagatttgaa ctttttgcaa
     1501 ataaaatatc tttggatgtt gattctgaaa ctcttagcaa aatgtactta aattttttag
     1561 gtgaatgtac atttttaatt ccaggtgcaa tcgatatatt acaatatctt aaaaaaaagt
     1621 atactattgt tataataaca aatggaattt ctgcagttca aaagaaaaga cttgaaaatt
     1681 caaaaataaa aggatatatt gatggaatgg ttgtatctga agagttgaaa atatcaaaac
     1741 caaatcctga aatatttaaa tacgctctca aaaagtttaa ttgtcatgat aaatcttctg
     1801 ccttaataat tggggatagc ttaacctctg atgtcttagg tggaataaat tcaggaatag
     1861 atacttgttg gttaaattcc aataacagta taaattatac taatcatata ccaacttatg
     1921 aaataaatac acttattgaa ttaaagaaac tactatagtt tgtaagtctg ttcaaattaa
     1981 atagaatatt actttgcaat aattttttta gtttatacat aaaatgacca aaaaaattat
     2041 tgcaattcat acacaaaaca aatcttagtc ttgaaaacta gttaaaagca ttttctagga
     2101 catttgctac atcttgattc taaattttct tcatccccca ttaaaataag cattgcaaca
     2161 ctttttatgg gggatagttg atgataatca ttgatttcta tttctatact attatattta
     2221 ttttttacat agttataaat atccttttgt ctctctactt caaaatcttt atctcctgga
     2281 tatatttcat gcgatatttt gagactatca tactttcctt ttaaatacat ttttattttc
     2341 tcattaatat aatctaaaca tactatacct attttatcca atgctagccc tgccatcata
     2401 ttgcaatttt ccatatagtc attcatctta atatcaattt tatcccctat tgtatatagt
     2461 acagcaaatg cttttttaca ttctttaggt gttttattaa tatcaacttc acttatatat
     2521 atcttgatat ctaaaatatc atttatattt cttatttctc tttcaacgat tttactaatt
     2581 gtatctggag cttttctatt tccatacccc aaaaacttta gcacaatatt cttatctata
     2641 aaaatatcat ctaaatttat atcctttaaa agatatttat ttttattact attataattc
     2701 atcaatatca atcctatcta attctttata actaattctt cctgccttta tagctgcttg
     2761 taaattttct actggagtgg tatttaagca gttacagcca aaatcacata aaaagttatc
     2821 atatcttctc atcttcttta acaattttag cgtctctctt ctaatcttat ctggcttaga
     2881 acgtccaaga agttttattg ggtctaaatt accaatcaaa actatatctc taggaatcat
     2941 tggagcaatt tcttcatagt ctaaaatttg gtctaaactt actgcatcta tattacaatc
     3001 taacatatta tctaataatt ctctggtgtc tccacatata tgcatacctt tccaacaatc
     3061 aagttcattg tagattttat ttaaattttt tactataaat tcatcaaatc tatctttact
     3121 tagagttact gatgttggct ctgctattga aatatatttt gctcctgctt tatttgcagc
     3181 tacagcatat cttcttacca attcagttgt aaattccaat aattcttcta caaatttttt
     3241 atcagttatt atacatctta aaagttgagt agctccagct aattgacctg caagtgtaaa
     3301 tggaccttgt atcgattcat atagtggttt tttaatactt tttgatataa ctgataagct
     3361 ctctatattg agtggcatac gtccactcgt ataagggtct ggaacatcta attttcttaa
     3421 tttatctctg tcagttatag tatgtgtcaa tacacttgga aaatcataat ctggtcttaa
     3481 tgtctctaaa cccagagttt cacaaaatgt aatcccatca cagaatgagt atataaaatc
     3541 tgattcaaaa tacttgtcca tagtctttgc taattctaat tgcaaatcta cactatcata
     3601 tacttcatat gcctttttat ttattaagaa caatccattt gtacccatat caggcagaaa
     3661 aaaacctcta tcttcactat ttatatactg aagtaaagtc atatctgaaa acatttaaac
     3721 ttcacctcct ataagtttat ttattaattt aactgctttt ggagcatttg aactgtatcc
     3781 atctgctcct atttcttctg caaactccat acttacaggt cctccaccaa ttattacttt
     3841 tggtctttta tctatctcaa tcatattaag ttcgtctata accgatttca tattttccat
     3901 tgtagttgtc atcattgaag ataagcctat tatatctaca ttgtttttta tagcttcttc
     3961 tattatatct tcactttttc tatttacacc taagtcaata accttatatc cagtggcttc
     4021 taccattact tttacaatat tttttcctat ctcatgagta tctccttcta ctacagacat
     4081 cagaattact ccctttgatt ttttctttac ttttccagtt cccttcaaaa gattaatacc
     4141 tttatttaat gcatctgtgc atactataac ttctgaaaga tagtattcct tattctcaaa
     4201 cttatctaaa gctcctatca tacctttgtt taaaccatat tcataaatat cttccacatc
     4261 aatattttta tctatagcaa tttttactaa attctctata ttatctacac ccatatttaa
     4321 tatacattct gctatatcat caagtatgtc catataatac tcatcctttc taattactta
     4381 caaaattgct ttttcaatat ccaataattt ctcttattta acaaggacta actctaggta
     4441 tcaatcaatt ttatttaata tcatctaaaa ctattgataa tttaaatttg cagtccatgg
     4501 aaaatctaca ggaaatgcct ttgtagcaaa ttcatatata ttttttagat gtttaaattc
     4561 attgtactgt tcctcatttt tttcaaaaaa cagtttatta agcttatcca aattttctat
     4621 tacaagttct tcttctttgt atttaataat atatttattt tcaacttgtt taaattccat
     4681 gtattttagt aattcaaaat ttacatattg actaaaataa cttcttaaac tatcacacaa
     4741 cttagtaaaa tttattattt tcatagttcc ttgttgatag tccaaagatt ttgtcccatt
     4801 acatttcaac tggtctttaa gatttttcac atgtacttta taatttaaat attctaatcc
     4861 aaatttattt gcaacatatt tcaatatatt ttcaacattt acagagttta accctatttc
     4921 agcaacttct cctacagtag aatcttcttt ttttattgtt cttattatca aatatcctat
     4981 tatattatta ttttccttta taaatacctt cttgtaacca attggtcccc aggcaatagt
     5041 tgctgcatgc aatagttttt gaaactcatc tttcgttctt ataaatctag tactattttg
     5101 attatatagt tcaatcattt tatctaaatc attatctttt tcaaaattag tttcatcaaa
     5161 ttcaactatt tcatatgcta tttttacatc ttcaggtttt attgtatatt tataaaaact
     5221 ctttaccaaa ctacaatttc ttctactgta tagacttctt gttcctgata taattacaat
     5281 atctacacca tcataaaaca ttttttcttc tactttatcc aatatcttag atgaatagcc
     5341 tctcttttca taatctggat gagtacatac tccccctata gctgcaacct tcaatctgtt
     5401 tccttgaata gatacatctt gaattagata gtttacatca gaaaccactt tatcatcttc
     5461 tgatattata atcatatttt ctatattatt tttacttagt aaaagaggaa attcttccat
     5521 catagttggt ttatgacttc ttgatattct aaatacatag tttattaatt ctattacttt
     5581 atcaaattct tctaattttg cacttcttgg tcttaattcc attcatattc ctccaaatat
     5641 tatttaagat acttaaaact ctattatcta taataaaaca tttattacta cagttctaat
     5701 tatatctttt ttctttacaa tttttaattg t
//
LOCUS       DAFKMX010000209         5693 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFKMX010000209 DAFKMX010000000
VERSION     DAFKMX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.212"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            92..1417
                     /gene="cdeA"
                     /locus_tag="LZB08_003312"
     CDS             92..1417
                     /gene="cdeA"
                     /locus_tag="LZB08_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBM7316777.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1512..2306)
                     /locus_tag="LZB08_003313"
     CDS             complement(1512..2306)
                     /locus_tag="LZB08_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316778.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMSLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2307..3182)
                     /locus_tag="LZB08_003314"
     CDS             complement(2307..3182)
                     /locus_tag="LZB08_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316779.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3186..3554)
                     /locus_tag="LZB08_003315"
     CDS             complement(3186..3554)
                     /locus_tag="LZB08_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316780.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3737..4396)
                     /gene="deoC"
                     /locus_tag="LZB08_003316"
     CDS             complement(3737..4396)
                     /gene="deoC"
                     /locus_tag="LZB08_003316"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBM7316781.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4432..5619)
                     /gene="murJ"
                     /locus_tag="LZB08_003317"
     CDS             complement(4432..5619)
                     /gene="murJ"
                     /locus_tag="LZB08_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBM7316782.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         1902 a          899 c          629 g         2263 t
ORIGIN      
        1 agctgtttat cgtataaata aaatttttct tgactatata gttactatat agcttattat
       61 atatagagag taataaaagg aggtaaaatt tatggaaaat ttatttacaa gaaaattcac
      121 tacttttgaa tttctaaaat ttgtttctcc tgcaattata tccatgatat ttatatcttt
      181 gtacacaata atagatggca tctttgtatc gacattagtt ggttctgatg ctcttgctag
      241 tataaatatt gtactaccta taattaacct tgtttgtgga tttggaataa tgatggcaac
      301 tggtggaggt gctatcgttt ctatacgtat gggtgaaaat agacaggatg aagccaactc
      361 tacattttct tttatagttt tgttttcatt gattgttggg attttattca cagtaatctc
      421 atatttcttt atcaaagaaa tatctatttt gcttggtgca acagataagt tattaccata
      481 ttgtataact tatggtaaag ttatgatttt atgtacacca ttttatattt taaaatttat
      541 atttgagtac tttgcaagaa ctgatggaaa ttctaaattt agtttatttc tatcagtcat
      601 tggtggtgta acaaatataa tattggatta tgtatttatt aaatattttg gaatgggtct
      661 tttaggagct gcagttgcaa ctgctatagg tattatttta acttgtgttt taggtattat
      721 ttacttctta tctaataaat ctacactaaa attaagaaaa ccaaaaaccg attttagact
      781 tataagagat actatgataa acggttcttc tgaaatggtt acagaattat ctacaggaat
      841 tacaacattc ttatttaatg tagtagcttt aaaattagca ggagaaaatg gacttgctgc
      901 tcttactata gtattgtatg ctcatttttt aatgacatca gtctatctag gattcgctgc
      961 tggagtgtct ccattaataa gctataattt tggtgctgaa aacagtgata aattaaaaga
     1021 aacatttaaa cattctctaa aatttatatt tgtttcttct cttttagtgt ttattattgc
     1081 tttagtattt gcaccattta ttgttagggt ctttgtaagt ccagataaca cagtatttaa
     1141 actagcccta caaggattaa aaatatttgc atttgctttt ttgtttgttg gtataaatat
     1201 atttgcatca ggatttttta cagcatttca caatggaaaa atttcagcta ttatatcttt
     1261 tagtcgtgcc tttgttttta taatcatagg aatcataatt cttcctccta tgttaaacat
     1321 gactggatta tggcttacag ttccatttgc tgaggttata accatattta tatctattct
     1381 atttataaaa aaatataaat gtagatataa gtattaaaaa ttaatactta aaatcaacat
     1441 ttattaaaac taataaacct gctttaaaat acaaatttac aaatttgatt tttaagcagg
     1501 ttttatttta gttataataa gtcatttgca tactctttaa ttggtgcttt tctaagtaat
     1561 aaaattataa atattatgca aataactata attcctactt ttgttccaaa taaaataaaa
     1621 ggattctgaa atgaataaat aattttttct aaatacaata gcattttatg catattttta
     1681 gttgcaaaat taactatata aattaccatt aatatcggta atcctatcaa tatagtaata
     1741 ctagtaactt ttctaactct atatgtaaaa gctccaagta acactcctat aaaacctata
     1801 catatttcat ttatacatat ttgtacaaaa taatttaaat aagttagtga catacttgat
     1861 gttgaaggat ctatcagtgc tgatatagat gtccaaatca tatctctctt tagtaaaact
     1921 gatagttcta tactataacc tgttataatt tctataaata acttcgataa aaaaatcaaa
     1981 actataccta aaatagaaat cacaaagctt aaaataatta aatctatgac aatagctttt
     2041 atacaacttt ttctatctgc tcttatactt aaagctcctg aaaagtcttt atttgctata
     2101 tttactccat acacaaatag cagtatagtt atatatgtta aaataggatt cacaatcgtg
     2161 gataaattag ttttagatgc ttctccaaaa atcttgagaa taaacactgg caaaatattc
     2221 ataacaattc ccacaatcat aaatataatt atatacaatt tagtattttt attaaaaaat
     2281 tttatatatg gctttaattc attcaattac aattcctcct tcttagtcat gttaacaaac
     2341 atatcttgta gccctatgta tcctacatca atatttgaat ttttaagtat tttttcatca
     2401 ctttcactaa aatcaccata gtaaaaataa gcaactgcat ttccaaatgc ctttttaggt
     2461 tttatatttt ttatacattc taatttttct aactcttctt tatttccagt aatataatga
     2521 gctttttgtt ttatgacatc aatttcttca tctataataa tttttccctc atttaatatt
     2581 attacatgct ccagtaaatc atctatttca tcaatcaaat gagttgatat tattattgtc
     2641 cttggatttt ttatataact atctaaaatt atgttataaa actcttgtct atttactgca
     2701 tccaaaccaa ttgttggctc atcaaatatg gtgatagctg aattagaaca aatacctatt
     2761 atatttgaaa gtatagtttt catacctctt gacagttgtt tatacttttt tttagtattt
     2821 aaatcaaaat atttgcataa ttcttcttct aagcctttat cataactttt ataaaaacta
     2881 gaagaaaatt caaatatctg acctacttta aaatctggac tataaaattc tttttccctg
     2941 acaatacaaa cttcttccaa aacttttata tcttcttttg ggttctttcc taatatttct
     3001 atctcacctt cattttgaat taactgattt accattatat ttaataatgt tgtttttcca
     3061 gctccattct tacctaataa accatatatc ttattttttt caaagtttag agacatatta
     3121 ttaaaaatat tttgcttctt aaaactgtga gataaatttt ctatcttaat agaactactc
     3181 atatattaat cctccttgaa attaattata atatcaacca attcttctct atttatttct
     3241 aatttgttag cttcttgaag taggtttttt acaaaattat gtttaaaatt ttcttttcta
     3301 gcttctttta taatagtttt tgcaccatct gatacaaaca tccctatccc cctcttctta
     3361 tataaaatat ttttatcaac caatatattt atacctttta atactgtagc tggattaatt
     3421 ttataaagct ttgaaagctc tgttgttgat ggaacctgtt cttcttcttt tattccattt
     3481 gatagtatct catcttcaat tgctcgtgct atctgaataa atataggttc ttcattattt
     3541 aatactaatt tcataggttc ctcctttctc cattagttag ttactcaact aattaactat
     3601 ataacttata atatcttgaa aaatattttc tgtcaatact aaataaaaaa cagagggaaa
     3661 attccctctg ttttttgttc tttatattta taattaaatt ttaatttttg taatcctatc
     3721 ccataagtta aaaactttaa taatctgttg tatcttcatt ttttaatatt ttacatattg
     3781 agctagttcc taacctttct gctcctgcct ctataaattt ttctgcatca gaaattgatt
     3841 taactccccc tgcagctttt atttttacat tttttccaac atgctctttc ataagcttta
     3901 tatcttctaa agtagctcca cctgttccca ttccagtaga tgtttttata aaatctgaac
     3961 ctgacattgt tactatttca cacattttta ttttttcatc ttcatctaaa taacatgtct
     4021 ctataatgac ttttaatact ttatctccta tagctttttt tatttctttt atttcttttc
     4081 ctatattatc ataatcttta ttttttatat ctgaaatatt tacaaccata tcaacttcat
     4141 ctgctccatt ttcaatagca tcttttgctt caaatacttt tgtagcagta gtttgatatc
     4201 ccaaagggaa ccctatcact gtacatatct ttattttacc ttttagatat tctgatgctc
     4261 ttttcacata tgatggtggt atgcaaactg atgctacact catatctaca gcttcatcac
     4321 ataaaatttt aatatcttcc catgtagttg tagcttttaa aatagtatgg tctactgttt
     4381 ttaatatatg tttcataaaa tcctcctaaa tttataatta atataattag attacttttt
     4441 taaatgcaat tttctctttg caatgtttag tatcatatca acctcttcaa ctttaaaaat
     4501 aatcattagt agtgtataaa ttccacctcc aactataact gaaatagcta gtgatacaaa
     4561 ctcattaaat gttcctactc ccaaaattcc aaatataaat ttatacgtaa agtatgataa
     4621 aacacccata attaaagatg caactaccga tttcaaagtt gcttttatta ttttatcttg
     4681 tccaaaatat ccaacttttc tctttaggtt caagaagagt aacaatatac atgcaattga
     4741 tgatgaacta gttgcaagag caagaccacc atgagccatt ggttttatta aaactaagtc
     4801 taacactata tttaccccta cagatataat cccatttacc ataggtgttt ttgtgtcttg
     4861 taatgaataa aaaacctttc ccaaaatatc tcttaagcca aatgcagtca taccaactgc
     4921 ataaaaaatt aaggcagttg ctgtcatttg ggttgccctc gcatcaaaag cacctctctc
     4981 aaaaatgatt ctaacaacag gagttgcaaa aaaaatagaa gcaactgaaa taggaatcat
     5041 agaaattatt atcatattta tactggattt aactgaacta gtaaacttct tttgattatt
     5101 ttctgcactt aacttagata acataggata cataacagat actattgaag ctgtaaatgt
     5161 ccctgtaaca aaaccattta atctatctgc ataagtaagt gctggaatgc ttcctttaac
     5221 taatgttgag gctaaagttg tatctaccaa tgagttcact tgattaacag caacacctat
     5281 aaatactggt gaaagtaatg ctagtaattt aataaggcta tcatctttaa agtttaagta
     5341 gtataagtat ttgtagttcg tttttttaac aaaaaacatg taaaagagca gttgaactac
     5401 catagcaacc acagcaccaa ttggaagtat gtatggtcca aatatggtac ttaacattat
     5461 tgaaataatt attactatat tatagggtat acttccaaat ccaactacaa taaagttctc
     5521 tttaatttgt aaaaatgctg acataacact tgtaatacct atgaatatta ttcctgttct
     5581 gaacctccca tgactaaagt cacagggttc ctgcttcata gaattttgca tactaacata
     5641 tgtcttacta attctccaca ggctatcccc gtagttccta cggttcttac ata
//
LOCUS       DAFKMX010000210         5657 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFKMX010000210 DAFKMX010000000
VERSION     DAFKMX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.213"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(6..299)
                     /locus_tag="LZB08_003318"
     CDS             complement(6..299)
                     /locus_tag="LZB08_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316783.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(785..988)
                     /locus_tag="LZB08_003319"
     CDS             complement(785..988)
                     /locus_tag="LZB08_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316784.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(1039..1290)
                     /locus_tag="LZB08_003320"
     CDS             complement(1039..1290)
                     /locus_tag="LZB08_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBM7316785.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(1306..2376)
                     /locus_tag="LZB08_003321"
     CDS             complement(1306..2376)
                     /locus_tag="LZB08_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBM7316786.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(2405..3826)
                     /gene="ccpM"
                     /locus_tag="LZB08_003322"
     CDS             complement(2405..3826)
                     /gene="ccpM"
                     /locus_tag="LZB08_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBM7316787.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSENSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(3848..5620)
                     /locus_tag="LZB08_003323"
     CDS             complement(3848..5620)
                     /locus_tag="LZB08_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316788.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
BASE COUNT         1977 a          910 c          479 g         2291 t
ORIGIN      
        1 atactttaaa gattcccatc tttatcatat tttattctta taagaaaata taatcctatt
       61 actactaact ctatcactaa aaatactcca taggtaagaa ttgaatcaat actatctaac
      121 ccatttctaa aaaaaattaa tgctatacct gctgctataa tccctatagc attttttctt
      181 actgcatttt gcccaactcc ataaaaacct ggcatttttc cttttatttc gacagcagtt
      241 aaaattatcc caaacactat aaataatact ccaataagca tcataatcat actaatcatc
      301 taattacctc cttcacttaa ggaaatttca atttctaaaa tctaaaataa ataatattta
      361 ttttttataa taatattgta tatccaatta actattttgg aaattagtac caataaataa
      421 tgtttaaata ttaaaataat atattaattc taatcaatat tattaatata tatatacatt
      481 tttacaaata tatttaaaaa ctttactttt aattcttact atttaaataa gtataattta
      541 ttataataac ttttaattaa tttatacttt taaattgtaa aaatcacccc taatcactat
      601 atttaccact ataataattg aagaggtgat ttatataaca gatactattt atgcagttat
      661 tataaaatca cactcttaat tataatcaac tgcatatttt tataataaaa ggaaataaag
      721 taagaaagat aaatttaatc aaatatgcta aatcaattta atagtatact tattactgaa
      781 atatttacat tccatcagga gttctttggc catgtatcaa tttattataa tatgtattat
      841 atgatcctcc tcttgcatta taatcattat tcttgcagtt acaagatgaa gatgccatca
      901 tcttacaagt ataaggacac ataatagtac aaacacctct tgtctcaaca cctatattat
      961 tcatttctat tctttttcca aaactcattc atatcctccc cttttctata agatcactta
     1021 ctttatacct ttattaacct ataccatatt gattattttt agaacattat caaaactcgt
     1081 aaattgccta ttcaaaagtt tttcctctgg aatttttata ttaaagtttt ctattacctc
     1141 gcaaaatact aaaagtaatt ctcttggctg tatatgtaaa gacggtgaaa aaaataattc
     1201 tttttttaaa ttgttatttt tattaaaatt aattccagtc acatcaaaaa atatattctc
     1261 tagtagtaat tccttttcag tgtaattcat aattaacctc caattctaaa tagatattac
     1321 tttagcatac tcttttaatt gattcactat attgtccact aattctagta attgtttatt
     1381 atcatgtata aaaaatgttt tttcataatc ttctatatct tcaacaaaaa agttttcatc
     1441 taatgacata tatgaaaatt tttgcttcac ctctgtctca tatgtatcta taactttttt
     1501 ctctatacta aaatagtcta tatttatacc atgtcttcct ttaataaaat atttaagttc
     1561 ttcacaatat tctctatcat atatacttga tggaatacat aatataccac aatcatttat
     1621 tactgcatta gaaatttcaa aagctctaat tccaaaatta ccaaccattt ttctagagaa
     1681 agggaataat tctcctggaa cttctataat tattaaatct ggctttgcct ctacctcttt
     1741 atcttttata aaatggttaa aattaataat ctttgtctta ctacttatcg aatcatcaaa
     1801 cataaactct ggtataggaa tcattttaaa taaatagctc tcttttttag ggccaaattg
     1861 taatattgaa aacccccttt ttcttagttc ttctcctatc cttatattta catcaaaagt
     1921 atctaaccct ccataaagac cacaaataaa tattattggt gtgtttatct gtgatatacc
     1981 ctctatatct cttatttcat atttttctaa attccattca ttaatctttt tgaattcata
     2041 atgagatata tacctagata ttttattaag atcatcacgt gtatataata tctttttacc
     2101 ttcttttaga gctactttta atttaggaat tataaatttt tctaagtcta gattttcttt
     2161 tgaatcaaca aaccatacca aagaattctt tttaagttca ctttgaaaat catttgtaac
     2221 tttagtaata atattattaa cattatactc cttattgcat aacccccagc cagaaggact
     2281 aaccagtgaa ttaatttcta tatctgaaat tttattgcaa tgatttaaaa tagtaaaaaa
     2341 attataatta aatggatata taagtacttt ttccataatc ccatctttcc tttcattaga
     2401 aaatttaata ttgatatcct agagatttca aaacgcagta atctttcata ttattttctg
     2461 tagaatgtct aattctgtca caatcatgtg gttttatttc attttcatta catttagtat
     2521 catcaatctt agctatacat atatcacaat aactataggc ccaacaatca gaacattgct
     2581 cttcatataa ttttcctata tttaaaagtt tttcagcctt ttctatatct actccactat
     2641 ctaaatttcc aatcataccc attttagaat tttcacttac tctctcacat ggaaaaaatt
     2701 ctccatctgt agttacaaat attctaaaag ctcctggtat acatggtcca ccatgatgcc
     2761 acacatctgg taactctaat ctaccttcaa tatttttctc atatctaaaa tcatctaata
     2821 aggctctttc tattcttgat aaaggtgaca tactctcatc ttttataaat tttaatttgt
     2881 tgagtaaagt caagaatcta tcataactct tacctaatat atattcatct gatgaaacta
     2941 tttctttatc agaatagtta ttattaagag tggaagctaa aaagtttgaa tcttttaaag
     3001 tctcattttt agatataaaa tcatctatag aaatgtaatc attttctgaa tttatcactg
     3061 tattaaatgt aatattcttt ttataatatt ctggatattt attttttatg taacgtacat
     3121 tttttaatac aacatcatga gatccttctc ctgtatttgc aaattttcta cttttatcat
     3181 gaatttcttt tcctccatct aaactaatca tagttgttat attatttttt acaaagaatt
     3241 ctattatttc ctcattaaat aaagttgcat ttgtagttag attaaagtat atttcctttc
     3301 cttctccaac tacttttaca tattctacta tctgcttaat caaatcaaac tcaagtaagg
     3361 gttctcctcc ataaaagcca acactaactt catttaattg actagaattc ttaatcaaat
     3421 agtcaatact tctttttgct acatctatat tcattctttt atttgtatga actctgttgc
     3481 tgtatgaacc agaataaatg caatagtcac aacgtaaatt acaattttgg gttacttgta
     3541 aaataataga ggatagatta ttttctatat gatattttat atacttagtt tcagggtgca
     3601 ttgttttatt taccctttta ttttttagaa atccataatt aatcaaggta ttaatttcgt
     3661 cttctatttc tatgttccag tcactttctt tttctaaatt atgctcaatt ttatttagat
     3721 aattataaac agcagcattt acctttaaaa tcttatccgt attaacatca taaataaaat
     3781 atgattttcc agctttaaat gtgtgtataa atggtttttg attcattaat taacctccct
     3841 ttaatattta tttgcagtta caaaataaat taaaataaac accctttttg tccataagtt
     3901 catcatgtct tccactttct aacaactttc cattttcaag aactacaata ttgttagtta
     3961 tcttttttgc atgttggata tggtgcgtta taaatattcc aatcttattt tcaaatgttt
     4021 ttttatataa agaaaggagt tcatttcccg cttcagaatc taaagatgaa tctggttcat
     4081 caaaaatgta catatcagca tctttcataa gagttctaga tattgctact ttttgccatt
     4141 cacccataga aagttgcttc ttaccaaacc aggttcctaa aatagtttct aatccatctt
     4201 cctcataaat acttggtttc aatttagttt ctttaagttt acttttaact ttttcttcca
     4261 agttactctt atatacatct cccatggcga cattttcttt taatgtagcc tcatatttaa
     4321 tataatcttg aaacatacaa cttacttttc taatgtattc atctttttca atatccttta
     4381 aattttttcc atttatcaag atgtcacctt cataatcatc ataaaaacct aataatattt
     4441 ttaaaagtgt cgttttacct gaaccatttt ttcctagtat agcaatagga tcatttttat
     4501 ttaaagttaa attaatattt tttaaagaat actctgatga atgattgtat ttatatgaaa
     4561 tatttattaa ctctatttta tttatatccc ctattttttc aaatattttt atatgttcaa
     4621 ttgagtcttc tttacagtta taatctagaa atttaaacag taaatctgta taaaaaagat
     4681 cttgtattac tgaactaaat aacgaaaata ttgattcaac attatccttt atagattgaa
     4741 ctgcttgtat atatgtagtc gcatctccta ttaaaatctt ctttaaaata ccttgaatta
     4801 ttataaatcc tataattcct gcagatacaa ttgaatctaa aactgtacat aatagcgtag
     4861 caataaaatt ctttttagct atattcatat cttgagatat aatatcctct tttaattttt
     4921 tataatgatt aataagttta gaaccaattc taaataccat aatctctttg taagcataac
     4981 cttttgacaa caaataatct atgtaccagg cctttctttc tttccaagtt ctgtttacac
     5041 taacatcata ttgttccttt cctaatttaa tggaatataa tgatttcaat acaggaataa
     5101 caataacaac tattattagc caaagttgaa attgtgataa tacaacaata agagaaagtg
     5161 tagatactga ttctcttatt attgaaataa ggttttcaga tagtatagta atattaaatc
     5221 ctttttgaga ttgagctcta tttatcatgt cataagtatt atcatcctca aaatctttta
     5281 aactcaaagt taaagatttc tctaacatca ttgtttcaat atgtttttca aaatttttat
     5341 taaactttat tctataatat ccataaaaac tttgaaaaat tgaaggaatt atatttatga
     5401 ttaaatacat aattaacaaa actaatattt cttttacact tttagaaaat gtttgcatat
     5461 tattaatgat attttgactt actaacaaag aaatagatgg aacaatcccc tgaaaaattg
     5521 ccataaacat agagataaat aaatatcttt tatcatgtag ataaatcata ttaaaacaat
     5581 ttcttacacc tttaaaaaat tgaaatttac cattttccac aatacacctc catacttaat
     5641 aaaattgaaa taaaata
//
LOCUS       DAFKMX010000211         5648 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFKMX010000211 DAFKMX010000000
VERSION     DAFKMX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.214"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            171..1352
                     /locus_tag="LZB08_003324"
     CDS             171..1352
                     /locus_tag="LZB08_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBM7316789.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1494..2411)
                     /locus_tag="LZB08_003325"
     CDS             complement(1494..2411)
                     /locus_tag="LZB08_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316790.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2664..3458
                     /locus_tag="LZB08_003326"
     CDS             2664..3458
                     /locus_tag="LZB08_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316791.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3459..5519
                     /locus_tag="LZB08_003327"
     CDS             3459..5519
                     /locus_tag="LZB08_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7316792.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2158 a          635 c          923 g         1932 t
ORIGIN      
        1 attatataca aaaatatttt ttaatgtttt tgtgtataat tataacaatt taatttatat
       61 taatgcactt aaatttgaaa tagaatgctt atagtggtaa aattaattat tagaatatta
      121 gaaataataa ataaataata cattgtactt aaaaggggga gaaatttaag atgaacaaaa
      181 aatatattaa agcagggaat ttaattgatg gaaccggaaa tagtattatg caaaataagg
      241 gtataattat tgatggagat actatagttg aaattgaaga tataagagat gatttaaatg
      301 gatatgatgt gtacgattac ttagataaaa ctgttatgcc aggcattatg aattgtcatg
      361 ttcacttgac aatggagcca gttggaaatc caaagatata ttatgataat gtatcagatg
      421 tagagcttgt agtaaatacg ataaaacaac ttgatgctta tttggattca ggagtaacat
      481 atattagaag cttaggttgt cccaaatatg tggatgtaca acttaaaaat cttatagaca
      541 aagggtctat agatggtcct ggtatagtta cttcaggtcc agttatatgt atgacaggag
      601 ggcatggata ttattttggt attgaaagtg atggagtgga tgaatgtaga aaatcagcca
      661 gaacagtttt aaaaaatgga gtagattgtg taaagataat ggcaactgga ggtgtaacaa
      721 ctgatggagt tgagccaggg tcaccacaat taactttgga ggaaatgaag gctgctgtag
      781 atgaagctat aaaagcaggt aaaataactg ccacacatgc acaaggaaga actggtataa
      841 aaaatgcagt gcttgcagga attacttcta tagaacatgg tgtttattta gatgatgaaa
      901 taattgattt gatgcttgaa agaggaactt acttagttcc aactgtggct gcaccatatt
      961 ttattctaaa tgcaggaaaa gaaagtggaa taactgaagc tactcttaga aagtgtgaga
     1021 ttgttgccat tccacacaaa gagtcattcc taaaagcata taaaaaagga attaaaattg
     1081 cagttggttc tgatgcagga acttcttata atgaacatgg aaaaacatat tatgaaatga
     1141 aactaatgtc agattatggt atggataata tggatataat agttgctgct acaaaaacag
     1201 catctgaact tttaagaatt gataaaaatt atggaactct agaaaaagga aagaaagcag
     1261 atgttatagt tgtaaatgga aatccagtag agaatataga cgtacttgca gatgtgttag
     1321 cagtatttaa acttggtaaa aaagttagat aaatagtata aaaataaaaa agagtgtcta
     1381 aagaaatttt taaataatta tagacactct tttttattag taataattaa atcttttata
     1441 ctttagaaaa tagattctat tttatattta gatataactc tttaaaatca ttcttatgca
     1501 atataatcta tagaatctac acatacaaag tatattcttg aatcattaga atttccaagt
     1561 actataacat catttttctt acctagaact tcaacaccag ttagaaccaa actaccagct
     1621 actacagtaa cttgattatt tatatttgac atataaagat tatctaggat acctttatta
     1681 caacagcaat catcacaatc gcagttgcaa ttacattctt tgtaatgttt tttatcaaga
     1741 tagtaaccta aagatgctat gaaatcttca taatctgcag gtgtaatatc aaatgcaaca
     1801 gctttaagat tacacaatga tgctaaatct acgtttgcta atggagtaga tgcagcaagt
     1861 ataaaatcaa ttatagcagc aagtattgca tctaatatag ccccaaggtc tatcgtagga
     1921 ggaataactg catcaataag tgcaattaat tcagctactc ctggtatagt tcctatagta
     1981 ttaattaatc cctcaagagt taaagggact ggaataggat aagatactct accagctata
     2041 tctattaaat cacagttaca agcagaaaat ctttcaaaag taccatcaag tccagataaa
     2101 ttatcttttg gaggagctga aagatctata cctaccaaat tagcacctac tataaagaaa
     2161 tctgagataa aagcaaattg attaaagttt acaaaaggtc ttagagcatc atatcttaga
     2221 agttctaagg cctctctcat acttttttta caacaacatt gttttatttt aggttcttta
     2281 tatttatttg gataattata gtatctatca gctacttgat aattttcata gtatctttca
     2341 tttgtatttg gataatcttc ctcatattct tgtgtaaatc taaagtcctc tctacattta
     2401 ttattttcca ctaaatacac tccttttata ttttaattta aatgtataga taattaagct
     2461 tatctatata gtaatattat atttataatg taaaaaaatg ttacaaaaga tattataaac
     2521 gaataaaaaa taataatttg tcagataaac ttgtaaattt ttatgaataa tccattctaa
     2581 aacaaaaaac tgtgtataaa aaaggtttat atgttatatt tatataaata taacatatga
     2641 acgagaaagg agtggtttga taaatgattg aggtagataa attgtgtaag tcttttacaa
     2701 gggttgtaaa agatgataag aataaaagtt caataagaaa gctaaagaaa gtaaaaacta
     2761 aaaaagaaga attttttgct gtaaataatg tttcatttaa agttatggaa ggtgaaattg
     2821 ttggaatatt aggtccaaat ggtgcaggaa agactacatt acttagaatg ttaggtggca
     2881 tattaacacc aacatcagga agtataaata tttctggata tgattattca atagatagaa
     2941 attcagcaaa aaaagaaata ggttatctct ctggaaatac aaaactatat ggaaggcttt
     3001 ctccgagaga acttctaaca acttttgcaa gtctatatga gatgtcaaaa gaagatatag
     3061 aagaatcaat tgaaaatatt gtaaaaataa tggatatgag tgaatttata gataatagaa
     3121 ttgaaaatct atcaactggt cagactcaaa gaacatctat agctcgttgt ttaatacatt
     3181 ctccaaaagt atatattttt gatgagccaa cactaggtct ggatgtttta agtagtgcat
     3241 ctattataga ttttatgaag aatgaaaaac taaggggaaa aactgttctt tactcaactc
     3301 attatatgga agaagcagaa actttatgtg acaagatttt tatgattcac aatgggaaaa
     3361 taattgcatc aggaacacca cagagtttaa aagaggaatc tggagtaaat aatttgagag
     3421 atgtattcat aaaacttgcc agaaaggagg acatttaaat gagaagtaag attgtaaaat
     3481 acatatttaa aaaagaaatg ctagacatat tgagagataa aaagacttta tttatgatga
     3541 ttgttgtgcc acttctttta tatcctttac ttatgttatt aatgattcaa gtaatgaata
     3601 tgtctgcaag tagtatgaat agtaaggaca ttaatatagc ttttaataat aaacctaata
     3661 aggttttagt atccatgatt gaagatgata atttagaaaa agatactaaa aatacttcaa
     3721 atgataaagg caaaataaga gtgttagatg tagataacta taaaaagtca ttagaagaaa
     3781 ataaaattga tgcttatata aagatggaag aaaaaaaatc atctataaat tatcaaatat
     3841 atataaattc ttctcaagaa aattcttcta atgcactaaa aagaattgaa actgttttgg
     3901 ataaatataa aagatttacc atagaaaaaa ctttatctga aaaaggatta gatgtacaag
     3961 ctactttaga accaataaca tatagcactg tagatgttgc aaagacggaa gaagtggcag
     4021 gatacttttt aggtcagata cttccattta tattaataat aggagtttta ctaggttcaa
     4081 tatatccagc aatagatgtt atggctggag aaaaggaaag aggtacatta gaaacattat
     4141 ttacactgcc aatatcaaac ttagaactgg ttatgggaaa atatatggca gtttctctta
     4201 gtgcaatagt aacagcaatt ttaaacattt tatctatggg actgactatg gcatttattt
     4261 tagttagtgg aggtattagt agccaatttg gctttggaaa ctttaattat ggagacttag
     4321 tcttaccaat tataactact ttaatttgca tatgtctttt ttcaatggta gtttcagcaa
     4381 taagtatgtg tgtatgttca ttagcaaaaa gttttaaaga tgctcaaaac tacataacac
     4441 ctcttatgtt aattattatg ataccatcat atgtttctat gataccaaat atagtattag
     4501 atagagtgac agctgtaata cctgtagtaa atatatcttt acttataaaa agtgtattat
     4561 cttttaaaag cgatttaaat ttaatagcct tagttttagc atcaaatgta gcctttgtta
     4621 tattggcaat catactactg tctaagatgt tcaattctga ggaaatactt tttggtaata
     4681 gtaagagttt ttctttctta gagaaaagaa gtaatataaa aaaagggtct ataccaactg
     4741 taagtgatgg tgttatttta tatgcagtag gattagttct attgatttat gttggttctt
     4801 tagttcaaat taaatttggt atggcaggta ttataatgac acaattcatg ataatttctt
     4861 taccattatt atttgcatac tatattaaag ctgattttaa agaggtattt agcttgacag
     4921 ttcctaaatt aaagcatgtg tttgggagca ttgttctttg gattggtgga tacatactta
     4981 ttatgattat aacacagcta attttattct tatttcctca aaatatggaa gttgctgaaa
     5041 gtttaaataa ggctcttttt atcaaagata gtgttttact gaatttacta gcaatagctt
     5101 tattaccagc tatatgtgaa gaaatgtttt ttaggggatt tatattttct tcatttagta
     5161 aatcaaaaga taaaaataag tctatcaagc tagctataat atgcagtggg gtactatttg
     5221 gtattatgca tatggatttt attagaataa ttccaacatc aatattaggt attatatttg
     5281 catatagtgt gtataaatct ggttcaatat ttgtatcaat gttattgcat tttttaaata
     5341 atagtgtagc agttgtgtta aatcattata caactggaaa tataaccaac ttatataaat
     5401 ttgttgaagt tgatttttcg aacttaaatg tacttaaatt tgccttaatt cttctaataa
     5461 gtcttatatt aattatgtta ggattaagag ttttagatag aaagtcttta agaaattaag
     5521 tataaagtta ttatatagat taaaatatta ataagcacta aagtatatgg atatatcttg
     5581 tatataaaaa tactttagtg ctattttata tattttaatt atatgaatta tttatcgttt
     5641 catttatg
//
LOCUS       DAFKMX010000212         5615 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFKMX010000212 DAFKMX010000000
VERSION     DAFKMX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.215"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     regulatory      31..133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            227..1603
                     /locus_tag="LZB08_003328"
     CDS             227..1603
                     /locus_tag="LZB08_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBM7316793.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1717..2862)
                     /locus_tag="LZB08_003329"
     CDS             complement(1717..2862)
                     /locus_tag="LZB08_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBM7316794.1"
                     /translation="MNTFEQLKISSILIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3115..3822
                     /locus_tag="LZB08_003330"
     CDS             3115..3822
                     /locus_tag="LZB08_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316795.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3869..5464
                     /locus_tag="LZB08_003331"
     CDS             3869..5464
                     /locus_tag="LZB08_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316796.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILGSIL
                     TLILDFMIWRIISTKGVRIFKNL"
BASE COUNT         1902 a          653 c          887 g         2173 t
ORIGIN      
        1 tataaattaa aataatattc gtaaagaaaa attcatgttt tgtactgtat aacatataat
       61 tttgacaata tgggtcataa gtttctaccg gaataccgta aatattctga ctatgtatac
      121 tattatttag ttaattaaat atttaattaa ctgaattatg aaggatacgg ctcagcttat
      181 caactactgt gtcgtttttt tgtaaaattt aataggaggt gttgttatgc aaacaacaaa
      241 cagtagtact atgcagaaac tttttccaat tttgactaat aaaaatgtcg acatgaagaa
      301 ggaaataatt gcaggagtaa ctactttttt aactatggct tatataatag ctgtaaatcc
      361 aaatatttta tctgagacag gcatgcctgc aggtgcactt gttacaggta cttgtttagc
      421 agcagcattt ggatgtattt taatgggtgt agttgctaac cttccttttg ctttagcatc
      481 aggtatgggt cttaatgcat tttttgcata tacagtagtt ttacaaatgg gtgttccttg
      541 ggaagtagct cttacagcag tatttgtaga aggtattatt tttatagtat tatcagtaag
      601 tggagttcgt gaggctgttg taaatgcgat accaaaaaat atgaaattag ctgttacagg
      661 tggtatagga atatttattg cacttatagg tctagttaac agtggaattg ttattggaga
      721 ccaagccact cttatcaaaa tgggtagatt tacaccagca gtaataataa cttgtgtagg
      781 attaataatt attgctgtat tagataaaaa aagagtaaaa ggttctatac tttttggaat
      841 agtagtgagt tcattacttg cttgggcatt tgcttttata aatccagagt atgcacaaaa
      901 attaggtata tatttacctg gtggattatt taaattcgaa agtttagcac caatagcagg
      961 taaaatagat ttaggatatg tactacatcc aacaaatata ggtggttttt tagtagtagt
     1021 ttgtacattt ttatttgttg atttttttga tacagtaggt acattagttg gtgtttgttc
     1081 taaagcaaac atgttagatg aaaaagggaa tgttcctaat gtaggtagag ccttacttac
     1141 agatgcggtt gcaactacag ttggtgcagg acttggagta tctacagtta caacttatgt
     1201 tgagagttca acaggagtta tcgcaggtgg aagaactgga tggacagcaa taacagttgg
     1261 aattttattc ttagctgcaa tgtttttctc tcctgtattt atagcaattc catcatgtgc
     1321 tacagcacca gctttaatat atgttggata tttaatgctt ggtacagtta aggatataga
     1381 gtttgataat ataactgaag gtgttccagc ttttgttact attgcttgta tggcattaac
     1441 atatagtata ggtgatggtt taactttagg tatattaact tatgttttcg taaatatatt
     1501 ttataatata tttggagcta aaaaggcaga agacaaaaaa catgtttctg tagtaatgat
     1561 tgttctggca atattattta taataaaact ttggctacta taataggtat tatttaaaat
     1621 tttaatttaa tataaaataa agggctgtaa tttattatca attgtgatga taaactacag
     1681 ctcttttgta tttgttgtat ttttgtattt attgtattat ttaataatgt ctattagttc
     1741 tccattttgt aaagttttag gtgtaattac tatattgaac tctttttgca aatcttttat
     1801 aatatctaat tctttttttg ttattattga gattgtgtta ccacttcgat ttcctctagc
     1861 agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcca agttaaaaac
     1921 atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa ttaaaatctt
     1981 tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt ccatatttcc
     2041 aaaaattcct attgctttgt aattatgata atttagtttt gatactagga cttctatatt
     2101 tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta atgcctttct
     2161 aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga tgttaggatt
     2221 tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt tatcacaaat
     2281 ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat ctcttaacgt
     2341 tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat tgtccacttc
     2401 atctaagaca atagttttta tattatgtga ttttagtttc ttttgtttta tcaagtctaa
     2461 aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat tttttatttg
     2521 tttttgtata ttgacctccc caattaaagc taaagaagta acggagagtt ctgcattttt
     2581 agccaataat tcaacctggt ttgtaatctg cattactaat tcatgcgtag gtgcaagtat
     2641 aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta aaaggtacgc
     2701 tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt gaattatatt
     2761 tcctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt tgagtccatc
     2821 tattagaatg gaacttattt ttagttgttc aaatgtattc atattttgct ccttatattt
     2881 taataattat agtcaactat tttttattat acattgttta ttcaaatcga actatatatt
     2941 cttttataat tatgtgtaat ttgaaagtaa atatcaatta tatttatacc tttatatttc
     3001 tgataccttt gcattttata gatttctaaa ataaacaaat aaaagttgta attgcttgat
     3061 atttgttata tattatatat aacaaatatc aaaactataa tatggaggga aattatgtta
     3121 aaagtaatca atgtatctaa atcatataaa aataaaaaag tagtaagtag tgtctcattt
     3181 gatgtaaatg atggagagct ttttgggttt ataggacata atggagcagg taaaactact
     3241 actattaaag caattgttgg aatacacgat tttgaagagg gagaagtact aataaattct
     3301 aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa tggcatatat acctgataat
     3361 ccagatttat acgagtcatt aactggtata caatacctta attttatagc tgacatattt
     3421 gaagttgcca aagataaaag agaagagtta ataaaatatt attcaaataa gtttgaactc
     3481 aataaagcat taggagattt aatatcttca tattcgcatg gtatgaaaca aaaattagct
     3541 ataatttcgg ctttgataca cagcccaaaa atcttaattc ttgatgaacc ttttgtaggt
     3601 cttgacccaa aagcttcctt tatattgaag gaaattatga gggagttttg tgacaaaggt
     3661 ggttgtatat ttttctcaac acacgtttta gaagttgctg aaaaaatttg tgataagatt
     3721 gccataataa aagatggaaa tataattgct tatggaacta cagaagaagt taagggaagt
     3781 aattcacttg aaagtatctt tatggaatta atagagaaat aaaatgtttt ttatacaata
     3841 tagtgtctaa agtgaggaat gataaaatat gagcaatcta agtatattaa taaaaactaa
     3901 tataataaat gaatttaaat taaatgcgtt aaaaaaagct gatggtagag aaaaacaaaa
     3961 aataatggga atgatgcttt cagttgttat aatagcgggt ctattaattt tttatgtaac
     4021 gagcttaagt ctctctttag ttgatatctt aaagcaaata aatcaactgg aaatgctact
     4081 gatatttggt tttggattat caacattaac aactattgtt acatcattat ataaaacgtc
     4141 atcatatcta tttcaagcaa aagatttaga ccttttgact agtttaccta taaaagaatc
     4201 tactgtacta gcaagtaaga tattaatgtt ggttggagca aactatttat tttcttcaat
     4261 atgtatgatt atacctgcag tggtttactt tttaaattct gatgtaagta taacttattt
     4321 cccttatttg atattgctat ttttagcttt accattatta ccgatagttg cttcatcaat
     4381 aatttttcta tttatagggc agatatcatc aagaataaaa tacaaaaatt tagtattaat
     4441 cataggtagc attatattga tgttgcttta taccctactt atgtcaagac ttaattctat
     4501 gtcagttgaa atgctaaaga atagtgtgtc tatttcagag acgattagaa aagtatatcc
     4561 tcctatatat tattttgttg atgctcttaa aacaggaagt atagtttcat ttttagcatt
     4621 tatagccatc tcaataatac cattttttat gtttgtaact ctgtttgcaa aaaaatttaa
     4681 agatataaat gctaagatga gtgaaggata taagtcaaaa aattatgaat ttaagggtca
     4741 aaaaacttca agaccttcaa aagctttatt aaaaaaagaa tttaagagat atacttcttc
     4801 gtttatatac tttttaaatt catctgcagg tttgttattt ctaatagtag gtacaatttt
     4861 aatattatta tttggagcag ataaagtggg tgagttactg aaattgaata tagattttaa
     4921 tcttataaaa atacaattat tgggagtatt cttctttata atagtaatga attgtacaac
     4981 atactgttca atttctcttg aaggaaaaaa cttatggata ctgaaatctt ctccaataga
     5041 tgaaagagaa atattctggt caaaaatatt gatgaatttt ttattgaatg caccactatc
     5101 tattttatgt ctaatattag tatctataag attagacttt gaaattcagt ttattattgt
     5161 atcaatctgt ttgattgtaa gtttttcaat gtttgtagca ttgatgggat tattatcaaa
     5221 tttattatac ccaaaccttg attggaaaaa tgaggtggct gtagtaaaaa gaagtgcaag
     5281 tatgatagtt acaatgttag tatcattagc ttatgtaatt atacttggag gtatatatgt
     5341 attacttgga atttcgtttt taaatatata tattattttg ggaagtatac ttactttgat
     5401 attagatttt atgatatgga gaattatttc tactaaagga gtaagaatat ttaaaaattt
     5461 ataggtgtat ttatgtcaaa taaagactta tcttttttag tcttgcaaat aataggttaa
     5521 gtagtatact atgtatagtg atatgaaaaa atttaggaga aaataaatga gaaaattatc
     5581 caaaagagaa aaagatttta ttcaatatgg atttt
//
LOCUS       DAFKMX010000213         5581 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFKMX010000213 DAFKMX010000000
VERSION     DAFKMX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.216"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(137..820)
                     /locus_tag="LZB08_003332"
     CDS             complement(137..820)
                     /locus_tag="LZB08_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBM7316797.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1101..1691)
                     /gene="zapA"
                     /locus_tag="LZB08_003333"
     CDS             complement(1101..1691)
                     /gene="zapA"
                     /locus_tag="LZB08_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBM7316798.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1716..4109)
                     /locus_tag="LZB08_003334"
     CDS             complement(1716..4109)
                     /locus_tag="LZB08_003334"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBM7316799.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVRYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSIIYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4126..5145)
                     /gene="pheS"
                     /locus_tag="LZB08_003335"
     CDS             complement(4126..5145)
                     /gene="pheS"
                     /locus_tag="LZB08_003335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBM7316800.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5195..5429)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1706 a         1080 c          598 g         2197 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tacaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataatagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aataaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttaca cattatttgt atttatatag catttatatt
      961 tatttatatt taaacattat ctatatttat atagcattta tatttattta catttaaaca
     1021 ttatctatat ttatatagca tttatattta tacattaaaa gaagctgtct caaaaaacaa
     1081 gagacagcaa atcttttagt ttattccaca ttttttgcat cattatttaa ccctgtacat
     1141 tttaactgta aatcataagc tttgttttga aactcagaag ctaagttttc agctatagta
     1201 gctctttcat ttgcttcttc aagttgagta cttaactcct caatcttgtt tttataagca
     1261 tctaactctg ctctagaacc ttctgtcgta ttactaagtt cgaatatttc ttgcttttta
     1321 ttttctataa tctcaattaa ctctttcatc ttagtttcag cttcacgatt ttctgcttct
     1381 ttttctgcta tcgtaagctt taaagatttg atttctaatt gaagctcttc attagatgct
     1441 ccaaccttct tactcaattc ttcattcgct ttaatcaact tctcattctc atctgaacat
     1501 tcaaataaaa ggtctgatat atttaatgca gttaatatcg cagcaactga taagctcaat
     1561 ttcgggtttt gacgtgttat tttatccatt tctttgtcta caaaatcagc aatactaatc
     1621 ataaacttct ctgatttgtc tccaaccatt ggatattcag cgccatggat tttaaccatt
     1681 actttgttca tcctaacctc ccccgacatt agttgttaat ttgatcttaa gttagcattt
     1741 aatttttcgc ttaattcact taatatctta tcatgcacct tagctacatc ttcatctgtt
     1801 agagttttat ctttacttct ataaattatt gaatatgcaa ttgacttgta tccagcttct
     1861 atttgagaac ctttgtaaac atcaaataac ttgtagcttt ctactaatcc ttgaccattt
     1921 tcctttatta tatcttctat ttgttttaca aatacatcat ctttaacaat aagagctata
     1981 tctcttgatg tagatggata ttttggtaat ggcacataat tctttgttct atcagaatta
     2041 tcaaatacaa agtcaatatc tatttcagcg acataaactc tttgacctag attgtagttt
     2101 tctataacat ctggatgcaa ttcacctaat gtacctacat atttattatt atacactatt
     2161 ttagcacatc taccagggtg gaaggtagta ttattatctt gaggttcaat ttcataacct
     2221 ttaaatccga catttacaag tatagtttct atagttcctt ttagtgcaaa gaagtcaaca
     2281 tctttaccat acatacctac acacattcta ttttcttcct taggtaattc tgaatcttgt
     2341 gggatgaata tatgtccaca ctcaaatgca gatacttctt ctattttatg agatacatta
     2401 gttgataaaa catctaacat atttggtatt aaagtagttc tcattacaga agtttcttct
     2461 cctaatgggt taagtaattt aacaaaattt ctctttgcat taccttctgg tactcttatc
     2521 ttatctactc ccttagggct tacaaatgaa tatgttaata tttcatttaa tcctacagca
     2581 gttgaattat cttttaaagc atccatgaat ttttgttttg aagttttaat tcctgctgtc
     2641 gtatttcctt ctaattgtac agaaggtata ttctcaaatc cataaattct agctatttct
     2701 tcccatacat cagcttcttg ttccatatct gttctaaagc ttggtacatc tatttctaat
     2761 ttatcattag ctaccaaatt acatttaaac tctaatgatt ctaaaattcc tacaaactgc
     2821 tccattggta catctacacc taataggtgg ttaattcttt gaggatttac taccaatttt
     2881 ttaggttctg gtttatttgg atatacatct acaacacctt ttaaaacttt tcctgctcct
     2941 aacatttcaa caagttgtgc agctctattt gctgctatct ctgcaagatt agggtctaag
     3001 tctttttcat ttcttgaaga tgcttctgac ctaataccaa cttttttagc tgtcattctt
     3061 atgttttctg gtttgaaatt ggcactttca aaaagtacag tcttcgtatt agaagttatt
     3121 tctgagttag caccacccat tacaccagct aaaccaagtg atttttctcc atttgttata
     3181 actagcatat ctttgtctaa tgttctctca acatcatcta atgttacaag tttctctcca
     3241 tctttagcat ttcttactac tattcttcca gtctctactt gatttatatc aaaagcatga
     3301 agtggttgac caagctctaa cattacgaag tttgttatat cgactatgtt acttataggt
     3361 cttactcctg cttctgtaag tcttctttgc atccaatatg gagaaggttc tatttttaca
     3421 tctgtaacca ttctagcaac atatcttcta cataaatctg gattatctat ctcaactttg
     3481 aaatctatct cttcgtcact ttcttttact tcgatttcag gatatcttac ttttgttcct
     3541 atagttgcag ctgcttctct agctataccc atcatgcatt tacaatcagg tctgtttgaa
     3601 gttaattcaa aatctattaa agcatctttt aatcctaaaa catcttttat atcttttcct
     3661 aattcataag aatcttcgtg gtctaaaata tatataccac ctcttttata ctcctcaatg
     3721 taatgttcat ctatacctag ttcagctgct gaacacatca taccatctga taattcacct
     3781 ctgaaatcag tctgtttgat ttcaactcct ccaggtaact tagaaccatt tacagctact
     3841 ggaatataat ctccttctga tatatttgta gctcctgtaa ctatttgaac aactttatct
     3901 ccaatatcta ctttagttac aaccaactta tcagcatttg gatgttgttt tatttctaaa
     3961 atctttccaa ccaatatatt ttctatttct tcaccataat aatctattgt ttcaacttta
     4021 gttcctgtca ttgtcatttt atcagcgaac tcttttacat ccatgtctat atcaacatag
     4081 tctctaagcc attttaaaga tactaacatc tacataccct cctaattaaa attgatttaa
     4141 gaatctcata tcattttcga ataataatct aatatcatct atttcgtatt taagcattgc
     4201 aagtctttca accccaactc caaatgcaaa tccactataa acttctgggt ctattccaca
     4261 atttctaagc acatttggat gaaccatacc tgaacctaat atttctatcc aaccttcata
     4321 tttacacatt gggcaacctt taccaccaca tttgaaacaa gtaacatcaa cctctgcact
     4381 tggttctgta aatgggaagt tgtgaggtct aaacttagtt ttgatatcag aaccaaacaa
     4441 tttttcaacg aagatatcca tagttccttt aaattctgcc atagtaacat cttttccaac
     4501 aacaagccct tctatctgat ggaacattgg tgagtgtgta gcatctggcg agtctgacct
     4561 aaaacatcta cctggtgcaa ttacttttat tggtaactct tgacttctca tagttcttac
     4621 ttgaactgga gatgtttgag ttctaagtaa tactccatca ttgatataga atgtatcgct
     4681 catatctctt gatggatggt ctttaggagc gtttaatgcg tcaaagttgt tttcaacagt
     4741 ctcaacttct ggcccttctg ctatagagaa tcccattccg ataaatatat ctgttacttc
     4801 atctataatt tgagttattg gatgcttctt cccaaccttt aaaacttttc ctggttgagt
     4861 aacatctatc acttcctctt ttaattttct ttctttttca atagcattta tttcttcttt
     4921 tttagaattt atgctaagtt caatgttttc tcttacctca ttggcaacct taccaactac
     4981 tggtctttct tcagcagata atttacccat ttctttaagt atggcagtta tctcaccttt
     5041 ttttcctaag tacttaactc ttaaactttc tacactttct atgctttgtg cttcttttat
     5101 ttcagccaaa gctgcttcac gtaaagcaag taatttttct tgcacacata tcaccctttc
     5161 gtttttttgt ataaaaaaag cccctcaatc cctgtctagg gacgaaaggc tatagtttcg
     5221 cggtaccacc ctagtagttt aacaatttaa aaatcatcaa accacctcta acaaacttaa
     5281 cgcgtttttg acgtcaaagc ctactgtaaa atttcagctc tgttactcca gagggaactt
     5341 ctcatgacct cttagaaaac actcccagcc taggtgttta tctctttact aagtttaatc
     5401 aaaatacttt cctctttcat tgcatttaca atattatatt gtttatttta gtattatatc
     5461 atttccattc aaattatgtg actattttta atatatatcc aatacataat taatttaatt
     5521 attttttata taatatatat ctaatgcata attaactcaa ttatttttta tataatatac
     5581 a
//
LOCUS       DAFKMX010000214         5524 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFKMX010000214 DAFKMX010000000
VERSION     DAFKMX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.217"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            153..1853
                     /locus_tag="LZB08_003336"
     CDS             153..1853
                     /locus_tag="LZB08_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316801.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2023..2640
                     /locus_tag="LZB08_003337"
     CDS             2023..2640
                     /locus_tag="LZB08_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316802.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3001..4194
                     /locus_tag="LZB08_003338"
     CDS             3001..4194
                     /locus_tag="LZB08_003338"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBM7316803.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4212..5504
                     /gene="lysA"
                     /locus_tag="LZB08_003339"
     CDS             4212..5504
                     /gene="lysA"
                     /locus_tag="LZB08_003339"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBM7316804.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
BASE COUNT         2191 a          575 c         1047 g         1711 t
ORIGIN      
        1 atacatacac gtataatgtt aaatatagtt gaataagcag aactataaat atataaagat
       61 atcttaatat ataataaata tgataaatat ttattttttg taaatatatg gatataatag
      121 taataataac attttttaga gaggagtatt aaatgaaacg atatcttaaa acatatggag
      181 ttgcattaat aattgtaata atttatgcat ttattaaatt acctgtacta agattagatt
      241 ttacatcttt attttcagta ggaataattt tctttgttgt agcaggaata ctagatatga
      301 tgttagatag aaatgaaaga gcatctaaaa tggctaaata taatttttca atagcaatag
      361 ctttgcttgt gtatattgta gtagttccct ttataacttc tacacctgta cttcatgcaa
      421 aatcatatag agaactattg ggaaaagtaa ctgaaagtaa atttactgat gacattagcc
      481 ctgtcagtgt aaatgacatt cgtcttgtag atgaagatat ggcaatgaaa ttaggtgaca
      541 aaaagcttgg agaagttcct gcaataggga gtgtatccaa attaggaaaa tttcatatac
      601 aaaatgtaga tggagagttg tactgggtag cgcctcttgt tcacagagac ataattaaat
      661 ggataacatc tctaagtggt acaagtggtt atgtaaaagt atcagctagt aatcctcaag
      721 acgtccaatt agttcaagaa atagatggaa agccaataaa gatagtttac caaccagaag
      781 cctacttaca tcaggattta caaagacatt tatacataca tggtattgta aatgttggta
      841 tgacagattt cactcttgaa ataaatgatg aaggaagacc ttattgggtt gtaagtttat
      901 atgaacataa aattggctat ggaggagcaa atgcaacagg aatagccact gttgatgcag
      961 aaactggaaa aataaatgtt tatgatgtta aaaacacgcc taaatgggtg gatagaatac
     1021 aaccacaaag ctttgtaaca gaccaaatta aagactgggg tgtttatgtt aatggatttt
     1081 taaactctgt tatatcagaa aagggagttt tagtaccaac agaaggtaca tcactagttt
     1141 atggcaatga taacaggtct tattggtata caggtataac atcttcaggt ggagatgaat
     1201 ctacaattgg atttatgtta gttgattcta gaacaaaaga agctagatta tataaacaac
     1261 caggagctac tgaaacggct gcaatgaaat cagctgaagg taatgttcaa gaaaaaaatt
     1321 atgaagctac gttccctgta atgtataata tattgggtca gccaacatat gtatcatcat
     1381 taaaagataa ggctggtctt gttaagagag ttgcatttgt atctgttgaa gattataatg
     1441 tacttggaat aggtgaagat aagaatgaag cattaagaaa ctataaggat gcattagaat
     1501 ctaagggtaa tgatttaaaa cttgataatg atttaaaaga tgaggtatta gaaggcacag
     1561 tgacaagaat aagcccagat gtgcgaggtg gtaacactaa ttattatgta actttagatt
     1621 caaataaaga tataatattt agggctactt caaaagtatc tagcgaattg ccattgactc
     1681 aggttggtga taaagtcaaa atatcgtatt caaaagaaga atctggtgta atagaaatgt
     1741 cagaatttga taacctaaat attgcaaact ttgaagataa aaaagcaaaa gaaaaaacag
     1801 gaactgatga aaatacagat tccaaagatt ctgaaaatca aggtaatgta taatttaaat
     1861 aaaaagattg ataatagtat ttaacatagt gatagctaat ttttatagaa tttaattcta
     1921 taaaaattag ctattttatt ttaattcttt gtaaatttaa tgtcataaaa ttaatataat
     1981 attagtacaa ctttgcaaat aatacttcaa ggagaatgta ctatgacgaa tatggaaaaa
     2041 gagatattac ttttaattag cagggttaga tgtgttacag agtctcaatt tagaaaaatt
     2101 tatggagacc aaaaaagata caataaaaaa aattttaaaa aaactttgag aaaaatgtgt
     2161 agtgaatata cactaaaaaa atatccttgt gatgtggatt attataatta taaagacctc
     2221 tcttatgtat actatttaaa cggtagtaaa ctttttaagg gagatgattt aataaaggct
     2281 ctagtaggat cagaattagt tgttagaatc aatactgcag gttgtgatgt aaaaagattt
     2341 taccgaaatg ttagtgtaga tgatgtgaaa tacgatttat ttattgaata tacagaccaa
     2401 tttggtaata taaagcaagt tttagttgat gttgacttaa ataatgaaat agacatttct
     2461 aaatacgaaa atctttctaa aaaaatagat aaatctacaa ttccattttt taaagtacca
     2521 aacgttttgg tagttacacc agataatcta tccgaattaa atgatttaga gagatatgat
     2581 aatgagtata gcataaattt tatagattta ggattaaata aactatttaa ttgtatataa
     2641 gctaacttta gaaaagaaaa ctatcgattt gccacatttt gtaaaataat tgttgcaata
     2701 ttatatagaa tatgataaaa ctatattaat aaattaatta aaaataatga gcctagggta
     2761 gaggtagctg tattcaatag tattcaatag gagctggtaa gcgttgataa ttgatgaaag
     2821 ggtatatagc cgaagaaatt ttgttgacgg acaaattttc tggacttatg cagaatatgc
     2881 ataaggctgt cacttttaaa gtggtgagct acaaggttac ttggtctatg aaatttacac
     2941 gtccaaagta tacctttggg cgtttttatt tatcaaaatt tagcttgagg gggagaaagc
     3001 atgagtataa ttgttcaaaa atatggtgga agttcagtag cagatactga aaaaataaaa
     3061 tcaatagcag aaaatataat agaacgaaga aaagaaaatc cacaaatggt aatagttgtg
     3121 tctgccatgg gaaaatccac tgatgaatat ataactttag caaaggaatt gagtaatgaa
     3181 cctagtaaga gggaattaga tgctttaatg tctactggtg aaatgatttc agcatcttta
     3241 ttatcaatag cattaaatgc acttggatgt aaagctataa gttataatgc ttatcagtta
     3301 aatataaaaa cgagtggtct tcatggaaag tctcaaattg atgatataaa cgtaaacaga
     3361 attaaaaata gtttagatga aggaaatgtc gtgatagtaa ctggatttca gggaatcaac
     3421 gaagatggtg atgtaaccac tcttggaaga ggtggttcag atacatctgc tgtagctctt
     3481 gcagttaaat taaatggaaa atgtgagata tatacagatg ttgatggaat ttattttaca
     3541 gacccaagaa aatattcaaa ggctagtaaa cttgatgaga ttgaatatga agaaatgctt
     3601 gagttagcga gtttaggagc acaagtaatg cattcaagaa gtatagagct tgcacaaaaa
     3661 tatggtgttg aaatctatgt cggacgtaca tgtggtacag aaaaaggaac atatataaga
     3721 gggggaaaag acatgaaatt agaggataaa gtaataacag gattagctac tagtgatgat
     3781 gacagttcta taacaataaa agattttaaa gctgaaaata tatctagctt atttgaagat
     3841 atagcaacta taggtataag tgtagatatg ataagccaaa ctgcacctat tttagataag
     3901 ataagtgtat catttacagt tccaaaagaa gagttgggag aatgtaaaaa gatagtatct
     3961 aaatatacag atgaagaaca tgtagtgatt gataataata tcactaaatt ttctttagta
     4021 ggtcttggta tgaaaaatac ttctggagtt gctgctaaag tatttaagat attcaatgaa
     4081 aatggtataa tgataaaact tataactaca tcagagattc gtataacttg tgctataaat
     4141 tctgatgata agcaagtagc tatagaaaaa atagcagaag tatttaatat ataaaatttg
     4201 gggaggctat tatgaaactt catggaacta tgactgtaaa taatgatgaa ttatatattg
     4261 gaggagtgag ttgtttagaa cttagtaaaa aatatcaaac tccactttat gtatttgatg
     4321 aagctttagt tagacaaaat tgtagagaat atatagaata ttttaaggct agagagggaa
     4381 aaaataaggt tgcttatgca gggaaagctt ttttacctct ttacatgtgt aatcttataa
     4441 atgaagaaca aatgtgttta gatgtagttt caggaggcga gttatataca gcttataaat
     4501 caaattttcc tatggaaaaa gttttctttc atggaaacaa taagactaaa gaagaaataa
     4561 acatgggatt agatttaggt gtaggtaaat ttgtagtgga taatttttat gaattagatt
     4621 tgcttgaagc attatgtgaa gataaaggta aaactcaaga gatatacttt agaataactc
     4681 ctggtataga agctcataca catgattata taaaaactgg acagatagat tctaaatttg
     4741 gatttgcttt agtaaatgga gatttattta gagcaataga aaaattaagt gaatataaaa
     4801 atattaaact tgtaggtctt cacgctcata taggttcaca aatatttgat gtagaaccat
     4861 acatagatac agtggatata atgatgaact tggtaaaaga aataaaagaa aaattcaaca
     4921 tacaactaac agaaattgac ctaggtggag gagttggcgt ttattatagc gaaggagata
     4981 aacctaaaac tataaaagag ttttgtgaaa ccataatcac taaagcagaa acttcatgta
     5041 aagagcttgg aatagaagta cctacattgg taatagaacc aggaagatca atagttgcaa
     5101 atgcaggaag tacattgtat acagtaggtt ctataaaaga tataaaagat gttagggttt
     5161 atgtaagtgt agatggaggt atgacagata atgtaagacc ttcgttgtac caagctagtt
     5221 atgagtgtag tattgtgaat aaaattaatc atgaggctat gaatcatgta acaatagctg
     5281 gaaaatgttg tgagagtgga gatatattaa ttggagatat aaacataatg gatattaaaa
     5341 gtggagatat acttataaca acatcaactg gtgcatatgg atattcaatg tcttcaaatt
     5401 ataataaaat accaagagca gcagtagtat cagtatttga tggaaatgat aagataattt
     5461 gtaagagaca aagctatgag gacttattag ctttagagat ataaaataga gaagtatata
     5521 ataa
//
LOCUS       DAFKMX010000215         5460 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFKMX010000215 DAFKMX010000000
VERSION     DAFKMX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.219"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            531..1838
                     /locus_tag="LZB08_003340"
     CDS             531..1838
                     /locus_tag="LZB08_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316805.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1924..2186
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2312..3115
                     /locus_tag="LZB08_003341"
     CDS             2312..3115
                     /locus_tag="LZB08_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7316806.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3153..3764
                     /locus_tag="LZB08_003342"
     CDS             3153..3764
                     /locus_tag="LZB08_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBM7316807.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3796..4467
                     /locus_tag="LZB08_003343"
     CDS             3796..4467
                     /locus_tag="LZB08_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBM7316808.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4766..5128
                     /locus_tag="LZB08_003344"
     CDS             4766..5128
                     /locus_tag="LZB08_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBM7316809.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2208 a          484 c          942 g         1826 t
ORIGIN      
        1 aaaatatatt aacataaagg taaaattttg tatactaaaa gggaatattt acagtttaat
       61 ggcagtttta aaataataaa ttaaagctgt catttttatt taaaaatttt tagaaaaagt
      121 acaatagtgt taatgtgtga attagataag gtgtatataa tagtttagat gatatgatat
      181 aaaatatgtt gtaaattaaa tgttttcttt gacaatatat acacaaggat gataatatct
      241 atatgaataa aagatagtgt gtaatttgat aatatgtaat tataacatta tgatataatt
      301 acaataaagt atgtaataat ctgtagaaat ttcaagctag ttatctaagt tttcggataa
      361 ctaaatctaa acggaataca taatgtatta aataaatgat gatactaaaa tgatactatt
      421 tttatagaaa tttattgagg tatataatta atgaaaataa tatattaaat tatataaatt
      481 aaattatata atatattgag ttttacataa aagcaaaggt gggaataaaa atgaaagaaa
      541 ttgctgatgc tataacatca gagtatttaa gctatggtat caatgaagat attagtatct
      601 atgaaggtag attttgcata tatttagata aaaaatatag atgtaatggg aaaatttatt
      661 ataaaatgac acctccaata tctataagtt ttaaagcaga cataggttgt gttgaagaga
      721 tagacaatga agatgacaat ttagctttag attatgataa tgctattctt gaagttcatg
      781 gatataaaat tatttctata acaataaata ctttaagtga atttagtgta gaaggttata
      841 tcaatgatga ctgcataaaa tctaagaact cctatgttga atatgtagat tttaatataa
      901 taaacttaga taaaatacct ggaaaactca ttaaatacaa tgataaggta tacgcaggta
      961 gaattgaatt tgatataaat gattatgttg taactataga caagcgatat gattatagaa
     1021 aagaattaaa atcagaatta aagtctaaat ctggagcaat aataactcat ataggcagga
     1081 ttagaagaaa agatggtaga atttttagaa caaataatac aataaatctt ttagatagga
     1141 tatcaacagc tttgagtttt atgtgtggaa gatatgtagg gttttgtttg gccaaagggt
     1201 atagaagtgg taatgaagtt tatagaatat ggaatgaaaa tcaaatatct ccttttagat
     1261 atgtaccaac atggtcagat acactttcaa attatcataa tatggaaaaa tatataagcc
     1321 ttatgtgtaa aaagctagaa gatttttatt atggttctgc tataaagagt gttgttgatt
     1381 ggtacataga atctcttgga agtgctacca tggaaaacaa tataatatct gtacaaattg
     1441 cacttgaaac tttgtcatat gtaattctag tggaacaaaa taagatactt acagatgaag
     1501 catttgactg taatttggca tctaaaaata taagactctt acttgataca tgtaagatac
     1561 catatggtaa acatgaatta aatatatttg ataatataat aaaaaataaa tttgatgatg
     1621 gagtggattt agttatttat ttaagaaata gcatagttca tccaagtaga aagactcata
     1681 gagctgtgtt agaggttgaa gatatttgga atataatttc aataggaacg agatatattg
     1741 aacttgtttt actctttatt ttaggttata gaggagaata ttcaaacaga ttagtagaaa
     1801 gatgttatgg tgaagtggaa gttgtacctt ggaactagat ttttatattt attttaaaat
     1861 atatattgac atatattgcc ttatgctata aaatcaatat cataataata attcagaata
     1921 acattaaatg ctaagaaaag aaatagtaaa agtagttaat cttaaacaga gagtttccga
     1981 ttggtgagag gagcaataga ttttactttg aacacatctt tgagcaggac tctgaaagag
     2041 tagaaatact ttattaggag atttccggaa tccttgcacc cgttatagtg ctagagtata
     2101 ataattttat tgtacttaaa gaggttagtg tcgtgaggca ttaatgaaca gaggtggaac
     2161 cacgagagtt aactcccgtc ctctagcaga agctagaggc gggagttttt taatataatt
     2221 ttttaagcgc gcagagaatt atattgcaat agatacactt tttaaaaaat aatcaaaata
     2281 ttttaaaaat taaaagacga gggagaatat tatgaaaaat atattaaaaa aagtaggaat
     2341 atttacaatt atgttaggtt tattaggtgg agttgtaggt tgttcaaaac cagataatga
     2401 aaaagataag gatgcttcaa aggaatctaa aaaagaagta gttgtagggt ttgataatac
     2461 gtttgtacca atgggatttt tagatgaaaa gggagataca gtaggctttg acgtagactt
     2521 agctaaagaa acatttaaaa gacttggaat ggaagttaag tttcagccta tagattggtc
     2581 aatgaaggaa actgaattaa atgattcaaa aactgtagat gtattatgga atggatattc
     2641 tataacagac gaaagaaaga aaatagtatc atatacagaa ccatatttac aaaataaaca
     2701 gataatagtt actctaagtg actcaaaaat aaattcaaaa gctgatttaa aagataaaga
     2761 agttggaaca caacaaggtt caacagcact tgatgcagtg gaaaaggata aagattttat
     2821 gaatagctta aaaggtggag caccagttct ttatgatact tatgataaag cacttagaga
     2881 tttagagata ggtagaacta gtgcagttgt tggtgatgaa gttttaatta gatactatat
     2941 gggacaaaag ggtgaagaca aatacaaggt attaaaagac gattttggat tagaagatta
     3001 tgttgtagca acttctaaag aaaatccaga actttgtgag aaaataaatg aaacattaaa
     3061 agaaatgaaa aaagatggaa cttttgacaa gatttatgat aagtggttta aatagaagat
     3121 aagttagctg ttaagaaggg ggcactaaag gcatgttaca aggtttagag atagttatag
     3181 caatgttttg tataacattg atagtatcaa ttccattggg gattggagtt gcatttttaa
     3241 gattatcaaa gaataaatta gtaagtggta ttacacaatg ttatatattg ataatgagag
     3301 gtacaccttt acttttgcaa atgatagtca tattttacgg attacctctt ttaggaattg
     3361 tatttgacag atttacagct ggagtagtag ctttcttctt aaattatgca gcatattttg
     3421 cagaaatatt tagaggtgga atacagtcaa tcgatagagg acaatatgaa gcttcaaaag
     3481 tactaggatt tgataagttt actatgtata agagagtaat ctttccacaa gtttttaaaa
     3541 gaatactagc acctatttca aatgaagtaa tcacattagt aaaagataca tctttagtat
     3601 atatattagg attaaatgat attttaagaa tatctcaaat agcaatgaac agggaagcaa
     3661 gtctgttacc attatttgag gcaggagcga tatatcttat atttgtagct atacttacta
     3721 agggatttga attacttgaa aaaaaatatt cttattatag ataaagttac aatattatag
     3781 gaggaggttt tttatatgtt gaaaatcaaa aacttaaata aatcatttaa aaaaaataga
     3841 gtacttaaag atatttcttt tgagttagaa gaaggtcaga ttggagtttt acttggtaag
     3901 tctggtgcag gaaagaccac aatattaagg tgtataaatg gtcttgaaga atttgatagt
     3961 ggagagatta taatagataa tgaggtaata aaaaataaga gagatatggc aaaaatacga
     4021 ggaaaaatag gtatggtttt tcaaaatttc aacttatttc ctcatatgac agtccttgaa
     4081 aatataattg aatccccagt taatgttttt aaagttccta gaaaagaggc tgaagagagg
     4141 gctagagaac ttttaagatt agttgattta gaagataaat taaattctta tccatttgaa
     4201 ttgtcaggag gacagcaaca gagagttgct atagctaggt catgtgcatt gatgccaaaa
     4261 gtcctttgtt ttgatgaacc tacttctgca cttgatattg atactattca aagagtagtc
     4321 aatataatga acagacttaa agacaaaggt atgacaatat taataattac acatgatgtt
     4381 gtcttctcaa ataatgttgc agataaaatt ataagtataa aagatggtat agtagaaaat
     4441 gttcaaataa aagaaaaaat tgtttagagt tttataaata taaactaggg tagaatttta
     4501 taatataaat aaagggcaag taaaatcaaa tatactatgc ccttttgtta tctcaaaatt
     4561 taatcttata ttttatgttt aagttacatt ataatttata ataaaaataa aaggactatt
     4621 taaatatata atgtgaataa atgtttaata atttgaaata tgataatttt ttatgttgaa
     4681 agcttataat gattatgata taaattggtt atagtataca atttatgggt ataacaataa
     4741 aaaattagaa aggaattttt ataaaatgat taaattatat ggatatacaa aatgctctac
     4801 tgttaaaaaa gctaaaaatt ggcttaagga aaataattta gaatttgaag atatagatat
     4861 ggttcaaaac cctcctagta aagaggagtt aaaatcaatt tataagacta gtggatatga
     4921 tataaagaaa ttttttaata caagtggtat gaaatataga gaattgggtc ttaaagatat
     4981 tgtaaaaact gaatctgatg ataaactttt agaaatattg gtaagtgatg gtatgcttat
     5041 taaaagacca ttacttttgg atggtaaaaa tgtattattg ggctttaaag aagatgtatg
     5101 gaaaagtact ttgctaaaag aagattagga agagataggt gtgttgatta aaattattaa
     5161 ttaatacacc tataaattta tgtattaaat ctaattttta taaatattaa ttagcaaatt
     5221 caaatcaaaa aaattcatat caacaaacat attacttctt attacaaatt atatcatatg
     5281 ttttatatta aaatattata atattacaac gaattgttac aaaattactt attattaacc
     5341 atgaacattt gttttgtgct atcatattta tataagggaa ttgtcataaa aaatatcttg
     5401 ttcatacagt tttatataga aaaaagaaat gaatcttttc tttatataag tatattttgt
//
LOCUS       DAFKMX010000216         5445 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFKMX010000216 DAFKMX010000000
VERSION     DAFKMX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.220"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            257..1021
                     /locus_tag="LZB08_003345"
     CDS             257..1021
                     /locus_tag="LZB08_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBM7316810.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(1302..2417)
                     /locus_tag="LZB08_003346"
     CDS             complement(1302..2417)
                     /locus_tag="LZB08_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBM7316811.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="LZB08_003347"
     CDS             complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="LZB08_003347"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBM7316812.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4277..5374
                     /locus_tag="LZB08_003348"
     CDS             4277..5374
                     /locus_tag="LZB08_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBM7316813.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         1841 a          923 c          697 g         1984 t
ORIGIN      
        1 atctctatac taataaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
       61 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
      121 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
      181 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
      241 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
      301 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
      361 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
      421 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
      481 ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
      541 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
      601 gaatctaact gtaataacca actcaatttt agttgcaaac gaacttatga atactaatat
      661 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
      721 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggag ctggaggtat
      781 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
      841 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
      901 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
      961 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
     1021 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
     1081 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
     1141 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
     1201 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
     1261 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
     1321 attttaatac tttgttcttc ttccaaagtt aaatcttttg tcataagtat agcaagtgca
     1381 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
     1441 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
     1501 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
     1561 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
     1621 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
     1681 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
     1741 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tattgttatc
     1801 aatatagata taaattttcc aaaaactaat gacccattta tcataagcgt agaattaaat
     1861 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
     1921 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaagg tattgttatc
     1981 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
     2041 cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca
     2101 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
     2161 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
     2221 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
     2281 aaagccaatg gacccattga attaaaccct tcttcaaact gctctccaag tccaaattta
     2341 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
     2401 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
     2461 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttgatt
     2521 ccatccaagt aatatttctt ctcttttttg attagtcata tttggaatat attttttgct
     2581 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
     2641 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
     2701 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
     2761 caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg
     2821 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
     2881 cccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
     2941 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
     3001 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
     3061 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
     3121 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
     3181 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
     3241 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
     3301 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtattcctat
     3361 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
     3421 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
     3481 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
     3541 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
     3601 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
     3661 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
     3721 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
     3781 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
     3841 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
     3901 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
     3961 gtcaattgct aatacatatt tcactttaaa tgtccccctt tattttcaga attaataaat
     4021 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
     4081 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattaaaaaa
     4141 gtatttttat tgaaatattg ttgcaatatt tattatttta ttctataatc tagttaaaat
     4201 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
     4261 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
     4321 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
     4381 ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
     4441 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
     4501 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
     4561 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
     4621 cccggaccta gacattttag aaaaatacat aattgaaaat tctgatataa aagtaattgc
     4681 tatggtgttc catgaaacta gttcaggaat gataaatccc gtccctgaac taggaaaact
     4741 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
     4801 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg
     4861 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
     4921 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
     4981 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
     5041 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
     5101 gaatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
     5161 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
     5221 aagggaaagt ggctatgttg tctatccagg aaaaggtaaa ttcctagatg ctaatatatt
     5281 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
     5341 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata
     5401 tatcagtata aattaaatag actgattttt ttaaattttc ctata
//
LOCUS       DAFKMX010000217         5428 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFKMX010000217 DAFKMX010000000
VERSION     DAFKMX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.221"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(227..790)
                     /locus_tag="LZB08_003349"
     CDS             complement(227..790)
                     /locus_tag="LZB08_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316814.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(951..1664)
                     /locus_tag="LZB08_003350"
     CDS             complement(951..1664)
                     /locus_tag="LZB08_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBM7316815.1"
                     /translation="MGKVAKLLLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1956..3620
                     /locus_tag="LZB08_003351"
     CDS             1956..3620
                     /locus_tag="LZB08_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBM7316816.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3727..4626)
                     /locus_tag="LZB08_003352"
     CDS             complement(3727..4626)
                     /locus_tag="LZB08_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBM7316817.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4653..5291)
                     /locus_tag="LZB08_003353"
     CDS             complement(4653..5291)
                     /locus_tag="LZB08_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBM7316818.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
BASE COUNT         1880 a          827 c          637 g         2084 t
ORIGIN      
        1 gtttgtacta tccatccctc acctaaagga gtgggatttt tgcactattc tgtattatat
       61 atatccaaaa atattataaa aatacaaata ttagcagaaa tttaaaaact gaagaaagta
      121 ttctaactaa gatttacaaa ttcttaatcc tatcgaagtt aaactactat cttcagccta
      181 attatctagc aatatttttt tatatgaaaa actctaaatc aagtttttaa atgtttgcga
      241 tttttgatac ataagaatgt tgtctagaat tatattctga taataaacct tcatactcta
      301 aggttaagcc agataaatta taaaaatcat ttggcgttac ttcaaatgct tgtttattta
      361 aaatgtctat ctcacttttt tctaacatgt cagcaacaaa atttgaacat acatatttat
      421 attcagattg tctctgaata ttcattagta aatatattaa tgccattgta tcataatagt
      481 aatcttctct ataatcacta attaatttta tatttttata tagattttca tattgtaaat
      541 tatctacttc taacgaatat accctacaca cagtgttttt tcttatagca tataatcctt
      601 gatttatatt ttcttcaaca aatcctccaa taaatggatt ccttggattt agccttccaa
      661 aagaatacat tggttttaga ttttcattta atgatattga cacatgtgta tacagctttt
      721 tacttatatt ccttattaag taagataata tagttcctgt atatgttgtt acaatataga
      781 ctttattcat catttccccc caatctagta actaaataat attatacact tacaatttaa
      841 tatcataaga tattctaaca taataaatgt ggttttacaa attttgattt taaggtactt
      901 taatattata taaaatataa taaaaatata agtataagtt ttctatattt ttaaccaaca
      961 gatgttttct tcaaattaat cagatgattt agtatatctt gaacctttcc agagtccttt
     1021 aaatatccta atgctccatt tatttgtgat tttaaatcta tttctacttc catatccaaa
     1081 tcaacatttc ctttttttat agatatattt ttaagtgtta ttgggtaaag ataacttttt
     1141 tccaaagtaa tcttatttcc attaacctca aatggaactt ttttattgta gttacttaat
     1201 attttttcta gcattttatc acttagttta aattttccaa ttttcacatt ctcaagactt
     1261 acaactaagt ttccatcctt taaagtaggt ctcagttcaa gttcatattg actatttacc
     1321 aaaccaaata ccttatatgg tcctgctacc tttattttac catttttcat ttcaacaaaa
     1381 ttattttcaa gttcattaat tccatgcttc tgcatcaatg tatatattaa atttttaaac
     1441 tcttcctctg aaacagatat ttttccaacc attctaagtg gattttttac tacctccata
     1501 gaatttatat atgagccttt tgataaatat tcttcatcta tgattttatt attttgtgaa
     1561 ctaacatcat atctttcttt tggtgtcaag atatatacaa caattcctgc aagtactaaa
     1621 acaataacaa ttaaacttaa caaaagcttt gcaacctttc ccataatcgt ctcctttaca
     1681 aatctgtcat cagatttaaa ataaatttag ataactaact tcacataata ttcttaacaa
     1741 tatacatact ataatacagt cttttaaaaa ttaattcaac caatctaatg ttttgtaaat
     1801 aaactgtaaa aacatgtttt tagtataaaa gttaatattt tgcataatac tcttaattac
     1861 acctttattt ttttctaatc ttggattaaa attaatatta tgcaatacag tgtagtgttc
     1921 tgtattataa tcaaattaaa taggaggtat agaatatgtc aaatgaaacg aactcatcta
     1981 actttataaa gaatattatt ataaacgact tagaaacagg aaaacatgac agtatcataa
     2041 ctcgttttcc acctgagcca aacggatatt tacatattgg tcatgcaaaa agtatatgtc
     2101 ttaattttgg attagctaag gagtttaatg gaaaagctaa tttaagattt gatgatacaa
     2161 acccattaaa agaggatgtt gaatatgtag agtccataaa agaagatgta aagtggttag
     2221 gatttgattg gaacgagtta aattttgcat ctaactactt tgatgaaatg tataagagag
     2281 ctttaatact aataaaaaag ggtaaagctt atgtatgtga tttgacacaa gaggaaatga
     2341 gagaatatcg tggtactcta actgaaccag gaaaagaaag tcctcataga aacagaacta
     2401 ttgaagaaaa ccttgattta tttgaaagaa tgaaaaatgg tgaatttaaa gatggtgaaa
     2461 aaacattaag agccaaaata gatatgtctt ctccaaatat aaaccttaga gacccaatta
     2521 tatatagaat atctcattca acacaccata atacaggaga taaatggtgc atatatccta
     2581 tgtatgcttt tgctcatcca attgaagatg caattgaagg aataactcac tctatatgta
     2641 ctttagagtt tgaagaccaa agacctcttt atgattggtt tgttaaagaa tgtgagatgg
     2701 aaaatatacc tagacaaata gaatttgcta gacttaatat aaataatact gtcatgagta
     2761 aaaggaaatt aaaacaactt gtagatgagg gtattgttga tggttgggat gaccctcgtg
     2821 ttcctacaat atccggaatc agacgtaagg gatatactgc agaagcattg cgcaatttct
     2881 gtagtgaaat aggggtatca aaagttaact ctacggtaga tagtcaaatg cttgattatt
     2941 tcctaagaga aaacttacaa ccaaaggctc ctcttactat gggagtttta agacctttaa
     3001 aacttattat aactaattac ccagaagata agattgagat gcttgaaata gagaataatg
     3061 ctaaagatga atctcaagga aagagattag ttcctttctc tagagaattg tatatagaac
     3121 aagatgattt tatggaagaa cctgttaaga aatattttag atttttccca ggaaatgaag
     3181 tccgtttaaa aggtgcttat tttgtaaaat gtactgatgt aattaaggat gaaaatggaa
     3241 atgtagttga aatacatggt acttatgacc ctgaaactaa gagtggctct ggctttacag
     3301 gacgtaaggt aaaatctaca atacattggg ttgatgctaa atcagctata ccatgtgaat
     3361 ttagattatt tgaaccatta attcttgatg atatacctga aaatgaaggt aaacatttct
     3421 tagaacaaat aaatcctaat tcattggaaa tattacaagg ttttgttgaa cctacacaga
     3481 taaaagatgc taaacctttt gataaattcc aatttgttag aaatggattc tttagtatag
     3541 ataataaata tacaactgat gaaaaatttg tatttaatag aatagttcct ctaaaaagtt
     3601 cttttaaacc aggtaaataa tcttacaatc aaaaaatgaa gacttaaaat ataaattact
     3661 tctactataa ataaacttag ttagaagtaa ttttttattt gagtcttcat catttacttt
     3721 aaatgattaa cttttttcac tacagattcc catgacttct attccatctt ttatgcattc
     3781 tatgttaaga ggaaaatctg gtcctttttc tccgtcaata tctgttataa gtgaatcttc
     3841 acattctata tgcacctttt tagttttaaa gtatagtatt tcatttccat tatattgatc
     3901 taaataatca cctctaagca cacttatcaa aactggtatt gattttggta ttggcattcc
     3961 tttaaaaata attacatcca aatatccatc atctatttca gccttatatg ctagatttat
     4021 atttcctgct gttttgccat taaaaatcaa cataagatac atatctccat cataatacat
     4081 ttcctcagaa gatactttta tcttaaattt cctcatatac aatgcttctt ctattccctt
     4141 tatataataa gaaattcttc caaatgaatt ttttaagtct gtgtttatcc tttgtgatac
     4201 atccgtaaac ataccagcac ttgcaacatt tataaagtac ttattattga ctttccctat
     4261 gtctattttc tttggttttg aatttatgat ttcatttata gcttctttta tatcaaatga
     4321 aatttgaaga gcatttgaaa aatcatttgc agttcctgta ggcaaaattc caattggaat
     4381 ttttatgtct aattctttca ttgcatttac tactaaatct acagtaccat caccaccagc
     4441 tatcaatatg tgttcataag aactatcaac ttccttaaat gcattcttta caggttcttt
     4501 aatagttaat ctatagggta tcaataaata atttttttct tgatataatt ctatgatagt
     4561 gtctaaattt gataatatct ttttttcacc tgaattagga ttgtatataa gttttacttt
     4621 tttcattatt tctccttact cattttattc ttttagtatc caaaatcttc atttaaaaaa
     4681 atcacatgta ttctttctgg tgttaattgt ctttttataa gtgtataact acagcatatt
     4741 cttcctggac ttgaacagtc tatacatata ccagtagtct tacatccagt atttgtactt
     4801 aatcttttac aattggctgg agcagctatt gtttttgttc tttttatagc ttcatctaca
     4861 tctttaacta ttttatttac tcctgctata actattactt tatcaggtcc atatatcata
     4921 gcagcaaccc tattcccatt cccatctaaa ttgtaaagtt ctccatcctc tgttatcgca
     4981 ttagtacttg taaaataagc atctgcagaa aatgactttc tatatatttc tttcatttct
     5041 tctggtttga tatcttttgc atatctatca agaaaattat atttttctct tataaaatca
     5101 atcactcctg tttcaaataa ggtcatagaa cctccacatc ctactagaga acctgcttta
     5161 actatttctt caattttttt aagaagctct tctttgttac ttactaaata ccctttcata
     5221 ttattgtttt ccaaagattt tattgttttt tctattcttt tttcattaag ccaatttaaa
     5281 ttttgatcca ttttaacttc ccccttattt ttaatatgta cttaattatt tataaacttt
     5341 attttcttta taagtttatt ttagtacatc taaaaaaaaa tacaatataa aacttaaata
     5401 gttttacata attttttcca aaataact
//
LOCUS       DAFKMX010000218         5383 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFKMX010000218 DAFKMX010000000
VERSION     DAFKMX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.222"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(525..1931)
                     /locus_tag="LZB08_003354"
     CDS             complement(525..1931)
                     /locus_tag="LZB08_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7316819.1"
                     /translation="MDSVKYILRRFIGSSILIALLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKYINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1976..2683)
                     /locus_tag="LZB08_003355"
     CDS             complement(1976..2683)
                     /locus_tag="LZB08_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316820.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEALPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2854..3768
                     /locus_tag="LZB08_003356"
     CDS             2854..3768
                     /locus_tag="LZB08_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316821.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNKGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3768..4529
                     /locus_tag="LZB08_003357"
     CDS             3768..4529
                     /locus_tag="LZB08_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316822.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4541..5278
                     /locus_tag="LZB08_003358"
     CDS             4541..5278
                     /locus_tag="LZB08_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316823.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1864 a          834 c          697 g         1988 t
ORIGIN      
        1 aaaatactac acaaaaactc tactttagta gaacttaatg gcgcaaaata cttcaattta
       61 aaacatttat cgtactttaa ataaataact ataataatat acttacaata gttacataga
      121 agctattata actacacaga acagcgtata attaaaactt ttttaggatg tcattatatt
      181 ttttcttaaa attaaaaaac actataccat aattctattt tagtagaatt tttttgtcaa
      241 taataaaatt aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata
      301 accttacaat aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag
      361 ccataagcgc ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat
      421 ttatccttat tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa
      481 atattttcat tcttcattct tatctgttat tattttataa aatatcactt aattagaggt
      541 aattttagta ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta
      601 tgtgcattaa taatgccaga tactatcaaa atacctagac catgattttg acctttggca
      661 tatgcctcac ttagtccatt ttggcccttt aataaactat taattctttc ttgactcata
      721 ccttttccat catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc
      781 cttatattac atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc
      841 ctttttaata attttttatc tgcatctata ataatatctt catttagtat atcaagttca
      901 acgtaaaagt cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac
      961 gcagaaactc taatcttatc acaattcaat gcttgcatat tatattcaag tttagacact
     1021 aaatttaggt catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact
     1081 tgctcttgtt ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt
     1141 atcattgaaa gtggagttct tatatcatga gatattcctg caatccagtt agacctagct
     1201 tcctctttct tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata
     1261 tattttgaaa tatcacttag tattcctttt tcttgtaaaa gtactggttc tccttttgac
     1321 ataagcttta tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat
     1381 agagctatta aaaaagtaac tattgcatta accaaaaaca tacctattat taacatagga
     1441 aattgttcta tttccttaac cgtataagta aggttaaact tagtgtactt gtcttttgga
     1501 taacctgtta ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa
     1561 aagaaccttg aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt
     1621 aaatcaaatc cccatattct atctccattg ttatcaatta acattgccca aactttattt
     1681 ttatgtagta cttccttact agaatcatct aaaataaact ttccatctct taaaaccaaa
     1741 ttatcttcaa tcataatcaa gtttgatttt atttctctgc tatcacttgt atctaaatta
     1801 tcagagtatt tagatacaaa taacatatat gaaaacagaa gaaaacctgc tatatttaat
     1861 acaataaaaa gtaatgcaat caagatactt gaacctataa atcgcctcaa tatgtacttt
     1921 acactatcca aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat
     1981 taatcatcaa cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag
     2041 ttttttctat cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat
     2101 acatatcatc tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat
     2161 tttcatacaa ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgcccat
     2221 ctttctcaac acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg
     2281 gcaacgcctc ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag
     2341 taagttcctt tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa
     2401 tcaatctatc ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct
     2461 ctctaatttt tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg
     2521 cacaatctgg ctttacattt ctaaataccc taacagcttc ctcataactt gatgctaaaa
     2581 atactctact aaatccctct tttctcagga tttcatctat catttttaat aattccttgt
     2641 ggtcatcaac cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt
     2701 agtataccat atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag
     2761 gtaaatttca gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc
     2821 aataagcaca gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga
     2881 ctttcaaagg aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa
     2941 ggagctgtat atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg
     3001 cttcttggac ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat
     3061 gaaaaaaata gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac
     3121 tatggtcatc ttacagggct tgaaaacatg accataatgc aaagactctt agatttacct
     3181 aaaaaaaatg taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa
     3241 gttagtcaat attctttagg tatgaagcaa cgtcttggca ttgctatggc aataatgaag
     3301 tttcccaaat tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag
     3361 gaaattagag aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct
     3421 agccatcttt taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc
     3481 gaactggttt ttcaagatag cataaaagaa cttcataata aaggtcaaag tcagattgct
     3541 atcaaaacta agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca
     3601 cttatacaag atgggtactt gactattgaa aatttaaaag actcagatgt ggctaaaata
     3661 aacggtatgc ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc
     3721 ttggaagata tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg
     3781 gtctagaatt ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgtag
     3841 ctgttgaaat aatgtggtgt atcataaatt caaatagagc tattacacgt aatccagata
     3901 tgttaggtgg atttgaatat tcctatatgc ttatgagttt tgcaagttta aatggtctat
     3961 tctttcctat cttaatatca ataattacat cacgtataag tgatatagaa cataaaggag
     4021 atacatggaa gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat
     4081 ttttatgttc tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt
     4141 tgtttggaca ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca
     4201 cattaggaac aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg
     4261 taattccaaa tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga
     4321 catcaggttt gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt
     4381 tatcaccttt aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata
     4441 tgaatttttc tattacgatt ttagttttct taattggaat gctaatttat tatattggaa
     4501 gaaatcattt attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtttt
     4561 gggagaaatt ctaaagttaa aaaattcttt tatatggtat gtattactag ctttgccact
     4621 tataagtgtg ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga
     4681 atggtacagc ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt
     4741 aatcgcaatc ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa
     4801 tgtaatgaca ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc
     4861 aatacttaca gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat
     4921 gtttggtttt tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat
     4981 tatttctatt tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc
     5041 aactcctatt ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa
     5101 acctgctgtt ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa
     5161 gcaatctgct ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac
     5221 tatagtattt tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg
     5281 gtaaatgcta gatatttttg ggtttgaaat ttatattctt tttagttgaa atctatatcc
     5341 attttattta aaaatatata tcttttattt gaattttata tgt
//
LOCUS       DAFKMX010000219         5356 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFKMX010000219 DAFKMX010000000
VERSION     DAFKMX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.223"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..107
                     /locus_tag="LZB08_003359"
     CDS             <1..107
                     /locus_tag="LZB08_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7316824.1"
                     /translation="EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            234..1136
                     /locus_tag="LZB08_003360"
     CDS             234..1136
                     /locus_tag="LZB08_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBM7316825.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKDRGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            1748..2761
                     /locus_tag="LZB08_003361"
     CDS             1748..2761
                     /locus_tag="LZB08_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBM7316826.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3113..3682
                     /locus_tag="LZB08_003362"
     CDS             3113..3682
                     /locus_tag="LZB08_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBM7316827.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(4018..5355)
                     /locus_tag="LZB08_003363"
     CDS             complement(4018..5355)
                     /locus_tag="LZB08_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBM7316828.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT         1967 a          541 c          900 g         1948 t
ORIGIN      
        1 gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa
       61 atatattaaa agaaggatta agactaataa caattcaaaa taaataactt atgtaagaac
      121 cgtaggaact acggggatag cctgtggaga attagtaaga catatgttag tatgcaaaat
      181 tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattgga aatgtgttgc
      241 cacaagatta ttatggatta atagtgattt taggtgttat tcttggagtt atgggggcat
      301 tttacaactg gtttactcta tatgtgcagt ctttatacaa gaagattcct aaaattggta
      361 cctttggaaa actgttgatt ccatttataa tggctggtat tcttgggctt gttatgcctg
      421 atgttctggg gagcggacac aatttagttg aagaacttac aagtcatgaa tatatttttg
      481 gaatggttgt tttgattttt gttatacgtt ttatattttc ggctgttagt tttgggtcag
      541 gtgcaccagg gggaatattt tttccattgc ttgttttagg agcatttata ggtggaatat
      601 ttggtatggt aggtgttaaa gtttttggaa tgaatcccga ttatgtaaat aactttgtgc
      661 ttttggctat ggcaggatat tttacagcta ttgtgcgtgc ccctttgact ggtatcatat
      721 taatttttga aatgacaggt tctgttagtc aaatgctttc actatcagta atttcaattg
      781 tggcatatat tgtggctaca cttatgaagt ctaaacctat ttatgaaagt cttcttgaga
      841 ggttacttga aaaaaatgga gaacctgtac ataaagatag gggcgaaaag atactagacc
      901 agttctctgt tatgtttggc tctcctgttg cgaacaaggc aattcaggat gtacagtggc
      961 ctgaaaattg tcttttggta gcaattcaaa gaggtggaga agaaattatt cctagaggta
     1021 agacaattct tagaccaagt gatgtaattg tgactatgac agatgagaga gatagtggtg
     1081 ctgtgtatga taagatggaa aatttgtgta aagaacatac ggcacatatt ttttagggag
     1141 aatgatgaat gtttaataat gatagaatat ttaatgaaga tttgattaaa aaattggtaa
     1201 tttgaaagag agttaaggaa ttaatataag aaaatgaaaa tgtgatatct tagatgtaaa
     1261 agagtttgaa gaaaataatt gtatatattt gttaatgaga ttatatggac taatggtata
     1321 tttatactgt gctaaaagtt gtactattgt atgaggaatt ggaataaagg gtttgaaaaa
     1381 ataggtagaa gatttttcag atattcttat tgggatattt taattaatat attttaattg
     1441 cgatattttg ggaagaatag aatttattta taaatgaaaa taattaaaag gggttaaggt
     1501 tgtattagcc ccttttgctt atagagtttg gaaatggtat cttgatagta gttgtttcat
     1561 gcttattata ttttattaca tattttaaaa ttgaccttat tactcctaaa tcattgttat
     1621 atcaactttt aaaacacttt caagacaatc gcctattgaa aacaaagtta attaaaaacc
     1681 tacatatcag tttagtattc gtgtagatga ttatataaat ataaaagtat aagaaaggtg
     1741 agtatctgtg gttagttcat ttttaaatga tatctttaga acaattttag taagttgtat
     1801 ttttatagtt attttattag tctttagaat aactttattt aagattttca gtaaaaaatt
     1861 taattactat atatggctta ttgtgattat taagctatca ttaccattta tgaattatac
     1921 ttttatattt aatgagtcaa aatataatgg taatattaat aagataagtt taggcaattt
     1981 taatagtatt tctattgtat atagtactgt tttagtctct gtttggattg ctattacgat
     2041 attttattta gtttatactt tattgaaata tattaagctt aaaaatatga taaatgattt
     2101 gtcttatgat gttgaggatg ataagataaa aaatctttat gaaaatttat taaaagagct
     2161 taagataaat aaaaaaatac ggcttaaata ttcatatgaa gttgaaagtc cagtattttt
     2221 taattcgtgt gtcattttac caccatgtga ttatacatta aaagagttaa actggatatt
     2281 taggcatgag ttgatgcatt ttaagagtaa agacctttat attaagtatt tggtgatatt
     2341 tttaaaatgt gtttattggt ttaatccttt tgtgtatata atggagaagt ttatagattt
     2401 ggattgtgaa ttttattgtg atgaaagagt tttgaagaat tgtacgatag aagagaagca
     2461 ggattatgca ttgacaataa ttagtgcaat gagaaaaggt tcaaatagtt caaataaatt
     2521 tgtggctgga ttacataagg agtcagatat taaaaaaaga gtgtttagta tgtttaatga
     2581 aaagtacaga aatggtattt tgatagcagt tattttatgt ttattatctt ctattacata
     2641 ttttaaaatt gatttaatta ctactaaatc aatattttat ccactttcaa aacaaacacc
     2701 tattgaaaac agaactaatt caaaacctac atatcagatt aatgtttata tagataatta
     2761 aaaaatacaa agtttaacaa gagattaata taaataattt taataagtat ctttcacata
     2821 tgttaaattt actttaatta tattaaagcc aatgaattat tctttagaag ctccaatgcc
     2881 agtaaataca ggtaaagaaa aaattactac aattgtagat taaaaatgtt ctaatattta
     2941 aagaaatgca aaagtgtaag aagggtaaaa atctgttata atacattaaa aactaaattt
     3001 agaaaaattt acataattag aaaaatgatt tgataaaaaa taaagttaaa tattaactaa
     3061 gtaaatttga aaaagaaaaa atataaagta caaatttaaa aggaggtaac taatgaaaaa
     3121 gaagttggaa gatatgactt tagaggagtt atggaacttg tttcctatat ttttgactac
     3181 tcacaaagaa tgttggaaaa attggtttaa agaagaacaa tatagactaa ctggaatatt
     3241 gccaatgcaa aaaattgtta gaataagtca tattggaagt acttctgtga gtacgattta
     3301 tgccaagccc attgttgaca tactgattga gataattcct gattataata tgaaagatat
     3361 taaagatttg attgagtcga atggatatat ttgtatgtct gagagcagta gtaggctttc
     3421 acttaataaa gggtatacag tagatgggtt tgaagaacga gtttttcatt tgcatataag
     3481 gtatgtaggt gataatgatg agttgttttt tagagattac ttaattgacc acccagatgt
     3541 tgcaaaggag tatgaaaaac ttaagttgga cctttggaag aagtatgagc ataatcgtga
     3601 tgcttataca gatagtaaac tagattttgt tagggagttt actgaaaagg caaaggtgtt
     3661 gtattctaat agatataatt agaaataggt acaattagat atttatctta tatattgtat
     3721 tatatttttt aagaaagtaa ttaatagaaa aagggcttgt ttcaaaatgg agagataaat
     3781 ccctttttca ttgttgaaag gataaaaata tatttgattt catgtaaaaa agagtgcctc
     3841 atcaactaaa aagtttattt tgagacacac acatttttac taaggtaaat aaaatatcac
     3901 atttgattaa attttaagtt aatgaatata tgtgtaaata tttaattata agttattata
     3961 attttacata gtatcaaaca atctaatttg tattatataa gataatctaa ttttagacta
     4021 aaataatcta atataaagaa ataaagaaaa ttcactctca gtataagaga tatccaattt
     4081 tccattgtac ttattaacaa tttctctaat attctcaata ccatatccgt gagaattact
     4141 atctttttta tctgaaataa atagttcttg ttttctattg ataggattta ctttgttgtt
     4201 tgtcaataaa tagctaaaat actccttctc acaacgtgcc tttaatacca cttttctatt
     4261 tgacaaatca ggaattttca aagatgcttc tatagcgtta tctaaagaat ttgaaaaaat
     4321 agtacataaa tctataggtt ctatacccaa gtgattgtct atatctatat taaagtgcac
     4381 atcaacacca ttttcttcca aacttgtatg acgaagattt aaaattgagt taagagcttg
     4441 gtttttgcaa aattgtttgc cactaagagt acctatacct atttttaact gattaaaata
     4501 ctttttagct ccagcaatat cttcaatttc taaatacgat tcaatcattt gaaagtgatt
     4561 attcatatca tgtcgcattt tgcgtatttg aatttgttga ttttctagta agttatagta
     4621 tttttctttt aggtgatttg cttgtttttc tatcttcatc tttacatttt caatcaatgg
     4681 ctgtaaaaga agtatcacac cgatatttgc cagaattgct gacaagatga ttagataagt
     4741 gccataaggt aagttgccta agataatatt cttattacta cccataggga ttaaattagt
     4801 aggtggcaga ataatagcaa tcaatattga taaaaatgaa gatatacaca ccatatcaat
     4861 aaaaatccat attttatttg ataagtattg ttttatattg accaatctat tctttaggaa
     4921 aatatgtatt aaaaaccaaa ataataattt aattagagca gaaattatct ggactattga
     4981 aagtattaaa atatactctt ttgagtgtat aagttcctca tatatagggt tgtttataaa
     5041 aagaaaatta agcccaatta taataggata aagtatcatg acaactgata ttataggaat
     5101 taatttgctt ttgctacata ttataagtag tataaaatat ccgaatagtg taaacaaaat
     5161 atttacgggg tcggaaagcc atattatcat ttgactaata atagagtagc ccaaaatcaa
     5221 tatgattttg aaaatccaat gattcctaaa gtctataaaa taggaagtca acttataaag
     5281 aaaaaaggct atgaaaatat tgattaatat tagaataaaa tttatgcttg tagagattaa
     5341 agaataagtg cccatt
//
LOCUS       DAFKMX010000220         5251 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFKMX010000220 DAFKMX010000000
VERSION     DAFKMX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.224"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(106..1290)
                     /locus_tag="LZB08_003364"
     CDS             complement(106..1290)
                     /locus_tag="LZB08_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBM7316829.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLIKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1663..4014
                     /locus_tag="LZB08_003365"
     CDS             1663..4014
                     /locus_tag="LZB08_003365"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBM7316830.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKKKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4036..4575
                     /gene="nrdG"
                     /locus_tag="LZB08_003366"
     CDS             4036..4575
                     /gene="nrdG"
                     /locus_tag="LZB08_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBM7316831.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2053 a          593 c          842 g         1763 t
ORIGIN      
        1 ttttatctaa catttaaatt aagttgttga atatatctta tattacttac cttatatatt
       61 aattaccatt aatacattat atctaattac tattcatcta aattattatg ccattatttt
      121 atatacaaat tcttttaatt tatctaatcc agataagaaa gtattttcac tgcaagcata
      181 ggatatacga atatatttat ccataccaaa tgctatgcca ggaacaactg caacattata
      241 ttcttctaaa aattgattac aaaattctat agaaaaacta tctttatatt caaatttctc
      301 agatacttta gaaagatcta taaatatata gaatgcgcca tttggattca catatcctac
      361 attatcaata gagtctagtt ttgatttaat taaatctctt cttgatttat atgttttaac
      421 catattgtct atgtcaattg agcaatcttt caaggctcca taggctatgt attgtgctgt
      481 tagacttggg tgagatataa gatgtccttg tatagaactc attgctttag caatcgtttt
      541 attagatgca gtatatccta atcttaatcc agtcattgca gctgacttag aaaaaccatt
      601 tatagttatt gttatatctt tagcttcttc gcttaatgat gcaattgatg taaattcacc
      661 agtgtagcat attttttcat atatttcatc tgctaaaata tatattttat tttgtatgca
      721 tacatctact atttctatta attcatcttt tgtataaaca ctaccagtag gatttgaagg
      781 attgtttatt acaagaattt ttgttttatc tgttatagat ttttcaagtt cttcttttat
      841 taacttaaat ccattttctt ttttagtatc aataaaaact ggaacagcat taactaactt
      901 aatcatttca ggataactaa cccaataagg ctttggtaat aagacttcat ctccttcgtc
      961 tgttaaagct agtaaagtat ttgttattga gttttttgct ccacttgata caactatttc
     1021 gtctattgaa tagttacagt tattttcttc aataagtttt ttacagattt cctctctaag
     1081 tatttttaat cctggaacta aatcatattt agtgtaatct ttatttaaag aatcaatacc
     1141 ataagatttt gcgttattag gtacattaaa atcgggttct cctatactaa gatttattac
     1201 tttaactcca ttactttcca tttctttaac tttagaacta atacctattg tgtaggatgg
     1261 tgttataaag tttagtcttt ttgataacat aaaatgcctc cttttaaatt attatttata
     1321 ttatacgaca aatttaaaga atttacataa ggaaatttgt agatatatac attaatatat
     1381 ataatagcct acactttttg cctaaaatta gtgtaaaaaa aagagaaaaa aattttaact
     1441 ttcaaagttg ttagaaacgc tactgttcaa aatatccaca tagtatttgc aatgatttgc
     1501 gcaagtttga ctattatgta caaatttggt aatcttaata tagcaattat taagataatc
     1561 cctttaactt agttgaaggg gattttttta gacttaacat actacatata gtggtatggg
     1621 gattaataaa atacaatata tagtaacagg aggttttttt aattggttga attcgtaaaa
     1681 aaaagagatg gaagagtaat tccatttaat gaagatagaa taacaagagc tatattttta
     1741 gcagcaacga atgttgcaga aagagaagga atagtacctg attataaatt atctgagcaa
     1801 ttaactcaag aagtaataaa gtttttaaat cataaatact cagaatcagt tccaagtgtt
     1861 gaagatatac aagattctgt agttaaggtt cttatagaaa ctggccatgc aaaaactagt
     1921 gaagaatata taatatacag aactgaaaga agtagaataa gaaattcaaa aacaaggttg
     1981 atgaaggcaa tagaggaaat tacctttgaa gatgcagaag atgctgatat aaaaagagaa
     2041 aatgcaaata ttaatggtaa tacagcaatg ggaactatgt tacaatatgg aagcactgta
     2101 tctaaagaat tttgtaaaac acatatatta aaaccagagc attcttttgc tcatgataat
     2161 ggagatatac acatacatga tatggatttt ttaaatatgg gaacattaac ttgttgtcaa
     2221 atagatgtta aaaaactatt caatggtgga ttttcaacag gacatggctt tttaagagag
     2281 ccacaagata taataagtta tggtgcatta gctgcaattg ctatacaaag caatcaaaac
     2341 gatcaacatg gaggtcaaag tataccattt ttcgattatg gattggctga aggtgtatat
     2401 aagacattta agaaattcta cataggaaat ttagctaaag cattgaagtt atttaaagga
     2461 attgaaaata atgatgtgat aaagaacata gtatataata cagagaaaga aacaaatcaa
     2521 aaagttggtc ttaagagaga tgaattgtat ctaaacttag aaaaaaagaa attaatacag
     2581 acttttgata tagatgatga acttgtaaat aagatgcaaa actttgcatt tgaagaatcg
     2641 tatagagaaa ctgataaaaa aacatatcaa tctatggaag catttataca caatctaaac
     2701 acaatgcact ctagagcagg tgcacaggta ccattttcaa gtgtaaactt cggaacagat
     2761 acttctgaag aaggaagaat ggtaactaag aatcttctat tatctcaaga aagaggttta
     2821 ggtaatggag agacaccaat ttttccaatt ttaatattta aggtcaaaga aggcataaat
     2881 ttaaatcctg aagaccctaa ttatgattta tttaaattat catgcagagt atcagctaaa
     2941 agactattcc ctaattttag ctttttagat gcgcctttta atgcaaaata ttataaaaaa
     3001 ggtgaaccag atacagaggc tacatatatg ggttgtagaa caagagtact tagtaatgtg
     3061 tgtggttcag aaacagttag tggtagagga aacatatctt ttactacagt aaatttacca
     3121 agattgggta taaaacatgg tattataaat aatgaaaagg ctaatttaga tggtttcttt
     3181 gaagaattag atgagaagat aaacttaatt atagaacaat tattggagcg ccttgaggta
     3241 caaggaaata aaaagatgaa gaattttcca ttcttaatgg ggcaaggtgt atggaaaggt
     3301 tctgatgatt taggaccaga agatacacta aaagaagtaa taaaacaagg aacattgaca
     3361 attggattta taggtttagc agagtgcctt attgctctta taggtaaaca tcatggagaa
     3421 agtaaagaag ctcaagaatt aggattaaaa atagtgtcac atatgagaca taaaatggat
     3481 gaagcaacgg acaaatataa attaaatttc tccttaatgg gtactccagc agaaggtctt
     3541 tcaggtaggt ttactaaaat agataaaaaa gtttatggag aaattaaagg tatcacagat
     3601 aaagaatatt atactaattc attccatgta ccagtttatt acaatataag tgcatatgat
     3661 aaaattgaga tagaagctcc ataccatgaa ttgactaatg caggtcatat aacatatgta
     3721 gagttagatg gagatccatc tgataattta gaagcttttg aaactgttat aaaagctatg
     3781 aaagatttgg gtataggata tggaagtata aatcatcctg tagatagaga cccaatatgc
     3841 ggtttttcag gtgtaataac aagtaatata tgccctgtat gtggaagaaa tgaagatgaa
     3901 agtgacatca aattcgaaag aataagaaga ataacaggat acttagttgg tacagttgat
     3961 agatttaata atgctaaaaa agcagaagta agagatagag taaaacatag atagttttat
     4021 ttgggggaat aagttatgaa aataagaatg tcatctacta taagttatga cagcatagtt
     4081 gatggaccag gattaagaat ggtaatatgg actcaaggat gtattcataa ttgtaaagag
     4141 tgccataatc ctcagacaca tgatttatgt ggaggatttt atatggatac agaagaaatt
     4201 ataaataaag tcaaatcatt gaaattacaa aaaggcatta cgctatcagg aggagaacca
     4261 tttttacaac cagaaccatt agaagaaata gcaagagaag ctaaaataaa tgggttagat
     4321 gtatggtcat atactggatt tacgtttgaa caactgttag ataaaaaaaa tagagcgtat
     4381 tttaaaaatt taaatttact aaagcaaatt gatattttag tggatggaaa atttatagct
     4441 gaaaaaaaag atataagctt aaagtttaga gggtcatcaa atcaaaggat aattgatgtt
     4501 caaaaaagtt tgaaatataa aaaagtgttt ttggttgagc aatatatgaa ggatgattta
     4561 tctatagcag aataaaatat aaataaacag gattaaatat atgcatatat ttaatcctgt
     4621 ttattttcat ataatatttc aagcttgtat aatgtaacaa ttaaaccaaa agactcattg
     4681 tatatttgaa attaagtttt ttatagttta taggtgtttt atgtggatta cgaatgatat
     4741 agtatcgata cattatttaa tagattctta tgccaagaaa caattaattt atagaaattc
     4801 ttaattgtta caatataaat atctagatat gttataagat actgataaat aatttataaa
     4861 gtttattaat ctaatattgt gtgtgaatat taagtggtca agtagtctaa aaaatgaaaa
     4921 agatttaaaa aagtgtttta actaaacaca taagtgttaa attcatgtat aagtataggt
     4981 tagatacata agaaataatt atgtaatatt tatctttgta aaaaaatatt aaattttgta
     5041 atttgtggat aaatatgttt ataatgtgga taatatttaa tgtaataagt taaagcaata
     5101 tgaatcaact gtttataatg tggataatat tataaatgta tgaataattg cagtatttaa
     5161 aaacaaaaaa agttaaaaaa gttgttgact ctaaaagaaa aagttggtat agtaatactt
     5221 gtcctcaaga aaagggcaaa caaataaagc a
//
LOCUS       DAFKMX010000221         5221 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFKMX010000221 DAFKMX010000000
VERSION     DAFKMX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.225"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            209..709
                     /locus_tag="LZB08_003367"
     CDS             209..709
                     /locus_tag="LZB08_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBM7316832.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            766..1752
                     /locus_tag="LZB08_003368"
     CDS             766..1752
                     /locus_tag="LZB08_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBM7316833.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1802..2524
                     /locus_tag="LZB08_003369"
     CDS             1802..2524
                     /locus_tag="LZB08_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBM7316834.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2553..4487
                     /locus_tag="LZB08_003370"
     CDS             2553..4487
                     /locus_tag="LZB08_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7316835.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4480..>5221
                     /locus_tag="LZB08_003371"
     CDS             4480..>5221
                     /locus_tag="LZB08_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316836.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLAR"
BASE COUNT         2177 a          389 c          901 g         1754 t
ORIGIN      
        1 agtttgtata taattttaat atcatatatt ctatatatat aaaattgaat ttgtatgtat
       61 tatgttgtat aatataaaat gaattaggaa aatagttcgc taaaataatt tgtttttata
      121 aaattactgt ttaagtttat agattttgta taaaataaag ttatgttatt aattttttta
      181 gaaataaaac ttttactagg agacagatat gagaaaaaat agtattaata tagcactaac
      241 tgcagcaatt acagcaatct atgcagtttt aacattatca ttgggattta taagttatgg
      301 accaatacaa tttagggtag cagagattat gatgcttttg gcatttttag ataaaggata
      361 tatagtaggt cttacattag gttgtttttt agcaaatgta ataggaccat atggtgtacc
      421 tgatataatt tttggaacat tagccacatt tataagtgca agtatgattt atattactag
      481 aaagttaggt aaacagaata aaagtacttt agttatagct tctttatggc caactgttat
      541 aaattcgatt gtggttggtc taatgttaaa tgtattcttt gggcttccat taattctatc
      601 tatgatacag gtaggttttg gagaatttgt agtagtaaca attgtaggag taccattttt
      661 ctgcttttta atgaaaaaat ataaaaatgt cattaatatt aaattttaaa tagtgtatag
      721 taatttaatt aattgagagt attattagtg aaaagaggtg tttatttgtc gaaggtaaaa
      781 agattgactg atgtttggat atatattaat gataagcaaa agtttacaat aaaagagttg
      841 tcagaggaat ttaatctttc agctaaaaca atacaacgat atttaaaaga attaaataaa
      901 atgggacttc caattcaagc agaacaaggt agaaatggag gttatagggt gctaaataat
      961 agctatatac ctcctgttat atttacagaa aaagaagtta tgattatact atttgcactt
     1021 aagtcacttc agatttataa ttataagtta atggaaatag aaattaattc tataatgaga
     1081 aaagtaagtt atgaacgtaa aagcagtata aaagtaggta tagaaaatat taaaagatac
     1141 attggctttg tagctaatga tactaatgat ggagatttta agagtagtaa tgtaactgag
     1201 ttttttagag cttcttcaga atctctaatt ttaaatatta agtataagtg tggtaatcag
     1261 attttggaaa aaaacgtatg ccctataggt atatatttaa ataagggagc atggttttca
     1321 ccagcatatg attgtaaaag tgataaaata atattagttc atatagataa tataattgat
     1381 ataaaaagaa caggagaaag taaaaaaata catataaact tggaagaatg gttacatgaa
     1441 gtatataaca atttatatgg aaatagttca actactaaaa aatttggtga aattatttat
     1501 ctaaatgtat tattaactaa agaaggtgtt tctaaaataa aggacagttc ttggaattta
     1561 gatatggagc taaatgaaga tggtagtgga ataattagta cattcataaa ggttgaagat
     1621 atagattgtt atgcttcgat tttatttttt attggaaata atgctaaggt tatagagcca
     1681 aaatttttga ataagctact ttatgataag gccaatgagt taagatattt ttatgaagaa
     1741 aatatggtat aatatttaga aatgttgaaa ttaaataatt attaaggaga tatatgttat
     1801 aatggaaaat tttaataaga atgctaaaat aattggtatt ggagctgaag gtattaatat
     1861 aataaatgaa gtagaagaaa aaataaaagc aaatatggat atagaaaaaa taaacataaa
     1921 tcaagagatt gaaaaagaat atgttagaag tcttttagat ggtgtagata tattattttt
     1981 gatatattca tctgaagata aacatattag agatattata aaagctgttt catatatgtc
     2041 aaacgaaaga agagttctat ctataggaat ggattgttca gagaaagaaa ataaagaaga
     2101 tttagagctt gggagagagt ttaaaatcaa taatgatagt atatttaaat ttgttgattt
     2161 aatgaatatc atggtagagt ctatttctga ttcttgcatg ataaatatag atattactga
     2221 tttaaaagaa gctattgtag gagataaagg aattaagtat tcttttgaag agtttgaaga
     2281 tacaaagtca tatagcgaga tagctgacat tttatttgac aggatggaat atattggtga
     2341 ggaatttata agtaagaaag gaattgtatt tgttgaagga agtccagaat tttctataat
     2401 ggaattgaat gatttaataa gtaatattca gagcaaggta gaagaaagct atgaagtaat
     2461 attttcttta tatataaagg aaaatttaaa tggaaatatt agagtaggct tattgtataa
     2521 ttagtatatt ttagatatta aggagagtgt atatgtctga aaatataaga ggtacagagt
     2581 attggttaat ggaagaattt ttttatcagg aaacagacac accacagatt tttagagaag
     2641 aaagcataat tgcacttgga tttgatataa aaataaattt aaaaaaagta ttcagtatgg
     2701 ggaaaaaatc agaaaagtat attaatgaga attgtaatga taaagatttt ataaaaaagt
     2761 ttatgtcgat taaaaaagga gattattttt tatataaagc atatcaaaat atagaagaaa
     2821 aagttaaatg taatgtgtct attggcatag ttgaagaaga ttttgaaaat ggatatgaat
     2881 taagtcatat tttagggcat acactaccag taaaatggat gcataatttt gatacagttt
     2941 caaattgtaa agtatataca agtgaatttt ataaattaaa taataatgat attagctcat
     3001 atttagaaat tattaagtca caagataaga aattattgag aaaaagaata gaagaaagtg
     3061 aaaataataa atggtattta aaaaactatt attataaaaa tgtagataag tgtgtagatg
     3121 atgaagaatt atttattcag cccattaatc tagaacaatt ggaaagccta aaaaatataa
     3181 agacagatga ttatatttta ttgtataata gcgagaaagt aatttgtgaa aatgaccata
     3241 caatgaagag tctatttggc gtatgtcaag ttatggaagg gttcgataaa gagaaagtaa
     3301 gtgctttgga aggtggatat ttttttcctg ttagatggat agagtatgaa aaggagaata
     3361 tagacggaaa tgcatgggtt atatttgaaa ataatagtat agttgaaaag tatgtaaaaa
     3421 gatttactaa taaaatgcca ttaaatacta tactttatgg accaccagga actggtaaaa
     3481 catacaatac aaaacagatg gtttacaatc tagtaagtga aaccagtcta agaaacaaat
     3541 actatacagg ttacttagat tataaaaaca atgtagaatt ttgtacattt catcaatcat
     3601 atggatatga ggaatttata gagggtctta agtctgatgg tgaaggcaat tttaaaccag
     3661 aagatgggat attaaaggat atatctatag aagcatgtta taatgcattg aaatttgaaa
     3721 aaaagatgag ctttgagttg gaaaaagata gcttgacttt agaaactata aaagacagga
     3781 aaaagaaatt agtattagaa aatatagaat atagtgagag ttttaatttt gaagaatcag
     3841 aaaactatgt tttaataata gatgaaatta atagagggaa tgtatctaaa atatttggtg
     3901 agctaataac attattggaa gatgataaaa gacttactaa atcaaatcaa actattgtca
     3961 aactaccata ttcaaaggaa aaattttctc tacctccaaa tttgtatata gttggaacta
     4021 tgaacacatc agataaatca atagcacttt tagatatagc tttaagaaga aggtttaatt
     4081 ttgaagaaat tatgccagat tatgatttat tgtctattgt agatggtata gatattagaa
     4141 aaatgctata tacaatcaat aaaaggatag agtttttata tgatagagat catgttattg
     4201 gacaagctta tctacttgct gtagaaaata ttaatgatat agtagtagta tttagaagta
     4261 aaataatacc acttcttcaa gagtattttt attatgattc agaaaaagta ggtttgattt
     4321 taggaggaat tggaagaaat gaaaaagata aatacattgt ttataaagaa aaacttatag
     4381 cttctgagtt atttaaaaat aataatgcct caataatgga gagtaaagtt aactattgta
     4441 taaaacaaaa catatctagt gaggagctaa aaaatatata tgagtaaaaa tatatatgtt
     4501 aaggaaacct atgaatggat aagggtaggt aatggtgaaa atgagcttac agaaattgaa
     4561 tatgaaaaat tgcttaaata tctagagaac aataatgatg tattaaaaag taatataata
     4621 gatattaaat acaaaaaact tagatttata aactatgtag gcatcatatg ttttgaaaat
     4681 gtaatacttg aaatacttcc taaattgtca ttaagtgata atcttgtaaa agatagagag
     4741 atactattac agatgctttc tatatgtaat aaaattccaa ttacaatgaa tgaaaaaata
     4801 agattgagtt tgaaaaatta taatctctta aatttttttg ttatgtattt cattgagagt
     4861 atgcaaacac agatgaaaaa aggtatttat tttgaatata taaataaaat agagaactta
     4921 aatgttatga ggggtaaaat attattaagt acatatgcaa aagaaaaagg catttctcca
     4981 atgaaaataa gatgtgaata tgatgagtat agtgaaaata atttcttgaa tcaagttctt
     5041 aaaaaagcat gtatatctat tttatgtaga ataaatgaca attcaattca aggcaagatt
     5101 aaaaaaattt taagctattt tcaaaatgta gatttaattt atatagatag aaaaaaacta
     5161 ttggattata aattttataa aaacaatgat agatttaaag attgttattt actagctaga
     5221 c
//
LOCUS       DAFKMX010000222         5121 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFKMX010000222 DAFKMX010000000
VERSION     DAFKMX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.226"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            625..894
                     /locus_tag="LZB08_003372"
     CDS             625..894
                     /locus_tag="LZB08_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBM7316837.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(1112..1357)
                     /locus_tag="LZB08_003373"
     CDS             complement(1112..1357)
                     /locus_tag="LZB08_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBM7316838.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(1672..2052)
                     /locus_tag="LZB08_003374"
     CDS             complement(1672..2052)
                     /locus_tag="LZB08_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7316839.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
     gene            complement(2253..3446)
                     /locus_tag="LZB08_003375"
     CDS             complement(2253..3446)
                     /locus_tag="LZB08_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBM7316840.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(3509..4765)
                     /locus_tag="LZB08_003376"
     CDS             complement(3509..4765)
                     /locus_tag="LZB08_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBM7316841.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1815 a          861 c          598 g         1847 t
ORIGIN      
        1 aaatcattca atatattaaa taaatattta actttaataa tctatattta attatagcta
       61 taacatagaa ttatcaaata tattgaatga atatttgact cttggaatgt gtaattaaat
      121 taagtttggt tattcgatgg aaggcaaatt tgatataaaa ctccatttaa agtgccagat
      181 aaaaacttta tggttatata aaagttaaaa gtaatttata gtgttataga acattcaaag
      241 ggatttaaag taatctatgg tgttttgaaa tattcaaagg gatttaaact aatctatagt
      301 cttgtagaat attcaaagag atttaaaaca atctataatg ttgtaaaata tattcaaagg
      361 gatttaaact aatctatagt cttgtagaat attcaaagag atttaaagca atctataatg
      421 ctgtaaaata tattcaaggg gatttgaagt aatttattgt gttttgaaat attcaaaaag
      481 atttttggaa tagtttttat aaatttacca gtactaataa ctttatgttt aacatttaga
      541 tgacctaaaa tcaactattt gcacttttgt attcatctaa aatcatttta taatattttt
      601 aaataatgga aaggatgttt gaacatgttt gaatcaagat gtggtgtctg ttgtgacagt
      661 tgtacaaaaa aagaacaagt aaattgcact ggttgcccaa ctatggaaaa accattttgg
      721 ggtggagaat gtaaggttaa aacttgctgt gaaagtaagg aacttaatca ttgtggtgaa
      781 tgtgatactt tcccttgtga tatgctttta aataagggaa aagaccaagg ttttgaccct
      841 atggtaaata ttgagcaatg tagaaaatgg cttgaagaaa cagtttctaa ttaattgttt
      901 taaaatgctt tgttaagtaa tttatttaat cctttgttag gtagtatgtt tagtcatttg
      961 ttgattggta tatttataca tttattgatg ggtatattta gccatacaaa tagatttaca
     1021 taaaatttac gactaaaaaa gagttgagta ttagttttta ttatttaaaa cggatattca
     1081 actctttttt atgtctaact tttttgattt tttaatcttt ccaataccac cactttaatt
     1141 tttctggttc tggattttca gttgatgcaa aatatactgc acatctgtat acatataact
     1201 gacatctgtc tatgtgctgt ccacgcatta aacattcctt ttcatacatt tgtgctgggt
     1261 ttgcatcttt aagtgattta atagttgtat atcctaacat gattaaagat ttttctgttg
     1321 ctttccctac ccctggaatt ttttgtagtt ctcccatact tcaccttctt tctggtatat
     1381 tctttttgta ttatacttag tcccagatat ttttttatta tagttaatat ttaatttttt
     1441 attatatcat atcaatttat ttatgctttt tcatatatgt atattttatt actacctcta
     1501 aagtactgag tcacttaatt acatcataat acagttttat acaaataaaa taatatattt
     1561 attttttatt ttgctaatag caaattattt taaattaaat ctacacattt aaaaagagtt
     1621 ttataattgt agataaacta ttacaaaact ctttttagac aacaaatcat attatatata
     1681 agcttcttcc caatttacta cccagttttc taacgacttt atcttttcct ctgttagctc
     1741 ctcttcgtga aatgatacta ataaattcct tataaaatta ttcttcaaaa gattttgcac
     1801 atttcttgat ataaactcta aatatgcctt ttcttttatt gcaactgtat aatacataca
     1861 cttgcctgtt tcttgaacat ataagaatct tttctttgat agttttgata aagtttttaa
     1921 agctactttt tctggtaaat tatgttcttc tttcatataa tcaataatct catatgaatt
     1981 ggtttttata tctaaattcc aaataaattt cataataact aactctaatt ttgataattt
     2041 ttttaaacac atagttatca ctccgcttct ttatctatat aattaaatat tacaataata
     2101 atttcacaaa atcaattcta ataatcataa cataccttaa aatctgttct taacttctac
     2161 cttcttaatc tcccaaatga tttttttata ttgtattcaa tagtataatc cttttccaaa
     2221 atataactta ccagttaaaa taatctttat ttttacttac tctcgccttt tacagaagtt
     2281 aataattttt tataagttac atcaattcca aaagctccaa gaggtgcagt tataagtata
     2341 gcgagaactg ctaacgtcaa aataagttgt ccagatgcca tccccattgc aagtggtaca
     2401 cctcctattg ccgcctgtac tgtagccttt ggaatatagc caatcataca gaacaatctt
     2461 tcctttattg tcaaatttgt cttaataagg cagaagaata ctcctaccat acggaataaa
     2521 agaactccaa atattaaaat tacagctcca attcctgctt taaatgcata acttatatct
     2581 actgttgccc ctaccaaaac aaacaagaga atttcagcac caacccatag tttcgaatac
     2641 tttatagaca atcttcttgc aactacacga tattttatct gaaggaaaat cccaatactc
     2701 ataactgcta ataatccaga aaatggcaca ataccctcta aagcagtctc taaacttatc
     2761 attaaaaatg agatacttaa aagcaacacc cctttagcag aatctctcat atgaaccttc
     2821 ttaaaaaata ctgctaaaca gattccaatc actgctccaa ctattacacc taatataatt
     2881 gatactggaa tttgtaaaaa actaatagct gatatactat ttccttgtgc caatcctgtg
     2941 aatactgtaa acatcacaat tacgaaaata tcgtctatag aagttcctgc taaaagcatc
     3001 tgtggaatac ttttttctgt accataacct tcttccatta atttaagcat tttaggtaca
     3061 ataattgcgg gagaaactgc tcctactact gcacccatga ctgctgcttc caatatagaa
     3121 acgcctagta attttggtgc taaaactatc attccaataa tttcaaaagt agctggaata
     3181 aagcacataa gtacagcagg tcttcccact ttcttcaaat cgtttatatc taaagataat
     3241 ccagcccttg ttaatataat aatcaatgca atctttctaa ggtcagaaga aatatctagt
     3301 attgaattat caatcaaatt taaaacatat ggacctaata taattcctgt aagtaacatt
     3361 cccaataagt taggtaactt tatcctctta aaaatacctc ccgaggccat tcctaataaa
     3421 aaaatcaaag ctaaacttgt caacatattc ctaccctatc ctttctctaa tattctttct
     3481 cttctaatca tacattatgt tgttttattt attgttttgt tgaaattttt tgtagtgaat
     3541 aaatagattt atccatatta aatatatatg aaatagcgca tactacaaag aaataaggta
     3601 aatagctgta tccaaaaact tctgccccta taaatacagg tgcaaaaaaa gtatttgtag
     3661 cactaccaaa gacactagca tagccaagcg ctgcaactaa ttcaattggc aaattaaaaa
     3721 atcctgctaa caatacacca agactagctc caatggaaaa caaaggtgta acctctcctc
     3781 cttgaaaacc tgctgacaaa gtcagtattg taagtataaa ctttaacagc cagtcaaatg
     3841 aataaatttc tccaccatag aaactatttt gaattaaatt tgtccctaaa cctgaatatc
     3901 ttcctttata aaataaaagg aataatacac taagacatac tccaataata gcaattctta
     3961 tcataggatt ttttaatcta ttccctattt ttctttttga caatttcaga caccatgcaa
     4021 atgctccacc aaccattcca aaaataattc ctaaaacaat aagcttccaa aatattgaca
     4081 agtcaaatac aactttagaa gaaagtgcaa aagaaaattt ctctaatcct aaagcttttg
     4141 atactgaact tgctgtaaaa gaagcagtaa acgcaggaaa aagcgattga tactccagtg
     4201 aaccagctac caaaacctcc atagcaaata gtattgctgc tattggtgtc tcaaataatc
     4261 ctgcaaaacc agctgccata ccagttacta gaaaaatact agaagcattt tttataggca
     4321 accttttacc tacccaatgt gaaaaagttg caccaatctg aacagcaact ccttctcgtc
     4381 ctgcactacc accaaataaa tgggttaacc atgtacctga tattataaat ggaaccaatc
     4441 ttaaaggaat aatttcttct tctccatgcc ctacctcaaa tattagattc attcctttac
     4501 tactttttcc tccaaattta aaatagcaat aagcaataac aactcctact actgctaaaa
     4561 atggtatcag atacattgga tatgtttctc gaatatctgt aacttttaga agaaccgttc
     4621 caaatatggt gtctataaga cctataattg caccaactgg tatacctatg agtcctaaaa
     4681 aaaatagtcc accatatgtt ttataaacgc ttttaattct tattatcatt ttaatccata
     4741 ttaaagtttt aaatttttct atcattttaa ttcctttcta tttgcatcat aagatttttt
     4801 gaaagttttg ttgcatcaac tgtagtcaat gtaatgacta tcataattgt tttttaaatt
     4861 gataggatat aggtatactc ttagcccatt cttaataaaa taattaacat ataatctact
     4921 attttttcta ttaattaaat atttcttcaa atttaaaaaa taaaaattta gtaagtaatt
     4981 cacctccatt ttatcgattt aaacaacaaa aaaagctggt ggcatctatg atatcataga
     5041 agtcatcagc tcattggcag tttaggatat tatcatccaa gggagaacct cattcccata
     5101 attattatat aattttttta t
//
LOCUS       DAFKMX010000223         5083 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFKMX010000223 DAFKMX010000000
VERSION     DAFKMX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.227"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(235..435)
                     /locus_tag="LZB08_003377"
     CDS             complement(235..435)
                     /locus_tag="LZB08_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBM7316842.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(660..836)
                     /locus_tag="LZB08_003378"
     CDS             complement(660..836)
                     /locus_tag="LZB08_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316843.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(890..979)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1159..1359)
                     /locus_tag="LZB08_003379"
     CDS             complement(1159..1359)
                     /locus_tag="LZB08_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316844.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1361..1711)
                     /locus_tag="LZB08_003380"
     CDS             complement(1361..1711)
                     /locus_tag="LZB08_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBM7316845.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1873..2062
                     /locus_tag="LZB08_003381"
                     /pseudo
     CDS             1873..2062
                     /locus_tag="LZB08_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2539..2709)
                     /locus_tag="LZB08_003382"
     CDS             complement(2539..2709)
                     /locus_tag="LZB08_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316846.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3098..3586)
                     /locus_tag="LZB08_003383"
     CDS             complement(3098..3586)
                     /locus_tag="LZB08_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBM7316847.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSITIPNDKNMIITFLSNKS
                     DN"
     gene            4390..4764
                     /locus_tag="LZB08_003384"
     CDS             4390..4764
                     /locus_tag="LZB08_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7316848.1"
                     /translation="MKEEMLIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4869..5083
                     /locus_tag="LZB08_003385"
                     /pseudo
     CDS             4869..5083
                     /locus_tag="LZB08_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2031 a          631 c          579 g         1842 t
ORIGIN      
        1 ttttgatgtg ccacaactaa aattattata ttactatata ttctaattaa aagagtactt
       61 gtaggtttaa gtacatactt tataaatata tttttgtcag atttatttta taaaaagtat
      121 ttagactaag tattattttt aagtattttt ttgttgaagt tattttttcc ttttttacaa
      181 aatgcgtaaa taaaaaaaag actcttaaaa gagtctttcc gaaaaatcta tatattataa
      241 tatagttata ttttctgctt gaggacctct agcgccttta actatatcaa agcttacttt
      301 ttgaccttct tctaatgatt taaatcctga agtttgtata gctgagaaat gagcaaatac
      361 gtcatctcca ctttccattg atataaatcc aaatcctttt tcattattaa accattttac
      421 tattccatta ttcattaaaa atactcctat taacctatat caagatatac ttgatttact
      481 tgtgtattac cacaaatact attgtatcat agattaacat aaatgtctat ttaattataa
      541 tttttagtta acatttttat ttattcttaa tatgaaaata aatttaaaca ttttatatta
      601 ccaatatatt aatgtaaatg tagataaaac taaaaagggt tttctagaaa aaaatctgtc
      661 taattatact tacttaccaa taccatcact tgatgcaagc aagattccac aactttttct
      721 ccgtactcta atataaaatc atctaactgt gtatctataa aatcacaata atcatatgaa
      781 ctaccaatat aagcagttaa catatgtgta catttatcca ttttatctct aaacataatg
      841 tatttatccc cctaaaaaac tttcatgaaa aacaaaaaga aaatatcttt ttggcaactg
      901 gctgacataa cttattttat aagcgttagt ccctatagct ttgcgtcact aaatttctct
      961 agttttgccg atttagtttt attctacaga ttaataatac aatagaatta gttattattc
     1021 aacatatcta gctgaaaaat taataaattt atatatgaac gttaaattat ctgaaaaaat
     1081 aaaaagcccc tctgtcagga gtgacttaaa aattgaattt aagtttgtat ttaaaacaca
     1141 tttttaataa tgcaaaaact aatcaaaaat aaacaattca tcaataggtg tatctactat
     1201 ttttgatata tctattgcta atttcaaaga aggattatac tttcctgctt ctaaacgcat
     1261 aatggtttct cttctaactc ctactttttc tgctaattgt tcttgagtta attcttttaa
     1321 ttgtctatat ttttttaggt tacaagtgaa tttaggcata ttatttttcc tctaaaatat
     1381 aaagttttat agcataaaca tttaaagtga ttgcatatgt aattgatagt aaaacacaca
     1441 ttaattctat attcctagac actatagtaa tacaccataa aatgaataat tctataatag
     1501 caagctgtcc tgtaaatgcc atagctgctt tttcatcttg tacatacctt tcatctgctt
     1561 tatcaccatt tatttttgct ataataaagt ttgagaaaaa agcaaaaaat ccaatatatg
     1621 taaggtcagc aacattgcca gatgaaaaat atctaagact taaaaatcca aggaaaccac
     1681 aaaaacccat aaaaaatttt ttctgaatca ttttttatcc tccttagtaa ttttaaaaga
     1741 gatatattta tatctttatg atacaaatat atctctttta tattcttttg tcaatagaaa
     1801 atcagtatta tacaatattt gtactgcacc ataattgtta gagcaaaaat ctactgcttg
     1861 agatgtttat gtatgactaa atatgatttt aatttcaaat taaaagtagc taaattttat
     1921 ttgaatagag aaggaagaga tattttaata gtaaaacaat atggagttta taaccattct
     1981 caagttgaaa gatgggttaa tatattagga gagtgtgcac taaaaataaa aaaaattttc
     2041 tatactatat ggttcaagtt agatgttata aactatatga tgataactaa atatagtgta
     2101 caataaatcg ctaagtattt tggaataaac aatagcacag ttatagcaac atgaaaataa
     2161 aaatttttac atagtggtgt tgatgccttt tctaaaatga aaggaagacc ataatgaata
     2221 aaaacaaaaa cactaaagaa aaaataaatt aacaagagaa caagaattag aatatgaaaa
     2281 taaattttta aaagatgagc ttgagttttt aaaaaagatg tgtgtctcag ggaagctaca
     2341 ttaaaaaaca gtggtatcat tattaatcaa aagaaagtta gatgtattat caaaaaatta
     2401 gaacttaaat gcatagcatt ttttcataaa tcacataaat ataactccta taaaggtata
     2461 attggaaaag taaaaaataa taaaacaaat agaagattta aaacatcaat agcatatcag
     2521 aaaataacaa atgatatgct agaatttata aaatatatct aatgataaaa atggaaaatc
     2581 aattataaaa aaggcatatc tcgattcatt ttttgttata tagtcaaata agaatactat
     2641 agatgaaaga tacgattttg catatgtatt aagtaagtat tctgatgttt ttggttgata
     2701 cacatgcaat aaagaagttg tgtttgcaga taaattagca aaactatagt ataagttgca
     2761 acaataaaga aaatgaaaat tatcatacta aagcataagg catgttacaa ttttattaga
     2821 taggacatta acatacacta aagtactttt tattataaag agtacttatt tttttgaaat
     2881 tcattaatgt ataaactatc aagaatatta ctcaacataa tttaaaaaca acttgaatag
     2941 gtatttttaa attatgtgat ataataaaaa atagaagtgt atgccaatac acttctgtag
     3001 ttataactaa gcattctatt cttgaggagt gcttttttca tttttataac caactctttt
     3061 tactttaaca ataattatca gacctatata tttattttta attgtctgat ttattagata
     3121 aaaaagttat tatcatattt ttatcatttg gtattgttat tgaactacca taatcttctt
     3181 ctaatttatc tagaccactt ttacttattt ctccatctat aattatgttt aattctaagt
     3241 attcttcact accattagat gacactaaag tgtacgaacc attataaggc atgcctaaat
     3301 tttctatttt atgtgccaaa gggtcttcat caccaatacc agagtttact gttatttttc
     3361 catctttttt agtccaccat attatgtttc cttcagtata ttttgattca tctgcatagt
     3421 agctaagaga cgcacttttt attgcactta tatcagcttc aagtttagct atctttgctt
     3481 tttctatatt tttaaataat gctggaagtg ctactattgc caatattcct attatagcaa
     3541 ttactactaa taattccact agagtaaatc cttttttatt tttcatttat ttcccctcca
     3601 tataaaatta ataatatata aatttttatt ttgttgattt ctactttcta tattttacca
     3661 tatatagaca aaaaaatcac tttctatgac aaatatacta ttattttaca atttatggaa
     3721 atgatttaaa atgacatttc ttgttagaaa agatataaga aatggattaa aataaagaaa
     3781 actacagtca tatcaatgtc aataatatta tttaaaagca gatgaaaata aaaagaggac
     3841 tcagaaaata atgtctattt aattatactt acttaccaat accataactt gatataaaca
     3901 agattatact acattttttt atgctctagt ataaaatcat ctaactgtgt atctataaaa
     3961 tcaaaataat tatatgaact atcagtataa taagtcaaaa tatgtataca tgaatacata
     4021 ttttaaaaga tatatattat tacataccta aaaaactttc ataaaaacaa aaataaaata
     4081 tgcttttagt aacttgttaa cataacttat aatttatttt ataagcatta gcctatatag
     4141 ctttgtgcta ctaaatctct ttgattctaa cgatttagtg ctattctaca actaaaataa
     4201 tacaatagag ctatttattg tttggtagca ttgtttgtaa attaaacaaa tttatacata
     4261 tgaatattat tttacattca aaaaaacaga ctaaatatgt agaatatttg gtaaaactta
     4321 cctctagttt ttgtcttaaa aagctcctat tattaactta agttagaaga attaaggaag
     4381 tgacataaat tgaaagaaga aatgttaata gaaaaattat taagagcaga attaatagta
     4441 atggagtatt tatggaaaaa agacactcta atgcctaaaa aagaaattgt aaaagaaata
     4501 aaacagattt atggatggca taaaagtaca ataaaaatac tactaaaaag gctagttgct
     4561 aaaaggtatt tggctagata tattataaat tctcaatctc attataaaat aatagatagt
     4621 aaagaatatt atacttttaa gaaaaaggta ctaaaatcta gtaaaagcat aaaaataatg
     4681 cgttcactta ccactacaca taaaaatgta agtaaagaga agttagacct attagatgag
     4741 tattatagaa acttaaaaga atgatatagt aaaaatcgaa tgtaattatt taccttaatt
     4801 cttttgtttg taaaacttta ttgatacaga atttaacata tacataaatt ggtaaaggaa
     4861 tgtatactat gaaaattagt aaattaccag aagcggaatt aaaagttatg agatatattt
     4921 gggaatcaga aaaggtatta atatcaagag aaatcaccaa gtctatggaa caaaaatatg
     4981 aatggaaaga tacaactata tttacagtat taaaaagatt actaaagaag agagttttta
     5041 gaagcggaga aaatagataa gtgtacacac tataatgtat tag
//
LOCUS       DAFKMX010000224         5065 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFKMX010000224 DAFKMX010000000
VERSION     DAFKMX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.228"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            52..690
                     /gene="recX"
                     /locus_tag="LZB08_003386"
     CDS             52..690
                     /gene="recX"
                     /locus_tag="LZB08_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBM7316849.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            843..2846
                     /locus_tag="LZB08_003387"
     CDS             843..2846
                     /locus_tag="LZB08_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBM7316850.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLSFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            3020..3661
                     /locus_tag="LZB08_003388"
     CDS             3020..3661
                     /locus_tag="LZB08_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316851.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            3754..4437
                     /locus_tag="LZB08_003389"
     CDS             3754..4437
                     /locus_tag="LZB08_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316852.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(4614..4925)
                     /locus_tag="LZB08_003390"
     CDS             complement(4614..4925)
                     /locus_tag="LZB08_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBM7316853.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
BASE COUNT         1943 a          653 c          787 g         1682 t
ORIGIN      
        1 attttattag ttgcttttgt taatataatt acatataaaa aaggaggact tatgggtata
       61 attacaaaaa tagaacaaca aaaaagaaat gatgatagag ttaatattta tgtagatgat
      121 aaatttttta ttgctatatt taaagaactt gtttatacat ttaatttaaa aaaagggaat
      181 gaaataaatg aagaagaatt aaaacctatt ttagatgatg aaatgtatat aaaagctaaa
      241 aataaagcat taaatatttt atctcatgct gaccaatctg aaaaaaaatt aaaagaaaaa
      301 ctatcatcag aatttgatga aaatataatt gagagggtca tagattttct taaaagttat
      361 aatctcgtaa atgacagtgt attagctcaa aaaattgtta acacaaatgt taacctaaat
      421 aaatgtggca aaaacagaat taagcagaat ttatataata aaggtatcaa tagaagtaca
      481 atcaatgaag cagtatctga attagacaag gacactgaat ttgaaaatgc tatgtatcta
      541 gcaaaaaaaa ggtatgaaag agtaaagaaa gaagataaaa agaaaatata tcaaaaaatt
      601 tctcaacatc tttcctataa aggctttgat tatgatatta ttaaaagagt tttaaacaaa
      661 cttttaaatt ttgatgaata cgatatataa gtataaatac tagggaggta aattatgaaa
      721 ttaatcattg gtggcttaag ctttttatta ggattatata atatatatca attatacaga
      781 gaagatagta tgagataaat attatttttt agatgtaata tttattatgg aggagaaata
      841 caatgaaaag tcaaaaaaga tttaaaattt tagatattga tgtataccta caaccatttg
      901 aatctgatat aaaaaagcga atgaaacatt atgaggatat gaaatcaaaa acattgaaaa
      961 actataaaga ttttagttct tttgcaaatg ctcatcttta ctatggattt catcagacaa
     1021 atgatggatt cttctatagg gaatgggctc caaatgcaga tagtttgtca ctaattggtg
     1081 actttaataa ttggaataga aaatctcatc ctctaagaaa aattgggtct ggtaattggg
     1141 aaatatttat tcctggtcaa aatagcttac ctcacaaatc agaggtaaag gtacaagtta
     1201 ctgcaaatgg aaaaacattt gaccgtatac cattatatat aaaaagagta attcaaaaag
     1261 attttggtgg atttaatgga caaatatggc aaccaaaaac tccatttatc tggactgaca
     1321 ataattttga cttaaaaaac ataacttctc cactaatcta tgaatgtcat ataggtatgt
     1381 ctactgaaag cgaatctatt ggaacttata atgaatttac tgaaaaaata cttcctaaga
     1441 taaaaaaagc tggatacaat accattcagc ttatggctat tatggaacat ccatattatg
     1501 catcttttgg ataccaagtt tcaaactttt atgcaatctc atctaggttt ggtactcctg
     1561 aagacttaaa aaacctcata aacactgctc attcaatggg gattgctgtt ttattggatt
     1621 tagttcattc tcatgctgta aagaatactt tagaaggaat aaatgagttt gatggaagtg
     1681 aacatcaatt tttccactca ggttcaaagg gaaatcatcc tgcttggggt acaaaacttt
     1741 ttaactatgg taaacctgaa gtaatacatt ttttgctttc aaatataaaa ttttggttaa
     1801 atgagtacca ttttgatggc tttaggtttg atggtgtaac ttcaatgtta tatcataacc
     1861 atggtcttgg agtttcattt gatagttatg aaaaatattt tagtatgaat acagatattg
     1921 aagctataac atatctccaa tttgcaaatg aattaataaa agaaattaaa cctaattcga
     1981 taagtattgc agaagatatg agtggaatgc ctggtatgtg tatccctata aaagacggcg
     2041 gaattggatt tgactataga ctcgcaatgg gagtacctga tttttggata aaaactatct
     2101 ctaacttatc tgatgaagac tgggatttgg gcaaaatgtg gtatgaactt actactagac
     2161 gacctggaga aaaaaatata ggatattgtg aatcccatga ccaagcattg gttggcgata
     2221 aaacaattat tttttggcta gctgacaagg aaatgtactg gaatatggaa ataaactcaa
     2281 ataaccatgt tatcaataga gcaatatctc ttattaaatt aattaaatta ataacttttt
     2341 ctctagcagg tgaaggatat ctaaatttta tgggaaatga atttggacat cctgagtgga
     2401 ttgattttcc acgagaaggc aacaattgga gctataagta tgccagaaga caatggagcc
     2461 tttcagaaga taacaactta aagtataaac aattattaag ttttgataaa tctatgctcg
     2521 aacttacaaa atacagtgat atttttatcc aaaatagtca tgaacttata catatggata
     2581 atgataaaaa gattttggtg tacagtaagg gcaaatattt atttatattt aatttccacc
     2641 ctaatctaat acaatcagtt gatatttcaa agtttcaaaa tacaaactac aaaacgatat
     2701 taaatactga tatggtagag tttggaggaa acacaaaaaa agaaaactta agagattatg
     2761 atgtatactt tagctcaact cctccttctt ctaaaatgcc tattgcctct cgtacagcaa
     2821 tagtgctttt agataatggt atttaaatat gagccaaata tattaggaaa ctgcaaatat
     2881 agtccagtat aaggcaaaaa aataaaataa tgaaatcatg gcaatatacc tgttcaacat
     2941 gctaaaaaga cacattgaca aatcattatg tatttgctaa gataaacata ccaaatgatt
     3001 gttttgagga gtgagttttt tgggaagaaa aaaagaacct gataatacta tacaaaaaat
     3061 acttgatatt tcacataagt tgttcttaga aaaagggtat gagcagacaa cgattcaaga
     3121 tattgtagat aatttagatg gcctttcaag aggtgctatt tatcatcact ttaaatcaaa
     3181 agacgaaata attaaggcaa tcacaagtcg tttgtataat gatggatttt atggatatga
     3241 tgtaaaaaat gatgaaggaa agaccgcatt agaaaaatta caatataggt tgcttcatgc
     3301 ttatgaacaa cacaataaac aagacatttt tttaatgtca tcatcactat tgcgtaatcc
     3361 taagtattta gctacgcagt ttgatgagat tataaatgat attgtgcctt ggataaaagc
     3421 attaattgaa gaaggaaatc aagataattc tatgcaagtt aaaaatccag agattgctgc
     3481 tcaaatgttt ggttttcttc taaacacatg gtgtattcca acagtttatc caggtaatcc
     3541 acaacagatg atagaacgtt ttactttgtt tggtgagcaa gctgctttta tgggtatttc
     3601 ttttatgaat gataagataa tgtgtgaata tcaaaaattt gttaataaat tttctaaata
     3661 gtaaatataa aactgcctta atatcggcag ttttatattt acacaaaaca aacatttagt
     3721 tggtattttt aaaaatttaa tttatttcag tacatgcaaa aggaggttgc tatgaacaaa
     3781 ctaattaaag attgtgtaaa cttttttaat gattgtggct tttcatattc aatatgtggt
     3841 ggatatgccc tagaattatt tgcgaatatg catattcgcc cacacagtga tattgacata
     3901 tggatttctg acacagataa agaaaagagc attcagtata tgcttaatca aggatgggat
     3961 atatatgagc ccttgggtaa ttatttatta cgacctatta tcgaccttaa caagcaacat
     4021 atagagcaaa tttgtgtttt tgcgattaag cctgattgct catttattga attaaaaaaa
     4081 gtcgaggatg ataactataa aatgaatata cagtgtgacg aacagttaca gttcgatttt
     4141 attgaattga tatataatcc acagaaagat ggaaaatttc ttttttccca aaatcaaaga
     4201 attataagag atatggataa agccagactg ataacatcag ataatatccc atatatggct
     4261 cccgaagtgg ttttgttttg caaatcaata tacatagata ggcttggtta tcaaaaagac
     4321 ttcgatgtaa cagtcccact attatcacaa gagcaaaggg attggcttgt aaatgcttta
     4381 ataacagcat atccagatgg acatgcatgg attgaccaat tggcaaatca gtattaagcc
     4441 ttttagctcc cgaatataac attatgataa tccacactct aacagtttgt tggtttttct
     4501 agtatataac actcatatta aaataaacac taaaagccaa ataacaattt aaatacaaaa
     4561 tgttatttgg ctaatttagt ttgatactat ttaactatct tttacattca gttctataat
     4621 ttccaacttt taatttgtgg tattatattc atcttaacag tttgattctt taaagctttt
     4681 ctaaatttct cagttgaaga atctttaaac attttactag aagcactctt gaggtcttct
     4741 atagattccc aatgttgtat cataatccac ttttgagact tttcatcata taaaagctca
     4801 gtatcaataa aaccagattg aatagaatga aattttaatt ctaagtcatt tacaatttct
     4861 ataaactctt cttttgataa attttcttct acattaaact caactatttc tgtaataatt
     4921 ttcatatgta aaacctccaa tttattttcc tatatcatat caaaaaataa attggaggta
     4981 ttgtaaaaaa tcgacagtat aattataaat agctgtactt tatattttct ttaatcgtct
     5041 tattctcacc aataaattta attgg
//
LOCUS       DAFKMX010000225         5019 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFKMX010000225 DAFKMX010000000
VERSION     DAFKMX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.229"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            346..1182
                     /locus_tag="LZB08_003391"
     CDS             346..1182
                     /locus_tag="LZB08_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316854.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            1266..3380
                     /locus_tag="LZB08_003392"
     CDS             1266..3380
                     /locus_tag="LZB08_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBM7316855.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSRFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            3921..4874
                     /gene="blaCDD"
                     /locus_tag="LZB08_003393"
     CDS             3921..4874
                     /gene="blaCDD"
                     /locus_tag="LZB08_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBM7316856.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGYTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         2055 a          535 c          815 g         1614 t
ORIGIN      
        1 ataacaggac cccattaaac tttaagatag ggtaagtttg taatcatatt aaataattgt
       61 aaaaagctgt ttttagtcgt ataatataaa tatttatcat ttaattaagg aatctaaaag
      121 gaatctaata agaagaaaat aataaataac tttttagatt tactaaatga aaagttattg
      181 aaaattaaag gattagagtc tttaatcaga aagtaaaaag taatatatta aatttatgaa
      241 ttgaaaactt aaacaacaaa tttgtttata aaatagcaag attcattaaa tataactaag
      301 taataattga tataatgttg catggataaa aggagggaag ctgcaatgtc ttacaaagta
      361 attaatgtca taactgctat tgactatata gaggagcatt tatcagaaaa gctggattta
      421 gatatagttg ctaatgctgt tcattactca aaatatcacc tgcatagaac tttcacaact
      481 tctgttggcc taacaatgca tgattacata aagcggagaa agttgacaga agcagcaaaa
      541 ttattggtat tttcaaagaa accaattatt gaaattgcat tgatagctgg atatgaaagt
      601 caacaggcat tcacttctat atttaaagct atgtataaaa aatctccaaa taaatatagg
      661 aaagaacaag aattttatcc tttgcagcta agatttgttt tgaaaaaaga taacctattt
      721 tcaaagaatg tactagagtt ggaaaaagaa attaaacttg caagtatgtc tgacatacca
      781 ttatggatga atttagtgag attagtcata gatggatttc caaatttaca agaggaagaa
      841 tatatatatc aattagaaca gtatatttta gaagaaagag ctttaatatt aaaacttaat
      901 aatgtcgcta ttgcaaatat ggtgtttaat agagaaacta gaagtattga tttttttgga
      961 atacatccac agtatcgtaa ttcagacatt gcacaagtgt ttttgaagaa agttattgaa
     1021 gatttcttga tagatacaga tatttcaatt actacctttc gagagggtga caaagctgat
     1081 acaggacata gagatatgat aaagaagttg ggctttgcag aagcagagtt gcttgtggaa
     1141 tttggatatc ctactcaaaa atttattctt cctttaagtt aaatagcaag aatgattaaa
     1201 aactccaaaa cacaattaag tataatgatt cagtgtaagt acttaattaa caaaggagga
     1261 tttaaatgtc tgaaaataaa caagtagtaa attgcgatat tattgcatct aattctaaac
     1321 ataatatgtg tgaatatgat gcagagttat taactttttt tccagatgag attgcacatc
     1381 taatagagat aaataacaag ctagacaatg cttttaagaa agctgaaaat cttgttgaca
     1441 agcttgataa ggattatatg gacgctaaaa tgtatatggt aaaaaatcgt ggtgaaatag
     1501 acccacatga aatgtttcaa aatgaacaag gactaaaaca aatagataac tatggggctt
     1561 ttatggtaaa agttcgagat aagattaata aaatcaaaga ttctccatac tttgctagaa
     1621 tagattttag gctgaaagat atggatgatg aatctaagta ttatattggt agatttgcat
     1681 ttgattatga agatgagctt ataatacttg actggcgttc tccaattgcc agtatgtttt
     1741 atgattatga aatcggaaaa gctggatatg atgctccaat aggatgggtg gatggagaaa
     1801 taacaagaaa acgacaattt aaaattaaaa atggaaaatt agaatatgca ctagaaagtt
     1861 ccattaatat acaagatgat attcttcaaa aagaacttag tcacacttca gatgaaaaaa
     1921 tgaagtcaat catttcaaca atacaaaagg aacaaaatca aattattaga aatgataaag
     1981 ctgatacact gattattcaa ggggtagctg gttctgggaa aacatctata gcattacaca
     2041 gaattgcttt tcttctttat cgatttaaag ataaaatatc agcaaataat gtaattatcc
     2101 tatcacctaa taaagtcttt ggagactata tttccaatgt ccttcctgag cttggagaag
     2161 agccattatg tgaattaagt tttgaaaata tagcagaggt acaattagat agagttatta
     2221 attttgaaag tgagaaagac ccattagaaa taaatgatgc taaatggtct gaaagagtgc
     2281 gttttaaatc tacacttgat tttgtaaaac ttattgatga ttatataaaa caaatgccaa
     2341 ataagatttt tattccaaaa gactacactt ttggaagttt tacagctaaa agtgactgga
     2401 tacagagtag atttgaggca tataacagat atccagttaa gaagagatta gaaaaggtag
     2461 ctgaagacat tcattataaa tttgaatcag ataatattat ggaagaagat ttaccaaagg
     2521 taaaaagtat acttaaaagt ttgaatggaa tgttgacaat aaaaaatact cttacactat
     2581 ataaggattt ctttaaacaa atgaatatat caaacatgtt tgtgatggca gaaaagaaaa
     2641 cacttgaatg gtcagatgta tacccattta tatatattca tgctgcatat gaaggtatac
     2701 aagaagataa gattataagg catattgtta ttgatgaaat gcaggactat acacctattc
     2761 aatatgctgt aataaactta ctttttaagt gtaaaaagac tattcttgga gattttggac
     2821 aattggttaa tcctaatcat actcatacac tagatgatat gaaacagcta tataatgatg
     2881 gggaactagt tatgttgaat aagagttacc gttctacttt tgagattatt aatttcgcca
     2941 aaaagattca agacatatct tcattagagc ctattgaacg acatggtgaa gaaccagctt
     3001 tattaaaatg taataacgag caagatgaaa taaataagat aaaaatagaa attgaagaat
     3061 ttaaaaagag tgataatgcg actcttggta ttatattaaa gacagataat gacgctaaag
     3121 ctgtatatga tgcgttaaga gaagaatata gtgttcactt aatatcttct gaaagttcta
     3181 gatttacaaa aggagtatcc ataacttcta ttaaaatgtc aaaaggttta gagtttgatg
     3241 aagttattgt accttcagta aataataaaa catattatag tgattatgac cgcagtttgt
     3301 tatatattgc ctgtacacga gctatgcata aacttaagtt gacatatact ggtgaattga
     3361 ctcaacttat agatatgtag attatatact tattatgtaa gtgtttactt atataaatat
     3421 aacctgtcaa taaaattcat taataaatca ataaaaacaa tatttgtaaa agtaaaagct
     3481 ataaattata tattaaattt tataatttat agcttttata atactgctta tcttaatcct
     3541 cccaatagca aaataatttt aaacatgcca tattttataa atgtaataac aattatacaa
     3601 ttaaaataac tttcccaaac taacttgaca tttaaaactt actattgtaa tatttaaata
     3661 ttacaatagt aagttttaaa tgtggaatca attattatat cacaaattaa aaggaggaca
     3721 ggatgttatt aatagttacc taattatctt cgcaataggt ttaatcaaca taattgtact
     3781 aggtgaagaa aatataagag atacagctat tcatttagtc ttgtttctta taagtagaac
     3841 ctagattgga gtatattaca aataatgtct actattttaa tagtaggtta taaatttaaa
     3901 tatgggagat gatataaatt atgaaaagaa aaaagaattt tatttggata gctatcttat
     3961 tggttggtgt agttatggta atgtattatg tagggaaaaa acataacgat ataaatcaaa
     4021 aaactgataa gaattatata aaatctgaat taaataaatc tgaattaaat aaatccaaaa
     4081 ttgaaaacaa taataaaaac aaaaaagaaa aaattaatat ggttgactat agtgattgtt
     4141 ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa tataatatat
     4201 ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa atagtttcaa
     4261 ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg ggatatgatg
     4321 gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag gcatttaagg
     4381 agtcttgtgt ttggtactac aaaaaattga taaataaagt tgatgctaaa tctgtccaaa
     4441 acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa ggagatttaa
     4501 aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa atatctccaa
     4561 aagaacaagt gcaaacaatg gcaaaaatat ttgagggata tacaaacttt aaaaaagagc
     4621 atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac ataaatgtat
     4681 atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca tggtttgttg
     4741 gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat gattccaata
     4801 aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag aaatattata
     4861 gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatact ctgtaatatt
     4921 tattaaataa ttttactaaa ttgaatctat atagagcaaa actcctttag tacataaaat
     4981 acttgctatt gattattaaa ttgaatacct atagattta
//
LOCUS       DAFKMX010000226         5007 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFKMX010000226 DAFKMX010000000
VERSION     DAFKMX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.230"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            298..2805
                     /locus_tag="LZB08_003394"
     CDS             298..2805
                     /locus_tag="LZB08_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7316857.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            3147..>5007
                     /locus_tag="LZB08_003395"
     CDS             3147..>5007
                     /locus_tag="LZB08_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316858.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIII"
BASE COUNT         2037 a          488 c          815 g         1667 t
ORIGIN      
        1 ggcgattttt agtaaatatg agaaaataat gtgtttttag gggaaaagtt aaatacagat
       61 ttaagtgaac aagaatcgat aattaagata ttaaatggtg aatagggcat caaaaaaatt
      121 actaaattaa aagttttaga gggagaatct tagcaagtgt gtgtgaaaat tcaatttcaa
      181 acaaaaaata attgtaaatg aaatcaattt atacacgttg aagattcact tttatcatta
      241 tattaaaaag cagagtgaat taaaagtgag tctaaagata ggaggataat taaaacaatg
      301 aaagaagaca ttaggttaca tcgaatgtgt aaaaaagaac aagcaacaaa gaatgaagtt
      361 atatcagagg atattctaaa atctatgagt attggggtca gtaaacattt tttagatgat
      421 tcatttacaa taatatgggg aaatacatca ttctatcaat tattaggata tacagaagaa
      481 gaatttttag ttaaattttc tagtttaaaa gattattatg aacagaatct ttatcatttt
      541 gaatctatga aaaatcattt tttagaggct tatcatcatg gcgaaaagag tatagaatat
      601 caaatttgca tttcagcaaa taatggagac cctgtttggg taataatgac tggtatatta
      661 attgaatcac aagataatga gaaatcaatg gtctatatat tgtattcaaa aattaatgag
      721 ttaaaaataa agcaagaaga actatctgat atggaaaaag agagtacaga gaattttaaa
      781 tggatgatgt cagagtatgc tgggaatgtt tatattagcg atatggatac atatgaatta
      841 ctatacttaa ataagcactc atgcaatact ttacaggcat cagcaaatga acttattgga
      901 agaaaatgtt atgaggtaat tcaaggtaga acttcgccat gtccgttttg tacaaattcg
      961 tatttaaaaa aagatgacat atatgaatgg gaattttata ataaaaaact gaaacgaaca
     1021 tttataatta aagaccgtat gttgaattgg caagggcgaa gagttcgtat tgaattatct
     1081 tatgatatgt atagtgctga atataaatta gcaaaaaaag atcaggaaag agaatcaatt
     1141 ttgaaaacta ttccagcagg aatggttaga ttagatgcaa gagattatct tactatactt
     1201 tggtgtaatg atatattttt gaatatgatt gaatatacaa aggaacagtt tgcagaggaa
     1261 cttaataatc aatgtggata tttattttct gaagattata aacgtagtaa gaaactggca
     1321 caagatttga aggagtctgg ggataatgtt gtatttgaag caaaaatata tacacgttca
     1381 agggaagaac gtatatggac tgttacattg tgttatatca gtggggaaga cagttgggat
     1441 ggaataccat ctttttatag tataggtatt gacataacaa aggaaagaaa acagattgag
     1501 aaattacagc atatatcaga gaaagatgca ttaacaggaa tttataatcg tgctgagact
     1561 gaaaggcaga ttaagaaata ttttgaaaaa aatttaaatg ttatgggtgc actgtttatg
     1621 attgatacag ataattttaa acagattaat gatacagaag ggcatatgat tggagatata
     1681 gtacttactg agatggcatc aggtatgaaa aaaataatgc gtgacagtga tgttgttgga
     1741 agaataggtg gagatgagtt tacaattttt atgaagaata tttcctcagt taaagatgca
     1801 gaaaagaaag cagaagaact attgtatatg ttccgtcatc tatttcagaa agaaaaaagt
     1861 tttctaaaag taacttgtag cataggaata gctatatatc caaaggatgg tactacattc
     1921 aaggaaatat atgcaagagc tgataaagcc ctgtatcaag caaaaaatat gggaaaaaat
     1981 aattatgtaa tctacaacca agattatttt aaagaattag aagaatttga ttgttcttct
     2041 cttggtacag ttattgattc agaaaaacaa tacgctgaat atcctgataa tcttacacgt
     2101 tatatattcc gtatgcttta tcagactgat gatatagacc aggcgattaa tatgatactt
     2161 gaggttgttg ggaaacagtt tgatgtaagt cgtgtttata tttttgaaaa tacagaagat
     2221 ggtagatata cttctaatac ttatgaatgg tgtaatgaag gaatctcttc tcaaatggct
     2281 tatttgcaaa acagcaatta tcaagactat gaggattatg aaaagctttt tgaggatgaa
     2341 ttagtttttt attgtcgaga tatccataca ttaagtccta aactagaaga attgttttct
     2401 agtcaaggta tccactctac acttcaatgt gcatataaag aagatagagt tttcagtgga
     2461 tttgttggat ttgatgaatg cacaggacta cgtctatggt cacaagagga aataagtaca
     2521 ttatcattga tttctcaaat tatatcaatg tttttacaac gtaaaaaaaa taataaactt
     2581 aatcaacaga ttcaacaata ccagaatatt ttagataata ttgaccagtg tatttgtgtg
     2641 attaataaag aaaataattt tttattatat gtaaataaaa aatttaaaaa tacatattct
     2701 aaattccaaa ttggacaacc ttattatctt gattctatag atattcataa gatatccatt
     2761 atatggaata aaaaagaagc atacctatgt actagcacga tttaatggta aagtaaagtt
     2821 tatattggac ttaaattaaa tttcgtttta aataagatat tattttagtt gcttactgaa
     2881 aagactagaa tctatattaa tgaattatac taattttata ccactattaa aatattgttt
     2941 gctaaaaata aaaaatagcc ttaaaacagt tgattataaa aaattaactg ttttaaggct
     3001 atttttatct ttgtgacgaa atagacactt gccatttaga atgtgacaat atagtagttg
     3061 tttagaaaag gcacaaagca ttatacttta actaaagtaa aatgtatttt gcttttagaa
     3121 taaaaacaat taggagggag ctataattga aaaagataat atatgtatta tttacaattg
     3181 aactattaat agtatcttta ttaggtctaa atatagttaa agataatgaa attaataata
     3241 ttttatataa cgatacaaca agtatatctg taatgtttaa agattataaa aagttaaata
     3301 aaaattatag taagtggatt aaagatattg cagatgaaaa taatgtggct atatcaaagt
     3361 atgtttttaa taataatgaa aaattatcta tatatacgac tgatacaagt ttgaataaca
     3421 atataaagtt aaaatcagga aaatttccta atactaattc aaatgaattt attagtaata
     3481 taaacactaa taatagtaat caagtaggta aattctcaac tatgagtaaa gacatatcaa
     3541 ttttaattag ggattttggt aaaataagtg aagttggaga atcaggaatc ttatatattt
     3601 caactcaaaa tgaagataca ataaataaaa tattaagaga actaaatgta gacagaagta
     3661 tttttgtagc aagattacat gatagatatg ttaactcaaa tttatactta aatccttcaa
     3721 taattagaga tttaatacta attatccttt gtttcttggc tacaataata cattattcaa
     3781 ttagtgtgag tagagaggct tctattttaa gattaaatgg atactctaag ctagatataa
     3841 ttgctagagt tataaaaaat atacttagaa ttatgatttt atcatctgtc acagcattat
     3901 taatatatat aatctatatt ttaaaattaa atataggtgt tagagcatgc ttatactttg
     3961 ctatgttttc gattatatgt attttgataa atattttgat atctatatta atagttggtt
     4021 ttaatagtag aagttctaaa tatgtgctat ctctaaatgg aaaaaaatct tatggttttg
     4081 taaatataat gcatttcaca ttgaaaattg tatttgtatc atttcttata ttatccataa
     4141 ataactgtat tttaaattat aatatgctac aaactcagtt aggaaatcta tctgagtggg
     4201 ataaagcaaa aaatgtttat aatcttactc ttaaagatac aggcgaacaa tctttaggaa
     4261 aggaagaagt aattaaaaat gccaagatta aaggtttcta taaaaatttg gtagaagaaa
     4321 aagatgcttt cttaatagat tcaactaatt ttgaaaaact tgaagatgga agttacatgt
     4381 atgaaataaa ttcaaagggt aaaaacccag aagtaagtca atatggtaaa aatataaaaa
     4441 taaataaaaa ctatcttaaa gttaacccta tttactcaaa tggcaaagaa atatttaatc
     4501 taatcgatta tgacgataag actataaatt tattagttcc taaaaagtta caagtacatg
     4561 aaaatgagat taaaaaagag tttagagaat tattttattt tgaaaaaata gaagtagaaa
     4621 atatgtacaa taaagagtta aatagagagt taaataaaac aaagaagtca gatttaaatg
     4681 ttaatataat atatgtagat aataatcaaa gctattttac ttataactcc cttgttatgg
     4741 atgacaatag gaatctcatt gatgacccaa tagcaatagt tgacatagat aatattgatg
     4801 attcttttta tctttcttat atatctagat gtgtatattt taattctaag aaattagatg
     4861 cccttgcaga tataagtaac attattgaag ctcaaggtgt agaagcgcat atccaaagtt
     4921 tacatgctgt ttataatgag tatggtttag aaataaataa actagaaaaa tatttgaata
     4981 gtgaaatttt tactataata ataatag
//
LOCUS       DAFKMX010000227         4992 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFKMX010000227 DAFKMX010000000
VERSION     DAFKMX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.231"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            204..578
                     /locus_tag="LZB08_003396"
     CDS             204..578
                     /locus_tag="LZB08_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7316859.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            580..1437
                     /locus_tag="LZB08_003397"
     CDS             580..1437
                     /locus_tag="LZB08_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316860.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            1440..2078
                     /locus_tag="LZB08_003398"
     CDS             1440..2078
                     /locus_tag="LZB08_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBM7316861.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3089..3823
                     /locus_tag="LZB08_003399"
     CDS             3089..3823
                     /locus_tag="LZB08_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7316862.1"
                     /translation="MNLAKGLDVLKISSNVLGEDKVMYIPAIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIETLKNLIIE"
BASE COUNT         2008 a          508 c          754 g         1722 t
ORIGIN      
        1 tacttttaat aaatatttaa tacatcctat cttatgtagt tttaaaaagt gtttatggct
       61 attaacaatc tctaatattt cattcatatt tattttgtca tagtaatatt ttacatcttt
      121 tatattgaca aaaaagtgct ctaagtgtat agtgtgtata tgataacatc acactgaaag
      181 tgataaaaag ggggtgataa aaaatgaaag tgctcatttc taacacatca gataagccat
      241 tataccaaca aattaaagag caaattaaag aagcaatatt tactgaagaa ctgaaagaag
      301 gggatgcaat gccatccata agaaattttg ctaatgattt aaaagttagt attttgacta
      361 ttagacgagt gtatgaagaa cttgaaaatg aaggctttct tacacgccaa gttggaatag
      421 gaacatttat ttcaactgga aatttagaaa tactaagaga ctcaaagaga cgtatagttg
      481 aacaaaaaat ggcagatatg attaaagatg caaaagcact aggaattaca cagatagagt
      541 tacaagaaat gatggatatt ctatatgagg aggattaata tggaaaatat tttagaagtt
      601 tataacttaa ataagactta taccgatttt tcattatcta atataagctt tgcattacca
      661 gaagattgta taaccggatt tataggtgtt aatggagcag gaaaaacaac aacaatccgt
      721 tcgatattgg atgtcgtaaa gcgtgatagt ggaaaaataa aaatctttgg taaagatttc
      781 gatggaaatg agaaggaatt aaaaaatcga attggagttg tctttgatga ttcatatttc
      841 tatgaggagt tgtcaatggc agaaatgaaa agtattattg caccttcata tgataattgg
      901 tgtgaagaag attatcaaca aaatatgaag cgttttaatc ttaacccaaa acaaaagatt
      961 gatacacttt caaaaggaat gaaaatgaaa tttgctttag cattagcatt atcgcataaa
     1021 gctgagtttt taattatgga tgagcctaca agtggtttag acccactaat ccgtagtcag
     1081 atacttgaca tactaacagg atatatgaag aaatgtggtc gtggtgtttt cttttccact
     1141 catataacat ctgatttaga caaaatagca gatatgattg ttatgattga caatggacgt
     1201 attatattag agcgtgaaaa agatactcta ttggacgaac atagaaaagt aaaaggggat
     1261 attcaatttc taaatgatga aaccaaaaaa ttatttttaa atttagaaat tacaggttat
     1321 ggttttatag ggattacctc aaaaattaat gaagtgcaaa aaaatatgcc agatatttta
     1381 atagagcgtc ctacaattga agatattatg cttgcatata ttgaaaggag agagtaatta
     1441 tgttgattca tttagttaaa aaagatattc tgattgtaaa aaaatatgta ttgttgatat
     1501 tatttattgc aataggaatc ccattattta tattatggcg tttacctcca tttgcacaaa
     1561 tattgggatt tgtactatca attatatttt cagaatttat gctgtgtcag tatctattat
     1621 taaaggagag tcaatattca aaagcatctg tattactttg ttcaactcct tatcctagac
     1681 gtggcttagt tcaatctaag tatgttttat ttgcaatggt atttatctat tgtacattag
     1741 tttactggat tgaaaattta cttattccac aaataggaac ttttgactta acatatattt
     1801 tgattgtttt attaataagt tctattattt atggagttta tatgcctatt caatacaaat
     1861 taggatatga aacaacaaaa ttctttttcg tggtgataat catggcttca tcatttgtat
     1921 taccattaac tatagctcac agcaattttg aaattccatt tatatatcag tatccatcca
     1981 tgatagtgaa tattgtattg ttccttttaa gtttaattat actgttagct tcaatgttta
     2041 tttctattaa aatttttagt aataaggagc ttgtataagg taagggggac ttttatatgc
     2101 cacatttacc agattgggct gttttacttt gtatcttact tccaatagta tataaagctt
     2161 gtcagatatt taaaagtaaa aaatagagta catatttata ttgccatttt tattgagtga
     2221 agttagatgg tatgaattta aaaggttact tgaaaataaa gttacttggt gcgggagagt
     2281 aacatcataa tattatagat tatatgtttc ttgtgaatca atttgtattt tttaataggg
     2341 aatatccagt aattttgttt tccattgatt atttttcaat tatgagtatg attgtgtgta
     2401 ttgtatacca tcttttaagg tgtttttaaa aaattatatc gtcaaaatat aaatctaagg
     2461 aatcctataa ataaagcttt ttaattttag aaaactaata aaaagtagga agttaataaa
     2521 aagaagctgt ctcaaaatag agataagctt ctttttcatt gctgaaatga cgaaaatata
     2581 attgatttca tataaaaaag agtgctttat gaactattta ttttgaacat tagatacact
     2641 tgcactcaat ttagttactc ctaagcttta tgaactaaaa gtttattttg aagcttattt
     2701 ttattaaaag agattttatt tttctaaata atcatctata tacagtattg cgtttctttt
     2761 atttattcat aatttatttt cttattaaag tttttctata ttcatatttt ccatattact
     2821 ttacttttaa ttttatacga attatcaaaa ataaaatagt tattagaaaa aaataaaagt
     2881 aattgcaaag cttgcaatta cttacttcta tattaattac tataccataa aataacaaaa
     2941 aaatcaagtc gagtaatcct tcaacttaag tgatatacta ggtattaata atttttaatt
     3001 aataaaatat aactagagga aaatctacat tttctcaatt tctttaatta aattgacctc
     3061 taattaagta attataagga gaaaaattat gaatttagca aagggattag atgttttaaa
     3121 aatttcatca aatgtattag gtgaagataa agttatgtat attcctgcaa tctacacaga
     3181 agatgatgca acacttattg acacaggtct tccgggtcaa ggtgatttaa taattgatgc
     3241 tcttaataaa agtaacacga gctttgatag gttaaaaaat ataataatta ctcatcatga
     3301 tatagaccat ataggaaaca taaattatct aagagaaaaa tctaagaata atatcaaagt
     3361 atatgcatac aaaagtgaaa tcagctatat aacaggagaa gaaactcctt ttaaacttta
     3421 tatgttggaa caaatggtag ataaaattga tgataagatg cttagtatgc taaatgttat
     3481 gggacttgga tttaaatcat catatactaa agtagatgtg tcattagata accatgaaaa
     3541 attgaatcta ggtgaggaaa tagaagttat acatacagga ggtcacacta gaggtcacat
     3601 atgtctttat ttaaaagagt ctaaagtact tattgctgga gatttgttac aggtagaaaa
     3661 tggagaactt aagcctgttg atgtaatgca cagcaacaaa caagagttaa aagatgctat
     3721 taaaaatata agtaattatg atatagaaac gatagttttt agtcatggtg gattatatca
     3781 aagaaacata attgaaacat tgaaaaacct aattattgag tagtactata tgatttacaa
     3841 taatttctta aattacttaa aattatatct aaagatgtag aatcaaaatt aaatataagt
     3901 aataagaatt ttgctttaaa attatactta agtgttaagg gtgatacgta gtattacaag
     3961 tattttagaa tatagttttt tatagtaatt ttaaggttct acaatacttc agttgaaaaa
     4021 tatttaacag catagtataa tgggaaagaa aatattttaa ataaacttta gtatacaaag
     4081 tgagtaaaaa tatttaaatc atatatcata atgcagagtg aggtaaaata tttaagttat
     4141 atatcataat acagagtgaa gaaaaatatt tagataatgt accataatac aaagtgagta
     4201 aaaatattta aagcatgtat cataatgcaa agcgaagaaa aatatttaaa taatgtacca
     4261 taatgcagag tgagcaaaaa tatttaaata atgtgtcata atacaaagcg aataagatta
     4321 tttaaagcat gtgttataat acaaagcgaa taagattatt taaagcatgt gttataatac
     4381 aaagcgaata agattattta aagcatgtat tataatataa agtgagtaaa aatatttaaa
     4441 tcatgtatca taatacaaag taaagaagat tatttaaacc atatatcata atgcaaagtg
     4501 aagaaaaatg tttaaattat gtatcataat ataagcagag tgaagaaaaa tatttaaata
     4561 atatatcata atgtagagtg agtaaaaata tttaaatcat gtatcataat gcaaagtgaa
     4621 gaaaaatgtt taaattatgt attataatat aagcagagtg aagaaaaaat ttaaatcatg
     4681 catcataata taagcagagt gaagaaaaat atttaaataa tatatcataa tgtagagtga
     4741 agaaaaaatt taaatcatgt atcataatat aagcagagtg aagaaaaaat ttaaatcatg
     4801 catcataata taagtagagt gaagaaaaat atttaaataa tatatcataa tgtagagtga
     4861 agaaaaatgt ttaaattatg tatcataata taagcagagt aagtaaaaat atttaaatca
     4921 tgtatcataa tgcaaagtaa agaaaatcat ttaaattata tatcataaaa ataacatgaa
     4981 atattgaaca ct
//
LOCUS       DAFKMX010000228         4981 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFKMX010000228 DAFKMX010000000
VERSION     DAFKMX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.232"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            24..2858
                     /locus_tag="LZB08_003400"
     CDS             24..2858
                     /locus_tag="LZB08_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316863.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            2901..4145
                     /locus_tag="LZB08_003401"
     CDS             2901..4145
                     /locus_tag="LZB08_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBM7316864.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(4478..>4981)
                     /locus_tag="LZB08_003402"
     CDS             complement(4478..>4981)
                     /locus_tag="LZB08_003402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBM7316865.1"
                     /translation="IKTSSDEDVIKTDGTIVPPDAEKTVKLVFTVTHTASSKTADTAE
                     IDVVVPAKSTDEELQTAVNNEAAKITNVAEPAKDATKLTMPTVSAGYEIAIKTSSDED
                     VIKTDGTIVPPDAEKTVKLVFTVTHTASSKTADTAEIDVTVPAKTVSTPTSLLGRIAV
                     NIFNFSK"
BASE COUNT         1829 a          675 c          778 g         1699 t
ORIGIN      
        1 aacataaaga aaggaaacgg tatatgctaa gaaaagataa agttctatta tctctacata
       61 aattatgtga aaatatgact ttaaaagacc ttcaatcaga taaaacagga ttcactacaa
      121 tagaaattgc taaccattca tctcttgata gaagtaatac cagtaaagaa ttaaataacc
      181 tatacaatga aaaaaaagtt ctgaaaatat ctggtaagcc aattttattt ctacatgcta
      241 gtattataga aaatttaatt ggccgtactt tgaacagtga tgaattttct ataaataatt
      301 gtaatgagct acttaaatca agtaaaaatg actcagaaga tattttttca acattacttg
      361 gacatgattc tagtcttaaa ctacctatag aaaaagctaa atctgctata ctctatcctc
      421 caaaaggtct taatacgtta ctcataggac ctactggtgt tggaaaaagt acatttgccg
      481 aaacaatgta caaatatgct gtagaaagta aaatcttttc ttctgaaagc aaattcgtag
      541 tatttaactg tgctgagtat gcagaaaatg ctaacttatt attaagtaat ttatttggtt
      601 atgtcaaagg ttcttttact ggtgctaata aggataaatt cggcatagtt gcacaggctg
      661 atggaggcat attattctta gatgaaatac acagattacc accagaaggt caagagatgc
      721 tttttctttt gatggacaaa ggcatataca gacgattagg agaatctgac attgtgcata
      781 aagctaatgt ctttattgtg tgtgccacaa cagaagatat acattcttct ctgcttggaa
      841 cttttttaag aagaatccct gttacaatca acctacctgc tttacaggat aggtctttaa
      901 aagaaagatt atctcttatt aaatacttct ttattataga atcaaaatac accaatgcaa
      961 ctataaaagt tcacaaagat gtaattaaat cattactcct ttatgattgt tttggtaata
     1021 taggtcagct taaatctgac atacaactta tgagtgcaag agccttctta aattacaaaa
     1081 ctggacttaa ggataatata gaaatagatt tatcccttgt tcctgattac atctatattg
     1141 gtcttttaaa attgaatgaa aatcgtagta atctaaataa attatcagaa cttgattata
     1201 tgctttttta tgaatttaat ggtaataact tagaagttga atatacaaaa gaactatcta
     1261 aatacaatat agatacatca aacaatggat ttaaatactc tacagagatt ccttatctgg
     1321 aaaatgatat aaggagtatg tttataaatt acatggaaaa gtactctaca aaattacttt
     1381 cgatatcttc agtaactcat tctgattata caagtaatga gatttttaaa gtaatcaatc
     1441 caaagattta ctatgctgtt gaacattctt taaaagaggc tgaaaaaata tcgagaaaca
     1501 aatacacaaa acaaacttat attgctcttt caatgcatat aagtgcactt atagaaaata
     1561 taaatcaaga cacatataat cctaagtaca atttatataa tgataaaatt ttcttagata
     1621 aagatttaaa tatagctaaa atagttttaa gtataattga aaatgactta caaacatctt
     1681 tacctaaaga tgaagtaaaa tttatatcca tgtttctaaa tagcatgtat cttgaaccaa
     1741 acaatcaatc tgaaagtata ggagtcataa tacttgctca tggaaattca acagcaagca
     1801 gtatgtgtga tgtagcaaat tcattgcttg gtacaaacca ttgtaatgca attgacatgc
     1861 ctcttgatgt aaaagtaagt accatacttg ataaaactat agagttggta aaagaatgtg
     1921 acgaaggtaa gggtgtactt cttttaacag atatgggctc tcttatagct tttggagaac
     1981 ttatttcaga aaaaacaaaa atcaaaactc gctcaattga aatggtatct actccaatag
     2041 ttttagaagc agtaagaaaa agtacactac cagaaatgac tttggatgaa ttagctaatt
     2101 ccttaagctc tttaaatcca tatattggac gagcagcaat cgattcaaca gaaataaact
     2161 atcctgaaaa gtcaagagag tatgtaataa taactacttg tataacagga tatggttcag
     2221 ctatgaaagt agcagacttt ataaattctt cacttaaaaa tattagtaaa tacaatatta
     2281 atttagttcc tcataatata gaaagtttta aaatgtataa gaataaattt agtaatacta
     2341 aaattctggc agttgttgga agtgtagatt ttaatgtagc taacacccct tttatacaca
     2401 tagaggaact actaaatgga tatggcctag atatgctaaa caaaataata gaaggtacat
     2461 tcaatgccaa tagtattaca aatataaaag aaacaaatct atcatacaac agtttaataa
     2521 taaactttat gacccaaaac ttagagcttt tggactctgt taaattgttc aactatgtca
     2581 attcttcctt atttacaata atagaattaa tagatgaaaa cttaaagagc aaatttaagc
     2641 taggatatat actacattgc tcttttatga tagaaagatg ttttaaaaat acaacttttc
     2701 catataataa cattactgaa ttaattagta aaaatcatga actatatagt ttaatacgtg
     2761 aagcctttaa gccaacagaa gcttatttta atattttgat accagacgaa gaaattgctt
     2821 atataataga catgattaat gagtatctaa ataaataaaa tttatagcac ttattttgtg
     2881 catattaatt aatatatgaa atggaggacg agattttgaa aggaaaaata aaacagttaa
     2941 caatacttgc attaattttt atatttataa ctcctgtatt tgcatttgca gatactccac
     3001 cagtaccaaa ttcttcaaga gcagcactac ttatagacca ggaaactaaa agaatattat
     3061 ttgaaaaaaa cattgatgaa aaaatgccat tagcgagttt aagtaaaatg atgacctttt
     3121 tattggcaat agaagcagtt gataaaaatc aagttaaaga gactgatatg gtgaaaatag
     3181 acaagtctac agcttcagta ggaggttcta cttgtaaact taaagatggt gatgaaatat
     3241 ctctaggaga gcttatgcag ggtctaatgc tagtatctgg aaatgatgct gctatagcca
     3301 tagctaaaca tattggtaaa actgaaaaga attttgtaaa tatgatgaac aagaaagcag
     3361 aagaaatagg aatgattgat acgtattatt ttaatccaaa tgggcttcct atatatactg
     3421 acccagaaca taaagaacct cctattgaaa atatgtcaac tgctcacgat atagttacac
     3481 ttggaaagta tatgtacgac cattatgaaa atcaagttac aagaataacc actatgcaag
     3541 tatataatga tactaaaaag gattttacac attataacac aaatcctctg cttgtttcag
     3601 tacctggtgt tgatggaata aagacaggat atacagataa tgctggatat tgccttgcat
     3661 tttctatgat ggtacctaaa gatgctaaaa acgaaagaaa tcatagattg ataggtgttg
     3721 tacttggtga tggtaacaag aaaaatagaa tttcttcttc tgctacttta ttgaaatatg
     3781 gtaaagataa tttccattct aaaaagatag cacataaggg tgatattata gagactcctt
     3841 gtgttgatgg tattgatgat tttaagataa cagttaaggt tgataaagat ttatatggtg
     3901 ttgtttcaga taacgaaaat atcaatccta aggtagtgtt taaaaatatg aattatccta
     3961 tccataaggg agatattgtt ggtgttgcaa agtattataa tgactctggt aaatttgttg
     4021 gaagtgttga tgtaaaaagt gaaagtaata taggatgtat tcctttgaag gataagatta
     4081 agattaaggt tgctaagata aataaaaaac ttgagattaa aaattctgtt tgttttaaag
     4141 catagtaaag catattatta aaagggtatg ttaaatttat aaagactcca ctttgtggag
     4201 tctttttgtt ttttacaaaa aattaaaata gtggcaacaa ttgaggatgt gagggagagt
     4261 tgccactatt ttgtataggg tattatatta tgtttttaag ctagttttac tatcttctat
     4321 gtttatataa taactcttgt gtgtgaattt aattgataat taatatgaaa attatgtgaa
     4381 gttttatgtg aatcttatta taagcatact aaaagagctg taggtaagtt tacttttatg
     4441 caaatttaat tacagctcct ttcattatat attaatttta tttactaaaa ttaaaaatat
     4501 ttactgctat tctacctaat agagatgttg gtgttgatac tgtctttgct ggtactgtta
     4561 catctatctc tgctgtatct gctgtttttg aacttgctgt atgtgtcact gtaaatacta
     4621 gttttactgt tttttctgca tctggtggca ctattgttcc atctgtcttt attacatcct
     4681 catcacttga tgtttttatt gctatttcat accctgctga tactgttggc atagtcaact
     4741 tagttgcatc ttttgctggt tcagccacat ttgttatttt tgctgcttca ttatttactg
     4801 ctgtttgcaa ttcttcatct gttgacttag cgggtactac tacatctatc tctgctgtat
     4861 ctgctgtttt tgaacttgct gtatgtgtca ctgtaaatac tagttttact gttttttctg
     4921 catctggtgg cactattgtt ccatctgtct ttattacatc ctcatcactt gatgttttta
     4981 t
//
LOCUS       DAFKMX010000229         4972 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFKMX010000229 DAFKMX010000000
VERSION     DAFKMX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.233"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(286..2118)
                     /locus_tag="LZB08_003403"
     CDS             complement(286..2118)
                     /locus_tag="LZB08_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316866.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2111..4357)
                     /locus_tag="LZB08_003404"
     CDS             complement(2111..4357)
                     /locus_tag="LZB08_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316867.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SIYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4425..4886)
                     /locus_tag="LZB08_003405"
     CDS             complement(4425..4886)
                     /locus_tag="LZB08_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316868.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEKEFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
BASE COUNT         1564 a          929 c          561 g         1918 t
ORIGIN      
        1 attctattta taataaaatt taaagaataa aattaaatat acattttgat aatcacctcc
       61 aacattttcc ttactacata ttataataaa ataaacactt tgtaaagaca taaaattatg
      121 tggctatctc aaaatataga taatcctctt tttcattgtt aaaatgataa aaatacactt
      181 tatttcatgc acaaaagagt gccttatgaa ttaaaaagtt cattttaagg caccctcatt
      241 tattttatct tattaattac atgtctatgc taagatagtt ttttgttaca ttgcttcatc
      301 ttcttcaaat tgagaattgt aaagattggc atagaatccg cctttcttta gaagttcctc
      361 atgatttcct tgttcaacta tatctccttc attcattaca agaatcatat cagcatcacg
      421 tatagttgat aatctgtggg ctataacaaa acttgttcta ccttccataa gattatccat
      481 agctttttga atcaatactt ctgttctagt atcgacagaa cttgtagctt catctaatat
      541 taaaatttta ggatcagcta aaatagcacg agctatagtc aataattgtt tttgaccttg
      601 agaaacatta cttgcttctt catttaattt catcttatat ccatctggta atgtcttaat
      661 aaatctgtga acatgtgcag atttagcagc ctctataact tcttcatctg tagcattcaa
      721 cttaccatat cttatatttt ccataattga accactaaat aaccatgtat cttgaagaac
      781 cataccaaac atttctctaa gctcactacg attaaaatct ttaatattat gaccatcaat
      841 cagtattgaa ccgctattaa catcataaaa tctcattaac agtttaacta ttgtagtttt
      901 acctgctcct gtaggtccaa caatagcaac tttttgacca ggtttaacat ttactgagaa
      961 atcatttatt atagtcttgt ttgggttata tccaaagttg acattttcaa aatcaatttt
     1021 accttctaaa ccatctattg aaactgcatt ttcaacaatt tggacttctt cttcctcttc
     1081 taaaaactca aatactcttt cagaagctgc tgctgttgat tgcaataaat tggcaacctg
     1141 tgcaatctga gtcattggct gtgtaaagtt tcttacatat tgtataaatg actgtatgtc
     1201 accaacctca attgttttct taattgcaag ccaacctcct aagattgata ccattacata
     1261 tcccaagttt ccaataaaca tcataagtgg ttgcattata cctgataaaa attgagattt
     1321 ccacgctgaa ttatacaggc tatcatttag tttatcaaac tcttcaatta cttcatcctc
     1381 acgattaaat gcttttacta ttgtttggcc actgtaagtt tcctcaactt gaccatttac
     1441 atttcctaaa tattcttgtt gatatctaaa gtatttttgt gatttcttta ttattcttga
     1501 aattacaaac attgatattg gtaatattaa aactgccact aatgtcataa ttccacttat
     1561 agataacatc attatcaaaa cacctatcat agttgttact gatgttataa gctgtgtcat
     1621 actttgattt aaactttgat ttaaagtatc tatatcattc gtaattctcg ataatatttc
     1681 accatgcgtc ttagtatcaa aatacttcat tggcaatcta tccaattttg ctgaaatctc
     1741 ttttcttaaa ttataggata ctttttgaga aattccactc attataaatc cttgtataaa
     1801 agagaacagt gcacttatca aataaaggaa aagtaaaaag gttaagattt ttccaattgc
     1861 attaaaatct atacctacgt ttcctccaga aactttactc actaaccctt caaatatctt
     1921 tgtagttgcc tttcctaaaa tttttggacc tactatagaa aacgatgcac taccaatagc
     1981 aaatacaatt actaaaatta ttgatatttt aaatttagat aaatatgcaa ttagttttcg
     2041 catagtccct ttaaagtcat ttgctttttg tcctgctccc atagattttc ccataggacc
     2101 tcttcccatt ctactcatta gctagctcct cctttgaaag ttgtgataaa gctatttgct
     2161 cataaacttc gcaatttttt agaagctctt tatgtgttcc tattccaact atttcgccct
     2221 catttaaaac aattatctgt tctgcattta agatagtact aattctttgt gcaactatta
     2281 gaactgtact ttcagcagtt tttgatttta aagccttacg aagagctgca tcagttttta
     2341 agtcaagtgc tgaaaaacta tcatcaaata tataaacttc tggatttttt gctattgctc
     2401 ttgcaataga aagtctttgt ttttgacctc cagatacatt tgttccacct tgagaaattt
     2461 cagttttaaa agcttccggt ttagaatcta taaattctgt agcttgagct atttcagctg
     2521 ctgttctcat ttcttcatct gttgcgtctt ttctaccata ttttaagttg gattctatag
     2581 taccagaaaa caatactccc ttttgaggta catatccaat tttttccctt aaatcatgtt
     2641 gagaaacatt ttttatatta actccaccaa gtaatatttc tccttcagta acatcaaaga
     2701 aacgaggaat taaatttata agagttgact taccactacc agtacttcct ataaaggcag
     2761 ttgtttctcc tggctttgct gtaaaagata tatttgttaa tataggttct tctgcgtctg
     2821 gatacatgaa cgaaacattt ttaaattcaa caagaccctt tttatcttcc ttaaactctt
     2881 gtgcctctga tttttctggg tcttttatgc ttatatctga cactaatact tcatctatac
     2941 gtgtagctga aacggatgct cttggaagca taatagatac cattgatatc attaagaatg
     3001 acattattat ttgcattgta tattgtataa atgccatcat atctccaact tgcattgctc
     3061 cagaatcaac actgtatgca cctctccaaa ctataagaac tgtgataagg ttcattataa
     3121 gcatcatagc aggcatcata catgtcataa ctctatttac aaaaagattt gttttagtta
     3181 aatttttatt tgctttatca aatctttctt cttcatgttt ttcagtacta aaagctctga
     3241 taactggaat accagtaatt atttcacggc ttactaagtt taacttatca acaagctttt
     3301 gaaccagttt aaacttaggc ataaccactg agaataacac tacaattaat gataagatag
     3361 caactactgc tactgctata atccaggcca ttgaagtatt tgtgtttaaa acctttatca
     3421 ctccacctat agccattata ggtgcataga atactattct aagcaacata accatcaaca
     3481 tttgtatctg ttgaatatca tttgtactac gagtaattaa tgaagctgta gaaaattcat
     3541 tcatttcagt atttgaaaat gacataactt ttctaaatac tccacttctt aaatttctac
     3601 ctacacttgc ggctactctt gctgctaaaa atcctactgt tattgtagct accatactaa
     3661 tcattgctat accaagcatt ttagcaccag taaataatat ataattattt tgagtttttt
     3721 ctgtatcaat acctattttc tcatattctg accttacata tgaaacagag ctttgtgtta
     3781 tcatactctc tggcatactt tcaaagcctt tgctcatctc tttagtcaat gtatctaact
     3841 gttcctttgg cattgctgat aacaatttaa atatatcaac atctccactt tgagtagctt
     3901 gtggtggaag tttactcatt aactgagctt tcatagcttt tacctcttta ctatcgccct
     3961 caagccctga tactattaaa atagctttac caaaaataac atttaaatca tctatagtgt
     4021 ctttgtcttt agtatttaac tcataaatag attcatcttt tagacctggg tacttatctt
     4081 tatacttaac ataatccttt ttattcagca aagtataatt atctagtact ttatccttac
     4141 tacttttatc cataaataaa gtaattttat ttaattcact ctctctaatc acagatggaa
     4201 cagagttttc tactccacct tgttgtatac ctacatttac tatattggat gtgtattctg
     4261 gtaaagaaag gtcacatacc gcttgaatta ccaatagaca gactattatt actattgata
     4321 atgccgattt ctttaaatat ttaattaatt taaacataca tttcttccct tctgtaatat
     4381 taattagtct aataacatta aattgaaaaa cattatttta aactttaaaa atatttttca
     4441 tcttcatgct tatgacatcc tgcttttaaa ttatctcgta cttgcattag aagtcttcta
     4501 agtactattt tttcttcttc actaaatcct aaaaaaaact ctttttccaa ttccttgtta
     4561 aattcttcaa ctttattaca tatttctaaa cctttgtctg tcaaaaaaat cctagatact
     4621 ctttggtcct tttcatcttg ttttctttcc acaagtccag ctttttccat cctctttatc
     4681 ataactgtag ttgtagatgc ttttactttc cttttatctg ctaattcttt ttgactttgc
     4741 ccatctttca tctttaaaga aaaaagtatt tggggttgac catgatataa gcctaattta
     4801 tcaaaataaa aatgagttct tataaaatat attcgcatta attgcaagaa aacatcattt
     4861 aatttttctt cagaattgct attcatactt gccctcctta ttttttatat tacttagtca
     4921 gctaagtatt attttactat actgcatgca ttttttcaat atatttataa aa
//
LOCUS       DAFKMX010000230         4965 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFKMX010000230 DAFKMX010000000
VERSION     DAFKMX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.234"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(529..1608)
                     /locus_tag="LZB08_003406"
     CDS             complement(529..1608)
                     /locus_tag="LZB08_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7316869.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPATVLQIMSAERVLQDVHEVTAYLKQQFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(1692..2348)
                     /locus_tag="LZB08_003407"
     CDS             complement(1692..2348)
                     /locus_tag="LZB08_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316870.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTIRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            2670..2876
                     /locus_tag="LZB08_003408"
     CDS             2670..2876
                     /locus_tag="LZB08_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316871.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(3029..3457)
                     /locus_tag="LZB08_003409"
     CDS             complement(3029..3457)
                     /locus_tag="LZB08_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBM7316872.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(3933..4871)
                     /locus_tag="LZB08_003410"
     CDS             complement(3933..4871)
                     /locus_tag="LZB08_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBM7316873.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKKLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         1510 a         1011 c          639 g         1805 t
ORIGIN      
        1 ataaaattca atagtaagtg gaataaaatt ttatatgaat aagaacatta taaaaatact
       61 ttatacaata gtaaataaaa gatattagct ccaaattatg ttaaagttgt attatttaaa
      121 gtgaaaaaac aaataaaaaa gaaataatag tataagtaga aaaatatact taaaacaagg
      181 cgaagcctct cttgtatcta aatcaagtag agaattaaat ctaaaaaaga aaataaatat
      241 aaaagatgac tcctgtacat tacaagaatc atcttatggt ttcataatca ctccatggtt
      301 tttaactgtc tgatataaag gtctcagttc actcatagca tgaccaccct ttccgattgc
      361 tgaccccgtc acttatggac aaaatgtcca taagtgatag tcctctctta aaaccctcta
      421 aatagatata tctcaagcac ctcattgaaa ccagttgctg gaaacaaact gaagcgttct
      481 ggaatagtaa aagaaattgc caccatatcc acgttactgt cctagttgtt actttgctaa
      541 cttcggagcg atttcctcta acaatacgca gtcaaattct gcctttttgc taagaaatgg
      601 cagatggtct gcatctggta taaaaaaaat ctgtttgtct ggcgccacta tttcatcaaa
      661 aaatttcttc gctggaagat agggactatt atagtcattt tctcccatga tgtagtatac
      721 agggacctga tagtcaactc cataatttct tatgtcagct tcttccacta aaaaccgcaa
      781 tacatcacct tgattgtcaa tcgcatttgg attgacaatg gccgtatttt ttaactctct
      841 caatgaataa tacggggttg ccagtatact gattatattg cccaaatcca tatttataga
      901 aagcccatac ttgctttggt atgttcttac tgtcataaga gctgccaaat agcttggacc
      961 atattttgct ggtggatatg gtgcaatagc ttccagcgcc ttgatatctt tttgcttccc
     1021 tgcagctttc accatttcaa gtgtcttttc atagcccacg cgctcattgt ccaccatatt
     1081 aacggtctgc gcaatcccaa tgtacgcgct gtaatcctcc ggataggttt gcacagccat
     1141 agttcccagc actgttcccc agctatgacc tataaggata attttctcct tgttgaactg
     1201 ttgcttcaaa tatgctgtca cctcatggac gtcttgcaga acacgctccg cgctcatgat
     1261 ctgcaaaact gttgccggat cgttcgcaaa gtatgttttg cctacgtttc gctgatccca
     1321 gttcaccaca gtaaaatcat tttcccactc atactggtac agatgtaaaa atggcatcat
     1381 tgaattacca ggacccccat gaacccaaag gatgatgggg ttgtctgcgt tttgtccccg
     1441 gatatataac gcctgctgta tgccgccgat ctccaccatt tccatgcggt cgattccagg
     1501 agcttctatt ttatggcttg tacgtataat cgtgggggct atcagacgca aaccggcaaa
     1561 cgccagaaca aaaacagcaa ctagtatgca aagtgtgatt aatatcattt taagtacctt
     1621 ctttcatgaa tttttttagc catagatatc tcctctaaca ttaaaaaaag tatgttgcta
     1681 ttttagtttt gttactctac ctgtcccatt ggtttccttt cataaaaaat attaattgat
     1741 tctataatct catcttccag gatatcaact cctatttgtc tcataagaaa ctgtaagaat
     1801 tgaaaccgtt tacgaattgt aggaaagtcc ccctgaaata catctgtatc acaccaaaag
     1861 ttaaagagca gcaaaattac ctctgcgcat tcatctggga actctgtgat gatgcttcta
     1921 tcctctatgc cttctcgtaa aacatttgct aatattggtg ctagtttttt caaattatcc
     1981 tgtgtaaatg ccagtatgat atgcggactt tctatcgcgc ttgtaatcat gttactagat
     2041 tcttttatca cctcatccgt taaatgccgc ctaactaaac ttctaagctt ctcttttgct
     2101 gtaagtccat gcatttcacc gagccattga tttactgtct ctattatctg ttcaaagcgc
     2161 ctttccatga ccacattaaa aatatcttct tttgaactga agtgatgaaa aattccgccc
     2221 tttgacattc ccgaagcatc aacaatattt tgcatacttg ttttgtcgta tcctttttca
     2281 gcaaacaact ttgcagaaat ggtaatgata ttttcaatag cttgctttgg attgttcttt
     2341 ctagtcatta tataactcct tcttattaaa aaccgactgt tggtttttat tatataagct
     2401 attcctgctt ctgtcaagca acattatgtt tatggcaaaa caataaggtt tggtggttag
     2461 acaggtatta attagactaa tgcgactttc ccctagatgg aacccttggt aaatttgcaa
     2521 tctttaccgg ctttgattat atctgtaagc aaggcaggta tggccacacc tgccctgttt
     2581 gaccctcgga tattcattcc tatccctcct ctccttagcc attacaccaa aacgccgctc
     2641 attgtctcca atggcggcgt tttaaagata tgatttctca tgctgctgaa atatgcaaaa
     2701 aatataagat gccctttatt atttttccta caatagaaaa gaaaagtatt gaagaatggg
     2761 ttaatattgg agcaaatatg ctttgttttg cacaagatac tacctttatc acttatgtta
     2821 tcaatcaatt attatctttt tataatgaga ttgtaccaat tcaaaaacaa cattaaatta
     2881 taaatctaat tttatattaa aagagactaa tattttatct atctctatta aggatatttt
     2941 cttatgtatc ataatattag tctctcttat tattatatac tgctatcatc attagttgct
     3001 atttatacta tttttataat atacaaattt attcagctaa cgttttgcct aattctatac
     3061 actcatcttt tgcttcttca tttgggtctt ccattgctgt aattccttct ccaccaataa
     3121 ttgtagcacc agcattttgc atacgttctt cccagtctct catccattca caatttcccc
     3181 atccatatga accaaataat ccaatctgtt tacctgatac catagattct aattctgaaa
     3241 caaatggctc catatacccc tcttctagtt gctctgctcc catagatggg caacctaatg
     3301 caaatttatc ttcttctttt aaatcacttg gtttcagtaa gctcacatct aaaacttctg
     3361 gtgtcttacc ttttaacttc acaccttcag ccacaaaatt tgccattttt tcagtatttc
     3421 ctgttccact ccaataaaca atatatattt tactcatttt tacctcctgt agtatttttt
     3481 aattaatcaa tagttagtta tagctaacta acatctaaaa aacaaaagat ttctttatct
     3541 gaaatctctt tatttttcta aatatagaat atcatagttg attttgaaat tcaatactta
     3601 ttgataatct ttatcattta tattttatat tcattttata atatcaaaac actgtatcgt
     3661 atttctaatc tttcatagaa aaatttctaa cttatagtac ttttgcatct cttataaatc
     3721 ttttatatgt ataagtacat agaaaaacac accttttcca gatgtgctat ctataatatc
     3781 gtatatttat ttttcaattc taactagcta atatgtttat aaattttatc acttaatacc
     3841 tttatacatt gatgtaaaat cttacaccta agtaattttt attatttgtc tcgactctat
     3901 ataatatagc tattttatca tcaaatcaaa aattatcttt tgccattatt cttgtattta
     3961 taaagattta aatataagta tccatttcct tgttccgttc taggtattcc aagcgctctc
     4021 gtacatttta ttaattgtgc atacagttca gcttcatcta aatctctttc aaactcctct
     4081 cttgcccatt ctttaattag tgctaatgag cctgatacgt atggtgcact catactggta
     4141 ccactcataa tagcaagttt attatccata taagtcgata taatatttct tcccggagct
     4201 accaaatcta ttgtagtatt tgaattacta aacttttcaa ctaaatagtt ttcatttatt
     4261 gcacctactt ctattacctc agcataactg gctggataac tatactcact tgtactagaa
     4321 tcaccatctc cattattacc tgctgcacac actacagaaa tattattttt tactgcttgc
     4381 atgacagcat tttttaactt cttatcatct ttgtttcccc caagagacat agatataata
     4441 tcaaccttgt tatttacagc aaagttaata gcattaatta tactttgata tgttccagta
     4501 ccatctttat ttaatgcttt tgctattaat aatttacaat ctggagctac tcccataact
     4561 tcattattat agttagatgc agctataata cccgccacat gagttccatg accattaaaa
     4621 tcctcatata tatttttatt tccattactg tcatcactaa aatttgcacc accaattatt
     4681 tttcctttta aaagaggatg agatatatca caacctgtat ctattatacc aactacaata
     4741 tttttaccag tataaccttc atcccacatt cccctagcat tcatttgttt tattccatat
     4801 gggaatttat tttttgcacc tcttagattt tcatttattt catacggaat tagtttcatt
     4861 cttttattca tcatatatcc tcctaaaaat tttattgctt ataaaaatca tgaattttat
     4921 tttataatag gtttataaat caaattaaaa cctatatcta cttat
//
LOCUS       DAFKMX010000231         4895 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFKMX010000231 DAFKMX010000000
VERSION     DAFKMX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.236"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            513..1373
                     /locus_tag="LZB08_003411"
     CDS             513..1373
                     /locus_tag="LZB08_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7316874.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1363..3234
                     /locus_tag="LZB08_003412"
     CDS             1363..3234
                     /locus_tag="LZB08_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBM7316875.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3254..4708
                     /locus_tag="LZB08_003413"
     CDS             3254..4708
                     /locus_tag="LZB08_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBM7316876.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1884 a          511 c          894 g         1606 t
ORIGIN      
        1 ttatatatta gtatatctta ctagcattta aatgaaacta aatttgaagc tttacctatt
       61 aatatatctt aatagtgatt aaataaaact aaacttaaga ctttatatgt taatatatct
      121 taagaattta tttgttaaca tacagttcat tatatttaaa agcattacta tgataattca
      181 taatagtaaa atagataatg gttcagacaa tattataaat attagtgacg gtaaaatagt
      241 atcaatttag tatataaacc ttgtcagata atcgtataac agagcttgta gttatagaat
      301 aaaaaaatat ctaggaaacc gttgacagat ttatatggat agtataacat ataaaatatg
      361 aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc ataattttga gtatgttact
      421 gattagcagg cagaactcaa gtatatatgt gaaaaataga gggatactct atacatatat
      481 gcttgagttc tttttattga aaggaggaat atatgaaaat aattcagatt ttaaataata
      541 atgtagcctt agtaaaaaga ggtaaaaatg aaattattgt atttgttaaa ggaattggct
      601 ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat agagagaaca tatatactag
      661 attcatatga tatgctagaa cattttagtt atctactaca aaactcagac tccaataata
      721 tagttcttat taatgaaata attgaatatg gtgagaaagc tttaaatata aagtcaagtg
      781 attatctaag cttaacctta ctagaccatt tagagttttt acttaaaaga tgtgaaaaga
      841 atcaatttat aaaaagtcct ttggtatgga atgttaaaag attttatcca aagcattttg
      901 aaattggaat gcatgcacta aaattaattg gtgatagtaa gggaattaat cttcctgatg
      961 atgaggcagt atcaatagca cttcattttg taaatatgga ggtaaataaa gagtctcatg
     1021 atagtacaat tgtagagttg agaactttag cagatatagt ttctataata aaatatcatt
     1081 ttaatgtaga gctagatgaa acttcaacaa attatatgag atttactact catctacagt
     1141 actttatcca aagaattatg tcagatgaag cttcaataga tgaagataat tctatagatt
     1201 tatataaaca agtaagtcaa ctttatccaa agtcatttgg aagtgttcaa aagataagaa
     1261 aatatataaa atcacaattt aatgtcgata tatctataag tgaagaaact tatttaatgt
     1321 tgcatataaa tagggtgact caaagactgg aggcaaaatc agatgaaata taaagattta
     1381 aatgaaaata taataaaact tataggggga aaagaaaata taaagtcggt agcgcattgt
     1441 gttactcgtt taagatttac tttaaatgat aggagtatcg ctcaaacaga agaaattaaa
     1501 aaattaaaag gagtaattga tgtagtttct aatgatgttg cttatcaaat tataataggt
     1561 actcatgttg tagatgtata taacgagttt atgtctatga taggtttaag ttcaagtgaa
     1621 tcaagtataa aaagtgaaag aaaaaaggga attagaggta tcaaaggtat atttaattcc
     1681 atctttgtgg tagtctcaga gactatgact tcagtaattg aagtactact tgcagctggt
     1741 atacttgcaa gtatattagc acttttaaat atgtctggca tacttcagag tgacagtcct
     1801 acatatcaaa tattagatac attaagaagt gctacatttc acttcttacc agtattaatt
     1861 gcaatagcat cagcaaaaag acttaatgta aatccctata tagcagtagt gttagcagtt
     1921 acgttattat cttcatcaat agatggtgta gaaggattaa gtttatttgg tataaattta
     1981 caggctacaa cttatgcaaa cagttttatt ccaatacttt taggagtatg ggcaatggga
     2041 attataattg aaggtttgaa aaaaatatta cctaaagcac tacactattt tttagttcca
     2101 gtattatctt taataataac tctatcagtt acattaactg tatttggacc tattggtatg
     2161 tggattggtg atggattaaa ctatgcatgt atgtttttaa ttgatacatt gggtaattgg
     2221 agtgttgttg cactgtatgc agctttacaa ccactcttaa tagtttttgg tgcagcaaac
     2281 tttactatgc ctatatcttt aaattctgtg acaacattgg ggtatgaccc aatttttatg
     2341 ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa tgtttggata tttcttaaaa
     2401 actaggaata aagaacaaaa acaactattt ggtacagtaa gttttagtgc acttatggga
     2461 gttacagaac cttcagtgtt tggtgtgttt atgaaatata gaagaccatt tgtagcagta
     2521 atgataggtg gaggtatagg tggtttaata gcaggtttag ctcaagttaa aacatatgga
     2581 atggtttggg gattagctgc attaccaaca tatcttgttg gaggagaagt atctaacttt
     2641 acatttatga taataagtgt agtagtatca tttgtagcag caacagcagc ttcctattta
     2701 ctaggaatac cttcagatga aaaagaagaa gttaagggtg ataatgaaaa agaattagaa
     2761 ctaataatca acgagaacaa ttcaagaact attagtattg gaaatgttgc aaaaggtcag
     2821 gtaattgcac ttggggaagt taatgacaaa gcattttcat ctggagcatt aggtaaaggg
     2881 gttggagtta tacctagtga atcaaaatca actgttatat ctcctgtaga tggaactata
     2941 actatagtat ttccaactaa acatgcatat ggtatagtga ctgatgaagg attagaaata
     3001 ttaattcata ttgggataga tactgtaaac ttagatggaa aattctttga aagtcttgta
     3061 acacaaaatc aaagggttaa aaaaggagat gctttagcag tatttgaatc tgataaaatt
     3121 atagaggaag gctttgattc tactataata actgtagtta caaatactag cgattattta
     3181 gatgtaatct catcaaatga tgaatcagaa gaattattaa ctgtaatagt ataaatttta
     3241 ggagggaaaa ttcatgaata ttaaagatac atttttctgg ggaggaagta ttgcagccca
     3301 tcaatgtgaa ggttcatggg atagtgataa caaaggacct gcaattatgg attttgtaac
     3361 taagggctct tatgaaacgc caagagtaat tactgataaa atagaagaaa aattggatta
     3421 tccatctcat aatggaatag atttttataa tagatataaa gaagatatag ctttatttaa
     3481 agaaatggga tttagtgcat taagaatatc tatagattgg tcaagaattt ttccaaatgg
     3541 agatgatgaa aatcctaatg aattagggat taaatactat gaaggtctga tagatacatt
     3601 aatagaaaat aatatagaac ctattgtaac actatatcat tttgaattac ctatgaattt
     3661 agtacataaa tatggttcat ggaataacag aaaacttata gatttgtatt taaagtacag
     3721 tgagactgta ataagaagat ttgatgataa ggtaaagtac tgggttacat ttaatgagat
     3781 gaaccatatt gaccctcaaa cagagcactc ggatattttt acttatatga tagcaggtat
     3841 taagtatagt gaaatagaaa ataaggttca aacattggca aatgtagggt ataatatgac
     3901 tttggcgggt gtaaaggttg caaagttggc tagagaaatc aatcccaaca ataaagttgg
     3961 atgtgtattt ggactaaatc caatttatcc atacaattgt aatcctagta atgtgttaaa
     4021 tgcattttta gataatgacc gtgattatta tcaaattgat gcaatgtgca atgggaagtt
     4081 tccacaatat aaattaaaag aatatgaaaa aaataatata actattgaag tgactgatga
     4141 agacaaaaaa gcatttaaag aaggtaagtt agattttata ggacttaatt attatttttc
     4201 aagtgtatct actccaaaag aaaatttatc aggtgataaa tctttatttg gtgggattca
     4261 aaatccatat ttggaacaaa gtaagtgggg gtgggcaata gaccctatag gtttaagatt
     4321 taccttgaac tatatttaca gaaagtatca attacctatc ttaattacag aaaatggttt
     4381 aggggcatat gataaaatcg aagaagatgg aactataaat gatgattata gaattgaata
     4441 tttaagtaag catctatctc aaatgaagtc tgcaattgaa gaggataatg ttgaatgttt
     4501 tggatatttg atgtggggac caattgattt agtcagtgca actacaggtc agatgagtaa
     4561 gagatatgga tttatctatg ttgacttaga tgatgaaggc aaaggaagtt ttaagagata
     4621 taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa tctaatggag aagttttaga
     4681 tttttctacg gatatagtag tagggtagga ataagataaa tagaagttag gaactaatgt
     4741 taattagtgt ctagtaaagc atttaaaata gagtataggg tctttaaagt aaagtgtgta
     4801 ttcttattaa aagtaatggc aactaaaatt actataaaaa ggagaataca cattttttat
     4861 gtttatactt aagtaaatat ttagacatat tcata
//
LOCUS       DAFKMX010000232         4861 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFKMX010000232 DAFKMX010000000
VERSION     DAFKMX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.237"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            89..865
                     /locus_tag="LZB08_003414"
     CDS             89..865
                     /locus_tag="LZB08_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7316877.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            881..1246
                     /locus_tag="LZB08_003415"
     CDS             881..1246
                     /locus_tag="LZB08_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7316878.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(1412..2161)
                     /locus_tag="LZB08_003416"
     CDS             complement(1412..2161)
                     /locus_tag="LZB08_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBM7316879.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            2639..3064
                     /locus_tag="LZB08_003417"
     CDS             2639..3064
                     /locus_tag="LZB08_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316880.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLVKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            3096..4283
                     /locus_tag="LZB08_003418"
     CDS             3096..4283
                     /locus_tag="LZB08_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBM7316881.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            4475..>4861
                     /locus_tag="LZB08_003419"
     CDS             4475..>4861
                     /locus_tag="LZB08_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBM7316882.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLK"
BASE COUNT         1879 a          546 c          782 g         1654 t
ORIGIN      
        1 tggtaatcaa attctttacc aatgaatata aaaaatgtct acgtgttata atagtttaaa
       61 ctaataaacg aaaggtagat gaatgaatat gaaataccaa catataatag aagttgacaa
      121 agaactttgc attggatgtg ggctttgtaa aaatgattgt ccagtaaaca atataattat
      181 agaaaataaa aagtcagtaa ttaaaaaaca agattgtcta atgtgtggac attgtgcagc
      241 aatatgtccc acaaaagcta ttaccttgac agggtttgat gaacctccta tagaacttac
      301 aaataagcca aagctagatt cagatgaatt actaatggca attaaatcta gaagaagtat
      361 acgtaaattt aaagacaaag aagtttcttc agaaattata aagcaaatta ttgaggcagg
      421 taggtatacc cctagtgcta aaaattcaca agatgtatca tatattgttt tagacaataa
      481 aaagtcaatt tatgaaaacg aagcagtgaa attttttaga aaaataaaac ctattgcaaa
      541 tatagctatt aaatattcaa aagaagttga gattgacgat aactttttct ttaagcatgc
      601 tcctattgca ataatgataa ttacaaaaga taagattagt ggttcacttg cagcatcaaa
      661 tatggcatta atggcagagt cttatggcct aggtgtttta tttagtggat atttttcaga
      721 tgtagcaaat aattcgccaa aattaaaaaa gcttttaggt ctaaaacgca gtaatcatgt
      781 tcttacaaca ttagtaattg gatacccaga tgttaaatat agacgtactg cacagaaaga
      841 agtagcaact gttagatact tataagagag ggtggatttt atggaaaaag aatgtcaaca
      901 aagaatgcaa gaaataacgt ctaaatttaa agaatgtcgc aaagctatct ctgctatggg
      961 tgatgagact agacaattga ttttaattgc tctcttagag agtgatttta atggtatacg
     1021 agtgggagaa atcacaaaaa aaacacatct ttctcgtcct gctgtatctc atcatcttaa
     1081 gatattaaaa gaagcagaga ttgttaattt acgtagagaa ggaacgaaaa attattatta
     1141 tcttgattca aaaaaatcac aatggaagtc actaacagag ctaataaatc ttatatttgt
     1201 tggaatacaa catattagtg aaaatgatat aagaaaaggt gattaaatta tacttgattt
     1261 aatacaaaaa gattgattta ggaacttaaa agttcacttt gagacactat cttttgaact
     1321 atacccatct taggagacag ttaaaatatg taaaactgta taaaacaatg ggtatagttc
     1381 attttataat ggtttaaata ttaagaatat attattctaa atagtatctt tctctgtttt
     1441 gagttttatt tttatactca atagcttcag tggggcaacc acaaatacat gccatacaat
     1501 gagtacagtt atttttccaa actggttttt tgttttttaa attaatatta tttaaaggac
     1561 atagctctac acattttcca cagccaatgc atttatcagt ataatgaaat cctttggcac
     1621 taataattgt cttataaaaa actgtattta ttataccact tttaattgta cctcctaagc
     1681 cactaggagt aatagtttca aattcgtttt cattgcgtat atcagaaata atcctatata
     1741 tttgtttatc tgcttcttta atcatttgtt ttgatgtttc tttgtctgtt gtagaaaata
     1801 aagctatata attctctggc attttaattt ctgccatacc ttttaattgc cattccttat
     1861 atttacatag tttttgaata tagtttatgg attttgctgt atctccacca caagtcatta
     1921 caaagtaaac tctgttgctt cctagaaatt ttgtttcttt tataaaatcc gtaaccacta
     1981 ttggcaatct ccatgcatag gtaggacata caaatatgaa tggtttatct gctgatatta
     2041 attcatctgt tttttcctct tttataagtt gatttagtga tatcagttca tcatttaatt
     2101 cttgtgcaat tttacgagcc acatatcggc tattaccagt tccgctaaaa tataaaatca
     2161 taataatatc tccctaacta ttttttattt taactcttaa ttttttattt attatagtaa
     2221 tttattgata tattctattc aaatgtatgt aaaatttagt agttaagaaa attataacac
     2281 attagtaatt tttatactta ttggtaaaga atttgattac cattaaattt agcaacaaga
     2341 catcaactgg acttgtatat gagaagggat aataatttgc ttatgaaaga ttataaatat
     2401 aaaggttatt ttgaaagtgt agaaatattt gataatgtaa atgaccttaa cttaaatact
     2461 ttaacaacag ttagtagatt tgtgcttgtt tttttgttta cgataactta aggaaatatg
     2521 ttacctgaat aataaattta tataataaaa gtatataaat atatatatga atgttaagat
     2581 tagttgatag aatttaaata ataaatccaa tattataaac ataggaggta tgaaaaatat
     2641 gattaatgag aatttaaaaa gtctaagaaa aatcaaccag tatacacaag aagaattagc
     2701 tgaaaaactt aatgtttcta gacaatcaat agcaaaatgg gaaagtggag aatctattcc
     2761 tgatatatgt agttgcatta aacttgtaaa attgtacaat gtcaaactag atgacttagt
     2821 taatcattca gaagaaaaaa caggtattat agtacctcct aaaggaaagt ttttctttgg
     2881 agcagttgta gttggtgaac gtggtcagat tgtcattcca aaagaagcaa gagaagtatt
     2941 taatataaat gcaggagata aattattggt acttggagat gaggaaagag gattaggaat
     3001 tgttcatcaa cgtgatttaa taaattttat aggtcatgtg ggagtagtac gtaaagaaga
     3061 atgattttaa aaaattataa aaggaggtat gaaatatgag taaaatagta ttttttagca
     3121 ttcctgcata tggtcatact aatcctacta ttgaagtagt tcgtgaatta gtggatagag
     3181 gaaatgaggt attgtattat tcttttaatg aatttaaaga taaaattgaa ggtgctggag
     3241 caaaatttat ttgttgtgat aaataccttc cagaattact ccctggagat gagaaaaaaa
     3301 ttggtaagga ttttcctgga ttaattgaga tgatagtaga tactactatt tctttagatg
     3361 aaaaagtttg tagggaactt aaagatttta atccagattg cattgtatca gattcattaa
     3421 gtttttgggg aaagttattt gcacaaaaac ttaatattcc atatgtatgc tctacaacaa
     3481 cttttgcatt taataaacat acagcaaagt tgatgaaaca aaactttata gaaattatac
     3541 gtatgatttt tggtgtaaga cgcattaata aaaaaataaa gcttttacaa agaaaagggt
     3601 atgaagtaaa aaattttatt tctatagttt ctaatgataa tgatacggat accattgtat
     3661 atacatcaaa agaatttcaa ccaatggtag aaaccttctc tagtaagtat tcatttgtag
     3721 gtccatcagt atcaaagttg attattgaac ctaaagagag aaaaagaaag ctaatatata
     3781 tttcgcttgg tacaattaat aataaaaatg ttagctttta taaaaactgt ataaatgcat
     3841 ttaaagattc tgatgttgat gtgattatgt ctgttggtag aagtactgat attaaaagtt
     3901 taggaaatat accaaataat tttgaggtta aaaatagtgt tgaacagatt gctatacttc
     3961 aaaaaactga tgtgtttgtt actcattctg ggatgaatag tgttaatgaa agtttatatt
     4021 atggagtacc tatggtatta tttcctcaac attcggagca gagaatggta gctaagagag
     4081 ttgttcattt gggagcagga attatgttaa aagaggataa atcagaaagt attaaaaaag
     4141 cagtatttca agttataaat gacaatgaat ataaagaaaa tgcaactaaa ctatcaaaaa
     4201 gcttttataa tgcaggaggc tcaaaaaaag cagcagatgt aatattacag attatatgta
     4261 atagtagaca agttgacata taatagattt gttaaaaata tatatacata catgtaattt
     4321 aatcaaagta ctgacagtgg ggtcttttaa cctaccagtt ggggcatata tattgatgct
     4381 aaatatgagg gatgttatat tttaataaaa aagataacat ccttttttta gtgcattata
     4441 tcgagtttta aataataaat gaaggaggat taatatgaaa aaatataaag ttatttttat
     4501 agtattaatt ataggtatta taagtgtaac agggtttgtt gtatataaaa actgcattaa
     4561 accatggaaa gatgctgaaa catatattga taagtatatg gtaaaacaag atatttcaaa
     4621 agataatata aaaagtatta caaaagaaaa agccaagaag gcaagttacg aaggaatact
     4681 atacaaagtg tattacaaag atgaccctcg atatgagtat caatattttt atagtgataa
     4741 ttattatgct ctatatgtaa ataaagtaat gttacaaatt tatgatatgg aaaataataa
     4801 taaactttta agaagtaatg aggaattaaa aagtgtaaag tatccaccaa tatatcttaa
     4861 a
//
LOCUS       DAFKMX010000233         4826 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFKMX010000233 DAFKMX010000000
VERSION     DAFKMX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.238"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            205..711
                     /locus_tag="LZB08_003420"
     CDS             205..711
                     /locus_tag="LZB08_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBM7316883.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1124..1792
                     /locus_tag="LZB08_003421"
     CDS             1124..1792
                     /locus_tag="LZB08_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316884.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1951..2457
                     /locus_tag="LZB08_003422"
     CDS             1951..2457
                     /locus_tag="LZB08_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBM7316885.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGNRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2585..3601
                     /locus_tag="LZB08_003423"
     CDS             2585..3601
                     /locus_tag="LZB08_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBM7316886.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3955..4659
                     /locus_tag="LZB08_003424"
     CDS             3955..4659
                     /locus_tag="LZB08_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBM7316887.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         2048 a          417 c          809 g         1552 t
ORIGIN      
        1 tatatacttt ataatcaata tattttatct atattttaca cttataaaat atgaaaacta
       61 cttattgcga aagtaattga tttgttgtat tataatttac atgaattatg taggacaatc
      121 aaaagtcagt attataagag tacatatatt caaattttta acaattaatt tatataatta
      181 aaaaatgaag ggtaggatga agtcatgggt tttagagaag taaaaattga ggaattacag
      241 tttaatccat ttactaagat tggcaaagaa tggttactta taacagctgg tgattcagaa
      301 aagtttaaca caatgactgc tagctggggt ggtgttggag tctattgggg taaaaatgtt
      361 gttacaactt acataagacc acagagatat acaaaagaat ttgtagatag taatgataca
      421 tttacagttg ccttctttga tgaaacgtat agagaggctt taaatatttg tggtactatt
      481 tctggtagag atataaataa aatagaaaaa gcaggactta caccatattt tgtggatgat
      541 acagtagctt ttgaagaagc taatatgatt atagtatgta aaaagctata tcatggtaat
      601 atgccaccag aaaactttga tgctaaagaa aatgataaaa aatggtatcc agagaaagac
      661 taccatacta tgtacatttc agaaattata aaagttcttg taaaagaata attataataa
      721 aaaatatatg aaaagaactt attataagta aatgactgtg ctttcttatc tgatatctaa
      781 gtagatgcta gtaactaaat aaatattaat tttaaataaa tactattata aaaaagatga
      841 actatattta ctatagttca tctttttatt tatataagca cttatataag tagagattta
      901 tttgaataaa agtatttatt tgaaatattt gagtatactt tataagtatt tttatgctaa
      961 ctagaaacac ttaatttatg gcatttgtac ttgatttatt ttaatgtaaa taatttcaaa
     1021 gttaatttta ttcaatatag tactataaaa gtataaataa catttaataa aaatatatat
     1081 tatattaggt attttaaata aattttttaa agggtgattt tttatggaaa atataactag
     1141 ttattttaca acaatactat gtgtatttat atgcttatca tctgtattta tatttactca
     1201 actagctaga gtatttatta ataagaagaa gattaatcaa aaaattaaat caagaaacgg
     1261 atttaggtat gatagagatt ttattgaagc cagaagagaa gaaatacata taaaggataa
     1321 caataataat aaaaataaga gtaacaataa gaaattaaag gaagaaaaag tatttaaata
     1381 cgataatggt gatttatata aaggtgagtt tgttgatgga aagaaaaatg gatttggaat
     1441 atatattttt tcaagtaagg aaaagtatga aggattatgg aaagatgaca agatgcatgg
     1501 aataggaaaa tatacttata gagatggttc aatatataca ggtgaattta aatacggatt
     1561 aaaaaatggt ttgggaaaat taacatatcc caataatgac atttataagg ggtatttttt
     1621 agataataag aaaaatggta agggtgtatt atataaaaat gatggaaata aacaagcagg
     1681 gatatgggaa gatgatgaac aatgtaaaag tttagatttt aaagatttaa ataataataa
     1741 aataaataat gtttatcaaa atagattaaa taacaataaa ttaaaagttt aattattcta
     1801 ataaaaaagt ctttatttat gatggaatat aggatatatc tatgttgtaa tttgtaaaat
     1861 cttcctatat catataaaat taagtattga ttagtctatt acataaatat agaatataaa
     1921 tatatccatt taataataag gagaagagat atgaaagagt atgtagttgt ggatttagaa
     1981 actacaggat tagatccata taaaggttgt gaaataatag aaataggaat aacagaaata
     2041 aaaaatgaac aaattgtaaa aaactattca aggttgataa aacctaaggg gataatttct
     2101 tcatttataa cagaattgac acatataagt aatgaaatgg tagaaaatga agaaccacta
     2161 gaattagttt tacctagatt tagaaaatac ataggtaata gaactataat tgcacacaat
     2221 gcgaaatttg atttaaaatt tttaaattac tatcttagaa tgttagattt agaaccaata
     2281 aacaattata tatgtacagt agagttatta aaaaaatgta aaagttataa aggaaaaaat
     2341 aagaaacttg aaacagcttg tgcttattac aacatagaga atataaatgc tcatagagct
     2401 gacagtgata cattagcaac tgcaaaacta tttttgaaaa taaaagatga atattaatta
     2461 taaatataga gaaaattagt gaaaatatcc tttataaaat tgacccttaa acttacaatt
     2521 ttgttctttg atttattttg tagaacaatg tataatgtac cttatatgaa gaagggagac
     2581 taaaatgggt tattacaatg ataatgataa taataaaata agacgtaaaa aaattagtaa
     2641 caacaaagat agtaataaaa aatctaaaca ttcttatact tcggcaaata aaaaaaataa
     2701 aagtcctaat acaagttcca gtaaaagaat gagtgaaaaa gtagattcac agaatcttat
     2761 aaaaagagat agagatagtt ttacaagaga taatgtaaga cctttaaata cagattacaa
     2821 tcaaagagaa agaagaagag tcgaatataa acaaagagag aaaaaattaa aaagtaaaaa
     2881 aagaaaaaaa caaaaaagga ttagaatagt tagggcatta ttatctatta tgttagttgc
     2941 actaattata ttttcagtgg caggtgcaat atttacagtt tcagctataa aagggtcacc
     3001 agaagttact aaaaagttaa ttaggtctaa atatataagt agtgaggttg taagtataaa
     3061 acaaatgcca aatgatttaa aacatgcaat agtttccata gaagatgaga gattttataa
     3121 gcataaagga gtagatgtta tttctctagt taggtcagta ttaaacaatg tgtttacaga
     3181 tacaacacaa ggtgcaagta ctatagatat gcaagtttca aaaaacctgc ttactagtaa
     3241 tgataaaact atgaagcgga aaataaggga tatgtataat gctaagcaga tgaataagat
     3301 tatgactaag gatgaaattc ttgaagcata tttaaataat atgtatctag gtaaaaatgc
     3361 atatggagct gcaaagggag ctgaagtcta cttcggtaaa aaagttagtg aattaaattt
     3421 agctgagtgt gctatgttag ctggtatgac aaataatcct gcaaggtata ttgaccatgg
     3481 agaggctaag aagagacaag tgattgtact gtataagatg catcagcttg gttatataac
     3541 tgataaggag tatagaatag caaaagctga tgacactcca tttaaatcag aaattgatta
     3601 aaattgtaag aatatagtat tttcaatagt ttaaacaaac aaaaatgtaa taaaaaaggt
     3661 gatttgccac ccatttgcca ccataattat aatctaatgg caaattgttt taatgtacaa
     3721 actagataat agaatattgt cttaaatgct atattatgct aaaatttaat tgtattttgt
     3781 aaattaaaaa acttataatt tagttttcta gggttccgca ttttataatg gtcaggtcca
     3841 agagaaaacg cacaattgtt gtgtacacgg aaggataaaa gcctgggaga tatttaataa
     3901 tatttctata ggctttttta tttttacata ttttaaattt gaggaggttt tctaatgaaa
     3961 aaatttgtta ttgaggatgc attttggaat ctatttccta gtgcaaaaat tggagttgtt
     4021 atttgtaatg atatagataa ctctataaaa gatgaagact ataagaaaat aattttgaag
     4081 ggagaagaag aagcattaaa atacttggag gactcagaat ttagcaacaa ccaagtgatt
     4141 aaagtatgga gagaagcatt taaaaaattt aaaacgaaaa aaggagcaag gtcttctatt
     4201 gaagcattat taaaaagagt tcataatgga aataatatag gaactattaa ccctttagtg
     4261 gatatctaca attttatctc attaaaatat gcattacctt gtggagggga ggatatagat
     4321 aaatttatag gagatattag gctaactaga gcaattggta atgaggagtt tatcccacta
     4381 gggacagatg aaaattcatt tccatatgaa ggagaaataa tttataaaga tgatggtgga
     4441 gcaatttgta gatgttggaa ttggcgtgaa gcagtgagaa caatgcttac tgaagatact
     4501 aacaatgcct ttttatgcat tgaattagta gatgaaagta gatttaaaga atttgaaaat
     4561 gctttaaatg aattagctaa aatagttcaa gaaaaattgg gtgggaattg taaaatttca
     4621 attttagata ttgataataa agaggtatca gtggattaat atggaggtgc tgttataatg
     4681 gactattcta gttataaaga tacttatatt tagggtaaga aggtctattt tagatataac
     4741 gattatcaaa ggatataata tagaaaataa aaatgtagaa tataaaaagc ttttaaaata
     4801 agaaaaaaat gtattttaaa ggcttt
//
LOCUS       DAFKMX010000234         4807 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFKMX010000234 DAFKMX010000000
VERSION     DAFKMX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.239"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            236..1387
                     /locus_tag="LZB08_003425"
     CDS             236..1387
                     /locus_tag="LZB08_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBM7316888.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1433..2608
                     /gene="thiI"
                     /locus_tag="LZB08_003426"
     CDS             1433..2608
                     /gene="thiI"
                     /locus_tag="LZB08_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBM7316889.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2764..3567
                     /locus_tag="LZB08_003427"
     CDS             2764..3567
                     /locus_tag="LZB08_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBM7316890.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3810..4451
                     /locus_tag="LZB08_003428"
     CDS             3810..4451
                     /locus_tag="LZB08_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBM7316891.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1924 a          453 c          926 g         1504 t
ORIGIN      
        1 ataatatatt attttatcat atattgtatt ttataatata tttttgtagg gtatagtata
       61 agtatattat attttactat aaaaatttgt aataaagtga aaaaactagc ttttcatgaa
      121 ataatatttt ataatttaag attgtctttt taataaagat taacaaaaaa aatactataa
      181 ttagtggtat aatagttaga gttgaagatt ttaaataaag gtaggtgtaa ttaagttgga
      241 aatatactta gacaatagcg caactacaaa accttatcaa gaagttatag ataaaatggt
      301 ttatgcactt aatacggaat atggaaatcc ttcttctgtc catagaaaag gtgtagaagt
      361 tgaaaaagct ataaaagaag taagacaaga tatagctaaa tctctaggag cgaaagaaaa
      421 ggagatttat tttacatcag gtggtacaga gtgtaataat actataataa gaggtattac
      481 tagtttaaat aaaaaaagaa aaaatcacat aatatctact aacatagaac atccttctgt
      541 tttaaataca ttaaaagatt tagaagaaga tggttttgaa gtaacttatt tagaggtcgg
      601 aaaagatggg aaaataaata tagaggattt aaaaaatgcg ataaagtcaa ctacatgctt
      661 agtaagcatg atgcatgtta acaatgaaat aggaactata cagccaatag gtgaagttgg
      721 aaagtatttg aagggtttaa aagaaaaaat ttatttccat gttgatgcta ttcagtcata
      781 tggaaaaatt aactttagac catctaaata caatatagat tttatgagtg taagtgcaca
      841 taaatttcat ggtccaaagg gaattggatt tatgtatata aaagaaaata atcgccttaa
      901 gcctatgtta acaggtggtg gtcaggaaat tggaattagg tcgggaacag aaaatgtacc
      961 tggaatatat ggtcttggag aagctgttag aatactaaac aaagatttag atgctgttat
     1021 ttcgaaagta gataacttaa aaaatatatt aaaaaatgaa ataattgata atatagaaga
     1081 tataaaaatc aattctccag aagatggagt atgtcatata ttaaatgtat catttagagg
     1141 gacaaaagga gaagttttac ttcattactt agagcaaaaa ggtatttatg tatcaactgg
     1201 ctctgcatgt tcttctaaaa agaaaggtag ttacgtatta aatgctatag gtcttacaaa
     1261 tgaagaaata aatggaacaa taagatttag tttatctgat atgaatacag aagaagaaat
     1321 gttagaagct gtgaaagttt tgaaagaatc aatttgtgac ttaaggtcaa taatgaaaag
     1381 aaaataaaga atactgtata aatagtgata ataaattagt ttaagaggtg aaatggtgta
     1441 taatatatta atagtaaagt atggagaaat tggagtaaaa ggaaagaata gatatatatt
     1501 tgagaatagg cttataagaa atataagaaa tatgttaaaa ccaattggaa gatttaatgt
     1561 atataaagag tatggaagaa tatatgttga tttagaagac tatgattatg aagaagtaat
     1621 tgaagaagtt agaaaagtat ttgggatagt gggtgtgtgc cctggagtaa gagctaaaaa
     1681 agattatgat acattaaaag aaatagcttt aaaaatgtta gaagaaaaaa tagaggctgg
     1741 atataaaact tttaaggtag agtcaagaag aggagataag tctttcgggt tgacttctca
     1801 agaaatgagt atggatatag gtggatactt attatcaaag gtaggagata ggattaatgt
     1861 tgatgtaaga aacccagaag ttaaaataaa gtgcgaatac agagaatttc atactatggt
     1921 ctatagtgat actatacctg gatatggtgg attgccactt ggaacgaatg gtagagctat
     1981 gtctctttta tcaggaggta tagatagccc agtagctaca tggatggttg caaaaagagg
     2041 tatggaagta gaggcagttc actttcatag ttatccattt acaagtgaaa ggtcacagga
     2101 aaaagttaag gatttagcta aaatactggc aaaatactgt ggtagagtaa ggcttcataa
     2161 agttaatatc ttagaaatac aaaaagcaat tggcgagaat tgtaatgaag aagaatctac
     2221 tattctttca agaagattta tgatgaggat agcgcagaga ctttctgaaa aaagacattg
     2281 tgatgcttta attacaggtg aaagtattgg gcaggttgca tctcaaacta tacaaggact
     2341 tacttgtact aatgccgttg tagacttacc agtgtttaga ccacttatag caatggataa
     2401 atcagatata gttgatatag ccaaaaaaat agggactttt gaaacatcta tagtgcctga
     2461 agaagattgt tgtagtgtat tttctcctag aaagcccgtc actaaaccta gattagagaa
     2521 aatagaaaaa tctgaaacag cacttgacat tgaaaaatta gtacaggatg ctattgacaa
     2581 aatagaggta gaggatattg agttttagca tacaagttat ttaattacta aattgaatat
     2641 tgagtatatt agtagttctg taagtatatt agtagttctg ttgaaaggat ttatgtagac
     2701 aatttattaa tatgtagtat ttaaaaataa gtactataat tttatttaat gggaatgatt
     2761 attatgaata aaaagggttt tacattaatt gaattgttgg tagttatatc tataataggc
     2821 attttagtta tagtagctgt tccagcgtta tttagaaata tagaaaaaag taaagcagtt
     2881 acatgtcttt ctaatagaga aaatataaag actcaaattg ttattgcaat ggctgaggaa
     2941 tcaagtaaag acaagaatga agtcataaaa gaggtattag aaaacaaaga tggtaagtac
     3001 tttgaaacag aaccaaagtg taagtcaggt ggaatatatt cagcaacgtt tgatgatggt
     3061 tatgatggaa taactggaat agaaagcatt gcaaaagtgt atgttacttg tacaaaacat
     3121 ccagacggtg ttgaaatggc tagggatgtg catcaaagta tgaaagattt gattgcatca
     3181 tttgcacaag acccttctat aataccagga gcttcaaagg gcaatgatga ttttagaaaa
     3241 tatttattag acaataaata taaaaatggg tggcctacaa ttccagatga atttaaggca
     3301 aaatatggat taagtaagga tacactatat atacaaccat atgcatataa tcctactaaa
     3361 tctgatgcta ctgtagttgt atttgcaaat aataagactg ggggtaattg gtatacttcc
     3421 ctagtttacg attatgatga aggtagatgg tataaaggta aaaatggtat ttctgttgca
     3481 ggtaggtcat gggatgttga cacagatagt gttaagtctg taaaaacaga gattcattct
     3541 aaagagggat ggggtccttt aaattaatat atcatgtttt attaatctgg tattgattaa
     3601 actatagaaa agagcagttt ctatagatga cgaataccag attttcattt atatatataa
     3661 ttgagatata ttaaaagtgt ttatattttt aagtcttcac atacaagcta aaaataatta
     3721 taataatatt gtaaagaaat ttaattataa gattaatttt ctgaagattt agtatttgta
     3781 ctaagaaata cataaaaagg ggaatagata tgaataataa ttttcaagac aaccaaacaa
     3841 taaatttaat acaaagtcgt agaagtatac gaaaatttac gacagaacaa attagtgatg
     3901 agcaagtaaa cacattatta cactgtgcat ttgcagcacc ttcaggatgt aataaacagc
     3961 cttggcacat aactgttgtg caagaccaga aacttttaaa agagattagt gatgatactt
     4021 tatctaggat tcatgaggtg agtaatgttg agataaataa aaactttaag ttattttatg
     4081 gtgctccaac cgttttattt atttcttatg atgaaagtag ttcatgggct ccatatgata
     4141 taggaatact tacagggaat ataacaacag ctgcacaagc actagggctt ggaagttgta
     4201 taataggaat ggttagaggt ttatttacac ctgttgaaca aggggatatt gaaggtcttg
     4261 tgagtgtatt ggataaagaa gatgtaaaag aatcagagtc aataaagatg aagtttgata
     4321 caaataaaaa atacagagaa ctacttgata taccagaagg gtattctgta ccatttggta
     4381 ttgctgtagg tattccagat ggaaatttac caaatgctag agaggtagtt tataaagttt
     4441 ccagagttta ggataaagta ttgcaagaac caatcagtta cttgaagaat taaatcagta
     4501 atatacaaat aaattgatgg aagaggaagc tatttaaaaa gtagtttaga ttttttgttg
     4561 taaaataatt aaataaagaa taaggtgata agtattttat ttattatctt attctttttt
     4621 atttttgttt tgtagaatga taaaaataag gattaagtgt aataaaatag aaaacaagta
     4681 tgttaatttt aagtatatgc tttaaaattt atccaagtta aaaatttaag atatcttttc
     4741 agtataatgg aaaaaatgtc aaaatattga aatagggaaa aaatagtgat ataataagaa
     4801 aaaatag
//
LOCUS       DAFKMX010000235         4801 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFKMX010000235 DAFKMX010000000
VERSION     DAFKMX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.240"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            108..1355
                     /locus_tag="LZB08_003429"
     CDS             108..1355
                     /locus_tag="LZB08_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBM7316892.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMSNIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            1442..3436
                     /locus_tag="LZB08_003430"
     CDS             1442..3436
                     /locus_tag="LZB08_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBM7316893.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            3436..4758
                     /locus_tag="LZB08_003431"
     CDS             3436..4758
                     /locus_tag="LZB08_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBM7316894.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDEISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
BASE COUNT         1956 a          466 c          826 g         1553 t
ORIGIN      
        1 taattaaata aattaaaata taattgacaa agtggataca gaatacttaa aatatattta
       61 aataaaatta agcaaaagat agttaattta tgataggagg ggatattatg caaataacga
      121 cttttggagc tatatttggt ttattaatag caattatttt aattataaaa aaatttcaag
      181 ctgtatatag tttaatgtta ggagcattta ttggaggatt agttggagga gcaaatataa
      241 cacaaactgt agattttatg gcaaatggag ctatgaatat atcaccatca attcttagag
      301 ctttggcatc aggtgtatta gctggaagtt taataaaaac aggggcagta gataaaatat
      361 cagaacagat tgtaaaaata tttggtgaaa agagggcttt attttcgatt gccatttcaa
      421 ctatggtact tgctggagta ggtgtaaatt tggatgtatc tataataact gtagcaccta
      481 taggattata tataggtaga aaattaaatt attctaaatt gtcaatacta ttagctatgt
      541 tgggaggggg taaagcaggt aatataatat caccaaatcc aaatactata gcagtagcag
      601 ataatttttc tgtcaatctc tctagtgtta tgatgtcaaa tataattcca gcaattattg
      661 gagttgttat tacagttatt ttagcaagta tattaataaa taaaggaaac aaagtacaat
      721 catatgaaat tttagagcaa agagaagatt taccaagttt atttaaatca ttatgtggtc
      781 caattatagc tatatttcta ttgtttttag gaaatgtatc tcctatagtt atagacccaa
      841 tgatagccct tccaatagga ggaatcgtaa ctctaattgt cacaggaaac ttgaacaatt
      901 caagagaata tttagctttt ggattatcta agatgcaggg ggtatgcata ttattacttg
      961 gaacagggac tatcgcagga attatacaga tgtctgaatt acaacagagt acaataggtg
     1021 ctttacaatt tttaaatatg ccacaatttt tattggcacc agtttcgggt atacttatgt
     1081 cattagcaac agcatcatca acagctggtg ctactatagc atcatctact tttcatgatg
     1141 ctataataaa tggtgggcta tcaccgattt caggtgcatc aatagtaaat gctgggtcta
     1201 gtgtattcga gcaattacca catggctcac tatttcatac tagtgcaggt agtataaaca
     1261 tggatatagg tgaaagattt aagttaattc catatgaagc actgatagga atagtaatga
     1321 ctataatttc aacatctatt caattagttt tgtaataaat ataaatattt aatgaaaaaa
     1381 gtaggatttt gttattaaat attgaattta ttatagtaaa aaagaagatt ggagttttaa
     1441 agtgaataaa aaaaagattg ttattatagg gattatttat tcatttttag tagtattttc
     1501 acttacaaat atgtatgtaa atatggagta taatctaaat gtatttgaat atattaagaa
     1561 gtcacttcca tttacagaag aagaaaaaaa gtggttagaa aaacacaaga atttgattta
     1621 cagctctgac caaagttcac ctcctttgag atataaaggt aaggaagatg gacagtataa
     1681 gggaattatt gttgatttaa taaattctct gtcaattcaa attggtagag atttttattt
     1741 taaaccaaat aattggtgga aagaatcttt cgtaaattca atagatgata gtataaagtt
     1801 ttttgacttg attccatcaa aagaaagggc aaataaattt atatttactg acccaatata
     1861 cactttgagt gcaaatatat tgaaagataa aaagtcccaa gatataaata gttacatgga
     1921 tttaaagggg aaaacagtag caattccaga aggtgattat tctataaatt ttttaaagca
     1981 aaaaattcaa gatataaaca ttttattgac tccagatata aagactggag tcaaccattt
     2041 aatgtctgga aaagtagacg cagttgttgg tgatgaacca gtattgcgat actatataaa
     2101 taattatgga ctttcaaata aatacagtgt tttaagtaat cctatatata ctaaaaaagc
     2161 agttcttgct gtacctaaac aatatgaaga gctggttagt attttaaata aaggcatttt
     2221 taaacttcag aaaaatggag tgtataaaga tttaaagaaa aaatggtatt ctacgtataa
     2281 tgaagttgat gatatattgt atgagcgagg aattgtgcct agtatttatc tttttatagg
     2341 tataatttta atatcaattt atgtttttta tagctataca tatttgttaa agatagaaat
     2401 caaaaggaaa acagaacagg taatagaaaa taaaaaaact ttagaggcaa cttttaatag
     2461 tattacagat ataataatgc ttgtagatga aaataacaat atagtagaat cgaacaaagt
     2521 actatatgac tttatgggtg aaatgagtta taagatagca gatttgatta gcatgataaa
     2581 aggtgttatt gaaaatacct tctctgaaaa tactaataaa actagtgaaa tagaaataca
     2641 caataaaatt ttaaagataa acacttttcc tgtagagtat aaaaaaaata atactgagta
     2701 tatagtagtt cttattaaag atataactaa tgataaaata gtagaggcga aattacttag
     2761 agaaaacaaa atgatatcta taggacaatt agcttctggg gtagcacatg agataagaaa
     2821 tcctttgggt ataataagaa ataattgcta tctcttaaaa gataacgtta caatggaaga
     2881 agtaaatgat tgtgttaaat caatagaaag taatgtagat agggcaagta atataataac
     2941 caacttgtta aatttcgcaa gaatatcaga tgataattta gaacatataa atataagaaa
     3001 ttttattgaa aatatagtga aacttcaata taaaatgtta cagttaaaga atgttgaaat
     3061 aaaaattgac tgtgaacata atttaatatg ctatataaat ggagaatcgt taaaacatgt
     3121 atttataaat ttaatttcta attcaataga tgcaatacat caagatggaa aaattattat
     3181 atactgttat gaaaaaaatc attgtttatt tatagatttt aaagataatg gtgaaggaat
     3241 aaaagaagat gccctaaagg acatatttaa tcccttctat acaacaaaac caattggtga
     3301 gggaactggt ttaggtttat acattactta taatgagatt aaaaagaata atggagatat
     3361 aagtgttgaa agtaaattgg gggttggaac ttgttttcat ataaaaattc cattaaataa
     3421 agaggtgact atataatgaa aatactatta gttgatgatg agctagaata tggagtggta
     3481 atgaaaaaaa ttctacaaaa gaagggctat cttgtagata tagcactaag tggtgaagaa
     3541 gctataaata taattaaaaa agataaaaat tatgatttgg ttttatctga tgttatgatg
     3601 aaaaatatgg atggagtaca gctattagat agaattaaat ctattaatag agatatagag
     3661 gttatattag ttacaggata tggaagtatt gaaaatgcag ttgatgctat gaaaagagga
     3721 gctttatctt attttataaa aagtaatccc attgaaaatt tattagaaga agttgaaaag
     3781 gtaaaggcaa gtaaaatatc tgtttctgta caaaaaaata atctggaatt tactttggaa
     3841 tctaaaaata gagattttaa taatatcata aaaatagcaa aaaaagctgc ttgtaaagat
     3901 gttaatatat taatcttggg ggaatctggt gtaggaaaag atatattagc tagatatata
     3961 catagcataa gtccaagaaa gaatgagata tttgtacctg taaattgttg ttctttttca
     4021 gaaaatctat tggagtctga gctgtttggt catgaaaaag gttcttttac aggagcagta
     4081 gatagtagaa aaggtagatt tgaactttcg aataaaggaa ctctattttt ggatgaaata
     4141 ggagatatac ctctaaatgt tcaggtaaaa cttttgagga cacttgagga caaatctata
     4201 gagaggatag gaagtaataa atctattaaa gttgacttta ggttaatttg tgctatgaat
     4261 aaagaaccaa aagtggaaat atcaaatgga aatataagag aagatttttt ttatagaata
     4321 agtacaataa caataactat accaccacta agaaagagaa gagaagactt aactactctt
     4381 atagaatttt tcttaaataa gtaccaaatt gaacatgata aaaagataca ctctattgat
     4441 aaggaagtaa aggatttttt attaaattac aattatccag gaaatataag agaattaaaa
     4501 aatataataa atagacttgt agttttaagt gaagaaggca atttatcaaa agacaattta
     4561 aatttaatat caaataatgt atacatagat gatgaaataa gtattagacc tttaagagaa
     4621 ataagaaaag agtttgaatg tgagtatatt gaaaaagttt tatctttgtg tggaaataat
     4681 atttcaaata cagctaaaaa attggagatt agtagaagac agttaaccaa taaaatatca
     4741 gagtataata ttaagtgaga aaaaaacttc tcattaagtg agaaaaaaat ttctcaaact
     4801 g
//
LOCUS       DAFKMX010000236         4797 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFKMX010000236 DAFKMX010000000
VERSION     DAFKMX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.241"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            100..780
                     /locus_tag="LZB08_003432"
     CDS             100..780
                     /locus_tag="LZB08_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBM7316895.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            773..2152
                     /locus_tag="LZB08_003433"
     CDS             773..2152
                     /locus_tag="LZB08_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBM7316896.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            2323..3408
                     /locus_tag="LZB08_003434"
     CDS             2323..3408
                     /locus_tag="LZB08_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBM7316897.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMRISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            3462..3821
                     /locus_tag="LZB08_003435"
     CDS             3462..3821
                     /locus_tag="LZB08_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBM7316898.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            3874..4746
                     /locus_tag="LZB08_003436"
     CDS             3874..4746
                     /locus_tag="LZB08_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBM7316899.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKVKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
BASE COUNT         2097 a          418 c          769 g         1513 t
ORIGIN      
        1 tttaatatat aactttgata taataagcat atgtaaatgt ttaatattta attaaagaaa
       61 attatcatat aaaaataaat ttgtaaggag caatacagta tgaaaaaaca atatgtgttt
      121 ataatactag caagtactat tttaggtata cttgttggaa ccatatttag agattatagc
      181 aatgaagaaa cagtttatac aagcaaagac aagtcaataa gaaaagaaat aaaaatgaca
      241 agaaaatcta taaaagaatt aaccaaagag aaagacaagt tagataaaga aattaattca
      301 atgaaacaaa atcaaaaaaa ttctgaagaa atatcagaaa tagaaacact tagagatagt
      361 ctatcatatt cagatgtaaa gggtagtggg ataatattaa gtatagatgc tatagatgaa
      421 aaaacaggga atatagctaa tagtatagac tataataaga ttcttgtaaa agttataaat
      481 gaattaaaag taaacggagg aaaatacata gaaataaatg gtcaaagaat taatcaatat
      541 tcagaagttg tacttgcagg aagccatata aatataaatg gagttcctgt agcacaacca
      601 tatgaaataa agtgcattgg agataaggag aaactatcta attatgtaga taaaggtagt
      661 aaatatttag atagcataca aaccaattat ccaatgaaaa taagcataaa aactgaagaa
      721 aatatctcta tgaaaaaaat gaatatacct aataaattaa agtatataaa gggtgattaa
      781 gattatatta gaggaaagca gtttgccaat gttaatacca gaaaaacttg caaaaaaata
      841 taatgtattt ccagttgagt ttcaagatga taatttagtt gtagaaatag aagatgagga
      901 tatttatgct ctacaggact tgagattagc tacaggtaaa gaaattatat tgaagaagga
      961 gaaaaaagaa cttataagtg aaaagattga aaaatattat aatagctttg aacttgatga
     1021 ggattatgca aaaaaattat ttgaaaatct tttagaaaaa gctataaaag aaaacgcaag
     1081 tgatatacat atagaaccat ttaaagaaca tttaatagtg agaatgaggg tagatggaga
     1141 actaaaggaa gtatcaaagt ttttagttga tgtctatcca tcattgtcta cggttataaa
     1201 attaaaggca tcaatggata taacagaaaa aaggcttcct caggatggta gggtggatat
     1261 aaagttaggt ggaaatttaa ttgacattag agtttcatca ataccaactg tctatggtga
     1321 aaaaatagtt ttgaggatac ttaatagaga tactttttta aaagacaaat tagaacttgg
     1381 tttttcagag gaagctataa aatctataaa tcgtataata aataaaagag caggaatatt
     1441 attagtgaca ggtcctacag gtagtggaaa gactacaaca gtatattcct tattaaatga
     1501 tttaaaagga ataaataaaa atattatgac tatagaaaat cctgtagagt ataagatgga
     1561 tggcataagt cagattcaag taaattcaaa agtagggttg agctttgatg taggtcttag
     1621 agcgatatta agacaagacc cagatataat aatggtcgga gaaataagag atgcagaaac
     1681 agctaagata gcagtaagag cagctataac aggtcattta gttataagta ctttacatac
     1741 taatgatgca gtatcttcca tagctagatt actagaaatg caaataccac catatctttt
     1801 aaatgcctct cttataggtg ttatatctca aaagttggtt agaaaagtct gcaatcattg
     1861 tagccatgag attatgataa aagataattt tagtggagat gtaaatacta aggtagctgt
     1921 agggtgtgaa aaatgtaatg ataaaggata ctttggaaga acagcaattt atgaaatctt
     1981 agaaattaat gatgatataa agacatgtat aagaaatatg gaggattcta gtactattaa
     2041 agatgtggca caaaataatg gaatgataac atttgaagat agttgtaaaa gactaataaa
     2101 tgaaaaaata acgacattag aagagtgttt agtagtaaat gaaatgattt aactgaattg
     2161 tatagttaaa cttcgtttgg tcagttgtct ttatttaatc tattttaatt aagatgtttg
     2221 agtataaatt atatttaagt gaaataaaat taaatgcatt aaattagaaa atattaagtt
     2281 tatttaattg taattaatat aatttggaag gtattatagt acatgagatt atctaaaaat
     2341 atattttata atttagaaaa gttttattta gtattaaata gaatcagtga taaagaaatt
     2401 agagtaattt gcaaagagat aggcatattg ctggaatctg gatgtgaaat tacaaaaata
     2461 tttgaaatca ttgagagtca gtcaagtaaa aaagtaaaga atttgcttag tatagtatca
     2521 aatcatattc aaaaaggtaa ctcaatagca gaatcatttc aaataacagg tatattttct
     2581 aaattcttta taagtatgat aaaagcagga gaaactagtg gaaacttaga tataataatg
     2641 agtgatttat ctaattatta tgataaagaa tacaaattaa aaatgaaaat aataactatt
     2701 tcaatatatc caataatact tataatatta tctatcttat caatgttgtt catattcgta
     2761 tttgtaatac ctaattttca ggtggttttt accaacaatg gaattgaacc acctttaata
     2821 actagagttt taatgagaat atctacagtg gttacaaata acttagctta tataatattt
     2881 agttttatat tgttttcaat aggaagcatt tatttcttta taacaaatga taatataaaa
     2941 aaattgatta attcgttaaa attcaaaata ccttttataa aaaaaattaa tcagttagta
     3001 gctacaacaa gattttcaag ggcattgtcc gttttaatta gcagtggaat acaaattgtg
     3061 gaagctatag acatgtcagc tagtgttata gacaacgaat ttatttatga aaggttaaag
     3121 atttccaata attatataaa aaaaggaaat agcataggta aatctttgaa tttagcaaat
     3181 gtttttccaa aattatttat ttcaatggca aatacaggag aagaaagtgg gaaactagat
     3241 aaaagtctaa atgtaataaa taaattttat gaaaatgaat taaataacaa aatagagcaa
     3301 ataatgaaat ttatagaacc tatgctaatt gtatttatgg gattaattat tggtactttt
     3361 attttagcaa tggttgttcc tatgtttgat gcaattacta atttttagag agaaaaataa
     3421 attttattag atgaaacttt tgaaaaggag agaatattta attgataaat ttgataaata
     3481 aaaaacgaaa aggatttaca cttgttgaaa tgattgtagt agtaactatt ttaggcgtta
     3541 tatctagtat agcattagtt aagtatagta aggttcaaga aagtgccaaa ttaaatgcag
     3601 actatacgaa tgctgctaat atagtaaccg cagctagcat ggcaattaat gatgatgaaa
     3661 agacaataga ctctctaagt gtagagacat tgaaggaaaa gggataccta aatactgttc
     3721 cagttcctca gagtacatca ggtaaattcg aacttgtcat aaatgatagc ggaacagata
     3781 taagcgtaaa tataaattct aagcaatttt atccaaaata atattttata ttgattgctg
     3841 taagttttaa aattattttt atttaagaag gagatggcta taattaaaaa aattctaaac
     3901 ttagaaataa atgatgatta tataaggctt gtatttttaa gaaaaggtca taaaaaagtt
     3961 tttatagata aatcaattat aaaagagatt aactttgata taaaaaatga tagtaattcg
     4021 attgagaagg ttaaatatat agaagatgta gttagatttg taaaagagga gattggtaaa
     4081 tcaaaaatat tatataaaaa tttatacttt aacattcaaa ttcaggatat agtcattaga
     4141 aatgttgaag ttctaaatac taagaaaaaa agagacatat taagcatgat tgaatttgaa
     4201 ataacacaat acattccaat aaacataaat aactattcaa taaaatataa agttataaat
     4261 tctaaaaatg ataaattgaa tatacaagta attcttttcc caaagagtat tattcaatta
     4321 attaagaaca tttcagaaaa tctaaatatg aatccaaaga ctataaatat aaattttgat
     4381 atattacaaa agcttataaa cttaaattta atagagaatt tttcagaaga aggggtattt
     4441 atagaatgta aaaaaagtca gttaattata aatataacaa aagataaaaa aataagagaa
     4501 acatacttgc tatcaaaaga gggaaaatca tatgagttca taaaaagatt attaagggat
     4561 tttaagtatg tttattacta tggtatagaa aattgtttta ttgaagaata ctttaaatat
     4621 acagataaat ctattgaagt aaatccatta agattaaaag ataaagtaaa agtagaaaat
     4681 aattttgatg cagatttaga agacaatagg ataaattata taaatagtat aggaatgatt
     4741 atttaagtga aagtggataa aacaataaat tttcttaatg gatttaaaaa aactaaa
//
LOCUS       DAFKMX010000237         4753 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFKMX010000237 DAFKMX010000000
VERSION     DAFKMX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.242"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..642
                     /locus_tag="LZB08_003437"
     CDS             <1..642
                     /locus_tag="LZB08_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBM7316900.1"
                     /translation="TGATGADGATGPTGATGATGVTGATGPTGPTGATGATGATGASA
                     IIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSM
                     PRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTG
                     ILSVGSISSGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            811..1572
                     /locus_tag="LZB08_003438"
     CDS             811..1572
                     /locus_tag="LZB08_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBM7316901.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1881..2159
                     /locus_tag="LZB08_003439"
     CDS             1881..2159
                     /locus_tag="LZB08_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316902.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2452..3027
                     /locus_tag="LZB08_003440"
     CDS             2452..3027
                     /locus_tag="LZB08_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBM7316903.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3133..3966)
                     /locus_tag="LZB08_003441"
     CDS             complement(3133..3966)
                     /locus_tag="LZB08_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBM7316904.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGTDYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFCNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(4034..4663)
                     /locus_tag="LZB08_003442"
     CDS             complement(4034..4663)
                     /locus_tag="LZB08_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBM7316905.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1760 a          614 c          765 g         1614 t
ORIGIN      
        1 acaggtgcaa caggagcaga tggagcaaca ggtccaacag gagcaacagg ggcaacagga
       61 gttacaggag caacaggccc aacaggccca acaggagcaa caggagcaac aggagcaaca
      121 ggtgctagtg caataatacc ttttgcatca ggtataccac tatcacttac aactatagct
      181 ggaggattag taggtacacc tggatttgtt ggatttggta gttcggctcc aggattaagt
      241 atagttggtg gagtaataga ccttacaaac gcagcaggaa cattgactaa ctttgcattt
      301 tcaatgccaa gagatggaac aataacatct atttcagcat acttcagtac aacagcagca
      361 ctttcacttg ttggttcaac aattacaatt acagcaacac tttaccaatc tactgcacca
      421 aataactcat ttacagctgt accaggagcg acagttacac tagctccacc acttacaggt
      481 atattatcag ttggttcaat ttctagtgga attgtaacag gattaaatat agcagcaaca
      541 gcacaaactc gattcttact agtatttact gcaacagctt caggtctttc attagttaat
      601 actgtagcag gatatgcaag tgcaggaatt gcaataaatt agattagata agaaaaggct
      661 tctaatatcg tattgatatt aggagctttt ttaagaatga ttgatgaaca tagttttaat
      721 attaattagt gcgcatatta taaaatacta ttatataata taaaataata acattaaatt
      781 ggaaatatag aattatctag gggtgatact atgaaaaata agattgaaat atcaatgaac
      841 aactttgtaa aaaattatca taaatcaaat aatataacta caaagtggca agaacttttg
      901 tttgcttatg catctgctga agatgatatg tttcagaagc taaaaagtgt tgtaggaaca
      961 tctcatgcta tgccaaaaga ttttctggaa aatggaaaaa ctataattac atattttata
     1021 ccttttgatg aaagtgtaaa taatagtaat attggagggt ttgaatgttc tgaaatatgg
     1081 gcaagagcgt acatagaaac taatatgttg atttcaaatt taaatgattt cttatgtgaa
     1141 gaactagaga agataggggc taattcatat gctattcctg ggtcgtataa ttttgacagt
     1201 gaaaaattgg taagtgactg gtcacaaaga catgtagcat tcatagcagg tcttggaact
     1261 tttggtttaa ataatatgct tattacagaa aaagggtgtt gtggaagaat aggaagcata
     1321 atcacaagtt tagaattaga gccaactaaa agaccagaaa atgaatactg cttgtataaa
     1381 cataataata gttgcaaaaa atgtgtcaat aaatgcgtga aagaagcttt aaaagaagat
     1441 ggatttaata ggaataagtg ttatgaaatg tgtatgataa atgaaaaaaa atacacttat
     1501 ataggtggat gtgatatttg tggaaagtgt atagttggag ttccttgttc agtaataaac
     1561 ccatcaagat aatggattta agttgactaa taattttaaa gatagtttat aatgaaaaaa
     1621 ttttagttat caaaataaat ataatataaa aactaaataa atgtgtttaa aaaatggata
     1681 aataatttgt gttgattgac aaccagttat actgttgata gacttatatg tatcgctgtg
     1741 atatataagg agggttgaga ttttatgttt gatttaatct ttatttttgc atagcaaggg
     1801 ctattgcaac tatgaaattt aatatataaa tagccctttg ctattcctaa tgaaaaatta
     1861 ctatttaata ggagagcaaa atgagctata aaaaagcaat acatatatta ccagaagaat
     1921 tacttgaatt aatacaggaa tatgtagatg gtgaatgtat ctatattcct agaaaatcta
     1981 ataataaaaa agaatgggga agtaatacac ctactcgtga agagttgtca attagaaata
     2041 tgcagattta cgaagactat caagttggat atgatttgca atatttatcg gaaaagtatt
     2101 atttatcttt gaaaagtatc caaagaattg tattacaaga aaaaagaaaa gatatctaag
     2161 gatgagccag tatagtttta taattatact ggctcatttt cttttgataa aagtgtttga
     2221 ggtatagttt cttttaagtg gatgatataa tttaagtaat ttcaataact tatacagtag
     2281 ctaacaaagc tggttgtaaa gtatctaata agaagtagta gctttttata tgtttgaaat
     2341 taaaatttat taaaattagg aggtcttgtt ttgaaaaaaa tacttgatta tattccacct
     2401 tttcataact ttctattaat ttagtaatct tatagaaagg aaattgcatt tatgaaaaaa
     2461 attacttggg aggtggaata tatctcccag ttacctgtaa ttaggtattg taaaaaatgt
     2521 ggaaaaaaga ctgaatatat ttgttcaggt ttatttagag ttaatgctca acataaatat
     2581 ttggatatat ggttaatata caagtgttca aattgtgatt caacatggaa ctcaactata
     2641 tattctcgtg ttaatccaaa aagcttaaat ccagaaatac tagaacaatt ccatacaaac
     2701 gattataatc ttgttaagca atatgctatg aatatggaac ttttacatag aaatggagca
     2761 gaagttggta ttccagagta taaaatagta ggacaagaaa ttaatattaa tgaccttaca
     2821 caagttcata ttattagtaa atatccatgt cagttaaagg tatcaacatt attacgtgag
     2881 aaactaggtt tatctaggaa ggtatttgac gaaatgttgg attgtgggat tattagaagt
     2941 aactcaggag ttgatttaaa gaaaggtaaa ttaaattatg aaattatgtt agatataggc
     3001 aaggagaagc aaaagtcttc aaattgatta gaataactaa cttaatatat tttcaggaac
     3061 atcatttaaa aaaatagtga ctttaaaaaa tttaaagcca ctatttttta atatgtttta
     3121 tctatataac tattattttt ctaggttttt taatatttcg taatagtgtg ttgtggctat
     3181 ttcacttgac tcgccttttt tacaaaaaga taatttagat agttttggaa ctacacgttc
     3241 aacgacaatt ttattaccta tcattttaga ttcaagtatc ttcaatgctt ctaaaaatct
     3301 cttatccttt tttgcacgat tataaaatga taaaacgtag acataaagga ataggttata
     3361 attacagaaa ggatattcaa cttgcataaa taatgtgcca attccatagt gacaagggcc
     3421 gagtggtttt ttaatagtcc aatggtcaag tagaaaatct acagctttgt caagttttga
     3481 ttctttattt agataattac taaaacggaa tgcatttaat atattaagag ttggtagtgg
     3541 atttgagtat tcagtttctg gacctcgacc aaaactaaat ttattgcaac gccacccacc
     3601 atcagtatac tgtgtatcta aaaaatgttg aaacgttttt tgcattctaa tgtcagatgc
     3661 atatcccata tgacagagta cgttagcagc atttgcagta tgacaagggt aaatacctcc
     3721 atttggatat agtttgaaac gtccatcttc tcgccatgtg ctaaatatca agtcagcaca
     3781 ttctttaagt aaagtatcat cagggttcac acctaactct aatagataaa gtacactatc
     3841 aagagtagag aaaggagaac cttttattaa acgcttatca ggtgtagtcc aatagtctgt
     3901 accattatca tacctgtgtg ataatattat ttcaatatct gataaagatt gattttctac
     3961 attcattttt tactccattt catattatat tctattaaat ctatcaaaaa ctttattata
     4021 atattattta tatttataat tgatttattt tatttaaaat tgcatttata gctgtatcag
     4081 gaacaactgc taaatcttct gcattgaagt aataaactat attagaatca aataatatat
     4141 ttgaatttgg aggaaattca tcatctccaa accaaagtat aaataccata gatgtattac
     4201 ccatatatgt aaacctataa gctaaatctc ccatgtttac tttttcagct ccaattttct
     4261 ccataactaa cttatatttg tctatttgag atgtaaaaac ttctgaaagc ctatatagag
     4321 tcctgtttaa aaaatttgga taataaacat tgcctcctgg tatttcttta aaagttacat
     4381 atttattagt tctaggaata cctttagcat tgattaaata tctaactatc aagattttaa
     4441 gctcaaagtt attgcaaagg ctaccattac tattccaaac ttctccactt ggatagtcta
     4501 taatatattg tctatcaaaa aaattgagtg tgaatctatt taaatcatta ttgaaaggta
     4561 tattacttaa attactaatt gtatgagggt ctaattcttt gagttttttt cgaacatagt
     4621 caaatggaac attataatta ttttttgtta tcttttcatc cataattttt tcctccaatt
     4681 taaacaatta aaagtatatt tataaaaagt tttcttatta ttaattttat tatatattca
     4741 aaatattaca ata
//
LOCUS       DAFKMX010000238         4655 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFKMX010000238 DAFKMX010000000
VERSION     DAFKMX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.243"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            106..795
                     /locus_tag="LZB08_003443"
     CDS             106..795
                     /locus_tag="LZB08_003443"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBM7316906.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            796..1368
                     /gene="yqeK"
                     /locus_tag="LZB08_003444"
     CDS             796..1368
                     /gene="yqeK"
                     /locus_tag="LZB08_003444"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBM7316907.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            1485..1835
                     /gene="rsfS"
                     /locus_tag="LZB08_003445"
     CDS             1485..1835
                     /gene="rsfS"
                     /locus_tag="LZB08_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBM7316908.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            2189..4609
                     /locus_tag="LZB08_003446"
     CDS             2189..4609
                     /locus_tag="LZB08_003446"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBM7316909.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDALEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
BASE COUNT         1884 a          514 c          896 g         1361 t
ORIGIN      
        1 ttttagcata taaatataat tatataccca aaaaaacaaa ttataagctt taagaaataa
       61 aaaataatgg tacaatagta ttataacatt aaaaggagac aaaatatgag aagtgaaaat
      121 ctagttaaga tggcagagtt gaaaaatact gaaaaactag agaaatttaa tcgccataaa
      181 ggcaaaatca aaataggtat attgggaggt acttttgacc ctatacacta tgctcattta
      241 gcaacagcag aatttattag agacaagtat gacattgaca aaataatctt tataccatca
      301 ggaaatccac cacataaatt atgtatcact acagataagt atgatagata taatatgaca
      361 cttctagcaa ctgaaagcaa cgaagatttt ttagtttcaa aagtagaaat cgaaagaaag
      421 aaaaggactt atacaataga tacattaaaa tacttaaaga aaaaatacaa aaatgcagac
      481 atttatttta taacaggtgc agatgcaatt tgtagtgttg aagagtggaa agatgtaaag
      541 aaaaactttg agttagctac ttttatagcc gcaactagac caggaattag tttgttaagg
      601 tcacaagaga ctatagaaaa actaactaag aagtataatg ctgacattat aacagtttat
      661 gttccatcac tggatatatc gtccacatat ataagggaac agctaaatga aggcaagtca
      721 atacgttact tagtacccga aaatgtagaa aattatttat atgaaaataa actatatcaa
      781 tatggagatg attaaatgaa cttagaaaat attaaccaaa gactaaatca aatgctacca
      841 gtggggaggc ttagccattc aaaaaatgtt gcaaaatgtg ctgaaaaatt atgtgagatt
      901 tatggatgtg acaaagaaaa agcttactta gcaggaatga ttcatgattg tgcaaagtat
      961 ttaagtgaca aggaaataga ggattatgta aataagtatg aaatatattt agacccaata
     1021 gaggatggaa atcgttcctt gtctcatagt gtaattgggg cgtacatatg cgagtatgaa
     1081 tttgaagtag aagatgaaga tattataaat gcaatcaaat atcatacaac agggagagag
     1141 gacatgtctc ttttagaaaa aataatttac atagctgatt taattgaaga aggaagaaaa
     1201 ttcccagttg tagatacatt aagagaatta gcatatggag ggaagcttga tgaagcactt
     1261 ttaacttctt ttaataatac tttaatgttt gtcatcaata aaaaagaaga gatacatcca
     1321 agaacagtta tggcaagaaa ctatctaatt aaagaaaagt tattataaaa aagctatata
     1381 aaggcaaaca tattaataaa tttaaatttg actatgttac aaaatcgtat taatatgtta
     1441 caataactaa gtgtatttta attaggaaag tgaggaattt ttatatgaca gtggaacaaa
     1501 tgacaaaaat agcatatgat gccatagaag ataaactggg tcaagataca gttataataa
     1561 atataggaaa agtttctagt ttgtgtgatt attttattat tactacagca tcttctcaga
     1621 gacaggtaaa agctattgct gacaatgtgg aagatgaatt agcaaaacta ggattagagc
     1681 caagaggtaa agaaggacaa ggaactcaaa cttgggtact ccttgattat ggagacataa
     1741 tggttcatgt atttaatgag gaaaatagag ggttttataa tttagaaaaa ttatggaaag
     1801 atgcacctta tatagatatt gacacattag cataaacatg ataatatatg ttattataat
     1861 tagaaaattt caaaaattat attaaattga atagactaga gcagtaaaag attatatttt
     1921 ttcagagagt tagtggaagg tgagaactaa tattatataa ctttgaactt actagattaa
     1981 aatctattct ccatggctgg attaaaagct atctaagaga gtctatatat gtagtaggct
     2041 aattagggtg gtaccgcgaa gatttatcct cgtccctaaa cgtaagttta gtgacgagga
     2101 ttttttattt tcaaagaatt atttggaaat tataagtaaa taatactaaa taaggtagtt
     2161 atacacaatt aaaaatagga ggcaaaatat gagcgtatat aattttaaag aagtagaatc
     2221 gaaatggcaa aaaatttgga aagataataa tcagtataaa atggatactg cacaaactga
     2281 aaaacctaat tattacacat tagaaatgtt cccttatcca tctggaaaaa tacacatggg
     2341 acatgttaga aattattcta taggtgatgt tgtcgcaaga ttcaaaaaaa tggaaggcta
     2401 taatgtactt catccaatgg gatgggattc ttttggatta ccagctgaaa atgcagctat
     2461 aaaacatgga atacatccac ataaatggac aatggaaaat atagaagaaa tgaaagagca
     2521 attaaattta ttaggtatta gttatgattg ggataaagaa gttgcaactt caacgccaga
     2581 atactataga tttacacaag aaatattttt gaagttttta gagcatgggc ttgcatataa
     2641 gaaaaaatca tatgttaact ggtgtccttc ttgtgagact gttcttgcaa atgagcaagt
     2701 tgttcaagga gcatgtgaga gatgcaaagc aacagtatta aagaaagatt tagaacaatg
     2761 gtattttaag actactgagt ttgcagaaga attacttaat gacttagata cattagatgg
     2821 atggccagaa aaggttaaaa caatgcaaaa aaattggata ggtaaaagta caggagcaga
     2881 tttagttttt gatatagatg gaactgataa atctatgaca gtatttacaa ctagaccaga
     2941 cacaacttat ggagttactt atatggtact tgctccagag catgaattag ttaaagaatt
     3001 ggttgcaggg actgaatatg aagcagatgt tgaagctttt gttcaaaaaa tgcatactat
     3061 gacagaaata gaaagaacag cagctgatgt agaaaaagaa ggaatgttta taggtagata
     3121 tgttataaat cctttaaatg gcaagaaagt tcctctatgg atagctaact atgtattagt
     3181 tgagtatgga acaggtgcta taatggcagt tccagcacat gatgaaagag atagagattt
     3241 tgctgaaaaa tataatttag acataattga tgtaataaca gaagataaca aaatgataaa
     3301 ttcagaagaa tttaatggat tagatgcttt agaaggattt gaaggaataa tcgataagtt
     3361 agaaaaagaa ggtagaggaa agagaacaat taattataga cttagagact ggttagtttc
     3421 aagacaaaga tactggggtt gtcctatacc tgtggtttat tgtgatgaat gtggaatagt
     3481 tccagttaaa aaggaagatt taccagttct tttaccaaca gatgttgaat ttactggaaa
     3541 aggggaatct ccacttacga cttcaaagca atttatgtca acaacttgtc ctcactgtgg
     3601 aaaaccagct agaagggaag ttgatactat ggatacattc gttgattctt cttggtattt
     3661 cttaagatat gtggatagca ataatgaaaa tgaaccattt agtaaggaat tagttaatag
     3721 atggcatcca gtagaccaat atataggtgg agtagaacat gctataatgc atttgcttta
     3781 tgcaagatgg tttgtaaagg catttaagag tatgggaatg gtagacttca atgagccatt
     3841 taaaaactta cttactcaag gaatggtact tatggatggt tctaaaatga gtaagtctaa
     3901 agggaataca gtatctccta tggatataat agatgagtat ggagcagata ctgcaagact
     3961 tttcgtatta tttgcagcac caccagaaag agatttagac tggtcagagc aaggtgttga
     4021 tggatgcttt agattcttaa atagggttta tagattggtt gacgagttag cagatgtggt
     4081 taaaaaggat gtagagtttg gagaattaaa ttcccaagat aaggatatga gatatactat
     4141 acactctaca cttaaaaaag ttactgctga tttaagtgaa aaattcggat ttaatactgc
     4201 tatatcagct cttatggaat taataaatga tatgtataaa tataaagagt tagataatat
     4261 aaacgaagca gttataaaag aaggtgtaca aactatagta actataattg caccatttgc
     4321 ccctcattta ggtgaagaac tatggactat gataggtaaa gaaggaagtg tatttgatat
     4381 agactggcct aaatatgacg aaaaagcttt agtaaaagat gaaatagaag ttgttgtaca
     4441 agttaatgga aaagttagag gaaaattaac tgttaactca aatatatcta aagatgagat
     4501 ggaaaaggta gctttagaag atgaaaaaat aaaaggatta gttgaaggta aaactatagt
     4561 taaagttgta gctgtaccta aaaaattagt aaatatagtt gttaaataac tcaaaaatat
     4621 aaacaaaaat ataaatttat aaaataaatg aggct
//
LOCUS       DAFKMX010000239         4609 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFKMX010000239 DAFKMX010000000
VERSION     DAFKMX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.244"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            151..1119
                     /locus_tag="LZB08_003447"
     CDS             151..1119
                     /locus_tag="LZB08_003447"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBM7316910.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1236..2366)
                     /locus_tag="LZB08_003448"
     CDS             complement(1236..2366)
                     /locus_tag="LZB08_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316911.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2519..3472)
                     /gene="msrA"
                     /locus_tag="LZB08_003449"
     CDS             complement(2519..3472)
                     /gene="msrA"
                     /locus_tag="LZB08_003449"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBM7316912.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYNGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3596..4501)
                     /locus_tag="LZB08_003450"
     CDS             complement(3596..4501)
                     /locus_tag="LZB08_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBM7316913.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFRLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         1536 a          753 c          531 g         1789 t
ORIGIN      
        1 agtatattaa cattgctttt ttaaaaattt tagtataaaa tcatattaaa taaattttct
       61 gaaataatag aatattaaaa tatccaaaaa cagcttttaa taacatctat aattttcaat
      121 gtatcgttat tttaggggga gtgatttaaa atggcaataa agccaagaaa aatatctatt
      181 gtaggttcag ggcatgttgg ttctcattgt ggatttagtt taataactca aggagtttgt
      241 gatgaactct ttatgattga tatagatgaa tctaaatcaa aagctcaagc tttagattta
      301 gctgatgcag tttcatatct gccacataaa gtacatatag aaaaaggaac tttttctgac
      361 tgtaaagatt ctgacatagt agtgataagt gttgcagact cttcagaagg gcctttacgt
      421 agacaaaaca caactagatt agatttacta agaccaacta ttggaatgat aaaatctata
      481 gtaaaaccca tagttgattc tggctttgat ggtatatttg tagttatatc taatcctgtt
      541 gatgtagtta caaattatat ttgggaaaaa tctggctttc ctaaaaacaa agtcattgga
      601 actggaacag ctttagattc aacaagactt agaaggattt tatctgaaga aactggaatc
      661 gctcaacaat caattcaagc ttattctatg ggtgaacatg gagattctca aatggttcct
      721 tggtctcatg tttcaattgg tggtaaacca atcttagaca tgataaaaga caacccaagt
      781 acttatagca atttagactt accatcaata gttgaaaaaa ataaaaaaac aggaataagt
      841 ataataaatg gtaaagattg tacagaattt ggtataggta ctgctttagt agaaattgta
      901 aaagctatac tacacaatga gaaaaaagtt cttccagttt ctacattact agaaggtcaa
      961 tataacgaaa gaaatgtatt tgcaggtgtt ccatgtgtta taggaaaaga tggtattgaa
     1021 gaaattattg aaatcaatat gactgaatat gagcaaaacg aatttaataa atcttgttct
     1081 gttttaaggg aatatattgc actttcaaaa acactttaga ttcaatattc tttcttacac
     1141 taaaagcagg taaatgactt attgaaaaga cttttaacta agttaatgtt taaatcattt
     1201 gtacctgctt ttaattttaa tactaaaata taaacctatt ttttaccatc aaattttata
     1261 actttacttt ttttatttac ctcttgagat aacaatcctt ttttcaatcc catcaataat
     1321 tctaagtctt ctggataaag atttattcga aattcctcta aacttgatac tatactattt
     1381 acactcatta ttttattttc tatcaataat tcaactgctc tttttaaata cgttgggctt
     1441 gaacctttca ttttgtctag tggttcttct atcaaccagc cttttttatc catttcattc
     1501 ataagataat tgtatttttt gtaatttatc ttttctagat tatatgctct aaaaatcata
     1561 gcagatatag gtacaatcca ttttttcttt agttctacat aataatctaa atcttctgga
     1621 tgagtcaaat cttttaaaaa actctctttt ggcaataaaa atgcacaggc atactcatct
     1681 tctgaaaatt gcttacttgg tattcttaat tcacttgata ttatatatcc aagttcacat
     1741 gccaaagtat aatttctaat tgatgctgac tttttgtcat tacccagaga tattagatat
     1801 ttagattctt tactaattct ctgtttttga gtaaagatag ttgccccttt tctatctaca
     1861 ttcatacctg atattataat ccctttagac tctaacaaac ttaccatatt tactattgga
     1921 gtactattta aatcccaaaa ctctctagtc ttatatgcaa gagattctat atcatttata
     1981 ttaatatctg acattacttt tctatttggc aagttttctt ttggaaactt aatataattc
     2041 tccataaatg aatatatttt atgtattata attatttttt ctctataaga aatctcttct
     2101 actctaggta gtttagattg agggttaaag tggctatcct ctacaacgat atttatattt
     2161 tcatttttat aaaaatactc ttttggaaaa tttaatatat tagataattt taatgtattt
     2221 tctatatttg gtacatactt attttcctca aaagctttta aatctttttt atttatttta
     2281 gtttctttag acaactgttc tatcgtcttt gctctaaaaa ttctagctga ttttaatctc
     2341 tttccattaa agtcttttaa attcatttgt tttctctctt tcaaaattaa attcttaatc
     2401 tattggtata aataagatta caattaataa tttatcatac tattgacaaa aaaacaaaag
     2461 gtgaaaatat ttttttagat tttcaccttt tagataagtt tataataaat attcctaatt
     2521 actcaaaaag ttttaaatat tctccatatc ctaaagcttc catattttct cttggtataa
     2581 atttaagaga agcagaattt atacaatatc ttaaacctcc aagttctttt ggaccatcat
     2641 caaatacatg acctaaatga gaatttcctt tagcacttct tacttcaact ctgtacatac
     2701 cttgtgagaa atcacttctc tcaatgatag atttatctat aactggcttt gaaaaacttg
     2761 gccacccaca tccagaatca aatttatctt ttgaagaaaa taacacttct ccacttacaa
     2821 tatcaacata aattccctct ttgttattgt cataatattc attttcaaaa ggtacttctg
     2881 tcatatcatt ttgagtaact tcaaattgta taggagttaa agtctttttt aactcttccc
     2941 tatttatatt gtttccatcc caatgacttt ttacaaaatt atatcttccg gaccctttat
     3001 aatacatatt gtagtttaat ggtcttttct tataataatc ctgatgattt tcttctgctt
     3061 cgtagaattt agttgctgga attatatcag tcactatttt actatcaaat attttactat
     3121 tttccagatg ttctcttgac tctaaagcta tatttttttg ttcttcatca tgataaaata
     3181 tagcagtctt atattgagtt cccctatcac caaattgtcc accatcatct gtaggattaa
     3241 tctgtttcca gaagatattt agaagttcat cataacttat tatatcatca tcatatgtta
     3301 tttgaattgc ttctaaatga ccagtagttc ctgaacatac ctcttgatat gtagggtttt
     3361 caacatgacc acctgtatat ccagaaacta ctttaataac tccattatac ttatcaaaag
     3421 gttttaccat acaccaaaaa catccacctg caaaagtagc aagttttatc attttcatca
     3481 ctccttaaat aaattatact ttatttattc cctaagaagt aacttaataa tctgcatttt
     3541 attattttta cctactcaat gaaatcatgt gtaaaaatac aattgcaaat tgaatctaat
     3601 ttctataaaa gctctttttc tttatccatc caaaagcatc ttttatatca caattttctc
     3661 ctaaagacat tagatattct aatgcatcac aatcatccat agctacatct ttttccatct
     3721 taggatttaa tctatagtaa ttatctccaa gaagtttttg agaaaataac tgactcattt
     3781 gagaatttcc ttctaaggta atagatataa ttggtaaatc gggttcttta ttaattatcc
     3841 agtcaatagc tccccattcg gtagtatctg cctttatact gttatataca tatccagtcc
     3901 ctattgaaaa aagtcttgta ttcttaagtc taaatccact gtcaatggca tgaattatac
     3961 acgctaaact cgggtcagtt gctattattc ctccatcaac atgacgatta tatgttggaa
     4021 aaaataccgg agcagcacta cttgccattg ctacatctac aactttgcta gtctttgtaa
     4081 aagaatttgg catattgtta taaaatactg gtttccatgc attttcctca tttcctatat
     4141 aaaaacttgg tatcattact aatttattta aatcttttaa ttctagttcc tctggaaata
     4201 tacttaataa aacttctttt aagtttttgt tttcatactt tggtctgagt atttctgcat
     4261 aacttttatt aaaaatatac tttgaatttt caattgagta aagttctttt atctctttag
     4321 ggcttacacc atacgcaagc cctagtgcaa ttagactccc tgtagatgta cctccaaaca
     4381 tatttatatt gtttaataga tttggatact cttttactat cctctcaagt atggatatac
     4441 tcaatgcccc ttttaatcca ccaccatcaa atgccaatat attaaaatta gaatttttca
     4501 tatacatata cccccacata aaacacctta tattttaaat tttaatcaaa ataatttcac
     4561 tatagtatat atattttatg caagttacat attacaatgt tcctctttt
//
LOCUS       DAFKMX010000240         4518 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFKMX010000240 DAFKMX010000000
VERSION     DAFKMX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.245"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(53..568)
                     /locus_tag="LZB08_003451"
     CDS             complement(53..568)
                     /locus_tag="LZB08_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBM7316914.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            848..1375
                     /locus_tag="LZB08_003452"
     CDS             848..1375
                     /locus_tag="LZB08_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7316915.1"
                     /translation="MHEQIDCCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            1359..2762
                     /locus_tag="LZB08_003453"
     CDS             1359..2762
                     /locus_tag="LZB08_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7316916.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(3416..3727)
                     /locus_tag="LZB08_003454"
     CDS             complement(3416..3727)
                     /locus_tag="LZB08_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316917.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(3783..4376)
                     /locus_tag="LZB08_003455"
     CDS             complement(3783..4376)
                     /locus_tag="LZB08_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBM7316918.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         1699 a          534 c          681 g         1604 t
ORIGIN      
        1 ttatgaaaag ataatcagaa atttagtaag tttttataaa aaaaattata tcttatagat
       61 ttttcagtaa atctttacac caattaataa caaaattata atagtcaact ccataaatca
      121 ttgttgcata catatatgta tattggtcaa cacaatgatt ttcttctgca tcattttttt
      181 gtttacttaa tttatttaga agtaattcta cttcagttat taacattttt aagtttgttt
      241 caattatttc tttgggtaga tacttgtaaa agaatatatt aacaaggtgg tcttgctttg
      301 ttttagaaaa atttataggt ttctttaacc attcaagaaa tacttcttta ccagtattag
      361 ttatggaata tagttttttt aatttgttac catctataac ttcgtgatag tgtatatatc
      421 cttttgtttc taatctttta agtgcaggat agatacttcc aaagctggcg tcaaaaaagt
      481 acgaggtact ttctgacatt atttgtttta agtcgtatcc acttaattct ttttccatta
      541 aaaatcctaa aataatgtat tctaacatat tacctccttg tatcaattag atatatatcg
      601 attcgatata tttattatta cagataataa atcttacgtc aagatgcttt ttaaaataag
      661 ctaaaaagaa ttaggagtta ttgataaata tgcaaaaaaa ttaatataaa aggtaattaa
      721 atatcaatga agatttaaaa atagttaaat caaagtaaga gtctaatata taaaatttat
      781 agtaacttta tggtttaaat ataaacttgt gtgctattga caaagtataa aattagcagt
      841 aaaataattg catgagcaaa tagattgttg cgaacgcaaa aaaataagga caatagatat
      901 taaaggagag aatatgagag agctattaaa atgggtttcc attataaata gatacaatac
      961 aaattatatt gacaggaaat tggcggatat tggaatcaat aatagccaat atttttatgt
     1021 attacacata tgtaataatc caggtattac acaggacact atatttgaga aaattcttgt
     1081 gaatcctagt aatataacaa gagctttggc aaatttagaa aaagaaggct ttataacaag
     1141 agaaccaggt gtaaaggata aaagaacatg gcatttatat cctacagaaa aatctttagg
     1201 atgttatgat gaaatactaa aaataacaaa tgggtatgtt gaggagttgt tgagttcatt
     1261 tgagaaagaa gagcaagagt tgtttatgtc aatgttaaag aaagttgcat ttttggctat
     1321 tgatatgaat gaagaagcaa aattggaggc gaaggaaaat ggaagaaaaa tataaggagc
     1381 cattggaata tgaaaaggtt tcaaagttat taactaagta cgcaattcct agtattatag
     1441 caatgttagt aagtgcttta tataacattg ttgaccaaat atttattggt caaggtgttg
     1501 gtgtattggg taatgctgct acaaatgtca cttttccact aacaacaata tgtactgcaa
     1561 ttgcattatt gttaggaatt ggaggagcgt ctaagtgttc attggaatta ggtgctaaaa
     1621 attcagaaaa agctacaaaa gcagcaggaa attctatatg cttgatggct gtttttggaa
     1681 taagtttatt tatagtagta tcaatatttt taacaccact attaaaattc tttggctcta
     1741 catcagaaat actaccttat gcacacacat atacaaaaat tacatcaatt ggattaccat
     1801 ttcttataat gtccactgga atgagtaagt tgatacttgc agatgggaga ccaaaagctt
     1861 ctatgttttg tatgttaata ggagcagtta ttaatacaat tttgaatcca ttattcattt
     1921 ttgtttttaa tatgggaatt gctgggtctg ctttagcaac tgttattggg caacttatat
     1981 cttttggaat aagtgttcat tatataaaga attttcaaca tattaaatta cataagaaaa
     2041 gttttaaatt agatagtata atttataaac atatatttgc gctaggttca agctcatgtt
     2101 ttaatcaatt agcaatgact gtaacacaga tagttatgaa taatacattg gcatattatg
     2161 gggctaggtc agtttatgga agtgagattc cacttgcttg tgtcggtatc attattaaag
     2221 taaatatgat ttttatatct ataattattg gtatatcaca aggtatgcaa ccaattatag
     2281 ggtataatta tggggctgaa aagtataatc gtgtaaaaga agcatataag tttgcagtag
     2341 gaagtgcaac tattatatct gttattgcat tcttatgttt ccaattcttc ccaagacaaa
     2401 ttacgtctat atttggtaac ggaaatgaga cttattttat atttgcagaa cattatttta
     2461 gaactttctt gttcttaaca tttataaatg gtgttcaaat agtatcatca actttcttta
     2521 catcaattgg gaaatcagtg ttgggacttt ttacttcttt gactcgtcaa attttatttt
     2581 taattccatt gatagtaatt ttaccaatgt ttatgggaat tgatggagta atgtttgcag
     2641 gaccaatagc agatgcagca gcggcaatgg tatgtatata ctttgcagtt catgaaatga
     2701 aatcattgac tataaagcaa aataatttga tgagtaacca taataagatt gcagaggtat
     2761 aagacaatat ttaagaagta tagaaaatta atttaaagaa gtataataaa ctaatttagg
     2821 aagtgtagaa aattaatcta aagaagtata ataaactaat ttagaaagta tagaaaatta
     2881 atctaaagaa gtgtaataaa ctaatttagg aagtatagaa aattaatcta aagaagtgta
     2941 ataaactaat ttaggaagta tagaaaacta atctaaagaa gtgtaataaa ctaatttagg
     3001 aagtatagaa agctaatcta aagaagtata gtaaactaat ttaggaagta tagaaaatta
     3061 atctaaagaa gtataataaa ctaatttaag aaatatagaa aattaattta aagaagtata
     3121 ataaactaat ttaggaagta tagaaagcta atctaaagaa gtataataaa ctaatttagg
     3181 aagtatagaa aattaatcta aagaagtata ataaactaat ttaagaaata tagaaaatta
     3241 atttaaagaa atacaataca ttaattttta agtaacatta aattaatgta agtaaaataa
     3301 tgttcatatg aagatttaaa ggtgcatttt ctgaagctaa gtatatgttg tagagtgtta
     3361 tgtctctcta tccggtgaag tttaatagct caagaaaatg cactatttat gatgattatt
     3421 ttgttttatt tccaattttg gaaataacta ttctaattaa atctaaaatt ataccataaa
     3481 taagaaaaga aagtatagat gctatgtacc aataaatcga aattgaccct gtaattgttc
     3541 tacatgggat ttgcatacct gaatccataa aaacaaaaaa actatcaact ctggaaataa
     3601 tagacaaaat aggatttaaa cctattagaa caatactttt tgagtcttga ccaatttgat
     3661 gcatataaat ctcaaataat gcaattaaaa aaaaccaaaa tgtaaaagat ttaataattt
     3721 ttttcatcaa aatacctcac ataatataaa ttctgtttgc actatttgct tactacaact
     3781 atttatctcc attcagtcac atcacttaat tgtaatctag gtcttcttga tggttcttca
     3841 tcaggataac ccaaagatat tgctgccact aattgactat ctgtatttaa ccattcacat
     3901 aattcacgat atgcaaagta tacatcacaa atccaaagac tacctagacc taattccaca
     3961 gctgtaagcg acatattctg aattgcagct ccaattgatt gcatgtttgc catttcatag
     4021 aatcgttctt caggggatag cttttctaaa ggtgatttac ctaatatatt taaaataaaa
     4081 attgttactg gagcttgttt catgatttca actgtgtaat ttgcaccagc gatatgttga
     4141 cgacttcctg gaagaagccc attatcatta atttcatttt gaataccttt gctcatggct
     4201 ttaagcatag attctttttc cttttcagtg atgacaacaa aacgccatgg ttgcctgttt
     4261 ttagatgaag gtgcatttat tccagcttca attattttct ctatagtttc atgtgaaatg
     4321 ctttgatttt tatattttct gatacttcgt ctttttgata tagaatcact tatcatttta
     4381 agtaacctcc gtttattttt tggaaaaatc tatttaattt ttattttcta ttttaattat
     4441 tttagcatta atcagaaaaa aagtcattga ttttagtata caattattaa tgattatatt
     4501 aagaattgta tcaaaaag
//
LOCUS       DAFKMX010000241         4510 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFKMX010000241 DAFKMX010000000
VERSION     DAFKMX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.246"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            78..377
                     /locus_tag="LZB08_003456"
     CDS             78..377
                     /locus_tag="LZB08_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316919.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            701..2356
                     /gene="ilvD"
                     /locus_tag="LZB08_003457"
     CDS             701..2356
                     /gene="ilvD"
                     /locus_tag="LZB08_003457"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBM7316920.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELETLAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGIVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2700..3329
                     /locus_tag="LZB08_003458"
     CDS             2700..3329
                     /locus_tag="LZB08_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7316921.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTFTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3355..4167
                     /locus_tag="LZB08_003459"
     CDS             3355..4167
                     /locus_tag="LZB08_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7316922.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELSKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1749 a          501 c          815 g         1445 t
ORIGIN      
        1 aaattttatc gtatgtataa atattataaa tatatggaaa taataatagt atattttggt
       61 aaagtaggag gaattttatg aaatggatta aatggtatag tattacatgc atatgtattt
      121 ttatagtagt agctttttat atgtttatat ttcctaataa aatagaaact attgatactt
      181 catctgcata tagctttgtt gaaaagaaag taccaaatag tgctgtgtat caaggataca
      241 aaaagaatcc agttgatggt actactacaa tttattacag ctatgataat tcaactcata
      301 tagttagatt atcacatcca gaagactatt caaggaagat taattgggat aaagtttcaa
      361 acatcagatt tgattaatct ttaatattta taaaagtcta tatatctaag gcaaatatat
      421 tattaatctt attcatagct gagcactttc tattatagaa gtgcctcttc tgatttatat
      481 cataaaatat tactcaacaa tttgtaaaat atattagttt ataaaatata ttgaaaagat
      541 aatattatat aatacaattg tgtaagatat tgataaaaaa tcgtaataaa ttttcattag
      601 tatatgctaa tattttttta caattttcgt aattaattaa tagaaaaata tattataata
      661 atattatgaa aatttaaaaa agtaagaaag aggtaatatt atgagaagtg atataaaaaa
      721 aggaatagaa ggagctccaa agagagcgtt aatgtatgga atgggtctta ctaaagaaga
      781 aattgaaaga ccattaatag gtatagtaaa tgcacaaaat gaggtgattc ctggtcatct
      841 gcatcttgat gaaatagcag aagctgcaaa aaatggagta agaatgagtg gtggtttgcc
      901 attagaattt cctgctatag gggtatgtga tggtatagct atggggcatg ttggaatgaa
      961 ttattccctg gcttcaagag aattaatagc agattcaata gaagctatgg ctatggcaca
     1021 tggttttgat gctttggtac ttatacctaa ttgtgacaag atagtgccag gtatgttaat
     1081 ggctgctgca agacttaata taccaagtat tgttgtgagt ggagggccta tgcttccagg
     1141 taaaaaaaat ggaaaagtat atgattttaa ctctgcaatg gagggtgttg gagcatgtaa
     1201 agatggaaca gtaagtgaag aagaattaga aacgttagcc atgaactctt gtcctggatg
     1261 tggttcatgt tctggtcttt tcacagcaaa tagcatgaat tgtttaacag aagctttagg
     1321 catgggtata ccatataatg gtactgctgc gtcacattct ggagaaagaa aaaggatggc
     1381 aaaatatgca ggtatgtatg ttatggagtt acttaagaac gacataaaac ctagagatat
     1441 tttaacaata gatgctttta aaaatgctat agctgttgat atggcaatgg ctggttctac
     1501 aaatacagta cttcacttac ctgcaatagc ttatgaatca ggaatagagc ttaacttaga
     1561 tttttttgat gaaataagtg aaaaaactcc ttgtttaaca aaattaagtc caagtggaaa
     1621 acatcatatt gaagatttac atatggcagg aggaatacca gctataatga acgaactttc
     1681 aaagataaat ggaataaatt tagattgcaa aaccgtaaca ggcaagacta taggggaaaa
     1741 tataggaaat tgtgaaatag aaaatgaaga agtaatacat acattaaaga atccatatag
     1801 taaccaaggt gggcttgcaa tattgaaagg aaatcttgct ctaaatggag ctgttgtaaa
     1861 aaagtcagca gttgcagaag aaatgttagt tcatgaagga ccagcaagag tttttaattc
     1921 agaagaagaa gctgtaaatg ctatttttgg taaaaaaata aacaaaggtg atgttatagt
     1981 tataagatat gaaggtccaa agggtggtcc aggaatgaaa gaaatgctat ctcctacatc
     2041 agcagttgca ggaatgggac ttgataagca tgtagcactt cttactgatg gtcgtttttc
     2101 aggggcaact agaggagcat ccataggcca tatttctcca gaagctatgg aaggtggttt
     2161 aatcggaata gttgaagaag gagatataat ttctataaac ataccagaca aaaaattaga
     2221 gttaaaagta gatgaagttg aaatagaaaa tagaaaatta aaatttaaac ctttagaacc
     2281 gaaaataaag catggatact taagtagata tgctaaattg gtaacatcag caaatacagg
     2341 agcagtttta aaatagtaga ctatatttat gtaaattaga aaaagttaag aaaatattaa
     2401 ttggatatct taggtgttat agcactaatg aggtatcctt tttattttgt atgaaaaaca
     2461 cagcaaaatc ttgattatct ttgaaaacaa tacatctcta ttataaggaa tacgagtaca
     2521 gcatagagat gtatctttta gaccttgttg aggtaataca actttttaaa agtttgatgt
     2581 ggaatgcttt tcgttccatc tgaactatga agaaattgaa agtcagttaa ttcttgacat
     2641 aataaatatt aaaatgtata ataaaaaaca aacagacagt ctgtttttag gagggaaata
     2701 tgaatagaga agaaaaaagt aaaaatagta aagaaaaaat tatccaatca gcattttcac
     2761 tattttcgtc taaaggatat gattcaacat ctacacaaga tattatcaat ttatctggtt
     2821 tatctagagg tgcaatgtat catcacttta aaactaaaga agatatactg agaagtgtca
     2881 caaaagaact ttactcacaa atgaataatt ttttagagca tcttgttgct gatgacacct
     2941 ttacagcaaa tgaaaaaata atagaattgg ttgttcatag tgcgaatgat tacacacgta
     3001 gaaaaatggt acattgtagc tggttagaaa aaatcccatt cgctttaata gaggaagttc
     3061 gtaatctcaa caatgtagtt gcacccaata ttgctaagat aattaaacaa ggtgttgaaa
     3121 ataaagaatt ttcttgtgaa tatccagagg aattagctga aatgctcgtt tttagtattg
     3181 atattttact tgaccctgta ttatttaaac gtgaatacag tgaagtatgt aataggttgg
     3241 attttttatt attcatgtta aaaaaaatgg atattcctct aattgatgag tacggtattc
     3301 aaaaatttaa agatttattt aaacaatgat aataggaaaa gtgaggaata gactatgttt
     3361 tataatgcaa aaagctgtca aatgatgata ggaagtaata cagtgaatta tattgaattt
     3421 ggtagtggta agaagacact aattatttta ccaggattag gagatggttt atttccttta
     3481 catggaaaaa tacaggcgat tgcttttgct tttagatata agcaatttgc aaaagattat
     3541 aaggtttatg tatttagtag aaaaaatcaa attacagaaa aatattccac aagggatatg
     3601 gctaaagacc aagcagatat aatgaaaaaa cttggaatta tgaaagcaga agtaatgggt
     3661 gtttctcaag gtggaatgat agctcaatat ctggcaattg attatcctga gttagttgag
     3721 aaacttgtgt tggcagttac ttcttcaaag caaaatgata ctatccaaaa tgttatttgt
     3781 agctggattg atatggcgaa gaagcagaat tacaatgatt taatgattga cacagcaaag
     3841 aaatcctatt ctgaaagata tttaaaaaaa taccaattat ttattccttt tttaggaaaa
     3901 gtaggtaaac caaaggattt taaacgattt attattcaag cgacctcttg catagaacat
     3961 aatgcctttt ccgaattaag taaaataaca tgtccaacct taattatagg aggtgctaat
     4021 gataaaattg ttggaaataa tgcttctttt catttagcag aaaagattaa aaaaagcgaa
     4081 atatttatct atgagggatt gggacacgct acatatgaag aagcacagga ttttaatgaa
     4141 agagttttag agtttttaaa taagtaaaca cctttgttaa aaatatacat atgaagattg
     4201 gaaatttaat gttaaaaata gaaacatgaa aatatgctta actggtattt ttactattca
     4261 taaccaattt ttgatacatt atctactata aatacaaata tagcttcaat gtgattatat
     4321 attagtgtat tggtaaagca cttatacaaa cagaggaatt ttgtaaattc atattatacc
     4381 cacatttgtt aacttatgaa aatataatca aaatttttat gagattttta tataaaaaat
     4441 ataatgtttg tatacaaaaa tattgaagat attttagtaa gttttgtata taagcaaaca
     4501 tgtatttttt
//
LOCUS       DAFKMX010000242         4480 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFKMX010000242 DAFKMX010000000
VERSION     DAFKMX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.247"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     misc_binding    42..279
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            343..2262
                     /gene="thrS"
                     /locus_tag="LZB08_003460"
     CDS             343..2262
                     /gene="thrS"
                     /locus_tag="LZB08_003460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBM7316923.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3030..4121
                     /locus_tag="LZB08_003461"
     CDS             3030..4121
                     /locus_tag="LZB08_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBM7316924.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         1761 a          458 c          814 g         1447 t
ORIGIN      
        1 attgatgtat aactatctgt tataatgaaa atatattaaa aatcatagtg ttgaagagga
       61 agagtagagt agaaaccatt ctcagagaat ttgggtctat ggctgagagc ccatgtaata
      121 ggtctatttg aaaactacct tggagctgag cttattagca gtatttgttt tgtactatac
      181 gtgaaagtgc tcgggtgacg accttatagt catcaagagg gttgtatata cttgtatacg
      241 ccaacttggg tggaaccgcg aattaatatc gtcccaaaca tatttggggc gattttttat
      301 tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa caatgattaa agttgcttta
      361 aaagatggct caataaaaga atttgaaaat gctatttctg ttatggatgt agctaaatcc
      421 attagtgaag gattagctag aaatgtggta gcagcgtctg tgaacggaga ggttgtaggt
      481 ctggaccaca ttatagatac tgattgtgac ttgaatttat ttaagtttga agataaagaa
      541 ggtaaggaag tttttagaca tacatcagcg catatattag ctcaagctat aaaaagactt
      601 tacccagaag caaagcttgc tataggacca agtatagaaa atggattcta ctatgatata
      661 gatttagacc atagattagt tccagaggac ctagagaaaa tagaagctga aatgaaaaaa
      721 atagctaaag aagatttaaa aatagaaaga tttgaacttc ctagaaatga agctttagag
      781 cttatgaaag aacaaggaga agactataag gtagaactta tatctgattt acctgaaagt
      841 gaaataatat ctttctacaa gcaaggtgat tttactgatt tatgtagagg accacattta
      901 ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg tagcaggtgc atattggcgt
      961 ggtgacgaaa ataataagat gcttcaaaga atatatggaa cttcttttga gaaaaacaaa
     1021 gacttagaag agtacttaca tttactagaa gaagctaaaa agagagacca cagaaaatta
     1081 ggtaaagaat taggattatt tatgatacct gaagaaggtc caggattccc tatgttttta
     1141 ccaaaaggta tggaacttaa aaatgaatta ttaaaattct ggagagaaat acacagaaaa
     1201 gctggatata ttgagataga atctccaatc atattaaata gaaaattatg ggagacttca
     1261 ggacactggt atcattacaa agaaaatatg tatacagtta aaatagatga tgaggattat
     1321 gctataaaac ctatgaactg tcctggtgga ttaatttact ataactctca actacattca
     1381 tatagagatt tcccaatgag agttgcagag cttggtagag ttcatagaca tgagttatct
     1441 ggtgcattac aaggattaat gagagttaga gcatttactc aagacgattc acatatattc
     1501 atgttaccag agcaaattaa ggacgaaata aaaggagtag ctaacttaat agatggtata
     1561 tacaaaactt ttggatttga atataattta gaattatcta caagaccaga gaactctatg
     1621 ggaagcgacg aagagtggga agcagcagaa aatggattaa gagaagcttt agaagagtta
     1681 ggtctaccat atactattaa tgagggtgat ggagcattct atggtcctaa gatagacttc
     1741 catctaaaag attgtttagg aagaacttgg caatgtggta ctatccaatt agatatgcaa
     1801 cttccaagac aatttgataa tacttatatt ggtcaagatg gtgaaaagca tagaccagtt
     1861 atgattcata gggttgcttt tggtagtata gaaagattta taggtatatt aatagagcat
     1921 tatgctggta aattcccagt ttggttatct ccaactcaag ttaagatact tcctatatct
     1981 gataaattta tggattatgc aaatgaagtt aagaaagagt tatttgataa gggtataaga
     2041 gttgaattag atgatagagc tgaaaaaatt ggatttaaga taagagaagc tcagcttgaa
     2101 aaagtgccat acatgctaat agtaggtgaa aaagaagtag ctgataataa tgtttctgtt
     2161 agatcaagag ataaaggtga aataggtagt attaaattag atgaatttat agctagtatt
     2221 tcaaaagaaa ttgaatcaag agaaagtatt attcaagact aatatatatt atattagtag
     2281 ctaagatatt gttatataag ataagtttaa tcaatatgtt gatatgttga tgcatagtga
     2341 ttaaatttat gaaaagatga gtataattag aggtacagtt aatcatattt ttatgattaa
     2401 tgtacctcta gttttttgta tggtagattt attaataaat atatcataga agtgttttga
     2461 acatggaaat atttatgtgc attctttaaa taaaagttta ttttttgagt gataattaag
     2521 tgcttgttgg taaggttatg gctgtaaaaa tcataacttt attttcatat ttgttccatt
     2581 ttcataaaaa ttattatccc tttaagaaaa tttcttaatc ctcttgaaat atttcctttt
     2641 tagtctaaag ttaaatttct ttcttcaaaa tgaatattta aatgaactaa aatttaagta
     2701 cattttctaa tcttatgagt ctatttttca tagataattg ccctataata gaagaatcta
     2761 tgttggagct atgtggtgac aataacagca ttgtcagtga ttaattaatg ctttataaat
     2821 gattaattag tgttttatgt tatttttgga cttaccctct atacatagat taatttattt
     2881 aactttagat atagagagaa tgtcaagtac atacacagac attagagttt aagaaagtgt
     2941 ttatggagca agaactttta aaagaggtag tgacttagtt taatttcaag aaatgataaa
     3001 tacaaaagag gaaaaagaga ggtgttgaaa tgatagaaaa agtagaagaa aaatctaaga
     3061 ttattaaaac atcaaaagaa tataagcgaa ctataattga agaaaaagat aaagaatgga
     3121 ccaaaaaatt aaatgatata cttagtagaa gtagtttttc taatgatgaa ttagataaaa
     3181 tcaaggcgtt aattccaaaa gagattttga ctaatgaaaa tgttggagaa gttgtgactg
     3241 aagatggata tgctaagcct gagtatgatg aggtatttaa atcattgttc actttaaata
     3301 atgaatatga tttacttgta aattttataa atgatatcct aaaagatgct ccatatgcta
     3361 acaggaatat caaaccgttc actcaaataa agagaataat tagagttgaa actgacccaa
     3421 caattaatta cattggagag aaaagaccta gacttgatat tttggctgaa gatgaagaaa
     3481 ataatcatat aaatattgag atgcaaagag cttttgagaa cgattatttg gaaagagctg
     3541 aatattatct tagtcgtgta catggtagaa agttagaaga aggtaaagag tataaagaaa
     3601 ttggaaaaac tattggaata catatactaa atcatgtaaa atataatcac atagaagatt
     3661 atgtaaattg cctgagatta acaatggacg ggcatccaga tatttattct agtaaaactg
     3721 cattatattt tatagagctt ccaaaaattc ataaatctga ctatatagta aatagaatta
     3781 tgctatgggg aaaacttatt agtaatcctt ctaacttaga tgtaagagtt atagctaaga
     3841 atgattctat tattaaaaaa gcacttgata gattaaaaga attaggtagc aatgaagaat
     3901 atttattaaa tctgaaaaga ggtgcttata tcatgaataa aagtagaaat tttaaagaag
     3961 agattgaaag agagacagca attagagttg caaaagaaaa agattataaa ataattataa
     4021 tgttgaaaaa aaatggtttt aaattagatg acatattaaa taaatttaat gattttgact
     4081 taagtgaaga tgagataaaa gaagtatatg aaaataattg atgataaata aaaagtgaac
     4141 ttatataagt ttgctgaatc ggagtctgga aatatttatg tgtatttttt aatgaatgaa
     4201 aatttatttt aaaaacaata atttagaagt acagttaatt atatttttat gatactgtac
     4261 ttctagtttt ttctattgta gatttattat ttactaataa atctacaata gaaatgtttt
     4321 tgttgtaaga atcattttat ctaatgattg ttttttataa tagttaaatt tccatttgta
     4381 gtgtttaaat ataaatctat aacagcattt ctataaatgt tataaatcac tataatgatt
     4441 caactcaaag ttactgctta aagaagcaga gattttatca
//
LOCUS       DAFKMX010000243         4451 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFKMX010000243 DAFKMX010000000
VERSION     DAFKMX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.248"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(464..1621)
                     /locus_tag="LZB08_003462"
     CDS             complement(464..1621)
                     /locus_tag="LZB08_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBM7316925.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1775..2344
                     /locus_tag="LZB08_003463"
     CDS             1775..2344
                     /locus_tag="LZB08_003463"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBM7316926.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(2526..2942)
                     /locus_tag="LZB08_003464"
     CDS             complement(2526..2942)
                     /locus_tag="LZB08_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316927.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3168..4262)
                     /locus_tag="LZB08_003465"
     CDS             complement(3168..4262)
                     /locus_tag="LZB08_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316928.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1549 a          770 c          480 g         1652 t
ORIGIN      
        1 atttatatta taacctattt ttatatcgat aagtatgaaa ttgtttaacc aagtcaaaat
       61 gatatataaa tttatattta aatgtgttta gagtttaatt tattttcaat gtttaaaaaa
      121 gttattctat gcatctaatt taattagtaa actgaggttc tagttagata aattatatta
      181 taatgaatat ataatatgca gtttgcgata ttttaaaagt aaatgatata atatttggaa
      241 caactgggtt gcaatgtgtt tttaaaaaat atcaaaataa ggctatgatt tttaaaacca
      301 tagccctatc tgtaaaaatt taattatcac ttaaaaaata aatttttatt taaagaatac
      361 acataaatat ttccagactc cctcatgaac taaaaagttc attttgagac atctctctta
      421 taatgttctt tattttatag tataagttta taaaaattaa atcctattta tcaatctgtt
      481 ctctaagcca cttagttgct acatctaact gtttattttc atctttctct tcaatagtag
      541 gctttatacc tttttcattg atataatccc cattaggtgt aaaatattga gcagtagtta
      601 gcttgtatcc tgttccatcc ttcaatctta caactgattg taccaaccct ttaccaaaag
      661 ttgtagtccc tacagaaata ccttctttat tatcaacgat tgcagctgtt aatatttcag
      721 cagcagaagc actttctcca ttagttaaca ctactattgg aagtcccaat ttttctttat
      781 cagatttcag gtactcagta tttcccttat tatcttttgt atatactatg gttccttcac
      841 caattaactc atcagcaact tctttacata tatctagcat tccaccagga ttatttctaa
      901 ggtctattac taatcccttg atattttttg cttttaattc ttttaaagct ttatcaaagt
      961 cagtatatgt acttgatata aattgggtaa tttctatata tcctaaatca ttatcaatta
     1021 ctttactttc tatactcttc tcaattatct ggtctgtttt tactttaaag ttcaattgtt
     1081 tatcttctct taatatagtc agttcaactg ttttaccttt tttacccttc atcatacgaa
     1141 cagcttcatc tgaattttta tatgagacag attttccatt gacttttact aatttatccc
     1201 ctgattttac acctgctttc tctgctggag aatctttcat gggaactact actaagccac
     1261 catcttttga tgcccctata tacataccta caccaacata agaaccttca ctatcattaa
     1321 ttagattctc catttcatct ttagtataat aaccagaata cttatcattt gttcctagaa
     1381 atagaccttt tgctgctcca tttaccaaat cagtatctga tactttttta taaaaatctt
     1441 gttttataat agattctagt cctaacaact tatcatattt tttatagtct tcatagagtt
     1501 ctttcgatat tacaacttta ttccccaatg tcaactgaaa tgctgatgta accatagctg
     1561 ttataattac taaaatcacc cctaaaaaaa tagctttctt ctttgaaatc acaagataca
     1621 tctcctttta cataaactat tattattata tgactagctg ttatttttat tacatttacc
     1681 caaatatatt gctttattac aataaattat actaaaattt atattaaata caattgctgt
     1741 acacattata acatattaag ggaggaatta ttttatgaaa aaagaattta gaaaaaaagt
     1801 tattgaatca agaaaaaata agagtgcaaa cttcgtactt agtaattcaa atttaatcac
     1861 tgaaaaatta cttcaaatgg aagatataag aaatgcaact aatataatgc tttatctaaa
     1921 ttttaacaat gaagttcaaa cagataattt aatcaaaaaa cttttgtctc taaagaaaat
     1981 agtatcaagt cccatcacaa taaaagaaaa tcatacatta atccctactc aaataacaga
     2041 tttaaaagag ggattgagaa ttggagcata tggaataaga gaacctaacg aaaaatcacc
     2101 tacaattgat attaaggcct tagatgttgt aattgtacca gctgtagcat atgatataca
     2161 ttgctataga ttaggatatg gtggaggatt ttatgataga tttttagaaa acttaagaaa
     2221 agatgctata actatcggca tagcatttga cttccagata ttcgattcta ttccaaaaga
     2281 aactcatgat gcacaattag actacatagt tactgaaact agaattatca ctccaaatag
     2341 ttagaatatt aagtatttcc agaccccatt taatgtagta ataaatacta ctgttaatat
     2401 cttactttat aagcatttta agtcatggct tttacaacaa taagaaccat atacaattta
     2461 cttacattaa taaaaaccat gactttattg tacaatatgc agttatttaa ttataaaata
     2521 ccttcttatt tcgtcgcatt taattctatt ctatctggaa gttcactcat atttgtttca
     2581 acaactatgt atggaaaaga atcttcctta tctttatact ttatcttgac tctcaaaaca
     2641 tcctttcctt cctcaaccac actttctatt ttgtccattt ctataccata tttattattt
     2701 cctctagtca ctataatgta tattttgtta tcgattttag ccgctaatgc tctttcttca
     2761 tttgtatact tatccatcac atctaatatc ttttctggta ttgaatttct ttgtaatact
     2821 gtataatcta tgggtttaga tttctctaca aacatacttg gtatgaatat aaatccccct
     2881 ataacgaata caagagctat aactccataa attatacctt taatattaaa ctttacattc
     2941 aatcttatca ctcctcccat atattctaaa tatatgattt ataaacttat cttagactct
     3001 aaattattga ttagtatata tttatacata attataaata tatgtaatgt aactaaaaaa
     3061 catgtttttt ctatttcata ataaacgagc taaaaaataa aaagagctat atgaaaacaa
     3121 tgaaaatgtt ttcatatagc tcttgagttt acaatattaa aaaatactta tttgtcactg
     3181 attccttctt gatatccaga tgtttttgta atctgtctat aaagaacaca aaccagtatt
     3241 atgacgccca tatatccagt atatggatat aaaataccaa ccagcttatc aaatggtagg
     3301 aagccaccta taagacctaa tatagctgct attaaagtta ttaatttaaa cttaggatgt
     3361 ttatcatcta caaatctatt agttactgac cataataaag gtacagctgt tgtatatata
     3421 cctaatataa gaattattga gaataaaact ccaattactg gtgaaatctt atctgctaag
     3481 aataatgatg gtattgcttt tgaatataag tttcctatat cagaaagaat agctaagtgc
     3541 atcattatag ctgcagccat aagtgctact ccacctaata taccaccaca aatagcttct
     3601 tttttgttag cagcacttgc tcctaatcca gttaagaatg ccataacaac aattgtgtta
     3661 taagtagtat atattacacc agatagatag gcatttggaa ctggtttagc tatctttaca
     3721 gtgctaagaa cttgtgcagc ttctcctata ttacctatgt ttttagctaa acttatacca
     3781 ccaactaaaa gtgtaaagat tattattata ggccctattc cacctagtat ctttgataat
     3841 ttatcaagac ctagtgaaac agttaataat gctactactg ccatacctac tctacctaca
     3901 aatggattta atccatagta ttcagttaaa gttgctcctg ctcctgaaat cattactacg
     3961 aaaacaccaa ataagaataa aggtccaaac cattcaaaga atattcctaa atacttacca
     4021 caataaacac gataaatttt gataggttct ttaagttgaa gctctttacc ttttgacata
     4081 actgaagcac ccatccatga gaataatacc attgaaatta tcccaccaat aatacctgca
     4141 aaaccataaa aagagaagaa ttgcattatt tcctgtcctg ttgcaaatcc agaaccgatt
     4201 actgttgcta catacgcacc tgcgaaactg atgactgttt taacatttac tttttctgac
     4261 atatctcagt tacctcccca tgtgaaattt tttcttctga ttaaacaatt cgataaacta
     4321 cctacaattt cctttaacat aaaatacatg ataactgttt tatagctttt tagtataata
     4381 acttaaaact tataatctga ttttataccc tactcctcct ctcataattt ttttagattc
     4441 ttttatctaa g
//
LOCUS       DAFKMX010000244         4278 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFKMX010000244 DAFKMX010000000
VERSION     DAFKMX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.250"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            181..1875
                     /locus_tag="LZB08_003466"
     CDS             181..1875
                     /locus_tag="LZB08_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBM7316929.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2207..2317
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2421..2792
                     /locus_tag="LZB08_003467"
     CDS             2421..2792
                     /locus_tag="LZB08_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBM7316930.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2888..3835
                     /gene="trxB"
                     /locus_tag="LZB08_003468"
     CDS             2888..3835
                     /gene="trxB"
                     /locus_tag="LZB08_003468"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBM7316931.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3939..4256
                     /locus_tag="LZB08_003469"
     CDS             3939..4256
                     /locus_tag="LZB08_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBM7316932.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1773 a          393 c          789 g         1323 t
ORIGIN      
        1 atattagtta gttttgtgtt ataaaatatt gtaattccat taatcatatg ttattattca
       61 acatgattat aatataaaga atatttgata tatataataa tttataaaag agtattacat
      121 agtacatgta aatctatatt tataaataca ataaagtaaa gttgtaaagg agtgctttaa
      181 atgaagtttt cagagtttaa gtatgaaaga cccaattata atagtatgaa aaaagagttt
      241 ttgagttgtg tagaagatat aaataattct cgtaattata aagagcaaca aaaaaatata
      301 cataagatta atttattaag aaataaaata gaaacgttgt caaatatagc ttcgataaga
      361 tatagtacag acacatttaa taaattctac aaagaagaaa aaaattattg ggatgaatat
      421 atgcctttgt atgaagaatt aaattcatat ttttataatg ccattgtaaa ttcaaaattt
      481 aaatatgatt tgataaaaga gtttggagag caatttttca caatagtaga gtattcttta
      541 aaaagttttt ccaaagaaat aataagtgaa ttgcaggaag aaaataaatt gtgttcagag
      601 tatactagac ttttagcttc agctgagata atgtttgatg ggaaaataag aaatttatct
      661 ggtatgggga aattcatgta ttctaagaat agaaaaacaa gagaattagc aaacaaagca
      721 tattataatt tctttgaaga aaatgaaact aaatttgatg acatatttga taaattggtt
      781 aaacttagag ataagatgtc taaaaaatta ggttttgaag attttgtaga gcttgggtat
      841 gttagaatga tgagaagtga ctacagagaa tatatgataa aaaatgttag aaaacaagta
      901 ttaaaatatg ttgttccaat ggcaaatgag ctttatgaaa aacaagctaa aagaattggt
      961 ctagaatatt taagttacat agatgaaggc gtggaatttt taacaggaaa tgcaagttta
     1021 aagggagatt caagatacat aattcaaaat ggtaaaagga tgtattctga actgtcaaaa
     1081 gaaacaaatg aattttttga ttttatgtta gaaaatgaac ttatggattt agaaacgaag
     1141 aaaggaaaag gagctggagg gtattgtact tatataccag attataaatc accattcatt
     1201 ttttctaatt ttaatcaaac tgctgatgat atagatgtat taacacatga ggcaggacat
     1261 gcatttcagt tgtatatgtc tagatggatt gatatgccag aaattaattt tccaacgctt
     1321 gatagttgtg aaatccattc aatgagtatg gaatttataa catggccgtg gatggattta
     1381 ttttttaaag aagatacaga taagtacaaa ttcactcact tatcatcttc aattaaattt
     1441 ataccttatg gagtaattgt tgatgaattt caacactata tttacaaaaa tcctaatgta
     1501 gataagtcaa aacgaaaaga aatctggaga tttttagaga aaaaatactt acctcataga
     1561 aaatatggtg ataattcttt tttagagaga ggttgttggt ggtttaaaca aggacatata
     1621 tttaaaaatc cgttttatta tatagattat gtattagctc aaatttgtgc gttacaattt
     1681 tggaaaaaaa tgattcaaga tagagatgca ggatggaaag attatataaa tatctgtaaa
     1741 gttggaggaa caaaatcatt tttagatatt gtgagtatgg gaaatttgta ttctccattt
     1801 gatgatggct gtattgagtc tattataggt gatgttaaat cttggtttga tgaaataaat
     1861 gacagcaaat tataaaaaat tatgagaatt ttattgtata aaagtgaaaa attaaaaaat
     1921 atataaaaag tgattatttg ttgtgaaaat agtcttgttt ttaactaaat tgataaaata
     1981 aactaaaaaa gatttgtact taaaaaaaaa agaaaataca agaaatatgc aatatcaaca
     2041 tttggaaaaa aaagtaaaaa tgtatacaaa atacatataa aaaaactatt gcaaatttta
     2101 tagaatgttt atatacttaa gtcaacagag atagaaaaaa gctatttata gaaaacaaag
     2161 gataaattgc ttaatttaaa attttctgaa aaaaataata atccggatga agagtatggg
     2221 agactgcttt ataaaaagcg accgaaggga caagtttaat cttacttacc caaaagatta
     2281 gatgaaattc tcaggtaaag gtgactgtac ttggacgggg ctctgaaaat gattttaaca
     2341 gggaagacaa attgggagat ttgtttttcc tgttttttat ttatcaagta ataaataaaa
     2401 gtttgaaaga gagtaaaaaa atgataataa ttacgaataa tccaaaagta aaagaagaag
     2461 ttcaaggcag agaagttttg tttaaagaca caacttatat tggaatctta gaagcaagta
     2521 gagatttgat acatgaaggt tatgaacttt tatctcatcc actctatggg agtgtaaagc
     2581 caaatgaaac accttacagg actgttatac taaagaaagg aaatcgtttg gatataaact
     2641 ctttaacctt aattgaagaa gcaatcatca ctgcaagtaa atttcagaat aataaaaaga
     2701 ctccaaaatg gacagaaagt gttcaagatg attttagagt aattgattat gatatatttt
     2761 ataatacaat acaaagaatg cagtatgaat aaaaaaattt gttaaaaagt tattaaaaca
     2821 gcctaaatat taaatctaat ttaatattaa ataaaatgag aaatttaatt ggggaggata
     2881 agtcaaaatg gaaaatgtgt atgacttagt aataattggt tcaggaccag cagggcttgc
     2941 agcaggtctt tatggagcaa gagcaaagtt aaaaactcta atcctagaaa aagacaagac
     3001 tggtggacaa attgtaataa cacatgaaat tgcaaattat ccagggtcag ttccaaatgc
     3061 aacaggacca agtttaatag ctagaatggt tgaacagtgt aaagagtttg gagcagaaat
     3121 gctaagagac aacattgttg atactgagct agatggagat ataaaagttt taaaagggga
     3181 aaaggcagaa tatagagcaa aagcagtaat aataggaaca ggagcaacac caagaaagat
     3241 tggttgtcct ggagaaaaag aacttactgg taaaggggtt tcatattgtg caacttgtga
     3301 tgcagatttc tttgaagatt ttgaagtatt tgttgtaggt ggaggagaca gtgcattaga
     3361 agaagcaatg tatttaacta aatttgctag aaaagtaaca attgtacata gaagacaagg
     3421 atttagatgt gctaagagtg ttgaagaaaa agcaaaagct aatccaaaga tagagttttt
     3481 attagataca gtaattgaag aaatcaaagg tgatggaata ttagaatcag ttgtatttaa
     3541 aaataaagtt actggagaaa ctcatgaata ctttgcagat gaagaagatg gaacaatggg
     3601 agtatttgtg ttcgttggat tagatgctca aactgattta ttcaaaggta aagttgatat
     3661 ggatgaaaaa ggttacataa taactgatga agatatgaga actaacatac caggcgtatt
     3721 tgcagcagga gattgtagat ctaagactct aagacaggtt gtaactgcaa ctaatgatgg
     3781 agcaattgca tctatagtag ctgagaaata cattgatgaa aaatttggga actagaatct
     3841 gtaaattaag aaatagtgtc tgtaaattta atgaataata tttaaaatta aaattaatat
     3901 ttaaaattaa aataaaaatt tcaaggggga ataagaaaat gttagattta gataaggcta
     3961 catttgaaga agaagtttta aatgcagaag gttttgtatt tgttgatttt tggagtgaag
     4021 gttgtgagcc ttgtaaagct ttaatgccag atgttcataa attagctgag acttatgggg
     4081 ataaaataaa attctgtaag atggatacaa caaaagctag aagattagct ataaaacaaa
     4141 aagtattagg tcttccaact atggctatat acaaagatgg agaaaaagta gacgaagtta
     4201 ctaaggatga tgctacagtt cctaatatag aaaacatgat taaaaaatat ctttaatatg
     4261 ttgataaatt attaacat
//
LOCUS       DAFKMX010000245         4240 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFKMX010000245 DAFKMX010000000
VERSION     DAFKMX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.252"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..282
                     /locus_tag="LZB08_003470"
     CDS             <1..282
                     /locus_tag="LZB08_003470"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316933.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            540..1589
                     /gene="carA"
                     /locus_tag="LZB08_003471"
     CDS             540..1589
                     /gene="carA"
                     /locus_tag="LZB08_003471"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBM7316934.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1782..>4240
                     /gene="carB"
                     /locus_tag="LZB08_003472"
     CDS             1782..>4240
                     /gene="carB"
                     /locus_tag="LZB08_003472"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBM7316935.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTVEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMV"
BASE COUNT         1754 a          431 c          840 g         1215 t
ORIGIN      
        1 aatagcttaa gagaaaatgg aatagaggct gatatagtaa atagagtaga agaatctaga
       61 ccaaacatat tagatgctat aagaaataaa caagttgata tagtaataaa tacaccaact
      121 aaaggaaatg actctacaag agatggattt aagataagaa gaacagctat tgaattttct
      181 actgaaataa tgacatcttt ggatacacta aaagctttag tagaagttaa gaagaaacat
      241 ttaaataaag atgagttaaa agtttacaat atagctgaat agaaattaag ttgattgtat
      301 agtttaagaa tttaattatt aaataaaaaa aaagaggtta ttttttctag gagtttttac
      361 ttttgagata acttcttttt tttaagaaat ttgtagtata atagaaaaag ataaaaaata
      421 aatgtaaata agtataaaac tagaatgtaa aagtgaaaaa tagatataga tatttaaaag
      481 cttatattta gttaatgttc tttagtttgt ctaaagagaa ttataggagg tactgtagta
      541 tgaaagcaag gttaatatta gaagatggaa cagtttttat aggaaaggca tttggatatt
      601 tagaagaaag tgtaggagaa gtagtattta atacttcgat gataggatat ggtgaagttt
      661 taacagaccc ttcttactat ggtcaaatag ttactatgac ttatcctctt gtaggaaatt
      721 atggtataaa tttatcatca gcagagtctg aaaaagttca agtaaaggga ttcatagtaa
      781 gggagaaaag tgattctcca agtaatttta gatgtgaaat tgatatagac caatatttaa
      841 agcaaaataa agttatcgga cttgaaggaa tagatacaag agctttgact aagatactta
      901 gaaataatgg aacaatgaaa ggtataatta cattagaaga cagtaaatta gaagatgtta
      961 aacataaatt ggataagttt tcaaatacag aagcagttag aactgttaca agaaaagaag
     1021 tagagcatat aaaaggaaat ggtccaaaag tggctgttat ggattttgga gtaaaaagaa
     1081 atatattaag atcatttata gctcgtggat gtgatataac aatatttcca gctacaacat
     1141 ctccagaaga tgtattgagt ataaaccctg acttaatatt cttgtcaaat ggacctggag
     1201 acccagaaga cttagaagat gttatagaaa atataaaagc tcttattggt aaaaaaccta
     1261 tagttggaat atgcttagga catcaacttt tagctttagc tcttggagga aaaactgcta
     1321 aacttaaatt tgggcataga ggtggaaatc atccagttaa agatttggaa gaaggaaaag
     1381 tatttataac ttctcaaaat catggttatt atgtttcaga agttcctgaa caaatgaaag
     1441 tgacacatat aaatttaaat gataatactg ttgaaggaat gcgacatgag aagctagatg
     1501 tatacagtgt tcaataccat cctgaagctt gcccaggacc aaaagataat gactatattt
     1561 ttgataaatt tttagaatta gtaaaataaa taacattaga atagctaact aaaaaacaag
     1621 aataacttaa atcaaaaagt ttggtttaat taacatagat gtattaaata gatgtattga
     1681 tgagtaaatt tgaacttaag ataaaataaa gtaattaaag ttgattgtat agatgatgaa
     1741 gataagaact gtgaaaattg tttagataag gagaaagagt tatgcctaaa ttagatagta
     1801 taaagaaaac tttagtatta gggtcagggc caataataat aggtcaagct gcagagtttg
     1861 actattcagg aacacaggcc tgccaagcat taaaagaaga gggaattgaa gttgtattag
     1921 taaatagtaa cccagctact ataatgactg ataaagaaat tgcagataaa atatatatag
     1981 aaccattaac agtagaattt atagaaaaaa taatagaaaa agaaagacca gacagtttac
     2041 ttgcaggtat gggtggtcag acaggattaa acttagctgt agaattacat gatgctggta
     2101 tattagataa atataatgta aaagttatag gaacatctat agaatctata aaaaagggcg
     2161 aagatagaga tttatttaga gaagtaatga aagaaataaa tcaaccagta atagttagtg
     2221 atatagttac aaatttagaa gcaggtcttg agtttgccaa taagatagga tatccagtag
     2281 ttgtaagacc agcatatacg ctaggtggaa ctggtggagg tatagctgat actgaggaag
     2341 aattaagaga aatactttca catggactac agctaagtcc tgttggacaa gttcttcttg
     2401 aaaagagtat aaaaggttgg aaagaaatag aatatgaagt aatgagagat ggaaatggaa
     2461 actgtataac tgtatgtaat atggaaaatg tagacccagt tggagttcat acgggagaca
     2521 gtatagttgt tgctccaagt caaactctaa gtgatgaaga gtatcaacta cttagaaagg
     2581 catcaataga cataataaat gcaatagagg ttcaaggtgg atgtaacgtt caaatagctt
     2641 taaacccaca tagtttagaa tatgcgataa tagaaataaa cccaagagtt agtaggtcat
     2701 cagccctagc atcaaaagct acaggatacc caatagcaaa agtagcagct aagatagctt
     2761 taggttatac tttggatgag atagaaaatg cagtaactaa aaaaacttat gcttgttttg
     2821 agccaacatt agattatgtg gtagtaaaaa taccaaagtg gccatttgat aaatttagac
     2881 atgccaatag aaaattagga actaagatga tggcaactgg tgaaatcatg agtataggaa
     2941 gtaactttga ggctgctatt cttaaaggta taagatcact tgaaacagga aaatattcat
     3001 tggttcatgc tccatcagaa ggtagaacat tagaagaatt aaaggctaga gtagtagtac
     3061 ctgatgatga gagactattt gacttagctg aaatgataag aagaggctat aaagtagaaa
     3121 tgatatcaga aataactggt gttgataagt ggtttataaa taaatttaaa tggatagtag
     3181 aacaagaaga aaagttaaaa gttctaaaaa tagaagactt agataaagaa tatttacatg
     3241 aattaaagaa aaagggattt tctgataaag gtatatctga cttaatgaag ataagcccag
     3301 aaaaattata tgaattgaga agtttatata atatacaacc agtttataaa atggttgata
     3361 catgtggagg agaatttgaa gctctatcac cttattacta ttcaacttat gaacaatatg
     3421 atgaggtagt agttagtgat aagagaaaag ttgtagtatt gggttctggt ccaataagaa
     3481 taggtcaagg aatagaattt gactattgtt cagttcactg tgtaaaatct ttaagaaaaa
     3541 tgggaataga aactataatt gtaaacaata accctgaaac tgtaagtacg gattttgata
     3601 catctgataa gttgtatttt gaaccattaa cagaagaaga agttttaaat ataatagaaa
     3661 aagaaaaacc agaaggtgta atactacagt ttggtggaca aacagctata aaattggcta
     3721 agtttttaca tgaaaaaaat atacctatac tgggaacaga ctttagagat atagatgcag
     3781 cagaagatag agaaaaattt gatgatttac tagagagact agatataaat agaccaaaag
     3841 gaaaaggtgt atggacaact aatgaaggtg ttgaaattgc taaggagtta ggttatccag
     3901 tattagttag accatcttat gtacttggtg gtcaaggtat ggaaataact tataacgaag
     3961 aaaaactaat tcagtatttg gatgatgcat ttgatagaga ccataaaaat ccagtactta
     4021 tagataaata tctaactggt agagaaatag aagtagatgc tatatgtgat aaggaagata
     4081 tattgatacc tggtataatg gaacatttag aaagagctgg agttcactct ggagatagta
     4141 caacaatgta tccaagtcaa aatatatctg atgaaataaa agaaaagata gtagaataca
     4201 ctaaaaaaat agctttagac cttaatgtgc ttggtatggt
//
LOCUS       DAFKMX010000246         4200 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFKMX010000246 DAFKMX010000000
VERSION     DAFKMX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.253"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            446..1339
                     /locus_tag="LZB08_003473"
     CDS             446..1339
                     /locus_tag="LZB08_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBM7316936.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1339..2733
                     /gene="gltA"
                     /locus_tag="LZB08_003474"
     CDS             1339..2733
                     /gene="gltA"
                     /locus_tag="LZB08_003474"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBM7316937.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3099..>4200
                     /locus_tag="LZB08_003475"
     CDS             3099..>4200
                     /locus_tag="LZB08_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBM7316938.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGNKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNVKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKI"
BASE COUNT         1656 a          409 c          801 g         1334 t
ORIGIN      
        1 aagctggtat tgcaatagat taaagcctta tacacgttta gtttttaaag aaattagtgt
       61 tattatgaaa acctaagtta atttattaaa taaataattg aataaaaaaa ttttattatg
      121 tatttataaa atttttgtta caaatttggc ataatatagt atactttaag tgtgtattag
      181 gttaaatcta taacaaatta gacgtatgat atagacttag tgtttattgg catttacaag
      241 aaatttgtta atatcaacta tttgtagttt gggcagttat aattattata ggggggatgt
      301 ccaatacaaa acaatcaacc aagaactaat attatattat aaagagttat agttttgtgt
      361 gtttatgttt ttaattttgt gtgtttatgt ttttaattaa atttaaagtg ggaaaaatat
      421 atttgttaga atggagaata gcgagatgag taataaaata gttagtaaga gacaattaac
      481 agatagtatt tatttaatgg aaatagaggc acctagagtt gcaaaatctt ctcaaccagg
      541 tcaatttata ataattaaaa atgatgaaaa gggagagagg attcctctta caatagctga
      601 ctatgataga gaaaaaggaa cagtaactat tgtattccaa acagttggtg cttcaactaa
      661 aaagttagca atgtttgaag aaaacgattt tgtaatggat tttgtagggc cattaggaca
      721 agcaagtgaa tttatacatg aagatataga agaattaaga aataaaaaga tattatttgt
      781 agctggtgga gttggttctg ctccagttta tccacaagtt aaatggttta aagagcatgg
      841 attagatgta gatgtaataa ttggtgcaag aactaaagaa ttaattatat tagaagatga
      901 tatgaaaaaa gtagctaaaa atgtatatgt atcaacagat gatggtacat atggatttaa
      961 tggaagagtt acagacctat taaaggattt agttgataat caaggtaaaa aatacgacca
     1021 agcaatagta attggaccaa tgataatgat gaaatttatg tgccaattaa ctaaagaact
     1081 aaatatacct actatagtaa gtttaaatac aataatgata gatggaacag gtatgtgtgg
     1141 tggttgtaga gttagtgttg gaaatgaaac taagtttgca tgtgtagatg gtcctgaatt
     1201 tgatggtcat ttagttgatt ttgatcaagc aatgagaaga cagtctatgt acaagactca
     1261 agagggaaga gctatgctaa aacttgaaga aggcgatagt catcatcata gtaattgtgg
     1321 atgtggaggt aacaaataat ggatgctaaa aaagtaaaag taccagttag agaacaagaa
     1381 cctgcagtga gagcaacaaa ttttgatgag gtatgcttag ggtataataa agaagaagct
     1441 atggcagaag ctaatagatg tttagcttgt aaaaaaccaa aatgtgtggg tggatgtcca
     1501 gtaggaatcg acataccagg gtttataaca aaaattaaag aagatgatat agaaggtgct
     1561 gctaaagtaa tagctaagag cagttcatta ccagctgttt gtggtagagt atgcccacaa
     1621 gaaagtcaat gtgaaggtgt atgtatactt ggaataaaat cagatgctgt atcaataggt
     1681 aaattggaaa gatttgtagc agattggtca aaagaaaatg acataaattt atctgataca
     1741 gagcctaaga aaaatcaaaa agtagcagta ataggaagtg gtccagcagg tctagcttgt
     1801 gctggtgatt tagctaaaaa aggctatgat gtaacaatct ttgaagcaat gcatgagcca
     1861 ggtggagttt taacttacgg aatacctgag tttagacttc caaaacaagc tgttgttcaa
     1921 cctgaaatag acaatataag aaaattaggt gtaaaaatag aaactaatgt aattgttggt
     1981 aaaacaatta cagtagatga acttatagaa gatgaaggat ttgaagctat attcataggt
     2041 tcaggagctg gtcttccaat gtttatgaat ataccagggg aaaatgcaaa tggagtattt
     2101 tctgcaaatg agtttttaac tagagtaaac ttaatgaagg cttatagaga tgattatgat
     2161 acacctatta gttctggtaa aaaagttgct gtagtaggtg gtggaaatgt tgctatggac
     2221 gctgctagaa ctgcattaag attaggttca gaatcatata tagtttatag aagaagtgaa
     2281 aaagagcttc cagcaagagc tgaagaagtt catcatgcaa aagaagaagg cattatattt
     2341 aatacattaa caaatcctaa ggaaatttta gtagatgaaa atggatatgt taaaggtatg
     2401 gtttgtataa gaatggaatt aggagagcca gatgattctg gaagaagaag acctatagaa
     2461 atagaaggtt ctgaatttgt acttgatgta gacacagtta taatgtcact tggaacaagc
     2521 ccaaatccac taatatcttc tacaacaaag agtcttgata taaataagaa gagatgctta
     2581 ataacagatg aaaatggtca gacttctaaa gagggtgtat ttgcaggtgg agatgctgtt
     2641 actggagcag caacggtaat atctgcaatg ggtgctggta aaacagcagc agcatcaata
     2701 gatgaatatt taaaagcaaa agttaatgct taatttgtaa attaaagaga gggtgtctca
     2761 aaatgaactt tttagttcat gaggcactct ttttgtacaa aatcaaatat gttttaatca
     2821 tttcaacaat gaaaatttca ttcctgtaga aagtctaact tttaggtctt agtacaatct
     2881 ctattttgat gtagccactt ttttagatag tttaataatt tatcagttat tattgtaata
     2941 tttgatacta agtaacataa ctttgtctaa ctatgataaa atagttatac aataaaggtc
     3001 ttgtaaggag tgaataatgt gtggctaata aaatcagatt tgtcaaatct aacattaaat
     3061 aatagaaaaa taaattgtgt aattaatggt attttaatat gaaaaaaaga gaaaaagtat
     3121 ttgatgggct agtttttaag catatattaa tcttattaat actattagtt ttgattgatt
     3181 taacaagtac catcatatat gcaagtaact ctatggaaat tacttctaga aatatgattg
     3241 aggcatcaaa aagagaactt gaaaactatt tagaagtaaa catatcttta ttaaaagctc
     3301 ttagtcaaga tgatagattt tctaatggtg aaacatcact aattgaaaag ggaaaattat
     3361 taaggccata tcaaaaggaa tataatcttt ttatgattgg tataacagat acaaaaggaa
     3421 atgcttcaag tacatatagg gaaaatgtta actcaataaa agataagcct agttttgaga
     3481 aagcaattaa aacaaagcaa gtagtagttt cagatattga agtcagcaat gtaacaggta
     3541 acaaagtatt tatcatatat gtaccaattg taaaaaataa tgagatgatt gggaccattt
     3601 ttgcttcttt ttattttcaa gatgtaaata atttaataag tagaagcaat tttgatgatt
     3661 ctattaaatt tctaatgata gataaagatt atactattat ttctcatcca aacaaaaaat
     3721 atgtaaatga taaaagtaag atgcttgatt tagaagggaa tattatagga accactaaaa
     3781 gtgaaatctt aaaaaatata aataaaaaat gtcaaggtga ttttttatct tgggataatt
     3841 ggagattata taatgtaaaa tacacgaacg taaagtggac taattggact ttagtatcaa
     3901 aatgtaatat atttaagaac tttaaaaatc taatagttaa ttttatgata aagttgtatt
     3961 tttatatagt aatatttatg atattgtgga aattaagtaa tgctaaattg atagaacaac
     4021 ttaaaaaatt ggcttattat gattctttaa gtggaataaa aaataaagaa aagtttagaa
     4081 aagattccat gtatatttta aagaattatt atcaagataa tttttactta gtacaattgg
     4141 atgtcaataa atttaaatat ataaatgaga tgtttggata tgctgaaggg aataagatat
//
LOCUS       DAFKMX010000247         4142 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFKMX010000247 DAFKMX010000000
VERSION     DAFKMX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.254"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            16..414
                     /locus_tag="LZB08_003476"
     CDS             16..414
                     /locus_tag="LZB08_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7316939.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            402..1340
                     /locus_tag="LZB08_003477"
     CDS             402..1340
                     /locus_tag="LZB08_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBM7316940.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSILTVFLIIFILKQNNLI"
     gene            complement(1724..1948)
                     /gene="secG"
                     /locus_tag="LZB08_003478"
     CDS             complement(1724..1948)
                     /gene="secG"
                     /locus_tag="LZB08_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBM7316941.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2553..3845)
                     /gene="eno"
                     /locus_tag="LZB08_003479"
     CDS             complement(2553..3845)
                     /gene="eno"
                     /locus_tag="LZB08_003479"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBM7316942.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1436 a          663 c          462 g         1581 t
ORIGIN      
        1 tcttaaggag ttgccatgat aaaaaaaata gaacatgtgg tcattactaa taataatata
       61 gaaaattata tcaattttta taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat
      121 tataaactta atgctagaaa tattaagata aatcttaaca taaaaggtta taaattttta
      181 ccttatgtta aaaattcaaa agcaagtaga gataacttat gttttaaaat aaacacaaat
      241 attgatgagc caaaaaaata cttagaacaa aatgagattc aaattgagcg aggaattgta
      301 gaatgtacag gtgtctttgg taatatgaaa tctatttatt tgaaaaaaac taatggaaat
      361 ttaattgaat tatcaaatca tagaatcacg gagagtaaac cttggaaaat ttaatcctat
      421 caataaatgt tgttcttcct ttgtttctca caatgtcact tggatatgtt ctaaaaaaat
      481 taggtctatt tgatactcat actttaaata aaatgaataa tgtctgcttc aagtctttct
      541 tgccattatt attatttttt aatatataca atacagattt agatggttct cttaatttta
      601 agcttatggc tgtagctgtt ataattattt tttcaatctt cttaatctta gtctttatta
      661 ttcctaaaat tgaaaaagat aataagacaa ggggtgcatt aatacaaggt gtatttcgta
      721 gcaatttcgt tatatttggt attcctgtga caatttcact atttggcgat aagagtattg
      781 gagtcacatc tttattaatt gcagttattg tacctatgtt taatgtatta tctgttgtaa
      841 ttcttgaaat ttttagaggt ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa
      901 caaatccact cataatcgcc tcttttatag gattgacaat gctaatttta aatattaata
      961 tacctacatt tttacacaaa actattggtg atatttctaa aatagctact cctttatcac
     1021 ttataattct aggcggctct tttagttttt ctgctataaa ggggcatata aaacccatta
     1081 tattgggcgt atgtggaaaa cttatacttg tacctgctat atttttacca attagcattt
     1141 ttcttggatt tagaaatgta gaattagctt cacttatgat aatgttatct gctccaactg
     1201 cagtttcttc atttacaatg gcacaacaga tggattctga cagtgaatta gctggacaaa
     1261 ttgtagtatt tacttctgga ttttctatac ttacagtatt tttaataata tttattttaa
     1321 agcagaataa tcttatttaa tgatatttga taacacaaat tggtattatg agaaacatat
     1381 ataaaattga tttaactgcg taaaaacata ttattatact atttttacta tataatataa
     1441 ctttgtgtat attatctcat taaacatggt gaaagtagtg ttgctatata ttttttagtt
     1501 tgaagaaaat atataaatac aaacaatata taaatacaaa taataaacgc caacaaatag
     1561 tcaatctata aaataaaaac tggactaatc atataaaatc attagtccag cttttatttt
     1621 acgtactttt tatgttgatg tacttttatt aaaaactaaa tttaattttt aaatgtctag
     1681 caaatgcact aattaaataa tggccagtta ttcaacaata tcactacttc ttaactataa
     1741 gtaatgccaa tgcatttata aaaaataata ctgctgttat ctttgtaact ctagcaagaa
     1801 atgcttgttt tccttttggt ttaaagtaag cttgacttcc ttctccacca aattctgaag
     1861 gtaaagcatt cttagaatct tgaggcatta tgctaattac tagtaaaatc cctagtacta
     1921 cttgcacacc cattaaaatg ttgctcattc ttgcccctcc tatctgttta ttacttaaat
     1981 ttcattactc tttagtgtat cacaataata tagccatttc aagactatta tgctataaat
     2041 aacatacaaa aaaatatatt gttactataa tatattacta tattctcaat ataaaaatga
     2101 ataaaactta gaagagaaaa ctatttaaat taatacataa attaatgcat aaattaacac
     2161 ttaaaataat ctactctcat tgcatatatt ataaatagcc accaaaataa aacaatcatt
     2221 ttaaaatatt gatataactt ttaatttcaa cataatttaa aataaataat ccacatttag
     2281 caataatact ttaatgacaa ttatgattaa atcaaaatat gtcttcactt ttatttttta
     2341 ataaaaacct cctatgcaat tagataaaaa tgagatagaa acatttattc atattttttc
     2401 tgaatctatg aataaaatta agtaataaaa tataaaaaag gataagtgaa ttgaaaaatc
     2461 aattaacact tatctctttt atatcttata agatgagctt aaatatctta tattctctct
     2521 tcattaatct tttttagtaa ccattttaat aactattttt ttaagttata gaaagatttt
     2581 aatccacaat atctagcttg ctctccaacc atttcttcta ttcttaataa ttggttgtac
     2641 ttagcaactc tatcagttct tgatggagca ccagttttta tttgaccagc atttactgca
     2701 acagctaaat ctgctatagt agaatcttca gtttctcctg atctgtgaga tataacagca
     2761 gtatatcctg ctctcttagc catttctata gcatctaaag tttcagttat tgtacctatt
     2821 tgattaactt taactaatat agagttagct actccatttt caattccttt ttctaatctc
     2881 tctgtgttag taacaaataa gtcgtcacca actaattgta atttatttcc taatttttca
     2941 gttaataatt tccatccatc ccaatcttct tcgtctaaac catcttctat agttattatt
     3001 gggaagttgt ttgaccaatc ttcatataaa gcaaccattt cagcagcagt taactctttt
     3061 ccttctcctg ctaaaacgta ttttttagtt tctttgttgt acatttctgt agcagcaacg
     3121 tcaagaccta acataacgtc ttcacctggc ttatatccag cttttgttat agcttcaact
     3181 attaattcta aagcttctct gtttgaacct aagtttggag caaatccacc ttcgtcacct
     3241 acaccacaag ctaatccttt ttctcctaaa actttcttta atgaatggaa tacttctgct
     3301 cccattctta aaccttcttt gaaacaacaa gctcctactg gtaatatcat aaattcttga
     3361 acatcaacgt tgttatcagc atgctctcca ccgtttaata tgttcatcat tggaacaggt
     3421 aattgtttag catttactcc acctaagtat tggaataatg gtaaacctat ttcatcagca
     3481 gctgctctag ctactgccat tgaaacacct aatatagcat tagctcctaa tttacctttg
     3541 tttggagttc catctaattc tatcatgatt gcatctatag ctggttggtc aaatgcatcc
     3601 attccttcta tttcaggtgc tattatttca tttacgttag caacagctgt ttctacacct
     3661 tttcctaagt atcttccctt atctccatct cttaattcaa cagcttcaaa agctcctgta
     3721 gatgctcctg atggaactat tgctcttccc attgctccat cttctaatac tacttcaact
     3781 tctacagttg gatttcctct tgagtctaat acttctctag catatactaa ttcaataact
     3841 gacatttgta tatctccctt cgattttcta ttagttaaat tttcatttac tgtaaacatc
     3901 cgtctactat tttaatctac tttctatatt atagtacata aagttgcttt tgaaaattag
     3961 gtacaaattt atttgatggg taaatttgta aaagttccta tttttatttt attttttata
     4021 atattttaat ttatataata taaaaaaact tgtctaaaat ccaaaaccaa tcttaaatac
     4081 acaaatatta taactagatt taaaacatta aacataaaat gtacaactaa tgattctaaa
     4141 at
//
LOCUS       DAFKMX010000248         4125 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFKMX010000248 DAFKMX010000000
VERSION     DAFKMX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.255"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            160..2259
                     /gene="nrdE"
                     /locus_tag="LZB08_003480"
     CDS             160..2259
                     /gene="nrdE"
                     /locus_tag="LZB08_003480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBM7316943.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2272..3243
                     /gene="nrdF"
                     /locus_tag="LZB08_003481"
     CDS             2272..3243
                     /gene="nrdF"
                     /locus_tag="LZB08_003481"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBM7316944.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLENEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3319..3945
                     /locus_tag="LZB08_003482"
     CDS             3319..3945
                     /locus_tag="LZB08_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBM7316945.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDTRKVSPESLKTVLRFEVEREV"
BASE COUNT         1647 a          447 c          767 g         1264 t
ORIGIN      
        1 agcactgact atatgtagtg cttttgtttt tgaaaaatac ttattgcatt tctataatta
       61 tagattaatt ttgggatttc aactgataag taccaaaaga aataataagt aaattaagtg
      121 aaattatggc acaagattat gttaaaagga cggtaaataa tgaattggat agagcttaac
      181 aatcaagtga taattaaaaa tgaacaaggc aagtaccaat tggaaaagga taaggaagcc
      241 ttaagtagtt acatagaaga atttatacag ccaaagttga gaaaatttaa taacctagaa
      301 gaaagattga agtatttaat agatgaaggt tattattcaa aagaagtaat aaatcagtat
      361 agcatagatg ttattaaaaa tatatatgat atgattgata aacataagtt tgaatttcaa
      421 tcatatatga gtgcaaataa gttttatcaa aattatgcat taaagagcaa tgatggaaag
      481 gaaatattag aaacatataa tgataaagtg ttaattgtag ctttgacact aggaaatggc
      541 aataaagatt tagctttaaa cttggcagat aaaatagtaa aacaagaatt tcaaccagct
      601 acacctactt ttttaaatgc tggaagaaaa agggctggag aaatggtatc atgtttttta
      661 ctatctgtag aagatagtac agaaggtata tcgtatgcta tatcttcatc aaatcactta
      721 tcaaagattg gtggaggagt tgcactaaat ttatctaaac ttagagcatc tggcgaatct
      781 atcaaagata tagaaggtgc agctggaggt gttgttggag tagctaaaat gttagaacaa
      841 tcatttagtt actttaatca aatgggagct agacaaggtt ctggtgcggt gtaccttact
      901 gtattccatc ctgattttga attgttgatg gatactaaaa agataaatgc agatgaaaaa
      961 ataaggcttg ctacattatc attaggagct ataataccag ataaatttat ggagttggca
     1021 gaaaaaaatg aagtagcata tgcattttat cctcatactg tgtataagaa atatggagtt
     1081 tctcttgatg agatagaaat ggataagtgg tatgataaat tggttaatga tagtgatata
     1141 agaaaaaaag aaataaatcc taggcagatg ttgacaaaaa tagcacagat gcaacaagaa
     1201 agtggatatc catatgtagt gtacatagat acagctaata gagaacatac attaaaagat
     1261 gttggaatta ttaaaatgtc taacttatgt tgtgagatat ttcaatatca aacaccttca
     1321 gaaatagagg ggtatggagg aaaaaatgaa tggggacaag atataagttg taacctaggt
     1381 tctttaaaca tagccaatgt aatggataat aaaactatag aaagtacagt tgaaacagca
     1441 ataagagcac tgtcatttgt agcagatagc acagatataa aaccagtgcc aacggtaagc
     1501 aatagtaaca gcaagtctca ttctatagga ttaggagcta tgaatttaca tggatactta
     1561 gtaagagaaa atattttata tacttcagat gatgctatag atttttctaa tgtatttttt
     1621 gcaatggtta gatactattc aattaaagct tctatgaaaa tagctataga aagaaatcaa
     1681 acttttgaag gatttgataa atcagaatat gttaagggaa gaaatagcaa ggttttatca
     1741 aaatactatg aacaatctta tctacctaag tcagaaaagg taagagcatt atttgaaggg
     1801 atttatatac caacaaaaga agactggaca aaattattgg atgaggtaaa agaaaaaggg
     1861 atatataatg catatttaat ggcagtagca ccaactcaaa gcattagtta tgttcaaaat
     1921 gctacatcaa gtataatgcc aattactgag ccagtagaag taagaacata tggagactct
     1981 actacaatat atccaatgcc atttttaaca aatgataata tgttgtatta tcaatcagca
     2041 tacaggatgg atatgagaaa ggttatagat ttagtggcaa cagttcaaaa ccatgttgac
     2101 caaggtatat caacaacact gtttgttaca gatgaaaaga caactagaga tatagctaga
     2161 cattacatat atgcctataa aaaaggtttg aaaagtttat attacacaag aacaaagatg
     2221 acaagagata ctcatgagtg tctagtgtgt tcagtatagg gagagaaata tatgacattt
     2281 aatttacata aaatacataa tgcagttaac tggaatagag aagaagatgg atttacacaa
     2341 gctttttggg aacaaaatgt aaaacaattt tggcttcctg aagaaatatc tgtttctaaa
     2401 gacataaagg tatggaatga actaagtaat aaagaaaagg aattatataa aaaagtattg
     2461 ggtggtttaa cattactgga tacaaaacag gggaataatg gaataccatc tatgatgagt
     2521 ttaacagaaa acctacaaag aaaagctgtc ttgtctttta tggggactat ggaagaaata
     2581 catgcaaaaa gttattcttc tatatttatg actctacttt ctaatttaga aatagatgaa
     2641 ttatttgaat ggatagaaac agaaccaaca ttacaaagaa aggctgattt agttttagct
     2701 caatacgaaa atactacaaa tcaagaggga ttatacctat caatggttac aagtgtattt
     2761 ttagagagtt tcttgtttta ttcaggattc ttttatccat tgtatctatc tggtcaagga
     2821 aagatggtag ctagtggaga aataatatcg cttattctta gagatgagtc tttacatggt
     2881 aagtatatag gattgctagc acaagaaata tacgattcat ttgataaaat ggataaggag
     2941 atgttagaag agaagatgta ttctatactt tatagcctta tggagaatga gatagagtat
     3001 acaaatgtta tatacagaga aagtgggctt gaaaatgagg ttgtgaactt tttaaaatac
     3061 aatgcgaata gagctttaga aaatctaggg tttgagaagc tatatactgt agatgctata
     3121 aatccaattg tattaaatgg tcttagcact gaaaccaaaa cacatgattt tttttcaact
     3181 aagggtaatg gataccaaaa aggagtgtat gaggaattag aggatgaaga ttttataata
     3241 taatttataa ttatgacaca ctgttgtcac ttttagcgtg ataatatcaa tgtataaaat
     3301 atatatggag gtatttttat gaaatatgaa tggagaaaaa gagataaaga gttatattta
     3361 cctaaaaaga atcctgtaat agttgaaata ccagaacaga aatttatcat gttaaaaggg
     3421 agtggagacc caaattctaa agaatttact gaagcaatag gagtacttta ttctttggca
     3481 tatgcaataa aaacaatgcc taaaaaagga attgtaccag aagggtattt tgactatacg
     3541 gtttttcctt tagaaggtat atgggataag ggagaaaaaa gtaaaggctt agatgtactt
     3601 attaaagaag atttaattta cactataatg atacgtcagc cagattttgt tacacaagac
     3661 ctgttggata aagctactga tatagtaaag aagaaaaaac ctcataatct tcttgaaaaa
     3721 gttagatttg ggtctattaa tgaagggcta tgtgttcaaa tgcttcatat aggttcatat
     3781 gataatgaac cagaaagctt tgacattatg aacgaatttt gcaaaaaaaa taaacttata
     3841 agaaaatctt acacacatcg tgaaatttat ataagtgata ctagaaaagt atcgcctgaa
     3901 tcattgaaga cggttttacg ttttgaagta gaaagggaag tataaatatt ttgaataatt
     3961 tataagagtt ataaaaagtg aagtctgttg agacttcatt ttttgatgca attttaaaat
     4021 taaatttgat gtttcataat attttaaaaa ctttctactt ttattagtgt gaataactgt
     4081 tgctattaaa taataatact aattatataa gtattattat ataat
//
LOCUS       DAFKMX010000249         4115 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFKMX010000249 DAFKMX010000000
VERSION     DAFKMX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.256"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1143
                     /locus_tag="LZB08_003483"
     CDS             <1..1143
                     /locus_tag="LZB08_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBM7316946.1"
                     /translation="NIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            1512..2732
                     /locus_tag="LZB08_003484"
     CDS             1512..2732
                     /locus_tag="LZB08_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBM7316947.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHISDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEKKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            2769..>4115
                     /locus_tag="LZB08_003485"
     CDS             2769..>4115
                     /locus_tag="LZB08_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7316948.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         1648 a          496 c          649 g         1322 t
ORIGIN      
        1 aacatataca atagagatga aaaaatgggc acatattata gtttacaaga aataacatac
       61 ataaaaaaat tagaacaaaa tctcactaaa aaattaagag aaaaaggaca aatagcaaaa
      121 tatacattta aggatataaa aacaaatagt cctaaaatgt ttgaatgtat agatttagct
      181 aagaaagttt ctaaatctga tttaagcata cttatcagag gtgaaagtgg aactggtaaa
      241 gaactcatag ctcagtctat acataacaac tcaaatagaa aaaatcaacc ctttatagca
      301 gtaaattgtg cagccgttcc tgaaaattta ttagaaagcc agctattcgg atatgacaag
      361 ggaactttta ctggaggtct aaaagatgga aaacaaggat tatttgaact tgctaataat
      421 ggaacaatat tcttagatga aataggcgat atgcctttag aattacaaac aaaactactt
      481 agagttcttc aagagaagca gataatgcct gttgggtctc acaatgtaat taatatagat
      541 gttagaatca tatctgctac aaataagaat ttagaacaaa tgattgataa tagccaattt
      601 agagaggatt tgtattatcg attaaataca attccaataa atatacctcc tctaagagaa
      661 agaaaagaag acatattaat tattatggaa gatttaatta ataagaaatt agtaatcaca
      721 ccagaagcaa aaaaacttat acaaaactat atgtggaaag gtaatataag agaactccaa
      781 aatgttacat cctatttaaa cattatgtgt gaagatattg tattagagaa agatttacct
      841 ccaaacttaa ggtcttcaga taacaaaaat acatcattaa aactaaaata tagtaagaat
      901 gatatattga atatattgga gatactaatt ttaaataagg aatcagatgt tggtattggt
      961 aggggattaa ttttaaaagc actactcgat aagaaccttc aaattacaga aggaaaaatt
     1021 aaaaaaatat ttgaatatct aaaaaaagaa gaacttataa tttgtagttc aggaagatat
     1081 ggaagtaaaa taactcaaaa aggagaagat ttttacaata aattaaaata taaaggtcta
     1141 taaaattata atttatcaaa caaagaaatc tattcaggat taaagtactc cttcaatagg
     1201 tttttttatt tttcaagtac cctaaaatta taatttattt tgtacataaa taataggaat
     1261 taataggaat aggataattt ttttatttcc tatttttttt atataaacta gcatataaaa
     1321 tatattttct tcaaaataat attttttact gaaaattgaa actatgcaat cattttttat
     1381 actatacata tagataaatg attatattta ttcaatattc ttataaaaaa caaaattttc
     1441 catcgaaaat tatggcataa taattgctta ttatattaat aaaacaataa aaagcaattg
     1501 gaggaataaa aatgcaacag gctttgaaat taaaatatca aaccaaagaa ttaggaaaag
     1561 attttgtaat agaaagttgt aattctataa aaccatggct tataaacata agaagagaat
     1621 tacataaaat tccagaatta gctcttgaag aaaatcttac taaacagaag gtaatttctt
     1681 atttaaaaga aatcggaatt gattatatgg aatttacaaa acacaatgga attatggcat
     1741 atattttaaa agaaagtgca gataaaacga tttgtataag agcagatatg gatgctcttc
     1801 ctattgaaga agaaaacaac ataccttata aatcaataca ttctggaaaa atgcatgctt
     1861 gtggccatga tgcacataca accatgctac ttggagcatg taaagtttta cattcaataa
     1921 aagataaact taatgtaaat gtaaaattct tatttcaacc agcagaagaa ggttttggag
     1981 gagcaaaatt tttagttgag gatgggtgtt tagaaaaccc caaggccgat tatatattcg
     2041 gacttcatgt tatgccacat atagaaacag gccttatcga aacaaaatat gatacattaa
     2101 atgctagtgt agacactata aaaataagta tcaaaggtaa aagagctcat ggtgcatatc
     2161 ctgaaaacgg tattgatgca atagttacag cttcacaaat tgttacatca ttacaaacta
     2221 taataagtag aaacttagaa ccaaataatg ctgctgtttt gactataggt aaaatatatg
     2281 gtggtgatgc acacaatgta atatgtgaag atgtaaaatt agaaggcact ttaagaacct
     2341 taaatagcaa aacaagaaac tttatgattg ataaaatagc taaaattgtt ggagatactg
     2401 catctgcatt tggttgtgtt gggactttac atataagtga tgaaaactat cctgctgtta
     2461 taaatgaaaa agaattagta gatacagtta tatcaagtac taaagaatta cttggagagg
     2521 aaaaatttat attgagacca aacccgtctt taggtggtga agacttttct ttttatacag
     2581 aacattgtaa aggagcattc ttccatttag gatgtaaaaa tgagaaaaag ggcttaattt
     2641 cacctcttca tacctctagc tttaatatag atgaagattg tttaccaata ggtgttatga
     2701 tgcatgtaat gaatactttg tattttaatt agtacagtaa ttaataaaag aataggagat
     2761 aaattaatat ggaaaaagct aagaaattag gtctatttag tatgatatta ttagggatta
     2821 atagcataat aggttcaggt atattcttac taccaggtaa agtttataat ctagcaggtc
     2881 aaaacagtat gtttatatac atttttgcta cactcttagt tctatcaata cttttatgtt
     2941 ttgcagaagt tggaagcatg tttgataaga atggaggagc atatctatac tccaaaaaag
     3001 cttttggaga ttttatagga tttgaagttg gtacaatgtc ttgggttatt agaattatat
     3061 catggtctac tctagcagta ggatttgcaa cagctttagg ttcattttgg ccagaatctg
     3121 ccactgaata caaaggttat atagctgcaa tactcgtaac attactttca ataaatagct
     3181 tatttggaat taaaagcaca aagataatga ataatgtaat aactatagct aaattagtac
     3241 cattaatagt ttttataata gttggaatat tctttataaa atttgtaaat attgttcctt
     3301 caggaaatgt agttaactct agtatgggtc cagccattat attagtattt tacgcattta
     3361 ccggctttga atcatttatt gtggcaagtg gagaaatgga aaatcctaaa aaaaatcttc
     3421 cagtcgcatt aataaccaca atatttatat gtgcaataat ttatatatta attcaaattg
     3481 tctgtatggg tatacttgga gatagactat ttgaaaacag tattccaatt gcagatgctt
     3541 caagtgtatt tttaggcaat tatggtaagg ttttcatatc tgtagcaaca ttaatatcta
     3601 tatttggtat aaatataggt tcatcaatag taactcctaa atgtggttct tctctagcag
     3661 aagaaggttc tcttcctgca tttataggaa aaacaaataa atatggtgct ccatatgtag
     3721 caataataat atctttaatt tgttgtatac cacttgtgct aactggaagt tttgaacaac
     3781 ttgcagtaat gagtgtaatt gcaagatttg ctcagtacat accaacttgt ttatcagtaa
     3841 tagttttaag aaaaagaaca gatgttaagg cctcttttaa gattccattt ggccctgtta
     3901 taccaatagt agctatttta ggtagcttat ggttgttaca acaagcttgg gcagaagata
     3961 tttcaaaacc tataactcaa aatagagttt taataggatt aggggcaatg ttgttaatag
     4021 ctcctctata tatatttatg aaaaaaaata aagaaatcga gcataaaaat aaatcagaag
     4081 attctaaatt atatatttcg agagaaaaaa ttcaa
//
LOCUS       DAFKMX010000250         4105 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFKMX010000250 DAFKMX010000000
VERSION     DAFKMX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.257"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            381..2957
                     /locus_tag="LZB08_003486"
     CDS             381..2957
                     /locus_tag="LZB08_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7316949.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKMGQCEIFSNLNESKLSDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMVGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFMIIVLSMSIGGIIVIKDNYKYSFLDVQNKSVQEQTYM
                     NADFILSNVYLNETGNNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NKLDYYETINSKPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLIYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIIAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISEREFKNMILYEGILYGVLSSIITIVSGLI
                     IQLKMYYMQGFISHGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            2969..3640
                     /locus_tag="LZB08_003487"
     CDS             2969..3640
                     /locus_tag="LZB08_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316950.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            3667..4008
                     /locus_tag="LZB08_003488"
     CDS             3667..4008
                     /locus_tag="LZB08_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316951.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         1706 a          462 c          626 g         1311 t
ORIGIN      
        1 atatatttag tgccctccat aaataaaatc aatgcaaaac acgatataaa aaatgttaat
       61 atgcctcttt ttaatatgtc taattctttg taaaatttat acttattcat tataagattg
      121 ctaaatataa acaaagttag tattactata ggaaatacaa aattgttatc catgattccc
      181 ctcccctcta caatttatat agggattata tcattttaaa aaaattatgt aaaaagttaa
      241 aatttacata tagattaatt aaacttgtaa aaatacagat gaatttgtac aattaactac
      301 ttaaaacttg aaaatactta ctttgctata tattttagat aaatatataa ttgatttagt
      361 taagaaagga agtggtaaat atgaaagata atttgaaaat agtactaagg tatataaaaa
      421 gttataaagc taggtcttta gcaattgttg tgagtataat tcttgctaca tcactcgttg
      481 ttggtattgg taccctctca agaagtgctc agcaagcaga tgtagataga ttgaaaagag
      541 agcttggaac tgaccatgta tactttaaaa atataaagaa aaatcaatta aagtccataa
      601 aatctagcaa agatattaaa aatctgggta taacatctta ctatgcgtct acagatgtta
      661 atgaaaaatt acctataaac atactatatg caaataaaaa ttatttgact agtcattcta
      721 aacttataaa aggtcgattt ccaaaagcta gcaatgaggt ggtagtcgag aaatggattt
      781 taaatagtat gggcttaaaa ccagagataa atcaaaacct tacttttaaa ttatatcaaa
      841 aagaaaatcc agaaactttt aaagttgttg gaatccttga agatagattt atagagaaaa
      901 aaatgggaca atgtgaaatt ttttctaatc tcaatgaatc aaaactaagt gattttacaa
      961 cttatattga atttaatgaa ggcagtgata taaatacaaa catagacaat ataataaaaa
     1021 atgctatgtt agatgagaaa agtataggaa gaaactcaat gcttatagaa tctactatga
     1081 aaaacggtac tttagataat tcaagtaaat acgttgcaat tgctatgagt ctatttgctg
     1141 gtattgtaat atatagtatc tatgttatat caatatacca aagagtacag gaatatggcg
     1201 tcttaagagc tattggttcc accaacttta ggattttcaa acttatgctt tatgaacttt
     1261 tcatacttgc tctaatagca atgcctatag gtatatgtat aggtatggta ggtgcacaaa
     1321 tatttaatag gtctgtagga aatattcaat ttgaaggaaa tataaatgtt acaccttttg
     1381 ttattccaga taaaataatt ctcttatcaa ttgcatgtac aatactaata atactaatta
     1441 taagtttctt tacatactta aaaataagaa gaatttctcc tattgatgct atcagaaaaa
     1501 cttttggagc tgataaaaat gtaaccaaag ttaattattt aatatcaaaa ttaactttaa
     1561 atatgtcagt aacaaaatct atatctgcga aaaacatctt tagaaataaa aaggggttca
     1621 tgataattgt attatctatg agtataggag gtataatcgt cataaaagac aattataaat
     1681 attctttttt agatgtgcaa aacaaatccg tgcaagaaca aacatatatg aatgcagatt
     1741 ttattctatc aaatgtttat ctcaatgaaa ctggtaacaa tacttctttt aaagatataa
     1801 aagggtttag tgatagtcag atagataaaa taaaaaatat agatggaatt gacaaagtca
     1861 aaactgctag tatcttaaac acaagaatag aggtaaataa aataaataaa ttagattact
     1921 acgaaactat taattcaaaa ccttattata aactaaatcc tctgttcata aaaaataaga
     1981 ctactggcaa atatacacta aagcaaaagc taaaaggtta taatgatgag atgataaagt
     2041 cattagaaaa atacttagta agtggaaata taaacttgaa aaaaatgaaa aaagaaaatt
     2101 tagctattgt atatgtacca gaaatttcta aaacacagaa atacacaaag acttttactc
     2161 ctggtgaagg aacaccagct gtagatataa aagtaggtga tactataaaa gtcaaatatc
     2221 caaagggtgg gatggataca gggctttatg aaagtctaaa agataattat gaatacttag
     2281 aatatgaatt taaagttgga gctattataa gttatacttt tgcagataat ggtatgtact
     2341 ctggagatga tggtgtagat gtaataacga gtagcgaata cctaaaaaaa ttaactggtg
     2401 tagataaata taacttaatt tatgcagacc ttaatgaaaa tgcaaacaag aaaaaaataa
     2461 ataacctttt aggtaaaatt ggaagtgaag ttcctggaac tacaacagta gatatgatgg
     2521 tagaaaaaga aaattctgat aagatgacat ctagaactat gatttatagt tatggcataa
     2581 tagcaataat gttcataatc agtgtactta acataataaa caacataagt tataacttaa
     2641 cttcaagaac tagtgaattt ggaatgttaa gagctattgg tatctctgag agagaattta
     2701 aaaatatgat actttatgaa ggaatattat atggtgtact ctcaagtata attactatcg
     2761 tgtcaggact tataatacaa cttaaaatgt attatatgca gggctttata tcacatggat
     2821 tgggattttc aatagattac aaaatatata tactagttgt tgtagcaaat ataattgtag
     2881 gtattctagc tacatatatt ccatcaagaa agataaataa aattagtata gttgaagcta
     2941 taaacattac agaataggag ggataattat gcctatattg gaaactatta atttaggtaa
     3001 gatttatggg aaaaaggaaa catctgtaca tgcactaaag aatgcaaatt taaaaataaa
     3061 taaaggtgaa tttgttgcaa taattgggcc aagtggaagt gggaaaagta catttctaca
     3121 tttagtaggt ggtcttgaaa gaccaagtaa tggtactata aaggtagatg gtaaagatat
     3181 atgttgtctt tcagataaag aattagcaag atatagaaga caaaaggtag gatttgtctt
     3241 tcaacagtat aacttaattc ctgtattaaa tgtaaaggaa aatatagaat taccactaaa
     3301 gttagacaat aaaaaaatag ataaagaata tattgatgac cttataaatt tattgggtct
     3361 taatgaaagg aaaaatcatc ttcccaacca attatctggt ggtcaacagc aaagagttgc
     3421 tattgcacgt gctttatctg caaaaccaag tataatccta gcagatgaac caacaggaaa
     3481 tttggacagt aaaactacag aagaagtcat ggatttacta aaaagttcta taaaaaaata
     3541 taaccaaaca cttataataa taacacataa tgaaaatatt gctagaaaag cagatagaat
     3601 tatatctata atagatggag agcttaaatt aacactctaa aactatttat agaaaagagg
     3661 atttaaatgt tattgatttt aggtaattta aatttaaact tggattttga ctacagaata
     3721 ataagagaag aaaatgatga tgtagatata tttatagata ttaattatcg tagcttagat
     3781 atagatactg atggttctaa cttatttaat tctagaatac aatttccatt tgtaagagca
     3841 ttgattctaa ggctaaataa gaacaatcaa tgtatgacaa ttcatttgtt aagagatata
     3901 gatttattct ctgcttttgc caactttgaa gtggattata ctgactctat aataaatata
     3961 aaaaatcaaa atgagaaagt aattttaaat aaatctatca aaaaatagta agggggcctt
     4021 aataggcccc aatttttttc ttttctaaac ttattatatc atgataaata aattagtcaa
     4081 tagaattttc tgaatatttt tataa
//
LOCUS       DAFKMX010000251         4076 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFKMX010000251 DAFKMX010000000
VERSION     DAFKMX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.258"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(87..2024)
                     /locus_tag="LZB08_003489"
     CDS             complement(87..2024)
                     /locus_tag="LZB08_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316952.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(2011..2778)
                     /locus_tag="LZB08_003490"
     CDS             complement(2011..2778)
                     /locus_tag="LZB08_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316953.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            3066..3740
                     /locus_tag="LZB08_003491"
     CDS             3066..3740
                     /locus_tag="LZB08_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316954.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            3764..>4076
                     /locus_tag="LZB08_003492"
     CDS             3764..>4076
                     /locus_tag="LZB08_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316955.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFVSYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQ"
BASE COUNT         1502 a          549 c          415 g         1610 t
ORIGIN      
        1 aaactcaatt ctagtatttt ctatctgcac ttaaataagt tattttattc aaaacatata
       61 tctaattctt ttgacttaaa aataatttat tcttttatca tgtttagagc atattttaaa
      121 gttaaaaaat aatatataaa atatcctata aaataaatga ctagagttat gataattggt
      181 acaagtaatg accttggtat aaacttttgt gctatagaca tagcaaataa attatgaact
      241 gtacctacaa ctaatggcat taaaaatata acttttaact gttttgatat tattttctca
      301 atatctttat tatttgctcc aagttttatt aacataatat atctttcctt atcatcatat
      361 atatttgata attgtttaaa taagattata ctacctgtac ataaaagaaa tactacagac
      421 aaaaatatgc ctataaacag cattagacca cttaatttta tcaagttatt ataactttca
      481 atacttgatg taaaattaaa aggataagta aatttcatat tttcacttac tatatttttt
      541 aattttaaag tcaaatccag ttcttttttt tcatttttta tatcaattaa tcttattaat
      601 ctagaatttc catgtaattt taactgatta aatacgtcat cttttactac aattaaatca
      661 aacgtagctt gtgaatttat ttctggctcc aagatgcttt tttgtacttt aaacagattt
      721 atatcatact catttgtatc tttaatatgc aagtttattt tttcaatctc aaagtcttta
      781 aaaaatattt ttttgtatga atcagaaaca tagtacacag attcctttga agttaattct
      841 tcataattgt tattctgata ttgtcttaat tttttaaaat cactttctct aataatatct
      901 acagacgttc tatcttctat ataactattt ccatttttat aatacatttc aatatttata
      961 tctttgtcta taagttctat tgttttatca aagattatct tattatcttt tttatattta
     1021 tataataatt tgtcaatttc gttatttaca taagaatttc ctaaatttat ggtataactg
     1081 tatttataat tttcacttat gttcctatct atatcgtaat aaagtgatat ggtaaaccct
     1141 agcatagtta cacttgtagc tattaatatt gctattacag ccaatacact actattgctc
     1201 tttaatttaa atccaagttc agaaaatgca attaaattct gtcccttata ataaaaactt
     1261 tttctcttct taacagcatc aataatcata tacatcgttg atgaaaaaaa taaaaaagtt
     1321 ccaggtatta ttgtaactaa tataaaaact gaaagcataa tattttcagc aataaaataa
     1381 ctaattgaca ttaaataaga aatagctatt aataaaacac caagtacaca ttttaacttt
     1441 ataaattttt taaacttata cttttctgga tatttattaa ataactctat aattctctta
     1501 tttctgataa ctatagtatt tcttattcca ataactataa atattattaa aaataccgct
     1561 agtgtctgaa ctaaggcctt tatacttaag ttcatattta ctactatcat ttcattcatt
     1621 aattttacaa gcatcatagt cataagcttt gaaaataaca atcctaaaaa tatacctatt
     1681 aatattgcaa ctatacccat caaaaaagtt tcatataaaa atatttttcc tatctgattt
     1741 ttcctcattc caagtaaact ataagttcct acttctcgcc tacgtctatt gaaaaaaaat
     1801 gagtttgaat aatatataaa taaaaataca aataccacta ttacaattga acttgtatta
     1861 atactcatcc tagttttctc tccaagtgaa ctcaacgctt cattatactc tatagattta
     1921 aaactaaaaa atacaaatac actaaataca attgaaataa agtacattaa ataattaaaa
     1981 aaactattct ttacattttg cattgcaatt ctaaataaag tcatttttat taactcctcc
     2041 aattaaggaa actgtattta ctattttatt atagaactct ctgttacttt ctccctttac
     2101 aatttctgtg aatattattc catcctttat aaagagtatt ctattgcaaa aactcgcact
     2161 tatagcatca tgagttacca tcaatattgt tttattgctg tctttgttta attttaatag
     2221 acaattcaac aaatccctag cagatttaga atctaatgct cctgtaggct catctgcaag
     2281 tactaatgat ggttttgttg ctatagctct acatacagct cctctttgct tttgtccacc
     2341 agaaacttcg ccagggtatt tatctaatat agattctata tttaattctt ttgaaatctc
     2401 ttttattcta ttttctatta ctgatactct ttcatttttt aaagaaagag gcaacattat
     2461 attttctttt attgtaagtg tatccaaaag attagaatct tgaaatacaa agcctagatt
     2521 atcacgtcta aaatctgcca attcattttg cttcatttta atagtattct ttccatttat
     2581 ataaatatct cctgatgaag gtaaatctat tgtagaaata atatttaaaa gtgttgattt
     2641 accagcaccg gaagacccca taataccaac aaattctcct ttataaacct tgaaagttat
     2701 tcctcttaga gcttcatgtt ttgaaccttt tcttccatac tcctttttta tattttcaac
     2761 agataatatt tcttccatag ttaataatca cctttcatca caattagtta tttatcaatt
     2821 ctcacaaata tattatatat attaaaatgg cttttaaaca tttaattata ttaatttaag
     2881 gttacatttt cgtaagtatt aattatattt cttcaaattt attgcaatta tgtttaaaat
     2941 ataatttatc aagtaaaata tagttatatt aatataatta ttttaggtaa taaggtagaa
     3001 atattttaat ttatcaagta gaatatagtt atgttaatat aactattttg aattaggagg
     3061 taagaatgtt taaaataatg gtagttgaag atgacgtctc attgaaaaat atcatagcaa
     3121 agtgtcttac taaatgggga catgatgttc atcaaataga aaatcttgaa aatataatag
     3181 aagagttcaa aaactataac ccagaattag ttttattgga cataaacctt ccattttatg
     3241 atggttttca ttggtgtaat gaaataagaa aaatttcaaa agttcctata atttttattt
     3301 cttcaagaaa ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca gatgattata
     3361 tacaaaaacc tttttctgta gatgtcttag tagctaaagt taatgccctt ttaagaagaa
     3421 cttataattt tgttgataat aactcaaatc agattattca caatggtgtt actttagatt
     3481 tatcaactgc aacaataaac tatgaagata atactattga gctaacaaaa aatgaaatca
     3541 aaattctcca tgagcttatg aaatataagg ggcagatagt aagtaggaat aaactcatga
     3601 aaaaattatg ggataatgat tggtttgtag atgataatac attaactgtc aatgtaaata
     3661 gaatacgctc aaaattaaat gaaattggtt tagaagattt tatagaaaca aaaagaggat
     3721 tgggttatat aatcagttaa aacaattcta agtaggtgaa tttatgagct ttaaagattt
     3781 tctcaaggat aaactaggtt ttttagtata caattttact ttcttaattt ttacagtatc
     3841 agttattata ttttctcctg tagatgtttt tctgactgat actattttat atatattagt
     3901 tgtaaatgtt gtgtttttat ttttatatct atttgttagc tacatcaaga agaacaaatt
     3961 tttagataag ataaaaaatg atacattcaa aattaatatg aatgatatag aattagctag
     4021 atgtaaaaat gaagaaaaaa tatacttgaa cattattaaa aatcatcaat atcaat
//
LOCUS       DAFKMX010000252         4074 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFKMX010000252 DAFKMX010000000
VERSION     DAFKMX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.259"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     misc_binding    159..370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            533..1822
                     /locus_tag="LZB08_003493"
     CDS             533..1822
                     /locus_tag="LZB08_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBM7316956.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1876..2955
                     /locus_tag="LZB08_003494"
     CDS             1876..2955
                     /locus_tag="LZB08_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBM7316957.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2976..3341
                     /locus_tag="LZB08_003495"
                     /pseudo
     CDS             2976..3341
                     /locus_tag="LZB08_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            3990..>4074
                     /locus_tag="LZB08_003496"
     CDS             3990..>4074
                     /locus_tag="LZB08_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7316958.1"
                     /translation="MIAVEKAYKFRVYPNKKQQELINKTFGC"
BASE COUNT         1509 a          456 c          797 g         1312 t
ORIGIN      
        1 tattttgatg aaatttaaaa aaatacatta tataaatata gtaatagtat gaaaatttac
       61 atataaaact aaaaaatgca agtaattgac tttttaagtt gacaaaatat tgcatatata
      121 ttatgatata aatcataaat taactacaaa tcaatattat atattcgtag aagaagaaag
      181 tagcataaag gaattttaca gagagttaga tttggctgag aactaatatt ggaatttatg
      241 tgaaaaggac tttggagaat actattgtaa ttatgttaga atagtggttt aattcgcctt
      301 aagaaaatga gtgagtaaaa ttaattttta ctaataagag tggtaacacg gatttatatt
      361 cgtctcttgg atttttccaa gaggcgtttt ttattataaa cttatttcag aaaatttaaa
      421 actatatatt aaatgaaaat tataaaaata tgatagttaa taaatttata aattagaaaa
      481 ttgtaaaata tgctaaggtc agtagtttta cgatttaaat ggagggatga aaatggaaga
      541 agaaaaaatt aactatgctc tggaacagat tccagagaat caaaaacgtg gttgggtagc
      601 tatgttttca gttttagtag caataggtgt agatttatca tctgttattt taggcgctga
      661 gttggcacag agtatgccaa tgaaacaagc aatattgtca gtaattgttg gttcattttt
      721 ttctgcaatt ttgtatacta catgctcttt ggttggttca tctacaagtt tatctacatc
      781 tatgataaca aagtatgtat ttggagaagc tggagcaaaa atattttctc tagttatagg
      841 ggtttctcta ttaggttggt ttggagttca agtaggtttc tttgctcaaa atgcacagat
      901 aataataaaa gatatattta atttagatgt cagtatgcag attttaagcc taataggtgg
      961 tctcttgatg atgagtactg caatttatgg atataaggca atggagaagt tgagtgtgta
     1021 ttcagtacca tttttattag ttcttatgat gctaactata ttcttagctt ttagagcaaa
     1081 tggaatttca gttgatgata atatgaaatc tactatgaca tttgcaggtg gagtttctct
     1141 gtcaatgagt ataataatag ttggtgcaat tgtatctcct gatataagtc gttgggctaa
     1201 aagtagaaga gactgtgctc tttcaagctt tttgggaata caatttggaa atgcttttat
     1261 gataatagtt tcgatagtat tagtaaaatg tatgggaact tcagatatta tgagaatttt
     1321 tattacatta ggaattgcta taccaggtat aatagtactt acattagcac aatggactac
     1381 aaatacaagt aatgtatatt cagcatcatt aagcatagct cttgtactta agaaggctcc
     1441 tgagaaagta ttaactatag tccttggtat tatagcgaca ttattggctg tatttggaat
     1501 atatgaaggt tttataggat ttttaaattt acttggaatt gtaattgcac ctgttggtgg
     1561 tgtatataca gcagaatatt acatagtaaa gcaagaactt aaaggctttg ataaaggagt
     1621 attatataaa ccaatagtta aacgttcaat agtgtcttgg ataattggta tattaataac
     1681 ttacctttca acatatggat ttataactct tactactata gcacctttag atggttttat
     1741 agcagggttt gtagtacaat ctattattgg aaaagtgctt tgttcaacaa aaaataagca
     1801 agaaatagac aaagcagtat aaagaaattt gtttataaag tataaaataa attagaaata
     1861 tgaggagaaa gtaacatgag atttttaaca gaagaaagtg tagataaaat agcagttggt
     1921 gcagcagtgc ttggaactgg aggtggaggt gacccatatg taggtaaatt ggttgcaaaa
     1981 caagccatta aaaagtatgg tcctgtaaaa gtaataagtt tagatgaact tgatgatgat
     2041 gctttggtag ttccagtatc tggtatgggt tctcctgtaa ttacaataga aaaattactt
     2101 tcagaagtag agttaactac ccctttagaa ataatggaga aattattaaa tagaaaagtg
     2161 gatgttataa ttcctataga aataggaggg ataaactcac ttatgcctat agcagttgcc
     2221 gctaaaaaag gacttcctat cttagatgca gattctatgg gtagagcttt ccctgaagct
     2281 caaatggtaa ctttttatct tgaaggatat gaggcatctc ctgccgtaat gtctgatgaa
     2341 aaaggtaatt ctgccatcct ttatcctgtg gatggaattt ggtcagaaag attagctaga
     2401 actttaactg tagaaatggg aggaagttct tctattagtg actataattt aagtggagct
     2461 caagttaaaa aagctgctat aggtaatact cttacaatag ctgaaactat aggagggttt
     2521 ttacttgaaa ataaatctga ttcaaaaggt gctgttgaga atatactaaa agaattgtgt
     2581 ggatacaagt tatttgaagg aaaagtaatt gatattaaga gagagttgaa aggtggattt
     2641 actagaggac atgccttttt tgctgggatt aatgagtatg atggagagta ttctatatta
     2701 ttccaaaatg agaatttaat tgccaaaaaa ggtgatacac cactttgtat tacaccagat
     2761 ttaatagcag tattagattt agaaactgga tttccaataa ctacagagag aataaaatat
     2821 ggctctcgtg ttatggtagt agcttttcct tgtaatgaaa agtggagaac agaaaaagga
     2881 attgagacag ttggacctgg atactttgga tatgatgtgg aatataaaac agtagaagaa
     2941 ttacaaggaa aataaaagtt taggaggaaa ttttgatgta tcgtgtagga attgacgttg
     3001 gaggaacaaa tacagatgct tgtatactgg atggggaatt aaaagtaatt cattcagtta
     3061 aggtggcaac tacaaaagat gttgaaactg gtgtttacaa tgccttaaaa aaagttatag
     3121 atgaaagtaa gattgaccat tcattaataa aatatgctat gcttggtaca actcattgta
     3181 caaatgctat tgtagaaaga aaaagactta ataatgtggc tatgataaga ataggaaagc
     3241 cagccactac tgccattctt ccatttatag attggccaga agacttaaga gaaaaaatag
     3301 aattagattc aatattggta tctggtggat atgagtttga ttaaaaaata gctacgcaca
     3361 ctcgtgactt tagtcatgag ttagtagcta aaatagtcag catataggga aacctatatg
     3421 tagagatagg atagaaccta tccaatacta cttgaattgc tggaaacccc taaagctaac
     3481 caaactaaaa cataaggatg aaataaacct aagtgtgaaa gttacgaaag tagaaaaaat
     3541 tggttagatg gtataaggtt aaaccctaag tactttaaaa tgggcaatca gcaggtaaac
     3601 tccgaatagg agaaacttca acgactattc ctcttgaggg aagtacacta caagctattg
     3661 gtagtggaag tgggtagacc ttaacggata atgccgaagg ataagatata gtctgtgctt
     3721 acatgaaagt gtaagaagtt cataagagaa ctgcataggt agtagcgaac ttatgtgaac
     3781 gacaacctca aaaatgataa aagttaagac ttacggttct taaatatgtt atttaaaatt
     3841 aaatataaaa ccttctaaat aattgtttta gataattaca tttgttaaca ttattatagg
     3901 tgattaataa attgttcacc acagcattat ataatatatt aaatatgata aaatcattaa
     3961 aaagtgtatc aaaaaacaat taaagtgagg tgatagcagt ggaaaaagct tataagttta
     4021 gagtgtatcc aaataaaaaa caacaagagt taattaacaa aacttttgga tgtt
//
LOCUS       DAFKMX010000253         4064 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFKMX010000253 DAFKMX010000000
VERSION     DAFKMX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.260"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            37..957
                     /locus_tag="LZB08_003497"
     CDS             37..957
                     /locus_tag="LZB08_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBM7316959.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1072..2229
                     /locus_tag="LZB08_003498"
     CDS             1072..2229
                     /locus_tag="LZB08_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBM7316960.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2314..3813)
                     /locus_tag="LZB08_003499"
     CDS             complement(2314..3813)
                     /locus_tag="LZB08_003499"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBM7316961.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         1598 a          559 c          620 g         1287 t
ORIGIN      
        1 ttaataatat tagtgaaaat gaaaggatta taaaatatgt ataaatcaga aaatttttca
       61 aaaaaatctg tattaataaa ttcattttta agtaaatttt taaaaataat tggatggggt
      121 ttggtaagct tatttacatt atcagcctta gtactttcaa ctatttccat tataattgga
      181 tgggctgtaa tacttcctat tcaagtatgt gcagtatttt tacttatagg aggagtttgt
      241 atttttaaag gtaagagatt aggagaccaa atttctcgtt ataaaaaata ttgtacaatt
      301 ataaacaata gaaatattat tccagtagaa cttattgcag aaaacacatc taaatcatta
      361 aattttataa taaaagatgt acaaaagatg atagacaaaa actactttat aaatacttat
      421 attgataaaa gaaataatca gattgtgtta acaaatgaag attttattcc accaagatat
      481 gaagatgtag catatgaagt caaagaagaa gaaaagactg atgaatccaa tgaagttgat
      541 acaattataa ataaaggaat gaattatcta acacaaataa aagaagctaa taaaaatatt
      601 aaatcagaat caatgggcga taaaattgta caagtagaag atgttacaag taaaatattt
      661 gatgttgtaa aacatgaccc atcaaaactt actcagatac aaaaatttat ggattattat
      721 cttccaacca ctttaaaact attaaattca tatcatacat tggaagaaca aggaattgat
      781 agagaaaata taacaacaac tatggaaagt attgaaaata caatgaatac gattgtagta
      841 gcttttgaaa atcaattgga ctatctgttt gaagatgaag ccattgatat ttctacagat
      901 attactgttt tagagaatat gttagtccaa gaaggattaa cgagtggtaa attttaactt
      961 caatagaaaa aatattctgt tatttttaaa caattaatta gtcataaagt ataaaatgtg
     1021 tattatataa gttaatcagt actttatgaa ggaagaggag agaaagatac aatgaataat
     1081 ttagatgata taccagttat gccaactttg acacttgacc catttggaga agaatcaaac
     1141 actaatgata tagataattc tgacttatta atgaaaaagg atgaaaaaga cccagaagaa
     1201 gaaaaactgt ctgaatctga acgcaagatg gtaaaggaat ttgcggaaaa aatagatatt
     1261 acaaatacaa atatgatttt gcaatatgga gctggtgctc aaaaaaaggt agcaagtttt
     1321 tctgaaacag ctttaaagag tgtaaaaaca agagatttag gagaagttgg agatatgctt
     1381 acaaatctag ttactgattt aaaagcattt tcagcagatg aaaaagaaca aagtggtttt
     1441 ttaggaattt ttaaaaaggc aaataataaa atttcaaatt taaaaactaa gtatgatagt
     1501 gcagaggtaa atgtggataa agtaagcaaa gagcttcaaa agcatcaggt taaaatattg
     1561 aaagatatag caatgcttga taagatgtat gaattgaact tagcttactc aaaagagctt
     1621 actatgtata ttatagcagg aaaacaaaaa ttaaaggata tgaaagaata tgagatgcca
     1681 aagttgagag aaaaagctcg tttatctggt tcaacagaag atgctcaatc tgtaaatgat
     1741 atggtttcac tttgtgacag atttgaaaag aagattcatg acttagaatt aactagaatg
     1801 gtatcgcttc aaatggctcc tcagataaga ttggttcaaa atagtaataa tctgatggca
     1861 gaaaagattc aatcaactat agtaaatacc attccattat ggaaaaatca aatagtgctt
     1921 gctcttggaa tttctcactt aaatcaagct atgaaagcac agcgtgaagt tagtaatatg
     1981 acaaatgagc ttttattaaa gaatgcagaa actttgaaga tgggaacaat agaaacagca
     2041 aaagaatcag aaagaggaat tgtagatata gacacaatta agaaaactaa tcaatctcta
     2101 atatctacta ttgatgaggt agttaaaata caacatgatg gtagaataaa gagacaagaa
     2161 gcagaagttg aattatcaaa aatagaaaat gaacttaaaa gtaagcttct tgaatttaca
     2221 gttaaataaa aattatacta atataaaaag agactgtctc aaaatagaga taagtctctt
     2281 tttcattgtt taaatgatta aagtatattt atattataaa tttctattat cttttaacat
     2341 aacatcatga gctccacctt caactatact agtagaagat acaagtgtaa gtttagcatt
     2401 tttttgaagt tcaggaatgc taagtgctcc acagttacac atagtagaac gaactttact
     2461 taaagaaaga gttacattgt ctttcaaaga accagcataa ggcacataag agtcaacacc
     2521 ttcttcaaaa gaaagtttct tatctccacc aaggtcatat ctttgccagt ttctagcacg
     2581 agcagaacct tctccccaat actctttcat ataactacca tttatattaa ctttgttagt
     2641 tggactctca tcaaaacgag agaagtatct accaagcatt ataaaatctg cacccatagc
     2701 taaagctaaa gttatatgat ggtcataaac gataccacca tcagagcaaa ttggaatata
     2761 aatacctgtt ttttcaaagt attcgtctct agcttgagca acttcaatta tagaagtagc
     2821 ttgtccacgt cctatacctt tttgttcacg agttatacat atagaaccac caccaatacc
     2881 aactttaacg aagtcagcac cagcttcagc taggtaaagg aatccttcgc ggtcaactac
     2941 atttccagct cctaccttaa ctgtatcacc atatttttca cgaatgaaat caagtgttat
     3001 tttttgccac tcagaaaaac cttctgaaga atctatacat aaaacatcag caccagcttc
     3061 aactaaagca ggaacacgtt cagcaaagtc tctagtatta ataccagctc caactacata
     3121 tctcttagaa gaatctaaaa gttcaagtgg attttcttta tgagaagagt aatctttacg
     3181 gaataccata tacataagat taccattatc atcaagtaca ggaagagaat taagtttgtg
     3241 gtcccatata atgttgtttg cttcctttaa agtaacatct ttgttagcat aaactataga
     3301 tgacatagga gtcataaatt cactaacttt agtatctaaa tccatacgac ttatacgata
     3361 atcacgactt gcaacaatac ccaataattt accagttgaa gttccatctt cagtaacagc
     3421 tactgtagaa tgtccagttt tttcttttaa gtcaagtatg tcttttaatg tgttatcagg
     3481 tttaatattt gaatcactta caacaaaacc agctttgtgg gatttaacac gtgctaccat
     3541 agcagcttca ttttcaatag tttgagaacc gtatataaat gaaataccac cttctttagc
     3601 aagtgcaata gccattttgt catcagaaac agattgcata atagcagaag tcaaaggtat
     3661 attcatataa atatctgctt cctcgccctt tttaaattta gtaacaggtg tttttaaact
     3721 tacattagca ggtatacaat cagtagaaga gaaacctggt acaagaaggt attcactgaa
     3781 agtatgtgat ggtgtatcaa aataaaacgc cattttttaa tcactcctta tatttttata
     3841 ctttttataa ttgatagtat taattataac ttattcttta attttattaa agtattttgt
     3901 caaatattga gatttttgaa aaatttaaac tattttaatt aaatttgttt aatatatatt
     3961 gccttatagg gtaaagttgt atattaaata atgtttagtg tttaatagta gctaatttaa
     4021 aattaatctt tagtgattca tataattaca tttgtatagt ttac
//
LOCUS       DAFKMX010000254         3945 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFKMX010000254 DAFKMX010000000
VERSION     DAFKMX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.261"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            641..1555
                     /gene="queG"
                     /locus_tag="LZB08_003500"
     CDS             641..1555
                     /gene="queG"
                     /locus_tag="LZB08_003500"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBM7316962.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRNIIKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIKNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            1661..3898
                     /locus_tag="LZB08_003501"
     CDS             1661..3898
                     /locus_tag="LZB08_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7316963.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         1717 a          320 c          754 g         1154 t
ORIGIN      
        1 taatattaat aagtataagt ttagtaaaga gagtaaaaag aaatccatat aagtcaagta
       61 aagacttaat aaatggcaat gtaaatggga tattaattat aagaaaaaat caatcttttt
      121 gcttaactat agaaaaaatg tcaaaatata actaatgata tttgaagttt tagagtttat
      181 attaaaaagt aagaaggtta agtgggtaaa aaaggttgaa tagattaaaa aggctaggta
      241 tctaaaagag ttaagtaggt aaaaaagtta aataagtaaa tgaaaaaggc taagtatcta
      301 aaagagttag taggtaaaaa agttaaataa gtaaatgaaa aaggctaagt atctaaaaga
      361 gttagtaggt aaaaaactaa ataggtaaaa agatgaagta tctaaaagag ttagtaggta
      421 aaaaattaaa taggtaaaaa gacgaagtac ctaaaagagt tagtaggtaa aaaaattaaa
      481 tagctaaaaa gacgaagtat ctaaaagagt taagcaggta agaaaattga atagctaaaa
      541 agattaagta tttaaaagag ttagtagcta gaaaaggtta aaaagactaa aaaaattaaa
      601 taaatttaaa aagtcaatta gattaaaaag gaataatttt atggataata aggaattaaa
      661 agcattttgt aatttaatag gattaaactg tgtaggagta gcaggggtag ataagtatga
      721 taatttagaa aagatactta aggacagaca acaaaaaggt tatttaactg gtatggaaga
      781 accaataatt gagaacagaa ttaacccaag aaatataatt aaggatgcta actctataat
      841 agtctgtgca tttccatatc atataaatag acaagaaaag aataaaaatt caaatttatc
      901 aaaatattgt catggaaagg attatcacat tgtagtaaag gactttttac aacaaatttg
      961 tgattatatt tctaaaacag taacagattt taaatacaag ttatttgctg ataatggacc
     1021 attagttgac aggtatttag cttatttatc aggaatagga tattttggaa taaataataa
     1081 tatcataact gatgaatatg gttcttacgt atttataggg tatatagtaa ataattataa
     1141 atttgaagaa gataaaccac tagaaaaaag ttgttttaaa tgtggtaagt gtgtaaaata
     1201 ttgtccggga aatgctatat tgggaaatta tgatatgaac cccaaaaaat gtttatcata
     1261 tataactcaa aaaaaaggag atttgtcaga gaaagaaaaa gttgttttaa agaatggcaa
     1321 aaaggtattt ggatgcgata tttgtcaaga agtatgtcca cataatgttg aaataccaac
     1381 aactcatatt ttggaattta aagaaaatat aatagattat ttgaattatg atgagataaa
     1441 aaatatttca aataaagaat ttaaaaggag atatggagat agagctttta gctggagagg
     1501 gagaaatata ataaagagaa atatggaaat aattttagat aagtatgatg actaataata
     1561 agagtctgtt tcatatactt taatgtgtat ttactttaac acttgttgtg taaaagtcat
     1621 attatatcat taaagttaaa tatggggagg tgggggaata ttgaaatata ataatacatc
     1681 tgaaataaat ccactagatt atatattggg tcaagaaaga gctgtagaag ctatggaatt
     1741 agggttgaaa ataaataatc cagcgtacaa catatatata gctggtgaac ctggaactgg
     1801 taaaagtact tatgccttaa aggttttaaa tgagtatgca tccaaaaaaa atacgcataa
     1861 agattggtgt tatatatata attttgaaaa ccctagagag cctattatag ttgaattaga
     1921 aaaggggttt ggaagagaac ttaagaaaga tatggagaaa ttaattgaaa gtcttttaga
     1981 agattttaaa aatgcgtttg aaagtgaaaa ttttgaaata gaaaaaaata aacttctgga
     2041 tgaatatgaa atagaaaaag atatgctttt gaagcaaatt aagaaatatg gagaagaaaa
     2101 aggatttaaa ttaaaacaga gtaaaatggg aatagttttt atccctttga aagaagagga
     2161 agatgaaagt gatgagtctg atgaagagtt ttacaaagct aaaagagaac tagaaaatat
     2221 ggcgatacag gtcgtatata aaattagaaa cttagaagaa atagctgaaa aagcagtgtt
     2281 agagcttgaa gaagagatag ctaaacttgt agtagaacct catatcaaga gattgtgtga
     2341 aaaatatgaa aattatgata agattcaaac ttatctggaa aatataaaga aagatataat
     2401 agaatatatg tatttattct atttagatga agaggagtta aaagataagt atgataaaga
     2461 gcattttatt aaatataaaa ttaacctatt tgtagacaat ggaagctcaa agaataaaga
     2521 atcagcacct gttgtagtag agataaaccc aagtccagca aatctatttg gaaaggcaga
     2581 gtatgattat gcaaatggaa atataaagac tgatttcaca aaactattat caggagcatt
     2641 tcatagggct aatggtggtt atttagtact ttatgcagac caattactaa aatatacaat
     2701 gtcttgggaa attcttaaaa agactttgca gactaaaaaa gtaatattgg aaacacaaac
     2761 agcaattaaa ccagaaaata tgccattgga tgttaaatta gtactgattg gaagccacta
     2821 tatatatgat attttatata gatatgatga agattttgaa aaatatttta aagtatttgt
     2881 agactttgat agtgaaatgg ataaaaatga agataatgaa gaaggaatag caagatttat
     2941 agcataccaa tgtgataaga ataatttaag gcattttact aaatcagcag ttgaagaggt
     3001 tattaaattt agcactagat tatctggtga tttagaaaaa ttatcaacta agtttaataa
     3061 aataatggaa gttgtaatag aaggaagtgc ttatgcagag tttagaaatt cggagtatac
     3121 agagcagtgt gatgttaaaa aagcaataag tgaaaagaga aaaagaatca atagagtgga
     3181 aacacatatg gatgaatcaa ttgaaaatgg atttactcta atagaaacag agggaagaaa
     3241 agtaggtgtg ataaatggtt tgtcagtctt aagtacagga gaatactctt ttggtagagc
     3301 ttcaaggatt acagcgacca ctagcccagg aagtaaaggc atagtaaata tagaaagaga
     3361 agtaaatatg agtggctcta tacataacaa aggagtactt atattaggtg gctatctttc
     3421 tgagaatttt gcacaggatt tactattgtc actaaatgca tatgtttgtt ttgaacaaaa
     3481 ttatggtgga atagatggag atagtgcatc ttctgcagaa ttatatgtac tattatcatc
     3541 tcttagtgga gtatcaatta aacaaaatat tgctgttaca ggttctataa atcaaaaggg
     3601 tgatatacaa gtagttggtg gtataagtga aaagatagaa ggattttatt ctatatgtaa
     3661 aactaaaggg ttaaatggtg agcaaggcgt cataattcct agaaaaaatc aaagaaatct
     3721 cgttttatca gatgaagtta atgatgcagt gcgagatgga aaatttaaga tatatactgt
     3781 tgaaagagta gaagaggcta tagagatatt gacagatgtt aaatttgaag aaataaagaa
     3841 attggtaaaa gaaaaattaa tatctttcag caagatacaa actgtgtcaa aagaataagt
     3901 ttattataga atttaatggg tatttatgta taataaaaag caaga
//
LOCUS       DAFKMX010000255         3937 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFKMX010000255 DAFKMX010000000
VERSION     DAFKMX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.262"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            70..408
                     /locus_tag="LZB08_003502"
     CDS             70..408
                     /locus_tag="LZB08_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBM7316964.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(814..2250)
                     /gene="miaB"
                     /locus_tag="LZB08_003503"
     CDS             complement(814..2250)
                     /gene="miaB"
                     /locus_tag="LZB08_003503"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBM7316965.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2498..3934)
                     /locus_tag="LZB08_003504"
     CDS             complement(2498..3934)
                     /locus_tag="LZB08_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBM7316966.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1254 a          641 c          429 g         1613 t
ORIGIN      
        1 ttaatgtgcc tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag
       61 gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta
      121 agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt
      181 aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact
      241 tttattgtct ctataatgct atttattatg gcgtttttgg gatatagaat agagagtggt
      301 caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc
      361 agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt
      421 tgagcagtta attataaaag attgactgct ttttcatgta tattgactac tcttattaaa
      481 tattttaaca tttaaaatct atgtaaaaat aatgaatata gtacaaactt ctaataatga
      541 ttttaccttt gtggttacag tccactatat ataatttaat atgataacag ggaattacta
      601 atgacaacta ggtaagcttt tataaaatat aataaaacaa aaatagatgt acaatgaaat
      661 tatatcttat atcaaattta tttttattaa tagcaaaaac ttacattagc ataacatatg
      721 acaaattcgc ttaaaataaa aatttttaat ataatttgtt ctatagtaca tctattttga
      781 ttagtttaca taattttatt attttgtaca atattaatta tttactaata tcccatttaa
      841 tgaaaaagtt ctaggttcag taattttgac cataactagc ttaccaatta aatcatcatt
      901 tccaccttcg aaatttacga gtttattttg tctagttcta ccagcaaact tattttcatt
      961 atttttactt cttccttcaa ctagaacttc aactattcta tctttatatc catcatttat
     1021 ttctgcagaa atcctattta cagcttctaa caccctatta aatctatcat gctttatatc
     1081 ttctggtatt tgattttcca ttttagcagc aggagttccc tgtcttttag aataaataaa
     1141 tgtaaaagct gaatcatatc taactttttc aaccacatct aaagtgtcca ataaatcttc
     1201 ttctgtttct cctgggaaac caatcattaa gtctgtagaa aaagcaatac ctggaacttc
     1261 ctttttagct ttttctatta tttctaaata atattccttt gtataatgtc tattcatttt
     1321 tttcaaaagg ctactgcttc cacattgtat tggtagatgt aaaaactcgc acaccttatc
     1381 acaatctcta attgcgtata ttacttcatc agatatatct tttggatgag atgtcataaa
     1441 tcgaaccctt tcaattcctt ctatatcatt cacttttctt aaaagttctg agaatgaaac
     1501 tggattttca agtgtcttac cataagaatc tacattttgt ccaagtaaag tgatttcttt
     1561 cgttccattt gcaacaagct cttttatctc gttaattata tcttcaggag ttctacttct
     1621 ttctcttcct cttgtgtatg ggactataca atatgtgcag aaattattgc atccatacat
     1681 tatatttaca aatgctttta attcaaattt tctattactt ctaagacctt ctattacttc
     1741 tccatccaca tcccatacat ccactaacat tttgtcagaa tttattgatt ctgtaagtaa
     1801 ttgcggaaat ttatatagat tatgtgttcc aaatataagg tctacatgtt tatatttttt
     1861 tcttaattcc tctacaacat gtggttgttg catcatacat ccacatacag ctattttcat
     1921 atctggattt tttcctttta gatgttttaa ttgtcctaag ttaccataca cttttagctc
     1981 tgcattttct ctaactgcac aggtgttata tattattaaa tcactttctt caaccatcat
     2041 tgacatttgg tatcccatct cttctaacat tgaacataat ttttctgaat catgttcatt
     2101 catttgacac ccgaaggttt gagtaaacac gagttttctc tttccagttc tgttgaagta
     2161 ttcttcattt tgtctgtgta tttgttctat atatttatct tgttcttgta ttttctctat
     2221 aggtattgtt acttgttttc ttttgctcac gtctattcct cctagttaat ttcttatatt
     2281 acacataacc ttaattatat caaataagaa aaatctttta aagatattat tatttttcta
     2341 gttgtgtttt aatagtaaat ctaatgattt atatatacta tatttctaaa atataaaatc
     2401 atataattat tataataata aaaagatgtt ttaaattaaa acttaaaaca cctttcctta
     2461 aataaaatat atgatttact attaaataca attacatcta ttcattttca attaaataac
     2521 aatcatttaa tattaaatat tgaccttcct caaccgttat atatcttctt cctgaaaaag
     2581 tatcattagt aactattccc tctgcattac ctaagctatc atttctaact tcataatatg
     2641 catcagaaga atttgacatt ataatatatt ctccagcagg aatttctaat ccaattttat
     2701 acataccatt tccaaactta ccattcttgg ccttctgggc tttagcatac tttgctaatg
     2761 tcattgttga atcctcaatt ttaatatatt gaccattctt taaggtgata tatctagtcc
     2821 cactaaaaat atcatttgaa agtatactat cagcattacc tgtactatca cttgtaactt
     2881 cataataaga agcataactt cctgagtttg aagttattaa gtattcacct gctgagatat
     2941 cttttccaac cttatacatt ccatctttta tagtctttgt agtaggttta acatttttta
     3001 caaccccagt attaatatct ccaattccat ttgttatatt taaacactgt tgtactatat
     3061 ttgaatctat atatcctata gaagttatct tcttagcatt ttttaaaatg cttttattgc
     3121 taccatcatt aactaaaacc attggaccat gttttgataa agaagatcct acaagtgcat
     3181 ttgttaattc ataagctcca gctatataaa actctctagc atccttataa aacttattta
     3241 ttattgcttt attagtttca aaacgagtac tcccccctag tctagtagat ttagtattgt
     3301 ttactaaagt ggtactcatt gaagctgttc ctcctattac ataagacttt aaaccactag
     3361 ttgaaaaagg tatactttgt ccattagtaa gaattattgg tgctttatct cttgctgcta
     3421 cagatgcaat acttatagca tctgcttctc ctttatatgc attggttagc ataacagtat
     3481 tgactttttt tattgaattt atttcttttg caacattata acttgttttt attctgtcat
     3541 ttcccttaat tcttacaact ttcattttct tagattttaa tgaattttcg acagatgtac
     3601 taatagaata tgttcctcca attatataaa cttttgatac attttttaat cttgcagatg
     3661 tctcagttgg tatagtattt ttttcagtta atagtatagg ggcatttaaa gctcctgcta
     3721 gtccacttgc acttaatcca tctgcaatag agttactagt attaattaat atagcagttt
     3781 tatatgcatt tttatctgct attttacctg cgatttcata cttatttttt ccttgtattt
     3841 tttctacaga tgatattgca tctactaatt gaatatttgc aaatacaaaa gaaatactta
     3901 aagtcaatgt taatattctt ttatagaatt tcataat
//
LOCUS       DAFKMX010000256         3934 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFKMX010000256 DAFKMX010000000
VERSION     DAFKMX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.263"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            290..967
                     /locus_tag="LZB08_003505"
     CDS             290..967
                     /locus_tag="LZB08_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316967.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1039..3054
                     /locus_tag="LZB08_003506"
     CDS             1039..3054
                     /locus_tag="LZB08_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBM7316968.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3104..3784
                     /locus_tag="LZB08_003507"
     CDS             3104..3784
                     /locus_tag="LZB08_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316969.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1561 a          390 c          661 g         1322 t
ORIGIN      
        1 ctcaaactag ccaatagaca agtttctaga aaaataataa atttatacgt gaaaatgact
       61 ttaagctcca ttctccaaca atcggagact ttaattagta tttaataatg aatcttaatg
      121 tttaatacaa tattcaagaa ttattcaaaa atacttaagt attatagtct agtttgtaaa
      181 gatattataa aataataata ccagttatat atgaaaataa aacattataa tttgtaataa
      241 ctataaaact tggaattata attaaaatac ctttgaatgg agtggtgtaa tgtataattt
      301 actggttgta gatgatgatg ttgatatttt aaacataaat caaatttatt tttctaatat
      361 aggttttaat gtatatacat cagagactgc taaagatgct atggatatag tagagagtat
      421 agttttagac tgtataattt tggatatttc attgccagat atagatggat ataaaatgtg
      481 caatgatata aaagaaaagg ttaacatacc tataatattt ttatctaatt atgagcatga
      541 agaagaaaaa gttcggggat ttttagctgg gggggatgat tatataacaa aaccttatag
      601 tttaaaagaa cttgaactta gaatatatgc tcgtatgagg cagtacaaaa atattacatc
      661 agttcctaat gtgttaaagt tttcagcgtt agtaataaat gtaaattcaa gaaaagtaac
      721 ttttgatgga gaaagtatag atttaactac tatggaattt gaaatattat tgtttctagc
      781 tgaaaacaaa gaacaagtat tttcgaagat agagatatat aaccaagttt ggaaaatgcc
      841 tgatattgga gatcaacata cagtgcaggt tcatattgca cagatgagaa aaaaaattaa
      901 ttctttaagc agggagcatc aatacattca aaccgtatgg ggaaaaggat ataagtttgt
      961 accataagtg agatactgta attaataaat agttcttgtt ttatttaata ctactatgaa
     1021 agagatgtgg ttgatacaat gaaaaagtat aatatactaa aaggaactct cattgtattg
     1081 atagtctctc tgttggcaat aacaggatta aatcttctgt ataaatatga taataaatat
     1141 acatataagt ctattagagc aagtaatgga attttaacta tagataatga tactttcact
     1201 aaaggtaaac ttgtcttttt gatagatgac tgggagtttt ataatcataa aatatttaaa
     1261 ccgaatgatt ttacagagca agatataaaa ccagaatatg tttttatagg tcaatatcca
     1321 gatttttcta tgagaaaggg gaatcagtca ccatatggga atgctactta tcgtatgaga
     1381 ataaaaaatg aaggcaagga taagctacta agtttagagc ttcctgagat tttttccgca
     1441 agtgaagtat ggataaatgg agaaatggtt tctaaattag gagatgtagg aacagaaaaa
     1501 tatagaccca aaattaaaaa ttcagtagta agttttattg caaagaaaaa tacagaaatt
     1561 ataatcaatg tatcgaattt ttcacattat tatagtggtt tgtattatcc gcctgcatta
     1621 ggagaaacaa aggacataag taatatgatg ttttatcgat tactttttta tagtataatt
     1681 tgcttcacaa cttttgcaat agctatattt tcattgtctg tatggtttct ttcaaacaaa
     1741 agtaaaattt acttatattt tggatgtatg tgtgttttct ttgccatcca tgtatcctat
     1801 ccctttgtac atttgttagg actgcctttg gtaagcttta catatgcggt tgaagatttg
     1861 tcatatttta tggtagtttt atgtattatt tgtataaatg gaagtatatc caaaatagaa
     1921 gaaaataaag tgtatagatt tttaatttta ccattaggaa ttacaatgag tatcatatct
     1981 atagtgatac caattattct tcttccatat gatatgtact ttgtcaacat atatgggaag
     2041 ttaatagaca tatataaata tattgtattt atatatatat taatgtcttc gctggtttca
     2101 atatataaaa agacatgctc tgagcatata agttcttata ttttagtttc agtaaacact
     2161 gtttttggaa ttagcatatt atttgatgca gttacgagta atagatttga gcctatattt
     2221 acagggtggc aaacagaata ttgtgggttt gtaattgtta ttttattttc tgttatgatg
     2281 ataaacagga atagaataat aataaaagag aatgaaaaac ttacaaggaa tcttgaacat
     2341 gaagttgaag tgagaactaa tgaacttaca acattgttaa atgaacgtaa aagattcttg
     2401 gcagatgtag cacatgattt aaaggcacct gtttcggcta tacaagcatt tatagatttg
     2461 ataaaggttg gtaatattca tgtagatgaa gagacaagaa cctatcttta tgctataaat
     2521 caaaagtcaa atgaagtaca aaatagagta cgtagtttgc aagaatttac atcacaagat
     2581 aaaagcattg gtattagtga aaaaatatgt ctgaataatt ttattaaaga gatatatgaa
     2641 aataatttgc cagatacaca agcatgtggt gtaaactttg aagcttacat attggagaaa
     2701 aaaatatatg caaagataaa taaagagatg cttacaagag catttgaaaa tttaatttat
     2761 aatgctttga cctttactcc atttgatgga aagataattt tatcaatgga taaagaagat
     2821 ggatttgcaa ttattaaaat ttctgataat ggaaaaggaa tacctccaga aaatatacca
     2881 aaggtatttg acagattctt tacagaaaga gataataatg agccaaaagg gcaagggtta
     2941 gggctatata ttgtcaaatc cattataagg gagcatggtg gagaaatctt tgtagattca
     3001 gaactaggaa aaggaactgt ttttacaata aaattaagtc tatgtttttt ataataatag
     3061 cttatttaat ataaaagaat aatgatattt gggagtgatt ataatgcaaa ctaagatatt
     3121 gataatagat ggagacaagg ataattgcca gaagctaaaa ggatttctgg aggaaaaagg
     3181 aataagtata gatttagcat ataattgtga agaagctatt ggaaaaatat ttagcaataa
     3241 atatgattta atatttttag aaataattct aagtgatggt gatggatgga ctctttgtaa
     3301 aaaaattaga aatgtaacta cttgtccaat tgtttatatg acttatataa atgaagacca
     3361 aagcatttta aatgctttaa attctggtgg agacgattac ttaataaagc ctctaaattt
     3421 agaaatatta tatgcaaaag taaaagctat tttaagaaga atgaactcat atgtaaataa
     3481 taatgaaaat aatacaaaat tagaggaata taatagaact agtggagtaa taaagttaga
     3541 agataaaata ataaaattga ctccaacaga aaataagttg ttaaactttt ttatagagaa
     3601 tcctgaaaag actttgacta ctaaagagat atatgaacat gtatggatga atgaatactt
     3661 agaggataac tatagtgtgg tagttgcagt gaatggatta agaaagaaaa tagagaaaga
     3721 ttataaaaat cctcagaaaa ttataacaat aagaggagca ggatattatt ttaataaaga
     3781 ataataaata tattaatatc attaataatt gttataaatg tgaaatacta acaatatcta
     3841 aactaaatta ttataaaata gctgactgta atttaattga taaagcctat tcttctagac
     3901 tttgccagtt aaattacagt cagttttatt atat
//
LOCUS       DAFKMX010000257         3908 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFKMX010000257 DAFKMX010000000
VERSION     DAFKMX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.264"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            162..1874
                     /gene="ptsP"
                     /locus_tag="LZB08_003508"
     CDS             162..1874
                     /gene="ptsP"
                     /locus_tag="LZB08_003508"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBM7316970.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            2197..3747
                     /locus_tag="LZB08_003509"
     CDS             2197..3747
                     /locus_tag="LZB08_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7316971.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            3773..>3908
                     /locus_tag="LZB08_003510"
     CDS             3773..>3908
                     /locus_tag="LZB08_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316972.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     L"
BASE COUNT         1513 a          366 c          735 g         1294 t
ORIGIN      
        1 ataagatatt tttaatcata aattcagagt aaaaatacag taacaaatag tttataaaat
       61 tttataaaaa attataagta aataaaacta taaaaatgta aattatttat ataaatttat
      121 aaagtttatg tgctagatga tattttttag gaggtagggt tatggcttac aaaggaatag
      181 gtgcttctcc tggtgttgct ttagggaaag cgttagtagt tgaacatagt gaattagtaa
      241 ttgaaaaaaa atctatagat aatgttgaag ctgaaattgc taaattagaa gatgcagttg
      301 cagtttctaa agaagaatta gtaaaagtta aggaaaaggc ttctgaagaa ttaggagcag
      361 aggaagcaga aatatttgaa gcacatttat tagttttaga agacccagag ttaattggtt
      421 ctgcaataga taaaattaaa actgaaagtg taaatgcaga gtatgcttta aatgaaataa
      481 aagagatgtt tgttagcatg tttgagtcta tggacaatga gtacatgaaa gaaagagcgg
      541 cagatataaa agatgttaca aatagaatac ttagacatat attaggaatt aaagttgttg
      601 atttatctgc tttaagcgaa gaagttgtat taatagcaca tgatttaact ccatcagaca
      661 cagcaactat gaataaaaaa atggttttag gatttttaac tgatataggt ggaagaactt
      721 ctcatactgc tatcatgtct agaactttag aaatagctgc aatcgttgga ttaaatgata
      781 taacttcaaa agttaaggat ggagattttg tggttttcaa tggagacaca ggtgaagtaa
      841 tcgtgaatcc tgatgaagag actataaata aatatactga attaaaagct aagtatgaag
      901 atgagagaaa agcattacag ctattaaaag gaaaaccatc tgtaacttta gatggaaaac
      961 atgtggaatt agctggtaac ataggaacac ctaacgatat agaaggtcta ataaagaatg
     1021 atgctgaagg tgttggtctt tatagaacag aatttttata catggataga gattctttcc
     1081 caacagaaga aatacaatat gaagcataca aagctgtatt agaaggaatg gatggaaagc
     1141 ctatagttat aagaacattg gatataggtg gagataaaga attaagttat ttaagtatgg
     1201 aacctgaaat gaatccattc ttaggttaca gagcaataag actttgccta gatagaaaag
     1261 atatatttaa gactcaattg agagctttat atagagctag tgttcatgga agattaagaa
     1321 tcatgttccc aatgatttca tctttagaag agttgttaca agctaaagaa gttgtaaaag
     1381 aagttttagc agaacttgat tctgaaggtg tggcttatgc taaagatgta gaaattggta
     1441 tgatgataga agttccatct gctgctgtga tatctgatgt attggctaaa cacgtagatt
     1501 tcttctctat aggaactaat gatttaattc aatacacttg tgcagttgat agaatgaatc
     1561 aaaagataag ttatctttac aatcaattta atccagctgt tttaagactt ataaagactg
     1621 ttatagacaa tgcacataaa gaaggaaaat gggcaggtat gtgtggagaa tctgctggag
     1681 accaaaaaat gataccaata cttttaggta tgggattaga cgagttctct atgtctccaa
     1741 tatcaatact tcctgctaga aagttaataa cttcagttaa agagtcagat atgaaaaaat
     1801 tagctgatga tgtacttaat atgggtactg ctgaagaaat aaaaagctat atagaaaaga
     1861 cttttaatat ataattaatg ttatatagtt actaaaattc atttttctaa ataacaaaat
     1921 tatattttac gaataaaaaa actatagtat atcatatata ctatagtttt tttgttatat
     1981 aaattacgta tcaaaatttg taaaatgaat agatataatt actaaatatg tgatagtatt
     2041 ggctataata ggatataaaa aataatattg taataaaaag tttatattta gtaatgaaaa
     2101 tttaacgttt tacttgaaat tatgtgtttt gtaatgtatt attaaggttc taataggaac
     2161 attttcatca atttttctat agtgggggta ggaattatga ggtacaaaaa aagaaatata
     2221 gataagtcaa gtgaactttc atttcaatct ctagcaaaat attttataac tttctatata
     2281 gtaattatat taattattct ttctatgttt tgtataaata caatatgggt tatgaaaaaa
     2341 gataatcaag atttattatc tacaacgtca aatcgtattg aagatagggt aggagaaaca
     2401 attaagctat tgaactcttt gagtaaagat aattacataa tagataatag tgtgaatggc
     2461 atagataaaa gtaaagaact ttcaatgtat agtgatgaat ttgggtatat gatgatatgt
     2521 tttgttgata agaatataat tgtatgggat gaagaaggtg gtaatattag tctagctggt
     2581 cgtgattata tgcagaaact ttttgcaaca aaaaaatatc aagttacaga tagttttgca
     2641 gcaggggctg atggaaaaac tttaaactac actattgcat cacctataat aaagaatgga
     2701 aatgtagatg gttgtgtatt tgcagcaatt tactttgatg agataaagga tatactcaaa
     2761 caaggaaaag gtcaacagca acagaatttc atattatttg gaagtgaaaa tcaaataatg
     2821 aatttaacaa gtgatgaaac tgcatatgga gagaagtata tgaagttatc gcagaaaaca
     2881 aaatatcttg gaaccaatgc aaaaaaagta gaaaaggata tgtttaataa taaatcaggt
     2941 gattattggg cttttgaaaa tggaaaatta tactttatgt cctataaacc aattaagaac
     3001 acaaattgga gtttattttg taaagttaat ttcttagata tttttgcatt aaatttaatt
     3061 cctatgggag ctattctatc tgttataact ttagtattta ttattttgat ttgtatggag
     3121 aaaagcttca tgaaaaaacg aatggaagtt attgatatgc ttttaggttc tgttcaagaa
     3181 ttagaaaaac gtatatatca aacagaaaga cctagtgaag tagactttaa agaaattatt
     3241 cagctcacaa gtaaaggtct gactgatgga ttaactggtg ttgtaacacg tactgtattt
     3301 tttgaccaaa tttataatcg tgttgaaatg gaggaaaatg ataaattatc tgtaatttca
     3361 tttgttgatt tagatgattt gaaatgtatt aacgacttat atggacatga aattggggat
     3421 atggttttga aaaatgttgg atatgtactt cgtgaatatg agataaaata taatggttta
     3481 attgggcgct atggtggaga tgaatttgtt atgttgttat ctggttttac atcgagagaa
     3541 gaggtggagg atttattgga tgagattgct attcgcttac atactgaagt actaacagat
     3601 gatggtatca ttcaaactca ttgtagtgtt ggagctgtag tatgtagtgt aaaagacaat
     3661 aaagatatag ataatttatt aaaacaggct gatgaagctt tatactatgt taaacgtaat
     3721 ggaaaaggta attatcattt agtttaatgt atgtggaagg agtttatatc atatgaaaaa
     3781 tgtatctaaa aaattttttg tatgtactat tattgtatta ataacatttt tatgttttag
     3841 taaatttgct atttatgttt ttgacaaaca agaaaagaaa gaaacaaaag tagtagaaaa
     3901 catgttaa
//
LOCUS       DAFKMX010000258         3880 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFKMX010000258 DAFKMX010000000
VERSION     DAFKMX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.265"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(146..529)
                     /locus_tag="LZB08_003511"
                     /pseudo
     CDS             complement(146..529)
                     /locus_tag="LZB08_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      755..852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            907..1083
                     /locus_tag="LZB08_003512"
     CDS             907..1083
                     /locus_tag="LZB08_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316973.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1309..1509
                     /locus_tag="LZB08_003513"
     CDS             1309..1509
                     /locus_tag="LZB08_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBM7316974.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2381..3226
                     /locus_tag="LZB08_003514"
     CDS             2381..3226
                     /locus_tag="LZB08_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7316975.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3450..3614)
                     /locus_tag="LZB08_003515"
     CDS             complement(3450..3614)
                     /locus_tag="LZB08_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316976.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1471 a          478 c          447 g         1484 t
ORIGIN      
        1 tataacattt atttttttaa cttttgtcag taaaatgtca gcaaaacact ttatccacaa
       61 tttttatata agttttccac aacttatcac caatattatc cacataaaaa aggtagcact
      121 tatattatta gctacctttt atttattaat ctttatattt ccagcttttt atccaacttc
      181 ctatttctat tattttttct tcagttattt cttctccatc atgtaatttt gaaattaatt
      241 ctgataaagg attgttatgt aaacctctaa aaactctttt tccctctatt ttgagatatt
      301 ctctctcttt tattaatact gtatagtgta catgcctccc aattctctat gatattaaaa
      361 aacctttttt ggttaatcta gctaaaagta taattgtagt tgtatgcttc caaccatatg
      421 ttttttctat ttcatctgca acatcttttg atattacagt tttataacca gtattccata
      481 tgcatttcat aatttttaat tctgtatctg gtaatttttt atttagcata atatattcct
      541 tctttcaatt tatgtcactt cttcaaattt atctagctta aattataata ggagcttttt
      601 agaacaaaaa ctatatttaa tttttaccag gtactctacg tgtttagtct gtttttaagt
      661 gtaaaataat gttcatatct ataaatttat ttaactttca aacaatcctg ttgaataata
      721 actaattcta ttgtattatt ttagttgtag aataaaacta aattggcaaa actagagaaa
      781 tttagtgacg caaagctata gggactaaga cttatataaa tatcttatga gttatgtcag
      841 ccagttgcca aagagatagt gttctttttg tttttatgaa agttttttag ggggataata
      901 atttatatgt ttggaaataa aatggataaa tgtacacatg tattgactgc ttatattagc
      961 agttcgtatg attattgtaa ttttttagat acacagttag atgattttat attagagtac
     1021 ggagaaaatg tagtagagtc ttgtttacac caagtaatgg tattggtaag taagtataat
     1081 tagatagaca ttgatttcta gaaaaccctt tttagtttta tctacattta tattaatata
     1141 tagcaatgta aaacgtttaa atatattttt aatattaaga ataattaaaa tgttaactaa
     1201 aaaatataat taagtagaca tttctattaa tctatgatac aatagtattt gtggtaatac
     1261 acaattaaat caagtatatc ttgatatagg ttaataggag tatttttaat gaataatgga
     1321 atagtgaaat ggtttaataa tgaaaaagga ttcggtttta tatcaatgga aggtagagat
     1381 gatgtgtttg ctcatttctc agctatacaa acttcaggat ttaaatcatt agaagaaggt
     1441 caacaagtaa gctttgatat agttaaaggt gctagaggtc ctcaagcaga aaatataact
     1501 atattataat atatagattt ttcgggaaag actcttttta gagtcttttt ttattgttaa
     1561 tatatgtaag ataatcattg gtgaagtaaa taaggtatat tgagtaatgt tcttgatatt
     1621 ttatatgcta atgaatttca aaaaaataag cactcctata aaaagagtgc tcttaatgta
     1681 aatataaacc ttcattggtc cagcctaata ccccgtattc tccaagttct aactattttt
     1741 tatatacatt atcactttca tccatatcta acacaattat ttcttcttta gaatcaattt
     1801 gtaaaatatg aggactgtgt gttgtgacaa ttatttgtgt ttttaggaga attgttttta
     1861 atgtaataaa aattttacat accacatagt tgatataaaa ccccaaaata aacttagcat
     1921 ctccaatgtt tacacatata caatgctctt aaaattgcag tgaacgcgca atagtgcaat
     1981 tgataatact tattatatat tctcaatgcc ttatatttca actgctaaac ctcaatttgt
     2041 cacaaatatc gctcactgca aaatttctat atttttatta tatcataaca tataattaat
     2101 attttcaatt aatttttaca tttaattatg tttaaattct ataataatgc aactttaatg
     2161 tttctttctg gaactaatat aaaaactatt tcagaaagat taggtcatgc agatataaaa
     2221 ataactatga atagctactc tcagttttta gaagagatgg acagagaagc atttgaaaat
     2281 ttgagcaaaa tactatttat tatattttaa agaagatgac ttattcaata tttgatgcta
     2341 gaatataatt aataaaaatt taattatagg tgattgtatt atgaatatta ttaaaagcat
     2401 aactttactc ttaacattat taattgtgat attaatgaca ggctgttccc caagagaaag
     2461 tgctagtgaa gttgcaaaaa aatatattga taagatgaac tctggtgact atgaaacagc
     2521 atatagttta ttaagtaaag atagtaaaaa aaaaattagt atagaaaatc ttgaagaatt
     2581 tcaaaatatt ttagttagta caaaaagaat aattagttat aaaataggta aagaaaaaat
     2641 cataaaaaaa tataaacatg atggaaaaga atataaaaat gttgttaaat tagaagaaac
     2701 ttttaatata aaaaaccttc tttataaaaa agatgattct ctaaaagtaa atagatattt
     2761 tgtagttgaa aataacaaat acaagctatt attgtatgaa agtttcaaaa aagaatttgg
     2821 tattaatagt atggaccttg ctcaacttaa gcttaatgat tttggactaa aatactttaa
     2881 agaagttgac cttatactaa aaaaagctat tgctattaat cctactgatt caaagcttta
     2941 ttatgcccaa gcatgtaatt atatgaattt tgatggattt cctggttatt cagatacaac
     3001 atataatgct ttagattcaa ttaataaatc tttaaagtat ttagataaaa atgattctga
     3061 ctacaaaaaa aatgcaagta atatatataa cttaaaagga gttattctta tgtttcacaa
     3121 aagatttgat gaagcacaac aatatttaaa taaagcctta tactttaaca aagataatga
     3181 agacccaaaa tataatctaa atacaataaa aaaacatcta aactagatat ttaatttttg
     3241 aatttataaa atattttttt atgtataatt cagcactgaa tcataatctc tactaaacta
     3301 attacaagtt taaattataa agacttattt ctatataaat attactctat aacaataagt
     3361 ttcttattta aattttaaaa tattatctaa aaatcataaa aaagaaggta taaagagcta
     3421 ccctcaagct caatatacct tataaatatc taaacttcat tttttactaa tttttctata
     3481 tctttgataa tatgttcttt agattttttt ccagctatgg catcaaaaat tgcaacagcc
     3541 tttttctctc caaacttttc tgctacatca ttaaagtcta aaaatctaac actttctttt
     3601 ggtttaccaa acatataaca cctctcatac atttttatat atttattata acttctatag
     3661 acttattttt caccaataaa cgttagacaa attagtccct ttattttttt aaactagctt
     3721 taccttcaaa attcttaaca aaaacagttt attctactct ataaataaaa atagacaatt
     3781 taatttgtag gaaactgata atagaataca gtaaataaaa ctttgtttaa tctatctgaa
     3841 aaagttatct attaaaactg ctatgaattt ataaagaata
//
LOCUS       DAFKMX010000259         3859 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFKMX010000259 DAFKMX010000000
VERSION     DAFKMX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.266"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            784..1536
                     /locus_tag="LZB08_003516"
     CDS             784..1536
                     /locus_tag="LZB08_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316977.1"
                     /translation="MLKVNQLYKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1526..3637
                     /locus_tag="LZB08_003517"
     CDS             1526..3637
                     /locus_tag="LZB08_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316978.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVINKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDNYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMNEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANKDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLSNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1456 a          540 c          500 g         1363 t
ORIGIN      
        1 actacagact tttattatta caatctctta tttattacag ttctttgcta tactacagac
       61 ctttattatt acaacatttc tattatatta caatctctta tttattacag ttctttatta
      121 tactacagac ttttattatt acaacatttt attattttaa cttctttact ataatatagt
      181 aatattttaa tatctcaaaa tcatatcaat cgcttttcag catagtctaa aatacttcca
      241 ctttctaaaa ttatctatca tacctatact aacaatactt tttaaccttt ttattataca
      301 tttcaattgt ttttaacaac ctatttattt ccaaaactcc aatatatttt ctcaattaca
      361 ttgtgttaat aacccctaaa tcctttcaaa ttatttacaa tacattccat taaataatct
      421 tactgctcta taattctttt tttcttttta accctactat acattattta catcattttt
      481 ttcaatacaa cattcgttat tctataacct ttttactttt actatatacc ttttttctaa
      541 ctacaccttc aatgttccac atcatattac tttagcacaa caattaatac ctaaaaataa
      601 ccctctaaat cctccaataa tataatcaat ttaactataa taataacctc tcacactatc
      661 caaacaatat attttcaaac attacaaaaa aacatccaac tattacaata tagctcatat
      721 ccctcctccc ctttcaaatg ctatcattga cttacaagat aaatgaaagt gaggatgata
      781 tatatgctta aagtaaacca attatataaa tcatataaaa caggtagtaa aacatatcct
      841 gtattaaaaa atatttcttt tgaaatcaac aaaggagagt ttgtcgcaat catgggttca
      901 tcaggaagtg gaaaaactac tcttttaaat tgtatttcat gttttatccc aattgataat
      961 ggggaaataa ttcttggaaa taataatata agaaatttaa aagaaaagga actttcagaa
     1021 gttagaaacc aacaactagg atttgtattc caagatttca tgctatttga tggtcttagt
     1081 gtatttgaaa atatatgtgt tccacaaatt atcaaaggta gtcaagtctt acaaatggaa
     1141 aaccgtgcaa ttaatctatg tgagctattt ggtattgaac atatcagaaa aaaataccct
     1201 gccgaaatat ctggtggaga aaaacagcgt acagcagtag caagagcact catgaacaac
     1261 ccagatttaa tattagcaga tgaaccaact ggtaaccttg atagtaagtc ttgtcgtaca
     1321 gtcattgaat catttttaaa agcaaagcaa gaattagatg ctactatact catggttaca
     1381 cacgatagct ttgcagcaag cttctgtgac cgagttattg tgcttaaaga tggaataatt
     1441 tataaagaag ttgtaaaatc aaattcaaga cataaattcc tagatgaatt actagaagtt
     1501 ttaaaagaaa tgggaggcgc tcaagatgac aattaataaa ttatacagta aacttcgagc
     1561 ctataattgg aagaactata caactttatt cttttgtata atactttctg tggtattagt
     1621 aacttcatat gctctaattt attttagccc aacaatacaa aacatacttc caactggtgg
     1681 agatagcaga aaacaggcta caatgatatt ctcagttgct atattaggat gcgctgtctt
     1741 tactacatac gcttcttccc tctttttaaa atttaaaagc aaggaaattg gtattttcat
     1801 ggctctagga acagataaga agaaattaaa aaaacttcta ttttcagaga tattgttaat
     1861 ttcaatagta tgctgttttt taggtcttat attaagtatt cctgtatctt ttggtatatg
     1921 gaaaatattt caattagtga ttattgatac cagagaaatg acatatcatt ttggaactca
     1981 aggattcata tatggattta tatttagtgt atttgtgaca ttatgcatat ttatactttc
     2041 tgtaaaattt attaatcgta caaatataat tgatatacta aatgaccaga gaatatctga
     2101 accaatacag gatgtaaagg actggtatgg tatattaggt ataattttta ttatactcgg
     2161 tatttttcta ggatatctcg taccttcact aactataaac aaattaaatt atagactacc
     2221 atctgtatgg agctttactt atctactctc tcttattgga atatatatga ttatgacata
     2281 catagttgta catgctaaaa ggggaaaaca tccagagaaa tactataaaa acatcatatc
     2341 aactagcatg atgagattta tgggaagaca aacagtaaaa aacatgtgtg ttatttcatt
     2401 tttgattgca ggagcattat tcgctgtatt ctatgttcct aatatggtta caggtatcta
     2461 tgatactctt aaaaataacc ctatagatta tacttttacc tacaatcaaa gagataaaca
     2521 ggtatcaaaa aaagatattg aacaattagc agataaacac aatataaaaa ttcaaaacta
     2581 tatggaaatt ccttctatag aattaattgt tgatggtgta gaatcaatat atcataaaga
     2641 tggaactatc gaaaaggtat atatgaaaaa aagctcctat gatatgttct ttaattctaa
     2701 agatttttcc aagctaattg gcaaaccaat aactataaaa aatggtgaat atcttgctgt
     2761 tataaataaa ggaaatgaaa atcaagatta taaatatacc aaatttatat caagcccagt
     2821 cacagaaaaa gaaatcccct ttagatatgc tggaaaaata atatttaatg gttctttttt
     2881 tcaaaataga agtatgaacg cctatgtcct taatgatgag gattataaat catttactaa
     2941 agatttatcc ataaaaaatt acaataattc tatactattt aatgtagata aagataacta
     3001 tgcttttgcc aatgacttaa agaaagaaat tataaaaaga acatctaagg aaatgggcat
     3061 gttcagtggt tatgatgaat acgtaaaaaa acgttatgaa tcaactggaa gaaaatattt
     3121 tatgaatgaa gaatatccaa gtggtgaagg tcatttaaaa ctagacccaa acaacaatca
     3181 gttgtatctt aattggaaat actatcctaa ctttaaaatt ttacaaagcc aagatatgat
     3241 aaaaaacatg gcagtcttct taatgttatt catatatgtg gcaatcatat gttttacagc
     3301 agtaggaatc attgcatata caaggggtgt tactatagct ataaatcaca agcaggtctt
     3361 tatggattta aatcgattag gagcaaacaa ggattatata aagttctgta ttagaaatca
     3421 attaaagaaa gtattttcat accctcttat tgcaggaagt tttttaattt atctgtttaa
     3481 ttttatgatt atgttaagta atgatggtag aatcgtcaat agtgaggtca atgctctttt
     3541 gataaactta ggttttatag ggatttgtgg ggtttatatg taccttatat atctattaac
     3601 ttacaagaaa ttcaaaaaaa ttattggtat tttataataa ggaaggttgt tagattttta
     3661 aagtaataaa atcattggtg ttttataata aaaaaggtta ttagattttt aaagtaataa
     3721 aatcattggt gttttataat aagaaaggtt attagatttt taaagtaata aaatcattac
     3781 tttttataat aataaaagca tttgtatttt ataataagaa agattgttag attttttaat
     3841 aagaaaatca ttagcactt
//
LOCUS       DAFKMX010000260         3856 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFKMX010000260 DAFKMX010000000
VERSION     DAFKMX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.267"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            65..1177
                     /locus_tag="LZB08_003518"
     CDS             65..1177
                     /locus_tag="LZB08_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBM7316979.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1385..1861)
                     /locus_tag="LZB08_003519"
     CDS             complement(1385..1861)
                     /locus_tag="LZB08_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316980.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2060..3685)
                     /locus_tag="LZB08_003520"
     CDS             complement(2060..3685)
                     /locus_tag="LZB08_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7316981.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1351 a          613 c          423 g         1469 t
ORIGIN      
        1 aaaattattt tgtgatatat atatgttata ataaatgtat aaaattttga ttggtgggtt
       61 atatatgaat gcatttatta gaaaaagaaa taaaaactat gttgtttacc tggaatttga
      121 agatgaaaag acaggtaaaa gaaaacaaaa aaatatgggt acttttgata aaaaaaggga
      181 agcaagtaaa agactaaatg aggtaaaaga aagcttatat aatgatggtt ttttagtacc
      241 aagcgaaatt acagtgtcag aatttctatt agattttcta aataaatata gtgaaaatat
      301 atctttagct acatataata attatgttag tatttgtaag aattacatta atccatctat
      361 tggaaaatat aaaatacaag acttacatcc ggttcacata caaaactaca tagataaact
      421 atcttataaa ctaaatcctc aatcaattaa gattcatata aatatattga aacttgcaat
      481 aaaaagagct tatagactca aattaataaa agaaaatgtt atggatagta tagaggctcc
      541 aagatataaa aaatttaaaa atgagattta tgatagagaa caaatgataa aattattaga
      601 tttagcaaaa aatacagaaa tggaattacc tattaatttg gctattggat taggtcttag
      661 aatttcagaa gttttaggtc taacttggga taatattgat tttgaagaaa atacaataac
      721 agtaaataag ataacttccc gaattaatgg ttctgttatt ctcaaagagc caaagacaga
      781 aagctctgtt agaaaaatat cttctcccaa agaattaatg tctttattaa aagaatataa
      841 aataaaacag aataaaaatt tattaaaatc ttccattaga aataataata atttgttgtt
      901 tttcaataag aaatgtgaac ctattgctga agatgttatg agtaaaaaat ttaaaagatt
      961 tttagaaaag aatgaattac ctcatattag attccatgat ttaagacact cacatgttac
     1021 tttacttata aattctaaag tacctataaa ggttatatcc gagagagtag gacactcaaa
     1081 tataagtact actctaagcg tttattctca tgtacttaaa gaaatggata aagaggcttc
     1141 tgacaaaatt tctgaaagtt tatttaatgc taattaatat taatttatct tagctttaaa
     1201 ttagcttagc ttattgagat agattcatta atattaaatg tatctatctc aaattaataa
     1261 ctacttaaat aatctgtttt attttaattt atcacctcag ttattatact tttttacgaa
     1321 aaattatact ttacaataaa aaagatattc ccttatagaa aatatctttt taaatactat
     1381 attattatat acttgctaac attggaatat ctaacatctt cttcattaca tcatctactt
     1441 tttttgcttt atattcattg tatccaatta aatatccttt ttcatatgaa ctttttaaaa
     1501 tctctttttc atataaatta tcagatttgg cttctgtgac acttaaatta taaagccctt
     1561 tacacccatc ttcataacct ttattatacc ctttttctgc aaaatagttt ttatgatttt
     1621 tttcgttatg tactgaagat attgttactc cagaattttc ttttgtacta gcataaggac
     1681 aaaaaccttt attgtgttga tgtgctgaat gtcctccaca ataataacgt gttaactctg
     1741 atttattttt attatgatga cctcctgatg aattatgccc tcctgagtga gcatatgaaa
     1801 aattccctac actaatcata gctaaactaa ttactacagc tactaacctt ttcttaacca
     1861 taatctattc cccccaaaat gttatatatt tttattatat catctactat attatattta
     1921 aattcttaat atacaaaata ttactctatt ctgctaataa acatgtttaa acaacataaa
     1981 aaacctccta atatttgatt aaaaatttac acactcatgc atagcatgtc tcaaatatta
     2041 aaaggtttta tactaataat tatttatctt ttccatacat tttagctaat tggtcttgta
     2101 tctctttact ttctaaatac tcatcaaagt ccatttttct atccattatt ccatttggag
     2161 ttatctctat aattctattt gctatagtag atataaattc atggtcatga gaagtaaata
     2221 aaagcactcc tgggaacttt tcaagtcctt tatttacaga agttatactt tctaggtcta
     2281 agtggttggt agggtcatct aatacaagta catttgcatt tgaaagcatt agcttactaa
     2341 gcatacatct aaccttttct cctccagata taacttgagc ttcttttaat gcttcttcac
     2401 ctgagaataa cattctacct aaaaatcctc ttataaaact ttcacttttt tcttcagaga
     2461 actgtctaag ccagtcaacc aaagaatatt ctactccgtc aaaaaactta ttatgatttt
     2521 ttggaagata atcttgagaa gtagttactc cccatttata ctctccactg tctggttcaa
     2581 gttctcccat aactatgttt aaaagtgcag tagtagctat ttcatctccc ataaatacta
     2641 ctttatcatc tctatccaat ctaaatgaca cattatcaag aaccttaacc ccatctatag
     2701 ttttagttaa attgtgtact tctaaaactt cattcccaat ttctcttgca ggtgtaaatc
     2761 ctacatatgg atatcttctt cttgatggtt gtatatcttc aacttctaat ttctctaact
     2821 gtttctttct tgatgttgct tgtttagcct ttgaagcatt agaactaaat ctagctataa
     2881 attcctttaa ttgagctatc ttttcttcag tctttttatt ttggtcctta gccaattgta
     2941 aagccaattg acttgactca taccagaaat catagtttcc aacgtacatt tgtattttac
     3001 caaaatctac gtcaactata ttagtacata tttgatttag gaaatgtcta tcatgtgata
     3061 ctattataac tatagattct tctaaatcca ttataaaatt atttaaccag tttattgatt
     3121 taaaatctaa gtggtttgta ggttcgtcca ttaatagtat ttcaggctta ccaaaaagtg
     3181 attgtgctaa caatacctta accttttccc caccaactag ttccttcatt tgtttgtaat
     3241 gcatatcttt cgttatgcca agacccataa gtattttctc agcattagtt tctgcatccc
     3301 atccgtcaag ctctgcaaac tcaccttcaa gttcggcagc ttttatccca tcttcttcat
     3361 taaagtcttc cttcatatac aatgcatctt tttctttcat tatgttccaa agtctctcat
     3421 gacctcttat aacaacattt aaaacagttt cttcttcata ttcaaagtgg tcctgtttta
     3481 aaactgacat tctttcttta tcagttatag atatactacc tgtattaggc tctatttctc
     3541 ctgataatat ttttaaaaaa gtagattttc ccgcaccatt agctcctatt attccatagc
     3601 aattaccttt agtgaatttt aagttaacat ctttaaatag ttccttatca ccaaatctaa
     3661 gtccaacacc tgtaacttgt aacattaata tattttcctt tctttttgct cataatttat
     3721 tttatcataa aactttgata acatttttat tatatattat cacgtaaata gtatattttt
     3781 tatactaaat ttgtgaaata tttattaaaa taatactaaa aattgtaaat ttataaaaat
     3841 taacaatctc ttatat
//
LOCUS       DAFKMX010000261         3855 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFKMX010000261 DAFKMX010000000
VERSION     DAFKMX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.268"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            3..863
                     /locus_tag="LZB08_003521"
     CDS             3..863
                     /locus_tag="LZB08_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316982.1"
                     /translation="MAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTTFMRI
                     LATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVLSDIP
                     LRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEPTAGL
                     DPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEALAKL
                     AEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPTIEDG
                     YMLIMGGDNI"
     gene            860..2149
                     /locus_tag="LZB08_003522"
     CDS             860..2149
                     /locus_tag="LZB08_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316983.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2079..2723
                     /locus_tag="LZB08_003523"
     CDS             2079..2723
                     /locus_tag="LZB08_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316984.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMTQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(2990..3454)
                     /locus_tag="LZB08_003524"
     CDS             complement(2990..3454)
                     /locus_tag="LZB08_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7316985.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFISLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPVVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1442 a          467 c          585 g         1361 t
ORIGIN      
        1 aaatagcagt atccaatctt agtaaaagat ttgggaaaaa acaagctctt gataatataa
       61 atcttgaaat cgacagtggt atgtttggtc ttttaggaag aaatggagca ggaaaaacca
      121 catttatgag aattttagcc actttattat caaaaacaga aggttcaata catatgtgtg
      181 gagttcctat tgagaatact aaggaaatta gaaatatggt tggttatttg ccacaagatt
      241 tttcaatgta tcctaacatg acagtatata aagcaatgga ttatttagca gttttatcgg
      301 atataccact aagaaaaaga agaaaaatta tagcaaagtt attaagcaaa gtaaacctta
      361 ctaattatca aaatgtgaaa gtaaaatctt tatctggagg tatgaataga aggcttggga
      421 tagcacaagc attaattcat aatcccagag ttttaatagt agatgagccg acagcaggtc
      481 ttgacccaga agaacgtata agatttagaa atttactatg cgaaattgca aatgatagaa
      541 tcgttatttt atctacacat attgttggag atatagaagc tacatgtgaa aacgtagcta
      601 tattgaatag tggaaaagtc atatacaatg gctcaacaga agcattagca aaactagctg
      661 aagggaaagt atatagtatt gaagtagata aaaaagacat tgaaaatata aaaagtagat
      721 ttatagttat aggaatgttg actcatggtg gaaaggctat acttaggatt atatcagatg
      781 ataaaccttt tgagacagca gtaaattgta atccaactat tgaagatggc tatatgctta
      841 taatgggagg agataatata tgaatctatt tataaaagaa tgtaaaaata tattaaagag
      901 tatggtatat tatattttta ttgtagtttt agtttttttc tatatttctc aacttggaag
      961 ttcagtttca aatgatgtaa gtaaaaatat tcctaatgat ggaaatccat tagttgctcc
     1021 acaaaaagta gagaatattg aggaatatta ctctgtacac aatcaatatc catatggtca
     1081 aaaagaatct atgaaaacag aaaaggtcat tccaaatgca actataaacc taattagaga
     1141 atatcaaaaa aacacatata caacgtatcc aacaggtgta gttaagtata taaaattaga
     1201 ccaaaataaa acgaagacca tagaaaaata tatagaagaa ataactggta aaagtattag
     1261 cgaaataaat tcactttgga aaactgatga aagtataaaa atagattatt ctaaagtatc
     1321 tcttgaaaga tttgaagaaa taatgcaaag agtagacgac attttaggag gagggtctgc
     1381 atatgctttt gacaagttac aaagatttgg agtagttcca gttacctatg atgaagctat
     1441 gaagaattac aactatacaa tacaaaaaga taaagttagt aatgggtatg caagaatttt
     1501 ttgcgattat ataggtatag ttttaagtat atttccagta tttgtggcag tttttatgtc
     1561 acttaaagat aagcgttcaa gaatgagaga gattatttat tcaaaacaag tatcttcatg
     1621 caaaatagtt ttatcaagat attttgcact tgtctttatg atgatattgc ctgtattaat
     1681 tttaggtatg aaagatttac ttcctttgat tttctttgct aaagataaag gtatatctat
     1741 tgataactta gcatttataa aatatacatt atggtggata ctaccaactc ttatgatagt
     1801 tacagctgta ggaatgagta ttactatatt aacagataca ccagtagcaa ttattgtgca
     1861 attaatttgg tggctttaca gtataaattc tacaggactc aaaggtgatt attcaacaat
     1921 gggactaatg ataagacata atagtgtatt aaatggggat attattatac aaaacttcaa
     1981 tcaaattatg attaatagaa ttgtgataag cttgggagca ttggtgcttg ttttgttagc
     2041 tatattctta tatgagcaaa aaagaagggg gaatcttgat gtttgggcaa agttttcaaa
     2101 actgtttagt tctaataaaa gaaaacttca aattaagcac tttaactaat ttaatatgtt
     2161 caattatatt gctatgtatg attcctctaa tacaaggagt agctaattta gacagaattt
     2221 caacagctat atgtttagaa aattatgttg caataatagg gatagtaatg ttggtacccg
     2281 tattttcgct agagcaatca aaagaaattg atgagatagt atcttcaaaa gcattatctc
     2341 aagttattgt acaattaatt aggatactga tgtcaattat aggtatagtg gtattaatca
     2401 taggttttgt aaatgtttta atatatcttg gatgtaattt tccagtagtt gagtatatta
     2461 ttggaagttt cgcatcagct atgtttatag gaagtttagg aatgtttttc tcaaatttat
     2521 ttaataatac aatcataggt tatatgatat ctataggata tttaatttta aatatgatga
     2581 ctaaaggcaa gtatgttgga aaatttttta taatgtctat gactcaaggt agttttgatg
     2641 agaaatattg gctgatactt ggaagtgttg tgttgataat tgcatctctc ttaataaaac
     2701 caataaaaag gaagattaga taataaaatg agagcttaga taaaataaag ttaatacaaa
     2761 taaaaagtac atttatgaaa tataaatcta tttttatata cacaaaccta cttacaatct
     2821 ctttatatat gtagatagat ttatatatcc gtagtataaa atatatatgt atacaaaatt
     2881 ttatcaaaag ctctcaaatt tatatttcaa agacacttat tgctatggcc tacacatatg
     2941 tattttttca ttttaattgt tgtattaaca ataagactat tgttatagac tattgatacc
     3001 tcataacaaa aaattctcca tctcctaaat gcgttttctg taccttagtc tcaactattt
     3061 taaatccttg agactcataa cattttacag ctctcatgtt ccatgttctt acatttaata
     3121 caactggttt agtactatat ttatttctac attcttgtaa tcctaggcca attatttctt
     3181 taccaattcc cattccgcaa taattcggtt tcataccaat accaaagaat acagagcttc
     3241 cttcatctaa taagcttata aaacctatca attctttatt gtcatttata aaacttataa
     3301 aattttttct tttatcttct tttgctagtg aaaaattctt ctttttaatt tcctcccatg
     3361 gaggtagatt gtatattgaa tatttacctt catatttcca attagtaact tcaatagcat
     3421 gttcttcagt aaatttaact ttatttaaat tcatatgtcc tcctattaaa atatttaagg
     3481 taattaatgg tattttaaag ttttagaaaa taagtaaaac tatttatatt tatccataaa
     3541 atctttaaaa attttactaa caattcttgt accctgtact ttttattgga ttcacagaag
     3601 actataaact aattttgaat taccttctaa ttcaaaaaca tcacgatata tataatttct
     3661 tagcttgatt aaattctgtt aaatccgttt cttatagttg tataatcaac atcaatatct
     3721 ttatgtgtat tagttaattt attggcttta ggaacttatt attaatatat tgtatttact
     3781 ccaagtagta ggtaaattat aggcaaatta tcaaatgtat atctatccat gcttaaactt
     3841 tagagataca ataac
//
LOCUS       DAFKMX010000262         3779 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFKMX010000262 DAFKMX010000000
VERSION     DAFKMX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.269"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            315..650
                     /locus_tag="LZB08_003525"
     CDS             315..650
                     /locus_tag="LZB08_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7316986.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            787..2064
                     /locus_tag="LZB08_003526"
     CDS             787..2064
                     /locus_tag="LZB08_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBM7316987.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2185..3573
                     /locus_tag="LZB08_003527"
     CDS             2185..3573
                     /locus_tag="LZB08_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBM7316988.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1575 a          346 c          637 g         1221 t
ORIGIN      
        1 ttaacaggat aagcagttta aataaagaat tattatatta gagtgatttt atatataatt
       61 taagataata aaattttaat aaaatgagtt aaagattgta tattaataga gttgatgtag
      121 ttacaaaaaa gttaccaatt gttatgtatt tacaaaactg atatattaat tacataaaat
      181 ctaaaatagt ttgtaatagg agtatatata ataaaaattt taattaaata aagctaggta
      241 tatttataat attaataaat atattgctat aaaagtttgt ttaataaatt aaataaagaa
      301 aagaggagaa taaaatgtac aacatagatg atatgatata taattgtcca gtagaagctt
      361 tatcaaatat tttaggtaaa aaatgggtgg ctataattat atggaaaata caagaagata
      421 aaaaacgatt tggagagcta caaagagcta taccagactg tagtaagaaa atgttagttc
      481 aacaactaga ttttttaatt gaacagggga ttatagtcaa ccaaaaaaag ttggttaata
      541 atgttgtaga gtcaacatat tttttaagtg aatcaggatt acgattgctt cctgtaatgg
      601 aaaaaatgat tatgtggagc aataaaaatt taaattgtga taaatactaa aatttactct
      661 gtaagtaatg aaaatttaat taagctatga gtagaaaaat gtattaaaat tacataattt
      721 tttagttaga ttttaattta gaaatgggat tgtaatattg gaaataaaat aaggaggaat
      781 tttaatttgc aaaatgtaaa tttattttta gtatttatag aaggtatagt atcattcttt
      841 tcaccatgta tactgccaat tttgcctatt tatttgagta tattgtcaaa tagtagtgta
      901 gaaaatttaa aagaaggaaa gacgagtttt ataggaagtt ctttatttaa aaatactata
      961 ttttttgctc ttggaatctc aacaacattc tttatattgg gttcatcagt aaaagtattg
     1021 agtatgtttt ttaatgaaaa taaagattta ataatgttta ttggtgggat tataattatt
     1081 atcatgggat tattttacat gggaataatt aaatcttcaa tattgaatag agaaaaaaga
     1141 tttaatgtta agtttaaaga aatgaaagca ataacagctt ttatattagg ttttacgttt
     1201 agttttggat ggacaccttg tattggtcca atattggcat ctgtattagt catggtttca
     1261 agttcaagta atcatcttag tgccaatctt ttaatagcag tatatactat tggttttatt
     1321 ttgccattca taattacagc tatgttttac agtaaactat ttaaaactat agataagata
     1381 aaatctaaca tggaaattat taaaaaaata ggtgggatta tacttatagt atcaggaata
     1441 ttaatgatgg taaatggatt tggaagtata agtaaacact ttaatacgtc acaaaatagt
     1501 aaaattgaaa gcaagcaaga agaaaataag cgagaaaatt ctactgataa agaagaaaat
     1561 tctgatggta atgatagtca aaaagactct aataatgaca ataatgataa agggtctaat
     1621 gatgaagata gaataaagtc tattgatttt actttaacag accagtatgg taaaacacat
     1681 aaattaagtg attatgaagg caaagttgta ttcttaaatt tctgggctac atggtgtcct
     1741 ccatgtaaag aagaaatgcc ttatatagaa caactgtata aagactataa taaaaataat
     1801 gatgatgtag taattttagg agtagcttca cctaatttag gtagagaagg ttcaagggaa
     1861 catgtagtta acttcttaaa agaacaaggt tatacattcc ctgttgtact tgatgaagat
     1921 ggagcattag catatcaata tggaattaat gcttttccaa ctacatttat aattgacaag
     1981 gaaggatatg taactcagta tattccagga gctatggata aggcaactat ggcatcattt
     2041 attgaaaatc agagaaataa ataaaagtat ttaagtataa gtttataaat caatttaagg
     2101 ttacaaaaaa gtgcctaatt gtaccaccta aataatcttg ttatagtaaa agtataaaca
     2161 atacaagaaa gggttgaatt ttatatgaaa aaaacatttg atgctataat aataggattt
     2221 ggaaaaggtg gaaaaacatt agcaggggat ttggcaaaca ggggattaaa agtagcttta
     2281 atagaaaagt caaacaagat gtatggtgga acatgtgtaa atgtggcatg tataccaaca
     2341 aaaagcttag aaaattcagc aaacagcgta aaaacaaaga atataaattc ttgggatgaa
     2401 gtacaagctg aatatgagaa agctatagat aaaaaagaaa ctttaattac aaaactcaga
     2461 gaagctaact ataataaatt aaattcaaat gagaatgtaa ctatattcac aggaatggga
     2521 actttcattg atgaaaaaac agttcaagtc aaaactgaaa atgaaattta tgagttagta
     2581 gcagataata tatttattaa tactggttca agaccattta tacctaatat taagggaatt
     2641 gaaaataaga atatagtata tgatagtgag tctcttatga atctaagagt tttacctaaa
     2701 aaaatgacaa ttataggagc tggatttata ggtttagaat ttgctggtat ttatagttca
     2761 tttggagcag aagttacaat attaaactct aataatggca ttttaccaaa tgaagatgtt
     2821 gaggattcag aagaaataat aaaattactt gcaaagagaa atgtaaaaat tgttaataat
     2881 gcaaatataa aagagattaa agaagtttct gaattagcaa tagtagaata tgaagtagat
     2941 ggtaaatcta aagaattaac aagtaatatg atacttgtag ctacaggaag aaaagcaaac
     3001 actgaagggc tagggctaga aaatgcaggt atagaattaa atgaaagagg atttattaaa
     3061 gtctcagaaa ctcttaagac taataaagag catatatggg ctataggaga tataaatgga
     3121 ggtccacaat ttacttatat ttcattggat gattatcgca tagttattaa tcagttattt
     3181 ggagataaga ctagaacaac taatgataga aagaatatcc caaattctat atttataagt
     3241 ccagcatttt caagagttgg tcttaatgtt aagcaagcta aagaaaaggg atatgaagta
     3301 ttagtggcaa agatgccagt agaagcaatt ccaagagcta aacagattgg caaggcagat
     3361 gggtttataa aaatagtgat agataaaaaa tccaataaga tacttggagc tagtatgata
     3421 tgtgaaaatt caagtgagat tatccatctt attcaattag ctgtagattt agaagtagag
     3481 tatacatatt taagagaccg tgtttatgca catccaacta tgacagaagc tcttaatgat
     3541 attctatctc ctaatatgat aaaagaagta taacataagt ttaatatata aacctaaaat
     3601 tatcattata agtagatttt agaaagagac tatctcaaaa aatagagata agtctctttt
     3661 ttaatgttta aataataaaa atatatttga ttttataaaa gaagattatt ttatgagcta
     3721 aaaagtttat ttagaaacac tacctattta tgttataaag ttataaatat aatatagaa
//
LOCUS       DAFKMX010000263         3763 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFKMX010000263 DAFKMX010000000
VERSION     DAFKMX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.270"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            373..1062
                     /locus_tag="LZB08_003528"
     CDS             373..1062
                     /locus_tag="LZB08_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7316989.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1062..1979
                     /locus_tag="LZB08_003529"
     CDS             1062..1979
                     /locus_tag="LZB08_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7316990.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHIFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2052..2978
                     /locus_tag="LZB08_003530"
     CDS             2052..2978
                     /locus_tag="LZB08_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7316991.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2975..3655
                     /locus_tag="LZB08_003531"
     CDS             2975..3655
                     /locus_tag="LZB08_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7316992.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITILMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
BASE COUNT         1444 a          392 c          616 g         1311 t
ORIGIN      
        1 caaataaaag tttgttttta attatttaaa atccaaattt gattattttt aaagaaacta
       61 tgttctgtat aaaactagaa tgtggtttct tttttatgta taaaatattt tttagcaaga
      121 gttaaaaggt gttttataat ataaaatagt taattgtaga tgttaataaa agaaaattat
      181 gtatatttca gataaagata agagagtata ctatatttta attatttttt caataaagct
      241 tattgaaaag aactttaact tagaagagta tcaccaaatt gcagatacaa tatcataaac
      301 agttatttat tgacaaccct ttttaataaa tatatcattt gaagtatact gacgaaaagg
      361 agttgaataa taatggacat cttattggtt gaagatgata agaatattcg agaaatggta
      421 tacgattttc tgattagtga gaattactct gttaaaactg ccattgatgg aaatacagct
      481 attgaaatat ttaaaaatta ttcatttgat attattttac tggacttgat gttaccaaag
      541 tctagtggtt ttgaagtatt aaaatatata cgaagtgtga gtgttgttcc tgttattatt
      601 actactgcga aagatagtga cacagataaa atgctagggc ttaatcttgg tgcagatgat
      661 tatattacaa agcctttttc aatcgtagaa ttgcttgcaa gaattaaagc taatattaga
      721 cgtgcaacag tgtacagcag ttcaagaaac tctgaagata tggttattca gtataaagat
      781 ttgattataa acatatcaaa atatgtagtt tcaaagaatg gaattgactt aaacttaaca
      841 catacagaat ttgaaattgt taaattacta gcgactaatc aaggacgtgc tttttcaaag
      901 gaacaattat acaacactat ttggaaagaa ccttattatg gaaatgaaaa tgtgctaaat
      961 actcatatta acaggttaag aaataaattg tctgatggta tagaaaaaaa taattatatt
     1021 aaaactcttt ggggaattgg ctacaaaatg gaggaagaat aatggagtta tttttagttg
     1081 taatttcgat tgtactattt gctttctact taaaagaacg aaataagtca aagaaaatgg
     1141 aaagtgatta taagtatatc aattcaagac ttcgtgacat aacaaatgat gatgaaatta
     1201 attatatttt agtaccatct gatatttcga ttgtaaaaga aacagcagta gatataaata
     1261 aacttcttga aaagttttac agcaggcaaa ttaattataa tagaagtgaa aaagcgatat
     1321 tgcaaatatt tacaaacata tcacacgatt tacgaactcc catcacagta ttaaagggtt
     1381 atattgaaat gctatatctt caaagtcaaa aagaagaatt atcttcttca atgagagcaa
     1441 ctgtggaaaa aatgcaaaca aattcaaatg agcttgttaa ttcaataaat aacctgttta
     1501 atatggcaaa aatccaatct ggagatatga tattaaatct tcaaaaatcc aatctaacac
     1561 aattatgtca tgagattatt ttagagtttt atgatttgct agataaagaa aactttaatg
     1621 ttgaagttaa tattgaagat agacctatat atgcaaatgt tgacacagaa gcattacaac
     1681 gtatactcaa aaatttaatt gataatgcta taaaatatgg ttcagatggt agatttttag
     1741 ggatttcact atatgaaaag gaaaaacata tttttataga tattgaggat aaaggttcag
     1801 gtatttctga aactgaaaaa gagcatattt ttacaagagc ttatactgct gacagaaaaa
     1861 aaggcaatgg tttaggtttg gctatttctc aagggcttgt aaactctatg ggaggttcaa
     1921 tttatgtaaa tagtagacct aatgtaaaaa cagtttttac ggtaaaattg aaaagttaga
     1981 aaaaagttag atttaaaaga gagaaaagat aattttattt tgtatactgt ttttagaaag
     2041 gagggagtat gatgaactat atcatagaga cccatcaatt aaagaaaata tataaagata
     2101 aagctgttgt taatgctgtt aatattcatg tgaaaaaggg agaaatttat ggatttgttg
     2161 gaccaaatgg agcaggtaaa agtacagtaa tgaaaatgtt gctaaatctt gttaagccaa
     2221 attctggaga aatagttatg ttcagtaaga aagtggcaga aactgatttt gaaatattaa
     2281 aaaagattgg tactattatt gaaaatccgt atttatatga gaatttgact gctaaacaga
     2341 atcttgattt acactgtgaa tatatgggtt attataacaa agaacatata tcagaggtat
     2401 tggaatgtgt cggattgtca aagcaatcaa acaagaaagt ttcaaaatat tctttaggga
     2461 tgaaacaacg tcttgcaata gctagagcaa ttcttactaa accagaatta ttgattcttg
     2521 atgaacctat aaatgcactc gacccagagg ggataagaga aatgcgagaa ttattccgta
     2581 agttaaatac agattatgga ataactatat ttatttctag tcatattctt tcagaggtag
     2641 aacaaattgc ggacactatt ggaattattc aagatggtaa acttattaaa gaaatttcta
     2701 tgtcagatat acacaaatat caaacagatt atgttgaagt agatgtagat aatgtggaac
     2761 ttgctggata cttgttagaa aaagaatttg gaataaaaga ctttaaaatt acttcggaaa
     2821 gttgtattga aatatatgat ttacgaaaag atgtaaaaga aatatccaaa atatttattc
     2881 aaaatcaaat tggtataaat agcattggac gtaaacaaag ttctttagaa gattacttct
     2941 tccaaactac aggtacagga ggtaaagaaa cactatgata catttaatga aactagagct
     3001 aaaaaaagtt ggactaaaaa gatatttttt attttcaata ctggggattt tcttatgtat
     3061 gttttttgtc tttactggat tgaatgataa atctacttca atatatgatt atgatgttac
     3121 atttaggaca atcagtttat ttttctgttt ttactatatt atattatttg ctgtgttgaa
     3181 tgtggcatat ataataaatg aatatacaaa taaaacaatc ttgattttat tctcttatcc
     3241 acttgaccgt aaaaaactta ttttatcaaa gttacttttg attacgattc ttatgatatc
     3301 atcaatgata gttggatata tctgttgttt tacattcgtt gttgtgttgg ataagtattt
     3361 taatttagtt attggagatt ttcatatttc aattttatct tattggatac caggagcaat
     3421 taaaagtatt attatatttt gctcattagg tatatggaca ttcgttgttg gaatgataag
     3481 aaaatcaata cctgttaccc ttgtcagttc aattattttt atcaatttaa gacaatttat
     3541 tttagctggt acaaatacaa ctcaagacag tttgatttct gtttttatta tagttggtat
     3601 aactgtgatt ggtatatatt atatactaaa tcataaagtt actgaaattg actagatact
     3661 gtggctagga aggtagttat taattgtatc ttcttgggat aataatgatt tgctattata
     3721 tcagctatta ttaatattat tagagtttta ataatattaa taa
//
LOCUS       DAFKMX010000264         3704 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFKMX010000264 DAFKMX010000000
VERSION     DAFKMX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.271"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            210..1790
                     /locus_tag="LZB08_003532"
     CDS             210..1790
                     /locus_tag="LZB08_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBM7316993.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1964..2410
                     /locus_tag="LZB08_003533"
     CDS             1964..2410
                     /locus_tag="LZB08_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBM7316994.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2431..3474
                     /locus_tag="LZB08_003534"
     CDS             2431..3474
                     /locus_tag="LZB08_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBM7316995.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1499 a          342 c          694 g         1169 t
ORIGIN      
        1 aataggtttt aaatcaacat aataacttaa attatatggt ataataaatt aaataattgt
       61 tttaattcaa aattttttgt tattttaaat tatttcaaaa ctcttagttt tagtgatttt
      121 atttatgata tttatgggat taaaaagatt tcaatagata tttttggtaa gtatttaaga
      181 agtctttaac tttataggag tgataattta tgctaatagg tacatctaaa ttaagcagaa
      241 atgttgatac tgcatgtgta gaagaagtag ggattccaat gattgtattg atggaaaatg
      301 cggttatatc tgcaatgaaa aatatggata tagacatgta taatagttat acagttgtat
      361 gtggtgttgg aaataatgga ggagatggtc ttggaatagc aagacatcta aatacatatg
      421 gaaaagaagt aaacgtattt ttagtaggta atcttgaaaa actaagtgaa tgctctaaaa
      481 taaattacaa tgtactttta aatatgggaa ttaatattat aaatattaat aaaaatgatt
      541 taagtgaaaa aaattatgga gatgaaaatg aagtagataa aattattaaa gttgagaaac
      601 aaaatattat aaaaaataaa aatttaacaa tttttaaaga tagagtatct aaaagtgatg
      661 ttgtagtaga tgctatattt gggactggat taaaacgtga tataactggt atttttaaag
      721 aagttattga catagtaaac gataatagca aaaatacata ttctatagat attccttcag
      781 gtatagatag cgataatgga aatgtattag gaacttgtat tagagcaaac aaaacgatta
      841 gctttgagtt ttataaaaga ggttttctaa actatgacac tagcacactt ataggtgatg
      901 tagttgtaga aaagataggt gttccagatt ttattacaag aaaataccat gatgaagagt
      961 tcataactga taagactttt gtaaaaaata atattagaag aagagataag catggtttta
     1021 aaagtgattt tggaaaagta tctatagttg caggttcaaa aggattttat ggtgcaagtt
     1081 tcatagcaac tgaatctgct gttaagagtg ggagtggact tgtaacatta attagtagtg
     1141 aagatgtgca agaaaaggta tctattagac ttacagaagc aatgacttta aattttgaag
     1201 aagaaagatt aaataaatta cttaattcta gtgatgcaat tggttttggt ccaggtatgg
     1261 gagataatag tcaaacattt gataaattac ttaagatagt agaaaattct aattgcccaa
     1321 tagtattaga tgcagatgga ttgaacgtta tgaaggacag atgttataag tttttagaat
     1381 ggaaaaatag atttgtaatt acacctcatt tgggagaaat ggccaggttg acaggagatt
     1441 caattgggta tataagagaa catagagtag atgttgcaaa agaatttgct caaaaatata
     1501 atctggtggt gcttttaaaa ggttatcaaa ctgtcataac agatggtaaa aaaacatata
     1561 taaatcctac aggaaatagc tgtatggcaa ctggtggaat gggtgattgt ttgcttggaa
     1621 tgattacttc atttataggt caaggtatgg atatattgga agctacagtt agtggagcat
     1681 atatacatgg ctatatagga gataaattga gcaaagcaat gtatacggta aatgctacgg
     1741 atttaataag taacatttca ttaacaatga aagagttgtt ggatatataa ttggtgatat
     1801 ttgatactac tttgtaacaa ttttaacaaa gtatgttgat ataatactat taattaatta
     1861 atttatttta ttgttttgta atttaaattt atattcaaaa acattgtata aacatataat
     1921 ttatataggg ttttataatt catatgaaaa gagggtagat agtatgaagt taatatggtt
     1981 gatagtagca gtcttatttg gaatagcaga aatgttaaca ccaagcttaa cattgatatg
     2041 gtttagtgta ggagctgtaa tacttatatt tttaagtagc tttatagaga gtattttctt
     2101 acaaatttta atatttgcag taatttctat agcaatgtta gtcgtagcta ctaaaaaaat
     2161 agtaaaaaaa gataaaggct ataaatctaa cactaattta caagcaatga tgtctaaaaa
     2221 aggtattgtt acagaagaga tttctccaaa taacacaggt ctagttgtag ttgaacatga
     2281 aacatggacg gctatttcta tagatggtga aaagatagaa aaaggttcta cagtagaagt
     2341 attaaaaatt gaaggggtaa aattagttgt taaaaaagta gacgccacag ttaatgtgac
     2401 aaaccaatag aataaaggag gatttatagt atgggtacta aaattgtttt aagtattgta
     2461 ttaatagtag tagtcgtagc aataagttta acttgtataa gagttataaa acaatctaaa
     2521 gtaggtataa taatgagact tggtaagttt caaaaagtgg ctgaaacagg tgtacatttc
     2581 ttaataccat ttttagataa aatggcatat gtaattgacc ttagagaaat tgtaatagat
     2641 tttccacctc aaccagttat aactaaagat aatgtaacta tgcagattga tacagttgta
     2701 tattataaag ttacagaccc agttagatat gtgtttgaga tagctaatcc aatagctgct
     2761 attgaaaatt taacagctac aacactaaga aatataattg gtgaacttga tttagatgaa
     2821 acattaacat caagagatat aataaatgta aaaatgagaa ctatccttga tgaagcaaca
     2881 gataaatggg gtataaaagt aaatagagta gagttaaaaa atataatgcc tcctcaggat
     2941 attcaagttg caatggaaaa acaaatgaga gcagaaagag aaagaagaga ggcaatactt
     3001 caagcagaag gtaataagtc agctgcaata cttcaagcag aaggtgaaaa acaatctgca
     3061 atattaacag cagaagccaa aaaagaagct atggtacgtg tagcagaagg tgaaaaagaa
     3121 tctgctatat tagtggcaga aggtgaagct gaagctataa gacagacagc tattgctaag
     3181 gcacaaggtg aagctgaaat gataaaaaga actcaaatgg caacagcaga aggtttaaaa
     3241 ttagtttttt cagcaatgaa agaggctgat atagacaaca atattctagc attaaaatct
     3301 atggaagctc ttgaaaaaat ggctgaaggt aagtcaacaa aactggtttt accttcagaa
     3361 gcagttaatt tcctaggaac attcaaagga ataaaggaag ttatgagtga tgataacaaa
     3421 gaagtacttg atataaaaga agttttaaat gataatgaat cattaaaaaa ataaaagagt
     3481 tttgttatca taggaaggat atggaaatta aaggtcgaat taattaaata tagcacaaaa
     3541 atgcacgttg aaaagaagag gacttataaa tttttaagtc ttcttctttg tttttattag
     3601 aaagatgaaa ttataaaaat aaattaacag aatctttata caatttatcc tttactaatg
     3661 taaaaagtgt tatataatat tatataaaac atatagcaaa taag
//
LOCUS       DAFKMX010000265         3655 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFKMX010000265 DAFKMX010000000
VERSION     DAFKMX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.272"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            169..1545
                     /locus_tag="LZB08_003535"
     CDS             169..1545
                     /locus_tag="LZB08_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBM7316996.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1856..2527
                     /locus_tag="LZB08_003536"
     CDS             1856..2527
                     /locus_tag="LZB08_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBM7316997.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2716..3513
                     /locus_tag="LZB08_003537"
     CDS             2716..3513
                     /locus_tag="LZB08_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBM7316998.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1388 a          402 c          674 g         1191 t
ORIGIN      
        1 atatataaaa atttaaaata ttccaaacag aaattataaa aattactatc attgtcttta
       61 tacaatattt atactataat agaataataa tttagaggaa agctctaaaa ataaacaaat
      121 taagggtctt aacagtagtg attttatgtt aaggaggtat aaagaaagtt gaaaacaata
      181 gttagaagac ttattctcta cgcagatgga atgcgtccta ctcaaataat ggtatctggg
      241 tttgctgcga ttattgtaat cggagcattg ttgttaacac tcccgatagc gtcgcagagt
      301 ggagagagta taggcttatt aaatgctcta tttacggcta cttcagcggt ttgtgttact
      361 ggtctagtta tggtagatac agcaacttat tggagtttat ttggacagat agttataata
      421 acattgatac aaattggtgg tcttggattt atgaccgtag ccacaatgtt ttcacttatg
      481 gcacgaaaaa aaatacaact aagagaaaga ttattaatac aagagtcatt aaatcaagct
      541 gatttatcgg gacttgtaag acttacaaga tttgtattaa taataaccat aacaatagag
      601 ggaataggtg cactggtttt atcaactgtc tttattccac aatttggttt gtctaaaggg
      661 atatggtata gtgtatttca cgcaatatca gcgttttgta atgccggatt tgacttgatg
      721 ggcagtgtaa gtggcccatt tacatcgtta aattcttatg taaacaactt tacagtatca
      781 atgactgttt gcgcacttat agtgcttgga gggctaggat tccctgttgt acttgatatt
      841 gtaagaaaaa gaagattttc aaaattaaat gtgcattcta aggttgtatt gttttcaact
      901 gctacactta tttttgtggg tgcattattt atttttctta tagaatttaa tcaaaaagct
      961 actatggcgg acttgccttt aaagggaaaa gtactatcag caatatttca atcagtaaca
     1021 gcaagaactg ctggttttaa tacgcttgat ttagctactt tacgagaaag tagtgtattt
     1081 gtaatgataa ttttgatgtt tattggagca tcacctgcat caactggtgg gggtataaaa
     1141 acaacaacac tagctgtttt aatcattaca gttagaagtt ttctatcagg aaaatcagat
     1201 atagaagctt ttgaaagaag attagctcca tcaactataa aaaaatctct tggtatattt
     1261 gtaattagta tatcagcagt tatttttgga acattgatta tttcaataac tcagcctaat
     1321 tttacattag ttcaatcagc ttttgaggtt acatcagcac ttgctacagt tggttcaagt
     1381 ttagctggaa ctccaaactt aaatgcttta ggtaagatta taataataat ttttatgttt
     1441 atgggtagag ttggttctct tactttattt atggctatac tttctggtgg tagaagaaag
     1501 agtcaaccaa tcagatatgc agaaggtaaa attatggttg gttaagttac caaggagtag
     1561 tataaattta taatggattt agagtagcac tgtaaatctg ttgataaaat aaatgagcct
     1621 agatgtaatt atatgaagaa ataaaatcaa ctaacgaaca aaattaatta atgaaataaa
     1681 aaaacaggga aataaaatac aaataaaaag gtaaatcaaa attaaattaa tctgtgtata
     1741 taaaattata tattgtgtat aagttttgta tgtgtaaagt atataagttt tgtatgtgta
     1801 aacaattaat ttaagagata ttatataaat tgtgtattag ggaggaagtt tcgagatgaa
     1861 gcaatatata gtcattgggt gtgggagatt tggaagttca gttgcgtcta ctatgcatct
     1921 tttaggacat caagtaatgg caatagacaa aaatgaagat tcagttcaaa gtatatctga
     1981 caaggtaacc cattcactta tagtggatgt tactgatgag caagcgttaa ggtcattagg
     2041 tttaggtaac tttgatgtag cagtagttgc aataggttct gatataaggg catctataat
     2101 ggcgactctt atagccaaag aaatgggtgt agagttgata atatgtaagg caaaggatga
     2161 attacaagct aaagttcttt ataaaattgg tgcagataga gttgtatttc cagaaagaga
     2221 tatgggagta agagttgcac acaatttagt ttcggataat atattagacc atattgaact
     2281 tgacccagag tattcaattg ttgaaatcgt aactccaaat agttgggttg gcaagacact
     2341 tatagagctt gaattaagag ctagatatga gataactgta cttgctataa aaacaggtaa
     2401 aaatataaat gttacacctt ctccagatga ggaacttaca gccggaagta tcctagttat
     2461 aatcggtcaa aatactagta taacagcgat aacatctgga aataagggga taattagaag
     2521 aagataattt actatttaat atatatttaa ttgcaatgaa agtagatagt atcatataat
     2581 tatgaatagt tatatgatac tattttttta atcgaaggta gtatttattt gtaagattag
     2641 ataaagagaa gttaaattaa aagtaaggag gctgtgctaa tataaaaatt tataagttat
     2701 tagcatttag ataaaatgat tacaaatata aacagtaagg ataatgaaaa gttaaagtat
     2761 acaagagcac tattaaaatc aaagaatagg aataaagagt caaagttcat aatagaggga
     2821 tacagaatag taatgcttgc acttgaatgt atggcaaacc ttgattatgt atttatcaat
     2881 gaagaatttg aaaataagaa agaacatgta aaactattag aagatttgga taaaaaaaac
     2941 acaaagatat acaagactac taataaaaac tttaaagaat tagtggatac agaaaatact
     3001 caaggaataa taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag tataaataaa
     3061 aaagataaat ttgtattgat tttagacaga atacaagacc caggaaatat ggggactata
     3121 ataaggactg ctgattctgc tggagtagat gccataatag cactaaaggg atgtgtcgat
     3181 atatacaacc caaaagtaat taggtctact atgggttcta tttttgatat gaatataatt
     3241 gatgcttcac aagatgaaac tgtggacatg cttaaatcat tggattttaa tatagtttca
     3301 agttacttaa atacagaaaa tttttatgac aaaatagatt atggctcaaa agtagcattg
     3361 gtgataggaa acgaagcaaa tggaataaat gaagaacttg tatcaaagtc tgatattttg
     3421 gttaagatac ctatatatgg taaagccgag tcgttaaatg ctgcgataag ctctgctata
     3481 ctgatgtatg aaataaaaaa atacttaatt taatgtattg taataaatat aatgtcatgt
     3541 tataatcttg aattaaatag tagagtatca aaaatagtca atatatatta aaaaaataat
     3601 taaattaatt atatattaga tgtatattat ataaaaaata attgagttaa ttatg
//
LOCUS       DAFKMX010000266         3646 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFKMX010000266 DAFKMX010000000
VERSION     DAFKMX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.273"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            218..1417
                     /locus_tag="LZB08_003538"
     CDS             218..1417
                     /locus_tag="LZB08_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7316999.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIVLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1426..2238
                     /locus_tag="LZB08_003539"
     CDS             1426..2238
                     /locus_tag="LZB08_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317000.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2249..3517
                     /locus_tag="LZB08_003540"
     CDS             2249..3517
                     /locus_tag="LZB08_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317001.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         1551 a          285 c          540 g         1270 t
ORIGIN      
        1 atgtaatttt aatcactttg ctgattatag caggcttata tttttcaaca atatatataa
       61 atataatcac tttatttaga aaatattata atcttatagg atttcaattt aacaatttag
      121 tagcagggtt attaagtttt ttaattataa tgtttattct aaatggaata tcttatttgc
      181 taattaaaaa gaaagtatct ctttaaggag gggtggtatg aaattaaaat ctaaaatatc
      241 tatacttatt atatttttga ctttagcaat atattttagt atagttttat taaataatga
      301 agataattta aagataaaaa ctataaaagg tgactctaat gaattagacc atataaacat
      361 aaattttgga tatgctttga atggatatat aaattcatat tatacaataa atggaagcaa
      421 caaaactaaa aagactgaat taataaaact acaaaaggat agtaagaaca caaataattt
      481 aattgatgta tctagtggtt cagatttaat tgctcatgtt ggatttgaaa tgaatgagga
      541 gtcaaaagat ataaactttg tgatagatga gaaaaaagaa gatttagaaa aaggtgtatt
      601 tccatcagaa ggtagtgata aagaaattga atatgaaaaa ttagatatga ataattttac
      661 aaggacaaga tataaagtag ataaaagtac agatgattat tatgcaaaga tactatcatc
      721 aaaaagatat aaaaaatact tatatgtatc tgtactttat gctacaagtg attcatcaga
      781 aaataacaaa gaatattcaa gtactataga tataataaaa attaatccag aaaataaaaa
      841 aatcaagact ataaaaagta taaatttaag taattatttt tccgaaaaat accaaaacat
      901 agagaacata ggtgtaagtt atgctagcaa atatgaggat aagatgtata ttttagtaaa
      961 tgtactaaat acaaacatag gtgataatag taattctaaa tttcatttac ttgagtataa
     1021 tcttgaagaa aataaggaaa gcataatgga attaaaaact gatagcaaaa tggctttagc
     1081 aaaagcaaag ttagaagcta ataaattata tatggtaatg tcgaatgatt atgataatag
     1141 ttcaattaca gagttaaatt tacttaccta taatatatta gataaaagct tagatgaaga
     1201 taaatatgtt ttagataata gagaaaaaag aggaattaga agtattgaaa ttgattcaaa
     1261 taaaatcgtt gcatatacat caaggctaaa ctctatatta tcttgggatg ataaatttat
     1321 gctatatata ataaataaaa attctaagga aattgtatat gaaggagaat tacaatttaa
     1381 cagttcaaat agtatgttag aatttgaaat aaagtaggtg agttgatgaa agatttaata
     1441 agattattcg aatttgaatt aaaaagaaat ataaagaatt atatatttat aataatttgt
     1501 tgttgttctt ttgtaatttt aaacatagta aaaaatttaa atgactataa ttacataata
     1561 gaaaatgcag ttaaaagtga acagttaaca aaaatcggaa atacgataga aacggtaaat
     1621 gttacaggtt tctcaagctt tagaaatata atgggttcta cagaattgtg gtttatgttt
     1681 ggtataattg catgtatttg ctatgctttt tttatttggt atagagactt taatggtaga
     1741 agtaaatcta tatatacact tataatgcta cctaaaaata gaatcaatat ttatatatct
     1801 aaattattaa atatattatt tctagtgtat agctatacag tagtacttac aatatctttg
     1861 tttattgcaa gtaagttgtt acctagacat atgcttggta atgtcacaaa ctatggtttt
     1921 gtacaagaaa caatatatga gttaaaaatg ttacttccat atagctttga aatactattt
     1981 gtagagtata tatttttatt gataggattt gtatctgtag tatttacatc aattcttata
     2041 aataaatcta tttataaagt aagcacttta ataagttttc tatttttagt tatagaaatt
     2101 ttagtgttta taatttctat agaatttatt ataccttacg aatacagtga tgtatttact
     2161 gttctatatt caagtataaa catatttgta tgtagtctta tatctaataa acttttgaag
     2221 caaaagattg acttttagga gggatattat gaaagcaaaa aatataggaa aatatacaat
     2281 atgttttata atagtatgtt ttttaatgat tactatgacc ttaatattga gttattttaa
     2341 taaggataaa ctatctgtaa aaactttaaa aggcaatgta aaagatatag ggagagtaag
     2401 tgtagtatat acacctaata taagcaaatt ttcttatgag gaggtaatta taagtaaaaa
     2461 tggagtaaga aataatacaa acccaaaatc aagaattaga aatgattatt taaatattga
     2521 caaaaaatac agtgactttg ttaatgataa aggaaaagaa tttatatata aagatgattt
     2581 gaatatagga gaagcgtata cctctgttaa gtctgattta gtacaaggaa tgaatgcttt
     2641 aaatgattgt ataaaggttt caaataaaaa tttaaaaagt ggggtagaag aaaattttga
     2701 tattaattta aataagtctg attttaaaga aaatagttgg aaaaccttat ttaacaaaat
     2761 atataaaggt aatttatata tggtaattag tgaaactaat atttcaggtt ctaaagtcaa
     2821 taatatttgt atattaaaga tagatataaa aaataagtct tataaattat taaatagatt
     2881 taatttagga ttacagaatg gagaacaaca aaatatcaat gaaattaaat tttctattgg
     2941 aagtaaatta tacttagaaa tagtaaataa taaaagtaaa aatggatata ttgaaggaca
     3001 tgatagttac tctaacttta ttatatatga tatagaagat aacactttta atataagtaa
     3061 taaatttatg gataatctaa acaaagaaaa gaatgcaaat ccaaaggaga ttggcttatt
     3121 aagtaaaact aagctagatt atatggtaaa aaacaataaa ttgaatctta tagttacgga
     3181 tatgagtaat tccataacta atcttgttta tgatattaat tctaatgaga ttaaattaga
     3241 agattatgag atacttgatt taaaagtaga ttataatgaa gcatcatctg gagttaggaa
     3301 agttaagcta ctggatgata agatatattc tatttcaggt attaagaaca gtatatcagg
     3361 agaattaaaa ggatttaaag ggattgttga tgtagtcgga actaagccag taaaatttca
     3421 agtatttgat atgaaactta aaaagaatat ttatgaagct gagctaataa atggagatat
     3481 aagtttacaa gaaaagttat tttttgttgc aaattaagtg ttagaattaa ttttagaact
     3541 ttaaactttt atatgtatca cagataagta taagtaaggg agtgtctcaa aataaacttt
     3601 ttagctcatg agacactttt tttatatgaa atcaaatata ttttca
//
LOCUS       DAFKMX010000267         3571 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFKMX010000267 DAFKMX010000000
VERSION     DAFKMX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.274"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            102..1598
                     /locus_tag="LZB08_003541"
     CDS             102..1598
                     /locus_tag="LZB08_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBM7317002.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            1732..2658
                     /locus_tag="LZB08_003542"
     CDS             1732..2658
                     /locus_tag="LZB08_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBM7317003.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNDVSQNGIQRSTKSSIKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            2776..2967
                     /locus_tag="LZB08_003543"
     CDS             2776..2967
                     /locus_tag="LZB08_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317004.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            3190..3540
                     /locus_tag="LZB08_003544"
     CDS             3190..3540
                     /locus_tag="LZB08_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317005.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         1430 a          345 c          673 g         1123 t
ORIGIN      
        1 atatgcctat gttataataa tttatagaaa taaataaaaa atacaatata cataatagta
       61 gaaatatagc aaaaaggtgt gatttatagg gaggattact tatgtttgaa tttgaaacgc
      121 aactaaaaag gttaaaacat gaagttttaa cagcagtagc aagactatct attgaagaaa
      181 atttaacaaa agaaaatata gaaaaaattc catatgaaat aattaaagga gaaaaggcga
      241 gatatagatg ttgtgtttat aaagagagag aagtagttct tgaaagggcg caacttgcat
      301 taagtttaac tccaaatagt aaatatggag atataaatcc agattcagtt agtttggaca
      361 cagatgagca aattatatat atagttgaag cagcatgtga tagatgtcca ataaatgaat
      421 ttacagttac agagctttgt agagggtgtt tggctcatag atgtaaggaa gcatgtaaat
      481 ttggggcaat ttcttatata aatgggagag catatataaa tcatgattta tgtaaggctt
      541 gtggaatgtg caaaagttca tgtcaatatg atgctatatc agaagttgta agaccttgta
      601 aaagcgtttg tccaacaggg gctttggatt ttaatagaaa cactatgaaa gcaatgatac
      661 atgaagataa ttgtataaat tgtggagcat gtatgtcagc atgtcctttt ggagcaatat
      721 ctgataagag tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa aatatgtatg
      781 caatagtagc tccagctata acaggtcaaa tagatagtaa tattacatat ggtcagttaa
      841 aaaatgctat aaaatcactg ggatttgtag atatgataga agcttcttgt ggagcagatg
      901 cagttacagt tcatgaaagc aatgaactta ttgaaagaat aaaatcagga gataattaca
      961 tgacaaattc atgttgtccg ggatttttta agtatataga gaagatgttc ccagatgaaa
     1021 aagatagaat atcatctaca gtgtctccta tggtagcaac aggaagatta gtaaaaaaaa
     1081 tagattctga tgctaaagtt gtatttatag gtccatgtac agctaaaaag agtgaggcaa
     1141 cagagaaaag tttaaaagat tcagtagatt atgtacttac ttttgaagaa ctaatggcat
     1201 tatttgaagc atttaatata gatttatcaa aatgttcaaa tgaagatata aatgatggtt
     1261 cgatttttgg aaggggattt ggagcatctg gtggacttgc caaatcagta cagaactata
     1321 ttgctgaaaa ggggcttagt tttgattttg aacctataaa agtatctggt ccaagacaaa
     1381 ttaaaaagac aatgacaatg gctaaattgg gaagattaga aggtaatttt atagaaggaa
     1441 tgatgtgtga agaaggttgt attaatggag caggtaagtt tttatcaggt gtaaaagcta
     1501 aaaatacttt tgatagaaaa aatggacaat ctactaaaaa gagtgtttta tctaatgata
     1561 agattaaaga atttgaagat gtaaatttag aaagataatt gttttggtac agtcattttt
     1621 ttattatgcc acaaatatgc ctaaaatttg ccctttttat atgtgtattt tacagagtta
     1681 aggttgttat actataaata tgtaataaat aaaatttata ggagcttttg gatgaggaaa
     1741 atagtattaa aagatatcct gtttttagtt actacattat ctataatagt aatattattg
     1801 cttgtaacaa atataactac taatagtaag ataaaatcaa caagtgaatc ttatttaaaa
     1861 agtgacaaaa atgatgtgag tcaaaatggt atccagagga gcacaaagag tagcataaaa
     1921 aatactgcat ttaagactac tatactaaac actggaaagt ctgattgcat attggttgag
     1981 ataggtaaca aagtcataat gattgataca ggagaagata aaaatggaaa acaaatagtg
     2041 gataggttaa aagaaaaagg aataaataca cttgactatt tgattttaac tcatttagat
     2101 aaggaccata taggtggagt agatagtgtg ttatctagtg ttaaagtaaa gaatattata
     2161 caagcaaatt ataagaaaga tagtaagcaa tatgatgaat atattgactc acttaaaaaa
     2221 gctgatatag aaccagtgct actaaaagat aatatgaata ttgtaatcaa tagtgcagaa
     2281 ataaacatac atccggcttc aaaaagcaag tacgagagtt caaatgatta ttcaataatc
     2341 actaatataa gctatggagc acataagttt ttatttgcag gagatgcaga ggaagaaaga
     2401 ttgtcagagt ttataaatgg aaatacttta aaatatgatt ttgttaaaat gcctcatcat
     2461 ggaagatatg acaaattaac tgaaacattt ttagagtcta tttcacccca atatgctgtt
     2521 attacatgtt cagagaaaaa agaaccagag gaaggtgttt taaaaatatt ggaaagattg
     2581 aatataaaaa catttttgac ttctaatgga gaggtagtta tcaatagtga tggtaagaca
     2641 ttgagtgtaa atcaataatt tttttaaaaa tatctaaaaa aatttaaaaa aatcattagc
     2701 aaatattacc ccttaaacat agtatatttt aggagctgaa actccacata caaaatgact
     2761 aggaggtaac atattatgtt ggatagattt tttggtgaag gcggatttgg tggatgtgct
     2821 tggtggataa taatattatt cttcttattc ttagcattcc aagattgttg ggctgaaatg
     2881 gacttatgtg catggatacc tttcttaata ttattattaa tactttgtga atgtggtgac
     2941 gtttttgatg gaggatgtgg atgctaaaaa aatcaattta gatgatttaa taaagcttat
     3001 ggtgggtata aataaaatac caaaccaatc acaatctagt gtttgaaggt agtcggaaat
     3061 cccccgctac cttctttttt ttattgttaa aatttatttt tgaattattt catttacata
     3121 taataatttt tacgataaag tctgatataa ttaaaaagaa aataaaattt atttatagga
     3181 gatgtaaaaa tggctatagt ggataagatt ttcaatgaga taaaacttat ggttgcaaga
     3241 cttttattgg gaaaaaaata cagtgaatat gaagaaaaag atttagtgta taatacagaa
     3301 gaagaagtta tacttataac tttaaaaaga ttagtttttc agggaaatgt aaacgaagca
     3361 gaagaaattt tatttgataa agctaagagt gtaaatagtg aaaatatgca gtatatagca
     3421 atagaatttt atactatgct tatggaaaag actgatgaag aattagaagc tatgaatttt
     3481 tctaaacaag aagtttatca aggtatagaa gatattagaa aagtattgaa tttagaataa
     3541 aagatatatg aaaatattga aataaattaa t
//
LOCUS       DAFKMX010000268         3565 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFKMX010000268 DAFKMX010000000
VERSION     DAFKMX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.275"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..227
                     /locus_tag="LZB08_003545"
     CDS             <1..227
                     /locus_tag="LZB08_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317006.1"
                     /translation="KDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLAT
                     GNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            214..969
                     /locus_tag="LZB08_003546"
     CDS             214..969
                     /locus_tag="LZB08_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBM7317007.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            1066..2103
                     /locus_tag="LZB08_003547"
     CDS             1066..2103
                     /locus_tag="LZB08_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBM7317008.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            2912..>3565
                     /locus_tag="LZB08_003548"
     CDS             2912..>3565
                     /locus_tag="LZB08_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317009.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     "
BASE COUNT         1331 a          472 c          544 g         1218 t
ORIGIN      
        1 tcaaagatac tactgcaata attgttggct cttttatata ccaattcact atatactttg
       61 caatgagttt aggaatgtta tcaactgact taaaactaat aactgccata gttataatag
      121 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
      181 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      241 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      301 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      361 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      421 ataacaaatt tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca
      481 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      541 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      601 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      661 gggggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      721 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      781 acagataaaa tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa
      841 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      901 gtaaaagata aagaaaaaga agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      961 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa
     1021 tatatgaaca aatataatta ataaatttta agggaggaaa ataaaatgat aaaaagtaaa
     1081 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1141 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1201 aatataggta ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc
     1261 atcaaagcac tcgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1321 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1381 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1441 gatattccga taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1501 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1561 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1621 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1681 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1741 ttagttggca aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1801 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1861 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1921 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1981 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     2041 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctgcaaaa
     2101 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2161 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2221 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2281 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2341 cagctttttt ttatgatatt tgattgatat gcatgtctag ttattccata tttagtaaat
     2401 attttgataa aatttccaca ttcacttata acttaggata acattttttt caaacaatac
     2461 attaatttaa aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg
     2521 acttttatta ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga
     2581 ctaattaaaa tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata
     2641 ttattattat tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga
     2701 ccccttaaga ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt
     2761 tttcatttaa ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac
     2821 cacatagttt tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt
     2881 ataaaaatat tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca
     2941 aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt
     3001 tccagatatg tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct
     3061 tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc
     3121 tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat
     3181 tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc
     3241 tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt tagccttagc
     3301 ttttgtattc atctgtaaag tccttttaga tttattccta aaaacaggtc ttggatacac
     3361 tttaaaaggt gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc
     3421 tataaaaata ctaggactta tgatttcaaa tggacttata gctttatctg gtagtcttat
     3481 ggctcaattc ttgggattct cagatgtaaa tatgggtata ggaactttag ttcttggaat
     3541 tgcctctatc ataattggta ttaca
//
LOCUS       DAFKMX010000269         3534 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFKMX010000269 DAFKMX010000000
VERSION     DAFKMX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.276"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            12..719
                     /locus_tag="LZB08_003549"
     CDS             12..719
                     /locus_tag="LZB08_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317010.1"
                     /translation="MKGFKTNVLKNISLTVNEGDFISIMGPSGAGKTTLLNLMSTLDK
                     QTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMKLMDNIALPLALGKKK
                     SKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLITNPTVIFADEPTGAL
                     DSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMINDGNIKCKLNKGNSRK
                     EFYGKIMDMLASMGGEM"
     gene            720..2627
                     /locus_tag="LZB08_003550"
     CDS             720..2627
                     /locus_tag="LZB08_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBM7317011.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            2779..3459
                     /locus_tag="LZB08_003551"
                     /pseudo
     CDS             2779..3459
                     /locus_tag="LZB08_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         1468 a          315 c          597 g         1154 t
ORIGIN      
        1 aagattatgg aataaaagga tttaaaacaa atgtattaaa aaatatatct cttacagtca
       61 atgaaggtga ttttatatca ataatgggtc catctggtgc aggaaagaca acattgctaa
      121 atttaatgtc tacacttgac aaacaaactt caggagaaat aatactagat ggaattaata
      181 tatcaaaagt aaaaaataat gaactatcaa aattaagaag agaaaaaata gggtttatat
      241 ttcaagacta taatctatta gacaatatga aactaatgga caatatagca cttcctctag
      301 cattaggaaa aaagaaaagt aaggagatag aagctaaggt attttctata gctaaaaaat
      361 ttggattaga aaatcattta gataaatatc catatcagtt atcaggagga caaaaacaaa
      421 gaggcgcagc agctaggtca ttaattacaa atccaacagt catatttgct gatgagccaa
      481 ctggtgcttt agactctaaa tcagcatatg aacttttaga atcattagaa aaaataaaca
      541 gagaaaataa cgctactatt ataatgatta cacatgaccc tctaacagca agttattcaa
      601 atgaagtata tatgataaat gatggaaata taaagtgtaa attaaacaaa ggaaatagta
      661 gaaaagaatt ttatggaaaa attatggata tgctagcctc tatgggaggt gagatgtaag
      721 tgagtatact tggaattgct tataataatt ttaagagtaa cataagaact tatctagcat
      781 tttttatatc tatggttttt tcagtagttg tgctaactaa ttttgaatta ttaagatatg
      841 gtgatgctat aaatgtgtta caagatgaaa ataaaaaatt tacactatca gtgttagtag
      901 ctgtaataat gatactatca gtatttttat ttttctttat atggtatgca acaaatatat
      961 tttttaagaa taggtctaaa gaaattggaa tattgtcttt tatgggatta gatttatata
     1021 ctataggaaa aatatatttt gtagaaaata tgcttatagg aataagttct tgtataacag
     1081 gaataattat agggataata acatctagat tttttcaagt agtgataata aagttatctg
     1141 gatttaatat aaaggtatct aatggactta gtatagaagc tatactatat gctactttaa
     1201 tctttatttc tatatttata ataatggctg taaaaggttt tataacaata tgtagaagta
     1261 gtgtaataaa tctaatcaat atatctaaaa aacaagaaaa aataccaaag gtaggaatag
     1321 gtatatatat attagctata gtatcaatta tagtaatagg atatggctac tatctttcta
     1381 ccaatataag aactggagat ataactagtg tactacctgt tataggtata ataatagtag
     1441 gtacttatgg attattcaaa tcagttatgc ctgttatatt tgatattata atcaaaaata
     1501 aaaaaatatt atttaatgga aataatataa ttgcaataaa caatataaac taccgattaa
     1561 ataaaaacta taaaacttat gctataatag ctataattat aactacaaca atatcaactc
     1621 ttggtgcttc agttgcgatg aagcgtatac ataagaatgc ccaagaacaa agaaatgtgt
     1681 acactattag catagtttca atggataaag atgaagtgaa taaaaaacaa atagagaata
     1741 taataaaaga tactaataag ataaagtact cagtaaatcc agaactaaca attttaaaga
     1801 aaacagatat aaaagaagct tatgatacaa cagatactat aatgaaatat agtgattttt
     1861 gtaatactct taaagtgaat ggcaatgata aagaattaaa agagtatggt gaaagattag
     1921 ttagtggaaa taatgtaata gagttaaaaa gccctcaaac gatagcatca atagctacaa
     1981 aagaagccaa gataaaactt gatggtcagt tatataatat ttcaagggga gaagtaaaag
     2041 ttccagtact agggacagga cttaatcaaa gtattatagt ggtaaatgat gatacatatt
     2101 ttaaactaag agataagggt caacaagtgt atttttatgg tgcaaaggta tttcaagaag
     2161 aaaatagtca agtaatgttc aacaatattg aaaagaattt aagtttaaaa aatgtatata
     2221 ttcaaaatgg atataaagct caaggtgaga gtcaatggat gaagtttgca tatgtagtgc
     2281 ttgtgttttt attcttagtc tttgcaattg tagctggaag tataatatat atgaaaatat
     2341 atagtgatgc atatgaagat aaagagaaat ataagatttt gttaaaaata ggtactacag
     2401 aaaaagatat aaatagagct atacttaaag aagtagctat tttttatact ttgccaatgt
     2461 taagtgcaac tatatctagt tattttgcat tgagattagc aggagattta ttgatgaccg
     2521 atttatttgg tatatatata ctaagtttag tgatatgttt gataatattc ataatatatg
     2581 gagcagtatc tgttaataag tttaagagtg tagtgtataa aaattagttg atatattaat
     2641 aaagagaaag gagattcttg taataagagt ctccttgttg attaactaaa taatttgggt
     2701 aataaattca tataaaatat gttatgatat taataattca tagcatagtg aaatattagg
     2761 ataatggaga atatacaaat gtatagaatt ttattagtag aggacgatat tgatttataa
     2821 aaagagattg cattagcact tgaaaaatgg ggatttaaag tagaattaat tgatgatttt
     2881 gaggtagtat tagatgaatt tatagataaa aaaccagatg tagtactttt agatgtaaat
     2941 ttaccattat ataatggatt ttactggtgc gaaaaaataa gagctatttc aaatgtacca
     3001 ttaatatttt tatcatcaag agattctgat atggatttaa taatgggtat aaataatgga
     3061 gcagatgatt acattaccaa accattttct atagaaatat tggttaccaa aataaatggt
     3121 ataataagaa gagtctataa ttatagtgat tctaatagta ttttatattg tgaagatttg
     3181 atgtttgatg tggggaaagg gattataaaa cataagtata aggataaaag tatagagttg
     3241 actaaaaacg aaataaaaat tttaacatta ttattaaaaa ataagaatag ggttgtatca
     3301 agggaaagtc ttatgatgac tctatgggat aatgatgagt ttgtgacaga taatgcactt
     3361 actgtaaata tgaatagatt gagaagtaaa gttaaagaac taggttttga tgattttata
     3421 aaaactaaga aggggatagg atatataata caatgctagt aaaatatttg aaaggtaggt
     3481 ctaagtatat tgctctacct ttagttgtag ttagtataat aaatatctac ttat
//
LOCUS       DAFKMX010000270         3467 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFKMX010000270 DAFKMX010000000
VERSION     DAFKMX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.277"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            236..613
                     /locus_tag="LZB08_003552"
     CDS             236..613
                     /locus_tag="LZB08_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7317012.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            complement(1050..1313)
                     /locus_tag="LZB08_003553"
     CDS             complement(1050..1313)
                     /locus_tag="LZB08_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317013.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            1629..2204
                     /locus_tag="LZB08_003554"
     CDS             1629..2204
                     /locus_tag="LZB08_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317014.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            2302..3300
                     /locus_tag="LZB08_003555"
     CDS             2302..3300
                     /locus_tag="LZB08_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBM7317015.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
BASE COUNT         1395 a          290 c          526 g         1256 t
ORIGIN      
        1 tttaaaaaat gtatatatta tgtcaaatat tagaatttta atttgataat ctgttatact
       61 tatagaatag atatttatct ttatattaat agattgtgtg tttaattttg aataaaaata
      121 taaatttttt gtattaataa atttggatga gatattttaa ataaaacctt tgactcgaaa
      181 agtctattaa tatagtattt aattatgtag aagtttgaga ggagtgtaaa atcatatgac
      241 tattactaag ataccaccag cagaattaaa agtcatgaaa tttatttgga gttcagattt
      301 tacagtagca tcaaaagatg tcattgaagc tatggaaaaa ctatataatt ggaagcaaac
      361 tacaacacta acacttttat caagattagt aaataagcaa ttcttaagtg caagaaaaat
      421 tgatagacat acacattata cagttcttat tggagaagaa gagtatttac attttgaaac
      481 aaaaactttt ttagataata tgcatggaag ttctattgag agttttatga aatccttgtt
      541 caaaaagggg gtaaatgaag aagaattgaa ctctgttgaa aaatgggtta aagatattaa
      601 aaaagatgaa taatttggtg tagttaatta tcataaaata aatagttttt acaataatta
      661 gttaaatgaa tcaaatataa agttttatgt aaaatttgtg atttttataa atatttttgt
      721 cttgttattt aatattaata caataagttt gatgtaaaat caaacctgtt tgaatttaat
      781 ttatattggg tttgtcatta atattatttt agtttgatat tgtatacgaa tttaaaagtc
      841 agaatttttg tatattaatc atgtaaatta attgttttag atataaaggg cataatttta
      901 tttttgtttt gattatttct agggtaagat aagtatacaa tagattgtat taaacatgct
      961 tatattgata gatttgagat aaatattgag atatattaat aattattaat ataaaaaagt
     1021 tcctagtttt taggaacttt tttatttgat taatacagag tatgagtttt tgcttcagct
     1081 atagctcttg ctgcactttt aggtttttca gtattttctt ttttgtaaaa tttaatcact
     1141 tcttctaaat ctttattttc aatattatgt tttttgtttt caagaatata atttacaatt
     1201 gattcattta tcaaatctgt caaaatatta ttttcgtcat tagttatctt agacatttct
     1261 tgtaataaaa tttcattaac ttctactgta atttgttttg tattttttat catatcaacc
     1321 acctcttaag aaaattatat catctcaaaa aaataaattc aatatgtttt aaaattatag
     1381 aattttaatt cttaaattct aaaaaagaga aaattagata aagtagtttt agattttaaa
     1441 agattaataa aaaatattta aagataaaag ctttattcta tatgattcta agatagagga
     1501 tttataagtg tttaaggtga attaaatata agacaacttc ttaaagcaaa tgggtgtggt
     1561 atttaaatct aaatgtagac atataatatt aaatatgcta atatatcaaa ataataaggg
     1621 gggaatttat gaagcttaaa gaagcattga ttacatgtgt tgtatgtagt gtatgtttaa
     1681 ctccattatt ggtaaatgga gaaacaaaaa tagatgcaga tcattgtaaa gaatctatag
     1741 taaatataga tactatagta aagtcaggta aaagctttaa ttccaagtat aatcaaggta
     1801 tggaagatta taaaaatagt gatgagtata agttgaaagt acaaaaggag aaactacaaa
     1861 atcagttagg tattaaaata aagaagttag tacctataac ttttgaagtt agttattaca
     1921 caagtcttaa tcaagaaaat ggatatggag cgataactgc tacaggagat gatttagaag
     1981 atggttttgt agcaaacaat cacttaagtt ttggaactaa gattattgta gatggaaaat
     2041 taaaaactgt tatggatagg gggtctagta agtattttag taattctaat gcaatagatg
     2101 tatttgtgcc caaatggagt aatgaaagtg aatcagagta ctatgataga gtcaataata
     2161 tgggaagaca ttacaaaaaa ggctacatta ttgttgaaga atgatgttct ttgaagtaat
     2221 atgtattgat tgtagtattt aatatatata taataaatat aaaactatta gtacaatgag
     2281 atatattact aggagggcaa tatggaacaa tcgtttataa atggaaaagt aaacttatta
     2341 atagaaaatt acaaagcatt aaatgaagtt aaaggttctt ggcaaatggg attgatacaa
     2401 catagttgtg cacttgcatt tacccttaag aacaagagaa taagtcctag attagtggaa
     2461 gaacgaatag agcttattaa aaaaaatact ggattattct ctaattttag aggatataat
     2521 atgttttata tggcaacact actttcgttt gaatcaaatc cagaaagtag ttttaagatg
     2581 attttagata tatacaaaga acttaaaagt gaaaagtttt ggggcgatac atatttacca
     2641 cttacagcat caatagttta tgaaaataga gagaaaatgg attatcttac atgtatatca
     2701 aaaatgaaaa taatatatga ttacatgagg aaaaaacatc catttctaac ttctagtgat
     2761 gactattgta atatagcact tattgcaatt cattcaaaaa atttagatga agatttagaa
     2821 tacatagaaa aatgttatga atttctaaat gaaaatggtt tttataaagg aaatgattta
     2881 caagcattat cccaaattct tttatttagt gatgatagaa ctatgcttaa atgtaaaaag
     2941 actattgaac ttaaaaaagc atttaaagaa aatgattgta aaatgaatta ttatggatat
     3001 ccaataatag gagcaatttc attattagat tatagagaag atgaaataat agaagagata
     3061 aaaaatgttt caaataaact taaagaagaa aaaggctttg gaaattggtc tttaggaaaa
     3121 agtaatagag taatgattag ttctgctatt gtagcttcaa tttatgcaga tttgatacga
     3181 aatgagaata atataggctc tataactaat aatatatttt taaatattgt tgttgctatt
     3241 caaattgcat gtgtaatggc agcaacaagt gcagctatag catcttcttc atcaaattaa
     3301 taagatttat tgaagttgga tatattaaat ttaataatac aatttatatg tttaaaaaga
     3361 agatagtatt acattttaat taactgtgat attatataat atgaaacata taattaatat
     3421 atagatttta ggcaataaat actacataca atatagggaa agtagaa
//
LOCUS       DAFKMX010000271         3437 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFKMX010000271 DAFKMX010000000
VERSION     DAFKMX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.278"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            158..346
                     /locus_tag="LZB08_003556"
     CDS             158..346
                     /locus_tag="LZB08_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBM7317016.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(506..658)
                     /locus_tag="LZB08_003557"
     CDS             complement(506..658)
                     /locus_tag="LZB08_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBM7317017.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(756..1232)
                     /locus_tag="LZB08_003558"
     CDS             complement(756..1232)
                     /locus_tag="LZB08_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBM7317018.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1451..2212
                     /locus_tag="LZB08_003559"
     CDS             1451..2212
                     /locus_tag="LZB08_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7317019.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2472..>3437)
                     /locus_tag="LZB08_003560"
     CDS             complement(2472..>3437)
                     /locus_tag="LZB08_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7317020.1"
                     /translation="SVLFDDLVLHFKSILNSKSYNLNKVNPLINTIKSNYPLAFEITL
                     NAIEKVFKNSIYSLTEDEIGYVSLHIGAGIERFFQSNIKCKNVVLVCGSGYGSSRLLE
                     VQLNKVFHDKINILQCLSFNQFLASELSDVDIIISTIPLNHDSIPIVLVDLKLLKKDI
                     ENISKSITNNSHIYSNLLDNFFDKNLFIVNPKIKDKDELIKLMCNKLTQSEIVFPSFA
                     ESVFYRESLSSTNIDDFLAIPHPMELSSIRTKICISILDEPIYWSEDSTVKLIMMLAI
                     NKDDYIKINSIYDILLKIIHDNDIRDSISNCNDFDNFLSIIKSIV"
BASE COUNT         1297 a          461 c          421 g         1258 t
ORIGIN      
        1 ttatataaaa ttatatatat aattttatat gataataaga aattcttcaa tatgtgtaag
       61 gcaaatatat atttctattg caaatttttg ttatttatga taatatgaat atgtttttag
      121 tctttaaata gttatcattt taaggaggtg tgcgtagatg gcaaaagtat gtagcgtatg
      181 tggtaaaggt aaggtttctg gaaaccaagt atcacactca aataaacata ataaaagaac
      241 atggtcagct aacttaagaa gcgtaagagc gattatagat ggagctccta agagagtaaa
      301 agtttgtact agatgtttac gttctggtaa aattgaaaga gcttagtaac cacgtttaaa
      361 agcctacttc cgtaagtagg ctttttaatt taatcatttt tactctttct cataaatttt
      421 aggattatac ttgaaatcca ttttggtaat tttattgtta ctactttcat caatactcac
      481 cccacacttt atagttaaaa ctattttaac aaaaaaataa taatgctcca catccaataa
      541 gtattaatgc ggtaagtgct gaccaaatcc agccaggaag caacattgac agaattacag
      601 ccattcctgt tgctagacaa agtattccta gcaatacctt agacttgtta tttctcatgg
      661 aacacctcca catagaacct gtatctataa atattttcat attcaagttt atgcgacagt
      721 tttccatttt agaccaataa acacattatg taattttaat ttttcatact agaattatta
      781 ctgaactccg atatagattc tacaatatcc aaaattcctt tttttctact taacctttca
      841 ggtccactat ataaaacaac tggtataact ttaggaagtt taaaattttt atcttctatt
      901 tcttcaaggc tttttttcat aataaaactt ttccaaattt gttctctata cgatttcaat
      961 cttgggacaa tttcagtatc catttctgtt tgaaattcta acactatata atgtgatact
     1021 ttgccacttt tagaaaatac cttataaatt atatcaactt cttttccttt atattcacat
     1081 ataaaagatg tttctaactc tatattttta agagttaatt cttttgctat tgagacactt
     1141 gtaaaatttt gaatcaaact taaaaaactt tctttatctg aatacattct tctgtactct
     1201 tctcgttttt cattgcttct ttttaaattc atacagttcc tcctagacta tagaccctct
     1261 attttttagt aaagatagtc tataaatttt attttttaat tttcattagt gagtattgac
     1321 agttgtgtgt aaaatatcca gtaaagatat ttttttatat ttttattaaa agatataaat
     1381 ttttatattt taatgtattg acatttacgt tgcgtaaaag tgtatattta atttatagga
     1441 ggtactactt atggaatata cagttcaaaa attaagtaaa ctagctggta taagtactag
     1501 aacacttaga tattatgatg aaataggtct tcttaagcca cttaaaataa attcttcagg
     1561 ttatcgtata tatggtcaaa atgaagttaa taaacttcaa caaatacttt tttatagaga
     1621 attaggaatt aacttagaaa atataaaaaa cattataaat tcaccaaact tcgatagttt
     1681 aagtgcgtta aaagaacacc ataataaatt actagccaaa agaaagcaaa ttgatttact
     1741 aatagaaaat gtaactaaaa ctatagctct gaaggaggga aaatacacta tgacagatat
     1801 agaaaaattt gaaggtttta aagaaaagat gattgatgaa aatgaaaaaa attatggaac
     1861 tgaaattaga gagaaatatg gtaaagatat tatagataca tcaaataaaa aacttaaaaa
     1921 tatgtctaag caagattatg aaaattggca aaacctaaat atcgaaataa ttagtaaact
     1981 taaaaaagct tttaaaactg gtgatgcctc tagtgaactg gctcaggaag ttgcaaaatt
     2041 acatcataaa tggttgagtt acacttggaa cacatactct aaagaagctc attcctctct
     2101 agcacagatg tatgtatatg atgaacgttt tacatcatat tatgatagag aacaacctgg
     2161 attagctaag tttttaaggg atgctatagt aatatataca agagataaat aatataaaat
     2221 aataaaggtg gtgtctcaaa atgaactttt tagttcatga gacactcttt tttgtatgaa
     2281 atcagatata tttttatcat tttatcaatg aaaaacaggt ttctctctct attttgagac
     2341 agaatcttta ttgtataata attataatca atacaaaata ataacaaatt atacaaaact
     2401 tacttatact atttatgcta aaaacaaaaa atattttatt aaagttttta aaacttatgt
     2461 aatataaaaa tctatactat agacttgatt atggaaagaa aattatcaaa atcattacaa
     2521 ttagatatac tatctctaat atcattgtca tgaattatct ttaaaaggat atcataaatg
     2581 gagtttattt ttatataatc atctttattt atagccaaca tcatgattaa tttaacagta
     2641 gaatcttcac tccaatatat tggttcatcc aatatggaaa tacaaatctt agttcttata
     2701 gagcttaatt ccataggatg aggtatagct aaaaaatcat ctatattggt agaagataaa
     2761 ctttctctgt aaaatacaga ttcagcaaat gatggaaata caatttcact ttgagttaat
     2821 ttgttgcaca tcaactttat aagctcatct ttatctttga ttttaggatt aacaataaat
     2881 aaatttttat caaagaaatt gtccaaaaga ttactataaa tatgggaatt attagtgatt
     2941 gacttagata tattctctat atcttttttt aatagtttta aatccactaa tacaatagga
     3001 atactatcat gatttaaagg aattgtcgat attattatat ctacatctga caattcacta
     3061 gctaaaaatt gattaaatga tagacattga agtatattta ttttatcatg aaaaactttg
     3121 tttaattgaa cctctaataa cctacttgaa ccataccctg aaccacatac taatactacg
     3181 tttttacact taatattact ttgaaaaaat ctctctattc cagcacctat atgcagggat
     3241 acatatccaa tttcatcttc tgttaaagag taaatactat ttttaaaaac tttttctata
     3301 gcatttaaag ttatttcaaa tgctaaagga taattactct ttattgtatt tataagggga
     3361 ttcaccttat ttaaattata agatttagaa ttaagaattg atttaaagtg cagtactaaa
     3421 tcatcaaata atacact
//
LOCUS       DAFKMX010000272         3405 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFKMX010000272 DAFKMX010000000
VERSION     DAFKMX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.279"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            226..771
                     /locus_tag="LZB08_003561"
     CDS             226..771
                     /locus_tag="LZB08_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBM7317021.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            784..1797
                     /locus_tag="LZB08_003562"
     CDS             784..1797
                     /locus_tag="LZB08_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBM7317022.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2019..>3405
                     /locus_tag="LZB08_003563"
     CDS             2019..>3405
                     /locus_tag="LZB08_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBM7317023.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFT"
BASE COUNT         1333 a          329 c          629 g         1114 t
ORIGIN      
        1 gaaataagaa ttgtatattt ctgaaaattc taattagtac aattttttcc tttataaaat
       61 tgaaaaaaga tgtatttatt tatcaaaaat attatattaa aatgatatta ataaaaaaac
      121 atttacaata ttagcatata gtacagaaaa aagatgtaat ttgtaaaatt tatggtaagc
      181 agtaaaatta aaaatttaaa gatattatag ggaggttata attttatgga aaacgttatt
      241 acaggtctaa gtaaaggtgc agagtggttt atcggtttgt ttcagaaagg gggagaacaa
      301 tttataggtc ttgtttcagg tactcttcca acgctaattg tattaatggt agctattaac
      361 tctttgatta agataattgg tgaggaaaga gttaataatt gggcttctaa gctaggaaag
      421 aactctttta caagatatat tctacttcca ttagtatcag tatttttctt gggaaatcct
      481 atgtgctata catttggtag atttttaaaa gaagagcaca aagcaggatt ttatgatgca
      541 gcagtttcat ttgtacatcc aattacaggt ctttttccac atgcaaatgc aggagaactt
      601 tttgtatggt tgggaatatc tgcaggtttg actactttag gtaaagaaac tactacattg
      661 gcattgtggt attttatagt tggtctaatt gtaattttta taagaggtat agtaacagaa
      721 aaaatgtatg cttttctaac aagaaaaaat agagcaacaa ataaggctta ggaggtagat
      781 aaaatggagg agaataaaat attaaaaata gaaaagggaa cttcaggctg gggaggtcct
      841 ttgtatataa aaaaagaagg gaatagaaat aagatactat caatgactgc tggtggtatc
      901 catgaagtaa cattaaaaat aaaagaacta ctgggttgtg aaattgtaga tggatttaaa
      961 actggagttt ctgatgaaga agtagcagta gttattattg attgtggtgg aactgctaga
     1021 tgtggagttt atccaaagaa aaaaatacct acaataaatg taaatccagt tggaaaaaca
     1081 ggtcctttag caaaatttat aacagaagaa tattatgtat cagatgttaa tcctaactgt
     1141 ataagtgttg tagatgggga agatatgcca caaaaatctc aagaaaataa aagcgaaaat
     1201 aagagttcta ttagaaagcc tgataattat gatgaggtta agtcaaaagc acaaggtgaa
     1261 tatgctaaaa agaacataat actttctatt ggtcaagggg cagggcaagt agtatcaaaa
     1321 ttttatgatg caggtcgaga tactatccaa atggtaatga ataacgtaat accatttatg
     1381 gcatttgtaa gtatgcttat gggaattatt ttagcatctg gactgggaga ctggattgca
     1441 agagttatat ctccacttgc aggtaatatt ggtggattat tgattatatc agtaatttgc
     1501 acacttccat tcttatcacc aatactagga cctggtgcag ttatagctca agtagtaggg
     1561 actcttgtag gtacacaaat tggtcttggt gcaataccag catatttagc acttccagct
     1621 ttatttgcaa taaatgggca agctggatgt gattttgtac cagtaggatt aagtttggga
     1681 gaagcagaac cagaaactgt agagtatggt gttccagctt tattttactc tcgtttaata
     1741 actggtccaa tagctgttat tattgcatat ggagttgcag tatttgcatt gagataatat
     1801 ttaatgtcaa tataaggttt ggaattaatt aataaaattg taaatataaa tttaagctag
     1861 tcaagatttt ttgttaatga aagactagct tttatactat taattagctg taattatcga
     1921 gttagattaa tactaaattt tcctttatta aagtgaaatt ttatgtatta atttggtttc
     1981 aaatttgtac tagaatgaaa tcaaggagga gatacaaaat gagtgtaact gagaaaattc
     2041 cttctattga ccgcaagata tttaagatat tgattatgtg tagtaaaaaa gagtttgtga
     2101 gtattaactc aatagcaaat gaattgaatg tcacaactag aagtgtaagg acgtatataa
     2161 aacaattgaa taaagattta ggtaatgata tagcagtaat caaatatata aagggtcaag
     2221 gctataaact agaaataaaa gatgagcaga tactcaataa gattattgat atgaatagaa
     2281 aaaatgtatt ttcattaaat tcaaaagaag atagagtaga gtttatacta aattacttga
     2341 tagaactaga tggatttatt acacttgata gtttagctga tgagatgtgt gtaggaagaa
     2401 ctactttagt aaatgatttc cagtatgtag aaaaagtttt ggcttcatat aatttaaact
     2461 tgattaaaaa acagaataca ggtatgaaat taaatggaaa tgaacttgat attagactgt
     2521 ttatattaaa tcagttgtat aaaaactcaa gaaaagattt tagtaattca aagtatttta
     2581 aaggaatcaa aaaagaagaa atcataaatt tagaagaaaa actattgact ttatttaaaa
     2641 aaaataattt ttatgttaca gatgaaatgt taagagaagt tattaactac atagtagttt
     2701 tagtctatag agtaaaagaa gaaaagaaag taaaagatta tgatgtaaag tttgatttac
     2761 ttaaatcata tgatgaatac tttattgcta gggaaataaa acatataata tcaggaatgt
     2821 ttgaatgtac tttaaatgat gaagaaatta tttatttaac tataccgtta gttagtggaa
     2881 atgcaccagc atcagaatgt gctttaaata gtagtagaat tagtaaaaac attgatgagt
     2941 tgatggaaaa tatatttaat caaatatatg tcgatatggg tatttttata aatgaggatg
     3001 aattaagagt aggtctagga tatcatctta gttttacttt aaacaggcta ttatttaata
     3061 taaagttaaa aaatgttttg ttagaagaga ttaagcaaaa ttatatactt ccatttaaat
     3121 tagcacaaat agcaggaaaa gtaatagaga aaaagtataa cttagaagtt tcagaagatg
     3181 agattggata tatagcaatc catttcagtg gatatttaga aagaaatagc agtagattct
     3241 attcaataaa aaaaatagca attatatgta gtacaggtct aggaacagca aaactattga
     3301 aaataagagt agaaaaactg ataggaaata atcctaaaat tgatactctt tcaagtttta
     3361 atttgagaaa tattaactta gatgaatatg atatagtttt tacaa
//
LOCUS       DAFKMX010000273         3391 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFKMX010000273 DAFKMX010000000
VERSION     DAFKMX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.280"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     misc_binding    298..508
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            734..1549
                     /locus_tag="LZB08_003564"
     CDS             734..1549
                     /locus_tag="LZB08_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBM7317024.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1577..2254
                     /locus_tag="LZB08_003565"
     CDS             1577..2254
                     /locus_tag="LZB08_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBM7317025.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2313..3035
                     /locus_tag="LZB08_003566"
     CDS             2313..3035
                     /locus_tag="LZB08_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBM7317026.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1365 a          338 c          625 g         1063 t
ORIGIN      
        1 ttattagaaa aagaaataag ggtttaatat attatttata gttgtttaga aaaagcaaca
       61 atgtggaaaa tattatttag tattattaaa gaaaagcaac aatgtgaaag atattattta
      121 gtattattta agaaatatct aaaaatatga aatatactat ttataattac acaaataata
      181 tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt aaaaaaataa
      241 aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt taaggaaaca
      301 aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg ttggtgtaag
      361 cctagcaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa ctttagtatg
      421 ataattggtt tacccgcctt aagggattga gaggataagt acttacttat caaaaagagt
      481 ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt tgattttaaa
      541 ataaaaatat ccatacagct taaaagctaa cagtcacaaa ttttgaaagt aatataagta
      601 tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag actgagagac
      661 tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata attaaaagag
      721 aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag tattaggatt
      781 gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag aacaaataaa
      841 agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg aatttcataa
      901 ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg aagaatttgc
      961 aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat taataggtgc
     1021 tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac cagagagaga
     1081 aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta tagttaaaga
     1141 tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag ttggagttca
     1201 agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa ctacaaaatc
     1261 tttgaaagca atacctgatt taattacaga attaaaaaat ggaaacatag atgcagttgt
     1321 tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa aaatggcaaa
     1381 tactgaagtt gggaaagatg taacagaagg tatggcagca gcaatcaaaa aatctgacaa
     1441 taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag atggcaagaa
     1501 gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt aagaaagtta
     1561 tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg ggacttcttt
     1621 tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct ttggatttat
     1681 aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa gagcaatatc
     1741 atcaatctat attgaggttt taagagggac accattatta gtacagatat atatagtatg
     1801 gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac caagtgaatt
     1861 tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag agatacttag
     1921 atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat taggattaaa
     1981 ttattggtca actatgagat atataataat acctcaagct ataaaaaata tattaccttc
     2041 attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg taataggagt
     2101 tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag cattagagcc
     2161 attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa gattagttgg
     2221 attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag catttaggga
     2281 aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat ttaagtaaat
     2341 cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa ggcgaaataa
     2401 tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt atgaatttac
     2461 tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc gataaaaaaa
     2521 ctaatattga tgaggttaga caaaatatag gtatggtgtt tcagaacttt aatttattcc
     2581 cacataagac tattttagat aatataacat tagcaccaat taaattaaaa aagatgacta
     2641 aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt ttagataaaa
     2701 aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca atagctagag
     2761 cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct cttgacccag
     2821 aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt atgacaatgg
     2881 caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt atttttatag
     2941 atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat ccaaaacatg
     3001 aaagaacaaa agcattttta gcaaaagtgc tataatatat aaaaaggatg ttattgatat
     3061 tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa ttaaagttat
     3121 attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc ggggtaagct
     3181 ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat ataaaatata
     3241 tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc agggggataa
     3301 ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga aataaattat
     3361 aaaagttatt tttataacaa gtaagcttat a
//
LOCUS       DAFKMX010000274         3384 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFKMX010000274 DAFKMX010000000
VERSION     DAFKMX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.281"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            121..1014
                     /locus_tag="LZB08_003567"
     CDS             121..1014
                     /locus_tag="LZB08_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7317027.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1192..2016
                     /locus_tag="LZB08_003568"
     CDS             1192..2016
                     /locus_tag="LZB08_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7317028.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2115..2723
                     /locus_tag="LZB08_003569"
     CDS             2115..2723
                     /locus_tag="LZB08_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7317029.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2827..3003
                     /locus_tag="LZB08_003570"
     CDS             2827..3003
                     /locus_tag="LZB08_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317030.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT         1252 a          399 c          471 g         1262 t
ORIGIN      
        1 aaaaatagga tattattata ggcaataact aacatcaagc gataacttaa tacatataaa
       61 aataaataaa caattaataa aaattaaaac ttcattataa aacggaagga tagtgaaaaa
      121 atgcaactat ataacacccc ctttaataca aacaaacact taatctattt ctttacattt
      181 acgctagtat tgaactggat attcagtctt acacctgcgt tgctcggaat tacaaataca
      241 atacttggag aaatcatttt ttatcttgca tatacatcac cagctattgt aggtctattt
      301 tttatttcta caatctatcc acaaagtgca aaacaagatt ttatttatag atacctaagc
      361 tttaaagaaa ttagaataaa atggatacta ttaacaatat tattttttgc tttaactttt
      421 attacttcgt taataatcgg aaattacttt aatgtaaaaa cttctgatat agattggata
      481 cacatagcag tgtttagacc ttctaaaata gcttatatgc tattagcaag ttttatattg
      541 agtatactta gtcaagaagc tggatggaga ggatatgcca ttgacaaatt attagttaga
      601 tttggattta taggctcttc aattatttta gggcttgttt gtggaatctg gtatctaggg
      661 tcgtatttta cacctaatca aataccatat aatttggcac aatactccct atttgatgct
      721 tttttattta taccaaacat tgtactttta aactttataa ttaattttgt atatatcaat
      781 acaagtagaa gcattttatc agctgggtta gttcatatga tgtactattt tttcaacatt
      841 caattacttc tacactaccc tattaaatta tgtgtaatag ctcaatatgt acaaataata
      901 tttggtttaa tatttcttgt atatatgatt tcttctaata aatttaaaca gaaggtaaat
      961 tctgaaattg aacagataaa gtcggatgat tttgaatttg agttagactg gtaattttaa
     1021 tatgaattat aaattaagat aatctatgat ttgatacgat tactacttaa atataattta
     1081 ctctctataa tttattgcaa catctggaaa ataattgtat ttccacaaac gcttgaagta
     1141 tataaaaaca aaatatatac tgattataag tgaaaaggag gtcatatttt catgtttaaa
     1201 ataggagagg tttcaaaact aacacaaatt tctattcgta tgttgcgtta ttatgatgaa
     1261 cttggtattt taaaacctgc aaaaacagac aaatatacag gtcatagatt atactcagtt
     1321 gaacagattt caatattaca aagaattgta ttattgagag attcaaaatt tagcgttgca
     1381 gaaatagcaa atatcgtaca taattggaat gatgaatttg taattaagga attgaataga
     1441 aaaaagaatg aaatacaaaa ggaaataaag caggaacaac aaagaataaa taaaattgat
     1501 aaatttatag aagctataaa ctgtgataaa gatgaaattc attacaatgt tgtctttaaa
     1561 aagattccaa gttataaaat catatcactt agagagataa ttccagacta ccaatctgaa
     1621 ggtatactct gggaaaagct atctaaattt atcaaagaag aacatataga agtatcacgt
     1681 caatccaaca ataacatagc tttttaccat gatgaagaag tcaaagataa tggtgtagat
     1741 attgaagttg gaatggttgt aaagaagatt ggaaagaaca aatctggatt tatatatcgt
     1801 gagacagaag aaattgatat gatggcatgt actatggttt atggtccata tgaaaatata
     1861 gctggtgcat atgaatcgtt ctgttattgg ttagataaaa atagtgatta tcaaatatca
     1921 ggtataaata gacaaatcgg tcataaaggt gaacataatg aaataaatcc tgaaaattat
     1981 cttacagaaa ttcaaatccc ccttataaaa gtctaatcga ttagtactta aatacttaag
     2041 tactaatcac ccattgacat cccaaaattt tcatgatagt atctaaacgt gtattaaaca
     2101 tgtttaattt ttttatggag aaacttttaa ggggaaaaat tatgaaagca gattcaacaa
     2161 aggaacaatt aattaatgcc acaatccagt tactatcttc acatagagat gtttcaagca
     2221 ttacggctag agaaattgta gctaaagcaa atgtcaattt agctatgatt aattattatt
     2281 ttaaatctaa agaggagtta attaatattt ctattaaaaa atttttaaag cgtcatatgg
     2341 atgaatataa ggctacacat gatactaaaa atttagcacc taaacagcaa ttaagggaaa
     2401 tactaattgc attttttgat tttataggtg aacatagtca atttataaag atttctgtac
     2461 cctatatatt gatacaggaa gaaattgttt atccatttga gatactacct ttaattaagg
     2521 ctcactatga aaatcagaat aacaaaaaaa ctgaagctga atacagaact attgcgtatc
     2581 aaataacttc atttattcag ttgctttttc ttcgttctaa tgcttttttc aagtatagtg
     2641 gtattgatat tcttaatgtt gaacagagaa atggtttaat agattttcaa ttagatttat
     2701 tattggtaga tattacaaaa taatactata ttaatttttt atgtacattt ttttatttta
     2761 tggtaacgtt tgaaaatatt ctcattgact aaaaatactt gcattaaata agggggagga
     2821 attttaatgt tttttacatt tataggagta atatcagcaa ttttattgtt gtgtacaatt
     2881 atatgtggtt tatggataaa acttaataat cgtaataatg actctaattc tcactttact
     2941 ttttcaatac tttcaattgc tttttcactg ttaattgtgg tggccttttt agtaagaaat
     3001 taatgtcttt gttatgctgg ggtttattat tatagatttt tattattgca tgatttttac
     3061 tatattatag tatttttatt atattacagg gtcttattta ttatagtgtt ttgttgttac
     3121 agcattttat tatattacag agtcctattt attataggat ttttattata ttgtaaaatc
     3181 ttatttatta taggattttg ttatattaca gacttttatt atattgcaat ctcttattta
     3241 ttgcagtgtt ttgttattac agtatttcta ttatattgca aactcttatt tattatggtg
     3301 ttttgttatt gcaacatttc tattatattg taaagtctta tttattacag ttctttatta
     3361 tactacagac ttttattatt acaa
//
LOCUS       DAFKMX010000275         3376 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFKMX010000275 DAFKMX010000000
VERSION     DAFKMX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.282"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            154..771
                     /locus_tag="LZB08_003571"
     CDS             154..771
                     /locus_tag="LZB08_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBM7317031.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(928..1380)
                     /locus_tag="LZB08_003572"
     CDS             complement(928..1380)
                     /locus_tag="LZB08_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBM7317032.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1828..3186
                     /locus_tag="LZB08_003573"
     CDS             1828..3186
                     /locus_tag="LZB08_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7317033.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
BASE COUNT         1320 a          334 c          578 g         1144 t
ORIGIN      
        1 agttgtatat ttttttaagg atataaagta aaaaaatata caatatcaag aaaaaaagat
       61 attgggtatt taaagtaata ctttatttaa ttttaagtaa atcggatatc tacatatata
      121 gatactaatt tcaaagaata ggagggtgtt tatatggaaa tattcgcaat aggacattca
      181 aattatccat atgataagct tattaatatg ataaaaaaat atgacataaa ttgtgtagtt
      241 gacattagag aaactccata ttctaaatat aatattcaat acaataaaga ggcatttaac
      301 gaatctctta gaaattccgg atttttatat atatacatgg gaaaagaatt tggagctaaa
      361 agaactaata aagatgtata tacgcaagaa ggttatgcag attttgagaa agtagcaaaa
      421 gaagatattt ttttaaacgg aatagaaaga ttaaaaaagg gttgtcaaat gggttatagg
      481 atagttttat taggagctat gcaagaacct ataagatgtc atagaagtat cttggtggga
      541 aaagtattaa ataaagaagg ttttgatgta aaatatataa tgcatgaagg aaatttggca
      601 tatcaggaag atatagagga atcattattg gataagtatt ttagtgatag aaagcaactg
      661 agtattgaca atttattagg gagtgcatta acgagagaag aaatgataca agaaggttat
      721 aagctagcta ataaagaaat aggatataga actgagaaac taggtaaata aaaatataca
      781 agtagataaa atacagtact tatcttaagt aaaataatat tatgaattta taatagctag
      841 gcatgatgtg ccaattaaaa atattctagt tatttaataa tatcattaaa tttagggtat
      901 tttttaatag cacatcatgt tgcagtttta aaaaataaaa tctctaatat tgccataatc
      961 aactgttcta ttaagagtag gatgactaga tataaatcta actgtaattt ttgaatttaa
     1021 caaacaagga tttgaaatta aaaatactct atctccattg acaatagaag taatagctac
     1081 tggcaaaata tctacaaatg tatcatatct gtttttagca atttgataag gataatttcc
     1141 tacaaaaaca aatagattta aatcatatat attatcagta aaccaagccc attgaccata
     1201 aactttgtcg atattcattg tgcctaaacc aagtgtatca aagtatgttg ctatagttaa
     1261 ataaatattt tccatgtttt ctaaatatga aacagtatat cttctgaaac gtatgggtgc
     1321 tgtggaagaa ataggataaa ccatatttac atttagatta tttgcattta aattaaccaa
     1381 agtatcacat cctaaaatta gtctataaaa tacaatattt aaatggatgc gattttgtta
     1441 catctaaagt gtaatatttt atatattgaa aaagtggtaa aagtttggat ggttaaataa
     1501 tattataaaa aatatataaa atgtacttat tgttattgct acacgatagt gatacaatat
     1561 taacgaaata tagctacttt taataataac ggaaaatatt tatattaaat taataaatat
     1621 tttaaatgtg gtatccataa aaaataataa acataagata attataaaga tatactgtgt
     1681 tcaaatgata ttaaacaaga tttatattat ttgaaagcag tatattataa tgcgaacaat
     1741 acaattctaa actttgcatt gtcaatttac agttcgataa actaattaaa aattaaattt
     1801 atataaagag ggaaaagagg aatagggatg tttataagga aaaatgttat aaagttagca
     1861 attcctataa tgatagagca gacgtttgtc atgcttttag gagtatttaa tacaatgatg
     1921 gcaggtcata ttggggaaga agctgtatct gcagttggaa tggttgattc aataaacaat
     1981 atatttatat ctgtatttgc tgcactatct gttggagcta ctgttgttgt agctcaacaa
     2041 ataggaaaaa aagatagtga aaaggtgaat gaaactatta agcaggctct agtgtctgga
     2101 gtaatattat cctttacaat aacaatattg atgtgggttt ttagaactgg tgtaattaat
     2161 ttattttatg gttccgcaga agaattggta aaatcaaatg caaaattata catagagttt
     2221 acattattta cgtatccatt tattgcgaca caacaaatag caaatggtat tttaagaggg
     2281 tgtggagatg taaagaaacc tatgtatgtg actatgttta tgaatatagt aaatgtaaca
     2341 ttgggatata tacttatata tggtattgat ttaaatcctc taggaataaa tttgcaaaca
     2401 ccatcctatg gcataacagg tgcagctata tctattgcta tagctagaat aattggatgc
     2461 atagtaatta taagagcttt atttaaagga aacgaattta tatctatggg taaaatattt
     2521 ccatttaaga ttgatgttga aactcaaaag tctatattta atataggaat accagctgga
     2581 gttgaacaac tactttttaa tgcaggtaaa ttgattgttc aagtatttat agttactatg
     2641 ggaacatcag caattgcatc caatgcaata ggaatgtcaa ttgcatccat aataaatgta
     2701 attgggaatg ctttagcttt gtctgctaca accttggtgg gacagtatat aggacgtgga
     2761 gatattaaag gagctaagag tactttatta tatttaacta aatttgcaac aatttgttta
     2821 tttatagtag gagctatatt tataccaatt gcaccctgga tttcaagtat gtatactcaa
     2881 aatgctagtg taattgacat ctcaacacaa ttaattcgaa gtgattgttt agctatggta
     2941 atatggccta tatcctttgt attatcagca gggctaaagg gagccggtga tactagatat
     3001 acaatgatga ctgcagtaat aggtatgtgg atttttagaa tttttacagg ttacgtattg
     3061 ggggttgtat tagaatttgg agtgttagga atttggatag gtatgtatac tgattggctt
     3121 gttagaggga ctttatattg tttaagatta agaggaacaa agtggctaaa acacaaagtt
     3181 gcatagaata tatgaaatca cttttagatg gataactcta tttagaagtg attttttatg
     3241 tagttattat cataattata agtatgatat ataaggtgat taaattacat aaataaaggc
     3301 tataatagta tggatattat aaattatata ttgagcagaa ttacataatt tggcgataaa
     3361 tattagaaat atatat
//
LOCUS       DAFKMX010000276         3352 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFKMX010000276 DAFKMX010000000
VERSION     DAFKMX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.283"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..440)
                     /locus_tag="LZB08_003574"
     CDS             complement(<1..440)
                     /locus_tag="LZB08_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317034.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFL"
     gene            complement(434..1159)
                     /locus_tag="LZB08_003575"
     CDS             complement(434..1159)
                     /locus_tag="LZB08_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317035.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(1161..1529)
                     /locus_tag="LZB08_003576"
     CDS             complement(1161..1529)
                     /locus_tag="LZB08_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7317036.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(1705..3249)
                     /locus_tag="LZB08_003577"
     CDS             complement(1705..3249)
                     /locus_tag="LZB08_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBM7317037.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         1106 a          559 c          314 g         1373 t
ORIGIN      
        1 tctaaaaagt tttcagaaat ccatttaaaa ctagtgttaa acaaatctaa aatttcaggt
       61 agtaaaattc cagaagtacc aaaaagcata agaactccaa aagtgaatac tgcaactata
      121 aaactaagta cagcgtatat aagtgctgtt atacatttag ataatataag tgaactagtg
      181 ctaacaggaa gagtaaacat taaataacct tcatccccca aaagattttt tctaaacctt
      241 tgaattgtaa ctacaatagt taaaactcct aatgccataa aaagacttgt taatactagt
      301 attcctattc cctgtacctg aagtatattt gtattcataa atattccatt aaatacagct
      361 actatcaata tggctatata taacggtata aatattcttc cacttgcttt taactcatat
      421 tttaataatt tacctaacat ttaaattcct ccctaaataa tgcatctata gattttcctt
      481 tttcttctct tattgcttca acgtctcctt gtaacactat ttctcctttt gatatgaata
      541 ttatctcatc acatatgttt tctatttcac ttattaagtg ggtagcaatc agtaaagtac
      601 tatcttcaga ataatttttt aatatagtct taagtatata tgaccttgca gctgggtcaa
      661 caccacctat aggttcatcc aatatatata tgctcgcatt tcttgacatt actaaaataa
      721 gttgaacttt ttctttagtt cctttagaca ttgtctttag cttttcattt acatcaatat
      781 ctaagctttt aatcatctca tttgctcttc taacatcaaa atcactataa aaatcataga
      841 aaaacttaag taaatctgat accttcatcc aatcatttaa ataagttctt tctggtaaat
      901 atgaaactat ttttttagta tctatggatg gtttcattcc atttatcata atttcaccat
      961 tatcaggctg taataatcca ttcattagct taatcattgt acttttgcca cttccattag
     1021 gtcctaacag cccgactatt tttccctttg gtatatttaa atcaatattt tttaaaacat
     1081 tcttagttcc ataactcttg tttacacctt taaattcaac tattccatct tgagaaacat
     1141 tgcttatatt tttatccatt ttataattct cctttcagat tttctgtaat aagttttagc
     1201 atttcatctc tatcatatcc aagcttttct aaggttactt ttaatgtatt tatctccaca
     1261 ttagctattt cctctttcat agcttttatt ttttccttat catctgttac aaatcttcct
     1321 ttagttctct gactataaac taaaccttgt ctttcaagtt ctgttagagc cttttgcatt
     1381 gtatttggat taacttccgc atcttctgct agtgctctca ctgtttctaa tttagatcct
     1441 attttaattt cccctgaaat tatttttaac tttaaatgtt ctactaattg tatatatata
     1501 ggtttgttat tatcaagttc ccattccatg ttcatgcctc cttattatat tattgtatta
     1561 atttgttaat acaataatat aataatacaa agtatatgtc aattactttt tagtaaaagt
     1621 ataaaaaatt ttacaaaaaa aatgaacact acattagagt ccatttttaa cacaatttat
     1681 taaattcttt atattacaga ttaattaccc tcttaccaag ttagatacaa ttaaatgttc
     1741 aaattcttca attggcattg gctttccaaa caaatatcct tgtgccatat cacactttac
     1801 ttcttttaaa agtttaaact gttcttctgt ctcaactcct tctgatatga ctttcatacc
     1861 caactctttt gccatttcta ttactttagt aacaattatt ttgcttcttt tactattata
     1921 agtttccata ataaattttt ggtcaagttt taagatatcc ataggaattt cttttaacat
     1981 atttaatgat gagtaccctg aaccaaaatc atccatagat attaaaaatc ctattttttt
     2041 taaattattc attatatcaa ttaaaatatc aaaattatca aatatgatac tctccgttaa
     2101 ttcaagttct atatactttg caggtatatt gtatttatca attatatttt tgtatgtttc
     2161 tataaaatta ttgcaatata aatgtatcct tgaaacatta actgaaacag taactggatt
     2221 tattcctgca tcaatccatt ttctaaggta tatacatact tcttcaagca catataaatc
     2281 taaatttata acaaaaccgt ttctttcaaa aaatgggata aattcatttg gaggtattag
     2341 ccctttatca ggacgtttcc acctaacaag tgcttctgaa ccttgtatct ctcctgtatt
     2401 aagttctatc ttaggttgta agtatataat aaattcttta ttttctagac ttgaaaacat
     2461 tgtattttcc atctcttttt cttttatctc ttggtcatga atcttattat cataaaatgc
     2521 atatgaactt ttatgtcctc cctttgccat tttatgaggt atattagccc tatctatgat
     2581 tgagttgata tctctatctt ctggaagtat aaaatagatt ccacaactta aaactaactt
     2641 atatttttct tcttgattat tattaaacaa gtctaattct tcaaatatag tttttaacct
     2701 ctttgttata tcttcttctt ctatatattt cataattata ataaaattat ccgcagatat
     2761 tcttgcaaac atttcctctt caaatatatt actcttaata atctttgtta aattacgcaa
     2821 aatttcattc ccaacttcat atccaaataa atcatttata tatttaaatt tatcaatatc
     2881 tatatataat aaagcatact ttacttgagt atttttacat agtattttgt tagcatcaat
     2941 tacaaattta tctatagtat ttgctcctgt aagattatct gtatatgcta tcctttgcaa
     3001 tttaatttta aaatttaatt tatcatatat tatatagaat ataagtatta cacatattat
     3061 agccataata agtattattt gtattgcatg tttgctaata agctgattat tagacacaat
     3121 agactcaaag ttatttatgg agttgttttt atagagtttg cataaagaat agactggtgt
     3181 tgtgtatgta atttgtaaaa ttatacaaat aaataaaaaa aacatcaatt ttttttgtaa
     3241 attcctcata aaaatttcct cccattaaaa cacctctatt ttattagcaa ataagtacat
     3301 ctcaaaagtt tccatttctt gtaaaaatgg atagttttga aaagaaaata tt
//
LOCUS       DAFKMX010000277         3288 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFKMX010000277 DAFKMX010000000
VERSION     DAFKMX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.284"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            939..1700
                     /locus_tag="LZB08_003578"
     CDS             939..1700
                     /locus_tag="LZB08_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBM7317038.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1765..2547
                     /locus_tag="LZB08_003579"
     CDS             1765..2547
                     /locus_tag="LZB08_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBM7317039.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2814..3233
                     /locus_tag="LZB08_003580"
     CDS             2814..3233
                     /locus_tag="LZB08_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBM7317040.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
BASE COUNT         1334 a          320 c          571 g         1063 t
ORIGIN      
        1 acaaatttgc ttatataaaa atcaatactt gaattattat ttttatgtga aatttatcta
       61 tagtatattt ttataaacaa gtaatgttat taaaattaag tatatagtta aaagtgtata
      121 gcaaaaaggc tgttatatta atgttagagg ttcagggaaa atatataaag caaagatatt
      181 tacagataat gtagattgca cagctataag ttagataata gaattatgta atcaagaatt
      241 tacaacaaag tataagataa ggataatgct aaattgccat acaggtgcta gatgtgtaaa
      301 tggatttaat taatactatt gataaagtgc aacaagcaca taaaccaatt gatgagatta
      361 ttaaaactat aagagatata gttgacataa ttaaaatatt ggaacctatt tataatttta
      421 aatcaaatca ataatgatta agattaaaat aatattaacg catcaatgtt ataagagtac
      481 aatataagga gtactatata gtactcctta cttatacata ttaggcaata gggaattttt
      541 ttatagttaa attttcaaat attttttagg gaactttata attgagaatt aagggtatag
      601 tatgtgatga acttactaaa tttataatta ggttatataa tggaataatc atcaatagtt
      661 ttactttagg gaaaatatat gcttaaggaa gtatagctta ctaatattat taaaatgtat
      721 atttaaaatg ataaaaaaag aattagacaa gtatttagta aattgattat gcaaaattct
      781 atcaagcagg aaccctatga ctttagtcat gggaggttca gtaggtaact tgttgaatta
      841 agtttatatt atttaaataa actacttaat ttagataata taaaaagtca gaattttaag
      901 aatagagcta tataatctta gctattagga gaagtactat gataggaata tattttagtg
      961 gaacaggaaa ttcaagatac tgtgtagaaa aatttttaca agaatatgat ataacagcaa
     1021 attcttattc tattgaagat aatgaacttt tacagcacat caataatcat gagaatatta
     1081 tttttagtta tcctgtgcaa tatagtaatg tgccaaaaat attgaaagat tttataataa
     1141 atcattcaac tttgtggaat ggtaaacgtg tttttattat tgcaactatg gctctattta
     1201 gtggagatgg ctctggtgtg ttatctagaa tattaaaaaa atacggtgct ataactgttg
     1261 gtggattaca tgtgaagatg ccagatagta taggagatga aaaggtatta aaacattctt
     1321 tagagtacaa taaaaagctt gtaataaaag cagaaaagaa agtaactaag gctgctaaaa
     1381 aactgaaaca tgggtcacca ccaaaagaag ggttgggttt tttgagtcat attgtagggt
     1441 tatttggaca acgattgtat tttatcaata aaacaaatat gtatacagat aaactaaaaa
     1501 ttaatactga ggaatgtatt gtatgtggta aatgtgttaa tttatgtcca atgaagaatc
     1561 tagtaattaa aaattgtatg attgttgcaa atgggcaatg tacaatgtgt tatcgttgtg
     1621 taaataattg tgccaaaaaa gcaattacat tgttaggtaa aaaagttgtt gaacagagta
     1681 atattaaaaa atatatgtaa gaaatttaaa gttttattga atgtaaattg atgtatattt
     1741 tatgttagaa agagaggtta aataatggaa tactacatag ttgattcatt tgcaactaaa
     1801 ctatttaaag gaaatcctgc tggagtttgt gttcttgaca gaagaatacc tttagagtta
     1861 atgcagaaaa tagcagaaga aaacaattta ccagaaacag catttgtagt taaaggtaaa
     1921 ggaaattatg aattacgatg gtttactcct aaagcagaaa ttgatttatg tggtcatgca
     1981 actttagcag cagcttatgt aatatcaaat tttattgatg ttaatgtaaa aaagatagat
     2041 ttctttactc aaagtggaaa gcttgaagtt acacgaaatg gaaatctata tgaaatgata
     2101 tttccagaaa taatgcctat tgaaatagag ttatcaccgc aacaagctaa tttgataggc
     2161 tgtgtaccaa gtgctgttta ttcttcaaga gatttaatct tattgctaaa cagtgaacaa
     2221 gaagttataa attataaacc taattacgca caattgcgta aattgacaga ctggttaggt
     2281 ataataatca cagcacaagg aagtaataca gattttgtat caagatattt ttgtccagaa
     2341 ttagattcag aagaccctgt tactggttca tcacattgta atcttattcc atactggtct
     2401 gaaaagttgg gaaaacacaa aatggtagct gctcaacttt ctaatcgtgg tggtataatt
     2461 cagtgtgaag tgttgaagga taatactgtg aaaatttctg gtgaagcagt cttgtttatg
     2521 cagggaacaa taaagattga tatttaaata agtataataa ctaatttatt atgaaaaata
     2581 attaattttc ttagtgttaa caataataaa gtagagttaa aaaacttgat ttcacttaaa
     2641 tcaatatgat agatgttgat actaagattg acaagcatga ttaaaaaaga taatatttaa
     2701 tctataaaga taaaaacaca gtctggttgg tagtccagac gtagcaatgg ctgtcagtaa
     2761 cctgcctcct tggttgtccg ttctttagaa agtaatttaa ggaggaacta gcaatggaca
     2821 aaattagtgg aaaattaaca gtcttatttg aagaaccatt ttgggtaggt atatttgaaa
     2881 gacaagatgg taaaaaatat gaagcatgta gggtagtttt tggagcagaa cctaaagaag
     2941 tagaagtgta tgaatttata cttgaaagat ttttctcatt agattttggt agcattaaat
     3001 tagaaaaaaa tgtgaccaaa gacaacattg gatacaaaag aatgcagaga aaagtaaaaa
     3061 aggaacaaga aaaagaaact atagggacta aggctcaaaa tgctcttaag cttcaatatg
     3121 aagaaagaaa acaggataga aaaaacctag ctaaaaatag aaaagaagaa gaaaaagaaa
     3181 gactatttaa tttgaaacaa gaaaagagaa aagctaaaca taagggtcat taattttaat
     3241 taaatatctt tttaataatt gctagaaaaa ggtgtataaa atttttaa
//
LOCUS       DAFKMX010000278         3245 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFKMX010000278 DAFKMX010000000
VERSION     DAFKMX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.285"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(279..536)
                     /locus_tag="LZB08_003581"
     CDS             complement(279..536)
                     /locus_tag="LZB08_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBM7317041.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1116..1337
                     /locus_tag="LZB08_003582"
     CDS             1116..1337
                     /locus_tag="LZB08_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBM7317042.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2155..2385
                     /locus_tag="LZB08_003583"
     CDS             2155..2385
                     /locus_tag="LZB08_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBM7317043.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2398..2547
                     /locus_tag="LZB08_003584"
     CDS             2398..2547
                     /locus_tag="LZB08_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBM7317044.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2804..3241
                     /locus_tag="LZB08_003585"
     CDS             2804..3241
                     /locus_tag="LZB08_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBM7317045.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1313 a          415 c          350 g         1167 t
ORIGIN      
        1 atttatatag tttatttatt tatttttccc ctttatacaa gcaattgtat cattttatct
       61 attttttagc caacaaatat tttctaccat tttctttgta ttctcaatat atctccagta
      121 tttaacaaat ttttttatag aatatacaat gtatttaaat taataagtag taaatttagt
      181 taatttgtct aaaaaaactt tataaaacat cttctttgtc tttactctta cgtttaacaa
      241 ggctattttc attatgttta aacattattt tattatattt aaacactatt ttcattgagt
      301 attcgcttaa tattgcttac acacatatta tatctaatag ccaattcatt gtggttgact
      361 ccattaaatt gttttactat atccctattt ctagcatatc taaaaagccc cttatacgta
      421 ggtatataag tatttgttcc tccatacaac ttggcaacct ctatgaactt atcaattccg
      481 attatttcta ccattgcatg aaattgaact ggtatatcct ccacttttat cttcataagt
      541 tacacctcct ttaagatatt tttatagact taacaaagtt tttatttaat acattattga
      601 ctaaactata tttattagtt ttattgaatc taataaataa tttcttagct ttaaaattaa
      661 tttttgtatt aactttaata aaatttctca taacttataa ctttgttagc tttaataaag
      721 attttaatgg tttaaaagta aaatgttaat attggacttt taactttttt atttacagat
      781 taaatttaat aaaattttta acataagctt taacatttaa tttaacataa actttagtac
      841 ctaacattta actctacaca tatgaaaatc catacaaaac tatctcaact aagcaggaat
      901 atatacaaat actatttcaa aacactaaga acatatcttc acaaataact tgtaaacttt
      961 ttcatcatca aaaaagtaaa atttttccta taaaaattta cacaaaaaat ctttagaaat
     1021 tactgtttaa ctccgatatt atcttagatg atagtattat agctaacaaa gaaagtcgac
     1081 aaatctttga aattaaattt ttaggaggta attttatgga agttgtttca acaaaaaatt
     1141 tatctagtat aaaactaaaa ttggatgctg gttttgatga taagggtaag gcagttgtta
     1201 aaagtaaatc atttgcaaat gtaaaagcag aggcattaaa tgaagatgtt tatgctgttg
     1261 cagaagcaat agcaagtctt caagagaacc ctattgtaga tattttaaaa ttagatagta
     1321 catcattatc taaatagtac cttttaaacg tactctgctt tacaaattaa aaaaattaac
     1381 tctttatata aaagattaag ttatataacg cacttaatac gctatagatt tggcagatta
     1441 taacttcaat tttgattata actttaatat attaatgctt tgaaaaatta atatattaaa
     1501 cgtatttcaa cttaaacatg ttagctattt tagatgctac cctactttaa tctaattaat
     1561 tgaaataaat taaatatttt ctatatcagc aataactaga gtcaacaatt aaaatactaa
     1621 tattgacaat tgaaacactg atactaacaa gcaaaacact aatattgaca attgaaatat
     1681 taatactaac aagcaaaaca ctaatattga caattgaaat actaatacta acaagcaaaa
     1741 cattaatatt gacaattgaa atactaatac taacaagcaa aacactaata ttgacaattg
     1801 aaatactaat actaacaagc aaaacactaa tattgacaat tgaaatacta atactaacaa
     1861 gcaagacact aatattgaca attgaaatat taatactaac aagcaaaaca ctaatattga
     1921 caattgaaac actgatacta acaagcaaaa cattaatatt gacaattgaa atattaatac
     1981 taacaagcaa gacactaata ttgacaattg aaatattaat actaacaagc aaaacattaa
     2041 tattgacaat tgaaatatta atactaacaa gcaaaacatt aatattgaca attaaaatac
     2101 caatattaac aagcaaacaa ttaaaatata aattattata tcggaggtaa ttatatggaa
     2161 attaagaaaa aattagtcat gagctttaaa aatgtaaagg ataaacaagt ttctttttct
     2221 atagaaaatc caaaggatga tttaactgaa gaaaatataa aatctgtcat ggatttgata
     2281 gtatctaaaa atattttttc ggttggagga tttgacctag cttctgttgt tgaagccaag
     2341 gttgtggaaa caaataccac tccttatgac ttagttatag aatagggggc taattttatg
     2401 gatgatagtt tacaactgtt aataaccaat gttggttttc ctatagctct atccatgtac
     2461 ttattggtta gaattgaggg taaattaaat actttatcaa acagtattga tacattatcc
     2521 aaaaatattt tgatatttaa gaagtaattt gacaatatat gtaagtcata aaataggtct
     2581 atttgtaaaa taattagaca tattctatgt gaaatactag gatatttgga taatttatcc
     2641 aaatatccat agtatttttt tgaattttac cacctcaaat ttgaatttca ctgttttatt
     2701 tttgaatttc actatgctta atttgaaatt tagaacgcat ctagttggac tcaataagat
     2761 ataatgctat tctaatgaca gtaataaaat gaggtgatga ttaatgaaaa tacatgtcga
     2821 acaaggtatt aattgtgatg atattgaaat aaccataaaa tgttctgctc tagataataa
     2881 cattgaaaaa ataatttcat tattgagtac accgttactt gatataaaag gtaaaaaatc
     2941 aggagagatt tataaactca atgtctgtga tattttatac tttgagacag ttggaaataa
     3001 aacatttgct tattatgaag atgatgtatt tgaaattgat ttaaaaatat ataaattatc
     3061 aaaattatta gctaatacat ccttctcacg aatatctaaa tccatgattt taaatatgga
     3121 ctacataaaa agtataaaag taatatcaca taatcgcatg caagctactt taaaaaacaa
     3181 cgaaaaagtt attataaatc gtcgttatat aaaaaatatt aaagaaaagc taaacattta
     3241 ataaa
//
LOCUS       DAFKMX010000279         3197 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFKMX010000279 DAFKMX010000000
VERSION     DAFKMX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.286"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            117..764
                     /locus_tag="LZB08_003586"
     CDS             117..764
                     /locus_tag="LZB08_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBM7317046.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(931..1500)
                     /locus_tag="LZB08_003587"
     CDS             complement(931..1500)
                     /locus_tag="LZB08_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBM7317047.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1548..2405)
                     /locus_tag="LZB08_003588"
     CDS             complement(1548..2405)
                     /locus_tag="LZB08_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBM7317048.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFFKR"
     gene            complement(2416..2565)
                     /locus_tag="LZB08_003589"
     CDS             complement(2416..2565)
                     /locus_tag="LZB08_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317049.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2558..3142)
                     /locus_tag="LZB08_003590"
     CDS             complement(2558..3142)
                     /locus_tag="LZB08_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBM7317050.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1115 a          553 c          363 g         1166 t
ORIGIN      
        1 tcgtaatctt aggaaaaggt tacttataaa attaacctta tacaataata tatcgtataa
       61 tcttaattag cataatagtt acaatattaa aatttaaata tgggagtgat tttagtatga
      121 taagacaaac accattagag aaacgatatg acaagctagg tcctcatatt gtaacagcac
      181 tcaaaaaacg ttattttgat gcttattact gtaaaactag aaatgaagct ttgcaacaaa
      241 ttttagattt aattcctcaa aatcatgtag tatcatgggg aggatctgaa acacttaaag
      301 aaatgggcgt acaaactgct gttagagaga aaggatttaa agttattgat agagatttag
      361 caaaaactcc agaagaaaga actgaaatta tgcgtcaagc acttctatgc gatacttttt
      421 tgatgagtag caatgcaatt agtgaagatg gacaactctt taatattgat ggaaatggca
      481 atcgtgtagc tgcaatgata tttggtccaa aaaatgttat agttgtagct ggtatgaata
      541 aaattgtaaa aaacattgat gatgcaatac aaagaactag aacaattgca tctccagcag
      601 ttgttcagcg ttttcaagat gtcaatacac catgctatat taatggaagt tgtatagact
      661 gcacaagtcc tgaaagcatt tgtgcataca tggttactac aagaatcagt cgtccagcaa
      721 aaaaaataaa ggtaatattg gtaggagaaa atcttggtct ataaaatata catacatgta
      781 aatagctcta taaataataa aatcatatga aataacttat ttctaaaagt ggtagaataa
      841 aagcttctac aagactttca ttctaccact tttatattct tttaatttgt gtttctatat
      901 tattaattgc tccgattttc atatatatta ctaactattt ttattttcat aatttttttg
      961 ttcttcatca agctgtttat aataatcttt attatagtca gctagataat ctataccacc
     1021 tcctgtaatc acatttaata atttcattgc agattcttga ttaactttac aaacataatt
     1081 atcaccagat ttaacaagct ctaatgtaat attaaattct tttttctttg cagaatcaat
     1141 atctttcata gttggatttg gcatcatttc tcctagttta ggaggattct tttttaaaat
     1201 agaaaccata tcagtattgt taatttttaa atctacagtc actttatcct tattattctt
     1261 tatatctgaa atttcccaag tcatattttt agaatacatt ttaaatcctt ctttatcttc
     1321 atatataaga ttacctaacc ctgattttgg gaaatactta tcaacagtat ctgcatcttg
     1381 tgtttttatt gaattaaaca tactatccac ttcatttttt atcttattat cattagtaca
     1441 accagctaac accagtactg atattgcaaa cattataata catataataa atttcttcat
     1501 taaattatat ccccctatac ttttttgcac aaatattatt ctctttttta tcttttgaaa
     1561 aaagttgtag taattgcatt ttgtggacaa acttttatac attcaccaca cctaatacag
     1621 tctggactat ttacatcttt atatattggt atatcaagct tacattttgt ttgacatgct
     1681 gtacaatttg tacatttatc tttatcaatt ttaaatctat atattgaaat aggattaaaa
     1741 aaaccataga ttgctcctaa agggcatata tacttgcaaa atggcctata agtaatgatt
     1801 gacatcaata atatcaatag caaaatactt aacttccagc taaataaaaa tcctattgat
     1861 tctcttaaat tctcatttcc taaaataagt ggtattcctc ccaaaagtgt tcctgaaggg
     1921 caaatccatt tacaaaacca aggatttcca ccccctgcta catttacaat agcaactggc
     1981 aaaataatta caaacattat taagactata tactttagat attttagata tttatcacca
     2041 taaacttttt ttatctttac tggaaagggt actttatgta gtaaatcttg tacaagtcca
     2101 aagggacaaa gccaaccaca aacaaatctg ccaaaaaaag caccaaatgc cattaaaaaa
     2161 ccaacaacat aaaatgagaa tttatactct ctgcttgata aaactgcttg taatgaacct
     2221 attggacatg ctccaagtgc acctggacat gaataacaat tcaatccagg tacacataat
     2281 tttttacttt ctcctccata tatagttcct tctaaaaatc catttacata tccatttgtt
     2341 aatgctgtaa atgcaacttg agtaataagt cttatattat tatttacttt agataataac
     2401 ttcattcctc ttctcctaac caataccaat acactcaaga caaatcataa ttgctttagt
     2461 taatactgta tttgtttctc cacgatatgt acctaatgca ataaatataa tactaattgc
     2521 aataatagaa atgccttttt tattatcttt aataaaatta ctcattggct tttaccattt
     2581 ttagtctttc atctataatt tttttccaat cttccttact atttgcacct acaaccttct
     2641 cacccacaaa gtttcctttc ttgtctacaa aataagttgt tggaaactcc ataactcttt
     2701 taaaatcagt ttttttaagt tcttcagata cagtcaattg ttggtaagtt gcatctgact
     2761 ttttcataat gtctttaact aaatttttct cttcatttga aagaccagtt ctcatatcag
     2821 ttttatcaac ttctacaact aatcccttta tagctacatt ggaattatta ctctcatatt
     2881 cttttgataa tgcttcaagc tctggcattt cacccacaca tggtccacac caagtagtcc
     2941 agacattaat aagagtaagt tctttatttg aaaatatctc ttttgttacc ttatttccat
     3001 caacatcctc cgtatctaaa ttactaaacc attcactatt attcttgttt ttttctactg
     3061 gcttagattc ttttttgcta catccagaaa tcatacctaa aacacaaata catcctaaaa
     3121 ctactatgaa aaaccttttc atatctcaca ctcctagaat ttatatagta tataaaatcg
     3181 ctgcttgaaa taaaaat
//
LOCUS       DAFKMX010000280         3178 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFKMX010000280 DAFKMX010000000
VERSION     DAFKMX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.287"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            322..2964
                     /gene="adhE"
                     /locus_tag="LZB08_003591"
                     /gene_synonym="adhC"
     CDS             322..2964
                     /gene="adhE"
                     /locus_tag="LZB08_003591"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBM7317051.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLNAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1219 a          399 c          618 g          942 t
ORIGIN      
        1 aaatttaata agtaaaaaaa atgaaagata taaattatca ttttagtatt ttatttagtc
       61 gaagtaaatt attcaaatga aatatatctc atttagataa aaaattttag taaaagtaaa
      121 aaaaataata tatttgttaa aaaataaatt gacaaaatat tcagaaaaaa acttcttgac
      181 ctaagagagt aaaataaata aaatctaagg tatagagatg aaatctataa aattatttta
      241 agtataagtt aaaaatatag atgatgcatt taagatttat aaaaattaga gatgcataat
      301 tattttaggg aggtttttag tatggagaaa aaagaaaagg tagtagaaaa atcaaatgtt
      361 gaagtatgta gtcctgaaat tgtcaacagt gttgaaacat taagaatgag attagaagaa
      421 attagattgg cacaaaaaga atttgcaaca tttacgcaag aacaggtaga taagattttc
      481 ttagctgctt caactgcagc taatcaacaa agaatacctc tagcaaaaat ggcagtggaa
      541 gagactggta tgggtattgt tgaagataag gttataaaaa accattttgc ctcagaatat
      601 atctataatg cttataaaga tactaaaact tgtggtgtca tagaaaaaga tgaagcattt
      661 ggttttacaa gaattgctga gccagtaggt gttttatcag cagttatacc aacaactaat
      721 cctacatcaa ctgcaatatt caaatcccta atagcactaa aaactagaaa tggtataatt
      781 ttctctcctc atccaagagc taaaaactgt actatagaag cagctagagt tgtacatgat
      841 gcagcagtta aggcaggagc accaaaagga cttataggtt gggtagatgt tccttcgatt
      901 gaattaacta atgttgtcat ggcagaggca gaccttatat tagctactgg tggacctgga
      961 atggttaaat ctgcatactc ttcaggtaaa ccagctgttg gagttgggcc tggaaatgta
     1021 cctgcaataa tagatgagag tgcagatata aaaatggctg taagttcaat cttagtatca
     1081 aaatcattcg ataatggtat gatatgtgct tccgagcaag ctgtaatagt accagaaaaa
     1141 atatatgaag aagttaaaaa agaatttaaa tatcgtggag cacatttcct aaataaagaa
     1201 gaaactgaaa aagtaggaaa agtagtaata attgatggtt cattaaatgc cagaatagtt
     1261 ggacaaccag cacatgtaat agccaaaatg gcggatgtag aagttccaaa aacagctaga
     1321 ataataattg gtgaagtaga atctgttgaa ttaaatgaac cttttgcaca tgaaaaactt
     1381 tctcctgtac ttgcaatgta caagtctaaa agttttgaag atgcagttgc taaagcagaa
     1441 aaattagttg cagatggagg ctatggtcat acatcttcat tatatgcaga ctctataaat
     1501 catccagaga gagtagaaaa gtttgtaaat gctatgaaga cttgtagagt attagttaat
     1561 actccatcat cacaaggtgg tataggtgat ttatataact ttaaattggc accgtctctt
     1621 actcttggtt gtggttcttg gggtggaaac tcagtatctg aaaacgttgg agtaaaacat
     1681 ttgcttaata tcaagacagt agctgaaagg agagagaata tgctttggtt cagagcacca
     1741 gaaaaggttt atttcaaaaa aggatgttta ggtgtagcag caagagaatt taaggatgtt
     1801 atggataaaa agaaagcatt tatagtaaca gattctttcc tatataataa tggatatact
     1861 aagaaattaa ctgatttatt agatgagatg ggaattaagc atactacatt ctttgatgta
     1921 gctccagacc caacattagc ttgtgcaaga gaaggagcta aagctatggc tgatttccaa
     1981 ccagatttaa taatagctgt aggtggagga agtgcaatgg atgctggtaa gataatgtgg
     2041 gtaatgtatg agcatccaga agtagatttc caagacttag ctatgagatt tatggatata
     2101 agaaaaagag tatatgtatt tcctaagatg ggtgaaaaag cgtattttgc tgctatccca
     2161 acttcagcag gaactggttc agaagtaact ccatttgcag ttataactga ccaagattca
     2221 ggtgtaaagt acccattagc agattatgaa ttaatgccaa atatggctat aattgatgct
     2281 gatatgatga tggagatgcc accaagatta acagcagcat caggtgttga tgctttaaca
     2341 catgcattag aagcatatgt atctatgtta agaactgaac cagctgatgg tctagcacta
     2401 caagcaggta aaataatatt tgaatattta cctcgtgcat ataaaaatgg aaagaatgat
     2461 aaagaagcaa gagaaaaaat ggcaatggca tctactatgg caggaatgtc ttttgcaaat
     2521 gccttcttag gaatatgtca ctctttagca cataaattag gtgcattcca ccatgttcaa
     2581 cacggtgttg caaatgcatt attaataaat gaagtaatca agttcaattg tgcagaagct
     2641 ccaaataaga tgggtgcatt ctcacaatac agatatcctg attgcataca aagatatgct
     2701 gaatttgctt cttttgcagg tattaaagga agtacagacc aagaaaaagt agataatctt
     2761 ataaaagcaa tagatgaact aaaggcaaaa gtaggtcttc ctaagactat aaaagaggct
     2821 ggagtagaag aatctaaatt cttagaaaga ttgaatgcta tggttgaaca agcttttgat
     2881 gaccaatgta caggtgctaa tcctagatac ccattgatga gtgaattaaa agagatatat
     2941 ctaaaagttt attatggtaa ataatatcta ttgtgcaaca taggttctat tgggtttgta
     3001 tataaattat tagatttgaa tataaataga gttttattag agacacgtag aagtaactat
     3061 aaataataga ttataactta aattctaagt ggaaatgata aaaagtcagt aatttaatgg
     3121 taatttcgaa tatgtgtatt taatcaatta actttaaaat tagtcggaaa attttttt
//
LOCUS       DAFKMX010000281         3128 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFKMX010000281 DAFKMX010000000
VERSION     DAFKMX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.288"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(488..1240)
                     /locus_tag="LZB08_003592"
     CDS             complement(488..1240)
                     /locus_tag="LZB08_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBM7317052.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1299..2954)
                     /locus_tag="LZB08_003593"
     CDS             complement(1299..2954)
                     /locus_tag="LZB08_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317053.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         1079 a          516 c          257 g         1276 t
ORIGIN      
        1 tttaaagtta tgtaaaatta aaaatacagg aatgaaaatt ccattcctgt ataaaatcta
       61 acttttgagt ctcagtacaa tcttattttg agacaatctt ttttgtttta tattttctta
      121 tatttaaaat ataacgcata tcttatgcaa catattcata tttttcttta tattctttca
      181 tcgcatcatt ccaagcgtct tgtactgtta cacctctttt attagcaatt ttttgtgcca
      241 gttcaattat cttcatagct cttcttgata tgttcataaa cattcctcct acaaattacc
      301 atatatttca tatatataaa tcaaggttat atttttatga tatataatcc ttctattgta
      361 agattacagt ttatatgtgt attatatatg aactatatta gaagataatt agaattttta
      421 taaagctata tgattagatt aaatataaaa gctttacaat tacttataaa tttaaaaagt
      481 ctataagcta aactaatgtt tgatatacca attttacgac cataaataat gccattacta
      541 aaaatattgg ttttatcatc tttgaacctt tctttatagc aactttagca cctacataag
      601 ctcccattat catcactaat gcaaacacta tagcaatttt ataatctact tgaccattta
      661 tcatgaataa taacaatgaa gttatattac ttgttaagtt taatattttt gtatttgcag
      721 aagcatgtaa aaaatcatat ccatatattt ttataaatcc aaaagtcaaa aaagttcctg
      781 tacctggacc aaaaaaacca tcataaaaac ccattataag agccataagc atccctagtc
      841 ttaaattttt cttattaaca gcttcaaaat tatcttcttg acctaaattt ttagaaataa
      901 aggtgtaaat agccactatt aaaatcataa ccacaaccaa aacatttaat atctcttcac
      961 ttattgataa tacacatctc acacctagaa cactaccaat aatagtaaat ggtactaatt
     1021 tttttaaaag gttattatta atttttccag actttgcata tctataggca ctacttatac
     1081 atcctgctga tgctgcaaat ttatttgtac caatggcaag atgtactggc actccaattg
     1141 ccataagaac aggcatgctt ataagtcctc ctcctcctgc tatagaatct acaaaagcag
     1201 ctaaaaaccc ccctatacat aaaaaaataa ctgaaaacat aaaattcctc catcattctc
     1261 tttcaatttt tcttacaata tatattatat aatcttattt aatacttatt atccagcata
     1321 ttctttctat attcatttgg agttatacta ttaaacttct taaatatttg tgaaaaataa
     1381 ctactgttat taaaacctac tttcttacaa atatcactaa tactcatgct tctatctaac
     1441 agatattttt tagattcttc tatcctaaga agatttatat actcagttat tgaaacttta
     1501 tttctttcat taaaaataga actcaaataa tttggactta taaaaaaaac atctgatata
     1561 ctattcaaat ttatattttc tttataatat ttttttatat attcgtttat attttcaatt
     1621 agatttcctt tatcttttct ccaaaatgct ctattcttat caatacactc tttggaaaat
     1681 tcaaataata cattccataa atcttcatca ctgtttacaa gtgatatttt tttattatat
     1741 ttatttctta aataagatat aagttttaaa tcctctaaat ctctaagcac tgttctagac
     1801 aatacaaata tcatttcctg aatagtatct ctaacatcat tatatcttaa tttgtttgta
     1861 tctattagat tataaacacc ttctaaattc atatttttaa tttctttttc aatataaagt
     1921 tctaattcaa agggatattc ttttgaagaa ttattgaaca tatctatcgg attaataata
     1981 tactcttctt ctaataatct actatttgct tttttaagtg cctcttcaag tcttatattt
     2041 tcttttctac tcttcaatat ctcctcatct tttatactaa gttgattttc aaaaaaattc
     2101 ctcttagcta tttcctgaat aaattcagac atcattaata aattttcttt taatacatat
     2161 aatctacttt taggatttaa tggaaattca ttgtaaattt tgtttatgta ttttatatca
     2221 ataccagaac tacttgataa ttcagataaa tttttatcta tataaataga cttatatact
     2281 ataaaattac cacattctat atatccaact atattttctt ctaatactat aggtatctct
     2341 aaactggaaa caccatactc acaatgatac actttttcaa agttatcatt atttatgctt
     2401 ttaacaagtt ttttagtaca cggttttata tttttacaaa tatcgcaaaa tttattttct
     2461 ttcttagtct caaatatttc tttatacttt aagtcatata cagatattct aagcttcaat
     2521 atattagata tttgatttgc aagattttca attatgttta cattaactaa ctcctgtata
     2581 ttgttttcag aaaatactat aggtatttgt ctcaaactcg aaaatttatg aggaacataa
     2641 gtaattaata ttactaaatc aattttacca gtgtttataa tttcatgttc tgaatatggt
     2701 gggcaataaa ctatagatgt ttcctttata ttgacctttt taccatcaat tatttgtatt
     2761 ccttctccct gaatcacata taaaagctgt tcttcactta aatgaaaatg cttttcttgg
     2821 cagtttcccg gaaaaaatct tacaattcca gcactaagtc tattgaattg tgtttttgat
     2881 ggttcgtgta tccacataac ctctccccaa tcaaattttt gtacttcatt atttgactca
     2941 ataaaatcca ccataaactc acctcgtagt ttttgtctaa atattttttc ttcccttctt
     3001 atattaaaat aatatcatca tcattaactt tcattactat aaaatcatca tactctttta
     3061 atactcctgt aattttagtt gcacttagtt tactacaatt cagtttttta atatattgat
     3121 aaattcta
//
LOCUS       DAFKMX010000282         3096 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFKMX010000282 DAFKMX010000000
VERSION     DAFKMX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.289"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            375..1652
                     /gene="hflX"
                     /locus_tag="LZB08_003594"
     CDS             375..1652
                     /gene="hflX"
                     /locus_tag="LZB08_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBM7317054.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1885..2382
                     /locus_tag="LZB08_003595"
     CDS             1885..2382
                     /locus_tag="LZB08_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBM7317055.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2432..2956
                     /locus_tag="LZB08_003596"
     CDS             2432..2956
                     /locus_tag="LZB08_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBM7317056.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1285 a          266 c          540 g         1005 t
ORIGIN      
        1 tgtaaataac tacttaaatt taagaatagg gttaaatata aaatgataat tacatataag
       61 ttaattaaac taatgtattt ttaatatata tttagaattt taatccttct tatgatataa
      121 gtagcttgtt ttgtattact tagatttgta aggagttttt ttattgaaaa aactgcataa
      181 tatatttggg aggaggtgta agaaagtgat tttttatata agattgagaa aataatgcac
      241 gattatggtt attataatag taaataagaa gtgtcattta aggataagat aaaaagcaaa
      301 attcttgatg aatttaatta tagagccata atctcgtgct atagattaaa taaaaaatag
      361 cagataagga gaatatggat atggcgaaaa aaggaataac agtaggatta aacataaata
      421 ataaatctga ggactttaat gaactaatga ttgaacttga aaacttatgt tcagcatgtg
      481 atattgatgt tgtagggagt ataactcaaa atgcaaagca ggttaataga gcattttaca
      541 ttgggacagg taaagttgag gaaatattaa acttaataaa gaaagaaaat atagagatag
      601 ttatatttta caatgaactt tctacatcac aactaaaaaa tcttgaagaa aagcttaatt
      661 gtgaaattat agatagaacg gcattgatac ttgatatatt tgcacaaagg gcaaaaacta
      721 gagaagctaa acttcaagta gaagtagcaa gtttaaaata tatgttacca agacttatag
      781 gttcaaatga aaatttagga cgtcaaagtg gtggagttgg tactaaaaat agaggttctg
      841 gtgagaaaaa attagaactt gaccgtagaa gaatagaaga aaagataaca tctttaaata
      901 gggagttgga tgatttaaaa ttccaaagag aaactcaaag aagtatgaga agaaaatcaa
      961 atcttccaaa tgtagctcta gtcgggtata caaatgctgg taaatcttca ataatgaata
     1021 aactagtaga tatatttaaa aactctgaag aaaagaaagt gtttgagaaa aacatgttat
     1081 ttgctacact tgaaacttct gtcagaaata tagttcttgc aaataacaaa gaatttttat
     1141 tatctgatac tgttggtttt gtaagtaatc taccacatga cttagtaaaa gcatttaggt
     1201 caacattaga agaagcatgt gaggcagacg tactgttaca tgttattgat atttcaaatc
     1261 caagctataa aagtcatatt aaagtgacag aagatacttt aaaacaaata ggtgcagatg
     1321 gtatacctat gatacatgtg tataataaaa ttgatttaat agatgtagaa gtattggata
     1381 gaattttaga tagtatcgat aaagaaggta tatttgtttc agttaaaaaa gatataaata
     1441 tagataaaat gataaaatgt atttgtgata gcatatttaa agattatgta agatgtaagt
     1501 tcttaattcc atatgataaa ggtcatgtag tttcttattt taacgaaaat acgagcatta
     1561 taaatacaga atacagagaa gatggagcta ttttagatgt agaatgtagc aatatagaat
     1621 ataataagta taaaaagtat gctttagagt aaattttgaa aaacttatta tagcctaaaa
     1681 tatatttaat ttataattta ttataaattg attgtaaata tgattttatg aaacggtata
     1741 gccataatgc tatgccgttt tgccatttat aataaaaata aatttattga cataatagta
     1801 actaattact ataatataaa gtaattagtt actatttaaa attatcaaaa aattaaaatc
     1861 taagataaag agaagtgtat aataatggat gcagaaagtt taatgaaaaa atatatggta
     1921 gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca aaataaactt
     1981 caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt acttttagct
     2041 attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt aatggggtgt
     2101 tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt tatagaaatt
     2161 gagcaaaatg aaaaagattc aagagctatc tgtcttgtga caaaagatag agtgcaagag
     2221 tattctaaaa aagtaagagg tatgcaggaa aaagtattac agcttttatt tgaagatttt
     2281 acagagaaag aagtggaaca gttatttttt ggaattatga aattaagtat aggaattgaa
     2341 aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat caaatcaact
     2401 ttgtatgaca tcagagaggg gggtaattta tatgaaaaat gtagtagtga tttataagag
     2461 taagtatggt tcaacaaaaa aatatgctaa atggctttca gaattattgt cttgtgatat
     2521 ttttgaaact aagaatatta atatagaaaa ggttatgaag tacgatacga ttattttagg
     2581 aggaggtctt tatgcttgta atatagcggg aatttctttc ttaaaaaaaa attataaaga
     2641 attgcagaaa aaaaatttag tagtatttgg cgtaggagct gctccatatg atgatgaagt
     2701 aataaagaaa ttaagagaag taaactttaa ggatgaatta aacaaaattc cttgctttta
     2761 ttgtcgtggg gcatgggatg agacaaaaat gtcttttata gattatactc tttgtagttt
     2821 ttttaaaaaa tctatagcta aaaaagaacg tcaaggtctt aaaccagcag aagcaccttt
     2881 attgtctgga gaagcctttg attggtgtaa taaagaaaat ttaactccaa tacttgatta
     2941 tataagagaa ctttaagagg tatatatttg taattattta ttatctaaag ttagatttca
     3001 tagaaattta ctagacatta tttataaaat aatattatta aagatggata atttaattgt
     3061 ttcaataagg ttataatttt aaaaaatgtt tatttt
//
LOCUS       DAFKMX010000283         3074 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFKMX010000283 DAFKMX010000000
VERSION     DAFKMX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.290"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            286..2421
                     /gene="rnr"
                     /locus_tag="LZB08_003597"
     CDS             286..2421
                     /gene="rnr"
                     /locus_tag="LZB08_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBM7317057.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1354 a          273 c          556 g          891 t
ORIGIN      
        1 atttgatggt gtataattag gtgataaaga gtaatttaag caattatgat aaagaataac
       61 ttaagtaatt ataatgatat ttacaaataa tttataaaac atcataattt gtttgaagtt
      121 agtatgacaa agcttacatt aaatcaaaaa tataaataag taattttaac ttataataat
      181 aagaaaatat agaaaattat gtgataagta aaaaatgttt tgtctgaaga aatataaaag
      241 acaatataaa taataatata atgaaaagta aaacaggagg gaaaaatgat accaggactt
      301 aaagaaagtt tgctaggatt aataaatgaa agtgcatata atcctctaaa aaaagaagaa
      361 ttagcagaaa tatttgatat acattcatca gaaatgccaa tgttttataa ttttttaaat
      421 gaattagaag aagatggtta tatctgcttt actaaaaaag gaaaaatagt atctcccaat
      481 caaatgggat actttgttgg aaaatttgta tcacacaaaa agggttttgg atttgtagaa
      541 tctgatgttg aatatacaca ggatttattt atctcaagtg acaatataaa tggtgctatg
      601 cataatgata gagttatggc agaaatagta gtacctgcaa ctgaagaaaa gagagcagaa
      661 ggaagaatag taaaagtaat aaagagagaa ataactagaa tagtaggtac tttccaatca
      721 agtaaaactt ttggatttgt aacaccagac aacaagaaat ttactaaaga tatatatata
      781 cctaaaaaac attttagtgg agcagtagat aatgataagg tagtatgtga aattacagta
      841 tggccacaag aggatagaaa accagaaggc aaaattatag agatactagg tcaaaaaggt
      901 gaaagaggcg tagaaataga ttctataata agagaacatg gtcttccaga agaatttcca
      961 aagaaagtat tgcaagaagc agaagctgta gctgttgaga ttcctcaaga agagataaaa
     1021 agaagaagag atttaagaga tttaaacata tttactatag atggagatga cgcaaaagac
     1081 ttagatgatg ccatatcaat agaagtatta cctaatggaa acttcaaact aggagttcac
     1141 atagctgatg ttacacatta tgtaagagag aaaaataaac ttgacaagga agccttaaaa
     1201 agagctacat cagtttattt agtagataaa gtaattccaa tgttgccaaa aactctttca
     1261 aatggagtat gtagcttaaa tccatttgaa gataagttaa ctctttctat atttatggaa
     1321 atagaccata aaggaaatgt ggttaaacat gaaatatgtg aatctgtaat aaattcaaaa
     1381 gctagaatga cttatacaga agtatctgat atattagaaa aggatgatga aaaacttaaa
     1441 aagacttttg aacatgttgt tgaagatttt aaaaatgctg aaattttagc taggattctt
     1501 atgtcaagaa gggaaaaaag aggagctata gacttcaatt tcccagaggc aaagataatt
     1561 ctaaatggaa aaggcgaagt tgtagatata aagccatatg aaagaagaat atctaataag
     1621 ataatagaag aatttatgct tataagtaat gagacaattg ccgaacattt ttactggatg
     1681 ggaataccgt ttgtgtatag aatacatgag acaccatctt tggaaaaaat ggaagaattg
     1741 agtaagttta tatctacatt tggatatata ataaaaggtg ataaagagga agtacatcca
     1801 aaagcattac aaggtataat agagaaaata aagggcaaaa aagaagaagg agctatcagt
     1861 actataatgc ttcgttcttt aaaacaagct aagtactcac cagaatgtag tggacatttt
     1921 ggattagcag ctaaattcta ctgtcacttt acttcaccaa taagaagata tcctgacctt
     1981 caaattcata gaataataaa agaatcttta aataataaaa taagtggaaa aagacaagaa
     2041 cagcttacta ctatagtgga ttatgcatct gttcaatcat ccgagaaaga aagaaaggca
     2101 gagttagctg aaagagatgt acatgatttc tataaggctc tttatatgaa ggataaagtt
     2161 ggtcaagaat ttgagggtgt tgtatctagc gtaacttcat ttggaatatt tgttgagtta
     2221 gataatacta tagaagggtt gataagactt gcaaatatga atgatgatta ctatatttat
     2281 gatgagagtt cttatagtgt tttaggagaa agaactaaga agtcatataa gataggagac
     2341 cttgttaaga taaaggttga aagtgttaat gtagatttta aagaaattga ctttgagata
     2401 gttgaaaaaa tagaggagta gataaaacag ctaaaataag agataacaag ttgtagtatt
     2461 tatgatatgt ataaatatat agtgcacaat actctataaa ataaatgata aaaataggta
     2521 attgagaaat atctaatgga aatagttaac tgaaagataa ataattaaga aatatttatt
     2581 gaaagtagct aattgaaaaa taagtaattg agaaatgttt aatagaagtg gctaattgaa
     2641 aaataagtaa ttaagaaatg cttaatagaa gtggctaatt gaaaaataag taattaagaa
     2701 atgcttaata gaagtggcta attgaaaaat aagtaattga gaaatgctta atagaagtgg
     2761 ctaattgaaa agtaagtaat taagaaatgt ttaatagaag tggctaattg agaaataaat
     2821 aattaagaaa tgtttaatag aagtgactaa ttgaaaaata agtaattaag aaatgcttaa
     2881 tagaagtggc taattgaaaa ataagtaatt gagaaacatt taatagaaaa agctagctga
     2941 aagataagta attaagaaat atttaatgta aacagataat ttaaaaatac ctaattaaga
     3001 atagatatta aaaaatataa tttcaatata aaaataagtg tatcatttaa aatttaagat
     3061 acacttattt ttta
//
LOCUS       DAFKMX010000284         3042 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFKMX010000284 DAFKMX010000000
VERSION     DAFKMX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.291"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            103..1272
                     /gene="nth"
                     /locus_tag="LZB08_003598"
     CDS             103..1272
                     /gene="nth"
                     /locus_tag="LZB08_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBM7317058.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKKEMNKKGIDKKDIIEKEEINKKEMDKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            1466..1936
                     /gene="trmL"
                     /locus_tag="LZB08_003599"
     CDS             1466..1936
                     /gene="trmL"
                     /locus_tag="LZB08_003599"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBM7317059.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1972..2820
                     /locus_tag="LZB08_003600"
     CDS             1972..2820
                     /locus_tag="LZB08_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBM7317060.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         1287 a          266 c          584 g          905 t
ORIGIN      
        1 ttattttgta tgttttatat attgatgttt atttttttgt tgatatgatt aaaccattta
       61 taaatagcgt tttataaatt ggtattttga agggggataa ttatgaaaaa tgtaaatcta
      121 aaagagataa atgaaaaaga tgtaattttg gaagaagaga taaataaaaa aggaatggat
      181 aagaaaaata taattgagaa ggaagagata aataaaaaag gaatagataa gaaagatata
      241 attgagaaga aagagatgaa taaaaaagga atagataaga aagatataat tgagaaggaa
      301 gagataaata aaaaagaaat ggataagaaa gatataattg agaaggaaga gataaataaa
      361 aaaggaatga ataagaaaga tataattgag aaggaagaga taaatgaaaa aggaatagat
      421 aaaatgattg gtaaaaaaga tgagataatt gatgagaaag atatagagaa ttttatggat
      481 attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa gtactaaaaa aggtgaaacg
      541 gagaaaaaaa caaagaagag tagttctaat aaggctacac aaaaaacaag taaagaaact
      601 aaaagtaagt ctaaaattaa aaatgagtca gcaaatgaaa aagacgtaaa taagatttta
      661 gacgagttag aaaaattgta tccagatgct aaatgtgagt taaattatgg gacagccttt
      721 gaacttttaa tagcaactat tttatcagca caatgtactg atgtaagagt gaataaagtt
      781 acttcagagt tgtttaaaaa gtacaacaca gctagagact ttgctaattt gagcattgaa
      841 gaaattagta aagagataaa gagttgtggt ctttacaaaa gcaagtctca aaaaataaag
      901 gatacatctg aacagctatg tgaattatat gatggtgaag tacctgatag cttagaaaag
      961 cttataaaat tacctggtgt tggaagaaaa acagcagggg ttgtacttag taacgctttt
     1021 aatcatccag caatagctgt agatactcat gtatttagag ttagcaatag aataggaata
     1081 gtggacgagc caaatcctca aaaaacagaa tttgcactta tggaagctat accaaaagaa
     1141 agatggtcac actcacatca tgtattaatt tttcatggaa gaagaatgtg taaagctaga
     1201 aatccagaat gtgcaagttg cccaataaaa gaagattgta attattataa agaacttaat
     1261 gaaacgaaat aacttttaga aagtaagttg tacaagaggg attttctgat ataataatac
     1321 ttgtgaaaat gttagataaa aaactattat atacatgaaa taaaaattat tattaacatt
     1381 attttatata atagtataat attattgtta ttctgtttat aaaataaaat ttgataaata
     1441 aaagcgatac ttagggagga ttaaaatgtc attaaatata gtattagtag aaccagaaat
     1501 acctcaaaat acaggaaata taataagaac ctgtgcagca acaggttcaa ctttacattt
     1561 agtaaaacca ctgggttttt cattggatga taaacaccta aaaagatgtg ggcttgacta
     1621 ttgggatata gctgatatac aatattacga tagctttgaa gaattacaag aaaagtatcc
     1681 aaatagtaaa tacttttttt ctacaacaaa ggctaaacag tcacattcag atgtgaaata
     1741 tgaagaaaat tgttttatag tatttgggaa agaaacaaag ggattaccag aacctttgtt
     1801 gaaagcaaat atggatactt gtattagagt gcctatgttg gatgttgaaa aagctaggtc
     1861 attaaattta tcaaattcag ttgctattgt agtgtacgag gctttaagac aaataggata
     1921 cccaaatatg aggtagtttc aacaagccta aatgagaaag gagcatactt aatgaaaaat
     1981 ctaaagataa tctgtgacag tttatcggac gtgccaaaag atttgttaga taaatatgat
     2041 atagatatag tttcattaac agttatactg gaggaaagag aatacaagga tagaattgat
     2101 atatctggag atgaatttta taaaaggctt agagaagaaa atgtatatcc aaaaacttct
     2161 caagctactt atgcccaatt taaggaaatt tttgagaaat atacaaatga ggatagagag
     2221 atactttata tagcagggtc tgccacagct acaggtacgt atcaaagtgc tgtaatggca
     2281 aagaatgatg tagatggtaa aatatataca tatgactcta acttgctttg tatgggaact
     2341 ggtttacttg taataaaagc aggagaactt gcaagtgaag gaaagtctgt tgctgaaata
     2401 ttgcctgttt tggatgaatt aaaagagaag gcttttgtta tattttcagt agatacattg
     2461 gaatatttac aaaagggagg tagaatatca tctactaagg cagcaatagg aagtatatta
     2521 aatataaaac cgattcttga aataagagat ggattagttt cgcaaatatc tcaagtaaga
     2581 ggaaagaaga atgtaatttc taagatgatg gaattgataa aatcaaattg tggtgatgat
     2641 ttatcagacc aaattgtgta tataggatat agtgatgatt ttaaggaaaa ggaacaatta
     2701 tctaaggtta tgcaagatga attaagacct aaagaaatta gatttttcca aattggtgtg
     2761 tgtataggtg cacatgctgg tcctggtgtt actggtatca tatgcttaaa aaataaataa
     2821 tatatttttg ggtgacatta tttatttttt tattaaatta ggatagaata ttaattattg
     2881 agatgttaaa ttggatttat tttggatgaa attaagtgtt gatatattag atttagtttg
     2941 tagggaagtg tgtaaataga taagaatcta ttgcacactt ttttatagta agatttatta
     3001 aaaaaatttc aaaatgtact tgtaaattgt atataaaaaa at
//
LOCUS       DAFKMX010000285         3004 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFKMX010000285 DAFKMX010000000
VERSION     DAFKMX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.292"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(9..773)
                     /locus_tag="LZB08_003601"
     CDS             complement(9..773)
                     /locus_tag="LZB08_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBM7317061.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1047..1721
                     /gene="fsa"
                     /locus_tag="LZB08_003602"
     CDS             1047..1721
                     /gene="fsa"
                     /locus_tag="LZB08_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBM7317062.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1843..>3004)
                     /locus_tag="LZB08_003603"
     CDS             complement(1843..>3004)
                     /locus_tag="LZB08_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBM7317063.1"
                     /translation="TENFYIIILAYIITNAVTKTGVAKIISEKLILNTVNTPKKMILL
                     SYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITEDSKSILLFSIFTASTFTSMFF
                     VNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFITYFLFIFVFKKELHSIKFIS
                     NENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMFFMFLTQFIHNINTLIIMSIC
                     VLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKYSGVVDIMGNYLIKFIPNSSE
                     IISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHILKENSVTLCLFVYIIVSIQYI
                     LPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIIICITFPWWKFIGLEI"
BASE COUNT         1162 a          375 c          395 g         1072 t
ORIGIN      
        1 aattaaactt ataaaacatt tacattaaat tctgataaaa tatctactat atcaggagta
       61 ggcgtttttt ctgtaattat agaactaata tcttctaaat gagcaaactt gaaatttcca
      121 tcaaatttaa actttttatc ttccataact ataaatactt ttttacttga atttattata
      181 gccttcttcg taagaccatc ttcaatttca aaagttgtaa gtgaagagtt gaatacatct
      241 actccacaac ttcccataaa agtcttatca aacttatatt tcgatataaa ctctatagtt
      301 ggagtaccga caaaaccgtc taaactgaga tttaataaac ctccagtgga aattatattt
      361 aaattatttg gttttgaagt aactacattt agtatatcca gcatatttgt aacaagtgtt
      421 acttttttat tactttcagc taacaacttt gcaagcaaga tgttaattgt agagatatct
      481 aaaaatatag tttctctatc ttcaattaaa ttaaatgctt tttctgctat tattttcttt
      541 gttggaatat taatttcttt tctgtctttt gtatcctgat tctctagtgt ttctctagct
      601 agtacagctc ctccataaat tcttcttatt gaagaaatct tttctatatt ttttaaatct
      661 tttcttatac aatcttctgt aacattaaat ttagaactga gctctttgac agtaactcta
      721 ccttcttttt ttaggatatt taatatagct tggattcttt cttctgcaaa cattttctac
      781 ctcttttcgc aatatagtat tatagtaaat attataagat aaaatacaat atatcaacta
      841 ttttaacatg aaatacaaaa ttaatttaat ataaaaagta tgttttttct aactatatat
      901 ttagaattta gtaaataaaa aaattataaa actaagaagg aaaagtaaaa caataataaa
      961 aaataaaaaa caataagcaa aatgttgaaa ataataccaa atcaatatat aataagaata
     1021 aagaaagatt aaagggggat attttaatgc aactaatctt agatacaggt aatgtggagg
     1081 agattaagga gttatgtact tgtttaccaa tagatggtgt aactacaaat ccaagtattg
     1141 tttcaaaaga aaaaaagaat tttaagcaac ttataaatga aattggtgag attataggtg
     1201 aggatatgcc tatacatgct caagtgttaa gtacaaagta tgaagaaata ttagaagaag
     1261 cattatatat aagtagtttg agaaaaaata tatatgtaaa aatacctgta acacaagatg
     1321 gtttaagagc tataaaagac cttcatagaa aaggtgtaaa aataactgct actgcaatat
     1381 tcactgctca tcaaggtttt ttagctgcaa aggctggagc aaattatatt gcaccttatg
     1441 taaataggct tgataatata tctggtgatg gagtagctat ggtttcagaa cttataaaaa
     1501 taatagatac ttataaaatg gatacaaaag tacttgcagc cagttttaaa aatgcacaac
     1561 aagttataga attaatgcaa catggggtgc atagtgttac agtaccatat gatatttgta
     1621 aatctatgat gaatcatcca ttaactgatt ggagtgtaga taagtttata gaggattggg
     1681 agaatacttt tggaaaagga agtaaaacaa ataatattta gtattatggt aaaagagtag
     1741 ttttgtccaa ttttacaaga acttatataa aaagatgaaa atatgaattt tctttttaat
     1801 aatttcatta ttatcttaag ttaattcata ttttcatctt ttttagattt ctagtcctat
     1861 aaatttccac caagggaatg ttatacatat tattataaag taggttaaag gaatcaaagt
     1921 taaaccgtat ttgaaaataa ccttgctatt atacagattt tcaccatgac caaccattat
     1981 tgtagcatga tgaaatggta atatgtactg tatgcttact ataatgtaga caaatagaca
     2041 taaagtaaca ctattttcct ttagtatatg taaacttcca agtaatggta ttactacaga
     2101 tgatgtagtg acagcacttc caagacatat attcaagatt attgtgagag ttatcaaaaa
     2161 caatattgaa ataatttctg aagagtttgg tataaacttt atcaaataat ttcccattat
     2221 atctacaact ccagagtact ttaacactcc tccaattgaa aatgctgcta taaaaaaaat
     2281 aaggaccttc caatttattt cttttaaagt actaaatcca actatttttc ttagtaataa
     2341 aagaagtaca caaattgaca ttataattaa tgtgtttatg ttatgtatga actgagttaa
     2401 aaacataaaa aacattattc ctaggcataa aaatacgtgt ttttgctctt tgtttagcaa
     2461 attaaattct ttttttaaat tagaatatga aaaatgaagt ttattaccct caagattttc
     2521 attagaaata aattttattg aatgcaattc ttttttaaag acaaagataa ataaaaaata
     2581 agttataaaa caagtaatta tagttggaac agacatataa aaagcccatt gaccccaatt
     2641 tatattacaa tttccaagtt ctagtacaac ataatttaga agagtgtctc catttacaaa
     2701 aaacattgat gtaaaggtag atgctgtaaa tatactaaat aaaagtatag attttgaatc
     2761 ttcagttatc tgttgttctt ttaaaaactc cttgtaaaaa gcagatacaa gaataaccct
     2821 tggaaatggt tgtgggataa aaaatattaa aagtatacct aaaatatacg ataaaagaat
     2881 cattttttta ggggtattta cagtatttaa aattaacttc tcagaaataa tttttgcgac
     2941 acctgttttt gtaactgcat tagtaattat gtatgcaaga attattatgt aaaaattttc
     3001 tgta
//
LOCUS       DAFKMX010000286         2930 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFKMX010000286 DAFKMX010000000
VERSION     DAFKMX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.294"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(293..667)
                     /locus_tag="LZB08_003604"
     CDS             complement(293..667)
                     /locus_tag="LZB08_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7317064.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRALVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            886..1119
                     /locus_tag="LZB08_003605"
     CDS             886..1119
                     /locus_tag="LZB08_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317065.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1103..1516
                     /locus_tag="LZB08_003606"
     CDS             1103..1516
                     /locus_tag="LZB08_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317066.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            1535..1831
                     /locus_tag="LZB08_003607"
     CDS             1535..1831
                     /locus_tag="LZB08_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317067.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            2114..2431
                     /locus_tag="LZB08_003608"
     CDS             2114..2431
                     /locus_tag="LZB08_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317068.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
BASE COUNT         1066 a          363 c          381 g         1120 t
ORIGIN      
        1 ttgggtggaa gaatggtgga agaattacat ttcactaatt ttcttccacc attcttccac
       61 ccaaatatac ttagaaacat tcatttcact gcaataatag ctacttattt aaattctatt
      121 tttatacatt ctatctacta tatactgtac atattctatt aaaatttata taactaaaat
      181 acaaacttat ttcattttct taacatgttc agaataataa acaaaatgaa ggtagaaaaa
      241 gttataattt cataccttca ttttatttat tatgtatcaa tttgtcattt tcttatttca
      301 aattcttaaa atattcttcg agttcactta atttatcttc atccacttcg tcgtcttcac
      361 catgtaacgc tgacattaaa cttgaaattg aatttttatg tatgttcttc aaaaattcct
      421 ttgtttcaaa ccttagatat tttttctctt ttactagtat attataatgt gtaagtttgc
      481 ctattttctc tgtacttaaa aactcccttc gctctaatct tttcaaaact gtaaatgtag
      541 ttgattcatt ccactcatat ttttcttcca ttgcagcaac taatgcccta gaagttaata
      601 ccttgttagc ttcacaaata tatctcataa cttttaattc cgcttctggt aatttactaa
      661 ttttcataat tcgcgcccct ttgccgattt atgtatatgt taaattctat atcaataaag
      721 ttttacaaac aagagaatta aggtaaataa ttccatctaa tttttaccat attattcttt
      781 taggttttat aatacttatc taatgagatt gacattttac tcattttagg atattatgaa
      841 gttaacttca catgaagtta gcttcatata gaaagaggtt atggaatgaa aacaaaagta
      901 cgagaatttc gaacaaatat gggtctaaca cagcaacaac tcgctgattt agtccatgta
      961 tcaaacagaa caattatttc tattgaaaaa gagcaatata gcccttctct tatgcttgcc
     1021 tatcgtatgg cacaagtgtt tggtacaacg attgaagaac tatgttgttt aaaagaaaat
     1081 aaagaaatgg aggataaaaa acatgaaagt aaagagtaaa cgaagtttta tagtgggtat
     1141 tatagtttgt gtgttatgtt gtgctagttt agttatttat tgcattttaa aagataaaag
     1201 attcctaatt tcatcatttc ttttaattgt aatagcaatc tttaattttt gtaatgcttt
     1261 ttcaagaaaa ggcattgttg aagaattaca cgatagtaca gatgaaagag acttgtactt
     1321 aacaatgaaa acaagtcata tcttagttaa aattatgaat tatacattat ttacttttac
     1381 atttcttttc atcatagctt atagtgcatg gaaaaatcaa tcacttctag ttatagctat
     1441 aacactatgt gtcattgaga tttttttatt tgttgcatat ttacttatca atatattttt
     1501 agagaaaaaa gagtaaatac aaggggtgat ataaatgtat tttaaaactt atatactaaa
     1561 aagagaagta aataacaaac aactagagat aactcgaagt tttgacttag ataatctaat
     1621 ttgtactgca acagagattt tgaaagataa tgagattgat ttgataatta ctgatgaagt
     1681 aaataatgtt gtttggagaa atgaaaaaag cactccccca gcaagagaat gcttagttat
     1741 agctatagaa gtgtattggt ggtacacttc tattttttta ttatatcata taaattatgt
     1801 tatcaaaaag agctatttaa atcgatttta aggtgtgttg aataagattc ttgatagttt
     1861 atatattgat gaatttcaaa aaaataagca ctctttatta taaggagtgc tttggtgtat
     1921 gttaatcttc tatctaatac aattataaca tgtcttatgt tttagtatga taattttcgt
     1981 tcgctttatt attacaattt ttactatagt tttatctatc ctgttaatat ttgtaatatt
     2041 ttagatactc tttgtacttg tgttactgat atactttgat taagaaaagc aaatttaata
     2101 ggaggtatct gctatggatt ttggaaatat caatcttata cttattggaa taatagttat
     2161 aataggaact acaataatat atttaattaa acctaaaact gctttttgta gtaaaaaata
     2221 ttttaataaa ttagaatcaa tatatggaaa tatagataag aaaaaaactg ttaaactaga
     2281 agtactttat cgctatgtaa caggtcttga gtatatagca ataggcttat ttacaagaag
     2341 attagatatt acaatattag caataatatt agtagccatt ataacaacag cattatatta
     2401 tctaattaga aagaaatata taactatata aaaaataata tcttataaat aataagccta
     2461 tcattatact ctatttttag ctaaaattgt tcgacaaatt gctgatataa gttcttaaat
     2521 atgctataaa ttaaagtata ttatataaca ttgtaggagg gattttatag attttgggga
     2581 agttaaatca gagttaagtg acaaagacct taaagataat gaagaagctt taaacaagta
     2641 taaaaaatat tatttaaaag tttataagta ttatagtgaa atggattcca taaaaagtat
     2701 tcaagatata gaaatagagc aatctaggct taaaggacag ttacaaaaaa atagtaaccc
     2761 catttttgct ttgtatatat ctattatggc tgttgtattt tctacaacaa ttaacttaac
     2821 atttgattta tcagatatca ctaattattt tattagaatg attatgacac ttttatttat
     2881 tgtacttctt atttttttag ttaacaaggg cattcttcca atagctatac
//
LOCUS       DAFKMX010000287         2926 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFKMX010000287 DAFKMX010000000
VERSION     DAFKMX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.295"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            69..437
                     /locus_tag="LZB08_003609"
     CDS             69..437
                     /locus_tag="LZB08_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7317069.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            440..811
                     /locus_tag="LZB08_003610"
     CDS             440..811
                     /locus_tag="LZB08_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBM7317070.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(955..1410)
                     /locus_tag="LZB08_003611"
     CDS             complement(955..1410)
                     /locus_tag="LZB08_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317071.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1449..2714)
                     /locus_tag="LZB08_003612"
     CDS             complement(1449..2714)
                     /locus_tag="LZB08_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBM7317072.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1080 a          406 c          407 g         1033 t
ORIGIN      
        1 tggttgaaaa atctaattaa gagtactata atttaattaa gtaattactt aatcaaaacg
       61 aggtacttat ggaaaacttg gttaaaatat ttaaagcatt gagtgatgaa actagactta
      121 acatacttat attagtgtcc aagagaaata tatgtcaaaa ggggatttct aaatatctag
      181 ggatttcaga ttctgctgtt tcacaacata taaaaatatt aaaagatgtt gggataataa
      241 caggatataa agaaggatac tatgttttat atcatattaa taaagaatca tttaatatat
      301 gtgtagattt tataaattct atgttaagta attctagtga aagctttata gatgtatttg
      361 atgtaaatac aatacatcta ggatgcagta aagaatgtaa atctataaaa aaatgttgta
      421 agagaaggga gaaataataa tgaaagtatg tataccagta gaggagaata aaggattgga
      481 tagtaaacca tatggacatt ttggttctgc tccaattttt gttgtgtgtg atttagaaag
      541 tggagaagtt aaatctttag ataatggaga tttagatcat gaacatggaa aatgtcaacc
      601 attaaaagca ttgtcaggga cagctgtaga tgcagtagtt gttggtggaa taggacaagg
      661 agctataatt aaattaaatg gaatgggaat aaaagtttac agagcagaag gtgatactat
      721 aagtgctaat ttagacttac ttaaaaatgg aaaattagta gagtttccat cagaccatac
      781 ttgctcacat gacggatgtg gacatcatta aaaaaaacag ctgatgaaat atatacttca
      841 tcagctattt tttcttttaa taaatacgat tttattcttt taaaaccaaa atttagattt
      901 ttatgttttt tgtagtatcc ttaataatat ttcaagttta caagtttact acttttagat
      961 tttttttgta tgtttttcta tatagtcaat atcatttaat atgtcattga gttctttatt
     1021 aagtgttttt aaatcatcac catctacatt gtagtttaaa aatagttcct ctacattatc
     1081 aatgatagaa ttgacttttc tagtaacttc aacaaatgtt ttaaagttac tactattttt
     1141 agcatttaga aattttttta aatcctttga tataaatgat actatttcat attcatcatt
     1201 aaatagacct ctaccaatag gatatggttc aattctctgt aaaaaatatt ggtctttagc
     1261 attaatatta taagtataag atatttccca accatctcct aaatcaaact tgataaaata
     1321 taatgtacta cttacttttg atattcttgt ggcaccaagt tgtcttaaaa gaacttcaat
     1381 cgaatcacct tgaacagtgt taatattcat atatacacat cccctttagt attattatac
     1441 tatatttttt aaaattttat aattaaataa acagtagata taataataga tataatcata
     1501 agtggaaaac ctattttgaa atactcctta aaagagatag gatgaccgtg tttattacct
     1561 atacctgcta gtacaacatt agcagatgct cctattagtg ttccatttcc tccaagacaa
     1621 gctccaagag atgttgccca ccatagtggc attacatcaa taccttcagc ttgcatagtc
     1681 aaaataagag gaattagtgt tgcaacaaat ggaatattat ctaagaaaga agagactata
     1741 gctgataacc ataatatcaa caacattgta aatacaagat gtccttctgt aagtccaatc
     1801 aatgcctgag ccaatttgtt tattatccca acttctacta atccaccaac tactacaaat
     1861 aatcccataa agaataaaat tgtagaccat tcaatacctg ccataatttc atctacatct
     1921 tgttttccta ttaaaagcat tatagatgct cctgtcagtg ctacaacaga agaatctatg
     1981 tggattgtac tgtgaaacat aaaaccaaaa agtatgaatg ctattaatat aaggcttttt
     2041 ataagtagag gtttatcttt aacagatttt ttttcatcta gttttaatat tgcattttta
     2101 gctctttcat tcacaacaag ttcttttcca taaataaatc taaagcatat tatagttata
     2161 gcaagtatta ctataacagc aggacctaaa tttattacaa aatccataaa actaagattg
     2221 gcagcactac caatcataat atttggaggg tctcctataa gagtagctgt tcctcctata
     2281 tttgaagcta aaatctgtgt tattaaaaat ggaactggat taagtcctaa tatttgtgta
     2341 ataactatag tcattggtcc aataagtaag acggttgtaa cattatctaa tacagctgat
     2401 aaaatagctg ttataatagc aaagcaaatc attatcttcc atgggtcacc tttagctttc
     2461 tttgcagtcc agatagctat atattcaaat aaccccgagt ttttgacaac agctacaaat
     2521 agcatcatac caactaatac accaatagta ttgaaatcta tatggcttaa tccttcatca
     2581 agtgtaagta tattgaatat tatcattaat agtgcaccac caacagctgc tacggatctg
     2641 tttacctttt ctgtaataat agctgccata actattaaaa atatacatat agctattatt
     2701 tgttgaaatt ccataatata agtacctcca tttgtattat tttgtgctta atgctagtac
     2761 tatatatagt attacttttc tattgttttt tcaatggatg tatgaaagta tattatttaa
     2821 caaagttttt gaaaaattga cagaatttaa agaaaatttt tgttatttaa ataaatttta
     2881 aaattattcc attgataata atattaaacg acaaacgaaa aaatat
//
LOCUS       DAFKMX010000288         2921 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFKMX010000288 DAFKMX010000000
VERSION     DAFKMX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.296"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            68..141
                     /locus_tag="LZB08_003613"
     tRNA            68..141
                     /locus_tag="LZB08_003613"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="LZB08_003614"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="LZB08_003614"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="LZB08_003615"
     CDS             540..2558
                     /locus_tag="LZB08_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBM7317073.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1168 a          357 c          498 g          898 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg tacttattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgctcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaatacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggcc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact
     1801 taaaatctcc aatacttata acaccaaaga catatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcggcaaat aaggtatata aaataggtgg aggattgact tctaaggtaa
     1921 tgagctctat agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata
     1981 gtgggggcaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt agagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagacaca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga attttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatctg caattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aagtgcgaaa tgaggtatat
     2701 acagtctttt tatgattaat tgtaaaagga tttatattat atgaagaatt tacatatctt
     2761 aaggctacag ttaattgtta agagttggag tattcatttg taatacacaa tataagctta
     2821 attactatat tttattgtgt gtttgtgtgt tttttgtgga tttttatgtc aataaatgat
     2881 atagtataga taattaaatg tattaaatat atttaatttt t
//
LOCUS       DAFKMX010000289         2912 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFKMX010000289 DAFKMX010000000
VERSION     DAFKMX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.297"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..417
                     /locus_tag="LZB08_003616"
     CDS             <1..417
                     /locus_tag="LZB08_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7317074.1"
                     /translation="LSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLIYINKNRNCSL
                     NDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGEEVLGDIKNIF
                     SKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            419..1513
                     /locus_tag="LZB08_003617"
     CDS             419..1513
                     /locus_tag="LZB08_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBM7317075.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1601..2131
                     /locus_tag="LZB08_003618"
     CDS             1601..2131
                     /locus_tag="LZB08_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBM7317076.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASEKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT         1178 a          283 c          496 g          955 t
ORIGIN      
        1 ttgtcatata ttatttgtaa cttgcaaaaa aactttaaac tttattgtga aaaatgttta
       61 aaaccatata aacttacgaa tggtctatat ttctatttaa tttatattaa taaaaatagg
      121 aattgttctt tgaatgatgt ttctacagaa tttgaagttg ataaagctca tacaacaaga
      181 actatatcta ggttagaaca agatggatac atagaaaaaa tccaaaaccc taatgatagt
      241 agagcttttc agttgagagt aacagataag ggtgaggaag tattagggga tataaaaaac
      301 atattttcaa agtgggataa tcacataaaa aaagagttct cggatacaga atataaagaa
      361 ctggtaaaga atttacatgt ggtaaaggat ataaaaacag ctgtggagga agaataggat
      421 gtattgtaat ttattaaaac caattaaaat tggaaatttg gaattaaaaa atagagtatc
      481 ttttgcacct acaagcatgg gtcttaaatt ggaagaaaaa attaaaaagt tttctgatat
      541 tgctaagtca ggagtagctc tgattactct aggagatgta tctattagac ctagttttca
      601 taaggtagct ataagcctat cggatgaaga tggagtcatg aaatacaaga aaattgtaga
      661 tgaaatacac aattctggtg caaaggtatc agcacaacta ttttgttcag attatgatgt
      721 taatttaatt aaagatacta tgaagatggg aattacttca catgatgaga taaaaaaaat
      781 aatgaatgac ggagtaaaag attacataac aaatatgccc aaagaagaaa ttaaaaatat
      841 tataaattta tttaaagtaa cagcattgaa tgcaaaaaaa gcaggttttg acatgattca
      901 aattcatggt gataggcttg taggaagttt ttcatcaagt atatttaata atagaaatga
      961 tgaatatggt ggaacgtgtg ataatagaag tagatttgca agcgaaataa ttagtagtat
     1021 ccgagatgaa gttaaggaca ttccaataga ctataaattt gcaataagac aagaaaatcc
     1081 tcattatgga aatgcaggag ttttactttc ggaagttgag tattttgtta aaaaatttga
     1141 atccctaggt gtaaacagtt ttcatgtaac acttgcaaat cattcaaaat tagaagatac
     1201 tataccaacc aataatcatc cttactttaa agatgaaggc tgttttttat atttagctga
     1261 tgaggtaaag aaacacacaa atcttccagt atgtggggtt ggaaaattgt ctagcccaga
     1321 ttttatagaa agtattattt caaataatag agtagatatg gtttctatgt caagacaatt
     1381 actagcagat agtaattggt tacagaaagt aaaagatgga agagtagatg agattaaaaa
     1441 gtgctgttat tgtaataaga agtgtgcaga tgctttacaa acacgctctc agtttggatg
     1501 tatactagat tagaatatcc ttataaaaat aaataatttg ttaaggttaa tatatttttg
     1561 gtgatttaga taagtattta gataaagagg aggagacatc atgaaaagtt tagttttata
     1621 ttcaagttta actggaaata ctaaaaagat tgcttatact atatatgatg aaattcaaga
     1681 agaaaaagac attaaagatg taaatgaatt agttgattat agtattgact atgaaaatta
     1741 tgatatagta ttcttaggat attgggtgga caagggaatt tgtgataaaa attcaaaaca
     1801 agtgcttgaa aatattcaca ataaaaagat agcactattt ggaacaatgg gagcaagtga
     1861 aaaaggaagt tatggagcta gtataataga aaagatagaa tcaattatcc caaaagataa
     1921 tgaaatatta ggttcattca tatgtcaagg taagatagca gaaggattga aagcaaaata
     1981 taaagagatg ttaaaacttt ctcctgacaa cgaacatatt cgccaacaat taaataacca
     2041 tgaggaatct caaagtcatc cagatgaaca agaaatatat gaagccagta tgtttgcaaa
     2101 aaatatgatg attaaagcta gtattgtgta gtagttaaac aataagttca tataagatat
     2161 agaataaata atttagtaaa aaagtatagc aaataatttg ctatgctttt tttatgaaat
     2221 caaaaaaata ataaaaatac ttgactgtaa accttgttta tacattattt tatagaaaat
     2281 aagatagttt taaatataaa aaaagatata ttattgaaag gagatattat tgtatgaaaa
     2341 cttataaaag ataattagtt tgattttaag atatactaaa tatacaattt tatagagtga
     2401 aaatatttaa gactatatta tagttataaa ttaatagaat agagaggtag tgtttaaatt
     2461 gcaataacag tatttccaaa gttgccacct caaattccta tccaatggaa taatggtgta
     2521 gctaattatg tagataaaaa atttatttat gcctaccctg tcatttgtgt tgtattcaga
     2581 atccttttac gtccatttat atggagatgg ttacaaatac atgcttttta tagtgatact
     2641 attgcagatt atttaacgaa ttttatgtgt tttgtagcgt tgtctataga aatattctct
     2701 atacttttta tttacaatat tgccagacat gttacaataa ttatttttat agatatactt
     2761 gtatttattg ctttactgat aataggtgtg tataaattat ttaaaaaata caattaattt
     2821 ttaaaaataa gtaataagtg atagtgtaaa aagtagccac tgcaaattat ggattgtagt
     2881 ggctatttta ttatttgtat atgaaattat tt
//
LOCUS       DAFKMX010000290         2872 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFKMX010000290 DAFKMX010000000
VERSION     DAFKMX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.298"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            135..1421
                     /locus_tag="LZB08_003619"
     CDS             135..1421
                     /locus_tag="LZB08_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBM7317077.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGILYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            1507..2814
                     /gene="grdB"
                     /locus_tag="LZB08_003620"
     CDS             1507..2814
                     /gene="grdB"
                     /locus_tag="LZB08_003620"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2554..2556,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBM7317078.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         1110 a          318 c          612 g          832 t
ORIGIN      
        1 taaaaatgaa aatttctatg acattaatac atagaatata tattttaaat aattggcata
       61 gaaaatgctt tatatattaa taaacgtaat aaatactttt aattgcgatt aaatatatta
      121 agggggattt tcatatgaag ctagagctag ggaatatatt tataaaaaat gttgaatttg
      181 gagaaaagac agaagtaaaa gatggaatac tatatattag tagcgaagaa atagaaaaga
      241 tagtattaga agatgaaagg attgtaagtg taaatgttga acttgcaaga cctggcgaat
      301 caataagaat agctccagtt aaagatgtaa tagagcctag agttaaaatt ggagatgaaa
      361 gcaaaatatt tcctggaatt ataaataaag tgaagactgt aggaagtgga agaacccatg
      421 tacttttagg agcttgtgtt gtaacttgtg gaaatatagt tggatttcaa gaaggcgtaa
      481 ttgatatgag tggtcctact gctaaatata cacctttttc aaaaacaaat aatatatgta
      541 ttgttataaa agcaaaggat ggtatagata ctcatgatta tgaagaagca gctagaattg
      601 caggattaaa aataggtgca tatgtaggtg aggcaggaag agaagttgag ccagatgaag
      661 tcattgtata tgagacaaag ccattattaa aacaagtaaa agaatatcca gacctgccaa
      721 cagtagcata tgttcacatg ttacaatcac aaggactttt acatgacact tattactatg
      781 gggtggatgc taaacagata gtgcctacat ttatgtatcc aactgaaata atggatggag
      841 ctataatatc tggaaactgt gtagcaccat gtgataaggt tacaacattc catcatttaa
      901 ataatccagt tatacatgac ttatataaaa gacatggaaa agatttaaac tttataggag
      961 ttattttgac taatgagaac gtattcttag tcgataaaga gagaagctct gatatggtag
     1021 ctaaattaat agaattttta ggagttgatg gagttctagt gacagaagaa ggttatggga
     1081 atccagatac tgacctaatg atgaattgta gaaaatgtag tgaagttgga gcaaatgtag
     1141 ttttgataac ggatgaattt cctggaaaag atggcaagag tcaatctata gcagacgcta
     1201 ctaaggaagc agatgcagtt gtttcttgtg gtcaaggaaa tctagtggtt catttcccag
     1261 caatggaaaa aattatagga actttagatt atgttgaaat gatgataggt gggtataaag
     1321 gatgcttaaa tgaagacgga agcatggatg cagagctaca aattataata gcatcaacta
     1381 tagcaaatgg ctataatcat ttgacagcaa gatattatta aataagtatt tgaatataaa
     1441 gttctgttga tgtaatcatt ggtctaactg atgtaataac tataaagtga tttggaggat
     1501 taagagatga gtaaaatcag agtggttcat tatataaaca atttctttgc tggtataggt
     1561 ggagaggaaa aagcagatat tccgccagaa aaaagggctg gtgtagtagg acctggaata
     1621 gcatttcaga gtcaatttaa tgatgatgca gagataatct caactgtaat ttgcggagat
     1681 acgtattttg gtgaaaatat tgagagtgca actcaagaac ttttaaaaat gataaaagaa
     1741 gaaagccctg atttatttat tgcaggccca gcatttaatg caggtagata tggagtagct
     1801 tgtgggacta tttgtaaggc agttgaagaa gagttaaata ttcctgtaat tacaggcatg
     1861 tataaagaaa atccaggtgt agatatgttt aaattagatt tgcatatagt gtctacagga
     1921 aattcagcag caagtcttag aaaggtagtt ccaataatga caaaactagg tcttaaattg
     1981 gttagaggtg aggaaatagg acctcctgaa gaggaaggat atataatgag aggaataaga
     2041 aaaaatttct tccatgaact tagagggtct gaaagagcaa ttgatatgtt ggttaaaaag
     2101 atgaaagggg aaagctttga aactgagtat ccaatgcctg aatttgatag agttacacct
     2161 atgaaacctg ttaaagattt atctaagata aaacttgccc tagtaacttc tggagggata
     2221 gttccaattg ataatccaga caaaattgaa tcttcaagtg ctactaaata tggaatttac
     2281 gatttaacag atatggattc tatgtcaaat aaagaattta caactataca tggggggtat
     2341 gacagagcat atgtccttga aaatccaaat ttagttgtac cactagatgt agttagagag
     2401 cttgaaaaag aaggtgtaat tggagaatta gtagattatt ttataactac tacaggaaca
     2461 ggaacgagtg ttggcaattc taagagattt ggagaagagt tttctaagaa attgttagaa
     2521 gatgatgtag atgctgtaat tttaacttct acatgaggta cttgtactcg ttgtggagca
     2581 acgatggtaa aagagattga gagagctggt atagcagttg ttcatatatg tacagtagta
     2641 ccaatatcac ttacaatagg agctaataga attgttccag cagtagggat accatatcca
     2701 cttggaaatc ctatgttagg agaagaggaa agtaaaaaaa ttagaaagaa tatagtttta
     2761 aaagctttaa atgcactaac agaagatata gaagaacaaa cagtatttga ataataaaaa
     2821 tacaacacaa cactgaaata aaaaaagata tcaaaaaata attttttaaa at
//
LOCUS       DAFKMX010000291         2866 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFKMX010000291 DAFKMX010000000
VERSION     DAFKMX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.299"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..555
                     /locus_tag="LZB08_003621"
     CDS             <1..555
                     /locus_tag="LZB08_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBM7317079.1"
                     /translation="IVLKINTIFDENKLREQLKTVLCLREGNIDTTNSTNLLINNLLD
                     EDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFDKGLLFPHSV
                     NEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYEEVLKIGQDV
                     KLTNKISKCRTFLEFKKVLLKNLI"
     gene            624..983
                     /locus_tag="LZB08_003622"
     CDS             624..983
                     /locus_tag="LZB08_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317080.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            1018..1374
                     /locus_tag="LZB08_003623"
     CDS             1018..1374
                     /locus_tag="LZB08_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBM7317081.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            1411..2703
                     /locus_tag="LZB08_003624"
     CDS             1411..2703
                     /locus_tag="LZB08_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBM7317082.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         1148 a          250 c          557 g          911 t
ORIGIN      
        1 atagtattaa aaataaatac aatatttgat gaaaacaaac taagagaaca attaaaaaca
       61 gtattgtgcc taagagaagg gaatattgac actactaatt ctacaaattt actaataaat
      121 aatttgttag atgaagataa atttatgata ttaaatgaaa agactatact tgcttcatta
      181 gaaaaaatga tggaccatct tatggatttg ggatgcattg atgataagtt tagaaaaaat
      241 atagtcaata gagaagaaaa atctcccaca gtatttgata aaggtttatt atttcctcat
      301 tctgtaaatg aaaaatcaga taagttttta atggcagttg gaattttaga agaaccaata
      361 gaatatgcaa atagaagtat aaaaataatt cttatgacaa tgtttccatg tgagaataag
      421 atagactcag acttattagt aaaaatttat gaagaagttt taaagatagg ccaagatgta
      481 aaattaacga ataagataag caaatgtaga acttttttag aatttaaaaa ggtgttactt
      541 aaaaacctta tataactatt gtacagcatt tatagttgtt atacagattt aaaaataatg
      601 tgatttttgg aaggtgtata aatatgaaat tattagtgat tttatttgtt gcttatattt
      661 taaatactat atttataatg aagcagaata aattgtattt taaaacttta aatagagcta
      721 aaaaaaaggg tgatatagtg tctacaggca aaaagaaaag ctatttttct aaaggaagta
      781 tagccattat atcttcagat tcagatgggt ttataaagtt tggagaaatt ttaaaaggac
      841 gtacagtaat ggctaaattt aaagagattg aagaaataga aggattagat atatatagtg
      901 cagagcaaaa atttaaagat gaagagagta ttgtacaagc tataaaattt ataaaagaaa
      961 aaataaatgt ttcttttaat taactcaata taaatataaa ttatcaggag gaatgtcatg
     1021 aaatatgaag ttaagataag tgggataggc agtttagtta aagagttaat ggatgaaagc
     1081 aattgtctta ttatatatga tgaaaccatt aatgatgacg acttaaagga tatatctgta
     1141 attcattcaa tagcaacgtt aaaaagtgat gttgaaatag gagatacact tactataggg
     1201 aatagagatt attgtatagt ttcagttggt gatattgctc agaagactct tagagaaatt
     1261 gggcattgta caatcaaatt tgatggaaaa tgtgaagtaa atttaccagg agaaatacat
     1321 gtggaaatag gaaatccaga tataactatt ggagatttaa taactatttc ttaatttgaa
     1381 aataaaatta ttataacagg aggaattggt atgttagatt taaacagaaa acttgcaaaa
     1441 ttggaagagg aaggtaaaaa aataaaagta gctcttatag gagctgggca tatgggaaat
     1501 ggaatggtaa gccaaatggc aaatatgaaa ggtattgaag cttctattgt tgtagatata
     1561 aatttggagt tggctcataa agcttttact gatgcaggaa tagatgaaga aattatagat
     1621 aatgtaacaa atgaaaagga tgcagaaata aaattacaag aaggaaaagt attgacatgt
     1681 aatgattttc ttgtggcttg taaaactaag tcaatagatg tagttataga tgcaactgga
     1741 ggtattgcaa ttggtgcaga gatagcatta aactctattt taaataaaaa acatatagtt
     1801 atgttaaatg ctgaaactga ttgtgttgtt ggaccaatac ttaaaaaatt agcagatgat
     1861 gctggagtaa tatttacagg ttctgcagga gatgagccag gtgcagtaat ggaattattt
     1921 gattttgcag atgcaatggg gtttgaagtt agagtgatag gtaaaggtaa aaataataaa
     1981 ttagatttag attgtaatcc tgatacagtt agagaagaag cagaaagaaa aggagcttca
     2041 cctcatatga tagcttcttt taaagaagga actaaaacaa tggtagaaat ggctttaatg
     2101 tgtaatgcaa ctggatttgt gcctgatgta agaggtggtc atggtattga agctacagtt
     2161 aatgaagtac ctaagaaata tgcattaaaa agcgaaggtg gagtattaga taattatggt
     2221 gtagttgatt ttgtaaatgg tatagcacct ggagttttcg tagtagtagc ccataaatta
     2281 aaagcagtta atgatgaact aaaatattta agtatgggtg atggacctaa ttacatatta
     2341 tatagaccat atcatttatg tagtttagag actcctttat cagcagctat ggcagtctta
     2401 gaccataagg ctacaatagt acctaaagct ggactagtag cagaagttat gactatagct
     2461 aagaaagatt tgaaaaaagg tgagtttatg gatggctatg gagcatatac atgctatggt
     2521 actatagaaa aatacgatgt agcaaaggct atgaatgctg tacctattgg tttgataagt
     2581 aaaaagacta aagttgtaaa agatattaaa aaaggtgaag ttataactta cgatatggta
     2641 gagatagaaa aagatactac tttatatcat ttaagacaat tgcaagaaaa aatatttggg
     2701 taagatttga tggtaatata aatagatatt agtaaaaagg gttgatggtt atttttaaat
     2761 caaccctttt aaatttttat atattcttgt taggatactt tctttaaaag tatattatag
     2821 gttatttgta gtcgtaaaaa ttattttgaa ttagttctgc aatttt
//
LOCUS       DAFKMX010000292         2826 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFKMX010000292 DAFKMX010000000
VERSION     DAFKMX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.300"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            352..1113
                     /locus_tag="LZB08_003625"
     CDS             352..1113
                     /locus_tag="LZB08_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317083.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1116..1934
                     /locus_tag="LZB08_003626"
     CDS             1116..1934
                     /locus_tag="LZB08_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBM7317084.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     IFNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2114..2716
                     /locus_tag="LZB08_003627"
     CDS             2114..2716
                     /locus_tag="LZB08_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBM7317085.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         1205 a          235 c          478 g          908 t
ORIGIN      
        1 atttgtgtta acagtatgga atatactgct taagtttaga ttggtgataa aaggatatat
       61 ttattaaatt tagtctaact gtacaaaata tattgattga gtttaggtta gcaataaaaa
      121 gatatattta ttaaatttgt gctaactgta cggaatatac tgcttaagtt tagattagtg
      181 ataaaaagat atatttatta aatttgaacc aactgtacaa agctatacta attaagctta
      241 gaataactat ataaaattat aacaataagt gtgtgggcag aaaaattctg ctacacactt
      301 tatttatatt cacataaaaa atttatcaaa aaaaagaaaa aggagtaaaa aatggaattt
      361 acaagagaac catttatact agatgacgat ttgtgtctag ctttggtgga caaaagaatt
      421 agttatctaa tggaaaatga attaaaatta agaaatatag agataataaa aacaatagaa
      481 tgtaaagaat tatatgaaag cataaaatat cacccggata tatgtatttg taatttagga
      541 aaaggagata taattgttgc gccgaatgta tataatcaat acaaagagtt attaagtaga
      601 tataaattta atataattaa gggagaagct aatttattaa atagataccc ttatgatata
      661 ccttacaata ttgcgatagt tggtgactat gctatacaca attttaagta tactgacaaa
      721 gcaatattag attatattga aaataataaa cttaaaaaga taaatataga gcagggctat
      781 tctaaatgct caatctgtat agtagataga acgtctataa taacatcaga taaaggtatt
      841 tggaaaagta tgaagaatac agaaatagag tgtttattga tagaaaaagg tcacataaat
      901 ttatttgaga tgaattatgg atttataggt ggatgtacag ggttgatttc taaggataaa
      961 ctagcttttt gtggagatgt taaaaaacat cctgactatg aacgtataaa aagttttgta
     1021 gaaagtaaaa acaaggagat agttacactt agctgtgaaa atttactaga tataggttct
     1081 atagttcctc tcatgacaag aaaggagcga taactttggt tgagaaggat aatatttctt
     1141 ccatgaaatt tttgaggaat aataatgtaa acgatagatt aatatatacg aatgacattg
     1201 tagaagtagt gatagattct gcaaagcaag aagtgttaaa taaaaaaata tttaatggta
     1261 ctaaagctga acaaagagct ttaaatattg atgagatatc aacggaaata actagtatga
     1321 taataagtat aatgaaagag aaacttttga aagaatacat tctaaaatgc tatgggcgta
     1381 catatcctga agataaaaat aatatataca tatattcact gaacatattt gctaaaaaag
     1441 agatttttat gagagaaaca gtatttacta gggtgagaaa ttatatagaa gaaaatgatt
     1501 ttataaacgt agaaggtttt attagattta gaatgagaga atttatgaaa tatatttctg
     1561 ctataggtga tattgcagtt gaggagtatt taataaagaa agaccaagat gaatttataa
     1621 gagttttaaa atactttata gatactcaag aagaaaagat tgatttgctt aaagttcata
     1681 taatggaaga taatacattt gttttatacg ataaaaatgg aaataagata gacagtatag
     1741 atgatgaaga aattataaat atggtaataa gagaaaattt aaattatgag gactttttaa
     1801 taagtacctt attaactcta tgtcccaaaa aaatagaaat attggatttg ttaaataata
     1861 attgctcaaa ggaaattata gatactgtag aagcaatttt tgaaaataaa gttagtataa
     1921 ttatggaaaa ctagaatcta agcatataaa agctatccat ataaaattaa aaaaattctt
     1981 tttaaatcga cacaaaagac ttataattgt atataaagct atagtatgac tagagttatt
     2041 aagtataaag aatatctact tgattaatac aatatagctg gcaaatatac aattgataag
     2101 gagtaatttt ataatggata atttaattag gatactgata ttagctgtta ttggtggatt
     2161 tataggttat gttacgaatg tagttgctat aagactgata tttagaccaa tagagcctat
     2221 taaaatacct attttaaata tagaaatagt aggtcttata ccaaagagaa gagcagaaat
     2281 agcagcaaat gtaggagaaa taattcaaga agaatttctt tctatggacg aaatacttgc
     2341 aaatatcatt actgacgaag ataaggaaga agttgtaaga tacataaaag caagggtaaa
     2401 aattataata catgagaaag tttcatttat cccgagtggt ataaaaaaca tgatacaaga
     2461 ttatctagga gagataattg agtctgaagt taagcaaagt atagatgaat tgagtaaaaa
     2521 tataataaat aaagcaaatg aacgtataga tatacaaaaa atggtcgaag ataagattaa
     2581 tgaactagat ttatatgaat tggaagagat aataataaga atagctaaga aagaattaaa
     2641 acatatagag tttttggggc ttgtcttagg gtttttaata ggtatagctc aagggttaat
     2701 tactatgttt atttaaatat atgagaagta gcatttattt aagtactaat tatatttacc
     2761 tatttgagtg aattacttat taataacatt tagatattaa ttgtatttac ctatttgagt
     2821 aaatta
//
LOCUS       DAFKMX010000293         2822 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFKMX010000293 DAFKMX010000000
VERSION     DAFKMX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.301"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            115..813
                     /locus_tag="LZB08_003628"
     CDS             115..813
                     /locus_tag="LZB08_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBM7317086.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
     gene            1626..2093
                     /locus_tag="LZB08_003629"
     CDS             1626..2093
                     /locus_tag="LZB08_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBM7317087.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            2104..2319
                     /locus_tag="LZB08_003630"
     CDS             2104..2319
                     /locus_tag="LZB08_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317088.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT         1074 a          321 c          337 g         1090 t
ORIGIN      
        1 aattagtttt aatactaata taattagtat tatattaaat tctatattac aacattattt
       61 tatagtttga ttaatttaac aatcactaat atatcaaact gaaaggagat ttttatggaa
      121 aaaaaatatt tcgaaattca tggagttcct tcaatccttt ggggaaatcc ttcaagtaaa
      181 ttatatattt acatacatgg acaatgtggc aataaagagg aggctgaagc ttttgctaat
      241 attgcagttc cacatgagtg tcaagtttta agtgttgatt tgcctgaaca tggagaacgt
      301 aaaagagaaa aaaattcatt taacccatgg catgttgttc cagaattagt atctattatg
      361 gaatatgcaa agtgccactg gaatcaaatt tcgttatatg caaatagcat tggagcatgg
      421 tttagtatgt taagctttga aaaagagaac ttaagagagt gtatttttgt atctccaatt
      481 ctagatatgc aacagctcat ttcaaatatg atgctttggg caaatgtatc agaagaacag
      541 cttaaacatg aactaattat cccaacttca tttgggcata ctctttcatg ggaatatcta
      601 caatatgtaa aacaacatcc tatcactcaa tggaatgtac caacaaaaat attatatggt
      661 aaacatgatg aacttactga aatcggtgtt gtagagaaat ttgtagaaaa gtttaattgc
      721 aaccttacta ttctggaaaa tggcaaacat tggtttaaca caccacaaga attaaattca
      781 ttatatgaat ggttaaatac tttagttaaa taaaaaataa ttatattatt aatatgatta
      841 aattgtttat aaatgttatt taattaattt tattttagca ttattaaaat aactacataa
      901 agaataaatt agttattatt aaagaaatta aaagtatata gagtattaat aattttttaa
      961 taattagtta ttatagaaaa aagagggata taatattgtc tccctctctt tcttctgtaa
     1021 tattatataa tttttattta tcactctcaa taaattatag ttatctaaaa aattcaatct
     1081 ttaatacact attaatatat tgtgatatta atattacagt attaaataaa atctaataaa
     1141 attaactata aaatatctat tatataaata tccactctta tgtttgaaat ccgtcatata
     1201 gttttatcct tcttatcttt tatatcaata acttttttta ataaaattta caaaactaaa
     1261 gtaaaaaacc ttattgataa tgaaacaaat gatactataa gttctaatat tatagtaatt
     1321 gcatataaat agacatcata aaaataatat aatattgtca ctataaaatt aaatgtaaaa
     1381 atctcaaatc taactaaata cacatatagg tattttttaa gtttttaggt atttcttctg
     1441 aaaatgattt attgattgat accaatttac ataatctttt ttatataaat ttaccacctt
     1501 atacattctt atttttagtg tatattacaa tggttttata tattatatca ataatttatt
     1561 atttaaattc aataaattat atattataat caataatata ctttatataa aggagatgat
     1621 attttatgat tacaaaattt tttagtaata tattatcagt atttctatat tttctattat
     1681 cacttttagg agttgctggt ataatattct tatgtatgat acctatgttt ttatatgatt
     1741 ttagttctga caaagtaatc gctttaattg caagaatact atatttttct gactacttta
     1801 tagtcatctt attcttgata tttataatta atagtactaa gtattcccca ttcgtacttg
     1861 aaaatgtaaa aaggtttaag gtaatgggat gttgtttatt gataaacact ataattgaat
     1921 gtgtaattgg ttataactca aatactagtc ttataatgat atttggagat aatagtggtg
     1981 gtatctcacc acttatggtt atttctttta tatttgcact aatgtgcttt gtaattgcag
     2041 aaacatttga taaagctata aaaataaaag aagataatga tttaacgata tagaggtgat
     2101 gttatggcaa tagtagttaa cctagatgta atgatggcaa aaaggaaaat atcacttaaa
     2161 gatttagcag aaaaaataga tattacaaat gcaaatttat caattttaaa aactggaaaa
     2221 gcaaaagcta ttagatttac cactctaaac gaaatttgca aagctttaga ctgtcagcca
     2281 ggtgatatac ttgaatatgt agaagatgaa aatgattaaa aaagagtgtt tagaaacact
     2341 cttttttatt atactatatg atttttattc ttttactata ttattgacat taatattaac
     2401 tttgtttaaa gtcataacaa aatatgttct aagaaagaaa ctgttccttt atctatttgt
     2461 tatccattac ctataagaac tgtatcacac taaataatac ttttaaattg ataattttac
     2521 cacagattcc tatatacatt cttttctacc taatatttat ttaaactgtt agcaaaacat
     2581 ggaacttttt catttactta tacccccatt tatattattg catagtttat agctacgtgt
     2641 tataaattac attattgatg attttatcta acatttgtaa tcttttagat actctttgaa
     2701 gttacaacac tgttatactc taattaagta aactaaatct gatacaagtt ggtgattata
     2761 tgaaaagaaa actttcaacc tattttaaag gatatatttt aggtttatgt atagcatttt
     2821 ta
//
LOCUS       DAFKMX010000294         2799 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFKMX010000294 DAFKMX010000000
VERSION     DAFKMX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.302"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1705
                     /locus_tag="LZB08_003631"
     CDS             <1..1705
                     /locus_tag="LZB08_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBM7317089.1"
                     /translation="PSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIIL
                     AFYAFFEFTSVIICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPL
                     VNTVLISGFIGKIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNT
                     ENFKRARKESVNLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDME
                     FLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSIS
                     KIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEE
                     ASHAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYY
                     GKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFE
                     NQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVE
                     KIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYL
                     F"
     gene            2027..2611
                     /locus_tag="LZB08_003632"
     CDS             2027..2611
                     /locus_tag="LZB08_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBM7317090.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1093 a          272 c          431 g         1003 t
ORIGIN      
        1 gccatctatt agatatatat ttgatatttt aataaaaagt ttacatatag tctacctaaa
       61 tatggataat tttataaaag ttttgaccaa tcccatgtca atagttctga taatactatc
      121 agttataata ctagcttttt atgcattttt tgaatttacc tctgtaatta tttgttttaa
      181 taaatcgata aaatatgaaa aaatagggtt atttgaactc tttaaaatat catttaagaa
      241 ctctataaaa ttgttatatc ctaaaaatat actattattt atatttgtga tattaataat
      301 tccattagta aatacagttt taatttcagg gtttatagga aaaatacaaa taccagagta
      361 catactagat tatataaagt ctgatataat tttaaatata atctatatga tttttctttt
      421 aataatatat atttcggtta ttagatggat ttttagcatt catgagataa ctttaaatac
      481 agaaaatttt aaaagagcaa gaaaagaaag tgttaatctt ataaagggaa agattataag
      541 aacaattatt tattcattaa tattatcttt agtagtagct attattggat atgcgattta
      601 tcatagtgga attatattta taggcatatg gactaaatat tgtactgata tggaattttt
      661 gaagaatgta tttataaata gatgtattgc atttgaagaa tattttagat tgattttatc
      721 aatattcatt tttatattgg acattggatt tatctcagca acttattatc aatataaagg
      781 agtaagtatt tctaaaaata atattaaaaa agagaaaaaa tctatatcaa aaattgtttt
      841 taatttagta ttaatattat taatttctta tatagagtca gttgcatttt cttcacatat
      901 aaatggtatg tttaatacgt cattttcatt ttatacaaca gcaattgctc atagagcagg
      961 agctgaaatg gctccagaga atactttggc agcaatagaa gaagcttcac atgctaaagc
     1021 tgattatgca gaaatagatg ttcaagaaac taaagatgga gaacttattg tacttcatga
     1081 ttcaaacttt aagagaacta caggagtaga taaaaatgtt tgggatgtaa attataatga
     1141 agtaaagaca tatgatgcgg gaagctttta ttattatgga aaaggttatt atgatgaaaa
     1201 gtttgttggg gaaaagatac caacactcaa agaaatgata aaatattcta aaggtaaggt
     1261 gaaattatta atagaaataa aacttaatgg acatgaaaag ggagacgtag taaaaaaagt
     1321 tgttcagtta attaaagaaa ataattttga aaatcaatgt atagttgcat ctatggataa
     1381 aactatatta caaaaagtaa aaaaactgga tgagaattta ctgacatgct attttatggc
     1441 aattgcatat ggtgattttt caaagttaaa ttatgttgat atatatggaa ttgaatcaac
     1501 ttttgttaat aaaacagtag tagaaaagat tcataaaatg aacaaaaaaa tcttcgtttg
     1561 gactgttaat agtgacagtt taattgagaa aatgcttgac ctaaatgtag atagtattat
     1621 tacagataat ccatacctta tagagagtgc aatttattgg aagaaaaatg gatttattag
     1681 tcaagtgtca gattatttat tttaaatatt ctataattgt attatccagt aaaagagata
     1741 ttattaaaat caataatatc tctttttaac ataaataagg ttgttaaatt aaggttattc
     1801 ttataaaaat tgtgcttatc agctataaac aaggaataaa agtgaaaaaa ttgctctgag
     1861 attattacag ttaaaagagt tttaagatga cagagagata gattgtcaac tgtatattta
     1921 ataaatcttg ttatttatta aattttatat aagtaaaata tataaattaa taaatcttat
     1981 gctagaatat atatgaaaaa caatataaat ttggaggatt aaaaacatga cagagcgaga
     2041 aaaaatgttg gcaggagaac tttacgattg tggtgatatt gagttgttaa ctcaatggca
     2101 taaagcaaaa gacctagtaa gagattataa ccaaacagat tcctcggatt tggaaaaaaa
     2161 agaaaaaata ttaaatgagt tacttggtgg taagggtaaa aacttatgga ttactgcacc
     2221 gtattatgta gattatggaa ataatattta ttttggaaat aattgtgaag tcaatatgaa
     2281 ttgtacgttt ttagatgata ataaaattat cattggggat aatgcactaa tcgcaccaaa
     2341 cgtacaaata tatactgcat ttcatccaac taatgctggt gatagatttg gtaatgccaa
     2401 agaagatggt tcatttgaat tttgtaaaac tcaaacagct ccagttacta ttggagataa
     2461 tgtttggatt ggcgggggtg ctattatcat gccaggtgtt actattggag ataatgtagt
     2521 tataggtgca ggaagtattg ttacaaaaga tattccaagt aatatgattg catacggaaa
     2581 tccatgccgt attattcgag aaaataaata aagttaaatt ttcttgcaaa atctcaaaaa
     2641 atacctttct tactttatac atagggtatt ttctaatact ttcacatcca tattcatttg
     2701 ttaaattttt gtggaatgta gtttttatca cttctatctt acatttccac cattttttta
     2761 taaaagtcat tcctttatag atttatttat aatagtata
//
LOCUS       DAFKMX010000295         2796 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFKMX010000295 DAFKMX010000000
VERSION     DAFKMX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.303"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..217
                     /locus_tag="LZB08_003633"
     CDS             <1..217
                     /locus_tag="LZB08_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317091.1"
                     /translation="SFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDFFNSDGTFNLTL
                     SFDIQKNENLLDSLLELRKSIIINERI"
     gene            254..>2796
                     /gene="clpB"
                     /locus_tag="LZB08_003634"
     CDS             254..>2796
                     /gene="clpB"
                     /locus_tag="LZB08_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBM7317092.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNG"
BASE COUNT         1170 a          289 c          545 g          792 t
ORIGIN      
        1 gagttttata gataaatgtc agtatttatt aataggtgaa aaaagactta tagatgaaga
       61 cttgtattta atagcaaaat caaaagaagt attcgatttt tttaattcag atggaacatt
      121 taatttaaca ttgagtttcg atatacaaaa aaacgagaat ttacttgata gtcttttaga
      181 gcttagaaaa tcaataatta taaatgagag aatttaattt atttggatat aatattaaaa
      241 tggagggatt tatatggacg tagaaaaaat gactttaaga gttcaaaaaa gtttaaatga
      301 ggcttataac gaagcagtaa aaaatcataa ccaacaagta gatgtaattc atcttttctc
      361 agcacttatt aatcaagagg atggattgat accaaatata atcgaaaaaa tgaatatatc
      421 aattgattct ttgagaaata ctgtaaactt tgaaattgat aagcttccaa aagtatatgg
      481 agaaggtgct gactcacaag gagtatcagc tactagaaaa ataaatgagg tgcttataaa
      541 ggcagagagt atttctaaag aatttaaaga ttcatatata agtgtagagc atgttatgct
      601 tgcaatgatg gaaacagaat caaaatcagc tgttggtaaa atattaaaac aatataatat
      661 aaataagaat gactttttaa atattttatc acaagtgaga ggtagtcaaa gagtagaaac
      721 acaagaccca gaaggcacat atgatgcact tgctagatat ggaacaaact tagtagattt
      781 agccaagaaa aataaattag accctgtaat tgggagagat gaagaaataa gaaggattat
      841 aagaatttta tcaagaagaa caaaaaataa tcctgtattg attggtgagc caggtgtagg
      901 taaaactgca attgtagaag ggttagcaga gagaatagta agaggagacg tcccagaagg
      961 tttaaaagat aagattgtat tctcacttga tatgggagct ttaattgctg gtgcaaaata
     1021 tagaggtgaa tttgaagaaa gattaaaggc agttttaaaa gaagttcaaa gttctgatgg
     1081 aaaaattata ctgtttatag atgagattca tactattgtt ggagctggta aaacagaagg
     1141 ttcaatggat gcaggtaatc ttattaaacc tatgcttgca agaggtgaat tgaactgtat
     1201 tggtgcaact acttttgatg aatatagaaa gtatatagaa aaagataagg ctcttgaaag
     1261 acgttttcag cctgtaattg cagaagaacc tactgttgaa gatacaatat ctatacttag
     1321 aggtttaaaa gaaagatttg agatacatca tggagtaaga atacatgata atgctattgt
     1381 agcagcagca aaactttctc atagatatat acaagataga tttttaccag ataaagcaat
     1441 tgatttaatt gatgaagctg gagctatgat tcgtagtgaa atagattcat tgccaacaga
     1501 attagatgtt gttagaagaa agttatttac attagaaact gaaagagaag cattacttaa
     1561 agaaaatgat gataaaagta aatcaagatt ggatgatatt caaaaagaaa tagcagagtt
     1621 aaaatctaaa aatgatgaaa tgacagcaaa atatgaaaaa gaaaagagtc aaatattaga
     1681 tattaaaaac ttaaaggctc aattagacga agctaaaggt aaagctgaaa agtatgaaag
     1741 agaatatgat ttcaacaaag cagcagaggt taaatatggt gaaataccta agttagaaga
     1801 acaaataaag cagtacgaag aaaatatgaa tgatggttct gaaaattctc ttttaaaaga
     1861 agaagttact gaagaagaaa tatctagcat tgtatcaaaa tggacaggta taccagtaac
     1921 taaactagtt gaaggagaaa gagaaaaatt acttaaacta gaagatgaac ttcataaaag
     1981 agttattgga caagatgaag cagttacagc agtttcaaat gcagtaatac gtgcaagagc
     2041 aggattaaaa gatgaaagaa aaccaattgg ttcatttatt ttcttaggcc ctacaggtgt
     2101 tggtaaaact gaacttgcaa aaactcttgc tagaaattta ttcgatagtg aagataatat
     2161 aataagaata gatatgtctg aatacatgga aaaacatgct gtatcaagac tagttggacc
     2221 tcctccaggg tacgtgggat atgaagaggg tggtcaactt acagaagcag ttagacgtgc
     2281 tccttattct gtaatactat ttgatgagat agaaaaagca catgaggatg tatttaacat
     2341 gttcttacag atattagatg atggtagact tactgataat aaaggtaaga cagtagactt
     2401 taaaaatact cttataataa tgacttctaa tataggaagt aattatctat tagaagcagg
     2461 aggaaatata acagagacta ctaacaattt agtaatgaat gaaatgaagc atagatttaa
     2521 acctgagttt ttaaacagag tagatgatat tataatgttt aagcctttag accaagaaaa
     2581 tatcaagaaa atcatagata tatttatgaa agatttaaaa aatagattaa aagaaaaaga
     2641 tattactatt gaagttacaa attcagcaaa agatgttatg gtgagagaag gatatgaccc
     2701 tgtatatgga gcaagacctc ttaagagata tataggaaat acattagaaa ctataattgc
     2761 aaagaaactt attgctggag atgtgtataa tggatg
//
LOCUS       DAFKMX010000296         2773 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFKMX010000296 DAFKMX010000000
VERSION     DAFKMX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.304"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            173..1096
                     /locus_tag="LZB08_003635"
     CDS             173..1096
                     /locus_tag="LZB08_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBM7317093.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1071..1898
                     /gene="panB"
                     /locus_tag="LZB08_003636"
     CDS             1071..1898
                     /gene="panB"
                     /locus_tag="LZB08_003636"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBM7317094.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1917..2765
                     /locus_tag="LZB08_003637"
     CDS             1917..2765
                     /locus_tag="LZB08_003637"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBM7317095.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PDNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1099 a          329 c          460 g          885 t
ORIGIN      
        1 tgtcaatttt ttatgaaaat ggtaagatat ttatggatgg tatcttgtta taagaactat
       61 actttaaatg tttttaatta ttttgtctta tgtaactcat atcctcagga atgagattag
      121 aaattaagat gttgtatcac aaagtggagg tgatactatt aaaataggct ttattggttc
      181 tggaaaagtt ggaacatctt tggcaaatta ttttttatca aaaggttatt gtgtttcagg
      241 cttttatgga aggaatcaat tatctttatt agaatcaaca aatttaacaa aaacaaagat
      301 ttacagcaat ttgaaagata taatttatga aaacgatata ttatttatta caacatcaga
      361 tgattctatt gaaattatag acaaaaaact ttccaaattt aatttaaaac ataaatacat
      421 ttgccacaca agtggctctt taaaatctag tatattgttc tgttcaaaaa aagcaggtgc
      481 attaatttat tctatccatc ctatatttgc attttccaat aaaaatacca acataaaaaa
      541 aatgaaagac atttgttttt ccatagaagg tgataatgaa caagatgatt tagttattca
      601 aaatttcata gatgatttgg gaaaccaatt ttttataaga gataaggata cttcatccac
      661 ttatcattta gctaatgttt tagtttcaaa tttagtatta tctttactag atataggagt
      721 aagttacttt attaaattag gtttatctga agaggaatct ttaaaagcta ttagtccttt
      781 agtaaaaaaa aatatagaaa atatacttga taatggcttt actaaagctc ttactggtcc
      841 tgtttctcgt ggagatataa caccaataag gaaacatcta tctgtattgg ataaaaaaga
      901 tgaggatatt tacaaaatac tatccttaaa cctactaaaa ataattgcct taggaaatga
      961 caattcatta aaaggtataa aaaatattac tatagaaaat gcaatcgaaa atttaataag
     1021 taaatctgaa aaatacaaag aaatttacaa acttttagga ggaaaaaacg atgaaaaata
     1081 ccatacagac ttttaagaat gctaaatatg aaggaaaaaa actatctatg cttactgctt
     1141 atgactactc aattgcaaaa ataatggatg agtgtgatat aaatggtatt ttaataggag
     1201 attcattagg aatggttata aaaggtgaag aaaacactct atctgttacc atagatgaaa
     1261 ttatatatca tacaaaagct gtaaaaaatg gtgtaaaaaa tgccttaatc gtaagtgata
     1321 tgccttttct atcttaccat gtttctatag aagatgcagt taaaaacgct ggtagactta
     1381 taaaagaagg aggagctcat gctgtaaagt tagaaggtgg ctcaaacgtt ataaagcaaa
     1441 tagaaagcat tgtaaatgct caaatacctg taatggggca tttaggatta actcctcaat
     1501 cagtaaattc atttggtggg tttaaggttc aaggaaatac cagtgaaaca gcaagacaac
     1561 ttattgaaga tgctaaatta atagaaaaag caggtgcatt ctctattgtc cttgaaggag
     1621 tgcctacgaa aatagctgag atggtaacta attctatttc aattcctaca ataggtatag
     1681 gggcaggaat taactgtgat ggacaaattt tagtttatca agatatgttg ggaatgtttg
     1741 gagattttgt tcctaagttt gtaaagcagt atgcaaatat aggagatata atgaaagatt
     1801 caattaaaaa ttacatccta gaagttaata caggtgcatt tccacaagaa aaacacagct
     1861 tttcaataaa tgaatctgag ttagaaaaat tatactaata ttaaggatgg tacaaaatgc
     1921 ttgtaaaaga aattaaatta ctaagaaata taatcaaaga ttggagaaaa catggctatt
     1981 ctatagggct tgttacaaca atgggatttt tacatgaagg acaccaaagt cttataaaaa
     2041 aagctgtaaa agaaaatgat aaagtagtag tgagtgtatt tgtaaatcca actcaatttg
     2101 gaccaaatga agattttaat agctatccaa gagatataga taaagatttt aaatattgta
     2161 tggatagtgg agcaacagta gtctttaacc catcaccaga agaaatgtat ttaaaaggta
     2221 attgtactac cattaatgtt tctggactta cagattttct ttgtggagct aaaagacctg
     2281 ttcattttgg aggtgtctgc ctagttgttt caaagtttct aaatatagta acacctgata
     2341 aagcttattt tggagagaag gatgcccaac aattagctgt aattaaaaga atggtcaagg
     2401 atttaaacat agatacagaa ataataggct gtcctataat tcgtgaaaat gatggattag
     2461 caaaaagttc gagaaataca tatctctctg aggaagaacg aaaatctgct ctaatattaa
     2521 ataagagctt aagtttagca aaagaagaat tagtaaaagg caatctaaat ccggataata
     2581 taaaagaatt aataacagct aaaataaatt ctgaacactt agcaaaaata gactatgttg
     2641 aaattgtaga tagtgagaca ttacaacctg ttaagcaaat agaacattct atacttgtgg
     2701 caattgctgt gtttatagga aaaaccagac taattgataa ttttacattt gagttaaata
     2761 tatagtctat aat
//
LOCUS       DAFKMX010000297         2755 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFKMX010000297 DAFKMX010000000
VERSION     DAFKMX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.305"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            222..1640
                     /locus_tag="LZB08_003638"
     CDS             222..1640
                     /locus_tag="LZB08_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBM7317096.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1716..2528
                     /locus_tag="LZB08_003639"
     CDS             1716..2528
                     /locus_tag="LZB08_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7317097.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1027 a          278 c          485 g          965 t
ORIGIN      
        1 aatattaata aaaaaaaatt caaattataa atattaaatg aatataatta aaacaaatat
       61 ataaatttaa gtataacact cacattttaa tgtattgtga aatttttgtt gaactttatg
      121 taccataact tataaaattt atgtcatgtt atatttgaat aagtaataaa attgtagatg
      181 taaattaatc tacaaaattt atatgaaatg gagttgatgc tatgacagaa ttaatgatta
      241 taagtgggtt agtactattg atttgtataa cttctagcaa ggtattgtat aaatttggag
      301 taccaatatt attgattttt atcttactag ggatgttatt tgggtctgat gggatggtag
      361 gaatctattt tgataactat gaactgacaa aacaattatg ttctattgga ctaatattta
      421 taatgttttt tggagggttt ggaacaaact ggaatatggc taaacctgtt gctatacctt
      481 ctatattaat gtcttcactg ggtgtaataa ttacggctgg cgttacaggt atgttttgcc
      541 acatggtaat aggaactagt ttacttgaag gtttattaat tggggcaatt gcagcttcaa
      601 cagatgctgc atctgtgttt gctgtgttgc gttctcaaaa actaaactta aagggttcac
      661 ttgcatcatt attagaagtt gaaagtggaa gtaatgaccc aatagcgtat atgttgacac
      721 taattctttt gacattgatg aataattctg gggtagaact tattgcacca atggtaataa
      781 aacaaatagc ttttgggtta gctataggat ttatactagc aaaattatct gtttatattt
      841 taagacattc aaacttcgag atagaaggat tttatactat atttataaca gctattgcaa
      901 tactttctta tgcatttagc gagtatcttg gaggaaatgg atatttaagt gtttacattt
      961 caggtattat tataggaaat agtaggatac ctcttaaaaa aagtttggtt catttctttg
     1021 atggaatttc atggattatg caaataatgt tattctttat gttaggattg ttatctttcc
     1081 cttctaagtt accaaatgta tttggtatag caatggccat atctatattc atgatagtta
     1141 ttgcaagacc attagcaaca tttatagttc tagcaaaatt taaattctca aataaagaaa
     1201 aattattcat atcgtgggta ggtcttagag gtgcggcttc agtagttttt gcaatttatg
     1261 ctgttactca aggagtagcc atagagaatg acatattcca tataatattt tttatagcgt
     1321 tgctttcagt aggaatacaa ggttcattga taccaactgt agccagaaag ttggatttag
     1381 ttgatgataa tacacctgta ttaaaaacat ttaatgatta tgatggagaa attagctcta
     1441 aacttatcga agtaaatgtt gatgagggta ataaatgggc taataagagt ataatagatt
     1501 ctaatatacc agacgatata ttaatagtta tgataaaaag aaaggaacag gttttaatac
     1561 ctaaaggttc aaccattata aaaacagggg atattttggt attgagtgga gatagaattg
     1621 aagaattaat acaattataa tttattttgt attgacttta acctaagggg aaagtttaaa
     1681 cttaaattaa tcaaatctaa tgcgcaggtg atgagatgtt ttctatagga atgttttcta
     1741 aaataaataa aataactaca aagacattaa ggtattatga agatataggg ttgttaaaac
     1801 cagaatatgt agatgaattt acaaaatata ggtattatac aacagagcaa ttacctaaat
     1861 tacatcagat aataacttta aaacaaatgg gtttatcatt agctgagata aaaaaagctg
     1921 tagaaaatcc tgaagaagtt gaaaatattt taatgaataa agaaaaagaa atgttggatg
     1981 taattaaaag tgaagaatat aaaataatta aactacgtaa ttaccttatg aatttgaaag
     2041 gagaagtcaa tatgaatgat gtaattatta agtctttacc aaaagttaaa gttgcttcta
     2101 tgagaaaagt gttaaagggg tatgatgaac tttttaatct atttccaaat gtaatggctg
     2161 aagaaatgat gaaaacaggg tgtacatgtg cagttcctga ttattgtttt aatatatatc
     2221 atgatggtga atatagagag actgatatgg atgttgaaat gtgtcaagct ataaatgatt
     2281 taaaggaaga tacggatata ttaaaattta aagaattaga tgcagttgaa aatgcagttt
     2341 gtatattaca caatggtcca tattcaacat taggaaaaac ttacgcagat gcatttaaat
     2401 ggatagagga caataaatat aaagtggttg gaaatcctag agaatcttat atagatggta
     2461 tatggaataa agagagtgaa gaagactggt tgacagaagt tcagatacca gtgaaacgca
     2521 tgttataata tgtctacaaa gtataaataa gaataaaaaa ggtggtgtct caaagtaaac
     2581 attttagttt atgagacact ctttttcata tgaaatcaag tatattttta taattacgat
     2641 tatgaaaaaa ggcttatcta tatttttaga cagcctctta tttttttatt ataatagttt
     2701 tctaaagatg ctctatttat aaatttttat taaagacttt ataaataatt gtatt
//
LOCUS       DAFKMX010000298         2722 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFKMX010000298 DAFKMX010000000
VERSION     DAFKMX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.306"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            18..869
                     /locus_tag="LZB08_003640"
     CDS             18..869
                     /locus_tag="LZB08_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBM7317098.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="LZB08_003641"
     CDS             complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="LZB08_003641"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBM7317099.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLGLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
BASE COUNT          856 a          467 c          389 g         1010 t
ORIGIN      
        1 cacaggaggt acattctttg aaaaacatta tttcagcaaa agaacttata tcaaaactag
       61 aaaaaaatga taatctagtt gtaatagatt gtagatttga tcttatcaat agaacttatg
      121 gtattgattc ttataaaaaa ggacacatca aaggcagttt tatcttagat atagacaagg
      181 atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac cctttaatac
      241 taaaagaaaa attagaaaaa atgggtgttg ataatgacac tacgattgta acttatgatg
      301 atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg gggcttaaaa
      361 atgtctatgt actggatggg ggaataacat ctttcgtaaa ggaaggtgga gaattggaag
      421 agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata aacaatgatt
      481 tagttgttcc aatggaatat gttaagtcta aattatacaa aaaagatact gtaataatag
      541 attgccgtgc taatgagaga taccaaggtt tagttgaacc tgcttactca aaagctggtc
      601 atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac tttgaaaatg
      661 gttcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat aattatgatg
      721 aagtaatttt atcttgtggt tcaggaatat ctgcttgtgt taacagtctt gctcttagag
      781 aactggatat acctcacaag atttatattg gtagctttag tgactggatt agttatgatg
      841 acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat cagcatatga
      901 tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt cgcctttact tattatttca
      961 tatactcttt aaatttatta tatatatttt agttgataat attaataaac tgcaatatga
     1021 ccatcacatc ttgactccgt cccacaaata taactttcct gttctgtttt aaatattatt
     1081 tgacctcgtc ccatgtcgac agtatcatga attactttta tttcatgacc cttttcaatt
     1141 aatgaatcta ttatatgctt tggcatctct ggttcaactt ctatttcctt tccttttatc
     1201 cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc aatcatattt
     1261 gttaacactt gaatatgtcc ttgtggttgc ataaaagctc ccattacacc aaatggtcct
     1321 attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg ttttttaaat
     1381 ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct actatgtaaa
     1441 gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt gctctgtata
     1501 tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc accacaatat
     1561 ggctctcctg cagttggata caaagcctct ttattgtcaa tcaataaact tctttttttg
     1621 gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat atcagttaca
     1681 tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg tatgtcttct
     1741 acattttcta tatttccttc taattccagt tcttttaata tgttaagtgc cattaaaact
     1801 gttattccat gaccatttgg tgggatttca aatattttat atcccttata ttctgtactt
     1861 ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat agccccatta
     1921 aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa gctttcagcc
     1981 tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa tatatctcca
     2041 gcctctggag ctttaccatc tttagaaaat gtatcaaacc atggtttgaa ctcttctcca
     2101 ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac attaggagat
     2161 actacgtatc cctctcttgc atagtttatc gctggagata agcattccat taagccaagt
     2221 ttaccatatt ttttatttaa ttctgaccaa gctgctggta ttccaggtac agttactgct
     2281 ccaaacccat attttggcat ttctttcata ttattataat tttttaattc catattttct
     2341 ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat ccaaacaagg
     2401 gcatatgcat ctcctccaat cccattgctt gtaggttcta ctactgctaa tgtggcggct
     2461 gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag tccagcttga
     2521 gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac atttcttatt
     2581 gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc gtcatttttt
     2641 atttgatatt ctgcttagat ttattatata acatcaagta atgtaactga tacaaaatga
     2701 tataaaatat agactttttc ct
//
LOCUS       DAFKMX010000299         2715 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFKMX010000299 DAFKMX010000000
VERSION     DAFKMX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.307"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(399..1295)
                     /locus_tag="LZB08_003642"
     CDS             complement(399..1295)
                     /locus_tag="LZB08_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBM7317100.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1437..>2715
                     /locus_tag="LZB08_003643"
     CDS             1437..>2715
                     /locus_tag="LZB08_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBM7317101.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINFPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVICISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKA"
BASE COUNT          923 a          382 c          308 g         1102 t
ORIGIN      
        1 ataaaatttt ttataaatat ataatttttt tatatttatt tgactttctt ttaattcttt
       61 aggatggatt taaattttct ataaaaatca tactatttta gctgtttact tgcttattta
      121 atagttttat ttctttgatt aaatatcttt cagaatctaa gccagatgtc tcaaaaaaac
      181 tgatttataa tataatcatt ttttctgcta ttcttatttt tccagtttta gtttcatatt
      241 aaaatatact tttcatagac actgcattct ttcatactca ttttcacatt taactataca
      301 agtagtctta ctcatttatg tactatactt tattaatatt tattaagaaa aactcaaaga
      361 tttaaaaata tatattttct ataaagtata tttatgaatt atttcatatc ttcttttaaa
      421 ataacattac taacttctct atgttcattt ctatacttag ttggagatat attataattc
      481 tttttaaata agcgactaaa ataatttatg ctatcaaagc cacatctcat agcaatttct
      541 gtcatattta aatctgtttc ctttaaataa tatatagctt tatcaagcct gattccattt
      601 ataaaatcga ttgtagtctt acctgttatt tgcttaaatg ttctacaaaa gtgatgtgca
      661 ctcaagttca cacgactagc aagttctttt aatgtaattt tatccatata tttttcttct
      721 atcaaatcaa agatacattc aaatttttct aaagaatact ttctttttat aaattcatct
      781 tcagaatata ttttatcaat atgacttctt aataagagta ctattaattg atagatatta
      841 gcttttatag ataactcata tccaacttct ttattgtgat attcatcaag tatagttgta
      901 atacatttta aaatcagaga atcattttga attaaattat taaatacaat atgatttaaa
      961 gccaatggtg ctaagtactt ggtttgtaac aaatcaactt gattactaaa aaggaatgtg
     1021 ggttcaatac gaatactata gaattttaaa ttatctgata agctttctaa ataatgtaat
     1081 tcattactgt ttgctattac aatatttcca ggcactacct taaaattatc caatccacat
     1141 tcaagatatg ctataccatc aataaaataa tatatttgta gatgctcatg ccaatgtctt
     1201 ttaaagtttg ctcctttttt aatgtcatcc tttaaaaaaa tatctataaa gttttcttga
     1261 cctttcctgt taaaatatgt atttttatcc ttcattattc ccccctcatc aaaatagtgc
     1321 taattataag caatatagtt attgtattca ttatttcata tcgctattat ataaaatgta
     1381 actatcattg taaacattaa cataatgcca gtcattataa taaggaggta ataaaaatga
     1441 caaactcttt taaaaaagat gataatagaa aaaaaatcac agttttattt gtagtcattg
     1501 ccatgacttt tatggcaaca ttagattcta gtattataaa tgttgctctc ccagttcttg
     1561 caagcaaact aaatgtatct ctagcatcta ttgaatgggt aatagcaagt tactcaatta
     1621 ttatttgttc aacactatta ttttttggaa gacttggaga tattattggt aaatcaagag
     1681 tatttcaagt aggtactatt ctctttacat cagcttcatt actatgtgga ttaagtaatt
     1741 ctttaacttt acttatagtt tgccgcttta ttcaaggaat aggcgcttct gcctacatgg
     1801 caaacaatca tggaattatc acagaactat ttccaaaaga aagcagagga aaagccctag
     1861 ggattttagt tactgcagtt gctattggaa atatggttgg accatctgtg ggaggattta
     1921 ttttgtcaat atttgattgg aatgttatat tttttataaa ttttccaatc ggtcttattg
     1981 taatcttttt aaatacaaaa tttcttccta atagtaaaaa atcttcagaa aatatggata
     2041 aaactggtgc tatacttcaa tttttaggaa caacattatt ttttagtgct ctaattagtg
     2101 ctcaacaaac aggattacta aatccatata tattaattgc actattatta tcaattattt
     2161 ttattatatt atttttgata ttagaaaaaa aacatcctca accgcttctt gaccttgaaa
     2221 tttttagaaa ttttaaattt tcattgaatt taatttgtgc tcttacatca ttcatttgta
     2281 ttgcatcatc aagtatttta attccatttt atttacaaag tactatgaaa cttcccccta
     2341 ttcaagcagg tttatttatg atactatcac ctttgatttt agccattttt tcacctatat
     2401 ttgggaatat atcagataaa attaagtcag aaaaaattat tttaattggt cttctcgtta
     2461 tgagttttgg attctttcta atgtcaagat tgaaagaaag ttctgccctt attttatttg
     2521 taatttgcat atcgataata tcaataggac aagctatatt tcaacctgct aacaatgctt
     2581 tgataatgtc aagctgttct agaagtaagc taggagttgt aggaagcata aattcccttg
     2641 taagaaattt aggacaagtt ataggtatta ctatttctac aacactttta tataatttta
     2701 tgagtattaa agctg
//
LOCUS       DAFKMX010000300         2655 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFKMX010000300 DAFKMX010000000
VERSION     DAFKMX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.308"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            406..2217
                     /locus_tag="LZB08_003644"
     CDS             406..2217
                     /locus_tag="LZB08_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBM7317102.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAVLSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQIENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2305..>2655)
                     /locus_tag="LZB08_003645"
     CDS             complement(2305..>2655)
                     /locus_tag="LZB08_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317103.1"
                     /translation="GTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNFPNNEVKDY
                     IQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSK
                     NIEEFLYKYKKRFK"
BASE COUNT         1001 a          273 c          393 g          988 t
ORIGIN      
        1 ttacataata tgataaaata aattcaaaga gttaaattgt aggtaaatag tattttaatt
       61 atttgttaaa atatagatta tgtatatgcc aaatttaaaa agagacataa agacattata
      121 caaatatatt attggggtga tattttataa gaaaagaagt gatgcagtaa attaattgag
      181 caatttaaaa atgatttaat gatagtgttg atgaatgttt aaaaagaaat tttagaaaaa
      241 cgtatattgc tgtttgtgtt ggaggtgatg aatttgtata atttatagga tataaatgat
      301 taaactattt aagataaata catatcactg taaaatattg ataatcattt tttataagag
      361 attgattatt tgcaaattat atatattaca aatgaggtaa caataatgaa gaaaaaatat
      421 gattttttaa tttattctgc tatttggcta gttattatta ttctttttat attttctcta
      481 tatcatagta tagagcatat taaaattata agtcacactg gtactattcg cagtgaaact
      541 caaaaggttg ttaagcaaga attgaataat gaaagaaatg atgacttgat aaaacgactg
      601 gataatattc tgataaagct aagaacaggt aatggagaaa atggatttca aagatgtgat
      661 aataaagaat ttcaacaaaa actaaatcaa atggattcta tgtgggaatc tatgaagaaa
      721 gaaattataa aagttagaaa tggtgcttca ggtgataaat tatataaatt gagtgaagag
      781 tattctgtgt tgtctaatca aattgttttt atatctgaaa aacactctaa tgcaaaattg
      841 tattcttttg ctactgcact atttatttat cttatattct caacaataag tcttttaatt
      901 tgggaatatt ataataaaaa aaggtttaaa agaatatttt atactgataa tttaacaaaa
      961 ataaaaaatc aggttgcatt tgaaaacaga gcgattgaaa ttttgcacaa tgcatctaac
     1021 aaagagtatg tactgctaaa tattgatatt gacaatttta aatatattaa tgatacacat
     1081 ggatatgaat atggagataa agttctaata atagttgcag ctgtactttc taaaactttt
     1141 aatatcaaag aaacttgtgc aagaattggt tcagataatt ttgtaatact tgcaaaatat
     1201 agagattctc ttttagagga tattcgggaa atgctaacta atgctataat ctctgaatta
     1261 gatatgaatg taactcaaac tatatcttat tgtataggag cgtacttagt tgaaattgat
     1321 aatttaggtt ataaatccat caattctata atggataagg caaatattgc acacaaggta
     1381 agtaaaacaa gaggaatttc ctccacagta tggtataatg aaaatctgtt aaaacagtta
     1441 caaatagaaa atagtattta taattatatg tataaagcga tagaaaatga agaatttcat
     1501 atgtatttgc aacctaaatt tcaaatatct tctttaaatg ttgtgagcgc agaagctctt
     1561 gtaagatggt tttctccaga acttggtttt ttatcaccag atgaatttat tccattgttt
     1621 gaaaaaagtg gatttattat tgaattggac ttttatatgt taaaaaaagc gtgttcattt
     1681 gtaagaaaaa cgtttatgaa aaaaaatcaa tatacatatc cgattgcagt taatttttca
     1741 agggttacta tttaccaaaa tagtttttat caaaggtttt tagatactgt taaagaatat
     1801 gaaattcctt ttaagtatat agagatagag gtaactgaaa gtgcttttaa tgaaatatct
     1861 caaccagtta tttcaatttt agaggaactt aaaaagttag gatttttaat ttcaatggat
     1921 gattttggtt caggatactc ttcattaagt cttttatgtt ctctatctat taatggattg
     1981 aagttagata aaagcttact taaagaaacc tttaaccggg agaaagtata tagtattatt
     2041 cagtgtatta ttgaaatgtc acatcgtata ggcatgtctg ttgtatgtga aggtattgaa
     2101 actaaaaaag acttggaatt tttaaataca gttaaatgtg atgtaggaca agggttttat
     2161 ttttctaaac cgatagaaga aaaagaattt tttaacaaat atgtaactaa aaaataatat
     2221 aattattttt atcagtctat acattgagtc attttatatc tcgttaaatg actcaatgta
     2281 ctgttatgta ttttaaaata taaattattt aaaacgtttt ttgtacttat atagaaattc
     2341 ttctatattt tttgattgtt ctcttatttc aactgcttct atatctagta aataaagtat
     2401 agaatcattt gtaagttcaa catattcacc atcttctgaa ccgagtgcat gtctctcaat
     2461 ttttaatcct ttttcttcat aatatttttt tgtttctata tatagtgatt ctcgcttttg
     2521 tatgtagtct ttcacttcat tatttggaaa gttatatgaa tccactaatg tttcgccatt
     2581 tataaaagtt ttctcggtag caactttttc atcccaacaa tggatttctt cccaagtaaa
     2641 aaatatctca gttcc
//
LOCUS       DAFKMX010000301         2642 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFKMX010000301 DAFKMX010000000
VERSION     DAFKMX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.309"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..848
                     /locus_tag="LZB08_003646"
     CDS             <1..848
                     /locus_tag="LZB08_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBM7317104.1"
                     /translation="FPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNIIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(1065..1631)
                     /locus_tag="LZB08_003647"
     CDS             complement(1065..1631)
                     /locus_tag="LZB08_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBM7317105.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            1797..2318
                     /locus_tag="LZB08_003648"
     CDS             1797..2318
                     /locus_tag="LZB08_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBM7317106.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
BASE COUNT         1138 a          270 c          390 g          844 t
ORIGIN      
        1 gatttcctac tgacagtgaa ttttcaaatg cttttgaaac taaagatata tataatacta
       61 agtcaaagaa taagttatat atacttgaga ggttagagaa ttataataat aaagaaattg
      121 ttgatttaga aaatttaatc aataataata tgctaaatat agagcatata atgccacaaa
      181 cattaacaaa taaatggaag gaatcactag gagataacta taaggagata catgataaat
      241 atttacatac aattggaaat ttaactttga caggatataa ttcaaaacta agtaataaaa
      301 catttgaaga aaagaaagaa atggaagatg gatttaaaga tagtaggctt tatctaaata
      361 aatatatttc atctatagat aaatggaatg aagaagaaat aaaaaataga actaagatat
      421 tactagatag agctgtagaa atttggaaat atccaagtgt tacatatgaa cctccaagta
      481 acagtgaaaa tatatttacg ttagaggatg atgatgttaa ttttacaaat gaaagtatag
      541 tatcatttag tatattagga gagtcatata aggctaaaaa ttggacagaa ttttatgaac
      601 aagttgctct tactttatat gatttggatt ctgtaaggtt taacattatt gtagacaaaa
      661 cttataaaag agattttata gataatatta agtctagaga tgcatataaa ttaagaaaag
      721 gtattaaaat atcagaaaat atatatttag aaacaaattt aaatacagag gctaaattaa
      781 atataattag atttttactt aatgagtttg atatagagtt aaatgaagta atattttcca
      841 taaaataatg aaatatatgc attttttgcg taatagaata ttaattatat taaaaataaa
      901 atagcacctc ttgatataga tttatagctc aaatttatat caagaggtta cgtttaatta
      961 aagtaataca aatactatct aattaaatta ttaataaaaa tgttatataa aaatgagtat
     1021 atagcatttt aaagagtaat aaaccttaca taataaatga tttattaata tgaactagaa
     1081 gtatttttac tatattgttt tataaaacct ttaggcattt tacttataaa atataccacc
     1141 ataatacaag acaaaggttt gtctactata ttactaagaa tccttggaaa gaacgcagca
     1201 gtaaatatct tctgaccaga ctgaactaaa aaaccaacca ataaatcagc accacttcca
     1261 cttaaaccac caaacaataa aactgaaata ggagtaccaa ttaaaggaca aacaatagat
     1321 aaaattactc ctgtaataat agcttcttta taaccaaaac cttttttctt agaaatatat
     1381 ccaacaataa ggccaagaac aatatttaca agggcaaaag gtatatttgt aggacctgat
     1441 ataacaccta aaactaagtt tgtacatcca ccaataagag caccccaaaa aggacctaat
     1501 acgacagccc caagtacagt accaactgta tctaaaaata ataatggtat atttatcata
     1561 gatataacca tacctaagac aatattaata actatgcaca ttgaagaaaa tgtgactttt
     1621 ttagtatcca attataatac ctcctaaaaa aatatataca tttatcatag tatagattat
     1681 aaaagattac taattgtata tttctatata gaatattgga aatatagaac aaataaataa
     1741 tataattatc aaaatatgta gtatcattaa aattaagaat aactaaggag attattatga
     1801 aaagaaatag agagattgta gtagtatgtc attgtatatt aaattgtaat tctaaagtgg
     1861 agggattgtc agaatttaag ggagcacaaa atatagcgat aaatttaata aataaagggt
     1921 atggaataat tcaactacca tgcccagaaa tgattatgta tggaataaat aggtggggac
     1981 atgtaaaaga acagtttgac aatttgtttt ttagagaaca atgtaggcaa atgttaatac
     2041 ctcatgtaag gcagtttgat gaatatttaa aaaatgatta tgatataaaa agtatcattg
     2101 caatagatgg cagtccaagt tgtggttata ataagacatg tagttctaaa cgttggtttg
     2161 gtgagttaag tggttgcaat gaattgatgg ataaaataaa tgatatacaa atggtggatg
     2221 ggaaaggtat ttttatagaa gagctagaaa agctacttaa agaatataat ttaaatatac
     2281 ctatagttgg atttgacgag cttagagaag ataagtagag atataaatat ttattgtctc
     2341 attatgaaac accattatat ttggcagaaa aatatgactg atggacagta cctgaatcat
     2401 ttttaagtgg agtaattagt acttataaag atgtatccac tagttgaaag aaaatgtaga
     2461 gaggtaataa atgaattaag aagtagataa tatatataaa ggagtgtcta aaagtgaact
     2521 ttttagttta tgagacactc tttttttata ttaaatcaag tatattaaat caaatatatt
     2581 ttaatcattt tagcaatgaa aaataaattt atctatattt tgagacaaca tcttttttag
     2641 tt
//
LOCUS       DAFKMX010000302         2631 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFKMX010000302 DAFKMX010000000
VERSION     DAFKMX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.310"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            151..654
                     /locus_tag="LZB08_003649"
     CDS             151..654
                     /locus_tag="LZB08_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBM7317107.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            701..1660
                     /gene="moaA"
                     /locus_tag="LZB08_003650"
     CDS             701..1660
                     /gene="moaA"
                     /locus_tag="LZB08_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBM7317108.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1664..2131
                     /gene="moaC"
                     /locus_tag="LZB08_003651"
     CDS             1664..2131
                     /gene="moaC"
                     /locus_tag="LZB08_003651"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBM7317109.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKF"
     gene            2170..2601
                     /locus_tag="LZB08_003652"
     CDS             2170..2601
                     /locus_tag="LZB08_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBM7317110.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1100 a          243 c          516 g          772 t
ORIGIN      
        1 aataaaaaat gtattattat aggtgatata aataaaaaat aaatataata tgttatattg
       61 ctgatagaaa agagaataaa agaaaatagg ttttattaaa agagattttt atttatagga
      121 cttaatcaat gtatagaggg gtgagaacat atgtttaatg ttgcaattat aacacttagt
      181 gataaaggat atgaaggaaa aagagaagat ataacaggaa aaaagttaac tgaatttgtt
      241 gaaaatacag gagcttataa agttactgaa tatgtactta taaaagatga taaggaaatg
      301 ctaaaggaaa atattataag actttgtaat agtaataaaa tagatttaat tcttacaaat
      361 ggaggtactg gtttttcaaa aagagatata accccagaag caactaagga agtgttggaa
      421 aaagaaatac caggtctgag cgaatatatg agaatgaaat ccacagaaat tactaaaaag
      481 gccatactaa gtaggggtgt aagtggaata agaaataatt ctataataat aaatttacct
      541 ggaagtccta aaggtgcagt tgaaaatcta agttttataa ttgatgtttt agaccatggc
      601 atagaagttt taagaggaga agctacagaa tgtgccacta aaaaaagtga atagtatata
      661 atctagatag ataaaaaata gacaaagagg tagataaagt atgattgata agtatggaag
      721 aaaaatagac taccttcgta tttcacttac agataagtgt aatttaaggt gtgtttattg
      781 tatgccacca gatgtaaaat ttgataaaaa ttatataaat gaaaatctaa gctttgaaga
      841 ctacaagttt attataaagg ctatggctga acaaggtata acaaaaatta gatttactgg
      901 aggagaacct cttttatatc ctaaattaaa tgagcttata aaatttacaa aagaagaatg
      961 tggaataaat aacataggaa tgaccactaa tgctattggg ctttctgata gaatctttga
     1021 gcttgaaaaa tgtggcttaa ataaagttaa tataagttta gattctttga aggaatataa
     1081 gtataaatcc gtaacaagag gaggaaactt aaaagatgtc ttaaaatcga ttgaatcttg
     1141 tttaaactta ggtataaaag taaagttaaa ttgcgttgct ataagtgggt ttaatgatga
     1201 tgagcttaaa gattttatgt ttttaacagt taaatcacca attgatgtaa gatttataga
     1261 gcttatgcca ttaggtgagg ctaatagagt ttataagaag ggttatttta atgtaaaaga
     1321 aatgattgaa aaaatatatg gtttatataa agtcaaaaat gatgaaaaaa gtacagcaga
     1381 atattatatg tttaaaggag ctaaaggaag aataggaatt ataactccat taagctgttc
     1441 attttgtaac acttgtaatc gtattagact tacttctagt ggtgcaataa aattatgtct
     1501 acattcaaaa gaagaaatag atataaaacc atttttacat aaaccattgt tatttagaga
     1561 atctatgaaa gatataataa aacaaaaacc agaaaaacat catttaatag aaaataaatg
     1621 tagtgataca aataggcgta tgtatgaaat tggaggataa gtaatgttaa cacatataaa
     1681 tgaaaaaggg tatgccaaga tggtggacat aagtgaaaaa gatgatacta aaagaacggc
     1741 cgtagctaca gctacaatta ctcttagtaa ggaagcatta gaaaaagtaa aaaatggtca
     1801 gataaaaaaa ggagacgtat tgtctgtagc tcaagttgca ggtataatgg gagctaaaaa
     1861 tacatcttca aacataccaa tgtgccatca gattaaatta gttggatgtg atttagaatt
     1921 tgatgtagat gattctatgt ctgctattaa gatatacgca acctgtaaat ctcttgggaa
     1981 aactggagta gaaatggaag ctttaaatgc atgttcttta gcagcactta caatttatga
     2041 tatgtgtaaa gcagttgata aaaaaatggt tataagtaat atccatcttg tggagaagac
     2101 tggtggaaaa tcaggagatt ttaagttcta gtaaattaat aatatattgg gttaaaattt
     2161 ggaggaaata tggcgaaggt agtgtcaata aatataagtg agaaaaaggg tgtaattaaa
     2221 gttcctgtac aatgtgcaga gctaaaaata aatcatggga tagttggaga tgcacatgca
     2281 ggaaactggc atcgtcagat aagcttactt ggaaaagaaa gcatagataa aatgaaaatg
     2341 aaaggctttg accaattaaa atatggagat tttgctgaaa atgtaactac agaagggata
     2401 gaagtttttt ccctgcctgt tggtacaaag ttaagaatag ggcaatgtga agttgaagta
     2461 actcaaatag ggaaaaaatg tcacaatgga tgtgaaatta agaaattgac aggtgactgt
     2521 gtgatgccac gagaaggaat ttttgtaaag gttctaaaag aaggaattat aaatgtgaat
     2581 gattcaatag atgtaatcta atgaagtcac tattaaaata taattataag t
//
LOCUS       DAFKMX010000303         2608 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFKMX010000303 DAFKMX010000000
VERSION     DAFKMX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.311"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            446..925
                     /locus_tag="LZB08_003653"
     CDS             446..925
                     /locus_tag="LZB08_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7317111.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            922..1701
                     /locus_tag="LZB08_003654"
     CDS             922..1701
                     /locus_tag="LZB08_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317112.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1951..2451)
                     /locus_tag="LZB08_003655"
     CDS             complement(1951..2451)
                     /locus_tag="LZB08_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7317113.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         1020 a          358 c          316 g          914 t
ORIGIN      
        1 tgacatttct aactcttaat tctcatttaa gtttcattgt tttttcttaa aagcctactt
       61 ttagtcaaga tactgttatc tttactcatt atttaaagaa cttattttaa atctgtacat
      121 gaagtaaata atccaaaagc attgttattc taactactca acattaaata cttactattt
      181 tctaattact caatataaag acttactata aaactaaata cataaatggc ttctaaagtt
      241 taatatatct acatggcttt atatatgaca ggaaaaatac tagaaaaaat atattataga
      301 atacttgtat ataataggca aaactttcaa acttaaattt atcaaataat cataaaaaaa
      361 gtgaaccatc tatagtaaaa ttttatctaa taagtgaaag gcaaaaaagt agcgctacga
      421 tttgcataga agggaaggaa cttagttgga taaaactaca tttacaaaca atatacttga
      481 atcagaacag actttgtatc gagtatctaa gtctattcta ggaaacgacc aagactgtga
      541 agatgccgtc aacaatgcta ttttaaaagc atatgaaaag ttggattctc ttaaggaaga
      601 acaatacttt aaaacatggc tgattagaat agtcataaat gaatgtaatt cattaagacg
      661 caaacgatta aaatctctat catttgaaga tgtcttcaaa aacaaaaaaa ttgatgaaaa
      721 agatgactat agcgatttgt atactgctat tcaaagtcta cctaaaaaaa ttaaaattcc
      781 aatagtcctt tactatattg aaggttattc agtagatgaa gttaaggaaa tattagatat
      841 acctcaagga actgtaaaaa gtagattatc aagaggaaga agattattaa aaactaaatt
      901 agaaaacacg gaggtaatca tatgaataaa aatatattaa ataattcgtt tccagaagtt
      961 ccagaaagtt ttcataatag tttaagtaaa acactaaata gtttaccaga aagagaggaa
     1021 aattataaaa tgattaataa taaaacacac aaattaccat ttagaaaagg cttagttgca
     1081 actttagcca ttactttgat attgagcaca acagcccttg cagtaggaca tatatatagt
     1141 ttggttggaa aatcaagtac taaggctacc tatacatcta tcccaactac agaacaagta
     1201 aaaaaagact ttaactttac ccctaatata gtttctgaat ttagtaatgg atataaattt
     1261 aaaggagcat atactgtaaa taaaaaagca tttgacaaag aaaataatgt tttaggaaaa
     1321 tcaaaagcac ttagttttga atataaaaat ggcaatgatg aaatatctct tgacacagca
     1381 aataatgtac ttgggaagag ttctgacaat ctaaaagttg tagatactta caaagatatt
     1441 aatatttctt atgaagcatt cgcacaaaag tttgtaccag aaggttataa aatgacagaa
     1501 caggataaaa ttgatgaaaa atcaggcaaa tacacattta gtgtagggcc aaataatgaa
     1561 tcagacaaag ttgaagttaa taaatttaag tttttgacat ggaaacaaga tggagtttac
     1621 tattctttta cagttcaaga ctcaaattta tctatgaatg acttagttaa aatggctcat
     1681 gaagtaatag atgcaaaata aacataagta ataataagtt gtttatgagt ttattattta
     1741 tgactttaat ttttttatta gtaaacatgg ttgtatcttc catctttata ttttctttaa
     1801 atttaatata taaaatttta gatatttagc taccttcaaa agaaccatta ttaaaaagcc
     1861 cttaaaaata tttcttttat atggaatttt aagggcttta actttatttt atacaagtgt
     1921 atatggaatt ttaaagactt taactttatt ttatataagt gtaagctcca tttgaattac
     1981 ttcttctttt acaggtctat cattccatgt aaaaccacta gatattgtat ttacctctac
     2041 aaaccctaaa gatttataaa catgaatagc tctactatta cttttaaata catcaagctc
     2101 aattttttca tacccatatt cttttgataa atcaataatt tgttttaaaa gactacttcc
     2161 gatacctttt tccctatact ctttaagaat acccatgcct aaataaccaa ctttttcagt
     2221 tttaggtaaa acgtcacacc aaccaataca gttatctgat tctaaatcaa ttataaacag
     2281 ttgtggtaag tttttattta taatattttt tataaattca actgtttctt caaaaggaaa
     2341 ggcttcatcc atagctagat atttcctctc ttttgctatt ttatcaaaag tttgccaata
     2401 cgaataaata tacttttctt ctgcctttat aaattttatg ccttctatca acttaacatt
     2461 tccccttttt aatttttgat tgtagttaac ttattttata tgattctatt ttataatgct
     2521 atataaatta ctatatatta gcttaaaata tctttgtata tttctataaa attaaaacca
     2581 atatatctat ctcttgtatc tctattaa
//
LOCUS       DAFKMX010000304         2523 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFKMX010000304 DAFKMX010000000
VERSION     DAFKMX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.312"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(272..1564)
                     /gene="brnQ"
                     /locus_tag="LZB08_003656"
     CDS             complement(272..1564)
                     /gene="brnQ"
                     /locus_tag="LZB08_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBM7317114.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          856 a          392 c          256 g         1019 t
ORIGIN      
        1 attttttctt tacaaaaata tgtattgatg tttttatatt aatactaaac atttataata
       61 tataatttaa ttttatttat aattataaat gaatcttttt tattttatta tctttataaa
      121 tcatactact ccaaaaataa atttttaaat ttatcaaata ggattaataa tgttctttta
      181 tctacaataa tttaataatt cttttaaata taaaaaggaa ttgcctatat aatttaaagg
      241 caattccata attatcaatt agtttttatt attatgcagt tttactgctt ttagatggaa
      301 taaagtttcc tattattcct gctattacaa ctggaactat ccagttaaat cctaatgaag
      361 caaatggtaa gctattaact agaggaattt ttataccaaa acttgttgca actgttaata
      421 cacttactaa taatgccatg tatgcagcac atttgaacac gttgtcattt ttaattttat
      481 tactaaataa tgctagtata actagagtta tagttgctgg atataccata cttagtatag
      541 gagaagagaa ttgtattatt gtacttactc caaagtttga tatgattgca ctaaatacac
      601 aaactactat aactatatgc tcatacttta attttccatc tgtaagtgtt gagaaatatt
      661 gaccagctgc tgaagaaaga cctatagctg ttgttaaaca tgctaatgca actataatag
      721 ccaataaaat tttacctgta gttccaagta aagctgctgt tattgcaact attaaagctg
      781 tttgttgaac atcttgtcca tattgttgag acacagttgc tccaagataa cataatccac
      841 cataaactaa tgccattcct attactgcaa ttacacctgc acttattgtg gctttcatct
      901 tttctttctc acctgtataa cctttagcta ttactgacat gattataatt gatgcaaata
      961 cactagctgc caatgcatcc attgtttgat aaccttgagt tataccttct gcaaatacat
     1021 tttgtattaa agaactgtct cttatttctc ctaatggaga tacgatacct ttaactatta
     1081 atactgctaa agctataagt aaagctggag ttaatattga cccaactata tctacaactt
     1141 ttgatggtct tattgttaaa accaatacta gagcaaagaa tactatagag aatattactg
     1201 ggcttattcc agaacctatt gtaggtaata tacccatttc ataagttgtt gctgctgttc
     1261 taggtattgc taaaagtggc cctatacaga ttaccattgc acatcctaat gcaatactta
     1321 atcctttgcc tgctcttgag aacatcttcg atgtgtctcc attgaactta gttgctgcta
     1381 taactgctaa taatgccaaa ccaccatctg caaaaaggaa tcctatgaaa gctactagcc
     1441 aactacttcc agataaaact cctagatatg gagggaagat taagttccct gcaccaaaaa
     1501 acattgcaaa tagtgcaaat ccaattacaa ttacatcttt aaaattgatt tgtttctcac
     1561 ccatttaaag cacccccatt tgaattttac taccttttac taaaaaataa gtccaatctc
     1621 ttaattaaat gaaaaccttt tgacgagatt ttatttaatt atacattttt gacttaaatt
     1681 tgtcaattat gtttcataat ttataaaatc ttcacaatat ttaatacaac tttcttaatt
     1741 aaacaaaatc aacctgtata ctttaaacat agttggtttt ttgttttttc tctaattttt
     1801 cttgataaaa ttaactattc cgaataatta aaatattaag actttttaga ttattcgatt
     1861 tattattaag ttgtattgct ttatatactg tatatatcac tctccttttt tacattcata
     1921 taatcttttt aattcaattt aaattttaaa tattttgtgt ttattatata atctgaaaaa
     1981 aaagcagtca atagcatttt taattttttt aattttcaaa attatgattc ttaaaaaatc
     2041 gttttccttt tatcataaca cctatttacg ttaattcttg tagtattttg tatttatttt
     2101 gctaaattga cagttaactt tattcaattt ttattctact tgcgtagatt tgcctcttgt
     2161 tttttactct taaaaccatt ttttctgaaa attccttatt atgatttatc aacttatttt
     2221 tcttcataac aattactctc taacttccaa cttacttaaa ctttgcagtt gctagtattc
     2281 acgtttacat acttatatgt cataattaac taaaaattct taatatatct ctcaatgaaa
     2341 acaatttact catttattta atacatttat ataatcatat gtcttaaatt ttgttttatt
     2401 attttattat tgtcacaatt ttatttatat tatatttttg taaaaatcat ttaatatgta
     2461 tacttaaatt tataaaatta ctctaaaatt cttttttaat ctctattatt gttaaaaaat
     2521 aaa
//
LOCUS       DAFKMX010000305         2469 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFKMX010000305 DAFKMX010000000
VERSION     DAFKMX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.313"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(149..1399)
                     /locus_tag="LZB08_003657"
     CDS             complement(149..1399)
                     /locus_tag="LZB08_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBM7317115.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1431..2372)
                     /locus_tag="LZB08_003658"
     CDS             complement(1431..2372)
                     /locus_tag="LZB08_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBM7317116.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          728 a          483 c          236 g         1022 t
ORIGIN      
        1 ctatctatat cctaaaatta tccatttcta aaaaaaaggt aactattatt aagaatatca
       61 acttatagaa tttatattcc acatacgatt ttcaatcaaa aaaacaaggg ctaaaaagtg
      121 aattgtgcac tttttagccc tttattaatt attcatttat ttcatgtaag aatagaccac
      181 atcttacttt aggttcaaac catgttgact tagctggcat tatcttattt gcatctgcta
      241 ctcttattaa atcttcaata ctagtaggat acattgcaaa tgctactttc atatcttcat
      301 taactctttt ttctaattct tcaagacctc taattccacc gataaaatca attcttttat
      361 ctactctagg attatcaatt cctaaaatag catcaataac atagtcttgt aatatagaaa
      421 tgtctaatga gtcaacaggg tcttctattt ctaataaatt tttatttgct ttcattttat
      481 accacttatc acctaggtac attccgaaag ttcctttttt gtcaggttta tatttttctt
      541 ttccttctat ttcttctagt tcaaaatttt cttttatttt atttataaat tcttcctcgc
      601 ttaaaccatt taagtctttg ataaccctat tataatccaa aaccattaaa ttctcatctg
      661 gtgcaatcat tgctatgaag taattaaatt cttcttttcc agtataattt ggatttttag
      721 ctctcatttc tttggctaca ttttctgctg aagctgtcct atgatgacca tctgcaatgt
      781 atagataatc taatttttca aatttatcta ctaggttatc tattatatca tcattatcaa
      841 tcttccatac tgtatgctct atcttgtcat ctgttatgaa atcatataaa ggttcattat
      901 tatccataaa atcattgatt atatcatcta tcaccctttg atgtttgtaa gttacaagta
      961 tagttcccgt attagcatca cagtatttta tgtgtttagt tctatcaatt tccttttctg
     1021 gtcgagtgta ctcatgtttt tttattgttc cattcaagct ttcatcaact gatatacatg
     1081 ctactatacc aacttgagct ctaccatcca tgatttgttt atatatatat atacactctt
     1141 tgtcatcttt tactagaatt tcttgttttc gaaactcatc taaattttct cgcgctttta
     1201 aataaacttt ttcatcatat aaatctatat tttcatctaa atcaatttct gatttatcta
     1261 tatgcaaaaa tgaatatgga ttcccttctg caattcttct agcttcttta ctattcataa
     1321 catcgtaagg aagtgctgct actttatcta ctatatattt atttggtcta atcgccttaa
     1381 atggtttaat cgttgccatt actaatttcc tcctaattat acaaaatatt ttataatgct
     1441 acccctatta gattgttgaa gtcaagaaag aaatcttgaa tcacatttac tatttcgtct
     1501 cctattcttt cttgagcttc tactgtagat gccccaatat gaggtgtagg agaaactctt
     1561 ggatgattta ctaagtctaa atttaatgta ggttcttgtt cataaacatc tatagcagct
     1621 gctgataatt taccttcatc taatgctttt agtaaatctt tttcatcaac taaaccgcct
     1681 cttgcacagt ttattaaata agcccctttc ttcatcttat ttatttcttt ttcagttatt
     1741 actgccccct tattcttatc aaatggtatg tgtaaagata agaaatctgc attttctaaa
     1801 acttcttcca tatcacagaa tttataattg ttaaagcctt gcgcctcacc cattatgtca
     1861 gtatatataa cattcatacc taataattct gctctctttg caacttcttt tgcaattctt
     1921 ccaaaaccta taagtccaag tgtctttcca ttaatttctg tacctttata ctttttcttc
     1981 tcccattttc catctctcat agttacattt gcagtattta tgaatcttgc aagaacaaat
     2041 aattgaccta ttgtaagctc tgcaacagat atactgcttg catttggagt attcataact
     2101 tttatgccat tagcttgagc atatttaaca tctatattat ccagacctac tccacctctt
     2161 actatcaact tcaacttttt accctctaat gctttatcta ttacatcttt agtaacttta
     2221 gtagctgaac gtactacaag tacatcaaca tctttaagtt tatctcctaa aacatctttt
     2281 tcataaaact gctctatcac attaaaatct aactctctca attttctcgc tgcttctttc
     2341 tctattccat cagtaactaa tatattatac ataacaaact cctccaattt ttattcactt
     2401 aatttttcca atttttattt tttaaagtat tttcatcaat aaaaaacttt acttaattca
     2461 actttctgt
//
LOCUS       DAFKMX010000306         2466 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFKMX010000306 DAFKMX010000000
VERSION     DAFKMX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.314"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            329..2224
                     /locus_tag="LZB08_003659"
     CDS             329..2224
                     /locus_tag="LZB08_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBM7317117.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     VDLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGRNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1079 a          246 c          448 g          693 t
ORIGIN      
        1 agatagatat taatttagtg atatatattt aagtaataag agtaaagcaa aaattagtgc
       61 atagtagtat ataaaaatgg agtttgaata cttttgttag tatatgattg tttagtagaa
      121 aatcactata aaataggatt tctatctttg agcataatta tagagagcta agtaggggga
      181 aggggttcaa gaagatatta ttagtgtttg tgttagtaaa ttatgtaaac acataaaata
      241 tattgaaaaa taatattata tataatatta tttatgtgtg ggaaatacga atataaaaaa
      301 taaaaaataa tattcggagg acataaatat gttaagcaac aaaaagagaa gtatggcaat
      361 agtaatggca ggtgctacag ttatgagtgc agcagcacca atattcgcag acaatactgt
      421 gacagaaaat gttgataaaa attacacagt aagtgcgaaa gattctgcta aactaatcga
      481 agaggttaga aaagcattag aagttaagtt tgaagataca aaagcaggag caaatgtaaa
      541 tgatagagtt tacgatataa aagtagacaa tgtaaactta actaatgcaa ctcaattaca
      601 aaataaaata aactctttaa cagaagggca aagtttaaaa gttactattc aagataaagg
      661 gcatcaagtg ttaggtggaa aagtagttga ctataaaatt gaaaactaca aaactgctca
      721 agaaatagta gatgcagtta atgcttacaa tgcaacttta gcagaagatt ctgataacaa
      781 gttaacagca actataaaat ctacaaatac agttgaagta aaaagagcaa aagattcagc
      841 aaatgttatt acattaaatg taggagacca acatttagat ttctcaaaag tgataacatc
      901 agaagaaggt acttttgaag gttatgaaaa gagttatagt gacatagatt caaaagaatt
      961 acatacgata acagttaaaa acgtagattt acaagacata tcagcagaag aattatttga
     1021 tggaattaga ttaactacat taggaagaga aatagttaat aaagttaaaa atggatatgc
     1081 tttaacattt gaaaatgaag caatccttac tcaagaacaa gaagatagtg atgataaaga
     1141 taaaccagaa aaatcaagct ttgatatagt tttaagcaag gcaaatgaaa agccagaaac
     1201 aataagtgtt tcaagtaaaa accataagtt agttagagac ttacacaaag tattaactga
     1261 tgtaaaagat ggaaaagaat taaaagtaga agttttatct ggtgattcaa gatttacaac
     1321 agcagtagaa gttagtaaag agagattcaa agatggagaa gcagaagcta taattttagt
     1381 tggagaagat gctatagttg atggattagc atcagcacca cttgcctctc aaaaaaatgc
     1441 accaatatta ttatctaaaa aagattcact accatcagaa atagaagctg aaatattaag
     1501 agtacttgga agtaatctat cttctaagaa aatatatata gtaggtggag aatctaaagt
     1561 atcaaaagaa actgaagaaa aactttctaa attaggtgta agtaaagttg agagagtttc
     1621 tggagaagat agatttgaaa cttctttaga aatagcaaaa caattaaaag atacatttaa
     1681 gactgctttt gtagtaggtg gaaatggaga agctgatgct atgagtatat cagctagagc
     1741 tgctcaattt ggtgctccaa taatagttac aggtaacgaa ttagatgcaa atgctgaaaa
     1801 attattaaaa ggaaaagaat tagaaatagt gggtggagaa aattctgtat caaaagaagt
     1861 tgaagacaaa ttagttgata tagatttaaa taataaagtt gaaagattag ctggagaaaa
     1921 tagaaaagat actaatgcta aagtaatcaa taaatactat gcaggtgcaa ctaaagcata
     1981 tgtagcaaaa gatggttatg taggtagaaa tggacaatta gttgatgcac ttacagcagc
     2041 accacttgca gctagttcaa aggctccaat agtattaact acagaagaac tttctaaatc
     2101 acaagaagaa gtagttgagt taagacttaa gaatgctact aagttagtac aaataggtga
     2161 aggaatagct aaaaacgcta ttgaaaaaat agcagaaaaa ataaacttat ttactaagaa
     2221 ctaataagat ataggaatta atattaaaag tttatttaga taatatatga ttatacaatg
     2281 cgttaatatg taagacatag tgtagatatt ataaaatata caaataacaa gataaaataa
     2341 taaaaataag gtgtctcatt tagaattaga ttgaggtacc ttattttatt ttttgattat
     2401 aatgttgaga aattcatatt gcaaaaatta ttataaaaag atacttctta taatgattaa
     2461 acatct
//
LOCUS       DAFKMX010000307         2456 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.315, whole genome shotgun sequence.
ACCESSION   DAFKMX010000307 DAFKMX010000000
VERSION     DAFKMX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.315"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            290..2074
                     /locus_tag="LZB08_003660"
                     /pseudo
     CDS             290..2074
                     /locus_tag="LZB08_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1074 a          328 c          434 g          620 t
ORIGIN      
        1 attctaaagg ctactttttt tatacaaaac cctatttttt ataacaattc attaacataa
       61 aaatcaaact taccttaaaa tataaattta caaatttttc aatcaataag aaaatttctt
      121 tattccttac ataatgactg caatgtaaca aaataattac aaattaatta tgaaatcatt
      181 atagttagaa acctctttaa aattagatat taagtatcct ttagaattag aacgggaaca
      241 aagttaaaaa attaagtaat atcaaatgga caagggggaa gaaaataaaa tgaaaatacc
      301 aaagaagatt gctgcaatgc ttacagttac aatgatagct ggaagttcaa cagcaggcat
      361 agctagtgca caaactgtgg caacaaattt aacagggcaa gaaagatatg aaactgctgt
      421 taaaataagt caagatggat ggaaaaatgc tgatgaggta gtaatcgtaa acgactcatc
      481 tatagcagat gctctatctg caacaccatt tgcaaaagca aaaaatgctc caatactttt
      541 aacaagtaaa gataaattaa atgataagac gaaagcagaa atacaaagat taaaagctaa
      601 aaaagtatac ttaataggtg gaacttctgt attaagtaca aatatagaaa aagaaataaa
      661 aaatttaaaa atatcttttg agagaatctc tggagttgag agatatcaaa catccttaga
      721 attagcaaag aaattagatg ctataagtga tgttaagaaa atagctgtag ttaatggtga
      781 aaaaggactt gcagatgctg taagtgtagg agcaccagct gcacaaaaca atatgccagt
      841 aatacttgct gattctaaaa atggaacagc agttgcagac aaatttataa aagatgctgg
      901 aataactcaa tcttatgtag taggtggaga gagttcaatt tctgaagctg ttaaaaataa
      961 actaccaaat agtacaagac taggtggaac agatagaaat gatacaaatg caaaagtaat
     1021 aaaagaattt tataaaaaga cagatttaaa aaatgcttat gtaactaaag atggtatgaa
     1081 taaacaagac caacttatag atgcactagc agtaggtgta ttaggtgcta aaaatcaatc
     1141 accagtagtt ttagttggaa agaacttatc agcttcacaa aaatctctag ttaactctaa
     1201 gagttttgat aagataacta aagttggtgg gaatggtaat gagacagcat ttaatgaaat
     1261 gaagtcatta caagaagtaa aaacagttga agctaaaaca atttctgaat taaaatcagc
     1321 tatagataaa gctactgcta atgatgttat aaactttaag ccaactagtg aggttaaaga
     1381 agcatttaca atacaaactg ataaagcagt aacagtaaac ttaaatggta catatacaaa
     1441 gactgtaact atcaacatgc ctaatggaga tgtaaataac tatgcaaaag tggatgatgt
     1501 agttatagat gatgtaaaag atggaacttt tgttaactat ggaaaaataa ctaacttaaa
     1561 ggttaatgat aaaaatggag ccaagataga aaataattct aaaggtgaga ttggtagttt
     1621 aacagtagca tctggagcaa gtcaagtaaa agtgactaat ggtggtaaga taactacagt
     1681 tacaaataat tctaaaggga ctactataga taataaaggt acaataagta gtgttaaggg
     1741 agataattct ccaacaataa gtggaaatag tccaagttct aattcatcag gaggaagttc
     1801 atcaagtgga ggttcttcac atggaggagg ctcatcttca ggaggaagtt cttctaatca
     1861 aacatcagta aataatgaag cagcaaaaat aacaagcgtg cctacaccag caaaagatgc
     1921 aactagatta actatgccat cagtatcaag tggatatact atagcaataa aaacatcaag
     1981 taatgagagt gttataaaaa aagatggaac aataatacct ccaaatacag caacaacagt
     2041 aaaattagta tttacggtaa cacatacatc aagtggtaaa acagcagata caaaagaact
     2101 atctgtaaca gtaccagcta aatcaacaga tgaagaatta gtaaaagcag aggcattaaa
     2161 aataacaagt gtaactgcac cagcaaaaga tgcaactaaa ttaactatgc catcagtatc
     2221 aagtggatat agtatagcaa taaaaacatc aagtgatgag gatgtaataa agacagatgg
     2281 aacaatagtg ccaccagatg cagaaaaaac agtaaaacta gtatttacag taacacatac
     2341 agcaagttca aaaacagcag atacagcaga gatagatgta gtagtacccg ctaagtcaac
     2401 agatgaagaa ttgcaaacag cagtaaataa tgaagcagca aaaataacaa atgtgg
//
LOCUS       DAFKMX010000308         2449 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFKMX010000308 DAFKMX010000000
VERSION     DAFKMX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.316"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            87..1286
                     /locus_tag="LZB08_003661"
     CDS             87..1286
                     /locus_tag="LZB08_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBM7317118.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1338..1841
                     /locus_tag="LZB08_003662"
     CDS             1338..1841
                     /locus_tag="LZB08_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7317119.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            1948..2265
                     /locus_tag="LZB08_003663"
     CDS             1948..2265
                     /locus_tag="LZB08_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317120.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
BASE COUNT         1006 a          167 c          470 g          806 t
ORIGIN      
        1 ttaatacaat taaattaagc ttagtttgaa atttgtatct aaaaagtaaa gtaatctaga
       61 attaagaaaa tggagataga attataatgg aaaagttata ttatacaaat cagtatataa
      121 gagattttac agcagaaata attgaaataa aagaagtaga aaataagtat catgttttat
      181 tagataagac agcttttttt cctggtggag gaggtcagtt tggagacctg ggaaaaatag
      241 agaatgatga tgtaataaat gtgtatgaag atggaaaaaa aatataccat gtattagata
      301 aaaaacctat aaagatacat aaggtaaagt gtagtattga ttgggataga agagaagatg
      361 gaatgcatca acattttgga caacatgtat tgtctggatg tttttttaaa acttttaatg
      421 caaatacgac aggttttcat ttgggaaatg agtatagtac agtagatata gaaggtaatt
      481 tagaagagga taaaattaga gagatagagt tgtttgccaa tgaaattatt cgtcaaaata
      541 tacaagtaga gacattactt ccaacgaaaa aagaactaaa aaagatttgg ttaagacgtg
      601 atttgccaaa tactgatgaa gagataagag taataaaaat aggtgattta gatacgaatg
      661 catgttgtgg agtgcatcca aaatcaactg gtgaattgag aatgataaaa ataaagaaat
      721 gggaaaaaca taagtcaagt acaagaatag agtttctagc aggtaaaagg gcagtggatg
      781 aagtattgaa gagggatata tatttgacta aaatatgtag atatttaagc tgtagtgagg
      841 aagatgctat aaatggtatt gtaaatctaa atagtaaagt ggaagaggct ttaattaaaa
      901 aaagaaggct tgaagaagta gtatctacat atcaggtcaa agaaatgatt gaaaaagcaa
      961 ataaggttgg caatatttcg gtcattaaaa aaatttatga tgatgaagat ttgaaatatg
     1021 taaataagat agcaaataaa attgcagaat atgaaaatag tattgcatta ttagctgtta
     1081 aatttaatga taaaataaat ttagtttttg catgttctaa aaatttaaaa gatattaata
     1141 tgggagtttt attaaaggat gcaataagtc ttatagatgg caagggtggt ggaagtaaag
     1201 tattagctca aggaggaggt aagaataatg gaaatttaga ggctacactt gattatgcat
     1261 ttatgaagat agaaaaaact ctataaatat attatataaa aaaccttagt agataaaata
     1321 gatatggagg aataagtatg gagcaacaaa aggactacat agagttatat gaaaatgaag
     1381 aactaataga gataagagaa gcacgtatgg atgatttgga tacaatagca aaatttaatt
     1441 ataatttggc taaagaaact gaaggaaaag aacttgatat ggatgtttta actaaaggtg
     1501 taaaagcatt gttattagat gagagaaaag gaaaatatca tgtatataca gtatttgata
     1561 aagttgttgc acagattatg tatacatatg agtggagtga ttggagaaat ggaaatttcc
     1621 tttggattca gagtgtatat gtagataagg aatatagaag aaaaggtatc tttaattatt
     1681 tatttaatta tataaaaaat atatgtgata aagatgaaaa tatagttgga atgagattat
     1741 atgtagaaaa agaaaacata aatgcaaaag caacatatga gtctttaaat atgtatgagt
     1801 gtgattataa tatgtatgaa tacgaagtag tacattcgta atataaattt gtaatactgt
     1861 tatactgatg ttataaagta ttgattatat tgacgattat atgaaataaa ctggatatta
     1921 taattagaat ttttaggggg tataaaaatg ggcgttttaa aaggaatgtt ttatgtattt
     1981 ttatttttag atttagtgtc aatattctgt ttctttttca ataaaggaaa aatagcatct
     2041 aataaaattg tgtttaatgc aataggagta cttacatttg tactttgttt tatgcttttt
     2101 agttattatc ctaataataa tcttattgga aaatttattg cttctctatt ttttatattt
     2161 ggagttgcag gagttatgtt gaaagaaaaa aattttttat atgctagact tcttttaact
     2221 gtagttatag ttttttctac attaagattg ttcgttattc aataaaatgt atttgtatga
     2281 aaatttatta aagtctacac atttaagtgt aggctttttt gattttttgt ttgaaaatta
     2341 aggaattttt aaggaaattt taaaatcgtc ttaaatattt attgatatac ttaaaatatt
     2401 aatagcgaaa ggagacttat cagtgataga aagtaaaaag tcagaaaaa
//
LOCUS       DAFKMX010000309         2376 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFKMX010000309 DAFKMX010000000
VERSION     DAFKMX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.317"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            60..1064
                     /locus_tag="LZB08_003664"
     CDS             60..1064
                     /locus_tag="LZB08_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7317121.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLKIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1190..2302
                     /locus_tag="LZB08_003665"
     CDS             1190..2302
                     /locus_tag="LZB08_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBM7317122.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPVNVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
BASE COUNT          969 a          247 c          453 g          707 t
ORIGIN      
        1 taggatgtaa aaataaatag gatataaaaa tatcgtaaat gtatgaggag tgaaatttaa
       61 tgcgtttcaa aagaatattg atattgatgt taactgtagc aatgatagct ggaatgttgg
      121 ttgggtgtgc taataaccca agcaataatc ctagctctag caaagataat aaaagtgata
      181 taagcgataa aagtaattca aaaaaatctg tatcaatgat actagatatt gaaggaacaa
      241 ataatgaagc tatgaataat tcagctttat tagcattaaa caatgctcaa aagaaactta
      301 agatagatac aaacaaagtg gaatcagatg attcctcaac tttttctaat agtatagata
      361 ttttatgtaa tgacaattac gatttaatta tagctgtagg agctagattt gcaaaaccat
      421 tggagatggt agccaagaaa tatccaaagc aacaatttgc aattatagac tatgagtatg
      481 acaagcaacc ttctaatata acttcaattt cttatgaaga taataaatct ggatatctag
      541 ctggattaat tgctggaaaa atgactgaaa gcgataaggt tggttttata ggaggaacaa
      601 agagttctag tagagataaa tttgaatcag gttttagaga gggtgttaaa ttttctaata
      661 gtagtataaa ggatatttca gttgagtatg cagatgtttt taaagatagc aaatcagtag
      721 aatctatagc taaaaaaatg atggataatg gagtggatat tattttttct actactgaag
      781 atgattcaaa agctgtaata gatgcagtaa gggcaaaaaa taaaaaagtt atagcaacga
      841 ataaagacca acatgagtta gctccagaaa atgttataag ttctattgta aaagattttg
      901 agaatcctac ttataatctg atacaaagct ttgtaaaagg aaattataaa ggtggaaaag
      961 ttatagaaaa cacagttaag tctggaagta ctgggcttgc ccaaaatagt tctcaaaata
     1021 ttcctccaga tgtgttagaa tatgttaata agaacaataa ataatctact ataatatcca
     1081 agaatactta tgactttagt tatgaggtga attggatgtt ataatagaca tatatagaaa
     1141 gacatatgca gaggtaggtt agacctatct aaatcaggga gtgaattttg tgaaaataaa
     1201 aaggatattg atggcttgct ttgtagtagt ttttacaatt tttatattag ctatatgtaa
     1261 ttttgatttt gggtttgata aagtaaataa gaataattct aatatagata gtgctaaatc
     1321 gaaacagagt gagtctaaaa gtttgccaac ttcagcagaa aaggataaat caaatagtaa
     1381 aagtaagaat aaagaagatg acacagaaga tgattcatat gtaagaaaaa ctattggagt
     1441 attgttagat accgaaaact taaataacga atcatataat aacaatgtac tatcagcctt
     1501 aaaaagggct gatgaagatt ttgatgttag gataaaactt gaaaaaccaa agacttctaa
     1561 aagttttaaa gagagtatag aaaaactgca taagtatgaa ccagatttga taatagcagt
     1621 tggagccaga tttgcagagc caattaaaga agctgctact aaatatccca aacagcaatt
     1681 tgcaatagtt gactatactt ataaagttca gccagttaat gtaatttctg tatcatttca
     1741 agataatgtt actggatatt tagcaggact tattgcaggt aaaatgactg atactgggaa
     1801 agtcggtttt ataggaggag ttgagggttc tagtagagat aagtatgaat ctggatttag
     1861 aaaggggctt aaaacttcaa ataaagatgc tgagttacta gttaagtatt caaatatatt
     1921 tgaaaaccct gaatcagcta gaactattgc aaaggaaatg agagattctg atagagatgt
     1981 tgtattttca gctacagaag atgatagtaa aagagctatt caggtagcaa gagaaaatgg
     2041 tggtaaagtt atagctatta ataaagacca atacgatatg gctccagata atgtaataag
     2101 ttcaattata aaagatgtag aacaacctgt ctatgaactg ataaagaatg ttgcaaaaga
     2161 tggttttaag ggaagtaaag taatggaatt taaaataaaa gatggagaac ttggcttgac
     2221 ttcaaagagc agtagaaata taccaccaga tgttttggaa tacataaaaa aagagtataa
     2281 caaagtgaaa gatacatatt aattattgat gtagaagatt ataaaaatat actgcttaag
     2341 tttagattag caataaaaag atatatttat taaatt
//
LOCUS       DAFKMX010000310         2368 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFKMX010000310 DAFKMX010000000
VERSION     DAFKMX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.318"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..390
                     /locus_tag="LZB08_003666"
     CDS             <1..390
                     /locus_tag="LZB08_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7317123.1"
                     /translation="LNELENDVYTDSKCMSYVLDQIINNAIKYSKKVGKIEFNSKKLE
                     NGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEI
                     EVCSKIGSYTIFNIYFYDISDYLSLDS"
     gene            821..1981
                     /locus_tag="LZB08_003667"
     CDS             821..1981
                     /locus_tag="LZB08_003667"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBM7317124.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVAVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            1983..>2368
                     /locus_tag="LZB08_003668"
     CDS             1983..>2368
                     /locus_tag="LZB08_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317125.1"
                     /translation="MSNNKKKKRKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNI
                     PTISFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVET
                     KRGFSLGFFRLYILFALSFAIIGYFW"
BASE COUNT          938 a          221 c          412 g          797 t
ORIGIN      
        1 ttgaatgaat tagaaaatga tgtttacaca gattctaaat gcatgtcata tgttttagac
       61 caaattatta ataatgctat aaagtattca aagaaagttg gtaagattga atttaattca
      121 aaaaagttag aaaatggagt ggtattatct ataaaagatt ttggtatagg tataaatgag
      181 gaagatatat ctagggtttt tgacaaggga tttacgggga aaaatggaag aaatcaattg
      241 tataagtcta ctggaatggg gatgtatttt gtaaagaaaa tgatagatag tttagggcat
      301 gagattgagg tatgttcgaa aattggttca tatacaattt ttaatatcta cttttatgat
      361 atatcggatt atttaagcct agatagttag gttattagac tataaaatct atcatcatat
      421 acaaatcgac atatacaata agaataaatt tagttcttga aaatagtgaa gatttaatta
      481 gtgccttaaa tgatgctcta tctatattat aaaataataa ttattatata tgaaaaacat
      541 atcagatagg tacctggtat gttttttatt tgtagtaact attttaatat ggtttttgga
      601 gagagaatat gttttgtttg tgaaaaaaaa ttacatggta ttacattgac actgaaaata
      661 aattaatgta agatttagct aaggtaaaaa acgtgtataa gattaactca attaatttca
      721 taagagtaaa ttatcgtaat taaagatgaa ataagattaa ttctaaaatt attaaactta
      781 ataccttgat ttggggagga ataactataa acttaaaagg atgataacta tgtatagtgt
      841 aggtttaatg tcaggaactt ccttagatgg agttgatgca gtactagctg aaattagtgg
      901 gaatggtaga aatactaaag ttaagcaaat agaatttata actctagaaa ttcctaaaga
      961 tataaaagac gaaataagaa agtgctgtat agaagaggaa tcgagtgttg atttaatctg
     1021 tagcttaaat tttaagcttg gctatttatt tagtaaagct gtaaaatcag tgtgtcataa
     1081 agcaaacttt catatagcaa atttggattt tatcgcatct catggtcaaa caatctttca
     1141 tataccaaga agttataata attttgtatc ttcaacgctt caaataggtg aaccagcagt
     1201 tatagcatat gaaacaaata caaaggtaat atctaatttt agagtaatgg atatagcagc
     1261 aggtggagaa ggtgctccat tagtaccata tagtgaattt ttattatata gtgataacaa
     1321 taaaaattta gcacttcaaa atattggtgg aataggaaat attactataa tacctaaatc
     1381 atgtaacata gatgatgtat ttgcatttga tacaggacca ggaaatatga taatagatgg
     1441 agtatgtcaa agattattta atagaaagta tgataaaaat ggatactttg catcaaaagg
     1501 taaaattaat gaagaaatgt taaaagattt gatgagtcat aagtatctta gtcaagcgcc
     1561 acctaaaagc actggaagag aggtatttgg acaagtttat ttggataaca tgcttaacaa
     1621 atataaacat gtagataaat atgatttgat agcaacagta actatgttta cggcaaaagc
     1681 tatatattat aattataaaa acttcatact tccaaaggtt aatgtagata ctttattaat
     1741 tggaggtgga ggagctcata atttaacttt aataggatat ataaaagagt tattattaga
     1801 ggttgcagta ttaactcaag atgaatatgg gtattcttct gatgcaaagg aggctttggc
     1861 atttgtaatc ttgggcaatg aaacattaaa taattctttt tctaatgtaa tatctgcaac
     1921 aggggctaaa aataaagtta ttttagggaa tataacacct aaaccttttg gaggtaaatg
     1981 acatgtctaa taataaaaaa aagaaaagaa agaaaagaaa gaaaaataat aattataaaa
     2041 atgcaaaaag tacaaaaagt aataaaaata ctaagaaaca aatggagaaa aatcaaagaa
     2101 cagattataa tattccaact ataagtttta aaagtggatt aatactaata gtttcatctc
     2161 tagtaagtac tgtagttttt cctttgttat tatctatatt tggaatagat aatagatttg
     2221 gagttgttat aggaaattca ttaattacat cttttgcagt tgcatataca agatactttg
     2281 ttgaaactaa gagaggtttt tctttgggat tttttagact gtatatttta tttgctttat
     2341 catttgcaat aataggttac ttctggat
//
LOCUS       DAFKMX010000311         2365 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.319, whole genome shotgun sequence.
ACCESSION   DAFKMX010000311 DAFKMX010000000
VERSION     DAFKMX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.319"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(78..2060)
                     /gene="bclA1"
                     /locus_tag="LZB08_003669"
     CDS             complement(78..2060)
                     /gene="bclA1"
                     /locus_tag="LZB08_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBM7317126.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATGVTG
                     EIGPTGVTGPTGEIGPTGATGVTGSIGPTGATGVTGEIGATGPTGVTGSIGPTGATGP
                     TGATGEIGPTGATGPTGATGPTGNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGPT
                     GATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVDANGTFTVQENGVY
                     YISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTT
                     FTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          538 a          530 c          377 g          920 t
ORIGIN      
        1 gtaaaactca atatataaaa atacttttgt catttaaata aagcctaaat agtttttaag
       61 attattttag acacgtttta agtcataaca gtatcagcta ttctaaatat cgtcaaagta
      121 gccgcagttc ctgctgattg cccatttcgt atccctactg ttgtagatga aaaattattt
      181 attgtaaaag tagtcccacc aaataaaaaa ccagcaaaat atccagaaag tactcctccg
      241 cctcctggct cagtagtaag ccctgccaaa tcttttcctc ctaaaattgg gaataggata
      301 gcgaaagaat attgatttat acttgatgaa cctggttgta atgctactga aactgatata
      361 taatacacac cattttcttg tactgtaaac gtaccatttg catctactgt tatacttgaa
      421 ctatttccta ttattgtttg ccaacctgtt atagcatctc cattatttag tgtttgtgga
      481 gatggtgcat ttacgaggaa gttagcaact ggctgagagg aattgcctgt tgctcctgtt
      541 ggacctgttg gacctgttgg gcctgttact cctgtcgctc ctgttgctcc tgttggacct
      601 gttactcccg ttggccctat acttcctgtc gctcctgtat ttcctgttgg gcctgttgct
      661 cctgttggtc ctgttgctcc cgttggtcct atttctcctg ttgctcctgt tgggcctgtt
      721 gctcccgttg ggcctatact tcctgtcact cctgttgggc ctgttgctcc tatttctcct
      781 gtcactcctg tcgctcctgt tggtcctata cttcctgtca ctcctgttgc tcctgttggt
      841 cctatttctc ctgttgggcc tgttactcct gttggcccta tttctcctgt cactcctgtc
      901 gctcctgttg ggcctgttgc tcccgttggt cctatttctc ctgttgggcc tgttgctcct
      961 gttggcccta tttctcctgt cactcctgtc gctcctgttg gacctgttgc tcctgttact
     1021 cctgtatttc ctgttggacc tgttgctcct gttactcctg tatttcctgt tggacctgtt
     1081 gctcccgttg gtcctatttc tcccgtcact cctgtcgctc ctgttggacc tgttgctcct
     1141 gttggtccta tacttcctgt cactcctgtt gggcctgtta ctcccgttgg gcctatttct
     1201 cctgtcactc ctgttgggcc tgttgctccc gttgggccta tacttcctgt cactcccgta
     1261 tttcctgtcg gacctgttac tcctgttggt cctatacttc ctgtcgctcc tgtacttccg
     1321 gttgggcctg ttactcccgt tggccctata cttcctgttg ctcctgtatt tccggttggg
     1381 cctgttggtc ctatgcttcc tgtcgctcct gtacttccgg ttgggcctgt tactcctgtt
     1441 gggcctgtac ttcctgtcgc tcctcttggt cccattggac ctgtaattct aattggaaga
     1501 cactcacatt taggataaca attatctata attctatctt caaatgttac aagtatatac
     1561 ggtttgttac aacctctact agatgtaaat tcaagaatct gataagggta atttaaagat
     1621 aaagctaacc cataattagg atatttattc attgcccatg cttttatagt tcccgtaata
     1681 tttaaacaaa tatagttgga tgtatcactt atcccaattg gtaaatatcc cctgatattt
     1741 tccatagaca ctctatcatt ccatgttata gttcgtaaat tataatattc acttattctt
     1801 ttaatttcaa tatctacttt ttctttaaaa atatgattct tatttgaatc aatatgaata
     1861 aataactctg cttttaatat acttttgtct tttaatatat ctggtattct cactttaaaa
     1921 aaagtaatca acttttcttt tacaaaacta tttgaacatt tacttcctat taagcaataa
     1981 tctaaattac taaagttttt atctggatat tttttagata taaaagtatc atcatttaaa
     2041 taaagtataa tatttctcat ttataatccc tccattttgg gtctatatca tactatgttt
     2101 aaaatacatg cttagttaca atataattaa aatttatagt attaaatttt tatataataa
     2161 tgctgatttc tacaaaaaaa gaacatgtca ttagaaattt ctaatagcat gttctttttc
     2221 attattatat attacacttt aaattattcc ttgactggct tagctggtgt aaactaaacc
     2281 agtctttcta aaatattaag cacttttttc ttattatgtt ttttaggatt tattgtctat
     2341 attattgtaa accaaagtta taaaa
//
LOCUS       DAFKMX010000312         2297 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.320, whole genome shotgun sequence.
ACCESSION   DAFKMX010000312 DAFKMX010000000
VERSION     DAFKMX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.320"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..842
                     /locus_tag="LZB08_003670"
     CDS             <1..842
                     /locus_tag="LZB08_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBM7317127.1"
                     /translation="LNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLD
                     KLKELDKDNPVFEKNEEEKEDTSKNEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSK
                     KRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDL
                     YYQALNLKKNKEYEKAIDNFKSIVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYK
                     KYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEIL
                     SEK"
     gene            1024..1395
                     /locus_tag="LZB08_003671"
     CDS             1024..1395
                     /locus_tag="LZB08_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBM7317128.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            1388..2077
                     /locus_tag="LZB08_003672"
     CDS             1388..2077
                     /locus_tag="LZB08_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBM7317129.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
BASE COUNT          980 a          214 c          363 g          740 t
ORIGIN      
        1 aattaaatta tgagtcaata catattttag agaacataat tgaagaagaa cctgaactaa
       61 tagagccata tgtaatttta tctttgttat atgataaact aggtaatgta aaaaagagag
      121 aaggatattt agacaaactt aaagagctag ataaagataa tcctgtattt gaaaaaaatg
      181 aagaagaaaa agaagataca agtaaaaatg aagaaataaa aaaagtaaaa agaaagaaaa
      241 gtattttacc atatattata gttggctgtg ttttaatagg tatggggata tatttgattc
      301 aaagcaaaaa gagaatagaa aacttgaata accaaataag tagtaaagag gaaaagttaa
      361 gtgaaactga taaaaagtta ggagaaacta gtaaaaaatt agaaaaaacg aataaggaat
      421 taaatgaggt taaaaataaa gaaccagaaa gagagataac tgtagctgat gaggaagatt
      481 tatattatca agctctaaat ttaaagaaaa acaaagaata tgaaaaagcc atagataatt
      541 ttaaaagtat agtttcttct ggtaaaacta aaaaatatat ttctgagtca atatatcaat
      601 tagcaataac aaataaatta ttaggaaata aggatgaagc tataaaatat tacaaaaaat
      661 atataaatac ttatactaaa aatgaccagt attatgatga ttcatattac gagttgggta
      721 tgctttatta tgataatggc gatttaaaaa atgcacaaca gactttttat agtttgagaa
      781 gtgaagttcc agacagtatg tataacaatt caaaaataaa agaaatttta agtgaaaaat
      841 aggtttatat agttaagttt tttgaaacaa ccaagtatgt attttattaa attaaaataa
      901 aaataaattt aaaaagagat tgtgtcaaat tataactttc tattttaaag aaagatattt
      961 gcttgttgat aaaactacaa atatatgatt tgaagcaatc tctttaattt ttggaggtgg
     1021 aaaatgaaag ttttcaatca attagtaata attttatcta tctgggtaat aggagagtat
     1081 ataagttcct ttatacaagg aattatactt attcctggga gtatagttgg tatgctatta
     1141 ttatttctac tactacaatt taaagtttta gacttgagta gtatagaaaa tgtaagtgga
     1201 tttttcctcg ataatatggc tatatttttt attccagcag gtgtatcact tataaagtca
     1261 ttagacctaa taagggaaaa tgtattagtg cttttattag tcatatgttt aagtacacta
     1321 atagtaatgt atactactgg aattattgtg gagaaaatga taaaaaagaa ggaagaagga
     1381 caaaaaaatg tataatgtat tacagacacc agtatttggt ataatagtaa caatagtttt
     1441 tttcaattta ggaagataca ttcaacgaaa aacaagtaat cctatatgta accctttatt
     1501 aattgcaatc gtaggaataa tattgttttt aagcatatct aaaatccctt atgagaatta
     1561 caagatagga gctgatagtc taaatttctt tttaggtcct gtcacagtag tacttgcagt
     1621 tccactttat aaacaatttg aattatttaa aaagcatatg tttgagatta ttgtaggaat
     1681 aggttgtggt atagtgattt cttttgtatc tgtattgata attggcaaaa tagctaattc
     1741 agatgtaagt ataataaatt cactaattcc aaaatctata acaacaccaa tgggaatatc
     1801 tttagcaaat tctttaaatg gtatagaatc aataacagtt gtagccataa tttttacagg
     1861 tatttttggt ggtatggttg cttctacagt attcaaactg gggaatataa atcatccagt
     1921 tgctaaagga attgctcttg gaacatctgc acatgcttta ggaactacaa aggcttttga
     1981 acttggagaa atagaaggag ctatgagtgg tgtttctatt ggagtttctg gtacaattac
     2041 agtaatacta attccaatta taatgaattt tatataaaaa ataaaattaa gatgagtacc
     2101 ttgaatactt aaaacaaaat aaaataaaaa aaatttaaaa taaaaactga acgaaaaaaa
     2161 tgcaaataat gaaataaaaa aatcatgtac tgaaattata aaaagaaaaa tttcatttag
     2221 gaattaatta taaagagaca attactaaac ttgaatttgc taaaataaaa taaattactg
     2281 cgaaaataga tatttag
//
LOCUS       DAFKMX010000313         2189 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFKMX010000313 DAFKMX010000000
VERSION     DAFKMX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.321"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(40..954)
                     /locus_tag="LZB08_003673"
     CDS             complement(40..954)
                     /locus_tag="LZB08_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBM7317130.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1786..1968)
                     /locus_tag="LZB08_003674"
     CDS             complement(1786..1968)
                     /locus_tag="LZB08_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317131.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          797 a          274 c          275 g          843 t
ORIGIN      
        1 atctaaatat gcaaagatta ttttaaaagt aatttttaat tacatgtttt tataactctc
       61 caatattcta atataatgat ttgcctctct taaaacatgg tctgctaaaa gtggtaatat
      121 tatagatttt atcttacact gttctattcc tgatgctcct gcttctttaa aatctttaaa
      181 ctcaacagtt tcatttagag tttcttctgt aatgttcttg atattagaat cagtcatttc
      241 gtttgttttt tcaattaatt cattaaattt tatagcaaaa tcatttgaag tatttattag
      301 ttcaccttct gaggggtcta gtaatcctct cataaacaga gcatgttcca tcataatatg
      361 gtcccagaat aattctattt ctttagcgtt ttttgactca atatctattt tattttcaag
      421 gtcaactaca taagaacgat ataaatttgc ttcatgtatt atatgttcaa gaagtagagg
      481 gtagtttgat gtaaatatag tacatgatag tacaccatct aagactcttt ctttaaaatt
      541 aataagccca tcaagtaatc ttattgcatc agagttaagt tgagctacat agttcactaa
      601 atcttgacca acttgtgggt ttacaccact ttgtagattt aattctcttg tagtgatgtt
      661 tttgtttatt tctatccctg taaactcttc tgttttttgt tctgcaactg atgtgagagt
      721 agttacaagc tcttctgaat ataatatatc aggtctaatt ataccattac tagcactaac
      781 agtgtatgat aataaatctt caaattgctt tttatagtgg tcagcttcca tagcaagatt
      841 ataattttta tttgtgaatc ctgcttctaa aaaaagagca tgctctttca taattcttga
      901 aaaaaataaa tgtaattcta gtgataaaat aacatatttt tgattatcta tcataatttc
      961 ctccctttag aaagtatcct atttataata ctttattcgt aatatttaaa tttatgtata
     1021 ttataaataa attaactaaa ttcaattgct actaatatca acagttttta ttaagttatg
     1081 ggcgaaaaaa ttatcttact gtgtttctta ttagatatag atataatgat ttaaggtact
     1141 tgttggctat tatttattaa gagtacaagt aatactagaa atttttatga gaattatgta
     1201 atttataagt attattttga tacaaaacaa atgttttttg taaaacaaga acttttatag
     1261 taaaaaatga ataaatatta agcaagcata attttatacc acaacaatta ttaattctaa
     1321 tagctgcact taatgtttta ggtatgggat ttaaaaagta taaaaaatta agtgataaat
     1381 atattccatt atattgcttg tgcttggagt agtattttct gtatagatgt taggatttaa
     1441 tccaacttct attttacagg ggattctatg ctggggtgtt tcaattggat taaattaagt
     1501 atgcaaacaa tttcaagaca atgataaata attggatgtg tgataggaat atcaacaaaa
     1561 aacattatta aaaagaatta ttttatttat aaaataaaac aagtaataag aataaataag
     1621 gagcaactaa aatgatttaa atatactaga attaaaaaga gaatgtaaag ttgtctagta
     1681 atggatgtaa aatattttaa acgataagct atgaaattga tatattaaaa tgggtaagaa
     1741 gaatattata taggattctt cttacccatt ttaataaatt taattttatg ccaaaccaca
     1801 ttcgcatagt agaatcatca ctctatggag acataactct acaacatctt ctccatattt
     1861 tttaataaaa tcaccaattt gagtttctat gaatgaatag tattctttta aactagcaat
     1921 atatgcagtc attatatgaa cacattcatc aatcttatct ttaaacacaa ctaatccccc
     1981 taaaaaacga aaaataaaaa tattcagtaa atagcagata ttatttcact cataaatcta
     2041 tatagtatga tatattgctt agatatattt tacaagtgga tatgtaaaac tggttattat
     2101 aatcgcaaaa ctggataaac tttcattctt aaaagaaaat ttatccagtt taaagtttaa
     2161 ttatgaatat ttgagttagt agatattta
//
LOCUS       DAFKMX010000314         2156 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFKMX010000314 DAFKMX010000000
VERSION     DAFKMX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.322"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            1127..1516
                     /locus_tag="LZB08_003675"
     CDS             1127..1516
                     /locus_tag="LZB08_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBM7317132.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          980 a          113 c          380 g          683 t
ORIGIN      
        1 tgaaaaaatt taaactttgt aatatataaa taaaaaaact ctaaaataga aggatataat
       61 tatatagaat gtttcaatta aacaaggtaa aaactataat cttaaaatta aaattaacat
      121 tattcttaag aagcaagaaa tttagaaagt aggaagttta gtgataagtg gtagtaagta
      181 agaaatttag taagtgggaa gtttagtagt aataatttat agcaagtaag aaatttagga
      241 agtaggaagt ttagtaataa tttgtagcaa gtaagaaact tagggagtag gaagtttagt
      301 aataatttat agcaagtaag aaatttagga agtaggaagt ttagtaataa tttgtagcaa
      361 gtaagaaact tagggagtag gaagtttagt aataatttat agcaagtaag aaatttaggg
      421 agtaggaagt ttagtaataa tttgtagtaa gtaagaaatt tagaaagtag gaaatttagt
      481 aataatttat agtaagtaag aaaattaggg agtaggaagt ttagtaataa tttatagtaa
      541 gtaagaaatt tagaaagtag gaagtttagt aataatttat agcaagtaag aaatttaggg
      601 agtaggaagt ttagtaataa tttgtagcaa gtaagaaatt tagaaagtag gaagtttagt
      661 agtaatttat agtaagtaag aaatttagta agtgagaagt ttagttataa tttatagtaa
      721 gtaagaaatt tagtaagtga gaagttcagt aataagttgt agtaaataaa aagtttagga
      781 agtaggaagt tcagtaataa gttgtagtaa ataaaaaatt taggaagtag gatttactga
      841 aatacaaata tttaaaatca aagagtttaa aagaaaggag agctactgga caagtatcca
      901 aaatagatag ctgagtacaa aatatttcat aaaattaagc aatataatac aagagaattt
      961 tgacattatt tatatatatt taccgtgtac aaatagctaa atttaggatt aagattgaaa
     1021 aatatcttat aagtctgagc ttatataaat ccgtataaga tgtatttaat cgaaaaattt
     1081 tattatggat attgcttgtc tatattctga gtagcaaggt tttatcatga ctataaaaaa
     1141 gttgccacaa tcagaattaa agataatgaa atttatatgg aaatcagatt ccaaagtaac
     1201 atctagagat attgttttag gaatggagca aaaatatcaa tggaaacaaa cgacaacttt
     1261 gactctttta tctagattag tagtaaaaag atttctaaat tctcaaaaga tagataaata
     1321 tacgcattat gaagttttaa ttaaagaaaa ggaatacata ggagttgaga caagagattt
     1381 ctttagaaat attcatgatt cttctataaa aagtttattg ttgtcattac atgaaaatat
     1441 taatttgagc aaagatgata ttttacttat tgaagagtgg ataaaaaatc taaaagaaga
     1501 ggagaaagac gtataagcaa acgtatttct tatagcatca atataaagtg tatgtaagtg
     1561 tatttatgta atatattagt ctacttaagt aatatgacaa atgattataa gtggattatg
     1621 aaactataat gtgaatctaa ataaattaaa tataaattaa tagtggatat tctatttaat
     1681 taaatttaaa aagctacctt tacttataag tataattaag gtagcttttt aaatagaggt
     1741 agtgtaatta aagagaattt ttaaatataa attgtgaagc tttaatatag atgataatat
     1801 atattttgca aatatgacac agtgttttcg tatttaagat gataaaataa ataggaatta
     1861 atgatgatat tgaaaaaata aaaataaagg attaatataa gatattaaag atatagagat
     1921 aaaagtgtta acagaagata ttgaaaaaat aaaagattga tataagatac tgaagaagta
     1981 gaaataaaag tattaataga agatattgaa aaaataaaaa caaaagattg atagaaagta
     2041 ctgaatagat agagataaaa gtgttaatgg aagatattga aaaaataaaa gattgatata
     2101 agatactgaa gaggtagaaa caaaagtatt aatagaagat attgaaaaaa taaaaa
//
LOCUS       DAFKMX010000315         2128 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.323, whole genome shotgun sequence.
ACCESSION   DAFKMX010000315 DAFKMX010000000
VERSION     DAFKMX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.323"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            164..1477
                     /locus_tag="LZB08_003676"
     CDS             164..1477
                     /locus_tag="LZB08_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBM7317133.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1495..2031
                     /locus_tag="LZB08_003677"
     CDS             1495..2031
                     /locus_tag="LZB08_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBM7317134.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          837 a          203 c          408 g          680 t
ORIGIN      
        1 atatagttta taatacaaaa gtataattac attataatta tatgtagtta gttggatttg
       61 tgtatttaaa taatgtataa tttatgctga ttactgctaa aaaagcataa taatattgca
      121 ttgtaaggtt ttataaactt ctaattgaaa ggggataatt caaatggata agagatattt
      181 agaatccgat gtatttgaag cttttcaaga aagactttct tatcttttta atgaatttga
      241 ttatatctat gtatctttta gcggaggaaa agatagtggt ttactgctaa atttagtttt
      301 ggattataag agaaagaata atataagttc caaaatagga gtatttcatc aagattttga
      361 ggctcaatat agtttgacta ctgaatatgt tgaaaaagta tttgaagata atattgaaga
      421 tattgaacca tattgggtgt gtttaccaat ggctacaaga actgctgtag gaaattatga
      481 aatgtattgg tatccatggg atgatgagaa agaagagtta tgggtaaggg aaatgccagt
      541 caaagaatac ataataaatc aaaaaaataa tccttttact tattataaat ataaaatgag
      601 acaagaaagt ttggcaaaac aatttggtag atggattaga gatatacatg aaggtgcaaa
      661 agttgtttgc ttattaggaa cacgagcagc agaatctcta caacgttatt gttctattgt
      721 gaataaacag catggatata aaggaaagtg ctggataact aaggaattta aaaatgtctg
      781 gacggcatct cctatatatg attgggaaat agaagatata tggttagcac actataaatt
      841 tgactatgac tataataaaa tttatgactt attctataaa gctggagtag ctccaagaaa
      901 aatgagagtg gcatcacctt ttaatgattc agcaaaagag agtttaaatt tatacagaat
      961 attggaccca gatgtatggg tgaaattggt tgctagagta caaggagtta attttacttc
     1021 tatttatggc aaaacaaaag ctatgggata tcgtagtgtt aaattgccat atggacacac
     1081 atggaaaagt ttcacatatt ttttgcttga taccttacct gaaaatataa ggaatagcta
     1141 tattaaaaaa ttttcaacat ctattgagtt ttggcagaat acaggtggtg gtttgtcaga
     1201 aatagttata cgtgaattgt tgagaaaagg ttataaaata gaattaaatg gagtttcaaa
     1261 tcatacaaaa gataaaaaga gtagagtcat atttttggat gatatacctg atgatacgga
     1321 tgatattaaa tttaccaaag atataccaag ttggaaaaga atgtgttatt gtattttaaa
     1381 aaatgaccat atgtgtagat ttatgggatt tggtcctaca aaagaacaga gaattagaac
     1441 ggaatatatt aagaagaaat atgaaaaaat tcattagata ggagttaaga tgctatggaa
     1501 tataaaagtc ctgtatatgg aatcatcgca gtgccaatcg aaaagataga atctaacgac
     1561 tataatccaa atgcagtagc cccaccagaa atgcagttgt tgtataaaag tataaaagaa
     1621 gatgggtata caatgccaat tgtctgttat tataatgagg aaaaggataa gtatattatt
     1681 gtagatggtt ttcatagata tagagtaatg cttgaaaatg aggatatata tgatagagaa
     1741 aatggacttc ttcctgtttc agtaattgat aaatctcttg atgaaagaat ggcaagtact
     1801 attcgacaca atcgtgcaag aggttctcat aatgtggatt taatgagtaa aattataact
     1861 gaactttgtg aaattggaaa atctgatgaa tggatatcaa agcacctggg tatggaagtt
     1921 gatgaaattc ttagattgaa gcaaataact ggattagctt atttatttaa agataaagag
     1981 tttggtaatg catggattcc ctttggctct gatatagaag aagaaaatta aatagattta
     2041 ttaaaagaaa aagatttatt aaaaaagaaa atttaaaata tatattaaaa aataaagagt
     2101 tttaatatta tatgtaagta cagaaaaa
//
LOCUS       DAFKMX010000316         2105 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFKMX010000316 DAFKMX010000000
VERSION     DAFKMX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.324"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(84..635)
                     /locus_tag="LZB08_003678"
     CDS             complement(84..635)
                     /locus_tag="LZB08_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317135.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            826..1788
                     /locus_tag="LZB08_003679"
     CDS             826..1788
                     /locus_tag="LZB08_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBM7317136.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          787 a          234 c          366 g          718 t
ORIGIN      
        1 aaataaaata aaaaagataa atcctaatca aaggatttat ctttttgtat agaaaataat
       61 tattctatta ttaaatggca tggctaaact ggataataat ttattattac tgctttcaat
      121 gttttttctc cttgattatg gtatgtatga attgattcag ctgaaaaaca aatagagtca
      181 ttttctctaa gttgataaat agagtcattt acttctaatt ttaattgacc ttctataatc
      241 ataatatatt cctgtgattt ttcagaatga ccaacagatg tataactatg accttcttct
      301 aattccattt gaaataattc gaagtttcta gttggtgtat tgggataata acaataaaga
      361 cgataatctt tattttctga aacttgtaca tctatatcag tttttgatac aataaaagtt
      421 tcattttgag gttgttccag tattgcagta tatggaacat ttagtccact tgcaattttc
      481 caaattgtgt ttactgttgg gtttgagtct cccttttcaa tttgagaaag cattacttta
      541 cttacgccag ataattcagc aagttgacct agacttaagt tacgttcatt acgtaatcta
      601 tttaaatttt tagcaataac agtattaata ctcatagtag ctccttaaaa tgtaaattat
      661 tgacaaatat aatttacgaa tgtataattt atttaacatt atttataatt tacaaatgta
      721 taatataatg aacgttaaat acactgtaca tccgtaatat aaattatact gcataaagag
      781 ttgtttttca acggatgata gttgagagat taagggggaa ttgtaatgcg tgtacttaat
      841 aatgttacag aggctatagg caatactcat atgttacatt tatccaaaat agcagatgaa
      901 tttggagttc aaggtaatat ttatgcaaag atggaatact taaatccggg atttagtaaa
      961 aaggaccgtg tagcattaca aatgattacc gaagcagaaa atgcaggttt attagttcca
     1021 ggtcaagcag ttgtggaatt aacaagtggt aatactggaa caggactagc aatagcttgt
     1081 gcatgtaaag gatataaatt tatagcgtgt atgtcaaaag gaaattctat ggaaagagca
     1141 agaatgatga aagctttagg tgctgaagta gttcttgtag accaaatgcc taattcagtt
     1201 catggacagg tatctggtga agatttggca cttgtagaaa ttgaggctca aaaaatagca
     1261 aaagaaagaa atgcatttag agcagaccaa tttaatttag aagcttgtaa tcatgcacat
     1321 gaaaagtata cagcagaaga aatgtgggaa caattggatg gaaatattga tgtatttgta
     1381 gattttcttg gaagtggtag tacttttgca ggatgttcaa aaacacttaa aaaacataat
     1441 tcttctatca aatgttatgt tgttgaacct gaaacagcag caatttattc aggtaaagaa
     1501 ataactgacc aagggcataa aatacaaggt ggaggatatt ctatggattt gccattagta
     1561 gacaagaaac ttattgatgg ttatattcaa gttacagacg aggaggcaac tgatgtagca
     1621 agaaaattag ctaaattaga aggtgtattt gcaggatttt cttcaggggc aaatgtatgt
     1681 gcagcaatta agttattaaa gagcagtgaa aaaggaaaaa atatagtttt aactcttaat
     1741 gatagtggtt taaaatattt aagtactgat ttatatgaag aattgtaata atagcacaat
     1801 agttatgcaa tgttttttaa attaagtata tagatttaaa ttataaaaag tatctcaatg
     1861 taatttttga aagttataaa ggagatactt ttttattttg taaatatgga gtatcctact
     1921 tataagtatt aagctatata gatattgatg attagctatg caataaaaaa gagtatgtgt
     1981 ttttgcattt atacatcata acttggaata atacatatta atgttctaaa tgtatttaaa
     2041 atatatgata aaattagaac aaatagatac aaggagataa aaatgcaaaa gagaatttta
     2101 agaaa
//
LOCUS       DAFKMX010000317         2069 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFKMX010000317 DAFKMX010000000
VERSION     DAFKMX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.325"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            379..672
                     /locus_tag="LZB08_003680"
     CDS             379..672
                     /locus_tag="LZB08_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBM7317137.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            653..1294
                     /locus_tag="LZB08_003681"
     CDS             653..1294
                     /locus_tag="LZB08_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBM7317138.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1538..1870
                     /locus_tag="LZB08_003682"
     CDS             1538..1870
                     /locus_tag="LZB08_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBM7317139.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          790 a          187 c          318 g          774 t
ORIGIN      
        1 agtagatgtt tttatgtact tttatacagt atatttctcg tttcattctt ttctatttaa
       61 ataggactta actacttttt aaattgggtt tagctacttt taatttaaaa ttaaaagtat
      121 tgttatatat ataagtacct cagtcattag gtttttgctt taacaattat ggtgcaatac
      181 acttttgtta agttaaaaaa attagataaa gtaagattaa ataaaagcga cttaagtaaa
      241 ttgagaatct taatatgaaa attttcaata aatatttaga aataactatt tagaaaaatt
      301 tctaaatagc tattgaaata aaatggatgt tatgttatat tctatttaga tgaaattcta
      361 aatagcgagg tgatggtgtt ggggtttcca gaaacattca aagctctttc agaccctata
      421 agaagagaaa tattggttat gttgagggag aataaaatgt cagcaggaga aattagtgaa
      481 aaatttaata tgacgaatgc tacaatttct tatcatctat ctcaactaaa aaaggcagga
      541 ctcatatttg aaacaaagta taagaacttt attttctacg aaataaatat ttcagttttc
      601 gaagaggtca tgctttggtt ttcacaattt atggagaagg gagaaaaaga taatgaaaaa
      661 agcgataaat aaacaattta ttttatctac tttaatatgt tttataccat ttattgtatc
      721 aatatatttt tataatagat taccaaatga agttgcaatt cattttgata attatggaaa
      781 tccagataat tatgctccaa aggttatagc agcttttggt gtaccattgt taatgctatg
      841 tatccatttg tatacatggt ttagacttga gaatgagcct aagaaagcta atttttcaaa
      901 tgttatacaa aatttatcaa aatggggagt tgctatactt tcagtaatta tacaaataat
      961 gatggtttta tattcaacag gagttagttt ggagtctaat ttttatacag gattaatttt
     1021 aggaataata gttattttat ttggaaacta tcttcctaaa tgtaaacgaa attactcagt
     1081 aggaataatt ttgccatgga ctctgaatga tgaagataat tggaataaga cacaccattt
     1141 agctggatgg atttggctta ttggtggaat attattaatt attaatgcat ttattaatat
     1201 tcctttttat aatatctttg taatctttgt aattgtaata ctaccattta tatattctta
     1261 tttgatgtat aaaatttcag gtaaaaataa ctaatataat taatcactat aataatgttt
     1321 ggataagaat aatatatttc agaactgaga tagataataa agaaattact aagatttcta
     1381 ttttagttct gaaaaaatct tacttatatg ataacttatt gagatttcaa aaaataatat
     1441 aaattaggct aatgaacata aaatgaaaat ttgtatttaa tattgaaaat ataagtacag
     1501 tgtgatattc ttataagaaa aacataggag gtagaagatg aatactagag aaatgaaaga
     1561 atatagggta ttttacaata aaaaattaag cgaaacagat gagtttttta gagtatttgg
     1621 aagtcttgat gataagactt attcagaagg ggctataagt aaaaaaaata aggaattaat
     1681 gggactagct atttcaattt taagccgatg tgatgagtgt atttgttatc atattgaggg
     1741 ttgtattaat tctggagcaa gtaaagatga aattatagaa gctataaaaa taggtgtaat
     1801 tggtggcggg tctattacat atccaaacgc aagatttgca ataaaaattt tagaagaatt
     1861 taatatataa ttttgataga cgtagtttat ttagactaat aataatagtc tatttaagtc
     1921 tttgaaatta agctttattt tctaagttca cttcttatta agtttttaga attgcatata
     1981 taaatataaa tttttaaata atatagtagt tttaaattat ttacatcata gattgtattg
     2041 agaatagtat attgtcatta ttaaatctt
//
LOCUS       DAFKMX010000318         2067 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.326, whole genome shotgun sequence.
ACCESSION   DAFKMX010000318 DAFKMX010000000
VERSION     DAFKMX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.326"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            136..1350
                     /gene="dpaL"
                     /locus_tag="LZB08_003683"
     CDS             136..1350
                     /gene="dpaL"
                     /locus_tag="LZB08_003683"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBM7317140.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            1477..>2067
                     /locus_tag="LZB08_003684"
     CDS             1477..>2067
                     /locus_tag="LZB08_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBM7317141.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          808 a          220 c          444 g          595 t
ORIGIN      
        1 gaaaaaataa taaaaacatt gcaatttcta tactaaagag tcatggaaaa atatctagta
       61 tatatttagt attggcatgt taattgctta tatattaaag tataaaaatt taacaataat
      121 ttggaggtat tttagatgtt aaaagaaatt aaatggaaag taaataattt gccaaagggc
      181 gataaggaaa attgtatcaa atttttaaat gaagaagaaa taactaaggt tagaaatttt
      241 cacaaaagct ttcctcaata taaagaaact ccattagcaa acctagaagg tttagcaaaa
      301 aaattaggtg ttgcaggagt atatgtaaaa gatgaatctt atagatttgg attaaatgca
      361 tttaaagtat taggtggttc ttattctatg ggtagatact tagctcaaag attagataca
      421 gatatatctg agttagggta tgacaaattg acttcgaaag aaataaaaga aaaattagga
      481 gaaataactt tctttacagc tacagatgga aatcacggta gaggagttgc ttggacagct
      541 aataaattag gtcaaaaatc tgttgtatta atgccaaaag gttcttctga atttagatta
      601 aataaaatta aaggagaagg tgcagatgct agtataacag acttaaatta tgatgatgca
      661 gtaagattag ctaatgatta tgcagaagct gatgaccatg gagtaatggt tcaagataca
      721 gcatgggacg gatatgaaga aataccagca tggataatgc aaggatatgg aactatggct
      781 caagaagcta tagagcaatt aaaagaatat ggagtagata gaccaacaca cgtatttgtt
      841 caagcaggag ttggttcact tgctggtgca gttcaagggt atgttgcttc aatatatgat
      901 gaatgcccaa taacagtagt agtagaggct gatgaagctg attgttacta taaatcagca
      961 gaagctggag atggaaaacc aagatttgtt ggaggagata tgccaactat aatggcaggg
     1021 cttgcttgtg gagagccaaa tactatagga tttgaagtat taaaaaatca tgcagcagca
     1081 tttgtttcag caccagactg ggtttctgct aaaggtatga gaacattagg taatccttta
     1141 aatggtgatg aaaaggtaat ttctggagaa tctggagcag ttacaactgg tcttttagta
     1201 gcagctatgg aaagagaaga tttagcagat ttaagaaaag atttaaaatt agatgaaaat
     1261 tcaagaatac ttttaataag cacagaagga gatactgacc ctgacaaata tagaagtata
     1321 gtttgggatg gagaatatcc aagtatataa ggaattacta aaatgcaatt attagatatt
     1381 ggtttacata tttagaagat atataagttc taaaatttag aaaatatata agtatataaa
     1441 atacatacat tataaaaaat ctagggggat aatgaaatgt taaacgaaca aagaaaacaa
     1501 gaagtaactg aaatatgcca aaaattaatt caaaatccta gttcttctgg aaatgaagaa
     1561 ggtgttgtaa aagcaataga agagtctttt gaaaaattag gttttgatag ttggtcaaga
     1621 gatagatatg gaaatatagt aggatgtata aaaggtaaca agccaggtaa gaaaatatta
     1681 tttgatggac atatagatac agttccagtt ccagatgcaa gtaaatggag tgttcctcct
     1741 tttgaaggaa aaatagttga tggaaagata tatggtagag gaacttctga catgaaaggt
     1801 caatatactg caatgatgtc tgctgtagcg tattttgcta aagacactaa tagagatttt
     1861 gctggagaac tatatgtagc aggtgttgtt catgaagaaa tatttgaagg tgtttcggct
     1921 agagagataa gtaaggctgt tcaacctgac tatgttgtaa taggtgagtc ttcagagtta
     1981 aacttaaaga ttggtcagag aggtagagga gaaatagtag tagaaacttt tggtaaacca
     2041 gctcactctg ctaatccaga aaaaggt
//
LOCUS       DAFKMX010000319         2043 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.327, whole genome shotgun sequence.
ACCESSION   DAFKMX010000319 DAFKMX010000000
VERSION     DAFKMX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.327"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(115..1029)
                     /locus_tag="LZB08_003685"
     CDS             complement(115..1029)
                     /locus_tag="LZB08_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBM7317142.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNNLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKMLV
                     KVIILDNKPVEVKIIGKAVIAFSTNIEI"
     gene            1285..1641
                     /locus_tag="LZB08_003686"
     CDS             1285..1641
                     /locus_tag="LZB08_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317143.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1756..1932)
                     /locus_tag="LZB08_003687"
     CDS             complement(1756..1932)
                     /locus_tag="LZB08_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317144.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(1985..>2043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          732 a          272 c          270 g          769 t
ORIGIN      
        1 aatgataaat atatattaac aaataataaa aaaattaatt tgattgtata taaattagtt
       61 tgttattaaa ttagtattaa aaagttcaaa aatttactta gaaggaatct ataattagat
      121 ttctatgttt gttgagaatg caattacagc ttttccaatt atttttactt caactggctt
      181 attatctaaa ataattactt ttactaacat cttgcctttg cgttttattg cttctccttg
      241 aactgcttca aatataaact ctgtgttggg tgtaggtatt actttataat gtacaaggta
      301 cgcacctaag ggtccattag catttcctgt aacagggtct tctgaaatac caattgcagg
      361 agcaaacatt cttccatgaa cttgtagagt ttctttatta tgtaatgtaa aaatataata
      421 tccattacat tttatttctt gacttatttt cgataattca tataaatttg gagataaact
      481 atttaatgtt gataattcct ttattccaat cataactttt gaatgtcctg tagatgatat
      541 ggcaatagga caatctgtac gaatatcttc ctttaaaata ccaaatgcat taagtaatct
      601 tttttgatag gtttgttgta atggtggaga tacttcaatt tttccttgtg tcataattat
      661 ttcataatca ttatcttttt ttataatatc aactggtaat attccagcac cagttttttg
      721 aaatactgtt ttgttattga attggttttc tactgctcta acataatggg cagcaatagt
      781 agcatgacca catataggaa cctcattagt tggtgtaaaa aatctgatat gtacatcata
      841 attttcttga tgatttttag ccaagttatt gaaaataaaa gcagtttctg aattatttaa
      901 ttctcttgct attttttgca tttgtgattc agataagcca tcagcatttg taattacacc
      961 agctggatta cctgtaaatt tttcctttgt aaatgagtca acttgataaa gacgaaattt
     1021 tttattcata ttatataatc ctttctaaac attagattag tttatctaca tagaaaactt
     1081 gagataaatt attatttatt attatctgtt taaataaaat taataaaata acatatgtct
     1141 atattaaaat ttaaacatat acatctaaaa aagtagtatt atgtaaggct ctatactaca
     1201 atcaagatta tattacgaat tagtacgcta aacaagaatg cactttttag ttagatactt
     1261 actattttga aagagaggat tgagatggat atagataata attttgaaga atttccaaat
     1321 attgaagaaa gacaaaaaga ttttaattgt tcaattggtt ttaccatgac tgtgatagga
     1381 agtaaatgga gggcaattat tttatggcat attttaaaac aagagccaat acgttacagt
     1441 caattaaaaa aagaagttgt tcatataagt cataagatac tgtcacaaga attaaagtat
     1501 cttgaaagag atggacttat taagaggatt tcttatccga ctattcctcc aaaagttgaa
     1561 tatttgtcta ctaaacgtgg taaatcatta gaatctatat tattagaatt atgtaattgg
     1621 ggtaaacagt atatgagata aactcaactg tttactttat gaaaaatata taatgaagct
     1681 tattttaatt agttctttaa tgaattataa aacgtagata aaactaaaaa gggttttcta
     1741 gaaaataaaa tttctttaat tatatctgct taccaatacc atcacttgat gcaaacaaga
     1801 ttctaccaca ttctctccat attctagtat aaaatcattt aattgtgtat ctataaaatt
     1861 gcagtaatcg cgtgaactgc atatatatgc agttagtata tgtgtacatc tatccatttt
     1921 atccataaac atatatcata tatcccccta aaaaacattc ataaaaacaa aaagaaaata
     1981 tctctttggc aactggctga cataactcat ttcataagtc ttagtcccta tagctttgcg
     2041 tca
//
LOCUS       DAFKMX010000320         1995 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.328, whole genome shotgun sequence.
ACCESSION   DAFKMX010000320 DAFKMX010000000
VERSION     DAFKMX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.328"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            230..1369
                     /locus_tag="LZB08_003688"
     CDS             230..1369
                     /locus_tag="LZB08_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBM7317145.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1395..1583
                     /locus_tag="LZB08_003689"
     CDS             1395..1583
                     /locus_tag="LZB08_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317146.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          847 a          163 c          282 g          703 t
ORIGIN      
        1 gttaagacat tgtcgagata ttataatata taactaatgt attataaata atagttgtaa
       61 acttcctgtg ccatatattt taaatgctta ttcttaaatt tttgcattat ttattaaaat
      121 ttattatgct ttaacgctaa attgagtttg ataattataa aacaaataat caaaaatagt
      181 gtaaaaatta atactttttg ttaatattaa tagataaaga taatctaagg tggtgattaa
      241 actgacaata gaagaataca aaaacaaata ctttactgga aataaaatcg tactttctat
      301 aaatagtttt gataataaag aactatacta tcatgaaact gaaaatatat taaatttaat
      361 aaatgaaata gataaattaa catcaagtaa gatatttaaa acaatagaag ataaaaatga
      421 tttggcacta gagtcttatt attctagtgt aatagaaggt gctttttcaa ctagaaagat
      481 tgcaaaatct ataattagag gaaagatgaa accaagtaat aaaagtgaat atatgattta
      541 caataatcat agagccttag aatatggatt agacaactta gataaattat atagtcacaa
      601 atttatatat gatttgcatc atatattggg cgaaaactgc ttggattcag aagaatatga
      661 gtatagaact gaaaaagtat atgtatgtga ttcaaaaggt gagattatac atacagggct
      721 tgaacctttg aagatatatg actttatgag taaattaata gattttatgg aaaatagcaa
      781 agtaagtaat ttaattaaaa gtgctataat tcatttttac tttgtatatg ttcatccttt
      841 tagtgatggt aatggaagaa catcaagagc cttatcatat ctatatttga ttgataaagg
      901 atatgatact ttcaaggatt tttcaatatc atatatgata tctaaaaaca gaactaagta
      961 ttataaggct atacttgatg tagaaaacaa aggaaataat ttaactgtat ttatagaatt
     1021 tatgctcaag tctataatac aatctattaa tgagatgcgt aatatgcatg atagaaaatc
     1081 tttagaaagt atattaaaag aagaattgtt tgaaaatgac ataactctat cagcaacaga
     1141 agaacatata ttaaaatata tttgcaataa agataactat tctatgacat tagaaaatta
     1201 tataaaaaag aataaaagta gatacttaaa ggctggaata aaggaaattg aattagttga
     1261 ccagttgatg gaagtgttta ataatcttga agagatagag attttgtcta aagaaaaaaa
     1321 tatatataaa gttaatgata agtacttaaa aatgcttgat atagactaat ttagataatc
     1381 tggagaggga atatatgaat aataagaaaa aggcattttt gatttctggg gctataaacg
     1441 taatttcatt aattattgtt ttttcattgt ttcattataa tattatagaa aatagtacac
     1501 aaaatatgat aataactgta atcttaattt ttatttttga aatgataaaa ataaaaatca
     1561 tagagaaata ctataaacta taaacaagaa atatcttttc taagagcata ttgtacaata
     1621 tgctcttaat ttagttatat agatttaata taaataatgt attttttgta gatgatttat
     1681 gtaagattta tgaccaattt gtgtatgatt tgtgttcttt aatcatgaca atattatatt
     1741 gtaaaaaata aatttaataa aaaatgactt ggaatttata ttctaggtct tttttatata
     1801 aaaatttaat aagtttcaaa tgttggtaac tttgacagta gaaaacgtct attgagataa
     1861 cgaagattct aattgagggg gagatatatt agcacttaaa gtagtatatg caacagatat
     1921 aatctttata aaagtaattt gaattaaaat gtaaatataa agaataatta aaaaaatatc
     1981 gcaattattt gtttt
//
LOCUS       DAFKMX010000321         1969 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFKMX010000321 DAFKMX010000000
VERSION     DAFKMX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.329"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            90..464
                     /locus_tag="LZB08_003690"
     CDS             90..464
                     /locus_tag="LZB08_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7317147.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            461..1324
                     /locus_tag="LZB08_003691"
     CDS             461..1324
                     /locus_tag="LZB08_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317148.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1324..1962
                     /locus_tag="LZB08_003692"
     CDS             1324..1962
                     /locus_tag="LZB08_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBM7317149.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          753 a          207 c          301 g          708 t
ORIGIN      
        1 aattagtttt ttctaaaatc agttgacata ttatatatat atttatataa tgtgtatata
       61 ctatatatac aatttaagga ggtgtcttat tgaatatagt tattagtaat tcgagtggga
      121 agccaatata tgaacagatt actacacaaa ttaaaagtat gattataagt ggagagttac
      181 cagaaggctc agctcttcca agtatgagac tattggcaaa ggaacttaga ataagtgtaa
      241 ttactacaaa acgagcatat agcgatttag aacgagacgg atttattgag acagttactg
      301 gtaaaggcag ttttgtatct agtaaaaaca tggattttgt caaagaagaa caatttcgtt
      361 taattgaaga atacctacaa aaagcagttg atacagcaaa aagtagtgat attacatatg
      421 acgaattaaa agatatttta tttttattat ataaaggaga atgataagta tggatgcaat
      481 aactataaaa aatttaaata aaacttataa agattttagc ctacaagata tatctttttc
      541 agtaccaaaa gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa aaacaactac
      601 attaaaagca attttaaatt taatatctta tgatagtgga aatatagaaa tctttggtct
      661 tgatagtaaa aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat ttgagggaag
      721 caactttcat gaaaatttaa acactgacca tgtttcaaaa atcatgagta aaatctataa
      781 aaattggaat gacaccttat ttaaagacta cttaaaaaaa ttgagagtac cagacaataa
      841 attaattaag gagttttcaa aggggaataa gatgaaactt agtattgctg tagccttatc
      901 tcataaacca aaattattaa tactagatga ggcaactagt tcacttgacc ctatcgttcg
      961 tgaagaaata ctagatatat ttttagattt tatacaagat gaagaacact ctattatctt
     1021 atcttcacat attacaagtg atttagataa gatagcagac tatattacat ttatacataa
     1081 aggaaaaatt gtatttagtg aaaataagga tgaattaatt gatactatgg gagttttaaa
     1141 atgtaaacct agtgattttg ataacctttc aagagaggat tattcatatt atcgtaaaag
     1201 ccaatttgga tatgaagtac ttcttaaaga taaacataga tttatctcaa gacatccaaa
     1261 ctgtattgtt gataatacgt ctatagaaga aattatgtta ttttatgtga gaggtgataa
     1321 ataatgaaag gtctaatttt aaaagattta cttaatttga aaggaaatat aaagtttata
     1381 cttttattca tcattatgtt tggctttatg tcttctttag gagatggaaa tgttaataac
     1441 tttataggtg ttattatagt tttatgtact acaatgattg tttcaacatt ttcatatgat
     1501 gatttgaata aatgggatag ttatgtactt acaatgccta ttaatcgcaa tgatattgta
     1561 ttaagtaaat atttaactat gttaatattt agctttatag gtgttcttgt ttcacttatt
     1621 gttagtgtaa ctattggtta ttttaaaaat acacttatat taaatgaaac actacttatt
     1681 aatgcactaa tcttatcaat ttcagtatgt tttggtagcc ttattctacc acttatttat
     1741 aaatttggta cagaaagagc tcgtttatta atgattttat gctttttagt tccaacacta
     1801 gctttattag tttttaaaag tattttagaa aatattagtt cacctatttc tatagaaatt
     1861 atacttaata cactagtata ttctcttcca tttgttgcta ttttactatt tgtgatttca
     1921 tattttatat catcaaaaat ctatagtaaa aaagaagtat aaaatatac
//
LOCUS       DAFKMX010000322         1935 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFKMX010000322 DAFKMX010000000
VERSION     DAFKMX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.330"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            128..244
                     /gene="rrf"
                     /locus_tag="LZB08_003693"
     rRNA            128..244
                     /gene="rrf"
                     /locus_tag="LZB08_003693"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            251..325
                     /locus_tag="LZB08_003694"
     tRNA            251..325
                     /locus_tag="LZB08_003694"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:283..285,aa:Asn,seq:gtt)
     gene            330..404
                     /locus_tag="LZB08_003695"
     tRNA            330..404
                     /locus_tag="LZB08_003695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:363..365,aa:Glu,seq:ttc)
     gene            410..485
                     /locus_tag="LZB08_003696"
     tRNA            410..485
                     /locus_tag="LZB08_003696"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Val,seq:tac)
     gene            491..567
                     /locus_tag="LZB08_003697"
     tRNA            491..567
                     /locus_tag="LZB08_003697"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Asp,seq:gtc)
     gene            578..652
                     /locus_tag="LZB08_003698"
     tRNA            578..652
                     /locus_tag="LZB08_003698"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:610..612,aa:Thr,seq:tgt)
     gene            667..751
                     /locus_tag="LZB08_003699"
     tRNA            667..751
                     /locus_tag="LZB08_003699"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:701..703,aa:Tyr,seq:gta)
     gene            761..834
                     /locus_tag="LZB08_003700"
     tRNA            761..834
                     /locus_tag="LZB08_003700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:793..795,aa:Gly,seq:tcc)
     gene            845..921
                     /locus_tag="LZB08_003701"
     tRNA            845..921
                     /locus_tag="LZB08_003701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:879..881,aa:Arg,seq:tct)
     gene            931..1006
                     /locus_tag="LZB08_003702"
     tRNA            931..1006
                     /locus_tag="LZB08_003702"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:964..966,aa:Gln,seq:ttg)
     gene            1018..1093
                     /locus_tag="LZB08_003703"
     tRNA            1018..1093
                     /locus_tag="LZB08_003703"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:Lys,seq:ttt)
     gene            1096..1184
                     /locus_tag="LZB08_003704"
     tRNA            1096..1184
                     /locus_tag="LZB08_003704"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1130..1132,aa:Ser,seq:tga)
     gene            1202..1292
                     /locus_tag="LZB08_003705"
     tRNA            1202..1292
                     /locus_tag="LZB08_003705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1236..1238,aa:Ser,seq:gct)
     gene            1301..1377
                     /locus_tag="LZB08_003706"
     tRNA            1301..1377
                     /locus_tag="LZB08_003706"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1335..1337,aa:Pro,seq:tgg)
     gene            1463..1538
                     /locus_tag="LZB08_003707"
     tRNA            1463..1538
                     /locus_tag="LZB08_003707"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1496..1498,aa:Trp,seq:cca)
     gene            1599..1675
                     /locus_tag="LZB08_003708"
     tRNA            1599..1675
                     /locus_tag="LZB08_003708"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1633..1635,aa:Pro,seq:tgg)
     gene            1682..1758
                     /locus_tag="LZB08_003709"
     tRNA            1682..1758
                     /locus_tag="LZB08_003709"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Ile,seq:gat)
     gene            1762..1837
                     /locus_tag="LZB08_003710"
     tRNA            1762..1837
                     /locus_tag="LZB08_003710"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1795..1797,aa:Phe,seq:gaa)
     gene            1845..1921
                     /locus_tag="LZB08_003711"
     tRNA            1845..1921
                     /locus_tag="LZB08_003711"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1879..1881,aa:Met,seq:cat)
BASE COUNT          487 a          443 c          525 g          480 t
ORIGIN      
        1 tttgatgttc attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaat
       61 aaaaaaagta ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag
      121 aaaaaaatgt ggttataata gcggagaaga tacacctgtt tccattccga acacagaagt
      181 taagttctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      241 acgtgaatgt tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg
      301 ggttcgagcc ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc
      361 ctttcacggc ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc
      421 tcagctggga gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc
      481 caccatttgc ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg
      541 agggttcgag ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag
      601 cagctgactt gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac
      661 taatacggag gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac
      721 ttcggtggtt cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta
      781 gagttctggc cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa
      841 gcgtgggtgc atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt
      901 cgaatccctg tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg
      961 actttgactc atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt
     1021 ctattagctc agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc
     1081 ggatagatca ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt
     1141 gacttcgaaa ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt
     1201 tggagaagta ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg
     1261 gtgcgagggt tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg
     1321 tagcgcacat ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact
     1381 taagctttta gttcagttgg ttagagtgcc caactcaggg ctagtaattc tagggtttga
     1441 gtccatgaag gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa
     1501 accgtgcgcc gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaaa
     1561 ttggatttta gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta
     1621 gcgcacatgg tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa
     1681 agggcctata gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga
     1741 gtccagctag gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat
     1801 ccccgtgtcg gtggttcgat tccgcctctg ggcaccagaa atacggcggt atagcttagt
     1861 tggctagagc gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc
     1921 atctaactat atcaa
//
LOCUS       DAFKMX010000323         1875 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFKMX010000323 DAFKMX010000000
VERSION     DAFKMX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.331"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            49..663
                     /locus_tag="LZB08_003712"
     CDS             49..663
                     /locus_tag="LZB08_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBM7317150.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDIAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            681..1043
                     /locus_tag="LZB08_003713"
     CDS             681..1043
                     /locus_tag="LZB08_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317151.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1149..1646
                     /locus_tag="LZB08_003714"
     CDS             1149..1646
                     /locus_tag="LZB08_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBM7317152.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          714 a          210 c          322 g          629 t
ORIGIN      
        1 aataaataca taaaatttaa actaatctat ttattggagg agataaaatt ggaagaagta
       61 attgcaaaaa ttattactat agaaaatgga ttcaaagaaa ttgaaaacat tgctaaaaaa
      121 attgtaaaaa ataatgactc aaaaaagtgt tattatctat caatctactt atataggtca
      181 gaatattatc aagtaagaga attggctgtg tttatacttg gttttatatc agtaactatt
      241 tctgaagctt tgttattttt aaaagataat gtgagtaaag atgaaaactg gagagtacaa
      301 gaaatactag ctaaatcatt tgattattat tgcagtgaag taggatatga aaaggcactt
      361 cctgttataa aagaatggct taattctgat agtccaaaca taaaaagggc tgtgacagaa
      421 ggtcttcgaa tttggactag tagagactat tttaaatcta atcctgacat tgctatatca
      481 ctactttcta gtttaaaaga agatgatagc gaatacttga gaaagtctgt aggtaatgct
      541 cttagagata taagtaaaaa atatccagat ttagtaaaaa aagaattaga ttcatgggat
      601 atatcaaata aaaaagttga gtttgtatat aagcttgctt caaaatgtat cttggcaaag
      661 taaaatggag gtgaacttat ttgaaacttc gtgatgagta tacttgccca ttagaactaa
      721 cacatgatat gataaaaggc aaatggaaat ctattatatt atggagatta cgcataggac
      781 caacttcctt atcaaaactt gaaaaggaaa tcaatggtat taatcaaaag atgttacttg
      841 aacaacttaa agaattaatt gaatttggtc ttgtagataa aaagacatat gaaggttacc
      901 cacttaaagt cgaatactca cttacaccta ttcgagggaa acaaatattg gatgctttgg
      961 ctataatgca agatatagga attagcttta tgttggataa tggtatggaa gatattctta
     1021 aggaaaaagg tgttttaaag taaatagata cccacaaaaa agtaagtaat ttaccattat
     1081 tttcaactgg tatataatta aattatagat atattctatt aatttattat aataggaggt
     1141 aaattattat gaaagtaact agtacaggta tagtaaatgg tgttatagaa gataaatacg
     1201 gtaaaagagg aactcagttc aatgaaggtg gtatgcctac atattcacta cctctaaagt
     1261 ttgaagatgc tccaaagaat acagtatcct ttgctgtatt tttagaagat aaagactctg
     1321 ttcctgtatg tgggtttgca tggatacatt ggttagctgt aaatataaca aaagatgagt
     1381 taaatgaaaa tgaaagtgta acagctactg attttgtaca agggactact agttggcatg
     1441 gcaaactagg aggtcttgac agattagagg ccagtgtata tggtggaatg gctcctccag
     1501 acagacctca tgtctatgaa atacatgtat ttgcacttga tactattctt gaccttgaaa
     1561 aaggattcta tatgaatgaa tttttcaaag ctattgaagg gcatgtttta gatacattta
     1621 cactaaaagg aagttattct aattaattag gttgactata gctaatcatt aattaaatgc
     1681 tacatttaat tgatgattag cttaattgtt attagaatat tttctgcact ttatcttaat
     1741 atatatttta gttgactggt attttaattt ctattagatt attatcaatt tatataaatt
     1801 gattttagct taaaacttac ttatctattt cagtttctat tagaattatt attagactca
     1861 ttaaaataaa agcat
//
LOCUS       DAFKMX010000324         1825 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFKMX010000324 DAFKMX010000000
VERSION     DAFKMX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.332"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..108
                     /gene="rrf"
                     /locus_tag="LZB08_003715"
     rRNA            <1..108
                     /gene="rrf"
                     /locus_tag="LZB08_003715"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            116..190
                     /locus_tag="LZB08_003716"
     tRNA            116..190
                     /locus_tag="LZB08_003716"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Asn,seq:gtt)
     gene            196..281
                     /locus_tag="LZB08_003717"
     tRNA            196..281
                     /locus_tag="LZB08_003717"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Leu,seq:taa)
     gene            297..372
                     /locus_tag="LZB08_003718"
     tRNA            297..372
                     /locus_tag="LZB08_003718"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:330..332,aa:Met,seq:cat)
     gene            380..454
                     /locus_tag="LZB08_003719"
     tRNA            380..454
                     /locus_tag="LZB08_003719"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            470..543
                     /locus_tag="LZB08_003720"
     tRNA            470..543
                     /locus_tag="LZB08_003720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:502..504,aa:Gly,seq:tcc)
     gene            549..624
                     /locus_tag="LZB08_003721"
     tRNA            549..624
                     /locus_tag="LZB08_003721"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Val,seq:tac)
     gene            630..706
                     /locus_tag="LZB08_003722"
     tRNA            630..706
                     /locus_tag="LZB08_003722"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Asp,seq:gtc)
     gene            717..791
                     /locus_tag="LZB08_003723"
     tRNA            717..791
                     /locus_tag="LZB08_003723"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:gcc)
     gene            801..874
                     /locus_tag="LZB08_003724"
     tRNA            801..874
                     /locus_tag="LZB08_003724"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
BASE COUNT          598 a          289 c          365 g          573 t
ORIGIN      
        1 aatagcagag aggatacacc tgttcccatt ccgaacacag aagttaagct ctctagcgct
       61 gatggtactt ggtgggaaac tgcctgggag agtaggacgt agccacgtta aatgttccaa
      121 ggtagctcaa cggtggagca accggctgtt aaccggtagg ctgtgggttc gagccccacc
      181 cttggagcca aaaaagccga agtggcggaa ctggcagacg cacaggactt aaaatcctgc
      241 gggacttacc tctcgtaccg gttcgattcc ggtcttcggc acttataaaa caatatcgcg
      301 gggtggagca gttggcagct cgtcgggctc ataacccgaa ggtcgtaggt tcaagtcctg
      361 cctccgcaac cattaaaacg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accaaattca attaaatatg cgggtgtagc
      481 tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc ttcacccgct
      541 ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag
      601 gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt agaatgccag
      661 cctgtcacgc tggaggtcga gggttcgaac cccttccagg tcgccaaatt aaataagcgg
      721 gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt cgaatctcgt
      781 ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact ataaattata
      901 gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg tgtcttttta
      961 tttataaact ttaaactatg taaaaattac agtatattct aatagagtta cagtatattc
     1021 attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt ttatgaaggc
     1081 aatcagttaa atataataaa tataactaat tagtaagagt tatatacatt atattgtata
     1141 ctttatggta tttatatatt gaaataactt atgaaattat ttataaagag attcatgagc
     1201 atatttttgt ctaaacaata agtataagaa attataaaaa attagagact atagtgaatt
     1261 tacaatttaa tgtaatattt ataagttata ctaaataaat tattatactg tgaaatggat
     1321 atataacttt aggtattgtg tattattttc actgtaggag attatatcta gttatacata
     1381 ataagtttta atgatagttt atagtaagat attaaaataa atttatctaa tctagatatg
     1441 aaataagtta ttactttaat ataaaaatga atctaatgga ataatattga tgataagtaa
     1501 tatttagatt ttgtatttgt ttatagttaa taagtggatt atagaattta taataattaa
     1561 tatatattaa acaaagatat attaagatat tttagaacat aaaactgaat taaggcgcga
     1621 gtactatttt gtattcgcgc cttgatggtt tgagttattt gagtaaaaac taactttaat
     1681 aagaaatatg tatttattaa gttgatattt ttaatgtgtt tagagattat ttaacaatct
     1741 ccaaatataa aactagaatt atttacataa aagttaaata gtttgtatag agttagattt
     1801 atatagtata atacttagtg taaat
//
LOCUS       DAFKMX010000325         1823 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFKMX010000325 DAFKMX010000000
VERSION     DAFKMX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.333"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            94..663
                     /gene="mocA"
                     /locus_tag="LZB08_003725"
     CDS             94..663
                     /gene="mocA"
                     /locus_tag="LZB08_003725"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBM7317153.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            660..1679
                     /locus_tag="LZB08_003726"
     CDS             660..1679
                     /locus_tag="LZB08_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBM7317154.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          731 a          164 c          348 g          580 t
ORIGIN      
        1 tttatatgat attatatata tagataatat caatagagat attataaaaa tcatagaact
       61 atcaatatat tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca
      121 ggctttgcca gaagaatggg agcaaataaa cttttattaa attataaagg ctgttctata
      181 atagaacatg tatttagaga agttagcaag attgattttc atcaagttgt agtagtaagt
      241 caatatgaag aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa
      301 aatgcaaata taggtcaaag tgaatctata aaattaggag ttttaaatag ctcaaaatgt
      361 gatggttata tgttctttgt aggagaccag ccatttattg ataacttata tatagataaa
      421 attataaata cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg
      481 tgtggaaatc cagtcatatt cccatatagt aagaaagaag aattgttggc acttaaagaa
      541 gatgaaaaag gtaaaaccat tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct
      601 aaagaaatgt tatttgatat cgatacaaaa atggactatg agagaattgg gggtaaatta
      661 tgaagctaat aaaaactata gatgctgttg gacaagtttt atgtcacgac ataactcaaa
      721 taatacctgg agaatttaaa ggaagaaaat ttaaaaaggg ccacatagta aaagaagaag
      781 atattccagt gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag
      841 gaatggttca tgaaaatgaa ggagcattgt ttttaaaaga actaactgct ggagaaaatt
      901 tagattttag tgaaataaaa gaagggaaaa tagattttat agcagcatgt gatggtctat
      961 taaagataga tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta
     1021 ctcttcataa taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac
     1081 ctttagttat agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa
     1141 tagtaaatgt agtacctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag
     1201 tgttttatag cagaattgtt gataaatttg gaccagtaat agaagaaaaa gtaaaaggct
     1261 ttggttgtga agttatagga cagactatat gcccagatga taaagagatt ataaagagtg
     1321 caataaaaga atttataagt caaggagcag aacttatctg ttgtactggt gggatgagtg
     1381 tagacccaga tgatgttacg ccaacagcta taaaagaaac tggagctgat ttagtgacat
     1441 atggttcacc aatattacca ggagctatgt ttttacttgc ttattacgga gaagttccaa
     1501 ttatgggtat accaggatgc gcaatgtatc acaaaactac agtatttgat atagttttat
     1561 ctagggtttt aattgatgaa aagcttgata agtatgatat agctagatat ggtcatggtg
     1621 gcctttgtat gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa
     1681 gttattaaaa aagagtattt acaaattaag tactcttttt aaatatgaaa caaaacagct
     1741 agaatgatgt tttaaaaaaa gcatatttat ttagaaatta taataactta ttttcaatgt
     1801 gtaaaagaat gaataaagtt gca
//
LOCUS       DAFKMX010000326         1788 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFKMX010000326 DAFKMX010000000
VERSION     DAFKMX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.334"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            151..1497
                     /locus_tag="LZB08_003727"
     CDS             151..1497
                     /locus_tag="LZB08_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBM7317155.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNTVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT          572 a          229 c          294 g          693 t
ORIGIN      
        1 ttgatttatt aaattcaaat tataattata ttctttacat tttaaaaaaa tatatgatat
       61 tttgatacaa tttatttata gtttggatat aaagtttatg ttaagattat actataataa
      121 attaataatt tttttatttg gaggaataag gtgaaagatt taactacagg tcatgaggga
      181 aagtccatat tcttttttgc catgcctatg cttattggta gtttatttca acaactatac
      241 aatacagctg acagtataat agttggtaga tttatcggaa aagaagcaat ggcggcagtc
      301 tctggagcta accctataat gtttttattg gtggcagcgc ttatgggtgt tagccttgga
      361 ttttctattt tagtgtcaca attttatggc tcaggagatt taaaaaaggt aaaagctact
      421 attgatacta catatatact tttatttatt ggttctatat taataagtat tcttggaata
      481 gtatttggtg gccccatgct taagcttatg aatacaccag aatccgtttt tgctcaatca
      541 aaattgtatc ttactataat atttagtgga atattatttt ctgctggata taattcagta
      601 agtgcaatac ttagaggact tggagattca gtaactccac tttattttct gataatagcc
      661 acaattctaa atacagtttt agacttaact tttattgtag tgctaagaat gggtgttgag
      721 ggcgttgcac ttgccactat aatggctcag gcagtatcat ttataataag tataatttat
      781 ttaaataaaa aacatgaagt gcttaaattt aaaatcaaag gaattgtata tgataacaaa
      841 atatttaaag atggacttag attaggactt ccaagtggag ttcaacaaat gttgttttca
      901 attggaaata tggctcttca atttttagtt aatagttatg gtacatctgc tatggcagct
      961 tttggtgctg gtcttaggat agaaaatttt ataagcttac ctattatgaa tcttggttct
     1021 gctgtttcta cttttgttgc tcaaaacata ggtgctggag aaaatgaaag agttaaaaaa
     1081 ggcattcgag aatctataaa aatgacctta gttttagcag ttacagttat tgctttaatt
     1141 ttgctattta gggaaaatct aattgctcta ttcaatactg ataaagatgt cattaaaatt
     1201 ggctcttctt acttatttat aataggacca ttcttcttat ttattggaac ttcttttgta
     1261 ctttcaagtg caatgaaagg tgctggtgat tccatgtttg cacttataag ctcaatagta
     1321 tctttatggc ttggaagact tccagcttct tatatgcttt ctaaattttt tggaacagac
     1381 ggaatttgga tgggtattcc ttttggttgg actttaggtc ttattgttac agttatctac
     1441 tataagaaag gacattggaa aactaaagct attgtaaatc atagaatcaa tgaataatct
     1501 ttttataaaa tgtattcaca ttcattgtat tcacctccat tatgatataa taaattatgg
     1561 aggtgaatat aaaaatgggt attattctta agccaaaaca attttatatg actgttatag
     1621 ctaccataat tctctacttc tctcttaatt acagtttcaa cataaaattt tctatatgct
     1681 tatctttttt aactatcttg atatgggctg tagactcagt tgaaaaaact tttgttgcat
     1741 tgttttttgt aattctagcg tttatattta acattgcacc aataaagg
//
LOCUS       DAFKMX010000327         1781 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFKMX010000327 DAFKMX010000000
VERSION     DAFKMX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.335"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1052
                     /locus_tag="LZB08_003728"
     CDS             <1..1052
                     /locus_tag="LZB08_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBM7317156.1"
                     /translation="LIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIA
                     GFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLL
                     NTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEA
                     KSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVI
                     DALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNR
                     YLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYF
                     QINVLLVISLHCASI"
     gene            1091..1576
                     /locus_tag="LZB08_003729"
     CDS             1091..1576
                     /locus_tag="LZB08_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317157.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
BASE COUNT          740 a          186 c          261 g          594 t
ORIGIN      
        1 atttaattga acaattagta tatggattta cagctccatt aaattacttg atatttacta
       61 gtgatataaa tacagatatt tataaatttg atataaatat gggactatct tctttaatta
      121 ctattataat agctggattt atatattggt atagttctaa aaagtttaat attaaggtca
      181 tgaactttga taaatatata gcaattttga tgattccatt gttgcttata atattattta
      241 tgcaatcatt tgaatatagt tcaaatataa atatagatac aagtcttggg attgtaaagc
      301 tgctattaaa tacttctata actgaagaaa ttcaagcttt tcttttttct ataattggaa
      361 ctatatgtgt atttttttct ctatttacct ttaagaaact aattcaagct ctcgaagatg
      421 acaaggagag ggcaattatg aatcaacaga ttcatgctca aaaaaactat attgaagaag
      481 cgaaatcacg attatcacaa actatatcct ttagacatga ttttaataat catttagcta
      541 ttgtcaatgg attgttgaaa aaagaccaaa ttttaaaagc acaagattat ttaaataagc
      601 ttgaaaaagt aacaagtgac ctatcatttt cttgtaatac acagaatatt gttattgatg
      661 ctcttttcaa caataaatta agtatagcaa aacaattagg catagaggta gattgtgaag
      721 taattatacc ccaaagcact aacatgaacg attttgacct atgtatagtg tttgctaatg
      781 ctattgacaa tgcaattaaa gcttgtagag ttgtagataa acaaaatagg tatttaaagc
      841 tttccactaa gttagagggt ggatttttta tgatagaaat aaaaaatagc tataatccaa
      901 taaataatta tacaagtggc tcaggtatag gtcttttaaa tataaaagaa attgctaaaa
      961 aatatcaagg ggctataagt atagataaga catcagacta ttttcaaata aatgtattgc
     1021 tagttatttc attacattgc gctagtattt gagaacaatt tcattgaaaa tggtatttgt
     1081 aagctatatt atgtacttga atagagaaag tttgatatat agaaatgagg aggttactaa
     1141 aatgaaagtt ggaaatataa atggtcaagc accaattaca aatggtttag atagctgtca
     1201 aagtgaggta caaggcttag aacaaaagaa aaaatcatta caggatgaaa tagagaaagt
     1261 aagaagtgat aaaagtattg atggtgaaac agccaaatta aaaatagaag ctttaaaaaa
     1321 agaaatggaa gagattgaaa ataaaattca agagttaaaa ggaaataaag caaaaaaaac
     1381 aaataaagaa gaaaataatg tttcagaagt aaataataat ctattagaga gaaaaaaaga
     1441 accttctaat aatgaaaatg taaaacaatt atatgatgtt tttgaaagaa gcaatgtaga
     1501 cgaacaatgg gataatactt atcgtgttct ccaagatgat aaaaacttaa aagtaaaatt
     1561 taatagacct atatagttta tttgtaaaaa tagttaatat tttaattatc accacttttt
     1621 agaagaatac acactttatt ttaaagaccc acatatttat ttacagttag cacatttata
     1681 tgtggtttta taactattat ttacaagtat atattgataa cttttacata gtatataatg
     1741 tttaaatact tattcaaatt tatctataca taaacaaaaa a
//
LOCUS       DAFKMX010000328         1724 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFKMX010000328 DAFKMX010000000
VERSION     DAFKMX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.336"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(394..1101)
                     /locus_tag="LZB08_003730"
     CDS             complement(394..1101)
                     /locus_tag="LZB08_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBM7317158.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          560 a          290 c          152 g          722 t
ORIGIN      
        1 attttgaaat ttgatttaaa gcataataaa atatataaaa aaatatgtaa aatggtacta
       61 taaaattttt aatcttcatc aatacctttt ttgctttcat tgttaaaact attttttcta
      121 ttagtttatt tcttataaaa caaacattta gtattaaaat atatatcaca caatagtatt
      181 tgcaatttaa ctttaaaaaa ataaaaatac ccaactgacc taagcaactc gattgggtat
      241 aaataatcta aaaaatcatt tttgtataaa ctctttattt aacttacatt tttatttacc
      301 ttctacttta attcaactta tatttctatg cttcttatac tctcattcaa cttatatttc
      361 tacttatctt atacttttat aaattcatca caattattta gttcttgcta tcaaagaaat
      421 tgtattaaaa tactttccat tctctacttc catcattttt ttgtcattta tagcatatga
      481 attatcacaa tttatccaat ccttccaagc ttcatcatga caatttaaag cttcacattt
      541 ctctacaatc acagactccg attcagacca aagtttattc caccatgtaa ttgaatggaa
      601 gttcatatta tcttgccaaa atggaatgag ttcatctggt atacaatcca cgaaatcttc
      661 ttttaatcca ggcattgcca tagcaagtat tcccccctct tttacaagag gtgatatatg
      721 attgtctaaa aatccttctt tgtttccaaa atagtgataa gaatcgatac ttatcaccgc
      781 atcaaagaaa ccttctgcat aaggtagttc atgtgcctct gcctgtattg gaaatatttt
      841 atcatccaat ttaaactctt taaatctttt atagttttca gttggttcta tccataaatc
      901 tgttgcaaat acagtagcat caaattcctt tgctaaaaat atcgaagata tacctttacc
      961 acaacctaag tctaaaatac gcattccctt ttctagtttt atcttacttg ttaattcttc
     1021 caatattttt atacaattag gtcccatcat atttttcatg agaaagtcct tatcgtattt
     1081 attactcttt gtaaatatca ttctatcttt cctttcccta tcagttatta tttattaaat
     1141 tttatacgtt tgatttttta ttttttatat gcttcttata ttgagcaatc tcttttttat
     1201 tcattcttta acagacaatt ttaatgtgta attttatcca ctctgtgata gtaactttaa
     1261 tgaataatct tatctactgt gatagttaac tttgatgggt aattttatct actctatgat
     1321 aattatttta gtgagtaatt ttatctactc cacagcaatc gctttaatga gtaatcttat
     1381 ccactctatg ataattattt tagtgagtaa ttttatctac tctacagtaa tcgctttaat
     1441 gagtaatctt atccactcta tgatagttat tttagtgagt aattttatct actccacagt
     1501 aatcgcttta acgagtaatc ttatccaatc tataatagtc actttaatga gtaatcataa
     1561 tttttatatc attttagata cttactctaa taagaagtca ttttctaata cttaataagt
     1621 tttaattact tccactttaa cctatatact aattaactta tttaatttag tgcatatttg
     1681 acactttacc ttttattata ccatttatta tcataaatat aata
//
LOCUS       DAFKMX010000329         1717 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.337, whole genome shotgun sequence.
ACCESSION   DAFKMX010000329 DAFKMX010000000
VERSION     DAFKMX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.337"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..876)
                     /locus_tag="LZB08_003731"
     CDS             complement(<1..876)
                     /locus_tag="LZB08_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317159.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLY"
     gene            complement(881..1618)
                     /gene="cas6"
                     /locus_tag="LZB08_003732"
     CDS             complement(881..1618)
                     /gene="cas6"
                     /locus_tag="LZB08_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBM7317160.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGKERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
BASE COUNT          549 a          277 c          132 g          759 t
ORIGIN      
        1 atatagtcta attgaactta ttttttcatg caaaagtttt ttataaaatg atttatctga
       61 atacattttt cttttaagca ttatcttatg atttaaaaat gctaattctc ttaaatcatt
      121 cgtatatgca aaaaccattg gtctttttaa atttatatat aaaagacaca ttaagttgaa
      181 aaattcacac tcaaaacaaa tattactttc aacttgtcca cttcttttaa agcctgattt
      241 ttctgcacct ctaaataaaa agttatactt agagtccttt cgttctctta aatcaatatc
      301 tagttttgtt gttttattta tattgcaaac attacataca ctatcttttt cttcatattg
      361 tgtcaaatca agtttatcta tttcatctat ctctatattt gatttaatta cagtatctac
      421 tattcttttt acatttgttt ccatattttc taaactcgtg tttaatccat acttaccact
      481 atttctaaca tatggaaacc tcctgttttt aaaatctttt ttaatatctt ccttttgttt
      541 agcagttgca gaagatttat ccaattcttc aataaaattt acatacgtta aattatctat
      601 attactaaat attttttcta aggtttcctt tttcccattt ttaaatacct taaaaagttg
      661 attttctaat atataatcag atatatactt agggtctata tattctgcag attctaaaga
      721 taaataattt ctatttaact cacaagtaaa ttttagctta taatcatttt tttctagttc
      781 gttttttaaa tccattagta attcataaaa gtaaacaatg ccaatatctc ttacaatatc
      841 acctgatcta taaaatttag cttttatttc tctcacctcg ctactttaat atatcaacca
      901 ttcctgcatt ttccgtcctt ctatatccaa ttcccatttt atacagtgca tttaaatctt
      961 ccatatctcc ttttaaaaaa aatgttcctg cataagcatt tatatagtaa aattctctca
     1021 ctttaaatcc acgaatcttt tcttttaaca ctcttttttt gaagttaaga ggtataaatt
     1081 ccaatggctt tctaagccca ctacctctta tactctccag agttagatta gctatatagt
     1141 ttaaacattc tatataattt gaatcttcta catctaaata tttaccttct ttattcttta
     1201 ctattatagg agacatagtt ttaaatataa caccattttc ttttatctcg tgttctttat
     1261 gtatttttat attttttatt ttaaatctat aacctttata ttctagctcc tttactttta
     1321 aaattccatt gtataattta ctgaacatag aataattata aaaacttata ttgaatatta
     1381 tatctccatc aacaagtaac tcatttttgc ttcttataag atttggtatg taaatggctg
     1441 tatttatatc ctttatttgc ttattgtttt ttctttcttt cccatagtta aatatgtttt
     1501 tagcaaaatc tttgttttct gcatttaaaa tattttttat aaaggaaact gacaacattg
     1561 aattttcatt tggaaatttg ccattacatg attccataaa aattgaaatt ctgctcatat
     1621 tttcacctcc tactttctag tataattcat tattctctat ttgtaatata aaaataaaac
     1681 atatcataaa atgatttttt gataataaat ttaattt
//
LOCUS       DAFKMX010000330         1715 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFKMX010000330 DAFKMX010000000
VERSION     DAFKMX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.338"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(188..1516)
                     /locus_tag="LZB08_003733"
     CDS             complement(188..1516)
                     /locus_tag="LZB08_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBM7317161.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          619 a          321 c          197 g          578 t
ORIGIN      
        1 gtttaatttt ataatcaaaa aactaaaaat aaaatgaata ccttaaaata aactttttag
       61 ttcatggaac gctctttttt tgtaaaatta aacatatctt tatcatttta acaataaaaa
      121 agagacttat ctctatttga gacagtctct tttattttaa ttatgaagca tttgtaaact
      181 acttggttta tctttttact cctctagtca tataagcata cactggtaag cctatagctg
      241 ttattacaag acctcctata gatatcatag tagttttcat acctgcaaaa cacaattgat
      301 taactacaac aaatatacca cttgctatag ctattatagg aacaatagga tataatggaa
      361 ctttatactc tcttggtata tttggatgag tctttctaag tttcataacc cctataaaag
      421 ttaatacata gaatatccaa gtagcaaata cagctaagtc agttaataaa ttaaattgac
      481 ctgacaaagc atatatagat gcaataacag atactaatgc tattgcattt gctggaactt
      541 gagcagaatt taatttagaa aatataccat actttggtaa agtcttatct gtagctaaag
      601 tatatgccac ccttgaacca gttagtaaaa atccatttaa tgctccaaac actgaaatta
      661 atatacctac agatattatt tttccaccca ttgaaccaaa tataacttca gcaactgctg
      721 atgcaggaga agcataattt gctaattcat tagctggtaa tacccaaaga tacgctaaat
      781 ttattataaa ataaacagcc ataacaactg acaatccacc tattatagct tttggaaggt
      841 cttttccagg attcttcatt tctccagcta aagtaccaac attcatccat ccatcaaaag
      901 caaataatat agcaattaaa acttgaccta gtacactacc caggcttaat ccttcaccaa
      961 ccataggtgt aagtattgga ttatttccac cacctttaat aaatccaaca accattatta
     1021 aaataagtgg tattaactta caaattgtag aaacagtttg tatgactcct ccagtttttg
     1081 aacctaacat attaagccct gcaactatta ctataattcc aattgctatt ggaagtaaaa
     1141 gtgatggact accaattagt gctgatgctt gttctccaaa tataacacct aatgctgcca
     1201 tcatccctgg ataaaataat acaatttgca tccatccagt taaaaaacct aatttttctc
     1261 cataaatttc ttttatatat accatcattc caccagtttt tggtatcgca actgatacct
     1321 ctgcagctgt aagaccagct gttatagtta tgattccagc tattaaccat gccaatatac
     1381 caagacctgg agctcctcca gttaatgtat agactgcttg tggtttaaaa aatacgcccg
     1441 ctcctataac ggaaccaaca acagttgata atgctgctga tacgccaaga gtctttttca
     1501 gttcattact tcccattttt ttcctcctca ttaatcctat ttaattttaa tttcatagtt
     1561 aaataagcat atattatgcc aatatataga aaaatagctt tattatttat taaaataaaa
     1621 taaactctac ttagctttag taatctttta tttttagtta tgtttaaata taaaaaaatt
     1681 ataaaaacca aaaatccaaa tataataata cttct
//
LOCUS       DAFKMX010000331         1678 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFKMX010000331 DAFKMX010000000
VERSION     DAFKMX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.339"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            167..901
                     /locus_tag="LZB08_003734"
     CDS             167..901
                     /locus_tag="LZB08_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBM7317162.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGDFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1044..1535)
                     /locus_tag="LZB08_003735"
     CDS             complement(1044..1535)
                     /locus_tag="LZB08_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBM7317163.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMNKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          628 a          189 c          242 g          619 t
ORIGIN      
        1 ttatatataa ggaaaaattt tttaataata taaatcatat tcatttatat tattaaaaaa
       61 tttgtttata gaaactttta ttattttata taaaagtgat aaaaacttat attttgtatt
      121 tagtagagtg ttgctaagtg aatatattta aaatggagga aactttatga aacaaaataa
      181 atatgatgat gatagatttt ttaataaata cagtaaaatg gaacgttcta caaatggatt
      241 agcaggtgct ggagaatggc atgttttaaa aaaaatgtta cctgatttca aaggaaaaag
      301 agttttagat ttaggatgtg gatttggatg gcattgtcaa tatgcagttg agaatggagc
      361 aatttctgct gttggagttg atatttcaga aaaaatgtta aaagaagcta gaaacaaaac
      421 gcaacttgat aatataaatt acatatgtat gcctattgaa gatattaatt ttccaaaaga
      481 tagttttgat gtagttatta gttccttggc atttcattat attcaatcat ttgaagatat
      541 atgtaagaat ataagtaatt gtctttcaaa taaaggtgat tttgcatttt ctgttgaaca
      601 tcctatattt actgcatatg gaagtcaaga gtggtactgt gataaagatg gcaataatct
      661 acactggcca gtagatagat attttgaaga aggagtacgt aggtcaaatt ttctaggaga
      721 agaggttata aaatatcata aaacattaac aacttattta aatacgttaa tgaaaacagg
      781 atttgaaata ttagaaatta ttgaacctca accagaagaa aaactgttag atacaattcc
      841 tgatatgaaa gatgagctac gtagacctat gatgttactt gtatctgcaa aaaaaagata
      901 agtaaatata aataatcatt attgtagaaa aaaagttgta gaaaataatt atactaatgg
      961 agatgattat accagtggta ttaattacat cagtagtaag gaggcaaaag cattcgttgc
     1021 ttttgccatt ttttaaattg caattatact tctacgtagt atatttcgtt tccattttct
     1081 tctataagct ttttcatatc ttcaaatttt aaaaatacag tagcagtatt atcatttgga
     1141 tgtataccta ttttattcat ttttaataaa tccttatcaa atacaacttt tacaacagcc
     1201 tctttatcat ttaatatacc aaaaggagtc acagctcctt tagtaagttt caagtatttt
     1261 tgaagtcttt cttctgatgc aaaactaaga ggtttactat caatttgttg acgaatatct
     1321 tttaaatctg cttttttgtc tttacctaat acaactaaaa aatgttcttt tcctttagca
     1381 tcacgaagaa ataggttttt tacaacaaat tccatatgta atgtttttaa ttcttccatt
     1441 tcttcaattg tataaactgg tggatgattt acagcctcat aaggtatgtt taactcatct
     1501 aattttttat atacttcctg ttgttgattg ttcatgattt cctcccatta gaaatttatt
     1561 ttatatataa ataatattat cacagaaaac tttaaaagaa aacgttttat ataatataga
     1621 caatagatta tatttttata aagaagcaag aatactcaat aaaacttaaa gaaattaa
//
LOCUS       DAFKMX010000332         1650 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFKMX010000332 DAFKMX010000000
VERSION     DAFKMX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.340"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            256..957
                     /locus_tag="LZB08_003736"
                     /pseudo
     CDS             256..957
                     /locus_tag="LZB08_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          650 a          172 c          211 g          617 t
ORIGIN      
        1 ttttatatgt ttttagatta atataaaata tcacaatgaa atgttgtaca aagtaatata
       61 taataattat atgaactata aaattgacta aacttattga ctaataaatt agatgaaaag
      121 ttgagactta ctgaaaataa atacaatgaa atatatacta caagtattaa attaaatctg
      181 aactttacat actattagaa taagtattat aagattataa tgatttaaaa atattaaaat
      241 aattgaggta ataacatgaa caattccttt ttagataaat tgattgagac aaaagaactt
      301 aaaaattcac tttataatgt gttaaaacac aattttcttt atcattctaa taagatagag
      361 ggcagtactt ttactactga agcattagca cttttactcg ataagaatgt tgttacagga
      421 agacatactt tagatgatgt acaagaaact gttaattcta gttatgtatt tgatacaata
      481 attgactcac tgaaagagaa aataacacat aatttcttaa ggaatctaca ttcaagttta
      541 atcttcaata caaccctaca ttccaggtag atgtctggta tatataaaac tatacccaat
      601 atgatactag gaactgatgt aagcatagca caaccatttg aagttgagcc aaagcttgat
      661 gaattaattg aatggtacta tagtcagagt gaagtatcta ttaaagttat agctgaattt
      721 cattatagat ttgaattgat acatccattt caagatggta atggtcgcat aggtagattt
      781 gttatgctaa aacagatgtt agaaaataat cttcctataa aaattgtatc ttgggatagt
      841 gaggaattgt atagaaactc cttaaattct tgtagtttag gtaattatgt ccctttaatc
      901 gaatatttat caagtttaga agattttaga gaagtatata aaatgttatg gaaataattg
      961 ttttcagcaa aaaaagtttt tatataaata tatttttatc tatgctattt aacgctgatt
     1021 tcttagttat tcttagcttg gactatatcc actattttaa ctatataaaa tagtggatat
     1081 agtttaacta tctctttata actattttta tgtatcttag ttgtttctta atatggggac
     1141 agttcataaa actaaaagtg atttttaatt ttatcatttg taatttacca ttacatcaga
     1201 aaaatgctta tcattagaat tttcataaaa ggtacctatt tttttcaatt aactaaatca
     1261 attctattag tcttaatctt tcgtatttca aattgataat ataagaaaat aaaaaataaa
     1321 aaattttaaa ttttttattt attgcgaaaa aaataaaaag gtgctatact ttttgtaaca
     1381 tattattcaa attattaaga gatagtaaac atttatagtt taatttgtag tacactagca
     1441 tgatttgcag aacaatgaat taatctatag tataatagtt taatttaaag tacaacgatt
     1501 taatctatag tacaataact tgatttgaag tacaacgatt taatctatag tacaataact
     1561 tgatttgcaa tacaatgaat taatctatgg tataatagtt taatttgaag tacaatgaat
     1621 taatctatag tataatagtt taatttgaag
//
LOCUS       DAFKMX010000333         1642 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFKMX010000333 DAFKMX010000000
VERSION     DAFKMX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.341"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            26..1624
                     /locus_tag="LZB08_003737"
     CDS             26..1624
                     /locus_tag="LZB08_003737"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBM7317164.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          634 a          200 c          265 g          543 t
ORIGIN      
        1 ttcaagtttt gaaaggagta ttaaaatgag tttattagtt ttcggacata aaaatccaga
       61 tacagattca atatgttctg caatttcatt agcacattta aagaacaaac taggaataaa
      121 agcaacagct tatgctttag gagatatcag aaaagaagct aaatatgcct tggattactt
      181 taaggtagat gcacctgaaa ttttagataa tgttagaata caagttagag atttaaatta
      241 tgataaagta gtacctttaa caccaacttc atctatatta gaggcttata accttatgga
      301 tgaaaaaaat gttaaaacac tacctgtaac ttatgatgat ggaacttttg ctggtatcat
      361 tactatgaaa gaaatagcta aaaatctact tcatcaacac tttacaacta tacacacatc
      421 actatcaaac atttgtaaaa accttaatgg tactattctt gtggactgtg gtgaagatat
      481 aaaaggtaga gtagtcactt tatcatttgg aatggagact ctaatagaaa ctttaaaaga
      541 aggagacata gctattgttg gagatagata tgatagtatt gaatatgcta ttgatacaaa
      601 agttaaactt ttaatactta ctaatcatac agagatttca aaagatttgc tagctcttgc
      661 taagataaat ggagtgtctg ttgtttctgt tgaaagtgac aattataaaa cttctaatgt
      721 aataaatcaa tgtagtttct tagactctat agaagctaca gaaaatttaa ttacattcaa
      781 agaagatgat tatatggatg aaataaaaga gattatgcta gaaacaaact tcagatctta
      841 tccaatatta gatgatgata ataagttttt aggtttagtt tcaaaaggtc atctattaaa
      901 cccatctaaa aagaatgttg ttttagttga ccataacgaa tatgctcaaa gtgctgatgg
      961 aattgaacaa gcaaacatag ttgaaataat tgaccatcat aaacttggtg gtatttctac
     1021 tgatgttcct atgtcattta gagttatgcc tgttggatgc aatagtacta taatttatca
     1081 aatgtacaaa gaaaataatg ttgagatacc ttatgaaata gctgggcttc ttttatctgc
     1141 tatattatca gatacattat tatttaagtc tccaacaaca actgatatag ataagaaagc
     1201 ttgtgaagaa ttaagtaaaa tagcaaaagt agacatggaa aaatacgcta tggatatgtt
     1261 taaatgcggt acttctttag atgaatatac tatagaagaa atagtaaata tggactttaa
     1321 agaatttaat atgagtggac atagagttgg tattggacaa gtatttacct tagacataga
     1381 ctctatattt gctaaaaaag atgagttttt atcttatata aattcgactg attatgataa
     1441 attaattctt tcagttacag atataatcaa agaaggttct tatttaatct ataaagcaga
     1501 agatagctta attgctaacg catttggtgt tgaaggtgtt caaggtacat ttgcaccagg
     1561 tgttgtatca agaaaaaaac aattagtccc taatttaact actgcaatca aaaattttaa
     1621 ataatataaa atttttaaat aa
//
LOCUS       DAFKMX010000334         1620 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.342, whole genome shotgun sequence.
ACCESSION   DAFKMX010000334 DAFKMX010000000
VERSION     DAFKMX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.342"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(622..1134)
                     /locus_tag="LZB08_003738"
     CDS             complement(622..1134)
                     /locus_tag="LZB08_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317165.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          498 a          258 c          110 g          754 t
ORIGIN      
        1 tttaatttat tttatatttt tgacgcaatc gttttttatt caatcatttt atattcttga
       61 tacaatcatt cttacactta attatttttt atttttgaca caatcgtttt ttgttgaatc
      121 attttatatt cttgatacaa tcattcttac acttaattat tttttgtttt tgacacaatc
      181 gttttttatt cagtcattta atattttcaa cacaatcatt cttacattct attattgttt
      241 gtttttgata caactgtttt tattcagtca tttaatattc ttgacacaat tattcttaca
      301 ttctattatt ttttattttt gacgcaatcg ttttttattc agtcatttta tattttcgac
      361 acaatcattc ttacattcta ttattgtttg ttttgataca actgttttta ttcagtcatt
      421 ttatattttc gacacaatta ttcttacatt ctattatttt ttatttttga cgcaatcgtt
      481 ttttatttag tcattttata ttttcgacac aatcattctt acattctatt attgtttgtt
      541 tttgatacaa ctgtttttat tcagtcattt aatattctca acacaattac cttttcattc
      601 aatcaaagta atctttaagc attatttttc aagctcatca aagtaattat atacgataac
      661 ctcacctgtc tctttatctg tagtctttat aatgccctta gctccattta cctcttcttt
      721 tgtaaattca tcatctaaca caactttgaa tttatcttcc tttccataac cttgttgaaa
      781 ttcatctctc aaaattgcat ttttttctgc ttttggcttc aactcaaatt caattccaga
      841 tttcatttca tcaaaataat ttaatactac aacctcatta ttcattttat tagttgtctt
      901 tataatccct ttagctccat ttacttcttc ttttgtaaat tcatcatcca atgttatatt
      961 aaatttgtct tcttttccat attcctcttg aaaatccctc ttcatttcat tgtatttagt
     1021 ttcaaactct ttactcttgt ttaattccat agctgataca tttgtcaatg atgctccaat
     1081 tgccattact gttaatgcca ttactatacc acttttaaat ttttttactt tcatatctta
     1141 cctcctaaat taaatgttct caatgaaatt atataaagaa aatcctaagt tttacagttt
     1201 cattcttgta actaaaatta ctattatgta accaaacttt ttgcttcaac tattccttaa
     1261 aagttgcttt tttgaggaat agttgaaatt aaataattag attttatcta tatttataga
     1321 tttaaaatca aatttatcca tataaataaa tacttatttt tgcaaaataa aaatttttaa
     1381 ttactccaat ccttcttatt tacttaaaat cctatgataa gaaatttaaa agtctttaca
     1441 ttctttaaca ataatttatt tacttttaat atacaaaata tagagagtat atcaaaataa
     1501 ttttactgaa tattctaaaa ataagtaccc cctattagta tatatattta catattaaaa
     1561 aaaagtttta aataaagtta tattttttac aacagttttg taattaaaaa catattgaag
//
LOCUS       DAFKMX010000335         1616 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFKMX010000335 DAFKMX010000000
VERSION     DAFKMX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.343"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            216..1556
                     /locus_tag="LZB08_003739"
     CDS             216..1556
                     /locus_tag="LZB08_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBM7317166.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGMGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          634 a          120 c          260 g          602 t
ORIGIN      
        1 agttatagat aaatttatat agttattatt aaaaaaagca ataaaaagtt taattcaatt
       61 tagcaaattt agtgatataa ttaaaaaaga aaagaaatat ggacttattt ggaataattt
      121 tgacaatcta tttactacat gtgtagagtc gtatataatt aaattataca taaaatataa
      181 aaaaataatt tagtaatacg ttaggagaaa gtattatgat aaagtcaagc ttattttgga
      241 atattttcat tgcgatatcc tttatgatag aatggtttct ttgtaagaaa atgctagatt
      301 atacaagtga gaaaaggatt tctaacaaaa aaataaactt ttgtatggta ggtattattg
      361 tttttgctga cataatatat ttttttgatg tacctccaaa tataagagta attatatcta
      421 tgataattac tattgcattt tataaaatct catatgatgt ggggtttata aagtctgtat
      481 taatatcctt aatttattgg atgttactaa tcgccacaga tgctttgagt atatctttaa
      541 cagtttggct taattctata aatgacatga gtagtatggg gatgggagat atatacagaa
      601 ttgagtctat aatattggga aagatacttt tgattgtgtc tttgctttta tatagagcct
      661 taaagataaa acttaatata gaaataaata aaaggacatt attttatata gttattccta
      721 tagtcgctaa tatagcatct ttttttgtaa tttttgaata catatttaat ccagggtctg
      781 gtagcttaat acataatttc caattcgtta tgatttctgt tgtattgctt atatcaagta
      841 tcactttagt tttaattgct aaaaaattac agcaatataa tactttagtt gtagaaaaca
      901 atatgattaa aaatactttg aagtatgaaa atgagtatta tgaagatata gaaaagaaat
      961 atttaaaaac tagattacta agtcatgata tgaaaaatca catattgtgt ataaatgcta
     1021 tgattaaaaa aggtattgat gttacttctt atttaaataa tttgcaggta gaaattcaga
     1081 gtaatgactt gttatttaat actggtaatt ttatattaga tgctatatta agtgaaaaga
     1141 aagaggtttg tgataagaat gatatagaac taaaaatagg aataaatttt agttgttgtg
     1201 aagctgttga attagtggat atatgtcata tattttcaaa tattttagac aatgcaattg
     1261 aggcatgtaa taaaattgat agtggaaata gagttatttg tgttaaaggt gatgttatgc
     1321 aaaattttta tttaataaga gttgaaaata ctaagatgaa taagattatt actaaaaatg
     1381 gagatatttt gacagacaaa aaaggttctc ttcatggact tggaataaaa agtgtaaaaa
     1441 actctgtaaa taagtatggt ggtaatgttg taattgaata tgatgataaa aaatttgtag
     1501 ttaaaattgc tattccactt agaaatgtga agaaagcagt taatcatgtg atttaaatgt
     1561 atataaattt gatgtgtaga aatttaatat ataagaatta atgtataaaa gtgatg
//
LOCUS       DAFKMX010000336         1592 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFKMX010000336 DAFKMX010000000
VERSION     DAFKMX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.344"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            119..592
                     /locus_tag="LZB08_003740"
     CDS             119..592
                     /locus_tag="LZB08_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7317167.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGIMTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(961..1518)
                     /locus_tag="LZB08_003741"
     CDS             complement(961..1518)
                     /locus_tag="LZB08_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBM7317168.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
BASE COUNT          585 a          211 c          168 g          628 t
ORIGIN      
        1 ctataatata aatcatatat tatcataata ttctaaattt ataaaacaaa ttgtattgaa
       61 ttgaatttat ttaagatata taattataat taaaataata ataaaggtgt tgtttataat
      121 gttcaaagaa tacttaataa gagaattaaa agaagatgat attaaaaatg cattagactt
      181 aatctggaac gtatttgaag aatttgaatc tccagattat tgtgatgaag gaattatgac
      241 atttcatcaa tttatagaat ataactctat tattgaaaaa atcaaagaaa aaactctctt
      301 cttttggggt tgttttgact ctaaaaaact tattggagtc ttagctatta gaaatttaaa
      361 tcatatatgt atgttatttg tagataaaaa ctatcataaa catggtgttg caaaaaaatt
      421 aatgactaca ggcatacaat actgtatttg taataaattt aatttaactg aaataactgt
      481 aaattcatca ccttatgctg ttgaagttta tcataaatta ggatttaaag atactgctgt
      541 tgagcaaatt gttgacggta tacgttttac acctatgaaa ttgttaatat aataaattaa
      601 ataaaaagca aagtctctta aatattttat agttactttg cttttaaacc aagatttaaa
      661 ataatactat cttttttgtt ttttaataat atactttaaa atccttacta atatgaacta
      721 taatttattt tttgatatcc tttttgatta acatataaat taattcaatt attaaacata
      781 aactatgttg tttctcttta tatctcttct ttgtaaacaa tattgggcat ttgaaaataa
      841 atattcttaa aatatactgt tctcctgttg attttccttt ggtacaaatg ataaaaattt
      901 aatttacatg ccccataatg aagtgttcaa taaataagtt aaaataattt atttacctcc
      961 ttaatcatat tttttatgaa tacctaataa cacagtatca atagaatcga ctaaaccttt
     1021 tatagcttct tctttactca aaaatgattt cccaagttgc aaagtcgaat aaccatgaag
     1081 tacactcatt attaagttta atacttcacc tgtattcttt gtatttagat tacaagaaag
     1141 aataagtttt tctaataagg atttatattc atcaaagatt tctaatgttt cattatttcc
     1201 atgccaagta gcccactgaa ttgtttcata aacaccagga ttagaaatta taaaattaaa
     1261 gtatgctata cttatacact ttatagctgt atctccaaaa tttccaatag ccgcctttat
     1321 catttgacta ttcatctctc tcattccttt atgagctact tcaagtaata aatcatctag
     1381 actttctata tgattgtaca aagatggtgt acgtattcct agcttttctg caataacttt
     1441 taaagaaact ttgttaagcc catctttatt aactatatct gatgtagctt gtataactat
     1501 ttctctacta acacgcatga ttttccctct ttcatttaaa attaattact ttattagttt
     1561 aactaatcta attagtttcg tcaattattg ta
//
LOCUS       DAFKMX010000337         1572 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.345, whole genome shotgun sequence.
ACCESSION   DAFKMX010000337 DAFKMX010000000
VERSION     DAFKMX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.345"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     misc_binding    462..725
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            876..1427
                     /locus_tag="LZB08_003742"
     CDS             876..1427
                     /locus_tag="LZB08_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBM7317169.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          643 a          152 c          240 g          537 t
ORIGIN      
        1 aaagaaagaa gaaaaaattt tactaaaaat aattaaaaga atatcatttt tttaatcatt
       61 ggtatctatt taataatatg atgtaattaa aatggagtaa tattctgttt caaaggaggc
      121 atataaatac aggaatgagt attaaatgat ggttaattaa gtaaatttat tgataaatgt
      181 acttaattaa ctttgaagaa tttaattcct catgaatcaa aatataaaat tacatcaaca
      241 gttattcaca aaattaatat aagaagaatt agagccaaag agcagactat tagaccatta
      301 aagtaattat tcatagaatt ttgtatatga aaagaaatgt atagtataat ttactttttt
      361 tataaaaaat agattacaaa atatattttt tataaaaagg tattgacatt ttttagaaaa
      421 aagaatatca ttattttaac aacaaaattc aatacaataa attaaatcct atgacaagga
      481 agagtagtta aatttaggtt cagtatagag agctgaggat ggtggaatct tagcaagaaa
      541 aaatttgatg aatggccctt agaggaataa attgaaatca tagttttgag agtagacttt
      601 atcgtcagtc gcacgttata gcgaataagg tatgtaagta cctgataatg agatgtatga
      661 aaattgaatt ttatattaat ttttatataa tttaggtggc aacgcggata atctccgtcc
      721 tattagttta ggacggagtt ttttatttta ctgaaaattt aaaagataga aggtggtgag
      781 aaagatgtta tgaatttcaa aagctaaaga tgatgatata gtaggttggc aaaaaaataa
      841 attgaaaatt aaaaaaatag gaggaagtac aaaaaatgaa aacaaataca aaaaaattaa
      901 ctttaaatgc aatactttta gcgatgggat tattaataca tcaaatcaca ccagctatag
      961 gattaccaat gaagccagat gtaccactag caatgttatt tgtaatattg gttttaaata
     1021 gagatgatta taaaacttgt ttgatagcgg gtatagtaac aggaatattt acagcactta
     1081 catcaagttt tccgggaggt caaattccta atgtaataga taaaactctt acagcaaata
     1141 tagtattttt attaatgaat attagttata atatgccttt tataaaaaaa ttagctaaga
     1201 aaacacaaga tactgtagta gttgcaataa taatgccaat aggaactctt gtaagtggaa
     1261 ctatcttttt attagcagct caagctatag ttggtcttcc aggggcttca tttacagctt
     1321 tattcttagc agtagtatta ccagctgttt taataaatct agttgcagga atatttttat
     1381 tcaagattgt tagtctatca ataagaagag ttagttatca agcttaaaca cgaaataata
     1441 ttttacttta taaagcatta taataactaa ataacataag aataaagtag attataacta
     1501 ttttattctt atgttgttta aagatttggt attatatatt tatttttaat aaaatattta
     1561 tatagtacca at
//
LOCUS       DAFKMX010000338         1558 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.346, whole genome shotgun sequence.
ACCESSION   DAFKMX010000338 DAFKMX010000000
VERSION     DAFKMX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.346"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            129..1493
                     /locus_tag="LZB08_003743"
     CDS             129..1493
                     /locus_tag="LZB08_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBM7317170.1"
                     /translation="MDIKELVKMQRKYYNTSKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKMM"
BASE COUNT          617 a          161 c          257 g          523 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaataat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaagta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg gattttgaag
      301 gttttatgac agaagtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccatttc
      481 aattagcact tataccatta gtttggtcat tagcagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagttgaa gatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggggataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcgaatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttgg atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggagga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagatgatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttatttt
//
LOCUS       DAFKMX010000339         1556 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.347, whole genome shotgun sequence.
ACCESSION   DAFKMX010000339 DAFKMX010000000
VERSION     DAFKMX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.347"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            189..1001
                     /locus_tag="LZB08_003744"
     CDS             189..1001
                     /locus_tag="LZB08_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBM7317171.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1090..1281)
                     /locus_tag="LZB08_003745"
     CDS             complement(1090..1281)
                     /locus_tag="LZB08_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317172.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          574 a          184 c          219 g          579 t
ORIGIN      
        1 tgaaataatc actttttcaa gaatgttata atttttttat tgtattcttt ttgctatagt
       61 ctatgaataa atcaatagaa aatcttctaa gttgctttta aataatgact ttatggttca
      121 attgtactaa aatagtatat gtaggttaca aataatctaa taaatattaa agtttggagg
      181 taatagatat ggcagattgt aattcatgtc catcaaaggg aaattgtaat agtcaatcaa
      241 attgttctat tgaaaataac cctaacaata aatttggaaa aataattggt gttatgagtg
      301 ggaagggtgg agttggaaaa tcaactgtaa ctgctttact tgctaataag ctaaacaaaa
      361 tgggatataa ggttggtata ttagactcag atataactgg accaagtatt cctagactta
      421 tgggtgtaaa aaatgtaaaa gcttattctg atggctctta tatttaccct gtagaaaact
      481 caaataacat aaaagtaatg tctataaacc ttatgataga tgatgaaaat gaacctgttg
      541 tttggagagg acctttactt ggaggggttg taaagcaatt ttatactgat gtactttggg
      601 aagaattaga ctatttatta atagatatgc ctcctggtac tggagatgtt gccttaacag
      661 ttatgcaatc tattcctata agtggaatag ttatggtttc agtacctcaa gatttggtat
      721 ctatgattgt atcaaaagct gttaatatgg ctaaaaaaat gaacataaat gtccttggag
      781 ttattgaaaa tatgagttat atccaatgtc ctgattgttc taaaaaaatc aagttatttg
      841 aaggagaatc tactgaaaag tttttagatg atttagactt agagctttta ggagaacttc
      901 ctatgactaa agaaattata gatattactc ataatggagt tacagaaatt agtgatgatt
      961 tagattcaat attgactaat gttgttgaaa aaattaaata aataaaaatt ttataatttt
     1021 atatttatca aaaaaagagg cttgtattta ttttataatc atacaaataa atataaacct
     1081 cttttatgtt tatctatgtt ttaaatacac aaaatattct tctacttcat ctccattttt
     1141 tacttttact aaatattcat tataatgagc attatatggc tgtgtcattt tgcattctat
     1201 tacattttct atgtcttcta attctaaaag ttctacatcc tttaaaaatt ttctttctat
     1261 gagttccatt aatctttcca taatatatcc tcacttcccc tataattaag ataaactata
     1321 tatcctctta atacagagtt tatctgttta ttatattata tccaatatat tacagtttct
     1381 aacatcattc ataaattatt ttaacaaata gagattgatt ttttaataaa tatgccttga
     1441 taaaatgaaa catagaccaa ctcttagttt tatgtaaaga aaacttaaag aaaactatag
     1501 attatattca caaaaattac tcagaagata taacagtaag taatatatgt aatgaa
//
LOCUS       DAFKMX010000340         1551 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFKMX010000340 DAFKMX010000000
VERSION     DAFKMX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.348"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            160..600
                     /locus_tag="LZB08_003746"
     CDS             160..600
                     /locus_tag="LZB08_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBM7317173.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(788..973)
                     /locus_tag="LZB08_003747"
     CDS             complement(788..973)
                     /locus_tag="LZB08_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317174.1"
                     /translation="MNKNSNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKK"
BASE COUNT          584 a          230 c          155 g          582 t
ORIGIN      
        1 tataaatatc atatttttct attagctaaa actaattact ttatatttct acgtattagt
       61 ataataatac ataaaaactt gaataatgca taaaaacttg acatactgtt gtcataatta
      121 ctttgctata attaaaagta aaaatggagg taaattctaa tgtatgaacg tatgcttgat
      181 aaaagcaata aaccctgttt gtctcaaata atagaatata taggtaaaga tggatatgca
      241 cgattgtgta agttggaaac ttacttgaaa actcaatata atttatcaag agaacttcga
      301 tttccttttg gaaacaatta tggttggggt tacaaatata gccataaatc ttttcattta
      361 ttttatgctt tttttgaaaa aggtgcattt actgttctga ttcagatagg tgacaagcaa
      421 gttccagcag tagaaaaaac gttggtagct ttgacaccaa aagctaaaga gctctgggtc
      481 actcgttatc cttgtggcaa taatggaggt tggattaagt atcgtatttt agaagatgca
      541 gacttacttg atattattca atatataaga gcaaaaaaat ctccacctaa acaaaattaa
      601 cttttatttt ttgtgattta aatatagtgt atataaattc cattctttcc atataaaaaa
      661 aataatataa aaaatatcta ttagtataac ttatttagta tgtcttattc tactttttac
      721 agataattaa aaagttttat cataaataaa tcataactag acatcctagt aaatcaaaat
      781 taatttatta ttttttatta gaatttttga taatagtccc tatacatatg ccaattatca
      841 ttccaaaaat tagaccataa cacattgtga tacctacatt tcctttagaa atacctatag
      901 cacaacctat agccattcca agtatcatgc ctataaacat acatataata actaaattgc
      961 tgtttttatt catagttttt ctccattcaa tatattttaa attgcctagc tcgccttctt
     1021 agcttaaaac aaagaaatat aaattaactt tctttcattc taatatctgc ttgtataaat
     1081 gtcaaacaac acatttcttg tcataacctc ctctttttga agtaagtata atctcccaga
     1141 cacaataata tcaatttcct aaaaccattt aagtaacttt tatatttaca taatatttac
     1201 aatcataaac tatattgtta ttcatttata catcataaac actccctcct aatcattctt
     1261 atacttaaaa taatgttcat ttataataat atatctaacg cactttaatt gaaaaaacta
     1321 aaactttgac tttcattccc atatataaat aagtatactt tttgatgctc tattacaaat
     1381 caactaaatt aattgatata aattatcatt ttctgttgaa aataccattc gcacatgcta
     1441 tacttaatta cataaatggt aataaatgtc atatgatttt acaaatctat taataacaat
     1501 tattactttt actgtatttt aaattttttg gactaaatat caagaacttt c
//
LOCUS       DAFKMX010000341         1548 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.349, whole genome shotgun sequence.
ACCESSION   DAFKMX010000341 DAFKMX010000000
VERSION     DAFKMX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.349"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            180..1418
                     /locus_tag="LZB08_003748"
     CDS             180..1418
                     /locus_tag="LZB08_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBM7317175.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          662 a          135 c          259 g          492 t
ORIGIN      
        1 aattaatatg acataatata tttataaaat taagaatata aatagatatt ttaagggatt
       61 agttgttaaa ttaggaaata ttaaaaaaaa tatatattta tatttaaact attttgttat
      121 ttatataaaa atacttatct agttactagc aattattagt agaaatgggg gaaatagaga
      181 tgaataaggg attttacaaa ttaaattcag tggtattgaa ctttgatatt aagaatgatg
      241 ttaacaagat aagatttaat cattatataa tgagaaaaga aaacatacaa agacttcaag
      301 gaactctgcc aaacggacaa ttttatatga cagtgagaag agctgctaca gatttacaac
      361 tttctatttc tactataagt aggcttgtaa aagagttttt ggatttaggg ataataagat
      421 tagtgtctaa gggtgtaaaa ggtaatcgtt ctgtatatag ttacgtaagt atagagttta
      481 atggaagttt atttgatgat agcaaatata tggaacttga ctgtgtatgt aatgatgaat
      541 ttattgataa tagtggcata gagtttgatt gtgatagcaa agataattct tttaatggag
      601 atataaatgt agaaactgtg cattgcatta atgagtattt gtctgatgat gatgtagagc
      661 ataattataa caatttccat aacaaagata ctataaagga taaagccatt tctaatatta
      721 gcactatcaa aaaatatatt gatgaatctt ttgatacaaa taaatcagaa aattctaact
      781 tacaacagga gtcaataaat ttcaacttta atgatacatg caatgaaata gataaaagaa
      841 cgaataatag tatagaaaat aatcaaaata aaaggcctat gagattgagt gacatcagtc
      901 ttttaagtga taaaggtatg acaaataaaa agaattactt taatcataat catggtatac
      961 aaaaagggac taaatctgta acaaagaaaa aagaatcctt aaataaattt aataaaaaaa
     1021 atatacagca aagcattata aatatgctaa ataaaaaatc aggtaagaat tttaaaatcg
     1081 attctccaat aagcataaaa cttatcaatg aaaggctaaa ggaaggctat gtcttagaag
     1141 atttttacaa agttattgag gtaaaagtgg ctaagtggaa aaataccata atggaaatgt
     1201 atataagacc agaaactttg tttagctata agtttgaatc ctatgtaaat gaaaacatat
     1261 ctatgaaaag tcaaagtaat gttcacccat gtaaagaagg caatagtaat gatagcagta
     1321 attatataca ttctgattat acaacatatg atggcagtgt gcgtgagaga aaaaaatatg
     1381 agaatgtaag atactggaat cctatatgtg aaatttaatt agtttaagaa tgtatttatg
     1441 ataaataaga tattaataga gaatagtgag ctatagcaaa tgatatatca atagttggta
     1501 gtaagttata ttaagcaaga tattactatc aggtagtgaa ttgtattg
//
LOCUS       DAFKMX010000342         1516 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFKMX010000342 DAFKMX010000000
VERSION     DAFKMX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.350"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            169..978
                     /locus_tag="LZB08_003749"
     CDS             169..978
                     /locus_tag="LZB08_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBM7317176.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1123..1362
                     /locus_tag="LZB08_003750"
     CDS             1123..1362
                     /locus_tag="LZB08_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317177.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
BASE COUNT          645 a          102 c          257 g          512 t
ORIGIN      
        1 tatacctaat attacaatat aaaaatattt taataattaa ttattaggat gtttattttc
       61 ctatccttgt tttaaggatt tttttagtat attctaatac tatagtatta tagagtaata
      121 atgttgattt aaaataaaaa taagatttcg gaggagttta ttatataaat gaaagattac
      181 tataaaatag gagagatatc taaaatatat ggaataggta gagattcatt aatgtattat
      241 gaagaaatag gcattctaag accagttaga gatataaatg gatatagaat gtataatata
      301 tcagatatat ggaaattaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag
      361 aaaataaaag aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa
      421 gaactcgatt taattgataa aaaaatagct gaatttataa gccataagga aaatattata
      481 aaaagattga gttcaattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt
      541 gtatatatag aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac
      601 ttcgattttt taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga
      661 aataacaata ttggttctgc cttttctact gaagcaatta ataaaggtat atttaatgag
      721 tttaaatctg tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat
      781 gaaggatatt atgtaacttt aaattatact gggagtaatt ctaataataa gatgtatatg
      841 gagaaggtgt ttaaatttat agaagaaaac aattataaga taattgctga ccctatagaa
      901 atctacaaga tagatataca tgagactgga attgtagagg agtttgtaac ggaaattcaa
      961 gttcctataa ctaaatagct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg
     1021 ttgatttgcc accgatttgc cactgtgtta taaaatcggt agcaaattat attttaaatt
     1081 ataagatagc atgaaaaaat aattgtttaa ggggttataa atgtgaaatg ttctaattgt
     1141 ggaagtgtaa atattgaaaa gggaatttta acaactggtg gcatatatgc tgaaactatt
     1201 ggattaaaat atagaggaga tggaaaatta gataaagtta taaataaaac ttttccagta
     1261 caatctacta tatattcaga tttatgttta gattgtggcg aaatagttag aacctatata
     1321 aaggataata ctgataggaa ttggtgtaaa gataagaaat agaataattt ttgaagtata
     1381 ttaatatttg aagttgaatt ttattaaaga tacaagtgaa aaagtataaa tgggaagaaa
     1441 ttgttatatg gaagaaactt tgcatatatg gaagattagt tttttaatat aataacattt
     1501 ttaaatgtag ataaaa
//
LOCUS       DAFKMX010000343         1387 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.352, whole genome shotgun sequence.
ACCESSION   DAFKMX010000343 DAFKMX010000000
VERSION     DAFKMX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.352"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..528)
                     /locus_tag="LZB08_003751"
     CDS             complement(<1..528)
                     /locus_tag="LZB08_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBM7317178.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEK"
     gene            689..1342
                     /locus_tag="LZB08_003752"
     CDS             689..1342
                     /locus_tag="LZB08_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBM7317179.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
BASE COUNT          462 a          163 c          189 g          573 t
ORIGIN      
        1 tttttctgaa atatttttta attggtcagc cattgaagtc agacttaata aggctatcaa
       61 taaatcatct ttattttctg gatttatact aatattttca aaattagact ctataaagtc
      121 cggtatattc tttctttgat tttcttttat taacatgaat ttttcgtaac atgaaactaa
      181 atctttttca ctgaaattat ccttttcata cacaccatgt aagattcttt taatttcttg
      241 tattgtaagt gtatttttca tagaataaat aattatcatc tgtaaaattt gttctttagt
      301 atactttttt cccttaactg gtttgattaa tccctctttg ctgtaattat ttatcatggt
      361 tttagtaaga attttatcac tttcaaatct tttattagca gaaagcttat tgtcaattag
      421 agaaataatt tgatccatat ataagtcaat agatggaata tcgtttgaag ttatatctgc
      481 attatttagt gtttctagta ttatagtatt aagttcatta ttcatcatat gttacctcca
      541 taatatatat aatacactaa attagaaagt aaaacaatat taaggcttgt aaaaaaaaca
      601 taaagtggta taattaaata tataatatag taatgaaaac tatatttatg aatctgattt
      661 ttaaaataca taattgtagg aggacgaaat gtatcaattt tttttaaagg gtagagaccc
      721 aataagcagt ttaactcatt ttataggtgc ttgtttatct cttttagcaa ctataatttt
      781 agtttttcaa tctgttacat tacaggaaac ttctctcttg atgatagtat ctgtatcggt
      841 atttggactt tcactaattg ctttatatag tgcaagttcg tattatcatt ttttaaaggg
      901 gacaccagaa caagaattgt tttttagaaa agtagaccat gcaatgattt acgtattgat
      961 tgctggctct tatacaccaa tatgtctgaa ttttatggaa aaaaaagaag gcataatatt
     1021 tgttacagca atatggatag ttgcatttat tggtataata ataaaaatat tttggatgga
     1081 tgcacctaga tggttatcta ccagtatata tttacttatg ggatgggcaa ttgtttttga
     1141 tataaatgca ttcaattcaa ttcctaagga ttgccttaga ctattaatta tggaaggtgt
     1201 atcttactct ataggtgcaa ttatttatat aattaaaaag cctaatataa gtcctgagtt
     1261 tggatttcat gaaatattcc atatttttat tatgataggt tctttatttc actttttagc
     1321 agttttattg tatgttttat agttttaaaa gatttgatta attgatacta aaattctaca
     1381 atgaata
//
LOCUS       DAFKMX010000344         1359 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.353, whole genome shotgun sequence.
ACCESSION   DAFKMX010000344 DAFKMX010000000
VERSION     DAFKMX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.353"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            217..1314
                     /gene="ychF"
                     /locus_tag="LZB08_003753"
     CDS             217..1314
                     /gene="ychF"
                     /locus_tag="LZB08_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBM7317180.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          526 a          150 c          241 g          442 t
ORIGIN      
        1 atccttttta ttattttcta aaagcaatat ttttatttaa attaaataca aatattattt
       61 aaatcaatat gctttaaagt ctagtttaca agcttgatat atatgatgta taatcaatat
      121 gtatgtctat aattaaaaca attttgcaat cctatagatt gattgtttaa agttatataa
      181 agaatataaa aaagaataag agagaggacg attaatatga aattaggtat agttggttta
      241 ccaaatgtag gtaaaagcac actatttaac gcaataactc aagcaggtgc agaatctgca
      301 aattaccctt tttgtacaat agacccaaat gtgggagttg tatctgttcc agatgaaaga
      361 ttaaacaaac tacaagaatt atataattct gaaaaaatag ttcctactgc tatagaattt
      421 tgtgatatag caggtttagt tagaggagct tctaaaggag aaggtttagg aaataaattc
      481 ttatcacata taagagaagt tgatgcaata gttcatgttg ttagatgttt tgaggatgaa
      541 aatgttgtac atgtagatgg ttctgtagac ccacttcgtg atatagaaac tataaactta
      601 gaattaattt tctcagatat agagattctt gaaagaagaa ttaataaaac tcaaaaagct
      661 gctaaggctg ataaaacttt aggttctgaa cttgatttat taaaatcaat aatgtctact
      721 ttagaagaaa gtaagtgtgt aagaactatg gaatttacag aagatgaaca aacattcgta
      781 aattcattgg atttacttac atctaagcct gtaatatatg cttcaaatgt atcagaagat
      841 gatttagctg ataatggaga aaataataaa tatgttcaac aagttaaagc atttgcagaa
      901 acagaagatg ccgaagttgt tgtagtatgt gcacaaatag aagcagaaat ttctgaactt
      961 gattctgctg aagaaaagaa agagttttta gaaacattag gtcttgaaca atcagggctt
     1021 gataagctta taaaatcttc ttatgcttta cttggtttaa tttcattctt aactgctggt
     1081 ccaaaagaag ttagagcatg gactataaaa gttggaagta aagctcctca ggcaggagga
     1141 aaaatacatt ctgatataga aagaggattt atcagagcag aaacaatagc ctttaatgat
     1201 ttggttgagc atggtactat ggctgctgct aaagaaaaag gattggttaa attagaaggt
     1261 aaagaataca tagttaaaga tggagatgta atattattta gatttaatgt ttaaaatgat
     1321 taatcgtata cactttgact attatatata atcactaaa
//
LOCUS       DAFKMX010000345         1357 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.354, whole genome shotgun sequence.
ACCESSION   DAFKMX010000345 DAFKMX010000000
VERSION     DAFKMX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.354"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            250..>1357
                     /locus_tag="LZB08_003754"
     CDS             250..>1357
                     /locus_tag="LZB08_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBM7317181.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIK"
BASE COUNT          571 a          169 c          156 g          461 t
ORIGIN      
        1 tttcactaat aatatattaa caagttaact taatgtagat tattctttcc catttgttat
       61 aataatataa tataaagagc taataaagtt attttaataa catttaacca gttttttatg
      121 taggtaacat ttctacattt ttataaattt ttttacgttt tttaatttta taattaaaaa
      181 tttaaatttt atacatatta taataaattg tgatatatat aaaataatta aagataaaag
      241 gagtatttta tgaacatact tggattaata gttgaatata acccatttca taatggacac
      301 atatatcatt tatctaaatc tatagaaaaa acaaatgcaa cacatactgt agcaataatg
      361 agtggaaatt ttctacaaag aggcgaacca gctttatttg ataaatacac tagagcaaaa
      421 attgctgtcg aaaatggtgt agacttagtt atagaattac caactttatt tgcttgtcaa
      481 agtgctgaaa ttttttctca tggtgctata acaattttaa attcactaaa ctgcataaat
      541 tctatatgtt ttggtagtga agaaggaaat acagaaattc tttataatat agctgaaatt
      601 ttagcaaatg aaccaaatga atttaagtta cttttaaaaa aatatttaga tgatggtctg
      661 ttatttccaa cagctagagc taatgcactt tttgattata tacatcaaga caaaaaaaat
      721 aattttatac aaaattattc aaaggaacaa ttatcaaaaa cattaaattc atcaaacaac
      781 atactgggta tagaatacat aaaaaacctc atccatttaa acagtagtat aaaaccattt
      841 actatctcca gggtccaatc agaatataat tccgaagaaa ttgagagtga tatttgttct
      901 gctactgcta taagaacttc acttaaagaa aatacagacc tttttcattt aaaaaatgtt
      961 attccagaaa aaacttataa catcctaaat gaaaaaatag ataatggttt tttacctatg
     1021 tttgatgact tcttctttga tattttaaaa acaattatac ttagagatat ggacacttta
     1081 aaagagtatt ttgaagtaaa tgaaggcata gaaaacagaa tctataaaga tatctttaca
     1141 tctgaatttc tacatgaact acaattagca attaaatcca agcgatacac tttaaccaaa
     1201 atcaaacgaa ctctaaacaa tatattgcta ggtattaaaa aaagcgacat gcaacttata
     1261 aaaaacatag atactatacc ttatataaga atacttgctt ttaatgataa aggtagagaa
     1321 attctaaaag agataaaaaa taaatctgaa ataaaaa
//
LOCUS       DAFKMX010000346         1329 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFKMX010000346 DAFKMX010000000
VERSION     DAFKMX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.355"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            52..783
                     /locus_tag="LZB08_003755"
     CDS             52..783
                     /locus_tag="LZB08_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317182.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(940..1146)
                     /locus_tag="LZB08_003756"
     CDS             complement(940..1146)
                     /locus_tag="LZB08_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317183.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          555 a          137 c          154 g          483 t
ORIGIN      
        1 acaaatttat ttttcacaag tacaatactt atttaaaaaa ggaggtttaa tatgttaagt
       61 aaaaaaaatg taagtttaat tattttagta ataattttaa gtttattaac tgctatattt
      121 attttcaata ataaaaatac taacaataaa ttgaaagaag ttactaagaa taatgaagaa
      181 atagtaccta aaaacttaga aggtttaagt atagaacaat taatagagat tagagataaa
      241 gccattgata aaaatttaga aggtcttagg gaatttggag aagattcagt aaaaaaacgt
      301 aaagattcaa ttttaaactt tgaatttgga ttagtagctt ttgttaacaa gaattatagc
      361 aacttaacag aaaaagagaa attaataaga caaattaata ttgttaacta tgaaaactct
      421 ataaatgata taatggatcc ttcaggttca ttctttgaat tagcaaatca gaattaccaa
      481 gtgaatgagt ttctgttaaa aaacggaaaa gattataaag aatggtcata tgaaggtcta
      541 gattttctaa ttgatcatct agaagaactg tgcaaatttg ttgattttga tgtaaaagat
      601 gtaatagata ttattgatcc tacaataaaa acagatcttt cagaggaaca aaagaaatct
      661 ttgaatgata aattaaagaa aatatcttca agtgatactt taagtgaaga agttaaaaag
      721 gagataaaaa actgggaaaa caatttaaat aatttaaata tgaataaaaa taattctaat
      781 taattttctc ataagaaaaa tgcttatatc gtgataatgt gaaatataca taattataaa
      841 caaaaaaggg gctgtgacac taaaaaatag tgctacgccc ttttttattt gtaataatca
      901 tcaatacaaa ttattttatt atatgtaatt atttatgtct taaccaattt tatcgaaaat
      961 aacagtcttc tcttttaaag agatttttcc tacttgatat ccatattcat ttaattcctt
     1021 tttatatttc aaaaacgaat ggtctatctc tatcccagca atatctttta tttcttcaaa
     1081 agttaattta aacgattgac tattgttttt cttaacatat tcccataaag aactatattt
     1141 actcatctta tatcactcct tttaaaatca tatcttatct attaatttat attctatcat
     1201 tattaatgta taaagtcatg ctacttatta tttatatata aaaatatcca aataataagt
     1261 agcaattttt tatagtcata atattttttt cagattaaat aaaaatcaat tatagttaaa
     1321 atattttta
//
LOCUS       DAFKMX010000347         1324 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.356, whole genome shotgun sequence.
ACCESSION   DAFKMX010000347 DAFKMX010000000
VERSION     DAFKMX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.356"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            165..875
                     /gene="rgaR"
                     /locus_tag="LZB08_003757"
     CDS             165..875
                     /gene="rgaR"
                     /locus_tag="LZB08_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBM7317184.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          527 a          109 c          206 g          482 t
ORIGIN      
        1 aggaatttat cgtaatttaa gtttaaatat taaagagaat ttctataagt agtttaatcg
       61 acaatttaaa tttataaaga aatttaaatt tgaaagctag tgagtacagt ttggaaattg
      121 aaataaagta aactaaatga tattttagat ttggaggtga tattatgttc agggtagtta
      181 tatgtgatga cgaaaaaata caaagaagca tactaaaaga gtttacagaa aaacttttgt
      241 ctgaaagatg tctaaattat caaatactgg aattttcctg tggagaagat ttaatatcca
      301 aatacccaga aaaaattgat attatatttt tagatatcca aatgaaagat atcaatggaa
      361 ttgaggtggc taaaaaaata agaaagtttg atgaaaaagt agaaattata tttacaacgg
      421 ctttttcaga gtatgccccc caaggttatg aagttcgtgc ttatagatat ttagtaaagc
      481 ctatagagta tagtaacttt gcaatgggtg taaatctttg tattgataac cttcaaagaa
      541 agaaggagcg ttatatagtt ttaaatagta aaaaaggatt taatagaata ctgatagatt
      601 ctattttata tgttgaaact gtaaagagag atttagttgt acatacaata ggtagagatt
      661 ataaagctaa tatgagcatg aaacaagcag aaaaattact gtgtgaaaat ggctttttta
      721 gatgtcatac aagttttatt gttaatttaa gaagaataga agatcttagg gataatacga
      781 ttactattaa tgaacaattt atacctataa gtaagtatag acttaaagat ttaaaagtag
      841 cattaacaag cttgttatgt gatataatgt attaatttta aaaaggtaat tgaaaatatc
      901 gtaaaaaaga tttaatgtca tcacagggct attgattgaa actgtcttaa tatataaaat
      961 gttatttata ttaagacagt tttattatgc aaaatagcag tttatagatt tttaatatgg
     1021 tttaatatgt atatgtagag tttccattat aatagcatga ttattaatta gtagcttgat
     1081 tatcaatata ctattattga tttttatatg cattatatgt atttttagaa tatgattacc
     1141 tttttaaaat aatgactatt tataaaactc tatagatatt tgtttcattt agctacgcat
     1201 actttaatta attatattaa tatttaatgg agtacaatat aaatattaaa gcgacatctt
     1261 atagtttatt gaacctatag taatgaatta ataaatagtt taattctatg aaaagatttc
     1321 cttt
//
LOCUS       DAFKMX010000348         1317 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFKMX010000348 DAFKMX010000000
VERSION     DAFKMX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.357"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            138..623
                     /locus_tag="LZB08_003758"
     CDS             138..623
                     /locus_tag="LZB08_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBM7317185.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            979..>1317
                     /locus_tag="LZB08_003759"
     CDS             979..>1317
                     /locus_tag="LZB08_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317186.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIF"
BASE COUNT          608 a          108 c          186 g          415 t
ORIGIN      
        1 aactataggg actaaaatct ttatgtaata aaggtcatgt cagccagttg ccaaaaagat
       61 aataattctt tttgttaaaa gtatttttag agttatatat tttaataact taatatttgg
      121 caagaggagg gcttaagatg atatctgaat taatgttaaa aaattttcgc aaatctagca
      181 tcaattttaa gactaaaatt aaattgaatt taagagaaaa accagacata aatagtttga
      241 aattaaaaag tataccagaa ggtaaaattg ttaagttaaa gtgtgtagat ggaatatggg
      301 cagaagttga gagtaattat gacaaaggtt ggttacttta taaatattta gaaagattaa
      361 gtaatgctaa aaacaatagt aatgatacaa taaaaaatag aaaaaagaat tacataggaa
      421 atataagaac aaatggatta agtttagaac ttagaaatga tagaacatta gaatctaaag
      481 ttataactac aatccctgat ggttttaaag ttgaagtatg ttatagcgtt ggaaaatggg
      541 caagagttaa tatcaataaa aatgaaaaac gatattctgg atatgtatat aatcaataca
      601 tagaagagca tttaagttta taatacgata ctagcaagct ttaaatatat taataaatcg
      661 caaaaaagaa tcccattaat gggattcttt tttgtagaaa cattataaca gaactaaaga
      721 gaataaatta actaatttat ttttattata aagtaattat aaatggtaaa tttaattgaa
      781 agtagattta acttatactt tatacaccaa gtatcataaa ttaatactac tttgaaaaat
      841 cttaaaaaaa gtaaaagtta tgataataaa aataatatta aagataataa gatatataag
      901 aaacttgaaa aaatttgtaa tttttagtta tatatatatt ataatgaact agttaaaaat
      961 ttttatgagg gggaaaatat gaataagaag aaaaaattga ttgggatagc tatatctttt
     1021 ctacttataa tcagcattat aaatccatta caagtagaag cagaagctat tattgagtct
     1081 agtgtataca tttcttcaat aaaagagaat tatacatatg ataatactaa atttaaaact
     1141 attaaatcta aaagtagaca taaaagtagg aaatataata aaagtaaagt aaagttgaaa
     1201 aaacgaagta taagaactaa aaagtatgga tatcaaacta taaataaata taaaagacaa
     1261 aacgcaagaa gacataatat cctaacaaaa ttaatattgt ccataatagt catattt
//
LOCUS       DAFKMX010000349         1219 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.359, whole genome shotgun sequence.
ACCESSION   DAFKMX010000349 DAFKMX010000000
VERSION     DAFKMX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.359"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            192..1181
                     /locus_tag="LZB08_003760"
     CDS             192..1181
                     /locus_tag="LZB08_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBM7317187.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          457 a          123 c          230 g          409 t
ORIGIN      
        1 catatatcat atgatttatt tggaaatcaa tattaataca ttaaaataac caaatacatg
       61 tattcataaa gatgtataaa aaatatataa acatatagta ttaattttaa attcaacaat
      121 ttaattgtta aaagtgttat tattaaatta gagatatctt aacttaatat actgagatgg
      181 gagtgataga tatgttaaaa gaagaaaaaa cttatcaaga gaaagacgca aaagtattca
      241 atggagtggc agctttgata atgattttat tatttttagt agtttcaata ggtatcatgg
      301 tttttgggat agtaaaactt gatagtggat ttactggaat aggttcactt atgttaattt
      361 tgggacttgt atttatagta gtagatttta tcctattttt tgggctaaaa atgattaatc
      421 ctaaagaagc tatagttcta gttttgtttg gtaactatta tggaactata aaaaaagaag
      481 ggtactattg ggtgaatcca ttttgttcag caataaaccc agcagtatca agaattaatg
      541 tatcaaaatc ttcatctgat gaaaaggtag atatatcaac taattcaagg ggcaaaaaag
      601 tatctttaaa aacaatgact cttaataatg aaaagcagaa agtcaatgat ttattaggaa
      661 atccaattat aataggagtg gttgtaattt ggaaggttat agatgcaaca aaggcagtat
      721 ttaatgtgga taattataat acattcttat ctattcagtg tgattctact attcgtaatg
      781 tttcaagact ttatccatat gatgtatctg aagatggaga tgaaaaatct cttcgtggta
      841 gtagtcagga aatagcagat agattaaaag atgaattaca atcaagggtt gatattgcag
      901 gaattgaagt gtgtgaagtg agaattactc atctttctta tgcaccagag attgcagcag
      961 caatgttaca acgtcaacaa gcagaggcta taatagcagc acgtaaaaag attgttgagg
     1021 gggctgtaag tatggtagag atggcattga ctaatttaag tgaaaataat gttgtgactt
     1081 tggacgaaga acgtaaagca gctatggtaa gtaatctgtt agttgtgctt tgtggcaata
     1141 aagatgcaca accagtggta aacagcagtt ctatttatta aattataaaa agtttaatag
     1201 tttattttat tgatatata
//
LOCUS       DAFKMX010000350         1210 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.360, whole genome shotgun sequence.
ACCESSION   DAFKMX010000350 DAFKMX010000000
VERSION     DAFKMX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.360"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            491..958
                     /locus_tag="LZB08_003761"
     CDS             491..958
                     /locus_tag="LZB08_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBM7317188.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          481 a           79 c          184 g          466 t
ORIGIN      
        1 ttaaagagta aatataatta ttagtaatat atatagtatt tgtaacaaaa tatcaataat
       61 tagaaagatt atgaaaacta aattagaaga actaaaaact atatttttat taattagaat
      121 atttgaataa tctctttaat catataaaat gcatatactt gtaatattta aaggcgatat
      181 taatattatt agtgtatatt aatattccct tgaaattaaa aaaacataga tatttcagta
      241 atctaaaata gtctacaaaa ataatagttt tatcaaaatt aataagaata catatagatt
      301 taaacctaaa agtatgaaca tgtctgtttt ttacaagatt aaaatttata ttatatgata
      361 aaattaaatt aacttaacta tttcaataag acttataatt tttttcttta tactagacct
      421 tattgaaacc tttatagttt aattctaatt atatattata aataaagtat gtgtatatta
      481 aggaggtaat atgaagtttt taaatgtttt aatagtagtt gaagatattg agaaatcaaa
      541 aaagttttat tatgatgttt tggggttaaa ggtaatctgt gattttggag aaaatgttgt
      601 attagaaggt aatatatcat tacaagaaaa aaagctttgg ttagaattta ttaataagag
      661 tgacagtgaa gttaagttca atggaaatga tgctgagtta tattttgagg aagataattt
      721 tgatactttt gttgaaagat tgagtacaat gaaagatata gattatgttc atctagctat
      781 agagcatagg tggggtcaaa gagcaattag attttatgat ttagatgggc atattattga
      841 ggttggagaa actatgtcat ctgtgtgtag acgtttttta gatagtggtc tttcaataga
      901 tgaggtagct aaaagaatgg atgtaactgt cgaatatatt gaatcagttt tagaatagat
      961 ggattatatt aaagtaaaac tatattttgt atagaagtaa gggctgtaag taaacttgta
     1021 tggaaacaaa tttacttaca gtttttgttt ttgtaagata cagaatataa tatttgttat
     1081 aattttattt ggatttttaa aaagtgcatg attttcctta catattataa tagaatttaa
     1141 tatttattgg atgatttaaa tttagaaaaa tataagtatt agcttataaa agataattat
     1201 acttactatc
//
LOCUS       DAFKMX010000351         1207 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.361, whole genome shotgun sequence.
ACCESSION   DAFKMX010000351 DAFKMX010000000
VERSION     DAFKMX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.361"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..>1207
                     /locus_tag="LZB08_003762"
     rRNA            <1..>1207
                     /locus_tag="LZB08_003762"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          319 a          265 c          361 g          262 t
ORIGIN      
        1 ctaagcacac tgttggttat gtgtgcccaa gcattgaggc agtcaaagta ggcaaatccg
       61 ctttgataat gctgggatgt gatggggagc gaaatttagt agcgaagtag ctgatttcac
      121 actgtcaaga aaagtctcta tcgaggttaa aggtacccgt accgcaaacc gacacaggtg
      181 ggtgaggaga gtatcctaag gccagcgaga gaactgttgt taaggaactc ggcaaaatga
      241 ccccgtaact tagggataag gggtgccacc atcaggtggc cgcagagaat aggcccaagc
      301 gactgtttac caaaaacata ggtttctgct aagtcgcaag acgatgtata ggagctgacg
      361 cctgcccggt gctggaaggt taaggggatc tgttagagca atcgaagcag tgaacttaag
      421 ccccagtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt
      481 aagttccgac ccgcacgaaa ggcgtaacga tttgggcact gtctcaacaa cagactcggt
      541 gaaattgtaa ttccggtgaa gatgccggat acctgcgaca ggacggaaag accccatgga
      601 gctttactgt agcttgacat tgggtcttgg tactacatgt acaggatagg tgggaggctt
      661 tgaaaccagg acgccagttt tggcggagcc atccttggga taccaccctt gtagtactgg
      721 gactctaacc ataggccatg aatctggtct tgggacactg tcaggtgggc agtttgactg
      781 gggcggtcgc ctcccaaaag gtaacggagg cgctcaaagg ttctctcagt acggtcggaa
      841 atcgtacgta gagtgtaaag gcaaaagaga gcttgattgc aagacataca ggtcgagcaa
      901 ggatgaaaat cggacttagt gatccggtgg ttctgcgtgg aagggccatc gctcaacgga
      961 taaaagctac cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg
     1021 tttggcacct cgatgtcggc tcatcacatc ctggggctgt agtaggtccc aagggttggg
     1081 ctgttcgccc attaaagtgg tacgcgagct gggttcagaa cgtcgtgaga cagttcggtc
     1141 cctatccgtc gcaggcgtag gaaatttgag aagacctgtc cttagtacga gaggaccggg
     1201 atggacg
//
LOCUS       DAFKMX010000352         1203 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFKMX010000352 DAFKMX010000000
VERSION     DAFKMX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.362"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            91..1131
                     /locus_tag="LZB08_003763"
     CDS             91..1131
                     /locus_tag="LZB08_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBM7317189.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          467 a          142 c          195 g          399 t
ORIGIN      
        1 gtttagattc tattctttta attttagttt tcagaacatt tgtatttatg ttataatttg
       61 tataaatata tttatttagg aggaaacaat atgaatttaa atttaactaa tactataaat
      121 ttaatgaaaa aatatttgga cattccaagc ccagctggat atactgaaaa tgcagtttta
      181 gaaatcaaaa aagattttga aaatttaggt cttgacacta ttcttacaaa aaaaggtgca
      241 cttatagcta ctcttcctgg tgaagatgat tcaaatcaaa taacaatatc tgcacatatg
      301 gatacacttg gagctatagt aaaagaaata tcatctgatg gtactcttaa ataccataaa
      361 gttggtggtg gatgttgggc agctatagag ggagaaaact gtactgttat aactagaaaa
      421 ggtaaaaaaa tcagaggaag tgttgtattt aagtatgctt ctactcatat atatggtcaa
      481 acaaaagctg ccactgaaag aaatgaagaa acaatgatta ttagacttga tgaaaaagta
      541 tttacaaagc aagatgtatt agatttagga ataagtgttg gggattttgt atgcctagac
      601 catagatttg aggctacaga atctggtttt ataaaatcta gatatataga taataaatca
      661 gctgtagcaa tggttcttga aatatgtaga tattttaaag aaaacaattt aacacctaag
      721 catactacta acttttatat aagtaattat gaagaaattg gacatggtgt ttctaaatgt
      781 ttacctgaaa aaacaaaaga atttgtagca atagatatag cacctactgg accaagtcaa
      841 acatctagtg aacatggtgt aactatagtt gcaaaagata atttatcatt atatgatttt
      901 aatttaagaa acaagcttgt cgatgttgca gaagacaata atttagacta cgctgtcgat
      961 gtatttactt tctatggttc agatgcttca gaagctattc gttggggaga agatgttcaa
     1021 atagcatgtg ttggtcctgg aacaaacaat agtcaccatt atgaaagaac acatatagaa
     1081 gctattgaaa atacactaaa attacttata aattatatgc ttaaagaata attaattata
     1141 tattttacca aagtcaattc ttaacaaata attaacttta atttttattt ttatacaaaa
     1201 att
//
LOCUS       DAFKMX010000353         1188 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.363, whole genome shotgun sequence.
ACCESSION   DAFKMX010000353 DAFKMX010000000
VERSION     DAFKMX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.363"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            4..261
                     /locus_tag="LZB08_003764"
     CDS             4..261
                     /locus_tag="LZB08_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317190.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(371..1051)
                     /locus_tag="LZB08_003765"
     CDS             complement(371..1051)
                     /locus_tag="LZB08_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBM7317191.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          488 a          148 c          127 g          425 t
ORIGIN      
        1 gatatgataa taattagaag tcaagataaa actaatttaa tgcagataaa ccaaattaaa
       61 atagatggta gtcaagttta tgcagtattt gaaagtaaaa ttgatactgt aaaaatagga
      121 gattatgaaa ataatacaag agctatacaa gtgttggaca atatacaaaa cttcattgaa
      181 aatggaacaa aatatgatta tataacatca aataaagtca gatacaatgt taataaaata
      241 tttcagatgc cagctaaata gttagtgtgc attttaattt aataactaaa gcccactttc
      301 ttttgtagta tgaaagaggg ctttacatat ttgtaaatat cgaagtacac ttgttttatc
      361 aataaattat ttaataaggt ataatggacc aattaaacaa taaaaattct cctaaaacag
      421 caagtattaa atatataata gcttcatttt tgtaaagaga ggtaattttt tcgcttatgt
      481 catcatcaat tgtaatagca atttcatctt gaataatatt tttatatata gatgggaaaa
      541 tatctttttt ctttataaga ttaaataaat tatttacacc tacccatatc aaatatccta
      601 aaacagcacc taatgtgttc atcattaaat catcaatatc tgtagctctt aagttaaaca
      661 attgacaaag ttcaattgct aatgaaaaac ctaatccagt taatgaaaca ttgataatat
      721 ttctaaaatt tttccaaatt aaaggtagta aaaatcctaa aggcataaac ataattatat
      781 ttaaaatata tgttaagatg ccaactgagt caaaaggtat tagatttatt tcatcaattc
      841 taaaaagccc actatacctt cctatatccc atatgctacc aattccagct ttactaagtg
      901 ctagatagat atataataaa aaaatatata cccatactaa atgtatcatt acattctttt
      961 caactttcaa attttttcgt tttataacga ctatttggta tattaagcat ggtaatataa
     1021 cgcaaataaa tgaataaaat gtccatatca ttttattttg tcccctttca aatttatgtt
     1081 tttagtttta taagtactat ctttatatta aactgtaagt tttaaaaatc ttttaataaa
     1141 atcttaaaaa tttattaatt tataaaaata tattattaag attaaaaa
//
LOCUS       DAFKMX010000354         1169 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.364, whole genome shotgun sequence.
ACCESSION   DAFKMX010000354 DAFKMX010000000
VERSION     DAFKMX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.364"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..>1169
                     /locus_tag="LZB08_003766"
     rRNA            <1..>1169
                     /locus_tag="LZB08_003766"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          299 a          264 c          357 g          249 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct ccttaatcgg agagttccct
      781 tcggggacat tggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
      841 gttaagtccc gcaacgagcg caacccttgt ctttagttgc catcattaag ttgggcactc
      901 tagagagact gccagggata acctggagga aggtggggat gacgtcaaat catcatgccc
      961 cttatgctta gggctacaca cgtgctacaa tgggtagtac agagggttgc caagccgtaa
     1021 ggtggagcta atcccttaaa gctactctca gttcggattg taggctgaaa ctcgcctaca
     1081 tgaagctgga gttactagta atcgcagatc agaatgctgc ggtgaatgcg ttcccgggtc
     1141 ttgtacacac cgcccgtcac accacggga
//
LOCUS       DAFKMX010000355         1143 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFKMX010000355 DAFKMX010000000
VERSION     DAFKMX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.365"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(80..1078)
                     /locus_tag="LZB08_003767"
     CDS             complement(80..1078)
                     /locus_tag="LZB08_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBM7317192.1"
                     /translation="MISLLMSLLTPFIIAFVLAYIFNPLVKFLESKLNFKRIYSLLLT
                     YGVLIVILISFILFTVPSIVNSLADLVAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKE
                     YGDKIMSVMPKFSNLLIGSLGGIFSTTFSVGKFIVQFLLGFIICFYILLEKEKFLLFT
                     KKVVYISLGKKYGDFIIELCQSLNLNIGKYFTGKILDSFIVGVLSGIGLYFLKSEYAL
                     LFGTLMGVMNMVPYFGPVIGMAPVVIINLFSNPTIALTSLIYLIIIQQVEVTFIEPKI
                     VGGQLGLSPFFTILAVTVGGGFFGIPGMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT          447 a          171 c          144 g          381 t
ORIGIN      
        1 aatattacca ttaaagagaa taatattaca gttacacagc tgtaactttt gattacaaaa
       61 tcgtaattaa attgtatttt tattccattt gtattttatc atgtctacga tttacaaact
      121 cacagaagta tatctttata acgcccatca caggagcaga tagaatcata ccaggtatac
      181 caaagaatcc tccaccaaca gtaactgcta ggattgtaaa gaatgggcta agacctagtt
      241 gtccacctac gatttttggc tctataaatg taacttcaac ttgttgaatt attattaagt
      301 aaattaaaga agttaaagca attgttggat tggagaataa attaattatt acaactggtg
      361 ccatacctat tactggacca aaatatggaa ccatattcat gacacccata agtgtaccaa
      421 ataatagtgc atattcagat tttaaaaagt aaagaccaat tccagaaagt actccaacta
      481 taaaagaatc taaaatctta cctgtaaagt atttaccaat atttaaattt aagctttggc
      541 atagttctat tataaagtct ccatattttt tacctaagga aatataaact actttttttg
      601 taaacagtaa gaatttttct ttttctaata atatatagaa acaaattata aaacctaaaa
      661 gaaattgtac tataaatttt ccaactgaaa atgtagtgct aaatatccca ccaagtgaac
      721 caataagcaa gttactaaat ttaggcatta cactcataat tttatcacca tactctttta
      781 atgtattagg gtcaactgat tgtaaactct tacctaagtc gaataaaaac tgctctgttt
      841 tatctatgta tgtaggaatt tgagcaacta aatcagctaa actatttact atggatggaa
      901 ctgtaaataa tataaaggaa attagtataa ctatgagtac accataagta agaagtaacg
      961 aatatattct cttaaaattt aatttacttt ctaagaactt tactagtggg ttgaatatat
     1021 atgctaatac aaatgctatt ataaatggtg ttagtagtga cattaataaa cttatcactc
     1081 caaaaaaata tttgtaatta tcaataactt taatcaagat atatgaaaca actacagtga
     1141 gta
//
LOCUS       DAFKMX010000356         1137 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.366, whole genome shotgun sequence.
ACCESSION   DAFKMX010000356 DAFKMX010000000
VERSION     DAFKMX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.366"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            47..>1137
                     /locus_tag="LZB08_003768"
     CDS             47..>1137
                     /locus_tag="LZB08_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBM7317193.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVI"
BASE COUNT          419 a          122 c          169 g          427 t
ORIGIN      
        1 gtgattttgt gataaaattt aattatatag aaataaggag gaaaaaatga aaattaattg
       61 gaatacaaaa tacacaacaa tagcaattta tacatttata atagctgcta gtagtattat
      121 attttatcta gtatcatcac aaattgatgt attttctaat aatttagatg ctatatttac
      181 aacattacaa ccttttataa taggatttgc tattgcatat cttcttaatt ttattttaaa
      241 gttttatgaa gacagaattt ttataaaaag tgagaaatta aaaaaactaa aacaaagttc
      301 aaaaagaggc ttgggtcttt tattaacata tgcaacagct acattgatac tttacttatt
      361 tatgcatttc gtattgccac aagtaattga gagtattgtt ggactagcaa atgatatacc
      421 tatgtatgta aacaatgcaa ctaagcttat tgataaatta atgactgatt taaatcttga
      481 tgagcaatac tttaatttgg cagttgataa gtggaatgag tttgttacat atattataaa
      541 gtttgtaaca gatttaatcc caatattagg aagtatgtta aagaatgtag cctctagtat
      601 atggaatatt gtactaggat taattgtttc agtatactta ttaattgata aagaaaaatt
      661 ctatggatta agcaaaaaaa taacttatgc agtatttaca gaaaaacaag ctgctagaat
      721 tttagaatta actcatagaa gtaactatac atttggaaga tttttaggtg gcaaaatatt
      781 agattcattc attataggta ttctaacatt tgtaatactt acattagtta aaatgccata
      841 tacattatta atatcagtta taattggtat tacaaatatt atccctttct ttggaccttt
      901 atttggagct ataccatcca cgctaatagt gatgtttgta tctcctataa aagctttctg
      961 gttattgtta attatattaa taatacaaca gatagatgga aatattatag gacctaaaat
     1021 acttggagat tcaattggta tatctgcatt ttggattttg ttctccttat tggttgcagg
     1081 aaaattatta ggttttattg gaatggttat aggagttcca atgtttgcag taattta
//
LOCUS       DAFKMX010000357         1125 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.367, whole genome shotgun sequence.
ACCESSION   DAFKMX010000357 DAFKMX010000000
VERSION     DAFKMX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.367"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..654)
                     /locus_tag="LZB08_003769"
     CDS             complement(<1..654)
                     /locus_tag="LZB08_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317194.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     "
BASE COUNT          452 a          175 c          141 g          357 t
ORIGIN      
        1 tgtaatacca attatgatag aggcaattcc aagaactaaa gttcctatac ccatatttac
       61 atctgagaat cccaagaact gagccataag actaccagat aaagctataa gtccatttga
      121 aatcataagt cctagtattt ttatagaacc tatatttata cctagagatt taatcatttg
      181 agagttatca ccaacacctt ttaaagtgta tccaagacct gtttttagga ataaatctaa
      241 aaggacttta cagatgaata caaaagctaa ggctaaaact ataggagata tttccccatt
      301 gaataagtgt ttaaagctaa atagaggaat atttgatttt cccattattc ttaaattgat
      361 agagtatagc attcccataa ctaaaatacc agaaagtaaa ttagatatct taaacttaat
      421 gtgaagaatc cctgtaacaa gaccagcagt acaaccacat aagatagcca ttaaagttgc
      481 tactacagga gaaataccat tagtaagaga aaatgctaca atagaagcac ccattgtata
      541 gctaccatca gcagacatat ctggaaaatc taagattttg taagttatat aaacccccag
      601 tgccataatc gataaaatta aactttgcgt cattacagat ataatacctg acataaataa
      661 aacacccctt tgaaaatatt ttaaatggtt ttttaaattt ggttttaaaa aatgattttg
      721 atataaaaaa accacacagt atatataact gtatggcaaa aattcgaata agccacacag
      781 tataatccat gtggctctat attatgatga taaaataaaa actttaacaa caattactat
      841 agccaacata gatacaatcc ttaaaaatca taatcctaag gggcttgtat ctatgttatg
      901 acaatttgtc ttaacttagg tacaaaccct aactaaattg gctactaaaa aatcgtactt
      961 gtttgtttaa taaagtcttc atatttacct ctccttttaa tttaataatt ttgaaaattt
     1021 tgttttctgt tttgtatagt aattagttta atacagtaaa aaataaaagt caactataaa
     1081 ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa taaaa
//
LOCUS       DAFKMX010000358         1122 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.368, whole genome shotgun sequence.
ACCESSION   DAFKMX010000358 DAFKMX010000000
VERSION     DAFKMX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.368"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(226..1095)
                     /locus_tag="LZB08_003770"
     CDS             complement(226..1095)
                     /locus_tag="LZB08_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBM7317195.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITSEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIEELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          423 a          176 c          106 g          417 t
ORIGIN      
        1 atcgtaaata ataatctaaa atacatcaaa aaataaacta aaaaatatcg aaaaataatt
       61 taaaagacat caaaactaaa tcttctaatc agcaattaaa agtctatttt cttagtttaa
      121 aaaataaaga tagatttttt caaaacacta gataaatagg taaaaaaata aacagttagt
      181 aaaataaatt tttactaact gtttattttt attgtttaat atgtcctata aatcctctaa
      241 gtaatatttt ccttgcaaaa attctgattc taaacctaga tttttaaaat aatcgaccat
      301 gcccatacac gcaaattttt ctgtagaata atgagtagca cctataacat taattaaatt
      361 atcttttgct atttgatgga actctaatac tggttcaaaa tgttttaaag gcttagtaaa
      421 cccagtaata tatagattta taccaagttc agcaacttct cttgctacaa atggataact
      481 tcctccccct gctgcaatag caactaaacc atcttttaat acagaatctc cataatcata
      541 caatttcaca tcatgaccaa cagatttttt aattaattcc tcaatctctt ctattgattt
      601 tagtgatgtt ttacatataa ctcctacctc aattttatcg tcatatttac aaaatggttt
      661 gactatttct aaacccatag cttttgcaaa actaacagaa gttgaatagt cactatagtt
      721 gtctagtgga gaatgtaata cataaaaaga aattctacga tttttcatct cttccatata
      781 agatagtggt atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc ccattgcatg
      841 atgtgaaaaa attaaaatat ctttttcatc tttatctata acctgcttaa ttatttcttt
      901 atcaggaaaa gtagttgtgt atactttatt aatattttga gcattatcta atactaatcc
      961 tatgtatttt tttctaaatt ctgaagtgat aaaatcattt tcaaaattca taaaactcca
     1021 atcatcatta atatttttaa tattaaattc taaatctaat tttttataaa gttcaattgc
     1081 cttaataata accactcctt tataataata ttgaattact ac
//
LOCUS       DAFKMX010000359         1103 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFKMX010000359 DAFKMX010000000
VERSION     DAFKMX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.369"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            113..253
                     /locus_tag="LZB08_003771"
     CDS             113..253
                     /locus_tag="LZB08_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317196.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(250..621)
                     /locus_tag="LZB08_003772"
     CDS             complement(250..621)
                     /locus_tag="LZB08_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317197.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          434 a          114 c          129 g          426 t
ORIGIN      
        1 taatcaaaat atattttata aaatcaaaat acaattacat aaactttatt aaaaatttgt
       61 tgggaatttc tgactaacat taagaacgta agtttgttgg gggatggtat atgtggaaaa
      121 gtcaaacact actgttacaa aaaaagaaaa aattgaagat ataggaatga aattagaaca
      181 tttattgagt gaagatatta ctttttatga attattactt gagattttgt ataaagaaaa
      241 agaagagaat taattctctt ctttaaggaa tctttttaaa taggcattat caatcatttt
      301 ttctatagct tctctatctt ctttttcaag agaatatatt ttttctaaga gcttttttgt
      361 tttttcactt actgataagt tctctatgat taatttatct tctttgctta tttttgtatt
      421 atcatttacg cctaataaat aatctgttga tgtttctaat gcttttgcta atctaactat
      481 tatttcactt ttaggttctc ttaaattatt ttcataacgt gacaagctag cctcagttat
      541 accgacttta gtagcaagtt ctttttgatt tatatctaat tcttttcgta gatttgctat
      601 tctatctcct aaactttcca tatgtacatc actccttacc gaaattataa gttattttga
      661 taaaaagtaa aataaaatta ccaaaaatat aaaaaaaaag ggttaaaata ataaaatata
      721 ttgaaaatta ccatttgtat atgtataata tgaattatac aaatggtaat ttgatgagag
      781 gtggtatgtt gaaataagaa actgaattaa ttaataaata tatagaaagt tttgaaaacc
      841 cttaattgag atataaacaa tatgtacaaa attttttgca agtaaaatta ccaaatgtat
      901 aaaataaatc agtagcatag ataattttat gctttaatac attagattat ctaatttagt
      961 tataatggtc aaaaatgcaa tctacaaata aaacaaagta ttatcaaaaa agtattcttt
     1021 aagagaattt tttattagag atgtaattga tataaataat cgtattaaaa aataaaatac
     1081 taagtttaaa aatttaaata atc
//
LOCUS       DAFKMX010000360         1050 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.370, whole genome shotgun sequence.
ACCESSION   DAFKMX010000360 DAFKMX010000000
VERSION     DAFKMX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.370"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(230..721)
                     /locus_tag="LZB08_003773"
     CDS             complement(230..721)
                     /locus_tag="LZB08_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBM7317198.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKELSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          402 a          158 c           88 g          402 t
ORIGIN      
        1 cttttaagat aaataaaaat atctgcaact atatattgta aaaatttaaa actaatttat
       61 atatgctaat ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta
      121 acttatatat tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt
      181 catatctaag atatgtgatg acactaatat tcacaatttt tagtaattat cacattataa
      241 aaaatattac agctagtcca tcatcagatg cttcttgtat aaacttttta agctcaatta
      301 gatttgccca tacatattca aaaaaatcat cttcactctc atcattcata acaggataaa
      361 tattattttc taacatctca ttaatattaa atctttttct aaatgcttgt tcttccaaac
      421 tgttcattgc ttttgatagt tcttttacat catttgctgt aatcaacatg acaggaaatt
      481 ctccatcaat ttccataagt atgtttccac ttagtactaa tttgctaaat atattatcat
      541 catctccacc aaaaggacaa cctgtcaaag taaagtgaat ggcatgccat gctttatcaa
      601 tattgatagt actattttcg ttggcatctt caaaaacaaa atcttgtaaa ttttcttctg
      661 cttgctgtaa tttaaaaaca ttctcttcac ttattttagt ataacatcct atcatcccca
      721 taattacatt cctcctaagt agcgtttttt taattgtaac ttataattaa acttagttac
      781 aataaaatat attgagaaaa gtattatttc atcttaaatt taaataccta tattatcaac
      841 taaaattctg tgtttatagc gtaagtatta ttattttttc ctaaaaatca aatgaatcag
      901 aagttctata ggcaatattg actataaata agaactatta cttttaaaaa aaaatttaag
      961 aaattatact gtagaatcat gctctactta ttagctttaa ataaaaaata aatcatgttc
     1021 tcaaattaaa taataaataa caataaattt
//
LOCUS       DAFKMX010000361         1045 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFKMX010000361 DAFKMX010000000
VERSION     DAFKMX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.371"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            108..860
                     /gene="cwlD"
                     /locus_tag="LZB08_003774"
     CDS             108..860
                     /gene="cwlD"
                     /locus_tag="LZB08_003774"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBM7317199.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          466 a          108 c          159 g          312 t
ORIGIN      
        1 gtgaattaaa tagggacaga aatataatat cattacataa taatatatta ataccaataa
       61 ataaagatat atcaatatta aaaaaattta aatgtttgta ctaatatatg gaatattata
      121 gtgtttttat aagtatacat atacagggtg atgttgtgag aaagtacata aaacatataa
      181 tttttagttt tgcaatgata tgtctagtag tagtatccat atttgaaatt aaaaatattt
      241 ctgaagatgt tatcaagtat atgccagtaa caaacaaaac tataatttta gatgctggtc
      301 atggaggcat tgatccaggt gcattaaata aggataagag tacatctgaa aaggatatta
      361 acctagcaat aacacttaag cttagagagc ttatagaatc aagtggtggc cttgtaatat
      421 taactcgaga agatgacagt agcctttata aagaagaaaa taataaaact acaagacaaa
      481 aatataatga gaatttgaaa aatagaaaag aaataatatc taattctaat gccaatatgt
      541 ttgtctctat acatcttaat gcatttgaac aatcaaaata ctatggagct caaacttttt
      601 atcctaaaga caaacaagac agtaaagaat tatcgaagtg tattcaagaa gaacttaaaa
      661 gagtagtaga taagacaaac aacagagaag tcaaaccaag ggatgatata tatcttttga
      721 aagaaaataa tattccatca gtactgatag aatgtggttt tttatcaaat gaaaaagagt
      781 gtaaactctt aactgatgaa acatatcaag aaaaaatagc atgggcaatc tacataggaa
      841 tacaaaaata tttaagttga aataactata aaaataggtc taaatatgta aatagcttgt
      901 attaaagcag ttaaaatgca ttaatatagg ctatttttat tttgacaaaa aaatatttaa
      961 aataaaagtt aaaaagttgt tgacttagaa taatatagat gatattatat atgagtgccc
     1021 aaaaggagca ccaaaataag acaaa
//
LOCUS       DAFKMX010000362         1038 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.372, whole genome shotgun sequence.
ACCESSION   DAFKMX010000362 DAFKMX010000000
VERSION     DAFKMX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.372"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(2..73)
                     /locus_tag="LZB08_003775"
     tRNA            complement(2..73)
                     /locus_tag="LZB08_003775"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asn,seq:gtt)
     gene            complement(80..153)
                     /locus_tag="LZB08_003776"
     tRNA            complement(80..153)
                     /locus_tag="LZB08_003776"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Cys,seq:gca)
     gene            complement(161..236)
                     /locus_tag="LZB08_003777"
     tRNA            complement(161..236)
                     /locus_tag="LZB08_003777"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Lys,seq:ttt)
     gene            complement(245..321)
                     /locus_tag="LZB08_003778"
     tRNA            complement(245..321)
                     /locus_tag="LZB08_003778"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:His,seq:gtg)
     gene            complement(329..405)
                     /locus_tag="LZB08_003779"
     tRNA            complement(329..405)
                     /locus_tag="LZB08_003779"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Pro,seq:tgg)
     gene            complement(429..505)
                     /locus_tag="LZB08_003780"
     tRNA            complement(429..505)
                     /locus_tag="LZB08_003780"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Ile,seq:cat)
     gene            complement(520..593)
                     /locus_tag="LZB08_003781"
     tRNA            complement(520..593)
                     /locus_tag="LZB08_003781"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Met,seq:cat)
     gene            complement(600..675)
                     /locus_tag="LZB08_003782"
     tRNA            complement(600..675)
                     /locus_tag="LZB08_003782"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Phe,seq:gaa)
     gene            complement(679..767)
                     /locus_tag="LZB08_003783"
     tRNA            complement(679..767)
                     /locus_tag="LZB08_003783"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:tga)
     gene            complement(856..931)
                     /locus_tag="LZB08_003784"
     tRNA            complement(856..931)
                     /locus_tag="LZB08_003784"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Gln,seq:ttg)
     gene            complement(939..1015)
                     /locus_tag="LZB08_003785"
     tRNA            complement(939..1015)
                     /locus_tag="LZB08_003785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tct)
BASE COUNT          279 a          278 c          233 g          248 t
ORIGIN      
        1 gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg ctccaccgtt
       61 gagctacctt ggaatatttt ggaggcgaca cccagattcg aactggggat caaggagttg
      121 cagtccactg ccttaccact tggctatgtc gccacctttt tggtgatcta tccgcgactc
      181 gaacgcggga caccctgatt aaaagtcagg tgctctaccg actgagctaa tagaccatat
      241 gaattggggt ggataatgag agtcgaactc acgacctcca gagccacaat ctggcgctct
      301 aaccaactga gctataccca ccttatattg gtcgaggtga agggactcga acccccggcc
      361 ccatggtccc aaaccatgtg cgctaccaaa ctgcgctaca cctcgaattt atattaatta
      421 atttaatgtg gtgggccatc agggactcga accccagacc taccggttat gagccgggcg
      481 ctctaaccaa ctgagctaat ggcccacatt aaattttagt agcggtaata ggagtcgaac
      541 ctatgacctt tcgggtatga accgaacgct ctagccaact aagctatacc gccatatttt
      601 ggtgcccaga ggcggaatcg aaccaccgac acggggattt tcagtcccct gctctaccga
      661 ctgagctatc tgggcatttg gcggagaggg tgggattcga acccacggcc cctttcgaag
      721 tcactggttt tcaagaccag ctccttaaac cactcggaca cctctccaca ttcggatact
      781 taaacttatt ctaaaattga acctaactat atttaaaata aaaataattc aaatatagct
      841 tagtaataca tcatttggct ggggatgcag gactcgaacc tacgcataca tgagtcaaag
      901 tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac agggattcga
      961 accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg cacccacaat
     1021 ttatatatgt atatcaaa
//
LOCUS       DAFKMX010000363          996 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.373, whole genome shotgun sequence.
ACCESSION   DAFKMX010000363 DAFKMX010000000
VERSION     DAFKMX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.373"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            28..912
                     /locus_tag="LZB08_003786"
     CDS             28..912
                     /locus_tag="LZB08_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBM7317200.1"
                     /translation="MYAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLG
                     DAIVKVNNAEVDGYSDFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNL
                     LSGFATLTYINPDTSEFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCI
                     NAKRENIIGSFTENTDFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKC
                     KKYNIEIIDIENQKHPESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSH
                     AIENDPAVGYGVFIKWML"
BASE COUNT          415 a          113 c          150 g          318 t
ORIGIN      
        1 ttattatatt ttttctcaaa taatttaatt tatgcacaaa atttacaaaa aaatgaacct
       61 gaatatttaa taccaattgg aaacgtactt caaattgacg cagaattaaa aaatatcata
      121 gttagaaatc ctggagaagc atctccattt agattaggtg atgctatagt aaaggtaaat
      181 aatgctgaag ttgatggata tagtgacttt gccaatacct taaatgcttt accagaagaa
      241 aaacaagtat ctattttatt aaatagaggt ggacaactta taacaatgaa aactaccaaa
      301 cacatattag aaaaaattag ttttaacaat ttactttctg gatttgctac tttaacgtat
      361 atcaatccag atacatcaga gtttggagct gtaggtcatc caataagtgt tggtaatgca
      421 agaaaaatac ctattaaaac aggttcaatt tctacaacta agcaccttaa tatagaaaaa
      481 tcttataaag gtagtgttgg atgtataaat gcaaaacgag aaaatattat tggtagcttt
      541 acagaaaata ctgattttgg aataagaggt caaattaata actttgatat ttccaagtta
      601 aaaaaatata aagtagcatc tttagatgaa gttaaattag gaaaagctca aataatttta
      661 caagataact ttaataagtg taaaaaatat aatattgaaa ttatagatat agaaaaccaa
      721 aaacatccag aaagtaaaag ttttaaaatt aaaattacag ataaaaagct tttagctcaa
      781 actggtggta ttgtacaagg tatgagtggt actccaatag ttcaagataa taaaataatc
      841 ggagcagtgt cacatgcaat tgaaaacgac ccagctgtag gttatggtgt atttattaaa
      901 tggatgttgt aaataatata aataaataag ttttaagatg ttattaagtt attaataata
      961 tatttggtct aaaggtactt ttatttaacc tacaga
//
LOCUS       DAFKMX010000364          995 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.374, whole genome shotgun sequence.
ACCESSION   DAFKMX010000364 DAFKMX010000000
VERSION     DAFKMX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.374"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(146..799)
                     /locus_tag="LZB08_003787"
     CDS             complement(146..799)
                     /locus_tag="LZB08_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBM7317201.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
BASE COUNT          319 a          155 c          104 g          417 t
ORIGIN      
        1 ttgatttata tatgttaatt taaatacatt attctatcgt agtgccttag agtttttatt
       61 tgtatttaaa gttaaattta ttcttgtttt tcctttaata tttaatagta aaacaacaaa
      121 attataaaaa aaataaagag aaactttata aaagcttctc ttttttcatc tctgctatag
      181 gtatcgtttt ccaatgttct ctcaaaactc tttcaacatt ttctaaatca ttctccctca
      241 tagattttac tacttcaata tgttgattat tgctgtatgt caacatttta tataactcat
      301 cttcattatc actagtatat gactttgaca taaaattata tcttaaagag ttcaatagat
      361 ttactaacac ctgattatct gatttagtta tgtatatatc gtgaaattca ttacttaatt
      421 ttaaatagct attgtaattt ttatacttta tagagaccgc catcatttct gttaattctt
      481 ccatttttac taggtctgat tcagttaaat tttgtaatgc tgaagaaccg gctaacgcat
      541 ccaatactcc aattacggaa taggtgtcta atttctcctt ttcctgaaat tcttttacta
      601 caaatccctt attaggtatt ttttctagta aattttcacc tgctaaccta gttaatgctt
      661 ctctcgctgg tgttctacta actccgatag atttacaaat ctcagattca gtaattctct
      721 gattagcttc atattctcca ttttggattt tctttgatat atagtcatat acttgagttg
      781 taagtggatc taccttcata agacgttcct ccatattaag attataaaag ttaaatctcc
      841 ctacaacttt ggtagagagt tcagttttat cttactaatt tatataatat cacaattatg
      901 atatttttta tacttctttg taatattttg aaatactaaa attcttatga taatttatac
      961 aaaaaaagta gaaaaagttt tgataaaaca aaact
//
LOCUS       DAFKMX010000365          992 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.375, whole genome shotgun sequence.
ACCESSION   DAFKMX010000365 DAFKMX010000000
VERSION     DAFKMX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.375"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            225..629
                     /locus_tag="LZB08_003788"
     CDS             225..629
                     /locus_tag="LZB08_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBM7317202.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            646..>992
                     /locus_tag="LZB08_003789"
     CDS             646..>992
                     /locus_tag="LZB08_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317203.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYN"
BASE COUNT          423 a           83 c          142 g          344 t
ORIGIN      
        1 taaatagatt taaatttttt taaaaaattt ataaatattg ttcaaaaatc ctgccaataa
       61 attggaaata ctaaattaaa aaaataataa agtttagctt atagctaaat aaaagtttac
      121 ttataaggtg aactttttgc attttcagtt ttgacataca tatttttttg taatatattt
      181 atattatcaa ttagagataa ggagagtgag aagagatatt taatttggat aagacattaa
      241 aaaaggattt aaatcaggaa tatacagaga gcaaaagttt aattatgaat aaaagtaagt
      301 atatgaatgt ttgttttaag aattatatta aagtagtaga aggaatgtat actaagaggg
      361 atttaattcc attaaaatta ataaactcta tggataaaaa taataagatt agaaaaacat
      421 tatatgaatt atcttgtgaa tttgaatatc caaaaacttc tttatcttca ttgtttacgg
      481 agctgaaaaa aatggatttt ttacaaagag tgaaaaatgg tgagtatatg ataaatccac
      541 atatagcata taaaggtagt agagctgata aagaaagttt attagcagaa tataatcaga
      601 taaaaagacc taataaggtt agtaaataag gaagaaggtt tttatatgag tttaaaactt
      661 gatagaaatg ttctacagtg gtttgactat gtatttgaga atgaaaaaac tagtttgaga
      721 cactataatt ttaattgtac actaaaggag attagttcta ccagtttgaa caaagtagct
      781 tttattttag aaaaaaataa ctctaagtac tggaagcttt attttgaaat accagctgaa
      841 gttactttaa aacttaagca aaacattcat cctttattta gggaatatat atacgaacaa
      901 atatcactgt ataacaataa tcaaatttac aattttgtaa attcaaacat attaaaagtg
      961 tttaataata ttgctattta tcaatataat at
//
LOCUS       DAFKMX010000366          992 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.376, whole genome shotgun sequence.
ACCESSION   DAFKMX010000366 DAFKMX010000000
VERSION     DAFKMX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.376"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            206..961
                     /locus_tag="LZB08_003790"
     CDS             206..961
                     /locus_tag="LZB08_003790"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBM7317204.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
BASE COUNT          396 a          108 c          160 g          328 t
ORIGIN      
        1 tttttggtat aaagtcaaat atatttttat catttataca atgaaaaaga aatttatcta
       61 tattttgaaa aatccactta aattttttaa tgtaagataa attgtaagct tattatcact
      121 aatattattt tatataaatt atattagtga taaaataaac ttatagaaat ataacaaatt
      181 tttattaatt tcataggagg aatttatgtt gaaagttata attagtggct ataatggaac
      241 aatgggaaga gttttagcag aatgtgtaaa aaaagataat gaattagagt tggtttgtgg
      301 tgtagctagg tctgctgatg aaaaaggtca tcatagtgat acaaagcttt acaaaacaat
      361 gactgatgta actgaaaaaa ctgatgtaat tatagacttt tcacatcatg agactttaga
      421 accaatattg tcatatgctc taaaaaataa tacacctgta gtattagcta ctactggata
      481 tgacgaaaat gacttaaata aaatagaaga agcatcaaaa aacattccta tattattatc
      541 atcaaataca tctcttggag taaatatatt aataaaatta gttaaggatg cttcaaagat
      601 gttagaaggt tttgatattg aaatcataga aaaacatcac aatagaaaag aagatgcccc
      661 tagtggaact gctactatga tagctaatgc aattagagaa gtcttgcctg aaagtgaaaa
      721 tacacatggt agatatggta gagattgtaa aagaaaacca aatgaaattg gaatacactc
      781 tataagaggt ggcaatataa tcagcgacca tgatgttata tttgcaggtg ataatgaagt
      841 tcttacatta agccatcatt ctcaatctga tgaagtattt gcaaacggtt ctatagttgc
      901 agctaaattt ttaattggta agcaatctgg gctttataaa atgtcagata tattaggata
      961 agttttgtac ataataaatc tcaatctatt ta
//
LOCUS       DAFKMX010000367          981 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.377, whole genome shotgun sequence.
ACCESSION   DAFKMX010000367 DAFKMX010000000
VERSION     DAFKMX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.377"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            118..459
                     /locus_tag="LZB08_003791"
     CDS             118..459
                     /locus_tag="LZB08_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBM7317205.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            610..>981
                     /locus_tag="LZB08_003792"
     CDS             610..>981
                     /locus_tag="LZB08_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBM7317206.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYV"
BASE COUNT          431 a           67 c          157 g          326 t
ORIGIN      
        1 atattaataa taattattta aatatcttaa gtaaatcagt cttacatata tttccgatag
       61 attatattga taaaaaacac taaaattaaa ttaataaaag tacttggagg gatggaaatg
      121 aaagaggaaa attttattaa tgttgaatac gaggataaac gtttttcaat taatagtaaa
      181 gaagttttgt acatagaaaa gcaagagtat gaagtaaaga tttatactga aagtggtaat
      241 tatgaagttg gagaaaatgc tccttgtata agaaaactat tgaaagaaga aaattttttc
      301 aaatgcagta atagttatat tgtaaatcta gacaagtgtg aattttatct cagcaataat
      361 tgtatttttg tgaataataa ttttataaga attttaggtt cagaaagaaa aaagaaatta
      421 aaaaagaaat tagaaaaatt atatgattat gaagattaat attgagaggc tgtattttat
      481 acagcctctt tatttattga ttgtattatt aataaatttt aaattatgat aaaatataaa
      541 acaagtaact tatttcagaa taaaatactt tttaaaagaa agggacgaga tatgggagat
      601 tataataaaa tgattgaatt aaagaataac ttaggtatca gtgaaagaaa gttaaaatat
      661 ccttgtatct ataaacattt taaagggaaa tattatgcta caatgggtct tagcaaggct
      721 attgatgata tagaaaacat atgtgaaata tatggtaaag aaaatttaat ccaaaacaga
      781 aataaatata aattagttat tagacatact gaaagagaag aagatatcta tgtttatagg
      841 gatttagatg gaaactttta tcataaaaaa gaagaagata ctaatgattt agttttatac
      901 aagaccttat atgatgatac aggaatattt gcaagacctt tggatatgtt tttagaaaaa
      961 gtagatacag ataagtatgt c
//
LOCUS       DAFKMX010000368          978 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.378, whole genome shotgun sequence.
ACCESSION   DAFKMX010000368 DAFKMX010000000
VERSION     DAFKMX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.378"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(61..792)
                     /locus_tag="LZB08_003793"
     CDS             complement(61..792)
                     /locus_tag="LZB08_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317207.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVENLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT          358 a          146 c           81 g          393 t
ORIGIN      
        1 ataaaagaga agaaaaaact gtactttaaa aataaatcgg aagaaaataa attaatcatc
       61 ttatatcctc ctatttttca tgaactcaag cataactttt gaaaatattt gttgacgtag
      121 tctagaaaca ggtattctat ctccactttc cataactgca ttaccatttt catatccata
      181 tacatattgt aaattgatta tataactacg atgacaccta aaaaaatttg ttggtaaact
      241 tctctctaaa ttttcaatag tctcataata atctacagtc tctttcttta aatgaacata
      301 aatctttctt tttataactt cacaatataa aatatcagca agttttaatt tccaatatga
      361 ataacctttt tgtattgtga tataattact ttcattatca tcaagttttg taactatttt
      421 atccaaagtt ttatatagtt tctcattaga tagaggtttt atcagataat ttatagcatc
      481 tacttcaaaa gcatcaaata caaattcatt taaaactgta acgaaaatta taaagcactc
      541 attaccatat gaccttaatt ttctagctgc ctgcattccg ttaatctctt ccatctgtat
      601 atctaaaaat acaatattaa acttttcatg attgcatagt aaattttcta ctttattaaa
      661 aagctctata ttatataata aattcttctg cttcatgtat aattctatat tttcctttaa
      721 atgatttagc atataggatt catcatcaca aatggcaatt gaaatcaata tatacacctt
      781 ctttcttatc attattctaa ttattatata aagttaattt tttctgttca attaatatga
      841 tctaatatgt tgaatatttg ttccaaaatg taataatctc gcttttcata gtatatctaa
      901 tttgaatttg tgtatcaata ttataataaa ctaatttatc acttttataa atttaattta
      961 aaaaaatgca aatttcga
//
LOCUS       DAFKMX010000369          970 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.379, whole genome shotgun sequence.
ACCESSION   DAFKMX010000369 DAFKMX010000000
VERSION     DAFKMX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.379"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(158..868)
                     /locus_tag="LZB08_003794"
     CDS             complement(158..868)
                     /locus_tag="LZB08_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317208.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTLDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          336 a          134 c          113 g          387 t
ORIGIN      
        1 aactctaaaa actaagcttc aaaaaattaa attttgaaag attgaaattc tagaaattaa
       61 tcttcaaaaa attaaatttg aaaaaacaaa actctaaaaa ctaagcttta aaaaattaag
      121 tttcaaaaat aaaattctag agattgaact tcaaaaatta agattctaac atataccttg
      181 taaattttcc tacaacctct tgatattttt tacggcttat aggaattttt tcattctgaa
      241 ctttggcata agtttgagtt atactatcta catggtttag attgacaata tacctttgat
      301 gcgtacgtat aaaattagag ggaagaacag tttccatatc atttaatttt ccgtagtatt
      361 ctaatgtttt atcaagggta actacagtta attttcttag ctcactttta aagtagagga
      421 tatcttttgt attaatttta caaagattag ttccaatctg aactgtaata aagttttctt
      481 tccattgttc taattttttt agtgcttctt ttaatacttt acatattttt tcagttttat
      541 aaggttttaa gatgtagttc aaagctccta catcatatcc atgaaagaca tagtcagaaa
      601 aacctgtgac aaagatgatt atagtatctt ttgaatgttt tcgtatagtt tttgctatgt
      661 caacgccatt actttttcca agttcaatat ctaaaaaaat aatatctatg taataatttt
      721 cgtgttctag attgtatact agactttctc catttgaata ttcttttatt tggtaggggt
      781 agccccacaa atccatttct cttgtaatga ttttattcag catgtttctt tgttgttttt
      841 catcatcaca aatacaaatc gaagtcaatt ttttatctcc tttcaagttt tatatttata
      901 tagttaagta tataatatat tttgctacct agatatataa aatgaaataa caattatatc
      961 cagatagcaa
//
LOCUS       DAFKMX010000370          969 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.380, whole genome shotgun sequence.
ACCESSION   DAFKMX010000370 DAFKMX010000000
VERSION     DAFKMX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.380"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            59..748
                     /locus_tag="LZB08_003795"
                     /pseudo
     CDS             59..748
                     /locus_tag="LZB08_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
BASE COUNT          303 a           87 c          229 g          350 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagat
       61 gaatgagttt aaagatatgc ttttagggat tgatttaaaa gctgagttag taggtgtact
      121 tcatactatt aatgatggtt tagcagatgc agtccaatgt gatgagttaa gagtattgta
      181 tggaagagat tatatacaag aagaaatttt aggtcttaag tttaagattt caccattttc
      241 gtttttccaa accaatacta agggagcaga ggttctttat agtattgcta gggattttat
      301 tggagattac aatgataaag ttgtatttga cttgtatagt ggtactgggt ctattggaca
      361 ggttatggct ggtgctgcta agaaagtgta tggtattgag atagttgagg aagctgttgt
      421 tgctgctaat gagaatgcta agttgaatgg acttactaat tgtgagttta ttgctgggga
      481 tgtggctaag gttgttaagg atttaaagga caagcctgat ttgattattg ttgaccctcc
      541 tagaccaggc attcataagg atgctattag ggatatttgt ggatttggtg ctaaggagat
      601 tgtttatatt tcttgtaatc ctaagtcttt ggttgttgat ttggttgatt ttaaggggta
      661 tggttatgag attaagatgg ttaagtgtat ggatatgttc ccaaatacgc cacattgtga
      721 gactgtagtg ttgctacgtc gaaaataatg ctagaaatct tgagctttag acagtttcat
      781 agacatataa tatttagatt agagggtaat aaaattagga ataaagaatt gaaaaggtgt
      841 tttgatgtgt ctgtagttat atatatggag tgtgggaata cgaaataaaa aaagttttaa
      901 ttatagaaat agatattaaa tttataaatt taatatctat tttttatgtg aaaatatatt
      961 taatctaaa
//
LOCUS       DAFKMX010000371          933 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.381, whole genome shotgun sequence.
ACCESSION   DAFKMX010000371 DAFKMX010000000
VERSION     DAFKMX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.381"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            24..896
                     /locus_tag="LZB08_003796"
     CDS             24..896
                     /locus_tag="LZB08_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBM7317209.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
BASE COUNT          371 a          110 c          135 g          317 t
ORIGIN      
        1 aaatataata aaggagattt taaatgaata tattacaaaa tgaaaattta attatcgaat
       61 caagcagttt tggtgctgaa ctaactaaaa tattttctaa aaaatacgac aaaaacattc
      121 tttgggatgg taataaaaaa tattggggta gacaatcacc tatattgttc cctatagtag
      181 gtaaactttt tgataatgaa acaataatag aagaaaattt atatagcatg actcaacatg
      241 ggtttgctag agatatggac tttgagattg tagataaagg agatacattt gtaacttata
      301 aattaacaga taatgaatct actttaaaga agtatccata ttcttttgag ctaatcataa
      361 actatacatt aaatgataat agtattgagg tagaatggat tgtaaaaaat actgattcta
      421 aagatatatt cttttctata ggtggacatc ctgcattcaa ccttcctttt tacgaacaaa
      481 atgatttttc taaacactac cttgagttta aaagtaaaaa tgatgtagaa aaaatcaatt
      541 taaatggttc ttttacagat gatataatac ctattggaaa attaaaaaac ttacagttaa
      601 atccagaagt attcaagaat gatgctttga tatacaccaa catagatgaa gtgtctatat
      661 gtaataatga tggtagtaaa tatgtaacta tatctatgga agatttccct ctagttggta
      721 tttggactcc ttattatagt gaaactaatt ctactgctcc ttttttatgt atagaacctt
      781 ggtatggtct tgctgatagt ataaattcta acaaaattta taaagataag aagtttatta
      841 ataaacttag taaaggaaaa gtttttactg cttcttacaa tataaatata cactagataa
      901 aggttagcta acccgttccc aaaataataa aat
//
LOCUS       DAFKMX010000372          922 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.382, whole genome shotgun sequence.
ACCESSION   DAFKMX010000372 DAFKMX010000000
VERSION     DAFKMX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.382"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            143..571
                     /locus_tag="LZB08_003797"
     CDS             143..571
                     /locus_tag="LZB08_003797"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBM7317210.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT          433 a           79 c          119 g          291 t
ORIGIN      
        1 ataaagcagt atataaaatt gtagaatcaa caatagcata tactaaaaca tccaccttat
       61 attaactaaa tcatatctaa ataaaataca cttacctaca aacattataa aatcaataca
      121 aaaatgaggt gaaacaaaat ttatgataaa gaaattaaac aataaagata taaataaaat
      181 catggaaata tgggaaaaaa gtacaatcaa agcacatgac tttataagta aagaatactg
      241 gcaaaataac tataatactg ttaaaaacga atatatacct atatcagata catttgtata
      301 tgatgatgga gatgaaataa aaggatttat aagcataata gataaaagct ttataggagc
      361 tttatttata aaacccaaat accaaaatct aggtatcgga ggtaaacttt tagattatgc
      421 aactaaaaaa tataaaagtc taagcttagc agtatataaa gataataaaa aagcagttgt
      481 gttttataat aaaaaaggtt ttaatatagt aaaagaacaa gtaaatgaag attcaggatt
      541 taaagagtac ataatggaat atagtaaata atatgattac attctattaa aaggataatt
      601 aatttatttt ctagttgatt tttataagag taatttttaa aatagactat atgaagataa
      661 agatatttat tatagatgta agttaatcag aattgatata gctacaaaag aggaaaataa
      721 aattgtatgg tgtatttcag atttaacaaa tttactattt aaagataata agtttgatat
      781 aatattaaag atactttcac ctataaacta tagggtattt gaaagaattt taaataatta
      841 tggaatgcta ataaaggttt gttaaaattt aataattatt agattataga aaattgccat
      901 gaaattagtt catcaagaac ta
//
LOCUS       DAFKMX010000373          916 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.383, whole genome shotgun sequence.
ACCESSION   DAFKMX010000373 DAFKMX010000000
VERSION     DAFKMX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.383"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            31..>916
                     /locus_tag="LZB08_003798"
     CDS             31..>916
                     /locus_tag="LZB08_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBM7317211.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEIAKLDLFDNYDFLSLNTFELREGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFIKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYP"
BASE COUNT          375 a           82 c          146 g          313 t
ORIGIN      
        1 gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa tacaagagag
       61 actattgaag attttagaga taaattttat gtagcagaaa attcttatct tatattaagt
      121 gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat aacttttaat
      181 gattgtctta aatttgatga aatagccaaa cttgatttat ttgataatta cgatttttta
      241 agcctaaata catttgaact aagagaagga gaagctgtta tagaagaggt aaatatgtat
      301 ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt tgaatttata
      361 aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa tctatttaag
      421 ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt ggaaaaagtt
      481 gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa gaatcatgta
      541 tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac tagacctctt
      601 ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata ttcaaatgta
      661 aaaaaatata atttagaaaa ttttcaaggc atagattttg gaattgacaa attatacaat
      721 ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc ttccagagta
      781 ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat atcagcacca
      841 cttacaatta taactggtat atatggaatg aactttagat atatgccaga attaaactgg
      901 atttatggat atccag
//
LOCUS       DAFKMX010000374          897 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.384, whole genome shotgun sequence.
ACCESSION   DAFKMX010000374 DAFKMX010000000
VERSION     DAFKMX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.384"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            32..>897
                     /locus_tag="LZB08_003799"
     CDS             32..>897
                     /locus_tag="LZB08_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBM7317212.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYFSQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARFQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDV"
BASE COUNT          401 a           95 c          129 g          272 t
ORIGIN      
        1 aaaaagtgta tcaaaaaaca attaaagtga ggtgatagca gtgaaaaaag cttataagtt
       61 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      121 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaacg ataaagaaac
      181 ttttacttat aaacagtgta gttctgattt gaccaattta aaaaaagaat taaaatggct
      241 taaagaacct gacaaatttt cactacaaaa cgccttaaaa gatttagata atgcatataa
      301 aaaattcttt aaagaaaaag caggatttcc taaatttaaa tcaaagaaaa ttaatagatt
      361 ttcatataaa actaacttta caaatggaaa cattatgtac tttagtcaac atataaaact
      421 acctaaactt ggtatggtta agataaggga taaacaagta cctcaaggta gaatacttaa
      481 tgctactata tctaaagaac caagtggtag atattatgta tcattatgct gtactgatgt
      541 agatattgaa gcatttgaaa atactaataa tcagataggt ttagatttag gaattaaaga
      601 attttgtatc tcaagttgtg gagaatttat agaaaatcct aagtatctta agaagtcatt
      661 aaacaaactt gctaaattac aaagagaatt atcgagaaaa acaatcggta gtttaaatag
      721 aaataaagca agattaaaag ttgcaagatt tcaagaacat atagctaatc aaagaaatga
      781 ttttctacaa aaactatcta ctaaattaat aaaagagaat gatattattt gcatagaaga
      841 tttacaagta aagaatatga ttagaaatcg taaactatct cgcttaattt cagatgt
//
LOCUS       DAFKMX010000375          837 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.385, whole genome shotgun sequence.
ACCESSION   DAFKMX010000375 DAFKMX010000000
VERSION     DAFKMX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.385"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(168..>837)
                     /locus_tag="LZB08_003800"
     CDS             complement(168..>837)
                     /locus_tag="LZB08_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317213.1"
                     /translation="ILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNVDIIFMDID
                     LNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYSLFKEAIS
                     SYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFKSSMKEIE
                     SNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLREKLNSCLV
                     DVII"
BASE COUNT          303 a          103 c           63 g          368 t
ORIGIN      
        1 ctttaaaata aaattgttaa tagaattgta aaataaaatc ttaatatatt caatctgtta
       61 ttgatgaaag gtatattttt aactataatt agtttttatt ataaattttt tcatatttta
      121 caatagatat attctattag aatagaatat aagggcaaaa atttacctca aattattaca
      181 tctaccaaac aactatttaa cttttctctc aataatttta tcttataacg acttacaatt
      241 attgaatttt gatttataaa tacaatatta ttttctatat ctagcatatt taccttcatt
      301 aaatttataa ggtagctttt atgacatcta aaaaaattat gttcttctag atttgattct
      361 atttctttca tactactctt aaaagtatat attccttttt cagtatgtat agttaaatca
      421 ttttttaaaa cttctaaata taaaatatca tctaccttta tgaaatatat cttgcattta
      481 cattttatag ataaaatagc ttcatttttg tagcattctt ttaaatatga tgatattgct
      541 tctttaaata gagagtattt taagggcttc tctaaatatc tataaactcc tatctcataa
      601 cctttcttta tgtatttatt tgatgaggat acaaatataa ttcttacact ttcatcaaat
      661 tttttgattt cttttgaaac attaaatcca ttttcatcat ttaagtctat atccataaat
      721 atgatatcaa cattattagg atactttttt aataaatcgc ctccattttc aaactctttg
      781 atattgtatt caaaattgta attataaaaa aaggtattta tataatcatt tagaatt
//
LOCUS       DAFKMX010000376          799 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.386, whole genome shotgun sequence.
ACCESSION   DAFKMX010000376 DAFKMX010000000
VERSION     DAFKMX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.386"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            40..606
                     /locus_tag="LZB08_003801"
     CDS             40..606
                     /locus_tag="LZB08_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBM7317214.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          311 a           68 c          146 g          274 t
ORIGIN      
        1 ataataataa aaaaattttt aaagagggtg atattacata tgagaaaact tgaagagata
       61 ttagaatata ataaatcatt tgtagagaac aaagaatatg aacaatatgt gacatcaaaa
      121 catccaaata aaaaaatagt agtcttatct tgtatggata caagattaac tgagcttttg
      181 cctaaggcta tgaatttgaa aaatggagat gtgaaactaa taaaaaatgc aggagctact
      241 ataatgcatc cttttggaag tattatgaga agtgttttag ttgcaattta cgaatttgat
      301 gtagatgagg ttatggttgt agggcatcat ggatgtggta tgtgtaatgt tgatacggat
      361 aagttacttg gtaaaatttt agatagagga atatctaatg atgtaatact tacacttcgt
      421 agtgctggaa ttgatacaaa acaatggtta catggattcg attctgcaga agaatctgta
      481 aaagatagta ttgatttaat aaaagggcat cctttaattc cagatggtat tattgtacat
      541 ggtctaatta tatcacctga aactgggaag ttagatgttg ttgtaaatgg atatgaagat
      601 aagtagagta tcataatgtt atataatcaa gatactgttg aatatgatgt tgtttcaaaa
      661 tttattttgg gacaactttt ttatttgaaa atatatgaaa aggtttattt taagctttgc
      721 aataaaaaat ataagtagct gactaattaa aaatcttact attatttatt aaaattataa
      781 ctatatgata taaaaatat
//
LOCUS       DAFKMX010000377          777 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.387, whole genome shotgun sequence.
ACCESSION   DAFKMX010000377 DAFKMX010000000
VERSION     DAFKMX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.387"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            38..649
                     /locus_tag="LZB08_003802"
     CDS             38..649
                     /locus_tag="LZB08_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBM7317215.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
BASE COUNT          320 a           91 c           94 g          272 t
ORIGIN      
        1 tatataattt ttaataatat tattttagga ggtaaaaatg ataaacgata ttaaaaataa
       61 attcaaaaat tacaaaccat atataaatgg atacaagaat atgaagagag catctgtttt
      121 aattccaata gtagaaataa ataacacaca ctatatatta tttgaagttc gctcaaaaaa
      181 tttgaagcat caacctagcg aaatttcttt tccaggtggt aaaatagaat caggtgagac
      241 tccatatgaa gctgttatta gagaaacttg tgaggaactt ggaactttta gtgacaatat
      301 agaagtaata tcaagtcttg acctccttat aacaccagta aagtttatca tacatccata
      361 cttaggatat ataaaaaata ttaataattt aaatataaac aaggatgaag ttgaccatac
      421 ttttttggtt cctataaaat atcttttaga gaatcctcca aatacttata gtaatactgt
      481 aaaagttttt ccagatgaaa atctaccata tggttcaatt ccaaaccaaa aagattataa
      541 atttgaaagt ggaagttatc ctattctgtt ttataaatat aaagattatg tgatttgggg
      601 aatgacagca aaaatactag aaaacttttt aaatttttta tcagaataac actcataata
      661 aattatcaaa acttttacat aagaaattta taaaagtttc taaatttaac aattctaaaa
      721 tattaataac tatattttgt agcacttttt tattttatta ttgtttgaat atttgat
//
LOCUS       DAFKMX010000378          775 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.388, whole genome shotgun sequence.
ACCESSION   DAFKMX010000378 DAFKMX010000000
VERSION     DAFKMX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.388"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            179..691
                     /locus_tag="LZB08_003803"
     CDS             179..691
                     /locus_tag="LZB08_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317216.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
BASE COUNT          339 a           62 c          123 g          251 t
ORIGIN      
        1 tctaactaat aacaatttaa aataagtaac ttatgtaaga atcgtaggaa ttacagagat
       61 atcctgtaga gaattaataa gacatattta agtatgcaaa attctattaa gtagaaacct
      121 tgtaacttta gtcatgtgag attcataagg atatttaagt attgaaagga gagtttaatt
      181 gaaaaaaata tctcaagaaa aaataagtac acatatccaa gaaataccac aggaaaaaat
      241 tgaacagtgg aaatctttat atgaaaaata taaaagtatt ttaaaagtaa attctaagtc
      301 tgttttagaa ataatagagt ttttaaagcg aaaatatgta atagtagaag aaacttcaga
      361 ggaatttaaa catgtagtta tcaataatat taagttaaac aagccttttt cacaaaagat
      421 ttccagtgat aaagaattaa aacctattgt atttacaatt ccaaatgaag gaaatgcaaa
      481 aaaattgtac gatactcaag aggaaattta tagtggctgt aagattattg taggtatgga
      541 atttgaaaca ggttgtgttt ttgtagaagg tagtgatgag cttaatgatg aaataacttc
      601 ttttaaagga ttagataaat atgatataaa taattattat ttagtagcaa actatattag
      661 atgtctaaag aaatataaat taatagatta gtgtttgatt taaagattat agagtaaatt
      721 tgaagttagt gtgataatga aactaagttt aaatctaata taactgtaaa acata
//
LOCUS       DAFKMX010000379          753 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.389, whole genome shotgun sequence.
ACCESSION   DAFKMX010000379 DAFKMX010000000
VERSION     DAFKMX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.389"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..>753
                     /locus_tag="LZB08_003804"
     rRNA            <1..>753
                     /locus_tag="LZB08_003804"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAFKMX010000380          744 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.390, whole genome shotgun sequence.
ACCESSION   DAFKMX010000380 DAFKMX010000000
VERSION     DAFKMX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.390"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            181..651
                     /locus_tag="LZB08_003805"
     CDS             181..651
                     /locus_tag="LZB08_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBM7317217.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKEVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT          334 a           58 c          135 g          217 t
ORIGIN      
        1 gaagatattg aaaaaataaa agattgatat aagatactga agaggtagaa acaaaagtgt
       61 taatagaaga tactaaaaaa taaaaaataa aggattaata aaaggtactg aagagataga
      121 gaaaaaaatg ttaatagaag atactgaaaa aatagaaata aaaaattagg aggaaaattt
      181 atgaaatatc aggtttcatt attagcagtg aaggatattg aagtatcaaa aaaattttac
      241 aaagaattat ttgagcaaga aattgaattg gacttaggta aaaatgtaac atttaaagga
      301 ggatttgcta tacaagaaga cttcgcatgg cttacaggga ttaatccaga tacaattata
      361 caaaaatcaa ataatatgga actttatttt gaggttgatg attttgacga atttattgaa
      421 aaactcaatt attataaaga ggtagagttt gtccataaac cactaaaaca tgaatggcaa
      481 caaagagtag tgcgtattta tgacccagat aagcatatta ttgagatagg agagtctatg
      541 gaagttatag ctagaagata tttatctgag ggatattctg tagaagaaac ttctaaaata
      601 attcaacatc caattaaatt tgtaaagcag tgtgaaaata gtaaggaatg attgtttaaa
      661 ttataattat ttagtagaaa ttaattgtgg tagaactaat tcaagataat atataggact
      721 agtatttgac aaatgtcata aatt
//
LOCUS       DAFKMX010000381          678 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.391, whole genome shotgun sequence.
ACCESSION   DAFKMX010000381 DAFKMX010000000
VERSION     DAFKMX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.391"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..598
                     /locus_tag="LZB08_003806"
     CDS             <1..598
                     /locus_tag="LZB08_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBM7317218.1"
                     /translation="FDYTSQSKTDLKKLNMYFFFSVFLIISSLYINIGANSRILICIF
                     VMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSNLDNMNVLLSGSLYR
                     YEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLSFFVLYDYIINLVKI
                     SPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKIR"
BASE COUNT          246 a           58 c           83 g          291 t
ORIGIN      
        1 ttttgattat actagtcagt caaagaccga cttaaaaaaa ctaaatatgt atttcttctt
       61 ttctgttttt ctcattattt cttctttata tataaatata ggagctaata gtagaatctt
      121 gatatgtata tttgtaatgt tcgttttcta taagtctaac tataaggtta atatatataa
      181 atgtctggta atctcatttt tttattggtt aatattaata ggtataaatg gattaagtat
      241 gttattagta gcaacagtaa gcaatttgga taatatgaat gttttactta gtggcagttt
      301 atatagatat gaagcaatat tcttgggaaa gataatgtta ggtatattac tttatgttta
      361 taaggcttct tttaaaaatt ttaaaataag taaaaaagag atatacatat gtattcctat
      421 gatagcaaat ttattgtcat tttttgtact ttatgattat attattaatc ttgtaaaaat
      481 ctctcctgaa agaagaactg cttttttagt aatagtttta ctattaatat tgtctaacat
      541 ttcaatcttt gtattaatac atataatact taagattaaa attaggaaaa taagataaat
      601 tttaatacga ttcatttaaa ttaaaagtta ataattactt ttttatgtat aattttacat
      661 gaatttctat aaaagtta
//
LOCUS       DAFKMX010000382          652 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.392, whole genome shotgun sequence.
ACCESSION   DAFKMX010000382 DAFKMX010000000
VERSION     DAFKMX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.392"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            306..527
                     /locus_tag="LZB08_003807"
     CDS             306..527
                     /locus_tag="LZB08_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317219.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          295 a           53 c           96 g          208 t
ORIGIN      
        1 cttaaaacca taaaatacac caactataat gatgagattt ccttacatcc tccatataag
       61 tcatcattat agtaacccaa caattaagtt actatgaatt taaaaaataa caccgagcat
      121 ataatctatg cttagtgtta tttttatttt gaaataaaaa atatttaatt tattgaaatg
      181 tatagattta gatagtttaa actatattta agacttaaaa attataattg atattacaaa
      241 taaatatatg tactttggga aaaataaata taggaagtat tatatttaca gaggaggtag
      301 ttttgatgga tagattaaaa agagatatga aaaatatgaa agaagattta aaagaaggtg
      361 ttaaaaagac agcttatgtt atgaaaaata cagctgaaga tttgaaagat gatgcacaag
      421 gtgctatggt aaacttagaa atgaaaaaag atgagatgat ggaaagatat gaacaaaaga
      481 aattttctaa agaattagag aagcaaatga agaatgaaat gaaataagct tttatttatg
      541 aataacaaat tattgtattc atataaaaag tagccagtaa tattaaatag tttatttaag
      601 gaaatagact ttctaaaaat aactgtatta gatttagaaa gtctatttca aa
//
LOCUS       DAFKMX010000383          646 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.393, whole genome shotgun sequence.
ACCESSION   DAFKMX010000383 DAFKMX010000000
VERSION     DAFKMX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.393"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            103..219
                     /gene="rrf"
                     /locus_tag="LZB08_003808"
     rRNA            103..219
                     /gene="rrf"
                     /locus_tag="LZB08_003808"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            339..530
                     /locus_tag="LZB08_003809"
     CDS             339..530
                     /locus_tag="LZB08_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBM7317220.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
BASE COUNT          227 a           69 c          119 g          231 t
ORIGIN      
        1 gatatatgtg tagtttttag agtgctaact ctaaagaaac tagtattact agattctata
       61 aacgtattaa aaacttaata gaaatattaa gcataaagat tatgtggtta caatagcaga
      121 gaggatacac ctgttcccat tccgaacaca gaagttaagc tctctagcgc tgatggtact
      181 tggtgggaaa ctgcctggga gagtaggacg tagccacgta atttttttta ttttttgtca
      241 aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca caggaattat
      301 ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt gctttaattt
      361 tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca gacttaatag
      421 gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc attgtaggtt
      481 tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa tttatgcttt
      541 tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat aaaaatatta
      601 atttgtttct tttttaatgg taaaatagat aagaattgaa gtttta
//
LOCUS       DAFKMX010000384          606 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.394, whole genome shotgun sequence.
ACCESSION   DAFKMX010000384 DAFKMX010000000
VERSION     DAFKMX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.394"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(42..482)
                     /locus_tag="LZB08_003810"
     CDS             complement(42..482)
                     /locus_tag="LZB08_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBM7317221.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT          227 a           71 c           51 g          257 t
ORIGIN      
        1 aataattgta actagataat attcaatttc tatattaata tttaactaat attagataat
       61 gtatcgttta tatccttttc tactaatggt ttcatattat tattataaat acttatatct
      121 gcattactta attccatttt tatgcgatta ataagatttt ctttatactt tgtaatatta
      181 ggtatggatt taataaacat tcttgataca gaaggttttt tatctacgat atgacttagt
      241 atactatcta tatttatttc aattttatta tcaatatccc actgagcatt tgcagagatt
      301 aataacaatc cccttgttcg aatatatgag ttttcgttat caatcatttc taggaatgag
      361 tcaaaaaaac aatatgtaat atttgatttt aaactttcat tttctaagaa taaaaaagtt
      421 ttatatgctt cattatgatt ttttgaatat aatttattaa taagtaaatt gagattcttc
      481 aatcttctca tctccctcat tacaaatatt tatttaaaca aagtataaca tattcaaaaa
      541 taaatataaa taaaaactat attaaataaa aagcctaaaa ttatttgctg tttatttaca
      601 aataat
//
LOCUS       DAFKMX010000385          571 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.395, whole genome shotgun sequence.
ACCESSION   DAFKMX010000385 DAFKMX010000000
VERSION     DAFKMX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.395"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(72..551)
                     /locus_tag="LZB08_003811"
     CDS             complement(72..551)
                     /locus_tag="LZB08_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBM7317222.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEHSLFVKENVKRFRTIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          229 a           98 c           47 g          197 t
ORIGIN      
        1 taatatattt taaattacta gttaatcact cttttcgggt tatttatctc ctatatatta
       61 aagtgtcaca cctaaatagt taaatcatta tcctctttta tctttattgc ctttacaaat
      121 ccatctgcaa ttacaaaact cataagagaa gttataaaat atacaaatac tggaacagta
      181 aaataaaatc caaatcctaa atctagaaat ctcattcctt ttccagtagt accaaaagtt
      241 gaaataaact caattagaga gtttaaaagc aaaagatatc caatagtcct aaatcttttt
      301 acattctctt ttacaaataa gctatgttca gaactaccta atacttctat caatgatttt
      361 ataatgataa aatatgatac acctaaaata aacattaata ttccataaat agtaccttgt
      421 agagttgctt ctccactaaa acagaggtac ataaaacttt taaatcctcc tgcaacaatt
      481 actaatccta aaagaaaaaa taaaatttgt aatataccac ttagtaaatt ttttataagt
      541 ttattagaca tcatgccaac tcctttaaaa t
//
LOCUS       DAFKMX010000386          560 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.396, whole genome shotgun sequence.
ACCESSION   DAFKMX010000386 DAFKMX010000000
VERSION     DAFKMX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.396"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            30..>560
                     /gene="rgbR"
                     /locus_tag="LZB08_003812"
     CDS             30..>560
                     /gene="rgbR"
                     /locus_tag="LZB08_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBM7317223.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEK"
BASE COUNT          270 a           40 c           92 g          158 t
ORIGIN      
        1 taactagaaa ttatgtaagg aggcatttta tgattaagat agcagtttgt gaagatgaaa
       61 aagaaacaca acttttaata gaagattacc ttgagaatat attaaaagat ataagtatag
      121 aatatgagat acaaaagtat atatctggag aagagttact ggaaagtaat ttaaaagata
      181 tagatatatt acttcttgat ataaaaatgg aaaaactaaa tggaatggat actgcaagaa
      241 agattagaga agtagacaat gaaatggaaa taatatttgt aacttcatta atagattatg
      301 tgcaagaagg ttatgaggta agagcttata ggtatttact aaagccaata gagttggaag
      361 aactaaaaaa acatgtgcta acttgtatca aagatattga aataaataag gaaagtcata
      421 ttacaataaa aaataagtct aatacatata agatttattt aaatgaaata aaatatatag
      481 aagttcaaaa aaaagatatg ctaatacaca caataaacaa gaattttgat ataaaatata
      541 gtttaagcaa aatagaaaaa
//
LOCUS       DAFKMX010000387          533 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.397, whole genome shotgun sequence.
ACCESSION   DAFKMX010000387 DAFKMX010000000
VERSION     DAFKMX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.397"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            313..>533
                     /locus_tag="LZB08_003813"
     CDS             313..>533
                     /locus_tag="LZB08_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBM7317224.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKF"
BASE COUNT          210 a           54 c           49 g          220 t
ORIGIN      
        1 aagtttaatt tcttcattaa ttttatcaac tatgcctcta ctgcctatta atttaatgca
       61 ataaataata aatcttttac aaaaagataa taaaaatata gaaaaatatt ttttgttgtc
      121 cttttggtta tggtaaacca cattctgcct gcaatataac taaatatatg ggtagatagt
      181 aaaattaatt ttattattta tatataattt tataagttac ttttcaataa tattgactgt
      241 catgaattta taatattcaa taaaatattt taatttagtt aagaatctat attgtaaagg
      301 agtattaaat taatgaactt aaaattaata tggataaata ttctaaaccc agtatttatt
      361 aaaaatgaaa taaaaagaaa aaaaatacaa attattatct ttttctttat aaactttggt
      421 ttaacttatt cattaggttt actgttatat tataataaat ttattgatcc agaaaacttt
      481 gcatcattca tgatgatatt acctctatct tcagttgcta ttgcaaagtt tta
//
LOCUS       DAFKMX010000388          523 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.399, whole genome shotgun sequence.
ACCESSION   DAFKMX010000388 DAFKMX010000000
VERSION     DAFKMX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.399"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
BASE COUNT          232 a           41 c           61 g          189 t
ORIGIN      
        1 attattttaa tggagaggat aaaaatgata agataaaatt tatgactata aagattttta
       61 tatttttgtc attaccatta tgttttaata tactattcaa ttatttaacc tcttaaatta
      121 tatttagata taggagtctt atgataaaag taagcttatg tgttatttat attaaatata
      181 aagtgcatat agtgataaaa caaaagtttc atataaatga agaaaaaaat ataaaataat
      241 ttatttaact acttaaaaat aaactaaaag ggataataaa atagctttta ggttatggta
      301 ataaaggtat ttaaaaagag tgcataagca ctccaattgt gagaaataca tacacaaatc
      361 attattacaa caatgaaaaa tgtacatata agagacgttt tgtatttgtt ttaagtgata
      421 atattttaat actaactaaa taattatcac aatagtaaaa aagaaaaagg agtattttgt
      481 actccatttg tcaaaaatat aaaactttta tataagctta tta
//
LOCUS       DAFKMX010000389          475 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.402, whole genome shotgun sequence.
ACCESSION   DAFKMX010000389 DAFKMX010000000
VERSION     DAFKMX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.402"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            108..392
                     /locus_tag="LZB08_003814"
     CDS             108..392
                     /locus_tag="LZB08_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317225.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          211 a           28 c           82 g          154 t
ORIGIN      
        1 attttgtttt tatataagag aatttgaata atatggaaaa agatgattaa taaattatta
       61 attagggtat aaaatgatat aaatagaata aataaggggt ggatgaaatg cttgtatata
      121 ataaaagttt ttatcctaat gacatatttc caagattaga tttttcaaaa ataaaaaaac
      181 agttaaaatt gatagataat gacctgtcag attttggaag catatgtata atagaaaaag
      241 aacattatac gataagtgta aacagtatag gtgaaataaa tgtgtattat gatttagagt
      301 acgaaaataa ggtgtataga atagtttatg agattgaaaa gttatttaaa tctcaagttg
      361 gaaggtttag tatatctaca tacagaaatt gataattaaa aagtagaaat taaaaaactt
      421 gatactaaag atatagataa atatatgagg taacagctag agagagttgt taccc
//
LOCUS       DAFKMX010000390          446 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.403, whole genome shotgun sequence.
ACCESSION   DAFKMX010000390 DAFKMX010000000
VERSION     DAFKMX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.403"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..>446)
                     /locus_tag="LZB08_003815"
     CDS             complement(<1..>446)
                     /locus_tag="LZB08_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBM7317226.1"
                     /translation="NHLNELLVLLNMSLCVEISALYQTKTDVIALVEEVSFFKTLKNK
                     ALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFCKCIISLCYLES
                     GNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNK"
BASE COUNT          157 a           77 c           34 g          178 t
ORIGIN      
        1 ctttattcat atcttctttt aaatatgccc atataaggtc tatttttata gacactcttt
       61 cagttactga tattgagtta ttactgtgtg caacatctaa tacattattt atgtatttta
      121 tagcttctac attatttcca ctctctaaat aacacaaact tattatacat ttacaaaaag
      181 cctctataga gtataaatta ttttcttgag ccaattttag acattcaatt aagtattcca
      241 tactttttat aaagttatta aataaatata gataaactct cccaaatata taatttgccc
      301 tacttacaag agctttattt tttaaagttt taaaaaaaga cacttcttct actaaagcta
      361 ttacatccgt tttggtctga tataaagcac ttatttccac acataaactc atatttaaca
      421 ataccaacaa ctcatttaaa tgattt
//
LOCUS       DAFKMX010000391          438 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.404, whole genome shotgun sequence.
ACCESSION   DAFKMX010000391 DAFKMX010000000
VERSION     DAFKMX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.404"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..>438
                     /locus_tag="LZB08_003816"
     CDS             <1..>438
                     /locus_tag="LZB08_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7317227.1"
                     /translation="VLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFE
                     ENLIKDIIENLKIKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIE
                     DSLDIKNQVAKINFLVNSILYSSRSNTMFEDVFINKFNLEKLVK"
BASE COUNT          189 a           28 c           68 g          153 t
ORIGIN      
        1 gtgctgttta taataatggt attttttgtt atagattata taaattttag aaatagttac
       61 acaaatttat ataattgtat agaaaatagt ggtgaaatag acagttattt agttgatgga
      121 caatcttttg aagaaaatct aataaaagat attatagaaa acttaaaaat aaaaaataat
      181 agtgacatag agacttataa acaatcatta aaagaattgg atgagtacat agctaaatgg
      241 gttcatgaaa taaaaattcc aatatcttct ttaagtataa ttacagatag gttaagtagc
      301 atagaagata gtttggatat taagaatcag gttgcaaaga taaatttttt agtaaactct
      361 attttgtata gtagtagaag caacactatg tttgaagatg tatttataaa taaatttaat
      421 ttagaaaaat tggtaaaa
//
LOCUS       DAFKMX010000392          422 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.405, whole genome shotgun sequence.
ACCESSION   DAFKMX010000392 DAFKMX010000000
VERSION     DAFKMX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.405"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..>422
                     /locus_tag="LZB08_003817"
     CDS             <1..>422
                     /locus_tag="LZB08_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBM7317228.1"
                     /translation="QNELYIIEVNPRASRTVPYISKVSGVPIVDLATKCMLGAKLKDL
                     GYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVLGVGENLEEALYKGFL
                     AAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYT"
BASE COUNT          164 a           45 c           94 g          119 t
ORIGIN      
        1 caaaatgaat tatatataat tgaggttaac cctagagcta gtagaacagt tccatatata
       61 agtaaagtaa gtggagtacc aatagttgac ttggctacta agtgtatgtt aggagctaaa
      121 ttaaaggatt taggatatgg tacaggagtt tacaaagagc caaaattagt atcagtaaaa
      181 gtaccagtat tctcaatgtc taaattatct aaagttgaag taagtctagg acctgaaatg
      241 aagtctacag gagaagtcct aggtgtagga gaaaatttag aagaagcctt atataaaggg
      301 ttcttggcag caggtagaca tatgtctgat gaaagaggag tagtacttgc tactgtaaat
      361 aatcatgata aagatgagtt tatagagata gcaaaagata tgaaagaatt aggatatact
      421 tt
//
LOCUS       DAFKMX010000393          419 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.407, whole genome shotgun sequence.
ACCESSION   DAFKMX010000393 DAFKMX010000000
VERSION     DAFKMX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.407"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            157..390
                     /locus_tag="LZB08_003818"
     CDS             157..390
                     /locus_tag="LZB08_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBM7317229.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
BASE COUNT          185 a           31 c           72 g          131 t
ORIGIN      
        1 aaatataata tggtaaatgt caaaattata aaaaaattct acttatataa aatataaaga
       61 ggttatatgt acatatgtgt agaaataaat aataaaaata tcaattaatg ttattagggg
      121 gggttactca ttagtaaatt agctaataca cttagaatga tgatattaat tcaaagcaga
      181 ggcaaaatga aatgcaaaga attatcagaa gagttggaag taagtgaaag gcaaataaaa
      241 agttacaaga tgtatttaga gcaagctggt atatttataa attcaacacc tgggatttat
      301 ggtggttatg aaatagataa atgtaatagc atatcattaa ttaaattatt agatagtgaa
      361 gtttcaattt ttatatcaac taagtggtag ataagtagaa tattagcaat atcaacgag
//
LOCUS       DAFKMX010000394          410 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.408, whole genome shotgun sequence.
ACCESSION   DAFKMX010000394 DAFKMX010000000
VERSION     DAFKMX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.408"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..346
                     /locus_tag="LZB08_003819"
     CDS             <1..346
                     /locus_tag="LZB08_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBM7317230.1"
                     /translation="YNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDK
                     LKDIEEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYI
                     NKCNNLNVVNKI"
BASE COUNT          194 a           20 c           61 g          135 t
ORIGIN      
        1 ttataatatt gtagattttt ctgttgaaat aataaaaact gtgagaatgt acttattaac
       61 aatcatagat gtaaatatac aaaatttaga aattatgaaa gatataagta aaaaaatgga
      121 tactatagat aaattgaaag atatagagga aggctatttt tatttattag aagatgtgta
      181 cttcgatatg aaagatgtac tagaagattt aatgctttat agaatgaatg aagatgagta
      241 caagtatgag ataaaaaata taataaaaat gtttgatgaa gacttagata atgaaaaaat
      301 atatataaat aaatgtaata acttaaatgt tgttaataag atataagaaa ttataaataa
      361 tagagtagaa cttgacaatt ataacaattt tttataaaac tgaatcaaga
//
LOCUS       DAFKMX010000395          380 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.410, whole genome shotgun sequence.
ACCESSION   DAFKMX010000395 DAFKMX010000000
VERSION     DAFKMX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.410"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     regulatory      133..223
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          175 a           37 c           41 g          127 t
ORIGIN      
        1 attaaatgtt ataaatataa atatattaaa aatattataa aatttaatat tagaaattaa
       61 attaaaaaat tatataaaaa aatacttaaa aaaatattta aaaaatttgt tatttattgt
      121 atacttaagt taaaactaaa taggcaaatc tagagaaatc tagtgacgca aagctatagg
      181 gactaaggtt tatatacata aactatgtca gccagttgcc aaaaagagtc ctaggtgtat
      241 tgtataccta agaaaagtct ataatgactg cctttttggc agtcattttg ttatggaaaa
      301 atcactttat ttcagtagaa aatacatata ataacatatt ttaccaatta taacataaaa
      361 taaagaaaaa ttatctcaaa
//
LOCUS       DAFKMX010000396          373 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.411, whole genome shotgun sequence.
ACCESSION   DAFKMX010000396 DAFKMX010000000
VERSION     DAFKMX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.411"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..>373)
                     /locus_tag="LZB08_003820"
     CDS             complement(<1..>373)
                     /locus_tag="LZB08_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317231.1"
                     /translation="INTLYEEYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNF
                     NLRPDIVLYDLKNEYEIIIDTKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILL
                     YPCIQKDKNYSSWKLSESFKGK"
BASE COUNT          120 a           57 c           31 g          165 t
ORIGIN      
        1 cttaccctta aatgattctg ataatttcca ggaactataa ttcttatctt tttgaataca
       61 aggatataat aatatacatc tttttgcatt ttcatatgct gttatatatg catacatctg
      121 gtatatatca cttgacctat aaaaaacatt tgaatctact tcaatagctt tccatttagt
      181 gtcaattata atttcatatt cattttttaa gtcatataaa actatatccg gtcttaaatt
      241 aaagtttttc ttacctgttt gttcattttt caaaagaaat ttactcttat cttgtatata
      301 tgtttctcta aatgaattgt cccatatact tttgattaaa attcctatat attcttcata
      361 caaagtattt att
//
LOCUS       DAFKMX010000397          373 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.412, whole genome shotgun sequence.
ACCESSION   DAFKMX010000397 DAFKMX010000000
VERSION     DAFKMX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.412"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="LZB08_003821"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="LZB08_003821"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="LZB08_003822"
     tRNA            300..371
                     /locus_tag="LZB08_003822"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           60 c           80 g          115 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgta gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAFKMX010000398          360 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.413, whole genome shotgun sequence.
ACCESSION   DAFKMX010000398 DAFKMX010000000
VERSION     DAFKMX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.413"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            129..245
                     /gene="rrf"
                     /locus_tag="LZB08_003823"
     rRNA            129..245
                     /gene="rrf"
                     /locus_tag="LZB08_003823"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            251..339
                     /locus_tag="LZB08_003824"
     tRNA            251..339
                     /locus_tag="LZB08_003824"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Leu,seq:taa)
BASE COUNT          122 a           63 c           81 g           94 t
ORIGIN      
        1 aaacttatgg atttttaata gttttataag gcaaaaaatc gaataaaaaa agtattgaca
       61 caataagaca aagatgttat tataatactt gtcttagaaa aaaagttaaa tgaaaattta
      121 gacaataatc tggttataat agcagagagg atacacctgt tcccattccg aacacagaag
      181 ttaagctctc tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc
      241 caggtgatgt gccgaagtgg cggaactggc agacgcacag gacttaaaat cctgcgggac
      301 ttacctctcg taccggttcg attccggtct tcggcaccac aaattattgt gggccattag
//
LOCUS       DAFKMX010000399          349 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.414, whole genome shotgun sequence.
ACCESSION   DAFKMX010000399 DAFKMX010000000
VERSION     DAFKMX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.414"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            90..278
                     /locus_tag="LZB08_003825"
     CDS             90..278
                     /locus_tag="LZB08_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317232.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCEKGQKKFSEETSHHTCFKNII
                     EAIHISKSLIMDEISKRD"
BASE COUNT          148 a           26 c           54 g          121 t
ORIGIN      
        1 tgtataaata ataatgatgt cagtttataa tgatattaaa ttgaggtata ctgtttctct
       61 aaattaaaaa ataaaggagt aaatgtgtta tgaattatca tacaaaagaa gaattggtgg
      121 aagcactgag agtagtatct tcaagtatta ttaattgtga aaaaggacag aagaaatttt
      181 ctgaggagac atctcatcat acatgtttta agaatattat agaagcaata catatttcaa
      241 aatcattaat aatggatgaa attagtaaaa gagattgaag cttattttgc aatttaacat
      301 gttttaatat acattttatt atataagaaa ataagtaaat atacatgtt
//
LOCUS       DAFKMX010000400          344 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.denovo.415, whole genome shotgun sequence.
ACCESSION   DAFKMX010000400 DAFKMX010000000
VERSION     DAFKMX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.denovo.415"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..>344)
                     /locus_tag="LZB08_003826"
     rRNA            complement(<1..>344)
                     /locus_tag="LZB08_003826"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           77 a           95 c           59 g          113 t
ORIGIN      
        1 gtggtgggtc tttccaaacc tcttcaatta cgatacctct tcgttatgat atgtccgcaa
       61 ccccaataaa gaaaacttta ttggtttggg ctaatccgcg ttcgctcgcc gctacttacg
      121 gaatcgaatt tctttctctt ccttcaggta cttagatgtt tcagttccct gagttcccct
      181 cattaagcta tgtattcact taatgatact tagacattac tctaagtgag tttcctcatt
      241 cggaaatctt cggatcaaag tttacgtgca actccccgaa gcttatcgca gcttatcacg
      301 tccttcatcg gctcctagtg ccaaggcatc cgccctgcac cctt
//
LOCUS       DAFKMX010000401         9217 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.158, whole genome shotgun sequence.
ACCESSION   DAFKMX010000401 DAFKMX010000000
VERSION     DAFKMX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.158"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..224
                     /locus_tag="LZB08_003827"
     CDS             <1..224
                     /locus_tag="LZB08_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317233.1"
                     /translation="IEREVNIFEDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHS
                     TVIDDDRYKCCNVQYTKNRVIILYYIYNH"
     gene            complement(552..1319)
                     /locus_tag="LZB08_003828"
     CDS             complement(552..1319)
                     /locus_tag="LZB08_003828"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBM7317234.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEVKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(1991..2806)
                     /locus_tag="LZB08_003829"
     CDS             complement(1991..2806)
                     /locus_tag="LZB08_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBM7317235.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHKDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSARAEMERL"
     gene            2907..3605
                     /locus_tag="LZB08_003830"
     CDS             2907..3605
                     /locus_tag="LZB08_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBM7317236.1"
                     /translation="MYKLSYVVRVSTKDLDELKRRCYEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLNTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAKRGKW
                     KETLPEIAHEINIVNLTSDDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            3693..4313
                     /locus_tag="LZB08_003831"
     CDS             3693..4313
                     /locus_tag="LZB08_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBM7317237.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4463..4867
                     /locus_tag="LZB08_003832"
                     /pseudo
     CDS             4463..4867
                     /locus_tag="LZB08_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            4992..5918
                     /locus_tag="LZB08_003833"
     CDS             4992..5918
                     /locus_tag="LZB08_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317238.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            5932..6381
                     /locus_tag="LZB08_003834"
     CDS             5932..6381
                     /locus_tag="LZB08_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBM7317239.1"
                     /translation="MELIEWNYTYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRESSKVLEKCGFKIEEYCHNS"
     gene            6741..7148
                     /locus_tag="LZB08_003835"
     CDS             6741..7148
                     /locus_tag="LZB08_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7317240.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            7150..7380
                     /locus_tag="LZB08_003836"
     CDS             7150..7380
                     /locus_tag="LZB08_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317241.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            7770..7973
                     /locus_tag="LZB08_003837"
     CDS             7770..7973
                     /locus_tag="LZB08_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBM7317242.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            8051..8404
                     /locus_tag="LZB08_003838"
     CDS             8051..8404
                     /locus_tag="LZB08_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBM7317243.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKSQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            8476..9108
                     /locus_tag="LZB08_003839"
     CDS             8476..9108
                     /locus_tag="LZB08_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBM7317244.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3304 a         1366 c         1444 g         3103 t
ORIGIN      
        1 aaatagaaag agaagttaat atttttgaag atgaggatgt tataataaac aaaaaagtat
       61 tgaagtttcc aaagtcttgg acaaagaata tgcaaaaata ttggttaagt gaaaataagt
      121 atcccataca ttctactgta attgatgatg atagatataa atgttgtaat gtacaatata
      181 caaaaaatag agtgataata ttatattaca tatataacca ttaatataca taattacaaa
      241 aaaataatga caaatataca taatacaaat atatcttcta ctttagataa aacagttatt
      301 atgaaaactt actatctata aataatagaa atgctagtat accatctata atctaatgtc
      361 tagtaggttt taatggcata ttttaaatat gccatagttg atttataaga ttttctatat
      421 cactttcaaa atacacacta aatagttgct tttcagatat aattgcccaa tttaaatttt
      481 actatctgaa aagtttttta tatttaattt atatcttcaa aaataccgtc taatatttct
      541 atataattaa tttaattaat actcttatgt aatagattaa gtactgaatt taactcttta
      601 aatgatattt gccctggtgc tgaaacactt tttgcagctc caaaagtcaa tgcagaacca
      661 aatatttcac cacacaatct acttataaca cccattcctg acattgacat tgttattatt
      721 ggtctatctg cataaatttt aaacatttca tttgtagctt caagtagcac taacacatct
      781 ttttcatttt gcggcataac agctatttta ggtaaatctg cgcccaattc ctgcattcta
      841 cataaacgag atacaatctc ttcttttttt ggagttttat taaaatcatg attagatata
      901 attactttca cttctttttt atgagcaaaa ttaactactt catctatgac ctcatctccc
      961 ataaataact ctacatctat taaatctact aaacctgtat tagaaatttc tttattcaaa
     1021 gtagtataat aatctcttga tattaatttt tctccccctt ctactacact tctaaatgtg
     1081 aataataaag ggatatcatg tatataactt cttaattcat agagaacttc cttaacttct
     1141 ttaatatttt ctacattctc aaaaaaatca actcgccact ctataatatc caaacatgca
     1201 tcttttaact ctttaacctc ttttataata tcttttttat ttttcccaat aattggtaca
     1261 caaattttag gtctaccttc tccaatagta atatttttta cttgaacttt tcttttcata
     1321 ttctcctcca taatcagaaa ttctatagga ctttaaattc tattttctgc acaatttaat
     1381 tatacattaa aaatactata cattataaaa atactaattt aaaaataatt tttattctaa
     1441 ttcttaaaac aatcccacat attttagaaa aggtactcta taatatacaa acacttcttt
     1501 tttacctaaa tttattttaa cattctataa ttttcacaat attctttaaa ttttattcat
     1561 ataaacaata ttattctgga tgctaataga attaatctat aaatcatagt ataattatgc
     1621 agaaataact cgaatttcac tacttatgaa gagattgaat attaatgaat ctaaatcatt
     1681 tgcattactt aagagttttg actaaagttg aacaccatac aaaagtagct actcaattgt
     1741 ttatacccca accaagctta agtaatgtta tatagattct cgaaaaagaa tttggtatct
     1801 ttttatttga aaaacttata ttaaatttga ttatcttttc ataagttggt aaatgtcgat
     1861 atatcctgta tctacaagga gtatcggcat tttctttttg gaagatactt tgcataagtt
     1921 ggcatttaac cgtataagaa tgccatcaaa agtagtaaat aagtagtaaa ctctagtcat
     1981 actactaggc ttataaccgt tccatttcag ctctcgcaga actaaatgaa ccatgtgcat
     2041 aatagccaag cgtcatagta atgtttttat gccccataat atattgcaat atattaggac
     2101 tcattccttt gttagccata tttgtacaga gtgtatgcct aaaggtatgt ggcgtaatct
     2161 ttggtagctt gtccttatgg gtcttgttat acttcttgac taacgctctt aacatgcttt
     2221 gataatgagt tgcttgtttt ggtaatccat cttgtttaag aaataggaag ttctggtaac
     2281 catcaacaat gattggttct gctttagcgt tcttacgccg ttccacgata cgttcaaatg
     2341 attgataagc tctttcagtc attggaattt ctcgcttacc ctgtgtggtc tttggaactt
     2401 ctacataata gccaatctta gtatcactca acaattgatg gtctaccata atggctttat
     2461 ttttcatatc caattctaaa gtcaaaccgc ataattcaga aatgcgtagt cctgtttcta
     2521 agaggataac aacatcatca taatatttac aataagtctt atcgctctcc ataaaggata
     2581 ataagcccat ttcctgttct ggtgttagag cgatttttgg ttctgttgta tcttccaaaa
     2641 catcacttaa ctgaaaatta aatggattct ttctaataca gtcgtcttga attgccatga
     2701 aaaacgacgc tttcagtgag cgcttataat tgtcaatgga tttataagca cacccatctt
     2761 cactcattcg gatagcccag cttttagcgt ctgataattt aaccatatca atgctttttg
     2821 cacctagtgg gtcattgata atatctatat tttgatttat aatttataca tttttttaaa
     2881 aacagacttt taactgtaaa gaatctatgt ataaacttag ctatgttgtc cgtgtatcta
     2941 caaaggatct tgacgagcta aaaaggcgtt gttatgaagt caaagacttt tacgacgact
     3001 tatcaatcaa gctcgtgcgt ccctttgggg atatgttggg attgcatggc gaatttattc
     3061 ctgtcagcaa acgttacatc aatgattata ttcaatatgt cacttcggat tttttggctg
     3121 gacttggttt tggagcgaca caaacccttg gcgaacatga ggatatctat cttggctaca
     3181 atctgaacac tggaaagaat gtctatctaa aacctgccct tgctagtcaa ggggtaaagg
     3241 gttctattac caatgcgtta gcgtctgcgt ttatcggttc attgggtggt ggtaaattat
     3301 tcagtaacaa tatgcttgtt tactatgcgg tactattcgg cggacaagcg gtcatagttg
     3361 accctaaagc aaaacgcggc aaatggaaag aaacgctacc agaaatagcc catgagataa
     3421 acattgtcaa tttaaccagt gatgatgaaa acaaaggatt gcttgaccct tacgtcattt
     3481 tgagccgtaa aaaggattct gaaagtttag cgattgatat cctaacattc cttactggta
     3541 tctccagtcg tgacagtgat aagtttttgc agaaaaatga caactggaaa ataataatgg
     3601 aatagtttct atgtaaatat atttttacta tcttacctaa ctaattgaaa gatgatataa
     3661 tttaatcaaa gatttggtaa ggagagatga atatgcattt aattgatttt atgaatgaag
     3721 atattaaaaa gaagcttgtt aaaaaaacgt ataaaaagaa agagactatt ctttttgctg
     3781 agcaagaaaa tgaatatgta attcttatga ttgacggtat tgcagaagca ttcatcttaa
     3841 atgtgaaagg aaacatctca actattcatc tttatagaag tggtagtttt tttggagaaa
     3901 tggaacaatt taatgatggg aaaaaacccg ttgaaattgt cgctttcaca gattgtaatg
     3961 tatataaact tcatagagat gattttttaa actggttaag gggagatttt gaagctacaa
     4021 aatttctgat tcgagaaata tctagtaaat tagttgtaaa tgctgaatta attgaagaat
     4081 tatcatcttt aagtgttaaa gaacgacttc tgaggtgtat atccttacac tattatagaa
     4141 gtgaattaaa tctgcttacc aaaaaacagc ttgctagcga agtaaacgct ccgattagaa
     4201 gcattaatcg tgctataaat gaatgtgtac aagaacatct aattgcttat gaaaacaaaa
     4261 gattcagtgt aattaatcag cgagaggtat taaagaatct tcctacaaca taagtataat
     4321 ttcaaaaaag tattgaataa taattattca atgctttttt atttcgggtc aagtgaccta
     4381 ttttttttta tttcttcatt atataatact cctatgcata attatgttgg attgatagct
     4441 aataaaagaa agtgagaggt gttatactaa aaaagaagca tacttcccaa ctccagactt
     4501 acagtggatt gaagatagat tttggatttg ggttcattac gcttgcacta agattggacg
     4561 tagagaacta tttgaaacaa ttgatttttt atctttttta agacaatcag tacttgcctc
     4621 gttaatacag cttctcttag gtaaattacc tcgtggagtt agaaaaattg aattggacgc
     4681 accccaatat attgaagcat tagaaaagac ggttgctaac catgaatatg aaagctgtgt
     4741 taatgcattg caggctacta tcaacctgta tcttgagtta agagaggaat tatcacaaag
     4801 tcctattgtc cgaaaggttg aagctgaaag ggttgcatta cagtattttc aagatttaat
     4861 caaataaacc caaagtaaaa aaatattgaa tagtaaatat tcagtgtttt ttcatttttg
     4921 gtcaaatgac ctagattagt tgttgtaacc attctataat tattttgaaa gtaacagtat
     4981 ggaggataac tatgagtgat attttaagta gaattacaaa taaaattggg gataagaata
     5041 tagttgataa gttatcagct ctatcaaaat cagatttgaa ctcacttcta ttagaagtgt
     5101 ttgatagaca agcaaatact ttaactgcaa ctgatattct aaagtcatac caactaaata
     5161 gatttactat ccctagcggt atagatccag aagaaattca tgctttggag tcaaaattat
     5221 tgagaaaagc attcaatatg gatatcaaga cgattatgct ttctccctct gctccattag
     5281 gtagctgtag cgtttttggc tccgtagatc aatataatgt agtaagtgct ctacgaggga
     5341 cagaaacact tgcagacccc tcaaatatgt tggcaataat tattgctgat aaattaaaaa
     5401 gaaaagaaga aaataatacc atacctatcc acatggctac tactgctagg gtaataagag
     5461 cacaaaattt tgtaggtaaa ggtttatatt cacattttgg cttatattgt atggtttcat
     5521 ctggtataga tacagggtct tacacttgtg aaaaaatgtt gttagaaaag cacttaaact
     5581 attataaaga tatattaagt gaaatagaaa atgttcgttt ctctattatt ctacgcaaaa
     5641 gaaacggata caaagataat gacggatttt tcgacagaat ggttgaaact atcaaactta
     5701 tttttcctaa tatagagtta tcaattcata atgatgatgt tgataataat tattataaag
     5761 gtatcaattt caaattatcg gtcaaacaag gaaatgaaac cgttgaaata gttgacggtg
     5821 gatttgttga ctggacttat caaatgcttg gaaataaaaa agaacgctgt ttgataagtg
     5881 gtattggctt agaaagattt cttgtagctg tttcataaaa ggagaagaaa aatggaactt
     5941 atagagtgga attatactta tattaccgat ttagttgagt ttgcgaatga taaagaaatt
     6001 gcaaagaatc ttcgtgacaa cttcccccac ccttataggg agcaaaacgc tagagatttt
     6061 attagttttt gtttggcaac tccagatgtt aaacaactta gctatgctat tttttatcaa
     6121 gaaagggcta ttggaagtat cagcctaaca tttgaagaag atacctctat taataaaaat
     6181 gctgaattag gttactggct tgctaggaac tattggggca aagggatagc aacaaatgca
     6241 gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc ttaacaaaat tcatgctaat
     6301 gtatattcct acaatagaga gtcaagtaaa gtattggaaa aatgtggttt taagattgaa
     6361 gaatactgtc acaattcata ataaagaata gaaacatgat tgattgccat gtatatagtt
     6421 taattcgacc gcaatagcat ttattagaat taacctactt tttaataaca gaataagttt
     6481 ttcccctcta ttaatttctt atgcatcttt gtatttttat gaaaagctag ccaatgcata
     6541 tttccataca tcaatatggt ggtggcttac aaccaatata aattaaatac gttttaaaga
     6601 atacacattt ttgccctata ggcagagtgt gtattttttt ctcttttttt tgaaatttat
     6661 cgaagattta acgccctccc accgtgatag gagtaagcaa ggttatttaa ctccttgcga
     6721 tggaaagagg gtgaaatctt atggttgatg aaaagacaat tgaacatcaa ttcgacagtt
     6781 tctgtaaaac agttttgcgt aactatgcta gagatattta tgacgaaaat aaacgcagaa
     6841 atgattattt ggtatctctc gaatctttgt cacttgcaga attaagtaaa ttgagtattt
     6901 tagatgatta tgattctaac tacatctgta tggtttctta cgactacaac atacgaattg
     6961 aagatgtatt aatggcgcaa gcaatcggaa aacttacaaa aagaaaacaa gatataattc
     7021 tactttcatt ctttctcaac atgacaaatg ctgacatagc aacattgatg gatttagcag
     7081 aaaataccat acattatcac aaaacaaatg cactaaaaga gttgaaagaa ctcatgcagg
     7141 aacattgaaa tgaaacctac taaagaagta ttacctttta caattatttt acttgctgta
     7201 catggcgatg taaatgcaat aaacgtaata cttaaacatt ttgaacacta tatcattcgt
     7261 ctttcacaga aaactttatt tgatgaatac ggaaatccct atattcatgt agaagaagaa
     7321 acaaagcgaa tgctaggaac aaaactaatc actgctattt tgagatttaa tttgtcttaa
     7381 tctcgccctc tctccatgta tcaagctatt tggtacatgg agaatcacta attatttgtc
     7441 tataaaagcc cataagctat tgtgattttt tataggcaaa tcaatgccga ctgttctttg
     7501 acaactgaat aaagtagata gatacgtttg tgatacagat agccaacaaa tactatacgc
     7561 catgacctta atcaagagag ctactatata gtgatttgtg aataaccttt gtaacggttg
     7621 ttttgccgta acctgtacca cagtataatg atacacctgt atgacacggt tctgcccata
     7681 agaatgggat tggttgaaat accaatggag cttaccaaag ccatctatct acttctgaac
     7741 tacgaacata gaaaggagtg atacattcta tgaacaatac tgatgtacct atttgggaaa
     7801 aatatacctt gacgattgaa gaagcgtcaa aatactttcg tattggagag aaaaaattac
     7861 gtaaacttgc tgatgaaaac cttgattctg gctgggtcat tatgaatagc aatcgcattc
     7921 aaataaagag aaaacaattt gagaaaatcg ttgatacact tgactttatc taatgaaatt
     7981 gagccttata tgttataata ttcatatgaa gtatcaagct caattcggtt attgagagga
     8041 gcttcttatc atgtcaactg aaaaaagacg agataataaa gatagaattt taagaactgg
     8101 agagagccag agaaaagatg gaagatacgc atataagtat gtatattcat ttggtaaatc
     8161 acaatttgtt tactcttgga aacttgtacc tactgataaa cccccaaagg gaaaacgtga
     8221 tgatatatca ttaagggata aggaaaaagc aatacaaaat gaccttgatg atagcattga
     8281 cactattggt aagaaaatga cagtttgtca gttatacgct aaaaagaatg tactaagaaa
     8341 gaatatccga cttaacacta aaaaaggacg ccattatctt atgaagattc ttaacaagga
     8401 gtgattctgt gaagagatgt attgtagtag tggaatagtc aaaaacataa tatatattaa
     8461 aggagagatt taaatatgac tattccaagt tcaaataagg tatatccaag aagtaatgat
     8521 tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt tggagattac
     8581 acaatatata atgactttta taatgaccca agagattttg agaaaaacaa tgtattgtat
     8641 cattacccta taaacaatga taaattaata attggtaagt tttgctcaat agcatgtaat
     8701 gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac atatactttt
     8761 cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc ttgggacaat
     8821 aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat aattatgtca
     8881 ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac taatgacgtt
     8941 ccaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag atttaataat
     9001 gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa aaaaattcaa
     9061 tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata ttttataatt
     9121 tatttatatt aaactttact ttaataatca ttttataaga gctttttcaa aatttgtata
     9181 ttattttaat atatataaat ttaatacaac attaaat
//
LOCUS       DAFKMX010000402         8515 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.169, whole genome shotgun sequence.
ACCESSION   DAFKMX010000402 DAFKMX010000000
VERSION     DAFKMX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.169"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(<1..1693)
                     /locus_tag="LZB08_003840"
     CDS             complement(<1..1693)
                     /locus_tag="LZB08_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBM7317245.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYG"
     gene            complement(1707..2636)
                     /locus_tag="LZB08_003841"
     CDS             complement(1707..2636)
                     /locus_tag="LZB08_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBM7317246.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            2864..3052
                     /locus_tag="LZB08_003842"
     CDS             2864..3052
                     /locus_tag="LZB08_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317247.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            3045..3746
                     /locus_tag="LZB08_003843"
     CDS             3045..3746
                     /locus_tag="LZB08_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317248.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3748..4671
                     /locus_tag="LZB08_003844"
     CDS             3748..4671
                     /locus_tag="LZB08_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7317249.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4828..5751
                     /locus_tag="LZB08_003845"
     CDS             4828..5751
                     /locus_tag="LZB08_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317250.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVIVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5744..6481
                     /locus_tag="LZB08_003846"
     CDS             5744..6481
                     /locus_tag="LZB08_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317251.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            6492..7265
                     /locus_tag="LZB08_003847"
     CDS             6492..7265
                     /locus_tag="LZB08_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317252.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7806..8414)
                     /locus_tag="LZB08_003848"
     CDS             complement(7806..8414)
                     /locus_tag="LZB08_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBM7317253.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2796 a         1413 c         1243 g         3063 t
ORIGIN      
        1 ttccatatat taatctttca gcttcaagtt ctgattcttt tcctgtaaat actcctaata
       61 ctaatatatt ttgctttcta gcttttctca cttcattggc tgtatctctc aaaccaacta
      121 tacccctata agccttttct cctcttaatg ttctctcact actttttcct atctttacat
      181 catttggtct tccatcgctg agaactatca ggattttatt ttcttcatct ctatttatta
      241 gaccactaca tatagatttt attgccaaac catctctatt attaccagca caaaaatatt
      301 caaatatatt ttcactattt tttatactat catcataatc tttaaatctt tttaatattg
      361 tatagtctaa aaaactagaa aatcccatca ctctatttgg tatgccaact tctgttagag
      421 catttgcaat tatgtaagct tgaattgcca catttccctg atttctactt tgagagccac
      481 ttgcatccaa taatatatca ataacgtatt tccctttttc attttctata tctttataaa
      541 atattttatt attattactt ctaccaactc tccaagctct attggctaat atagtaccac
      601 aatcagaata tactctactt ttagcatttt cttctattaa aattcttgac agactttctt
      661 ttaatctctt tatttgctgt ctatacattc tagcatgttc tctaaatttt cctaaattat
      721 tttctttttg tcttgttaca tattttagtt gaaatacatt attacactca cttctaagaa
      781 ctccatctgt aaagtgtatt ctacaacctt catgaacatt tctacaattt tgtgtctcaa
      841 tttttttaat ttcaccttta ctcaaataag tttttccata ataatgttct atttttgaat
      901 atatcttatt tgcagtgtct tcatcaacat atataactcg gtctgctgaa ttgctacatt
      961 tagaatcatc aaaatcccct acaccttcta caagcatgga tgaagatatt gaatctatat
     1021 cactttcaat aatattttct tcttcatcac tataaagttc ttcatacatg aaatcagcaa
     1081 atgtatcaaa atttacttct acactttcta cattattttc atcaataaga ctattttctt
     1141 ctccatttaa tatgtattca aaataagtta aatatataac atctaatgct tttaaaatct
     1201 ccataatatc ttgattggta tctatatttc ttatatcatt aactattctt ctagtcatac
     1261 tatctaatgc tgggtgcttg tccatactat aaagtacaaa tgtgtattta attttatcca
     1321 taatattatc attgtgtatt ttggtaaaac gtgaaacaat atctttataa gcatttcttc
     1381 ttatgtcttc tatccctggc ctgtctttaa tgaccttttc ttcaactatt gtatttaata
     1441 taacttgaat tagacctagt ataacatctt tgttgtatcc ttttcttatt ctatttgtaa
     1501 gatatttttt aactatatcc caatctacat actttcttct agcacctgca attattgcga
     1561 aatatagacc tatttctttt gaagagtaat ctttttctga tatatctata ctttcattgt
     1621 aatcaccagc tattgtccat gataggttat tagctctatt ttcaaattca taatcatcat
     1681 atatccattt cataatctca cctatattaa atatgactta ttggaaacac atctatactc
     1741 tcccatttat taggtattct agttttaatt acatctccaa ccatttcttt ctcatataca
     1801 tcaaaagtct ttcctgtaag tcccatatta attgcaagag ttggttttaa tcctcttctt
     1861 atagtcttaa gagatgccat aagaccacgt aaatcaatag ctttactaga tatctctcca
     1921 ttttgagact tcaattgtaa atctaaaaat attcctgcaa agtgtattaa tttttcttca
     1981 tcagcatctg aaaattcatt ttttaaaatc atcattaact tatcttcttc tataggagga
     2041 atatctatta ccataaatct agataccagt gcttcattaa gttcttttgt tcctgcatat
     2101 tcataattca tagtcccaat aaatcttgtt gctggatgta gattgactct ctcataccca
     2161 ggtacatcaa ttattcttct atagtctaaa gctgaatgta ccacaactat agcatcattt
     2221 tttgccatat taatttcatc aaatacccca aaacctccat taatagcaca ctcataaact
     2281 gagcctcttc taagtttaac ttcattgtct ataaatgtat ctgtacctat aagtgttgaa
     2341 ctgtctgtat ttatatgaaa tgatgtattc cactgaggtc tattaaatat ttcccctaga
     2401 ttatcagcaa gtaagttttt tcctgttgcc tttggtcccg ataataatat attttcttct
     2461 tctaaaatag ctgctataca catagacaag atatctttac cataaaaata agttttagac
     2521 tttgttactc tgtattctaa atctttttct aatccataat aatttctaaa atacagtaca
     2581 tcttcaataa gtttttcatc taccccttgc acccttagat tatctattat atccatattt
     2641 ttaccccttt tatataaata gtatttgttt catttcttat caccatttta gcataaaagg
     2701 atataagcta aaattgattt tttaatctcc ccattttaag taaaaccatg caccatttta
     2761 tcactacaaa attaagagta ctacttactg atatttcttg attttgttcg tctatacgta
     2821 tataatatat gctgataaat tatgaggaaa ggatgattga aatatggaat atatgtcttg
     2881 cccagaagta gcaaaaaaat ggggtatttc tgaaagaaga gtacaaaaac tatgtgaaga
     2941 aagtcgcata ccaggagttt caaaactcgg gtatatgtgg ctgataccaa agaacgcaga
     3001 aaagcctgtt gatggtagaa caaaaagaag aaaggaagta caatatgaat agcgttttga
     3061 ttatagatga tgacaaagaa ctttgtgctc ttatgaaaaa atgtgttgag caagaaaatt
     3121 tatctgctat tactgcacat tgtggtgcag aaggtctaca tttggtcgat aaaaatagac
     3181 acacactctc tcttgtaatt ttggacataa tgatgccaga tatagacggt tttcaagttc
     3241 tgaaaaaaat tagagaaaca agtaatattc cagtaattat gcttactgct aaaaatgacg
     3301 aggatgataa agtttccgga ctacgtttag gtgctgacga ttacctaaca aaaccgttca
     3361 gcataaacga acttatggct cgtgtcaatt ctcttattcg tcggtatact atgttaaacc
     3421 ctgcttttat aacagacaca gattgcataa taataaatgg catgattatt gacaagacaa
     3481 accgaattgt ttctgtcaat aatattcctg ttcaacttac aagcaaagaa tttgatttac
     3541 ttgcttttct ggcttctaat aagggacggg tgtttaccaa aaaacagatt tatacacaag
     3601 tatgggaaga agaatatgct tttgacgata gtaacatcat gtctttcatc agtaaattga
     3661 gaaaaaagat tgaaccagac cccaaccatc cattttatat tttgacagtt cgtggtgtcg
     3721 gttatcgttt taataaggag gcttaacatg aatacaagtg tctatctgct tttgatattg
     3781 gttcttagcc tgattattat agcgtacctt gtttctgtcc tgctacgtgt ccgaatacaa
     3841 cttacactta taaaagatgc tttagaggat ataaaaaatg gaaaccttaa tcgacgtatt
     3901 ttaacagatg aaaacgacat tacaaaacaa atttgctatg atattaacga aatagcaatc
     3961 aatagccaat ttcaacttat tcagcaaaaa caatcagaac aagcctacaa acagctaatg
     4021 acaagtctct cccatgatgt aaaaactcca cttgcttctt tggtcggtta tttggaagca
     4081 gtagaaagta aaattgttgt aggagaggaa aaagacgagt atatccatgt tgcgtctaat
     4141 aaggctcatt acttaaagaa ttttgtagaa aatctatttg aatgggtaaa attggattct
     4201 aaggaacaag tttttcattt tgatattttt gacttgaatg aattatcccg taatattata
     4261 tctgattgga tacctgtttt agaaagcagt cattttgaat atgaatttga tataccagaa
     4321 atagagtact tcttacgtat agacgcaaat gcatataccc gtatcattaa taacctatta
     4381 caaaatatta ttacccatag tagtggcaac aaaatgacat tgagaatttt tgaaaacaaa
     4441 gagcaagctc aaattataat aacagataat gggaaaggta tatcttcaga caatctccca
     4501 catatttttg aaaggttgta ccaatgcgac cattcacgag cttcaaaggg aaatggttta
     4561 ggcttggcga ttgcaaagga acttattaac gctcataagg gaaatattac cgccaacagc
     4621 actcccggta tgggaacaga atttacaata ttgcttccta aagccctgta aaagtacgag
     4681 gctttcttac tctaaaacaa gataaaagca agattatggc aaggttttgg caaggttcat
     4741 gttttataat tctaattgta gttgattttg acgttgtatc aactatcttt tgtagaaaca
     4801 aatttattaa aagtgaggaa attatatatg aatgattttg tgattgaaac aaagcaactt
     4861 acaaaaattt atgggaatca aacagtagtt gacaaagtca atctccatgt aaaaaaaggc
     4921 cgaatatatg gtctgttagg acgcaacgga gctgggaaaa ccacaattat gaaaatgatt
     4981 ttaggattga cttcaatcac gtatggcgag gtagatgtat ttggagagaa tattaaggga
     5041 cgtgaaaaac aagtatatcc ccgtatcggt gcaattatcg aaacgccagg attttatcct
     5101 aatctgacag gaacggagaa cttggagatt tttgcaaaac tgcgtggtac agccgcccct
     5161 aattctgtaa aaagagcatt ggaaattgta ggcttacctt ataaaaataa aaagctgttt
     5221 agtaaatatt cacttggcat gaaacagcga cttggtattg ccaacgctat cctgcatgac
     5281 ccagaactgt tagttttaga tgagccaacc aatggtcttg accctattgg tattgcggag
     5341 atgagaaagt ttattaggga cttgagcgtt gagcatggaa agacaattct tatttccagt
     5401 catattcttt cagaaatctc attgttggct gatgatattg gaattatcga ccacggtgtt
     5461 cttttggaag aaagtagtat gagtgaacta gaaaagaaaa acagtaaata tatcctgcta
     5521 caagtttcag atattccgaa gacatcactt attttagagc gtcaatttaa cacgaaggat
     5581 tattccgttc aagatgacta cacgttacac ctttataaca ctaatctgga tatgggtgaa
     5641 atcaataaag cacttgtaat gcagaatgta attgttatta gttctcaact ttgtaatgat
     5701 actttagagg actactttaa aaaaatcaca ggaggagagg gcattgctta aacttattca
     5761 aacagaattt cttaaactgc gtcgtaggaa acttatttgg ctgatgttgt tgtctgctct
     5821 cattatgccg tttttcgcac tactatactt caattatttt ggtaaaacag gtgttgagcc
     5881 aacaaagttt tataaatggt ctgcatttgg ttatacactt tggattattt tgcccgttgt
     5941 tttgggtata ttttgcacca tgcttataca cgaagaaaac cagtatgata tgctcaaaca
     6001 gctttggatt gtacctatta gtaaaatggg atactttttt agtaaatttt tcgttgtttt
     6061 gatttactct atttgtttta tgctgattac agccgttgct tctgtgttat ttagtgtact
     6121 ttctggatat gtcgtattta gctgggcaag cattttatat ttgttgaaaa aatgtttaga
     6181 aattggtgta atcactgctt ttgtaatgtt gccgattttg gcaatcgcta catctcaaaa
     6241 agggtacatt ctcccaattt gtatcacatt ggtatatgcc ttttccggtt ttgttataat
     6301 gacaataaat atgtatttac acccattatc cagtatggga gttattatca tgagaaataa
     6361 agacattcca ggtcttatat tttcacagga aattaatgtc ccattagcat ttctttgtat
     6421 ttgtatttgg agtattgtat cagtattgtt tgcaaacatt gcactaggaa gaagaaagtg
     6481 aggtgtagat aatgtggaaa ttactttggg ctgaatgtca aaaaattcgt cgttcaaaaa
     6541 ttgtttggat tacagttttt gcatccatta tgatagcagt catagttttt gcaggaggtc
     6601 aggatgtata taatgaccca gatttacatt atggcttaaa gagcgtttat gacggttcac
     6661 ggtatataga caatgcaggt tggtatatgg acgcagtaca accttggtca actttttttg
     6721 ttcttcctgc tgtcattgcc ctattaggaa gctatattat ttgtcgtgaa gaagaggagg
     6781 acactattaa atccctgcgt cttattccca taaatgaaac aaatttaacc atagcaaaaa
     6841 tgattattac ttttatattc agtatacttc tttatttgtt actctttact ataacatttt
     6901 tgactgaatc cgttttacat ttttccgatt tatcgacaaa attggtttta agttgcatga
     6961 aagagtattt tcttgatggc ataggtgtat tctttgcaat ttctcccatt attgccttag
     7021 tatcgcgcat gaaaaaagga tattggcttg cattagtatt cacagaagtt tattctattg
     7081 ctggactatt tgcgggtatg tcaaatgtct tacaaacctt ttttcctatt gcggcaatat
     7141 ttaatatttc tggatactat ataactactg gagaaaaaat tatggggagt atcataagtt
     7201 tattactttg tgcctgcctt tctgcattta tactgaaagg gctaaaacac aatgaaaaaa
     7261 attgaaatat gtatatcaaa atgaaaaaga taaaaaaact gattattcta attgtattct
     7321 tgtttctttt tggcatgtta cactgttata ctgaatggaa attcaacaat gacccaccta
     7381 aaggggtaat agaaagaata aatgagtgga ctgataagat tggactggat tagtaactta
     7441 tgttaattat agtgaatgta atattagcaa gttattgttt cgtattttaa tactataact
     7501 ttaaatattt ggttttaatc ttccaaacga atataaaaaa actgtcacag aggcaacata
     7561 tttttgaata tttataaagt ggtagttttg attttcaaag ctgccgcctt ttcttctata
     7621 taagcaaact tatcctcaac aaagtgtaaa acatggtaca ttttaagaaa aaagtaccac
     7681 agacacaagc aataaaagaa acgccgagaa cctacaaaaa cgtagtgctt atgcagattt
     7741 acgacgtctt ctattttctc aaccgtctta atgaccttgt acaaaagaaa tttttctaat
     7801 attttttata atcttatctt gtgatgtttt ttatgtataa atgacttaat aacaaatcca
     7861 ataaaaatta tggatattgg tataattaga acgaggaatt tataatcatt ataatgtgac
     7921 attattctta cccaactctc agaaaaaata tatcctatac tgattaatga agtattccat
     7981 atggttattc ccacgataga tgatattaca aaagttataa tattttgacc tgctatacct
     8041 gctataaaag atatgtatgt tctacataaa gggattaatc tacatacaca aacagaaata
     8101 gaagagtatt tattaaaata atcttgagat gcaaatattc ctttctttgc ctttggacat
     8161 atctctatta ttttatttat gatagattta cctccccaat ttcccactgt ataacaaaat
     8221 atcgaaccaa gtatacctgc aattatactt aaaattaaaa ttgtcagaaa tccaaaatga
     8281 tttcttgcag caattgctcc acataaaggt aaaacaactt cgctaggtaa tggaaaacaa
     8341 gcatactcaa tcataattaa tacaaataca gagattaatc catattgtaa aataaaacta
     8401 ttgattacag acacttactt atctatctcc tttcttattt aaaggtttta agggtacatg
     8461 ttctaaggat tctcacccta tatttaaaga atcaatctct ttaaactatt atatg
//
LOCUS       DAFKMX010000403         7274 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.184, whole genome shotgun sequence.
ACCESSION   DAFKMX010000403 DAFKMX010000000
VERSION     DAFKMX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.184"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..455
                     /locus_tag="LZB08_003849"
     CDS             <1..455
                     /locus_tag="LZB08_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7317254.1"
                     /translation="QIKEGEQTSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDY
                     EPKTPEADNSVDADTVNDATAFLETFFKLYPTATEKELAYYVAGNVLEPIGRDCLYSE
                     LVNPIFTKDGDNVKVKFAVKSLDNQTKATQVSQYELVLHKDGNWKIIG"
     gene            544..744
                     /locus_tag="LZB08_003850"
     CDS             544..744
                     /locus_tag="LZB08_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317255.1"
                     /translation="MIKNRIKEFRARYDMKQEDLAKAVGVRRETIGNLEKGRYNPSLV
                     LAWNIAKTFHVTIEEVFTVIDD"
     gene            761..985
                     /locus_tag="LZB08_003851"
     CDS             761..985
                     /locus_tag="LZB08_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317256.1"
                     /translation="MLGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKV"
     gene            1047..1745
                     /locus_tag="LZB08_003852"
     CDS             1047..1745
                     /locus_tag="LZB08_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317257.1"
                     /translation="MGSVLIIDDDKELCTLMKKCIEQEEMSALVANGGTEGLRLIDEN
                     KNLFSLVILDVMMPDIDGFQVLEKIRETSNIPVLMLTAKSGEDDKVFGLRLGADDYLT
                     KPFSIKELMARVNSLIRRYTTLNPTFAEEDCITLKGMKIDKVNRTVLVNNISVELTSK
                     EFDLLTFLASNKGRIFTKKQIYTQVWKDDYAFDDNNIMSFISKLRKKIEADPEHPFYI
                     LTIRGVGYRFNKEA"
     gene            1748..2665
                     /locus_tag="LZB08_003853"
     CDS             1748..2665
                     /locus_tag="LZB08_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7317258.1"
                     /translation="MNTYLLIAFVLALVIIACLVYKLYHINEQISFIKEVLVDIKSGN
                     LNRRILVQENDITKQICYDINQIAINNQSQLIHQKQSEQAYKRLMTSLSHDVKTPLAS
                     LVGYLEAVESQIVIGKEKDEYIHVASEKAQHLKHFVEHLFEWVKLDSGEQIFHFEILD
                     LNELTRNIMTDWILILENSYFEYEVEIPEEEYQVRIDINAYTRIINNLLQNIIVHSEG
                     NKMMLRVLKDQQRIKVIITDNGKGIASDKLPHIFERLYQCDNSRSTKGNGLGLAITKE
                     LVNAHKGTITANSILNHGTEFILSFPKAL"
     gene            2817..3740
                     /locus_tag="LZB08_003854"
     CDS             2817..3740
                     /locus_tag="LZB08_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317259.1"
                     /translation="MSDTVIKTKQLTKKYGKQTVVNKVNLNVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTITTSGNVEVFGKNIDGNEKRIYSRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTASPNAVKHALEVVGLPYKDKKTFAKYSLGMKQRLGIANAILHDPELLILDEP
                     TNGLDPIGIAEVRDFIKKLSVEHGKTILISSHILSEIAVLADDIGIIDKGVLLEESSM
                     SELEKRNRKYISLQVSDIPKTILILERQFHVKDYSVQDEQTVQIYDTTLNMGEVNKAL
                     VMEDITVISSGLCNDTLEDYFKKITGGEGIA"
     gene            3733..4467
                     /locus_tag="LZB08_003855"
     CDS             3733..4467
                     /locus_tag="LZB08_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317260.1"
                     /translation="MLKLVKLEFCKLRRKKLIWLMLLSAFVMPFFAFLYFGYLGQTNI
                     DPRLFYKWSAFGCTLFIILPFILGLFSTMLMHDENRYDMLKQLWIVPVSKMGYFFSKF
                     FVVLIYSVCFMLITAFASVLFSVIPNYVVFEWSSVLFLLERCLEIAVLTSFAILPILA
                     ISSSQKGYIFPLCITLIYVFAGFLITPINSCLHPLSCISVIIARNEAILGLSLPQNNV
                     PLALLSICVWGIASTLLAKIKLSTRK"
     gene            4477..5226
                     /locus_tag="LZB08_003856"
     CDS             4477..5226
                     /locus_tag="LZB08_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317261.1"
                     /translation="MRILLWAEFQKLRRSNIIMFTIFSTILIAVIVLISGVTTVSEEQ
                     LTTAMAGWYMTITQVWATMFVLPAVIALLGSYMICREEQDDTLKSLRLIPVNETKLTT
                     AKMIVALVFSMLVYLLLFMITFVTEAILHFSDLSMAMFSEFLKMYLLEGIGVFFAVSP
                     IIAIVPYLKKSYWLALLLAEIYSFAGLFMSMSNTLKTFYPITAIFGISGYYETTIQDY
                     FCSLGILIFCGCISAILLKNLNHRQRKKANE"
     gene            5219..5410
                     /locus_tag="LZB08_003857"
     CDS             5219..5410
                     /locus_tag="LZB08_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBM7317262.1"
                     /translation="MNKLCRIRKGKIFFGVCGGLAKCFNVDVKIVRLLFTITGFIFPK
                     SIIAYIILMIVIPKEPKTR"
     gene            complement(5531..5890)
                     /locus_tag="LZB08_003858"
     CDS             complement(5531..5890)
                     /locus_tag="LZB08_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317263.1"
                     /translation="MRKNKETQTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGSHPSFQLFHDLVTMFNISVDEYFYPSEKVEKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            6122..6301
                     /locus_tag="LZB08_003859"
     CDS             6122..6301
                     /locus_tag="LZB08_003859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317264.1"
                     /translation="MITYRRTLRRSQLDNYFCLVLQSSYPLHELKIHIDSIYITVCLC
                     SYIQMGCFYFIEIIF"
     gene            6401..6820
                     /locus_tag="LZB08_003860"
     CDS             6401..6820
                     /locus_tag="LZB08_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBM7317265.1"
                     /translation="MKTSSFKQAIEAQFDCLSKKVIKRAVKKGYRDMKRREKRECPFS
                     DIPDYEQERFGTFDKYESDYTVFNILGIEVWVEDDQLSEALKTLTEKKRNIILLSYFM
                     DMSDSEISEFIKIPRSNVQYHRTKTLETMKKIMEERK"
     gene            6817..7050
                     /locus_tag="LZB08_003861"
     CDS             6817..7050
                     /locus_tag="LZB08_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317266.1"
                     /translation="MKKHEHLPFEVILSATEGEPEAVETVLKFYDGYISKLCLRKLYD
                     EYGNVCMVVDADLKNRVQTALLDMLMNFEIVVM"
BASE COUNT         2449 a         1091 c         1444 g         2290 t
ORIGIN      
        1 agcagataaa agagggagaa cagacaagca atgtgaaagc aacctatacc gtgaaagtcc
       61 atgtggacgc tgacggggac atggtaatcg ttcagaaccc tacccttgcc ccagcaatcg
      121 aaaaatcgga ctatgaacca aagacaccag aagccgacaa cagcgtggac gctgacacag
      181 tgaatgacgc tacggcattt ctggaaactt tctttaagct gtacccgaca gccacagaaa
      241 aggaacttgc ctactatgta gcaggaaatg tgcttgaacc tatcggcagg gactgccttt
      301 attctgaact ggtaaaccct atcttcacaa aggacgggga caatgtgaaa gtcaagtttg
      361 ccgtgaaatc ccttgacaat cagacaaagg caacacaggt atcgcaatat gagcttgtgt
      421 tacataagga cgggaattgg aaaattatag gataatcaag agtgttcatt tattgactta
      481 ttttcttttg tgcggtatat ttttatcgta atgtcggaaa tataccgcat tttgaggtgg
      541 acaatgatta agaacagaat taaagaattt agagcaaggt atgatatgaa acaagaggac
      601 ttggcaaaag ctgtcggtgt tcgacgggaa acgattggta atcttgaaaa aggacgatac
      661 aatccctctc tcgtattggc ttggaatatt gcaaagacgt ttcatgtaac gattgaagaa
      721 gttttcacag taatagacga ttgaatttag gagtgatacc atgttaggac taaaaaaacg
      781 agatattaag aaagccttga aagcaggtgc aaaagctggt ggtgtttcat tggctgatgt
      841 acgagctgat attgaagcga cgattgatga agctatgaat agtaccgacc cagaagtgca
      901 ggcaaatttc aaaaagtatt ttggaaataa acgccctaca ccagaagaat atatttataa
      961 aattacaaaa aagacaaaag tttaatgttg aaaaacctta acgaaaatta agctatgata
     1021 aaatctatgc agagaggagt tataacatgg gaagtgtttt gattatagac gatgataaag
     1081 aattatgtac gttaatgaaa aaatgtatag agcaagaaga aatgtcagct ttggtggcaa
     1141 atggtggtac agagggttta cgtttgatag atgaaaataa aaatttattt tctcttgtta
     1201 ttttagatgt aatgatgcct gatatagacg ggtttcaagt tttagaaaaa attagggaaa
     1261 caagtaatat tcccgttcta atgctcacag caaaaagtgg tgaggacgat aaggtttttg
     1321 gactgcgtct aggagcagac gattatttga caaagccttt tagtattaag gaacttatgg
     1381 ctcgtgtcaa ttcacttatc cgacgttaca cgactttaaa tcctacattt gcagaagaag
     1441 attgtataac tttaaaaggc atgaaaattg ataaagtgaa ccgtactgtt ttagttaata
     1501 atatttctgt tgaacttaca agtaaggaat ttgatttact tacatttctt gcttctaaca
     1561 agggacgaat atttacaaaa aagcagattt atacacaagt atggaaagac gactatgctt
     1621 ttgatgataa caatataatg tctttcatca gcaaattaag gaaaaagatt gaagcagacc
     1681 cagaacaccc cttttacatt ttgacgatac gaggggttgg ctaccgcttt aacaaggagg
     1741 cttaattatg aatacttatc tgcttattgc ctttgtttta gcacttgtaa taatcgcttg
     1801 tcttgtttat aaactttatc atattaacga gcaaatttct tttatcaaag aagtgcttgt
     1861 tgacataaaa tctggtaacc ttaacagacg tattctagta caagaaaacg atataactaa
     1921 acagatttgt tatgatataa atcaaatagc aatcaataat caatcacagc tcattcatca
     1981 aaagcaatcg gaacaagcgt acaaacgact aatgacaagc ctttcacatg atgtgaaaac
     2041 tccacttgct tctttagtcg gttatttaga agcggttgaa agtcaaattg taatcggcaa
     2101 agaaaaagac gaatatattc atgttgcgtc tgaaaaggca caacatctta aacattttgt
     2161 agaacactta tttgaatggg taaaattaga ctctggggag cagatttttc attttgaaat
     2221 tttggatttg aacgaattga ctcgtaatat tatgactgat tggattttaa tattggaaaa
     2281 tagttatttt gaatatgagg ttgagatacc agaagaagaa tatcaagtgc gaattgatat
     2341 aaacgcatac actcgtatta tcaacaatct tttacaaaat ataattgttc atagtgaggg
     2401 aaacaaaatg atgttgcgag ttctgaaaga tcagcaacga attaaagtta ttataacaga
     2461 taatggaaaa ggaattgctt ctgataaatt gccacatata tttgaaaggt tgtatcaatg
     2521 cgataattct cgctctacca aaggaaatgg tctaggattg gcaattacaa aggaacttgt
     2581 caacgcacat aaagggacaa ttactgcaaa tagtattcta aatcatggaa cagaatttat
     2641 cctatcattt ccaaaggctc tgtaaaaatg cagagctttc tttttgtgaa agcaagataa
     2701 aaacaaggtt acggcaaggt tttggcaagg ttcatatact ataatttagg tgtaagtggt
     2761 aattctaata aattgccact tagatagaac agtaaataga aagtgaggaa atttgtatga
     2821 gtgatactgt gataaagaca aagcaattaa ccaaaaagta cggaaaacaa accgtagtaa
     2881 ataaggttaa tcttaatgta aagaaaggta ggatttatgg cttgctggga cgtaacggag
     2941 ctgggaaaac aaccataatg aaaatgatat taggattgac aataactact tctggaaatg
     3001 tagaagtttt tggaaaaaat atagacggaa atgagaaaag gatatattcc agaattggtg
     3061 caattataga aacaccgggg ttttatccca atttgactgg aacagaaaat ttagaaattt
     3121 tcgctaagtt acgaggaaca gcttcgccta atgctgttaa acacgcatta gaagttgtgg
     3181 gtctgcccta taaagacaaa aagacgtttg cgaaatactc tcttggtatg aaacaacgtt
     3241 taggtattgc aaatgcgata ttacatgacc ctgaattgtt aattttggac gagccaacaa
     3301 atgggcttga cccgattggg attgcagaag tacgagattt cataaagaaa ctcagtgtag
     3361 aacacggaaa aacaatttta atttcttctc atattctttc tgaaatagca gtgcttgcag
     3421 atgatatagg aattattgat aaaggtgttc ttttagaaga aagtagtatg agtgagttag
     3481 aaaagcgaaa taggaaatat atttcgttgc aagtttcgga cattccaaaa acaatactga
     3541 tattagaacg tcaatttcat gtgaaagatt attcagttca agatgaacag acggtgcaaa
     3601 tttatgacac gacattgaat atgggagaag ttaataaagc tcttgtcatg gaagatataa
     3661 cagttatcag ttctggactt tgtaacgata ccttagaaga ttatttcaaa aagattacag
     3721 ggggtgaggg tattgcttaa gctggttaaa cttgaatttt gtaagttgcg tcgtaaaaaa
     3781 cttatttggt tgatgttact atctgctttt gtcatgccat tctttgcatt tttatatttt
     3841 ggttatttgg gacagacaaa tattgatcct agactatttt ataagtggtc tgcatttgga
     3901 tgtacattat tcattatact gccatttata ttaggcttat ttagcacaat gcttatgcat
     3961 gatgaaaata ggtatgatat gctaaaacaa ctttggattg tgccagtaag taaaatggga
     4021 tattttttca gtaaattttt cgttgtattg atttattccg tttgttttat gcttattaca
     4081 gcatttgctt ctgtactgtt tagtgtcatt ccgaactatg tagtatttga atggagcagt
     4141 gttttgtttt tgttggaaag gtgtctggaa attgctgttt tgacatcatt tgcaatacta
     4201 cccattttag caatatcttc ttcacaaaaa ggatatattt tccctctttg cattacattg
     4261 atttatgtat ttgctggatt tttaatcaca ccgataaatt cttgtctaca cccactgtct
     4321 tgtatcagcg ttattattgc aagaaatgaa gcaattttag gattgtcact tccacaaaat
     4381 aatgtgccat tagcactttt aagcatttgt gtatggggaa ttgcttctac tttacttgct
     4441 aaaatcaaat taagcacgag aaagtaaggt ggaaaaatga gaatactttt atgggcagaa
     4501 tttcaaaaac tccgccgttc caatattatt atgtttacta tcttttcaac aatcttaatc
     4561 gctgtaattg ttttgataag cggggtgaca acagtatctg aagaacagtt aacaacagct
     4621 atggctggct ggtatatgac aattacacaa gtctgggcga ctatgttcgt tcttccagcc
     4681 gtaattgctc ttttggggag ttatatgatt tgtcgtgagg aacaagacga tacattaaaa
     4741 tctttacgtt tgattccagt aaatgaaaca aaattgacaa ctgcaaaaat gattgttgca
     4801 ttggtattta gtatgctggt gtatttgtta ctttttatga ttacatttgt aacagaagct
     4861 attttgcatt tctctgattt atctatggca atgtttagtg aatttttaaa aatgtattta
     4921 ttagagggga ttggcgtgtt ttttgccgtt tccccgatta ttgcaatcgt accatatcta
     4981 aagaaaagtt attggttggc tctactttta gcagaaatct attcatttgc tggattattt
     5041 atgagtatgt cgaataccct gaaaactttt tatcctatca cagcaatatt tggtatttcg
     5101 gggtattacg aaacaactat tcaggattat ttttgtagtt tgggaatatt gattttttgc
     5161 ggttgtatct cagctatctt attaaaaaat ttaaaccata gacaaaggaa aaaggctaat
     5221 gaataaacta tgtagaataa gaaaagggaa aatctttttt ggggtttgtg gtggtttagc
     5281 aaagtgtttt aatgtagacg ttaaaatagt aaggttacta tttactataa cgggttttat
     5341 ttttcccaaa agcattattg catatatcat tcttatgatt gttataccga aagaaccaaa
     5401 aacacgttaa attataggtt attgtaactt acggattgtc ggctcttact tgctacctgc
     5461 ggagcagatc agcaaacaaa aaactagtag aacgctacta aaaagaaaaa tagggaaaac
     5521 aagaagctga ttacttcttg ctttccctgc tattcaagat accgtcaatc gtaccttgaa
     5581 tgatttttaa ttcgttatca ctcaataagt caagtgatgt ttctatctga cggcgagctg
     5641 tacttttctc aactttctct gatggataga aatattcatc aaccgatata ttgaacatgg
     5701 taactaaatc atggaataat tgaaaacttg ggtgtgaacc gatattttca atatctgcga
     5761 tatgtcgttc tccatagaat accttatcgc ctaaatcgtt acgggatagc cctgcttttt
     5821 cacgggcttc tctgattgcc agtcctaatg gtctgaaatc aaaggtttgt gtttctttat
     5881 tttttctcat tttaaaccac ctagcatgag tttaaacttc atgccatgta tttagaatgt
     5941 gttaaaagtg catatagtat gcagtaataa ttcgaaaggt attatcgtgt gcggtaaata
     6001 aaaaaaaccg ttcttagata atatgctttt gcacgtctta aaaacgataa cgggacggtt
     6061 ttggatataa ccaaagctgt ccttttcttt tggtatagct ctggtttgag ggggctatgc
     6121 catgattaca tatagaagaa cgctacgtcg ttctcaactg gataattatt tctgtctagt
     6181 gcttcaatca tcatacccat tgcatgagtt aaaaattcat atagatagca tttatataac
     6241 agtctgtttg tgtagttaca ttcaaatggg ctgtttttat tttatagaaa taattttttg
     6301 aaaatcttgg tagtttgcca cctctttggg aagttagggt gcggaaaggg ggaaatgaac
     6361 aaaagttcat ttcaatccag cacagaaagg aggtgagcaa atgaaaacat cttctttcaa
     6421 acaagccatt gaagcacagt ttgattgtct ttcaaaaaag gttatcaaac gagctgtcaa
     6481 aaaaggctat cgtgatatga agcgacgtga aaaacgagaa tgtccatttt ctgatattcc
     6541 agattatgag caggaacgct ttggaacatt tgataaatac gaaagtgatt acaccgtatt
     6601 taatatttta ggcattgaag tatgggttga ggacgaccag ttaagcgaag ctcttaaaac
     6661 attgacagaa aagaaacgca acattatttt gttgtcctac tttatggata tgtctgatag
     6721 tgaaatcagc gaatttatca aaatcccacg ttctaacgtc cagtatcata gaacaaaaac
     6781 actcgaaaca atgaaaaaaa tcatggagga acgtaaatga agaaacatga gcatttgcct
     6841 tttgaggtaa tcttatcagc cacagagggc gaaccagaag cagttgaaac cgttttgaag
     6901 ttttatgacg gttacatttc caaattatgc ttgcgtaaat tgtatgacga atacggtaat
     6961 gtttgcatgg ttgtggacgc tgatttaaaa aacagagttc agacagcatt acttgatatg
     7021 ctgatgaatt ttgaaattgt agtgatgtag tacacataag tgaagtttgg tacttccccc
     7081 tttcctgcca cttcacttgt cattctatta aaatcaattt gactagaatt gattttgata
     7141 ggctgaatag ccgatagttc tttgaaaatt gaataaagca gacagatacg tttgtgaggt
     7201 agcaagccac ggggactgta cgccacgacc tttccaaaag aaagcgagcg acccacgcag
     7261 tgatccgaga gcga
//
LOCUS       DAFKMX010000404         5508 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.218, whole genome shotgun sequence.
ACCESSION   DAFKMX010000404 DAFKMX010000000
VERSION     DAFKMX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.218"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..1000
                     /locus_tag="LZB08_003862"
     CDS             <1..1000
                     /locus_tag="LZB08_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBM7317267.1"
                     /translation="KYDIIPIGITREGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQ
                     NRVKNGIIEFLIPDAVSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGL
                     FELAGIQVIGCGTLSSALCMDKDKSHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLT
                     YPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNN
                     DLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCS
                     GFARVDMFLTPSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            997..1803
                     /locus_tag="LZB08_003863"
     CDS             997..1803
                     /locus_tag="LZB08_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBM7317268.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            1821..3959
                     /gene="vanT"
                     /locus_tag="LZB08_003864"
     CDS             1821..3959
                     /gene="vanT"
                     /locus_tag="LZB08_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBM7317269.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            4273..4893
                     /locus_tag="LZB08_003865"
     CDS             4273..4893
                     /locus_tag="LZB08_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBM7317270.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(5059..>5508)
                     /locus_tag="LZB08_003866"
     CDS             complement(5059..>5508)
                     /locus_tag="LZB08_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBM7317271.1"
                     /translation="DIAYKIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFG
                     SRFRHRGFTHSLIALLLIYSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKE
                     GIELFYPITINISLLPIKTNSKTEKFISKLLNFIVIFLIGYQFYILL"
BASE COUNT         2018 a          619 c          915 g         1956 t
ORIGIN      
        1 aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc attatattgg
       61 agattataat aatatccaga ataacacttg gtttgaaagg gatactgata taattccagt
      121 tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc ctgatgctgt
      181 ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata ttaaatacac
      241 taaacttgat tttgtatttc ctgtattgca tggaaaaaat ggagaagatg gaacagtgca
      301 ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt cttcagcatt
      361 atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta aagtaccaaa
      421 atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa aagacttaac
      481 ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta gtaaaattta
      541 taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata atgaagtaat
      601 aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa acaatgactt
      661 acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt atacagaaaa
      721 atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg attgtgagaa
      781 aagaatacaa gagacagcaa aaattattta tagaactctt gggtgttctg gatttgctag
      841 agtagatatg tttttaactc catctggtga aattattttt aatgaagtca atacaattcc
      901 tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat tgtcttttga
      961 agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat tttattagat
     1021 aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa agtttatgat
     1081 gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga tatagattta
     1141 attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac aaacatttta
     1201 caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag tggatatcgt
     1261 actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg aaaagacttt
     1321 actaaaaagt ttgtagcact tccaaatcac agtgaacatc agactgggct tgctattgat
     1381 ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta taatggtatt
     1441 tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta tcagtgtaaa
     1501 aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt gggatatccg
     1561 cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga atatattaag
     1621 agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat agaaatcttt
     1681 tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca atgtgtatat
     1741 caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa tagaaaggac
     1801 taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt gattatttta
     1861 ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta tcatatagta
     1921 aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca ttctttttta
     1981 tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa ctgaaatctt
     2041 ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt cccttaaata
     2101 tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag gacattgtat
     2161 ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt gcaagtattg
     2221 catactttct tgtaaaaaaa ctaggtttaa agtactcatt agtaatcact attgttattt
     2281 atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata ccactattaa
     2341 agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt atattttttg
     2401 cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat atgtcaacta
     2461 aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag ggaatgtttt
     2521 tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg ccatgtatgt
     2581 atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta ttgcgaacat
     2641 catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt atgttttcta
     2701 aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat atagtggtaa
     2761 gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct ctaaaaaaag
     2821 acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt gatttaaata
     2881 atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt gaattgatgg
     2941 cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact tgtgcaaacc
     3001 aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat ttacgtcgat
     3061 atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg gcaaaggaac
     3121 ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc tctttaaaca
     3181 aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac agactgggtt
     3241 ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac ttagatatat
     3301 gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat gtagacttta
     3361 caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa aaaggtataa
     3421 aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc aatcttaagt
     3481 gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca aatgatacga
     3541 ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta attcttatta
     3601 gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca agcagagata
     3661 gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat ttatcctgtg
     3721 gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga atttgcatgg
     3781 accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat acggcaataa
     3841 taataggaag agataattta tctgaattat ctgcttctga agttgcaaat aactcatgta
     3901 gtatatcaaa tgaattattg agcagagtag gaaggaggtt aaatgttata aagaagtaac
     3961 tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac tagacatttt
     4021 tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta agctataaaa
     4081 aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat atataataaa
     4141 ataattatac cttaactgta aacttatata gattatttac tttttaatat tagattcatt
     4201 ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa gttaaaaaag
     4261 gggaaaagca atatgcagta tatacaagaa ctacttgatt tgactcaaaa ttattgggtg
     4321 ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc agccttacca
     4381 cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg atgtttgtta
     4441 tcatggattg gctcaggact tgggacaact tcattattct tattaataag cagatttaat
     4501 gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat aagttggatg
     4561 gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat gcccggttgt
     4621 ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt gccagcaatg
     4681 ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga tatagaaggt
     4741 tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt gtcatggaag
     4801 attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca tcataaaaaa
     4861 catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac gtatcttaaa
     4921 atgatttagt actaaattat tttaagatac gttttatttt ttgtattaaa gtacaaaatt
     4981 tttagttctt ttgtaaacta aaatatataa aacttattta tacttagttc aaaatattgt
     5041 tatttatttt ttctacaact ataataaaat ataaaattga tatccaatca aaaaaataac
     5101 tatgaaattt aatagcttag atatgaattt ttctgttttt gagttagttt ttataggtag
     5161 taaagaaatg tttatggtaa tgggataaaa caattcaata ccttctttag taataaggtc
     5221 aagacacaag tgagatagat atcctattat aaacccactg gacaaacatg agaaaactat
     5281 attattgtta gtgaagattg tcagagattt aaaaaaacta taaataagta ataaggcaat
     5341 tagactgtga gtaaaccctc tatgtctaaa cctacttcca aataatttgt atattaacat
     5401 aaatttttta cttatgtaag aacctctcat atctatatct ggaaataaag aacctaaata
     5461 tgcaaaataa atataaatta taattaatac tattttatat gcaatatc
//
LOCUS       DAFKMX010000405         4957 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.235, whole genome shotgun sequence.
ACCESSION   DAFKMX010000405 DAFKMX010000000
VERSION     DAFKMX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.235"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            375..1067
                     /locus_tag="LZB08_003867"
     CDS             375..1067
                     /locus_tag="LZB08_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317272.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            1064..1999
                     /locus_tag="LZB08_003868"
     CDS             1064..1999
                     /locus_tag="LZB08_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7317273.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            2147..3070
                     /locus_tag="LZB08_003869"
     CDS             2147..3070
                     /locus_tag="LZB08_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317274.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            3054..3824
                     /locus_tag="LZB08_003870"
     CDS             3054..3824
                     /locus_tag="LZB08_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317275.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            3834..4616
                     /locus_tag="LZB08_003871"
     CDS             3834..4616
                     /locus_tag="LZB08_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317276.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT         1601 a          733 c          954 g         1669 t
ORIGIN      
        1 ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt atttcacata
       61 tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag taaatgtatt
      121 atcatgtttg ttttattcaa gcacacttat aatacgcttg attttttcac aatgatgaaa
      181 tatactaatg ggtgttatag gtataagaat agcattacac ttgaagaagt aagtgataaa
      241 tgagatataa atcctcatat gataaactac tatttcattg aggaacatat tctaagtgat
      301 gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga atgagtgtag
      361 aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt
      421 tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa ctattgccat tctgggagag
      481 atggactggt tgaactttca aaacaagatt atcagttagt agttctagat gtgatgatgc
      541 caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct
      601 taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga gctggtgcag
      661 atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta ctgtctttaa
      721 tccgacgtta cacccgtttt aatccaaaaa atgaggagcc acagcaactt tctttcaaag
      781 gtttgttgat tgaccttgac cagcatacag tgactaccaa tggcgaacct atgttgcttc
      841 cacccaagga atttggtgtg cttctttact gtgcaaagca tcagggtaag attttgacaa
      901 aacagcagat ttatgaagcc gtgtggggtg agccatatgt atatgatgac agcaatatca
      961 tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt
     1021 tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa tggacgaaca
     1081 aacgataatt cttttcttac tcttgctgtc gattggtcta ctttttcttt ccatatttgc
     1141 tttttccaag cttgggcata tttacaagcg cctaaaagat atagaggaga tactggctga
     1201 tgtacaaatt ggagacgaaa atcgtaaaat actaattaag ccatgtgatg ttatggctcc
     1261 attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa
     1321 aaagtcggat aaagctagca aacaacttat gacaagttta tctcatgatg ttcgtacacc
     1381 attaacaact ctgattggtt atctggatgc tgtacacagt ggtatagtta tcgaacaaga
     1441 acgagaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg attacattga
     1501 tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa tagaatctgt
     1561 agaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat tccatgaaaa
     1621 gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaacc tcgacccaga
     1681 tggatattcc cgtgtggtca ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg
     1741 gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg ttgaggatga
     1801 tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt ataagtgtga
     1861 taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc aaatggtgga
     1921 gcgaatgggt ggtcaggttt ccgtagaaag tgagattgga aagtataccg tatttacagt
     1981 gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca cagggggatt
     2041 ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta atgtaagatt
     2101 gaggtggtat gataacttta ataaacaaag gaggctttta ttatttatga ataatttaat
     2161 tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa
     2221 tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg cgggtaagac
     2281 gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa ttaaaatatt
     2341 tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat gtttgattga
     2401 atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct ttgcaaggct
     2461 tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg gtttgccaaa
     2521 caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt tggcgattgc
     2581 tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca atggacttga
     2641 tcctattggt attgcagaag taagaacgtt tattcgtgat ctctgcacag aacgaggtaa
     2701 aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag atgacattgg
     2761 cattattgac catggtattt tgttggagga agaaagtctt gcccaattag aagaaaaaaa
     2821 cagtaaatac atacattttg ctgtatctga tgcagcacag gcagcaaggc ttctggaaac
     2881 ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc tatatgatac
     2941 agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg aggtatcgga
     3001 atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg
     3061 aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga
     3121 tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggaca
     3181 acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac
     3241 caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat aatgatgtat
     3301 taaaaaatct tgcaacagtg ccagtgcaaa aaggaaaact ggctctggca aaattggctg
     3361 tgttgctgtt tatatcattg ttctactctg ttgcaggatt aggagctact atcattggag
     3421 gtttcattgt tggaatagta gagggggttg ctgtaaaatt aggaatgagc attgcttttg
     3481 gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca
     3541 gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt agtatatcct
     3601 taaatattat caattttgaa ccaaataacc caatattaag tgtattacct gctcctgtta
     3661 taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac
     3721 agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag
     3781 cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg
     3841 atataattac tacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg
     3901 ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg
     3961 accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt
     4021 ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa
     4081 ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg ggaaaattaa
     4141 ttgcggttgg aggaattact gtattgttaa gtcttttcag cttcttatgc acatttattt
     4201 tggggttgtt gttttgcaat atcaatatat ctgttacact cgttgtaaaa tccttagttc
     4261 agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt gcttattttg
     4321 gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac ggtttttgtg
     4381 gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc
     4441 tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt gctatgactt
     4501 tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag
     4561 ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata
     4621 aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg
     4681 tctatatgct catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt
     4741 ttgtatttat taaatcacga ctatcataat acttgcaata ggtatagcga aaagagagag
     4801 tactgtcata tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata
     4861 atatatttga aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa
     4921 attggcaaat gagataatat ataaatatag aaatagt
//
LOCUS       DAFKMX010000406         4405 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.249, whole genome shotgun sequence.
ACCESSION   DAFKMX010000406 DAFKMX010000000
VERSION     DAFKMX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.249"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(168..521)
                     /locus_tag="LZB08_003872"
     CDS             complement(168..521)
                     /locus_tag="LZB08_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317277.1"
                     /translation="MAKRPVEKYDFKAFGEAIKAARTGRKESRKQVSDEMYISPRYLA
                     NIENKGQHPSLQIFFELMLRYNISVDQFLLDTPTQKDTKRRQLDALLDDMSDNGIRIV
                     TATAKEISEVEKEDK"
     gene            683..871
                     /locus_tag="LZB08_003873"
     CDS             683..871
                     /locus_tag="LZB08_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBM7317278.1"
                     /translation="MEYMSCPEAAKKWGISERRVQILCREKRIQGVSKLGYMWLIPKD
                     AEKPIDGRTKQGKELYHE"
     gene            864..1556
                     /locus_tag="LZB08_003874"
     CDS             864..1556
                     /locus_tag="LZB08_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBM7317279.1"
                     /translation="MNKVLIIDDDKELCALIKRSVLSEDIEADFCNMGKEGLQKLKER
                     EYQLVILDVMMPGMDGFETLEEIRKESSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIVSLIRRYTRFNQQDGISQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            1563..2480
                     /locus_tag="LZB08_003875"
     CDS             1563..2480
                     /locus_tag="LZB08_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBM7317280.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDALIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGTGTEFTLLFPLAN"
     gene            2617..3543
                     /locus_tag="LZB08_003876"
     CDS             2617..3543
                     /locus_tag="LZB08_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBM7317281.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGALLEEES
                     LAELEQKSSKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            3536..4345
                     /locus_tag="LZB08_003877"
     CDS             3536..4345
                     /locus_tag="LZB08_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBM7317282.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLAFLLPCLVGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSIAFCLISTLSVALFCKLFHVGMVYGMAYKIYMSLIFGVLIVAASL
                     PIVVLIIYFNKSFLLSILLAFFYSIFNWGLMGTIGTSISAAKIAFLNSFPVMCVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTFHTIFIMAITVVLSLWLIIRFYKKWTR"
BASE COUNT         1392 a          755 c          899 g         1359 t
ORIGIN      
        1 cgaaaatacg gaaaagggta ctcttttcct atgcttgcta cgaaagtttt ttcctcactg
       61 gttactacaa cgcaatgctc tatatgccaa aaaagagcaa aagaaaaacg ctgcaatctg
      121 taaagattac aacgcttcct aaatcatatt caatttgccg gacacattta tttgtcctct
      181 ttttcgactt ctgaaatttc ttttgcagtt gccgttacaa tccgtatgcc attatcgctc
      241 atatcatcaa ggagagcgtc gagctgccgc cgctttgtat ctttttgggt gggcgtatct
      301 aaaagaaact ggtctacgga tatattgtaa cgaagcataa gctcaaaaaa gatttgcaga
      361 ctggggtgct gtcctttgtt ttcgatattt gcaagatagc gaggagaaat atacatttca
      421 tcacttacct gtttgcggct ttctttgcgc cccgtccgcg cagcttttat agcttctcca
      481 aatgccttga agtcatattt ttctaccggt ctttttgcca tatttattca cccagttaca
      541 ttttactgtt cccctttctc tttggatatg tctatacagt tccactaaat agccataata
      601 attcatattt atatacttgc tattgttcgt gtgtccgact ataattatta tgataagatt
      661 ttcagaaagg acgggtgttg ttatggaata tatgtcttgc cccgaagcag caaagaagtg
      721 gggaatttct gaaaggcggg tacagatact ttgcagggag aaacgcatac agggtgtttc
      781 aaaacttggg tatatgtggt tgataccaaa agacgcggaa aaaccgattg acggtagaac
      841 caaacaagga aaggagctat accatgaata aagttttgat tatagatgat gataaggaac
      901 tttgtgcgct gattaaacgc agcgtgctat cagaagatat agaagctgat ttttgtaata
      961 tgggaaaaga gggattacaa aaattaaaag agagagaata tcaacttgtg atactggacg
     1021 ttatgatgcc tggtatggac ggatttgaaa cattggaaga aattagaaaa gaaagcagcc
     1081 tgccgatttt gatgtttaca tcaaaaaatg acagtgcttc taaagtacgc ggtttaaggg
     1141 caggagctga tgattatttg acaaaacctt ttgacatgga cgaactgata gcccgtatcg
     1201 tttcccttat acgccgttat acccgtttca atcaacagga cggaatatca cagcaacttg
     1261 aatttgacgg gttgaaaatt gatattgaaa atcgttctgt tactacggaa aatggaactt
     1321 ttgaattgcc tccaaaagaa tttgacttac tcttatactg cgcgaaaaat caagggaaaa
     1381 ttctgacaaa gcaacagatt tatgaagaag tgtggagaga ggaatatttc tatgatgaca
     1441 gcaacattat ggcgattatc agccgacttc gcaagaaatt agaggtaaat cccggaagtc
     1501 caaaatatat tcaaacgatt aaaggtatcg gttatcgttt taacaaggag gtttgatttt
     1561 ctatggaagc agtcattttt ctatccataa taattgcact tttctctatt cttgtttcct
     1621 gctttgctat tcgacgtgtt aaaaaacaga tagccgagat aacagacgca ttgattgatg
     1681 taaaaaacgg aaatggaaac aggcgtattt tatctgcaac aaatgaactg gtagcccctc
     1741 ttgcctatga aataaatgag attgttgtgt cttatgagag caggctttct actgtccggc
     1801 agacagaaga aacaaaccga cagcttatga caagtctttc ccatgatgtt cggacaccgc
     1861 ttacaacgct tattgggtat cttgacgctg cacataaagg aattgttaca ggaaaagaca
     1921 gggacgacta tatcgaaaca gcccgccgca aagctcacga tttgaaagaa tatattgatg
     1981 ttctttttga ctggttcaaa ttaaattcta atgagtttgc tatggatata aatattgttg
     2041 aagctgcgga gctgacacga aatatcttga ttgactggat acccatattt gaagataagc
     2101 agatagatta caatattgat attcccgaac agcctttccg ggtaaaactt gatacggacg
     2161 gctatatgcg gatattgaac aatcttattc agaatgtaat ttctcacagt catgcggata
     2221 aaatagaaat aatcctgtca aagcaggaaa aaaatatgca aatccgatta gcagataatg
     2281 gaatagggat tgaaaaagaa gatttgaagc atatttttga acggctctat aaatgcgata
     2341 agggacggtc tgaaaaaggc agcggtcttg ggctttctat cgcacaccaa cttgtagaaa
     2401 aaatgaacgg cacaataaca gcaaatagca tacagggaac aggaacggaa tttactttgc
     2461 tttttccctt agcaaattaa tgttctcccg tccttttgac gggagcctta attattttta
     2521 ggacaatatt tcaaattgca aggttaatgc aaggttgcgg caaggttaat gcaaggttgg
     2581 tatgatagaa tactttacag aataggaggt ttgaatatgg acaaaaatta tatcattgaa
     2641 acaaaaaatc ttaccaaaca gtacggctca caaaagagtg tcgctgactt aaatattcat
     2701 gtgcagaaag ggagaattta cggactgctt ggaagaaacg gagccggaaa aacgaccact
     2761 atgaaaatgc tacttggatt gacaaagcca acttccggcg aagttaaaat atggggaaag
     2821 tctttgcagg ggaatgaaaa gaaattgttg ccccgtatcg gtagtctgat tgagtcgccc
     2881 ggtttttacc ctaatttgac tgctacggaa aacttacaaa tttttgctac cttacggggc
     2941 atacccaacc gtcattcaat taaaaacaca ttggatttgg taggattgcc ttataaagac
     3001 aaaaaactgt tttcccagta ttctcttggt atgaaacaac ggcttgcgat tgcacttgca
     3061 gttatgcacg accctgaact tctgatttta gatgaaccta tcaatgggct tgacccgatt
     3121 ggaattgcag aagtacgttc ttttattcgc gagctttgcg atacaaaggg aaaaacaatt
     3181 ttaatttcca gtcatatcct ttcggagatt tctttgctag cagatgatat tggaattatc
     3241 gaccatggcg cactattgga agaagaaagt cttgcggaac tggaacaaaa aagcagcaaa
     3301 catatccgat ttactctttc tgataccgca caggcagcaa gaattttaga gcgtactttc
     3361 catgagagcc atttctctat acaggacgac cacaatttac gcttgcataa tcttgatttg
     3421 tccgtaggaa aaatcgtgac tgcttttgta gaaaatggat tggaggtatc agaagcccat
     3481 acttgtgaag aaagtcttga agattacttc aagcgtgtga cgggaggcga gggaattgct
     3541 taaactaatc aaatgcgaat tttggaaatt aaaacggaaa agattattat atgcactgat
     3601 gtcgttatcg ttattttttc cgttgatttt ggcatatatg gcaaaggcgg gaacaggtgc
     3661 agatacaacg gaacattatc ttcaaacccg atttgattat gtttatacaa tgatgttggg
     3721 atatggctta gcttttttgc tcccatgtct agttggtatt attgccgcta ttttgttttt
     3781 tatagaaaga gattgcgata cttccaaaaa ccttagaaca attcctgtta caaatacaca
     3841 gctcattatg gcgaaaattt ctatgctctt tattttcagc attgcatttt gtctgataag
     3901 cacattatct gtcgcattgt tctgtaaact gtttcatgta ggaatggttt atggtatggc
     3961 ttataaaata tatatgagcc ttatttttgg agttcttatc gtagcagctt ctttgccgat
     4021 tgtagtttta attatttact ttaacaaaag ttttttgctt tctatcctgc ttgcattttt
     4081 ttattctatt tttaactggg gcttaatggg aacgattggg acatctataa gtgctgcaaa
     4141 aattgctttc ttaaattctt tcccagttat gtgtgttatg aactggacaa gcggcttaat
     4201 gatggataat ttgcaaaaag ataatctttt gccggaagcc tatgcgattg taccaactac
     4261 tttccataca atttttatta tggcaattac agttgtactt tctttgtggc tgataattcg
     4321 cttttataaa aaatggacga ggtaaaaaat atgagaaata taataaaaac tgaattttta
     4381 aaattaaaac gatattctgt aataa
//
LOCUS       DAFKMX010000407         4241 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.251, whole genome shotgun sequence.
ACCESSION   DAFKMX010000407 DAFKMX010000000
VERSION     DAFKMX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.251"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..207
                     /locus_tag="LZB08_003878"
     CDS             <1..207
                     /locus_tag="LZB08_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBM7317283.1"
                     /translation="KLDLNDIIKPQQAMEIALKEQNGMVKEWSLDKDLDVTFYKIRID
                     KDKNEYDIKVDSKKGTVLKVEKED"
     gene            315..2846
                     /locus_tag="LZB08_003879"
     CDS             315..2846
                     /locus_tag="LZB08_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBM7317284.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKKALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNIKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3280..3981
                     /locus_tag="LZB08_003880"
     CDS             3280..3981
                     /locus_tag="LZB08_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBM7317285.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            3971..>4241
                     /locus_tag="LZB08_003881"
     CDS             3971..>4241
                     /locus_tag="LZB08_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HBM7317286.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFF"
BASE COUNT         1650 a          412 c          794 g         1385 t
ORIGIN      
        1 aaattagatt taaatgacat aattaaacct caacaagcta tggaaattgc attaaaagaa
       61 caaaatggaa tggttaaaga atggagttta gacaaagatt tagatgttac tttttataaa
      121 ataagaatag ataaagataa aaatgaatat gacataaaag tagattctaa aaagggaact
      181 gtattgaagg tagaaaaaga agattagaag atttaaacta tctttaaaat aatttctttt
      241 ttgaataaat tttgtttaaa atataacaag cagggtataa agagactaaa taactatttt
      301 aggaggagta cggtatgtca ttgaaatcac taaaaataaa agaaaattta tattgggtag
      361 gttcacttga ccctgattta agagtatttg acataattat gtatacacct tatgggacta
      421 cttataattc atatgtatta aaaggaacag aaaaaacagt tttatttgag acagttaaag
      481 ataagcattt tgacaattat attgaaagat taaatgattt aaatatagat tttgaaaaaa
      541 tagactatat tgttgtaagt catactgaac cagaccatgc aggaagcgtt gaaaagcttt
      601 tggatttagc taaaaatgca aaagtagtag cttcagaaac agcaattaaa tatcttaagg
      661 aaatcgtaaa caaagacttt gaatatgttg cagttacaga tggagataca ttatcaattg
      721 gggacaaaac tttagaattt ttctctgtac ctatgcttca ttggccagat accatataca
      781 catatataaa agaggataaa actcttgtaa catgtgattc atttggtagt cactatagta
      841 atgataagat agtaaataca cttgatgaaa atgaagaaaa ggattacttg gatgctttaa
      901 gatactatta tgattgtata atgggtccat ttaaaccatc tatggtaact gctatagaaa
      961 agataaaaga tttagatata gatactgtat gtccaggtca tggtccagta ttaactgaaa
     1021 atcctagaaa aattatagac ctttattaca actggagtgt aaatgaacaa ataaagttag
     1081 aaaaagaagt tacaatttgt tatgtttctg cacatggata tacaaagatt atggcagaag
     1141 ctataaaagc atatattgaa aaaaatagca actataaagt aaatttattt gatgtaatag
     1201 agcataagca agaagatatt ttggcaaaaa tagcagtatc acaaggtgta ttatttggaa
     1261 caccaacaat attaggagat gcgttaaagc ctatatggga tatattaata tcgttaaacc
     1321 cagttcttca tggtggaaaa gttgcttcag tatttggttc atatggatgg agtggagaag
     1381 gtatagaaaa tgctatggag agaataagtc agcttagaat gacagcagta aaaccttttg
     1441 ctgttaactt taaaccatca aatgaagaga ttgataaatt acattcttat acaggtaaat
     1501 tcctagataa gttaaattct acttttggaa gtaaaaagaa aactaagaaa tttaaatgtg
     1561 taatttgtaa tgaagttttt gaaggtgata gtgctcctag tgtatgccct gtatgtggag
     1621 ctaaggaaga ccaatttata gaagttgaag aagatgaagt tacttttaga aaagatactg
     1681 atgaatattt cgtaatagta ggaaatggag cagctggttt ttatgcagca gatgctataa
     1741 gaaaaagaaa taaaacttgt aagataacta tgatttctaa tgaagatgag ttgacttact
     1801 atagacctgc actttctgat ggtataaatg aagaacttgg ttcagatttc tacatggaag
     1861 ataaagattg gtatgataaa aataatatag tagtaatatt aggaacgaat gtagataaat
     1921 tagatgaagt gaataaaact attatagtga atgatggagc tattaaattt gataagcttg
     1981 taatagctac aggaagtaga aactttatac caccaattaa gggccatgat ttagaaaatg
     2041 tgtttactct tagaaatata aaagatttat atagtgtaaa aaaagcacta gaaaaatcta
     2101 agaaagttgt agtgattggt ggcggtttat tgggacttga agctgcatgg gaatttagac
     2161 taaaaggatt agaagtagta gtagttgaag ccatggatag catactatca aaacaacttg
     2221 ataaagaagg tagtaaaata ttagaacaat gtgtaagaga tacaggaata gatgtaagat
     2281 tgggtgtagc agttgatggt attgaaggtg atggaaaagc tcaaaaagtt gtatttaaag
     2341 atggagatag tgtcgactgt gatatggtag tattttcaat tggtgtaaga gcaaacactc
     2401 aaatggttca ggatacatct gtaaaaattg acagaggtat tgttgtagat aagacattac
     2461 agacaaatat taaagatata tatgcttgtg gagatgttgc acaagtggga aatattagtt
     2521 tagcaatatg gccatcttca gtagaaatgg gaaaaatagc aggggcaaat gcaagtggag
     2581 ataatttgac atttgaaagt gaagtatatc cagtttcatt agatgcaatg aatgtaaaag
     2641 tatttagtat tggtaatata caaaattttg ataaagaaat ttcatcaaaa gatgaaggtc
     2701 aaagaatata taaaaaatta tttatgaaag atggttcatt agttggagct atacttataa
     2761 atgatttaag ctgtactgtt aaattgataa gattaatatc tgaaaaaggt gattttgagg
     2821 atattatgaa ggctgatata ctataattta aatacattaa aataaaaagg agttgtctta
     2881 aaattgattt ttttctaatc aaattttaaa ttcaactcct tttttgaatt tttatataaa
     2941 ataacatgga aattttagta atgtctgtgg ttacatacat aggtttaatt gttttttata
     3001 tggattgtct ctggtataat aaattgaatt gtgtttgact gatagatatt tttattgaaa
     3061 gttaatacct gttatgcgat aataaggacg tgttagggga gtttttataa taacaattaa
     3121 tctcagtttt tgtgttccaa tggtagcatc aacacattct gatgtagtag ttcttttctt
     3181 ataaaaatta gttacaaata tgcttgtaat tatttgatat gtaaatttac caatattata
     3241 aatgatgacc tcgataataa ggagagtgaa aaataatcta tgaacgaaaa tattttgatt
     3301 gtggatgatg aaaaagaaat agcagattta attgaagtat atttaaaaaa tgaaggtttt
     3361 acagtataca agttttatac aggtaaggaa gctctagaat gtattgaatc taaaaatttg
     3421 gatatggcta ttttagatat tatgcttcct gatattgatg gatttcatat ttgccagaaa
     3481 attcgagaaa aatactatta tcctatcatt atgttaacag ctaaaattga agatatagac
     3541 aagataatgg gtttaaccat aggtgctgat gattatataa caaagccatt taaccctttg
     3601 gaagttgttg caagagtaaa gacacaatta agaagatata tgcgatataa taattcttat
     3661 gaacaacagt cgataattgt taatgagtat gatattaaag gattgattat caataaagaa
     3721 acacataaat gtagtttatt tgggaaagaa gtagcattaa caccgattga attttctatt
     3781 ctttggtatt tatgtgaaca tcaaggaaaa gtggttcctt ctgaagaatt gtttgaagct
     3841 gtatggggag aaaaatatct tgataataat aacacagtta tggctcatat agggcgatta
     3901 cgtgaaaaaa ttcatgaacc ttctaaaaac ccgaaattta taaaaactgt atggggagta
     3961 ggttatacaa ttgaaaagta aaaatattaa agtcaatcct gactttactc aagtgagaaa
     4021 taaaattttt atgagaacag ctttaatggc atttatagcc attattttca tatggatttt
     4081 atattctttt atattgtatg gtaagttttc tatctggata gtatcattct ttgaaaatgc
     4141 ttttcgtatg gaatataaag ctgcattgaa tttatatcag cagattttcc gtaatagcat
     4201 ggagctcttc atgatactag ctatggtgat tgtgtttttt a
//
LOCUS       DAFKMX010000408         2987 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.293, whole genome shotgun sequence.
ACCESSION   DAFKMX010000408 DAFKMX010000000
VERSION     DAFKMX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.293"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            complement(162..527)
                     /locus_tag="LZB08_003882"
     CDS             complement(162..527)
                     /locus_tag="LZB08_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBM7317287.1"
                     /translation="MIMKQNKEKFDFKAFGQAIKAARKAKGISRNQLADTLNIAPRYI
                     ASIENSGQHPSLQILYELVTLLDVSVDQFFFPEREQEKSTRRRQLDTMLDTMSETDLK
                     IMSATAKGIEEANNDRTGE"
     gene            832..1095
                     /locus_tag="LZB08_003883"
     CDS             832..1095
                     /locus_tag="LZB08_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBM7317288.1"
                     /translation="MKYKNAQAILPDALVKELQSYVQGEYIYIPVEQEQQKRWGEVSG
                     YRKELEQRNQKIKQEYQSGISINCLAEKYCLSLYAVRKIIYQK"
     gene            1229..1939
                     /locus_tag="LZB08_003884"
     CDS             1229..1939
                     /locus_tag="LZB08_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBM7317289.1"
                     /translation="MCTRFVYNGNDMITGFNFDIDLAVWKHKVIKEKERFYIGILRPD
                     GTYHSYHGINRNGNVGTLLYVHDNPAGIYRDNSDCITIADLTEQFIKAQISFDEVLQI
                     VKAKQVTYAPDATMQAMLSDSHGRVLIIEPGIGYKEEHEQYSLITNYSLMKPESTKDF
                     IVPGDDRYERALKKLEKYSPDFSISDAIHLLYDVRQEGAWATRVSFVYSTKEQTVYYV
                     ENNHFECIQTFTFPLERY"
     gene            1951..2571
                     /locus_tag="LZB08_003885"
     CDS             1951..2571
                     /locus_tag="LZB08_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBM7317290.1"
                     /translation="MLQKIYPRSKDKETVYLKSVITNPNIIVGDYTMYNDFVKEPKDF
                     EKNNVLYQYPINQDKLIIGKFCSIACGAKFIFNSANHTLSSLSTYPFPIFFEEWGLDV
                     KDITKAWDNKGDIVVGNDVWIGYEAVILAGVTIGDGAIIGARAVVTKDVPPYTIVGGV
                     PAKPIRKRFNEETIDILLKLKWWDWSKERIAKNIAAIQSGNVKQLN"
BASE COUNT          973 a          509 c          598 g          907 t
ORIGIN      
        1 cgaaaatacg gaaaagggta ctcttttcct atgcttgcta agaaagtttt ttcctcactt
       61 accactacaa cgaaattccc agtatgccaa aaaagcgcaa aagaaaacgc cgcaatcctg
      121 cgacgcttca atcactttat gcagacaaaa acgagggaac tttattcccc cgtcctgtca
      181 ttattggctt cctctatgcc ttttgcagta gcagacatga tttttaagtc tgtttcgctc
      241 attgtgtcaa gcatggtatc gagctgacgg cggcgtgtgg acttctcctg ttcccgctct
      301 gggaaaaaga actggtctac tgatacatca agcagggtta caagttcata taaaatctgt
      361 aaacttgggt gctgcccgct gttctcaatg gacgcgatat atcggggcgc aatattcagc
      421 gtgtcggcta actgattgcg tgatattccc tttgcttttc ttgctgcttt aatggcttgc
      481 ccgaaagcct taaaatcgaa tttctcttta ttctgcttca taatcatttc acccagttac
      541 attttactgt tcctattttc tttgagatat gattacacat atcatagtat ggatagaaat
      601 agagcatatt atattaaaag cagcgttgtc tttttgagat gaaaatgtta tactgacaat
      661 aatccctatt tgattgaaag aagtgattta tatgtgtatc aacaccaaaa agagaaacaa
      721 agattttctc catgcaaagc cagtagattg cgacggttgc atggagtaaa atagtcgcag
      781 aataaatgct ggctttgtaa cttgactatc ataatcaagg aggaagcctg catgaaatat
      841 aaaaatgcac aagctattct tccggacgca ttggtaaaag agttacaaag ttatgttcaa
      901 ggagaatata tttatattcc tgtcgaacag gaacaacaaa agcgttgggg agaagtttcc
      961 ggttacagga aagaattaga gcaacgtaat caaaagataa aacaggaata tcaaagcggt
     1021 atttctataa attgtcttgc cgaaaaatat tgtctatctt tatatgctgt tcggaaaatt
     1081 atatatcaaa agtaaggtaa ggggctgccg agtgtgcagc ctcttatttt tatacagatt
     1141 ggttcggtat tatatagagt atagaaaaat ttttcaacga actgtatgat aaagaagaaa
     1201 tatttaaaat acatgggagg gaactattat gtgtacccga tttgtttata atgggaatga
     1261 catgattaca ggatttaatt ttgatattga ccttgcagtg tggaaacata aagtgataaa
     1321 agaaaaagaa cgcttttata taggtatttt acgacccgac ggtacatacc actcttatca
     1381 tggtatcaat aggaatggaa atgttgggac attgttatat gtgcatgaca atcctgccgg
     1441 aatatatcga gataattctg actgtataac gattgccgac ttgacagaac agtttattaa
     1501 agcacaaatt tcttttgatg aggtactcca aattgtaaag gcaaagcaag ttacctatgc
     1561 accggacgca accatgcagg caatgttatc agacagtcat ggacgtgttt taatcattga
     1621 accgggaata ggctataaag aagaacacga acaatattcc ttgataacga attattcttt
     1681 gatgaagccg gaaagtacaa aagattttat tgttccgggg gacgatagat atgaacgtgc
     1741 tttgaaaaaa ttagaaaaat atagccccga tttttcaatt tcagacgcaa ttcatttact
     1801 ttatgatgta cgtcaagagg gcgcatgggc aacgagagtt tctttcgtgt attctaccaa
     1861 agagcaaacc gtatattatg tagaaaataa ccattttgaa tgtattcaaa cattcacttt
     1921 tccgttagag cgatactagg aggaaccatt atgttacaga aaatttatcc acgttcaaaa
     1981 gataaagaaa cggtatatct taaaagtgtt atcactaacc ctaatatcat agtgggcgat
     2041 tatactatgt ataatgattt tgtgaaagaa ccaaaagact ttgaaaaaaa taatgtttta
     2101 tatcaatacc ctatcaatca agacaaattg ataattggga aattttgttc aatcgcgtgt
     2161 ggagcaaagt ttattttcaa tagtgcaaat catacattat cgtccttatc aacataccca
     2221 tttcctattt tttttgaaga atggggatta gacgtaaaag acattacaaa ggcatgggat
     2281 aataaaggag atattgttgt cggaaatgat gtttggattg gttatgaagc agtcatttta
     2341 gcgggcgtta cgattggcga cggtgctatt atcggagcgc gagccgttgt aacgaaagat
     2401 gttccacctt atacaatcgt gggcggtgta cctgcaaaac ctatacgaaa aagatttaat
     2461 gaggaaacga ttgatatatt gctcaaatta aaatggtggg attggtctaa agagagaata
     2521 gctaaaaata ttgctgccat tcaatctggt aatgtgaaac aactaaacta aatctgccgc
     2581 gcgacggcaa aagaaaaaaa gccgtcgcaa tgcagacatc ttttaatgcg ttttttcagc
     2641 ggtggttttg aatttcagag ccgccgcttc tttttgcata aatttagcaa cgacgacctc
     2701 atttgacctt gcaatcagca tggcggtata taggaggttg ccgttatgct gaataaaata
     2761 agttatgctt atctgacaaa aggacgtctt ttgtcaaaaa aagtttgtat cctacaacgg
     2821 gttattccgg ctatgtagat gaactgttaa atcaattaaa tactgctgtt atttcctatg
     2881 cgtctgtctg cattttgcag gcaggcgcat ttcgcatttt acagaagatt tttcaaaaac
     2941 tttgccttct ttttggcaaa gcaaaaagtc gcctgtatta tcccgcg
//
LOCUS       DAFKMX010000409         1407 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.351, whole genome shotgun sequence.
ACCESSION   DAFKMX010000409 DAFKMX010000000
VERSION     DAFKMX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.351"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="LZB08_003886"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="LZB08_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBM7317291.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGYTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          601 a          116 c          228 g          462 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataaatctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggatatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatactctg taatatttat taaataattt tactaaattg aatctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatat
//
LOCUS       DAFKMX010000410         1313 bp    DNA     linear   BCT 30-DEC-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate Cd-510304-19
            SAMN24169333-rid13673343.guided.358, whole genome shotgun sequence.
ACCESSION   DAFKMX010000410 DAFKMX010000000
VERSION     DAFKMX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN24169333
            Sequence Read Archive: SRR17289433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001214236.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001214236.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/30/2021 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,810
            Genes (coding)                    :: 3,769
            CDSs (with protein)               :: 3,769
            Genes (RNA)                       :: 78
            rRNAs                             :: 7, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 8 of 41
            Pseudo Genes (incomplete)         :: 21 of 41
            Pseudo Genes (internal stop)      :: 15 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN24169333-rid13673343.guided.358"
                     /isolate="Cd-510304-19"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Austria: Vienna"
                     /collection_date="2019"
                     /collected_by="University of veterinary medicine Vienna"
     gene            <1..912
                     /locus_tag="LZB08_003887"
     CDS             <1..912
                     /locus_tag="LZB08_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBM7317292.1"
                     /translation="ELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGE
                     VALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLT
                     LLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRML
                     EQLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEI
                     TAVQEDDYIKLKFINYSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVE
                     LHNGTITAHSENNIITFKVIIPIVGNL"
     gene            1173..>1313
                     /locus_tag="LZB08_003888"
     CDS             1173..>1313
                     /locus_tag="LZB08_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HBM7317293.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REG"
BASE COUNT          509 a          147 c          231 g          426 t
ORIGIN      
        1 gagctcttca tgatactagc tatggtgatt gtgtttttta taatattccg tgtttattta
       61 aattggttta caaaatattt tagtgaaata aatcaaggta ttgactctct tatcaaagaa
      121 gatgttggag aagtggcttt atctccagag ttattggcaa tagaaaagaa aataaactca
      181 attaaacata tacttgaaca acgtaaattt gagacacaaa tggcagaaca acgtaagaat
      241 gaacttattg tttatcttgc acatgattta aaaacaccac ttacatcagt tatagggtat
      301 ctgacactac ttcgagatga aagtcagatt tctgaagaac tgcgaaaaaa atatttatct
      361 atttcgttgg ataaggcaga gcgtcttgaa gatttaatta atgaattctt tgaaattaca
      421 aggtttaatt tatcaaatat atcacttgaa tacagtacag taagcttaac tcgtatgctt
      481 gaacaactaa catatgagtt taaacctact ttgatgagta agaatttaaa atatacactg
      541 aatgttacac caaatatgat gataaggtgt gatgttaata aaatgcaacg tgtatttgat
      601 aacttattac gaaatgctgt ttattatagt tttgaagata ctactattga aataacagct
      661 gtacaggagg atgattatat aaagcttaaa tttataaatt atagcaatac gattccagag
      721 gaaaaacttg agcgtatttt tgaacaattt tacaggttgg atacatctcg taattcaaac
      781 agtggtagag ctgggttggg acttgctata gctaaagaaa tagtggagct tcataacggg
      841 acaataacag ctcatagcga gaacaatata attaccttta aggttattat tccgattgta
      901 ggaaatttgt aagaatttaa gtataaaaaa ataggaaatt ctttaggata aataaataaa
      961 gagcactctt gttattgata taattactgt atcaaaacaa gagtgctttt attttgatac
     1021 agtaattata tcaatagaaa aatgtgaaaa tatattatgt ggaacagatg caattgtaag
     1081 tgaaggttga ttggatttga aatagaaaaa gtattttaac atgtttctgt agattgtata
     1141 aaaataaata aatcaacaaa ggagaatcaa agatgaataa taaaaaaatt gcagttatat
     1201 ttggtggaaa ctcaacagaa tatgatgtat cattacaatc agcttttgca gtacttgaga
     1261 atttgaatac ggaaaaatat gatattatac caattggaat tacaagggag ggt
//
